LOCUS       DAJTOR010000001        89960 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTOR010000001 DAJTOR010000000
VERSION     DAJTOR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 89960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.001"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..731
                     /locus_tag="OO249_000001"
     CDS             <1..731
                     /locus_tag="OO249_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5634444.1"
                     /translation="QVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNIGANTT
                     FTLYVTKPFLGSMTIPKTDIAVIKGVWVDGMGSPSTGDFHDLVKLSIQGNLTAPQSCK
                     INQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMRIDGTT
                     GVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWP
                     VNLVGGELETGKFQGTATITVMVR"
     gene            complement(774..1634)
                     /gene="rsmI"
                     /locus_tag="OO249_000002"
     CDS             complement(774..1634)
                     /gene="rsmI"
                     /locus_tag="OO249_000002"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5634445.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1698..3725
                     /gene="lpoA"
                     /locus_tag="OO249_000003"
     CDS             1698..3725
                     /gene="lpoA"
                     /locus_tag="OO249_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5634446.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQPVP
                     VSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEEL
                     LKSNTPLNVLVLNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSS
                     LGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATTDSG
                     MTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAMLYAS
                     SRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDA
                     WSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3683..4078
                     /locus_tag="OO249_000004"
     CDS             3683..4078
                     /locus_tag="OO249_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5634447.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4098..4688
                     /gene="diaA"
                     /locus_tag="OO249_000005"
     CDS             4098..4688
                     /gene="diaA"
                     /locus_tag="OO249_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5634448.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4698..5273
                     /gene="yraP"
                     /locus_tag="OO249_000006"
     CDS             4698..5273
                     /gene="yraP"
                     /locus_tag="OO249_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5634449.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5393..5944)
                     /locus_tag="OO249_000007"
                     /pseudo
     CDS             complement(5393..5944)
                     /locus_tag="OO249_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            complement(6017..6652)
                     /locus_tag="OO249_000008"
     CDS             complement(6017..6652)
                     /locus_tag="OO249_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5634450.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6780..7298
                     /gene="yhbO"
                     /locus_tag="OO249_000009"
     CDS             6780..7298
                     /gene="yhbO"
                     /locus_tag="OO249_000009"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5634451.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7278..7721)
                     /locus_tag="OO249_000010"
     CDS             complement(7278..7721)
                     /locus_tag="OO249_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5634452.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     HRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7772..8074
                     /gene="yhbQ"
                     /locus_tag="OO249_000011"
     CDS             7772..8074
                     /gene="yhbQ"
                     /locus_tag="OO249_000011"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5634453.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8061..8564)
                     /locus_tag="OO249_000012"
     CDS             complement(8061..8564)
                     /locus_tag="OO249_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5634454.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8558..9082)
                     /locus_tag="OO249_000013"
     CDS             complement(8558..9082)
                     /locus_tag="OO249_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5634455.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADVSDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLHVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9291..10271
                     /locus_tag="OO249_000014"
     CDS             9291..10271
                     /locus_tag="OO249_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5634456.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10280..11158
                     /locus_tag="OO249_000015"
     CDS             10280..11158
                     /locus_tag="OO249_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5634457.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            11364..12371
                     /locus_tag="OO249_000016"
     CDS             11364..12371
                     /locus_tag="OO249_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR5634458.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12489..13733)
                     /gene="mtr"
                     /locus_tag="OO249_000017"
     CDS             complement(12489..13733)
                     /gene="mtr"
                     /locus_tag="OO249_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5634459.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13887..15776)
                     /gene="deaD"
                     /locus_tag="OO249_000018"
     CDS             complement(13887..15776)
                     /gene="deaD"
                     /locus_tag="OO249_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5634460.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15769..15849)
                     /gene="yrbN"
                     /locus_tag="OO249_000019"
     CDS             complement(15769..15849)
                     /gene="yrbN"
                     /locus_tag="OO249_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5634461.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15956..16840)
                     /gene="nlpI"
                     /locus_tag="OO249_000020"
     CDS             complement(15956..16840)
                     /gene="nlpI"
                     /locus_tag="OO249_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5634462.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDSDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16949..19084)
                     /gene="pnp"
                     /locus_tag="OO249_000021"
     CDS             complement(16949..19084)
                     /gene="pnp"
                     /locus_tag="OO249_000021"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5634463.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRSVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDICEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19332..19601)
                     /gene="rpsO"
                     /locus_tag="OO249_000022"
     CDS             complement(19332..19601)
                     /gene="rpsO"
                     /locus_tag="OO249_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5634464.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19750..20694)
                     /gene="truB"
                     /locus_tag="OO249_000023"
     CDS             complement(19750..20694)
                     /gene="truB"
                     /locus_tag="OO249_000023"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5634465.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITIYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20694..21095)
                     /gene="rbfA"
                     /locus_tag="OO249_000024"
     CDS             complement(20694..21095)
                     /gene="rbfA"
                     /locus_tag="OO249_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5634466.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21259..23979)
                     /gene="infB"
                     /locus_tag="OO249_000025"
     CDS             complement(21259..23979)
                     /gene="infB"
                     /locus_tag="OO249_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5634467.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREA
                     AEQAKREAAEKDKVSNQQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRV
                     AEEARRMAEENKWTDNAEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAA
                     RPKKGNKHAESKADREEARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITV
                     GELANKMAVKGSQVIKAMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEA
                     VMSDRDTGAAAEPRAPVVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVE
                     TENGMITFLDTPGHAAFTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVP
                     VVVAVNKIDKPEADPDRVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILL
                     QAEVLELKAVRKGMASGAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRA
                     MRNELGQEVLEAGPSIPVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKL
                     ARQQKSKLENMFANMTEGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSG
                     VGGITETDATLAAASNAILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAM
                     SGMLSPELKQQIIGLAEVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEG
                     ELESLRRFKDDVNEVRNGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24004..25491)
                     /gene="nusA"
                     /locus_tag="OO249_000026"
     CDS             complement(24004..25491)
                     /gene="nusA"
                     /locus_tag="OO249_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5634468.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(25519..25971)
                     /gene="rimP"
                     /locus_tag="OO249_000027"
     CDS             complement(25519..25971)
                     /gene="rimP"
                     /locus_tag="OO249_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5634469.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGHTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26178..26254)
                     /locus_tag="OO249_000028"
     tRNA            complement(26178..26254)
                     /locus_tag="OO249_000028"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26218..26220),aa:Met,seq:cat)
     gene            26602..27945
                     /gene="argG"
                     /locus_tag="OO249_000029"
     CDS             26602..27945
                     /gene="argG"
                     /locus_tag="OO249_000029"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5634470.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(27953..29578)
                     /locus_tag="OO249_000030"
     CDS             complement(27953..29578)
                     /locus_tag="OO249_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5634471.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFWAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMFRKEFTEDKQGNPPPEGQG"
     gene            complement(30037..30123)
                     /locus_tag="OO249_000031"
     tRNA            complement(30037..30123)
                     /locus_tag="OO249_000031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30087..30089),aa:Leu,seq:gag)
     gene            complement(30138..30470)
                     /gene="secG"
                     /locus_tag="OO249_000032"
     CDS             complement(30138..30470)
                     /gene="secG"
                     /locus_tag="OO249_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5634472.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30698..32035)
                     /gene="glmM"
                     /locus_tag="OO249_000033"
     CDS             complement(30698..32035)
                     /gene="glmM"
                     /locus_tag="OO249_000033"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5634473.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32028..32876)
                     /gene="folP"
                     /locus_tag="OO249_000034"
     CDS             complement(32028..32876)
                     /gene="folP"
                     /locus_tag="OO249_000034"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5634474.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32966..34900)
                     /gene="ftsH"
                     /locus_tag="OO249_000035"
     CDS             complement(32966..34900)
                     /gene="ftsH"
                     /locus_tag="OO249_000035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5634475.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35000..35629)
                     /gene="rlmE"
                     /locus_tag="OO249_000036"
     CDS             complement(35000..35629)
                     /gene="rlmE"
                     /locus_tag="OO249_000036"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5634476.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35755..36048
                     /gene="yhbY"
                     /locus_tag="OO249_000037"
     CDS             35755..36048
                     /gene="yhbY"
                     /locus_tag="OO249_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5634477.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36205..36681)
                     /gene="greA"
                     /locus_tag="OO249_000038"
     CDS             complement(36205..36681)
                     /gene="greA"
                     /locus_tag="OO249_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5634478.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36929..38362
                     /gene="dacB"
                     /locus_tag="OO249_000039"
     CDS             36929..38362
                     /gene="dacB"
                     /locus_tag="OO249_000039"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5634479.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMIATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVIPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38402..39574)
                     /gene="cgtA"
                     /locus_tag="OO249_000040"
     CDS             complement(38402..39574)
                     /gene="cgtA"
                     /locus_tag="OO249_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5634480.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39590..40555)
                     /locus_tag="OO249_000041"
     CDS             complement(39590..40555)
                     /locus_tag="OO249_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5634481.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40682..40939)
                     /gene="rpmA"
                     /locus_tag="OO249_000042"
     CDS             complement(40682..40939)
                     /gene="rpmA"
                     /locus_tag="OO249_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5634482.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40960..41271)
                     /gene="rplU"
                     /locus_tag="OO249_000043"
     CDS             complement(40960..41271)
                     /gene="rplU"
                     /locus_tag="OO249_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5634483.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41530..42501
                     /gene="ispB"
                     /locus_tag="OO249_000044"
     CDS             41530..42501
                     /gene="ispB"
                     /locus_tag="OO249_000044"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5634484.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42729..43007
                     /gene="sfsB"
                     /locus_tag="OO249_000045"
     CDS             42729..43007
                     /gene="sfsB"
                     /locus_tag="OO249_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5634485.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43055..44314)
                     /gene="murA"
                     /locus_tag="OO249_000046"
     CDS             complement(43055..44314)
                     /gene="murA"
                     /locus_tag="OO249_000046"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5634486.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(44369..44623)
                     /gene="ibaG"
                     /locus_tag="OO249_000047"
     CDS             complement(44369..44623)
                     /gene="ibaG"
                     /locus_tag="OO249_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5634487.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44783..45076)
                     /gene="mlaB"
                     /locus_tag="OO249_000048"
     CDS             complement(44783..45076)
                     /gene="mlaB"
                     /locus_tag="OO249_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5634488.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45076..45711)
                     /gene="mlaC"
                     /locus_tag="OO249_000049"
     CDS             complement(45076..45711)
                     /gene="mlaC"
                     /locus_tag="OO249_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5634489.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45730..46281)
                     /gene="mlaD"
                     /locus_tag="OO249_000050"
     CDS             complement(45730..46281)
                     /gene="mlaD"
                     /locus_tag="OO249_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5634490.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46286..47068)
                     /gene="mlaE"
                     /locus_tag="OO249_000051"
     CDS             complement(46286..47068)
                     /gene="mlaE"
                     /locus_tag="OO249_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5634491.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47076..47885)
                     /gene="mlaF"
                     /locus_tag="OO249_000052"
     CDS             complement(47076..47885)
                     /gene="mlaF"
                     /locus_tag="OO249_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5634492.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGCAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48095..49072
                     /locus_tag="OO249_000053"
     CDS             48095..49072
                     /locus_tag="OO249_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5634493.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49086..50072
                     /gene="kdsD"
                     /locus_tag="OO249_000054"
     CDS             49086..50072
                     /gene="kdsD"
                     /locus_tag="OO249_000054"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5634494.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50093..50659
                     /gene="kdsC"
                     /locus_tag="OO249_000055"
     CDS             50093..50659
                     /gene="kdsC"
                     /locus_tag="OO249_000055"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5634495.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSELLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50656..51231
                     /gene="lptC"
                     /locus_tag="OO249_000056"
     CDS             50656..51231
                     /gene="lptC"
                     /locus_tag="OO249_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5634496.1"
                     /translation="MSKARRWVTIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51200..51757
                     /gene="lptA"
                     /locus_tag="OO249_000057"
     CDS             51200..51757
                     /gene="lptA"
                     /locus_tag="OO249_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5634497.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51764..52489
                     /gene="lptB"
                     /locus_tag="OO249_000058"
     CDS             51764..52489
                     /gene="lptB"
                     /locus_tag="OO249_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5634498.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52537..53970
                     /gene="rpoN"
                     /locus_tag="OO249_000059"
     CDS             52537..53970
                     /gene="rpoN"
                     /locus_tag="OO249_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5634499.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTSETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53993..54280
                     /gene="hpf"
                     /locus_tag="OO249_000060"
     CDS             53993..54280
                     /gene="hpf"
                     /locus_tag="OO249_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5634500.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54398..54889
                     /gene="ptsN"
                     /locus_tag="OO249_000061"
     CDS             54398..54889
                     /gene="ptsN"
                     /locus_tag="OO249_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5634501.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54935..55789
                     /gene="rapZ"
                     /locus_tag="OO249_000062"
     CDS             54935..55789
                     /gene="rapZ"
                     /locus_tag="OO249_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5634502.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55786..56058
                     /gene="npr"
                     /locus_tag="OO249_000063"
     CDS             55786..56058
                     /gene="npr"
                     /locus_tag="OO249_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5634503.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56272..56904
                     /gene="yrbL"
                     /locus_tag="OO249_000064"
     CDS             56272..56904
                     /gene="yrbL"
                     /locus_tag="OO249_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5634504.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAYLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56901..57629)
                     /gene="mtgA"
                     /locus_tag="OO249_000065"
     CDS             complement(56901..57629)
                     /gene="mtgA"
                     /locus_tag="OO249_000065"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR5634505.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHRGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57626..58279)
                     /gene="elbB"
                     /locus_tag="OO249_000066"
     CDS             complement(57626..58279)
                     /gene="elbB"
                     /locus_tag="OO249_000066"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5634506.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58509..60845)
                     /gene="arcB"
                     /locus_tag="OO249_000067"
     CDS             complement(58509..60845)
                     /gene="arcB"
                     /locus_tag="OO249_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5634507.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60941..61870)
                     /locus_tag="OO249_000068"
     CDS             complement(60941..61870)
                     /locus_tag="OO249_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5634508.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62545..67005
                     /gene="gltB"
                     /locus_tag="OO249_000069"
     CDS             62545..67005
                     /gene="gltB"
                     /locus_tag="OO249_000069"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5634509.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67018..68436
                     /gene="gltD"
                     /locus_tag="OO249_000070"
     CDS             67018..68436
                     /gene="gltD"
                     /locus_tag="OO249_000070"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5634510.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEQFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENKDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68620..69099
                     /locus_tag="OO249_000071"
     CDS             68620..69099
                     /locus_tag="OO249_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5634511.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            69116..69421
                     /locus_tag="OO249_000072"
     CDS             69116..69421
                     /locus_tag="OO249_000072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5634512.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(69497..69961)
                     /locus_tag="OO249_000073"
     CDS             complement(69497..69961)
                     /locus_tag="OO249_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5634513.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPSEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(69958..70833)
                     /gene="nanK"
                     /locus_tag="OO249_000074"
     CDS             complement(69958..70833)
                     /gene="nanK"
                     /locus_tag="OO249_000074"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5634514.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(70830..71519)
                     /locus_tag="OO249_000075"
     CDS             complement(70830..71519)
                     /locus_tag="OO249_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5634515.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(71567..73057)
                     /gene="nanT"
                     /locus_tag="OO249_000076"
     CDS             complement(71567..73057)
                     /gene="nanT"
                     /locus_tag="OO249_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5634516.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVSGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(73166..74059)
                     /gene="nanA"
                     /locus_tag="OO249_000077"
     CDS             complement(73166..74059)
                     /gene="nanA"
                     /locus_tag="OO249_000077"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5634517.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(74181..74972)
                     /gene="nanR"
                     /locus_tag="OO249_000078"
     CDS             complement(74181..74972)
                     /gene="nanR"
                     /locus_tag="OO249_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5634518.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            75352..76718
                     /gene="dcuC"
                     /locus_tag="OO249_000079"
                     /pseudo
     CDS             75352..76718
                     /gene="dcuC"
                     /locus_tag="OO249_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(76761..77258)
                     /gene="sspB"
                     /locus_tag="OO249_000080"
     CDS             complement(76761..77258)
                     /gene="sspB"
                     /locus_tag="OO249_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5634519.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(77264..77902)
                     /gene="sspA"
                     /locus_tag="OO249_000081"
     CDS             complement(77264..77902)
                     /gene="sspA"
                     /locus_tag="OO249_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5634520.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(78297..78689)
                     /gene="rpsI"
                     /locus_tag="OO249_000082"
     CDS             complement(78297..78689)
                     /gene="rpsI"
                     /locus_tag="OO249_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5634521.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(78705..79133)
                     /gene="rplM"
                     /locus_tag="OO249_000083"
     CDS             complement(78705..79133)
                     /gene="rplM"
                     /locus_tag="OO249_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5634522.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(79351..80478)
                     /gene="zapE"
                     /locus_tag="OO249_000084"
     CDS             complement(79351..80478)
                     /gene="zapE"
                     /locus_tag="OO249_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5634523.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            80672..81070
                     /locus_tag="OO249_000085"
     CDS             80672..81070
                     /locus_tag="OO249_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5634524.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            81224..82591
                     /gene="degQ"
                     /locus_tag="OO249_000086"
     CDS             81224..82591
                     /gene="degQ"
                     /locus_tag="OO249_000086"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR5634525.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDCGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            82681..83748
                     /gene="degS"
                     /locus_tag="OO249_000087"
     CDS             82681..83748
                     /gene="degS"
                     /locus_tag="OO249_000087"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5634526.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(83811..84749)
                     /gene="mdh"
                     /locus_tag="OO249_000088"
     CDS             complement(83811..84749)
                     /gene="mdh"
                     /locus_tag="OO249_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5634527.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            85184..85654
                     /gene="argR"
                     /locus_tag="OO249_000089"
     CDS             85184..85654
                     /gene="argR"
                     /locus_tag="OO249_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5634528.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            86019..86282
                     /gene="yhcN"
                     /locus_tag="OO249_000090"
     CDS             86019..86282
                     /gene="yhcN"
                     /locus_tag="OO249_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5634529.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(86337..86609)
                     /locus_tag="OO249_000091"
     CDS             complement(86337..86609)
                     /locus_tag="OO249_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5634530.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(86701..88668)
                     /gene="aaeB"
                     /locus_tag="OO249_000092"
     CDS             complement(86701..88668)
                     /gene="aaeB"
                     /locus_tag="OO249_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5634531.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IIADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTERVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(88674..89606)
                     /gene="aaeA"
                     /locus_tag="OO249_000093"
                     /pseudo
     CDS             complement(88674..89606)
                     /gene="aaeA"
                     /locus_tag="OO249_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            complement(89614..89817)
                     /locus_tag="OO249_000094"
     CDS             complement(89614..89817)
                     /locus_tag="OO249_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5634532.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
BASE COUNT        22111 a        24155 c        22357 g        21337 t
ORIGIN      
        1 accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc gtatattctg
       61 ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc gttaatattg
      121 gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg atgaccattc
      181 cgaaaacgga tattgccgtc attaaaggcg tgtgggtcga tggaatggga agcccgtcta
      241 caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc gccccacagt
      301 cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc aatggtcaga
      361 agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt gatatcactt
      421 atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc gacggtacaa
      481 ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac aatgtgcccg
      541 atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct tttcagaacg
      601 gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc tggccagtta
      661 atctggtcgg tggtgagctg gaaacaggaa aatttcaggg cacagccacc attaccgtca
      721 tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca ctgttacccc
      781 tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat ttctgcgacc
      841 agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt gcgcagggca
      901 tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac catttcgcct
      961 ttgcgacggt tttcatcttc ctttacccac gccagcagtt cgccaacggg cgcgccgtga
     1021 atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga ttcgcctaat
     1081 accgcaacga tatcttccag gctatctaac agacggtggg tggattcata aaaaatcagc
     1141 gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga tttggcaggt
     1201 aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa cgcagtgata
     1261 gcagcacacg gcccgggtag cggcaccacg cgaatccccg cttcacggca ggtacgcacc
     1321 agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag cgcaatgttt
     1381 tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt atggtcgtgc
     1441 agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt gtgacgagta
     1501 tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg ggtgatatcc
     1561 gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt atccgccgat
     1621 tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt gcgtaaaaaa
     1681 atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc gcgcgttgtc
     1741 tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact cccgatcagt
     1801 ccactgctta tatgcagggc acagcgcagg ctgattctgc cttttatctt cagcagatgc
     1861 agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt gcactggtga
     1921 aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa gaactgaacg
     1981 attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg cagaaagatt
     2041 ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa caaaaccagc
     2101 aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc attgatttac
     2161 tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag cagaatattg
     2221 atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg ctggtgatca
     2281 acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg tttgataacc
     2341 gtaacgatcc cgatatgatg aaagccggga tcgccgactg gcagaaacgt tatccgaaca
     2401 atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt aaaccagcct
     2461 cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta tttggtcgca
     2521 ctattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg gcggctcagg
     2581 tagctgccgc agacgtagca gaacaacctc agccgcaaac tgcggatggc gttgccagcc
     2641 cggcacaagc ctcggttagc gatctgaccg gtgatcagcc tgcggcccag ccggtgcctg
     2701 taagcgcccc ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca tccgcagagc
     2761 tgaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag gttcagcagg
     2821 atggcgcgag tattgtggtt ggtccgttgc tgaaaaataa cgttgaagag ttgcttaaga
     2881 gcaacacccc gctgaacgtg ctggtactga accagccgga gaatatcgaa aaccgcgtca
     2941 atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg cgtcatattc
     3001 gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagttcattg ggcgatcgcg
     3061 tagccaatgc gtttgcgcag gagtggcaga aactgggcgg cggcaccgtt ctgcaacaaa
     3121 aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt atcgctttaa
     3181 cgggtacccc gattactccc agagcgacaa ccgactccgg catgacgacc aacaatccaa
     3241 cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt gtcgatgcgg
     3301 tgtacattgt ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc gccatgcgta
     3361 acggtagcca gagcggtgca atgctgtacg ccagctcccg cagcgcacaa ggcaccgcag
     3421 gcccggattt ccgtctggag atggaaggtt tgcagtacag cgaaatcccg atgctggcgg
     3481 gcggtaatct gccgttaatg cagcaggcac tcagcgcggt gaataacgat tattcactgg
     3541 ctcgcatgta tgcgatgggc gtcgatgcct ggtcgctggc aaatcatttc tcacaaatgc
     3601 gccaggttca gggttttgaa atcaacggta ataccggaag cctgacggca aacccggatt
     3661 gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta gtccccgcca
     3721 gttaaccacc aaacagaccg gcgatgcgtg ggaagtacaa gcgcgtcgct ggctggaagg
     3781 caaaggactg cgatttgtcg ccgctaacgt gaacgagcgt ggcggtgaga tcgatctgat
     3841 aatgcgtgaa ggctggacca ccgtttttgt cgaggtgcgt taccgccgct ctgcgcttta
     3901 tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac agactgcccg
     3961 cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt tcgatgtggt
     4021 agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc actcataatt
     4081 aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa agcattcaaa
     4141 ctcaaattgc ggcggcagag gcgcttccgg atgccatctc ccgtgcagcc atgacgctgg
     4201 ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact tccgctgcca
     4261 atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg cccagcttac
     4321 ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac gatcgcttac
     4381 atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat gtattgttag
     4441 ccatttccac ccgtggcaac agccgcgata ttgttaaagc agttgaagcc gccgttacgc
     4501 gtgatatgac cattgtggca ttgaccggct atgacggcgg cgaacttgca ggtttgttag
     4561 ggccacagga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt caggaaatgc
     4621 atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc cctcaccagg
     4681 atgattaagg agaatacatg aaggcattat cgccaatcgc agtccttatt tccgcgctgc
     4741 tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt accaaagccg
     4801 caactgaccc acgcagtgtc ggtacccagg tggacgatgg taccctggaa gtgcgcgtga
     4861 acagcgcatt gtcgaaagac gaacagatta agaaagaaac ccgtattaat gtcacggcgt
     4921 atcagggcaa agtgctgctg gttgggcagt caccaaatgc tgaactttcg gctcgcgcca
     4981 aacagattgc tatgggcgta gacggtgcca acgaagtgta taacgagatt cgtcagggcc
     5041 agccgattgg tctgggcgaa gcatctaacg atacgtggat caccaccaaa gtgcgttcgc
     5101 agctcttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc gaaaacggtg
     5161 aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca gatattgcca
     5221 gccgggtgag cggcgtaaag cgggtaacca cagcgtttac gtttattaaa tagcagcccc
     5281 ttgtaatgcc tgatgcgacg cttgccgcgt cttatcaggc ctacaacgat gcaaaccgta
     5341 ggtcggataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag aacaacagcg
     5401 ccagcccggc caccagacaa atcgccgcaa aaccccagcc gaggacaaac cccataaaca
     5461 ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca ccgcccatcg
     5521 atacctgttg gcgacgcatc cccgccgcga ccggagccgc acagcaggta cacatcatgc
     5581 ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac
     5641 gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca
     5701 ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc gcttgccacg
     5761 gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg gctgcagtaa
     5821 acgctttgcc gtagtaatgt tgccatttca cataccagag accggcaatg acaacgagaa
     5881 agaaaagcgc gggtttccac cactgaatgg gtgttgccgc ctgagatgaa gactgaccag
     5941 tcatagcatt ccctaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat
     6001 caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca
     6061 cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat
     6121 caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca
     6181 tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc
     6241 agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg
     6301 agtgggcatt ggcccccata gcactgacca ccagcatatg ctgcgcgccc agccgccgcc
     6361 cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttgctcc
     6421 ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt tcggtgacct
     6481 gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc atatcgccca
     6541 acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc aacatccgca
     6601 gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc
     6661 ctttacagaa ttgtctgcct tgcgctccac ggctcatgca ccagtcttaa tagaccatga
     6721 ggtaattatc cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca
     6781 tgagtaagaa aattgccgtt ttaatcagtg atgaatttga ggattcagaa tttacttcac
     6841 ccgcagacga gttccgtaaa gccggacacg aagtaattac cattgaaaag caagcgggta
     6901 aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc atcgatgaag
     6961 tgacgccagc ggagtttgat gccctgctgc taccgggcgg ccattcaccg gattatctgc
     7021 gtggggacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc aaacctgttt
     7081 tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac
     7141 tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgcggaa ttttacgatc
     7201 aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac gatctgccag
     7261 cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca aatcattttt
     7321 ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc
     7381 ggggccgaca gcattctggc aaccggaaag cgatgattgt acgccttgcg cgcgaggtcg
     7441 ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg aattaacgct
     7501 accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc actcatctgc
     7561 gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaacaga
     7621 taaaaagcat tagcgcacca cagttcccct tcctgctgca cacaccaggt gacaacatgt
     7681 tgttttgcca accaacggct gatggtgatg agtgtttcca ttgctgttct cttttatact
     7741 gtgggcctga ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg
     7801 taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca
     7861 gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt
     7921 ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg ttaagcaact
     7981 gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag aactgttgag
     8041 cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc aaccagaccg
     8101 gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct ttcagtgccc
     8161 ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg actgtacagc
     8221 gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct
     8281 tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac cgccaacggt
     8341 gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag
     8401 ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca
     8461 cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg cagcagggca
     8521 tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg
     8581 tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca
     8641 gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca
     8701 catgcagccc cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat
     8761 ctggatcttg tttacgcgcc gcaatcatca gcagatcgct gacgtcggca ctaaaactca
     8821 catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag gtaaaccatt
     8881 gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc
     8941 catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta
     9001 gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac
     9061 gggaacgaag tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc
     9121 catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc
     9181 accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc
     9241 cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt atggagctgc
     9301 tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg
     9361 tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg
     9421 agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg
     9481 cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgcgcc gtggatatgg
     9541 cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gaatacgccg
     9601 ccgagcgtta tccgaatatt gagcgccacg tatcggtgca ggcttcggcg accaatgaag
     9661 aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt
     9721 tgtcgattca tcaggtgaaa cagctggcac gggtcacacc tgtaccactg gaagtgtttg
     9781 ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg
     9841 gtgagtcgcc taacaccgtg ggcgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa
     9901 cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat caggacggcg
     9961 aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc
    10021 acgcgctgga agaaccaacc agtctcaata ccttggaact gctgccggag ttaatggcgg
    10081 cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg
    10141 tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa agcgcgtgga
    10201 tggagacgct cgggtcgatg tccgaaggca cgcagaccac ccttggcgcg tatcaccgta
    10261 aatggcagtg agaagagcaa tgaaatattc cttagggcca gtgctgtggt actggccaaa
    10321 agagacgctg gaagattttt atcagcaggc cgccaccagc agcgccgacg tgatttatct
    10381 tggagaagcg gtatgcagca agcgccgggc aaccaaagtt ggcgactggc tggagatggc
    10441 aaaatcgctc gccggaagtg gcaagcagat tgtgctctcc acgctggcgc tggtgcaggc
    10501 atcctctgaa ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc
    10561 cagcgatctc ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca
    10621 cgcgctgaac tgctacaacg cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg
    10681 ctggtgtatg ccggtggagc tttcccgcga ctggctggtg aatctgctta atcagtgcga
    10741 tgagctgggc attcgcaacc agtttgaagt ggaagtcctg agctacggtc atctaccgct
    10801 ggcctactcc gctcgctgct ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga
    10861 aacctgctgc attaagtatc cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt
    10921 gtttgtactc aatggcattc agaccatgag cggctacgtt tacaacctcg gtaacgagct
    10981 gacatccatg cagggattgg ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt
    11041 cgcgatgctc gacgccttcc gcgctaatga aaatggcgct gcgccactat tgttgacggc
    11101 aaacagcgac tgtaacggct actggcggcg gctggcagga ctggagctgc aagcctaagt
    11161 aaatatgctc gtcatacttc aagttgcatg tgctgcatct gcattcgttc accccagtca
    11221 cttacttatg taagctcctg gggattcact ctcttgtcgc cttcctgcaa ctcgaattat
    11281 ttagagcaca gctcactttg ttaacaactt taactactct ttaatgcagt attaaagatt
    11341 aatcggtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg ttactcgatc
    11401 tggcccccat tcccgaaggt tcttcagcgc gagaagcatt ctcccactct ctcgatctcg
    11461 cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga
    11521 ctggcattgc cagtgctgcc acgtcggtat tgattggcta tctggcggcg aataccacca
    11581 cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg
    11641 aacagttcgg cacgctcaat acactctatc cggggcgaat cgatttgggg ctgggtcgtg
    11701 cgccaggtag tgaccaacgg acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg
    11761 ataatttccc ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc gatcccaatc
    11821 cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca
    11881 gcctttacag cgcgcaactg gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact
    11941 tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagcaacttc aaaccgtcgg
    12001 cacgactgga aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc
    12061 gcgatgccga attcctgttt acctcaatgc agcaagcctt tgtgaagctg cgccgcggcg
    12121 aaaccgggca actgccgcca ccgattcaaa atatggatca gttctggtcg ccgtccgaac
    12181 agtatggtgt gcagcaggcg ctgagtatgt cgttggtagg tgataaagcg aaagtgcgtc
    12241 atggcttgca gtcgatcctg cgcgaaaccg acgccgatga gattatggtc aacgggcaga
    12301 ttttcgacca ccaggcgcgg ctgcattcgt ttgagctggc gatggatgtt aaggaagagt
    12361 tgttgggata gtgtgtctta acgcgggaag ccttatccga gctggcaacg ctgtcctacg
    12421 tagacctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc
    12481 accgctgatt actgatacac cggcagtaaa ttaaagctcg ataaaatatg taccaatgcg
    12541 ttgccgacgc caaacaccag aatcagcgta atcatcggct tgccgcccca gacgcggaat
    12601 ttcgggctgc caaagcgttt acgcgatgcg cgggccagca gtgccggaac aattgccgcc
    12661 cagatggtag ccgctaaacc agcataacca atggcgtaca ggaatccatt cgggaacagc
    12721 agaccaccca caacaggcgg tgcaaaggtc aacaatgctg ttttcaagcg gcccatagcc
    12781 gagtcatcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg
    12841 aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gaaggtccag gctacggctg
    12901 ttcagcacgc cgcttaatgc ctgtaccagc acatcaatat taccgccctt ctctgcaata
    12961 ccgataaact ccggacgcgg gatgttaccc atcgtcgcca gcaaccagat ggtatacagc
    13021 gccagtgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg
    13081 taatacttca tcaggcttgg cacgttaccg tgataaccaa acgacgccag gcagaacggc
    13141 aatgtcatca gcagatacgg cgcataagat gcattgcttt ctgcaacgtt gaacaatgtc
    13201 gcaggctgca catgccccaa caggctgcca aaggtgagga agaaggtaat gactttcgcc
    13261 cccagcacaa tcgccgtcat gcggctgacg gccttagtac tcaaccacac cacaaacgct
    13321 accagcagtg caaaaccaaa acccgccgcc cgtgccggga cgtttagcga catctctgcg
    13381 aaggtgtgat gcagaattga accactggca gaaatatagg cataggtcag gatatagagc
    13441 acaaaggcaa tggaaatacc gttgaccacg ttccagcctt tgcccagcaa atctttggtg
    13501 atggtgtcaa aactcgaacc gattcggtaa ttcaggttag cttccagaat catcaagccg
    13561 gaatgcagca tacagaacca ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc
    13621 ccggacatga ccactggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc
    13681 accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct
    13741 tctctccagt gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac cagtacgcga
    13801 gtacaaaaga cagaaaaaaa gccccgatgg taaaaatcgg ggctgtatat attattttac
    13861 agattgtgtt cgctgttcag cgatgattac gcatcaccac cgaaacgacg acgaccggta
    13921 gagtcatcac gacgcggagc gcggccttca cgacgttcgc cgctaaaacg acgaccatca
    13981 ccacggccac cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgttcgcca
    14041 ccgaaaccac gaccaccgcc acgacgctca ccgccagtat gcggctgtgc atcgcccagt
    14101 aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg cagcacttca
    14161 cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt gatgttacca
    14221 atgtaacggc tgctgatgtc gccttcgtta gcaatcgcac caacgatatg acgaacttca
    14281 acaccatcat cacggcccac ttcaatgcgg tacagctgca tatcgccaac atcacgacgt
    14341 tcacgacgcg gacgatcttc acggtcacca cgcgggccac ggtcgttacg atcgcgcgga
    14401 ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggtacg
    14461 atcagagtac gttcaccctg tgccattttc agcagtgccg cagccagagt ttcgagatcc
    14521 agctcttcac cttctgcagt cggctgaatt ttgctcagca gtgcgcggta ttgatccaga
    14581 tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg gcgtttgccc
    14641 agcagttctg cgttcggcag ttctacttcc ggaatagtca gcttcatagt acgttcaatg
    14701 ttgcgcagca gacgacgctc gcggttctca acgaacagca gcgcgcggcc agcacgaccc
    14761 gcacgaccgg tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag
    14821 ttaactacca ggctgatacg ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc
    14881 aggatgtcca gacgaccatc tttcaggcgt tccagtgtct gttcacgcag cgcctggttc
    14941 atgtcaccgt tcagcgcggc gctgttgtag ccgttacgct caagagcttc agccacttcc
    15001 agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg
    15061 aaacgtacca gtgcttcgtt tttgcgcata ccccagacag tccagtagct ctggctgatg
    15121 tcaggacggg tagtcacgct ggactgaatg cgcacttcct gcggctcttt cataaagcgg
    15181 cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct
    15241 tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg
    15301 tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg
    15361 tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt
    15421 aattgcacgt cataacgctg gccgccgtac agagcaacca catttacgcc gcgcatgtgt
    15481 ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc
    15541 accagaatct gtggtgcttt cagctcagga tcaagattct gcaacagagg taaagagaat
    15601 gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga
    15661 tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct
    15721 tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag
    15781 tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc
    15841 aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc
    15901 tcacccctta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt
    15961 gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg
    16021 cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg
    16081 tggcgctgtc cgaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac
    16141 tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt tccattaacg
    16201 tttgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat
    16261 ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg agcttctgct
    16321 cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca
    16381 gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc ccgcgattca
    16441 agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag
    16501 catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaacact tcaggcatat
    16561 ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac
    16621 tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc
    16681 gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca
    16741 atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa
    16801 gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac
    16861 ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc
    16921 ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg
    16981 gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct
    17041 ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg taatcggtca
    17101 ctttctcaac gcgtttgtca gcgatttgag agatgtggac cagaccttct ttaccgccgc
    17161 cgatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccagtg tagacgcggc
    17221 ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcatgtttc gctttctcgc
    17281 cgtcggtcgc tgcgatcttc acagtaccgt catcttcgat ttcgatggta gtgccagttt
    17341 cttcggtcag ggcacggata acagagccgc ctttaccgat aacgtctttg atcttgtccg
    17401 ggttgatctt gatggtatgg atacgcggtg cgaactcaca gatatcgcca cgcggcgcgt
    17461 tgatcgcctg ttccattacg cccaggatat gcagacgcgc acctttagcc tggttcagcg
    17521 caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc aatgcagaga
    17581 taccctcgcg ggaacccgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca
    17641 aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg
    17701 caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg
    17761 aagccataga agaggaaccg ttggattcag tgatttcaga cacaacacgt acggtgtacg
    17821 ggaatttgtc catatccggc atgactgcca gcacgctgcg cttcgccaga cgaccgtgac
    17881 caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacggag tacggaggga
    17941 agttgtagtg gaacaggaag gtatcggtac gttcgcccat cagttcatca agaacctgcg
    18001 cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg cgtttcacca cgggtgaaca
    18061 gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac atccagacca cggatcatat
    18121 ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacgtttt
    18181 tctcgatagc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa
    18241 gcagcgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt
    18301 cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg cgcgcgttca
    18361 gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg tttaccggct tctttcacca
    18421 gttcattgat gttctgaata acaacctgct gttgttcatg accgaacact actgcgccca
    18481 gcatctggtc ttcgctcagc agttcagctt cagattcaac catcagaaca gcggcttcag
    18541 taccggcaac aaccagatcc agtttgctct ctttcagctc gtcctgagtc gggttcagta
    18601 cgtactggtc attgatgtaa cctacgcgag cagcaccaat cggaccattg aacggaatac
    18661 cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacttgcg
    18721 ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca
    18781 gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttggacgac
    18841 cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt
    18901 taacggtcag tgggaagaag tcctgacctg gtttggcttt tttctggcca acaacggtaa
    18961 caaataccgc ggtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca
    19021 tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa
    19081 gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg ttaatacccg
    19141 atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc
    19201 tctcattagc cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa tacattagtt
    19261 tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggccccc ttttctgaaa
    19321 ctcggcagga attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg
    19381 tctttacgtt tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca
    19441 cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt
    19501 gcagtcagca gtgctacctg aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac
    19561 tcagaaacga ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata
    19621 tagaatgaaa ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt
    19681 aaacaattta cgcgacgtta agcggcagta ttctactcgt agcgacttgt tatcgcaaga
    19741 cggttaacat tacgccgggt attcaaccac caggcgacga ggcgcgacgc ggccttcatc
    19801 gtcaatttcg cccataccga taaacttgcc gttctcacct tctgtaacgc gaaccagtcc
    19861 ttccagcggc gcaccggatg tacgaactgg gttaccattt ttgaagtaaa cagaagacgt
    19921 taacggaaga ttcaccaccg ggtagtccga agctggactg tccattggca tcagtaatgg
    19981 atcaagtaac tccgcagccg gaatatcctg ctgttcggct tgttcgacca gttcacgcag
    20041 atgttccagg gtcaccatcc gttcaaccgg atatttactt accgccagac gacgcaggta
    20101 aataacatgc gcgccacaac cgagtttttc acccaggtca tcaatgatgg tgcggatata
    20161 agtgcctttt gagcagtgaa tttccagctc cagctcattg ccttcgtggc gaataaacag
    20221 caattcataa atggtaatcg ggcgcgcttc acgcggtact tcaatgccct gacgcgcata
    20281 ttcgtacagt tttttaccct gatatttcag tgctgaatac atcgaaggga tctgttcgat
    20341 atcgccacgg aaagtatcca gcgccgccgc cagttgctct gcgctaaagg ttaccggacg
    20401 ttcttcaacg atttgtccgt cggcatcaga agtatcggta cgctgtccaa gacgcgcaat
    20461 gacccgatag cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgaa
    20521 gcaaattggc aacatgccag ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt
    20581 ggcgttataa atccgtttca ctttttgcag cgcatcattg ctggacatac cctgcggttt
    20641 atccagcaac aaaacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc
    20701 tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc
    20761 aggtttgaca tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc
    20821 acgatacgca ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcttgc
    20881 aacgctttga tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat
    20941 actttggcat acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc
    21001 aggcgaggat ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc
    21061 gctacgcgct gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg
    21121 actgttagcc cctttttaaa attaatttca ggtggaaggg ctgttcacgt tggcctgata
    21181 agacgcgcca gcgtcacatc aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg
    21241 catggaaccc taaaaacctt aagcgatggt acgttggatc tcgatgattt cgaatacttc
    21301 gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg ccgataccac attccatacc
    21361 gttacggact tcgttaacgt catctttgaa gcggcgcagg gactccagct cgccttcgta
    21421 gataaccacg ttgtcacgca gaacgcggat cgggttgtga cgtttaacca caccttcggt
    21481 aaccatacag cctgcgatgg caccaaattt cggcgatttg aacacgtcac gaacttccgc
    21541 cagaccgata atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac
    21601 ttcgtcaatc aggttataga tgacggagta gtaacgcaga tctaggcttt ccgcttcaat
    21661 cactttacgt gcagaggcat cagcacgtac gttgaagcca accaggatgg cgttggacgc
    21721 cgcagccagg gtggcgtcgg tttcggtgat accacctacg ccagaaccga tgatcttcac
    21781 tttaacttcg tcagtagaca gtttcagcaa ggagtcggag atcgcttcca cagaaccctg
    21841 tacgtctgcc ttcaggacga tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa
    21901 catgttctcg agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg
    21961 acgatacagt gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc
    22021 cgcagccggt acgccggaca ggccgaggat ttccaccgga atggacggac ccgcttccag
    22081 cacttcctga cccagttcgt tacgcatcgc acgaacacga ccgtattcga agccacacag
    22141 aacgatatcg cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg
    22201 acctttatcg aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt
    22261 cagctccaga acttccgcct gcagcaggat agcgtccagc agttcatcga tgccggtacc
    22321 cgctttcgca gatacgtgta cgaactggct ttcaccgccc cactcttctg gcaggatgcc
    22381 atactgggag agttcgttct taacacgatc cggatcggct tctggtttat caatcttgtt
    22441 cactgcaacc accaccggta cctgcgccgc tttcgcgtgc tggattgctt cgatggtctg
    22501 cggcatcacg ccgtcgtcgg cagcaacaac caggactacg atgtccgttg cctgcgcacc
    22561 acgagcacgc attgaagtaa acgcggcgtg ccctggggtg tccaggaagg tgatcatgcc
    22621 gttttcagtt tcaacgtggt atgcaccaat gtgctgggta atgccgcccg cttcgccaga
    22681 ggccactttc gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc
    22741 catgatggtc acaaccggcg cgcgcggttc agccgcagcc cccgtgtcac ggtcgctcat
    22801 taccgcctct tccagctcgt tttcacgacg caggataact ttgtggccca tctcttcagc
    22861 aaccagctgt gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagttt
    22921 catcatcgct ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac
    22981 ggtgatggtt tcgccgatca caacgtcacg gttaacggcc tgagctggct tctggaagcc
    23041 ttgctgcagg gaagaacctt tacgttttcc gcctttacct ccacgtactg ctgcgcgagc
    23101 ttcttcacga tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgctgcttt
    23161 cgcgttacga ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg
    23221 gcgagcatgt tgagaagtag tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt
    23281 atcagtccat ttgttttctt ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc
    23341 ttcttcgagt ttacgacgcg cttcttcttc agctttacgc ttgagctctg cagcttcctg
    23401 ctcacggcgg gctttttcag cctgggcgtt tttagtcata tcgtcttgtt gattgctcac
    23461 tttgtctttt tccgcagctt cacgtttcgc ttgttcagca gcttcacgtt tcgcttgttc
    23521 agcagcttca cgcttagctt gttctgcggc ctcacgttca gctttttgtt gcgcctcgcg
    23581 tttagccgat tcttctgcct cacgacgggc ttgctcttcc gcttcacgct gcgcttgctc
    23641 ttccgcttca cgctgcgctt gctcttccgc tgcaaggcgt tcagcctctt gcggatcgcg
    23701 tttcacaaag gtgcgtttct tgcggacttc gatttgtacc gatttgcttt ttccaccggt
    23761 accaggaatg ttaagggtgc tgcgtgtttt acgttgcagc gtcaatttgt ccgggcctga
    23821 atttttctga ttcaggtggt caatcaaagt ctgtttctct tgtgcggaca cagagtcgtc
    23881 agcagacttc cggatacctg catcagcaaa ttgctgtacc aggcgttcca cggaggtctg
    23941 tcgctctgcg gccagcgttt taatcgttac atctgtcatg ctgttccttc ctgctacagt
    24001 ttattacgct tcgtcaccga accagcaaat attacgggca gccataatca gtgctccggc
    24061 tttttcgtcg gtcacccctt cgatatcagc cagatcatca atgccctgtt cggcgagatc
    24121 ttccagcgta caaacgccac gggcggccag tttgaatgcc aaatcacgat ctatcccttc
    24181 aaggttcagc agatcgtcag ccggtttgtt atcaccgagg ctttcttcct gggcctgtgc
    24241 aatggtggcc agtgcatttt tagcacgctc gcgcagtgct tcaacggtcg gctcatcaag
    24301 gccttcgatt tccaacagct ctttcatcgg cacataggcc agttcttcca gcgtcgagaa
    24361 gccttcttct accagaacag tcgcgaagtc ttcgtcgatg tcgagatatt tggtgaaggt
    24421 gtcgatcgct gcgtgcgctt ccgcctgatg cttagcctgc aggtcgtcaa cggtcatcac
    24481 gttgagttcc caaccgctca gctgcgaagc cagacgcacg ttctgaccgt tacggccaat
    24541 cgcctgcgcc aggttaccgg cttcaacggc gatatccatg gtgtgtttat cttcatccac
    24601 cacgatagaa gcaacgtctg ccggtgccat tgcgttaatc acaaactgcg ccgggttatc
    24661 atcccacagg acgatatcga tacgctcgcc gcccagttca gtagaaaccg cctgaacacg
    24721 cgcgccacgc atacctacac aggcacctac cggatcgata cgtttatcgt tggttttcac
    24781 cgcgattttc gcacgagaac ccggatcgcg agccgctgct ttaatttcaa tcacttcttc
    24841 gccgatttct ggcacttcaa tacggaacag ttcgatcagc atttccggct tggaacgagt
    24901 gacgaacagt tgcgcgccac gcgcttccgg gcgaacggaa tagagcacgc cacgaacgcg
    24961 gtcgccaggg cggaagtttt cacgcggcag catatcttcg cgcaggatca cggcttcagc
    25021 attgttgccc agctccagag agatgttgtc gcggtttact tttttcacca cgccggtgat
    25081 gatttcacct tcgtgttcac ggaactgatc aaccaccatc gcacgttcgg cttcacgcac
    25141 tttctgcacg ataacctgtt ttgccgtctg ggtagtgata cggtcaaagg taacagactc
    25201 aatctgatct tcaacgtaat cgcccaggtt caggctttca tcttcataac gtgcggcttc
    25261 aagggtgatt tccttggtcg gctgggtgac ttcatcaaca actaaccagc gacggaaggt
    25321 gtcaaaatca ccgcttttgc gatcgatctg tacgcggacg tcgatctctt gttcatattt
    25381 tttctttgtt gctgtcgcca gcgcgctttc caatgcttcg aaaatcttct cgcgaggtag
    25441 cgccttttca ttggatacgg cttcaactac agccaaaatt tctttgttca tcgcgggctt
    25501 ttcacctcat ccagactatt aaaagtgggg aaccaggttc gccttctgga tattactcag
    25561 cgcgaacact tcatctttac cttcgacggt aactgtgatc atttcaccgt ctaccgcttt
    25621 gataacgccc tgccatttac gacggttttg taccgccata cggagaacca gagtcacctc
    25681 ttctccgaca aaacgggcgt agtgttcagc cgtgaacagt gggcgatcga gacccggtga
    25741 ggagacttcc aggttataag caacggtgat gggatcttca acatccagca cagcacttac
    25801 ctggtggctc acatcagcac aatcatcaac attgatgcca tcttcactat caatatagat
    25861 gcgcagtgtg gatgtgtgac cgcgaataaa ttcgatgcca accagttcaa aacccagggc
    25921 ctcaactggc gcagtaatca tctctgttaa tttttgctct aatgtggaca agcccacccc
    25981 caagacataa aaaaagggcc taaagcccag ttattctgta gtcagataac aaaaaacccc
    26041 gataaatcgg ggctttatat aactgaaccc tataaccgca actgcggtct ggagcacttt
    26101 ccagaaggat tttttcaaat cccactacga aggccgaagt cttcacagta tatttgaaaa
    26161 aggactctaa gggaaagtgg ttgcgggggc cggatttgaa ccgacgacct tcgggttatg
    26221 agcccgacga gctaccaggc tgctccaccc cgcgcctgaa actcggcaaa ttctactcgt
    26281 tttgggtaaa agatgcaaat actgctggga tttggtgtac cgagacggga cgtaaaatct
    26341 gcaggcatta tagtgatcca tgccacattt tgtcaacgtt tattgctaat cacgtgaatg
    26401 aatatccagt tcactttcat ttgttgaata cttttgcctt ctcctgctct cccttaagcg
    26461 cattatttta caaaaaacac actaaactct tcctgtctcc gataaaagat gattaaatga
    26521 aaactcattt attttgcata aaaattcagt gagagcggaa atccaggctc atcatcagtt
    26581 aattaagcag ggtgttattt tatgacgacg attctcaagc atctcccggt aggtcaacgt
    26641 attggtatcg ctttttctgg cggtctggac accagtgccg cactgctgtg gatgcgacaa
    26701 aagggagcgg ttccttatgc atatactgca aacctaggcc agccagacga agaggattat
    26761 gatgcgatcc ctcgtcgtgc catggaatac ggcgcggaga acgcacgtct gatcgactgc
    26821 cgcaaacaac tggtggccga aggtattgcc gctattcagt gtggcgcatt tcataacacc
    26881 accggcggcc tgacctattt caacacgacg ccgctgggcc gcgccgtgac tggtaccatg
    26941 ctggttgctg cgatgaaaga agatggcgtg aatatctggg gtgacggtag cacctataaa
    27001 ggaaacgata tcgaacgttt ctatcgttat ggtctgctga ccaatgctga actgcagatt
    27061 tacaaaccgt ggcttgatac tgactttatt gatgaactgg gcggccgtca tgagatgtct
    27121 gaatttatga ttgcctgcgg tttcgactac aaaatgtctg tcgaaaaagc ctactccaca
    27181 gactccaaca tgcttggtgc aacgcatgaa gcgaaggatc tggaatacct caactccagc
    27241 gtcaaaatcg tcaacccgat tatgggcgtg aaattctggg atgagagcgt gaagatcccg
    27301 gcagaagaag tcacagtacg ctttgagcaa ggtcatccgg tggcgctgaa cggtaaaacc
    27361 tttagcaacg acgtagaaat gatgctggaa gctaaccgca tcggcggtcg tcacggcctg
    27421 ggcatgagcg accagattga gaaccgtatc atcgaagcga aaagccgtgg tatttacgaa
    27481 gctccgggga tggcactgct gcacattgcg tatgaacgtc tgttgaccgg tattcacaac
    27541 gaagacacca ttgagcagta tcacgcgcat ggtcgtcagt tgggccgtct actgtaccag
    27601 gggcgttggt ttgactccca ggcgctgatg ctgcgtgact ctctgcaacg ctgggttgcc
    27661 agccagatca ctggtgaagt taccctggag ctgcgccgtg ggaacgatta ttcaatcctg
    27721 aataccgtct cagagaacct gacctacaag ccagagcgtc tgacgatgga aaaaggcgac
    27781 tcggtgttct cgccagatga tcgtattggt caattgacca tgcgtaatct ggatatcact
    27841 gatacccgag agaaactttt tggttatgcc aaaactggcc tgctctcctc ctctgccact
    27901 tcaggcgtgc cgcaggtgga gaatatggaa aacaaaggcc agtaattcga catcaaccct
    27961 gcccttcagg gggggggtta ccttgcttat cctctgtaaa ctcctttctg aacatcacat
    28021 aatcgaaaac atcatggttt gcatccacaa cccgtgagtc tcctttatag tgtgcaatca
    28081 cttcctcaag cgtttgcggc tgttccggct ttgttacccc catccacgca ttaataaggt
    28141 aattattgta agctgtcgaa aagatgttgt tttccgttgt gcgatccacg ccatccccaa
    28201 gaacggggct ataccagata aacatcggga catgatatgc ctgctggcta gcctccctgc
    28261 ctccatgaaa atagacgttc ttcttcgtag ggtcacgttc caggccgtgg tcggcaaaat
    28321 acatgaccga ggcgcggcga tcttttaata attcaaaaac ctgacctagc aaactatctg
    28381 tgtaatgaat ggagttgtca tagcaggcat cctgatcgtc ctgcggttga aacacggcgc
    28441 tggattgcgg ataggcgcta caagccggtt catggcttcc atttaaatga agaacaatca
    28501 gtttcttttg ctgcgtattt tgctgtaatg cttgcgataa atgcggcaac aacaattcat
    28561 caaatcctct gacataaact gtttccatgg cgcgcatggc gatactggta actgcagtac
    28621 cattctgccg aaaagcggat tgcgagctta gccagaaagt ctgaaatcct gcttgattag
    28681 ccatattaat aatgttgtcg gggtaattat gaatgtcatg actcaaaaca gaatcagcag
    28741 ttaaagaaag gggaaccgac agcgcagtgt aaggtgcgcc gcttattgct tgattaaaca
    28801 gtttgatctg ttttctttgt gcttcaactt gcggtgtcgt agagcgtgta tatccataca
    28861 aagacatatt gtcgacacgt acagactccc ccacaatcaa cacgtaggta tcaatacctg
    28921 tatccctgac tgataattga aaatacggca ccgtgtttgc aattgagagt aatctttgat
    28981 gctctttcgc tgctaaagca aaatagttga gattgaaaaa cggcgtatag gtagcaaatc
    29041 gcgacgctag tatatatgga ctgaacgttt ttttattttt tgcatcttta taagcaaatt
    29101 gacaagcgga aaataaactg cccgaaatga caatcagcaa taatattcct gtcacttttt
    29161 ttgtcggcaa ggaaacatca tattttatta ttactgccca aaaaagaagg gataaaaaag
    29221 caaaggcaca tagataaggg atatacatcc ccagcatttt gacaacttca tccggatcac
    29281 tctggagcac actaatcgca aatccatcat tgaaagttgt accaaaggta caccatgaat
    29341 agaggctaat actcatgtct gccgcagtca gtaacacaaa tggtgcggca acaagcagac
    29401 gaagcggaag ggatgatata cgttttacaa ccaggatggt caggacgaag aacagcaatc
    29461 gctgaaccat atgcgcgccg gaagaggcga ctaagttcgt aataccaaaa acaataacac
    29521 aaagatacaa caaccaatga gatttaaaac ttctagcaaa tttgttgaat actgtcatcc
    29581 agaaatccaa gttcacaacc aatataaaca attatcaaca atgttcaatt ttatatcaga
    29641 catgtttttc aactctgatc catatcagac cgaaacactg ttagtttttt ctttttaaat
    29701 gatgcggcca ttgcgttctc agtaattaaa taacattatc aatgcgtctc taaacattac
    29761 gcagaacgta gccatcagta gttatactga caggagcagg atggtttatc gagaagtaaa
    29821 catatccgta ttcgtaccgg aactggagtt gacacaataa agtgccaatt atgtcagcag
    29881 aagggaaaag tttataacta aggcgtaccg gcaccatcgt ttcaaggtac cagctacgag
    29941 taaagcaact ggacgagata cagatacctg acaaccattc ctcagaccag gaccaaaacg
    30001 aaaaaagacg cttttcagcg tctcttttct ggagtttggt accgaggacg ggacttgaac
    30061 ccgtaagccc tattgggcac taccacctca aggtagcgtg tctaccaatt ccaccacctc
    30121 ggcacggata ctacttttta gttcgggata tcgctggtcg gcttagccgg agcagctggt
    30181 tgagtttgtt cggttttcgc cggtgcactc agattttccc attcgctacc tttattggtt
    30241 ttgttgctgt tgatgttacc cagcaccaga ctgatgatga agaataacgt tgccagcagc
    30301 gccgtcatgc gggtcatgaa gttaccagaa ccacttgaac caaacagcgt agcggaagcg
    30361 cctgctccga aggaggctcc catatcagcg cctttacctt gctgcagcat gatcagacca
    30421 acaaggccaa ttgccacaat aaggaaaact accaaaagag cttcgtacat aatcaacctg
    30481 ttccttgcgg agttgccgcg taccaatgct tcaaccaata aagcgggagt ttttaatctt
    30541 tcccactgaa gcgggtgtga atactaacca aagcgaatga ccttcgcaag ggcaatttta
    30601 tcgcattgta tcaactgcgg aaaaaaacag caaaaccgat gtattcgctg agaaaaaagg
    30661 cgacaatcgc cgccttttta gccagtcatt tatcgtttta aacggctttt actgcatcgg
    30721 cgatgcggtg tgcaaattca gtcacctgcg cttcgtcttc gccttccacc atcacgcgaa
    30781 ttaacggttc ggtgccggat ttacgcagca acacgcgtcc acggttgccc agcgcagctt
    30841 caacctctgc ggtcacggct ttaactgact catgctcaag cggatcgccg ctacctgcgg
    30901 tgtaacgcac gttaaccaga atctgcggga acattttcat gccgctgcaa aggtcgtgca
    30961 ggctcatatg gttacgtgcc atcgccgcca gcacctgcaa gccagcaacg atgccgtcac
    31021 cggtagtggt tttatccagc aggatcacat gaccggaatt ctctgcaccg atacgccagc
    31081 ctttctcctg cattttttcc agtacgtagc ggtcacccac tttcgcgcgc gcaaatggaa
    31141 tacctaactg tttcagcgcc agctcaagcc ccatgttact catcaatgta cccacagcac
    31201 caccacgcag ctggccctga cgaagacctt cacgcgcgat gatatacatg atctgatcac
    31261 catcgacttt attgccttca tggtcaacca taatcacgcg atcgccgtcg ccgtcgaagg
    31321 caataccgag atccgctttt tcagccagca cacgagcctg gagcgcgcga acgtcggtag
    31381 cccccacttc ggcattgatg tttacaccgt tcggctcaca accgatagcg ataacgttcg
    31441 cccccagttc acgcagcacg ttcggcgcga tgtgataagt cgcaccgttt gcgcaatcca
    31501 ccacaatctt cagttcgctg aggctaagtt cgttcgggaa cgtggctttg caaaactcga
    31561 tatagcgacc cgcagcatca acgatacggc tggctttacc cagttctgcc gaatcaacac
    31621 agctgatctc tttttccatt tccgcttcga tggcctcttc taccgcatcc ggtagtttgg
    31681 tgccgtcgat agagaaaaat ttaatgccat tatcgtagaa cgggttatgc gatgcagata
    31741 tcacaattcc ggcctctgcg cggaaggtac gcgtcagata agccacggcc ggtgttggca
    31801 tcgggccagt gaagagtgcg gaaaggcccg ctgccgccag acccgcttcc agtgctgact
    31861 ccagcatata gccagaaata cgcgtgtctt taccaataat gatcttacgg gagccgtggc
    31921 gcgccagcac tttacccgcg gcccaaccca gcttaagcac aaaatcaggt gtgatcggcg
    31981 catcccctac acgaccacga atcccatcgg taccgaaata tttacgatta ctcatagcgt
    32041 ttgttttcct ttgcagacag agtggcttcc accacccgca tcgcttctac ggtttctttg
    32101 acgtcatgaa cacgaataat gtgcgcgcct tgcattgcgg caatgaccgc acaggccaga
    32161 ctaccgctca ggcgctcgga cggccccacg ttcagcagct gcccaatcat cgattttcgt
    32221 gacataccta ccaacagcgg caggttgaaa tggtgaaatt cagccaggcg cgccagtaat
    32281 gaatagttat gggagagatt tttaccgaaa ccgaatccgg ggtcgagcaa caatttctct
    32341 tttgcgatac ccgcctgctc gcaacgtgct atttgctcaa taaagtagcg attcacttct
    32401 gcaaagacat cgtcatactt cggagcttcc tgcatggttt ttggatttcc ctgcatatgc
    32461 atcagacaaa ccggtaaacc ggtttctgcc gccgcctcca gagcgccagg ttcggaaagg
    32521 gagcggatat cgttaataat gtgagcgcca actttcgctg actcacggat gacttctggt
    32581 ttggatgtat ctaccgagag ccagacttcg aagcgttgag caattgcctc aaccacagga
    32641 ataacacgtt gcaactcttc ttcaacgcta acttccgccg cccctgggcg cgtggactcg
    32701 ccaccaacgt caatgatcgt cgcgccagcg ttgatcatca gattcgcatg tttcaccgca
    32761 tctatcagcg agttatgcgt gccaccatcc gaaaaggaat caggcgtgac gttgaggatc
    32821 cccattacgt gaggatggct aaggtccagt gaagtgccct gggcaaagag tttcatggtg
    32881 ttatccctgg tatgaattga taagaaaaaa ccccggagca cgccccgggg ttttcggtac
    32941 aaatacagtc atctgatgcg ggaacttact tgtcgcctaa ctgctctgac atggtgttac
    33001 ccgggttcgg cgtacgcggt tcatcaaccg gacgaggagc ctttggacta ccattgtcgc
    33061 cagaattgtt agaagcgcct ggttcttccc agcccgctgg cggacgtaca tcgcgacgtg
    33121 ccatcaggtc atcaatctgc ggtgcgtcga tagtctcata tttcatgaga gcatctttca
    33181 tcgcatgcag aatatccata ttgtcggtca gaagctgacg cgcacgatta tagttacgct
    33241 caatcagtgc tttcacttcc tggtcgatga tacgtgcagt ttcatcggac atatgtttcg
    33301 ctttcgctac gctacggccg aggaacactt caccttcttc ttccgcgtac agcagtggac
    33361 ccaatttctc agagaagccc cactgagtca ccatattacg cgccaggttg gtcgcaactt
    33421 taatatcgtt ggacgcaccg gtagaaacat gttccggccc gtagatgatc tcttctgcca
    33481 gacgaccacc gtacagcgta gaaatctggc tttccagttt ctgacggctg gcgctgattg
    33541 cgtcgccctc aggcaagaag aaagtcacac ccagcgcacg accgcgtggg ataatcgtca
    33601 ctttgtgcac cggatcgtgt tccggcacca agcgaccgat aatcgcatga cccgcttcgt
    33661 ggtaagccgt cgattctttc tgcgcttccg tcatcaccat ggagcgacgt tccgcaccca
    33721 tcatgatttt atctttcgct ttttcgaact caatcatcga cacaacgcgt ttgttgccac
    33781 gagcagcgaa cagtgccgct tcgtttacca ggttcgccag gtcagcaccg gagaaaccag
    33841 gagtaccacg ggcaatgatt gccgcgtcga tatcgggtgc caatggtacg cgacgcatgt
    33901 gaactttcag gatctgctca cgaccgcgaa catctggcaa gccaaccaca acctgacggt
    33961 cgaaacggcc aggacgcagc agggctgggt caagaacgtc cggacggtta gtcgcggcga
    34021 taacgatgat accttcgtta ccttcgaagc catccatctc aaccagcatc tggttcagag
    34081 tctgttcacg ttcatcgtga ccaccgccca gaccagcgcc acgctggcgg cctacggcgt
    34141 cgatttcatc gataaagatg atgcacggtg ccgctttctt cgcctgttcg aacatgtcac
    34201 gaacacggga tgcacccaca ccgacgaaca tttctacgaa gtcagaaccg gagatagtaa
    34261 agaacggaac tttcgcttcg cctgcaatcg ctttcgccag caacgtttta ccggtacccg
    34321 gaggaccgac catcaggacg cctttcggga tcttaccgcc gagtttctgg aagcggctcg
    34381 gctcgcgcag atactctacc agttcagcaa cttcttcttt tgcttcgtcg cagcccgcaa
    34441 cgtcagcaaa ggtcgttttg atctgatctt ccgtcagcat gcgcgctttg ctcttaccaa
    34501 acgacatggc acctttgcca ccgccgccct gcatttgacg catgaagaag atccagacac
    34561 caatcagcaa cagcatcggg aaccaagaga tgaagataga agccagcagg cttggttctt
    34621 caggcggttc accgacaacc ttgacgttct tggtcaacag gttatccagt aatttcggat
    34681 cctgaaccgg aatgtaagtg gtataacggt tactatcttt cttggtaacg ttgatttcac
    34741 gtccgttgat acgcgcttca cgaacctggt cgttattgac ctcttgtagg aaggtagagt
    34801 aatccacctt acggccatta gactcgctgg gcccaaagct ctggaatact gacatcagca
    34861 caacggcaat gaccagccag agtattaggt ttttcgccat gtcactcaag ggattaacct
    34921 cttattacaa ctgtgttaaa aacagcgtca ggatactcta tatccagcat ctttcaaact
    34981 ttcgtctgaa atctcccggt tagggtttac gcccggtcgc tacaatatac acttcccgcg
    35041 aacgtgcacg agaagagtcc ggcttacgaa ctttgacctt cgtaaacagg gagcgaattt
    35101 cccttagata ctcatcgaaa ccttcgccct ggaacacctt cactacaaaa ctgccacctg
    35161 gcgctaatac atcacgacac atttctaacg ccagttccac cagatacatg gcacggggga
    35221 tatccaccgc cggtgttccg ctcatgtttg gtgccatatc ggacatgaca acctggactt
    35281 tgctgtcgcc aacgcgctcc agcagtgctt tcatcaccag ttcatcacga aaatcgccct
    35341 gaagaaagtc cacaccaacg ataggatcca taggtaaaag atcgcaagcg atgatgcggc
    35401 ctttgccgcc aatttgggtg accacatatt gtgaccaacc acccggagca gcaccaaggt
    35461 cgacaaccgt cattcccggt ttaaagagtt tgtcactttg ctgtatttca tcaagtttaa
    35521 accaggcacg ggaacgtaac ccctttttct gtgcctgttg aacatattta tcgctaaagt
    35581 gttcctgaag ccagcggctg gagctggcag aacgcttctt acctgtcatt taactttccc
    35641 atggggataa ctcatcgtaa ccaattgcgt aaatttttac gcgcctattt ggtgatatat
    35701 gggagatggc ggtagaatga cccgttttca atcccaacgt aagcaaaaaa tacgatgaat
    35761 ctgagtacta aacaaaaaca gcacctgaaa ggtctggcac atccgctcaa gccagttgtt
    35821 ctgcttggca gtaatggttt gaccgaaggg gtgctggccg agattgaaca agcgttagag
    35881 caccatgaac tcatcaaggt gaaaatcgcc accgaagatc gcgaaacaaa aaccttgatc
    35941 gtggaagcta tcgtgcgcga aaccggcgcc tgtaatgtac aggtcatcgg taaaacgctg
    36001 gtgctttatc gcccaactaa agaacgtaaa atctcgctgc cacgctaaga ttatcctaaa
    36061 gttacacaca tttgctgtgt aaaacgaggg gttttccgca ggcaggagag caaaatgcca
    36121 cgctctgttc gttgataaaa ggccgcatag cggccttttt cctttcttta caatacatca
    36181 acatcttgat gtattgggta attcttacag gtattccacc ttaattactt caaattctac
    36241 ttcgccgccc ggcgttttga tgaccacaac atcatcttct tctttgccga tcaggccacg
    36301 agcaataggc gagtttacag aaatcaggtt ttgtttaaag tcagcttcgt catcgccaac
    36361 gatgcgataa gtctgttctt cgtcagaatc cagattcagc accgttacgg tagcaccaaa
    36421 aataacgcgc ccattgttgg gcattttggt gacatcaatc acctgcgcgt tcgacagctt
    36481 ggcttcgatg tctttaatac ggccttcgca gaaaccctgc tgttcacgag ctgcgtggta
    36541 ttcggcgttt tctttcaggt cgccatgctc acgcgcttcc gcgatagcag caatgatttc
    36601 aggacggcgc acagatttca gaaaatccag ctcttcgcgt aatttttcag cgccgcgtaa
    36661 ggtcatcgga atagcttgca tttgttatac ctcttgaata ttcctgatgg ggcaagtctt
    36721 cacccacccc ggctgttcgg cctgcccggc ataactcctc accgggacca gaagcaaaaa
    36781 aataccgacc cgggtacaag tcccaggtca gctacaattc acattttgat agtcatttta
    36841 ccctgaagtt cccgaagggt catcgtttac tttatagggc gttgcgccgt agtatgacgg
    36901 ctcgattcca ggttgttagc gcgagattat gcgattttcc agatttatca tcggattgac
    36961 cagctgtata gcgttcagtg ttcaggccgc gaatgttgat gagtacgtta ctcaactccc
    37021 cgctggtgcc aaccttgccc tgatggtgca aaaagtcggc gcgtcggccc ccgctattga
    37081 ttaccacagt cagcagatgg cgctgcccgc cagtacgcag aaagtgatta ctgcgctggc
    37141 ggcgttgatt caactcggcc ccgattttcg tttcaccacg acgcttgaaa ccaaaggcaa
    37201 tgtggaaaac ggcgtactta agggtgactt agtggcgcga tttggtgccg atccgacgtt
    37261 aaaacgtcag gatattcgca atatgatcgc gactttgaaa aaatctggcg tcaaccaaat
    37321 cgatggcaat gtgttgatag atacctccat tttcgccagc cacgataaag cccccggctg
    37381 gccatggaat gacatgacac aatgctttag cgctccgcct gccgctgcca tagttgaccg
    37441 caactgtttc tccgtctcgc tctacagtgc cccaaaacct ggtgatatgg cttttatacg
    37501 cgtggcatct tattaccccg ttacgatgtt cagccaggta cgcaccctcc cccgaggttc
    37561 tgccgaagcg caatactgcg aactggatgt cataccaggc gacctgaacc gctttacgct
    37621 gacgggatgc ctgccacaac gttctgagcc gctcccgttg gcttttgccg tgcaggatgg
    37681 agccagctat gccggggcaa ttctgaaaga tgagttaaaa caggcaggta tcacctggag
    37741 cggaacactg ctgcgccaga ctcaggttaa cgaacctgga acggtagttg ccagtaaaca
    37801 gtcggccccg ttgcacgatc tgcttaagat tatgctgaaa aagtcggaca acatgatcgc
    37861 cgatacggtt ttccgcatga taggccatgc gcgcttcaat gtgcctggaa catggcgggc
    37921 cgggtcggac gccgtgcgtc agatcctgcg ccagcaagcc ggtgtcgata ttggaaacac
    37981 cattattgcc gatggttcag ggctttcgcg gcataacctg attgcccccg ccaccatgat
    38041 gcaggtgttg caatacattg cccaacacga caatgaactt aactttatct ccatgctgcc
    38101 gctggcgggc tatgatggct ctttgcagta ccgtgcaggt ctgcatcagg cgggcgtgga
    38161 tggaaaagtc tcagcgaaaa ccggttcgtt gcagggggta tataacctgg cgggattcat
    38221 taccacagcg agcgggcaac gaatggcgtt tgtacaatat ctttctggct atgcagtaga
    38281 accggcggat cagcgtaatc gccgtattcc gttagtgcgt tttgaaagcc gtttgtataa
    38341 agatatttat cagaacaatt agtcaaaaag aaaccccggc acatggccgg ggttttgctg
    38401 attaacgctt gtaaatgaac tcaacgcctt cttcgtcgtc ttcgtcccag tcatcatccc
    38461 agtcctcatc atcctcttca gcaatctctt caagctgctg gcgatgataa tcatcccaca
    38521 tgaattcgac tttctctggc tgtttcgctt cttcagcctg cacgaccggg ttttcaatga
    38581 taaaggtcat cacatcccag cagagatctt tcacgcccag tccactcgcc gcagagatca
    38641 gataatattt atcttcccag cccagcgctt cagcaatcgc tttcgctttc tcttcagctt
    38701 ctgccttatc cagcagatcg atcttattga acactaacca acgcggtttc gccgccagat
    38761 cctggctgta tttttccagc tcgctgataa taatacgcgc gttttcaacc ggatcggtgc
    38821 cgtcaatcgg atcgatatcg atgaggtgca acaggacgcg gcaacgttcc aggtgcttca
    38881 ggaagcggat acccagacct gcgccttccg cagcgccttc aatcagtcct ggaatatcgg
    38941 caacaacgaa gctcttttcg ttgtccattc gtaccacacc cagacttggc accagagtgg
    39001 taaacggata atccgccact ttcggtttag ccgccgatac cgcgcgaata aaggtcgatt
    39061 tacccgcgtt tggcatcccc aacataccga cgtcagccag cagcatcagc tccagcagca
    39121 gctcacgctt atcacccggc gtaccgttgg ttttctgccg tggtgtacgg ttaacggacg
    39181 atttgaaacg ggtattgccc agaccgtgcc agccgccctt agcaaccagc agacgctgac
    39241 cgtgtttagt catatcgccc atggtttcac cagtaccctg gtcgattaca cgcgtaccta
    39301 ccggcacttt aatcgtcacg tctttaccgc gcttaccggt acaatcgcgg cttgcgccgt
    39361 tctgaccgcg ctctgcacgg aaagattttt caaaacgata atcgataagc gtgttcaggt
    39421 tttcgtcggc ttccatccat acgtcaccac catcaccacc gtcgccgcca tccgggccgc
    39481 ctttcggaat atacttttcg cggcggaagc tcacgcaacc attaccgcca tcacctgcaa
    39541 cgaccagaat cgatgcttca tcaacaaact tcattttatt ctccgtaaat cattcgcctg
    39601 cgcggggttg cgataccacc gttgtatgct tacgtaatcc gccccaaata cgatgaccaa
    39661 tggcggaata catcgcgccc gcaaccacga caaacgcacc gagataacct aaaaggttta
    39721 acatcggtct ggcgaagaaa tcgggccagg ccagtgataa aagatctgaa aaaaacagcg
    39781 taaacagtgg ggtgagcgtg atgatcgcgc tcacctgcgc tgcctgccag cgagccatcg
    39841 cttccgccag ggcgccatat cctaccaatg tattcagtcc gcaaaaaatt aaacatgcga
    39901 gctgccagtg gctaagctgc gctatcactc ctggctttgc cagagggaag agcgcaattg
    39961 tacataaagt gtacagcaaa aacaggatct gcggtgaggc cagccgacgc aataaaacct
    40021 tttgcgccac gccataactc acccaaaccg tcgccgcacc gaccccaaag atgactcccc
    40081 aggtgtaatc agtgagcttt gtaaatatct cgaccagact ggtgttaaaa aacatcacca
    40141 ggccgctcag gagcatcaat gcccctacaa cctgagtgct gcgcattttc tctttcagga
    40201 taaatacgct ggcaaccatc atgccaactg gcgagagttg cccaatcacc tgcgaagcgg
    40261 tcggactcag gtattgcaag gatgagctga acaggatgaa gttcccaaac agcccggcgg
    40321 tcgccactgc caacaaaatc aaccagcgtg gcttacgaaa cacgcgtaat ggcggcaacc
    40381 tcttcttcac cgcaagaatg gcacccaggc caatactcgc catcaagaaa cggtaaaaca
    40441 cgattgtcgg aggttccatc acctccagca cctgcttcat tgcgattggc aacgcccccc
    40501 agcaaattgc tgtggtgagc gccaaaagaa tgccaatgcc tgcctgctgc ttcataccgt
    40561 attccctgca aagcgcattt gcggattaac gccgcaccat gctcattttt accggttatc
    40621 gaatgtaaaa agccccgcaa cgtgttgcgg ggctttcatc cgttaccggg acgcgaaaaa
    40681 cttattcagc ttcgatgctg ataaatttac ggtttttcgg gcctttaact tcgaatttca
    40741 ctttaccgtc tgctttagca aacagagtgt ggtcacgacc gcaacctacg ttagcgccag
    40801 cgtggaattt ggtaccacgt tgacgaacga tgatgctacc cgccagaacg gattcgccac
    40861 cgaaacgctt aacgcccagg cgtttagctt ctgaatcgcg accgttacgt gtggagccgc
    40921 cagccttttt atgtgccatt tgaaatctct cctcaggtct taggcgctga tgccagtaat
    40981 tttcacatca gtgaaccact gacgatggcc ctgctgctta cgatagtgtt tacgacgacg
    41041 aaacttaacg attttaactt tctcgccacg accgtgagca acaacttcag ctttgattac
    41101 gccgccatca acgaaaggaa cgccgatttt gacttcttca ccgtttgcga tcatcagcac
    41161 ttcagcgaac tcaacagttt cgccagttgc gatgtccagc ttttccaggc gaacggtctg
    41221 accttcgctt actcggtgtt gtttaccacc actttggaaa accgcgtaca tataaaactc
    41281 cgcttccgcg cacacctttt caatgattca gagtgcgcta taaatattca caatagggcg
    41341 cgaatattac gcaaaacgca cgcctttgac aagtgctaca gtcaatacac gaagaaaaaa
    41401 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatc accctatatt
    41461 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    41521 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    41581 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    41641 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    41701 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    41761 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    41821 accgccaacg ctgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    41881 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    41941 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    42001 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    42061 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    42121 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    42181 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    42241 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    42301 gccatcgaac agggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    42361 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    42421 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    42481 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    42541 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaaag ggcgatgaat
    42601 ctttatatat tctgaatatt cacactcttt acaggaactt tttagagcaa taggccatca
    42661 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    42721 gaggaaagat ggaaagtaat ttcattgact ggcatccccc tgacatcatt gcaggtttgc
    42781 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    42841 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    42901 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    42961 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    43021 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgcttaatat
    43081 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    43141 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    43201 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    43261 cggtattgct ttcgatttcg gcatgcgcgc ccatacggct aagctccggc acatgcataa
    43321 agcggttttc aaagaccgtt tcggtgatga atccggtccc ttctgccacc aggttcaaca
    43381 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    43441 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    43501 ccgctccagc gtcacgcagt ttcgccagca cggcgtcgag agtatctggc tgcgcgttac
    43561 ggcagataat tttgccgcga gaaatcgccg ccgccaccag gaaagtaccg gtttcgatac
    43621 gatccggtag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    43681 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    43741 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt accttccgcc agggttgcag
    43801 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    43861 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    43921 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    43981 gcagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagtgcc cagatagaag
    44041 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    44101 tatgcacaga accattacgt tctactttcg cacccagctg acttagcagc ttcattgatg
    44161 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagag
    44221 cggcaaaaag gataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    44281 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    44341 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    44401 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    44461 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcatgccg tcaaacaact
    44521 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    44581 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    44641 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    44701 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    44761 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    44821 tcgccagggt atacactttg tcgtttaccc cctgaagcgt cacattgttg ccctgctttt
    44881 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    44941 ggctaagatc gatgcaggta atccccttca ccgcttcctc acgcatttcc caaagcggta
    45001 gcaaaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    45061 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    45121 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    45181 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcctgcc agttgcccgt
    45241 ctgggagttt ttacgccact ggaagtccag acgcaccggc gggcggccat tcggatcaat
    45301 aatggtaacg cgaatcggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    45361 ataggtttga ccatgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    45421 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    45481 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    45541 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    45601 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    45661 actcagcggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    45721 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    45781 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    45841 aatgagatct tccagcacca tcgcagactt agtgtcctga attgtatcgc catccttcag
    45901 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttcccc
    45961 cagtaggccg gaagtacgaa tgctcagcga actggtatct ggaatgtggt tataacgttg
    46021 ttcaatttcc agcgttacgc gtggcagata ggttttcggg tccagcgtaa tatccgccac
    46081 ccggcccaca acaacgccac caatactgac cggagagcgc gctttcaggc cgccaatgtt
    46141 atcgaacgtc gcataaagcg tgtaggtcgg ttcagtacgt atggacgtca cgttcgccgc
    46201 cttcaggcaa acaaacagag ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    46261 ttcatttttt ttcgtttgca tgtactcaat tcccaaacat caatgcggtc agcacaaaat
    46321 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    46381 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    46441 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    46501 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagagatc
    46561 cgccccagat ccccacggcg acgaaaataa ccgtcaacag tggtaatgaa ataaccccag
    46621 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    46681 agagttgctc tgtagcgcgc atcaggccga tttctgcggt tagcgccgaa ccagcacgcc
    46741 cggcaaacaa caacgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    46801 gcatacccag actggtttcc gcactataag tggtcagaac cagataacct tgcagcccca
    46861 acaccattcc gatgaacacg ccagaaacca caataatcag catcgacagg acgccgacat
    46921 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    46981 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    47041 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    47101 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    47161 tgacgtacgc gcggatcagg attcgcctgc aacgcctggg cacagccatg agcgacaatt
    47221 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    47281 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    47341 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    47401 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    47461 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    47521 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    47581 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttgcgcact
    47641 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    47701 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    47761 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    47821 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    47881 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    47941 aacccaacat atttacagaa tattacccgc tgtggttagc gaaagctggc atttgtttta
    48001 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    48061 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    48121 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    48181 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    48241 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    48301 cgttggtaca gccctcggct caaacattat caatattttg ctgatcctcg gtctggctgc
    48361 gctggttcgt ccttttaccg tccattccga tgttctgcgt cgtgaattac ccttaatgtt
    48421 gttggtcagc gtagtggccg gttccgtact ctatgacgga caacttagtc gcagcgatgg
    48481 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    48541 ggctgaacgt caggggactg acagcctgac cagagagcag cttgcagagc tgccgcgtga
    48601 cggcggattg cccgtcgcgt ttttatggct cggcattgcg cttatcatca tgccagtggc
    48661 cacgcggatg gtggttgata acgccacggt gctggcgaat tactttgcca tcagcgagtt
    48721 gacgatgggt ctgacggcaa ttgctatcgg aaccagcctg ccggaactgg caaccgcaat
    48781 agcgggggtt cgcaaaggtg aaaacgacat tgctgtcgga aatatcattg gcgcaaacat
    48841 ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata acaccaggag agattgatcc
    48901 attggcgtac agtcgtgact acagcgtgat gttgctggtg agcattattt ttgcgttgct
    48961 gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg gtattattaa ctggcggatt
    49021 tatcgtatgg ctggcgatgt tgtactggtt atcgccaata ctcgttgaat aactggaaac
    49081 gcattatgtc gcacgtagag ttacaaccgg gttttgactt tcagcaagca ggtaaagaag
    49141 tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca atacatcaat cagaatttca
    49201 cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt tgtcgtcatg gggatgggga
    49261 aatcggggca catcgggcgc aaaatggccg caacgtttgc cagcaccggt acaccttcat
    49321 ttttcgtcca tcctggtgaa gccgcgcatg gtgatttagg catggtcacc ccacaggatg
    49381 tggtgattgc tatctctaac tcaggtgaat ccagcgaaat cacggcctta attccagtgc
    49441 ttaagcgtct tcacgtaccg ttaatctgca tcaccggtcg cccggagagc agcatggcgc
    49501 gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga agcctgtccg ttagggctgg
    49561 caccgaccag cagcaccacc gccacgctgg ttatgggcga tgccctcgct gtcgcgctgt
    49621 taaaagcacg cggctttact gctgaagatt ttgcgctctc acacccaggc ggcgcactgg
    49681 gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac gggcgatgag atcccgcatg
    49741 ttaagaaaac ggccagtctg cgtgacgcat tgctggaagt tacccgcaaa aatcttggta
    49801 tgactgtcat ttgcgatgac aatatgatga ttgaaggcat ctttaccgac ggtgatttac
    49861 gccgtgtctt cgatatggga gtggatgttc gtcagttaag tattgccgat gtgatgacgc
    49921 cggggggaat acgcgtgcgc cctggcattc tggccgttga ggcactgaac ttaatgcagt
    49981 cccgccatat cacctccgtg atggttgccg atggcgacca tttactcggt gtgttacata
    50041 tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa ggataaacaa caatgagcaa
    50101 agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc gccgacgtta tagcaaaagc
    50161 agagaacatt cgtctgctga tcctcgacgt tgatggcgta ctgtcagatg gcctgattta
    50221 tatgggcaat aacggcgaag agctgaaagc gttcaatgtt cgtgacggtt atggcattcg
    50281 ttgtgcgctc acctctgata ttgaagtcgc tatcattacc gggcgaaagg ctaaactggt
    50341 agaagatcgt tgtgccacat tggggatcac tcacttgtat caggggcagt caaacaaact
    50401 gatcgccttt agcgaactgc tggaaaaact ggcgattgcc ccggaaaatg tggcttatgt
    50461 cggcgatgat ctcatcgact ggccggtaat ggaaaaagtg ggtttaagcg tcgccgtggc
    50521 cgatgctcat ccactgttga tcccgcgcgc cgattatgtg acgcgcattg ctggcggtcg
    50581 tggcgcagtg cgagaagttt gcgacttatt actcctggcg cagggcaaac tggatgaagc
    50641 caaagggcaa tcgatatgag taaagccaga cgttgggtta ccattgtgct atcactggcg
    50701 gttctggtga tgatcggcat taatatggcc gaaaaagacg ataccgccca ggtggtcgtc
    50761 aacaacaatg atcccaccta taaaagcgag catacggaca cgctcgtcta taacccagaa
    50821 ggggcactaa gctatcgatt gattgctcaa cacgttgaat attattccga tcaggccgtt
    50881 tcgtggttta cgcagccggt acttaccacg tttgataagg ataaaatccc gacatggtcc
    50941 gtaaaagcag ataaagccaa gctgaccaat gaccggatgc tctatttata tggacacgtt
    51001 gaagtcaacg cacttgtgcc ggactctcaa cttcgcagaa tcacgacgga taacgcgcag
    51061 atcaatctgg tgacgcagga tgttacctct gaagacctcg tcacgttata cggaacaaca
    51121 tttaactcca gcggtctgaa aatgcgcggc aacttacgca gcaagaacgc cgagctgatt
    51181 gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa ctcagcctta atcttgtgct
    51241 tgccagctca cttctggccg ccagcattcc ggcatttgcc gtaaccggag acactgatca
    51301 gccgatccac attgaatcgg accagcaatc tcttgatatg caaggcaacg tggttacctt
    51361 taccggtaat gtcatcgtca cccagggcac catcaaaatt aatgccgaca aagtggtcgt
    51421 tacccgtccg ggcggcgaac aaggtaaaga agtgattgac ggctacggta aaccggcaac
    51481 gttctaccag atgcaggaca acggtaaacc cgttgaaggt catgcttccc agatgcacta
    51541 cgaactggca aaagattttg tcgttctgac gggtaatgct tatctgcagc aggtcgatag
    51601 caacattaag ggcgataaga tcacttacct ggtgaaagag cagaaaatgc aggctttcag
    51661 cgacaaaggc aagcgcgtaa caaccgttct ggtgccgtcg cagctgcagg acaaaaacaa
    51721 caaaggccag accccggcac agaagaaggg taattaattc gttatggcaa cattaactgc
    51781 aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta gaagacgtca gcctgaccgt
    51841 caactccggg gaaattgtcg gtctgctggg gccaaacggt gccggtaaga ccaccacttt
    51901 ctatatggtt gttggcattg tgccgcgcga tgcgggcaac atcattattg atgatgacga
    51961 tatcagtctg ctgcctctgc atgcacgcgc gcgccgcggt atcggctatc tgccacagga
    52021 agcctccatt ttccgtcgcc tcagcgttta cgataacctg atggcggtac tgcaaattcg
    52081 tgacgacttg tctgctgaac aacgtgaaga ccgcgcgaac gagctgatgg aagagtttca
    52141 cattgagcac ctgcgtgaca gcatggggca gtcactctcc gggggtgaac gtcgccgtgt
    52201 agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt ctactcgacg aaccgtttgc
    52261 cggggttgac ccgatctcgg ttatcgacat taaacgcatc attgaacacc tgcgcgacag
    52321 cggcctgggc gtactgatca ctgaccacaa cgtgcgtgaa acactggcgg tttgtgaacg
    52381 cgcttatatc gtcagtcagg ggcatttgat tgcccacggc acgcctacag aaatcttaca
    52441 agacgaacac gttaagcgtg tataccttgg ggaagacttc agactctgat agggtagaag
    52501 tttgcgacgt tttagcagga gagtacgatt ctgaacatga agcaaggttt gcaactcagg
    52561 cttagccaac aactggcgat gacgccacag cttcaacagg cgattcgtct gttgcagttg
    52621 tcgacgctgg aacttcagca ggagctacag caggcgctgg agagtaatcc gctgcttgag
    52681 caaatcgaca ctcatgaaga aatcgacacc agcgaaacgc aagacagtga aacgctggac
    52741 accgccgacg cgctcgaaca aaaagagatg ccggaagagc tgccgctcga tgccagttgg
    52801 gacaccattt acaccgctgg tacaccatcc ggcaccagcg gtgactacat tgacgatgag
    52861 ctgccggtct atcagggcga aacgacgcag accttgcagg attacctgat gtggcaggtc
    52921 gagctgacac cgttttccga cactgaccgc gctattgcta cctctatcgt cgatgccgtt
    52981 gatgacaccg gttatctgac tgtcccgctg gaagatattc tcgaaagtat gggcgatgaa
    53041 gagatcgaca tcgacgaggt tgaagccgtc cttaagcgga tccaacggtt tgatccggtc
    53101 ggtgtagcgg caaaagatct gcgtgactgc ctgctaatcc aactctccca attcgataaa
    53161 accacgccgt ggctggaaga ggccagactg atcattagcg atcatctcga tctgttagcc
    53221 aatcacgact tccgcacttt aatgcgcgtc acgcgtctga aagaagatgt gctgaaagaa
    53281 gccgtcaatc tgatccagtc gctcgatccg cgccccgggc agtcgatcca gactggcgaa
    53341 cctgagtatg tcattccaga tgtgctggtg cgtaagcata acggtcactg gacggtagaa
    53401 ctcaacagtg acagcattcc gcgtctgcaa atcaaccagc actacgcctc gatgtgcaat
    53461 aacgcgcgca acgatggtga cagccagttt atccgcagca atctgcagga tgccaaatgg
    53521 ttgatcaaga gtctggaaag ccgtaacgat acgctactgc gcgtgagtcg ctgtatcgtt
    53581 gaacagcagc aagccttctt tgagcaaggt gaagaatata tgaaaccgat ggtactggcc
    53641 gatatcgccc aggctgtcga aatgcatgaa tcgacgatat ctcgcgtgac cacgcaaaaa
    53701 tacctgcata gtccacgagg catttttgaa ctgaagtatt tcttttccag tcacgtcaat
    53761 accgagggcg gcggcgaagc ttcctccacg gcgattcgtg cgctggtgaa gaaattaatc
    53821 gcggcggaaa acccagcgaa accgttgagc gacagcaagt taacctcttt gctgtcggaa
    53881 caaggtatca tggtggcacg ccgcactgtt gcgaagtacc gagagtcttt atccattccg
    53941 ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat aaggaagaca ctatgcagct
    54001 caacattacc ggaaataacg tcgagatcac cgaggcactg cgcgaatttg ttagagccaa
    54061 atttgccaaa cttgagcaat attttgaccg aatcaaccag gtctatgttg ttctgaaagt
    54121 ggagaaagtc acccacacct cagatgcaac actgcatgta aacggtggtg aaattcatgc
    54181 cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc ttaattgata agctggcacg
    54241 ccagttgacc aaacataaag ataaactgaa acaacactaa ttgtccgggc aattagcatg
    54301 tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta cggttaatgc tccgagcctg
    54361 ttccactgtt tgagtgggca ggttcttagg tgaaattatg acaaataatg atacaactct
    54421 acagcttagc agtgttctta acagggaatg tacgcgaagc cgcgttcact gtcagagcaa
    54481 aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa caacttagcc tgccgcctca
    54541 ggtggttttt gaagctatcc tgacgcggga aaaaatgggc agtaccggta tcggcaatgg
    54601 tattgccatt ccgcatggca aactggaaga agatactctg cgcgccgttg gcgttttcgt
    54661 tcagctcgaa acgcctatag ctttcgatgc catcgacaac caaccggtgg acttactttt
    54721 tgccctgctg gtgccggcag accaaactaa aacgcacctg catactctgt cgctggtggc
    54781 gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc gcagcccaga gcgatgaaga
    54841 gctgtatcaa atcattacgg ataccgaagg tactccggat gaagcgtagt tattcggtaa
    54901 tgtctctttt agacgttgtg aggagaaaca gtacatggta ctgatgatcg tcagcggacg
    54961 ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa gatatgggtt tttactgcgt
    55021 ggataacctt cccgtagtgt tgttacccga tctggctcga accctggccg atcgcgagat
    55081 ttctgccgcc gtcagcattg atgttcgtaa tatgccggag tcaccagaaa tattcgaaca
    55141 ggcgatgagt aacctgcctg acgctttctc accgcaactc ctgttcctgg atgccgaccg
    55201 taatacctta attcgtcgtt acagtgacac gcgccgactg catccgcttt ccagcaaaaa
    55261 cctgtcgctg gaaagtgcta tcgacaaaga aagcgatttg ctggagcctc tgcgttcgcg
    55321 agcggatctg attgtcgata cctcagaaat gtccgttcac gagctggctg aaatgctgcg
    55381 tacccgtctg ctgggtaaac gtgaacgcga actgaccatg gtctttgagt ctttcggctt
    55441 caaacacggt atccctatcg atgcagatta cgtctttgac gtgcgcttct tgccgaaccc
    55501 gcactgggat ccgaaactgc gtccaatgac aggtcttgat aaacctgtcg ccgcgttcct
    55561 cgaccgccac acagaagtac acaattttat ctaccagacg cgaagctatc ttgagctatg
    55621 gttacctatg ctggaaacca acaaccgtag ctacttgacg gtcgccattg gttgtaccgg
    55681 cgggaagcac cgttcggtgt atattgcaga gcaactggca gactacttcc gctcgcgcgg
    55741 taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt aaaccatgac cgtcaagcaa
    55801 actgttgaaa tcacaaacaa gctgggcatg catgcccggc ctgcaatgaa gctgtttgaa
    55861 ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg acgaaggcac cgaggccgaa
    55921 gccaacagcg ttattgcgct gctgatgttg gattctgcca aaggacggca gattgaagtt
    55981 gaagcgaccg gcccacagga agaggaagca ctggccgccg ttatcgccct ctttaactct
    56041 ggttttgatg aagattaatc ttcatcactt tgacatacaa ctcccttcaa actccccccg
    56101 acaataagaa aatcacgtac tgaaatcgtt ctcaatcaac gtcatttgta cattttgtgc
    56161 gcttttcact ttcagaagaa ccttaagaaa accttaagag gcattgttta ggttttgttt
    56221 aagttaatcg accatactgg agatcgtcag aaaatatttc caggagatgg catgattcgc
    56281 ttatctgaac aaagtcccct gggcaccgga agacatcgga aatgttatgc gcatccggaa
    56341 gatgcccaac gctgtatcaa gattgtctac catcgtggcg atggtggcga taaagagatc
    56401 cgccgtgagt taaagtacta cgcgtatctt ggtcgccggt taaaagactg gagtggaata
    56461 ccgcgctatc acggtaccgt tgagacggat tgcggaaccg gttatgtcta cgatgtgatt
    56521 gcagattttg acggcaaacc ctccattacg ctgaccgaat ttgctgaaca atgccgttac
    56581 gaagaagaca ttgcacaact gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat
    56641 aaccgtatcg tgacgatgtc gctgaagccg cagaatatcc tctgtcacca catcagtgag
    56701 tcagaggtta tcccggtcgt ctgcgacaac attggtgaaa gcacattgat tccactggcg
    56761 acctggtcga agtggtgttg tctgcgtaag caagagcggt tatggaaacg atttatcgcc
    56821 caaccggcgc tggctattgc cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg
    56881 gccctcactt cgcgcgaagc ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct
    56941 aactgataca tctgccgtaa aatccacgcc tgacggctac gcacgtagcc cgatggcgag
    57001 gagactttga aacgaagtgg attaggtaat acagctgcca gtaatgcagc ttccgaccgg
    57061 gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg cagcttcgac gccaaacaca
    57121 ccgtcgccaa attcggcgat attcaggtaa accgtcagga tacgcttttt gctccagact
    57181 gtttctatcc ccagcgttaa tccagcttcc agcccttttc gtacccagct acgcccatcc
    57241 cataaaaaga gatttttggc cgtctgctga gaaatcgttg aagcaccgcg aatacggttt
    57301 tcattgcgct cgttgtgcgc cagggctttc tcaatggaag cgacatcaaa gccccggtgc
    57361 tcaggaaatt tctgatcttc tgcggcaatc accgccagtc ccatccacgg cgagatttga
    57421 tccatactga cccagtcaga atgtgccacg taacgaaaat tgccatgcag ccaggcgctg
    57481 acctgtcgct cgaccattac cgctgagaag ggaacaggcg caacactaaa caacgcgatg
    57541 cccccgcccc agaaaacggc gaggacaacc attaaccgca aaagaaaacg gcgaacgaaa
    57601 ctaaacaccg ttaagcggct tttactcatt cagccagaac cagcacgcgg gaaaccagct
    57661 tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag catatatgct ggggtggtga
    57721 caatcttatt gtcttcatca accacgatat catcgacagg acagggcaca tgctccgcgc
    57781 ccatctcttc cagcacttct gcggtatcga tatcagtacc gatggtcaaa cgcagcggga
    57841 aatcgaaaat tttcggcagc atcgccgggg caatacacat aaaaccaagc ggttttccgg
    57901 cctgatgcat cgcttgtgcc agcgccttta attcacggtc aacggtgcat tcgctaccaa
    57961 gagtggcaaa attgcttaaa ttcttcgccg cgccaaaccc ccccggcaca atcaacgcat
    58021 ccagttcagc ggcatcggcc tgggccagag gacggatttc accgcgcgtt attcgtgccg
    58081 cttcaatcag cacgttgcgc gtttccgtca tcgcttcgcc agttaaatgg ttgataacat
    58141 caacctgctg cttatccggt gcaaagcaga ccgcctgcgc accgctgcgt gaaatagcta
    58201 acaacgtcaa caccgcttca tgaatttcag aaccgtcata gacgccgcat ccgctcagaa
    58261 ttacgccaat tttcttcatt gtgatcatcc tttcgcaatt gactgaaaca cattaatttt
    58321 tttaataaaa atggtacgca tcacacattt aactgattca tgtaacaaat catttaagtt
    58381 ttgctatctt aactgcgtgc ggcctgaaaa acagtgctgt gcccttgtaa ctcatcataa
    58441 taatttacgg cgcagccaag atttccctgg tgttggcgca gtattcgcgc accccggtct
    58501 agccggggtc attttttagt ggcttttgcc acccacgctt tcagcacttc tacgtcgtga
    58561 cgccactctt ctttcatctc ttcaatccat tcaccgacgt tatcttccca ggcaggaagg
    58621 tcaggagact gaatttgctg acccagctgt tgcagatggc gtaaccccac tgaccccgcc
    58681 gcacctttaa ttttatgtcc ttcctcaaca atgccttttt tatcctgcgc cgtcagattc
    58741 gactccagca cgctgacata gcccggcatc atcttctcaa acaccgctaa cccgtcggtg
    58801 atcagcttcg gtcctacaag ttcgagatac tgttccagca tgggaatatc gagcaatgct
    58861 tctgatttac tgttctcttc tgtcgtcacc gtactctcct catcatcctg ggtatcccag
    58921 aatttcttga tcatcgcggt tagcgccgga accgaaagcg gcttactcag cacatcatcc
    58981 attccagcat tgaggtactc ttgtttgtct ttcagcacgt tagcggttaa ggccaccagc
    59041 ggcggtaaat cctcgcgcgg ataacgcttc gtcagttcac gagagatatc cagcccggtc
    59101 atatctggca actgaatatc cagcaacacc aggtcgtatt cgcccggttt aaacatctcc
    59161 agcgccgcct tgccggtcat ggcgacatca acgctgttac ctaatttttc cagcacagaa
    59221 cgcgcaacaa tcacgttcag ttcaatgtct tccaccagca gcacattcag cgccggtaaa
    59281 ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg ctaccgacgg tgcgtggatc
    59341 gtcaacgtaa aggttgaacc tttgccctgt tcgctggtaa ccgtaatatc gccgcccata
    59401 tttttcgcca gacgacgaga aacggccaga ccaataccgg tgccggtggc aggtttaccg
    59461 ccatgactgt ctttcacctg gtaatacatg gcgaaaattt tatccagctc atcctgcgga
    59521 atgccgatac cagagtcttc cacttcaaaa tgcagcatat cgccttcatc gtagcgcacg
    59581 cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt tactgatgag gttccacagg
    59641 atctgccgta aacgcgtccc gtcggtaatg acctgatgcg gtaatggcag cgtcggctcc
    59701 aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg agagattttc cagatcggca
    59761 aggaagctgg tgaaatcaac cggttgatta tcaagctgga ccttgcgccg ttccatctta
    59821 tccatgtcga taatatcgtt aaagatattc cccagcgtga cggccgaaac atggatggtc
    59881 ttgagatatt tttcctgctc ggcggtgagt tcggtatcca gcagaatgcg gctcagaccg
    59941 acgataccgt tcagcggtgt acgcaattcg tgactgatgg tggagataaa cgtcgttttg
    60001 tcgcggctgg cccgttcaag cgcatcctga taccgcttac gctcggtaat gtcgcgacca
    60061 aagcccatca aaccgtgacg tttacccacg cggtcgtagt acggcacttt acggatttca
    60121 aagcaggctt tgcgcccgtc cgggtaatcc agccactgtt cataggtcag tgacacatta
    60181 tgacggaaca ctttttcatc ggtttcaatg acttttgcgg cggcttccgg tgagtaaaca
    60241 tcagcaggtt tcaggtgaac cagttgtttt tcgctttttc cggtcagcag ctccatcgcg
    60301 cggttacagc cggaaaactc tttatcttcg ttacgataaa aaaccaggtc gggtgaagca
    60361 tcaaggaagg aacgtaagaa tgaggattgc tgctcgagct gaatttgtgt ctcttcgcgc
    60421 tctttgattt caattttcag ttggccgaag gtttcctgca gttctgcttc cgctttttca
    60481 cgaacggcaa tttcctgatt tagctgggca atattatctt ttaactgaac gttgaggctc
    60541 aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc gtgacagacg ttgtcgtgac
    60601 tcctccagtt gctcgacgac caccgataga aagtagaccg cccacggcgt aatcagcaaa
    60661 ccaaagaaga tagaacgaat aacatcaatg ctttcgacct gaccatgcag caccatggtt
    60721 accgccattt gtaccacaat ggcaagaacg acgagggcca gcgccagcaa cattgagaag
    60781 cgcaccagac ctaacttcat catcaggtca acataatact gcgccagcag acgaatttgc
    60841 ttcattaggg aattccttca cgacaacctg ccccaataat acccaattct gacagttacg
    60901 ttataaattg tgtgagaaat gcggaattcc cctcaacgac ctactccgtt ggaggtagcc
    60961 acggacgtcc cagcgccgat ccctgtacgc catgttcatt cagatagcga tccagctcga
    61021 ccattcccgt ccatcgattt tcgcaccaca gcggagcaag cagcgtcggg cgacgggcgc
    61081 tggcggaaat gcggtggtag atcacttccg gtggcgtatg gcgaatcatt tctccggcag
    61141 tgagcgtgta atcctccagt tcaataccgt tcaaacgccc cgcttcccag gctttcgcca
    61201 taatgctgcc tttcacaata tgcagcggat gcagctttat gccgtccacg ccggtttcaa
    61261 ccactcgctc cagcgtttgc aaacattcag cctgcccttc accaggcagg ccgacaatta
    61321 agtgggaaca caccttcagc ccgcgctgac gtgccagctg ggttgtacgc tgataacagg
    61381 caaaatcatg gccgcggttg atgcgatgca gtgttttgtc gtgggcggtt tgtagcccca
    61441 gctccagcca cacttcgtaa ccctggtcct tatattcgca aagcagatcc agcaccgcat
    61501 ccggcacgca gtccgggcgg gtaccaacac acaaaccgac aatattggcc tggctcaccg
    61561 cctgctgata catagaacgc agcacctgaa cttccgcaaa ggtgctagta tacgcctgaa
    61621 agtaggccag atagcgttta gcgcggttaa ctaaattcgc ctggtgcgcc agttgctcgg
    61681 caatggaacg atgctgctgc gcttcatcgg caaacgaggc aacattacag aatgtgcagc
    61741 caccacgccc gatggtaccg tcacggttag ggcagctaaa accgccgtgc agcgtcagct
    61801 tatgcacctt ttgcccataa cgacgggtga gatcaccacc aaacatattg actaatttct
    61861 gtaactgcat aatctgatag accgcgcctt gaaaagaggc caaagcctgc catttttagc
    61921 tcaattcggc gatgacctgg atcaatcgtc ccggcctgct tttatcaact gcataatcaa
    61981 tcaaaattac cgaaatttca tgcataatca cataaatcac ttttgcttat cttgtgtcag
    62041 atttttttat ctccttatgg attttaggca aaaacagtag catgaaacgt cattaccaat
    62101 taaggcagta taaaatgctg gttttctcgt cagttcaagg taggataagg gttaacacgc
    62161 ctttatgaca gtcaggaatt gactgtttct ctaacgactt cccttttagc cttaaagata
    62221 aaattcattt taatttcagt tgcttaataa agaattttgc gctaaagcac atttctgtac
    62281 caataagctt gccatttgac ctgtatcagc tttcccgata agttggaaat ccgctggaag
    62341 ctttctggat gagcagcctg ctcatcatat ttatgcagta attgagatcc cctcttcacc
    62401 gtattaaccg atgcgaaaag gacaacaagg gggcgaatac gaggcgcgcg tatgacacgc
    62461 aacccccgtc gccacgctct ttctgtgccc gtgcgcagcg gttcggaagt ggggttcccg
    62521 cagagcctgg gggaggttca cgatatgttg tacgataaat cccttgagag ggataactgt
    62581 ggtttcggcc tgatcgccca catagaaggc gaacctagcc acaaggtagt gcgtactgca
    62641 atacacgcac tggcccgcat gcagcaccgt ggcgcgattc tcgccgatgg taaaaccggt
    62701 gacggttgcg gcttgctgtt acaaaaaccg gatcgctttt ttcgcatcgt tgcgcaggag
    62761 cgcggctggc gtttagcaaa aaactacgct gtcgggatgc tcttcctgaa taaagatcct
    62821 gaactcgcgg ctgccgcacg ccgcatcgtt gaagaagagc tgcaacgcga aaccttgtcg
    62881 attgtgggct ggcgtgatgt ccccactaac gaaggcgtgc tgggtgaaat cgccctctcc
    62941 tctctgcccc gcattgagca aattttcgtt aacgctccgg caggctggcg tccgcgggat
    63001 atggagcgcc gtctgtttat cgcccgccgc cgcattgaaa agcgtctcga agccgacaaa
    63061 gacttctacg tctgtagcct gtcgaatctg gtgaacatct ataaaggtct gtgtatgccg
    63121 gcggatctgc cgcgctttta tctggatctt gcggacctgc gtctggaatc ggccatctgc
    63181 ctgttccacc agcgcttctc cactaacacc gtaccgcgct ggccgctggc gcagccgttc
    63241 cgctacctgg cgcataacgg tgaaatcaac accatcaccg gtaaccgcca atgggcgcgt
    63301 gcgcgtacct ataaattcca gacgccgctt atccctgatc tgcacgacgc cgcaccgttc
    63361 gtcaacgaaa ccggctctga ctccagttcg atggataaca tgctggaact gctgctggca
    63421 ggcgggatgg atatcatccg cgccatgcgt ctgttagtgc cacccgcctg gcagaacaac
    63481 ccggatatgg acccggaact gcgtgccttc tttgacttta actccatgca tatggagccg
    63541 tgggatggcc cggcgggcat cgtgatgtcc gacggtcgtt ttgccgcctg taacctcgac
    63601 cgtaacggtc tgcgtccggc gcgctacgtc atcaccaaag ataagctcat cacctgcgcc
    63661 tctgaagtcg gtatctggga ttaccagcct gacgaagtgg tcgaaaaagg ccgcgtcggg
    63721 ccaggcgaac tgatggttat cgacacccgc agtgggcgta ttctgcactc ggcagaaacc
    63781 gatgacgatc tgaaaagccg ccatccatat aaagagtgga tggagaaaaa cgtccgcaga
    63841 ctggtaccgt ttgaagatct gcccgatgaa gaagtgggta gccgcgaatt ggacgacgac
    63901 acgcttgcca gctaccagaa acagtttaac tacagtgcgg aagagctgga ctccgtaatt
    63961 cgcgtactgg gcgaaaacgg tcaggaagcg gtcggttcga tgggcgatga taccccattc
    64021 gccgtgctct ccagtcagcc gcgcattatt tacgactact tccgccagca gtttgcccag
    64081 gtgactaacc cgccaatcga cccgctgcgt gaagcgcatg ttatgtcgct cgccaccagt
    64141 atcggtcgtg aaatgaacgt cttttgcgaa gcagagggcc aggcgcaccg tttaagcttt
    64201 aaatcgccga ttctgctcta ctccgatttc aaacagctca cgacgatgaa agaggagcac
    64261 taccgcgcag atacgctgga tatcaccttt gacgtcacta aaaccacgct cgaagcgaca
    64321 gtcaaagagc tgtgcgacaa agccgaaaaa atggtacgta gcggcaccgt gctgctggtg
    64381 ctctccgacc ggaatatcgc taaagatcgc ctgccggttc cagccccgat ggcggttggc
    64441 gcgatccaga cccgtctggt cgatcaaagc ctgcgttgcg atgccaacat catcgtcgaa
    64501 accgccagcg cccgcgatcc gcaccacttc gccgtgttgc tgggcttcgg cgcgacggct
    64561 atttatccat accttgccta tgaaacgctg ggccgcctgg tagacaccca tgcgattgcc
    64621 aaagattatc gtaccgtgat gctcaactac cgtaacggca tcaacaaagg cttgtacaaa
    64681 atcatgtcca aaatgggcat ctccaccatc gcctcttacc gctgctcgaa actgtttgaa
    64741 gcggtcggtc tgcacgatga tgtagtgggc ctgtgcttcc agggggcggt cagccgcatt
    64801 ggtggggcga gctttgaaga cttccagcag gatctgctga acctgtcgaa acgtgcctgg
    64861 ctggcgcgta agcccatcag ccagggcggc ctgctgaaat acgtccacgg cggcgaatac
    64921 cacgcttaca acccggacgt ggtgcgcacg ctgcaacagg cggtacaaag cggcgagtac
    64981 agcgactatc aggaatacgc gaagctggtt aatgagcgtc cggcaaccac gctgcgcgat
    65041 ctgctggcaa ttacgccggg tgaaaacgcg gtcaacattg ctgatgttga accggcaagc
    65101 gaactgttta aacgctttga taccgccgcg atgtctatcg gcgcgttaag cccggaagcc
    65161 cacgaggcgc tggcggaagc gatgaatagc atcggcggta actcgaactc cggtgaaggc
    65221 ggcgaagacc cggcgcgcta tggcactaac aaagtgtcgc gcatcaagca ggtggcttct
    65281 ggtcgcttcg gcgttactcc ggcgtatctg gtcaatgccg acgtcattca gattaaagtc
    65341 gcccagggcg cgaagccggg cgaaggcggt cagttgccgg gcgacaaagt cactccttac
    65401 atcgccaaac tgcgctattc ggtgcccgga gtgacgctga tctccccgcc gccgcaccac
    65461 gatatctact ctatcgagga cttagcgcag ctcattttcg acctcaagca ggttaacccg
    65521 aaagcgatga tctccgtgaa gctggtttcc gaaccgggcg taggcaccat cgcgactggc
    65581 gtggcaaaag cttatgccga tttaatcacc atcgcaggct atgacggtgg caccggcgca
    65641 agcccgctgt cgtcagtgaa atacgcaggt tgcccgtggg agctggggct tgttgaaacc
    65701 cagcaggcgc tggttgccaa tggtctgcgt cataagatcc gtttgcaggt cgatggcggc
    65761 ctgaaaaccg gtgttgatat catcaaagcg gcgattctcg gcgcagaaag cttcggcttc
    65821 ggcactggcc caatggtcgc gctcggctgt aaatatctgc gtatttgcca cctgaacaac
    65881 tgcgcaacgg gtgtggcaac tcaggatgac aaactgcgta agaatcacta tcacggcctg
    65941 ccgttcaagg tgacgaatta ctttgagttt atcgcccgtg aaacccgcga gctgatggca
    66001 cagctggggg taacgcggct ggtggatctg attggtcgca ccgacctgct gaaagagctg
    66061 gacggtttca ccgccaaaca gcagaaactg gcgctgtcga agctgctgga gaccgccgaa
    66121 ccgcatccag gtaaggcact ctactgcacc gaaaacaacc cgccgtttga taacggcctg
    66181 ctgaacgcgc agttgctgca acaggcgaaa ccgtttgtcg atgaacgcca gagcaaaacc
    66241 ttctggttcg atatccgcaa taccgatcgc tccgtgggcg cgtcgctttc aggctatatc
    66301 gcccagacgc acggcgatca ggggctggca gccgatccta tcaaagcgta cttcaacggc
    66361 accgcaggcc agagtttcgg tgtatggaac gcgggcggcg tggaactgta cctgaccggg
    66421 gatgccaacg actatgtcgg taaaggcatg gcgggcggct taatcgccat tcgtcctccg
    66481 gttggttccg ccttccgcag ccatgaagca agtattatcg gcaacacctg cctgtatggc
    66541 gcgaccggtg gtcgtctgta tgccgcaggc cgcgcgggtg aacgtttcgg cgtgcggaac
    66601 tccggtgcta tcaccgtggt agaaggcatt ggcgacaacg gctgtgaata tatgacgggc
    66661 ggtattgtct gtattctggg taaaaccggc gttaacttcg gtgcgggcat gaccggcggc
    66721 ttcgcttacg ttctcgatga aagcggtgat ttccgcaaac gcgttaaccc ggaactggtc
    66781 gaggtcttaa acgttgacga tctggcgatc catgaagagc atctgcgcgg tcttatcacc
    66841 gagcatgttc agcatactgg ctctcagcgc ggtgaagaga ttctggcgaa ctggtcaacc
    66901 ttcgccacta aatttgcgct ggttaaaccg aagtccagtg atgtaaaagc actgctgggt
    66961 caccgtagtc gtagcgcagc agagttgcgc gtgcaggcgc agtaaggggt agcaacaatg
    67021 agtcagaatg tttatcaatt tatcgacctg cagcgcgttg atccgcctaa gaaaccgctg
    67081 aagatccgca aaattgagtt tgttgaaatt tacgagcagt tttccgaagg ccaggccaaa
    67141 gcgcaggctg accgctgcct gtcgtgcggc aacccatact gcgagtggaa atgtccggta
    67201 cacaactaca tcccgaactg gctgaagctc gccaacgagg ggcgtatttt tgaagcggcg
    67261 gaactgtcgc accagaccaa caccctgccg gaagtttgcg gacgagtctg cccgcaagac
    67321 cgtctgtgcg aaggttcctg cactctgaac gatgagtttg gcgcggtgac catcggcaac
    67381 attgagcgtt atatcaatga taaagcgttc gaaatgggct ggcgtccgga tatgtccggc
    67441 gtgaagcaaa ccggtaaaaa agtggcgatt atcggcgcag gcccggcagg tctggcgtgt
    67501 gcggatgtcc tgacgcgtaa cggcgtaaaa gccgttgtct tcgaccgtca tccagaaatt
    67561 ggcgggctgc tgaccttcgg tattccggcc ttcaagctgg aaaaagaggt aatgacgcgt
    67621 cgccgtgaaa tcttcaccgg catgggtatt gaattcaaac tcaataccga agtgggccgc
    67681 gacgtgcagc tggacgatct gctgagtgat tacgatgccg tcttccttgg cgtcgggact
    67741 tatcagtcaa tgcgcggcgg gctggaaaac aaagacgccg atggcgtgta cgcagcgctg
    67801 ccgttcctta tcgccaacac caaacagtta atgggctttg gtgaaacccg cgacgaaccg
    67861 ttcgtcagca tggaaggcaa acgcgtggtg gtccttggcg gtggcgacac tgcgatggac
    67921 tgcgtgcgta cgtccgtgcg ccagggagcg aagcacgtta cctgtgccta tcgtcgtgat
    67981 gaagagaaca tgccgggttc ccgccgcgaa gtgaaaaacg cgcgggaaga aggcgtagag
    68041 ttcaaattca acgtccagcc actggggatt gaagtgaacg gtaacggcaa agtcagcggc
    68101 gtaaaaatgg tacgtaccga aatgggcgaa ccggatgcca aaggccgtcg ccgcgcggag
    68161 atcgttgcag gttccgaaca tatcgttccg gcagatgcag tgatcatggc gtttggtttc
    68221 cgtccacaca acatggaatg gctggcaaaa cacagcgtcg agctggattc acaaggccgc
    68281 atcatcgccc cggaaggcag cgacaacgcc ttccagacca gcaacccgaa aatctttgct
    68341 ggcggcgata tcgtccgtgg ttccgatctg gtggtaaccg ctattgccga aggtcgtaag
    68401 gcggcagacg gtattatgaa ctggctggaa gtttaagctc tgtcccagcg ccgcctcctc
    68461 agaaaagagt cggcgctgaa attctccttt ctttctggaa agcgcctcgc ataatctgaa
    68521 gaaaaccaat gtaatacgct catctggatt gttaccggta gatgcccggc gtatagctga
    68581 ttccattatt caacgcagcg ccatataaag ggacagataa tggaatcgct ctcagagaga
    68641 acctcaacag gctaccagca aatccacgac ggtattattc acctggtcga tagcgcccgg
    68701 acggaaacgg tacgtagcgt taacgcgctg atgaccgcga catactggga aattggccga
    68761 cgaattgtcg aatttgaaca aggtggcgag gcaagggctg cgtatggtgc gcagctaatc
    68821 aagcgactat caaaggattt aagtctaagg tataagcgtg ggttctctgc aaaaaactta
    68881 cgccaaatga ggctttttta cctctttttt caacatgttg aaattcgcca gacagtgtct
    68941 ggcgaattaa caccattggg aattccgcag acaccatctg cagaattccc atcggcaaag
    69001 atttggcaga cgctgtctgc caaattgaga ggctgtttga tatttccaca gcttttgtat
    69061 tgcatctcct attttcggtt tgtatattca tgcaaataaa cgaaggaaag ttgccatgat
    69121 ttacattgaa cagcttgaat taatccataa aagtggtgat gtgttgtatc cagtaaaaat
    69181 aaccagaaaa tcatcaggaa aaactgcctt ccatcttgtt ccattcggct taaataagac
    69241 tcacgacctt ctagaggtgg aagatgcttc agaagcaatt cggctggtga ttgatgaacg
    69301 tcattccatt cgctgcagta cacttacagc aactattacg aataaaaagg gcaagcgaat
    69361 taagcggaca ggtatataca gtatcaaggg tgttaacata aaagaataca acgtcaggta
    69421 atggcacaga gcggtccgtc agttcaggtt tggctctgtg ccaaagatgg gtcaggtcac
    69481 acctgatcta ataaatttaa gccattaaat cagccttaac cttcaccacc acctttttaa
    69541 tctcacccgg ctcgccgaca acgcatcctg gtttatgcgg ttcacttggc ataaacacgg
    69601 cgaacattcc cggttttaag atgatggctt gctcgttatc aatggtgctg caaagctgat
    69661 aatcatcctc atggtggaac tcttcacact ggcgcgcagt gcctgccatg ccaaacagaa
    69721 tccgttcctc accgtttaat aacagctgga tatcaatgta ttgctcgtgc aattccgctt
    69781 ttttctcgac gggcgattga gtgttaaacg tcatgacatt cataaagata ttgtcgccct
    69841 gtaattcgta acgaccaggc gctttttctt gcggtctggc agctaatgcc agcgttaacg
    69901 cgtcctgtaa cgcaggatgt aacccagcag acggtaatga ctgtacttca cccatcatca
    69961 taatttttct ccctgggcca acagcgcagc cccaagtaaa cctgcatcgt ggcggtagtg
    70021 cgccgccagt aaatcaacat gaaatgccgc tggctcctgc gccagatacg tttccaccaa
    70081 cgccagatac ccttctgcca gaccaacgct gccaccgacc accacgcact ggcaatcagt
    70141 tgtggcttta atatcagcga tcagccttgc cagcacatgt gcggagcggt gaatcagttg
    70201 ctgcgcctgc tcgtcgccct gcccggcgcg tgtgaaaata gttctcgcat ccgcgcccat
    70261 gagttcacct tgcgctgccg ctgcaatacc gcgaccagaa gcaatcgctt ccacgcaacc
    70321 tgtgcgtcca cagccgcaga ctgggccgtg tggatcggca agcgtatgcc cgatgtgccc
    70381 cgccagaccg ccagggccgg taagcagttt accgccgctc actacaccgc cgccgacgcc
    70441 agtggaaacg gtgataaaca ccatatcggt tatatcgcct tccagcgcct gatactctgc
    70501 ccatgctgcg gcctgcgcgt cgttaatggc aatggtcggc aaattggaaa gttgttccag
    70561 cgttttgact aacggaaagt gtagcaatcc accaagatta tgcggattca gcgccagcaa
    70621 gctgccgtcg cgaataattc cggttgaagc gatggcaacc tgctgcgcat gagcttgcaa
    70681 cggggcgact aacgcggcta aggcatcacg caacgcctgc ggagtctggc tggctggtgt
    70741 cggaagttct cggcgatcgc ggatctgccc gtcagcgcca atcagcgcgg cggcaagttt
    70801 agtaccgccg atatcaatcg ccaacgtggt catagcaccg cctttttcat cgctgtgttg
    70861 taccactgac aaatgtgttc aagacgcgta atggcagaac cgaccgtcac cgcccacgcg
    70921 ccgtggcgca tcgcatctgc cgcctgagca ggcgtgttgt aacgcccttc ggcaatcacc
    70981 cgacatccgg cgtcgctcaa cgttttcacc agcgccagat ccggctcttc tggcgtttca
    71041 ggcgtggtat agccagaaag cgtagtgcca ataatttcgg ctcccagctt ttggcatgcc
    71101 aggccgtctt ccggcgttga gcagtcggtc atcgccagta aaccgtgatg gtgaatacgt
    71161 gccagcagcg tttcaacagg caccggacgc gggcggtcgg tgccgtcaat ggcgataatg
    71221 tccgcgcccg cctgcgccag cgcatcaaca tcttcaatat aggccgtgat gcgtaccgga
    71281 gaatcctcca gatcgcgttt cacaattcca ataatcggca cgctcaccac cgcacgcgtg
    71341 gcttgcagat ttgccacacc ttcaatgcga atggcaaccg cgcccgcctg ttctgccgct
    71401 aatgccatgg cagcgacgat ttcgggttta tcgagcgggc tgtccggaac cggctggcag
    71461 gagacaatca ggccaccgtt agcagcgatt ttttgatcca gttgtgcaag taacgacata
    71521 catcttccct tagcgaaagg cccggtacag agaccgggca acaggattaa cttttggttt
    71581 tgactaaatc gtttttggcg ctgccaaatg gcacggcacc gctaaatggt ttgccgtcga
    71641 tagcgtcatg agtacgcaat gcttccgggc gcagccaacg ctgaacgcgg gaaggcatat
    71701 ccagaccaat cagcaggatc accacaaatg tcagactgaa cgatagcgat gccagcgcag
    71761 tacccagatc cagacgttga gcgatcaacg cgccgatgat tggggccagt gcgccgccca
    71821 atgcgccaac gttgtaggta aagcccaggc ccgctgcacg ctggtcggta tcgaaataac
    71881 cgccaatcag tttcggtaag atcccggcga tcccttgtcc aagcatttgc tggaagaaca
    71941 gtaacagacc gagcacccag acgtttgcgc cgccaatcgc aaataccgga ataatcaaca
    72001 gctgcgaggc caacaggcta caaacgtagg ctttgcgggt tcccagccag tcaccgagga
    72061 agccgctgac acagcacccc accgcagccc caaagccgct aaagaacagc acattggcga
    72121 cagtatgcgg gttataagcc agatcggttt tcagatacgt tggcagcagc gcctgaatcg
    72181 gccatgagta gaggaaagca aacaacacga ccaccatcag cattacgccc gttggccagc
    72241 gtttacccgt actttgcacc ataaagctga taaagattgc ggcgcataac agcccaagaa
    72301 cagcgacgat cgcggcattt tgcaggttac cggcgaagca aaaccacagc gcagtagccg
    72361 ccgccagtgt cattacgata ttggcaatgc gatgttcacc acggtagaga atatctacca
    72421 tcgtgcgtac tggtgcttta cctgcatgct tctctttcca gtcttccgct tccgggatgt
    72481 ttttacgcag ccagagagca aagatgatag gcaaaatgcc gataaagaac agcgcacgcc
    72541 agccccagac cggaaccacc aggctataga cctgagcggc aacgacggcc cccacagaga
    72601 agcctgaaat cagaaaacca ctggctttgt tacgcagatg ttttggccag ctttcaatga
    72661 cataggtggc gctggaaccg tattcacccg ccatccccat gccgatgacc agacgcgcga
    72721 taaacatggt gatgtagcct ggcgcaaagc cgcaggccag cgtcccggcc gagaagagaa
    72781 cgatgctggt gaccattgcc agacgacgcc cgtagcggtc acccatagcg ccgagcatca
    72841 ggccgccgaa ccagcgagag ataaaggctg cggagatcag acttgccgcc tgcaccgtcg
    72901 tcagcccgaa ttcaccctgt acttcggtga gtaccagggc gattaaaacg aaatcaaaac
    72961 cgtcaagcag atatcccaac caggcagcgg aaaatgcgcg ccattgtgca cggttgagat
    73021 ggcgatacca cgggatattc tgggttgtag tactcattgt gagtctcccg cggtgggcga
    73081 tgcccacacg ctttggtata aaaattgtag ggtacagatg cgtttatttc ccctcacccg
    73141 gtaggggcga gcgaggggaa acaactcacc cgcgctcttg catcaactgc tgggccagcg
    73201 ccttcagttc tggcagatat ttttcatcta ccggtccaaa cggtttgcgg cacagcggca
    73261 cagaaacgac atccatataa tggaggacag ttttcaggcc gcggaatacg cccgttttga
    73321 tcagtaaatc aatgacttta ttgcattcag tttgcagttt ctgcgcggtc tggatatcgc
    73381 cttctttcag cgccttaacg atcccctgat agcgccagcc catgatgttg taggtactac
    73441 cgataccacc atcagcgccc gccagcagac cagaggcgaa gatttcgtcg taaccgttat
    73501 agagcacaag atcaggatgt tcacgacgga tctgctccat ctgatagaga tcgccagagg
    73561 tctgtttcag cgcacctacg ccaggcaatg taacaagtgt gttgatctga tccagggtca
    73621 gttttacccc actcagggct ggaatgttgt acaccaccat cggcaaacca tccgccgaat
    73681 caataattgc ccgatagtga tcgcagtgtt cttcaaagct gaaaggatag tagaacggcg
    73741 tgacggcgga gacggcatcg aagccataac gtttagccga tgccgcaagt tgttggcttt
    73801 cggcggtgct gacgcaaccg acgtgggcga tgagtttaat cttacctttc gcctcttcgg
    73861 cgacgatttc cagtacctgt tcacgctcgg aaaggctttg tacaaaggcc tcgccggtcg
    73921 aaccacccac gtataaaccg tcgatgccct gctgaatatt gaactgaacc aggcgacgca
    73981 gactcgcttt atccagtgct tgttgttggt caaaaggagt caggagtgca gccattacgc
    74041 cacgtaaatt cgttgccata aatacctctg aagtgatgct tgtctgataa acgatatacc
    74101 tttatacctg ttataccaga tcgattaagc aacaccccat acagaaagct tataatgcga
    74161 tctgcttcac taaagtggca ttatttcttt ttgttggtgg tctgaccgaa agcgtgccag
    74221 gtagcagaga cgctgttgag atgcgattgc aacgcacgat cggcttcgtc aggatcatga
    74281 cggcggatcg catcaacgat cgcaatatgc tgttgataac taacgttgtt atgttcgtgc
    74341 agtgcctgat cggtaaccgt tgggcgtgcg gcaataagcc agtcgagcag ggcaacgtgg
    74401 atcgccatga agattgggtt accggggatc tccgccagca cgcggtggaa atcaacgtct
    74461 gaacgaatga atgccgcgtt gttatccagc gactgactgt tgatttccag tgcttttgcc
    74521 agcaaatcga tttgctcatc ggtggcatgt tcagccgcat agcgcaccag actggattca
    74581 aagaacagac gtaattgttc gaaatgggca atcccaccgg gatgagaaag gaaatctttc
    74641 gccatgccgg aaagctcacc gatgatagtg tccgcagaag gacgcgagac gcgagcgcgt
    74701 tcgccgttgt ttatttgcac cagacctttg cgttttaacg ctgccagcgc ttcacgcacc
    74761 gaaggacgcc cgacgttaaa gaacgccatc agttcgcgtt cagacggtaa ttgttcacct
    74821 tcgccaaatt cacgacggcg gatcatctgt tccagctctt cttccaccat ttcggagagt
    74881 tttttacgcg ccagcgggcg gctacgcaag ttgcgaccaa ttgcaggtga agaatcttcg
    74941 gtttgcgaat caaatgcgtt cataaggccc attctgtaag gtcagtgtga ttaacatcat
    75001 cagtgacatc ctatcacagg attgagagta ggggaaaatg gcaggttttt ctctttgtgc
    75061 ctcatcatta ccataattaa cggaataatt aactattgcg aaaaattaat gtaacgcaga
    75121 taaaaacatc ccgtttgaat tatttataag actattcacg agcattatga atattatgaa
    75181 tgcgttcttg caaaataatc ataagcgcat attttttaat gaaaaattat ctcacctaca
    75241 attaaaaaca cgacatccgc accataaata gacttgcaaa aaatataaca tcattgtttt
    75301 caatctgccg tttatgggat tgaccgtttt cttttgacac ggagttcaac aatgttcggc
    75361 ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa aaactacaaa
    75421 cctcaggtgg tgctggctgc cgcaggtatc ttcctgatga tgtgcggtgt ctggttaggg
    75481 ttcggtggtg tactcgagcc cgccaaaagc agcggctact tgatcgtcga tatttataat
    75541 gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctatcgat tatggcggtg
    75601 ggcggtatgc ccgctacatg gagcgcatag gggccagtcg cgcgatggtg agcttgttaa
    75661 gccgcccgtt aaaactcatt cgctcaccgt atattattct gtcggcaact tacgtcatcg
    75721 gtcaaatcat ggcgcagttt attaccagcg cctccggtct gggtatgttg ctgatggtca
    75781 ccttatttcc gacactggtg agtctgggag taagtcgtct ctctgcggtg gcggttatcg
    75841 caaccacaat gtccattgag tggggaattc tggaaacgaa ctccattttt gcagcccagg
    75901 tcgcgggaat gaaaattgcc acttacttct tccactacca gcttccggtc gcctcttgcg
    75961 tcattatctc ggtggcgatc tcccactttt tcgtgcaacg cgcttttgac aaaaaagata
    76021 aaaatatcaa tcacgaacaa gcagagcaaa aagctctcga taatgtcccg ccgctctatt
    76081 acgccatttt acctgtgatg ccgttaatcc tgatgctcgg ctcgctgttc ctcgcccacg
    76141 tcgggctgat gcagtcagaa ctgcatctgg tggtggtgat gttactgagt ttgacggtga
    76201 cgatgtttgt tgagttcttc cgcaagcata acttgcgcga aacaatggac gatgtgcagg
    76261 cgttttttga cggcatgggt acgcagtttg ccaacgtggt aacgctggtg gtcgcgggtg
    76321 aaatatttgc gaaaggctta acgacgattg gcactgtcga tgcggttatc aggggtgcgg
    76381 agcattctgg tctgggcggt attggcgtga tgattattat ggcgctggtc attgccattt
    76441 gtgccattgt gatgggctct ggcaatgcgc cgtttatgtc atttgccagt cttattccga
    76501 atatcgcagc cggactacat gtaccagcgg ttgtaatgat tatgccgatg cattttgcca
    76561 cgacgctagc gcgcgcggtt tcgccgatta ctgcggtggt ggtcgttacg tcaggaattg
    76621 caggcgtttc gccttttgcg gtggtgaagc gaacagcgat ccccatggca gtcggtttcg
    76681 tggtgaatat gattgccaca atcacgctat tttattaagc cattaaaaga caaaacaggc
    76741 caccagggcc tgtttttgta ttacttcaca acgcgtaatg ccggtcgacc accgcgtggt
    76801 ggctgcggag gttcatcgtc aggatgagtg tcatcatcgt gatctggctt gtcgccatca
    76861 ataaccgaca taacggtttc gttgtctgcc gatgcctctt catcattcat gatgctggta
    76921 tcttcatcgt aggcagcttc aggctcaaac attgtgcctg cgccattttc acgggcgtag
    76981 atagccagca cggcagccag cggcacagaa acctgacgcg gaatgccgcc aaagcgcgcg
    77041 ttaaagcgca cctcatcatt cgccagttcc agattgccga cagcacgcgg cgcaatgttg
    77101 agtacgattt gcccgtcacg cgcatattcc ataggaacct gcacgccagg gagcgtcaca
    77161 tccaccacta ggtgcggcgt gagctggtta tccagcaacc actcatagaa tgcacgcagc
    77221 agatagggac gacgtggtgt tagctgtgac aaatccatac agattaactc cggcccagac
    77281 gcatttcacg ttctgcttca gttaaagaag caaggaaaga gtcacgctca aagacgcggg
    77341 tcatatagcc tttcagctct ttcgcacccg ggccgctgaa ctcgatgccc agttgcggca
    77401 gacgccacag cagcggagca agatagcaat cgaccaggct gaactcatcg ctcaggaagt
    77461 acggcttctg accgaagacc ggcgcaatcg ccagcagttc ttcgcgcagt tgcttacgtg
    77521 cggcatctgc ttcagaagct gaaccgttga tgatggtgtt catcagcgtg taccagtctt
    77581 tttcgatgcg atgcatgtac agacggcttt caccgcgagc taccgggtaa acaggcatca
    77641 gtggcggatg cgggaaacgc tcatccagat attccataat gatgcgagat tcccacaggg
    77701 tcagctcacg atccaccagg gtcggaacgc tctgattcgg gttgaggtca atcagatcct
    77761 gaggcggatt gtccttttcc acgtgttcga tctcgaaact tacacctttc tcagccagca
    77821 caatgcggac ctgatggcta tagatgtcag taggaccgga aaacagcgtc attaccgaac
    77881 gtttgttggc agcgacagcc atgaaaacct ccaggtatag tcagaatttt tactgctacc
    77941 agccaccagg tggccagtca gaagttgtgt tacccagtaa ggaacgactc tctttgttcg
    78001 aaaatcaaac aaaaaatgag caatacccga catttgggca gaaaattgga tgatagttta
    78061 ccagattttg cgacctttgt ggtgagtcga tgccggaaat ggggaaaaag agatgcgctt
    78121 tagtctgaaa tagttgactt agtcccttat tggcgatgtg gtttttgttt tacctgtctg
    78181 tcaggtggca gcaaaaagca actttccagt ttttacgctg attcagattt tagctataaa
    78241 aaaacccgcc gaagcgggtt ttttcgaaaa ttgttttctg ccggagcaga agccaattaa
    78301 cgtttggaga actgcggacg acgacgtgct ttacgcagac cgactttctt acgttcaacc
    78361 tgacgagcgt cacgagtaac gaagccagct ttacgcagtt cagaacgcag ggactcgtcg
    78421 tattccatca gagcgcgggt gataccgtga cggatcgcac cagcctgacc agagatacca
    78481 ccacctttaa cggtgatgta caggtccagt ttctcaacca tgtcgaccag ttccagcggc
    78541 tgacgaacta ccatgcgggc agtttcacga ccgaagtact gttccagaga acgttggttg
    78601 attacgattt taccgttgcc cggtttgatg aaaacgcgag ctgcggaact tttgcggcga
    78661 ccagtgccgt agtattgatt ttcagccatt gcctataatc ccgattagat gtcaagaact
    78721 tgcggttgct gtgccgcgtg gttgtgctcg ttacccgcgt aaactttcag tttacggaac
    78781 atagcacgac ccagcgggcc ttttggcaac atgcctttaa ccgcgatttc aatcacacgc
    78841 tcaggacggc gagcaatcat ctcttcaaag gtcgcttgtt tgataccacc gatgtggccg
    78901 gtgtggtgat agtacacttt gtcagtacac ttgttgccgg ttacagcaac tttgtcagcg
    78961 ttcagaacga tgatgtaatc accggtatct acgtgcggag tgtattccgc tttgtgctta
    79021 ccgcgcaggc gacgagccag ttcagtagcc agacggccca gagttttacc ggtcgcgtca
    79081 acaacatacc agtcgcgttt tacggtttct ggtttagctg taaaagtttt cattaaaagc
    79141 ttacccaata aatagttaca cgttggtgaa cacccaaacg tcttcaattg ttgaggttca
    79201 cacgacaaag tccggcaaac ctaccccttc gaatagccta tgccagcaca caaaaagttt
    79261 tgggaaaaaa ctttcttgta acgtggggtc gcaggattat agagaagtcg gggtcaaaga
    79321 tcgacccctt tttgtgattt gtgacaggtt ttaacccgcc aaatgctcgc gcttcagata
    79381 ctcttcgctt tgcatctctt gcagacgtga caggcaacgc tggaactcaa acttcagccg
    79441 atcgccctga taaatttcat acagcggcac ttctgcactc accactaatt tgacatggcg
    79501 ctcgtaaaac tcatccacca gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt
    79561 cataactggt acatcaaaca acatgaccgt atgaaagagg cgtgagagcg caatatagtc
    79621 atgctggctg cgggcatcga cacacaaggt ggtaaaagag acagcaagcg tctgattctc
    79681 agcgcccatt gtcgccagcg gtcgatggtt gatttctaac gtcggtgaat tttcgcgttt
    79741 cgctcccgcc agcgccaacc atagtttatc catttgtgcc cgggtttcat cgtggagtgg
    79801 cgaaagccac agatgcgcct gagtgagtgt acgcagtcga taatcaacac cagcgtccac
    79861 gttcattaca tcacaatgct gtttaatggc atcgattgca ggcagaaaac gcgcacgttg
    79921 caagccattt cgataaagtt cgtccggcgg aatatttgac gttgctacca gggtaatacc
    79981 gcgggcgaac agggctttca tcagaccgcc aagtagcatg gcgtcggtaa tatcagaaac
    80041 aaaaaattcg tcaaaacaga gcacgtcagt ttcggcttta aagcgatcgg caataatttc
    80101 cagcggatcg gtctgcccct gtaaggcagt tagctcttcg tgcacccgca gcataaaacg
    80161 gtgaaagtgc aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa ggtccatcag
    80221 ccaggttttc ccgcgtccta caccgcccca catatataag ccacgcactg gcgtatgctt
    80281 tgtgtcttcg cgtttacccc acagcttacc gacccgcgcc attagcccac tcgtcctggg
    80341 ggctggtggc ctgctattga tgagttcttg ataaataatt tccaggcggc tgacggcctc
    80401 tttttgaacg tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt attgcgatgt
    80461 tggggtaacg ctttgcatga tcttattatt attccttgaa taatcggtgc gccgttgttc
    80521 acggttgacg aaaaaaaggc cgttctacac tacgcgatat gcagtcggga ttccacttct
    80581 gtggaattaa cggttatagt ggcataatca gccgtaggca tggagcctga agccaacacc
    80641 ctacggaaac aaaagacaac gggagatgtt catgacctgg gaatatgcgc taattgggtt
    80701 agtcgtcggc atcattattg gtgctgtggc catgcgtttt ggtaatcgta aactacgcca
    80761 gcaacaggcg ttgcagtacg aactggaaaa gaataaagct gaactggacg agtatcgcga
    80821 agagctggtt agccactttg cccgcagcgc ggaattactg gataccatgg cgcacgacta
    80881 tcgccagctg tatcagcaca tggcgaaaag ctccagcagc ctgctgccgg aactgtctgc
    80941 tgaagcaaac ccgttccgta atcgtctggc agagtctgaa gccagcaacg atcaggcacc
    81001 ggtgcagatg cctcgcgact attctgaagg tgcatccggc ctgctgcgta ctggcgcgaa
    81061 gcgcgactaa tttatttttc gggcgcagcc attgcgccct cctcttctct ccctccccga
    81121 ctatcattta atctggtgtc tcattgttag ccgtctgaaa attcaataac atcaaactgt
    81181 tttgaatctc ttttcttatc attcaggtac gagagcagga ataatgaaaa aacaaaccca
    81241 gctgttgagt gcattagcgt taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt
    81301 cgcgtcgatt ccaggccagg ttgccgatca ggcccctctc cccagtctgg ctccaatgct
    81361 ggaaaaagtg cttccggcag tggtgagcgt acgggtggaa ggaacggcca gtcagggaca
    81421 gaaaatcccg gaagaattca aaaagttttt tggtgatgat ttaccggatc aacctgcaca
    81481 acccttcgaa ggtttaggtt ccggtgtcat catcaacgcc agtaaaggct atgtgctgac
    81541 caacaaccat gtgattaatc aggcacagaa aatcagtatt cagctcaatg atgggcgcga
    81601 gtttgatgca aaactgattg gtagcgatga ccagagcgat atcgccctgt tacaaattca
    81661 aaacccgagc aaattaacgc aaatcgctat tgccgactcc gataaattgc gcgtcggtga
    81721 ttttgccgta gcggtcggta acccatttgg ccttgggcaa accgccacct ctggcattgt
    81781 ttccgcatta ggccgcagcg ggttgaatct tgaaggtctg gaaaacttta tccagacaga
    81841 tgcttccatt aaccgcggta actccggcgg tgcactatta aaccttaacg gtgagttaat
    81901 tggcatcaac actgcaatcc ttgcgcctgg cggcgggagc gtcgggattg gatttgccat
    81961 ccccagtaat atggcgcgaa cactggcgca gcagcttatc gactttggtg aaatcaaacg
    82021 cggtttgtta ggcatcaaag gcaccgagat gagtgccgat atcgccaaag ccttcaacct
    82081 tgacgtgcag cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct cggcaaaagc
    82141 gggcgtcaaa gcgggcgata ttattaccag cctcaacggc aaaccgctga atagctttgc
    82201 tgagttgcgc tctcgtatcg cgactacaga gccgggcacg aaagtgaagc ttggcctgct
    82261 gcgtaacggc aaaccactgg aagtagaagt gacgcttgat accagcacct cttcgtcggc
    82321 cagcgctgaa atgatcacgc cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa
    82381 agattgcggc aaaggtatta agatcgatga agttgtcaaa ggaagcccag ctgctcaggc
    82441 aggcttgcaa aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga actcgattgc
    82501 tgaaatgcgt aaagtgctgg cggcaaaacc ggccatcatc gccctgcaaa ttgtacgcgg
    82561 caatgaaagc atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca ggcttacgtg
    82621 tgatgtccgg ttaactcgtg gtatgctgct gccgttccct tttttaatga cgcctccatc
    82681 atgtttgtga agctcttacg ttccgttgcg attggattaa ttgtcggcgc tattctgctg
    82741 gttgccatgc cttcgctgcg cagccttaac ccgctttcca ctccgcaatt tgacagtacc
    82801 gatgagacgc ctgccagcta taatctggcg gttcgccgcg ccgcgccagc ggtggttaac
    82861 gtttacaacc gtggtgtgaa caccaactct cacaaccagc ttgagatccg caccctggga
    82921 tccggtgtaa tcatggatca acgcggttat atcatcacca ataaacacgt catcaacgac
    82981 gccgatcaga tcatcgtcgc cttacaggat ggacgtgtat ttgaagcatt gctggtggga
    83041 tctgactctc taaccgatct ggcggtactt aaaattaatg ccactggcgg tttacctacc
    83101 attccaatta atgcacgtcg cgtaccgcac attggcgacg tagtactggc gatcggtaac
    83161 ccgtacaacc tcgggcagac cattacccaa gggattatta gtgccacggg gcgaatcggt
    83221 ctgaacccta ccgggcggca aaacttccta caaaccgatg cttccattaa ccacggtaac
    83281 tctggcggcg cgctggtgaa ctcgctggga gaactgatgg gcattaacac gctgtcgttt
    83341 gataagagta acgatggcga aacgccggaa ggtatcggct ttgcgattcc tttccagtta
    83401 gcaaccaaaa ttatggataa gctgatccgc gatgggcgcg tgatccgcgg ctacattggc
    83461 attggtggac gtgagatcgc accactgcac gcgcagagcg gtggtataga tcaactgcaa
    83521 gggatcgtgg ttaatgaagt gtcacctgac ggcccggcgg cgaatgcggg tattcaggtc
    83581 aacgatctga ttatttcggt ggataacaaa ccggccatct ctgctctgga gacgatggat
    83641 caggtggcag aaattcgccc tggttcggtg atcccggttg tagtgatgcg tgatgataag
    83701 cagttaacgc tgcaggtcac cattcaggaa tatccggcaa ccaattaagt cgagtgctca
    83761 aaacaaaaaa ccggagtctg tgctccggtt ttttattatt cgctaatcaa ttacttatta
    83821 acgaactctt cgcccagggc gatatctttc ttgagcgtat ccagcatacc ttccagcgcg
    83881 ttctgttcaa atgcgctcag ggtaccgata gatttacgct cttccacgcc gtttttaccc
    83941 agcagcagcg gttgagagaa gaaacgggcg tactgaccgt cgccttcaac gtaggcacat
    84001 tcgacaacgc cttgttcgcc ctgcagtgca cgaaccagag acagaccaaa acgtgcagct
    84061 gcctggccca tagacagggt tgcagacccg ccaccggctt tcgcttcaac cacctcagta
    84121 cccgcgttct ggatacgttt ggtcagatca gccacttcct gctcggtaaa actaacgcca
    84181 ggaacctgtg acagcagcgg cagaatggta acaccagagt gaccgccaat aaccggcact
    84241 tcaacttcgc ctggctgttt gcctttcagt tccgcaacaa aggtgttgga acgaatgata
    84301 tccagcgtgg taacgccgaa cagtttgttt ttgtcataaa caccggcttt tttcagcact
    84361 tcagcagcaa ttgcaactgt ggtgttaacc gggttagtga taataccaat gcacgctttc
    84421 gggcaggttt tcgcaacttg ctgtaccagg tttttcacga tgccggcgtt aacgttaaac
    84481 aggtcggaac gatccatacc cggtttacgc gctacacctg cagagataag aacgacatct
    84541 gcgccttcca gcgccggagt cgcatcttca ccagaaaaac ctttgatttt cacagcagta
    84601 gggatatggc tcagatcgac agccacaccg ggagtcactg gagcgatatc atacagagag
    84661 agttctgaac ctgaaggcag ttgggttttt aacagtagtg caagcgcctg gccaataccg
    84721 ccagcagcgc cgaggactgc gactttcatc ctaaactcct tattatattg ataaactaag
    84781 atatgttgct ccgctgccgc gaccttaatc cacaaaattg ccgtttacaa tgaccacata
    84841 tcaagaatgt gtagtcacgc aagtttagcg tttatgcatt taattgccgt aatcaggaac
    84901 ctaacttacg taattaacag tcgcttatgc attagcgcaa cattccaaca ggtggtgaca
    84961 atatacccta ccgttcagcc aaaacaacat caatttgata acaattaatt tacttttaag
    85021 cagaatttgc atgccgtgac gcaggcatgt ttctcaataa cgaaatttga taaaatcccg
    85081 ctctttcata acattatttc agccttcttc agggttgact gtttgcataa aaattcatct
    85141 gtatgcacaa taatgttgta tcaaccacca tatcgggtga cttatgcgaa gctcggctaa
    85201 gcaagaagaa ctagttaaag catttaaagc attacttaaa gaagagaaat ttagctccca
    85261 gggcgaaatc gtcgccgcgt tgcaggagca aggctttgac aatattaatc agtctaaagt
    85321 ctcgcggatg ttgaccaagt ttggtgctgt acgtacacgc aatgccaaaa tggaaatggt
    85381 ttactgcctg ccagctgaac tgggtgtacc aaccacctcc agtccattga agaatctggt
    85441 gctggatatc gactacaacg atgcagttgt cgtgattcat accagccctg gcgcggcgca
    85501 gttaattgct cgcctgctgg actcactggg caaagcagaa ggtattctgg gcaccatcgc
    85561 tggcgatgac accatcttta ccacccctgc taacggtttc accgtcaaag acctgtacga
    85621 agcgatttta gagctgttcg accaggagct ttaatctctg ccccgtcgct tctggcggcg
    85681 gggaaaatgt tgcttatccc tctcaacccc ctgctttccc ctgcgattaa tttaacgaat
    85741 agtgcgtttt actgcgacat gtcattcaca taatgaatac atacggtaaa aaaagcacat
    85801 tatgcaaaat tcattatcta attgaaaata ctagaattaa aaataaatga ccgtgttttt
    85861 aattcttttt tgttattaaa attcacattt ttaacactta gtatcaactg aaacagttag
    85921 cgtggtatta attagttcaa taattagtgt atgcttgatt ttgtgatatg ggtcacaaaa
    85981 caaaggccca gctaaaagat tatgtcgagg taaaaatcat gaaaatcaaa accactgtag
    86041 ctgcattaag cgtactttct gttctctctt tcggtgcatt cgctgccgac tccattgatg
    86101 ctgcacaagc acaaaatcgt gaagcaatcg ggaccgtatc cgtaagtggt gtggcgtctt
    86161 cgccaatgga tatgcgtgaa atgctgaaca aaaaagcgga agagaaaggc gcaacggcct
    86221 accagattac tgaagctcgt agcggtgaca cctggcacgc tacggctgaa ctgtacaaat
    86281 aaaccctcat cgtcttgtcc gacgatattg cccccggttc gggggctttt tttgcgctaa
    86341 tgacgaacat taaaacgcaa atgcccttcc agctcttcct ctgcctcatc aaacagcaat
    86401 attaacgcgc caaaacggcg acgcgttttc tctcccagat gaacaaattc aatctcaagt
    86461 ggtagcggca ggacatcgtt cattaacaca tcccatagtg agtcgagatc gcgtacctta
    86521 tctttcgcca gaccaaaggt ttggctaaag tcacgataaa aatcctcctg actctcaatc
    86581 tcatcaaaat caaaggtata aatattcatc tattgccacc gtcacgtttc gcggcgagtt
    86641 ctgccgtgca tcgctaagta tagccatgaa aaaaccgaca cttttggcgt cggttttgac
    86701 ctaactatcg gtcaacgcat gttgatactt atggagcata cccgccagcc gatgaaccgg
    86761 ttccgtcacc tgcggtggcg cttgccagat gcgtagcttt tcctggtaga tttcaagttc
    86821 ttccagcaac tggccaaagt accgacgacg tttatcatcg ctacgggcag atatcacatg
    86881 gtctgctgtg cggcgcattt gtcggtgaaa cgccgataaa tcctcgttaa ccgggatcgg
    86941 cgcatcacgc aggcgctggt gcgcgataat catcgtcagc gccaggcgaa atttcggcaa
    87001 atcccctggg aacttattca tcagcaaaaa cagctgctga taaagtgccg ggaggtggtt
    87061 ctctttacga cgtgccacat tggtagtcat cgcggaaaca gcggcagaaa caaactgatt
    87121 aagcagtaca cgttcggtcc tgtcgcgcga tttatcccgc acaagcaaaa taacggtgaa
    87181 cgcgagcaca cagccgacga tttgccctaa tgcgctgtcg agaaactgac tgaaatggaa
    87241 agtcatcggg ttatccagca cgataatatt tatggtgctg gccagcgccc ccatcgagcc
    87301 cagtcgccgt tgctgtactt ctataccgag gaaaaatccc agcactgcca ggctaataca
    87361 cagcagcaac atgctctgtt gggtattagg gataatcacc aaaaagtaga gcagccctaa
    87421 cggcagcgcg gccagcgtcc cgtagataaa gtcgatcgcc accatgcgtg gattcggcaa
    87481 acgcattgcc agtgacgtca ctaccgcaat catcaccatt gcaccactgc cggaagtcca
    87541 gcccgtccac agccagaaaa gcgtgcccag aatgcaggaa agtgtggttc gccagaagtt
    87601 aaccatcgca tgatgacgtt cggctgactc tactttgact tccggttcgc cttgcaggat
    87661 ctcttcttcg gtggcgttga tttttgtgtt actgataacg ccgcgcttga gaagctgata
    87721 acgcgttgcc gccgcgaccc agctataaat ggtgacaggc gtttcccgtt ccccggtcca
    87781 ggcgataact ctccgcaggc gtttgagctg cttgtggacg tcctgcgcgg tttctaccgg
    87841 cgtgtcaaaa aattcgcgga aagtatcagt gatcaattcc gggcgcgtat tctgaataag
    87901 ataagtttcg caggattggg taatcagcgt cagcgatagc gtattgatcg cttttaaacg
    87961 tcgattggcc cgcgcccagc gggaagattc catattcagg ttgctgcgca tgccttgtag
    88021 cgccgtggtg cgtcgcacca ggtcgcccca ggctttatcg acaacttcac catcgccatg
    88081 cttgatacag agttgcatta attgatattg cgcgaccagc aaactttcca gctctcgatc
    88141 cacttcttgt ttgatcgatc gcggagaaaa gagcaaatcc gctataatcg cacacacaat
    88201 accgataacg atctcgctac aacgttcgac ggcaaactgc ggcgtaagca atggttccgg
    88261 ctgaatggtg atcacaatga tcagcgcggt ataaccggcc agcccccacg cgtacgagtt
    88321 ttctattcgt accagcgagg atatccaggt acaaaaaccg gcccagatac agcacaccag
    88381 aatcatcaat aatggtgcgc ggatcatcgc aatgatgatc accagtccgg caatacagcc
    88441 aataaatgtg ccgatgatgc gcaaaaagcc acgataacga atagcgccag aatacggttc
    88501 acctcccgca gcaaaggccg gaccggcggc aacaatcgcc gctgtcagta ccgcccagcg
    88561 tggcgtttcc agctggaagt gaaagccaac aaacagcgcc agtacaatgg cggtcgccag
    88621 ttttaccgca aagcgaatat gttggttagc aatggagaaa atacccatcg tgattaacca
    88681 aactcacgca ggcgatgggc cattttacgg aagaacgaat cctggctttc gtcgcgatct
    88741 tgtttgccag tgaccaccac tgtagcagtg gtgcccgcag gccagatgtt ctcttgctgg
    88801 ttgtcgagac gaatacgaac cggaacacgt tgcgcaagac gcacccattc aaggttagag
    88861 tctatagtcg ccatcccttt gtcgtcacgc gtgctgctgg cgttggtgac ccctgcggca
    88921 acactatcaa cagtcccttt cagcactttg ttactgccaa gcggcgtgat ctctgcacga
    88981 taccccggac gcaccccttc cagcttagtt tcttccatat aggccagtac atagaaggag
    89041 ttctgtttca ccagcgcaac cgccgttgat cctcgagtaa taaactcacc ggtatagacg
    89101 ttgaggttgg tcacccagcc atctgctggc gcgcggatca ccgtgcgttc aagatccagt
    89161 tttgccagat cgcgggtcgc ctgcgctttc gctaactgat gcagaaccgt ttgtagtacg
    89221 ttgttagcct agtcgatctc ttcgcgagac atcgcctgca caccgagacg gttacgacgt
    89281 ccggcctcct ggcgtttctc ctgtgccagt acctgataat aagcaacatc ggcttgcgct
    89341 tcctcaagcg ccttttgata gcgcggctgg tcgatggtga acagtatctg tccttttttc
    89401 accagctggt tatcatgaac attcacctgg gtaatgagtc cagaaacgtc cggcgcgatc
    89461 gcaatgacgt cagcgctaaa gcgcgcgtca cgcgtccagg gggattcggt gtaatagacc
    89521 caggcattaa aaattgcgat gaaggccaga atgactaata cgaccgtgat ggccgtacgg
    89581 gagaattttc ttattagtgt tttcacttca acctcaaacg aacagtcgcg atatcaaata
    89641 aaacaagcag caatagagcg cggtgttgaa caacgccgga tgccagacaa agtcgtagat
    89701 acctgttggc acaagtaccc ggcgcaccag ccagaaaatc gccagtgata aaagcaattc
    89761 aaaaaatatc ggtgggaagg acagcccaaa caccacgata acgggaaaca gactcatgtt
    89821 gaccttggtt gtatagagag agcaggcgtt attattttca gcatctgtcg ccgcagagaa
    89881 gggcatggaa agccgggcga gagcaacatt gctgtagatt gatatttaat atattagcgt
    89941 aactgttatg ctgttatcta
//
LOCUS       DAJTOR010000002        80836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTOR010000002 DAJTOR010000000
VERSION     DAJTOR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 80836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.002"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            404..1606
                     /gene="proV"
                     /locus_tag="OO249_000095"
     CDS             404..1606
                     /gene="proV"
                     /locus_tag="OO249_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5634533.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1599..2663
                     /gene="proW"
                     /locus_tag="OO249_000096"
     CDS             1599..2663
                     /gene="proW"
                     /locus_tag="OO249_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5634534.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQVLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPSIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2721..3713
                     /gene="proX"
                     /locus_tag="OO249_000097"
     CDS             2721..3713
                     /gene="proX"
                     /locus_tag="OO249_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5634535.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTLLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKEPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAALK"
     gene            4005..5189
                     /locus_tag="OO249_000098"
     CDS             4005..5189
                     /locus_tag="OO249_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5634536.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRPRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            5313..6050
                     /gene="ygaZ"
                     /locus_tag="OO249_000099"
     CDS             5313..6050
                     /gene="ygaZ"
                     /locus_tag="OO249_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5634537.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            6040..6375
                     /gene="ygaH"
                     /locus_tag="OO249_000100"
     CDS             6040..6375
                     /gene="ygaH"
                     /locus_tag="OO249_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5634538.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6466..6996
                     /gene="emrR"
                     /locus_tag="OO249_000101"
     CDS             6466..6996
                     /gene="emrR"
                     /locus_tag="OO249_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5634539.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            7123..8295
                     /gene="emrA"
                     /locus_tag="OO249_000102"
     CDS             7123..8295
                     /gene="emrA"
                     /locus_tag="OO249_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR5634540.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTVREISLAPVNKLIDDIVKANAG"
     gene            8312..9850
                     /gene="emrB"
                     /locus_tag="OO249_000103"
     CDS             8312..9850
                     /gene="emrB"
                     /locus_tag="OO249_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5634541.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWECGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9914..10429)
                     /gene="luxS"
                     /locus_tag="OO249_000104"
     CDS             complement(9914..10429)
                     /gene="luxS"
                     /locus_tag="OO249_000104"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5634542.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10579..12135)
                     /gene="gshA"
                     /locus_tag="OO249_000105"
     CDS             complement(10579..12135)
                     /gene="gshA"
                     /locus_tag="OO249_000105"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5634543.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTQATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGKAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(12208..12636)
                     /locus_tag="OO249_000106"
     CDS             complement(12208..12636)
                     /locus_tag="OO249_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5634544.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12633..13199)
                     /gene="yqaB"
                     /locus_tag="OO249_000107"
     CDS             complement(12633..13199)
                     /gene="yqaB"
                     /locus_tag="OO249_000107"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5634545.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLAPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13347..13423)
                     /locus_tag="OO249_000108"
     tRNA            complement(13347..13423)
                     /locus_tag="OO249_000108"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13387..13389),aa:Arg,seq:acg)
     gene            complement(13622..13698)
                     /locus_tag="OO249_000109"
     tRNA            complement(13622..13698)
                     /locus_tag="OO249_000109"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13662..13664),aa:Arg,seq:acg)
     gene            complement(13896..13972)
                     /locus_tag="OO249_000110"
     tRNA            complement(13896..13972)
                     /locus_tag="OO249_000110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13936..13938),aa:Arg,seq:acg)
     gene            complement(13976..14068)
                     /locus_tag="OO249_000111"
     tRNA            complement(13976..14068)
                     /locus_tag="OO249_000111"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14032..14034),aa:Ser,seq:gct)
     gene            complement(14384..14569)
                     /gene="csrA"
                     /locus_tag="OO249_000112"
     CDS             complement(14384..14569)
                     /gene="csrA"
                     /locus_tag="OO249_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5634546.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14804..17434)
                     /gene="alaS"
                     /locus_tag="OO249_000113"
     CDS             complement(14804..17434)
                     /gene="alaS"
                     /locus_tag="OO249_000113"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5634547.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKDANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEVAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17562..18062)
                     /gene="recX"
                     /locus_tag="OO249_000114"
     CDS             complement(17562..18062)
                     /gene="recX"
                     /locus_tag="OO249_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5634548.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYDEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(18305..19366)
                     /gene="recA"
                     /locus_tag="OO249_000115"
     CDS             complement(18305..19366)
                     /gene="recA"
                     /locus_tag="OO249_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5634549.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19446..19943)
                     /gene="pncC"
                     /locus_tag="OO249_000116"
     CDS             complement(19446..19943)
                     /gene="pncC"
                     /locus_tag="OO249_000116"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5634550.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(20088..21173)
                     /gene="mltB"
                     /locus_tag="OO249_000117"
     CDS             complement(20088..21173)
                     /gene="mltB"
                     /locus_tag="OO249_000117"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5634551.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21429..21992
                     /gene="srlA"
                     /locus_tag="OO249_000118"
                     /gene_synonym="gutA"
     CDS             21429..21992
                     /gene="srlA"
                     /locus_tag="OO249_000118"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR5634552.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSQGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLALSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21989..22948
                     /gene="srlE"
                     /locus_tag="OO249_000119"
                     /gene_synonym="gutE"
     CDS             21989..22948
                     /gene="srlE"
                     /locus_tag="OO249_000119"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5634553.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            22959..23330
                     /gene="srlB"
                     /locus_tag="OO249_000120"
                     /gene_synonym="gutB"
     CDS             22959..23330
                     /gene="srlB"
                     /locus_tag="OO249_000120"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5634554.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23334..24113
                     /gene="srlD"
                     /locus_tag="OO249_000121"
     CDS             23334..24113
                     /gene="srlD"
                     /locus_tag="OO249_000121"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5634555.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            24218..24576
                     /gene="gutM"
                     /locus_tag="OO249_000122"
                     /pseudo
     CDS             24218..24576
                     /gene="gutM"
                     /locus_tag="OO249_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            24643..25416
                     /gene="srlR"
                     /locus_tag="OO249_000123"
                     /gene_synonym="gutR"
     CDS             24643..25416
                     /gene="srlR"
                     /locus_tag="OO249_000123"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5634556.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25409..26374
                     /gene="gutQ"
                     /locus_tag="OO249_000124"
     CDS             25409..26374
                     /gene="gutQ"
                     /locus_tag="OO249_000124"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5634557.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26371..27885)
                     /gene="norR"
                     /locus_tag="OO249_000125"
     CDS             complement(26371..27885)
                     /gene="norR"
                     /locus_tag="OO249_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5634558.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            28072..29511
                     /gene="norV"
                     /locus_tag="OO249_000126"
     CDS             28072..29511
                     /gene="norV"
                     /locus_tag="OO249_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5634559.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCRVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            29508..30641
                     /gene="norW"
                     /locus_tag="OO249_000127"
                     /pseudo
     CDS             29508..30641
                     /gene="norW"
                     /locus_tag="OO249_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
     gene            complement(30769..33021)
                     /gene="hypF"
                     /locus_tag="OO249_000128"
     CDS             complement(30769..33021)
                     /gene="hypF"
                     /locus_tag="OO249_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5634560.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAQVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(33174..33701)
                     /gene="hydN"
                     /locus_tag="OO249_000129"
     CDS             complement(33174..33701)
                     /gene="hydN"
                     /locus_tag="OO249_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5634561.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33850..34860)
                     /gene="ascG"
                     /locus_tag="OO249_000130"
     CDS             complement(33850..34860)
                     /gene="ascG"
                     /locus_tag="OO249_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5634562.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            35120..36577
                     /gene="ascF"
                     /locus_tag="OO249_000131"
     CDS             35120..36577
                     /gene="ascF"
                     /locus_tag="OO249_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR5634563.1"
                     /translation="MAKNYAALARSEIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTPRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYSIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            36586..38010
                     /gene="ascB"
                     /locus_tag="OO249_000132"
     CDS             36586..38010
                     /gene="ascB"
                     /locus_tag="OO249_000132"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR5634564.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(38169..38639)
                     /gene="hycI"
                     /locus_tag="OO249_000133"
     CDS             complement(38169..38639)
                     /gene="hycI"
                     /locus_tag="OO249_000133"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR5634565.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(38632..39042)
                     /gene="hycH"
                     /locus_tag="OO249_000134"
     CDS             complement(38632..39042)
                     /gene="hycH"
                     /locus_tag="OO249_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR5634566.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(39039..39806)
                     /gene="hycG"
                     /locus_tag="OO249_000135"
     CDS             complement(39039..39806)
                     /gene="hycG"
                     /locus_tag="OO249_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR5634567.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            complement(39806..40348)
                     /gene="hycF"
                     /locus_tag="OO249_000136"
     CDS             complement(39806..40348)
                     /gene="hycF"
                     /locus_tag="OO249_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR5634568.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(40358..42067)
                     /gene="hycE"
                     /locus_tag="OO249_000137"
     CDS             complement(40358..42067)
                     /gene="hycE"
                     /locus_tag="OO249_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5634569.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKEQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(42085..43008)
                     /gene="hycD"
                     /locus_tag="OO249_000138"
     CDS             complement(42085..43008)
                     /gene="hycD"
                     /locus_tag="OO249_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5634570.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITSRITWAGFGFAFLAFVSLLAA"
     gene            complement(43011..44837)
                     /gene="hycC"
                     /locus_tag="OO249_000139"
     CDS             complement(43011..44837)
                     /gene="hycC"
                     /locus_tag="OO249_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5634571.1"
                     /translation="MSAISLINSGVVWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILILSLLGGNAPLWWGIVLLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(44834..45445)
                     /gene="hycB"
                     /locus_tag="OO249_000140"
     CDS             complement(44834..45445)
                     /gene="hycB"
                     /locus_tag="OO249_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5634572.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNVITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(45570..46031)
                     /gene="hycA"
                     /locus_tag="OO249_000141"
     CDS             complement(45570..46031)
                     /gene="hycA"
                     /locus_tag="OO249_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5634573.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            46243..46593
                     /gene="hypA"
                     /locus_tag="OO249_000142"
     CDS             46243..46593
                     /gene="hypA"
                     /locus_tag="OO249_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5634574.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            46597..47469
                     /gene="hypB"
                     /locus_tag="OO249_000143"
     CDS             46597..47469
                     /gene="hypB"
                     /locus_tag="OO249_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5634575.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            47460..47732
                     /gene="hypC"
                     /locus_tag="OO249_000144"
     CDS             47460..47732
                     /gene="hypC"
                     /locus_tag="OO249_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5634576.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            47732..48853
                     /gene="hypD"
                     /locus_tag="OO249_000145"
     CDS             47732..48853
                     /gene="hypD"
                     /locus_tag="OO249_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5634577.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            48850..49860
                     /gene="hypE"
                     /locus_tag="OO249_000146"
     CDS             48850..49860
                     /gene="hypE"
                     /locus_tag="OO249_000146"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5634578.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            49934..52012
                     /gene="flhA"
                     /locus_tag="OO249_000147"
                     /pseudo
     CDS             49934..52012
                     /gene="flhA"
                     /locus_tag="OO249_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(52049..52402)
                     /locus_tag="OO249_000148"
     CDS             complement(52049..52402)
                     /locus_tag="OO249_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5634579.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(52479..52613)
                     /locus_tag="OO249_000149"
     CDS             complement(52479..52613)
                     /locus_tag="OO249_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5634580.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            52689..55250
                     /gene="mutS"
                     /locus_tag="OO249_000150"
     CDS             52689..55250
                     /gene="mutS"
                     /locus_tag="OO249_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5634581.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            55356..56012
                     /gene="pphB"
                     /locus_tag="OO249_000151"
     CDS             55356..56012
                     /gene="pphB"
                     /locus_tag="OO249_000151"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCR5634582.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMELDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(56063..56830)
                     /locus_tag="OO249_000152"
     CDS             complement(56063..56830)
                     /locus_tag="OO249_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5634583.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            57026..57934
                     /locus_tag="OO249_000153"
     CDS             57026..57934
                     /locus_tag="OO249_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5634584.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRVGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGDAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            57931..59193
                     /locus_tag="OO249_000154"
     CDS             57931..59193
                     /locus_tag="OO249_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5634585.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            59190..59828
                     /locus_tag="OO249_000155"
     CDS             59190..59828
                     /locus_tag="OO249_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5634586.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTVLSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            59833..60607
                     /locus_tag="OO249_000156"
                     /pseudo
     CDS             59833..60607
                     /locus_tag="OO249_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
     gene            60696..62060
                     /locus_tag="OO249_000157"
     CDS             60696..62060
                     /locus_tag="OO249_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR5634587.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(62154..63146)
                     /gene="rpoS"
                     /locus_tag="OO249_000158"
     CDS             complement(62154..63146)
                     /gene="rpoS"
                     /locus_tag="OO249_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5634588.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(63209..64300)
                     /gene="nlpD"
                     /locus_tag="OO249_000159"
     CDS             complement(63209..64300)
                     /gene="nlpD"
                     /locus_tag="OO249_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5634589.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSY
                     SGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGG
                     NAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTA
                     PVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAI
                     IATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMG
                     STGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(64440..65066)
                     /gene="pcm"
                     /locus_tag="OO249_000160"
     CDS             complement(64440..65066)
                     /gene="pcm"
                     /locus_tag="OO249_000160"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5634590.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(65060..65821)
                     /gene="surE"
                     /locus_tag="OO249_000161"
     CDS             complement(65060..65821)
                     /gene="surE"
                     /locus_tag="OO249_000161"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5634591.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(65802..66851)
                     /gene="truD"
                     /locus_tag="OO249_000162"
     CDS             complement(65802..66851)
                     /gene="truD"
                     /locus_tag="OO249_000162"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5634592.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     AAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQRKALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(66848..67327)
                     /gene="ispF"
                     /locus_tag="OO249_000163"
     CDS             complement(66848..67327)
                     /gene="ispF"
                     /locus_tag="OO249_000163"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5634593.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(67327..68037)
                     /gene="ispD"
                     /locus_tag="OO249_000164"
     CDS             complement(67327..68037)
                     /gene="ispD"
                     /locus_tag="OO249_000164"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5634594.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(68056..68367)
                     /gene="ftsB"
                     /locus_tag="OO249_000165"
     CDS             complement(68056..68367)
                     /gene="ftsB"
                     /locus_tag="OO249_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5634595.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(68561..68884)
                     /locus_tag="OO249_000166"
     CDS             complement(68561..68884)
                     /locus_tag="OO249_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5634596.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(68934..69539)
                     /gene="cysC"
                     /locus_tag="OO249_000167"
     CDS             complement(68934..69539)
                     /gene="cysC"
                     /locus_tag="OO249_000167"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5634597.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(69539..70966)
                     /gene="cysN"
                     /locus_tag="OO249_000168"
     CDS             complement(69539..70966)
                     /gene="cysN"
                     /locus_tag="OO249_000168"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5634598.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     PSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(70968..71876)
                     /gene="cysD"
                     /locus_tag="OO249_000169"
     CDS             complement(70968..71876)
                     /gene="cysD"
                     /locus_tag="OO249_000169"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5634599.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            72128..73165
                     /gene="iap"
                     /locus_tag="OO249_000170"
     CDS             72128..73165
                     /gene="iap"
                     /locus_tag="OO249_000170"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5634600.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYISQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTTEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(73372..73665)
                     /gene="cas2"
                     /locus_tag="OO249_000171"
     CDS             complement(73372..73665)
                     /gene="cas2"
                     /locus_tag="OO249_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR5634601.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(73665..74585)
                     /gene="cas1e"
                     /locus_tag="OO249_000172"
     CDS             complement(73665..74585)
                     /gene="cas1e"
                     /locus_tag="OO249_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCR5634602.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLQVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(74582..75232)
                     /gene="cas6e"
                     /locus_tag="OO249_000173"
     CDS             complement(74582..75232)
                     /gene="cas6e"
                     /locus_tag="OO249_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR5634603.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDISVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(75214..75960)
                     /gene="cas5e"
                     /locus_tag="OO249_000174"
     CDS             complement(75214..75960)
                     /gene="cas5e"
                     /locus_tag="OO249_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCR5634604.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSLSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(75971..77049)
                     /gene="cas7e"
                     /locus_tag="OO249_000175"
                     /pseudo
     CDS             complement(75971..77049)
                     /gene="cas7e"
                     /locus_tag="OO249_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            complement(77077..77625)
                     /gene="casB"
                     /locus_tag="OO249_000176"
     CDS             complement(77077..77625)
                     /gene="casB"
                     /locus_tag="OO249_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCR5634605.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLEEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALGAGRWALGAGILPGWRRR"
     gene            complement(77622..79184)
                     /gene="casA"
                     /locus_tag="OO249_000177"
     CDS             complement(77622..79184)
                     /gene="casA"
                     /locus_tag="OO249_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HCR5634606.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRSWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATFIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPDELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            complement(79282..80836)
                     /gene="cas3"
                     /locus_tag="OO249_000178"
                     /pseudo
     CDS             complement(79282..80836)
                     /gene="cas3"
                     /locus_tag="OO249_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
BASE COUNT        19062 a        21957 c        20661 g        19156 t
ORIGIN      
        1 tgtgatgggg aaagcggttg aaacagaaga tgaagactgg aatttctgag ggtgttattt
       61 tcaaaaatat cactacccgc agcagggaaa taattcccgc caaatagctt tttatcacgc
      121 aaatattctg tagtgatcta cactgagact ctgttgcatt attcgcctga aactacaata
      181 ttcaggcgtt ttttcgctat ctatgacaaa aaatatcaac ttttctcgat ttgctctcag
      241 cccttatatc acgggaaatt ccggcgattt gctcgcatca atattcatgc cacatttgcc
      301 ctcaggggtt gcctcagatt ctcagtatgt tagggtagaa aaaagtgact atttccattg
      361 ggtaatatat cgacataaac aaataaagga atctttctat tgcatggcaa ttaaattaga
      421 aattaaaaat ctttataaaa tatttggcga gcatccacag cgagcgttca aatatatcga
      481 acaaggactt tcgaaagaac aaattctgga aaaaactggg ctatcgcttg gcgtaaaaga
      541 cgccagtctg gccattgaag aaggcgagat atttgtcatc atgggattat ccggctcggg
      601 taaatccaca atggtacgcc ttctcaatcg cctgattgaa cccacccgcg ggcaagtgct
      661 gattgatggt gtggatattg ccaaaatatc cgatgccgaa ctccgtgagg tgcgcagaaa
      721 aaagattgcg atggtcttcc agtcctttgc cttaatgccg catatgaccg tgctggacaa
      781 tactgcgttc ggtatggaat tggccggaat taatgccgaa gaacgccggg aaaaagccct
      841 tgatgcactg cgtcaggtcg ggctggaaaa ttatgcccac agctacccgg atgaactctc
      901 tggcgggatg cgtcaacgtg tgggattagc ccgcgcgtta gcgattaatc cggatatatt
      961 attaatggac gaagccttct cggcgctcga tccattaatt cgcaccgaga tgcaggatga
     1021 gctggtaaaa ttacaggcga aacatcagcg caccattgtc tttatttccc acgatctcga
     1081 tgaagctatg cgtattggcg accgaattgc cattatgcaa aatggtgaag tggtacaggt
     1141 cggcacaccg gatgaaattc tcaataatcc ggcgaatgat tatgtccgta ccttcttccg
     1201 tggcgttgat attagtcagg tattcagtgc gaaagatatt gcccgccgga caccgaatgg
     1261 cttaattcgt aaaacccctg gcttcggccc acgttcggca ctgaaattat tgcaggatga
     1321 agatcgcgaa tatggctacg ttatcgaacg cggtaataag tttgtcggcg cagtttccat
     1381 cgattcgctt aaaaccgcgt taacgcagca gcaaggtctt gatgcggcgc tgattgatgc
     1441 gccgttagca gtcgatgcac aaacgcctct tagcgagttg ctctctcatg tcggacaggc
     1501 accctgtgcg gtacccgtgg tcgacgagga ccaacagtat gtcggcatca tttcgaaagg
     1561 aatgctgctg cgcgctttag atcgtgaggg ggtaaataat ggctgatcaa aataatccgt
     1621 gggataccac gccagcggcg gacagtgccg tgcaatccgc agacgcctgg ggtacaccgg
     1681 cgactgcacc gactgacggc ggtggtgctg actggctgac cagtacgcct gcgccaaacg
     1741 tcgagcattt taatattctc gatccgttcc ataaaacgct gatcccgctc gacagttggg
     1801 tcactgaagg gatcgactgg gttgttaccc atttccgtcc cgtcttccag ggcgtgcgcg
     1861 ttccggttga ttatatcctc aacggtttcc agcaagtgct gctgggtatg cccgcaccgg
     1921 tggcgattat cgttttcgct ctcatcgcct ggcagatttc cggggtcgga atgggcgtgg
     1981 cgacgctggt ttcgctgatt gccatcggcg caatcggtgc ctggtcgcag gccatggtta
     2041 ccctggcgct ggtgttaacc gccctgctgt tctgtatcgt cattggtttg ccgctgggga
     2101 tctggctggc gagaagtccg cgagcggcga aaattattcg tccactgctt gatgccatgc
     2161 agaccacgcc agcgtttgtt tatctggtgc caatcgtcat gctatttggt atcggtaacg
     2221 tgccgggcgt ggtggtgacg atcatctttg ctctgccgcc gattatccgt ctgaccattc
     2281 tggggattaa ccaggttccg gcggatctga ttgaagcctc gcgctcattc ggtgccagcc
     2341 cgcgccagat gctgttcaaa gttcagttac cgctggcgat gccgagcatt atggcgggcg
     2401 ttaaccagac gctgatgctg gccctttcta tggtggtcat tgcctcgatg attgccgtcg
     2461 gcgggctggg tcagatggta cttcgcggta tcggtcgtct ggatatgggg cttgccaccg
     2521 ttggcggcgt cgggattgtg atcctcgcca ttatcctcga tcgcctgacg caggccgttg
     2581 ggcgcgactc acgcagtcgc ggcaaccgtc gctggtacac cactggccct gttggtctgc
     2641 tgacccgccc attcattaag taactctgca cttgcccggt gacgccgggc attatcaccc
     2701 tgccaaaaaa aggaataaca atgcgacata gcgtactttt tgcgacagcg tttgccacgc
     2761 ttatctctac acaaactttt gctgccgatc tgccgggcaa aggtattact gttaatccag
     2821 ttcaaagcac catcaccgaa gaaaccttcc agacgctgct ggtcagccgt gcgctggaga
     2881 aattaggcta taccgtcaac aaacccagcg aagtagatta caacgttggc tacaccttgc
     2941 ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac gccactgcat gacaacatgt
     3001 acgaagctgc cggtggcgat aagaaatttt atcgtgaagg ggtatttgtt aacggcgcgg
     3061 cacagggtta cctgatcgat aagaaaaccg ccgaccagta caaaatcacc aacatcgcac
     3121 aactgaaaga gccgaagatc gccaaactgt tcgataccaa cggcgacggt aaagcggatt
     3181 taaccggttg taatcctggc tggggctgcg aaggtgcgat caaccaccag cttgccgcgt
     3241 atgaactgac caacaccgtg acgcataatc aggggaacta cgcagcgatg atggccgaca
     3301 ccatcagtcg ctacaaagag ggcaaaccgg tgttttatta cacctggacg ccgtactggg
     3361 tgagtaatga gctgaagcca gggaaagatg tggtctggtt gcaggtgccg ttctccgcac
     3421 tgccgggcga taaaaatgcc gataccaaac tgccgaatgg cgcgaattac ggcttcccgg
     3481 tcagcaccat gcatatcgtt gccaacaaag cctggaccga gaaaaatccg gcggcagcca
     3541 aactgtttgc cattatgcag ttgccagtgg ccgatattaa cgcccagaac gccattatgc
     3601 atgacggcaa agcctcagaa ggcgatattc agggccacgt tgatggctgg atcaaagccc
     3661 accagcagca gttcgatggc tgggtgaatg aggcgctggc agcgctgaag taatttttat
     3721 tcaggcggat aaggcgtttt tgccttatcc gccgctctgt acacaatgcc tgatgcgacg
     3781 ctgacgcgtc ttatcatgcc tacaaaatac actcattccg tatggcggat aaggcgtttt
     3841 cgccgcatcc gccgttctgt gcacaatgcc tgatgcgacg ctggcgcgtc ttatcatgcc
     3901 tacaaaccac atcgcacatt tcaacaagct atctttcatc ccatattcat caacatccgc
     3961 tattattgat ttccagctta atcatcacct gatgaacaaa aataatgact aaacctaatc
     4021 atgagcttag cccggcgctg atcgtgctga tgtctatcgc caccggtctg gcggtagcca
     4081 gtaactatta cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc ctttccgcca
     4141 gttcggcagg ctttattgtt accgccgcgc aattgggcta tgcagcaggt ctgctgtttc
     4201 ttgttcccct cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg accttactgg
     4261 cggcaggcgg catgttaatc actgccagca gtcagtcgct ggcgatgatg atcctcggta
     4321 cggcattaac cggtttattc tctgtcgtgg cacaaattct ggttccgctg gcagcgacgc
     4381 tggcttcacc ggacaaacgc ggcaaagtgg ttggcactat tatgagcggt ctgctgttgg
     4441 ggatcttact cgcacggacc gttgccggat tgctggcgaa tctcggcggc tggcgcaccg
     4501 tcttttgggt tgcctcggtg ttaatggcac tgatggcgct ggcattatgg cgtggtctgc
     4561 cacaaatgaa atcagaaacc cacctcaact acccacagtt gttaggttcc gttttcagca
     4621 tgtttatcag cgataagatc ctgcgccccc gcgcattgct gggctgcctg acctttgcca
     4681 acttcagcat tctctggacc tcaatggcct ttttgcttgc cgctccacct tttaactaca
     4741 gcgatggtgt cattggtctg tttggacttg cgggagctgc cggagcgttg ggcgctcgtc
     4801 cggcgggcgg ttttgccgat aagggtaaat cgcaccacac cacaactttc ggtctgctgc
     4861 tgctattact ttcatggctg gcgatctggt ttggtcacac ttccgtactg gcgctgatta
     4921 tcggcatcct ggtgctggat ctcaccgtac agggcgtgca tatcactaac cagacggtaa
     4981 tttatcgaat acaccctgat gcgcgtaatc gcctgaccgc aggttacatg accagctact
     5041 ttattggcgg tgccgccggt tcgctaattt cagcctcagc ctggcaacat ggcggttggg
     5101 ctggcgtttg tctggctggc gcgacgattg ccctggttaa cttactggtc tggtggcgag
     5161 gttttcatcg tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt ggctttacac
     5221 ttataagggt gttaagaagc ccatcagtct gataaggtta agatattcat tcagtctatt
     5281 tataatatta acaatcgtta agcgtacact ctatggaaag ccctactcca cagcctgctc
     5341 ttggttcggc gaccttcatg gaaggatgca aagacagttt accgattgtt attagttata
     5401 ttccggtggc ctttgcgttc ggtctgaatg cgacccgtct gggattctct cctctcgaaa
     5461 gcgttttttt ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt accgcgatgc
     5521 tggcagccgg gagtagtttg tgggttgctg cactgaccgt catggcaatg gatgttcgcc
     5581 atgtgttgta tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa aaatcgaaaa
     5641 ccgccctgtg ggcatttggc ctgacggatg aggtttttgc cgccgcaacc gcaaaactgg
     5701 tacgcaataa tcgccgctgg agcgagaact ggatgatcgg cattgccttc agttcatggt
     5761 catcgtgggt atttggtacg gtaatagggg cattctccgg cagcggcttg ctgcaaggtt
     5821 atcccgccgt tgaagctgca ttaggtttta tgcttccggc actctttatg agtttcctgc
     5881 tcgcctcttt ccagcgcaaa caatctcttt gcgttaccgc agcgttagtt ggtgcccttg
     5941 caggcgtaac gctattttct attcccgtcg ccattctggc aggcattgtc tgtggctgcc
     6001 tcactgcgtt aatccaggca ttctggcaag gagcgcccga tgagctatga ggttctgctg
     6061 cttgggttac tggttggcgt ggcgaattat tgcttccgct atttgccgct gcgcctgcgt
     6121 gtgggtaatg cccgcccaac caaacgtggc gcggtaggta ttttgctcga caccattggc
     6181 atcgcctcga tatgcgctct gctggttgtc tctaccgcac cagaagtgat gcacgataca
     6241 cgccgtttcg tgcccacgct ggtcggcttc gcggtactgg gtgccagttt ctataaaaca
     6301 cgcagcatta tcatcccaac actgcttagt gcgctggcct atgggctcgc ctggaaagtg
     6361 atggcgatta tataacccac aagaatcatt tttctaaaac aatacattta ctttatttgt
     6421 tactgtcgtt actatatcgg ctgaaattaa tgaggtcata cccaaatgga tagttcgttt
     6481 acgcccattg aacaaatgct aaaatttcgc gccagccgcc acgaagattt tccttatcag
     6541 gagatccttc tgactcgtct ttgcatgcac atgcaaagca agctgctgga gaaccgcaat
     6601 aaaatgctga aggctcaggg gattaacgag acgttgttta tggcgttgat tacgctggag
     6661 tctcaggaaa accacagtat tcagccttct gaattaagtt gtgctcttgg atcatcccgt
     6721 accaacgcga cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga acgtcgtgaa
     6781 agcgataacg atcgccgctg cctgcatctg caattaacgg aaaaaggtca cgagtttttg
     6841 cgcgaggttt taccaccgca gcataactgc ctgcatcaac tctggtccgc gctcagcaca
     6901 acagaaaaag atcagctcga gcaaatcacc cgcaaattgc tctcccgtct cgaccagatg
     6961 gaacaagacg gtgtggttct cgaagcgatg agctaacgcg tcatctcgct caaaaatcca
     7021 gatttataaa agaaaaaatg accggccagc atcgcaacat gctggccttt ttggcaagca
     7081 ggtcggctca gccgatgagt taagaagatc gtggagaaca atatgagcgc aaatgcggag
     7141 actcaaaccc cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct gctcctcctt
     7201 ctcaccttgc tctttataat tattgccgta gcgataggga tttattggtt tttggtactg
     7261 cgtcatttcg aagaaaccga tgacgcatac gtggcaggga atcagattca aattatgtct
     7321 caggtgtctg gcagcgtgac gaaagtctgg gccgataaca ccgattttgt aaaagaaggc
     7381 gatgttctgg tcactctcga cccgacagat gctcgccagg cgtttgaaaa agccaaaact
     7441 gcactggctt ccagcgttcg ccaaacccac cagctgatga ttaacagcaa acagttacag
     7501 gcgaatattg aggtgcaaaa aatcgccctc gcgaaagcac aaagcgacta caaccgccgt
     7561 gtgccgctgg gcaatgccaa cctgattggt cgcgaagagc tgcaacacgc ccgcgacgcc
     7621 gtcaccagtg cccaggcgca actggacgtc gctattcaac aatacaatgc caatcaggcg
     7681 atgattctgg ggactaaact ggaagatcag cctgccgtgc aacaggctgc caccgaagta
     7741 cgtaacgcct ggctggcgct ggagcgtacc cgtattgtca gtccgatgac cggttatgtc
     7801 tcccgccgcg cggtacagcc tggggcgcaa attagcccaa cgacgccgct gatggcggtc
     7861 gttccagcca ccaatatgtg ggtggatgcc aactttaaag agacgcagat tgccaatatg
     7921 cgtatcggtc agccggtcac tatcaccacg gatatttacg gcgatgatgt gaaatacacc
     7981 ggtaaagtgg ttggtctgga tatgggcaca ggtagcgcat tctcactgct tccggcgcaa
     8041 aatgcgaccg gtaactggat caaagtcgtt cagcgtctac ctgtgcgtat cgaactggac
     8101 cagaaacagc tggagcaata cccgctgcgc attggtttgt ccacgctggt gagcgtcaat
     8161 accactaacc gtgacggtca ggtactggca aataaagtac gttcaacacc ggtcgcggta
     8221 agtaccgtgc gtgaaatcag cctggcacct gtcaataaac tgatcgacga tatcgtaaaa
     8281 gctaacgctg gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc tggaaggcgc
     8341 gcaactggtc attatgacga ttgcgctgtc actggcgaca ttcatgcagg tgctggactc
     8401 caccattgct aacgtggcga tccccactat cgccgggaat ctgggctcat cgctcagcca
     8461 gggaacgtgg gtaatcactt ctttcggggt ggcgaatgcc atctcgatcc cgcttaccgg
     8521 ctggctggca aagcgcgtcg gggaagtgaa actgttcctt tggtccacca tcgcctttgc
     8581 tattgcgtcg tgggagtgtg gtgtctccag cagcctgaat atgctgatct tcttccgtgt
     8641 gattcagggg attgtcgctg ggccgttgat cccactttcg caaagtcttt tgctgaataa
     8701 ttacccgcca gccaaacgct caatcgcgct ggcgttgtgg tcgatgacgg tgattgtcgc
     8761 gccaatttgc ggcccaatcc tcggcggtta tatcagcgat aattaccact ggggctggat
     8821 attcttcatc aacgtgccaa ttggcgtggc ggtggtgttg atgacactgc aaaccctgcg
     8881 cggacgcgaa acccgcaccg aacggcggcg gattgatgcc gtggggctgg cactgctggt
     8941 tattggtatc ggtagcctgc agattatgct cgaccgcggt aaagagctgg actggttttc
     9001 atcacaggaa attatcattc ttaccgtggt ggcggtggtg gctatctgct tcctgattgt
     9061 ctgggagctg accgacgata acccgatagt cgatctgtcg ttgtttaagt cgcgcaactt
     9121 caccatcggc tgcttgtgta tcagcctcgc gtatatgctc tacttcggcg cgattgttct
     9181 gctgccgcag ttgttgcagg aggtctacgg ttacacggcg acctgggcag gtttggcctc
     9241 tgcgccggta gggattattc cggtgatcct gtcgccgatt atcggccgct tcgcgcataa
     9301 actggatatg cggcggctgg taaccttcag ctttattatg tatgccgtct gcttctactg
     9361 gcgtgcctat acctttgaac caggtatgga ttttggcgcg tcggcctggc cgcagtttat
     9421 ccaggggttt gcggtggcct gcttctttat gccgctgacc accattacgc tgtctggttt
     9481 gccaccggaa cgactggcgg cggcatcgag cctctctaac tttacgcgaa cgctggcggg
     9541 gtctatcggc acgtcgataa ccacgaccat gtggaccaac cgcgagtcga tgcaccatgc
     9601 gcagttgact gagtcggtaa acccgttcaa cccgaatgcc caggcgatgt acagtcaact
     9661 ggaagggctt gggatgacgc aacagcaggc atcaggctgg attgcccagc agatcactaa
     9721 tcaggggctg attatttccg ccaatgagat cttctggatg tcagccggga tattcctcgt
     9781 cctgctgggg ctggtgtggt ttgctaaacc gccctttggc gcaggtggcg gcggaggtgg
     9841 tgcgcactaa gtacaactaa gccagttcat ttgaactggc ttttttcaat taattgtgaa
     9901 gatagtttac tgactaaatg tgcagttcct gcaacttctc tttcggcagt gccagttctt
     9961 cgttgctgtt gatgcgtacg tcacgttcca gaatgctacg cgcaatatcc tgcgcttcct
    10021 gcaacgagtg catctggtaa gtgccacact ggtagacgtt cagctccggg atctgattct
    10081 gatcctgcac tttcagcacg tcttccattg ccgctttcca ggcatcagca acacgctgct
    10141 catctggcgt accaatcaga ctcatataaa aaccggtgcg gcagcccatt ggcgagatat
    10201 cgataatctc tacaccatta ccgttaagat ggttacgcat aaaaccagca aacaggtgct
    10261 ccagagtatg gctccctctt tctggcatca cttctttgtt cggcacgcag aagcgcagat
    10321 cgaacacggt gattgcgtcg ccatgcgggg tgttcattgt tttcgccacc cgaactgcag
    10381 gcgcttccat ccgggtatga tcgactgtga agctatctaa caacggcatt tagccacctc
    10441 cggtaatttt tttaaaaatt ttctgaactc tttcttccca ggcgagtctg agtatatgaa
    10501 agacgcgcat ttgttatcat catccctgaa ttcagagatg aaattttggc cactcacgag
    10561 tggccttttt cttttctgtc aggcgtgttt ttccagccac accgcaaacg gttcggtatc
    10621 agcggtttcc atttcctgct gacggcgttc agatgcctcg cgctcggcta caaaatcctc
    10681 ttcgcgcaga atttccaacg gctcttcacg cagcagatta cggtaggctt ctgcaaacgc
    10741 tttgcctgtt ccgccaatac cagtatcaat catagacctt aagatacggg cagagaaagt
    10801 cagatcggga ttatcgaagc aggcgaccag ttcatcacac actttctgat acgctttgcc
    10861 gccgttaatg ctatccaacg tttgcgcgac gcgtttcaga tcgcggaaca gatctttacc
    10921 cacctgcggt aacgggaact gcgcggtttc gcagccgata cccagcgtca ggcccggttt
    10981 gcgaccttcg aggatcaccc ggttccagtt aacgcgtgta caggcaagtt cgctactgct
    11041 catttccggt gcatcagcca gcgcacacca gaccataaac aggtcgagga atcgcacctg
    11101 ctgttcatct acaccaatcg gcgagaacgg gttgatgtcc agcgaacgca cttcaatata
    11161 ttcaatgccg ccacgtaaca gcgcatcaga aggtgactcg ccgctgcggg taacgcgttt
    11221 tggacgaatc ggcgcataca gttcgttttc aatctgcagc acgttgctat tgatttgcag
    11281 cctcttaccg tctttctcaa taccaatctt cgcgtactct tccgatggcg ttttgattgc
    11341 ctgtttaagg cccgctacgt actcgtaaag atcgttgaag gtaataccaa gattgctttg
    11401 cgatttattg gtatagccga gatcgctcaa acgaagagag gtcgcatacg gcaggtaata
    11461 cataccgcac tcggttttct caaacggcag cgacgttggt tttccttgca ggaaagaaga
    11521 acaaatcgcc ggagatgcac caaacagata aggaatgacc caaccgaaac gatagtaatt
    11581 gcggataacg cggaaatagc ccgcagaaat tttctctttg gcatcagtgc ccgagatatc
    11641 accgcacttc gcttgccaga atgccattgg caaagagaaa ttgtagtgca cgccggaaat
    11701 ggtttgcatc agcgcgccgt agcgattttt cagcccttca cgatacagcg ttttaaagcg
    11761 tccggtgtta gaagtgccgt actgtgccag ttcgatgtcc tgaccttctg cgatgtagca
    11821 tggcatactt aacggccaca tccgctcatc gcccatattg cgcgccgtat aacgatgcag
    11881 atcgcgcata aaggtcaaca tatgttcaat atcaccatcc actggtgtaa tgaattccag
    11941 caatgcttcc gcaaaatcgg tagtaatcca tttgtgcgtc agtgcggaac ctaatgcttc
    12001 aggatgacct gttgttgcct gtgtgccatc agcattaaca cgcaaagttt cgcgctccag
    12061 cccacgctgt atccccttta acgcctgagg atgtttttcc agccaggcca gcgcctgtga
    12121 tacgtccggg atcaaattga cctcccgcct gtcaaaatcg ttttaattag cataactgta
    12181 atggtgacca tatgtgcagg cctacaatta gtgccaccac atcatgccct gaacggtagc
    12241 tgctgcaact gcaacatagc gtaaagcttt accaaggcac aaaaaaaaga ttaccggtcc
    12301 ccacgagatg cgcatccatc ccgctaacag acacagtaaa tcgccaacca cgggcatcca
    12361 gcttaataat agcgtgactg caccatagcg tttcagccag ccagtagctt tctcttgcca
    12421 gcgcgatgtt ttacgcaatg gaaagaaacg cccaaggata acgttagtta accctccaag
    12481 gctattaccc attgttgctg ttaaaactaa aacccaggga taactgatcc cggaaagcaa
    12541 cattgccacc agcacgactt cggagttgcc gggtaatagt gtagcgctga gaaaactact
    12601 ggcaaacaac gagaaaagcg ataacgcttc actcacagca agcgaacatc cacggcgtcc
    12661 atgcctgctg cacgggccgc ctgaataccg aaatcggcat cttcaaagac cacacactgc
    12721 gtcggttgca cgcccatacg ctgcgcgcac aacaaaaatg tgtctggcgc gggtttatgg
    12781 tgtttgacgt gatcagcagc gacgacggcg tcaaaataac ggcgtaatcc caggtgcgcc
    12841 agcaatgcct cagcgatggc gctttcactc cccgttccta cagccattgg gcgacgacca
    12901 tgccaacttt tcaccacttc aacaagagga agcggctcga cactatccag cagcatactt
    12961 cttactgctt ctgttttttc acgcgctaac gcatgcgggg cgagatcggc ctgattcagc
    13021 tcaataattg cctgagcaat acgccaggtg ggcgatccat taagcgcaat cattgcctga
    13081 acatcgtact gaagaccgta gtgccctaat acttcgcgcc acgctttacg gtgcgtaggc
    13141 tccgtatcca ggattgtgcc atccatatca aaaattaaac ctgcataacg ctcgtacatg
    13201 gtcttctcgc aagtcgaaaa atatgacgct actttagcgt aattgcttga ttttgtcgct
    13261 gatggcgaag gagaagaaaa ttcaggaaat ggataaagta gttatttaga aatgagatat
    13321 ttttgaagga aatttttggg agaagatggt gcatccggga ggattcgaac ctccgaccgc
    13381 tcggttcgta gccgagtact ctatccagct gagctacgga tgcatcggga aaatttattt
    13441 tactgctgat attgatatcg ctactaaagc catatcaagt aagagatggt gcatccggga
    13501 ggattactcg ccttgcggct cgcccttcgg gccgttgcta aagcaacgtt atcatcccta
    13561 gtgcctgcgg ttaattccgc tacctcttcg ataccttcat tgctgaagat ttcggagaat
    13621 atggtgcatc cgggaggatt cgaacctccg accgctcggt tcgtagccga gtactctatc
    13681 cagctgagct acggatgcat cgggaaactt actttactgc agattttttg ataccgctac
    13741 taaagccgta tcaagtaaga gatggtgcat tcgggaggat tactcgcctt gcggctcgcc
    13801 cttctggccg ttgctaaagc aacgttatcc tccctggtgc ctgcggttat accgccacct
    13861 cttccatacc ttcatcgctg aaagtacgta gaatatggtg catccgggag gattcgaacc
    13921 tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat gcaaatggcg
    13981 gtgaggcggg gattcgaacc ccggatgcag cttttgaccg catactccct tagcagggga
    14041 gcgccttcag cctctcggcc acctcaccac acgcctctta cgagtgcttc gaagaacttg
    14101 tttatcgctc atcgtcgctg cgtggcgcac atattacttt ctgagactta taagtcaaac
    14161 aatttttccc acacttttat cgtttgcaca cttcacgctc aattagtctg taaaaacgtc
    14221 aaaaagagtg ttttatctac agaaaaacgg aggtctggca gatagcagta atgcaaaaaa
    14281 atggagactt aagttgaatg aacgggagta aagcgaaaag actatagagt gaaggagaaa
    14341 ttttgagggt gcgtctcacc gataaagatg agacgcggaa agattagtaa ctggactgct
    14401 gggatttttc agcctggata cgctggtaga tctcttcacg gtgaacagaa acttccttcg
    14461 gggcatttac gccaatacgt acctggttgc ccttcacccc taaaactgtc acggtgacct
    14521 catccccaat catgagggtc tcaccaactc gacgagtcag aatcagcatt ctttgctcct
    14581 tgaaagatta aaagagtcgg gtctctctgt atcccggcat tatccatcat ataacgccaa
    14641 aaagtaagca atgacaaaca cattacatgt aagcagtcat ggcattacat tctgttaaac
    14701 ctaagtttag ccgatataca caacttcaac ctgactttat cgttgtcgat agcgttaatg
    14761 cgaatgccgt gaagcgagtc cacggcattg cctgacgctt atattattgc aatttcgcgc
    14821 tgacccagcc tttcacactg gctaacgctg caggtaaggc cgcagcatcc gtaccaccgg
    14881 cttgcgccat gtcaggacgt ccaccaccct tgccgcccac ctgctgagcg accataccaa
    14941 tcagttcccc tgctttcaca cgatctgtga cgtccttaga tacgcctgca atcagagaaa
    15001 ccttaccttc ggctaccgtt gccagcacga taattgtcga ccccagctga ttttttaaat
    15061 cgtcaaccat ggtacgcaac attttcggct caacaccgct aagctcgcta accaacagct
    15121 taacaccatt aacatcaatt gccttactgg aaagatttgc gctctcctgt gcggcagctt
    15181 gttctttaag ctgttgtagt tctttttcta actgacgcgt acgttccagt actgagcgca
    15241 ctttatccgc cagattattg ctatcgcctt tcagcagatg cgcgacttcg cttaagcgat
    15301 cgctgtctgc atgaacggtg gtgatagcgc cttctccggt taccgcttcg atacgacgaa
    15361 cgcctgcagc agtacccgat tcagagatga tgcggaacag accaatatca ccagtgcggc
    15421 tggcgtgagt accgccacac agctcggtgg agaaatcgcc catgctcagc acgcgtacac
    15481 gctcatcata cttctcgccg aacagcgcca tcgcaccttt cgctttcgcc acttcgagat
    15541 ccatgatgtt ggtttcgatc ggcaaattgc gacgaatctg tgcgttcacc aggtcttcga
    15601 ccgcacgaat ctcttccggt ttcatcgctt cgttgtgtga gaagtcgaag cgcagcacct
    15661 tgtcgttaac cagtgaacct ttctgcgata catgagtccc cagaacctgg cgcagcgcag
    15721 cgtgcatcag gtgcgttgcg gagtgattca gacgaatacg ggcgcgacga gcctcatcaa
    15781 catcagcctg cacagcgtcg cccactttca gagaacccgc agcaagttta ccgatgtgac
    15841 caatcgcctg gccgtatttc tgcgtatctt ccaccgcgaa ggagaagtta gcgtctttca
    15901 gttcgccttt atcaccaacc tgaccgccgg attccgcata gaatggtgtt tgatctagca
    15961 cgaccacagc ttcctggcct gcattgatgg catcaaccgc tttaccatca acaaacagcg
    16021 cggtcacttt gccgttcagt tccagatggt catagccttt aaattcagat gcactatcaa
    16081 cacggatcat tgcgttgtaa tcggcaccaa agccgctggc ttcgcgcgcg cgacgacgct
    16141 gctcttccat tgctgcttca aaaccagctt cgtcaacttt gatgttgcgc tcacgacaaa
    16201 catcagccgt caggtcaacc gggaagccat aggtgtcgta cagacggaaa gccgtttcac
    16261 catccagcgt atcaccagaa agttttgcca gctcttcatc cagcaacgcc agaccgcgct
    16321 ccagagtacg ggcaaactgc tcttcttcag tcttcagcac ctgctcaacc tgcgcctgct
    16381 ggcgtttcag gtcttcaccc gcagagccca taacgtcgat cagcggacca accagtttgt
    16441 agaagaaggt ttctttcgcg ccgagcatat tgccgtgacg cactgcgcga cgaatgatac
    16501 gacgcagtac ataaccacgg ttttcattgg acggcattac gccatccgcg atcaggaacg
    16561 cacaagaacg aatgtggtca gcgattacgc gcagcgattt attgctcaga tcggtcgcgc
    16621 cagtgacttt cgctaccgcc tggatcaacg tgcggaacag gtcgatgtca tagttagagt
    16681 taacgtgttg cagcaccgca gcaatacgct ccagacccat accggtatct acagacggct
    16741 tcggcagcgg ttccatcgtg ccatctgcct ggcggttgaa ctgcatgaag acgatgttcc
    16801 agatctcaat gtagcggtcg ccgtcttctt ccggacttcc cggagggcca ccccaaatgt
    16861 ggtcgccgtg atcgtagaag atttcggtgc acgggccgca cggaccagtg tcacccatct
    16921 gccagaagtt gtcagatgcg tatggcgcac ctttgttatc gccgatgcga ataatacgtt
    16981 cgcgcgggat cccgacttct ttttcccaga tttcgtaggc ttcgtcgtcg ctttcataga
    17041 cggtaaccca cagacgctct ttcggcaggg caaaccattt ttcgctggtc agcagttccc
    17101 atgcaaactg aatggcatcg tgtttgaaat agtcgccgaa gctgaagttg cccagcattt
    17161 cgaagaaggt atggtgacgc gcggtgtaac cgacgttttc caggtcgttg tgtttaccac
    17221 ccgcacgcac gcagcgttgg gaagtggtag cgcgggaata attacgcttg tcgagcccaa
    17281 ggaacacatc cttgaactga ttcatcccgg cgttggtaaa caacaaagtt gggtcgttat
    17341 ggggtaccag ggagctgctg gcaactacct gatgtccctt actatggaaa aagtcgagaa
    17401 acgcctgacg gatctcagcg gtgctcttgc tcataattat cctgaaatca agctaacgaa
    17461 atatcgccac cagctccagc gtgtcttaac cgccgggctg gtaactgaaa agtgggaata
    17521 agataagttt tcttgactgg gaagtaaaat accgtatgcg ttcagtcggc aaaatttcgc
    17581 caaatatcct ggatatcttc catcagatag ccacgataga gcagaaaacg ctggatctta
    17641 actttttctg aaaagacagt tggcaaaggt tcatcatatt ttcgcgtcgc ctgatcgcgc
    17701 gccagtgcgc accagtcgat gtcacattca cgcatcgctt tttctgtcgc ttcgcgggaa
    17761 atacctttct gattcagttc ctgacgaata cgtgcaggtc cataaccttt gcggctacgg
    17821 ctggcgataa agcgcgcaac aaatcggctg tcatcgagat agccatgttc atggcaccat
    17881 gcaataacgc gctcgtaatc ttctgccgta gcatcaatct cttctgggcc atttttgccc
    17941 ataatcggtg ccgcgagttt acgtcgcagt tcttgctcac tgtgatcgcg caccgccaga
    18001 atgcgtaccg cacgatccaa caggcgagca tatgccgggc gacgggacgt tgattctgtc
    18061 atggcatatc cttacaactt aaaaaagcaa aagggccgca gatgcgaccc aggttttgct
    18121 gataaacaga tttaatagtc aatgagtgtg cagaatgttc ccgcaggccc aattcgttag
    18181 aggatcgagg tgagagtaat ttttcacgcc agtgctggcc tgttcccctc accctaaccc
    18241 tctccccaaa ggggcgaggg gaccgtccgt gcacacatta tctattatca tcaataacga
    18301 acgattaaaa atcttcgtta gtttctgcta cgccttcgct gtcatctaca gagaaatccg
    18361 gcgttgagtt cgggttgctc agcagcaact cacgtacttt cttctcgatc tctttcgcgg
    18421 tttccgggtt atctttcagc caggcagttg cattcgcttt accctgaccg atcttctcac
    18481 ctttgtagct gtaccacgcg cctgctttct cgatcagctt ctcttttacg cccaggtcaa
    18541 ccagttcgcc gtagaagttg ataccttcgc cgtagaggat ctgaaattca gcctgtttaa
    18601 acggtgcagc gattttgttc ttcaccactt tcacgcgggt ttcgctaccc accacgtttt
    18661 cgccctcttt caccgcgccg atacgacgga tgtcgagacg aacagaggcg tagaatttca
    18721 gcgcgttacc accggtagtg gtttccgggt taccgaacat cacaccaatt ttcatacgga
    18781 tctggttgat gaagatcagc agcgtgttgg actgcttcag gttacccgcc agcttacgca
    18841 tcgcctggct catcatacgt gccgcaaggc ccatgtgaga gtcgccgatt tcgccttcga
    18901 tttccgcttt cggcgtcagt gccgccacgg agtcaacgac gataacgtct actgcaccag
    18961 aacgcgccag ggcgtcacag atttccagtg cctgctcgcc ggtgtccggc tgggagcaca
    19021 gcaggttgtc gatatcgacg cccagtttac gtgcgtagat tgggtccagc gcgtgttcag
    19081 catcgataaa cgcacaggtt ttaccttcac gctgcgctgc ggcgatcacc tgcagcgtca
    19141 gcgtggtttt accggaagat tccggtccgt agatttcgac gatacggccc atcggcagac
    19201 cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt agagatggtt tccacatcca
    19261 tggaacggtc ttcacccagg cgcatgatgg agcctttacc aaattgtttc tcaatctggc
    19321 ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat agccattttt actcctgtca
    19381 tgccgggtaa taccggatag tcaatatgtt ctgttgaagc aattatactg tatgctcata
    19441 cagtatcaag tgttttgtag aaattgttgc cacaaggtct gcaatgcata cgcagtagcc
    19501 tgacgacgca ccgcatcacg gtcgccgctg aagcattccc gccgggtaat gccttcaccg
    19561 cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct tctcttcact gccgccatcc
    19621 ggcccggcga taccactaat agacacggca taatcagcac gagccgcttt cagtgcgcct
    19681 atcaccattt ccaccacgac gggttcactc accgcgccat gctgcgccag cgtctcttcg
    19741 cgtacgccga tcatctgcgc tttggcttcg ttactgtagg tgacaaatcc gcgttcaaac
    19801 caggcggagc taccggcaat atcggtaatc actttcgcta cccaaccacc ggtacaagac
    19861 tcggcagttg ttacggttgc gccacgggct ttcagcgcct gcccaacctg ttcacttaac
    19921 tgcatcagtt cactgtcagt catcacaacc tctgtcagtc aaaaatttat gccggacaag
    19981 atagcacttt cgtcaggaag atggtgttgg cgttgcaatt ttacgaggag gattcagaaa
    20041 aaagctgatt agccagaggg aagctcacgc ccccctcttg caaatagtta ctgcacgcgc
    20101 gccagcgcca cggcttgtcc taactgccag accgccattg cgtaatgggt gctgtggtta
    20161 taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt actggtaacc ggtaccgaca
    20221 tccagacgca gcaggctggc ttgttgatgg ttgcccagcg gctgctgtgg cgttaaacct
    20281 gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc catttggcaa gcctggagcc
    20341 tgaccgtttg ccattaccgc gacctgatcg cctttcaccc agccgtgcgc tttgaaatag
    20401 ttcgccacgc taccgatcgc atcaaccgga tcccacaggt tgatatgccc gtcgccgctg
    20461 aaatctaccg catattgttt gtaagacgac ggcataaact gtccgtagcc catcgccccg
    20521 gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat cagcaggaag
    20581 gtttccagct cgccagagaa atactccgcg cggcgtgggt agttaaatga cagcgttgcc
    20641 agcgcatcga ggatgcgagt tttccccatc acgcgccccc agcgggtttc aacgccgata
    20701 atcccgacga taatttccgg cggtacgcca tacacctgcc acgcgcgatt caacgcatct
    20761 tcatactgat tccagaaaac cacaccgttc tgcacgttgt ccggcgtaat aaattttttg
    20821 cgataacgca gccatgcgcc gttcggacct gatggcggtt tcaccgatgt ggttggtgcc
    20881 tggttatcca tcagccgcag taccgaatcc agacgcttcg cctgggagag aatttcctgc
    20941 aactgctgac gatcgaaacc gtgtttgttc accattttgt cgatgaactg ctgggcattc
    21001 gggttattag cgaaatcgcc gcccatctgc atcacattgt gctgcggctc aagcaggaag
    21061 ccgccagacg gcgttccggt ggtcgtttca gtctcagtag gttttggctt gctgctacag
    21121 gcggcaagca acacaaaaag gggaagcaat gttacataac gacgcttgaa catgaggggt
    21181 ccatttaaca gattcaacca ggggcaagta tggtaaagca tcacgccccg cacaaggaag
    21241 cggtagtcac tgcccgatac ggactttaca taactcaact cattcccctc gctatccttt
    21301 tactcaaact tccaaattaa aatatttatc tttcattttt agattaaaat aacactttta
    21361 aatctttcaa tctgattaga ttaggttgcc gtttggtaat aaaacaataa atcctgaagg
    21421 agagaacaat gatagaaacc attactcatg gtgcagagtg gtttatcggg ctgttccaaa
    21481 agggcggaga ggtgtttacc gggatggtga ccggcattct tccgctgttg attagcctgc
    21541 tggttatcat gaacgcactg attaatttta tcggtcagca tcgtattgaa cgttttgctc
    21601 aacgttgcgc cggtaaccct gtttcccgtt acctgctgct accgtgcatt ggcacgtttg
    21661 tcttttgcaa tccgatgacc ttaagccagg gtcgctttat gccggaaaag tacaaaccca
    21721 gctactacgc ggcggcctct tatagctgcc actcaatgaa tggcctcttt ccccatatca
    21781 accctggcga actgtttgtt tatcttggca ttgccagcgg tctgacaacg ctgaacctgc
    21841 cacttggccc actggcgttg agctatctgc tggttggtct ggtcaccaac ttctttcgcg
    21901 gctgggtgac cgatctgacc accgccattt ttgagaaaaa gatgggcatt caacttgaac
    21961 aaaaagttca cctggcagga gcaacatcat gacgcgtatt cggatcgaaa aaggaacggg
    22021 tggctggggc ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg tctatatcac
    22081 cgccggtacc cggcctgcga ttgttgacaa actggcacaa cttactggct ggcaggctat
    22141 tgacggattt aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg taatcgactg
    22201 tggcggcaca ttacgctgcg gcatctatcc gaaacgacgt attcccacca ttaatatcca
    22261 ctcgacgggc aagtccggtc cgctggcgca gtacattgtg gaagatattt atgtctctgg
    22321 cgtaaaagaa gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct cttccgtggg
    22381 ccgtgactat gacaccagta agaaaatcac cgaacaaagc gatggtttac tggcgaaggt
    22441 gggaatgggc atggggtccg ccgttgcggt gctgtttcaa tctggtcgtg acaccatcga
    22501 cactgtatta aaaaccattc tgccgtttat ggcattcgtc tcggcgctca ttggcatcat
    22561 tatggcttct ggccttggtg actggattgc ccacggtctt gctccgctgg cgagccatcc
    22621 actgggtctg gtcatgctgg cgctcatctg ctccttccca ctgctttcac ctttcctcgg
    22681 cccaggcgca gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga ttggtctcgg
    22741 caatattccg ccgcatctgg ctttaccggc actgtttgcc atcaacgcgc aggcggcctg
    22801 cgacttcatc ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg ttcgcgtcgg
    22861 tgtcccttct gtactggtga gccgcttttt aaccggcgca ccgactgtac tgatcgcctg
    22921 gtttgtctcc ggttttatct atcaatagag gctgaaacat gaccgttatt tataagacca
    22981 ccatcacccg tatcggcgcg agtgccactg acgccctcag cgaccagatg ctcatcacct
    23041 ttcgtgaagg cgcgccagcg gacctcgaag agtattgctt cattcattgc cacggcgagt
    23101 tgaaaggtgc actccatccc ggtttgcaat ttacgctcgg gcagcatcgc tatccggtga
    23161 ccgctgttgg cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc accctgcgct
    23221 tcgatggttt aaacgaagcg gaatttccgg gcactgtcca tgtggcaggc cctgtacccg
    23281 acgatatcgc gccgggatcg gttttgaagt ttgaatctgt taaggagtaa aaaatgaatc
    23341 aggttgccgt tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc cacggtctgg
    23401 ttgccgaggg gtatcgcgtc gcggttgtcg atattcagag cgacaaagcc gcaaatgtgg
    23461 cacaagaaat taacgccgaa tatggtgaaa gtatggcgta cggttttggt gctgacgcca
    23521 ctagcgagca aagcgttctg gcgctctctc gtggggtaga tgaaatcttt ggtcgcgtgg
    23581 atttgctggt ctatagcgcc ggaatagcaa aagcagcctt tatcagcgac ttccagctcg
    23641 gcgattttga ccgttcgcta caagtgaatc tggtgggtta tttcctgtgt gcccgtgaat
    23701 tttcgcgttt gatgatccgc gacgggattc agggtcgcat tattcagatc aactcgaaat
    23761 ccggcaaagt gggcagcaaa cacaactctg gctacagcgc agcgaaattt ggtggcgtcg
    23821 ggctgactca atcactggcg ctggatctgg cggagtacgg cattacggtg cattcactga
    23881 tgctcggtaa cctgctgaaa tcgccgatgt tccagtcact gttgccacaa tacgcgacca
    23941 agctgggtat caaaccggat caagtcgagc agtattacat tgacaaagta ccgctcaaac
    24001 gcggctgcga ttatcaagat gtgctgaata tgctgctgtt ctacgccagt cctaaggcgt
    24061 cgtactgcac cggacagtcg atcaatgtca ccggcggtca ggtgatgttc tgatcaacag
    24121 cggagatcca ttaaggatct ccgtgagact atagaatgcc tgatgcgcta cgctcatcag
    24181 gcatacagga cttccgccac tacattaagg aaaagttatg gtatccgcac tcatcaccgt
    24241 cgccgttatc gcctggtgtg cgcaactggc cttaggcggc tggcaaattt ctcgttttaa
    24301 ccgtgccttc gacacactat gccagcaagg gcgggttggc gtgggtcgtt ccagcgggcg
    24361 ctttaaaccg cgggtcgtgg tcgccatcgg ctggacgatc agcagcgcat cgtcgacacc
    24421 ttgtttatga aaggactgac cgtcttcgcc cgaccgcaaa aaattcccgc aattaccggt
    24481 atgcatgcgg gtgatttaca gcccgatgtg atctttcccc atgatccact atcacagaat
    24541 gctctatcat tggcgcttaa actgaaacgt ggataatttc gttgtgaatg ttacttgctt
    24601 gcgaagttat cattttgaaa cctaaatcag gtaatcacgc ccatgaaacc tcgtcagcgt
    24661 caggccgcca ttctggagta tctgcaaaag cagggtaaat gctcggttga agaattggcg
    24721 caatactttg acaccacagg cacaaccatt cgcaaagatc tggtcattct ggaacatgcc
    24781 ggaaccgtca ttcgtactta tggcggagtg gtgttgaata aagaggaatc cgatccgcct
    24841 atcgatcata aaacactcat caacacccac aagaaagagc tgattgcaga agctgccgtt
    24901 agttttatcc atgatggcga ttcgatcatt cttgatgctg gcagtaccgt tttgcagatg
    24961 gttcccctgc tctcgcgctt taataacatc acggtgatga ccaacagcct gcatatcgtc
    25021 aatgcgctat ccgaactgga taacgaacaa actatcctga tgccaggcgg aacgtttcgt
    25081 aaaaaatcgg cctcatttca cggacagctg gcagagaatg ccttcgaaca tttcagtttc
    25141 gataaattgt ttatgggcac cgacggcatc gatctcaatg cgggcgtaac caccttcaac
    25201 gaggtttata ccgtcagtaa ggcaatgtgc aatgccgcgc gcgaagtgat tttgatggcg
    25261 gactcatcaa agtttggccg taaaagcccc aacgtagttt gcagtcttga aagcgtcgat
    25321 aagctgatta ccgacgcagg tatcgatccg gcgtttcgtc aggcgctgga agagaaaggg
    25381 atcgatgtga tcataaccgg agagagcaat gagtgaagca ctactgaacg cgggacgtca
    25441 gacgttaatg ctggagttgc aggaagcaag ccgtttaccg gaacgtctgg gcgatgattt
    25501 tgttcgcgcc gccaatatca tcctgcactg cgaaggcaaa gtggtggttt cgggaattgg
    25561 caaatcgggc cacattggta agaaaatcgc cgcaacactt gccagtaccg gcacaccggc
    25621 tttttttgtc catccggcag aagcactgca cggcgatctg gggatgattg aaagccgcga
    25681 tgtgatgctg tttatctctt actccggtgg cgcgaaggaa ctggatctga ttattccgcg
    25741 tctggaagat aaatctatcg cgctgctggc gatgaccggc aaaccgacgt caccgctggg
    25801 cctggcggca aaagcggtgc tggatatctc cgtagaacgc gaagcctgcc cgatgcacct
    25861 tgcgccgacc tccagcaccg tcaataccct gatgatgggt gacgcgctgg cgatggcggt
    25921 catgcaggcg cgcggattta atgaagaaga ttttgcccgc tcccacccag ccggggcact
    25981 gggcgctcgc ttgctgaata aagtgcatca tctgatgcgc cgtgacgatg ccatcccaca
    26041 ggtggcgtta accgccagcg tgatggatgc gatgctggaa ctcagccgca ccggtctggg
    26101 gctggtggcg gtatgtgacg ctcaacaaca ggtacaaggc gtctttaccg acggcgattt
    26161 acgtcgctgg ctggtaggcg gcggcgcact caccacgcca gttaatgaag cgatgacgac
    26221 aggcggcacc acgttacagg cgcaaagtcg cgccatcgac gccaaagaga tcctgatgaa
    26281 gcgcaaaatc actgccgcac cggtggtgga tgaaaacggc aaactcaccg gcgcaattaa
    26341 cctgcaggat ttctatcagg ccgggattat ttaatccttc attcccagac gtttcgccag
    26401 ccgatgcagg ttggcgacgt cggtttccag catccgcgcg caggcagccc agttatgatg
    26461 attttgtgcc agtgcctggc gaatagtttc acgctggaac gcttctgtcg cttcacgcag
    26521 gttttgctta acaacgggca ccgccgccgc ttctggcggc ggcaacgtca cctcaggaaa
    26581 agcaaaatgt tgcgcctcaa gaatcacttc atcgccgttg cgggtggctc tcgccagcac
    26641 taccgcccga tgaatagcat gttccagttc gcgcacgttc cccggaaaac gatagtgttg
    26701 cagtaaattt cgcgctccgg cacttaatac cacgcgggag agccccagcc gcaaacgaca
    26761 ctgttcgcag aaataccccg ccagcagaat gacatcatcg ccccgctcac gcagcggcgg
    26821 caccgaaagt ggaaacacgc tcaggcgatg aaacaagtca gcgcgaaatc gccctgccag
    26881 cacctcttcg cgtaagtcgc ggttagtcgc cgccagcacg cgcacatcga cccgcaaact
    26941 acggtcatcg ccaacgcgct gaatatcgcc atactgcaac accctcagca gcttggcctg
    27001 caatgccaac gacaactcgc cgatctcatc cagaaacaga gtgccgttat ccgccatttc
    27061 aaacttcccg ctgcggttac tgatagcgcc agtaaacgct cctttcacat gcccgaacaa
    27121 ctcactttcc gccacacttt ccggcagtgc agcacagttg agatagacca gcggattcac
    27181 cgcccgtggc gaggcttcat gaatcgcttt cgccaccagc tccttaccgg ttcccgtctc
    27241 accgctgatc aggacgttga gatcggacgc cgccacaatc tcaatctctt ttttcagttg
    27301 cgtcatgcca ggggacaagc caatcatctg cgtctgtttc accgcttcaa acggcgtggc
    27361 atcgcctggc atcatattct ggctttccag ttgttcaatc agcaacgcat tgcttaacgc
    27421 tcccgccgcc agcgcagcaa tcagccgtag ctcttcgtcg ctgaaaacat cgaactgatc
    27481 gggctgcatc ccgtcgagcg tcagtgcgcc gatcaggttt tgcccggcaa acaatggcag
    27541 accaacgcag gcgtgaacct tcagactctc ctgcccagga atcaaaccgt catagggatc
    27601 gggcaattcg ctgtctgcgg gaaagcgcac cacatccccg gcgcgggcaa tcgcttccag
    27661 ccgtggatgc ccttccagcg caaagcgtct accgagtaca ccctttgcca gaccgtcgat
    27721 ggcaagcgga ataaactgcc gcgaatcgta acgtagcaac gcagacgcat cgcactccag
    27781 cacctgacgt agcgtggtga tcaggcgctg aaaacgatcc tgatgaccaa tcccacgctg
    27841 caattcgatg gcgatattcg ccagcacatc aacggaaaaa ctcatctttg cctcactgtc
    27901 aatttgacta tagatattgt catatcgacc atttgattga tagtcatttt gactactcat
    27961 taatgagcat aattttattt atagagtaaa aacaatcaga taaaaaactg gcacgtaatc
    28021 tgcaattagc aagacatctt tttagaacac gctgaataaa ttgaggttgc tatgtctatt
    28081 gtggtgaaaa ataacattca ttgggttggt caacgtgact gggaagtgcg tgattttcac
    28141 ggtacggaat ataaaacgct gcgcggcagc agttacaaca gctacctcat ccgcgaagaa
    28201 aaaaacgtgc tgatcgacac cgtcgaccat aaattcagcc gcgaatttgt gcagaacctg
    28261 cgtaatgaaa tcgatctggc ggatatcgat tacatcgtga ttaaccatgc agaagaggac
    28321 cacgctgggg cgctgaccga actgatggcg caaattcccg atacgccgat ttactgtact
    28381 gccaacgcta tcgactcgat aaatggtcat caccatcatc cggagtggaa ttttaatgtg
    28441 gtgaaaactg gtgacacgct ggatatcggc aacggcaaac agctcatttt tgtcgaaacg
    28501 ccaatgctgc actggccgga cagcatgatg acttacctga caggcgacgc ggtgctgttc
    28561 agtaacgatg ctttcggtca gcactactgc gacgagcatc tgttcaacga tgaagtggat
    28621 cagacggagc ttttcgagca gtgccagcgt tactacgcca atatcctgac gccgttcagc
    28681 cgcctggtaa caccgaaaat taccgagatc ctgggcttta acttaccagt cgatatgata
    28741 gcgacctctc acggcgtggt atggcgcgat aacccgacgc aaattgtcga gctgtacctg
    28801 aaatgggcgg ctgattatca ggaagacaga atcaccattt tctacgacac catgtcgaat
    28861 aacacccgca tgatggctga cgctatcgcc caggggattg cggaaaccga cccacgcgtg
    28921 gcggtgaaaa ttttcaacgt cgcccgaagc gataaaaacg aaatcctgac caatgtcttc
    28981 cgctcaaaag gcgtgctggt cggcacttcg acgatgaata acgtgatgat gccgaaaatc
    29041 gccgggctgg tggaggagat gactggttta cgcttccgta acaaacgcgc cagtgctttc
    29101 ggctctcacg gctggagcgg cggtgcggtg gatcgtcttt ccacgcgcct gcaggatgcg
    29161 ggtttcgaaa tgtcgcttag cctgaaagcg aaatggcgac cagaccagga cgctctggag
    29221 ttatgccgtg aacacggtcg cgaaatcgcc cgtcagtggg cgctcgcgcc gctgccgcag
    29281 agcacggtga ataaggtagt caaagaagaa acctctgcca ccacgacggc tgacctcggc
    29341 ccacggatgc agtgcagggt ctgccagtgg atttacgatc cggcaaaagg cgagccaatg
    29401 caggacgttg cgccaggaac gccgtggagt gaagtcccgg ataacttcct ctgcccggaa
    29461 tgctccctcg gcaaagacgt ctttgaagaa ctggcatcgg aggcaaaatg agtaacggca
    29521 ttgtgatcat cggttcgggc ttcgccgccc gccaactggt gaaaaatatt cgcaaacagg
    29581 acgccactat tccattaacc ctgattgccg ccgacagcat ggatgagtac aacaaacctg
    29641 acctcagcca tgttatcagt caggggcaac gtgccgatga ccttacccgc cagacggcgg
    29701 gtgaatttgc cgagcagttt aatctgcacc tgtttccaca aacctgggtg acggatatcg
    29761 atgccgaagc ccgtgtggtg aaaagccaga ataatcagtg gcaatacgac aagctagtac
    29821 tggcaaccgg tgccagtgcc tttgtcccgc ctgtgcctgg gcgtgagtta atgctgacgt
    29881 taaatagtca gcaagagtat cgcgcctgtg aaacgcaact gcgggatgcc cgacgcgtgt
    29941 tgattgttgg cggtggtttg attggtagcg aactggcgat ggatttttgt cgtgcaggca
    30001 aagcggtcac gctaatcgac aacgctgcca gtattctggc gtcgttaatg ccaccggaag
    30061 taagcagccg cttgcagcat cggttgacgg agatgggcgt tcatctgctg ttgaaatctc
    30121 agttacaggg gctggaaaaa acggattctg gcattctggc aacgctggac caccagcgta
    30181 gtatcgaagt ggatgcggta attgccgcca ccggactgcg cccggaaaca gccctggcac
    30241 gacgcgccgg gctgacgatt aatcgcggcg tttgcgtcga tagttatctg caaaccagta
    30301 atgacgatat ttatgcgctg ggcgattacg cggaaatcaa cggtcaggta ctgccattcc
    30361 tgcagccgat tcaacttagc gcgatggtgc tggcaaaaaa tcttctcggc aataacacgc
    30421 cgctgaaact accgacgatg ctggtgaaaa tcaaaacgcc ggaattaccg ctgcatctgg
    30481 caggcgaaac ccagcgtcag gatttacgct ggcaaattaa taccgaacgc tagggaatgg
    30541 tggctcgcgg tgttgacgat gctgaccagc ttcgcgcctt tgtggtcagt gaggatcgga
    30601 tgaaagaggc atttggattg ttgaaaacat tgccgatgta ggtgggctac tgtgcctaaa
    30661 atgtcggatg cgacgctagc gcgtcttatc cgacctacgg ggacgcatgt gtaggccgga
    30721 taaggcgttt acgccgcatc cggcaatggt gtccaaatgc aacacgtttt atccgttctg
    30781 gacttcaccc gctaaccaac gcgccgcagc aataaccccc tgccccagag acaaaccgcc
    30841 atcacccgcc ggtaaactct gtggaaagag caatgtgaaa tcagcgagat aatgcgccag
    30901 acgtgcacgc agcaaacggt tatgaataac cccgccgcta aataccagcg tagtgatacc
    30961 acgcatcgtg gcctgctcac gcatcaacgc ggcaaaaccc tgcgccagcg catcatgaaa
    31021 cgcccacgcg cgttgattaa cctgtgcctg ccagttcagc cactgctgcc agaaagcggc
    31081 gagatccagt tgattgtcca ccagcggcat cgtcaccgga tgcgtcactc cgtggcatga
    31141 ggctgcgagc gcctccagag cacaagccgc ttcaccttca taacttaacg tggctggcgc
    31201 acagcccagt gccgccgcca ctgcatcgaa caaccgccca cacgatgacg ccagcggcgc
    31261 gttaattcca cgctcaatgg cccgcgccag cacgctccag ttttgctgtt gcacacttgc
    31321 tgtttcaggg taattctgcc actccggcac aaagcgcagg cactgcgcca gcaggtttcg
    31381 ccacggctgc ttcgctgcca aatcgccacc cggaagcgcc actgcaggca agccgcccag
    31441 gtgctcacat tcgcgatagt tcacccgcag gcactcgccg ccccacaaag cgccgttctc
    31501 ccccataccg ataccgtcga gcgtcaaagc aatgacatca ccgccatcca gcggccactg
    31561 atgctctgcc agacacgccg ctgcatgggc atgatgatgc agtaccgttt gcgtcggcag
    31621 attcatttca cgcgcccact ggctggagac atagcccgga tgcgcgtcat gcacaacgta
    31681 tttcggggta aaatcgtaga tgttttgcat caggcgtaac gcttcgcgcc actgcatctg
    31741 gatgccatcg tcacttaaat cgcccagatg ctgactcaac accgcttgtt caccgcgcac
    31801 caggcagaag gtatttttca gatccgctcc gagacacagc acaggcggaa catttttaaa
    31861 gcccggaggc aaagccagcg catccggcac atacccccgc gaacggcgca gcatttcgcc
    31921 gctttcgcgc accaccgaat catccatccg ctgcacgatg tcgcggttat gtatcaagaa
    31981 tccgtcggca atgccctgca aatccgccag cgcctgttcg ttgctgatag ctggtggttt
    32041 accgctcagg ttgccggagg tcatcaccag cgggcattgc agttcctgta acagcaaatg
    32101 ctggagcggg ttcgcaggca acattacccc gacttcgtta aggtcagggg cgatatcatc
    32161 acaaagctca ggaacgtatt ttttatccac cagcacaatc ggcgcggcgg gcgtggtaag
    32221 caactggcgc gcagcgtctg gtaaaccgtc agccactggc aacatgaccg ccagcggttt
    32281 cgccgggcga tgtttgcgcg cccgaagtgt cgccaccgcg ttactgttac gtgcatcgca
    32341 ggcaagatga aatccgccaa tccctttgat ggcgacaatg ttgcccattt ttaactgtgc
    32401 gatagccgcc tgtaatgccg cctcttgttc tgcatgttca ccatgactta cccattcaag
    32461 atgcgggcca cactccgggc aggccaccgg ctgggcgtgg aagcgacgat cgagcgggtc
    32521 gcggtactct ttgtcacaag ccggacacaa cggaaacgcc gccatcacgg taaacgggcg
    32581 gtcgtaaggc atggcgcgaa taatggtgaa acgcgggccg cagtgggtac agttgataaa
    32641 cggataacga taacgccgtt cgcctggggt attcatttcg gcaaggcaag cagggcaagt
    32701 agcggcatcc gggacaattt gcgtattcat ggcacctccc gcgctctggc ggatggtgaa
    32761 ctcagtgggc agttgtgacc agatgtacgg ctcacgctcg acgctatcaa tacgcgccag
    32821 cggcgggcag tgctgatgca attgaacaag aaacgtttcc gggtcttccc gcagccggac
    32881 ttctacgcca tcgccgtcat tacagacatc gccgtgaaga tttaattgct gtgccagctg
    32941 ccagacaaac ggacgaaaac cgacgccctg cactttgcca cgaatacgca gttggacacc
    33001 gcaagatgtg ttttttgcca ttgagttatt cccgccatca tgaattgcgt aacccgccca
    33061 gccggacacg acagcgtcgc atccggcagt cacaggtcgg cgataccgcc cgctccgtat
    33121 tctacgaata tttccgggaa ttcctttgat gccagaacag ttctgtaaga tttttagaac
    33181 atcagcgccg tacggcggcg tttttctgca ctcagttgtt caagtttatt acgatcgaca
    33241 caaatcagcg catgagtcgg gcaagccgcc atacacgccg ggccgtcttc acgatggttg
    33301 cacaggtcac atttattggc ttcggctttg tcagcccgta cattcagccc cgcgccgctg
    33361 ttgcgaatca ccggacgtac caccacttcc atcgcgccat acgggcaagc cacaacgcag
    33421 gttttgcaac caatgcaacg ttcctgcatc acatgaacaa accctttatc acggctgata
    33481 gcaccattcg ggcagacgtt agcgcacggt gcatcttcac actgacggca aactgtcgcc
    33541 gtggaaatgt tcacaccttt aatgacatgg atacgcggta aaaatgtttc cggggtcagc
    33601 gatgcacagt cctgattttc ctgatgagaa accacgcacg ccacttcaca ggtacggcaa
    33661 ccaatacatt tactcgcgtc agcaatgatg aaacggttca tcaaattctc cagcaatgac
    33721 agttaatgcg ccgatacatt cacaaatcat gccagttttt aattcactgt tatttaagga
    33781 aattaatttc tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga tgacatgtga
    33841 tgacaatgtt tatcgcgaaa gagcaatgag tgagtcgcgg tggatcagtt ttccgctgaa
    33901 ggttttcggc ggtgagaaat ccccgccatc gagcataaaa atcagccgtc caataatttc
    33961 ctgaatcatc tcagtcaccg gaatttttac gctggagagc gccggaacga tatagggggc
    34021 aattgcgata tcatcgaatc cgataactga cacctgctct ggcaccgcta cgccgcgctc
    34081 gtgcaacgct ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa
    34141 tttagcccca cgttcgagca acgtttctac cccttcggcc ccgctggcgg gcgtccattt
    34201 accgttagcg ataagttttt cattgagcgc aataccatgc tgcgccagcg cgtctttata
    34261 cccagcaaga cgttcaatgc tggtggggga atccatcgag ccggtaagga aagcaatctc
    34321 ctgatgtccg gcgtttatca actctgccac ggcattaaag ctggtctgtt tatgatcgca
    34381 ccagacgcta tggctgctgt ttttgcgcag gcggcgatta agcaccatta tcggctgact
    34441 gtgcgcgtca atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt aaatcataat
    34501 ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg cgctcttctt cggcgctgtg
    34561 tttaccatct gccaatagca actgccgccc tttctcttcc gccattcgcg cggcatgaaa
    34621 gagtaattca ctaaaataaa tgccgtggta aagcgtgttg gtcactacca gccccagcgt
    34681 ctgagtactc ttcgccgaca gattgcgcgc cagcaagttt gggcggtaac cgctctcttc
    34741 taccgcctga aacacgcgat ctttagtctc ctggctgacg tagccattac ctgaaagcac
    34801 gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat
    34861 cattttcatc cctttacacg caatcaacgc agtgtactgc accgtttgcc gattgtcctt
    34921 gcgcaatcgg cgggaaaaat attcaggtga ccggtttcac aaatataaaa aatgaataat
    34981 taactctctt gcttatttag tgacaactat tcatgatttt gtgaaaccgg tttcctaatt
    35041 ccgtttcagc atcggcattt ttccgttacg tcgactgata acaactacat ctaccctact
    35101 gataacagga taaaatccga tggccaaaaa ttatgcggcg ctggcacgct cggagatagc
    35161 ggcactgggc ggcgttgata acatctcggc ggtcacgcac tgtatgacgc gtttgcgctt
    35221 tgttatcaaa gatgaccagc ttatcgacag cccgacgtta aaaaccatcc ccggcgtgct
    35281 cggcgtggta cgtagtgaca accagtgtca ggtgattatc ggcaataccg tttcacaagc
    35341 ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag cccgcacagc ccgtgggtaa
    35401 acccaaactc acgccgcgtc gcattggtgc ggggatcctc gatgcgctga tcggcaccat
    35461 gtcaccgctg atcccggcga ttatcggcgg atcgatggtc aaactgctgg cgatgatcct
    35521 cgagatgagc ggcgtgctga caaaaggatc gccgacctta accattctga atgtgattgg
    35581 tgacggtgct ttcttcttcc tgccgctgat ggtcgcggca tctgccgcca tcaaatttaa
    35641 aaccaatatg tcgctggcga ttgccatcgc gggtgtgctg gtacatccga gttttattga
    35701 actgatggcg aaagcggccc agggtgaaca tgtcgaattt gccctgattc cggtcaccgc
    35761 ggtgaaatac acctacacag tgatcccggc gctggtgatg acctggtgcc tgtcatatat
    35821 cgaacgctgg gtggatcgca ttacaccggc ggtgaccaaa aacttcctca agccaatgct
    35881 gattgtgttg attgccgcac cgctggcaat cctgctgatt ggcccgattg gtatctggat
    35941 cggtagcgcc atttcggcgc tggtttacag cattcatggt tatctgggct ggctttcagt
    36001 cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc gcgtctttac
    36061 gccaaccatc attcagacca ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga
    36121 gatcggcgct aacctgtcgc tgggcggttc atcactggcg gtggcgtgga aaacgaaaaa
    36181 cccggaactg cgccagacgg cgctggctgc ggcggcatca gccattatgg cggggatttc
    36241 cgaaccggcg ttatacggcg tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat
    36301 cagcggtttt atttgcggcg cggttgccgg tatggcgggg cttgccagcc actcaatggc
    36361 agcgccgggg ctatttacca gcgtgcaatt cttcgatccg gcgaatccaa tgagcatcgt
    36421 ctgggtgttc gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca cactgttgct
    36481 cggctttgag gatattcctg ttgaggaagc ggctgcccag gcgcgaaagc atcagagcgt
    36541 acaaccgacc gtcgccaaag aagtaagtct taattgagga tgaaaatgtc agtatttcca
    36601 gaaggttttt tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc gttccgtgaa
    36661 ggcggaaaag ggctgaccac cgtcgatatg atcccacacg gcgagcatcg aatggcggtg
    36721 aaactggggc tggaaaaacg ttttcagttg cgcgatgacg agttttatcc cagccatgag
    36781 gcgacggatt tttatcatcg ttataaagaa gatatcgccc tgatggcaga gatgggattc
    36841 aaggttttcc gtacctcaat tgcctggagc cgtctctttc cgcagggcga tgaaatcacg
    36901 cccaatcagc agggcattgc tttttatcgt tctgtctttg aagagtgtaa aaagtacggt
    36961 atcgaaccgc tggtcacgtt gtgccacttc gatgtgccga tgcatctggt caccgaatat
    37021 ggctcctggc gtaaccgcaa gctggtggag tttttcagcc gctacgccag aacctgcttt
    37081 gaagcatttg atggtctggt gaaatactgg ctaaccttca atgaaatcaa cattatgttg
    37141 catagcccgt tctccggcgc gggtctggtg tttgaagaag gtgaaaatca ggatcaggtg
    37201 aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc tagccgccaa aatcgcccat
    37261 gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg gcggcaactt ctacccttac
    37321 agctgcaaac cggaagatgt ctgggcggcg ctggagaaag atcgggaaaa cctgtttttt
    37381 atcgatgtgc aggcgcgcgg cgcgtatccg gcttactctg cccgcgtatt tcgcgaaaaa
    37441 ggggtaacca tagataaagc gctgggcgat gatgagatcc tgaaaaacac cgtcgatttt
    37501 gtctctttca gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc caacaacagc
    37561 agtgcggcga acgtggtgaa atcgctgcgt aacccgtatc tgcaagtgag cgactggggc
    37621 tggggaattg atccactcgg tctgcgtatc accatgaata tgatgtacga tcgttatcag
    37681 aagccgctgt ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc tgccaatggc
    37741 gagattaacg atgactatcg catcagctat ttacgcgaac atatccgcgc aatgggcgaa
    37801 gcgattgcag atggcattcc gctgatgggc tacaccacat ggggctgtat tgatttagtt
    37861 tccgcctcta cgggtgaaat gagcaaacgc tacggttttg tctacgtaga ccgtgacgac
    37921 gcaggcaacg gcacgctgac gcgcacacgt aagaaatcgt tctggtggta taaaaaagtg
    37981 attgccagta atggggaaga tttagagtaa gtaacagtgc cggatgcggc gtgaacgcct
    38041 tatccggcct acatgtcgca cgaaccagga aggcccgata agcgcagcgc atcgggcaat
    38101 ttagcatttg gcatcagagc tggttcaccc catcaagaac atccctgtcc tgattcctta
    38161 atgaaaaact actcttcttc caccgctaac tgcgcgaagc cgccattccc ttcccagcct
    38221 tccagtcgtt gataaacggt ttctaccgca tctttaatcg gctgggtcat cgggtagtaa
    38281 aagccgacga tatccggctg tatgccgagg aaaatcactt cgccaatatc ttctttcaac
    38341 tggtcgataa gatagttcag aggcatgtta tgggtagtca tcataaacat ctcggcgata
    38401 tcatccgggt cgatgatgcg gatctcgccg gggtttagcc ccatatccgt ggcgtcgaca
    38461 atcagtaacc gcgtagggcg cagttcacgg atggcgacga tgtcgttttc cggtgcacta
    38521 ccgccgtcaa tcaccaccca gttacctttc ggcgcggcgg cgcacttttc cgccagcagc
    38581 ggacctgcgc catcatcgcc catcatgcta ttgccaacac agagtaaaac gtcagtcacg
    38641 caatctcctc accatcaaat agatggcgtt ttcctgatga atatcatgca gcatacttaa
    38701 cagcattgta ctccattcct gctgctgcga agtttgcgct gcccgggctt tatcgaatgc
    38761 gttggccagc atcggcacat ggttaatgtc gatgacgatc tcaccgtact tcggtacgcc
    38821 ttccattttg cggcgggctt cactgcccgc ctccagagtg gcaatccatg ccagatattc
    38881 atcccacggg caggtgagcg ccgcttccag gcaatcgata accccaaggt ggtgaccaat
    38941 cgccaggctg taatagacca cctgctgcgc ctcggcgggc gtggcatcgt tctcatcaat
    39001 aaatttacgg ctcagttgac tgaacaccac cttttcactc atcggatacg cacctcttct
    39061 acaacatgat tcagatggct gacaatctcg ttcagacgcg gatcgttttc cgcttccagc
    39121 cagcgtgcaa cctgttcttc gccctgccct aactgtgtca ggaagtcatc ggcaatctga
    39181 cgaccgtaac gataacccgc caggcggcgt gcttcgcgat ccactttcac gcgcagcggc
    39241 tgcaccatat cgccatgcag gatctccgcc ggttgttcat ccagttcacc cggcccacgg
    39301 gcgtgaattt tctgctccag caggccgagc gccattgcaa agccgtacag cgtggcggca
    39361 ggcgttggcg ggcagccagg gatataaaca tccacaggga caattttatc cgtaccgccc
    39421 cacacgcagt agagatcgtg gaagatcccg ccgctgttac cgcaggcacc gtaggagata
    39481 caaattttcg ggtccggcgc ggactgccac gcacgcagcg caggggatcg cattgcacgg
    39541 gtgaccgcgc cggtaaacag taaaatatcc gcatgacgcg gtgaaggaac gactttaatg
    39601 ccgaagcgtt ctgcatcaaa cagcggcgat aaagtggcga aaatttcgat ttcgcagccg
    39661 ttgcagccgc cgcagtccac gcggtaaaca taggcagaac gtttgatttt tttcagtaac
    39721 gacgccttca tgctggcgat ggattcatcc accgtcatcg ggaccggaat gccgttggcg
    39781 tcacgggggc ctaataaatt gctcatcaga tggcctcttt catatggcga gtcagttcaa
    39841 tacggtcgga cggcaccagg catttctggc gcttacattc cgggcaagtc tcaaagcttt
    39901 cgcggtggtt ttccgcgcgg ctgtcgccgt tgtgcttaag cagcgcaatg gcgtagtcga
    39961 tctctttctg gacggcgaaa ggacgattgc agacgcgaca gttgcacagc gcgaagcgag
    40021 actgttgcag gaagtcttct ttcttccaca ccgccagttc gtactcttgc gacagcttaa
    40081 tcgccgccgt cgggcaaact tcttcgcagc gtccacagaa gatgcagcgc ccgagattaa
    40141 actcccaggc cagctctcct gtggcgaggt cagtttcaac cgttaaggcg tttgacgggc
    40201 aggcattgac gcaggccgcg cagccgatgc actgctgcgg gttctgctct ggcttaccac
    40261 ggaagttttt atcaaccgca atcggctcca gcggataaga cgaggtcgcc gtaccggttt
    40321 tgataacttt tttgataaag gtaaacatgg cgattcctta tttcagcggc gagtttttac
    40381 gctcaatgct gtagcgctcg agttctttgt acggcaccac tttgctcttc ttcttacgta
    40441 catcgaccac agtcatgcgg tcggtacagg agtagcaagg gtccaggcta ccgataatca
    40501 gcggcgcatc ggaaacagtg ttgccacgca gcatgtagcg cagggtcggc cagttcgcgt
    40561 aggtcgcggc acggcagcgc cagcggtaca gcttctggtt gtcgccagtc atgctccagt
    40621 ggatatcatc accgcgcggc gcttcggcaa agcccagcgc gaaacggtgc ggaatgtagg
    40681 taaagccttc caccatcagc gggccacccg gcaggttatc cagaccgtag tcgatcatgt
    40741 tcagcgcggt atagacttcg ttgatacgca ctttcagacg cgaaataacg tcgcagccct
    40801 gctcgctgtg gacttccatt ggcagcaggc cataaccaac aaacgggtga tcagcgcggg
    40861 tatcgcgagc gtgtccgctg gcgcggacca tcgggccaac gttactgaaa tcgcgggcaa
    40921 tttccgggtc cagacgacca atgccgacgg tgcgctgttc catgttcggt gtgctcagca
    40981 gcacatccac cagctcctgc acttcacgac gcatctgttg cgccagctgg cgggtctgga
    41041 tcatgtcatc tttcaacaga tcgcgacgaa tcccgccgat caggttcagg ccgtaggttt
    41101 tacgcgcacc ggtgaggatc tcggccattt tcatggaggt ttcacgcacg cggaagaatt
    41161 gcataaagcc ggagtcgaag ccggtaaagt ggcaggcgag gccgaggttg agcagatgcg
    41221 agtgcaggcg ttccacctcc agcagaatgg cgcggatcat ctgcgcacgt tctggcacct
    41281 gaatacccat cgcgttttcc accgacgtgg tgtaggcggt gctgtgggcg aagccgcaga
    41341 tcccgcacac acggtcagag aggaaggtca cttcgttata acccatgcgg gtttccgcca
    41401 gtttttccat accgcgatgg acatagaaca agcggtagtc tgcgtcgata atgttttcgc
    41461 catcgacgaa cagacggaag tggcccggtt cgtcagaagt gacgtgcagc ggaccaatcg
    41521 gcacgacgtt gtttttcttg tcgcccagtt cgttgatgaa ctcgtaggtt tcagcatcgg
    41581 tggtcggtgc cggacgctga cgataatcca tgctgtcttt acgcagcgga taaagttcat
    41641 ccggccagtc atccggcagc accagacggc gttcatccgg cagaccaacc ggaatcaaac
    41701 cgtacatatc gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc ggcgtcacgg
    41761 acggatactc cggtttgttg gcatcaactt cgacgcgaac cgttacccag cacttagtcc
    41821 ccttctccat cgacagcacg taataaacgg cgtaatgacc attcagtttg cgttcgtcgt
    41881 taccaaaaag caccgacagc cagcccccct gcttgtagta gagaaactcc accacttccg
    41941 gcagatagtt cacctttacg gtgacagtca gctgctcttt ggtctgccag gcgtggtcca
    42001 gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct aatttttctt
    42061 cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg ctaaaaatgc
    42121 aaagccaaac ccagcccagg taatgcgcga agtaatatca agacgcagac gggccatgct
    42181 gttttcgaac agcgcgataa ccaggacgcc gaccaccagt tttacgatcg caatcaccag
    42241 cgccagcagc agtccaccgg cggtgaaggt ttccatttgt ccccacggaa taaacacccc
    42301 gacgaacatc tgcaacacca ccagctgttt caggctgata ccccatttca tgacgccaaa
    42361 gccgctgccg ctatactcag agagcggacc ttcctgcaac tcctgctcgg cttccgccag
    42421 gtcgaacggc agtttgccca tttcgataaa agtggcgaac gcacaggcgc agagcgccag
    42481 caccagcggg atgctttggc tcagcggcca gtgataaacg gtgtcggtaa tgttgctgat
    42541 gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcaaca tcggttcgac
    42601 cagcacgcca agcatcgctt cacggctcgc accgatagcg gtaaacgggc taccggtatc
    42661 cagaccggaa atggcaaaga agaaacgcgc gatggcgaag agatacagta aggtgatcaa
    42721 atcgcccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag tagcgatagt
    42781 cagcatgacg cccaccatca catacggcgt caggcggaac acccagccag aggcatccgg
    42841 gccaacgctc tggcggccca gcagtttgat aatgtcgcga tactcctgca acacgcccgg
    42901 cccgcgacgg ttatgcagac gggcgcgcgc cacgcgggta ataccggaga gcagcggcgc
    42961 aacggcaaat aacaccagcg cctgaattaa cggatataaa acactcattc tcaggctcct
    43021 cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg gcggaacagc
    43081 aacgcactac cttcgcactg ccagcccggc accagcgaca ccggattcag ccatttgcgc
    43141 agtttcagca ctggcgcaaa cgcctgttta accggcatgg caaaaccgtg agcggtaatc
    43201 accattgatt tttcatggtc gtagccgcac acccaggccg caccgcggga acgtgacggc
    43261 aggcggtcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca ggcaatcagc
    43321 agcaatgtga tcattggttg agaaacggtg gtgttagcag gctccagagg cagaggtacg
    43381 gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac gcagcaaatc
    43441 gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt ttcggcttct
    43501 ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca catcaccgcc
    43561 agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg ggcaacaaac
    43621 gcgccactat tgctcagttt gaaaaaggat tgatagataa cccattcccc ggcaaacccg
    43681 ttcagcggcg gcagcgcagc cattgccatc aacccgacta acatggcgat ggagataacc
    43741 ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt acggaaccag
    43801 acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag cagatggtac
    43861 agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat acccgttacg
    43921 ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta agccagcagg
    43981 cgctggatgt tgtgctccat cagcgcatac agaccaccga caaacgcggt gatcatgccg
    44041 agcaccagca gcacgatccc ccaccacagc ggtgcattac cgcccagcag tgacaggatt
    44101 aaaatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc agctggcgca
    44161 gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc ggcgagcagg
    44221 ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg ttgcatacgc
    44281 atatccagca ggcgcagatc cagcgtgcca taacgctgcc acagcagcca gcaagcaatc
    44341 gccagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc tttgctgttg
    44401 ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa catgccgagg
    44461 ttgctggcga tgacggcgca gacggcagca gccatcaaca tattgatctg caagccgttg
    44521 cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa cagaccgcac
    44581 agaccgagcg taatcagcca aatcgcgtta agcggggaga tttgcacatc gtagcttacc
    44641 agcgacagcg caccgctcac gccaaccgcg ccagtcagca cagtgaagcc cgcggctgcc
    44701 gtatacaggc taccaaccgc gccgccaatt ccggctatcc agccacttag cgtcttttga
    44761 aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc acaccacgcc gctattgatc
    44821 agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag gtgagatcgc
    44881 caaagtccgt attaaaagtc agctcacgct tacgtttgct gacgcgggcg atatcggtgt
    44941 tatccaccag atgcagggct ttggtcgggc acatccgcac gcaggccgga ccttgttcat
    45001 caaagctaca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc cagtcaagca
    45061 gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta ttggcatttg
    45121 caggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg caggcgatgc
    45181 cgcacagctt gcagcttacg cacaggcttt cattcaactg cacggcccca tcaacgcggg
    45241 tgatgacgtt aaccgggcag accaccgcgc agggtgcatc ttcacagtgg tgacagagct
    45301 gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat tgcaggccgt
    45361 gctggcgatg cgtctctgaa caggctgcct cacaagtgtg gcagccgata cagagcgtgg
    45421 agtcagcaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat ttttgacgaa
    45481 aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc aaacaggcgt
    45541 aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc ctttaccagt
    45601 tggcttaaat tcaccggcac attgttttca atggcggtgt ataagctgtc gtaaacgcca
    45661 gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt gaccttgcca
    45721 tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc ttcgccctct
    45781 tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag ggtgacgtaa
    45841 atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca gctcatggcg
    45901 tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca gtagatgtgc
    45961 cactggtcct gtaggcgacg atgccgctgt gcgatgtaat cggctttctc gcttatttcc
    46021 caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc attttctgtg
    46081 ccaattttta atttattgtt attaaaacga tttttaagct aaagatgaat ttcgtcgccg
    46141 tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat caacactggc
    46201 acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga aataaccctc
    46261 tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc aaaacgcgta
    46321 actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc tcttgccttt
    46381 tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca cctcgaagaa
    46441 caagaggtcg aatgctggtg tgaaacatgc caacagtatg tgacgctatt gacccagcgc
    46501 gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga cgacggttta
    46561 cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg cggttgcggt
    46621 gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt tcgtagcgcg
    46681 ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc gcctgaattt
    46741 acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc gggcactcac
    46801 gcaccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct ggacaaaaac
    46861 aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact ggtgctcaac
    46921 ctggtttcca gcccaggttc cggtaaaacc accctgctga cggaaaccct aatgcgcctg
    46981 aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa cgatgccgca
    47041 cgcattcgcg ctaccggcac accagcgatt caggtgaaca caggtaaagg ctgccatctt
    47101 gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa cggtattctg
    47161 tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg tgaaaaacac
    47221 aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata tccgcatatg
    47281 tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta tctcaacttt
    47341 gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga aatcatcctt
    47401 atttccgcca ccagcggtga agggatggac cagtggctga actggctgga gacacagcga
    47461 tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggca aaagtcgacg
    47521 tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat gaaaacggtc
    47581 agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc gtaattactg
    47641 aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt gagccggatg
    47701 tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat atcgcgcgcc
    47761 ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc tctcttacac
    47821 cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg ctatttttaa
    47881 attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc cgggttgccc
    47941 ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca gccatccgga
    48001 agtcatcttc tgtacctttg gcgatgccat gcgcgtacca gggaaacagg gatcgctgtt
    48061 gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg atgcgttgaa
    48121 actggcgcag gagaatccaa cccgcaaagt ggtgttcttc ggcttaggtt ttgaaaccac
    48181 catgccgacc accgccatca ctctgcaaca ggcgaaagcg cgtgatgtgc agaattttta
    48241 cttcttctgc cagcacatta cgcttatccc gacgctgcgc agtttgctgg aacagccgga
    48301 taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg gcactgatgc
    48361 ctataatttt atcgccagcg attttcatcg tccgctggtg gtcgctggtt tcgaacccct
    48421 tgatctactg caaggcgtgg tgatgctggt ggagcagaaa atagcggccc acagcaaggt
    48481 agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc aacaggcgat
    48541 tgccgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg tgattgaatc
    48601 ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac atttccgccc
    48661 ggcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat taacgggcaa
    48721 atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa ccgcatttgg
    48781 tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc gtcagcagga
    48841 gagtgaagcg tgaataatat ccaactcgcc cacggcagcg gtggccaggc gatgcagcaa
    48901 ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga gcaggaagac
    48961 caggcacgtc ttgatctggc gcagctggta gcagaaggcg atcgtctggc gttctccacc
    49021 gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa gctggcgatt
    49081 tgcggcaccg cgaatgacgt tgcggtcagt ggcgctattc cgtgctatct ctcctgtggc
    49141 tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac cagcatggca
    49201 gaaaccgccc gcacggcagg cattgccatc gttactggcg atactaaagt ggtgcagcgc
    49261 ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc ggcgaatatt
    49321 cactggggcg cacagacgct aaccgcaggc gatgtattgc tggttagcgg tacactcggc
    49381 gaccacgggg cgactatcct taacctgcgt gaacaactgg ggctggatgg tgaactggtc
    49441 agcgactgcg cggtgctgac gccgcttatt cagacgctgc gtgacattcc cggcgtgaaa
    49501 gcgctgcgtg atgccacccg tggtggtgta aacgcggtgg ttcatgagtt cgcggcagcc
    49561 tgcggttgtg gtattgaaat ttctgaatca gcactgccgg ttaaacctgc tgtgcgcggc
    49621 gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa actagtaata
    49681 gctgttgaac gcaacgcagc agagcaagtg ctggcagcgt tacattccca tccactgggg
    49741 aaagacgcgg cgctaattgg tgaagtggtg gaacgtaaag gtgttcgtct tgccggtctg
    49801 tatggtgtga aacgaaccct cgatttacca cacgccgaac cgcttccgcg tatatgctaa
    49861 taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg cggtgctttc
    49921 ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag ggttgttcga
    49981 catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg ctctttcgca
    50041 actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc aagcgcagac
    50101 tcaacgtgcg tcttattacg catcgcgtga aaaagacacc cccattaaat atgaagacga
    50161 aactgttctg gcacacggtc cggtacgccg cattttgtcg cgccctgata cgctgcattg
    50221 cagttacgaa gaattttgtg aaacctggcc gcagctggtc gctggtgggc tataccccta
    50281 atttggtcac tattgcctga tgccactggc ggcggaaggg catatttttg gtggctgtga
    50341 atttattcgt tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc tgcaaacatt
    50401 tacgcagatc gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtca acaatgtcga
    50461 ctatgagttg ttatgccggg agcgcgataa cttccgcatc ctggttgcca ttaccaacgc
    50521 ggtgctttcc cgcctggata tggacgaact ggtcagcgaa gtcgccaaag aaatccatta
    50581 ctatttcgac attgacgata tcagtatcgt cttacgcagc caccgtaaaa acaaactcaa
    50641 catctactcc actcactatc ttgataaaca acatcccgcc cacgaacaga gcgaagtcga
    50701 tgaagccgga accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc tgatcaatct
    50761 ccacgagcgg gacgatttag ccccctatga acgcatgttg ttcgacacct ggggcaacca
    50821 gattcaaacc ttgtgcctgt taccgctgat gtctggcgac accatgctgg gcgtgctgaa
    50881 actggcgcaa tgcgaagaga aagtgtttac cactaccaat ctgaatttgc tgcgccagat
    50941 tgccgaacgt gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa tccatcgtct
    51001 gaaagaacgg ctggttgatg aaaacctcgc cctgaccgag cagctcaaca atgttgatag
    51061 tgaatttggc gagattattg gccgcagcga agccatgtac agcgtgctta aacaagttga
    51121 aatggtggcg caaagtgaca gtaccgtgct gatcctcggt gaaactggca cgggtaaaga
    51181 gctgattgcc cgcgcgatcc ataatctcag tgggcgtaat aatcgccgca tggtcaaaat
    51241 gaactgcgcg gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc atgagcgtgg
    51301 ggcttttacc ggtgccagcg cccagcgcat tggtcgtttt gaactggcgg ataaaagctc
    51361 cctgttcctc gacgaagtgg gcgatatgcc actggagtta cagccgaagt tgctgcgtgt
    51421 attgcaggaa caggagtttg aacgtctcgg cagcaacaaa atcattcaga cggacgtacg
    51481 tttaattgcc gcgactaacc gcgatctgaa aaaaatggtc gccgaccgag agttccgtag
    51541 cgatctctat taccgcctga acgtattccc gattcacctg ccgccactac gcgagcgtcc
    51601 ggaagatatt ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc gtctggggcg
    51661 caatatcgac agcattccag ccgagacgct gcgcaccttg agcaacatgg agtggccggg
    51721 taacgtacgc gaactggaaa acgtcattga gcgcgcagta ttgctaacac gcggcaacgt
    51781 gctgcagctg tcattgccag atattgcttt accggagcct gaaacgccgc ctgccgcaac
    51841 ggttgtcgct caggagggcg aagatgaata tcagttgatt gttcgcgtgc tgaaagaaac
    51901 caacggcgtg gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga aacgcacgac
    51961 cctgctgtca cggatgaagc ggctgggaat tgataaatcg gcattgattt aactgcaaat
    52021 tgccggacag atctgcctgt ccggcatact attcacgagg ttttttcgga cgatattttt
    52081 ccggcagttc tggcaccgga cgcttgtcat cgatgagatg acgcacggtt aagatcggat
    52141 gacgccacag cattctcggc ccggcccaac gcataatctg tttcatctct tcacgctttg
    52201 caggctggta acagtgcacc ggacactgct tacaggctgg tttctcttcg ccgaacacac
    52261 atttatccag ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc ggctccgctg
    52321 acgcctgcgg gcatttcgct tgataaagat cgatcatttt tttaatcgtc agtttttcac
    52381 gagagatacg cttgccggac atgctgcctc catactctta aggtgtattt atattacaac
    52441 ttaatttgaa aggggactat ggctctgaag gaggaggttt attcaaccga cgcaattttg
    52501 cgtacttgct tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc cggaaccagc
    52561 atcaagaact cgccgttcgc ttcttcccct gaaatgatta actccggtat catgtgcgcc
    52621 ttatgtgatt acaacgaaaa taaaaaccat cacaccccat ttaatatcag ggaaccggac
    52681 ataaccccat gagtgcaata gaaaatttcg acgcccatac gcccatgatg cagcagtatc
    52741 tcaggctgaa agcccagcat cccgagatcc tgctgtttta ccggatgggt gatttttatg
    52801 aactgtttta tgacgacgca aaacgcgcgt cgcaactgct ggatatttca ctgaccaaac
    52861 gcggtgcttc ggcgggagag ccgatcccga tggcggggat tccctaccat gcggtggaaa
    52921 actatctcgc caaactggtg aatcagggag agtccgttgc catctgcgaa caaattggcg
    52981 atccggcgac cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt acgccaggca
    53041 ccatcagcga tgaagccctg ttacaggagc gtcaggacaa cctgctggcg gctatctggc
    53101 aggacagcaa aggtttcggc tacgcgacgc tggatatcag ctccgggcgt tttcgcctga
    53161 gcgaaccggc cgaccgcgaa acgatggcgg cagagctgca acgcactaat ccggcggaac
    53221 tgctgtatgc agaagatttc gccgagatgt cgctgattga aggccgtcgc ggcctgcgcc
    53281 gtcgcccgct gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat ctgcaatttg
    53341 gcacccgcga tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt tgtgctgccg
    53401 gttgtctgtt gcagtatgcg aaagataccc aacgcacgac cctgccgcat attcgttcta
    53461 tcaccatgga acgtgagcag gacagcatca ttatggatgc cgcgacacgt cgtaacctgg
    53521 aaattactca gaacctggcg ggcggtgcgg aaaatacgct ggcttctgtg ctcgactgca
    53581 ccgtcacgcc gatgggtagt cgtatgctga aacgctggct gcatatgcca gtgcgcgata
    53641 cccgcgtgtt gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc accgccgggc
    53701 tacagccggt actgcgtcag gtcggcgacc ttgaacgtat tctggcacgt ctggctttac
    53761 gaactgctcg cccacgcgat ctggcccgta tgcgccacgc tttccagcaa ctgccggagc
    53821 tgcgtgcaca gttagaaact gtcgatagtg caccggtaca ggcgctacgt gagaagatgg
    53881 gcgagtttgc cgagctgcgc gatctgctgg agcgagcaat catcgacaca ccgccggtgc
    53941 tggtacgcga cggtggtgtt atcgcatcgg gctataacga agagctggat gagtggcgcg
    54001 cgctggctga cggcgcgacc gattatctgg agcgtctgga agtccgcgag cgtgaacgta
    54061 ccggcctgga cacgctgaaa gttggcttta atgcggtgca cggctactac attcaaatca
    54121 gccgtgggca aagccatctg gcacccatca actacatgcg tcgccagacg ctgaaaaacg
    54181 ccgagcgcta catcattcca gagctaaaag agtacgaaga taaagttctc acctcaaaag
    54241 gcaaagcact ggcactggaa aaacagcttt atgaagagct gttcgacctg ctgttgccgc
    54301 atctggaagc gttgcaacag agcgcgagcg cgctggcgga actcgacgtg ctggttaacc
    54361 tggcggaacg ggcctatacc ctgaactaca cctgcccgac cttcattgat aaaccgggca
    54421 ttcgcattac cgaaggtcgc catccggtag ttgaacaagt actgaatgag ccatttatcg
    54481 ctaacccgct gaatctgtcg ccgcagcgcc gtatgttgat tattaccggc ccgaacatgg
    54541 gcggtaaaag tacctatatg cgccagaccg cactgattgc gctgatggcc tacattggca
    54601 gctatgtacc ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt acccgcgtag
    54661 gcgcggcaga tgacctggct tccgggcgct caacctttat ggtggagatg actgaaaccg
    54721 ccaatatttt acataacgcc accgaataca gtctggtgtt aatggatgag atcgggcgtg
    54781 gaacgtccac ctacgatggt ctgtcgctgg cgtgggcgtg cgcagaaaat ctggcgaata
    54841 agattaaggc attgacgctg tttgccaccc actatttcga gctgacccag ctaccggaga
    54901 aaatggaaga cgtcgccaac gtgcatctcg atgcgctgga gcacggagac accattgcct
    54961 ttatgcatag cgtgcaggat ggcgcggcga gcaaaagcta cggcctggcg gttgcagctc
    55021 tggcaggcgt gccaaaagag gtgattaagc gcgcacggca aaaactgcgt gagctggaaa
    55081 gcatttcgcc gaacgccgcc gctacgcaag tggatggtac gcaaatgtct ttgctgtcag
    55141 taccggaaga aacctcgcca gcggtcgaag ctctggaaaa cctggacccg gattcactga
    55201 ctccgcgtca ggcgctggaa tggatttatc gcttgaagag cctggtgtaa taacaattcc
    55261 cgatagtctt ttgctatcgg gaatattaac gacaactgac gaataaaata aaaaccccct
    55321 gtataatagg aaagcttatt ttacagggta aaaccatgcc atctacacgc tatcaaaaaa
    55381 ttaatgccca tcactatcgc catatatggg tcgttggtga tattcatggt gaatatcaat
    55441 tattacaatc ccgcttacat caactctctt tttgccctga aaccgactta cttatttctg
    55501 tcggcgataa tattgatcgt gggccggaga gtcttgacgt cctgcgcctg ctaaaccagc
    55561 cctggtttat ctccgttaaa ggcaaccacg aagcaatgga gctggatgcg ttcgcgaccg
    55621 gcgatggcaa tatgtggctt gccagcggtg gtgactggtt tttcgattta aatgattcag
    55681 agcaacaaga ggcaatagat ctgttgctga aattccatca ccttccacat attattgaaa
    55741 tcactaacga cataataaaa tatgtcatcg cacatgcaga ttatccgggt gatgaatatc
    55801 tctttggcaa agaaatagcg gagagcgaat tactctggcc tgtttatcgt gtgcagaaat
    55861 cgcttaatgg cgagttacaa caaataaacg gcgctgatta ttttatattt ggacatatga
    55921 tgtttgataa cattcagacg ttcgctaacc agatttatat tgataccgga tcgccgaaaa
    55981 gtgggcggct gtcattttat aaaataaggt agtctcatgc ttcttctgtg aagcatgagt
    56041 aaccagatgt tattgcaggc cattattcat ttttcgctac cagcaaagag agatcctgct
    56101 tcgccagcgc gcgactggca ctctccggca aaccgtcgtc tgtaataatc tgatcaaact
    56161 cgcttaatgg taacgccagc catgtcgcca cctgaccata tttcgtcgca tcacagacca
    56221 aaactcgctg gcggctggca ctagcaatcg cccgtttcac cgtgacttta tcttccgctg
    56281 gcgtagaaat cccccgcaca ctccatgacg atgcagaaat aaaagcctga tcaatcatca
    56341 ggctgcgcag catggtcgca gcggcttccc cgacacagga acggttttcc cgacacactg
    56401 caccgccagt gtgaataatt gtgcaattac tgttgtcgag caagtagttc gcaataacga
    56461 aatcgtttgt gaccacagtc agtgactcca tgtgaatcag atgctgtgct atcgctaacg
    56521 tggtcgttcc cgcatccaga tagatacaac ttcccggctg aacaagactt gccgccagct
    56581 tgccaatagc cgctttttgc gtcattgcta gcgcagtttt tacctgatga gaaggttcat
    56641 gcgccacgcg tcccggagac tggacgcctc cggacaccag cacaacggct ccctgctgct
    56701 ccagtttttg taaatcccga cgaatggtca tatgtgatac attcattctg tccgttagtt
    56761 cagcaatact gacaatgcct ttttcagcta ccatctcaag gatgatttgg cgacgctcta
    56821 cgggtatcaa cttttgctcc ttcctttgtc ctgctgacat tctacgctat ttgcctgcga
    56881 aacgtgcgcg gcgcaactaa cgcttagttc acataaaata acacacaatg ttaatttatg
    56941 tgaataggct caccatatcg ttatcttcca gcgcttatat tcacaatatc aaacaaaata
    57001 tcacttaaat taacaaggag agcagatgaa aacgggatct gagtttcatg tcggtatcgt
    57061 tggcttaggg tcaatgggaa tgggagcagc actgtcatgt gtccgcgtag gtctttctac
    57121 ctggggcgca gacctgaaca gcaatgcctg cgctacgttg aaagaggcag gtgcttgcgg
    57181 ggtttctgat aacgccgcga cgtttgccga aaaactggac gctctgctgg tgctggtggt
    57241 caatgcggcc caggttaaac aggtgctgtt tggtgaaaca ggcgttgcac aacatctgaa
    57301 acccggtacg gcagtaatgg tttcttccac tatcgctagt gctgatgcgc aagaaattgc
    57361 taccgctctg gctggattcg atctggaaat gctggatgcg ccagtttctg gtgatgcagt
    57421 aaaagccgct aacggtgaaa tgactgtcat ggcctccggt agcgatattg cctttgaacg
    57481 actggcaccc gtgctggaag ccgttgccgg aaaagtttat cgcataggtg cagaaccggg
    57541 actaggttcg accgtaaaaa ttattcacca gttgttagcg ggcgtacata ttgctgccgg
    57601 agccgaagcg atggcacttg cagcccgtgc ggggatcccg ctggatgtga tgtatgacgt
    57661 cgtgaccaat gccgccggaa attcctggat gttcgaaaac cggatgcgtc atgtggtgga
    57721 tggcgattac accccgcatt cagccgtcga tatttttgtt aaggatcttg gtctggttgc
    57781 cgatacagcc aaagccctgc acttcccgct gccattggcc tcaacagcat tgaatatgtt
    57841 caccagcgcc agtaacgcag gttacgggaa agaagacgat agcgcagtta tcaagatttt
    57901 ctctggcatc actctaccgg gagcgaaatc atgatcaaga ttggcgttat cgccgatgat
    57961 tttaccggcg cgacggatat cgccagtttt ctggtggaaa acggtctacc aacggtacaa
    58021 attaacggtg ttccaacagg taaaatgccg gaagcaatcg acgcactggt gatcagcctg
    58081 aaaacgcgct cctgtccggt ggttgaagcc acacagcaat cgctggcggc tctgagctgg
    58141 ttgcaacagc aaggttgcaa acagatctat ttcaaatact gctctacttt cgacagtacg
    58201 gcgaaaggta atatcggccc ggttaccgat gcattaatgg atgctctcga cacgccgttt
    58261 acggtcttct ctccggccct gtcggtcaac ggacgtacgg tttatcaggg gtatttgttc
    58321 gtaatgaatc aactgctggc cgaatccggg atgcgccatc acccggtaaa tcccatgacc
    58381 gacagctatc ttccccgtct ggttgaagcg caatccacag ggcgctgcgg cgtcgtttcg
    58441 gcacatgttt tcgaacaagg tgtggatgcc gttcgtcaag agctggctcg cttacagcaa
    58501 gagggctacc gctacgcggt gcttgatgcg ctgaccgaac accatctgga aattcaggga
    58561 gaagccttgc gcgatgcccc actggtaacg ggcggttctg gtctggcgat tggcctggcc
    58621 cggcagtggg cgcaagaaaa cggtaaccag gctcgcgaag cagggcatcc gctcgctggg
    58681 cgcggcgtag tgctctccgg ttcatgctct caaatgacca accgccaggt ggcacattac
    58741 cgtcaaattg caccagcccg tgaagttgat gtggcacgct gcctctcaac cgaaactctg
    58801 gccgcttatg cacacgaact ggcagagtgg gttctgggcc aggaaagtgt acttgctcca
    58861 ctggtttttg ccaccgccag cactgacgca ttggcagcaa ttcaacagca atacggtgca
    58921 caaaaagcca gtcaggcagt agaaactctg ttttctcaac tagcggcgcg gttagcagcg
    58981 gaaggcgtga cacgctttat tgtcgcaggc ggtgagacct ccggcgtagt cacacagagc
    59041 ctgggcataa aagggtttca tattggccca accatttccc ccggcgtgcc gtgggtaaac
    59101 gcactggata agcctgtctc actcgccctt aaatctggca acttcggtga tgaagccttt
    59161 ttttcacgag cccaaagaga gtttttatca tgagcgattt cgcaaaagta gagcagtctt
    59221 tgcgagagga gatgacgcgg attgccagtt cattctttca gcgcggctat gcaaccggtt
    59281 cggctggcaa tctgtcgctg cttttacctg acgggaattt actggcgaca ccgacaggtt
    59341 catgcctggg caatctcgat ccgcagcggc tttccaaagt cgccgcggat ggcgaatggt
    59401 taagtggtga caaaccctcg aaagaggtgc tctttcatct ggcgctgtat cgcaacaatc
    59461 cgcgctgtaa agcggtggtg catttgcaca gcacatggtc gacggtgctt tcctgcctgc
    59521 aagggctgga cagcagcaac gttattcgtc cgttcacacc atacgtggtg atgcggatgg
    59581 gaaatgtccc gctggtgccc tattaccgac cgggcgataa acgcatcgca caggatctgg
    59641 cggaactggc agcagacaat caggcttttt tactggcaaa tcatggccca gtggtttgcg
    59701 gtgaaagcct gcaagaagcc gccaacaata tggaagagct ggaggaaacg gcaaagctga
    59761 tttttattct cggtgaccgc ccgatccgtt atctgaccgc aggtgaaatt gcggaattaa
    59821 ggagttaatg caatgcctcg ttttgcagct aatttatcca tgatgttcac cgaagtgcct
    59881 tttattgaac gcttcgccgc agcgcgaaaa gccgggttcg atgctgtgga atttctgttt
    59941 ccctatgact actccaccct gcaaatccaa aagcaactgg agcaaaacca tctgacactg
    60001 cgctgttcaa taccgctccc ggagatatta atgcggggga atggggatta tccgcccttc
    60061 ccggacgtga gcacgaagca cacgcagaca tcgacctggc actggaatat gcattggcgc
    60121 tcaattgtga acaagtccat gtgatggcag gcgtcgtgcc cgctggcgaa gatgctgaac
    60181 ggtaccgggc agtatttatc gataatctcc gctacgctgc cgaccgcttt gcgccacacg
    60241 gcaagcgaat attagttgaa gcactcagcc ccgacgttaa gcctcattat ctcttctcca
    60301 gccagtatca ggcactggct attgttgaag aggttgcgcg agataacgtg tttattcaac
    60361 tagatacttt tcatgcgcaa aaagtagatg gcaacctgac gcatttaatc cgcgactacg
    60421 ctgggaaata tgcgcacgta caaattgccg gactacctga tcgacatgac cggacgatgg
    60481 agaaatcaac tatccgtggc tgttccgcct gttcgatgag gtgggatatc agggctggat
    60541 cggttgtgaa tataaacctc gtggcctcac cgaagaaggg cttggctggt ttgacgcctg
    60601 gcgctaattc gttaaatccc taattacaac gtacccatac atccccccat ctacggatgg
    60661 ggtaggttaa ctataacttt cagacagggt ttcccatgtc cacaattaca ttgttatgca
    60721 ttgcgttagc tggcgtaatc atgctgttgc tgctggtcat caaggcaaag gtacaaccat
    60781 tcgttgctct gctcctcgtc agcctgttag tcgcacttgc ggcgggtata ccggcgggcg
    60841 aagtgggtaa agtgatgatc gccgggatgg gcggtgtgct tggttccgtc actattatta
    60901 ttggtctggg cgctatgctg gggcgtatga tcgaacactc tggtggtgca gagtcactgg
    60961 ctaattattt cagtcgcaag ttaggtgaca aacgaactat cgctgcgctg actctggcag
    61021 cgttcttcct cggtattccc gtcttctttg atgtcagctt tattattctt gcgccaatca
    61081 tttacggttt tgccaaggtt gccaaaatat cgccactcaa atttggcctg cctgtcgctg
    61141 gaatcatgct aactgttcat gtggcagtac cgccgcatcc aggccctgtc gccacagcgg
    61201 ggttactcca cgcagacatc ggctggctaa ccatcatcgg tattgcgatt tctattcccg
    61261 tagggattgt tggctacttt gcagcgaaaa taatcaataa gcgccaatat gcgatgtcag
    61321 tagaagtact ggaacagatg caactggctc cggccagtga ggaaggcgcg acaaaattaa
    61381 gcgataaaat aaatccaccg ggcgtcgcgc tggtcacctc gctaattgtt attcctatcg
    61441 cgattatcat gacgggtacg gtttccgcaa cactgatgcc gccttcgcat cccctgcttg
    61501 gtacgctaca gctgatcggc tcaccaatgg tagctctaat gattgcgctg gtgctggcat
    61561 tctggttatt ggctttacgt cgcggctgga gcttacaaca caccagcgac attatgggct
    61621 cagcccttcc cactgcggcg gtagtgattt tggttactgg tgctggaggg gtatttggca
    61681 aagtgctggt ggaatcgggc gtcggcaaag cccttgccaa tatgctacaa atgattgacc
    61741 tgcctctgtt accagccgca tttattattt cactggctct gcgtgcatcg caggggtcag
    61801 caaccgtagc aatcctgaca accggcgggt tactctcaga agcggtgatg ggattgaatc
    61861 cgattcagtg cgtattggtg acgctggcag cctgctttgg tgggcttggc gcgtcacata
    61921 ttaatgactc agggttctgg attgtgacca aatatctggg gttgtcggta gcagacggtc
    61981 tgaaaacctg gacagtgtta acgaccattc tcggttttac cggattctta attacctggt
    62041 gcgtatgggc ggtaatttga ccttaataaa aaggtccgat gggcatcgga ccttttattg
    62101 tgcacagaaa aggccagcct cgcttgagac tggcctttct gacagatgct tacttactcg
    62161 cggaacagcg cttcgatatt cagcccctgc gtttgcagga tttcacgcaa acggcgcagg
    62221 ccttcaacct gaatctggcg aacacgttca cgggtgaggc caatttcacg acctacatct
    62281 tccagtgttg ccgcttcgta ccccagcaaa ccgaatcgac gtgccagcac ttcacgctgt
    62341 ttggcgttca gctcgaacag ccatttgacg atgctctgct tcatatcgtc atcttgcgtg
    62401 gtatcttccg gaccgttctc tttttcatcg gccaggatgt ccagcaacgc tttttcggaa
    62461 tcaccaccca gcggggtgtc taccgaggta atgcgctcgt taagacgaag catacggctg
    62521 acgtcatcaa ctggcttatc cagttgctct gcgatctctt ccgcacttgg ttcatggtcc
    62581 agcttatggg acaactcacg tgcggttcgc aggtaaacgt tcagctcctt tacgatgtga
    62641 atcggcaaac gaatagtacg ggtttggttc ataatcgccc gttcaatcgt ctggcgaatc
    62701 caccaggttg cgtatgttga gaagcggaaa ccacgttccg ggtcaaactt ctctaccgcg
    62761 cggatcagcc ccaggttgcc ctcttcgata aggtccagca acgccagacc acgattgcca
    62821 taacggcggg caatttttac caccagacgc aagttactct cgatcatccg gcggcgagag
    62881 gcgacatctc cacgcagtgc gcgacgcgca aaataaactt cttcttcggc cgttaacagt
    62941 ggtgaataac caatctcacc aaggtaaagc tgagtcgcgt ccaacactcg ctgtgtggct
    63001 ccctgcgata acagttcctc ttcggccaaa tcgttatcac tgggttcctc ttctactaag
    63061 gccttttcgt caaaaacctc aactccgttc tcatcaaatt ccgcatcttc atttaaatca
    63121 tgaactttca gcgtattctg actcataagg tggctcctac ccgtgatccc ttgacggaac
    63181 attcaagcaa aagcctggtt ccgccgattt atcgctgcgg caaataacgc agcgggttta
    63241 cggatttccc cttgtaacga atttcaaaat gcaagcgtgt tgaactggtt ccggtgctac
    63301 ccatggttgc tattttttgc cccgccttca cttcttgttg ttcccggacc agcattgtgt
    63361 cgttatgggc gtaggcactc aggtaatcat cattatgttt gatgataatc agattaccgt
    63421 agccgcgcag cgcgttaccg gcataaacaa cgcggccatc tgcggtcgcg ataattgcct
    63481 gtcctttgct gcctgcgata tcaatcccct tgttgccccc ctcagaagcg ccaaaggttt
    63541 cgatcacttt gccctcagtc ggccagcgcc aggtggagat aggcgtactg gttgatgtac
    63601 tgctgacagt tggctcggtt gtgcttgctg ttggtaccgt tacaggcgct gtgaccgtgg
    63661 tcgcagctgg cttgttgttc ggcaacattt tgttagcact ctgttcaccc gaagattcag
    63721 aatacgtaat tgtcggttgc gacgcaacag caacggtgga attttgtgca ggcttgatca
    63781 caactccttg ctctgctgcg tcggcctggg taatggcatt tccgccagtg attggcgtac
    63841 cggaagcatt acccacctgc aaggtctgac caacgttcag cgcgtatggt gcctgaatat
    63901 tgttgcgctg agcaaggtca cggaaatcgt tgccagtaat ccaggcgata tagaaaagtg
    63961 tgtcgccttt tttcacggta taggtactgc cgctataact gcctttcgga atgttcccat
    64021 actgacggtt atagacgatg cgtccgtttt ccatctgtac cggctgctga gctactggct
    64081 gcactggctg gatttgcggt tgctgggctg tagacgtcgt ccccattttc ggcggcggcg
    64141 taatcaacat accagaattg gtatttgcag gcgcattgcc attaacggag ctgaccggtg
    64201 ccggtggatt tgaagtgtca gaacagcctg ccagccatag cgaaaccagt gacaaagccg
    64261 caatgcggcg aacggtgaat tttgggcttc ccgcgctcat ttatccccca ggaaaaattg
    64321 gttaataacc agtgacataa ttaccgtgca aggcacccta ctgaacactg gaaaagatgt
    64381 tcacgatacg ctgacctgcg gcaaaataac caggaaaaat ccaggtattt cctcacgttt
    64441 taagccagct cccccttcac taaagggaca aagcgcacgg cctccacggt atcgataata
    64501 aattcgcctc cccgacgacg cacccgtttc aaatactggt gctcctcccc tacgggtaag
    64561 acgagaatcc cgccttcgtc cagctgcgtc attagcgcag ttggaatttc cggcggtgcc
    64621 gccgtaacaa tgatagcgtc aaacggcgcg cgtgcctgcc aaccttgcca tccatcgcca
    64681 tgacgggttg aaacattatg taaatcaaga tttttcaggc ggcgacgcgc ctgccactgc
    64741 aaacctttaa tccgttcgac cgagcaaaca tgctggacaa gatgcgccag gattgccgtt
    64801 tgatatcccg aaccggtgcc aatttccagc acccgcgact gcggcgtcag ctcgagtaat
    64861 tcggtcattc gcgccaccat atacggctgc gaaattgtct gcccctgacc tatcggcaag
    64921 gcgatattgt cccaggcttt ttgttcaaac gcttcatcaa cgaatttttc acgcggcacg
    64981 gcagcaagtg cattcagcac ctgctcatcc tgaatacctt gcgcacgtaa ttgatccaga
    65041 agtgcttgta cgcgtctgct taccattgcg tgccaactcc cacgctgttt aaccagtctg
    65101 aaaccacatc ttgcgcgcta tgcgcagtta aatccacatg cagcggcgtg atggagacat
    65161 agccctcatc taccgcagca aaatcggtcc ccggaccggc atcacattta ccgcccggcg
    65221 ggccaatcca gtacagcgta ttgccgcgcg gatcttgctg cgggatcacc tgatctgccg
    65281 gatgtcgtgt accgcagcgc gtcacgcgaa tacctttgat ttgatccaag ggtaaatccg
    65341 gaacgttaat attaagaata cgtccggtgc gcagcggctc tttacacagt gcgcgcaaaa
    65401 ttgaacaggt taccgccgcg gcagtgtcgt aatgtttatg cccgtcaagc gagacggcca
    65461 gcgccggaaa acctaaatga cggccttcca tcgcggcggc taccgtaccg gaataaataa
    65521 cgtcatcccc cagattcggc ccggcgttaa tgccggacac aacaatgtcc gggcgcggac
    65581 gcatcagagc attcacgcca agatagacgc aatcggtcgg ggttcccatt tgcacagcaa
    65641 tatcaccatt ttcaaaggta aacgtgcgca gggaggattc cagtgtcaga gaatttgaag
    65701 cgccgctgcg gttacgatcg ggggcgacca cctgaacgtc agcaaactca cgcaaggctt
    65761 tcgccagcgt ttgtataccg ggtgcatgta ccccgtcatc attactcagc aatatgcgca
    65821 taatcacctg ttgtgttgat aagttccctg acaacgctgg ttgcaaaact acccgccgga
    65881 agccagaaac ggatctctac ggtgacgtca tcccaccaat tccagcttaa ttgttgcgga
    65941 tacagcagca tcgctctgcg cgcggcttca actttttcgc gcaccagtaa agcttgtaat
    66001 tcagtttctg cggcgacagc tgcttgttcg aatgccagcg ctttacgctg agttccccat
    66061 tcgccactgc ccggcaatgc ggcggttatc atcagctctt tatcgttgac gcgatgctgt
    66121 aattccgcca gttcttcggt ggttgctaca aaccagctac cacgtccggc taattgtagc
    66181 gcatcgccgt caacaacttg attaacgtct gcttttttga ggcgctcagc aacaatctga
    66241 ttaaacaacg cactgcgggc tgccgacaac caaaaactcc gtttattgcg atcgcgcacc
    66301 ggagtattgg tttgcgccca gcgcagcgcg ccctgcaagt tgctaccgcc aatcccaaaa
    66361 cgttgggcag cgaagtagtt cggtacacct tttacgcaaa tatcgatcag acgttgttca
    66421 acgtcatcgc gattgctcac ttcgcgtaga accagggtaa aggcgttacc tttcagcgcg
    66481 cctaaacgca gcttgcgctt gtgccgcgca tactccagca cctggcagcc ttccagttga
    66541 aaggcgctca gatcgggcat ttccttgcct ggcacgcgag cgcataacca ctgttccgta
    66601 acagcatgtt tgtctttttg cccagcgaag ctgacttcac gggcatgaat tttcaggaat
    66661 ttcgccagtg catccgccac aaaacgggta ttgcagccgt ttttgaggat tctaaccaga
    66721 atatgctcac cttcaccatc aggctcaaag cccaaatctt ccaccaccac aaagtcttcc
    66781 ggattggctt tcagcagccc ggtgccttgc ggtttaccgt ggaggtaagt gagattatca
    66841 aactcaatca ttttgttgcc ttaatgagta gcgccaccgc ttcacaggca atcccttccc
    66901 cacgtccggt aaatccaagt ttttccgtag tagtggcttt cacgttaaca tcatccatat
    66961 ggcagccgag atcttcggca ataaacacgc gcatttgtgg aatgtgcggc aacatcttcg
    67021 gtgcctgagc gatgatagtg acatcgacgt tgcccagggt ataacccttc gcctgaatac
    67081 gacgccaggc ttcgcgtagc agctcgcggc tgtcggcacc tttaaatgcc ggatcggtat
    67141 ccgggaacag cttgccgata tcccccagcg ccgccgcgcc aagcaatgca tcggtcaacg
    67201 catggagcgc cacgtcgcca tcagaatgcg ccagcaatcc tttttcgtaa ggaatgcgta
    67261 cgccaccaat gataattggg ccttcaccgc caaaggcatg tacgtcaaaa ccgtgtccaa
    67321 ttcgcattat gtattctcct gatggatggt tcgggtgagg taaaactcgg ccagtgccaa
    67381 atcttccggg cgcgtgactt taatgttatc cgcacggcct tcgaccaact gaggatggaa
    67441 tccgcaatat tccagcgccg aggcttcgtc ggtaatagtc gcgccttcat ttagagcgcg
    67501 cgtcagacag tcatgtaaca gctcacgagg gaaaaattgc ggcgtcagcg cgtgccataa
    67561 gccgttgcga tcaacggtat gagcaatggc atttttgccc ggttcggcac gtttcatcgt
    67621 atcgcgcact ggtgcggcta ggatccctcc cgtgcggctg gtttcgctca acgccaacaa
    67681 tcgcgcgagg tcatcctgat gcagacaagg acgagcggcg tcatgcacca atacccactg
    67741 cgcgtcgcca gcggctttca aacctgccag cacggaatcg gcacgctcat caccgccatc
    67801 tacaacggtg atttgcggat gattcgccag aggaagttgt gcaaaacggc tatcgccagg
    67861 acttatggca atgacgacac gtttcacccg gggatgcgcc agcagcgcat gcaccgagtg
    67921 ttcaagaatg gtttgattac cgattgagag atattgctta ggacattccg tttgcattcg
    67981 acggccaaat ccggccgccg gaaccacggc gcaaacatcc aaatgagtgg ttgccatgtt
    68041 aattcccggg ctgatttatc gattgttttg ccccgcagac tgtgcgcgct tcgacgcgtc
    68101 aggcaccaga cgataaaaag tttcgcccgg cctggtcatg ctgagttcat tacgcgcacg
    68161 ctcttcgagc gcctcctggc cgccattgag atcgtcaatt tcggcaaaaa gttgatcgtt
    68221 tcgcgcttta agtttcgcgt ttgtagcttg ctgtgccgcc acatcatcat tgacgcgggt
    68281 atagtcatgt ataccgttct taccgaacca cagcgaatac tgtagccaga ccagaatagc
    68341 cagcaacagc agcgttagtt tacccatcct gccccctgaa aaacggcatc atcatcccat
    68401 gcatccgaag acgactctac atcctctgtt ggggataccg cgacaacgcg ggcaaatgta
    68461 ccacatttgt ccattgttac gtatacctag ggcgtgcaga acataatctc attattagtt
    68521 acggtttgaa ttatgaacag aggagacaag aaagtacaaa ttagcccagt agccacataa
    68581 acagtgcgcc aaacataatg cctactgtca ccagggtgaa aacaatactg tagcgtagct
    68641 ttccgtccat caatgaatgc agcgcaatcc ccaccactac cgcgacaggc atcagtgcca
    68701 gaaagaaagg ccaggtgtag ataaagaaga acagcgtgtt agagccataa atcaacatcg
    68761 gcatcgccag cgcaaataac caggagataa aaccgaccac ggcaccaggc agtgaccatg
    68821 tggtttcttc atcctcagta aggctgtcgt tatttgttag tgtaatgtta tggctattac
    68881 gcatatttga tcctgttact ttgacgaacc gggcatggaa acccggtggt gtctcaggat
    68941 ctgataatat cgttctgtct caacagatct aataattgct gtaccaaatt tgttactaat
    69001 tgttcaccat tgagatgaat ttctgccgat tcaggcgctt cgtaaacgga atctattccc
    69061 gtaaagttgc gcagttcacc ggcacgcgct ttcttatata agcctttggg atcgcgggct
    69121 tcgcaaatcg ccagcggcgt atcgacaaac acttcgataa agcgcccttc tcctacgcgt
    69181 tcgcgaacca tctggcgttc ggcgcggtgt ggcgagataa atgcggtcag caccaccagt
    69241 ccggcttcaa ccatcaaatt cgccacttca ccgacgcgac ggatattctc tttacgatcg
    69301 gcatcgctaa aaccgagatc gctgcataat ccgtggcgaa cattgtcgcc atccagcaga
    69361 tacgtactga cgccgagttt atgtaacgcc tcctccagcg ccccggcgac cgttgattta
    69421 ccggacccag agaggccggt aaaccacagc actacaccac gatgaccgtg gtgtagctcg
    69481 cgttgttgca cagtgaccgg atggctatgc cagacgacgt tttcgtcatg cagcgccatt
    69541 atttatcccc cagcaaatcg cgcgcgcccc agtgcggaaa gtggcgacga accagggcat
    69601 tcaattccag ttcgaatgca ctgaattcag atgacgcaac ggcggcctcg tttttcggct
    69661 catgcaccat accggcacca acagtcacgt tgctcaggcg atcgataaaa atcagcccac
    69721 ccgtcaccgg attttgttga taacgatcta acaccagcgg ctcgtcaaaa gtgagatcca
    69781 cgaggccgat cccattcagt ggcaggtttt caacttcacg ctgggtaagg ttattaatat
    69841 caaccggata gcgaatgcca tcaacacgcg cgcgcgtctt cttaccggca attttgatgt
    69901 cgtaactctg gcccggggaa agcggctgtt ccgccatcca taccacatcc accgacgcgc
    69961 tcggcaccgc cggtaacgct tcgtctgccg ccagcagcag atcgccacgg ctgatgtcga
    70021 tctcatccgt cagcaccagg gtgatcgctt ctccggcaaa ggcttcttcg cgatcaccat
    70081 caaaagtcac gatccgcgcg acgtttgatt ccacaccaga gggcagcgct ttgacacgtt
    70141 gcccgacttc cacgcgaccg gatgccagcg ttccggcgta gccacgaaaa tcgagattcg
    70201 ggcgattaac gtactgcacc gggaagcgca ttggctgggc atccaccact cgctggatct
    70261 ccacggtttc cagcacttcg agcagtgtcg gaccgctgta ccacgacata ctttcacttt
    70321 gcgatgccac gttgtcgcct tccagtgcag agagcggcac aaagcggata tccagattac
    70381 ccggcagctg cccggcaaag gtcaaataat cttcacgaat atgggtgaac gtctcttcac
    70441 tgtaatccac cagatccatt ttgttgatcg ccacgaccag atgtttgatc cccaacagtg
    70501 tggagataaa actgtgacga cgggtttgat cgagcacgcc tttacgggca tcgatcagta
    70561 agatcgccag ttcacatgtc gatgcgccag ttgccatatt gcgagtgtac tgctcgtgcc
    70621 ctggggtgtc ggcgataata aatttacgct tctcggtaga gaaataacgg taagccacgt
    70681 caatggtgat gccctgttcg cgctcagctt gcaggccgtc caccagcagt gccagatcca
    70741 gcttttcgcc ctgggtgccg tgacgcttac tgtcgttatg cagcgatgag agctgatctt
    70801 cgtagatttg gcgggtatcg tgcagcaggc gaccaatcag ggtacttttg ccgtcatcga
    70861 cgctaccaca ggtcagaaaa cgcagcaggc ttttatgttg ttgcgcaatc atccaggctt
    70921 cgacgccgcc ttcattggcg atttgttgtg caagtgcggt gttcatctta aaaatacccc
    70981 tgacgttttt tcagctccat agaccccgcc tggtcgcggt caatcacgcg gccctgacgt
    71041 tcactggtgg tagaaaccag catctcttcg atgatctccg gcagtgtttg tgcatttgac
    71101 tccaccgcac cggtcagcgg ccagcagccc agcgtacgga aacgcaccat ccgtttttta
    71161 atcacttcgc ccggttgcag gtcgatacgg ttgtcatcaa tcatcatcaa cataccgtcg
    71221 cgttccagaa ccggacgttc cgcagcgaga tacagtggca caatgtcgat attttccagc
    71281 cagatgtatt gccagatatc ctgctcggtc cagttagaga gcgggaagac gcggatgctt
    71341 tcacctttgt taatttgccc gttgtagttg tgccacagct ccgggcgctg atttttcgga
    71401 tcccagcgat ggaagcggtc acggaaagag tagattcgct ctttagcgcg ggatttctct
    71461 tcatcacggc gcgcaccacc gaaggcggca tcaaaaccgt atttgttcag cgcctgtttc
    71521 aggccttcag ttttcataat atcggtatgt ttcgcgctgc cgtgtacgaa tggattaatc
    71581 cccatcgcca cgccttccgg gtttttatgc accagcagct cgcagccgta ggctttagca
    71641 gtacgatcgc ggaactcata catctcgcgg aatttccagc cggtatcgac atgcagcaac
    71701 gggaaaggca gcgtacctgg ataaaacgcc ttgcgcgcca gatgcagcat gacgctggaa
    71761 tctttaccga tagagtagag catcaccgga tttgagaatt ctgccgccac ctcgcgaata
    71821 atgtggatgc tttccgcctc cagttgccgc aggtgagtaa gtcgtttttg agccataacc
    71881 gttcctttgc aataccgcta ttttcttgcc atcagatgtt tcgactatag ggagcgtaag
    71941 agaacgaatg aaattaccaa ttagaatgag tagttcctta acggaataac gatttggcaa
    72001 agctaatatc aaaaagtgct taaggcaccg gatttcgggc gtttaggaag atttgaaatt
    72061 gttttagcgc agcggcagtt tcatactatg gcggtaaaaa aatttgcatg gtatttaagg
    72121 actcactatg ttttccgcat tgcgccaccg taccgctgcc ctggcgctcg gcgtatgctt
    72181 tattctcccc gtacacgcct cgtcacctaa acctggcgat tttgctaata ctcaggcacg
    72241 acatattgct actttctttc cgggacgcat gaccggaact cctgcagaaa tgttatctgc
    72301 cgattatatt agccaacagt ttcagcaaat gggttatcgc agtgatattc ggacatttaa
    72361 tagtcggtat atttataccg cccgcgataa tcgtaagagc tggcataacg tgacgggaag
    72421 tacggtgatt gccgctcatg aaggaaaagc gccgcagcag atcatcatta tggcgcatct
    72481 ggatacttac gccccgctga gcgatgctga cgccgatgcc aatctcggcg ggctgacgtt
    72541 acaaggaatg gatgataacg ccgcaggttt aggtgtcatg ctggaattgg cagaacgcct
    72601 gaaaaatacg cctaccgagt atggtattcg atttgtggcg accagcggcg aagaggaagg
    72661 gaaattaggc gctgagaatt tactcaagcg gatgagtgac accgaaaaga aaaatacgct
    72721 gctggtgatt aatctcgata acttaattgt tggcgataaa ttgtatttca acagcggtgt
    72781 aaaaaccact gaagcagtaa ggaaattaac gcgcgacagg gcgctggcaa ttgcgcgcag
    72841 tcacggaata gccgcaacga ccaatccggg gttgaataaa aattatccga aaggcactgg
    72901 gtgttgtaat gacgcagaaa tattcgacaa agcgggcatt gctgtacttt cggtggaagc
    72961 gactaactgg aatcttggga ataaggatgg ttatcagcaa cgcgcaaaaa cagccgcatt
    73021 ccctgcggga aatagctggc atgacgtaag actggataat caacagcata ttgataaggc
    73081 acttcctgga agaatagaac gtcgctgccg tgacgttatg cggataatgc taccgttggt
    73141 gaaggagctg gcgaaggcgt cttgatgggt ttgaaaatgg gagctgggag ttctaccgca
    73201 ggggcgggga attctaagtg atatccatta tcgcatccag tgcgcccggt ttatccccgc
    73261 tggcgcgggg aacacactaa gcatacataa ctgtttttaa acaaatttat tccgcatcaa
    73321 caatctacca actaaattaa aacatttcct tatttttaaa gaacggataa cttattgatt
    73381 atcaacagga agaaaagaaa ccaaacgtaa cccatccaaa tccaccggaa tacgtctgtt
    73441 ttctccccag gtctgaaatt caaaacccga ctcggtattg gtcgcccagg ccatcaccac
    73501 atttccgcaa ccagccagtt gggtaatttg ctgccagatc atctcccgaa tacgttttga
    73561 tgtatcacca acatacacac cggcacgcac ttccagtagc cagattgcga gccgtccacg
    73621 taagcgtggc gggacatttt ctgtaacaac cacgaccatg ctcatcagcc gcgtcctcga
    73681 tgaccgctat cgcccaacgt ttcaggttcg gggatggctg gcggtaacat atccggcgca
    73741 ggttgtggcg gttcaatttc acctgcggca aggacttcct caattaacgg tattaatttg
    73801 cccgttaact tagtgctacg gaaaatatca cgacaggcta atctgacttc tttatcaggt
    73861 tctgcgggtt gcctcgccgc tatttcaaat gcctttggta caaccgaatc aaatttaatg
    73921 atatcggcta tgtcataaac aaatgaaagc ggtttgccac tatggataaa cccaatagcg
    73981 ggcgcatatc ccgcggctaa tactgccgct tcagatatac cgtacagaca tgatgtagca
    74041 gcactgatgc agcgattcac aacatcgcct ttttcccagt ctttaggatc gtatttgcga
    74101 ccattccatt tcacaccata ttgtttcgcc agtaatgcat acgtctggcg aacgcgagat
    74161 ccctcaattc cacgtagctg ctccactgaa cggcgagctg gcggtggctc acgaaaacgt
    74221 aattcataca ttttgcgcac caccttcaga cgtagatctt ccgttaaagc cagctttgcc
    74281 tggtagagta acttatcagc acgcgctcca ccgggttgtc cggaagagta gacgcgaaca
    74341 cccgcttcac cgacccagac ctgtagcgtc cccaccgtgg cggccagatg caccgccgcg
    74401 tgggaaactc tcgttcccgg ttcgagcata atgcaggcga ccgatcccac cggaatgtgc
    74461 gtgcggatcc cggttttatc gatcagcacg aaagcgccgt ccagtacgtc gatttgcccg
    74521 tactggagga agatcataga ggtgcgatct tttaacggga tcgggctcag cggtacaaac
    74581 gtcacgcctc tgctccgggt ttgatcagca tcagaccaca gccgaacgcg cggcttttgc
    74641 catacccctg actgagacgt tgcagaaata acccgggatc ggtgaccgta agcatccccg
    74701 tataatccac gctactgaac tggatcagtt gccgggagtt ttcccgccgc agttgctgtt
    74761 gtcggtaggc atcaaccgag atatccagca gtgtaaaacc acttctctcc ccctgcgctg
    74821 ccagccagtc cagcgccgcc tgttgttgat gcaaccagac atcacttcct tccgcctgcc
    74881 ccctcacctg ccgtttcgcc tccatcagca gatcgtggcg cttgcccgct ttacagattg
    74941 ttggattcgc ccgcaggttg aaacaaagtt gttgtccggt acacagctcg ggcgcaaatg
    75001 accggcattc tatggtgaac gtatcacttt ccgccgggcg ttcctgcgac agtacaaaaa
    75061 agcgaaacgc gccctggagt tcttcccgac gataaagaaa ttgcctttct ttgccgctag
    75121 ggaagagatc ccacagccac tgatgcatca catattcacc gcgatccacc agatgcagca
    75181 actgcgcagg cgaaagctgg ccggtatgta aggttattct tgagaggtac atggttcctc
    75241 cttgctgagc cacggcccct gattgacggt gcgttcccca aacagccact gctgacgatt
    75301 taaaggaaca tcccggcggc gtaatatttt gctcgcgacc aggccgtcgt gtttcccttc
    75361 ccaccagcat tcatcctgaa gtttcgggag tgaaactttc agtttatgga aatgatcctg
    75421 atactgttgg tatgcattac ataaggcatc acacgcgttg ccttcgagca gtaacggcgc
    75481 aagaggtaat gccagcggat gactttttcg ccccagataa agcggaaaaa ctggatggcg
    75541 taaaccgtcc tgcaactgtt cgaggctgta aggcgcatcg gctgttgttg ccaccgccac
    75601 catccaccag gcatcggtgt agtagtcgcg ccgggagata atcgcgctca aaagctcagg
    75661 gtcgctcaac tcttcgcgac ggctgaaata acgtgcttta cgcacctctt ttggcatctg
    75721 gaccgtgtga taatcccgtg cccagcgcgg gttacggctg gcgcaaacca ccagtgaata
    75781 gtggcgatta aacgcgttta agcgttcggt atcatcacgc cggatcccta ccccggcagc
    75841 cagcagcccc agcaatgctg agagcgaagg cagttcatgg gtatgacgca cttcgccggg
    75901 ggcatcgacg ccccaggatg ccatcggccc atgaagctga aaaatcagat attggctcat
    75961 aagccgcccc ttacgcgcag ataaagtcca gcacgtcctt catgcttccc tgtttgttca
    76021 tcacgtcaaa acttgcgtaa tcggtcttct gttcgtagac cgtattcata ttttcgcgaa
    76081 gagttgtaat acgctgcacc gccacctcta actgacgggt gccattaatg ggttcataga
    76141 aagctgccgc cagagaacgc ggttgatcgg tgcctttttc tgccagcgcc caggaggcat
    76201 aagcccggct ggcaaagctg ttttgtttac cggttgggga gactttaagc gcggcttccg
    76261 taaaggcgcg tatggtctga ttagctaacg cttcgtcacc gccgaggttt tctaccagca
    76321 gatctttatc aatgcagata agatataggt gtagaacagc gcagaaccga atccggtttc
    76381 atctacatga ccggcaccgg catcttcaga agcctggcgt aaatcatcta cggcggtgaa
    76441 aaaatcattt tcgacaatcg tttcactgac accaaacgca tgcgcgacct ggcaggcggc
    76501 ttctacatta aacccgggtt ttttcgccag catacgacca aacatagcga tatctaccgc
    76561 catacggtct ttacgtaaca aagcgagatc tttctctttt ggcgcgcgct tttcttctgc
    76621 cagttgatgg gccagcgctt ttacggcgtc aaattctgcc gggctgatat ggacgagctg
    76681 ttcagtttcg gcgctagtga gcggatcttt tggttttttg tcgttttttt gttttttgtc
    76741 ttttttggct ttcccaagat aatcggctat ctctactgcc cattcgatgg cttttttatc
    76801 ttcgattcct ttctcaatca ggatagttgc cgcctcacgc gcaatacgcc cgctacgaac
    76861 accaatatgg cccgccagcg cctgttcaaa aagttcagaa gtgcgccaag cacgcttcag
    76921 actttgcgag gaaacgcgca gacgcgttgc tccacccaga atcacagttt tcggagagcc
    76981 ggaatcatca cggttaagat tggcagcagg gtaagcggtt aacaaatgaa gctgaataaa
    77041 tgttgtcatg agatagtcct ttattaattt tgttcgttat cggcgtcgcc agcctggtaa
    77101 tattccagcg cccagcgccc agcgcccagc gcccagcgcc cagcggatac ggataaattc
    77161 tgttggtcgc tgctggcggc ggtgatgatt gagcaggtca tcgctctcct ggcaccagcg
    77221 gaagaccccc tcttccagag agtcaagatt gacggaaccg tttaataatt tgaccgcacg
    77281 gcgtaactgc cgcaataaat catccggcgt ttttactgcc aacaaacgag taaaccgccc
    77341 ctttttcatt accgccgcca gttgcgcggc aaaaggctgc cgttcgtcaa tagctttaac
    77401 attggcgcta actgctgcta tcagcgctag tgcggtgata cgccactcgg gttcatcctg
    77461 ccactttatt tgtctgttct taagaaacag gcgaaatcca tccgtcagac aaacatcatt
    77521 aaccgtcgta ctacgccgca gactggcacg ctcgccgcgt ttatcctgca actcttcatg
    77581 ccatttacgc agcgcaactt tatgctcatc ttttacaata ctcattcagc agcctcctgc
    77641 tttttctccc tggcggcgtt agcattttgc ttctccgctg atgttgtaaa atatttcttg
    77701 cgtgcggtca tgacgcgttc caaatcaacg ggctcatatg gattggtaaa gacacgatcg
    77761 tcaaaatcct gacgcgcgaa taaccacatt tccttttgcc atttactgag taattcgtcc
    77821 ggatcctgac cttcttcaat tttgcgcact aatctcagga atcgatgctg agttttgttc
    77881 cagaagtcaa tatccacaaa accgaaatca agctttgtgg ttttcttctc agagaaccat
    77941 gcttctttca acgctcggtg taacaagctt agaatgtgtg atgccgcttg cgctgccaga
    78001 cgcaacttag gtattaggtc ttcttttttg tttaacaaca gcgggaaatg gtgttcatac
    78061 caacaacgaa ccttcatatc ttcaaaatca aaaccaaatc cccataagcc cacccttgtc
    78121 tgctttagat tgctggcatt caaaagtttc acgacctgtg cggggagttc agtattgttt
    78181 tttgagttac ctacctctat gaggcctaac cagtcgcgcc agattaaacc accaggttgt
    78241 ggtttaactg agtaaaaatc tccaccctct ttaagcggca gacggtaagg tgttagagga
    78301 tggcgccaca tgacgtattg aacaccatag tttttgagtg acatcaagcc caaaagagca
    78361 tcgctctgcc taccgcaaat gtcgcagtta cctattgagg ttgtcttaaa atcaatacgt
    78421 atacgtcgtg gcattcccca gtaggcctga agtttattga cttgttcatc cgttaccaca
    78481 gcaccgtcca gttcgctggt acgcgtcggt gcaagccaag gaaagaccag gtcatcaaag
    78541 gtttttggta aaggtaaatc ggcttcatct tgcggcatta cgtttagcca cagctttcgc
    78601 cacaaagggg tttgttgatt gccctgatac tccagcaatt ctataagtgt cgtcagaggc
    78661 cctccgccac gtagcccagg atagtaaccg cgtccaccta taggtgcatt caactggatt
    78721 gaaaaaagcg ctaaaggcac acaatgcagg cacagatgct gagttgtgtc tcgcttaata
    78781 aaatggtctg tatttcgttc ttttgtttgt tttcctggtg cgtcaggaag aagcgaagca
    78841 ataaaagttg ccttaacttt taattcatca aaatcctgca taaatgaagg ggaatctggc
    78901 ccaaactgaa aagcatgttc taaagacagc aatgcttccc ttagcttttc agcttccagt
    78961 ccgtcttccc agatatcatc ccaactacgt tgatcttttg gcgcgaagct ggtttgtagt
    79021 agccccagca aaaactgcca ggccgccccc tggagatctg cccgcggcgc agcgatatcg
    79081 acaacatttt catcagccag atcgactggt gccagtttgc ctgtcgttcc gtctttaaaa
    79141 cgaacgggca accacggggt tgtcagaagt gaaaacgagt tcatacgcat cctctcgtaa
    79201 aaaagaccat cccaggtcgg ttgtccatcc ttccatcctt ttctgtatat caactattca
    79261 gagagtcttg tcgcagacat ctcaaccaac cttgccaatc aatccctccc tggccgaata
    79321 cccgcaactt tcgtcatcgg tgactaaaat cacgtttgcc atttccggat cttgccgctg
    79381 ttcaacgcac cactgcctga acgcatcccc ttccagtaaa gaaaactcat cccgatgttt
    79441 tttccaccag cttcggcgca ctctgacaac gctcatttcc catgcgtgag caccggtggc
    79501 ataaggcttc accacatcgt caatacaggt agccagccac agggaaacag actcttcagc
    79561 cagacgtgtc gacagttttt ccggaaggta atcgttgata ttggcggcat agccgagcct
    79621 gaagttcagc acatactttt tagcccttgc gcgatcacag taatatttgc ccacttgctc
    79681 ctgctcgctg cgggcaaatc cttccggcat taccacgtcc tcaccgtaaa ctgattcaat
    79741 aagaagccgg gctgcgtgcg gcatctgaat agcgccttgc tcacgcagca cacgctgcgt
    79801 cagccagatt cgcccatgat cgggatagac atatgcactg ttacgcatgg cactgccgaa
    79861 ccattcgtca ccaggagcgt catcccagac gggggccaga atcagcaatt caggggggga
    79921 acgctcgtct tttccgtcac gctttaactg accattaata tcgcggatat gccgctgtaa
    79981 tcgccccgct cgctggatta gcaaatcaat gggggccagg tcggagatca tttcgtccag
    80041 gtcaatatcc acgctttgtt caattacttg tgtacaaaca atgacttgag acgctctctg
    80101 taaactacaa tatttaccaa agcgtgccag cgtttccgtt tcaattcgct ggcgatcgct
    80161 aaaagcaaag cggctatgaa aaagtgaaag gctggaagca ggaatgacgc cgcgggcaag
    80221 cagctgacga tacacctgaa tagcgtcatc aacagaattc cgaatccagg cgatgcattt
    80281 cccctgactt accgccgatt cgatacgcgc aatacactct tgttcactat ggagccaacc
    80341 cacgctcacg ctacgttcaa cttctttgcg cgtcgctacc cggtgcgaat gtacatcggt
    80401 tttcgtgaca tgcgtcagcc aggggtaatc atcttttccc aggagtgggg cttcttgctg
    80461 gccctctgca ccacgcgcaa aggcggcgac gagtttgtcg cgctgctgtt gagataacgt
    80521 agcagaaagc aaaataacgc tgtttccgcc acgcgcctgc cgctcgatca gcccttcaag
    80581 aatgcacgac atataagcgt cacaggcgtg gatctcatca gccagcagga ttttgttact
    80641 cagccccaga agccgcagat tattatgttt aaacggcatc actgccatca tcgcctgatc
    80701 cagggtgccg acgccaattt cagccagcaa cgccttcttg ttactgtcgg caaactaggc
    80761 cgcacaaccc tggctgaaag cttgttcatc cggttcttct gacccgatta aatcaccgga
    80821 ccagagtgat tcatta
//
LOCUS       DAJTOR010000003        66204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTOR010000003 DAJTOR010000000
VERSION     DAJTOR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.003"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="OO249_000179"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="OO249_000179"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5634607.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQDRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OO249_000180"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OO249_000180"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5634608.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OO249_000181"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OO249_000181"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5634609.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OO249_000182"
     CDS             complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OO249_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5634610.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OO249_000183"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OO249_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5634611.1"
                     /translation="MEFSPLLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OO249_000184"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OO249_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5634612.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="OO249_000185"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="OO249_000185"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5634613.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQSPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7606..10128
                     /gene="mrcB"
                     /locus_tag="OO249_000186"
     CDS             7606..10128
                     /gene="mrcB"
                     /locus_tag="OO249_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5634614.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWAGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10348..12537
                     /gene="fhuA"
                     /locus_tag="OO249_000187"
     CDS             10348..12537
                     /gene="fhuA"
                     /locus_tag="OO249_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5634615.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12588..13385
                     /gene="fhuC"
                     /locus_tag="OO249_000188"
     CDS             12588..13385
                     /gene="fhuC"
                     /locus_tag="OO249_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5634616.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSSKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13385..14275
                     /gene="fhuD"
                     /locus_tag="OO249_000189"
     CDS             13385..14275
                     /gene="fhuD"
                     /locus_tag="OO249_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5634617.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14272..16254
                     /gene="fhuB"
                     /locus_tag="OO249_000190"
     CDS             14272..16254
                     /gene="fhuB"
                     /locus_tag="OO249_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5634618.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATSTMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16289..17569)
                     /gene="hemL"
                     /locus_tag="OO249_000191"
     CDS             complement(16289..17569)
                     /gene="hemL"
                     /locus_tag="OO249_000191"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5634619.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17794..19215
                     /gene="clcA"
                     /locus_tag="OO249_000192"
     CDS             17794..19215
                     /gene="clcA"
                     /locus_tag="OO249_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5634620.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSEILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19239..19304
                     /locus_tag="OO249_000193"
     CDS             19239..19304
                     /locus_tag="OO249_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5634621.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19297..19641
                     /gene="erpA"
                     /locus_tag="OO249_000194"
     CDS             19297..19641
                     /gene="erpA"
                     /locus_tag="OO249_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5634622.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19688..20311)
                     /locus_tag="OO249_000195"
     CDS             complement(19688..20311)
                     /locus_tag="OO249_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5634623.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20349..21148)
                     /gene="btuF"
                     /locus_tag="OO249_000196"
                     /pseudo
     CDS             complement(20349..21148)
                     /gene="btuF"
                     /locus_tag="OO249_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
     gene            complement(21141..21839)
                     /gene="mtnN"
                     /locus_tag="OO249_000197"
     CDS             complement(21141..21839)
                     /gene="mtnN"
                     /locus_tag="OO249_000197"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5634624.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFEADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21923..23440
                     /gene="dgt"
                     /locus_tag="OO249_000198"
     CDS             21923..23440
                     /gene="dgt"
                     /locus_tag="OO249_000198"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5634625.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23570..24994
                     /gene="degP"
                     /locus_tag="OO249_000199"
     CDS             23570..24994
                     /gene="degP"
                     /locus_tag="OO249_000199"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5634626.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25149..26306
                     /gene="cdaR"
                     /locus_tag="OO249_000200"
     CDS             25149..26306
                     /gene="cdaR"
                     /locus_tag="OO249_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR5634627.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGRLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIYRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26395..26781)
                     /locus_tag="OO249_000201"
     CDS             complement(26395..26781)
                     /locus_tag="OO249_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5634628.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26943..27755)
                     /gene="yaeI"
                     /locus_tag="OO249_000202"
     CDS             complement(26943..27755)
                     /gene="yaeI"
                     /locus_tag="OO249_000202"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR5634629.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27810..28634)
                     /gene="dapD"
                     /locus_tag="OO249_000203"
     CDS             complement(27810..28634)
                     /gene="dapD"
                     /locus_tag="OO249_000203"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5634630.1"
                     /translation="MQQLQNIIETAFERRAEITPTNADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGHVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28665..31337)
                     /gene="glnD"
                     /locus_tag="OO249_000204"
     CDS             complement(28665..31337)
                     /gene="glnD"
                     /locus_tag="OO249_000204"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5634631.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31399..32193)
                     /gene="map"
                     /locus_tag="OO249_000205"
     CDS             complement(31399..32193)
                     /gene="map"
                     /locus_tag="OO249_000205"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5634632.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32561..33286
                     /gene="rpsB"
                     /locus_tag="OO249_000206"
     CDS             32561..33286
                     /gene="rpsB"
                     /locus_tag="OO249_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5634633.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33544..34395
                     /gene="tsf"
                     /locus_tag="OO249_000207"
     CDS             33544..34395
                     /gene="tsf"
                     /locus_tag="OO249_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5634634.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34542..35267
                     /gene="pyrH"
                     /locus_tag="OO249_000208"
     CDS             34542..35267
                     /gene="pyrH"
                     /locus_tag="OO249_000208"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5634635.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35559..36116
                     /gene="frr"
                     /locus_tag="OO249_000209"
     CDS             35559..36116
                     /gene="frr"
                     /locus_tag="OO249_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5634636.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36208..37404
                     /gene="ispC"
                     /locus_tag="OO249_000210"
     CDS             36208..37404
                     /gene="ispC"
                     /locus_tag="OO249_000210"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5634637.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37592..38350
                     /gene="ispU"
                     /locus_tag="OO249_000211"
                     /gene_synonym="uppS"
     CDS             37592..38350
                     /gene="ispU"
                     /locus_tag="OO249_000211"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5634638.1"
                     /translation="MLSATQLLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFIDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38363..39220
                     /gene="cdsA"
                     /locus_tag="OO249_000212"
     CDS             38363..39220
                     /gene="cdsA"
                     /locus_tag="OO249_000212"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5634639.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVSLLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39232..40584
                     /gene="rseP"
                     /locus_tag="OO249_000213"
     CDS             39232..40584
                     /gene="rseP"
                     /locus_tag="OO249_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5634640.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40614..43046
                     /gene="bamA"
                     /locus_tag="OO249_000214"
     CDS             40614..43046
                     /gene="bamA"
                     /locus_tag="OO249_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5634641.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43168..43653
                     /gene="skp"
                     /locus_tag="OO249_000215"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43168..43653
                     /gene="skp"
                     /locus_tag="OO249_000215"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5634642.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43657..44682
                     /gene="lpxD"
                     /locus_tag="OO249_000216"
     CDS             43657..44682
                     /gene="lpxD"
                     /locus_tag="OO249_000216"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5634643.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44787..45242
                     /gene="fabZ"
                     /locus_tag="OO249_000217"
     CDS             44787..45242
                     /gene="fabZ"
                     /locus_tag="OO249_000217"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5634644.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45246..46034
                     /gene="lpxA"
                     /locus_tag="OO249_000218"
     CDS             45246..46034
                     /gene="lpxA"
                     /locus_tag="OO249_000218"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5634645.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            46034..47182
                     /gene="lpxB"
                     /locus_tag="OO249_000219"
     CDS             46034..47182
                     /gene="lpxB"
                     /locus_tag="OO249_000219"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5634646.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVARDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47179..47775
                     /gene="rnhB"
                     /locus_tag="OO249_000220"
     CDS             47179..47775
                     /gene="rnhB"
                     /locus_tag="OO249_000220"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5634647.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47812..51294
                     /gene="dnaE"
                     /locus_tag="OO249_000221"
     CDS             47812..51294
                     /gene="dnaE"
                     /locus_tag="OO249_000221"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5634648.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGVNAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYASGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51307..52266
                     /gene="accA"
                     /locus_tag="OO249_000222"
     CDS             51307..52266
                     /gene="accA"
                     /locus_tag="OO249_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5634649.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52365..54474
                     /gene="ldcC"
                     /locus_tag="OO249_000223"
                     /pseudo
     CDS             52365..54474
                     /gene="ldcC"
                     /locus_tag="OO249_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            54531..54920
                     /locus_tag="OO249_000224"
     CDS             54531..54920
                     /locus_tag="OO249_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5634650.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54985..56283
                     /gene="tilS"
                     /locus_tag="OO249_000225"
     CDS             54985..56283
                     /gene="tilS"
                     /locus_tag="OO249_000225"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5634651.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56332..56592)
                     /gene="rof"
                     /locus_tag="OO249_000226"
     CDS             complement(56332..56592)
                     /gene="rof"
                     /locus_tag="OO249_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5634652.1"
                     /translation="MLMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56579..56779)
                     /locus_tag="OO249_000227"
     CDS             complement(56579..56779)
                     /locus_tag="OO249_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5634653.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56945..57490
                     /locus_tag="OO249_000228"
     CDS             56945..57490
                     /locus_tag="OO249_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5634654.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57487..57909
                     /gene="arfB"
                     /locus_tag="OO249_000229"
     CDS             57487..57909
                     /gene="arfB"
                     /locus_tag="OO249_000229"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5634655.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSAKAMRGKVRSGRE"
     gene            57923..58633
                     /gene="nlpE"
                     /locus_tag="OO249_000230"
                     /gene_synonym="cutF"
     CDS             57923..58633
                     /gene="nlpE"
                     /locus_tag="OO249_000230"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5634656.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58833..59653)
                     /locus_tag="OO249_000231"
                     /pseudo
     CDS             complement(58833..59653)
                     /locus_tag="OO249_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(59706..61424)
                     /gene="proS"
                     /locus_tag="OO249_000232"
     CDS             complement(59706..61424)
                     /gene="proS"
                     /locus_tag="OO249_000232"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5634657.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61535..62242)
                     /gene="tsaA"
                     /locus_tag="OO249_000233"
     CDS             complement(61535..62242)
                     /gene="tsaA"
                     /locus_tag="OO249_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5634658.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62239..62643)
                     /gene="rcsF"
                     /locus_tag="OO249_000234"
     CDS             complement(62239..62643)
                     /gene="rcsF"
                     /locus_tag="OO249_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5634659.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62761..63576)
                     /gene="metQ"
                     /locus_tag="OO249_000235"
     CDS             complement(62761..63576)
                     /gene="metQ"
                     /locus_tag="OO249_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5634660.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63616..64269)
                     /gene="metI"
                     /locus_tag="OO249_000236"
     CDS             complement(63616..64269)
                     /gene="metI"
                     /locus_tag="OO249_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5634661.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64262..65293)
                     /gene="metN"
                     /locus_tag="OO249_000237"
     CDS             complement(64262..65293)
                     /gene="metN"
                     /locus_tag="OO249_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5634662.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65481..66053
                     /gene="gmhB"
                     /locus_tag="OO249_000238"
     CDS             65481..66053
                     /gene="gmhB"
                     /locus_tag="OO249_000238"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5634663.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15717 a        16406 c        18171 g        15910 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taattcgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgatcctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgagcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtgga
      481 gtgcggtaaa acgaagaaac ggcaagaatg tggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgc ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacggcgac
      661 gcggtgacgg ctcttcatcc aactcgttaa gcatcccttt ttgatctggt ggcgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcgttacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt catgccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtt gcaacttcga taatctccgg gccaaacatc acatgagcca
     1681 gacggaaacg gcgacccaca aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttattgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcgtccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctacctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgccgcg
     2521 cagctggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgacaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgttcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttctgc
     4021 gccatatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttagtt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc cacgcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaagggg agagaattcc attgcgacaa tccttgttat
     4381 tgcattagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacctggcgg cgggattgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggcggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctcggcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccagt ggataatctg ttcgcggatt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtttta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggaggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtagttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggagt tgagcggtgt tggattggtg
     5341 atccttgatg aatttcatga gcgtagcttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctaa ttatgtcggc tacgctggac
     5461 aacgaccgct tgcagcaaat gctgccagaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgatgccgtt
     5581 gcggtagcca ccgctgaaat gctgcgtcag gaaagcggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg tgtgcaggaa caactggctt cgcgcatcgg cagtgatgta
     5701 ttgctctgcc cgctgtatgg cgcgttgtcg ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ttttgatccg
     5881 cgcacggggc taacgcgact gattactcaa cgcgttagcc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atctgcctgc atctaatcgc caaagaacaa
     6001 gcagaacgcg ctgccgcgca aagtgaaccg gaaatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca ggggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttagcgc gaagaacgac gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgtggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcgtcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctgccgg tcgatattga tgagttagta
     6661 caacgctgcc cgcagctggt acagcagtct gacactgtgg agtgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctgcaaatc ggtctgctga cggtgaaagt gcagccgctg
     6781 gcgaaaccct cggaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt attggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgact ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaagaga tgtttggcga ggccaccaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctggagt tgctttcacc tgcccaaagt
     7261 ccattacaaa tcacacgaga tttgagcgcc ttctggaaag gagcgtaccg tgaggtgcaa
     7321 aaagagatga aagggcgtta tcccaaacat gtctggccgg acgacccggc aaatactgca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc tccctcaagg
     7501 gagaggggac cgatcgaaca caaattttga gagatatctt cttctgtctt gtaacagaag
     7561 aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac
     7621 cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt
     7681 cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg
     7741 atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg
     7801 ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt
     7861 tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcctgcggca
     7921 gtttatggcc gaatggtcaa tcttgagcca gatatgacca tcagcaagaa cgagatggtg
     7981 aagctgctgg aggcgactca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt
     8041 accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa
     8101 gaagggcagg tgcgtgcgca tctgaccttt gatggtgatc atctggcgac catcgtcaat
     8161 atggagaaca accgtcagtt cggctttttc cgtcttgatc cgcgtctgat caccatgatc
     8221 tcctcgccaa acggtgagca acgtctgttt gtgccgcgca gtggtttccc tgatttgctg
     8281 gtggatactt tgctggcgac agaagaccgc catttctacg agcatgatgg gatcagcctc
     8341 tactcaattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg
     8401 agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ctcttactgg
     8461 cgtaaagcga acgaagccta catggcgctg atcatggacg cgcgttacag caaagaccgt
     8521 attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc
     8581 ggcttcccgc tggcaagctt gtattacttt ggtcgcccgg tagaagagct aagcctcgac
     8641 cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac
     8701 ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt
     8761 attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca gccgcgcggt
     8821 ggggtgatct ctcctcagcc agcctttatg caactggtgc gtcaggagct gcaggcaaaa
     8881 ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg
     8941 gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt
     9001 aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt
     9061 gcgatggtgg gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt
     9121 cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa
     9181 atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc
     9241 ctggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg
     9301 gtggatgcgt tgacccgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg
     9361 ctgcctgcag ttacggatac ctggatcaaa ctgggcgtac cgaaagatca gctgcatccg
     9421 gttccggcga tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcgttc
     9481 cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg
     9541 gaagatggca aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag
     9601 gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag
     9661 cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac
     9721 gtagatactt ggtttgcagg cattgacggc agcacggtga ccatcacctg ggcaggccgt
     9781 gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt
     9841 tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat
     9901 atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc
     9961 tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc
    10021 aatccgtttg atcagtcttc tcagccgcaa cagcaacctg ctcagcaaga gcagaaagac
    10081 agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaaca tctaagcgtg
    10141 aaataccgga tggcgagttg ccatccggta aaataacatc ccatctaaga tattaaccct
    10201 ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt accgcttgcg
    10261 aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg tttacgttat
    10321 cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca gccaaaacac
    10381 tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc tgtttatgca
    10441 caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc tgcgccgcaa
    10501 gaaagcgcat gggggcctgc tgcaactatt gcggcgaagc actctgctac tgcgactaaa
    10561 acggatacac caattgaaaa aacgccgcag tctatttcgg ttgtgacaaa tgaagagatg
    10621 cagatgcatc aatttcagtc tgtaaaagaa gcattaggtt atacacctgg cgttacggtt
    10681 agcagtcgtg gtgcttctaa tacatatgat tttgttatca ttcgtggttt ctcatctgtt
    10741 ggtctgaatc aaaataatta ccttgatgga ctaaaacttc agggcaactt ttataacgat
    10801 gctgtgattg atccttacat gctcgagagg gttgaactga tgcgtgggcc gacgtctgtt
    10861 ctctatggaa aaagtaatcc tggtgggatt atttctatgg tgagtaagcg tccgaccact
    10921 gaacccctga aagaaattca gtttaaaatg gggacggata atctgtttca gaccggattc
    10981 gatttcagtg atgcactgga tgataacggt gagttctctt accgtttgac gggcctcgct
    11041 cgctcaacaa acgaacagca gaaaaactct gaatctcagc gttataccat tgctccatca
    11101 ttctcatggc gtccagacga caaaaccaat tttactttcc tgtcctattt ccagaatgaa
    11161 ccagaaacgg gttattacgg ttggttgccg aaagagggga ccgttgagcc attgcctaat
    11221 ggtaagcgtc taccgactga cttcaatgaa ggtgcatcga ataatacata ctcccgtaac
    11281 cagaaaatgg tgggatatag ttttgaacat ggttttaatg acaccttcac cgtgcgtcag
    11341 aatctgcgtt tcagtgaaat gaaaacctca cagaaaagtg tttatggcac agggattgct
    11401 ggtgatggtc ataccctaaa ccgcgggaca gtggtggata atgagcgtct acaaaacttt
    11461 agcgttgata cccaacttga aagtaagttt gctacaggtg acgtcgagca tactttgctg
    11521 acaggggtag acttcatgcg gatgcgtaat gatatcaatg ccagctttgg atccgcacca
    11581 tccatcgatc tttataacaa atatcaccct gaatactttg catttggtag cgcagagcca
    11641 taccagatga atgaaagcaa acaaacaggt atttatgttc aggatcaggc ggaatggaat
    11701 aaatgggtat tcactctggg gggacgctac gattggtcta agcaagcgac cactgttcgt
    11761 gaaaactctt atacgccgac tgaaggttat attgagcgca atgatcatca gttcacctgg
    11821 cgcggtggtg taaattactt attcgataat ggtatttcac cttactttag ctatagccag
    11881 tcctttgaac cgagtgcttt cgatctgtgg agcaacccac gcatttccta taagccatcg
    11941 aaaggtgaac agtatgaagc tggcgtaaaa tatgttccga atgatatgcc ggtcgttgtt
    12001 acgggcgcag tctatcaatt gacgaaaaca aataacctga cagcagaccc aacaaaccca
    12061 ttagcgcaag tcccagcagg tgagattcgc gctcgtggtg tggaacttga agcaaaagcg
    12121 gcgttaaatg ccaatattaa cttgacggct tcttatacct acaccgatgc ggaatacacc
    12181 aaagacacca atctcaaagg taaaactcca gaacaagtac cggagcatat ggcatctctc
    12241 tggggggatt ataccttcaa tgaagggccg ctttctggtt taacattggg aacaggtggt
    12301 cgttttattg gttccagcta tggtgatccg gcaaacactt ttaaagtggg tagcgcagct
    12361 gtaatggatg ctgttgtaaa atatgatctg gcacgctttg gtatggcggg atccagcctt
    12421 gctgtgaacg tcaacaattt gctcgatcgt gagtatgttg ccagttgctt ccagacttat
    12481 ggctgcttct ggggcgcaga acgtcaggtc gttgcaaccg caaccttccg tttctaattt
    12541 ctcttttggg gcacggattt ccgtgcccat ttcacaagtt ggctgttatg caggaataca
    12601 cgaatcattc cgataccact tttgcactgc gtaatatctc ctttcgtgtg cccgggcgca
    12661 cgcttttgca tccgctgtcg ttaacctttc ctgccgggaa agtgaccggt ctgattggtc
    12721 acaacggttc tagtaaatcc actctgctca aaatgcttgg ccgtcatcag cctccgtcag
    12781 aaggggagat tcttcttgat gcccagccgc tggaaagctg gagcagcaaa gcgtttgccc
    12841 gcaaagtggc ttatttgccg cagcagcttc ctccggcaga agggatgacc gtgcgtgaac
    12901 tggtggcgat tggtcgttac ccgtggcatg gcgcgctggg gcgctttggg gcggcagatc
    12961 gcgaaaaagt cgaggaagct atctcgctgg ttggcttaaa accgctggcg catcggctgg
    13021 ttgatagtct ctctggcggc gaacgtcagc gggcgtggat cgccatgctg gtggcgcagg
    13081 atagccgttg tctgttgctc gacgaaccga cctcggcgct ggatatcgcc caccaggttg
    13141 atgtgctggc gctggtgcac cgtttaagtc aggagcgtgg cctgacggtc attgccgtgt
    13201 tgcacgatat caatatggcg gcacgctact gtgattatct ggtcgccctg cgcggtggtg
    13261 aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg cgataccctc gaaatgattt
    13321 atggcatccc gatgggtatt ttgccgcatc cggcgggtgc tgcacctgtg agttttgttt
    13381 attgatgagc ggcttacctc ttatttcgcg ccgtcgactg ttaacggcga tggcgctttc
    13441 tccgttgtta tggcagatga ataccgccca cgcggcggct attgatccca atcgtattgt
    13501 ggcgctggag tggttgccgg tggaattact gctggcgctc ggcatcgtgc cttacggcgt
    13561 agcggatacc atcaactatc gcctgtgggt cagcgaacca ccattgccgg actcagtgat
    13621 cgacgtcggt ttgcgcacag aacctaacct tgaactgctg accgaaatga aaccgtcgtt
    13681 tatggtctgg tcggcaggat atggcccttc agcagaaatg ctggcacgta ttgcgccggg
    13741 gcgtggattt aacttcagcg acggcaaaca gccgctggcg atggcgcgta aatcactgac
    13801 ggaaatggca gatttactta acctgcaaag cgcagcagaa acgcatttag cgcaatatga
    13861 agactttatc cgcagcatga aaccccgctt tgtgaagcgt ggtgcacgcc cgttattgct
    13921 gacgacgctt atcgatccgc gccatatgtt ggtcttcggt ccaaacagct tgttccagga
    13981 aattcttgat gagtacggca tcccaaatgc ctggcaaggg gaaaccaact tctggggcag
    14041 taccgccgtc agtatcgatc gtctggcagc gtataaagac gttgatgtgc tctgttttga
    14101 tcacgacaac agcaaagaca tggatgcgct aatggcaacc ccgctgtggc aggccatgcc
    14161 gtttgtccgc gccggacgct ttcagcgcgt acccgcagtc tggttttatg gtgcgacgct
    14221 ctcggcaatg cactttgtgc gcgttctgga taacgccatc ggaggtaaag cgtgagtaaa
    14281 cgaattgcgc ttttcccggc gttattgctg gcgctgctgg tgattgtcgc tacggcgctg
    14341 acctggatga acttttcgca ggcgctgccg cgtagccagt gggcgcaggc cgcctggtcg
    14401 ccggatattg acgtcatcga gcagatgatt tttcactaca gcttgctgcc gcgtctggcg
    14461 atttcgctgc tggtgggcgc aggcctgggg ctggtgggcg tgctgtttca gcaagtgctg
    14521 cgtaatccgc tggcggaacc gacgacactt ggcgttgcta caggcgcgca actggggatt
    14581 accgtcacta cgctctgggc gatcccaggg gcgatggcga gtcagtttgc tgcgctggcg
    14641 ggcgcttgcg ttgttggctt aatcgtcttt ggcgtcgcgt gggggaaacg gctttcgccg
    14701 gtaacgctga tcctcgcggg gctggtagtg agcctttatt gtggggcaat caatcagtta
    14761 ctggttatct tccaccatga ccaactgcaa agcatgttcc tgtggagcac cggaacgctg
    14821 acgcaaaccg actggggcgg tgttgagcgt ttatggccgc agttgctggg cggtgtgatg
    14881 ctgacgttgc tgctacttcg cccgttaacc ctgatggggc ttgatgatgg cgtggcgcgc
    14941 aatctcgggc tggccttgtc gctcgcgcgt ctggcggcgc tgtcgctggc gattgtcatc
    15001 agtgcgctgc tggtgaacgc ggtggggatt atcggcttta tcgggttgtt tgcgccgttg
    15061 ctggcaaaaa tgctgggggc gcggcgtctg ctgccgcgac tgatgctggc gtcgttgatt
    15121 ggtgcgctga tcctctggct ttccgatcaa atcatcctct ggctgactcg cgtgtggatg
    15181 gaagtgtcca ccggttcggt cactgcgttg atcggtgcgc cgctgctact gtggctgttg
    15241 ccgcgtttac gcagcattag cgcgccggat atgaaggtca acgatcgtgt cgcggctgaa
    15301 cgccaacatg tgctggcgtt tgccctcgcg ggcggcgtgc tgctgttgat ggctgtggtg
    15361 gtggcgctgt cgtttggtcg tgatgcgcac ggctggatgt gggcgagcgg ggcgttgctc
    15421 gatgatttaa tgccctggcg ctggccgcga attatggcgg cgctatttgc gggcgtcatg
    15481 ctggcggtgg cgggctgtat tattcagcga ctgaccggca acccgatggc aagcccggaa
    15541 gtgctgggga ttagctccgg cgcagcgttt ggcgtggtac tgatgctgtt tctggtaccg
    15601 ggtaatgctt ttggctggct gttacccgcg ggaagtatcg gtgccgcggt gacgctgttg
    15661 attattatga tcgccgccgg gcgcggtgga ttttccccac accgcatgtt actggcgggg
    15721 atggcgttaa gtaccgcatt caccatgctt ttaatgatgt tgcaggcgag tggtgacccg
    15781 cgaatggcgc aagtgctcac ctggatctct ggttcgacct acaacgccac cgatgcgcag
    15841 gtctggcgta ccggaattgt gatggtgatt ttgctggcga ttaccccgct gtaccgccgc
    15901 tggctgacca ttttacctct gggcggtgat accgcacgag ctgtgggaat ggcgctgacg
    15961 ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga cggcgacctc gacgatgact
    16021 attggaccgt tgagttttgt tggtttaatg gcaccgcata ttgcgcggat gatgggtttt
    16081 cgacggacga tgccacacat cgtaatttcg gcgctggtgg gtggtttact gctggtattc
    16141 gctgactggt gtgggcggat ggtgctgttt ccattccaga tcccggcggg gctgctgtcg
    16201 acctttatcg gcgcgccata ttttatctat ttgttgagaa agcagagccg ttaattgagt
    16261 gcctcgcccg gtgggcgagg caaaattatc acaacttcgc aaatacccga cgtgcagcat
    16321 cgatggtgtt attgatatct tccatgctgt gcgccacaga cataaagccc gcttcaaacg
    16381 ctgacggtgc caggtaaaca ccttcgtcca gcatcatatg gaagaaacgc ttaaagcgtt
    16441 ccacgtcaca ggccatcaca tcctgatagc acgtcacgga ctcggcgtcg gtaaagaaaa
    16501 taccgaacat gccgccaacg tggttaacga ccagcggagt tccggcttct tctgccgctt
    16561 ccagcagacc ttctgccaga cgtgttgtca gctcatccag cgtttcgtga acgcccggct
    16621 gcgcgacttc attcagacag gcgaaacccg ctgccatcgc aatcgggtta ccggaaagcg
    16681 tacccgcctg atagaccgga cccgtcgggg ccagcgcatc cattacatca cgacgaccac
    16741 cgaatgcgcc taccggcatt ccaccgccga tgattttgcc gaggcaggtt aaatctggca
    16801 ctacgccgta ataatcctgt gcgccagcca gtgccacgcg gaagccggtc atcacttcat
    16861 cgatgatcag caacgcgcca aattcgtcgc acagcgcacg cagacctggc aggaactctg
    16921 gctgcggcgg aacacagttc atattgcctg ccaccggctc gacgataata caggcaatct
    16981 cttgcgggta ttgctcaaac gcggcgcgta cagaagccag atcgttataa gtacaggtta
    17041 aggtatgttt ggcgaaatct gccggaacac ccggcgagtt tggctggcct aacgtgagtg
    17101 cgccagaacc ggctttcacc agcaggcagt cagcgtgacc gtggtaacaa ccttcaaatt
    17161 taatgatttt gtcgcgaccg gtaaaaccac gggccaggcg gatggcgctc atggtcgctt
    17221 cagtgccgga gttcaccatg cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt
    17281 gcgccatttt cacttccatt tcggtcggtg caccaaagct taaaccacgc tcggcggctt
    17341 caatcacggc attgcggata gccggatggt tatggcccag caccatcggc ccccaggaac
    17401 cgacataatc gatataggct ttgccatcaa catcgtacag ataagcgccg tccgcttttt
    17461 cgataaacag tggagtgccg cccacgccag taaaggcgcg aacaggggag ttcacaccgc
    17521 cagggagcag ctcgcgcgct gcgctgtaaa gattttcaga cttactcatg gagggttcct
    17581 gattcgtaga aaaagtgaat ggctgctatt ctatgttatt cataacaagt taaatactcg
    17641 tcaaacatca ggctgcttgt actggtgcaa tcctgatttc ttagagtata aaagttttgt
    17701 gcatttgaaa cattacgctt tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc
    17761 atcttatttg tatgaccaat aagtgatcat tggatgaaaa ctgatactcc ctctttagaa
    17821 acaccgcagg ccgcgcgcct gcgacgcaga caactgattc gccaacttct tgagcgcgat
    17881 aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt cgggctggca
    17941 gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatggg ggcgctggta
    18001 catactgctg ataattatcc gcttctgtta accgtcgctt ttctctgttc ggcggtgctg
    18061 gcgatgttcg gctacttttt ggtgcgcaaa tacgcgccgg aagcaggtgg ttcggggatc
    18121 ccggaaattg aaggggcgct ggaagatcaa cgtcccgttc gctggtggcg tgtgttgccg
    18181 gtgaaattct ttggcgggct ggggacactc ggcggaggca tggtgctggg gcgcgaaggg
    18241 ccaaccgtgc agatcggcgg caatatcggt cgcatggtac tcgatatttt ccgcctgaaa
    18301 ggtgatgaag ctcgccatac gctgctggca accggtgctg ctgcggggct ggctgcggcc
    18361 tttaacgcgc cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc
    18421 tatacgttaa tttcgattaa agcggtattt attggcgtca ttatgtcgac cattatgtac
    18481 cggattttta atcatgaagt tgcgttgatt gacgtcggta aactttctga tgcgccgctt
    18541 aatacgctgt ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt
    18601 aataaatggg tgctggggat gcaggatttg ctgcaccgtg tgcacggcgg caatattacc
    18661 aaatgggtgc taatgggcgg tgcgattggc ggtctgtgtg gattgctggg gtttgtggca
    18721 ccagcaacgt cgggcggcgg ttttaacctg attcctatcg ctaccgcagg gaatttcagc
    18781 atgggaatgc tggtgtttat cttcgtcgcg cgggtcatta ccaccttact gtgcttctct
    18841 tccggcgcgc cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc
    18901 gctttcggaa tggttgtcgt tgagctgttt ccgcaatatc accttgaggc ggggactttt
    18961 gctattgccg gaatgggggc gttactggcg gcatctattc gcgcgccgtt aacggggatc
    19021 attttggttc tggagatgac cgataactac cagctcattt tgccaatgat tattaccggt
    19081 cttggcgcaa cactattagc gcaatttacc ggcgggaaac cgctatattc ggagattctt
    19141 gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagcc
    19201 agcgagaata cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag
    19261 aatttgccca attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg
    19321 ctggagttta ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac
    19381 ccgaatctga aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt
    19441 ttcacctttg atgatcaggt gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc
    19501 ctggtggttg atccgatgag cctgcaatat ctggtcggtg gttccgttga ctataccgaa
    19561 ggactggaag gttctcgttt catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc
    19621 ggttcttcct ttagcatcta atctgttgtt catgtgccgg ataaactatc cggcacatct
    19681 tctctcttca acgcccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa
    19741 tagccgccag ccgaatcaat agcgtcacga ccatgcccat catactggct gtttccagtg
    19801 gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagttg
    19861 cgtagatttc tgtacgtaaa atcatgggga tttcgcgggc cagtacatca cgaatgatcc
    19921 cgccgccaac gccagtaatg acgcccatac aaaccgcgat taacggaccg gcttccgcat
    19981 taaaggcttt attcacgcca atgccgacaa acaccgccag accaacggcg tccagcaccg
    20041 gcaacatcca ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctgg
    20101 ttaccattgc aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca
    20161 gcgccatgtc gcgaattgtc ccgccgccta cagcggtaac cacgcccagt accagaacac
    20221 caaaagggtc catacgcaat tttccggcta acaaaacgcc ggagatggca aataccgctg
    20281 tgccgactat atccagccaa tagacgagca ttgttcaatc cccactgagc acctaaaaag
    20341 gtcaggcgct aatctacctg tgaaagcgca ttacagagct gttgtgcagc gaggataata
    20401 cgtgggcttg cacgtcaaac cagtcactcg tgagaggaat aacgggaatt ttgagctgtt
    20461 caccccagta ttgtttgatt ttaggaattt ggtccggtcc gcctgtaatg acaatcgcct
    20521 gtggcgagcg tgctaacacc tgttcgcggc taacttgcgg ccaaggaacc cggctgtctt
    20581 taaagatgtt ttctccgcca caaacttcga gtacctggtt ttgaatcgac tcttttccac
    20641 tggtaaatgg cggattaatg ccgaattgca gaaaaacacg ttttttaggt ttatcagcat
    20701 attgcgcttt caattgcgcg tactgatcca gcagggattg cgcggcttgt tcggccttat
    20761 ccggttgcgg actccagggg gccagttgac gtaacgcatt ggcaatttgt tcaatgcttg
    20821 tcgcatcgac ccacatcact tttattcccg gcgaagccag ctggtcaacc tgccgctcgg
    20881 cattacctcc acgccaggca atcaccagat cgggtttcag cgcgacaatg cgttccagat
    20941 tcatcccctg ccaggtggaa acctgctcaa tcttttgcgc ttgtggagga tagtcggaat
    21001 agctgctgac cccaaccggc gtgatcccgg cggcaaaggc aagttcagtg ttggcgggag
    21061 aaagcgtgat gacgcgcggt gccgcgttga gccacagtgg cgcgagaaaa gacagggcga
    21121 ccagcgccct gaacagtgac ttagccatgt gccagtttct gcaccagtga ctcaaccatc
    21181 aggctggact gtttagcggc aacagccagg aactcatcga agctaagatg agactgttga
    21241 tcggccacgt cggagatggc gcgtacgaca acaaacggga cgttgaaatt gtggcagaca
    21301 tgggcgattg ccgtcgcttc catctctaca gcaatggcct gtgggaagtt gtggcggatt
    21361 ttcgccagac caacagaacc gttgatgaaa gcgtcgccgc taacaatcag gccacgtaca
    21421 gcgttaagat tcagttcggc aatgcaggcc tcagcggcag cgatcagttt atcgtcagct
    21481 tcaaagcctg ccggacagcc tggtaactga ccgtattcat aaccaaatgc cgtgacatcc
    21541 gcgtcgtgat aacgtgcttc gtccgagaca acgatatcgc ccactttcaa cgttggtgcc
    21601 aggccaccgg cagaaccggt gttaataatc acatctggct tgcagtgttc caacagcaaa
    21661 gtggcaccca gcgccgcagc gactttaccg atgcccgatt tcagaagcgc aacctcggtt
    21721 ccattcagtt ggccggtata gatttcgcaa ccgccgagac tgatagtttg acgattttcg
    21781 attttgtcac gcagcagcgt aacttcttct tccattgcac caatgatgcc gattttcata
    21841 gatttactcg cgataagccc gatttgaagg catagtttac catgcgctta cggggaagcg
    21901 tatttctcac gcgggagagg acatggcaca gattgatttc cgaaaaaaaa taaactggca
    21961 tcgtcgttac cgttcaccgc agggcgttaa aaccgaacat gagatcctgc ggatcttcga
    22021 gagcgatcgc gggcgtatca tcaactctcc ggcaattcgt cgtctgcaac aaaagaccca
    22081 ggtttttcca ctggagcgca atgccgccgt gcgcacgcgt cttacccact cgatggaagt
    22141 ccagcaggtg gggcgctaca tcgccaaaga aattctaagc cgtatgaaag agcttaaatt
    22201 actggaagca tacggcctgg atgaactgac cggccccttt gaaagcattg ttgagatgtc
    22261 atgcctgatg cacgatatcg gcaatccgcc gtttggtcat tttggcgaag cggcgataaa
    22321 tgattggttt cgccagcgtt tgcacccgga agatgccgaa agccagcctc tgactgacga
    22381 tcgctgcagc gtggcggcac tacgtttacg ggacggggaa gaaccgctta acgagctgcg
    22441 gcgcaagatt cgtcaggact tatgtcattt tgaggggaat gcacaaggca ttcgtctggt
    22501 gcatacattg atgaggatga atctcacctg ggcacaggtt ggcggtattt taaaatatac
    22561 ccgtccggcg tggtggcgtg gcgaaacgcc tgagacacat cactatttaa tgaaaaagcc
    22621 gggttattat ctttctgaag aagcctatat tgcccggttg cgtaaagaac ttaatttggc
    22681 gctttacagt cgttttccat taacgtggat tatggaagca gccgacgaca tctcctattg
    22741 tgtggcagac cttgaagatg cggtagagaa aagaatattt accgttgagc agctttatca
    22801 tcatttgcac gaagcgtggg gccagcatga gaaaggttcg ctcttttcgc tggtggttga
    22861 aaatgcctgg gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt
    22921 tatgtattta cgggtaaaca ccctaaataa actggtaccc tatgcggcac aacgatttat
    22981 tgataatctg cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag
    23041 cgaatgcagc gatcttctta agctatataa aaatgtcgct gtaaaacatg tgtttagcca
    23101 tccagatgtc gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat
    23161 ttatcgtcct ttattaagcc tgtcgttatc cgactttact gaactggtag aaaaagaacg
    23221 ggtgaaacgt ttccctattg aatcgcgctt attccacaaa ctctcgacgc gccatcggct
    23281 ggcctatgtc gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga
    23341 atattattac cgttgccgcc tgctgcagga ttatatcagc ggtatgaccg acctctatgc
    23401 gtgggatgaa taccgacgtc tgatggccgt agaacaataa ccaggctttt gtaaagacga
    23461 acaataaatt tttacctttt gcagaaactt tagttcggaa cttcgcgcta taaaacgaat
    23521 ctgaagaaca cagcaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac
    23581 cacattagca ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc
    23641 aacggcggct gagacttctt cagcaacgac agcccagcag atgccaagcc ttgcaccgat
    23701 gctcgaaaag gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa
    23761 tacgccgcgt atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca
    23821 ggaaggttct ccgttccaga gctctccgtt ctgccagggt ggccagggcg gtaatggtgg
    23881 cggccagcaa cagaaattca tggcgctggg ttccggcgtc atcattgatg ccgataaagg
    23941 ctatgtcgtc accaacaacc acgttgttga taacgcgacg gtgattaaag tccagctgag
    24001 cgatggccgt aagttcgacg cgaagatggt cggcaaagat ccgcgctctg atatcgcgct
    24061 gatccaaatc cagaacccga aaaacctgac cgcaattaag atggcggatt ctgatgcact
    24121 gcgcgtgggt gattacaccg tagcgattgg taacccgttt ggtctgggcg agacggtaac
    24181 ttccgggatt gtctctgcgc tggggcgtag cggcctgaat gctgaaaact acgaaaactt
    24241 catccagacc gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg ttaacctgaa
    24301 cggcgaactg atcggtatca acaccgcgat cctcgcaccg gacggcggca acatcggtat
    24361 cggttttgca atcccgagca acatggtgaa aaacctgacc tcgcagatgg tggaatacgg
    24421 ccaggtgaaa cgcggtgagc tgggtattat ggggactgag ctgaactccg aactggcgaa
    24481 agcgatgaaa gttgacgccc agcgcggtgc tttcgtaagc caggttctgc ctaattcctc
    24541 cgctgcaaaa gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccgat
    24601 cagcagcttt gccgcactgc gtgctcaggt gggtactatg ccggtgggca gcaaactgac
    24661 cctgggctta ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc agcagagcag
    24721 ccagaatcag gttgattcca gctccatctt caacggcatt gaaggcgctg agatgagcaa
    24781 caaaggcaaa gatcagggcg tggtagtgaa caacgtgaaa acgggcactc cggctgcgca
    24841 gatcggcctg aagaaaggtg atgtgattat tggcgcgaac cagcaggcag tgaaaaacat
    24901 cgctgaactg cgtaaagttc tcgacagcaa accgtctgtg ctggcactga acattcagcg
    24961 cggcgacagc accatctacc tgttaatgca gtaatctcac tcaacccctt cctgaaaacg
    25021 ggaaggggtt ctccttacaa tctgtgaact tcaccacaac tccatacatc ttcatcatcc
    25081 tttaggcatt tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataacg
    25141 gaggacttat ggctggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta
    25201 ccatgcgcat catcgatacc aatatcaacg taatggatgc ccgtgggcga attatcggca
    25261 gcggcgatcg tgagcgtatt ggtgaattgc acgaaggtgc attgctggta ctttcacagg
    25321 gacgagtcgt cgatatcgat gacgcggtgg cacgtcatct gcacggtgtg cggcaaggga
    25381 ttaatctacc gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac
    25441 cagagaatct gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac
    25501 agtcgcggtt gatgcacttg ttggcgcagg atagccgttt gcgggaggaa ctggtgatga
    25561 acctgattca ggcagaggag aatactcccg cacttactga atgggcgcaa cggctgggga
    25621 tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggtg
    25681 tggacagcgc aatggcggag ttacaacaac tgcaaaacgc gctgactacg cccgagcgta
    25741 ataatctggt ggcgattgtc tcgctaaccg aaatggtggt gttgaaaccg gcgttgaact
    25801 cttttgggcg ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca
    25861 tgaaagagta cggccggctg cgttttcgcg tttcactggg caactatttt accggtcctg
    25921 gcagtattgc ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga
    25981 tgccagaaag tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt
    26041 tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg
    26101 acaataacgg cttgctgcga cgaacgctgg cggcgtggtt tcgtcacaat gtgcaaccgc
    26161 tggcaacgtc aaaggcgttg tttatttatc gtaataccct ggagtatcgg cttaatcgta
    26221 tatcggaact gaccgggctt gatttgggca attttgatga caggttgctg ctgtatgtgg
    26281 cgttacaact ggatgaagag cggtaggtta tgcgttaagg ttgaggcggc gctgtctcat
    26341 tcccctcagc ccgaagggga gaggggaatg cccgaattaa ccgtcaattc gggattattt
    26401 acgcgttagt ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc
    26461 caggtgacgt aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg
    26521 gcagatttga tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc
    26581 tttataaccc tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa
    26641 cttaacgctc ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc
    26701 gttgttggct tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag
    26761 acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt
    26821 tagcatttgg cctagcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga
    26881 cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggtgat aaaattcacg
    26941 ttctacacca gttccagcat cgttacttcc gggcggcagt tcagacgcaa accatacaaa
    27001 ctgcccacgc cacgggttgt gtagatgtgt cgttcgccaa aggcatttaa tccggcgacg
    27061 taacgtttat cttcgacagg ggcaaaaggt tcaccaacca acggtacgcg cagttgcccg
    27121 ccgtgagtat ggccgcacag catcagatcc cagggttcat cacgcatgac ttctttgctg
    27181 tcgggattat gtgccagcat cagtcttggc agatttgctt cgctggcggg aggtggtttg
    27241 cattgtccgg cccataagtc accggtgcct accagttcga attgcctgtt cggcgtggcg
    27301 atcaccgtag cctggttaaa caacaccgtg atgcccactg atttcaacgt ctcgccaatt
    27361 aagtgatttt tttctgtacc aacagggcga tcgtggttgc cgaagcaggc aaacgtcggc
    27421 gcacattcgg caagggggga gaggacatca ctaaacgccg aaaaattcag cggcatatca
    27481 aataatacgt aatcgccgcc cagtaatatc aaatcgggct tttgttctat gccaagagca
    27541 atcgcgtcag aaatcaggct taaaggaaca aaacgagagt aatggagatc ggccagaaaa
    27601 agaattttga atggtgctgc gttgtcttta aaaaaggcga ggcggtgacg gattaattca
    27661 aaccagcctg gctcccagta atgcatataa ccgaaacctg agctcgtggc tatcgtcgcg
    27721 gcagtagcct gcaacaatcg gcggcgtgaa atcatcgctc atccctgcaa aaaaaatcgg
    27781 gcagcgtcgt gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag ttcgttaatg
    27841 ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg
    27901 tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc cggaacgtga
    27961 ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg
    28021 cccatggaaa tgacggaacc ttcttcgaca atcacccctt caactacttc agagcgtgcg
    28081 ccaatgaagc agttatcttc aatgatggtt gggttagcct gcagcggttc cagcacgccg
    28141 ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg
    28201 gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt gacgtaagac
    28261 ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc tggtggcaca
    28321 acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt cattggcact
    28381 ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag
    28441 agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct
    28501 acacgcagtg caccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg
    28561 gtgtctgcgt tggttggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc
    28621 tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt
    28681 ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagcgca
    28741 cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt
    28801 cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactct cgccagcagt
    28861 cctggctggt cgagcgcgat aagctcaagg aacgatttgc ggtcggtatg ggtcggcaaa
    28921 aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg acggggctgc
    28981 ggtggctgcc agctactttg cgtcagtact tgctccagac caaaccgaat aacctcatga
    29041 cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc catcgccata
    29101 ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg
    29161 gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct
    29221 ccacgcgtag cctgcgggct aagcaatacc agtggtttgc ttaaatcatg ctgtaataaa
    29281 tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag
    29341 cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt
    29401 gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt
    29461 cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc
    29521 cacagcgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag atagcgcaga
    29581 cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg
    29641 cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc aaccagctgt
    29701 gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg agcaccgaga
    29761 atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc
    29821 agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt
    29881 tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg
    29941 taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg cggcatatac
    30001 gcgccgagca cgctatggcg atgcattggc aatagcccgc gccgcaccgc tccggggtga
    30061 cgcagaatgc tcaaaaacag ttttcgtgct tccggaatat tacacagcgg ttgttgcaga
    30121 tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt gatcgcactg
    30181 ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt
    30241 tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgat tggacgtggt
    30301 ttttcgtcgg caggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg
    30361 agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc gaccggttcg
    30421 ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc gaataacagg
    30481 cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca cagtatatgc
    30541 agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc aaccatttca
    30601 tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg gatatcgcgc
    30661 aagccgccag ggctactttt gatgtctggt tcaagattgt agctggtgcc gtggtaacgc
    30721 tgatggcgct ggttctgttc ttcaactttc gccgcgtaga acttgtcgga aggccagaat
    30781 ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc aattaataag
    30841 cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccag catgcactct
    30901 tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc
    30961 tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg
    31021 atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcgac caatgccagg
    31081 tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtcgataaac
    31141 tcggtgcgcg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc
    31201 caacgctgga aagtatcgat atgggctttt atcccaccga cggttaattc atcacggggc
    31261 cagacgcatg gattttgcgg ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt
    31321 tctggaaggg tattcattgt gcgccactca taagattaaa ttatcgcatt aaaaaagccg
    31381 gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt
    31441 gcgtagcgtc agaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc
    31501 agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt
    31561 tttacccgcg ttgaccattg gctcgatggt gaacgtcatc ccaggtttca gtacgacgtt
    31621 ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc gaccaatacc
    31681 gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaact tctgaatcgc
    31741 cgcaccgatt tcgcgcaggt taatgcctgg tttgaccatg cgcagcgcca ggtacaggct
    31801 ttcttgcgtg atgcggcaca gacgttcgcc catgatggtc ggcttaccga cgataaacat
    31861 tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc
    31921 gccatctttc agcagcttcg catcgtccgg gataccgtgg cacaccactt cattaataga
    31981 gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg cgtgttgttc
    32041 attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac
    32101 atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc
    32161 gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggtgata
    32221 ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt
    32281 ctgaatttcg ccaaacgtgc cactgaacgt tttctataat agaaaattca acgtctgatg
    32341 ctgtacacag cgccaacaat tattggtgtc catgacgtat ttgtggtata aagcgcgccg
    32401 gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa
    32461 caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac
    32521 gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg
    32581 cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat
    32641 gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt
    32701 accgatgttc aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat
    32761 ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg
    32821 cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt
    32881 tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga
    32941 aaagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag
    33001 cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga
    33061 ccacgaacac attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt
    33121 tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat
    33181 ccgtgctgtg accctgtacc tgggcgctgt agctgcaacc gtacgtgaag gccgttctca
    33241 ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcttgataac
    33301 tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc gggagcttac
    33361 atcagtaagt gaccgggatg agcgagcgaa gataacgcat ctgcggcgcg aaatatgaag
    33421 ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc
    33481 ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt
    33541 agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc
    33601 atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa
    33661 aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac
    33721 ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag
    33781 actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca
    33841 gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt
    33901 gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc tgcgctggaa
    33961 ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa
    34021 ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa
    34081 ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg
    34141 gatatagcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc
    34201 atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc
    34261 aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt catccgcttc
    34321 gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt tgctgcgatg
    34381 tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat
    34441 cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag
    34501 tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc aatgcaaaac
    34561 ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc actgaaggct
    34621 tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa ctggttgaac
    34681 tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc gctggtctgg
    34741 cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg accgtaatga
    34801 acggcctggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg
    34861 ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc agcctgttgc
    34921 gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt accaccgact
    34981 cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag
    35041 ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg tacgagcaac
    35101 tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg gccttcacgc
    35161 tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc
    35221 gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc ccgtgatgga
    35281 taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt ataaggcgtt
    35341 attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac aaaagtacgt
    35401 gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa taatccagac
    35461 gattacccgt aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg
    35521 tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag
    35581 atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac
    35641 gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtagaatat tacggcacgc
    35701 cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca
    35761 acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg
    35821 gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag
    35881 aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag
    35941 tacgtaacgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga
    36001 tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa
    36061 tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc
    36121 ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt
    36181 ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc
    36241 tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt
    36301 gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc
    36361 cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa
    36421 cagggtagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt
    36481 gaggatgttg atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt
    36541 gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc
    36601 ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc
    36661 gaacataacg ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct
    36721 gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt
    36781 gagacgccat tgcgcgattt ggcaacaatg acgccggatc aagcctgccg tcatccgaac
    36841 tggtcgatgg ggcgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa
    36901 tacattgaag cgcgttggct atttaacgcc agcgccagcc agatggaagt gctgattcac
    36961 ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg
    37021 ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac
    37081 tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat
    37141 tatgatcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg tcaggcagcg
    37201 acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc
    37261 cgctttacgg atatcgctgc gttgaattta tcggtactgg aaaaaatgga tatgcgcgaa
    37321 ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa
    37381 gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtctcg ctatttgtta
    37441 gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg
    37501 tcaggtatgc cgtggttttg cacggctttt tttgtatagg cttcagtatt cctgagtacc
    37561 gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct gctactcaac tacttagcga
    37621 aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg
    37681 ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg
    37741 ccgggctgtc tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag
    37801 tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg
    37861 ggcgctcgat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg
    37921 cgataccagt cgctttaact cgcgtttgca agaatgtatt cgtaaatctg aagcgctaac
    37981 agccgggaat accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat
    38041 agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca
    38101 gatagatgaa gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt
    38161 agtaattagg actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta
    38221 tgccgaactt tactttatag atgttctctg gcccgatttc gatgaacaag actttgaagg
    38281 ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga
    38341 aacagcctga tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat
    38401 acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca ttgtaacgct
    38461 ggtggtctgc atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc
    38521 gcagcgagta tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt
    38581 gccggaatat caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgct
    38641 gggttggtgg attgtctcgc tattgctggt gctgttttac ccaggttccg cagcaatctg
    38701 gcgtaactct aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg
    38761 gggcatgctg gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg
    38821 gctgctctat gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa
    38881 attgtttggt aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt
    38941 tataggtgga ctcgctactg cagcggtaat ctcatggggt tatggcatgt gggcgaatct
    39001 cgacgtcgct cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct
    39061 cggcgatctg accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt
    39121 aattccagga cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt
    39181 ctttgcttgc ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt
    39241 tttctctggg atttggcttc gttcatcgtt gcactgggtg tacttatcac tgtgcatgaa
    39301 tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg
    39361 tttggtaagg cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatcgccctg
    39421 atcccgctgg gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa
    39481 ctccgccacc atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca
    39541 ggtccggttg caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt
    39601 gtgcctggcg tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca
    39661 caaattgcac caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat
    39721 gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg
    39781 ccatttggca gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag
    39841 cctgataaag aagatccggt atcttcgctg gggattcgtc ctcgtgggct gcaaattgaa
    39901 cctgtactgg aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc
    39961 gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt
    40021 gtccgggata acccggggaa atccttagcg ttagaaatcg aaaggcaggg aagtcccttg
    40081 tctttgacat taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt
    40141 attgagccga aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg
    40201 ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt
    40261 atgctgggaa aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct
    40321 atcgccaagg gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt
    40381 gcgcttatta gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg
    40441 gcgcatctgc tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt
    40501 caagactttt gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt
    40561 ttcaatgatt tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga
    40621 tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag
    40681 ggttcgtagt gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggccc
    40741 tcctcagtat gccggtgcgt acaggcgaca cggttaatga tgaagatatc agtaatacca
    40801 ttcgcgctct gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata
    40861 cccttctggt tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca
    40921 aatcggtgaa agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg
    40981 aatccctcga tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca
    41041 gcgtcggtaa atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg
    41101 ttgacctaaa actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg
    41161 ttggtaacca tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag
    41221 tgccgtggtg gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc
    41281 ttgaaaccct gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta
    41341 cccaggtcag tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag
    41401 gcgatcagta caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg
    41461 aaattgagca gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca
    41521 agatggaaga tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac
    41581 agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg
    41641 cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg
    41701 ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg
    41761 atcagggtaa ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc
    41821 aacgtgttcc gggtagcccg gaccaagtag atgtagtcta caaggtgaaa gaacgtaaca
    41881 ccggtagctt caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg
    41941 gcgtgcaaca ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa
    42001 acgattacca gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg
    42061 taagcctcgg tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg
    42121 actataccaa caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata
    42181 actcgctgcg tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg
    42241 ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata
    42301 acagcttcaa aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc
    42361 gtggttactt cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg
    42421 gatcggataa cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg
    42481 acgatcacaa atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg
    42541 gcaaagagat gccgttctac gagaacttct atgccggtgg ttccagcacc gtccgtggct
    42601 tccagtccaa taccattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg
    42661 atccggacta tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg
    42721 atgatgctgt aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc
    42781 cgtttattag cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta
    42841 ccgtttggga tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc
    42901 caagcaatat ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt
    42961 tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc
    43021 agtttaacat cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag
    43081 cgatgacttt aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg
    43141 tgcaaatggg atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt
    43201 ttagcactgg caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc
    43261 ctgttccagc aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa
    43321 ggccgtgcca gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg
    43381 cagtccatga aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc
    43441 cagacttttg ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa
    43501 gaacgcggca aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag
    43561 gatatcgatc tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac
    43621 atcactgccg acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat
    43681 ttagcgcagc agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt
    43741 gcgtccatgc aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt
    43801 gagcatttag gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc
    43861 gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa
    43921 attttagata ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg
    43981 acggcgaagc tggggaacaa tgtatcgatt ggcgctaacg cggtgattga atccggcgtt
    44041 gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc
    44101 ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag
    44161 aattgcctga tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat
    44221 cgtggtaact gggttaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag
    44281 atcggtgcct gcactaccat cgatcgcggc gcactggatg acactgttat tggcaatggc
    44341 gtgatcattg ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg
    44401 gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc
    44461 ggagccagcg taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg
    44521 ggtatggtga tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa
    44581 cccaacaaag tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag
    44641 cgtctgaaat cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg
    44701 ccaaacttta cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt
    44761 tcttatattt tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag
    44821 agattttaga acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt
    44881 ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc
    44941 agggccattt ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac
    45001 aggcaacagg tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact
    45061 acttcgctgg tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga
    45121 tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc
    45181 tggtcgatgg taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct
    45241 gatacgtgat tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt
    45301 caattggcgc gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcggaattg
    45361 gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg
    45421 ataatgagat ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg
    45481 gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc
    45541 atcgtggcac agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga
    45601 tcaacgcgca cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg
    45661 caacgctggc gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag
    45721 tccatcagtt ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc
    45781 aggacgtccc tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata
    45841 tcgaagggct gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt
    45901 ataagctgat ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac
    45961 tggcggaaac atatccggaa gtgaaagcct ttaccgattt cttttcacgc tcaacgcgcg
    46021 gtctgattcg ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac
    46081 ctccggcgat atcctggggg ccggtttaat ccgcgctctg aaagaacgtg tgcccaacgc
    46141 ccgctttgtt ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cctggtacga
    46201 aatggaagaa ctggcggtga tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact
    46261 gcatattcgt gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg
    46321 tattgatgcg cctgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa
    46381 aaccattcat tacgtcagtc cgtcagtctg ggcgtggcga cagaaacgtg ttttcaaaat
    46441 aggcagagcc accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa
    46501 atacaacgta ccgtgccgct ttatcggtca taccatggct gatgccatgc cattagatcc
    46561 agataaaaat gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt
    46621 gctaccgggg agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc
    46681 ccagcttttg cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa
    46741 acgccgcgag cagtttgaac gcatcaaagc tgaagtcgcg cgagactttt cagttcattt
    46801 gctggatggg atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg
    46861 tacggcagcc ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa
    46921 gccttttacc ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa
    46981 tctgctggcg ggcagagagt tagtcaaaga gttattgcag gaagagtgtg agccgcaaaa
    47041 actggctgcg gcgctgttac cgctgttggc gaacgggaaa atcagccacg cgatgcacga
    47101 taccttccgt gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc
    47161 cgttctggag ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt
    47221 gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt
    47281 gacccggcgc gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt
    47341 ctggcgctct gtgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa
    47401 ccccacgaaa tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc
    47461 gtcgctgggc tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa
    47521 ttaccgatgc ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc
    47581 gcgtctatcc tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc
    47641 ccgcaatatg gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg
    47701 gctgaacacg gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg
    47761 ggacttgcgt cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa
    47821 ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa
    47881 actgcaccgt tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat
    47941 ttcaccaacc tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag
    48001 cctatcgtcg gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac
    48061 ctgacggtac tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa
    48121 gcgtatcagc gcgggtacgg tgccgctggg ccgatcatcg atcgcgactg gcttatcgaa
    48181 ttaaatgaag ggttgatcct tctttccggc gggcgcatgg gcgacgtcgg acgcagtctt
    48241 ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg
    48301 gatcgctatt ttctcgagct gatccgcacc ggcaggccgg atgaagaaag ctatctgcac
    48361 gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgactaa cgatgtgcgc
    48421 tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt
    48481 accctcgacg atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa
    48541 gaggagatgt gtgagctgtt tgccgacatc cccgaagccc ttgccaacac cgttgagatc
    48601 gccaaacgct gtaacgtaac tgtgcgtctt ggtgaatact tcctgccgca gttcccgacc
    48661 ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt
    48721 ctggcctttt tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa
    48781 cgtctggaga ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc
    48841 gttatggaat ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc
    48901 tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa
    48961 tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac
    49021 gttgacttct gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt
    49081 cgtgatgcgg tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc
    49141 gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc
    49201 ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa
    49261 atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg
    49321 gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc
    49381 gattttgcgc cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa
    49441 agcgacgttg aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc
    49501 atcatcaact gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg
    49561 ctggatatcg ccgcgatccc gctggatgac aagaaaagct tcgacatgct gcaacgctcg
    49621 gaaaccacgg cggtattcca gcttgaatcg cgcggcatga aggatctgat caagcgtctg
    49681 caacctgact gcttcgaaga tatgatcgca ctggtggcgc tgttccgccc aggtccgttg
    49741 caatcaggga tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga aatctcctat
    49801 ccggacgtac agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc
    49861 atcctgtatc aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt
    49921 ggcgcggata tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag
    49981 cgttctgtat ttgctgaagg tgcagaaaag aacggagtca acgccgaact ggcgatgaaa
    50041 atcttcgacc tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc
    50101 tatgctttgg tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg
    50161 gcggcggtaa tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag
    50221 tgctggcgga tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc
    50281 cacgtcaacg acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa
    50341 ggtccgattg aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt
    50401 gatctctgcg cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc
    50461 atgtccgggg cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt
    50521 gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg
    50581 ttcggcgtgc tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg
    50641 tggccggagc aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga
    50701 caccctatca accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa
    50761 gacatgcacc cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg
    50821 cgggttatgg tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc
    50881 gggcggctgg aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa
    50941 aaagaccgca tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt
    51001 aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctagcggg
    51061 cttgctatct cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag
    51121 tctctggaac cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat
    51181 gcacgcgcgc ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta
    51241 aacgatctcc gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg
    51301 aatactatga gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa
    51361 atcgattctc tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa
    51421 gaagtgcatc gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc
    51481 ggtgcatggc agattgcgca actggcacgc catccacagc gtccttatac cctggattac
    51541 gttcgcctgg catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat
    51601 aaagctatcg tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat
    51661 caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa
    51721 ggttaccgca aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc
    51781 acctttatcg acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct
    51841 gaagccattg cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg
    51901 gttatcggtg aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat
    51961 atgctgcaat acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg
    52021 aagagcgccg acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg
    52081 aaagagctga aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac
    52141 ccggaagcga tggcggcatc gttgaaagcg caactgctgg cggatctggc cgatctcgat
    52201 gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac
    52261 gcgtaattcg caaaagttct gaaaaagggt cacttcggtg gccctttttt atcgccacgg
    52321 tttgagcagg ctatgattaa ggaaggattt tccaggagga acacatgaac atcattgcca
    52381 ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg gagtcggcgc
    52441 tggtggcgca aggctttcag attatctggc cacaaaacag tgttgatttg ctgaaattta
    52501 tcgaacataa ccctcgaatt tgcggcgtga tttttgactg ggatgagtac agtctcgatt
    52561 tatgcagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc atcaacaccc
    52621 actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt tttgaatatg
    52681 cgctggggca ggcggaagat atcgccattc gtatgcgtca gtacaccaac gaatatcttg
    52741 ataacattac gccgccgttc acgaaagcct tgtttaccta cgttaaagag cggaagtaca
    52801 ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg gttggctgcc
    52861 tgttttatga ttttttcggc gggaataccc tcaaggctga tgtctctatt tcggtcaccg
    52921 agcttggttc gttgctcgac cacaccgggc cacacctgga agcggaagag tacatcgcgc
    52981 ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg tcgaacaaaa
    53041 ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc aattgtcata
    53101 aatcgctggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg aaaccgacgc
    53161 gtaatgcgtt ggggattctt ggtgggatcc cgcaccgtga atttactcgc gacagcatcg
    53221 aagagaaagt cgctgccacc acgcaggcac aatggccggt tcatgcggtg attaccaact
    53281 ccacctatga tggcttgctc tacaacaccg actggatcaa acagacgctg gatgtcccgt
    53341 cgatccactt cgattccgcc tgggtgccgt acacccattt tcatccgatc taccagggga
    53401 aaagtgatct tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttcg
    53461 ctgatccaca ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat
    53521 accaccacct cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgacgctg
    53581 cgtggtaatc cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc
    53641 aaagaggtcc agcggttgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg
    53701 ccgcaggtgg atgaagccga atgctggccc gttgcgccag gcgaacagtg gcacggcttt
    53761 aacgatgcgg atgccgatca tatgtttctc gatccggtta aagtcactat tttgataccg
    53821 gggatggacg aacagggcaa tatgagcgag gaggggatcc tggcggcgct ggtggcaaaa
    53881 ttcctcgacg aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc
    53941 tttagtattg gtatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt
    54001 aaacgctctt acgatctcaa cctgcggatc aaaaatatgc tacccgatct ctatgctgaa
    54061 gatcccgatt tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg
    54121 attcgtaaac acgatcttcc cggtttgatg ttgtgggcat tcgatacttt gccggaaatt
    54181 atcatgacgc cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg
    54241 ctggaacaac tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta
    54301 ccgctgttga tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta
    54361 ctgatgcttt gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg
    54421 aaacaggacg aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc
    54481 agagcggctt acgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt
    54541 taaaacaggt tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct
    54601 actgcgatat tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt
    54661 ggaaagggga tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc
    54721 cgccggagcg ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg
    54781 atgacatcga tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc
    54841 gtgtcgatcc atacacgcaa aaacgcttca ccttctttaa cgatccggac gggctgccgt
    54901 tggaactgta tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca
    54961 ttgctactgt aaaatcgcac catcatgaca ctcacgctca atagacaact tctcacctca
    55021 cgccagattt tggtcgcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg
    55081 gtgcagtggc ggacggaaaa tccgggcgtc actctgcgcg ctattcatgt gcatcacggt
    55141 ttaagtgcca atgccgatgc ctgggttacg cattgcgaaa acgtctgcca acagtggcag
    55201 gtgccgctgg tggtcgaacg cgtacaactt gcgcaagagg ggctggggat tgaggcccag
    55261 gcgcggcagg cgcgttatca ggcatttgcc cgtaccctat tgcccggcga agtgctggtc
    55321 accgcgcaac atctcgacga tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc
    55381 ggccctgccg ggctttcggc tatggcggaa gtttcggagt ttgccggaac acggcttatc
    55441 cgcccgttgc ttgcccgcac gcggggggaa ctggcgcagt gggcattggc gcatggttta
    55501 cgctggattg aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg
    55561 cgcgtagtgc cgttattgca gcagcgttgg ccgcattttg cggaagcaac ggcccgcagc
    55621 gccgcacttt gtgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca
    55681 cactgccaaa cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc
    55741 cgccgcgcgg cgattatccg tcgctggctg gcagggcaga atgcaccaat gccttcccgc
    55801 gacgcgctgg tgcggatctg gcaggaagtg gcgctggcgc gggaagatgc ctccccctgt
    55861 ttacgcttgg gcgcgtttga aatccgccgc tatcagtcgc aactgtggtg gattaaatcc
    55921 gtcaccgggc aaagtgaaac cattgtgccg tggcagacgt ggctacaacc gctggaatta
    55981 ccggcggggc tgggaagcgt acagcttact gcgggaggcg atattcgccc tccgcgtgca
    56041 gacgaagcgg tcagcgtgcg tttcaaatcg gcaggattgc tgcatattgt cgggcgcaac
    56101 ggcggacgta agctgaagaa aatctggcaa gagttaggcg tgccgccgtg gctacgtgac
    56161 accacgccac tgctgtttta tggcgaaacg ctgattgcgg cggcaggggt atttgtgacg
    56221 caagaaggtg tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt
    56281 taagtgaaag ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggactc
    56341 gctcaccacc accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgtag
    56401 ctcacgggtt tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa
    56461 atcgctggct tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg
    56521 atgctggcag gcgagctcaa gattatcgta atcatcacaa ttgattggtt gatacgtatc
    56581 attcattaac ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga
    56641 cagcctcaga aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca
    56701 gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta
    56761 actcacaata tttttccaca tgccctcctg tcagcacact gacttaaccg tggatgcaag
    56821 tctaagccta cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca
    56881 atatcagcgc cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt
    56941 tcttatggcg cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg
    57001 caaccagttt ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg
    57061 tatgatgctg cgcctgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg
    57121 tggtttgtgt gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga
    57181 tttgtggatt gagctggggc tgccggatga gcggcggatt aagaaagcct gcacccaggc
    57241 cgcagaagtg gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa
    57301 tcagagcaaa tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact
    57361 ggcgaaagta agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg
    57421 cgtgatctgg ttatcggacg ataagaataa tctggaagtt aacttaaccg cctggcaaca
    57481 accttcatga ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc
    57541 gccattcgtg cgcagggcgc gggcgggcag catgttaata agacctcaac ggccattcat
    57601 ctgcgttttg acattcgggc gtccagcctg ccagagtatt acaaagagcg tctgctcgcc
    57661 gccagccatc atttgatcag cagtgatggc gtgattgtca ttaaggcaca ggaataccgc
    57721 agtcaggaac tgaaccgcga agccgcgcta gcccggctgg tggcagtgat taaagattta
    57781 acaacagaac aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg
    57841 ctggcatcga aagcacaaaa atcaagcgcg aaggcgatgc gcggcaaagt acgcagcggt
    57901 cgggaataaa aagaaggaat agatggtgaa aaaagcgata gtgacagcga tggctgtaat
    57961 cagcctcttt actctgatgg gatgtaataa ccgggccgaa gtcgatacgc tttctccagc
    58021 acaggctgcc gaactgaaac cgatgccgca aagttggcgc ggcgtgctgc cgtgtgctga
    58081 ttgtgaagga atcgaaacct ctctgttcct cgaaaaagac ggaacatggg tgatgaatga
    58141 gcgttatctc ggtgctcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg
    58201 aaccgctgac aagctggtat taaccgacag caaaggtgaa aagtcatatt atcgggcgaa
    58261 aggcgatgcg ctggagatgc tcgatcgtga aggcaatccg attgaatcgc agttcaacta
    58321 tacgctggaa ccggcacaat ccagtttacc tatgacgccg atgaccctgc gaggcatgta
    58381 tttttatatg gctgatgcgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt
    58441 agcgaataac gcagagctgg agcgtggcta cctggctgcg cgcggtcaca gtgaaaaacc
    58501 ggtgttactg tcagtagaag gtcactttac gcttgaggct aatccggata ctggtgcgcc
    58561 aaccaaagta ttagcgcccg atacggcggg taaattttac ccgaataagg attgcagtag
    58621 tttggggcag taaacctgct taaggcagaa acaaacgcaa aacaggccag aaggatatat
    58681 ttcaacattt tgaatttgca cgttttttgt aggccggata aggcgtttac gccgcatccg
    58741 gcaatggtgc tcaacgcctg atgcgacgct ggcgcgtctt atcatgccta caacccccct
    58801 cataccttaa ggcggataag gcatttactt tatcaccggg tcaaaccaac aaagcgaccc
    58861 gccttgatat aaatccctgg cttcaggtgc ccaacatact gtaactgcgt ttctggctta
    58921 aacgcagaga catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat
    58981 tgcgccagtg taagtggatg tccggctttg gcaaatgcat cagtgacaaa ttccgaacaa
    59041 aaccacgact ttttgtctcc ttcgcccaca ctgcttaact gcgctttcgc caggccgctg
    59101 acgcactgct ggcgaaaatc ctcggagaac tgattcagtg agcacatctg gcgagtcacc
    59161 ataaagggaa taaattcgac aatgccgcga tagttataac cgctatcttt gattttattg
    59221 gcaaacgcgg tgatatctgt ggcttgttgc ggggtaagat ccgggactcg taagacgaaa
    59281 agcttatcac tatgcttcat cgctttttta agggaaacaa tctggacgcc agcgcctgtc
    59341 gcttctgcaa cgttattatc acccagaaag attgcaacgt gactcacaga ggaagtgctg
    59401 aagacgcgga ttccaaatga ggttacccca aggcttgagg agaacagcaa atcgccgggt
    59461 ttgagatcag gtgccgttat ttcttttatt gattgttcgg tgaaagagct ttgatgctgg
    59521 aatttgacag cccatgtttt tgcctcggca tctactgctg tggctgatgg ggctggctga
    59581 tatcaacggt acaagccgaa agtaaaagaa agctggggag aagcaggcgg cagtacgcct
    59641 ttggtttatc cattttatac aatccatgta aaaaagggcc ctgaaattca ggaccctttc
    59701 tggcatcagc ctttaatctg tttcaccaga tattcgacga tgtcaccagt cttaattaac
    59761 tgtttctcgc cgttgcgacg atatttatat tcgatatcgt cgttgtcgag gttacggtcg
    59821 cccagcacaa tagtgtgcgg aataccgatc agttccatat cagcaaacat cacgcccgga
    59881 cgctctttgc ggtcatccag cagcacttcg ataccttgtg cacgcagttc gctgtacagt
    59941 ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag aatcgccacc
    60001 tggaacggcg cgatagcgtc aggccatacg atgccgcgtt cgtcgtagtt ctgctcaatc
    60061 gccgcagcta ccacacgcgt taccccgata ccgtagcaac ccatcgtcag gatttggtta
    60121 cggccatctt caccctgtac ggaggctttc agtgcttcgg agtacttggt acccagctgg
    60181 aagatgtgac caacttcgat accacgtttg atcagcagcg taccctggcc atccgggctt
    60241 ggatcgccag ccaccacgtt acggatatct gcaacttccg gggtagcgac atcgcgatcc
    60301 cagttgatac cgaagtagtg tttaccatcg atgttagcac cagcagcgaa atcactcatc
    60361 gccgcaacgg tacggtcaat caccaccgga atcggcatgt ttaccggacc cagtgaaccc
    60421 ggaccggctt taaccacagc acgaatttct tcttcggtcg cgaaagtcag cgggcttgca
    60481 acctgcggca gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg caccagcagc
    60541 gcaaccagcg ggaagctgct gccttcaacc gctttaacca gcagagtctt aaccgttttc
    60601 tcaatcggca gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt cggcgtatca
    60661 accagcgtca tttcctgggt agcagcagcg cgcggttctt tcggcgcgat agcttctgcc
    60721 agttcaatgt tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc ttcaccgctc
    60781 tgcgccagca cctggaattc gtgagaggcg ctgccgccga tagaaccggt gtcggcttgc
    60841 acggcgcgga aatccagccc catgcggctg aagattttgc tgtaggccgc atacattgca
    60901 tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag agtaagcatc tttcatcagg
    60961 aattcgcggg aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa cttggtctgg
    61021 atctgataga agttcagcgg cagctgtttg taagagctaa gctcgttacg aatcaggtca
    61081 gtgataactt cttcatgagt tgggccgagt acgaacggac gctcgccacg gtcaacaaaa
    61141 cgcagcagtt ccggaccgta ctgttcccaa cgaccactct cttgccacaa atcggctggc
    61201 tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt tcatctcttc acgcacgatg
    61261 ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc aggtatataa cccggaggcc
    61321 agcttgcgga tcatcccggc gcgcagcatc agctgatggc tgatcacctc ggcgtcggca
    61381 ggtgtctcct tgagagtgga gagcaggtat tggctagtac gcatgttgtt acggttccag
    61441 ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa aaagtgattt agtttaccag
    61501 tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg cggttccagc gcaaagactt
    61561 caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc atgtagccag acggcatagg
    61621 ttttgcccgt ttcctcacct ttatgatagg ccgggcgcgg gtcctgcgcc agtacttcgc
    61681 ggataaacag cgttaactgc ggataacgct tctccagcgt caaaagctgc ttttcgacct
    61741 ctgcggtaaa actcaccgcc atctctgcag ctggcgcact ttgcgcatag ctggcactgg
    61801 catcgggaag cgattcggca aaggggagat acggtttgat atccactacc ggcgtaccat
    61861 cgaccagatc cagactgccg agcttcagaa tcacgctgtc tttatggcaa acaacctctt
    61921 tcagctctac cagcgacata ccaattgggt tagggcggaa agtagagcgt gttgcgaaaa
    61981 cccccattct ggcgttaccg ccgaggcgcg gtggacgcac agtcggacgc cagccgcctt
    62041 ccatcgtttg atgaaagacg aaaaggatcc ataaatggct gaacgcttcc aggccgcgaa
    62101 cggcgtcggc ctggttgtag ggagcaatga gatgcagttc gccgttggcg ctttttacca
    62161 gacctggctg gcgcggaacg gcgaattttt ctttataggg cgagcgaata acgcctattt
    62221 gctcgaactg gaaactgctc atttcgccgt aatgttaagc gcagagccga tacatacagc
    62281 ctgacgatag cagcctggcg taccgctggt gacttcgcag ctatgcagta ataccgcatt
    62341 ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc gcggttggaa tgctcggcgg
    62401 agagtcctga ttagaggcct ggcaagagtc gccactgact tcaccgagat cgcggaacgg
    62461 tttgccgact aattcttctg cattggtata aattcggacc ggcgtggcgc gcggcgcttt
    62521 cggttttgca ggctccgctt tcggctgggg tgcagtgctt tgaacgggtt cgacagggga
    62581 tctgcttaac atggaacagc cgcttagcat gagtgctact aaacagatcg gtaaagcacg
    62641 catagtattt cctcaatgta tgatcaaaac gtcaatattg aatcaggagc ttgtaaaaat
    62701 gacaagacgg gcaagtgccc atcctgaatg atattacaaa ttgtggaaac agcctaaaaa
    62761 ttaccagcct ttaacagcgc cgccgttaaa cactttgttt gctgcttcgt aaacttcatc
    62821 agactgataa gcctggacga atttcttcac gttctcggcg tctttgttat cttcacgcgt
    62881 tacgatcagg tttacgtacg gggagtcttt atcttcgaca aagataccgt ctttcgctgg
    62941 agtcaggcca atctggctgg cataggtggt attgataact gccagagcga tttgtgcgtc
    63001 gtccagagat cgaggcagct gcggtgcttc cagctcaaca attttcagat tttttgggtt
    63061 ctcaacaaca tcaagtacgg tcggcagcag gccaacgcca tctttcagtt tgatcaagcc
    63121 cactttttgc agcagcagca gtgaacgacc aaggttagtt ggatcgtttg gcacggcaac
    63181 ctgcgaacca tcctgcagtt catccagtga tttgattttc ttggagtaac cagcaatcgg
    63241 ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca cgatctttca gttgctgatc
    63301 aaggtacggt ttatgctgga aggcgttggc gtcgatatcg cctttgctca atgcttcgtt
    63361 tggcagaaca tagtcgttga aggttaccag ctcaacgtcc aggccatatt tgtctttcgc
    63421 aactttctgc gcgacttctg caacctgctg ttcggcacca acaatcacgc cgactttaat
    63481 gtggtttgga tctttttcat cctgaccgca gcctaccagt gccagtgatc caatcagggc
    63541 tcccactgcc gcaaaggttt tgaatttgaa cgccatacct tattccttct tcaattattt
    63601 atgttgtgtt gaacgttact tgcgagtgac agcccggacg atgcggtcgc ctgcgaactg
    63661 aattaaataa accagaatga ccagcaatac cagtaccgta ttcatcaccg tcgcgttata
    63721 gccgatgtag ccatactgat agccaatctg acctaaacca ccggcaccga cagcaccacc
    63781 catcgcggaa taaccgacta gggtaatcag ggtgatagtt gccgcattca ccagacccgg
    63841 caatgcttcc ggtaacagca ctttacggac gatctgcatc ggcgtggcac ccattgcacg
    63901 ggaagcttca attaacccgg ttgggatctc cagcagagcg ttctcgacca tacgggcaat
    63961 aaacggtgct gcaccaacgg ttaacggaac aatcgctgcc tgcaaaccaa tcgatgtacc
    64021 aacaataacg cgggtaaacg gaatcatcca taccagcaag ataatgaacg ggatagaacg
    64081 gaaaatgttc acaatcgcag aaacggtacg atacagcttc gcgttagcaa taatttgccc
    64141 cggacgcgtg acataaagca gaacgccaac cggcagacca atcacaaagc caaaaaaacc
    64201 ggatacgaag gtcattgcca gcgtttccca tacgccacga accagcagcc acatcatcgg
    64261 ctcagacata acccagtacc tctactttta catggtgttc ctgcagccag gcaatggcgg
    64321 cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat gatgccgaac ttaacgccac
    64381 cggcgtagtc catctgcgcg ctaataatgt tgttgttgac gttgaaacga cgcgcggttt
    64441 cagaaagcag tggggcatcg accgattgac cggtaaactc cagacgcagc atcggcacgc
    64501 agtcagtaaa tggctccgct tgcagacgtt cctggtaatc ttccgggata tccagatgca
    64561 gggtcgactg aataaacttc tgcgccagcg gcgttttcgg atgcgagaac acttcactta
    64621 ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc cacgcaatca caaatacgct
    64681 tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa acccagacgg cggttaatgt
    64741 ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc cagcgcgctg gtggcttcat
    64801 cacacagcaa tactttggga ttgctggcta atgcacgggc aatcgccaca cgttgtttct
    64861 gcccaccgga aagattcgac gggtagctat catgcttatc gccaagacca accaatgaca
    64921 gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt gttgtccagc tccagcggca
    64981 gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt aaaatgctgg aaaatcatac
    65041 caatctggcg gcgagctttg gtcaactcgg attctgacag cgtggtcagt tcctggccat
    65101 cgaccagcac gctaccctcg gttgggcgct ccagcaggtt tacacaacgt ataagcgtac
    65161 tcttacccgc gcctgaggca ccgataacgc cataaatttg tccagctggc acatgcaggc
    65221 tgacgttgtt caacgcctgg atggtgcggg tgccctggtg gaacactttg gtgatattcg
    65281 aaagttttat cattgattat ttattatcgt cattaagtta gtcgtggcat ctcgaatgcc
    65341 tgaaacgggc aacgccgtca atgaaatgga tgttaaggca tccagacgtc taaatcaatc
    65401 aggtttatgc gaagagcact ttcttgcagg tcgaaacatg cgatactagc gtcacatgcc
    65461 ttattaagga gctataaaag gtggcaaaga gcgtacccgc aatttttctt gaccgtgatg
    65521 gcaccattaa tgtcgatcac ggctatgtcc atgagatcga caactttgaa tttatcgacg
    65581 gtgttattga cgccatgcgc gagctaaaaa aaatgggctt tgtgctggtg gtagtaacca
    65641 accagtctgg cattgctcgc ggtaaattta ccgaagcaca gtttgaaacg ctgaccgagt
    65701 ggatggactg gtcgctggcg gaccgagatg tcgatctgga tggtatctat tattgcccgc
    65761 atcatccgca gggtagtgtt gaagagtttc gccaggtctg cgactgccgt aaaccacatc
    65821 cggggatgct tttgtcagca cgcgattatt tgcatattga tatggccgct tcttatatgg
    65881 tgggcgataa attagaagat atgcaggcag cggctgcggc gaacgtggga acaaaagtgc
    65941 tggtgcgtac gggtaaacct attacgcctg aagcagaaaa cgcggcggat tgggtgttaa
    66001 atagcctggc agacctgccg caagcgataa aaaagcagca aaaaccggca taatgattaa
    66061 aagatgagcg attgaaataa aaatgcattt ttccacttgt cttcctgaga cgactcccta
    66121 taatgcgcct ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt
    66181 gaagagaaaa atcctgaaat tcag
//
LOCUS       DAJTOR010000004        64376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTOR010000004 DAJTOR010000000
VERSION     DAJTOR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.004"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..346)
                     /locus_tag="OO249_000239"
     CDS             complement(<1..346)
                     /locus_tag="OO249_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5634664.1"
                     /translation="MFYSPSLNIFVNPALKDDYINANSWPDDALAVSDDVYNEFAINT
                     PPDDKIRVAGKNGLPTWALIPPPSYEELIQQAESERQLLLNQANEYMNSKQWPGKAAI
                     GRLKGEELAQYNL"
     gene            complement(348..734)
                     /locus_tag="OO249_000240"
     CDS             complement(348..734)
                     /locus_tag="OO249_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5634665.1"
                     /translation="MPVSHYLCIFLNVGLGELSLAGTASGVIGLNGYVTIPLIISGSR
                     RTLIIQWGQARFGGSGGEDAGYLNDFPFAFPSACYGMIVSHVGHTPSGAGILSASAIT
                     SNQFRGFSSIATAANAVLGRYIAIGV"
     gene            765..1331
                     /locus_tag="OO249_000241"
     CDS             765..1331
                     /locus_tag="OO249_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5634666.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            1474..2646
                     /locus_tag="OO249_000242"
     CDS             1474..2646
                     /locus_tag="OO249_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HCR5634667.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDITTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            2656..3171
                     /locus_tag="OO249_000243"
     CDS             2656..3171
                     /locus_tag="OO249_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCR5634668.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            3226..3528
                     /locus_tag="OO249_000244"
     CDS             3226..3528
                     /locus_tag="OO249_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5634669.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            3543..3662
                     /locus_tag="OO249_000245"
     CDS             3543..3662
                     /locus_tag="OO249_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR5634670.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            3655..6732
                     /locus_tag="OO249_000246"
     CDS             3655..6732
                     /locus_tag="OO249_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5634671.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISANDGASATARITRETMRYNRQLSEQEAR
                     LRRVGEQQRKMHAARGAYARRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQGAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTATVTRTSNALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAATGTVLRMLASGPLAMLRVALYAVSGLLGALLSLIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAATPAELPPLHPLALPAKGGEAMVSRAATVPPV
                     QRIEAPMQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            6729..7214
                     /locus_tag="OO249_000247"
     CDS             6729..7214
                     /locus_tag="OO249_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5634672.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     S"
     gene            7211..8311
                     /locus_tag="OO249_000248"
     CDS             7211..8311
                     /locus_tag="OO249_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCR5634673.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPLDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            8402..8620
                     /locus_tag="OO249_000249"
     CDS             8402..8620
                     /locus_tag="OO249_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5634674.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(8855..10540)
                     /locus_tag="OO249_000250"
     CDS             complement(8855..10540)
                     /locus_tag="OO249_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5634675.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            10810..11187
                     /locus_tag="OO249_000251"
     CDS             10810..11187
                     /locus_tag="OO249_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5634676.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(11217..11474)
                     /gene="grxA"
                     /locus_tag="OO249_000252"
     CDS             complement(11217..11474)
                     /gene="grxA"
                     /locus_tag="OO249_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5634677.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            11634..11921
                     /locus_tag="OO249_000253"
     CDS             11634..11921
                     /locus_tag="OO249_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5634678.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            11905..12627
                     /gene="nfsA"
                     /locus_tag="OO249_000254"
     CDS             11905..12627
                     /gene="nfsA"
                     /locus_tag="OO249_000254"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5634679.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGPCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            12688..13590
                     /gene="rimK"
                     /locus_tag="OO249_000255"
     CDS             12688..13590
                     /gene="rimK"
                     /locus_tag="OO249_000255"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5634680.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERYATTEYCLKTGG"
     gene            13678..14154
                     /locus_tag="OO249_000256"
     CDS             13678..14154
                     /locus_tag="OO249_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5634681.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            14505..15617
                     /gene="potF"
                     /locus_tag="OO249_000257"
     CDS             14505..15617
                     /gene="potF"
                     /locus_tag="OO249_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5634682.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHNPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            15712..16845
                     /gene="potG"
                     /locus_tag="OO249_000258"
     CDS             15712..16845
                     /gene="potG"
                     /locus_tag="OO249_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5634683.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            16855..17808
                     /gene="potH"
                     /locus_tag="OO249_000259"
     CDS             16855..17808
                     /gene="potH"
                     /locus_tag="OO249_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5634684.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVAAAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            17805..18650
                     /gene="potI"
                     /locus_tag="OO249_000260"
     CDS             17805..18650
                     /gene="potI"
                     /locus_tag="OO249_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5634685.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            18710..19198
                     /locus_tag="OO249_000261"
     CDS             18710..19198
                     /locus_tag="OO249_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5634686.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            19239..20396
                     /gene="rlmC"
                     /locus_tag="OO249_000262"
     CDS             19239..20396
                     /gene="rlmC"
                     /locus_tag="OO249_000262"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5634687.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQSEAAGAASDC"
     gene            complement(20540..21271)
                     /gene="artJ"
                     /locus_tag="OO249_000263"
     CDS             complement(20540..21271)
                     /gene="artJ"
                     /locus_tag="OO249_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5634688.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(21562..22230)
                     /gene="artM"
                     /locus_tag="OO249_000264"
     CDS             complement(21562..22230)
                     /gene="artM"
                     /locus_tag="OO249_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5634689.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(22230..22946)
                     /gene="artQ"
                     /locus_tag="OO249_000265"
     CDS             complement(22230..22946)
                     /gene="artQ"
                     /locus_tag="OO249_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5634690.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(22953..23684)
                     /gene="artJ"
                     /locus_tag="OO249_000266"
     CDS             complement(22953..23684)
                     /gene="artJ"
                     /locus_tag="OO249_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5634691.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYNSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(23702..24430)
                     /gene="artP"
                     /locus_tag="OO249_000267"
     CDS             complement(23702..24430)
                     /gene="artP"
                     /locus_tag="OO249_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5634692.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(24648..25163)
                     /locus_tag="OO249_000268"
     CDS             complement(24648..25163)
                     /locus_tag="OO249_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5634693.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            25289..25612
                     /locus_tag="OO249_000269"
     CDS             25289..25612
                     /locus_tag="OO249_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5634694.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25609..26439
                     /gene="amiD"
                     /locus_tag="OO249_000270"
     CDS             25609..26439
                     /gene="amiD"
                     /locus_tag="OO249_000270"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5634695.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26436..27446)
                     /locus_tag="OO249_000271"
     CDS             complement(26436..27446)
                     /locus_tag="OO249_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5634696.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     FRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGKL
                     PR"
     gene            complement(27545..28975)
                     /locus_tag="OO249_000272"
     CDS             complement(27545..28975)
                     /locus_tag="OO249_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5634697.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28986..29987)
                     /gene="ltaE"
                     /locus_tag="OO249_000273"
     CDS             complement(28986..29987)
                     /gene="ltaE"
                     /locus_tag="OO249_000273"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5634698.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30024..31742)
                     /gene="poxB"
                     /locus_tag="OO249_000274"
     CDS             complement(30024..31742)
                     /gene="poxB"
                     /locus_tag="OO249_000274"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5634699.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31875..32843)
                     /gene="hcr"
                     /locus_tag="OO249_000275"
     CDS             complement(31875..32843)
                     /gene="hcr"
                     /locus_tag="OO249_000275"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5634700.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVHTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAKAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32855..34507)
                     /gene="hcp"
                     /locus_tag="OO249_000276"
                     /gene_synonym="priS"
     CDS             complement(32855..34507)
                     /gene="hcp"
                     /locus_tag="OO249_000276"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5634701.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34651..35550)
                     /gene="lysO"
                     /locus_tag="OO249_000277"
     CDS             complement(34651..35550)
                     /gene="lysO"
                     /locus_tag="OO249_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5634702.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGSLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36044..36739)
                     /gene="aqpZ"
                     /locus_tag="OO249_000278"
     CDS             complement(36044..36739)
                     /gene="aqpZ"
                     /locus_tag="OO249_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5634703.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37165..38823
                     /locus_tag="OO249_000279"
     CDS             37165..38823
                     /locus_tag="OO249_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5634704.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38820..39776)
                     /locus_tag="OO249_000280"
     CDS             complement(38820..39776)
                     /locus_tag="OO249_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HCR5634705.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLKLTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39927..41042
                     /gene="macA"
                     /locus_tag="OO249_000281"
     CDS             39927..41042
                     /gene="macA"
                     /locus_tag="OO249_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5634706.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAEIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41039..42985
                     /gene="macB"
                     /locus_tag="OO249_000282"
     CDS             41039..42985
                     /gene="macB"
                     /locus_tag="OO249_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5634707.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHMVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALVRE"
     gene            complement(43058..43282)
                     /gene="cspD"
                     /locus_tag="OO249_000283"
     CDS             complement(43058..43282)
                     /gene="cspD"
                     /locus_tag="OO249_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5634708.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43605..43925
                     /gene="clpS"
                     /locus_tag="OO249_000284"
     CDS             43605..43925
                     /gene="clpS"
                     /locus_tag="OO249_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5634709.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            43956..46232
                     /gene="clpA"
                     /locus_tag="OO249_000285"
     CDS             43956..46232
                     /gene="clpA"
                     /locus_tag="OO249_000285"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5634710.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46576..46663)
                     /locus_tag="OO249_000286"
     tRNA            complement(46576..46663)
                     /locus_tag="OO249_000286"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46627..46629),aa:Ser,seq:gga)
     gene            complement(46917..47135)
                     /gene="infA"
                     /locus_tag="OO249_000287"
     CDS             complement(46917..47135)
                     /gene="infA"
                     /locus_tag="OO249_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5634711.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47420..48124)
                     /gene="aat"
                     /locus_tag="OO249_000288"
     CDS             complement(47420..48124)
                     /gene="aat"
                     /locus_tag="OO249_000288"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5634712.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48166..49887)
                     /gene="cydC"
                     /locus_tag="OO249_000289"
     CDS             complement(48166..49887)
                     /gene="cydC"
                     /locus_tag="OO249_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5634713.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(49888..51654)
                     /gene="cydD"
                     /locus_tag="OO249_000290"
     CDS             complement(49888..51654)
                     /gene="cydD"
                     /locus_tag="OO249_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5634714.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQCGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51777..52742)
                     /gene="trxB"
                     /locus_tag="OO249_000291"
     CDS             complement(51777..52742)
                     /gene="trxB"
                     /locus_tag="OO249_000291"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5634715.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53287..53781
                     /gene="lrp"
                     /locus_tag="OO249_000292"
     CDS             53287..53781
                     /gene="lrp"
                     /locus_tag="OO249_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5634716.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            53916..57944
                     /gene="ftsK"
                     /locus_tag="OO249_000293"
     CDS             53916..57944
                     /gene="ftsK"
                     /locus_tag="OO249_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5634717.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSAAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQQVAPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            58099..58710
                     /gene="lolA"
                     /locus_tag="OO249_000294"
     CDS             58099..58710
                     /gene="lolA"
                     /locus_tag="OO249_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5634718.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58721..60064
                     /gene="rarA"
                     /locus_tag="OO249_000295"
                     /gene_synonym="mgsA"
     CDS             58721..60064
                     /gene="rarA"
                     /locus_tag="OO249_000295"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5634719.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSSKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60155..61447
                     /gene="serS"
                     /locus_tag="OO249_000296"
     CDS             60155..61447
                     /gene="serS"
                     /locus_tag="OO249_000296"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5634720.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61686..64130
                     /gene="dmsA"
                     /locus_tag="OO249_000297"
                     /pseudo
     CDS             61686..64130
                     /gene="dmsA"
                     /locus_tag="OO249_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64141..>64376
                     /locus_tag="OO249_000298"
     CDS             64141..>64376
                     /locus_tag="OO249_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5634721.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKV"
BASE COUNT        15833 a        16156 c        17334 g        15053 t
ORIGIN      
        1 acaaattata ttgcgccagt tcctcacctt tcagacgacc aatagcggct ttaccgggcc
       61 attgtttact gttcatgtat tcgttggcct gattaagcaa taattgcctt tctgattctg
      121 cctgttgaat aagttcttca tatgatggtg gaggtattag tgcccatgtg ggtaatccat
      181 tttttcctgc aacacgaatt ttgtcatctg ggggcgtatt gattgcaaat tcattataaa
      241 catcatcact gacagccaga gcatcatctg gccatgaatt tgcattaatg taatcatcct
      301 taagcgcagg attcacaaaa atgtttaaag atggactata aaacatatta aacccctata
      361 gcgatataac gacctaatac agcatttgca gcagtcgcta tgcttgaaaa accgcggaac
      421 tgatttgatg taattgcaga agccgacagg attcctgcgc ctgaaggtgt atgccccaca
      481 tgactaacta tcatcccata acatgctgac ggaaaggcaa aaggaaaatc attaagatat
      541 cctgcatctt caccaccaga cccaccaaat ctcgcctgcc cccactgaat aatcagtgtt
      601 ctccgggaac ctgaaataat taacggaatc gttacatacc cattcaggcc aatgacaccc
      661 gatgcagtgc cagccagaga taattctccc aaaccaacgt ttaagaaaat gcagagataa
      721 tggctaactg gcatcatccc cggtttttat tcaggggatt gatcatgctt attggctatg
      781 tacgcgtgtc aacaaatgac cagaacaccg atttgcaacg taatgcactg aactgcgcgg
      841 gatgtgagcg gatttttgag gataaaatca gtggcactaa gcccgacaga ccggggctta
      901 aaaaactgct caggacacta tcggcaggag acacgctggt tgtctggaag ctggacagac
      961 tgggacgcag tatgcggcat cttgttacgc tgatagaaga gttgcgccag cgtggtgtga
     1021 atttccgaag cctgactgac agtattgata ccagtacccc aatgggccgt ttcttttttc
     1081 acgtcatggg ggctctggct gaaatggaac gtgaactgat agttgaacgt accagagcag
     1141 ggctggctgc agctcgtgcc aaaggcagag taggtggacg ccgtcctaag ttgaccaccg
     1201 aacagtgggc acagattgga cgtttactcg aggccggaga atcaagacag cgtattgcac
     1261 tgatttttga tgtgggcgtt tctaccattt atagaaaatt tccggcgaat gagagcaatg
     1321 aatcgccctg agtcagtatt attttgattt ttcctgtaag cggacaaata ccgtcatttt
     1381 gtgtgaataa cgacacaact gcgcttagct gtttgtcagg cacaatcact tcaacatagg
     1441 gcgaagccta atccaatcag gaggttcgcc actatggctc aggattacca ccacggggtg
     1501 cgcgttgttg aagtcaacga aggcacccga tccattacca cggtgagcac cgccatcgtg
     1561 ggtatggtct gcacgggcga tgatgccgat gcaaaaatgt ttcctcttaa taaacctgtg
     1621 ctgatcactg atgtgctgac cgccagcggt aaagcgggtg agtctggcac gctggcccgt
     1681 tcgctggatg ccatcgctga ccaggcaaaa cccgtgaccg ttgttgtgcg tgtgccgcag
     1741 ggtgaaacgg aagaagaaac cacaaccaat atcattggcg cagtgaccgc cgagggtaaa
     1801 aaaacaggta tgaaagccct gttatctgcc cagtcacagc tcggcgttaa accgcgcatt
     1861 ctcggcgtgc caggtcacga cacgaaggcg gtagctactg agttgctgag cgtggcgcaa
     1921 agcctgcgtg gatttgctta cctgtcagcg tatggttgca agacggtgca ggaagcaatc
     1981 acttaccgtg aaaacttcag tcagcgtgaa ggaatgctga tctggcccga ctttactggc
     2041 tgggacacgg tgctgaatgc cgaagcaacg gcttatgcca ccgcccgtgc gctcggtctg
     2101 cgcgctaaaa ttgatgagca gaccggatgg cacaaaagcc tgtccaacgt gggcgtgaac
     2161 ggtgtcaccg gaatttctgc tgatgtgttc tgggatctgc aggacccggc aaccgatgca
     2221 ggtctgctta accagaacga tatcaccacg cttatccgca aagacggttt ccgcttctgg
     2281 ggttcccgct gtctgagcga tgatccgctc tttgccttcg aaaactacac ccgcacggcg
     2341 caggtgctga tggacacgat ggcagaagcg cacatgtggg cggtggacaa accgctgaac
     2401 ccgtcgctgg cccgcgacat tatcgaaggt atccgcgcca aaatgcgtag cctggtcagt
     2461 cagggctatc tcattggtgg tgattgctgg ctggacgagt cggtgaacga taaagacacg
     2521 ctgaaagccg ggaaactcac catcgattac gactacacgc cagtgccgcc acttgaaaac
     2581 ctgatgctgc gtcagcgcat caccgatcag tacctggtga atttctccag ccaggtcagc
     2641 gcgtaagggg acaacatggc tttaccacgc aaattaaaac acctgaacct gtttaacgac
     2701 gggaacaact ggcaggggat cgttgagtcg ctgacgctgc cgaaattcac ccgcaaatat
     2761 gagaagtatc gcggcggcgg tatgcccgga gcggtggatg tggatctggg actggatgac
     2821 agtgcactgg atacagaatt ttccattggt ggtactgaac tgctgctgtt taagcagatg
     2881 ggcaaagcca cggtggatgg catccagctg cgctttaccg gctctattca gcgtgatgat
     2941 accggggaag tgcaggccgt ggagcttgtg gtgcgtggac gtcacaaaga agtagattcc
     3001 ggcgagtgga agacgggcga aagcaacact accaaagtga ccagtaccaa cagctacgcg
     3061 aagctgacca tcaatggtga ggtgctctat gaagtggacc tgatcaacat ggtggaaatt
     3121 gtggacggtg tggacctgat ggaagcgcac cgcaacgccc tcggcctctg atgtatctga
     3181 acggcgcgga atgccgcgcc agaacctaat ttacaggaca acaaaatgag cgataagcag
     3241 actgaaaaga ccattcaact ggatactccc atcatgcgcg gtaaaacaga aattaccgaa
     3301 attgtgttgc gtaaaccgca gtccggtgcg ctgcgcggca cacgcctgca ggccattatg
     3361 gatatggatg tgaacgcgat gatgaccgtg atcccccgca tctccagtcc ggcactgact
     3421 gcacaggaaa ttgcagagat ggacccggca gatctcactg ccatgtcggt tgaggttgtc
     3481 acttttttgt tgaagaagtc ggtgcttgcc ggtttaccga cagcctgacg gttgacgatc
     3541 tggtggctga tatcgccacc atctttcact ggccgccatc cgttactgac gttatgccgc
     3601 tgaccgaagt gctggaatgg cgggataaag cgattcagag aagcggggcc agccatgagt
     3661 gacaataacc tgcgtctgca ggtgattctt aatgcggttg acaaactcac ccgcccattt
     3721 cgatccgcgc aggccagttc aagagaactg gctgctgctg tcaaaaaatc ccgcgatgca
     3781 ataaagcagc ttgatcaggc cgggagcagt ctggacagct tccgaaagct gcaggcagaa
     3841 aatcagaaat taggcgacag gctgaactat gcccgccagc gtgcaaattt gctcagtcag
     3901 gaactgggag cgatggggcc gccttcgcaa cgtcaggttg ttgctctggg ccgtcaacgg
     3961 ctggctgttc agcgcctaga agaacgccag aaaaagctgc agcagcagac ggcgcttgtg
     4021 cgtgcagaac tttatcgtgc tggtatttca gctaatgatg gcgccagtgc gacggcccgc
     4081 attacccgtg aaacgatgcg ttataacagg cagctttctg agcaggaagc gaggttacga
     4141 cgtgtcgggg agcaacagcg aaaaatgcac gccgcccggg gggcttacgc caggcgtctt
     4201 gaggtaaggg atcgtattgc aggtgccgga gccaccacca cggctgcagg gctggcaatg
     4261 ggcgcaccag tgatggcggc agtaaaaagc tataccagca tggaagatgc catgaaaggt
     4321 gtggcaaagc aggtcaatgg tctgcgtgac gataatggca accgcactgc gcgtttttac
     4381 gaaatgcagg gtgccatcaa ggctgccagc gaacagttgc cgatggaaaa cggtgctgtg
     4441 gacttcgccg cactggttga aggtggtgcg cgcatgaacg tcgcaaaccc tgacgacagc
     4501 tgggaagacc agaaacgtga cctgctggcc ttcgccagca cggcggcaaa ggcggcaaca
     4561 gcctttgaac tgccagcgga tgaactgtca gaaagtctgg ggaaaatcgc ccagctctac
     4621 aaaatcccta cccgcaatat tgagcagctc ggtgatgcgc tgaactatct ggatgataac
     4681 gccatgtcga aaggggcgga catcattgat gtcatgcaac gcctgggcgg tgtggctgac
     4741 cgtctggatt atcgtaaagc ggcggcgttg ggttccacct tcctgacact cggcgctgcg
     4801 cctgaggttg cagccagtgc agcaaacgcg atggtgcgtg aattgtccat tgccaccatg
     4861 caaagcaaga gtttctttga agggatgaat ctgctgaaac tcaatccaga agtgattgaa
     4921 aagcagatga cgaaggatgc gatgggaact atccagcggg tgctggagaa ggtgaatgcg
     4981 ctgccacagg ataagcgcct gtctgccatg accatgttgt ttggtaaaga gtttggcgat
     5041 gacgcagcga aactggcaaa caacctgccg gaacttcagc gccagttaaa actgactgcg
     5101 ggcaatgatg cgctcggttc catgcagaaa gaatccgaca tcaacaagga ttcactttct
     5161 gctcagtggt tgctggtcaa aaccggagca cagaacacct tcagcagcct gggggaaacg
     5221 ctgcgccagc cgctgatgga tattctgtac acggtgaaaa gcgtcacggg ggcgttgcgt
     5281 cgctgggtgg aagctaaccc ggaactgacg ggcactctga tgaaagtagc cgctgtggta
     5341 gctgccgtta ccgtgggcct cggcacctta gcggtggcgc tggctgcagt gctggggccg
     5401 ctggcagtca tccgtctggg attctctgtg ctgggtatca aaacgttacc ttccgttacg
     5461 gcaacagtaa cccgaaccag caacgcgttg tcctggctgg ctggcgcacc actggcactg
     5521 ctgcgacgcg ggcttgcttc atcgggcaac gctgcaggtt tacttactgc gccgttgtcg
     5581 tctttgcgcc gcacggcatc actgacggga aatgtcctga aaactgtagc aggtgcgccg
     5641 gttgcacttt tgcggtctgg attatccggt ttacgtgcgg ttgctgtgat gtttatgaat
     5701 ccactggcgg tactacgcgg cggactggct gccacaggca cggtgctgcg tatgctcgca
     5761 tccggtccac tggcgatgct gcgcgttgcc ctgtatgccg tctctggtct gttaggtgct
     5821 ctgctcagtc tgataggtct tgtggttact gcactggcgg gtgtggcgct ggttgtctgg
     5881 aaatactggc agcccatcac cgcatttctc ggtggcgtgg tggaaggatt caaagcggcg
     5941 gcaggtcctg tcagtgccgc gttcgaaccg cttaagcctg tgttccagtg gattggcgac
     6001 aaagtacagg cgctgtgggg ctggtttact gatctgctga cgcccgttaa gtcgacctct
     6061 gccgaactgc agagtgcagc ggcaatgggg cgacgattcg gggaggctct ggcggaaggg
     6121 ctgaatatgg tcatgcatcc gctggactcc ctgaaatccg gcgtttcctg gttgctggag
     6181 aaacttggca ttgtcagtaa agaggccgca aaggcgaaac tgccggaaag cgtgacgcgt
     6241 cagcaacctg cgacggtgaa tgcagacggt aaagtgatga tgccatcggg tggttttccg
     6301 tcatggggat atggctttgc ggggatgtat gacagcggcg ggtatatccc gcgcgggcag
     6361 tttggcatcg tcggtgaaaa cgggccggaa attgttaacg gcccggcaaa tgtgaccagc
     6421 cggagaaata cagctgcact ggctgccgtt gttgccggaa tgatgggtgt tgctgctacg
     6481 ccagcagagc ttccaccgtt gcaccctttg gcacttcccg cgaaaggcgg tgaagcgatg
     6541 gtgagtcgtg cagccactgt gccgcccgtt caacggattg aggcaccgat gcagatcatc
     6601 attcaaacgc agccaggaca aagtgcgcag gatattgcgc gggaggtggc ccgccagctt
     6661 gatgaacgtg aacgcaggct gaaggcaaaa gccaggagta actacagcga tcagggggga
     6721 tacgacgcat gatgatggtg ctgggattgt acgtgtttat gctgcgcacc gtgccgtatc
     6781 aggagctgca gtatcaacgc agctggcgac atgcggcaaa cagccgggta aaccgacgcc
     6841 cgtcaacgca gtttctggga ccggacaacg acatgctgac gctttctggt gttcttatgc
     6901 cggaggtaac gggcggcagg ctgtcgttgc tggctctgga gcagatggca gaacagggaa
     6961 aagcatggcc cctgattgaa ggcagcggca cgatttacgg tatgtatgtg attgagggac
     7021 tgaatcagac taaaacggag tttttccgcg acggtatgcc gcgccggatt gagttcaccc
     7081 tgtcgctaaa acgcgtggat gaatccctgt ccgatatgtt cggtgatctc agtgcgcaac
     7141 tgaataatct gcagggaacg gcaacatctg ccttaagcga tatcagtaaa acggtgggag
     7201 ggctgctgtc gtgaatttca gctctgaact gcttaacaaa ggcaacaaaa ctccggcatt
     7261 cagcatcagt attgaaggca gggatatcac cactgtgctg gacaaccgcc tgatggggct
     7321 gacgctgacg gataaccggg gctttgaagc ggaccagctt gatctggagc tggacgacgc
     7381 cgatggaaaa atcgtgctgc cgcgccgtgg ggctgtcatt acgctggcgc tgggctggaa
     7441 gggacagccg cttttcccga aaggggcatt cacggtggac gagattgaac acactggcgc
     7501 accggatcgc ctgactatcc gggcgcgaag tgctgatttt cgtgaaacgc tgaatacccg
     7561 ccgtgaaaag tcgtggcaca agaccaccgt tggggaagtg gtgaaggaaa tagctgcgcg
     7621 gcacaaactg aagatggcat tgggtgaaga cttgtcggat aaactcgtgg agcatataga
     7681 ccagactaat gagagtgacg gcagtttttt gatgcggctg gcgcgccagt acggtgctat
     7741 tgcgtcggtg aaaaatggca atctgttatt catccggcag gggcagggta aaagcgccag
     7801 cggtaaacca ctaccggtga tcactatcac acgcaaggac ggcgacagtc accgctttac
     7861 cctggcagat cgcggagctt acacgggcgt cattgccagc tggttgcata cccgcgaacc
     7921 cgcgaagaaa gaaagcacca cggtgaagcg taagcgcagg actaagaagc agaagaaaga
     7981 gccggaagcg aagcagggcg attacctggt gggtacggat gaaaacgtgc tggtacttaa
     8041 tcgcacttat gccaaccgga gcaacgccga acgggcagcg aaaatgcagt gggaacgcct
     8101 gcaacgcggc gttgcatcat tctcgctaca actggcggaa gggcgggcag atctctacac
     8161 agaaatgcct gtgaaggtca gtggctttaa acagccgcta gatgatgcgg aatggaccat
     8221 tacgactctg acgcatactg tcagcccgga taacggtttt acgaccagtc tggagcttga
     8281 agtgaggatt gatgatttcg aaatggaatg attcttcgca atggagaact tttaagtttg
     8341 caaaatggaa taatgcggta tcattattgt gaatttagca aaaatgggga gaactcgaaa
     8401 aatgatgatt tgcccactgt gtggaagtgc cgcccatact cgcagcagtt ttcaggtatc
     8461 ttcattgacc aaagagcgtt acaaccagtg ccagaacatt aactgcagcc atacttttgt
     8521 tacccatgaa acttttgttc gttcgattgc aacgccaaaa gagtcaaatc cggttcagcc
     8581 gcatccaatg aaatcaggac aggtggcgct ctctctttga cgctgccgcc attttgtcgc
     8641 catcgttaaa aaacagtgct tctaacatca tgattttaaa aggcataaat ttcagacaac
     8701 aaaaacccat caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca
     8761 tcaaagaaaa gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt
     8821 gcaaaaaaat ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga
     8881 cgatgattgt ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc
     8941 ccagcgcagg gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg
     9001 tgcgtgcgcc catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac
     9061 cgaacaagaa acagataacc acgggcacaa gactgacaat taatccggca atcaacatct
     9121 gaccgccaat cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg
     9181 ccataaacac catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac
     9241 cgaaggtcgg gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg
     9301 cagcgttccc catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca
     9361 gcccaataac aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga
     9421 taaagccgat gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa
     9481 cgtcaccttt gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga
     9541 cgcggttaag gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag
     9601 cgttatggtt tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat
     9661 cgaaaacttc tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc
     9721 ctaccaacgc tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa
     9781 tcccgttacg tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac
     9841 gcagattttt gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca
     9901 ccggtaaata aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct
     9961 gggcgctggt ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca
    10021 aactcaccag accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca
    10081 gtgagagctg cctgctttcc atgccggaat gacgcagtgt atcgccagca ccgaccagaa
    10141 ccggtgtcga cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa
    10201 acagcttacc taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca
    10261 ttaggtaatt tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc
    10321 cgacgcagaa aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat
    10381 gttgttggcc taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg
    10441 aaccaagtcg taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa
    10501 tgtaattccc atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta
    10561 ctagtaagct gttgaaagaa atggtaattt acgataatgt tttttaccag aattcagggc
    10621 gcagattcat tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat
    10681 cacattaggt atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag
    10741 cggctttatc ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag
    10801 gaggaacgag tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc
    10861 attgtggtgt gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat
    10921 cctgaaatcg gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag
    10981 cgtagagaag tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc
    11041 attatgcggc tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta
    11101 agcgcggtgt tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt
    11161 aaaccacagc acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag
    11221 gcgtccagat tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga
    11281 tcgacaaaaa tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct
    11341 ttagtgatcc cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg
    11401 ctcaatttct cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa
    11461 ataacggttt gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat
    11521 gtattcgctg aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa
    11581 cacaatacag tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg
    11641 cgatcggtaa attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg
    11701 cggtggcgct gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag
    11761 aagtgcagat tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg
    11821 ttattcttca ggtggcaaaa cgtcttgccc cacagctgat gaaccgtcca ccgcaatatt
    11881 cacgttcaga aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc
    11941 tccattcgcc atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac
    12001 agcgcccgtg cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc
    12061 gacaaagcat tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa
    12121 gcggcggagt tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat
    12181 gctcagctcg gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg
    12241 cagaatgcat taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg
    12301 cgcaataata ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg
    12361 tttgggccgt gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc
    12421 tccattttgg tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat
    12481 gacgagcaac tggcggaata ttacctcacc cgtggcagca ataatcgccg ggatacctgg
    12541 agcgatcata tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg
    12601 cacaaacagg gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt
    12661 tgaccaggtc tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg
    12721 ctctattcgt gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt
    12781 cttgatccgc tttcttgcta catgaacata aatcctgcgg cgtcttctat tcactacaaa
    12841 ggccgcaagt taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt
    12901 tatgggacgg cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg
    12961 gtcgccattg cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc
    13021 atcgacctgc ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac
    13081 atggtcggtg gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc
    13141 gtgctggcgg agacgcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac
    13201 gcgcatattc tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg
    13261 gttgttggcg atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg cgattttcgt
    13321 tccaatttgc atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa
    13381 atcgcgataa aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt
    13441 gctaatcgcg ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa
    13501 aaaaccactg gtatcgacat cgcgggtaaa atgatccgct ggatcgaacg ctacgctacg
    13561 acagaatatt gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc
    13621 ctgcgctttt tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg
    13681 acatcgctgg tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg
    13741 agtttttttg aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac
    13801 ggtgtctttg atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg
    13861 gaagtccgac cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt
    13921 tcgctgaccg tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt
    13981 tgccagtctt tatccgttat gcagggcgta acctacgagc agtttgcatg gttcgtgcgt
    14041 cagagcgaag agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta
    14101 ccgactgacg atgaagggca aaacaacgtt accgaaaact atttcctcca ctgataactc
    14161 ctttcgagca cgcagtcgct ggtgcagtga ctgcgcactg caaaattatc tgctgttttt
    14221 aaccttttct taaagattat ttcacttctc ttgtgccgat ttggctttat cacatagagc
    14281 aaatacgcat aacaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct
    14341 ggctaaagtt attcttgcga tgcttttata tagcgagcag tgctggccgg gagagagtta
    14401 tcttttctta caccgcgccg ataaaaaata tgcacgttta ttgcatatct tttagtatga
    14461 caacttttgt tcgtttgtta acgaactttc agaaggaaag agatatgacc gccttaaata
    14521 aaaaatggct ttcgggtctg gttgcgggtg ctctgatggc cgtctctgtc ggcacgctcg
    14581 cggctgaaca aaaaacactc cacatttata actggtctga ttatatcgcc cctgacacgg
    14641 tggccaattt tgaaaaagaa accggtatta aagtcgtcta cgatgttttc gactctaacg
    14701 aagtactgga aggcaaatta atggcgggta gcaccggctt tgacttggtg gttccatctg
    14761 ccagctttct tgaacgccag ttgactgcgg gagttttcca gccgctggac aaaagcaaat
    14821 tgccggagtg gaagaatctc gacccggagc tgctgaagct ggtcgccaaa cacaatcccg
    14881 acaataaatt tgctatgccc tatatgtggg cgacgaccgg gattggctat aacgttgata
    14941 aagttaaagc tgtgctgggc gaaaacgcgc ccgtcgatag ctgggacttg atcctcaaac
    15001 ctgaaaatct ggaaaaactg aaaagctgcg gtgtctcttt cctggatgcg ccagaagaag
    15061 tttttgctac cgtgttgaat tatctcggca aagatcccaa cagcactaaa gcggatgatt
    15121 acaccggacc ggcaaccgat ctgctgttaa agctgcgccc gaacattcgt tatttccatt
    15181 catctcaata cattaacgac ctggcaaacg gcgatatttg cgtcgctatc ggctgggcag
    15241 gtgatgtctg gcaggcgtca aaccgcgcga aggaagcgaa gaatggagtg aatgtctcgt
    15301 tctcgattcc aaaagaaggg gcgatggcgt tctttgatgt attcgccatg cctgcggatg
    15361 ccaaaaacaa agacgaagcc tatcagttcc tgaattacct gctgcgcccg gatgtagtag
    15421 cgcatatttc cgaccatgtg ttctatgcca acgccaataa agcagccacg ccgctggtga
    15481 gtgcggaagt ccgtgagaac ccgggtattt atcctccggc ggacgttcgt gcgaagctat
    15541 tcactctgaa agtacaggat ccgaaaatcg accgtgtgcg cacccgcgcg tggaccaaag
    15601 tgaagagcgg aaaataatcc gcagccttag atgccgggcg ggcgcaccac acccgccggc
    15661 aattcgcacc atcatggtgc gcttgcacac attcaatgcc ggagagcagc cgtgaatgac
    15721 gctatccctc gcccgcaggc gaaaacccgt aaggcgctga cgccgctatt agaaatccgc
    15781 aacctcacca aatcctacga tggtcagcat gcggtcgatg atgtcagcct gaccatctac
    15841 aaaggtgaaa tcttcgcgct gctgggcgct tccggctgtg gcaagtccac gctgctgcgt
    15901 atgctggcag gttttgaaca accttctgcc gggcagataa tgctcgatgg cgtcgatttg
    15961 tcacaggtgc cgccttacct gcgccccatc aatatgatgt ttcagtctta cgcgctgttt
    16021 ccgcatatga ccgtggaaca gaacatcgct tttggcctga aacaggacaa actaccgaaa
    16081 gcggaaattg ccagtcgggt ccatgagatg ctcggcctgg tgcacatgca ggagttcgcc
    16141 aaacgcaaac cgcatcagct ttccggtggt cagcgacagc gtgtggcgct ggcccgaagc
    16201 cttgccaagc gcccgaaact attactgctc gacgagccga tgggcgcgct ggataaaaag
    16261 ctgcgtgacc ggatgcagct tgaagtggtg gatattctgg agcgtgtcgg cgtgacctgt
    16321 gtgatggtca ctcacgatca ggaagaggcg atgacgatgg cgggacgtat cgccattatg
    16381 aatcgcggga aatttgtcca gattggcgag ccagaagaga tctacgaaca tccaaccacc
    16441 cgctacagtg ctgaatttat cggttcggtt aatgtctttg aaggtgtact caaagagcgt
    16501 catgaggatg ggcttgtgct ttattcgcct gggctggtgc atccactgaa agtcgacgcg
    16561 gatgcctcgg tggtcgataa cgtgccggta catgtggcgc tgcgcccgga aaaaatcatg
    16621 ctttgcgaag agccgcccgc caatggttgt aacttcgcgg tgggggaggt gatacacatt
    16681 gcctatctcg gcgatctttc ggtgtatcac gttcgtctga aaagtgggca gatgattagc
    16741 gcccagctac aaaacgccca tcgtcatcgt aaagggttac cgacctgggg cgacgaagtg
    16801 cgtttgtgct gggaagtgga cagctgtgtg gtgctgacgg tttaaggagc aaagatgaat
    16861 acacttgaac ctgctgccca gtcgaaaccg ccgggcggat ttaagctgtg gttgtcgcag
    16921 ctgcaaatga agcatgggcg caaactggtc attgcgttgc catatatctg gttgatcttg
    16981 ctgtttctgc tgccatttct gattgtcttt aaaataagcc tggcggagat ggcgcgcgct
    17041 attccacctt ataccgaact gatggagtgg gctgacgggc aactgtccat cactcttaat
    17101 ctcggtaatt ttctgcaact gaccgacgat ccgctctatt tcgatgctta tctccagtcg
    17161 ctacaggtgg ctgcgatttc gacattttgc tgtttactga tcggctatcc gctggcgtgg
    17221 gcggtggcgc acagtaagcc ttcgacccgt aatattttat tactactggt gatcctgccg
    17281 tcgtggacct cgtttctgat ccgcgtttat gcctggatgg gaatattaaa aaacaacggt
    17341 gtgctgaata attttctgct gtggctgggg gttatcgatc aaccgctgac cattcttcat
    17401 accaatctgg cagtttatat tggcattgtt tacgcctacg tgccgtttat ggtgctgccg
    17461 atttataccg cgttgattcg tattgattat tcgctagtgg aagcagcgct ggatctcggt
    17521 gcacgaccgc tgaaaacgtt ctttactgtg atcgtgccgc tgactaaagg tgggattatt
    17581 gccggatcga tgctggtgtt tatcccggct gtgggcgagt ttgtgatccc ggaactgctc
    17641 ggtggcccgg acagcatcat gatcgggcgc gtgctatggc aagagttttt caataaccgc
    17701 gactggccgg tggccgcggc ggtagcgatc atcatgttgc tgctgctaat tgtgccgata
    17761 atgtggttcc acaaacacca gcaaaaaagc gtgggagaac acggatgaat aatttaccgg
    17821 tagttcgttc gccctggcgg attgtgattt tgctgctggg ctttaccttt ctctacgcgc
    17881 caatgctgat gctggttatc tattcgttta acagctcgaa gctggtgacg gtgtgggccg
    17941 gctggtcaac gcgctggtat ggtgagttat tgcgcgatga cgcgatgatg agtgcggttg
    18001 gtttaagcct gacaattgct gcctgtgcgg caacggcggc ggcgatcctc gggactattg
    18061 cggcggtggt gctggtgcgc tttggcaggt ttcgcggatc aaatggcttt gcctttatga
    18121 tcacagcgcc gctggtaatg ccagatgtca tcacgggctt gtcgctgttg ttattattcg
    18181 tcgcgcttgc tcatgccatt ggctggcctg cggaccgcgg tatgcttacc atctggctgg
    18241 cgcatgtcac tttctgtacg gcttatgtgg cggtcgttat ttcgtcgcgt ctgcgggaac
    18301 tggatcgctc gatagaagaa gcagcgatgg atctcggtgc gacgccgctg aaagtgtttt
    18361 tcgtcattac gctaccgatg atcatgcctg cgatcatttc tggctggtta ctggctttta
    18421 ctttgtcgct tgatgatctg gtgatcgcca gctttgtttc cgggccggga gccaccacgt
    18481 taccgatgct ggtcttttcc agcgtgcgga tgggggtgaa tccggaaatc aacgcccttg
    18541 caacgttaat tctcggtgcg gtcggaattg tcggatttat cgcctggtat ctgatggctc
    18601 gcgcagaaaa acagcggata cgcgatatcc agcgtgcaag acatggctga agacactaaa
    18661 atttgccaac ctggctacat aatgccgcgc atgccgcggc attgttttca tggaagacga
    18721 aacgttggga ttttttaaga aaacatcttc atctcatgct cgcctgaatg tgcctgcgct
    18781 ggtgcaggta gcggcgctcg ccattattat gatccgtggc ctcgacgtgc tgatgatttt
    18841 caatacgctg ggcgtgcgcg gtattggcga gttcattcat cgcagcgtac aaacctggag
    18901 cttaacgctg gtatttttaa gcagtctggt gctggttttt atcgagatct ggtgtgcgtt
    18961 ttcactggtg aaagggcgtc gctgggcgcg ctggctatat ctgctgacac aaatcaccgc
    19021 cgcaagttac ttgtgggcgg cttcgctggg gtatggttat ccggagctgt tcagcattcc
    19081 cggtgaatca aaacgtgaaa tcttccatag cctgatgctg cagaagctgc cggatatgct
    19141 catcctgatg ctgctgttcg ttccctcgac cagtcggcgg ttcttccagt tgcaataatg
    19201 tgtataatcg tcgcccctga tgatgtgaag gtcaatgtat gcagtgcgca ctttacgacg
    19261 cgggtcgctg tcgttcctgt cagtggataa cgcagccgat tccagagcaa ctctccgcta
    19321 aaaccgccga tcttaaaaat ctgctcgccg attttccggt tgaggaatgg tgcgcgccgg
    19381 tgtcaggccc ggaacaaggg tttcgtaata aagcaaaaat ggtggtgagt ggtagcgttg
    19441 aaaaaccact gctcggtatg ctgcatcgcg atggcacgcc ggaagacctt tgtgactgcc
    19501 cgctttatcc tgcctctttt gcgcccgttt ttgcggcgct aaaaccgttt atcgcccgtg
    19561 cggggttaac gccctacaac gtggcgcgta agcgtggcga actgaaatac attctgctga
    19621 ctgaaagcca gagcgatggc ggcatgatgc tgcgctttgt actgcgttct gaaaccaaac
    19681 tggcacaact gcgtaaggcg ctgccgtggt tacaggaaca actaccgcaa ctgaaagtta
    19741 ttaccgtcaa tattcagccg gtacatatgg cgattatgga aggggagacg gagatctacc
    19801 tgactgaaca acaggcgctg gcagagcgtt ttaatgacgt accgctgtgg atccgtccgc
    19861 aaagtttctt ccagactaat ccggcggtcg ccagccagct gtacgctacc gcgcgcgact
    19921 gggtacggca gctgccagtt aaacatatgt gggatctgtt ctgcggtgtg gggggctttg
    19981 gtttacactg cgcgacgcct gacatgcagt taaccgggat cgaaattgcg ccagaggcca
    20041 ttgcctgtgc aaagcagtca gccgctgaac tgggcttaac tcgtttacaa tttcaggcgc
    20101 tggactccac tcagtttgcc actgctcagg gggaggtgcc ggagctggtg ctggttaacc
    20161 cgccgcgccg cggcattggt aaaccgctgt gtgattatct ctcaacgatg gcaccgcgtt
    20221 ttatcatcta ctccagctgt aacgcccaaa ctatggcgaa agatatccgc gaactgcctg
    20281 ggtttcgcat cgaacgggta cagctttttg atatgttccc gcacaccgcg cactatgaag
    20341 tgctgacgct gctggtgaag caatctgaag ccgcaggtgc ggcttcagat tgctgatcag
    20401 gtgcgctttg ttcatgccgg atgcggcgtg tacgccttat ccggcctaca aaatcatgct
    20461 aaatcaatac attgcagagt tgatgtgggc cagataagcg cagcgcatca ggcaatctta
    20521 cttttgtcat cagtctcaat tactgcggga accactggtc attgattttc tgatatgtac
    20581 cgtcagcttt aattgctgcc agcgcgttat tcagtttttc cagcagagct ttgttatccg
    20641 gacgtacagc gatgcccaga ccggtgccga agtattgcgg atcggtcact ttttcagtag
    20701 caacgcccag ttgcggattg gttttcagcc attcgtttac caccgctgtg tcaccaaata
    20761 ccccatcaat acgaccattt ttcagatcga taaaggcatt ctgataactg tcataggaga
    20821 cagttttcac ttcggggtgc tgatcctgaa tatatttctg gtgcgtggtg ccgttttcca
    20881 tcccaatacg tttgcctttc agatcggcaa acgttttgta ggtatctttt ttggcaatca
    20941 cgacggctga gttttcatag tagggcgtgg taaacgatac ctgcttgcta cgctcgggcg
    21001 tgatatccat accggagatt acggcgtcat attttctgaa tttcagtgac gggatcaggc
    21061 tgtcgaacgc gtgattagta aaagtacatt ctgcctgcat ttgtttgcac aaggcttttg
    21121 ccagatcgat atcaaagccg acaatctcat tattagcacc tatagattca aagggtggat
    21181 aggtggctga aacgccaaaa ttgattttct ctgcggcaga agcaccgaaa gtaaaggaag
    21241 caagtaaagc ggcaagaact aactttttca tgatggaact cccgtctgtc aatcttatga
    21301 tttttggccg tgtctgcggc atgggataac aatgccatca agtgaattta tatgcaataa
    21361 atatgattaa ataatttaaa tgaaataaaa aacgacggat aacttagtga gttgtccgtc
    21421 ttcattataa gaatttatgc actatgtagg ccggataagg cgtccacgcc gcatccggca
    21481 caggcaccgt gctgatgtct gatgcgacgc tggcgcgtct tatcagacct acaaaccccc
    21541 cggcgaatgt acgcagctac attaattccg ccgttcgaat gccagtgctt tgcgctcgat
    21601 cagacgcatc atcagcgtca gcaggccgtt gacgaccagg taaataatcc ctgccgcacc
    21661 gaacaccatt acatcgtagg tgcgtccgta caacaactgg ctgtatccca tcacttccat
    21721 cagcgtaatg gtgtaagcca gagaggtact tttgaacacc agcaccactt cgttggaata
    21781 agaagagagc gagcgtttaa aggcatacgg cagcaggatc gccagcgtat cttttttgct
    21841 cattcccagg gcgctacagg actgccactg accttctggg atcgcacgaa ttgcaccgta
    21901 aaacagctgc gtggtatacg ccgcactgtt cagcgataac gcaatcagcg cacataacca
    21961 cggttctgac aacaaatgcc acagtgccgg atactcctgc aaagtcggaa actggcccgg
    22021 cccgtaataa atcaggaaga tctgcaccag cagcggcgta ccggtaaaca gcgtgatata
    22081 accccgcacc agccacacca gcaccggcgt tttcagcgtc aggatgatgg taaaaatcaa
    22141 tgccagaatc agtgccacaa tcagcgaggc aacggttagc gtcaggctgg tgtgtagccc
    22201 tttcatcagt tcgggtaaat actcaaacat tagctgggcc tccgctcaaa acgtgtcgcg
    22261 cgcaggtcaa tgcgtttgag aatgtactga ctgagcaggg tgatcaccag gtaaatcgcc
    22321 gccgccacaa tataccaggt aaatggttcc tgggtacgag tagcgatgct ttttgtttgc
    22381 agcattaaat cattcacact aatcaaactg accagcgcgg tatctttcag cagcaccagc
    22441 cactggttac cgaggccagg cagcgcatga cgccacatct gcggcatcac cagacggaaa
    22501 aagatagccg atttcgacag ccccagcgcc tgaccagact cccactgacc caccggcacc
    22561 gctttcaacg cgccccgaag cgtttgcgag gcataggcgg catacagcag tgacagagcg
    22621 atgacaccac aaaggaacgg gctaacgtcg aagttctcaa tgtccatctg cactgggatc
    22681 tgcacgaacc caagattgat agtgaagcca tccgaaagcg tcagcagcag ctgcgaggag
    22741 ccaaaataga taaacagcac caccagaatt tctggcaggc cacgcagaat ggttaccagc
    22801 gctgaacctg cccacgcgac aggacgccat ttttccgact cccataccgc aaagaacatc
    22861 gccagcgcca gcccgacaat caatgcacaa acggcaaggc cgacggtcat cccggcggcg
    22921 cttgctaaag gaaaaaattc attcatcagg aattacttct ggaaccattt gttgtagatg
    22981 gtttcgtaag tgccatcttt cttcactttt tccagcgcag tgttgagttt ctgctgcagc
    23041 tcagtgttgc cctgacgtac cgcgatgccg aggccagtgc cgaagtaatc tttatcggtc
    23101 actttgtcgc ccaccgccgc cagtttcggg ttatctttca gccactcagt gaccactgcg
    23161 gtgtcaccga agacgccgtc gatacgcccg ttttgcagat ccagttttgc gttctggtag
    23221 ctgttatacg gaacggtagt gatttccggg tgcttatcca taatgaattt ctggtgtgtc
    23281 gtcccgttct gtacgccgac ttttttgcct ttcagctgat caacactggt gtatttgcct
    23341 tgctgaccca caaacagggc agagttgtca tagtacgggg tggtaaacag cacctgcttt
    23401 tcacgctccg gagtgatatc catgcccgcc atcacggctt ctacgcgacg gaatttcagg
    23461 cttgggatca ggctgtcaaa cgcctggtta gagaaagtgc aggttgcatc aatctcttta
    23521 cacagcgctt gtgccaggtc gacgtcaaaa ccaacgatct ggttgtttgc atcaatcgat
    23581 tcaaacggag gataggatgc ttcggtagca aaacgaatgg tttcggcagc tgtggcggaa
    23641 agactaaaac ctgcaattaa cgcggcaatc agaacttttt tcattgttgt tatcccgaat
    23701 cttagtgaga gagatagttt ttaaatgctt cggtttgcgg ctcggtaaag cagctcgcgt
    23761 cgccttgttc tacgatatga ccattttcca tatacaccac tcggctggcg gttttacgcg
    23821 ccacttcaac ttcgtgggtg acgatcacct gggtaatatt cgtttctgcc agctcacgaa
    23881 tgatgctgac gatttgtgcc gtaatttccg ggtccagtgc ggcggtcggt tcatcgaaca
    23941 gcagtacctg cggttccatc atcaacgcac gggcaatagc aacacgctgc tgctgaccac
    24001 cagaaagatg cagcgggtaa cgatcgctat aaggtttgag acgcagacgt tccagcagtt
    24061 tttctgcacg ggccagcgcc tgatctttac tcaaccccag tacacggcag ggcgcttcaa
    24121 tcaggttttg ctgcacggtc agatgcggcc acaggttgta ttgctgaaac accatgccaa
    24181 cgttacgacg caaatcgcga atcgctttgt cagagggtgt tttggtgaaa tcgaaatggt
    24241 tgcctgcaat gttgagcgta ccggagcgcg gcatctcaag cagattgagt acacgcagca
    24301 gcgagctttt acccgcgccg ctggggccaa gtaacaccag cgtttcgccc tgtgggcaat
    24361 ccagcgtgat atcgaacagc gcctgatgcg cgccgtagaa gcaattaatg ccgtttaatt
    24421 gaatactcat tgacactcgt atactggcag tctgatagct attgaggtcg aagatagtac
    24481 ctttgacaga ataattatgc aatatttctg ctttaaaagt taaaagcaaa gcgcattatt
    24541 caataaacat agcacaaaat aacgggggcg gtggtcggcg agcataaatg tcggcattcc
    24601 tcacgaaatg ccggacaatt tacggggttt attggttgat caaggcgtta gcgattctcg
    24661 atggactgac ggagcgtacc cgccgtggca tgaacgctgc cgcctaagta gcgtacatcg
    24721 tcgatcaccc aacactggcc ttcctgaatc attaacactt catcctgcca gctctgatca
    24781 ccctgtttta gatccacacg cagaggaata tttcgcgcat cacgattcgg gattgttgag
    24841 gcactggcaa cacgggcgct ctctggcaag gttgtgcgac tggagaatgg atcgttggtc
    24901 agtagttccc gatggttatt atcccgggag gcatcgctaa gcagtgtcgc cagtttgtcg
    24961 ctcagataag ggcgcaaggc ggtgatgtcg ttgctgcggt gcaaaatgcg gtagtcataa
    25021 aattgctggg ccacgttacc cgggcctcct tcaacgcaag gaccactgcg tgtgccgtta
    25081 tctttataag ctggagtgac tgtggtgcag gcactgagga gcagtgcgca ggggataagc
    25141 attgtcaatt tgctgtagcg cataatgatt tccttataag cgatcgctct gaaagcgttc
    25201 tacgataata atgatatcct ttcaataata gcgtatcagt ctgataatgc ttttgagatc
    25261 gaaggcttag caaacaagga gatcgatcat gcaattttct acaaccccaa ctctggaagg
    25321 ccagaccatc gttgaatatt gcggtgtggt gaccggcgaa gcgattttag gtgccaatat
    25381 tttccgtgat ttctttgccg gtatccgcga tatcgttggc ggacgttccg gtgcgtatga
    25441 aaaagaactg cgtaaagcac gggagatcgc ctttgaggaa ttaggctccc aggcgcgggc
    25501 gctgggggcc gatgccgtcg tcggtattga tatcgactac gaaacggtcg ggcaaaacgg
    25561 cagtatgctg atggttagcg tcagcggtac ggcggtgaaa acgcgtcgat gagaagagtc
    25621 ttctggctgg tcgctgccgc tctgttattg gcagggtgtg caggcgaaaa aggcattgtc
    25681 gagaaagagg gatatcagct tgatacccga cgccaggcgc aggcggcgta tccgcgcatt
    25741 aaagtgctgg tgatccacta caccgcagat gattttgata gctcgctggc gacactgacc
    25801 gataagcagg tcagctcgca ttatctggtc cctgcggtac caccgcgata caacggtaaa
    25861 ccgcgcatct ggcaactggt gccggaacaa gaactggcct ggcatgcggg gattagcgcc
    25921 tggcgcgggg caacgcgcct taacgacacc tctattggca ttgagctgga aaaccgtggc
    25981 tggcaaaaat cggccggagt gaaatatttt gccccgtttg aaccggcaca gattcaggcg
    26041 cttattccac tggcgaaaga tattattgcc cgttatcaca tcaagccgga aaacgtagtg
    26101 gcacatgcgg atatcgcacc gcagcgcaaa gacgatccgg ggccattatt tccctggcag
    26161 caactggcgc agcaggggat tggtgcctgg ccggatgcgc agcgggttaa cttttacctt
    26221 gccgggcgcg cgccgcacac accggtagat accgcgtcat tgctggagct tttggcgcgc
    26281 tacggttatg acgttaaacc tgatatgaca ccgcgcgagc agcggcgcgt gattatggca
    26341 ttccagatgc atttccgccc gacgttatat aacggcgaag cggatgcaga aactcaggcg
    26401 attgccgaag cattgctgga gaaatacggg caggattagc gcggcagttt tccgtggtcg
    26461 cgtagccagg cggcagtttt ctcgatacct tcatccaggg tgatgaccgg ctgataacct
    26521 aactcttcct gcgcacgcgt aatatccagc gtaaagtcaa aattcaactt ggagacgccg
    26581 tagtgggtca gcggcggctc ttttgctgac ttgcggaaac gctccatgct gcgggcgatc
    26641 atatccagca tcgggtaggg gacggaacga atacgacagt caatattcaa ctcgtcgatc
    26701 agcttctgca cgatgctgca cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc
    26761 ccggaaggta gcttatcgca ggcttcctgg cttgccagcc acattgcgtg cacggcattt
    26821 tcatagtagg tcatatcgac cagcgcactg ccgccatgcg gtaacagaat actgccgtag
    26881 tggtgcatca tatgcgccag acggggaata aagactttat cgtgcggtcc gaacagactt
    26941 tgtgggcgca gaatagtaaa gcgcgtttgt ggattcgcct gcgaaaacat attgatcact
    27001 tcttcgctgg ctgctttgct gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct
    27061 tctttaatat cgcgatggtg gtgataatcg aagtacaggg agggggaaga gatatgaata
    27121 aagttacgca caccccaggc gacagcccat tcacccaagc ggcgagtggc gcgaacgtta
    27181 gccagatcga aagcctgttg tgtcccccag ggtgaggtaa agctggagca gtgccacagc
    27241 gtatcaatgc ccgcgagcat cactttagct tgtgatgaaa ccagctcggt cagatccgcc
    27301 ggaacaaact ctgcgcccat tttttccagc aatttgccca ttgcctcgtt gcgaccggtc
    27361 gctcgcacgc tgatgccttt ctggcataaa aactctaccg cgtttcgacc taagccgctg
    27421 gtggcgccgg taaccagtac cttcatatca atccactgtt gttgagaaaa taacgtgcgc
    27481 attcttccgt gatttccccc atgatgcaat gggaaacatg aaagaataac gcaggttttg
    27541 tcgattaatc tgtgctttgt tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa
    27601 taaacagatg cgcggggatc atcaataacc agtaaaacag ccccggcata ccgtgcggat
    27661 gccagaaagc gcggacatcg atagtacgat agtcgccttt atcttccagg ctaaaacaca
    27721 gtcgtcccag ccccggcgct ttcatgccaa ataacaacgt aagttgtttt tccggttcaa
    27781 cgacaatcac tttccagcta tctaccgcat cgccagtctg taaatattcg cgctccgggc
    27841 ggcctttcgc cagcttatga ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa
    27901 tattgccaaa gaaataacgc tctttaccgc cgatttggtt cactacctgc cataaagcag
    27961 caaggctggc ggacgtttta acggtaaacc ccgcctgttt ggcaaaataa ccgtactccg
    28021 gtcgccagcg ggcaaaggcc tgagcgtcgt agccccagtc gctggagttg accagttttt
    28081 cctcctcttt caacgtgcta cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga
    28141 gtgcacgtag cgcggtatca tccgccagca gatcgtgttt cagcccctga atcaacgccc
    28201 tggcggtggt gggcggtacg gaagtaatca cattgagaaa ccacaccgaa atccagcggg
    28261 tggggagggg gatggggatc aaccagcggc gcttgccgct caccgccata aaatgttcaa
    28321 actgttgctg ataactgagc acctctggtc cggcggcttc gaagatgcgg tgttcgctgg
    28381 ccggatgatc taacaacgcc accagatagt gcagcaagtt ttccagcgcg atgggcgtgg
    28441 tgcgtgaacg tacccagcgt ggcggcgtta acactggcag gttgtagacc atatcgcgca
    28501 tgacttcgaa cgccgctgaa cctgcgccaa cgataattcc ggctcgaagt tcggtcacag
    28561 gtacattcgc ttcacgaaga atgtccgccg tagcctgacg agcacgcaga tgatccgact
    28621 gctcatgtgg cggggcctgc aacgaactga gaaagattaa ttgcttaact ggtacttcac
    28681 gtagcgcatc gcggacgttg agagccacct ggcgctcctg agcgataaaa tcgccgcctt
    28741 cgcccatgct gtgcaccaga aaatagacgg tatcgatatc ctgcaacagg gccggaaggt
    28801 tatccggcca gctgagatcg actttatggc aactgacgtt tgccagttgc agctttgcaa
    28861 gcctgtcgac atgacgtgcc gccgccagga tctgatgccc ttgctggctg agtgtgcgca
    28921 ccagatgctg accaatgtag ccactggcac cgagaactaa aatgcgttgc ggcacgtctc
    28981 tctccttaac gcgccaggaa tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc
    29041 gagacgtcaa gatgcgtcac caggcggaca atcggcgagg cgttaatcag cacgtttctc
    29101 actttcatgt attcgcctaa cgcggcagca ttttcttccc cgacgcgaac aaacagcata
    29161 ttagtgtcct gacgcatcac atccgcgcct gcttcacgca gttgctccgc catccaggcg
    29221 gcgttgtcgt gatcttcctg caaccgtgcg acgttatttt tcagggcata catcccggcg
    29281 gcagccagaa tcccggactg acgcatcccg ccaccggcca ttttccgcca gcggatggca
    29341 cgtttaatgt aatcacgatt accgacgagt aatgaaccga caggcgtccc aagacctttc
    29401 gacaggcaaa tggtgaacga atcacaatat tgcgtgatct ctttcagttc gcagccgtaa
    29461 gccaccacgg cattaaagat gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg
    29521 gtaaattccc atgcttcttt caggtattca cgcggcagca ctttgccgtt gtgggtgttt
    29581 tccagactga gtaatttggt gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc
    29641 actttatcca gcggtagcgt gccgtcggca gccgcgtcga tgggttgcgg ctgaatgctg
    29701 cccagcaccg ctgcgccacc ggcttcaaac agatagttat gcgcggcctg accgacaata
    29761 tactcttcgc cacgttcgca gtgactgagc agagcgacca ggttggcctg agtgccggta
    29821 ggcagaaaaa tggcggcttc tttaccggaa agctctgctg catagtcctg cagagcatta
    29881 acggtagggt cgtctccgta aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc
    29941 atggcgcggc tcggtcgggt aacggtatca ctgcgtaaat caatcatggc atgtccttat
    30001 tatgacggga aatgccaccc tttttacctt agccagttcg ttttcgccag ttcgatcact
    30061 tcatcgccgc gtccgctgat gattgcgcgc aacatataca agctaaatcc tttggcctgt
    30121 tcgagtttga tctgcggtgg aatggctaac tcttctttgg cgaccaccac atccaccagc
    30181 accggaccgt cgatggagaa ggcgcgttgc agggcttcat caacttcaga cgctttttct
    30241 acacggatac ccgtaatgcc gcacgcttcg gcaatgcggg caaagtttgt gtcgtgtagt
    30301 tcggtgccgt cagtcaaata gccaccagct ttcatctcca tcgccacaaa gcccagcacg
    30361 ctgttgttaa agacgacaat tttcactggc agtttcatct gcactactga gaggaaatcg
    30421 cccatcaaca tgctaaaacc gccatcgccg cacatggcga ccacctgacg ttctggctct
    30481 gtcgcctgcg cacccagcgc ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa
    30541 cctaacaggc gacgcttgcc gttcattttt agataacgtg ccgcccacac cgttggcgta
    30601 ccaacgtcac aggtgaaaat agcgtcatcg gcggcaaaat gactaatttg ctgcgccaga
    30661 tattgcgggt gaatggcttt ctcgctcggt ttagctaaat cgtccagccc tttgcgggcg
    30721 tcgcggtaat cttccagcgc tttatccaga aacttgcgat cggctttttc ttccaccaat
    30781 ggaagcaatg cacgcagagt cgacttgata tcgccgacca gtgccatatc caccttgctg
    30841 tgagcgccga tgctggctgg gttgatatca atctgaatga ttttggcatc ggtcgggtag
    30901 aaggcgcggt agggaaattg cgtgccgagt agcactaacg tgtcggcgtt catcatggta
    30961 tggaaacctg acgagaagcc gattaacccg gtcattccaa catcatacgg attatcgtat
    31021 tcgacatgtt ctttaccgcg cagggcatga acaataggcg ctttaatttt cccggcaaac
    31081 tcaactaact ctttatgcgc ccccgcgcag ccgctgccac acatcagggc gatattgctg
    31141 gaataacgca gcagttgcgc cagtttgcgt aactcttctt cttccggcgt cacgactggt
    31201 tgtggcgcat gataccagtg cgtggttgcc ccttctggcg caggttttaa cgccacgtcg
    31261 cctggtaaca cgacaaccga aacgccacgg ttaagcaccg ctttgcgcat ggcaatcgcc
    31321 agtacttgtg ggatctgctc cgggctggaa accagctcgc aatagtgact acattcgcgg
    31381 aatagctctt gtgggtgggt ttcctggaaa tagccgctgc caatttcgct ggagggaata
    31441 tgagcggcaa tcgccagtac cggaacgtga ttgcggtggc aatcaaacag gccgttgatt
    31501 aagtgcaggt tgccggggcc gcacgatccg gcgcagaccg ccagttctcc gctaagttgt
    31561 gcttcagcgc cagcggcaaa ggccgccact tcttcgtggc gggtggacat ccactcgatg
    31621 gtgcccatgc gattaagact gtcactaaga ccattcagag agtcgcctgt gactccccag
    31681 atgcgtttca cccctgccga ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc
    31741 atggttctcc atctcctgaa tgtgataacg gtaacaagtt tagttcatct gacggagggg
    31801 gaagggatgg gagagaaagg aggcactaac ggttaaatag cccgatgaaa ggaatatcat
    31861 cgggcataag gcgattatgc gagaaccaaa tccccctgcg gatggcagga gcaggccagt
    31921 acgtaacctt cagcgatttc ggcgtcggtc agcgtcattg tactgctcac cgtatattca
    31981 ccggaaacca cttttgtctt acagcagccg caaacacccg cacggcaggc agcgacaacc
    32041 ggaacgttat tgctttccag cgcctccagt agcgtggtgc caaccggggc gtaaaattct
    32101 cgtgccggtt gcagtttggt gaatttcaga ccgctggtcg ccgcttttgc tactggggtg
    32161 aagaatttct ctttaaagaa acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa
    32221 tccatatacg gagccgggcc gcaggtcatc acggtacgtg aagctaagtc aggtacacct
    32281 gccagcagtt ctcgagtgag acgaccagcg ataaagcctt cggtaacgtt attttctgcc
    32341 accagcgtta ccggatagtt acgccactca tcggcgaaaa taacatcctg cggcgtatgc
    32401 acgttgtaga tcacccgcac atcggcctgt ggacggttct tcgcaagcca gcgacgcatc
    32461 gacataatcg gcgtgacgcc gcagcctgcc gccagcaaca ggaatttatc ttctgcttta
    32521 tcgtcgcagg taaattcccc catcgcgtcc gaaagccaga gataatcacc gcgttttaca
    32581 tcgcgcgtca gccactggga gccgacaccg tcatcaatcc gccgcacggt cagggtgata
    32641 tattcactca cgcctggcgt ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt
    32701 cgcacgctga ccagtgcata ttgcccggcg cgatatgggt agtaatcgtg gcaaatcagg
    32761 gaaatcgtcc acacatccgg cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg
    32821 cattgattcg ttggcatcgt catcgacaaa ctccttacgc gctcaacagt tgcttcatgt
    32881 cttcttcaac agtggtgata gaacgcaggc cgaatttctc gttcagcacc gccagcaggt
    32941 ctggtgtcag gaaaccaggt gcagtcgggc cggtgacgat atttttcacg cccagagaaa
    33001 gcagcgtcag cagaatgacg atcgctttct gttcaaacca ggagagcacc agcgacagcg
    33061 gcagatcgtt cacaccacag cccagtttct ctgccagagt gacagccaga ataatcgctg
    33121 agtaagcatc gttacattga cctgcatcta ccagacgcgg cagaccttcg atatcgccaa
    33181 actcaagttt gttaaagcga tatttaccac aggcgagggt caggatcagg cagtcatccg
    33241 gcacgatggt ggcgaaatcg gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac
    33301 caagcaggaa gatatgacgc agtttttcac ggctcaccag atcaatcagc gtatcagcag
    33361 cgccaagcag cgtctggcga ccaaaaccca cggtgataag gtgcggaatt tcgctgtacg
    33421 ggaagcctgc catctgttgc gcctgggtga taaccgcaga gaaatcatca ccgtccagat
    33481 gacgcacgcc aggccagcca acaatgctgc gggtccagat acgatcgtca taagcgctta
    33541 cggttgggtc gatgatgcag ttcgaggtca tcacgatggg gccagggaaa cgagcgaact
    33601 ccacttgctg attctgccag ccgctgccgt agttaccgac cagatgcttg aatttacgca
    33661 gctccgggta gccatgcgca ggcagcattt cgccgtgggt gtagacatta acgcccgtgc
    33721 cttcggtctg ttccagcagg ttgtagagat ctttgagatc gtgaccggaa atcagaatgc
    33781 atttacccgc cgtcgctttg acgttgacct gggttggcgt cgggtgaccg tatttaccgg
    33841 tttcgcctgc atccagaatg ctcatcactt tgaagttcat ctggccgatt tccattgaac
    33901 actcaagaag cgcgttcata tcggcaggcc aggtccccag ccacgccatg attttatggt
    33961 actgggcata aatatcgttg tcgtattgac cgagaacatg cgcgtgttcc atataggccg
    34021 ccgcaccttt caggccatac aggcacagca gacgcaggcc gagaatgttt tcgccaatcg
    34081 ccgctttatc tttgttaggg gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat
    34141 cgctcaccag ttgcaggtca gccatcgggt tatcgacgcg cgcgttggca tctacagcca
    34201 ggcattgtgc tttcagcgcc tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac
    34261 gcggagaatc gaagttaacg ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt
    34321 caacatcgtg gttgatgatg ccgtattcac gcgcttttac cgcccaggca gaaagccctt
    34381 gcagcgccgc gatgagtaaa tcctgaaggt cagaagtttc cgccgtttta ccacacatcc
    34441 cctgcgcgta tgagcagccg tttcctgccg gagtacggat agtttgttca cattgcacac
    34501 aaaacatgat cacacctttt aaagttatat ttaatataca tgtttaaggt taagacgctt
    34561 aacgcgggga taaaagggat ttttcatgca actttaaggg agattgattt agcgcaattt
    34621 tggcggcagg gatctaccgc cagagaggta ttacgcagag aaaaaggcga tgaggatcgg
    34681 cactaacagg ctaagaataa aaccgtgaac aattgccgcc gggaccatat ccagcccgcc
    34741 agtacgttga agaacgggca gggtgaaatc cattgatgtg gcaccgcata agcccagtgc
    34801 agtagagcgg ctgcggcgaa tcagcccagg gatcaacata atagcaatca gttcacgggc
    34861 cagatcatta aaaaacgccg cgctcccgat taccggacca aaagattcgg tcaataaaat
    34921 accggaaaga gaataccagc cgaaaccgga ggccattgcc agcgcggtat tgatggggag
    34981 atcaagaata aaggcgttaa ttaaactacc aattaatgaa ctgacaacca ccaccacggc
    35041 gacaatcatt ccccggcgat taaggacaat ctgctttaag gtcatgccat tattgcgcaa
    35101 ctgaatacca acgaggaaaa gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg
    35161 ttgtaagaaa gccagtccac tcagaccaat ggcaaaacca atcactacta cgccgcacag
    35221 ttttagcgac tccagcgcca tcgcaatacg cgacgggagt ttttcttgct gatggtggtt
    35281 gcgccacggc aggcctcgct ccagccacat cagggcggca atattacaca gtaaaataac
    35341 ggtaatactg acggcagaat aatgcagaat cgccaacagg ttactggcga ggttatcgag
    35401 aaacgccaga ctgataccca taaaaaagag aataaggtaa accatccagc ttaatagctg
    35461 attaataact tttaacgcag cttgttggcg aagcggaatg aggtaaccca caatcagggg
    35521 aaccagaatg attaacagcc cagaaaacat gaaaacccag tccttgcaaa gatgaagtcg
    35581 aaatgcgcga tgacacacta ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa
    35641 atgttatgca taaggagcag tagagtattc gttttcattt aaagatattc ttgcgcttta
    35701 attacaaact gcaccgatgt tggtggcgtc aaaatcgccg aggcgttccc tgaaggccgg
    35761 ggcagcccac atggatgtgg gctgagggcg cgttttacag ggatgttacc tcgcgcccga
    35821 cccggtagcc gtaagggata agtcgagggc accgcgcagc ggcgattttg ttcgccagag
    35881 cccggggggg cagggggcgg cggcgattgg ccgccccctg cgcgctcctt gcgccagtgg
    35941 caatatgtgg cttagctcat gaaaggagcg caacaagatg atgaatcaac atataacaac
    36001 atcttaaaaa aagcctgaca ttacgccagg ccttctgcgt taattaatca cgcttttcca
    36061 gcagggtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
    36121 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
    36181 ggttaacaga agtgttagtc accggaatac taattaagtg aatcagggtt aaggccagac
    36241 caatagcgat cggcgcaaaa cctgccggcg cgaatttggc ggttgcgccg tggatcacca
    36301 acaggaaacc tgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
    36361 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
    36421 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
    36481 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
    36541 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcaaaggcc atcgtcagaa
    36601 cggtcagacc gaacgccaac gccacgccgg caaaaccaat gcctaattcc gggaagcctg
    36661 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
    36721 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa aaatatggtc
    36781 gttttatagg gtcgtcgtaa aaaaatgacg acggaaataa tgcgcggcta ttttaaaaac
    36841 gaaggcgagt cattcaccag ataaataaat ccagtaaatt tgatttaggg caacagcggg
    36901 ttgccccata tagtcatttg tctgattgac agtgtagtgc acgcaaaaga tttaatcctt
    36961 taggcgtaat aaaaaataat atatcatgct aattatttga ttttgttgtt tttgcagact
    37021 tatcagcaag agggagtata acgcgattat tcgctcattt ttcagacatt tgccatgctt
    37081 aaatgtgatg tcatcacgta ttagcaaggc ctttcccgtt atactgccag cgtaaaggat
    37141 aagtcacata tttctggagg ggatatgatt cttgagcgcg ttgaaattgt gggttttcgc
    37201 ggtatcaacc gtttgtcgtt gatgctggaa caaaacaacg tcctgattgg ggagaacgcg
    37261 tggggtaaat ccagcttgct ggacgcctta accctgctgc tatcgccaga atcagatctc
    37321 taccattttg agcgcgacga tttctggttc ccgccgggag atatcaacgg gcgagaacat
    37381 catctgcata ttattttgac cttccgcgaa tcgctgccag gccgacatcg ggttcgccgt
    37441 tatcggccgc tggaagcgtg ctggacgcca tgcaccgatg gctatcaccg tattttttat
    37501 cgtctggaag gggagagtgc ggaagacggc agcgtgatga cactgcgcag ttttctcgat
    37561 aaagacggac atccgattga tgtcgaggat attaacgatc aggcacgcca tctggtgcgt
    37621 ttaatgccgg tgctgcgctt gcgtgatgcc cgttttatgc gccgtattcg taacggcacg
    37681 gtgccaaatg tccctaatgt ggaagtcacc gcgcgccagc tcgatttcct cgcccgtgag
    37741 ttatcctcac atccgcaaaa tctctctgat gggcagattc gtcagggact ttccgcaatg
    37801 gtacagctgc ttgagcatta tttctctgag cagggggccg gacaggcgcg atatcgttta
    37861 atgcggcggc gggccagcaa tgagcaacga agctggcgct atctggacat catcaaccgg
    37921 atgattgacc gacctggagg gcgctcgtat cgggttattt tgctcggcct gtttgctact
    37981 ttattgcagg caaaaggcac attgagactg gataaagacg cgcgtccatt gttgctgatc
    38041 gaagatccag aaacccgttt acaccccatt atgctttcag ttgcctggca tctgttgaat
    38101 cttctgccat tgcagcgcat tgccaccacc aactcgggtg agttgctttc gttaacgccg
    38161 gtagagcatg tttgccgact ggtacgtgag tcctcgcgcg ttgccgcctg gcgtctgggg
    38221 ccgagtggct tgagtaccga agatagccga cgcatatcct ttcacattcg ttttaaccgt
    38281 ccgtcatcgc tgtttgcacg ctgctggttg ctggtggaag gggaaacgga aacctgggtt
    38341 atcaatgaac tggcgcgtca gtgcggacat cattttgatg ccgaagggat caaggtcatt
    38401 gagtttgccc agtccgggct aaagccactg gttaaatttg cccgccgaat ggggattgaa
    38461 tggcatgtac tggtcgatgg cgatgaagca gggaagaaat atgccgctac ggtacgcagc
    38521 ctgttgaata acgatcggga agccgaacga gaacatttaa cggcgttacc ggcgctggat
    38581 atggaacatt ttatgtatcg ccagggattt tccgatgtgt tccaccgcgt ggcgcaaatc
    38641 ccggaaaatg taccgatgaa tctacgcaaa attatctcga aagcgatcca tcgctcttcc
    38701 aaacccgatc ttgccattga agtggcaata gaggcaggac gtcgtggtgt ggactccgta
    38761 ccgacgctgc tgaaaaaaat gttctcacgc gtgctgtggc tggcgcgcgg tcgcgcggat
    38821 taaccgcgaa acatcgtggc catttgtggc tgaatagcgt cgagcatctc atagcgccga
    38881 cggtattcag cccgtttttt actggcgatt tcggcaatct cttttcgtgc tatctgtgct
    38941 ggaaggcggt aatggcgttc agcatcacat acgccgccaa ccgattccca gaaagcgttg
    39001 taatcagcgt ggatcttgcc ttctttatcg cgataacgca ggctgcggta aatatgcgtt
    39061 tcattgctga cggcaataat ctgctctacc tgcaaacgtt gggcaaacag acaggccgct
    39121 tccatcacga ggcgtttggg aaatagcccg tggcaggctt tcgtcgcatt ctggatttcc
    39181 tgatgtggaa tttcccattt tgcgccttgc agtccgccaa taaacatcgt tcttttcccc
    39241 tgatattcac acagggtaaa cgtgatctct gccagaggaa taccttcgct gttgcgaaac
    39301 aggattgtgc tgtcaccttc tttatccatt gagatcatca tggtcagctt aagcgtgaac
    39361 tgctcgccgt ttttgccttc cagcttcgcc agttgcagcc cgggggtatt caaatataag
    39421 ctgaattctt ccgccgacat acatccgcgg agtaacgcat aatggtaacg taacgcctcc
    39481 agcaattgct tacggctaag attcgccgca aggtaagggc gatgcagacg cacaggcagt
    39541 cgcggctggc gcgttaacaa cacattgaga ttaggccagt gggaaagttc gttcatccac
    39601 tcaacgctta aacgcggcat aatcaacgag cgcagcaaaa atttctggcg aaaactacgg
    39661 cgatgccaga atttacccgg ccgacactgt ccacgtgcca gactaagaaa aagtgacagg
    39721 ctgctgagag attcagatgg cgtaaaggtc cgttcagtta gctgcgacat attcatgaaa
    39781 tcattggtta tacatgacgt cgatttcacc attgcgtatc ttaaccaaac atcaatagtg
    39841 tgattactaa cgtaaatttt agggttttgt tgatatttcg ttgaagttaa tgacccggat
    39901 tggcatatgg agtattcaga atatttatga aaaagcggaa aaccgtgaag aagcgttacg
    39961 ttattgcgct ggtgatagtc atcgccggac tgattacgtt atggagaatt cttaacgcac
    40021 ccgtgccgac ttatcagaca ctgattgtgc gccccggtga tttacagcaa agcgtgctgg
    40081 cgaccggaaa gctggacgcg ctgcgtaagg ttgatgtggg cgcgcaggtc agcggtcagt
    40141 tgaaaactct gtcggtggcg attggcgata aagtaaaaaa agaccagctt ttaggggtta
    40201 ttgatcctga acaggctgaa aaccagatta aggaggtcga agcaacgctg atggagctac
    40261 gtgcgcagcg gcagcaggcg gaagcggagc tgaaactggc gcgggtgacg tattcccgtc
    40321 agcaacgtct ggcacaaacg caggcggttt cacagcagga tctcgacacc gccgcgacgg
    40381 agatggctgt gaaacaggcg caaattggca ccattgacgc ggaaatcaag cgcaatcagg
    40441 cttctctcga tacggctaaa accaatctcg attacactcg catcgttgcc ccgatggccg
    40501 gggaagtcac gcaaatcacc actctgcaag gccagacggt gattgccgca caacaagcac
    40561 cgaacattct gacgctggca gatatgagca ccatgctggt aaaagcgcag gtttctgaag
    40621 cggatgtaat ccacctgaag ccggggcaaa aagcttggtt tacggtgctt ggcgatccac
    40681 tgacgcgcta cgaggggcaa atcaaggatg tactaccgac gccggaaaag gttaacgacg
    40741 ctattttcta ttacgcccgt tttgaagtcc ccaaccccaa tggtttgctg cggctggata
    40801 tgactgcgca agtgcatatt cagctcaccg atgtgaaaaa tgtgctgacg atccctctgt
    40861 cggcgttagg cgatccggtt ggcgataatc gttataaagt caaattgttg cgtaatggtg
    40921 aaacacgcga gcgtgaagtg acgattggcg cacgtaacga taccgatgtt gagattgtca
    40981 aagggctgga agcgggcgat gaagtggtga ttggtgaggc caaaccagga gctgcacaat
    41041 gacgcctttg ctcgaattaa aggatattcg tcgcagctat cctgccggtg atgagcaggt
    41101 tgaggtgctg aagggcatca ccctcgatat ttatgccggt gagatggtag cgattgttgg
    41161 cgcttcaggt tccggtaaat cgaccctgat gaatattctc ggctgtctgg ataaggccac
    41221 cagcggcacc tatcgcgtcg ccggtcagga tgttgccaca ctggacgccg atgcgctggc
    41281 gcaactgcgc cgcgagcatt tcggctttat tttccagcgt taccatttac tttcgcattt
    41341 aaccgccgag cagaacgttg aagtacccgc cgtctatgcc ggtcttgagc ggaaacagcg
    41401 gctgcttcgc gcccaggagt tactgcaacg actggggctg gaagaccgta cagagtatta
    41461 tccggcacag ctttcgggtg gtcagcaaca gcgcgtcagc atcgcgcggg cattgatgaa
    41521 cggcggtcag gtaattcttg ccgatgaacc aaccggcgca ctggacagcc attctggcga
    41581 agaggtgatg gcgatcctgc atcagctgcg cgatcgcggg catatggtga ttatcgtcac
    41641 ccacgatccg caggtcgctg ctcaggccga gcgggtgatc gaaattcgcg acggcgaaat
    41701 tgtgcgcaat cctcccgcca ttgaaaaagt gaatgttgct ggcgggacgg agcctgttgt
    41761 caacacggtg tctggctggc ggcagtttgt cagcggtttt aacgaggcgc tgacgatggc
    41821 atggcgggcg ctggcagcga ataaaatgcg tactttactg accatgctgg ggattattat
    41881 cggtattgcg tcggtggttt ccattgtcgt ggtgggtgac gccgccaaac aaatggtgct
    41941 ggcggatatt cgttctattg gtacgaatac tattgatgtc tatcccggga aagattttgg
    42001 cgatgacgat ccgcaatatc agcaggcgct gaagtacgac gacttaatcg ccatccaaaa
    42061 acaaccgtgg gtcgcctcag ccacacctgc cgtctcgcaa aacctgcgcc tgcgttataa
    42121 caatgttgat gttgctgcca gtgccaatgg cgtgagcggc gattatttta atgtctatgg
    42181 catgaccttc agtgaaggaa acacctttaa tcaggagcag ctgaacggtc gtgcgcaggt
    42241 cgtggttctc gacagtaata ctcgccgcca gcttttcccc cataaagcag atgtggttgg
    42301 cgaggtgatt ctggtcggca atatgcccgc cagagtcatt ggtgtggcgg aagaaaaaca
    42361 gtcgatgttt ggtagcagta aagtgctgcg tgtctggcta ccttacagca cgatgtccgg
    42421 gcgagttatg ggccagtcgt ggctgaactc cattactgtc agggtaaaag aaggatttga
    42481 cagcgccgag gcggaacagc aactcacgcg tttactttca ctgcgccacg gaaagaagga
    42541 tttctttacc tggaacatgg acggcgtctt gaaaactgtt gaaaagacca cacgtacttt
    42601 acaactgttt ctgacgctgg tggcggtgat ttcgctggtg gtgggcggta ttggtgtaat
    42661 gaatattatg ctggtgtcag tgaccgagcg gacgcgggaa attggcattc gcatggctgt
    42721 aggtgcgcga gcaagcgatg ttttgcaaca gttcctgatc gaagccgtac tggtttgctt
    42781 ggtcggtggc gcgttgggaa taacgctgtc actgttaatt gctttcacct tgcagctttt
    42841 cttacccggc tgggagattg gtttttcacc gttggcactg ctgctggcgt ttctctgctc
    42901 gacggccacc gggattttat ttggatggtt acccgcacga aatgcggcac gactggatcc
    42961 agtagatgct ctggtacgag agtaattttt gagataaaaa tgccagccga tcgggctggc
    43021 attttgcctt taggatgtac acaatgagac agaagagcta tgcgactgcc gcttctactt
    43081 cgacgggcac aataacactg gcgtgattgc cttttggccc ctggtggaca tcaaactgaa
    43141 cggattgtcc agcttttagc gttctgtaac catccatctg aatggtggaa taatgagcga
    43201 aaatatcttc gccgccgcct tcagggcaga tgaaaccaaa ccctttggca ttgttgaacc
    43261 acttaacagt acccttttcc atgcttcgac atccttcgca aatcttatac aagtaagatg
    43321 gaataaaccg gggtcagaga gggggctgtt caaaacctcg ccaactctag aaatacaatt
    43381 tagagaatta gggcgagccg tcaagcgttt gacaggggcc agggggcagg tatgaatcaa
    43441 aaatttgaag cagttaacgc tattgacagg aatgtgacag atgtcgctga tgccaacgat
    43501 agatgatagt tatctatcat gtggagtaga ttggtcaggc aaataagctc ttgtcagcgg
    43561 cagggcgttc tgccgataac cgtaaccgaa gatgataact gacaatgggt aaaacgaacg
    43621 actggctgga ctttgatcaa ctggcggaag aaaaagttcg cgacgcgcta aaaccgccat
    43681 ctatgtataa agtgatatta gtcaatgatg attacactcc gatggagttt gttattgacg
    43741 tgttacaaaa attcttttct tatgatgtag aacgtgcaac gcaattgatg ctcgctgttc
    43801 actaccaggg gaaggccatt tgcggagtct ttaccgccga ggttgcagaa accaaagtgg
    43861 cgatggtgaa caagtacgcg agggagaatg agcatccatt gctgtgtacg ctagaaaaag
    43921 cctgaatgca ggcataaaaa ttgggggagg tgcctatgct caatcaagaa ctggaactca
    43981 gtttaaatat ggctttcgcc agagcgcgcg agcaccgtca tgagtttatg accgtcgagc
    44041 acttgttact ggcgctgctc agtaacccat ctgcccggga ggcgctggaa gcgtgttctg
    44101 tggatttggt tgcgctccgt caggaactgg aagcctttat tgaacaaacc acacccgttc
    44161 tgcctgccag tgaagaggag cgcgacacac agccgacgct gagttttcag cgtgtactgc
    44221 aacgtgcggt cttccatgtc cagtcctccg gtcgcaatga ggtaaccggt gcaaacgttc
    44281 tggtcgctat ctttagcgaa caggagtcgc aggcggcata tctgttgcgt aaacatgaag
    44341 tcagccgtct cgatgtggtg aactttatct ctcatggcac gcgtaaagac gagccgacac
    44401 agtcttctga tcctggcagc cagccaaaca gcgaagaaca agctggtggg gaggaacgta
    44461 tggagaattt cacgacgaac ctgaatcagc ttgcgcgcgt gggcggaatc gacccactga
    44521 ttggtcgtga gaaggagctg gagcgtgcta ttcaggttct ctgccgtcgc cgtaaaaaca
    44581 acccgctgct ggtgggggaa tctggtgtcg gtaaaaccgc gattgcggaa ggtcttgcct
    44641 ggcgaattgt tcagggcgat gtgccggaag tgatggctga ctgtacgatt tactctctcg
    44701 atatcggttc tctgttagcg ggcactaaat atcgcggcga ctttgaaaaa cgttttaaag
    44761 cgttgctcaa gcagctggag caggacacta acagcatcct gtttattgat gagatccaca
    44821 ccattatcgg tgcgggtgca gcgtctggtg gccaggtcga tgcggctaac ctgatcaaac
    44881 cgttgctctc cagcggtaaa attcgcgtaa ttggttcgac aacctatcag gagttcagca
    44941 acattttcga gaaagaccgt gctctggcgc gtcgcttcca gaaaattgat attactgaac
    45001 cgtcgatcga agaaactgtt caaatcatca atggcctgaa accgaagtat gaagcgcacc
    45061 acgacgtgcg ttataccgca aaagcggtgc gtgcagcggt agagctggcg gtgaaataca
    45121 ttaacgatcg tcatctgccg gataaagcca ttgacgttat cgacgaagcg ggcgctcgcg
    45181 cacgcctgat gccggtaagc aaacgcaaga aaaccgttaa tgtggcggat attgagtccg
    45241 tggtggcccg tattgcgcgc attccagaga agagtgtttc tcagagtgac cgcgataccc
    45301 tgaaaaacct cggcgatcgc ctgaaaatgc tggtcttcgg tcaggataaa gccattgagg
    45361 cgctgactga agccattaag atggcgcgtg caggtttagg tcacgaacat aaaccggttg
    45421 gttcgttcct gtttgccggc cctaccgggg tcgggaaaac agaggtgacg gtacagcttt
    45481 cgaaagcgtt gggcattgag ctgctgcgct ttgatatgtc cgagtatatg gaacgccata
    45541 ccgtcagccg tctgattggt gcgcctccgg gatacgttgg ttttgatcag ggaggtttgc
    45601 tgactgatgc ggtcatcaag catccacatg cggtgctgtt gctggacgaa atcgagaaag
    45661 cgcatccgga cgtgttcaat attctgttgc aggtgatgga caacggtacg ctgaccgata
    45721 acaacggacg caaagcggac ttccgtaacg tggtgctggt gatgaccacc aacgccgggg
    45781 tacgtgaaac tgagcgtaaa tccattggtc ttatccacca ggataacagc accgatgcga
    45841 tggaagagat caagaagatc tttacaccgg aattccgtaa ccgtctcgac aacattatct
    45901 ggttcgatca tctgtcaact gacgtgatcc atcaggtggt ggataaattc atcgtcgagt
    45961 tgcaggttca gctggatcag aaaggtgttt ctctggaagt gagccaggaa gcgcgtaact
    46021 ggctggccga gaaaggttac gaccgggcaa tgggcgcacg tccgatggcg cgtgtcatcc
    46081 aggacaacct gaaaaaaccg ctcgccaacg aactgctgtt tggttcgctg gtggacggcg
    46141 gtcaggtcac cgtcgcgctg gataaagaga aaaatgagct gacttacgga ttccagagtg
    46201 cacaaaagca caaggcggaa gcagcgcatt aatctgattg ttaggtaggt tggtcaagtc
    46261 cgtaatctcg aaagaggtta cggacttttt gtttatgggg tggaggaggt tcagaccctt
    46321 tttttaatga tgatggtaag ttgttgataa ttagtgctgc gggaaggtaa ggataaaaaa
    46381 gggtgctgca ggagaatggg atggttttgc tttatttaca acgggctaaa cgtgtagtat
    46441 ttgagttcac tgccgtacag gcagcttaga aattcacagg taacatactc cacccgccca
    46501 ccatgttcac tgccgtacag acagataaaa tgcgaaaaaa aagctcgcac tttcgtacga
    46561 gctcttcttt aaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccgtatac
    46621 acactttcca ggcgtgctcc ttcagccact cggacacctc accaaattgt tttgctacca
    46681 aacctcatgg gtggcaacgg ggcgctacta tagggagttg gagtaaaacg gtcaagaaga
    46741 attttaatga taattattgt ttgctcatac tgtaaacaag ttgtgcagta tatctacatc
    46801 gagacaagtt acggacttat acttccaaag tacttcatac atatcacaaa ataaaaaggc
    46861 cggttaaacc gaccttttac tcgttctttc tcttcgccca tcaggcggta aaacaatcag
    46921 cgactacgga agacaatgcg gcctttgctc aggtcgtacg gggtcagttc aacagtcact
    46981 ttgtcgcccg tcaggatgcg gatgtagttt ttgcgcattt taccggagat gtgtgcagta
    47041 accacgtgac cgttttctaa ctctacgcgg aacatggtat taggcaacgt ttcaagaacg
    47101 gtaccttgca tttcaatatt gtcttctttg gccatctaat cctctggggt atcactaccg
    47161 taatttgaac cggcaagata atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa
    47221 atcgcaacaa acaggttcgg cacattactc cgaaaacaca cggctaagcc gcaccaaaag
    47281 cgcaacgtat aagggagcgg tgagataaac gatgggcgtt acctgacgcg aaaaattcct
    47341 tatcggcagc ggggtaatga gcgtaaccaa ctctgcgacc gcaattataa cactctgggg
    47401 agaaatgtgc cgaaaacatt cattcttgtg gtgaaaacaa gcatcgtggt acccagaaat
    47461 tattcggcaa tcgtccgagg cgcatttgat tgagataatt aaggtaatcc cggcggggaa
    47521 tttcgcaggc accaagcgat gctgtgtgat cgttaaggac ctggcagtcg ataagcttac
    47581 cgccatgacc gataaattcc tcacagaata ccagaagcgc cgttttagac gcattttcca
    47641 tccggctgaa catggactcg ccacaaaata gcgttccctg ggccacgccg tacataccgc
    47701 cgacaagctc atcttcacgc cagacttcaa tggagtgggc atggccgagt tcgtgaaggc
    47761 gatggtaggc ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac
    47821 agccttcaat gacctgacca aaagcgtaat tcatcgtgac acgatagggc gagcgtttat
    47881 gaaatcgctt catactacgg ctgatatgca gtgattctgg ccatagcacc gcgcggggat
    47941 cgggcgacca ccagaggatg gggtcgcctg gagaaaacca cggaaaaata ccacgctggt
    48001 aagccattaa caggcgcgca gggctaagat cgcccccaag tgccagcagg ccgttaggct
    48061 cacgtaatgc gccttccggg gaagggaagg ctattgaatg gcgagaaagc tgaaccaagc
    48121 gcatgaccgc aaaactccac gcaagtcgga tcgttcaata atagcttaca aaccctgctt
    48181 gaactggtaa taacgcccct gtctggcaag cagctctgcg tgagtacctt gctcaataat
    48241 ttgcccgttg tccatcacta ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt
    48301 gaccattaac accgttttct cacgcatcat ttctgcaagc aattcaagga tctggctttc
    48361 ggttgtggca tctaagcctt cggtaggttc atccagcaac accagtggcg catcatgtaa
    48421 cagcgcacgg gcgatagcca gacggcgcag ttcaccaccg gagagctggc gtccgccttc
    48481 acctaaccaa ctgttgagac ctgcatcctc gagcagcttc tccagaccaa cgcgacgcaa
    48541 gctctccgcc agagcctcat cactactgcc aggcgaggcg agtaacagat tatcacgcag
    48601 cgtggcgcta aacagatgca ctcgctgagg aacaacgctg atggtctgtc gtagagccgc
    48661 ttcattcagg ctggctatgg ggctatcgtt aagcaaaatc tcgccctgtt gcgggtccca
    48721 agcgcgggtc agcagttgta acagtgttga tttgccgcat ccggttcgcc cgagaatcgc
    48781 aatatgttcc ccggcgttta cctgaagaga aatcccttta agtgcctgtt gggattgctc
    48841 cggataagtg aactgaacat cccgtaatgt cagcgaaacg cgatcggcaa cacgagtttg
    48901 ggtatcaggg aaggtgactt ccggtttttg atccgttaaa tcagtgatcc gaacggcaga
    48961 ggcaatgact tgccccagat gctgaaatgc acccgttact ggtgccagtg cttcaaacgc
    49021 ggctaacgcg cagaagacaa acagggcaat taacgcgccg ggttgagcat tgccgccaac
    49081 gccgccagac gccatccaca gcatcaggat caccgctaac gcgccaatga gcagcattat
    49141 cgcttgcgac aatgcggtca gttcagattg acggcgttgc gcttccagcc attgaatttc
    49201 tgtattctct agttgcgtgc gataacgatc gctggcacca aaaatggtca gctcagcttg
    49261 cccttgcagc caggccgtca gttgttggcg atactgtccg cgaagatgag tcagattttg
    49321 cccggtgctt tttcccgcac gataaaacag cggtggcatc aggaaaagcg tcagtaacat
    49381 aatgccgccc agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga ttgtcaccat
    49441 cataatcacc acaaaagcgc ccaccagcgg cgagataacg cgcaggtaaa gatgatcgag
    49501 cgtatcaaca tccgccacca cgcgattgag caattcgccc tgacgatagc gcgccagtcc
    49561 ggcaggggag aggggcaaca atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg
    49621 gaaagtcgcg tcgtgactta ccagacgttc aaaataacgc ccggcagtac gggtgattgc
    49681 ggcaccacgc acgcccgcag cgggtagcat atagttgaag ctgtacagtc cggcaacccc
    49741 cgcaaccgct gaggccgaga ggaaccagcc ggaaagtgtc aacagtccga tactggcgag
    49801 cagcgtcaca attgccagca caataccaag acttaacatc catttatgac gtttatacag
    49861 tgctagatag ggtagcaaag cgcgcattta aatctcctcc tgacgatggg ccagtaatgt
    49921 ggcgaatggg ccaccagcca cacttaattc cgcgtaacgt ccttgctcaa taatccggcc
    49981 atcctgcata acccaaatga catcccagtc agcaagatct tctaactggt gggtgaccat
    50041 taacgttgtc tggcgcagag aggcggcatt cagcgcctcc attacgcgct gttcactgtg
    50101 agcatcaagg ctggcagcgg gttcatccaa cagtaatagc gaacagggat ttagtaacgc
    50161 acgggccacc gccacgcgct gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac
    50221 aggcgtatca acgccttgtg gcaggagcgg tagaaactcg ctgacccagg cgttatccag
    50281 cgctgcttgt aattcatgtt cgctggcatc aggtcgcgcc agtagtacgt tatcccgtaa
    50341 tgttgctgcc ggtaattgtg gattttgccc aacccaggag agatgtttac gccatgattc
    50401 tggtgataaa tcgcgtaatt ctatcccgtt gattcgtagc gatccctgat atgagagaaa
    50461 accagaaagc gcgttcaaca gtgaactttt acctgaaccg ctgcgaccaa ccaacacggc
    50521 acgttggcct gctggcaaag taaagttcag cggtccggcc agcgttttac cttccggcga
    50581 cgtgataaac agatcctcgg cctcaatggt caccggatcg gtcgatgcta attccacctc
    50641 gccacactgc ggatgggcga gcggggtttc cataaacgtt ttcagactgt cagctgcacc
    50701 aacagcctgg gctttagcat gataaaacgt accgagatcg cgtaatggct ggaaaaactc
    50761 tggcgcaagg atcagggcca gaaaacccgc agccagcgtc acgccagtat cgtagtgacc
    50821 aaaatccagc tcgccaagat aggaaaaacc aaagtagacc gccaccagag caattgacag
    50881 cgaggtaaaa aattcgagaa tgccggagga taaaaatgcc agccgtagca cttccattgt
    50941 ccgttggcgg aaatcttccg aagcagaacg aatactttca atttcagctt caccacgacc
    51001 aaaaatacgc aatgtttcca tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc
    51061 aagagcgaga aagttacgtc ggttagcatc ggcagccccc attccaacca gcgccataaa
    51121 caacggaatt aacggtgcag tgcccagcag aatgagcgcc gcagcccagt ttgaggggaa
    51181 gattgccacc acaatcagca acggcaccga cactgccagc gccatttgcg gcaggtagcg
    51241 tgcatagtaa tcatgcatat cgtcaatttg ctcgagtacc agcgtcgccc agctccccgc
    51301 aggtttaccc tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa cctgccggcg
    51361 gatggcaaag cggatatgct gcccggcgtg ataacccacc cgttcgcgta accagaccac
    51421 ccatgcgcgc agtacaaagg tcagaaccag taacgtaaag ggaagcagca gggcttcacg
    51481 gggaatattc tccataatca tatgttgcag aattcgcgcc atgaaccagg cctgggcaat
    51541 gatcaatatg ccgctcacaa agcccagcag acgagaaata ttcagccaac gttgggagat
    51601 gacgctttgc tgttttaacc agcgggttaa ctctttttga cgagatttat tcattgcacg
    51661 cttagcaggt gagttatcag aattatttgc agagcaatgt tacaacgggg aaaaaataaa
    51721 ggcgacccat agtcgcaagg tgtcgccttc tttacttttg ttactgattt gtaaaattat
    51781 tttgcgtcag ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat gcagcctgta
    51841 ccggccgaag taatggcctg acgataaata tgatccatca cgtcacccgc ggcaaagacg
    51901 ccaggaatgc tggtctgggt ggcattacca tgaatacccg actgtacttt gatgtagccg
    51961 ttttccagtt ccagctgccc ttcgaaaatg gcagtattcg ggctgtgacc gatagcaaca
    52021 aacagaccgg caacgtcgag tgattcgatg ttatcgctgt tttgcgtatc gcgcagacga
    52081 acgccagtga cacccatttg atcgccggtc acttcttcca gcgtccggtt ggtgtgcaga
    52141 atgatgttgc cgttctccac tttatccatc agacgcttaa tgaggatttt ttccgcgcgg
    52201 aaaccgtcac ggcggtgaat cagatgtact tccgaagcga tgttagacag atacaacgcc
    52261 tcttcaaccg cggtattgcc gccgccgatg accgcaactt tctggttgcg atagaagaaa
    52321 ccgtcgcagg ttgcacaagc agaaacccca cggcctttaa aggcttcttc agagggcagg
    52381 ccgagatagc gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt gtattcgccg
    52441 ttatcgccat tcagacggaa cggacggttt tgcagatcca ccttgttgat atgatcaaaa
    52501 atgatttcag tttcaaattt ggtggcatgt tcgtgcatgc gctccattaa taacggaccg
    52561 gtcagatcgt ttggatcgcc aggccagttt tccacttctg tggtggtggt cagttggccg
    52621 cctttttcca tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc atagacagca
    52681 gcggtgtatc ccgccgggcc tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc
    52741 atgagatccc catagttgtt ggcagacaat gggcaggatt gtagggaatt tactgacgta
    52801 aaaaaagagt atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt
    52861 caaattctcc cgtcattatc acctctgcta cttaaatttc ccgctttata agctgattaa
    52921 atgatgaata aacgcccctg ttaatgaata tctggcatgt tgtactaaaa atcgatgttt
    52981 tgctttgaca atcccctggt gttttgcgaa aacattcgag gaagaaaaaa aacagtattc
    53041 ttatatgcgc ataaccatgc atgtaaatac catgtttacc gtgctagtga aatctacgta
    53101 tggcgtggac agacgccatt cgtgatgtcg atagctgcca caaggcaacg gtcttctcac
    53161 cgtagaccca ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg tgacgggtag
    53221 cgactctgaa cagtgatgtt tcagggtcag acaggagtag ggaaggaata cagagagaca
    53281 ataataatgg tagatagcaa gaagcgccct ggcaaagatc tcgaccgtat cgatcgtaac
    53341 attcttaatg agttgcaaaa ggatgggcgt atttctaacg tcgagctttc taaacgtgtg
    53401 ggactttccc caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca agggtttatt
    53461 cagggctata cggcgctgct taacccccat tatctggatg catcacttct ggtattcgtt
    53521 gagattactc tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac cgctgtacaa
    53581 aaacttgaag aaattcagga gtgtcattta gtatctggtg atttcgacta cctgttgaaa
    53641 acacgcgtgc cggatatgtc agcttaccgt aagttgctgg gggaaaccct gctgcgtctg
    53701 cctggcgtca atgacacacg gacatacgtt gttatggaag aagtcaagca gagtaatcgt
    53761 ctggttatta agacgcgcta acacggaaca ggtgcaaaat cggcgtattt tgattacact
    53821 cctgttaatc catacagcaa cagtactggg gtaacctggt actgttgtcc gttttagcat
    53881 cgggcaggaa aagcctgtaa cctggagagc ctttcttgag ccaggaatac actgaagaca
    53941 aagaagtcac attgacaaag ttaagtagcg gccgtcgcct tctggaagcg ttgctgatcc
    54001 ttattgtcct gtttgccgtc tggttgatgg ctgccttact aagctttaac ccttcggacc
    54061 ccagctggtc gcaaacggcc tggcatgaac ctatccataa tttaggtggg atgcccggtg
    54121 cgtggctggc agatacgctg ttctttattt ttggcgtgat ggcttacacc attcccgtca
    54181 ttattgtcgg cggttgttgg tttgcctggc gtcatcagtc cagtgacgaa tacattgatt
    54241 attttgccgt ttcgttacgc atcattggcg ttttggcgct catccttacc tcctgtggtc
    54301 tggcggcaat caacgctgac gatatctggt attttgcctc cggtggcgtc attggcagct
    54361 tactaagcac tacgttacaa ccactgctac acagtagcgg gggaactatt gcgctgctct
    54421 gcgtttgggc tgcgggcctg acgctgttca ccggttggtc atgggtgacc attgctgaaa
    54481 aactcggcgg ctggatttta aacattctca ccttcgccag taatcgtacc cgtcgcgatg
    54541 atacctgggt cgatgaagat gaatatgaag atgacgaaga gtatgaagat gaaaaccacg
    54601 gcaaacagca tgaatcacgc cgtgcccgta ttcttcgcgg cgcgctagcg cgtcgtaaac
    54661 ggttggcgga aaaattcatt aatccgatgg ggcggcaaac agacgctgcg ttgttctccg
    54721 gtaagcggat ggatgatgaa gaagagatta tctacactgc acgcggtgtg gctgccgacc
    54781 cggacgacgt cctattttcg ggcaatcgtg caacgcagcc agaatatgac gaatacgatc
    54841 cattattaaa cggtgcgcca attaccgaac ctgttgctgt agcagctgct gctaccacgg
    54901 cgacacaaag ctgggctgcg ccggttgaac ctgtgaccca gacgccgcct gttacttctg
    54961 ttgatgttcc accttcgcaa cctacagtag cctggcagcc tgtaccgggt ccacaaacgg
    55021 gagagccggt tattgctcct gcaccggaag gttacccaca gcagccacaa tatgcgcagc
    55081 ctgcagtgca atataatgag ccgctgcaac aacctgtaca gccacagcag ccgtattatg
    55141 cgcctgcagc tgaacaacct gtgcaacaac cttactacgc tcctgcggcg gaacaacctg
    55201 ttcagcaacc gtattacgca actgcgccgg aacagcctgc gcaacagccg tattatgccc
    55261 ctgcgccaga acaaccggtg gcaggtaacg cctggcaagc cgaagagcag caatccactt
    55321 ttgctccaca gtctacatac cagactgagc aaacttatca gcagccagcc gctcaggagc
    55381 cgttgtacca acagccgcaa cccgttgaac agcagcctgt tgtggagcct gaacccgttg
    55441 tagaagagac aaaacccgcg cgtccgccgc tttactattt tgaagaagtg gaagagaagc
    55501 gagcccgtga acgtgaacaa cttgcggcct ggtatcaacc gattccagaa ccggttaaag
    55561 aaccagaacc gatcaaatct tcgttgaaag cgccttctgc tgcagcagta cctccagtag
    55621 aagccgctgc cgctgtttct ccgctggcat ctggcgtgaa aaaagcgaca ctggcgacgg
    55681 gggctgccgc aaccgttgcc gcgccagtct tcagtctggc aaatagcggt ggaccgcgtc
    55741 cgcaagtcaa agagggaatt ggcccgcagt tgccacgacc gaaacgtatt cgcgtgccaa
    55801 ctcgtcgtga actggcgtct tacggtatta agctgccctc acagcgtgcg gcggaagaga
    55861 aagcccgtga agcccggcgc aatcagtatg attctggcga tcagtataac gatgatgaaa
    55921 tcgatgcgat gcagcaggat gaactggcac gtcagttcgc ccagacacag cagcaacgct
    55981 atggcgaaca gtatcaacat gatgtgcccg taaacgcaga agatgcagat gctgcggcag
    56041 aggctgaact ggctcgtcag tttgcgcaaa ctcaacaaca acgttattcc ggcgaacaac
    56101 cggctggggc gaatccgttc tcgctggatg attttgaatt ttcgccaatg aaagcgttgc
    56161 tggatgatgg tccacacgaa ccgttgttta cgccaattgt tgaacctgta cagcagccgc
    56221 aacaactggt tgcaccgcag cagcagtatc agcagccaca acagcaggtt gcgccacagc
    56281 agcaatatca gcagccgcaa cagccggttg cgccgcagcc gcagtatcag cagccacaac
    56341 agcaggttgc gccgcagcca caatatcagc agccgcaaca accggttgca ccgcagcagc
    56401 aggataccct gcttcatccg ctgttgatgc gtaatggcga cagccgtccg ttgcataaac
    56461 cgacgacgcc gctgccttct ctggatttgc tgacaccgcc gccgagcgaa gtggagccgg
    56521 tagatacctt tgcgcttgaa caaatggctc gcctggtgga agcgcgtctg gctgatttcc
    56581 gtattaaagc cgatgtcgtc aattactctc cggggccggt tatcactcgc tttgaactta
    56641 acctggctcc tggcgtaaaa gcggcgcgca tttctaactt gtcacgggac cttgcccgtt
    56701 cactttcgac ggtggcggtg cgtgtcgttg aagttattcc tggcaaaccc tatgtaggtc
    56761 tggagttacc aaataaaaaa cgacaaaccg tttatctgcg cgaagttttg gataacgcca
    56821 aattccgcga taatccgtcg ccattaaccg tggtgctggg taaagatatc gccggtgagc
    56881 cggtggttgc cgatctggcg aaaatgccgc acttgttggt tgcggggact accggttccg
    56941 gtaaatctgt cggtgtgaac gcgatgatcc tgagcatgct ttataaagca cagccagaag
    57001 atgtgcgttt catcatgatc gacccgaaaa tgctggagct ttcggtttat gaaggcattc
    57061 cgcatctgtt aacggaagtc gttactgata tgaaagatgc cgccaacgcg ctgcgctggt
    57121 gtgttaacga gatggagcgt cgttataaac tgatgtctgc gctgggtgtg cgtaatctgg
    57181 cgggttataa cgaaaaaatt gctgaagccg atcgcatgat gcgtccgatt ccagacccgt
    57241 actggaagcc gggtgacagt atggatgccc agcatccggt gctgaaaaaa gaaccataca
    57301 ttgtggtgtt ggttgacgaa tttgccgacc tgatgatgac ggtaggtaaa aaagtggaag
    57361 agctgatagc acgtctggcg caaaaagccc gtgccgcggg tatccacctc gtactggcaa
    57421 ctcagcgtcc atcggttgat gttattactg gtctgattaa agcgaatatt ccgacccgta
    57481 tcgcctttac cgtatccagt aagattgact cacgtaccat tcttgatcag gctggcgcgg
    57541 aatcactgct ggggatgggg gatatgctct actctgggcc gaactccacg ttgccggtac
    57601 gtgtccatgg tgcttttgtt cgcgatcagg aagttcatgc cgtggtgcag gactggaaag
    57661 cgcgtggtcg cccacagtat gttgatggca tcacttccga cagtgaaagc gaaggtggcg
    57721 cgggcggttt cgatggcgct gaagaactgg atccgttgtt cgatcaggcg gtgcagtttg
    57781 tcactgaaaa acgcaaagcg tcaatttctg gcgtacagcg tcagttccgc attggttata
    57841 accgtgcagc gcgtattatc gaacagatgg aagcgcaggg gattgtcagc gaacaggggc
    57901 acaacggtaa tcgtgaagtg ctggccccac cgccgtttga ctaattaatg cattgccgga
    57961 taaggcgcgg tagcgtcgca tccggcactc tatcaactga aaattcagta ttttcttttt
    58021 tcctcaagct gattattagc ctggaataga gagtagaggg aactcccgat cgggagtgac
    58081 gtaatttgag gaataatgat gaaaaaaatt gccatcacct gtgcattact ctcaagctta
    58141 gtagcaagca gcgtttgggc tgatgccgca agcgatctga aaagccgcct ggataaagtc
    58201 agcagcttcc acgccagctt cacacaaaaa gtgactgacg gtagcggtgc ggcggtgcag
    58261 gaaggtcagg gcgatctgtg ggtgaaacgt ccaaacttat tcaactggca tatgacacaa
    58321 cctgatgaaa gcattctggt ttctgacggt aaaacactgt ggttctataa cccgttcgtt
    58381 gagcaagcta cggcaacttg gctgaaagat gccaccggta atacgccgtt tatgctgatt
    58441 gcccgcaacc agtccagcga ctggcagcag tacaatatca aacagaatgg cgatgacttt
    58501 gtcctgacgc cgaaagccag caatggcaat ctgaagcagt tcaccattaa cgtgggacgt
    58561 gatggcacaa tccatcagtt tagcgcggtg gagcaggacg atcagcgcag cagttatcaa
    58621 ctgaaatccc agcaaaatgg ggctgtggat gcagcgaaat ttaccttcac cccgccgcaa
    58681 ggcgtcacgg tagatgatca acgtaagtag aggcacctga gtgagcaatc tgtcgctcga
    58741 tttttcggat aatacttttc aacctctggc cgcgcgtatg cggccagaaa atttagcaca
    58801 gtatatcggc cagcaacatt tgctggctgc ggggaagccg ttgccgcgcg ctatcgaagc
    58861 cgggcatctg cattctatga tcctctgggg gccaccgggt accggcaaaa caactctcgc
    58921 tgaagtgatt gcccgctatg cgaacgctga tgtggaacgt atttctgccg tcacctctgg
    58981 cgtgaaagag attcgcgagg cgatcgagcg cgcccggcaa aaccgcaatg caggtcgccg
    59041 cactattctt tttgttgacg aagttcaccg tttcaacaaa agccagcagg atgcatttct
    59101 gccacatatt gaagacggca ccatcacttt tattggcgca accactgaaa acccgtcgtt
    59161 tgagcttaat tcggcactgc tttcccgtgc ccgtgtctat ctgttgaaat ccctgagtac
    59221 agaggatatt gagcaagtac taactcaggc gatggaagac aaaacccgtg gctatggtgg
    59281 tcaggatatt gttctgccag atgaaacacg acgcgccatt gctgaactgg tgaatggcga
    59341 cgcgcgccgg gcgttaaata cgctggaaat gatggcggat atggccgaag tcgatgatag
    59401 cagtaagcgg gtcctgaagc ctgaattact gaccgaaatc gcaggtgaac gtagtgcccg
    59461 ctttgataac aaaggtgatc gcttttacga tctcatttcc gcactgcata aatcggtacg
    59521 tggtagcgca cccgatgcgg cgctgtactg gtatgcgcga attattactg ccggtggcga
    59581 tccgttatat gttgcgcgtc gctgtctggc gattgcgtct gaagatgtgg gtaatgctga
    59641 tccacgagcg atgcaggtgg caattgcagc ctgggattgc tttactcgcg ttggcccggc
    59701 ggaaggtgaa cgcgccattg ctcaggcgat tgtttacctg gcctgcgcgc caaaaagcaa
    59761 cgctgtctac actgcgttta aagctgcgct ggcggatgct cgcgaacgtc cggattatga
    59821 cgtgccggtt catttgcgta atgcgccgac gaaattaatg aaggaaatgg gctacgggca
    59881 ggaatatcgt tacgctcatg atgaagcaaa cgcttatgct gccggtgagg tttacttccc
    59941 gcgggaaata gcacaaacac gctattattt cccgacaaac aggggccttg aaggcaagat
    60001 tggcgaaaag ctcgcctggc tggctgaaca ggatcaaaat agccccataa aacgctaccg
    60061 ttaatgttat cgttgcggta atgttgttac tgtatccctg tggtcgcagg ctgtggccac
    60121 atctcccatt taattcgata agcacaggat aagcatgctc gatcccaatc tgctgcgtaa
    60181 tgagccagac gcagtcgctg aaaaactggc acgccggggc tttaagctgg atgtagataa
    60241 gctgggcgct ctcgaagagc gtcgtaaagt attgcaggtc aaaacggaaa acctgcaagc
    60301 ggagcgtaac tcccgatcga aatccattgg ccaggcgaaa gcgcgcgggg aagatatcga
    60361 gcctttacgt ctggaagtga acaaactggg cgaagagctg gatgcagcaa aagccgagct
    60421 ggatgcttta caggctgaaa ttcgcgatat cgcgctgacc atccctaacc tgcctgcaga
    60481 tgaagtgccg gtaggtaaag acgaaaatga caacgttgaa gtcagccgct ggggtacccc
    60541 gcgtgagttt gactttgaag ttcgtgacca cgtgacgctg ggtgaaatgc actctggcct
    60601 cgactttgca gctgcagtta agctgactgg ttcccgcttt gtggtaatga aagggcagat
    60661 tgctcgcatg caccgcgcac tgtcgcagtt tatgctggat ctgcataccg aacagcatgg
    60721 ctacagtgag aactatgttc cgtacctggt taaccaggac acgctgtacg gtacgggtca
    60781 gctgccgaaa tttgctggcg atctgttcca tactcgtccg ctggaagaag aagcagacac
    60841 cagtaactat gcgctgatcc caacggcaga agttccgctg actaacctgg tgcgcggtga
    60901 aatcatcgat gaagatgatc tgccaattaa gatgaccgcc cacaccccat gcttccgttc
    60961 tgaagccggt tcatatggtc gtgacacccg tggtctgatc cgtatgcacc agttcgacaa
    61021 agttgaaatg gtgcagatcg tgcgcccgga agactcaatg gcggcgctgg aagagatgac
    61081 tggtcatgca gaaaaagtcc tgcagctgct gggcctgccg taccgtaaaa tcatcctttg
    61141 taccggcgac atgggctttg gcgcttgcaa aacctatgac ctggaagtat ggatcccggc
    61201 acagaacacc taccgtgaga tctcttcctg ctccaacgtt tgggatttcc aggcacgtcg
    61261 tatgcaggca cgttgccgca gcaagtcgga caagaaaacc cgtctggttc ataccctgaa
    61321 cggttctggt ctggctgttg gtcgtacgct ggttgcagta atggaaaact atcagcaggc
    61381 tgatggtcgt attgaagtac cagaagttct gcgtccgtat atgaacggac tggaatatat
    61441 tggctaatac ccaatttttc tgaatctaaa aagcgcctgc gggcgctttt tttgtctccc
    61501 tttgataccg aacaataatt actcctcact tacacgtaat actactttcg agtgaaaatc
    61561 tacctatctc tttgattttc aaattattct atgtatacaa gcctatatag cgagctgcta
    61621 tagaaataat tacacaatac ggtttgttac tggaatcaat cgtgagcaag cttgagtgag
    61681 ccattatgaa aacgaaaatc cctgatgcgg tattggctgc tgaggtgagt cgccgtggtt
    61741 tggtaaaaac gacagcgatc ggcggcctgg caatggccag cagcgcatta acattacctt
    61801 ttagtcggat tgcgcacgct gtcgatagcg ccattccaac aaaatcagac gaaaaggtta
    61861 tctggagcgc ctgtacagtt aactgtggta gtcgctgccc gctacgtatg cacgtcgtgg
    61921 acggtgaaat caaatatgtc gaaacggaca ataccggcga tgacaattac gacggcctgc
    61981 actaggttcg cgcctgcctg cgtgggcgtt ccatgcgtcg ccgtgtctac aatccggatc
    62041 gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga aggcaaattc gagcgcatta
    62101 gctgggaaga agcctacgac atcatcgcga ccaatatgca gcgcctgatc aaagagtacg
    62161 gcaacgagtc tatctatctg aactatggca ccggtacgct gggcggcacc atgacccgct
    62221 cctggccgcc gggaaatacc ctggtcgcgc ggctgatgaa ctgctgcggc ggctatctga
    62281 accattacgg cgactactcc tccgcgcaaa ttgcggaagg tttgaactat acctacggcg
    62341 gctgggcaga tggcaacagc ccgtcggata tcgaaaacag taagctggta gtgctgtttg
    62401 gtaataaccc tggcgaaacg cgaatgagtg gcagtggggt gacttactat cttgaacagg
    62461 cacgccagaa atctaatgcc cgcatgatca tcatcgatcc gcgctatacc gacaccggtg
    62521 ccgggcgcga agatgagtgg atccctattc gtccgggaac agatgccgca ctggttaacg
    62581 gtctggcgta cgtcatgatc actgaaaacc tggtggatca ggcattcctc gataaatatt
    62641 gcgttggcta cgatgagaaa accctgccag ccagtgcgcc gaaaaatggc cactataaag
    62701 cttatattct gggtgaaggg ccagatggcg tggctaaaac accggaatgg gcctcacaaa
    62761 tcaccggtgt tccggcagac aaaatcatca aactggctcg tgaaatcggc agtaccaaac
    62821 cggcgtttat cagccaggga tggggcccgc agcgtcacgc taacggtgaa atcgcaaccc
    62881 gtgctatctc gatgctggcg attctgaccg gtaacgttgg tattaacgga ggcaacagcg
    62941 gcgcgcgtga aggttcatac agcttaccgt ttgtccgtat gccgaccttg gaaaacccga
    63001 tccagaccag catttcgatg tttatgtgga ccgatgccat tgaacgtggc ccggaaatga
    63061 cggcgctgcg tgatggtgta cgcgggaaag ataagctgga tgtgccgatc aaaatgatct
    63121 ggaactatgc cggtaactgc ctgattaacc agcattctga aatcaaccgt acccatgaaa
    63181 tccttcagga tgataagaag tgcgagctga ttgtggttat cgactgccac atgacctcat
    63241 cggcgaaata tgctgacatc ctgctgcctg actgcaccgc ttccgaacag atggactttg
    63301 cgctggatgc atcctgcggg aatatgtctt acgtgatttt caacgatcag gtgattaaac
    63361 cgcgctttga atgtaagacc atctatgaaa tgaccagcga actggcaaaa cgtcttggcg
    63421 ttgagcaaca gtttactgaa ggccgtacac aggaagagtg gatgcggcat ctgtatgccc
    63481 agtcgcggga agcgattcct gaactaccaa cgtttgaaga gttccgcaag caggggatct
    63541 ttaaaaagcg cgacccacaa gggcaccacg ttgcttataa agctttccgt gaagatcctc
    63601 aggcaaattc actgaccacg ccatcgggca aaattgagat ttattcgcag gcgctggctg
    63661 acattgccgc tacctgggaa ttgccagaag gagatgtgat cgatccactg ccgatctaca
    63721 cgccgggctt tgaaagttat caggatccgt tgaacaaaca gtatccgctg cagcttacgg
    63781 gtttccacta taaatctcgc gttcactcaa cttacggcga cgttgatgtg ctgaaagcag
    63841 cttgccgtca ggaaatgtgg atcaacccgc ttgatgcgca aaaacgcggt atcaacaacg
    63901 gcgataaagt caggatcttt aacgatcttg gtgaggttca tattgaggcg aaagtgacgc
    63961 cacgaatgat gccgggtgtg gtcgcactgg gtgaaggtgc ctggtatgac ccggatacaa
    64021 aacgtgtcga taagggcggt tgtatcaacg tactgaccac tcaacgtccg tctcctctcg
    64081 ctaaggggaa tccgtcacat acaaaccttg ttcaggttga aaaggtgtaa ggagtaaccg
    64141 atgacaaccc agtatggatt ttttattgat tccagccgtt gcaccggttg caaaacctgc
    64201 gagctggcct gtaaagacta caaagatttg acgccagaag tcagcttccg ccgcatttat
    64261 gaatatgctg gcggcgactg gcaggaagat aacggtgtct ggcaccagaa cgtgtttgcc
    64321 tactatctgt cgatttcatg taaccactgc gaagatccgg cttgtactaa agtctg
//
LOCUS       DAJTOR010000005        54599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTOR010000005 DAJTOR010000000
VERSION     DAJTOR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.005"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            8..898
                     /gene="cyoE"
                     /locus_tag="OO249_000299"
     CDS             8..898
                     /gene="cyoE"
                     /locus_tag="OO249_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5634722.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            1047..2411
                     /locus_tag="OO249_000300"
     CDS             1047..2411
                     /locus_tag="OO249_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5634723.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(2539..3030)
                     /gene="yajQ"
                     /locus_tag="OO249_000301"
     CDS             complement(2539..3030)
                     /gene="yajQ"
                     /locus_tag="OO249_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5634724.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            3198..4109
                     /gene="panE"
                     /locus_tag="OO249_000302"
     CDS             3198..4109
                     /gene="panE"
                     /locus_tag="OO249_000302"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5634725.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSANLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            4072..4662
                     /gene="yajL"
                     /locus_tag="OO249_000303"
     CDS             4072..4662
                     /gene="yajL"
                     /locus_tag="OO249_000303"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5634726.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLQVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4716..6164)
                     /gene="thiI"
                     /locus_tag="OO249_000304"
     CDS             complement(4716..6164)
                     /gene="thiI"
                     /locus_tag="OO249_000304"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5634727.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIALQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            6370..6612
                     /gene="xseB"
                     /locus_tag="OO249_000305"
     CDS             6370..6612
                     /gene="xseB"
                     /locus_tag="OO249_000305"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5634728.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            6612..7511
                     /gene="ispA"
                     /locus_tag="OO249_000306"
     CDS             6612..7511
                     /gene="ispA"
                     /locus_tag="OO249_000306"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5634729.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSPDTSALEALADYIIQRNK"
     gene            7536..9398
                     /gene="dxs"
                     /locus_tag="OO249_000307"
     CDS             7536..9398
                     /gene="dxs"
                     /locus_tag="OO249_000307"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5634730.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            9453..10427
                     /gene="yajO"
                     /locus_tag="OO249_000308"
     CDS             9453..10427
                     /gene="yajO"
                     /locus_tag="OO249_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5634731.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(10481..10999)
                     /gene="pgpA"
                     /locus_tag="OO249_000309"
     CDS             complement(10481..10999)
                     /gene="pgpA"
                     /locus_tag="OO249_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5634732.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10977..11954)
                     /gene="thiL"
                     /locus_tag="OO249_000310"
     CDS             complement(10977..11954)
                     /gene="thiL"
                     /locus_tag="OO249_000310"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5634733.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGVRIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(12032..12451)
                     /gene="nusB"
                     /locus_tag="OO249_000311"
     CDS             complement(12032..12451)
                     /gene="nusB"
                     /locus_tag="OO249_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5634734.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(12471..12941)
                     /gene="ribE"
                     /locus_tag="OO249_000312"
     CDS             complement(12471..12941)
                     /gene="ribE"
                     /locus_tag="OO249_000312"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5634735.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(13030..14133)
                     /gene="ribD"
                     /locus_tag="OO249_000313"
     CDS             complement(13030..14133)
                     /gene="ribD"
                     /locus_tag="OO249_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5634736.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWGEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(14137..14586)
                     /gene="nrdR"
                     /locus_tag="OO249_000314"
     CDS             complement(14137..14586)
                     /gene="nrdR"
                     /locus_tag="OO249_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5634737.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14737..15276
                     /locus_tag="OO249_000315"
     CDS             14737..15276
                     /locus_tag="OO249_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5634738.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15575..16459
                     /gene="tsx"
                     /locus_tag="OO249_000316"
     CDS             15575..16459
                     /gene="tsx"
                     /locus_tag="OO249_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5634739.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTDLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16636..16983)
                     /locus_tag="OO249_000317"
     CDS             complement(16636..16983)
                     /locus_tag="OO249_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5634740.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(17112..18083)
                     /gene="secF"
                     /locus_tag="OO249_000318"
     CDS             complement(17112..18083)
                     /gene="secF"
                     /locus_tag="OO249_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5634741.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGEDLTQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(18094..19941)
                     /gene="secD"
                     /locus_tag="OO249_000319"
     CDS             complement(18094..19941)
                     /gene="secD"
                     /locus_tag="OO249_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5634742.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19969..20301)
                     /gene="yajC"
                     /locus_tag="OO249_000320"
     CDS             complement(19969..20301)
                     /gene="yajC"
                     /locus_tag="OO249_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5634743.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(20324..21451)
                     /gene="tgt"
                     /locus_tag="OO249_000321"
     CDS             complement(20324..21451)
                     /gene="tgt"
                     /locus_tag="OO249_000321"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5634744.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(21507..22577)
                     /gene="queA"
                     /locus_tag="OO249_000322"
     CDS             complement(21507..22577)
                     /gene="queA"
                     /locus_tag="OO249_000322"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5634745.1"
                     /translation="MRVTDFFFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTSVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22670..23251
                     /gene="acpH"
                     /locus_tag="OO249_000323"
     CDS             22670..23251
                     /gene="acpH"
                     /locus_tag="OO249_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR5634746.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALGTRFWQFYPRMMAQASHKAL"
     gene            complement(23256..24978)
                     /gene="malZ"
                     /locus_tag="OO249_000324"
                     /pseudo
     CDS             complement(23256..24978)
                     /gene="malZ"
                     /locus_tag="OO249_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(25134..26507)
                     /gene="proY"
                     /locus_tag="OO249_000325"
     CDS             complement(25134..26507)
                     /gene="proY"
                     /locus_tag="OO249_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5634747.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(26583..27902)
                     /gene="brnQ"
                     /locus_tag="OO249_000326"
     CDS             complement(26583..27902)
                     /gene="brnQ"
                     /locus_tag="OO249_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5634748.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(28309..29604)
                     /gene="phoR"
                     /locus_tag="OO249_000327"
     CDS             complement(28309..29604)
                     /gene="phoR"
                     /locus_tag="OO249_000327"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5634749.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(29662..30351)
                     /gene="phoB"
                     /locus_tag="OO249_000328"
     CDS             complement(29662..30351)
                     /gene="phoB"
                     /locus_tag="OO249_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5634750.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            30541..31743
                     /gene="sbcD"
                     /locus_tag="OO249_000329"
     CDS             30541..31743
                     /gene="sbcD"
                     /locus_tag="OO249_000329"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5634751.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALAESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31740..34883
                     /gene="sbcC"
                     /locus_tag="OO249_000330"
     CDS             31740..34883
                     /gene="sbcC"
                     /locus_tag="OO249_000330"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5634752.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQVSGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVKAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            35009..36241
                     /gene="araJ"
                     /locus_tag="OO249_000331"
     CDS             35009..36241
                     /gene="araJ"
                     /locus_tag="OO249_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5634753.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMFFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAGE
                     TTGVGYSLGVHYLLPDAA"
     gene            complement(36340..37250)
                     /gene="mak"
                     /locus_tag="OO249_000332"
                     /pseudo
     CDS             complement(36340..37250)
                     /gene="mak"
                     /locus_tag="OO249_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
     gene            37375..38286
                     /gene="rdgC"
                     /locus_tag="OO249_000333"
     CDS             37375..38286
                     /gene="rdgC"
                     /locus_tag="OO249_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5634754.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(38364..38447)
                     /locus_tag="OO249_000334"
     CDS             complement(38364..38447)
                     /locus_tag="OO249_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5634755.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(38444..38785)
                     /locus_tag="OO249_000335"
                     /pseudo
     CDS             complement(38444..38785)
                     /locus_tag="OO249_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38933..39217)
                     /gene="ppnP"
                     /locus_tag="OO249_000336"
     CDS             complement(38933..39217)
                     /gene="ppnP"
                     /locus_tag="OO249_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5634756.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(39289..39966)
                     /locus_tag="OO249_000337"
     CDS             complement(39289..39966)
                     /locus_tag="OO249_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5634757.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(40224..40415)
                     /gene="yaiA"
                     /locus_tag="OO249_000338"
     CDS             complement(40224..40415)
                     /gene="yaiA"
                     /locus_tag="OO249_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5634758.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(40465..40989)
                     /gene="aroL"
                     /locus_tag="OO249_000339"
     CDS             complement(40465..40989)
                     /gene="aroL"
                     /locus_tag="OO249_000339"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5634759.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(41172..41630)
                     /locus_tag="OO249_000340"
     CDS             complement(41172..41630)
                     /locus_tag="OO249_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5634760.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            41750..42559
                     /gene="proC"
                     /locus_tag="OO249_000341"
     CDS             41750..42559
                     /gene="proC"
                     /locus_tag="OO249_000341"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5634761.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(42576..43691)
                     /gene="adrA"
                     /locus_tag="OO249_000342"
                     /gene_synonym="dgcC"
     CDS             complement(42576..43691)
                     /gene="adrA"
                     /locus_tag="OO249_000342"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5634762.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(43793..44113)
                     /gene="psiF"
                     /locus_tag="OO249_000343"
     CDS             complement(43793..44113)
                     /gene="psiF"
                     /locus_tag="OO249_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5634763.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(44232..45647)
                     /gene="phoA"
                     /locus_tag="OO249_000344"
     CDS             complement(44232..45647)
                     /gene="phoA"
                     /locus_tag="OO249_000344"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5634764.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(45748..46008)
                     /gene="iraP"
                     /locus_tag="OO249_000345"
     CDS             complement(45748..46008)
                     /gene="iraP"
                     /locus_tag="OO249_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5634765.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            complement(46235..46392)
                     /locus_tag="OO249_000346"
                     /pseudo
     CDS             complement(46235..46392)
                     /locus_tag="OO249_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            46470..47564
                     /gene="ddlA"
                     /locus_tag="OO249_000347"
     CDS             46470..47564
                     /gene="ddlA"
                     /locus_tag="OO249_000347"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5634766.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLRLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(47588..47800)
                     /locus_tag="OO249_000348"
     CDS             complement(47588..47800)
                     /locus_tag="OO249_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5634767.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGAVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            48060..48368
                     /locus_tag="OO249_000349"
     CDS             48060..48368
                     /locus_tag="OO249_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5634768.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(48427..49521)
                     /gene="yaiW"
                     /locus_tag="OO249_000350"
     CDS             complement(48427..49521)
                     /gene="yaiW"
                     /locus_tag="OO249_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5634769.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(49534..50754)
                     /gene="sbmA"
                     /locus_tag="OO249_000351"
     CDS             complement(49534..50754)
                     /gene="sbmA"
                     /locus_tag="OO249_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5634770.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            51106..52263
                     /gene="ampH"
                     /locus_tag="OO249_000352"
     CDS             51106..52263
                     /gene="ampH"
                     /locus_tag="OO249_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5634771.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(52264..52887)
                     /gene="iprA"
                     /locus_tag="OO249_000353"
     CDS             complement(52264..52887)
                     /gene="iprA"
                     /locus_tag="OO249_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR5634772.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FFILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(52975..>54599)
                     /locus_tag="OO249_000354"
     CDS             complement(52975..>54599)
                     /locus_tag="OO249_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5634773.1"
                     /translation="NNGSQVNVTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDSY
                     STVNLTESTGWNNYSNLYTNTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHN
                     GNVISGVFDINSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNG
                     TELDNSSVDNVVAATGNYKVRIDNATGAGTIADYKDKELIRVNDANTNATFSAANKAD
                     LGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGL
                     ADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGD
                     MNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNS
                     DAWGFGLKAGYDFKLGDAGYETPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELG
                     VDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSF
                     TKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT        13505 a        14479 c        13642 g        12973 t
ORIGIN      
        1 ggcggttatg atgtttaagc aatacctgca agtaacgaaa ccaggcatca tctttggcaa
       61 cctgatctcg gtgattgggg gattcctgct ggcctcaaag ggcagcattg attatcccct
      121 gtttatctac acgctggttg gggtgtcact ggttgtggcg tcgggttgtg tgtttaacaa
      181 ctacatcgac agggatatcg acagaaagat ggaaaggacg aagaatcggg tgctggttaa
      241 aggcctgatc tctcctgctg tctcgctggt gtacgccacc ttgctgggta ttgctggctt
      301 tatgctgctg tggtttggcg cgaatccgct ggcctgctgg ctgggggtga tgggctttgt
      361 ggtttatgtc ggcgtctata gcctgtacat gaaacgccac tcagtctacg gcacattgat
      421 tggttcgctc tccggcgctg cgccgccggt gattggctac tgtgcagtaa ccggcgagtt
      481 cgatagcggc gcagcgatcc tgctggctat cttcagcctg tggcagatgc ctcactccta
      541 tgccatcgcc attttccgct ttaaggatta ccaggcggca aacattccgg tattgccagt
      601 ggtaaaaggc atttcggtgg cgaagaatca catcacgctg tatatcatcg cctttgccgt
      661 tgccacgctg atgctctctc ttggcggtta cgctggatat aaatatctgg tggtcgccgc
      721 ggcggttagc gtctggtggt taggtatggc tctgcgcggt tataaagttg ctgatgacag
      781 aatctgggcg cgcaagctgt tcggcttctc tatcatcgcc atcactgccc tctcggtgat
      841 gatgtccgtt gattttatgg taccggactc gcatacgctg ctggctgctg tgtggtaaca
      901 aaacctctct attaaaaagg tgctacggca ccttttttct tagcattaga aacatatccc
      961 tctcgaaata tttactaaaa aatccgcatg tttaccccat tcgtttgccg ctttacacta
     1021 gtcgcgaatt taaaacagag gtggtaatga acgattataa aatgacgcca ggtgagaggc
     1081 gcgcgacctg gggtttaggg accgtattct cgttgcgcat gctgggcatg tttatggttc
     1141 tgccggttct gaccacgtat ggcatggctc tgcaaggtgc cagcgaagca ttaatcggta
     1201 ttgccattgg tatttatggt ctgactcagg ccgtttttca gattccgttt ggcctgcttt
     1261 ccgaccgtat tggtcgcaaa ccattaattg tcggtgggct ggcggtgttt gccgccggta
     1321 gcgttatcgc tgcgctctct gactccatct ggggaattat tctaggccgg gcgctacaag
     1381 gctccggtgc gattgccgct gccgttatgg cgctgctttc cgatctcacg cgcgaacaaa
     1441 accgcaccaa agcaatggcg tttatcggcg tgagctttgg cattaccttt gccattgcga
     1501 tggtgcttgg cccgatcatc actcacaaac ttgggctgca cgcgctgttc tggatgatcg
     1561 ctattctggc aacgaccggc attgcgttga ccatttgggt tgtgcccaac agtagcactc
     1621 acgtacttaa tcgtgagtcc ggaatggtga aaggcagttt cagtaaagtg ctggcggaac
     1681 cgcggctgct gaaactcaac tttggcatta tgtgtctgca tattttgctg atgtcgacgt
     1741 ttgttgccct gcccggacaa ctggctgatg cagggttccc ggcggctgaa cactggaagg
     1801 tctatctggc gacaatgcta atcgcctttg gctcggtcgt gcctttcatt atctacgctg
     1861 aagttaagcg caaaatgaag caagtctttg tcttctgcgt cggattgatc gtggttgcgg
     1921 aaattgtgtt gtggaacgcg caaacgcagt tctggcaact ggtggtcggc gtgcagcttt
     1981 tctttgtagc gtttaatttg atggaagccc ttctgccttc acttatcagt aaagagtcgc
     2041 cagcaggtta caaaggtaca gcgatgggtg tttactccac cagccagttt cttggcgtgg
     2101 cgattggcgg ttcactgggc ggctggattg acggcatgtt tgacggtcag ggcgtatttc
     2161 tcgctggcgc aatgctggcc gcagtgtggc tggcagtcgc cagtaccatg aaagaaccgg
     2221 cgtatgtcag cagtttgcgc attgaaatcc cggcgaacat tgccgcaaac gaggcgttaa
     2281 aagtgcgttt gctagaaact gaaggcatca aagaagtgtt gattgcagaa gaagaacatt
     2341 cagcttatgt gaaaatcgac agcaaagtga cgaatcgctt tgatgttgaa caggcaattc
     2401 gccaggcata aaagcgaggc gaaattgcct gctgcgctac gctcatcagg cctactaagc
     2461 tcctgattta caagcctttg taggccgaac caggcgttca tgccgcatct gacatgaaca
     2521 aagcgcaggt tgtcgcgatt aatcgcggaa gtttttgaac tggaacggct gacccagatc
     2581 gccaccgcgc accatcgcca tgacagcctg caaatcatca cgagatttgc cagtcacgcg
     2641 gatctcatcg ccctgaattt gcgcctgcac tttcagtttg ctgtctttga tcatcttgac
     2701 gatttttttc tgggtcgcgc tctcaatgcc ctgcttcagt ttcgcttcca caaaccaggt
     2761 tttaccgcta tgaacgatat tttccggtac atccagtgaa ctgccttcaa tgccgcgctt
     2821 cagcagcttg gcacgcagaa tatccagcaa ctgattgacc tggaaatcgg actcgctcaa
     2881 cactttgatg gttttgctgg cgtcgttcag ctcaaatgag gcttcaacgt tacggaagtc
     2941 aaaacgggac tccacttcgc ggctcgcgtt atcgaccgcg ttacgtgctt cctgaagatc
     3001 aacttcagag acaatatcga aagatggcat cttttcctct cccttcattt ttgatgcgaa
     3061 gcataatacc cgcaaagtta aataaccgac agtcctgcac caggacgtat cccttaattg
     3121 tgcatagtca caacagcatg cacaattaag ggatagcctt attgaacacc cggagtggtt
     3181 gcgggtgagg aggaacaatg aaaattaccg tattgggatg cggtgcctta gggcaattat
     3241 ggcttacagc actttgcaaa cagggtcatg aagttcaggg ctggctgcgc gtaccgcaac
     3301 cttattgtag tgcgaatctg gttgagacag atggttcgat atttaacgaa tcgctgaccg
     3361 ccaacgatcc cgattttctc gccaccagcg atctgctcct ggtgacgctg aaagcatggc
     3421 aggtttccga tgccgtcaaa agcctcgcgt ccacactgcc tgtaaccacg ccaatactgt
     3481 taattcacaa cggcatgggc accatcgaag agttgcaaaa tattcagcag ccattactga
     3541 tgggcaccac cacccatgcc gcccgccgcg acggcaatgt cattattcat gtggcaaacg
     3601 gtatcacgca tattggtccg gcacggcaac aggatggcga ttacagttat ctggcggata
     3661 ttttacaaac cgtgttgcct gacgttgcct ggcataacaa tattcgcgcc gagctgtggc
     3721 gcaagctggc agtcaactgc gtgattaatc cactgaccgc catctggaat tgcccgaacg
     3781 gtgaattacg tcatcatccg caagaaatta tgcaaatatg cgaagaagtc gcggcagtga
     3841 tcgaacgcga agggcatcat acttcagcgg aagatttgcg tgattacgtg atgcaggtga
     3901 ttgatgccac agcggaaaat atctcgtcga tgttgcagga tatccgcgcg ctgcgccaca
     3961 ccgaaatcga ctatatcaat ggttttctct tacgccgcgc ccgcgcgcat gggattgccg
     4021 taccggaaaa cacccgcctg tttgaaatgg taaaaagaaa ggagagtgaa tatgagcgca
     4081 tcggcactgg tttgcctcgc ccctggtagt gaagagactg aagccgtcac cacgatcgac
     4141 ctcttggttc gcggcggtat caaagtcacc actgccagcg tagccagcga tggcaacctg
     4201 gcaattacct gctcgcgcgg cgtgaagctg ctggcggatg cgccgctggt cgaggtggct
     4261 gatggcgaat atgacgtgat cgtactgcct ggtggcatta aaggcgcaga gtgttttcgc
     4321 gatagcactc tgctggttga aaccgttaaa cagttccacc gttccgggcg tatcgtcgcg
     4381 gctatttgcg ccgcgccagc caccgtgctg gtgccgcacg atatcttccc gattggtaat
     4441 atgaccggct tcccgacgct gaaagacaaa attcccgctg aacaatggca ggacaagcgc
     4501 gtcgtctggg atgcacgggt aaaattgctg accagccagg ggccggggac agctatcgac
     4561 tttggtctga aaattatcga cctgcaggtt gggcgcgaga aagcccatga agtggcatca
     4621 caactggtga tggcggcagg gatttataat tattacgagt agtgtcggat gcggtaagcg
     4681 tcgcatctga ccagatgcga cgttacaacc acaaattacg ggcgatatac cttcacattg
     4741 ttaaagccct gctcgcgcag atagagcgcc tgcagacggc tcatcacccc gcgctcacac
     4801 cacagtagcc aggttctgtt ctggtcgaga tcgccaaact tggtgctcag tttatagaac
     4861 ggcagagaaa ctacatcaat cccttcgact ttcagtggct tatcttcctg ttcatcgata
     4921 gaacggatat cgaggatcac gtcgttcggg ccgaagccat tgacggtttc cacttccacc
     4981 acttcctgct cggtctgcag ggcgatttcg cggatatcaa cgttattcgc ttcttcaacc
     5041 actttatcga gaatgctgaa gtcgaatttc tcttcttccg cttcaatctt cgacttaacc
     5101 gctttcaccg tcgggctttt ggagataaca ccgcaatatt ccggcatcgt gcgagcaaag
     5161 tcttcggtgc caatctggcg agccaggttg atgatgtgct ctttgtcgta agaaatcagc
     5221 ggacgcagga tcagcgtatc ggagacgtta tcaatcaggc gcaggttggt cagcgtctga
     5281 ctggacacct ggccgagcgc ttcgccggtg accagcgcct gtacgccgta acgttcagcc
     5341 actttagacg cggcacgcac catcatacgt ttgaggataa cgcccatctg accgtcgtcg
     5401 attttctcga gaatttcccc gacgaccggt tcgaaattaa tagcgacaaa acgcacgcgg
     5461 tgggagctgc caaaacggtt ccacagataa tgcgccacct gacgcacgcc aatttcatgc
     5521 gccgcgccgc caaggttaaa gaaacagtaa tgcacgcggc agccgcgacg catcaacata
     5581 taactggaaa caccggagtc gaaaccaccg gaaatgagcg acagcacatc ttcctgggtg
     5641 ccgatcggga aaccgccaat accttcgtag cggcctttaa tcagcagaag acgatcgtct
     5701 tccacttcca gatggacagt cacttccgga ttggtcagct tcacgcgcgc ggactcaata
     5761 tgctgattta aaccgccgcc gacgtaacgt tccacatcaa tcgagctaaa atcatgtttg
     5821 ccacggcgct tcacgcgtac gcagaaggtt ttgccttcca gctgatcgcg atactgaacc
     5881 aacgctttct caaaaatatc gtgcatgtcg gtaaacggca cgtcttcgac ttcgagaata
     5941 tggtggatac ccggaatacg ggtcagggcg tcgcgaatag ccagacgctg gttttcatct
     6001 tttgcacgaa cttcgatgtt atcccagtgg cggacgacag cgagcgtctc atcatagtgc
     6061 tttaaaacgt tacgaatgtt cccggtaagg atttttataa agcgcaagcg cacagattgg
     6121 cttttgatgg tgatttccgg gaacaattta atgataaact tcatggcggc aatggttcgt
     6181 tggcaagcct taagcgactt gtatagggaa aaatacagca gcccacacct gcggctgcat
     6241 ccaggcgcgg aagtatacca ctaacatcgc tttgctgcgc acatcacctt accattgcgc
     6301 gttatttgct atttgccctg agtccgttac catgacgggg caaaaaatat tgagagtcag
     6361 acattcatta tgccgaagaa aaatgaggct cccgccagct ttgaaaaggc gctgagcgag
     6421 ctggaacaga ttgtaacccg tctggaaagt ggcgacctgc cgctggaaga ggcgctgaac
     6481 gagttcgaac gcggcgtgca gctggcacgt caggggcagg ccaaattaca acaagccgaa
     6541 cagcgcgtac aaattctgct gtctgacaat gaagacgcct ctctaacccc ttttacaccg
     6601 gacaatgagt aatggacttt ccgcagcaac tcgaagcctg cgttaagcag gccaaccagg
     6661 cgctgagccg ttttatcgcc ccactgccct ttcagaacac tcccgtggtc gaaaccatgc
     6721 agtatggtgc attattaggt ggtaagcgtc tgcgaccctt cctggtttat gccaccggtc
     6781 atatgtttgg cgttagcaca aacacgctgg acgcacccgc tgccgccgta gagtgtatcc
     6841 acgcttactc attaattcat gatgatttac cggcgatgga tgatgacgat ctgcgtcgcg
     6901 gtttgccgac ctgccatgtg aagtttggcg aagcaaacgc gattctcgct ggcgacgctt
     6961 tacaaacgct ggcgttctcg attttaagcg atgctgatat gccggaagtg tcggatcgcg
     7021 acagaatttc gatgatttct gaactggcga gcgccagcgg tattgccgga atgtgcggtg
     7081 gtcaggcatt agatttagac gcggaaggca aacacgtacc tctggacgcg cttgagcgta
     7141 ttcatcgtca taaaaccggc gcattgattc gcgccgccgt tcgccttggt gcattaagcg
     7201 ccggagataa agggcgtcgt gctctgccag tactcgacaa gtacgcagag agcatcggcc
     7261 ttgccttcca ggttcaggat gacatcctgg atgtggtagg agatactgca acgttgggaa
     7321 aacgccaggg tgccgaccag caacttggta aaagtaccta ccctgcactt ctgggtcttg
     7381 agcaagcccg gaagaaagcc cgggatctga tcgacgatgc ccgtcagtcg ctgaaacaac
     7441 tggctgaaca gtcacccgat acctcggcac tggaagcgct agcggactac atcatccagc
     7501 gtaataaata aacaataagt attaataggc ccctgatgag ttttgatatt gccaaatacc
     7561 cgaccctggc actggtcgac tccacccagg agttacgact gttgccgaaa gagagtttac
     7621 cgaaactctg cgacgaactg cgccgctatt tactcgacag cgtgagccgt tccagcgggc
     7681 acttcgcctc cgggctgggc acggtcgaac tgaccgtggc gctgcactat gtctacaaca
     7741 ccccgtttga ccaattgatt tgggatgtgg ggcatcaggc ttatccgcat aaaattttga
     7801 ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg cggcctgcac ccgttcccgt
     7861 ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca ttcatcaacc tccatcagtg
     7921 ccggaattgg tattgcggtt gctgccgaga aagaaggcaa aaatcgccgt accgtctgtg
     7981 taatcggcga tggcgcgatt accgcaggca tggcgtttga agcgatgaat cacgcgggcg
     8041 atatccgtcc tgatatgctg gtggtcctca acgacaacga aatgtcgatt tccgaaaatg
     8101 tcggcgcgct caacaaccat ctggcgcagc tgctttccgg taagctttac tcttcactgc
     8161 gcgaaggcgg gaaaaaagtt ttctctggcg tgccacccat taaagagctg ctcaaacgta
     8221 ccgaagaaca tattaaaggc atggtagtcc ctggcacgtt gtttgaagag ctgggcttta
     8281 actacatcgg cccggttgac ggtcacgatg tgctggggct tatcaccacg ctgaagaaca
     8341 tgcgtgacct gaaaggcccg cagttcctgc atatcatgac caaaaaaggt cgtggttatg
     8401 aaccggcaga aaaagaccca atcactttcc acgccgtgcc taaatttgat ccctccagcg
     8461 gttgtttgcc gaaaagtagc ggcggtttac cgagctattc aaaaatcttt ggcgactggt
     8521 tgtgcgaaac ggcggcgaaa gataacaagc tgatggcgat tactccggcg atgcgtgaag
     8581 gttccggcat ggtcgagttt tctcgtaaat tcccggatcg ctacttcgac gtggcaattg
     8641 ccgagcaaca cgcagtgacc tttgccgccg gtctggcgat tggtgggtac aaacccattg
     8701 tcgcgatcta ctccactttc ctgcaacgcg cctatgatca ggtgctgcat gacgtggcaa
     8761 ttcaaaagct tccggtcctg ttcgccatcg accgcgcggg cattgttggt gctgacggtc
     8821 aaacccatca gggtgctttt gatctctctt acctgcgctg cataccggaa atggtcatta
     8881 tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta taccggctat cactataacg
     8941 atggcccgtc agcggtgcgc tacccgcgtg gcaacgcggt cggcgtggaa ctgacgccgc
     9001 tggaaaaact accaattggc aaaggcattg tgaagcgtcg tggcgagaaa ctggcgatcc
     9061 ttaactttgg tacgctgatg ccagaagcgg cgaaagtcgc cgaatcgctg aacgccacgc
     9121 tggtcgatat gcgttttgtg aaaccgcttg atgaaacgtt aattctggaa atggccgcca
     9181 gtcatgaagc actggttacc gtagaagaaa acgccattat gggcggtgca ggcagcggag
     9241 tgaacgaagt gctaatggcc catcgtaaac cagtccccgt gctgaacatt ggcctgcctg
     9301 acttctttat tccgcaagga acgcaggaag aaatgcgcgc cgaactcggc ctcgatgccg
     9361 ccggtatgga agccaaaatc aaggcctggc tggcataatc cctactccac tcctgctatg
     9421 cttaagaaat tattcatggc aggagtggca gcatgcaata caacccctta ggaaaaaccg
     9481 accttcgcgt ttcccgactt tgcctcggct gtatgacctt tggcgagcca gatcgtggta
     9541 atcacgcatg gacactgccg gaagagagca gccgtcccat aattaaacgc gcactggaag
     9601 gcggcataaa tttctttgat accgccaata gctattccga tggtagcagc gaagagatcg
     9661 ttggtcgcgc actgcgggat ttcgcccgtc gtgaagacgt ggtcgttgcc accaaagtgt
     9721 tccatcgcgt tggtgattta ccggaaggat tatcccgtgc acaaattttg cgctctatcg
     9781 acgacagcct gcgacgtctc ggcatggatt atgtcgatat cctgcaaatt catcgctggg
     9841 attacaacac gccgatcgaa gagacgctgg aagccctgaa cgacgtggta aaagccggga
     9901 aagcgcgtta tatcggcgcg tcatcaatgc acgcttcgca gtttgctcag gcactggaac
     9961 tccaaaaaca gcacggctgg gcgcagtttg tcagtatgca ggatcactac aatctgattt
    10021 atcgcgaaga agagcgcgag atgctgccac tgtgttatca ggagggcgtg gcggtgattc
    10081 cgtggagccc gctggcgcgg gggcgactga cgcgtccgtg gggagaaact accgcacgac
    10141 tggtgtctga tgaggtgggg aaaaatctct ataaagaaag cgatgaaaat gacgcgcaga
    10201 tcgcagagcg gttaacgggc gtcagtgaag aactcggtgc aacacgagca caagttgcgc
    10261 tggcttggtt gttgagtaaa ccgggcattg ccgcaccgat tatcggtaca tcgcgggaag
    10321 aacagcttga tgagctattg aacgcggtgg atatcacttt gaagccggaa cagattgccg
    10381 aactggaaac gccgtataaa ccgcatccgg tagtaggatt taaataagag tccaggcctg
    10441 atgagacgca acaagcgtca catcaggcat cggtgcacaa ctacgacaga atgcccagcg
    10501 gccagtgatg accaataaag tacaggatgc cagcggagat caccccggcg acaatatcat
    10561 ctatcatgat ccccatgccg ccatgcacat tgcgatcaaa ccagcggatc ggccacggtt
    10621 tccacatatc cagaatacgg aaaatcacaa acccggcggc aacccactgc cagtcattgg
    10681 tcggcagcgc catgagcgtg atccacatac caataaattc gtcccagaca atgctgccgt
    10741 gatcgtgcac gcccatatct tttgcggtct gatgacaaag atagacgccg atacagatcc
    10801 ccagcatcac caccagcgag tagagctgcc agggcaaaaa ggtcatcaga taccagaacg
    10861 gaatcgctgc cagcgagccc atcgtcccag gaacgatcgg gcttaatcca cttccgaatc
    10921 cgacagcaag tagatgccac ggattactca tcttcaggcg acttttcgcg acatctttat
    10981 ggcgtggcaa aatggtcata tcctttccag tctaacgtaa caggttcacc gtcacgaata
    11041 aaacaaagcc cttcgatatc ggcggtcatt tgcccgatac aggtaaacgg tacgcccagg
    11101 tgtccgagag ccacatccag cgctccacgg ttcagttccg gcaccgtgaa acacaactcg
    11161 taatcttcac cgccagagag cgcccagcgc agcgcctgtt ccggttcaac atggcgagaa
    11221 agcgcatcag aaaacggcag caatgccagg tcaatacgta cgccgcagtc gctggctttc
    11281 acgatatgcc cgagatcgga aatcagaccg tcagagagat cgatagctga atttgccaga
    11341 tcgcgcagtg cctgcccctg taaaatacgc ggcgatggac ggagatgacg tttgatcaag
    11401 tagtccgcat ctttagcatc ggcaacctgc aaacggtttt gcaaaatcgc cagcccggcg
    11461 gcgctatcgc ccggtgtacc ggtcacatag atccagtcac ccggtttcgc cccagagcgc
    11521 gttaaggctc gtcccatcgg aacaaagccg tggataccca acgtcattga taatggccca
    11581 cgcgtggtat cgccgccaat gagttgcata tcgtaataat tgagaagatc aaacaaactg
    11641 tcgctgaagg actcaagcca cgcttcgtct acgtccggca aggttaatgc cagcgtcagc
    11701 caggccggat cggcccccat cgctgccaga tcgcttaggt tcaccgccag tgctttatac
    11761 gccagatcag caggatcgat atcagggagg aagtggttgc ccgccaccag cgtatcagtg
    11821 ctgatcgcca gggtctgttt ctcggggata ttgagaagtg cgcaatcgtc gccgatgccc
    11881 agttcgacat caagacgaga acttcttaca cggtcaaaat aacgggcaat cagggagaac
    11941 tcgccacatg ccatacgtta tgcctcagca ggtaaaaaag aaaaggccgg cgatcatgga
    12001 acggtcttcc gtgaatctac cggcctggat attacttttt gttagggcga atcacaggtg
    12061 ctgctttatc gagtacaccg ttgacgaact tatggctgtc ttctgcgccg aacgatttcg
    12121 ccagttcgat cgcttcgtta atggccactt tgtatggcac atcgctacgt ttagacagtt
    12181 cgtacagcgc aatgcgcagt actgcttttt ctacctgtcc cagttcttcc agcagacggg
    12241 acaggtatgg cttcatcagt ccgtcgaggt atgcggtatt agtcgccacc ccggccagca
    12301 gctcacggaa gtacaggacg tcaacgtctt ttacatcctg ttcagccagg aactggtatt
    12361 caacatcagc gatgtcgttc tgggacaact gccaggagta gagcgcctgg acggcacact
    12421 cacgagcgcg gcgacgagca gcaggtttca cggatttccc cttactaatt tcaggccttg
    12481 atggctttca atacattaat catttcaagc gcggtcagtg cagcttctgc acctttgttg
    12541 ccagctttgg tgccagcacg ttcgatcgct tgttcaatgc tttcagtggt cagaacccca
    12601 aaagcaaccg gaatttcgct gtcctgggca acatgcgcca ggccgttgct tgcaccacca
    12661 gcgacatatt caaaatgggc agtgccacca cgaataaccg tacccagcgc aatcaccgcg
    12721 tcgtatttac cggttttagc cagtgcaccc gccgccagcg gcagctcata ggcaccaggc
    12781 acccaaacaa cggtaatgtt ttcatctttt acctgaccga tacgtttcag tgcgtcaatt
    12841 gcaccttcca gcaggctgtc attgataaag ttgttgaaac gcgcgatggt gatggcgacg
    12901 cgagcgtccg gggtagcaac gttagcttca ataatgttca ttttcttcct tcgggttcga
    12961 gtatggcccc gcaggggggc ggattttagc ataatatttc gtgcgctgct tccctttcga
    13021 gccgggagat catgcaccca ctaaatgcag gcaaacatcc gggcctacat gacgtatctc
    13081 tttgaattta aattgggggg cgtcggctaa tttctcaagc cctggcaggg tgcataatcc
    13141 acgggcgtcg ctgcctaata gtttaggtgc gatatagaca atcagctcat cgactaaacc
    13201 cgcctgcagc aatgcgccag cgagcgttgg ccccgcttcc ccccagatgc tgttaatctg
    13261 ctgtttaccc agttgcatca tcagtacaac cagatccaga tgacctttat gctctggaat
    13321 cagcaaggta cgcaccgttt ccggccactc acgagaatct tcctgcgtac gcgcgaacca
    13381 ggtttcgccg ggctgctgca caatgtgatg ttccggcgtc acgcgatttt ggctatcaat
    13441 cacaatacgt accggctggc ggagattttg ttgcgggtag agcgcctgag tttgttcatc
    13501 cagttcggac caacgcaccg ttaaggcagg atcatccgcc agcaccgtgg cgctgctggt
    13561 taaaatggca tgactttgcg cgcgcagtcg ttgtacatca cgccgcgcct ggggcgaagt
    13621 gatccactgg ctttcgccgc tcgccatcgc cgtgcgacca tcaagcgatg cgccaagttt
    13681 taactgaata taaggaaagc cggtgcgcat ccgcttgaga aagcctttat tcaattgctc
    13741 ggcttcactc atcatcaggc cgtggctgac gtcaatgcca gcctgttgca gacggtaaag
    13801 tccacgccca gcgacctgcg ggttaggatc ttgcatcgag gcaaccacgc gcgccacacc
    13861 tgcggcaatt aaagcatcac agcaaggcgg cgtacggccg tgatggctgc aaggttcgag
    13921 tgtcacatac gccgttgcac ctttggcttt ttcacccgcc atacgcaacg cgtgtacttc
    13981 ggcatgtggt tcacccgctc gttgatggta accttcaccg acaatttcgc catctttgac
    14041 aatgacgcac ccgacatttg ggttggggtg ggtggtaaaa cgtcctcgtt gcgccagctt
    14101 tagcgcccgc gccatgtaat actcgtcctg cacggcttag tcctccaggc gcgcgatctc
    14161 ttcgccaaat tctttgatat cttcgaaact gcggtagaca gaggcaaaac ggatataggc
    14221 gactttatcg agctttttca attgctccat caccagattg ccaatcatct tgctcggtac
    14281 ttcgcgctca ccggtggcgc gcagctgcga tttaatatga ttgattgcca tttcaacgtc
    14341 atcggaactc accggacgtt tttccagcgc ccgcagcatt ccgctacgca atttctcttc
    14401 attaaacggt tcacgcacgt cgttgctttt tacaacacgc ggcataacca gctccgccac
    14461 ttcaaaggtg gtgaaacgtt cattacacac cagacactgc cgacggcggc gtacggatga
    14521 accctcgccc acgagacgag agtcaattac cttagtgtcc acggcgaaac agaatgggca
    14581 atgcatacgg cgtcctgacc aagtggttaa cggaaatcta ttttaccctg aactgacgat
    14641 acaacaaagg cgcaacgctt ttgagataaa ggatcgatgc tttcgctgct tttaccctct
    14701 accattaggc taattgcgac gagaaaggga cgacctatga atactaacgt ttttcgactg
    14761 ctcctgctgg gaagcctgtt cagccttagc gcctgtgtgc agcaaagtga agtgcgacag
    14821 atgaaacaca gcgtcagcac gctgaaccag gagatgacgc aactcaatca agaaacagtc
    14881 aaaatcactc agcaaaacag gctgaatgca aaatccagca gtgggggtta ccttctgcct
    14941 ggagcgaaaa caccggcaag actggaaagc cagatcggta ctttacgtat gtcgctggtg
    15001 aatattacgc ctgatacaga tggcaccacc cttacactac gtattcaggg tgagtcgaat
    15061 gatcccttgc ccgcattcag cggaactgtt gaatatgggc aaattcaggg aacaatagac
    15121 aactttcagg aaatcaatgt gcagaatcaa ttgattaatg cccctgccag cgtcctcgcc
    15181 cccagcgatg ttgatattcc gttacaatta aaggggattt ctgtggatca gttaggcttt
    15241 gtacgtatcc acgacattca acctgttatg cagtaaacgt atttcgggac gattttgtgc
    15301 gtcccgcaac atctttcccc gtcattttgt tactctgctt acatcacctg gattgatagt
    15361 aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt gtaaacgtga
    15421 acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg tgagcaatga
    15481 tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc gcctttttca
    15541 ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac tggcagccgg
    15601 tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg acaaaccgca
    15661 gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc acacccgttt
    15721 cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta aaaaagactg
    15781 gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact ccgatgctaa
    15841 aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt tctccatcga
    15901 caagctgacc aatactgacc ttaacttcgg tccgttcaaa gagtggtact tcgcgaacaa
    15961 ctacatttac gacatgggtc gtaataaaaa tggtcgccag agcacctggt acatgggtct
    16021 gggtaccgac atcgatactg atctgccgat gagcctgtcc atgaacgtct atgcgaaata
    16081 ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc gtttcaaaat
    16141 taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca tcggcttcac
    16201 caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca acggtattaa
    16261 gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact acgatcactg
    16321 gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg acgatgcaga
    16381 actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg gttacctggt
    16441 agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat ttctgattgt
    16501 taacaacgtg agctttgttc atgccggatg cggcgtaaac gctttgtccg gcccacaaaa
    16561 gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag cgcatcaggc
    16621 aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag cgaatgggtt
    16681 gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg tcgtaccata
    16741 ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac aaagcaattc
    16801 ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt gaacggtaag
    16861 ttcacgcagg ttggaataaa caaactcgcg ggaacagcga ccgcagaccc acggatatat
    16921 ttttaatgct ttttcgcgat agccgctttc taaccgcgca tagttttttg ggatgatagc
    16981 catgaagagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga tgcggggatt
    17041 ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt tcaacatcaa
    17101 cgggaacttg attacggcag aattgacggc tgatccgccc cttctttttc cactttctgc
    17161 tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc cacatagatg
    17221 gaagatgcag taccgatgga aacaccgata agcatggtca gcgagaagcc ttccagtacc
    17281 ggaccaccga agaggtacag catcaggata accatcaagg tagtaccgga tgtgatcaag
    17341 gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt accgcgacgg
    17401 atcttgcgga agttttcacg aatacggtcc gataccacga tactgtcgtt aagcgagtaa
    17461 ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg gaataacgac
    17521 aaaataccca gcgtaatgat aacgtcgtgc gccagcgcaa taacaacccc tgccgccagt
    17581 cgccactcaa agcgaaaacc tacgtacaca aggatagaca gcagcgccgc catcaacgcc
    17641 atcgcaccgg tttgcgtaag gtcttccccc acgctcggac cgacgaactc aatacgcttc
    17701 actgctgcat tctgattggt ggattcgtta atcaccttca gaacctggct gcccagcacc
    17761 tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg gctgctacca
    17821 aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg cattacgtca
    17881 atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa atccagcccc
    17941 cagttaaagc cgcgcacgcc cataataacg atagcggcga ttaacagcag accagagatg
    18001 ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg gtttagttgt
    18061 tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct tgacgcgctt
    18121 gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa acatcgacgt
    18181 cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac ccactgcgta
    18241 caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac tgaatgcgcc
    18301 acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact cttctttaat
    18361 acgttcgttg atcagtacgt tcgcatcgac cgccaccgca agggttaaga cgatacccgc
    18421 gatacctggc atactcagcg ttgcgcctgg caacagcgac ataatgccga cgattaagat
    18481 caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga agatgatcat
    18541 gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgtt caatgttctg
    18601 catacccagg gttgggccaa tggtacgttc ttcaacaatc tgaatcggcg cgatcaacgc
    18661 accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga tgccggtgat
    18721 acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct cttcctgttt
    18781 caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact ccacaaacag
    18841 ggttgccatc ggtttgccga tgttgtcctt agtgaagtta gacatgatgt taccaccagc
    18901 gctatcgagc gagatgttaa cctgcggttg gttgtattcg tcctggctgg aagtggagtc
    18961 ggtgatatgg tcaccggtca gaattacgcg tttgtacagc acaactggct gaccttcgcg
    19021 ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg cctggtcaac
    19081 gttggtgttt accagacgga attccagcgt tgccgtcgca cccagaatct ctttcgcacg
    19141 cgcagtgtcc tgaatacctg gcagttcaac aacgatacgg tcagcaccct gacgctgaac
    19201 caccggctcc gctacgccaa gttggtttac acggttacgc aggatattga tgttctgctg
    19261 caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac gcagctggtt
    19321 gctgccctgg ctactaatca ccaggtccgg atggcgcttg ctcagatacg caatggcttc
    19381 atcacgagct ttagcatcgc ggaaagtaat gctcaggccg tagttgtttt ctttacgaac
    19441 ggtggtatac gggatgccct tttcacgcag atcgctgcgc aggctatcga tattttgttc
    19501 ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa cgccgccacg
    19561 caggtcaagg ccgagcttca tcggctcagc gtgaatagct gccagccagc gcggcgttgc
    19621 cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta atgcttcacg
    19681 agcgcgcagc tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct cttccagtgc
    19741 cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga tcagcgtttg
    19801 ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct caccaaacag
    19861 gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga cgtacttcca
    19921 caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt acagcgcctt
    19981 catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta cttcagtggt
    20041 gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc gaccaaccag
    20101 gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct ttttgtgttc
    20161 tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac cgaataccac
    20221 cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg ccgctaccgc
    20281 atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca acgttcaaag
    20341 gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct aatttaccct
    20401 cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg ttatgaatgg
    20461 tgttgagtcg cgcgcctaat atttcgttgc aacggtcaag atgatgcaag taagcgcgtg
    20521 aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta tcgctcttat
    20581 acttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca tttcgggcgt
    20641 tgcgggttgg cattacgcag tcaaacatat cgataccacg acgtacgcct tcaaccaggt
    20701 cttctggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt tgcgggcata
    20761 catgctccag aatgcggtgc atatctgctt tcggctcacc cacagccaga ccgccgacag
    20821 cgtagccatc aaaaccgata tctaccagac ctttaacaga aatatcacgt aaatcttcgt
    20881 aaacgctgcc ctgaatgata ccaaacagcg catttttgtt tccgagactg tcaaaacgct
    20941 cacggctacg cttcgcccaa cgcagagaca tctccatgga gcgttttgcg taatcccagt
    21001 cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca agatcgtact
    21061 gaatctccat tgatttttca ggatcgagga aaatcggatc gccgttgatc gggttacgga
    21121 agtgcacgcc ctgttcggtg attttacgaa tatcgccaag gctgaagacc tggaagcccc
    21181 cggagtcggt gaggatcggc cccttccact gcataaaatc gtgcagatcg ccgtgcagtt
    21241 tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg ataatttgcg
    21301 cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag gtgccaacag
    21361 gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca cggcgtgcgc
    21421 gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag aaaaacagtc
    21481 caacgtttta aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga
    21541 ttgtacgtaa taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgct
    21601 gctttatagg cgttcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc
    21661 gtcgactctg gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc
    21721 ggatagataa agatttgggt atcgtcgaag aacggttcaa tgagatcgtt tttcgctgcc
    21781 tgagccgcgc tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg
    21841 cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag
    21901 tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg
    21961 tgcaacgtca caaacgccat ctccacgcct ttggcgcgca atttttccag caaaggctcg
    22021 tcaaaatgca gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt
    22081 tgataaagtt cgcggtcagc gtcttcgtcc ggacggtcga tatacggcgg cagcggcata
    22141 tgcccgatac tgttgaggat atccagtacc gaacgatcat cattgaattc gacttcaaac
    22201 agtgcgccgt ggcgcgcggt cattgttgcg ttaatacttt cgtcatcgcc cagcagcagt
    22261 tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg
    22321 agcatccgct caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacaggcgc
    22381 gccgggatca cgctagtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt
    22441 aaatcggtga aagtaccgtg cgtcagcgcg cccgtaggcc cgtccagcga cagtaaacga
    22501 cagctactgc gttcaggcat ggggtagtgg gcaatcaggg attcgggcaa ttcaaagaag
    22561 aaatcggtaa cgcgcatgac actgactcag actaaaataa gaggcgggta gtctagtgcc
    22621 ggggcgctct ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt
    22681 agctcacctg catttagccc atctcgcgga aagctcgctt tccggcaatt tactggctga
    22741 tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat
    22801 gcatcgacgt atcgacgtat tgactgacaa tctgccggaa gtccgcgaag cacgggagtg
    22861 gtttcgtcgt gaaacgcgcc gcgttgcgcc tattacgctg gatgtcatgt gggatcactt
    22921 tctttcccgc cactggtcgc agctgtcgcc ggactttccg ctacaggaat ttatctgtta
    22981 tgcccgcaag caagtgatga cgattttgcc ggactcaccg ccacgtttta tcaatctaaa
    23041 caattacttg tggtcagagc agtggctggt gcgctatcgc gatatggatt tcatccagaa
    23101 cgtgttaaac ggcatggcaa gccgtcgccc acgtctggat gccctgcgtg actcctggta
    23161 cgatttaaac gcccattata ccgccctcgg aacccgcttc tggcagtttt atccgcggat
    23221 gatggcgcag gcgtcacaca aggcgttata aaacgttagt tcatccatac cgtagccgaa
    23281 atggcaggca aagccagaat cccgtcagtc agttgcccat gcccttcttt gcattgccat
    23341 tgcacggcat tgagaaacgg tgacgcgggt agcaccactt cacaggcctc gccacggttg
    23401 attgccacca gtacacgttg ctgattcagc acgcggacaa ataccactac gttatcttcc
    23461 gcatacagca cctgacagcc gccacgacgc agcgcctgac ttttcttacg cagcacaatc
    23521 attcgccggt acagcgcgaa taacgccgta tcctgctttt ccacctgcca ggggaacggt
    23581 ttacggcaaa acggatcgtt tttaccatcc agtcccactt catcaccgta ataaatgcac
    23641 ggtacaccag gccaggtgaa cagccagacc accgccagcg gcaggcgcgc aatatcccga
    23701 ccgagcagcg ttttaaatcg cgcagtatcg tggctgtcga gctgattaaa catacgtaat
    23761 tgttgttgat gagaaagccc tgcgcggtaa ttatccatcc aggccataca ggtttgggca
    23821 tcaatttgct gcggatcgta agagatatcg gtattggcaa gaaatcccca caacgggaat
    23881 gtgaagccac gatagttcat ggcggcatct tccacatcgg cctgtaacca ttgccgtgca
    23941 tcgccaaaat gttcgccgac aatatacgct tccggctggg tttctttcgc cgcttcggtg
    24001 atcccagcaa cgtgctgcat attattgcgc gccccacccg cctcccccag catatgcacc
    24061 acatccagcc gccagccgtc catactccac ggcgctgtca gccaatggcg gacaatactg
    24121 tcttccccgc gataaatttc attcaccaga ctttccgact gataatccag cttcggcaag
    24181 ctggcatagc caagccagtc gagcgccgtg ccatcatcac taaacgagta ccagtcgcgc
    24241 cagggcgatt cagggttgta acaagctccc cccgtgccac gattgtgcct gtcaaaccag
    24301 gcatgggaat cgccactgtg gttaaacacg ccgtccagca ccagccgcat tcccagctgc
    24361 tgcgtattgt gtcgcaaacg cagcaacgcc ccatcaccgc caaactgcgg atcgacatgg
    24421 cgataatcct cggtatcgta ttgatgtacg ctgggagctt taaacaccgg attgagatac
    24481 agcgctgtca cgccaagctt tttcagatac ggcagttttt cgcttatccc gtccagatcg
    24541 ccgccataga acgttgatcc gcccgcctgc gccgtgaccg gttcatccca gtcacgcaag
    24601 atgatctctt gtccggctgc atgatggtaa tagacatgat cctgttcagc ttcacgagga
    24661 agactacgcg caaaacgatc agggaagatc tgataaaaaa tctgatccgc agcccattgt
    24721 gggccgatat ccggtacatc gacggcaaac tgctccagtc gtgccggcgg cattcggctg
    24781 aagccctgcg gtgacgcctg gctgcggctg actgcgctgc ttatgcattg gtactgacat
    24841 ttcttcgtta tcgtgttctg tacgcagcat aatgcgctgc ggtgggtctt cgcccgtcag
    24901 ccacagtgta atgagcagtt gatctttgct ttgtttaaca aatgggggca ccggcaggtg
    24961 ccatgcattt aacatcataa ttcccctgtg atgaaaatct gatcaccttg ccatagccta
    25021 atgcaggatc actcatcatc tgaaggatat ttgggggagg aggacggggg cggataactt
    25081 tcggaatagc cggatcagga tttcctcctg aaccggctta cagaacgcaa ggattactgg
    25141 ttttcagcca gctgacgatc gtggcgacgc ttaaacatcc agccaatcaa cagcacaaca
    25201 atccacgcga aaccgacata cagcgagata cgcgtatccg ggtgataacc aatcaacccg
    25261 ataataaaga gcaggaaaat cagcccgccg atggtcgttg ctaccccacc cggcacttta
    25321 aatttcagcg ccttaacttc ttctggcggc aaacggcgac ggaaggcgat ttgcgacagc
    25381 aggatcataa tccacaccca taccgtggcg aaggttgcca gcgaagcgat caccaggaag
    25441 acgttttccg gcatgatgta gttcagatac accgcaaaca gcagcgcggt agtcatcacc
    25501 agcaccgtaa cccacggaat accgcgacgc gacgttttgc tgaaaatttt cggtgcgctg
    25561 ccctgctctg ccataccgtg gagcatacgg cctacgccaa atacgtcact gttaattgcc
    25621 gacagcgaag cggtcagcac aacaaagtta agaatgctgg cggcaaaggt aatgcccata
    25681 tgctggaacg tcagcacgaa cgggctaccg gcagtgccaa cctgattcca cgggtagata
    25741 gacataatga cgaacagcgt accgacgtag aacaccagaa tacgcatcgg cacggagtta
    25801 atcgcacgcg gaatcgattt ctcaggatct ttcgcttcac cggcggtaat cccgataatt
    25861 tcgatcccac cgtaagcaaa catcaccatt tgcaacgaca ttaccatgcc aagccagccg
    25921 ttactgaaga agccgccgtt gctccacagg ttatgaatac cggtcggttg cccgccgttg
    25981 ccaatccccc agatgatgat gccgaaaccg gcgacaatca tgatgatgat ggtggcgact
    26041 ttaaagaacg agaaccagaa ttccagctca ccgaatacct tcacgctcat caggtttacg
    26101 gcgcaaatga tcagcaccac gctcagtacc caaatccagt gcggcaccgt cgggaaccag
    26161 acacccatat agataccaaa agcggtcaca tcggcgatgg cgacaattag gatttcaaag
    26221 cagtaggtcc agccggtaat gtaacctgcc agcgggccga ggttttcctg cgcataacgc
    26281 gagaaagagc tggcggccgg gttatgtacc gacatttccc ccagcgcacg cataatgata
    26341 tacgccgcga taccaccgat aatataggcc aacaacacgc tcggaccggc cattttgatg
    26401 gcgtctgccg aaccgtaaaa cagcccggtg ccaattgctg aacccagtgc cataaagcgt
    26461 atgtggcggg tacttagccc acgctttagc ttgttcttac tttccatcaa ttcctaaccc
    26521 atcaacacaa taaaaaacca cggggcatcg cagccccgtg gttaaaacaa atgttcagtg
    26581 atttagtgag cgctggaggt cacctgacga cctgccgcgc gatcccagat aatggccaga
    26641 accaccatca ccactgttgg cattaaccac gccaggcctt gttcggccag cggtaaacgc
    26701 tgcgcccagg acggtaagat atcgctgaat gcagatgctt tgatcccgtc gagaatacca
    26761 aaaagcaggc tgataaacat cggcggggca atcacgcggg acgaattatg ccaccatgag
    26821 cgtgtaaaac ttaatacaac cagtgcgata cacggcggat aaatagcggt cagcaccggt
    26881 acggagatct ggatcagctg gcttaagccg aggttagaca ccaccatcga gaagccgccg
    26941 aggataaaca ccagcgtacg ataagagagc ggtacgtact gggcaaagaa ttctgcacaa
    27001 gcacaggtca ggccaactgc cgttaccagg caggcgatga agattaacgc cgccagcagg
    27061 aagctaccgc cgccgccaaa tgtgtgctga acgtaagcat gcagaatagc agcgccgttt
    27121 gcagactgat cgaccagcga cgcgctgtct gaacccagac ggaacagcgc caggtacagc
    27181 agagtcagac caacacccgc catcaggcca gcccagacgg tataacgggt cagcagacgc
    27241 gcttcggtaa cgccacgaga acgcgccgcg ttaacaataa cgataccaaa caccattgcg
    27301 cccagcgtat ccatggtcag atagccgtta acgaagccgt tagaaaacgc agcgttttga
    27361 taagcctcag tcgccgtgct gatagaaccc gccggccaga caatagcggc aacagacagg
    27421 atgaccagcg cgataatttt cagcggcgca aggaagttgc ccacggtatc cagcagcttg
    27481 cccggataga gcgaaaccag aataacgata gcgaaataga ccaggctgta gataaacagc
    27541 ggcagcgcgg aatcacccgt cagcggcgca atccccactt caaaggaaac ggtagctgta
    27601 cgcggcgtag cgaaaagcgg ccccaccgcc aagtaacaaa ccgttgccag cagcacgcca
    27661 gcgactttac cgattggcgt gctgaggctg tcaacaccgc cgccaacttt tgccagcgcc
    27721 actaccgtta ataccggcag gccaacggca gtaatgagga agccgaatgc cgcagtccag
    27781 acgtgttcgc ctgcctgcaa gccgaccatt ggagggaaaa taatgttacc tgcgccgacg
    27841 aacaacgcaa atgtcataaa gcccagagcg atgatatcgc gcgatcttaa ttgatgggtc
    27901 ataaatcata ctgcctgtgg atgtggtgtt gtaacgttaa atttttgtgc caatccctgg
    27961 ggatgataca gcggtatatt taaacaattt tagcgggaaa tactcccaac ctggcgcggc
    28021 gaagaatagt ttttcgattt accgcgttaa tgcagtcagt atgacagcac ctgaagcgca
    28081 atttaaacgc ttataacgtt ttaaagcaag gagggatcta aaaaccagag gtatatagcg
    28141 ataagaaatc accaaccagt ataaaacact atttccgtga aaaagacatg gctaatcatg
    28201 cgaacaaaaa atcaccaacc gttgtaaaat ctaacgcccg tattttgact gcaaagaaaa
    28261 taagccagca tcgactggct tatggcaata aaagatgaca aaggcggatt aatcgctgtt
    28321 tttggcaatt aaacgttccg ggataacaaa actgaaacgt gttccttttc ctactgtact
    28381 ctcaatattc aggcgacttt cgtgatgatt cacagcatgt ttcacgatcg ctaaccctaa
    28441 tccgctaccg ccggtttgcc gggaacgcgc tttatcaacg cgataaaaac gctcggtcag
    28501 gcgcggaata tgctccggtg caatgcccgg tccgttatct tcaacgctaa attcggcacc
    28561 gtgcggcact cgctgccagc gtacggtgat atgcgtgcct tctggagtat gattcacggc
    28621 gttatagacc aggttcgaaa tcgcactgcg tagctgatct tcgttgccag acactttgag
    28681 gccgttatct atctcaaagg taaatgtctg ttttttctga ctcagagtct gagcctcgcg
    28741 ctcaacaacg cgcagcatca tcggcacatc aaccttttca ttgagcaatt gcgtcggcgc
    28801 ggcttcaatt ttcgacagcg ttagcaactg cttcaccagc ccttccatcc gctgggtctg
    28861 ctcgcgcatg gtgtgcaacg ctttttcgcg taccgcgcct tccagcggct gctcatccat
    28921 catctccagg taaccctgta acacggtcaa tggcgtacgt aactcatggc tcacgttggc
    28981 aaaaaagtta cgccgcgccc cttccagttg atgcatttgc gtgacatcac gcgccaccat
    29041 cagcaactgt ttgtgggtat aaggcatgac gcgaatttcc agatgccgcc cggtgttgag
    29101 caccagattg agcgggcgag aaaaatcacg cgttttcaga tattgcgtaa actccgggta
    29161 acgcagtagg ttaaggatgt tctgcccgtt atcttccggc cagcgcaaac caagaatttg
    29221 ttgcgccaga ccgttacacc agaaaatacc gccctcttcc gtggtcagca ccaccgcgtc
    29281 gggcagcgac tccgcgccgc tacgaaagcg tttaatcaga ttgcccagtt cacggcggcg
    29341 ttttttattt cgcagctgca tctggtgtaa gccgtatagt agcggttccc agctaccacg
    29401 ccccggtggc ggggtcatac tgcgatccac ccacaaccac catgaaaggc gcaataaatt
    29461 ccagaaatgc cagataagca gtcctgttac cgatgccagc aaaaaccagg gcaggtaacc
    29521 aaaaaatgca cccaggatga aagccgggag gcaacaaagt agcagctcca gcaccagcct
    29581 tttccacgac agccgttcca gcacgtaaga tactccagtt aagaaatcat aagccctgct
    29641 ctgcgtccga tgagcaaggc gttaaaagcg ggttgaaaaa cgatatcctg taccgcgcac
    29701 ggtctgcacc atgcggtcat gcccgccggg ctccagtgct ttacgcaggc gacgaatgtg
    29761 gacatcgacc gtgcggtctt ccacatacac gttagttccc cagacgtggt ttaacagctg
    29821 ctcgcggctg tacacgcgct caggatgcgt cataaaaaag tgcagcagtt taaattctgt
    29881 cggccccatc tccagcggct cttcgcccgc catcactcgg tgagatgtcg ggtcgagact
    29941 taatccctgc atctcaatca cctcttccac cgccattggc gaaatacggc gcattaccgc
    30001 tttgattcgc gccaccagct ccttcggcga aaacggcttg gtgatatagt catccgcgcc
    30061 ggtttcaagg ccgcgcacgc gatcttcttc ttcccctctg gcggtcaaca tcaccactgg
    30121 aatatcccgg gtcatcgact cgcgcttgag gtgtttgatg aactggatac cggagccgcc
    30181 aggtaacatc cagtcaagga gaattaaatc cggccagggt tcattcagtt gattcacagc
    30241 actgtcataa tcttccgctt cgaccggctg aaagccattt tgttcgagca cgaagcagac
    30301 catttcgcga attggagctt catcttctac gaccagaata cgtctcgcca tgatttgccc
    30361 tgttgtaata aataggttgc gatcattaat gcgacgtcat tatgcgtcag atttatgaca
    30421 gatttatgaa aagctcgtcg cacatatctt caggttattg atttccgtgg cgcagaaaaa
    30481 agcaaatggc acatctgttt gggtataatc gcgaccatgc tttttcgcca gggaaccgtt
    30541 atgcgcatcc ttcacacctc agactggcat ctcggccaga acttctacag taaaagccgc
    30601 gaagctgaac atcaggcttt tcttgactgg ctgctggaga cagcacaaac ccatcaggtg
    30661 gatgcgatta ttgttgctgg tgatgttttc gataccggct cgccgcccag ttacgcccgc
    30721 acgttataca accgttttgt tgtcaattta cagcaaactg gctgtcatct ggtggtactg
    30781 gcaggaaacc atgactcggt cgccacgctg aatgaatcgc gcgatatcat ggcgttcctc
    30841 aatacaaccg tggtcgccag cgccggacat gcgccgcaaa tcttgcctcg tcgcgacggg
    30901 acgccaggcg cagtgctgtg ccccattccg tttttacgcc cgcgtgacat tatttccagc
    30961 caggcggggc ttaacggtat tgaaaaacag cagcatttac tggcagcgat taccgattat
    31021 taccaacaac actatgccga tgcctgcaaa ctgcgcggcg atcagcctct gcccatcatc
    31081 gccacgggac atttaacgac cgtgggtgcc agtaaaagtg acgccgtgcg tgacatttat
    31141 attggcacgc tggacgcgtt tccggcacaa aactttccac cagccgacta catcgcgctc
    31201 gggcatattc accgcgcaca gattattggc ggcatggaac atgttcgcta ttgcggttcc
    31261 cccattccac tgagttttga tgaatgcggt aagagtaaat atgtccatct ggtgacattt
    31321 tcaaacggca aattagagag cgtggaaaac ctgaacgtac cggtaactca acccatggcg
    31381 gtgctgaaag gcgatctggc gtcgattacc gcacagctgg aacagtggcg cgatgtatcg
    31441 caggagccac ctgtctggct ggatatcgaa atcactactg atgagtatct gcatgatatt
    31501 cagcgcaaaa tccaggcatt agccgaatca ttgcctgtcg aagtattgct ggtacgtcgg
    31561 agtcgtgaac agcgcgagcg tgtgttagcc agccaacagc gtgaaaccct cagcgaactc
    31621 agcgtcgaag aggtgttcaa tcgccgtctg gcactggaag aactggatga atcgcagcag
    31681 caacgtctgc agcatctttt caccacgacg ttgcataccc tcgccggaga acacgaagca
    31741 tgaaaattct cagcctgcgc ctgaaaaacc tgaactcatt aaaaggcgaa tggaagattg
    31801 atttcacccg cgagccgttc gccagcaacg ggctgtttgc cattaccggc ccaaccggtg
    31861 cggggaaaac caccctgctg gacgccattt gtctggcgct gtatcacgaa actccgcgtc
    31921 tctctaacgt ttcacaatcg caaaatgatc tcatgacccg cgataccgcc gaatgtctgg
    31981 cggaggtgga gtttgaagtg aaaggtgaag cgtaccgtgc attctggagc cagaatcggg
    32041 cgcgtaacca acccgacggt aatttgcagg tgccacgcgt agagctggcg cgctgcgccg
    32101 acggcaaaat tctcgccgac aaagtgaaag ataagctgga actgacagcg acgttaaccg
    32161 ggctggatta cgggcgcttc acccgttcga tgctgctttc gcaggggcaa tttgctgcct
    32221 tcctgaatgc caaacccaaa gaacgcgcgg aattgctcga ggagttaacc ggcactgaaa
    32281 tctacgggca aatctcggcg atggtttttg agcagcacaa atcggcccgc acagagctgg
    32341 agaagctgca agcgcaggtc agcggcgtcg cgttgctcac gccggaacaa gtgcaatcgc
    32401 tgacagcgag tttgcaggta cttactgacg aagaaaaaca gttacttacc gcgcagcagc
    32461 aagaacaaca atcactaaac tggttaacgc gtctggacga attgcagcaa gaagccagcc
    32521 gccgtcagca ggccttgcaa caggcgttag ccgaagaaga aaaagcgcaa cctcaactgg
    32581 cggcgctcag tctggcacaa ccggcacgaa atcttcgtcc gcactgggaa cgcatcgcag
    32641 aacacagcgc ggcgctggcg catactcgcc agcagattga agaagtaaat actcgcttac
    32701 agagcacaat ggcgcttcgc gcgagcattc gccaccacgc ggcgaagcag tcagcagaat
    32761 tacagcagca gcaacaaagc ctgaatacct ggttacagga acacgaccgc ttccgtcagt
    32821 ggaacaacga actggcgggt tggcgtgcgc agttctccca acaaaccagc gatcgcgagc
    32881 atctgcggca atggcagcaa cagttaaccc atgctgagca aaaacttaat gcgcttgcgg
    32941 cgatcacgtt gacgttaacc gccgatgaag ttgctaccgc cctggcgcaa catgctgagc
    33001 aacgcccact gcgtcagcgc ctggtcgcgc tgcatgggca gattgttccc caacaaaaac
    33061 gtctggcgca gttaatggtc actatccaga atggatctct ggaacagacg caacgtaatg
    33121 tcgcacttaa cgaaatgcgc cagcgttata aagaaaagac gcagcaactt gccgatgtga
    33181 aaaccatttg cgagcaggaa gcgcgcatca aaacgctgga agcccagcgt gcacagttac
    33241 aggcgggtca gccttgtcca ctttgtggtt ccaccagcca cccggcggtc aaggcgtatc
    33301 aggcgctgga gcctggtgtt aatcagtctc gattactggc gctggaaaac gaagttaaaa
    33361 agctcggcga agaaggtgcg gcgctgcgcg ggcaactgga tgcattaacg aagcagcttc
    33421 agcgcgatga aaacgaagcg caaagcctcc gacaagatga gcaagcactt actcaacaat
    33481 ggcaagccgt cacggccagc ctcaatatca ccttgcagcc acaggacgat attcaaccgt
    33541 ggctggatgc acaagatgag cacaaacgcc agctgcggtt actcagccaa cgacatgaat
    33601 tacaagggca gattgccgcg cataatcagc aaattatcca gtatcaacag caaattgaac
    33661 aacgccagca gcagctctta acggcattgg cgggttatgc actgacattg ccacaggaag
    33721 atgaagaaga gagctggttg gcgacacgtc agcaagaagc gcagagctgg cagcaacgcc
    33781 agaacgaatt aaccacgctg caaaaccgta ttcagcagct gacgccgatt ctggaaacgt
    33841 tgccgcaaag tgatgatctc ccgcacagcg aagaaactgt ggcgctggat aactggcggc
    33901 aggttcatga acaatgtctc gcattacaca gccagcagca gacgttacag caacaggatg
    33961 ttctggcggc gcaaagtctg caaaaagccc aggcgcagtt tgacaccgcg ctacaggcca
    34021 gcgtctttga cgatcagcag gcgttccttg cggcgctaat ggatgaacaa acactaacgc
    34081 agctggaaca gctcaagcag aatctggaaa accagcgccg tcaggcgcaa acgctggtca
    34141 ctcagacagc agaaacgctg gcacagcatc aacaacaccg acctgacggg ttggctctca
    34201 ctgtgacggt ggagcagatt cagcaagagt tagcgcaaac tcaccaaaag ttgcgtgaaa
    34261 acaccacgag tcaaggcgag attcgccagc agctgaagca ggatgcagat aaccgtcagc
    34321 aacaacaaac cttaatgcag caaattgctc aaatgacgca gcaggttgag gactggggat
    34381 atctgaattc gctaataggt tccaaagagg gcgataaatt ccgcaagttt gcccaggggc
    34441 tgacgctgga taatttagtc catctcgcta atcagcaact tacccggctg cacgggcgct
    34501 atctgttaca gcgcaaagcc agcgaggcgc tggaagtcga ggttgttgat acctggcagg
    34561 cagatgcggt acgcgatacc cgtacccttt ccggcggcga aagtttcctc gttagtctgg
    34621 cgctggcgct ggcgctttcg gatctggtca gccataaaac acgtattgac tcgctgttcc
    34681 ttgatgaagg ttttggcacg ctggatagcg aaacgctgga taccgccctt gatgcgctgg
    34741 atgccctgaa cgccagtggc aaaaccatcg gtgtgattag ccacgtagaa gcgatgaaag
    34801 agcgtattcc ggtgcagatc aaagtgaaaa agatcaacgg cctgggctac agcaaactgg
    34861 aaagtacgtt tgcagtgaaa taactattca gcaggataat gaatacagag gggcgaatta
    34921 tctcttggcc ttgctggtcg ttatcctgca agctatcact ttattggcta cggtgattgg
    34981 tagccgttct ggtggttgtg atggtggtat gaaaaaagtc attttatctt tggctctggg
    35041 cacgtttggt ttggggatgg ccgaatttgg cattatgggc gtgctcacgg agctggcgca
    35101 taacgtagga atttcgattc ctgctgccgg gcatatgatc tcgtattatg cactgggagt
    35161 ggtggtcggt gcgccaatca tcgcactctt ttccagccgc tactcactca aacatatctt
    35221 gttgtttctg gtggcgttgt gcgtcattgg caacgccatg ttcacgctct cttcgtctta
    35281 cctgatgctc gccattggtc ggctggtatc cggctttccg catggcgcat tttttggcgt
    35341 cggcgcgatc gtgttatcaa aaattatcaa acccggaaaa gtcaccgccg ccgtggcggg
    35401 gatggtttcc gggatgacag tcgccaattt gctgggcatt ccgctgggaa cgtatttaag
    35461 tcaggaattt agctggcgtt acaccttttt attgatcgct gtttttaata ttgcggtgat
    35521 ggcatcggtc tatttttggg tgccggatat tcgcgacgag gcgaaaggaa agctgcgcga
    35581 acaatttcac tttttgcgca gcccggcccc gtggttaatt ttcgccgcca ccatgtttgg
    35641 caacgcaggt gtgtttgcct ggttcagcta cgtaaagcca tacatgatgt ttatttccgg
    35701 tttttcggaa acggcgatga cctttattat gatgttagtt gggctaggga tggtgctggg
    35761 aaatatgcta agtggcagga tttcaggacg ttattcacca ctgcgcattg cagcagtgac
    35821 tgactttata attgtactgg cactgctgat gttctttttc tgcggcggca tgaaaacaac
    35881 gtcgcttatt tttgctttta tttgttgcgc gggattattt gccctttcag caccgctaca
    35941 aatattgtta ctacaaaacg ccaaaggcgg agagttatta ggtgccgcag gtgggcaaat
    36001 agcgtttaac ctcggtagcg ccgtcggcgc atattgcggt ggtatgatgc tgacgctggg
    36061 gctggcatat aattacgtgg cgctgcctgc cgccctgctt tcgtttgctg cgatgtcgtc
    36121 gttgctgctg tatggtcgct ataagcgcca gcaagcggcg gatactccgg tgcttgctgg
    36181 cgaaaccact ggggtaggtt atagtctcgg tgttcattac ttattgccgg atgcggcgtg
    36241 aacgccttat ccgccctacg cggttctggc acattttgca ggcctgataa gacgcggcaa
    36301 gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg gccataacca
    36361 cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttacgcaccg gcgtttcaca
    36421 ttcgccgccg aagacaaatt gtttaatcaa ctgcggaacc gtttgatata aacggtctac
    36481 attgctcatc cccccccccc caggacaatc acatccggat cgagaatatt cacgacatgt
    36541 gccagcgatt ttgccagccg cagctcgtag cgacgcaatg ccagttccgc taccggatcg
    36601 ctttcttcaa ccaggcggat aatttcactg cctttcagcg catgtccgct caaacgacga
    36661 taatccatcg cgaatcccgt gcccgaaata aaggtttcaa tacaaccttg tttaccgcaa
    36721 taacaaggga cttcctcgcg ataacgcagt tcgtcttcgt ccatccacgg tagcggattg
    36781 tgtccccact cacctgccgt gccattgccg ctgatatgcg cccgcccatt gaatgccacg
    36841 cccgcgccgc atcccgtgcc gataatcacg gcaaataccg tctgcgctcc cgctgccgcg
    36901 ccatctactg cttctgaaac cgccagacag ttagcgtcat ttgccagccg cacttcccgc
    36961 tgcaacctcg cgcttaagtc tttatcgaat ggctgaccgt tgagccaggt tgaattggca
    37021 ttcttcacca caccggtgta aggcgaaatt gagccaggaa tgcccatacc taccgttccg
    37081 cgctgccccg tcgcctgctc cgccatatca accaacgtgg cgatcgtttc aatagtctgc
    37141 cggtaatcat cacgcggcgt gggcagacga tggcggtaca actgctcccc tgcatcgccc
    37201 agtgcaatca cttcagtttt ggtgccgcct aaatcgatac ctatacgcac ggtactctcc
    37261 ttattttttt caatatcaat agcgtagaga cggacaaccg gattggcaat gcaaggccgc
    37321 cgacaattcg ttatcatgcc cgctaaattt aacaacaagg ccgtggaaat tatcatgctg
    37381 tggttcaaaa atttaatggt ttaccgtctt agccgcgaga tttcgctgcg tgcagaagag
    37441 atggaaaaac agctagcctc gatggcattt accccatgcg gcagccagga catggcgaag
    37501 atgggctggg ttcctccgat gggatcgcac agcgatgcgt taacgcacgt tgccaatggt
    37561 caaattgtta tctgcgcgcg caaagaagaa aaaatcctcc cgtctccggt gattaagcag
    37621 gcgctggaag cgaaaatcgc caaactggaa gcggaacagg cgcgtaagct gaagaaaacc
    37681 gaaaaagatt cgctgaaaga cgaagtgctg cactctctgt tgccgcgtgc tttcagccgt
    37741 tttagccaga caatgatgtg gatcgacacg gttaacggtt tgattatggt ggactgcgcc
    37801 agtgccaaaa aagcggaaga tacgctggca ctgctgcgta aaagcctggg gtcgttacct
    37861 gtggtaccat tgagcatgga aaacccgatt gaactgacgc tgaccgaatg ggttcgctcc
    37921 ggtagcgcgg cacagggctt ccagctgctt gatgaagccg agctgaaatc gttgctggaa
    37981 gatggcggcg tgatccgcgc gaagaaacaa gatctgacca gcgaagagat caccaatcac
    38041 atcgaagccg gaaaagtggt gactaaactg gcgctcgact ggcagcagcg cattcagttt
    38101 gtgatgtgcg acgatggttc gctcaagcgt ctgaagtttt gcgacgagct gcgcgatcaa
    38161 aacgaagata tcgaccgtga agatttcgcc cagcgttttg atgccgattt catcctgatg
    38221 actggtgaac tggcagcgtt aattcaaaac ctgattgaag gattaggtgg cgaagcacaa
    38281 cgttaattgc tgattttcct ttaatgccgg atgcgacgcc tgccgcgtct tatccggcgt
    38341 acgaagccac accagggata taattattcg ctacggcgga caataatttt tagcgcagca
    38401 atattatgcg ttttatgctg taacttgctc catggacgtt gtgtcattgt ttttcctcaa
    38461 gccagtgacg cgcttcactg cgaattttcc gcattgcccc ggttttcact aaatcccgta
    38521 acagcgaaag atcgggttgt tcgacaaata agagtaatga tggatcctct tttaaccacg
    38581 tcgttttcac atcggcagcg agctgtggat tattcatagc tagcgaccga tgccgagact
    38641 cggtcaatgt cgttaacaac gacgccgggg tatgtgcgtt gcgtaatgcc gtttggcgca
    38701 aatgatcatt gcgactttgc agcaatgcac gccaccattc atcatctgat ttatctttta
    38761 tcgtagccag ttccgcaaca atatctggag atcttcactg atatttatat caaccttgat
    38821 gaattatgaa tatatcggaa attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta
    38881 aagagaggat atttcagaaa aatgccctcc cgttcaaggg gaaggcgagg aattacagat
    38941 agcggcacag ataagaggtg ggttcggcaa cttgcagatg aaactcactg tgaccgggaa
    39001 cattaaacac cgaaccggct tcatacacct gccagtcggt cgcatcaggc agtaacacat
    39061 tcagcgcgcc actgattacc gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc
    39121 cttcaaccat aacacccacg ctggcgcgac cagtgctgct gctggaaaag ccgattgatt
    39181 tcactttgcc ggaaaagtac tcattacttt gaagcataaa ctggccctgt ctggtagttg
    39241 aacatctact tcactatagg ggccagaggc gctggctgtc acgcaaaatt acaccagtaa
    39301 ttccgcagcc agccgtgcaa tcaatacgtt agacagcaag acaggaacat cgagctgttt
    39361 ttgcagtaaa tcgcgatgac gttggtgaaa tcctaaacaa tccagcatga tgacatcagc
    39421 cccttttgcc agtaattctt tccctgcatc aatgattttt tgttctgaat catgaatggg
    39481 gttacccaat gaaaataccg gcgatttctg caaaatttgc catttttgcg cctgaacggg
    39541 cagcatctcc tcaaccggaa cgataacccc cacctgatga tcttcaacaa tagaggaaac
    39601 cagtggaggc aatattcgcg acggctcaag aaagatcgta ttacgcgcag tcatactact
    39661 aatgtttgct gtactcatta ataaaatgac gtcataaccc tgattatcga gcacttcaac
    39721 cacaccttgc aggtctcgct ccactttacg acgcgaaaca tgggccagct ggttgtcatt
    39781 taataatgtg agaatggtgt cttcgcctgc ttctggcgcg tactccgcca tcacttcttc
    39841 acgacttaac ttccccagca ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt
    39901 caggagcggc aaaacctcct gcataggtac aatgccgatg gtcaggatcg ccaacgacgc
    39961 actcattgtt catccacctt acttttcttt cgtcgttaat taccgggcaa gtgtgaagca
    40021 ccactgctga cacttacttg tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg
    40081 cataatgtaa agattccaaa tgattccagt aatggattgt tattgtttaa tattctaatt
    40141 attagaaaaa catgaattat gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat
    40201 cccttccgag cagtctgatg cggtcactct ttgtcagggt cctcatagcg ttttttcgca
    40261 tccatcgctt cctgagcggt cggatgttca ctgatcaacg agtctggttt aggatgatcg
    40321 gctctgagtt ggtaccaggt taccgtctgt cctggctttc ctttttcaat cgtcactata
    40381 tatgcttcac gaggataagg cggtttcgtt ggcatcgttc ttccttattt cacggaatga
    40441 acgttaagta taggcgctct aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat
    40501 ttcagaaatc acctggctgg gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg
    40561 atatagcgca tcgcgttctt ccagcacttc ctgaacttct tcgctcagcg gttttcccgt
    40621 taaggttggc cgtaaatctt cttccggtgc agcttgcagt cggttaacca ggactgatac
    40681 tggcgcacac aaataaacca cgatcccgtt attttgcatg aagtgacgat taaattccgt
    40741 cagaataatg ccgccgcctg tagcgataac ggtggatggc gcagttaccg cttccagcgc
    40801 cgccgtttct ctggcgcgaa atcccgccca ctcttccctt tcgacgatct ccgcgaccgt
    40861 catattgagc tgtgattgca accactgatc ggtatcgaca aaccgacggt taagcgaatc
    40921 ggcaagggcc attccgaccg ttgttttacc acagccccga ggcccgatca gaaaaagagg
    40981 ttgtgtcatc gtgggttttc cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga
    41041 atagcgatca ttccatcaaa ctagtacaat ttcgattgta aagaaaaaat tccacttaaa
    41101 gtgaaaatct caatacatcc cttactatac caataaatat tcaagaatga agtgtaaata
    41161 ataaattaca tttagccacg actacgttgc acttccagcc accacttctc cagctccgcg
    41221 gcaaaggcct ggcggtcacg ttgtgaaagg ctatctggtc cgccggtctg gatcccactg
    41281 gcacgtaagg tatccataaa atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg
    41341 taacgttcgc cgcgcggatt aagcgccgct gcgcctttct cgatggcttc agcagccaaa
    41401 ggtatatctg cggtgatcac caaatcgccc gcttcacact gccggacaat ttcgttatcg
    41461 gcaacgtcga aacctgccgc gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt
    41521 aaactctggt ttgctaccag taccagcggc atctgcatac gttccgccgc gcgatacaaa
    41581 atctctttaa ttacattggg acacgcgtca gcatccaccc aaattgtcat aaagtcatcc
    41641 tttgttgggt aatcctctat tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc
    41701 ttctgccagc gattatcaaa acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa
    41761 aatcggtttt attggctgcg gcaatatggg aaaagccatt ctcggcggtc tgattgccag
    41821 cggtcaggtg cttccagggc aaatctgggt atacaccccc tccccgaata aagtcgccgc
    41881 cctgcatgac cagttcggca tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc
    41941 cgacatcatt tttgctgccg ttaaacctgg catcatgatt aaagtgctta gcgaaatcac
    42001 ctccagcctg aataaagact ctctggtcgt ttctattgct gcaggtgtca cgctcgacca
    42061 gcttgcccgc gcgctgggcc atgaccggaa aattatccgc gccatgccga acactcccgc
    42121 gctggttaat gccgggatga cctccgtaac gccaaacgcg ctggtaaccc cagaagatac
    42181 cgctgatgtg ctgaatattt tccgctgctt tggcgaagcg gaagtaattg ctgagccgat
    42241 gatccacccg gtagtcggtg tgagcggttc ttcgccagcc tacgtattta tgtttatcga
    42301 agcgatggcc gacgccgccg tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc
    42361 cgctcaggcg gtaatgggtt ccgcaaaaat ggtgctggaa acgggagaac atccgggggc
    42421 actgaaagat atggtctgct caccgggagg caccaccatt gaagcggtac gcgtactgga
    42481 agagaaaggc ttccgtgccg cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga
    42541 aaaactcagc aaatcctgat gactttcgcc tgacgtcagg ccgccacttc ggtgcggtta
    42601 cgtccggctt tctttgcttt gtaaagcgcc aaatctgccg atttcaacca ctcacgatag
    42661 tgactcattt gtgggttcag cggcgcaacc cccacactaa tccgtaaagt tacctgtggc
    42721 gtattcggca agcgtaacgt gtttagccct tcacgcaccc gtaacatggc ggtaatggcg
    42781 ctctcagctg gcgtaccgga catgattact gcaaactcat cgccgccaaa ccgaccaatc
    42841 acatcgctac cgcgcagggt aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg
    42901 cccacatcat gaccccaggt atcgttgatg ctcttgaaat ggtcgatatc gataatcagt
    42961 aacgttgcat cgcgattatg ccgccgacag ttatcaaatt cattgcgtaa catagtttcc
    43021 caatgacgtc ggttatacac gcccgtcatg ccgtcgcggg tactcatgac ctgcaacctg
    43081 cgtttatgtt ccgccagttt ggttgccgtc tggtagctga cccagccaaa cagcagagga
    43141 taaacgacaa taatgggaag ggagagccac cattccagcg gcgcactatt gaacgacacg
    43201 gtaatgcccg taagctcgag ggtgacaagg caggaaacca ccatcaacac cagacccgcg
    43261 acaaacagac gggggccgcc tgcccccatc aaattcagac acataatcat caacatagcg
    43321 gtggaaggca gcacgtttac gcccattacg cctacccaca ttcccgctaa tactgcatcg
    43381 gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg ccctgctcgc tatctgccag
    43441 gctaaatgcg gccagacgaa cgcccagccg accaacacta gccaccacca gcccggcggc
    43501 gggtgcgaaa ccagcgttga agcaatcggt aagaacatgc cagccaggcc aaccgcacgg
    43561 ggtagtctga cgcgacgggc gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt
    43621 aaaggaggtt cctccccatg cgccgccgct tttttgaaaa agttttcatc attcattatt
    43681 tttgggaaca ttcagaaaca attttcccaa attatagaga cggggctaaa ggcagaagta
    43741 tgaaatttcg ggtgagccac tttcgtagct cacccgttag cactatcagg tatcaggcgg
    43801 ctttcttgag gcaggcactc ataaacttat tacgatcatc acctttcaga gattgttgcg
    43861 ttgcttgatt attacattcg cgcatctttt gctgctgtgg cgtcaaactt ttttcaccag
    43921 gcgcagactt gctgttcttc aggcaatcac tcatgtaggt cttacgagca tcccctttca
    43981 acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg ctgttgttgt ggggttaacg
    44041 ttctctcggc agcgccgact gtggttaaaa aaaccagacc gaaaagcaag gtaaccagta
    44101 atgtaatttt catagcacca tccctcttca tgttttaacc atgagcgtat gcgcccgaga
    44161 tttgccatta agtctggttg ctaacagcaa aaaaaccacc cggcagcgaa aattcactgc
    44221 cgggcgcggt tttatttcag ccccagggcg gctttcatgg tgtagaagag atcggtctgg
    44281 tcggtcagtc caacgacatt ggcggcatgc gggccatacg ccgcaatacg caactgactg
    44341 ccggtatgtt cttgtgaatc ctcttcggag ttcccgtagc tcatcaccat cactgcgcca
    44401 tctttggtat ttagcgcctg ggtgaggccc ggagctttgg tatccggcgc gacaatctgg
    44461 ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt tgccatcctt tttagcgaat
    44521 tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct cgccaatttg cccacaagga
    44581 ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa cttgcaggaa aaatcctttc
    44641 tcatttttac tcaacaattc aatggctttg tcggtcatct gcgccagggt tggtacactg
    44701 tcattacgtt gcggattagg cgtacaggtg actgcgggct tatcgatatt gccgtggtac
    44761 gttgctttcg gtcctagcca gcgcactggc atattgccgt cagcaaacag tccaagcaga
    44821 ggtttttgct gattcgcttc cgtcaccgca tttagcgagg cggcatcgct gaccaactga
    44881 taaccacgcg cctgtgcctg ttcacgcagc gtttttccct gccattcacc ggcggttgcc
    44941 gtttcagcaa aggtttttgc gccaccgcca agcgtaacgt cggcacgagc attaagcagc
    45001 tgttcggtaa tcgatccttt tccgcctttt tccagagcgt tacccggaca tttttcactg
    45061 gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg ccaccagcgc agcgggcgtg
    45121 gcatcctgca actctgcggt agaaacgtta ccggtcgcca gacctgcggc ttttgccatt
    45181 tccagaatcg ttgggtgatc tttttcgtga atatcgacgc ccagcgcgcc gttataggtt
    45241 ttgacaccgg ttgaccaggc ggttgctgat gcagccgaat cggtgacgta gtccggtttg
    45301 ccggtttttt tattcagcgc atagtgagtg tattgcccgg taagcggtaa ggcatcgata
    45361 cctttaaaaa agccgcccgc accttcggca taattacgtg cggctgtaat ttccgaatcc
    45421 cccatcccat cgccaatcag caaaataata ttttttgcag gtttatcgct aagagaatca
    45481 cgcagagcgg cagtctgatc acccgttaaa cggcgagcac cgccgggtgc agtaatatcg
    45541 ccctgagcag cccggttttc cagaacaggc atttctggtg tccgggcttt tgtcacaggg
    45601 gtaaacagta acggtaagag tgccagtgca atagtgcttt gtttcacttt attttctcca
    45661 tgtacaaata cattaaaaaa taaaaacaaa gcgactataa gtctcggccg tgacaacttt
    45721 atgacagctg ttgaaaagat taacttttta ctgacgagga tgcttcaata acttctttac
    45781 ataattgcgc agcagctccg tatcgtcgtc aggaatgctg gcatcaggct ttacctcgta
    45841 aagagccccc tccacctgtt caatcaagcg ctgttggtca ttttgtgcca tattgcgaag
    45901 cattgcagtg acgataatct ccaaagcttc tacctgcgcg cacagttctt tcgactcttc
    45961 ttctttttgg gcaagcttta ataacaactc agcaatgaga tttttcatgt ctgtatttcc
    46021 ttatccaacg tatggagaag ttaacattat gtttcacaac ttcatagtgt ctggaagtat
    46081 tatttttatg acaaaggaaa tactttgcgc aaatgttctt tgtttgatta ccagcgaaac
    46141 gtttctcata tttaaataat taaccacaca aagaacaggc ctttaattat caacctttat
    46201 tataaacaaa agttatgaaa tatattctct gcgtttactt acaactaaga gaaaaaactc
    46261 accgcatggc catttaagcc aggaataaat tagcttcccg ttcatcattt ttgtgatctc
    46321 gcgcacatct ttgtaataac gcactttctt caccattacg ttgcacgctt tgtcagcaga
    46381 agattgcaac attctgtcag aaggccgcga atcccccgtt ttgtcgtggt aatgtatgca
    46441 ctttgtgtta gacgggatag gtttttaaga tggaaaaact gcgggtagga atcgtttttg
    46501 gtggtaaatc agcggaacat gaagtgtctc tgcaatcggc aaaaaacatt gtcgatgcca
    46561 ttgataaaag tcgcttcgac gttgtgctgc tgggcattga taaacaaggg caatggcacg
    46621 tcagcgatgc cagcaattat ctgctaaatg cagacgatcc tgcccatatt gcgttgcgcc
    46681 cttcggcgac tagccttgcg caggtgccaa gtaaacatga gcatcagctt atcgacgcgc
    46741 aaaacggtca gccgttaccg acggtggatg tcattttccc gattgtccac ggtacgctgg
    46801 gcgaagatgg ttccttgcag ggaatgctgc gggtcgccaa tttaccgttt gtaggttctg
    46861 atgttctggc ttcagcagcc tgtatggata aagatgttac caaacgtctg ctgcgcgatg
    46921 ccgggctgaa cattgcgcca tttattaccc tgacgcgcgc caatcgtcac aacattagtt
    46981 ttgccgaagt ggagtctaaa ctgaggttac cactgtttgt aaaaccggct aatcagggct
    47041 cttctgttgg tgtcagcaaa gtaaccagtg aagaacagta cgcaattgcc gtcgatctgg
    47101 cgttcgaatt cgatcataaa gtgatcgttg agcaagggat caaaggtcgt gagatcgaat
    47161 gcgcagttct gggcaacgac aacccgcaag ccagcacctg tggcgagatc gtactcacca
    47221 gcgacttcta tgcctacgac accaagtaca ttgacgaaga tggcgcgaaa gtggtagttc
    47281 cggcagccat tgcgccagaa atcaacgata agatccgggc gattgccgtt caggcttatc
    47341 aaacgttggg atgcgcaggc atggcgcgtg tagacgtgtt tttaacccca gagaacgaag
    47401 tggtgatcaa cgagatcaac acactgcctg gcttcaccaa catcagtatg tatccgaagc
    47461 tgtggcaagc cagcggtctg ggttacaccg atctgatcac acgtttgatt gagctggcgc
    47521 tggagcgtca cgctgcggat aacgcattga aaaccacaat gtaatatttt aacgcccgat
    47581 aatattctta ttgggcgttt tcagtttctt catcgcgacg acgaataatc aaccccgcca
    47641 gccagaaact aatcacccac gtcaccagac cgacggcata ggtctgccaa ccttttgctt
    47701 caaatcccag taaccccacc gcgccattaa gaatgaatat aaggccaatg gcgaacgcat
    47761 agtagtgcca gtcacggcgg atttttacag gcagattcat ggcagctccg ttaacgaaaa
    47821 acaacatcct tgcatattct gtaacaaccg tctgtggtgt agacaacatt ttcgtaattg
    47881 ttaataaatt taacttaagg ttgccatttt gtgactcaca acgaagattc acgcttcggg
    47941 aatattccca acatcaattt accatgattc tgatattgac aatctaaatg acctgtcaga
    48001 cttgccataa gccgctggta tcacaccaga agagaagcag atgacttccg gaggttttta
    48061 tggctgattt caccctgtca aaatcgctgt ttagcggaaa atatcgcaat gcctcttcaa
    48121 cgcctggcaa cattgcctat gcgttgtttg tgctgttttg cttttgggct ggggcgcaat
    48181 tgctgaacct gttagtgcat gcgcccggcg tctatgagcg tttaatgcag gtccaggaaa
    48241 caggtcgccc acgggtggaa attggtttag gtgtcggcac cattttcggg ctgatcccgt
    48301 ttttagtagg ctgcctcatt tttgcagtgg tggcgctatg gctgcactgg cgacatcgcc
    48361 gccagtaaga tttacgttgc cagatacggc ctaaaacgcc ttatctggca tgacattcct
    48421 gtttgatcac tgtttcatac aacgcgcccg ccgttcgtct acgcgcttcg caaaccaggc
    48481 cgtagtcagg ttgcgggtga ttttcgggct ttccagttga atgccaggca acatttcgcg
    48541 gggtagtgat ttacccgttt tcgcttcggc aagttgataa actttcttgt acagcgccgt
    48601 ctcctcaaaa gagaagctgt cacctttctc taactgacgg cgaatttcgc tgtcgctcat
    48661 ccccagtttt cccgccagtt tgcgcgttgc cagttcggtt ttcccgggtt ctttactgtc
    48721 gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg ctggccttac tgaccgcgtt
    48781 ctgaaacgcg gcattacggc tggcgtacca gccagcgtta aaatcagcaa agcggtatat
    48841 cggtgcgcta tagctggcgg gataattcag taaatggtaa gtaccaaacc acaacccgcc
    48901 gcggcgactg aagacttcct gacgaactgt accgtccatt ttccacggat accctttggt
    48961 atgctgttcg gcaaaagcaa tgctgacctg catcggccca ccggtgcgca ccggattaag
    49021 cgaaccaaac agcgtctgcc ccattggtac catgctgatc agatcgtcaa aaatcgcgct
    49081 caattgcttt tctgtacgca ccgaatctaa ccgttcgcta taacttttgc cgtttggcga
    49141 tttgattttt agcgcggtat gaaccagaaa agcaggaatg tgcatccgtt cagcacgacg
    49201 gtcaatctct tgccaggcga tcttgcttaa gcccggaacg gccggatctg cctgataatt
    49261 tgactcctgc tgcgccaccg ccagcaccga gcagacgttt tccagcgtcg gtgccagccc
    49321 ctgactttca aaggtggtgg cgagatcttt cgcccaggca tcgcggtctt tcacgctcgc
    49381 aggcatcttc tgccgcacga cgctcgctac atctatcgct ttttcgccct ttttcaacgg
    49441 ttgcggagcc tgactactac accccgctaa aaccagaacc gccaacaatg acagcgaaga
    49501 tagatgattg acgcgcgaca ttacttctcc tttttagctc aaggtatggg ttacttcctg
    49561 aattttgtca ccatccagct catgttcaaa gctgcgcaga cgtttgtaga tagacatcaa
    49621 ctcaaccagt gtggtccatg agttaatcag gtactggaaa gcaccgcgaa cctgaccaaa
    49681 aacgttggta atctgcgtca tcaggccgag cgtaatcgta ccggcaacaa ttgacggaaa
    49741 cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag agaatgcggg cgatgttgaa
    49801 atacatatag tgaaaataga ggcgaaaata gtttttccgt acggcgctaa acagctcgcg
    49861 taccgtaggc ggcgtcgcgc gcgtggcatc gtcttcacca taaaccagct ctttacggta
    49921 ggcggcctct actcgctggt ttttaaactc cagccccggc agtttgatcc ctaccactgc
    49981 cagcaatccg gtccccatca gcgaccagac gatagcggca atcaccagac catacggaat
    50041 gtgcccgata atcggcagct ccggcacatg cgcggagagc gttaccagca ccggcaggaa
    50101 ggcgaccaac gtcatgatgg cgttgataaa actgacgccc atattctcca gcgttgaagc
    50161 aaaacgcatg gtgtcttcct gcacacgctg tgcggcacct tcgatatgac gcagttgttg
    50221 ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag cggaacacat agtgactgac
    50281 aaagaagttg ttcagtacac tgatcaccac cgcgatcagc gcaatcccca gaaagacgcc
    50341 cacttcgcgg taaaattgtt cgatagtgac tttatgcggc gaacttagcg cggtttgaat
    50401 cagatcatag aacggtgcat accaggcgtt gacggcgact ccgacttcca ccaaaaacca
    50461 ggtgacgaag atgatcagtg cagtaccgag tattgaccag tattgccaac ggtgcgggct
    50521 gtagataaac cagaaaaatg caaaaagtcc tacgcaaaca atgtagtaag cgtaaaaaat
    50581 caggaaatcc aacgaccaga aacgcgcggc gctaatcggg atctgcccgg aagcgccggt
    50641 gatacgcgcc acccagtctc ccccaccggc ttgccagaag ataacggcaa tcaatgccca
    50701 aacaaaggcc gagagaaaaa acgctcccgg ctttgggaaa aaagacttaa acatatgcac
    50761 tcctgctaac ttcttatcgt tttaacaact gctgtgtatt aaccgcatga ccactacaaa
    50821 gcgtcagcca aatgaaaccg cccattgtga cccatagcaa ggcgcttagt tcgccagcct
    50881 gaccataaga attgtttcga cggatttcac aaaccaaaga ttgtttacat aagcggatta
    50941 acacgcgcag gatcacaaat gcaggcattg gtgagcagga aaaagatcgt gtttacgcgc
    51001 cgttacgttt ttaacactat tattgcacta attcgccctt tgcaatccat caatgagtag
    51061 tataaatacg ctcagttacc ttcattcaat ctatggacac caccgttgaa acgtagtctg
    51121 cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg tccatgcggc gcagccgatc
    51181 actgaaccgg agtttgcctc tgatattgtc gatcgttatg ccgatcatat tttttacggc
    51241 agcggtgcca cggggatggc gctggttgtt atcgacggta accagcgcgt ctttcgcagt
    51301 tatggcgaaa cgcgacctgg taataacgtt cgcccgcagc tggattcggt cgtgcgtatt
    51361 gcttccctca ccaagctaat gaccagtgaa atgctggtga aattgctcga ccaggggacc
    51421 gtgaagttaa acgatccgct aagtaaatat gctccgccag gcgcacgcgt gccaacctac
    51481 aatgggacgc ccattaccct agtgaatctg gcaacccata ccagcgccct gccccgtgaa
    51541 cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc caacgcgcga gcaacgctgg
    51601 aaataccttt ctacggcgaa gctgaaagcc gcgccaggca gtcaggcggc gtactctaac
    51661 cttgcgttcg acctgctggc agatgcactg gcgaatgcgt ccggcaagcc ttatacccag
    51721 ttgtttgaag agcagattac ccgtccattg gggatgaaag acaccaccta caccccctca
    51781 ccggatcagt gccgccgttt aatggttgct gagcgcggtg ccagtccgtg caataacacg
    51841 ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg gcgatatgat gcgctggatg
    51901 cagcagtatt tatcatctga tttttatcag cgcagtaacc aggcagatcg catgcagacg
    51961 ttgatttatc agcgcgcgca gtttaccaaa gtgattggca tggatgtccc cggcaaagcg
    52021 gatgcgcttg gtttaggctg ggtatatatg gccccgaaag agggtcgtcc ggggattatt
    52081 cagaagacag gcggtggtgg cggtttcatt acctatatgg cgatgatccc gcagaagaat
    52141 attggcgcgt ttgtggtggt cactcgttcg ccgttgacgc gctttaaaaa tatgagcgat
    52201 ggcatcaatg atctggtgac cgagctaagc gggaataaac cgttggttat ccctgcatcc
    52261 tgattaatac tctgaaggca aacggttgat agcgaccagt ttgcctttat tcatttcgat
    52321 atagccgcct ttacgcaaag cagcgagaac ttctgcgacg acagaacgcg atatgcgtgt
    52381 acgttgatgg atataattca tcacaccaat acgtgatcgc aattcttcat tccagtcaat
    52441 cattgataat aatgttgcgc ggatttgttc gtaggaatta tgcccaatga gctgcacgtc
    52501 gcgtaattcc agaattctat tttgccaggc taaccagtaa aaagcgtctc gccagagttg
    52561 attttgttca ataagcgtaa tggtttgttt ggctggtaga tgatatcccg tacaatttcc
    52621 ttctgatatt aatttgtatg gtatatcgtt tttcattaaa ccatcagcca gccccataat
    52681 ataagaagcc tgggtaatac cgataagtac gttttcttct ctacgcagag agataacgcc
    52741 ctctaaaata aagaaagtat cttcgctatt gacatcactg gaaaatataa cttgcttttc
    52801 attattaaac tcgaagcgcg taccgtatct ggacaaacat ttatcgagct taccaaattc
    52861 ctgaagaggt ttaactacag ataacatttg cgcgtccttt gcagtaatgc ccgtcaaatc
    52921 cttgacgggc attatttaga ttaaattacc agaatttctt cggagtgaag aatattacca
    52981 agtatattta acacccacgt tcgcggacca gtcttgatct acgtcaccac caccgaggta
    53041 gttagcatcg gtataggcgc tgaagttctt ggtgaagctg aactgagtac ccagaccaac
    53101 acgtaccgca gacccttcag taccgttatc gatggaatca ccgttcacat cgttatcgtt
    53161 gtttgagtcg tcgtagacgt aagccagttt gaagtacgga gtcagagcct gatcttcgct
    53221 gtaggtgaag gtataacctg catctacacc cagttcataa cgcatgctgt cgtaagactg
    53281 accgtcaact ttcatgtcgt tgctcagctg gtagtcatca ccagactgga acagaccaga
    53341 aacgctgccg taaggagtct cgtaaccagc atcacccagt ttgaagtcgt aaccggcttt
    53401 caaaccgaag ccccacgcgt cagagttggt gctaccgtca acgtaagtac cgttgctcat
    53461 ggttgcagac aggtcgttgt tgaagtgaga gtagctcaag ctaccatcaa caaagacgtt
    53521 gttcgcgaag tgagcagaag agtagatgta ggcagtctgg ctgtcttgat ccacctgacc
    53581 agaacggtca ttcatgtcac ctttagcgaa gcctgcagcc gcaccgacga tccacttagc
    53641 gttgttaccg tcaattttgg tatcaacacc gaccatgatg ccgttaacat cctgatcata
    53701 gttgatggtg ccgttgtcgc cgttgaagtt accaccgaag tagcttaccc atgcgccgcc
    53761 gttatcagcc aggccatgac gagagttggt cagacgagta ccaacggtgt cttgttccag
    53821 gttccagata ttggtgttcg cagacgggat gctcagcgcc atgttagcgt agtcggtcag
    53881 ctccatctgt tgcagaacaa cggtgttacc gcgctgttca gcctgatagg tgtatgcacc
    53941 caggtcagct ttgttagcag cagagaaggt cgcgttggtg ttagcgtcgt ttacccgaat
    54001 aagttcttta tctttgtaat cagcgatagt gccagcgcca gttgcgttgt cgatacgaac
    54061 tttgtagtta ccggttgcag caacaacgtt atcaacagag ctgttatcca gttcagtacc
    54121 gttgtctacg tcgccgttac cattgatagt caggtgacca tcagagttca tagcaacaat
    54181 accgtaaccg tagttagact tggaagtatc ccacgtacgg tcgttcacca gatcagcgtt
    54241 cagaacgtaa tcgctgctgt tgatgtcgaa cacaccagaa attacgttgc cattatggtc
    54301 agcaatgttg ccgctggtca gttccagttt atcagtacgg aaagcttcag tatcgaactg
    54361 atcaacattc acatccagca caccaccgtt agtcacagtg atagtattgg tatacagatt
    54421 agaataatta ttccaaccgg tagattcggt taaattaacg gtactgtagc tatcaacagt
    54481 caggtggtct gtagcaacca cgccatttgc accaatattc agtgaagaac cgttggttaa
    54541 accaatggta tctgatacca gagaggaatc agtaacattt acctgagagc cattattta
//
LOCUS       DAJTOR010000006        47212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTOR010000006 DAJTOR010000000
VERSION     DAJTOR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.006"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            17..754
                     /locus_tag="OO249_000355"
     CDS             17..754
                     /locus_tag="OO249_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5634774.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGKASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            865..1875
                     /gene="pyrD"
                     /locus_tag="OO249_000356"
     CDS             865..1875
                     /gene="pyrD"
                     /locus_tag="OO249_000356"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5634775.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            2049..2591
                     /gene="zapC"
                     /locus_tag="OO249_000357"
     CDS             2049..2591
                     /gene="zapC"
                     /locus_tag="OO249_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5634776.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNVLVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(2588..3697)
                     /gene="ycbX"
                     /locus_tag="OO249_000358"
     CDS             complement(2588..3697)
                     /gene="ycbX"
                     /locus_tag="OO249_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5634777.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            3941..6049
                     /gene="rlmKL"
                     /locus_tag="OO249_000359"
     CDS             3941..6049
                     /gene="rlmKL"
                     /locus_tag="OO249_000359"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5634778.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGALSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            6061..7968
                     /locus_tag="OO249_000360"
     CDS             6061..7968
                     /locus_tag="OO249_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5634779.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNIAEGKQEVMVNGKPRHVLSYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            8098..9351
                     /gene="pqiA"
                     /locus_tag="OO249_000361"
     CDS             8098..9351
                     /gene="pqiA"
                     /locus_tag="OO249_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5634780.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            9356..10996
                     /gene="pqiB"
                     /locus_tag="OO249_000362"
     CDS             9356..10996
                     /gene="pqiB"
                     /locus_tag="OO249_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5634781.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            10993..11556
                     /gene="pqiC"
                     /locus_tag="OO249_000363"
     CDS             10993..11556
                     /gene="pqiC"
                     /locus_tag="OO249_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5634782.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            11812..11979
                     /gene="rmf"
                     /locus_tag="OO249_000364"
     CDS             11812..11979
                     /gene="rmf"
                     /locus_tag="OO249_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5634783.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(12049..12567)
                     /gene="fabA"
                     /locus_tag="OO249_000365"
     CDS             complement(12049..12567)
                     /gene="fabA"
                     /locus_tag="OO249_000365"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5634784.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(12636..14396)
                     /locus_tag="OO249_000366"
     CDS             complement(12636..14396)
                     /locus_tag="OO249_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5634785.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPNLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14582..15034
                     /gene="matP"
                     /locus_tag="OO249_000367"
     CDS             14582..15034
                     /gene="matP"
                     /locus_tag="OO249_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5634786.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(15110..16150)
                     /gene="ompA"
                     /locus_tag="OO249_000368"
     CDS             complement(15110..16150)
                     /gene="ompA"
                     /locus_tag="OO249_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5634787.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(16507..17016)
                     /gene="sulA"
                     /locus_tag="OO249_000369"
     CDS             complement(16507..17016)
                     /gene="sulA"
                     /locus_tag="OO249_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5634788.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            17235..17863
                     /gene="sxy"
                     /locus_tag="OO249_000370"
                     /pseudo
     CDS             17235..17863
                     /gene="sxy"
                     /locus_tag="OO249_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
     gene            complement(17826..19988)
                     /gene="yccS"
                     /locus_tag="OO249_000371"
     CDS             complement(17826..19988)
                     /gene="yccS"
                     /locus_tag="OO249_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5634789.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(19998..20444)
                     /locus_tag="OO249_000372"
     CDS             complement(19998..20444)
                     /locus_tag="OO249_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5634790.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            20567..22627
                     /gene="helD"
                     /locus_tag="OO249_000373"
     CDS             20567..22627
                     /gene="helD"
                     /locus_tag="OO249_000373"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5634791.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPLPVNRLEEFDNCREAWRK
                     CQAWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLA
                     GAGSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITART
                     FHALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEE
                     MQWSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIK
                     LMAPLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAA
                     LLAALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNS
                     RIGKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERIL
                     ILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAAR
                     ESIMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPV
                     ARKP"
     gene            complement(22659..23117)
                     /gene="mgsA"
                     /locus_tag="OO249_000374"
     CDS             complement(22659..23117)
                     /gene="mgsA"
                     /locus_tag="OO249_000374"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5634792.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23213..23875)
                     /locus_tag="OO249_000375"
     CDS             complement(23213..23875)
                     /locus_tag="OO249_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5634793.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            24048..24461
                     /locus_tag="OO249_000376"
     CDS             24048..24461
                     /locus_tag="OO249_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5634794.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(24506..24823)
                     /gene="hspQ"
                     /locus_tag="OO249_000377"
     CDS             complement(24506..24823)
                     /gene="hspQ"
                     /locus_tag="OO249_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5634795.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(24881..26071)
                     /gene="rlmI"
                     /locus_tag="OO249_000378"
     CDS             complement(24881..26071)
                     /gene="rlmI"
                     /locus_tag="OO249_000378"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5634796.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            26166..26444
                     /gene="yccX"
                     /locus_tag="OO249_000379"
     CDS             26166..26444
                     /gene="yccX"
                     /locus_tag="OO249_000379"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5634797.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(26441..26770)
                     /gene="tusE"
                     /locus_tag="OO249_000380"
     CDS             complement(26441..26770)
                     /gene="tusE"
                     /locus_tag="OO249_000380"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5634798.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(26861..27520)
                     /gene="yccA"
                     /locus_tag="OO249_000381"
     CDS             complement(26861..27520)
                     /gene="yccA"
                     /locus_tag="OO249_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5634799.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(27727..27814)
                     /locus_tag="OO249_000382"
     tRNA            complement(27727..27814)
                     /locus_tag="OO249_000382"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27778..27780),aa:Ser,seq:tga)
     gene            28241..29359
                     /gene="hyaA"
                     /locus_tag="OO249_000383"
     CDS             28241..29359
                     /gene="hyaA"
                     /locus_tag="OO249_000383"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5634800.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            29356..31149
                     /gene="hyaB"
                     /locus_tag="OO249_000384"
     CDS             29356..31149
                     /gene="hyaB"
                     /locus_tag="OO249_000384"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5634801.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            31168..31875
                     /gene="hyaC"
                     /locus_tag="OO249_000385"
     CDS             31168..31875
                     /gene="hyaC"
                     /locus_tag="OO249_000385"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5634802.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDGTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            31872..32459
                     /locus_tag="OO249_000386"
     CDS             31872..32459
                     /locus_tag="OO249_000386"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5634803.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            32456..32854
                     /gene="hyaE"
                     /locus_tag="OO249_000387"
     CDS             32456..32854
                     /gene="hyaE"
                     /locus_tag="OO249_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5634804.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            32851..33708
                     /locus_tag="OO249_000388"
     CDS             32851..33708
                     /locus_tag="OO249_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5634805.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            33842..35386
                     /gene="appC"
                     /locus_tag="OO249_000389"
     CDS             33842..35386
                     /gene="appC"
                     /locus_tag="OO249_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5634806.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            35398..36534
                     /gene="appB"
                     /locus_tag="OO249_000390"
     CDS             35398..36534
                     /gene="appB"
                     /locus_tag="OO249_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5634807.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADVRWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLSSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            36547..36639
                     /gene="cbdX"
                     /locus_tag="OO249_000391"
     CDS             36547..36639
                     /gene="cbdX"
                     /locus_tag="OO249_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5634808.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            36719..38017
                     /gene="appA"
                     /locus_tag="OO249_000392"
     CDS             36719..38017
                     /gene="appA"
                     /locus_tag="OO249_000392"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5634809.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNV
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(38132..40312)
                     /gene="etk"
                     /locus_tag="OO249_000393"
     CDS             complement(38132..40312)
                     /gene="etk"
                     /locus_tag="OO249_000393"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR5634810.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLHDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(40332..40778)
                     /gene="etp"
                     /locus_tag="OO249_000394"
                     /gene_synonym="yccY"
     CDS             complement(40332..40778)
                     /gene="etp"
                     /locus_tag="OO249_000394"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5634811.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(40766..41905)
                     /locus_tag="OO249_000395"
     CDS             complement(40766..41905)
                     /locus_tag="OO249_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5634812.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTRNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(41951..44047)
                     /locus_tag="OO249_000396"
     CDS             complement(41951..44047)
                     /locus_tag="OO249_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5634813.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVNDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(44047..44793)
                     /locus_tag="OO249_000397"
     CDS             complement(44047..44793)
                     /locus_tag="OO249_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5634814.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(44790..45434)
                     /gene="gfcB"
                     /locus_tag="OO249_000398"
     CDS             complement(44790..45434)
                     /gene="gfcB"
                     /locus_tag="OO249_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCR5634815.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(45541..45846)
                     /locus_tag="OO249_000399"
     CDS             complement(45541..45846)
                     /locus_tag="OO249_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HCR5634816.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(46288..46500)
                     /gene="cspH"
                     /locus_tag="OO249_000400"
     CDS             complement(46288..46500)
                     /gene="cspH"
                     /locus_tag="OO249_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5634817.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            46786..46998
                     /gene="cspG"
                     /locus_tag="OO249_000401"
     CDS             46786..46998
                     /gene="cspG"
                     /locus_tag="OO249_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5634818.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            47009..47197
                     /locus_tag="OO249_000402"
     CDS             47009..47197
                     /locus_tag="OO249_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5634819.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVTLQIRTDHAPLDFTK"
BASE COUNT        11627 a        11727 c        12404 g        11454 t
ORIGIN      
        1 cagtcgggta aagtgaatgc cagcgcgaca tttgtacttc attatgacta atacgtggaa
       61 cagattggcg ctcttgattt tcgccgtttt atcgctgctg gtggcgggtg aattgcgggc
      121 aggagtggtg gtcggcggaa cgcgatttat ctttccggca gacagagaat cgatatctat
      181 tttactgact aataccagtc aggaatcctg gctgattaac agtaaaatca accgcccaac
      241 gcgttgggcc ggggggaaag cgtcgacagt gccagcacca ttactggccg ctccgccact
      301 tattctcctg aagcccggta ctacaggcac gttgcgcttg ctgagaacgg aaagcgacat
      361 cttgcctgtg gatcgcgaaa cgctatttga gttaagcatt gccagcgtgc catccggcaa
      421 agttgaaaat cagagcgtaa aagtggcgat gcgctcggta tttaaactgt tctggcgacc
      481 cgaaggtttg ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga cacggaattc
      541 gcagggtgta caactcacta acccaacgcc ttactacatt aacctgattc aggtgagcgt
      601 aaatggtaaa gcgttaagta atgcgggagt tgtgccgcct aaaagccagc gtcagacaag
      661 ctggtgtcag gcaatcgcac cgtgtcatgt cgcctggcgg gcgataaatg attacgacgg
      721 gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac gtttgcgttt
      781 tttttgccgc aggtcaattc ccttttggtc cgaactcgca cataatacgc ccccggtttg
      841 cacaccggga atccaggaga gttcatgtac taccccttcg ttcgtaaagc ccttttccag
      901 ctcgatccag agcgcgctca tgagtttact tttcagcaat tacgccgtat tacaggaacg
      961 ccgtttgaag cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg catgggcctg
     1021 acgtttaaaa atccgcttgg tctggcagcc ggtcttgata aagacgggga gtgcattgac
     1081 gcgttaggcg cgatgggatt tggatcgatc gagatcggta ccgtcacgcc acgtccacag
     1141 ccaggtaatg acaagccgcg tctctttcgt ctggtagatg ccgaaggttt gatcaaccgt
     1201 atgggcttta ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa ggcccattat
     1261 gatggcgtcc tgggtattaa catcggcaaa aataaagata cgccagtgga gcagggcaaa
     1321 gatgactatc tgatttgtat ggaaaaaatc tatgcctatg cgggatatat cgccatcaat
     1381 atttcatcgc cgaatacccc aggattacgc acgctgcaat atggtgaagc gctggatgat
     1441 ctcttaaccg cgattaaaaa taagcaaaat gatttgcaag cgatgcacca taaatatgtg
     1501 ccgatcgcag tgaagatcgc gccggatctt tctgaagaag aattgatcca ggttgccgat
     1561 agtttagttc gccataatat tgatggcgtt attgcaacca ataccacact cgatcgttct
     1621 cttgttcagg gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg tccgcttcag
     1681 ttaaaaagca ccgaaattat tcgccgcttg tcactggaat taaacggtcg cttaccgatc
     1741 atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc tgcgggtgcc
     1801 tcactggtgc aaatttattc tggttttatt tttaaaggtc cgccgctgat taaagaaatc
     1861 gttacccata tctaattatt tcttcgaatt cctctttata agataatcag ggctttattt
     1921 tcagccctgg ttgttttata ttcatcgctg ttgcttattt agacattttg tacttttatt
     1981 gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct gcggttttat agggtagggg
     2041 agaggcagat gcgaattaaa ccagacgata actggcgttg gtattacgat gaagagcacg
     2101 atcgaatgat gctcgattta gccaatggta tgctatttcg ctcacgtttt gcgcgcaaaa
     2161 tgttgactcc agatgccttt tctcccgcag gcttttgcgt tgacgacgcc gcgctctatt
     2221 tctcttttga agaaaagtgc cgcgatttta atttatctaa agaacaaaaa gccgaactgg
     2281 tgctgaatgt actggtagca attcgctatc tcaaaccgca aatgccgaaa agctggcatt
     2341 ttgtttccca tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc gtttggttga
     2401 gcgatactca cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat gccgcactat
     2461 gcctgctggc acaaccctgc gttgttattg cgggtcgcgc gatgcagttg ggcgacgcca
     2521 ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc agcctcgaac
     2581 aggcagtcta acgcgccaac ttaagtgcag tcttcggtac acagctacag caaagaatgg
     2641 tgccatcatc gcccattgct gattttttca gcggcgagac ttcgccttct aaaatctgaa
     2701 cacggcaact tccacaaatg cccgcgcggc aagaataagg gatacgaatt ccctgatttt
     2761 ctaattgctc cagcaacacc tgttggttat ttccacgaaa tgcctgtccc tgccagtcaa
     2821 tatctacatt tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg gcagctgcgc
     2881 cgtaaatttt tgccggagcc gttgccagaa tttccacctc atcgccaacg cgaatcacgc
     2941 cgctattacg ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc tgggcagtgc
     3001 ggaaagattg caatgttttt aatggttcgc ctgccggatg tttttgccct ttttctgggc
     3061 tgacggtggt gaaaatacag cggctacaag gtttaaccac atcaaacacc acatcaccaa
     3121 tgcgaatcac tttccagcga tcttcttccc aggctgacgc gccggaaacc accagattgg
     3181 ggcggaattg ctccattttt acactggccg gacaacgttg ttggagatca cgtaacgagg
     3241 cttcgttagc aagaaggtaa ggatagccat cagcaaatga cagaggtaca gtgttgtggc
     3301 gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg cgggagaaaa
     3361 atccacttag ccatttgttg atcgcgtctg gcgcaattcg cgcggtaaaa tgtgtgcccc
     3421 aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat gcgctactgc
     3481 catctggtgc ggtgagatgc aagccatcat gcacaggtga aggagtaaac cgtaccatct
     3541 ggggaaactg gcgagcggta ataaacgtac cgtcaggttc cgtgatcata aagatgcgat
     3601 cgaaggccag accactgaca tcagccagag catgtgtaag accaatgccg cgcatcgatt
     3661 taacaggatg aataaaaagc cgggttaatg tcgccacggt gcggtcctca aatgaaaata
     3721 agccctcaac tttatgacat gacgcgctta ttagctataa tgcgcaacaa ttttcttagc
     3781 gcctgtccca ttaggctatt ttatttgtca gtttggccct gggttgtgct cgaaatcctc
     3841 acgtacttgt gtacgctccg ttttctccgc gctgtccatg tccagactga ctgcaacaat
     3901 tacgcctact gcggtaggct cttagagtaa aagtgacgat atgaattctc tgtttgccag
     3961 tacggcccgt gggctggaag agctgttaaa aactgaactg gaaaacctgg gggccgttga
     4021 atgccaggtg gttcagggtg gggtccattt caagggcgac acacggcttg tttaccagag
     4081 cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt gtaaggttta
     4141 cagcgattta gacctctatc tcggtgttca ggcgatcaac tggacagaga tgtttaatcc
     4201 tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac accatccgca acagtcagta
     4261 cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa atctgccgcg
     4321 tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac gtctggctgc ataaagaaac
     4381 cgccagtatc gctcttgatc tgagtggtga tggtttacat ctgcgtggct atcgcgatcg
     4441 tgctggtatt gcgccgatca aagaaaccct ggcagccgct attgtgatgc gatccggctg
     4501 gcagcctgga acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc tgattgaagc
     4561 ggcgatgctg gcgaccgatc gcgcacccgg cttgcaccgt gggcgttggg gctttagcgg
     4621 ctgggcgcag catgatgaag ctatctggca ggaagtgaaa gcggaagcgc aaactcgcgc
     4681 ccgtaaaggc ctggctgagt atagctctca tttttacggt tcggacagcg acgcacgggt
     4741 gattcaacgt gcacgcacta acgcccgtct tgcggggatt ggtgaactga tcacctttga
     4801 ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg gcacagtgtt
     4861 gagcaacccg ccatacgggg aacgtctgga cagcgaaccg gcgctgattg cgctgcatag
     4921 cctgctgggt cggatcatga aaaaccagtt tggtggctgg aatctctctt tgtttagtgc
     4981 ctcgccggat ctgctaagct gcttgcagct gcgtgcagac aaacagtaca aggcgaaaaa
     5041 cggcccgctg gactgcgtac agaaaaatta ccacgttgcc gaaagcactc cagacagcaa
     5101 accggcgatg gtagcggaag actacgccaa ccgcctgcgt aagaacctca aaaaattcga
     5161 gaagtgggct cgccaggaag gaattgaatg ttaccgcctg tatgacgccg atctgccgga
     5221 atataacgtt gccgttgacc gttattccga ctgggtggtg gtgcaggagt atgcaccgcc
     5281 aaaaactatt gatgctcaca aagcgcgtca gcgtctgttc gatattatcg ctgcaaccat
     5341 ttcggtactg gggattgcgc caaacaaact ggtgctgaaa acccgtgaac gccagaaggg
     5401 caaaaaccag taccagaaac tgggcgagaa gggcgagttt cttgaagtta ccgaatataa
     5461 cgctcacttg tgggtgaacc tgacggatta tctcgatact ggtctgttcc tcgatcaccg
     5521 tatcgcccgc cgtatgctcg gtcagatgag caaaggcaaa gatttcctca acctgttctc
     5581 ttataccggt agcgccaccg tgcatgcggg attaggcggc gcgctcagca ccaccaccgt
     5641 ggatatgtcg cgtacttatc tggagtgggc agaacgcaac ctgcgtctga atggcctaac
     5701 cgggcgtgcg catcgcctga ttcaggccga ttgcctggcg tggctgcgtg aggcaaatga
     5761 acagtttgat ctgatcttta tcgatccgcc aaccttctct aactcaaaac gaatggaaga
     5821 tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa gatttgaaac gtctgctgcg
     5881 tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg atctcgacgg
     5941 cctggcaaaa ctgggactga aagcacaaga aattacgcaa aaaacgctct cccaggattt
     6001 cgcccgtaac cgccagatcc acaactgctg gctgattacc gcagcctgaa aggaatagta
     6061 atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc gcttctcgat
     6121 aacgcagaac tgcatatcga agataacgaa cgtgtttgtc tggtgggccg caacggcgcg
     6181 ggcaaatcga cgttaatgaa aatcctcaac cgtgaacaag ggctggatga cggtcgcatt
     6241 atttacgagc aagatttgat tgtagctcgt ctgcaacagg atccgccgcg taacgttgag
     6301 ggtagcgttt atgatttcgt tgccgaaggc attgaagaac aagcggaata tctgaaacgc
     6361 tatcacgata tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct caacgaactg
     6421 gcgaaggttc aggaacagtt ggatcaccac aacctgtggc agctggaaaa ccgcatcaac
     6481 gaagtgctgg cgcaactggg gttagatcct aatgttgcgc tgtcgtcgct ttccggcggc
     6541 tggttgcgta aagcggcatt aggacgcgcg ctggtgagta atccgcgcgt gctgttgctt
     6601 gatgaaccga caaaccacct ggatattgaa accatcgact ggctggaagg gttccttaaa
     6661 accttcaatg ggacgattat tttcatctcc cacgaccgtt cgtttatccg caatatggcg
     6721 acgcgcattg ttgatctcga tcgcggcaag ctggtgacct atccagggaa ttacgaccag
     6781 tacctgcttg aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc cgagttcgat
     6841 cgcaaactgg cgcaggaaga ggtgtggatc cgccagggga tcaaagcacg ccgtacccgt
     6901 aatgaaggcc gcgtacgcgc cctgaaagca atgcgtcgcg agcgtggtga acgtcgcgaa
     6961 gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa gatcgttttc
     7021 gaaatggaag acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga tttttcggcc
     7081 caggttctac gtggcgacaa aattgtcctg attggtccga atgggtgcgg caaaaccacg
     7141 ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca cgttggcacc
     7201 aaactggaag tggcttattt cgatcagcac cgcgcggaac tggatcccga taaaacggtg
     7261 atggataaca ttgccgaagg taagcaagag gtgatggtta acggcaagcc acgccacgta
     7321 ttgagctatt tgcaggactt tctgttccat ccgaaacggg cgatgacgcc ggtacgtgcg
     7381 ctttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa accaagcaac
     7441 ttattgattc ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga actgctggaa
     7501 gaactgatcg acagctatca gggcacggta ttgctggtta gccacgatcg tcagtttgtc
     7561 gataacaccg ttacagaatg ttggatcttc gaaggcggcg gtaaaattgg tcgttatgtc
     7621 ggcggttatc atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa acagcctgcg
     7681 gtgaaaaaaa ccgaagaagc cgccgcggca aaagcagaaa ctgtaaaacg cagcagtagc
     7741 aaactaagct ataaattgca gcgcgaactg gagcagctac cgcaattgct cgaagatctg
     7801 gaggcgaagc tggaagccct acagacgcag gtggcggatg cttccttctt cagtcagcca
     7861 catgagcaga cacaaaaagt gctcgccgat atggctgctg cagagcagga gctggagcaa
     7921 gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc gcaaaatagt
     7981 cgatttaaac agagacagct gacaacggta aaattgcctg atgaactggg tttatcaggc
     8041 atgaaaacca acactttgtt agtcatctgt aaaatagcgc atcattaagg agtaccaatg
     8101 tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat gctggtggcg
     8161 ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac aacgttaacc
     8221 gtggcgtggg atgcccctcg gcagcgtccg accgcctatg cgttggctgc actgttcatg
     8281 ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt taccagtgaa
     8341 attacattac tggaaattcc cggcgtgctt ttttctgagg actacgccag cctcggcacc
     8401 tttttcctgt tgtttgtgca actggttccc gcgttttgtc tgataaccat tctgttactg
     8461 gtgaatcgcg cggaattacc ggtccgttta aaagagcaac tggcacgggt gctttttcaa
     8521 ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg tgctggtcag tttcgttaaa
     8581 ctgatggctt acggcagcat tggggtaggc agcagctttc tcccctggtg tttattttgt
     8641 gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga cgacatcgcc
     8701 ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca cggggatacg tcaggggctg
     8761 cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg cccgcgttgt
     8821 agtaccaaag ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc gctgcttgta
     8881 acgtccatca tgctgtatct tccggctaat attttgccca tcatggtgac ggatttatta
     8941 ggctcgaaga tgccgtcgac gattctcgct ggggtcattc tgttatggag cgaaggctct
     9001 tatcccgtcg ctgcggtgat ctttctggcc agtattatgg tgccaacgtt aaagatgatc
     9061 gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca agcgcgacag tgaaagaatg
     9121 catttgattt atgaagttgt tgagtttgta ggccgctggt cgatgattga cgttttcgtt
     9181 atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta tccggcaatg
     9241 ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt tttctgctat gacgtttgac
     9301 ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg acagtatgga
     9361 atctaataat ggggaagcca aaatccagaa agtgaagaac tggtctcccg tgtggatatt
     9421 tcctatcgtc acggcgctca ttggggcctg ggttctattt tatcattaca gccatcaggg
     9481 accggaagtg accctgatca ccgcgaatgc ggaaggaatt gaaggtggca aaaccaccat
     9541 taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc acactggctg atgatttgac
     9601 gcacgttgaa atcaaagcgc ggctgaattc cggtatggaa aaattgctgc ataaagacac
     9661 cgtcttttgg gtggtgaaac cgcagattgg tcgcgaaggg attagcggcc tgggaacgct
     9721 gctgtctgga gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa tggataaata
     9781 cgatttgctg gactcgccac cgttggcccc gcctgatgcg aaaggtatcc gtgtgattct
     9841 cgatagcaaa aaagccgggc agctctcgcc aggagatccg gtgctgttcc gtggctatcg
     9901 ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca gttatcaact
     9961 gttcatcaat gcaccttatg accgactggt gaccagcaat gttcgcttct ggaaagatag
    10021 tggcattgcg gttgatctga cgtcagcggg gatgcgtgtg gagatgggct cattgacaac
    10081 gctgcttagt ggcggtgtta gctttgatgt gccggaaggt ctggatttag ggcagccagt
    10141 ggcaccgaaa acagctttcg ttttgtatga tgatcagaag agcattcagg attcgttgta
    10201 caccgatcac attgattatc tgatgttctt taaagattcg gtacgcggtc tgcaaccggg
    10261 agctccggta gaattccggg gtattcgcct gggtaccgta agcaaagtgc cattctttgc
    10321 gccgaatatg cgtcagacat ttaacgatga ttaccgtatt ccggtactga ttcgtatcga
    10381 gccagagcgg ctgaaaatgc agcttggcga aaatacggat gttgttgagc accttggcga
    10441 attgttgaaa cgtggtttac gcggatcgct gaaaaccgga aacctggtca ctggtgcact
    10501 gtatgttgat ctcgatttct atccaaatac gcctgcaata accggtattc gtgaatttaa
    10561 tggttatcag attatcccga ctgttagcgg cggcctggcg caaatccagc aacgactgat
    10621 ggaagcgttg gataagatca acaaactgcc attgaatccg atgattgaac aggcaaccag
    10681 tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg atagcatgaa
    10741 caagatcctc gctagccagt cgatgcagca gttgccgacg gatatgcagt caacgttgcg
    10801 tgaattgaat cgcagcatgc agggcttcca gcctggctcc gcagcctaca acaagatggt
    10861 ggcggatatg cagcgccttg atcaggtgtt gcgagaactg caaccggtgc tgaaaacgct
    10921 caatgagaag agtaacgcgc tggtatttga agcgaaggac aaaaaagatc cagagccgaa
    10981 gagggcgaaa caatgaaaaa gtggctagtg acgattgcag cactgtggct ggccggatgc
    11041 agctccggcg aaattaataa aaactattac cagttacctg tggtgcagag cggtacacaa
    11101 agtaccgcca gccagggcaa tcgtctgtta tgggtagagc aggtcactgt tcctgactat
    11161 ctggcgggga atggtgtggt ttatcaaacc agtgatgtga agtatgtgat tgccaacaac
    11221 aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc caacctgagt
    11281 acgcaactgc ccggctgggt ggttgcctcc cagcctctgg gaagcgtcca ggacacgctc
    11341 aatgttaccg taacggagtt taacggtcgc tatgatggca aggtcattgt cagtggtgag
    11401 tggctgttga accaccaggg acaactgatc aaacgtccgt tccgtctgga aggagtgcaa
    11461 actcaggatg gttacgatga gatggttaaa gtgctggccg gtgtctggag tcaggaagcc
    11521 gcttctattg cacaagagat aaagcgtcta ccttaattat aaagatttgt aagtataacc
    11581 gtctccggta tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa aatttgttcc
    11641 tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc acaaatatga cagtggcgtg
    11701 aattttgcgc attgacggca gttatgattc gcggtattgc ttaactgtga ttgcacattt
    11761 agtaatcact gttttctttt ccaccagaaa ccagtatgag ggaaacgagg catgaagaga
    11821 caaaaacgag atcgcctgga acgggcacat caacgtggtt atcaggccgg catcgccgga
    11881 cgctcaaaag aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg gctgggaggc
    11941 tggcgagaag ccatggcgga cagggtagta atggcctgat tctgtctctt taaaaagaaa
    12001 cctccgcatt gcggaggttt cgccttttga tactctgtct gatgataatc agaaggcaga
    12061 cgtatcctgg aacagaccga ctttcaggtc gctggcggta tagatcagac gaccatcaac
    12121 cagcacttcg ccatctgcca gacccataat cagacgacga ttaacaatgc gtttaaagtg
    12181 aatacggtag gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt tcacttcgcc
    12241 aacgcccagc gcgcggcctt taccttcgcc gcccagccag ccgaggtaga accctactag
    12301 ctgccacatt gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa tgaagtggca
    12361 tccgaagaac cacagatccg gattgatatc cagttctgct tcaacatacc ctttgtcgaa
    12421 gttaccaccc gtttcggtca ttttgaccac acggtccatc atcagcatgt tcggtgctgg
    12481 caattgcggg cctttagcgc caaacagttc accgcgacca gaggcaagaa ggtcttcttt
    12541 tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc cttattttat tgaagcacgc
    12601 aggatagcta acacgtgtac gctgaacaag tccgatcagt tcggaataaa ccagttcagc
    12661 caacgtaatg gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg cgcgatacgt
    12721 tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat cccacaccag atttaataat
    12781 aacggcagtg cgtcagtcac atcgtctact gcccagatgg tgaatttgcc ttcttctacc
    12841 gctttcacca gttcactgtg aagacttaaa tggcgaacgt tagctgtggg gataatgaca
    12901 ccttgtttcc cggttaactc acgttgctgg caaatagcaa agaagccttc gattttctca
    12961 tttaaaccac cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt gatagcgata
    13021 ctctgattca ccggcacatc ggcgagggcg cttatcaggg cgcagagttc agccatcgag
    13081 gcactatctc catcaacttc actgtatgac tgctcaaatg tcagcgatgc tgagaagggg
    13141 atctgttgct caagctgtag ttccgacatc aggaacgctt gcatgatcat catccctttc
    13201 gcatggatat tgccgccaag ttccgctttg cgttcgatgt cggtgaattc accatcgcca
    13261 atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg acccggaaat
    13321 tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc aatcaggatt
    13381 tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg ccattcacgc
    13441 tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca cagggaggcg
    13501 acctctttac actggcggag gatccactgc gggctaagcg gaagtgtttc ttgttcaccg
    13561 gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg ggcaggtaag
    13621 tgattatgtc tggcggtaaa tgtcacccag cgacaccact ggcttactga ctccgcatcg
    13681 acaatctgca gagtatcttc aaattcgcta taaatagcct gctctgaaag ctctggctcc
    13741 atctcctgga aatcagccaa tgattcgcgt tcgcctacca gaatgacttt cagcttcaat
    13801 ggcatcgaag gcacagagac agggagaggg cgcgactcat caaacgcaac ccagtcaaaa
    13861 cgctcgcggt taacgatatt tttcagccgc atccacagca gaggttgcgc cagcagtgta
    13921 cgcaaagaga tgatgagaat accgccattt gcctgatgca ccagaccagg ctgcagggta
    13981 atgtcgccat taaactggcg caggcagcca aagagttgct ccgcttctac ccagtcggca
    14041 gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg acgtaagcgg
    14101 atggagtggc cggaaacctc atagtgatcg cccaccagtt ggcctgcatc gctttgtagc
    14161 gtacgcgcgg catcggcaat aagattcaga tactcagact cttccggggc cttagccagc
    14221 ataaaagagg aggatgctcg cgtatgcagc aactgctcca gcgcaaattg cagccgcggt
    14281 tgagtatcac tgaataaagg atcgttttcg tcaatcaaat ttggctgagc aaatatttcc
    14341 tgatagctat cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat ggtcaaagtt
    14401 gatgtttttt agtctgttgt caaagccgcg attataccgt aaccggcact acagcacacg
    14461 tagaaagcac cgacaatact cctggcatgg gcgttaaagc tcacaggatg gagattcttt
    14521 tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca ttgagatcgc
    14581 tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt atctggtgaa
    14641 gaagcatcgc gaaggggagt taattacccg ttacatagaa gccagtgccg cccaggaagc
    14701 cgtcgatgtg ttgctctcgc tggaaaatga acccgtgctg gtaaatggct ggattgacaa
    14761 gcatatgaat ccggaactgg tcaatcgcat gaagcaaacc attcgggcca gacggaagcg
    14821 ccattttaat gcggaacacc agcatacgcg caaaaagtcg atcgatctgg aatttattgt
    14881 ctggcaacgt ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg aaacgattgt
    14941 tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt cttctctgaa
    15001 gctggatctg caggcattgc tgggtaagga ataactgacg aaagtcagtt caatttacta
    15061 aaggcaaaaa aaaccccgca gcagcggggt ttttctacca gacgataact taagcctgcg
    15121 gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga tccggagcca
    15181 ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt
    15241 tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg gagatcaggt
    15301 aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg tcagaaccga
    15361 tgcggtcggt gtaacccaga acaactacgg aaccgtcttt cggatccagg ttgctcagct
    15421 ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct ttgttgaagt
    15481 tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt gccggagctg
    15541 gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa acacccaagc
    15601 tcagcatgcc gttgtctgga cgagtgccga tggtgtgtgc gtcaccgatg ttgttggtcc
    15661 actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca ccgccagcga
    15721 agaccggaga aacgccggtg tcgtggtttt taccataaac gttggattta gtgtctgcac
    15781 gccataccat accacccaga cgagtgtaga tgtccaggtc gtcagtgatt gggtaaccca
    15841 gtttagcggt cagttgaacg ccctgagctt tgtatgcacc gttttcaacg ctgcctttgt
    15901 acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg ttaacctggt
    15961 aaccaccaaa agcaccagcg cccagttggt tttcatgggt cgggccattg ttgttgatga
    16021 aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag gtgttatctt
    16081 tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc gcgatagctg
    16141 tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat ctccaacgag
    16201 ataacacggt taaatccttc accgggggat ctgctcaata ttaactctac cgatatcttc
    16261 ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa acttacaagt
    16321 gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta atcttttttg
    16381 tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct ccgcaaggaa
    16441 aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga ttaatcctaa
    16501 atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc tcgtggcgtg
    16561 agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat ttgccgcatc
    16621 aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga tcaccacact
    16681 gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag gggagagctg
    16741 gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt cccgactcag
    16801 tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa
    16861 tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa cttcactgat
    16921 aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg cggatgagaa
    16981 cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc ctgtgagtta
    17041 ctgtatgaat atacagtaca tccagtgaca acaaagatca accctatttt cggaaagagc
    17101 ctcgcaaatt ttttcgttgg tgacgggaaa acataaatta atcttgcccc ttaagaataa
    17161 gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta tgttatgatt
    17221 ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca caagaatacc
    17281 tggcaatgtt gggcacaatt gaataccgat cattgtttgg cagttacagc ctgaccgttg
    17341 acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa
    17401 gtgcacagta ctgtgtaaaa catccgcctg tctggctgac atataaaaag tgtggccgat
    17461 ccgttaccct taattactat cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg
    17521 tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc
    17581 gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt ctcggggagt
    17641 ggggattaag gatgtacggg cgttacgtat acttggggca aaaatgtgtt ggttgcgact
    17701 gcggcagcaa aacagtctgg tgacagaaaa gattctgttt atgcttgaag gtgccattat
    17761 cggcattcat gaagctgcgc tcccggtggc acgccgccag gagcttgcag aatgggctga
    17821 ctcacttacg ccgaaacagg agtttcctgc ggaacttgag taatctggcg ttgcagacga
    17881 ccaatctcag gcagtaatgc aatcaataat ccaacttgtt gtacgaccag aggttctttg
    17941 ctgtctgccc gtggttcaag ttgctgcatc cgctgtttca ggctagctaa tgcctcattg
    18001 acgcgttctt catcagcagg ttgatgatgt aacgcgtcat caacatagca aactgcgtca
    18061 tcaagaaacg ccagaatttc aggattagtt aactgctccc ggtgagcacc gagggctgag
    18121 atatagctgg taaacgtatg gttaaggcac agcaaccgaa acgcggcttc gcgaatttgc
    18181 ggggtaacgt tcggctcgct ggacatattt gataccaccg acgccagctc agcatcacgg
    18241 ttgtgtgcat cgcggcgggc aatacgatac gccagacggt tatcacgccc ctgatggtat
    18301 tgctccagta tggcatcgag ataccgacag ttggcctctg tggcgcgttc gagcatgcgc
    18361 ggcaaattgc gaaactgcca gtcaggccag atgtagctca ctgccgccca cgcaatggca
    18421 caaccaatca gcgtatcgat tacgcgaggt aacgctactt caaaaccttc acccagtaag
    18481 ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg catgagcgta ttgcacgtta
    18541 cggaaggcaa aaaagagcac gccggtaata accagcagca ccagctgccc ttccagtgat
    18601 ggcacaaacc acagcacagg aatgccaatg gcgataccta ccagcgtacc aataatcctt
    18661 aacttcaggc ggtggcgcgt ggcgttatag tttggctggc agacaaacaa acttgtcagc
    18721 aagatccaaa acccgtgatg cattccggtt atttgaatga tggcgtagcc gaagcacaac
    18781 accagcgaca ttcttaccgc atgacggaag agggcggatt ccggcgtgaa gtgacggcta
    18841 agacgcagcc agatatcact caacccgtgc gggctgtcat cagcgagctc attttcgtca
    18901 ttattatggg gtagtgcctg ggcctgttct gattcaattg ttgccagttg ggcatcaatg
    18961 gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta aatcggcggg tgcgccgtta
    19021 tcgcgcatcc gctccagcgc agcatcaata tgcgtaaaag cgcgctcaaa atgcggatca
    19081 tgttgataag gctgacgcaa caaaatacag cgtgacagtt gctggcacgc ctggccctgc
    19141 atcgacatca gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg ttcacgcaat
    19201 gtttgatact gaatatgaga agagctggca cgctcgtgaa tatcctgtgc gacaaagtaa
    19261 taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg ggtcagcagc
    19321 gagagtttcg tctgattcaa tgtcgccatc agcagaccgt tggcgagagc caaatcgtac
    19381 agcggtgcct ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt gagctcaaga
    19441 taacgcgcca gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg gaccgggaac
    19501 agcagatgac caataagtgt caggacgttg taccagacgg caccggccag cagatacatc
    19561 ggctgctgat accagtgctc atacagtgat gttcccaaca tagtgtaaat ggcgatcagc
    19621 aatgcaccga aggcaattgt tgcatagcgt tgacccagac cgccgagcaa aatgaagccg
    19681 ctggtagaga gcgttaagcc aatcgcaaat agccagggcc agggaaacag caattctact
    19741 gaggccgagg cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa acgtcccgcc
    19801 agtcggtcat cgagatcggt cagcgccgct gccaccatcc ccagcgttag cggaatcgtc
    19861 agttttacat cacccagcca ccacggaaac gctgtggttc cacaaagcgc aataaaaata
    19921 cgcgcgtaat acagccaggc gctgttccag gtatagcgtt tgagcaaagg acttagcata
    19981 aaggccatat tagtggatta ttcaaaacga cgacgtgcat tggcttcacg cgcagcttgc
    20041 gctgtttcta ccgataccac gcgacgacca accggccata gtgcaatagc ggcaatttta
    20101 aagttcgcaa tgccgacagg aatgccaatg attgaaatac attgtgcgat gcccgttgca
    20161 atgtgcatca ggcataacca ccagccaaag aaaatcagcc agaaaatatt caataccgta
    20221 ccgccagtat tcagcagcac atttttgcca gccgggttca gttcatcgac atgaatagct
    20281 tcattgccat aaggcaccag agacagttta gtgatctccc agcaggatcg tgtcagcggt
    20341 aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca gccagcccag agtggtggca
    20401 aatccgccaa gcacaaagtt cagaatgttc aaaacggtac gcataaaacc tcgctttact
    20461 gtggttttca gtaatgggcg gcaattgtaa cgtttttttg ggctggagca cgttttctct
    20521 gacggttaca ctgataagaa ataatttcgt gtggatctac agagtcatgg aactgaaagc
    20581 gacaacgctt ggaaaacgtc tggcacagca cccttacgat cgggcggtga tcctcaatgc
    20641 cgggattaaa gtctccggcg atcgccacga ataccttatt cctttcaatc aattactggc
    20701 gattcactgt aagcgtggtc tggtatgggg cgagctggaa tttgtactgc cggacgaaaa
    20761 agtggtgcgt ctgcacggca ccgaatgggg cgagacgcag cgtttttacc atcatcttga
    20821 tgctcactgg cggcggtgga gtggcgagat gagcgaaatt gcgtctggtg ttttacgcca
    20881 gcaactggat ttgattgcca cgcgcactgg agaaaataaa tggctgacgc gtgagcaaac
    20941 ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg ttgccgttgc cgttgccggt
    21001 taaccgactg gaagaattcg ataactgccg tgaggcgtgg cgtaaatgtc aggcctggtt
    21061 gaaagatatt gaaagcgctc ggttgcagca taaccaggcg tataccgaag ccatgcttac
    21121 cgagtatgcg gattttttcc gccaggtcga gtcttcaccg ctgaatccgg cgcaggcccg
    21181 ggcagtcgtt aatggcgagc attctctgtt agtgctggca ggtgcaggaa gcggaaaaac
    21241 gtcggtgctg gtggcccgtg caggctggtt gctggcacgt ggtgaagcgt cccctgagca
    21301 aattttattg ctggcgtttg gtcgcaaagc cgctgaagag atggacgagc ggattcgcga
    21361 acggctacat accgaagaca ttaccgcacg cacgtttcat gcgctggcgc tgcatattat
    21421 tcagcagggc agcaaaaaag ttccgatagt cagcaaactg gaaaatgata ccgctgcccg
    21481 tcatgaactc tttattgctg agtggcgcaa gcaatgcagc gaaaagaaag cgcaggcgaa
    21541 gggctggcgg caatggctga cggaagaaat gcagtggtca gtgccagaag gtaacttctg
    21601 ggatgatgaa aaattacagc gtcgccttgc ctctcgcctc gatcgctggg taagtctgat
    21661 gcggatgcac ggtggtgcac aggcagaaat gattgccagt gcacccgaag agattcgcga
    21721 tctgttcagt aaacgtatca agttgatggc cccgttatta aaagcctgga aaggtgcgct
    21781 gaaggcagaa aacgctgtcg atttttcggg ccttattcat caggcgattg tgattctgga
    21841 gaaaggtcgc tttatcagcc cgtggaagca tattctggtt gatgaatttc aggatatctc
    21901 gccgcagcgg gcagcgttgt tagcggcatt acgcaagcaa aacagtcaga cgacgttgtt
    21961 cgctgttggt gatgactggc aggcgattta ccgattcagc ggtgcgcaaa tgtcgctcac
    22021 caccgctttc catgaaaact ttggtgaagg cgaacgttgt gatttagaca cgacttaccg
    22081 ttttaacagt cgtatcggta aggtggcaaa ccgttttatt cagcagaacc caggccagct
    22141 gaaaaagccg ctaaacagct taaccaatgg agacaaaaaa gccgtcacgt tattggatga
    22201 gagtcaactt gacgctttgc tggataagct ctctggttat gccaaaccgg aagagcgcat
    22261 tctgatcctg gcgcgttacc atcacatgag gcctgccagc ctggaaaaag cggcaacacg
    22321 ctggccgaag ttgcaaatcg actttatgac cattcatgcc agcaaagggc aacaggcgga
    22381 ttacgtcatc atcgttggct tgcaggaggg aagtgatggt tttccggctg cggcgcggga
    22441 gtcgattatg gaagaggcgc tactgccacc ggttgaggat ttcccggacg ctgaagaacg
    22501 gcggttaatg tacgtggcgc tgacccgggc acgccatcgg gtatgggcac tgtttaacaa
    22561 agagaatccc tctccctttg ttgaaatact gaaaaatctg gatgtgccgg tggcgagaaa
    22621 accgtaagaa acaggtggcg tttgccacct gtgcaatatt acttcagacg gtccgcgaga
    22681 taacgctgat aatcggggat cagaatatcg accgcgtcgt tgaaatgcgg cgactggatt
    22741 atgaagtctg ccgttgccac gttggtggcg accggaatgt tccataccgt cgccagacgc
    22801 agcaaggctt tcacgtcagg atcgtgcggc acggcattta gtggatccca gaagaaaatc
    22861 aatacatcaa ttttcccttc tgagatcaat gcgccaacct gctggtcacc ccccattggg
    22921 ccactcaaca tcgcgttgac gttcatgccg gtcgcgcggg aaattaagtt accggtagtg
    22981 cctgttgcat acagtacgtg ttgttccagt aacggttgat gccgttccac ccagctcatc
    23041 agcatttgtt tgcagtgatc gtgtgccacc agcgcaatat gtttccgcgc aggtaaagtg
    23101 cgagtcgtca gttccataat gtacatccgt agtcaacttt cctacagatt actgtaagca
    23161 cttatcgctg caagataaag accgaaaaag cctgcgcaca ggcacaaaaa tctcaggaag
    23221 atggttgttt ttccgcccac tgcaggaaag tatttcgcgt ttgtgggtca gccagtttaa
    23281 accaatactt cagccgttgt tctgtgagca cctgagactg cggtggaata gcatccagct
    23341 cggaaacacc cgaaagcagc gtactgtcat ctgccatcat cgtggcaaat tgcggtagtg
    23401 aagcgcgttt cgcggatttg ttatagcgtt caacctcgac ctcataatca atcgtttttg
    23461 ccgttgaggt aatggcgaga atatccagct ttaccggaat cggcgtcgca tcgccatcca
    23521 acaattcaag gcgcggcgcg gcatcaaaat ggttagcttc ccgctcattc tccaggcgag
    23581 gcaggcgaaa attgacctgg ttgatgagct gggtattaaa actgaccact agcggtgggg
    23641 agatatacag ccgttcttca ctattggaaa gatgaatcgt cttctcaacg cgaaacacta
    23701 actgatgcgg tccattatcc agttcaatgc tgtccgcacc gcgcagcaga gagctggaaa
    23761 ccttcttgcc gtcgagtacc agaagatcga catcggttga aagccgcagc gtggtggcaa
    23821 aaacggatac cggcaaacat aatgcaatca aggtggtcac gatgccggtt ttcataggta
    23881 gctcctgtca aaagaccgcc cgaataataa tgtgtcaaaa tttttcttta atcatactta
    23941 ctaacatata gacatatttc ccgcttttgc tctcattcat tcgtattagc tgcatggttg
    24001 gcatgtcggc tttgtcgtac actttgttaa acagtctgga ggaaataatg aaagaaaccg
    24061 atattgcagg cattttaacg tccactcaca ccattgcgct ggtgggggca agcgacaaac
    24121 ccgatcgtcc cagctatcgc gtgatgaaat atctactcga ccaggggtat catgtcattc
    24181 cggtttcgcc aaaagttgcc ggcaaaacgc tgctgggcca gcaggggtat ggcacgctgg
    24241 cagacgttcc ggaaaaggtc gatatggtcg atgttttccg taattcagaa gcggcgtggg
    24301 gcgtggcaca ggaagctatt gccattggcg cgaaaaccct ctggatgcaa ctgggggtga
    24361 ttaatgagca agcggcagta ctggcacggg atgccgggtt gaatgtggtg atggatcgct
    24421 gcccggctat tgagatccct cgcctgggcc tggccaaata aaaaatcccc ggaaggcaaa
    24481 aaccttccgg ggattcgttc agggatcagt tacgcagacg cggggcctgg agttgtttgc
    24541 ggatggtctg cgccagctca tccatggatg gctgttcagg atgttcatcc tgcaattcgc
    24601 tacttagctg ggcttcagcc aggtaggtat gaaccggtag gccgttatcg tcctccatca
    24661 ctacgtgata ccacggagcg gcgcgaagct cgtcatttac cgccagctca tcaggcgacg
    24721 gttcagagag cgaataaacc gggtcgatat ccacgaccac tccgagataa cctaacaggg
    24781 aatggcggac ctgctggccg ataccgaatt tgctggcaat catagtcacc tcccgggaaa
    24841 tctttacctt tgatataagg gtaaaattcc acatttcaag ttacatgacg cgacaggcaa
    24901 accctttcag atatagccct tccggatagg tagcgatcac cggatgatcg gctgcctgac
    24961 ggaactgctc tataaattgt acatcacggc cggcatcaat tgcggcatcc gcgatgattt
    25021 tctgaaataa atcgctggtc atcagaccgg aacaggagaa agtcaggaga ataccgcctt
    25081 cattcagcaa ctgaatcgcc agcatgttga tatctttata gccacgacac gcgcccatca
    25141 actggctttt attctcaaca aacttcggcg ggtccatcac gataacgtca aatttttcac
    25201 cgcgatcgcg ataagtacgc agcaatttaa agacatcatc acggacaaac tcagccttgc
    25261 tcagatccag tttgttcagc tcaacgttct gccgtgcaat atccagcgct tcctgggagg
    25321 tatcaacgct gacaacctgg ctgcaaccgc ccatcagtgc cgatacggcg aaaccaccgg
    25381 tataggagaa acagttcagc acacgtttat tttcaacgta gcggcgggta gccaggcggc
    25441 tgtctcgctg gtccaggtag tagcccgttt tgtgtccgtg ctgaatatcc accagcagct
    25501 tcatgccgtg ttcttcaatc ggcagcaggg caggcggcaa ctcgccggtg acgaggccca
    25561 gggtcagctc catcccttct tttttacgta ccgcaacatc gctgcgatcg taaatcgcac
    25621 attccgggta cagcgtttgc agggcactaa ctaatgccgc gcgctgatat tctgccccag
    25681 cactcagcag ttgcagcacc agaaaattac cgaaacgatc gatagtaata cccggcaggc
    25741 catcagattc tccggcgatt aaacgatagc tgtcgaggcc atctttttgc gccagccagt
    25801 cacgccattt ttgtgcttgt tgcaaacggc gggaaaaaaa agcaatgtcg atagactcag
    25861 acgggtcaaa cgtccagacg cgcgcccgga tttgcgaagc tggcgaataa gcgccgcgtg
    25921 ctaaccattt tccctgatga tcaacaatat cgatggtttc accgaggctg gctttacctt
    25981 ccatgcgggc aacggccccg gaaaagaccc acggatggcg acgaagtaat gatttttcgc
    26041 gccctttggc taacactaaa cgtacactca taatttactt ttctgtcgat gcaaagaaat
    26101 gggcgtcatt gtccggagtt ttccatggat ttgcaacgcg tccattaagg ataaggagag
    26161 cagcaatgtc gaaagtctgc ataattgcct gggtttacgg gcgagttcag ggcgtaggat
    26221 ttcgctacac cacacagtac gaagcgaaaa gactgggttt aaccgggtac gccaaaaatc
    26281 ttgacgatgg cagcgtggaa gtggttgcct gcggtgaaga agggcaggtg gaaaaattaa
    26341 tgcagtggtt aaagagtggc ggcccgcgtt ctgcgcgtgt agaacgggtg ctcagcgagc
    26401 cgcatcatcc ctcgggggaa ttaaccgatt ttcgtatccg ttaaatacat tttaccggtt
    26461 ttggcaggcc agcaattttg gtggcttgct tagccggacc tttcggaaac agtcggtata
    26521 aatagcggct gttacctttt tcttcgccaa atttattcgc catcgctttt accagcatac
    26581 gaatcgccgg agaagtattg aattccagat agaaatcacg cacaaaacgc accacttccc
    26641 agtgttccgg tgacagcgaa atcccttcgt tctctgcaat caccactgcc agcggctcac
    26701 tccactggct gctttctttg agatagcctt cggtatccgt ttctatctct ttaccttcga
    26761 agatcagcat aattattact accttaatca gactggcggc agtgtaacaa aaaacaaagc
    26821 cccgcataaa gcgaggctat gaaagtgtta gcgggtgaga ttaatcgcgg ctggcgaagc
    26881 ccagaatgct cagcaggctg acgaagatgt tgtacagcga aacatacagg ctaaccgtgg
    26941 cacgaatata gttcgtttca ccgccatgaa cgatgttgct ggtttcaaac aaaatagcgc
    27001 cagaggagat caaaatgaag accgcgctga tcgccagatg cagagcaggc agctgcagga
    27061 agatattagc aaccatacca atcagcacca ccacaatacc cgccatcagc ataccgccga
    27121 ggaacgacat atctttgcgg gtggtcagca catatgcaga gcagcagaag aacactaacg
    27181 ccgttccgcc cagagccata gcgattacgt cacccattcc ggcagacaga taggtgttca
    27241 gaataggtcc gaggatataa cccagaaaac cggtaaaggc gaatgcggag ataatcccgg
    27301 tcggcttatt cgccgtttta taggtcagga acatcaaacc atacataccc accagcgtca
    27361 gaatcagacc cggagatggc agcatcagca ccgtactggc agttgcggta atcgccgaaa
    27421 aggccagcgt caggctcagc agaaaatagg tattacgcag caccttatgg gtgctaagca
    27481 gtgatgtacg gtcatgtgaa gaactaacaa tacgatccat tgagtcactc tctatgacag
    27541 atgtaattaa ttaagcagca taatgataat gcgtaagggc acccagaagt tttacccatc
    27601 tttacgcatt tgatctggaa caggtttaac agcggattat caggtcatta agcaaatata
    27661 acgccctgag aatttcgaca ggcaaaagaa aaaggggtta gcatttagct aaccccttat
    27721 cttatttggc ggaagcgcag agattcgaac tctggaaccc tttcgggtcg ccggttttca
    27781 agaccggtgc cttcaaccgc tcggccacac ttccggaatg acgcgcacta taaacatccc
    27841 gatgcggcgt gtaaacccct aatttgtttg tttgcctgaa aaacagccaa aagtgcattg
    27901 atagcgtgaa ataacagcag attgatcatt tcatcaccat gaattccttc tcttttactc
    27961 gtttagcaac cggctaaaca tccccaccgc ccggcaaaaa gaaaaatagg tccattttta
    28021 tcgctaaaag ataaatccac acagtttgta ttgttttgtg caaaagtttc actacgcttt
    28081 attaacaata ctttctggcg acgtgcgcca gtgcagaagg atgagctttc gttttcagca
    28141 tctcacgtga agcgatggtt tgccttgcta cagggacgtc gcttgccgac cataagcgcc
    28201 cggtgtcctg ccggtgtcgc aaggagaaga gacgtgcgat atgaataacg aggaaacatt
    28261 ttaccaggcc atgcggcgtc agggcgttac ccggcgcagc tttctcaaat attgtagtct
    28321 ggctgccacg tcgctgggat taggcgcggg aatggcacca aagattgcct gggcgctgga
    28381 gaacaaaccc cgcattccgg tagtatggat ccacggtctg gaatgcacct gctgtaccga
    28441 atcttttatc cgctccgctc acccactggc gaaggacgtc atcctttccc tgatttccct
    28501 cgattatgac gatactttga tggctgccgc cggaacccag gcggaagaag tcttcgaaga
    28561 catcatcacg caatacaatg gcaaatatat cctcgcagta gaaggtaatc cgccgctggg
    28621 cgagcagggg atgttctgta tcagcagcgg tcgaccgttt attgagaaac tcaaacgtgc
    28681 cgctgccgga gccagcgcga ttatcgcctg gggaacctgc gcgtcctggg gctgcgtgca
    28741 ggccgcgcgg cccaatccga cgcaggcaac gcctatcgac aaagtcatca ccgacaaacc
    28801 cattatcaaa gtacctggct gcccgccgat cccggatgtg atgagcgcca tcattactta
    28861 catggtgacc tttgatcgct tgccagatgt cgacagaatg ggccgtccgc tgatgttcta
    28921 tggtcagcga atccacgata aatgctatcg ccgcgcccac ttcgacgccg gagagttcgt
    28981 ccagagttgg gatgatgacg ctgcccgcaa aggttactgc ctgtacaaaa tgggctgcaa
    29041 agggcctacc acctataacg cctgttcctc cacacgctgg aatgatggcg tttctttccc
    29101 aatccagtct ggtcacggct gcctgggctg tgcggaaaat ggtttctggg atcgcggttc
    29161 gttctacagc cgcgtggtcg atattccgca aatgggtact cattccaccg ccgataccgt
    29221 cggtttaacc gcgcttggcg tggtggcagc ggctgttggt gtgcacgcag tcgccagcgc
    29281 cgttgaccag cgcagacgtc ataaccagca acctacagaa accgaacatc agccaggcaa
    29341 tgaggataaa caggcatgag cactcagtac gaaactcagg gatacaccat caataatgcc
    29401 ggacgccgcc tggtggtcga cccgattacg cgcatcgaag gccacatgcg ctgcgaagtg
    29461 aatattaacg atcagaatgt gatcaccaat gccgtctcct gcggcaccat gtttcgcggg
    29521 ctggagatca tcctacaagg gcgcgacccg cgcgatgcgt gggcgttcgt tgaacgtatc
    29581 tgcggcgtct gtactggcgt acacgccctg gcttcggttt acgccatcga agatgctatc
    29641 ggtattaaag tgccggacaa cgccaatatc atccgcaaca ttatgctggc aacgctctgg
    29701 tgccacgatc atctggtgca cttctatcag cttgccggga tggactggat cgatgtgtta
    29761 gatgcgctga aagccgaccc gcggaaaacc tccgaactgg cgcaaagtct ctcctcttgg
    29821 ccgaaatcat cccctggcta tttcttcgac gtacaaaacc gcctgaaaaa atttgttgaa
    29881 ggcgggcagt tggggatctt ccgcaatggc tactgggggc acccgcagta caaactgccg
    29941 ccagaagcta acctgatggg ctttgcccac tatctcgaag ctctcgattt ccagcgtgaa
    30001 attgtcaaaa tccacgcggt ctttggcggt aaaaacccgc atccaaactg gattgtcggc
    30061 gggatgcctt gcgccatcaa cattgacgaa agcggcgcgg tcggggcagt caatatggaa
    30121 cgcctgaacc tggtgcagtc aattatcacc cgcacggcgg acttcattaa caacgtgatg
    30181 atccccgacg ccttagccat cggtcagttc aacaaaccgt ggagcgaaat cggcactggt
    30241 ctttctgata aatgcgttct cagctacggc gcattcccgg atattgccaa cgactttggc
    30301 gagaaaagtc tgctgatgcc tggcggcgcg gtgattaacg gcgacttcaa caatgtgctg
    30361 ccagtggatt tggttgatcc gcagcaggtg caggagtttg tcgaccacgc ctggtatcga
    30421 tatcccaacg atcaggtcgg gcgtcatccg ttcgatggca tcaccgaccc gtggtacaac
    30481 cccggcgatg tcaaaggcag cgataccaac attcagcagc tgaatgaaca ggaacgctac
    30541 tcgtggatca aagcgccacg ctggcgcggt aacgcgatgg aagtggggcc gctggcgcgc
    30601 acgttaatcg cttatcacaa aggcgatgct gcgaccgttg agtcggtcga tcgcatgatg
    30661 tcggcgttga acctgccgct ttccggtatc cagtcaacgt taggccgcat tttgtgccgc
    30721 gcgcacgaag cgcagtgggc cgcaggtaag ttgcagtatt tcttcgacaa gctgatgacc
    30781 aacctgaaaa acggcaatct cgccactgct tccacggaaa aatgggaacc tgcaacctgg
    30841 ccgacagagt gccgtggtgt cggttttacc gaagcgccgc gcggggcgtt aggccactgg
    30901 gccgccattc gcgatggcaa gattgatctc taccagtgcg tggtgccgac cacctggaac
    30961 gccagcccgc gcgatcccaa agggcagatt ggcgcttatg aagcggcgct gatgaacacc
    31021 aaaatggcga tccccgagca accgctggag atcctgcgta ctctgcacag ctttgacccg
    31081 tgcctcgcct gttcaacaca cgtgctgggc gacgacggta gcgagctgat ctccgtgcag
    31141 gtgcgttaac agcgaaggag aatcatcatg caacagaaaa gcgacaacgt tgtcagccac
    31201 tatgtctttg aagcgccagt ccgcatctgg cactggttga cggtgttatg catggcggtg
    31261 ttgatggtca ccggatactt tatcggcaag ccgctacctt ccgtcagcgg cgaggcgacg
    31321 tatctgttct atatgggcta catcaggtta attcacttca gcgccgggat ggtttttacc
    31381 gtggttttgc tgatgcggat ctactgggct tttgttggca atcgatactc ccgcgagctg
    31441 tttatcgtgc cggtatggcg taaaagctgg tggcagggcg tgtggtatga aatccgctgg
    31501 tatctgtttc tggcaaaacg tccgagtgcc gatataggcc ataatcccat cgcccaggcg
    31561 gcgatgttcg gctatttcct gatgtcggtc tttatgatca tcactggttt tgcgctgtac
    31621 agcgaacaca gccagtacgc tatttttgcg ccgttccgtt atgtggtgga atttttctac
    31681 tggacgggtg gcaactcaat ggacattcac agctggcatc ggctggggat gtggctgatt
    31741 ggcgcgtttg tgatcggtca tgtctacatg gcgctgcgtg aagacatcat gtccgacggc
    31801 acggtgatct ccaccatggt caacggctac cgtagccaca aatttggcaa aataagtaac
    31861 aaggagtgtt catgagcgag caacgcgtgg tggtcatggg gctgggcaac ctgctgtggg
    31921 ccgatgaagg cttcggcgtg cgggtggcgg aacggctgta tgcccattac cactggcccg
    31981 agtatgtgga gattgtcgat ggcggtactc agggactgaa cttgctgggg tatgtcgaaa
    32041 gcgccagcca tctgttgatt ctcgatgcca ttgactacgg gctggaacct ggaacgctgc
    32101 gaacctatgc cggagaacgc attccggctt atctcagcgc gaagaaaatg agcctgcatc
    32161 agaacagttt ctccgaagtg ctggcgctgg cagatatccg cggacatctg ccagcacata
    32221 ttgccctcgt cgggctgcaa cccgcgatgc tcgacgacta cggcggcagc ctgagcgaac
    32281 tggcacggga gcaactgctc gctgcggaac aatcggcgct ggcgcagttg gctgcgtggg
    32341 gaagtgtgcc gcaactggct aatgaatcgc gttgtctcaa ttatgactgt ctgtcgatgg
    32401 aaaattacga aggcgttcgc ttgcgccagt accggatgac acaggaggag cagggatgag
    32461 caacgacacg ccatttgatg cgttgtggca acgaatgctg gcgcgcggct ggacgccggt
    32521 cagtgaatcc cgtcttgacg actggcttac gcaagcgcca gacggcgtgg tgttattaag
    32581 cagtgacccg aaacgcacgc cagaggtcag cgataatccg gtaatgattg gcgaattact
    32641 gcacgagttt cccgactata catggcaggt ggcgattgct gaccttgagc agagcgaagc
    32701 catcggcgat cgctttggcg tctttcgctt tcctaccact ttagtgttta ccggcggaaa
    32761 ctatcgcggc gtgctgaatg gtattcaccc gtgggcggaa ctgataaacc tgatgcgcgg
    32821 gcttgtcgaa ccgcagcagg agcgtgcctc atgagcgaaa cttttttcca tctgctgggg
    32881 ccaggaacgc aaccgaacga tgacagtttc agcatgaatc cactgccgat cacctgtcag
    32941 gtgaatgatg aaccgagtat ggcggccctg gagcaatgtg ctcacagccc gcaggtgatt
    33001 gcgctgttaa acgagttaca acatcaacta agcgaacgcc agccgccgtt gggcgaggtg
    33061 ctggcagtcg atctgttaaa tctcaacgcc gacgatcgtc actttatcaa tacgcttctc
    33121 ggggaagggg aagtgtcagt gcgcattcag caggctgacg acagtaaaag tgaaatacag
    33181 gaggcgatct tctgcggatt atggcgggtg cgcagacgtc gcggcgaaaa gttgctggag
    33241 gacaaactgg aggctggctg cgcgccgctg gcgttgtggc aggcggcaac gcaaaatctc
    33301 ttgccgacag attcgctgtt accgccgccc attgatggcc tgatgaatgg cctaccgttg
    33361 gcgcatgagt tactggcaca tgtacgtaac cccgacgcgc agccgcacag cattaatctg
    33421 acgcaattac ccatcagcga ggctgatcgg ctttttctct cacgtctctg tgggccggga
    33481 aatattcaga ttcgtaccat tggctatggc gagagctata tcaacgccac ggggttacgc
    33541 catgtctggc atttacgctg tacggacacc ttaaaaggcc cgttactgga aagttatgaa
    33601 atctgcccaa taccggaagt ggtgctggca gcgccagaag atttggtcga ctctgcgcag
    33661 cggcttagcg aggtatgtca gtggctggcg gaagctgcac cgacgtaaaa agacggtaag
    33721 tatcgctttc agtcttatga atatcgcaat cggcgaatac ctctggtcgt agagtttcag
    33781 gataaagagg gagatctaac attatcgggt tatttttctc tcttcgccta caggagtgcg
    33841 catgtgggat gtcattgatt tatcgcgctg gcagtttgct ctgaccgcgc tgtatcactt
    33901 tttatttgta ccccttaccc tggggctgat ttttttgctg gctatcatgg aaaccattta
    33961 cgtggtcacc ggcaaaacaa tctaccgcga tatgacgcgc ttctggggta agctcttcgg
    34021 tatcaatttt gctcttggcg tggctaccgg cctgaccatg gagtttcagt ttggtactaa
    34081 ctgggcattc tattccaact atgtgggcga tatttttggc gcaccgctgg cgatggaagc
    34141 attaatggcc ttcttcctcg aatccacctt tgtcgggctt ttcttcttcg gctggcagcg
    34201 gctgaataaa taccagcacc tgctggtgac gtggctggtg gcgttcggtt caaatctctc
    34261 tgcattgtgg atattgaatg ccaacggttg gatgcaatac ccgaccggtg cgcattttga
    34321 tatcgacacc ctacgtatgg agatgaccag cttcagcgag ctggtcttta acccggtcag
    34381 ccaggtgaaa tttgtgcaca ccgtaatggc gggctacgtg accggggcca tgtttattat
    34441 ggcgatcagc gcctggtatt tactgcgcgg acgggagcgc gatgttgcat tacgctcgtt
    34501 tgccatcggt tccgtcttcg gtactctggc gattatcggc acgctacaac tcggggacag
    34561 ttctgcgtat gaagtcgcgc aagtacaacc ggtaaaactg gcggcgatgg aaggggagtg
    34621 gcaaacggaa cctgctcctg caccgttcca tgtggttgcc tggccggaac aggatcaaga
    34681 gcgtaacgcc tttgccatca aaattcccgc gctgttaggg atcctcgcca ctcactcatt
    34741 agataaaccc gtgccgggtc tgaagaattt gatggctgaa acctacccac gcttgcaacg
    34801 cggacgtatg gcctggctgt taatgcagga aatatcgcaa ggcaatcgtg agccgcatgt
    34861 gttgcaggca ttccgggaac tggaaggtga cctgggctac ggcatgttgc tctcccgcta
    34921 tgcgccggat atgaatcatg tcacagccgc acagtaccag gcggcgatgc gtggcgcgat
    34981 acctcaggtt gcgccggtat tctggagttt ccgcatcatg gtgggctgtg gttccctgct
    35041 gctactggtg atgctgattg cgcttgtcca gacgctgcgt ggcaaaatcg accagcatcg
    35101 ctgggtgctg aaaatggcgc tctggagttt gccgttgccg tggattgcga ttgaagccgg
    35161 gtggtttatg accgagtttg gtcgtcagcc gtgggcgata caggacatct taccgacata
    35221 ctccgcgcac tccgctttaa ccacaggaca actggctttc tcactgatca tgatcgtagg
    35281 gctttacacc ctgttcttaa tcgccgaagt ctacctgatg cagaaatatg cccgtctggg
    35341 gccgagcgcg atgcagagtg aacaaccgac gcagcaacag gggtaaagga gaaaatcatg
    35401 tttgattatg aaacattgcg cttcatctgg tggctgctga ttggcgtgat cctggtggtc
    35461 tttatgatct ccgacggatt tgacatgggg atcggctgtc tgctgccgct ggtggcgcgt
    35521 aatgatgatg aacgccggat agtgataaac agcgttggtg cacactggga aggcaaccag
    35581 gtctggttga tcctcgctgg tggggcatta tttgccgcct ggcccagagt gtatgcagcg
    35641 gcgttttccg gcttttatgt ggcgatgatc ctggtgctgt gctcactgtt cttccgcccg
    35701 ctggcctttg attatcgcgg aaaaatcgcc gatgtacgct ggcgtaaaat gtgggacgcc
    35761 ggtctggtca tcggcagtct ggtgccgccg gtagtcttcg gtatcgcctt cggcaacttg
    35821 ttgctcggcg tgccgtttgc cttcacaccg caattacgcg tggagtatct cggcagcttc
    35881 tggcaactgc tgacgccatt ccctttattg tgcggattgc tcagccttgg gatggtaatt
    35941 ttgcaaggtg gcgtctggtt acaactgaaa actgttggtg tgattcatct gagttcacag
    36001 ctggccacca aacgcgctgc actgttggtg atgctgtgct ttttgctggc gggttactgg
    36061 ctgtgggtcg gtattgatgg ctttgtactg ctcgcccagg atgctaacgg tccttccaat
    36121 ccgttaatga aactggtggc agtgctacct ggtgcctgga tgaataattt cgtcgaatcg
    36181 cccgttttgt ggatcttccc gctgctgggg ttcttctgcc cattgctgac ggtgatggcg
    36241 atttatcgtg gtcgcccagg ttggggattt ttaatggcgt cattgatgca atttggcgtg
    36301 attttcacgg caggcatcac gctgttcccc ttcgtcatgc cgtcaagcgt gagtccgatc
    36361 tccagcctga cgttgtggga cagcacttcc agccagctga cgctgagcat tatgttggta
    36421 atcgtgctga tatttttgcc cattgtgttg ctctacactc tctggagcta ctacaaaatg
    36481 tgggggcgca tgacaacaga aactctccgc cgtaacgaaa acgagttgta ctaaggagca
    36541 ggaacaatgt ggtatttact ttggttcgtc ggcattttgt tgatgtgttc gctctccacc
    36601 cttgtgttgg tatggctgga cccgcgtctg aaaagttaac gaacgtaggc ctgatgcggc
    36661 gcattagcat cgcatcaggc aatcaataat gtcagatatg aaaagcggaa acatatcgat
    36721 gaaagcgatc ttaatcccat ttttatctct tctgattccg ttaacctcgc aatctgcatt
    36781 cgctcagagt gagccggagc tgaagctgga aagtgtggtg attgtcagtc gtcatggtgt
    36841 gcgtgctcca accaaggcca cgcaactgat gcaggatgtc accccagacg catggccaac
    36901 ctggccggta aaactgggtt ggctgacacc gcgcggtggt gagctaatcg cctatttcgg
    36961 acattaccaa cgccagcgtc tggtggccga cggattgctg gcaaaaaagg gctgtccgca
    37021 gcctggtcag gtcgcgatta ttgctgatgt cgacgagcgt acccgtaaaa caggcgaagc
    37081 cttcgccgcc gggctggcac ctgactgtgc aataaccgta catacccagg cagatacgtc
    37141 cagtcccgat ccgttattta atcctctaaa aactggcgtt tgccaactgg ataatgtgaa
    37201 cgtgactgac gcgatcctca gcagggcagg agggtcaatt gctgacttta ccgggcatcg
    37261 gcaaacggcg tttcgcgaac tggaacgggt gcttaatttt ccgcaatcaa acttgtgcct
    37321 taaacgtgag aaacaggacg aaagctgttc attaacgcag gcattaccat cggaactcaa
    37381 ggtgagcgcc gacaatgtct cattaaccgg tgcggtaagc ctcgcatcaa tgctgacgga
    37441 gatatttctc ctgcaacaag cacagggaat gccagagccg gggtggggaa ggatcaccga
    37501 ttcacaccag tggaacacct tgctaagttt gcataacgcg caattttatt tgctacaacg
    37561 cacgccagag gttgcccgca gccgcgccac cccgttattg gatttgatca tggcagcgtt
    37621 gacgccccat ccaccgcaaa aacaggcgta tggtgtgaca ttacccactt cagtactgtt
    37681 tattgccgga cacgatacta atctggcaaa tctcggcggc gcactggagc tcaactggac
    37741 gcttcccggt cagccggata acacgccgcc aggtggtgaa ctggtgtttg aacgctggcg
    37801 tcggctaagc gataacagcc agtggattca ggtttcgctg gtcttccaga ctttacagca
    37861 gatgcgtgat aaaacgccgc tgtcattaaa tacgccgccc ggagaggtga aactgaccct
    37921 ggcaggatgt gaagagcgaa atgcgcaggg catgtgttcg ttggcaggtt ttacgcaaat
    37981 cgtgaatgaa gcacgcatac cggcgtgcag tttgtaatgc ataaaaaaga gcattcagtt
    38041 acctgaatgc tctgaggctg atgacaaacg aagaactgtc taatgcgtag accggaaaag
    38101 gcgttcacgc cgcatccggc cactttcagt tttactcttt ctcggagtaa ctataaccgt
    38161 aatagttata gccgtaactg taagcggtgc tggcgcgttt aatcacacca ttgaggatag
    38221 cgcctttaat attgacgcct gcctgttcca gacgctgcat tgacaaactc acctctttgg
    38281 cggtgttcaa gccaaaacgc gcaaccagca ggctggtgcc aacagaacgc cccacgaccg
    38341 cggcatcact caccgccagc atcggcggcg tatcgacaat caccagatcg taatggtcgt
    38401 tcgcccattc cagtaattga cgcatccgat cgcgcatcag cagttcagac gggttaggtg
    38461 gcacctgacc gcgagtaatc acatcaaagc ctcctttgcc aaaatgctgg atcactttgt
    38521 tgagctcatc tttacctgcc agatattccg acaagccatg ttcattactc acggtaaaca
    38581 ggttatgcga ataaccacgg cgtaagtcgg catcaataaa taacactttt tgatcggact
    38641 gggcgatcac cgctgccaga gttgaactga caaacgtttt accactgtct ggcgtcgcac
    38701 cggtgatcat cagaatgtta ttctccgtct ccatcatagc gaagtgcaga ctggttcgta
    38761 gcgcacgtac ggcttccaca gcagaatccg ccgggttatc caccgccagg aaggggatat
    38821 ttttagtacg atggcgctgc tgattagaaa ataaattttt cttacgcaga cgggtacgtt
    38881 tatccagcca ctcggacatt gggatagtgg cataaacgct gatgccgtgc tcttccagtt
    38941 gttccggggc ttctacacca cgacgcaaca tcgcacgcgc cagcacggca cccaccgaaa
    39001 taaacaggcc aagaataaaa ccaagcacca cgttcaacgc ttttttcggt ttcactggct
    39061 gcggctgagt gactgctggg tcgataatcc gcacgttacc aatggcactg gatttcgaaa
    39121 tactcaactc ctgctggcgg ttaagtaatt gcagatatac cgcacggccc gcttctacgt
    39181 cacggcttaa acgtaacact tcctgttgag tggaaggcat tgccgatacc cgcttattca
    39241 ggcgtttgcg ttcttgctcc agcgtctggc gtttttccag cagcgcacga taagttgggt
    39301 gatctttctt atacagctgg gagatctctg cctcgcggaa agtcagctca ttgagttgat
    39361 tatcaacgtt cacaatctgc tcaagaacgg ctttggcttc taggttaagg tcaaccgaat
    39421 cgcgctgctg gcgataaacg ttgagttttt cttccgcctg gtccagctcg ctgcgcactt
    39481 caggcaactg gcgctgtaag aattcaaggc tttgtgaatc ctgcgccgcc tggcgagcga
    39541 tattctgttg caaatagttg ttagcgatgc tgttcagaat acgagtaatc aactggggat
    39601 catcaccagt catggtaagt tccagcatcc cgctttcttt actgcgttcg ctaacggtaa
    39661 aggtttcctg caatgcgtta atcgcttcca gttcggtacg ctggctcagg acaaactgtg
    39721 ttcctggttt ggccttaatg tccgcgatag tcagcgcaac gccatctttt tccagacgct
    39781 ggccgaccat accattgacg gtgaactctt caccttccag tgtatagtgg ccgttttccc
    39841 caaccgtgag tgtcagttgc tgatcctgac cattcagttg tggaatatgc atccagctga
    39901 tcgccagctc acctggtttt tctttggtta atctcgccca gccgcgaccc acaatcggaa
    39961 aatacttctg ctcaactatg tcgtgcagat tcagttcagc aatggtttta ccgagaatca
    40021 tgcgcgattg cagcagttgg atctccggtg cagactcggg cgatgagtta gggatcatat
    40081 cgctcaggcc gctgagaatg gcgttgccct gtttttgctc aacctggacc agagtatctg
    40141 cctgataaat tggtgtgctt aacagcgagt aagcgacagc gatcagcgtg aataacgcgg
    40201 ttacgctgat aataaactta cggtgatccc ataactcgcc gaccagacga agcagatcga
    40261 tctcattttc ctgagtgctg cctggtggcg tattcatatt tttagttgtc atcgtatttt
    40321 ataaactcaa attaccggct gaggcgcttc gcccattcct gactggcgcg ctccaacata
    40381 ccgtagacat gttcaaatgc gtcctgactt ttacgatagg gatccgggat ctctttctgt
    40441 tccagccatt gcccaaacag cattgtcttc ccgcggacct cgggagcgat tgcggtaacc
    40501 tgagcaatat gctctgactc cattgccaga atcagatcgt aatttcgtgc catctcagca
    40561 gtgagcttac gtccggcatg tccttcaaga gatacgccat gattagcggc aacatctgct
    40621 gctgtcgcat ctgcagggtg ttttaccaga ccatgaacac ctgccgattt gacttttacg
    40681 cctggcagac gtttacgcag caagcgctcg ccaatgggcg aacggcaaat gttgccggta
    40741 caaaccacca ggattgagtt aaattttagt tgggccatgt ttgaatccac cgtgaagtct
    40801 ctgtcaggtc atgcaccccg gaaatcgtcg gtaccagcag agaaatcaca cggttccaac
    40861 gagccagcgg cgcggtagtg acataaacaa tgtcgtaagg ttcaagctgg aattccgttc
    40921 cgagtatcat tgccgatgcg tccttcgcat tcagctgata aatattggcg attttgccat
    40981 tctgcttgtt ttgcgttgca cgaataacaa aaatccccgt cgcatcagcg acatcctgat
    41041 tcataccctc agcgttaccc agcgcttccg ccagggtcat accgctgcgg tccattttca
    41101 gcgtgctctg tttacccact tcacccatca cgaagacttt cagatcatcg ttgcgcggaa
    41161 taaataaaat atcgcccggg tgcagcagtt tgttctggcg caagtcgcca cgctgcatca
    41221 gcgcatagag gttcactttg gtctttacgc cattctgggt gagcaccacg ttgcgccagt
    41281 ctgcatctgc ggtcagaccg cccgcggcgt taatcgcatc catgatggtc agaggaatat
    41341 tggtaatcgg ctgctgaccc gatttgctga cttcgccggt gacgtacgct ttttgcgatc
    41401 ggaacgccgc gacgctgaca tcgacttgcg ggctttcaat gactgaatcg agacgcgctg
    41461 taatctcgtt gcgtacctgg gtaagtgttt tcccggccac tttcaggcga ccaatatagg
    41521 ggtagaaaat cgcaccatcg gcgttaaccc agttgccggt atcactggcg ctacggtatt
    41581 gccctgcggg ggtggttagt tccgggtgat cccagaccgt aaccatcaac acatcaccaa
    41641 tcccgatacg atattcatag tttgcgattt gctgctccag ctccgggtta gcctgcgaca
    41701 taatcggttt ggcgcgcaac tgatcaatca atcctggcgt gacgggatag atattgacca
    41761 ttttgtccag gtcatactga ttgtcaggta gttcaatgac atccttgttc ctggtcgata
    41821 aattctgacc aggaacaagg gtacatgctg tcaaagacag aacagccaat gtcagtaccg
    41881 aaaacttaaa aatgttcttt ttcattggtt tatatttatc agtcaatgta atgttatgaa
    41941 ttacgtcagg ttattgtcca accggtactt ctcgctctgc cgtcattgga tagagctggt
    42001 atttgcgtga cagcatttgt ccaccatcac gcgtcaatgg tgtccacgaa accaccgcgc
    42061 ggttgcggtt aggtccaatg gtcatcaagt cgaatggaat cgagatataa aaacctttac
    42121 taaagccgcc ttcgccgtag tcatctttag atacgttact gattgccgcc cataccccta
    42181 ccgccacgcc gctgtcaaag cgtttggcga cgtcgatcgt tgctccttta tcttttgcca
    42241 gatattgccc aacgctaagt ttcatcagta cgccattgag cgtcggcggg ttccagtaag
    42301 ccgtcacgaa accagttggc gtggaataat cggtgaagcg catcatgttg tcccagtcac
    42361 gttgcttcac gtagttaacg tccacaccca gcgcccagct ggcatctagc gggcgataaa
    42421 gcagctcgga accgacaccg gcgtacatcg tttccagata accgccatac acctggccat
    42481 agaaaccatt gcctaagtcg gcaaagtagt tcgcctgcaa gttgttgaga taaacgtcat
    42541 tgcgaacgta atcacggata tgcgtgcgca cgcggggcag ggtagagtcc gcgggcaaca
    42601 gcgaagactt aaacttgtcg tagttgttgt aaatattggt gaaaataccg ccatcaagca
    42661 gcaggtggtc ggtaaaccag tagcgggcac tggacatcag ccccagctgg aacatataga
    42721 aatcttccgg gccgcccagc gactgtgaaa gtgttgggtt gaaagagtag ctaaagcgat
    42781 cttcacgaat acggtagccc cgaccaaagg cagaaagatc ttctgcttca acacgttgtt
    42841 gttgaagttg ctctgattga ccaggcgctg taccagccag ctgtttgcgc aggctggcta
    42901 cgtcggtttc ggtagtcacc atcgccatat gctcgcgctt ttgcgtcacg ctaatcttct
    42961 caacgccttg cggcaggtta ttcaccagaa tccggttggc acgatcgacc gcttcgcgag
    43021 aatcacggta tttgtattgt tgaccagaca tatacagcgt cttatcgcgc agctgaattt
    43081 ccggtgcttc aaaacctgcg ttgtacttca gcgcggtaag ttggttcgct actgtggtgt
    43141 actgcaatcc ttcagattca ggcgcaggtt gatatgccgg ttttggcgta tcgcgcaggg
    43201 cagggcgcag atcgttgaaa ttggtccgta acgtgaagcc aaacatcaac gtgttaccgc
    43261 gttcataact caggttgaga tctgcccagc tggcagcgcg ataaactgcg ccgacgttga
    43321 aatggcttgc ctgaggcagt ttgccagcga aatcattctg gtaattgttg ccatcgtatt
    43381 cgagtttcag acgcagggga ttccacggcg tttgatactc aatgccgcca aagatggaag
    43441 ccgggccacg aaagatatcg ctaaagctga tatcgcccgc atcgtgagac tctgcgcgat
    43501 gacaatattt atcgttcacc cggcaaaacg ggttggtaat attgcccgca ttaccggcgt
    43561 agccccatgc catcccgagg gtgaaatcaa atggccccgc ttgcttgctg gccaccagat
    43621 actcaccgtc aaacagaccc gtaccagcaa tatcacgttt accaaacgcc acttgcggta
    43681 gccagtaacc ttcttcccac aggcgaagtt taaaatcgaa tgatttgtct ttgtatgact
    43741 gatcgccgct gaaatcttcc cactggctat atttgcgtgt gcgcacatcc gtataacgaa
    43801 tggtgccttc cagccatggg aacagcgcca cggaggtgga gtagaaccgg tattgatcgt
    43861 tatcccggta gttgacgctg aattcacctt ccggtgcgat gcgcgcattt ggcatctgca
    43921 acaatcctgt gccgccaaag tctgattgcg acgaacccag cggatccggg taggttaata
    43981 cttcagcatg acaggcactg gagacggcaa tggccaggca gcttaaaaga taagaattct
    44041 tcttcatcag tcaggaacac gttgcgttaa aacagacaca atctggtcat ttaaatcagc
    44101 atatttttcg ggcagtacat gcgcagaaaa gcccaaccaa agctggctgc caggcggtgg
    44161 ttccacatgt cttttattcc acagggcaac aggggcgacg acagtctcac cctcaggcgt
    44221 aatcaccata acgttattct tgtccgcgcc tgcaaggcgg gggtgatctt gcagataatc
    44281 ggtaacgcta cggcctgcct gccagggcaa ttgcccggcc ccagaaaccg cacccagcag
    44341 agtaatagtc accggacgct gtacggtata gagcgtatag tccccgacca acgggggatt
    44401 gctgttctca tccactcgaa caaaatcagg gtccagtttg accggtaagc gaccagtgat
    44461 attgagattg agtaactgct ggcgcacaga tttaatcgtt gccgccacat cgtcgtcagc
    44521 ttctgcttcc caggaagcaa gctgtgccat gacatgttgg tagtctttaa gcgctttagc
    44581 tttcgccgca gagtcggtca gcaaggcacc aggccaccag aggcgatcgc gcagttgcgg
    44641 ttgtgtcacc aactggacaa cattttccat ggggccaacg gaaagtgtct gttgttcgcc
    44701 aggcagataa atagtcaccg ttccttgcgc aaaggcatgg ggtgtcatta cgtaaagtac
    44761 gctggcaatg aaatacgact gtaatttatt catggtgcgg gtttaagaaa cgtcatttct
    44821 acgggaataa cccctgcgcc taacatttgc cgactttgac gaacctggcc agtagccgaa
    44881 tcgacccaga atgtgttttg ccactgtttt tctggccgcg tggaagtcac ctcttcctgc
    44941 cagacattac acagcacatt gcgacccgca atattcagcg tctctaatcc agcaaaggaa
    45001 aaagtagaac tcagggtcgc agagcgaaaa tggttgtctt cactccagag aatatcgcgc
    45061 gtccagcggc tgccatcttt tattgccagc gcgttacgca gagggtcctg cccggaattt
    45121 gtcacttcca gcagattatt attaagtttg acggttttta aaagacgtcc attgtgggta
    45181 accagcatgg cgttatcctg ggataaccat ttgctttgtt cttgttctat atatcccagt
    45241 accacaaaga ttcgttgccc ttcattaagg cgtaaataca tcgtggaata aggcaacgcc
    45301 tggatctgct gatccgctac ggtgatatcc tgattatcga aaaggctggc gcgaaatgta
    45361 tcgaccatac tttgctggct gtgcgtacat cccgcaagaa atagtgcgaa aatcgataaa
    45421 ataagagggc gcacctttaa tccttgaaag gttaattaaa aaaatgcaaa tctttaaagc
    45481 ccggtacgaa tatcgcaacc gggtatcatt taactttaat ttcgacgcgt taaagcagta
    45541 ttagcgtgta gtggacgtgg tggttgaggt gttatgagaa ccgccatcgc cactgtcatt
    45601 tgctgcaaca actgccaacg tcgccacacc aacagccgtt gccacaccga caactgtcgc
    45661 cgtgccgaca gcgccagcag ccacgccacc agtggtagta gcagtcgtgg ttgcgccggt
    45721 agtggtcgtt gtggtgccag tagtacccgt cgttgcctcg gtcccgtttg ttgcttcagg
    45781 ggcagcaaac gcagctggcg tcgtcaacat gaaggccata aggattgcag aaagcttgtg
    45841 tttcataact tttcctttat tcatcgcatg gacaatacgt acgcttgaga tcaggattgt
    45901 ctactttgag tataacatcc ttgtgaaatc tccctttcct cataccgctt gcactatata
    45961 ctgcgggtag gaaaggcgcg cagagggaaa tataagattg ttaactatcc ccctctggaa
    46021 atacgacttg tgagcataag ataaatctta gcaaaataca aattaaagat aaggaaattg
    46081 ttacgaaagc tattaattat tgctggtaat atagtttcaa gtggtactat aactggctgt
    46141 taaatggcat ttataactat taggtgcaga gatattcgct taaatggatt agtttctttg
    46201 tggaaaagta actgatgtta taatatatga tgttgactat tgcactattc caataaaacc
    46261 agtcagcttt aaacaagcag cgtcatatta agagagataa acatttgccg ctgttggtcc
    46321 tcgcaggcca tttacgcggc aaaattccac acgtaatcct ggtataagca cttctgcgtc
    46381 gcggggagtg aatgcggaaa tatggacctg aacttcttta cgaccgtcgg aggggataat
    46441 gaatcctttg ccgcttttgc gatcaaaggt tttgacaatt cctgtcattt tacgggacaa
    46501 acaaattcct tactgaaaat actgcgctgc actatacggg gttaataaaa taaagccagc
    46561 gatatttaag accgccggac ggctaaaata aaatttgctt aatctcaatt atcatgcgtt
    46621 aatagctgcg tcggtttgaa agacagacag catacaaagt agtttactaa agcagttctc
    46681 attatcaggc attatcccct tcttttgagt ctctctcctg aacactaagt agtttctgta
    46741 ttaaagccct gtttgccgaa aggcccaaaa tgaaggaagt aaaatatgtc taataaaatg
    46801 actggtttag taaaatggtt taacgcagat aaaggttttg gctttatcac tcctgatgat
    46861 ggcagcaaag acgttttcgt ccatttcact gccatccaga gcaatgaatt ccgcacgctg
    46921 aacgaaaatc agaaagttga attttctatt gagcaggggc aacgtggccc cgcggcagcg
    46981 aacgttgtta cgctctaagg ttgccgttat tactcaacat ctccatttcc gctgtccatg
    47041 ttgtcatggt tcacagtacc gcacatcggc attcgatgtg acggagcgaa atcctttggg
    47101 cgctaagtgt attttttgta aatcgacgat gatcaccttt gataacgtca cgctgcaaat
    47161 acgcactgac catgcgccgc tggatttcac aaaataatat caggctccct cg
//
LOCUS       DAJTOR010000007        45260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTOR010000007 DAJTOR010000000
VERSION     DAJTOR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.007"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1886
                     /gene="fhuE"
                     /locus_tag="OO249_000403"
     CDS             <1..1886
                     /gene="fhuE"
                     /locus_tag="OO249_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCR5634820.1"
                     /translation="ENTLEISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLG
                     DALSDMALFERVEVVRGATGLMTGTGNPSAAIDMVRKHATSREFKGDVSAEYGSWNKE
                     RYVADLQSPLTEDGKIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAG
                     YEYQRIDVNSPTWGGLPRWNTDGSSNSYDRARSTAPDWAYNDKESNKVFMTLKQRFAD
                     TWQATLNATHSEVEFDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRK
                     VDALDLFADGSYELFGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQ
                     TDWSPQSLAQDDTTHMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTP
                     YAGLVFDINDNWSTYASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSGWMNSRLTT
                     TLAIFRIEQDNVAQSTGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGA
                     TRYIAEDNEGNAVNPNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGT
                     FRAEQGSYALVDLFTRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGT
                     YQF"
     gene            complement(1946..3379)
                     /gene="ptsG"
                     /locus_tag="OO249_000404"
     CDS             complement(1946..3379)
                     /gene="ptsG"
                     /locus_tag="OO249_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5634821.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMHDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(3674..4471)
                     /locus_tag="OO249_000405"
     CDS             complement(3674..4471)
                     /locus_tag="OO249_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5634822.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(4482..5486)
                     /gene="holB"
                     /locus_tag="OO249_000406"
     CDS             complement(4482..5486)
                     /gene="holB"
                     /locus_tag="OO249_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5634823.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(5483..6124)
                     /locus_tag="OO249_000407"
     CDS             complement(5483..6124)
                     /locus_tag="OO249_000407"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5634824.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(6114..7136)
                     /gene="yceG"
                     /locus_tag="OO249_000408"
     CDS             complement(6114..7136)
                     /gene="yceG"
                     /locus_tag="OO249_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5634825.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(7139..7948)
                     /gene="pabC"
                     /locus_tag="OO249_000409"
     CDS             complement(7139..7948)
                     /gene="pabC"
                     /locus_tag="OO249_000409"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5634826.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(8068..9309)
                     /gene="fabF"
                     /locus_tag="OO249_000410"
     CDS             complement(8068..9309)
                     /gene="fabF"
                     /locus_tag="OO249_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5634827.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(9397..9633)
                     /gene="acpP"
                     /locus_tag="OO249_000411"
     CDS             complement(9397..9633)
                     /gene="acpP"
                     /locus_tag="OO249_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5634828.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(9844..10578)
                     /gene="fabG"
                     /locus_tag="OO249_000412"
     CDS             complement(9844..10578)
                     /gene="fabG"
                     /locus_tag="OO249_000412"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5634829.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(10591..11520)
                     /gene="fabD"
                     /locus_tag="OO249_000413"
     CDS             complement(10591..11520)
                     /gene="fabD"
                     /locus_tag="OO249_000413"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5634830.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(11536..12489)
                     /gene="fabH"
                     /locus_tag="OO249_000414"
     CDS             complement(11536..12489)
                     /gene="fabH"
                     /locus_tag="OO249_000414"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5634831.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVANQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(12557..13627)
                     /gene="plsX"
                     /locus_tag="OO249_000415"
     CDS             complement(12557..13627)
                     /gene="plsX"
                     /locus_tag="OO249_000415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5634832.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLRPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(13708..13881)
                     /gene="rpmF"
                     /locus_tag="OO249_000416"
     CDS             complement(13708..13881)
                     /gene="rpmF"
                     /locus_tag="OO249_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5634833.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(13933..14454)
                     /gene="yceD"
                     /locus_tag="OO249_000417"
     CDS             complement(13933..14454)
                     /gene="yceD"
                     /locus_tag="OO249_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5634834.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            14653..15237
                     /gene="yceF"
                     /locus_tag="OO249_000418"
     CDS             14653..15237
                     /gene="yceF"
                     /locus_tag="OO249_000418"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5634835.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASGYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLCEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(15349..16308)
                     /gene="rluC"
                     /locus_tag="OO249_000419"
     CDS             complement(15349..16308)
                     /gene="rluC"
                     /locus_tag="OO249_000419"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5634836.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            16594..17613
                     /locus_tag="OO249_000420"
     CDS             16594..17613
                     /locus_tag="OO249_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR5634837.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
     gene            18324..21509
                     /gene="rne"
                     /locus_tag="OO249_000421"
     CDS             18324..21509
                     /gene="rne"
                     /locus_tag="OO249_000421"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5634838.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(21706..22659)
                     /gene="flgL"
                     /locus_tag="OO249_000422"
     CDS             complement(21706..22659)
                     /gene="flgL"
                     /locus_tag="OO249_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR5634839.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFAIQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLAVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(22671..24314)
                     /gene="flgK"
                     /locus_tag="OO249_000423"
     CDS             complement(22671..24314)
                     /gene="flgK"
                     /locus_tag="OO249_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR5634840.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(24380..25321)
                     /gene="flgJ"
                     /locus_tag="OO249_000424"
                     /pseudo
     CDS             complement(24380..25321)
                     /gene="flgJ"
                     /locus_tag="OO249_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            complement(25321..26418)
                     /gene="flgI"
                     /locus_tag="OO249_000425"
     CDS             complement(25321..26418)
                     /gene="flgI"
                     /locus_tag="OO249_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5634841.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVSGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(26430..27128)
                     /gene="flgH"
                     /locus_tag="OO249_000426"
     CDS             complement(26430..27128)
                     /gene="flgH"
                     /locus_tag="OO249_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5634842.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(27181..27963)
                     /gene="flgG"
                     /locus_tag="OO249_000427"
     CDS             complement(27181..27963)
                     /gene="flgG"
                     /locus_tag="OO249_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5634843.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMQQKLTQL"
     gene            complement(28101..28856)
                     /gene="flgF"
                     /locus_tag="OO249_000428"
     CDS             complement(28101..28856)
                     /gene="flgF"
                     /locus_tag="OO249_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR5634844.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(28876..30084)
                     /gene="flgE"
                     /locus_tag="OO249_000429"
     CDS             complement(28876..30084)
                     /gene="flgE"
                     /locus_tag="OO249_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5634845.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATPQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(30109..30804)
                     /gene="flgD"
                     /locus_tag="OO249_000430"
     CDS             complement(30109..30804)
                     /gene="flgD"
                     /locus_tag="OO249_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5634846.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTMLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAITASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(30816..31220)
                     /gene="flgC"
                     /locus_tag="OO249_000431"
     CDS             complement(30816..31220)
                     /gene="flgC"
                     /locus_tag="OO249_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR5634847.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(31224..31553)
                     /gene="flgB"
                     /locus_tag="OO249_000432"
     CDS             complement(31224..31553)
                     /gene="flgB"
                     /locus_tag="OO249_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5634848.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTLGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYQMSLSALSGQIKGMM
                     NVLQSGN"
     gene            31709..32368
                     /gene="flgA"
                     /locus_tag="OO249_000433"
     CDS             31709..32368
                     /gene="flgA"
                     /locus_tag="OO249_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5634849.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARMRMVSGLVVSGVVDADGNILIN
                     L"
     gene            32444..32767
                     /gene="flgM"
                     /locus_tag="OO249_000434"
     CDS             32444..32767
                     /gene="flgM"
                     /locus_tag="OO249_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5634850.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTDSTSTSTSTS
                     TSTSTSVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADALINEAQQ
                     DLQSN"
     gene            32772..33188
                     /gene="flgN"
                     /locus_tag="OO249_000435"
     CDS             32772..33188
                     /gene="flgN"
                     /locus_tag="OO249_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5634851.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(33228..34763)
                     /gene="murJ"
                     /locus_tag="OO249_000436"
     CDS             complement(33228..34763)
                     /gene="murJ"
                     /locus_tag="OO249_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5634852.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(34930..35796)
                     /locus_tag="OO249_000437"
     CDS             complement(34930..35796)
                     /locus_tag="OO249_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5634853.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAAPDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(35798..36445)
                     /locus_tag="OO249_000438"
     CDS             complement(35798..36445)
                     /locus_tag="OO249_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5634854.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAELESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(36456..37040)
                     /gene="rimJ"
                     /locus_tag="OO249_000439"
     CDS             complement(36456..37040)
                     /gene="rimJ"
                     /locus_tag="OO249_000439"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5634855.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            37276..38484
                     /gene="mdtH"
                     /locus_tag="OO249_000440"
     CDS             37276..38484
                     /gene="mdtH"
                     /locus_tag="OO249_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5634856.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            38548..39195
                     /gene="grxB"
                     /locus_tag="OO249_000441"
     CDS             38548..39195
                     /gene="grxB"
                     /locus_tag="OO249_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5634857.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            39329..39889
                     /locus_tag="OO249_000442"
     CDS             39329..39889
                     /locus_tag="OO249_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5634858.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            39995..41041
                     /gene="pyrC"
                     /locus_tag="OO249_000443"
     CDS             39995..41041
                     /gene="pyrC"
                     /locus_tag="OO249_000443"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5634859.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            41115..41360
                     /gene="dinI"
                     /locus_tag="OO249_000444"
     CDS             41115..41360
                     /gene="dinI"
                     /locus_tag="OO249_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5634860.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGVTKEDKQRISEILQETWESADDWFVSE"
     gene            41650..41904
                     /gene="bssS"
                     /locus_tag="OO249_000445"
     CDS             41650..41904
                     /gene="bssS"
                     /locus_tag="OO249_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5634861.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            42019..43137
                     /gene="solA"
                     /locus_tag="OO249_000446"
     CDS             42019..43137
                     /gene="solA"
                     /locus_tag="OO249_000446"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5634862.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCTGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            43185..43298
                     /locus_tag="OO249_000447"
     CDS             43185..43298
                     /locus_tag="OO249_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5634863.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            43559..44125
                     /locus_tag="OO249_000448"
     CDS             43559..44125
                     /locus_tag="OO249_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5634864.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFTLGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            44129..44704
                     /locus_tag="OO249_000449"
     CDS             44129..44704
                     /locus_tag="OO249_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5634865.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGNDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(44746..>45260)
                     /locus_tag="OO249_000450"
     CDS             complement(44746..>45260)
                     /locus_tag="OO249_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5634866.1"
                     /translation="LPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGFNKVWHI
                     EGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDSHTNCKN
                     DGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNKSRGRLN
                     TTLGIPDPTE"
BASE COUNT        11052 a        12086 c        11266 g        10856 t
ORIGIN      
        1 tggaaaacac gctggagatc agcaaaagtc aggcggattc cgatcgtgct ctttattatt
       61 cccgcggatt ccagatcgat aactatatgg ttgatggtat ccccacctat tttgaatcgc
      121 gctggaatct gggcgatgca ctttctgata tggcactgtt tgaacgcgta gaagtagtgc
      181 gtggcgcgac aggactcatg accgggacgg gtaatccatc tgcggcaatt gatatggttc
      241 gaaaacacgc gaccagtcgt gaatttaaag gcgatgtctc ggcggaatac ggtagctgga
      301 acaaagaacg gtatgtggcg gatttacaaa gcccactcac cgaagacggt aaaatccgcg
      361 cgcgaattgt cggcggctac cagaataacg actcatggct ggaccgctac aacagtgaaa
      421 agaccttctt ctccggcatt gtcgatgctg atttaggcga tcttacgacg ctttcagccg
      481 gttacgaata tcagcgcatt gatgttaata gccctacctg gggcggttta ccgcgctgga
      541 atactgatgg cagcagcaac agttacgatc gtgcacgcag taccgcacct gactgggcgt
      601 acaacgataa agagagcaac aaggtcttta tgaccctgaa gcagcggttt gctgatacct
      661 ggcaagcgac actgaatgcc acccactctg aagtcgaatt tgacagcaaa atgatgtatg
      721 tcgatgccta tgtaaacaaa gcggatggta tgctggttgg gccatacagt aattatggac
      781 ctggctttga ttatgtcggc ggcaccggtt ggaacagtgg caaacgtaaa gttgatgcgc
      841 tggatttgtt cgctgacggt agttatgaat tgtttggtcg tcagcacaat ctaatgtttg
      901 gtggcagtta cagcaaacaa aacaatcgtt acttcagttc atgggccaac atcttcccgg
      961 atgaaattgg cagtttctac aactttaatg gcaatttccc acaaaccgac tggtcaccac
     1021 agagcctggc acaggacgat accacacata tgaaatcgtt atatgctgcc actcgagtca
     1081 cccttgccga tccgctgcat ctgatcctcg gcgcacgtta taccaactgg cgggttgata
     1141 cgctgactta cagcatggag aaaaaccaca ccacgcctta cgctggtctg gtgtttgaca
     1201 tcaatgacaa ctggtcgacc tacgccagct atacctctat tttccagccg caaaatgatc
     1261 gtgacagttc aggcaaatat ctggctccaa tcaccggtaa caactacgag ctgggtctga
     1321 aatcgggctg gatgaatagc cgtctgacca ccacgttagc catcttccgt attgagcagg
     1381 ataatgtcgc tcagtccacc ggtacaccta tccccggcag caacggcgaa accgcctata
     1441 aagcggtgga tgggacagtc agtaaagggg tggaatttga actcaacggc gcaattaccg
     1501 acaactggca gctgacattt ggcgcaacgc gctatattgc agaggataac gaaggaaacg
     1561 ccgttaatcc taatctgcca cgcaccacgg ttaaaatgtt caccagctat cggttgcctg
     1621 tcatgccaga gttgacagtc ggcggtggtg tgaactggca aaatcgcgtg tataccgaca
     1681 ccgtgacacc gtatggcacc ttccgcgccg agcaaggtag ctacgcgctg gtggatctct
     1741 tcacccgcta ccaggtgacg aaaaacttct cgttacaggg gaacgtcaat aacctgttcg
     1801 acaaaaccta cgataccaac atggaaggtt ctatcgtcta cggcgcaccg cgtaatttca
     1861 gcattaccgg cacgtatcaa ttctgaataa cacctgtaaa aaaggcagcc atctggctgc
     1921 cttagtctcc ccaacgtctt acggattagt ggttacggat gtactcatcc atctcggttt
     1981 tcaggttatc ggatttagta ccgaaaatcg cctgaacacc agaacccgca accactacgc
     2041 ccgctgcgcc cagtttcttc aggccagcct gatccacttt agacacatca gcaacgctga
     2101 cgcgcagacg ggtaatacat gcgtcgaggt tagtaatgtt ttctttacca ccaaatgcag
     2161 caaccagagc cggtgccatt tcgctggtac ctgttgcttt tgcatcttca gtcgcgtctt
     2221 cacgacccgg cgttttcaga tccagtgctt taatcagcac gcggaagatg gtgtagtaaa
     2281 caatcgcata accgataccg acgatcggga acagccacag tttgctgctg ttaccagaca
     2341 gaacgataaa gtcgatcaga ccgtgcgaga acgacgtacc gtcatgcatc cccagaagaa
     2401 tacagattgg gaatgccagg cctgccagaa tcgcgtggat gatgtacagg atcggcgcaa
     2461 cgaacatgaa ggagaactcg atcggctcgg tgataccggt caggaacgag gtcagcgccg
     2521 cggagatcat aataccgccc actttcgcgc ggttttctgg tttagcagag tgccagatag
     2581 caattgcggc agctggcaga ccgtacattt tgaacaggaa gccaccagac agtttacccg
     2641 cagtcgggtc acccgccata taacgcggaa tgtcgccgtg gaaaacctga cctgctgcgt
     2701 tggtgtattc accaatctgc atctggaaag gtacgttcca gatgtggtgc agaccaaacg
     2761 gtaccaggca acgttcgatg aaaccgtaaa tgccaaacgc aactaccggg ttctggtaag
     2821 cagcccactg agagaaggtc tggattgcag aaccaatcgg cggccaaatg aaggacagca
     2881 caacgccagt aaagatggca gccaggccag aaatgatcgg cacaaagcgt ttaccggcaa
     2941 agaagccaag atactcaggc agcttaatac ggtagaaacg gttaaacatg tacgctgcga
     3001 tcgcaccgga gataatccct ccgagtacgc cagtatccgc taggtgttta gaggcgattt
     3061 cttcagcagg taaatgcagt accagtggcg caaccacggc catggtttta accatgatgc
     3121 cataggcaac aactgcggcc agcgcggata cgccatcgtt attggtaaag ccgagggcga
     3181 caccgatcgc aaaaatcagt ggcatgtttg caaagacgga accgcctgct tctgccataa
     3241 catgcgatac aacggcgggc agccagctga aattcgcgga accgacgccc agcagaatac
     3301 ctgcgatagg tagtacggat accggcagca tcagcgattt accgaccttt tgcaggttag
     3361 caaatgcatt cttaaacata attgagagtg ctcctgagta tgggtgcttt ttttacgttc
     3421 tcacgcgtgg caagggggga gagcctcgcc gtgtacaggg catctaagcg ccctttattt
     3481 attacacaga gtaaaataat tcagtgccaa tttgtttgac ggctatcacg tttcaacttc
     3541 agatcattgg agaagttgca cattttgaca aaacatatgt cacaatattt caaacgcggt
     3601 tattcaccgc ccaattttcg gcggtgaact ttactcgttt tagccattaa ttacgagctt
     3661 taaaaaaact cattcaacgg atggattgaa ggcgggaagc gtcgatgtga aacagacggg
     3721 cgaagttatc ggtggttacc tgcgccagtt cttcaacggc aacacctttc aacacagcca
     3781 tgtattctgc aacgtcacga accatcgcag gttgattctc ttttcctcga tgcggtaccg
     3841 gcgcaaggta aggtgagtca gtttccacca gtaatcgatc cagggggaca taacgcgcag
     3901 catcgcgcag ttgctccgca ttacggaagg tcacaatgcc ggaaaaggag atgtaaaatc
     3961 cgagatccag taatttaccc gccgtttctc tgtcctctgt aaaacagtgt agtacgccac
     4021 cgcaatccgt cactttttct tcgcgcagaa ttgccagcgt atcggcacgg gcgtcacggg
     4081 tatggacgat aaccggcttg ttcagttcac gaccaatctg gatatgatgg atgaaggact
     4141 cttgctgacg tactttagtt tccggtgtgt aataataatc cagcccggtt tcacccagcg
     4201 ctacaacacc ctcttctgcc gccagacggc gtaaatcttc tacatcgtag ggatcattct
     4261 ggttgagtgg atgcacgcca caagaaaata cgacgttgtc acgttcgcct accagatccc
     4321 gcatatgtaa ataacccggt aatgttgtgg cgactgccag acaaaatttc acatcgcgtg
     4381 cggcggcttt cgccagaacg tcatccacgt ccttatgcaa agattcataa tccagaccat
     4441 cgagatggca gtgtgagtcg actaaaaaca tgatgtctct cttaaagatg aggaaccggt
     4501 agcacaacgc ccggttgcag gtaatgctca atacgcagta aaagatcggt gatgagaagc
     4561 tcgcggttga tgcctgtgac agacattaac tgttcacgaa tgtggcaaac atcccccagt
     4621 atagcctgca ggcgcgaggg agaaagatgg tttgccagtt cggcgaccag gcccggcaca
     4681 tcaacattgg tcacctgtgc agcgccatga tggcgtttta gcgcatccat cagcaacgtt
     4741 gccagccagt gtaaacgcgc cggagcttgt tcatgattaa gggccgccag cagcgaatac
     4801 cagtcgcccg atggtacgct atatgccaac gcctgacaca atgtttcacg agcctgccag
     4861 ttatctccct gaaacaacgc cagtgccgcg ctaggcgaac cggcacttaa gcgcaatgcg
     4921 gcaagtaatg catcctgtga cattgtcact tcgcgtgaaa gccaggtcac ggcgtactgt
     4981 tccggcggcg gcgcaaggta atgtaaccga caacgactac gtaatgttgc cagtaaacgt
     5041 tcaggctcgc gggtagccag gaaaaaccag gtttctgctg gtggctcttc aagcgttttc
     5101 agcaatgcgt tagccgcggc gtcggtcagt aaggcagcat cggttaccca gacaactttc
     5161 gcaccaccta ggcgtgcgtg ctcattcagc ttttcggtga cctcacgtac cgcatcaatg
     5221 cccagcgtat ttttcccttt ttcgggagcc agggtgtagt aatcgggatg cgtgccagcc
     5281 tgcatcaact gacatccacg acagtgaccg caacttttgt ggccctgcgg ttgttggcag
     5341 agtaaataac ggctcagggc gtagattaaa gcatcatcgc ccatgcccgg taacgcctga
     5401 atgagtagcg catggtgacc tcttccggcc tgatagctgg ctaccagttt ttcgaaatca
     5461 ggtcgtaacc atggatacca tctcatgcgt ccaactcctt cacccagtgg gtcacggtag
     5521 tgcggattgc atccatcacg gcctccagcg gctgggtggc atcaatggta tgaatacttt
     5581 tatcttgtgc agccagttcc agatagcggg cgcgggtgcg attaaagaaa tcgaaagact
     5641 cttgctcaat acgatccagc tcgccgcgcg cacgcgcgcg ttttaagcca acttccgggg
     5701 taacatcgag atagagcgtt aagtcagggc gaaaatcccc gagaacagca tcacgcagtg
     5761 ttgccagcat atgttggtca ataccacgtc ctccgccctg atacgcctga gtggagagat
     5821 cgtggcgatc gccaatcacc caggtgccgt tagccagagc tggtttgatg accgtttcta
     5881 ccagttgaac gcgcgcggca taaaacatca gaacttcggc tttatcggta atgacttcat
     5941 cgcctaccga tttgatatcc agcaccaggc ttcttaactt ttcggcaagt tgcgtaccgc
     6001 caggttcccg agtgaaaacc atgtcgcgga tacccagttg ctcaagcgtc tcaaccacca
     6061 cattacgcgc ggtagttttg cctgcgcctt ccagcccctc aatgacgata tacttactgc
     6121 gcattttttt ccttaagcac tttcagataa tcctgcacag acttgttatg gctggcaaga
     6181 ttggtattaa acgtgtgacc acctttacca tcggccacaa aatagagata cggcgttttt
     6241 gccggatgcg cagcagcctt cagcgaatcc gcccccggcg tagctatcgc acctggcggc
     6301 aaaccggtaa tggtataggt gttatacgct gtcggcgttt ccaggtctgc acgagaaagt
     6361 ttgccattat aacgctctcc catcccgtaa atcacggttg ggtcggtctg caggcgcata
     6421 ccaatgcgta aacggttgat aaataccgag gcaacctgat cgcgttcact ggcaacggcg
     6481 gtttcttttt cgataattga tgccatcgtc accagctggt ttttatcttt ataaggcagg
     6541 ccgtccgcac gcccttccca ggcgctatcg accgctttca ccattttctt gtgcgctcgc
     6601 ttgagtaacg cgacatcggt ggtattggcg gtatacatcc aggtgtctgg ccagaaccaa
     6661 ccttcaatcc actccgggtt ttccagttca agtgcctgcg ctacggtggc gtacttatcg
     6721 tcactcagcg tgtgcttgat atacggagcc tcacgcaatt gcttgaggta gtcgctcaga
     6781 cgcatccctt ctaccagtcg cagcgggaac tgtgcttctt taccgctttc cagcaatttc
     6841 agcatctcgc gcacggtcat ctgcggtgta aagcggtaag tcccggcttt aaagtgagaa
     6901 agatccggtt cgatacgcag caaccattga aaaacccgtg ggcgattgat gatcttatcg
     6961 gcataaagct gttcaccaag cgccagacgt ccggtccctg gcttcagggt aaatatcgtc
     7021 tcttctttga taagcaattt gctgtcggca agatggcgaa ccttccagac gcccacacca
     7081 gcggcgatac ccagtaccac cagcaataac aagataatca ataacacttt tttcatgact
     7141 aattcgggcg ctcacaaagt ggggctaaat attcatataa cgttgctgac gaaaaggaga
     7201 catcgccaca ggcacgtacg ggcatcactg gcattaacgc attacaaata accatctcat
     7261 ctgcctgcaa cgcctcttcc agagaggctt gcacttcgac aagctgataa gaggattgtg
     7321 ccagcaaacg gatacagaat tgtcgcataa tgccgttaac acctgcctga tccagtcgcg
     7381 gcgtataaac tacattgccc ttccgccaga acaaattagc cgcacagcat tccgtaaccc
     7441 acccttcgct gtcaaggacc agcgcctcat cagcgtttgt ctgctcaaga tgagagcgga
     7501 tcaatacttg ctcaagccga ttaagatgtt taatacctgc aagatgggga ttgcgcccca
     7561 gccgcaccgg gcttagcgcc aacgtcatcc cttcgttacg caaacggtcg taatgtgcag
     7621 gataaaccgt aacggagaga atccgcgttg ccggtccact gttcaatgtg ctgtaccctc
     7681 gcccgccact accgcgactg atcacgactt tcagcacacc attttgctgt tctgctgcca
     7741 gcgttttcat ctcttgttca agctgaggcc agaagtcaca gaaaatcatc aaccgctgac
     7801 aagcatcctg tagtcgctgg atatgcgccg ataacaaact gactttaccg tcgataactc
     7861 tggcggtggt aaaacaacca tcaccaaact gcgttgcccg atcgcttact gccagcgatt
     7921 cctgcttatg accgttaatt aagaacatac cggctcctta tcagagaatt gttagtgtgg
     7981 cagcatgctc actacggaac aagtcggaat aaaagctaag aaaaaaggcc cgcaagcgga
     8041 ccttttatat gggtgggaaa tgacaactta gatcttttta aagatcaaag aaccattagt
     8101 gccaccgaag ccgaaggagt tacacagcgt gtattccatt ccgctgacct gacgtgcttc
     8161 atgcggtacg aaatccagat cgcaaccttc atccaggtta tccaggttga tggttggcgg
     8221 aacagcctga tcgcgcagcg ccaggatgga gtagatagac tctactgccc ccgccgcacc
     8281 taacaggtga ccggtcatag atttcgtgga gcttaccaac acacggcttg cagcttcacc
     8341 gaagatggct ttcaccgcct gcgcttcagc tttatcgcca gccggcgtag aagtaccgtg
     8401 cgcgttaacg tagccaatct gactcgcttc aatgcctgca tcacgcagag catttgccat
     8461 tgccagagct gcgcctgcgc cattttctgg cggtgacgtc atatgataag catcgctgct
     8521 cataccaaag ccgacgagtt cagcgtaaat tttcgcaccg cgttttttcg cgtgttcgta
     8581 ctcttctagt accagcatac cggcaccatc gcccagtacg aagccatcac gctctttatc
     8641 ccacgggcgg ctcgccgctt gcgggttatc attgcgggta gataatgcac gtgccgcgcc
     8701 aaaaccacca acgcccagcg gcgtactggc tttctctgcg ccacctgcaa ccatcacgtc
     8761 agcatcgcca tacgcgataa tacgcgcagc atgaccaatg ttgtgcacgc cggaagtaca
     8821 ggcggtcgcg atagagatgc ttgggccacg caggccatac atgatagtca gatgacctgc
     8881 caccatgttc acaatcgttg acggaacgaa gaatgggctg atcttacgtg gaccaccgtt
     8941 catcagagat gtgtggtttt cttcgatcag tccgaggccg ccaatcccgg agccaattgc
     9001 ggcaccaatg cgggttgcgt tctcttccgt tatttcaagg ccagaatcct gcatggcctg
     9061 aacgccagcg acaattccat attgaatgaa ggcatccatc ttgcgctgtt ctttgcgcga
     9121 gataatgtcc tcacagttaa aatcctttac taagccagca aatttcgttg cataggcgct
     9181 agtatcgaaa tggtcgatta ggctgatgcc actctgaccg gcaagcagag ctttccaggt
     9241 agactctacg gtattgccga caggagacaa catgcccagt ccggtcacaa ctacacgacg
     9301 cttagacacg tttgtcctcc agggagggaa aaaatgattc tagtgggaca aaaagataaa
     9361 actcaggcgg tcgaacgacc gcctggagat gttcacttac gcctggtggc cgttgatgta
     9421 atcaatggca gcctgaacgg tggtgatttt ctcagcttct tcgtccggaa tctcagtatc
     9481 aaactcttct tccagagcca ttaccagctc aacggtgtca agagaatccg cgcccaggtc
     9541 ttcaacgaaa gaagcattgt tggtaacttc ttcctgctta acgcccagct gttcgccgat
     9601 aattttctta acgcgttctt cgatagtgct catactctta aatttcctat caaaactcgc
     9661 tttcgcgatg gttttcgtag tgtataaaat gttgaaaaat ttgcaactaa atcccggcag
     9721 gtcttaccac gattttacgt tattttgagg ccttaccccc caataacgca aatcattttg
     9781 cactaattat tactcataac cacgcagact gcgcgcaaca tgagcatttt gtgcaaatcg
     9841 cggtcagacc atgtacatcc cgccgttcac atgcaaagtt tcacccgtga tgtaagctgc
     9901 ttcgtcggat gccaggaatg caaccgcgtt ggcgatttcc tgtgcgccgc cgaggcgacc
     9961 cgcaggaacc tgcgccagga tacccgcacg ctggtcatcg ctcagcgcac gtgtcatgtc
    10021 cgtttcaata aagcccggag caacaacgtt tacagtaata ccgcgtgacg caacttcgcg
    10081 cgccagtgat ttactgaagc cgatcaagcc cgctttcgcc gcagcgtagt tggcctgacc
    10141 gccatttccc atggtaccaa ccacagaacc gatagtgata atacgaccat gacgcttttt
    10201 catcatagcg cgcattaccg cttttgacag acggaaaaca gatgaaaggt tggtttcgat
    10261 aatatcgttc cactcttcat ctttcattcg cattaacagg ttatcacgag tgataccggc
    10321 attattgacc aggatatcca cttcaccaaa ttctgcgcga attttttcca gaacagattc
    10381 gatagatgcc gggtcggtca cattcaacat cagacctttg ccgttagcac ctaaataatc
    10441 actgatcgcc tgagcgccat tttcactggt cgcagtgcca ataactttcg cgccacgggc
    10501 tgcgagcgtt tcagcaattg cgcggccaat tccgcggctt gcaccggtta ccagtgcgat
    10561 ttttccttca aaattcatga ttttcctctt ttaaagctcg agcgccgctg ccatcgctga
    10621 aggttcgttc agcgccgagg cggtcagggt gtcgacaatg cgtttcgtca ggccagttag
    10681 cactttgccc gggccgactt catagagatg ttctacgcct tgcgctgcca tgtactcaac
    10741 agacttcgtc cactgaaccg ggttatacaa ctgacgtacc agtgcgtcac gaatggcatc
    10801 accattggtt tcgcatttca catcaacgtt attcacaaca ggaactgttg gtgcgttaaa
    10861 ggtgattttc gctaattcta ctgccagttt gtcggctgct ggtttcatca gcgcacagtg
    10921 agacggtacg ctcactggta acggcagcgc gcgttttgcg cccgcagctt tacaggcagc
    10981 gccagcacgc tcaaccgcct ctttatgacc ggcaataacc acctgtcccg gagagttaaa
    11041 gtttaccgga gaaacgacct gaccttctgc agcttcttca cacgctttcg caatagacgc
    11101 atcatccaga ccgatgattg ccgccatagc gcccgtgcct tctggtacgg cttcttgcat
    11161 gaacttgccg cgcatctcaa ccagacgcac cgcatcagcg aaatcaatca cgccagcgca
    11221 aaccaacgcg gagtattccc ccaggctgtg accggccatc attgccggtg ctttaccgcc
    11281 ctgctgctgc catacgcgat acagcgcaac agatgcagtc aacagcgctg gctgggtttg
    11341 ccaggtttta ttcagttctt cagctggccc ctgctgggtc agcgcccaca ggtcgtagcc
    11401 cagcgccgca gaagcttcag caaacgtttc ttcgacaatt ggatagctcg ccgccatatc
    11461 agccagcatt ccaacggttt gagaaccctg tccagggaac acaaatgcaa attgcgtcat
    11521 gttttaatcc ttatcctaga aacgaaccag cgcggagccc caggtgaatc caccgccaaa
    11581 ggcttcaagc agaaccaact gccccggctt aatgcgcccg tcgcgtacag cttcatccag
    11641 cgcgcacggg acagaggccg cagaggtatt accgtggcga tccaacgtca ccacgacatt
    11701 gtccatagac ataccgagtt ttttcgccgt tgcactgata atacgcaggt tagcctgatg
    11761 cggaaccagc cagtccagtt gagaacggtc aagattattc gccgccagcg tctcatcaac
    11821 gatgtgcgcc agttccgtta ccgcaacctt gaagacttcg ttgcccgcca tcgtcagatg
    11881 aattgaattc tctggattca cgcggtcggc gtttggcagc gtcagcaatt caccataact
    11941 accgtcggca tgcagatggg tggagatgat tcccggctct tcagaggcag ccagcaccgc
    12001 agcgcccgcg ccatcgccaa aaataataat agtcccacga tctgttggat cgcaggtgcg
    12061 cgccagtaca tcggaaccga cgaccagagc atacttcacc gccccagatt tcacgtattg
    12121 attggctacg cttaatgcat aggtgaaacc tgcgcaggct gctgcaacgt caaatgccgg
    12181 gcaaccttta atgcccaaca tgctttgaat ctgacaagct gcgctcggga aagcgtgcgt
    12241 agcagaagtc gttgccacaa cgatcaggcc aatctggtct ttctcaatgc ccgccatctc
    12301 aattgcgcgt gtcgccgctt caaagcccat ggttgaaacg gtttcgtttg gcgcggcaat
    12361 gtggcgttcg cggataccgg tacgagtgac aatccactcg tcagaggtgt ccaccatttt
    12421 ttccaaatcg gcgtttgtcc gcacttgttc gggcagatag ctgccagtac caataatctt
    12481 cgtatacatg tacgctcagt cacttttcgg ttatataccg tcacttgcaa actgcgagtt
    12541 cgctggcagc gtcctgctac cgcagagttc cgcttttgcc accgtccagc agctcaaaac
    12601 cagctgggta tacagattcc aggcgagcgg caattcgctg aggaacttgt cgctgcaccg
    12661 cctgcactgc ctgttcaatc gcgaccgcaa aagctcgctg attggctgca ccatgacttt
    12721 ttatcaccgt gccgcgcaat cctaacagac aggcgccgtt atactggtcg gggttgaggt
    12781 gactgaatcg cctcgtcagg ctcttttgta gccaacgctt taataacagt agccaccacg
    12841 accgtttttt cccttcaccc tgagatttca gcagagaaag gaacatcctg acaacacctt
    12901 ccatcgtctt taacgtgaca tttcctgtaa agccgtcaca aaccagcaca tctgtcttgc
    12961 cagttaacaa ctcattggct tcaagatagc cgatataatt gatagaaggg attgttttaa
    13021 gcaccgctga ggcatcccga atactgtcga gaccctttac ttcttcttca ccaatattga
    13081 gcaacgccac gcgaggattg ggaatttcca ccacctcttc agccagaact gagcccataa
    13141 tggcaaattg caccaacatt gtgctgtcac aatcgacgtt ggcccctaaa tcgaggacca
    13201 ccgttttgcc cttttgctga tgtggtaata ccgtcaccag cgccggacgc tcaatcccct
    13261 ccaggggcct gagtaataat tttgccagcc ccatcagtgc cccggtatta ccggcactga
    13321 cacaggcttg cgctcgacct tctttcacca gctccagggc catacgcatt gaactcccac
    13381 gactagcgcg gatagcttgc gaaggccggg catcactggc gataactgac tgcgcaggaa
    13441 taatctgcag acgcgaacgt tgttcaaagt cagctttagc aagtaatggc gtgatggcgt
    13501 cgggattgcc gactaaaaga agagtgagtt gcgaattaga attcagtgcc tgcaatgctg
    13561 caggcactgt cacggaaggg ccaaaatccc ctcccatgac atctaacgcc agggttagac
    13621 gtgtcaaggt atcgtcgccg cccggtttgg tctttcccca gttgcctggg gaaaatcctc
    13681 actaagcttc atcacgcaga cgcgtgatta cttagcgatg accttgcggc cgcggtagta
    13741 accgtcggca gtgatatggt gacgcaggtg tttttcacca gaagttttgt ctacagacag
    13801 gctggtgact gcggtcagcg cgtcatggga acgacgcatg ccacgtttgg aacgggttgg
    13861 tttattctgt tgtacggcca tggaccttac tcctcaatta cggtttatcc ccgatccaac
    13921 ggggagcacc aattacttac gctttaagct ggctaatacg gcaaatgggt ttggcttttg
    13981 cacttcttca ggcagttcac caaagaccat gtccgcttcg gacacttcac agtgttcaga
    14041 atcatgcacc ggaactaccg gcaaggcgag gatgatttca tcttcaacca ttgcaagcag
    14101 atcgatttca ccgaattcgt taacctcaat cggttcatac gcttccggca gtgcttcagc
    14161 ctgttcgtct gaacgcacag gactaaaaca atacgttgtg tagacctgat gagtaaacgg
    14221 cttcccgcaa cgctgacact cgagcgttac cgtcaccttc gcatcgccgt ttaacactgc
    14281 gagacgttgg ttatcgatag cgaacgacat ggagcattcc acatcactgt ccacactgac
    14341 tacggattcg gcgacgcgct caacctgatc aggggtatag ataccctggt aatcaaggcg
    14401 tttttgagcc gtacgaaccg gatcgagagt caggggtaat tttacctttt gcatagggcg
    14461 cgcatattaa ctttgtaacg tcatagagtc aaagaaaaag gcagcctgtg gttgcctttt
    14521 gccaataatt cgcacacatt gcgggttact gctttatttt cgttcagtgc tgataacgaa
    14581 catcaacctg caaaacgaag cgcatagttt aaaatggcta tcatcaatac gctatatctg
    14641 atggaaaaaa atatgcctaa acttatttta gcctccacct cgccatggcg tcgcgctctt
    14701 ctggaaaaac tgcaaatctc ttttgaatgt gcagcaccag aggtcgacga aaccccacgc
    14761 agcgacgaat caccgcgaca gttggtgctt cgactggcac aagaaaaagc gcaatctctg
    14821 gcgtcaggtt atccggatca tttaattatt ggttcagacc aggtatgtgt tcttgatggc
    14881 gaaatcaccg gtaaaccgtt aacggaagaa aatgcccgtc tgcaattacg caaagccagc
    14941 ggcaatatcg tcaccttcta tactggactg gcgttgttta attcggcgaa tgggcatcta
    15001 caaacagaag tggagccttt tgacgtccat ttccgtcacc tgtgcgaggc ggagattgat
    15061 aattacgtgc gtaaggagca tcccctgcac tgcgcgggta gctttaagag tgaaggattt
    15121 ggcattacgc tgtttgagcg tttagaggga cgtgacccta acacgctggt tggtttaccg
    15181 ctaatcgcgc tgtgtcagat gttacgtcgg gaagggaaaa acccgctgat gggatagttg
    15241 tttgcgccgg atgcggttac gtcgcatccg gcaatcggtg caggcggcct gatgcggcgt
    15301 aaacgcctta tcaggccaac cgcccattat caggcttata tgcttgtatt agcgcgcgtt
    15361 acgcagcttt tgcaaacaac gcttcaaacc ttcatccatc ggcgcttcga tacgcatcac
    15421 ctcaccggtc cccggatggg taaacttcaa cgctgcggcg tgcaggaaca gacgatttaa
    15481 tcccgtgcct gcttcagtga gctgtctgtc aaattcacgg tcaccgtagc gatcgtcaaa
    15541 ggcaatcgga tgacccgcat actgtgtatg cacacggatc tgatgagtac gtccggttac
    15601 cgggctacaa cgcaccaggg tggcaaatgc atagcgttct tccactttaa agcgtgtttc
    15661 cgacggtttg ccttcctgac tgacacgcac gatacgttcg ccgctttgca gaatattttt
    15721 caataacggc gcttgaacgc tcttcacatg cgactgccac tgactgcgca ccagcgccag
    15781 gtaatctttt tgcattcctt tttcacgtaa ttgctcatgc agagaacgca acgccgagcg
    15841 ttttttcgct accagcaaca cacctgaagt gtcccggtcg agacgatgaa ccagttcaag
    15901 gaaccgcgct tccgggcgca acgcccgcaa accttcgata acgccgaagc ttaaaccact
    15961 gccgccatgt accgccgtac cggaaggttt attcagcacc aggatatgat catcttcata
    16021 taagatgacg tccgccagcg ccgccacctt ttgcagatgt ggcgaaaccg cctcttcttc
    16081 ccgctcagca acgcgaaccg gtgggatacg cacctcatca cccgcttcga gtttatattc
    16141 aggcttaata cgttttttgt tcacccgcac ttcgccttta cgcaaaatac ggtaaatcat
    16201 actttttggt acgcctttca attgggtacg caaaaagttg tcgatacgtt gccccgcttc
    16261 gtcagcggtg atagcaacaa tttttacgga tggagtctct gttttcatgg tgggcgattc
    16321 taattagcca acaggattcg cgccactcat ttttctatgc ttatatttag ttttacacct
    16381 tattacttca ctacgtgatc actttattga tggttattaa accaatcacc agcaagaagt
    16441 gaaaaatgta atgaacgcat cccacacaat taaacacgga gcagtatgat ggttaaaagg
    16501 gtgaaactgg caggtgtgga gcctcgcaga tttccggcat catagtgccc aatacgggat
    16561 gcataaagct ccctcagact ggagactcca caaatgaaat atacaccggt tggcgttgat
    16621 atcgcaaaac atgtcattca gattcacttc atcaatgagc acacaggtga agtggttgat
    16681 aaacagttgc gtagacagga ttttctgacg ttcttcggca accgtgagcc atgcctgatt
    16741 ggtatggagg cctgtggagg ttctcagcac tgggcacggg aactgacaaa acttggtcat
    16801 aaagtccggt tgttgcaggc ccgcttcgtt aaggcattcg tcatgggcaa taagaatgat
    16861 gtgatggatg cccgggctat ctggatggcg gttcagcagc cgggtaaaga aatcgccgta
    16921 aaaacagaag aacagcagtc ggtactggtt ctgcaccgta cccgcatgca actggtgaag
    16981 ttccggaccg cacaaattaa tgccctgcac gggacgttac tggagtttgg tgaaaccatc
    17041 cacaaaggcc gggcagcgat ggagcgggag ttccccgaag cactggaacg gatgaaagag
    17101 agactgccac cgtatctcat tatggttctg gaaaaccagt acaaccgact gaatgagctg
    17161 gactcactga tagaggatat tgaaaaacag cttaccagcg tggcgaggca gaatgaaacc
    17221 tgtaagcggt tgctggatat tcctggcgtt ggaccactta ttgcgacggc agcggtggcc
    17281 accatggggg aagcatcagc gtttaaatcg gggcgagagt tcgccgcata tgttggtctg
    17341 gttccaaaac aaactggctc cggagggaaa gtacgtctgc tggggataag caaacgtggt
    17401 gacacttatc tcaggacatt atttatccac ggtgcaagag cggtggcatt agtagctaaa
    17461 gagcctggcc cgtggataac cgaactgaaa aaacgtcgtc cagccagtgt ggcaatcgtc
    17521 gccatggcaa acaagctggc acgaacagta tgggcgataa ccgcccatga ccgtaagtat
    17581 gacaggaacc acgtcagtat cagaccatat taatcgctga taccattaaa caatgaactc
    17641 ttaacaaaag ggtgaatgct gaaaggttgc tatggcggcc agagtgatga caaagacagg
    17701 taagaccgtg actcactaaa cctgaacagt attttgggct tgaagtccgc cgtgaaaata
    17761 aggggtgagt cggcgaatta cataggggct cgcagcgtta cggctgcaat aaagccggat
    17821 ataaagctgc aacctacccg tcatgtcaaa acaatggatg ccttgcaaac gggatgcgtt
    17881 catataaaaa ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc tataacaagg
    17941 cttgcagtgg aataatgaag ccgtttccgt gtccatcctt gttaaaacaa gaaattttac
    18001 ggaataaccc attttgcccg accgatcatc cacgcagcaa tggcgtaaga cgtattgatc
    18061 tttcaggcag ttagcgggct gcgggttgca gtccttaccg gtagatggaa atatttctgg
    18121 agagtaatac ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg aaaaacgggc
    18181 aaccgacact ctgcgcctct ttgagctgac gataaccgtg aggttggcga cgcgactaga
    18241 cacgaggcca tcggttcaca cccggaaagg cgttactttg cccgcagctt agtcgtcaat
    18301 gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat caacgcaact cagcaggaag
    18361 agttgcgcgt tgcccttgta gatgggcagc gtctgtatga cctggatatc gaaagtccag
    18421 ggcacgagca gaaaaaggca aacatctaca aaggtaaaat cacccgcatt gaaccgagtc
    18481 tggaagctgc ttttgttgat tacggcgctg aacgtcacgg tttcctccca ctaaaagaaa
    18541 ttgcccgcga atatttccct gctaactaca gtgctcatgg tcgtcccaac attaaagatg
    18601 tgttgcgtga aggtcaggaa gtcattgttc agatcgataa agaagagcgc ggcaacaaag
    18661 gcgcggcatt aaccaccttt atcagtctgg cgggtagcta tctggttctg atgccgaaca
    18721 acccgcgcgc gggtggcatt tctcgccgta tcgaaggcga cgaccgtacc gaattaaaag
    18781 aagcactggc aagccttgaa ctgccggaag gcatggggct tatcgtgcgc accgctggcg
    18841 tcggcaaatc tgctgaggcg ctgcaatggg atttaagctt ccgtctgaaa cactgggaag
    18901 ccatcaaaaa agccgctgaa agccgcccgg ccccgttcct gattcatcag gagagcaacg
    18961 taatcgttcg cgctttccgc gattacttac gccaggacat cggcgaaatc cttatcgata
    19021 acccgaaagt gctcgaactg gcacgtcagc atatcgctgc attaggtcgc ccggatttca
    19081 gcagcaaaat caaactgtac accggcgaga tcccgctgtt cagccactac cagatcgagt
    19141 cgcagatcga gtccgccttc cagcgtgaag tccgtctgcc gtccggtggt tcgattgtta
    19201 tcgacagtac cgaagcgtta actgccatcg acatcaactc cgcacgcgcg acccgcggcg
    19261 gcgatatcga agaaaccgcg tttaacacca acctcgaagc tgccgatgag attgctcgtc
    19321 agctgcgcct gcgtgacctc ggcggcctga ttgttatcga cttcatcgac atgacgcctg
    19381 tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag gaccgtgcgc
    19441 gtattcaaat cagccatatt tctcgctttg gcctgctgga aatgtcccgt cagcgcctga
    19501 gcccatcact gggtgaatcc agtcatcacg tttgtccgcg ttgttctggt actggcaccg
    19561 tgcgtgacaa cgaatcgctg tcgctctcta ttctgcgtct gatcgaagaa gaagcgctga
    19621 aagagaacac ccaggaagtt cacgccattg ttcctgtacc aatcgcttct tacctgctga
    19681 atgaaaaacg ttctgcggta aatgccattg aaactcgtca ggacggtgtg cgctgcgtga
    19741 ttgtgccaaa cgatcagatg gaaaccccgc actaccacgt gctgcgcgtg cgtaaagggg
    19801 aagaaacccc aaccttaagc tacatgctgc cgaagctgca tgaagaagcg atggcgctgc
    19861 cgtctgaaga agagttcgct gaacgtaagc gtccggaaca acctgcgctg gcaacctttg
    19921 ccatgccgga tgtgccgcca gcgccaacgc cagctgaacc tgccgcgcct gttgtagctc
    19981 cagcaccgaa agctgcaccg gcaacaccag cagctcctgc acaacctggg ctgttgagcc
    20041 gcttcttcgg cgcactgaaa gcgctgttca gcggtagtga agaaaccaaa ccgaccgagc
    20101 aaccagcacc gaaagcagaa gcgaaaccgg aacgtcaaca ggatcgtcgc aagcctcgtc
    20161 agaacaaccg ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt actgaaggca
    20221 gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcag actgccgaga
    20281 cgcgtgagag ccgtcagcaa gctgaggtaa cggaaaaagc acgtaccacc gacgagcagc
    20341 aagcgccgcg tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag gcgcaacaag
    20401 aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga aaccgaacag gaagaacgtg
    20461 tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg cgttacgagc
    20521 aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga agaaactgcc gctgccgaac
    20581 caattgttca ggaagctcca gctccacgca cagaactggt gaaagtcccg ctgccagtcg
    20641 tagcgcaaac tgcaccagaa cagcaagaag agaacaatgc tgataaccgt gacaacggtg
    20701 gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt cagcgtcgtc
    20761 gtcgctatcg tgacgagcgt tatccaaccc agtcgccaat gccgttgacc gtagcgtgcg
    20821 cgtctccgga actggcctct ggcaaagtct ggatccgcta tccaattgta cgtccgcaag
    20881 atgtacaagt tgaagagcag cgcgaacagg aagaagtaca agtgcagccg atggtgactg
    20941 aggtccctgt cgccgccgct gtcgaaccgg ttgttagcgc gccagttgtt gaagaaatgg
    21001 ctgaagtcgt agaagccccc gtaccggttg ccgaaccgca accggaagtg gttgaaacga
    21061 cgcatcctga agtgattgcc gccgcggtaa ctgaacagcc gcaggtgatt accgagtctg
    21121 atgttgccgt agcccaggaa gttgcagaac acgcagaacc ggtggttgaa ccgcaggaag
    21181 agacggcaga tattgaagaa gttgccgaaa ctgctgaggt tgtggttgca gaacctgaag
    21241 ttgttgctca acctgccgcg ccagtcgtcg ctgaagtcgc agcagaagtt gaaacggtag
    21301 ccgcggttga accagagatc accgttgagc ataaccacgc taccgcgcca atgacgcgcg
    21361 ctccggcacc ggaatatgtt ccggaggcac cgcatcacag tgactggcag cgccctactt
    21421 ttgccttcga aggtaaaggt gccgcaggtg gtcatacggc aacgcatcat gcctctgccg
    21481 ctcctgcgcg tccgcaacct gttgagtaat aattagctca aagtaatcaa gccctggtaa
    21541 ctgccagggc ttttttattt catgtttgaa tcaacatatt aaaattcatc aaatggcatc
    21601 cttgctaacc aacaatgcaa aataggcaaa aaaaatgccc gccccggcga tccctgagcg
    21661 ggcagacaaa acatacccag tttcatgata tgttttaaag cgagcttatt tgttgagctg
    21721 gaagagcgac aatccctgca tatcggtaaa tgctttatac gatgcctgca atgccgtttg
    21781 ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat cgctcatctg
    21841 ctgcgtttgc cctaaagcgc gatcgctacc taaggaatcc agcgactcca gttcgttcag
    21901 ctgcgtgcct aattccgcgc gaacggcaag gacattattc agcgagtttt tcagtccacg
    21961 gttggtttta tccaatgccg cagcggcgat ttctttatcc gcttcgctat ccgcgaccgg
    22021 cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt cagaagcgct
    22081 accgtctggc tccgcaactg cgttgctggt aatactgtcg aaaattttgt cacccgtgtg
    22141 ccctatcacc atcgaacgcg aagcatcgac ctgttgctta atactttctg ctccaccgac
    22201 gtatttccct ttctcttcgc taaacggcgc agtctctgtt ttataaccgg caaaaatgta
    22261 gcgcccgtta ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa gcccctgaat
    22321 atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg cgtagacaat
    22381 tttttcctga gcattctgga tagcagtggt gacctggcta agtacactct cttccagtga
    22441 cactttttga atggcgaaag tacgcgccag cgtgtactgg ctgttttgcg cctgtgcctg
    22501 ggagagaact acggcttgtg atgctgcaat gggatcgtca gaagggttaa cgactcgctt
    22561 acctgtcgac atctgctcgc cgtacttcat ccattccgcc tgagaattgg tgatgccacg
    22621 catgttttgc tggtacatca tctgggtact gaaacgcatc ttatctcccc ttagcgaatg
    22681 ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt cgccaggtaa
    22741 tactgctgaa aacgttgcag atttccgtac tcttcgtcga gattgacgcc agagatagac
    22801 tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg tggcgctact ggttttcaac
    22861 gtcgcggttt tattaccgat atcactcact aacgaggcat aagcgtcgtt aaaggatttc
    22921 gcaccgccca ccgttttact gttgctttgc agatccagca gggcctgacc gttgcggtta
    22981 tcgctatcac ccgcatcttt ttcgctcgcc atcgctattt tcgcttcgtc ggtgattaat
    23041 acatccatgt tgacgatggc gtcacttact ggtttcagcg tgaagctgtc gttaacggca
    23101 ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt ggcatccggc
    23161 gtcaccgtaa aagtggtatt gctggcaagg cgggtgacct gccactgatt attatcgaac
    23221 gagattttgt aatctgtcgc cagtaccgcg gaggcatcag ttaccgtggc accgatcgca
    23281 acgtcacctt tgtttttcgt gttttgcaaa accgcgggct taccgatagc aaagaaatct
    23341 tcaccagcat cgccgttggc atcaaatccg gctttgtgtt gggtgttgaa agcctcggca
    23401 aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt ccagatcctg agaacggaat
    23461 gtcagaatgc cgcccagcga cccggtattc agtaatttct ccgggatctc aatattgcct
    23521 gccgtcccat caacgtaagc gacagtcgta cgagaagggt cggcgctgga aggaactgcc
    23581 gccagttgcc gcgccgtact tccctgaacc agtgagtaac cattggccat cgtgatgtta
    23641 taagtgccgc catcctgaac gctgacttct acaccaacaa tctggtttaa ttcgctcacc
    23701 agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac gcctgtcagg
    23761 cgggagattt ggtcgttcag gctggcgatt tgtttagcgt aattgttgat ctgatcaacg
    23821 ctggcaccta tcgcgatatt gacctgtttg tcctggtcac gaagatactg atcggtggtt
    23881 ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc tgccgggtct
    23941 tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg tgttgccagc
    24001 gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata gcgggcagtc
    24061 aggccgctac tttgcgtctg cgccgcacgt aactggttgg tgataaacgc atcatactca
    24121 cgctgcacac cagaaacgta gacaccattg ccaacccagc cgccagcgcc caacgtgcta
    24181 ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata gctggagata
    24241 ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat ggcgttatta
    24301 atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta tcggcagcga
    24361 cccgccggac ttgagttatt caaaacagat tatcaatgtt catactgtag gttttgctca
    24421 ccttgtcgct tatcgatttc atctgctgaa tcatgttggt gagtttgcgg gcatagtgag
    24481 gatcggtggc atagcccgcg tcctgtaggg cctgcgcccc ctgctccgca ctcgtggcgg
    24541 tggtcacggc ggcgtagcgc gggttacgcg ttaacagccc aacgtaatcc gactatgctt
    24601 ccagatacga gctgtagacc cgaaacttcg cttttacttt cttcgcttcg ccgttttcat
    24661 attcagtcgt ggtgatttca gtaactggcc ctttccagtt gccagaggct ttgacaccaa
    24721 acaggttata gctcggctcg ccgttttcgc ggcggatttg ccgttgcccc caaccagatt
    24781 ccagcgccgc ctgagcgagg atcaaatgat gtggcacacc gctttgctgg cttgccagtt
    24841 gggcgggcag cgagagttgc gcgaggaatg ctttactgtc acccggcagc gaatcatcgt
    24901 agttacgtgg cacggccttt tgcaccagct gcgaaagcgc ctgattttga taacgcacca
    24961 cagtttcgag cgggaatttc atcggtgctg ctggcgtgga ctcctctggc aatggttgtt
    25021 ctggcgtcat ctgtttaacc atcatctctg caagccccag acctttgccc gccgtcatct
    25081 gttgggcaat ctgctggtca tacatactgg tatacaggcg agtgtgctcg ctgctgaaca
    25141 ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg aacatccctt
    25201 ccacctgacg ggccaccgga cggatatttg ccgccggatc ttcgcccgct ttcgccttta
    25261 gttcgttgag tgattgcgca tcccaggccg cacttgccag tagtttgcta tcgctgatca
    25321 tcagatgatt tccagttttg cccgcagaca tcccgcactt tgcattgatt gcagtatgga
    25381 catcagatcc atcggcgtag cgcccagcgc attgagcgcg cgcaccacgt tattgagcct
    25441 ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg cgtaaatcga tctgcgtttg
    25501 tggagtaacc acagtctgtc caccaccaaa cggtgtatct ggctggctga cattggcctg
    25561 acgattaact gttactgaga gattcccctg cgctaccgcg cagctgtcga gggtcacttc
    25621 gcgattcatc accaccgaac cggtgcgcga gttaatcact actttagcgt cctgcggggt
    25681 gacattaacc tgcatattct ggatatcggc aaggaagcgg acctgggaac tgttgccact
    25741 cggtacgcgc acctgaatag tccgcgcatc taacgcggtg gcgctgccat atccacgcac
    25801 gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa tcttcgtcgt taagttgcaa
    25861 attaagggta ttcccgacgc caaactggct gggcaattca cgttcaataa ccgcaccatt
    25921 ggtgatccgt ccaccgttca gttggttaac ctgaacactg ctaccgccag cggaggctcc
    25981 tgcgccgcta accagaatat tgccctgcgc cagcgcatac acctgactgt caacgccctt
    26041 aagcggtgtc atcaacaacg tacctccacg caagcttttg gcattaccca tggaagaaac
    26101 caccacatcg atggtttgcc cctgacgtcc aaacggagga agtgacgctg tcaccattac
    26161 cgcagcgacg ttttttagct gcatattggt gcccgtcgga acggtaattc ccagctgtga
    26221 gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg gttgtctggt caccggtgcc
    26281 atccagcccc accaccagac catagccaat cagtgagttt tgccttaccc cctgaacact
    26341 ggtgagatcg cgaatacgct cagcctgagc cgccgtcgtg accagtagaa gaattaatgc
    26401 agagagaaat ttaatcacca cagcctcact tacattggcg acaggttaag gaagaaacgc
    26461 tgcaaccagc ccatattttg cgcttcgtta atgtagccat tgcctacgta ttcaatgcgc
    26521 gcatccacca cctgagtaga cggtacggta ttgctgccgc tgatagtgcg tggattaacc
    26581 acgccagaga agcgaataaa ttcggtaccc tgattaatgg caatctgttt ttcacccacc
    26641 acatgcaggt tgccgttgac cagtacctgg tcaaccgtca ccgtcaacgt gccgctaaag
    26701 gtattgctgg cattggcccc gccctttccg ttgaacgtgt taccaccgga ggcttcgaca
    26761 tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg gcacagtatc aaagccaaaa
    26821 ttagttttac cgtcacggct ggcattcgca gaggagcttt tgctggcgct gacgttctcc
    26881 tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc aaacagcggt
    26941 tgatagccat agttaatcgg ctgagcagac tggaaaatag aaccgttggc gacgggcgtc
    27001 ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca gcggcgtgga gggtatccag
    27061 gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg cataagtatg cgcagcgttt
    27121 ttttgcatgg ctatcatctt caaaaatcag taaaccggtg aacctgccac cggttaagcc
    27181 ttagagttgc gtcagttttt gctgcatctg atcggtggtg gacaccgctt tactgttgat
    27241 ttcgtaagcg cgttgcacct gaatcatatt gaccagttct tccgccacgt tgacgttaga
    27301 cgtttcaaca tacccttgat acagcaatcc cgcgccgttc aggcccggcg tgctttcgtt
    27361 cggtgcacca gaggattgcg tttcggtgta gaggttttcg ccaatgctct ccagcccggt
    27421 gtcattcata aaggtggtga gattgagctg cccaacctga accggagctg cctggccttg
    27481 ttgggttacg ctgaccacgc catcacgacc gatggtgata cttaacgcat tcgccggaat
    27541 ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc agctgcccgt tctgatccac
    27601 ctggaaagag ccgtcacggg tataggctga tgaaccatct ggcaacatca cctggaaaaa
    27661 gccctgccct ttaatcgcga catctttgct gttgttggtc tgcgacagat ttccctggct
    27721 gtgtaagcgt tcagtggcga ccgggcgtac ccccgtgccg atttgtaatc cggagggtaa
    27781 ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg gtttgataaa gcagatcttc
    27841 aaacaccgcg cgctgacgct taaaaccgtt agtactgacg tttgccaggt tgttggcaat
    27901 gacgtccata ttggtttgct gggcgtcaag gcccgttttg gcgatccata atgaactgat
    27961 cataaaatgt cctgtagtgg attagtgaca agcagagcag aagcacgcga cagtgtgaat
    28021 tccccctcac tctaaccctc tccccaaagg ggcgagggaa tagtccgcgc gcttttattg
    28081 caatgtcatc aatcgttcaa ttaactcatc gacagcagtt ggttggcacg gcctgcgtta
    28141 tcatcgacgc tgctgatcac cttcatctgc atttcaaaac gccgcgcgct ggcaatcatg
    28201 tcgctcattg ccgcaacggc attgacgtta ctgccttcca gaacccccga catcacacgc
    28261 aaggttggat ctgcctgcag taccggccct cgcgtggcct gggtttctgc gcttaaacga
    28321 aaaatgccgt cgtcaccgcg ctgcacttcg ctgcccgtgg ctttcaccag tttcagacgc
    28381 cctactggcg caactgtatt tgccggatcg cccggattga gcgccgagat tgtgccatcg
    28441 gcagcaatag tgatttccgc tccttccggc acagcaattg gccccgcctc gcctatcacc
    28501 tgatgcccct gaattgtcag ttgcccggtg ggatcaacct gaatgctgcc attacgcgta
    28561 tacccttcgc tgccgtcagc ggtctgcacg gccagccagc catcctgctg caacgcaacg
    28621 tccagcgggc gcgaggtgta atctatcttg ccgggcgtca tgtctgcacc cggtgttgac
    28681 gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc gcgcaaggcg
    28741 tttaactgcg cgcgaaaacc gggcgtagag gcattggcca gattactggc ggttaccgcc
    28801 tgttgattca gcgtctggct ggctgctccc atcgcagtat aaattgcgtg atccattgag
    28861 ctatcccgtc agcgattagc gtaagttaac cagcgtgttg aggatctggt cctgggtttt
    28921 gatggtctgg gcgttagact gatagttacg ctgggcaacg atcatattga ccagttcttt
    28981 actgagatcg acgttggacg cttccagcgc accgttggtc agggtgccaa agtttcccgt
    29041 cccggctgtc cccaacagcg ccacgccaga agattgcgtc gcagaccaga cgttgtcgcc
    29101 ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt acaatctgcc ccagcagttg
    29161 agtttgttcg ttggaatagt tgccgacaac cgtaccgtca tcattgattt gataactcac
    29221 cagatcgccc ggtttgtagc cgttctgggg ggttgccaca atattgttag cgccggtatt
    29281 ttgctgcatg gagttgagga agctcagact aaacgtggcg ggttctgcac cgttaattgc
    29341 gccggttgcg atattattcg ccatcgcacc atccactaat gtgccattag cattaaattc
    29401 cagtgttgtc gctgtcttcg caatgctgtt tggatcactg ctatcctggg tgtagacctg
    29461 ccagttatta tccccggtct tcacaaagta gacgctcatg tcatgagcat taccctgact
    29521 gtcgaaaaca gtcaccgaac cttttttgtt atagctatcc gcattgctgg cgctgaatgg
    29581 cgtaacagta ggaagcggat cactggaatt caggttgatc tgcatcgacg ccgtggtggt
    29641 agttttcgct gccatcaggg tattcgggat cgaaatattg gtcggattcg ccccttgctg
    29701 aatagtcggc ggcgtaccgg ttgccgggta acccgtcagc tgtaaacctt gcatattcac
    29761 caggttacgg ttttcatcca gcttaaattg tccgttacgg ctgtagaaca ccgaaccgtt
    29821 gctgtctacc agacggaaaa aaccgttctg gctgatagca acgtccagac ctcgcccggt
    29881 gttggtggtc gtgccatctg taaagtcctg agtgataccg gcaactttta cccccagtcc
    29941 cactttcgaa ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa agccgtaggt
    30001 ggcggagttg gcaatattgt tgccaataac atcgaggtta gtggcggcag cgtttaatcc
    30061 gctaaccgct tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt aaattatctg
    30121 ccgtacttcg tcgagggtgg tggtgccgta agtgccgaga tccagcgtat taccgctgtt
    30181 gccgcggatc acaccctgca ccagagcaaa ctgcagcggc tgggcaacca gttgtgtacc
    30241 accgttactg gcggtaatcg ctacattgta agaaccgttc ggcgcagtgc tgccatcagt
    30301 caacgtaccg tcccaggtga aactgtgaac tccggcggtc agttcaccaa tatcaatggt
    30361 gcgcacaacc gcgccatttt tatcggtgat ggtggccgta actttgtctg ccgcctgttg
    30421 cagctcaaca ccaaacggcg tggtcgtggt cacagcccct tcttcactgc cggttcccgc
    30481 aagaacagtt gtgccgggga tcatcacgcc gtgaccgatc aggttactgg cctgtaacga
    30541 ctggctgtta tcaatctgtc cggaaataga tccgagcatg gtattgagtt tttcaatccc
    30601 gctgaccgtg ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca ttggattggt
    30661 cgggtcctgg tttttcagct gcgccaccag caaagtcaga aaactgcttt gtaaatctgc
    30721 ggcgttgctg cccgtgagcg aactactgct ggtggtactg acgccggtat ttgtcggatc
    30781 ggtggtggtt accgcaatgg acatagcttt ctcctttatt gaccgagcgt aagggttttc
    30841 agcatcatgc ttttcaccgt gttgagcact tcaacattgg cctgatagct gcgtgacgcc
    30901 gacatggtgt taaccatctc tccgacaaca tcaacgttcg gcatttttac gtagcccttt
    30961 gcatccgcca gcggattacc cggttcataa accagtttgt ccggggcctg actttctata
    31021 acatcggcaa cctttacgcc gcctgtcgca gcacctggtg cagcgttaac ctggaatacc
    31081 acctgttttg cccgatatgg ctgtccatcg ggaccggtca cgctatcagc attcgccaga
    31141 ttactggccg ccacgttcag gcgctgggac tgggcagtta acgccgaccc ggcgatatca
    31201 aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat catgcctttg
    31261 atttgcccgc tcaacgcact aaggctcatc tggtattgca gttctgcggt aggaggcgtc
    31321 agcgcctgcg ccggaatgtg ttgcgttgag gtcatcgtca gagcaaccac actggttgca
    31381 tcccgtccac gttgcatgac ttttttaagt tcactggcaa aatcgatatc gcgcgcctga
    31441 taaccaaggg tatcggcatt ggcgatgttt gctgccagca cttcctgacg ctgggcgcgc
    31501 agattgagcg cctcttgttg aaaacgtaag gcggcgtcga gcttatcgag catatctcct
    31561 ccgcaggtat caaaattctg ccatcagctt aaatgccttt acgcgcgcgt tatcggcgga
    31621 ataaacgcaa aatgggtcgc tatttatgcc gttgatgacc attggggacg ggtacaattc
    31681 acgttgtaga aatagctggg gggcgaaaat gctggcaata aaacgtagcg tggcgatcat
    31741 cgcgatactg ttcagtccgt taagtgcggc gagcaatctc acatcgcaat tgcacacctt
    31801 ttttagcgcc cagctcgtgg gggtaagtga tgaggttcgt gtttctattc gtacagcacc
    31861 caatctacta ccgccgtgcg agcagccatt gctttcgatg agcaataatt cccgcctgtg
    31921 gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa cgatatttac aggttaatgt
    31981 gcaggccaca ggaaattatg tggttgccgc gatgcccatt gtgcggggag gaaagctgga
    32041 agctggcaat gtcaaactaa aacgcggacg actggatacc ctgccaccgc gtacggtgct
    32101 ggatatcaat caacttgttg atgccattag cctacgcgat ctatcacccg atcaaccaat
    32161 ccaattaacc cattttcgcc aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat
    32221 cgccagcggt gacgggttta gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt
    32281 cgcacagaat gcgcggatgc gcatggtatc gggactggtg gtcagcggcg ttgttgatgc
    32341 agatgggaat attcttataa acctgtaagc tgttaaagat tacccgtccc ttgccgataa
    32401 ataagcaaca catgataaaa gcgccctcaa tgaggaataa accatgagta ttgatcgcac
    32461 ttcgcctctg aatcccgtaa gcaccgttca accgcgcgaa accactgacg cgccggtaac
    32521 gaacacccgg gcggcaaaaa caaccgactc caccagcacc agcaccagca ccagcaccag
    32581 caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag
    32641 tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa
    32701 aatggacatc ggcaaaattg ccgatgcgct gatcaacgaa gctcagcaag acttgcagag
    32761 taactgatcg tatgacacgt cttgcagaga tccttgacca gatgtccgct gtgcttaacg
    32821 atctcaaaac ggtaatggat caagagcagc aacatctctc tatggggcag atcaacggca
    32881 gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg gattacctcg
    32941 aacagttacg caagaaagaa cccaatacag caaatagcgt tgatattagt caacgctggc
    33001 aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggttggttac
    33061 tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac
    33121 cgacgctata tggggcgaac ggtcagacct caacaaccca tcgcggcggt aaaaagattt
    33181 cgatctgaag attatctccg gcctgcactg caggccggaa tgcattgtta caccgtccgg
    33241 cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta cgcggcaatc
    33301 cccgccagca cgaccgccat taaacgcagt aagcgccagg gcatggtacc caatgaccac
    33361 tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc
    33421 agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg cttacgcaac
    33481 tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag tgacagcccg
    33541 gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg
    33601 atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg agccaacact
    33661 ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc
    33721 agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa cggacctgaa
    33781 agaatgccca acgcaaccgc actcggcagc gccaacagga aacaaagacg caacccccag
    33841 tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga cagcgacggc
    33901 agcaaaatgg tgccaagcgc cacgcccagc acaccggacg gaaactccat taagcggtcg
    33961 gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg
    34021 attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg tttcaccacg
    34081 cgcattgctc cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc
    34141 aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc
    34201 gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg
    34261 ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct
    34321 cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt tagtagctgg
    34381 ctggtcaggg caaatttgtc agctgtatca gcgaagcctg gcgcggtcac catgatcacc
    34441 cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt cagcaggcca
    34501 gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt atattccgcc
    34561 agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg taacaagtta
    34621 ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg
    34681 acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg
    34741 gccagcgatt ttaataaatt catcggtgtt ctaatccaga ccaacgggca aaacgcctgc
    34801 aagacaggcg ctgaaagagg cgaatagtct acctggatta tggtgaattg ctaccgccag
    34861 atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc
    34921 cagcacggcc tattcgccgg cggtttgcgg aactgtctga ttttgcacac attcaatgaa
    34981 gtgccgcgca cagccgacaa atccacgttg ttcaagagtg ctctgccaac caggaatcgg
    35041 tttatgcact acgccctgcc cgcgctcctc acgccattcg cgcatatccg taatgtcgat
    35101 gagcgcaccg tcagtcacgg cctgcacggt ttcacgctga cttccggcac ggcgatgcat
    35161 acaggtggtg atctgcaaag gaccggccga aaaatggtgc tcggcaaaca gcatttcgcc
    35221 agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg acaaccacag
    35281 cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat cgtgtggccc
    35341 gacactattg ctacgatgtt tatccattct tagcgaagct gcggtagcga gttgcgtttt
    35401 taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg tcagtttttt
    35461 ccgcgccgcc agttccacca gccgttcagc atcgcgcaga ttttctgcca gcggtttatc
    35521 gacacagaca tgtacacccg cattgagtaa cgtactgacc acgtcaaagt ggctggcggt
    35581 gctggaatgc acaaaaaccg catcgcaact ggcggcaagg ctggataacg aatcggcata
    35641 aggaatgcgc cagctttcac aaattggcag ggctttcgcg cgcgtaggcg accaggctcc
    35701 ttgtaacatc cagtcaggcg ctgccgccag caccggtaac cacgcttttt gcgcaatgcc
    35761 gcctaatccc acaacgccga tacgtaattt tttcacttta atctcccaga tgggccagca
    35821 acgaatcaag acgctgtttc agttctgcca cttcgatttc cagggcttcg acgcgggcct
    35881 gtaaatcacc atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc tctacctcac
    35941 cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc agacgcacca
    36001 caaaaggacc atcttcgcga tttgccagtt gttccagcgt cgactccaat tccgccatat
    36061 cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc tgggcaccac
    36121 gcaataacaa cgtggtgatt aacgccactt ccgctgcgct cagtttcaga tcgccaaatt
    36181 ctgaattaca aaaacgttgt tcatatttgg tgacccgatt accaaagcca ctcaccgtgc
    36241 gcagataatg gcgtttgacc agattatcca gttgctcctg cacttcagat tcactcagat
    36301 tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca ttgactgaga
    36361 gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc acgcgagctt
    36421 caagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt ccagtctggg
    36481 gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag atagtctttc
    36541 gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg tttattgtgc
    36601 ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata acgaatggct
    36661 gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat cgaataaccg
    36721 agatagcagg catgaaaaga gccacgaaca acattggaaa aattggcaac gccaataatc
    36781 tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt atgaaattcg
    36841 ttaatcatcc ccagcctggc ctgccagcct gatggataac agtggctttc gtcgcgcact
    36901 ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgcaag acgccaggca
    36961 tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg
    37021 ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt aatacgctgt
    37081 aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc attttctgca
    37141 ttattcacat atttgataaa aactctctat caaagcgacg ttttgataaa aaaatattgt
    37201 ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct catcttttat
    37261 ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa atatttcctg
    37321 ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct gatctctatc
    37381 cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct cggtctacgc
    37441 cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg ctttggtgcc
    37501 aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac aatgggtatc
    37561 gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg cggcacgttg
    37621 tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca acgtggtcgt
    37681 tttttctcgc tgttgatgat gcaggacagt gccggtgcgg tcattggcgc attgttgggg
    37741 agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt tttgtttgtg
    37801 ctgtgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac cgtacgcacg
    37861 cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac ctatgtcctg
    37921 acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc aattatggtc
    37981 aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga agcgtgtctg
    38041 tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaacac
    38101 cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg catggtcagc
    38161 ggcctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat cattgccgag
    38221 cctgcgcgtg aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg cagctatatg
    38281 gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg tggtggctgg
    38341 ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat gctgggaatt
    38401 attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg cgccgcgcgt
    38461 cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca tactgaaagt
    38521 gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac
    38581 tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg
    38641 ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg
    38701 caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta tgtcgataaa
    38761 ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc
    38821 aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat
    38881 gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt
    38941 aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag cgatgatttg
    39001 cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggagaact ttcggaagat
    39061 gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat caactggcca
    39121 agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca
    39181 tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt
    39241 tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt gtcaggatat
    39301 tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat
    39361 cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga
    39421 aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt
    39481 ggctgacgcc catattgttc tgacaaactt gaccagccaa attggtcgcg aagagccgaa
    39541 taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg gtagtcagaa
    39601 agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat
    39661 cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt
    39721 gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta accagcaacc
    39781 tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa aactggcgaa
    39841 aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat gacgagggcg
    39901 cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg
    39961 caaaaacatc ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa
    40021 gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt taaaaactgt
    40081 cgtgccgtat accagcgaaa tttatggacg ggctattgta atgcccaatc tggctccgcc
    40141 cgtgaccact gttgaggctg ccgtggcgta tcgccagcgt attcttgatg ccgtacctgc
    40201 cgggcacaat ttcaccccat tgatgacctg ttatttaaca gattcgctgg atcctaatga
    40261 gctggagcgc ggatttaacg aaggcgtgtt caccgctgca aaactttatc cggcaaacgc
    40321 aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg tacttgagcg
    40381 catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg cagatatcga
    40441 catttttgat cgtgaagcgc actttataga aagcgtgatg gaacctctgc gccagcgcct
    40501 gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg actatgtccg
    40561 tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa
    40621 ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa
    40681 tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag tattcctcgg
    40741 tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct gcgcgggctg
    40801 cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct
    40861 gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatggct tgccggtcaa
    40921 cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac
    40981 tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata
    41041 aaaatcgtag cttcctgttg tcattaggtt attttacctg tataaataac cagtatattc
    41101 aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca ttgccagctg
    41161 gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg tttcctgata
    41221 atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt attggcgtaa
    41281 caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa agcgccgatg
    41341 actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg ccccggcttt
    41401 tttattttac ttacaaattt tgagtacaaa atttctacac ttcctcaaaa taattgcaca
    41461 atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc ttaaaagcag
    41521 aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt ggtatactga
    41581 agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa cacgaattag
    41641 ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt agggtgggac
    41701 atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca gaccccaaat
    41761 aagtccgagc aggaagggac tgaagttggt cagacgctct ggttaaccac tgatgttgcc
    41821 agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg tgatttcccg
    41881 gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg cacctcaccg
    41941 gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg cttaaaccat
    42001 gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg ttccgtaggc
    42061 gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac cgacgcccat
    42121 atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg ccatgcttat
    42181 ggtgaaggcg aaaagtatgt cccgttggtc ctccgcgcgc aaacgctgtg ggatgaactt
    42241 tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa cctcggcccg
    42301 gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca actcaacgtt
    42361 gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt cccggacaac
    42421 tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc gattaaaacc
    42481 tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc ggtcaccgca
    42541 attcgtcatg acgatgatgg cgtaactatt gaaaccgttg acggtgagta tcaggcgaaa
    42601 aaagcgattg tctgcacggg aacatgggta aaagacctgc tcccggagct gcctgtccag
    42661 cctgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt gaagaataaa
    42721 ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt tccggcagaa
    42781 aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc agcggatgaa
    42841 cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt cttgcgcaat
    42901 gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga taattcgcct
    42961 gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct cattaccggc
    43021 ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc cgattttgcg
    43081 caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt ccaataatca
    43141 tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt cggctgttgc
    43201 actatctcat caataatatt cgcgagcatc tgatgcttta tctttttctc tggggattgt
    43261 tggcaattat ggatctgatc tatgtgtttt atttttagtt ttccgcactg acataaactg
    43321 acaaatattt aatctccctt tcatttagcg tgaaaaattg aatgaaatta ttcctgcttc
    43381 tacagcactc accagacatc tctgatgctg aacccactaa acttatgaag attctttcat
    43441 tattaattca aaattattga aataaaaata aatatcattc aatttgttat ttttgtgttg
    43501 caattcattc tccgacacgc cattttcttt ctctcgcaaa ttcgtattgg atgcacttat
    43561 gtcattcaca aatacccctg aacgctatgg tgttatatct gcggccttcc attggctaag
    43621 cgcaattatt gtctacggca tgtttacttt aggtctgtgg atggtcacgc tcagttatta
    43681 cgatggctgg tatcacaaag cacccgaact gcataaaagc atcggtattt tgttaatgat
    43741 ggggctggtt attcgcgttc tgtggcgtgt catatctcca ccgccgggtc cgctgccgag
    43801 ttattcgccc atgactcgtc ttgccgcaaa ggctggacat ctcgcattgt acctattgct
    43861 gttcgccatc ggcatcagcg gctatctgat ctcaactgcc gatggtaaac cgatcagcgt
    43921 ttttggctgg tttgacgtcc ccgcgaccct tgccgacgct ggcgcacagg cagactttgc
    43981 cggtgccctg catttttggc tcgcgtggag cgtcgtagta ctgtccgtta tgcacggatt
    44041 tatggccctg aagcatcatt tcatcgataa agacgacact ctgaagcgca tgctgggaaa
    44101 gtcgtcatct gactatggag tataaaaaat gaaaaaaagc ctgcttggtt taaccttcgc
    44161 gtccctgatg ttctctgccg gttcagcggt tgccgccgat tacaaaattg acaaagaagg
    44221 tcagcacgcc tttgttaatt tccgcatcca gcaccttggc tatagctggt tatacggcac
    44281 ctttaaagat ttcgacggta cttttacctt tgacgaaaaa aatccggctg ccgataaagt
    44341 gaatgtgaca attaacacca ccagcgtcga tactaatcac gccgaacgcg ataaacatct
    44401 tcgcagtgca gatttcctca ataccgcaaa atatccacag gcaacattca cctccaccag
    44461 cgtgaagaaa gacggtgacg aactggatat taccggcgat ctgacgctga atggcgtaac
    44521 caaacccgtc acgctggaag cgaaattaat tggtcagggc aacgacccat ggggtggtaa
    44581 acgtgcaggc ttcgaggccg aaggcaaaat taagctcaag gacttcaata tcaagacaga
    44641 tttaggtcca gcttctcagg aagtggatct gattatttca gtggaaggcg tacagcagaa
    44701 gtaagcgtga aatgttgccg gatggcacgc atccggcaat gatatttatt ctgttggatc
    44761 aggaatgccc agtgttgtat tcagacgtcc acgagactta ttaaagatct tattgccatt
    44821 ttcacgtcct gcccgacggc gtcgctgttc ttctggcggt aacgcgcttt cttcgcagca
    44881 aatctcacta caacaacctt tgtatttttc cgcgcatact ggacactgaa taaacagcag
    44941 gtggcagcca tcatttttac agttggtatg gctgtcgcac ggcgcgccgc actggtggca
    45001 atgcgcgata atctcatccg atatacgttc gcccatccgc tcgtcaaaaa caaaattttt
    45061 gccaataaaa cgcaccggca agccctgctc gcgcgcctta cgggcgtatt caataattcc
    45121 gccctcgata tgccagactt tgttgaatcc gttatgtttc atccaagcac tggccttttc
    45181 acaacgaatg ccgccggtgc agtacatgac gattttttta tctttatgtg cctgcatcat
    45241 ctcgactgct tttggcagct
//
LOCUS       DAJTOR010000008        43882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTOR010000008 DAJTOR010000000
VERSION     DAJTOR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.008"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            86..610
                     /gene="lpfE"
                     /locus_tag="OO249_000451"
     CDS             86..610
                     /gene="lpfE"
                     /locus_tag="OO249_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HCR5634867.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            863..2554
                     /gene="eptB"
                     /locus_tag="OO249_000452"
     CDS             863..2554
                     /gene="eptB"
                     /locus_tag="OO249_000452"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5634868.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            2646..2722
                     /locus_tag="OO249_000453"
     tRNA            2646..2722
                     /locus_tag="OO249_000453"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2680..2682,aa:Pro,seq:cgg)
     gene            3466..5073
                     /gene="dppA"
                     /locus_tag="OO249_000454"
                     /pseudo
     CDS             3466..5073
                     /gene="dppA"
                     /locus_tag="OO249_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
     gene            5381..6400
                     /gene="dppB"
                     /locus_tag="OO249_000455"
     CDS             5381..6400
                     /gene="dppB"
                     /locus_tag="OO249_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5634869.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPASGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            6410..7312
                     /gene="dppC"
                     /locus_tag="OO249_000456"
     CDS             6410..7312
                     /gene="dppC"
                     /locus_tag="OO249_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5634870.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDMLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            7323..8306
                     /gene="dppD"
                     /locus_tag="OO249_000457"
     CDS             7323..8306
                     /gene="dppD"
                     /locus_tag="OO249_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5634871.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            8303..9307
                     /gene="dppF"
                     /locus_tag="OO249_000458"
     CDS             8303..9307
                     /gene="dppF"
                     /locus_tag="OO249_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5634872.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(9337..10608)
                     /locus_tag="OO249_000459"
     CDS             complement(9337..10608)
                     /locus_tag="OO249_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5634873.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            11084..11191
                     /locus_tag="OO249_000460"
     CDS             11084..11191
                     /locus_tag="OO249_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5634874.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(11278..12957)
                     /gene="bcsG"
                     /locus_tag="OO249_000461"
     CDS             complement(11278..12957)
                     /gene="bcsG"
                     /locus_tag="OO249_000461"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5634875.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVETTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTTVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(12954..13145)
                     /gene="bcsF"
                     /locus_tag="OO249_000462"
     CDS             complement(12954..13145)
                     /gene="bcsF"
                     /locus_tag="OO249_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5634876.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(13142..14713)
                     /gene="bcsE"
                     /locus_tag="OO249_000463"
     CDS             complement(13142..14713)
                     /gene="bcsE"
                     /locus_tag="OO249_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5634877.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLTHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            14986..15174
                     /locus_tag="OO249_000464"
     CDS             14986..15174
                     /locus_tag="OO249_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5634878.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            15186..15938
                     /gene="bcsQ"
                     /locus_tag="OO249_000465"
     CDS             15186..15938
                     /gene="bcsQ"
                     /locus_tag="OO249_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5634879.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHRQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            15935..18553
                     /gene="bcsA"
                     /locus_tag="OO249_000466"
     CDS             15935..18553
                     /gene="bcsA"
                     /locus_tag="OO249_000466"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR5634880.1"
                     /translation="MSILTRWLLIPPVNARLIGGYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFILRRP
                     ERSETAQPSDQALAQQ"
     gene            18564..20902
                     /gene="bcsB"
                     /locus_tag="OO249_000467"
                     /pseudo
     CDS             18564..20902
                     /gene="bcsB"
                     /locus_tag="OO249_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            20909..22015
                     /gene="bcsZ"
                     /locus_tag="OO249_000468"
     CDS             20909..22015
                     /gene="bcsZ"
                     /locus_tag="OO249_000468"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5634881.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            21997..25470
                     /gene="bcsC"
                     /locus_tag="OO249_000469"
                     /pseudo
     CDS             21997..25470
                     /gene="bcsC"
                     /locus_tag="OO249_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            25552..27540
                     /gene="hmsP"
                     /locus_tag="OO249_000470"
     CDS             25552..27540
                     /gene="hmsP"
                     /locus_tag="OO249_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5634882.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            27723..29009
                     /gene="dctA"
                     /locus_tag="OO249_000471"
     CDS             27723..29009
                     /gene="dctA"
                     /locus_tag="OO249_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5634883.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPVSVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            29229..30725
                     /locus_tag="OO249_000472"
     CDS             29229..30725
                     /locus_tag="OO249_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5634884.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(30821..31750)
                     /gene="kdgK"
                     /locus_tag="OO249_000473"
     CDS             complement(30821..31750)
                     /gene="kdgK"
                     /locus_tag="OO249_000473"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5634885.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSTAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            31982..32749
                     /gene="pdeH"
                     /locus_tag="OO249_000474"
     CDS             31982..32749
                     /gene="pdeH"
                     /locus_tag="OO249_000474"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5634886.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            32819..34879
                     /locus_tag="OO249_000475"
     CDS             32819..34879
                     /locus_tag="OO249_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5634887.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEQLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(35113..36435)
                     /locus_tag="OO249_000476"
     CDS             complement(35113..36435)
                     /locus_tag="OO249_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5634888.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLLILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETQHQSL"
     gene            complement(36846..37859)
                     /gene="yhjD"
                     /locus_tag="OO249_000477"
     CDS             complement(36846..37859)
                     /gene="yhjD"
                     /locus_tag="OO249_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5634889.1"
                     /translation="MTQENEIKRPTQDLELEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMLGK
                     TQP"
     gene            complement(37908..38807)
                     /locus_tag="OO249_000478"
     CDS             complement(37908..38807)
                     /locus_tag="OO249_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5634890.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKNLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            39327..39929
                     /locus_tag="OO249_000479"
     CDS             39327..39929
                     /locus_tag="OO249_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5634891.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(39980..41629)
                     /locus_tag="OO249_000480"
     CDS             complement(39980..41629)
                     /locus_tag="OO249_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5634892.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVKRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            42033..43430
                     /gene="ccp"
                     /locus_tag="OO249_000481"
     CDS             42033..43430
                     /gene="ccp"
                     /locus_tag="OO249_000481"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5634893.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            43641..>43882
                     /locus_tag="OO249_000482"
     CDS             43641..>43882
                     /locus_tag="OO249_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5634894.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT        10540 a        11003 c        11926 g        10413 t
ORIGIN      
        1 atcccgttag cactaccgga aaattacccg ctgccgggga ttatagcggt attgctacca
       61 tacatgtcga actggagtaa cagtcatgaa gcgaacaata cttgcgttat gcctgctgcc
      121 tctttccttt tcgtcattcg cggcaaccac ggatttaggg ccgaaaggaa cattgaaatt
      181 cacccttaaa atcagccagg gtgcttgcga actggtgaaa gacagcgttg aggttgacat
      241 gggcaccgcg gttctcaaaa agccggtaag aatcggaacg gaaattaatc ccactccttt
      301 ttccatcggc ctgaaaaact gtagcgaagt ggtcagagcc tacgtgacta tggacggtac
      361 acccaatgca gacgactcaa atctttttgc gctggatgcg ggtggcgcta caggcgttgg
      421 gctgaaaatc aaaaccgctg ctggagtaca acagttcccg gaaaatacaa cgccgacacc
      481 ggttgagttt gcaattaacg gtgatggcgc acatcaactg aactatatcg ccagctacgt
      541 gcctattcgc gccgatgcaa ctattggtcg cgcagatgca acggtcgact ttactgtaca
      601 gtacgaataa tttcacatct taagggccag ttaactggcc ctttttactg ctttttgtaa
      661 cgctaaagtc tcttttcaaa cttgcatttt tgtaaatttg tgcttcatgc acactctttc
      721 cccacacttt ttcgctttgc tgtggtctac ttattcgcgc gtgtagattt tacttatctg
      781 actacctccg cacttttccc ctgccgggcc tgaaaagccg ctaagcaggg tgttatcacc
      841 tgtttgtcca gggtttgttt gcatgagata catcaaatcg attacacagc agaagctgag
      901 ctttttgctt gcaatctata ttggcctttt tatgaatggc gcggtttttt atcgccgctt
      961 cggcagctat gcgcacgatt ttaccgtctg gaaaggcgtt tctgctgttg ttgaactggc
     1021 cgccaccgta ctggtgacct tctttttact acgtcttcta tcgctgtttg gccgccgcag
     1081 ctggcgtatt ctggcgtcgc tggtggtgct cttttccgca ggtgccagct attacatgac
     1141 cttccttaat gtggtcattg gttatggcat catcgcttcc gtcatgacca ccgatatcga
     1201 cctgtcaaaa gaagttgttg gtctgaactt tattctctgg ttaatcgccg ttagtgcatt
     1261 gcctcttatc cttatctgga ataaccgctg tcgctacacc ttgctccgac aactgcgaac
     1321 cccagggcag cgtattcgca gcctggcggt cgtcgtactg gcgggtatta tggtttgggc
     1381 accgattcgt ttgctggata tccagcagaa gaaagtggag agggcgaccg gcgttgattt
     1441 gccgagttat ggcggtgtcg tagcgaactc ttatctgcca tcaaactggc tttctgcgtt
     1501 ggggctgtat gcctgggcgc gggtcgatga atcttccgat aataattcat tgcttaatcc
     1561 ggcgaagaag tttacatacc aggctccaca aaacgttgat gatacttatg tcgtgtttat
     1621 catcggtgaa accacgcgtt gggaccatat gggtattttc ggctatgagc gtaataccac
     1681 gccgaaactg gcccaggaga aaaatctggc ggcgttccgt ggttactcct gtgataccgc
     1741 aaccaaactc tcactgcgtt gcatgtttgt acgtcagggg ggcgcggaag ataatccgca
     1801 gcgcacatta aaagaacaga acattttcgc ggttctgaag cagttaggat tcagttctga
     1861 cctctacgct atgcagagcg aaatgtggtt ctacagcaac acgatggcgg acaacattgc
     1921 ttatcgtgag cagattggtg cggagccacg taatcgtggc aagccggtag atgatatgtt
     1981 gctggtagac gaaatgcagc aatcgctagg gcgcaacccg gatggtaagc atctgatcat
     2041 tctgcatacc aaaggttcgc attttaacta cacccagcgt tatccgcgta gcttcgcgca
     2101 gtggaagccg gaatgtattg gtgttgatag cggctgtacc aaagcgcaga tgatcaactc
     2161 ctatgacaac tcggtgacct atgtggatca ctttatctcc agcgtgattg atcaggttcg
     2221 cgataagaaa gcgattgtgt tctacgcagc tgaccacggt gagtcaatta atgaacgcga
     2281 gcacctgcac ggcacgccgc gtgaattggc accgccggag cagttccgcg taccgatgat
     2341 ggtctggatg tcagataaat atctggaaaa tccggccaat gcgcaggcat ttgcgcagct
     2401 gaaaaaagaa gccgacatga aagtgccacg ccgtcacgta gagctgtacg ataccatcat
     2461 gggttgtctt ggctatactt caccggatgg tggaattaac gaaaacaaca actggtgtca
     2521 catcccgcag gcaaaagagg cagcggctaa ctaagcggcc tgctgacttt ctcgccgatc
     2581 aaaaggcatt ttgctattaa gggattgacg agggcgtatc tgcgcagtaa gatgcgcccc
     2641 gcattcggtg attggcgcag cctggtagcg cacttcgttc gggacgaagg ggtcggaggt
     2701 tcgaatcctc tatcaccgac caaattcgaa aagcctgctc agcgagcagg cttttttgca
     2761 tttgaagccc atgaggatga gaacctccgg gggctgggag gttcgactcg acgtaggcct
     2821 gataagacgc gccagcgtcg catcaggcaa cggcaccgca cttattgtcg gatgcggcgc
     2881 gaccgcctta ttcgaccaac acaccacaca ttttcctgcc aactttgcgc cccatatctc
     2941 actatccatt aacggatttg tgacagaaac cattgtcttt ttttatattt gcttaaatct
     3001 ttttttgcgt tacttatggc tggcgctggc attttccgct gtgcgtttta gcgttcatgg
     3061 tattaatcgc tcagatatcc accttttcct cagatttttt tcattaaatg cataaaactt
     3121 aatcaccaaa tgttgctaaa tattaagctg attgttctgg ttatgttgga gtagcacaaa
     3181 tttctctgct gcaaatagct gtttgaagtt ttttaatctt tgtttgtggg ttctcaccgt
     3241 tggtgtaaat cccggttggc tgtattgacg ttttcacatt ctgttgacag attgtaggtc
     3301 acgaggggca ttttatggag gatccgcact gttacactga tgttaattag tacggcatcc
     3361 ccacctcata acgttgaccc gaccgggcaa aaaacaaaaa aggtcaggta gcgacaaccc
     3421 actgcaaagg gttaaaacaa caaacatcac aattggagca gaataatgcg tatttccttg
     3481 aaaaagtcag ggatgctgaa gcttggtctc agcctggtgg ctatgaccgt cgcagcaagt
     3541 gttcaggcta aaactctggt ttattgctca gaaggctctc cggaagggtt taacccgcag
     3601 ctgtttacct ccggcaccac ctatgacgcc tcttccgtac cgctttataa ccgcctggtt
     3661 gaatttaaaa tcggcaccac tgaagtgata ccgggcctcg ctgaaaagtg ggaagtcagc
     3721 taagacggta aaatctatac cttccatctg cgtaaaggtg tgaagtggca cgacaataaa
     3781 gaattcaaac cgacgcgtga actgaacgcc gatgacgtgg tgttctcgtt cgaccgtcag
     3841 aaaaacgcgc aaaacccgta ccataaagtc tctggcggca gctacgaata cttcgaaggc
     3901 atgggcttgc cggagctgat cagtgaagtg aaaaaggtgg acgacaacac agttcagttc
     3961 gtactgactc gcccggaagc gccgttcctc gctgatctgg caatggactt cgcctctatt
     4021 ctgtcaaaag aatatgctga tgcgatgatg aaagccggta caccggaaaa actggacctc
     4081 aacccaatcg gaaccggtcc gttccagtta cagcagtacc aaaaagattc ccgtatccgc
     4141 tacaaagcgt ttgatggcta ctggggcacc aaaccgcaga tcgatacgct ggtcttctct
     4201 attacccctg atgcttccgt gcgttacgcg aaattgcaga agaatgaatg ccaggtgatg
     4261 ccgtacccga acccggcaga tatcgctcgc atgaagcagg ataaatccat caacctgatg
     4321 gaaatgccgg ggctgaacgt cggctacctc tcgtataacg tgcagaaaaa accgctggat
     4381 gacgtgaaag ttcgccaggc actgacctac gcggtgaaca aagacgcgat catcaaagcg
     4441 gtttatcagg gcgcgggtgt atcagcgaaa aacctgatcc cgccaaccat gtggggctat
     4501 aacgacgacg ttcaggatta cacctacgat cctgaaaaag cgaaagcctt gctgaaagaa
     4561 gcgggtctgg aaaaaggttt ctccatcgac ctgtgggcga tgccggtaca acgtccgtat
     4621 aacccgaacg ctcgctgcat ggcggagatg attcaggcag actgggcgaa agtgggcgtg
     4681 caggccaaaa tcgtcaccta cgaatggggt gagtacctca agcgtgcgaa agatggcgaa
     4741 catcagacgg taatgatggg ctggactggc gataacgggg acccggataa cttcttcgcc
     4801 accctgttca gctgcgccgc ctctgaacaa ggttctaact actcaaaatg gtgctacaaa
     4861 ccgtttgaag atctgattca accggcgcgt gctaccgacg accataacaa acgcgttgaa
     4921 ctgtacaagc aatcgcaggt ggtgatgcac gatcaggctc cggcactgat catcgctcac
     4981 tccaccgtgt ttgaaccggt acgcaaagaa gtcaaaggct atgtggttga tccattaggc
     5041 aaacatcact tcgaaaatgt ctctatcgaa taattaaaag ccatacaaga ctgatggtaa
     5101 aggcaaaact gcctgatgtg ctccgcttat caggcctacg aaaattctgc aatgtattga
     5161 atttgcacga ttttgtaggc cggataaggc gttaacgccg catccggcat aaacaaagcg
     5221 cactttgtca acaatctgta tacccggtgg cgctgtgcct ctgcgtgcag cgccatccgg
     5281 cagcaatact tcattctctg tccgacaagg gcgggaattg atttgtgagc aatacagaca
     5341 cgcagttcca ggctgcgggt cactatagag aatccgggtt atgttgcagt ttattctccg
     5401 acgtttggga ctcgtcatcc ccacgtttat cggtattacc cttctcacat ttgcctttgt
     5461 ccacatgatc ccgggcgatc cggtgatgat catggcaggc gaacgtggga tctcccctga
     5521 gcgtcacgcg cagctgctgg ctgaactcgg cttagataaa ccgatgtggc agcagtatct
     5581 ccattacatt tggggcgtaa tgcacggcga tctaggcatt tcaatgaaaa gccgaattcc
     5641 agtatgggaa gagttcgtgc cgcgctttca ggccacgctg gaacttggcg tctgcgcgat
     5701 gatttttgct acggcagtcg gtattccggt tggtgtgctg gctgcggtta aacgcggttc
     5761 cattttcgat cacactgcag ttggcctggc gctgaccggc tactcgatgc cgatcttctg
     5821 gtggggcatg atgctgatca tgctggtttc ggtgcactgg aacctgacgc ccgcctccgg
     5881 tcgcgtgagc gatatggtgt tcctcgatga ctccaatccg ttaaccggtt ttatgctaat
     5941 cgacaccgcc atctggggtg aagacggcaa ctttatcgat gccgtcgccc atatgatctt
     6001 gcctgccatt gtgctgggta ctattccgct ggcggtcatt gtgcgtatga cacgctcctc
     6061 gatgctggaa gtgctgggcg aggattacat ccgcaccgcg cgcgccaaag ggctaacccg
     6121 catgcgggtg attatcgtcc atgcgctgcg taacgcgatg ctgccggtgg tgaccgttat
     6181 cggcctgcag gtgggaacat tgctggcggg ggcgattctg accgaaacca tcttctcgtg
     6241 gcccggtctg ggacgctggt tgattgacgc actgcaacgc cgcgactatc cggtagtgca
     6301 gggcggcgta ttgctggtgg cgacgatgat tatcctcgtc aacttgctgg tcgatctgct
     6361 gtacggcgtg gtgaacccgc gtattcgtca taagaagtaa ggggacatca tgtcacaggt
     6421 tactgaaaat aaagtgatta gcgcaccggt gccgatgacc ccgttacagg agttctggca
     6481 ctattttaaa cgcaacaaag gcgcggtcgt cgggctggtt tacgtcgtca tcgtgctgtt
     6541 catcgcgatc tttgccaact ggattgcacc ctataacccg gcggaacagt tccgcgatgc
     6601 actgctcgcc ccgccagcct ggcaggaagg cggcagcatg gcgcacttgc tgggcaccga
     6661 tgacgtaggc cgtgatatgc tgtcgcgcct gatgtacggt gcgcgcctgt cgctgctggt
     6721 tggctgtctg gtagttgtgt tatcgctgat tatgggcgtt attctcggcc tgatcgccgg
     6781 ttactttggc ggcctagtcg ataacatcat tatgcgcgtg gtcgatatca tgctggcgct
     6841 gccaagtctg ctgctggcgc tggtgctggt ggcaattttc ggcccgtcga ttggtaacgc
     6901 cgcgctggca ctgaccttcg ttgccttgcc gcactatgtg cgcttaaccc gcgccgccgt
     6961 gctggtggaa gttaaccgcg attacgtcac cgcgtctcgc gtggcgggtg ccggggcgat
     7021 gcgtcagatg tttattaaca tcttcccgaa ctgccttgcg ccgctgattg ttcaggcgtc
     7081 gctcggtttc tctaacgcca ttctcgatat ggctgctctt ggtttcctcg gcatgggggc
     7141 acagccgcca acgccggagt ggggcaccat gctctccgac gtgttgcagt tcgcgcaaag
     7201 cgcctggtgg gtcgtgacct tcccgggtct ggcgatcctg ctgacggtgc tggcatttaa
     7261 cctgatgggt gacggtctgc gtgacgcgct cgatcccaaa ctgaagcagt aagaggttcg
     7321 agatggcgtt attaaatgta gataaattat cggtgcattt cggcgacgaa agcgcaccgt
     7381 tccgcgccgt agaccgcatc agctacagcg taaaacaggg cgaagtggtt gggattgtgg
     7441 gtgagtccgg ctccggtaag tcggtcagtt ccctggcgat tatggggctg attgattatc
     7501 cgggccgcgt aatggcggaa aaactggtgt ttaacggtca ggatttgcag cgtatatcag
     7561 aaaaagagcg ccgcaacctg gtgggtgccg aagtggcgat gatcttccag gacccgatga
     7621 ccagccttaa cccgtgctac accgtgggtt tccagatcat ggaagcgatt aaggtgcatc
     7681 agggcggcaa caaaagtacc cgccgtcagc gggcgattga cctgctgaat caggtcggta
     7741 ttcccgatcc ggcttcgcgt ctggatgttt acccgcatca gctttccggc ggcatgagcc
     7801 agcgcgtgat gatcgccatg gcgattgcct gtcgaccaaa actgctgatt gccgatgagc
     7861 cgaccaccgc gctggacgtg accattcagg cgcaaatcat cgaactactg ctggagctac
     7921 agcagaaaga gaacatggcg ctggtgttga ttacccatga cctggcgctg gtggcggaag
     7981 ctgcgcataa aatcattgtg atgtatgcag gccaggtggt ggaaacgggc gatgcgcacg
     8041 ccatcttcca tgcgccgcgt cacccgtata ctcaggcatt gctgcgtgcg ctgccagaat
     8101 ttgctcagga caaagaacgt ctggcgtcgt tgccaggtgt cgttcccggc aagtacgacc
     8161 gcccgaacgg ctgcctgctt aacccgcgct gcccctatgc cactgacaga tgtcgcgctg
     8221 aagaaccggc gctgaatatg ctcgctgacg ggcgtcagtc caaatgccat tacccacttg
     8281 atgatgccgg gaggccgaca ctatgagtac gcaagaggcc accctgcaac aaccgctgtt
     8341 gcaggctatc gacctgaaaa aacattatcc ggtgaagaaa ggcatgttcg cgccggaacg
     8401 tctggttaaa gcgctggatg gcgtttcgtt taaccttgaa cgtggcaaaa cgctggcagt
     8461 agtgggcgaa tctggctgcg gtaaatcgac cctcggtcgg ttgctgacga tgattgaaac
     8521 gcccaccggt ggcgagctgt attaccaggg gcaggatctg cttaagcacg atccgcaggc
     8581 gcagaagctg cgtcggcaga aaatccagat cgtcttccag aacccttacg gttcgctgaa
     8641 tccgcgtaaa aaagtcgggc aaattcttga agagccgctg ctgatcaaca ccagcttaag
     8701 caaagaacag cgtcgggaaa aagccctgtc gatgatggcg aaagtcggcc tgaaaaccga
     8761 gcactatgac cgctatccgc atatgttctc cggcggtcag cgtcagcgta tcgccatcgc
     8821 ccgtggtctg atgctcgacc cggatgtggt gattgccgat gaaccggttt ccgcgctgga
     8881 tgtttcagtg cgcgcgcagg tgctgaatct gatgatggat ttgcagcagg agttggggct
     8941 gtcttatgtc tttatctccc acgacctgtc ggtggtggag cacattgctg atgaagtgat
     9001 ggtgatgtac ctgggccgct gcgtggagaa gggaacgaaa gaccaaatct tcaataaccc
     9061 gcgccatccg tacactcagg cgctactttc cgcgacgccg cgcctgaacc cggacgatcg
     9121 ccgcgagcgc atcaagctca gcggtgaact accaagccca ctgaatccac cgccgggttg
     9181 cgccttcaac gcccgctgtc gtcggcgctt cggcccctgc acccagttgc agccgcagct
     9241 aaaagactac ggcggtcaac tggtagcttg ttttgctgtt gatcaggatg aaaatccgca
     9301 gcgttaactc acaaaaccgg ggccatgcgc tccggtttac tcaatcagct tcaacagtgg
     9361 cgacaagcat agcaaaatgc cgtaaagcag aattagccag gctttaaatc cgcgaagttt
     9421 ttccagttgt gcgactttat agatcaggaa aaacggaatg aggcacgaca caatgccata
     9481 taacgggctt cccaactgaa agaacaccag caccgataca cgaaacgaaa cccaaatcgt
     9541 caacgtaatg acgataaaag cgcagatcgc cagagtcagc atgcgtgagt taattttctt
     9601 ggtatcaata attcggctta acagattgag aataatgcct ttaatagcct cgtggaaacc
     9661 gagataaatg ccaaagaatg cggtcagtac ggcaaagata ttcagcaccg tagaggtgat
     9721 atgaatgata tgtccaggga tcacctgcgc ggccagtgcc agtgccgaga tattttgttc
     9781 aaaggcagaa acggcttctt cgtggctaat tgagaaggta aacgaaaagg caaaaaacag
     9841 gatcaccgcg atgagcgtga tataactaat tcggtgggta cgcagcgcga gccgggttgc
     9901 cagtacttta tctgcttccc gtttacgata ggcaatattc attgggttaa gtacctgaat
     9961 aaataccgca gaaaagaagc aaaatggaat tgtaagcagg acatcgcgga aaaagacgga
    10021 ggcttgcggg aaggcggtta tattggcgaa attccagtgc gggatcatcg caaaaccgaa
    10081 cacgacaata atccctactt tgaccaccac cattggcccg gaaatcttaa acagtaatcg
    10141 ttcaccacca gacgcaatcg ctaccagtac ggcgaaaata gcgactttat aaagtagaga
    10201 ttgtgaaaga tcggcatcgg ttaaaccgaa ggttttcagg tacgaggcgc tgtcgaaaac
    10261 cacggagaga gagtagataa aaatcccgtg gataatcatc aaaaagtaga taaccccgag
    10321 gaaaattccc cagttcttcc ccaggtaatg actgataata tcggtgtagt cattacagga
    10381 atcgctttca gaaagggttt ttaaataaat gtcctgcact acccaggtgg caggataagc
    10441 aatgatcgct gcggtaataa atacccaaat tcccttcaag ccaatttgta ctggcatcag
    10501 cacggttccg gcaccaatcg ccatgcctat gcataataaa acccagccga agtcgtagcg
    10561 tgtaaacggc aatttctgat tgtgtttcga tagtgtgttg tgctgcataa agtcctgtcc
    10621 agaaagaagt cggcctcgca aaaatgaata aacccagttt ctcttgcgca acaggtggag
    10681 aagtcgaata cgcagtatgt aaaaaatgag gcaacagatt cactggggag gtcgcgagga
    10741 ggcgcaattc tacaaaattg cgcatttcac taccagtctg gagtcagccc ggatatgaac
    10801 tgcaactatt gcaaataaag aaattaagct ggaaggcatc gcgcaatgag gaagattgcc
    10861 gggtgctgac agtaaaaagc aatcgtaaaa tcagcggcaa gccgaaaggg ttccggtgag
    10921 gcgcaatgtt gcgggggctt tatccctggt ggcattggtt gctggagaga gaaaaccccc
    10981 gcacgttgca ggtatgcacc tgacaacatc acgggggcta atcttgaacc caaacaactc
    11041 aagaatagcc gcgaaacgtt ggcattacaa cacaggcggc tatatgacgc tcgcagaact
    11101 gggcatggcc ttctggcatg atttagcggc tccggtcatt gctggcattc ttgccagtat
    11161 gatcgtgaac tggctgaata agcggaagta acgtgtcatg cgggcgtcag gctgccgtaa
    11221 tggcaatttg cgcccggatc aggccgcaga ggttaaactc tgcggccttt ttcgtgctta
    11281 ctgcgggtaa ggcacccagt cgccgccgtt caggcgaacg tacggtttat cctggtattg
    11341 aataactact gcatttgagt tctcggagac cggtgctgtt tgtggcaacc cactggtgag
    11401 ttttttccag tcaacattgt cttcggtgaa aatcttgcca tcaagaacgc gaaccaccag
    11461 atcggagata gccaggaagc tgctcggttg gtcgatgaca atcggtgccc cctgatgtgg
    11521 tgccttcatg ccgaagaatt tcaccccaac ggggacgtct gtgatagacg ggctagggat
    11581 atcacgtagg ccagatacct gcattctgtc gcccttcagc gcgccgccgt gttccggcac
    11641 cacgaccacc atcactttac gacccgattt ctccagttca gtaaagaagg cgtccagttc
    11701 atcaaagaat ttctgcgccc gcgctttgta atccgctgtt ttgctgaccc ccggataatg
    11761 gttgccgtca tgcagtggaa gcgtgttgta gaacgtggca ctacggctgt ttttatcttt
    11821 ttcggtaacg tccagccagc ggttaagcac ggtggtatcg tcataaaccg gcgaaccatc
    11881 aaagcccagc aaaataaccg gcagatttgt ttgatccatc aattcagtct gcatgccgcc
    11941 attttcgcga acttctttca aaaaaccgcc gaactggccg ttatgtccca tcatcaggtg
    12001 ctgggtaaag cccagtttcg aaaggttatc aaacagatag cagtcgttat ttgccggttg
    12061 atacagatta gtgtgcgaag tctgcccgca gctggcgcgc agtaaacgga tcgccgccgg
    12121 gccactgtag gaggtggcgg agttaaagtt cttgaactca atatcgaaat gcgaccacag
    12181 tggatgcgac atcaacccgg cggcttctat atccgaccag gaaagcgaac agatgttaat
    12241 caccagtagt tcaaatggct gagcatcagc gggcagcgaa gacgggaagg tcgatttacg
    12301 tttcgcctcc gcgttataga aattattcag ccaggcgtta aggttcgccg ttgttggcgg
    12361 tgtagtttgt gcgggcatat cacccactac cggtgcgcca cccgtcgtct caacggttgc
    12421 cgctgcgtta ccacccgtcg ttgttacagt ggtcgtcggt tgtccggctg gccacaagga
    12481 gaaacttggt cccgccaggg taagtacgtt cagccatagc agtatggcaa ccacaaaaac
    12541 ggtaatgcga atccattgtg acaggaataa ccaggccact aataaaacaa aaatggcccc
    12601 aatcatctgc cagttaataa agcgtgtgac aaggtcgatt aaataatcgg tactgaaccc
    12661 cgccacctgc gaaccctggc tcattatgct ttccgggcca ggcaaccagg tgtcatgcca
    12721 gaacaaagca aagccgatcg gcagggcaat ccagtggcgc aagcgatgca ggctgtagcg
    12781 cggaatgggc atcagcagaa acgcggcaaa caccaaattg aggagcggat ggaagttaag
    12841 atatcccgcc cacaacaggc cgaacttaac cagaaaatag aagttccagc cggaaaggcc
    12901 gcgccagtat tgccagaggg aagaaggcat ggcggtattt tgcgtaaatt gagtcatttt
    12961 ttggttgccc tggctttttc cgtgcggcgt aatgtcccgg ccggtttaac ataacgaggt
    13021 ttagcaaaga acaaacgtaa ggtgtcgcga atgcgtcgca aagagtgccg cgccagatag
    13081 cccagcggga aaaatatcag tgcgcaaacg acaataattt caatgatatc gctgatggtc
    13141 atcatgatga gcgctccaca gcatcatcta acagtcgcat gggttctggt attcgtcgcc
    13201 agtggcgacc atcgtgctcg gcattgatga ccggtttaga actttgcgtt aaaggcagcg
    13261 gcatgcccca ttgttctggg gcaagcaggc gcatctgcac cagctcggca ctgatttgat
    13321 catcttcaaa ccagaccata cggtttgaga aaatgtcgcc agtaggcaat gggaaaatat
    13381 gattcaacgc ggtatccaga tcgttaatcc gacagaatga gagaaacagc accagccgat
    13441 taccgccaat ggtcatgata tcgccggtac ggttagggcg acacagcgtc agggcttgtt
    13501 caacgcggat acccggtacc ggacgtaggg caaccagaac gcctttaccg tgggcaggta
    13561 atagagggtt attcatcatg ttgttgacgg cattacagaa cacatcccac ttctggaaac
    13621 cacgcagttt gagcggctgg gtcattgaca gcaaggtagt gatatcttcc ggcacatagc
    13681 gactaaactt ctgcccttgc acgctttcga tcatcgtcag acaacgggag agtggcgcat
    13741 tccacggaat aaccatattt gcaccgcagg ccaataacaa acgttcatcg gtggcgcgca
    13801 ggctagcggt attttcccgc acgaggattt tcatcgcact accgcgctgg cgacgcaggg
    13861 tatgaatgct gcgggccagt ggctcgattt gcgcattttg ctgtaaagaa aagaccaccg
    13921 tcgccgcctg agcggtacgg gcttcattga acaggacttc gttattgttg aacagttgcc
    13981 agtgttccga tagcggcggc gcaccttcca gtacagcaac attactcaga atgcgttttt
    14041 cgtcgctgcg tggttggatc tccgcctctt cgctttgaac taatgtccag ataccatttt
    14101 gttgctgaac gctaagctgc tgacgggcgc tgaccccttt ttcgttgcac cagaaggcaa
    14161 tatccagcaa atgttggtca ccctgaaaac gcaaactggc aagaccaaaa agtgaacggt
    14221 attcctcaag caacaatgaa aattgtttat cgttattatt tccgggatta attaccaaaa
    14281 gcgaacaatg ggttaacctg ctccatttat tcattttatc caaccatgag cgaagccgct
    14341 cggcaggaat gttttgccat gcgttatttg cgcaaacaag aataaaaagg taattatggg
    14401 gatcaataga acactgcaaa tcacgggtca aatagtatag acctttttca tgatttagca
    14461 ttgaaaataa ttttattttt tccggccctt gataatcatc taattgaaag attttcgccg
    14521 gatcgctgtc catgctaatg accgcgacgt gtgcggtttc agcctgggat gcaattgttt
    14581 gattcgccag actgatagca tcttcatggc gatcgacgtt aaaccaccag acgccgcctg
    14641 ctggcatatg tcgcagctca tcccataatg atgagatacc gatagagaat acagggccca
    14701 caatgtccct catgtcacca ttttgtctga ttatcagttt actagcgaaa gctaagaaat
    14761 aaacctacca ttgaaattaa aacttatcag atttagcatg tgaatcaatt tagaaggtca
    14821 caagacgtgt gattttagtc ttttcgtttt taactttttc tgttgcgtaa cggaaagtca
    14881 aaaagtgagc aaattcccgt ctcgccgtaa acaataaacc ggcgaaatgc tcacggttag
    14941 aattgtctca tgaacggtac ggttatttca tagggatcaa gcaaaatgaa taacaatgaa
    15001 ccagatactc tgcctgatcc cgcgataggc tatatcttcc agaatgatat tgtggcgtta
    15061 aagcaggcat tttcactgcc tgatattgat tatgccgata tttcccaacg cgaacagttg
    15121 gccgcggcat taaaacgctg gccgttgctg gcagagtttg cgcaacaaaa gtaggggatt
    15181 ggtgaatggc cgtactggga ttgcaggggg tgcggggagg cgtggggaca acaaccatca
    15241 ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgtcctggtg gtcgatgcct
    15301 gcccggacaa cttgttgcgc ctgtcattta atgttgattt tacccaccgt cagggctggg
    15361 ccagagcgat gctggatggc caggactggc gtgacgctgg gttgcgctac acctcgcagc
    15421 tcgatttgct gccttttggt cagttatcca ttgaagaaca agaaaatcca cagcacaggc
    15481 aaacccggct gagcgatatt tgctccggct tacagcaact aaaagccagc gggcgttacc
    15541 agtggatttt aatcgactta ccgcgtgatg cctcgcagat aacccaccag ctgctgagtt
    15601 tgtgcgatca ctcgctggca atcgtcaatg tggatgccaa ctgccatatc cgactgcatc
    15661 agcaagcgct gccggatggc gcacatattt tgattaatga cttccgtatt ggcagtcagg
    15721 ttcaggacga tatttaccag ctttggttgc aaagccagcg ccgattactg ccgatgctca
    15781 ttcatcgtga tgaagcgatg gctgaatgcc tggcggctaa gcaaccagta ggtgaatatc
    15841 gcagtgatgc gctggcggct gaagagatac tgacgctggc gaactggtgc ctgttgaact
    15901 actccgggct gaaaacgcca gtcgggagtg catcatgagt atcctgaccc ggtggttgct
    15961 tatcccgccg gtcaacgcgc ggcttatcgg gggttatcgc gattatcgtc gtcacggtgc
    16021 gtcggctttc agcgcgacgc tcggctgttt ctggatgatc ctggcctgga tttttattcc
    16081 gctggagcac ccgcgctggc agcgtattcg cgcagaacat aaaaacctgt atccgcatat
    16141 caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat ctcattcaaa catgctggtt
    16201 actgatcggt acatcgcgca aagaaacgcc gaaaccgcgc aggcgggcat tttcaggtct
    16261 gcagaatatt cgtggacgtt accatcaatg gatgaacgag ctgcctgagc gcgttagcca
    16321 taaaacacag catcttgatg agaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg
    16381 gttgatcctc ggtatcatcg tcaccttctc gctgattctg gcgttaatct gcgttactca
    16441 accgtttaac ccgctggcgc agtttatctt cctgatgctg ctgtggggtg tagcgctgat
    16501 cgtacggcgg atgccggggc gcttctcggc gctaatgttg attgtgctgt cgctgaccgt
    16561 ttcttgccgt tatatctggt ggcgttacac ctctacgctg aactgggacg atccggtcag
    16621 cctggtgtgc gggcttattc tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct
    16681 cggctacttc caggtcgtat ggccgctgaa tcgtcagccg gtgccattgc cgaaagatat
    16741 gtcgctgtgg ccgtcggtgg atatctttgt cccgacttac aacgaagatc tcaacgtggt
    16801 gaaaaatacc atttacgcct cgctgggtat cgactggccg aaagacaaac tgaacatctg
    16861 gatcctcgat gacggcggca gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa
    16921 atatatcgcc cgcaccactc atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa
    16981 atatgccaaa ggcgagttcg tatcgatttt cgactgcgac cacgtaccaa cgcgatcgtt
    17041 cctgcaaatg accatgggct ggttcctgaa agagaaacag ctggcgatga tgcagacgcc
    17101 gcaccacttc ttctcgccgg acccgtttga acgcaacctg gggcgttttc gtaaaacgcc
    17161 gaacgaaggc acgctgttct atggtctggt gcaggatggc aacgatatgt gggacgccac
    17221 tttcttctgc ggttcctgtg cagtaattcg tcgcaaacca ttggatgaaa ttggcggtat
    17281 tgctgtcgaa acggtaaccg aagatgcgca cacgtctctg cgcctgcacc gtcgtggcta
    17341 cacctctgca tatatgcgta ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc
    17401 gcatatcggt cagcgtattc gctgggcacg cgggatggtg caaatcttcc gtctcgataa
    17461 cccgcttacc ggtaaagggc tgaaattcgc ccagcggctg tgctacgtca acgccatgtt
    17521 ccacttcttg tcgggcattc cacgactgat cttcctgact gcgccgctgg cgttcctgct
    17581 gcttcatgcc tacatcatct atgcgccagc gttgatgatc gccctgttcg tgctgccgca
    17641 tatgatccat gccagcctga ccaactcgaa gatccagggc aaatatcgcc actctttctg
    17701 gagtgaaatc tacgaaacgg tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct
    17761 gattaacccg cacaaaggca aatttaacgt caccgccaaa ggtggactgg tggaagaaga
    17821 gtacgtcgac tgggtgatct cgcggcccta catcttcctt gttctgctca acctggtggg
    17881 tgttgcggtc ggcatctggc gctacttcta tggcccgcca accgagatgc tcaccgtggt
    17941 cgtcagtatg gtgtgggtgt tctacaacct gattgttctt ggcggcgcag ttgcggtatc
    18001 ggtagaaagc aaacaggtac gccgatcgca ccgcgtggag atgacgatgc ccgcggcaat
    18061 tgcccgcgaa gatggtcacc tcttttcgtg taccgttcag gatttctccg acggtggttt
    18121 ggggatcaag atcaacggtc aggcgcagat tctggaaggg cagaaagtga atctgttgct
    18181 taaacgcggt cagcaggaat acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga
    18241 agttgggctg aaattaatgc cgctcaccac ccagcaacat atcgattttg tgcagtgtac
    18301 gtttgcccgt gcggatacat gggcgctctg gcaggacagc tacccggaag ataagccgct
    18361 ggaaagtctg ctggatattc tgaagctcgg cttccgtggc taccgccatc tggcggagtt
    18421 tgcgccttct tcggtgaagg gcatattccg tgtgctgact tctctggttt cctgggttgt
    18481 atcgtttatt ctgcgccgcc cggagcggag cgaaacggca caaccatcgg atcaggcttt
    18541 ggctcaacaa tgatgataac gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta
    18601 tggggatgag tgcgttcccc tctttcatga cgcaggcgac gccagcaacg caaccactga
    18661 tcaatgctga gccagctgta gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga
    18721 tgccgggcgt gcagggcgct gatgcgccag tcgtggcgca gaacggtcct tcgcgtgatg
    18781 tgaagctgac ctttgcgcaa attgcgcctc cgccgggcag catggtgcta cgtggcatta
    18841 acccgaacgg cagcattgag tttggtatgc gcagcgatga agtggtgacg aaggcgatgc
    18901 tcaacctcga atacatgcca tcgccatcgt tactgcctgt ccagtcgcag ttaaggttta
    18961 tctcaatgat gagctgatgg gcgtactgcc agtgaccaaa gaacagttgg gtaaaaaaac
    19021 gctggcgcaa atgcccatta acccactgtt tattaccgac ttcaaccgtg tgcggctgga
    19081 gtttgtcggc cattatcagg acgtgtgcga aaacccggcc agaaccacgc tttggctgga
    19141 tgttgggcgg agcagtggac tggatctgac ctatcagacc ctgaatgtga agaatgacct
    19201 gtcacacttc ccggtgccat tctttgaccc gcgcgataac cgtactaaca ccttgccgat
    19261 ggtctttgcg ggtgcgccgg atgttgggct gcaacaagca tctgccattg tcgcctcgtg
    19321 gtttggttcg cgttctggct ggcgtgggca gaacttcccg gtgctctata accaactgcc
    19381 ggatcgtaat gccattgtct ttgcaaccaa cgacaaacgg ccggacttcc tgcgcgatca
    19441 tccggcggta aaagccccgg tgattgagat gatcaaccat ccgcagaatc cttacgtcaa
    19501 actgctggtg gtgtttggtc gtgacgacaa agacctgttg caggcagcga aaggtatcgc
    19561 tcagggtaac attctgttcc gtggtgaaag cgtggtagtg aatgaagtga aaccgctgct
    19621 accgcgtaag ccgtacgatg cgccgaactg ggtacgtacc gatcgtccgg tcacatttgg
    19681 cgaactgaaa acctatgaag aacagttaca atccagcggt cttgagccag cagcgattaa
    19741 cgtttcgcta aacctgccgc cggatctcta cctgatgcgc agtaccggca ttgatatgga
    19801 tattaattac cgctacacca tgccgccggt gaaagacagt tcgcggatgg atatcagcct
    19861 gaataaccag ttcctgcaat ccttcaacct gagcagcaaa caggaggcga accgcctgct
    19921 gctgcggatt ccggtattac aaggtttgct ggatggcaaa acagatgtct ctattccggc
    19981 gctgaaactg ggcgcgacca accagctgcg cttcgacttt gagtatatga acccgatgcc
    20041 gggcggttcg gtggataact gtattacctt ccagccggtg cagaatcatg tggtgattgg
    20101 tgacgactcc accatcgact tctcgaagta ttaccacttc atcccgatgc cggatctacg
    20161 cgcctttgct aacgcgggct tcccattcag ccggatggcg gatctgtcgc aaaccatcac
    20221 cgtgatgccg aaagcgccta acgaagcaca gatggaaacg ttgctgaata ctgttggttt
    20281 tatcggcgca cagacgggct tcccggcgat taatctgacg gtgaccgatg atggcagcac
    20341 cattcagggc aaagatgccg acatcatgat catcggtggt atcccggaca aactgaaaga
    20401 cgataagcag atcgacctat tggtgcaggc gaccgaaagc tgggtgaaaa caccgatgcg
    20461 ccagaccccg ttccccggca ttgtgccgga cgagagcgat cgcgcggcag aaacccggtc
    20521 aacgctgacc tcttccggtg cgatggcggc ggtgattggc ttccagtcgc cgtataacga
    20581 ccagcgcagc gtgattgcgc tgttggcaga tagcccacgc ggttatgaaa tgcttaacga
    20641 tgcggtgaac gatagcggca aacgcgccac catgttcggt tcggtcgcgg tgatccgcga
    20701 gtccggtatc aacagcctac gtgttggcga cgtttattac gtaggtcatc tgccgtggtt
    20761 cgagcgcgtg tggtatgcgc tggcaaacca tccgattctg ctggcggtgc tggcggctat
    20821 cagtgtgata ttgctggcat gggtactgtg gcgtctgctg cgaattatta gtcgtcgtcg
    20881 tcttaacccg gataacgagt aattgaagat gaatgtgttg cgtagtggaa tcgtgacgat
    20941 gctgttgttg gctgccttta gtgttcaggc agcctgtacc tggcctgcct gggagcagtt
    21001 taaaaaggat tacatcagtc aggaagggcg cgtcatcgac cccagcgacg cgcgcaaaat
    21061 caccacctcc gaagggcaaa gttacggtat gttctttgcc ctggcggcta acgaccgtgc
    21121 agctttcgat aatattctcg actggacgca gaacaatctc gctcagggtt ctttaaaaga
    21181 acgtttgccc gcctggctgt ggggcaagaa agagaacagt aagtgggaag tgctggacag
    21241 caattcggcc tccgatggtg atgtctggat ggcctggtcg ttgctggagg cggggcgttt
    21301 gtggaaagag cagcgttata ccgacatcgg cagcgcgttg ctaaaacgta tcgcgcggga
    21361 ggaagtggtg acggtgcctg ggctgggttc catgttgtta ccgggcaaag tgggttttgc
    21421 tgaggataac agctggcgtt ttaaccccag ctacctgccg ccgacgctgg cgcagtattt
    21481 cacccgcttt ggcgcgccgt ggactacgct gcgcgaaacc aatcaacgtt tattgctgga
    21541 aaccgccccg aaaggctttt cgccagactg ggtgcgttat gagaaagaca aaggctggca
    21601 gctaaaagcc gaaaaaacat tgatcagcag ctacgacgcc atccgcgttt acatgtgggt
    21661 aggcatgatg cctgacagcg atccgcagaa agcgcggatg ctcaaccggt ttaaaccgat
    21721 ggcgacattc actgagaaaa acggttatcc gccggaaaaa gtggatgtgg ctacggggaa
    21781 agcgcagggt aaaggaccag tcggtttttc tgccgccatg ctgccctttt tacaaaaccg
    21841 cgatgcgcag gccgttcagc gtcagcgcgt ggccgataac tttcccggca gcgatgccta
    21901 ttacaactat gtgctgaccc tgtttggaca aggctgggat caacaccgtt tccgcttctc
    21961 gacaaaaggt gagttattac ctgactgggg ccaggaatgc gcaaattcac actaaacata
    22021 ttcacgcttt ccctcggtct ggccgtcatg ccgatggtcg aggcagcacc aaccgctcag
    22081 caacagttgc tggagcaagt tcggttaggc gaagcgaccc atcgtgaaga tctggtgcaa
    22141 cagtcgttat atcggctgga acttattgat ccgaataacc cggacgtcgt tgccgcccgt
    22201 ttccgttctt tgttacgtta gggcgatatt gatggcgcgc aaaaacagct cgatcggctg
    22261 tcgcagttag cgccgagttc aaatgcgtat aaatcgtcgc ggactacgat gctactttcc
    22321 acgccggatg gtcgtcaggc actgcaacag gcacgattgc aggcgacgac cggtcatgca
    22381 gaagaagctg tggcgagtta caacaaactg ttcaacggtg cgccgccgga aggtgacatt
    22441 gctgtcgagt actggagtac ggtggcgaaa attccggctc gccgtggcga agcgattaat
    22501 cagttaaaac gcatcaatgc ggatgcaccg ggcaatacgg gcctgcaaaa caatctggcg
    22561 ctattgctgt ttagtagcga tcgccgtgac gaaggttttg ccgtcctgga acagatggca
    22621 aaatcgaacg ccgggcgcga aggggcctct aaaatctggt acgggcagat taaagacatg
    22681 cccgtcagtg atgccagtgt gtcggcgctg aaaaaatatc tctcgatctt tagtgatggc
    22741 gatagcgtgg cggctgcgca atcgcaactg gcagaacagc aaaaacagct ggccgatcct
    22801 gctttccgcg ctcgtgcgca aggtttagcg gcggtggact ctggtatggc gggtaaagcc
    22861 attcccgaac tacaacaggc ggtgcgggcg aacccgaaag acagtgaagc tctgggggcg
    22921 ctgggccagg cgtattctca gaaaggcgat cgcgccaatg cagtggcgaa tctggaaaaa
    22981 gccctcgcac tggacccgca cagcagcaac aacgacaaat ggaacagtct gctgaaagta
    23041 aaccgctact ggctggcgat ccagcagggc gatgctgcgc tgaaagccaa taatcctgac
    23101 cgggcagaac gcctgttcca gcaggcgcgt aatgtcgata acaccgacag ttatgcagtg
    23161 ctggggctgg gcgatgtggc gatggcgcga aaagattatc ccgccgccga acgttattat
    23221 cagcagacct tgcgtatgga cagcggcaac actaacgccg tgcgcgggct ggcaaatatt
    23281 taccgccagc aatcgccaga aaaagctgaa gcgtttatcg cctcgctctc tgccagtcag
    23341 cggcgtagca ttgatgatat cgaacgcagc ctgcaaaacg accgtctggc acagcaggca
    23401 gaggcactgg aaaaccaggg caaatgggcg ccggcggcag cacttcagcg gcaacgactg
    23461 gcgctggacc ccggcagcgt atggattact taccgacttt cgcaggatct ctggcaggcc
    23521 ggacaacgca gccaggccga tacgttaatg cgcaatctgg cgcagcagaa gtcgaacgac
    23581 ccggagcagg tttacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg
    23641 ctggcgcata tcaacagcct gccgcgagcg cagtggaaca gcaatattca ggagctggtt
    23701 aatcgactgc aaagcgatca ggtgctggaa accgctaatc gcctgcgaga aagcggcaaa
    23761 gaagctgaag cggaagcgat gctgcgtcag caaccacctt ccacgcgtat tgacctcacg
    23821 ctggctgact gggcgcagca gcgacgtgat tacaccgccg cccgcgctgc atatcagaat
    23881 gtcctgacgc tggagccaac taacgccgat gccattctgg ggctgacgga agtggatatt
    23941 gctgccggtg acacagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac
    24001 gcctcgctga acacacagcg gcgcgtggcg ctggcgcagg cgcagcttgg agataccgca
    24061 gcggcgcagc agacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg
    24121 gaaagcgcga tggtgctgcg cgacggtgcg aagtttgaag cgcaggcggg cgatccaaag
    24181 caggcgctgg aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg
    24241 caggataacg acacattcac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa
    24301 cgcggcgtgc gcagcgatgc ggcgggcctc tatcgccagc aggatcttaa cgtcaccctc
    24361 gagcacgatt actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact
    24421 accatgttgc aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc
    24481 gtcaatatga acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg
    24541 ggcacctgta cattacagga ctgtagtggc aaccgcagcc agtcggactc cggtgccagc
    24601 gtggaggtcg gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc
    24661 ttcaacgtgg tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt
    24721 tacaccgtta acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa
    24781 aaagactccc cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg
    24841 ctaagtctga gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac
    24901 cagttaaccg gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac
    24961 tataaggtca ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg
    25021 cattacgaca aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag
    25081 gaatacctgt cgtttgctat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg
    25141 gagctggggg cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg
    25201 ctgatgaatc tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc
    25261 ggcagcagtc agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc
    25321 aactggtttg ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat
    25381 ttcctgctct acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg
    25441 cagccgctga taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc
    25501 tgcgatcggg tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta
    25561 agtcgctcgt taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt
    25621 tttatttttt gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct
    25681 acgcaactgg aaagcattgc tcgctctgta cgcgaaccct tatcttcagc tattttgaaa
    25741 ggcgatattc ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc
    25801 cgtgccgatg tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag
    25861 cgcccggtgc cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc
    25921 gtttactcgc tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg
    25981 gattccttcc gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac
    26041 ttacttttgt cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg
    26101 catccgttac gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt
    26161 caccaactgg cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt
    26221 tacaacctca accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg
    26281 cgcttcccgg tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt
    26341 gtcgcgcgta aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact
    26401 gcgggcgtgc tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc
    26461 aaatcggtac tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc
    26521 attgccaacg gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcact
    26581 atcatgagcg agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc
    26641 gtggcgatgt tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg
    26701 gcatttaccg ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg
    26761 gaagccgccc agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat
    26821 cagtttgcta tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg
    26881 gaagtgttac tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc
    26941 gatcgcattg agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc
    27001 tgtcgattgc ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc
    27061 tctgcgctgc aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc
    27121 tatcgcattc aaccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac
    27181 cctcatgctg cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg
    27241 gatgatttcg gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca
    27301 atcgacgtac tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg
    27361 attgctgcaa ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg
    27421 gagactgaag cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc
    27481 ctttttgctc gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag
    27541 ctaccccaaa ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact
    27601 ttttaacctt tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt
    27661 attttaaggc cgcaggtacc tcataacctt acaagacctg tagttttact aaaggacacc
    27721 ctatgaaaac ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg
    27781 gtattctcct tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg
    27841 gcttcgttaa gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg
    27901 gcattgcggg catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt
    27961 actttgaaat tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc
    28021 agcctggtgc cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt
    28081 acgccgatca ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggtga
    28141 gcgtcattgg cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt
    28201 ttggttttgc gctccaccgt ctgggcagca aaggccaact gatttttaac gtgatcgaaa
    28261 gtttctcgca ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg
    28321 cgttcggggc aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg
    28381 ggcagctgat tatctgtttc tatattacct gtatcctgtt tgtggtgctg gtattgggtt
    28441 caatcgctaa agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac
    28501 tgctgattgt actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga
    28561 tggagaaact cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt
    28621 ttaaccttga tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca
    28681 ctaacagtca gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt
    28741 ctaaaggggc ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg
    28801 cggtgggcca tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt
    28861 cagaagctcg tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta
    28921 agtgggtgaa agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg
    28981 atggcaaaac gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc
    29041 gagtgcgggt gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc
    29101 aggtttattt cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt
    29161 ttaacgtcat ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag
    29221 gggttcacat gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca
    29281 ctgctggcta tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc
    29341 tttccaacgg tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg
    29401 aaattcgcct gctggttaat accggttcgc tcgccgaaag tacacaacag agcggttaca
    29461 gtcacgccat ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc
    29521 gttcattgtg gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt
    29581 atgacaccac gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag
    29641 cgctctctta tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc
    29701 acgcgctgca aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt
    29761 ggcgctatcg tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac
    29821 aacccgttga agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa
    29881 tgacgctact ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa
    29941 cgtttggcga actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc
    30001 tgcgtgcgga agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca
    30061 tgtgggatac gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg
    30121 cggacctggc ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca
    30181 gcaaagacat cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca
    30241 tcaacatcga atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac
    30301 tggcgaaggt tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa
    30361 agaaactgga gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga
    30421 tgggtcagcg gatgcgttcg ttgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc
    30481 agaaactgcg gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc
    30541 gtcagcagtt gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat
    30601 ttaacatgaa agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg
    30661 ccgccacctc tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac
    30721 agtaatgcct gatggtgatg agttagcaca accatcaggt atttatggat gagctggata
    30781 gaggggtaac actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac
    30841 gcgggataat cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg
    30901 ccgcgttttc cgcactgccg cccgtcaaac gtaccgccag ataaccggcg ctgaaagagt
    30961 cgccagctgc ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca
    31021 ctaacccttc gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt
    31081 ctttcacgcc cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca
    31141 gcgcgtcttc atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt
    31201 acacctgctg tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa
    31261 tcacttttcc gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg
    31321 tcgggcttaa gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct
    31381 cttcgcaaat cgccgtagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc
    31441 gccagtagta gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca
    31501 gacggttttc catccgttgg gtcagggaaa tatcaacgtt ctcgccgtgc caggcgtcca
    31561 gcatctgctg gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg
    31621 caggatcgac ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc
    31681 gcttaacgtc cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct
    31741 ttttggacat agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg
    31801 tttgctggtc aacaatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc
    31861 tgaaagattc cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct
    31921 tacgcgccga taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt
    31981 gatgataagg caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca
    32041 ggaacggcgt ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac
    32101 atgcgggcgg ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc
    32161 gcaacgcctg ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt
    32221 tgtgaaagag cagattgatt tgctggcgca aaaagccgac ttctttatag agcacggcct
    32281 gctggcatcg gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat
    32341 cctgcgccaa attgagcgtc ttccctggct gcgtttcgaa ctggtggagc atatccgtct
    32401 gccgaaagat tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tgggtgattt
    32461 tggtaccggg atggcaaatt tctctgcact aagtgaagtg cgttatgact acatcaaaat
    32521 cgcgcgagaa ctgtttgtga tgctgcgtca gtcgccggaa ggacgcacac tcttttctca
    32581 gcttttacat ctaatgaatc gctattgtcg cggggtgatt gttgagggcg tagaaacgcc
    32641 ggaagagtgg cgtgatgtac agaactcgcc cgcattcgcc gcacaaggct ggtttctttc
    32701 acgcccggca ccgatagaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt
    32761 ttccgcctgg ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat
    32821 gagcaaggca ggcaaaataa ctgctgcgat ttcaggggct ttcttgttgt tgattgtcgt
    32881 ggcgatcatt ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa
    32941 agtctctgcg gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga
    33001 gcggcaaaaa caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga
    33061 agacatcatt cttggcaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg
    33121 agtagaggca acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat
    33181 caagctcgaa aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac
    33241 gtttaatctc gccaacgatg ataacaaaga cgcgaatgca aagccgtcgg catggtcgtt
    33301 tcggctggat aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa
    33361 agcggatctg gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac
    33421 tggatcgaaa ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc
    33481 gcagggacgt tataacggtg aacagctcac gggtacggga aaaataggcg gtatgctggc
    33541 gctgcgtggc gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg
    33601 tgttgctttt gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct
    33661 taaattttct ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga
    33721 taccccgccg tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc
    33781 ggtctttgat tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct
    33841 ggtctacacc accggaaagc cgcgaccaaa actggaaggt gatgtcgagt cgcggcaatt
    33901 gcggctggcg gacctgggac cgttgattgg cgttgattcg gggaaagggg cagaaaagtc
    33961 gaaacggtct gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc
    34021 ttatgaccgc ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg
    34081 ccgacgtatc gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct
    34141 caaaaatgct gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc
    34201 ggcgaatatc catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc
    34261 tcgtcgactg aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg
    34321 ggaaatgaac ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg
    34381 caacagtaac ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat
    34441 ggagattgtt gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga
    34501 ggtgcgggtg aactgcgcgg cggcgaatct gaatattgcc aacggcgtag cgcgcccgca
    34561 gatttttgct ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc
    34621 ttcggaacag ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact
    34681 gcgttcgccg ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg
    34741 accgctgatt gcccgtggtg ccgttgcggc ggcactggca acgctggtaa caccggcggc
    34801 ggcgttattg gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt
    34861 gtcgcagatg aagaagtgat gaggttggat tgtgtttgct ggtgagttcg ggagcagcgt
    34921 gttcccctca ccctaatcct ctccccaaag gggcgaggag actgttttgt gcgtgttgaa
    34981 acaacgagta ccgcttttcc gttcactttc tgggggaggg ctgttatgtt cgcgatataa
    35041 catctcgctc cgcttttctc cctctccctt tcagggagag ggctggggtg agggtggctg
    35101 cccacacgat acttacaacg actgatgttg cgtctcatgg gtcagcagca gggcgattaa
    35161 cgtcaagcca gccatcgccg ccagatataa ccccaccgca cctaacccgt agttagtctg
    35221 caaccaggct gcgatatacg gcgcaacgga agccccgaga atcgacgata cgttgtaaga
    35281 gaacgatgct ccggtgtaac gcacttctgt cgggaacagc tctggtaaca gcgcgcccat
    35341 cggaccgaag gtcagcccca tcagacttaa ccccagcagc aggaaggcaa aaaccagaat
    35401 cgggttgcca gaaccgagca aagggttaaa ggcgaatagc gcgaacagga tgagcagcgt
    35461 ggtgatgatt accatgcttt tacggcgacc aaaggcatca gccagtaatc cagcgactgg
    35521 caccatcacg ccaaaaccaa taactgccat catcaacatc cacaacactt cgttacgcgg
    35581 caggccaagc ccaactggcg cggcggcggt actaaaggtc atggagtaga ccgtcatgat
    35641 gtaaaacagc gtataggttg ccagcataat gaacgtaccc agtacggtta cgcgaacatg
    35701 tttggtcagc agcgtaccca gcgggatctt cacctgtttt ttcgctttag cgactttctc
    35761 aaacaccggc gactcatgca gcgacacgcg aacatacagg ccgataatga ccagcaccgc
    35821 cgagaagata aatggcacac gccagcccca gctcataaac tgctcgtcgg tcagtagcca
    35881 ggaaagcagc aagaaagtgc cgttggcaaa gaagaagcca atcggtgcgc ccagctgcgg
    35941 gaaggagccg tacaatgcac gtttgcgcgg tggggcgttt tcagtcgcca gcagcgccgc
    36001 gccgccccat tcaccaccta agcccagacc ctgaccaaat cgagccagcg ccagcagcag
    36061 cggggcgaaa ataccaatcg tggcatagcc tggcagcaga ccaatcacca cggtcgaaat
    36121 ccccatcgtt agcaacgagg cgaccagcgt cgctttacgc ccaacgcgat cgccaaaatg
    36181 accaaaaacg gcagagccaa tggggcgcgc gacgaaggcg atggcgaagg tggcgagcga
    36241 ctgtagcgtt gctgccgtag gatcgccctg cgggaagaag atatgcggaa acacaataac
    36301 ggccgcagtg gcgtaaatgt aaaagtcgaa gaactcaatg gctgtgccaa tgagagaggc
    36361 gacaaggact ttattacgcg agttgatcgg cgtgtattct tgctcgtggt cgagtgttgt
    36421 ggctgttgct tgcataatct tttcttattt tttggctaac gaatagccat attacgcaca
    36481 gcaaaagcga tatttcaatg tgtaacaaat cgcggaaatg gtcaaaaacc aggcaaaaag
    36541 gggataatgt ttaggccagt gatttttctt ctatgaaatg cttcacataa ttttataaat
    36601 agtggataaa actggtttca ggttaaataa aagttaccaa ctggatttat atgctggggt
    36661 tatgagttag aatgaaattg tttgctccca aatagatggc tgaagaggct ctggggacga
    36721 gtttcaagag gttatgtgcg cattatagcc ggatgcgatg ctggcgcatc ttatccggcc
    36781 tacggggagc caggccgaat aaaatcgtat gccggataag gcgtttacgc cgcatccggc
    36841 ctattttaag gctgcgtttt ccctagcatt cgcgggtcgt ctttatattc ggcggtggca
    36901 atccacgccg cgcaaaacag cgtcaaacgg gcgaagaagt agaaaaatgc catcagcccc
    36961 agcacggaac caaatgctgc gccagagggg gatttcatca gcgatggcag ggtgtaggtc
    37021 atcacgattt taatcacttc aaaaccaata gcagccagaa atgttccgcg aatcagggct
    37081 tttttgcgcg gacggtggcg cggcaaccgc cagaagatcc agaagaaaag cagatagttg
    37141 gcgaagatgg aaatcgccag gccaatcaat cgccacgtcg gtttcagcca ctcaatgctg
    37201 ttcaggtgca gggcgctaat aatcatttgc tgcgccgaac cggcaaccga ggtgatcgaa
    37261 agcgtcacaa tcagcgcaat caacaaacca atcagtgaaa taaaatcacg cagatatttt
    37321 acccagaact tctcctgatc ttgcggcgag cgttcccaga catcacgcga ctgggcacga
    37381 atcgcttcac gcaggttacc catccagttg atgccggaat aaagtgccac cgccaggccg
    37441 acaagcccta cagtcgtacg ctgctgaacg gcggtgttga tggtgttttt caacgtggcg
    37501 gctagtgtcg gatcgctgat gttttgcaga attttgtcga agatatcctg tagcaacatc
    37561 ggatgggagg ccagcacaaa gccccctgcg gcaaacgaca ccatcaaaat cgggatcatc
    37621 gacaaaaatg agaaataggt gatagccgca ccaaactggt tacccagccg atcgttaaag
    37681 cgttctgtcg cgcgaatcag gtgcgcaata accggttggc gttggacttt ttcggcagtg
    37741 gtggtgacgg tttccagcgc ctggctaact ttgccgcctt tctcgctatt atccaactgc
    37801 ttaatcggct cgagctccag atcctgggtg ggacgtttga tctcgttttc ctgcgtcatc
    37861 cgctcttttg tccttctgtc gtggtcattc ttaaaaagta tagtcggtca gtccaggtgc
    37921 tctttcatta cgccagccag ccactgcata aacaggttta cacgccggga aagctcccga
    37981 cgctgcgggt aaaccagcga aagggagagc ggctcggcac ggtagccagg taatacttca
    38041 ataagccgcc cggcacgcag ggcttcgcgc acggcaatgc gcgggatctg aataatcccc
    38101 agaccggcaa gaccggcggc gagatagttt tcgctactat ttaccgtcaa catgccgcca
    38161 gacttaaacc actggacgcc attaacgctg gcaacctcaa aacctaacgg atgtacaccc
    38221 aggtgcggtg tataacgcac tattgcgtgt gaagtcagat catcggggct ttgcggataa
    38281 ccaaagcgcg tcagatagtg cggactggca cagttgacca tggtcagttt gccgagggga
    38341 cgggcgataa cgccatcttc cggtaacgcg ccagtgcgta tcacgcaatc aaaaccatca
    38401 tgaagaatat ctaccggacg gtcatggcta ctcagttcca gctcaattcc cggatgcaga
    38461 tagagaaatt ccgacaggcg cggcagtaac aggtttttcg cgattcctgg cgggatgtcg
    38521 atgcgtaatt taccgctgat actggtggca tcctgttgaa acagaccgtc cagttcgttg
    38581 agattactca acacatcttt tgcccgttga taataggtca tgccttctgg cgtgagtttg
    38641 acccgtcgcg tggtgcgctg gagaagctgg gtgccaagtt gatgttccag tgcctgtatc
    38701 tggcgcgaaa cacttccctt tggcaaagca aagaaatccg ctgcgcggga aaaactttcc
    38761 agctccgcga ctttgatgaa caactgcatt gcgtgaattt tatccatttc tttgaccgat
    38821 tgttgttcac agcgaaacag taacgcgcat atcgcattgt ttattgtttt ttctaacatc
    38881 taatgccggg tttcacaatg ttgtcatgac gaatgtggcg atttctcagc aggaaaaggg
    38941 cggaatagct ttatgagaaa ggtgaatccc cttaaacaga gcgtattttt cagtgattcc
    39001 ctttaaagtg aatagaggac gtattggcta tttgtgctta tttttgtctc attgccattt
    39061 gttattttgg cttgtagtcg cggaatttca tttttaattg catgcgtact aaccaccaat
    39121 ataaggaaat cctcaaaatt gttttaaata gtgccctcga catgtaattt caggcgttta
    39181 gctgtaacta tttatatcgg taactatttt ctacgcgtca tctctgtaga atggccaccg
    39241 ccaaatggcg aattcttatt ttttctcgcg ggtaataccc ttccactact taagtctttt
    39301 taacatattc aggtggagat tcagttatgc aaatagtcat gtttgacagg cagtcaatat
    39361 ttattcatgg aatgaaaatc agtttacagc agcgtattcc aggggtgagt attcaggggg
    39421 ccagtcaggc agacgagtta tggcaaaagc tggaaagtta ccctgaagcc ttagttatgc
    39481 tcgatggcga tcaggatggt gagttttgct actggttgct gcaaaaaacc gtggtgcagt
    39541 ttcctgaggt taaggtgtta atcacggcga cggattgcaa taaacggtgg ttacaggaag
    39601 ttatccattt taatgtgctg gccattgtgc ctcgtgattc aaccgtcgag acatttgcgt
    39661 tggcggtaaa tagtgcggcg atggggatga tgtttctgcc tggagactgg cggacgacgc
    39721 cggaaaagga cattaaagat ctcaaatcgt taagcgcccg ccagcgggag attttaacca
    39781 tgttagcggc gggcgagtca aataaggaga tcggcagagc gctgaatatc agtaccggaa
    39841 cggtaaaagc gcatctggaa tccctgtatc gccgtctgga agtgaaaaat cgcacccagg
    39901 cggcaatgat gttaaatatc tcctcctgaa gaggataatt ttacagccag caggaagtta
    39961 gcggctggct gtaaaaatat tatggttcgc cgtacaaacc aattaaacgg cgtaccacac
    40021 cgttagtcca gccaaaccca tcctgcaacg gatactcgcc accgccgcct tcgcgtggaa
    40081 caccatcggc aatatggtat ttttcgatca gtttgtgctg ttccagatag aactgattca
    40141 ccgtcttcag ccagcttcgc gcgatttcat cacccagaag gtcatcgccg tacattttaa
    40201 atccctgaat cgccatccat tgtaacggtg cccagccgtt gggtttatcc cactgttcac
    40261 cggtttcgta ctcgcttgcc agaatcccgc caggtgtcag taaccgactg cgcacggcat
    40321 ttgccagacg atcggcctgt tcatggttcg ccataccgac atagagtggc acaatggcgg
    40381 cagcggaaaa cagcgccagt tgttcgcgtc gccagtcgta atcgcggtag atgccgtttt
    40441 catcatccca gaggtaacgc tttaccgcat cgcgacgggc actggccttc tggcggaaca
    40501 gtgcttctgt ctctttctcg cctttcagcg ccgagatgtt ggcgatggcg ctctccagtt
    40561 taaacaggaa ggcattcaga tcgatgggga tgaactgggt ggtacgaata ctcgccagac
    40621 gaccagtatc acgcagccaa cgggaagagt aatcccaacc ggaggccgcc cccgctcgta
    40681 aatcgcggta cacctcgttg ggcgggcgac cagaatgttt cgcggtttca acgtcctcaa
    40741 gccaggattc gtcacgcggc gtgtcgcgat cgtcccagta acggttgagc agcgatccgt
    40801 ccggcatccg cacaacatgg cgataggcct gattagggat caacgattct gcaccgtcca
    40861 tccagaaggc atattccatt ttcaggtggt cgagatagcg gcgcgcaccg cgtacaccat
    40921 cttcttcaaa caactccacc atcagcgcaa aaaccggtgg ttgcgagcgg ctcaaataat
    40981 aggtgcggtt gccgttgggg atgtgaccgt agttttcgat catccaggcg aagttatcgg
    41041 ccatgcattt cagcaaatct tcccgaccac tttccgccag ccccagcatg gtgaaatagg
    41101 aatcccaata gtaggtttcg ctaaaacggc cgcccgggac aatatatgac tgcggtagcg
    41161 ccagcagaga agaccacgga atgtgatcct gtggttcgcg ggttagcacc ggccacagct
    41221 ggtcgatatg ctctttcagg gaattttgcg ggtccgatac atactcgctg gagtagacct
    41281 ccggcagcca gaagtgattt tcaacaaact tgcgcaagtc aaaatcacga tgacggcgca
    41341 ctttacggta gcggattaaa atatccagcg ggtccatttt gggtgcgcag tcgggaaagg
    41401 ttttactgtc ggggaaaatt ttcgccgact gaacatgctc gaacagttcg agatagcgat
    41461 cggcaggcgt cagcgcatca gaggcgggca gcccttcaat catctcgggt tccggttctg
    41521 cctcgatcat ctcatccagt tttaattcat acgggtccag ttcatagcag agatcgactt
    41581 cgatcatcag ttcgtctgga ttagggtttt gaattttctg attgagcata aggatccaac
    41641 ctccgaaatt cgtcgctaat tatcggcttt tctttaaggg tagtcattcg ctccgcctgg
    41701 tgggcgacaa agcgtgcggt ggatcacgtt tattaacttg tcaaaaaata taacgcacta
    41761 atatcaatgg aatttataaa aatacggatt tggtgttgtt tttcattttc agaacattca
    41821 atcctgctct cgccgcaatc atgatatgcc attcctgatc tgggtaatag gcacaggcta
    41881 tcttattgat agtttatatt catgtaattg attgattcct atcaaaaagc gtatctccct
    41941 gttttttatg atggctttgt ctcgcagaga cgtttgcagc attctggaaa attttctggg
    42001 tcacaataac tcaccaccgt gagggaagaa taatgaaaat ggtctcacgt attaccgcga
    42061 tcggcctggc tggcgtcgcg atttgctatt tagggttatc tggttatgtg tggtaccacg
    42121 ataataaacg cagtaaacag gccgatgttc aggcatctgc tgtcagtgaa aataataagg
    42181 ttttaggctt tctccgcgaa aaaggatgcg actattgcca cacgccttcg gcagaattac
    42241 ccgcctatta ttatattcct ggcgcgaaac agttgatgga ttacgacatt aagcttggat
    42301 ataaatcttt taacctcgag gccgtgcgtg cggcactgct ggctgataaa cccgtttcgc
    42361 aaagcgatct gaataagatt gaatgggtga tgcagtatga aactatgcca ccaacgcgtt
    42421 ataccgcgct acactgggcg ggtaaggtga gtgatgaaga gcgggcggaa atactggcct
    42481 ggattgcaaa acagcgcgcg gaatattacg ccagcaatga tactgctccg gagcatcgca
    42541 atgaaccggt gcagcccatc ccgcaaaaac tgcctaccga tgcgcaaaaa gtggcgttgg
    42601 gctttgcgct gtatcacgat ccccgtttat cggctgatag caccatttca tgcgctcatt
    42661 gccatgcgtt gaatgcgggg ggcgtcgatg gcagaaaaac atcgattggt gttggtggcg
    42721 cagttgggcc gattaacgcg ccgacggtat ttaactcagt atttaacgtt gagcagttct
    42781 gggatggtcg tgcggcaaca ttgcaggatc aggcaggtgg accgccgttg aacccgattg
    42841 aaatggcgtc gaaatcctgg gacgaaatta ttgctaagct ggaaaaagat ccgcagctta
    42901 aagcgcagtt cctcgaagtt tatccgcaag gtttcagtgg cgaaaatatt actgatgcca
    42961 ttgctgaatt tgagaaaaca ttaattacgc cggattcccc atttgataaa tggttgcgtg
    43021 gagatgaaaa tgcgctgacg gcgcaacaga aaaaaggcta tcaattattt aaagataata
    43081 aatgtgcaac ttgtcatggt ggtattattc tcggcggacg ttcctttgaa ccgttggggc
    43141 tgaaaaaaga ctttaacttt ggggaaatta cggcggcgga tattggtcgt atgaatgtga
    43201 ctaaagaaga gcgtgataaa ttgcgccaga aagtacccgg tttacgtaac gttgctttaa
    43261 cggcaccgta cttccatcgc ggtgacgtgc cgacgctgga cggggcggtg aaactgatgc
    43321 tgcgctatca ggtaggcaaa gagctgccgc aggaggatgt ggatgatatc gtagctttcc
    43381 tgcacagtct gaacggggtg tacacgccgt atatgcagga taaacaataa ctaatttgat
    43441 cgcccgaaca ggaatgtttg ggcgattttt attacgataa taaagtctgt ttttaatatt
    43501 atcatgttaa atgtttatat tataaaaagt cgtttttctg cttaggattt tgttatttaa
    43561 attaagcctg taatgccttg cttccattgc ggataaatcc tactttttta ttgccttcaa
    43621 ataaatttaa ggagttcgaa atggaccaga agctgttaac ggatttccgc tcagaactac
    43681 tcgattcacg ttttggcgca aaggccattt ctactatcgc ggagtcaaaa cgatttccgc
    43741 tgcacgaaat gcgcgatgat gtcgcatttc agattatcaa tgatgaatta tatcttgatg
    43801 gcaacgctcg tcagaacttg gccactttct gccagacctg ggacgacgaa aacgtccata
    43861 aattgatgga tttgtcgatc aa
//
LOCUS       DAJTOR010000009        42199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTOR010000009 DAJTOR010000000
VERSION     DAJTOR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.009"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..597)
                     /gene="yeeE"
                     /locus_tag="OO249_000483"
                     /pseudo
     CDS             complement(1..597)
                     /gene="yeeE"
                     /locus_tag="OO249_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
     gene            complement(776..2134)
                     /gene="plaP"
                     /locus_tag="OO249_000484"
     CDS             complement(776..2134)
                     /gene="plaP"
                     /locus_tag="OO249_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5634895.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYRVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(2124..2186)
                     /gene="yoeI"
                     /locus_tag="OO249_000485"
     CDS             complement(2124..2186)
                     /gene="yoeI"
                     /locus_tag="OO249_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5634896.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(2401..3330)
                     /locus_tag="OO249_000486"
     CDS             complement(2401..3330)
                     /locus_tag="OO249_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5634897.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQHFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(3376..4200)
                     /locus_tag="OO249_000487"
     CDS             complement(3376..4200)
                     /locus_tag="OO249_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5634898.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGLVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(4283..4537)
                     /gene="yoeB"
                     /locus_tag="OO249_000488"
                     /pseudo
     CDS             complement(4283..4537)
                     /gene="yoeB"
                     /locus_tag="OO249_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
     gene            complement(4534..4785)
                     /gene="yefM"
                     /locus_tag="OO249_000489"
     CDS             complement(4534..4785)
                     /gene="yefM"
                     /locus_tag="OO249_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5634899.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            5068..5118
                     /gene="hisL"
                     /locus_tag="OO249_000490"
     CDS             5068..5118
                     /gene="hisL"
                     /locus_tag="OO249_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5634900.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    5094..5217
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            5264..6163
                     /gene="hisG"
                     /locus_tag="OO249_000491"
     CDS             5264..6163
                     /gene="hisG"
                     /locus_tag="OO249_000491"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5634901.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            6169..7473
                     /gene="hisD"
                     /locus_tag="OO249_000492"
     CDS             6169..7473
                     /gene="hisD"
                     /locus_tag="OO249_000492"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5634902.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSLESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            7470..8540
                     /gene="hisC"
                     /locus_tag="OO249_000493"
     CDS             7470..8540
                     /gene="hisC"
                     /locus_tag="OO249_000493"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5634903.1"
                     /translation="MSIVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYGVSAETIGVECRTVPTLDNWQLDLQGISDKLEGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     ECLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            8540..9607
                     /gene="hisB"
                     /locus_tag="OO249_000494"
     CDS             8540..9607
                     /gene="hisB"
                     /locus_tag="OO249_000494"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5634904.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAIDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9607..10197
                     /gene="hisH"
                     /locus_tag="OO249_000495"
     CDS             9607..10197
                     /gene="hisH"
                     /locus_tag="OO249_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5634905.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            10197..10934
                     /gene="hisA"
                     /locus_tag="OO249_000496"
     CDS             10197..10934
                     /gene="hisA"
                     /locus_tag="OO249_000496"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5634906.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10916..11692
                     /gene="hisF"
                     /locus_tag="OO249_000497"
     CDS             10916..11692
                     /gene="hisF"
                     /locus_tag="OO249_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5634907.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEVVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11686..12297
                     /locus_tag="OO249_000498"
     CDS             11686..12297
                     /locus_tag="OO249_000498"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5634908.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVAFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLLLVNPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(12393..13370)
                     /gene="wzzB"
                     /locus_tag="OO249_000499"
     CDS             complement(12393..13370)
                     /gene="wzzB"
                     /locus_tag="OO249_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5634909.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(13517..13609)
                     /locus_tag="OO249_000500"
                     /pseudo
     CDS             complement(13517..13609)
                     /locus_tag="OO249_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            13779..14783
                     /locus_tag="OO249_000501"
     CDS             13779..14783
                     /locus_tag="OO249_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HCR5634910.1"
                     /translation="MKFLVTGAAGFIGFYLSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFERVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKNPQWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            complement(14849..16015)
                     /gene="ugd"
                     /locus_tag="OO249_000502"
     CDS             complement(14849..16015)
                     /gene="ugd"
                     /locus_tag="OO249_000502"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR5634911.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKRQSP
                     LVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSERAKRFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKNGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(16264..17670)
                     /gene="gndA"
                     /locus_tag="OO249_000503"
     CDS             complement(16264..17670)
                     /gene="gndA"
                     /locus_tag="OO249_000503"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5634912.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIT
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFHDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELIAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEEGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDNAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(17775..18608)
                     /locus_tag="OO249_000504"
     CDS             complement(17775..18608)
                     /locus_tag="OO249_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5634913.1"
                     /translation="MNHRLEKFSVLISIYKNDLPQFFEVALRSIFHDQTLKPDQIVIV
                     ADGELHQTHIDIINSFIDDVGNKIVTFVPLPRNVGLANALNEGLKACRNELVARMDAD
                     DISLPHRFEKQISFMINNSEIDVCGSFIDEIETVTEEFISTRKVPLEHREIVKFARKR
                     SAVSHPSVIFRKNTVLAVGGYPPFRKSQDFALWSLLIVHNARFANLPDILLKMRTGRN
                     LMARRGLSYLLYEYKVLYYQYKIGFIRKNELISNAMLRTFFRIMPSKLKELMYSIVRN
                     R"
     gene            complement(18605..19474)
                     /locus_tag="OO249_000505"
     CDS             complement(18605..19474)
                     /locus_tag="OO249_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5634914.1"
                     /translation="MDTVYAVLVAYNPEHNDLKNAVELLLRQVTKVVVCNNSTNGYKY
                     AENSSGDVKIFNFNDNLGIAEAQSIGMKWAFENGADFILQMDQDSIPDPKMVEQLLTC
                     YKKLLKQNVNVGLVGSQDFDKVTGELNKARVKKGKPLTEVYYEVDSTLSSGSLIPKNS
                     WLIVGGMKDELFIDAVDHEYCWRLRAAGFKVIRNKNALLAHRLGDGRFKILNILSVGL
                     PSPFRHYYATRNIFLLLNKNYVPIYWKISSLVKLIGKVFLYPIFLPNGNKRLYFFLKG
                     INDGLMGRSGKMK"
     gene            complement(19491..20678)
                     /gene="wzy"
                     /locus_tag="OO249_000506"
     CDS             complement(19491..20678)
                     /gene="wzy"
                     /locus_tag="OO249_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen polymerase"
                     /protein_id="HCR5634915.1"
                     /translation="MTSDFYNSKDKSLSVLLFFGFIFFLTRSFPFIQYSWIMEGFLCL
                     CIMSFTKKIANGIYHYPVILIFLLALFINFIYSYIKGNDIAIIIRFYIIILFILCAYF
                     CSYGTISIVKIFLYLMVLQAVIISIISIYMTKTYGIGDYSALRHYFLENDYGDVYTYG
                     SGFYRVQIKGNALIPFAFMLHIVIKDYFYYRFKNTITVILAIGTIVAGNFAYFVSICL
                     FFMYIILCSKSNSRYAKLRKIIFGVFLTVILPFFITYSIELIIMKSNGADSSLGVRWD
                     QFTVLINDLTESVSNFVIGSGLGNVIKIQTPIRDYSAYIYYELQSVYFLNQLGVILFT
                     LFLLINLLLTIKIIKYSELCVLYFLYVSYAITNPYILDSNHVAVIIVLVTLSNVLKKM
                     KAK"
     gene            complement(20734..21966)
                     /gene="wzx"
                     /locus_tag="OO249_000507"
     CDS             complement(20734..21966)
                     /gene="wzx"
                     /locus_tag="OO249_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen flippase"
                     /protein_id="HCR5634916.1"
                     /translation="MSIVKNTLWNISGYIIPSLIAIPALGILSRILGTEQFGLFTLAI
                     ALVGYASIFDAGLTRAVIREVSIYKNVHKELRAIISTSTVILTILGLIGGSVLFLSSN
                     VIVKLLNINANHVVESVKAIYIISATIPLYLLNQVWLGIFEGMEKFRKVNLIKSINNS
                     FVAGLPVIFCFFHGGLLSAIYGLVMARVLSLIVTFIFSRKLIISSGLSVKIVTVKRLI
                     GFGSWITVSNIISPIMTYMDRFILSHIVGADKVSFYTAPSEGIQRLTILPSALSRAIF
                     PRLSSELQSVKQTKILSYFIMVIGILPIVMLIIILSDFIMSAWMGPTYHGTPGIVLKI
                     LAIGFFFNCIAQIPFVSVQASGRSKITAIIHLLEVIPYLCILYIFIYHWGIVGAAIAW
                     SVRTSLDFLILLLIDTKY"
     gene            complement(21963..22520)
                     /gene="rfbC"
                     /locus_tag="OO249_000508"
     CDS             complement(21963..22520)
                     /gene="rfbC"
                     /locus_tag="OO249_000508"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5634917.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPDSDRGIVWNDPILSIKWPTI
                     EHNNYILSIKDARAKELHNMKELFL"
     gene            complement(22525..23403)
                     /gene="rfbA"
                     /locus_tag="OO249_000509"
     CDS             complement(22525..23403)
                     /gene="rfbA"
                     /locus_tag="OO249_000509"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5634918.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(23461..24360)
                     /gene="rfbD"
                     /locus_tag="OO249_000510"
     CDS             complement(23461..24360)
                     /gene="rfbD"
                     /locus_tag="OO249_000510"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5634919.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKEANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAAKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELITTTAI"
     gene            complement(24360..25445)
                     /gene="rfbB"
                     /locus_tag="OO249_000511"
     CDS             complement(24360..25445)
                     /gene="rfbB"
                     /locus_tag="OO249_000511"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5634920.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKTRFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTLEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            complement(25818..26711)
                     /gene="galF"
                     /locus_tag="OO249_000512"
     CDS             complement(25818..26711)
                     /gene="galF"
                     /locus_tag="OO249_000512"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5634921.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(26943..27938)
                     /locus_tag="OO249_000513"
     CDS             complement(26943..27938)
                     /locus_tag="OO249_000513"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HCR5634922.1"
                     /translation="MNNNVLLIGASGFVGTRLLETAIADFNIKNLDKRQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(28096..29490)
                     /gene="wcaM"
                     /locus_tag="OO249_000514"
     CDS             complement(28096..29490)
                     /gene="wcaM"
                     /locus_tag="OO249_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5634923.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGHTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMSNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLVYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(29501..30721)
                     /gene="wcaL"
                     /locus_tag="OO249_000515"
     CDS             complement(29501..30721)
                     /gene="wcaL"
                     /locus_tag="OO249_000515"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5634924.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEILALQKGDTQN
                     THAAWTKYNLAARTHWLQDEPTGKIAKLRHRASQTLRGIYRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLGTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(30718..31998)
                     /gene="wcaK"
                     /locus_tag="OO249_000516"
     CDS             complement(30718..31998)
                     /gene="wcaK"
                     /locus_tag="OO249_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5634925.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMMALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(32274..33752)
                     /gene="wzxC"
                     /locus_tag="OO249_000517"
     CDS             complement(32274..33752)
                     /gene="wzxC"
                     /locus_tag="OO249_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5634926.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCLAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPQGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKPLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQFLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(33754..35094)
                     /gene="wcaJ"
                     /locus_tag="OO249_000518"
     CDS             complement(33754..35094)
                     /gene="wcaJ"
                     /locus_tag="OO249_000518"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5634927.1"
                     /translation="MVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQML
                     GGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTS
                     IGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLMESFRNQPWLGFEVVGVYH
                     DPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGARVKKLVHQLADTTCSVLL
                     IPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRAEDIVLATLILLLISPVLC
                     CIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTKV
                     GNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKVKPCITG
                     WAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY"
     gene            complement(35199..36569)
                     /gene="cpsG"
                     /locus_tag="OO249_000519"
                     /gene_synonym="manB"
     CDS             complement(35199..36569)
                     /gene="cpsG"
                     /locus_tag="OO249_000519"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5634928.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTSVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSRDALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(36762..38198)
                     /gene="cpsB"
                     /locus_tag="OO249_000520"
     CDS             complement(36762..38198)
                     /gene="cpsB"
                     /locus_tag="OO249_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5634929.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSSVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(38201..39424)
                     /gene="wcaI"
                     /locus_tag="OO249_000521"
                     /pseudo
     CDS             complement(38201..39424)
                     /gene="wcaI"
                     /locus_tag="OO249_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(39421..39900)
                     /gene="gmm"
                     /locus_tag="OO249_000522"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(39421..39900)
                     /gene="gmm"
                     /locus_tag="OO249_000522"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5634930.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLPGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(39903..40868)
                     /gene="fcl"
                     /locus_tag="OO249_000523"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(39903..40868)
                     /gene="fcl"
                     /locus_tag="OO249_000523"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5634931.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQSTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(40871..41992)
                     /gene="gmd"
                     /locus_tag="OO249_000524"
     CDS             complement(40871..41992)
                     /gene="gmd"
                     /locus_tag="OO249_000524"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5634932.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYCESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIVTGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLDDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(42018..>42199)
                     /locus_tag="OO249_000525"
     CDS             complement(42018..>42199)
                     /locus_tag="OO249_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5634933.1"
                     /translation="PIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPVNVVC
                     RGNPAVVIRERVETE"
BASE COUNT        11050 a        10723 c         9740 g        10686 t
ORIGIN      
        1 atgccagcgt ttttcaaaca gaatatgagc gatcccggtt cggcgcggcg gtaaggtcgc
       61 gactttgagt tttggcttct tcaactcttt catcaccacc cagagcgtta tcaccagcaa
      121 aacggcaacc aacggccaca cagacaaatt aaaagtctca gcaatagagt tatgttcagt
      181 actgtagtgc tgcaaggttt gatttaaacc actggcatgt ggagaacgca tcaccgcact
      241 catcaccata taagtgaaaa gcgcgatcca actgccgatc aatccctcac ccgcgcgata
      301 ccaggtcccg gtggcacatc cacccgccag aacaattccc agcccgaaga tatacccacc
      361 tataacagta ccaggccacg ggaacgcccc ggcttcgtaa gtcagtaggc ccgcctaaat
      421 taacgcaaaa acccccacac tttgtaccga aattgcaatc agcaaggcgt aaaacatgcg
      481 attatttttc gcgatataca tatcgcgaaa accaccagtc aggcaaaaac gcccacgctg
      541 catgacaaat cccagcagag caccacaaat tagcccgctt aatatcattg aaaacataaa
      601 ttaattaacc agatgaatgt taatgaggaa attattcatg actggtggaa tgcagaccaa
      661 taaccaaatt ctctaaatta gaacaaatgg ttcttaatga ggatttcgat aaaaaagaat
      721 aaaaaaaccg gagccaaata ttcattcgac tccggttgat tattagaaaa gatgattatg
      781 ctacgtcttc gtactgcgga accgggttac ggaagctctt ggtgacgcaa gccaggtaaa
      841 tcagaccgat agcagcccag atcagaccca gaaccattga gctttcttcc aggttaaccc
      901 acagcgcacc aacggtcagc gcaccacaca tcggcaggaa cagatactgg aagtgatctt
      961 tcagcgtctt attacgcttc tcacggatcc agaactgcga gatgaccgac aggttaacga
     1021 aggtgaacgc caccagcgca ccaaagttaa tcagcgccgt tgccattacc aggtcgaagt
     1081 tgattgccag cagggcaatc gcgccaacca ggatgatgtt catcgccgga gtacgccatt
     1141 tcgggtgtac ataaccgaag aagcttttcg ggaatacgcc gtcacgaccc attacgtaca
     1201 tcagacgcgc tacgcctgca tgcgccgcca taccggacgc cagtacggta atggtggaga
     1261 agatcagcgc gccaacctgg aacgctttac ccgcaacgta cagcatgatt tcaggctgtg
     1321 acgcatccgg atctttaaag cgagagatat ccgggaagta cagctgcagg aagtaagttg
     1381 caaagatgaa gatcatgccg ccgatcagcg cggtcaggaa aatcgcacgc gggatcacgc
     1441 gctctgcatc tttggtttct tccgacaggt tgctgatgcc gtcaaagccg gtaaaggaga
     1501 agcacaggat tgtcgccccg gtaatcatcg ggataacatg cgcatcgcca gaccagaatg
     1561 gacgagtgct cgccagcgta ccagcgcctt caccttcaaa tacgcgataa acaaccatgc
     1621 ccagaatcac cgcgatcagc accacctgca acacgacgat tacagtgttg aagttcgcta
     1681 cggatttcag actacgcagg ttaaaggcgg tcatgaaggc caccaacgcc accacaaaca
     1741 tccacgatgg gatggaaggc accagagctt caaaatagat tttcgccagc agaatgttga
     1801 tcatcggcgc gaacagatag tcgagcagag aagaccaacc caccataaag ccgacagtcg
     1861 ggctaatgga tttctgggcg taagtgtatg cagagccagc agaaggatag cggcgaacca
     1921 gcttcccgta gctcagagcc gtaaacagga tcgcaatcaa tgcgaacgca taggctgtcg
     1981 gcacatgacc atccgtgagg cctgaaacga taccaaaagt atcaaacagc gtcatcggct
     2041 gcatataggc aagacccatc attacaaccg gaactaacgt aagcgtttta cgcaattcca
     2101 cgcgagaggt gtttggagta acgttatgcg acatggtcat tctcctttac ggtgataacc
     2161 gtcgcgtaag caaaaaattg ccccattttt ttggattcct cagcgacaac aactgtcgat
     2221 ttttagtaaa tatctatccg gtacgaagcc cggcctcttg gtatgaatga ttggtttgaa
     2281 gcaaaaaata accgacgctg atgaaacgtc ggtttttagt cattttttga cagcggcgca
     2341 ttgtgcctaa atggggggga aatgacaaga gaatgagagg cttgtcataa taatttttct
     2401 ttaaatggct gattttccgt catcagattt tgcataaaca ccggcgatcg cactatttgc
     2461 taaaatttca tcccgccacc agccactggc aagtcctgcg gtctgttcat tccagccaat
     2521 ccagacgggt tcaaagagag tttgggcgac cactttcttc tcgattaacg cgccactatc
     2581 gagaaaatgt tgcgccagat aacggggtaa atagccgcag ccgaggccgc taatttgcag
     2641 ctccagcttg gttttaaaat cgaaaacggt aatggcttct tgttcgtcga gaagttgtga
     2701 ggccgtagaa gcgcctgcct gcgcagtatc ccccaccaca atcgcccgat atcgcttaat
     2761 aatacgacga tttaacggct cttcttccag ggctaacgga tgatgaggcg cgaccgcaaa
     2821 gacttgttcc agatcgccca accgtgaaaa accaaactca ctggaagatg gtggctcatg
     2881 catcgcgcca acaataatat ccgctcgccc ctgagtcagg gcatcccatg aaccacccag
     2941 tacgccattg ataaacttca ggcgcgtcac gctatgatgt tgataaaaag cttcaatcag
     3001 tggcgctagc agagaaaaag gaaacgtgtc gtccacgcca atcaccagct cgttttccca
     3061 gccttcatgc agtttgatcg cctgcttttc cagttcccgc acggtatgca gaacttcccg
     3121 ccctttctct aataacattt ttccggtgcg ggtgaattta gcgcggtgac cgctacgatc
     3181 aagcaattga atattaaggt cgctttccag cttgtgaacg gtgtaactca gagcggacgg
     3241 cgtcttgtag agtttggccg acgccgccgc aaaactgcct tctttttcta aggcatcgag
     3301 gatcatcaac acatccagca gtggtttcat acacgccccc ttgcagtgta tgcggcgtcc
     3361 tgctggcgtg atcgtttact ccagcggcat taccagcgga tcgggatact ggtattcaaa
     3421 tcccagttca ttacaaatcc gactgccatc aataatctta cctttgccgc tgtccagact
     3481 atttctgaac tgcggaggtt ccagccccag taaacgggcc atctgcggat agaaaacatt
     3541 acgcgcaggg tgagctggcg cacatatatt atagatgtgt ccgcctttag gtgcctgtaa
     3601 caacagagtg atagcgccaa taacatcttc taaatggact aaattaacac catgttcacc
     3661 atcaggcgcg gtttttccgg caaagaagcg tccgggatga cgccccggtc cgaccaggcc
     3721 cgcaagacgc agaatatcga ccgaagtacc gggtaaattg tgcagccagt cttcgagttc
     3781 ttctaatact agtccactgt tggttaccgg attacgcggg gtagtttctt tcaccatgcc
     3841 ttgcgcatcg ccatagacag atgttgagct ggtaaaaata attcggggaa tacgatgggc
     3901 cagcgcgcta tccactaact cttgtaccgc ttgtaaatag aactcatcgc cggagccgct
     3961 acgacgtgcc ggaagcgtaa tgaccagtgc atcggcatcc atcagggcat ccagatcgtc
     4021 agaatcgcaa actaactcag gctccatgcg aagcagatag ctatcaatgc cactcattcg
     4081 ggccgcttcg acaccatctt gtgtggtttt actcccggtg acttgccagc ctcgcgctga
     4141 aagtgacatc gccagcggca tgcctaacca ccctaacccg acaattgcga cctttttcat
     4201 ccgttatctc ctgatacacc ttagatctat aaggctacgc tagcgtatca aaactgacaa
     4261 ttcattctat gaatgaatct gttcaataat gataacgaca tgctgcaatg agcagtgaat
     4321 cgtcggtaac cgcgtatacc agacggtgct cctctgtaat gcgtcgggac tagaaacctg
     4381 acaaattatg tttcaggggt tctggcttcc ccttaccttc aaatggcgtt ctgcgggtat
     4441 ctttgataag ttcattgatc tttttaacaa ttcgcttatc tgtttcctgc cagtacagat
     4501 aatcatccca tgattcctca gaccagatta gtttcactca ataatgtcct tttctgttcc
     4561 tttgcctgat ttcaggctat cgattgagtc catcaatctc cgggcgttag cgggggaacg
     4621 cagtagataa gccgtctctt ccagcgagtt gtattcttcg agtgacatca gaacacaagc
     4681 ctctccattc tgacgagtaa taaggatcgg ggcatgatct tcaacggctt tcatcattgt
     4741 tgccgacaaa ttctgacgcg cttcgctgta gctaattgta cgcatgtcaa tctcctcttt
     4801 tgtacagttc attgtacaat gatgggcgtt aattaactat ttattaatta gtttgtagat
     4861 caaggtattg tcagtgagac gaaaatccag gcttcgctat ttttggtgcc gtcagcttag
     4921 aggacagtcc tcttagcccc ctcctttccc cgctcattca ttaaacaaat ccatcgccat
     4981 agaatatata aaaaagccct tgctttctaa cgtgaaagtg gtttaggtta aaagacatca
     5041 gttgaataaa cattcacaga gacttttatg acacgcgttc aatttaaaca ccaccatcat
     5101 caccatcatc ctgactagtc tttcaggcga tgtgtgctgg aagacattca gatcttccag
     5161 tggtgcatga acgcatgaga aagcccccgg aagatcacct tccgggggct tttttattgc
     5221 gcggttgata acggttcaga caggtttaaa gaggaataag aaaatgacag acaacactcg
     5281 tttacgcata gctatgcaga aatccggccg tttaagtgat gactcacgcg aattgctggc
     5341 gcgctgtggc attaaaatca atcttcacac ccagcgcctg atcgcgatgg cagaaaacat
     5401 gccgattgat attctgcgcg tgcgtgacga cgacattccc ggtctggtaa tggacggcgt
     5461 ggttgacctc gggattatcg gcgaaaacgt gctggaagaa gagctgctta accgccgcgc
     5521 ccagggtgaa gatccacgct actttaccct gcgtcgtctg gatttcggcg gctgccgact
     5581 ttcactggca acgccggttg atgaagcctg ggatggcccg ctctccttaa acggtaaacg
     5641 tatcgccacc tcttatcctc acctgctcaa gcgttatctc gaccagaaag gtatctcttt
     5701 taaatcctgc ttactgaacg gttctgttga agtcgccccg cgtgccggac tggcggatgc
     5761 gatttgcgat ctggtttcca ccggtgccac gctggaagct aacggcctgc gcgaagtcga
     5821 agttatctat cgctcgaaag cctgcctgat acaacgcgat ggcgaaatgg aagaatccaa
     5881 acagcaactg atcgacaaac tgctgacccg tattcagggt gttatccagg cgcgtgaatc
     5941 aaaatacatc atgatgcacg caccgaccga acgtctggat gaagtcatcg ccctgctgcc
     6001 aggtgccgaa cgcccaacta ttctaccgct ggcgggtgat caacagcgcg tagcgatgca
     6061 catggtcagt agcgaaaccc tgttctggga aacgatggaa aaactgaaag cgctgggtgc
     6121 cagttcaatc ctggtcctgc cgattgagaa gatgatggag tgatcgccat gagctttaac
     6181 acaatcattg actggaatag ctgtactgcg gagcaacaac gccagctgtt aatgcgcccg
     6241 gcgatctccg cttctgaaag cattacccgc actgttaacg atattctcga taacgtgaaa
     6301 gcacgcggcg atgaggccct gcgggaatac agcgcgaagt ttgataaaac cacggttacc
     6361 gcactgaagg tgtctgctga ggaaattgcc gccgctagcg aacgcctgag cgacgagcta
     6421 aaacaggcga tggcggtggc agtgaagaat attgaaacct tccacactgc gcaaaaactg
     6481 ccgccggtag atgtagaaac gcagccaggc gtgcgttgcc agcaggtcac gcgcccggtc
     6541 gcttcagttg ggttgtatat tcctggcggc tccgccccgc tcttctcaac ggtattaatg
     6601 ctggcgactc cggcgcgtat tgcgggctgt aaaaaagtgg tgctgtgctc accgccgccg
     6661 attgccgatg agatccttta tgcggcgcag ctgtgcggtg tgcaggacgt gtttaacgtc
     6721 ggcggcgcac aggccattgc cgcactggcg tttggtacgg aatctgtgcc gaaagtggac
     6781 aaaatcttcg ggccgggcaa cgcctttgtc accgaagcga aacgtcaggt gagccagcgt
     6841 ctggacggtg cggcgatcga tatgcccgca ggcccgtcgg aagtgttggt cattgccgac
     6901 agtggcgcaa cgccggattt cgtggcttct gatttgcttt ctcaggctga acacggcccg
     6961 gactcacagg tgattttact gacgcccgct gctgatatgg cgcgtcgcgt tgccgaggct
     7021 gtcgaacgcc agctggcaga actgccgcgt gctgaaaccg cccgtcaggc actgaacgcc
     7081 agccgcctga tcgtgactaa agatttagcg cagtgcgtag agatctccaa ccagtacggc
     7141 ccggagcacc tgatcatcca gacccgcaac gcccgcgatc tggtcgatgg cattaccagt
     7201 gccggttcgg tatttcttgg tgactggtca ctggaatcgg caggtgatta cgcctccggc
     7261 accaaccacg ttctgccgac ttacggttac accgccacct gttccagcct cgggctggca
     7321 gatttccaga agcgcatgac cgtgcaggaa ctgtcgaaag aaggtttctc cgcgctggct
     7381 tcgaccattg aaacattggc cgccgccgag cgcctgaccg cccataaaaa tgccgttact
     7441 ttacgtgtta acgcccttaa ggagcaagca tgagcatcgt gactattacc gatttagcgc
     7501 gtgaaaacgt gcgcaacctg acgccgtatc agtcggcgcg tcgtctgggc ggtaacggcg
     7561 atgtctggct gaacgccaac gaatatccca cagccgtgga atttcagctt actcagcaaa
     7621 cgctcaaccg ctacccggaa tgtcagccga aagcggtgat tgaaaattac gcgcaatatg
     7681 caggcgtaaa accggaacag gtgctggtca gccgtggcgc ggacgaaggt attgaactac
     7741 tgattcgcgc tttttgcgaa ccgggtaaag acgccatcct ctactgcccg ccaacgtacg
     7801 gcatgtacgg cgtcagcgcc gaaaccattg gcgtcgagtg ccgcacagtg ccgacgctgg
     7861 acaactggca actggattta cagggcattt ccgacaagct ggaaggcgta aaagtggtct
     7921 atgtttgcag ccccaacaac ccgaccgggc aactgatcaa tccgcaggat tttcgcactc
     7981 tgctggagtt aacccgcggt aaagcgattg tggttgccga tgaagcctat atcgagtttt
     8041 gcccgcaggc atcgctggct ggctggctgg cggaatatcc gcacctggct attttgcgca
     8101 cactgtcgaa agcttttgct ctggcgggcc ttcgttgcgg atttacgctg gcaaacgaag
     8161 aagtcatcaa cctgctgatg aaagtgattg ccccctaccc gctctcgacg ccggttgccg
     8221 acattgcggc ccaggcgtta agcccgcagg ggatcgtcgc catgcgtgag cgggtggcgc
     8281 aaattattgc agaacgcgaa tgcctgattg ccgcactgaa agagatcccc tgcgtggagc
     8341 aggttttcga ctctgaaacc aactacattc tggcgcgctt taaagcctcc agtgcagtgt
     8401 ttaaatcttt gtgggatcag ggcattatct tacgtgatca gaataaacaa ccctctttaa
     8461 gcggctgcct gcgaattacc gtcggaaccc gtgaagaaag ccagcgcgtc attgacgcct
     8521 tacgtgcgga gcaagtttaa tgagtcagaa gtatcttttt atcgatcgcg atggaaccct
     8581 gattagcgaa ccgccgagtg attttcaggt ggatcgtttt gacaaactcg cctttgaacc
     8641 gggcgtgatc ccggaactgc tgaagctgca aaaagcgggc tacaaactgg tgatgatcac
     8701 taatcaggat ggtcttggaa cacaaagttt cccgcaggcg gattttgatg gcccgcacaa
     8761 cctgatgatg cagatcttca cctcgcaagg cgtgcagttt gatgaagtgc tgatttgccc
     8821 gcacctgccc gccgatgaat gcgactgccg taagccgaaa gtaaaactgg tggagcgtta
     8881 tctcgctgag caagcgatag atcgcgccaa cagttatgtg attggcgatc gcgcgaccga
     8941 tattcaacta gcagaaaaca tgggcattac tggtttacgc tacgaccgcg aaaccctgaa
     9001 ctggccgatg attggcgagc aacttaccaa acgagaccgt tacgcccatg tagtgcgcaa
     9061 caccaaagag acgcagattg acgttcaggt gtggctggat cgcgaaggtg gcagcaagat
     9121 taacaccggc gttggcttct ttgatcatat gctggatcag atcgccaccc acggcggttt
     9181 ccgcatggaa atcaacgtca aaggcgacct ctatatcgac gatcaccaca ccgtcgaaga
     9241 taccggcctg gcgctgggtg aagcgttaaa aattgccctc ggcgataaac gcggtatttg
     9301 tcgctttggt tttgtgctgc cgatggacga atgccttgcc cgctgcgcac tggatatctc
     9361 tggtcgcccg cacctggaat ataaagctga atttacctac cagcgcgtgg gcgatctcag
     9421 caccgagatg atcgagcact tcttccgttc gctctcttac accatgggcg tgaccctgca
     9481 cctgaaaacc aaaggtaaaa acgatcacca ccgtgtagag agcctgttca aagcttttgg
     9541 tcgcaccctg cgccaggcca tccgcgtgga aggcgatacc ctgccctcgt cgaaaggagt
     9601 gctgtaatga acgtggtgat ccttgatact ggctgcgcca acctgaactc ggtgaagtct
     9661 gccattgcgc gccacggtta tgaacccaaa gtcagccgtg acccggacgt cgtgttgctg
     9721 gccgataaac tatttttacc cggcgttggc accgcacaag cagcgatgga tcaggtgcgt
     9781 gagcgcgagc tgtttgatct catcaaagcc tgtacccaac cggtgctagg catctgctta
     9841 gggatgcaac tgctggggcg gcgcagcgaa gagagcaacg gcgtcgattt gctgggcatc
     9901 atcgacgaag acgtgccgaa aatgaccgac tttggtctac cgctgcccca tatgggctgg
     9961 aaccgcgttt acccgcaggc aggcaaccgc ctgtttcagg ggattgaaga cggcgcgtac
    10021 ttttactttg ttcacagcta cgcaatgcca gtcaatccgt ggaccatcgc ccagtgtaat
    10081 tacggcgaac cgttcaccgc ggcggtacaa aaagataact tctacggcgt gcagttccac
    10141 ccggagcgtt ctggtgccgc tggcgctaag ttgctgaaaa acttcctgga gatgtgatga
    10201 ttattccggc attagattta atcgacggca ccgtggtgcg tctccatcag ggcgattacg
    10261 gcaaacagcg cgattacggt aacgacccgc tgccgcgctt acaggattac gccacgcagg
    10321 gtgccgaagt gttgcatctg gtggatctga ccggggcaaa agatccagca aaacgtcaaa
    10381 tcccgttgat taaaaccctg gtcgcgggcg ttaacgttcc ggtgcaggtt ggcggcggcg
    10441 tgcgtagcga agaagacgtg gcggcgttac tggaagcggg cgttgcgcgc gtggtggtcg
    10501 gctccaccgc ggtgaaatca ccagaaatgg tgaaaggctg gtttgaacgt ttcggtgccg
    10561 atgccttagt gctggcgctg gatgtccgta ttgacgagca aggcaacaag caggtggcag
    10621 tcagcggctg gcaagagaac tcgggcgttt cactggaaca actggtggaa acctatctgc
    10681 ccgtcggcct gaaacatgtg ctgtgtaccg atatctcgcg cgacggcacg ctggcaggtt
    10741 ctaacgtctc tttatatgaa gaagtgtgcg ccagatatcc gcaggtggca tttcagtcat
    10801 ccggcggcat tggcgacatt aacgatgttg ctgccctgcg tggcactggc gtgcgcggcg
    10861 taatagttgg tcgggcatta ttggaaggta aattcaccgt gaaggaggcc atcgcatgct
    10921 ggcaaaacgc ataattccgt gtctcgacgt tcgtgatggt caggtggtga aaggcgtaca
    10981 gtttcgcaac catgaaatca ttggcgatat cgtgccgctg gcaaaacgct acgccgaaga
    11041 aggtgcagac gaactggtgt tctacgatat caccgcttcc agcgatggcc gtgtggtaga
    11101 taaaagctgg gtatctcgcg tggcggaagt gatcgacatt ccgttctgcg tggcgggtgg
    11161 gattaagtct ctggaagatg ccgcgaaaat tctttccttt ggcgcggata aaatttccat
    11221 caactcgcct gcgctggcgg atccgacgtt aattactcgc ctggcggatc gttttggcgt
    11281 gcagtgtatt gtggtgggta ttgatacctg gtacgacgct gaaaccggta aatatcatgt
    11341 gaatcaatat accggcgatg aaagccgcac ccgcgtcact cagtgggaaa cgctcgactg
    11401 ggtacaggaa gtgcaaaaac gcggtgccgg agaagtcgtc ctcaacatga tgaatcagga
    11461 cggcgtgcgt aacggttacg acctcgaaca actgaaaaaa gtgcgtgaag tttgccacgt
    11521 cccgctaatt gcctccggtg gcgcgggcac catggaacac ttcctcgaag ccttccgtga
    11581 tgccgacgtt gacggcgcgc tggcagcttc cgtattccac aaacaaataa tcaatattgg
    11641 tgaattaaaa gcgtacctgg caacacaggg cgtggagatc aggatatgtt aacagaacaa
    11701 caacgtcgcg aactggactg ggaaaaaacc gacggactta tgccggtgat tgtgcaacac
    11761 gcggtatccg gcgaagtgtt aatgctgggc tatatgaacc cggaagcctt agacaaaacc
    11821 atagaaagcg gcaaagtcgc cttcttctcg cgcactaaac agcgactgtg gaccaaaggc
    11881 gaaacgtcgg gcaatttcct caatgtggtg agtattgcac cggactgcga caacgacacg
    11941 ttactgttac tggtgaatcc tatcggacca acctgccata aaggcaccag cagctgcttc
    12001 ggcgacaccg ctcaccagtg gctgttcctg tatcaactgg aacaactgct cgccgaacgc
    12061 aaatctgccg acccggaaac ctcctacacc gccaaactgt atgccagcgg caccaaacgc
    12121 attgcgcaga aagtgggcga agaaggcgtg gaaaccgcac tggcagcgac agtacatgac
    12181 cgctttgagc tcaccaacga ggcgtctgat ttgatgtatc acctgctggt gctgttgcag
    12241 gatcaggatc tggacttaac gacggtgatt gagaacctgc gtaaacggca tcagtgagtt
    12301 gcggggtaag cggatgcgat attgttgtag catccggaaa aaaaacgggc aaggtgtcac
    12361 caccctgccc cttttcttta aaaccgaaaa gattacttcg cgttgtaatt acgcagagcg
    12421 ttacgcccca agacaatccc cgcgccaacc atgccgccca gcagcaccgc cagaatcaag
    12481 gtaattgcct ttttcgggct atcgcgacga ataggtaacg tcggtttcat tacatagcgg
    12541 taagcatgaa tatcaagttt atcaacgtca agattgtcga tatccagcag gttttgacga
    12601 gtctgatagt agtttggtga gaacaccaac ggacgagtcg cttcatgctt aatcatcgac
    12661 tccagcgctt cgctccccaa aaggaacaac gtatcttgtg tcacatcttc agtctgctga
    12721 acctgtggct ttgtcacctg cgcctgattc gcatactgca acgcttcctg aatctgacgg
    12781 atacgcagat ctttctgctc ctgcgcaact acttcctgag ttttcagaga atcctgcaag
    12841 tttttcatac gcagcgcgat gttgtctttc aggtcttttc ccagttcttc atttacctgg
    12901 tcgtcaactt gctggatata cttcgccagc tgcttctgag caccttcagg tgtttcacca
    12961 acataagaca ccgctaaagg taatgactga tttttgacgg taggctcaat ggtcagcttt
    13021 tctgcttctt cctggttatc cagcgtttcc gctaatgctg agaatgcagt gctgtaacgt
    13081 ccgataagtg aagcctgaat gtcggagact ttcggtacag ccgatccgta gatcacattc
    13141 atcgcgttgg tataaccagc gatttgcccc acatcaggtt gagtaacgat agctgtggaa
    13201 gtccactttt ctttggcaac ggccagatac cctatagcca gggcaatagc aacaatgaca
    13261 gagatgataa ttgtcatctt gccacgccac aactgcacca gtaaatcaat caaatcaatc
    13321 tgttccggat catggttttg cccagaaaca ttattatttt ctactctcat cattacccta
    13381 acggacgaaa taattttaag tcgccatagt ttagcgataa tcgccatgaa tgctatagga
    13441 taaatgataa aaataatgaa ttattacaaa gggaacataa gagaatcagg gcgaaaatcg
    13501 ccatgataac aggatgttag tcactgccaa agagatcgcg ggtgtagact ttgtctgcca
    13561 catccttaag ctcttctgcc atacggttgg agataatgac gtcggcttgt atagtcagct
    13621 tagaacagct ttgattgtaa aatgagcacg ttaccagaaa aaacagccaa gtttagaact
    13681 gataccgatt atcttttttt cttgtctaac ggtataattt aactttcagt tatgccagat
    13741 gaagattggc tatattctag cctgagcgag gattataaat gaaatttctg gttacgggag
    13801 ctgctggctt tatcggtttc tatctaagta aacggcttct tgcagctggt catcaggttg
    13861 taggcattga caacttaaat gattattacg atgtcaacct caaacaagca cgacttgatt
    13921 tactcaagca cgacaacttc agtttttata aaattgacct ggccgatcgt gagaaaatgg
    13981 cggcactgtt tgcagacgag cggttcgaac gcgtaataaa cctcgctgcg caagccggtg
    14041 tacgttactc tcttgaaaat cccaatgcat atgcagatgc aaacctgatt ggattcctga
    14101 acatactaga aggatgtcgc cataataatg ttcagcatct actttacgct tcctccagtt
    14161 ctgtttatgg catgaaccgc aagatgcctt tctctacaga tgactctgta gatcatcctg
    14221 tttcgcttta tgcagcaact aaaaaagcga atgaactcat ggcccataca tattctcatt
    14281 tgtatggctt accgaccaca gggctgcgtt tctttacggt ttatggtcca tggggacgtc
    14341 cggatatggc attatttaaa ttcactaaag ccatgctgga aggtaaaagc attgatgttt
    14401 acaacttcgg caaaatgaag cgtgacttta cttacattga tgatattgcc gaagctatta
    14461 ttcgcttaca ggatgttatt ccagaaaaaa acccacagtg ggctgtagaa acaggctcgc
    14521 ctgcaacaag ttcagcacca tatcgtgtct ataacattgg taatagttcg cctgtggagt
    14581 tgatggacta tatcaatgcg ttagaagagg ctctgggtat tgaagccaac aaaaatatga
    14641 tgcctctcca acccggtgat gtactggaaa ccagtgctga tacaaaagca ctgtatgacg
    14701 taataggatt caaacctgaa acgtcagtta aagaaggggt aaagaacttt gtagaatggt
    14761 atcgtaactt ctataaagtt tgattttaca aaaccataag aaaaggccct aatttattag
    14821 ggccttttct tagaatgaaa caaaataatt aatcattgcc aaacaagtcg cgcgtataaa
    14881 ctttatctgc tacatcagcc agatcggcag acatacggtt agaaataata acatcagctt
    14941 cttgtttgaa cgcatccaga tcacgtacca cgcgcgaccg gaaaaaatcg tcctctttca
    15001 tagctggctc ataaacgatt acaggcacac ctttcgcctt gattcgcttc ataataccct
    15061 gaatcgagga agcacgaaaa ttgtctgaac cattcttcat aatcaaacga tagacgccaa
    15121 caactttcgg tttacgtgca aggatagaat cggcaataaa atctttgcgc gtgcggttgg
    15181 cgtcaacaat tgccgagatc aggttattcg gcacagactg gtaatttgcc agtaactgct
    15241 tagtatcttt cggcagacaa taaccaccat aaccgaatga cgggttgttg tagtgattac
    15301 cgatacgcgg gtcaaggcat acgccctcaa taatctggcg tgaattaagt cccaggcttt
    15361 cagcataact atcaagttca ttgaaatacg ctacacgcat cgccagataa gtgttcgcaa
    15421 aaagtttaat cgcctcagcc tcggttgagt cagtaaacaa tgttggtatg tcttgcttaa
    15481 tggcgccttc ctgtaataac gcagcaaaac gtttagcgcg ttcagactgc tcgccaatca
    15541 caatgcgtga tgggtgtaag ttatcataaa gtgctttacc ttcacgcaaa aactcaggcg
    15601 aaaagatcac attttcaata ccaaaacgtt ctttaatgga ctctgtaaaa ccaacaggga
    15661 tagttgattt tataatcatt accgcgttgg gattaatttc tgtcacatca cgaatgaccg
    15721 cttccacgct tgaggtatta aaataatttg ttttcggatc ataatcggta ggtgtggcaa
    15781 taataacgta atcggcattt ttatacgcgt catacttatc tgtcgtagcg cggaaattga
    15841 gatctttagt cgccagatac tcttcaatct ccttatcaac aagcggtgac tgcctcttgt
    15901 taagcatgtc cactttggcc tgaacgatat ccagtgcaac cacttcgtgg ttttgcgcaa
    15961 tcagaatacc atttgaaaga ccaacataac ctgttcctga aattgttatt ttcattagct
    16021 ctgacttctt ccggttaaac atttagagtg gtcattaatc caccaccaca gcttcatcta
    16081 ctgcgggatt tttaacgctg atgtagatga actgtcaagg cagcgatcct gctgtgcggc
    16141 gctgtattat atcgcgtttt taactataaa ttataaaaaa aggccctaac ctgccgcttt
    16201 gtataataaa aaagcccgga gggtttctcc gggccttgct ttgattaatt gatttaaatc
    16261 agattaatcc agccattcgg tatggaacac accttcttta tcaatgcgct tataagtatg
    16321 cgcaccgaaa tagtcacgct gtgcctggat caggttcgca ggcagaacag cggcgcggta
    16381 gctgtcgtaa taggcaaccg cagcggcgaa ggtcggcacc gggataccgt tctgtactgc
    16441 gtaagcgacg acatcgcgca gcgcctgctg gtagtcatcg gcaatttgct tgaagtaagg
    16501 agccagcaac aggttagcga tctgcggatt ttcggcataa gcatcggtga ttttctgcag
    16561 gaactgcgca cggatgatgc agccagcacg gaaaatcttc gcgatttcac cgtagttcag
    16621 atcccagttg tactcttcag acgcagcgcg tagctgagag aagccctgag cgtaagaaac
    16681 gattttgccc agatacagcg cacggcgaac tttttcgatg aactcagcat tgtcgccagc
    16741 tggctgcgct tgcgggccag agagaacttt agatgcggca acacgctgct ctttcagaga
    16801 agagatataa cgtgcaaaca cagactcggt aatcagcgac agcggttcgc cgagatccag
    16861 cgcgctctgg ctggtccatt tgcccgtacc tttgtttgct gcttcatcca gaatcacatc
    16921 aaccaggtag ttaccctctt catctttttt ggtgaagata tctttggtga tgtcgatcag
    16981 gtagctgctc agttcaccgt tattccactc ggtaaaggtc tgcgccagtt cttcgttggt
    17041 gaggttcaag ccacctttaa gcagagaata ggcttcagca atcagctgca tatcaccgta
    17101 ttcaataccg ttgtgaacca tcttcacata atgacctgca ccatcggcac caatataggt
    17161 aacgcacggt tcgccgtctt cagccacagc ggcgattttg gtcaggatcg gcgcaatcag
    17221 ttcataagct tctttctgcc caccaggcat aatggaagga cctttcagcg caccttcttc
    17281 accaccggaa acaccggtac cgataaagtt aaagccttct gcagaaagct cacggttacg
    17341 acgaatggtg tcatggaaga aggtgttacc accatcaatg atgatgtcac ctttatcgag
    17401 gtatggcttg agggaatcaa tagcagcatc cgtgccagca cctgctttca ccattaacag
    17461 gatgcgacga ggcgtttcca gagattcaac aaattctttc accgtatagt aaggaaccag
    17521 tttcttgcct ggattttcgg taatcacttc ttcggtcttt tcacgggaac ggttgaaaat
    17581 agagacggta taaccacggc tttcgatatt gagcgcaagg ttgcgcccca tcactgccat
    17641 accgacgacg ccgatctgtt gctttgacat tgtttactcc tgtcaggata ccgctgggtg
    17701 gtatgcgggt tatgcttaat tatagaatat gcctaataaa aataaatcca taacacttaa
    17761 tcagaaaatt attattatcg attcctaacg attgaataca tcagctcctt taatttagat
    17821 ggcattatac gaaaaaatgt tctcaacata gcattactta ttaattcatt ttttcgaata
    17881 aaaccaattt tatattgata atacaatact ttatactcgt acaataaata tgacaatcca
    17941 cgtcgagcca taagattacg accagttcgc atttttaata aaatatctgg aagatttgca
    18001 aatcttgcat tatgtacaat taataggctc cacaatgcaa aatcttgaga ttttctgaat
    18061 ggaggataac caccaacagc taatactgta ttctttctaa aaattacaga aggatggcta
    18121 actgcgcttc gtttcctcgc gaatttaact atttctctat gttcgagagg cactttgcgt
    18181 gttgaaataa actcctcagt aacagtttca atttcatcaa taaaactgcc acatacatct
    18241 atttctgaat tattaatcat aaaagaaatt tgtttctcaa accgatgagg caaagaaata
    18301 tcatcagcat ccattcttgc cactaactca ttcctacaag cctttaatcc ttcatttaag
    18361 gcattagcca atccaacatt tctaggtaaa ggtacaaatg ttactatttt attgccaaca
    18421 tcatcaatga atgaatttat aatatcgatg tgtgtttgat ggagttctcc atctgcaaca
    18481 attactattt gatctggctt aagtgtttga tcgtgaaaaa tagagcgtag agccacctca
    18541 aaaaattgcg gtagatcatt tttataaatg ctaattaaaa ctgagaattt ttctaatcta
    18601 tgattcattt cattttacca cttcgaccca ttaaaccgtc attaatgcct tttaaaaaaa
    18661 aatataacct tttattacca tttggaagga aaataggata taaaaaaacc tttccaatta
    18721 atttaaccag actagaaatt ttccagtaga tgggtacata atttttattt aataaaagaa
    18781 agatatttcg agtagcataa taatgacgaa atgggcttgg caaaccgaca gaaagaatat
    18841 ttaagatctt aaatcgccca tctccaagtc tatgtgcaag taacgcattt ttattcctaa
    18901 ttactttaaa cccagcagct cttaatctcc aacaatattc atggtctacc gcatcgataa
    18961 aaagctcatc tttcattcct ccaacaatca accaactatt ttttggtatt agactgccag
    19021 aacttaatgt actatctacc tcataataaa cttctgtaag tggtttccct ttttttaccc
    19081 ttgctttatt taattcacca gttactttat caaaatcttg tgaaccaact aaaccaacat
    19141 tgacattttg tttaagcaat tttttgtaac aagtaagtaa ctgctctacc atcttaggat
    19201 caggaatact atcctgatcc atttgcaata taaaatcagc gccattttca aaagcccatt
    19261 tcattcctat actttgggct tctgctatgc ctaaattatc attgaaattg aatattttta
    19321 catcgcctga agaattttca gcatatttat aaccatttgt agagttattg caaacgacaa
    19381 ctttagtaac ttgtctcaac aataattcaa ccgcattttt taaatcatta tgttctgggt
    19441 tgtaagcaac caaaacggca tatacagtgt ccatcttcac cttaaaacct tcatttagct
    19501 ttcatctttt ttagaacatt acttaatgtc actaatacaa ttattacagc aacatggtta
    19561 gagtctaaaa tataaggatt agtaattgca taagaaacat atagaaaata tagcacacac
    19621 aactcactgt attttatgat tttaatcgtg agaaggagat taattaataa aaacaaagta
    19681 aataaaataa cgccaagttg atttaaaaaa taaactgact gcaattcata atatatatat
    19741 gcactataat cacggatagg agtttgaatt ttgatgacat tacccaaacc agaacctata
    19801 acaaaatttg atacagactc tgtaagatca ttaattaata cagtaaactg atcccatcta
    19861 actcctaaag aagaatcagc tccatttgat ttcatgatta tcaactcaat tgaatatgta
    19921 ataaaaaaag ggagaatcac agtaagaaaa accccaaaaa taattttcct taatttagcg
    19981 tatcgtgagt tagatttaga acatagtata atatacataa aaaacaagca tatcgaaaca
    20041 aaatatgcaa aattaccagc cactatagta cctatagcca gaataacggt tattgtattt
    20101 ttgaatcgat aatagaaata atcttttatg actatatgca acataaaggc aaatggaatg
    20161 agagcatttc ctttaatttg aactctatag aaaccacttc catatgtata aacatcacca
    20221 taatcattct ccaaaaaata atgtcttagt gctgaataat caccaatacc atatgttttt
    20281 gtcatataaa tactaatgat ggatataata accgcctgta ataccattaa atataaaaat
    20341 attttaacaa tcgagatggt tccataagag cagaaataag cacataatat aaataatatg
    20401 ataatataaa acctaattat tatcgctata tcgttaccct tgatatagga ataaataaaa
    20461 tttataaaaa gagctaatag aaatattaaa ataacaggat agtgatatat tccgtttgca
    20521 attttctttg taaatgacat gatacaaaga cataaaaacc cctccataat ccaactatat
    20581 tgaataaatg gaaagctacg tgtaaggaaa aatataaacc caaaaaacaa aagaacactt
    20641 aaacttttgt cttttgagtt ataaaaatca gaagtcatgt ttgcactcta attagatggg
    20701 cttgaggaag taatccctaa aatcaattcg ctattaatat ttcgtatcaa ttaataataa
    20761 tatcaaaaaa tctaacgatg ttcttacaga ccatgctatt gcggctccaa caattcccca
    20821 atgataaata aaaatatata atatgcataa atatgggata acttcgagca aatgaataat
    20881 agctgtaatt tttgatcttc cactagcctg aactgaaaca aatgggattt gtgcaatgca
    20941 attaaaaaag aaacctattg caagaatttt taatactata cctggcgtcc catgatatgt
    21001 aggtcccatc caagcggaca ttataaaatc tgataaaata attatcaaca ttacaattgg
    21061 aagtatacca ataaccatta taaaatatga taatatttta gtttgcttta ccgattgcaa
    21121 ttctgaactt aatcttggaa aaatagctct ggacaacgca cttggtaata tcgttaagcg
    21181 ttgtatacct tcagacggag cagtataaaa agaaacttta tcagccccca caatgtgtga
    21241 aagaataaaa cgatccatat atgtcataat agggctaata atattgctaa ctgttatcca
    21301 gcttccaaag ccgattaatc ttttaactgt tacaattttt acagacagcc cagatgatat
    21361 tattagtttt cgactaaata taaaggtcac tataagtgat aagactcttg ccataactaa
    21421 accatatata gcacttagta atcctccatg aaaaaaacag aaaatcactg gtaatccagc
    21481 cacaaaagag ttgttaattg attttattaa atttactttt ctgaactttt ccatcccctc
    21541 aaaaatcccc aaccagactt ggtttaacaa gtataagggt atggtagctg aaataatata
    21601 tattgctttg acagattcta caacatgatt cgcgttaatg tttaataatt taacaattac
    21661 attgctactc aaaaatagta cactaccgcc aatcaagccc aatatagtta gaattaccgt
    21721 tgaagttgaa atgatcgctc ttaattcttt atgaacattt ttatatattg atacttctct
    21781 tataacagct ctggtcaatc cagcatcaaa aatacttgca tatccaacta aggcaatagc
    21841 taacgtaaaa aggccaaatt gctcggtccc tagaattcta gacagtatac ctaacgcagg
    21901 aattgctatt aatgatggta taatataccc acttatattc cataaagtat tctttacaat
    21961 actcacaaaa ataattcctt catgttatgc aattctttag cccttgcatc tttaatcgat
    22021 aaaatataat tattatgttc tatcgtcggc cattttatgc tcagaatagg atcattccat
    22081 acaatccctc tatcactatc aggatgataa tagttcgtcg ttttatataa aaattccgca
    22141 gtctcgctca gcaccaaaaa accatgtgca aatccctcag ggatccacaa ttgccgctta
    22201 ttctcagcag ataaattcac cccaacccat ttaccaaagg taggcgacga tttacgaata
    22261 tcaacagcta catcaaaaac ctcaccaaca acgcaacgta ccagtttccc ttgcgcataa
    22321 ggttctaact gataatgcag cccgcgtaaa acacctttac tagacttcga atggttatcc
    22381 tgaacaaatt caaccttacg tcctacagct tcttcgaaaa ctttctgatt aaagctttcc
    22441 ataaagaaac cacgctcatc accaaaaact ttcggctcga aaattaacac atcaggaatt
    22501 tctgttttaa ttacgttcat tttattaata acctttaatc attttcagca gatactgtcc
    22561 ataagcattt tttttcagcg gctccgctaa tgctttcacc tgttcagcat caataaaccc
    22621 tttacggtaa gcaatttctt ctgggcagga aacctttagt ccctggcgct cttcaatggt
    22681 ggcaatgaag ttgcttgctt caataagact ctgatgtgtc cccgtatcca gccatgcata
    22741 accacgcccc atcatggcaa cggataaacg cccctgttcc atataaatac ggttaatatc
    22801 ggtaatttcc agttcaccac gggcagaagg cttaaggttt ttcgccattt cgacaacgtc
    22861 gttatcatag aaataaagcc cggttaccgc ataattactt tttggttgta gcggtttttc
    22921 ttccaggctt attgccgtac cgtttttatc aaactcaacg acgccgtagc gttcaggatc
    22981 attaacgtga taggcaaata ccgttgcacc actttctttg ttaacagcga catccattaa
    23041 cttcggcaga tcatgaccgt agaagatatt atcaccaaga accaaagcac aatcatcacc
    23101 accgataaac tcttcaccga taataaacgc ctgcgcaagc ccatctggag tcggttgcac
    23161 tttgtactga agatttagcc cccactggct accgtcacct agcagttgtt gaaaacgagg
    23221 agtatcctgt ggcgtactaa taatcagaat atcgcgaata cccgccaaca tcagtgtaga
    23281 gagcgggtaa tagatcatcg gcttatcata aataggtaat agctgtttac tgacagccat
    23341 agtcacagga taaagacgtg taccagaacc acccgctaaa ataatacctt tacgcgtttt
    23401 catttcatca ttccttttaa ttcatcttgc tccaccatca cgaacaagat gcaaaaacta
    23461 ttaaattgct gtagtcgtaa ttaattcgtt gagcattcgt ttcacaccaa cctgccagtc
    23521 aggcaagaca agcgcaaagt tctgctgaaa tttttctgta ttaaggcgag agttatgtgg
    23581 acgacgagct ggtgtaggat aggctgttgt tggtactgcg ttgagcttgt tgagtgcaag
    23641 gggaatacct gctttgcgcg cctcttcaaa aaccagcgca gcataatcgt gccaggttgt
    23701 ggtaccactg gctaccagat ggtacaaacc tgcgacttcc ggtttattca gtgccacacg
    23761 aatagcatgt gccgtacaat cagccagcag ctcagcacct gttggcgcac caaattgatc
    23821 atttatcaca gccagttctt cgcgctcttt tgccagacgc aacatcgttt tggcgaagtt
    23881 atttccttta gctgcgtata cccagctggt acggaaaata agatgcttcg cgcaatgttc
    23941 ctgtaacgct ttttctccgg ctaacttggt ttcaccgtaa acatttagcg gtgcggttgc
    24001 atccgtctcc agccatggcg tgtcgccatt tccagggaat acgtagtcag ttgagtaatg
    24061 aattacccaa gccccaactt cattagcctc ttttgcaatt gattcaacac tagtcgcatt
    24121 gagtaattgt gcaaattcgg gttctgactc agccttatct actgcggtgt gagccgcagc
    24181 attaacaata acatcaggtc gaattctttt gactgtttca gctacacctt caggattact
    24241 aaaatcacca caataatcag tggagtgaac atcaagagca atcaaattac ccaaaggtgc
    24301 cagagcacgc tgtagttccc aacctacctg ccctgttttg ccgaaaagga ggatattcat
    24361 tactggcggc cctcatagtt ctgttcaatc cacgattgat aagcaccact tttcacatta
    24421 tcaacccatt ttgtattgga caggtaccat tccaatgtct tccgaatccc gctctcaaac
    24481 gtttcctgcg gtttccagcc caattcgcgg ctaatcttct ctgcatcaat cgcataacgg
    24541 cgatcgtgtc ccgggcgatc ggcaacataa gtaatttgct cgcggtaaga tttctctttc
    24601 ggtacaatct catccagcaa atcacaaata gtgagcacta catcgatgtt tttcttttcg
    24661 ttgtgtccac caatgttata agtttcaccc gctttacctt cggttacgac ggtatataac
    24721 gcacgcgcat gatcttcaac atacagccag tcacgaattt gatccccttt gccataaata
    24781 ggtaatgcct taccttccag agcattcaga ataaccaatg gaatcaattt ttccgggaaa
    24841 tgataaggac cataattatt agagcaatta gtcacaatgg ttggtaaacc ataggtacgt
    24901 ttccacgcgc ggactaaatg atcgctggat gcttttgaag cggaataagg gctgcttggc
    24961 gcgtaagctg ttgtctctgt aaataagggt aattcttctg tattatttac ctcgtcagga
    25021 tgaggcaaat caccatagac ttcgtcagta gaaatatgat gaaaacggaa tctagttttc
    25081 ttgtcgctat caagagcaga ccaataattg cgagcggctt ccaaaaggac atatgtacca
    25141 acaatattgg tttcaataaa tgccgcagga cctgtaattg aacggtcaac atggctttca
    25201 gcagccaggt gcatcactgc atctggctga tgctgagcaa aaatccgtgc cattgcagct
    25261 gcatcgcaaa tatccgcatg ttcaaaaaca tagcgttcag aatcagaaac atcagcaagt
    25321 gattccaggt ttccggcgta cgttaattta tcgacattaa caacactatc ctgcgtatta
    25381 tttataatgt gacgaactac agcagaacca ataaatcctg cgccaccagt aacaagtatt
    25441 ttcacctaat ttattccata ttgcttcaga gcatgctgtg aaataagcgg ctctcagttt
    25501 gattaataga agtattaatg cacgctaccg cccctggctt tacagctacc agagcactgc
    25561 atgcatgcct acgatgtgac gagcgttacc cactcgcgct aaacccgaaa aattcaaaag
    25621 ctaattgtct taccaatccg ctctggaaac aaggaaaatc ctggaaaact ttgactaaaa
    25681 tcctattgct aactcgttgt tattctgatt gtttatataa aacaacggca ggaatattcg
    25741 caacaaatta ctttcaccac gaatcttcac tgccgttata attttcttat caaccgttac
    25801 atccggtcag attttcatta ttcgcttaac agcttctcaa tacctttacg gaacttcgcc
    25861 ccttctttca ggttgcgcag cccatacttc acaaacgcct gcatatagcc cattttttta
    25921 ccgcagtcgt agctgtcgcc ggtcatcagc attgcatcaa cggactgttt tttcgccagc
    25981 tcggcaatgg catcagtcag ctgaatacgt ccccatgcac caggctgagt acgttcaagt
    26041 tccggccaaa tatcggcaga aagcacatag cgaccaacgg ccatgatgtc tgagtccagc
    26101 gtctgcggct gatccggttt ttcgataaat tcaacaatgc ggctgacttt accttcgcga
    26161 tccagcggtt ctttggtctg gatgacggag tattcagaga ggtcacccgg catacgtttt
    26221 gccagcacct ggctacggcc cgtttcattg aagcgcgcaa tcatggcagc aaggttgtag
    26281 cgtagcgggt cggcgctggc gtcgtcgatc acaacgtctg gcagcaccac gacaaatgga
    26341 ttgtcaccaa tggcgggtcg tgcacacaaa atggagtgac ctaaacctaa aggttcgccc
    26401 tgacgcacgt tcataatagt cacgcccggc gggcagatag attgcacttc cgccagtagt
    26461 tgacgcttca cgcgctgctc aaggagagat tctaattcat aagaggtgtc gaagtggttt
    26521 tcgaccgcgt tcttggacgc atgagttacc aggaggattt ctttgatccc tgcagccaca
    26581 atctcgtcaa caatgtactg aatcattggc ttgtcgacga tcggtagcat ctctttgggt
    26641 atcgccttag tggcaggcaa catatgcatc ccaagacccg ctaccggtat aactgctttt
    26701 aaattcgtca ttattttcct acctctaagg ggctgatagt gcgtaaatta ttgtcatagg
    26761 ttagccaaac ggtatggcta tataccaagc ataactttga ttaaacctta cgataacact
    26821 acacaccatc agcatctggg ttactcggat tactcggaaa tccacatact gataatttaa
    26881 tcagtacctc tttccgaata atcgtagtcc aacctggtcc ttttttctct gactcgtctg
    26941 cattactcag aaacaaacgt tatgtcgtct tttttggcat ggacgaattc atactgcaga
    27001 gttcgatcca gaccttgcga cagcgtatac ggtgcaacaa aacctgaaga atgcactttc
    27061 gttgcgtcaa actgtgttgt tgcgcagaat tttttcacgc gcacagagct gacagcgtat
    27121 tttttgcccg taattttgct caggatatca aagcaatatc cacccagcat tcctagtggg
    27181 taaggcaagt gcatagaagg gatctttttg ttcaggcttt gttcaacttc agcaaccaac
    27241 tggttcatgt tcaggtctgg cttatcaaca tagttataaa cctcataacc tgcggcaaca
    27301 ttcttcagtt tgtacttgat aaactcaaca atgtttccaa cataagccat ggacttatag
    27361 ttagtccctg cgcccaccat cataaacttg ccgccagcga tctgtttcag caagttatag
    27421 acgttaccgc ggttgcgttc accgaagata acggtaggac ggatgatggt taatgaacgt
    27481 tctgttggtg ctttgttata ccattcacgc agcacttcct ctgcctgcca cttacttttg
    27541 ccgtagtggt tgaaagggtc gtgtggatgg ttttcgtcag ggttgtgttt gttcaaacca
    27601 taaacagcaa cggaactggt aaagatgata tttttaacgc catttttttc catggccgcc
    27661 agcacattgc gggtaccctg aacgttgaca tcataataga gagaagtagg gctgacgtca
    27721 tcgcggtgtt ccgctgccag tagtacaaca gtgtcaaaac cggctaacgc ctggtcgagt
    27781 gcctgttgat cacgaacatc accaatctgt gtgatttctg gataaaagtg gctctgccgt
    27841 ttgtccaggt tcttgatatt aaagtcagca attgccgttt caagtagtcg ggttcctacg
    27901 aatccggaag ctcctatgag caaaacgtta ttgttcataa atcactttag tctggttgtt
    27961 acgtaagaaa cacaagataa agatgagtac cttccctgag tagtcaatgc tgcccagccc
    28021 cagctttaac agttagtgtg aggattataa tcttttagaa cattatatcc agtaagttta
    28081 tgaatggtcg caaatctact ctctccgttc cggcaatcta aagttaatct tttccacatt
    28141 aacaatatgg tgattaatcc tgtcgatgtc gacggagctt tgccctcttt cattcatcgc
    28201 atgaacattt gcaagtgaca gcagtgtttc ttttttcgcc ataaagacac cgcgtacgtc
    28261 tttgcgcatg tcgaagttca tgctcaatgc tggtccaact gaggattcct gcatcacctt
    28321 gatattacgc ataaaaagat gttgcggttt gttgtgtaac tccagcgagg cacgcttcat
    28381 ctcaatgtta gtcagtgcca caaaggagac agcattcccg gcggagattt ggatgccgcg
    28441 caatttataa acaagatggg tgttatccag ttgaatatta ttcactcgga aattttgcgg
    28501 tatcgagaga tatttgcctt taattacccc atagccgatt aacatcccgg cgctattaat
    28561 catttcaata ttatcaatca cgaaattgtc acaaccgtaa atagcaactg tcgcgttatc
    28621 aatgcctgct ttcttactga aatccggcgt gatattgcgg gctttgatat tacggataac
    28681 aaaatgtttg ccattttcaa catggatcaa ctgccgacaa tccgatcccg tgatattcgc
    28741 cacgacaaag tttttcactg cctggtcttc cgggtagtta ttatcgtagg tactccccgc
    28801 aaggcctatg ccaattcccc agttgatttt gccattagta cagttgatgc gctcgatgac
    28861 atggtcggag atcaaaatat cactgtcgtt aattgccacg ttccattcaa tggcgtcgcc
    28921 ctgtaagtcg ctgaacttac aattggtgat gttggcaccg ataatctggt tatgaaatcc
    28981 ctggcgtaag atggcgtaat tagcgtggct gacagtgagg ttatcgatgg tcaggttgct
    29041 catgacccgt ttgtttttgc cgccgatata aatctgcgtt accgggccaa agccgctcat
    29101 cgccagccct ttgatggtgc agtcagaacc acgcacatcc aaggtgatgt tatgcatact
    29161 gccgccctcc tcccctgtca cctggctgcc gtcctgtaag acaaatcgcc ctctgccgtt
    29221 gccgcgcaag cttccaagaa tgtgtaacgt tttaccggga gggataaaga tgccggtgtt
    29281 gatattgtca caaaccaatc cggcaggcac gacgaccgta tgcccttcgc tgaaggcttg
    29341 tttaaatgag gcgatccagt cgtgtgggtt gtagtcgtta atgttaacgc tttgtcgggc
    29401 gggaagtgcg cgagcgaaag gggtatggag gaaggcaagc gccgagcttg ccgtcaggaa
    29461 ggtgcgtcgg gagagtgttt taaatggcat gcgttctcct ctataaagct tgcagcaagc
    29521 tggcgagttc tcgattgatc acctgctggt taaaatcgtg ttcgactttt tcgcgcgcac
    29581 gtttgacaac cggagccagt tcgtcggtgc ccagttggct aaacgccgcc agtcgttgcg
    29641 ccagtgcgcg agcatcgttc tcaggcacca gccagccgga tttgtcagcc tccaccagtt
    29701 ccggtattcc gctatgcaga gtagaaacca ccggaatacc gaccgccatc gcttccatta
    29761 gcgccaccgg aatgccttcc atatcaccat ccgcgcccgt taccgatggc aacagaaaga
    29821 catccgcgtc gtcgagcatc gctttcactt cgtggctcgg tttaaagccc ggcatctcta
    29881 ccacatcttc cagttgatat tgttcgatga gggtacgcag gcgtcgttcc cacgggccaa
    29941 tgccgaggat gcgatagcga aatgccacgc cctgctcttt caactgacgg caggcttcaa
    30001 tcgccacatg caggcctttt ttctcggtta agcgtgcgac ggaaatgatt tccagcggcg
    30061 ttgcgggcgc tttcaccgga cgcgggctaa agcgcgtcat gtccacgccc atacgcgata
    30121 cggcgatttt ttccctcggg cagcccattt tttgcagcct tccggcccac aaatcgctta
    30181 tcggtaacat caggtcgcca cggcgaaaca gttgttgata ttcgggagtg taatggttga
    30241 gcacttcccg actggaaata tcaatgccgt ggaaaatggt ggcaattttg ccgcgaatga
    30301 cacccagttc ccgtagtttt gctgcggtta cccccgccgg gccaaaatga gcgatgaaca
    30361 catcggcatg aagcggtgtt gcgacctggc cgcaaatggc agacaaaatc aggttccgcg
    30421 actcggcacc atagcgtttg aggttgagcg cctgccaggt atttttacga taaatgccgc
    30481 gtaacgtctg gctggcgcgg tggcgcagtt tcgctatttt gcccgtaggt tcgtcctgta
    30541 accagtgggt tctggcggca aggttgtatt tcgtccatgc cgcgtgggtg ttttgtgtgt
    30601 cgcctttttg cagcgcgaga atctctacct caaatcccat atcaataaac gcggtaattt
    30661 ggttaaggac gaaggtttct gacgacagcg ggaatttcag taaaaagaag ccgaccttca
    30721 tttcacctcc ccgatgcgct caagcacaga ctgcaccatc tgcattcctg tctgacgctc
    30781 acgactgacg gcttcactaa gtcgcgcatt cagtgccgga agctggccta aggtatccgc
    30841 aaccatcgct tgcaggctgc cgtctaataa atgacggata tcaattgcca tctccggtag
    30901 tcccagctgc tgcataatcc cggcggattt atgttcgtag ttgatggcaa ttgccggagt
    30961 ggcaaaattc atcgagataa tggcagagtg caggcgcgta ccgacggtga gttcacaggc
    31021 accgagaatt ttgcccattt ccagatcgtt gagttcatcc atcaccacat ggtaacgggc
    31081 aggatcgctg atgtgctggc gaagattgag cgccatcatg cggtcgtctt tgttatagct
    31141 gtcgataccc gtacaggtgg agagtgcaat gacctgatag ccttcgtcga gaatgcgatt
    31201 gaccaccccg gcaaaggctt tttcatacgc ttgttgagtg gtgccgaggc gtttgtcaaa
    31261 cggtgccagt tcgcgcaggg taattgctac cgttttctgt tgtgcggcaa cgtccagcca
    31321 gtgttgaaca gcatagctgg cggtgaagtc ttcggtgtgg tgatcgacca gccacgcggt
    31381 atcgacgcca tgttccactt ttgcggtagt gatattgctg cgtttcatca gatcaagact
    31441 gaccgattcg cgcaggatca gcgcgtcaca gtgaccaaaa acgtagttcg ccagttggtt
    31501 aaattgctca tcctggaagg ggccgacgct gtgaccaatc ataaacagcg gctttttcgc
    31561 cataaacgtg caaagcgcat gttcaaactg cggcacgccg tagagatcga caaaaaacga
    31621 tcctccgacc tgaataatgg cgtcgtaacc tgagagcagg cgcacgaagt cggtgaatcc
    31681 ctgggcgatg gcgatattac gcagcttgcc agtgtcggtg acgcgtgaga gcaatacctg
    31741 gtgttgatag cggcgacgga ggactttttt aacgcgcccg acaacgcccg ccgcgctgtt
    31801 gtgttgtttc atttgcagga acagaggatc gcccattacc gggcggttga gtagccagga
    31861 agaactgacc ggatagcggc tcatcacgtc cacttcggct tgtggattga ggatgttgat
    31921 ggcatcaagt aagccgcgca agatggcgct gtcgccacga ttgccgcaag tgtggttgcc
    31981 cagaataagt aatttcattt tttcctcata aatttgatgc caagtgaggc tgtgtttgtt
    32041 ttgttcgttg ccgctttttg cctgatgcga cgctgacgcg tcttatcagg cctacaagac
    32101 ccgagcacag aaccgtaggc cggataaggc gttcacgccg catccggcat tcagtgcctg
    32161 atgcgacgct gacgcgtctt atcaggccta caggtcccga gcacagaacc gtaggatgga
    32221 taaggcgttt tacgccgcat ccggcaaccg ttgtcggaac cgaaaacagc aactcacccc
    32281 gcccgtaaaa gcattttcat tttttcgctg cgacaaaact gacgcttcac ttccaccacc
    32341 agcggatggc gcgacagcac aatcatcacc acaaacgcca gcacgcccgc ggctatttgc
    32401 accgccagca gcatccccag cgccagttgc cctttcagca caatgcccag cgcataactg
    32461 accaccagcg tcggcaacga gagataaaac ggcagccaca aactcaggat gtactggcga
    32521 taactggaac caagaaccgg tttaatcatc acgaagtaac tcagaatggt gttgataatc
    32581 tgcaccagca ggaaaccgag cgtgacgccg attgcgcccg ccatctgccc accaataaca
    32641 atcgccggaa taaacagaaa tgttttgaat acgttgaatt taaagctgat atcgacccgc
    32701 gctttcgcca tcagcagcga accaatcggg ttacccacgg agcgcagtag ccccaccaca
    32761 cacagaaatt gcagcaccgg aataatgctg ttccacttct caccaaagac cagtggtaca
    32821 aagttattcg acaccaccat tagcccgagc agcgccggaa agttgataat ccccaccacc
    32881 gacagcggtt tgtagaagtt aatacgcagc ttttcggtgt cgtcctgaat tttggcaaac
    32941 gccggaaaca acacacgggt gatgattggg ttcagcttca tcggcggcac aacggccacg
    33001 ttgtaagcca ggttgtagcc ccctgccacg cccgcgccga gaatacgcgc cagcaccagc
    33061 gttgaaaggt tggtattgag atagttgatg atgctgtccg ccgtcagcca ggcaccaaag
    33121 cgtaagttcg gtgccaccga cgccagcgag aaatgcagac cggggcgata aattttgcgg
    33181 ccaaagtagc caaacagcag cgttctcacc gcactattga ccagataacc gaggatcgcg
    33241 gtcatcgcca gcggccagaa atgggcgcta accaccgtaa aagtgaagcc cgccagtacc
    33301 gcgctggttt cgatcatgcc gattttgttg aactccagct ccttttgcat caacgcgcgg
    33361 aactgctgcc cctgggggat caccacaaac gccagcgaca atgttttaat caacggtgcc
    33421 aggtccgggt tattcagcac gtcgccgatg agatcactca acaaaaacac cgccagacac
    33481 accacaagcc ccagcccgac gttcagccag tacagcgtgg tgagttcaag gtggctgatt
    33541 tcttttcgct gaataatcga gttagcgata ccgaagtcag aaagcgtatc tgccagcgcg
    33601 atgatcacca gcgacacagt aagcaaaccg aactggtggt tgtcgataat ccgcgccagc
    33661 acggtcatct gcaccagccc gaggccgatg atgatcaccg tggcaatcgc cgaccacttc
    33721 gcgccgctga tggttttttc acgtaagctc atatcaatat gccgctttgt taacgaaacc
    33781 tttgaacacc gtcaggaaaa cgattttgat atcgaaccag acgctccatt cacggatgta
    33841 ctcaaggtcg aactcgacgc gtttttccat tttctccagc gtgtcggttt cgccgcgcca
    33901 gccgttaatc tgcgcccagc cggtaatgca cggtttcact ttatggcgca gcatgtagcc
    33961 ttcaatgagc tggcgatact gttcgttatg cgctactgcg tgcggacgtg gaccgacaat
    34021 cgacatcccc ccggtcagca cattgataaa ctgcggtaat tcatccagcg aggtgcggcg
    34081 cagaaagttc cccactttag tgacgcgcgg atcgttctgc gtcgcctggg tcaccacttt
    34141 gtcgttctcc atcactttca tggaacggaa cttccacact ttgatcggct tgccatccat
    34201 gccgtagcga gtctggcgga aaataaccgg ccctggtgaa ctgagtttca ccgccagcgc
    34261 aatacagcac agcaccgggg agatcagcag caagatcagc gtcgccagca caatgtcttc
    34321 cgcacgtttt agcaggcggt taaccccgga aagcggcgtg tcatacagcg gcaccaccgg
    34381 aacgccgttc atctcttcga gacgtgaatg gagaatattg aaggtaaaga catcggggat
    34441 cagcagcacc gaacaggtgg tgtccgccag ttgatggacc agttttttca ctcgcgcgcc
    34501 gtcgcacatt tgcatcgcga tatagacgtt atgaatctta cccgctttag catcctcgac
    34561 cagctgttgc agattgcccg cccagtcgtt agaaacaccg cccggtttcg ggtcgtggta
    34621 aacgcccacc acttcaaacc ctaaccacgg ctgattacgg aagctctcca tcagcatttg
    34681 tccggcggct aaatcccccg ccaccgcgac catgcgcttg ttatagccat gattacgcag
    34741 ccagcccgcc ccaatgcgaa tacatgaacg gcaaaccacc agtccgatac tggtcagccc
    34801 ataccacgcc agccagattt tcagttgcgt gtcgaaatca ttgttgaacg ccaccagtcc
    34861 ggcgctgaaa atcacgctta aggtccagtt ttgtagcaac agggcaaatt ctgtcgctgc
    34921 ccgaacaccg cgccatgagc gataaaaatc ggtgatgccg cccagcatct ggaacaccac
    34981 cagcgtaatc aacgccacca acaggtgcat gtagaggaat gacagtccgc tcacttcgca
    35041 aaccagccat agtccggcaa acatgatggt gatatctgaa aagcgttgca ccatagagat
    35101 taacgatgca ttggttttcg ctcgcgcttt tttagatttg tcatcgttgt tcctgttatt
    35161 agccccttac ccgcagtggg gtaagggaag atccgacatt actcgttcag caacgtcagc
    35221 agagttcgcg ttcgcgcttc catcagcggc acatcaccgc gtgattccac attcaaccgc
    35281 accaccggtt cggtgttgga ggagcgcaga ttaaagcgcc agtcggcaaa ggtcatgctg
    35341 atgccatcgg tgcgatccac cgccagcgca tcacggctaa aatgctgttc cacgcggtta
    35401 atcgcctcaa cgggttgcgc cagtttgctg ttgatctcac cgcttgccgg aaacgccgcc
    35461 atccggtcgc gcaccagttc gcccagcgtt tttcctttca ggcacaccag ttcggcgacc
    35521 agcagccacg ggatcatgcc gctgtcgcag taagcgaaat cacggaagta atggtgggcg
    35581 ctcatttcgc caccgtagat ggcgtcttcc ttgcgcatac gttctttaat aaaggcgtgt
    35641 ccggttttcg acatcaccga cgtgccgcca gcggcagtca ccacatcaac ggtattccag
    35701 gagagacgtg gatcgtggat gatcttcgcg ccgggatttt tttcgaggaa cgcttctgcc
    35761 agcaggccga caatgtagta gccttcgata aactgccctt tttcgtcaaa caggaagcag
    35821 cggtcaaaat cgccatcaaa ggcaatgccc atatccgcgc cgtgtttgat gaccgcattg
    35881 cgggtgtcgt cgcggcattc cggcagtagc gggttaggaa taccgttggg gaaattgccg
    35941 tccggcgtgt tgtgcacttt gattaattcc acgggtgcgc caagggcttt aaagcgggct
    36001 tcaatggcgt ccaccaccgg acccgctgcg ccattcccgg agttaatcac cagcttgagc
    36061 ggcgtgaggt ttttgacatt gatataaccg aacaggtgat caacgtaagc gtcacgcagg
    36121 ttgatttgct gatagcgacc gcgtttggtt tcatcgacgg gaggaaagtc gttggcttca
    36181 gccagacgct ggacgtcgcg cagtccggta tcaccgctga tcgggcgagc cccttcgcgc
    36241 accagcttca tgccgttata atccatcgga ttatggctgg cggtgacttc aatgccgcca
    36301 tccacgccga gatggaacgt ggcgaaatag atctcttcgg tgccggacat accgatatcc
    36361 agcacgtcaa cgcccgcatc ctgtaaacct tttgccagcg ccagttttaa ggtttcgctg
    36421 gtgagacgga catcaccgcc taacacaatg gttttcggtt tgagaaattc gccataggcg
    36481 cgaccaatgc gccaggcgat atcttcattc agttcttcgc ctaatttccc gcgaatatca
    36541 taggctttaa agcaggttaa ttttttcatg tcgttaccct ttttcaggca atagttggcc
    36601 ctgaccgaaa cgaccaattt tgtttttgtc gttatttaca tctcggtttc gcattcattg
    36661 cctgatgcga cgctgccgcg tcttatcagg actacaaacc cgaaccgtag gtcggataag
    36721 gcgttcacgc cgcatccgac aaacagcgcc tgatgcgacg tttacacccg tccgtagcga
    36781 tccgcgaagc gcaccacatc atcctcttcg agataagagc cggagcgcac ttcaattaaa
    36841 tcaagcggaa ttttccccgg attttccagg cagtgcgtcg cccccagcgg aatataaatg
    36901 gactcgtttt caccaagcag tttgatatca ccatcaatgg tgacttttgc cgttcccgcg
    36961 accactaccc agtgttccgc gcggtgatgg tgcatctgta ccgacaaacc ctcgcccggt
    37021 ttcacggtga tgcgtttcac ctggtagcgg tcgcccgcgt cgatagagtc atatttgccc
    37081 cacggacgat acacttcgcg atgcacccga tgctcatggc gaccatcggc tttgatctgc
    37141 tcgaccactt ttttcacatc ctgtaccgcg ttacggtcgg caatcagcac cgcatctttg
    37201 gtctgcacca ctaccagatc tttcaccccg acggtggtga ccaggccaga ttcggcatac
    37261 acatagctgt tttcagtttt gtgattaatc acatcgccgt ggcaaacgtt gccctcggcg
    37321 gtgtgggcgc tgatctccca taatgaagac caggaaccaa catcgctcca gcccgcatcc
    37381 atcggcacca caacagcatc tgccgtacgt tccatgaccg cgtaatccac cgactcttcc
    37441 gggcaggcga gaaacgcttc ttcatccaca cgaataaaat caagatccgg atcgacggag
    37501 ctcatcgctt tttcacaggc atcgagaata tccgggcgat attttttcag ttcttcgaga
    37561 tagcgtccgg cgcggaacag gaacatgccg ctgttccagt aatattcgcc gcttgccaca
    37621 taggcctggg cggtttccag attcggtttt tcgacaaact gcgccacttc aaaggccacc
    37681 gtatcctgct cacccgcaga cacttcaccg cgacgaatat agccataacc ggtttctggt
    37741 agatccggca caatgccaaa ggtcaccagc ttgcccgctt cggcatatgg catggcatta
    37801 cgcacggcgg cacggaacgc gtcttcatcg gcaattacat gatccgccgc cagcaccagc
    37861 attaacgggt cgttctccgg gctatgacgt tttgccgcca gcgccgccag cgcaatggcc
    37921 ggtgcagtgt tacgccctgc aggttcgaga ataatgttct cggtgagttt gttcagttga
    37981 cgcagctgtt ccgcgacaat aaagcggtgc tgctcattgc aaatcaccac cgggctttcg
    38041 cactccacgc cgttcaggcg gcagatggtg gtttgcagca tggtgagatc gcctttcagg
    38101 cataaaaact gcttggggta aagtacgcgg gaaagcggcc ataagcggct accggagcca
    38161 cctgccatca caactggata gagtttcgac tgcgccataa ttatccccga atatcattta
    38221 taaattgacg taacacgttc tctttatcga gcgtgcgttc ggcatattca cgtgccaccg
    38281 tgttgtgttt gggcagcagg agcgcctgac taatccccgc caccagcgcc tcgaccgatt
    38341 ccggctctac gcaaaccgca atgcccggaa aggtttcgca aagctgcccc agttctgtgt
    38401 gggcttctgc ggtaatcacc gcgttaccgc ctaccgccag aatattggtc agtttcgacg
    38461 gcaatacggc atctgccgcg ccgcattttt gcaccaccag atggcaatcg cccatcttca
    38521 gcagtgcggg taaagcgtca tacgattgca gcgggaaaaa ttgcatgttg cgcagtccac
    38581 gctgctgcgc cattttttcc agccgcgctt tgccgccgcc ctgcccgaca atggcaaaaa
    38641 tcagcggttc atcgcgcagg cgatcggcag cttcaataac gttttccagt ccctgctttt
    38701 caccaatatt gccggagtaa agaatgattt ttttgttatc cggtaggcca agctggttac
    38761 gaagggcatc aacatcggcg tctgcaacat gctgaaaacg ggcgatttcc gaccagttgg
    38821 ggaagaaaat gatgttatcc gccgccacgc ctttttcgat ggctttattc atcatcgaac
    38881 gcgaaatcgt ggagacgtta tcgacgttat gcagtccgct acgttcgaac gccgttgcca
    38941 gctgtgccac tttgccgcct ttgccttttc cggcaaggcc cagccccagc atggcgtcca
    39001 cttcgtaatc ctgaatatgc agcacggtac gcgcaccaga gagtttcgcc agcaggcgca
    39061 ttcccggcgt gcaaaacagc gttggcacca cgccgataat gcgatccggc ttccagcgac
    39121 gttgcgccat caacgggaaa aaactgctga cggcaaaacc gccgagatgc aacaggcgtt
    39181 tcagggtgct cggctgtttt ggcacgtaca gcgggcagcg ccacaccgtg gcggccccct
    39241 cttctcgttt gtagcgctag gcggaatagt tctcgcccac ctgccactgc gggtagtaag
    39301 gcggtgcggt aataacccgc acctcatgac cttgcgccgc cagccattcc accatttctc
    39361 cggtgtattt gccgatgccg gttaactccg gcgagtagtt aatgccgtag accagtattt
    39421 tcataatccg ggtactccgg cacgcttctc agcgagaaaa taggcgcggc tgttagcgtg
    39481 aacattgtca ctggcgagca gtgcgtccgg cgtcagccag cggtaatcgt catgctgctc
    39541 atccggcagt aacagttctt cttccgctac tctgaagcga aaaccgagca ccacatagtg
    39601 agtggtgaaa tccgtgccag agaagttatc gtcataaaag tgctgccaga caccgtaaaa
    39661 ctggcctgct gttatcggca gacgcagccc cagttccgcc atcgtcagcc gctcaaatgc
    39721 ggcttccagc gtttcgtctt tctgcacgcg ccctcccggc acaaaccagt aaccctgcgc
    39781 cgggcggttg gttcttttgc caggcagaaa ctcgccgcga ctgttctcga caataaagtc
    39841 gagagagaca agcggtgtgg agcgcactac cgtggcaaag tcttcctgac gtaaaaacat
    39901 cattaccccc gaaagcggtc ttgattctca aggaaccact ggtaagtgct ggcaagcccc
    39961 gcttccagtg agatttcgtg ataccagcca agctgatgca ggcgcgtcac atcaagcagt
    40021 ttgcgcggcg taccatccgg tttactggca tcaaaaacca cccgaccttt gtaacccacc
    40081 actttggcga tggtttgcgc cagctcgcgg atagtgcagt caacgcccgt gccgacgtta
    40141 atgtgcgaca gcatcggctg ggtgttttcc agccagactt cgtgcgccag ctccatcaca
    40201 tgaatgctcg ccgccgccat atcatcgacg tgcaggaatt cacgcatcgg tgtaccgctg
    40261 ccccacacca ccacgtccgg cgcattctgt gccgtcgcct cgtggaagcg acgcagcaat
    40321 gctgggatca catgcgaatt actcgggtgg aagttgtcat gcgggccata caggttggtc
    40381 ggcatgaccg agcggtaatc tcgtccgtac tggcgattgt aagattcgca cagtttgatc
    40441 ccggcgattt tggcaatggc gtaaggctcg ttggtcggct ccagcgtgct ctgcaataac
    40501 tcgctttctg ccatcggctg ttttgccagt ttcgggtaga tgcaggacga tccgagaaac
    40561 agcagtttgt tcacgtcgtt ctgatgcgcg gcgtgaatga tgttgctctc aatcatcatg
    40621 ttctggtaga tgaaatccgc cggataggtg ttgttggcaa caatgccgcc cactttcgcc
    40681 gccgccagat agacctggtc aatgcgttcg ctggcaaaga aatcatgcac ggcgcggctg
    40741 tccagcaggt tcagctcgtc gcgggtacgt aataccagct ccacatcacc gcgctgttcg
    40801 agctgccgct tgatggcaga accgaccatc ccacgatggc cagcgataaa aatgcgttgt
    40861 ttactcatgc ttatgactcc agcgcgatcg ccacgtcgta gccgtgagat ttcagcagag
    40921 agtgtttttt cgccgcttcg aggtcattag ccaccatttc agacaccatc tctctgaggg
    40981 tgatttccgg tttccagccc agtttttcgt gcgctttggt cgggtcgtcg agcagcgttt
    41041 caacttctgc cggacggaag taacgcggat caacggcgat aatcacatca cccggtttaa
    41101 cgcccggcgc gtcatgcccg gtgacggaaa ccacaatgcc cttctcttca acacccgtgc
    41161 cttcaaagcg cagtttgatg cccagctgtg ccgctgccat ttccacgaac tgacgtacgg
    41221 agtactgaac gccggtcacg ataacgaaat cttccggctg ttcctgttgc agcatcatcc
    41281 actgcatttt tacgtagtct ttggcgtggc cccagtcacg cagggaatcc atattgccga
    41341 ggtacaggca cgactccagc ccctgggcga tgttggcgat tgcgcgggtg attttgcggg
    41401 taacgaaggt ttcgccgcgg cgcggggatt catggttgaa gagaataccg ttacaagcgt
    41461 acatgccgta ggattcgcag tagttaacgg tgatccagta ggcgtacagt ttggcgaccg
    41521 catacggaga tcgcgggtag aacggcgtgg tttctttctg cggaatttcc tgcaccagac
    41581 catacagttc agaggtggaa gcctgataga aacgagtttt cttttccaga ccgaggaagc
    41641 ggatcgcctc cagcaggcgc agcgtaccca tcgcgtcgac gtcagcggta tattctggtg
    41701 actcaaaaga gaccgcaacg tggctcattg cgcccaggtt gtacacttca tccggctgta
    41761 cttcacgcaa aatgcgcgtc aggttagagg tatcactcag gtcgccataa tgcagatgga
    41821 atttcgggtt gcaggtgtgc ggatcctgat aaatgtgatc cacgcgctcg gtgttgaatg
    41881 acgatgcgcg acgcttaata ccatgcacct cgtaaccttt ttccagcaga aactctgcca
    41941 ggtaagaacc gtcttgtccg gttacaccgg tgatgagagc gacttttgac atgtcttatt
    42001 cctctgtatt tttgaattta ttcagtttca acgcgttcgc gtatcaccac tgcgggattc
    42061 ccccggcaaa ccacatttac cggaagcgat ttaaaaacac tgcttcgtgc acccacgacg
    42121 gtgccgtcgc cgattgtgac gcctggggca acaaagacat cggttgccag ccagcatttc
    42181 tcgccaatca caataggcg
//
LOCUS       DAJTOR010000010        41642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTOR010000010 DAJTOR010000000
VERSION     DAJTOR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.010"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..255
                     /locus_tag="OO249_000526"
     CDS             <1..255
                     /locus_tag="OO249_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5634934.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO249_000527"
     CDS             complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO249_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5634935.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO249_000528"
     CDS             complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO249_000528"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5634936.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2907..3182)
                     /locus_tag="OO249_000529"
     CDS             complement(2907..3182)
                     /locus_tag="OO249_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5634937.1"
                     /translation="MSRTIFCIFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO249_000530"
     CDS             complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO249_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5634938.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4423..5142
                     /gene="trmB"
                     /locus_tag="OO249_000531"
     CDS             4423..5142
                     /gene="trmB"
                     /locus_tag="OO249_000531"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5634939.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLNNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5142..5468
                     /locus_tag="OO249_000532"
     CDS             5142..5468
                     /locus_tag="OO249_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5634940.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5652..6371
                     /locus_tag="OO249_000533"
     CDS             5652..6371
                     /locus_tag="OO249_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5634941.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6547..7593
                     /gene="ansB"
                     /locus_tag="OO249_000534"
     CDS             6547..7593
                     /gene="ansB"
                     /locus_tag="OO249_000534"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5634942.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7710..8717
                     /locus_tag="OO249_000535"
     CDS             7710..8717
                     /locus_tag="OO249_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5634943.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQSAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OO249_000536"
     CDS             complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OO249_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5634944.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(10001..10594)
                     /locus_tag="OO249_000537"
     CDS             complement(10001..10594)
                     /locus_tag="OO249_000537"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5634945.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVTQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(10602..10892)
                     /locus_tag="OO249_000538"
     CDS             complement(10602..10892)
                     /locus_tag="OO249_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5634946.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OO249_000539"
     CDS             complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OO249_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5634947.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGMWMAL"
     gene            complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OO249_000540"
     CDS             complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OO249_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5634948.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            12195..13175
                     /locus_tag="OO249_000541"
     CDS             12195..13175
                     /locus_tag="OO249_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5634949.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OO249_000542"
     CDS             complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OO249_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5634950.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(13767..14330)
                     /locus_tag="OO249_000543"
     CDS             complement(13767..14330)
                     /locus_tag="OO249_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5634951.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTASADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OO249_000544"
     CDS             complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OO249_000544"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5634952.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OO249_000545"
     CDS             complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OO249_000545"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5634953.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OO249_000546"
     CDS             complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OO249_000546"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5634954.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(17015..17512)
                     /locus_tag="OO249_000547"
     CDS             complement(17015..17512)
                     /locus_tag="OO249_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5634955.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OO249_000548"
     CDS             complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OO249_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5634956.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OO249_000549"
     CDS             complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OO249_000549"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5634957.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(20877..21092)
                     /locus_tag="OO249_000550"
     CDS             complement(20877..21092)
                     /locus_tag="OO249_000550"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5634958.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            21228..21359
                     /gene="yqgB"
                     /locus_tag="OO249_000551"
     CDS             21228..21359
                     /gene="yqgB"
                     /locus_tag="OO249_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5634959.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            21368..23344
                     /gene="speA"
                     /locus_tag="OO249_000552"
     CDS             21368..23344
                     /gene="speA"
                     /locus_tag="OO249_000552"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5634960.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            23481..24401
                     /gene="speB"
                     /locus_tag="OO249_000553"
     CDS             23481..24401
                     /gene="speB"
                     /locus_tag="OO249_000553"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5634961.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OO249_000554"
                     /gene_synonym="yggG"
     CDS             complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OO249_000554"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5634962.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            25643..27634
                     /gene="tkt"
                     /locus_tag="OO249_000555"
     CDS             25643..27634
                     /gene="tkt"
                     /locus_tag="OO249_000555"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5634963.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            27948..28391
                     /gene="cmtB"
                     /locus_tag="OO249_000556"
     CDS             27948..28391
                     /gene="cmtB"
                     /locus_tag="OO249_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR5634964.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISHG"
     gene            28419..29807
                     /gene="cmtA"
                     /locus_tag="OO249_000557"
     CDS             28419..29807
                     /gene="cmtA"
                     /locus_tag="OO249_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR5634965.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSLGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDTGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            29822..31099
                     /locus_tag="OO249_000558"
     CDS             29822..31099
                     /locus_tag="OO249_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5634966.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            31096..32061
                     /gene="yggF"
                     /locus_tag="OO249_000559"
     CDS             31096..32061
                     /gene="yggF"
                     /locus_tag="OO249_000559"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5634967.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            32083..32592
                     /gene="fumE"
                     /locus_tag="OO249_000560"
     CDS             32083..32592
                     /gene="fumE"
                     /locus_tag="OO249_000560"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5634968.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            32589..33302
                     /locus_tag="OO249_000561"
     CDS             32589..33302
                     /locus_tag="OO249_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5634969.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(33274..33951)
                     /locus_tag="OO249_000562"
     CDS             complement(33274..33951)
                     /locus_tag="OO249_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5634970.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(33945..34622)
                     /locus_tag="OO249_000563"
     CDS             complement(33945..34622)
                     /locus_tag="OO249_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5634971.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(34610..35317)
                     /locus_tag="OO249_000564"
     CDS             complement(34610..35317)
                     /locus_tag="OO249_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5634972.1"
                     /translation="MHPFTSLILWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(35318..35896)
                     /locus_tag="OO249_000565"
     CDS             complement(35318..35896)
                     /locus_tag="OO249_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5634973.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(35919..36350)
                     /locus_tag="OO249_000566"
     CDS             complement(35919..36350)
                     /locus_tag="OO249_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5634974.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            36722..37741
                     /gene="epd"
                     /locus_tag="OO249_000567"
     CDS             36722..37741
                     /gene="epd"
                     /locus_tag="OO249_000567"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5634975.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            37791..38954
                     /gene="pgk"
                     /locus_tag="OO249_000568"
     CDS             37791..38954
                     /gene="pgk"
                     /locus_tag="OO249_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5634976.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            39169..40248
                     /gene="fbaA"
                     /locus_tag="OO249_000569"
     CDS             39169..40248
                     /gene="fbaA"
                     /locus_tag="OO249_000569"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5634977.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            40606..41466
                     /gene="mscS"
                     /locus_tag="OO249_000570"
     CDS             40606..41466
                     /gene="mscS"
                     /locus_tag="OO249_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5634978.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
BASE COUNT        10192 a        10411 c        11091 g         9948 t
ORIGIN      
        1 attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa
       61 ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg
      121 gcggttcaac gttatttcct tgcactggaa gaaggggtaa atttgttgcc gggattttcg
      181 ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt
      241 tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc
      301 gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca
      361 tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac catacggtct
      421 gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc acgatgccgc
      481 cgaggataca gccgaagccg ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa
      541 ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg
      601 cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga gtcgggtcgc
      661 cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc attacgttct
      721 taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt tcagagatct
      781 gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca aacatcggat
      841 ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc agttccgcgc
      901 ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac agcgcgaatg
      961 catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca accggaatat
     1021 gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca atatacagct
     1081 gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg ataaagccga
     1141 tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg gcattttgca
     1201 ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca aacgagttaa
     1261 tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg aacagcgtga
     1321 tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat ttgtcggcca
     1381 caatccccag cagcgcaggc ataaagaccg ctgcgatacc cagtgagcta taaactgcgc
     1441 caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg gtcgtcagcc
     1501 aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc ttaagattca
     1561 tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt tgaccgtgcg
     1621 agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg caaataattg
     1681 caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg cccctgagca
     1741 tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg gtattcactt
     1801 tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc tgataaacat
     1861 cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc gaaaagactc
     1921 gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc cgcgatgttg
     1981 ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg gtgccggtat
     2041 caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg
     2101 agcggaacac atctttcccg gcagtatgtt gtaccacctg cattaatccc agcgcatcgg
     2161 aacggctgac cgcatacggg ttaaaggaag attcggtctg cataattgcc agaatcagcg
     2221 actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac
     2281 gtttatcaag gtggttcggt accatgttaa tggtgacgct gtagatgata cgcagcccgt
     2341 tactgcggct ttgcagacgg tttttcagca gataatccgc gaagttgctt gcgcgacctt
     2401 cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg
     2461 aaatcgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg tcacccatca
     2521 gcaacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg gcgatagttt
     2581 cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt
     2641 atttcacgta gtccttagga ccggcgatca ccacctcttt gaatccccag atgttctcaa
     2701 tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg acccaggctt
     2761 cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag
     2821 ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt tatgcaagtt
     2881 gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata gccctcgata
     2941 tgcacctctt taccctcgaa caggaagttg accatctcct gctcaagcag cttgcggtgc
     3001 tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgctt gtgctgccac
     3061 tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc gccggggtac
     3121 agctgaaaat cctgaccttc tgcttcacgt tgcaggaaaa tacaaaaaat cgttctgctc
     3181 ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg taactgctgt
     3241 aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa gttataccag
     3301 agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca cataggcaca
     3361 atatctaagt ggaaatggct gaaggtatgc cgaaacgcgg tcagttgcgt caggttatcg
     3421 gcagcaatct gccgttgcgc cagccactgc cgcaaacttt cttcgtcggc aaactgcggg
     3481 aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa tacttcatct
     3541 tcgtgctgta acagcaaaaa gtagccggtg cgctccggca gcgtctgttt cggttttttg
     3601 cccggataaa gcgaccagct attattggcg gcggcaatac atccgttttg tagcggacag
     3661 agcgaacatt tcggcttcga gcgcgtacaa atcatcgcgc ccaaatccat catcgcctga
     3721 ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca taatttattc
     3781 tcgacctctt ttttcccagg ccagccgctt acagcatagc agcgcgccag cacgcgtttg
     3841 acgttaccgt cgagaatcgg aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg
     3901 gtggaacgcc cgacgcccgg taacgccgcg acttcttcaa aggtttccgg gaatttaccg
     3961 ccgtgtaagg tcgccacttg ttgtgccgct ttatgcagat tgcgcgcgcg ggcgtaatag
     4021 ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg cattggcgag atcggtcacc
     4081 gtcgggaagc gcgccataaa gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt
     4141 tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg ccagggcagc
     4201 gtttttcgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc
     4261 atggtcaccg aattcactgt tgttgggggc aagattgcag cacagcgacg gcagggtgta
     4321 aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact aactttggat
     4381 aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt
     4441 tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc
     4501 caggggcgac tgaccaaagg ccaggaacat gcgctggaaa actactggcc ggtgatgggc
     4561 gttgagttca gcgaagatat gctggatttc cccgcgcttt ttggccgtga agcgccggtg
     4621 acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa agatcgccct
     4681 gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct
     4741 gcgcatgaag aaggtttaaa caacctgcga gtgatgtgtc acgatgcggt tgaagtgctg
     4801 cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc tgacccgtgg
     4861 cacaaagcgc gccataataa acgccgtatc gtacaggtgc cgtttgccga actggtaaaa
     4921 agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc ttatgcggaa
     4981 catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga gagcaatgat
     5041 tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg tcatcgtctt
     5101 ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag aaccgtagcc
     5161 gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt tcggtggcat
     5221 ggcgattccc ggaaggtaca tcggaagaac agattgataa aaccgttgat gattttatta
     5281 acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg gcctgggaag
     5341 gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg attgtgcgta
     5401 agtggctgga agagcgcaaa ctggatgagg tacgcaccag cgaacttttc gacgtttggt
     5461 gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat
     5521 caggcctgga aagatgcacg atcgagtagg cgggataagg tgtttacgcc gcatccggca
     5581 tggaaaacgc gtactttgtt atcaatctgg ggccagcaaa tgctggcctg attttttctt
     5641 gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg acggcaatga
     5701 ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc
     5761 aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac ggcaacgtga
     5821 tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg aaggattatc
     5881 aggctgaact acgcagcacg ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaaa
     5941 aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgaaagc agcaaaatgc
     6001 gcagccgtct gaccaaactt gatgcgcaac tgaaagagca gatgaaccgc attatcgaaa
     6061 cgcgcagcga tggcctgacg tttcactata aagccattga tcaggttcgc gccgaaggcc
     6121 agcaattagt gaatcaggca atgggtggaa ttttacagga cagcattaat gaaatgggcg
     6181 cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga agcctggggg
     6241 ggctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc cagcagtttg
     6301 gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc ctggtcggga
     6361 atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt ttaattcttc
     6421 gttttgttac ctgcctctaa ctttgtagag ctccaaaata tattcacgtt gtaaattgtt
     6481 taacgtcaaa tttcccatac agagctaagg gataatgcgt agcgttcacg taactggagg
     6541 aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg ttttagtggt
     6601 gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat tgccggtggt
     6661 ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga aaatctggtt
     6721 aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt agtgaatatc
     6781 ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat taacaccgac
     6841 tgcgataaaa ccgacggctt cgtcattacc cacggtaccg acacgatgga agaaaccgct
     6901 tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg cgcaatgcgc
     6961 ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt ataacgcggt agtgaccgca
     7021 gctgataaag catccgctaa tcgtggcgtg ctggtggtga tgaacgacac cgtactggac
     7081 ggtcgcgatg taaccaaaac caacaccacc gacgtagcga ccttcaagtc tgttaactac
     7141 ggtcctctgg gatacattca caacggtaag attgactacc aacgtacccc ggcacgtaag
     7201 cacaccagcg atacgccatt cgatgtctct aagctgaatg agctgccgaa agtcggcatc
     7261 gtttataact acgctaacgc atccgatctt ccggctaaag cactggtaga tgcgggctat
     7321 gatggcatcg ttagcgctgg tgtgggtaat ggtaacctgt ataaatccgt gttcgacacc
     7381 ctggcaaccg ccgcgaaaaa cggcactgca gtagtgcgtt cttcccgcgt accgacgggt
     7441 gctaccactc aggatgctga agtggatgat gcgaaatacg gcttcgtcgc ctctggcacg
     7501 ctgaacccgc aaaaagcgcg cgtcctgctg cagctggctc tgacgcaaac caaagatccg
     7561 cagcagatcc agcagatctt caatcagtac taatcacctc gctccggtat cgtgccgggg
     7621 ctttttcact tcagacttac gtccattgcc aattttcatt accctaatga taatcaccgg
     7681 aataaattat ttcgcgtgag gtttttcggg tgaaaaagca atggattgtt ggcacggcac
     7741 tgcttatgtt gatgactggt aatgcctggg cagatggcga accgccaact gaaaatatct
     7801 taaaagatca attcaaaaag cagtatcacg gcattctcaa gcttgatgct atcaccttaa
     7861 aaaatcttga tgctaagggt aatcaggcca cctggtcagc ggaaggcgat gtctcttcca
     7921 gtgacgatct ctatacctgg gtcggtcagt tggcagatta cgaactgctc gaacagacct
     7981 ggacgaaaga taaaccggta aaattctcgg cgatgttaac cagtaaagga acgcccgcgt
     8041 ctggctggtc ggtgaacttt tactcttttc agtcggcagc cagcgatcgt gggcgggtgg
     8101 tcgacgatat caaaacgaat aataaatatc tgatcgtgaa tagcgaagat ttcaattatc
     8161 gctttagtca gcttgaatct gcgttgaata cccagaagaa ctcgattcct gcgctggaaa
     8221 aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg gatgcatatt
     8281 ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga agcctttaaa aaaattcacc
     8341 aacagcgtga tgagtttaat aaacagaacg atagcgaggc gttcgcggtt aaatatgaca
     8401 aagaggttta tcaaccggcg attgcggcat gtcataaaca gagtgaagag tgttatgaag
     8461 tgccgattca gcagaaacga gatttcgata tcaacgaaca acggcgacag acttttctgc
     8521 aatcacaaaa actcagccgt aaattgcagg atgactgggt aacgctcgaa aaagggcaat
     8581 atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt ttgatgaaaa
     8641 ttgacgatat caaccaagct aatgaacgct ggaaaaaaga taccgaacaa ctgcggcgca
     8701 atggggtgat taagtaagtt tgatatagtg cacgatttgt agcaggatgc ggcgttgacc
     8761 ccgcatccgg caatcaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgt
     8821 tgccacatgt aggctggata aggcgttcac gccgcatccg gcaatacaag tttactcagc
     8881 cagaaaaagc tccagcagcg aatttaaaaa cagcttccca tgttccgtta tctgccagta
     8941 atccgcacat tctgtgagat aaccctgggc aatcgcctcg tctaactgtg ggcgaatcac
     9001 atcttcgcaa agcccggtat acgcactaaa ctctgcgcgt ggcgcggcct ccagcaagcg
     9061 aaagcggttc ataaagaact caaacggctt atctgcggct tcgacatcac gctggctttc
     9121 cagatacctt ccttgcataa aaccacgagg atgacgcgtt ttagtggtac gcagaatgcg
     9181 cccatccggg aaggtgactt tgccgtgcgc gccgcagcca ataccgatgt agtcaccaaa
     9241 tcgccagtag ttgagattgt gctggcactg ataaccgggt ttggcgtaag cggaagtttc
     9301 atattgctga taacccgctg cggttaataa ctgatgcccc tgttcgaaaa tatcccacag
     9361 cgcgtcatcg tccggcagca ccggtggtcg cgaaccaaac agcgtattgg gttcgatggt
     9421 cagttgatac caggaaagat gcggcggatt cagttcaatg gcctggcgta gatcgccaag
     9481 cgcctcttcc agtgattgat ccggtagtcc atgcatcaaa tcaaggttaa agctacgtaa
     9541 ccctaaaccg ctcgccagct ttgccgctcg tttcgcttct tgcgggccat gaatgcgccc
     9601 aagtcgtttc agcttttctt cgctaaaact ctgcacacca atagagatgc ggttcacacc
     9661 agcacgctga taatcgacaa agcgatcggc ttctaccgta ccagggttcg cttccatagt
     9721 aatttctgca tccgctgcca gcggcaaacg cgcacgcacg ccgtccagca gcgtttgcat
     9781 cgccgggccg gaaagcaggc tcggcgtacc accgccaata aagattgtct ttacttcacg
     9841 gccctgagcg taagccacat cattgtccag atcgcaaagc agatgctgaa cataatcgtc
     9901 gtgcggcact tctcctttca acgcgtgaga gttgaaatcg cagtacgggc atttctgcac
     9961 gcaccacggg atgtgaatgt agagactcag cggcggtaat ttaaccatta cgtaaagcgt
    10021 ccagcagcag tttcaacgcc tgaccacggt gggaaatggc gctcttttct tcgcgggtca
    10081 gttcggcggc ggttttccct tcggaaggta cgaagaagat tggatcataa ccaaagccac
    10141 cagtgcccgc tggttcgcga gtaatcacgc ccggccagct gccgtggcat accagcggag
    10201 tcggatcttc cgcgtgacgc agatatacca gcacgcagtg gaaacgtgcc tgacgttggt
    10261 cgtccggtac gtctttcagt gtttccaaca gtttttgcag attcttttga tcggtcgcgt
    10321 cttcaccgga ataacgcgcg gagtaaatac ccggcgcgcc acccagcgca tctaccgcca
    10381 gaccagagtc gtcggcaatt gccggtaaac cggtcacttt tgccgcatgg cgcgctttca
    10441 gaatcgcgtt ttcgataaag gttaggccgg tttcttcagc ggaatcaacg ccgaggtctg
    10501 tttgtgtcac gatatcaaga ccaaagtcgc taagcagcga cgccagctca cgcactttac
    10561 cggcattgcc ggttgcgagg acaacttttt gcataggata cctaattaat taacgccgcg
    10621 atttctggcg ggatttgttg cggattaatg attttaattt gtttgtggcg gccaagttcg
    10681 cctttttcaa tcaccacctg gcttttggca acccggaatt gcttaccgag aaacttcacc
    10741 agatgactgt tggcctggcc gtcaactggc ggcgcggtaa tggcgacttt aacttcgtcg
    10801 ccatgtaaac cgacaataga atcacggctg gctttcggct gaatatagag ccgtaaaacc
    10861 agaccgtcat cattaactgt tacggcattc ataacgccat ccacatcccc ggcagcagga
    10921 tgtttccggt tgcctgtaat acttctgcga cacccatatt gatgacatac agcagcagaa
    10981 cgaggatcat cggcgagaaa tcaatcccac ccattgccgg tagcaagcgg cgaatcgggc
    11041 gcagcagcgg atcggccagc tgaatcagca cgtattcaat cgggctacga ccctggctta
    11101 cccagctcat aatcgccatc accagcagga cccagaaaat cagcaagccg atggttttca
    11161 gcagaatcag taaaccggca atccagatga ttggcaggaa ggtcaccact ttaaacagca
    11221 caatggcttt gataaaactg agaatatagg caaccagcag cgaggcgctg tcaatgggac
    11281 ccattgccgg aataacgcgg cgcagtggcc cgataattgg ctgcgttacc ttcactacaa
    11341 actgtgagaa ggggttgtaa aaatcacaat gagcccactg catccagatg cgtaataaca
    11401 gcaccatggt atacagctca atgaccgttg aaagcaggaa agtcaacgta ttcatggcgt
    11461 tcctttaatt ccttattttt tagagtaatc acgcgcacca aaaattgcag tgccgatacg
    11521 aaccatcgtg ctacctgccg caatggcggc ttccatatcg tccgacattc ccagagagag
    11581 cgtgtcgata tgcgggtagc gcgttttcag tccggcaaat gctacagcca tttggcgtgc
    11641 aacttcaaac tgccttacat attctgactc aggcgcaggg attgccatca gcccgcgcag
    11701 acgtaaacgc ggtagttcag cgaccgccgc tgccagctcg tccagttctg ccagttgaat
    11761 cccggactta ctgttttcat cactaatatt aatttgaatc agtacgttaa gagggggaag
    11821 ttctgccggg cgctgatcgt tgaggcgggt agcaatgcgc aaacggtcga tggtatgaca
    11881 ccagtcgaaa tgctctgcca ccaggcggct tttattagac tgcaacgggc caataaaatg
    11941 ccattctaat cctgttacgc ccagttcctg aaagtggcga attttatcta ccccttcctg
    12001 aacgtagttt tcaccaaatt gacgctgccc ggcatcaatg gcttctgcga tggcgctcgc
    12061 aggttttgtt ttactgactg caagcagcgt aatttcttct ggagaacggc cgcaacgcgt
    12121 tgcagccgct gagattttgt cccggacctg tgccaggtta tgcgcaatat cgttcatttt
    12181 ccgaggatct tagtatgaat atggaagaaa ttgtggccct tagtgtaaag cataacgtct
    12241 cggatctaca cctgtgcagc gcctggcccg cacgatggcg cattcgcggg ctaatggaag
    12301 ctgcgccgtt tgatgcgctg gacgtcgaag agctactgcg ggagtggctg gatgacgatc
    12361 agcgggcaat attgctggaa aatggccagc tggattttgc cgtgtcgctg gcggaaaacc
    12421 agcgattgcg tggcagcgca ttcgcgcaac ggcatggcat ttcgctggcg ttacggctgt
    12481 taccttcgca ctgcccgcag ctcgaacagc ttggcgcacc aacggtattg ccggaattac
    12541 tcaagagcga gaatggcctg attctggtga cgggggcgac ggggagcggc aaatctacca
    12601 cgctggcggc gatggttggc tatctcaatc aacatgccga tgcgcatatt ctgacgctgg
    12661 aagatcctgt tgaatatatc tatgccagcc agcgatgttt gatccagcag cgggaaattg
    12721 gtttgcactg tatgacgttc gcatcgggat tgcgggccgc attgcgggaa gatcctgatg
    12781 tgattttgct cggagagctg cgtgacagcg agacaatccg tctggcactg acggcggcag
    12841 aaaccgggca tttggtgcta gcaacattac atacgcgtgg tgccgcgcag gcagttgagc
    12901 gactggtgga ttcatttccg gcgcaggaaa aagatcccgt acgtaatcaa ctggcaggta
    12961 gtttacgggc cgtgttgtca caaaagctgg aagtggataa acaggaagga cgcgtggcgc
    13021 tgtttgaatt attgattaac actcccgcgg tggggaattt gattcgcgaa gggaaaaccc
    13081 accagttacc gcatgttatt caaaccgggc agcaggtggg gatgataacg tttcagcaga
    13141 gttatcagca gcgggtgggg gaagggcgtt tgtgacgttg ccatgcccga tgcgcaagtt
    13201 tatagggcct ataaaggcaa taaatgtctg atgcgacgct aacgcgtctt atcagaccta
    13261 cagccgctgc cagatgtgtg ctggataagg cgttcacgcc gcatccggca agatggcggt
    13321 aacgactatc cgccaggctt ttagatcgcc ttaatatccc tgctcgaaat agctttcgag
    13381 aataataacc gcagaggcag agtcgacttt gcctttgttg agcgcccgat agccgccctg
    13441 ttcaaacaga ccggaacggg cttccacggt gctaagacgt tcgtcatgga gctttacttc
    13501 aacaccgaaa cgaccatgaa tacggttggc aaatttacgc gctctggcag tcaatggttg
    13561 ctcggtgccg tccatattca gcggcaaacc gacgatgatt tcgtccggct gccactcttt
    13621 cagtaaacgt tcgatgaggt tccagtccgg cgtaccgtcc tgtgctttaa ttgcaggcaa
    13681 agggcgagca gtgccggtaa tgcgttggcc gaccgctacg ccaatgcttt tggtgccgaa
    13741 gtcgaaggcg agtaaggttc cactcatcag gcgtgccctg ccacaccagg catggtgaga
    13801 atatccacac caatcagttt tgccgcctcg cgccagcggt cggcaatcgg cgttttgaac
    13861 agaatattca gatccgccga ggccgttagc cacgcgttat cgagaatttc ttgttccagt
    13921 tgaccttttt cccaggaggc atagcccaga gccaccaata cgtcagacgg ttgtttatcg
    13981 gtgccgagcg tttccagcac atcgcgggaa gtggtcatca ccgtgttgtc tgaaatgcga
    14041 atgctggaag caaaattgga gggcggagta tgcaaaataa acccgcgatc ttcagccagt
    14101 gggccgccga gcataacagg tttatccaga cggattgatt catcacgcgg ctccggcgtg
    14161 atcttcagct tttccagaat cccttcaatt ttgagatttt ccagcggctt gttgacgatg
    14221 atccccattg caccattggt attatgttcg caaatgtaga ccacggaacg acggaaaatc
    14281 ggatcctgga gagcaggcat ggcaataaga aagtgatgct gtaaattcat tgtcagaggt
    14341 tctgtttcct ggttcaaaaa agcaacagcg cccagtatgc gtggaaagaa gggcgctgtc
    14401 actcagagtc tcaacgagat ccttctcgct aaggtgggtt actgctgctg taaacgtgct
    14461 tcgatggcat ccattaacat tccggtgatc gacaccggaa actctgcttc aatctcacga
    14521 atacaggttg ggctggtgac gttaatttca gtcagacggt cgccgatgat atccagacca
    14581 acaaaaatca gccctttttc tttcagcgtc ggcccgatct gacgggcgat tttccagtca
    14641 ctttccgtca gcggccgagg ttcaccgcga ccaccggcag ccagattgcc acgggtttcg
    14701 cccccctgcg gaatacgcgc caggcagtac ggtaccggct cgccatccac caccagcacg
    14761 cgtttgtcgc catctttaat ggctggcagg taattttgcg ccatgcagta gcgagtgcca
    14821 tgctcagtca gggtttcggc aatcacgccg aggtttggat cgccttcttt cacgcggaaa
    14881 atcgacgcgc cgcccatacc gtccagcggc ttaagaatga tgtcgctgtg tttctcccag
    14941 aacgctttta gctgcgcttt attgcgcgta accagcgttt ctggcgttaa gtcagagaac
    15001 caggcggtaa acagtttctc gttacagtcg cgcaggctct gcggcttgtt aacgatcagc
    15061 gtccctttct cttcggcacg ttccagaata taggtcgcgt agataaactc ggtatcaaac
    15121 ggcgggtctt tacgcatcag gagcacatcg agatcggcca gcggcagatc ctgttcaccg
    15181 acgaacgaaa accactcttc gtagttctgc ttcacgttca gcgtgcgggt atgggcgcga
    15241 gcttcgccat tgatcagata cagatcgccc atctccatat agtgaagttc gtaaccacga
    15301 cgctgtgctt ccagcaacat agcgaaactg gaatctttct tgatgttgat gtttgcgatg
    15361 gggtccatca cgatgccgag cttgatcatt attcttctcc gttagcccaa atcgccaaat
    15421 cgtacttgta gcgcggtaat ggcggtgagc gcagttgtct ctgtacgcaa aacgcgaggt
    15481 cccaacagga tatcagtaaa ttgatagcgg gcagtcatgg caatttcatc tgccgataaa
    15541 ccgccttccg ggccaatcag caggcggacg cgttcaaccg gtaacggcaa cgtattgatg
    15601 ctgttactgg cgcgcgggtg aagattcagt ttcagtcctt catcctgctc tgcacaccag
    15661 gcttccagat ccatcgctgg acggatttcc ggcacccggt tacgaccaca ctgctcacag
    15721 gcagcaattg caatcttctg ccactgctgg agcttcttgt tcagacgttc actatccagt
    15781 ttaacgccgc agcgctcaga aaaaagtggc gtaatgaggc ttacaccgag ttcgatcgat
    15841 ttctggatcg taaattccat tttttcacca cgcgacatca cctgaccgag gtgaatatgc
    15901 agcggagatt cgcgatcgtc gatcttgcct tccagcacct tcacttccac gctttttttg
    15961 ctggcgctgg taatttcggc gtcaaagacc tggttgctac cgtcaaacaa ttgcaacgcc
    16021 tgccccggcc ccatgcgcag tacgcgcccg atatggttgg cggcatcttc gcaaagcgcg
    16081 atgtgagaat ggctggtcag tggttcagga tgataaatgc gggggatacg catagttaaa
    16141 aatccgcgtc gtctccccac gcggttgtac gcgtggggta ggggttaaca aaaagagtcc
    16201 cgctagtgta ggttagctct ttcgcgcctg gcaagcgcgt tgcacatacg ggttatgatt
    16261 gccttgcacc ttcgcgatgc gttcatcgcg ctcgcactcc cagtcggtaa ccggatacat
    16321 cttgttccat gcgttgaaca gctgcgtttg ctggcgagag agtgtcaggt tgtattggtc
    16381 gcgcatatag aagtaggtgc gcgcaatggc accgcgtgca cgcgctggtg gttcggcagc
    16441 tttttctttg aaatcgacct tcatggcgca ttgaccgtac tggccttcgc cgccattcca
    16501 ctggctgtac ataaagttgc cgcgatcgcc attcacctca ccgactgacg gctgcaggtt
    16561 atgcatatcg ctttccatct tgcgatagac cggatcttta gcgcagtttt tacgtccacc
    16621 gtcctgccag cactggcgct ggtgaccgaa ctgccaggcg ggaacgacgt gttcccactc
    16681 tacgcggctg gctcggtttt catttttgcg cacctgatag ccgcacgatt gcagatcaac
    16741 aacgcctttt ttgccctgcc agttaatttt acatccgcaa taaaacgtac cgggcgcgtc
    16801 agcgtggact tttaccgccg cggctttcgc ctgagaaaaa ctattgatac cttcggccaa
    16861 cgccgggccg gaaaatgctg cgctcagtac caccgcagca atagacaaat aacggtacat
    16921 cacttactcc gtgttaaaac gagccagcaa cgtagcgaaa gttgttttgg tatgcaatca
    16981 gccagatcag gaaagttcct gatagttcag atggttattt tgcgaccagt tgttcgccgc
    17041 agtgaacaca gcgatagacg gcttcgccac gaactacgcg attatggcgg cgtacggtaa
    17101 gctgatgctc ctggcacttg cagcggtagg ggaaggtgtt gcgacgcacg gattgcagtt
    17161 cgaactgatg cgtacgacgg gcgggaacac ccagcacgtt ttccatcatc cacttccact
    17221 ctttgccatg tggtgctacg cgcccgaaat gtttccatac cagcaaatgt gccagttcgt
    17281 gcggcaccac ttcttcaata aaggcttcac tgttttccaa cagcaaaacg ggattgaggc
    17341 ggatttcata gctttccagc caggctgttc cggcggaggt gccgcgctgg gtataagaga
    17401 gttttggctc cgggtagtta cgccccaatt tcaggttagc ctgggcgagt ttttcccgca
    17461 ggcgacgcat aacggcctgt tggatggcga tagggagacg ggatgttttc atagcggcag
    17521 aggatagagc gaagaaaaga tgactgcaag aggtggcttc ctccgcgatg ggaggaagct
    17581 tggggagatt aatcgtgagc gcctatttcg cgcagtttac gacctttcat cagattacgt
    17641 tcaatatgtt ccagcgaaac gtgtttggtt tccggtacca gccacaatgt cagcaggata
    17701 aacagtacgt tcagagccgc atacacccag aaggtgttgg cgttacccag tgtgttgagc
    17761 atggtcagga acgttgcgcc aacgatcatg ttggcaatcc agttggtggc ggtagagcag
    17821 gtgatgccaa aatcgcggcc tttcagcggc tgaatttcgg agcacagtac ccaaatcagc
    17881 ggaccggcac tcatggcaaa accgacaata aacatcagca gcatggcgat ggcgaaatac
    17941 tgcgctgacg gagagtgaat accgatatgc atcattgtac cgagtacgcc catgccagca
    18001 gccatcacca ggaagcccag cgttagcgtt ggtttacgtc cccagcggtc aacaaggccg
    18061 attgcgataa aggtggcaag tacgttggtc aggccgacaa tcactgtccc ccacatttgc
    18121 tcggtagtgt tggtataacc cgccagttcg aagattttcg gcgcgtaata catgatgacg
    18181 ttcatcccgg tgaattgctg cattacctgt aacagtacgc caaggaacac cgcgcggcgg
    18241 aagttgctat tctctttaaa cagcgcccag ccactctgtt taacctgcaa actttcacgg
    18301 atttcatcca gttcgcgttt cgcttccgcg ctggtgtcac gcaggcgtag cagcacgcgt
    18361 tcggcatcaa caaaacggcg tttggcggca aaccaacgtg ggctgtctgg caggaagaag
    18421 acaccaatca gcagcaaaat tgccgggatg ataatcacac ccagcatcca gcgccatgca
    18481 ccggtgtagc tgaaggcggt atcagaaaga taagcaccga ggatcccgat agtgatcatc
    18541 aactgataca tcgagatcat actgccacga attttttccg gcgcaatttc agagaggtac
    18601 agcggtgcgg tataagaggc cacacccacc gccagcccca gtagaacgcg ggaaagaatc
    18661 agtacttcaa cgtttggcgc agccgcagag aacagcgaac cggcaacaaa caaaattgcg
    18721 ccgatcatca ggctcttttt gcgcccgagt ttaaaggaga gccagccgct gcccaccgca
    18781 ccgactgccg caccgaacat catggagctt acgacccatt cttgcgtgtg cgaagtaatc
    18841 tggaattcat ctgcaataaa cggcagtgcg ccagcaatta caccgatatc caggccaaag
    18901 agtaatcccg ccagagcggc aaggaagcag acgaaaaacg tcattgcctt gtttgaccgc
    18961 ccctgttttt tagcgtcagg catgatgccc tccaatatgg ttatttttta ttgtgaatta
    19021 agataggtga gtacgacgta aaaagatgtg aagcaaatca catcagtgta atcgcttaca
    19081 ctaacattta tcttagttat ggtttaagtg catgaaaata attgagttat atcatcatag
    19141 attaaacgct gttatctgca attaagactt tactgaaaag aaatgtaaca actgtgaaaa
    19201 ctgaataata atatgaaaat attacaaata tttggctggt gttattggtg taatcggttt
    19261 cagtagtgat gataatttgc gataaaacgt gttgaggaag aatgatgtta ttgtgcgggc
    19321 agctttttgc cggatgcggc gtgaatgcct atccaccgcg gtgaggtaaa agaaaaaggc
    19381 cagcgcgagg ctggcctttg aaagcaggtg aagaaagatt acttcagacc ggcagcatcg
    19441 cgcagcagct gcgctttgtc ggttttttcc cacgggaaat gttcacgacc aaagtgaccg
    19501 tatgctgcgg tttctttgta gatcgggtgc agcagatcca gcatctgaat cagaccgtat
    19561 gggcgcaggt cgaagaactc acgtaccagc agagtcagtt gttcagaagg cactttctca
    19621 gtaccgaaag tttctaccat gatggaggtc ggttcagcca cgccgattgc gtaggaaacc
    19681 tgaatttcac aacgatcggc caggccagca gcaacgatgt ttttcgcgac ataacgtgct
    19741 gcgtaggctg cggaacggtc cacttttgat ggatctttac cagagaatgc tccgccaccg
    19801 tgacgtgcca tgccgccgta ggtatcaacg ataattttac gaccagtcag accgcagtca
    19861 cccatcgggc caccgataac aaaacgaccg gtcgggttga tgaagaatgt ggtggcagaa
    19921 gtcagccatt cagcgggcag aattggcttg atgatctctt ccattaccgc ttcttgcagc
    19981 gatttctggt cgatctcttc agagtgctga gtggaaagca cgacagcatc aataccaacg
    20041 attttgccgt cgtcatactg gaaagtcacc tggcttttcg cgtccgggcg cagccacggc
    20101 agagtgccgt ttttacgcac ttcagcctga cgctgtacca gacggtgtgc ataggtgata
    20161 ggtgctggca tcagcacgtc ggtttcatta gttgcgtagc caaacatcag accctggtca
    20221 cccgcgccct gttccagcgg atcggcacgg tcaacgccct ggttgatgtc aggagactgt
    20281 ttgccgatag cgctcagaac cgcacaggag ttagcgtcaa agcccatgtc ggaatgcaca
    20341 tagccaattt cgcgaacggt gttacgggtg atctcttcga tgtctaccca ggcgctggtg
    20401 gtgatttcgc cgccaactaa aaccatgccg gtttttacgt aggtttcgca agcaacgcgt
    20461 gctttcggat cctgttcgag gatcgcgtct aaaacggcat cagagatttg gtcagcaatt
    20521 ttgtcaggat gcccttcaga gacggactcg gacgtaaaaa ggtgttttgc catatttaat
    20581 atcacctaaa gagaatttgg ttagctcaaa ctgttgtgtg gattttctgt ggtagcggat
    20641 cctaccacga ctctgcaggt taaaaacact ggcagtctga gtgttaatcg gtatggatgg
    20701 attaacatct ggatggctat tttaggtcaa ttcttcaccc gatttccact tttttttgaa
    20761 tcgtgtctca ttctgttaaa aacgtggctg gaaatttttt ctgacaatgc cggcattctg
    20821 cgtatttata ttttgcaatt ttctgccatt gtggggtata aaacgcggcg cgcggcttaa
    20881 ataaaaagca cacgacgttt ctttcgtgtt gccacttcca gccgggttga aatcagagtt
    20941 ttggcttgtg ggttcgtctt aacaggcggc cgtggaggtg atacgaaata atgaaccgta
    21001 gtctgctgct taacctgtct caccgttctg gtgaagattc gttccccgca ctctgcatct
    21061 ctgctttgca tacctgccga tgttataccc atctcggcgc ttctcaggat tcaagagctg
    21121 gttacagtta ctgaggactg aacaagggcg ctcttgtaaa aacaagagtt ttctcgtggt
    21181 ttcgccgaac tgtcacactt acgttcggtt atgtgcttaa taatgttatg aaaaagaaac
    21241 cggttgcgca gttggagcgt cagcattcac tgctggaaaa tccatgtgct tatgggttgt
    21301 tatcgcagtt ccaggctgcg atagtcgtta actgttttac acttaataaa ataatttgag
    21361 gttcgctatg tctgacgaca tgtctatggg tttgccttcg tcagcgggcg aacacggtgt
    21421 actacgctcc atgcaggagg ttgcaatgag ctcccaggaa gccagcaaga tgctgcgtac
    21481 ttacaatatt gcctggtggg gcaataacta ctatgacgtt aacgagctgg gccacatcag
    21541 cgtgtgcccg gacccggacg tcccggaagc tcgcgtcgat ctcgcgcagt tagtgaaaac
    21601 tcgtgaagca cagggtcagc gtctgcctgc actgttctgt ttcccacaga tcctgcagca
    21661 ccgtttgcgt tccattaacg ccgcgttcaa acgtgcgagg gaatcctacg gctataacgg
    21721 cgattacttc cttgtttatc cgatcaaagt taaccagcac cgccgcgtga ttgagtccct
    21781 gattcattcg ggcgaaccgc tgggtctgga agccggttcc aaagccgagt tgatggcagt
    21841 gctggcacat gctggcatga cccgtagcgt catcgtctgc aacggttata aagaccgcga
    21901 atatatccgc ctggcattaa ttggcgagaa gatggggcac aaggtctatc tggtcattga
    21961 gaagatgtca gaaatcgcca ttgtgctgga tgaagcagaa cgtctgaatg tcgttcctcg
    22021 tctgggcgtg cgtgcacgtc tggcttcgca gggctcgggt aaatggcagt cttccggcgg
    22081 ggaaaaatcg aagttcggcc tggctgcgac tcaggtactg caactggttg aaacgctgcg
    22141 tgaagctggg cgtctcgaca gcctgcaact actgcacttc cacctcggtt cgcagatggc
    22201 gaatattcgc gatatcgcca cgggcgttcg tgaatccgcg cgtttctatg tggaactgca
    22261 caagctgggc gtcaatattc agtgcttcga cgtcggcggc ggtctgggcg tggactatga
    22321 aggcacccgt tcgcagtctg actgctccgt aaactacggc ctcaatgaat atgccaacaa
    22381 catcatctgg gcgattggtg atgcgtgtga agaaaacggt ctgccgcatc cgacggtaat
    22441 caccgaatcg ggtcgtgcgg tgactgcgca tcacaccgtg ctggtgtcta atatcatcgg
    22501 cgtggaacgt aacgaataca cggtgccgac cgcgcctgca gaagatgcgc cgcgcgcgct
    22561 gcaaagcatg tgggaaacct ggcaggagat gcacgaaccg ggaactcgcc gttctctgcg
    22621 tgaatggtta cacgacagtc agatggatct gcacgacatt catatcggct actcttccgg
    22681 cacctttagc ctgcaagaac gtgcatgggc agaacaactc tatctgagca tgtgccatga
    22741 agtgcagaag caactggatc cgcaaaaccg tgcgcatcgt ccgataatcg acgaactgca
    22801 ggaacgtatg gcggacaaaa tgtacgtcaa tttctcgctg ttccagtcaa tgccggacgc
    22861 atgggggatc gaccagttgt tcccggttct gccgctggaa gggctggatc aagtgccgga
    22921 acgtcgcgct gtgctgctgg atattacctg tgactctgac ggtgctatcg accactatat
    22981 tgatggtgat ggtattgcca cgacaatgcc aatgccggag tacgatccag agaatccgcc
    23041 gatgctcggt ttctttatgg tcggcgcata tcaggagatc ctcggcaaca tgcacaacct
    23101 gttcggtgat accgaagcgg ttgacgtgtt cgtcttccct gacggtagtg tagaagtgga
    23161 actgtctgac gaaggcgata ccgttgcgga tatgctgcaa tatgtgcagc tcgatccgaa
    23221 aacgctgtta actcagttcc gcgatcaagt gaagaaaacc gatcttgatg ctgaactgca
    23281 acaacagttc cttgaagagt tcgaggcagg tttgtacggt tatacttatc tggaagatga
    23341 gtaagtccgt attacttgaa tccgcttaat ttagcggtga taatccgcca caatttattg
    23401 tgacaaatcc aacccttcct cgtcgggcct aacgacgcgg aagggttttt ttatatcgac
    23461 tttgtaatag gagtccatcc atgagcacct taggtcatca atacgataac tcactggttt
    23521 ccaacgcctt tggtttttta cgcctgccga tgaacttcca gccgtatgac agcgatgctg
    23581 actgggtgat tactggtgtg ccgttcgata tggccacttc tggtcgtgcg gggggacgcc
    23641 acggtccggc agcgatccgt caggtttcga cgaatcttgc ctgggaacac aatcgcttcc
    23701 cgtggaattt cgacatgcgt gagcgcctga acgtcgtgga ctgcggcgat ctggtatatg
    23761 cctttggcga tgcccgtgag atgagcgaaa aactgcaggc acacgccgag aagctgctgg
    23821 ctgccggtaa gcgtatgctc tctttcggtg gtgaccactt tgttacgctg ccgctgctgc
    23881 gtgctcatgc gaagcatttc ggcaaaatgg cgctggtaca ctttgacgcc cacaccgata
    23941 cctacgcgaa cggttgtgaa tttgaccacg gcaccatgtt ctataccgcg ccgaaagaag
    24001 gtctgatcga cccgaatcat tccgtgcaga ttggtattcg taccgagttt gataaagaca
    24061 acggttttac cgtgctggac gcctgccagg tgaacgatcg cagcgtggat gacgttatcg
    24121 cccaagtgaa acagattgtg ggtgatatgc cggtttacct gacctttgat atcgactgcc
    24181 tagatcctgc tttcgcgccg ggaaccggta cgccagtgat tggcggcctg acctccgatc
    24241 gcgccattaa actggtacgc ggcctgaaag atctcaacat tgttgggatg gacgtagtgg
    24301 aagtggctcc ggcatacgat cagtcggaaa tcaccgctct ggctgcggcg acgctggcgc
    24361 tggagatgct gtatattcag gcggcgaaaa agggcgagta agcaccagat gcgatgcgca
    24421 cgggtaaaac gtgccattaa tgtcggatgc ggcgtgaacg ccttatccga cctacgttcg
    24481 gcacccgtag gccggataag atgcgccagc atcgcatccg gcaatgcgca caaggtaaca
    24541 aatgtgccat taatgtcaga tgcggcgtga acgccttatc tgacctacgt tcgacaccac
    24601 caggctttac ttaatcccat ccgcgctcat gcgatcgcga atatgctggg cgcgttcggc
    24661 ggatgccgga tggtcgtcaa acattgagct ttggcggcct tcttccagtt tcgccagttt
    24721 ttcaaagctg gtggcaagac ctgccgggct gatgccgcgt tggcgcagaa gatcgtaaga
    24781 ataatcatcg gcttctgctt cctggcgctg ggagaattgc gaattgacta atttctcgcc
    24841 cagatcacca agttgtgatt gagataaact gccgacaatc ccgcccgcag aggccgcagc
    24901 tactcgcacg gcatttgtac caagcgccac ctgcatccct ttcttcacat ggcctaacgc
    24961 cacgtgcccc atctcgtgac cgatcaccgc ttcgacttcg ttatccgtca tcatatccat
    25021 cagcccgcta tagacgcgga tacagccgtt agccattgca aaggcgttca catccttcgc
    25081 catatacact ttgtaattca ccggctgacc gttgatattg ttgcctagcg cattggcaat
    25141 agttgtcaga cgtttagcgt attcgctatt ggctggcgca atcgtcgcct tgctgtccat
    25201 ctcctgacat gcctgatcgc tcagggtttt cacctgcgca tcactcaaac tgtaagcctg
    25261 aaaagcttcc gctcctgatg agagcagtcc gttggagtcc atattctggc aaccagtcag
    25321 taccgttgcc acgctcattg ctaccaataa ggcgcgaatt ttcatgtttt ctcttccgca
    25381 caaggtcagg attattctaa aaatgcgccg tttgcaggtg aatcgacgct cagtctcagt
    25441 ataaggaaaa gcgcagcgga cgggcgagta gattgcgcaa catgcgagca tgatccagag
    25501 atttctgaag cggcaaaagg atgttccatg tacatgacgc gcggcttgcg gtaaattgtt
    25561 ggcaaatttt ccggcgtagc ccaaaacgcg ctgtcgtcaa gtcgttaagg gcgtgccctt
    25621 catcatccga tctggagtca aaatgtcctc acgtaaagag cttgccaatg ctattcgtgc
    25681 gctgagcatg gacgcagtac agaaagccaa atccggtcac ccgggtgccc ctatgggtat
    25741 ggctgacatt gccgaagtcc tgtggcgtga tttcctgaaa cacaacccgc agaatccgtc
    25801 ctgggctgac cgtgaccgct tcgtgctgtc caacggccac ggctccatgc tgatctacag
    25861 cctgctgcac ctcaccggtt acgatctgcc gatggaagaa ctgaaaaact tccgtcagct
    25921 gcactctaaa actccgggcc acccggaagt gggttacacc gctggtgtgg aaaccaccac
    25981 cggtccgctg ggtcagggta ttgccaacgc agtcggtatg gcgattgcag aaaaaacgct
    26041 ggcggcgcag tttaaccgtc cgggccacga cattgtcgac cactacacct acgccttcat
    26101 gggcgacggc tgcatgatgg aaggcatctc ccacgaagtt tgctctctgg cgggtacgct
    26161 gaagctgggt aaactgattg cgttctacga tgacaacggt atctctattg atggtcacgt
    26221 tgaaggctgg ttcaccgacg acaccgcaat gcgtttcgaa gcttacggct ggcacgttat
    26281 tcgcgacatc gacggtcatg acgcggcatc catcaaacgc gcagtagaag aagcgcgcgc
    26341 agtgactgac aaaccgtccc tgctgatgtg caaaaccatc atcggtttcg gttccccgaa
    26401 caaagccggt actcacgact cccacggtgc gccgctgggc gacgctgaaa ttgccctgac
    26461 ccgcgaacag ctgggctgga aatatgcacc gttcgaaatc ccgtctgaaa tctatgctca
    26521 gtgggatgcg aaagaagcag gccaggcgaa agaatccgca tggaacgaga aattcgctgc
    26581 ttacgcgaaa gcttatccgc aggaagccgc tgaatttacc cgccgtatga aaggcgaaat
    26641 gccgtctgac ttcgacgcga aagcaaaaga gttcattgct aaactgcagg ctaacccggc
    26701 gaaaatcgcc agccgtaaag cgtctcagaa tgctatcgaa gctttcggtc cgctgttgcc
    26761 ggaattcctc ggcggttctg ctgacctggc accgtctaac ctgaccctgt ggtctggttc
    26821 taaagcaatc aacgaagatg ctgcgggtaa ctacatccac tacggtgttc gcgagttcgg
    26881 tatgaccgcg attgctaacg gtatctccct gcacggtggt ttcctgccgt acacctccac
    26941 cttcctgatg ttcgtggaat acgcacgtaa cgccgtacgt atggctgcgc tgatgaaaca
    27001 gcgtcaggtg atggtttaca cccacgactc catcggtctg ggcgaagatg gcccgactca
    27061 ccagccggta gagcaggtcg cttctctgcg cgtaaccccg aacatgtcta catggcgtcc
    27121 gtgtgaccag gttgaatccg cggtcgcgtg gaaatacggt gttgagcgtc aggacggccc
    27181 gaccgcactg atcctctccc gtcagaacct ggcgcagcag gaacgaactg aagagcaact
    27241 ggcaaacatc gcgcgcggtg gttatgtgct gaaagactgc gccggtcagc cggaactgat
    27301 tttcatcgct accggttcag aagttgaact ggctgttgct gcctacgaaa aactgactgc
    27361 cgaaggcgtg aaagcgcgcg tggtgtccat gccgtctacc gacgcatttg acaagcagga
    27421 tgctgcttac cgtgaatccg tactgccgaa agcggttact gcacgcgttg cggtagaagc
    27481 gggtattgct gactactggt acaagtatgt tggcctgaat ggcgctatca tcggtatgac
    27541 caccttcggt gagtcagctc cggcagagtt gctattcgaa gagttcggct tcaccgtcga
    27601 caacgtcgtt gcgaaagcaa aagaactgct gtaattagca tttcgggtaa aaaggtcgct
    27661 tcggcgacct tttttattac cttgatatgt ccgtttgcgg acaagcaata gataaagcgt
    27721 gttgcagatc acaaatattt atatgcaata aatatcaatt atgtaatatg catcacgata
    27781 tgcgtattga catttgttgt tatatctata actcaatgtt atataagaaa ttaataattc
    27841 actgttttca aaacaccggt ttccctgctc aattgctttc attaaaccgc acgagtattt
    27901 tatttttacc gccggacggt accctaaaaa taaaataagg ataatttatg cggcttagtg
    27961 actattttcc agaatcatca atctcagtta tacattcagc aaaagattgg caggaagcta
    28021 tcgatttctc gatggtatca ttgctggata aaaactatat cagcgagaat tacattcagg
    28081 caattaaaga ttccaccatt aacaatggcc cttattatat tctcgcacca ggcgtggcaa
    28141 tgcctcatgc gcgaccggaa tgtggggcgc ttaaaaccgg gatgtctttg acattacttg
    28201 aacaaggtgt ttattttccg gggaatgacg aaccaattaa attactcatc ggactctctg
    28261 ctgccgatgc cgattcgcac attggcgcta ttcaggcgtt aagtgaatta ctgtgcgaag
    28321 aagaaatact cgaacaactc ttaacagcat catcagaaaa acaattagcg gacattatca
    28381 gccacggata attactctct cctctacagg ataatattat ggaaaacaag tctgctcgtg
    28441 caaaggtcca ggcttttggg ggctttttga ctgcaatggt catccccaat attggtgctt
    28501 ttattgcctg gggttttatt actgcgttat ttattcccac cggttggctg cctaacgaac
    28561 atttcgccaa aattgtcggc ccgatgatta cctatttatt gcccgtgatg attggttcta
    28621 caggtggtca tctggtcggc ggtaaacgcg gggcggtcat gggcggaata ggtactattg
    28681 gtgtgatcgt tggcgcagag atcccgatgt tccttggctc aatgatcatg gggccgctcg
    28741 gtgggttggt cataaaatat gtcgataagg cactggaaaa acgcatacct gccggttttg
    28801 agatggttat caataacttc tcattaggta tcgcggggat gctcctttgt ctgctgggtt
    28861 ttgaagtgat cggcccggcg gtgttaattg ccaatacttt cgtcaaagag tgtatcgagg
    28921 cgctggtaca tgcgggttat ctgcctctgc tgtcagtcat caatgaaccg gcgaaagtgc
    28981 ttttccttaa taatgcgatc gatcagggcg tctattatcc gctgggaatg caacaggctt
    29041 cggttaacgg taaatccatc ttctttatgg tggcctctaa cccaggtccg ggcctggggc
    29101 tgctgctggc gtttaccttg tttggtaaag ggatgagtaa acgttctgcg cccggggcga
    29161 tgattattca cttcctcggt gggatccacg aactgtattt cccatatgtg ctgatgaagc
    29221 cgctgactat tattgccatg attgcgggcg gtatgtctgg tacctggatg tttaacttac
    29281 tggacggtgg tctggtggct ggcccaagtc tggggtctat ctttgcttac ctggcactga
    29341 cgccgaaagg ctcgttcctg gcgacaattg ccggtgttac ggtaggtacc ctggtgtcct
    29401 ttgctattac ttcgctgata ctgaagatgg aaaaaacggt ggaaacggag agcgaagatg
    29461 agtttgctca gtcagccaat gcggttaagg cgatgaaaca agagggtgca ttctcgttaa
    29521 gcagggttaa gcgtatcgcc tttgtttgcg atacggggat gggctccagt gcgatgggcg
    29581 cgaccacctt ccgtaaacgc ctggaaaaag cggggctggc aatagaagta aaacattacg
    29641 cgatagaaaa cgtgcctgcg gatgccgata tcgtcgtcac tcacgccagt ctggaagggc
    29701 gcgtgaaacg tgtgacggat aaaccgctga tattgattaa taactatatt ggcgatccaa
    29761 aactcgacac tttatttaat caattaaccg ccgaacataa acactgatta cagaggtaaa
    29821 aatgaaaacc aaagttgctg ctatttatgg caagcgggat gtccgtctgc gcgaatttga
    29881 actgccagaa attaccgata atgaactgtt agtgagtgta atttctgaca gcgtctgttt
    29941 atcgacctgg aaagcggcgt tactcggtag tgaacataaa cgcgtacccg acgatttaga
    30001 aaatcatccg gtcattaccg ggcatgaatg tgccggggtt attgtcgaag tgggtaaaaa
    30061 tctcactggc aaatataaaa aaggccagcg ttttgtattg caaccggcga tggggttacc
    30121 aagcggatat tcagcaggct acagctacga atattttggc ggtaacgcca cctatatgat
    30181 tattcccgaa atcgccatta atttgggctg cgtattaccg tatcacggct cttattttgc
    30241 tgcggcgtcg ctggtagagc ctatgtgctg cattattggt gcttatcatg ccaattatca
    30301 caccacgcaa tatgtttatg agcatcgcat gggcgtcaaa cctggcggca atattgcact
    30361 gctggcgtgt gcgggtccga tgggcattgg cgctatcgat tacgccatta acggtggcat
    30421 acaaccgtca cgggtggtgg tggtcgatat cgacgacaaa cgcctggcgc aggtgcagaa
    30481 gctgctgccg gtggatctgg cggccagtaa aggcattgag ctggtgtatg tgaataccaa
    30541 agggatgagc gatccggtcc agacgctgcg ggcgctgaca ggagatgtcg ggttcgatga
    30601 catttttgtt tatgcggcgg tgcctgctgt cgttgagatg gctgacgaat tactggcgga
    30661 agatggctgt ctgaacttct ttgccgggcc gacggataaa aacttcaaag tgccgtttaa
    30721 tttctacaac gtccattaca acagcacgca cgtagtcggt acatccggcg gttcaacgga
    30781 cgacatgaaa gaggcgattg cgttaagcgc cacggggcag ttgcagccgt cctttatggt
    30841 cacgcatatc ggtgggctgg atgcggtgcc agataccgtg ctcaatctgc cggatatccc
    30901 tggcggtaaa aaactcattt ataacggcgt gaccatgccg ctcactgcca ttgccgattt
    30961 tgccgaaaaa ggcaaaaccg atccgctatt taaagagttg gcgcggctgg ttgaggaaac
    31021 gcacggcatc tggaatgaac aggccgagaa atatctgctg gcacaatttg gcattgatat
    31081 cggggaggcc gcgcaatgat gtccctggcg tggccattat ttcgcgttac ggaacaggca
    31141 gcgttggctg cctggccgca aaccggatgt ggcgacaaaa ataaaattga tggcctggcg
    31201 gtcaccgcga tgcgccaggc attaaacgac gtcgcttttc gtggacgagt ggttatcggc
    31261 gaaggagaaa ttgaccatgc gccgatgctg tggattggcg aagaggtggg caaaggcgat
    31321 gggccagaag tcgatatcgc ggttgatccc attgaaggca cgcggatggt ggcgatgggg
    31381 cagagcaatg cgctggcggt catggctttc gcccctcggg atagcctgtt acatgcgccc
    31441 gatatgtata tgaaaaagct ggttgttaat cgactggctg ccggggtgat tgatctgtct
    31501 ttgcctctgg cggacaactt gcgtaacgtg gcgcgggcgt taggtaaacc gctggacaag
    31561 ctgcgcatgg ttacgctgga taaaccgcgc ctgagtgccg cgattgagga agcaacacag
    31621 ctgggcgtga aggtttttgc cctgccggat ggcgatgtcg ccgccagcgt gctgacctgc
    31681 tggcaggata atccttacga tgtgatgtac accatcggcg gtgcgccaga aggcgtgatt
    31741 tctgcctgtg ccgttaaagc attaggcggg gatatgcagg ccgagctgat tgacttttgt
    31801 caggctaaag gggattacac ggaaaatcgg cagattgcag agcaggagag caagcgttgc
    31861 aaggcaatgg gcgttgacgt taaccgtgtg tactcgctcg atgaactggt aagggggaac
    31921 gatatcctct ttagcgctac gggcgtgacg ggcggcgagt tggtgaacgg tatccaacag
    31981 acggcgaatg gggtgcggac gcagacatta ctgatcggcg gcgcggacca aacgtgtaat
    32041 ataatagact ccctgcattg atggtgatct atcggaagac caatggcgaa gctgacagaa
    32101 gatgatgtgc ttgagcaact ggatgcacag gacaatttat tttcatttat gaaaactgcg
    32161 cattctattt tgctccaggg gatacgccag tttctgccgt cgctgtttgt cgataacgat
    32221 gaagagatcg tggaatatgc agtgaagccg ttactcgccc aaagcggccc gcttgacgat
    32281 attgatgttg cgctgcgttt gatttatgcg ctggggaaaa tggataaatg gctgtacgcc
    32341 gatatcacgc atttttccca gttctggcat tacctgaacg aacaggatga aacgcccgga
    32401 tttgccgatg acatgacctg ggattttatc agcaacgtca atagcataac ccgcaatgcg
    32461 atgctctatg acgcgttgaa agcgatgaag ttcgccgatt tcgccgtctg gtcagaggcg
    32521 cgttttagcg ggatggtcaa aacggcgctg acgctggcag taacgacaat cttaaaggaa
    32581 ttaacgccgt gaaaattgaa ttaacggtga atgggctgaa gatacaggca cagtaccaga
    32641 acgaagagat tgaaaatgta cataaaccgt tgctgcgtat gctggcggcc ttacagacgg
    32701 taaatccgca gcggcgtacg gtggtttttc tttgcgctcc gccggggacg ggtaaatcta
    32761 ccctaaccac cttctgggaa tatctcgcgc aacaagaccc ggagctgccc gctattcaga
    32821 cgctcccgat ggatggtttt caccattaca atagctggct ggacgcgcat caattgcgcc
    32881 ccttcaaagg cgcaccagag acatttgacg ttgcgaaact ggcggaaaat ctgcgccagg
    32941 tggtggaagg ggattgtacg tggccgcagt acgatcgaca aaagcatgat cctgttgaag
    33001 atgcgttgca cgttaccgca ccactcgtca tcgtcgaagg aaactggttg ttgctggacg
    33061 acgagaagtg gtgccagctt gcgcaatttt gtgatttctc gatctttatc aacgcgccag
    33121 ctactgcgtt gcgggagcgt ctggtggggc gcaagctggc gggaggggta tcgctggcag
    33181 atgcagaagc cttttatgac cgtaccgacg ggccgaatgt tcgccgggtg ctggaagaga
    33241 gtctgcctgc aaatttgacc ttaatgatga ccgctacggg tgaatatcgt ctggtggatt
    33301 aacgttcctg attacaccat cttccagtcg caccacacga gagaaatgac gccgggtaaa
    33361 tgcggcgtca tgactgattg ctaccacgct cgtgcctcgc tggcggcact tctccagcca
    33421 gctttcaaac acgctcaacc aattttcgtc aaaatcccga ctcggctcgt ccagcagtaa
    33481 tagtggcgga gagagtgctt ccaggcaggc gacggcgacc atccgtcgct gcgcactatg
    33541 cagatccaac gggtgcgagt tggcagtgtc tgctaactga caacattgca gggcagcatg
    33601 ggtacgttga gtgatttcat ccgccgggca tttttgcagt ttcaaaccaa atgcgatctc
    33661 gtcagcgacg gtgctgtgaa aaagttggtt ttccgcttcc tgaaacagta cgccaacctt
    33721 tgccgcgcgc tggcggtttt tcaggttttt catcgcctgt tgttgcagca taaccgtacc
    33781 ggctgtgggc gtaagaagac ctgccattac tcgtaacagc gtcgatttcc ctgcgccatt
    33841 gtcaccggtc agcgccaacc attcaccttg tttgagttgc agagaaatgt cgcaaagaca
    33901 atccgttgcc gcgccaggcc agcgataaga tatctgattt aacgttaaca tagtggctgg
    33961 agcgctccgt tttgcaattg ccacgcttgc tggcagtagt tcaggaaagg tgtgcgatga
    34021 cgttcaaaaa gaataatcag cgaaccacgt tcaaacgccc agtgttgtaa gcgctgcaac
    34081 agcatttcgc tggcctgcgg cgtcaggcgg ctaaaggctt catcaagaat taagagcttt
    34141 ggctgcatcg cgatggcgca ggcgatgacc acgcgctgtg tctctccacc ggagagtgtt
    34201 gccgggtgac gatggcgcaa cggctggcac tcggtcagag tcagggcggc atcaatccgc
    34261 gccataatct ctgcttcagc aagacacaga ttttctggcc caaaggcaac ttcttcttcc
    34321 acgctaaagg tacagccgga gagctgcaaa tagggcgatt gctggacgag ctggatggtg
    34381 gcggattgtt cattaagcgg gagatgccca atgggcgtac cgaggagcgt ccccgtgcct
    34441 gttatctctc ctggcagaaa gtccggatac cagcccgcca tcagctgtgc cagcgtactt
    34501 ttgccactgc cgttgtcacc aaagatggcg accatccccg gtttgacata gtgaaagtca
    34561 taacagaacg gaggatgcgt tgagtgtgtc gggcaataac ggaactgttc taacgtaacc
    34621 atatccacgc tccgaattct gtcagcatca gcaagatcat ggtgtaacgc gctacgcgtt
    34681 gtagggtgct atcggctggt gcccacaacg ttgttcgtcg gttgtggatc cgaaatgccc
    34741 gcatatccag cgccgcacca cggacagcca gatcgttaag cgcattgtgt gttagcggaa
    34801 taattaacgc gggcatcgca cgtaaacgct gataccagcc ctcatccagc ggcacgccgc
    34861 gggcacgctg cgcttcatga ataatcgcta attgccgttt taactgctca acgaccaata
    34921 acggcccggc aaacagataa gcgacgcctg gcggcaggcg agaagcaaac agagcgcgaa
    34981 taaaacattg cacggggacg tactgcatcc acaactgtga ggttgaaaca atcgccagaa
    35041 tccgcaacca gagcgtaata gcatgcgacc accgttctgg cgagcgtggc gtgccgctta
    35101 gccattctgg tagccagcca ccgtgtacca gccacagccc ggctcccagc gagaacatca
    35161 gccagacgac atattttgcc cgccgccgcg tggcctttag cgcaataagg cagaaaaagg
    35221 tcgccgcgct atagatcggc aaaatggtct gcgcgggcag gataagcgtg gtgcaggccg
    35281 ccaatgccca cagtattaat gaggtaaatg gatgcattta gcgcacggca gccattgccg
    35341 gaaaatggcg cgtggtgcga atcggcagct ggcgcagtaa aagccagaca atcaccgctg
    35401 taagaatttt atcgaccagg ttcgcgccta tcaccgtaat cgccaccgac tccaccaggt
    35461 tttgccccat cgagtgcatc caggcaacga acaaatcggc tccgctaccg gtgacaccgc
    35521 cgaacagcgc agtacgcagc ggtacagcga cgaccgttac cgcaagcgta ataatcacgc
    35581 cgctgacaac gacttttggc agggtgcgaa accaacccgc tcgcgccagc caaccggcga
    35641 ccaggccgat gaccatcgcg acgggtgcaa atgcggcggc gatcgggtcg gtcagcaatc
    35701 cccagagtaa attggtcagt aagccggtca gcattccgat caccggacca agcagtaccg
    35761 cgctaataag tgtgccgatg gagtcgagaa atataggtaa tttgaccatg ctggccagtt
    35821 gcccgccgat catattgatg gcgatagata taacgatcaa caccagtgcc tgactggaaa
    35881 agtggctacg cgccataaga aaacctcttt gaaagtagtt atttaacggg ggaaatatgc
    35941 aactcgtcgt aaccagaaag cgcgcaaggt tggcggctaa acgccagttc ctcaaggcta
    36001 ccgatcacca gttcaagcgt ggtccgcacg gtacagccgg gcatcatatg accgccaagc
    36061 atcgtgccgt gtggatcgga aacgcagaga tgaagatgct caccactctg ttccagcgtg
    36121 ccgtttagtg agatcacttc gaatgtaccg ttcaggagcg tggtgccttc ttgcctggca
    36181 tagcgcaagg cgacgtcagt caggctaccg gtacagcctg caatccaggc ggcatgaagt
    36241 tgatgctgct gtgcaaaagc gtgtagctgg gaaaaaactt cctgcccggg gaggaggcgc
    36301 agtgcgtaaa agcgtgcgga ggaagaatat gggtagggca atgtcatcat tggagtactc
    36361 cgaaactcgg acgtattgcg gataatatcg acgctgcatc agtcatattc tgatataagt
    36421 cagggtttgt gacggcacaa cgaaaagatt ttgctacgct tattcgtgat tctgcaaagt
    36481 ggaataaatt ctacaggggc ggggctgaaa acgtgacgta agtcagataa ttgctttctt
    36541 tgactggaca atcattcctt ttattccacg tttcgcttat cctagctgaa gcgtttcagt
    36601 cgattaaatg ttcgacaatt aaccaatcag tcgcagtttg cgacaggtaa ggtttccccg
    36661 gacgatttgc tggattactc tgtctggcaa atttgctcag ggaaaacctt gcaggagatc
    36721 tatgaccgta cgcgtagcga taaatggctt cggtcgcatc gggcgtaatg tggttcgtgc
    36781 tttgtatgaa tccggacgcc gggcggaaat taccgtggtg gcaatcaacg aactggcgga
    36841 tgctgcgggc atggcgcatt tgttgaaata tgacaccagc catggccgtt ttgcatggga
    36901 agtacgacag gaacgcgatc aactttttgt tggtgatgac gccatccgcg tattgcatga
    36961 acgttcactg caatcgctcc cctggcgtga acttggcgtt gatgtagtcc tcgactgcac
    37021 cggcgtatat ggctcccgcg agcatggcga agcgcatatt gccgccgggg ccaaaaaagt
    37081 gctcttttca catcctggca gtaacgatct cgacgcgacc gttgtttacg gcgtcaatca
    37141 ggatcaactt cgtgcggaac accgcatcgt ttctaacgct tcctgtacca cgaattgcat
    37201 aattcccgtc atcaaattgt tagatgatgc gtacggtatt gagtccggca ctgtgaccac
    37261 aattcactcc gccatgcacg atcaacaggt tattgatgca taccatcctg acctgcgtcg
    37321 cacccgggca gccagccagt cgatcattcc ggtcgatact aaactggccg ccggtatcac
    37381 acgatttttt ccgcaattta acgatcgctt tgaagcgatt gcggtacgtg tgccaaccat
    37441 aaatgtgacg gcaatcgatt taagcgtgac ggtgaagaaa cctgtaaaag ccaatgaagt
    37501 caacctgttg ctgcaaaaag cagcacaagg tgcatttcat ggtatagttg actatacgga
    37561 attgccgttg gtctctgtag attttaacca cgatccgcac agtgccattg tcgatggcac
    37621 ccaaacccgg gtcagtggcg cacacctgat caaaacgttg gtctggtgcg ataacgaatg
    37681 gggctttgct aaccgaatgc tcgacacgac gttagctatg gctactgttg ctttcaggta
    37741 agacgcaagc agcgtctgca aaacttttag aatcaacgag aggattcacc atgtctgtaa
    37801 ttaagatgac cgatctggat cttgctggga aacgtgtatt tatccgtgcg gatctgaacg
    37861 taccagtaaa agacgggaaa gtaaccagcg acgcgcgtat ccgtgcttct ctgccgacca
    37921 tcgaactggc cctgaaacaa ggcgcaaaag tgatggtaac ttcccacctg ggtcgtccta
    37981 ccgaaggcga gtacaacgaa gaattctctc tgctgccggt tgttaactac ctgaaagaca
    38041 aactgtctaa cccggttcgt ctggttaaag attacctcga cggcgttgac gttgctgaag
    38101 gtgaactggt tgttctggaa aacgttcgct tcaacaaagg cgagaagaaa gacgacgaaa
    38161 ccctgtccaa aaaatacgct gcactgtgtg acgtgttcgt aatggacgca ttcggtactg
    38221 ctcaccgcgc gcaggcttct actcacggta tcggtaaatt cgctgacgtt gcgtgcgcag
    38281 gcccgctgct ggcagctgaa ctggacgcgc tgggtaaagc actgaaagaa cctgctcgcc
    38341 cgatggtggc tatcgttggt ggttctaaag tatctaccaa actgaccgtt ttggactccc
    38401 tgtctaaaat cgctgaccag ctgattgttg gtggtggtat cgctaacacc tttatcgcgg
    38461 cacaaggcca cgatgtgggt aaatccctgt acgaagctga cctggttgac gaagctaaac
    38521 gtctgctgac cacctgcaac atcccggttc cgtctgatgt tcgcgtagca accgagttct
    38581 ctgaaactgc accggctacc ctgaaatctg ttaacgatgt gaaagctgac gagcagatcc
    38641 tggatatcgg tgatgcttcc gctcaggaac tggctgaaat cctgaagaat gcgaaaacca
    38701 ttctgtggaa cggtccggtt ggcgtgttcg aattcccgaa cttccgcaaa ggtactgaaa
    38761 tcgtggctaa cgctatcgca gacagcgaag cgttctccat cgctggcggc ggcgacactc
    38821 tggcagcaat cgacctgttc ggcattgctg acaaaatctc ctacatctcc actggcggcg
    38881 gcgcattcct cgaattcgtg gaaggtaaag tactgcctgc agtagcgatg ctcgaagagc
    38941 gcgctaagaa gtaaaaaatc acagggcagg gaaacctgcc cttgtttcag cgcgctttta
    39001 gagcacgcat tttttcatac tctaaataat tcgagttgca ggaaggcgac aagagagtga
    39061 atccccagga gcttacataa gtaagtgact ggggtgagcg aacgcagacg cagcacatgc
    39121 aacttgaagt atgatgagta taaggcccga cgatacagga caagagacat gtctaagatt
    39181 tttgatttcg taaaacctgg cgtaatcact ggtgatgacg tacagaaagt tttccaggta
    39241 gcaaaagaaa acaacttcgc actgccagca gtaaactgcg tcggtactga ctccatcaac
    39301 gccgtactgg aaactgctgc taaagttaaa gcgccggtta tcgttcagtt ctccaacggt
    39361 ggtgcttcct ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg tgctgctatc
    39421 ctgggcgcga tctctggtgc gcatcacgtt caccagatgg ctgaacatta tggtgttccg
    39481 gttatcctgc acactgacca ctgcgcgaag aaactgctgc cgtggatcga cggtctgctg
    39541 gacgcgggtg aaaaacactt cgcagctacc ggtaagccgc tgttctcttc tcacatgatc
    39601 gacctgtctg aagaatctct gcaagagaac atcgaaatct gctctaaata cctggagcgt
    39661 atgtccaaaa tcggcatgac tctggaaatc gaactgggtt gcaccggtgg tgaagaagac
    39721 ggcgtggaca acagccacat ggacgcttct gcactgtaca cccagccgga agacgttgat
    39781 tacgcataca ccgagctgag caaaatcagc ccgcgtttca ccatcgctgc gtccttcggt
    39841 aacgtacacg gtgtttacaa gccgggtaac gtggttctga ctccgaccat cctgcgtgat
    39901 tctcaggaat atgtttctaa gaaacacaac ctgccgcaca acagcctgag cttcgtattc
    39961 cacggtggtt ccggttctac tgctcaggaa atcaaagact ccgtaagcta cggcgtagtg
    40021 aaaatgaaca tcgataccga tacccaatgg gcaacctggg aaggcgttct gaactactac
    40081 aaagcgaacg aagcttatct gcagggtcag ctgggtaacc cgaaaggcga agatcagccg
    40141 aacaagaaat actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc gatgatcgct
    40201 cgtctggaaa aagcattcca ggaactgaac gcgatcgacg ttctgtaaga tattcctttc
    40261 tgcttatctc aaggcccgct ctgcgggtct ttttttcgcc aaaagcaaaa ttgcctgatg
    40321 cgctacgctt atcaggccta cacgtatcct gcaatatatt gaatttgcag gtttttgtag
    40381 gccggataag gcgttcacgc cgcatccggc atgacaaagc gcacattgat atctgtctga
    40441 gccccgctct gcggggattt tttcgccaaa agcaaggtga ttcagatgag aaatctgtga
    40501 cctatttggc aaaattatgc tttattgttt acccttgtca gactgcccgt cataaggcgg
    40561 cggagtgtat ttctccattt tgagtcagtt gaaaaggaat attgaatgga agatttgaat
    40621 gttgtcgata gcataaacgg cgcgggaagc tggctggtag ctaaccaggc gctgctgcta
    40681 agttatgcag taaacatcgt ggcggcactc gcgatcatca tcgttggttt gattatcgcg
    40741 cggatgattt ccaacgcggt gaatcgcctg atgatctccc gtaaaatcga tgccactgtt
    40801 gctgattttc tttctgcatt agtccgttac ggtattatcg cctttacgct aatcgctgcc
    40861 ctgggacgcg tgggtgtaca aacagcgtca gtcattgctg tactcggtgc cgcaggctta
    40921 gctgttggtc tggctttgca ggggtcactt tctaacctgg ccgctggcgt gttacttgtc
    40981 atgttccgac cgttccgtgc cggagaatat gttgacctcg gcggcgtagc cggtactgtg
    41041 ctgagtgtgc agattttctc caccaccatg cgtactgcag acggtaaaat tatcgttatt
    41101 ccgaacggta aaattattgc cggaaatatt attaacttct cccgcgagcc agttcgccgt
    41161 aacgaattta ttattggcgt ggcgtatgat tccgatatcg atcaggttaa gcagatcctg
    41221 accaatatta tccagtctga agatcgcatt ttgaaagatc gcgaaatgac tgtgcgcctg
    41281 aacgaacttg gtgcatcgtc gattaatttc gtggtccgcg tctggagcaa cagcggcgat
    41341 ctgcaaaacg tgtactggga tgtgctggag cgtattaaac gtgaatttga tgccgccggt
    41401 atcagcttcc cgtacccgca aatggatgtg aactttaagc gggtgaaaga agacaaagct
    41461 gcgtaatcaa cgctgaggcc agataatact ctctggcctc tctcttatta gttattctga
    41521 ttgccaatta atattatcaa tttccgctaa taacaatccc gcgatatagt ctctgcatca
    41581 gatacttaat tcggaatatc caacgtgttt tcttattact ttcaaggtct tgatcttggg
    41641 gc
//
LOCUS       DAJTOR010000011        40598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTOR010000011 DAJTOR010000000
VERSION     DAJTOR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.011"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(239..1096)
                     /gene="murI"
                     /locus_tag="OO249_000571"
     CDS             complement(239..1096)
                     /gene="murI"
                     /locus_tag="OO249_000571"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5634979.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1041..2889)
                     /gene="btuB"
                     /locus_tag="OO249_000572"
                     /pseudo
     CDS             complement(1041..2889)
                     /gene="btuB"
                     /locus_tag="OO249_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2943..3133)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3247..4347
                     /gene="trmA"
                     /locus_tag="OO249_000573"
     CDS             3247..4347
                     /gene="trmA"
                     /locus_tag="OO249_000573"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5634980.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4387..4746)
                     /locus_tag="OO249_000574"
     CDS             complement(4387..4746)
                     /locus_tag="OO249_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5634981.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4746..5396)
                     /gene="fabR"
                     /locus_tag="OO249_000575"
     CDS             complement(4746..5396)
                     /gene="fabR"
                     /locus_tag="OO249_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5634982.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5717..7117
                     /gene="sthA"
                     /locus_tag="OO249_000576"
     CDS             5717..7117
                     /gene="sthA"
                     /locus_tag="OO249_000576"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5634983.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7100..8017)
                     /gene="oxyR"
                     /locus_tag="OO249_000577"
     CDS             complement(7100..8017)
                     /gene="oxyR"
                     /locus_tag="OO249_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5634984.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8284..9657)
                     /gene="argH"
                     /locus_tag="OO249_000578"
     CDS             complement(8284..9657)
                     /gene="argH"
                     /locus_tag="OO249_000578"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5634985.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRLAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDAFDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9718..10494)
                     /gene="argB"
                     /locus_tag="OO249_000579"
     CDS             complement(9718..10494)
                     /gene="argB"
                     /locus_tag="OO249_000579"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5634986.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10502..11506)
                     /gene="argC"
                     /locus_tag="OO249_000580"
     CDS             complement(10502..11506)
                     /gene="argC"
                     /locus_tag="OO249_000580"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5634987.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11660..12811
                     /gene="argE"
                     /locus_tag="OO249_000581"
     CDS             11660..12811
                     /gene="argE"
                     /locus_tag="OO249_000581"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5634988.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDIDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVIERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            13163..15814
                     /gene="ppc"
                     /locus_tag="OO249_000582"
     CDS             13163..15814
                     /gene="ppc"
                     /locus_tag="OO249_000582"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5634989.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEAPPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYKSWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYL
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15997..17730
                     /locus_tag="OO249_000583"
     CDS             15997..17730
                     /locus_tag="OO249_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5634990.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17945..18796
                     /locus_tag="OO249_000584"
     CDS             17945..18796
                     /locus_tag="OO249_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5634991.1"
                     /translation="MYHDISYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18783..19124)
                     /locus_tag="OO249_000585"
     CDS             complement(18783..19124)
                     /locus_tag="OO249_000585"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5634992.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19126..20004)
                     /locus_tag="OO249_000586"
     CDS             complement(19126..20004)
                     /locus_tag="OO249_000586"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5634993.1"
                     /translation="MTSSAGQRISCNVVETRLNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19970..22267)
                     /locus_tag="OO249_000587"
     CDS             complement(19970..22267)
                     /locus_tag="OO249_000587"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5634994.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTPGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22318..22638)
                     /locus_tag="OO249_000588"
     CDS             complement(22318..22638)
                     /locus_tag="OO249_000588"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5634995.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22653..23732)
                     /locus_tag="OO249_000589"
     CDS             complement(22653..23732)
                     /locus_tag="OO249_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5634996.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            24041..26542
                     /gene="ptsP"
                     /locus_tag="OO249_000590"
     CDS             24041..26542
                     /gene="ptsP"
                     /locus_tag="OO249_000590"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5634997.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSSILEAHRSLAGDTSLRQHLLTGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMTQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26554..27216
                     /gene="fsa"
                     /locus_tag="OO249_000591"
     CDS             26554..27216
                     /gene="fsa"
                     /locus_tag="OO249_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5634998.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27227..28330
                     /gene="gldA"
                     /locus_tag="OO249_000592"
     CDS             27227..28330
                     /gene="gldA"
                     /locus_tag="OO249_000592"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR5634999.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAAFSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            28604..29221
                     /locus_tag="OO249_000593"
     CDS             28604..29221
                     /locus_tag="OO249_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR5635000.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29248..30153)
                     /gene="yijE"
                     /locus_tag="OO249_000594"
     CDS             complement(29248..30153)
                     /gene="yijE"
                     /locus_tag="OO249_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5635001.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMHPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(30247..32427)
                     /gene="katG"
                     /locus_tag="OO249_000595"
     CDS             complement(30247..32427)
                     /gene="katG"
                     /locus_tag="OO249_000595"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5635002.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     SSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32756..33646)
                     /gene="metF"
                     /locus_tag="OO249_000596"
     CDS             complement(32756..33646)
                     /gene="metF"
                     /locus_tag="OO249_000596"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5635003.1"
                     /translation="MSFFHASQRDALNQRLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33995..36427)
                     /gene="metL"
                     /locus_tag="OO249_000597"
     CDS             complement(33995..36427)
                     /gene="metL"
                     /locus_tag="OO249_000597"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5635004.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36430..37590)
                     /gene="metB"
                     /locus_tag="OO249_000598"
     CDS             complement(36430..37590)
                     /gene="metB"
                     /locus_tag="OO249_000598"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5635005.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37867..38184
                     /gene="metJ"
                     /locus_tag="OO249_000599"
     CDS             37867..38184
                     /gene="metJ"
                     /locus_tag="OO249_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5635006.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38368..38976
                     /locus_tag="OO249_000600"
     CDS             38368..38976
                     /locus_tag="OO249_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5635007.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            39121..39796
                     /locus_tag="OO249_000601"
                     /pseudo
     CDS             39121..39796
                     /locus_tag="OO249_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(39883..40203)
                     /locus_tag="OO249_000602"
                     /pseudo
     CDS             complement(39883..40203)
                     /locus_tag="OO249_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40205..40598)
                     /locus_tag="OO249_000603"
                     /pseudo
     CDS             complement(40205..40598)
                     /locus_tag="OO249_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
BASE COUNT         9982 a        11155 c        10098 g         9363 t
ORIGIN      
        1 taaagagcag tgccgcttcg ctttttctca gcggcgcggg gtgtgcataa tacgctttcc
       61 cgctacagag tcaagcattt atttttgctt tctctgtcgg aattctcagg agaaccccgc
      121 tgacccggcg gcgtgtttgc cgttgttccg tgtcagtggt ggcgcattat agggagttat
      181 tccggcctga caagaggaaa tttaaaataa ttttctgacc gcgcaacatt caaccaaatc
      241 agcctaaaac tgccagtttt tcgagcgttt cgaagccgta acgctgtaaa acgggcaata
      301 attgttcagc ttctggcgtc attgccatac aaaaggcaat attcgcatcg gcagattttg
      361 catccggggc ttcatgttct aacaaccagg ccgttcggcg agcaatcgct gcgccagaat
      421 ccactagccg ggttccctct ggcagcactt gtaacagttc ttcttgtagt agagggaaat
      481 gggtgcaacc caatacaacg gtatctggcg gctctttcat tcttaaccac gggcgtagga
      541 tacgttttag tgcatccaga gaaacatctt cgccatgtag cttcgcttca gccaactcaa
      601 ccatctctgc cgagcccagc atttctatct ggcattcatt agcgaaacgc gcgatcagct
      661 catgagtata agaacgttta actgttccgc gggttgccag taatccgaca atgccatttg
      721 ccgtcagacg tgcagcaggt ttaatcgccg gcacgacacc aacaaccggg aagtcgaact
      781 tttcgcgtaa tgcaggaagt gaaacggtac tggcagtgtt gcaagcgacc acagccagcg
      841 caaggggata acgttcttgc accgcggtga caattgccac cactcgctca acaataaacg
      901 cttcgctttt ttcgccatac gggaaagcga cgttatcgaa agcataaatg taatggagat
      961 ccggtaagag atgccggatc tcgtcataga ccgacaaccc accgacgccg gagtcaaaca
     1021 ccagcacggt gggacgtggt tcagaaggtg tagctgccag acaaggtgta ttcccgtcct
     1081 gcagtttggt agccatagac tgtctcataa tctttgtcga acaggttggc tattttacca
     1141 cgaactgtca ggtgagaggt gaccggatac gcaaccgcaa gatcccacaa gctcacaccg
     1201 cccattttaa cggtttgata aggataagat gagtaatcct tatcatagcg agtgcctaaa
     1261 tactgataag taatacccca gtcgaagtca tacaactgcc agtcgagctg gtatttcacc
     1321 tgctgtttag cacggcgtaa caacggcgtg tcggtaatcg cattgcgcgc atcgacataa
     1381 tcataactca cagtatgcgt cagtggtccg gtatcaaaat tggcggtcgc ctcgacgccc
     1441 ttaatccgcg ctttcccttc gttgtaatat ttcagggtgt gatcatcata atcgatcaag
     1501 tcactgacat cgttacgata tccggaaata cgccagttca ccccagcggt taagccttca
     1561 aacgcgcctt cccactgttt gcttttctcc gggtccagat tcggatttcc gtagaagcca
     1621 tacagttgcc ccagatttgg tgccttataa gatgtcccgt aggaagcaat gaagcgataa
     1681 ccttcgatga attcccaacc ggcgctggtt tgccaggttc catgacgacc aaactgtgag
     1741 ttatcgtcac tgcgtgctgc gccttcaaag gtaaaatcgc cgacttgttg cagcccggtc
     1801 agatagatgc cggtattacg ttgatcatat ccatcctcaa cataacctgt acccggcgtc
     1861 gtagtctgtt tctgccagtc gacacccgca ccaatactac cgtgaccaac gatgacattg
     1921 tttgcccact ggacggtgta ttgcttcatc tcatcgagcg tcgccgacga atcataacga
     1981 ccataatggg gatcgtagtt gtaatctttg ctatggctat agctggtaat gagttgtgat
     2041 ttaatcagtt cgccgttata gcgcagcccg gcgtcccaac tttggctata gagtttacgg
     2101 atatcgagca acggtgaacc gggagaataa tacgcgtcat aattggtacg gttatcatag
     2161 ccatagccgc gcacaaagcc gctccaggca tcagtaaagt tatgctccag cgcgccataa
     2221 agcgttttac ttaaaaaaac atcgttatct gtctgcgctt gcgttccggt attaccatag
     2281 gcaacaacat cataaccatg agtatgggca taatcgccca acagcgttac ccgtgtctta
     2341 tcccccagtt gttgctgcgt agagacatca tagttctgat aactattgct tccccaccct
     2401 gctgaaattt ccgttccggg gtcatcgcgc gtcgtgatga tattcaccac cccgcctatt
     2461 gcatcggaac cataaacagc ggagcgcggc ccacggatat attcaacacg ctggacaagc
     2521 gcacgcaata gggaactggc taaggtcggc agaaccactc acccccgcca gattcaggcg
     2581 tacgccatca attaacacca acacatgact ggcatttgta ccgcgaataa aaatagatga
     2641 gagctgacct gaaccgccgt tttgggtgat atcgacgccc ggaagacggc gcagcacatc
     2701 attgaccgag gtcgactgcc agcggtcgat atcctgacgg gtcacaacgg tggttggtgc
     2761 aagcacagtg ctgcgcggct gttcaaaacg gttagcagta acgacgagag tatccgggct
     2821 ggtatcctgt gcccaagcgg aaaaggctgt gacagaacac gccgtcagca gcgaagcttt
     2881 tttaatcatt gtaaagcatc cacaatagaa gaaggatgcc gcaggtttca tcaatattac
     2941 gcgatgatga gacgttggcc ggcaggtctt cgggcttgga ggggtatcta agatactaag
     3001 agatgatgac ttcccaccgg atggcagtgt ccgcataacg caatcatcgc acctttcctt
     3061 accgctgcgc gtcagctcca gattcgcact ggattcccta ttaactcaca ggaccggcaa
     3121 gtggatgcta caggttgtaa caagttactg tccagacgta gctcacaaat aggaattcat
     3181 caagatctgg acatctgatg agcaatccct acaatcgccg cgtactttaa tttttcagga
     3241 tacatcatga cccccgaaca ccttccaaca gaacagtatg aagcgcagtt agccgaaaaa
     3301 gtggtacgtt tgcaaagtat gatggcaccg ttttctgacc tggttccgga agtgtttcgc
     3361 tcgccggtca gtcattaccg gatgcgtgcg gagttccgca tctggcacga tggcgatgac
     3421 ctgtatcaca tcattttcga tcaacaaacc aaaagccgta tccgcgtgga tagcttcccc
     3481 gccgccagtg aacttatcaa ccagttgatg acggcgatga ttgcgggtgt gcgtaataat
     3541 cccgttctgc gccacaagtt gttccagatt gattacctca ccacgctgag taatcaggcg
     3601 gtggtttccc tgctatacca taagaagctg gatgatgagt ggcgtcagga agcggaggcc
     3661 ctgcgcgatg cactgcgcgc gcagaatctg aatgtgcatc tgattggtcg ggcaacgaaa
     3721 accaaaatcg agctggatca agattacatc gacgagcgtc tgccggtcgc agggaaagag
     3781 atgatctacc gtcaggtaga aaacagcttt acccagccga acgcggcgat gaatattcag
     3841 atgctggaat gggcgctgga cgtaaccaaa ggctcaaaag gcgatttact ggagctgtac
     3901 tgcggcaacg gtaacttttc attagcgctg gcgcgcaatt ttgatcgggt attagccacc
     3961 gaaatcgcta agccgtcggt tgctgctgcg caatacaaca tcgcagctaa ccatattgat
     4021 aacgtacaaa ttattcgtat ggcggcagaa gaatttactc aggcgatgaa tggtgtgcgc
     4081 gagtttaacc gcctgcaagg gatcgactta aagagttatc agtgcgaaac catttttgtc
     4141 gacccaccgc gcagcggtct ggacagtgaa accgagaaaa tggtgcaggc gtatccgtgt
     4201 attttgtaca tctcctgtaa cccggaaacg ttatgcaaga atctggaaac attaagccag
     4261 acgcacaagg tcgaacgtct ggctctgttt gatcagttcc cctacacgca ccatatggag
     4321 tgcggcgtat tactgaccgc gaagtaaaac ccatgccgga tgcgccagca tccggcataa
     4381 taccgattac tcagcaactt cctgcttacg gttacgcatc ttcgcgccaa tccagaacac
     4441 cataatgacg gagagtactg ccgggaagaa gttagagccg atatccggat attccgcacg
     4501 aactaccgtg ctgtacagca gtacgccgag aataaaacag gcagctgcca gacccggcaa
     4561 gcctaccggc atagtgcgat taagataacg ttgatgcagg cagtaaaccg tcagcaccag
     4621 ggaaataatc gggaatacag aaaatggcac aatggagcta aacagcgctg cgaaagtacc
     4681 attaatcgat aagccagcaa ctaacgccag cagcagcgta cctctatctt gatttgcttg
     4741 tttcattact cgtccttcac atttcccgga ataattgcgg ttttctcttg ttcacggcga
     4801 taccagtaat aagccccttt cgaaatcatt cgcagttgca gtaccagtcg ctcttctaat
     4861 tgccgacgtt gttcgacgcc gacgtccaac gcctcggcac ccgcactgaa gacaattgtc
     4921 accattgctt cggcttgcgc ttcagtaaac gcacgcggca tatggttttc gagttccaga
     4981 tagtccgcaa gttccgcaat gaagtgctga atttcacgcg caacggcggc acgaaacgca
     5041 gcggaggtgc cggagcgttc ccgcaataat aaccggaagg cgttaggatt attaccgatg
     5101 aactccataa atgtggagac cgaggtgcgg atcacactcc cgcctttggc gatacgctga
     5161 cgcgcctggc gcatgagttg gcgtagcatt aaaccgctct catcaaccat ggtcagaccc
     5221 agttcgtcta cgtcgcggaa atgccgataa aaagaggtgg gagcaatgcc cgcttcacgc
     5281 gccacttcac gcaaactcag gctggcgaag ctgcgttcag cacttaattg gctaaatgcg
     5341 gcttccacca gcgaacggcg ggttttttct ttttgttgcg ctcttatgcc catcacgatg
     5401 tctgaatcct tgccaaaagt actgctggca ctataccaga gaatgaacat actggattgc
     5461 tccagtattg tgaatgaacg gtaacgcaat aaaacatatt tactggccgg ttcttttacg
     5521 tacagcggaa acctgccgct taaacggaga gtatcgtcga taaaaatcca ataaaacgtc
     5581 agggcaaaag taagaaacag acaaagcaaa ggccgctcag gatatagcca gataaatgac
     5641 ggggatcaat tggcttaccc gcgataaaat gttaccattc tgttgctttt atgtataaga
     5701 acaggtaagc cctaccatgc cacattccta cgattacgat gccatagtaa taggttccgg
     5761 ccccggcggc gaaggcgctg caatgggcct ggttaagcaa ggtgcgcgcg tcgcagttat
     5821 cgagcgttat caaaatgttg gcggcggttg cacccactgg ggcaccatcc cgtcgaaagc
     5881 tctccgtcac gccgtcagcc gcattataga attcaatcaa aacccactat acagcgacca
     5941 ttcccgactg ctccgctctt cttttgccga tatccttaac catgccgata acgtgattaa
     6001 tcaacaaacg cgcatgcgtc agggatttta cgaacgtaat cactgtgaaa tattgcaggg
     6061 gaacgctcgc tttgttgacg agcatacgtt ggtgctggat tgcccggacg gcagcgtcga
     6121 aacactaacc gctgaaaaat ttgttattgc ctgcggctct cgtccatatc atccaacaga
     6181 tgttgatttc acccatccac gcatttacga cagcgactca attcttagca tgcaccacga
     6241 accgcgccat gtacttatct atggtgctgg agtgatcggc tgtgaatatg cgtcgatctt
     6301 ccgcggtatg gatgtaaaag tggatctgat caacacccgc gatcgcctgc tggcatttct
     6361 cgatcaagag atgtcagatt ctctctccta tcacttctgg aacagtggcg tagtgattcg
     6421 tcacaacgaa gagtacgaga agatcgaagg ctgtgacgat ggtgtgatca tgcatttgaa
     6481 gtcgggtaaa aaactgaaag ctgactgcct gctctatgcc aacggtcgca ccggtaatac
     6541 cgattcgctg gcgttacaga acattgggct ggaaactgac agccgcggac agctgaaggt
     6601 caacagcatg tatcagaccg cacagccgca cgtttacgcg gtgggcgacg tgattggtta
     6661 tccgagcctg gcatcagcgg cctatgacca ggggcgcatt gccgcgcagg cgctggtaaa
     6721 aggcgaagcc accgcacatc tgattgaaga tatccctacc ggcatttaca ccatcccgga
     6781 aatcagctct gtgggcaaaa ccgaacagca gctgaccgca atgaaagtgc catatgaagt
     6841 gggccgcgcc cagtttaaac atctggcacg cgcacaaatc gtcggcatga acgtgggcac
     6901 gctgaaaatt ttgttccatc gggaaacaaa agagattctg ggtattcact gctttggcga
     6961 gcgcgctgcc gaaattattc atatcggtca ggcgattatg gaacagaaag gtggcggcaa
     7021 cactattgag tacttcgtca acaccacctt taactacccg acgatggcag aagcctatcg
     7081 ggtagctgcg ctaaatggct taaaccgcct gttttaacac tttatcgaaa tggccatcca
     7141 ttcttgcgcg gatggcctct gccagctgct catagcggct gcgcagcggt gagccaggac
     7201 gataaaccag gccaatcgtg cggcgaggtt ccggcttaat acacggcaaa taaacaaccc
     7261 catcgcgttt gcgctccggc ggcacagcca gcgcaggcag taaagtgatc ccgctacctg
     7321 ccgccaccat gttacgcagt gtctccaggc tggtcgcgcg gaagtgtgta tcttcatccg
     7381 ccccggcttc aaagcagaaa cccattgcct gatcgcgcaa acagtgacca tcttccagca
     7441 tcagcagttt ttcccctgcc agatcggcca tcggtacgca ttcgcggttc gcccacgggt
     7501 gatcttcata gatagccagc aacattggct catcaaacaa cggcacttca atgaatgctt
     7561 cgctctcttt caccagcgcg aggatcacgc aatcgagttt gccgctgtcc agttgcgcca
     7621 gtaactggtg ggtctgtgct tcatgcagat acatttccag ctttggaaat gtctggtgca
     7681 gcatcgggat aatatgcggc agcaggtacg gtccaaccgt aggaatcaaa ccaatatgca
     7741 gcggtccgga catcgtctcg ccctgctggc ttgccatctc tttaaggact ttcacctcac
     7801 gcagcacggt acgcgcctga tccaccagca gcattcccgc ctgggtgaac aacactttac
     7861 ggctggtccg ctccagcaac atcacgccca gctcatcttc cagcttacga atttggccgc
     7921 taagcgtcgg ctggctaacg tggcaggaat ctgccgcacg ccgaaaatgg cggtgttcag
     7981 ccaatgccac caggtactca agatcacgaa tattcattat ccatcctcca tcgccacgat
     8041 agttcatggc gataggtaga atagcaatga acgattatcc ctatcaagca ttctgactga
     8101 taattgctca cagaaacgga gcggcacctc ttttaaccct tgaagtcact gcccgtttcg
     8161 agagtttctc aactcgaata actaaagcca acgtgaactt ttgcggatct ccaggatccg
     8221 cttttttttg ccataaaaaa gcccggcgat aagccaggct caaatttgta catataaatg
     8281 ttcttacccc aaccgcgcct gcgcaaaagc aatcgcttgg gccacctgct gcggtgagac
     8341 gccgcctttt gccgcacgct tgtcgaggca cgattgtagc gacagaatcg gatagacatc
     8401 ttcgccaatc acgtggctga atttctgcaa ctcgtcgagc ggcagatctt ccagcggttt
     8461 gccctgacga atggcttcca ccaccgcttc acccacgata tggtgcgcct cgcggaaagg
     8521 tacgcctttc gccaccaggt aatccgccag ttcggtggcg ttggcgtaac cctgctgagc
     8581 cgcttcctgg caacgtggac gtttcacctg aatgccgtcc agcaccagcg ccgccatatg
     8641 caggcagtcc agccaggtat cgaacgcgtc gaacagacct tctttgtctt cctgcatatc
     8701 tttgttgtaa gccagcggca aacctttcag cgtcatcatc atgccggtta acgcaccctg
     8761 cacccgaccg catttaccgc gaatcagctc cagcgcatcc gggtttttct tctgcggcat
     8821 taatgatgaa ccggaagtca cgcggtcaga aagctccaca aaccccgctt cgccggtgtt
     8881 aaagaaaatc agatcttcag caagacgcga cagatgcacc atgccgatag cggcagccga
     8941 cagcagttcc aacacatggt cacggtcaga aacgctgtcc agactgttac gggtcgccga
     9001 agcaaagccc agccagcctg ctaactgttc acggtcgatt tcataggccg ttcccgccag
     9061 cgcgccacag cctaatgggc tgacatccag acgcttaagc gcatcctgca aacggctttc
     9121 atcacgcgcc agcatctcaa cataggccag gcaccagtgc gcgaacgtca ccggctgggc
     9181 gcgttgcagg tgagtgtaac ctggcattac cgcgtcctga ttgttttgtg cggtttccac
     9241 cagcgccgat tgcagctgcc ggttagccgt cagtaactcg ctaacggtat ctttgcacca
     9301 cagtttcagg tcagtcgcta cctgatcatt acggctacgc ccggtatgca gctttttgcc
     9361 taactggccc actttgtcga tcagtttgcc ttccacccag ctatggatat cttcggcgtc
     9421 gctttcaagg atttgttgtg gcctggcgcg aacatcttcc agcaacacgt tcagcgcctc
     9481 ttccagttgc gcctgctctt ctgcggttaa cacgccgacc gttaccaggg ctttggacca
     9541 ggccacagag ccaacaatat cctgctccgc cagacggtaa tcaaagcgca gtgagtcgtt
     9601 gaattgtttg aaccgttgat ctgctgcctg ggtaaaacgc ccgccccaaa gtgccataac
     9661 tctgtttcct tattttgaaa ttcaatgccg gaaagctgcg cctccggcta acaaaactta
     9721 agctaaaatc cgcgtaccca tcggcatacc gttaaacagt gccggaagct gctccgcatg
     9781 acgccaggag gcgatatcta ccggacggcc cagcgtgcgg gccgcatcca gcgccgcgtt
     9841 cactttcact atcatgccgt cagtaataat gccctgctca atcagttgtt ctgctttcgc
     9901 ggcggtcatt tcggcaatgc gttgcccttt gccgtcgaga atgccgctga cgtcggagag
     9961 caaaatcaga tccgcgccca gcgttgccgc cagcgccgtt gccgcctggt cggcattgac
    10021 gttcatcagt tgcccttcgt ctgttacgcc aatggagctg accaccggca gataaccgtt
    10081 ctccagcaag gagttgataa gcttaggcga acctggctgc gccagtccaa catgacctaa
    10141 ctcttcatca agctgggtca ctttgacgct gtcgccatca ccgagaaaca aacctacagc
    10201 cgcaatctga tgtttcttcg cccatgccaa cagggtttta tttgccgttc ccgccagtgc
    10261 tccggtgata atgtctatct gatcagcagg cgtcacccgc aggccgtttt tctttttcac
    10321 cggcagattc agccctttca tcagctcatc caccacgcaa ccgccgccgt gcacaatcac
    10381 cagcggacgc tgatgtgact cacgataatt caccagtgcg ctaaacagac gttccagcgc
    10441 ctcttcacta tccagcagta cgccgcccag tttgataatt aatggattca tcattgcacc
    10501 cttaaataag agactgcgtt tccgcatagc cgaaacgaat attggcgcac tgtaccgctt
    10561 gtgccgccgc gcctttcagt aagttgtctt cggtcgccac aataatcaga tgctcgccct
    10621 gaacggcaaa cccgatatcg caaaatggca gcccaacgac atttttcagc gccggaacgc
    10681 ctttgtcata cagccgcacc agcggtttat gggcatacgc ctgctgtaac acttgcgcga
    10741 cttgcgcctg ggtcacaccc gatttcaggc ggcaggtaat ggtttcgaga atgccgcgcg
    10801 ggaaattgcc cagatgtggg gtgaagataa cgtcagcacc gaggtgtgtg gcgatctctg
    10861 gttgatggcg atgagtaaag acgccatacg gttgcaggct aacttcacaa aaactatttg
    10921 aaatcgctgc cttacgtcct gcaccgctca cgccgctggt ggcgttaatc gccggccact
    10981 gattgaggtc aagaagatcg gcatcaatca acggtttcag cgccagctgt gccgccgtcg
    11041 gataacagcc tggcaccgca atcaaattcg cttcttttaa tttattaccg caccactccg
    11101 ccagaccgta ggctgcctgt tccagcagtt ccgggtattg atgggtaaag ccgtaatatt
    11161 tttcatagaa ggtggcgtcg ttaacacgaa acgcgccgga aaggtcgaac accacgcagc
    11221 ccgcttcaag aaactgcggc gctaaatcgt ggctaacttc atgggcggtg gcgagaaaca
    11281 ctacgtccac ccctgggcta aactcgctga tatccgacat cggctgcaac ggcagctcaa
    11341 cgatgccttt tagctgcgga tgcaaatcgg agattaactt tcccgcatca ttgctttgcg
    11401 ctgaaacagt caaagcggtt atgttcatat gcggatggcg atttacatag gtcactagct
    11461 ctgcgccagc gtagccgctg gcacccacaa tcagcgtatt caacatcggg gctattcacc
    11521 ttcttatgtc tggttgccag gttaaacgta aaacattcac cttacggctg gtgggtttta
    11581 ttacgctcaa cgttagtgta tttttattca taaatactgc atgaatattg atactatcat
    11641 gaccagaggt gtgtcaacaa tgaaaaacaa attaccgcca tttatcgaga tttaccgcgc
    11701 tctgattgcc acaccttcaa taagcgctac ggaagaggca ctcgatcaaa gcaatgcaga
    11761 tttaatcact ctgctggcgg actggtttaa agatttgggc ttcaatgtgg aagtgcagcc
    11821 tgttccagga actcgcaaca aattcaatat gctggcaagt atcggacagg gggctggcgg
    11881 cttgttgctg gcggggcata ccgatacggt gccatttgat gacggtcgct ggacgcgcga
    11941 tccgtttaca ctgacggagc atgacggcaa gctttacggc ttaggcaccg ccgacatgaa
    12001 aggctttttt gcgtttatcc ttgatgcgct acgcgatatc gacgtcacga aactgaaaaa
    12061 accgctctac attctggcga ctgctgatga agaaaccagt atggccggag cgcgttattt
    12121 tgccgaaact accgccctgc gcccggattg cgccatcatt ggcgaaccga cgtcactaca
    12181 accggtacgc gcacataaag gtcatatctc taacgccatc cgtattcagg gtcagtcggg
    12241 gcactccagc gatccagcac gcggagttaa cgctatcgaa ctaatgcacg acgccatcgg
    12301 gcatattttg caattgcgcg ataacctgaa agaacgttat cactacgaag catttaccgt
    12361 gccataccct acgctcaacc tcgggcatat tcacggtggc gacgcttcta accgtatttg
    12421 cgcttgctgt gagttgcata tggatattcg tccgctgcct ggcatgacac tcaatgaact
    12481 taatggtttg ctcaacgatg cattggctcc ggtgatcgaa cgctggccgg gtcgtctgac
    12541 ggtcgacgag ctgcatccgc cgatccctgg ctatgaatgc ccaccgaatc atcaactggt
    12601 tgaagtggtt gagaaattgc tcggagcaaa aaccgaagtg gtgaattact gtaccgaagc
    12661 gccgtttatt caaacgttat gcccgacgct ggtgctcggt ccgggttcca tcaatcaagc
    12721 tcatcagcct gatgaatatc tggagacgcg gtttattaaa cccacccgtg agttgattat
    12781 ccaggtaatt caccattttt gctggcatta aaacgtaggc cggataaggc gctcgcgccg
    12841 catccggcac cgttgccaaa ctccggtgcc gcaataatgt cggatgcgat gcttgcgcat
    12901 cttatccgac ctacaccttt ggtgttatgt atggcgattt tttaacattt ccataagtta
    12961 cgcttattta aagcgtcgtg aatttaatgg cgtaaattcc tgctatttat tcgtttgctg
    13021 aagcgatttc gcagcatttg acgtcaccgc ttttacgtgg ctttataaaa gacgacgaaa
    13081 agcaaagccc gagcatattc gcgccaatgc gacgtgaagg atacagggct atcaaacgat
    13141 aagatggggt gtctggggta atatgaacga acaatattcc gcattgcgta gtaatgtcag
    13201 tatgctcggc aaagtgctgg gagaaaccat caaggatgcg ttgggagaac acattcttga
    13261 acgcgtagaa actatccgta agttgtccaa atcttcacgc gctggcaatg atgctaaccg
    13321 ccaggagtta ctcaccacct tacaaaattt gtcgaacgac gagctgctgc ccgttgcgcg
    13381 tgcgtttagt cagttcctga acctggccaa caccgccgag caataccaca gcatttcgcc
    13441 gaaaggcgaa gctgccagca acccggaagt gatcgcccgc accctgcgta aactgaaaaa
    13501 ccagccggaa ctgagcgaag acaccatcaa aaaagcagtg gaatcgctgt cgctggaact
    13561 ggtcctcacg gctcacccta ccgaaattac ccgtcgtaca ctgatccaca aaatggtgga
    13621 agtgaacgcc tgcttaaaac agctcgataa caaagatatc gctgactacg aacacaacca
    13681 gctgatgcgt cgcctgcgcc agttaatcgc ccagtcatgg cataccgatg aaatccgtaa
    13741 gctgcgtcca agcccggtag atgaagccaa atggggcttt gccgtagtgg aaaacagcct
    13801 gtggcaaggc gtaccaaatt acctgcgcga actgaacgaa caactggaag agaacctcgg
    13861 ctacaaactg cccgtcgaat ttgttccggt ccgttttact tcgtggatgg gcggcgaccg
    13921 cgacggcaac ccgaacgtca ctgccgatat cacccgccac gtcctgctac tcagccgctg
    13981 gaaagccacc gatttgttcc tgaaagatat tcaggtgctg gtttctgaac tgtcgatggt
    14041 tgaagcgccc cctgaactgc tggcgctggt tggcgaagaa ggtgccgcag aaccgtatcg
    14101 ctatctgatg aaaaacctgc gttctcgcct gatggcgaca caggcatggc tggaagcgcg
    14161 cctgaaaggc gaagaactgc caaaaccaga aggcctgctg acacaaaacg aagaactgtg
    14221 ggaaccgctc tacgcttgct accagtcact tcaggcgtgt ggcatgggta ttatcgccaa
    14281 cggcgatctg ctcgacaccc tgcgccgcgt gaaatgtttc ggcgtaccgc tggtccgtat
    14341 tgatatccgt caggagagca cccgccatac cgaagcgctg ggcgagctga cccgctacct
    14401 cggtatcggc gactacaaaa gctggtcaga ggccgacaaa caggcgttcc tgatccgcga
    14461 actgaactcc aaacgtccgc ttctgccgcg caactggcaa ccaagcgccg aaacgcgcga
    14521 agtgctcgat acctgccagg tgattgccga agcgccgcaa ggctccattg ccgcctacgt
    14581 gatctcgatg gcgaaaacgc cgtccgacgt actggctgtc cacctgctgc tgaaagaagc
    14641 gggtatcggg tttgcgatgc cggttgctcc gctgtttgaa accctcgatg acctgaacaa
    14701 cgccaacgat gtcatgaccc agctgctcaa tatcgactgg tatcgcggcc tgattcaggg
    14761 caaacagatg gtgatgattg gctattccga ctcagcaaaa gatgcgggcg tgatggcagc
    14821 gtcctgggcg caatatcagg cacaggatgc attaatcaaa acctgcgaaa aagcgggtat
    14881 tgagctgacg ttgttccacg gtcgcggcgg ttccattggt cgcggcggtg cacctgccca
    14941 tgcggcactg ctgtcacaac cgccaggaag cctgaaaggc ggcctgcgcg taaccgaaca
    15001 gggcgagatg atccgcttta aatatggtct gccagaaatc accgtcagca gcctgtcgct
    15061 ttataccggg gcgattctgg aagccaacct gctgccaccg ccggagccga aagagagctg
    15121 gcgtcgcatt atggatgaac tgtcagtcat ctcctgcgat gtctaccgcg gctacgtacg
    15181 tgaaaacaaa gattttgtgc cttacttgcg ctccgctacg ccggaacaag aactgggcaa
    15241 actgccgttg ggttcacgtc cggcgaaacg tcgcccaacc ggcggcgtcg agtcactacg
    15301 cgccattccg tggatcttcg cctggacgca aaaccgtctg atgctccccg cctggctggg
    15361 tgcaggtacg gcgctgcaaa aagtggtcga agacggcaaa cagagcgagc tggaggctat
    15421 gtgccgcgat tggccattct tctcgacgcg tctcggcatg ctggagatgg tcttcgccaa
    15481 agcagacctg tggctggcgg aatactatga ccaacgcctg gtagacaaag cactgtggcc
    15541 gttaggtaaa gagttacgca atctgcaaga agaagacatc aaagtggtgc tggcgattgc
    15601 caacgattcc catctgatgg ccgatctgcc gtggattgca gagtctattc agctacggaa
    15661 tatttacacc gacccgctga acgtattgca ggccgagttg ctgcaccgct cccgccaggc
    15721 agaaaaagaa ggccaggaac cggatcctcg cgtcgaacaa gcgttaatgg tcactattgc
    15781 cgggattgcg gcaggtatgc gtaataccgg ctaatcttcc tcttctgcaa accctcgtgc
    15841 ttttgcgcga gggttttctg aaatacttct gttctaacac cctcgttttc aatatatttc
    15901 tgtctgcatt ttattcaaat tctgaatata ccttcagata tccttaagga attgtcgtta
    15961 cattcggcga tatttttttc aagacaggtt cttactatgc attccacaga agtccaggct
    16021 aaaccccttt ttagctggaa agccctgggt tgggcactgc tctacttttg gtttttctct
    16081 actctgctac aggccattat ttacatcagt ggttatagtg gcaccaacgg cattcgcgac
    16141 tcgctgttat tcagctcgct gtggttgatc ccggtattcc tctttccgaa gcggattaaa
    16201 attattgccg cagtaatcgg cgtggtgcta tgggcggcct ctctggcggc gctatgctac
    16261 tacgtcatct acgggcagga gttctcgcag agcgttctgt ttgtgatgtt cgaaaccaac
    16321 accaacgaag ccagcgagta tttaagccag tatttcagcc tgaaaattgt gcttatcgcg
    16381 ctggcctata cggcggtggc agttctgctg tggacacgcc tgcacccggt ctatattcca
    16441 aagccgtggc gttatgttgt ctcttttgcc ctgctttatg gcttgattct gcatccgatc
    16501 gccatgaata cgtttatcaa aaacaagccg tttgagaaaa cgttggataa cctggcctcg
    16561 cgtatggagc ctgccgcacc gtggcaattc ctgaccggct attatcagta tcgtcagcaa
    16621 ctaaactcgc taacaaagtt actgaatgaa aataatgcct tgccgccact ggctaatttc
    16681 aaagatgaat cgggtaacga accgcgcact ttagtgctgg tgattggcga gtcgacccag
    16741 cgcggacgca tgagtctgta cggttatccg cgtgaaacca cgccggagct ggatgcgctg
    16801 cataaaaccg atccgaatct gaccgtgttt aataacgtag ttacgtctcg tccgtacacc
    16861 attgaaatcc tgcaacaggc gctgaccttt gccaatgaaa agaacccgga tctgtatctg
    16921 acgcagccgt cgctgatgaa catgatgaaa caggcgggtt ataaaacctt ctggatcacc
    16981 aaccagcaga cgatgaccgc ccgcaatacc atgctgacgg tattttcgcg ccagaccgac
    17041 aagcagtact acatgaacca gcaacgtacg cagagtgcgc gtgaatacga caccaacgtg
    17101 ctgaagccgt tccaggatgt gctgaatgac cctgcgccga agaaactgat catcgttcat
    17161 ctgctgggta cgcatatcaa atacaaatac cgctacccgg aaaatcaggg caagtttgat
    17221 ggcaataccg atcatgttcc gccaggatta agcgcagaag agctggaatc atataacgat
    17281 tatgacaacg ctaacttgta taacgatcat gtggttgcca gcctgattaa agactttaaa
    17341 gcggcagacc cgaacggatt cctggtttac ttctctgacc acggtgaaga ggtttacgac
    17401 acgccgccgc acaaaactca ggggcgtaac gaagacaacc cgacgcgcca catgtacacc
    17461 attccgttcc tgctgtggac gtcggaaaaa tggcaagcga ctcatccccg tgatttctca
    17521 caggatgtcg atcgtaaata cagcctggcg gaactgatcc acacctggtc agatttggcg
    17581 ggcttatctt acgacggtta cgatccaacc cgttcagtgg tgaatccgca gttcaaagaa
    17641 actacccgct ggattggtaa cccgtacaag aaaaacgcgc tgatcgatta cgacactctg
    17701 ccgtatggcg accaggtagg taatcagtaa ttatcagcca ggcgctattt gccggatgcg
    17761 acgcttgacg cgtcttatcc ggcctacaga tcgcggcggt aggctggata aggcgtttac
    17821 gccacatccg gcaatacaac tgccgcaatg tttacatcga actcccctca ggccacactt
    17881 ttcaggtggc cttttttaat aatgccagtt actgttttcc cgtctacata cctaagggaa
    17941 taacatgtat cacgacatca gctatctgct ctcccgcctg atcaacggcc cgctgtccct
    18001 gcgccagatt tactttgcca gcagtaacgg cccggtgccc gatcttgcgt atcaggtcga
    18061 ttttccacgg ctggaaattg tgctggaagg tgagtttgtt gataccggcg ctggagcaac
    18121 gttagttccc ggcgatgtgc tgtacgttgc cgctggtggc tggaatttcc cacaatggaa
    18181 aacacccgct accaccttta gcgtgctgtt tggcaaacag caactcggct tcagcgtcgt
    18241 gcaatgggat ggcaaacaat atcaaaatct ggcgaagcaa cacgtcgccc gacgcggccc
    18301 acgtataggt tcttttctgc tacaaacgct caatgaaatg caaatgcagc cgcaggagca
    18361 gcaaacggca aggcttatcg tagccagcct gcttagccac tgccgcgatt tgctcggcag
    18421 ccaaatccag accgcctcac gcagccaggc actatttgaa gctattcgcg attatatcga
    18481 cgaacgctat gcctccgcgc ttacccgcga atctgtcgca caggcattct atatttcacc
    18541 aaactatctc tcgcacctgt ttcaaaaaac gggggccatt ggttttaacg aatacctgaa
    18601 tcacacgcga ctggagcacg ctaagacgtt attgaaaggc tatgacctga aggtaaaaga
    18661 agtggcgcac gcctgcggct ttgtcgacag caactacttc tgccgtctgt ttcgtaaaaa
    18721 caccgaacgc tcgccgtcgg aatatcgccg acagtaccac agccaactga cagaaaaacc
    18781 gactactcca gaataagatg ggtttgctgc ggcgcagaaa gcactttgcg caccgcgctc
    18841 attacccgct gcggctcacg caggaatgcg tagatgctgc attgcacata gcggcaatgt
    18901 tcaaatcgct cggcaccggc aagctcgata tccgtaatca acagagcaac atccgcccga
    18961 cggatatcct cgtccgctaa acgattctcc gttcccagcg cgccctgagt ttcaatgctg
    19021 acgccccact tctctaactg gcacaacttt tccagccgtt ccgccgccat ataagtatgc
    19081 gccacgccgc ttacgcaggc ggttactgcc accaggtatg ccattttaac ctcccacggt
    19141 aacctgaaat ccggcccgtt ctgccatttc gcgcatcgtt gccacatcgg ctgatgacgg
    19201 cgcagcaaca tccttcatcg accatgtttt ccccagcagg cggtatttcg gctcgccgta
    19261 ctgatgaaac ggcaacagat ggatctgcct gatattcagc gggatcagca cgtccagcgc
    19321 ctgctgcata ttctcccggc tgagtgtgaa accggggatc agcggcaaac gcgggatcac
    19381 gttgacaccc tcactcacca gcaaacgcag gttctccagt acgcgtggca ggttcatctt
    19441 caccacatcc cgcgcctgag tcgcgtccat aatttttaaa tcgaacaaca cttcatcgca
    19501 caatttcgcc agcggtaaca gcttgctggc tggtgcgtct ccggcagttt caatggcaca
    19561 tgagacaccc cacagccgca gtcgctgtaa aaaacgggta gcaaactccg cctgcattaa
    19621 cacttcgccg ccagaaagcg tgacgccgcc gccggacgtg cgaaaaaaaa tgtcatcttt
    19681 catcacttcc cgttccagag cgtcaaggct gatatcgcga ccaatccgtt caaacgcccc
    19741 ggaggggcat tcatccgcat cacgcaaaca tttcgcacag tgcagacatt tcgcctctct
    19801 gcgtaccgtc tggattttgc cggagatcga ctccggatta gcacaccacg ggcaaagatg
    19861 cggacagcct ttaaaaaaga ccaccgtacg aatgccctcg ccgtcattca gtgaataacg
    19921 ctgaatgttg aaaatgcgcg ccacatcatt gaggcgcgtt tccacaacgt tacagctgat
    19981 gcgctgtccg gcggatgatg tcatcctgga tctccttcga cagttcgaca aagaaggcgc
    20041 tgtatccggc aacgcgcacc accagcccgg cataatcttg tgggcgctgt tgcgcttccc
    20101 acaacgtgtc ggcgttcacc acgttaaact gaatatgctg caacttaagc tgggtaaacg
    20161 cccgtaagaa gtcggccagt ttgcgtaatc ccgcttcacc ttccagggtc gccggagtga
    20221 atttcacgtt cagcaacgta ccgttagaca gcagcgtatt atcgagctta ctgactgact
    20281 tcagtaccgc tgttggccct tgttcgtcct ggcccagcat aggtgacaag ccgccgtctg
    20341 ccagctgttc tccggcaaaa cgaccgtctg gcgtcgcgcc aaccaccgat cccaacggga
    20401 cgtgagcaga aacggtatac gatcccggcg tgaagtaacc accgcgcggg ttctggtatt
    20461 tttccacttc tttgcagtag tggcgcaaca gttcggcgct aatgttatcc acctcgtcga
    20521 tatcgttacc atatttctca aagcggttaa ttaagcgagc gcggactttt tcgccttctg
    20581 gcgttgcaaa gttggctttt aataccgaca gcaattcgtc aaaacttaaa cgctgttgct
    20641 caaaaaccat ccctttcaac gcatggagag aatcgctcag gttggcgata ccgatcccct
    20701 gtacgccgga gaagttataa cgcgcgccgc catcggtaat atcgcgacct ttttccagac
    20761 aatcgctgat aaaagacgag agcagcggta caggtgccca gtcgcgatgg ccgatatcac
    20821 aaatattgct gccctcaacc atcagggtga tgtagtggct gatcttggcg cggatctgct
    20881 ccagtaaacc ttcataagtc agcgcagcat tgccttcatt ttcatgcagg cagatttcca
    20941 tcactttcag cagattaaac atcgcgatgt catgcaagcc gtaggttctg ccgggaatag
    21001 ataattccac acagcccact acggaatagt cgcgcgcatc ttccagcgaa acgccacggt
    21061 tgaggaacgc tggtaccacc acttcatcgt taaagatttg cggaataccg gtgccgaggc
    21121 gaatggtttc ggcggttttc atcaggaatg gcgtgtcgat caaggcgtta gtgcgcacgc
    21181 cgaggttcgg ttgcggtaat tgcacgcttt gataggcgtc aaggcaaagg aacgaaagca
    21241 cgttcaccgc gctacgtccg ttctcggtta acccgccgag cagtgcggta tagccggtcg
    21301 ggaaacctgc gaaataacgc gcgctgctgg tggagcgcaa cagcacgatg tcgttgcatt
    21361 tcacccataa agattcgagc agttctttca ggaacgccgg atcttcgccc ggggttaaag
    21421 atgcctgata gaacggcaac atatactggt cgaagcgccc taaagacagc gaactggcgt
    21481 tggattcgta ttgcagaatg atgttcatgt accagaataa ctggcacgcc tgccagaacg
    21541 tctgcggctt atgttcggcg ttatggcgag agatctccgc aatagtcagc agttcttcgc
    21601 gacgctgggc atctgtgcag tttgccgcca tcgtttccgc cagtacagcg taacgcaaaa
    21661 tgtgtttctg cgaggcttcc agcagtaaca gcgctgcctg ataaaagtga ttctccggct
    21721 gttgctgaca atgttgctgc atctgtgcca ccaactcccc cagcccgtga ttcaacaggc
    21781 gtgggtaatc aataataatg tgcccctggc ctttatccgt ctggttaatg ctgaaaatct
    21841 gcgtgctggt cgcggctttt acttcatccg tcatctgccc gttgatgaaa tctttcatcg
    21901 aacgtttttc ccagtacggg aacaactctt cacgatagat acgtttatct tcttcgctga
    21961 tagcaaagcg gtcctgcgga cgcgtcggga attgatccag ctctttcagc agccagtaag
    22021 ggtccatttc cggcgacata atcccggcgc gcggttttac ggtgcggtta ccggcaatca
    22081 gttcttcatc acgaatcgaa atttcaacat gttcaaggat atacgctgtt gctttcgccc
    22141 ggcgcaatat caccggttcg ccttcggttt gccgatggct ggcggtataa agcagcgccc
    22201 gctccagcga gatttcacgg gtattggcaa acagtgcagt tttgaggcga gagatacgat
    22261 tcgtcatgag gaactcctgt gggaaagcga acccgccctt gcgagcggga gaagaactta
    22321 agcggtttgc gccaggtgag cttcaatttt gctcataatg gcgtcagcac gttttactgc
    22381 atcgctgatg ttgacgcgca cgatggtctt gccagcaaaa cgctcttcaa acttgatgcc
    22441 gatatctttg gtcagaatca ccatatcagc gctcgccaca tcttccgcag tcagttcatt
    22501 ttccagacca attgacccct gggtttctac tttcacttcc cagcctttcg ctttcgcggc
    22561 actttccagc gcctctgctg ccatataggt atgggcaaca cctgaaggac atgcggttac
    22621 tgcaataatt ttcgtcatgg ctcgttcctc aattaattaa tttcaaaatc caaatccagg
    22681 tcgtcttctt tttcatcagt cgaagaccca tttttacgcg ccagactttt cagcacgttc
    22741 acacaaacag ccgtcaccac tgctccgaca gccactgctg cgatataacc cagcttgcct
    22801 tcaaccaccg gcagcacaat cagaccaccc cagcctgcat agcactgcgc accgaccagc
    22861 gccgcagtta ctgcaccaca aactgaaccg accatgatgg aaggaatgac acgcagcgga
    22921 tcggcagcgg cgaaaggaat cgccccttca gtaacgccaa cacagcccat caccagtgcc
    22981 gctttgccgg tttcgcgctc ttctgcggag aaatttttac gaccaatcag cgtcgccagc
    23041 cccatcccca gcggcgggat acaaataccc acggcagcga tgaccaccac ggtataaaca
    23101 ccctgagcaa cgcaaatcag catgaaggca taggctactt tgttaaccgg accgcccata
    23161 tcgaacgcca gcatcagacc catgatcacc gccagcataa caatgctgcc ctgctgcatc
    23221 ccctgaagcc actgagtcag gctgttggtc aacgccccta caggctcgcc caagccccac
    23281 atcatgatgc ctgcggtaat cagtgtgccg acgataggaa taatgaagat aggcatcacc
    23341 gagcgcagaa ctttatgcac cggaattttc ttcaggtaat gcaccacgat gccgccgata
    23401 atcccggcga tcagtgcacc aaagaagccc gcaccaaagc tgttaccaac ccaggcaccg
    23461 atagcgcacg gagccagcgc agaacgctct gcaatggagt aaccaatgta agcggcgagg
    23521 aaaggcacca tcagcgtcaa acctgcaacg ccgatatcaa acagtttttt caaattcgga
    23581 tcggctacgg catccggcac tgcgcctttg ccatacaaca tgacggaaac cgccagcaaa
    23641 ataccgcccg ataccacgaa gggaatcatg tgtgaaacgc ccgtcattaa atgctgacgg
    23701 gtatttttca ggatctgcac caactcattc ataagctgct cccgggcttg cgaaagccca
    23761 tgtccatatt tttgatggag gaaacaatag gcaatcgcag gcggagcaaa cagtggataa
    23821 aaaggccatt tactggactt ttgtaatatc agtacaaaaa tgcgatccgc ctcataactt
    23881 gcgataaacc ggaagcatgc cgcaaaacgg cgtcttgtga gacgcgtcgc aaattgacgg
    23941 ttacattaca tttgtccagc ttttggcaga tttgtcacat ggcaggccgt tgccgtgcgc
    24001 tcttatcctg taaacagaaa accgtaccgg gagagaggca atggccctga ttgtggaatt
    24061 tatttgtgag ctacctaacg gcgtacatgc gcgtccggca agccacgttg aaacgctgtg
    24121 taatactttt tcatcacaaa ttgagtggca taacctgcgc actgaccgca agggcaacgc
    24181 caaaagcgcc cttgcgctga ttggcaccga tacgctggcg ggcgataact gccagttact
    24241 gatttccggg gccgacgaac aggaagcgca ccagcgttta agccaatggc tgcgcgatga
    24301 attcccccac tgcgatgcgc cgctggcgga agttaaatct gacgaactgg aaccactgcc
    24361 ggtttcactg accaatctga atccgcaaat tatccgcgcc cgcaccgtgt gcagcggtag
    24421 tgcaggcggc attctgacgc cgatctcttc tttagatctc aatgcgctga gtaatcttcc
    24481 cgcagccaaa gacgttgacg ccgagcaatc cgcactggaa aatggcctga cgctggtact
    24541 gaaaaacatt gagtttcgtc tgctggatag cgacggtgct accagctcga tactggaagc
    24601 tcaccgatcc ctggctggcg atacatcttt acgtcagcac ttactgacag gcgtcagcga
    24661 gggattaagc tgtgccgaag cgattgtcgc cagtgcgaat cacttttgcg aagagttcgc
    24721 ccgttccagc agcagctacc tgcaagaacg tgccctggac gtacgcgacg tctgcttcca
    24781 gttactccag caaatctacg gtgagcaacg cttcccggca ccgggcaaac tgacgcagcc
    24841 cgccatttgt atggctgatg aactgactcc cagccagttc ctcgaactgg ataaaaatca
    24901 cctcaaaggt ctgttactga aaagtggcgg caccacctca cataccgtaa ttcttgcccg
    24961 ttcgttcaac attccgacac tggttggtgt agatattgat gcccttactc cgtggcagca
    25021 tcaaacgatt tatatcgacg gcaacgccgg ggcgattgtg gttgagccag gggaagccgt
    25081 ggcccgttat tatcagcaag aagcccgcgt acaggacgcc cttcgtgagc aacagcgtgt
    25141 ctggctgacc caacaagccc gtaccgctga cggtatccgc attgaaattg ccgctaacat
    25201 cgctcactcc gtggaagcgc aggccgcatt cggcaatggt gcggaaggcg ttggtttgtt
    25261 ccgcactgaa atgctctata tggatcgcac cagtgcaccg ggcgaaagcg agctgtacaa
    25321 cattttttgt caggcgctgg agtctgccaa cggacgcagc attattgtgc gcactatgga
    25381 cattggcggc gacaaacccg ttgattatct gaacattccg gcagaagcta acccgttcct
    25441 cggttatcgc gctgtgcgta tttatgaaga atacgcctcg ctgttcacca cacaactacg
    25501 gtcgatcctg cgcgcctccg ctcacggcag cctgaaaatc atgatcccga tgatctcctc
    25561 aatggaagag atcttatggg tgaaagaaaa actggcggaa gccaaacagc aactgcgtaa
    25621 cgaacacatt ccgtttgatg agaagatcca gcttggcatt atgctggaag taccgtcggt
    25681 gatgttcatc atcgatcaat gctgcgaaga gattgatttc tttagtattg gtagtaatga
    25741 cctgacgcaa tatctgctgg cagtggatcg cgataacgct aaggttactc gtcactacaa
    25801 cagcctgaat ccggcattct tgcgggcgct cgattacgcc gtgcaggcgg tgcatcgcca
    25861 gggcaaatgg attggtctgt gcggtgagct gggagcgaaa ggttccgtgc tgccgttgct
    25921 ggtcggctta gggctggatg aactcagcat gagcgcacca tcaattccgg cggcgaaagc
    25981 gcggatgacg caacttgata gccgtgagtg ccgcaagttg ctcaaccagg caatggcctg
    26041 ccgtacttcg ctggaagtgg aacacctgct ggcgcaattc cgcatgaccc aacaggacgc
    26101 accgctggtc accgccgagt gcatcacgct ggaaagtgac tggcgcagca aagaagaagt
    26161 gctcaaaggc atgaccgata acctgctgct ggcgggccgc tgccgctatc cgcgtaaact
    26221 ggaagccgac ttatgggcgc gcgaggccgt tttctctacc ggtctgggct ttagttttgc
    26281 cattccacac agcaaatcag aacacattga gcaatccacc atcagcgtgg cgcgtctgca
    26341 agcgccggtg cgctggggcg atgatgaagc gcaattcatc attatgttaa ccctgaacaa
    26401 acacgctgcg ggcgatcagc acatgcgcat tttctctcgc ctcgctcgcc gcatcatgca
    26461 cgaagaattc cgtaacgcgc tggttaacgc cgcctctgcc gacgctatcg ccagcctgct
    26521 gcaacatgaa ctggaactgt aaaaggaaac atcatggaac tgtatctgga caccgctaac
    26581 gtcgcagaag tcgaacgtct ggcacgcata ttccccattg ccggggtgac aactaacccg
    26641 agcattatcg ctgccagcaa ggagtccatc tgggaagttc tgccgcgtct gcaaaaagcg
    26701 attggtgatg agggcattct gtttgctcag actatgagtc gtgacgcgca gggtatggtg
    26761 gaagaagcga agcgcctgcg cgacgccatt ccgggcattg tggtgaaaat cccggtgact
    26821 tccgaaggtc tggccgccat taagatgctg aaaaaagagg gtattactac gcttggcacc
    26881 gcagtgtaca gtgctgcaca agggttactg gccgcgctgg caggggcaaa atacgttgct
    26941 ccgtatgtta accgcgtaga tgcccagggc ggggacggca ttcgtacggt tcaggagctg
    27001 caagcgctgt tagaaatgca cgcaccagaa agcatggtgc tggcggccag ctttaaaacg
    27061 ccgcgtcagg cgctggactg tttactggca ggatgtgaat ccatcaccct gcccttagat
    27121 gtagcgcaac aaatgctcaa cacccctgcg gtagagtcag ctatagagaa gttcgaacac
    27181 gactggaatg ccgcatttgg cactactcat ctctaaagga gcaattatgg accgcattat
    27241 tcaatcaccg ggtaaataca tccagggcgc tgatgtgatt aatcgtctgg gcgaatacct
    27301 gaagccgctg gcagaacgct ggttagtggt gggtgacaaa tttgttttag gttttgctca
    27361 atccactgtc gagaaaagct ttaaagatgc tggactggta gtagaaattg cgccgtttgg
    27421 cggtgaatgt tcgcaaaatg agatcgatcg tctgcgtggc atcgcggaga ctgcgcagtg
    27481 tggcgcaatt ctcggtatcg gtggcggaaa aaccctcgat actgccaaag cactggcaca
    27541 tttcatgggt gttccggtag cgatcgcacc gactatcgcc tctaccgatg caccgtgcag
    27601 cgcattgtct gttatctaca ccgatgaggg tgagtttgac cgctatctac tgttgccaaa
    27661 taacccgaat atggtcattg tcgacaccaa aatcgtcgct ggcgctcctg cacgtctgtt
    27721 agcggcgggt atcggcgatg cgctggcaac ctggtttgaa gcacgcgcct gttcccgtag
    27781 cggcgctacc actatggcgg gcggcaagtg cacccaggcg gcgctggcac tggcagaact
    27841 gtgctacaac actctgctgg aagaaggcga aaaagcgatg cttgccgccg aacagcatgt
    27901 agtaacccct gcgctggaac gcgtgattga agcgaacacc tatttaagcg gtgttggttt
    27961 tgaaagcggc ggactggcgg cggctcatgc ggtgcataac ggcctgaccg ctatcccgga
    28021 cgcgcatcac tattatcacg gtgaaaaagt ggcattcggt acgctgacgc agctggtact
    28081 ggaaaacgcg ccggttgagg aaatcgaaac cgtagctgca tttagccatg cggtaggttt
    28141 gccaataact ctcgctcaac tggatattaa agaagatgtc ccggcgaaaa tgcgaattgt
    28201 ggcagaagcg gcatgtgcag aaggtgaaac catccacaac atgcctggcg gcgcgacgcc
    28261 agatcaggtt tacgccactc tgctggtagc cgaccagtac ggtcagcgtt tcctgcaaga
    28321 gtgggaataa cctactccaa actcccggct tgtccgggag tttgaacgca aaattgcctg
    28381 atgcgctacg cttatcaggc ctacgcaatc tctgcaatat attgaatttg cgtgcttttt
    28441 aggccagata aggcgttcac gccgcatccg gcactctgtg ccagtaatct gaaactccta
    28501 cgttgttcag gagtttttta tccgcttctg gacattttct ctacagaaca atcgttagcc
    28561 cggaaggtcg aatctgtgac tcttattttc acggacgaac cagatgaaag cttcactggc
    28621 gctgctcagc ctgctaacgg catttaccag ccactcctta aaatctcccg ccgttccacc
    28681 aaccgtggta caaatccagg ccaataccaa ccttgccatc gcagacggtg ccagacagca
    28741 aatcggcaac acgctatttt acgatccggc gtatgtgcgg cttacctatc ctggcggtga
    28801 tgtcccgcaa gaacgcggtg tatgttccga tgtggtgatc cgtgcattgc gcagccagaa
    28861 ggtcgatttg cagaaactgg ttcacgaaga tatggcgaag aattttgccg agtacccgca
    28921 aaaatggaag ttaaagcgcc cggacagcaa catcgatcac cgtcgggtgc ctaatctgga
    28981 aacctggttt agccgccaca acaaaacacg ccccatcagt aaaaatgccg gtgactatca
    29041 agcgggcgat attgtctcct ggcgattaga taacgggctg gcgcatattg gggtggtgtc
    29101 agatggcttc gcccgcgacg gtacgccact ggtgatacat aacattggcg caggcgcgca
    29161 ggaagaagat gtgctgttta gctggcggat ggtggggcat tatcgttatt ttgttaaata
    29221 agcgatcgcc ccacatgaag aaaaaattca gatgcttttt acactgacag cttctttttt
    29281 cttacggctc accagcgcca gtgccagcac aatcagcaca ataccgctac cttcaacggc
    29341 ccccggattc tcaccgagca gccaccagga aaagagtacg ccgcaaacgg gaacggccag
    29401 cgtgcttaag ctggcaatac tggcaggcaa gtttttcaat acaaacaacc ataagctcca
    29461 cgccagcgcc gtcgccagaa tcgcactgta ggccagcgcc cagaacacgg tgggctgcca
    29521 gtcaatttca cgttgcggca ccagtaaagc gaccacactc atcaccagcg ccgcatagag
    29581 catctgccag gatgttaacg ataataaatc cacgcgcgga tgacgggtgt ataggcgttt
    29641 agcaacaatc gcgctcgccc cccaactaac accggagagg attgccagca tggcactttt
    29701 catcgaagag aaatcgagtt gccacggctg caacaccaaa aataagccga aagcggcaat
    29761 cagaatcgcg aaatattgcc cacgtcgcag gcgttcaccg agaaacaacg cggcgaaaat
    29821 caccacccag aacggcatgg tatagctcag gatcgccacc ttccccgcac ctccgctgac
    29881 caacgcccac tgcgccagac caaccatccc gcaggtttgt aacagggcaa tggctaaggt
    29941 gtatttaaac ggcgtcgggt gcattccgcg accacgtaat aaaaggacga tgaataaaac
    30001 gagagcgccg aaaatgcagc gtaaggcggt aaagtcgaag gcaccgatgt aactggtgac
    30061 ttgcttcatg aaaatccagc tataactcca gataagtgtg agcacaacca ggccactgat
    30121 tgccagtggg ttgctctttc ctgcggcaga catgagagat ccagtgtgta gttttcctgt
    30181 ggacactata cgccggtaaa gaaacttcag cgactgcctg aaagacagtc actgaagaag
    30241 gtcagattac agcaggtcga aacggtcgag gttcatcact ttcacccatg ccgctacgaa
    30301 gtctttaaca aacttctcgt gggcatcgct gctggcgtaa acttccgcta ccgcacgcag
    30361 gacagagtta gaaccaaata ccagatccgc acggctggct gtgtatttca cttcgccggt
    30421 ttcgcggtca cgtccttcga acagctcttt cgattcgtca gtcgctttcc actcgtaacg
    30481 catatccagc aagttcacga agaagtcatt gctcaatacg ccaacgcggt cagtgaagac
    30541 gccgtttttg ctgctatcga agttggcacc cagtacacgc atcccgccca ccagcgcagt
    30601 catttccggc gcggtcagcg tcagttgctg tgctttgtcg atcagcagtg actcggtggt
    30661 ggaaacgtcc agacgagcgc gatagttacg gaaaccgtca gcaattggct ccagcagctc
    30721 aaacatctca atgtcagtct gatcctgacg cgcatcaacg cgacccggcg caaacggtac
    30781 atgaatgctc aaacctgcgg cgcttgcggc tttctcaaca ccaaccacac cagccagcac
    30841 gatgatatcc gccagcgagg ctttaccaga ctctttctgg attttctcca gaacaggcag
    30901 agcacgaacg gctgcggcgt tcacatccca gtcgcgctgc ggcattaatg ccagacgcgc
    30961 accgttggca ccaccgcgtt tgtcgccacc acggaaggta gaagcagatg cccaggccac
    31021 cgataccagc tcactaacag acagaccaga atccgcaatc gcgaatttca gatcgataat
    31081 gtcctgctcg gtcgggttgt agatcggctg cggcagcgga tcttgccaga tcagatcttc
    31141 tttcggcact tccggcccga tgtagcgaga tttcggcccc atatccctgt gcgtcagttt
    31201 gaaccaggca cgggcaaagg cttcgttgaa cgcctgcgga tcgttgagga aacgacgaga
    31261 gatcttctcg aactcaggat caaaacgcag cgtcaggtcg gtcaccagca ttgtcggttt
    31321 acgtttcttc gacggatcga acggatccgg gataatttcc ggtgcgtcta ccgcttcgaa
    31381 ctggattgct ccagccggac tgcgggtctg tacccactca tatttgaaca ggttctcgaa
    31441 gaaatagttg ctccactggg tcggcgtctg ggtccagaca acttccagac cagaggtaat
    31501 ggcatctgcg ccaacgccgc tgccgtaagt gctcgcccag cctaaacctt gttcttcaat
    31561 cggtgcagct tctggatcag gacctacatt tgatgtcgga ccggcaccgt gagttttacc
    31621 cagcgtatga ccacccgcaa tcagcgccac ggtttcttcg tcgttcatgc ccatgttgcc
    31681 gaaggtcgca cggatagctg ctgccgcaga aagcggttcg ccgctgtgat ccgggccttc
    31741 cgggttaacg taaatcagac ccatctcggt tgcacccagc ggtgctttcg ccagcgcttc
    31801 cggatgacgg tgagtcagcc aggctttttc atcaccccag ttaacatcca gatccggttc
    31861 ccagacgtct tcacgaccgg caccaaaacc gaaggtacgg aagccggagt tttccagcgc
    31921 cacgttacct gcgaggataa acaggtcggc ccaggagatt ttctgaccat atttctgttt
    31981 gattggccac aacaggcgac gcgctttatc aaggcttacg ttatccggcc aggagttcag
    32041 cggtgcaaaa cgttgctgac cacgacccgc gccgccgcgt ccatcgattg aacggtaagt
    32101 acctgcgccg tgccaggcca tacgaataaa cagaccggcg tagctgcccc agtcggctgg
    32161 ccaccacggc tgagattctg tcagcagggc tttcagatct tttttcaggc cgtagtaatc
    32221 taatttgctg aattctttgc ggtagtcaaa gtcctcaccc agcgggttag aacgattaga
    32281 atgttggttt aacaggtcaa cacggagttg atttggccac cagtcgcgag tggttgtgcc
    32341 cgcccccgcg ctctggtcgt gaccgccctg atggaacggg catttgccag tggctgtcgt
    32401 gttatggata tcgtctgacg tgctcatcaa tgtgctcccc tctacagtgt taccgttacg
    32461 atacacagcg ttagagagaa gttataattg aattaatcca cggatgcgat agttgagatc
    32521 ttacatattg ttggttaaag agatgtaggt caaattgatc gtaattaagc ttttttgatt
    32581 atgtccgaat tcggacaaat gctttataaa aaaggggttt agcttcatat ccttcaagta
    32641 atgagggaga aaacaaggct gacaacctta ttttcccgcc ctcatttcga ggcagcattt
    32701 tgtgctctgt ttaaaatttg tgatcactgt gtgattttca caaaagccac actatttata
    32761 aaccaggtcg aacacccagc gtatggcaaa tcgcgtaact catttcagcg cggttaagcg
    32821 tatagaagtg gaaatctttc actccttcac ggcttaaaat cttcaccata tccatggcaa
    32881 tattcgcgcc caccagtttg cgggtttcgg catcatcatc caggccgtcg aacatttgcg
    32941 ccatccacgc cggaatacgc acgttggtca tatcggcaaa tttcttcgcc tgtttaaagt
    33001 tagataccgg caaaattccc ggaataattt ccacatcaat gcccgccgat acacagcggt
    33061 cacgaaaacg caggtagctt tcgacatcga agaagaactg agtaatcgcg cggttggctc
    33121 cggcatcgac tttgcgtttc agattaagca aatccgcctg agcgcttttt gcttccgggt
    33181 gaacttccgg atacgccgcc acggagatat cgaaatctgc cacttctttt aatagcgtca
    33241 ccaggtcaga agcatacatt tctggcttac cacttcccgg cggcagatcg ccacgcagcg
    33301 ccacgatatg acgaataccg ttattccagt agtcgcgtgc aatggtgcgc aactcgtcgg
    33361 gcgtcgcatc aatgcaagta agatgcggtg ccgcttccag accagtgcga tctttaatgc
    33421 ctttaataat gctgtgcgta cggtcgcgct cgccggagtt cgcgccatag gtcaccgata
    33481 caaacttcgg tttcaggctg ctaaggcgat cgatggagtt ccacagggtc tgctccattt
    33541 cactggtacg cggcgggaaa aactcgaacg aaacgttaat ctgcccctgg acttctgcca
    33601 ggcgctgatt cagggcatcc cgctggctgg cgtgaaaaaa gctcatacct tacctcatca
    33661 atcgcttgta gttatatgtt gtgttgtgca catctatccg tttagacgtc cagatgtaaa
    33721 gatgaaggaa aagccgaagg gcgtcaaccg aaaaatgacc aatgatggtg aggaaaaatc
    33781 agggaagatg aaaaaacttc aggatggtaa gaaaaagaaa atccggtatg caaagcaaac
    33841 cggactgcaa atataaacta accatgtact tattgcttct tgccggattc gtcgtaaacg
    33901 cttaatccgg cctaatccta tgataattca atatatttca ttagaaacca taaacccgaa
    33961 aacatgagta ccgggcatta ttaaatttct gaaattacaa caactgtgcc agccggttga
    34021 tatccgactg aatcgccccg gcggtgacgt cgcgccccgc gccaggccca cggatcacca
    34081 gcgggttgtc gcgataccag cggctttcga tggcaaagac gttatcgcac ggcagcagtg
    34141 atgccagcgg atggtcttca cgcaccgcct ccacgcccac acgcgctttg ccgttggcat
    34201 cgaaacgcgc cacgtagcgc agcaccagcc ccatttcgcg ggccgcttcc agccgttgca
    34261 ccatctgctc gttaagttca tcgccatttt caaagaaatg gtcgatgctg ccgccttcgc
    34321 aatgagcagg caccagcgat tccacacgta cctggtccgg ttcaatgttg taacccgctt
    34381 cacgcgccag aattaccagc ttgcgcatta cgtctttgcc ggagaggtcg tcacgcggat
    34441 caggctcggt taagccctgc tgccacgcct gatccaccag ctcggtaaac ggcacgctac
    34501 cgtcgaattg caggaacagc caggagagcg tgccggagaa gatcccgctg atcgacaaaa
    34561 tagtatcgcc gctgtcgatc agatcgcgca cggtgtggtt gatcggcaag cccgcaccga
    34621 cggtggcatt gtacagccag tgacgcccgg ttttttcgaa ggcgtcgtgg atctggcgat
    34681 atttattgct gtcgctggct cccgccagtt tgttggcgct gataacgtgg aaaccgtggc
    34741 tggcgaaatc aagatactga tcagcaagct gctggctggc ggtaacgtcc agcaccacta
    34801 aatcatcata cggatgggcg cgcatccaca ggaacaacga ctcttcatcc tgctcaaccg
    34861 cttcatcgtt gaagaaggct aacgcgcggc tggcgtccag cccgtcatag ctcaacaggc
    34921 tacggcggct gtccaccaca cctgccagca caaactcaaa gccggtacgt gccgaaagcg
    34981 tgctctgctc acgggcgaac agttccagcc aacgggaacc gatattgccc ttaccgaaca
    35041 ataccaggcc gatgcgtttt tctgcgcgga agacggactg atgcagcccc tgaatcaggc
    35101 tttcggtcgg gccggtgcgc agtactgcca ccaggctgat gccgtcatcg gactgccagg
    35161 taaattcgac cggctggcct ttcagttgct gccagaagcg gtggcaatgc agcgggttac
    35221 gggtgacgcc tgcaccgacc atcgccacca gcgccagccc ctgacgcagg cgcagttcgc
    35281 caggtaatcc cgcttcgtcg aggattttca gcgcactgtc ggccacttct gaggtgtagc
    35341 aaaattgcag caactggcga tcgttatgta cgccaaccgc cagcgggcgt acctgcgcgc
    35401 gtttcaggat ttggtcgatc tctttatgcg ccagtttgaa atcctgactg gcgggcacct
    35461 gaaactcaat caaacagaca tcatcgtggc tggtgacaat acgcgcacca gtaccggagg
    35521 ccagcacgcg ttcaatgcgc gtggaacctt gatccggcgt gtagctacag cgcagttgca
    35581 ggtcgatttc gctgccagaa accggctgta aagtacgggc gtgaagaacg ggagccgcca
    35641 ggcgcgccag ttcgctggcc tcatccagac gcagcaacgg cagcaggcag gcatctttca
    35701 ctttacgcgg gtcggcactg tataccccgg cgacgtcgct ccagatggtt acgcgagaaa
    35761 cacccgccag cgcaccgatt tgtgtcgcgg aatagtcgga accgttacgc cccagcagca
    35821 ccgtttcacc ggcgttgttg cggctgataa atccggtcac caccagacgt ttgcccggat
    35881 gttgcaccag cagctgttgc agcaacgggt aagaaagccc ttcatcaacc tgcggttgtg
    35941 cggcgcgttc agcgcgtaaa aactcgcggg catcaagcca ggccgctggc agcccttgtt
    36001 gattaagtac cgcagacatc aggcgtgccg accatacttc cccgtggccc accacttccg
    36061 catacactgc gtcgttaata ccgctgtcga gcagcgccgc caggcgctca aggtcgctga
    36121 caaaagcgct aatgaggcta tcagcttctt cagcgggtag cagaccgcta atcagatcgc
    36181 actgatagcg acgcagcgtt tgttgaacct gatgcgcaga gagacgatcg gtctggctta
    36241 gtttcaacca gttaatcaac tggttagtgg tgctaccggc ggcggaaacc accatcatat
    36301 cgtcaggctg agagtactcc gccataatgc ccgcaacacg caaataacac ttcacatcag
    36361 ccagactact gccaccaaat ttatgcagtt gacgaccttt cgcccctgcc tgcgcaatca
    36421 cactcatttt taccccttgt ttgcagcccg gaagccattt tccaggtcgg caattaaatc
    36481 ttcgccatct tcaataccgg tggagatacg cagcagcgtc tcggagatcc cggcggcagc
    36541 acgcgcttct ggtgccatgc ctgcatgtgt catggttgcg gcgtgagaga ttaaactttc
    36601 cactccccct aatgattccg ccagcgtaaa caacgacagc ccgcccagga aacgacgcag
    36661 cgtctgctca tcgccatcca gttcaaaact caacattgcg ccaaagcctt tttgctggcg
    36721 cgcggcaatt tcatgcccct gattttccgg cagcgacggg tgatacagtt ttttcaccaa
    36781 cggctgggtt tgcagatatt tcacaatcgc ctgcgcgttg cgctgggcca gctccatacg
    36841 cggcaccagc gttcgcaacc cgcgtagcag cagatagctg tcaaacgcgc cgcccgtcac
    36901 gccaatattg tttgtccacc aggcaagttc agtgacaacg tccgggtctt tagcaatcac
    36961 cacgccagcc accacgtcag agtgaccgtt cagatatttc gtacatgaat gcaacaccag
    37021 atcggcaccc aatgccagcg gattttgtaa tgccgggctt aagaaggtgt tatccaccac
    37081 gctcaccgcc ccgacttccc ttgccagatg gcagattttc gcaatatcca cgacgcgtaa
    37141 caatggatta cttgggcttt ctaccagtac cagtttaggt ttttctgcca gcgctgcccg
    37201 taatgcctgt tcatcgcctt gatcaacaaa caacacgcga tagcaaccgc gtttcgccag
    37261 actgtcgaac aggcgatagc taccgccgta gcagtcgtgc ggcgcaacca gcagatcgcc
    37321 aggtttcaaa aagacggtcg ttaccaagtg aatcgcggac atgccggtat tagtaagtac
    37381 tgcgccagca ccgccttcca gttctgccag cgcacgctga accacatcgc gcgttgggtt
    37441 gccgcgacgt gagtaatcat gcgcgcgcgg ttcattaaat ccggtaaagt tataggtgct
    37501 ggaaagatgg atgggaggga caacgcaacc atactgttcg tcgtcattta acccgctacg
    37561 cactgcgatg gtggcctgtt tacgcgtcat gtgatgaagt ttcctgggct ttgtcggtga
    37621 aatgtcaggc accagagtaa acattgtgtt aatggacgtc aatacatctg gacatctaaa
    37681 cttctttacg tatagattga gcaaatccca aatagccgtt aaaattatat gcattatcac
    37741 gccgacaggt gcattacacg atgtcacggt aacgcctgta cggtaaacta tgcgggttta
    37801 cggtcagtac ccacataaat tgtgtggtct ggtctcaatt tattgacgaa gaggattaag
    37861 tatctcatgg ctgaatggag cggcgaatat atcagcccat acgctgagca cggcaagaag
    37921 agtgaacaag tcaaaaagat tacggtttcc attcctctta aggtgttaaa aatcctcacc
    37981 gatgaacgca cgcgtcgtca ggtgaacaac ctgcgtcacg ctaccaacag cgagctgctg
    38041 tgcgaagcgt ttctgcatgc ctttaccggg caacctttgc cggatgatgc cgatctgcgt
    38101 aaagagcgca gcgacgaaat cccggaagcg gcaaaagaga tcatgcgtga gatggggatt
    38161 aacccggaga cgtgggaata ctaaagcaaa aaagagcggc gcggagtgga atcgcctgat
    38221 gcgctacgct tatcaggcct acgtcatatt gcaatttatt gaatttgcac gaacttgtag
    38281 gccggataag gcgttcacgc cgcatccggc ataaacaacg agcacgttgt ctgcgaccca
    38341 ccgcttttta tacatggacg tttaactatg aaaaacaggc tgctgatcct cagcctgctg
    38401 gtttctgtac ctgcctttgc ctggcagcca caaaccggcg acatcatctt tcagatctct
    38461 cgctcatcgc aaagtaaagc gatccaactg gcgacccatt ccgattatag ccacaccggt
    38521 atgctggtga tacgcaacaa aaagccctac gtttttgaag cggtcggccc ggtgaaatac
    38581 accccgctca agcagtggat cgcccatggt gaaaagggca aatacgttgt tcgccgcgtt
    38641 gaaggcggac tgagtgttga acaacagcaa aaactggcgc aaacggtaaa acgttatctc
    38701 ggtaaaccgt atgatttcag cttctcatgg agcgacgatc gccagtactg ttcggaagtg
    38761 gtgtggaagg tttaccagaa cgcgctggga atgcgcgtgg gcgagcagca gaagttgaaa
    38821 gagttcgatc tcagcaaccc gctggtccag gctaagctca aagaacgcta cggcaaaaat
    38881 attccgcttg aggaaacggt agtttcacca caagccgttt tcgacgcgcc acaactcacc
    38941 acggttgcca aagagtggcc gctgttttcg tggtaatgga tgatgaaaaa aataattccc
    39001 gccgtagtct gtttgttgct ttgtagttgc acgacctttc cgcaaactgt cgctccggta
    39061 aacggtgatg cctccaatta gttgaattaa ggatataata ctcgctttta aggttgtttc
    39121 atggtcacag ttaaccttca ttgcccccgt tgccagtccg ttcaggttta ccgtcacggg
    39181 cagaacccaa aaagacatga tcgattccgc ggccgtgact gtcaccgggt atttcagctc
    39241 agttactgtt acgagccccg taaaccggga gttaaagacc aaatcgctga aatgactttc
    39301 aacggtgccg gggttcgcga tactgcccgt acgctgaaaa tcggcattaa tacagtcatc
    39361 cgaactttaa aaaactctcg ccacgaagaa taacgtcatc cccggtcgct catgctgatg
    39421 tggcactcat ctgcgaactt agtcagcaat ggagctttgt cggcagtaaa gcgcgacagc
    39481 actggctctg gtacgtgtac aacacgaaaa ctggtggtgt tctggcctac acttttggcc
    39541 cccggactga cgaaacctgt cgtgaattgc tggcgctgct taccctttta ccatcggcat
    39601 gttgaccact gacgaatggg gtagttacac cagagagtta ccgaaggaaa agcatctgac
    39661 tggtaagatt tttactcagc gcattgagcg caacaatctg accctgagaa cccgaatcaa
    39721 acgtctggcc cgcagaacca tctgtttctc gcgctctgta gaacttcatg aaaaagtcat
    39781 cggtgctttt atcgaaaata tatgttctac taattggagt catcaccctt ctgatgagct
    39841 ttccttggat gattaccaga ctgaatacac agccaaagat ttttacctca ttctgctctt
    39901 ttttctcaaa tgacatatat tatattttac atcatattca taggcatatg acattccccc
    39961 gcccccatta tgtcattgtg ggttgatgtt gggctttcat aaaaaaacat ctttttttga
    40021 actagcatta attatattta ctttatatat taaaatattt tctccatctg gaaatatttt
    40081 agccacacaa tataaagcag ctgaatccga attacttaaa aatatattat cattacccat
    40141 aactccagcc attaaattac tggagactaa agcaaggaaa taacaacatt gtattgcctt
    40201 cattttaatt actcccttgt ctgataattg taactccatt ccctttagct ttgggattat
    40261 attttagata ggtgttattt ggtgttccca agtaactact tgtattcttt ttttcccatt
    40321 ccattcatat aagaattcgt taaatctttg cttgaaaaat ttagactatc ataaacatca
    40381 ttttcttttg ttactttatt tcctttatcg tcagagtaaa aaccgtgagt gtgataatcg
    40441 cctactctct ctgaaccttc agggcatttt atatttcgca agtctacact atcattatca
    40501 tttgaactta tcggattcat ggggaaatat tcaccttgct tcttacatat tagtccacca
    40561 tattctttat tctcgttaat tgattctcca ttacacat
//
LOCUS       DAJTOR010000012        40418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTOR010000012 DAJTOR010000000
VERSION     DAJTOR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.012"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(115..2436)
                     /locus_tag="OO249_000604"
     CDS             complement(115..2436)
                     /locus_tag="OO249_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5635008.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFITRYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2533..3009)
                     /gene="greB"
                     /locus_tag="OO249_000605"
     CDS             complement(2533..3009)
                     /gene="greB"
                     /locus_tag="OO249_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5635009.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            3237..3956
                     /gene="ompR"
                     /locus_tag="OO249_000606"
     CDS             3237..3956
                     /gene="ompR"
                     /locus_tag="OO249_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5635010.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3953..5305
                     /gene="envZ"
                     /locus_tag="OO249_000607"
     CDS             3953..5305
                     /gene="envZ"
                     /locus_tag="OO249_000607"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5635011.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5381..7003)
                     /gene="pckA"
                     /locus_tag="OO249_000608"
     CDS             complement(5381..7003)
                     /gene="pckA"
                     /locus_tag="OO249_000608"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5635012.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7382..9106
                     /locus_tag="OO249_000609"
     CDS             7382..9106
                     /locus_tag="OO249_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5635013.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNCSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDVTFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9241..10119)
                     /gene="hslO"
                     /locus_tag="OO249_000610"
     CDS             complement(9241..10119)
                     /gene="hslO"
                     /locus_tag="OO249_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5635014.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10144..10545)
                     /gene="hslR"
                     /locus_tag="OO249_000611"
     CDS             complement(10144..10545)
                     /gene="hslR"
                     /locus_tag="OO249_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5635015.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10556..11224)
                     /gene="yrfG"
                     /locus_tag="OO249_000612"
     CDS             complement(10556..11224)
                     /gene="yrfG"
                     /locus_tag="OO249_000612"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5635016.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11289..13424)
                     /gene="igaA"
                     /locus_tag="OO249_000613"
     CDS             complement(11289..13424)
                     /gene="igaA"
                     /locus_tag="OO249_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5635017.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRSQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDCHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13744..14304
                     /gene="nudE"
                     /locus_tag="OO249_000614"
     CDS             13744..14304
                     /gene="nudE"
                     /locus_tag="OO249_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5635018.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14469..17021)
                     /gene="mrcA"
                     /locus_tag="OO249_000615"
     CDS             complement(14469..17021)
                     /gene="mrcA"
                     /locus_tag="OO249_000615"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5635019.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17141..17920
                     /gene="hofM"
                     /locus_tag="OO249_000616"
     CDS             17141..17920
                     /gene="hofM"
                     /locus_tag="OO249_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5635020.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALFVDPDS
                     VAICGEGGFDPWEAVSVRQPLLPPPGGDFAIALGLALGKAY"
     gene            17920..18459
                     /gene="hofN"
                     /locus_tag="OO249_000617"
     CDS             17920..18459
                     /gene="hofN"
                     /locus_tag="OO249_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5635021.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRKQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18443..18883
                     /gene="hofO"
                     /locus_tag="OO249_000618"
     CDS             18443..18883
                     /gene="hofO"
                     /locus_tag="OO249_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5635022.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18873..19277
                     /gene="hofP"
                     /locus_tag="OO249_000619"
     CDS             18873..19277
                     /gene="hofP"
                     /locus_tag="OO249_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR5635023.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEQPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19189..20427
                     /gene="hofQ"
                     /locus_tag="OO249_000620"
     CDS             19189..20427
                     /gene="hofQ"
                     /locus_tag="OO249_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5635024.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGIEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            20828..21349
                     /gene="aroK"
                     /locus_tag="OO249_000621"
     CDS             20828..21349
                     /gene="aroK"
                     /locus_tag="OO249_000621"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5635025.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            21406..22494
                     /gene="aroB"
                     /locus_tag="OO249_000622"
     CDS             21406..22494
                     /gene="aroB"
                     /locus_tag="OO249_000622"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5635026.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            22586..23872
                     /gene="damX"
                     /locus_tag="OO249_000623"
     CDS             22586..23872
                     /gene="damX"
                     /locus_tag="OO249_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5635027.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGDKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            23979..24815
                     /gene="dam"
                     /locus_tag="OO249_000624"
     CDS             23979..24815
                     /gene="dam"
                     /locus_tag="OO249_000624"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5635028.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            24833..25510
                     /gene="rpe"
                     /locus_tag="OO249_000625"
     CDS             24833..25510
                     /gene="rpe"
                     /locus_tag="OO249_000625"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5635029.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            25503..26261
                     /gene="gph"
                     /locus_tag="OO249_000626"
     CDS             25503..26261
                     /gene="gph"
                     /locus_tag="OO249_000626"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5635030.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            26254..27258
                     /gene="trpS"
                     /locus_tag="OO249_000627"
     CDS             26254..27258
                     /gene="trpS"
                     /locus_tag="OO249_000627"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5635031.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            27527..28432
                     /locus_tag="OO249_000628"
     CDS             27527..28432
                     /locus_tag="OO249_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635032.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            28449..28811
                     /locus_tag="OO249_000629"
     CDS             28449..28811
                     /locus_tag="OO249_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5635033.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            28895..30058
                     /locus_tag="OO249_000630"
     CDS             28895..30058
                     /locus_tag="OO249_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5635034.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            30058..31284
                     /locus_tag="OO249_000631"
     CDS             30058..31284
                     /locus_tag="OO249_000631"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5635035.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            31281..32159
                     /locus_tag="OO249_000632"
     CDS             31281..32159
                     /locus_tag="OO249_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5635036.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            32170..32523
                     /locus_tag="OO249_000633"
     CDS             32170..32523
                     /locus_tag="OO249_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5635037.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            32535..33839
                     /locus_tag="OO249_000634"
     CDS             32535..33839
                     /locus_tag="OO249_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5635038.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            33851..34936
                     /locus_tag="OO249_000635"
     CDS             33851..34936
                     /locus_tag="OO249_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5635039.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGEVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35088..35819)
                     /gene="phoB"
                     /locus_tag="OO249_000636"
     CDS             complement(35088..35819)
                     /gene="phoB"
                     /locus_tag="OO249_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5635040.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCKKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(35919..36704)
                     /gene="frlD"
                     /locus_tag="OO249_000637"
     CDS             complement(35919..36704)
                     /gene="frlD"
                     /locus_tag="OO249_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5635041.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(36701..37531)
                     /gene="frlC"
                     /locus_tag="OO249_000638"
     CDS             complement(36701..37531)
                     /gene="frlC"
                     /locus_tag="OO249_000638"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5635042.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNIVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(37581..38603)
                     /gene="frlB"
                     /locus_tag="OO249_000639"
     CDS             complement(37581..38603)
                     /gene="frlB"
                     /locus_tag="OO249_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5635043.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQAAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(38624..40012)
                     /gene="frlA"
                     /locus_tag="OO249_000640"
     CDS             complement(38624..40012)
                     /gene="frlA"
                     /locus_tag="OO249_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR5635044.1"
                     /translation="MTANSPLQRVGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLTFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISDLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGLFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            complement(40257..>40418)
                     /locus_tag="OO249_000641"
     CDS             complement(40257..>40418)
                     /locus_tag="OO249_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5635045.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
BASE COUNT         9345 a        10252 c        11117 g         9704 t
ORIGIN      
        1 tgtggaataa aaaggccgga gatattctcc ggcactttta tgtcaggcaa ggcgttcgtt
       61 gccagatgcg gcgtgaacgc cttatcgcgc aggtttaggg gccagtccgg gcgtttaacg
      121 ttttttgccc attgccgccg ccagcgcatc catcatcgcg ctattaccgg caggctgcgc
      181 ttcacgaccg cgtggtttgg ctgccgggcg gttgttttgc gggcgttcat taccgccgcc
      241 gcgacgggcg ttggtttcgc caggctgctc gtccagacgc atagtcaggg cgatacgttt
      301 acgctgaaga tccacttcca gcactttcac cttcacaatg tcgcccgctt tcaccacggt
      361 atgcggatct tccacaaact tgttcgacaa tgaagagatg tgaaccaggc cgtcctgatg
      421 cacgccgata tcgacaaacg cgccaaagtt ggtgacgttg gtcaccgcac cttcgaggat
      481 catacccggt tgcaggtcgt tcattgtctc gacgccatcg gcaaactgag cggttttaaa
      541 ttccggacgc ggatcgcgac ccggtttttc cagctctttg atgatgtcag ttactgtcgg
      601 cacaccgaat ttttcatcag taaagtcaga cgctttcagg ttacgcagtt cgctgctgtt
      661 acccatcaga tctttcagtg cctgctgtgt tgctgccaga atgcgttcca ccaccggata
      721 ggcttccggg tggacggtag aagcatccag cgggttatca ccgtggttaa tgcgcaagaa
      781 gcccgcgcac tgctcgaagg ctttcggccc cagacggctg actttcagca gttgctgacg
      841 gttctggaac tggccgttct catcgcgcca ggccacgatg ttttgcgcca tcatgcgcgt
      901 caggcccgcc acgcgggtta acagcggaac agaagcagtg ttgagatcga cgccaacggc
      961 gtttacgcag tcttctacta ctgcgtccag tttgcgggcc agttgcgtct ggctgacgtc
     1021 atgctgatac tgacctacgc cgatagattt cggatcgatt ttcaccagct ccgccagcgg
     1081 atcctgcaaa cggcgggcga tagacaccgc gccacgcagc gaaacgtcga gatccgggaa
     1141 ctcctgcgct gccagctcgg aagctgagta aaccgacgcg ccagcttcgc tgacgatcac
     1201 tttctgtgcg gtcactttcg ggaactgctt ctgcacgtcg agatagaaac gttcagtttc
     1261 gcgggaagct gtaccgttgc cgatcgccac cagttcaacg ttatgttttt cacacaaggc
     1321 agcaacggtc atcgctgctt ttgcggcttg tccggtgtgc gggtaaatgg tatcggtcgc
     1381 taccagtttg ccagtggcat cgaccaccgc cacttttacc ccagtacgca gacccggatc
     1441 gaggcccatc gttgcacgca gtccggcagg ggccgccatc agcagatcgt gcaggttacg
     1501 ggcaaagacg ttgattgctt catcttccgc gcgttcgcgc acggtaccca tcagttcggt
     1561 ttccagatgc atcagcacct tgatgcgcca ggtccagctc actacgcctt tgcgccagct
     1621 atccgccggg gcattgttca ggcgcaggcc aaggtgatcc atgatgattt gctcgcaata
     1681 gctctctttg ggcggctcat cgaactgcgg atcggcattc agcgaaagct ggagtacgcc
     1741 ttcgttacgc ccacggaaca tcgccagagc gcggtgagaa ggcaccgtgg acaacggttc
     1801 gtgatgatcg aaatagtcgc ggaatttcgc cccttcctct tctttaccgc tcaccaccgt
     1861 agaaaccaaa tgcgcgttct tccacagata atcacgcact ttcgccagca gcgcggcatc
     1921 ttcggcaaac cgttccatca ggatatagcg cgcgccatcc agcgcggctt tggtatctgc
     1981 cacgccttta tcggcatcaa catattgtgc agcggcgact tctggcgtgt gtgacggatc
     2041 gctccacagc aggtcagcca acggctcaag ccctgcttca atggcgattt gcccgcgggt
     2101 gcggcgttta ggtttgtagg gcaggtagag gtcttcgagt tcggttttgc ttagggtggc
     2161 gttgatggcc ttcgccagat catcggtgag tttgccttgc tcggaaatgg acttgaggat
     2221 cgcctgacgt ctctcttcca gctcgcgcag atagctcaga cgcgtttcca gattacgcag
     2281 ctgcgtgtca tccagaccgc cggtgatttc cttacgataa cgtgtgataa acggcacggt
     2341 attcccttcg tcaagcaggc gaacggcagc gtcaacctgt tccgggcgcg cctgaatttc
     2401 acccgcaata atgcggcaga acgaatcatt catcatggtt tgagttcatc ttttcggatc
     2461 aaaaatcagg ggatagttat acggactggc tggcaaaaat gccagccatc ggcaggaggt
     2521 taagactctt ccttacggtt tcacgtactc gatagcatta acataccagc tcgcttcccc
     2581 ggcaggggta ttcaccaccg ccagatcgcc gacttctttt ttcagcaatg cgcgggccat
     2641 cggggaatcg atagagatgt aatctttacg gccaaaaatt tcatcgtagc cgacaatacg
     2701 gaaacggtga gtcacgccat cgtcgttttc aatctccacc cacgcgccaa aaaagacttt
     2761 gccttcctgc tgaggggagt aatcgacgat tttgagattt tccatgcatt tagtgagata
     2821 gcgcacgcga cggtcgattt cacgcagacg ctttttatta tactgatagt cagcattttc
     2881 gctgcggtcg cccagacttg cggcccaggt cacctttttt gtgacctccg ggcgttcttc
     2941 acgccagaga taattaagct cttgtttgag tttttcatac ccttcccggg taaccagggg
     3001 cgttttcatc tcgttgattc cctttgtctg tttgataatg cgcacattgg gtataacgtg
     3061 atcatatcaa cagaatcaat aatgtttcgc cgaataaatt gtatacttaa gctgctgttt
     3121 aatatgcttt gtaacaattt aggctgaaat tcataccaga tttagctggt gacgaacgtg
     3181 agctttttta agaatacacg cttacaaatt gttgcgaacc tttgggagta caaacaatgc
     3241 aagagaacta caagattctg gtggtcgatg acgacatgcg cctgcgtgcg ctgctggaac
     3301 gttatctcac cgaacaaggc ttccaggttc gaagcgtcgc taatgcagaa cagatggatc
     3361 gcctgctgac tcgtgaatct ttccatctta tggtactgga tttaatgtta cctggtgaag
     3421 atggcttgtc gatttgccga cgtcttcgta gtcagagcaa cccgatgccg atcattatgg
     3481 tgacggcgaa aggggaagaa gtggaccgta tcgtaggcct ggagattggc gctgacgact
     3541 acattccaaa accgtttaac ccgcgtgaac tgctggcccg tattcgtgcg gtgctgcgtc
     3601 gtcaggcgaa cgaactgcca ggcgcaccgt cacaggaaga ggcggtaatt gctttcggta
     3661 agttcaaact taacctcggt acgcgcgaaa tgttccgcga agatgagccg atgccgctca
     3721 ccagcggtga gtttgcggta ctgaaggcac tggtcagcca tccgcgtgag ccgctctccc
     3781 gcgataagct gatgaacctt gcccgtggtc gtgaatattc cgcaatggaa cgctccatcg
     3841 acgtgcagat ttcgcgtctg cgccgcatgg tagaagaaga tccagcgcat ccgcgttaca
     3901 ttcagaccgt ttggggcctg ggctacgtct ttgtaccgga cggctctaaa gcatgaggcg
     3961 attgcgcttc tcgccacgaa gttcatttgc ccgtacgtta ttgctcatcg tcaccttgct
     4021 gttcgccagc ctggtgacga cttatctggt ggtgctgaac ttcgcgattt tgccgagcct
     4081 ccagcagttt aataaagtcc tcgcgtacga agtgcgtatg ttgatgaccg acaaactgca
     4141 actggaggac ggcacgcagt tggttgtgcc tcccgctttc cgtcgggaga tctaccgtga
     4201 gctggggatc tctctctact ccaacgaggc tgccgaagag gcaggtctgc gttgggcgca
     4261 acactatgaa ttcttaagcc atcagatggc acagcaactg ggcggcccga cggaagtgcg
     4321 cgttgaggtc aacaaaagtt cgcctgtcgt ctggctgaaa acctggctgt cgcccaatat
     4381 ctgggtacgc gtgccgctga ccgaaattca tcagggcgat ttctctccgc tgttccgcta
     4441 tacgctggcg attatgctat tggcgatagg cggggcgtgg ctgtttattc gtatccagaa
     4501 ccgaccgttg gtcgatctcg aacatgctgc tttgcaggtc ggtaaaggga ttattccacc
     4561 gccgctgcgt gaatatggcg cgtccgaggt acgttcggtt acccgcgcct ttaaccatat
     4621 ggcggctggt gttaagcaac tggcggatga ccgcacgctg ctgatggcgg gggtaagtca
     4681 tgacttacgc acgccgctga cgcgtattcg cctggcgacc gagatgatga gcgaacagga
     4741 tggctacctg gccgaatcga tcaataaaga tatcgaagag tgcaacgcca tcattgagca
     4801 gtttatcgac tacctgcgca ccgggcagga gatgccgatg gaaatggcgg atctcaacgc
     4861 agtactcggt gaggttattg ctgccgaaag tggctatgag cgggaaattg aaaccgcgct
     4921 ttaccccggc agcattgaag tgaaaatgca cccgctgtcg atcaaacgcg cggtggcgaa
     4981 tatggtggtc aacgccgccc gttacggcaa tggctggatc aaagtcagca gcgggacgga
     5041 gccgaatcgc gcctggttcc aggtggaaga tgacggtccg ggaattgcgc cggaacaacg
     5101 taagcacctg ttccagccgt ttgtccgcgg cgacagtgca cgcaccatta gcggcacggg
     5161 attagggctg gcaattgtgc agcgtatcgt ggataaccat aacgggatgc tggagcttgg
     5221 caccagcgag cggggcgggc tttccattcg cgcctggctg ccagtgccgg taacgcgggc
     5281 gcagggcacg acaaaagaag ggtaaataaa cgggaggcga aggtgcctcc cgttttgctt
     5341 tctataagat actggataga tattctccag cttcaaatca ttacagtttc ggaccagccg
     5401 ctaccagcgc ggcacccgca ggggtgtcgg tgtatttatc gaagttgtcg ataaacagtt
     5461 tcgccagggt ttcggctttt tcctgccact gttccggaga agcgtaggtg ttacgcggat
     5521 cgagaatctt cgtgtctacg cccggcagtt cggttgggat cgccaggtta aacatcggca
     5581 gagtgaaggt ttctgcatta tccagcgaac cgttgaggat ggcgtcgata atggcgcggg
     5641 tatctttaat cgagatacgt ttgcctgtgc cgttccagcc agtgttaacc agataagcct
     5701 gcgcgcccgc cgcctgcata cgtttcacca gcacttctgc gtactgagtc gggtgcagcg
     5761 acaggaatgc cgcgccgaag caagcggaga aggttggcgt cggttcggtg atgccacgct
     5821 cagtaccggc cagtttggcg gtgaagccag agaggaagtg atactgggtt tgatcggcag
     5881 tcaggcgaga aaccggcggc aacacgccga aagcatcagc agtcaggaag ataaccttag
     5941 tcgcgtggcc cgctttggaa accggcttaa cgatgttatc gatgtgatag attggataag
     6001 aaacgcgggt gttctcggtt tttgaaccat catcaaagtc gatagtgcca tcttcacgca
     6061 cggtgacgtt ttccagcagc gcatcacgac ggatagcgtt gtagatttca ggttccgctt
     6121 ctttcgacag cttgatagtt tttgcgtagc agccgccttc gaaattaaac acgccgtcat
     6181 cgtcccagcc gtgttcgtca tcgccaatca ggcggcgttt cgggtcggtg gaaagggtgg
     6241 ttttaccggt gccggaaagg ccgaagaaca ccgcaacatc gcctttctcg ccaacgttgg
     6301 cggagcagtg catagaagcg atacctttca gcggcagcag gtagttcatc atcgagaaca
     6361 tccctttctt catttcgccg ccgtaccagg tgccgccaat cagctgcatg cgctcggtca
     6421 ggttaaacgc cacgaagttt tcggagttca gaccctgttc tttccactgc gggttagtgc
     6481 acttcgcgcc gttcataacg ataaagtctg gtttgaaacc tgccagctct tcatcgctcg
     6541 ggcgaataaa catgtttttg acaaaatgcg cctgccaggc cacttcggtg atgaaacgga
     6601 cggaaagacg agtatccggg ttcgcaccac agaaagcgtc gacaacgaac agacgtttgc
     6661 cggaaagctg cctggtcacc aggcctttca gatgctgcca ggtttccgga gagagaggtt
     6721 tgttgtcgtt cttacctttg cctttgtctg cccaccagaa agtatcgcga gtggtatcgt
     6781 cacggacgat atacttatct tttggtgaac gaccggtgaa gatcccggta tcgacggcaa
     6841 cggcacccag attagttaac accccgcgct cataacctgt caggctcgga tcgagctctt
     6901 cctgatacag caggtcgtag cttgggttgt aaacgatatc atgtacgtca ctgataccat
     6961 aagcctcgag ttcttgcggg gtcaaaccat tgttaacgcg catttcactg ctccttagcc
     7021 aatatgtatt gcctgaatag taaagtcttt ttgggggtgt taaccgcgac aaggctcata
     7081 gatttacgta tctggagaaa ttcatatttg acaaaaaacc ccgtgattcc tgttacgaaa
     7141 cggttgctat tatcgcagaa atagcatttt aggtgtggaa aatgttctca aaaggttaaa
     7201 tcttggttta tatggcggga atattgtgag tggaatcgca ttccttaatg aaattgatgc
     7261 aaattcaatc ggtaaagtat cgattcacca cgctaacttt ttttgattat tttctgaaaa
     7321 aagcaaaata ctactgcccg gatgcgataa ttaacccacc ttttgattct ggataaccaa
     7381 catggataac gttgaacttt caccggcaac acgctggggg atgattgcta ccggattact
     7441 tcaggggctg gtttgctacc tgttgatcgc ctggcttgcc ggaaaaaatc acagttggat
     7501 tgtttatggc gtgcctgcga ccgttgcgtt ttcatccgtt ttactcttct ccgtgatctc
     7561 ttttaaacag aagcgccttt ggggatggct ggcgctggtg tttattgcca cgacagggat
     7621 gagcggttgg ctgaagtggc aaactgatgg catgaacccc tggagagctg aaaaggcgat
     7681 ttgggatttt ggctgctatc tgctgttgat ggcaatgttg ttgctaccgt ggatacaaca
     7741 aagccttcgt atccgcaatt gcagtagccg ctatagctac ttttaccagt cagtatggca
     7801 taacgtactt atattactgg tgatttttct cgccaatggc ctgacgtggc tggtgctttt
     7861 actgtggggt gagttgttta aacttgttgg catcaagttt ttcaatacgc tcttttttgc
     7921 aaccgactgg tttatgtatc ttacattagg tctggttacc gcactggcgg tgattctcgc
     7981 gcgcacacag tcacgtttaa tcgactctat tcaaaagttg ttcacgttaa tcgccacggg
     8041 gttgctgccg ttagtatcat tgctaaccct gatgtttatc atcactctgc cgtttacggg
     8101 cctgagcgcg atttctcgcc acatctccgc cgccgggttg ctgttaacgc tggccttttt
     8161 gcaattgatc ttaatggcta ttgtccgcga tccgcaaaaa gcgtcacttc cctggacggg
     8221 gccgttgcgt tgcctgatta aaaccgcttt gctggttgct ccgctgtatg tgttcgtcgc
     8281 cgcctgggcg ttatggctgc gggtcgctca gtacggctgg actgtcgacc gcttgcaggg
     8341 cgcgctggcg gtgctggtgt tactggtgtg gtcgctgggg tattttgtca gtatcgtctg
     8401 gcgtaatggg caaaatcccc ttgttcttca gggcaaagtg aaccttgcgg tttcgttatt
     8461 ggtgttggtg atactggtgc ttcttaattc gccggtgctg gacagtatgc gtattagcgt
     8521 gaacagtcat atggcgcgtt atcagagcgg caaaaacacg ccagaccagg taactatcta
     8581 catgctcgaa cagagcggtc gctatggacg tgcggcgctt gagtcgctga aaagcgatgc
     8641 cgggtttatg aaagacccga aacgcgcgcg ggatctgctg atggcgttag atggagagca
     8701 acatcttcag gaacaggtat cggaaaaagt attagccgaa aatgtgttaa ttgcccctgg
     8761 ttctgttaaa ccggatgtga cattctggtc ggccttaatc caggatcgct ataacgtgat
     8821 gacctgtatt gaaaaagacg cctgcgtcct ggtcgagcaa gatctgaata gtgatggtca
     8881 ggcggagcgg atcctgtttg cttttaatga tgacagagtc attgtctatg gctttgactc
     8941 agacagaaaa gaatgggacg cgcttgatat gagtttactt ccgaacgaaa taacgaaaga
     9001 aaaattactc acagctgcga aggatggaaa actggggacg aggccgaaag cgtggcgcga
     9061 tctgacggta gatggtgaaa cgctggaaat aaacttaagt aagtgacaac gcttttatcg
     9121 gatacgacgc tgtatctgac caaaataatt tgcgatttat ttcataggcg ctggtaagcg
     9181 cagcacatca ggccattaag ttgtaaaatc cggcagagcc ctctctgccg gacatactca
     9241 ttaatgaact tgcggatctg ccggagacgc gttgttgcgg atttcagcaa tatccatcgc
     9301 attgaacaga tagtggttac cgcagtaatc acaatgcatg tcaatttcgc catcttccgc
     9361 caggatgcta tcaacttctt catcaggcag cgttttcagc gcatcggcgc aatgttcacg
     9421 cgagcaggtg catttgaact ccacatcctg cggatcgtaa accgtcacct cttcttcgtg
     9481 atacaaacgc cacaacactt cgtttgccgg taaggtcagc agttcttcgg ttttgatggt
     9541 ttcggttagc gtcgccaggt ggtcaaagtc gtcctgctgg gcattttgcg caggcattac
     9601 ctgcaacaac ataccgcctg cagccggttt gccgtctacg tcgccggtgc gaataaacag
     9661 gcgcgtcggc agctgttcag aacgcataaa gtaatcttcc aggcaggccg ccagggtatc
     9721 accttccaga ccaactacgc cctgatagcg ttcgccttcg ctcggggtaa tggtgatcac
     9781 cacgtaacca ttgccgacca gcgttttcag gtcggcattt tctggaattt cgccctgcac
     9841 gcgcgccaca ccgcgcattt gctggttatt gttaccgtta ataaccgcca gattcatcgg
     9901 accgtcgccc tgcagctgta cggtgatatc accatcaaac ttcagcgtag cggttaacag
     9961 gctggtcgca accagcagtt ctgccagcac gtttttaacg ggctgcggat aatcgtggtt
    10021 ctcaaggatc tgttgcaggg tttccgaaac ggttaccagt tcgccgcgaa cggcaaaatt
    10081 ttcaaacaga tagcgatgta attggtcatg ttgcggcata atcatctctc ttgcaggtga
    10141 cagttattca ctgtcgccgt gtttaaatcg taacaggtcg cggcgctctt ttttgtccgg
    10201 gcgtcggtcc gggtgcggca tggttaaggc attaagttta cgtgccatcg ccattttttc
    10261 gcgtttctct acactttccg cagtctcttc atacagcaag gctgcctcgc tggcggggcg
    10321 acgctgttca gtaatcgcct ttacaatcac cgtgcgttcg tcatttccct ggcgcagagt
    10381 gagcgtggca ttcagctcga cgattttgct cggcttgctg cgctgcccgt tgtaatgcac
    10441 cttaccgcct tcaatcattt cacgggctag cgcgcgggtt ttataaaaac gggcagccca
    10501 tagccattta tccagtcgaa cctcaacagc aggtttctct ttcatggcgt ctccttcaca
    10561 ttagcgaggg gatcaggcgg cggtagtcat tcagtgacgg atggcgttga tactgtttct
    10621 cggcaatccc ggaatcagga ttagtcacgc cgaggcagta acgaatacca aattgcgcgg
    10681 cagcatcgag aatcgcttcg ctgtcatcaa taaacagcgt tctttcagct ttcagacccg
    10741 tagcttcggc caccgcatgc cataaccgct gatcctcttt cggataacca aatgtgtggg
    10801 tggaaagtaa taaatcaagg tgtgcgtcca gacccgtatg ctcaagtttt accgccaggt
    10861 tgtgcggatg agcattggtg agcaaaattc gctgcttacc gctggctttc agtgcctcaa
    10921 gaaacggaat ggtatcttca cgcagtacgg cgcgcggtcc catctcggtg gtcattgcac
    10981 agatatccag acccagttgc tcactccagt aatcaagaca gtaccagttt agcgtatgct
    11041 gtacgtcgtg atattgctgg cgcatatatt ccatcgcttc ctgtggcgta accccgtttt
    11101 tcgcgcccca tgtttcaggc accagctttt gccagaaata gttatcgaag gcgaggtcga
    11161 gcaacgtgcc gtccatatcc agcagaacgg tatctacgtc ctgccaggca atgttgatat
    11221 gcatgaggga aatctccaga gtgaagcaat ttgcgcgaca gggtagcata acctgtcgcg
    11281 caaacgtgtt attcgataag gctttctgaa ggggtgatca gttgcgggtt caggcagctt
    11341 tcataatagg cctgaatctc catcatgcga gtgcgatgac gctggtaacg acgccaggcc
    11401 tgtacgccat tgtaaatggc gctacccagc atcgtcagta gcagcaatgt ggtgctgaga
    11461 tagcgccaca ggccggaacg atccgggatc ggatgaaggc caatatgctg cgtaccattg
    11521 gcgtcagtga agatttttgt gacgatacct tcggcgttaa acggcgtatg catcagcatt
    11581 tgtgccagtt tctggaaagc gttccactgt tcttgcggcg ggtagtcgta aagtgatgcc
    11641 gaaggccagg gctgatcaac aaaatcgctg ccttcgtcgc tgacaatcag gaatccgccg
    11701 ggggccggac tgtttagtga ttgtgccgct cgcgccgttt catgcgtgat aaacggcgcg
    11761 gtggaggttg ccaccaggtt atccagcgat tccgcactca ccgggcgtaa taacacattc
    11821 acgccatcga gcttcccggc gttggcgcgt tttaccagcg cgtcccagtc tttactgttg
    11881 ccgagattga ccagcgcatt tttcagtcgc acacagtcat ctttggcaga acataaatcc
    11941 gctgtcttca gtacaatgtc gccaaaatca tcaagcaata ccatgccgga tttttgaatc
    12001 gctgaacgta atgaggcact gacgcgagat tcatcttccg gtttagggtg cagctggcga
    12061 ttaactgctt cagtcaatgc cgtcgctttg ttgaccagtt cagattctgg taatggtaat
    12121 gagcgggcgt cattccagat gatctgcgag cagtcaaacg gtaaaaaagg tgaattggtt
    12181 ttcgcgctcc aggttccgga agtgcgaata ttacacattc ccgtaccgct aatacgcaat
    12241 gtatcgccta cccgcacgcc agcgtcagcc agttgtttta cgctggtggc ttcaatggtc
    12301 tgcgcgcctt tcatccatga gagagtgaat ttcagcggca tatccagcgg gatccagaat
    12361 aacagcataa acagcaccag cagcgagccc gcagcgataa tcgtactgcg cagccagtgc
    12421 tgtaacggaa agttttttac ttcatcatgc agcgaaagat atcgcccctg acgcactacg
    12481 tggcagtcga gatagatatc aatatcggtt tgttgatcga gatcctgagc aatgtatggc
    12541 tgccagtgtg cgggatagac caggtcgata ataccgagtg aaatattgtt gatctgttcc
    12601 tgatcgtttt cgccaaacaa tccccaacga cggggtgtac cgcgcagaca atgaatttcc
    12661 cgcagggagg attttgccgg gggcgcgaat agcccccaca gacctgcgcc cagcagcagt
    12721 aatgcgccgc ctgccagcca cggaacaaat acatccgggg taatcaggca gaaaaagaac
    12781 atcaggaagg aggcgacgat cagcaacgct tcacgcagcc cgcgcggacg actcagcgca
    12841 tattcttcat gcgtttcttt gcgaatattg agtagctcga tttgctcact ctcttccccg
    12901 cgaatcgacg cctgcgtgga cggaacaggt tgcaaggcat agccgcgcgt ttcctgcatg
    12961 tactcctgta gcgtatgacc gttgagtgaa ataaccaacg gcagcgaatc ggtatgaatc
    13021 agttcaacgg tattctcatc gttgatgtac tgttcccaga aggggggcag gtggacttct
    13081 accgaatcga ggtagtaacg ccatttattc ggatcgtcgg tagagatgcc gtaacgtgtg
    13141 atagcgtgtg tcagcatcat gacgttgttg ctttcggcat tcagcgccag agagatcggt
    13201 gccgcgctag ctcccgttgg gccaggcacc tgtaatacct gcgtcaggct ctcaagataa
    13261 ttttcaacgg cgctacgttc ttcaggtgtg agtttacgcg tttgcgcatc cgcgaaggcg
    13321 ttggtccagg gcagctgact ccgtctggat cgcactttta tcagccatcc cgcaagtagt
    13381 gagcaggcca gcaaagcagc taaaaaaatc acaatggtgc tcatgctttc cccatcttac
    13441 ttagtctgtc aggcgtggtc agtcgcacca gacacaatac atgattctgc ggttggctat
    13501 cggcaagcgt ggagtttaac ttgagcatca ttaagcacat cacagtaacc gctgatcata
    13561 gcaaaccctc tgaaggcgga atatcagcaa attcctagag ttatttcaac ctattgactt
    13621 ttaatttgat ttgaggacga ttatttatta agttacataa atgtaaataa catgcaattc
    13681 acattgccga aagcgtgtcc gatatcgcac aataaccgct gatttcacag catagatccc
    13741 acgatgagca aatcattaca aaaacccacc attctgaatg ttgaaactgt agcccgttcc
    13801 cgactgttta ccgtcgagag cgtggatctg gagttcagca atggcgtgcg gcgtgtttat
    13861 gaacgaatgc gtccaaccaa ccgggaagca gtgatgattg tgccgattgt ggacgatcac
    13921 ctgatcctga tccgcgaata cgcagtggga actgaatcct acgaattagg tttttcgaaa
    13981 ggattaattg atccgggtga aagcgtctac gaagccgcta accgcgagct aaaagaagag
    14041 gttggatttg gtgcgaacga tctgactttt ttgaagaagc tcagcatggc accgtcttac
    14101 ttttccagca aaatgaatat cgtggtcgcg caagatctct acccggaatc gctggaaggc
    14161 gatgagccag agccgctacc acaggtgcgc tggccgctgg cgcatatgat ggatttgctg
    14221 gaagaccctg acttcaacga agcgcgtaat gtcagcgcgc tgttcctcgt gcgcgaatgg
    14281 ttgaaagggc aggggcgagt gtaaatgcgt gaagtttgcc tgatgcacaa cgcttatcag
    14341 gcttatgggg ttcctgcaat atattgattt ttcacgattt tgttagccgg gtaacgcggt
    14401 cacccgcatc cagcataaac aaatgcactt tgtcagccaa ctgaaaaggc gccgaagcgc
    14461 ctttttaatc agaacaattc ctgtgcctca ccattatcga taatggtcgt tcccacctcg
    14521 tgcaccgcct gttgtgtcgg ctgcgtacct tcgatgaaat actcttcgcg gctgttgcca
    14581 ccattagcta actgcccggt gctgcgatcg atattcaccg tcacaatacc cggtggcggc
    14641 gtcagcggct gctccggcac accttcaaga acggctttca tataagcgtc ccatgcaggc
    14701 tgggcactct tggctccgcc ttcgtaacct gagatctgat ctttaatcgc tccggaagcc
    14761 gttgtatgac cgagattacg acggtgatca tcaaagccaa tccagaccga ggtcacaacg
    14821 cccggaccgt aacccgagaa ccacgcatct ttcgaactgt tagtggtacc ggttttcccg
    14881 ccgatatcgc gacgctgcaa atcacgacct gcacgccagc cagtaccctg ccagcctggc
    14941 tcaccaaaga tattggtgtt caaagcactc ttaatcagga atgccagcgg agtgttgatg
    15001 acgtgcggtg cgtactcctg cgcgccagtc ttcgccacta acgcctgatt tgcctgctcc
    15061 agctgcggca ttggtacaga aacattctgc tgctcgcggg agatagcgac atcttcaaca
    15121 tcgttatttt ccagcacgtt cgatttctgc gtatcaccgt aaatcaccgg aatatcgcat
    15181 tccgggcagg ctactttcgg tttcgcttcg aaaatcacgc cgccctgatc gttttcaatt
    15241 ttgctgataa accacgggtc caccaggaag ccgccgttcg ccatgaccgc gtagccgcgc
    15301 gccacctgca ttggggtgaa ggacgctgaa cccagcgcca gcgattcggt gtggacaatg
    15361 ttttgcgccg ggaagccgaa gcgttgcaga tattctgcag cgtagtcgac gcccatagcc
    15421 cgcattgcac gtaccatcac cacgtttttc gactgaccca gcccctgacg taagcgaatt
    15481 ggaccagcat attgtggcgg cgagtttttc ggctgccagt cagaaccggc acctgcgtcc
    15541 cagcgagaaa ttggcacatc gttcaacata cttgccagcg tcagaccttt atccatcgcc
    15601 gcggtgtaga ggaacggttt gatgttggaa cccacctgac gcagtgcctg ggtggcgcgg
    15661 ttaaacttgc tctgattgaa atcaaagcca ccgaccagcg ccataacggc accgttttgc
    15721 ggattgatcg acaccagcgc cgagttcact tccggcactt gtgccagcca ccatgcatcg
    15781 ccaacctgac gaacccagat ttgctgaccc gtttgcagaa catcggtcac tttacgcggc
    15841 gtcggtccct gctgagtatc cgaacggtaa ggacgcgccc agcgaacgcc ttccatactc
    15901 aatgcgacgg tcgacccgtc cgccagcatc gccgtcgctc cctgaggatt ggcgctggtg
    15961 actgcggcag gcagcagcgg accataggtc ggcagcgcct tcagcgtatc ggtaatcttg
    16021 ttgttatccc acgccgactc gcccactttc cacagcacat ttgccgggcc gcgatagccg
    16081 tggcgcatgt cgtagtccag cacgttatta cgtaccgcct gctgtgcggc ctgctgcact
    16141 ttgcgggtga tggtggtgta aatgcgataa ccgtcttcat aggcactttc gccataacgg
    16201 ttatacatct cctggcgcac catttcgctc aggtacggcg cagagaaagc aatctccggc
    16261 gcgtgatagt tagcgttaat cgcctcagtg cgtgtctgat cgaactgttg ttgggtgata
    16321 tacccttcat ccagcatccg cgacagtacg acgttacgcc gcgcgacggc acgatccatt
    16381 gagtagagcg gattaaaggt ggaaggcgct ttcggcagcc cggctatcac agccatttcg
    16441 ttcagcgtca gttggtcgac cgttttaccg aaatagactt gtgccgcagc accgacacca
    16501 taggcgcggt aaccgaggta aatcttattc agataaagct cgaggatctc gtctttcgtc
    16561 agcaactgtt caatgcgaat cgcgaggaag acttccttaa tcttacgcat cagcgtgcgt
    16621 tctggactga ggaagaagtt tctcgccagc tgctgggtaa tggtactcgc cccttgtgac
    16681 gcgtggccgg agaacagcgc cacacttgcc gcacggaaga tccccaccgg gtcaacgccg
    16741 tgatgctcgt agaagcggct gtcttccgtc gcgataaagg ctttcaccat ctccggcggg
    16801 atttgatcca acgtaaccgg aatacgacgt ttctcaccgt attgagcaat cagctcgcca
    16861 tcggcgctgt aaatctgcat cggaatttgc aggcgaacat cttttaatgt cgccacatcc
    16921 ggcagttgtg gctcgatgta gcggtatagg ccataaatcg agcctgctcc cagcagaatg
    16981 caacagactg caaggatcaa aaaatacttt acgaacttca ctggaaattt cccatttagt
    17041 ttcatttggg cagtttataa acaaacgcgc gttagtataa aggcaagcca gacgcattga
    17101 tatacccgtc agagtgacgg gtgataagga gatcatcaca atggcattta agatctggca
    17161 aattggtttg catttacaac agcaagaagc ggtagcggtt gcgatcgtgc ggggcgcaaa
    17221 agaatgcttt ttgcaacgct ggtggcggtt gccgctggag aacgacatta tcaaagatgg
    17281 gcggattgtt gatgcgcagc agctggctaa aacgttgtta ccttggagtc gcgaactgcc
    17341 gcagcgtcat cacattatgt tggcgtttcc cgccagtcgc acattacagc ggtcatttcc
    17401 gcgcccgtcg atgtcccttg gtgagcggga gcaaacggcc tggctgtcag ggacgatggc
    17461 ccgcgagctg gatatggatc cggactccct gcgcttcgat tacagcgaag actcactcag
    17521 tcccgcctat aacgtgactg ccgcgcaaag caaagagctg gcaacgctgc ttactctggc
    17581 agaaaggttg cgtgttcatg ttagtgcgat caccccggat gccagtgcat tacagcgatt
    17641 cctgcctttt ttaccttctc atcagcaatg tctggcctgg cgtgataacg aacagtggct
    17701 gtgggcgacg cgctatagct gggggcgcaa actggcggtg gggatgacta gcgcgaagga
    17761 gctggcggca gcgttattcg ttgatcccga cagcgtcgcg atatgtggcg aaggcggatt
    17821 tgacccctgg gaggccgttt ctgttcgtca gccgctgcta ccgccgcctg gtggagactt
    17881 tgccatcgcg ctggggctgg cgcttgggaa ggcgtactga tgaacccgcc aattaatttt
    17941 ttgccctggc gacagcaacg ccggaccgct tttctgcgtt tctggttgct gatgttcgtt
    18001 gcgcctctgc tgctggccgt cgggataacg ctaatactgc gtctgacagg cagcgccgaa
    18061 gcgcgcatag acgcagtttt gcttcaggcg gaacaacaac tcgcccgcag cttacagata
    18121 acgaagccac gtttgctgga gcaacagcag ttacgcaaac agcgttcgca acggcagcgc
    18181 cagcgacaat ttacccgcga ctggcaatct gcgctggaag cactggcggc gcttttaccc
    18241 gagcacgcct ggctgacaac gataagctgg cagcagggaa cgctggagat caaggggctg
    18301 acaacaagca ttaccgcgtt aaacgcacta gaaacgtcac tccgccagga tgcgtctttt
    18361 catctcaatc agcggggagc cacgcagcag gatgcgcagg gacgctggca atttgagtat
    18421 cagttaacaa ggaaggttag cgatgaacat gttctttgac tggtggttcg ccacatcacc
    18481 ccgcctccgc cagttttgct gggcattctg gttgctgatg ttagttacgc tcatttttct
    18541 gtcatcgaca caccatgaag agcgcgacgc attaattcga ttacgggcaa gtcatcacca
    18601 gcagtgggcc acactgtatc gcctggttga caccactccc ttcagcgaag aaaaaacgct
    18661 gcccttttcg ccactggatt ttcagttgcc cggcgcgcaa ctggtttcct ggcatccatc
    18721 cgcgcaggga ggtgagttgg cgttgaaaac gctgtgggaa gcagtgccgt cggcattcac
    18781 acggctggca gagcgcaacg tcagcgtgag ccgtttttcg ttaagcgtgg aaggtgatga
    18841 tcttttgttc acgctacaac tggagacgcc gcatgagggt taaacgctgg ttgttggcag
    18901 gtattgcatt gtgcctttta accggtatgc gtgacccttt taaaccgccg gaagatctat
    18961 gccggattag cgaacttagc cagtggcgct atcaggggat ggtagggcga ggcgagcgca
    19021 tcatcggtgt aataaaagac gggcaaaaga aatggcgacg ggtgcagcaa aacgatgtgc
    19081 tggaaaacgg ctggacaatt ttacagctga cgccagacgt actaacgctg ggtaccggga
    19141 caaactgcga acagccacaa tggttgtggc aacggcaagg agatacaaat gaagcaatgg
    19201 atagccgcac tactgttgat gctgataccc ggcgtacagg cggcaaagcc gcaaaaagtg
    19261 acgctgatgg tggatgacgt tccggtagct caggtgttgc aggcgctggc tgaacaggag
    19321 aagttgaacc tggtcgtgtc gccagacgtc agcggtacgg tgtcgttaca tctaacagat
    19381 gttccctgga agcaggcact acaaactgta gtgaaaagcg ccggactgat aacgcggcag
    19441 gaaggcaaca ttctctcagt gcattccatt gcctggcaga ataacaatat cgcccgccag
    19501 gaggcggagc aggcgcgggc gcaggcaaat ctgccgctgg aaaatcgcag tataaccctg
    19561 caatacgccg acgcgggaga actggcgaaa gcgggggaga agctactgag tgccaaaggg
    19621 agtatgaccg tcgataaacg caccaatcgc cttttgctac gagataacaa aacggcgtta
    19681 agcgcgcttg aacagtgggt agcgcaaatg gatctgccgg tcgggcaggt tgagctgtcg
    19741 gcgcatattg tcaccattaa tgaaaaaagt ttgcgtgagt taggcgtgaa atggacgctg
    19801 gccgatgcgc aacacgctgg tggcgttggg caagtcacca cgcttggtag cgacctctcc
    19861 gtagcgacgg cgacaacgca tgtcggtttt aacattgggc gcatcaacgg acgcttgctg
    19921 gatcttgagc tttccgcgct cgaacaaaaa cagcagctgg atattatcgc cagtccgcgt
    19981 ctgctggcct cacatcttca gcctgccagc attaaacagg ggagcgaaat tccatatcag
    20041 gtttccagcg gggaaagtgg cgcgacgtcg gtggaattta aagaggccgt cctggggata
    20101 gaggtcacgc ccacggtgtt acaaaaaggt cgcatccggc tgaaattaca catcagccag
    20161 aacgttccgg ggcaggtgct acagcaggcc gatggcgaag tgctggcgat tgataagcag
    20221 gagatcgaaa cgcaggtcga ggtcaaaagc ggagaaacgt tggcgctggg cggcattttt
    20281 acccgtaaaa ataaatcggg tcaggatagc gtaccgttgc ttggcgacat tccctggttc
    20341 gggcaattat ttcgtcatga cggaaaagaa tatgaacgac gcgagttagt ggtgtttatc
    20401 acgccacgac tggtttccag tgagtaaaca gccgtaaaag cggtaatgtt tttatgctga
    20461 acgtgtttca tctatttgac gcgcgcaggt atttagcata caaggagtac cgatttgaga
    20521 gttggcgctc ttcgctgcct gcgttccatg atgatgattt atcattcagg cggcattttg
    20581 ctgtcttttt tacgctaatc ttacccggtg atttatcgcc agagcggtgg tagcaaggca
    20641 gcgcgcttgc agcgaccaga tatgcagagg gatgggtgat ttattcagtt gccaaacccg
    20701 ctggagtatt gagataattt tcagtctgac tctcgcaata tcttatgagg tttcagttca
    20761 tgtcctgcgg cgctctctga gcgaagcggg tttatcatta acgaatagtc ttagtagtac
    20821 cgaaaaaatg gcagagaaac gcaatatctt tctggttggg cctatgggtg ccggaaaaag
    20881 cactattggg cgccagttag ctcaacaact caatatggaa ttttacgatt ccgatcaaga
    20941 gattgagaaa cgaaccggag ctgatgtggg ctgggttttc gatttagaag gcgaagaagg
    21001 cttccgcgat cgcgaagaaa aggtcatcaa tgagttgacc gagaaacagg gtattgtgct
    21061 ggctactggc ggcggctctg tgaaatcccg tgaaacgcgt aaccgtcttt ccgctcgtgg
    21121 cgttgtcgtt tatcttgaaa cgaccatcga aaagcaactt gcacgcacgc agcgtgataa
    21181 aaaacgcccg ttgctgcacg ttgaaacacc gccgcgtgaa gttctggaag cgttggccaa
    21241 tgaacgcaat ccgctgtatg aagagattgc cgacgtgacc attcgtactg atgatcaaag
    21301 cgctaaagtg gttgcaaacc agattattca catgctggaa agcaactaat tctggcttta
    21361 tatacactcg tctgcgggta cagtaattaa ggtggatgtc gcgttatgga gaggattgtc
    21421 gttactctcg gggaacgtag ttacccaatt accatcgcat ctggtttgtt taatgaacca
    21481 gcttcattct taccgctgaa atcgggcgag caggtcatgt tggtcaccaa cgaaaccctg
    21541 gctcctctgt atctcgataa ggtccgcggc gtacttgaac aggcgggtgt taacgtcgat
    21601 agcgttatcc tccctgacgg cgagcagtat aaaagcctgg ctgtactcga taccgtcttt
    21661 acggcgttgt tacaaaagcc gcatggtcgc gatactacgc tggtggcgct tggcggcggc
    21721 gtagtgggcg atctgaccgg cttcgcggcg gcgagttatc agcgcggtgt tcgtttcatt
    21781 caagtcccga cgacgttact gtcgcaggtc gattcctccg ttggcggcaa aactgcggtc
    21841 aaccatcccc tcggtaaaaa catgattggc gcgttctacc agcctgcttc agtggtggtg
    21901 gatctcgact gtctgaaaac gcttcccccg cgtgagttag cgtcggggct ggcagaagtc
    21961 atcaaatacg gcattattct tgacggtgcg ttttttaact ggctggaaga gaatctggat
    22021 gcgttgttgc gtctggacgg tccggcaatg gcgtactgta ttcgccgttg ttgtgaactg
    22081 aaggcagaag ttgtcgccgc cgacgagcgc gaaaccgggt tacgtgcttt actgaatctg
    22141 ggacacacct ttggtcatgc cattgaagct gaaatggggt atggcaattg gttacatggt
    22201 gaagcggtcg ctgcgggtat ggtgatggcg gcgcggacgt cggaacgtct cgggcagttt
    22261 agttctgccg aaacgcagcg tattataacc ctgctcaagc gggctgggtt accggtcaat
    22321 gggccgcgcg aaatgtccgc gcaggcgtat ttaccgcata tgctgcgtga caagaaagtc
    22381 cttgcgggag agatacgctt aattcttccg ttggcaattg gtaagagtga agttcgcagc
    22441 ggcgtttcgc acgagcttgt tcttaacgcc attgccgatt gtcaatcagc gtaacaacaa
    22501 gaaaggtcag gccgcttatc aagcggtcta ttagcttcag gttaattgca acgtggtaag
    22561 cattaacctt ttagtggggt gttaaatgga tgaattcaaa ccagaagacg agctgaaacc
    22621 cgatcccagc gatcgtcgta ctggtcgttc tcgtcaatct tctgaacgtt ctgagcgtac
    22681 tgaacgtggc gaaccgcaga tcaattttga tgatattgaa cttgatgaca ctgacgatcg
    22741 ccgtccgact cgtgcgcaaa aagagcgcaa tgaggaaccg gaaatcgaag aagaaattga
    22801 cgaatccgaa gatgaaaccg tggatgaaga gcgcgtagag cgtcgtccgc gtaagcgcaa
    22861 aaaagcagcc agtaaacccg cttctcgtca gtatatgatg atgggcgtcg gcattctggt
    22921 tctactgctg ttgatcatcg gtatcggttc tgcgctaaaa gccccctcga cctcttccag
    22981 cgatcaaacc gcgtctggcg ataagagtat tgatcttgca ggcaatgcga ccgatcaggc
    23041 gaatggggtg cagccagcgc cgggaaccac gtctgcggaa aatactcagc aggatgtttc
    23101 tctgccaccg atctcttcta cgccgactca agggcaaacc ccggcggcaa cggatggtca
    23161 acaacgtgtt gaagtgcagg gtgacctgaa caatgcgctg actcagccac aaaatcagca
    23221 acagttgaac aatgtggcgg tcaattccac attgccgacc gaacccgcga ctgtcgcgcc
    23281 tgttcgcaat ggcaatgcat cgcgtgacac ggcgaaaacg caaaccgctg aacgtccgtc
    23341 cactacgcgc ccagctcgtc agcaggcggt gattgaaccg aaaaaaccgc aagcaaccgt
    23401 gaaaacggag ccgaagccgg tagcacagac gccgaagcgt actgaaccag ctgcccctgt
    23461 ggcgagcacg aaggcaccgg ctgcgacttc tacgccagca ccaaaagaga cggcgactac
    23521 ggctccagta cagacggcat ccccggcgca aaccacggca acaccagccg ctggagggaa
    23581 gaccgcaggt aatgttggtt cgttgaaatc ggcaccgtcc agccattaca ctctgcagct
    23641 gagcagttcc tctaactacg acaacctgaa cggttgggcg aagaaagaga atctgaaaaa
    23701 ctacgttgtc tatgaaacga cgcgtaatgg tcagccgtgg tatgtcctgg tttctggcgt
    23761 gtacgcttcg aaagaagagg cgaaaaaagc ggtatctaca ttgccagcag atgttcaggc
    23821 caaaaacccg tgggcgaaac cgctgcgtca ggtacaggcc gatctgaagt aatcaaggtt
    23881 atctcccgca atggtttatc gttgcgggag ctgcctgaag cgctggatgc tgtcggagct
    23941 ttctccacag ccggagaagg tgtaattagt tagtcagcat gaagaaaaat cgcgcttttt
    24001 tgaagtgggc agggggcaag tatcccctgc ttgatgatat taaacggcat ttgcccaagg
    24061 gcgaatgtct ggttgagcct tttgtaggtg ccgggtcggt gtttctcaac accgactttt
    24121 ctcgttatat ccttgccgat atcaatagcg acctgatcag tctctataac attgtgaaga
    24181 tgcgtactga tgagtacgta caggccgcac gcgagctgtt tgttcccgaa acaaattgcg
    24241 ccgaggttta ctatcagttc cgcgaagagt tcaacaaaag ccaggatccg ttccgtcggg
    24301 cggtactgtt tttatatttg aaccgctacg gttacaacgg cctgtgtcgt tacaatctgc
    24361 gcggtgagtt taacgtgccg tttggccgct acaaaaaacc ctatttcccg gaagcagagt
    24421 tgtatcactt cgctgaaaaa gcgcagaatg cctttttcta ttgtgagtct tacgccgata
    24481 gcatggcgcg cgcagatgat gcatccgtcg tctattgcga tccgccttat gcaccgctgt
    24541 ctgcgaccgc caactttacg gcgtatcaca caaacagttt tacgcttgaa caacaagcgc
    24601 atctggcgga gatcgccgaa ggtctggttg agcgccatat tccagtgctg atctccaatc
    24661 acgatacgat gttaacgcgt gagtggtatc agcgcgcaaa attgcatgtc gtcaaagttc
    24721 gacgcagtat aagcagcaac ggcggcacac gtaaaaaggt ggacgaactg ctggctttgt
    24781 acaaaccagg agtcgtttca cccgcgaaaa aataattctc aaggagaagc ggatgaaaca
    24841 gtatttgatt gccccctcaa ttctgtcggc tgattttgcc cgcctgggtg aagataccgc
    24901 aaaagccctg gcagctggcg ctgatgtcgt gcattttgac gtcatggata accactatgt
    24961 tcccaatctg acgattgggc caatggtgct gaaatccttg cgtaactatg gcattaccgc
    25021 ccctatcgat gtacatctga tggtgaaacc cgtcgatcgc attgtgcctg atttcgctgc
    25081 cgctggtgcc agcatcatta cctttcatcc agaagcctcc gagcatgttg accgcacgct
    25141 gcaactgatt aaagaaaatg gctgtaaagc gggtctggta tttaacccgg cgacacctct
    25201 gagctatctg gattacgtga tggataagct ggatgtgatc ctgctgatgt ctgttaaccc
    25261 aggttttggc ggtcagtcgt tcattccaca aacactggat aaactgcgcg aagtacgtcg
    25321 tcgtatcgac gagtctggtt ttgacattcg actggaagtg gacggtggcg tgaaggtgaa
    25381 caacattggc gaaatcgctg cggcgggcgc ggatatgttc gtcgccggtt cggcaatctt
    25441 cgaccagcca gactacaaaa aagtcattga tgaaatgcgc agtgaactgg caaaggtaag
    25501 tcatgaataa gtttgaagat attcgcggcg tcgcttttga tctcgacggt acgctggttg
    25561 acagtgctcc cggtcttgct gctgcggtag atatggcgct gtatgcgctg gagttacccg
    25621 tcgcaggtga agaacgcgtt attacctgga ttggtaacgg cgcagatgtt ctgatggagc
    25681 gtgcattggc ctgggcgcgt caggaacgtg cgactctgcg taaaacaatg ggtaaaccgc
    25741 ccgttgatga cgacattccg gctgaagaac aggtacgtat tctgcgtaaa ctgttcgatc
    25801 gctactatag cgaggttgcc gaagagggga cgtttttgtt cccgcacgtt gccgatacgt
    25861 tgggcgcgtt gcaggctaaa ggcctgccgc taggcctggt caccaacaaa ccgacgccgt
    25921 tcgtcgcgcc actgctcgaa gccttagata ttgccaaata ctttagcgtg gttatcggcg
    25981 gcgatgatgt gcaaaacaaa aaaccgcatc cggacccgct gttactggtg gctgagcgga
    26041 tgggaattgc cccacaacag atgctgtttg tcggcgactc acgcaatgat attcaggcgg
    26101 caaaagcggc aggttgccca tcagttggct taacctacgg atataactac ggcgaggcta
    26161 tcgatctcag ccagcctgat gtaatttatc agtctataaa tgaccttctg cccgcattag
    26221 ggcttccgca tagcgaaaat caggaatcga aaaatgacta agcccatcgt ttttagtggc
    26281 gcacagccct caggtgaatt gaccattggt aactacatgg gtgcgctgcg tcagtgggta
    26341 aacatgcagg atgactacca ttgcatttac tgtatcgttg accaacacgc gatcaccgtg
    26401 cgccaggatg cacagaagct gcgtaaagcg acgctggata cgctggcctt gtatctggct
    26461 tgtggtatcg atcctgagaa aagcaccatt tttgttcagt cccacgtacc agaacatgcg
    26521 cagttaggct gggcactgaa ctgctatacc tacttcggcg aactgagccg catgacccag
    26581 tttaaagata aatctgcgcg ttatgccgag aacatcaacg ctggtctgtt tgactatccg
    26641 gtgctgatgg ctgcggacat cctgctgtat caaactaatc tggtaccggt gggtgaagac
    26701 cagaaacagc acctggaact gagtcgcgat atcgcccagc gtttcaacgc gctgtatggc
    26761 gagatcttta aggtgccgga gccgtttatt ccgaaatctg gcgcgcgcgt aatgtcgctg
    26821 ctggagccga ccaagaagat gtccaagtct gacgataacc gcaataacgt tatcggcctg
    26881 ctggaagatc cgaaatcggt agtgaagaaa atcaaacgcg cggtcactga ctccgacgag
    26941 ccgccggtag ttcgctacga tgtgcagaac aaagcgggcg tttccaacct gctggatatc
    27001 ctttctgcgg taacgggcca gagcatccca gaactggaaa aacagttcga aggcaagatg
    27061 tatggccatc tgaaaggtga agtggctgat gccgtttccg gtatgctgac tgaattgcag
    27121 gaacgctatc accgtttccg caacgatgaa gccttcctgc aacaggtgat gaaagacggc
    27181 gcagaaaaag ccagtgtaca cgcttcccgt acgctgaaag cggtgtacga agcgattggt
    27241 tttgtggcga agccgtaata tcaaaacgct cggacggtcc cctcgcccct ttggggagag
    27301 ggttagggtg aggggagcag gcaagcgcca gtgggaatta ccctttgctc gcattgtcga
    27361 ataaatcact tcgtcatcac tctgaaaccg gggaaacccg gtttttttat cctcatttgc
    27421 aataatccga aaaaatgtga agcgcctcgc cgtttccaca tctcacagtt gccacttatc
    27481 ttcattttaa tgaagatata aacattcatt ttattgaaaa tatattatgc gtcgaacgtt
    27541 tatcaaaaaa gaaggcgtcg tcatcacgac gctggcccgt tatttgttgg gtgaaaaatg
    27601 cggtaatcga ctgaaaacca tagatgagct ggcaactgaa tgccgttcat cggttggcct
    27661 gacgcaggcc gcgttgaaaa cgttggaatc aagtggagcg atacggattg aacgccgtgg
    27721 gcgcaatggc agttatctgg tcgaaatgga taacaaagcg ctgctgagtc atgtggatat
    27781 caataacgtt gtgtgtgcca tgcctttgcc ctatacccgc ttgtacgaag gtttagcaag
    27841 cgggctgaaa gcccagtttg atggcattcc tttttattat gcgcatatgc gtggcgcgga
    27901 tattcgtgtg gagtgtttgc ttaacggtgt ctatgacatg gcagtagttt cccgcctggc
    27961 ggcggaaagt tatctcagcc aaaacaattt gtgcatcgcc ctcgagcttg gcccgcatac
    28021 gtatgttggt gaacatcaac tgatatgccg caaaggtgag tctggaaatg ttaagcgagt
    28081 aggtctcgat agtcgttcgg cggatcagaa aatcatgacc gatgtctttt ttggcgatag
    28141 tgatgtggaa cgtgtcgatc tctcttatca cgagagttta caacgcattg ttaaaggcga
    28201 tgtcgatgcg gttatctgga acgtggtggc ggaaaacgaa ctgaccatgc tgggattaga
    28261 ggcgacgccg ctcacagacg atccgcgatt tttacaggcc accgaagccg tggtcctgac
    28321 gcgagtcgat gattacccaa tgcaacaact gctgcgtgcc gttgtagata aacacgccct
    28381 gctcgcccat caacaacggg tagtgagtgg ggaacaggaa ccgagttatt aaacgaaagg
    28441 catctgatat ggaaaccaga ctcaacctgc tttgcgaggc aggcgttatt gataaggaca
    28501 tctgcaaagg catgatgcag gtcgtcaacg tactggaaac agagtgccat ctgccggtgc
    28561 gcagtgagca aggaacgatg gcgatgacac atatggcgag tgcactgatg cgcagtcgcc
    28621 gtggtgaaga aatagagccg ctggataacg aattgctggc agagctggcg caatccagcc
    28681 actggcaagc cgttgtgcaa ttgcatcagg tgttgttgaa ggaattcgca ctggaagtta
    28741 atccgtgtga agaaggctat ttgctggcca acctttatgg attatggatg gctgctaacg
    28801 aagaggtttg aagctcccga caaatccacg tcgcacaacc taaatattca aaaaaatgaa
    28861 tatttaattc aaggaacaga aacgaggcaa caagatgttt gtagaagcat tgaaacgcca
    28921 gaacccggcg ctgatttccg ccgcactaag tttgtggcag cagggcaaga tcgccccgga
    28981 cagctgggtg atcgacgtgg atcaggtact ggaaaacggt aagcgactga ttgagacggc
    29041 gcggctttac ggcattgaac tgtatctgat gaccaagcag tttggccgta acccatggct
    29101 ggcggaaaaa ttgctggcat taggttacag cggcattgtg gcggtggatt acaaagaggc
    29161 gcgagtcatg cgccgcgctg gtttgtcggt ggcgcatcag gggcatctgg tacaaatccc
    29221 ttgtcatcag gttgctgacg ccgttgaaca gggcaccgac gtcatcaccg tgtttactct
    29281 cgacaaagcg cgggaagttt ctgcggcagc ggtgaaggcc ggacgtgtgc agtttgtgct
    29341 gctcaaagtt tatagcgacg atgattttct ttatccgggg caggagagcg gttttgtaca
    29401 gcattcgctg cacgaggtgg ttgctgaaat taagaaactg ccgggtctgc atttagccgg
    29461 acttacccat ttcccttgcc tgctttggga tgaggctgcc ggaaaagttt tgccgacacc
    29521 gaatcttcac acgctgatac aggcacggga tcaactggcg aaatctggta ttgcacttga
    29581 gcaactgaac gcgccttcag cgaccagctg cacttcgctg ccattactgg cgcaatacgg
    29641 tgtgactcat gccgaacccg gtcatgcgct gacgggcact attccggcaa accagcaggg
    29701 cgatcagtct gaacgtatcg cgatgctctg gttgagtgaa atctcccatc atttccgtgg
    29761 cgacagctac tgctacggcg gcggttacta ccgtcgtggt catgcgcaac atgcgttggt
    29821 gtttacgcca gaaaatcaaa agattactga aaccaatctc aaaactgtgg atgacagcag
    29881 tatcgactac accctgtcgc tggcaggcga gtttccggta agcagtgcag tggtgctctg
    29941 tttccgcacg cagatttttg tcacccgtag cgatgtggtg ctggtgtccg gtattcaccg
    30001 tggcgaaccg aaaatcgttg gtcgttatga cagtcttgga aactctctgg gggcgtaatg
    30061 gcgcgatttg tggtgttagt gattgatagc tttggcgtag gggcaatgaa agatgtcacg
    30121 ctggtgcgtc cgcaagatgc gggagcgaat acatgtggtc acatcctgag ccagttgccg
    30181 catttgcagc taccaacgct ggagaagctg gggctaatca acgcattggg ttatgcgcca
    30241 ggcgatatgc agccgtcaga ttccgcaacc tggggcgtgg cagagctgca acatgaaggt
    30301 ggcgatacct ttatggggca tcaggaaatt ttaggcacgc gcccgttacc gccgctgcgg
    30361 atgccttttt gcgatgtgat tgaccgtgtt gagcaggcat tagtatccgc cggttggcag
    30421 gtggagcgcc gtggcgatga actgcaattt ctgtgggtca atcaggcggt tgcgattggc
    30481 gataatctcg aggcggattt aggccaagtc tataacatta ccgccaatct ctctgtgatc
    30541 tcttttgacg acgcaatcaa aattggtcgt atcgtgcgtg agcaggtaca ggtcggtcgg
    30601 gtcattacat ttggtggcct gttaaccgac agtcaacgca ttctcgatgc cgcagaaagc
    30661 aaagaagggc gctttattgg tatcaatgcg ccgcgttctg gcgcttatga caacggcttc
    30721 caggtcgtgc atatgggcta cggtgtcgat aaaaaagtgc aggtgccgca aaaactgtat
    30781 gaggcgggcg tgccaaccgt gctggtgggt aaggtggcag atatcgtcag caatccttat
    30841 ggcgtgagct ggcaaaacct ggtggatagc cagcggatta tggatatcac cctcgacgaa
    30901 tttaacaccc atccgacggc gtttatttgc accaatatcc aggaaaccga cctcgctggt
    30961 catgcagaag acgtcgcacg ttatgccgaa cgtttgcagg tcgttgaccg taaccttgcc
    31021 cggcttgttg atgcgatgca gccagatgat tgcctggtcg tgatggcgga tcacggcaac
    31081 gatccgacca ttggtcacag ccaccatacc cgcgaagtgg tgccgctgct ggtttatcag
    31141 caaggcctgg ctgctacgca gctcggtgtg cgcaccacgc tttctgatgt gggagctacc
    31201 gtgtgtgaat ttttccgtgc gccaccgcca caaaatggtc gctcttttct ttcctccctc
    31261 cggtttgcag gagacaccct atgagttttg atccgacggg ttacaccctg gcccatgagc
    31321 atctgcatat tgatctctcc ggctttaaaa acaacgtgga ctgccgcctt gatcagtatg
    31381 cgttcatttg ccaggagatg aacgacctga tgacccgggg tgtacgtaat gtgattgaga
    31441 tgaccaaccg ttacatgggg cgcaatgcgc aatttatgct cggtgtaatg cgcgaaaccg
    31501 ggatcaacgt ggtggcctgt actggttatt accaggatgc gtttttcccg gaacatgtgg
    31561 tgacccgcag cgtgcaggaa ctggcgcagg agatggtcga tgaaattgaa cagggtatcg
    31621 atggcacgga gctgaaagcc gggctcatcg cggagatcgg ctccagcgaa ggaaagatta
    31681 cgccgctgga agagaaggta tttattgccg ctgcgctggc gcataaccag accggacgcc
    31741 cgatctccac gcatacgtcg ttcagcacca tggggctgga acaactggct ctgttgcaag
    31801 cccacggagt tgatctttcg cgcgtcaccg ttggtcactg cgatctgaaa gacaacctcg
    31861 acaacatttt gaagatgatc gatctcggcg cgtacgtgca gttcgacacc attggtaaga
    31921 acagttacta cccggacgaa aagcgcattg cgatgctcca cgcgctacgt gaccgtgggt
    31981 tgctgaaccg cgtcatgctg tcgatggata ttacgcgccg ctcccattta aaagccaatg
    32041 gtggttatgg ctatgactat ttattgacca cctttattcc gcaattgcgc cagtcaggat
    32101 tcagtcaggc cgatgtggat gtgatgttac gtgaaactcc ctctcaattt ttccaataag
    32161 gacagactca tgaaaaagat tggcgttgca ggcttacagc gtgagcagat taaaaaaacc
    32221 attgaagcga cggctcctgg cagttttgaa gttttcattc acaacgacat ggaagcggca
    32281 atgaaagtga aatccgggca actggattac tacatcggtg cgtgtaatac cggtgcgggc
    32341 gcggcattgt cgattgccat cgcggtgatt ggctataaca aaagttgcac cattgccaaa
    32401 ccaggcatta aagcgaaaga cgagcatatc gccaaaatga tcgctgaagg aaaagtggcg
    32461 tttggccttt ccgttgagca cgtcgaacac gcgattccga tgctgattaa ccatctgaaa
    32521 taaaaggcac gactatggat ctgtatattc agattatcgt ggtggcgtgc ctgacgggta
    32581 tgacatcgct tctggcgcat cgctcggcgg ctgtttttca tgacggcatc cgcccgatcc
    32641 tgccgcaact gattgaaggc tatatgaacc gtcgcgaggc ggggagtatc gcttttggtc
    32701 tgagcattgg ttttgtggcc tcggtgggga tctcttttac cctgaaaacc gggctgctca
    32761 acgcatggtt actctttctt cctaccgata tcctcggcgt actggcgata aacagcctga
    32821 tggcgtttgg tcttggcgct atctggggcg tgttgatcct tacttgcctg ttgccagtaa
    32881 accagctgct gaccgcgctg ccggtggatg tattaggtag cctcggggaa ttaagctcgc
    32941 cggtagtttc tgcttttgca ctgttcccgc tggtggcgat tttctaccag tttggctgga
    33001 agcaaagtct ggtcgccgcc gtggttgttc tgatgacccg tgtggtagtc gtgcgctatt
    33061 tcccacatct taaccctgaa tccatcgaaa tctttattgg catggtgatg ctgctgggga
    33121 tcgcgataac tcacgacctg cgtcatcgtg atgaaaatga tattgatgcc agcgggcttt
    33181 cggtgtttga agaacgcacg tcgcggatta tcaaaaactt accctatatc gccatcgtgg
    33241 gagcattgat tgccgccgtt gccagcatga agattttcgc cggcagtgaa gtgtcgatct
    33301 tcactctgga gaaagcgtac tccgcaggcg taacgccgga acaatcgcaa acgctgatca
    33361 accaggcggc actggcagaa tttatgcgcg ggctgggttt tgtaccgttg attgccacca
    33421 ccgccttagc aacgggtgtg tatgcagttg cgggctttac ctttgtttat gcggtgggct
    33481 atctctcgcc gaatccgatg gttgcagcgg tattaggcgc agtggttatt tcggcggaag
    33541 tcttgctgct tcgttctatc ggcaaatggc tgggacgcta cccgtcggtg cgtaatgcgt
    33601 cggataacat ccgtaacgcc atgaatatgc tgatggaagt ggcgctgctg gtcggttcga
    33661 ttttcgcagc aattaagatg gcgggttata ccggattctc tatcgcggtt gccatttact
    33721 tcctcaacga atccctgggc cgtccggtac agaaaatggc ggcaccggtc gtggcagtaa
    33781 tgatcaccgg tattctgctg aatgttcttt actggcttgg cctgttcgtt ccggcttaag
    33841 gaaggcacct atgaagacgt ttcctctgca aagcctgacg cttattgagg cgcagcaaaa
    33901 gcagtttgcg ctggtggaca cgatttgccg tcactttccc ggcgcggagt ttctaaccag
    33961 cggtgatttg ggcttaacgc cggggctgaa tcaaccgcgt attacccaac gggtggagca
    34021 ggtgctggct gatgcatttc acgcacaggc tgcggcgctg gtgcagggcg cggggactgg
    34081 cgcgattcgc gccgggctgg cagctttgct caaaccgggg cagcgtcttc tggtgcatga
    34141 cgcgcctgtt tacccgacga cacgggttat tattgagcag atggggctga cgcttattac
    34201 tgttgatttc aatgacctgt cggcactgaa gcaggtcgtc gacgagcaac aaccggatgc
    34261 ggcgctggtg cagcatacgc gccagcagcc gcaggacagc tacgtgctgg cagatgtgct
    34321 ggcaacgttg cgcgcggcag gtgttccagc gttaaccgat gacaactatg cggtgatgaa
    34381 ggtggcgcga atcggctgtg aatgcggcgc gaatgtctcg acattttcct gcttcaagct
    34441 atttgggcca gagggtgttg gtgcagtggt cggcgatgct gatgttatca accgtattcg
    34501 cgccacgctt tactccggcg gtagccagat ccagggcgca caggcgctgg aagtattgcg
    34561 ttgtctggtg tttgcgccag tgatgcacgc ggtgcaggca ggggtatctg aacggttgct
    34621 ggctttgctt aacggtggtg aggtgccgga agtgaaaagc gcggtgattg ctaatgcgca
    34681 gtcgaaggtg ttgattgtcg agtttcatca gccgattgcc gccagagtgc tggaagaggc
    34741 gcaaaagcgc ggtgccttgc cttacccggt gggtgcagag tcgaaatatg aaatcccgcc
    34801 gctcttttat cgcctttccg gaacgtttcg ccaggcgaat ccacaatcag aacattgtgc
    34861 gattcgcatt aacccgaatc gcagcggtga agagacggtg ctgcggattt tgcgtgagag
    34921 tattgccagt atttaatgct ggatagcacc gtcaagctaa attccgtact gaacggtccc
    34981 ctcgcccctt tggggagagg gttagggtga ggggaacgta cgggatgtaa aattcaacgc
    35041 gccagcatcg ccggatgcga tgctggcgca tcttatccgg cctacggtta atgtctttta
    35101 ttatcaatag ttaaggtgat gcgattggcg cggcagaaga gttcggagac gtgcaccggt
    35161 ttgtcattct gatcaaaggc gattttgctg atgcgaaaca acggttcgcc cagttcgcat
    35221 ttcagccatt ttgcctgcgg gcgggtggcg gcaaagatat cgatggtctt tttgtcgctg
    35281 accactcgcg tgtcaaaacg ctcctgaaat aactgatagg tggagcttcc ttcgacgtaa
    35341 atctcatcaa agtcaggata acgcgacagc gggatccatg aactatcaat aaacagcggc
    35401 tctttatcga gatacatcac ccggcagaga tggaacactt cgctgtttcc ggggatgttc
    35461 agctttttgc aaaacggcgc ggcgctgacc cgttcctgtt cgatcacttt ctctttcgtc
    35521 gccttgcctt gtgagacgcc aaaatcggta aaaccactga cagtaagcag ggcgttttca
    35581 actttctggc tttgtacaaa ggtgcctttt ccctgccagc ggatcagtac gccgtccgcc
    35641 actaagtcgc tgatggcttt gcgaatggta atgcggctga cgttatattg tgtacaaagc
    35701 tcgttttcgg tagggatctg ttgcccggcc tggtaaaccc cctgcgcgat atcatccagc
    35761 agtcgctggc ggacggtagc gtagaggagt tgatgagagt agcggtccgt agctgacata
    35821 atcgaccctg aaataaagaa tgaattactc ggattatagg gtcgttttcc ggcaagtaac
    35881 agcacgttgg cgtgaagatg ctcacgccaa cgttatacct accaggcacc gtggtactga
    35941 atggttttcg ccgcgcacgc cgttccctgc gccatcgcct gcggtaatgt catccccgca
    36001 gaccagccgc aaaggaatcc ggcaatgaac gaatctccgg cacccatggt gtcgataacc
    36061 gtcaccggtt caggagcctg acgccagaac tgcgcgccat cccaggcaat gctgccgttt
    36121 tcacccagcg tgacaatcac tgttcctgcg ccacgggcaa caatcgcttt catcttcaga
    36181 cgcagcgttt cgtcttcttg cggtgcggag gcaaaggcaa aatcgagatg cggcaccagt
    36241 gtctgccaga gcgggctgtc ccacttgtcg gagaagtcga aagcggtaag tttgcccgca
    36301 gcgtgcagct gtgggaatgc gtcttccgca tgtccccaga ttgccgcgtg cacaatgtca
    36361 tactgcgcca gccaggcgta atcctcttca ctcagggcaa agtcggccat cacgccttcg
    36421 gtgtagtcgc caaaaacgcg atcattgtcg tgcagttcca cctgagtttg tgcggtaacg
    36481 ccgtgtttcg tatggacatg gctgatatcg acgcccatgc gggcgagatc ctgcttcagc
    36541 tttgtgccgt agtcatcgtc acccacccag gtaatgcatc ccggctgtat gccgtagcga
    36601 gtgcagtaca ccgccacatt gaccgcatta ccgccagaaa acgctttatt cagttgcggg
    36661 tagatatcga cgcagttatc gccgattgtc gccagggttt tcatctctca tcctccggca
    36721 gcagcgcgcg aaagcgttcc agcgcctggc gggcatagag tctgggctcg ttcatataca
    36781 tcgtcaccag ctccaccgta cagtaaccct catagccccg ctcaataata tcgcgcatca
    36841 gttcccgcag cggcattttg ccttctccag gaatgtaatg cgtgtcgctg gccccgtcgc
    36901 tgtcgacaat atgcagatga cgtaatttat cgcccagttt gtcgaaataa ctcatcaccg
    36961 gttccgcctg gacatacggc gcgcaaatgt cgaccatgct gaacaggcgc ggcgaaggca
    37021 ccagcgccag cgcatgaagc acatcattag cattacacac gatgttcgat tcatacggcg
    37081 ttaacggttc gaggattaga tccatgccga tgttttccgc gtactcatac agttcgctta
    37141 ggttctctgc cagccgtccc cagataacat taggtggcgt gagatagccc gcgtgggccg
    37201 cggaaatcag cgtataaccc gcgttcatct cttttgccat atccatcgcc agcttgatca
    37261 tgtcgaggct ttcgcgacgc atatgttcat cgcccagcat catgttatac ggatagccat
    37321 tggtttctgg cgtatagccg ataatcggca tctgatacgt ctgcgccagc gccttgattt
    37381 gtttgatgcc gcccgctttt aagtccggcg cgaacgcgtg cgggcgaccg ccccaaattt
    37441 cgatgccgtc gtaacccagc tcgcttgcat cacgaaatgc gtgttcaata ggcagccgct
    37501 ggtggccgca ggtaaacata cctgttttta tcgggtaact ccttgcgtaa atccggcccg
    37561 ttgccgggcc gggcgaggga ttaatattcc accagaccac cgtaatagcg gcgttcatcc
    37621 gggttgtgat ctttgtaaat agacaggtag tagcagagcc actccattgg cacgaacatc
    37681 aggaacggtg ccagccacgg gtgcagccct tgcgaaattt cggcgtaatc gatgacgatc
    37741 acgttgtcag tacgctgttt aacaaagtta atggcgcgtt cggtggtgtg gcgactttca
    37801 tcattgccga gcaggaacag gaacggaacg cccggttcga caatctccag cgggccatgg
    37861 cggaactctc cgctctcaat cacgcagccg tgcgtccagg taaattccat cagcgttaca
    37921 atgccttctt tgtaacccag cggacgcagc ggacccgcag caacggtata aatcatcggc
    37981 cactggctgg ccagttcacc aagctggcgg cctttttctt cccaggtgcg taccagatga
    38041 ccgagcgcat tcggcaactg cttgagatcg tttttgatct tgccgatttc cgcgttcggc
    38101 gcgaggcggg tgatcatctc cagcaccacg ctgtagcaga gcagcaggtg aatttcccag
    38161 atacagtcgg cctgataatc aatgctaaat tccgccgccg aggtaatcgg gctgtccgcg
    38221 cgtttggtga acgccgcagt gagtgcgccg caggcccggc ccagctccag cgctttgatt
    38281 acctcttccg ttttaccgta gtcagaaacg ccaatcaccg cgcaacgatc gtcgaggcga
    38341 tacggggtgt tatcgcagaa ctcccagccg gaaatggcgt agacctgaag atcggaaaag
    38401 cgatccgcca gatgtttcgc cgcttgcgcc gcgttgagtg gcgagccgca ggcaacgaaa
    38461 taaatacgat caatgtcgcg cttcaccatc tcttccacga tggcgtgcac cagcggaacg
    38521 tcatggctga gaactttttc cacttcctga accatatttt cggtgaccag aaagtccacg
    38581 gtgcttttat caatatccaa cattttattt tctccagata acattacgac aaagcgttga
    38641 gctggcggct acgcttcgcc cagaaggcgt aagcaggcag accagtagca ataacgatga
    38701 cggcgcagat aaggccggga atcggtgccc agacaaaggt tgaggcgacc agaatgaggc
    38761 ttgacgcaat ggcgagggtg gtcatcagcc cgaaagcagg agtacgccac agcggtttgt
    38821 aatcgtcgcg tttacgacac cagatgatgg agccgaaggt gagggtattt ttgaaacaca
    38881 tcaccagggt gaaataaccc agcaggctgg tgagatcgga aacgaagatg aagaagagcc
    38941 ccagcgcccc ttgcaggatg atggagacat ccggcgtgtt gtatttcgga tgcacatggc
    39001 cgaagcattt aaagaacagg ttgtctttcg ccatcgcgta ttccagccgc ggctggtaca
    39061 tcacgcagct ggaaagcgaa ccaagaatga cgatcatcgc cgtgatggca acaaagatcc
    39121 cagcggtgct gccgagtgcg gggatccagg tcagggcgtc ggaaatcggc gtttcagaat
    39181 tggcgagttt gtcgaagggc atcaggtcgg aaatcaccag cgccagcagg gtgtagagca
    39241 ccagaaccag cagacaggaa ccaatcagcg ctcgtggcat ggtttttccg gggtttttaa
    39301 tttcgccggt catataacag atagaggcca tgccggtata cgaccaactg gtggcagaga
    39361 tccccgccag cagcgccata aagctgcccg ttgcgccaat cgcagtggtg gtaggggcgg
    39421 caaaattctc cgctttaaac cagaagatcc caaggccaat gacgatagtg aacgggataa
    39481 ttttggcgat ggtaattagc gtctgaaacg ctgcgccgcc ttcaacggag cgcaggtgta
    39541 gcaacataaa ggcgataatt aatccggcgg cgataaattt accgggcaac ggatcgatag
    39601 gcgttaaaaa gccaagattg ctgacaatcg ccagcgccat aatcgacaat gacggcgcat
    39661 cgttggccca gaagctggcc cagccggaga ggaaagccag cggtcggctt ccggcatttt
    39721 tcagataaac ataatctgcg ccattttccg gataagcggt ggatagttcc gcatagacgc
    39781 acatttgcgg gatcacaatt aacccgccaa tgacaaacgt gagcaccgta agccacggcg
    39841 tgcccgctgc ttttgccact tcacccacag atacaaaaat accggagccg acggttgtcc
    39901 cgacggcgat tgcaagaacg gcccaaaatc cgagcttgcg ttggagttcc tggcttccca
    39961 tagcgatacc tttttcttgt ccaacacgtt gtaggggaga gtttgcagtc atttaaaagg
    40021 taacatgaca atgcatgatg aatataataa catatgatgt tatgtaatgc ggaggaagat
    40081 cacaaaattt cgcacaggat cgcgctgtgg ctaatgaatg tagttatcca attgaaatta
    40141 aagtgaaaat atttttacgg gtggggagca agaaggatat agaaacaaat acgccctcgg
    40201 aaaatccaga gggcgtcggg caattaaacc ggtgttagcc gatttctgtc agagacttac
    40261 tgtgcagtag gaccgcaacc gccaatgatt ttagaaatag aaattgccgg gtgcagcagg
    40321 tagtcgtaag agcagttctt atcacggttt tcaatatgac cagtacatgc agttaacgta
    40381 gccagtactg cacctaccag tgcaacttta ataaattt
//
LOCUS       DAJTOR010000013        40059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTOR010000013 DAJTOR010000000
VERSION     DAJTOR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.013"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(458..2149)
                     /gene="fruA"
                     /locus_tag="OO249_000642"
     CDS             complement(458..2149)
                     /gene="fruA"
                     /locus_tag="OO249_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5635046.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAVARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2166..3104)
                     /gene="fruK"
                     /locus_tag="OO249_000643"
     CDS             complement(2166..3104)
                     /gene="fruK"
                     /locus_tag="OO249_000643"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5635047.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(3104..4234)
                     /gene="fruB"
                     /locus_tag="OO249_000644"
     CDS             complement(3104..4234)
                     /gene="fruB"
                     /locus_tag="OO249_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5635048.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4602..5783
                     /gene="setB"
                     /locus_tag="OO249_000645"
     CDS             4602..5783
                     /gene="setB"
                     /locus_tag="OO249_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5635049.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRTKD
                     V"
     gene            complement(5780..6034)
                     /locus_tag="OO249_000646"
     CDS             complement(5780..6034)
                     /locus_tag="OO249_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5635050.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            6189..6761
                     /gene="yeiP"
                     /locus_tag="OO249_000647"
     CDS             6189..6761
                     /gene="yeiP"
                     /locus_tag="OO249_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5635051.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6984..8450
                     /locus_tag="OO249_000648"
     CDS             6984..8450
                     /locus_tag="OO249_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5635052.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMNGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8568..9554
                     /gene="yeiR"
                     /locus_tag="OO249_000649"
     CDS             8568..9554
                     /gene="yeiR"
                     /locus_tag="OO249_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5635053.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWTVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9593..10306
                     /gene="lpxT"
                     /locus_tag="OO249_000650"
     CDS             9593..10306
                     /gene="lpxT"
                     /locus_tag="OO249_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5635054.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10718..11284
                     /gene="mepS"
                     /locus_tag="OO249_000651"
     CDS             10718..11284
                     /gene="mepS"
                     /locus_tag="OO249_000651"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5635055.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11465..13021
                     /locus_tag="OO249_000652"
     CDS             11465..13021
                     /locus_tag="OO249_000652"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5635056.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGGETPEQARWLSEPGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            13103..14917
                     /locus_tag="OO249_000653"
     CDS             13103..14917
                     /locus_tag="OO249_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5635057.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQQLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14918..16012
                     /locus_tag="OO249_000654"
     CDS             14918..16012
                     /locus_tag="OO249_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5635058.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            16012..17037
                     /locus_tag="OO249_000655"
     CDS             16012..17037
                     /locus_tag="OO249_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5635059.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            17039..18628
                     /gene="yejF"
                     /locus_tag="OO249_000656"
     CDS             17039..18628
                     /gene="yejF"
                     /locus_tag="OO249_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5635060.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKLQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18632..18976)
                     /locus_tag="OO249_000657"
     CDS             complement(18632..18976)
                     /locus_tag="OO249_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635061.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19309..20492)
                     /gene="bcr"
                     /locus_tag="OO249_000658"
                     /pseudo
     CDS             complement(19309..20492)
                     /gene="bcr"
                     /locus_tag="OO249_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20520..21215)
                     /gene="rsuA"
                     /locus_tag="OO249_000659"
     CDS             complement(20520..21215)
                     /gene="rsuA"
                     /locus_tag="OO249_000659"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5635062.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21364..23124
                     /locus_tag="OO249_000660"
     CDS             21364..23124
                     /locus_tag="OO249_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5635063.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23249..23533
                     /gene="rplY"
                     /locus_tag="OO249_000661"
     CDS             23249..23533
                     /gene="rplY"
                     /locus_tag="OO249_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5635064.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23672..24679)
                     /gene="yejK"
                     /locus_tag="OO249_000662"
     CDS             complement(23672..24679)
                     /gene="yejK"
                     /locus_tag="OO249_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5635065.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24861..25088
                     /locus_tag="OO249_000663"
     CDS             24861..25088
                     /locus_tag="OO249_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5635066.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            25108..26868
                     /gene="pbgA"
                     /locus_tag="OO249_000664"
     CDS             25108..26868
                     /gene="pbgA"
                     /locus_tag="OO249_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5635067.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRPQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26943..27019
                     /locus_tag="OO249_000665"
     tRNA            26943..27019
                     /locus_tag="OO249_000665"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26977..26979,aa:Pro,seq:ggg)
     gene            27238..28479
                     /locus_tag="OO249_000666"
     CDS             27238..28479
                     /locus_tag="OO249_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5635068.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            28975..29181
                     /locus_tag="OO249_000667"
     CDS             28975..29181
                     /locus_tag="OO249_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR5635069.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLAWQKEREQEARGTAA"
     gene            29196..29861
                     /locus_tag="OO249_000668"
     CDS             29196..29861
                     /locus_tag="OO249_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635070.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVYDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSV
                     IVLSCIAMRRNTSHHGADSFYPLGSMPRRFSSVRPNSLSQLARLMPSLCASLSNCSFS
                     SGEIRILNCGDCPSPLGLLSRLIIDKWSPIELAFPLLGGHLNTRALKKAKPRKCHYHS
                     QGF"
     gene            29887..30087
                     /locus_tag="OO249_000669"
     CDS             29887..30087
                     /locus_tag="OO249_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5635071.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            30077..30298
                     /locus_tag="OO249_000670"
     CDS             30077..30298
                     /locus_tag="OO249_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635072.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            30291..30524
                     /locus_tag="OO249_000671"
     CDS             30291..30524
                     /locus_tag="OO249_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635073.1"
                     /translation="MRDDRFNSLKQEFPGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            30517..30750
                     /locus_tag="OO249_000672"
     CDS             30517..30750
                     /locus_tag="OO249_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635074.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            30756..31055
                     /locus_tag="OO249_000673"
     CDS             30756..31055
                     /locus_tag="OO249_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5635075.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            31052..32452
                     /locus_tag="OO249_000674"
     CDS             31052..32452
                     /locus_tag="OO249_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5635076.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPVEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVISIDGDDVAPL"
     gene            32652..32903
                     /locus_tag="OO249_000675"
     CDS             32652..32903
                     /locus_tag="OO249_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635077.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            32900..33310
                     /locus_tag="OO249_000676"
     CDS             32900..33310
                     /locus_tag="OO249_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR5635078.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            33321..33593
                     /locus_tag="OO249_000677"
     CDS             33321..33593
                     /locus_tag="OO249_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5635079.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            33832..34038
                     /locus_tag="OO249_000678"
     CDS             33832..34038
                     /locus_tag="OO249_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635080.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            34038..35093
                     /locus_tag="OO249_000679"
     CDS             34038..35093
                     /locus_tag="OO249_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5635081.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQSDGKKWSEHDKDIYDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAEMACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            35105..35440
                     /locus_tag="OO249_000680"
     CDS             35105..35440
                     /locus_tag="OO249_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5635082.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLVDNGEFK
                     PWDGQESGKAVYLTSYLVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            35453..35866
                     /locus_tag="OO249_000681"
     CDS             35453..35866
                     /locus_tag="OO249_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5635083.1"
                     /translation="MATNESIMALPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQTVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            36028..36462
                     /locus_tag="OO249_000682"
     CDS             36028..36462
                     /locus_tag="OO249_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635084.1"
                     /translation="MIDKACFVSQQEIAEHFKVNRTTIRAWTKQGMPYLNADRGKSGG
                     YHIGHTLLWSSGKSRLEAIRYHVETSALEKIMFARLLSSERDEYSSEETEHRFDEGLQ
                     IYGYSPEDVSKARNKMAGFLAGWRHAVSVRRASMEQSADTEQ"
     gene            36743..37024
                     /locus_tag="OO249_000683"
     CDS             36743..37024
                     /locus_tag="OO249_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635085.1"
                     /translation="MTEAEILGLIRRAGGISQQTDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVALYRETEKITYHWKKPERLRR"
     gene            complement(37702..>40059)
                     /gene="yejO"
                     /locus_tag="OO249_000684"
     CDS             complement(37702..>40059)
                     /gene="yejO"
                     /locus_tag="OO249_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5635086.1"
                     /translation="NGGYQYIEMNGTAEYSVLNDGYQIVQMGGAANQTTLNNGVLQVY
                     GAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGGLAIAVDQKAGGAIKASTRVM
                     EAFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLEVMDGGTATG
                     VDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAIDTIL
                     DEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANVWAGT
                     MVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSLENSV
                     WAIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNTLSGN
                     GNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDAAFTL
                     GNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAELDTV
                     RERLGSVKSVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREESSTI
                     RGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHGKM
                     SNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGMRADV
                     GNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNHVKVNDDGKFNNDVAG
                     TPGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT        10077 a        10187 c        10023 g         9772 t
ORIGIN      
        1 gagcctataa ttacgacata atccttttcg cgcatagcac atcctctggt aaaaaattac
       61 caaccctgtc aatccacaag aatatagata ctgcaaagac ggtttaaaca tgtgttcata
      121 tttaaacatg tgctcatagc atgcatgttt tacgtaaagt aaattaccgc cagagaggca
      181 aaatgggcag atttgagcag gctcacaaaa tgcgattaaa tgcctgaatt acaaaagcga
      241 ggaatttgtg acgggatgca cagaacggga acaataaaaa aggcagaaaa taatctgccc
      301 ttgaggaaat aaccttagtg tttgatgaac gtttcaagtt tctactgaaa gataaaacca
      361 tttaaattca taaaattaca ttcacaaacc ggtcattctc tttgcgctca tcaaatgtta
      421 cagggcagga aatttctgcc ctgtaacaca ccttttatta cgctgctttc gctactgcgt
      481 ccacttccgg acgtttcagg aaggcatagg ccaaacccgc caccagcgta ccggcaataa
      541 ttgctaccag gtaacccagt accggcgtaa tagcgccagg gatcagcaga acaaacagac
      601 caccgtgcgg tgccatcagt ttcgcaccaa tcgccattga gattgcgcca gtcagcgccc
      661 cacccacgat acagcacggc agcacacgca tcggatcacg agcagcaaac ggaattgcac
      721 cttccgaaat gaagcacagt cccaatacca gagcggcttt gccaccttcc tgctgcgctt
      781 tgtcgaattt gcgacgcgcc accattgttg ccagacccat tgccagcggt ggcaccatac
      841 ctgccgccat aatcgccgcc atcgggccat aggtttgagt actcagcaga cccacaccga
      901 atgcgtacgc tgctttgttt accggaccgc ccatgtcagt acacatcatg ccaccgagga
      961 tcgcccccag cagaaccgca ttcgcagtcc ccatggtctg cagccagtga gtcagccctt
     1021 cgagaatgcc agcaactggt ttaccgatca ggtagatcat cgccagaccg accaccagac
     1081 tggaaattag cggaatgatc aggatcggtt tcagcgcctc catactctgt ggcagtttca
     1141 gttgcgtact gattaacttc gcaatgtaac cagccaggaa gccagcaatt ataccgccaa
     1201 taaagccaga accggtgctg accgccagca taccgccaat cagacccggc gtgaggcccg
     1261 gacgatcggc aatggagaag gcaatataac ctgccagtac cggcaccatc agcgcaaagg
     1321 ctgaaccacc gccaatctgc atcagcgccg ctgccaacgt acccggctct ttaaacgctt
     1381 cgataccaaa agcaaaagaa agcgcgatac acagaccacc tgcgacgacc atcggcagca
     1441 tataagagac gcctgtcagc aagtgacggt aagcgcctgc actctctttc ttaccttcag
     1501 tggtcgccgt ttgagctttg cccgccggtt catacggcgt tgcttcagca accgctttat
     1561 ccagttcctg cgcggttttc ttcagcgcca gaccggtaga ggtacgatac atcggtttac
     1621 cagcaaattt cgccagatcc acttcgatat ctgccgccac aatcaccaga tccgctgctg
     1681 cgacttcttc aggggtgatt gcattgcccg cgccaacaga accacgggtt tcaactttca
     1741 cccaccagcc acgttttttc gcttcggttt caatggcttc agccgccata aaggtgtgtg
     1801 ctacgccagt cgggcaagca gtcaccgcaa ctacgcgttt cggaccgctg gcagcaaccg
     1861 gtgctgtcgc agcgaccggc gcagtgtaag gtttcgcatg gcctttggct tcactcagga
     1921 acagctcagg gtgcgcaact gcccgggaaa tatcacccag ccagacattt ttaccgttca
     1981 gcgcgctgtc attcgggatg gaatcaccga gaacaatcgc catttctgcg tcgttcggat
     2041 tgtcgatgat ttccagtttt gcttttcgcg ccaccgcgcc cagcagggtc ttcgccatat
     2101 aggcgcgtgc ctgaccgaga ttagcgtcaa taatcagcag cgttttcatt atgcctctcc
     2161 tgctgtcagt taaaaggttg taagtcgacg cgcgccatca ttgcggccaa ctgcggacga
     2221 tcggtaatac ccacattgct ttgacttacc gccagggctg caacagctgt cgccagacgc
     2281 agtgtgtgtt cactggattc acgcatcagc aagccataaa tcaggccacc aaccatagaa
     2341 tcccctgcgc caacggtgct tacgacatcg actgacggtg gtttggcgat ccattcgccg
     2401 gaggcattaa cccaaagcgc gccttcggca cccagtgaaa taacaacatg cgcaatacct
     2461 tgttcacgta gcgcatgtgc agcttcaatc acatctttca tttcaggcag tttacggcct
     2521 gcccaaattt ccaactcgcg gcggttaggt ttcaccagcc acggtgccgc tttcaaacct
     2581 gctactaacg cttcacggct actatcaaag ataatgcaag gacactgact acgcaggcga
     2641 gtcatccagt cggtgaacgc ttccgggctg acgcctgacg gtaagcttcc gctgacacag
     2701 accatatcga actgaccgag ccagctcaga gaatcagtca caaagcgttc ccagtcggcg
     2761 ggggtgactt caaaacccga gaagttgaag tcggtcactt cgccgtcttt ttccgtcagc
     2821 ttaacgttaa ttcgggtgcg cccctgtaca acctggaaac ggttggcaat gcccagctcg
     2881 ctgaacagtt gctgaaaacc atcctgattg tctttaccca ggaagccgcc aacggtgaca
     2941 tcaattccca ggtcttttaa tactttggcc acgttgatgc ctttacccgc cgcatgcaga
     3001 ccggtggttt tcaccaggtt cacttcgccg cgttcaattt ccgggcagaa accaacaagg
     3061 tcataagccg gattaagggt gatcgtagca acacgtctgc tcattatgcg ccctccccaa
     3121 gaccagcagc gatagcgtcg ccaatggctt tcagcgcctg ttcagcatct gcaccctggg
     3181 cggtaaagcg taggcgatga cctttcttaa cgccaagtgc cacaactttc atcagactac
     3241 gtccgtttgc cggtttgccg gtgccatcaa ggtttgtcac ggtaatatca ctgttaaatt
     3301 gtttaatggt attgaccagc atggtacctg gacgagcatg caggccgtgt tcattgcgca
     3361 ccacaaactc cgcgcttaac acgtcgtcgg tcggcgcatc atcgctggtc agcagcgcca
     3421 gcaacgttgc cgcatctgct ttcagcaagc ggtcagcttt attgtcgagc aataaatcag
     3481 cgagacgctt aagaaccgcg atgggctgat cgtcattcat cgccacactc accagcatgg
     3541 ctgccgtttc gccgtccaca gcaaaagcat ttgccgcacg gcttaccgca atcgcgctac
     3601 gcagattgcc ttcggcgcta tcgctcagcc agataccctg tccgagattc agcggttgtt
     3661 cattgatggc tttggtgacg aaagtggcgt caactgcccc cgcctctttc agacgcgcag
     3721 cgttcagcgc ctgaagagtc agcagatcgc tggcgacgat atccagcgtc agcatttcgt
     3781 tgtcgagctt cagctgctca ctctgctttt cgcccatcag taatgcgcga agttcttctg
     3841 ctgttgttgc tgacttcagt tgttcagcaa cggaatcatc gctcagtacg tgggtcagct
     3901 ggcgtagcag gcccagatgt tcatccgagc tggcagcaat accgattgcc acgtacgcta
     3961 cctgaccgtc accccaggtg acgccttccg ggaactgaaa tacctgaacg ccggttttca
     4021 gcacctgatc gcgggtgtcg gtagtgccgt gtggaatagc aataccattg ccgaggaacg
     4081 ttgaggtttg ctgttcgcgc gccagcatgc cattgacgta gccttctgct acattaccgg
     4141 cctgcaccag cgccgcagcg acctggcgaa tcgcctcttc tttgtctccg gccttttcgc
     4201 ccggatggat gtcctgtacg gataactgga acatagttct cctctcttgc tgaattgaaa
     4261 cgattcagcc tctatgagaa aaaaagcgcc aacctggctt agggttaaag acaagatcgc
     4321 gctgaaacgt ttcaagaaag cataatactt ctgtttcagc acgcaaggaa agccgtaaag
     4381 ttatgagcaa aagtttgatg tgctgcacat ttttttgtca tttttcctaa ttgctgatgg
     4441 gaaaatccgt tatcagcaat ttcatttcag cagcgtcagc agccaatcag caacagtgaa
     4501 atggcatttt gattattcgt ggcgcttttg atttcgctgt ctgtttattt tctgacaagc
     4561 agcgtaaact ccgcgtcttc ctcttccagt gatagacctg catgcataac tcccccgcag
     4621 tctccagcgc gaaatcgttt gacctgacct cgacggcgtt tttaatcgtt gcctttctca
     4681 ccggtattgc gggtgctctg caaaccccga cactcagtat tttccttacc gatgaagtac
     4741 atgcccgtcc ggcgatggtg ggattcttct ttaccggcag cgctgtcatt gggattctgg
     4801 taagtcagtt tctcgctggg cgctctgata agcgcggcga tcgcaaatcg ctgattgtct
     4861 tttgctgcct gttaggcgtg ctggcctgca ccctttttgc ctggaatcgc aactactttg
     4921 ttttgctatt cgttggcgtc tttcttagca gctttggctc gaccgctaac ccgcaaatgt
     4981 ttgcccttgc ccgtgaacat gccgacaaaa ccggacgtga ggcggtgatg ttcagctctt
     5041 ttttacgcgc tcaggtttca ctggcatggg tcattggccc accgctggct tatgcgttag
     5101 cgatgggttt cagctttacg gtaatgtatc tgagcgcagc ggtagcgttt attgtttgcg
     5161 gcgtgatggt gtggctgttt ttaccgtcga tgcaaaaaga gcttccgctg gcgaccggca
     5221 cggttgaagc gccgcgccgt aaccgtcgcg atacgctact gctgtttgtc atttgtacat
     5281 tgatgtgggg ctcgaacagc ctgtacatca tcaacatgcc gctatttatt atcaacgaac
     5341 tgcatcttcc cgagaaactg gccggtgtga tgatggggac cgccgccggg ctggaaatcc
     5401 cgacgatgtt gattgccgga tatttcgcca aacgtctggg taagcgtttc ttaatgcgcg
     5461 ttgctgccgt gggtggcgtc tgtttttacg caggaatgct gatggcgcat tcacctgcca
     5521 ttctgttggg cttgcagctg ctaaatgcta tttttattgg cattctgggc ggcatcggga
     5581 tgctctattt tcaggatttg atgcccggtc aggcaggttc agccaccacg ctctatacca
     5641 acacgtcgcg cgtgggctgg atcatcgcag gatcagtggc gggcatcgtc gccgagatct
     5701 ggaattatca cgctgtgttc tggtttgcga tggtgatgat tatcgccact ctgttttgct
     5761 tactgcggac taaagatgtt taagacgcgg tcagcatttc cagatcgata agccaggtca
     5821 tggcctgctg acgcgagtta ccgcacattt cagcgctggc ttgtaagcct gcacagactt
     5881 tcggtcgcag tggcgaggtg aaaattttac agcgttgctg ttcatcaagc tgaatgcagg
     5941 gcgtattggc gggcttgcca tctggcatac cgggaatagg gctgaaaatt gaaggggcag
     6001 tgcaacacgc cccgcaaccc ggacggcatt ccatacgttg ttctcttaag gttaaccacc
     6061 ggattgatga gcgcgcacag taccaccttt tttgcaccag caaaagtgcg aataccactt
     6121 gcccgaaagg cccgtcgcga gtactttgtc gcgatatttt tgacattttc gactacagga
     6181 atttttcgat gccaagagcg aacgaaatta aaaaaggtat ggtactgaat tacaacggca
     6241 aactgctgct ggttaaggat attgatattc agtcgcccac tgcccgcggt gccgctacgc
     6301 tgtacaaaat gcgtttttct gatgtccgta ccgggctgaa agtagaagag cgtttcaaag
     6361 gtgatgatat cgttgacacc gtgacgctga cccgccgtta cgttgacttc tcctatgtcg
     6421 atggcaacga atatgtcttt atggataaag aagactatac cccgtatacc ttcaccaaag
     6481 atcagattga agaagagttg ctgtttatgc cagagggcgg catgccggac atgcaggtgc
     6541 tgacctggga cggtcaactg ctggcgcttg agctgccgca gaccgttgat ctggaaatcg
     6601 ttgaaaccgc accaggcatc aaaggggcat ctgccagcgc ccgtaacaaa ccggcgacat
     6661 tgagcactgg tctggtgatt caggtaccag aatacttaag cccgggcgaa aaaattcgta
     6721 tccatatcga agaacgccgt tatatggggc gtgctgacta acttcagccg catgcagaaa
     6781 agggatagct caggctgccc cttttttaat ttattatacc aatcttctat tttgcgcttc
     6841 ctgaacacct tacgccctca attttcactc gttgattgat cgccctcaca cttcatcgca
     6901 ttaacaatcc agaccaattt caattgctgt catataactt tacactgtca ttgttaattt
     6961 atcgttacta agacgtgact cctatgaaga caattgcctc cgttacgctc ccgcatcatg
     7021 tacacgctcc acgctacgat cgccagcagt tgcaatcacg tatcgttcat tttggctttg
     7081 gcgcctttca ccgcgctcat caggcgttac tgaccgatcg tgtgctgaat gcccagggcg
     7141 gcgactgggg gatctgtgaa atcagcttgt tcagcggtga tcaactgatg agccagctcc
     7201 gcgcacagaa ccatttatat accgtgctgg agaaaggtgc ggacggcaat caggtgataa
     7261 ttgtcggtgc cgttcacgaa tgccttaatg caaaactgga ttccttagcg gcaattattg
     7321 agaaattttg cgagccacag gtggcaattg tttccctgac gattaccgaa aaaggctatt
     7381 gtattgaccc ggccaccggt gcactcgaca ccagtaatcc gcggattatt cacgatctac
     7441 aaacccctga agaacctcac tccgcaccgg gtattctcgt cgaagcactg aaacgccgcc
     7501 gtgagcgcgg ccttacaccg tttaccgtgc tctcctgcga caatattccc gacaatggcc
     7561 atgtggtgaa aaacgcggtg ctgggaatgg cagaaaaacg ttcgccagaa ctcgccgggt
     7621 ggataaaaga acacgtcagt tttccgggaa ccatggtcga ccgcattgtt ccggctgcaa
     7681 ccgacgaatc actggcagaa atcagccagc atctgggggt aaatgatccc tgcgcgatta
     7741 gctgcgaacc gtttatccag tgggtggtgg aagataactt cgtcgctggg cgtcctgcct
     7801 gggaagtcgc aggtgtacaa atggtgaatg atgtcctgcc atgggaagag atgaaactgc
     7861 gtatgcttaa tggcagccac tcttttctcg cttatctggg ttacctttca ggattcgccc
     7921 atatcagtga ttgcatgcag gatcgcgcat ttcgccatgc cgccagaaca ttaatgctga
     7981 atgagcaagc gccgacacta caaattaaag atgtcgattt aacacaatat gcggacaagt
     8041 taattgctcg ttttgctaat ccggcgctga aacataagac ctggcaaatc gcaatgaatg
     8101 gtagccagaa attaccgcaa cgtatgctgg caggtattcg catacatctg gggcgcgaaa
     8161 cggactggtc gttgctggca ttaggcgttg ctggctggat gcgttacgtc agcggcgttg
     8221 atgatgccgg aaatgccatt gatgttcgcg atccgcttag cgataaaatt cgcgaacttg
     8281 ttgcgggcag cagcagtgaa caacgcgtaa ccgccctgct ttccctgcgt gaagttttcg
     8341 gtgatgatct gccagataac ccgcattttg tgcaggccat cgaacaagcc tggcaacaaa
     8401 tcgtacaatt cggcgcacat caggcgctat taaacaccct caaaatttaa cgatttctgc
     8461 ggttaaagcg gatgaagctc accttcgtcc gctctcccct tctcttttct gcctttttta
     8521 gccaggatta acgctcagtt aacttaccag agtattgcgg agccgttgtg accaggacca
     8581 acctcatcac cggttttctc ggcagcggga aaaccacgtc gattcttcat ctgttagccc
     8641 ataaagatcc caacgaaaaa tggacggtac tggttaatga atttggggaa gtcggaattg
     8701 atggtgcttt gctcgccgat agcggcgcat tgctgaaaga gatccccggc ggctgcatgt
     8761 gctgcgttaa tggtttaccc atgcaggtag ggttgaatac cttactgcgt cagggaaaac
     8821 cagaccgctt gttgatagag ccgaccgggc tgggccatcc gaaacagatc ctcgatctgt
     8881 taaccgcacc agtctatgaa ccgtggatag atctgcgcgc caccttgtgc attctcgatc
     8941 cacgcttgct gctggacgaa aaaagcgcca gcaatgaaaa cttccgtgac cagctggctg
     9001 ccgcagacat cattgtcgcc aataaatccg accgtgcgac gcccgaaagt gagcaagcgc
     9061 tacagcgttg gtggcagcaa aatggtggcg atcgacaatt aattcacagt gagcatggga
     9121 aagttgacgg tcatcttctg gatttgccgc gtcgcaattt agccgagttg cccgccagcg
     9181 ccgcgcattc tcatcagcat gtcgtgaaaa aagggttagc agcgttaagc ctgccagagc
     9241 atcaacgctg gcgtcgcagt ctgaacagcg ggcaaggata tcaggcctgc ggctggatat
     9301 tcgacgctga tacggtattc gacaccattg gcattctgga atgggcgcga cttgcaccgg
     9361 tggaacgcgt caaaggcgtg ctgcgtattc ccgaagggct ggtacgaatc aaccgtcagg
     9421 gcgatgacct gcacattgaa acgcaaaacg ttgcgccacc ggacagccgt attgagctga
     9481 tttccagcag cgaagctgac tggaatgcct tgcagagcgc gctgttgaag cttcgtttag
     9541 cgactaccgc gtaaggttgc ctgcgttttt cagtaagata attagagaaa atatgattaa
     9601 aaatttgccg caaatagtgt tgttgaatat tgtcggcctc gcgctgtttc tttcctggta
     9661 tattcccgtt aatcatggat tatggttgcc gattgatgcg gatatttttt atttctttaa
     9721 tcagaaactg gtcgaaagta aggccttttt atggctggtt gcattgacca acaatcgcgc
     9781 tttcgacggt tgttctctgc tggcgatggg aatgttgatg ctgagtttct ggctgaaaga
     9841 aaacgcccca ggcagacgac gtatcgtgat tattggtctg gtaatgctat taactgcagt
     9901 ggtattaaac cagctgggtc aggcattaat tcctgtaaaa cgggccagcc caacattgac
     9961 gtttactaat attaaccgcg tcagcgaact gctctctgtc cccacgaaag atgcctcacg
    10021 agatagcttt cccggcgatc acggcatgat gctgcttatt ttttcggcat tcatgtggcg
    10081 ttatttcggt aaagttgcag gtcttatcgc ccttattatt tttgtggttt ttgcgtttcc
    10141 cagagtaatg attggcgcac actggtttac tgacatcatt gtcggttcga tgaccgtgat
    10201 attgatcggt ttgccctggg tgttgctgac gccattaagt gatcgattaa tcaccttttt
    10261 tgacaaatca ctaccgggaa aaaacaaaca tttccaaaac aaataactca cagtaattaa
    10321 catcatcagg tttattttta tagtgaggat aatcctgatg atgcgcaccg tgctttcatc
    10381 tatcgaacgc aaaaatcatt ctctaagtaa atgaatggat tgcatgcgtt tcactcaatc
    10441 gtactttaat tgaccaatcc cgcttattaa ctttctgtat cactttttct tataaaaaat
    10501 catgtaaaac cgctcgccaa gaccgcacca atcgggtaat ctcgaactcg ttttgcctcg
    10561 gcggtagatt atcctcacag catataattt tgtgcgttag tccacagatt tggccttaag
    10621 gaattgtttc aacatgccca ggtaattagt ctcgtgtcgc ttggcatttt tttataacga
    10681 tatttgtcgt taaggacttc aagggaaaac aaacaacatg gtcaaatctc aaccgatttt
    10741 gagatatatc ttgcgcggga ttcccgcgat tgcagtagcg gttctgcttt ctgcatgtag
    10801 tgcaaataac accgcaaaga atatgcatcc tgagacacgt gcagtgggta gtgaaacatc
    10861 atcactgcaa gcttctcagg atgaatttga aaacctggtt cgtaatgtcg acgtaaaatc
    10921 gcgaattatg gatcagtatg ctgactggaa aggcgtacgt tatcgtctgg gcggcagcac
    10981 taaaaaaggt atcgattgtt ctggtttcgt acagcgtaca ttccgtgagc aatttggctt
    11041 agaacttccg cgttcgactt acgaacagca ggaaatgggt aaatctgttt cccgcagtaa
    11101 tttgcgtacg ggtgatttag ttctgttccg tgccggttca acgggacgcc atgtcggtat
    11161 ttatatcggc aacaatcagt ttgtccatgc ttccaccagc agtggtgtta ttatttccag
    11221 catgaatgaa ccgtactgga agaagcgtta caacgaagca cgccgagttc tcagccgcag
    11281 ctaataaacc gtttggatgc aatcccttgg ctatcctgac gagttaactg aaagcactgc
    11341 ttaggtagtg cttttttgtt ttcattcatc agagaaaatg atgtttccgc gtcttgatcc
    11401 aggctatagt ccggtcattg ttatctttta aatgttgtcg taatttcagg aaattaacgg
    11461 aatcatgttc atacgcgctc ccaattctgg acgtaagctc ctgcttacct gcattgtagc
    11521 aggcgtgatg attgcgatac tggttagctg ccttcagttt ttagtggcct ggcataagca
    11581 cgaagtcaaa tacgacacac tgattatcga cgtacaaaag tatctcgata cctattttgc
    11641 cgacctgaaa tccactactg accggcttca gccgctgacc ttagatacct gccagcaggc
    11701 taaccccgaa ctgaccgctc gcgcggcgtt tagcatgaat gtccgtacgt ttgtgctggt
    11761 gaaagataaa aaaacattct gttcatctgc gactggtgag atggacattc cactcaatga
    11821 attgattccg gcgctcgaca ttaataaaaa cgtcgatatg gcgatcttac ccggcacgcc
    11881 gatggtgccg aacaaacccg caatcgtcat ctggtatcgc aaccctttgc tgaaaaatag
    11941 cggtgtgttt gccgctctga atctcaacct gacgccttct ctcttttata gttcacggca
    12001 ggaagattac gatggcgtcg ccctcattat tggcaatact gcgctatcta ccttttcttc
    12061 acgtttgatg aacgttaatg aattaaccga catgcctgtc cgtgaaacta aaattgcggg
    12121 cattcctctg accgttcggc tttatgcgga tgactggaca tggaacgatg tgtggtacgc
    12181 atttttactg ggcggcatga gtggaactgt cgttggcctg ctctgctatt acctgatgag
    12241 cgtacgtatg cgccccggca gagaaatcat gaccgccatc aagcgcgaac aattttacgt
    12301 ggcgtatcaa ccggtggtgg atacacaagc tttgcgagta acgggcctgg aagtactgct
    12361 acgctggcgg catcctgtcg cgggagaaat tcccccggat gccttcatta actttgccga
    12421 atcgcaaaag atgattgtgc cgctgactca gcacctgttt gagttaattg cccgcgatgc
    12481 cgcagaatta gaaaaagtgc tgccagtagg cgtcaaattt ggtattaaca ttgcgccgga
    12541 ccatctgcac agcgaaagct ttaaagcaga tatccagaaa ctgctcactt ccctgcccgc
    12601 acaccatttc cagattgtgc tggaaattac cgagcgcgat atgctgaaag agcaagaagc
    12661 cacacaactc ttcgcctggc tgcactcggt cggcgtagaa attgctattg atgacttcgg
    12721 caccgggcac agcgcgctta tctatcttga gcgttttacg ctcgattatc tgaaaattga
    12781 ccgtggattt atcaacgcca tcggtacgga aacgatcact tcacccgtac ttgacgcggt
    12841 gctgacgctg gcgaaacgcc tcaatatgct gacggttgct gaagggggcg aaacgccgga
    12901 acaggcgcga tggctaagcg aacccggcgt taatttcatg caaggctact ggattagtcg
    12961 cccgttaccg ctggacgatt ttgttcgctg gctgaagaaa ccgtatacgc cgcagtggta
    13021 aggtgtgctt acgtccctta ttattcatag tgaaagcatg ccggattgcg gctaatgatg
    13081 agtaaaagga aatccgttgc agatgattgt gcgcatactg ctgctgttta tcgccctgtt
    13141 caccttcggt gcgcaggcgc aggctatcaa ggaaagctat gcctttgccg tgctgggcga
    13201 accccggtac gcatttaatt tcaaccattt tgattatgtg aaccccgccg cgccaaaagg
    13261 tgggcagata acgttgtcag ccctcggtac cttcgataat ttcaaccgct atgcactgcg
    13321 cggcaacccg ggcgcacgca ccgagcagtt gtacgacacg ctatttacga cttccgatga
    13381 cgaaccaggc agttattacc cgctgattgc tgaaagtgca cgctatgctg acgattattc
    13441 ctgggtggag gtcgctatta atccgcgcgc ccgttttcat gatggttcgc ccattactgc
    13501 ccgcgatgta gagtttactt ttcaaaaatt tatgaccgaa ggcgtgccgc aatttcgtct
    13561 ggtctacaaa ggcaccaccg tcaaagccat tgcaccgtta accgtgcgca ttgagttagc
    13621 taaacccggc aaagaagata tgctgagtct gttttcgctg ccggtatttc cagaaaagta
    13681 ctggaaggat cacaaactta gcgacccgct cgccacgcct ccgcttgcca gtggtccgta
    13741 ccgcgttacg tcctggaaaa tggggcaaaa tattgtctat tcccgtgtga aagattactg
    13801 ggcagcaaac ttaccggtaa accgtggacg ctggaatttc gacaccattc gctacgatta
    13861 ttacctcgat gataatgtcg cctttgaagc gtttaaagca ggtgcctttg atttgcgtat
    13921 ggaaaacgac gctaaaaact gggccacgcg ttataccggt aaaaatttcg ataaaaaata
    13981 catcatcaaa gatgagcaaa agaacgaatc agcccaggat acgcgctggc tggcgtttaa
    14041 tatccaacgt ccggtattca gcgatcgccg ggtccgggaa gcaatcactc tcgcctttga
    14101 ctttgaatgg atgaacaagg cgttgtttta caacgcctgg agtcgcacga acagttattt
    14161 tcagaatacc gaatacgcgg ccagaaatta ccccgacgcc gcggagctgg tgcttctggc
    14221 accaatgaaa aaagatctac cgccagaagt cttcacacaa atctaccagc cgccggtatc
    14281 taaaggcgat ggctacgatc gtgacaacct gttaaaagcc gacacacttc tcaacgaagc
    14341 gggctgggtg ctgaagggtc agcaacgcgt taatgccaca acgggtcagc aactcagctt
    14401 tgaattattg cttcccgcaa gcagcaatag tcagtgggta ttgccgttcc agcacagcct
    14461 gcaacggctg ggtatcaaca tggacattcg caaggtggat aactcgcaaa tcaccaaccg
    14521 catgcgcagt cgcgactatg acatgatgcc gcgcctatgg cgggcgatgc cgtggcccag
    14581 ttccgattta cagatttcct ggtcatcgga atatatcaat tccacttata atgcccccgg
    14641 cgtgcaaagc ccggttatcg actcactgat caaccaaatt attgccgcgc agggaaataa
    14701 agaaaaatta ttgccgttgg ggcgagcact ggatcgcgta ttaacgtgga attattacat
    14761 gctgccaatg tggtacatgg cggaagaccg tctcgcctgg tgggataaat tctcccaccc
    14821 cgctgtacgc cctgtttaca gcctgggtat cgatacctgg tggtatgacg ttaataaagc
    14881 ggccaaactg ccgtcagcca ggcaacaggg agagtagatg ggcgcttacc tgattcgccg
    14941 tctgttgctg gtgatcccaa cattatgggc gattatcacc atcaactttt tcatcgtgca
    15001 aattgcgcct ggcggtccgg tcgaccaggc catcgccgcc attgagtttg gtaatgccgg
    15061 agtattaccc ggcgcaggcg gtgaaggtgt tcgtgccagc catgcgcaaa cgggtgtcgg
    15121 caatatcagc gacagtaatt accgtggcgg acgcggatta gatccagaag tgatcgctga
    15181 gatcactcat cgctacggtt ttgataagcc gatccacgaa cgttacttca aaatgctctg
    15241 ggactacatc cgctttgatt ttggtgatag cctgtttcgc agcgcctcgg tgctgacgct
    15301 gattaaagac agtctgccgg tttccatcac cctcggattg tggagcacgc tgattatcta
    15361 tctggtgtcg attccgttag gcattcgcaa agctgttcat aatgggagcc gctttgacgt
    15421 ctggagtagc gcatttatca ttatcggcta cgccattccg gcctttttgt ttgccatcct
    15481 gctgattgtc ttcttcgcgg gcggcagcta tttcgacctg ttccctctac gcggcctggt
    15541 ttccgctaac tttgattcgc tgccgtggta tcagaaaatc accgattatc tgtggcatat
    15601 cacgctgccg gtgctggcga cggtgattgg tggctttgcg gcgctgacca tactgacaaa
    15661 aaactcattc cttgatgaag tgcgcaagca atacgtggtg accgcgcgtg cgaaaggggt
    15721 aagtgaaaaa aatattctct ggaaacatgt gttccgcaac gccatgctgc tggtgattgc
    15781 cggttttccg gcgacgttta tcagcatgtt ttttaccggc tcgctgctga ttgaggtgat
    15841 gttttcactc aatggtctgg gcttactggg ctacgaagcg accgtctcgc gcgattatcc
    15901 tgtaatgttt ggtaccttgt atattttcac cctgattggc ctgctgctga atattgtcag
    15961 tgatatcagc tatacgctgg ttgatccgcg tattgatttt gagggacgtt aatgtcgcga
    16021 ctcagccccg tcaatcaggc ccgttgggcg cgttttcgtc ataaccgtcg cggctactgg
    16081 tcgttatgga ttttcctcgt cttgtttggt ttgagtttgt gttctgagct tatcgccaac
    16141 gataaaccat tgctggtacg ttatgacggc agttggtatt tcccgttgtt gaaaaactac
    16201 agcgaaagcg attttggcgg cccgctggca agtcaggctg attatcagga cccgtggctg
    16261 aaacaacggc tggaaaataa cggctgggta ctgtgggcac cgattcgctt tggtgctacc
    16321 agtatcaact ttgctaccga taagcccttc ccttctccac cctcccggca aaactggctg
    16381 ggaacggatg ccaacggcgg cgatgtgctg gcacgcattc tctatggcac gcggatctcg
    16441 gttctgtttg gcctgatgct gactctctgt tccagcgtga tgggcgtgct ggcgggggcg
    16501 ctacaaggct attacggcgg taaagtcgat ctctggggac aacgctttat tgaagtatgg
    16561 tcggggatgc cgacgctgtt tttgattatt ttactttcca gcgtcgtaca gcctaacttc
    16621 tggtggctgc tggcaattac tgtcttgttt ggctggatga gtctggtcgg cgtggtgcgg
    16681 gcggagtttt tacgtactcg taatttcgac tacattcgtg cggcgcaggc gcttggcgtc
    16741 agcgatcgca gtatcatcct gcgtcatatg ttgcctaatg ccatggtcgc gaccctcacc
    16801 tttttaccgt ttattttatg tagttcgatc accaccctga cctcactcga tttcctcggc
    16861 ttcggtctgc cgctcggttc accgtcactc ggtgaactgc tgttacaagg gaaaaataac
    16921 cttcaggctc cgtggcttgg gatcaccgcc ttcttgtcgg tggcgatatt attgtctttg
    16981 ctgatcttta ttggtgaagc cgtccgcgac gcatttgatc ctaataaggc ggtgtagcat
    17041 gacgcaaact ctgttagcga ttgaagattt gtcggtgggt tttcgccatc agcaaaccgt
    17101 acgtacagta gtcaatgatg tttcactaca gattgaggct ggcgaaacgc tggcgctggt
    17161 gggtgagtca ggttcaggca aaagcgttac cgcgctgtca attttacgcc tgctcccttc
    17221 cccgccggtt gaatatctct ccggcgatat tcgttttcgt ggcgaatcgc tgcttcatgc
    17281 cagcgatcaa acgttgcgcg gtgtacgcgg taataagatc gccatgattt ttcaagagcc
    17341 gatggtgtcg ttaaatccat tgcataccct ggaaaaacag ctttatgaag tgctttcact
    17401 ccaccgcggg atgcgtcggg aagcggctcg aggcgaaatt cttaactgcc ttgatcgcgt
    17461 tggtatccgc caggcggcaa aacggctgac agattatccg catcagctct ccggcggcga
    17521 acgccagcgg gtgatgattg cgatggcgct gttaacgcga ccggaattat taattgccga
    17581 tgaaccgacc accgcactgg acgtctctgt ccaggcgcag attttacagc tgttgcgcga
    17641 actgcaaggc gagctgaata tgggcatgct gtttattact cataacctca gcattgtcag
    17701 aaaactggcc caccgcgtgg cggtaatgca aaacggtcgc tgtgtcgagc aaaattacgc
    17761 cgctacgcta tttgcctcac ccactcatcc ttacacacaa aagctactca acagtgaacc
    17821 gtcaggcgat ccagtgccgt tgccagaacc tgcctcaacg ttgctggatg ttgaacagct
    17881 tcaggttgcc ttccccattc gcaaagggat tttgaagcgc attgtggatc ataatgtggt
    17941 ggtgaaaaac atcagtttta cgctacgagc gggtgaaaca ctgggtttag tgggcgagtc
    18001 cggttccggg aaaagtacga cgggactggc gctgctgcga ctgattaatt ctcagggcag
    18061 catcatcttt gacggtcagc cactgcaaaa tttaaatcgc cgccagctgt tacctattcg
    18121 tcatcgcatt caggtggtat ttcaggatcc aaactcctcg ctcaacccac gactcaacgt
    18181 tttgcagatt attgaggaag gcttacgggt tcaccagccg acgctttctg ccgcacaacg
    18241 cgaacaacaa gtgatagccg tgatgcatga agtgggatta gatcctgaaa cacgccaccg
    18301 ttatccggcg gagttctctg gtggtcagcg acaacgtatt gcaattgcca gggcattaat
    18361 tcttaagccc tcgctgatca tacttgatga accgacatca tcactcgaca aaacggtaca
    18421 ggcgcaaata ttgacgctat tgaaatcatt gcaacaaaag ctgcaactgg cctatttgtt
    18481 tatcagccac gatttgcacg ttgtccgcgc gttatgtcat caggttatcg tactgcgaca
    18541 aggggaagta gtggaacaag gaccgtgcgc gcgcgtgttt gccgcaccgc agcaggagta
    18601 tacgcgtcag ctactggcgt tgagctgacg cttaaaaagg attgtagttt gaaaagggtt
    18661 cggcaattgc cacaccaaaa tttttcaatc ggcatgttgc agcaaactcg tcctggcgat
    18721 ttacaaacag gcacggctca ccttcacact ccagcaccga acacggaact tcgatgtcgc
    18781 ttaacgcctg gcttagtttg tacatcaccg accatgcatt atcaccatcc gggctaagca
    18841 atttaagcgc caccaggctg ttgtcaccgc acggtccatt ttgcggaatc ggttcaacct
    18901 tcgaacctgc gaaaccagca gaccagcgaa agttctgcgg tagacgatgg acgattgaga
    18961 gttgtaatga agtcacttat tttccccgga agcacattta cttcacaatt tgtttacatc
    19021 aattttaaca catcatcaac aatccgtctt tatacagatc gtaaagattg atgcctcgcc
    19081 tgcgtggcct ctatggctct gatttaagta atatctgcgg gcgtggtcac gtttgcgggg
    19141 ctatatgtac ccgtttctgc gatctaactc aaccttttta actacaatga tgtgactttt
    19201 tacataaatt gattttacat aaaataaaca tatatcgggg gaatgataga tttgtggttg
    19261 acatgcatca acaaaggaag ccttttagct tcctcgttgt gcaatagatc accgtttttt
    19321 cggtcgactg gcgtacagac agaagagaat ggagctgctt gcgcagaatg caattgacca
    19381 aatcatcggc catgcagagt taaaggtcgc aagagaaagc aatgcgccaa caattgcccc
    19441 tatgccaaaa cggaaggttc ctgccagcga agatgccgtt cccgccatat ggggaaactc
    19501 atcaagaatg accgccatcg cattggatga caccatcgac acacagccca caaacgccgc
    19561 aacgccaacc accagaatcc cagccccagc agcgcactga tgaccatcca cgctgccata
    19621 ataaattgta tccacaaccc cgagcggaac atatttaacg cgccaatgcg gcgaacgaag
    19681 cggctgttaa agatggtcat cacgaacagg aaaacaatgt ttagtgcaaa gtaataacca
    19741 aagttttccg gcgcgacgtg gttaatttca atgtagacaa acggtccggc gctcaggaac
    19801 gagaacattc cggcaaagct gaaaccgctg gccagcatat aactcaggac acgcttatga
    19861 cggaacagcg ccgcaaagtt accaatagta gtacgaatgt gaaaaggctg gcgacgctcc
    19921 ggtggtaagg tttctttgat cagaaagaag atcattgccg aagccagaat cgccgccacg
    19981 gcgaggatcc agaagatgta atgccagctc agccacacca gcacccagcc gccaactatc
    20041 ggtgccatca gcggtgcaat ggtcgtcacc agcatgacaa acgacatcat ccgcgagaac
    20101 tcttctttcg ggtagatatc acgcatcaaa gcgttgataa ccatgctggc cgcagccgca
    20161 gccagcccgt ggaaaaaacg catcacaatc agctgatcga tggtttgtgc caacgcacac
    20221 gccaccgcgg cggcggcaaa caccagcgta ccgccgagca ccaccggctt acgcccgaag
    20281 ctgtctgcca tcggcccgta gattaactgc cccaacgcaa agcccagaat ataagtactg
    20341 agggtcatct gcgtactgcc cgccggtacg ccaaactgcg ctgaaattac cggtagcgcg
    20401 ggcagataca tatcaatcga cagcggcatc aacatggcca gcaggccaag gataaaaaca
    20461 atagcaaacg acgaatgctg tcgggtggtc acaacgggct cctgaaagtc attgaaaagt
    20521 tagacgacgc tggcaatttc ttcttcagtt aacggacgat attcaccggg ggctaaatca
    20581 gcatccagcg taataccgcc aatacgttca cgatgcagct caaccacgtg gttacccacg
    20641 gcggcgaaca tgcgtttcac ctgatgataa cgcccttcgc tgatggtcag acgaacctgc
    20701 gttggggtaa tcacttccag caccgcaggc ttagtgagat ctttttcgtt atgcagctgc
    20761 acgcctttag caaattgctc tgccgtatcg tcagctacag gtgattccag tgtcaccaga
    20821 taggtcttct cgcaatgatg gcgcggagaa gtaatgcggt gcgaccactg accatcatca
    20881 gtcatcagca ccagaccggt ggtatcgata tccagacgtc ctgcggcgtg cagtttccag
    20941 gctaccggtt catccagaaa atagagcacc gttgggtgat cagggtcgtc cgtggagcaa
    21001 acatagccct gaggcttatt gagcatgaag taacgtggac catgttgctg cgccagcggg
    21061 ttgccatcgt aagcgacatc atgttcagga agcagtttga acgctgcatt acgaacaatt
    21121 tcgccatcga cggtgacacg attgccgcgg atttcacgcc cggcaatagc acggctaacg
    21181 ccgagttgct gtgcgataaa tttatcaagt cgcatgtgtg tgattttgcc tgtaaaaacg
    21241 gaggtcgggc attgacccga aaatctgaac tgttgtctgc ccagtatagc ggtctaataa
    21301 catccctcaa gggaaaaaga tccatggcat actattagca gaataatcta cctacgcgag
    21361 accatgattt ttacactccg cccatatcag caagaagccg tggatgccac gctcaaccat
    21421 tttcgtcgtc ataaaacccc tgccgttatc gtgctgccca ccggcgcagg taaaagcctg
    21481 gtgattgccg aactggcacg gctggcgcgt ggtcgtgttc tggtgctggc acacgtcaaa
    21541 gaactggtgg cgcaaaacca tgcaaagtat caggcgctgg ggctggaagc cgatattttt
    21601 gccgctgggt taaagcgcaa agagagccac ggtaaagtgg tatttggcag cgtgcagtcg
    21661 gtcgctcgta atcttgatgc ctttcagggt gaattttcgc tgttgattgt cgatgaatgc
    21721 caccgtattg gtgacgatga agagagccag tatcagcaaa tcctcactca cctgactaaa
    21781 gtgaatcccc acttacgcct gctggggctg actgccacgc cttttcgatt gggcaaaggc
    21841 tggatctacc agttccatta tcacggcatg gtacgcggcg atgagaaagc ccttttccgt
    21901 gactgcattt atgagctgcc gctgcgttat atgattaaac acggctatct gacgccgcca
    21961 gaacgactgg atatgccagt agtgcaatac gatttcagcc gcttgcaggc acagagtaac
    22021 gggctgttca gcgaagctga tctcaaccgt gagctgaaaa aacagcaacg cattacgcca
    22081 catatcatca gccagatcat ggagttcgcc gaaaagcgca aaggggtaat gattttcgcc
    22141 gccaccgttg aacacgcaaa agagattgtg ggattactgc ctgccgaaga tgcagcactg
    22201 attactggcg acacccccgg cgctgagcgc gatgtgttaa ttgaagattt taaagcccag
    22261 cgttttcgct atctggtcaa cgtcgcggta ctgaccaccg gatttgacgc cccgcacgtc
    22321 gatcttatcg ccattctgcg tcctaccgaa tcagtgagtc tttaccaaca aattgtcggg
    22381 cgcggtctgc gtctcgctcc tggcaagact gattgcttaa ttcttgatta tgcgggtaat
    22441 cctcacgacc tctacgcgcc ggaagttggt acaccgaaag gcaaaagtga caacgtcccg
    22501 gtacaggttt tctgccctgc ctgcggtttt gccaacacct tttgggggaa aacgaccgcc
    22561 gacgggacat tgattgaaca ctttggtcgt cgctgtcagg gatggtttga agatgacgac
    22621 ggccatcgcg aacagtgtga cttccgtttc cgttttaaaa attgcccgca atgtaacgcg
    22681 gaaaacgata ttgccgcccg ccgctgccgc gaatgtgaca cggtactggt tgatccggat
    22741 gatatgttaa aagcggcgct acgactgaaa gacgcgctgg tattacgctg tagcggcatg
    22801 tctttacagc atgggcacga cgagaaaggc gaatggttga aaatcaccta ttacgatgaa
    22861 gacggcgcgg atgtgagtga gcgtttccgt ctgcaaacgc ccgcccagcg aactgccttc
    22921 gagcagcttt ttatccgccc gcatacgcgc acaccgggca tccctctgcg ctggatcacc
    22981 gccgccgata tcctcgccca gcaagcctta ctgcgacatc cggattttgt cgtcgcccgc
    23041 atgaaaggtc agtactggca ggtgcgtgaa aaagtgttcg attacgaagg tcgttttcgt
    23101 cgggcgcacg aattacgcgg ttaatggtag ttttgattga tgtataaggc gattgagtat
    23161 agaatctcgc ccgcttttgc atacgcaaag cagatcactt acctgttgct gggtcgcctg
    23221 tagcaggatt aatttaagag agaaagaaat gtttactatc aacgcagaag tacgtaaaga
    23281 gcagggtaag ggtgcgagcc gccgcctgcg tgccgctaac aagttcccgg caatcatcta
    23341 cggtggcaaa gaagcaccgc tggctatcga gctggatcac gacaaagtca tgaacatgca
    23401 agctaaagct gaattctaca gcgaagttct gaccatcgtt gttgacggta aagaaatcaa
    23461 agttaaagct caggacgtac agcgtcaccc gtacaaaccg aagctgcagc acatcgactt
    23521 cgttcgcgct taattgctga ataagttgta aaaaaccccg ctccggcggg gttttttgta
    23581 tctgcagatt atgcctgatt acggtattgc tattttttgc aggccagata aggcgttcac
    23641 gccgcatctg gcattcagaa tacaaaaccg attaattgcc gccagacgtc cggcgctgca
    23701 attggtcgcg caaattcggc ggcgtgcctt taatggtcaa ggtatcggtt gctggatccc
    23761 agaagatgcg ttcacctaac agcatcgcat caaagttaat agtcaagcca ccgccgctac
    23821 cggcaaactt cgtcaactga cgcagtgtgc tacggtctgc cgggaagctc tcttccagtt
    23881 cgtagccttt ttcagccgca aacttcgtga agctcacttc gcttacgcct gccagctctt
    23941 tcgacagcga ttccagttcg atctcttcgc ctgcctgcag ctgctcatta cagtagctat
    24001 aaacctgctg gcgcacgttc tgtcgctctg ctttatccag ttgtgcttcg gcagtgaaat
    24061 catcaaccgc ttgcaacagc ccacggtttt gcgctttcgc gttcaatcct tcgctggccc
    24121 cgaggaaatc cataaagaaa tccgccactt tgcgccctac tcgccctttc aggaaagtga
    24181 gatagcgggt ggactctgga ttggtttccc attcggttaa atcgatacgc gcaacaatat
    24241 ccgcatgatt gatatcaaga taatgggttg ggttgatgtc gagattttcg ttaacgcgca
    24301 tactgctcag gttgctcaga actgccacca gcagatactc caccgccaga taacggtagt
    24361 ggcaaaacag cacgaatcca ccgtcagcaa aaggatattt cgctaattcg tcacgcaggc
    24421 gcccggttgc cgcgcggcta aatgccagaa agtcctcttc gccctgacgc tgtaagcgca
    24481 gcgtctgcgc cagttcactc tcttcgctaa acaagccgta agctttattt ttggcgctat
    24541 agacccgatg cagttctgcc accatctcaa cgacggtttc tgtcggttcc agcaatgaat
    24601 cgcgcaacac cagctcaagg ttctgctcat cacgcttgat aagctggtgc agagcaatct
    24661 ggttgatatc cagactcatg ataaactctc ctttaagacc gggcggtatt caaccaccgc
    24721 cggttaagcg acgcaataaa agatggcggt cattttacag aaggcagcgt aacgagaatg
    24781 atttaacgca taaaaaagca gaaaaaaaac cgttgctacg gtaatatgtt gccctttcat
    24841 caactaactg atttcgattt atgccacaaa tttcccgcta cagtgatgaa caggttgaac
    24901 aattgctcgc agagctgctc aacgtactgg aaaaacataa ggctccgacc gacctttccc
    24961 tgatggtgtt aggcaacatg gtcactaacc ttatcaacac cagcattgcc ccggcccaac
    25021 gccaggcaat tgccaactct tttgcccgcg ccttacagtc ctctatcaac gaagacaaag
    25081 cgcactaagg gaaacagata acaggttatg gtaactcatc gtcagcgcta ccgtgaaaaa
    25141 gtctcccaga tggtcagttg ggggcactgg tttgcactgt tcaatattct gctttcgctc
    25201 gtcattggca gccgttacct gtttatcgcc gactggccga caacgcttgc tggtcgcatt
    25261 tattcctacg taagcattat cggccatttc agcttcctgg tgttcgccac ctacttgctg
    25321 atcctcttcc cgctgacctt tatcgtcggc tcccagaggc tgatgaggtt tttgtccgtc
    25381 attctggcaa cggcgggaat gacgctatta ctgatcgata gcgaagtctt tactcgtttc
    25441 catctccatc ttaatcccat cgtctggcag ttggttatca acccagacga aaatgagatg
    25501 gcgcgcgact ggcagctgat gttcatcagc gtgccggtta ttttattgct tgaactggtg
    25561 tttgcgacgt ggagctggca aaagctgcgc agcctgacgc gtcgtcgacg cttcgcgcgc
    25621 ccgctggccg cattcttatt tatcgccttt atcgcctcgc atgtggtgta tatctgggcc
    25681 gatgccaact tctatcgccc gatcaccatg cagcgcgcta acctgccgct ttcgtacccg
    25741 atgacggcgc gacgtttcct tgagaagcat ggcctgcttg atgcgcagga gtatcaacgc
    25801 cgtcttattg agcaaggtaa tccagacgcc gtttccgtac agtatccgtt aagcgaactg
    25861 cgctatcgcg atatgggcac cgggcagaat gtgctgttga ttactgtcga tggcctgaac
    25921 tactcacgct tcgagaagca gatgcctgcg ctggcaggtt ttgctgagca aaatatttcg
    25981 ttcacgcgcc atatgagctc cggcaacact acagacaacg gcatctttgg cctgttctat
    26041 ggcatctcgc cgagctatat ggacggcatt ctgtcgaccc gtacccctgc ggcgttaatt
    26101 actgcgctta atcagcaagg ctatcagctg ggattattct cgtcagatgg ctttaccagt
    26161 ccgctgtatc gccaggcatt gttgtcagat ttctcgatgc cgagcgtacg cccccaatcc
    26221 gacgagcaga ccgccacgca gtggatcaac tggctgggcc gctacgcaca agaagataac
    26281 cgctggttct cgtgggtctc tttcaatggc actaacattg acgacagcaa tcagcaggca
    26341 tttgcacgga aatatagccg ggcggcaggc aatgtcgatg accagatcaa ccgcgtgctc
    26401 aatgcactgc ttgattctgg caaactggac aatacggttg tgattatcac tgccggtcgg
    26461 ggtattccgc tgagcgaaga cgaagaaacc tttgactggt cccacggtca tctgcaggtg
    26521 ccattagtga ttcactggcc aggcacgccg gcgcagcgta ttaatgcgct gactgatcat
    26581 accgatctga tgacgacgct gatgcaacgc ctgctacatg tcagcacacc tgccagcgaa
    26641 tattcgcaag gtcaggattt gttcaaccct caacgccgtc attactgggt cactgcagcg
    26701 gataacgata cgctggcaat taccaccccg aaaaagacgc tggtgctgaa caataacggt
    26761 aaataccgca cttacaactt acgtggtgaa agagtgaaag atgaaaaacc acagttaagt
    26821 ttgttattgc aagtgctgac agacgagaag cgttttatcg ctaactgatt aattataaat
    26881 cagttagcga aatatcttac ttgcaatcgg tgtggaaaac ggtagtatta gcagccacga
    26941 gtcggcacgt agcgcagcct ggtagcgcac cgtcatgggg tgtcgggggt cggaggttca
    27001 aatcctctcg tgccgaccaa atcgcccacc ggataccacc ggaattaaca gtttttagac
    27061 acgaaaagcc cgctaatcct taattagcgg gtttttttat ggtttttagt caccggacat
    27121 caccggattt ataagctatc agccggattt tttagttata cgcttagtta tacgggcttg
    27181 cgtataacta aagccgtata actaaaaccc tgttttttcg tgtggaatca tatacatatg
    27241 gcgagaaaaa cacccccatt aaccacggtg cagatcaaag cagccagacc agcggaaaag
    27301 gaatacaccc tacaggacgg cggagggctt tttctcctgg tcaaaccgtc cggatcaaaa
    27361 ctctggcgat tttcctacta ccggccatcg gacaaaaaaa gaatattgct gagttttgga
    27421 tcgcttgatg atgtttccct ggctgatgcc agaaaacgcc gtagcgagta cagagcgtta
    27481 atcagtgccg gaactgaccc gcagggccat gagaagaaaa aacgagaggc agaggcccga
    27541 agacaaggga acacgttcga aaatgtggcg gcggcatggt accaggtgaa aatcagccag
    27601 aatctggccc ccaacacgat taaagacatc tggcgttcgc tggataaata tgtattcccg
    27661 ttcatcggca acacgccaat agataccctc accgcccgaa ggttcgttga agtgcttaca
    27721 cccatcaagg agcgcggcaa cctggaaaca ctcaagcggg ttttacagcg cgttaatgag
    27781 gtaatggatt acgccgccaa cagtgggctg attgatgcca atccggctat gaatgtgcgt
    27841 aaggcgtttc cctcacctgt aaaaaaacat atgccaacaa tccgccccga acagctgccg
    27901 gagcttatgc aggctttatc agtatcggca acagaacggc agaccagatt actgattgaa
    27961 tggcagttac tgaccgtaac ccgtcccgcc gaagcgtcat caacgcggtg ggatgaaatc
    28021 aacctggacg cgaagcaatg gacgatacct gccggacgca tgaagatgcg cagggatcac
    28081 gttatcccgc tttccggtca ggctatggcg gtgctggagg ccatgaaacc aatcagccac
    28141 caccgcaatt acgttttccc aagtctgaaa gacccacagc agccgatgaa cagccagaca
    28201 gctaacgcag cattgcggcg tatgggattc gctggcgtgc tggtgtctca tggattacgc
    28261 gccatattca gcacagcagc gaacgaggaa ggattcgagc cggacgtaat agaggcggca
    28321 cttgcccacg tcgacaccaa cgaagttaga cgggcataca accggagcaa ctacatagaa
    28381 aaacgcatcg tgctgatgcg ctggtggggc gaatttgtcg aggctgcggc gacgggcgta
    28441 accctcgcca gtggtaaaag gggtatccga gccgtgtagc tgtacagaaa accagtaaaa
    28501 actacgaaaa ccatgtaaaa ccgtcgtata attgcatcaa atttaacgac aaggccgtga
    28561 aacatgaaac cgttaagatg caaaaaaata tcagatgcaa ttgcgacggg ctgcaactgg
    28621 ccctgatggt tcagcatgaa ttttggtcaa cctacgatcc ggaggacaga acgacggccc
    28681 catcaaaaaa acaggtagta gattttctgg tatcccgtgg cgcttccaga aatctggcgg
    28741 taagtattga taaggtcgta cgtccggcat ctatgaagat cggaggcagg cccaaaaaat
    28801 ggcggtaaca atcctggaag cggcagaaat gccgcttttt ttataattcc atttcaaatc
    28861 atcaatataa aaacggtgta taccgtttaa aaacggtggg aactgttttt acccatatcc
    28921 gatgatttac cgtatttgtc accggaatac accggattca caaggtaaat cacgatggaa
    28981 gcaatcagaa aaattatctt tcgccaggaa gtaaaaaaaa ttatccacat caaggcagac
    29041 agcacgctgc aaagcatgat caacgccgga gaatttccgc agggttttcg cgttggttta
    29101 cgccgtcgcg gatggtatga ggatgatgtg ttggcctggc agaaagaacg cgaacaggaa
    29161 gcacgcggaa cggctgctta acgggtatca cagatatgac aaacacgaaa aaagcggccc
    29221 cgatatggag ccgcctttct gaacaattaa cccgctgcgc cttatgtgta tatgatccca
    29281 aacataagca cggggatgat agccgctatc aggctggtgg gcaatgcaat cagtctggtt
    29341 cagttcgttg ccatacctgc aatgagcgct tttccctata ctctttaagg aattgctcaa
    29401 gggcaaaagc acatggtgcg aatctttctg attcatgctc tatctttctg cgccgtcttt
    29461 tccgtgccgg tgataatgtt ttggtcaatt ctttatcggt cattgtgttg tcctgcatag
    29521 caatgcgccg taatacctca caccacggcg ctgatagttt ttatcctttg ggttctatgc
    29581 cgcgccgctt tagttcagtg cgccccaact ctttaagcca gttggctagg cttatgccgt
    29641 cgctctgtgc ttctttgtcg aattgctctt ttagctctgg agaaattcgc attctgaatt
    29701 gtggggattg cccgtcccct ttagggcttt tatcgcgttt gattattgac aagtggtcac
    29761 ctattgaatt agcctttcca ttgttaggtg gccaccttaa cacaagagca cttaaaaaag
    29821 caaagccccg caagtgtcat taccactcgc agggcttcta accaacaacg taaactagga
    29881 gccgttatgg ttgccgtaaa tcatatacca caccttgtac acacacaaac ggcctttgtg
    29941 tggcgttttc tggccctgag tgccggagaa tctcaaatca tccacgtaac cgcctggacg
    30001 gaacgcgaag cgcgtagccg ttgcccgtcc ggttgtgttg ctgtattcgc cgcccgtatt
    30061 cgtcaggggg aaacctatgc acaataaaac cacaccgaac gcagcagccg ccgcactcac
    30121 tacgctgatg cacgcgctta ttgatattga atgcactgca gagcttgcgc agggagaaga
    30181 acagaaagac cggacacagt tcgccctgga atgtatccga tacatcgcaa cgcggtcgct
    30241 gaatgacgct aaaaatattc ttgttgctga ttgtgaaaat ggggggggtt atgcgtgatg
    30301 atcgttttaa ttccctgaaa caggaatttc ccggcgttcc tgatgatgcg gctgatgcgc
    30361 tttcgtcaat gccagaactt attagagcgg cttttttctt actttccacg agagaatata
    30421 aatcaacggg gcttgatgta ctgaatatcg ccgccgatta tgcggaatat gtggcagagg
    30481 cgcgttacag aagaaaattt cctgaggatg taagccatgc gtgatattta ccacgaaaca
    30541 atagaccgcg catttcttgc actttctcac agtgaaaaca tgctggaaat attgcgcata
    30601 tggcttgaaa cacttggcga caatgaacgc gacaaacaaa aatcaagaat tgccacggca
    30661 ttaataacgc ttcttgagcc tgtaataatg gaactgcaag aaatagatct attgcacgac
    30721 agatataaag aacagcacac cggagaataa aaataatgaa acttaaatat tctggcttaa
    30781 ctgccagtgg caacactcac cctaaattta cgcgcggtga tatttaccgc gaccagtacg
    30841 gcggcacggt aatgattaag ggcgtggaag aacggcgtgt aacctaccgc cgtgaaggtt
    30901 acgaatatga ttgcgtgatg cctgtttatc agttccggcg tgatttttct ctggtacaga
    30961 ccgcgccgca taacgtgccc accagcaacg ccagggcacg ggcaaacatc cagaagctga
    31021 aaaccatgat taacggattc aggggcaaga aatgaaaagc gcaccgaact taaaaaaaca
    31081 gccttacgac aagatgaccg aagtcattat ttttgcgggt agtgatgcct gggcacatgc
    31141 gaaacagtgg caggaacagg acgggcgact ggctggcgat aatgtgcctc ccgttgtgct
    31201 ggctgatgat caactggatg aactggcaga cctgagaatc atcgacgagg ggcgctattg
    31261 tgtccggctg tacaaggcag gccacatcag gccatcaaat attaatgcca ttgcgcacaa
    31321 gctggcggcg gcgggtgtaa ctgatgcgaa ttattacccc gaagggatgc acagccatat
    31381 gcgggagaac tggcgcgaat acctggaacg ggtgcgcggg aaagagccgg tggaagaaaa
    31441 aaaccaccag cgaaaaacca cgctaccgat gagcgttgga tctaccggat acgacacgca
    31501 actggattac gtggttaagg ggattattcc ggcggtatcg ctatgcagca tatacggggc
    31561 tagcgggtcc tataaatcat tccttgccgg atcgtgggcg tgccatgttg ccactggtcg
    31621 ccagtgggga ggccgcaggg ttgcacatgg tgcggttctc tatgtggttg gtgaaggcgg
    31681 tatcggcgtt ccgcgtcgtg taaaagcctg ggaggttgtg cacgatgagc aggtgaaaaa
    31741 tctgtatctg gtaaaccgcc ccatctttcc ggctgccccg cttgatgttg atgaaatggt
    31801 tatcgctgcc cgtcaggtgg agcgggaaac gggtaaacct gtacgcatga ttattctgga
    31861 tacgctggcg cgttgctttg gtgggaatga tgaaaatgat tcccgtgata tgggggcgtt
    31921 tatccgtggt tgtgacgaac tgaaacgacg cacaggggcc acggtgctgg tggttcacca
    31981 ttccggcaag gatgagacga aaggcgcgcg cggttccagt gcatttcgtg cttcgctgga
    32041 tgctgaatac cggatacgca gggaggacgc aggaagcgaa gcgctggtta tctcatgcac
    32101 caaaatgaag gacgcggagg aactcaaaga agccgcatat gacttacgcg tggtggagct
    32161 ttttaccgac gctgacggtg aattaatcac gtcgctggtg gtggtggatg atccgcgccc
    32221 tcctgttgaa ctggagcgca tcgaggaggc agggaacaag acggaaaacc ataccgcgct
    32281 atgggggtgc atccgttcac gcacacagaa cggcgacaag tgcacgatcc cgctgttacg
    32341 tgatgacatg aaaaagctgg ggtatgaaat gaaaaacttc cggcgctggc tgtacaagct
    32401 ggaaaaagat ggggttatta gtatcgatgg ggatgatgta gcgccgctat aaaagtgagg
    32461 agcaaaagcg agggggatag aaagagggcc aaaattagcc cgctctccct cacttttcga
    32521 cctgtataca tcctcaaaag tgaggggtaa aaaatactta taaaacacac acatagaaaa
    32581 accgaaaatc ccaactgcga cgaagtgaga cgcttgaaaa agtgaggcga aaaagtgaga
    32641 ggttgcgaga aatgacccaa aaacgcagag acagaacaga gccaaaatat aaagcgttag
    32701 acatgactga gcacacctta aaggtggcaa tcagaacgat agaccgccac acgcgggaag
    32761 gatacgcgaa ggaacatccc gacctgataa gcgcattcat gaccacggcg gcggcaaact
    32821 ttgccacgct gacagaacgg gagattgccg aagcggaaca ggtaacaacc atcaacgtta
    32881 aaaccggaga ggtggaatta tgacggcaca gatagccgct tacgggcggc tggtggacga
    32941 cccgcaggta aaacagacca gcaagggcac accgatgacg ctggcgcgta tggcggtatc
    33001 tttgccatgc agccaggcac aggacggaca ggcgacgtta tggttatcgg tcatcgcatt
    33061 tggcaaacag gccggcttcc tggctaaaca tcaaaaaggc gatgttgcca gcgtatccgg
    33121 cacgatgcag gtaagccagt ggaccggaca gaacggagaa acgcggcagg gctggcaggt
    33181 tatcgcagac agcgtaatca gtgcccgcgc ggcacgtcca ggcgggaaca gacgcaaaac
    33241 cacaggcaca cagggtaatc agccaccagc gggaggcgat gacccttacg gtgatgatat
    33301 ttcgttctga gggggtgacg atggtacatg accgcatagc ggaggaactg gaggcgaaag
    33361 gcttttaccg gagggcggcg gcgcgatggg gtgaagtcat gcagctggtg gagacagaca
    33421 aggaacggca tcacatcacg atgcgacggc tggaatgttc aaggaaggca cagagggcac
    33481 cggagccgcc ggataatttc ggagacctga aaaaggcagt cgatcgcact tatgccgaaa
    33541 tgggtataga tggtgctggt gatgaaatat ggcgcaatta ccaggacagc taatcaaaca
    33601 gccggagaaa tccggctttt ttgcgcccaa aaaaagcccg ataaggtcag agggttctta
    33661 tcgggctttt gcatatgagg tttttggatg cactggcgtt cgtgatcggg ataatcattt
    33721 cataatttgc aacataactc aatattattg cataaaatgc aattctgatt ataatcagga
    33781 ctggataaac atccagttat gattttttta gtcgaagagg aatttcttac tatggctgaa
    33841 gagaaaaaag gcggtgtttc ggtgtacata agccccgaaa tcgtggaggt gctcaagcag
    33901 cgccacaaaa aaaactatga ggctggcgtg gcggctggac tggatccgct gatgacgccg
    33961 gagccgtcga taggttcact tgtacgctct tatttacttg cggcgcttgg gatgcataaa
    34021 aattatgggg gtgaataatg gcaggcaaag caacggcact taacactaac cagcttttta
    34081 tgtacctgaa tcgcggggat attgcggatt ttaaattcag ccctctgttt accacgctgt
    34141 ttttcccgaa cgtggcgaca ttcagcacgc aaaacatcat gctggatacc ctggacattg
    34201 aagaagtcac catgtcggcg ttttgttcgc ctatggtggg tagccaggtt cagcgcgata
    34261 aagggtacga aaccagcaca atcaaacctg gctacatgaa gccaaagcac gaaatcgatc
    34321 caacgaaaac catcatgcgc atggctggag aagatccggc acagcttaac gaccctacct
    34381 atcgccgtat gcgcctgatt actggcaaca tgcgccgcca gataaacgcc attaaggcac
    34441 gcgtggaatg gctggcggtg aatgcggtaa cgaccggaaa aaacatcatt gagggcgaag
    34501 gcatagaacg ctatgaaatc gactggaaga taccggaaaa aaacatcata gagcagtccg
    34561 acggcaaaaa atggtccgag catgataaag atatttacga tccaatctat gacatcgaac
    34621 tatacgcaga tcaggcaggt tgccccgcca acgtcatgat tatgggcgct gaggtatggc
    34681 gcacgttacg cagctttaaa aaattccgtg aactgtacga tctttcccgt ggttcagaat
    34741 ccgccgcaga aatggcctgt aaaaacctgg gcgaagtggt gagctttaaa ggctatctgg
    34801 gcgatattgc tcttattgtc tattccggca aatacactga cagcgacggc accgaaaaat
    34861 atttccttga gcctgatttg ctggtcctgg gcaacaccaa caataaaggg ctggtggcct
    34921 atggtgcgat tatggatcag gaagcggtaa gaacgggcgc aacgcaaaac atgtactacc
    34981 cgaaaaactg gattgaggac ggcgatccgg cgattgagta cgtgcagaca cacagcgcac
    35041 cgcagccggt tccggcagat attcgcaaat ttgttaccgt caaaattggt taacggggga
    35101 ttatatggac actccatata ttgagttatt tgcaggcagt cagcaggtat caacgacact
    35161 ggtacatttt gccgctgatg ctggcgttat tcaggaattt accccgctga tgctggtgga
    35221 caatggcgag tttaagccgt gggatggtca ggaatctggc aaggctgttt atctgacttc
    35281 gtacctcgtg gacacgtcaa agcagaaatc agcacagtgt tacaagacgg ggatattcaa
    35341 tattgccgcc gttaactggc ctgagagcgt cgacaccgat gcgaaaaaat gcgccgcctt
    35401 tgcgggttct ggcgtatccg ttcagccgct ggcccgataa gcagggggaa cgatggcaac
    35461 gaatgaaagc atcatggcgc taccgctggc gagtaaattt aaagccgaag cgcgggcaat
    35521 ggctgacaga ggtttatcaa cctacgaggc cgtatatcaa ctcaacaaac tggaagagca
    35581 ggacaagccg cgcgctgatg cgattatggc gcttcatgag cataaagact atcagccgct
    35641 gttgcgtgca atggcaaacg tgccttgtat cgatgttgat acggctaaaa acatcctgaa
    35701 catgaccata gagcaggaac gcccgaaggt tgcaccagag cttaccgcag cctttgaaaa
    35761 ctttatggac atgcacagcc cacaaaccgt atcagctggc atggcatacg atggcagaaa
    35821 cccaggcgat gacggcgaca tcgatcgcat actgaaaacc atctgagaca aggccggaga
    35881 aatccggctt ttttgcgggt ccttcctgga attatggccc gttacggggc ggcgacctcg
    35941 cgggttttcg ctatttatga cgtttttccg tgaaggtgac accaccacca cttgattaat
    36001 atttaaccat gcagttaagg taacattatg attgataaag cttgttttgt aagtcagcag
    36061 gaaatagctg aacatttcaa ggttaacaga accactattc gcgcatggac caaacagggg
    36121 atgccgtatc ttaatgcgga tcgcggaaag tctggcggtt atcacatcgg gcatacattg
    36181 ctttggtctt caggtaaaag ccgtcttgag gccatcagat atcacgtaga aaccagtgcg
    36241 ctggaaaaaa ttatgtttgc caggctgctt tcatctgagc gtgacgagta ctccagtgaa
    36301 gaaacagaac atcgatttga tgaaggtttg cagatttacg gctattcacc ggaagatgtg
    36361 agcaaggcac gaaataaaat ggctggcttt ctggctgggt ggcgtcatgc cgtaagcgtt
    36421 cgccgtgcca gcatggagca atcagccgat acagaacagt aaagccgata acttgttaat
    36481 cactcgcagc aatgcatgtg atttgtgcac actttcggtt aactggttcg atgtccggtt
    36541 ttagtgtctg ttttttgcgc atgtccggtt catggaaagc atgtttttat atttttcata
    36601 tggttaactt gtagagaaac cggacatgga tcccgaaaaa ttttcataaa tagtgaaaac
    36661 gcgcgaggtc gccgccccgt aacgggtcca taattccagg aaggacccga cgacaccaga
    36721 caatcagaac gataggtgca caatgacaga agccgaaata ctgggattaa tccgccgtgc
    36781 tggtggaatc agccagcaga ctgacgaaca ggccacgcag ccggacagcg tgacagccga
    36841 aaattatgcg cgtgttgttg ctgaggtgat gcgccgtgat ggtatccagc ttaatgatgt
    36901 ggatatgcgc aacatacgga tccgcgttct tgaaatgctg gcctacaatc gccgcgtggc
    36961 actgtatcgg gaaacggaga aaattacata ccactggaag aagccggagc ggttgcggcg
    37021 gtaactggtt gatattttcg aaattcgcca gcggcgaaca tcggggaaac cagcctaacc
    37081 cgttgatatt ttcgggaacc tcaaattgag gaggtcggaa acataacccg tcatattctg
    37141 gatgctgata attaccacgc agcccattaa ggcctaaccc gttgattctt tcggaaacag
    37201 ccattggcgt aatccggcgc ggtaacttgc tgaacttaaa gcaaagcgca aaattgcgct
    37261 ggctggtggg catgatgccg atatggtgat ccccataacg gaaatatcca ccagcacggc
    37321 aacggcagaa tagccacaac acagaaaaac cacgaatatg gggtttttgt tatggcatgg
    37381 gcatgatgac cgcccctgct ctgaatggtg gaaatcacca cgcagatcat ccaccagctt
    37441 cgtttttata aattaaacat ccgggaaaat ccgaccctga attgtgcatt ttttcagcaa
    37501 atcattttag ttatacgttt ggttatacgg tttaaaagtt gaatcatata cattatttta
    37561 aatcatgaag ttacacgctc aattaaacta ctctcgtgaa aaatcccaag aaaaaaccaa
    37621 cctttacggt tggttttttt atatctgcaa ttaatttgat aaacagaccg tgacacatca
    37681 cagcctgttt attttctgtt atcagaacgt ccagaccact cccgcctgag tattccacgg
    37741 cgattctacc cctgcgccat tgccatagct gactgacaaa tgaccgctta acgtcggggt
    37801 aaacgatgac cttatcccag cctgataaac gccaggggtt ccagccacat cattattaaa
    37861 tttgccatcg tcattaactt tcacatggtt agaatcggcg tattcctgac gtacggcggc
    37921 tttcagccag ggttccagcg tcgtaccatt ttgcaggtcc atgtgatagc ttaccgccgt
    37981 tcccgcttca gcgcgtaata tccgggtatt tcccacatca gcgcgcatgc cgtttgataa
    38041 cgtgtagtcc tgaccatctg tggtaaagcc ggtaaaggcc agatagggtc taacactcca
    38101 caatccgtca acccaacgga acccgctctc aacatgagca cccgcgccgt tactattgta
    38161 atcgccaaac gctgttgccc cattactcat cttgccatgg atggtgttgg caaaacggtc
    38221 aactttcacc accccatcaa cataggcacc gttctgatgc tcccaaccgg cataagcccc
    38281 cagggtatag ctatcgatat taccttttcc gccgcgatca aaaccaatat cagaatgaga
    38341 gtaaccaaag aacaagccgc gaattgtact gctttcttca cgggagaaac ggctatcgat
    38401 accgagcgtc aggcccgtca atgtttgctc aaaaccagct cccgcatcag tggtcacgtt
    38461 gttgcgggtg ttaattgccg aactccacat cgccgtatcg taactaacgc tttttacgct
    38521 accaagacgc tcacgcacgg tgtccagttc tgcatcaaat accagcggtt gtgcggccgc
    38581 catattcagc acatcagtgg ttgaaggggt aatttgcgcg cgattctctg ccaaactcca
    38641 gctatggttg ccattatcca gcaaggtata ttcatacgta ccgatatcaa caacgcctcc
    38701 ggcattgccc aacgtaaatg cagcatcacc gccgcccgtt gttaccagtg taaggctatc
    38761 tcctgctgcc gggctggcac cggtgtccgt cacgaatatt ttgaaatcac ctgttgcctg
    38821 accggtgacg ttgagctgat cgctctgatg attagccata tcggtacgca tataaaaatt
    38881 gccgtttccc gacagggtat tggtggtcaa cgtaatgaaa ttttccgcac ttgccgtcac
    38941 tgatgaacga gtcactggct catccatcat aatcacattt gcgtcagaca tcgcaaggtt
    39001 ggctaagttg aggagggtgt tttggttcgt cgtagtgata tcggcaatga tggcccatac
    39061 gctattttcg agcgaaacgt ccgctttgct ggtatccacg gcaccatgcg ttctaaaaga
    39121 agcgccctca gaaaccacta ccttacccac caacatagcg ggtgcataat ttatttgcgg
    39181 cttcatgacc tcaagaatgc catcattccc tctgactgac acgttaacca ttgtgccagc
    39241 ccagacatta gcgcggccat tgttgataac catattttca gagatggctt tagaggaata
    39301 cgtgatactt ccattctggt ctgatcgacc gagatcatat accgcatttg cctgcacttt
    39361 cgtaccagta tcctttccca gcgattgcat agtggcatgc tcatcaagga tagtatcaat
    39421 ggcctgcgtg tcctccataa caatgagccc ggaaccatca tccagttcat aattttttga
    39481 cacaccatct tttatactaa attggccttt actgtttgta ccactcactt ccagcgcatt
    39541 cgttgagaca attaattttc cgcctgcttt tttatcaacg ccagttgcag tcccgccatc
    39601 cataacctcc agtaagccgc cactatctac agtagtatta taagcgaagt cattttcttc
    39661 aactcgcaaa cttccgccgt tttccaacag catattgtta gcaataccat tcttgatttc
    39721 gaactgaccg agacggtttg ttccgaatgc ctccatgacc cgcgtgcttg ctttaatagc
    39781 accgcctgct ttctgatcta ccgcaatcgc taatcccccc tctttaacct ccagtaatcc
    39841 tcccttttcg atagcggcaa ggacggtaat cccatctttt tcaacgatta agcgcccgcc
    39901 tttaatcgtg ggatcattcg ctgcgccata aacctgtagc acaccattat tgagggtagt
    39961 ctggtttgcc gcgccaccca tttgaacaat ttgataaccg tcatttaata ctgagtattc
    40021 tgcggtacca ttcatttcaa tgtactgata ccctccgtt
//
LOCUS       DAJTOR010000014        39605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTOR010000014 DAJTOR010000000
VERSION     DAJTOR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.014"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            5..436
                     /locus_tag="OO249_000685"
     CDS             5..436
                     /locus_tag="OO249_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5635087.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFA
                     VQSGPMLLENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            519..1421
                     /locus_tag="OO249_000686"
     CDS             519..1421
                     /locus_tag="OO249_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR5635088.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKSGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1466..3628)
                     /gene="uvrD"
                     /locus_tag="OO249_000687"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1466..3628)
                     /gene="uvrD"
                     /locus_tag="OO249_000687"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5635089.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCLPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3712..4428)
                     /gene="yigB"
                     /locus_tag="OO249_000688"
     CDS             complement(3712..4428)
                     /gene="yigB"
                     /locus_tag="OO249_000688"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5635090.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4428..5324)
                     /gene="xerC"
                     /locus_tag="OO249_000689"
     CDS             complement(4428..5324)
                     /gene="xerC"
                     /locus_tag="OO249_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5635091.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5321..6028)
                     /locus_tag="OO249_000690"
     CDS             complement(5321..6028)
                     /locus_tag="OO249_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5635092.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6025..6849)
                     /gene="dapF"
                     /locus_tag="OO249_000691"
     CDS             complement(6025..6849)
                     /gene="dapF"
                     /locus_tag="OO249_000691"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5635093.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6894..7088)
                     /locus_tag="OO249_000692"
     CDS             complement(6894..7088)
                     /locus_tag="OO249_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5635094.1"
                     /translation="MKNVFKVLTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGMVHPR"
     gene            7551..7871
                     /gene="cyaY"
                     /locus_tag="OO249_000693"
     CDS             7551..7871
                     /gene="cyaY"
                     /locus_tag="OO249_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5635095.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7911..10457)
                     /gene="cyaA"
                     /locus_tag="OO249_000694"
     CDS             complement(7911..10457)
                     /gene="cyaA"
                     /locus_tag="OO249_000694"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5635096.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLCGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10844..11785
                     /gene="hemC"
                     /locus_tag="OO249_000695"
     CDS             10844..11785
                     /gene="hemC"
                     /locus_tag="OO249_000695"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5635097.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11782..12522
                     /gene="hemD"
                     /locus_tag="OO249_000696"
     CDS             11782..12522
                     /gene="hemD"
                     /locus_tag="OO249_000696"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5635098.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            12544..13785
                     /gene="hemX"
                     /locus_tag="OO249_000697"
     CDS             12544..13785
                     /gene="hemX"
                     /locus_tag="OO249_000697"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5635099.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPQGDTPAAAPQGE"
     gene            13788..14984
                     /gene="hemY"
                     /locus_tag="OO249_000698"
     CDS             13788..14984
                     /gene="hemY"
                     /locus_tag="OO249_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5635100.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15663..15824
                     /locus_tag="OO249_000699"
     CDS             15663..15824
                     /locus_tag="OO249_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635101.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15908..17143)
                     /locus_tag="OO249_000700"
     CDS             complement(15908..17143)
                     /locus_tag="OO249_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5635102.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17290..17366)
                     /locus_tag="OO249_000701"
     tRNA            complement(17290..17366)
                     /locus_tag="OO249_000701"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17330..17332),aa:Pro,seq:tgg)
     gene            complement(17409..17495)
                     /locus_tag="OO249_000702"
     tRNA            complement(17409..17495)
                     /locus_tag="OO249_000702"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17459..17461),aa:Leu,seq:cag)
     gene            complement(17516..17591)
                     /locus_tag="OO249_000703"
     tRNA            complement(17516..17591)
                     /locus_tag="OO249_000703"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17556..17558),aa:His,seq:gtg)
     gene            complement(17650..17726)
                     /locus_tag="OO249_000704"
     tRNA            complement(17650..17726)
                     /locus_tag="OO249_000704"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17690..17692),aa:Arg,seq:ccg)
     gene            complement(17829..19214)
                     /locus_tag="OO249_000705"
     CDS             complement(17829..19214)
                     /locus_tag="OO249_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5635103.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAVGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19405..20145)
                     /gene="rffM"
                     /locus_tag="OO249_000706"
     CDS             complement(19405..20145)
                     /gene="rffM"
                     /locus_tag="OO249_000706"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5635104.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20148..21500)
                     /gene="wzyE"
                     /locus_tag="OO249_000707"
     CDS             complement(20148..21500)
                     /gene="wzyE"
                     /locus_tag="OO249_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5635105.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21497..22576)
                     /gene="rffT"
                     /locus_tag="OO249_000708"
                     /gene_synonym="wecF"
     CDS             complement(21497..22576)
                     /gene="rffT"
                     /locus_tag="OO249_000708"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5635106.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRVLAIAAGEVA"
     gene            complement(22573..23823)
                     /gene="wzxE"
                     /locus_tag="OO249_000709"
     CDS             complement(22573..23823)
                     /gene="wzxE"
                     /locus_tag="OO249_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5635107.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23825..24955)
                     /gene="rffA"
                     /locus_tag="OO249_000710"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23825..24955)
                     /gene="rffA"
                     /locus_tag="OO249_000710"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5635108.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24960..25634)
                     /gene="rffC"
                     /locus_tag="OO249_000711"
                     /gene_synonym="wecD"
     CDS             complement(24960..25634)
                     /gene="rffC"
                     /locus_tag="OO249_000711"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5635109.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25612..26493)
                     /gene="rfbA"
                     /locus_tag="OO249_000712"
     CDS             complement(25612..26493)
                     /gene="rfbA"
                     /locus_tag="OO249_000712"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5635110.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLVQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDQERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26512..27579)
                     /gene="rffG"
                     /locus_tag="OO249_000713"
     CDS             complement(26512..27579)
                     /gene="rffG"
                     /locus_tag="OO249_000713"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5635111.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27579..28841)
                     /gene="wecC"
                     /locus_tag="OO249_000714"
     CDS             complement(27579..28841)
                     /gene="wecC"
                     /locus_tag="OO249_000714"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5635112.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28838..29968)
                     /gene="wecB"
                     /locus_tag="OO249_000715"
     CDS             complement(28838..29968)
                     /gene="wecB"
                     /locus_tag="OO249_000715"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5635113.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELVANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30024..31070)
                     /gene="wzzE"
                     /locus_tag="OO249_000716"
     CDS             complement(30024..31070)
                     /gene="wzzE"
                     /locus_tag="OO249_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5635114.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31082..32185)
                     /gene="wecA"
                     /locus_tag="OO249_000717"
     CDS             complement(31082..32185)
                     /gene="wecA"
                     /locus_tag="OO249_000717"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5635115.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32425..33684)
                     /gene="rho"
                     /locus_tag="OO249_000718"
     CDS             complement(32425..33684)
                     /gene="rho"
                     /locus_tag="OO249_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5635116.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33769..33870)
                     /gene="rhoL"
                     /locus_tag="OO249_000719"
     CDS             complement(33769..33870)
                     /gene="rhoL"
                     /locus_tag="OO249_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5635117.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34011..34340)
                     /gene="trxA"
                     /locus_tag="OO249_000720"
     CDS             complement(34011..34340)
                     /gene="trxA"
                     /locus_tag="OO249_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5635118.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34471..35736
                     /gene="rhlB"
                     /locus_tag="OO249_000721"
     CDS             34471..35736
                     /gene="rhlB"
                     /locus_tag="OO249_000721"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5635119.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEILEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35870..37354
                     /gene="gppA"
                     /locus_tag="OO249_000722"
     CDS             35870..37354
                     /gene="gppA"
                     /locus_tag="OO249_000722"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5635120.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37401..39422)
                     /gene="rep"
                     /locus_tag="OO249_000723"
     CDS             complement(37401..39422)
                     /gene="rep"
                     /locus_tag="OO249_000723"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5635121.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKYYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9821 a        10778 c        10016 g         8990 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtt gcgggagata aagtcggcat cattcgtctg gatgccttta aaaccagtaa
      121 agagattcag tttgcggtgc agtcagggcc aatgttatta gaaaacggcg taattaatcc
      181 acgcattcac ccgaatgttg cctcgcgtaa aattcgtaac ggtgtaggga ttaataaaca
      241 agggaacgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagtt gctttatctt gatggcacaa tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttaagcat tgtacactcg aggaggccag acaaactggg actggctaca
      481 cagctggaaa atcatcagat aaatgaaggg aaacaaaaat gaaaaaaacg ctcgcaacgt
      541 tatttttatt aacgtgtctg ggatcatcat ccgcctatgc ggataacgct ttgattttac
      601 aaactgattt tagcctgaaa gatggtgccg tctcggcgat gaaaggggtc gcattcggcg
      661 ttgaccataa tcttaaaatc tttgatttaa cacacgaaat tccaccgtat aacatctggg
      721 aaggcgctta tcgcctgtat cagactgcca gttactggcc gcaaggttcg gtgtttgtta
      781 gcgtggtcga tccgggcgta ggcactgacc gtaaatcggt agtgctgaaa accaaaagcg
      841 gtcaatactt cgtttcacca gataacggta cgctcacttt ggtggcacaa actctgggta
      901 ttgatagcgt ccgggaaatc gacgaaaaag ctaaccgcct gaaaggttct gaaaagtcat
      961 ataccttcca cggtcgtgac gtgtatgcct acaccggtgc acgtctggct tccggggcga
     1021 ttacgttcga acaggtgggg ccagagcttc cagcaaaagt tgtcgaactt tcttatcaga
     1081 aagcaaaagc aacaaaaggt gaagtgaagg gcaatatccc gattctcgat attcagtacg
     1141 gcaacgtctg gagcaatatc agcgacgagt tgctgaatca ggcagaaatc aaactcaacg
     1201 acacgttgtg tgtgaccatt tccgaaggtt cccagcaaaa atacgcaggg aaaatgccgt
     1261 atgtcgcaag cttcggcgat gtgccggaag gccagccgat ggtgtatctg aacagcttat
     1321 tgaatgtttc cgtggcgctg aatatggata atttcgcgca gaaacatcag gttgcgtctg
     1381 gtgctgactg gaatattgat gtgaagaagt gcgctaagta aggtgctgat gcggaatgcg
     1441 gtgtggcgca ccgcatcccg caacgtcaca ccgtctccaa ccgggcataa gccgccacca
     1501 gccatttaat accctggccc tggaatgcca cctgcaaacg gctatgttca ccgctgcctt
     1561 ccatattgac gatggtgcct tcaccaaact tagcgtggcg tacacgctgg ccgagcttgt
     1621 agccgctgtc gttctcgacc atcggcgtac ccatccgctg atggctgacc gggcggctta
     1681 ccgtggcgcg caggcgcacc tcttccacac actcttccgg cagctcgcca ataaagcgcg
     1741 acgggcgatg gtaaacctct ttgccataca gacggcgagt ttccgcgtag gtcagcgtca
     1801 gtttctgcat cgcgcgggtt acgccaacgt aggccagacg gcgttcttct tccagacgcc
     1861 cgccttcatc cagcgacatc tggcttggga acatgccctc ttccataccg acgataaaca
     1921 cctgcgggaa ctccagacct ttcgccgagt gcatggtcat cagttgtacc gcatcctgcc
     1981 aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg ggataggaac gcctgcagcg
     2041 gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg cgttgccgtc accagttcct
     2101 ctaagttttc gatacgcgtc tgaccttttt cgcccttctc ctgctcgtac atggtacgca
     2161 ggccggagtc tttaattacc cggtcagtct gtacatgcag cggcatatcg gcagtttcct
     2221 gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc gctggcagca cgtccggcga
     2281 gggctttttc ctgcaacagt tcacgacatg cctgccagag tgttaactgg cgatcgcgcg
     2341 atgtctgacg taccacgtcc agcgtccggt caccaatacc ccgcgttggc gtattcacca
     2401 cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag gcgcagatac gagagcgcat
     2461 ctttgatttc ctggcgttcg aagaagcgca tcccgccgta aatacggtac ggcatactcg
     2521 cctgcaacag tgcttcttcc agcacacgcg actgggcgtt actgcggtag agaatggcgc
     2581 actcggcaag cgccccgccg ttgtcctgcc aggttttgat gcggttaacc acaaaacgcg
     2641 cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg ctcaccgtcc gcgccatcgg
     2701 tccacagttt tttacccaga cgcccgttat tgttttcaat cagggcgtta gcggcgctca
     2761 gaatattgct ggtagagcgg tagttttgct ccagacgaat agtttcggca ccggggaaat
     2821 cattaaggaa acgctgaata ttctccacct gcgccccgcg ccagccgtag attgactggt
     2881 cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc cagcaggcgg atccacgcgt
     2941 actgaatgtt gttggtatcc tggaattcgt ccaccaggat attggtaaaa cgttcgcggt
     3001 agtgttgcag gatatgcggc ttgttaagcc acaactcgtg agcgcgcagc agcagctcgg
     3061 cgaagtccac caggcccgcg cggtcacacg cttcctgata cgcctgatac accttctgcc
     3121 aggtctgctc caccggatta ccgtagcttt gaatatgatg tggacgcagg ccttcatctt
     3181 tctggctgtt gatgtaccac attgcctggc gcggcggcca ctgcttctcg tcgaggttca
     3241 tcgccttgat taggcgtttg agcaggcgta gctggtcctc gctgtcgagg atctgaaaat
     3301 cctgcggcag attggcgtcc atatggtgcg cacgcagcag acggtgcgcc agcccgtgga
     3361 aggtgccgac ccacatgccg ccctggctgg tgcccattag ctgcccgata cgatgacgca
     3421 tctccgccgc cgctttgttg gtaaacgtca ccgccataat cgagtatggc aagcagtttt
     3481 ccacgctcat cagccaggcg atacgatgca ccagtacgcg cgtcttacca ctgcccgcgc
     3541 ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc ttcgcgctgt ttgtcattaa
     3601 ggctgtcgag caggtaagaa acgtccattg gcaccgccgc gtaaaaatag gttgggcgga
     3661 gaagcgcaac gccctccgcc aaaaagctgg gtatatatac agatttgctg attatatcag
     3721 cgaggtcaga gatgccaacc gggaaatttc cagatgcggc agtaaacggc tgtcccaggt
     3781 ttgcatcaga tcgccatttt ccggtctgat ccaacaggcc tgcattccgc tgcgaattgc
     3841 cccacccacg tcagtggtga gatcgtcccc aacatgtaag atctcgccga tcggcacgtt
     3901 gagtttttcc gcagccagaa agtacatatc gctgaacggt tttgagcgcc cgtgcgggcc
     3961 agcgcgcagc acaaactcaa aataatcccc caaaccaaac agttccggct gggcgttacc
     4021 gttggtgatc gccaccagcg gccatttctt cgccagctgt tttaaggtgt cgtgagtttg
     4081 ctgcgggacg tcgattcggc tgcgccattt ggcaaagttg atcattgctg cgtgtgcgcc
     4141 tgcactggct tcttcggcac tcagcccggc gtcgagcatc gcttgttcaa tcgaacgaaa
     4201 acgccagcgc gtcacgtcgt gataaatctc gggttccgct tcccgtaccg cctggcgcag
     4261 gcgttgcaga tcttcattct ggaagctgcg cagcgccgga tgataatttt gcacaaaggt
     4321 aagcgcctct cgctcggtgc gcaaaatcac cggacggtta tcgtaaaggg tatcatccag
     4381 gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa aaacgcatta tttcccccgt
     4441 ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt gttgaaaatc aagatgagta
     4501 tagatttgcg tggtggagag gttggcatga cccagtagct cctgcacacc acgaagatcg
     4561 ccgctcgact ccagcatatg cgtagcgaac gagtgacgta atttatgtgg gtgaacgtga
     4621 ttattcagcc cttgttttat gccccattcg gcaaagcgtt tctgcacatt acgcgcggag
     4681 atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt cgctaccaaa caggtcgcgc
     4741 aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac caatcggcag gcggcgctct
     4801 ttgctgcctt tccccattac ccacacttca ccagactcca ggtcgaggtg tttgatatcc
     4861 agccccacca gctcggaaag acgcagacca gcgccgtaca tcacttccag cattgcacgg
     4921 tcgcgtacag cgaggggatc gttgatatca atatccagca gccgattcat atcgtcgacg
     4981 tcgatatttt tcggcagatg acgcggcgct ttcggtgccg aaacaccttt cgccgggtta
     5041 gctttgagtt cgttctggct gaccagccag tcaaaaaagc tacgtagcgc agaaagccgt
     5101 aacgccagac ttgctgctcc cagcccttta cggcgactgc gtacagcaaa attgcgcacc
     5161 atcgtcacat cacattgctg ccagctttgc aggccgtttt cgctggcaaa attgatgatc
     5221 gcctcaagct gacgctggta gttaagcagg gttatcgggc taagctggcg ctccacgctc
     5281 agataacgta ggtagcgttc tacatcggtg tgtaaatcgg tcatacgcgt tcaatccaac
     5341 gctccagaag ctccggcaac atcagcgcaa tttcatgaag taactgcgtt ccttgccctt
     5401 gttgatagtg actggcatcg cgactggtaa acagcacgac acccaaatca gcatcgcttc
     5461 ccagcatcga catcgccacc gatcccaccg ctttcgcttc cggtagcacc accagcagct
     5521 ctggtccgtt aagcggccca agatagtgct gttcctgccc caaacgctga atacgcagcg
     5581 gttcgaaaga ctgacggctt aatgccagat gagtgtggtt cgacggcgca cctaagcgcc
     5641 agcgatccgg aaacaggcgc agactcgcac ctgccaggcc gagatcgcgc gcccagcggt
     5701 gaaagcgcat cagcatatcg tcgagactgc tggcggcggt gagactgcgc tgcaggtaga
     5761 gtaggcgata aaacaggcct tcgttggcga tagcctgttc catcaacagc gccatgttct
     5821 cttccagaac atgaatatga ttacgtgcgc gggccatgtg ccactcgacc aacgaaacgg
     5881 tgccgcgtac cggatgtggc acacgtatcg cttctactgc gcgcgcatta cggataaaaa
     5941 actcaggatt tttaatcaga taatcgacaa ccgcccggtc atcaagctcc gtgagtgttt
     6001 cctgcagttc ttcccctggt tgcttcatag atgaataaat ccgtcgtaga catgtaccgc
     6061 cgggccagtc atatataacg ggtgacccgg acctttccag gcgatatcaa gacgaccgcc
     6121 ggggagttcc acgcgtactt cttcggccag caaaccttgc tgaatcccta ctgcaaccgc
     6181 cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc ccacgctcat aaacgcgtaa
     6241 acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata ttggcgcgct ccggaaaacg
     6301 ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc gcggtatcga catcatcgac
     6361 ctgaatcacg caatgcggat ttcccatcga caccacgccg cataagattg tctgctcggc
     6421 ggcgcgcata atataggtct tttccgcttt gttagcgcga aacggcacag cggaaggttc
     6481 gaagttgggt tcgcccatat ttacgcggac cagatcatca tcggtgacgg tcagaaccat
     6541 ccgcccgttg gcggtgctga cgcggatatc gcgcttattg gtcagccctt tcaggcgcac
     6601 aaaacgggca aagcagcgcg caccgttgcc gcactgcgcc acttcactgc catcagcatt
     6661 gaaaatgcga tagtgaaaat ccagttcagg atcatacggc ggctcaacca ccagcagctg
     6721 gtcaaaccct acccccaggt gccgatccgc cagccgacga atcagctccg gtgaaaaaaa
     6781 gacattctgc gttaccgcgt cgacgaccat aaaatcgttg ccaaggccat gcattttcga
     6841 gaactgcatc atttactcca atcacgcggg tacagaaact gacttttagt aattcacctg
     6901 ggatggacca tcccagtggc gcgatcgttt ttatccggca ccgtggattg cgtttgcgtc
     6961 tctaccggtt tggtcggcgg cggtgcattt ttatctgcag gcgggaaata gagcggacct
     7021 ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtac cttaaacacg
     7081 tttttcatta tggattgcct gtaaagttca tcgctttctg ctttctatca tcgcaggaga
     7141 aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct gaaaagtgag gaaatgctgc
     7201 gagtataacg cttactcgtg aggatttatg ttctgctggc agtttcgtga tcggcacctc
     7261 gcgccaggat ggctttatgc actatcgcaa tgtggtttgt ttgttggctt ttttagttcc
     7321 gtcagcgtga ggatgctcgc tcggtaaacc tgatcataat gtttaccaga aacagggaaa
     7381 ggcgtggtgt aactgcgccc taatgaagag acaaatcttt cgccacacag agacatcatt
     7441 attccggtga cggatgaatc atgccggaac gcagcagcag aggtgcaaac tttgaagccc
     7501 tgaccccatg ccgctatact gccgccatca cttaaaaaca ggatacaaca atgaacgaca
     7561 gtgaatttca tcgcctggct gatcaactgt ggctgaccat tgaagagcat ctggacgact
     7621 gggatggcga cagcgatatc gactgcgaaa tcaacggcgg cgtgctgacc attacctttg
     7681 aaaacggcag caaaatcatt atcaaccgcc aggaaccgct gcaccaggta tggctggcaa
     7741 ccaaacaagg cggctaccat tttgatctga aaggcgatga gtggatttgc gatcgcagcg
     7801 gcgaaacctt ctgggatttg ctggaacagg cggcgacgca gcaggcgggt gaaacagtca
     7861 gtttccgcta agattgcatg ccggataagc ctcgctttcc ggcacgttca tcacgaaaaa
     7921 tattgctgta atagcggcgt atcgtgatcc tgattggcag gcggcatgtt accgatagat
     7981 tttgtgcgga acggaatcac ctgttcacga ccatcaacct tcacaatctg atagaactgc
     8041 ggcaggttga agttgatgaa gcttgagccg taggtgaagc ggtcgtgcga tgacgagtag
     8101 aagcgactga cgtcacgcac cagttcctct ttgctgcctt cgcagtggtg atacacctca
     8161 acccggttgc tttcgtcaag aatgtagata ttaaagccat tctcgtcttg cgtttcttcg
     8221 aagaagaact ggatgatccc ttcactagca aagccgtcca ccaccgccgg taatttgacg
     8281 tgattggttt caacctgcac tgacaggccg tgcagtttgt tatgcgaaat agcgccataa
     8341 aactcgatgg cgttctccag cttctgtacc gatacattca ggcgttcgaa gaacaagccc
     8401 caggtttgac cagaaacgcg cagcgccttg aaacgcccgg tttcctggcg ggtgctggaa
     8461 aggcgcagtt caatacactc agaaaccagt tgctgcacgc gagtacggat taacccgcac
     8521 agatgctggc tataacagaa gacttccacg ctatctggcg gtgcggcgtc ctgatgcatt
     8581 ttgccgagaa tagttttcag ggcttcgatc atcgattgct cgccgttgaa gtgcagcgta
     8641 cgcacttcgt tccacgagtt gcggtacagc aggtcaacgc tacctaccag gcaattttga
     8701 ttctcgccaa agctgaagac atccagctta cggaaatcaa aatgcaccac ctgattgcgg
     8761 aacgccgctg tcgggtcata ttccaggtta acgataatcg ccagatggcg gatctcacac
     8821 gggctgtaga gcgccttcgg tgtcggtgca ggtaagcgca gcgggaaatg gtgcgacacg
     8881 tcggcgacca tctcctgcaa cttaggcaaa tcgacaatgc cgttaccttt aatatacaaa
     8941 cgggtgcgcg aggtcagcag gccgttaaac catgcccacg ccaccagttt gttcaggtaa
     9001 cggttatatt ccagcggctg atggctgatg atcgactcaa tatttggcgc gcggttatac
     9061 agataccaac ctgaacggtt agcacggccc ggcggcacat aaataaaggt cagattcggt
     9121 tccgagagat cgggtgaaat ctgcgggttt accagcgtca ctttacctgg taatgcttca
     9181 aacgcggcat acagcttacg ggtcagcacg ccgatatcct gcggactggc ggagacgcta
     9241 aggttattgc gacgcgcaaa gcggatcaga ttacggtagc tctgcatcat cgcgtcgagc
     9301 aactcgttgt gcgcctcacg cacctgatca atcttccagt tagcgcggtt atcgagcatt
     9361 gccagacgag cttcgtccca accccactcg ctcactaact ggctcaacac tgcgcgacgc
     9421 cagcctacgc aggcgcgttc acggctgagc ttttcgcaca cttttaaata gaagcagcga
     9481 cgtactaaat ccagacgggt aaaatcttca atcgccgtca ggtattcagt aacacgctcc
     9541 agcatcatgc agtatggatc gagaccaaac gatacaatct caccgtcgtg caaacgctgt
     9601 ttgatatctt tcgccagcag acgtgggttc gggtattccc aggaataggc ttccagcagc
     9661 agtgttttca gtaccgcttt gtatggggaa tcgatactct tgtagagctg ccaaaggctg
     9721 gcaccaaagt actcttcagc agaaagcgag cttaagccac cgagatccag ccattcattt
     9781 ggcgtcagca cgccctgcgc gtaaagcgtc atcacatagt cgtcgtaatg ctcttcttcg
     9841 tcgcacggca ccatattcca cagaatacgc ttaccggcga gacgcacggc ggtacgataa
     9901 aattcgtcaa gcagcagtat atgctgggtg gagccacaat cttcgccccc caagctgccg
     9961 ctttcattat gacggaagcg gttttcatca atcaggaaga agctgacttc cacacccagc
    10021 gaggcggccc agctttccag cagactacat ttacgttgta gtaactggcg ctcttcgcta
    10081 tcgagccagg attgatgaca gacccagata tccaggtcag aggaacaact ttgccctacg
    10141 gacgaggtgc tgcccatggt gtatacacca gtaattggaa gctcaccttt cggtggatcc
    10201 tgtactgaca ttccacgata cagttcaagc tcgttcaggt agtggcgttg agtttcatca
    10261 ggcgtgtaaa ggcaaatgcc tttgggaacg ttaccatcaa ggtaacccgg cattagcgga
    10321 tggtgatagt gcaacaatgt cggcagtaga ctgtagacct gttggaatgc aggccccata
    10381 gcagcaagcg cgcgatccac acgcaattga tttatggcat ccagtctctg tttcagagtc
    10441 tcaatataga ggtacaagac gtatcgcctg atttgctacc cgtcatgact gtgattccgc
    10501 caacatcaac ggtaacacgc ggcattcggg atatttcgta tgtcaaaggt aaccgttacc
    10561 acttttcgcg cctggttttt ttagtttcac gacgaaaaaa tggtctaaaa cgtgatcaat
    10621 ttaacacctt gctgattgac cgtaaagaaa gatgcgctac atacaagtgt agcaccgttt
    10681 attctctgta aattccttat tacgacggcg tgaaacgcct gtcaggatcc actgccagac
    10741 ctcattttac ggtttgcgca ggcgtctacg tttcaccaca acactgacat cactctggca
    10801 aggatgttag gatggaccac ggatgataat gacggtaaca agcatgttag acaatgtttt
    10861 aagaattgcc acacgccaaa gcccacttgc actctggcag gcacactatg tcaaagacaa
    10921 gttgatggcg agccatccgg gcctggtcgt tgaactggta ccgatggtga cgcgcggcga
    10981 tgtgattctt gatacgccgc tggcgaaagt aggcggaaaa ggcttatttg taaaagagct
    11041 ggaagtcgcg ctcctcgaaa atcgcgccga tatcgccgta cattcaatga aagatgtgcc
    11101 ggttgaattc ccgcaaggtc tgggactggt cactatttgt gagcgtgaag atcctcgcga
    11161 tgcctttgtg tccaataact atgacagtct ggatgcgtta ccagcaggca gtatcgtcgg
    11221 gacgtccagt ttacgtcgcc agtgccaact ggctgaacgc cgcccggatc tgattatccg
    11281 ctccctgcgc ggcaacgtcg gcactcgcct gagcaaacta gataacggcg aatacgatgc
    11341 catcattctt gccgtagccg gactaaaacg tttaggtctg gagtcccgca ttcgcgccgc
    11401 gttaccaccc gagatttctc ttccagcggt aggacaaggt gcggtcggta ttgaatgccg
    11461 ccttgatgat gcacgcactc gcgagctgct tgccgcgctg aatcaccacg aaactgcact
    11521 gcgcgttacc gcagaacgcg ccatgaatac ccgtctcgaa ggcggatgtc aggtgccaat
    11581 tggtagctac gccgagctta ttgatggtga aatctggctg cgtgcgctgg tcggcgcgcc
    11641 ggacggttcg cagattattc gcggtgaacg ccgcggtgcg ccgcaagatg ccgaacaaat
    11701 ggggatttcg ctggcagaag agctactgaa taacggcgcg cgcgagatcc tcgctgaagt
    11761 ctataacgga gacgccccgg catgagtatc ctggtcaccc gcccgtctcc cgctggagaa
    11821 gagttagtga gccgtctgcg cacactgggg caggtggcct ggcattttcc gctgattgag
    11881 ttttctccgg gtcgacaatt accgcaactt gctgatcaac tggcggcgct gggggagagc
    11941 gatctgttgt ttgccctctc gcaacacgcg gttgcttttg cccaatcaca gctgcatcag
    12001 caagatcgta aatggccccg actacctgat tatttcgcca ttggacgcac caccgcactg
    12061 gcgctacata ccgtaagcgg acagaagatt ctctacccgc aggatcggga aatcagcgaa
    12121 gtcttgctac aattacctga attacaaaat attgcgggca aacgtgcgct gatattacgt
    12181 ggcaatggcg gtcgtgagct aattggggat accctgacag cgcgcggtgc tgaggtcact
    12241 ttttgtgaat gttatcaacg atgcgcaatc cattacgatg gtgcagaaga agcgatgcgc
    12301 tggcaatccc gcgaggtgac gatggtcgtt gttaccagcg gtgaaatgtt gcagcaactc
    12361 tggtcgctga tcccacaatg gtatcgtgag cactggttac tacgctgtcg actattggtc
    12421 gtcagtgagc gtttggcgaa actcgcccgg gaactgggct ggcaagacat taaggtcgcc
    12481 gataacgctg acaacgatgc gcttttacgg gcattacaat aactctcata acaggaagcc
    12541 ataatgacgg aacaagaaaa aacctccgcc gtggttgaag agaccaggga ggccgtggac
    12601 accacgtcac aacctgtcgc aacagaaaaa aagagtaaga acaataccgc attgattctc
    12661 agcgcggtgg ctatcgctat tgctctggcg gcgggcgtcg gtttgtatgg ctggggtaaa
    12721 caacaggccg tcaatcagac cgccaccagc gatgccctgg ctaaccaact gacggcattg
    12781 caaaaagccc aggagagcca aaaagccgag ctggaaggca ttattaagca acaagctgca
    12841 cagcttaagc aggcgaatcg tcagcaagaa acgctggcaa aacagttgga tgaagtccaa
    12901 caaaaggtcg ccaccatttc cggcagcgat gctaaaacct ggctgctggc tcaggccgat
    12961 tttctggtga aactcgccgg acggaagctg tggagcgatc aggacgtcac gaccgctgca
    13021 gcgttgctga aaagtgcaga tgccagcctg gcggatatga atgacccgag tctgattacc
    13081 gttcgtcggg caattaccga tgatatcgcc agcctttctg cagtttcgca ggtggattat
    13141 gacggcatca tccttaagct taatcagctt tcaaatcagg tagataacct gcgtctggcc
    13201 gataatgaca gcgatggttc gccgatggat tccgatggtg aagagctttc cagttccatc
    13261 agcgaatggc gtatcaatct gcaaaaaagc tggcagaact ttatggacaa cttcattacg
    13321 attcgccgtc gtgatgacac cgccgtaccg ctgttagcgc caaatcagga tatctatctg
    13381 cgcgaaaata ttcgctctcg cctgctggtc gcagcacaag ctgtaccgcg tcaccaggaa
    13441 gagacttatc gccaggcgct ggagaacgtc tccacctggg tacgtgctta ctacgatact
    13501 gatgatgcca ccaccaaagc gttcctcgac gaggtggacc agttaagcca gcaaaatatc
    13561 tcgatggatc ttccggaaac cctgcaaagc caggcgatgc tggaaaaact gatgcagact
    13621 cgcgtgcgta acctgctggc acaaccggca gcagggacaa cggaagctaa acctgcacct
    13681 gcacctgcac ctgcacctgc acctgcacct gcacctgcac ctgcacctgc acctgcacct
    13741 gcaccgcaag gtgatactcc ggcagccgcg ccgcaaggag aataaccatg ctaaaagtgt
    13801 tattactctt tgttttgctg attgcgggga tagtggttgg cccgatgatt gccggtcatc
    13861 agggttatgt gctgatccag accgacaact acaatatcga aaccagcgtc acgggcctgg
    13921 cgatcatatt gattctggcg atggtagtgc tgtttgccat tgagtggcta ctgcggcgga
    13981 tcttccgcac tggcgcgcac acccgtgggt ggtttgtcgg gcgtaagcgt cgccgtgcac
    14041 gtaagcagac cgaacaggcg ctgctgaaac tggcggaagg cgattatcag caagttgaaa
    14101 agctgatggc gaaaaatgcc gatcacgctg aacaaccggt ggtgaactat ctactggctg
    14161 ccgaagccgc gcaacaacgt ggtgatgaag cgcgcgccaa ccaacatctg gaacgcgcag
    14221 cggagctggc cggcaatgac accattccgg tagaaatcac ccgtgtacgt ctgcaactgg
    14281 cccgtaatga aaaccatgct gcacgccacg gcgtggataa gctgctggaa gttacgccac
    14341 gccatccgga agtattacgt ctggcggaac aggcgtatat ccgcacaggt gcatggagtt
    14401 ctctgctgga tatcatccca tcaatggcga aagcccatgt tggtgatgaa gaacatcgtg
    14461 caatgctgga acaacaggca tggattggcc tgatggatca ggcgcgtgcc gataacggta
    14521 gtgaaggttt gcgtaactgg tggaaaaacc aaagccggaa aacgcgtcat caagtagcgt
    14581 tgcaggtggc aatggcggaa catcttattg aatgtgacga tcatgatact gcccagcaaa
    14641 ttatcatcga tggcctgaaa cgccagtacg acgatcgcct actgctgccg attcctcgac
    14701 tgaaaaccaa caatccggaa cagctggaaa aagtgctgcg ccagcagatc aaaaacgtcg
    14761 gcgatcgccc gctgttgtgg agcacactgg gccagtcact gatgaagcac ggagaatggc
    14821 aggaagcatc gctcgccttc cgcgcagcgc tgaaacaacg tccggacgcc tacgattacg
    14881 catggcttgc cgacgcgctg gacagactgc acaagccgga agaagctgca gcgatgcgtc
    14941 gcgacggttt gatgttaacg ttgcagaaca acccaccaca gtagttcctt ctcacccgga
    15001 ggcaagcacc tccgggacct ttctgatgca taaaaaaacg cctgctctta ttacggagca
    15061 ggcgttaaaa caggtctgta tgacaacaag tgggtgcttc actcaacgtt gtgtccatgg
    15121 tgtctgatga ggcataagcg acatctgtca gtggacgata agcaccgtaa acggctctgc
    15181 gtcattccgg agtttatgag gcactaaggc gaacataaga gatggaatga gcatctactc
    15241 gtttattatg ccacagagaa tcgggaaata acatccctta acacttgtta tgagataatt
    15301 ctgtaatcct ctttgcttcc tgagtaataa cttcctgagt gaatatttaa cctgagcttg
    15361 atcctacaca tacttattat gaatgataaa attcattcaa ttaataacac atatattaat
    15421 tgccgttaaa actaaaaaaa gcatcaataa tcaacgcgat ataataaacc tgccttacat
    15481 atcaactgcg ccagaggtag gattgaaaac gctctcctga ttttccaatt cattttctgg
    15541 ataaataaat aatttatttt tgtcactatt atttatgtaa tcatcctgtc agggagaggg
    15601 atctcaatta tcaatgctta attacgtcat cattttgatg acatgaaacc ggagcatttt
    15661 gcatggaatt ttcgttttca cccaaacttc ttgttgttgc tgtcgccgcc gctcttcctc
    15721 tcatggccag cgcagcagat accccgtcaa ctgccaccgc acgcaaaggc tttgccggat
    15781 acgatcaccc aaaccagtat ccacagaacg ctgcaattta ttgaatttgc acgatcatgt
    15841 aggccggata aggcgtttac gccgcatccg gcaatcaacc gcaggcggcc acaaaatggc
    15901 cgctatttta cttattcacc accagcaaat gtgcctgcat aatgtcactg gccgggcgac
    15961 cgtgcgccag caaatcagcc attgctttaa gatatggcgg tagatggcgg aaataacgct
    16021 gatacccggc acacaaataa ttcagccccg gtttgccgct ggcatcgagc atgaagcggt
    16081 gtttcgggca gcctccccag cacgctttta acacgttaca actgcgacac tgcgccggta
    16141 attgcttaaa tttatcttca ccaaacacct gctgttgcgg ggaatcgatc atttctgcaa
    16201 ttgtttgctg atgcatattc cccagccgat attgcggata aacatagtga tcgcaggcgt
    16261 aaacgtcgcc gttgtgctca acaatcaccg agcgcccaca ggttggctga tgatggcaaa
    16321 ccgcacccgg cgcaccgaca aaattggcaa acgcccattc gatattcatc acgaaaatct
    16381 tgccgacgtc gcgtttgatc cagtggtcaa aaatcgccac cagaaattca ccaaattcat
    16441 cggggcgcac agaccattcc gtcagttccc cctgaatatc accaggcgca tgtagtttca
    16501 gtccatcgct ggcagctgtt tcatcagcta ggcgctcgac caccggaata aactggatga
    16561 attcgactcc cgcatcgcac aaaaaatcat aaacctgcaa cggttgctgc gcgctggtgc
    16621 ggttgacgca gaccagcacg ttatagtcga catgatgttt ttgcagcagc gtcagggcac
    16681 gcatcaccag cttatgcgtt ggtctgccac ctttggtcac gcgatattga ttgtggatct
    16741 cagccggacc gtccagcgat aacccaacaa gaaaatgatt ttctgccaga aatgcacacc
    16801 atttatcatc gagcagcacg ccgttagtct ggaagctgtt acttatcttc ctgccagcac
    16861 cgtatttcgc ctgtaacttt acggcacggc ggtaaaaatc cagcccgagt aacgttggtt
    16921 cgccgccctg ccaggtaaaa gccacttcgt tttgcggttc gctggcagcg atatagtgac
    16981 ggacatacgc ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg cggtaaaggg
    17041 attgtttttc gagataaaaa cagtagtcac agttcagatt acaatcggaa ccactcggtt
    17101 tcgccatcac atgaaaagca cgcgttggaa cctgttgcag catggtcact ccttgcctga
    17161 agtcatcctt caatacgtta agggcgaaat cataccgtgg ggcaatgagt tattacgggc
    17221 gtttattgcg aagagagggt gacgaaatgc acagaaaaca aaaaaccccg ccgaagcggg
    17281 gttcaaaatt ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt
    17341 gcgctaccaa gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattatt
    17401 taaagtcgtg gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct
    17461 gaagctagcg cgtctaccaa ttccgccacc ttcgcattgt cacaacttct aataatgggg
    17521 tggctaatgg gattcgaacc cacgacaact ggaatcacaa tccagggctc taccaactga
    17581 gctatagcca ccactacaaa tcttgttacg cggtattact accaccgcag ctcaagcgcc
    17641 gggactaaat ggcgcgcccg acaggattcg aacctgagac ctctgcctcc ggagggcagc
    17701 gctctatcca gctgagctac gggcgcttag cgccgttgcg ggggtggata atacggactt
    17761 cccacccctc tgtctagtcc ctttttaaat aaattgcgcg tttggttacg gtttgtgcgt
    17821 tttgctgctt attcctccag tttatgcgct ttcccgtggc gattaaggcc aaaaacttta
    17881 taaatcgccg tcaccgccag cataaagatg atgccaacaa acagcgacat acgcgtatct
    17941 tcattaaagt acatgccgat caaaacgcaa atcaggaatg ccattgttac gtagttggcc
    18001 cacgggaaca ggattgagcg gaacggatgg ctggcaatcg ccgctttatg tgcacgccga
    18061 aaacgcagct ggcttatcaa tatcacaaac catggcacca tccccggaag cacgctggca
    18121 ctgtagacgt agacaaacac acgctgcgga ttgggaatga tgtagttcag gcatgagcca
    18181 attagcagaa tagcaataga tactgccaca cccgcaactg gtacgccgtg acgggaaact
    18241 ttcgccatcg ccgccggtaa ctgacggttt ttcgccagtg cgtagagcat acgtccgcaa
    18301 ctgtacatgc cgctgttaca gccagagagc gcagccgtca gcaccacaaa gttgataatg
    18361 cccgccgctg cggtaatacc gattttggca aaagtcagta cgaacgggct gccgttgctg
    18421 ccactttcat tccacgggaa gatggtgacg ataacgaaaa tcgcgcctac gtagaaaatc
    18481 aggatccgcc acagcacttt gcctacggca ctgcgcaacg tcacctgcgg attcttcgct
    18541 tcaccggcag taatgccaat cagctccacg ccctggtagg acgccaccac aatacacagc
    18601 gcggtcagga accctttcca gccacccgca aagaaaccgc catgctctgt gagattgcta
    18661 aaaccaatcg actgcccgcc attgccaaag ccaaagaaaa tcacgcccag gccaatgaca
    18721 atcatcacga taatcgtggt gactttgatc atcgcgaacc agaactcgat ttcgccgtac
    18781 aaccgcaccg ccgccagatt cgccaacgcc accagcgcca ctgcgatcaa tgcgggtatc
    18841 cactgcgcca tctccgggaa ccagaactgg acataaacgc caacggcggt gatttctgag
    18901 atccccaccg ccatccacat aaaccaataa gaccaggcgg tgagataacc aaagaacggg
    18961 ctcatataac gatgcgcata aacggcgaac gaaccggtaa ccggttcgag gaacaacatt
    19021 tcgcccattg aacgcatgat gaaaaagacg aatagcccgg cgatgatata ggccaacaat
    19081 acggatggcc cggcccattt caaggtactg gcggccccca taaacaggcc gacgccaatg
    19141 gtgcccccca gggcgatgag ttcgatatgt cgagcttcca gcccacgctg tagctctggt
    19201 ttgttatctg ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt accggttatc
    19261 gttatgggta catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg gcaaataaag
    19321 cattaaaaat ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga gtgaccactc
    19381 cgtcgcttta caaagagagg aaaatcagag gttgccggtg tagtgccagc gtaaataacg
    19441 cagcaaacga agctgacgct taatgcggct cggctgcgaa agcaggcggt agagccactc
    19501 cagtcccagc gtttgccaga ttttcggtgc gcgttttacg tgaccggtga aaacatcgta
    19561 agtcccgcca acgcccatat acagcgcatc tggatgcacc agacggcagt cgcgcatgaa
    19621 gatctcctgc tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc tggcatgaat
    19681 gcgttcaaac agcgcctgac gctgctcggg tttaaaataa ccatcctgac tgccaacgat
    19741 attcacattc cactggttgc gcagtttagc ttcagtttgc gccagcactt caggtttacc
    19801 gcccacaaga aataccggcg tcccttcttt gcctgcgcgc gccatcagct cttcccagag
    19861 atcggcaccg gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta ctgaacgtac
    19921 aacgctgatg ccatccgcat atttaaattc ggcagcgtta attaactccc tgacctcggc
    19981 gttatcttca atggtcagca ttttttcagc attaatggca accagcgttc cctgcttaag
    20041 ctgcccgtca gcaaacagat aatcgagggc gtgctgcata tcacgccaac caatcaactg
    20101 taagccacgc agcgtatagg tcggtgccgt ggtgttgtta ttcattgtta tccttcaacc
    20161 tgcgtccgga gcgatgattt tgtacgttta tgaatgagtc cggcgctttc aaaaagccag
    20221 tacaacagtt ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa gactacgcgt
    20281 gagacaaacg aatccagccc ttcacgtgcc agcacgatca tattgaagat cgccccaaag
    20341 cagaaactgt gcaatatcgc agccttatag cgattaggct cgcggttgcc cagctcatac
    20401 agccagtcga accatttgat gatcagacca accacgatcg ccccgagcgg gatgaacaac
    20461 gcgccgccca tcaccaccag tgagcctata agcgtaggcg agatcgccag tccggagtgg
    20521 ttattcagca cttccctggt aaagtagttg gctgagttca gcaccatact cgggcgaccc
    20581 ggccacagcc aggaagggat aaagacatag aaatcgcgga caattggagc caggccctgg
    20641 aagtcgatgt tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg cgagaaggtg
    20701 tcgcgagtga gatagagaaa cgtatagaac gcttcatcgc cgctcacatt cattccatag
    20761 cgttttagcg ccagccagaa catgcccaca atccccagca cgcccgccgc cgccagcatc
    20821 cacaacgaaa tccagccgcg aataatgcca ataaacagga agatagcgaa tgcgatgatg
    20881 atattggcgc gagtgccgcc gacaatcata taagtcagca agccaaaggc gaccgtgctg
    20941 acgaggaaaa acagccacgc tttgctatcc tggcgcagaa agtagaccac cagcatcgcc
    21001 gggatgaaaa agtaaaagaa gcgttttaac gccacgccgg agacttcact ggagaagatc
    21061 tgactgtagg agttaagccg gaacagcaaa aagccgttgt gcatgaagaa gatgccgacg
    21121 cttaccagcg cgatgcccat caggatcacc cacgtaagat tggtctccac gcggttcatg
    21181 gtaaacagcg gacggcgcgg tacatcagca acgcgtttgc gtaggcgggt tttgtaggtg
    21241 acatagtaaa ccgcgtagaa gcagcccgca gaaagcaacg cctgcaacaa gatttctggc
    21301 ggcgcgacac caacatcaaa gcgaaatacc agcacgctgg tcagcgggaa gccgaagaaa
    21361 aaggtgagca aaaacagcaa tgaaaagaag acattgaagt taaagcgcac acggcgaaac
    21421 tcaaaccaag tcagcgtggc aataaacagc gtgcagagca gccagaccac aaacaggcca
    21481 ctgaattgca gcagactcat gcgacctccc cggcggcaat cgccaacacc cgctgccagc
    21541 cttgtagata gttagggcta aagaaggcaa tggtgttttt atccaccgac gccaactggc
    21601 gctgcgcttc acgcacaata tcctcgttga gatcgtcggt agtaaacagc accggcaaat
    21661 gctgttccgt catatcctgc cagaacggat tttcccggtt aagcacacaa ggaatgcccg
    21721 cctgaatcag taagcaaagc gtaccaatgc cctgctggcg ggcaaaaata aagtaaccaa
    21781 gatcgcactg acgaagtagc gccagatagg cgtcaaattc cagtttttcg ctcaaaattt
    21841 gcagattttc ttcgctgaat aactccagcc ccgcctgacg aacttcctca atgtacgctt
    21901 cgttattagg cggatatccc atcggcacca ccacttttac cgtatcgcca aattgctgat
    21961 gaacggcgcg caaggcagca atatggtcat tgctgcggtc accggagttc cccaccagaa
    22021 tggtcatttt cccttcacgt tgccgatcgt tcgccatcgt attgagagaa gggtccatcc
    22081 gcgtcgggaa gtacagcagt tcgccccgca cctttggatg cgttttggca aaaaagctca
    22141 aatcaccgcg ggtggcaaat acacagccga ctcgcttttg cgccaggcga cgtagcgggt
    22201 aaaaaagctt atatctcaag ccactggaaa gctcatacag gtctgccccc cagatatgcc
    22261 agaaaaactg gctgggctta atgccaccac tcagcagagc cagccacagt gtgggattga
    22321 actgaccgtg gaaaaaaaat cgctgctgac ggttagcttt tgctttcgcg atgaccgctt
    22381 ccgccagcga ttttttccca gggaaaaatt gcacagaaag cgccggacag ctatcactta
    22441 agccgtcgtc cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc gcggccagcg
    22501 cgtcattgaa aaaccgcaaa acggttcggt tatggtgagg gatatccgat cccagtacgt
    22561 gaatcagtac agtcatgccc gcctacgcca gagtaaaaac acgccacaac aaagagaaaa
    22621 atagacgata taagttgcca tatatgcctg cgccgcgccc agtgcaccat gcgcagggat
    22681 tagccagtgg gcaaatacca tcaataaagt gaactggctg acttccgcca gaatataaaa
    22741 ccgcagtgac gctttggcga tcaccagata accaaagaca taagcgccca cttttagcac
    22801 atcacccact aactgccagg caaagagatc gcgcatagcg gtaaatttat tcgacaacag
    22861 caaccagata gcaaaatcac gcagcaacca gacggtaaaa ctcgccgccg ccactgccgg
    22921 taagacgaat tttagcgatt taaccacttc ccgggtgata tcgcgctttt ccgttagccg
    22981 cgacaacgtg ggcagcaaat atacgctgaa cgatgccgta atgaattgca ggtaggcatc
    23041 ggaaatactg ctcacccctt gccagatccc cacttcatcc cagctatact gcgccgccag
    23101 cagtttacgc atcatgatgt aagcaacagg caaggtcacc gacgtaatca acgccatgag
    23161 cgtaaatttg ctcaactgcc ctgccagacc gttatcccag ctgggtttca gatagcttaa
    23221 cgggatgaca ccacgtttga tcaacatgat ggcggcagga attaccacca gcgcgggaat
    23281 cagcgccaga cccagcaacg ccccttcata accgcccaaa cggtaactga cgtagtacgc
    23341 gagaacgcca atcaagctgc cgacaatcag cgataacgca ttacctgcgg catcgcgaaa
    23401 gcctttcatc agcgccagta acaggttgcc ccaggcgatc cccatttgca ccagcgccac
    23461 taaacgcacc agcccctgat agtcggtatt accaaacaat ccctggctga ttggcgcagc
    23521 tgccagcaca aaaaccagcg ccatcagcgt agagaaacca agtaccatcg ctgatgaagt
    23581 gccgaccacg cggcgcagct gttgcggatt atcatggtac tgggcaacgt atttggttac
    23641 accgttaaag atgccagccc cggcaagcac gccgagcacg gtaatcaact ggcggaaatt
    23701 tgccgccagc ccaagccccg ccggaccaaa tgacaccgcc agcaacttac cgaccagtaa
    23761 cccggcacca atcttgacca gtgtactggc cgccgtccac aaggacgctt ttgccaacga
    23821 catatcagga gaagtagttc aacaaagtcg caattaccgt acgctgattg acgggcgaca
    23881 ggttgtagaa cagcggcagg cgcagcaggc gctcgctctc tttggtggtg tagcgatctt
    23941 caccgtggaa ctcaccaaag tgttcccccg cagggcaacc gtgcagcgga atgtaatgaa
    24001 acaccgccat gatttccgct tctttcagaa agttaatcaa cgcgctccgg tcatcaatat
    24061 cccgcagttt aatgtagaac atatgcgcgt tctgcacgca gccatcggga atcgacggca
    24121 gctcgatacg cccggctttc gccagaggcg ctaacgcatc gtagtaattt tgccacagcg
    24181 ccagacgttg ctggttgata cgatccgctg cttccagttg cgcccacagg tatgcagctt
    24241 gcagatcgga catcaaatag ctggagccaa tatcgcgcca ggtatattta tcgacctgac
    24301 cacggaagaa ctggctgcgg ttagtgccct tttcacggat gatctcggct cgttcgatta
    24361 acgctttatc gttaatcagc gtcgcgccgc cttcaccacc cgccgtgtag tttttggttt
    24421 catggaagct aaagcagcca atatgaccaa tggttcccag tgcacgccct ttgtaagtgg
    24481 acatcacgcc ctgagcggca tcttccacca caaacaaatt atgctttttc gccaacgcca
    24541 taatggtgtc catttcgcag gccacacccg cgtaatggac cggcacgata acgcgcgttt
    24601 tgtcggtgat cgctgcttca atcaacgttt cgtcgatgtt catggtgtcc gggcgaacat
    24661 ccacaaagac gattttcgcg ccacgcagca caaaggcatt ggcggtggag acaaaggtgt
    24721 agctcggcat gatcacttca tcgccaggct ggatatcgag cagcagcgcc gccatctcca
    24781 gcgaagcggt gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt tgctccagcc
    24841 actgctggca gcgacgggta aaaccgccat cgccacacag tttgccgcta cccattgccg
    24901 actgcatata gtcgagttcg gttcccacca ccggcggtgc gttaaatgga atcatgtgat
    24961 cacctgtata accagtacgc ggtgctttct acattcgcac cactttgtat gtatcgttta
    25021 agcgcggcgg tgttgcccat ttgggtcgcc acccgcaaag ttgttttacc gcgagcatac
    25081 gcccagttta gcgccgtttg catcagctca gcacctgcac cgcgtccagc cagcaggcca
    25141 attcgcgcat ctgtcgcatt gagttcccgt aaagagacat agccgcgaat atcgccggac
    25201 gccgcacgta gaatcagaca ttgatgatca aaggtgccgc gcacggcatt ttcaatccac
    25261 tgtgcataaa agcgaccgct ggcgtcaggc gcataccacg gcgcacgaaa acggctttgc
    25321 gcaaatgcgg cgctagctaa ctgacgtaat gcgggaatat cggtctcttg tgccacgaca
    25381 gcaccgctat cactgacatt gttcacgggt agcgccaaat caacttcacc ttctaccagg
    25441 gagaatccca gctgttgcag ggcatccagt tcacccgtat ttgatgccgc aattttggcc
    25501 tgcacccgtg accacggcgc taacgcgtct ggcgtcagga gcggtgcttc agacgtaatg
    25561 cgcacgatgg cgctgttaac accaaagaag gcgttttccc aggttagtgg ctcaatactg
    25621 gcgcggacgg gcacgaagta actccagcag atattggccg tagccagttt tcgctaatga
    25681 actggcagca cgcttcacac cctcgtcatc gagccagccg ttacgccagg cgatctcttc
    25741 caggcaggca atcttaaagc cctggcgttt ttctactgtc tgcacaaagg tactggcttc
    25801 aatcaggctg tcgtgagtgc cagtgtccag ccaggcaaat ccgcgcccga gcagttcaac
    25861 ggtcaggttg cccgcctcga ggtacatctg gttgatggag gtaatctcca gttcaccacg
    25921 ctccgacggc ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga aataaagccc
    25981 ggtcaccgcc cagtttgact tcggctgttt tggcttttct tccagcgaga tagcgcggaa
    26041 attgtcgtca aactccacca cgccaaagcg ttcctggtcc atcacctgat agccaaatac
    26101 cgtcgcccct tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc cctgaccgaa
    26161 gaagatgtta tcgcccagca ccagacaaga aggttcaccg ttgaggaagg tttcaccgat
    26221 gataaaggcc tgcaccagac cgtccgggct gggctgttcg gcgtattcca gctggatacc
    26281 gaactcacta ccatcgccca gcaggcgctg gaaataacct ttatcttccg gcgtagtgat
    26341 gatgagaatt tcgcggatac cggccaacat cagcaccgac agcggatagt aaatcattgg
    26401 cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc gtaatcggat gcaatcgggt
    26461 gccggaaccg cccgccagga taataccttt catgtttgcc tccgctgaaa attagccttt
    26521 cagacctaaa cgctcgccct gatagctgcc gtcctgcacc tgcttccacc agctttcatt
    26581 agccagatac cactgcaccg ttttacgcat tccactttca aaggtttcct gtggcagcca
    26641 gccaagttca cgggcaattt tcgaagcatc aatggcataa cgcagatcat gccccggacg
    26701 gtcagcgaca aaggtgatca agtcacgata gtgcaccacg ccgtgcggct tgttcggagc
    26761 cagttcttcc agcagctcgc aaatggtttc cacaacatcg agattcttac gctcgttgtg
    26821 accaccaata ttataggttt caccgacttt cccggtggtc gccacgcaat acagcgcgcg
    26881 ggcgtgatct tccacataca gccagtcacg gatttgctgc ccgttgccat ataccggcag
    26941 cggtttaccc gccagcgcgt tgaggatcat cagcgggatc agtttttccg gaaagtggta
    27001 aggaccgtag ttattcgagc agttggtgat aagcgtcggc agaccgtagg tccgcagcca
    27061 ggcgcgcacc agatggtcgc tgctggcttt tgacgcggaa taagggctgc tcggcgcata
    27121 cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg tcaccatata cttcgtcggt
    27181 ggagatatga tgaaaacgga acgctgattt tttatcttcc gtcagcgtat tccagtaagc
    27241 ccgcgccgct tcaagcaatg tataagtccc gacaatgttg gtttcaataa acgctgccgg
    27301 gccgtcaata gaacggtcaa catggctttc ggctgccaga tgcatgacac agtctggctg
    27361 atgctcagtg aatacgcgtg ccagttctgc ccgatcgcag atatcaactt tctcaaaggc
    27421 aaagcgatcg ctttgcgcga ccggtgccag cgacatcagg tttccggcgt aggtcagctt
    27481 atcgaccact accaccgcgt cgctcgtttc gttgatgata taacgcacca gcgccgagcc
    27541 aataaacccg gcaccacctg ttatcagaat ttttctcatc agcgccagac tcctttggca
    27601 tcgacgacat actgctgatg gacattgtcg ccattgataa ctttaaactg actatgatcg
    27661 accagcatca ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc cagagtacaa
    27721 agcccggtca gttttttcgg caactggtgg atgttaggct caacaaccag agtttcgccg
    27781 ctatgccact gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg caggtcatca
    27841 atattcggtt taaacgccag accaaagcag gcgattttca gttcactggc gcgtttatcg
    27901 gtagccgcca ggcaatcagc caccgccgct ttcacctgat cgataaccca gaacggtttg
    27961 tgatcgttca cttcgcgcgc ggtacggata agccgcgcct gctgggggtt ctgtgccacg
    28021 ataaaccacg gatcaacagc aatgcagtga ccgcccacgc cagggccagg ctgaagaata
    28081 ttaacgcgag ggtgacgatt cgccaggcga atcagttccc agacgttaat cccctgatcg
    28141 gcacaaatca gcgacaattc attagcaaaa gcgatattca catcgcggaa gctgttttcg
    28201 gtgagcttac acatttccgc cgtccgcgag ttagtgacga cacactcacc ttcgaggaaa
    28261 attttgtaca gttcgctggc gcgggtcgaa caaaccggcg tcataccacc aatcacgcga
    28321 tcgtttttaa tcagctcgac cataacctgg cccggtaaca cgcgttccgg gcagtaagca
    28381 atgttgacgt ccgcctgctc gcccacctgc tgtgggaaag tgagatccgg acgcatctct
    28441 gctaaccatt ctgccatctt ctcggttgac cccaccggcg aggtggattc aaggatcacc
    28501 agcgcgcctt ttttcagcac tggcgcaatg gagcgagcag ccgattcaac gtaggtcata
    28561 tctggctcat gatcgccttt aaacggcgtg ggtacagcaa tcagccaggc atccgcttca
    28621 actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg cagtttttac tacgctcgcc
    28681 aaatcaggct cgacgatatg gatttcgcca cgattgatgg tatcaaccgc gtgttggttg
    28741 atatcgacac caatcacctg tttttgccgt gaggcaaacg ctgctgctgt aggcagcccg
    28801 atgtaaccca gtccgataac agaaatggtc gcaaaactca tagtgatatc cgattatttt
    28861 ttaacgcttc cagaatgcga gagcatgcct gaccatcacc atacgggtta tgggcgcggc
    28921 tcatagtttg atattcgttt tcgtctttta aaagacgcgt cacttcctcg acaattcgct
    28981 gcttatccgt gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga cgctcagtgg
    29041 tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc ttcttcctga atgccgcctg
    29101 agtcggtcaa aatcagccag gcgtggttca tcaaccagac aaacggtaaa tactcctggg
    29161 gatcgatcag aatgacattt ttcacatgcc ccagaatgcg attgaccggc tctctgacgt
    29221 tcgggttgag atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg gcaatgtctg
    29281 ccaacgcgtg gcagatttct tcaaaaccac gaccgaaact ctcacgcctg tgaccggtca
    29341 ccagaatcat ctttttattg gggtcgataa acgggtaatt tgccaccagt tctgaacgca
    29401 gcgtgtcgct gctcatcacc tgatcacgca cccataacag tgcatcaatg actgtattac
    29461 cagtaatgaa gattcggcta tccgcaacgt tttcacgcag caagttttgc cgggaagttt
    29521 cggttggaga gaagtgatac atcgccagat gcccggtcaa tgtacggtta gcctcttccg
    29581 gccacggcga atagagatcg cccgtgcgta aaccagcctc aacgtgacca acaggaatac
    29641 gctgataaaa cgccgccagg ctggttgcca gcgtcgttgt tgtgtcgccg tgaaccagca
    29701 cgacgtctgg tttgaactcg gcaagaatag gttttagccc ttccagaatc cgacaggtta
    29761 tctctgtcag gccctgtcct ggctgcatta tgttgagatc gtagtcaggt acaatggaaa
    29821 agagtttcag cacctgatcg agcatctccc gatgctgcgc agtgacgcaa actttagcct
    29881 caaaaaaagg agcttttgcc aacgcatgca ccaacggagc catcttgatg gcttccgggc
    29941 gcgtaccaaa tacagtcagt actttcacat cgattctctt cgaataagcg gcgagcgcct
    30001 ttgcgctcgc cgcagcagtg ttgctatttc gagcaacggc gggttaatgc gacaccagcc
    30061 ccgatcagcc ccccgacaat gccccacata atcatcagga aggcacgacg tgggctatcg
    30121 cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg cggatccagg
    30181 gttggaccaa cattcagggt gtttaacatg gcccgattct gatcatagtc gagatcaaag
    30241 gccggaccga cggcctgtaa attttccagt cgagcctgga gcattggacg cccaagcagg
    30301 aacatttctg aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg cgaaatatta
    30361 tgctgctcag caattttcag cgcctgctca atgctgttca tccggcggtc gtagatggct
    30421 ttcgccacct cttcctgacg cttcacctga gctttcatct ggatggtgcg tgccgcccat
    30481 gcgcctttca gctcatcatt cagatggctg gctgcacgct ggctggcaaa agcaacatac
    30541 tgacgtaaca ggttattagc gtcaggcgcg gtttcggcaa taagcttcac gctgtcattg
    30601 accgcgcggg taaagtctcc ggggataaac tggatgttgt taatcatttc atccagcaac
    30661 gccgcatcgg ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt ttgcagccag
    30721 aactctctgc gggtatccca cgaggccagt tgcataacaa actctttata ggcttcgtcc
    30781 atgaccgatg gttgatcggc agaagccatg tttgaacgga catccaggtt acgcaaaaat
    30841 tgctgctgcg agtaatatcc ccccagcata ttcaccgttg gacgatcggt aatcgccgtc
    30901 gagctccact cctgacgagc aaaaaaagta tacgccagcg cgattaacgc aaacgccagc
    30961 cccatgccaa taatccatag cttcccagcc cacaaggtac gaaacaaccc acgaatatcc
    31021 agttcatttt cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat cacatcctca
    31081 tttatttggt taaattgggg ctgccaccac gatttctacg cagtctgcgt tttacgcgct
    31141 taataaagcg agcaactttc caggcacgct taatgcaata tccatagagg aagaatgcta
    31201 gcaaaaagag caccagcatg acccactccg ggacaaaatg agaatattct gccagcacgc
    31261 caatggaagc gagcagtgct gcggcaaggg taatcagcac aaacgcctga cgggaagtaa
    31321 acccggcacg catgatcaaa tggtgaatat gctgacggtc aggagagaat gggctcatgc
    31381 ctttacgcag gcgacggtac ataatcgcca ccatatccat tagcggaatg gcgattatcc
    31441 acaaagcggt aaccgggctg atgggatggg ttttgccctg ggtcgtttcg agcaggatcc
    31501 agataacggt aaaaccaatc agcgtactgc ccgcatcacc cataaagact ttgtagcggc
    31561 gacccaggat accaaggtta agcatgatgt atggcaggat ggcggcgatc atcgcaaagc
    31621 accagattgc gaggctggtt tgcccgtcga accacaaaat cataccgatt gctgcaaacg
    31681 agacgcagga caacccgccc agcaagccat caatgccatc aaccatgttg aacgcattaa
    31741 tggccgccca gacggcaaat agcgtcagga agtaaccaaa cggtccgagc accatctccc
    31801 aggagccaaa gatataaccc aggctactga gataaagttt gccgaacacc atcataacaa
    31861 tgccaacagc ggcctgtatg gtggcacgga tttttacgct gatatcaaaa cggtcatcca
    31921 gcgcgccaat gaaaacaagc acaccggcac aagcgagata gagagatgca tgcggaatat
    31981 agtaatcgac aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc cccccaacga
    32041 gaggtatcaa tccctggtga cgtttgcgga agtttggttt atccactaaa ccgacttttt
    32101 ttgccacctt acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata ctgatgagat
    32161 cagtactcac tgtcagtaaa ttcacaatgc atgctctcag agaaaattat tagcagaagt
    32221 ataaccacga agacctttat tcagaaggga aagtccgttc cgaacccacc agtcctgtgc
    32281 aaaaattaac catttgatat gcaaattgca taacatgcca gcaaattcca ggaattttcc
    32341 taacatcgcc agcgcggcat taagattaca gatataaaag caaaacgcca cgtaaacacg
    32401 tggcgttttt ggcataagac aaatttatga gcgtttcatc atttcgaaga aatcgtcatt
    32461 ggtcttggtc atggccagtt tattaatgag gaattccatt gcatcgattt cgcccatcgg
    32521 gtgaatgatt ttgcgcagga tccacatttt ctgcagttct tcctgagtcg tgagcagctc
    32581 ttctttacgg gtaccggaac ggttgtagtc gatagccggg aagacgcgtt tttcagcgat
    32641 cttacgagag aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt aaataacttc
    32701 gtccatttta gaaccggtat cgataagcgc cgtcgcgata atggtcaggc tgccgccctc
    32761 ttccacgtta cgcgccgcac cgaagaagcg tttcggacga tgaagggcat tggcgtccac
    32821 accaccggtc aacactttac ctgacgccgg aacaacagtg ttgtaagcgc gcgccagacg
    32881 agtgatggag tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc gtttggcctt
    32941 ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa aggtagaagc
    33001 aacaacttca ccttttacca gacgctgcat ctcggttact tcttccggac gttcgtcgat
    33061 cagcagaacc atcagcacac aatccgggtg gttgtaagca atgctctgag caatgttctg
    33121 cagcagcata gttttaccgg ctttcggcgg tgccacaatc agaccacgct gaccacgacc
    33181 gataggtgat gccagatcca gtacgcgagc ggttaaatct tccgtagaac cgttaccacg
    33241 ttccatacgc agacgagagt ttgcgtgcag cggggttaag ttctcaaaga ggattttgtt
    33301 gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg caaaatagcg
    33361 ttcaccttct ttcggcgggc gaatcttacc agagatggta tcaccagtgc ggaggttgaa
    33421 acggcggatt tggctagggg aaacgtagat gtcatcagga ccggcaaggt aggagctgtc
    33481 tgcggaacgg aggaaaccaa atccatcctg caatatctcc agtacgccat caccaaagat
    33541 atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct gcttacgcat
    33601 acgagccagg ttttccagcc ccatattttc gccgagagtg atcagctcag aaaccggcgt
    33661 attctttaat tcggtaagat tcatagtggt gtgagttctt aaacttgggg taaatctcga
    33721 acttaatgtt gtgaatggta tggcaggatc atccatgcct gtttacggtc atcgactcat
    33781 gtcttttcgc tgcctggtca caggagagta cgcagaactg aaacgacaag acggattgag
    33841 tgacgagcca gaaatctgtt cacttcgcat ttaagataaa acgggaagcg ttggaaatta
    33901 caagattcaa acttaataag gtatgtttaa tacgaagtca acactaagtt agcatgactc
    33961 acgccgggcg tccagttttt agcgacgggg cacccgaaca tgaaattccc ttacgccagg
    34021 ttagcgtcga ggaactcttt caactgacct ttagacagtg cacccacttt ggttgccgcc
    34081 acttcaccgt ttttgaacag cagcagagtc gggataccac ggatgccata tttcggcgca
    34141 gtgccagggt tttgatcgat gttcagtttt gcaacggtca gtttgccctg atattcgtca
    34201 gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc tgcccagaaa
    34261 tcgacgagga tcgccccgtc cgctttgagt acatccgtgt caaaactgtc gtcagtcagg
    34321 tgaataattt tatcgctcat atataactcc acaggaataa gcctggcgtg ttggtttcgt
    34381 tgttggtgta acattaacca actaaaggtt gactttattt caccggatac gctttcgtaa
    34441 agcaatagta agctgatatt ctaccacact atgagcaaaa cacatttaac agaacagaag
    34501 ttttccgact tcgccctgca tccgaaggtt gtagaaatcc ttgaaaaaaa agggtttcat
    34561 aactgtacgc ccattcaggc actggccctt ccgctgacgc tggcgggtcg tgacgtagcc
    34621 gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac gtttcattat
    34681 cttctctctc atcctgcgat tgccgatcgc aaggtgaatc aaccacgtgc cttaattatg
    34741 gcaccgacgc gtgaacttgc cgtgcagatc catgccgacg cagaaccgct ggcggaagct
    34801 accggcctga agctgggtct ggcttacggt ggtgatggct acgacaaaca gctgaaagtg
    34861 ctggaaagcg gcgttgacat tctgattggc actacggggc gtttaattga ctacgccaag
    34921 cagaaccaca ttaacctcgg tgccattcag gtggtggtac tggacgaagc cgatcgcatg
    34981 tacgatctgg gctttattaa agatatccgc tggctgttcc gccgtatgcc gcctgcaaac
    35041 cagcgcctca acatgctgtt ctccgccacg ctttcgtacc gggtacgtga actggcgttc
    35101 gagcagatga acaatgccga atatattgaa gtggaaccgg aacagaaaac gggccaccgt
    35161 attaaagaag agcttttcta cccttctaac gaagaaaaaa tgcgtttgct gcaaacgctg
    35221 atcgaagaag agtggccaga cagagcgatt attttcgcca ataccaaaca ccgttgtgaa
    35281 gagatctggg gccacctggc ggcagatggt catcgtgtcg gtttgttgac aggcgatgtc
    35341 gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc gtggcgatct ggatattctg
    35401 gttgccaccg acgttgccgc gcgtggtttg catattccgg cagtgacgca cgtctttaac
    35461 tacgatttac ccgatgactg tgaagattac gttcaccgta ttggtcgtac aggtcgcgca
    35521 ggcgcaagcg gtcactctat cagcctggcg tgtgaagagt atgcattgaa tttgcctgct
    35581 attgagacct atattggtca ctcaattccg gtaagcaaat acaatccgga cgcattgatg
    35641 accgatctgc caaaaccgct gcgcctcacg cgcccacgta caggcaatgg tccgcgtcgt
    35701 actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac tcaaaatcat
    35761 tcaaactgta tcaagatggc aagagaatga atcccgatga gcatactgta gtaagtgatt
    35821 caggtgaaag aatgcagcca acacagagac agattgaagg atgaagagta tgggttccac
    35881 ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc tggttgtgcg
    35941 cgaggtggct ggaagcatcc agacgctgac gcgaataaaa cgcaaagtgc gtctggctgc
    36001 tggcctgaat agcgaaaatg ccctgtctaa tgaagcaatg gagcgcggtt ggcaatgtct
    36061 gcgcctgttt gctgaacgtc tgcaagatat ccctccctcg caaattcgcg ttgtcgctac
    36121 ggcgacatta cgtcttgccg tcaatgcggg tgattttatt gccaaagcgc aggaaatcct
    36181 cggttgtccg gtacaggtga tcagcggtga agaggaagca cgtctgattt atcagggcgt
    36241 tgctcacaca actggcggtg ccgatcagcg cctggtggtg gatataggcg gtgccagtac
    36301 tgaactggta accggcacgg gtgcacaaac cacctcgttg ttcagcctgt cgatgggctg
    36361 cgtcacctgg ctggaacgct attttgccga tcgtaatctg gggcaggaaa attttgatgc
    36421 tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc gatgaattac ggtatcacgg
    36481 ctggaaagtg tgcgttggtg cttccggcac cgtgcaggcg ttacaggaaa tcatgatggc
    36541 acaggggatg gatgaacgca ttaccctgga aaagttgcag caattgaaac agcgagccat
    36601 tcattgcggt cggctggagg aactggagat tgacgggctg acgctggaac gtgcgttagt
    36661 gttcccgagt ggtctggcga tcctgatcgc catttttacc gaactgaata ttcagtgtat
    36721 gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc atctggccgt
    36781 cgagcaggat attcgcagcc gtacgctgcg taatattcag cgccgcttta tgatcgatat
    36841 tgatcaggcg cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg tggaaaagga
    36901 atggcatctt gaagcaataa gccgcgattt gctcatcagc gcctgtcagc ttcatgaaat
    36961 cggcctgagc gttgacttta aacaagcgcc gcaacacgct gcttatctgg tgcgtaatct
    37021 ggatcttccc ggttttaccc ccgcacagaa aaaattgctg gcgacgctac tgctcaacca
    37081 gactaatccg gtcgatctct catcgctgca tcagcaaaat gccgtaccgc cgcgcgtcgc
    37141 agaacaactc tgccgtttac tccgcctggc catcattttt gccagccgtc gccgtgacga
    37201 tctcgtgcca gagatgacat tacaggctaa ccatgaactg ttgaccttga cgcttccgca
    37261 aggttggcta acccaacatc cgctgggtaa agagattatt gctcaggaaa gccagtggca
    37321 gagctatgtc cactggccgc tggaagtgca ttaatgagta agtgccggat gcgatgctga
    37381 cgcatctttt ccggccttga ttatttccct cgttttgccg ccatcatcgc tttcagattc
    37441 gccagatggc tttgcccttt ctgcatccgt tcttcggcgc tgaccacttt gcgctcctgt
    37501 tcccaaatca gatcatcctg cggcagctcc agcaaaaagc ggctcggctc cgggcgcacc
    37561 agctcgccgt actgacggcg ttctttacac agcgtaaagg tcaattcctt ctgggcgcgg
    37621 gtaatgccga cataggccag ccgccgctcc tcatcgatat tatcttcatc gatgctgctc
    37681 tggtgcggca aaaacccttc ttccataccg accatgtaga cataaggaaa ctccagccct
    37741 tttgacgcgt gcagagtcat cagttgcacc tgatccagct cttcttcact ctcaccacgc
    37801 tccatcatgt cgcgcaaagt aaagcgcgtc accacctggg tgagcgtcat cggctcatcc
    37861 agttcgctgc cttccagcat ctccgtcatc cagctaaaca gttggttgac gttcttcatg
    37921 cgcatttcgg cggctttcgg gctgggcgat gtttcgtaca gccaggattc ataatccatg
    37981 ccgtggatca gatcgcgcac cgcggcaatc ggctcccgct ccgccagacg ctggatttct
    38041 gccaaccagt gagtaaagcg ggtcaatgct tcataaccac gtccgctaag cgtctgactc
    38101 aggcccatat caaagctggc ggtaaacatg cttttattgc gcgtcatcgc ccactcaccc
    38161 agctttttca gcgtcgccgg gccaatctct ctcttcggcg tgttaacgat acgcagaaat
    38221 gcgctgtcat cgtccgggtt agtcagcacg cgcagataag ccagcaagtc cttgatttca
    38281 ggacgagaga aaaacgacgt accaccagaa attttgtacg ggatgcggtt ttgcatcagg
    38341 aatttttcaa acactcgcga ctgatggtta ccgcgataaa gaatagcgta atatttgtac
    38401 tgcgttttat tgacgaagtg atgcgcaatc agttcaccag tgacgcgctc ggcttcatgt
    38461 tcttcgttat tcgcgcttaa aactttaagc tctgtgccgt aacccagttc ggagaacaga
    38521 cgcttctcaa agacgtgcgg gttattggcg atcaggatgt tcgccgcttt caaaatacgt
    38581 ccggaagagc ggtagttctg ctcaagctta atcaccttca gcgccggaaa atcctgactc
    38641 agcagcacca ggttttgtgg acgtgcgccg cgccaggagt agatcgactg gtcgtcatcg
    38701 cccaccacgg taaagcgcgc gcggctgccc accagcagtt tcaccagttc atactggctg
    38761 gtgttggtgt cctgatactc atccaccagc agatagcgaa ttttgttctg ccagcgctca
    38821 cggacttctt cattgcgttg cagcagcaac gtcggcaata aaatcagatc atcgaagtcg
    38881 agaacattgc acgctttcag gtgtgcatca tacagcccat aacaatgggc aaaaatacgg
    38941 tcccgctcgc caatcgcact ggctgccgcc tgggacggtg ttttgagatc gttcttccag
    39001 ttagagatgg tcgaaatcaa ctgttgcagg agaactttgt catcttcaat cagcccttcg
    39061 gtcaactctt tgagcaacgc cagctgatcg gtatcgtcaa acagcgaaaa gttcgccttc
    39121 atccccagcg ccgcatactc acgcttgatg atatccagcc ccagcgtgtg gaaggttgag
    39181 atcatcagcc cacgcgcctc tttgcgaccc agcgtctgtc cgacacgctc tttcatctcg
    39241 cgcgccgcct tattagtaaa ggtcaccgcc gcgatatgtc gcgcctgata accgcaaccg
    39301 cggatcagat gggcgatttt attggtgatc acacgagttt taccggaacc cgcgcccgcc
    39361 agcaccaggc aggggccggt aacgaattcg acagcttgtt gttggccggg gtttagacgc
    39421 ataggtgtat tgctcaatct tcgaacgggg ggaggattgt agcagaatcg catttgaatg
    39481 tgaccgcgtg taagccgcta accggttggc tgcggatgtt taaatcagag agggaagacc
    39541 gaaaccggag ttcatcgtga atccattaga cttagaaaat atcgggtcgt aacgttgtgt
    39601 tatta
//
LOCUS       DAJTOR010000015        37818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTOR010000015 DAJTOR010000000
VERSION     DAJTOR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.015"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..392
                     /locus_tag="OO249_000724"
     CDS             <1..392
                     /locus_tag="OO249_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5635122.1"
                     /translation="HYPLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEI
                     DEKFMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKPEHPLPSI
                     KLYISYNKSSLNNLVFSRFIDRLNESF"
     gene            539..2056
                     /locus_tag="OO249_000725"
     CDS             539..2056
                     /locus_tag="OO249_000725"
                     /inference="COORDINATES: protein motif:HMM:NF014273.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5635123.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEDDRIKAINEIRMAIHQ
                     VSPFREEPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDK
                     NAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQIT
                     AMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2057..3206)
                     /gene="ybdL"
                     /locus_tag="OO249_000726"
                     /pseudo
     CDS             complement(2057..3206)
                     /gene="ybdL"
                     /locus_tag="OO249_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            3315..4403
                     /gene="hcxA"
                     /locus_tag="OO249_000727"
     CDS             3315..4403
                     /gene="hcxA"
                     /locus_tag="OO249_000727"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5635124.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4413..4610)
                     /locus_tag="OO249_000728"
     CDS             complement(4413..4610)
                     /locus_tag="OO249_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5635125.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4792..6897)
                     /gene="cstA"
                     /locus_tag="OO249_000729"
     CDS             complement(4792..6897)
                     /gene="cstA"
                     /locus_tag="OO249_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5635126.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7078..7491)
                     /gene="entH"
                     /locus_tag="OO249_000730"
     CDS             complement(7078..7491)
                     /gene="entH"
                     /locus_tag="OO249_000730"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5635127.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7494..8240)
                     /gene="entA"
                     /locus_tag="OO249_000731"
     CDS             complement(7494..8240)
                     /gene="entA"
                     /locus_tag="OO249_000731"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5635128.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8240..9097)
                     /gene="entB"
                     /locus_tag="OO249_000732"
     CDS             complement(8240..9097)
                     /gene="entB"
                     /locus_tag="OO249_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5635129.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9111..10721)
                     /gene="entE"
                     /locus_tag="OO249_000733"
     CDS             complement(9111..10721)
                     /gene="entE"
                     /locus_tag="OO249_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5635130.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATASPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQWLASRASA"
     gene            complement(10731..11906)
                     /gene="entC"
                     /locus_tag="OO249_000734"
     CDS             complement(10731..11906)
                     /gene="entC"
                     /locus_tag="OO249_000734"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR5635131.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVLASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12281..13237
                     /gene="fepB"
                     /locus_tag="OO249_000735"
     CDS             12281..13237
                     /gene="fepB"
                     /locus_tag="OO249_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5635132.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLSGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13241..14491)
                     /gene="entS"
                     /locus_tag="OO249_000736"
     CDS             complement(13241..14491)
                     /gene="entS"
                     /locus_tag="OO249_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5635133.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWLVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14602..15606
                     /gene="fepD"
                     /locus_tag="OO249_000737"
     CDS             14602..15606
                     /gene="fepD"
                     /locus_tag="OO249_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5635134.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15603..16595
                     /gene="fepG"
                     /locus_tag="OO249_000738"
     CDS             15603..16595
                     /gene="fepG"
                     /locus_tag="OO249_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5635135.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16592..17407
                     /gene="fepC"
                     /locus_tag="OO249_000739"
     CDS             16592..17407
                     /gene="fepC"
                     /locus_tag="OO249_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5635136.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIKHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(17404..18537)
                     /gene="fepE"
                     /locus_tag="OO249_000740"
     CDS             complement(17404..18537)
                     /gene="fepE"
                     /locus_tag="OO249_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5635137.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18752..22633)
                     /gene="entF"
                     /locus_tag="OO249_000741"
     CDS             complement(18752..22633)
                     /gene="entF"
                     /locus_tag="OO249_000741"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5635138.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22630..22848)
                     /gene="ybdZ"
                     /locus_tag="OO249_000742"
     CDS             complement(22630..22848)
                     /gene="ybdZ"
                     /locus_tag="OO249_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5635139.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(22851..24053)
                     /gene="fes"
                     /locus_tag="OO249_000743"
     CDS             complement(22851..24053)
                     /gene="fes"
                     /locus_tag="OO249_000743"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5635140.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24296..26536
                     /gene="fepA"
                     /locus_tag="OO249_000744"
     CDS             24296..26536
                     /gene="fepA"
                     /locus_tag="OO249_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5635141.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26711..27331
                     /gene="entD"
                     /locus_tag="OO249_000745"
     CDS             26711..27331
                     /gene="entD"
                     /locus_tag="OO249_000745"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5635142.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27453..27605)
                     /gene="hokE"
                     /locus_tag="OO249_000746"
     CDS             complement(27453..27605)
                     /gene="hokE"
                     /locus_tag="OO249_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5635143.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28059..29177
                     /locus_tag="OO249_000747"
     CDS             28059..29177
                     /locus_tag="OO249_000747"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5635144.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQVTVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29243..29491
                     /locus_tag="OO249_000748"
     CDS             29243..29491
                     /locus_tag="OO249_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5635145.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPTPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29556..29924
                     /locus_tag="OO249_000749"
     CDS             29556..29924
                     /locus_tag="OO249_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5635146.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30018..30671
                     /gene="nfsB"
                     /locus_tag="OO249_000750"
     CDS             30018..30671
                     /gene="nfsB"
                     /locus_tag="OO249_000750"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5635147.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            30779..32026
                     /gene="ybdG"
                     /locus_tag="OO249_000751"
     CDS             30779..32026
                     /gene="ybdG"
                     /locus_tag="OO249_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5635148.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(32094..33470)
                     /gene="pheP"
                     /locus_tag="OO249_000752"
     CDS             complement(32094..33470)
                     /gene="pheP"
                     /locus_tag="OO249_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5635149.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYICSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33572..36699)
                     /gene="cusA"
                     /locus_tag="OO249_000753"
                     /pseudo
     CDS             complement(33572..36699)
                     /gene="cusA"
                     /locus_tag="OO249_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(36711..>37818)
                     /gene="cusB"
                     /locus_tag="OO249_000754"
     CDS             complement(36711..>37818)
                     /gene="cusB"
                     /locus_tag="OO249_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR5635150.1"
                     /translation="RKILFWYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVR
                     IDPTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVG
                     DKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLI
                     ATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWL
                     VKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWL
                     QLNTASEPMLLIPSQGLIDTGSEQRVITIDADGRFVPKRVAVFQASQGVTALRSGLAE
                     GEKVVSSGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         9074 a        10550 c         9629 g         8565 t
ORIGIN      
        1 gtcattaccc tctaatttgt agtaataagc atccgcgaat tactgacaat agcacttatg
       61 aacaaattat ggcagaagag ttcacccaat taatatccaa atcagcagga gttgatgata
      121 ttcaaatgga aatagacgag aagtttatga atcgcaaaat ttcatttcgt ggttcctctt
      181 tgctgacaat aattaatagc attgcagtta cagatttact gggaatagtc ccctatgaac
      241 tttataactc ccaccgtgac tttcttaatt taaaagagat aaaaccagaa catccgttac
      301 cttcaataaa gctttacatt tcctataata aatcttcttt aaataatttg gtgttctccc
      361 gatttattga ccgtctaaat gagagttttt gatatataac tacagacagc tactataact
      421 ttcatctatt tattcacagc gcgaatgaaa aataaaacac tctgctttac agtgtttact
      481 ttataattaa cagtcttatc tcactgttca ccctgtaacg ttcagagagc gtcttcagat
      541 gtctatttat aaaattcctc ttccgctcaa tattctggaa gctgcacgag agcgtattac
      601 ctggacactc aacaccctac cccgcgtttg tgtctcattt tctggcggca aagactctgg
      661 tttaatgctg catctgacag ccgaacttgc ccgacaaatg ggcaaaaaaa tctgcgtttt
      721 gtttatcgac tgggaggcgc aattctcttg cactattaac tatgttcagt ccctgcgcga
      781 gttgtacacc gatgtcatcg aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa
      841 ttccctttca caataccaac ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg
      901 tcagcccccg caagatgcga taaccgatcc tgactttttc tgcttttacc agccaggcat
      961 gaccttcgaa caatttgtac gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc
     1021 gatgatgatc ggcatccgtg cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt
     1081 aaataaacaa cgttttgccg acgataaacc atggactacg gccgcaccag gcggtcatag
     1141 ctggtacatt taccccattt acgactggaa agtggctgat atctggacct ggtatgcaaa
     1201 tcatcaaagt ctctgcaacc cactgtataa cctaatgtat caggcaggcg ttcctctgcg
     1261 tcatatgcga atttgcgaac cttttggccc ggagcaacga caaggattat ggctctatca
     1321 cgttatcgaa ccggatcgct gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg
     1381 cggcatttac gccggacatg acaatcattt ctatgggcat cggaaaatcc tcaagccaga
     1441 acatttagac tggcaagaat atgcattatt gctgctcaat agcatgccgg aagatgatcg
     1501 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
     1561 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
     1621 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
     1681 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
     1741 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
     1801 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
     1861 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
     1921 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
     1981 gcgcctgaag caaattaacg gcctgcaaga gttgtttgcc gaccgtcaat attcccgcgc
     2041 ctggacggtt aaatagctaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
     2101 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
     2161 acagcggaat cgccgccacg ccgtgctccc gcgtcagcca ctggcaaaac tcaacatcat
     2221 ccagggtaga aacctcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
     2281 tctccagccg gctttcattt aaagcattca ccagaatatc gcgcttctgg cgataaaagt
     2341 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
     2401 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
     2461 gcgctggcgc aacacaataa cccactttcc agccggtcat atgatagatc ttgccaaatg
     2521 aagaaaccgc cactgcccgc tcacgcagct gcggatgcgc cagcacactg gcatggccct
     2581 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
     2641 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggggttat
     2701 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
     2761 agtcaaggtg gttgcagtgc catacgcttc actattcccc cagaaagcgc gatggcgggg
     2821 gcgtaactgt catagctggg atcaaaacaa atcacttcat cgccattgcg caccagtgcg
     2881 gtaatcgccg cgtataacgc ttccgtcgcc cctgccgtta cggtgatatc gctatcggca
     2941 tctggttgat agccatacaa acgttccgtt ttctgagcaa tcgcctcgcg caaggcctgc
     3001 acgccggtca tgggcgcgta ttggtttgcc ccctgggcaa cgtggtacgc cagccgctcc
     3061 tgtaaatagc gcggaccatc aaaatcagga aagccttgcg acaggttaat cgcctggtgt
     3121 tgctgcgcca gcgcgctcat ctgggtgaaa atagtggtgc caagttgtgg aagtttgctt
     3181 tgtggaatca gagggttatt tgtcattttt attgtacctg acgaagttgc gtgttgaagg
     3241 cactataaca cgacgctagt atttggcaat caagacgttt agatgtctaa ataaaacaat
     3301 aaggacaaca caacatgcct cacaatccta tccgcgtggt cgtcggcccg gctaactact
     3361 tttcacatcc aagaagtttc aatcacctga acgatttttt cactgatgaa caactttctc
     3421 gcgcggtgtg gatctacggc aaacgcgcca ttgctgcggc gcaaaccaaa cttccgccag
     3481 cgtttggact tccaggggca aagcatattt tgtttcgtgg tcattgcagc gaaagcgatg
     3541 tacagcaact ggcggctgag tccggtgacg atcgcagcgt ggtgattggc gtcggcggcg
     3601 gcgcactgct cgacaccgcg aaagccctcg cccgccgtct cggtctgcca tttgttgccg
     3661 ttccgacgat cgccgctacc tgcgccgcct ggacaccgct ctccgtctgg tataacgatg
     3721 ccggacaggc gctgcattat gagattttcg acgacgccaa ttttatggtg ctggtggaac
     3781 cggagattat cctcaatgca ccgcaacaat atctgctggc ggggatcggt gacacgctgg
     3841 cgaaatggta tgaagcggtg gtgctggctc cgcaaccaga aacgttgccg ctaaccgtgc
     3901 ggctggggat caataatgcg caggccattc gcgacgtctt gttaaacagt agcgaacagg
     3961 cacttgccga tcagcaaaat cagcagttaa cgcaatcatt ttgcgatgtg gtggatgcta
     4021 ttattgctgg tggtgggatg gtcggtggtc tgggcgatcg ttttacgcgt gtggcggcgg
     4081 ctcatgccgt gcataacggt ctgactgtcc tgccgcaaac cgagaagttt cttcacggca
     4141 ccaaagtcgc ttacggaatt ctggtgcaaa gcgccttgct gggtcaggat gatgtgctgg
     4201 cgcaattaac cggggcgtat cagcgttttc atctgccgac tacgctggcg gagctggaag
     4261 tggatatcaa taatcaggcg gagatcgaca aagtgattgc ccacaccttg cgcccggtgg
     4321 agtccattca ttacctgcca gtcacgctga caccagatac gttgcgtgca gcgttcgaaa
     4381 aagtggaatc gtttaaagcc tgacgtgcaa atttagcagc agcgcgcgcc gccttttcca
     4441 ccgtagcgcg cgtcctgccg ctcacggaaa aactcctcgt aggtcatcgg cgtttgatcg
     4501 ggatggttaa cccgcatatg ttcgacatag ttgtcgtaat caggcatacc aatcatcagc
     4561 ttcgccgcct gacctaaata ttttccggct tttgccagtg aatcaaacat cttggcctcc
     4621 gctaaccatg ttgctgaaag agtgccggat gcggcgtgaa cgctttatcc ggcctacaaa
     4681 tgctatcaac gcactcggtc agtcccctcg ccccatcggg gagagggtta gggtgagggg
     4741 gaaccctcgg cactatgcca acattcgcca acatcccccc tcactctgac tttagtgcgc
     4801 gcctttcgcc tgcgctacga tctcctcgac attttccggc atcggctcat acggcgtttc
     4861 tttcgccgtt ggcttcggat ctttcaatgc cgcaagtgcc gtcttaatcg agaacagtgc
     4921 cagaaccacc acgaccacca taaagaagat ggttaacccg gcatccagac ggttgttgaa
     4981 caccagttgc gccagctgtg actcagtata ctgcgacgga atattgccgc tgtcgatcat
     5041 tgcctggaac ttgttagcaa tggccaggaa gccgactttc gcatccgggc taaacgcttt
     5101 ctgccagcca gcggtcaggg tacaaatcag cagccaggcc gttggtacca gcgccaccca
     5161 ggcgtaacgt tgacgtttca tcttgaacaa caccacggca cagagcatca gcgccatccc
     5221 tgccagcatc tggttggcaa taccaaacag cggccacaga gtgttaatac cgcccagcgg
     5281 atcgaccacg ccctgatgga ggaagtagcc ccacgccagt acgcacagcg ccgttgccag
     5341 caggttagca ggcagtgaat cggtccgctt caggccagga gacaccacgc ccagcagatc
     5401 ctgcaacata aagcgcgcag cacgcgtacc cgcatctacc gccgtcagaa taaacagagc
     5461 ttcaaataga atggcgaagt gataccagaa cgccacatcc atcataccac ccagcgcacc
     5521 gtgcaggatg taagccatcc ccaccgccaa cgtcggcgca ccgcccgcac gggaaatgat
     5581 cgactgttca cccacttcgc tggcaatctg gttgagcgta tctggtgtaa tgctaaagcc
     5641 ccagctactc accacctgcg cggcagaagc gaccacatcc gccgtccctg ctggagccag
     5701 caccgccatt gggctgttca tggcaaagta aacgcccgga tcgatgatac aggcggaaac
     5761 cagcgccata atcgccacga aggattccat taacattcca ccgtagccga taaaacaagc
     5821 ctgcccttcg ttcgccagca tcttcggcgt ggtgccggaa gagatcagcg catggaagcc
     5881 agacaccgcg ccacaggcga tggtgataaa caggaacggg aacaggttac cggtccatac
     5941 cgggccagtg ccatcgacaa atttggtcag cgcaggcatg gtcagcgtcg ggcgcataat
     6001 caaaatgcct accgccagac cgacgatcgt cccgattttc aggaaggtag agaggtagtc
     6061 acgcggggcc agcagcaacc acaccggcag caccgccgcc acaaaaccgt aacccaccag
     6121 catccaggtc agctgcacgc cggtaaagtc aaagtacggt gcccaggtcg gactttctgc
     6181 cacccagccg ccagagataa tggcgaaaat caggaatacc agaccgatga ccgacacttc
     6241 accaatacgc cccggacgca gatagcgcag gtagatcccc ataaacagcg ccagcggaat
     6301 ggtgaacgca acggtatacg ttccccacgg gctatgagtc agggctttca ccacgatcat
     6361 cgccagtact gcaaggataa tgaccatgat cataaagcag gccaccagcg ctatcacccc
     6421 ggcggttggc cccatctctt ctttgaccag ctcacccagc gatcgaccgt cacggcgcgt
     6481 agaaacaaac agcaccatga aatcctgcac cgcaccggcg agaaccaccc cggcgagtag
     6541 ccagatcatc cccggcaggt agcccatttg cgctgccagc accggcccaa ccaacggacc
     6601 tgctccggcg atggccgcaa aatggtgacc gaacagcact ttcttgtccg tcggcacata
     6661 gtccagcccg tcgttatggc gcaccgctgg cgtcatacgc ttcgggtcaa ccgccagcac
     6721 atttttggcg atatacaacc cataaaaacg gtaagcgatc agatagatac agaccgacgc
     6781 caccacaatc cacagcgcgt tgatctgttc cccacgattt aaagcaatgt atcccagagc
     6841 aaatgctccc attacagaga gcactgtcca gacgaggtat ttccctgatt tgttcatagt
     6901 tgttatccgt gtgcgtgtcc atagagatgt tacattttgt ttctataaca tctttgtacg
     6961 atttaacaac acggtaacta tgtaaatcgg agttgaaacc aatatttaac ttaacaatgt
     7021 taactcgatc actccgttaa ccgagttttc ctgcaatctc atttaattct gtcgggctca
     7081 tcccaaaact gccgtaccca gccgacaagt gcagcaacgc cgcccctgtt catcgaaaac
     7141 gacgatttcc cagctctgat tttgccgccc aagatgcagc ggctggcaga cgccgcgtac
     7201 cttgccttca gacaccgggc gatgatgggt ggcgttaagt tccgtgccca ccacacactg
     7261 cccgtcacgg gtcatcataa atccggccat cgatcccagc gtttcagcca gcgccgccga
     7321 cgcgccgcca tgcagcaggc caaacggctg atgagtacgg gtatcaaccg gcatttcggc
     7381 ttccagcaca tcatctccca gacgggtata cacaattccc agatgcgcca ccattgtgtt
     7441 atcgctggtg gcgttcagtt cgtcgagcgt taaatggcgt ttccagatca tgcttatgcc
     7501 cccagcgttg agccgccatc gaccacaata tcctgtaggg tgatatggct ggcgaggtca
     7561 gaggcgagga acaaaatcgt gttggcgatc tcttgtggac gggcgatttt ccccagcgga
     7621 atgccgagtt taaactgctc gccaaagccg cgaatacgct gttcttcggc gtcatcgctc
     7681 acccacagcg tgcgttgcat atcggtgtcg gtggagccag gcgaaaccac attacagcgc
     7741 acgccgctac ccgccagttc cagcccgacg ctcaacgcca ggcttttcag cgccgctttc
     7801 gatgcgccat aagcactcat accaatacgc ggcgtgtgtg cggcgtcgga cgccacagtg
     7861 acaatcgccc cgccccgctg acggcgaaac tggttcatgg tttgctggaa caggttaaac
     7921 gcaccgccga cgttaaccgc aaaagtctgc tgccagtcct ctttactgag ctgatcggtc
     7981 gcgcccatgc gcaaaattcc cgccgcattg atcagcacgt ccagtcgctc cgtttcagct
     8041 aacagtcgct gacatacctg cgccacctgc ccggcgtcgg caacatccat tacttcggtc
     8101 gcaaagggat attgctcctg agtgaacgct tgatcaaaac ctgtaacttt cgctcccgcc
     8161 tcaacaaacg ccagcgccgt ggcgtagccg atacctttac ctgcgccggt tacccagaca
     8221 tttttaccgc tgaaatccat tatttaacct cgcgggagag tagcttccac caggcgtcga
     8281 tggtcgggtt tttcgccagc atgacaaagt cgatatcacc atgcactttg cgccagcgcg
     8341 ccgccagcgc catcatgcgc accgaatcca gaccgtagtc gatcaggttg tcgtcatcga
     8401 acggttcatc ggactcgtcc agcaacggca ggataacctc acgcagcgcc gctttgctgg
     8461 cggggacagg tgctggcagt aattcttcag tcatcaccac ccggccagac cgcccggcca
     8521 catatttcag cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca tccgccacca
     8581 taaacggttt aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag ccaatgtggg
     8641 catatacccc ggtaataatc agctggttac gtccactctc tttgagcatt tgctccagcg
     8701 gagaacgatg aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg gcatctggcg
     8761 tcaggcgatc caccaccttt tgctgttccg gcgaacgggt caggcccggc ccccacatat
     8821 cattcaacag cgcccgatct tcatcgctct gctctttcgg ctgggcggtg taataaactg
     8881 ggatattgtg ctgtttgcag tagtcgcgca gcgcagcaat attcgcgatc acctgctcca
     8941 tcatcgggca gttctcgccc cagaagctga caaaatagtc ctgcatatca tggattaaca
     9001 acgcggcacg ttgcagttca aaggcccagt caactttatt ctgcggaata tcgtgagact
     9061 ccggcagtgc gtaagcctgt aattttggaa tagccatcgt gttctctcct tcaggctgat
     9121 gcgcgtgacg ccagccactg acttaattgt tttttatcga ctttcccgac cgccgtcagc
     9181 ggaagtgaat ctacacactc cacgcgatcc ggtaatttaa attcggcaat accctgttca
     9241 cgcaggaaac gacgcacctg caccgcgcgc agcggctctt ttaccaccag ataagcacag
     9301 cttttttcgc ccatcagctc atcttccatg ctcaccagtg cggcatagat caccgccgga
     9361 tggcgcagca gcaggttttc gatctcttcg gcagcgatct tctcgccgcc acggttgatc
     9421 tgatctttct cgcgcccctg cacggtgatg taaccctctg gatcaataga gatcagatcg
     9481 ccggaacagt aaaaaccgtt ggcatcaaag gcgctggcat tgtgctgcgg acttttgtaa
     9541 taaccgcgga aggtgtacgg cccgcgcgtc atcaggcgtc cgacttcccc ttgcggcagt
     9601 ggatttcctt cggcatcggc aacccacact tcatcgtccg gacacattgg gtaaccctgg
     9661 gtatggataa ttttctccgc gctatcatca agacgggtgt agttcaccag cccttccgcc
     9721 atgccaaaca cctgctgcaa ctggcagcca atctcagcgg gaatacgcgc cgcaagggtg
     9781 gcagaaagac gtgcgccgcc gacctgtaac agtttcagcg aggcaagctg cgcccggctt
     9841 tcgccttcag ccagcgcctg caaccacagg ctgactgccg gcggcaccag cgcggtgacg
     9901 ttcacctgat gtttttcaat caatgggaag caaagcgtag cgctgggatc ggccgccaga
     9961 acaaccgttc ctccggcaag aaagacgccc agcgatcctg gcgaactcat ggcgtagtta
    10021 tgagccgccg ggatcgcgca caggtagcgc gtctgttgtg tgaactgaca aatctcgacg
    10081 ctacgacgca cgctgtagta gtagtcgtta tgagtgcgcg ggatcagctt cggtgtaccg
    10141 gtggtgccgc cggaaagctg gaaataggcc acttcatcag caggtgatgc cgtggcagta
    10201 aaaccgtctg ccggatggtt aatcgcatcc tgcaagttat gctcaccgct gtcgttgtgc
    10261 agttgcacca cgcgaatgga ggaatgttct gtgacgaaag tattgaggaa atcatccccg
    10321 ctaaacagcg catgttggcg atcggcaatc agcaatgcgg gttcaatctg gctggcatag
    10381 gcgttcagtt cactacgctg atggctgaac aacgccagca ccggcgcaac gcccagtttc
    10441 agcagcgcga aaaaggtaat atacaattca gcgacgttac ccagttgtac cagcgcggtt
    10501 tcaccaggtt taatgccctg acggcgtaaa gtacacgcga ggttatccgc cgcctgattc
    10561 agctcccggt aactcaactg tagctcgccg tcgataaccg cgatgctgtc actcgcagcg
    10621 tggcgagtca gaatgtcggt cagcggcaaa tcctgccagt agcctttttc ccgatagcga
    10681 cgggcaaact cttccggcca gcgggtgaat ggaatgctca tcctcgctcc ttaatgcaat
    10741 ccaaaaacat tcaacatggt agaaagtttg acgcctgttt cgcgccactc acccaacggt
    10801 gacgacgcaa gcacaatccc cgctccggca aacagacgca cctgattttc ccgcagcttc
    10861 gcgcagcgga tggtcaccac ccattcgccg ttaccttcgc tgtcacacca acccacaatg
    10921 ccgccaaaca gttcgcggtc gaacggctcc agttcagcaa taacctgagt cgcggcctga
    10981 tgcgggaaac cgctcagcgc gggggtcgga tgcagcagac aggccagagt cagtgcgttt
    11041 tcttgcgaat tcgctttacc ttcaaaggga gttgcgagat gccacagcgt cggcgtggta
    11101 atcaattgtg gagaggaggg aacgtgtaac tcactactgc gttcgcgcag tacctctttc
    11161 atcgcctgag tcaccagttc atgttcatgg cgatcttttt ctgacgccag cagacgatta
    11221 cccgcttcgc gatcgagcac ttcatccggc tgacgacgcg cggaaccggc taacggaatg
    11281 gagctaaaac gctcgccgtc tttacgtagc agcagttccg ggctggcccc cagcaggacg
    11341 ccaccatcag ccagcgggac atggaagttg taactaaccg ggttttgcgc aatcaaccgt
    11401 tccagcaata cgccactatc aatggcggcg tcagtggtga tatcaatcaa ccgtgacaac
    11461 accactttgt cgacctgcgg cgtggcggta agtgcggcag cgcgggcaac catctgttca
    11521 aacgtggttt gttccggaat tgcctggcgt tccaccacat tcagcgactg gctgcgggtg
    11581 aaacggcggg cggaagcttg tttttcctga cgggagaacg actgccagga ttcaggaata
    11641 tacagcgacg aaggctgacg tggatcgaag ggaatcgccc cgaccatcac cggatttttg
    11701 atgccctgca ctttggcatc ggcaaacagc gcggcgagtt tttgctggaa gggactgtcg
    11761 ggcgaatccc cgttcacagc cggttcatcg aagcgggcga aacatcctga cgtcgtaaaa
    11821 ctgcggtacg gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat ggtctgctgt
    11881 acttcctcag ccagtgacgt atccatatca tcctccacaa aatgataaag gctttaataa
    11941 tgattatcat ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta tgtcaactct
    12001 tgaggtaacg caatctgcaa ctcggggttg catctggttg tggttacaat gaaaatgaga
    12061 agcattattg atggattcgc ataagcgcaa tgtgatgtcc tgcgccgttc ttccccctct
    12121 cccttccagg gagagggctg gggtgagggt caatattcgc accagtgctg gctgctccca
    12181 tcaccctaac cctctcccca aaggggcgag ggtacggata gtgcgctttg tcgaatttgt
    12241 cattacgccc ttaaccttat taataacagg aagctgattt gtgagactcg ccccgctcta
    12301 ccgcaacgcc cttctattaa caggactttt gctttccgga atagccgcag ttcaggccgc
    12361 cgactggccg cgtcagatta ctgacagccg gggcactcat accctggaaa gccagccgca
    12421 gcgtattgtt tccaccagcg tcaccctgac cggctcactg ctggcgattg atgctccggt
    12481 gattgccagc ggcgcgacca cgccaaataa ccgcgtcgcg gatgaccagg gctttttacg
    12541 ccagtggagc aaggtggcga aagaacgcaa actacaacgg ctctatatcg gcgaaccgag
    12601 cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga
    12661 ttcggcgctg gcgctgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta
    12721 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    12781 acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat
    12841 caaattaccg ccgcagccgg tcactgctat tgtctatacc gccgctgcac acagtgccaa
    12901 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    12961 gaaattgccc acagggttaa acgccagcca aagccagggc aaacgccatg acatcattca
    13021 gcttagtgga gaaaatctgg ctgcagggtt aaatggcgag tcactattcc tgttcgccgg
    13081 tgatcagaaa gacgccgatg cgatttatgc caatccactg ctcgcgcacc tgcccgcagt
    13141 acaaaacaag caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc
    13201 catgcaagtg ctggaacgac ttaaggcgct gttttaagca ttaactgtcg gacgctgtca
    13261 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    13321 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgctc
    13381 ccagaccgcc taacagcgcc gcgcctatgg catcgcccgt cacgttttgt gccgtccaca
    13441 aaccgttgat tcgccctaac attgcttccg gcgtttgcgt ttgcagcatt gtatattgca
    13501 gcaacgaact gaccgcactc agccagccga acagcgccag acaaaccacg cctaaaatcc
    13561 acatcggcat caggccaaat aaaccaatgg cgaggaacga tcccaacgtg gagagcaaca
    13621 tcaacaatcc tggtcgcgcg ctatgcgcca gtttcccact ggttaatgcg ccaatagccg
    13681 caccgagcgg gatcgccgca tagagaaaac caatctctgc cgccgacatc tgccagttgt
    13741 cagccagcgc cggatacagt acccgcaccg cgctcgccat cgtcaataaa ccacccagca
    13801 acgcaatccc gcccaccagc gggctggcga gcagaaaacg aaatcctgcc agtaatgatt
    13861 tcaacggatg ctcgcgcggc tgcggtggcg gtggcaacgc cggaaggctt aacaacggta
    13921 gcaaggtaat aaacgtgccc gccgccgcca gcccgtagtt ccaggctacg ccaccggtcg
    13981 ccagcaataa accgccaatc atgggcgaaa tcaccgaccc cagacgcacg gtcaacatgg
    14041 tgatcgcccc ggcctgcatt aagttttcac gccctaccag tgctggtgtc gccgccaata
    14101 gcgccgtaac gccaagcgat gcgaaaaaac catcccataa accaagtaaa tagattgcca
    14161 gcaatgacgg ctccggcagc agtgcattaa ggcacagtcc aatgaagcca atgccacagg
    14221 tgccgcgcgc cagcaaaatc acttttttgc gctcatagcg atccgccagc acaccgccaa
    14281 ccatcaggcc aacaaacatc gcgccgccgg tcagcgtcac cgaaagcccc accagccagg
    14341 tagaatgcgt catcatctgg atctgcaccg gcaccgcgac gccgagcaaa cccagagaca
    14401 caattgagat aaaacgagcg aggaatactg cgcgaaacgc cgggtgcgtt ttcaacaggc
    14461 tgaggttaag cagccaggat tgtttattca ttacaatgcc ttgccatcaa atattttaaa
    14521 tacctattct taggctgcac atgctaacat atccaaataa gatcgataac gataattaat
    14581 ttcattatca tggaagttcg tatgtctggt tctgttgccg tgacacgcgc cattgccgtg
    14641 cccggattgc tgttattact gattatcgcg acggcattaa gcctgctcat tggggcaaaa
    14701 tcactccccg cttccgtagt gctggaggcc ttctccggca cctgccagag cgccgactgc
    14761 accatcgtgc tcgacgcgcg actgccgcgc acccttgccg gtttactggc aggcggcgcg
    14821 cttggccttg ccggggcatt gatgcaaacc ctcacccgaa atccacttgc cgaccccggc
    14881 ttgcttggcg tgaacgccgg agccagcttt gccattgtgc tgggtgcggc gctgtttggt
    14941 tactcatccg cgcaggaaca actggcgatg gccttcgccg gggcgctggt ggcctcattg
    15001 attgttgcct ttaccggcag tcagggcggc gggcagttaa gtccggtgcg tttaaccctg
    15061 gcgggcgtgg cgctggcggc agtgctggaa gggctgacca gcggcatcgc cctgcttaat
    15121 cctgacgtct acgatcaact gcgtttctgg caagccggtt cgctggatat tcgcaatcta
    15181 cataccttaa aagtggtgct gatcccggtg ctaatcgccg gagcaaccgc gctattactg
    15241 agccgcgcgc tgaacagttt aagcctcggc agcgacaccg cgacggcgct gggcagtcgc
    15301 gtggcgcgca cacagttaat tggtctgctg gcgattaccg tgctttgtgg tagtgcgaca
    15361 gcggtagttg gcccgattgc ctttattggc ctgatgatgc cgcatatggc gcgttggctg
    15421 gtgggtgccg atcatcgctg gtcgctgccc gtaacgctgc ttgctacccc tgccctgctg
    15481 ctgtttgccg atatcatcgg gcgggtgatt gtgcccggtg aactgcgcgt ttctgtggtc
    15541 agtgccttta ttggtgcacc ggtgctgatc ttcctcgtgc gacgtaaaac gcgaggtggt
    15601 gcatgattta cgtctctcgc cgattactca tcacctgttt gctgcttatt tccgcctgtg
    15661 tggttgcagg tatctgggga ttacgcagcg gtgccgtcac gctggaaacc tcgcaggtat
    15721 tcgccgcgct gatgggcgat gcgccgcgca gtatgacgat ggtggtcacc gaatggcgtt
    15781 taccacgcgt gctgatggcg ctgttgattg gcgcggcact gggcgtcagc ggtgcgattt
    15841 ttcagtcgct gatgcgtaac ccgctcggca gccctgacgt aatgggcttt aacaccgggg
    15901 cgtggagcgg cgtgctggtg gcgatggtgc tgtttggtca ggacctgacg gctatcgcgc
    15961 tggcagcaat ggtgggcggc attgtcactt ctctgttggt ctggctgctc gcctggcgta
    16021 acggtatcga cacctttcgg ttgattatta tcggcatcgg cgttcgcgcc atgctggtgg
    16081 cctttaatac ttggctgttg ctgaaagcgt ctttagaaac ggcgctaaca gcgggtttgt
    16141 ggaatgccgg atcgctcaat ggcctgacgt gggcaaaaac ttcgccttcc gcacccatca
    16201 ttatattgat gctcattgcc gccgccttgc tggtgcggcg aatgcgcttg ctggagatgg
    16261 gcgatgatac cgcctgcgcg ctgggcgtca gcgtcgagcg ttcgcgtctg ttaatgatgc
    16321 tggttgcagt ggtgcttacc gctgcggcaa cggcgcttgc cgggccgatt tcctttattg
    16381 ctttagtcgc gccgcacatt gcccgacgca ttagcggcac cgctcgctgg gggctgaccc
    16441 aggcggcgct gtgcggagcg ctgttactgc tggcggccga tctctgcgcc caacaactgt
    16501 ttatgccgta tcaacttccg gttggcgtcg ttaccgtcag cctcggcggt atttacctta
    16561 tcgtcttgtt aattcaggag tctcgcaaaa aatgaccgaa tcagtagccc gtttgcgcgg
    16621 cgaacagtta accctgggat atggcaaata taccgttgcg gaaaatctga ctgtagaaat
    16681 tcctgatggt cacttcacgg caattatcgg accaaatggc tgtggtaaat ccacgttact
    16741 gcgtacctta agccgcctga tgacgcctgc tcacgggcat gtctggctgg atggcgagca
    16801 cattaaacat tacgccagta aagaagttgc acgccggatt ggtctgttgg cgcaaaatgc
    16861 taccacgccg ggcgatatca ccgtgcagga gctggtggcg cgtggacgtt atccgcatca
    16921 accgctgttt acccactggc gcaaagagga tgaagaagcg gtaacgaaag cgatgcaggc
    16981 cacgggaata actcatctgg cagatcaaag cgtggatacc ctttctggcg gacaacgcca
    17041 gcgagcgtgg atcgcgatgg tgctggccca ggaaacggca attatgctgc tcgacgaacc
    17101 cacgacctgg ctggatatca gtcatcagat tgatctactg gaactgttaa gcgaactgaa
    17161 ccgcgagaaa ggctataccc tggcggcggt gctgcacgat cttaatcagg cctgtcgtta
    17221 cgccagccat ttgattgcat tgcgggaagg gaaaattgtt gctcagggag cgccgaagga
    17281 gattgtcact gctgaactga ttgagcgcat ttatggtctg cgatgcatga tcattgacga
    17341 tccggtagcc ggaacgccgc ttgtggtgcc gctcgaacga acggcacctt caaccgcaaa
    17401 tagttaaact aagtggtctg ccatcatggc atcctgtttt ctggatgcca tcgcatagcg
    17461 caataacacg ctaccacaag ccaccatccc gccgatcagc gcggaaagga tcacaataat
    17521 cgccttaccc ggaccgtctt ttttcactgg caatgacggg cttaactgat atttaaacgg
    17581 cgtaaaattc acatcgttga catttgcttt tgttaattgc tcgacaagat actgccgatt
    17641 acgtaattca ccgttcagtt ccgcaacgtc agtaaccgct ttttcaattt ccagtttgcg
    17701 ttcaataccg tctgcgccga gagaaataga gaaatcgggg tcatctttaa cggcctgacc
    17761 attactgtat acgggctttt taattcctgc cgcgttggca atgtcgagtg aataattgag
    17821 gcgctgaatg tttgcatcaa gttgattttt cgttttaatg cgatcctgag ccagtttttc
    17881 tttttcaaac tgggttttga tctccagttt attacggacg ttttctatcg actctttcac
    17941 caccaacgca gagatataat cgatataccc gctcaaaacg gtctgcgcct ctttactggt
    18001 tggcgcggta aaacttagcg tccaggaggt atacagtgac ggttcatctt ttttcttact
    18061 ggcattgtca tcaaccgctt tcattttttc gctcaatgca acaatagcgc gatgcaaatc
    18121 cagttcatcg attttcgcct cttttaattg gtccatcaca taaggtgatg aacgcaggta
    18181 ctcttccagc aagctaacag actgaaactt cttgataaac aggttaaatg caccgccacg
    18241 gtcgatatta acatcgagat caagaacgcg gagtttggta aaggttttct ccaggtcctg
    18301 ccactgaatg gcttcggcgg gagtaataac ggctgagctg gtccattttt gcggcaggat
    18361 gaaagagatc agtaaacctg cgcaggcaaa tgcaaaaacg accgccatga ccgttttttt
    18421 ggcccgccat aaaacttcga ttagattaag taaatcaatt tcattactac tgggcgacgc
    18481 cagaggatac tcggaaaaat gagcgtcact tccctgttta atattcagtg atgacatggg
    18541 tataacctga aaatgagtcc aataggggga aatctccacg gcgctcactt taccagttgg
    18601 tgagaattat ccgaagctga agtttgtaaa ttccttccac tgaatgcaac attcactcta
    18661 tttaagagaa atattcatca aacctataaa tcaaacaaat aaaaaaccca taattatttt
    18721 ctgtaattat gggtttatag ataatattaa tttacctgtt tagcgttgcg cgaataatcg
    18781 gcccaatttt tacaaacgcc cctggagaga taatatccac atgcgcacaa tcctgacgat
    18841 agatatccag ctcggcaatc cagggcgacc aagcgcgttc ggggctcata ccttcctgaa
    18901 gcgtgcgttc agcaacaaac agcgtcgctt taccatcaaa cggtacgcta tgagcagtcg
    18961 tcagcagacg cacagcatca gcgtagttgc cttcaatggt ggtaaacaac tccgttgaag
    19021 tacttccctg ctgtgccgcc aggaaggcct cgcgttcgcg gttaatctcc gccagcactt
    19081 ccgggtccag accattagcc tctttttcct gccagttttg cgtttctggc ggccaggtat
    19141 ccagcaaacc aagaaatgcc acctgttcgc cacgggcacg cagtcgcgcc gcaatgccct
    19201 gcgccagcgt accgccaagg gaatacccca gcaggtaata agggccgtgc ggttgttgtt
    19261 caagtaacgt tgccagatgc gcttcacaga cttcatccag atttgccgcg gtatgcatgg
    19321 ggccattggg gcgcggcgac tgaatgccga taatcgacca ttgtggatcg atataacgcg
    19381 agagcacgct aaactgccag gcaaaaccgg atgccggatg gaaacaaaac agcgtcgggc
    19441 cattaccttc acgcaacggc agaatggttt cgaatcccat acgccgggtg ctgtcttctt
    19501 cagcatcaat aatcgttgcc agtttagcga cggttgacgc gaccatcact tgccccggcg
    19561 tcacctggcg ggcaacctgc cgacttaact gcgctgccag tttcattgcc agtagcgaat
    19621 gaccaccaag cgcaaagaaa tcagcatcgg catcctgcac gtcacaaccc agcaacgacg
    19681 cgaatgccgc ggcaataatc gtttcactgc ccgcttttgg cgcacgcccc ggcgcttgtg
    19741 ccttcagttc aggcaacggt aaggctttgc gatccagctt gccgttggcg ctaagaggta
    19801 acagtggaag ttgcagcaga accacgggca ccatatgtgg tggcaatgtt tcgcgaagct
    19861 gcgcctgtaa tgcgctggta tccaacggca gacctgattg cgacaccaga tagcccacca
    19921 actgacgcgc atcaccaccg gtggctgccg cctggttaat cacacaggcg tgggtaacgg
    19981 cttgttcgac atccggcagc gcctgcatca cgcgatcgat ttcgcccagt tcaatacgct
    20041 gcccgcgaat ttttagctga tcgtcactgc gcccgaggta ctccaccgcg ccgttatcca
    20101 gccagcgggc aacgtctccg gtacggtaca tccgttcacc agggacaaaa ggatcggcaa
    20161 taaagcggct ggcggtcaga tcgggtcgtc caagataccc ctgcgccagt tgaatgccgg
    20221 tgagatagag atcacccgcc acacccggcg gcaccggatg catcatcgca tcaagaatac
    20281 gcagacccgt attccatacc ggataaccaa tcggcacact gctgccgcgc acctgtgcca
    20341 gttcctcgcc aaaagccgga taccagctga catctaccgc cgcttccgtc gggccatata
    20401 gattatgcaa cggcacgctg gttaactgtt gccattcgcg gcataaatca gccggtaagg
    20461 cctcaccact acagaaaacc tgtttcaacg tcgcgcaact ctggcgagcg gtttgcggcg
    20521 tcagcgatgc aacaaatgct gccagcatcg acggcacaaa gtgcgtggtc gttacgccat
    20581 attcggcaaa gaattgctgc atagcgagcg ggtcgcggtg cgcttccggt tcagccatca
    20641 ccagtttagc cccggcaata aacggccaga aaaactccca caccgagaca tcaaaactgc
    20701 acggcgtttt ttgggcaacg acatcttcgc ctgtaagcag ataatgattt tgcatccaca
    20761 acaggcggtt aacgatagcc gtctgcccga ccattacccc tttcggcctg ccggtggagc
    20821 cagaggtaaa gatgatataa gccgtgtgat gcggttgtga aagttgcagc ggcgcactgc
    20881 cctgcggttt aagcggggcg ttatagcaaa ggttggttaa atctggaaca tcggcaaagc
    20941 gcggcagttg atcgtcggtg gtgattaaca gcgacggacg cgcatcttcc agcatcattt
    21001 tcaggcgatc gtccggatag ccggtatcca gcggtagcca ggccgcgcca gcttcaacta
    21061 tcgcatgtag cgccagggtc aaaaagaccg agcgcggtag cgccaccgcc acgctgtcgc
    21121 ccggtttaac gccacgctca cgcagcagat tcgccagcgc caccacctgc tcgcgcattt
    21181 cccgatagct gaactggtaa cgcgcatctg ccagcgccgg agcatccggt gtttttgccg
    21241 cttgttctgc caccagcgcg ctcagcgtgg tttctggaat ctcaacctga gtggcgttga
    21301 tctgcgccag ctgcgcatac tcacctggca gcataatatc gacatcgccg cacaacagcg
    21361 ccggatccgc ggcgaactgg gcaatcagca ttttcaggcg ttcagcatgc tggattaacg
    21421 ttggctcatc gtaacgctgt ttattggcga ggatctcaat actcaaatca ccgtgttcat
    21481 ccgggaacag ggccagttca aggtcattaa ccggaccggt tgccaaggta tgggtttgcg
    21541 cctgaacacc aggaatatcc agttggtaat caaatacctt gatattgagt accggaccaa
    21601 acagcggttc atcacctgcc gctcgcccgc tgtcacggac aatttgttcg gcatcgtaac
    21661 gttgatgacg acgcattttt ttcagttgtg ctgccagtcg ggttgccagt tccggcagcg
    21721 tttcttgcgc cgcaatgtga atacccaacg gcaaaacgtt gagcacgggt ccggtagccg
    21781 tcagcgccgc cgagcccagt cgacgcataa agataaatcc agcggcgtag tccatgcgat
    21841 tgcacaatcg ccccagccac aaagccgtca gcgcaagggc taaatcggta cgctgcacac
    21901 ctgaaagttg cgtagccaac tggcggaatt ccccgtcggt aaattccatt ttcagacgta
    21961 aaatatctgc cgaagcgctg cgccccggta aaggtgccgg agaaagtgac gcgggcggcg
    22021 gcagttgacg acgctgttct gcccagaatg ccgcatcacg ctgccaggct tcgctttcgc
    22081 ggtattgctg gtactcttcc actacatcag cgaaaggcgt aaacggcgaa gcaggcgttg
    22141 gttcaccacg cagccatgtg cagtaaatat tggcgatctg gcgggtaatg gccgggaaac
    22201 tgaagccatc gaccagcaaa tggtgataac gctgatacca gtaccagcgg ttatccgcca
    22261 cctgtatcag ctgatgaaag accagtggtt taccgctatc gacgcgcaga tcttgttgca
    22321 aatccgcctg cattaatgcc tgcgcagtac cgtgcggatc aatgttggtt cgcaggtcga
    22381 taatttctgg cagttcgaac gtcagcgcat catcgaccca ctgccagact tcgccgttat
    22441 cttccgtaaa acgcatccgc agcgtatctg cttgcgctag tccgtcaacc accgcgcggg
    22501 ccagtaatgg cgcatcaacc tctccggtta actcaacgta atgcgctacg ctccaggcgg
    22561 agggtaaatc cgacagtttt tctgccatcc agatgccggg ctgtgcggcg accaaaggta
    22621 aatgctggct cattgcgcct cctgcaactg ggtgaaattc gccggtgtca gagtacgcca
    22681 gtgggcttca agccactgct ggcaggactc ttgtgactgc ggctgacaca caatgtccca
    22741 gcctgccggt aaggcgcatt gttgcggcca cagactgaat tgcccctgcg cattgcgcaa
    22801 aatgtaaaac gctccctgtg gatcatcgaa gggattactg aatgccatat tcaactcctg
    22861 tcatggaaaa gtggttgcca gaggtcgatt agcccctgca tcaagccacc gcgccaacaa
    22921 agcgcatcat gtccgccgtc aacctgacgc cagaaaatgg attcttttat ggggtgtaat
    22981 tgcgcataca gcgcctgatt ggcccgcata atcatcggct cgcgaatacc cgcttccagc
    23041 acaatgcgca gaccttcggc gctaacttca ccagctttaa gcttttcaag taacacgccc
    23101 tcttgctgcc cgccccgatg cggccaccag tacgatcctg actggcttaa tacacaacca
    23161 aaacgttcag gccagtgcag tccggcatac agcgcggaaa gtccaccaaa actctgcccg
    23221 gcaaccacgg tgcgatcggc gcgatcgcta aaaggggcaa tagctttcac caggggtaat
    23281 aactcttgct gtactgcgag ccagaaatcc gcattacacg gcagttcgtg agcgcggtgc
    23341 gtggtgtcga tagcgtcgat caacacatac acggcgggag gaagttgctg acgatgggtc
    23401 agcgaagtca gcactggcca gacgggcata ctttgcgccc aaaattcgcc atcgagcaaa
    23461 actgccagcg ggcgttcttc tgctgttgca tcgccggtgg taaaaatcca tacacgacgt
    23521 gaatttttca accgttcact tttccagata atttctttgg ctggtatttc tggcgcttgc
    23581 ggacaatccc atcccggttg cagaggcgct tgcggcattt cgagtgcaga aacagcgtgc
    23641 cctcgcccgc ctttccagct ttgtggattc agcggatcgg ctattgcctg gggtaatagt
    23701 tttcgccagc cttcacgtaa ttcgaggcga tcgggggatg gtgcagaaaa aatgtcatcg
    23761 cgttcggtgg gaataaagca gtagctgccg cgccagttgg cattgagttg tgtcgtccac
    23821 tgccagacat cagtacctgc aatgcgctgc atcgactggg gctggctgtt ctgatgatga
    23881 tcggtcacac cagtgatgta gacccatacg cgctttatcg gcgagtattc ttcagaacct
    23941 tggggatcac gccaccagaa agtgacctca aacatttcgt cattcagacg ctgccattcc
    24001 gggccatgtt tcgactgcca ccagctctca cttcctactt ttaacgccgt cacaccataa
    24061 ccccatgttt actgtgcaat ttttcattga ttgcagaaat atattgataa tattattgat
    24121 aactatttgc atttgcaata gcgtaatggc gcgccgtggg aagcgcggac attaattaac
    24181 caactgcact gcgtgtcttt caggatcaaa ggttttcgcg gtagcgggat gcgtcgtgtt
    24241 gaggacgacc atgcccgaca gttgcaattc gtggcaaaaa tgcaggaata aaacaatgaa
    24301 caagaagatt cattccctgg ccttgttggt caatctgggg atttatgggg tagcgcaggc
    24361 acaagagccg accgatactc ctgtttcaca tgacgatacc attgtcgtta ccgccgccga
    24421 gcagaacttg caggcacctg gcgtttcgac catcaccgca gatgaaatcc gcaaaaaccc
    24481 ggttgcccgc gatgtgtcgg agatcatccg taccatgcca ggcgttaacc tgaccggtaa
    24541 ctccaccagt ggtcagcgtg ggaataaccg acagattgat attcgcggta tgggtccgga
    24601 aaacacgctg attttaattg acggcaaacc ggtcagcagc cgtaactcgg tgcgtcaggg
    24661 ctggcgtggc gagcgcgata cccgtggtga tacttcctgg gtgccgcctg aaatgattga
    24721 acgtattgaa gttctgcgtg gtccggctgc agcgcgttat ggcaacggcg cggcgggtgg
    24781 cgtagttaac atcattacca aaaaaggcag cggtgagtgg cacggttcct gggacgctta
    24841 tttcaatgcg ccagaacata aagaggaagg tgccactaaa cgcaccaact tcagtctgac
    24901 cggcccgctg ggcgacgaat tcagcttccg cttgtatggc aacctcgaca aaacccaggc
    24961 tgacgcgtgg gatatcaacc agggccatca gtccgcgcgt gccggaacgt atgccacgac
    25021 gttaccagcc gggcgcgaag gggtgatcaa caaagatatt aatggcgtgg tgcgctggga
    25081 cttcgcgcct ctgcaatcac tcgaactgga agcgggttac agccgccagg gtaacctgta
    25141 tgcgggcgat acccagaaca ccaactctga cgcttacact cgctcgaaat atggcgatga
    25201 aaccaaccgc ctgtatcgcc agaactactc gctgacctgg aacggtggct gggataacgg
    25261 agtgaccacc agcaactggg tgcagtatga acacacccgt aactcgcgta ttccggaagg
    25321 tctggcgggc ggtaccgaag ggaaatttaa cgaaaaagcg acacaggatt tcgtcgatat
    25381 cgatcttgat gacgtgatgc tgcacagcga agttaacctg ccgattgatt tcctcgttaa
    25441 ccaaacgctg acgctgggta cggagtggaa tcagcaacgg atgaaggact tgagttccaa
    25501 tacccaggcg ctgaccgggg cgaacaccgg cggcgctatt gatggtgtga gtgccaccga
    25561 ccgtagcccg tattcaaaag cagaaatttt ctcgctattt gccgaaaaca acatggagct
    25621 gactgacagc accatcgtaa cgccggggct gcgtttcgat catcacagta ttgtcggcaa
    25681 taactggagc ccggcgctga acatatcgca aggtttaggc gatgacttca cgctgaaaat
    25741 gggcattgcc cgcgcctata aagcgccgag cctgtaccag actaacccga actacattct
    25801 ctacagtaaa ggtcagggct gctatgccag cgcgggcggc tgctatctgc aaggtaacga
    25861 tgacctgaaa gcagaaacca gcatcaacaa agagattggt ctggagttca aacgcgacgg
    25921 ctggctggcg ggcgtcacct ggttccgtaa cgattatcgc aataagattg aagcgggcta
    25981 tgtggctgta gggcaaaacg cagtcggcac cgatctctat cagtgggata acgtgccgaa
    26041 agcagtggtt gaaggtctgg aaggatcgtt aaacgtacca gttagcgaaa cggtgatgtg
    26101 gaccaataac atcacttata tgctgaagag tgaaaacaaa accacgggcg accgtttgtc
    26161 gatcatcccg gagtacacgt tgaactcaac gctgagctgg caggcgcggg aagatttgtc
    26221 gatgcaaacg accttcacct ggtacggtaa gcagcagccg aaaaaataca actacaaagg
    26281 tcagccagcg gttggcccgg aaaccaaaga aatcagtcct tacagcattg tgggtctgag
    26341 cgcgacctgg gatgtgacga agaatgtcag tctgaccggc ggcgtggaca acctgttcga
    26401 caaacgtttg tggcgtgcgg gtaatgccca gaccacgggc gatctggcag gggccaacta
    26461 tatcgcgggt gccggggcgt atacctataa cgagccggga cgtacgtggt acatgagcgt
    26521 aaacactcac ttctgatgct aacgtcagat tgttgataaa gtgcgcgtcg ttcatgccgg
    26581 atgtggcgtg aacgccttat ccggcctaca aaaatcttgc caattcaata tattgcagga
    26641 ccatgtaggc ctgataagcg tagcgcatca ggcagtttag cgtttgtcac cagtctctaa
    26701 tatggtcgac atgaaaacta cgcatacctc cctccccttt gccggacata cgctgcattt
    26761 tgttgagttc gatccggcga atttttgtga gcaggattta ctctggctgc cgcattacgc
    26821 gcaactacaa cacgctggac ggaagcgcaa agccgaacat ttagccgggc ggatcgctgc
    26881 aatttatgcg ctgcgggaat atggctataa atgtgtgccc gcaatcggcg agctacgcca
    26941 accggtgtgg cctgcggagg tatacggcag tattagccac tgcggggcta cggcattggc
    27001 cgtggtttcc cgtcaaccga ttggcgttga tatcgaagaa attttttctg cgcaaacagc
    27061 gaccgaattg acagacaaca ttattacacc agcagaacac gagcgactcg cagactgcgg
    27121 tttagccttt tctctggcgc tgacactggc attttccgcc aaagagagcg catttaaggc
    27181 aagtgagatc caaactgatg caggttttct ggactatcag ataattagct ggaataaaca
    27241 gcaggtcatc attcatcgtg agaatgagat gtttgctgtg cactggcaga taaaagaaaa
    27301 aatagtcata actctgtgcc aacacgatta attgacaaca tctggtacga ttcgcccgca
    27361 gccatcactg accgcgggcg aaagtgtaaa gcaggtgcct taccatcctg acctgacaac
    27421 cggatatgcg gggattgctc cccgcacaac ggctacttct tcggttcgta agcgagaaca
    27481 gccttaaact caatattacg ttcctttacg gtgaactcac acagcgaatc cccgaccaga
    27541 agcgtaaatc ccagcaccgt taaacacagc actatgactg ccgcaagggc atatttcgtc
    27601 agcatgttta tattgcctcc atgcgaagaa gggactacca tccaaattgt tcaggtttgg
    27661 gtaggtagcc ccagggttga tagaaatatc gtcctggggc ttcatctttc cagacctcag
    27721 agtaaacctg aaaccggaaa gcttcaggca cccgcagtta tcttaccctt gcttagcaaa
    27781 atagctgttt atttatacag taagtcctgt ttcggacttc tcactcccca tttgatcaac
    27841 tgaagctgaa cccgtttttt tctgcatgcc tcaatcgtca aaattcttcg ccaccagtca
    27901 gcactgccat aacttgtcac attgcaaaag tagccaaatt gcttctgtca taatcagttc
    27961 accatcacca aaaaaaagaa acattccaga acttccgcat ctcgcccagc cgctcccact
    28021 tctatactga atagaaaacg ccaacataag agaaacctat gccattaccc gattttcatg
    28081 tttctgaacc ttttacactc ggtattgaac tggaaatgca ggtggttaat ccgccgggct
    28141 atgacttaag ccaggactct tcaatactga ttgacgcggt taaaaataag atcacggccg
    28201 gagaggtaaa gcacgatatc accgaaagta tgctggagct ggcgacggat gtttgccgtg
    28261 atatcaacca ggctgccggg caattttcag cgatgcagaa agtcgtattg caggcagccg
    28321 cagaccatca tctggaaatt tgcggcggtg gcacacaccc gtttcagaaa tggcagcgac
    28381 aggaggtatg cgataacgag cgctatcaac gaacgctgga aaactttggt tatctcattc
    28441 aacaggtgac cgtttttggt cagcatgttc atgttggctg cgccagtggc gatgacgcca
    28501 tttatttgct gcacggcctg tcacggtttg tgccgcactt catcgccctt tccgccgcgt
    28561 cgccatatat gcaaggaacg gatacgcgtt ttgcctcctc acgaccgaat attttttccg
    28621 cctttcctga taatggcccg atgccgtggg tcagtaactg gcaacaattt gaagccctgt
    28681 ttcgctgtct gagttacacc acgatgatcg acagcattaa agatctgcac tgggatattc
    28741 gccccagtcc tcattttggc acggtggagg ttcgggtgat ggataccccg ttaaccctta
    28801 gccacgcggt aaatatggcg ggattaattc aggctaccgc ccactggtta ctgacagaac
    28861 gcccgttcaa acatcaggag aaagattacc tgctgtataa attcaaccgt ttccaggcct
    28921 gtcgctatgg gctggaaggc gtcattaccg atccgcacac tggcgatcgt cgaccgctaa
    28981 cggaagacac cttgcgattg ctggaaaaaa tcgccccttc cgcacataaa attggtgcat
    29041 cgagcgcgat tgaggccctg catcgccagg tcgtcagcgg tctgaatgaa gcgcagctga
    29101 tgcgcgattt cgtcgccgat ggtggctcgc tgattgggct ggtgaaaaag cattgtgaga
    29161 tctgggccgg tgactaagta gggaattgcc ttacagaaga atatccctga caatggtgtt
    29221 ttaacttaca tttaacaatg ttatgaaaca ccctttagaa accttgacta ccgcagcagg
    29281 cattttgctg atggctttcc tctcttgcct gctgctgccc acccccgcac tggggctgac
    29341 gctggcacaa aaactggtga ccatgttcca tctgatggat cttagccagc tttacacttt
    29401 attgttttgt ctgtggtttt tagtgctggg cgctattgag tattttgttc tgcgctttat
    29461 ctggcgacgc tggttctcgc tggcggatta aacgtcgatt atcgccgtac ttcgcggcat
    29521 actttgctta ttctccttcg cctaaaggaa cgtttatgga taagcaatca ctgcacgaaa
    29581 cggcgaaacg cctggccctt gagttaccct ttgtcgagct ttgctggcct tttggcccgg
    29641 agttcgatgt ttttaaaatt ggcggcaaaa tttttatgct gtcgtcggag ctgcgcggcg
    29701 tcccctttat caatctgaag tccgatccac aaaaatccct gttaaatcag cagatatacc
    29761 cgagcattaa gccagggtat cacatgaata aaaagcactg gatttcggtg tatcccggcg
    29821 aggaaatctc cgaagcgtta cttcgcgatc tgatcaacga ttcgtggaat ctggtggttg
    29881 atggtctggc taaacgcgat caaaaaagag tgcgtccagg ctaaagcgga aatctatagc
    29941 gcatttttct cgcttaccat ttctcgttga accttgtaat ctgctggcac gcaaaattac
    30001 tttcacatgg agtctttatg gatatcattt ctgtcgcctt aaagcgtcat tccactaagg
    30061 catttgatgc cagtaaaaaa cttaccccgg aacaggccga gcagatcaaa actctcctgc
    30121 aatacagccc atccagcacc aactcccagc cgtggcattt tattgttgcc agcacggaag
    30181 aaggtaaagc gcgtgttgcc aaatccgctg ccggtaatta tgtgttcaac gaacgtaaaa
    30241 tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac cgcgatggac gatgcctggc
    30301 tgaagctggt tgttgaccag gaagatgctg atggccgctt tgccacgccg gaagcgaaag
    30361 ccgcgaacga taaaggtcgc aagttcttcg ctgatatgca ccgtaaagat ctgcatgatg
    30421 atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg taacttcctg ctcggcgtgg
    30481 cggctctggg tctggacgcg gtacccatcg aaggttttga cgccgccatc ctcgatgcag
    30541 aatttggtct gaaagagaaa ggctacacca gtctggtggt tgttccggta ggtcatcaca
    30601 gcgttgaaga ttttaacgct acgctgccga aatctcgtct gccgcaaaac atcaccttaa
    30661 ccgaagtgta attctctctt gccgggcatc tgcccggcta tttcccctca gattctcctg
    30721 atttgcataa ccctgtttca gccgtcatca taggctgctg ttgtataaag gagacgttat
    30781 gcaggattta atatcccagg ttggggattt agcgggtatt gagatcgatc acaccacctc
    30841 gatggtgatg attttcggta ttatttttct gaccgccgtc gtggtgcata ttattttgca
    30901 ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc agttcacggc tttggttgca
    30961 aatcattacc cagaataaac tcttccaccg tttagctttt accctgcagg ggattatcgt
    31021 caatattcag gcggtattct ggctgcaaaa aggcaccgaa gcggcagata ttctgactac
    31081 ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca gtcttctcgt tgctggatgt
    31141 tattttgaat ctggcgcaga aattcccggc ggcatctcag ttaccgctga aagggatatt
    31201 tcaggggatt aaactgatcg gcgcaatcct ggtcggcatt ttgatgatct cgctgctgat
    31261 tggtcagtcg ccagcgattc tgatcagcgg tcttggtgca atggctgccg tgctgatgtt
    31321 ggtatttaaa gatccgattc ttggtctggt ggcaggtatt cagctttccg cgaacgatat
    31381 gctgaaactg ggcgactggc tggagatgcc gaaatacggc gcggatggcg cggtgatcga
    31441 tattgggtta accaccgtca aagtgcgtaa ctgggacaat accattacca ctattcccac
    31501 ctggtctctg gtttctgact cctttaaaaa ctggagcggg atgtcagcat ctggcgggcg
    31561 acgtattaag cgcagtatca gtattgatgt caccagtatt cgttttcttg atgaagacga
    31621 aatgcaacgt ctgaataaag cgcacttgtt aaaaccttat ttaaccagcc gccatcagga
    31681 aattaatgag tggaatcgcc agcaaggttc tacggagtcg atattaaacc tgcgccgaat
    31741 gaccaatatt ggaacctttc gtgcctatct gaacgaatat ctgcgtaacc atccgcggat
    31801 tcgtaaagat atgaccttaa tggtacgcca actggctcca ggtgataacg gtttaccgct
    31861 cgagatctac gcgtttacca acaccgtggt gtggctggaa tatgaaagca ttcaggctga
    31921 tatattcgat cacatatttg cgattgtcga agagtttggt ctgcgacttc atcagtcgcc
    31981 agccggcaat gatattcgct ctctggcggg tgcatttaag cagtaattaa aaaaaccgct
    32041 ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg tagcgtgaat gccttatttc
    32101 cgacgcagcg ttttaaatgc cacaaacagg aatacaatcc acaccggcag caggatcgct
    32161 gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatgccgag gaaggcaatg
    32221 caaagatagt tgccgaacgg ataaagcagc gccttaaact gtgtttcacg tccctgacgt
    32281 cgcatcgccg cgcgaaaacg cagatgcgcc aggcagatca taatccagtt caacagcagc
    32341 gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag atagttgatt
    32401 aacaccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg cacgccgcga
    32461 tggctgacgc gagtcaaaaa cttcggcgca ttaccctgca cagaaaggcc aaacagcatg
    32521 cggctgttag agtaaacccc gctgttatac actgacagcg atgctaccag aatgacgaag
    32581 ttcagcgcag aagctacctc gttgctgtcg agattatgga aaatcatcac aaacgggcta
    32641 ctgttggatt tcacttccac ccacggatag agcgccagta aaaccaccag tgaacagatg
    32701 taaaacagca ggatgcgata caccacctga tttaccgctt ttggaatgct tttttccgga
    32761 tcgcgcgctt cagcggcagt aatcccaatc agctccagac cgccgaagga gaacataatt
    32821 accgccagcg acaaaatcag cccattccag ccggtggcga agaaaccacc gtagcgccag
    32881 aggttgtcga tactggcttt ctcgccgccg tgaccagaaa acagcagcca caggccaaag
    32941 ccgatcatac cgatgattgc cagcacttta atcaacgcaa accagaactc ggtttcgcca
    33001 tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc ggcagcccaa
    33061 atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc ggtcagctct
    33121 gccattccca ccagcacgaa cattacccag tagttccagc cagagaggaa gcccgcaaac
    33181 ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc ttcgaccacc
    33241 atctcgccga gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc gtagcccagc
    33301 aatacagccg gacccgccat ctgaatcgcc gggccaatgc caagaaacag accagtacca
    33361 attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg atgaagcgtc
    33421 ggctcttgat ttgacgcagt atcttccgat acggttgacg cgtttttcac gcctttcccc
    33481 tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt cgcaagacac
    33541 aatccacacg gttaaacggg gtatcctgct tttatttccg tacccgatgt cggtgcagcc
    33601 acatcagctt atacgccgcc gggataataa acagcgacag caaaggcgcg gtgatcatgc
    33661 cgccaatcat cggtgcggca atccggctca tcacctctga accagccccc gttccccaca
    33721 gaatcggcag cagaccggcg ataatcaccg ccaccgtcat cgctttcggg cgcacgcgca
    33781 gtaccgcgcc gtgatacaac gcctcatcca gcttctgctc actgaatgtt tgcggattat
    33841 tcaacgacgg ctcggcctct atggcgtgac gtaaatacat cagcatcacc acgccaaatt
    33901 cggcggcgcc cccggcaagg gcaataaaac cggtgcccgt tgccacagaa agatgaaagc
    33961 ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg ctgataatca
    34021 gcaacgcttc gcccacccga cggaatgcca gatacaacag cacgaagatg atcatcagtg
    34081 tcatcggcac catgagtttc agcttatggt tggcgcgctc cagtagctcg aactgcccgg
    34141 agaatgccac gctggtgccc ggttttaact gcactttttc aactatcgct ttttgcaaat
    34201 cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag ctcgtcgggc
    34261 gcgcattctc ggttttcagc atcgacggtc cggtagagac tttaacgtcg gccacgtctg
    34321 ccagggtgat ttgctgcttc atcggcgtca ggatcggtag ctggcgcagt gcctgctttg
    34381 cggataacgc agattaatgg gataacgggc aatcccttcc accgtttcgc caaccatcgc
    34441 cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgcgc
    34501 ggctttttca cggttaatct caacgttgat atagcgccca ccttccagac gctcggcaag
    34561 cgcagaagcc acgcctggca ccgttcgcgc tacttcttca atttgctcag ccatcgcgtc
    34621 gatatccgcc agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga
    34681 gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagccg
    34741 cacggtgtta tccagttcct caatgatttt gtccatcgtc atgccaggcc gccactgatc
    34801 ctgcggctta agctggatgg tcgtttcaac catctcgagc ggagcagaat cggtggcggt
    34861 ttccgctttc ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt
    34921 gtcgtttttt tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg
    34981 catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag
    35041 cggccagaga accgtcagca ccgaaagcgc tgccaccaac agcgtggttt tcggccagtg
    35101 cagtactttc agcaatagag gatgataaac acgaatcaaa aagcgattga gcgggttact
    35161 gctttccggc ggaattttgc cacggatcca gtaacccatc aggatcggga tcactacgat
    35221 cgccagcagc gccgcacccg ccatcgcata cgttttggtg aacgccagcg ggccaaacag
    35281 acgcccttct tgcccttcca gggtgaagat tgggataaac gacagcgtga taatcagcag
    35341 actgataaac agcgccggcc ccacttcaac agatgcatcg gtaatcacct gccagcgcgt
    35401 tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc
    35461 attctcgatc atgacgatag cagcatcgac catcgccccg acggcaatcg caatgccacc
    35521 caacgacata atattggcat tcagtccctg gaagggcatg acaataaaag caatacacaa
    35581 ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc
    35641 acagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcgatggc
    35701 gcggtcaatg agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact
    35761 acttttcagc gtttccagtt tgtccttcac ggcggcgatc acttctcggg cgtttttacc
    35821 ggagcgcagg atcaccaccc cgcccgccac ttcgccttcg ccgttcagtt cggcaatgcc
    35881 ccggcgcatc tccgggccaa cctggacctt cgcaacatcg cgcagataaa cgggcacgcc
    35941 attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct
    36001 ggcgcgcacc atatattccg cttccgccag ttcgatcgac gaaccgcccg cttcctggtt
    36061 tgaagcatcc agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg
    36121 gggatcgata accacctgat actctttcac cacaccgccc accgacgcca cttccgcaac
    36181 gtcagggatg gttttcagct catatttgag aaaccagtcc tgtaatgagc gtaaatcggc
    36241 cagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt
    36301 ggcatctggc cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag
    36361 gtactccagc acccgcgaac gcgcccagta cggatcggta ccatcttcga aaatgacata
    36421 cacataagag tcgccaaact gcgagaaacc acgcacagtc ttcgcgccag gcaccgacaa
    36481 catggtggtg gttagcggat aagtcacctg attttcaacg atttgcggtg cctgcccggg
    36541 atagctggtt ttaataatca cctgcacatc ggagagatcc ggcagcgcat ccactggcgt
    36601 attaatgatg gtccaggtgc cccagatgct aagaaacaac gcgcccatca gcaccaaaaa
    36661 acggttcgcc accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca
    36721 tgggtagcac tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca
    36781 atcaggaaaa ggccgctgga aaccaccttt tcaccttccg ccagaccaga gcgtaatgcg
    36841 gtgacacctt gcgatgcctg gaaaacggca acgcgtttcg gtacaaagcg cccgtcggca
    36901 tcaatggtaa tcacccgctg ttcgctaccg gtatcaatca gcccttgtga cggaatgagc
    36961 agcatcggtt cgctggcggt attgagttgc aaccaggcgt tcatgcccgg ttttagcgcc
    37021 tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgccg
    37081 ggtagcagcg tccatttgcg gatggtgagt gttttatccg gtcgtgccgg aacggtgagc
    37141 gtaaactgcg aggcatcttt caccagccag gcgatagact ccgggatcgc agcagtgacc
    37201 cacaccgggt ccataccctg aattttcgct accacgttat ctttggcgat attcattccc
    37261 gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg ctttgagcgt aaagcgagtc
    37321 tggatttttt gcgtggcgat caggcggcga atatccgcct ccggcattcc cgccagtcgc
    37381 agccgctcaa gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg cagcagtaaa
    37441 tactcactct gcgcttccac ccagtcagga atggtcaggt cgagaagcgg tgtgcccttt
    37501 tgtactttat cgcccacggt aagcggatac accttgtcga taaacccggc agcgcgagcc
    37561 tgcacaatgg catactgata ctcgttgtaa ctgacattcg ccgggaaact ctgggcaaaa
    37621 gtcagcggtc cgcgcgtgac ggtagccgtt ttcaccccca gattctgcgt ctgagtcggg
    37681 tcaatgcgca caccagacgc agaactctct tcatcggcat atttcggcac cagatccata
    37741 tccataaacg gcgatttacc tggtttatcg aaccgcgtat tggggtacat cgggtcgtac
    37801 cagaataaga ttttacgt
//
LOCUS       DAJTOR010000016        37615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTOR010000016 DAJTOR010000000
VERSION     DAJTOR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.016"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1988
                     /locus_tag="OO249_000755"
     CDS             <1..1988
                     /locus_tag="OO249_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCR5635151.1"
                     /translation="GVGAFLAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRD
                     TFNTEQVEVIKGPSGTDYGHSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDV
                     NQVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQH
                     NTPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHD
                     INDNTTIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKIL
                     TNQTNLTSTFYTGSIGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLT
                     RNGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITC
                     PAGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQ
                     SGSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQ
                     YGKKRVEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTL
                     WSQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQL
                     NVYNLFDTDYVASINKSGYRYHPGKPRTFLLTANMHF"
     gene            2030..2707
                     /gene="ybiX"
                     /locus_tag="OO249_000756"
     CDS             2030..2707
                     /gene="ybiX"
                     /locus_tag="OO249_000756"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5635152.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            2781..3047
                     /gene="ybiI"
                     /locus_tag="OO249_000757"
     CDS             2781..3047
                     /gene="ybiI"
                     /locus_tag="OO249_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5635153.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3312..3572
                     /locus_tag="OO249_000758"
     CDS             3312..3572
                     /locus_tag="OO249_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5635154.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLTEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3800..4885)
                     /gene="hcxB"
                     /locus_tag="OO249_000759"
     CDS             complement(3800..4885)
                     /gene="hcxB"
                     /locus_tag="OO249_000759"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5635155.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGYLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5026..5988)
                     /gene="ybiB"
                     /locus_tag="OO249_000760"
     CDS             complement(5026..5988)
                     /gene="ybiB"
                     /locus_tag="OO249_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5635156.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(6016..8166)
                     /gene="dinG"
                     /locus_tag="OO249_000761"
     CDS             complement(6016..8166)
                     /gene="dinG"
                     /locus_tag="OO249_000761"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5635157.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            8286..8768
                     /gene="ybiA"
                     /locus_tag="OO249_000762"
     CDS             8286..8768
                     /gene="ybiA"
                     /locus_tag="OO249_000762"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5635158.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPTVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(9000..10364)
                     /gene="rhlE"
                     /locus_tag="OO249_000763"
     CDS             complement(9000..10364)
                     /gene="rhlE"
                     /locus_tag="OO249_000763"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR5635159.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10593..11264
                     /gene="cecR"
                     /locus_tag="OO249_000764"
     CDS             10593..11264
                     /gene="cecR"
                     /locus_tag="OO249_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5635160.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            11267..12262
                     /gene="hlyD"
                     /locus_tag="OO249_000765"
     CDS             11267..12262
                     /gene="hlyD"
                     /locus_tag="OO249_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5635161.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            12255..13991
                     /locus_tag="OO249_000766"
     CDS             12255..13991
                     /locus_tag="OO249_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5635162.1"
                     /translation="MNDAVITLNGLEKRFPGMGKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELASEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13984..15117
                     /locus_tag="OO249_000767"
     CDS             13984..15117
                     /locus_tag="OO249_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5635163.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            15128..16234
                     /locus_tag="OO249_000768"
     CDS             15128..16234
                     /locus_tag="OO249_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5635164.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIATNYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(16196..16606)
                     /locus_tag="OO249_000769"
     CDS             complement(16196..16606)
                     /locus_tag="OO249_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5635165.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16739..17490
                     /locus_tag="OO249_000770"
                     /pseudo
     CDS             16739..17490
                     /locus_tag="OO249_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            17487..18728
                     /gene="clsB"
                     /locus_tag="OO249_000771"
     CDS             17487..18728
                     /gene="clsB"
                     /locus_tag="OO249_000771"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5635166.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18728..19684
                     /locus_tag="OO249_000772"
                     /pseudo
     CDS             18728..19684
                     /locus_tag="OO249_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(19720..20433)
                     /locus_tag="OO249_000773"
     CDS             complement(19720..20433)
                     /locus_tag="OO249_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5635167.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLVIVLWPGIISHGRNKLLELAGKCHSEKLWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(20639..21343)
                     /locus_tag="OO249_000774"
     CDS             complement(20639..21343)
                     /locus_tag="OO249_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5635168.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(21480..21932)
                     /gene="moaE"
                     /locus_tag="OO249_000775"
     CDS             complement(21480..21932)
                     /gene="moaE"
                     /locus_tag="OO249_000775"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5635169.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFAGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(21934..22179)
                     /gene="moaD"
                     /locus_tag="OO249_000776"
     CDS             complement(21934..22179)
                     /gene="moaD"
                     /locus_tag="OO249_000776"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5635170.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(22172..22657)
                     /gene="moaC"
                     /locus_tag="OO249_000777"
     CDS             complement(22172..22657)
                     /gene="moaC"
                     /locus_tag="OO249_000777"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5635171.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(22660..23172)
                     /gene="moaB"
                     /locus_tag="OO249_000778"
     CDS             complement(22660..23172)
                     /gene="moaB"
                     /locus_tag="OO249_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5635172.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(23194..24183)
                     /gene="moaA"
                     /locus_tag="OO249_000779"
     CDS             complement(23194..24183)
                     /gene="moaA"
                     /locus_tag="OO249_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5635173.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(24171..24315)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            24580..25488
                     /gene="yvcK"
                     /locus_tag="OO249_000780"
     CDS             24580..25488
                     /gene="yvcK"
                     /locus_tag="OO249_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5635174.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(25680..27701)
                     /gene="uvrB"
                     /locus_tag="OO249_000781"
     CDS             complement(25680..27701)
                     /gene="uvrB"
                     /locus_tag="OO249_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5635175.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(28280..28957)
                     /gene="bioD"
                     /locus_tag="OO249_000782"
     CDS             complement(28280..28957)
                     /gene="bioD"
                     /locus_tag="OO249_000782"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5635176.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(28950..29705)
                     /gene="bioC"
                     /locus_tag="OO249_000783"
     CDS             complement(28950..29705)
                     /gene="bioC"
                     /locus_tag="OO249_000783"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5635177.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(29692..30846)
                     /gene="bioF"
                     /locus_tag="OO249_000784"
     CDS             complement(29692..30846)
                     /gene="bioF"
                     /locus_tag="OO249_000784"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5635178.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(30843..31883)
                     /gene="bioB"
                     /locus_tag="OO249_000785"
     CDS             complement(30843..31883)
                     /gene="bioB"
                     /locus_tag="OO249_000785"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5635179.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVQGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            31970..33259
                     /gene="bioA"
                     /locus_tag="OO249_000786"
     CDS             31970..33259
                     /gene="bioA"
                     /locus_tag="OO249_000786"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5635180.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            33318..33794
                     /locus_tag="OO249_000787"
     CDS             33318..33794
                     /locus_tag="OO249_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5635181.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            33946..35229
                     /locus_tag="OO249_000788"
     CDS             33946..35229
                     /locus_tag="OO249_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5635182.1"
                     /translation="MNTFSVSRLALALAFSVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGCQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35370..37607)
                     /locus_tag="OO249_000789"
     CDS             complement(35370..37607)
                     /locus_tag="OO249_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5635183.1"
                     /translation="MFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSSHNTSGNMD
                     KLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGTINGDDHVF
                     GLSAAQRYGGIFVPPHIAIIHQYMREMMAGGGKMILGSDSHTRYGALGTMAVGEGGGE
                     LVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVG
                     PGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYY
                     DGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAHGKAKLSLL
                     DKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPSSQPVFMDL
                     AKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREGSKPANGQM
                     SAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQ
                     PLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRSNPIGLAEF
                     TLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMV
                     YAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEV
                     GDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFN
                     KNRQM"
BASE COUNT         8810 a        10184 c         9742 g         8879 t
ORIGIN      
        1 ccggcgtggg tgcgtttttg gcgggtgaga acggtaactc caccactggc gacgccattt
       61 atatgcgtgg tgccgatacc tctaacagta tttatattga tggcattcgc gatatcggca
      121 gcgtctcgcg cgacaccttc aataccgagc aggtcgaagt gattaaaggg ccgtccggca
      181 ccgactacgg gcacagcgca ccgacgggct cgatcaatat gatcagcaaa cagccgcgca
      241 atgattccgg cattgacgcc tccgccagta ttggcagcgc ctggttccgc cgcggcacgc
      301 tggacgtcaa tcaggtcatt ggtgatacca ccgcggtgcg cctgaatgta atgggcgaaa
      361 aaacgcacga tgccggacgc gacaaagtca aaaatgagcg ttacggcgtc gccccttctg
      421 tcgcttttgg ccttggtaca gcgaatcgtt tgtatcttaa ttatctgcat gtcacccagc
      481 acaacacgcc agacggcggc attccgacca tcggtttgcc gggctattct gccccatctg
      541 cgggaacggc ggccctgaat cattccggaa aagttgatac tcataacttt tacggcacgg
      601 attccgatta cgacgattcg accaccgaca ccgccaccat gcgttttgag cacgacatca
      661 acgataacac caccattcgc aatactaccc gttggtcgcg cgtaaagcag gattacctga
      721 tgacggcgat tatgggcggg gcgtcgaata ttactcagcc caccagcgat gtgaatagct
      781 ggacctggtc acgcacggcg aataccaaag atgtgagtaa taaaattctc accaaccaga
      841 ccaacctgac ctcgacgttc tataccggtt ctatcggtca tgatgtcagt accggcgtgg
      901 aatttacccg tgaaacgcag actaactacg gcgttaatcc ggtgacgtta cccgcggtaa
      961 atatttatca tcctgacagc agcattcatc ccggcggcct gacgcgcaac ggcgcaaacg
     1021 ccaatggtca gacggatacc ttcgcaattt acgcctttga tacgctgcaa atcacccgtg
     1081 attttgagct gaacggcggg atccgtctgg ataattatca tactgaatat gacagtgcca
     1141 ccgcctgcgg cggcagcgga cgcggtgcca tcacctgccc agctggtgtg gcaaaaggtt
     1201 ctccggtcac caccgtcgac accgccaagt cgggcaatct ggtgaactgg aaagccgggg
     1261 cgctgtatca cctgacggaa aacggtaatg tctatattaa ctatgccgtt tcccagcagc
     1321 ctccgggcgg caacaacttc gcccttgcgc agtctggcag cggtaacagt gccaaccgca
     1381 ccgattttaa accgcaaaaa gccaacacca gcgagattgg caccaaatgg caggttctgg
     1441 ataaacgcct gttgctcacc gccgcactgt tccgtactga tatcgaaaat gaagttgagc
     1501 aaaatgatga cggaacttac tcgcaatacg gtaagaaacg tgtcgaaggc tatgagatat
     1561 ccgtggccgg gaatatcact cccgcgtggc aggtgattgg cggctatacc cagcaaaaag
     1621 caaccatcaa aaacggcaaa gatgttgccc aggatggttc ctcatcgctg ccgtataccc
     1681 cggagcacgc cttcacctta tggagccagt atcaggcaac cgatgatatc tctgttggcg
     1741 cgggcgcacg ctatatcggc agtatgcata aaggttcaga cggcgcggtg ggaacgccag
     1801 cgtttaccga aggttactgg gtcgccgatg ccaaactggg gtatcgggtt aatcgcaatc
     1861 tcgacttcca gctaaacgtt tacaacctgt ttgataccga ttacgtcgcc tcaatcaaca
     1921 agagcggcta ccgttatcac ccgggcaagc caagaacctt cttgctcaca gccaatatgc
     1981 atttctgatt cagatgtggg gcgcaggccc cactttttgg agaaattgta tgatgtacca
     2041 cattcccggc gtgttatcgc cacaggacgt cgctcgtttt cgcgaacaac tggaacaagc
     2101 cgaatgggtg gatggacgcg tcaccaccgg cgcacaaggt gcgcaagtta agaacaatca
     2161 acaggtagac acccgcagca cgttatacgc cgccctgcaa aatgaggtgc tgaacgcggt
     2221 taaccaacat gctttattct ttgccgcggc cttgccgcgt accctttcca cgccgctgtt
     2281 taatcgctat cagaacaatg aaacctatgg ttttcatgtg gatggcgcag tacgcagcca
     2341 tccacaaaac ggctggatgc gcactgacct ttctgccacg ctgtttttaa gcgatccaca
     2401 aagctacgac ggcggcgaac tggtcgttaa tgacaccttc ggacaacatc gggtaaaact
     2461 cccggcaggt gatctcgtgt tgtatccctc cagcagcctg cattgcgtga cacccgtaac
     2521 ccgcggcgta cgagtggcat catttatgtg gatccagtcg atgatccgcg atgataaaaa
     2581 gcgcgccatg ctgtttgaac tagacaaaaa tattcagtcg ctgaaaagcc gctatggtga
     2641 aaatgaagag atcctgtcgc tgctcaatct ttatcataat ctgctgcggg aatggtcgga
     2701 gatctgacgc ttgaactgcc caggttcgtc cctatattag ctacacttaa ctgtacaagc
     2761 attgatatgg ggaggacgat atggcatccg gttgggctaa cgatgacgcc gtcaacgaac
     2821 agatcaacag tacaattgaa gatgcgattg cccgcgctcg gggtgaaatt ccgcgtggcg
     2881 aaagcctgta tgaatgtgaa gagtgcggtg cccccatccc gcaagcccgt cgggaagcca
     2941 ttcctggcgt gcgcttatgt attcattgtc agcaggagaa agatttacaa aaaccagcgt
     3001 atacaggata taatcgcaga ggttcgaaag acagccagtt acgttaacta caactggcga
     3061 gatgcatagc gagtataatt tgtattttgc gtggcattcc gtgcctttaa cgctgctgtg
     3121 actgccacgc ggcataaaac aagtccataa gtacaacaaa taaatggttt atcagtatgt
     3181 tagatgtcaa tcaatttatt tgaacaaggc ggtcaattct cttcgatttt atctctcgaa
     3241 aaaaaacgtg atactcatca catcgacgaa acaacgtcac ttatacaaaa atcacctgcg
     3301 agagattaat tatgaaaact atcaatactg ttgttgctgc tatggctctt tcaactctat
     3361 catttggcgt gttcgcggcg gaaccggtaa cggcatccca ggcacagaac atgaataaaa
     3421 tcggcgtggt ttctgccgat ggcgcatcca ccctcgatgc cctggaagcg aaactgactg
     3481 agaaagccgc ggcagccggt gccagtggat acagcatcac ttctgccacc aacaacaata
     3541 aattaagcgg tactgcggta atttataagt aattcgtagg acggataagg cgtttacgcc
     3601 gcatccggca acagatgcct gatgcgacgc taccgcgtct tatcaggcct acaaaatccg
     3661 aaccgttgga cggatcaggc ggttacgcta cgtccagcac aagtatttcc ctgtctgttt
     3721 gccgacagac gcatatgctc taaccctcat tgatcctata ttacccttgt ttgcccgtcc
     3781 gttactagac gggctttttt tagctggcta actgctgaca gaaagcctgc aacgtctctt
     3841 ccgacatacc aatctgccgc gccgcatcac aaatggcctg ccagcttccc gcatccaatg
     3901 gaatcccctg ctcctgccgt tcgcgacgcg tgttcacttc ccactcgccc ggtagcaaaa
     3961 tcggcttatc gtcatcatgc ggcgaggctt tcacccactc ggcaaaggct tcagtctgcg
     4021 cgctacaatc cggcgcgccg aacagttccg ggttgatgat gatagtggtc atgcagttaa
     4081 gaatggcatc gggactggtt tgtaacgttt cctgatgcgt cgttttaccg ccagaaagcg
     4141 ccccgccaag aatttcacac atcgctgcaa gggcgtagcc tttatgttcg gcaaaggtca
     4201 acagcgaccc caacggcgac tcctgcatta ccgccggatt ggtcgtcggc acgccgttaa
     4261 cgtcaatcag gcaacctggc ggcacgggga cgcctttatg ccaggcgacg cgagttttac
     4321 caaatgcgat ggcgctggtg gcgtaatcaa gcaacagcgg gaaattatct ttacgaggga
     4381 aaaccacaca gaacggattg gtgccaaagc ggctgtcgcg accgtggaac ggtgcgacca
     4441 ttggaatacc gaccacgcta acaaagtgga tagagacaaa ccccgccgct gcacactgct
     4501 ccgcccagta accgatacgg ccgatatgat gcgagttatg tagcgccacg gcagcaatac
     4561 cgtgctgatg cgctttctca atccccagcg ccatcgcttc gtgtgccgcg acctgaccga
     4621 atgcgcgatc gccatcgagc gtgactgccg cccccgcctc tttaacgatt ttggcatgat
     4681 ggttaatttg cagatacccc tgactccagg aacgcacata gcttgggatc atgccaatac
     4741 catgagaatc atgccctgcc aggtttgccg cgattaaatg atcggccact aatttcgctt
     4801 cttgttcctc gctacccatc tgacgaaata cagcctgaat aaaactgtgc agcgtctgag
     4861 catcaaagcg atgaccactt tccatggtta actcctgttt atgttatgtg tttgttgttt
     4921 ttttatgctg cacgccgggc gcgggaaggt caataaagat gcaggaattt catattgcgg
     4981 tggggtaaaa gccggatgac atggctcatc cggctgagaa aagaattaaa atgcctgatt
     5041 aacgcgcgcc aggccgtcgc tgatagttgc cgcttcaccc gtagcctcca ggcagcaagc
     5101 catctggatt ttcagcgatt cgggaatcgg ttcgctgcca gcaaggcaac gctcaatcca
     5161 ctgcgccgtg gtttctgggt cttttgcttg tggcagtaac tcgctaccag cagtgtcctg
     5221 tttttcatac agcacccgca tcccttcacg gtcaatgaga ttgatctgcg ggcagcgctg
     5281 cggattagca tacacttcgc cttcagtacc atgcattagt agcgcccgcc cgccgatatc
     5341 gctaaagaac ttcgcgactc gcccgatgta ttccggatgc gaaacgctgg aaagacgcag
     5401 cgcctcacct tcggcaaatg gcgtcgccag tttcgccagg gtatgtgcac tgttacgcac
     5461 gcccatccgc cagcgcatcg ccagttgttt ttccagcggc gggcaaaacg cgccgacagg
     5521 cataaacacc ggttgatgtt cgtcgagctt cgcctgcgcc tgcccgccgt gcaacgttgg
     5581 tgtaataccc atcagttcga aaatggtttc tgtcagcacg cgagttggat cttcgctaac
     5641 cccgtgaacc accacaggaa aaccgagttt gtgcaggaga atagccaaca acggcgtcag
     5701 gttggcctgt ttacgcgcgc cgttgtaact ggggatgaca atcggcatcg gcttgcctgc
     5761 aggcggcgtc agcttgatgg tgtgattttg catggcttcg taaaagccga gcatctctgc
     5821 ttccccttcc cctttgatac gtagcgcaat caatacgccg cccaactcaa ggtcagggac
     5881 ttcaccattg agcatatggg catacagacc gcgcgcagta tcccggtcta aatcgcgcgc
     5941 gtggtttttc ccgcgcccga tctctttaat gattttgcga tagtccattt acgactcctt
     6001 acctgactca catcattaac gccgccgacg gcgtggggat ttcgtttttt ctttcttttt
     6061 aactatacct tcagggactt ccggttgctc tatcggaaat accggtaatg catccagtag
     6121 tcgcttgcca taatttttgg tcagcaagcg tttgtcgtag ataaccactt cgccccagca
     6181 accgtggctt cgaatcagtc gcccaacctg ctgaatcagg ttaaacgagg cgctcggcag
     6241 gctttgcacc tcaaacggat agcggttgag gcttttcagc cattcccctt cggtgatcac
     6301 caccgggctg tcgatgggcg gaaaagcgat tttgtggata tgcacctggc tgagcagatc
     6361 acctttcaaa tcaagccctt cggcaaatga ctgtaagccc accagcacac tacgctcacc
     6421 gttggcaacg cgtttgcggt gcagttcaac taagcgataa cgcggctgat cgccctgcac
     6481 cagcagcatc aggcgtaaat ccgtcacata gtcgagaaag cgctgcatcg cccgtccgct
     6541 ggcaaacagt accaacatac cgagatgttt tttgctctcc acctgctcac ggaaaaaggc
     6601 cgccatttcg gcaatatgct gctcttcgtt gtcgatggaa ggctcaaagc gcatccgggg
     6661 aataacaatt ttgccctgtt cgcagtggtt aaagggggaa tccagcgcca caaaacggtc
     6721 gcccgctttc tctttcagcc cgctcatctc ctgcaaacgc gaaaaactgt tcagcgaacg
     6781 caaggttgcg gaggtgacaa taatgtgcgg aatactgcgc cacagcagcc tttccagctg
     6841 atcgctgaca cgtattccca cgcagtgaaa ccagagatgc agctgccctt cgcgctcttc
     6901 ccgcgtcgcc catttggtca ccggcgcacc ggaagattgc gccagagacg ccagccgcca
     6961 gagtttgctt tgcgcctcga acatccccaa cgcgcggttc atctgcaaaa tcaaccgatg
     7021 cagacgcaca atatcgtggc tgccggtttt ctcacttaaa tcgttaagaa ataactccgc
     7081 cagaccacgc agcatctcgg tgagttttgc cagccgctgg cagatctcca gcacttcatc
     7141 aggcagttcg cccatcgcaa aacggtgctc tgcctcctgc ccggcaggca tgtagagatt
     7201 gagaatgttg tttaacgagg cgataagctc atacaactct tcacaatgcg cattcaaacg
     7261 ttcagggatc gccagcggtg ggatggtctt cgggcgaaac tgctccatgc aggtagcgac
     7321 cagtttcgtg aacaagtcca gctgtagtcg ataccagggc gcggtgattt cagcgctcat
     7381 ctccagcgca tcccgggcca catctggcag gtgatgacct tcgtccagca ccagcagcag
     7441 atttttcggg tcaggcagta cggcttcgct ttccatcgcc gccatcacca gcgcatggtt
     7501 tgccaccacc acttccgctt cctgaatctc ccgacgagca acaaaaaacg ggcattcacg
     7561 atagtagtaa cagttacggt taaggcagct ggctttgtcg gtactcaaac gacgccagag
     7621 atcatcatct atagcgatat ccgtatgatc gcgcagacca tcccatttat aagtgtcgag
     7681 atcgcccttc agcttcgcac aacgtttttg ctcttcctga ttatttggcg tcagttcgtc
     7741 gtcaagaaac gccagcagat cctgttgcgt gggttcagtg ctggcgagcg ccgtcagatt
     7801 acgcggacaa acgtagcgcc cacgcccgaa agcggcagtg aatttaagat cgggaatgat
     7861 ctttttcagc agcggtaaat ctttgctgta aatctgatcc tgcaacgcca cgttggcggt
     7921 actcaccacc agcgtttttt gctcttcgcg ggcaatggcg atgccgggga tcaaatagga
     7981 gagcgttttc ccaacgccgg tgggggcttc aatcgccaga tgccgccctt cttctccggc
     8041 cagcgttttg gcgacgtccg caatcatctg ccgctgcggc gcacggggaa taaagtcggg
     8101 gatctgttcc tgaagcgcct tataccaggc ggcaatttgc gctttaagcg cggcggttaa
     8161 tgccatgaga aaacctgaaa tactgtataa acagccaata ttgtggcatt tttcggtttt
     8221 caggggtaat ggtttttctg tttctggaaa gcggctcgca ataaccacca acataatgcg
     8281 gtttaatgcc cgttcgagca caaaggatcc aacacgtcat gcaagacacc atcataaatt
     8341 tctacagcac cagcgatgac tacggtgatt tttctaactt tgccgcctgg cccatcaagg
     8401 ttgacgggaa aacctggccc acctccgaac actattttca ggcacaaaaa ttccttgatg
     8461 aaaaataccg cgaagagatc cgtcgggttt cttcccccac ggtcgccgct cgcatggggc
     8521 gtaatcgttc caagccactt cgtaaaaact gggagtcggt taaagaacag gtgatgcgta
     8581 aagcacttcg tgctaaattc gaacagcatg cagaactgcg tgcgctcttg ctggcaaccg
     8641 cgcccgcaaa actggttgag catacggaaa acgatgctta ctggggagac ggtggtaatg
     8701 gtaagggcaa gaatcgactg ggctaccttt taatggagtt gcgcgaacaa ttggctatag
     8761 agaagtaacg tctcggtggc ttatgggcgg taaaacagat aaattttttc ccgtgttctc
     8821 aaattgctga caagtgcttg ttgtgaaatg ccggatgcgg cgtaaacgcc ttatccggcc
     8881 tacaaaagca tgcaaattca atatattgca gagttaacgt aggcctgata agcgtagcgc
     8941 atcaggcagt tttgcgtttg ttcatcagcc tgatgccggg catagcccgg cataaaagat
     9001 tactgcgcag cggcaggttt acgcggacgg cggcgacgtt gttgttcgcc tgccggtttg
     9061 gcatcgccga ggcggcgaga cggtttttct gcaggtttcg cgcttgcaga ttttgcgcca
     9121 ccttccccac ggcgtggttg ctgttgaccg cgaccaccac cttgcccacg accgccgccg
     9181 ccacgttgct ggcgaccgtt ctggatcggt tcggctttga ttgacgggtc cggctcatag
     9241 cccggaatcg caatgcgcgg gatctctttt ttcagcagtt tttcgatatc acgcagcagt
     9301 ttgtgttcat caacacacac cagcgacaac gcttcaccgg tagcagccgc acgaccggta
     9361 cgcccgatac ggtggacata atcttccggt acgtttggca gttcatagtt gacgacgtgc
     9421 ggcaactctt cgatatccag accgcgcgca gcgatatcgg tcgccaccag tacgcgaatg
     9481 tcactcgatt taaaatcagc cagcgcacgg gtacgcgcac cttgcgattt attcccgtgg
     9541 atcgccgcac tacggatgcc atctttattg agctgctcag ccagatggtt agcgccgtgt
     9601 ttggtacggg taaacaccag cacctgctgc cagttccctt taccaatcat gtgcgacagc
     9661 aattcgcgtt tgcgtttctt atcgacaaag tgaacgtgct gggtcacctg atcagacgcg
     9721 gtattgcggc gtgccacttc gatttccagc gggttgtgca acagtttttc cgccagggct
     9781 ttaatatcgt cagagaaggt cgcagagaac agcaggttct ggcgtttcgc tggcagtttt
     9841 gttaacacgc gacggatatc gtggataaag cccatgtcga gcatgcggtc agcttcatcg
     9901 aggacgagga tttcaacctg atccagcttc actgcatttt gatgttccag gtccagcaaa
     9961 cgtcccggtg ttgccaccag aacatcaacg ccgccgcgca gcttcatcat ttgcgggtta
    10021 atgctgacac cgccaaacac caccagcgaa cgaatgttca aatatttgct gtaatcacgg
    10081 acgttttcgc caatctgcgc agccagttca cgggtcgggg taagaatgag cgcacgtacc
    10141 ggacgacgcc ctttggcgtg cggctggcga gtgatcaggt gttgcaacag cggcagcgta
    10201 aagcccgctg ttttaccggt accggtctgg gcgctagcca tcaggtcgcg gccttccagc
    10261 accgcaggga tcgcctgctg ctgaataggg gtgggttcac ggtaaccctg ctcggcaacg
    10321 gcgcgcagga tatcagggct taaacccaaa gaatcgaaag acataactac tccgaaccgc
    10381 cccgaccgtt accggtgtag ttttcaggga gatactgaaa aggaaaatga caaaaaccac
    10441 aatgtcatga gggagcggag tgtagcagtt tttgtgacgc agcgcataaa ttatccctgt
    10501 gactggcgtt gctaaaattc ttagccatac tggacaactc ccctatggag tcataatctt
    10561 aatcaatcat ttgattaaga atggactgtg cgatgaataa tcctgccatg acaatcaagg
    10621 gtgaacaggc gaaaaaacag ctgattgctg ccgcactggc gcagtttggt gaatatggaa
    10681 tgaacaccac cactcgcgag atagccgccc aggccgggca gaatatcgct gccatcacct
    10741 actacttcgg ttcgaaagaa gatttgtacc tcgcctgcgc ccagtggatt gccgatttta
    10801 ttggcgagca gttccgtccg catgccgagg aagccgaacg cttgttcgcg caaccacagc
    10861 ctgatcgggc tgccatccgt gaactgatcc ttcgcgcctg caggaacatg attaagctgc
    10921 tcacccagga tgacaccgtc aacctcagca agtttatctc ccgtgagcag ctctctccca
    10981 cggcagccta ccacctggtg cacgaacagg tgattagtcc gctacacagc cacctgacac
    11041 gcctgattgc cgcctggacc ggctgcgacg ccaatgacac ccgcatgatc cttcataccc
    11101 atgcgttgat tggtgagatt ctggcgttcc gtcttggcaa agaaacaatt ctgttacgta
    11161 ccggctggac cgcgttcgat gaagaaaaga ccgaactgat caaccagacg gtgacctgtc
    11221 atatcgacct gattttgcaa ggattatcgc aaaggagttt gtagtgatga aaaaacctgt
    11281 cgtgatcgga ctggcggtag tggtacttgc cgccgtggtt gccggaggct actggtggta
    11341 tcaaagccgc caggataacg gcctgaagct gtatggcaac gtggatattc gtacggtgaa
    11401 tcttagtttc cgtgttgggg ggcgcgttga atcgctggcg gtggacgaag gtgatgcgat
    11461 caaagcgggc caggtgctgg gcgaactgga tcacaagccg tatgagattg ccctgatgca
    11521 ggtgaaagcg ggcgtctcgg tggcacaggc gcagtatgac ctgatgcttg ccgggtatcg
    11581 cgatgaagaa atcgctcagg ccgccgcagc ggtgaaacag gcgcaagccg cctatgacta
    11641 tgcgcagaac ttctataacc gccagcaagg actgtggaaa agccgcacca tttcggcaaa
    11701 tgacctggaa aatgcccgct cctcgcgcga ccaggcacag gcaacgctga aatcagcaca
    11761 ggataaattg cgtcagtacc gttccggtaa ccgtgaacag gacatcgctc aggcgaaagc
    11821 cagcctcgaa caggcgcagg cgcaactggc gcaggcggag ctgaatttac aggactcaac
    11881 gttgatagca ccgtctgatg gcacactgtt aacgcgcgcg gtggagccag gcacggttct
    11941 caatgaaggt ggcacggtgt ttaccgtttc actaacgcgt ccggtgtggg tgcgcgctta
    12001 tgttgatgaa cggaatcttg accaggccca gccggggcgc aaagtgctgc tttataccga
    12061 tggtcgcccg gacaagccgt atcacgggca gattggtttc gtttcgccga ctgctgaatt
    12121 taccccgaaa accgtcgaaa caccggatct gcgtaccgac ctcgtctatc gcctgcgtat
    12181 tgtggtgacc gacgccgatg atgcgttacg ccagggaatg ccggtgacgg tgcaattcgg
    12241 taacgaggca ggacatgaat gatgccgtta tcacgctgaa cggcctggaa aaacgctttc
    12301 cgggcatggg caagcccgcc gtcgcgccgc tcgattgtac cattcacgcc ggttatgtga
    12361 cggggctggt ggggccggat ggtgcaggta aaaccacgct aatgcggatg ctggcgggat
    12421 tactgaaacc cgacagcggc aatgccacga tgattggctt tgatccaatc aaaaacgacg
    12481 gcgcgctgca cgccgtgctc ggctatatgc cgcagaaatt tggtctgtat gaagatctca
    12541 cggtgatgga gaacctcaat ctgtacgcgg atttgcgcag cgtcaccggc gaggcacgta
    12601 agcaaacttt tgctcgcctg ctggagttta cgtctcttgg gccgtttacc ggacgcctgg
    12661 cgggcaagct ctccggtggg atgaaacaaa aactcggtct ggcctgtacc ctggtgggcg
    12721 aaccgaaagt gttgctgctc gatgaacccg gcgtcggcgt tgaccctatc tcacggcgcg
    12781 aactgtggca gatggtgcat gagctggcaa gcgaagggat gttaatcctc tggagtacct
    12841 cgtatctcga cgaagccgag cagtgccgtg acgtgttact gatgaatgaa ggcgagttgc
    12901 tgtatcaggg ggaaccaaaa gccctgacac aaaccatggc cggacgcagc tttctgatga
    12961 ccagtccaca cgagggcaac cgcaaactgt tgcaacgcgc cttgaaactg ccacaggtca
    13021 gcgacggcat gattcagggg aaatcggtac gtctgatcct caaaaaagag gccacaccag
    13081 acgatattcg ccatgccgac gggatgccgg aaatcaacat caacgaaact acgccgcgtt
    13141 ttgaagatgc atttattgat ttgctgggtg gtgccggaac ctcagaatcg ccgctgggtg
    13201 caatattgca tacggtagaa ggcacacccg gcgagacggt gatcgaagcg aaagaactga
    13261 ccaagaaatt tggtgatttt gccgccaccg atcacgtcaa ctttgccgtt aaacgtgggg
    13321 agatttttgg tttgctgggg ccaaacggcg cgggtaaatc gaccaccttt aagatgatgt
    13381 gcggtttgct ggtgccgact tccggccagg cgctggtgct ggggatggat ctgaaagaga
    13441 gttccggtaa agcgcgccag catctcggct atatggcgca aaaattttcg ctctacggca
    13501 acctgacggt cgaacagaat ttacgctttt tctctggtgt gtatggctta cgcggtcggg
    13561 cgcagaacga aaaaatctcc cgtatgagcg aggcatttgg cctgaaaagt atcgcctccc
    13621 acgcgaccga tgaactgcca ttaggtttta aacagcggct ggcgctggcc tgttcgctga
    13681 tgcatgaacc ggacattctg tttctcgacg aaccaacgtc cggcgttgat cccctcaccc
    13741 gccgcgaatt ttggctgcat atcaacagca tggtagagaa aggcgtcacg gtgatggtca
    13801 ccactcactt tatggatgaa gcagaatatt gcgaccgcat cggcctggtg taccgtggga
    13861 aattaattgc cagcggcacg ccggatgatt tgaaagcgca gtcggccaac gacgagcaac
    13921 ccgatcccac catggagcaa gcctttattc agttgatcca cgactgggat aaggagcata
    13981 gcaatgagta acccgatcct gtcctggcgt cgcgtacggg cgctgtgcgt taaagagacg
    14041 cggcagatcg ttcgcgatcc gagtagctgg ctgattgcgg tagtgatccc gctgctactg
    14101 ctgtttattt ttggttacgg cattaacctc gactccagca agctgcgggt cgggatttta
    14161 ctggaacagc gtagcgaagc ggcgctggat ttcacccaca ccatgaccgg ttcgccctac
    14221 atcgacgcca ccatcagcga taaccgtcag gaactgatcg ccaaaatgca ggcggggaaa
    14281 attcgcggtc tggtggttat tccggtggat tttgcggaac agatggagcg cgccaacgcc
    14341 accgcaccga ttcaggtgat caccgacggc agtgagccga ataccgctaa ctttgtacag
    14401 gggtatgtcg aagggatctg gcagatctgg caaatgcagc gagcggagga caacgggcag
    14461 acttttgaac cgcttattga tgtacaaacc cgctactggt ttaacccggc ggcgattagc
    14521 cagcacttca ttatccccgg tgcggtgacc attatcatga cggtcatcgg cgcgattctc
    14581 acctcgctgg tggtggcgcg agaatgggaa cgcggcacca tggaggctct gctctctacg
    14641 gagattaccc gcacggaact gctgctgtgt aagctgatcc cttattactt tctcgggatg
    14701 ctggcgatgt tgctgtgtat gctggtgtca gtgtttattc tcggcgtgcc gtatcgcggg
    14761 tcgctgctga ttctgttttt tatctccagc ctgtttttac tcagtaccct ggggatgggg
    14821 ctgctgattt ccacgattac ccgcaaccag ttcaatgccg ctcaggtcgc cctgaacgcc
    14881 gcttttctgc cgtcgattat gctttccggc tttatttttc agatcgacag tatgcccgcg
    14941 gtgatccgcg cggtgacgta cattattccc gctcgttatt tcgtcagcac cctgcaaagc
    15001 ctgttcctcg ccgggaatat tccagtggtg ctggtggtaa acgtgctgtt tttgatcgct
    15061 tcggcggtga tgtttatcgg cctgacgtgg ctgaaaacca aacgtcggct ggattaggga
    15121 gaagagcatg tttcatcgct tatggacgtt aatccgcaaa gagttgcagt cgctgctgcg
    15181 cgaaccgcaa acccgcgcga ttctgatttt acctgtgctt attcaggtga ttctgttccc
    15241 gttcgccgcc acgctggaag tgactaacgc caccatcgcc atctacgatg aagataacgg
    15301 cgagcattcg gtggagctga cccaacgttt tgcccgcgcc agcgccttta ctcatgtgct
    15361 gctgctgaaa agcccacagg agatccgccc aaccatcgac acacaaaagg cgttactcct
    15421 ggtgcgtttc ccggctgact tctcgcgcaa actggatacc ttccagaccg cgccgttgca
    15481 gttgatcctc gacgggcgta actccaacag tgcgcaaatt gccaccaact acctgcaaca
    15541 gatcgtcaaa aattatcagc aggaactgct ggaaggaaaa ccgaaaccta acaacagcga
    15601 gctggtggta cgcaactggt ataacccgaa tctcgactac aaatggtttg tggtgccgtc
    15661 actgatcgcc atgatcacca ctatcggcgt aatgatcgtc acttcacttt ccgtcgcccg
    15721 cgaacgtgaa caaggtacgc tcgatcagct actggtttcg ccgctcacca cctggcagat
    15781 cttcatcggc aaagccgtac cggcgttaat tgtcgccacc ttccaggcca ccattgtgct
    15841 ggcgattggt atctgggcgt atcaaatccc cttcgccgga tcgctggcgc tgttctactt
    15901 tacgatggtg atttacggtt tatcgctggt gggattcggt ctgttgattt catcactctg
    15961 ctcgacacaa cagcaggcgt ttatcggcgt gtttgtcttt atgatgcccg ccattctcct
    16021 ttccggttac gtttcgccgg tggaaaacat gccagtatgg ctgcaaaacc tgacgtggat
    16081 taaccctatt cgccacttta cggacattac caagcagatt tatttgaagg atgcgagtct
    16141 ggatattgtg tggaatagtt tgtggccgct actggtgata acggccacga cagggtcagc
    16201 ggcgtacgcg atgtttagac gtaaggtgat gtaacttctt atctttcgcc agcaaagaca
    16261 ctaccgccgg gccggcaagg attgccagcc ctgcaattgc cagcaaaaag ttgttttcca
    16321 gtacgcggga caacagccac agcacaatca gcgctgcgcc aaaataccag gtcgtggtca
    16381 gctcttccaa cacgtctgcc acctcacgcc agcgttgctc ggggtggcgc tgaaacacca
    16441 acatcatcac taccgtgaca caatacagtg cgcataatcc cacgccgacg tgaaccagag
    16501 tcttactcat ccagcccacc accagcagcg ccactaccag taaatgcaac ataatctgcc
    16561 agcaacttag acccgttgcg acacgaacac gttgttgcca cttcatggct tctctcctga
    16621 agaatttatc tctacttact cagagtaccg aactttacgg aaaattccgt aagaccgcac
    16681 ctttttttga cgccgactac actatttcaa tgagataaga ggcaaagaca ggaaaattat
    16741 gcccgatcaa acacaacaat tttcgttcaa ggtgctcacc atcaacattc aaaaaggttt
    16801 taccgcgttt aaccgacgct tcattttgcc ggaacttcgc gacgcggtgc gtaccgtcag
    16861 cgccgatatt gtttgcctgc aggaagtgat gggcgcgcac gaagttcatc cgctgcatgt
    16921 ggaaaactgg cccgatacct cgcactacga gtttctcgcc gacactatgt ggagcgattt
    16981 tgcctacggt cgcaatgccg tatacccgga agggcatcac ggcaacgccg tactgtcgcg
    17041 ttatcccatt gaacattatg agaatcgcga tgtttcggtc gatggtgcgg aaaagcgcgg
    17101 cgtgctctac tgccgcattg tgccgccgat gaccggaaaa gcgattcatg tgatgtgcgt
    17161 acatctgggc ctgcgtgagg cgcaccgtca ggcgcagctt gcgatggggt gaatgagcta
    17221 ccggacggcg aaccggtatt ggtggcgggt gatttcaatg actggcggca aaaagctaat
    17281 catccgttaa aagtgcaggc cggactggat gagattttta cccgcgccca cggacgcccg
    17341 gcgcgcacgt ttccggtgca atttcctcta ctacgactgg acaggatcta cgtcaaaaat
    17401 gccagcgcca gcgcgccaac cgcgttgccg ctgcggacat ggcgacacct ttctgatcat
    17461 gcccctttaa gtgcggagat tcatttatga aatgtagctg gcgcgaaggc aataagatcc
    17521 agttgctgga aaacggcgag caatattatc ccgcggtgtt taaggcgatt ggcgaggcac
    17581 aagaacgcat cattcttgaa acgtttatct ggtttgagga taacgtcggc aaacagctgc
    17641 atgcggcact actggcagca gcgcaacgcg gggttaaagc ggaagtcttg ctggatggct
    17701 acggttcgcc ggatctcagc gatgagtttg tcaataaact gacggcagct ggcgtagtgt
    17761 tccgctacta cgatccccgc cctcgccttt ttggtatgcg caccaatgtg tttcgccgga
    17821 tgcatcgcaa aattgtggtg atcgacgcgc gtatcgcctt tattggcggg ctgaattact
    17881 ccgccgagca tatgtccagc tacggtccag aggctaaaca ggattacgcg gtacgccttg
    17941 aagggccgat tgtcgaagat attctccagt ttgagctgga aaacctgcct ggacagagcg
    18001 cggcacggcg ctggtggcga cgtcatcaca aagcggaaga gaaccgccag ccgggagaag
    18061 cgcaggtatt gctggtctgg cgcgataacg aagaacatcg cgatgatatt gaacgccatt
    18121 atctgaaaat gctcactcag gcgcggcggg aagtgattat cgccaacgcc tacttcttcc
    18181 ccggctatcg atttttacac gccttgcgta aagcggcacg gcgcggggtg cggatcaaac
    18241 tgatcattca gggcgaaccg gatatgccga ttgtcagagt cggtgcgcgc ttgctgtata
    18301 actatctggt taaaggcggc gttcaggttt ttgagtaccg ccgccgcccg ctccacggca
    18361 aagtggcatt gatggacgat cactgggcga cagtagggtc cagtaatctc gatccgctca
    18421 gtttgtcact gaatctcgaa gcaaatgtca tcatccacga tcgtcatttt aaccagacgc
    18481 tgcgcgataa tctgaacggc attattgccg cagattgtca gcaggtggat gaaaccatgc
    18541 tgcccaaacg cacctggtgg aacctgacca aaagcgtgct ggcgttccac tttttacgcc
    18601 acttcccggc gctggttggc tggcatccgg cacacacacc acgtctgacg caggttgatc
    18661 cccccgcaca accgacaatg gaaacgcagg atcgggtaga aactgaaaac acgggggtaa
    18721 aaccctgatg agtaaatcac acccgcgcta gcgcttagca aagaagatcc tcacctggct
    18781 gttttttatc gcggtgattg tgttgctggt ggtctacgcc aaaaaagtgg actgggaaga
    18841 ggtctggaag gtcatccgcg actacaatcg cgttgcgctg cttagtgcgg tcgggctggt
    18901 ggtcgtcagc tatctgattt acggctgcta tgacctgctc gcccgctttt actgcggtca
    18961 caaactggcg aagcgccagg tgatgctggt gtcgtttatc tgctacgcct tcaacctgac
    19021 gctcagtacc tgggtcggcg gcattggtat gcgctatcgt ttgtactccc gactagggtt
    19081 accgggcagc actattacgc ggattttctc gctcagtatt accaccaact ggctgggcta
    19141 tattttactg gcagggatta tctttaccgc aggcgtggtg gtgttgccgg atcactggta
    19201 tgtcgctcaa actacgctgc gtattctcgg cattggctta ctgatgatta tcgcggttta
    19261 tttgtggttt tgcgctttcg cgaagcaccg ccatatgacc atcaaagggc aaaagctggt
    19321 gctgccttca tggaaattcg ccctcactca gatgctgatt tccagcgtta actggatggt
    19381 aatgggggcg attatctggc tgttacttgg tcaaagcgtg aactatttct ttgtactggg
    19441 cgtgttactg gttagtagta ttgctggcgt catcgtgcat attccggcgg ggatcggtgt
    19501 gctggaagcg gtgtttatcg cactactggc tggggagcat acctcgaaag ggagcattat
    19561 cgccgcgctg ctcgcttatc gcgtcctgta ttactttatc ccgctgctgc tggcgctgat
    19621 ctgttacctg ctactggaaa gccaggcgaa gaagctacgg gcgaaaaatg aagcggcgat
    19681 gtgaaattta gttaagttct ttagtatctg cattcacgac taatgaaaaa aacgcgtatg
    19741 cctttgccag acaagcgtta tagctatcgc ggcaagtatc ccaaagaagc cgataaaata
    19801 gtaatagagt gtcaatgcac ctgtaaaagc gcaacgaacc ggactccaca acttttcact
    19861 atggcatttt cctgccagtt caaggagctt atttcgtcca tgcgagataa tgcctggcca
    19921 taacacaatc actaagcaac ttattaccca aatgggccgt tcactcatta atgccgcatt
    19981 cacgatgatt accagggcca accctgtcaa cgtcatcata atgataaaac ggtgagaacc
    20041 aggatcaaca ttaaataata aaccggccag catactgatt aaaaacatcg cgcccgcagt
    20101 gctgaacaca atgataatgg catatacagg aactaaactt gcaacgacaa tcgccaccaa
    20161 agagagtaaa aacagatata ttacgcctgc gatgacggca tttatcttgt tgatatcctt
    20221 acttaagcca tacatacaaa ctaataataa ccctatggcg acatacattg ttggtgaatc
    20281 atatttggtt gccgtaattt cgagatactt agctccccag gccagtcctg cggttgccac
    20341 caaacctaaa gcggtaatga gccatatacc gtttaatatc ctggtctcgt gctgaaaatc
    20401 taatttattg ctgttttgtg agtatgattc cattctttta gttgtcctta gaaaaaatat
    20461 taatattcac catcaataaa tttaaaccca cgcatacaaa atacttttgt atttatggat
    20521 acggaaaacg cttccgctcg gccataaggc gtattttata cctggtactt gttaagtgtc
    20581 gtttttagta agaaaagaaa tcccgacaca tggccgggat attgatggtg aaaaagaatt
    20641 aacggcggtt gccaaaaatt cgcagcaaca tcaggaacag gttgatgaag tccagataca
    20701 aggttaacgc gccaagaatg gaatatttgc gcaggttcga cgtgtcgcgg gtatcaatct
    20761 gctcacccat atttttcaat ttctgagtgt catacgccgt caatccgaca aagacaatca
    20821 cgccgatgta ggtaactgcc cacatcaatg cttcgctttt cagccagaag ttgaccagcg
    20881 atgccagcac aatgccgatt aacgccataa acagcatatt gccaaagcca cttaaatcgc
    20941 gcttcgtggt gtaaccgtac aggctcattg cgccgaacat cccggcagta acgacgaaag
    21001 tactggcgat agaagcagcg gtatagacaa tgaatatact ggaaagcgta agacccgtca
    21061 gcgccgaata aagcataaag agcatcgtcg ttacacctgc gctcagcttt tgaatcatcg
    21121 ctgataacac aataaccaat gctaattgcg cgatgatcag accgattaaa aagacacggt
    21181 tagtgaacaa cagctccatc acggccgcgg aattagccgc ataccaggca acaaatgcgg
    21241 tcagcaacaa gccaacggtc atccagccat agacttgcgc catataagtt tgcaagccag
    21301 cccggggttg tacgattgaa tcagaacgtg ggaatctgtc catgacgatc tcctgaagat
    21361 ataaggaata tcttaaggat actgcaaaat gatgaggctg tgcatcgacg cagcgtaaac
    21421 gcatgtactg agcggtgaaa ttgccggacg cagcggtgcc ttatccggct aacaaaaaac
    21481 taccagcgtt ttgccgcctg ctgatcgctc tcccgagctt caacccagcg gtcgccttcc
    21541 ggcgtggctt cgcgcttcca gaacggtgcg cgggttttga gataatccat aataaactgc
    21601 ccggcttcaa acgcactgct gcgatgcgca ctggtgacac cggcaaaaac gatttcatcg
    21661 cccggccata attccccgat gcggtgaatc acagtgacgc gccccagcgg ccagcggtta
    21721 cgcgcttcat caacaatttc tgccagtgct ttttcagtca ttcccggata gtgttcgagg
    21781 gttaatgctt tgacgctgtc gcccaggtta tggttgcgca ccttaccagt aaaggtgact
    21841 accgcaccgt cttcgtcacg ctccgccagc cacgggtact cttctcctac gctgaacggc
    21901 tgcggaccaa caacaatttt ggtttctgcc atcttaacct ccggttaccg gcgggaagaa
    21961 agctacttcg tcgccgtcag tcagcggatg gtcaaaactc accagcgtct ggttgacggc
    22021 agccagtaat ttgccatctt ccagcgccag cgcccagcga tcgctctgcg cagccatgtg
    22081 ctggcgtaac gcttcaacag ttgggaaatc cgcagccact tcggttgcat ctgttcccac
    22141 caactcgcgc acctgcgcga aaaaaagaac tttaatcatc cgcttccacc ttaaagtcac
    22201 ccgacttgcc gccgctcttc gccagcaaac gtaccggacc aatcaccata tctttttgca
    22261 ccgctttgca catgtcataa atggtcagcg ccgccacgga ggccgcggtt aatgcttcca
    22321 tttcgacacc ggttttcccg gtcaggcggc ataaggtttc tatacgcacc cgattgtgct
    22381 ccggctcggc ctgtaaattg acttcaactt tgctgagcat cagcggatga cagagcggga
    22441 tcagatccca ggtgcgtttt gccgcctgaa taccggcaat acgcgcagtg gcaaatacgt
    22501 cgcctttgtg gtggcgacca tcaataatca tcgccagcgt ctcgctgcgc atggtgacaa
    22561 aggcttccgc ccgcgcttca cgcacggttt ccgctttggc ggagacatcc accatgtgcg
    22621 cttcgccagc ggcgttgata tgggtcagtt gcgacatact tatttcttca aatgtggatg
    22681 gaaattacac ggacgcgtac gggcatccag ctgcggcgcg atgatatttt cccatgcggt
    22741 acggcacgct ttggtcgaac ccggcatggc gaaaatcagc gttttgttgg cgacgcccgc
    22801 aaccgcacga gattgcaacg tggaagtgcc aatctcttca aacgacaaca tccggaacac
    22861 ttcaccaaaa ccttcaactt cacggtcgaa caacggcagc aatgcttcgg gagcctgatc
    22921 accttccgtc aggccagtac cacccgtaat caataccact tgtacatcgt cgctggcgat
    22981 ccacgcagat acctgagcgc gaatagcgta gcggttttct ttcacaatgg ctttatcgac
    23041 aacgtgatgg cccgcttctt gcgccgaatc gcgcagatag tgaccggagg tatcgtcttc
    23101 ttcaccgcga cgattagaaa ccgtaagaat agcaatacgg gtcgggataa attcagtgct
    23161 tacctgactc atctgatctc tccttttgac gttttagccg ccaatgtacg ataagttttg
    23221 cgtaataccg gtgttgtttt gatgcaggaa atgggtctgt ttcttctccc gcagcgccgc
    23281 tgaaatacgc gcttccagcg cctgttgctg ggtatcgtct tccagcagat cgcgcaggtt
    23341 aacgccgcct tcaccaaaca ggcagagatg gagtttacca atggaggaaa cgcgcaggcg
    23401 gttgcaagtg gcgcagaagt ctttttcata cggcatgata aggccaatct ctccggcgta
    23461 atctggatgg caaaagactt gcgcgggacc gtcgctgcgt tgacgtaatt ggtggatcca
    23521 gccgcgacgc agtagctcgt cacgcagaac ctgaccagag atgtgatgct tacggaagag
    23581 ctcgctgccc tcgcccgttt ccatcagttc gatgaaacgc agctggatag ggcgatgctg
    23641 gatccagttc agaaaggtgt cgagctggtg atgattaaca tcacgcatca gcacggtatt
    23701 gactttgacc ttctcaaaac cggcctcaaa tgcagcatca atccctgcca tgacctggtt
    23761 gaatttatcc tgcccggtaa tagcgtgaaa ctggcgggcg tccagactgt cgacactgac
    23821 gttaatgcca gtaagtcccg catcgcgcca gttcgccaca tcgcgttcca gacggtaacc
    23881 attggtggtg accgcaatct ggcggatagc gtcgttttcc cgcacagcgg cgatgatatc
    23941 ggtaaagtcg cggcgtaaag acggctcacc tcctgtcagg cgcacttttt cggtgcccag
    24001 actggcgaag gcgcgcgtaa cccggcgaat ttcatcgacg gtaagaaagc ctttattggt
    24061 gacgccgctc ggtttgtagc catccggcag gcagtaggtg caacgaaagt tacacacatc
    24121 ggtaatcgac aggcgcaagt agtaaaactt acgcgcaaat gcatcagtca gttgtgaagc
    24181 catgtacacc tttccagata cgggaggcga agtcatttct tccttcgccc tggtggcaat
    24241 ctttacgagc attgtcacgg ccaaagcacc gtatcagttg acccaggtgc agaggctaga
    24301 gtgtttagtg gttaagccga tactagcgtg taaatgagat ttttaccatc cacactttcg
    24361 ctatgcaatc atgtatatag cgtcatgatc gtgctatttc gctacagaat aagggaaaat
    24421 ggcagtttta ctttgatata catcatcagg gaggcatagc gtgcatcgtc ctttagaggt
    24481 agaagaaaat agtttgtatc ctcaatattg gcaggttaat tgctgtttcc ccgcaatttg
    24541 cgctactgta gcgcggataa tctgactcca ggaaactata tgcgcaatcg tacgctggct
    24601 gatcttgatc gtgtcgttgc tctcggcgga gggcatggac tgggacgcgt tctctcatca
    24661 ctttcgtctt tgggttctcg tttaacgggt atcgtcacca ccacggataa tggtggctcg
    24721 acggggcgta ttcgccgttc agaaggcggc attgcctggg gcgatatgcg caactgcctc
    24781 aaccaactga taacggaacc gagcgtcgcc tccgcgatgt ttgaataccg ttttggcggc
    24841 aatggcgaac tttccgggca caaccttgga aacttgatgt taaaggcgct ggatcacctt
    24901 agcgtgcggc ctctggaagc catcaattta attcgtaatc tgctgaaagt ggatacgcat
    24961 ttgattccaa tgtcagagca tcccgtcgat ctgatggcga ttgacgatca ggggcatgaa
    25021 gtttacggcg aggtcaatat cgaccagtta actacgccga ttcaagagtt attattaacg
    25081 ccgaatgtac ccgcaacgcg tgaggcagtt cacgctatca atgaagcgga tctcatcatt
    25141 attgggcctg gcagttttta taccagcctg atgccaattc tgctgctgaa ggaaatcgcc
    25201 caggcattac gccgcacgcc agcgccgatg gtttatatcg gcaatctggg gcgtgagttg
    25261 agtttacctg cggctaattt gaagctggaa agcaagctgg caattatgga gcagtatgtt
    25321 ggtaaaaaag tcattgatgc ggtcatcgtc gggccaaaag tggatgtctc ggcggtgaaa
    25381 gagcggattg tgatccagga ggtactggag gccagcgata ttccgtatcg tcatgaccgc
    25441 cagttgttac ataacgcgct ggaaaaggcg ttacaggctt taggttaacg gaaatccgaa
    25501 ggaaaattcc ggcttcctat tgaagacaaa gtgcgcgttg tttatgccgg atgcggcgtg
    25561 aacgccttat ccggcctaca aaccgcgcaa attcaatata ttgcggagaa aatgtaggcc
    25621 tgataagcgt agcgcatcag gctgttttcc gtttgtcatc agtcttcttc gctatcctgt
    25681 tacgatgcgg caataaacaa ctcacgcaac tgatgcaact ggtcacgaat ttgtgccgct
    25741 tcttcgaact ccagattctg cgcgtgttgc atcatcagcc cttccagttc atggattttc
    25801 tgctgcaacg ctttcggcga catatccatc ggcacattat ccggctcaac aatcgggcgc
    25861 gattttcctc tgcccttcgc tttggttttg gcaatgttct gccccagcgc caggatatcg
    25921 accactttct tgttcaagcc ttgcggcgta atgccgtgtt cctcgttgta cttctgctgt
    25981 ttctcacggc gacgttcggt ttcgccaatc gctttcgcca ttgatggggt gatcttatcg
    26041 ccgtagagaa tcgctttacc gttaacgtta cgtgccgcac gaccaatggt ctggatcaac
    26101 gaacgttcgg aacgcaggaa gccttctttg tcagcgtcga ggatcgccac cagcgacact
    26161 tccggcatat ccagaccttc gcgcagtaag ttgatcccta ccagcacgtc gaactcaccc
    26221 agacgcaagt cgcggataat ctccatacgt tcgacggtgt cgatatctga gtgaagataa
    26281 cgcacgcgct cgccatgttc ttcgagatat tcggtaagat cttccgccat ccgcttggtc
    26341 agtgtggtga ccagtacgcg ttcgttaatt gccgctcgct gacgaatctc cgaaagaaga
    26401 tcatcaacct gtgtcgccac cggccgcact tcgataatcg ggtcaagcaa tccggttgga
    26461 cgcaccacct gatccaccac atcgccgccg gatttttcca gctcgtaatt acccggcgtc
    26521 gccgaaacat agatggtttg cggcgctaat gcttcgaact cttcaaactt aagcggacgg
    26581 ttatccagcg ctgatggcag gcggaagccg tactccacca gtgtctcttt acgcgcccgg
    26641 tcaccgcgat acatgccgcc aatttgtgga atggtgacgt gagattcatc gacgaccagc
    26701 agcccatcgg caggcaggta atcaaacagc gtcggcggtg gctcacccgg tccacgaccg
    26761 gagaggaagc gcgagtagtt ttcaatcccc gaacagtagc ccagctcgtt catcatctcc
    26821 agatcaaact gggtacgctg ggtcagccgc tgctcttcca gcagtttgtt gttttccaac
    26881 agcactttgc gtctggcggc cagttcttct ttgatctcct ccatcgcctg tacgatgcgc
    26941 tcgcgcggtg tgacgtagtg cgttttcggg tagatggtaa aacgtggaat agtggaaaca
    27001 atctgcccgg tcagcgggtc aaataacgac aatcgttcca cttcctcgtc aaacagttcc
    27061 acgcgaagtg caatgtcatc cgattctgcc gggaagatat caatcacctc gccacgaacg
    27121 cggaaagtac cacgctggaa tgcttgatca ttacgagcgt attgcagctc cgccagtcgg
    27181 cgcagaatcg cgcgctgatc gataatcata ccgaccgtga gatggagcat catcttgaga
    27241 tataaatcag gatcgcccag accataaatc gcggaaacag aggccactac aaccacatca
    27301 cgccgctcca gcatcgcttt ggtggcggat aaacgcatct gctcaatatg ttcgttaacc
    27361 gaggcatctt tctcaatgaa agtgtcggaa ctcggtacat aggcttccgg ctgatagtag
    27421 tcgtagtagg agacgaaata ctccaccgcg ttttccggga agaactcttt catttcgcca
    27481 tacagctggg ccgccagcgt tttgttgggc gcaagtacca tggttgggcg ctgaaggtca
    27541 gcaatgacat tggcaatggt gaaggttttc cctgagccag tcacgccaag taacgtctgg
    27601 tgcgccaggc catcttccag cccctcttcg agacgtcgaa tcgcctctgg ctgatcgcca
    27661 gaaggtttaa aagcggaatt cagtttgaac ggtttactca tgagtcgcta cctgaaggag
    27721 ttgggcgggc aggtatgtaa ttttactcgt cgtacttaat tatgccaaca aattatactg
    27781 gataaaaaaa cagttcatca ccataatatt tctgatacag cgtaaactcc gctgtcaatc
    27841 atgagcaaaa tttactctgt ggcgagataa aactccggcc ttaccgggtt atccccaaag
    27901 caacggcttt tttaacattt gtcaagatga gtgatgacag ttttatggca agagatgcct
    27961 gttcagtgac tgccattgca tttatctaac cagttaaaaa ataaaagata tttctttgag
    28021 ccgtctttaa cgccaattcg cgtgcaagcc gcgtattctc tcgcttgcct cgtgttttct
    28081 aactctaata cacatggtta tccacaggaa tagtggataa ctgtctccag cccctatccc
    28141 ccgccgcctg ggcaattccc acattcgccg catgaggcgg aagtaaaatt tttttcgtga
    28201 ttattttaga atttaattgg ttaaattgca gtcaatcgaa gacgcgatct cgctcgcaat
    28261 ttaaccaaat acagaatggc tacaacaagg caaggtttat gtactttccg gttgccgcat
    28321 tttctggatt ttctgcaagc caggggatct ctcccagcag cggcgcggga atcatgcggg
    28381 tgagcgtggt catatattca gcgtgacgtt ttcccggagg cgtaacatcg ttcgccaccc
    28441 aacccgccag agtcagtccg gcgtgttgta ttgcctgcgc agtcaacatc gcgtgattaa
    28501 tacagccgag tttcacacca actaccagta tcaccggcag ttgttcctgt gttacccaat
    28561 ctgcaaaagt gaaagtgtca gaaagcggcg taaaccagcc gccagcacct tccactaaca
    28621 cccagtcagc ctgttgttca agcgcgcgta atccggcgct cattaccaat gattctatcg
    28681 gcctgccctc ttgcgcgctg atgatgtgcg gcgaagtggg ttctgcgaag gtgtaaggat
    28741 ttactgttgc gtaatccagc tgcaggctgc tgttgtgctg taacgccagc gcgtcactat
    28801 tacgtaagcc ttccggggtc ttttcgctgc cagaggcgac cggtttataa cctgccgtcc
    28861 gataacctac tgccttcgcg gcttgtaaaa gtgcacaact ggcgacggtt ttccccactt
    28921 cggtatccgt tccggtgaca aaataacgtt tactcacgag caatcactcc caaaaaaaga
    28981 tgatacgtca gaggatatcg cccctgttgt tgcggccagg ccagttgcaa tcgctgcaat
    29041 tgcgaacgcg ttaatattcg cgggtcgcgc ccttcatgaa gatgcgtagc accgatgcct
    29101 ttcagcgaac gcatggcact gagcgcatca tcaaaccata gtgtgatggg ctgaatatga
    29161 tgttgataat gcacgccgtt cagcgactgt tcgatttcat ctggcggtaa aaagcgatta
    29221 gcatgcggac gctcgtccac cgcctgccac gcctgatgca gttcgggtaa cgatccctgc
    29281 accagcgtgg taaacgccac cacgcctccg gggcgcacca cccgatacag ctcgcggagt
    29341 gccgtggata aattaccgca ccactgcacc gcgagattgc tccatgcaag atcgaacgtc
    29401 gcagtcgcta acggcaggga ttcgatatct cccgccagat aatggtctgc agcatctttc
    29461 tggcgggctt gcacaagcat tggcggtgag agatctaacg ccgtcacctg tgcgtgacgc
    29521 tctcgccagt ggcggctcat ccagcctggc ccacaccccg cgtccagtac gtgggtgtat
    29581 ttacgctgtg ggagcattgc cagtaagacg tcagcactcc ggcgctgtaa ctctgcgtgt
    29641 tgctcatagt gcgcggctgc ccgaccaaat gccgctgcaa tggcttgttt attaaccgtt
    29701 gccatgcagc acctccagca gacggtcgat atcctgcatt tcatgcgctg cggttagcgt
    29761 taagcgcagt cgcgcagtac cagcgggtac ggttggcggg cgaatcgccg tgacccagca
    29821 gccttgctgg cgcagttttt ctgccagttg taacgcacgg ctgttatcac cgacgatcaa
    29881 tggctggatg gcgctgcatg aatcagcaag cgtaaacggc aaatcctgta ctccggcacg
    29941 aaaacgcgca atgagtgaca ccagtttttc gcgccgtgca tccccctcat cacggcgaat
    30001 gaccgccagc gacgcacgta atgcctgcgc ctgagcgggc ggcatactgg tgttgtagat
    30061 aaggtggcgg gcgaattgca gcagataatc cgccaccgta ctggagcaaa gcaccgctgc
    30121 cccgctgacg ccaaatcctt tgccaaaagt caccaccagc aattctggtt ttaccttttg
    30181 cagccagcag ctgccgcgcc cctgctcccc gataacgccc gtgccgtggg catcatcgac
    30241 catcaaccag ccattgtgct gttgcgttac ctgctggatt tccgccagtg gcgcactatc
    30301 gccgtccatg ctgaacacgc cttctgtcac caccagttgc tgccccggac agggggaagc
    30361 aagcagtcgc gccagatgag tgacatcgtt atgaacaaaa cggcgaagct gcgacgggct
    30421 taaactggca gcctccagca atgaggcatg gctaagccgg tcagcgacaa tgcggtcctc
    30481 ttttgccatc atcgcggcaa tgaccgcctg attagcggca aaaccagaga taaatagcag
    30541 tgcccgcgaa tagcccagcc actcggccag ttcttcttcc agcgcctgat gcgcaacgct
    30601 ataaccgctg acgtgaccgg aaccgccgct accgacgcca aattgctccg caccctgctg
    30661 ccaggcacgg ataatttgcg gatgatggct taaacctaaa taatcgttac tggaaaagtt
    30721 cagatactgg cgatcatccg ccaccagcca gcgtccggct ccttgcgcca ctggataacg
    30781 gcgacgcagg gcatcggcag cacgccgcgc atcgagcgcc gcgttgattt tctcctgcca
    30841 gctcatagtg ctgccgcgtt gtaatattcg tcagtatccg gggtcatcag cgcctgttca
    30901 agacgttgct gttgttcgtt atcccctgcc agcacggcag tttgctgcgg atttagcccc
    30961 agtttgcgga acagttgcag gtctttatct tcttccggat tcggcgtggt cagcagtttg
    31021 caaccgtaga aaatcgagtt tgcgcctgcc ataaagcaca tcgcctgagt ctgttcgttc
    31081 atctgctcgc gtccggcaga aaggcgcacg taagaggtcg gcatcatgat ccgcgcgacc
    31141 gcaatggtgc gaataaaatc aaaggcatca acgtcatcgt tatcggcaag cggcgtgcct
    31201 ttcaccttca ccagcatgtt gattggcacg ctttccggcg gcgtcggcag gtttgccagt
    31261 tgcagcaata atccggcgcg atcttttacc gtttcgccta agcccacaat gccgccagaa
    31321 cagactttga tcccggcatc gcgcactttt tccagcgtat cgaggcgttc ctgataagtg
    31381 cgggtggtga tgatattgcc gtaaaactcc ggcgaggtgt cgaggttgtg gttgtagtaa
    31441 tccagcccgg cgttcgcgag gcgctgcgcc tgagattcac tcaacgtgcc cagcgtcata
    31501 cacgcctcca gccctaaatc ttttaccccc tgcaccatct gctccaggta cggcatatcg
    31561 cgttcgttgg gattcttcca cgccgcgccc atgcaaaaac gcgtcgatcc cgccgctttc
    31621 gctttgcgcg ccgactccag cacctgttca acttccatca accgctcggc ttccagaccg
    31681 gttttatagc gcgagctttg cgggcaatat ttgcaatctt ccgggcaagc accggtttta
    31741 atcgacagca acgtgctgac ctgcacctga cgaggatcga aatgctggcg atgcacctgc
    31801 tgcgcttcaa acagcagatc cagcaacggt ttttcaaata attctgcgac ttgcgacaat
    31861 gtccagcgtg ggcggtgagc catggggctt ctccaaaacg tgttttttgt tgttaattcg
    31921 gtgtagactt gtaaacctaa atcttttcaa tttggtttac aagtcgatta tgacaaagga
    31981 cgatcttgcc tttgaccaac gccatatctg gcacccatac acatccatga cctccccgct
    32041 gccggtttat ccggtggtga gcgccgaagg ttgcgagctg attttgtctg acggcagacg
    32101 cctgattgac ggtatgtcgt cctggtgggc ggcgatccac ggctacaatc acccgcagct
    32161 taatgcggcg atgaagtcgc aaattgatgc catgtcgcat gtgatgtttg gcggtatcac
    32221 ccatgcgcca gccattgagc tgtgccgcaa actggtggcg atgacgccgc aaccactgga
    32281 atgcgttttt ctcgcggact ccggttccgt agcggtggaa gtggcgatga aaatggcgtt
    32341 gcagtactgg caagccaaag gcgaagtgcg ccagcgtttt ctgaccttcc gcaatggtta
    32401 tcatggcgat acctttggcg cgatgtcggt gtgcgatccg gacaactcaa tgcacaacct
    32461 gtggaaaggc tatctgccag aaaacctgtt tgctcccgcc ccgcaaagcc gcatggatgg
    32521 tgaatgggat gaacgcgata tggttggctt tgcccgcctg atggcggcgc atcgtcatga
    32581 aatcgcggcg gtgatcattg aaccgattgt ccagggcgca ggcgggatgc gcatgtacca
    32641 tccggaatgg ttaaaacgaa tccgcaaaat gtgcgatcgc gaaggtatct tgctgattgc
    32701 cgacgagatc gccaccggat ttggtcgtac cggcaaactg tttgcctgtg aatatgcaga
    32761 aatcgcgccg gacattttgt gcctcggtaa agccttaacc ggcggcacaa tgaccctttc
    32821 cgccacactc accacgcgcg aggttgcaga aaccatcagt aacggcgaag ccggctgctt
    32881 tatgcacggg ccaactttta tgggcaatcc gctggcctgc gcggctgcaa acgccagcct
    32941 ggcgattctc gaatctggcg actggcagca acaggtggcg gctattgaag tacagctgcg
    33001 cgagcaactt gcccccgccc gtgatgccga aatggttgcc gatgtgcgcg tgctgggggc
    33061 aatcggcgta gtcgaaacaa ctcgtccggt gaatatggcg gcgctgcaaa aattctttgt
    33121 cgaacagggg gtatggatcc ggccttttgg caaactgatt tacctgatgc cgccctatat
    33181 tattctcccg caacagttgc agcgtctgac cgcagcggtt aaccgtgcgg tgcaggatga
    33241 aacatttttt tgccaataac gggaagtcag cgtgtgggtt tctggctaca ctttctgcaa
    33301 actagaaagg agggttcatg aaactcatca gtaatgatct gcgcgatggc gataagctgc
    33361 cgcatcgtca tgtctttaac ggcatgggtt acgatggcga taatatttca ccgcatctgg
    33421 cgtgggatga tgttcctgcg ggaacgaaaa gttttgttgt cacctgctac gacccggatg
    33481 cgccaaccgg ctccggctgg tggcactggg tagttgttaa tttacccgct gatacccgcg
    33541 tattaccgca agggtttggc tctggtctgg tagcaatgcc agacggcgtt ttgcagacgc
    33601 gtaccgattt tggtaaaacc ggatacgatg gcgcagcgcc gccgaaaggc gaaacccatc
    33661 gctacatttt taccgttcac gcgctggatg tagagcatat tgatgtcgat gaaggagcca
    33721 gcggcgcgat ggtcgggttt aacgttcatt tccactctct ggcaagcgca tcgattaccg
    33781 cgatgtttag ttaatcactc tgccagatgg cgcaatgcta tctggtatca cttaaaggta
    33841 ttaaaaacaa ctttttgtct ttttaccttc ccgtttcgct caagttagta taaaaaagca
    33901 ggcttcaacg gattcatttt tctatttcat agcccggagc aacctgtgaa cacattttca
    33961 gtttcccgtc tggcgctggc attggctttt agcgtgacgc tgaccgcctg tagctcaaca
    34021 ccacccgatc aacgtccttc tgatcaaact gcgcctggta cctcttctcg cccgattctg
    34081 tcggcaaaag aagcgcagaa tttcgatgct caacactatt ttgcatccct gacaccaggt
    34141 gctgcagcgt ggaatccttc cccgattacc ctgcctgcgc aacctgactt tgttgtcggc
    34201 ccggcgggca ctcaaggtgt aacgcatacc acgattcagg cggcggtaga tgcggcaatt
    34261 atcaagcgta ccaacaagcg ccagtatatt gccgtgatgc ctggtgagta tcagggaacg
    34321 gtgtatgtcc ctgctgctcc gggtggaatt actctgtacg gtacaggtga aaaaccgatt
    34381 gatgtgaaga ttgggctttc ccttgatggg ggcatgagcc ctgccgactg gcgtcacgac
    34441 gtcaacccgc gcggcaaata tatgccaggt aaaccagcgt ggtatatgta cgatagctgc
    34501 cagagcaaac gcagcgacag tatcggtgtt ctctgctctg cggtcttctg gtcacaaaac
    34561 aatggcctgc aactgcaaaa cctgaccatc gaaaacacgc tgggcgatag cgtagatgca
    34621 ggtaaccatc cggcggtggc actgcgtact gatggtgacc aggtacagat taacaacgtt
    34681 aacattctcg gttgtcagaa caccttcttt gtcaccaaca gcggtgtgca gaaccgtctg
    34741 gaaacgaatc gtcagccgcg tacgctggtg accaacagct acattgaagg ggatgtggat
    34801 atcgtttctg gtcgcggcgc agtggtgttc gataacaccg aattccgcgt ggtgaactca
    34861 cgtactcagc aagaagcgta tgtgtttgca ccggctacgc tgtccaacat ttactacggt
    34921 ttcctcgccg taaacagccg tttcaatgct ttcggtgatg gtgtggcgca actgggccgc
    34981 tcgctggatg ttgatgccaa taccaacggt caggtggtga tccgtgatag cgccatcaac
    35041 gaaggtttta acacggctaa accgtgggcc gatgcggtga tctctaatcg tccgtttgcg
    35101 ggtaataccg gcagcgtaga tgataacgac gaaatacagc gcaatctgaa tgacactaac
    35161 tacaaccgca tgtgggaata caataaccgc ggcgtgggta gtaaagtggt tgcagaggcg
    35221 aagaagtaag agcaattaac tatttgccgg atgcggcgta aacgccttat ccggcctacg
    35281 gttcgatgcg atttgtaggt cggataagat gcgcaagcat cgcatccgac aataagtgcc
    35341 gaatgcgacc tacattcaca tggcgctttt tacatctgac ggtttttatt gaagttaatc
    35401 aaactacccg ccttgataat ctcgcgctct tcagcagtca gactttccat atagagcgta
    35461 atttccgtta ccggcgcatc ttcatggatc acataacctt taaacgtcgt acccggatta
    35521 tccagcgccg ctttaatgcc agggatgtaa atgtaatccc ccacttcaaa ggttggcact
    35581 tccgccatct gcagcggtaa catcccccag ttgatgacgt tagaacggta gcgtttagtc
    35641 gcgtactctt cggcaatatt cgccagaccg ccaatcacac gctggcagct cgccgcctgt
    35701 tcacgcgcag aaccatcgcc tggtttcacc gcatatacca tgctgccaat ttcagtttgc
    35761 agcggatcaa tatgctcctg accagcaatc tgcttaatgc gcgcaaacac ctctgtcagc
    35821 tcgctgacat tccccgccag acgctgattt tccagctcag ccgtcgcttt acttctgcca
    35881 acataaccgg gatcgcggcg tgacagggta aactccgcca gaccaatcgg atttgaacga
    35941 taagaagagg tttcaccgga aggaatcagt tcgtcggtgg tggtcacttc gtcgaggatc
    36001 ttcgagcaca ctttcaggac gatattgtca gtcagcgcac ccaattccgg ccagtcttta
    36061 atgttcggtc cgtaaatcag cggttgctga gttgcccctt tcacaaagcc ctgataaaca
    36121 cggtttttat acggcgttac atcgaaggcg tactccggca cgttgtccca gcaatcaagt
    36181 tcgctggcag aggttaaata gccaccgttt gccgcagtcg cagcgataga acgagcgtcc
    36241 atcaacgcca ccgctgacat ctgcccatta gctggcttag agccttcgcg gttcgggaag
    36301 ttacgcgtgg tgtggcgaat acttaatccg ttgttgattg gcgtatcgcc cgcgccaaag
    36361 catgggccgc agaacgcggt tctgatgatt gcgcctgcgc caatcaaatc tgctaccaca
    36421 ccttttttgg ctaaatccat aaacaccggc tgtgatgacg ggtaaaccgc cagcgagaag
    36481 gtgtcattgc cacaggattg accgcgcagt gcattcgccg ccgcgatgac gttttcgtag
    36541 ttaccgccag aacagcccgc gataatcccc tgctgcactt tcaggcgacc attttccact
    36601 ttatccagca gcgagagttt ggctttaccg tgcgccacgc gttcggactc aatttcaatc
    36661 tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga tttcatacac gttgctcggg
    36721 tggaacggca gcgcaatcat tggtttgatg gcgcttaaat caacgctgat gcagccatcg
    36781 tagtacgcca tcggttgagg gttaagctgg cagtaatcct ggccgcgacc gtgcagcgcc
    36841 agccagttat gcacttcttc atcagtttgc cagacggagc ttaaacaggt ggtttccgtg
    36901 gtcatcacgt caacgctgtt acggaaatcg gtagagagcg cgctaacgcc cggtccaacg
    36961 aactccatga ctttgttttt gacgtaaccg tttttgaaca ccgcgccaat gatagccagc
    37021 gccacatcct gtggccccac atacggcgca ggttttccgg tcagatgcac cgcaaccacg
    37081 cccggatagt cgatatccca ggtgtcatta agcagctgtt ttaccaactc accgccgccc
    37141 tcaccgactg ccattgtccc taatgcaccg taacgggtgt ggctgtctga cccgaggatc
    37201 attttgcccc cgcctgccat catctcacgc atatattgat ggatgatcgc aatatgcgga
    37261 ggcacaaaaa taccgccata acgctgagct gccgataaac caaaaacatg gtcatcaccg
    37321 ttaatggtgc cgccgacggc gcagagtgag ttatggcagt tggtcagcac atacggcagc
    37381 gggaaacgtt ccattccgga cgctttagcg gtctgtacaa taccaacaaa ggtaatatcg
    37441 tgagaggcta atgaatcaaa cttaatttta agtttatcca tatttccgga cgtattatga
    37501 gaagagagaa tagaccaggc aatagtgcct tttttggctt cttctttttt aatttcgccg
    37561 gtgaaatgtt cttcggcaat tatttcgtta ttactggcga gaaacacgcc ttttt
//
LOCUS       DAJTOR010000017        36293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTOR010000017 DAJTOR010000000
VERSION     DAJTOR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.017"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..896
                     /locus_tag="OO249_000790"
     CDS             <1..896
                     /locus_tag="OO249_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5635184.1"
                     /translation="DIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEEQ
                     IGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNTY
                     PTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELENL
                     TISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGINP
                     TIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIR
                     NHRYTATMLQHIAEDRDGTNH"
     gene            complement(893..2370)
                     /gene="scpC"
                     /locus_tag="OO249_000791"
                     /pseudo
     CDS             complement(893..2370)
                     /gene="scpC"
                     /locus_tag="OO249_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(2394..3179)
                     /gene="scpB"
                     /locus_tag="OO249_000792"
     CDS             complement(2394..3179)
                     /gene="scpB"
                     /locus_tag="OO249_000792"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5635185.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(3190..4185)
                     /gene="meaB"
                     /locus_tag="OO249_000793"
     CDS             complement(3190..4185)
                     /gene="meaB"
                     /locus_tag="OO249_000793"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5635186.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKRTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(4178..6322)
                     /gene="scpA"
                     /locus_tag="OO249_000794"
                     /pseudo
     CDS             complement(4178..6322)
                     /gene="scpA"
                     /locus_tag="OO249_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(6526..7419)
                     /gene="argP"
                     /locus_tag="OO249_000795"
     CDS             complement(6526..7419)
                     /gene="argP"
                     /locus_tag="OO249_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5635187.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7802..8461
                     /gene="rpiA"
                     /locus_tag="OO249_000796"
     CDS             7802..8461
                     /gene="rpiA"
                     /locus_tag="OO249_000796"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5635188.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8716..9948
                     /gene="serA"
                     /locus_tag="OO249_000797"
     CDS             8716..9948
                     /gene="serA"
                     /locus_tag="OO249_000797"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5635189.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            10177..10236
                     /gene="ibsC"
                     /locus_tag="OO249_000798"
     CDS             10177..10236
                     /gene="ibsC"
                     /locus_tag="OO249_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5635190.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10569..10628
                     /locus_tag="OO249_000799"
     CDS             10569..10628
                     /locus_tag="OO249_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5635191.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10729..11331)
                     /locus_tag="OO249_000800"
     CDS             complement(10729..11331)
                     /locus_tag="OO249_000800"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5635192.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHELKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11352..11535)
                     /gene="ssrS"
                     /locus_tag="OO249_000801"
     ncRNA           complement(11352..11535)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO249_000801"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11577..11906)
                     /gene="zapA"
                     /locus_tag="OO249_000802"
     CDS             complement(11577..11906)
                     /gene="zapA"
                     /locus_tag="OO249_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5635193.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            12074..12652
                     /gene="ygfB"
                     /locus_tag="OO249_000803"
     CDS             12074..12652
                     /gene="ygfB"
                     /locus_tag="OO249_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5635194.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12678..14003
                     /gene="pepP"
                     /locus_tag="OO249_000804"
     CDS             12678..14003
                     /gene="pepP"
                     /locus_tag="OO249_000804"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5635195.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            14000..15178
                     /gene="ubiH"
                     /locus_tag="OO249_000805"
                     /gene_synonym="visB"
     CDS             14000..15178
                     /gene="ubiH"
                     /locus_tag="OO249_000805"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5635196.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            15201..16403
                     /gene="ubiI"
                     /locus_tag="OO249_000806"
                     /gene_synonym="visC"
     CDS             15201..16403
                     /gene="ubiI"
                     /locus_tag="OO249_000806"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5635197.1"
                     /translation="MQCVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16477..16560)
                     /locus_tag="OO249_000807"
     CDS             complement(16477..16560)
                     /locus_tag="OO249_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635198.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16850..17944
                     /gene="gcvT"
                     /locus_tag="OO249_000808"
     CDS             16850..17944
                     /gene="gcvT"
                     /locus_tag="OO249_000808"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5635199.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17968..18357
                     /gene="gcvH"
                     /locus_tag="OO249_000809"
     CDS             17968..18357
                     /gene="gcvH"
                     /locus_tag="OO249_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5635200.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18476..21349
                     /gene="gcvP"
                     /locus_tag="OO249_000810"
     CDS             18476..21349
                     /gene="gcvP"
                     /locus_tag="OO249_000810"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5635201.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYL"
     gene            21615..22358
                     /locus_tag="OO249_000811"
                     /pseudo
     CDS             21615..22358
                     /locus_tag="OO249_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(22415..23848)
                     /gene="bglA"
                     /locus_tag="OO249_000812"
     CDS             complement(22415..23848)
                     /gene="bglA"
                     /locus_tag="OO249_000812"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5635202.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23893..24204
                     /locus_tag="OO249_000813"
     CDS             23893..24204
                     /locus_tag="OO249_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR5635203.1"
                     /translation="MQPNDITFSQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24368..25027
                     /locus_tag="OO249_000814"
     CDS             24368..25027
                     /locus_tag="OO249_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5635204.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVELVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSMCHFLAIYLYIGQ
                     A"
     gene            complement(25223..26203)
                     /gene="ygfZ"
                     /locus_tag="OO249_000815"
     CDS             complement(25223..26203)
                     /gene="ygfZ"
                     /locus_tag="OO249_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5635205.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAADDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26446..26712
                     /gene="sdhE"
                     /locus_tag="OO249_000816"
     CDS             26446..26712
                     /gene="sdhE"
                     /locus_tag="OO249_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5635206.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26693..27100
                     /locus_tag="OO249_000817"
     CDS             26693..27100
                     /locus_tag="OO249_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR5635207.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(27140..27661)
                     /gene="fldB"
                     /locus_tag="OO249_000818"
     CDS             complement(27140..27661)
                     /gene="fldB"
                     /locus_tag="OO249_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5635208.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27773..28669
                     /gene="xerD"
                     /locus_tag="OO249_000819"
     CDS             27773..28669
                     /gene="xerD"
                     /locus_tag="OO249_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5635209.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28694..29404
                     /gene="dsbC"
                     /locus_tag="OO249_000820"
     CDS             28694..29404
                     /gene="dsbC"
                     /locus_tag="OO249_000820"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5635210.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            29410..31143
                     /gene="recJ"
                     /locus_tag="OO249_000821"
     CDS             29410..31143
                     /gene="recJ"
                     /locus_tag="OO249_000821"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5635211.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31234..32332
                     /gene="prfB"
                     /locus_tag="OO249_000822"
     CDS             join(31234..31308,31310..32332)
                     /gene="prfB"
                     /locus_tag="OO249_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5635212.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32342..33859
                     /gene="lysS"
                     /locus_tag="OO249_000823"
     CDS             32342..33859
                     /gene="lysS"
                     /locus_tag="OO249_000823"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5635213.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33902..34450)
                     /gene="idi"
                     /locus_tag="OO249_000824"
     CDS             complement(33902..34450)
                     /gene="idi"
                     /locus_tag="OO249_000824"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5635214.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34505..34576
                     /locus_tag="OO249_000825"
     CDS             34505..34576
                     /locus_tag="OO249_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635215.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34573..34698)
                     /locus_tag="OO249_000826"
     CDS             complement(34573..34698)
                     /locus_tag="OO249_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635216.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34700..36148)
                     /gene="uacT"
                     /locus_tag="OO249_000827"
     CDS             complement(34700..36148)
                     /gene="uacT"
                     /locus_tag="OO249_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5635217.1"
                     /translation="MSAIDSQLLSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSVGL
                     SIIQVGIDWAAGGKGNPQYGNPVYSGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDALWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         8719 a         9157 c         9725 g         8692 t
ORIGIN      
        1 tggacatttt tatttcgaaa aagatgcgca atttcatttt attagcgcaa accaataata
       61 ttgcccgggc ggcagaaaaa atccatatga cggcttcgcc atttggtaaa agcattgccg
      121 cgctggaaga acaaattggc tatacgctat ttacccgcaa agataataat atcagcctca
      181 ataaagccgg tcaggaactg tatcaaaaac tgttcccggt ttatcaacga ctttctgcga
      241 ttgataatga aatccataac tcagggcgtc gttcacgaga aattgtgatt ggcatagata
      301 atacttatcc gacaattatt ttcgatcagt tgattagtct cggagataag tatgaaggcg
      361 tgaccgccca gccagttgag ttcagcgaaa acggcgttat cgataatctt tttgaccgtc
      421 agttagattt tattatctcc ccacagcatg tatctgcacg agttcaggaa ctggaaaacc
      481 tgactatcag tgaattacca ccgctgcgat tgggctttct cgtttctcgc cgttatgaag
      541 agcgtcaaga gcaagagctg cttcaggagc tgccctggct acaaatgcgc tttcaaaacc
      601 gggctaactt cgaagcaatg attgatgcga atatgcgacc ttgtgggatc aatcccacca
      661 ttatttatcg tccctacagt tttatggcca aaatcagcgc cgtggagcgc ggacattttc
      721 tgacggtgat cccgcatttt gcctggcgac tggtgaatcc ggcaacgctg aaatattttg
      781 atgcgccgca caggccgatg tatatgcagg aatatcttta ttcaatcaga aatcatcgtt
      841 ataccgccac gatgcttcag catattgctg aagatcgtga cgggacaaac cattaaccca
      901 gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa gacgtggcta agatcgtggt
      961 gaatatgtcc gccgggcgca ttttccagat agcgatgcag ataatcccga tacataggat
     1021 gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg ggaaagaccg cgcagatcgg
     1081 cgatcccttg ttcggtgatg atcactttga cgctgtgttc gctgtgatca acatggctgc
     1141 acattggcac gacggttgag atcttgcctt ctttagcaat cgacggagcc ataaagatcg
     1201 acagatacgc gttgcgttca aaatcaccgc tgccgccgat gccgttcatc agatcgaccc
     1261 cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa ctccaggccg acgttcagag
     1321 cgatgacgcc cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca
     1381 caatgcggct ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga
     1441 ttgtcaggct ggaggcgctg gccccgctga ttttgtcggt ttccagtaaa tgcaccaccg
     1501 attcctgtag cacttccgaa tacatcatga acggaggaat ttccgggttt tcccccagac
     1561 gcgccattac cgcattattg atattgccca cgccactttg cagcggcaga aattccggcg
     1621 gaatacgcca tgcgccattt cctgcaataa gaacgtgacc acgttatcgg caatctgctg
     1681 gcacatggga ttttgcttat ccagcatatt accggcgtcg ggcaagttgg tttccacgac
     1741 ggcgacaatc tttttcggat cgatttgcac atagcgggta ccgacgcgat ccattgcatg
     1801 gaagatcgac acgctattgc gccgcggtgg cgcgccagga atcacaatat ctgccagttc
     1861 tgcaacgcgc ggatcgtgat agtgattaag ttcaatgatc actttcttcg ctcgcagcag
     1921 ccaggtcggc gcattaccga tcccgctggt taaccagact cgaccatccg gtgccagtgc
     1981 cgatgcttca atgacggcaa catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc
     2041 cacttcgctc aaatgcaggt caacgaaact caccgcgccc tgattgatct ttttacgtaa
     2101 accggacgat gtttgatatg gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc
     2161 gtcagcggcg gcgctgattg acgcccccgt cagcaggcga atttgatacg gctttttggc
     2221 ctcatgctgt tcgttagctc tgcgggcaat cgcggtgggt agggctttcg gcgaacccgc
     2281 cggggtaaag ccgctaaatg ccaccatgtc gttatgctgg ataatttctg ccgcttcatc
     2341 ggcggtcatc cttgtccact gagtttccat ttttactcct tcgttcgcag ggattaatga
     2401 ccaacgaaat taggtttacg tttttcgagg aaagcgttca ttccttcctg gtaatcttcg
     2461 ctgtcataaa ccgcgcgtcg catcccctga atacgttcaa attcatcgga gttcatggtg
     2521 tgcgcttcgc ccagtacacg cagctcttct ttgataacag caatggctaa cggcgctttc
     2581 tcagagatgt ggtgcgccat ttgtaaggtg aaatcttcca gttcttccac ttccacaaca
     2641 tggttgagga tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc
     2701 agctctttga caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta
     2761 tacgggacgc cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc
     2821 agatcggaac tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa
     2881 atgatcggtt tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag
     2941 gagagcggat cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact
     3001 ttggatccac tcggcgcgcg caaaatgata cagcgaattt ccggccggtt gagatcgctt
     3061 aacgcctgca taagatcatc aataaagact ttacttaagg cattaagttt tcggccatag
     3121 ttaaactcaa tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata
     3181 aaaattcctt taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt
     3241 gcgcggtgag agcgtattgt ttttgaccgc taaaagcgtc tggcggtaat agcgatcgaa
     3301 atcttcattc gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc
     3361 caccgattgt tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa
     3421 ctcgatgatg gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag
     3481 aacccgtggc tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat
     3541 atgccgggca atggcgacat tggtatggtt atcgccatcg tctttgttga taacgatcag
     3601 atcagccact tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat
     3661 ttgcaacgag ataaaacagt ccaccatgcg ggcgacttct gtttccgact gcccgacgcc
     3721 aaccgtttcg acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg
     3781 cgctcgctga ctggcaccgc ccagatgacc ggaggatggt accgggcgaa taaacgccgc
     3841 ttcggcacgc gccaggtcat tcatgcggat tttatccccg agaatgctac cgccagtgac
     3901 cgggctgctg ggatcgaccg caataaccgc gacctttaat ccctctcgaa tcaacaacat
     3961 gccaaaggcc tcaagaaagg ttcttttccc cgcgctgggg gtgccggtaa cgcccagtcg
     4021 cagggtgtta ccgcagtacg gcataatggc atcaagcagc tgcgtactta gtgcctgatg
     4081 acgcgggtga cggctttcca ccagcgtcat ggcctgggcg agtgtggcac gctcaccctg
     4141 acgtaagcgg cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca
     4201 gattcagtac gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca
     4261 cgccgcgctc ttgcaggaag gcgtaatcct gcggtggaat gacgccaccg gcgactacgc
     4321 agatatcttc gcgtccccat tttttcagcg cctcgaccag ttccgggatc agcgttttat
     4381 gaccggcagc cagtgaggat gcgcccacta cgtgaacgtc attttctact gccaggcggg
     4441 cgatctcttc aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat
     4501 aggcgctggc gatcactttc gagccacgat cgtgcccatc ctggcccatt ttggcgatca
     4561 gaatgcgcag gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat
     4621 cgaactcgga ggccgatttc tcagattgat gatagctttg cgcaatcacg ccggtaacac
     4681 actggcttgg caccagataa cggtcgaaag cggcttccag cgcatcggaa atttcaccca
     4741 gggtggcgcg aacgcgagcg gcattaacag cggcagccag caggttttcg ttatgctgtg
     4801 cggcgtgagt cagggcgttc aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa
     4861 tgcgttccag cgaagcaatt tgctcgttac gcaccatcac gttgtcgatc tcaagtacat
     4921 cggtttcgtc ttcgtgatcc agtttgtact tgttgacacc aacgatgaca cgtttgccct
     4981 ggtcgataag cgactgttcg cgcgctgagg cctattcgat cattcgtttt ggcagacctg
     5041 cttcgatcgc tttcgccata ccaccggctt cgtcgatctg ttggataata gctctggctt
     5101 gtttgacgat ttgatcggtc agcgactcaa tgtaatagga tccggccagt ggatcgacgg
     5161 tgcggcagag ttctgattct tcctggatga tgatctgggt gttgcgggca atgcgtgctg
     5221 agaaatcggt aggcaaacca agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac
     5281 cgcccagcgt cgcagccagt gcttcaatgg tggtgcggat aacgttgtta tacggatcct
     5341 gttcagtcag gctccagcct gaggtctggc agtgggtacg cagcgccagt gatttcgggt
     5401 cctgtgcgcc aaatccactg accgcttcgc tccataaata acgtgccgca cgcaacatgg
     5461 cgacgttcat aaacagatcc atgccgatgc cgaagaagaa cgacaggcga ggagcgaagt
     5521 catcaatttt cagtccggca gagattgctg ctttgatgta ctcaatccca tcagcgagcg
     5581 taaatgctac ctgctgcacg cagttggcac ccgcttcacc catgtggtaa ccgctgatac
     5641 tgatggtatt aaatcgcggc atgttgccgg aacaccaggc gatgatgtcg gcgataatgc
     5701 gcattgacgg ttttggcggg taaatatagg tgttgcggca gaggtactct ttgaggatat
     5761 cgttctgaat ggtgccggtc agtttatcag gtgtaacacc ttgctcttcc gcggcgacga
     5821 tataaaacgc cagtactggt agcactgcgc cattcatggt catcgaaacc gacattttat
     5881 ccagcgggat ctggtcgaac aggactttca tatcttccac ggtgtcgata gcgacgcccg
     5941 ctttgccgac gtcgcccgcc acgcgcgggt tatcggagtc gtagcctcgg tgggtggcaa
     6001 ggtcaaacgc aacggaaaga cctttttgcc cggcggccag gttacggcga taaaaagcgt
     6061 tggactcttt tgctgttgaa aaaccagcat actgacggat ggtccacggt tgggcggtat
     6121 acatagtggc acgcgggcca cgaacgtagg gcggcaaacc aggaagggta cctgtcacct
     6181 ccagattatc gagatcggct tcggtataca gcggcttgat ggtgatccct tccgcggttt
     6241 gctgaaccag cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt
     6301 gccactcctg cacgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt
     6361 gcgcctacgg tgagcttttt acgtgggttt gtcgccagca aaaaaaatgc attcagacgt
     6421 cgttttttgc cgcctgagat ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa
     6481 gtgaggacaa cgcctgatat gtgctatcag gcattatttg atggattaat cctgacgaag
     6541 gactttgtga ccataatcga gcaacgcatc agtgacttta cgcatcatgc ggctttcagg
     6601 agcaaagcgg tgccagtaga gcatccggcg ttgaaatagc cctggcgtta agtcaatcag
     6661 ttcaccgctg gctagttctt tctcgatttg caggtgcggg atcatacagc aggtggtgcc
     6721 ctggcgagca agttgcacga acgcttctga agaattaacg atatggcagg gcacgctgcc
     6781 tggaggcaga tcgaagtttt gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa
     6841 ggcgaccaca ggtgctttca ataatgccga acgcgttacg ccgttaggga aatatttttc
     6901 ggcaaacggt tttgagctga cgaacagata gtcaagcgca ccgagcttat cgacaaggca
     6961 actcggcagc gcctgatgtt gaatactcac cgcgccgacc acttcgccgc ggcgcagacg
     7021 ttcctgagta cgggtttcat cttctacctg caagttgagg cggataggcg aatcagccaa
     7081 tacaggagcc agtgccggaa gcaaccacgt cgccagactg tcggcgttga ccgccagtga
     7141 aagcagcagc ggagtcgaac cggtttgttc atcgcccagc cactcttctt ccagcaactc
     7201 cacctggcgc agcagtgcca gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt
     7261 acgcaccaac agcggctgcc cgaacatatt ttccagttgc ttaatgcgct gtgagacggc
     7321 tgattgtgta atgcacagct tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc
     7381 atccagtgcc tgtaatgttc tgtagtccgg gcgtttcatt gctgttgtct gctcctggta
     7441 aatgcttttt cctgcactat gacacaattt tgtgtcaggt tgcatatacc gcagccgtcg
     7501 gcgggtaaca tttgtatcaa tggttaaacg cgcagcttaa aagatcaggg atttgcgcta
     7561 ttgcatttcg cttagcgact tcgcgccctg gttatgttga atggcgtggc gttattgcct
     7621 ctgttcgcac cgtggagcgg catacccaga ccgttgtatg ctttatttgc ggggcgaaag
     7681 gggatgcccg ccattgcgcg atattttatg gatgagttaa ccacccggct tgccaacggg
     7741 gtctgaatcg ctttttttgt atataatgcg tgtgaaattt cataccacag gcgaaacgat
     7801 catgacgcag gatgaattga aaaaagcagt aggatgggcg gcacttcagt atgttcagcc
     7861 cggcaccatt gttggtgtag gtacaggttc caccgccgca cactttattg acgcgctcgg
     7921 cacgatgaaa ggccagattg aaggggccgt ttccagttca gatgcttcca ctgaaaaact
     7981 gaaaagcctc ggcattcacg tttttgatct caacgaagtc gacagccttg gcatctacgt
     8041 tgatggcgca gatgaaatca acggccacat gcaaatgatc aaaggcggcg gcgcggcgct
     8101 gacccgtgaa aaaatcattg cttcggttgc ggaaaaattt atctgtattg ccgacgcctc
     8161 caagcaggtt gatattctgg gtaaattccc gctgccagta gaagttatcc cgatggcacg
     8221 tagtgcagtg gcgcgccagc tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt
     8281 ggtgaccgat aatggcaacg tgatcctcga cgtccacggc atggaaatcc ttgacccgat
     8341 agcgatggaa aacgccataa atgcgattcc tggcgtggtg actgttggct tgtttgctaa
     8401 ccgtggcgcg gacgttgcgc tgattggcac acctgacggt gtcaaaacca ttgtgaaatg
     8461 atctgacggg ggaacctccc ccgttaaaaa aattctcttc attaaatttg gtgacatgtg
     8521 tcacgttttt acctggctat tgtcgattgc tctaaataaa tcctctaaac cagcatattc
     8581 aaccaagaaa tacctttgcg tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc
     8641 cgcaatatta ttttttgata tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa
     8701 gacaggattg ggtaaatggc aaaggtatcg ctggagaaag acaagattaa gtttctgctg
     8761 gtagaaggcg tgcaccaaaa ggcgctggaa agccttcgtg cagctggtta caccaacatc
     8821 gaatttcaca aaggcgcgct ggatgatgaa caattaaaag aatccatccg cgatgcccac
     8881 ttcatcggcc tgcgatcccg tacccatctg actgaagacg tgatcaacgc cgcagaaaag
     8941 ctggtcgcta ttggctgttt ctgtatagga acaaaccagg ttgatctgga tgcggcggca
     9001 aagcgcggga tcccggtatt taacgcaccg ttctcaaata cgcgctctgt tgcggagctg
     9061 gtgattggcg aactgctgct gctattgcgc ggcgtgccgg aagccaatgc taaagcgcac
     9121 cgtggcgtgt ggaacaaact ggcggcgggt tcttttgaag cgcgcggcaa aaagctgggt
     9181 atcatcggct acggtcatat tggtacgcaa ttgggcattc tggctgaatc gctgggaatg
     9241 tatgtttact tttatgatat tgaaaataaa ctgccgctgg gcaacgccac tcaggtacag
     9301 catctttctg acctgctgaa tatgagcgat gtggtgagtc tgcatgtacc agagaatccg
     9361 tccaccaaaa atatgatggg cgcgaaagaa atttcactaa tgaagcccgg ctcgctgctg
     9421 attaatgctt cgcgcggtac tgtggtggat attccggcgc tgtgtgatgc gctggcgagc
     9481 aaacatctgg cgggggcggc aatcgacgta ttcccgacgg aaccggcgac caatagcgat
     9541 ccatttacct ctccactgtg tgaattcgac aacgtacttc tgacgccaca cattggcggt
     9601 tcgactcagg aagcgcagga gaatatcggc ctggaagttg cgggtaaatt gatcaagtat
     9661 tctgacaatg gctcaacgct ctctgcggtg aacttcccgg aagtctcgct gccactgcat
     9721 ggtggtcgtc gtctgatgca catccacgaa aaccgtccgg gcgtgctaac tgcgctgaac
     9781 aaaatcttcg ccgagcaggg cgtcaacatc gccgcgcaat atctacaaac ttccgcccag
     9841 atgggttatg tagttattga tattgaagct gacgaagacg ttgccgaaaa agcgctgcag
     9901 gcaatgaaag ctattccggg taccattcgc gcccgtctgc tgtactaatt ccccttctct
     9961 gaaaattaac gggcaggtca ctgacttgcc cgttttttta tcccttctcc acaccgttgc
    10021 cgcgttgcgc attctacttt gcgagtctgc tccagaaaat aaatcaaagt cagatcaatg
    10081 cgttgtgttt atttaatggg gatttacgat ggcgggacag tatggggctg taacggtaaa
    10141 gccctcaccg aagcgagggc ttaaaggaga agggttatga tgcgacttgt catcatactg
    10201 attgtactgt tactgataag tttcggagct tattaacagt cagtctctgg ggaggagtca
    10261 tcctccctta cccttactca ctaaattagg tcaaagaatc aacgatgtca atcagggcga
    10321 tgcggggtgc atcgccctga accgaacttt ttgatagcca cttcatcgcc cacgtttact
    10381 gccgtaatag atcctttttc ccaaaaatta tctctgtttt gcatggggtt tttcgaacac
    10441 gctcgaagaa aagatttaaa ggtagatcaa tgcgttgtgg ttatttaaag agagcttacg
    10501 atagctgggc agcatggggc tgtaacggta aagccctcac cgaagcgagg gcttgaagga
    10561 gaagggttat gatgcgactt gccatcatac tgattgtact gttactcata agtttcccag
    10621 cttattaaca gtcagtcaca ggggaggagc aatcctccct tacccttact cactaaatta
    10681 ggttaaagaa tcaacgatgt caatcagggc gatgcaggtg tatcgccctt accactccca
    10741 gactttcgac ggtgtaacca ccgcaggaag agggatatcc cactcttcaa cggggagttt
    10801 ttccaccaac tgacaatcat gcgcataacc caccggttgc gttttatagt gctgccagtt
    10861 ttgtaaggtc cgatcataaa aaccaccgcc cattcccagg cgctgaccgt actcatcaaa
    10921 ggcgaccagc ggtgtgatca gcacgtctaa tcgggaaagg ggtagcacgt cacgcacatc
    10981 cagttttagc tcatggattt tcaacctgtt catcaccagt tcgctttgcg gatggtaatt
    11041 caggaacagc aaattaccag cactaaaggg atgcaaaact ggaagatata cgcgcttacc
    11101 ggcgcgccag agttgttcta ttagcggctg ggtgtcgagt tcaccatcaa aagagaggaa
    11161 tacagcgacc gtatgcgcca tcaccaccgg gggataagtc atcatccggg tagcggcttg
    11221 ttgacccatt tcctgctgtt gttccggcgt taacgcacga cgacgttgcc gaatcatttt
    11281 gcggatttct tgtcgggata atgtcagtgg gagttctggt agttgcgtca tggctggtgc
    11341 cagataagaa gggaatctcc gagatgccgc cgcaggctgt aacccttgaa cccttggttc
    11401 aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg gaccgagcat gctcaccaac
    11461 cgcggagcgc cacattcttg tggtatgaaa tatcggctca ggggactggc ccgcttgcga
    11521 acatctcaga gaaattttgt cttcacggtt actctaccac agtaaaccga aaagtgtcat
    11581 tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt caagtaacgc ttgttctatg
    11641 gtctgctgca gcatccgaat acgctgttcc atacttgccg cgtagtcacg agtcttcgct
    11701 ttttcttgcg ctaactcata gctgatattc aatgcggcaa tgaagaccaa ctgttcagta
    11761 tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt ggttcagatc gtccgctgcc
    11821 tgattcaacg catccctttg gtcaggcggg cagttcacac gcagtgaacg gccaaaaatt
    11881 tggatatcga cgggttgtgc agacatgcca ccttcctgct gattgactgc gctgccttcg
    11941 tctttagacc catggtctgc gaaggggcga cactatagct accctgatga gaagagacaa
    12001 gcccttttct ggtccaccag ggcccaaagt ggtagcatat catgaatatt cctccctttg
    12061 acgacgaatg cttatgtcta tacagaacga aatgcctggt tacaacgaaa tgaaccagta
    12121 tctgaaccaa caagggacgg gtctgacccc tgccgagatg catggtttaa tcagtgggat
    12181 gatatgtggc ggtaacgatg acagctcatg gctgccgcta cttcacgacc tgacgaacga
    12241 aggcatggct ttcggtcatg agctggcaca ggcactgcgt aaaatgcact ctgccaccag
    12301 cgatgccctg caggatgacg gcttcctttt tcagctttat ctgcctgatg gcgatgatgt
    12361 cagcgttttc gatcgggctg atgcattggc aggttgggtc aatcacttcc tgcttggtct
    12421 tggcgttacg caaccgaagc tggataaagt gaccggcgaa accggtgaag ctatcgacga
    12481 tctgcgtaac attgcgcaac tgggttacga cgaagacgaa gatcaggaag agcttgaaat
    12541 gtcgcttgaa gagatcatcg aatacgttcg tgttgccgcg ctgttatgcc acgacacctt
    12601 tactcatccg caaccgaccg cgccagaagt acaaaaaccg actctacact aaaaacaaaa
    12661 aacgtaagga gagtgttatg agtgagatat cccggcaaga gtttcagcgt cgccgtcagg
    12721 ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat ttttgctgca ccagaagtaa
    12781 cacgtagcgc cgacagcgaa tacccctatc gtcagaacag tgacttctgg tacttcaccg
    12841 gctttaacga accggaagcg gtgctggtgc tgattaaaag cgatgacact cataaccaca
    12901 gcgttctgtt taaccgcgtt cgcgacctga cggcggagat ctggtttggc cgtcgcttag
    12961 gccaggatgc cgcgccagag aaactgggcg ttgaccgcgc actggcattc agcgaaatca
    13021 atcagcaact ttatcaacta cttaacggcc tggatgtggt ttaccatgcc cagggcgaat
    13081 atgcatatgc tgatgaaatc atgaacagtg cgctggaaaa actgcgtaaa ggttcgcggc
    13141 aaaatctcac cgcaccggca acgatgatcg actggcgtcc tgttgttcat gaaatgcgcc
    13201 tgttcaaatc gccagaagag attgccgtac tccgccgcgc gggagaaatc accgccatgg
    13261 cacatacacg ggcgatggaa aaatgccgtc cgggaatgtt cgagtaccat ctggaaggcg
    13321 aaattcacca cgaatttaac cgccacggtg cgcgctatcc gtcctataac accattgtcg
    13381 gcagcggtga aaacggctgc attctgcact acaccgaaaa cgagagtgaa ctgcgcgacg
    13441 gtgacctggt gttgattgac gcgggttgtg aatacaaagg ttacgcgggc gatattaccc
    13501 gcaccttccc ggtcaacggc aaattcaccc aggcccagcg tgaaatctac gacattgtgc
    13561 tggagtctct cgaaaccagc ctgcgcctgt atcgtccggg aacttccatt ctggaagtca
    13621 ctggtgaagt ggtgcgcatc atggttagcg gcctggtaaa actcggcatt ctgaaaggtg
    13681 atgttgatga actgatcgct cagaatgccc atcgtccttt ctttatgcat ggccttagcc
    13741 actggttagg gctagatgtc catgacgttg gcgtttatgg tcaggatcgc tcgcgcattc
    13801 tggaaccggg catggtactg accgtagagc cagggctgta tattgcgccg gatgcagaag
    13861 tgccagaaca atatcgcggt atcggcattc gtattgaaga cgacattgtg attaccgaaa
    13921 ccggtaacga aaacctcacc gccagcgtgg tgaaaaagcc ggaagaaatc gaagcgttga
    13981 tggctgctgc gagaaagcaa tgagcgtaat catcgtcggt ggcggcatgg cgggcgcgac
    14041 gctggcgctg gctatttccc ggttaagtca cggggcgctg ccggtacatt tgattgaagc
    14101 gactgcgcca gagtcacatg ctcatccggg ctttgatgga cgagcgatag cgctggcggc
    14161 gggtacctgt cagcaactgg cgcgcatcgg cgtctggcaa tctctggcgg attgcgcaac
    14221 tgccatcacc accgtgcatg tcagcgatcg tggtcacgct ggatttgtca ccctcgccgc
    14281 agaagattac caactggcgg cgctgggaca ggttgtcgaa ttgcacaatg tcgggcaacg
    14341 gctgtttgca ttgctgcgta aagcacctgg cgtaacgctg cattgccctg atcgcgtggc
    14401 taacgttgcc cgtactcaga gtcacgttga agtgacgctg gagagtggcg agacgctgac
    14461 gggccgcgtg ctggtagcag ctgatggcac ccattcagcg ttagccaccg cgtgcggcgt
    14521 tgactggcag caggagcctt acgaacaact ggccgtgatt gccaacgttg cgacttccgt
    14581 tgcgcacgaa ggccgcgctt ttgaacgctt cacgcaacat ggcccgctgg cgatgttgcc
    14641 gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca ctggaacggc gcgaagaggt
    14701 gctgtcgtgg agcgacgaga agttttgccg tgaactccag tcggcctttg gctggcgact
    14761 tggaaaaatt acccacgccg gtaaacgcag tgcttatccg ctggcgttaa cccgcgccgc
    14821 cagagctatt acccatcgta ccgtgctggt gggcaatgcg gcgcaaactc tgcacccgat
    14881 tgccgggcaa gggtttaacc tcggtatgcg agatgtcatg agccttgcgg aaaccctgac
    14941 tcaggcgcat gagcgcggag aagacatcgg tgattacggc atattgtgcc gctatcagca
    15001 gcgtcgacag agcgatcgcg aagcaaccat cggcgtcacg gacagcctgg tacatctttt
    15061 tgccaaccgt tgggcaccgc tggttgtcgg gcgcaacatc ggactgatga cgatggaatt
    15121 attcaccccg gcacgcgatg tgctggcgca gcgcaccctc ggttgggtgg cgcgttgaag
    15181 gcggttttaa ggaataaaca atgcaatgtg ttgatgtagc cattgttggt ggcggcatgg
    15241 tggggctggc ggttgcctgc ggcttacagg ggagcggctt acgcgttgcc gtactggagc
    15301 agcgcgtacc ggaacctctg gcggcggatg caccaccaca actgcgcgtt tcggctatca
    15361 atgccgccag cgaaaaatta ctcacccgtc ttggcgtctg gcaggacatt ctctctcgta
    15421 gggccagttg ttatcacggt atggaagtgt gggacaaaga cagttttggt cacatttcgt
    15481 ttgacgatca aagcatgggc tatagccatc ttgggcatat cgttgaaaac tcagtgattc
    15541 actacgcgct gtggaacaaa gcgcagcagt cgtcagatat cactctgttg gcccccgcag
    15601 aattacagca ggtcgcctgg ggagaaaatg aaactttcct gacgctgaaa gatggcagca
    15661 tgttaacggc gcgtctggtg attggcgcgg acggtgctaa ttcctggttg cgcaacaaag
    15721 cagatatttc gctgactttc tgggattatc agcatcacgc gctggtagcg accattcgca
    15781 cggaagaacc gcatgatgcg gtggcgcggc aggttttcca tggcgaaggc attctggcct
    15841 ttttaccgct tagcgatccg catctttgct caattgtctg gtcactgtcg ccagaggaag
    15901 cgcagcggat gcagcaggca agtgaagatg aatttaatcg cgcgttaaat atcgcttttg
    15961 ataatcgcct gggcttatgc aaggttgaga gcgcgcgtca ggtgttccca ctgacggggc
    16021 gttatgcgcg ccagtttgcc gcgcaccgtc tggtgctagt gggcgacgcc gcgcatacca
    16081 ttcacccgct ggcggggcag ggagtaaatc tcggctttat ggatgctgca gagctgattg
    16141 ctgaactgaa acggttgcat cgtcagggga aagacatcgg gcagtacatt tacctgcgcc
    16201 ggtatgagcg tagccgcaag cacagtgcgg cgctgatgct ggctggtatg cagggattcc
    16261 gcgatctgtt ttccggtacc aatccggcga aaaaactgct gcgtgatatt ggtttgaaac
    16321 tggccgatac gcttcctggc gttaaaccac aacttatccg ccaggcaatg ggattaaacg
    16381 atttgcctga atggctgcgt taaaaatttc tcttctgctg tttatttgat acccatcaca
    16441 ctttcatctc ccggtttttt cgccgggaga ttttcctcat ttgaaataaa ctaatttcac
    16501 ctccgttttc gcattatatt ttctaatgtc attatttttt gatttagtgt tttttgacat
    16561 ttttttagct cttaatattg ccttattcaa attagatttc taatggcatc atctttatat
    16621 agaaactggt gtgttttttg gttttttatt ctgtcgcgat tttggcgttt tttaaccata
    16681 agctaatgtg atgatcaatt ttaccttatg gttaacagtc tgtttcggtg gtaagttcag
    16741 gcaaaagaga acgattgcgt tggggaccgg gagtggctcc gatgctgggt ttcgtggtga
    16801 taatttcacc atgaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    16861 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    16921 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    16981 cggaatgttt gatgtgtcac atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    17041 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    17101 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gacctcatcg tctactactt
    17161 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    17221 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    17281 gattgccgtg caagggccga atgcgcaggc aaaagctgcc acactgttta atgacgccca
    17341 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggcgggcg atctgtttat
    17401 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    17461 ggccgatttc tggcgtgcgc tggtggaagc gggtgttaag ccatgtggct tgggcgcgcg
    17521 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    17581 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccggcag atcgtgactt
    17641 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    17701 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    17761 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    17821 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    17881 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    17941 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    18001 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    18061 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    18121 gttagcgcgg gcgatgactg cgcggttgcc gaatcagtaa aagcggcgtc agacatttat
    18181 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc tccggaactg
    18241 gtgaacagcg aaccgtatgc aggtggctgg atctttaaaa tcaaagccag cgatgaaagc
    18301 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga cgagtaacgg
    18361 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    18421 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    18481 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    18541 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggcgca caatcgttaa acgcgctgac
    18601 cggccagatt gtaccgaaag atattcagct tgcgaccccg ccgcaggttg gcgcaccggc
    18661 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    18721 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    18781 gctggaaaat ccgggctggt ataccgcgta cactccgtat caacctgaag tctcccaggg
    18841 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    18901 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    18961 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccgatg tgcatccgca
    19021 aacgctggat gtggtccgta ctcgtgccga aacctttggt tttgaagtga ttgtcgatga
    19081 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    19141 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    19201 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    19261 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    19321 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    19381 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    19441 gcaacatatc cgccgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    19501 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    19561 ccgcattcac cgtttgaccg atatcctggc ggcaggcctg caacaaaaag gtctgaagct
    19621 gcgccatgcg cactatttcg acactctgtg tgtggaagtg gccgacaagg cgggcgtact
    19681 ggcgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg cggttgggat
    19741 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagcg tgctgctggg
    19801 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    19861 tattcagcct gcgatgctgc gcgacgacga aatcctcact catccggtgt ttaatcgcta
    19921 ccacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    19981 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    20041 gatcccaatc acctggccgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    20101 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    20161 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    20221 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    20281 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    20341 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    20401 gggcgataac ctctcctgta tcatggtgac ttatccctct acccacggcg tgtatgaaga
    20461 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    20521 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    20581 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    20641 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    20701 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    20761 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    20821 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    20881 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    20941 gaaagaagaa accagcatca gcgagctgga tattgccaag cgcctgatcg actacggttt
    21001 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    21061 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    21121 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    21181 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    21241 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    21301 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa tacctgtaat tcactgattc
    21361 gactattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    21421 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    21481 gcgcgaatat ttaccctcac cccggccctc tccctgaaag ggcgagggga aaagcgtgcc
    21541 aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg aattaatttt
    21601 tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc atcgggcggg
    21661 caactgcatt actgttggcg aaagaagagt atacggtggc ggttaattat caacaaaacc
    21721 tccatgcggc gcaggaagtg gtgaacttaa tcacgcaagc cggtggcaag gcattcgtgc
    21781 tccaggcgga tatcagcgac gaaaatcagg tcatcgcaat gtttacagca atcgatcagc
    21841 acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc cagtgcaccg
    21901 ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg acgggatatt
    21961 ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc agtggcggcg
    22021 ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa taggttgatt
    22081 acgcggcatc gaaaggggcg attgatacgt taaccaccgg actatcgctg gaagtcgccg
    22141 cgcaggggat ccgcgttaac tgcgtgcggc cagggtttat ttataccgaa atgcacgcca
    22201 gcggcggcga gcttggacgc gtcgatcgcg ttaagtcgaa catccccatg cagcgtggtg
    22261 gacaggcaga agaggtcgcg caggccattg tctggctact aagtgataaa gcctcttacg
    22321 tcacgggaag ttttatcaat ttggcgggcg ggaaataaaa cagggaagtt gtctgaccgg
    22381 atgcaacaag tattgcatcc ggtacttcat cgacttaaag cttctcgccg ttgctggcaa
    22441 tcacctcttt gtaccagtta aagctcttct tacgtgaacg cgacatatcg ccagtaccgt
    22501 cgtcatgttt attcacatag ataaagccgt agcgtttgct gtactgcccg gtagtgaatg
    22561 acacgcagtc gatgcagccc cacggtgtgt agcccatcag atccacacca tcgtaagtca
    22621 ctgctttttt catctcttca atatgggcgc gcaggtagtc aatgcggtag tcgtcgttga
    22681 tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc gttttcgaca ataaacagcg
    22741 gcctctgata acgctcatac agttcgcaaa gggcatagcg cagacctact ggatcaatct
    22801 gccagcccca gtcagacgct ttaacatacg ggttcggtac gctgccttca aaaccagaga
    22861 tcgcatcgcc ggtgccgcct tcggccttca ctgcgttgat catgtaatag ctgaaaccaa
    22921 gataatcgca ggtgccttca cgcagtacat ccagatcgcc atcttccatt ttgatgttaa
    22981 atccgcgacg ctcccactcg ttcaacacat aggacgggta atagccgcgt agctgcacat
    23041 cggtaaagac gtagcgttcg cgcatcgact cctgagcgaa catcacatcg tccgggttac
    23101 aggagtaagg atagagcggc accatcgcca gcatacagcc gactttcatc tccgggttaa
    23161 tgcgacgcgc ggctttcact gctagggcgc tggcgacaaa ctggtgatgc agcacctggt
    23221 acatcgtctc ttccgggttg tcatgctcgg tatacaccac gccggagcag cagtaaccaa
    23281 acagcggcgc acgccagtta cgctggttgt ttatttcgtt gaaggtcatc cagtatttga
    23341 ctttatgctt gtagcgttcg aataccactt ccgcgaaacg gacaaagaaa tcaacgactt
    23401 tacggttggt ccagctaccg tattgctgca ccagatgcag cggtatttca aagtgggaga
    23461 gggttatcac cggttcgatg ttgtatttca gcagttcatc gaacagagag tcgtaaaact
    23521 tcagcccttc ttcgtttggc tgagcttcat caccttttgg aaaaatacgc gtccaggcga
    23581 tggaagtccg aaaacatttg aagcccattt cggcaaatag cttgatgtct tccttgtagt
    23641 gaccataaaa atcaacggct tcatggtttg gatagtattt tcctggcaag acttctttgg
    23701 tgatttcgcg cggcacgccg tgtgcgccac cggtcagaac gtcacaaatg ctcggtcctt
    23761 tgccgccttt gttccagccg ccttcgacct gatgagcggc aacggcgcca ccccataaga
    23821 aatctttcgg taaggtgagt tttttcacta tcataggctc cagatgagtc aattcttaca
    23881 ggaaatagta ccatgcagcc aaacgacatc actttttctc aacgtttcca ggatgacatt
    23941 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    24001 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    24061 gcaacctcaa ccgtaacgct ggatacgctg acggaaaaac atgcagagca ggaaaatatg
    24121 accctgactg aactgataaa ggtcattgcc gacatctatc ccggtcagac acagttttat
    24181 gtgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttctcattt
    24241 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    24301 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctccgga
    24361 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    24421 caacagcgtc agtcacggca ttgggttggt gtttggtatc gttgagctgg tgttgctact
    24481 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagctaca gcctctatgg
    24541 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    24601 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    24661 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    24721 tatctggagc ctggcattgc tggggattct gtttaaactg accatcgcgc accgattcaa
    24781 aattttatct ctggtgacct atctggcgat gggctggctg tcgctggtgg taatttatga
    24841 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    24901 ttcgctcgga gtgattttct atgtctgcaa acgcattccc tacaatcatg ccatctggca
    24961 cggcttcgtg ctcggcggca gtatgtgcca ctttctggcg atctatttgt atattgggca
    25021 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg
    25081 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    25141 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    25201 atgcctgatg cgatacagag atttactctt cgagtgaata cggcagcggc tcgatatgca
    25261 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    25321 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    25381 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    25441 cgctacctgc cagcaaccag agcgcacgtt tattggcacc acggaatttt gctcgcgcca
    25501 ccatctcttg tccggtataa cagcctttct taaaactgat accgcccagc gcctggagat
    25561 tggtcgcctg tgggataaac tgcccgctgt tcgcggcatc aatcaccggg aaacccgctt
    25621 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    25681 cggtcagcat attggcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    25741 caaaccatag caaagtggtc gcgccttctt taactacctg tttttctctc gaaggtagtt
    25801 cactaaagag attggcgagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    25861 gctcgtcgtc tgccgcgatg gtcactttag agaacaccgc atattttttc agttcagtca
    25921 gctgcggttc acgcacgctg cgccgttcaa tccacgcaaa gccatcgccg tcgcggaaca
    25981 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    26041 tttctgtcat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    26101 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    26161 cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    26221 cttaacggcg tattaataac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    26281 tacgcgatct gtgccttctg cgaggcgact tccaaaaaaa tgaagctctt gaattgttgt
    26341 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    26401 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    26461 aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    26521 ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    26581 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    26641 gatgcagaac tggaaatgat ggtccgactc atccagacac ggaaccggga acgtggtcct
    26701 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    26761 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    26821 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggcgta ttaatgctcg
    26881 ccagggggaa attcgattgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    26941 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    27001 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagctg aatggcggga
    27061 tttacggcgg attttgttgc aacaagagac gcaaagataa atatgacggc aaagcatatg
    27121 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    27181 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    27241 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    27301 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    27361 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaacaa
    27421 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    27481 tttcgccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    27541 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    27601 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    27661 taacgtaagc ttctcgattc tgctgtggct tttgcggcta gtgtatcaga atcgctttag
    27721 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    27781 ggatctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    27841 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    27901 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    27961 agagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    28021 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    28081 gaaattgcca cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    28141 ggcaccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    28201 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctacg
    28261 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttaggtga
    28321 agaggccgtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgttgaatgg
    28381 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    28441 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    28501 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    28561 ggtgcagatg ctactgggcc acagcgatct ctccaccacg caaatttata cgcatgtcgc
    28621 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    28681 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    28741 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcattaaaag
    28801 cagcgatatt cagccagcac ccgtggctgg catgaagact gtgctgacta acagcggtgt
    28861 gttgtacatc accgatgacg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    28921 cgcggctccg gtcaatgtca ccaataaaat gctgctaaag cagttgaatg cgcttgaaaa
    28981 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    29041 tacctgtggg tactgccaca aactgcatga gcaaatggca gactacaacg cgctggggat
    29101 caccgtgcgt tatcttgctt tcccgcgcca ggggctggag agccaggctg agcaacaaat
    29161 gaaagccatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggctggtaa
    29221 agctgccact ccggcaagtt gcgatatcga tattgccgat cattacgcac ttggcgtcca
    29281 gcttggcgtt agcggcactc cggcaattgt gctgagcaat ggcacacttg ttccgggtta
    29341 ccagccgccg aaagagatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    29401 ttaattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    29461 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    29521 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    29581 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    29641 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    29701 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    29761 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    29821 aacggtattt cctcccatgc gggggttgaa cacgctcgct cgttgggcat tccggttatt
    29881 gttaccgatc accatttgcc aggcgaaaca ttacccgcgg cggaagcgat cattaaccct
    29941 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    30001 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    30061 gcaattccta acctggcaga actgctggat ctggtcgcgc tggggacagt ggcggacgtc
    30121 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    30181 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaaccgtga tgcacaaaaa
    30241 ctcgccgcca gcgatttagg ttttgcgctg gggccacgtc tcaatgctgc cgggcgactg
    30301 gacgatatgt ccgtcggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    30361 ctggcaaatg aactcgatgc gctaaatcag acgcgaaaag agatcgaaca aggaatgcag
    30421 attgaagcct tgaccctgtg cgagaaactg gagcgaagtc gcgacacgct acccggcggg
    30481 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgcatc
    30541 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgatgg tacgctgaaa
    30601 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    30661 ctctaccccg gtatgatgct gaagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    30721 gaagaggata aattcgaact ctttcaacaa cggtttggcg agctggttac cgagtggctg
    30781 gacccttcgc tattgcaagg cgaagtggtg tcagacggcc cgttaagccc ggccgaaatg
    30841 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    30901 ccgctgtttg atggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    30961 aaagtcatgg tcgaaccggt cggcggcggt ccgctgctgg atggtattgc ttttaatgtc
    31021 gataccgccc tctggccgga taacggcgtg cgcgaagtgc aactggctta caagctcgat
    31081 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    31141 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    31201 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    31261 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    31321 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    31381 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    31441 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    31501 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    31561 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgcta
    31621 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    31681 gcgtatgtat ttgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    31741 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    31801 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaagagcc cgtttgactc
    31861 cggcggtcgt cgccacacgt cgttcagctc cgcgtttgtt tatccggaag ttgatgatga
    31921 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    31981 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    32041 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aataaagacc aggccatgaa
    32101 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgctg agaaacaggc
    32161 gatggaagat aacaaatccg acatcggctg gggcagccag attcgttctt atgtccttga
    32221 tgactcccgc attaaagatc tgcgcaccgg ggttgaaacc cgcaatacgc aggccgtgct
    32281 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    32341 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    32401 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    32461 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    32521 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    32581 gggtaaagcc tcttttgtta ccctgcagga cgttggcggt cgtattcagc tgtacgttgc
    32641 ccgtgacgat cttccggaag gcgtttacaa cgagcagttc aaaaaatggg atctcggcga
    32701 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    32761 caccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggacaaat tccacggctt
    32821 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    32881 ccgcaacacc tttaaagtgc gttcgcagat cctctctggc attcgccagt tcatggtaaa
    32941 tcgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    33001 gcgtccgttt atcacccatc ataacgcgct ggatcttgac atgtacctgc gtatcgcgcc
    33061 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    33121 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    33181 ctatatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    33241 tctggcacag gatattctcg gtaagacgga agtcacctac ggcgacgtga cgctggactt
    33301 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    33361 cgacatggcg gatctggaca acttcgactc tgcaaaagcg attgctgaat ctatcggcat
    33421 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    33481 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    33541 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    33601 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcagcgctt
    33661 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgacgaaga
    33721 ttacgtcacc gcactggaac acggcttacc gccgacagca ggtctgggaa ttggtatcga
    33781 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    33841 gatgcgtcca gtaaaataag cattacgtta tgctaataac cccggcaaat gtcggggttt
    33901 tttaattgtg ctgcgcgaaa gcagacaaca attttcttgc ttcactattg gctgcctgca
    33961 ttaccatcca cggactgaac gcccacggcg tggcatcaat accgtgtaat acatctgcta
    34021 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctgttggtgc
    34081 gtgcggcaaa taccggacac acttcatttt ccacaatgcc attcggatcg gtggcgcgat
    34141 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    34201 aacggcggat cactgcgtct tcgttgcttt ctcccagttg tgggtgccca caaaccgagt
    34261 tagtccacac gccaggccat gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    34321 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    34381 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    34441 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    34501 tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    34561 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    34621 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacagcagac tgaatatagc
    34681 gcgcattaaa agatttatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    34741 gccacatacg tactgtcctg acttttaaat ctttatcaga gacggattcc ttcaccaggt
    34801 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    34861 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    34921 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    34981 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    35041 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    35101 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    35161 tatgaacgcg cgtcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    35221 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg cgaataatat
    35281 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    35341 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    35401 gcataccaaa cgacatcggg gtgacaatcg caaaccacaa ggcatcatgt agcccggata
    35461 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    35521 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    35581 caaaggagat acctgaataa acgggattac catattgcgg attcccttta cctccggcgg
    35641 cccagtcaat acccacctga atgatgctaa gtccgacaga agtaataacc acaccggtaa
    35701 ccagtggcgg gaataaaggc atcaagcgac cgataagtgg cgctaataat gtggtgataa
    35761 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc aatatccggg ttcatcccaa
    35821 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    35881 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    35941 ccgagctaat gagcatcgca atagcttctt ttgagaggcc cagtcggtca ccaatcataa
    36001 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    36061 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    36121 agagaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagagtaaaa
    36181 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    36241 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg gtt
//
LOCUS       DAJTOR010000018        36119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTOR010000018 DAJTOR010000000
VERSION     DAJTOR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.018"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            269..355
                     /gene="leuL"
                     /locus_tag="OO249_000828"
     CDS             269..355
                     /gene="leuL"
                     /locus_tag="OO249_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5635218.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            448..2019
                     /gene="leuA"
                     /locus_tag="OO249_000829"
     CDS             448..2019
                     /gene="leuA"
                     /locus_tag="OO249_000829"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5635219.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2019..3110
                     /gene="leuB"
                     /locus_tag="OO249_000830"
     CDS             2019..3110
                     /gene="leuB"
                     /locus_tag="OO249_000830"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5635220.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3113..4513
                     /gene="leuC"
                     /locus_tag="OO249_000831"
     CDS             3113..4513
                     /gene="leuC"
                     /locus_tag="OO249_000831"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5635221.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGQVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4524..5129
                     /gene="leuD"
                     /locus_tag="OO249_000832"
     CDS             4524..5129
                     /gene="leuD"
                     /locus_tag="OO249_000832"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5635222.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5178..6260)
                     /locus_tag="OO249_000833"
     CDS             complement(5178..6260)
                     /locus_tag="OO249_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5635223.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYEDNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(6361..6492)
                     /gene="sgrT"
                     /locus_tag="OO249_000834"
     CDS             complement(6361..6492)
                     /gene="sgrT"
                     /locus_tag="OO249_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5635224.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6581..8236
                     /gene="sgrR"
                     /locus_tag="OO249_000835"
     CDS             6581..8236
                     /gene="sgrR"
                     /locus_tag="OO249_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5635225.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSTLRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVNYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8270..8369
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8400..9383
                     /gene="thiB"
                     /locus_tag="OO249_000836"
     CDS             8400..9383
                     /gene="thiB"
                     /locus_tag="OO249_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5635226.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            9359..10969
                     /gene="thiP"
                     /locus_tag="OO249_000837"
     CDS             9359..10969
                     /gene="thiP"
                     /locus_tag="OO249_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5635227.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            10953..11651
                     /gene="thiQ"
                     /locus_tag="OO249_000838"
     CDS             10953..11651
                     /gene="thiQ"
                     /locus_tag="OO249_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5635228.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11764..12528)
                     /locus_tag="OO249_000839"
     CDS             complement(11764..12528)
                     /locus_tag="OO249_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5635229.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12614..13492)
                     /gene="araC"
                     /locus_tag="OO249_000840"
     CDS             complement(12614..13492)
                     /gene="araC"
                     /locus_tag="OO249_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5635230.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13831..15531
                     /gene="araB"
                     /locus_tag="OO249_000841"
     CDS             13831..15531
                     /gene="araB"
                     /locus_tag="OO249_000841"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5635231.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQTQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15542..17044
                     /gene="araA"
                     /locus_tag="OO249_000842"
     CDS             15542..17044
                     /gene="araA"
                     /locus_tag="OO249_000842"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5635232.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQHLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17244..17939
                     /gene="araD"
                     /locus_tag="OO249_000843"
     CDS             17244..17939
                     /gene="araD"
                     /locus_tag="OO249_000843"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5635233.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18014..20365
                     /gene="polB"
                     /locus_tag="OO249_000844"
     CDS             18014..20365
                     /gene="polB"
                     /locus_tag="OO249_000844"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5635234.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGSENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20529..23435
                     /gene="rapA"
                     /locus_tag="OO249_000845"
     CDS             20529..23435
                     /gene="rapA"
                     /locus_tag="OO249_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5635235.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHMPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23447..24106
                     /gene="rluA"
                     /locus_tag="OO249_000846"
     CDS             23447..24106
                     /gene="rluA"
                     /locus_tag="OO249_000846"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5635236.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24223..25038)
                     /gene="djlA"
                     /locus_tag="OO249_000847"
     CDS             complement(24223..25038)
                     /gene="djlA"
                     /locus_tag="OO249_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5635237.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25293..27647
                     /gene="lptD"
                     /locus_tag="OO249_000848"
     CDS             25293..27647
                     /gene="lptD"
                     /locus_tag="OO249_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5635238.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27700..28986
                     /gene="surA"
                     /locus_tag="OO249_000849"
     CDS             27700..28986
                     /gene="surA"
                     /locus_tag="OO249_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5635239.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            28986..29975
                     /gene="pdxA"
                     /locus_tag="OO249_000850"
     CDS             28986..29975
                     /gene="pdxA"
                     /locus_tag="OO249_000850"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5635240.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMILNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            29972..30793
                     /gene="rsmA"
                     /locus_tag="OO249_000851"
     CDS             29972..30793
                     /gene="rsmA"
                     /locus_tag="OO249_000851"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5635241.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30796..31173
                     /gene="apaG"
                     /locus_tag="OO249_000852"
     CDS             30796..31173
                     /gene="apaG"
                     /locus_tag="OO249_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5635242.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31180..32022
                     /gene="apaH"
                     /locus_tag="OO249_000853"
     CDS             31180..32022
                     /gene="apaH"
                     /locus_tag="OO249_000853"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5635243.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32100..32579)
                     /gene="folA"
                     /locus_tag="OO249_000854"
     CDS             complement(32100..32579)
                     /gene="folA"
                     /locus_tag="OO249_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5635244.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32771..34633)
                     /gene="kefC"
                     /locus_tag="OO249_000855"
     CDS             complement(32771..34633)
                     /gene="kefC"
                     /locus_tag="OO249_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5635245.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34626..35156)
                     /gene="kefF"
                     /locus_tag="OO249_000856"
     CDS             complement(34626..35156)
                     /gene="kefF"
                     /locus_tag="OO249_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5635246.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35264..36119)
                     /locus_tag="OO249_000857"
                     /pseudo
     CDS             complement(35264..36119)
                     /locus_tag="OO249_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8710 a         9925 c         9551 g         7933 t
ORIGIN      
        1 tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt tcctcgttga ttagatgcaa
       61 aaatttattc tgaaatatgt caacctatga aaagtgtcgg tcgttaaaca gaaattaata
      121 tcgcttgctt taaccaccgc agcacaatta gctaatttta cggatgcaga actcgcgctg
      181 gcgggacgtt tttattgcgt cagggttgac atccgttttt gtatccagta actctaaaag
      241 catatcgcat tcatctggag ctgatttaat gactcacatc gttcgcttta tcggtctact
      301 actactaaac gcatcttctt tgcgcggtag acgagtgagc ggcatccagc attaagccag
      361 cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt ttttatgccc gaagcgaggc
      421 gctctaaaag agacaaggac ccaaaccatg agccagcaag tcattatttt cgataccaca
      481 ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg tgaaagaaaa actgcaaatt
      541 gcgctggccc ttgagcgtat gggtgttgac gtgatggaag tcggtttccc cgtctcttcg
      601 ccgggcgatt ttgaatcagt gcaaaccatc gcccgccagg ttaaaaacag ccgcgtatgc
      661 gcgttagctc gctgcgtgga gaaagatatc gacgtggcgg ccgaatccct gaaagtcgcc
      721 gaagccttcc gtattcatac ctttattgcc acttcgccaa tgcacatcgc caccaagctg
      781 cgcagcacgc tggatgaagt gatcgaacgc gctatctata tggtgaaacg cgcccgtaat
      841 tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc gtacccccat tgccgatctg
      901 gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca ccatcaacat tccggacacc
      961 gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca gcggtctgta tgaacgcgta
     1021 cctaacatcg acaaagccat tatttccgta catacccacg acgatttagg cctggcggtc
     1081 ggcaactcac tggcggcggt acatgccggt gcacgccagg tggaaggtgc aatgaacggg
     1141 atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca tcatggcgat taaagttcgt
     1201 aaggatattc tcaacgtcca caccgccatt aatcaccagg agatatggcg caccagccag
     1261 ttagttagcc agatttgtaa tatgccgatc ccggcaaaca aagccattgt tggcagcggc
     1321 gcattcgccc actcctccgg tatccaccag gatggcgtgc tgaaaaaccg cgaaaactac
     1381 gaaatcatga caccagaatc tattggtctg aaccaaatcc agctgaatct gacctctcgt
     1441 tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg ggtataaaga aagtgaatat
     1501 aatttagaca atttgtacga tgctttcctg aagctggcgg acaaaaaagg tcaggtgttt
     1561 gattacgatc tggaggcgct ggccttcatc ggtaagcagc aagaagagcc ggagcatttc
     1621 cgtctggatt acttcagcgt gcagtctggc tctaacgata tcgccaccgc cgccgtcaaa
     1681 ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg gtaacggtcc ggtcgatgcc
     1741 gtctatcagg caattaaccg catcactgac tataacgtcg aactggtgaa atacagcctg
     1801 accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg atattgtcgc caactacaac
     1861 ggtcgccgct tccacggcgt cggcctggcc accgatattg tcgagtcctc cgccaaagcc
     1921 atggtgcacg tactgaacaa tatctggcgt gccgcagaag tcgaaaaaga gttgcaacgc
     1981 aaagctcaac acaacgaaaa caacaaggaa accgtgtgat gtcgaagaat taccatattg
     2041 ccgtattgcc gggggacggt attggtccgg aagtgatgac ccaggcgctg aaagtgctgg
     2101 atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag ccattacgat gtaggcggcg
     2161 cagccattga taaccacggg caaccactgc cgcctgcgac ggttgaaggt tgtgagcaag
     2221 ccgatgccgt gctgtttggc tcggtaggcg gtccgaaatg ggaacattta ccaccagacc
     2281 agcaaccaga acgcggcgcg ctgttgcctt tgcgtaagca cttcaaatta ttcagcaacc
     2341 tgcgtccggc aaaactgtat caggggctgg aagcattctg tccgctgcgt gctgacattg
     2401 ccgctaacgg cttcgacatc ctgtgcgtgc gcgaactgac cggcggcatc tatttcggtc
     2461 agccaaaagg ccgcgaaggt agcggacagt atgaaaaagc gtttgatacc gaggtgtatc
     2521 accgttttga gatcgaacgt atcgcccgca tcgcgtttga atctgcccgc aagcgtcgcc
     2581 acaaagtcac ctcaatcgac aaagccaacg tgctgcaatc ctctatttta tggcgggaga
     2641 tcgttaacga gatcgccacg gaatacccgg atgtcgaact ggcgcatatg tacatcgaca
     2701 acgccaccat gcagctgatt aaagatccat cacagtttga cgtcctgctg tgctccaacc
     2761 tgtttggcga cattctgtct gacgagtgcg caatgatcac tggctcgatg gggatgttgc
     2821 cttccgccag cctgaacgag caaggttttg gtctgtatga accggcaggc ggctcagcac
     2881 cagatatcac aggcaaaaac atcgccaacc cgattgcgca aattctgtcg ctggcactgc
     2941 tgctgcgcta cagcctggat gccgatgatg cggcttgcgc cattgaacgc gccattaacc
     3001 gcgcattaga agaaggcatt cgcaccgggg atttagcccg tggcgctgcc gccgttagta
     3061 ccgatgaaat gggcgatatc attgcccgct atgtggcaga aggggtgtaa tcatggctaa
     3121 aacgttatac gaaaaattat tcgacgctca cgtagtgtac gaagccgaaa acgaaacccc
     3181 gctgttatat atcgaccgcc acctggtgca tgaagtgacc tcaccgcagg cgttcgatgg
     3241 tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa accttcgcca ctatggatca
     3301 caacgtctct acccagacga aagacattaa tgcctgtggt gaaatggcgc gcatccagat
     3361 gcaggagctg attaaaaact gcaaagaatt tggcgtcgaa ctgtatgacc tgaatcaccc
     3421 gtatcagggg atcgtccacg taatggggcc ggaacagggc gtcaccttgc cggggatgac
     3481 cattgtctgc ggcgactcgc ataccgccac ccacggcgcg tttggcgcac tggcctttgg
     3541 tatcggcact tccgaagttg aacacgtact ggcaacgcaa accctgaaac agggccgcgc
     3601 aaaaaccatg aaaattgaag tccagggcaa agccgcgccg ggcattaccg caaaagatat
     3661 cgtgctggca attatcggta aaaccggtag cgcaggcggc accgggcatg tggtggagtt
     3721 ttgcggcgaa gcaatccgtg atttaagcat ggaaggtcgt atgaccctgt gcaatatggc
     3781 aatcgaaatg ggcgcaaaag ccggtctggt tgcaccggac gaaaccacct ttaactatgt
     3841 caaaggccgt ttgcatgcgc cgaaaggcaa agatttcgac gacgccgttg cctactggaa
     3901 aaccctgcaa accgacgaag gcgcaacttt cgataccgtt gtcactctgc aagcagaaga
     3961 aatttcaccg caggtcacct ggggcaccaa tccaggccag gtgatttccg tgaacgacaa
     4021 tattcccgat ccggcttcgt ttgccgatcc ggttgaacgc gcgtcggcag aaaaagcgct
     4081 ggcctatatg gggctgaaac cgggcattcc gctgaccgaa gtggctatcg acaaagtgtt
     4141 tatcggttcc tgtaccaact cgcgcattga agatttacgc gcggcagcgg agatcgccaa
     4201 agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt cccggctctg gccaggtaaa
     4261 agcccaggcg gaagcggaag gtctggataa aatctttatt gaagccggtt ttgaatggcg
     4321 cttgcctggc tgctcaatgt gtctggcgat gaacaacgac cgtctgaatc cgggcgaacg
     4381 ttgtgcatcc accagcaacc gtaactttga aggccgccag gggcgcggcg ggcgcacgca
     4441 tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc ggacatttcg ccgacattcg
     4501 caacattaaa taaggagcag accatggcag agaaatttat caaacacaca ggcctggtgg
     4561 ttccgctgga tgccgccaat gtcgataccg atgcaatcat cccgaaacag tttttgcaga
     4621 aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga ctggcgtttt ctcgatgaaa
     4681 aaggccaaca gccaaacccg gacttcgtgc tgaacttccc gcagtatcag ggcgcttcca
     4741 ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg tgagcacgcg ccctgggcat
     4801 tgaccgacta cggttttaaa gtggtgattg cgccgagttt tgctgacatc ttctacggca
     4861 atagctttaa caaccagctg ctgccggtga aattaagcga tgcagaagtg gacgaactgt
     4921 ttgcgctggt gaaagccaat ccggggatcc atttcgacgt ggatctggaa gcgcaagagg
     4981 tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc cttccgccgc cactgcatga
     5041 tgaacggtct ggacagtatt gggcttacct tgcagcacga cgacgccatt gccgcttatg
     5101 aagcaaaaca acctgcgttt atgaattaat ccccttgccc ggtcaaatga ccgggctttc
     5161 cgctatcgtc cacgtcatca aacgtcttta acctttgcgg ttaaaaataa tgcgacaaca
     5221 gaaataaccg caattaccca gtagacagca aagtgccccc aactttgtgc aattgctccc
     5281 tgaataacgc cagccagaat taccccggta gaaatactgt tagtaaataa ggtggtagct
     5341 gcccccgctc ttccaggcat taaatcctga aaccatagca tcccaatacc cgcaacaatg
     5401 ccgataaata cagcgttaaa aagttgcagc gtcatcaacg ccatacggct atgaaagaaa
     5461 atcaatccgg tgtaaaacag tactcctgcc gccactgcta tgaccatcat tcgccgctta
     5521 ccataacgtt tgacatagta gccagccaga atcattgctg gtatttccag tccagctgcc
     5581 gtccccatca ggaaacccgc cagtttgtct ggcaatccta actcgctact gatccacaac
     5641 ggcatatcaa taatgtacat ggtgttgcag gtccacatta acgtcgaggc gacaaataac
     5701 atccgtacgt tactatcctg ccagccacct tgcattgata aagcattttc cgacggcagt
     5761 tctacccgcg ccacagacgg aagcataaat gcaatcaata ccagactgag tgtgaatatc
     5821 ccggcggcaa tcgaaaacat caccacttct cgcgccgagt tatcctcata ttcccgcgcc
     5881 agagcaaata actgtggcat tgccgtattg gccagagatg ccagaagcac accacaggtg
     5941 ataagcgtca gataatgacg attaaatgca aacaatggcg cattgccgat agccatcaaa
     6001 cagcaaaata taatcagttt tcgccgatcg ccctgactgt cagaacgttt tgccaaccag
     6061 aggcttacgc cgatcccagc aatagtattc accgtataaa agagtccgat ccagaaaggt
     6121 tgcgcgccaa cctcacgact cagaaataag ctcaatgtag gagcctgtag cgccccggcc
     6181 acccccatca taaaagcgac cagcataaat gccgcgtaaa caccgttcat acggcgagcc
     6241 atcgtcatta tccagatcat acgttccctt tttagcgcgg cgagaataaa aaaaccagca
     6301 ggtataatct gctggcgggt gattttacac caatactcag tcacacatga tgcaggcaag
     6361 tcaactttca gaattgcggt catcccactg catcagtcct tcaagcatgg ttaatcgttg
     6421 tgggacgctt aaccaacgca accagcacaa cttcgctgtc gcggtaaaat agtgctgata
     6481 aaactgacgc atggggcacc tccttgcttc atcgttagga attattgact taatataggg
     6541 aaaataaaat tgctgtcttt tgcgcaggag ttcccctttt atgccatctg ctcgtctgca
     6601 acaacagttc atccgcctgt ggcaatgctg cgagggtaaa tcgcaggaca caacgctcaa
     6661 cgaactggca gcgttattga gctgctcgcg tcgtcatatg cgtaccctgc tcaacaccat
     6721 gcaggatcgc ggctggctga cgtgggaagc ggaagtcggg cgcggtaaac gctcgcgtct
     6781 gacattcctc tataccgggc tggcgcttca gcaacagcgg gcggaagacc tgctggagca
     6841 ggatcgtatc gaccaactag tgcagttggt tggcgacaaa gcgactgtgc ggcaaatgct
     6901 ggtttctcat ctgggccgca gcttccgcca ggggcggcac atcctgcgcg tgctctacta
     6961 tcgtccgttg cgtaatctgc tacctggcag cacattgcgc cgttccgaaa cccatattgc
     7021 ccggcaaatc ttcagttcgc taacgcgcat aaatgaggaa aatggggaac tggaagcaga
     7081 catcgcccac cactggcagc aaatatcacc gcttcactgg cgtttctttt tgcgtccagg
     7141 agtccatttt catcatggtc gtgaactgga aatggacgac gtgatcgcct ctttaaaacg
     7201 aatcaatacg ctgccgctct attcgcatat tgcggacatt gtgtcgccga cgccctggac
     7261 gctggatatc catctcacgc aaccggaccg ctggttaccg ttactgctgg ggcaagttcc
     7321 ggcgatgatc ctgccgcgcg aatgggaaac cctcagtaac tttgccagcc atcccatcgg
     7381 caccggtccg tatgcggtga tccgcaacac caccaatcaa ctgaaaattc aggcattcga
     7441 tgacttcttc ggttaccggg cattaatcga tgaagttaac gtctgggttc tgccggaaat
     7501 tgccgacgag ccagctggag gtctgatgct aaaaggtcca cagggtgagg aaaaagagat
     7561 tgaaagccgc ctggaggaag gttgctacta tttactattc gacagccgca cccatcgcgg
     7621 agcgaatcag caagtcaggg actgggtaaa ctatgtgctt tctccaacta atctggtcta
     7681 tttcgctgag gaacagtacc agcaactgtg gttcccggct tatggactgc tcccccgttg
     7741 gcaccatgcc cgcaccataa agagcgaaaa accggctggc ctggaaagcc tcaccctgac
     7801 cttttatcag gatcacagtg agcatcgggt gattgccggg atcatgcagc agattctggc
     7861 aagtcaccag gtcacgctgg aaatcaaaga gatcagctac gatcagtggc atgaaggaga
     7921 gatcgagagc gatatctggc ttaatagcgc caactttacg ctgccgctgg atttttcgct
     7981 gttcgcgcac ttgtgcgagg tgccgctgct ccaacactgt attccgatcg actggcaagc
     8041 cgacgccgcc cgctggcgca atggcgaaat gaacctggcg aactggtgcc agcaactggt
     8101 cgccagtaaa gcaatggtgc cgcttatcca ccactggctg atcattcagg gacaacgcag
     8161 tatgcgcggc ctgcgtatga ataccctcgg ctggttcgat tttaaatcag cgtggtttgc
     8221 gccgccggat ccatgattgc tggtagataa caaaatcact acactaacgc cgttctcaac
     8281 ggggtgccac gcaaacgcgt gcgctgagaa aatacccgtc gaacctgatc cggataacgc
     8341 cggcgaaggg atttgaggct ccttctcaag tcctttgcca ctcttttttg aggtgcaaag
     8401 tgttaaaaaa atgtcttccc ctgctgttgc tgtgcacagc gcccgttttc gctaaacccg
     8461 ttctgactgt ttatacctac gattccttcg ccgccgactg ggggcctggt ccaaagatca
     8521 aaaaagcttt tgaagccgac tgtaactgcg aactgaaact ggtggcgctg gaagatggcg
     8581 tttcgcttct caaccgtcta cggatggaag gcaaaaatag taaagccgat gtggtgctgg
     8641 ggctggataa caatctgtta gacgccgcca gtaaaaccgg actatttgcc aaaagcggtg
     8701 tggcagcgga tgccgttaac gttcccggcg gctggaataa tgacactttc gtcccgtttg
     8761 attacggcta tttcgccttc gtctatgaca agaacaaact gaaaaacccg ccgcaaagcc
     8821 tgaaagaatt ggttgagagc gatcaaaact ggcgggtgat ttatcaggat ccgcgcacca
     8881 gtacgccggg gctgggcctg ctgttgtgga tgcaaaaagt ctatggcgat gacgccccac
     8941 aagcctggca aaaactggcg aagaaaaccg tcacggtcac caaaggctgg agcgaagcct
     9001 acggcctgtt tttaaaaggt gaaagcgatc tggtactgag ttacaccacc tctccggctt
     9061 atcacattct cgaagagaaa aaagataact acgccgccgc gaactttagc gaaggtcact
     9121 atctgcaggt ggaagtcgcc gcccgcaccg ctgccagcaa gcagccggag ctggcgcaaa
     9181 aattcctcca gtttatggtt tctccggctt ttcagaatgc gatcccaacc ggtaactgga
     9241 tgtatccggt ggcaaatgtc acgctgcctg ccgggtttga acaattgacc aaaccggcca
     9301 ccacgctgga gttcacacca gctgaagtgg cggcacaacg tcaggcatgg attagcggat
     9361 ggcaacgcgc cgtcagccgt taattcccgg ctggttaatt ccaggtgtaa gcgccgccac
     9421 gctggtggta gcggttgcgc tcgcggcgtt tctcgccctg tggtggaacg cgccgcaggg
     9481 taactgggtg gcagtctggc aggacagcta cctgtggcat gtggtgcgct tctccttctg
     9541 gcaggcgttt ctctcggcgc tactctctgt cgtacccgcg atattcctcg cccgcgccct
     9601 ctatcgcagg cgctttccgg gtcggctggc gctgttgcgt ctgtgcgcaa tgaccttgat
     9661 cctcccggtg ctggttgctg ttttcggcat tcttagcgtc tatggtcgcc agggctggct
     9721 ggcatcgctc tgccaatcgc tcggtctgga gtggaccttt tcgccctacg gcctgcaagg
     9781 tattttgctg gcgcacgtat tttttaatct gccgatggcg agccgcttat tactccaggc
     9841 actggaaaac attcccgacg aacaacgtca gcttgccgcc cagcttggga tgcgcggctg
     9901 gcattttttc cgcttcgtcg aatggccgtg gttacggcga caaatcccgc cggttgctgc
     9961 acttatcttt atgctctgtt tcgccagctt cgccaccgtg ctatcactgg gcggcggtcc
    10021 gcaggcgacc actatcgagc tggctattta tcaggcgctg agttacgact acgatcctgc
    10081 ccgcgcggcg atgctggcgc tgatccagat ggtgtgttgc cttgggctgg tactgttgag
    10141 tcagcgattg agtaaggcca ttgcgccagg caccacgctg ctgcaaggct ggcgcgaccc
    10201 ggacgatcgt ctgcatagcc gcatttgcga cacggtgtta atagtgctgg cgttgctgct
    10261 gttgttgcca ccgttgctgg cggtgatcgt cgatggggta aatcgccagt tgccggaagt
    10321 gctggcacaa ccggtgctgt ggcaggcgct gtggacctcg ttgcgtattg cgctggcggc
    10381 aggtgtattg tgcgtagtgc tgaccatgat gctgctatgg agcagtcgcg aacttcgggc
    10441 acggcagaaa atgctggcgg gtcaggcgct ggagatgagc ggcatgttga ttctcgccat
    10501 gccggggatt gtgctggcta ccggcttctt tttactgctc aacaacacta tcggcctgca
    10561 acaatctgct gacggcattg tgattttcac caatgcgtta atggcgatcc cttatgcgct
    10621 gaaagtactg gaaaacccga tgcgcgatat caccgcccgc tacagtatgt tgtgtcagtc
    10681 gctgggcatt gaaggctggt cgcgcttaaa agtggtggag ctgcgcgccc tgaaacgtcc
    10741 actggcgcag gcgctggcct ttgcctgcgt gctgtcgatt ggtgattttg gcgtggtggc
    10801 gttgttcggt aacgatgatt tccgtaccct gccgttttat ctctaccagc aaattggctc
    10861 ctatcgcagc caggacggcg cggtcaccgc gttaattctg ctgctactct gttttctgct
    10921 gtttaccgtg attgaaaaac taccggggcg aaatgttaaa actgactgat atcacctggc
    10981 tttaccaaca tttgccgatg cgttttagct taacggtgga acgcggcgag caggtggcga
    11041 tcctcgggcc aagcggcgca ggtaaaagta ccctgctgaa tttgattgcc ggttttctga
    11101 cgccagccag cggttcgctg actatcgatg gcgttgatca caccacgacg ccgccgtcac
    11161 gccgtccggt gtcgatgctg tttcaggaga acaacctgtt cagccacctg acggtcgcgc
    11221 agaacatcgg gctggggctt aatccaggat tgaagctgaa cgcggcacag caggagaaaa
    11281 tgcacgctat cgcccgtcag atggggattg ataatttaat ggcgcggtta ccgggcgagc
    11341 tttccggcgg ccagcgacag cgcgtggcgt tagcgcgttg tctggtgcgc gaacagccga
    11401 ttttattgct cgatgaaccg ttctctgcgc tcgatccggc gttacgtcag gagatgctga
    11461 cgctggtgag cacgagctgc cagcagcaaa aaatgacgct attgatggtg tcgcacagtg
    11521 tggaagatgc ggcgcggatc gccacacgct cggtggtggt cgccgacggg cgcatcgcct
    11581 ggcagggtaa gactgaagaa ttgttgagcg gtaaggcgag tgcttcggca atattgggga
    11641 ttacgggtta gtatgcactg tcagatgcgg cgtgaacacc ttatccgacc tacggacagt
    11701 gcgttttgta ggcctgataa gaccggtcag cgtcgcatca ggcaacccgc acaagacaat
    11761 cccctaaacc ccaaccactt tacgcaaaat atcgatatac accggcatca acgggtggcg
    11821 aattaacacc accagcgcca ccacgccgat ggcagaaatc aacggcgtca gccacaacaa
    11881 acggccgcgc gacaaataat gacttaaacg atcggttgct ttaccgctgc gccataaccg
    11941 ccagcacagc cagccaccaa cccacaaaaa taccgccgtc gccagcagca accatttaaa
    12001 ctcgccgctc tgcattccgg ctggaatatc gatcgccgcg cccgccagaa tcccaggcag
    12061 gaagtaaaac ggtggccaca gcaggcagcc gataatattc ggcgtaatga attttgccac
    12121 cggcagatcc agcattcccg ccaccattgg caccagcgga cgcgtcgggc caacaaagcg
    12181 accgaccaga atggtgaaca tgctgtgttg atgcaacgca tgttcagttt tatcgagtaa
    12241 tgctttgttt ttcttcagaa atgaccagcg atgcaacggc tttttaaaac gccagcccag
    12301 ccagaaggag atccagtcgc ccagcagaca accgacaatc cctgccagcc aggcgtgcca
    12361 gaaacttaac tcgccgctgc caatcagcgc tcccagcccc gccatcagca ccgtaccggg
    12421 tagaatcaaa ccgaccagcg ccagcgactc cagaaaggcc accaacacca ccgccatcaa
    12481 tgaatacacg gtagattggg taataaagtg ttccagcaat gcttgcataa tgtgcctgtc
    12541 aaatggacga agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct
    12601 gattcgttac caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg
    12661 cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt
    12721 gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa
    12781 gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact
    12841 gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg
    12901 cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc
    12961 gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt
    13021 gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa
    13081 tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaaac
    13141 ccgtattggc aaatattgac ggccagttaa gccattcatg ccaataggcg cgcggacgaa
    13201 agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct
    13261 ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt
    13321 tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc
    13381 ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat
    13441 gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca
    13501 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
    13561 gcgtctttta ctggctcttc tcgctaaccc aaccggtaac cccgcttatt aaaagcattc
    13621 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
    13681 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctattcc atagcatttt
    13741 tatccataag attagcggat cctacctgac gctttttatc gcaactctct actgtttctc
    13801 catacccgtt tttttggatg gagtgaaacg atggcgattg caattggcct cgattttggc
    13861 agtgattctg tgcgagcttt ggcggtggac tgcgccagcg gtgaagagat cgccaccagc
    13921 gtagagtggt atccccgttg gcaaaaaggg caattttgtg atgccccgaa taaccagttc
    13981 cgtcatcatc cgcgtgacta cattgagtca atggaagcgg cactgaaaac cgtgcttgca
    14041 gagcttagcg tcgaacagcg cgcagctgtg gtcgggattg gcgttgacag taccggctcg
    14101 acgcccgcac cgattgatgc cgacggtaac gtgctggcgc tgcgcccgga gtttgccgaa
    14161 aacccaaacg cgatgttcgt attgtggaaa gaccacactg cggttgaaga agcggaagag
    14221 attacccgtt tgtgccacgc gccgggcaac gttgactact cccgctacat tggtggtatt
    14281 tattccagcg aatggttctg ggcaaaaatc ctgcatgtga ctcgccagga cagcgccgtg
    14341 gcgcaatctg ccgcatcgtg gattgagctg tgcgactggg tgccagctct gctttccggt
    14401 accacccgcc cgcaggatat tcgtcgcgga cgttgcagcg ccgggcataa atctctgtgg
    14461 cacgaaagct ggggcggctt gccgccagcc agtttctttg atgagctgga cccgatcctc
    14521 aatcgccatt tgccttcccc gctgttcact gacacctgga ctgccgatat tccggtgggc
    14581 accttatgcc cggaatgggc gcagcgtctc ggcctgcctg aaagcgtggt gatttccggc
    14641 ggcgcgtttg actgccatat gggcgcagtt ggcgcaggcg cacagcctaa cgcactggta
    14701 aaagttatcg gtacttccac ctgcgacatt ctgattgccg acaaacagag cgttggcgag
    14761 cgggcagtta aaggtatttg cggtcaggtt gatggcagcg tggtacctgg atttatcggt
    14821 ctggaagcag gccaatcggc gtttggtgat atctacgcct ggtttggtcg cgtactcggc
    14881 tggccgctgg aacagcttgc cgcccagcat ccggaactga aagcgcaaat caacgccagc
    14941 cagaaacaac tgcttccggc gctgaccgaa gcatgggcca aaaatccgtc tctggatcac
    15001 ctgccggtgg tgctcgactg gtttaacggc cgccgcacac cgaacgctaa ccaacgcctg
    15061 aaaggggtga ttaccgatct taacctcgct accgacgctc cgctgctgtt tggcggtttg
    15121 attgctgcca ccgcctttgg cgcacgcgca atcatggagt gctttaccga tcaggggatc
    15181 gccgtcaata acgtgatggc gctgggcggc atcgcgcgga aaaaccaggt cattatgcag
    15241 gcctgctgcg acgtgctgaa tcgcccgctg caaattgttg cctctgacca gtgctgtgcg
    15301 ctcggtgcgg cgatttttgc tgccgtcgcc gcgaaagtgc acgcagacat cccatcagcc
    15361 cagcaaaaaa tggccagtgc ggtagagaaa accctgcaac cgcgcagcga acagacacaa
    15421 cgctttgaac agctttatcg ccgctatcag caatgggcga tgagcgccga acaacactat
    15481 cttccaactt ccgccccggc acaggctgcc caggccgttc cgactctata aggacacgat
    15541 aatgacgatt tttgataatt atgaagtgtg gtttgtcatt ggcagccagc atctgtatgg
    15601 cccggaaacc ctgcgtcagg tcacccaaca tgccgagcac gttgttaatg cgctgaatac
    15661 ggaagcgaaa ctgccctgca aactggtgtt gaaaccgctg ggcaccacgc cggatgaaat
    15721 caccgctatt tgccgcgacg cgaattacga cgatcgttgc gctggtctgg tggtgtggct
    15781 gcacaccttc tccccggcca aaatgtggat caacggcctg accatgctca acaaaccgtt
    15841 gctgcaattc cacacccagt ttaacgcggc gctgccgtgg gacagtatcg atatggactt
    15901 tatgaacctg aaccagactg cacatggcgg tcgcgagttc ggcttcattg gcgcgcgtat
    15961 gcgtcagcaa catgccgtgg ttaccggtca ctggcaggat aaacaagccc atgagcgtat
    16021 cggctcctgg atgcgtcagg cggtctctaa acaggatacc cgtcatctga aagtctgccg
    16081 ttttggcgat aacatgcgtg aagtagcggt caccgatggt gataaagttg ccgcacagat
    16141 caagttcggt ttctccgtca atacctgggc ggttggcgat ctggtgcagg tggtgaactc
    16201 catcagcgac ggcgatgtta acgcgctggt cgatgagtac gaaagctgct acaccatgac
    16261 gcctgccaca caaatccacg gcgaaaaacg acagaacgtg ctggaagcgg cgcgtattga
    16321 gctggggatg aagcgtttcc tggaacaagg tggcttccac gcgttcacca ccacctttga
    16381 agatttgcac ggtctgaaac agcttccagg tctggccgta cagcatctga tgcagcaggg
    16441 ttacggcttt gcgggcgaag gcgactggaa aaccgccgcc ctgcttcgca tcatgaaggt
    16501 gatgtcaacc ggtctgcagg gcggcacctc ctttatggag gactacacct atcacttcga
    16561 gaaaggtaat gacctggtgc tcggctccca tatgctggaa gtctgcccgt cgattgccgt
    16621 agaagagaaa ccgatcctcg acgttcagca tctcggtatt ggtggtaagg acgatcctgc
    16681 ccgactgatc ttcaataccc aaaccggtcc agcgattgtc gccagcctga ttgatctcgg
    16741 cgatcgttac cgtctgctgg ttaactgtat cgacacggtg aaaacaccgc actccctgcc
    16801 gaaactgccg gtggcgaatg cgctgtggaa agcgcaaccg gatctgccaa ctgcttccga
    16861 agcgtggatc ctcgctggtg gcgcgcacca taccgtcttc agccatgcgc tgaacctcaa
    16921 cgatatgcgt cagttcgccg agatgcacga cattgaaatc acggtgattg ataacgacac
    16981 ccgcctgcca gcgtttaaag acgcactgcg ctggaacgaa gtgtattacg gatttcgtcg
    17041 ctaagtagcc acatcaggta tgtaacgcct gatgcgacgc tgacgcgtct tatctggcct
    17101 acacgctgcg attttgtagg ccggataagc aaagcgcatc cggcattcaa cgcctgatgc
    17161 gacgctggcg cgtcttatca ggcctacgcg ctgcgatttt gtagaccgga taagcgaagc
    17221 gcatccggca cgaaggagtc aacatgttag aagatctcaa acgccaggta ttagaagcca
    17281 acctggcgct gccaaaacac aacctggtca cgctcacatg gggcaacgtc agcgccgttg
    17341 atcgcgagcg cggcgtcttt gtgatcaaac cttccggcgt cgattacagc gtcatgaccg
    17401 ctgacgatat ggtcgtggtt agcatcgaaa ccggtgaagt ggttgaaggt acgaaaaagc
    17461 cctcctccga cacgccaact caccggctgc tctatcaggc attcccctcc attggcggca
    17521 ttgtgcatac gcactcgcgc cacgccacca tctgggcgca gtcgggtcag tcgattccag
    17581 caaccggcac cacccacgcc gactatttct acggcaccat tccctgcacc cgcaaaatga
    17641 ccgacgcaga aatcaacggc gaatatgagt gggaaaccgg taacgtcatc gtagaaacct
    17701 ttgaaaaaca gggtatcgat gcagcgcaaa tgcccggcgt tctggtccat tcccacggcc
    17761 cgtttgcatg gggcaaaaat gccgaagatg cggtgcataa cgccatcgtg ctggaagagg
    17821 tcgcttatat ggagatattc tgccgtcagt tagcgccgca gttaccggat atgcagcaaa
    17881 cgctgctgga taaacactat ctgcgtaagc atggcgcgaa ggcatattac gggcagtaat
    17941 gactgtataa aaccacagcc aatcaaacga aaccaggcta tactcaagcc tggttttttg
    18001 atggattttc agcgtggcgc aggcaggttt tatcttaacc cgacactggc gggacacccc
    18061 gcaagggaca gaagtctcct tctggctggc gacggacaac gggccgttgc aggttacgct
    18121 tgcaccgcaa gagtccgtgg cgtttattcc cgccgatcag gttccccgcg ctcagcatat
    18181 tttgcagggt gaacaaggct ttcgcctgac accgctggcg ttaaaggatt ttcaccgcca
    18241 gccggtgtat ggcctttact gtcgcgccca tcgccaattg atgaattacg aaaagcgcct
    18301 gcgtgaaggt ggcgttaccg tctacgaggc cgatgtgcgc ccgccagaac gctatctgat
    18361 ggagcggttt atcacctcac cggtgtgggt cgagggtgat atgcacaatg gcactatcgt
    18421 taatgcccgt ctgaaaccgc atcccgacta tcgtccgccg ctcaagtggg tttctataga
    18481 tattgaaacc acccgccacg gtgagctgta ctgcatcggc ctggaaggct gcgggcagcg
    18541 catcgtttat atgctggggt cggagaatgg cgacgcctcc tcgcttgatt tcgaactgga
    18601 atacgtcgcc agccgcccgc agttgctgga aaaactcaac gcctggtttg ccaactacga
    18661 tcctgatgtg atcatcggtt ggaacgtggt gcagttcgat ctgcgaatgc tgcaaaaaca
    18721 tgccgagcgt taccgtcttc cgctgcgtct tgggcgcgat aatagcgagc tggagtggcg
    18781 cgagcacggc tttaaaaacg gcgtcttttt tgcccaggct aaaggtcggc taattatcga
    18841 cggtatcgag gcgctgaaat ccgcgttctg gaatttctct tcattctcgc tggaaactgt
    18901 cgctcaggag ctattaggcg aaggaaaatc tatcgataac ccgtgggatc gaatggacga
    18961 aattgaccgc cgtttcgccg aagataaacc tgcgctggca acttataacc tgaaagattg
    19021 cgagctggtg acgcagatct tccacaaaac tgaaatcatg ccatttttac tcgaacgggc
    19081 aacggtgaac ggcctgccgg tggaccgaca cggcggttcg gtggcggcat ttggtcatct
    19141 ctattttccg cgaatgcatc gcgctggtta tgtcgcgcct aatctcggcg aagtgccgcc
    19201 gcacgccagc cctggcggct acgtgatgga ttcacggcca gggctttatg attcagtgct
    19261 ggtgctggac tataaaagcc tgtacccgtc gatcatccgc acctttctga ttgatcccgt
    19321 cgggctggtg gaaggcatgg cgcagcctga tccagagcac agtaccgaag gttttctcga
    19381 tgcctggttc tcgcgagaaa aacattgcct gccggagatt gtgactaaca tctggcacgg
    19441 gcgcgatgaa gccaaacgcc agggtaacaa accgctgtcg caggcgctga aaatcatcat
    19501 gaatgccttt tatggcgtgc tcggcaccac cgcctgccgc ttcttcgatc cgcggctggc
    19561 atcgtcgatc accatgcgtg gtcatcagat catgcggcaa accaaagcgt tgattgaagc
    19621 acagggctac gacgttatct acggcgatac cgactcaacg tttgtctggc tgaaaggcgc
    19681 acattcggaa gaagaagcgg cgaaaatcgg tcgtgcactg gtgcagcacg ttaacgcctg
    19741 gtgggcggaa acgctgcaaa aacaacggct gaccagcgca ttagaactgg agtatgaaac
    19801 ccatttctgc cgttttctga tgccaaccat tcgcggagcc gataccggca gtaaaaagcg
    19861 ctacgccggg ctgattcagg agggcgataa acagcggatg gtgtttaaag ggctggaaac
    19921 cgtgcgcacc gactggacgc cgctggccca gcagtttcag caggagctat acctgcgcat
    19981 cttccgcaac gagccatatc aggaatatgt acgcgaaacc atcgacaaac tgatggcggg
    20041 tgaactggat gcgcgactgg tttaccgtaa acgccttcgc cgtccgctga gcgagtatca
    20101 gcgtaatgtg ccgcctcatg tacgcgccgc tcgccttgcc gatgaagaaa accaaaagcg
    20161 tggtcgcccc ttgcaatatc agaatcgcgg caccattaag tacgtatgga ccaccaacgg
    20221 cccggagccg ctggactacc aacgttcacc actggattac gaacactatc tgacccgcca
    20281 gctacaaccc gtggcggagg gaatactccc ttttattgag gataattttg ctacacttat
    20341 gaccgggcaa cttgggctat tttgagcaaa aaaagagttc gccagatacc attttgatgc
    20401 gtgacgaatg ctttgccatc cagtaccata gcgccctttc cattcctgga cctgaataac
    20461 accactacct cataagcacg gtagcgggtg gttattgcct gcaattaaag atatagagcc
    20521 gaacacatat gccttttaca cttggtcaac gctggatcag cgatacagaa agcgaattgg
    20581 gacttggaac cgttgtcgcg gtggatgcgc gaactgtcac tttacttttc ccatctactg
    20641 gtgaaaaccg tctgtacgca cgcagtgatt cccccgtgac ccgcgtgatg ttcaaccctg
    20701 gtgataccat taccagccat gacggctggc agatgcaagt cgaagaagta aaagaagaaa
    20761 atggcttgct gacctatatc ggtactcgcc tggatactga agagtccggc gtagccctgc
    20821 gtgaagtttt ccttgatagc aaactggtgt tcagcaaacc gcaggaccgc ctgtttgccg
    20881 ggcagattga ccgtatggac cgctttgcgc tgcgttatcg cgcgcgtaag tattccagcg
    20941 aacagttccg tatgccgtac agcggcctgc gcggtcagcg taccagcctg atcccgcacc
    21001 agctcaacat cgctcatgat gttggccgcc gccacgcgcc gcgcgtcctg ctggctgacg
    21061 aagtgggttt agggaaaacc attgaagccg ggatgatcct gcatcagcaa ctgctctctg
    21121 gcgctgctga acgtgtgctg attatcgtcc cggaaacctt acagcatcag tggctggtag
    21181 aaatgctgcg ccgtttcaac ctgcgctttg ctctgtttga tgatgagcgt tatgccgaag
    21241 ctcagcacga tgcttacaac ccgttcgaca ccgaacagct ggtgatttgc tcgctggatt
    21301 ttgcccgtcg tagcaaacag cgcctggaac atctctgtga agccgaatgg gacctgctgg
    21361 tggtcgatga agcgcatcac ctggtgtgga gcgaagatgc gccaagccgt gaatatcagg
    21421 ccattgaaca actggcagag cacatgccgg gcgttctgct gctgaccgcg accccggaac
    21481 agctggggat ggaaagccac ttcgcccgtc tgcgtctgct ggacccgaac cgtttccacg
    21541 attttgcgca gttcgttgaa gagcagaaaa attatcgtcc ggttgcggac gccgttgcca
    21601 tgctgctggc aggtaacaaa ctgagcaatg acgaactgaa catgctcggc gagatgatcg
    21661 gcgagcagga tatcgagccg ctgttgcagg cagcaaacag cgacagcgaa gatgcccaga
    21721 gcgcccgtca ggagctggtt tcgatgctga tggatcgcca cggcaccagc cgcgtgctgt
    21781 tccgtaacac gcgtaacggt gtgaaaggat tcccgaaacg cgagctgcac accattaagc
    21841 tgccgctacc gacgcagtat cagacggcta ttaaagtctc cggcattatg ggcgcacgta
    21901 aaagtgcgga agatcgtgct cgcgatatgc tctacccgga gcgtatttat caggaatttg
    21961 aaggtgataa cgccacctgg tggaacttcg atccgcgcgt tgagtggctg atgggctacc
    22021 tgaccagcca tcgctctcag aaagtgctgg tgatctgcgc caaagctgcc actgcgctgc
    22081 aactggagca ggtactgcgc gaacgtgaag gtattcgcgc tgcggtgttc cacgaaggta
    22141 tgtcgattat cgaacgtgac cgcgctgccg cctggtttgc cgaagaagac accggcgcac
    22201 aggtactgct gtgctcagaa atcggttctg aaggacgtaa cttccagttc gccagccaca
    22261 tggtgatgtt tgacctgcca ttcaacccgg atctactgga gcagcgtatt ggtcgtctgg
    22321 atcgtatcgg ccaggcgcac gatattcaga tccatgtgcc ttatctggag aaaaccgctc
    22381 agtcggtgct ggtgcgctgg tatcacgaag gtctggatgc atttgagcac acctgcccga
    22441 ccggacgcac tatttacgat agcgtataca acgatctgat taactatctg gcttcaccgg
    22501 atcaaaccga aggctttgac gatctgatca aaaactgccg cgagcaacat gaagcgctga
    22561 aagcacagct ggaacagggt cgtgaccgcc tgctggaaat ccactccaac ggtggcgaaa
    22621 aagcccaggc actggcagaa agcattgaag agcaggatga cgataccaac ctgatcgcct
    22681 tcgccatgaa cctgctcgat attatcggta tcaatcagga cgatcgcggc gacaacatga
    22741 tcgtgctgac gccgtccgat catatgctgg tgccggactt ccctggcctg tcggaagatg
    22801 gcatcaccat cacctttgat cgtgaagtgg cgctggcgcg tgaagatgca cagtttatta
    22861 cctgggagca tccgctgatc cgcaacggtc tggatctgat cctttctggc gataccggta
    22921 gcagcacgat ttcactgtta aaaaacaaag cgttgccggt aggtacgctg ttggtggaac
    22981 tgatttatgt ggttgaagcc caggctccga agcagttgca gctcaaccgc ttcctgccac
    23041 cgacgccggt acgtatgctg ctggataaaa acggcaacaa cctggcggcg caggtagagt
    23101 ttgaaacctt taaccgccag cttaacgcgg ttaaccgtca caccggcagc aaactggtta
    23161 acgccgtgca gcaggatgtt cacgctatcc ttcaactggg tgaagcgcag atcgagaaat
    23221 ctgcccgtgc attgattgat gcagcgcgta acgaagccga cgaaaaactg tctgccgagc
    23281 tgtctcgtct ggaagctctg cgtgcagtga acccgaacat tcgtgacgac gaactgaccg
    23341 ccattgagag caaccgtcag caggtaatgg aaagcctgga tcaggcaggt tggcgtctgg
    23401 atgccctgcg tttgatcgtt gtaacgcatc agtaacggag ccgaaaatgg ggatggaaaa
    23461 ctacaatcca ccgcaggaac cctggttggt tatcctgtat caggatgacc acattatggt
    23521 ggttaacaag ccgagcggtt tgttgtctgt gccggggcgt ctggaagagc acaaagacag
    23581 cgtgatgacg cgcattcagc gtgattatcc gcaggcagaa tcggtgcatc gtctggatat
    23641 ggctaccagc ggcgtgattg tagtggcgct gaccaaagcc gcggagcggg agttaaaacg
    23701 ccagttccgc gagcgcgagc cgaaaaagca gtatgtggcc cgcgtctggg ggcatccatc
    23761 ccctgcggaa ggtctggtgg atctgccgct gatttgcgac tggccaaacc gcccgaaaca
    23821 gaaagtctgt tacgaaacgg gtaaacctgc gcagacggaa tatgaagtgg tggagtatgc
    23881 ggcggataac acggcaagag tggtgttaaa accgattact gggcgttcgc atcaattgcg
    23941 tgtgcatatg ctggcgctgg ggcatccgat tctcggcgat cgtttttatg catcaccaga
    24001 agcgagagcg atggcaccgc gtttgttgct acatgcagag atgttgacga ttacccatcc
    24061 ggcgtatggc aatagtatga cgtttaaagc gccagcggat ttttaatagt cacgctggcg
    24121 cagttcggta ggcggataag gcgttcacgc cgaatccggc aattgtgcgc cgattgcctg
    24181 atgcgacgcg ggccgcgtct tatcaggcct acagtttaaa acttatttaa accctttctg
    24241 ctgctttatc agctcatatg cctgctgaat ttcctgcgct ttctgcttcg ccatctccat
    24301 catctcaggc ggcaaacctt tcgccaccag cttatcggga tggtgttcac tcatcagctt
    24361 acggtaggct cgtttaatgg tggtcgcatc atccgtcggc ttcacaccca gcacattaca
    24421 ggcatcttcc agcgtcgggc cacgctgcgc ttgctgccag ttaccaccgc cagattgctg
    24481 ctgataaccg ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg
    24541 ggcgcgggag atccctaatt cttctgcaat gacatacagc accgcccgtt cattcgggtg
    24601 cagtgaacca tcagcaaacg ctgcctgaat ctggatctcc agaaacatac gaattaagtc
    24661 aaaacgacca aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattgtc
    24721 tgattttccc acccggaacg cgttttgcgc cgcagtacgg gaagcgccat gaaggttcat
    24781 tcggtccatc agctggctgg cgatatgaat atcagcctcc gtgacgcgac ctttggattt
    24841 ggttaaatgc cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg
    24901 gttggcgaac cacgccattt tacggctacg ggctttatca aacatatggc caattaacag
    24961 gcctaacact acgccccaaa agccgccgcc catcagtaag gccacggcca cgccaatgat
    25021 ttttccccaa tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca
    25081 caatataaag gtgcttttac cgttttccgg ctgcagtcaa ttgtgggaca tcgcctataa
    25141 tttgcattat catacctgtc attcaatgcc gtgcctaaca ccactgacgc tattcggaca
    25201 ggattaacac tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa
    25261 cgttaccgat gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg
    25321 ccaccatgat tgccaccgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac
    25381 agtgcatgtt gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact
    25441 tacccgtgac tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta
    25501 ctggcagcgt ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc
    25561 atcaaaaaga ggcaccagga caaccggagc cggtacgaac cgttgatgcg ctcggtaatg
    25621 tccattacga cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca
    25681 ccaaagatac caacgtctgg gaaggtgatt accagatggt gggtcgccag ggccgcggta
    25741 aagcggacct gatgaaacaa cgtggtgaaa accgctatac cattctggat aacggtagct
    25801 ttacctcctg tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg
    25861 accgcgaaga acaagttgcg gagatctgga acgcccgctt taaggtgggt ccagtaccga
    25921 tcttttatag cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga
    25981 tcccgaacgc caagtacacc accaccaact actttgagtt ctacctgcca tattactgga
    26041 acatcgcgcc aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaaca
    26101 tcatgtggga gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg
    26161 actatctgcc ttcagataaa gtctatgaag atgaacaccc gaacgatgac agttcacgtc
    26221 gttggttgtt ctactggcaa cactccgggg tcatggatca agtgtggcgt ttcaacgtag
    26281 actacaccaa ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca
    26341 gtactgacgg ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg
    26401 ccaccgtttc aaccaagcag ttccaggtct ttagcgagca gaacaccagt agctactcgg
    26461 cagagccgca gttagacgtt aactactacc agaatgatgt tggtccgttt gatacgcgta
    26521 tttacggcca ggcagtgcac tttgttaaca ccagagacga catgcctgaa gcaacccgtg
    26581 ttcacctgga accgaccatc aatttgccgc tctctaataa ctggggcagc atcaataccg
    26641 aagcgaagtt gctggcaacc cattatcagc aaaccaatct tgactggtat aactccagaa
    26701 acacgaccaa gctggacgaa tccgttaacc gcgttatgcc gcaattcaaa gttgacggca
    26761 aaatggtctt tgaacgcgat atggaaatgc tggctccggg ttatacccaa acgctggaac
    26821 cgcgcgcgca gtatttgtac gtgccgtatc gcgatcagag cgacatctat aactacgact
    26881 cgtctctact gcaatctgac tactctggcc tgttccggga ccggacttac ggcggtcttg
    26941 accgtattgc ctccgctaac caggtgacga ccggtgtcac atctcgcata tatgatgatg
    27001 ctgccgttga acattttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca
    27061 ctggcgatga caacataaca tgggagaatg acgacaaaac gggctcactg gtgtgggcag
    27121 gcgatactta ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt cagtacgata
    27181 cacgtctgga taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc
    27241 gtctggtaca gctgaattac cgttacgcca gcccggaata tattcaggct acgctgccta
    27301 agtactattc cactgctgag caatataaga atggtatttc gcaggtaggt gctgtcgcca
    27361 gctggccaat tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga
    27421 acaagcaagc cgactctatg ttaggtgtgc aatacagctc ctgctgctat gcaattcgcg
    27481 tcggttacga gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca
    27541 acgcaatcgg ctttaacatc gaacttcgcg gcctgagctc caactacggt ctgggtacgc
    27601 aagagatgct gcgttcgaac attctgccgt atcaaaacac tttgtgattt gttgatttac
    27661 cacgtaatcc gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg
    27721 cttctcggta tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa
    27781 gtcgcagccg tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag
    27841 tcggtaaaac tgaacgctgc gcaggcaagg cagcagcttc ctgatgacgc gacgctgcgc
    27901 caccaaatca tggaacgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg
    27961 ggagtgaaaa tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac
    28021 aacatgacgc tggatcagat gcgcagccgt ctggcttacg atggtctgaa ctacaacacc
    28081 tatcgtaacc agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt
    28141 cgtcgcatca ccatcctgcc gcaggaagtc gaatccctgg cgcagcaggt gggtaaccaa
    28201 aacgacgcca gcactgagct gaacctgagc cacatcctga tcccgctgcc ggaaaacccg
    28261 acttctgatc aggtgaacga agcggaaagc caggcgcgcg ccattgtcga tcaggcgcgt
    28321 aacggcgctg atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac
    28381 ggcggccaga tgggctgggg ccgtattcag gagttgccgg gtatcttcgc ccaggcatta
    28441 agcaccgcga agaaaggcga cattgttggc ccgattcgtt ccggcgttgg cttccatatt
    28501 ctgaaagtta acgacctgcg cggcgaaagc aaaaatatct cggtgaccga agttcatgct
    28561 cgccatattc tgctgaaacc gtcgccgatc atgactgacg aacaggcccg tgtgaaactg
    28621 gaacagattg ctgccgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag
    28681 ttctctcagg atccaggctc tgctaaccag ggcggtgatc tcggctgggc tacaccagat
    28741 attttcgatc cggccttccg tgacgccctg acccgcctga acaaaggtca aatgagtgca
    28801 ccggttcact cttcattcgg ctggcattta atcgaactgc tggatacccg taatgtcgat
    28861 aaaaccgacg ctgcgcagaa ggatcgtgca taccgcatgc tgatgaaccg taagttctcg
    28921 gaagaagcag caagctggat gcaggaacaa cgcgccagcg cctacgttaa aatcctgagc
    28981 aactaatggt taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc
    29041 cggacttagt tgtccagctt gcacagcgtg agtggccggt cgaactggtt gtttgtgccg
    29101 atgccactct ccttaccgac cgggcagcga tgctcggttt gccgctcacc ctccgccctt
    29161 attcccccaa ctcccctgca caaccgcaaa ctgcgggcac attaacgcta cttcctgtcg
    29221 cgctacgcga atctgtcact gcggggcagt tagcgattga aaatgggcat tacgtggtgg
    29281 agacgctggc gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg ctgatcacag
    29341 gccccgtgca taaaggcgtc attaacgacg caggtattcc gtttactggt cataccgagt
    29401 ttttcgaaga gcgctcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc
    29461 gcgtggcgct ggcaacgacg catttaccgc tgcgcgatat cgcagatgct atcacccctg
    29521 cacttttgca cgaagtgatt gctattttgc atcacgattt gcgtacaaaa tttggtattg
    29581 ccgaaccgcg cattctggtc tgcgggctga atccgcacgc gggcgaaggc ggtcatatgg
    29641 gtacggaaga gatagacacc attattccgg tgctcgacga gctgcgggcg caggggatga
    29701 tactcaacgg gccgctgcct gccgataccc tgtttcagcc gaaatatctc gataacgccg
    29761 acgccgtgct ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg
    29821 ggcgcggtgt gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca
    29881 ccgcgcttga actggcggga cgtggcgaag ccgatgtcgg cagttttatt acggcgctta
    29941 atctcgccat caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt
    30001 agcccgtaaa cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt
    30061 ttctgccatt aacccgcaaa agggccaggc aatggtcgaa atcggccccg gtctggcggc
    30121 attgaccgag ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac ttgaccgcga
    30181 tctggcggca cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca
    30241 ggatgcgatg acctttaact ttggcgaact ggccgagaaa atgggtcagc cgctgcgtgt
    30301 tttcggcaac ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac
    30361 tgatgccatt gccgacatgc actttatgtt gcaaaaagag gtggtgaatc gtctggttgc
    30421 aggaccgaac agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact attgcaacgt
    30481 gatcccggta ctggaagttc cgccgtcagc ctttacacca ccacccaaag tggattccgc
    30541 cgtcgtgcgc ctggttcctc atgcaacgat gcctcacccg gttaaagatg ttcgtgtgtt
    30601 gagccgcatc accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg
    30661 caacctgttt agcgtcgagg tgttaacggg aatggggatc gacccggcga tgcgagcgga
    30721 aaatatctct gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt
    30781 gcaggagagt taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac
    30841 attgaggcac aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata
    30901 cgcaatctgg ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc
    30961 aatggccgtg aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg
    31021 cctggcgaag agtaccagta caccagcggt gcaatcattg aaaccccgct gggcaccatg
    31081 cagggtcact acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta
    31141 ttccgactcg ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc
    31201 gacgttcatg gttgttacga tgaactgatc gcattgctgc ataaagtaga atttacccct
    31261 gggaaagata ccctctggct gacgggcgat ctggtcgcgc gcggcccggg ttcgctggat
    31321 gttctgcgct atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg caatcacgat
    31381 ctgcatctgc tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca
    31441 ccgctgctgg aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctttg
    31501 ctgcaaatcg acgaagagaa aaagttggtg atggcccacg cagggatcac gccgcagtgg
    31561 gatctacaga ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctatc gagtgactcc
    31621 tatcccttct ttctcgacgc tatgtacggc gatatgccaa ataactggtc accggaattg
    31681 cgggggctgg gaagactgcg ttttatcacc aacgccttta cccgtatgcg tttttgcttc
    31741 ccgaacggtc aactggatat gtacagcaaa gaatcgccgg aagaggcccc tgccccactg
    31801 aaaccgtggt ttgcgattcc tggccctgtc gctgaagaat acagcatcgc ctttggtcac
    31861 tgggcatcgc tggagggcaa aggtacgccg gaaggtatat acgcgctgga taccggctgc
    31921 tgctggggtg gtacattaac ctgcctgcgc tgggaagata aacagtattt tgtccagccg
    31981 tcgaaccggc ataaggattt gggcgaagcg gcggcgtctt aaacacagcc tgatatagga
    32041 aggccggata agacgcgacc ggcgtcgcat ccggcactag ccgtaaattc tatacaaaat
    32101 taccgccgct ccagaatctc aaagcaatag ctgtgagagt tctgcgcatc agcatcgtgg
    32161 aattcgctga ataccgattc ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct
    32221 tccacttctg cgtcgatatg cgtcagatac agtttctgcg cttttggcag gaactgctca
    32281 taaacgcgac cgccgccaat caccatgatt tctggtacgt caccacacgc cgcgatggct
    32341 tcatccaccg acttcaccca cgttacgcga tcgtccgtac ccggttgact gctgaggata
    32401 atatttttgc gtcctggcaa cggacgaccg attgattccc aggtatggcg gcccataatc
    32461 acgggtttat ttaaggtgtt gcgtttaaac caggcgagat cggcaggcag gttccacggc
    32521 atggcgtttt ccatgccgat aacgcgatct accgctaacg ccgcaatcag actgatcatt
    32581 gagatttccc gataaaaaaa attgtcgcca ctatacgtaa agcgtaaacc gtcgtcgact
    32641 ggtgcgagga tgatgttgag gaaaatttta tattctgctg gcgagtccac gctctctccc
    32701 tggactcgcc gcattacaat gaaacaaaaa caaacagtta gctgtaaagt gtgatttatg
    32761 tcactcttta ttaggatgag ggtttcgttt ccggttcatc cgccatgttg ccggtatgtt
    32821 taccttcttc ggttccctgc cagccatgtc gttgaattaa tgacagatgt tcgcggtcct
    32881 cggtaatgat ctcacttaac atcgcgctgg tgcgtttata gaccgccgcg cgggctttgg
    32941 tgtcgttctc aaccattgcc atctcttcca ccatctgaat attaaagcgg cggaacacat
    33001 cggcacgttc tcgcgcttca tacggcccca gacctaaact ttccagtgcc agacgcccgg
    33061 ttttcagcgc accttcgaag gtttcacgct ccggcttttc aacgcctgcc tgacgcaaac
    33121 gaatgtagtg gtcgacatcg cgggcgcggg caataatctg caaatgcggg aagtgttctt
    33181 tcaccatctc tgtcagttgc aggttggttt gcggatcgtc gatggcgtta atcagcactt
    33241 ccgctttcgc cgctcccgca gattccagta aatccatccg cgtggcatcg ccataaaaca
    33301 ctttcatacc aaatttacgc aaggtttcga tatggtccgg atcgtgatcg agcaccacca
    33361 ttttcacccc gctggagagc agtaaacgtc cggtgatctg cccaaaacga ccgaatccgg
    33421 caatgattac ccgcggctgt tcttcgtcga tctcatcggc ttcacgcgcc tcttcagtag
    33481 aagattgctc aaggcgattg aggatcacca gcagaatcgg cgttgccgcc atcgacagcg
    33541 ccaccgccag ggtcagcgat ttcgcccact ccggctccag cacattcgcc atctgcgccg
    33601 cgccaaatac cacaaaggca aactcactgc cctgccctaa caacaccgca aaccaacgac
    33661 gctgtttatt tggcacttgc aacggtcggg caatcagcca cagcatggcg attttgatga
    33721 tgaggaaacc gagcagcaaa atgacaatgc gcaatgggtt ttcaagcagc gtgccaaagt
    33781 ctatcgacat gccaacaccg atgaaaaaca gccccaacag caaaccttta aagggttcga
    33841 tatcgctctc cagcgcatga cggtattccg agcttgccag cagtacgccc gccagaaacg
    33901 cgcccatcgc catcgacaag ccgacctctt ccagcagcaa accaaagcca aacacgagga
    33961 ataacgccac ggcactaaac acttcccgca agccagagcg ggctacaaaa cgcagcgccg
    34021 gacgcgtgac atagcgcccc agcaatacca ccagcaccag cgcacccgcc acttttaacg
    34081 ccgagagagc aaatgcgccc atcgtcgtcg aggcactgct cgttgccagt agcggaatca
    34141 tcgccaccag cgggatcgcc gcgatatcct ggaacagcag caccgcaaag gcactgcgac
    34201 ccatttgcgt caccatcaga ttgcgttcat tcatcgcctg catggcaatc gccgtagagg
    34261 agagcgccag cgtcatgccg atcaattccg cgacctgcca gcgcagccca agtaacatgc
    34321 agaacagccc cagcaggccg ccgcaaatca ccatctgcaa tgcgccaccg ccgaacactg
    34381 ccgcacgcag cttccacagc ctttgtggat cgagttcgag gccgataata aacagcatca
    34441 gcaccacccc aatctcggca aagtgcagaa tagattcggc atcggtcacc agtcgcagcc
    34501 cccacgggcc aataatgcag ccggcgatca ggtagccaag taccgatccc agaccaagac
    34561 gtaccgcaat gggtacaatc agcgctgccg aaccgagata aatcagcgcc tgaatcagcg
    34621 tatggctatc catgatgggc ctcctgccat tccagcagac gttgcttata gtgacgcgcc
    34681 tgcccttcga gggtttcgtc gtcacaaata aaggtgcagt gcatggcaaa cggtggcagc
    34741 cagttcagcc cgcagtagat tgccgtcgcc tgtagcggct gcgacagcac atcaaagccc
    34801 ggatgcgcac caatttcaaa atggctttcc ccgccgccgg tcgtcaccgc ccacagcaaa
    34861 tgtttgccat gcagcgcctt gccgccgtga ccgtaagccc agccgtgcga caaaacttta
    34921 tcgatccaaa gtttaagaag cggaggaatg ctgtaccact gcatcggatg ctgccagacg
    34981 atcagatcgg cgcgagacag cgcctcctgc tcggcggcaa tatcgatatt gaagtcagga
    35041 tagagttgat aaagagagcg aatttcgacg ccttccagcg tccttgcctg ttcaagcatc
    35101 cgtttattcg catgggaatg atgcggatac ggatgcgcat aaattataag aatcataggt
    35161 agcccgcgtg attacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    35221 tagtcaataa tattgattca gaaaagagag aaatctgaac aatttacccc attctctgcc
    35281 cgcggatcgt catattgctg gtttgcgcca atgacatccc tcgatgtctc cggggcaaac
    35341 gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg
    35401 ataccgtaat tattgataaa gatcggcagt gcccaggtcg aaacaatggt gccaatacgg
    35461 cttaaggaca taatcacgcc cacggcagag gcgcggatat ctgtcgggaa gagttcatta
    35521 ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc
    35581 atcactacca gccagatccc catatccggg atcagcccca aaaccgccag ctccagcgtc
    35641 atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttaa ccacagcatc
    35701 ggcggaatac agccgagcat aaagaacagg ctaatcacca cgttccccag cgccgcgttt
    35761 ttgccaacac ccaatcccaa caaaccaacg atttgcgggc caaaggtgta aatggcgaac
    35821 attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg
    35881 cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt
    35941 tcgccaaaca gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac
    36001 cagcggggag attcaggcag ttcgaatcga ccaatcaaaa tcaacaaaca ggggatcgcc
    36061 gcgctaccca gcatccagcg ccagccgcct tccacatcat aaagccagta gccgaccag
//
LOCUS       DAJTOR010000019        35905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTOR010000019 DAJTOR010000000
VERSION     DAJTOR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.019"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..592)
                     /gene="wcaD"
                     /locus_tag="OO249_000858"
     CDS             complement(<1..592)
                     /gene="wcaD"
                     /locus_tag="OO249_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5635247.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIK"
     gene            complement(567..1784)
                     /gene="wcaC"
                     /locus_tag="OO249_000859"
     CDS             complement(567..1784)
                     /gene="wcaC"
                     /locus_tag="OO249_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5635248.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1781..2253)
                     /gene="wcaB"
                     /locus_tag="OO249_000860"
                     /pseudo
     CDS             complement(1781..2253)
                     /gene="wcaB"
                     /locus_tag="OO249_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
     gene            complement(2256..3095)
                     /gene="wcaA"
                     /locus_tag="OO249_000861"
     CDS             complement(2256..3095)
                     /gene="wcaA"
                     /locus_tag="OO249_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR5635249.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGH"
     gene            complement(3273..5435)
                     /gene="wzc"
                     /locus_tag="OO249_000862"
                     /pseudo
     CDS             complement(3273..5435)
                     /gene="wzc"
                     /locus_tag="OO249_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
     gene            complement(5438..5881)
                     /gene="wzb"
                     /locus_tag="OO249_000863"
     CDS             complement(5438..5881)
                     /gene="wzb"
                     /locus_tag="OO249_000863"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5635250.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSWETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5887..7038)
                     /gene="wza"
                     /locus_tag="OO249_000864"
     CDS             complement(5887..7038)
                     /gene="wza"
                     /locus_tag="OO249_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5635251.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKTYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQVLMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVM
                     ATEFRLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7347..7496
                     /locus_tag="OO249_000865"
     CDS             7347..7496
                     /locus_tag="OO249_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635252.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            7697..9280
                     /locus_tag="OO249_000866"
     CDS             7697..9280
                     /locus_tag="OO249_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5635253.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(9554..11407)
                     /gene="asmA"
                     /locus_tag="OO249_000867"
     CDS             complement(9554..11407)
                     /gene="asmA"
                     /locus_tag="OO249_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5635254.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRNYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEVVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11429..12010)
                     /gene="dcd"
                     /locus_tag="OO249_000868"
     CDS             complement(11429..12010)
                     /gene="dcd"
                     /locus_tag="OO249_000868"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5635255.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12102..12743)
                     /gene="udk"
                     /locus_tag="OO249_000869"
     CDS             complement(12102..12743)
                     /gene="udk"
                     /locus_tag="OO249_000869"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5635256.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHNLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13061..16358
                     /locus_tag="OO249_000870"
                     /pseudo
     CDS             13061..16358
                     /locus_tag="OO249_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(16397..17254)
                     /gene="alkA"
                     /locus_tag="OO249_000871"
     CDS             complement(16397..17254)
                     /gene="alkA"
                     /locus_tag="OO249_000871"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR5635257.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            17388..18740
                     /gene="yegD"
                     /locus_tag="OO249_000872"
     CDS             17388..18740
                     /gene="yegD"
                     /locus_tag="OO249_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5635258.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSNAAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(18752..20674)
                     /gene="yegI"
                     /locus_tag="OO249_000873"
     CDS             complement(18752..20674)
                     /gene="yegI"
                     /locus_tag="OO249_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR5635259.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPASKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINVNGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FVDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALYDTARERQKQ
                     KFLEGFFASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKASC
                     DRRFVFRPNEAVTPADRQAVMAAKRHRLESALTVGATELQRFRLYAPARTMPLMEPLR
                     QAAEKLAQAQADLSRC"
     gene            complement(20671..21432)
                     /gene="pphC"
                     /locus_tag="OO249_000874"
     CDS             complement(20671..21432)
                     /gene="pphC"
                     /locus_tag="OO249_000874"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCR5635260.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASVTQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(21429..22088)
                     /locus_tag="OO249_000875"
     CDS             complement(21429..22088)
                     /locus_tag="OO249_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5635261.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(22309..22368)
                     /gene="ibsA"
                     /locus_tag="OO249_000876"
     CDS             complement(22309..22368)
                     /gene="ibsA"
                     /locus_tag="OO249_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR5635262.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(22641..22697)
                     /locus_tag="OO249_000877"
     CDS             complement(22641..22697)
                     /locus_tag="OO249_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5635263.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(22969..23025)
                     /locus_tag="OO249_000878"
     CDS             complement(22969..23025)
                     /locus_tag="OO249_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5635264.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            23304..24551
                     /gene="mdtA"
                     /locus_tag="OO249_000879"
     CDS             23304..24551
                     /gene="mdtA"
                     /locus_tag="OO249_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR5635265.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGMIKVKARFNNQNDVLFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGNRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            24551..27673
                     /gene="mdtB"
                     /locus_tag="OO249_000880"
     CDS             24551..27673
                     /gene="mdtB"
                     /locus_tag="OO249_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR5635266.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLIFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYSAFGQRLISTIYTQANQYRVVLEHNTEITPGLATLDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            27674..30751
                     /gene="mdtC"
                     /locus_tag="OO249_000881"
     CDS             27674..30751
                     /gene="mdtC"
                     /locus_tag="OO249_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5635267.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGKLYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            30752..32168
                     /locus_tag="OO249_000882"
                     /pseudo
     CDS             30752..32168
                     /locus_tag="OO249_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            32165..33568
                     /gene="baeS"
                     /locus_tag="OO249_000883"
     CDS             32165..33568
                     /gene="baeS"
                     /locus_tag="OO249_000883"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5635268.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            33565..34287
                     /gene="baeR"
                     /locus_tag="OO249_000884"
     CDS             33565..34287
                     /gene="baeR"
                     /locus_tag="OO249_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5635269.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            34478..34810
                     /locus_tag="OO249_000885"
     CDS             34478..34810
                     /locus_tag="OO249_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5635270.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            34933..>35905
                     /locus_tag="OO249_000886"
     CDS             34933..>35905
                     /locus_tag="OO249_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCR5635271.1"
                     /translation="MLKGLRTLFSRRVLFNVEMNNSVDCELLLIQQQLQALIAEPGNY
                     LAHDCDLNDTLVLCQLTHHHNGYIREKAVQYLGHKEDVAAIEELLLAANDWVKEVRLA
                     ATIALRHLMKDKNAAAFAVNLPMIRNLLQCQRYDHRAFVDEILRYLLTENNRPQLTYW
                     LTCPDKKLSRAVLQTLIEYGYFNDESSLLSILDKPDEGLRMLATTHWLRQQLPLSEAV
                     LTRLLKDRWPRIRQATLFSLTDRAIEMPPALHSTLLLDNNMLIRLRAKNMLHEVMDVP
                     QFWRHVVTSAEYTPSQRRAALYGLDSIHDPNILKLAEWGLSQNVFPLR"
BASE COUNT         8301 a         9514 c         9380 g         8710 t
ORIGIN      
        1 ttttgatacc aaactgtttg atgctgagcc aaattgagat taatgccaga gcgaaaaatg
       61 ccggttcgaa ataaagtgct gtcgtgcgct tgccgccgaa tttaatgaaa ttcagcacat
      121 agctgttgct gtaaatcaga tatttcgaaa tcgactccat aatactgctg ccaccggtga
      181 ggataatttg cgccatctcc actgccgcca gcgccaccac cagccctacc accagataaa
      241 agaaacgtaa tatcttgcga tggttgtgtg gcgaaattgt tttaaaacga atactccaca
      301 ccatgccaat aatgatcaca atatagacaa acagcatggt tgaagtgacg tatttactgg
      361 catccagcga ctgaccaaac aggtagttaa acgccgtgag tcccgcacca atccctaagg
      421 caatcatcaa ttttttaacg ctgatgcgtt ctaaaaacag cagtaacaag acaggtaaaa
      481 aagtgacgat agtgatgggg aaactttcac caagctgggc gattttgacg ttaaccagca
      541 ggtagatcag cggcagcagc aggtagctac agattctgat agaagttgac atactcctcc
      601 agcatctgtt gtccactgta ggcggcgcgg ctgcgctggc tgaacccggc caacgtggta
      661 ccaaatatcg cctgcgcgat ttccggtttg cttaattgca ctagttgcag cacctcttct
      721 tcgctgacgg ttttaccgcc ggatttttgc aacacttccc gcgccgcgtc gctatgggtg
      781 gcaatcaccg gcacgccaat cgatagcgcc tcacacaaaa tcagcgggta gttatcgacg
      841 cgagaactga acaccagcgc atccatctga ttgagcgcgc tcatcaactt gcgcttgtca
      901 gtttcaaagc cgtgattaac cacgttgcca gcggtgaacg gcgagaactt accaaaggta
      961 tgcagttcga ttttgtcgcc cagcgccatc atctcacgca ccagttgctg gttagtcttg
     1021 ccgtcgtaac gcaagtcatg cgccaccacc gcgattttcg gcttgccctg ggtttcgcgc
     1081 accggaggca agtccgccag aatcgcttcg gttgccatat caataccatt attgataatc
     1141 cggcaacgcc ctggaccgta caggctattg aaagcgtcag ccacatgctg gctgggggaa
     1201 ataaactgac agcccagcgc cagcatctca cggaataact ggcgtttgcc cgccaccagc
     1261 tggtgtgcgc gatcaatctt caccggcgga tagttattca aggtcgggca tttctggcag
     1321 cccgttttcc agccttcgca accgtcggta aaggcgcagc gtccggtaac actccagtgg
     1381 tcgtgcagcg tccagaccag agtgacgtcc ggtttatggt ttttcacttt ttcgcaaaag
     1441 cgcaccacgc tcttaagatt tagccagtag ctgtgcagca catgaaaatg caggaccacc
     1501 ggacccggtg tgcgagtaat ggtgcgatat aactcattga aattgccaaa cagatcgcga
     1561 ttaaacagac gaaacagggc aatattcgcc atcgcggtca tccgcggcgt atgtttgatg
     1621 acctgcggat agttctgatg gctgacgctc tctttgccgc ctttgccgta accgtagaca
     1681 aaatgtgacg ccagcccctg ttgcagcgca cgctggtgga gatctaacgc cacacctgct
     1741 gccccgcctt ccgccagtcg cacattaaat tgcagaatat tcatttaatt accttcactc
     1801 gcgctttttc tcctaccacc agcgcgttgt ccgggacaga gtcgagcacc acgctgcccg
     1861 cacccacggt gacgttgtta ccaagcgtga tatcaccaag aataatgacg ttggcaccga
     1921 gttcgacgcc gttgccaatg tgtggacatg ccatgttatc ggcaccacga ttgccgatag
     1981 tgacgccgtg gcgaatggtg aaatcatccc ccgctaccac gtttttattg atcactacgg
     2041 cgtaaccgtg atggatagta aagcggcggc ctggatttca taaccgaaaa agcattcggt
     2101 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2161 gcgccacacc gaacaaaaat gagcaacacg ataggcaaga accatgcagc acgggcgtaa
     2221 actccagctg ttggcacgca gatcttccag catctttagt gcccccgaat accatcagcc
     2281 agacgtttgc cgttacgcac cgacagcagc gtcagcaaag tgcgccaggt catacgttta
     2341 ttgcggatct ggtagagggt aaacagctga tattttttgc ttgcacggtc aaatttatct
     2401 ttgtgctttc ggtaaaagtg gaagtacccg gagaattttt tcggtgacga ggtgatctgc
     2461 atttcaccgt gattgatatg caggatctgc gtcgcttctt ccactttcca cggttcgccg
     2521 tactccacca ccatccgcag gaagatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2581 aacaaacact ctttgaaacg ccatgcccaa gtaaagacct gattgccaat gatattgcgt
     2641 ttatagaaca ggcggcgtga atacggcgat ttgggatata gcggcaaact tgctggttgg
     2701 gaatagactt cgccctggca aacgtagtcg ttagcgtaca aaaaggcgtg agtgaccagt
     2761 tgctgtttat gggcgaggaa gacgctcaga cggttgggtg tccattcgtc atcgtcatca
     2821 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgtac cgcgcacgcc
     2881 ccgctgttaa tatcgttgtg gatgtaagtg atacgcggat cgttgagggc ggtgacgtac
     2941 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3001 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtc
     3061 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg agtgtcgaga
     3121 tgttctgccg gtttataagc ttgtttacgg gttctgttgc cggatgcggc gtgagcacct
     3181 tatccggcct acggagctgg tttgcttgcc ggatgcggcg tgaaggtagg tcggataaga
     3241 tgcgtcagca tcgcatccga cagcggatgt cgttatttcg catccgactt atattcgtat
     3301 tcgtaatagc cataatcctg atacgcgctg gcgcggcgga agatggagtt cagaatcacc
     3361 cctttcaccg gaataccgtt ttgctcaaag cggctcagac tggtttccac ttctttcaag
     3421 gtgttgaccg cataacgcgc caccattaac gtggttccga catgacgacc aacaattgcc
     3481 gcatcggtca ctgccagaat cggcggcgta tcaatcaaca ccaggtcata gtttttactc
     3541 gcccagttca ccagttcggc aaagcgttcg ctcatcaaca gttcagaagg atttggcggc
     3601 acctgaccgc gcgggatcag gtcaaatttg gcaatagagg tcggtttagc agcggtagta
     3661 atatcgccct gaccaatcag aatttccgac aggccattaa cgttattagt gcacagcagc
     3721 tcgtgggtgt agcctttgcg catatcgcag tcgatcaaca acacccgttt attggtctgg
     3781 ctgatcaccg ccgccaggtt ggcgcagaca aaggttttac cgattgacgg gctaaccccg
     3841 gtcatcatca acacattgtt ctgtgcctgc atcatcgcga agtgcaaact ggtacgcaga
     3901 ctgcggatgg cttcaatcgc cagatcggtt ggattcccca tcgccagtag ctggctctgt
     3961 ttatagcgtt taatcccttt gatggttttg acgctatcgc gcgctttctg ccattccgac
     4021 agcgggatgc tggcatagac gctgataccg tgttcttcca gcacctgcgg gctttcgatg
     4081 ccgcgattaa acaacgagcg cagcagcaca cccacgatag aaagcatcag gccaaggata
     4141 atcgcgccga ggataatcag ccctttcttc ggttttagca cgccaggctg ggtgattgcc
     4201 gggtcaacaa tgcgcacatc gccgacggtg ctggcttcgg tgattttcag ctcctgctct
     4261 ttattcagca gttgcatata gacctgctga ccagactcga catcgcgggt caggcgcaca
     4321 atctcctgct gggttttcgg catcgccgtt acgcgaccgt taagtttggc tttctcttct
     4381 tccagcgcct gacgtttctc cagcagcgtg cggtacgccg ggtgaacttt ggtgtacagc
     4441 ttggagattt ccgcctcttt aaaggtcagt tcgttcaact gcgcgtcgat gttcaccatc
     4501 gaatcgagca ccgctttcgc ttccagcggc agatcaacag aatctttatc ctgacggaag
     4561 gcattcagtt tgttttcggc aacatcgagg cggctacgta cttccggtaa ctgttgcgcg
     4621 aggaaagcga ggcttttcga cgcttccgcc gatttgcgct caatattttg ttcctgatag
     4681 ttacgggcga tgctgttaag aatgtcgcgg atctgttcgc gatcttcacc ggtataagtc
     4741 aggctcagta cgcctgcgtc tttgccgttc tccgttacca tcaggctgtt ttgcagttgg
     4801 ttgatcatcc ccaacgtgga gtatttggtg acggtaaact cactgcccgg gcgggcgtga
     4861 atggcttcaa ccatcagcgt gacgccttct tttttcagca tctggcccgc ttgcccacgg
     4921 gcgctaaagc cgccatcact gctcagggtg tagtttttat tgtccagcac attaagcgta
     4981 aacacctagt ccgccatctc tttcgggcgg ttaaaggtag tcactttcac cgtctcgttc
     5041 tggcgtccca tcagtcgatc ccagcccgcc ccgaaaatcg ggaacgtgtt tttgctcact
     5101 gcaatatcga ggtcgagatc gtccaccgtt ttaccaagca ccagacgcga acgaatcaac
     5161 tggatctcgg cgtccgatgc aggcggtttg ttggctaatg ccgaaccaat gtcctgcact
     5221 aacgaattgc cgctgttttg ctcgatttgt accagtgcgt cggcactata aatcggcgtg
     5281 gcgaagaagg tgtaaaccac ggcacagagg acaaatacag cggtgatgcc aatcacccac
     5341 cagcgcgctt caatgacggt gccgaccagg cgaccaatat cgatttcatc actgcccgtt
     5401 accggagcgg catgttgttt tactttttct gtcattctta tacctgctct gcgttcaatg
     5461 cctgcgccca ctggcgggca gaccgttcaa gtaatgtgta caccgctgca aacgtttccc
     5521 agcttttgcg atacggatcg gggatttcac attcgttatc ccagtgacca aacagcatca
     5581 ctttgccgcg catctcaggt gccatctcgc ataagcgttc gatatggcgc ttttccatgg
     5641 tcaaaatcag gtcatagttg cgacacaggc ggcggctgat ttgtcgtgca cagtggcctt
     5701 ccagagacag ttgatgttct gcggcgacgc tgatagcggt aggatcagcg cccttaccga
     5761 ccagcgcgcc gagtccagcg gactccactt tcagttccgg gtgataacgt tgcagtaagc
     5821 gttccgccgt cggggaacgg caaatattgc cgacacagac aactaatatg ttgttaaaca
     5881 tgacgattac cagttatgaa tgtcgctggc tgtatccgtc atgtaacgga caccgctaat
     5941 agttggcagc aactgattga tcagacggtt ccagcgggaa accggggcgg tggtgacata
     6001 caccacgtca taaggttgca ggcggaattc tgtcgccatc accagcgacg tggcatcgga
     6061 catatccagc tggtagatat tggcaatctt gccgttacgc ccgccctcgc ctttcagcgg
     6121 acgaatgaca aagatgccgc tggcgttgga ggtggtcatg tcgatacctt cagcattacc
     6181 cagggcttca gtcagggctt cagtcagggt catgccgcta aagtccattt tcagggtgct
     6241 ctgtttcttc acttcaccca tcacaaatac tttcagatca tcattacgcg gcacgtagag
     6301 aatatcgccg ggataaagca gtcggttctg attgaggtcg ccgttttgca tcagcacctg
     6361 caaagaaatg cgttcttcac gaccattgtg tgttagcacc acgttgcgcc agtcagcggt
     6421 gtcggtcagg ccacctgcgg cgttgatggc gtcgagaatg gtcagtggta cgttggtgat
     6481 cgcctgttga ccggatttat tcacctgacc tgagatatag gtcttttgtg agcggaaggc
     6541 ggcgatatta acgtccacct gcgggtcagc gatgtacgtc gctaagcgcc cggtaatatc
     6601 actgcggatt tcagcgagcg ttttcccgac tacgtgaacc ttgccgatat acgggtaaaa
     6661 catagtgccg tcaggctgta cccagttgcc ggtgtcgctg gagctgcggt actgaccagc
     6721 tggcgtggtg agttccgggt gatcccagac ggtgacatta agaacgtccc ccggcccgac
     6781 gcgatactgg taattcgcga tctcgctttc cagcgtcata ttggggcgcg ctacattcgg
     6841 gcgtgggcgt aattggtcaa tcaggcgcgg ggtcagcgga taaacattca ccattttgtc
     6901 gagatcgaaa tcagcgtcct gctgtttgat gacgtctttg cccatcgtcg acatattgct
     6961 gcccggaagt actgtgcaac cgcttatcaa ggttactgac accaataatg gcatcaattt
     7021 cattttggat ttcatcattg tttatttatc actttggcag agtaattatc ctgtgcacta
     7081 ttaatagcaa tgtcgccatg cacatttacc ttgcagttaa ttgaatacaa atttaactgg
     7141 catcagtcct aaaaaaattg atttcatccg caggctattg acagaataat tcagactggt
     7201 ctttcaggca tccagacacg ctaccgcccc tggcttttta gccaccaata cactgattta
     7261 gcttaatttt ttaaaccctc tctgcataca atcgttgatg agaaagggtt attgcggaaa
     7321 ttaacttccg aatataaggt gacattatgg taattgaata ttggctctcc aataatgcag
     7381 gaggaagtgt tacagctaac ggaatagcag gcaaggtaac aattcggcaa ttggctattt
     7441 ttaagaatta tattaagtgt cattcaatat ggtttttagg agtttcttta ggttgacaat
     7501 atttaatatt gcgcctccgc atgcgatatt tcttaaataa tgttttatta ttaccacttt
     7561 taattcaggg ataatgtgag gttattaccc tcaaataata tgagataaat agtgctgcaa
     7621 cattgcattt ttgccccgat ttatccatga tcgaattgtg acatttgtca tacaacgaat
     7681 aggttttgta cttactatgg aatggattgc cgatccgtct atctgggccg ggttaatcac
     7741 gctgattgtg atcgaactgg tcctcggcat tgataacctg gtctttattg ccatcctcgc
     7801 cgaaaaacta ccgccgaagc agcgcgaccg cgcacgggtt accgggctgt tgctggcgat
     7861 gttaatgcgc ctgttactgc tggcgtcaat ctcctggctg gtcaccctga cgcaaccgct
     7921 gttcagcttc cgctcgttta cctttagcgc ccgcgactta atcatgctgt ttggtggttt
     7981 cttcctgttg ttcaaagcca cgatggagct gaacgaacgg ctggaaggga aagacagcaa
     8041 taatcccaca caacgcaaag gtgcgaagtt ctggggggtg gtgacacaaa ttgtggtact
     8101 ggacgccatc ttctcactcg actcagtgat taccgccgtc gggatggtcg accatttact
     8161 ggtcatgatg gccgccgtgg ttatcgctat cagcctgatg ttgatggcca gcaagccgtt
     8221 aacgcaattt gttaacagtc acccgaccat cgttattctc tgcttaagct tcctgttgat
     8281 gattggcttt agcctggtgg cagaaggttt cggcttcgtc attccgaaag gctacctgta
     8341 cgctgccatt ggtttctcgg taatgatcga ggcgctcaat cagttggcta tctttaaccg
     8401 gcgacgtttt ctttccgcta accagacgct acgccagcgg actaccgaag cggtaatgcg
     8461 cctgcttagc gggcaaaaag aagatgcgga actggacgcc gaaaccgcgt ccatgctggt
     8521 ggatcatggt aaccagcaga tctttaatcc gcaggaacgg cggatgattg agcgggtact
     8581 taatcttaac cagcgtactg tcagcagcat tatgacgtcg cgccacgata ttgagcatat
     8641 cgatctcaac gcgccggaag acgagatccg ccagttgctg gagcgaaatc agcatacgcg
     8701 gctggtggtt accgacggtg atgacgcaga agatttgctc ggtgttgttc acgttatcga
     8761 cctgctacaa cagtcactgc gtggcgaacc gctcaacctg cgggtgttga ttcgccagcc
     8821 gctagtgttc ccggaaacct tgccgttgtt acctgccctg gagcagttcc gtaatgcccg
     8881 cacacacttt gcttttgtgg tggatgagtt tggctcggtg gaagggattg tgacattaag
     8941 tgacgtcact gaaaccattg ccggtaactt accgaacgaa gtggaagaga tcgacgcccg
     9001 ccatgatatt cagaagaatg cggacggttc ctggacggcg aatggtcata tgccgctgga
     9061 agatctggtg caatatgtgc cgctgccgct ggatgaaaaa cgtgaatatc acaccattgc
     9121 cgggctgttg atggaatatt tacagcgtat tccaaaacct ggcgaagaag ttcaggtggg
     9181 ggattatttg cttaaaacgt tgcaggtgga aagccatcgc gtacagaagg tgcagattat
     9241 tccgctgcat aaggatggcg agttggagta cgaggtgtga tggtatcggt cattttaccc
     9301 aatagccgaa aggaataaga tgagggaacc gcgccagcgg cgatttaccg ccgggagccc
     9361 gggttgccag ggtggtggcg attgagcccc cctggcacgt tcaccggctt gagcgttcca
     9421 tagtagcgag gaacatcaag tgaacggaac agccttcgat cttacatatt cagaatataa
     9481 tgatgccgcc acgaacgcct gatgcgacgc tttacgcgtc ttatcaggcc tacaggacag
     9541 gtcaccaggt aacctacatc ttctccagca acttcttcac atctttgcca ttgcgggaat
     9601 ctttattccg ctcggcccag tcattcagac gacgtttcgc ttcgtcctgt agatgtttgc
     9661 gcagtaactg atccacttgc agactgtaat tgagttgctg ccagttgcca taaacccgca
     9721 gcggtactgg cgtttcttta aggaaatcaa tcagtttgct ttccccgttc cagccaccca
     9781 cgacccgaat atcaaactgg gtgtcgcagg tttgctccgc cagattcaac gtaccagccc
     9841 cggaaagcgc cagcacaggc gattgacctt gcatgtcgtt taacgtcacg acgccatcct
     9901 tcaacgtcaa atcggtggta aagcggtcaa gacgcgttac gttatcgaag ttttcagcgg
     9961 ccttcacatc accaccatta cgttctaccg cttgctgaat catctgctgg aagttcatcc
    10021 cttccatgcg cgtgtcggtc atttcgacat gtgcctgtcc ttgccagttg tggcggaatg
    10081 cgtcggcatc tatgtcagca ccggagaaat caccagccag tgacatcttt ccggtcaacg
    10141 aaatcggata gttaaacgcc ttcagaatgg tgccaatctc aacgttttcc agccgtggct
    10201 ggaagtttat ccgcggattt attgatgtcg cgtccagcgt gcccggcagt gaaacctgtc
    10261 caccgttaag cttgccctgc agttgagtaa tttccagcaa acccgacttg ttggtcattt
    10321 gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac gttactggcc tgcaacaaaa
    10381 tatcagccgt aaagccttgc agtccctgat atgccggttc atcaatacgc gaagaaatga
    10441 ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc ggcaccgttt tcaccattcg
    10501 ctgtttcatt aagcgggatg aggttgtcaa gattcagttg cgggaattgc agtctcagct
    10561 gccattccgg tttctctgtc agcgtgacct gtgcttgccc gctcagcgta ctgtcattgg
    10621 cggtcaaact aatttggtta aatgaaaggc gtttatgtga ctcctgccac tgggcctgga
    10681 aactcccctg cccatgaata ccttgttttg gtaaatctgc accctgcaac tgccagttaa
    10741 tttgttcaat agccgccgtt aaatcatgcg gataatcaga agcatctacc gtaccgttaa
    10801 gggatatcgt gagatcgcgc tgatcgcgat taacccgccc ggagaactca aatgaaccac
    10861 gatgttgggg atcttgttcc atttgcaggc gaatattgcg gattgtcacc tgctcgtcat
    10921 cttcatgctg gaacaccagc acgctgtccg ccaccttaag actggatata tcaaacgacc
    10981 atccgcgatc gtctgacaga tccggcaagg tattgtcgcg cggtgcaacc ggagcgtctt
    11041 cactgcgcac cacttccgtc tgcggcgtca gttggatcac tgcccctttt agcatcacct
    11101 gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc cacgtccaga cgcatgttgt
    11161 cggcgcgaac cagtggctgg ctcgcgccct gggcggtgag agacattcgc ccagagagga
    11221 tactaagctg cggccagacg tgccaacgca gtggcccgtc gagctgcaat tgataaccgc
    11281 tacgtgcagc aacttgcttg accatatagt tgcggaaatc attcggattc accagcaaca
    11341 ctaacgcaga taacccggcc accagcacga ccaggagtat catcagcgtc gtcagaaatc
    11401 gtctcatggt atcctcaatg ggcctgaatt agtctttatc gattcggctg gctaccgcgc
    11461 cctgctggtt gcgatatttc gcatcttcac ggcggttgta aggtcgcgcc gccgggccgg
    11521 aaagcggctc aaagctcagc gcaccaatta acatgcccgg acgcagcgcc agcggcagct
    11581 taccggagtt gtagaactcc agcacaatgc aaccagacca gcccggatcg atacggtgcg
    11641 cggtgacgtg caccatcagc cccagacgcg ccaaagagga acgcccgtcc agccagccca
    11701 ccagatcggc tggcagcgtc accgactcca gcgtcaccgc cagcgccagt tctcctgggt
    11761 gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc gctcatcacg cggtcaagcg
    11821 cggcgctcac ttcatctttg ggaccgctca gatcgataaa cgctgccgtg tgaccacgga
    11881 aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc gccgttaata cgctccactg
    11941 gcggacgtgg gttgatcgac aaacggcctt catcaagcca ggcttcaata tctcggtcac
    12001 acagacgcat ggcattttct cctttcgcgc atcactccct taacgccaat tacgtcaagg
    12061 gcataccagg ttatcactga acggtacaca atttatcaag cttattcaaa gaactgactt
    12121 attttcgctt tcaatatatc gatcgcgatg cggtttttcc cgccgcgcgg cacgataatg
    12181 tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca tcgggcgcac ggttttttga
    12241 tattgcgcca tcacggaatc cattgaacgc ccacgctcgt taacgtcacg cttgatgcgg
    12301 cgcatcaggc agatatccag cggggtatca acgaaaatgg agaagttaag ttcgtcacgc
    12361 aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga tgaccttctt cggctcaacc
    12421 gtcaccgttt ctttcatacg cgtatgttca acatagctgt aaaccggcag gtcaattgcc
    12481 gagccgcgtt tcaacgcttg taaatgctca agcagcaggt tgtgatccat cgcgctggga
    12541 tggtcgtagt tggtcttaac gcgttcttcc atcgacagat ggctttgatc tttgtaatag
    12601 cagtcttcgg gaattacgcc gatgtgttca tcaccgactt gctcacgtaa ttcacgataa
    12661 agggtactgg caataagact cttgccggaa gccgatgcgc cagcgatacc gataatgacg
    12721 cactgatgag actgatcagt catatattta gcgacctgat taacctggat gttaggaagg
    12781 gggcgacgaa gcgccaaacg cggcaattat agggatttca tccgcctgat accagtcgaa
    12841 tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa ttcccggtgg caaattacgt
    12901 tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt gcgcaaatta tccgccttta
    12961 cgtcacttta tgagcaattc acatataaaa tgtaaaactt ttgtactagc ataaacacag
    13021 aaacgaatac tggcgacctg gtcttgcgga taaagcggta atgagcaaac aatcacagca
    13081 tgtattaatt gccctgcccc acccactgct tcacctggtc agtttaggtt tagtctcgtt
    13141 tatctttacc cttttctcgc ttgagctttc gcagtttggc acccaactcg ccccactgtg
    13201 gttcccgacg tccatcatga tggtggcgtt ttatcgccat gccgggcgca tgtggccggg
    13261 aattgcgctg agctgctcgc tgggaaatat cgccgcatcc atcctgcttt tttccaccag
    13321 ctcgctgaac atgacctgga cgaccatcaa tattgttgaa gccgtggtcg gggcagtgct
    13381 gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat ctggctgact ggctgcgtct
    13441 ggcattcggc agcgccattg ttccgcctct gttagggggt gttctggttg tcctgctgac
    13501 gcccggagac gatcctctca gggcattttt gatatgggta ctgtcagaat ccatcggcgc
    13561 tctggtactg gtgccgctgg gattgttatt taagccacac tatctgctgc gccatcgcaa
    13621 cccacggtta ctttttgagt cactgctcac attagccatc acgctgacgt taagctggct
    13681 ttctatgttg tacctgccgt ggccttttac tttcattatt gtgctgttga tgtggagcgc
    13741 cgtgcgcctg ccacgaatgg aagccttttt gatcttcctt accacggtga tgatggtgtc
    13801 gctgatgatg gccgcggatc cctccctgct tgctacgccg cgtacgtacc tgatgagcca
    13861 tatgccgtgg ctactgtttt tgctgatcct gctgcccgcc aacatcatga ccatggtgat
    13921 gtatgccttt cgtgcggaac gcaaacacat ttccgaaagc gaaacccgct ttcggaacgc
    13981 gatggaatat tccgctatcg gtatggcgtt agtgggcacc gagggacaat ggctgcaaac
    14041 caacaaagcg ctctgccagt ttctcgggta cagtcaggaa gagctgcgcg gactcacctt
    14101 tcagcaactg acctggccgg aggatctcaa taaagatctc caacaggttg aaaagctgat
    14161 aagcggtgaa ataaacacct attcaatgga aaaacgctac tacaaccgta atggcgatgt
    14221 tgtctgggcg ttgcttgccg tctcactggt gcgccacacg gatggcacgc cgctctattt
    14281 tatcgctcag attgaagaca ttaacgagct aaaacgcacc gaacaggtga atcagcaact
    14341 gatggagcgc atcacgctgg ctaacgaagc gggcgggatt ggcatctggg agtgggagct
    14401 gaagccgaat atttttcgag ctgtatgaaa ttcctccgca tatcaaaccg aactggcagg
    14461 tgtggtacga gtgcgtgctg ccggaagatc gccagcatgc cgaaaaagtg attcgtgatt
    14521 cgttgcaatc acgctcgccc tttaagctgg aatttcgcat taccgtaaaa gacggtattc
    14581 gccatatccg cgccctcgcc aaccgggtac tgaataaaga aggcgaagtc gaacgcctgc
    14641 tcggcattaa tatggatatg accgaggtta aacagcttaa cgaggcattg tttcaggaaa
    14701 aagagcgcct gcacattacg ctggattcca tcggtgaagc cgtggtctgt attgatatgg
    14761 cgatgaaaat tacctttatg aatccagtgg cggagaagat gagcggctgg acgcaggaag
    14821 aagcattagg tgttccgctc ctgacggtgt tgcatattac ttttggcgac aacggaccat
    14881 taatggagaa catttacagt gccgacacct cacgttccgc gattgaacaa gatgtggtgt
    14941 tgcactgccg gaacggcggc agttacgacg tgcattacag tattacgccg ttaagtactc
    15001 tggacggcag caatataggc tcggtactgg tgattcagga cgtcaccgaa tcacgcaaaa
    15061 tgctgcgcca gctgagctac agcgcctccc atgatgcact gacgcatctc gccaatcgcg
    15121 ccagttttga gaagcaactg cgtatcctgc tgcaaacggt aaacagtaca catcagcgac
    15181 atgccctggt gtttatcgat cttgatcgct ttaaagcggt gaatgacagc gccgggcatg
    15241 cggcgggtga cgctttactg cgcgaactgg cgtcgttgat gctgagtatg ctgcgctcca
    15301 gcgacgtgct ggcgcgactc ggtggtgatg aatttggtct actgttgcca gactgtaata
    15361 tcgaaagtgc gcgttttatc gctacacgca ttatcagtgc cgtgaatgat tatcacttta
    15421 tatgggaagg ccgtgtgcat cgggtaggtg ccagtgccgg gattaccttg attgatgaca
    15481 acaatcatca ggcggcagaa gtgatgtcgc aggctgatat cgcctgttat gcctccaaaa
    15541 atggtggacg gggccgggtg acggtttacg aaccacagca agctgccgca catagcgaac
    15601 gggcggcgat gtcgcttgat gaacagtggc ggatgattaa agagaatcag ttgatgatga
    15661 tcgcccacgg tgtcgcttcg ccgcggatcc cggaagcgcg taatttgtgg ctgatttcac
    15721 ttaagctctg gagttgcgaa ggcgagatta ttgatgaaca aaaatttcgt cgtagcttca
    15781 gcgatccggc acttagccat gctcttgacc gacgggtatt ccacgaattt ttccagcagg
    15841 ccgcaaaagc ggttgccagt aaaggcataa gcatcgccct ccccctttcc gttgccggtt
    15901 tgagtagcgc cacgctggtg aatgatctgc ttgagcagct gggaaatagc cctctaccac
    15961 cacggttatt acatctgatt attccggctg aagcgatttt agatcacgca gaaagcgtgc
    16021 aaaaactgcg gctggtggga tgtcggatag tgctcagcca ggtgggccgc gatctgcaaa
    16081 tcttcaactc gctgaaagcg aatatggcag attacctgct acttgatggt gagttatgcg
    16141 ccaacgtgca gggtaatttg atggatgaga tgctgattac gattattcag gggcacgctc
    16201 agcgactcgg gatgaaaacc atcgccgggc cagtcgtttt acccttagtg atggatacgc
    16261 tttctggcat cggcgtcgat ctgatttatg gtgatgtgat tgccgatgcc caaccgctgg
    16321 atttgctggt gaatagcagt tatttcgcga ttaactgaga gagcgtttgc ccattgcctg
    16381 atgcgacgta tcaggcctac aattcgtccg ttccgtctgg ttgccagcct tccgtatacc
    16441 agatatgcaa cagcgcataa gaacgccagg gcttccagcg ctcggcataa cggcggattt
    16501 gtgccggtgt catccccgga aatcgctgtt taatcagata atcatccggc agaaaaacat
    16561 ctttcgcctg ccagccacgc aaagcgaaat aattcgccgt ccagcgcccg atacccggaa
    16621 aagtttgcag cgttttcatc gcctgctcca catcgcccgg aattgtcatt ggtaagctgc
    16681 cctccagcgc cgcatttgcc agatgaatca gtgcctctgc ccgtttcaac ggcatgccta
    16741 acgcttttaa tgcctgcggg tcggctgctg ccagccgctg aggcgtgggg aagcagatat
    16801 actccggaaa atcatccagc cgttcgccat aaagctgtac aactttggca gtcaattttg
    16861 ccgccatcgc cacgctcacc agttggccta aaatcgcccg cacaccctgc tcaaaagcat
    16921 caatacagcc gggtaaacgc aatccgggcc gcgccgcgcc taacttgccc aacgcaccgt
    16981 taacaatctg cgggttacat tgcagatcaa acaggcggct cattttcgcc agacactcag
    17041 cggcaacggg ttctaaacct gcacttaaat ttatgtgcag agtatggcgg gctatatccg
    17101 gaatagcagt caccacgccg cgatattcgc ccaccgccag actacgggca taataatcgt
    17161 cagcgaccgt ttccacacca ctcactgcac gggcggcgag aaatcccaac atccacgacc
    17221 agtcatacgg cggctgccag ttcagggtat acatcgcatc tccttattca tccgctttca
    17281 gcataaacgt tattcagacg ctacgctttg ctttcatatt ccggttgtcg cgacggcaac
    17341 atttcgctaa agtcacgccc cttcttcacc ggcatgggga ttatttcgtg tttattggtt
    17401 ttgattacgg tacagcaaac tgttcagtgg cggtcatgcg tgacggtaaa ccgcacttgc
    17461 taaaaatgga aaacgacagc acgctgctgc cttcaatgct ttgcgcgcca acgcgtgaag
    17521 cggtaagcga atggctgtac cgccatcatg acgttccggc agacgacgat gaaacgcagg
    17581 cgttgttacg acgggcgatt cgttataacc gcgaagaaga tatcgatgtt acagcgaaaa
    17641 gcgtgcagtt cggtctttcc tcactggcac agtacattga tgatccagaa gaagtgtggt
    17701 ttgtgaaatc accaaaatcg ttcctcggtg ccagcggctt aaaaccgcag caggtagcgc
    17761 tgtttgagga tctggtctgc gcaatgatgt tgcacattcg ccagcaggcg caggcgcagc
    17821 tgccagaagc gattactcag gcggtgattg gtcgcccgat caacttccag gggctgggcg
    17881 gcgatgaagc aaacgcccag gcgcaaggga ttctggaacg cgcggcgaag cgtgccggat
    17941 tcagggacgt ggtattccag tatgagccgg tcgcggctgg gctggattac gaagccacct
    18001 tgcaggaaga aaaacgggtg ctggtggtgg atatcggtgg tggtacgact gactgttcat
    18061 tgctgctgat gggaccgcag tggcgttcgc gtctcgatcg tgaagccagc ctgctgggtc
    18121 acagtggttg tcgtattggc ggtaacgatc tggatatcgc cctggcgttt aaaaacctga
    18181 tgccactgct gggtatgggt ggcgaaaccg aaaaaggcat cgccctgccg atcctgccgt
    18241 ggtggaatgc ggttgccatc aacgacgtac ctgcgcagag tgatttctac agtagtgcca
    18301 acggtcgtct gcttaacgat ctggtacgcg atgcccgcga accggaaaaa gtggccctgt
    18361 tacagaaagt ctggcgtcag cgtttaagct atcgcctggt acgcagcgca gaagagagca
    18421 aaatcgccct ttcaaacgct gcggaaaccc gcgcctcact gccgtttatc agcgatgaac
    18481 tggctacgct gattagccag caagggctgg aaagcgccct cagccagcca ctggcgcgga
    18541 ttctggaaca ggtgcaactg gcgctggata acgcccagga aaaaccggac gttatatacc
    18601 tgaccggcgg tagcgcccgt tcgccgctga ttaaaaaggc tctggcagaa cagttgccgg
    18661 gcattccgat tgcaggcggc gatgactttg gctccgtcac cgccgggctg gcacgctggg
    18721 cggaagtggt atttcgttaa aagaaaatgg ctcagcagcg gcttaaatct gcctgcgcct
    18781 gagccagttt ttctgccgcc tgacgtaacg gttccatcaa cggcatggtc cgtgctggag
    18841 cataaaggcg gaatcgctgc aactctgtcg cgccgacagt caacgccgat tccagccgat
    18901 gtcgtttggc ggccattacc gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga
    18961 acacaaaacg gcggtcacag ctcgctttcc agtcgataac cgcctgggtc agatggtcgc
    19021 caaacccttt aacttgctta acgccacgac gggtaacatc cgctgctgtt tcaataccaa
    19081 aagaccgtaa cgccgcttta cgcgcagggc caacgccggg aatagaggca aaaaatccct
    19141 ccagaaactt ctgcttctgc cgttcccttg cggtgtcata aagtgctgcc agagcgcgtt
    19201 tctcctcttc cggtaacccg agaatttcgt ccttcatttt ttcgagcatc gcccgtttgg
    19261 cgataaaacc ttccagcccg cccaactgtt ggatctggct gactaaatgc tcatagtcca
    19321 ttttcgcgcg attaaatgcc tcacggcgtt gctggatttc tgctttgtac actttgcttg
    19381 tcagactgcc aataatccag atagccgaca gcacaggaac caaaataata taacgcggtt
    19441 ctgcctgaag gccgcaaagc aacagcgata acgctgacag tgcgatctca atcagaatga
    19501 tgtattcgcg ccgtaacagg cctaaaggaa gcggcctgcc agtcggttgg aaatgatcgg
    19561 gtaatggcaa ttgcaatgct ggcggtgcta ctgacgccat caccatcgcc cagactttcg
    19621 ccagcacaaa atcaccgctg gtggtaatga cctcttcacc gagatcaaca aaatagataa
    19681 cgccttgatt atccagcgta caccacgggc agtcggtcaa atgagcggga taaacatgca
    19741 ttgccgaaac gggacatttc tttaactgtt ggcgcagaga atcaagtgcc gctacccacg
    19801 ctttagccgt cggacgcccg gttgccacgc cactttccgt gaatgcctgc tgaaacatgg
    19861 cttcaacatc actcggtaac atcgatagcg gaatcgatcg tggcggcggt tttaaaccac
    19921 gtcgctgatt atctgacgcg taggcataac ggaaatgggt aatatccgtc tccagcgcat
    19981 tacccgcatc agagataagc ggcacaccag aataaggatg ccggccacca aacaagacgt
    20041 gaaaaatcag caacgcaagg ccaaaattat cgtgattttc tgtgcgttca aagccgacaa
    20101 atgacgacaa ggtttgtagc tctggcggcg taaaatgcga cacgccgact tcgcataaat
    20161 gcagtgtgcc attgacgtta atctgaaagg agtcgctatc aatcagtacc actttgctgt
    20221 cgcgacctac cataaaactg ttctggttta cgtcacccac gacgtgcccg tgctcgtgaa
    20281 ccgtagcaaa agatgaagca atattgcgcg caacatagag tagaaaatcc cacgcacaat
    20341 gagggtaact ctggcgacga tgtgccgggc tatagatcat atgaatcggt tctttaccag
    20401 aaacttttgg catcataaaa ccgataactt ttccgcctcg tccaccgtga agcgttgcct
    20461 gcggccaggc gacataattc aacaactgcg cgtcagctgt cgcagccata aaggcaagtt
    20521 tgtcctgttt tgaggcgggt ggcggcgtgt gataaatctt ggcgacgcta tcgacaaact
    20581 cctcgatatc ataaaccgcg ccttcaccgc ctttgcccag ttcacggcct ggcgtaacgc
    20641 actcaccagt agcagtatat aaagtgggtt tcatggtaac cacagcgcca gtgccaacgt
    20701 tttatcatca tccgtacgct cgtttaccgc cgggctggac aaaaactgct ttaacaattc
    20761 aggtaataaa tcgagttgtt cctgcgttac tgacgccagt ccattgaaaa acggggtaaa
    20821 gaaaggcaca tgaggagaat tatccagcat atttaacgcc agccgctgaa taccatccgt
    20881 aaatgctgca actttatgcg cacgcccagt gctggtgaaa gtttccagtc tggaaacggc
    20941 atcttcgtcg gtaatgaagt gcgtcatgtt ggcatattca cccaccatcg gcgtgagagg
    21001 caattgcaga ccgtgaccca aatctaccac cacgccgcca tcgccaatct gcataatcaa
    21061 ggtgccgtca ggcgatgaaa tcaatcccag aaacgtgcag gcaaaatcgc gaactgctaa
    21121 tgctttggct tctgcctcgg caaaaagtcg ttgacgaata gtcagtacca tatttgtggc
    21181 gaggacatca ttaagcccca attcaccgtc ctgtactttt tgcaccataa acgccatcgc
    21241 ttcattgacg gcgagcgtcg cgccttctcc tccctgcgaa acgctacctg cgccatcagc
    21301 aaggaacatc accagtaatg gttgctggtc gtttaaccag acaacctgca tctggcaggc
    21361 atcctgacaa ggcaaatcag cactaatgtg cgatgttcca acagcggatg cataaaccag
    21421 acgccagctc acactgacgt ccagcctttt ggcgcttcca gcacaacttc cgttcccggc
    21481 gtggagcggg aaaccgaacg cagcgaactg gataaccagc taaacagttc acggaattgt
    21541 aatccctgca gtggcaaagg ctgacgaacg ctgatttgtg ccagagtctt catatccgcc
    21601 ccctgaacac cgatggaaaa gaaggcaaac ttcttatctt cttccccctg aaacactttg
    21661 ttggcggcag cctgccactc atcggttgga gcgccatcgg tgatcaggaa aatccacggg
    21721 cgataatacg aaataccatt tgcgcggtac tcacgtttcc tctcttcaac catatctagg
    21781 gctttggtaa tggcggcccc cataggcgta tccccctggg caaacaggat gggcgggaaa
    21841 aagttagcgg cgctggtaaa gggttgttcc acatgcaccg ggccgaacgt cacaatgccg
    21901 agttccactc tttttaaagc cagcggatca gcaagcagtt catcacgaaa ggtaaccaat
    21961 ccggcgttaa gttcattaat aggtctgccg ctcatcgagc cagaaacatc cagcagcaaa
    22021 atgcaggggc aacgtggttc cgggttactg gcaaaatcgc tggtggcaaa cgtgatttgt
    22081 tcgctcataa gaaatccttt cttagagaat tatcgaggtc cggtatcata ccgattgcaa
    22141 taatgaaata agagcaaacg aagaaacttt cagaattagg cccgtcggcg cagaaaattt
    22201 tgctattcgc ctcgcaagaa cggagagcca tcgattgaca ctaagggcgg agtgacataa
    22261 tttcaggagt gagggttagg gagaggtttc cccctccccc tggtgttctt agtaagcctg
    22321 gaagctaatc actaagagta tcaccagtat gatgacgtgc ttcatcataa ccctttcctt
    22381 attaaaagcc ctcttctccg ggagaggctt tcccgtttca gcgtcccgct gaaatcatcg
    22441 gcttacctcc tttcgccctg aatgcagtct atcgctaacg cgtgaataaa atagtttcct
    22501 ctgtgattat tgcgtaacga ctcgcaaaga atatttgctg gcaggatcgc agactacaaa
    22561 gcctacggat tgacaatctt atcgtgaagg catactttca ggagtgaggg tagagcgggg
    22621 tttcccccgc cctggtagtc ttagtaagcg gggaagctta tgactaagag caccacgatg
    22681 atgagtagct tcatcatgac cctttcctta tttaaggccc cttcctcggg aggggctttc
    22741 ccgtttcagc gtcccgctga aatcgtcggc ttacctcctt tcgccctgta tgcagtctat
    22801 cgctgacttg cggataaaat agtttccagt gtgattattg cgtaacgtct cgcaaagaat
    22861 atttgctggc aggatcgcag actgcaaagc ctgcagattg acaatcttat cgtgaaggca
    22921 tactttcagg agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagctgg
    22981 ccagctaatg actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt
    23041 tatggcccct tcctcgggag gggctttccc gtttcagcgt cccgctgaaa tcggcggctt
    23101 acctcctttc gccatgcaag cagtctatcc ctaacgcgta gataaaatag tttcctgtgt
    23161 tattactgga tgcgtgctcg caaatgtgcc cgtcattcag acaattcccg acagtgtttc
    23221 ataattcctc catttttctc ccttattggc tggctacact agtatcattc cgcgaaacgt
    23281 ttcaggaaga gaaactctta acgatgaaag gcagttataa atcccgttgg gtaatcgtaa
    23341 tcgtggtggt tatcgccgcc atcgccgcat tctggttctg gcaaggccgc aatgactccc
    23401 ggagtgcagc cccaggggcg acgaaacaag cgcagcaatc gccagcgggt ggtcgccgtg
    23461 gtatgcgttc cggcccatta gccccggttc aggcggcgac cgccgtagaa caggcagttc
    23521 cgcgttacct caccgggctt ggcaccatta tcgccgctaa tactgttacg gtgcgcagcc
    23581 gcgtggacgg tcaactgatg gcgttacatt tccaggaagg ccagcaggtc aaagcaggcg
    23641 atttactggc agaaattgac cccagccagt tcaaagttgc attagcacaa acccagggcc
    23701 aactggcaaa agataaagcc acgcttgcta acgcccgccg tgatctggcg cgttatcagc
    23761 aactggcaaa aaccaatctc gtctcccgtc aggagctgga tgcccaacag gcgctggtca
    23821 gtgaaaccga aggcaccatt aaggctgatg aagcaagcgt cgccagcgcg cagttgcaac
    23881 tcgactggag ccgcatcacc gcaccagtcg atggtcgcgt tggtctcaag caggttgatg
    23941 ttggtaacca aatctccagt ggtgatacca ccggaattgt ggtgatcacc cagacgcatc
    24001 ctatcgattt agtctttacc ctgccggaaa gcgatatcgc tactgtagtg caggcgcaaa
    24061 aagccggaaa accgctggtg gtagaagcct gggatcgcac caattcgaag aaattaagtg
    24121 aaggcacgct gttaagcctc gataaccaaa tcgatgccac aaccggtatg attaaagtga
    24181 aagcacgctt taataatcag aatgatgtgc tgtttcccaa tcagtttgtt aacgcgcgca
    24241 tgttagtcga caccgaacaa aacgccgtag tgatcccaac agccgccctg caaatgggca
    24301 atgaaggcca ttttgtctgg gtgctgaata gcgaaaacaa ggtcagcaaa catctggtga
    24361 cgccgggcat tcaggacagt cagaaagtgg tgatccgtgc aggtatttct gcgggcaatc
    24421 gcgtggtgac agacggcatt gatcgcctga ccgaaggggc gaaagtggaa gtggtggaag
    24481 cccagagcgc caccactccg gaagagaaag ccaccagccg cgaatacgcg aaaaaaggag
    24541 cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc gcctgtttat
    24601 tatgcgtcct gtcgccacca cgctgctgat ggtggcgatc ttactcgccg ggattatcgg
    24661 ttatcgcgcc ctgcccgttt cggcgctgcc ggaagtggac tatccgacca ttcaggtggt
    24721 cacgctctac ccaggtgcca gcccggatgt catgacctct gccgttaccg cgccgctcga
    24781 acgccagttc gggcagatgt ctggcctgaa acagatgtcg tcgcaaagtt ccggcggtgc
    24841 gtcagttatc actttgcagt tccagctaac attaccgctc gatgtcgccg agcaggaagt
    24901 gcaggccgcg attaacgctg cgaccaactt gttgccgagc gatctgccta acccgccggt
    24961 ttacagcaaa gtgaacccgg cagatccgcc gatcatgacg ctcgccgtca cctcaaccgc
    25021 catgccgatg acgcaagtgg aagatatggt ggaaacccgc gtcgcgcaga aaatctcgca
    25081 gatttccggc gtcggcctgg tgacgctttc cggcggtcag cgtccggctg ttcgcgtcaa
    25141 acttaacgct caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc gcaccgccat
    25201 taccggcgct aacgttaact cggcaaaagg tagcctcgac ggcccttccc gtgcggtcac
    25261 gctttccgcg aacgaccaga tgcaatccgc cgaagagtat cgccagctaa tcatcgccta
    25321 ccagaacggc gcgccaattc gtctgggcga tgtcgcaact gtagagcaag gtgcagaaaa
    25381 cagctggctc ggcgcgtggg cgaacaaaga acaggccatt gtgatgaatg ttcagcgcca
    25441 gcccggtgct aacattatct ccaccgccga cagcattcgg cagatgctgc cacagctcac
    25501 tgagagtctg ccgaaatcgg tgaaggtgac agtgctttcc gatcgcacca ccaatatccg
    25561 cgcatccgtc gatgatactc agtttgaatt gatgatggct atcgcgctgg tagtcatgat
    25621 tatctacctg tttttgcgca atattccggc gaccatcatt cccggtgttg ctgtaccgct
    25681 gtcgttaatc ggcactttcg cggttatggt gtttctcgat ttttcaatca ataacctgac
    25741 actgatggcg ttaactatcg ccaccggatt cgtggtcgat gacgccatcg tggtgatcga
    25801 aaacatttcc cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc tcaagggcgc
    25861 aggtgaaatc ggctttacca ttatctcgct gatcttctca ctgattgcgg tgttgatccc
    25921 actgctgttt atgggcgata tcgtcgggcg actgttccgc gaatttgcta ttaccctggc
    25981 ggtagcgatt ttgatctcag cggtggtgtc gctgaccctg acaccgatga tgtgcgcgcg
    26041 gatgctcagc caggagtcgt tgcgtaaaca gaaccgcttc tcccgtgcct cggaaaaaat
    26101 gttcgacagg ataatcgccg cctatggtcg tggactggcg aaagtgctga atcatccgtg
    26161 gctgacctta agcgtggcac tcagcacgct gctgcttagc gtgctgctgt gggtgttcat
    26221 tccgaaaggt ttcttcccgg tacaggacaa tggcattatt cagggcactt tgcaggcacc
    26281 gcaatccagc tcctttgcca atatggccca gcgacaacgc caggtcgcgg acgtgatttt
    26341 gcaggatccg gcagtgcaaa gcctgacctc atttgttggc gttgatggca ctaacccgtc
    26401 gctgaatagt gcacgtttac aaatcaacct caaaccgttg gatgaacgtg atgatcgggt
    26461 gcaaaaagtc atcgcccgtc tgcaaacggc ggtagataaa gtgccgggcg tcgatctctt
    26521 cctgcaacca acgcaggatc tgactattga tactcaggtc agccgcaccc agtaccagtt
    26581 taccttgcag gccacctcac tggatgcgct cagtacctgg gtgccacagt tgatggaaaa
    26641 actccagcaa ctgccacagc tttctgatgt ctccagcgac tggcaggaca aagggctggt
    26701 ggcgtatgtc aatgttgatc gcgacagcgc cagccgtctg gggatcagca tggcggatgt
    26761 cgataatgcc ctgtacagcg cgtttggtca gcgactgatt tccactattt atactcaggc
    26821 taaccagtat cgcgtagtgc tggagcacaa caccgaaatt accccgggcc tcgcgacgct
    26881 ggataccatt cgcctgacca gtagcgacgg cggcgtggtg ccgttaagct caattgccaa
    26941 agttgaacag cgttttgcgc cgctttccat caaccatctg gatcagttcc cggtaacgac
    27001 catctccttt aacgtgccgg ataactattc gctgggcgat gcggtgcagg cgattatgga
    27061 caccgaaaag acgctgaatc tgccggtgga tatcaccacg caattccagg gcagcaccct
    27121 cgccttccag tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg tggtggcgat
    27181 gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca ttctctcgac
    27241 gctacccacc gcaggggtcg gcgcgctgct ggcattaatg attgccggta gcgaactgga
    27301 tgtgattgcg attatcggca ttattttgct gatcggtatt gtgaagaaga acgccatcat
    27361 gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc gcgatgcaat
    27421 ctaccaggct tgtctgttgc gttttcgtcc gatcctgatg accaccctgg cggctctgct
    27481 tggcgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc gtccgttagg
    27541 tatcggcatg gtcggcggtc tgattgtcag ccaggtgctg acgctgttca ccacgccggt
    27601 gatttatctg ctgttcgacc gcctggcatt gtggaccaaa agtcgctttg ctcgccatga
    27661 agaggaagcg taagtgaagt tttttgccct cttcatttac cgtccggtgg cgacgatttt
    27721 actgtcggtt gccattaccc tgtgcggcat actgggcttc cgtatgctgc cggtcgcccc
    27781 gctgccgcag gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg gtgcgtcacc
    27841 agaaacaatg gcgtcttccg ttgccacgcc gctggagcgc tcacttgggc gcattgccgg
    27901 agtcagtgaa atgacctcca gcagttcgct cggcagcacg cgtattattt tgcagtttga
    27961 ttttgaccgg gatatcaacg gcgcggcgcg cgatgtgcag gcggcgatca acgctgcaca
    28021 aagtttgctg cccagcggga tgcccagccg tccgacctat cgcaaagcta acccgtcgga
    28081 tgcgccaatt atgatcctca cgctgacatc cgatacttat tcgcagggta aactgtacga
    28141 tttcgcctcg acgcagctgg ctccgacgat ttcgcaaatc gacggtgttg gtgatgtcga
    28201 tgtcggcggc agctcactgc ccgccgtacg cgtcgggctg aatccgcagg cgctgtttaa
    28261 tcagggcgtc tcgctggacg acgtacgcac tgccatcagc aatgccaacg tgcgtaaacc
    28321 gcagggggcg ctggaagatg gcactcaccg ctggcagatc cagaccaatg atgagctaaa
    28381 aaccgccgcc gaatatcagc cgctgattat tcattacaac aacggcggcg cggttcgtct
    28441 gggcgatgtg gcgacagtga ccgactctgt acaggatgta cgtaacgccg ggatgaccaa
    28501 tgccaaaccg gctattttgc tgatgatccg caagctgccg gaagccaata ttatccagac
    28561 ggttgacagc atccgggcaa aattaccgga gttgcaggaa accattccgg cggcgattga
    28621 tctgcaaatt gctcaggatc gctcccccac cattcgcgcc tcgctggaag aagtcgagca
    28681 aacgctgatt atctcggtgg cgctggtgat tctggtggtg tttttattcc tgcgctcggg
    28741 tcgcgcaact attattcccg ccgttgcggt gccggtttcg ctgattggta cgtttgcggc
    28801 gatgtacctg tgcggtttca gtctcaataa tctttcgtta atggcgctca ccatcgctac
    28861 tggtttcgtg gtggatgacg ccatcgtggt gctggaaaac attgcccgtc atctggaagc
    28921 gggaatgaaa ccgttgcaag ccgcactgca aggtactcgc gaagtcggtt ttacggtgct
    28981 gtcgatgagt ctgtcactgg tggcggtgtt cctgccgctg ctgttgatgg gcggattgcc
    29041 gggccgactg ttacgcgaat ttgccgtgac gctttctgtc gccattggta tatcgttgct
    29101 ggtttctctg acattaacgc caatgatgtg tggctggatg ctgaaagcca gcaagccgcg
    29161 cgagcaaaag cgactgcgtg gttttggtcg catgttggta gccctgcaac aaggctacgg
    29221 caagtcactg aaatgggtgc tcaatcatac ccgtctggtg ggcgtggtgc tgcttggcac
    29281 cattgcgctg aatatctggc tgtatatctc gatcccgaaa accttcttcc cggagcagga
    29341 cactggcgtg ttgatgggcg ggattcaggc ggatcagagt atttcgtttc aggcgatgcg
    29401 cggtaagttg caggatttca tgaaaattat ccgtgacgat ccggcagtgg ataatgtcac
    29461 cggctttaca ggcggttcgc gagtgaacag cgggatgatg tttatcaccc tcaagccacg
    29521 cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt ctgcgcgtaa aactggcgaa
    29581 agaaccgggg gcgaatctgt tcctgatggc ggtacaggat attcgcgttg gtgggcgtca
    29641 gtcgaacgcc agctaccagt acacgttgtt atccgacgac ctggcggcac tgcgagaatg
    29701 ggagccgaaa atccgcaaaa aactggcgac gttgccggaa ctggcggacg tgaactccga
    29761 tcagcaggat aacggcgcgg agatgaatct ggtttacgac cgcgacacca tggcacggct
    29821 gggaatcgac gtacaagccg ccaacagtct gttaaataac gccttcggtc agcggcaaat
    29881 ctcgaccatt taccagccga tgaaccagta taaagtggtg atggaagtgg atccgcgcta
    29941 tacccaggac atcagtgcgc tggaaaaaat gttcgttatc aataacgaag gcaaagcgat
    30001 cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc ccactatcgg tgaatcatca
    30061 gggattatcg gcggcctcga ccatttcgtt taacctgccg accggaaaat cgctctcgga
    30121 cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggg gtgccttcga cggtgcgcgg
    30181 cagttttgcc ggcacggcgc aggtgttcca ggagacgatg aactcgcagg tgatcctgat
    30241 tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga gttacgtaca
    30301 tccgctgacg attctctcca ccctgccctc ggcgggcgtt ggagcgctgt tggcgctgga
    30361 gctgttcaat gccccgttca gcctaatcgc cctgataggg atcatgctat taatcggcat
    30421 cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt gaagcccaac ggcacggtaa
    30481 cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc cgattatgat
    30541 gaccaccctg gcggcgctgt ttggtgcgct gccgctggta ttgtcgggcg gcgacggctc
    30601 ggagctgcgg caacccctgg ggatcaccat tgtcggcgga ctggtaatga gccagctcct
    30661 tacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctgcggc tgcgtttttc
    30721 gcgtaaacct aaacaaacgg taaccgagta aatgacagat cttcccgaca gcacccgttg
    30781 gcaattgtgg attgtggctt tcggcttctt tatgcagtcg ctggacacca ccatcgtaaa
    30841 caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata tgcacatggt
    30901 cattgtctct tatgtgctga ccgtggcggt gatgctgccc gccagcggct ggctggcgga
    30961 caaagtcggc gtgcgcaata ttttctttac cgccatcgtg ctgtttactc tcggttcact
    31021 gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt tacagggcgt
    31081 tggcggcgcg atgatggtgc cggtcggcag attgacggtg atgaaaatcg taccgcgcga
    31141 gcaatatatg gctgcgatga cctttgtcac gttacccggt caggtcggtc cgctgctcgg
    31201 tccggcgctc ggcggtctgc tggtggagta cgcgtcgtgg cactggatct ttttgatcaa
    31261 cattccggtg gggattatcg gtgcgatcgc cacattgctg ttaatgccga actacaccat
    31321 gcagacgtgg cgctttgatc tctccggatt tttattgctg gcggttggca tggcggtatt
    31381 aacccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg attgctggcc
    31441 tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga aataacaacc
    31501 gtgccctgtt cagtctgaaa ctgttccgta ctcgtacctt ttcgctgggc ctggcgggga
    31561 gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt ttcctgcaaa
    31621 ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg gtgcttggca
    31681 gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg ctttggttat cgtcgggtac
    31741 tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt gtttatgact accgccctgc
    31801 tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc aactcgacgc
    31861 gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg gcgagcagcg
    31921 gcaacagcct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc actatcgccg
    31981 ggctgttgct gggacttttt ggttcacagc atgtcagcgt cgacagcggc accacacaaa
    32041 ccgtctttat gtacacctgg cttagcatgg cgttgatcat cgcccttccg gcgttcatct
    32101 ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga aaaaggagcg
    32161 cgcaatgaag ttctggcgac ccggtattac cggcaaactg tttctggcga ttttcgccac
    32221 ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt atcagttttg agcgtggctt
    32281 tattgattac atcaagcatg gtaatgaaca gcgattacaa ctgttaagtg atgcgcttgg
    32341 cgagcagtat gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc gctttgtctt
    32401 tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc cgggtatgcc
    32461 accgcacggc tggcgtaccc agttctgggt ggttgatcaa aacaacaaag tgctggttgg
    32521 tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca acggtgcgga
    32581 agttggcgcg gtgatcgcct cccccgttga gcggttaacg cgcaatactg atatcaattt
    32641 cgataaacaa cagcggcaaa ccagctggtt gattgtcgcc ctggcaacgt tactcgcggc
    32701 acttgccact tttctgctgg cgcgcggttt actggcaccg gtaaaacgac ttgtcgatgg
    32761 cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca gtgaagatga
    32821 actgggcaaa ctggcgcaag acttcaacca gcttgccagc acactggaga aaaaccagca
    32881 aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat tagcggtgct
    32941 gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgtaaa ttcacgccgg agacggtggc
    33001 gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatctcc atcagttgtc
    33061 gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg gtagatttga tcccactgct
    33121 ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagt cgtggcctga aactgcaatt
    33181 ttccctgcca gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc agttattcaa
    33241 taatttactt gaaaacagcc tgcgctatac tgacagcggc ggcagcctga aaatctctgc
    33301 cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag gtgtcagtga
    33361 cgagcagcta caaaaattat ttgaacgttt ttatcgcacc gaaggttccc gcaaccgcgc
    33421 cagcggtggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag cacataatgg
    33481 ccgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag agttaccgct
    33541 ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa cacaccacgt
    33601 attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta tctgcgtgct
    33661 gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccgta tgtgcgccag
    33721 acaccaccgg atctgatcct gttggatctg atgctccctg gcaccgatgg cttgacgctg
    33781 tgccgggaaa ttcgtcgttt ttctgacatt cctatcgtga tggtgacggc aaaaatcgaa
    33841 gagatcgacc gcctgctcgg actggagatt ggcgcagatg actacatctg caagccgtac
    33901 agcccacggg aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa accgcagcgc
    33961 gaattgcagc aacaggatgc cgaaagcccg ttgattatcg acgaaggtcg ttttcaggct
    34021 tcatggcgcg gtaaaatgct tgacctgaca cctgcggaat ttcgtctgtt gaaaacgctc
    34081 tctcatgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct ttatgacgac
    34141 taccgcgtag taaccgaccg caccattgac agccacatta aaaacctgcg ccgcaagctg
    34201 gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgttgg ttaccgctgg
    34261 gaagccgacg cctgccgcat cgtttagttt tagcgacatg actttgttag ccggagatgg
    34321 cgttcagacc agatccggca acattatccc acgcatggtc agcaaactga cattatctct
    34381 ccgccctggc aggcatcaga aaatcgctca tactttaatc ggtaaacagc acctttagat
    34441 gctgttttcg atacgcaatt tcaatcaagg agtcattatg gctggttggt ttgaactcag
    34501 taagagcagt gataatcagt tccggtttgt gctaaaagcg ggcaatggtg agactatcct
    34561 caccagcgag ctgtatacat caaaagcctc tgcggaaaag ggcatcgcgt cagtgcgtag
    34621 caacagcccg caagaagaac gctatgagaa aaaaacggca agtaacggca aattctattt
    34681 caatctgaaa gccgccaatc atcaaattat cggctccagc cagatgtacg ccaccgcgca
    34741 atctcgtgaa accggaattg cctccgttaa agccaatggc acaagccaga cggtgaaaga
    34801 caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc cggctttatt ttctctcatc
    34861 acagagttac tatccctcaa gaaattatta accatgaaat gagttttctc ctcataactg
    34921 ataaggaaaa ttatgctcaa aggattgcgt acattatttt cacgcagggt tttgtttaac
    34981 gtcgaaatga ataattctgt tgactgtgaa ttacttctca tccagcaaca actccaggca
    35041 ttgatagccg aaccaggcaa ttatttagca catgattgtg atctcaacga tactctggtt
    35101 ctttgccagc ttacccatca tcacaatgga tatatacgag aaaaggccgt gcagtatctg
    35161 gggcacaaag aggatgttgc ggctatagaa gaattattac ttgcagctaa tgactgggta
    35221 aaagaggttc gccttgctgc aaccatagca ttacgtcatt taatgaagga taagaatgca
    35281 gcggcatttg ccgttaatct acccatgata cgtaaccttc tccaatgcca gcgttacgat
    35341 caccgtgcat ttgtagacga gatacttaga tatttattga cagaaaataa ccgcccacaa
    35401 ttaacgtatt ggcttacttg ccctgataaa aagctctccc gcgcggtatt acaaacgctg
    35461 attgaatacg gttactttaa tgatgaatct tcactgcttt caatactcga taaacccgat
    35521 gagggtttgc gaatgctggc gaccacccac tggctgcgtc aacaattacc attaagcgaa
    35581 gcggtattaa ctcgtctgtt aaaagaccgc tggccgcgta ttcgacaagc aacgttattt
    35641 agcctcactg accgagcaat agaaatgcct ccggcgttgc acagcacatt actgctggat
    35701 aacaatatgt tgatccgcct acgcgctaaa aatatgcttc acgaagtcat ggatgttccg
    35761 caattctggc gtcatgttgt cacttctgcg gaatatactc cctcacagcg acgggccgct
    35821 ttatacgggc tggattccat tcatgatccc aatattttaa aactggcaga atggggatta
    35881 tcacagaatg tattcccact gcgtt
//
LOCUS       DAJTOR010000020        35515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTOR010000020 DAJTOR010000000
VERSION     DAJTOR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.020"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            108..455
                     /gene="grxD"
                     /locus_tag="OO249_000887"
     CDS             108..455
                     /gene="grxD"
                     /locus_tag="OO249_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5635272.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(506..4913)
                     /locus_tag="OO249_000888"
                     /pseudo
     CDS             complement(506..4913)
                     /locus_tag="OO249_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(5006..5653)
                     /gene="rnt"
                     /locus_tag="OO249_000889"
     CDS             complement(5006..5653)
                     /gene="rnt"
                     /locus_tag="OO249_000889"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5635273.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRSAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMIAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5756..6163)
                     /gene="gloA"
                     /locus_tag="OO249_000890"
     CDS             complement(5756..6163)
                     /gene="gloA"
                     /locus_tag="OO249_000890"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5635274.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6244..7341)
                     /gene="nemA"
                     /locus_tag="OO249_000891"
                     /pseudo
     CDS             complement(6244..7341)
                     /gene="nemA"
                     /locus_tag="OO249_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            complement(7378..7977)
                     /gene="nemR"
                     /locus_tag="OO249_000892"
     CDS             complement(7378..7977)
                     /gene="nemR"
                     /locus_tag="OO249_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5635275.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYTAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8080..8319
                     /locus_tag="OO249_000893"
     CDS             8080..8319
                     /locus_tag="OO249_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5635276.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8368..9264
                     /locus_tag="OO249_000894"
     CDS             8368..9264
                     /locus_tag="OO249_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5635277.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9345..9866
                     /gene="sodC"
                     /locus_tag="OO249_000895"
     CDS             9345..9866
                     /gene="sodC"
                     /locus_tag="OO249_000895"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5635278.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9867..11878)
                     /locus_tag="OO249_000896"
                     /pseudo
     CDS             complement(9867..11878)
                     /locus_tag="OO249_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994342.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11878..12735)
                     /locus_tag="OO249_000897"
     CDS             complement(11878..12735)
                     /locus_tag="OO249_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5635279.1"
                     /translation="MSIKTIKYFSIIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12801..12974)
                     /locus_tag="OO249_000898"
     CDS             complement(12801..12974)
                     /locus_tag="OO249_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5635280.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPL"
     gene            13175..13609
                     /gene="slyA"
                     /locus_tag="OO249_000899"
     CDS             13175..13609
                     /gene="slyA"
                     /locus_tag="OO249_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5635281.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13656..14123)
                     /gene="slyB"
                     /locus_tag="OO249_000900"
     CDS             complement(13656..14123)
                     /gene="slyB"
                     /locus_tag="OO249_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5635282.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14397..15506
                     /gene="anmK"
                     /locus_tag="OO249_000901"
     CDS             14397..15506
                     /gene="anmK"
                     /locus_tag="OO249_000901"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5635283.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VIRPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            15604..15933
                     /gene="mliC"
                     /locus_tag="OO249_000902"
     CDS             15604..15933
                     /gene="mliC"
                     /locus_tag="OO249_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5635284.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15992..16648
                     /gene="pdxH"
                     /locus_tag="OO249_000903"
     CDS             15992..16648
                     /gene="pdxH"
                     /locus_tag="OO249_000903"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5635285.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16777..18051
                     /gene="tyrS"
                     /locus_tag="OO249_000904"
     CDS             16777..18051
                     /gene="tyrS"
                     /locus_tag="OO249_000904"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5635286.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18110..18973
                     /gene="pdxY"
                     /locus_tag="OO249_000905"
     CDS             18110..18973
                     /gene="pdxY"
                     /locus_tag="OO249_000905"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5635287.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(19017..19622)
                     /gene="gstA"
                     /locus_tag="OO249_000906"
     CDS             complement(19017..19622)
                     /gene="gstA"
                     /locus_tag="OO249_000906"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5635288.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNETLKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19728..21230)
                     /gene="dtpA"
                     /locus_tag="OO249_000907"
     CDS             complement(19728..21230)
                     /gene="dtpA"
                     /locus_tag="OO249_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5635289.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21841..22476)
                     /gene="nth"
                     /locus_tag="OO249_000908"
     CDS             complement(21841..22476)
                     /gene="nth"
                     /locus_tag="OO249_000908"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5635290.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22476..23171)
                     /gene="rsxE"
                     /locus_tag="OO249_000909"
     CDS             complement(22476..23171)
                     /gene="rsxE"
                     /locus_tag="OO249_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5635291.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(23175..23795)
                     /gene="rsxG"
                     /locus_tag="OO249_000910"
     CDS             complement(23175..23795)
                     /gene="rsxG"
                     /locus_tag="OO249_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5635292.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23799..24857)
                     /gene="rsxD"
                     /locus_tag="OO249_000911"
     CDS             complement(23799..24857)
                     /gene="rsxD"
                     /locus_tag="OO249_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5635293.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24858..27080)
                     /gene="rsxC"
                     /locus_tag="OO249_000912"
     CDS             complement(24858..27080)
                     /gene="rsxC"
                     /locus_tag="OO249_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5635294.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAIE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(27073..27651)
                     /gene="rsxB"
                     /locus_tag="OO249_000913"
     CDS             complement(27073..27651)
                     /gene="rsxB"
                     /locus_tag="OO249_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5635295.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(27651..28232)
                     /gene="rsxA"
                     /locus_tag="OO249_000914"
     CDS             complement(27651..28232)
                     /gene="rsxA"
                     /locus_tag="OO249_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5635296.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFTAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(28309..28749)
                     /locus_tag="OO249_000915"
     CDS             complement(28309..28749)
                     /locus_tag="OO249_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5635297.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            complement(28835..29050)
                     /gene="ydgT"
                     /locus_tag="OO249_000916"
     CDS             complement(28835..29050)
                     /gene="ydgT"
                     /locus_tag="OO249_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5635298.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(29323..29448)
                     /gene="blr"
                     /locus_tag="OO249_000917"
     CDS             complement(29323..29448)
                     /gene="blr"
                     /locus_tag="OO249_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5635299.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            29661..30701
                     /locus_tag="OO249_000918"
     CDS             29661..30701
                     /locus_tag="OO249_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5635300.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(30736..31737)
                     /gene="add"
                     /locus_tag="OO249_000919"
     CDS             complement(30736..31737)
                     /gene="add"
                     /locus_tag="OO249_000919"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5635301.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(31840..33012)
                     /gene="malY"
                     /locus_tag="OO249_000920"
     CDS             complement(31840..33012)
                     /gene="malY"
                     /locus_tag="OO249_000920"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5635302.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(33022..34614)
                     /gene="malX"
                     /locus_tag="OO249_000921"
     CDS             complement(33022..34614)
                     /gene="malX"
                     /locus_tag="OO249_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5635303.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQTQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            34789..>35515
                     /gene="malI"
                     /locus_tag="OO249_000922"
     CDS             34789..>35515
                     /gene="malI"
                     /locus_tag="OO249_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5635304.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNP"
BASE COUNT         8897 a         9133 c         9247 g         8238 t
ORIGIN      
        1 aaagggataa aatagcaaaa gcgtgatgaa aatgttattt tccgatgccg cactgacccg
       61 ctacaatgtc agattactgc cgtaaagaag tttgaggaag caagacaatg agcaccacta
      121 tcgaaaaaat ccaacgccag attgctgaaa acccgatcct gctgtacatg aaaggttcac
      181 cgaaactgcc gagctgcggt ttctctgccc aggcagtcca ggcgcttgcc gcatgtggcg
      241 aacgttttgc ctatgttgat attctgcaga atccggacat tcgtgcggaa ctgccgaaat
      301 atgctaactg gccgaccttc ccgcaactgt gggttgacgg cgagctggtc ggcggttgtg
      361 atatcgtgat cgaaatgtat cagcgtggcg aactgcagca gctgatcaaa gaaactgccg
      421 ctaaatacaa gtctgaagag ccggacgcgg aataatccgt cctgcaatac tgacggatgc
      481 gaacgggcat ccgtcagtcc tttctctatc cccaatccag cccttgtggc gaacttgaaa
      541 atcccgcctc acgcagcagc gtaaacatcg gcgtttgccg gaccggtaga tcattcactt
      601 ctgttagcgt aaagcgcagc cgtggttcgc gacgcagtgc ggtagtcagc gcgtggaaaa
      661 cctctggggc ttttcctgcc agaccagcat ttttttgcca ccttgcgcca gatagagtaa
      721 caatttgccg ctagaaacga ccaccagcgc acccgcccga cgcgttggaa ccagcgttgc
      781 cggatgtgca ggccagggca gaagatttcc ccacacatta gccggatcgt tggcagagag
      841 cgccactggt gtatagtggc gcgtttgcgt ggcttgtgtc gccagatcgc gccatcggtc
      901 aatagtcaga cgttcagcga attgcgcgcc acccagacct tctacaaaac gacctcgcat
      961 aattcgcccg gagtcttcca tacttcgaca aagcgtttgc atcgatggaa acccgccagg
     1021 gatattttcg gctatcaccg cctgacgact gatgatgccg tagcggtcga gcatattttc
     1081 cgccagcgcc agcatccttt cggtatcgtt tagtggctcc acctgcaata acgaccagcg
     1141 tccagccaga tttggtgtgt tgtaagatac cagcggcgag acgggttgcg catagacagg
     1201 acgtccacga cgagcccggt gactgcggcg agttgaggtg cgtgcgttgg aactgctgcg
     1261 ggtgagggcg cgtaacggtg cccaaatgtc gctggtgatg acgccttgcc agaccagcgc
     1321 ccataacgcc tcttgcaggg cagagagatc aaccgattcg ccgattttgt cgcgtatccg
     1381 ttggctgatt tgttgtgcaa accaggctcc tccgtcagcc agaacagcga ctatcgcttg
     1441 ttgcagcgcc gaacgattcg cctgatccgc ttcggcagga gtgaacgatt ctgcagcata
     1501 ttcctgtaga tgcagtgcca ccaggccgtc atcttcaccc agtttttttt gccccgacca
     1561 gataaccgca ccggttgcca gtaattcatc gagcatttct gacgaatagt cgcgtacgcg
     1621 ggcaggcagg atctggcttt cccagagtga ggcgggtaaa ccgactccgg caagctgttc
     1681 gatcacccgc atcacgccat ctacgccctc ataaacgcct ggcgatgttg aggcaaagag
     1741 cgccgggcta ccatcggtgg cgggtaatac gccctgacgt tccagcagca atcgcgcata
     1801 ggtcgtggct gcaacgggac gcgtcgcttc tctggcggct tgcagcgagc gcaaacgcag
     1861 acgacgaaat acttcatcgc tcacccagat gtcttgttgc agattcatca ccagaccctg
     1921 ttcacgcagt tgctgaagct gctcttcgac aatggcaata ccgagactaa actcatgagc
     1981 cagttgttca gccgtgacca gagcatgagc ccggagatag cgcagaaaga ggtcgcgaag
     2041 cgggtaactg actctatgta aataaatctc tggcaatgac tctggtagtc gtacgccgag
     2101 ggcatcacgc agcctggcgg catcatccat acaggcaaga cgctcctgtc cgctaatcat
     2161 cgctgggaag attcgtttta ctgcaagaag attttccaga tacgacgcaa cctcttcact
     2221 gctgcctgta tgccgttgcg ccaggtcttc aacggtcatt ggccccagtt cgcgcagcag
     2281 gtcgaacaat ccttcttcac ctttcgctct tctgccagga gccagtcgtt gcaactcttc
     2341 ttccacctgg cgaatgacct gcgggtcgag taattccccc ggatcgacct gtccgagtag
     2401 attgcgcagt aactcgctgt ccagcgacag tacggatgcc cggcgctctg ccagcggggc
     2461 gtcgctctgg tacataaatt ccgcgacata gccgaacaat aaacttgtgg cgaaaggcga
     2521 gggcgtagtg gtcgttacat cggatatttg aatttcgcca ccgttcaggc gacgcatcaa
     2581 acgttccagt gcgggaagat cataaacatc ttgcagacat tcgcgtagga tttcgagaat
     2641 gaccggaaaa tctggatatc cctgagcgat ttccagcaac tgactggcgc gcagccgttg
     2701 ttgccaaagc ggggtgcgat ggtccggagt gcgccccggc attaataatg cccgcgcggc
     2761 gcattcgcga aaacgggcgg cgaaaagtgc cgagctgcct accgcctcgc ggacaatttg
     2821 cagcaacttt tctggttcaa acaaaaaaat cgcggcgtcg ggcaatttac catcagtgtc
     2881 aggaatgcgc gcaacaatgc cgtcatcact ggcgaccaca gacgcgtcag cgcccagggt
     2941 tcatgcaccc gtcttccata gggagagtgc aaaataatac gccagtcgcc aatttcatca
     3001 cggcaccgtt cgaggaccag atggcgactc cccggaacga tgcccgtcgc gttgcgttgc
     3061 tcgtcaatca acccctgaat attggcgttc gtattttctt ctgccagcca cgggggaata
     3121 gtcccggaaa agaacgcgcc atccgccagt aaatgaagaa aatcgccgat catctcgcct
     3181 aattcagccg gacgtccgtt accttcacca cgccagaagg ggagccgggc agaacgaccc
     3241 ggagcaggag tcacaatcac ctgatcgcgg gtgatttgct ggatccgcca tgaggtagcg
     3301 ccgagagtga taatgtcgtt cacccgcgac tcatatacca tctcctcatc cagtttaccc
     3361 acccgccgcg aaccggcctt ttcttcacct tcgggtaata acacgctata catgccacga
     3421 tccggaatgg tgccgccgct ggtaaccgcc aacaattgag cgccaggtcg gacggtcaat
     3481 atcccggtct ccctgttcca gaccagtttg gggcgaaaag cagaaaaatc gccagaggga
     3541 tagcgcccgg aaagcatatc cagcgtggcg tcaaaaacac gtcttggcag atctttccac
     3601 ggtgcggcac ggcgtacgcg ggagtaccat tcgtctacct gtaatgcctc catcgccgcg
     3661 gcggcaacgg tttgctgcgc aaggacgtcg agtggattat gcggcggtgt caggttttcc
     3721 agcctgcctg cgaacataca ctctacaatg actgcggaat cgactaagtc acgccgggta
     3781 cgagggaaaa acagcccttt agatacaccg ccaacctgat gtccggcgcg accaatgcgt
     3841 tgtaacccac tggcaacaga aagcggcgtt gccacctgaa tcaccagatc cactgcgccc
     3901 atatcaatcc ccagttcgag actggaggtt gcgaccacgc agcgtaactc acccgatttc
     3961 agcgcctgct cggtgattgc tcgttgttct ttagagacgg agccgtggtg tgagcgggca
     4021 ataaaaacgt cgctactttg tacacggtta gaggttgcgc cggaggtcga ctcgaaatgg
     4081 gccgcatcaa cggcgataga cggggaacgc tgtaagcgtg cggcgtaaag ctcatttaat
     4141 cgtgccgtca gtttttccgc cagcccacgt gaattagtaa agacaatggt cgagcgatgg
     4201 cgcaacactt catcaaggat acccgtttca atatatggcc agatggagcc ttcccggccg
     4261 gcatggctgt cttcgccggt gctgctggcg accgatgaga catcatccat attggcgacc
     4321 ggtacgacaa ttcgtatctg cggatggcgc attgcgggcg ggttgactac cgtaaccggg
     4381 cgatcgccac caagaaaggc tgccacatcg ctggctgagc gcacagtggc agaaaggcca
     4441 attcgctgtg ctgaggtgtg gagcagcgca tcgagccgct ccagacttaa cgccagatgc
     4501 gcaccacgtt tactgcccgc taccgcgtgg acttcatcaa taattaccgt ttcgccgcgc
     4561 cgccgccgtt catcggcaat acccttcaac gggagctgca agttgcgctg aacgtcggtg
     4621 cccagagctt ttatcggtga aatatagagg atgcgtgagg ttttacgctt atgcgcctcg
     4681 cgggtatctt cgccgccttc gcggaagagc cgatcgaggg cgtagagaaa tgctgccagc
     4741 gttttcccgg agccggtcgg tgcaatcacc agcgcatgtt cgcttcgcgc cgccacatgc
     4801 caggtttgcg actggacagc ggtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc
     4861 gccggtgaaa acacgtccgg caggagcgat gaagggtctg gattatctgc cattaacgct
     4921 attccccgtg aatatcatgc aggatgcgtg gaatagcctc actatagatg cctgaatcat
     4981 tgcatgtcat caggcatcga ctcgattaca cctcttcggc ggcaggtagc ggccagcctc
     5041 ccagacgttt ccagcggttg acgatttcac aaaacagcac agcagtgcgt tcggtgtcgt
     5101 acagcgcgga gtgcgcctgg gtgctgtcga agtccatgcc agcggtctgg caagcctttg
     5161 acaatacggt ttgtccgagt gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag
     5221 ggtggaacgg gttacgtttc agtgaggcgc gttctgcggc ggctatcata aagctgtgat
     5281 caaaattggc attgtgcgcc accataatgg cgcggttaca gccgctcgct ttaatacctt
     5341 tacgtacaac tttaaaaatt tcgtgcagcg cctcgtattc gctgaccgcg ctacgatcgg
     5401 gatcgttcgg gtcaatgccg ttgaaggcga gggcttctgg ttgcaaattt gcgccgacaa
     5461 atggttcgac gtggaaatgt aatgtggtgt ccggcatcag ccagccttgt tcatccattt
     5521 tcagggtgat ggcggcaatc tcaagcagcg catcggtttt ggcgttaaat ccggctgttt
     5581 caacatcgat cacaacagga taaaaaccac gaaaacggtc gcacagaccg gtaagttgag
     5641 cgttatcgga catctgattc tcttaatacg gataaattgc agcgcgcatt atgacaaata
     5701 ttacttgtcg atgcagtaaa gatgcgggcg cgatgagttc acgcccggca ggagattagt
     5761 tgcccagacc gcgaccggcg tctttctctt cgattaactc aattttgtaa ccgtccggat
     5821 cttccataaa cgcgataacc gtagtaccgc cttttaccgg acccgcttca cgggtcacgt
     5881 tacccccgtt ttgacggatt ttttcgcacg cttcagcggc gttatctacg ctaagcgcga
     5941 tgtgaccata agcagtgccg agttcgtatt tatccacgcc ccagttgtag gtcagttcaa
     6001 tcaccgcttc ttcggtttcc gggccgtagc caacaaacgc cagtgagtat ttgtattccg
     6061 ggttttcgct ggtacgcagc agtttcatgc ccagcacttt ggtataaaaa tcgatggagc
     6121 gttgcaaatc gccaacgcgc agcatggtat gaagaagacg catcttttta tcctcaaaat
     6181 ggctaacaga ttcaaaatgt tgttttagta tagcggcggc tttacgccgc tcgcaatgtt
     6241 ggattacaac gtcgggtaat cggtatagcc ttccgcgccg ccaccgtaga aactttcggc
     6301 acgctgtggg ttaagctcag ctttgcgctg caagcgggcg accagatccg ggttcgcaat
     6361 ccagtcacga ccaaatgcca ccgcatcaat taaccctttg ccgatcagcg tttcagcttt
     6421 ttctactgtg tatgcacctg cgccgataat cggaccgtgg aaacgggcgc gtactttttc
     6481 gcggaacgca tcagtatacg gttcaccccc cgcccaatct ggttctgaca tatgcagata
     6541 agcaatgccg cgtttaccca gttgttcaat cagatacagt gcatcggctt cttcattcgg
     6601 gccgttatcc gtgttctgga aagtaccgat tggcgaaatg cgaatgccaa tgcgatcggc
     6661 accccattct tcaatcccgg catcgaccac ttccagtacc agacgtgcgc gattttccac
     6721 gctgccgccg tactgatcgg tacgatggtt tgaagaagga gaaaggaact gatgcagcaa
     6781 ataaccgtga gcagagagga gctctaccag atcaaaaccg gcttcacgcg cgttagcaat
     6841 ggcctgacgg aaatcattga cgatacctgg aatctcttct agttcaagcg cacgcggcat
     6901 ggatgtttca acacggatcg cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc
     6961 gctaagtgct gaaggcgcta ccggtgcctg accgccaggt tgcaggctgg cgtgagaaat
     7021 gcgtccggtg tgccacagct acacggccat atgaccattt tcagcatgaa cgccagcggt
     7081 gatttttttc catgcggcaa tttgctccgg actatggatg ccaggcgcac ctgcatatcc
     7141 ttttacctgg gcagaaattt gcgtggcttc actaataatc aaaccggcac tggcacgttg
     7201 gcgatagtat tccgccatca acggggtagg aatgtcaccc ggttcaatac tgcgcagacg
     7261 cgtcagcggt gccataaaaa tacggtttgc cgccgtgatc gcgcccactt tcagtgggga
     7321 atacagtttt tcagatgaca taaaggctcc cggtcgtctg gtgataaaaa atgcctgcta
     7381 aacggcaggc gtcgcaataa tgttttttac atgggccagc gcgttttcca gtggctcgaa
     7441 actgcgcgaa attttggcct gcagattcgc acccagccat agtgcgtaaa gcacttgtgc
     7501 ctgttgtagc ggttcgccac aaaaggttaa acaatggttc tcacggccat tttccagcgc
     7561 ctgagagagc agggcgatca cgccgcgagc acctttatcc atcgcgctgc gcatatcttc
     7621 tgacagatcg cacacttcgg cagagagttt tactgtcagg caaccactga tggttccatg
     7681 ttggcaaaac tggttcagtg tttgctggta ataagccagt atgcggtcgc ggtagttacc
     7741 ttcgccggat tgcagcaact cagtcagtcg ctggtgatat gcggtgtaat gacgctcaag
     7801 catggcaacg ccaaacgctt ctttagagcg aaagtagtga tagaaggacc ctttcggcac
     7861 ttcagcggtt tttagtaatt cgcttagccc catcccggtg aatccacgtt gcaggcaaag
     7921 ttgctcgccc gtcgccagga gatgttcgcg agtatcatgt tcggtgtgtt tgttcatgcg
     7981 ttggagtgta gtagaccagt cggtctagtg caagcaaagt tgctactttg gacattaaca
     8041 gcttcaataa tggtaataat cttatctgag gagggcgaag tggcggagca attagagttc
     8101 tttcccgtcc agagcccgtg ccggggaatt tgccagtctg acgaacgcgg gttttgtcgc
     8161 ggctgttttc gtagtcggga tgagcgtttt aactggaata aaatgagcga tggcgaaaaa
     8221 caagaggtgc tgcgcctttg tcggcagcga ttaatgcgca aattacgcgc aaataagcca
     8281 gcgtcatcag acgaaccgga gcaaccatca ctcttttgaa cctaatcttg cgtatactcc
     8341 tctcatatct gataagagga agcgattatg gttcagcgta ttactattgc gccgcaaggc
     8401 ccggagtttt cccgttttgt gatgggctac tggcgattga tggactggaa tatgtccgcc
     8461 cgccagctgg tcagttttat tgaagagcat ctggatctcg gcgtgaccac cgtggaccat
     8521 gctgatattt atggtggcta tcagtgcgaa gcggcgtttg gcgaggcact gaaactggca
     8581 cctcacctgc gtgaacggat ggaaatcgtc agtaaatgcg gtatcgcgac gaccgcgcgt
     8641 gaagaaaacg tcattggtca ttacatcact gaccgcgatc acatcattaa gagcgccgaa
     8701 cagtcgctaa ttaatctcgc gaccgatcat ctggatctgc tgttaatcca ccgaccagac
     8761 ccgttaatgg atgccgatga agtggcggac gcgttcaaac atctgcatca gagcggcaaa
     8821 gtgcgtcatt ttggcgtatc gaactttacg cctgcgcaat ttgccctgtt gcaatcacgt
     8881 ctgccgttta cccttgccac taatcaggtg gaaatatccc cggtgcatca gccgttactg
     8941 ctggatggca cgctcgacca actacaacaa ctgcgtgttc gtccgatggc gtggtcctgc
     9001 cttggtggtg gtcgtctgtt taatgatgat tatttccagc cgctgcgtga tgaactggct
     9061 gtggtggcag aggagttaaa cgcgggctcg attgaacagg tggtttacgc ctgggtatta
     9121 cgtttaccat cgcagccgct gccaattatc ggttcaggta aaattgagcg cgtacgggca
     9181 gctgtcgaag cagaaacact gaaaatgacc cgtcaacaat ggtttcgtat tcgtaaagcg
     9241 gcactggggt acgacgtacc gtaagcactt ttaggaatag ccgccgttca aaaatgtgtc
     9301 actggtttac acttattcag gaatgcacaa tgaacggagg tcctatgaaa cgttttagtc
     9361 tggctattct ggcgctggtt gttgcaaccg gcgcacaagc tgccagtgaa aaagtcgaga
     9421 tgaacctcgt cacgtcgcaa ggggtagggc agtcaattgg tagcgtcacc attactgaaa
     9481 ccgataaagg tctggagttt tcgcccgatc tgaaagcatt accccccggt gaacatggct
     9541 tccatattca tgccaaagga agctgccagc cagccaccaa agatggcaaa gccagcgccg
     9601 cggaatccgc aggcgggcat cttgatccac aaaataccgg taaacatgaa gggccagaag
     9661 gtgccgggca tttaggcgat ctgcctgcac tggtcgtcaa taatgacggc aaagctaccg
     9721 atgccgtcat cacgcctcgt ctgaaatcac tggatgaaat caaagacaaa gcgctgatgg
     9781 tccacgttgg cggcgataat atgtccgatc aacctaaacc gctgggcggt ggcggtgaac
     9841 gctatgcctg tggtgtaatt aagtaattaa gaggcctgcg taccttgcgg tggtgcttgc
     9901 tcaagttgcg aaagcgagca gtacagccgc cagacaattg ccgccagctc acgggcggca
     9961 ggttgatgat gacgggcaag gctgtcgcaa atccgctgta attcttcaag tgtggccgcc
    10021 agtgattttt gctgaacgcc acgctcactc atcactcccc gcaacagtga aatacagtta
    10081 tcccgtactc gcgataacgg atcggaacgc gattcccagt cgcgcaattg ccagacaaca
    10141 tgagaacagt tcagcagcac tacaccccag cgtaataacc aacggcgagc cagcgcatcc
    10201 tggctgttac tcaactgact gacgtgatga taagtgagcg attcaaattc gctttcactc
    10261 agtgttggat ggcggcttag ctgatcgaca aaatcccggc gcagcgcgcg aatatggcgg
    10321 cggcttttac gagcatccga tcctggacgc agaatggcga acgctagcca agccagcgca
    10381 acgccaacga tttttgccag attatcgtta agaaagtcag caaaatcata caccggggga
    10441 ttagtgacag cgataaacga acccataaaa acaatcagtt gcccccacag tgcggcaaat
    10501 ttgggcatct gcaatttaag aagctgcatt gtcgccagca gtggaaagag aaacagtaaa
    10561 aattgccaca gatcgctaat ctggaccatc aggccaaatt tgaccacaaa gctgaatagc
    10621 gaaagtaaca ccagcgtgcg catcagcagt gacaacgact taaacggtgc tgcgacggcg
    10681 gagtagagta cgcagctaat tgctgccagc gttaatgcat tggcaccggc atcctattgc
    10741 gatgcaatac tccatgcgcc aatcatcatc aacgtacaaa atgtacgcag cccgctccac
    10801 atagcttcgg cgttgtcggt atgacgagcc agagcggatg tccgtgcgag tctggcatga
    10861 tcatctacac cgctttgcag acgatgtaat tcctgactac tttgcagata aaggcggcaa
    10921 aaataacgta gtcgctgcca gaaggcgacg tgccggtagt cggcgacgtt ggtcgggcgt
    10981 agcggggcga taatacgtgc gacggtgtaa gcatctgttt gcgaactggc gagcgccgtc
    11041 agcaattgtt cgagaatttc tcgcgtggca cctggcggtg agggccagtt gagcaacata
    11101 cgtcgcaggc tggagatgac actggtcata cgtaattgct ggtggagtag cgcattaagg
    11161 cgcgcgtttt gctggcgaaa acgatagtgg ctccagaaag cctggatacg cagcaaattc
    11221 atggtcagta tctgcccaat ccccccctca tgtgctgcac gaatggcatc gtttgtttca
    11281 ggctgccaga gtaaactggc atgttccagt aatcgggcgt gcatgttttt caatgcggtt
    11341 aaaagggctg tagcatcgga actgctaggc aggatcatca tcatcatgcc gccgcacaaa
    11401 ataccgacta tcacctcgca aacgcgcgcc tgagcgatat cctacagctg gctggcctca
    11461 gtaatattaa ccatcggaaa ggcgatgatg gcagccgtgt agccgccagt tgaaatgcat
    11521 acgcgacgtt attcgtgaag tgcgcacagg cccaggtaca aaagccaagc cacgccgaca
    11581 tgctcaatag aaaaaaccac ggctcattga gcgtatgccc ggcaagaagc agtgccgcaa
    11641 tggctcccag taaactgcca gcgatgcgtc cgaggctttt gctgatgaca ccgccaacgg
    11701 tgggaaagct aaccactgca gccgaggtca tcgcccaata gggttcatcc agattcaaat
    11761 aataggcaac cgtcagcgcc agacacatgg caatggtatt gcgtaacgca taacgccatt
    11821 gcgccagcgt ggccttgaac cagggcaaat tgcgcaagga ccatgacgat gcgttcatta
    11881 ttgacctacg gcaatgctac aggtggtgcc cgacaccagc gtgacatcgc ctggaacttt
    11941 atccagcgca aagcggacgg gaacgcgttg ggcgagacga acccagggga cgttaggttt
    12001 cacatccgga ataaggcttg agtcactttc aacgctttga tcataaatcg cccgaccgat
    12061 actggaaacg tgaccccgta acgttttgtt atcactatag agggtaattt gtgctggtgc
    12121 accttcgcgg atatggcgca acttggtttc ttcaaaataa ccaatgacgt aaaacgagtg
    12181 gctgtcgaca agggcaaaca gcggtttccc ggtgtcagcg taatcgccga tgcgggtagt
    12241 gaggttagtc acccaaccac tcaccggggc gcgaatttcc gtttgcgcca gttgccattg
    12301 tgtctgtttc agcgtggcct gtgcggcatt aacactggcc tgcatcgctt taacattgag
    12361 gttagcggta tcgagttctt cggcagaaat aaaattttgt gagagatgac gacgacgatt
    12421 cgcttcgtta ttggcttttg cgaggtcaga ttgcgctttc gccagctgag cctgtgcgtt
    12481 cagctcggcg atctgaaagg gcgttttatc gatcgtgagc agaagatccc ccgcgttaac
    12541 cagttggttg tcttttatat tcagctcaac aatacgtccc gacacctgtg gggtgataga
    12601 aaccttttct gcgcgtattt tcccgtcgcg tgtccacgga gattgcatgt aataattcca
    12661 cagccaccat ccggcaagaa ccgcaactat cgctacaatg attattgaga aatacttaat
    12721 tgttttaatt gacatagtta ccacgcaatc agtattgcca gagcaaggca aacgcaaatc
    12781 gcaaacagcg ataaatccat ttacaaggga tgccagatgt caccggcgta gatccagcca
    12841 cgaagcaggc ggtgtacgac aagccagatg acgaatccaa acgcgaaggc tttgaaaaac
    12901 ggaggaaaat agacggacgc accgaacacc agatcttgta agggcaatcc tgttgcattg
    12961 agcataaact tcacaaagta gattccttta cgaccgaata tattgcgtta gattaataaa
    13021 tattctttaa gtgcgaaaaa tttacgcgca atttctgaaa aatatgtctg acggtaacca
    13081 aatgcagcaa tacatttgtt ttagcaataa aattgctgca cactattcta aaagccgcat
    13141 aatatcttag caagctaatt ataaggagat gaaattggaa tcgccactag gttctgatct
    13201 ggcacggttg gtgcgcatat ggcgtgctct gatagaccat cgcctgaaac cgctggagtt
    13261 aacacaaacc cattgggtta cgttacacaa tatccatcag ttacctccag accagtcgca
    13321 aattcaactg gcaaaagcga ttggcatcga gcagccatca ctggtccgta ctctggacca
    13381 actggaagaa aaagggttaa tttcgcgtca aacttgtgcc agcgatcgtc gggctaaacg
    13441 tattaaactg acggaaaagg cagagccact gatcagcgaa atggaagctg ttattaacaa
    13501 aacccgcgcg gaaatattac atggcatctc cgcagaggaa ctggagcaac tgattacgct
    13561 catcgcaaaa cttgagcata atatcattga gttacaggcc aaagggtgaa atgaaggggg
    13621 cgtgtggtca gtaacctgac cacacgcaaa cttatttagc gcggagaaac ggtcacctga
    13681 ctgccattgc tggccagtac gacacgttgg cccggagaga aacgagtgtt gccttgtttc
    13741 tgtaccacca tgatggtatt accatcgtct ttacgaattt ccagctcgac accctgcgtt
    13801 ttgttcattg cactctgtac gccctgacca gctacgccac ctgcaacagc gcctgctgca
    13861 gtagccagag aacgcccggt tccgccacca acagtattcc ccaggaaacc accaagaaca
    13921 gcaccgccaa ttgcaccgat aacgttggaa tcatcaccgc cctgaatctg taccggacgt
    13981 acgttaacga tggtgccata gctgacattc tgtacttgtt tcgcttcaga agcggtataa
    14041 acatcccctg acagggtgtc gttattaaca caaccgacaa gagacagacc taccattgaa
    14101 acaaccaata cgcgtttaat cattgaaaaa tctcctgttc accatgaaac gccacgcaag
    14161 catccctcat ggtcaaagta tatggcatat gtgaatccat ggttacatgt tctaccaaat
    14221 atgagcaaat cataatgaaa gtaggatcaa ccaattataa acaaaacatt catgatgatt
    14281 attgatttga agataatgtt ttactaccaa cggtaaaaat cggtcattcc gcatggtatt
    14341 cacgaaaagt ttatgtttga gtggtgcgca aacgtcagat tgaaaaagga tgaactatga
    14401 aatcgggccg ctttattggc gttatgtcag gcaccagcct tgatggtgtt gatgttgtgt
    14461 tggcgacaat agatgaacac cgggtcgcac agctggcaag cttgagctgg ccgatcccgg
    14521 tgtctctgaa acaggctgta ctggatattt gccagggcca gcagcttaca ctttcgcagt
    14581 ttggacagct tgatactcaa ctcgggcgac tttttgctga tgcggtcaat gccttgctta
    14641 aggaacaaaa cctgcaggcg agagatatag ttgcgatcgg ctgtcacggt caaaccgtct
    14701 ggcatgaacc gacgggcgtg gcaccacaca ctttacagat tggcgataac aatcaaattg
    14761 tggcacgcac cggaattacg gttgtcggtg attttcgccg tcgcgatatt gccttgggag
    14821 gacaaggcgc accgctggta cctgcgttcc atcatgcctt gctggctcac ccaaccgagc
    14881 gacgaatggt gctcaatatt ggcggcatcg ccaatctgtc actgctcatt cctgggcagc
    14941 cggttggcgg ctacgatacc ggtcctggta acatgctgat ggatgcctgg atctggcgtc
    15001 aggccggtaa accttacgat aaagatgccg agtgggcacg ggcgggtaaa gttattcgcc
    15061 cactgctgca aaatatgctc agcgacccat atttctcgca acctgcaccg aaaagcaccg
    15121 gacgcgaata ctttaactac ggctggctgg agcgccattt acgccatttt ccgggtgttg
    15181 atccccgtga tgttcaggcc acactggcag aactcaccgc cgtgaccatt tctgaacaag
    15241 ttttgttgag cggtggctgc gaacgattga tggtatgtgg tggaggtagt cgtaatccgc
    15301 tactcatggc gcgtctggcg gcattactgc caggcacaga agtcaccacc accgatgccg
    15361 ttggcattag tggcgatgac atggaagcat tggctttcgc ctggcttgcc tggcggacgc
    15421 tggcgggatt accaggaaat ctgccttccg tcactggcgc aagccaggag acggtactgg
    15481 gggctatttt ccctgctaac ccgtgatatc agagttaact gaatttttct taccgcgtct
    15541 gccgttacac ttggaacgtt agggagggcg tattgccctc cagaccagga aagtcttcgg
    15601 gaaatgtcta tgaaaaaact gttactgatc tgtttgccgg tgcttctcac cggctgtagc
    15661 gcgttcaatc aactggttga gcacatgcaa accgctacgc tggaatacca gtgtgatgaa
    15721 aaaccgttga cggtcaaact gaataatccg cgccaggaag tcagttttgt ttacgataat
    15781 caactactgc atctcaaaca gggcatttca gcgtctggtg cacgttacac tgacggaatc
    15841 tatgttttct ggtcgaaagg cgatgaagcg actgtctata aacgcgaccg catcgtattg
    15901 aataactgtc agttacaaaa tccacagcgt tgagattttt ccaggggcgg cgcacaatag
    15961 cgtcacccac tgacaatccg taaagaaaac catgtctgat aacgacgaat tgcagcaaat
    16021 cgcgcatctg cgccgtgaat acaccaaagg cgggttacgc cgtcgcgatc ttcccgccga
    16081 tccattaacc ctttttgaac gttggctctc tcaggcgtgt gaagccaaac tggcagaccc
    16141 taccgcgatg gtggtcgcta ccgtggatga acatgctcag ccttatcaac gcatcgtttt
    16201 actcaaacat tacgacgaaa aaggcatggt gttttacacc aacctcggta gccgtaaagc
    16261 acatcaaatt gaaaataatc cgcgcgttag cctgctgttc ccgtggcata cccttgaacg
    16321 ccaggtgatg gtgatcggta aagcagaacg actttcgact ctcgaagtga tgaaatattt
    16381 tcatagccgc ccgcgtgata gccagattgg tgcatgggtt tcgaagcagt ccagtcgcat
    16441 ttctgcccgc ggtatccttg aaagtaaatt cctggagctg aagcagaagt ttcaacaggg
    16501 cgaagtgcca ttgccgagct tttggggcgg ttttcgcgtc agccttgaac agattgagtt
    16561 ctggcagggc ggtgagcatc gcctgcatga ccgctttttg taccagcgag aaaatgatgc
    16621 gtggaagatt gatcgtcttg caccctgaaa agatgcaaaa atcttgcttt aatcgctggt
    16681 actcctgatt ctggcacttt attctatgtc tctttcgcat ctggcgaaaa gtcgtgtacc
    16741 ggcaaaggtg cagtcgttat atacatggag attttgatgg caagcagtaa cttgattaaa
    16801 caattgcaag agcgggggct ggtagcccag gtgacggacg aggaagcgtt agcagagcga
    16861 ctggcgcaag gcccgatcgc gctctattgc ggcttcgatc ctaccgctga cagcttgcat
    16921 ttggggcatc ttgttccatt gttatgcctg aaacgcttcc aacaggcggg ccacaagccg
    16981 gttgcgctgg taggcggcgc gacgggtctg attggcgacc cgagcttcaa agctgccgag
    17041 cgtaagctga acaccgaaga aactgttcag gagtgggtgg acaaaatccg taagcaggtt
    17101 gccccgttcc tcgatttcga ctgtggagaa aactctgcta tcgcggcgaa caactatgac
    17161 tggttcggca atatgaatgt gctgaccttc ctgcgcgata ttggcaaaca cttctccgtt
    17221 aaccagatga tcaacaaaga agcggttaag cagcgtctca accgtgaaga tcaggggatt
    17281 tcgttcactg agttttccta caacctgttg cagggttatg acttcgcctg tctgaacaaa
    17341 cagtacggtg tggtgctgca aattggtggt tctgaccagt ggggtaacat cacttctggt
    17401 atcgacctga cccgtcgtct gcatcagaat caggtgtttg gcctgaccgt tccgctgatc
    17461 actaaagcag atggcaccaa atttggtaaa actgaaggcg gcgcagtctg gttggatccg
    17521 aagaaaacca gcccgtacaa attctaccag ttctggatca acactgcgga tgccgacgtt
    17581 taccgcttcc tgaagttctt cacctttatg agcattgaag agatcaacgc cctggaagaa
    17641 gaagataaaa acagcggtaa agcaccgcgc gcccagtatg tactggcgga gcaggtgact
    17701 cgtctggttc acggtgaaga aggtttacag gcggcaaaac gtattaccga atgcctgttc
    17761 agcggttctt tgagtgcgct gagtgaagcg gacttcgaac agctggcgca ggacggcgta
    17821 ccgatggttg agatggaaaa gggcgcagac ctgatgcagg cgctggtcga ttctgaactg
    17881 caaccttccc gtggtcaggc gcgtaaaact atcgcctcca atgccatcac cattaacggt
    17941 gaaaaacagt ccgatcctga atacttcttt aaagaagaag atcgtctgtt tggtcgtttt
    18001 accttactgc gtcgcggcaa aaagaattac tgtctgattt gctggaaata atgcattaag
    18061 tggaaagggg gagtgagaaa tcactccccc tggtttttat acagggaaca tgatgaaaaa
    18121 tattctcgct atccagtctc acgttgttta tggtcatgcg ggtaacagtg cggcagagtt
    18181 tcctatgcgc cgcctgggcg cgaacgtctg gccgctgaac accgttcaat tttctaatca
    18241 cacccagtac ggcaaatgga ctggctgcgt gatgccgccc agccatttaa ccgaaattgt
    18301 gcaaggcatt gccgccattg ataaattaca cacctgtgat gccgtgttaa gtggctatct
    18361 gggatcggcg gagcagggtg aacatatcct cggtatcgtc cgtcaggtga aagccgcgaa
    18421 tccgcaggcg aaatattttt gcgatccggt aatgggtcat ccggaaaaag gctgtatcgt
    18481 tgcaccgggt gtcgcagagt ttcatgtgcg gcacggtttg cctgccagcg atatcattgc
    18541 gccgaatctg gttgagctgg aaatactctg tgagcatgcg gtaaataacg tcgaagaagc
    18601 ggttctggca gcgcgcgaac tcattgcgca agggccacaa attgtgttgg ttaaacacct
    18661 ggcgcgagct ggctacagcc gtgaccgttt tgaaatgctg ctggtcaccg ccgatgaagc
    18721 ctggcatatc agccgtccgc tggtggattt tggtatgcgc cagccggtag gtgttggtga
    18781 tgtgacgagc ggtttactgc tggtgaaact gcttcagggg gcaacgctgc aggaggcgct
    18841 ggaacatgtg accgctgcag tctacgaaat catggtgacc accaaagcaa tgcaggaata
    18901 tgagctgcaa gtggtggctg ctcaggatcg tattgccaaa ccagaacatt acttcagcgc
    18961 aacaaagctc tgaaatatct catttcggcc cacaacgacc tgtgggccga tggctattac
    19021 tttaagcctt ccgctgacag tgcgtcttgt acttccggac gttcagccat acgttgcata
    19081 aatgctgcaa tgtgctctaa cccttccaga ttcagtttca ccgcgtatgc ccagcgcaga
    19141 accgtaaaca gataggcatc agcaattgta aatctttgcc cgcagatcca gtgctcatcc
    19201 ttcagtgtct cgttcacata ttgcagcttc ttctccagct gcgcgcgaac tgtcggtttg
    19261 tactcttccg gtgtatcagg gcgaaacaga ggtgtgaaac ctttatgcag ctcggtggcg
    19321 atgtaattca gccattcgat ggttttatag cgggaaatac tgtttaccgg tgccagcaac
    19381 tggcggtcgg ggacgctgtc ggcaagatac tgcataatcg ctacgccttc cgtcagcaaa
    19441 gtaccgtcat ccagcagcaa tgcaggcacc tgccccttag ggttaacggc aaagtaatcg
    19501 tcaccgtttt cgagacgttt tttcattaaa tccacactga cgagggtaaa atcctttccg
    19561 ctctcacgca gggtgatatg ggaagcgaga gagcaggcac ccggtttgta gaacaatttc
    19621 atcggtaact ccttttgtct gtggtttaaa gtatggtagt gcgttgctga acaaaaaaaa
    19681 agccgctaac gaaaagttag cggcttgtaa aagagtttcc ctaaatttta cgctacagca
    19741 gctttcgccg ctttgtctgc agcgtcatcc tgcgtcatac ggtgcagttt aggcgcggtc
    19801 agcagcatca gtactgcaat aatggcagta gcgacaccaa tctgcaagaa tacgcgacca
    19861 taaacttcca gtgacatcag cgggtcggta acgttatccg gcacagccat catacccgca
    19921 acataaccac caatcaggtt tgcaccggca gtggtcagga accagctacc cataatgaag
    19981 cccatcagac gctgcggaac gagttgtgca accattgcca gacccagacc agagatcatc
    20041 agttccccga tgctctgcag gccatagctt gcgaccagcc agcttacaga cacgatacca
    20101 gcgtcagacg cgaatttcgc acccagcggc agaatcagga acgcaccaga acacatcacc
    20161 atgccgattg caaacttggt tggcatcggc agggtatcgc ccatcttgtt atagatagcg
    20221 gccagaatcg gactaccgat gatgatccag aacgggttca gtgcctgata ctgctcaggt
    20281 tctacggcca gacccagaat ggagtgctca acgttacgaa tcgcaaagaa gttcagtgac
    20341 gttggcatct ggctgtacag cacgaagaag ataatggctt cgagcatcag gatgaaggca
    20401 acgatcattt tacgacgcgc agcacctttc atcgcgaagg cttctttacc gaagataacc
    20461 acgataccga aggcaacaac gcccagcgcc atacgcgcaa cttcctgatt gtgcagcagc
    20521 caggtggcga tagcgatcag tgccacaaca ccaataatgg tcagtagcag gttacggtag
    20581 ttgataggct cgaagtctgg ttttgaaccg tactgtttaa cccagcgttg gcagaaggcg
    20641 aagttaacga tagtgatcag caggcctaca acgctcaacg caaacgcaac actccagccg
    20701 tatttcgcgg ccagccacgg cgtagcaatc atagagaaga aagagccgat gttgacggac
    20761 atgtagtaca tggtgaatgc accgtccaga cgcgggtcgt ttttctcata gcatgtagaa
    20821 agcagagaag acgggttagc tttaaacagg ccgttaccga ccgcaatagc cgccataccc
    20881 atataaacga taccggcgtc gtgaccagac caggcaacca gagcataacc aatcgccagc
    20941 acaatagcgc cgagcataat tacgcgttta gtacccagta ccttgtcacc taaccagccg
    21001 ccgatagcga ccagaccata aaccagggca ctaaaggaag agaaaagggt gattgaatcc
    21061 gcttcagaca tacccagttg tttaaccagg taaacagcca taattccttg taggccgtaa
    21121 taaccaaaac gttcccataa ctcaatcgag aagatgagat agaacgcctt cggttgtttg
    21181 aaagcgttca aactgacgct ttcagttggt ttttggtttg cagtggacac ttttacctct
    21241 ttttttacat cccatattaa cgggggtgct ttctcgcgtt atgtctaaaa aacatacgcg
    21301 caattgttat agtgggaggg gaaacggcag gtaatgttca ctatcttgca ctttcagaca
    21361 agtcctttgt aataatttgt tacatataac tgaggcgggg aattcaacca ttccgggaaa
    21421 tgttatccag cgttaaattt ttcaaaaaac gtaacggcag attttttcac tgcactgaga
    21481 ggggggtgat ggcttaacgg cgatatgttc tgctatttag cgtttttact agtgagatag
    21541 tccagtttct gaaaaatagc cagtgtaatg ttttgtaggt caatatttgt tgcgtgtgta
    21601 aatttaaatg attttaacct gattcttgca atgtttttac attgcgacag catcgtgatc
    21661 ttgatcacga atgaatagaa aatttcgcta ttaaaaaaac aattaacctg catttttggt
    21721 tatccgcaaa cggattacct gatgataacg ctaagcaatg gcatcattca cgttaaaaag
    21781 atgttttaaa agaggataaa gaaaggttat caatggggta atcggtgtta ccccttttct
    21841 tcagatgtca actttctctt tgtattcaca aagatcttca ataatacaag agccacagcg
    21901 gggcttgcgg gcaatgcagg tataacgccc gtgcaggatc aaccaatggt ggcagtcgac
    21961 tttaaactct gccggaacca ctttcagtag cttttcttct acctgttcaa cgtttttccc
    22021 cggcgcaaat tgagtacgat tacaaacgcg gaaaatgtgc gtgtcgacag caatagtcgg
    22081 ccagccgaat gcagtgttta atacgacgtt ggctgtttta cgacctacgc cgggcagggc
    22141 ttcaagcgca gcacgatctt ccggaacctc gccattatgc tgctccagta agatacggca
    22201 ggttttgatg atattttctg ctttgctgtt ataaagccca atcgttttga tataggtttt
    22261 aaccccttca acgcccagtt caagcatcgc tgcaggcgta ttcgccaccg ggtagagttt
    22321 cgccgtcgcc ttattaacac tgacatcggt cgcctgagcg gaaagcagta cggcaatcag
    22381 caattcaaaa ggcgaactga aattaagctc ggtggtggga tgaggattgt tctcacgcag
    22441 gcgagtgagg atctccaggc gttttgcttt gttcatcaga cattccctgt ttcaccgttt
    22501 ggcaatgcac gttcggcagc tgcttcagta cggcgctttt tcattctttc atcaatcagg
    22561 tattttcctg ccagcatcag tcccaggcca ataaatgcac ctggtggcag catcgccagc
    22621 aggaaagggg agtcggtgtg gaaaatctcc acgcgtaata cttttgccca gctacctaac
    22681 agcgcatctg caccgtcaaa caatgtgcca ttgccgataa tttcgcgtag tgaacccagc
    22741 acgaacatgg cgcaggttgc gcccatacca attgaaaagc cgtccagtgc cgaaagcgcc
    22801 ggaccttttt tggcggcgaa ggcttcagcg cggcccacaa cgatacagtt agtgacaatc
    22861 agcggaataa aaatccctaa tgattgatac aggccaaagg cgtaggcgtt gatcagcatc
    22921 tgtacagcgc tgaccaccga ggcgatgatc atcacgtaaa tgggaatgcg gatctcggct
    22981 ggcgtccagt gacgcagcgt cgaaatggtc aggttggtca gcgtcagtac cagcgtagtc
    23041 gcaagtccta aacccagagc attagtggca gtggacgtga ccgccaacag aggacaaagg
    23101 ccgagcaact ggaccagcgc agagttgttt ttccacaacc cctgaacaat aacgtcttta
    23161 atttcgctca cgctttattc tccacaggca ggaagttgag aaagttgtgc cggtaacgtc
    23221 tgagcgtaca atccggcgcg ttttaccgca ttaaccaccg cgcggggagt aatcgtcgcg
    23281 ccggtgaact ggtcgaaatc accaccatct ttcttcaccg cccagtgcgc atcatccgca
    23341 ccactgattt ttttacccgc aaaatgggtg atccagtcag aaaggcgcag ttcgatttta
    23401 tcgccaagcc ctggcgtttc gtggtgctct gtcacgcgcg tgccaagtac cgtgccgtta
    23461 aaatcggctc ccaccagcag ctgaatcgca ccggaatagc catctggcgc ggttgcttcc
    23521 agaacggcgg ctaccggttt gtcatcctgt ttggcgatgt aaacccgatg ctcaccttta
    23581 cctaactctg gcgcagttac cagatagcaa ctctgtgcca gcgcattgtt atagcgttcg
    23641 gctggcagca cctgatcaaa taatgccttt tgttgcagac ttgcctgttc agcaatcgtc
    23701 gttttggtca tctggttgat ggccgcagtt aaccctgttg aacccgctgc aaatagcgct
    23761 aacgtaatgc cgtgttttcg gatagttttc agcatggttt accctttgcg atggccgtag
    23821 acgcgcggac gcgtgtagta atcgatcaga ggaaccgtga tgttcgccag caggacggca
    23881 aaagccacgc cgtcaggata gccgccgaaa ctgcggatca accagactaa taagcccgca
    23941 agcgcgccga aaataagacg accacgattg gtcgtagaag cggtaaccgg gtcagtcaaa
    24001 ataaagaatg cgccgagcat ggtcgcacca gacagcagat gaatttgcgg tgctgccagt
    24061 gtttctggtg agaacaacca gcccaacgtt gcgcataacg ccagcgttac taagaagctg
    24121 aggggaatat gccagcgaat cgctttctgc catagcaacc acaggccgcc agccagccag
    24181 gcgagattta cccattgcca accagcgccc gccagaatac cgctgtagat cggatattgc
    24241 ataatctgtt caaccgaatg accggcacgg acagaggttt taaatgtatc cagcggtgtc
    24301 gcctgactaa tgccatcaat acctaagtgt agtgtgttca tatcagcacc actggcggta
    24361 tgcccgctga aaataacctg tatggcgtcg ataaaaccag ggatgttgac cgcaatttca
    24421 tgtggcggta accagctggt catctgcacc gggaaggaga tcagtaagac cacataacca
    24481 atcattgccg gattaaacgg gttttgcccc agaccgccat acaactgttt agcgatgatc
    24541 accgcaaaca ccgtacccag cacgaccatc caccatggcg cgaggggggg aatacttacc
    24601 gccagcaata agcctgtcag caatgctgag ttatctttca acgttgcggc taccgactgc
    24661 ttgcgtagtt tgagtacgag agcttcggct aacagagtac taaccgatgc caacaggatc
    24721 tgaacgagag taccccaacc aaaaaaccac agttgcgctg cgattcctgg cacggctgcg
    24781 agcaacacca acagcataat gcgcgatgtc tggcgctggt tatgggtata aggggagcta
    24841 gctattctga ataccattta gtcctcgttt acaacctttt gctgggcggc ttttttggcc
    24901 tgagcgcggg caatagccgc ggcaactgcc gctttgcgcg gatcaacctg ttcttctggt
    24961 tctgcgttag tctgttgctg ttccagcttg cgcgctttgg cacgggcaat agcggcttcg
    25021 acggcggctt tgcgcggatc aacctgttct tcaggtaccg cattagcctg ttgctgttcc
    25081 agcttgcgtg ctttggcgcg tgcaatagcc gcttcgacgg cagctttgcg cggatcaacc
    25141 tgttcttctg gctccgcatt agcctgttgc tgttccagtt tgcgcgcttt ggctcgggca
    25201 atagcggctt cgatggcggc tttgcgcgga tcaacctgtt cttctggttc cgcattagcc
    25261 tgttgctgtt ccagcttgcg cgctttggca cgggcaatag cggcttcgac ggcggctttg
    25321 cgcggatcga cctgttcttc tggttccgca ttagcctgtt gctgttccag cttgcgcgct
    25381 ttggcgcggg cgatagctgc ttcaacggca gttttacgtg gatcagcaac ggttgctgcg
    25441 tcgttagttt gctgcagttc tgcctgtttc gctctggctt gcgctttacg ggcttcccgt
    25501 gctgcaataa ttgcactgtt atccgggcgt tcgcccgctt taatcacaat aggctgtgta
    25561 gcctgggcct gtttctcttt cacccgcgcc agagcggcag caatcgcatc tttatctttg
    25621 gctgcaggtt gaacggctgc gctcttatgt cgttcaaggc gagccgcttt ttcgcgctcc
    25681 agacgagcct ggcgcgcttc gaaacgcgct ttggcttctg cggcgcgctt ttcttcctga
    25741 cgaatagccg caatttcagc tttttcctga cggaaatatt gcaccagggg aatattgctc
    25801 gggcaaaccc acgcgcaagc cccacattca atgcaatcag caatgttatg cgtggtagct
    25861 ttatcgtgtt gctgaccttt gctgaaccag tacaactgtt gcggcaaaag atccgcaggg
    25921 caggcgtcag cacaggcgct acaccggatg cagctttgtt cttcctgtgg ttcgccaagt
    25981 tcattggcag agggagccaa cagacagttg gtaatcttta cgaccgggac atccagccat
    26041 ggcaaggtaa agcccattag cgggccaccc ataatcacca tttgatcggc agaggggcag
    26101 aatcgggcat cattcaataa atgacgcact ggcgtcccca gccgtgccca gacgttgccc
    26161 gggcgagcga ttgcttcgcc agtcagggtt acaacacgct cggtaatcgg ctcgccatca
    26221 ataacggcac gtttcactgc ataagcagtg ccgacgtttt gcattaatac gccgatatcg
    26281 gatgaacgcc cgccatgtgg aacctgcttc ccggtcagaa tgtaggttaa ttgtttagca
    26341 ccgccagaag gatatttggt tggaatcacc cgcagagaaa tatcgttaga gtccgccagc
    26401 accgcgcgca gcatggaaat cgcctgcggt ttgttatctt caatgccgat aagaatttcg
    26461 cgtggctgca gaatatgcgc aagaatgcga ataccctcta cgacctgagc cgcgcaatcc
    26521 tgcatcaaac ggtcatcggc ggtaatgtac ggctcgcact cagccgcgtt gataatcaac
    26581 gtttcaatct tatctccgcc accctgcaat ttaacgcctg tcgggaatcc tgcaccgccc
    26641 agcccggcaa caccaaactg atgtatgcgc tcgattaact cttcgcgact acgagtgcga
    26701 taatcggccc agccgtcgcg cgggatccag cagtcttcac catcggcatc aataatcacg
    26761 cttaattcag ctaaagctga aggatgagcc gtagagtggg gcgcaatagc cgtaacggta
    26821 cccgaggtgg gcgcgtgaac aggcagcatt ttgccgcgac cacgggtaag cggctggccg
    26881 cgcaatactt tatcgccgac gctaacgcac aactcacctt cagcgccaat atgctgtttc
    26941 agtggaataa caaaacgctg cgccagtggt acctggcgca ggggtgtacc gttggactgg
    27001 gttttcatct ccggtggatg gatgccgccg ttgaaatccc agattttatt ttttctgaat
    27061 gcagagaata acttaagcat ggtgttccac gggaatgata cgcacgggaa tggtgttcag
    27121 atcccatttc caggagtcag gtgtttctgc gaccggttgc aacgagatgc agtgcgtcgg
    27181 gcacggatca acacataaat tgcagcccgt acagagatca ctcattaccg tatgcatggc
    27241 acgggtagcg ccaacgatgg cgtctaccgg acaagcctga atacatttag tgcagccaat
    27301 acagttattt tcatcaataa ccgccaccat ccgcgcaggc gttagctctt gcgcttcgcc
    27361 atccagcggc tgcggctcga cattaagcaa ctcggcaatt tttagcatca cagcttcgcc
    27421 acctggggcg caacggttga ttttttcacc gttacagctg atggcttccg cgtaggggcg
    27481 acagccggga taaccgcact gaccacactg gctctgcggt aagatttcgt caattttctc
    27541 aacgaccgga tcgtcttcca ccgcaaaacg gcgggaggca taacccagaa tggcgccaaa
    27601 cgccaggccc agcaggctca cggcggcaac ggcaatccag atagcattca ttacaacttc
    27661 accaaaccac taaagcccat aaaggccaga gacataagac ctgcggtaat taacgcaatg
    27721 gcattaccgc gaaaaggtgc cgggacatca gccacagcaa ggcgttcgcg gatggcggtg
    27781 aagagcacca tcaccagcga gaaaccgaca gcggcggaaa aaccgtacag cgccgactgc
    27841 aagaaattgt gcccgagatt gatattcagc aacgccacgc cgagcactgc acagttggtg
    27901 gtgataagcg gcaaaaaaat ccccagcaga cggtaaagca ccgggctggt tttgcgcacc
    27961 accatctcgg tgaactgcac gaccacagca atcaccagaa taaatgccag ggtgcgcagg
    28021 taaatcagat taagtgggat caaaatccac gtatcgataa gccaggcgca aatagacgcc
    28081 agcgtcatca caaacgttgt tgccagcccc atgcccatcg cggtttccag ttttttggaa
    28141 acccccataa acggacagag accgagaaac ttgaccagca caaagttatt gaccagtaca
    28201 gttccgacaa acagtaacag gtagtcagtc atttttcggc ctgaaataaa aaagccgcct
    28261 attatccgtt aatccagagc aggcgacaac aggttaactg taaggttatt acggattcac
    28321 aaacgtcgct tttacccgcg acgagcgttt gaaataggag acgatcaatg cggttgccaa
    28381 caaagtaaac agcaactggc gaacggctat gccatcttcc acgggtgaaa aagcgaatgc
    28441 ttttactgcc agcaatacgg aaataagcag ccagataata tagtgtttag gaacacaacg
    28501 ccgacgttta aagaaagcga tggtcaacca cagtgtgtaa taccacatgg cgatagcggt
    28561 aataaaagaa acgccccata agatttgtgt cgtcagtgct tgtccgccga gcgtttgaaa
    28621 tgtttgcgga gaagataacg cagcggtata cagcaacaac gccagcgtcg tactcaacag
    28681 agcgactaaa agccaggcca acgggccaag caaccagcct ccaatacgtt gtggcgttgt
    28741 ggtggtcata ggttctccag aatagcgacg caaacaggca aatctaattt taggggcgtt
    28801 attataaggc ttttataacc gatcgctact ttctttattg gacatagtgc cagacggact
    28861 tcggtacctg gccgaggtca aacaaacgcc cgccagaaac cagctctgcg cgacggtgat
    28921 cggcagcacg atacatattg atcagttcct gatcgtccgt cagggtgtaa ttaagatggt
    28981 cgtagagttt ttccagactt tcgagtgaac tgattttgcg aaattttaat aagtagtcct
    29041 gaacagtcat aaaaggtcca taaaataatt aataccaaag gggtaggtat tattagtgat
    29101 gataaaattt caccaaaagt gaacgcttgt gttattacaa taataagaat gcgctgatgg
    29161 caaatattca gggatgaaaa actgctctga aaagagtaac gcgctttact atccctgaca
    29221 atcaccaaca acatcgaaca agataataaa ttcctggttt aatatccgac aagtgaaaac
    29281 atgcaaccgg acgggcagca tggcgctcta caagcgcaga gcttacttgt gttgtaccga
    29341 agcactctgt tcaggtggct gatagttgtc aatgtgactc gccacgccaa gaagaatgac
    29401 tgaaacgaca agaacgatcc aacctgttaa ttcaataaga cgattcatta cagcccacat
    29461 ctctcttgat tgatccatta acttcagggg gtaaatgtta cttagcaata atagctccag
    29521 caagattttt actgaggttt ttgcgatatt tagcttttgt cgttggaaaa ttcgctattt
    29581 ttttgactta atattaaact gttgagcgtg ggtaaaggat agtgtcaaat agcaatcata
    29641 cttcaatgag aggcaatgac atgagcgaca acatccgtgt tgggttgatt gggtatggtt
    29701 atgcgagcaa aaccttccat gcgcccctga ttgcgggcac gcccgggctg gaactggcgg
    29761 taatctccag cagtgatgaa acaaaagtaa aagccgactg gccaacggtt acggttgtct
    29821 ctgagccgaa gcatctgttt aacgatccca acatagacct gattgtcatt cctacaccca
    29881 acgataccca tttcccgtta gccaaagcgg cgcttgaggc gggtaaacat gtggtcgttg
    29941 ataaaccctt taccgtgaca ctgtcacaag cgcgagagct ggatgcgctg gcaaaaagcc
    30001 tggggcgtgt gctgtctgta ttccataacc gtcgttggga tagcgatttc ttgacgctaa
    30061 aaggtttgct cgcggaaggc gtgctgggtg aagttgctta ctttgagtct cattttgacc
    30121 gcttccgtcc gcaggtgcgc gatcgttggc gtgaacaggg cggcccaggc agcggtatct
    30181 ggtacgattt agcaccgcat cttcttgatc aggccattac gctatttggt ttaccggtca
    30241 gcatgacggt agatttggca cagttacggc ccggagcgca gtcgaccgat tatttccacg
    30301 ccatcttgtc ctatccacag cggcgagtca ttttacacgg taccatgctg gcagccgctg
    30361 agtcagcacg gtatatcgtg catggatccc gaggcagtta tgtgaaatat ggcctcgatc
    30421 cacaggaaga acgtctgaaa aatggcgagc gtctgccgca ggaagactgg ggctacgata
    30481 tgcgtgatgg cgtacttacc cgcgtggaag gtgaggaacg tgtcgaagaa acgctgttga
    30541 cggtacctgg gaattatccg gcttactatg cggctattcg tgatgcgtta aatggcgatg
    30601 gtgaaaatcc ggttccggca agccaggcaa tccaggtaat ggagttgatt gagctgggca
    30661 tcgaatccgc caaacatcgc gcgactttgt gccttgcatg aaaaatggat ggtgcaattt
    30721 gcaccatcca ttttgttact tcgcggcgac tttttctcgc agtgcgcgtt tttcctcagc
    30781 gctgaggaaa gccatttcca gaccattaat ctgtgcctgg cggatttgct cgcgggataa
    30841 cccagcagct ggcgcggcaa cggtatattc gtgaatgata tccactccct gtacgccggg
    30901 atcgtcagtg ttaatgccgg cacgaatgcc atgctcaagg aacgttttca gcggatgtgc
    30961 agccagctct gctacggtgc tggtctgaat attggaggtc agacaggatt caataccaat
    31021 ttgttgctcg gcgagaaaat ccatcagcgc ccgatcttca atggctttta cgccatgtcc
    31081 aatacgctcc gcacccagtt cacgaatcgc ctgccagatg ctttccggcc cggcagcttc
    31141 gcctgcatgg acggtaatat gccagcccgc atcacgcgca aggttgaagt gagaaaggaa
    31201 cagacttccc gggaaaccaa gttcatcacc ggctaaatca agggcggtaa tctggtcacg
    31261 gtgggctaaa aaggcctcca gctcttgctg acaggcggct tcgccgaagg ttcggctcat
    31321 aatgccgata agcttcgcct gcacaccaaa agtgcggcaa ccttcacgta cgccatcgat
    31381 caccgcttcg acaacacccg ctacaggcag cttatgtgcc attgccatgt agcctggtga
    31441 aaaacgcagc tcgacatagt gcaggccgtt acgggctgca tcttcaatgt tttcaaatgc
    31501 cacgcggcgg caggcatcaa gagaggcgag aactttaacg ccccagtcaa gtttggtcag
    31561 aaagctcacc agatcgggtt cgttggcaat gacctgaacg tggggaatca gtgtttccag
    31621 ggattgtgca ggaagcgaga tattatactg gcggccaagt tcaagaatgg tctggggacg
    31681 aatgttgcca tcaaggtggc gatggatatc agttaatggc agggtggtat caatcatggt
    31741 cgcactcttt ttctggatat aaaagtgcgc accattataa aaacaaagca gggtaaaaat
    31801 ccacgattgc gcaacgattt ttacgttgcg caattggggt taacgaacag cgcggatggc
    31861 gttaattaat ccagccacac ctttttccag tttcgaacgt gggcagccgg cattgagacg
    31921 gacaaaacca cgaccttctt caccgtaggt ataccccggc atgatcgcga ctttttcttg
    31981 ttcgataagt gctttttgca acgcgttgtc gtcaatattc aacggacgta aatcaagcca
    32041 tgccagataa gtggattgtg ggatctgcca gttgagttca ggaaacgcgg cgttcatttt
    32101 atctgcgata tacgtcaggt tatctttcag atagatgcgt aaggcatcca gccacggcgc
    32161 gccttgctga taggcggcga tatgggcagt taacgccagt accgaagggg aagaaagccc
    32221 atcacggcct ttcagtgccg ataaataggc atcgcggcta ctgctatttt ctataatccc
    32281 gtaagcaccg gtcagggcgg gaatattgaa acttttcgag cccgacgtta gcaacgccca
    32341 gtctccgcga gccacattac tccagggaat atgcggctgc tcgccccaaa ccatatccat
    32401 atggatttca tcggaaataa cccgcacacc atgacgctcg cacaggtcag ccatgatctc
    32461 cagctcatcg cacgtccaca ctttcccggt aggattctgt gggctacaca ggagcataat
    32521 tttacattct ggtttcgcca acacggcttc caacttgccc atatcgcaaa accaaccatc
    32581 agcctgcttc tctaaagcaa cgggcattac tgtgcgctgg ttaccttcaa tggccttgta
    32641 aaatgcgtca taggcgggtg tgtggatcac cacgccttca cctgtttcag accactgacg
    32701 aatcagttct gaaaccatat agatgacaga agggccatac accaccgtct gagaatcgat
    32761 ggcggtgtaa tgctgggtgg aaaaccagtg ggcaatagca gcgagaaact catcgttttt
    32821 ccagcggctg tagccaaata cgccgtgcat caggcgctga ttcagcgcct ctataatgca
    32881 gggggcagtg gcaaaatcca tgtctgaaat cgtgaacggt aacaggtcag cagtgccgaa
    32941 acggtcagcg acataatccc actgtgtaca ccatgtgcca tgacgatcca cgacctttga
    33001 aaaatcgaac atatcttatc cttatgcctg gacagtatgc atcagaccgg ccatttcatc
    33061 ttttactgac tgaacttgtg gcccgataac aacctgcagg ttatgttgat taagttgtac
    33121 tacgccaatt gcccgattgt ccttcagtgc ctgcacatta acaagcgaca tatctttcac
    33181 agacaaacgc agacgggtaa tgcagttatc gaggctgaca atattgtcgg caccgcctaa
    33241 tgcttcgagg attgcaggaa cgttgtaacc tgatttaccc ggcgcagcgg caacggcttt
    33301 ttcgattgag ctggcaactt cgctatcgcg ccccggggtt ttgagattga agcgggtgat
    33361 agcgaaacgg aagatggcgt agtaaacgac aaaccagatt gccgccacca ctggcaccat
    33421 gtaccacttg gttgacagac catgcaaaat accgaacacc acgaagtcga tgatattgcc
    33481 gtcggtatta ccgatggtga cgccgagcac agacatgacg gtgaagccga ggccggttaa
    33541 cagcgcgtgg atgacataca gaactggcgc tacgaacagg aacaggaatt ccagcggttc
    33601 ggtagtgccg ccaacgacgc aggcgatcag gccagaaatc agcagacctt taattttatg
    33661 gcgattttcc gggcgcgcgc agtgatacat agctaacgct gcagctggca gaccgccgag
    33721 aaacgcaggc attttacctt gcgaaaggaa acgcgtggcg ctttcagaaa aaccgtgagt
    33781 ggtcgggcaa ctcaattgcg tctggaagat ggtcagtgcg ccgctgacgg tttgaccgca
    33841 gacttcctgc gtgccgcctg cgtcggtaaa gcgaattaat gccaccagaa tgtgatgcag
    33901 accaaacggc aacagcagac gttcaccggt accaaacagc atcggtccga aatcacccgc
    33961 gctgtttatc atatggccca agccgctaat acccatggcg aaaatcggcc agactaatgg
    34021 aatcaccagg ccgacaaggc ccatcaccag cgaggagata attggtacga agcgcgtacc
    34081 gccgaagaat gccagcgcat ccggcaggcg gatattatgg aaacgctcat gcagcatcca
    34141 gacgataata ccggcgatca ccgcaccgag gatcccggta tcgatcgact ggatcccaag
    34201 aatgctctgg atgttattgg ctttcagaac cgcggcatcc gtggttggca gaatgccttt
    34261 attggtcaac cagaagttta ccgcgaggtt cattaccgca taaccgacga agccagcgaa
    34321 tgccgctacg cctttatttt cgcgtgccag gcccagcggg atggcgatac agaacatgac
    34381 aggcaggaaa ctaaaagcaa acgagccaat cttactcatc caggtaaaga tagcctgcaa
    34441 cacggggttg cccaggaccg ggatcagggt tatgacatca tggctgctaa gagaactacc
    34501 aatgccgagc ataatgccgc agaacgacaa taatgccacg ggtaacatga aggttttgcc
    34561 taactgctgg aagaactccc acagcgtgac tttcggtgct gttttcgccg tcataaaacg
    34621 actcctcgta gagaacaatc tggattcagt aaattgcgcg agaagataaa acgttttatc
    34681 aaattttagt gaggcataaa tcacattacg caacgataat agcgggtata agataaataa
    34741 aaggtaaaac gttttatctg tcacataatc agggagtagg tcatctgcat ggctaccgcc
    34801 aaaaaaataa ccattcatga tgttgcgctg gctgcgggcg tgtcggtaag taccgtttcg
    34861 ctggtgctta gtggcaaagg gcgaatctct accgccacag gagaacgcgt taacgccgcc
    34921 attgaagagc tgggatttgt gcgcaatcgc caggcgtcgg cgctgcgcgg cgggcaaagc
    34981 ggcgtcattg gtttgatcgt ccgtgattta tctgcgccgt tttacgccga attgacggcc
    35041 ggattgacgg aagctctgga agcgcaggga cggatggttt ttttgcttca cggcggtaaa
    35101 gacggcgagc agctggcaca gcggttttca ctgttactga atcagggggt cgatggtgtg
    35161 gtaattgccg gggctgcagg aagcagcgat gacctgcgac ggatggcaga agaaaaagct
    35221 atcccggtga ttttcgcttc ccgtgccagt tatcttgatg atgttgatac ggttcgcccg
    35281 gacaacatgc aggctgcaca gttgttgacg gagcatctca ttcgcaatgg gcatcagcgg
    35341 atcgcctggc tgggagggca aagttcctca ttaacccggg cagaacgggt gggaggctat
    35401 tgtgcaactc tactaaaatt tggtctgccg tttcacagcg attgggtgct ggagtgcaca
    35461 tccagccaga agcaagctgc ggaagctatc acggcgcttt tacgtcataa cccga
//
LOCUS       DAJTOR010000021        35044 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTOR010000021 DAJTOR010000000
VERSION     DAJTOR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.021"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(72..407)
                     /locus_tag="OO249_000923"
                     /pseudo
     CDS             complement(72..407)
                     /locus_tag="OO249_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            complement(488..1915)
                     /locus_tag="OO249_000924"
     CDS             complement(488..1915)
                     /locus_tag="OO249_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5635305.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETEPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1912..2619)
                     /locus_tag="OO249_000925"
     CDS             complement(1912..2619)
                     /locus_tag="OO249_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5635306.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2782..3759
                     /locus_tag="OO249_000926"
     CDS             2782..3759
                     /locus_tag="OO249_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5635307.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3829..4311)
                     /locus_tag="OO249_000927"
     CDS             complement(3829..4311)
                     /locus_tag="OO249_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5635308.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4546..7128
                     /gene="leuS"
                     /locus_tag="OO249_000928"
     CDS             4546..7128
                     /gene="leuS"
                     /locus_tag="OO249_000928"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5635309.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7143..7724
                     /gene="lptE"
                     /locus_tag="OO249_000929"
     CDS             7143..7724
                     /gene="lptE"
                     /locus_tag="OO249_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5635310.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7724..8755
                     /gene="holA"
                     /locus_tag="OO249_000930"
     CDS             7724..8755
                     /gene="holA"
                     /locus_tag="OO249_000930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5635311.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8757..9398
                     /gene="nadD"
                     /locus_tag="OO249_000931"
     CDS             8757..9398
                     /gene="nadD"
                     /locus_tag="OO249_000931"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5635312.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9422..10030
                     /locus_tag="OO249_000932"
     CDS             9422..10030
                     /locus_tag="OO249_000932"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5635313.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPPLTARGIEQAQNLHTLLHGV
                     SFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAENY
                     SAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARLI
                     GMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVDNA"
     gene            10290..10607
                     /gene="rsfS"
                     /locus_tag="OO249_000933"
                     /gene_synonym="rsfA"
     CDS             10290..10607
                     /gene="rsfS"
                     /locus_tag="OO249_000933"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5635314.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10611..11078
                     /gene="rlmH"
                     /locus_tag="OO249_000934"
     CDS             10611..11078
                     /gene="rlmH"
                     /locus_tag="OO249_000934"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5635315.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11109..13010
                     /gene="mrdA"
                     /locus_tag="OO249_000935"
     CDS             11109..13010
                     /gene="mrdA"
                     /locus_tag="OO249_000935"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5635316.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTTHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13013..14125
                     /gene="mrdB"
                     /locus_tag="OO249_000936"
                     /gene_synonym="rodA"
     CDS             13013..14125
                     /gene="mrdB"
                     /locus_tag="OO249_000936"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5635317.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14136..15224
                     /gene="rlpA"
                     /locus_tag="OO249_000937"
     CDS             14136..15224
                     /gene="rlpA"
                     /locus_tag="OO249_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5635318.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15364..16575
                     /gene="dacA"
                     /locus_tag="OO249_000938"
     CDS             15364..16575
                     /gene="dacA"
                     /locus_tag="OO249_000938"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5635319.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16686..16949
                     /locus_tag="OO249_000939"
     CDS             16686..16949
                     /locus_tag="OO249_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5635320.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVEILYEELGKIDIVRMVL"
     gene            17050..17691
                     /gene="lipB"
                     /locus_tag="OO249_000940"
     CDS             17050..17691
                     /gene="lipB"
                     /locus_tag="OO249_000940"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5635321.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17950..18903
                     /locus_tag="OO249_000941"
     CDS             17950..18903
                     /locus_tag="OO249_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5635322.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIALNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNKEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19112..20077
                     /gene="lipA"
                     /locus_tag="OO249_000942"
     CDS             19112..20077
                     /gene="lipA"
                     /locus_tag="OO249_000942"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5635323.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20178..20381)
                     /gene="tatE"
                     /locus_tag="OO249_000943"
     CDS             complement(20178..20381)
                     /gene="tatE"
                     /locus_tag="OO249_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5635324.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20510..21298)
                     /locus_tag="OO249_000944"
     CDS             complement(20510..21298)
                     /locus_tag="OO249_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5635325.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21391..21774
                     /gene="crcB"
                     /locus_tag="OO249_000945"
     CDS             21391..21774
                     /gene="crcB"
                     /locus_tag="OO249_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5635326.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21829..22038)
                     /gene="cspE"
                     /locus_tag="OO249_000946"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21829..22038)
                     /gene="cspE"
                     /locus_tag="OO249_000946"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5635327.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22213..22773)
                     /gene="pagP"
                     /locus_tag="OO249_000947"
     CDS             complement(22213..22773)
                     /gene="pagP"
                     /locus_tag="OO249_000947"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5635328.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23362..24747
                     /gene="dcuC"
                     /locus_tag="OO249_000948"
     CDS             23362..24747
                     /gene="dcuC"
                     /locus_tag="OO249_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5635329.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24788..25468)
                     /gene="dpiA"
                     /locus_tag="OO249_000949"
                     /gene_synonym="citB"
     CDS             complement(24788..25468)
                     /gene="dpiA"
                     /locus_tag="OO249_000949"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR5635330.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25437..27094)
                     /gene="dpiB"
                     /locus_tag="OO249_000950"
                     /pseudo
     CDS             complement(25437..27094)
                     /gene="dpiB"
                     /locus_tag="OO249_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            27443..28501
                     /gene="citC"
                     /locus_tag="OO249_000951"
     CDS             27443..28501
                     /gene="citC"
                     /locus_tag="OO249_000951"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5635331.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            28516..28812
                     /gene="citD"
                     /locus_tag="OO249_000952"
     CDS             28516..28812
                     /gene="citD"
                     /locus_tag="OO249_000952"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5635332.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGTLDCILRARLEALLARASGIPTLPWEDCQ"
     gene            28809..29717
                     /gene="citE"
                     /locus_tag="OO249_000953"
     CDS             28809..29717
                     /gene="citE"
                     /locus_tag="OO249_000953"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5635333.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            29728..31260
                     /gene="citF"
                     /locus_tag="OO249_000954"
     CDS             29728..31260
                     /gene="citF"
                     /locus_tag="OO249_000954"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5635334.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIHRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQEQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            31264..31814
                     /gene="citX"
                     /locus_tag="OO249_000955"
                     /pseudo
     CDS             31264..31814
                     /gene="citX"
                     /locus_tag="OO249_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            31789..32666
                     /gene="citG"
                     /locus_tag="OO249_000956"
                     /pseudo
     CDS             31789..32666
                     /gene="citG"
                     /locus_tag="OO249_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
     gene            32717..34180
                     /gene="citT"
                     /locus_tag="OO249_000957"
     CDS             32717..34180
                     /gene="citT"
                     /locus_tag="OO249_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR5635335.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPNGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            34291..>35044
                     /gene="rna"
                     /locus_tag="OO249_000958"
     CDS             34291..>35044
                     /gene="rna"
                     /locus_tag="OO249_000958"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5635336.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQ"
BASE COUNT         8722 a         8940 c         8664 g         8718 t
ORIGIN      
        1 cccccaaacg aatcggaaaa tcgatactgg aaaaactcta actcggtggc cagtttttgt
       61 tgaccacttc acccttcccc acagcacata gcggtttggg caacgaattg ggtataaatc
      121 aaccctctct ccgagccacc agcaattaat gctttccact cttcagggga agagcgatgc
      181 catgtcagat ataagccagc taaatcattg gcatgaccat catcagtcat ctgataaata
      241 gtctgaatta aatcatcacg attatttatt ccccaggatg atgagagatc gaattctttt
      301 ataccggtat acatttttgg cgagtcatgc gcgggtttgc aaccctgatt aagtatttcc
      361 atgggcgcag aaagcgcaaa aagaagatat tgttcttcct tattcatttg ttaataatcc
      421 cttaagtgca ttaactgact acttttttat attacttgcc gggctttccg cacattaacg
      481 tcgactgtca cccgaacaga tggagtattt tccctatcaa accggcaaag ataaaaatgt
      541 agaaaataat tttgatgacg cccagacagc ccggcccacc atcgtcaacg ggcattggct
      601 gcggttgcgg ctccggttca acagtttgct tctcggtaaa attcgccgcc tgaattaata
      661 atcgacgaat aattaatgct tccagtggac aacgatcttc tgcatcaaga cggcttacga
      721 tcggcattaa tgattcttct gtcggttggc tggcctgcac taattgtttg gcaactaaat
      781 ataatggcca taagagatgt tctgtatcac gcgtgagtga gatccgcggc ttacgtcctt
      841 tgtgcaaaac catctcgacc agtgaacgcc ccagcgcaga gagtttttca tcgctgagta
      901 tttcgtataa atgaaaacgc gtacgggcgc tgtcgccggg gacataaagt agctgtttac
      961 cctgatcgtt aaccagttca cgagcttcaa tcatcatcac cactaacggg taataatcga
     1021 cccgatgctg acggcaccat tgcaatatta aagcatccgc ctgggtggag ggatactcgc
     1081 gccagtagtc acaaagttct gccagcaagc tttcgccttc tccacaatag acgcgttgcg
     1141 cgtattcata ataacgcgca tcttcattat caggttcagc ttcccgccac gaccgggcgt
     1201 aatccagaag ttcggggata tccaggcgac ctgcgctata ccagcggagt aaatttctgt
     1261 gcagttttgc atcatcgggg atgatcactg cgcgatgttg cgctaaatac aatgtcagcc
     1321 attctggatg cgatgaccaa atccgagcga acatttggta aaaatcgatg gtgtcatttt
     1381 gcacggcaac gggcagatgc agaatggtct ggaagttaaa aacattcccc ttcgcaatgg
     1441 catataaaaa ggattccagt tcttcttcgt ctatttcccc gacgttttcc tcctgcaacc
     1501 accgcaatct ttctgctaat aacaccacgc actcgaagga taaatgggca gtgttcatgg
     1561 cgattgtgca cagcgaccag cgtaattcat caatctcttc catcgagcaa taatttaatt
     1621 gctgaatgaa tcgctgccag gcggagggca gaaaacgttc gctatcagag gcaagtaacg
     1681 cgcgaaaggc gagcagaatt tcatgttctg ctacctcata ttcttctggt tgccaaacag
     1741 atttagcagg cgactgcgca atccgtagcg cctcctcata cgcctgacga agttgtttaa
     1801 aaccctgcgg ttcggtttct ggatgaaagg acggtaacag cgccagataa gcgcggcgga
     1861 taatatcgac gtcagtcgtt tcctctatat cgaggatctt ccagcaattt ttcataagtc
     1921 gctcatatcc ttaagcgttt ccgggcatgt ggggtagtca gccagtggtt gccaggacaa
     1981 ggtagggtaa tacgcatcat cactggcaaa taattgataa aacatatcat tgccggaatc
     2041 gtcgtattct ttacgcgcta agaattcgcg taataaaggc agatgatcat cttcgggaga
     2101 ctgccaatat tgccgaccaa aggtataacc ggcaaagtat tgccgccagt tactataata
     2161 acgtagagcc cgtagatgaa tacgcgactg gatccataga ctttcttctt cactcaacca
     2221 gttattaagt acgcccattc gacttaaaaa acccatccgg acataatccc atgctttaat
     2281 tcctccctca ccacagcatt cggctgtgct ggcgacaaat tgcgcataag cctgaccttg
     2341 ctcatttaat tcagcaagat agtcacgcca ttcacatgga gaataacgaa accagcggtg
     2401 ataaaaccct gccaaccgag cggcatgacc gttctcgatc attcggtgaa ttgtgcgcag
     2461 caggtcatca cggtcatcaa ttccccatga ggaagaaagg tcaaaaaagc atgtacctaa
     2521 atacattttg ggggatgaat gcgaaggtaa acagcattca ttgcgtattt ccatcggagt
     2581 ggaaagtgca aacaatattt tttgcgaccc catgtccata taatttatcc ttaaacattc
     2641 atttaatgaa tgtaaactac atttcgagac tcagttattt atacttttct gacgcgacat
     2701 aatacgcata aaataaggct tacgcaaaaa tggaatacat aaaatataat atgttttatc
     2761 tacaaccata aggaaatgat aatgattttt acgtcaagtt gctgcgataa tttatcaata
     2821 gatgagatta tcgaacgtgc tgaaaaaggc gattgcgagg ctcaatatat tgttgggttt
     2881 tattataatc gcgatagcgc aattgactct ccggacgacg aaaaggcctt ttactggctg
     2941 aagctggccg ccgagcaagg tcattgtgaa gcacaatatt ccttaggaca aaagtatact
     3001 gaggataaaa gccgtcataa agataatgag catgccatct tttggctgaa aaaaactgcc
     3061 ctacaaggcc atactttcgc ttccaacgcc cttggctgga tactggatcg tggagaagac
     3121 cccaattata aagaagcggt tgtctggtat cagatagccg cggagagcgg aatgtcttat
     3181 gcgcaaaata atcttgggtg gatgtacaga aatggcaacg gagtcgcaaa agactatgcg
     3241 ctggcatttt tctggtacaa acaagctgca ttacaaggcc atagtgacgc gcaaaacaat
     3301 ctggccgatc tttatgaaga cggaaaaggc gttgctcaaa acaagacact cgccgcattc
     3361 tggtatttga aaagcgcaca gcagggtaat cggcacgccc agtttcaaat tgcatgggat
     3421 tataacgctg gcgaaggggt ggaccaggac tataagcaag cgatgtactg gtatctgaag
     3481 gctgccgctc aggaaagcgt cggcgcttac gtcaacatcg gttatatgta taaacacgga
     3541 caaggcgttg agaaagatta tcaggctgcc tttgaatggt ttacgaaagc cgctgaatgc
     3601 aatgacgcca ctgcctggta taacctggcc attatgtatc actacggaga aggaagacct
     3661 gtcgatctcc gacaggctct cgacctgtat cgtaaagttc agtcatccgg aaccagagat
     3721 gtcagtcagg aaattcgtga gactgaagat ttactgtaga gataaaaaca agggccgggg
     3781 aatttcattc ggcccatatt ctctttattg ctgtgcgaaa tcagactatt acggctcaaa
     3841 cgggcgtctc tggaactggt catgaccaca tttcgggcat agcgtcagca cttccggtgt
     3901 gtagatcggg agatggaagt gacatttctc gcagaccaga tttcccagcc cgaccacttc
     3961 tccgctgtga taaaccccat gatgattgag gtcctggaaa acttcgcgcc attcaagctg
     4021 cgttttatcg gtgatatctg ccagctcctg ccataagctt tctttaatca cccgcataaa
     4081 gacgctgtca gattcttctt tcaggctctc ttcatagctc atggcgaact cttccaggtc
     4141 acgtcttaca gctcgcgtca gctcatcgac ctcggttcgc gttaactccc ctgtttttat
     4201 tacgcgctcg cgcgcctgtt ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg
     4261 ttcgctcagt gacgcgacca gttcacggta atattgagca accttgttca tcgtttcatc
     4321 tcccgggtcg ttaactgact ataataatag acgttctttg ttccttgctt tgctaatacg
     4381 cccacaaact ctgtaaatta atgtatgaat aatccgggca tcatttcaac gtcgagtgcg
     4441 cgaaaggctg ttttgacgcg gacgtttggg ctatgctatg cggatctgaa aaaccacctc
     4501 aacgctacat ttgtagccgt attgaaaaca ggaccactgg ctgccatgca agagcaatac
     4561 cgcccggaag agatagaatc caaagtacag cttcattggg atgagaagcg cacatttgaa
     4621 gtaaccgaag acgagagcaa agagaagtat tactgcctgt ctatgcttcc ctatccttct
     4681 ggtcgactac acatgggcca cgtacgtaac tacaccatcg gtgacgtgat cgcccgctac
     4741 cagcgtatgc tgggcaaaaa cgtcctgcag ccgatcggct gggacgcgtt tggtctgcct
     4801 gcggaaggcg cggcggtgaa aaacaacacc gctccggcac cgtggacgta cgacaacatc
     4861 gcgtatatga aaaaccagct caaaatgctg ggctttggtt atgactggag ccgcgagctg
     4921 gcaacctgta cgccggaata ctaccgttgg gaacagaaat tcttcaccga gctgtataaa
     4981 aaaggcctgg tatataagaa gacttctgcg gtcaactggt gcccgaacga ccagaccgta
     5041 ctggcgaacg aacaagttat cgacggctgc tgctggcgct gcgataccaa agttgaacgt
     5101 aaagagatcc cgcagtggtt tatcaaaatc actgcttacg ctgacgagct gctcaacgat
     5161 ctggataaac tggatcactg gccagacacc gttaaaagca tgcagcgtaa ctggatcggt
     5221 cgttccgaag gcgtggagat caccttcaac gttaacgact atgacaacac gctgaccgtt
     5281 tacactaccc gcccggacac ctttatgggt tgtacctacc tggcggtagc tgcgggtcat
     5341 ccgctggcgc agaaagcggc ggaaaataat cctgaactgg cggcctttat tgacgaatgc
     5401 cgtaacacca aagttgccga agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact
     5461 ggctttaaag ctgttcaccc attaacgggc gaagaaattc ccgtttgggc agcaaacttc
     5521 gtattgatgg agtacggcac gggcgcagtt atggcggttc cgggtcacga ccagcgcgac
     5581 tacgagtttg cctctaaata cggcctgaac atcaaaccag ttatcctggc tgctgacggc
     5641 tctgagccag atctttctca gcaagccctg actgaaaaag gcgtgctgtt caactctggc
     5701 gaattcaacg gtcttgacca tgaagcggcc ttcaacgcca tcgccgataa actgactgcg
     5761 atgggcgttg gcgagcgtaa agtgaactac cgcctgcgcg actggggtgt ttctcgtcag
     5821 cgttactggg gcgcgccgat tccgatggtg acgctggaag acggtaccgt aatgccgacc
     5881 ccggacgacc agctgccggt gatcctgccg gaagatgtgg tgatggacgg cattaccagc
     5941 ccgattaaag cagatccgga gtgggcaaaa actaccgtta acggtatgcc agcgctgcgt
     6001 gaaaccgaca ctttcgacac ctttatggag tcctcctggt actatgcgcg ctacacttgc
     6061 ccagagtaca aagaaggtat gctggattcc gaagcagcta actactggct gccggtggat
     6121 atctacattg gtggtatcga acacgccatt atgcacctgc tctacttccg cttcttccac
     6181 aaactgatgc gtgatgcagg catggtgaac tctgacgaac cagcgaaaca gttgctgtgt
     6241 cagggtatgg tgctggcaga tgccttctac tatgttggcg aaaacggcga acgtaactgg
     6301 gtttccccgg ttgatgctat cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa
     6361 gatgcggcag gccatgaact ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac
     6421 aacggtatcg acccacaggt gatggttgaa cgttacggcg cggacaccgt tcgtctgttt
     6481 atgatgtttg cttctccggc tgatatgact ctagaatggc aggaatccgg tgtggaaggg
     6541 gctaaccgct ttctgaaacg tgtctggaaa ctggtttacg agcacacagc aaaaggtgat
     6601 gttgcggcac tgaacgttga tgcgctgact gaagatcaga aagcgctgcg tcgcgatgtg
     6661 cataaaacga tcgctaaagt gaccgatgat atcggccgtc gtcagacctt caacaccgca
     6721 attgcggcga ttatggagct gatgaacaaa ctggcgaaag caccaaccga tggcgagcag
     6781 gatcgcgctc tgatgcagga agcgctgctg gccgttgtcc gtatgcttaa cccgttcacc
     6841 ccgcacatct gcttcacgct gtggcaggaa ctgaaaggcg aaggcgatat cgacaacgcg
     6901 ccgtggccgg ttgctgacga aaaagcgatg gtggaagact ccacgctggt cgtggtgcag
     6961 gttaacggta aagtccgtgc caaaatcacc gttccggtgg acgcaacgga agaacaggtt
     7021 cgcgaacgtg ctggccagga acatctggta gcaaaatatc ttgatggcgt taccgtgcgt
     7081 aaagtgattt acgtaccagg taaactcctc aatctggtcg ttggctaagc gcgggaggaa
     7141 gcgtgcgata tctggcaaca ttgttgttat ctctggcggt gttaatcacc gccgggtgtg
     7201 gctggcatct gcgtgatacc acgcaggttc cttccactat gaaggtcatg atcctggact
     7261 caggcgatcc gaacgggcca ttaagccgtg cggtgcgtaa ccagttacgt ctgaatggtg
     7321 tcgagttgct tgataaagaa accacgcgta aggacgttcc atccctgcgt ttgggtaaag
     7381 tgagcatcgc gaaagatacc gcatcggtat tccgtaacgg tcaaacagca gagtatcaga
     7441 tgatcatgac ggtgaatgcg accgtgttga tccccggccg tgatatctac ccgattagcg
     7501 ccaaagtctt ccgttcgttc tttgataacc cgcaaatggc gttagcgaaa gataacgaac
     7561 aagacatgat cgtaaaagag atgtacgacc gtgctgccga acagctgatt cgtaagctgc
     7621 caagcatccg tgctgcggat attcgttccg acgaagaaca gacgtcgacc acaacggata
     7681 ctccggcaac gcctgcacgc gtctccacca cgctgggtaa ctgatgattc ggttgtaccc
     7741 ggaacaactc cgcgcgcagc tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa
     7801 cgatcctctg ttattgcagg aaagccagga cgctgttcgt caggtagctg cggcacaagg
     7861 attcgaagaa caccacactt tttccattga tcccaacact gactggaatg cgatcttttc
     7921 gttatgccag gctatgagtc tgtttgccag tcgacaaacg ctattgctgt tgttaccaga
     7981 aaacggaccg aatgcggcga tcaatgaaca acttctcaca ctcaccggac ttctgcatga
     8041 cgacctgctg ttgatcgtcc gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg
     8101 gtttactgcg cttgcgaatc gcagcgtgca ggtgacctgt cagacaccgg agcaggctca
     8161 gcttccccgc tgggttgctg cgcgcgcaaa acagctcaac ttagaactgg atgacgcggc
     8221 aaatcaggtg ctctgctact gttatgaagg taacctgctg gcgctggctc aggcactgga
     8281 gcgtttatcg ctgctctggc cagacggcaa attgacatta ccgcgcgttg aacaggcggt
     8341 gaatgatgcc gcgcatttca ccccttttca ttgggttgat gctttgttga tgggaaaaag
     8401 taagcgcaca ttgcatattc ttcagcaact gcgtctggaa ggcagcgaac cggttatttt
     8461 gttgcgcaca ttacaacgtg aactgttgtt actggttaac ctgaaacgcc agtctgccca
     8521 tacgccactg cgtgcgttgt ttgataagca tcgggtatgg cagaaccgcc ggggcatgat
     8581 gggcgaggcg ttaaatcgct taagtcagac gcagttacgt caggccgtgc aactcctgac
     8641 acgaacggaa ctcaccctca aacaagatta cggtcagtca gtgtgggcag agctggaagg
     8701 gttatctctt ctgttgtgcc ataaacccct ggcggacgta tttatcgacg gttgatatga
     8761 aatctttaca ggctctgttt ggcggcacct ttgatccggt gcactatggt catctaaaac
     8821 ccgtggaaac gctggcgaat ttgattggtc tgacgcgggt cacaatcatc cctaataatg
     8881 ttcctccgca tcgtccccag ccggaagcga acagcgtgca gcgtaaacac atgcttgaac
     8941 tggcgattgc cgacaagcca ttatttactc ttgatgaacg cgagctaaag cgcaatgccc
     9001 cctcttacac tgcgcaaaca ctgaaagagt ggcggcagga acaaggaccg gacgtgccgc
     9061 tggcgtttat tattggtcag gattcactgc tgacctttcc gacctggtac gaatacgaaa
     9121 cgatactcga caatgcacat ttgatcgtct gtcggcgtcc aggttaccca cttgaaatgg
     9181 cgcaaccgca ataccagcaa tggctggaag atcatttgac acataaccag gaagatcttc
     9241 accttcagcc tgccggtaaa atttatctgg ctgaaacgcc gtggtttaac atctcggcga
     9301 ccatcatccg cgaacgtttg caaaacggtg aatcgtgtga ggatttattg ccggaaccgg
     9361 tactgactta cattaaccaa caaggcttgt atcgctgata cctgtcgtta cggagggcat
     9421 gatgcgactg tggttaattc gtcatggtga aacgcaagcg aatatcgatg gtctttacag
     9481 cggtcatgcg ccccccctga ccgcgcgcgg tattgagcaa gcgcaaaatc tgcatacgct
     9541 gctacatggt gtttcctttg atctggtttt atgcagtgaa ctggaacggg cacagcatac
     9601 cgcgcgactg gttctcagtg accgccagct ccccgtgcaa atcatccctg aactcaacga
     9661 aatgtttttt ggcgactggg agatgcgaca tcatcgcgac ctcatgcaag aagatgccga
     9721 aaactatagc gcgtggtgca atgactggca gcatgcaatc cccacgaacg gtgaaggatt
     9781 tcaggcattt tcgcaacgtg tggaacgctt tatcgcaagg cttagtgaat ttcagcacta
     9841 tcagaatatt ttagtcgtca gccatcaggg tgtactgagt ctgttaatcg cccgtttaat
     9901 tggcatgcct gccgaggcta tgtggcattt tcgcgttgac caggggtgtt ggagcgccat
     9961 tgatatcaac caaaaattcg cgacgctacg cgtcctcaat agccgtgcca tcggggtcga
    10021 taatgcatga cttttctgtt tttttacggg taagccgcaa cgaccattga cagacccggg
    10081 caggctgata ttctccgcag ccagactttt ttcgccagac acgactttgt agaaattgtt
    10141 ttacaaaaat ggcgatgcaa tctgcggcgc ggggtgggat gatagcccac tttcgaaagc
    10201 cgattcggcg acaattgtcc cgaaatcgcc tctggttcag gtatactgac agaccatttt
    10261 tatctatttg attcacccag ggggaaaact tgcagggtaa agcactccag gattttgtta
    10321 tcgacaaaat tgatgacctc aaaggtcagg acatcatcgc cttagacgtt caaggcaaat
    10381 ccagcatcac cgactgcatg atcatctgta cgggtacgtc cagccgtcat gttatgtcca
    10441 ttgctgacca cgttgtgcag gagtctcgcg cagcgggcct gttaccgctc ggcgtagaag
    10501 gtgaaaacag cgccgactgg attgtcgtgg atttgggcga tgtgattgtc catgtcatgc
    10561 aggaagagag ccgtcgcctg tatgaactgg aaaaactctg gagttaatgc gtgaagctgc
    10621 aacttgtcgc cgtgggaacg aaaatgccgg actgggtaca aaccggtttt accgagtacc
    10681 tgcgtcgttt tccgaaagat atgcccttcg agctgattga aattccggcc ggaaaacgcg
    10741 gcaagaatgc ggacatcaag cgcatactcg acaaagaggg tgagcagatg ttggcggccg
    10801 caggcaaaaa ccgcattgtc accctcgata ttccaggcaa gccctgggat acgccgcagt
    10861 tagccgctga gctggaacgc tggaagctgg atggtcgcga cgtcagtcta ctgattggcg
    10921 ggcctgaagg gttgtcgcct gcctgtaaag cggcggcgga gcagagctgg tcgctgtcgg
    10981 cgcttaccct cccccatccg ctggttcgcg tgctggtcgc agagagtctg taccgggcgt
    11041 ggagcatcac caccaaccat ccttatcacc gtgagtgata agggagcttt gagtagaaaa
    11101 cgcagcggat gaaactacag aactcttttc gcgactatac ggcagagtcc gcgctgtttg
    11161 tgcgccgggc gctggtcgcc tttttgggga ttttgctgct gaccggcgtg cttatcgcca
    11221 acctgtataa tctgcaaatt gttcgcttta ccgactacca gacccgctct aatgaaaacc
    11281 gcattaagct ggtgcctatc gcgcccagcc gcggcattat ttacgaccgt aatggtatcc
    11341 ctctggccct caaccgcact atctaccaga tagaaatgat gccagagaaa gtcgataacg
    11401 tgcagcaaac gctggacgct ttgcgcagcg tggttgatct gaccgatgac gatattgctg
    11461 cattccgaaa agagcgcgca cgttcacacc gtttcacctc tattcccgtg aaaactaacc
    11521 tgaccgaagt acaagtagct cgctttgccg tcaatcagta ccgttttccg ggggttgaag
    11581 ttaaaggcta taaacgtcgt tactatcctt acggttcggc gttgacccac gtcatcggct
    11641 atgtgtcgaa aatcaacgat aaagacgtcg aacgcctgaa taatgacggc aaactggcca
    11701 actatgcggc aacgcatgat atcggtaagc tgggcattga gcgttactat gaagacgtgc
    11761 tgcacggtca gaccggttat gaagaggttg aagttaacaa ccgtgggcgt gttatccgcc
    11821 agttaaaaga agtaccaccg caagccggac acgatattta cctgacgctg gatctcaaac
    11881 tccagcaata tattgaaacg ctgctggcgg gtagccgcgc agctgtggta gtcaccgatc
    11941 cgcgtacagg tggggtgctg gcgctggttt ccacgcctag ttatgaccca aacttgtttg
    12001 ttgacggtat ctccagcaaa gattattccg ccttgttgaa cgacccgaat acaccgctgg
    12061 tgaaccgcgc cacacagggg gtttatcctc ccgcgtctac agttaaaccc tatgtggcgg
    12121 tttcggcatt gagcgccggg gtgatcacgc gcaatacgac gctgtttgac ccaggctggt
    12181 ggcaactgcc aggttcggaa aaacgttatc gtgactggaa aaaatggggc cacgggcgtc
    12241 tgaatgtcac aagatcgctg gaagaatctg cggatacctt cttctatcag gtggcctacg
    12301 atatggggat cgatcgcctc tccgaatgga tgggtaaatt cggttatggt cattacaccg
    12361 gtatcgacct ggcggaagaa cgttccggca acatgcctac ccgcgaatgg aaacagaaac
    12421 gctttaaaaa accgtggtat cagggtgaca ccattccggt tggtatcggt cagggttact
    12481 ggacagcgac cccaatccag atgagtaagg cactgatgat cctgattaat gacggtatcg
    12541 tgaaggttcc gcatttgctg atgagcaccg ccgaagacgg caaacaggtg ccatgggtac
    12601 aaccgcatga accgcccgtc ggcgatattc attccggtta ctgggagctg gcgaaagacg
    12661 gtatgtacgg tgttgctaac cgccctaacg gtacgacgca taaatacttt gccagcgcac
    12721 cgtacaaaat tgcggcgaaa tctggtaccg ctcaggtctt cggtctgaaa gcgaacgaaa
    12781 cctataatgc gcacaaaatt gccgagcgtt tacgtgacca caaactgatg accgcctttg
    12841 cgccatacaa caatccgcaa gtggctgtcg ccatgattct ggagaacggc ggcgcgggtc
    12901 cggcggttgg tacgctgatg cgtcagatcc tcgaccacat tatgctgggt gataacaaca
    12961 ccgatctgcc tgcggaaaat ccagcggttg ccgcagcgga ggaccattaa tcatgacgga
    13021 taatccgaat aaaaaaacat tctgggataa agtccatctc gatcccacaa tgctgctgat
    13081 cttactggca ttgctggttt acagcgccct agttatctgg agcgccagcg gtcaggatat
    13141 tggcatgatg gagcgtaaaa tcggccaaat cgcgatgggt ctggtcatca tggtggtgat
    13201 ggcgcaaatt cctccacgcg tttatgaagg ctgggccccc tatctctata tcatctgtat
    13261 tattttgctg gtggcggtag atgctttcgg tgccatctct aaaggtgctc aacgctggct
    13321 ggacctcggt attgttcgtt ttcagccgtc ggaaattgcc aaaatagccg taccactgat
    13381 ggttgcgcgc tttatcaacc gcgacgtttg cccaccgtcg ttgaagaaca caggcatcgc
    13441 gctggtgctg atatttatgc ccacgctgct ggtggctgca cagcctgacc tgggaacatc
    13501 aatcctcgtt gcgctttccg gtctgtttgt actgttcctc tctggcctta gctggcgtct
    13561 gattggcgtc gcagtagtgc tggtggcggc gttcattcct attctgtggt tcttcctgat
    13621 gcatgattac cagcgccagc gcgtaatgat gctcctagac ccggaatcag acccactcgg
    13681 cgcgggctat cacattattc agtctaaaat tgctattggc tccggcggat tacgcggcaa
    13741 aggctggctg catggcactc agtcacaact tgaatttcta cccgaacgcc ataccgactt
    13801 tatcttcgcg gtactggcgg aagagctggg attagtgggc attttgattc tgctcgctct
    13861 ctacattctg ctgatcatgc gcgggctgtg gatagccgcc agagcgcaaa ccacctttgg
    13921 tcgcgtcatg gctggcggct taatgctgat attattcgtt tatgtcttcg taaatattgg
    13981 tatggtaagc ggtattctgc cggttgtagg ggttccgctc ccactggtca gttatggagg
    14041 atcagcgcta attgtgctga tggctgggtt cgggattgta atgtcaatcc acacccacag
    14101 gaaaatgttg tcgaaaagcg tgtaagaggt gcgcaatgcg taagcagtgg ctcgggatct
    14161 gcatcgcggc aggaatgctc gcggcatgta caagcgatga tggtcagcaa cagacggtaa
    14221 gtgtaccgca gcctgcggta tgtaacggcc ccatagttga aattagcggg gcggacccgc
    14281 gtttcgaacc actgaacgcg acggcaaatc aggattacca gcgcgacggt aaaagctaca
    14341 aaatcgtgca ggatccgtct cgatttagcc aggcgggact ggcggcaatc tatgatgccg
    14401 aaccaggcag taacctgacg gcctctggcg aagctttcga tccgacacag ctgacggcgg
    14461 cccatccaac gcttccgatc cccagctacg ccagaatcac taacctggct aacgggcgaa
    14521 tgatcgtggt gcgcattaat gatcgcggcc cttacggcaa cgaccgcgtt atttcgcttt
    14581 ctcgcgcggc agctgaccgt cttaacacgt caaacaacac caaagttcgt atcgatccga
    14641 ttattgtcgc ccaggatggt tcgctttctg gtcctggtat ggcttgtacc acagtcgcca
    14701 aacagactta cgccctgcct gcacctcccg atttaagcgg tggcgcggga acaagttcag
    14761 tgtctggccc gcagggtgac attcttccgg tcagtaattc gacgctaaaa agcgaagatc
    14821 cgaccggcgc gccggtaacc agtagcggtt tcctcggcgc accaacgacc ttagcgcctg
    14881 gtgtactgga aggcagcgaa ccgacgcctg cgccacagcc cgttgttaca gctccgtcga
    14941 caacgcctgc aacctcgcct gcaatggtga caccgcaagc cgcctcgcaa agcgccagcg
    15001 gcaactttat ggtgcaagtc ggggccgtta gcgatcaggc tcgtgcgcaa cagtaccaac
    15061 agcaactggg acagaagttc ggcgtccccg gtcgcgtaac tcaaaatggc gcggtctggc
    15121 ggatccagct tggccccttc gccagcaaag ccgaagccag taccttgcag caacgtttgc
    15181 aaaccgaagc ccaattacag tcatttatta ccaccgcgca gtagcgtaaa gcaggcatct
    15241 gatgtgtcaa tttctgtaaa tgacgttaac aaagtcacgc ggaaagtcag atgcctgccg
    15301 gtagtgcatt tgctatagta gggcactttt tttaattcca tcacggatgt cgtagttcag
    15361 accatgaata ccattttttc cgctcgtatc atgaagcgcc tggcgctcac cacggctctt
    15421 tgcacagcct ttatctctgc tgcacatgcc gatgacctga atatcaaaac tatgatcccg
    15481 ggtgtaccgc agatcgatgc ggagtcctac atcctgattg actataactc cggcaaagtg
    15541 ctcgccgaac agaacgcaga tgtccgccgc gatcctgcca gcctgaccaa aatgatgacc
    15601 agttacgtta tcggccaagc aatgaaagcc ggtaaattta aagaaactga tttagtcact
    15661 atcggcaacg acgcatgggc caccggtaac ccggtgttta aaggttcttc gctgatgttc
    15721 ctcaaaccgg gcatgcaggt tccggtttct cagctgatcc gcggtattaa cctgcaatcg
    15781 ggtaacgatg cttgtgtcgc catggctgat tttgccgctg gtagccagga cgcttttgtt
    15841 ggcttgatga acagctacgt taacgccctg ggcctgaaaa acacccactt ccagacggta
    15901 catggtctgg atgctgatgg tcagtacagc tccgcgcgcg atatggcgct gatcggccag
    15961 gcgttgatcc gtgacgtacc gaatgaatac tcgatctata aagaaaaaga atttacgttt
    16021 aacggtattc gccagctgaa ccgtaacggc ctgttatggg ataacagcct gaatgtcgac
    16081 ggcatcaaaa ccggacacac tgacaaagca ggttacaacc ttgttgcttc tgcgactgaa
    16141 ggccagatgc gcttgatctc tgcggtgatg ggcggacgta cttttaaagg ccgtgaagcc
    16201 gaaagtaaaa aactgctgac ctggggcttc cgtttcttcg aaaccgttaa cccactgaaa
    16261 gtaggtaaag agttcgcctc tgaaccggtt tggtttggtg attctgatcg cgcttcgtta
    16321 ggggttgata aagacatgta cctgaccatt ccgcgtggtc gcatgaaaga tctgaaagcc
    16381 agctatgtgc tgaacagcag tgaattgcat gcgccgctgc aaaagaatca ggtcgtcgga
    16441 actatcaact tccagcttga tggcaaaacg atcgagcaac gcccgctggt tgtgttgcaa
    16501 gaaatcccgg aaggtaactt cttcggcaaa atcattgatt acattaaatt aatgttccat
    16561 cactggtttg gttaaaaatt aaacacttga aagtgtaatt tccgtcccca tatactaagc
    16621 atcagtaaaa aaactcccgc cttctggcgg gagttgctat ttaattacgt tacgccggag
    16681 ctgacatgaa aaccaaactt aacgaactgc ttgaattccc tactcctttt acttacaaag
    16741 ttatggggca ggcgttacct gagctggttg atcaggtggt tgaagtggta cagcgccatg
    16801 cgccaggtga ctacacccca acggtaaaac caagcagcaa aggcaactac cactcggtat
    16861 ctatcactat caacgccact catatcgagc aggttgaaat actgtatgaa gaactgggca
    16921 aaatcgatat tgtccgcatg gttctgtaac tcgcttctcc gttacccggc tcctggtcgg
    16981 gtaactcccc tctcctcgct gtgatatact tttcccccac ttttactcat tctccacgga
    17041 gatgccgttt tgtatcagga taaaattctt gtccgccagc tcggtcttca gccttacgag
    17101 ccaatctccc aggctatgca tgaattcacc gatacccgcg atgatagtac ccttgatgaa
    17161 atctggctgg tcgagcacta tccggtattc acccaaggtc aggcaggaaa agcggagcac
    17221 attttaatgc cgggtgatat tccggtgatc cagagcgatc gcggtgggca ggtgacttat
    17281 cacgggccgg gacaacaggt gatgtatgtg ttgcttaacc tgaaacgccg taaactcggt
    17341 gtgcgtgaac tggtgacctt gcttgagcaa acagtggtga ataccctggc tgaactgggt
    17401 atagaagcgc atcctcgggc tgacgcgcct ggtgtctatg tcggggaaaa gaaaatttgc
    17461 tcactgggtt tacgaattcg acgcggttgt tcattccacg gtctggcatt aaacgtcaat
    17521 atggatcttt caccattttt acgtattaat ccttgtgggt atgccggaat ggaaatggct
    17581 aaaatatcac aatggaaacc cgaagcgacg actaataata ttgctccacg tttactggaa
    17641 aatattttag cgctactaaa caatccggac ttcgaatata ttaccgctta attccataca
    17701 tcaatggccc aatttacact gggtcattac tcataatacc tgctaatgct ttacatctcc
    17761 ttcgcgtttg tatattttct ttatcacttg cagaaatgag gtgccacgcc ttgctccgta
    17821 actcacccca ttgattaacc tattgaaata gttgattatg gtgagcaaaa gcgttccgtt
    17881 ccaattagac ataagattat tgtaattttt taaatattat ttttccatga ataaaattgg
    17941 agtaagtgcg tggatagtaa taatcaaatt gaaccctgtt taagcagaaa gtcgtcagaa
    18001 ggtaaaccac aaatatttac aaccttacga aatattgctc ttaaccttct gactattttt
    18061 gaagctgtat atgtacataa agggatcgtt aatgcagcga aagtgcttaa tctgcccccc
    18121 tcggcaatca gtcagtctat tcagaaactg cgcgttatat tccctgaccc attgtttatt
    18181 cgcaaaggcc aaggtgtcac tcctaccgca tttgcgatgc atctacatga gtatatcagt
    18241 cagggccttg agtccattct tggcgcgctg gatatcgaag gaagctatga taagcaacga
    18301 acgataacta ttgcgaccac tccctcggtc ggagccctgg tccttcctgt catctatcgg
    18361 gcgattaaaa ctcactatcc gcagctttta ctgcgcaacc cgcccatcag cgacgcggaa
    18421 aaccaactca gtcagtttca aaccgatctc atcatcgata acatgttttg caccaatcgt
    18481 acggtgcaac atcatgttct gttcaccgac aatatggtgt taatttgccg cgagggaaat
    18541 ccactactct ctttagaaga tgacagagag actatcgaca acgctgcgca tgtacttctg
    18601 ttaccggaag agcaaaattt cagcggtctg cggcagagag ttcaagaaat gtttccggac
    18661 cggcaaatca atttcaccag ctacaacatt ttgacaatcg ctgcactggt tgccaacagt
    18721 gacatgttag cgattattcc cagccgtttt tataacctgt ttagccgctg ctggccgctg
    18781 gaaaaactgc cttttccgtc cttaaataag gagcaaatag atttctctat ccactacaac
    18841 aaattcagcc tgcgtgatcc catcctgcac ggagtaatag atgttatccg taatgcattt
    18901 tgaaaaagta ttgttacagc cagatcacag cgcagaacga taaaatggca caatccaaca
    18961 acaattttac attttcgcga ccgctttggc tgctttcagg tccgtttcaa tgatatactg
    19021 ccagtcgtta attcaaaaat agttgataat tacaacaatc tattgaattg aaacgctttc
    19081 cttcgtaatt cgcaactgga acacgcacgc tatgagtaaa cccattgtga tggaacgcgg
    19141 tgttaaatac cgcgatgccg ataagatggc ccttatcccg gttaaaaacg tggcaataga
    19201 gcgcgaagcc ctgctgcgca agccggaatg gatgaaaatc aagcttccag cggactctac
    19261 acgtatccag ggcatcaaag ccgcaatgcg caaaaatggc ctgcattctg tctgcgagga
    19321 agcctcctgc cctaacctgg cggaatgctt caaccacggc acagcaacgt ttatgatcct
    19381 cggcgctatt tgtacccgcc gttgtccgtt ctgtgatgtt gcccacggtc gcccggtagc
    19441 tcctgatgcc aatgaaccag tgaaactggc gcagaccatt gccgatatgg cgctgcgtta
    19501 tgtggttatc acctccgttg accgtgatga cctgcgcgat ggcggtgccc agcactttgc
    19561 ggattgcatt actgccattc gggaaaaaag cccgcaaatc aaaattgaaa ctctggtgcc
    19621 ggatttccgt ggtcgtatgg atcgtgctct ggatattctg actgcaacgc caccagatgt
    19681 gttcaaccat aacctggaaa acgtaccgcg tatttaccgt caggtacggc ctggtgcaga
    19741 ttacaactgg tcgctgaagc tgctggaacg ctttaaagaa gcgcatccgg aaatcccgac
    19801 caagtctggt ctaatggtgg gactgggtga aaccaatgaa gaaattattg aggtaatgcg
    19861 cgacctgcgc cgtcatggtg tgacgatgtt aacgctgggg caatatttgc agccaagccg
    19921 ccatcacctg ccggttcaac gttacgttag cccggatgag ttcgacgaaa tgaaagccga
    19981 agcgctggcg atgggcttta cccatgctgc atgcggtccg tttgtccgtt cttcttacca
    20041 cgccgatttg caggcgaaag ggatggaagt taagtaatcc tgtaataatt acttacatct
    20101 gtctgataaa aaacccgctt tttgaagcgg tttttttgta tcgaacaaga tattgaggga
    20161 gcgtcctgct cgccacgtca ctctttatga gagagctttt cagcctgaag atcaacgtct
    20221 gcgccttttt tcgccgcagc atcatcatca ttcatcgcct tcttgaaccc tttaatggcc
    20281 gctccaaggt ctccgcccag cgtacgtaac ttcttagtcc caaacagcag aacgaccagc
    20341 gccgcaacta ctagcagttt ggtaatacta atctcaccca tagatacctt cttgacatat
    20401 aaccggcaac agaacgcgcc ttaaaaacca gagaatatta acggtcattc gacgcgcgca
    20461 cacaatagca atctgtaaca aggtgaatca agcgccgagt aaaaaaacat cataataatt
    20521 gcggcggcgc aaagcgacgg ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac
    20581 gttcgggcgt cacttccgcc ataatgagtg caggcatttc tgacgctgcc gcaatggtga
    20641 cgccaaaggg atctataatc cggctttgcc cgatattttt gttcccgcac tcgcccgccg
    20701 ccaccatata acaggtggta tccagcgcac gagcggcaag caacgttgac cagtgatgct
    20761 ctttgagcgg cccgcgaacc caggcggcag gaagtaccag gatttcagct ccctgtaatg
    20821 cctgtgccag cgccagctct ggaaagcgta agtcataaca ggtcatcaga ccgaccttca
    20881 tcccttccac ctccagtaac ggagcgattt cattaccagc atcaacacgg cgtgattcct
    20941 gaatggcaaa tgcatcatag agatgcagtt tggcataacg ggcgacgatg tttcctgcct
    21001 gaagtgccac cagcatattc catgcgcgcc ccggcgttga aggaacatga atcgtcagaa
    21061 ttgtcgtcat catgttacgt ttactttctc gccgtaaacg tccgaggaat tcgccttcca
    21121 gcagctgtgc tgatttaacc gatagatctg catcatgatc atcgcgtgcc agcaatgctt
    21181 ccggcaggac aaacagcgat acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa
    21241 tctcagcgtt cttttcccac acagatgtaa cagcaaactg tcctgcagca accagcattg
    21301 ccacctcctg tcagagtcgt aaaattcgcc aacgacgatt attcggcgtt acacttatca
    21361 ctcatacaaa tcaaatagca ggattttgca gtgttacaac ttcttttagc agtttttatt
    21421 ggcggtggta cgggaagcgt ggcgagatgg ctgttaagta tgcgatttaa cccactgcat
    21481 caggcgattc cgttggggac gctgacagca aacctgattg gggcattcat cataggaatg
    21541 ggattcgcat ggttcagcag gatgacgaac attgatccag tgtggaaagt attaatcacc
    21601 accggatttt gtggcggtct aacaaccttc tcaacatttt cggcagaagt ggtgtttttg
    21661 ttacaagagg gtcgctttgg ctgggcatta ctgaacgttt tcgtcaacct tctggggtct
    21721 tttgccatga cagcactggc tttctggctg ttttcggcct caaccgcaca ctaaagaccc
    21781 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
    21841 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
    21901 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
    21961 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
    22021 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgcgtc
    22081 ggttaccagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
    22141 aaagtcgcgt tatttttgat gttacttatt ttagctattg attttaaaga agttactaaa
    22201 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
    22261 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
    22321 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
    22381 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
    22441 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
    22501 ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt
    22561 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
    22621 atataaatca taatgttcag gctgttgcca ggttggtgca atattttctc taaacgttgt
    22681 catccactca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
    22741 ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaacat ttatcaaaaa
    22801 tctactacta gcatagcaaa gctacaatta acataacctt aatagaccca acataaagaa
    22861 taatctgaat agctgctcta tttatttccg cttgttcaag ttaaatattt ccgcgcatcg
    22921 attaaagatg atgcgtcgtt ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt
    22981 ttttgatgac aaaatgcaat caaggaaaaa ttattttatt ttttaacgag atacaacaat
    23041 caccttaaca aagtatataa tatatatcgt caacctatgc tcatattaga ttattttttg
    23101 agcgcacagc cgcagctaac accattgcaa ttaacaaatt tgcatcaatc caccatcaat
    23161 ttgcacacat tattatgtga taattgccaa ccgctaaata tgcgttttgt tatctatgta
    23221 taaaaacagt aacttcaatg tcttaatggc agtttttctt gattttaatc agcattcatc
    23281 gccaatttat tgggcatatt ttttccttaa gctttaggaa ttttttatta tttactttgg
    23341 ggcctggaga caggaaaaat tatgctgaca ttcattgaac tccttattgg ggttgtggtt
    23401 attgtgggtg tagctcgcta catcattaaa gggtattccg ccactggtgt gttatttgtc
    23461 ggtggcctgt tattgctgat tatcagtgcc attatggggc acaaagtgtt accgtccagc
    23521 caggcttcaa caggctacag cgccacggat atcgttgaat acgttaaaat attactaatg
    23581 agccgcggcg gcgacctcgg catgatgatt atgatgctgt gtggatttgc cgcttacatg
    23641 acccatatcg gcgcgaatga tatggtggtc aagctggcgt caaaaccatt gcagtatatt
    23701 aactcccctt acctgctgat gattgccgcc tattttgtcg cctgtctgat gtctctggcc
    23761 gtctcttccg caaccggtct gggtgttttg ctgatggcaa ccctatttcc ggtgatggta
    23821 aacgttggta tcagtcgtgg cgcagctgct gccatttgtg cctccccggc ggcgattatt
    23881 ctcgcaccga cttcagggga tgtggtgctg gcggcgcaag cttccgaaat gtcgctgatt
    23941 gacttcgcct tcaaaacgac gctgcctatc tcaattgctg caattatcgg catggcgatc
    24001 gcccacttct tctggcaacg ttatctggat aaaaaagagc acatctctca tgaaatgtta
    24061 gatgtcagtg aaatcaccac cactgctcct gcgttttatg ccattttgcc gttcacgccg
    24121 atcatcggtg tactgatttt tgacggtaaa tggggtccgc aattacacat catcactatt
    24181 ctggtgattt gtatgctgat tgcctccatt ctggagttcc tccgcagctt taatacccag
    24241 aaagttttct ctggtctgga agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg
    24301 gtgatgctgc tggttgccgc tggggtattc gctcaggggc ttagcaccat cggctttatt
    24361 caaagtctga tttctatcgc tacctcgttt ggttcggcga gtatcatcct gatgctggta
    24421 ttggtgattc tgacaatgct ggcggcagtc acgaccggtt caggcaatgc gccgttttat
    24481 gcgtttgttg agatgatccc gaaactggcg cactcttccg gcattaaccc ggcgtatttg
    24541 actatcccga tgctgcaggc gtcaaacctt ggccgtaccc tttcgcccgt ttctggcgta
    24601 gtcgttgcgg ttgccgggat ggcgaagatc tcgccgtttg aagtcgtaaa acgcacctcg
    24661 gtaccggtgc ttgttggttt ggtgattgtt atcgttgcta cagagctgat ggtgccagga
    24721 acagcagcag cggtcacagg caagtaaata gtaatgccgg aggcaagttt cctccggctt
    24781 tatcatgtca cccactgtgg tatatgcgtt gtggtctgcc aactttgccg tgaacaattt
    24841 cagcaataat cagatggcgg ctggcgcaat attcaagata acgtctggca gacgtgcggc
    24901 tgatggtcag tgcctgcgcc accgtttctg ccgtatgttg cacaccaggc tctttaaaca
    24961 gttttcgcac cgcgtttagc gtcaagggat caatgccggt cggcagttcg tctttaggtt
    25021 caccgcgagc ataggcatta aacatctcat caatttgctt ctggctggcg ctatcaatac
    25081 tttccagcat atgtttacgc tggcggaaac gggttagcgt ttgccccagc cgttcatagg
    25141 caatgggctt aatgagataa tcaaatacac cacaacgtac ggcttcagac accgtttcca
    25201 tatcgctggc tgcagtggta aacaccacgt cgccgggata atgcgcctgc accagttcat
    25261 gcaataaatt aatccctctg ccgtcaggaa gatagttatc gagcaagatt agccccggct
    25321 taaaacgctc gatcatcatt cgggcctgcg ccaggtttcc cgccagtaat atctgactga
    25381 atccgggaat gtgacgaata tattccgcat gcatctctgc cagcggcgtt tcgtcctcaa
    25441 cgatcaatag ggttaatgga gctgtcatta ggtttcactt tcggaatata gattgaaaat
    25501 aaggtaccgc agggatcatt atcttcgaga gtgataacac caccgcagcg cgttacgtag
    25561 ctggcaatca agtacaaccc aatgccatgt tcaccgggct cgtcagcacg cgtactgacc
    25621 ccctgctcaa atattttgtc tcgtagagac tctggaacgc cgcagccctg atcggcgact
    25681 tcaatcacca catcatcgcc ttcatcgctg aggaataatt caacgatctt gtttccttca
    25741 tcgctacgca ggctggcttc gaaggcgtta tcaagtaaat tgcccacaat ggctgcaaac
    25801 tcggtgctat ccagtcctgg cggcagttgc gaaagctggc taccggggac aatgatcatt
    25861 tttagcccca gttcccgggc gcgctgcact ttaccaaaaa gcagccccgc cacctagcga
    25921 tcggcaaacg cctcgcgcag gctgtcaata agctgttgct gggcctgaga ctccccctgc
    25981 accatcgcca gcacgcgatc atactctttc atctgcaaca gaccattgag cgtcgacatc
    26041 caattgagat gctcgtgtcg caatgtacga aggctctcaa cgtattgttt tatttgcgtc
    26101 agttgcgcat tgagggtgga aatttcgtct ttactgcgaa agctgataat ggcccccagc
    26161 aaatcatcac ctgaacgaat agcttcccga ttggcaataa cgctcagacc gttaaagtta
    26221 gccaccatat cctgacgttt ttcatcaatc tgttcggtaa agaaatcggc gggcctgacc
    26281 acttcagcaa tgggtttacc caaccattgc cgtccggggg aactcagccc cagcatcttt
    26341 cttgcgttac gattgatggc ggtaatgtaa ccgtgcggat ccaccgcaat cagcccttca
    26401 taaaccgaac taaacagcgc ctcttgctga cggaccacgc gtgcgatttg ctttggctcc
    26461 atgcccatca tctgccgacg gatatgcgcg gccaggaacc acgacagcaa catcagaatc
    26521 cctaacagca cgacaaacac acctgccatc ggtaataaaa actcagcccg ccagctatcg
    26581 attttactca ccaggtagcc aatcgacacc acgccgatga cttttccatc gtcatcaaag
    26641 attggcgttt tggcgcgcat cgccatcccc attgaccctt tcccggtaat gaagtagctc
    26701 tcccctttct ccagcgcgcc ctgtttggtg aactgcatag gataaccaat tttctccgga
    26761 ttaggatggt aaaggcggat cgagtgccgg tcgccatcac cacataatca aaatcggtat
    26821 ctctttgtaa tttgttagcg atggtcgcca gccgtttgta gtcacgcgtt ttcaccgcag
    26881 agatgacact gtcattggag gcaataattt tcgcctgatt cattgccatg tcgcgtacat
    26941 gaagcgttaa atagtcctca aaactggccg taaaatattg cgccaaggct gcaatgacaa
    27001 atattgagaa caccagaatc agcaaaaaga tacgcagcgg aaatgccagt ctttggaaaa
    27061 atgcaaactg tttattctcg ttaagctgca acattgtttt ccttgcgggt tatcccgagc
    27121 ctctttgaca gatgtaatta aaatgttgaa tcaaaataca tagatattaa ttatgatcgt
    27181 ggcaaatttt attcattaaa tcaattaaat cacttaaacc tgcaggtcat ttcgtaatta
    27241 cattaattaa aaaacttaaa ccattaaata aataaaaatt tcacgccgaa atactcctta
    27301 ggatgtatag cgaaaggaga aaaagatata cctcgatcac ccccctttct cccaagtgaa
    27361 aataaaaggt tatcagtttg caacattgaa caacattcgt tgcaaatcga taacaacatg
    27421 caccttcagg atactattta ttatgttcgg caatgatatt ttcacccgcg taaaacgttc
    27481 agaaaataaa aaaatggcgg aaatcgccca attcctgcat gaaaatgatt tgagcgttga
    27541 caccacagtc gaagtattta ttaccgtaac ccgcgatgaa aagcttatcg cgtgcggtgg
    27601 aattgccgga aatattatta aatgcgttgc tatcagtgaa tccgttcgcg gtgaaggact
    27661 ggcgctgaca ttagccactg aactgataaa cctcgcctat gagcggcaca gcacgcatct
    27721 gtttatttat accaaaaccg aatacgaggc gctgttccgc cagtgcggtt tttccacgct
    27781 gaccagcgta cccggcgtga tggtgctgat ggaaaacagc gccacgcgac tgaaacgcta
    27841 tgccgaatcg ctgaaaaaat ttcgtcatcc agggaacaag attggctgca ttgtgatgaa
    27901 cgccaatccc tttacgaatg gtcaccgtta tctgattcaa caagctgcgg cacagtgcga
    27961 ctggttgcat ctgtttttag tcaaagaaga ttcttcacgc ttcccctatg aagaccggct
    28021 ggatctggtg ttaaaaggca ccgccgatat tccacgcctg actgtgcatc gcggctccga
    28081 atacatcatc tcccgcgcta cgttcccttg ctacttcatt aaagaacaga gcgtcattaa
    28141 ccattgttac accgaaattg atctgaagat tttccgtcag tacctcgctc ccgcactggg
    28201 tgtaactcac cgctttgtcg gtactgaacc cttttgtcgc gttaccgccc agtacaacca
    28261 ggatatgcgc tactggctga aaacgccgac tatctccgca ccgcccatcg aactggttga
    28321 aattgagcgg ctgcgttacc aggagatgcc gatatccgct tcccgggtac gtcaactgct
    28381 ggcgaaaaac gatctcacgg ctatcgcgcc gctggtccct gcagtcacgc tgcattattt
    28441 gcagaacctg cttgagcact cccgccagga cgcggcagct cgtcaaaaga cccccgcatg
    28501 agaaacaggt gaaaaatgaa aataaaccag cccgccgttg caggcaccct tgagtctggg
    28561 gatgtgatga tacgcatcgc cccactcgat acgcaggata tcgacctgca aatcaacagc
    28621 agcgttgaga aacagtttgg cgatgcaatt cgcaccacca ttctggacgt tctcgcccgc
    28681 tacaacgtgc gcggcgtaca gctgaatgtc gatgacaaag gcacactgga ctgcatttta
    28741 cgtgcacgac tggaagccct gctggcacgc gccagcggta tcccgactct gccatgggag
    28801 gattgccaat gatttccgct tcgctgcaac aacgtaaaac tcgcacccgc cgcagcatgt
    28861 tgtttgtgcc tggtgccaat gccgcgatgg tcagcaactc cttcatctac ccggctgatg
    28921 ccctgatgtt tgacctcgaa gactccgtgg cattacgtga aaaagacacc gcccgccgca
    28981 tggtttatca cgcgctgcaa catccgctgt atcgcgatat tgaaaccatt gtgcgtgtca
    29041 acgcgctgga ttccgaatgg ggtgttaacg acctggaagc cgtcgttcgc ggtggtgcgg
    29101 acgttgtgcg tctgccgaaa accgataccg ctcaggatgt tctggatatt gaaaaagaga
    29161 tcctgcgtat cgaaaaagcc tgtggtcgtg aacccggcag caccggcctg ctggcggcga
    29221 ttgaatctcc gctgggcatt acccgcgcag tggaaatcgc tcacgcttcc gagcgtttga
    29281 tcggtatcgc cctcggtgca gaagactatg tgcgcaacct gcgtacagaa cgctccccgg
    29341 aaggaactga actgctgttc gcacgctgtt ccattttgca ggccgcgcgc tctgcgggta
    29401 ttcaggcgtt cgataccgtc tattccgacg ctaacaacga agccggattc ctgcaagaag
    29461 ccgcccacat caaacagctg ggctttgacg gcaaatcgct gatcaacccg cgtcagattg
    29521 atctgctgca caacctctac gcaccgaccc agaaagaagt ggatcacgcc cgccgcgtcg
    29581 tagaagccgc tgaagccgcc gctcgcgaag gcctcggcgt ggtttccctg aacggcaaga
    29641 tggtggacgg tccggttatc gatcgcgccc gtctggtgct ctcccgtgca gaactttccg
    29701 gcatccgcga agaataaggc aatcaaaatg acgcagaaaa ttgaacaatc tcaacggcaa
    29761 gaacgggtag cggcctggaa tcgtcgcgct gaatgcgatc ttgccgcttt ccagaactca
    29821 ccaaagcaaa cctaccaggc tgaaaaagcg cgcgatcgca aactgtgtgc caacctggaa
    29881 gaagcgattc atcgctctgg tttacaggac ggcatgacgg tttccttcca tcacgctttc
    29941 cgtggcggtg acctgaccgt caatatggtg atggacgtca tcgcgaagat gggctttaaa
    30001 aacctgactc tggcctccag ctccctgagt gattgccatg cgccgctggt agaacacatt
    30061 cgccagggcg tggttacccg catttatacc tctggcctgc gcggtccact ggcggaagaa
    30121 atctcccgtg gtctgctggc ggaaccggtg caaatccact ctcacggcgg acgtgtgcat
    30181 ctggtacaga gcggcgaact gaatatcgac gtggctttcc tcggcgtccc atcctgtgat
    30241 gaattcggta atgccaacgg ttacaccggt aaagcctgct gtggctccct cggctatgca
    30301 atggttgatg ccgacaacgc aaaacaggtc gtgatgctta ccgaagaact gctgccttat
    30361 ccgcataatc cggcaagcat tgagcaagag caggttgatt tgatcgtcaa agttgatcgc
    30421 gttggcgatg ctgcaaaaat cggcgctggt gcgacccgta tgaccactaa cccgcgcgaa
    30481 ctgcttattg cccgtagcgc tgcggatgtg attgtcaact ctggctactt caaagaaggt
    30541 ttctccatgc aaaccggcac cggcggcgct tcgctggcgg taacccgttt cctggaagac
    30601 aaaatgcgta gccgcgatat tcgcgccgac ttcgccctcg gcggtattac tgcaacgatg
    30661 gttgatctgc acgaaaaagg tctgatccgc aaactgctgg atgtgcagag ctttgacagc
    30721 catgctgcgc aatcgctggc ccgtaacccc aatcacatcg aaatcagcgc caaccagtac
    30781 gctaactggg gttcgaaagg cgcatcggtt gatcgtctcg acgtggtggt actgagcgcg
    30841 ctggaaattg acacccagtt caacgttaac gtgctgaccg gctctgacgg cgtactgcgt
    30901 ggtgcttccg gtggtcactg cgataccgcg attgcctctg cgctttccat catcgtcgcg
    30961 ccgctggtac gcggtcgtat tccgactctg gtggataacg tgctgacctg catcaccccg
    31021 ggctccagtg tcgatattct ggtcacagac cacggtatcg cagttaaccc ggcacgtccg
    31081 gagctggcag aacgtctgca ggaagcgggc attaaagtgg tttccattga gtggctgcgt
    31141 gaacgtgcgc gtctgctgac cggtgaacca cagccgattg aattcaccga ccgcgtcgtt
    31201 gccgttgtgc gttaccgcga tggctcggtg atcgatgttg tacatcaggt gaaggaataa
    31261 gccatgcacc tgcttcctga actcgccagc caccatgcgg tgtcaattcc cgagctgctc
    31321 gtcagccggg atgaaaggca agcacggcaa cacgtctggc tcaagcgcca tcctgttcca
    31381 ctggtctcct ttaccgtggt tgcgcctagg ccgattaaag acagtgaggt cacacgccga
    31441 atttttaatc atggcgtgac agccttgcgt gccttagccg caaaacaggg ctggcaaatt
    31501 caggagcagg ctgcactggt ttccgccagc gggccggagg gtatgttgag cattgccgcc
    31561 ccggctcgcg acctcaagct cgccaccatt gagcttgaac atagtcatcc tctcgggcgg
    31621 ttatgggata tcgatgtcct gacgcccgaa ggcgaaattc tctcccgccg cgactattca
    31681 ctgccgcctc gccgctgcct gttgtgcgag caaagcgcag ccgtctgcgc gcgtggaaaa
    31741 cccatcaact gaccgattta ctcaaccgca tggaggcact gctgaacgat gtcgatgcct
    31801 gcaacgtcaa ctaaaaccac aaagcttgcg acgtcattaa tcgatgagta cgccctgctg
    31861 ggctggcacg ccatgctgac tgaagtcaat ctgtcaccga aaccaggtct cgtggatcgc
    31921 attaactgcg gtgcgcataa agatatggcg ctggaagatt tccaccgcag cgcgctggcg
    31981 attcagggct ggctaccccg tttcattgaa tttggtgcct gtagtgcgga aatggcacca
    32041 gaagcggtac tccacggatt acgcccaatt ggtatggctt gcgaaggtga tatgttccgc
    32101 gccaccgcgg gcgtaaacac gcataagggc agcatttttt ctttagggct actgtgtgcg
    32161 gcaattggcc gtttgcttca gctcaaccag tcgctaacgc caacaaccgt ttgttctacg
    32221 gcggcaagtt tctgccgtgg cctgaccgat cgcgaactgc gtaccaataa ttcacaactg
    32281 acggcaggtc aacggttgta ccagcagctt gcccttaccg gcgcacgcgg tgaagccgaa
    32341 gcgggttatc cactggtgat caaccacgcc ttgccgcatt acctcactct gctggatcag
    32401 gggttagatc ctgaactggc attgctcgat accttgctcc tactgatggc gatcaacggc
    32461 gataccaacg ttgcttcgcg cggtggcgag ggggcctgcg ctggctacag cgcgaggcgc
    32521 aaacattatt gcaaaaaggg ggcattcgaa cccccgccga tctcgattat ctccggcagt
    32581 tcgacaggga gtgtatcgaa cgaaatctca gtctaggcgg cagtgccgac ctgctgatcc
    32641 ttacctggtt tttagcacag atttaattat ttaagcactt gataaatttg gaaatattaa
    32701 ttttcggaga acccgtatgt ctttagcaaa agataatata tggaaactat tggccccact
    32761 ggtggtgatg ggtgtcatgt ttcttatccc tgtccccaac ggtatgccac cacaggcatg
    32821 gcattacttc gctgtgtttg tggcaatgat tgtcggcatg atcctcgagc caattccggc
    32881 aacagcgatc agttttattg cggttactat ttgcgttatt ggcagtaatt acctgctctt
    32941 tgatgccaaa gaattagctg acccagcgtt taatgcgcaa aaacaggcgc tgaaatgggg
    33001 tctggctggt ttttccagca ccacggtatg gctggtattt ggcgcattta tttttgcatt
    33061 agggtatgaa gtttccgggt taggtcgtcg cattgccctt ttcctggtga aattcatggg
    33121 caaacgcacg ctgacgttgg gttatgcgat tgtcattatc gacattctgc tggcgccgtt
    33181 tacaccgtcc aacaccgcgc gtaccggggg tacggttttt ccggtcatta aaaacctgcc
    33241 gccgttgttt aaatcattcc ccaacgatcc gtccgcgcgt cgtattggcg gctatttgat
    33301 gtggatgatg gtcattagta ccagtctgag ttcgtccatg tttgtcaccg gtgcggcacc
    33361 aaacgtgctg ggtctggagt tcgtcagcaa aattgccggt atccagatta gctggttgca
    33421 gtggttcctc tgcttcctgc cggttggggt tatcttgctt atcattgcgc cgtggctttc
    33481 ctacgtgctg tacaaaccgg aaatcacaca cagtgaagaa gtggcaacct gggcgggtga
    33541 tgaactgaaa accatgggtg cgctgacacg cagagagtgg acactgattg gccttgtatt
    33601 gctcagctta ggtttgtggg tttttggcag tgaagtcatt aatgctactg cagttggtct
    33661 gctggcagtt tcgctaatgc tggccctgca cgttgtgcct tggaaagata ttacccgcta
    33721 taacagcgca tggaacacgc tggtcaacct ggcaactctg gttgtgatgg ctaacggcct
    33781 gactcgttct ggctttattg actggttcgc cggtaccatg agtacgcacc tggaaggatt
    33841 ctcaccaaac gcaacggtga ttgtactggt tctggtgttc tactttgcac actacctgtt
    33901 tgccagcctg tctgcgcaca ccgcaaccat gctgccggtt attctggccg tcggtaaagg
    33961 tattccgggc gtaccaatgg aacaactgtg tatcctgctg gtgctgtcta tcggtatcat
    34021 gggctgtctg acgccctatg caaccggtcc tggggtgatt atttacggct gtggctatgt
    34081 gaaatcaaaa gattactggc gtcttggcgc aatcttcggg gtgatttata tctctatgtt
    34141 gctgttggtt ggctggccga ttctcgccat gtggaactaa tacgttcgcc aaacgctgaa
    34201 taaatcatca cgcccgccag gtaagcccac ctggcgggcg tgatgattta atagatagta
    34261 ttcagtgtat gtatgagttc cacacccatt atgaaagcat tctggcgtaa cgccgcgttg
    34321 ctcgcggttt ctctgcttcc cttctcttct gccaacgccg tagcgttgca ggcaaaacag
    34381 tatggcgatt ttgatcgcta tgtcctggcc ctctcctggc aaaccggatt ttgccagagt
    34441 caacacgatc gaaatcgtaa cgaacgagat gaatgtcgcc tgcaaaccga aacgaccaac
    34501 aaagctgatt ttctgaccgt acatggtctg tggccaggat tgcctaaatc ggttgctgcc
    34561 cgtggtgttg atgaacgccg ctggatgcgc ttcggttgcg ctactcgccc aatcccgaat
    34621 ctaccagaag cgcacgccag ccggatgtgt tcatcgccgg aaaccgggtt atcactggaa
    34681 acggccgcta aactaagtga agtcatgcca ggagctggcg gacgttcctg cctggaacgc
    34741 tacgaatatg ccaaacacgg tgcctgcttt ggttttgatc cggacgcata cttcggtacg
    34801 atggtacgcc tgaatcaaga aattaaagag agcgaagccg gaaaattcct tgcggataat
    34861 tacggtaaaa cagtgagccg ccgtgacttt gacgccgcct ttgccaaaag ctggggaaaa
    34921 gagaacgtga aagcagttaa gctaacgtgc cagggtaacc ctgcgtattt gactgaaatt
    34981 cagatctcga tcaaagctga cgccatcaac gctccgcttt ctgcaaactc attcttgcca
    35041 caac
//
LOCUS       DAJTOR010000022        35011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTOR010000022 DAJTOR010000000
VERSION     DAJTOR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.022"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            32..1270
                     /gene="dsdA"
                     /locus_tag="OO249_000959"
                     /pseudo
     CDS             32..1270
                     /gene="dsdA"
                     /locus_tag="OO249_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OO249_000960"
     CDS             complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OO249_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5635337.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OO249_000961"
     CDS             complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OO249_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5635338.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYVYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            4495..5109
                     /gene="evgA"
                     /locus_tag="OO249_000962"
     CDS             4495..5109
                     /gene="evgA"
                     /locus_tag="OO249_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5635339.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGASAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            5114..8707
                     /gene="evgS"
                     /locus_tag="OO249_000963"
     CDS             5114..8707
                     /gene="evgS"
                     /locus_tag="OO249_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5635340.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OO249_000964"
     CDS             complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OO249_000964"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5635341.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDYDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OO249_000965"
     CDS             complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OO249_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5635342.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OO249_000966"
     CDS             complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OO249_000966"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5635343.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSSVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OO249_000967"
     CDS             complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OO249_000967"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5635344.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(14507..15139)
                     /locus_tag="OO249_000968"
     CDS             complement(14507..15139)
                     /locus_tag="OO249_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5635345.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            15435..15710
                     /locus_tag="OO249_000969"
     CDS             15435..15710
                     /locus_tag="OO249_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635346.1"
                     /translation="MKVNLILFSLFLLAYIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(15787..16029)
                     /locus_tag="OO249_000970"
     CDS             complement(15787..16029)
                     /locus_tag="OO249_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5635347.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTEIFMFLAPACGLMIRFMVGYGRR"
     gene            16382..17302
                     /gene="lpxP"
                     /locus_tag="OO249_000971"
     CDS             16382..17302
                     /gene="lpxP"
                     /locus_tag="OO249_000971"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5635348.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            17658..17729
                     /gene="ypdK"
                     /locus_tag="OO249_000972"
     CDS             17658..17729
                     /gene="ypdK"
                     /locus_tag="OO249_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5635349.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OO249_000973"
     CDS             complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OO249_000973"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5635350.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRQDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            19409..21105
                     /gene="ypdA"
                     /locus_tag="OO249_000974"
                     /pseudo
     CDS             19409..21105
                     /gene="ypdA"
                     /locus_tag="OO249_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
     gene            21120..21854
                     /gene="ypdB"
                     /locus_tag="OO249_000975"
     CDS             21120..21854
                     /gene="ypdB"
                     /locus_tag="OO249_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5635351.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            21867..22724
                     /locus_tag="OO249_000976"
     CDS             21867..22724
                     /locus_tag="OO249_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5635352.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNSQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22727..25222)
                     /gene="ptsP"
                     /locus_tag="OO249_000977"
     CDS             complement(22727..25222)
                     /gene="ptsP"
                     /locus_tag="OO249_000977"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5635353.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRESDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFCTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(25247..26284)
                     /gene="ypdE"
                     /locus_tag="OO249_000978"
     CDS             complement(25247..26284)
                     /gene="ypdE"
                     /locus_tag="OO249_000978"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5635354.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGTRSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGVRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            complement(26284..27369)
                     /gene="ypdF"
                     /locus_tag="OO249_000979"
     CDS             complement(26284..27369)
                     /gene="ypdF"
                     /locus_tag="OO249_000979"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5635355.1"
                     /translation="MTLLASLRDWLKEQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSSYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(27384..28631)
                     /locus_tag="OO249_000980"
     CDS             complement(27384..28631)
                     /locus_tag="OO249_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5635356.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGVIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(28653..28979)
                     /locus_tag="OO249_000981"
     CDS             complement(28653..28979)
                     /locus_tag="OO249_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5635357.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(29198..30163)
                     /gene="glk"
                     /locus_tag="OO249_000982"
     CDS             complement(29198..30163)
                     /gene="glk"
                     /locus_tag="OO249_000982"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5635358.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            30367..31623
                     /locus_tag="OO249_000983"
     CDS             30367..31623
                     /locus_tag="OO249_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5635359.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTINIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            31738..32064
                     /locus_tag="OO249_000984"
     CDS             31738..32064
                     /locus_tag="OO249_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5635360.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERSYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(32205..33443)
                     /locus_tag="OO249_000985"
     CDS             complement(32205..33443)
                     /locus_tag="OO249_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5635361.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKTLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVLPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            33779..34981
                     /gene="nupC"
                     /locus_tag="OO249_000986"
     CDS             33779..34981
                     /gene="nupC"
                     /locus_tag="OO249_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5635362.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         9233 a         8695 c         8081 g         9002 t
ORIGIN      
        1 gtcacggcga actgcctttc aaggttgaag tggcacgacc tcattggctg aaggtttacc
       61 ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc
      121 accctatctg gcaaaagcat ttgctgaaac tgctgccact ggggggatta ttgaatcaga
      181 actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag
      241 cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccagctcca taaaagcacg
      301 cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag cggggttgct
      361 gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca
      421 atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc
      481 ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc
      541 gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt
      601 cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa
      661 ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag cgcaatttgc
      721 ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt
      781 tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca
      841 ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt
      901 acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct
      961 tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gtgcgtctgc tggatggctt
     1021 ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg
     1081 tattcgtctt gaaccttcgg cactggcggg tatggccgga cctcagcgcg tgtgtgcatc
     1141 agtaagttac caacagatgc acggtttcag cgcagaacaa ctgcgtaata ccactcatct
     1201 ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa
     1261 aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga gcgatgctgc
     1321 cgatggcgca gacttaagat ccccggtctt acccgctata accccctcct tttatcatca
     1381 cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg taagtaagat
     1441 aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt
     1501 tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccataatatt tatccataat
     1561 ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg
     1621 gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga
     1681 caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc
     1741 tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctgaaaaaa
     1801 ctgcggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta
     1861 atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt ctattttgtt
     1921 gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg gcgcataagc
     1981 aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg gcataaggac
     2041 gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa tggtgaagtt
     2101 acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact cccaaatgac
     2161 taaagagatc agagaaataa ctgatactac tgttaatatt attattgtac tcgagttgaa
     2221 ccaatccaga tcgcgccctt tatcaagcat aatttgcaag ccaccaacac cgagcactaa
     2281 cagggtcagt cctggtagat tcattttgac cggtgaagtc tcagtttctc ttcctttaag
     2341 taaggttaag cataatgtca ggacgataat ccccataggg acattgatta aaaatatcca
     2401 accccagcta aagttatcac aaatataacc gcccaatatc ggcccacata tcggagcgat
     2461 aatcacggtc attgaccata atgccagagc aaatgttctt ttttctggcg gataattcct
     2521 taataataaa ctctgtgaca gtggaattaa cggccccgcc attaacccct gaacgactct
     2581 aaaaaatatc agcacatcaa gattggtcga taggctacac attaatgaag acagcgaaaa
     2641 aaaggttact gaaagtaaaa ataatcttaa ttcgcctatt ctttgtgcca acctgccagt
     2701 aacagggatc gcaatggcat ttgctacacc aaacgaggtg ataacccagg tgccttcgtc
     2761 tgttgatgct cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc
     2821 caacatttgc ataaatgtcg ctaatgacaa tgcaatagtg acgcaccata acgtcccacc
     2881 ggttaatggt gccggagtcg atttagtgat tgccattaaa gttgtccatt atgcgaaata
     2941 atgttgctaa tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa
     3001 gccggcatgg aggtcacggt tgaagccagc tcaggcatct cggcaatgtc ttcgttcttc
     3061 gtatcaatag ttgctgtcat cgataaacca atacgcaagg gatgttccat gagttctttt
     3121 ggatcaagag aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca
     3181 ttttgtgcag gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga
     3241 ccatgaaaca caacattttc accataaaga tcgctgataa tattgaccga ttgaccaatc
     3301 cgcacatccg tgagttgtgt ttctttaaag ttggcattaa cccacatttg acgtgccggt
     3361 acgacagcca ttaacgattg tccggggctc actgtttcgc cgacctgaac acttctctgg
     3421 gcaatatagc cggtaaccgg actcttaata tccgtacgtt taagcgccaa ccaggcttct
     3481 ttagttgcat ccgccgcttc aacgacttgt ggctgacggt ttaatggtgt gttcattact
     3541 aaagctttat tcgctttata agcctggata gcggcattca atgccgcttt gctacttatt
     3601 aacgtatctt tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaacggc
     3661 actcgacggt tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg
     3721 gcactgtatt gtttatcctg taagtatagt ttattcgttt gccgaacaat atttgccaga
     3781 ttatttttag ctttattgag tgcgatagtg gcatcagttt tatccagtga aactaaaatg
     3841 tcaccttgtc gaacgtagtt cgtatcttta tgattaacga cagtgacact acctgagact
     3901 tgtgcagaaa ttggatctgc attccccgtg acataggcgt catctgtact aatcatgtct
     3961 tctaattcca ttgaccaata gacataggca cctgaaaacg caataaataa aactaccgcc
     4021 aataaagaaa agtattttct tctgttagaa tgttttttat ttgaattaat ctgttccact
     4081 attatctctc atttctcata gatgaaattt atgaattgaa tgaaaagagc attcattctt
     4141 catatcaaac acagtgcatg tgttgcacat tatcccagga acgttggagg cctaatttaa
     4201 atgaggtgca aaaaaaacaa cagaggacta aaccgtggct tttgcaatac aattcttacg
     4261 cctgtaggat tagtaagaag acttatagtg ctaacttgaa actataaatc atcggtacaa
     4321 cccctgattt tattgttgac atttcattta tgccgactat ttatatggta tacttgtcga
     4381 attatcttaa aggaagctca gattttctta tttttattga gaaaatgaga tgacgcctta
     4441 tgtctgtatt actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac
     4501 gcaataatta ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa
     4561 aacgatattg aaatcttagc ggagttgact gaaggggcaa gcgccgttca gcgggtggaa
     4621 acacttaagc ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag
     4681 gtgttagaaa cgctgaggaa gcgccaatat agcggaatta ttattattgt ctccgctaaa
     4741 aatgaccatt tttacgggaa acattgtgct gatgctggcg ctaatggatt cgtgagtaaa
     4801 aaagaaggca tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat
     4861 ttccccttct ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac
     4921 tccttatcga aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat
     4981 gacattgctg aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcctg
     5041 atggaaaaat tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa
     5101 atcggctaat cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc
     5161 gaccataagt ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg
     5221 tgtcacactt gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa
     5281 aaatcttgtg attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca
     5341 gcaacgggtt cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat
     5401 caaattaaca ctccgggaat acgccgatca tcaaaaagca atggacgcgc tggaagaagg
     5461 tgaagtcgat atagtgttat cacatttagt tgcttcgccg cctcttaatg atgacattgc
     5521 tgcaaccaaa ccactgataa ttacctttcc ggcgctcgtc accacccttc acgattcaat
     5581 gcgaccgctt acctcatcaa aaccagtaaa tattgctcga gtagcaaatt atccccctga
     5641 cgaggtaatt catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca
     5701 ggcattagca tccgtctcag ccggacagaa tgattacttt attggtagta acatcattac
     5761 cagcagtatg atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc
     5821 accgcgtcaa tataattttt tcttgaccag aaaagaatct gtcgttctta atgaagtact
     5881 caatcgattt gttgatgctt taacaaatga agttcgctat gaagtttcac aaaattggct
     5941 tgatacagga aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca
     6001 gtggattaag caacatcccg atttaaaggt gctggaaaat ccttactctc ctccctattc
     6061 tatgacggat gaaaatggct cggttcgggg cgttatgggg gatattctta atattattac
     6121 cttgcaaaca ggtttaaatt tttctccgat caccgtttca cacaatatcc atgccggaac
     6181 acagcttaac cccggtggat gggatataat acctggcgct atttatagtg aagatcgaga
     6241 aaataatgtt tcatttgcag aagctttcat aacaacgcca tacgtttttg tcatgcaaaa
     6301 agcacctgac agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta
     6361 tgagctgcat tcgcaattaa aagagatgta tccggaggtt gagtggataa aagtcgataa
     6421 cgccagcgct gcatttcaca aggtcaagga aggtgaactt gatgctctgg tcgcgacaca
     6481 gctaaattcg cgttacatga tcgatcatta ctatcctaat gaactgtatc attttcttat
     6541 tcccggcgtt ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa
     6601 ggatattatt aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga
     6661 aaaatggatt aaaatgccca atgtgaccat tgacacatgg gacctctata gcgagcaatt
     6721 ttatattgtt acgacattat ccgttttatt agttggcagt agccttttat ggggattcta
     6781 cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc
     6841 attccgaaaa gcactctcgg attccttacc aaatccaact tatgttgtaa actggcaagg
     6901 taatgtcatt agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa
     6961 tgcaatgtta ccattagaaa acagtgactc accctttaaa gatgtttttt ctaatgcgca
     7021 tgaagtcaca gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga
     7081 taatggcatc gagaaaagat gcattaatca ctggcataca ttatgcaatc tgccagccag
     7141 cgatcatgct gtttatattt gtggttggca agatattacc gagacgcgtg atttaattca
     7201 tgcactcgaa gtagagagaa ataaagcgat caatgcaact gtcgcaaaaa gtcagtttct
     7261 ggcaacaatg agtcacgaaa taagaacacc aataagctcc attatgggct tcctggaact
     7321 tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc
     7381 caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc
     7441 gggtaactat caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg
     7501 tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt
     7561 tcctgatcat tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt
     7621 actgagcaat gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggt
     7681 tcacattgat gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt
     7741 atcgcaggaa gaacaacaac aactctttaa acgctacagc caaacaagtg caggtcgcca
     7801 gcaaacagga tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg
     7861 cgatttgtca ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt
     7921 agaaattagc cagcaagtgg cgactgtcga ggcaaaagca gaacacccca tcacactacc
     7981 tgaaaagttg agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg
     8041 ccagctaaat ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca
     8101 caaagtcagt atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga
     8161 tggttttgag ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct
     8221 tacagccaac gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg
     8281 tttgttcaaa ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt
     8341 tgcgcatatt gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc
     8401 gaacgatcta caactgatgc aggagattct catcactttc cagcatgaaa cgcataaaga
     8461 tctacccgct gcgtttcaag cactagaagc tggcgataac agaactttcc atcagtgtat
     8521 tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca
     8581 tcagttagaa ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa
     8641 ctctgtaaaa gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa
     8701 tgactaaata gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac
     8761 atttatgatg agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc
     8821 ggcataacat gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt
     8881 cccccggctt caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc
     8941 acacttaata acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca
     9001 gctgcctgcg tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg
     9061 cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg
     9121 cataacgcag aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta
     9181 ttgaaaacat caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc
     9241 cctgtcgcga tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc
     9301 gctatatcga catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt
     9361 ccgctgaata aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc
     9421 ggagcatcag gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg
     9481 taggcaggag catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg
     9541 aggcgcgggt tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct
     9601 gggcgaaaat tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta
     9661 tcgtaatcat tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa
     9721 tagagtgact gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc
     9781 ggctcaactt taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca
     9841 ttaaggacat gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta
     9901 tttgtcattt gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg
     9961 taaaatcaat aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc
    10021 cgtgacgaca aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac
    10081 attaaaccgg ctggcaataa taattccgga gaatgccggc ggtaatgcgc ctgccagtac
    10141 catcatctgc agatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagtgccag
    10201 tggcatcaga atcagcttca ggaaggtgtt ataagcaatt tcggcactga actcgaattt
    10261 atgtgcagcc agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat
    10321 cagattaaag gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt
    10381 tgccagaaca ggtgcccata ctactggctc ctttgccgca gaaattaatg cactcagatt
    10441 actattcttc ttaccatccg ctcctgaaga aggattcagc aaatacagac caataggaat
    10501 agtaattgcg ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc
    10561 accataaata ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacctgcaat
    10621 taatgcacat acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccgaacca
    10681 ggagaaaaag aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc
    10741 aaaaatcatt tccctgtttg cccgagtaat agatacaaat agagccgcag gaagcgcgta
    10801 gtttaatacc agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc
    10861 gctaaaataa cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt
    10921 tagcataata gtatttccga taagggtccc ctcaccatcg cataatgagt tggcaagagg
    10981 agacgatgtc agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc
    11041 actttcagtt cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg
    11101 aataccggtg gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc
    11161 acgaaacgca tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggagatgg
    11221 tgcaccagcg ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat
    11281 aacgatcgtc accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc
    11341 aaaagcacta tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat
    11401 ggcatagccc ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg
    11461 tttatacata tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa
    11521 ataaatatcc tggttctcgc gcaatacgtc gcgcacagaa cttaatgcat taaagtaatt
    11581 taatggttga gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg
    11641 gatatttaaa atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc
    11701 cagcatacct tgcatactgg atgcaatatc accaatgact ggcacagcaa tggggcggtt
    11761 gctgtcaatt tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc
    11821 ttttttaccg tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc
    11881 atttgccagg gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat
    11941 ccctttcgcc atagacattg gcaggaatgg aatctgagca ctttcaataa attcacgaag
    12001 ctgttcatca gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc
    12061 ttttgctaaa agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga
    12121 cggattttca actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag
    12181 gacatttgct ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat
    12241 agcgcgtgcc aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc
    12301 atacggtttt gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac
    12361 gatcgcgcgg tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt
    12421 ggccaatgcc gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg
    12481 tttttgggta agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa
    12541 accaatataa cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc
    12601 tacaacacca taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat
    12661 accatctgtc atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg
    12721 ggagccagaa gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca
    12781 tcgtcgctat aacctagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca
    12841 gctttaatat ccggcgtaaa agcagagaat ttcattggac aaccaacggt cagatatttt
    12901 ccacgcaacg gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt
    12961 gaaatttctt tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc
    13021 accgcttcat gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa
    13081 atatgtggct gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg
    13141 ccgatggctt tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg
    13201 ttcggatcgg tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct
    13261 gcattaccac cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct
    13321 tccagataac ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc
    13381 aatacggcat cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc
    13441 agcaaagcag caagcaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct
    13501 gcagcgctta ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct
    13561 gcctgagcaa cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct
    13621 ttgatcgaac caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc
    13681 atgtgatcaa tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc
    13741 ttttccatta cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta
    13801 ctgttaagca tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc
    13861 gttacgtcac caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc
    13921 atttgagtac aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga
    13981 agtggagttg acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat
    14041 ttaaggcgta atatcttcta taaataaccc gcaaaatatc gtcgtagctg cactcgatat
    14101 ttttaaaatc ctgtatcaag tgctgaaaat catttcatcg ccacttccaa ttcctttctg
    14161 catggtaagt agtgaggacg aaaagaagaa tatgataaat attgattcgt ggtaatcaga
    14221 gagagtcgcg acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa
    14281 gtaagggtat gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg
    14341 tgatgacacg ggattattgg ggtttacttt gaggaagata aaatgcgtgt ttatagaacc
    14401 gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg
    14461 gctggaggga gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca
    14521 gtgcttcgct gtcgacgatg attccatctt ccgcaccttt cagcgccagg tttgcctcgt
    14581 agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa
    14641 gcggcatcag atattggttt tccattacgc caacaccggc caggcgaagt tcttccattg
    14701 cgcccttttt atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt
    14761 cgggagttgc tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt
    14821 catcattgag attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat
    14881 ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa
    14941 acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac
    15001 gctctgctgc tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta
    15061 ccggtgcatt atcagcagcc catacagttg aagatgccag aattgctgtg accatcgtag
    15121 ccataattaa acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc
    15181 atattgggtg agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct
    15241 tacagttgta gttattatcg caagaagtat tttagtctta caattgtagg aatctgcttc
    15301 ctgtcgcaga gaactattct tatgtaccac aaagagatgt catttattat atcgatgtaa
    15361 atattgatta attatatgta aatacagcca taacatcgta ggttctcagt gaaccatttt
    15421 caggataact ttctatgaaa gtaaacttaa tacttttcag cttattttta ttggcctata
    15481 ttatggcatg caatgttttt gcattttcca tttctggtgg tgtaagtgag aggagctata
    15541 aagagactga aaaaacatca gcgatgacga ccacacactc tacaaaactt cagccatcac
    15601 aggcgatttt gtttaagatg agagaagatg cgccaccatt aaacctcaca gaagaaatca
    15661 cgcctactta tccgacaaag gcgaattatc ttattcatcc tgtgcgatag cgtctgtcac
    15721 atggcaggtg aacaatcatg tctgatgctg atgtgaacac ctgcccaaat gagccatcct
    15781 gacccactat cgcctgccat accccaccat aaagcgaatc atcaagccac aagccggggc
    15841 aagaaacata aatatctccg tcactagttc attaccgcca gttaaccaca tggtgaagta
    15901 ataaatgagt gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt
    15961 cagtacctga ccgatgtagc cgcttacgca gacaatacac agggcgagaa acatccataa
    16021 ataaatcata gctcaacctc ctgccgcgct aaaaaacctg taacttcagt cactttaata
    16081 gtaagacaat ccttaacctc tggcaatttt tgctaagcat caatgaaaac agcatgttaa
    16141 atgcaagact gttgtgtacg gaaaaatatt tactttgcac gattaataat cagtagctga
    16201 aagcagtcag cgagtgataa agtacagata ccgcattcat gctcctcgtg gttatgtcct
    16261 gactagtctt tacacacttt acaggaacca ttgtcgtaca tgatggccca accaattgag
    16321 tgtaccgctg tcgacatgga atcttctgcg atacaacaat tcgtatctac agaaggtaac
    16381 tatgtttcca caatgcaaat tttcccgcga gtttctacat cctcgctact ggctcacatg
    16441 gtttgggctt ggtgtactct ggctttgggt acagcttcct tatcctgttc tctgctttct
    16501 cggcacgcgt attggcgcaa tggcgcgacc attcctgaaa cgtcgtgaat ctatcgcccg
    16561 taaaaacctg gaactttgtt tcccgcagca ttctgcggaa gaacgcgaga agatgattgc
    16621 cgaaaacttt cgttcactcg gcatggcgct ggtagaaacc ggcatggcat ggttctggcc
    16681 cgacagtcgc gtacgtaaat ggtttgatgt tgaagggttg gataacctta aacgcgcaca
    16741 aatgcaaaat cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg
    16801 ccgcgtgatg ggactgtgcc aaccaatgat ggctacctat cgtccacata ataatcagct
    16861 gatggaatgg gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa
    16921 taatctgcgc ggcattgtcg gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga
    16981 tcaggattat ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc
    17041 cacaaccaat ggcacctatg ttctctcccg tctctctggc gcagccatgt tgaccgtaac
    17101 gatggtaaga aaagcggatt acagcggata tcgtttgttc atcaccccag agatggaagg
    17161 ctacccgaca gatgaaaatc aagccgctgc ctatatgaac aagattatcg agaaagagat
    17221 catgcgcgca ccggagcagt acctctggat ccactgtcgc tttaaaacgc gcccggtggg
    17281 agaatcgtcg ttgtacattt aacaaactgc tcttactctt tccctcctca aatcggccat
    17341 aataacctca tggtttttat ggcttttttc ttaaggtttt ttccttcgct gaatatctaa
    17401 ttattaccgt cagaaattaa actgtcgccg atatgagaca cttgtaagtt actaaaagta
    17461 aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc
    17521 agtttagctc agaattctgg ggtgatgccc acggtaaaat acagggcttt taaggtcgct
    17581 ttgcccagtg tcaactcact gtatcaggtg taatgaagtc attcaggcgt aacagcaatt
    17641 acgcggagag atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt
    17701 gggccggtac ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca
    17761 tcaggctcct gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt gaatatgttt
    17821 gctgctggcg ggtagtaaac cgtcggcacg gaacatcgct ttaatccctc tgatggcctg
    17881 acgaatacgg tcgcggtttt caatcagtgc aaagcgaaca tgcgtatcac catagtcgcc
    17941 aaagccaatc cccggcgaga cacagacctt cgcttcgtta agcaatttct tggcaaatac
    18001 cagcgagccc atggcagcat acggttccgg tattttcgcc cagacataca tcgaagcctt
    18061 cggcatttca actatccagc ccgcttcatg cagcccttta accaatacat cacggcggcg
    18121 tttgtactgt tcggcaatgt cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc
    18181 cgccacctgc aacggcgtaa aggtgccgta atcgtgatag cttttaatac gagccagggc
    18241 gctgaccagc gttttgttgc caaccataaa gccgatacgc cagcccgcca tgttgtagct
    18301 tttcgacagc gtaaagaact cgactgccac atcgcgtgca cccggtacct gcatgattga
    18361 cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcgtgga ccaccagcac
    18421 atcgtagcgt ttcgccagcg ccacaacctt ttcgaagaac tccagctcca cgcattgcgc
    18481 ggttggattc gacgggaagc cgaggatcat catcttcggt ttcggataac tttcacgaat
    18541 ggcgcgctcc agttcgttga agaaatcgac accttccacc agcggcactg agcgtacctg
    18601 cgccccggca atcaccgcgc cataaatatg gatcgggtaa cttggattcg gcaccagcac
    18661 cgtgtcacca tgatccagcg tcgccagcat cagatgcgcc aggccctctt tcgaaccaat
    18721 agtgacgatg gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct gataccagcg
    18781 ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat gcgtgtcctg
    18841 gcgctgggcc acagtacata atttttcgac gatatgcggc ggagtcgcac cgtccgggtt
    18901 acccatgctg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc
    18961 ggtgatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg
    19021 agtgtcagcc ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc
    19081 cacaatggtg gctgttttga aaatagcctg attaatttct gtctgtccag cgttttagaa
    19141 catttttatc aatggcttta aatgaggaac tccacttcaa aaacaggaaa tttttctacg
    19201 ctaacaagaa aattaccacc aattttatta acattataat ttcaataaat ttaccagcca
    19261 gaagtgcatt atcaagagat acattgctaa tacaccattt atctttcaat ccccttttaa
    19321 atttatggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt acctatcata
    19381 gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc tgctggcggt
    19441 cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta tccgcctgtt
    19501 tcgcgagctg ttgcacaagt cggcacactc cccaaaagag ttgcttgctg ttaccgccat
    19561 tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag gctcgctggt
    19621 gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt gggtaggcat
    19681 cattaccggc gtgattgcgg gtattcaccg gtatttaatt gatatcggcg gcgtgacggc
    19741 gatcccctgc tttatcacca gcattctggc gggttgtata tcaggctgga taaacctgaa
    19801 aatccccaaa gcacagcgct ggcgcgtcgg tattctcggc ggcatgttgt gtgagacgtt
    19861 gaccatgatt ctggtaattg tctgggcacc aactaccgcg ttggggatcg atatcgtctc
    19921 taaaatcggc attccaatga tcctcggtag cgtctgtatc ggctttattg tgcttctggt
    19981 gcaaagcgtt gagggcgaaa aagaggccag cgccgcgcgg caggccaagc tggcgctgga
    20041 tatcgccaac aaaacgctac cgctgtttcg ccatgtcaat agcgagtcat tacgcaaggt
    20101 ctgcgaaatt atccgcgatg acattcacgc cgatgcggtg gcgattacta ataccgatca
    20161 tgtgctggcc tatgttggcg tgggtgaaca taactatcag aatggcgatg acttcattag
    20221 cccgactacc cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca atgatgaagc
    20281 ccaccgcaca ccagagattc attccatgct ggtgatccca ttgtgggaga aaggcgttgt
    20341 gaccggaacg ctgaaaattt actactgcca cgcgcatcag ataacctcgt cattacagga
    20401 aatggcggtt ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac gcgccgagca
    20461 gctacgtgaa atggcaaata aggcagagct tcgcgccctg caaagcaaaa ttaatcccca
    20521 ttttctgttt aacgctctga acgctatttc atcgtcaatc cgtctgaatc cggataccgc
    20581 tcgccagttg atctttaatc tgtcgcgtta tctgcgctat aacattgaat taaaagacga
    20641 tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa gactatattg ccattgagca
    20701 ggcccgcttt ggtgacaagc tgacggttat ctatgatatt gatgaagagg tgaattgctg
    20761 cattcccagc ctgctgatcc agccgttggt ggagaacgcc attgtccacg gtattcagcc
    20821 ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag tgcggaaatc gggtacgcat
    20881 tgcggtgcga gataccggca cggcatcgat ccaaaggtga ttgagcgggt cgaagcgaat
    20941 gaaatgccgg gcaataaaat tggcctgctg aatgtccatc atcgcgtgaa gttattgtat
    21001 ggcgaggggc tgcatatccg ccgcctggag ccggggacgg aaattgcgtt ttacattcct
    21061 aaccaacgca ccccagtcgc ctcacaggct acgttattgc tttgagccgg agtgatattg
    21121 tgaaagtcat cattgttgaa gacgaattcc tggcacaaca ggaactgagc tggctaatta
    21181 aagagcacag ccagatggag attgtcggca cctttgacga cggtctggac gtgttgaagt
    21241 ttttgcagca taaccgcgtc gacgccattt ttctggatat caatattccg tcgctggatg
    21301 gcgtgttgct ggcgcaaaac atcagccagt tcgcccataa accgtttatt gtgttcatca
    21361 ccgcgtggaa agaacatgcg gtagaagcgt ttgaactgga ggcgtttgac tacattctca
    21421 aaccgtatca ggagtcacgt attaccggga tgctgcaaaa actggaagcg gcctggcaac
    21481 aacagcagac cagcagtacg cctgccgcga cggtaacgcg tgagaatgac accattaatc
    21541 tggtgaaaga tgagcgaata atcgtcacgc caattaacga tatctattac gccgaagcgc
    21601 acgagaaaat gacctttgtc tatacgcggc gtgaatccta cgtaatgccg atgaacatta
    21661 ccgaattttg cagcaaactg ccgccgtcgc attttttccg ctgccatcgc tcattttgtg
    21721 tcaatctgaa caaaatacgc gaaatcgaac cgtggtttaa taacacctac attctgcgac
    21781 tgaaagatct ggattttgaa gtgccggtca gccgcagcaa agtgaaagaa tttcgccagt
    21841 taatgcatct ttaatcggga actttcatga aagcaccagg cttgcccgcc gatcaacaat
    21901 ttttcgccga tctgttcagc ggcctggtgc ttaactcgca actactgggg cgcgtctggt
    21961 ttgccagcca gcctgcctcg ttaccggtgg gcagtttatg tattgatttt ccccgtctgg
    22021 atatcgtgct gcgcggcgaa tacggcaacc tgctggaagc aaagcagcag cgaatggtgg
    22081 aaggagaaat gctgtttatt ccggcacgcg cggctaattt accgatcaac aacaaaccgg
    22141 tgatgctgtt aagcctggtg ttcgcaccga cctggcttgg gttatcgttt tacgatagcc
    22201 gcaccacgtc gttgttgcat cctgctcgcc agatccagct tcccagcctg caacgcggtg
    22261 aaggtgaagc gatgcttacc gccctcactc atcttagccg ttcgccgctg gagcagaata
    22321 tcattcagcc actggtgtta agtttgctgc atctttgccg caacgtggtg aatatgccgc
    22381 cgggtaattc gcaaccgcgc ggcgattttc tctatcacag catttgtaac tgggttcagg
    22441 ataattatac ccagccgctc acccgcgaga gcgtggcgca gttttttaat atcacgccca
    22501 atcatctgtc aaaactgttt gctcagcatg gaacgatgcg ttttatcgag tatgtgcgtt
    22561 gggtgcgaat ggcgaaggcg aggatgattt tgcagaaata tcatctgtca attcatgaag
    22621 tggcacagcg ttgcggtttt ccggatagcg actatttttg tcgcgttttc cggcgtcagt
    22681 ttggtctgac gccgggagag tacagcgccc gttttcaggg ctaacgtcag aaggttaatt
    22741 ctgtttccag cagcgtcagg atactttgtg catcttgcgc ggcaaacaac gactggcgga
    22801 agtttttatt caccagttta cgcgccagct gcgagaagac tttcacatga ttcatccctt
    22861 cgttagcacc cagcgtcagc atgatcacca gttcgacttc gcccatttct gactgccagt
    22921 caatcggttt cgccagccgg gcaatgctga tactggaatg acggatccac tgagatttgg
    22981 tgtgcgggat cgctacgcca aaaccaacgc cggtggtaac aatctcttcc cgctgccaga
    23041 catcttcttc cagctcaaac ggatgttcag tgcgcccgtt aacgccgagg ttgccgcaca
    23101 ggaactggat cgcctgctct ttattgctaa aatcctgatc aacaaagata ttctccagcg
    23161 ccagcagtgg gcgaacgtct tcttccggcg taaaggcggt gagtaacgct tcaatttcct
    23221 gcgcactgcg gcattcacat gcctgacgcg ccagttcccg acacgcctcg ctatccagtt
    23281 gacgaagctg gcttttcacc gccggaatac gcgggctact catactcagc tcatccaggc
    23341 ccagcccaag cagtagcggc agataacggc tttcaccgcc cagttcaccg caaatgccta
    23401 cccatttgcc ccgctgatgc gcagtggtaa ctatttgctg caacatgcgc aggaacgatg
    23461 gcgtaatcgg gttatatagc ggcgatacgc gcgggttatt acgatcgacc gcatacagat
    23521 actgggtcat atcgttggag ccgatactga agaaatcgac ctcatcgcag aagtggtcga
    23581 tgatgtagca caccgacggc acttccacca tgatcccaag cgtaatcgtc tctgcatgac
    23641 gcaggccatc gcgcttaagc tcaacgatcg ctttttggat ctcgcccttc acccataaga
    23701 tctgatcgag actgtggacc atcgggatca tcaactgggc gttgccaaaa ctggcggcgc
    23761 gcaaaatcgc ccgtagttga gtgcagaaca ggccagcaaa ttccgggtaa atacgtaccg
    23821 cgcgatagcc gaggaacggg ttctcttcct ggggaatatt cagataagga atgcttttat
    23881 cgccaccgat gtccattgtg cggaagataa tcggcttgtc gcccgctgcc agtagcacct
    23941 gctggtaggc ttcaaattgc tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca
    24001 tttcagtacg gaacagtcca acgccttccg cgccgttggc aaacgcgcct ggcgcttcca
    24061 gagcggtgcc gatattcgcc gcaatatcaa tacgcttgtt atcacgggaa taggccagct
    24121 gcgcggcggc ctgcgcctgt tgtttttggc gtttatccgc cagcgtctgt gcgacctgat
    24181 aataaccgct caccgcgtca ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca
    24241 ccgccggttg cccggcataa cgggcaatcg catccagcgg caggccgctc agtaccggga
    24301 tcgccgaggc gcgggccaga atcagggtat gcgaggtgcg cccagttttc tccagaatca
    24361 tgcctgcaag attttttaaa tcgaggctca aaaactggct tggggttaaa tcctcggcta
    24421 ccagaatagt cggtttttcc agcaccagat tgttgcgcgg cttcagttcc ggccaggtga
    24481 tatgcagcaa ctgttcgcta atatcgcgaa tgtcgctaac acgttcgcgc agataatcac
    24541 tggcagaagc agaaagtttg gcgcaaacct gctccatatt gctgatgatc gccgccccca
    24601 gcccttgatg ctgttctgtc atcagacgac ggatattgcc cgcgaattca tcatcctgaa
    24661 tcagcgacaa atgggcgcta aggatagttt tgctttcgcc gtcactctca cgcaattgct
    24721 ggttcagttg ctcggcaagc gttgcaaggc tgtgctccag ccgggtggaa tcttgcgcac
    24781 tggcggggat tgcccgataa ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca
    24841 cgccgacgct gcttgccagc acattgccgt acagtaaatc cgggttcagg cggcttaatg
    24901 aacgcggcag cggatgcgcc gtcagttctg cctgcgttgg ctggatgctg tcgctgtcga
    24961 taaagcgcac ctggatgtac tcttccaaca cgcgctgcgc ctgctcttca tcgctgccgc
    25021 taatgttcag gctgcaactg tcattaaata aagtaccggt gccaatcagc gccagcgagc
    25081 ttttcgcatc ggcctttgcg ttctggcgat ggttgataaa ggtgatttcg ctttgccact
    25141 ggctgcactg ttctttaagt tcccacgccg gacgagcatg aagaccgttt ggcaaaggac
    25201 agagaaattg aatcgttaac ataaccgctc cgggttaatc aggagatcat ctgaaatccg
    25261 tcagttgaac aaccgtctca cgcgtaagac gtttaataag ggcagataaa agctgctgca
    25321 tctgcaaaat gtcacggcaa tcggcaatcg atgcggcgca atgtccgtgg cgggttgctg
    25381 gccccatcac cactgtaggc acgccggtgc cggttaagtg caccgctccg ccgtccgtgc
    25441 cgccgttgct gaacatatct gcctgtaacg gcacgccaat ttctgccgcc acggtttcga
    25501 tccacgcggt aagttttggc ggcgcaatca gcgacttgtc gcttaacacc agcatcggcc
    25561 cgttacaaat ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc cagcaggcgg
    25621 tatcaagcac aatggcgaca tccggcgaca ccgcgcgggt ggcagtttgc ccgccgcgta
    25681 cccccacctc ttcgctggaa ctggccacca gccacacttc cgcaggcagt tcagcgctgt
    25741 gtagttcgcg cagtaacgtc accagcaggt agcaaccgag gcggtcatca aaggctttcc
    25801 ccatcacccg ctggtgaggg agaacctgaa aagttgtatc aaaggtgacg cgatcgcctg
    25861 gacgaattcc cgcctgcatc acttcgtcat acgagcgcgt accaatgtcc acgcgcatgg
    25921 cgctgacgtc attcccctgc cggtcgccgt caagcaggcc tggaattttg cactcttcac
    25981 gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgcacgttg ccaaccggca
    26041 gcacatcaat tgccccttcg cgggagatgc tgcgcaccat aaatcccact tcgtccatat
    26101 gggcgcagat catcaccttc ggccctgtcg attcattgag gcggatcagc accgatccca
    26161 gaccatcaaa tcgcacttct ttttgcaggc gatccgcttc ttccagcagg atctgccgca
    26221 cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat agcgataaat
    26281 ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca cttctgcgcc
    26341 ttgcggggta accagcacaa catcttcgat gcgcacgccc ccttgccctg gcaaataaat
    26401 ccccggctcc acggtcagca acatgcctgg ctgtagcgtc gtggtgtccc gcggtgaaaa
    26461 acgcggatct tcatggactt caatgccgat agcgtgacca gtgttatgac cgaaatagtc
    26521 gccaaatcca gcctcggtaa tgacccggcg cgcggcgtcg tcaatctgct ggcagcgcac
    26581 gccggggcga attgcagaga ttgccgcgag ctgtgcctgc aggacaatct gatagacgtc
    26641 aaacagcggg tgagattcgg cgctcacccc ttcgccattc accagcaagg tgcgcgtcat
    26701 atcagagcag tagccctgat acagcgcacc aaaatcgaga gtgacaaact cgcccgctgc
    26761 aacaatcttg tcgctggctt tgccgtgcgg cagcaccccg cgccagccgc tggcgacaat
    26821 ggtgtcaaaa gaggcttttt ctgcgccctg ctggcgcata aaccactcca gttcagcggc
    26881 tatctcgcgc tcgctcatcc ccgcctgaat aaagcggcga atatgctccg ctccgcgatc
    26941 ggcaatccca caggcgaggc ggattatctc cacctcctct ggcgttttga tttgccgcag
    27001 cacatccggc gtggcgctaa ccagtttcgc attgagttca gacttccagc ggtgcgcggt
    27061 ttcccaactc acctgctggc cttcaaaacc gagcgtttgc aactgctcat cggcaatgat
    27121 ttgattgacg atagtggtaa gcgtgttcgt cgcgtcaagc aaatgcagct ggtagccttg
    27181 cgcacgggct tccacctcaa cgaaatagct cgaatctacc agaatgtgcg cactttcacg
    27241 gctaataacc acatagcctg atccggtgga gatccccaga tgcggctgtt tgttctgccg
    27301 tgaggagaga agcactgcat ccagttgttg ctccttaagc cagtcgcgca gcgaagcgag
    27361 taatgtcatc tggattttcc ttcttacagg ctatcgatta acaatttgcc tttacggaac
    27421 atcatcagac gcaggaacac caccatcagc gcagttatga ctgcccccag cgcgatcccc
    27481 gccatataga cgccaaggtt agtgaccagc ggccatgccc agatagcgga ttccgggaac
    27541 cattgcactg cgcccagcca gacagcggcg gttgagccga caatcgcgcc gaccatatac
    27601 gacggaatgg cggtgatggg gctttccagc gcaaacggaa tcgcaccctc actaatcccc
    27661 ataaaggcaa ggaacatcgc ggtcttaccc tgcggataaa gttgagcgtt gaacaggcgt
    27721 ttgccggtta aacgacggtc aataatggtc gccagcccca gaccaatcgg aggaataacg
    27781 atagcgatag aacgagcggt gaccggtaat acgtggtcag tggtaaagct gaaggcaacg
    27841 aaacctgcgg ctttgttgat cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc
    27901 attgcgtaca tcagcgcgcc cttctcacct gcggcagtca gcacggtacg gataacgccg
    27961 ttgatccagc cgccaaaggg ggtaatgacg tagtacatcg ccagcatgac aaaaatggcg
    28021 gaaagaatcg gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg taactggatt
    28081 ttctggttca tccacttcac aaggtaaccc gcgacgatag aaataatcag cgcaccaatg
    28141 aaggtggacg gcaccggcga agaggtcgcc cattgcatag tgctgggatc gaagttcagc
    28201 agttgcgtcg gctgggtaga catcaagccg ccgataaaac cagccgggaa cgccagtttg
    28261 ccaccgatag agttcgccac aaaagcagca aacatcggaa tggcaaaacc aaacaacacg
    28321 ccgccgaagg actgcgacag ccaggcaaat ttcagtaagg agaggtcgaa accggagaat
    28381 ttcccgctat taagagcatc catgatgccg atctcagcag gaattttaag ccagctataa
    28441 gcaatcagct ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt
    28501 gaaatccctg acatcacatg ctgcgggagt tcaccccaga agctgctttt agaggcctgc
    28561 ggattcttaa ctgccgctgc cgcaccggat gcgccaggca caacggttgc actgcgtttt
    28621 ttaatggcca taacaattat ccctgtcgaa tattactgtt gttcagaggc aatcatctct
    28681 tcgatttctt tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa
    28741 acgtcgcgtg attcgaaacg ctcattatct tccggggtaa ctgccacgga gtggatgatg
    28801 atggttgctt cggcgatatc ctgcgccgtc aggcgattct ggataccgtc cgcgccctgg
    28861 gtttcaattt tcacttcata accggcttct accgccgctt cttccaacgc ctgagcggcc
    28921 ataaaggtgt gagccaggcc cattgggcag gcacataagg caatcagttt cttactcatc
    28981 gttaattcct cattcagtga tagttgacaa gtgaaattat gaacgtgcaa aacaaatcgc
    29041 cgttaccaga caaataatgc atttactgga gaattaatcc cgctgtacgg aagtgtgacg
    29101 caggtcggcc ttgtgagggg acacaaagaa aaattgaatt aactgtatga tttaaacgat
    29161 tatcgggaga gttacctccc gatataacag gaaggattta cagaatgtgc cctaaggtct
    29221 ggcgtaaatg tgcaccggaa ccgagaagac ccggattgtc atggacgatg agatacaccg
    29281 gaatatcatg gacatattct ttaaagcgcc ctttatcttc aaatgcagca cggaaaccag
    29341 aggctttgaa gaactcaagg aagcgcggca cgataccgcc cgcaataaac acgccgccaa
    29401 atgtcccgag attgagcgcc agattgccgc caaaacggcc cataatgacg caaaacagcg
    29461 acaacgcgcg gcggcaatcg gtacagctgt cagccagcgc gcgttcggta atatcttttg
    29521 gcttgagatt ttctggcagg cggttgtcag ctttcacaat tgcgcgatac aaattcacca
    29581 gcccagggcc agaaagcacg cgctccgccg aaacatgacc aatttccgca cgcaatattt
    29641 cgaggataat ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg
    29701 gcaagcttac ccaacgctta tcgacatgga ccagatgcgc aaccccaagc cctgttccgg
    29761 caccgtaaac cgcaataggt ttaccttcta ccggttctgc gccaccaaac tgaatcagat
    29821 gctctttttt cagcatcggg atcgccatcg atacagcggt aaaatcgtta ataatttcca
    29881 gatggctaaa accgagattc tttttcattt cggcaattga gaacgcccag gtatggttgg
    29941 tcattgccac ccagtcaccg gtaattgggc aagcgatggc aatacagccg tctttcacct
    30001 cgaccttatg ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc
    30061 ctgaataggt cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg
    30121 cgttggtgcc gcccacatca ccgactaatg catactttat cattcttcaa ctgctccgct
    30181 aaagtcaaaa taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga
    30241 aaggcaggct ccctgtaaat atcgatctgg gtcacacatt tactttatcg tttcagcacc
    30301 aattgcagcg atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa
    30361 tagaacatgc tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca
    30421 attgggattg cgtccagtct tattctgatt gtggtgatga aaatcgcctc ggtattacag
    30481 aatttgctct ggcaacgact gccgggaact ctggggatag cccaggattc acctctctgg
    30541 atcatcggtg tattaacgct aacgggtatt gcggtggggt tggttatccg tttcagccag
    30601 ggtcatgccg gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccagt tccgccctct
    30661 gcgctaccag gacttatcgt agcattaatt ctcggtcttg ctggcggcgt cagcctgggg
    30721 ccggaacatc cgatcatgac catcaatatc gcccttgccg tcgccattgg cgctcgtctg
    30781 ttaccgcgcg tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg
    30841 ctgtttggca cacctgttgc agcggcgttg atattttcgc aaaccttaaa tggcagtagt
    30901 gaagttccgc tatgggatcg tctctttgcg ccgttaatgg cggcagcagc tggtgcacta
    30961 actaccggat tatttttcca tccccatttt tcactgccca ttgcgcatta cggacagatg
    31021 gaaatgaccg atattctcag cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg
    31081 gtcgccgtat ggtgcttacc acggttgcac gcgatgatga atcaaatgaa aaatccggtg
    31141 ctcgtgctgg gtattggcgg atttattctc ggtattctgg gggttattgg tggaccagtt
    31201 tcgctgttta aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc
    31261 agcgattact ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt
    31321 ggctttcgcg gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg
    31381 ctgcatgagc acgtacccgc cgtaccagcg gcaataaccg tttcttgcgc tattctcggc
    31441 atcgtgctgg tggtaacacg cgatggctgg ttaagtcttt ttatggcggc ggtcgttgta
    31501 cccaatacca cattgctacc gctgctctgt atcgtcatgc ttccggcatg gctgttatta
    31561 gcaggtaagc cgatgatgat ggtcaatcgt ccgaagcaac agccacccca cgataacgtt
    31621 tagcaaaaaa tgctctcctt tatgatttaa gagttatggc gatgatacgg ggctttacct
    31681 ccccgtaata ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg
    31741 ttcaggtcac tgtttctggc ggccgccctg atggcattta ccccgcttgc agcaaacgca
    31801 ggtgaaatca ccctactgcc atcaatcaaa ttacaaattg gcgatcgcga tcattacggt
    31861 aattactggg acggtggtca ctggcgcgac cgtgactact ggcatcgcaa ttatgagtgg
    31921 cgcaaaaacc gttggtggcg tcatgataat ggctatcatc gtggctggga taagcgtaaa
    31981 gcgtatgagc gtagctatcg tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc
    32041 cgcggacatg gacaccgcca ttaaacacat tcgccaatgg agcacaatgc ctgatgcgac
    32101 gctagcgcgt cttatcaggc ctacaaaccg gatctttaac gcgtagggcg gataaggcgt
    32161 ttacgccgca tccgccaata gtgccagatg cgacgctcat tcaactacaa tcccagcgcc
    32221 gtccccacca acaaccagat attcagcgcc acgaccagca ctacaatcac ccagcctgtc
    32281 tgttttacgc gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc
    32341 agcagtggaa ccagcgccag ggcgatacca aaacttaaca gcacctgact cataaccaga
    32401 atccgtgtcg gatctaatcc catcagaatg acaataaatg acggcagcat ggtgactgta
    32461 cgacgcaccc acagcgggat atggaagcga atgaacccct gcatcaccac ctgccctgcc
    32521 agtgtcccca ctaccgttga ggacagtccg gcagcaacca gacttaaccc aaagaccgtt
    32581 gccgcagcat ggcttaacag cggttgcagc gtcagataag cctcatcaag atcggcaaca
    32641 ccagtatgac cagaaaagtg gaacgccgcc gcagctgtag ccatcatcgc cagattgaca
    32701 aaaccggcaa tagtcatggc gatagccaca tcccatttgg tggcggaata acgttgttga
    32761 cgcgaaccgc catgtaaatg ctgagtgagc gaggagtgca aataaatcac atgcggcata
    32821 atcgtcgccc ctaacacgcc tgctgccagg aagaccgctt ccgaagtagg taaactcggg
    32881 agcaccattc ctttacccag ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg
    32941 taagccgcgg caacaaacaa cagtaacccg ccaatcactt tctccagcgt tttttgcccg
    33001 cgacgttgca gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac
    33061 aacgaaacac caagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg
    33121 gttgccatcg caataatttc tgcctgaacc caatagaacc acactacggg acgcggatag
    33181 tgatcgcgaa tctgctccgc cagattttta ccggtggcaa tccctagttt ggcagagagg
    33241 atctgaatca gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg
    33301 aagctggcac ccgcctgaat attggtcgca aagttaccgg gatcgatata accaatcgcc
    33361 gcaatgaacg caggtcccat taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta
    33421 ctctcaacgc gatagttcgt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat
    33481 gctatgataa acgagaatga ttatcaaatt catttaaatg gattgtggtg atttctgtga
    33541 tagaccagga ttatgactac gaaaagattg atgacttgaa tgttatggtt atgtttaatg
    33601 ttagcacatt tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagat
    33661 cactattttt gaagcctgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct
    33721 taaccatgtt acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat
    33781 ggaccgcgtc cttcattttg tactggcact tgccgttgtt gcgattctcg cactgctggt
    33841 aagcagcgac cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt
    33901 gttactggcg tggttcttcc tgaactccga cgttggttta ggcttcgtga aaggcttctc
    33961 cgaaatgttc gaaaaactgc tcggatttgc caacgaaggg actaacttcg tctttggtag
    34021 catgaatgat caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat
    34081 ctctgcactg atcggtattc tccagcacat tcgcgtgttg ccggtgatca tccgcgcaat
    34141 tggtttcctg ctctccaaag tcaacggcat gggcaaactg gaatccttta acgccgtcag
    34201 ctccctgatt ctgggtcagt ctgaaaactt tattgcctat aaagatatcc tcggcaaaat
    34261 ctcccgtaat cgtatgtaca ccatggctgc cacggcaatg tccaccgtgt cgatgtccat
    34321 cgttggtgca tacatgacca tgctggaacc gaaatacgtc gttgctgcgc tggtattgaa
    34381 catgttcagc acctttatcg tgctgtcgct gatcaaccct taccgtgttg atgccagtga
    34441 agaaaacatt cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga
    34501 atacattctg gcaggtttca aagttgccat tatcgttgcc gcgatgctga tcggctttat
    34561 cgccctgatc gctgcgctga acgcactgtt tgccaccgtg actggctggt ttggctacag
    34621 catctccttc cagggcattc tgggctacat cttctatccg attgcatggg tgatgggtgt
    34681 tccttccagt gaagcactgc aagtgggcag tatcatggcg accaaactgg tttccaacga
    34741 gttcgttgcg atgatggatc tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg
    34801 catcatctct gtgttcctgg tttccttcgc taacttctct tcaatcggga ttatcgcagg
    34861 tgcagttaaa ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaaact
    34921 ggtttacggc tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta
    34981 agaccataca taaaaaagcc ggggataatt c
//
LOCUS       DAJTOR010000023        34834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTOR010000023 DAJTOR010000000
VERSION     DAJTOR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.023"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(122..1381)
                     /locus_tag="OO249_000987"
     CDS             complement(122..1381)
                     /locus_tag="OO249_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5635363.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(1572..2435)
                     /locus_tag="OO249_000988"
     CDS             complement(1572..2435)
                     /locus_tag="OO249_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5635364.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            2562..3278
                     /gene="rph"
                     /locus_tag="OO249_000989"
     CDS             2562..3278
                     /gene="rph"
                     /locus_tag="OO249_000989"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5635365.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            3344..3985
                     /gene="pyrE"
                     /locus_tag="OO249_000990"
     CDS             3344..3985
                     /gene="pyrE"
                     /locus_tag="OO249_000990"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5635366.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(4022..4618)
                     /gene="slmA"
                     /locus_tag="OO249_000991"
     CDS             complement(4022..4618)
                     /gene="slmA"
                     /locus_tag="OO249_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5635367.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(4725..5183)
                     /gene="dut"
                     /locus_tag="OO249_000992"
     CDS             complement(4725..5183)
                     /gene="dut"
                     /locus_tag="OO249_000992"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5635368.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(5161..6381)
                     /gene="coaBC"
                     /locus_tag="OO249_000993"
     CDS             complement(5161..6381)
                     /gene="coaBC"
                     /locus_tag="OO249_000993"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5635369.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSENNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            6553..7221
                     /gene="radC"
                     /locus_tag="OO249_000994"
     CDS             6553..7221
                     /gene="radC"
                     /locus_tag="OO249_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5635370.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            7438..7674
                     /gene="rpmB"
                     /locus_tag="OO249_000995"
     CDS             7438..7674
                     /gene="rpmB"
                     /locus_tag="OO249_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5635371.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARSEKY"
     gene            7695..7862
                     /gene="rpmG"
                     /locus_tag="OO249_000996"
     CDS             7695..7862
                     /gene="rpmG"
                     /locus_tag="OO249_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5635372.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            7960..8769
                     /gene="mutM"
                     /locus_tag="OO249_000997"
     CDS             7960..8769
                     /gene="mutM"
                     /locus_tag="OO249_000997"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5635373.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(8808..9287)
                     /gene="coaD"
                     /locus_tag="OO249_000998"
     CDS             complement(8808..9287)
                     /gene="coaD"
                     /locus_tag="OO249_000998"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5635374.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(9295..10572)
                     /gene="waaA"
                     /locus_tag="OO249_000999"
     CDS             complement(9295..10572)
                     /gene="waaA"
                     /locus_tag="OO249_000999"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5635375.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            10985..12043
                     /gene="rfaQ"
                     /locus_tag="OO249_001000"
     CDS             10985..12043
                     /gene="rfaQ"
                     /locus_tag="OO249_001000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5635376.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLRVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            12040..13164
                     /locus_tag="OO249_001001"
     CDS             12040..13164
                     /locus_tag="OO249_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5635377.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            13157..13954
                     /gene="rfaP"
                     /locus_tag="OO249_001002"
     CDS             13157..13954
                     /gene="rfaP"
                     /locus_tag="OO249_001002"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5635378.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            13970..14986
                     /gene="waaO"
                     /locus_tag="OO249_001003"
                     /gene_synonym="rfaI"
     CDS             13970..14986
                     /gene="waaO"
                     /locus_tag="OO249_001003"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5635379.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            15003..15998
                     /locus_tag="OO249_001004"
     CDS             15003..15998
                     /locus_tag="OO249_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR5635380.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            16008..16700
                     /gene="rfaY"
                     /locus_tag="OO249_001005"
     CDS             16008..16700
                     /gene="rfaY"
                     /locus_tag="OO249_001005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5635381.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            16726..17751
                     /locus_tag="OO249_001006"
     CDS             16726..17751
                     /locus_tag="OO249_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCR5635382.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            17836..18819
                     /locus_tag="OO249_001007"
     CDS             17836..18819
                     /locus_tag="OO249_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5635383.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIVNKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRDCNLPGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            18865..20118
                     /locus_tag="OO249_001008"
     CDS             18865..20118
                     /locus_tag="OO249_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5635384.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQYLLDK"
     gene            complement(20188..21159)
                     /gene="rfaC"
                     /locus_tag="OO249_001009"
     CDS             complement(20188..21159)
                     /gene="rfaC"
                     /locus_tag="OO249_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5635385.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(21163..22209)
                     /gene="rfaF"
                     /locus_tag="OO249_001010"
     CDS             complement(21163..22209)
                     /gene="rfaF"
                     /locus_tag="OO249_001010"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5635386.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            complement(22219..23151)
                     /gene="rfaD"
                     /locus_tag="OO249_001011"
     CDS             complement(22219..23151)
                     /gene="rfaD"
                     /locus_tag="OO249_001011"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5635387.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            23365..24561
                     /gene="kbl"
                     /locus_tag="OO249_001012"
     CDS             23365..24561
                     /gene="kbl"
                     /locus_tag="OO249_001012"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5635388.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            24571..25596
                     /gene="tdh"
                     /locus_tag="OO249_001013"
     CDS             24571..25596
                     /gene="tdh"
                     /locus_tag="OO249_001013"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5635389.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            25835..26869
                     /gene="waaH"
                     /locus_tag="OO249_001014"
                     /pseudo
     CDS             25835..26869
                     /gene="waaH"
                     /locus_tag="OO249_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(26856..27815)
                     /locus_tag="OO249_001015"
     CDS             complement(26856..27815)
                     /locus_tag="OO249_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5635390.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(27819..29102)
                     /gene="envC"
                     /locus_tag="OO249_001016"
     CDS             complement(27819..29102)
                     /gene="envC"
                     /locus_tag="OO249_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5635391.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(29112..30656)
                     /gene="gpmM"
                     /locus_tag="OO249_001017"
                     /gene_synonym="pgmI"
     CDS             complement(29112..30656)
                     /gene="gpmM"
                     /locus_tag="OO249_001017"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5635392.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            30901..31332
                     /locus_tag="OO249_001018"
     CDS             30901..31332
                     /locus_tag="OO249_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5635393.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            31474..31725
                     /gene="grxC"
                     /locus_tag="OO249_001019"
     CDS             31474..31725
                     /gene="grxC"
                     /locus_tag="OO249_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5635394.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            31788..32255
                     /gene="secB"
                     /locus_tag="OO249_001020"
     CDS             31788..32255
                     /gene="secB"
                     /locus_tag="OO249_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5635395.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            32255..33274
                     /gene="gpsA"
                     /locus_tag="OO249_001021"
     CDS             32255..33274
                     /gene="gpsA"
                     /locus_tag="OO249_001021"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5635396.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            33354..34175
                     /gene="cysE"
                     /locus_tag="OO249_001022"
     CDS             33354..34175
                     /gene="cysE"
                     /locus_tag="OO249_001022"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5635397.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(34228..34701)
                     /gene="trmL"
                     /locus_tag="OO249_001023"
     CDS             complement(34228..34701)
                     /gene="trmL"
                     /locus_tag="OO249_001023"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5635398.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
BASE COUNT         8710 a         8266 c         8695 g         9163 t
ORIGIN      
        1 aaaggggcgg gtggtaattt tttcattgtg tcacctttta cgctgcgcct tattttgtga
       61 ttgagccagg cggtaaggct tcccgctttt cgccccgtcg agctaggctc aatctattca
      121 gttagttcgc ccttctgaga ggcgttacgt tgtagtcttt tccgctttcc agtgccacca
      181 gcagatcagc ccactgtgcc agtgctgctt tacgctcatc aaaatactga tggcggttat
      241 aaatcccctc tacgccctta atgcgatgat tgaggcatcg ttccgccaca acaggatcaa
      301 cacccaacgc tgctaaatga gtacgtgcgg tgcgtcgaaa gtcgtgaatc gtgaaattag
      361 gcacatccgg catttcggcg cgaaccttag ccagtgctac gggtaaagtg ctttcctgaa
      421 tatgtgggat cattctgttt tgcatcttcc tggccggaag cacccatgcg ctattacatg
      481 aaaaggtgtg tagctctctc aaccattcaa cggctgggga aggtagaggt atatcaatag
      541 ggtctccgtt tttgctgcgt tcttccggca aatgccatac cgcaccatct aaatcaaact
      601 cttcccatcg tgcggcgcat aattccattt tgcggacgca taacgctaac agtaatttga
      661 acgtgatttc gttctggcga ctgaatccct tagccgtgcg catggctttg aatagctgga
      721 ttaactcatc gcgggttaac cagcggtcac gggcagcttc tttccctccg gcgtctgcca
      781 cctcaaaggc tgaacagggg ttaatctcta gcgcgtgccg tttgattccg tagtcgaata
      841 tgcggcgcgt ccatctcagc acgtccgttg ctatggtcgg cgctccacgg tcaacaatac
      901 ctttcagcat gtcatcgata tggcgcggtt tcacgtcctc taccttcatg ctgccaatgc
      961 aggggtttat atctttgtcg atacggcggc ggagtatatc ggggtgcttc cagcgcggga
     1021 ggatctgacg ctcaaagtat tcagcggcaa gctctgaaac gcgcatagcg ttcttctctg
     1081 cttccatctt cgccagtgct tcggttttgc gcttctgctt ctctcctgca acgtcatagc
     1141 ccagcgcaac gcgagccgat agctctttgg ctgtctctct ggcctttgat agtgatagct
     1201 cgctatacga accaattagc atggcgcggg acttccctgc gagtttatag cggaatctcc
     1261 atgtgggggt tttgtcggct tcacggtaac gtaggtataa accgttaccg tctgcccgcc
     1321 cctcgaatcg ctctccgctc ttaatccatg cgcggatctg catgtctgtt agttttggca
     1381 ttttgcgaat atccaaactt tgaatagggg gtgtacccaa agcaatgtac ccaaacctca
     1441 ttgggtacac ctttgtgtac accataatca tgcgctttgt tgcgtcttta tgatagccca
     1501 tgataacaaa aaagcccgct gggttgcggg cttggtgtga tttatgcgtc gtcctaaatg
     1561 ttgctgatag cttactcgat attctgaatc tgctcgcgca tctgctcaat caacactttc
     1621 agctcaatcg cggagtttgt cacttcggca ttgatagact tcgacgcaag agtgttcgac
     1681 tcgcggttga attcctgcat cataaagtcc agacgacgac caacggcttc ttttttcttc
     1741 agaatgttgt aggtctcttt gacatgcgct tccaggcggt ccagttcttc ggcaacgtca
     1801 attcgttgtg ccagcagaac cagctcctgc tccagacggt tgttttccaa ctgaacctga
     1861 gcatcttcca gcttcgcgac cagacgctca cgctgccatt gcaggatttc cggcatatgg
     1921 gcgcggactt tgaccacttc ggcggtgacg ccttccagac gctgctcgat caatgctttc
     1981 agtgcctgac cttcggtttc gcgcgcgaca ataaagtcgt ccagcgtacc atccagcgcc
     2041 gcgagaattt cagcggcaat ggcgtcaaga tcctgctcct gggctgccat cacgcccggc
     2101 cagcgtagaa tatcaaccgg gttgatttcc ccttcgtcac tctgcatttt tacccagttc
     2161 gcggcagtta ccagctgttt agccagtttt tcgttgagga tcagctcacc ttgcgcgcta
     2221 atatctggct catagcgcag ggtacattcc actttaccgc gcgtcaggcg agaacgaata
     2281 cgctcgcgaa cgacaggttc aaggctacgg aactgctccg gcagacgaaa gtaagtttcc
     2341 agataacgct ggtttaccga gcgcatttcc caggttgcgc tcccccattc acccttgatt
     2401 tcacgccggg cgtaggcggt catactgcgg atcatagacg ttcctgttta taaaaggaga
     2461 ggtggaagga ttatagccat cgatgccttg tcaggatagg aataaccgcc ggaagtccgt
     2521 ataatgcgca gccacatttg tttcaagccg gagatttcaa tatgcgtcca gcaggccgta
     2581 gcaataatca ggtgcgtccc gttaccctga ctcgtaacta tacaaaacat gcagaaggct
     2641 cggtgctggt cgaatttggc gataccaaag tgttgtgtac cgcctctatt gaagaaggcg
     2701 tgccgcgctt cctgaaaggt cagggccagg gctggatcac cgcagagtac ggcatgctgc
     2761 cacgttctac ccacacccgt aacgctcgtg aagcggcgaa aggtaagcag ggtggacgca
     2821 caatggaaat ccagcgtctg atcgcccgtg ctcttcgcgc ggcagtagat ttgaaagcgc
     2881 tgggtgagtt caccattacg ctggactgcg acgtgcttca ggctgatggt ggcacgcgta
     2941 ccgcgtcgat tacgggtgcc tgcgtggcgc tggcagatgc gctacagaag ctggtggaaa
     3001 acggcaagct gaaaaccaac ccgatgaaag ggatggtagc cgcagtttct gtcggaattg
     3061 tgaacggcga agcggtttgc gatctggaat acgttgaaga ctctgccgca gagaccgaca
     3121 tgaacgtagt gatgaccgaa gacgggcgca tcattgaagt gcaggggacg gcagaaggcg
     3181 agccgttcac ccatgaagag ctactcacct tgttggctct ggcccgaggg ggaatcgaat
     3241 ccattgtagc gacgcagaag gcggcgctgg caaactgatt tttaaggcga ctgatgagtc
     3301 gccttttttt tgtctgtaga aaagtaagat gaggagcgaa ggcatgaaac catatcagcg
     3361 ccagtttatt gaatttgcgc ttggcaagca ggtgttaaag tttggcgagt ttacgttgaa
     3421 gtccgggcgc aaaagcccct atttcttcaa cgccgggctg tttaataccg ggcgcgatct
     3481 ggcactgtta ggccgttttt acgctgaagc gttggtggat tccggccttg agttcgatct
     3541 gctgtttggc cctgcttaca aagggatccc gattgccacc actaccgccg tggcgctggc
     3601 ggagcatcat gaccttgacc tgccgtactg ctttaaccgc aaagaggcaa aagaccacgg
     3661 tgaaggcggc aatctggttg gtagcgcgtt acaaggacgc gtaatgctgg tagatgatgt
     3721 gatcaccgcc ggaacggcga ttcgcgaatc gatggagatt attcaggcta atggcgcgac
     3781 gcttgctggc gtgttgattt cgcttgatcg tcaggaacgg gggcgcggcg agatttcggc
     3841 gattcaggaa gttgagcgtg attacaactg caaagtgatc tctatcatca ccctgaaaga
     3901 cctgattgct tacctggaag agaagccgga aatggcggaa catctggcgg cggttaaggc
     3961 ctatcgcgaa gagtttggcg tttaaagaaa ctcgccggat gaaaagtcat ccggcgtcat
     4021 attactgcaa ctgtgccgca attagcggcc agcgggcgtc aaaatcatcc gtcgggcggt
     4081 atttaaattc gctgcggaca aaacgtgaca gcataccttc acagaaggcc aggatctggc
     4141 ttgccagcag ggtttcatcg gtggtgtaac cttcaccctc acgcattctc ttttcacgca
     4201 atacctgacg cagctgcgct tcaatacgct cgaacagctg gttgatgcgc ccttgcaggc
     4261 gatcctgttc aaacattagc gcatgaccag tgaggatgcg ggtcaggcca ggattacgct
     4321 caccaaaacc gagaagcagc aacacaatca gacgcaggcg cgctgtggtg tctttctcat
     4381 ctttcagaat caggttgatg cgagtaatca ggctatcttc gataaactca atcaggctat
     4441 cgaacatgcg ggtcttactg gggaagtggc gatacagtgc cgcttcggaa acgccgacag
     4501 aggcggccag ttttgccgtc gtgatacgtt ggcttccatc gctggattcc agcatcagcg
     4561 ccagagactg aagtatttcc tcgcgacggt tccttttcgc agtttgtttt tctgccatgt
     4621 tacaaaatac ccctgaaaat aagcacttgc caggcggcac ccacgctatg accgcaaacg
     4681 aaatgtttgc ggctatgtta tgacgttatt cggatgcgta tgtgttactg acgaccagag
     4741 tgaccaaagc cgccttcacc gcggtcggtg gcgtcgaaat cttccaccag attaaattca
     4801 gcctgtacta ccggaacaaa aatcatctgg gcgatgcgtt cgccaggttg aatggtgaag
     4861 ctgtcctgac cacggttcca cacggaaatc atcaactggc cctgatagtc ggaatcgatc
     4921 aatcctacca ggttaccaag cacgataccg tgcttatgtc ccaatccgga gcgcggcagc
     4981 atcattgccg ccagtgaagg atcggcaata tgaatcgcca gcccggtcgg aaccagcgta
     5041 gtgtcacccg gagccagttc tacggcgtcg tcgagacagg cacgcaggtc aagtccggca
     5101 gagccagagg tggcataagt cgggagcgga aattccttcc caacgcgcgg gtccagaatc
     5161 ttaacgtcga tttttttcat cataacgggt cacgatctcg tcgagtaata gttggccaag
     5221 gagctctttg cgctcaagcg gtaagacttt atctccgtcc tgccagaaaa ggtgtaatgc
     5281 gttgttttcg ctgttaaatc cttgagttgg ctgggaaaca tcgttcgcgc agatcagatc
     5341 aaggttttta cggatacgtt tttgccgggc gtattcttcc acattatttg tttcggcggc
     5401 aaatccaacg acgtagggtc gatggtcttt tagtgcggca acgcctgcga cgatatcggg
     5461 gtttttaacc atttttattg ttaattcatc accctgcgtg gcctgctttt tgattttttc
     5521 tggggccacg gtagctgcgc gataatccgc cacggcggcg cagccgataa aaatattttg
     5581 ctgctgtaca gaagcattca cggcggcttc catttccagc gcggtcatca catcaacacg
     5641 tttaacaaac ggtggcgtcg gtagtgaaac cggacctgat accagcgtga cgttcgcgcc
     5701 acgacgggca gcggcggcgg cgatagcaaa acccatcttg ccggagctgt gattagagat
     5761 ataacgcacg ggatcgagcg gttcacgcgt cggaccggcg gtaatcataa tgttcagatg
     5821 tttcaggtcg ttgacgggcg aaaaatgcgc caccgccata tccacaatgg ttaacggatc
     5881 gagcattcgc ccaggaccga tatcaccaca agcctgactg ccactgtctg gcccccagat
     5941 gagcaaacca cgggaagcaa gcccctctaa attatgctgc gtggcagcgg cacggtacat
     6001 ctgctggttc atggcgggga gcacggctac aggcgcaggt gtagccagac aaatcgtcga
     6061 taccaggtca ttcgccattc cggcagcaac acgggcaatc aaatctgccg tggcaggggc
     6121 gagaatcact aaatcagccc atttacccag ctcaatatgg cccatagcgg cttcggctgc
     6181 cgggtccagc aaactgtcgg aaacgggata accagaaacc gcctgcaagc taagtggggt
     6241 gataaaggct tttgccgctt cggtcatggc tacgcggacg tcggccccgc gatcgcgcaa
     6301 acgacgcacc agttcagggg ttttataggc agcaataccg ccgctaacgc cgagaacgat
     6361 ttttttaccg gccaggctca tgatgatttt tcctgttggg tgacaccaga agttggcgat
     6421 tttatcacaa tacttttgtt gtcgtgcctt cacctgagaa tcactttgcg aggcgctttc
     6481 caggattgaa aactggccgt cgatttaacg gaacggctat gacaggatgc gagcaccaca
     6541 aaggaggtga aggtgaaaaa caattcacag ctgttgatgc cgcgcgaaaa aatgctgaag
     6601 tttggtatta gcgccttaac ggatgtcgag ctgctggcgc tatttctgcg taccggaacg
     6661 cgcggtaaag atgtattaac cctggcaaaa gagatgctgg agaatttcgg ctctctttat
     6721 ggcttgttaa cctctgaata tgaacaattt agtggcgttc atggaattgg cgtggcgaaa
     6781 tttgcccagt taaaggggat tgctgaactg gcgcggcgtt actacaacgt gcggatgcgt
     6841 gaagaaagcc ctttactcag cccggagatg acgcgggaat ttttacaaag ccagcttacg
     6901 ggtgaggagc gggagatctt tatggtgatc tttctcgact cacaacaccg ggttataacg
     6961 catagccgtc ttttttccgg cacgctaaac catgttgaag tccatcctcg ggaaattatc
     7021 cgcgaagcga taaaaataaa cgcctcggcg ctgatccttg cacataatca cccttcgggt
     7081 tgtgctgaac ccagtaaagc ggataaactc attactgaac ggataataaa gagttgtcag
     7141 ttcatggatt tacgcgtgct cgaccatatc gtgattgggc gtggagagta tgtttctttt
     7201 gccgaacgcg gctggattta acccgctatg cgcgatcctt cgggatcttt gtctgttcgg
     7261 gacttgagca catcgctgag tcagcgtata ctacgccacc tttgagaatc tcgggtttgg
     7321 catttgggcc tggcaatcga gagttcacat agaactgcga tgactgggct gtaaagcctg
     7381 acgaggcgcc aataccccat acgaagctcg agctaatttg atttttggag aatagacatg
     7441 tcccgagtct gccaagttac tggcaagcgt ccggtgaccg gtaacaaccg ttcccacgca
     7501 ctgaacgcga ctaaacgccg tttcctgccg aacctgcact ctcaccgttt ctgggttgag
     7561 agcgagaagc gttttgtcac cctgcgcgta tctgctaaag gtatgcgtgt aatcgataaa
     7621 aaaggcatcg atacagttct ggctgaactg cgtgcccgta gcgaaaagta ctaagtactt
     7681 agaggaaata aatcatggct aaaggtattc gtgagaaaat caagctggtt tcttctgctg
     7741 gtactggtca cttctatacc actacgaaga acaaacgtac taagccggaa aaactggaac
     7801 tgaaaaaatt cgatccagtt gttcgccagc acgtgatcta caaagaagcg aaaatcaaat
     7861 aattctcgct ttgatgtaac aaaaaacctc gctccggcgg ggttttttgt tatctgcttg
     7921 cccccatatt gactgcatct gttcattcct ggagatgcta tgcctgaatt acccgaagtt
     7981 gaaaccagcc gccgcggcat agaaccgcat ctcgttggtg caaccattct tcatgcagtg
     8041 gtgcgcaacg gacgcttgcg ctggccggtt tcagaagaga tctaccgttt aagcgaccaa
     8101 ccagtgctta gcgtgcagcg gcgggctaaa tatctgctgc tggagctgcc tgagggctgg
     8161 attatcattc atttagggat gtctggcagc ctgcgcatcc ttccagaaga acttccccct
     8221 gaaaagcatg accatgtgga tttggtgatg agcaacggca aagtgctgcg ctacaccgat
     8281 ccgcgccgct ttggtgcctg gctgtggacc aaagagctgg aagggcataa tgtgctggcc
     8341 catcttggac cggagccgct tagcgacgat ttcaatggtg agtatctgca tcagaagtgc
     8401 gcgaagaaaa aaacggcgat taaaccgtgg ctgatggata acaagctggt ggtaggggta
     8461 gggaatatct atgccagcga atcactgttt gcggcgggga tccatccgga tcggctggcg
     8521 tcatcactgt cgctggcaga gtgtgaattg ttagctcggg tgattaaagc ggtgttgctg
     8581 cgttcgattg agcagggtgg tacaacgctg aaagattttc tgcaaagtga tggtaaaccg
     8641 ggctatttcg ctcaggaatt gcaggtttac gggcgaaaag gtgagccgtg tcgggtgtgc
     8701 ggtacgccga ttgtggcgac taaacatgcg cagcgggcaa cgttttattg tcggcagtgc
     8761 cagaagtaat tcatgcgcgc cggatcgcat accatccggc ataaacgcta cgctaacttc
     8821 gccatcagcg cctgatggac attctccggc aggaaatggg tgacatcgcc ctgatggcgc
     8881 gccacctctt tcaccaacga tgaagagata aacgaccact ctttcgacgg catcagaaac
     8941 acactttcca gttccggcat taagtggcga ttcatatgcg ccagctgcat ttcatattca
     9001 aaatccgcca ccgcacgtag gccacgaatc agcaccgtag cgtgttgatt acgggcgaag
     9061 ttcgccatca aatcactaaa cccaactact tccacgttcc ccagatgcgc ggttgcctgc
     9121 tgtgccagcg ccacacgctc ttccagggta aacatcggtt ttttactggg gctggcggca
     9181 atcgccagaa taacgtgatc gaacatttgc gtggcgcgcg tcacgatatc gatatgacca
     9241 ttggtaatgg gatcgaaagt acccggataa atcgcccgtt tttgcataac aacctcaatg
     9301 cgttttcggt ggcaggtaag gttccagcag ttgaagcagg cgctgtagcg cgccctggtt
     9361 ttgatacagt acttcaacgg catgacggcc atagaaacta cggtaatcgg cgtcggtgag
     9421 taaagaggaa acctcttttg caagcgtagt ggcatcggta acggtaatca gcccgcttgc
     9481 ctgctccagc cgcgcgcaaa tgtctttaaa attaaaagta tgcggcccca tcaataccgg
     9541 aatagcgtgt gcggcagctt ccagcggatt atgcccacca cgttcaacca gtgaaccgcc
     9601 aacaaaggcg agatcggcaa tgccgtacag taacatcaac tcgcccatcg tatcgccaac
     9661 cacaacctgc gtgctggtgg aggggacttc ccctgaagag cgtgtgatat agcttagtcc
     9721 agcctggcgg acaaggttaa tcgcatccgg gaagcgttct ggatgacggg gtaccaggat
     9781 gagcaataaa ttcgggaatt gctgtaacaa tacctgatgt gcggcgatca ccacactctc
     9841 ttcaccttcg tgagtgctgg tggcaatcca taccgggcgg tgtggtgccc actggcggcg
     9901 cagcgtcacg gctttagcag ccaactgcgg cgttacagaa atatcgaatt tcaggctacc
     9961 ggtaacggtt acctggttat tttttgcgcc cagcgccaca aaacgtgcac catcttcttc
    10021 attttgggcg gcaatcagcg taatacgacg cagcaagcga cggacgaatt tacccagttt
    10081 ggcataacct gcggccgagc gggcagagag tcgcgcgttc gcgatcacca gcggaatttt
    10141 acgtttatgt agcgccgcaa tcaggttagg ccatagttcg gtttccataa tcaacaccag
    10201 tttagggtcg actttattca ggaaacgatt aagtgcatcg ggcaggtcat acggcagata
    10261 aacgtgctga acatccttcc cgaaagccga ttgtacgcgc tccgaaccgg ttggcgtcat
    10321 ggttgtaacg gtaatcggta aatcaggata acgatgacgc agcgcgcgca ccaacgggat
    10381 cgccgccaga gtttcaccga cggagacgga gtgcagcata atgccgcctg gttttagcgg
    10441 atggcggtaa aaaccgtaac gttcacccca gcgttttcga taggccggag ccttacgtcc
    10501 gcgcacccag agccgtatcc agatcagcgg ctgaataagg tagagaaggg cggtgtaaag
    10561 caattcgagc atagtaaata gctgacttat ggatgtgctg gggattctat gtatttagct
    10621 gtggctttac cattactttt cccgtttttg acttaaatag caatttaccc gccaattctt
    10681 gctgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg ctgtacgtgc
    10741 aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt atttgattga
    10801 tttctttatt gaaaatcaac aatatgctta aaaatcattc gggtcggatt gccgcgaaag
    10861 aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag ctgttgagcc
    10921 tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac aaaaagagtt
    10981 acttgtggat aagctatttc gaagaatttt gctcattaag atgcgttttc atggggatat
    11041 gttattaact actcccgtca ttagttcgct gaaaaaaaat taccctgacg caaaaatcga
    11101 tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga ttaacgcgct
    11161 ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact tttttcatct
    11221 catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatctcaccg atcaatggat
    11281 ggttgctata ctggttcgct tattaaatgc ccgtgtgaaa atttcccagg attatcatca
    11341 tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc agggtggaaa
    11401 tgtggtggaa agtaacttat ccgtgctgac cccattgcga gttgattcgt tggtgaagca
    11461 gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac ttgatcatgc
    11521 tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct tcaaatgctg
    11581 ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt atgaagttgt
    11641 tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg cgcagggatg
    11701 ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac ttggtgcgtt
    11761 aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata ttgccgctgc
    11821 agttaatacg ccgctgatat cgctgtttgg tgcgacagac catattttct ggcgtccctg
    11881 gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa cgcgcgatca
    11941 gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca ttgctgctgt
    12001 cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc tttttgttta
    12061 tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc tcagacagtc
    12121 gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga ataccctgat
    12181 gtatttgaac tgatcaaagt gccggttaaa tcgcatacca atcacgggcg caatgcggag
    12241 tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt cgttggattc
    12301 aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc cgagaaagtc
    12361 gcgcaggaaa aaggcttttt ctatcacctg acgtcacgtt atcgccatta tgccgccttt
    12421 gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac agataagcaa
    12481 atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat tctgccaccg
    12541 gggatttatc ctgatcgtaa atatagccag cagccagcta atagccgtga aatcttccgt
    12601 aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc agacttcacg
    12661 cgtaaaggtg tcgatcgttc cattgaagca cttgcttcgt taccggattc gctgcgccac
    12721 aacacattgc tatatgttgt tggacaggat aaaccgcgaa aatttgaggc gctggcagaa
    12781 aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt ctcggaatta
    12841 atggcggcgg cggatttatt actgcatcct gcctaccagg aagcagcggg aattgtgcta
    12901 ctggaagcga ttactgcagg attaccggta ctaacaacag ccgtttgtgg ctatgcgcat
    12961 tatattgtcg acgccaattg cggcgaggct attgctgagc cattccgcca ggaaacattg
    13021 aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcctg ggcggaaaat
    13081 gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc ggcggatatc
    13141 ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat ggcgcggtaa
    13201 agatcctttt gaggaagtta aaaccttgca gggtgaggtt tttcgtgaac tggaaactcg
    13261 ccgcactctg cgatttgaaa tggcgggcaa aagctatttt ctcaaatggc atcgcggcac
    13321 gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat taggcgcaga
    13381 ccgcgaatgg agtgcgattc atcgactgcg ggatgtcggc gttgatacta tgtatggggt
    13441 ggcatttggc gaaaaaggta ttaatccgct gagcagaacc tcgtttatta ttaccgaaga
    13501 tctgacccca accatcagtc tggaagatta ctgtgctgac tgggcgacca acccaccaga
    13561 tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg atatgcatgc
    13621 tgcgggcatt aaccaccgtg actgttatat ctgtcatttc ctgctgcact tgcctttttc
    13681 cggtaaggaa gaggagttaa aaatttcggt aattgaccta caccgggcgc agcttcgcac
    13741 gcgcgttcca cgtcgttggc gcgataaaga tcttataggg ctttattttt cttcaatgaa
    13801 tattggcctg actcagcggg atatctggcg gtttatgaaa gtgtattttg ccgccccgct
    13861 taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa aagccacaaa
    13921 aatcagggaa agaacgattc gaaaatcgtt gtaatttccg gagagaaata tgagtgccca
    13981 ctattttaat ccacaagaga tgatcaataa gacaatcatc ttcgatgaaa ggccagcggc
    14041 gtcagtggca tcatcattcc atgttgctta tggcattgat aaaaactttc tttttggttg
    14101 tggtgtttca atcacgtcag ttttgttaca taacaacgac gtgagttttg ttttccacgt
    14161 ttttattgat gatatccctg aagccgatat ccagcgttta gcccaattgg cgaaaagcta
    14221 tcgtacctgt atccagatcc atctagtaaa ttgtgaacgg cttaaggcat taccgacgac
    14281 caaaaattgg tctattgcca tgtatttccg ttttgtaatt gcagattact ttattgatca
    14341 acaagataag atcctttacc tggatgctga tatcgcctgt cagggaaact taaagccgct
    14401 gataacaatg gatcttgcca ataacgttgc tgctgttgtt actgaacgcg atgctaactg
    14461 gtggtcgtta cggggtcaaa gtctgcagtg taatgaactt gaaaagggct actttaattc
    14521 aggtgtcctg ttaattaata cactagcgtg ggcgcaggag tccgtttctg ctaaagcgat
    14581 gtcgatgctt gctgataaag ccatcgtttc ccgtttcacc tatatggatc aagatatcct
    14641 taatcttatc ctgttaggga aagttaaatt cattgatgct aaatacaata cgcaatttag
    14701 tttaaattat gaattaaaaa aatcatttgt ttgtccaatt aatgatgaaa ccgtattaat
    14761 tcattatgtc ggcccgacaa aaccctggca ttactgggcc ggttatccaa gtgcgcaacc
    14821 ttttatcaaa gccaaagaag catcgccctg gaaaaatgaa ccgttaatgc ggccagttaa
    14881 ctcaaactat gctcgttatt gcgccaagca taattttaaa caaaataaac caattaacgg
    14941 gataatgaat tatatttatt atttttattt aaagataata aaatgaaatt gagaaaagtt
    15001 aaatgaatga atttataaaa gaacggtttt cgtatttagc agataataaa aaagaaaacg
    15061 ccccagagct aaatgtttcc tacggtatcg ataagaattt tttgtatggt gctggcgttt
    15121 caatttcttc cgttttgatt aataattcag atattaattt tgtctttcat gttttcactg
    15181 attatgtgga tgatgattat ttaaagtcat ttaatgaaac agcaaaacaa tttaatacct
    15241 caattattgt atatttaatt gaccccaaat actttgctga tctgccgacg tcacagtttt
    15301 ggtcatacgc gacatacttc agggtattgt cttttgaata tctgagtgaa agtatttcca
    15361 cactgctgta tctggatgcc gatgttgttt gtaaaggaag cctgaaacct ctcacagaaa
    15421 ttatatttaa agatgagttt gctgcggtca ttcctgacaa tgatagtact caggcggcat
    15481 gtgcaaaacg cctcaacatt cccgaaatga atggacgtta tttcaatgca ggcgttatct
    15541 atgtcaatct taaaaaatgg catgaagcaa atttgacacc gtatttactc aaacttttac
    15601 gaggggaaac taaatatggc tctcttaaat atttagatca ggatgcgttg aatatcgcat
    15661 ttaatatgaa taatatctac cttgcgaagg attttgatac tatttatacc ctgaaaaacg
    15721 aactttatga tcgtagtcat cgaaagtatc agcaaaccat taccgataaa acagtgttga
    15781 ttcactatac agggataact aaaccatggc atagctgggc tggatatccg tctgcatcat
    15841 actttaatat cgcgcgtgaa caatctccct ggaagaaata tcctcttaaa gaggcgcgga
    15901 ctgttgcaga aatgcagaaa caatataagc atctgtttgc ccatggtgag tatataaaag
    15961 gcataacttc attaattaag tacaagctta agaaataaat tactcttatg attacaagta
    16021 tacgttatcg cggcttctca ttttattaca aagataatga taataaatat aaagaaatct
    16081 ttgatgaaat actggcctat aattttaaaa cagtaaaagt attatgtaat attgatgata
    16141 ccaaagtatc gttgattgat acgaaatatg gtcgctatgt ttttaaagtc tttgctccga
    16201 aaacgaaaag gaatgaacgt tttttaaaat cttttgtaaa aggtgactac tatcagaatt
    16261 tgatcgttga aaccgatcgt gtcagaagtg caggtcttac ttttcctaat gatttttatt
    16321 ttctggctga gcgtaaaatt ttcaactatg ccagtgtttt tatcatgctt atcgagtatg
    16381 tcgagggcgt agagctcaat gatatgccga ttattccaga aaatgttaaa gcagaaatta
    16441 aggcttcgat ggaaaaattg catgccttaa atatgctatc gggcgaccca caccggggta
    16501 actttatcgt tagtaaagat ggtgtcagga taattgacct atccggtaaa agctgtacgg
    16561 cagagcgtaa agctcgtgat cgtctggcca tggaacgtca cctgggtatt gccaacgaaa
    16621 taaaagatta tggctactat tcagtaatct acagaaccaa actacgcaaa tttattaaaa
    16681 aattaaaagg caaagcgtaa accacacagt caaaacggaa ccaacatgga tttattagca
    16741 gagagtatta ctgaagtcgc tgtctctggg gaaattgcta acaccgatcg tgtgttaaat
    16801 atcgcttacg gtattgaccg taacttttta tttggtgcgg cagtatctat gcaatcagtt
    16861 gttatgcata acccggacct tgcggttaag tttcatctct ttaccgacta cattgatgaa
    16921 gattatctac aacgtgttaa tgcttttacc agcaaaaatg ctaacgttga agtaataatt
    16981 tataaagtct ccaatgcctt tattgatatc ttccccagcc tgaaacagtg gtcttatgca
    17041 acattcttcc gtttagttgc gttccagtat ttgagtgaaa ctattgaaaa tctgttatat
    17101 atcgatgctg atgtcatctg taaaggctca ttagctggat tgcttgatat taattttgat
    17161 gaggataagt tcgcagctgt tattaaagat gtgcctttta tgcaggaaaa accagcgaag
    17221 cgtctggcta tagagggact tccagggaat tatttcaacg ccggtgtagt atatctgcag
    17281 cttgaagcat gggcgaaaaa tgattttatg aataaagcca ttgctatgct ggcaagtgac
    17341 ccgcagcaca cgaagtataa atgccttgat caggatattt taaatattct gttctttggt
    17401 cattgtaatt ttattagcgg cgattatgat tgcttttatg gcattgacta tgagttaaaa
    17461 aataaaagcg atgaagatta taaaaagacc attaccgatg atactaagct gattcattat
    17521 gttggcgtaa cgaagccctg gaacgactgg acgaattatc cctgccagaa gtattttaat
    17581 gaggcttatc aggcttcttg ctggaatgat gtggcgttta ttccagccac gaatgaaaag
    17641 cagtatcaag tgaaatatca acatgcaaag aaaaatggtg atacgtttaa cgcttttatt
    17701 tacttcatta aatttaaatt aaataagtat aaaagaaaac tatttggata ataataaaaa
    17761 tcatggatgt ctgagttaca ataataatta aggatgagta ctatcatatt ttattagttc
    17821 aggcaagaga acacgatgag caatgattac cctttagtat ctataataat accgacgtat
    17881 aattcatctg attacattac tgaaactcta acgaaattag aaaaacaaac ttacccaaat
    17941 tttgaaattg ttattgttaa tgatggttct aaagataaca catcaaacgt tttgagagag
    18001 tatgggttaa cccactctcg attaattatt gtcaataaag aaaatggcgg tgtttcgtct
    18061 gccaggaata caggtatccg caaggcgcaa ggacagttta tatgttttat ggatgatgat
    18121 gatgagatag atcctaacta tctgctgaag atgtatacca gacaacatga gacgggaggg
    18181 gatgcgattt attgtgggct ttatggccat tatataaaag atggtgttac ttactcacct
    18241 ataaatacag agtttaatga aggatcttta cttttcgact ttttttataa aaaggttaga
    18301 ttccatatag ggtgcttgtt tataagaaaa caacttctgg aagagaataa tctttttttt
    18361 gatgaagatt tacgactagg agaagatctt gattttatct atcgactgct aattacatgc
    18421 gatatgtatg cggttccata ttatatgtat aagcataact atagagaaaa ttccttaatg
    18481 aactcatgta gaaccatcac tcattatcga catgagtcat ttgcgcacga aagaatctac
    18541 tcttctgtga tgcagttata caaaggtaac cggaaagaag aaattcatac attattgagt
    18601 caaaatagag cttatcataa aactcgttat ttatggaatg ttctacttaa tggtgatttt
    18661 aaactattga atcaattagt tgaaagcaat gaaaaagaat taagagattg taatcttcct
    18721 ggcaagagag ataagagacg agcaaaaata ttagcatcaa aaaattatat tctctggagg
    18781 atggtaagac tggtaaatag aaaaaagaat aaacgttaga tcttgcatta aataacccat
    18841 cctcgtagca taggttgaaa ttatatgtcg ttttgttgga atgaaattaa ttctggtata
    18901 aagtctttaa ttcttatatt atgtattttt tctttaatga ctttgtcttt atgggatgat
    18961 gttgcaacaa agtttcttca tgcagctgga attatatctg cattgtattt tcttgcgaca
    19021 ccaaaaaaaa caataactaa taatcctact ttgttaattt tcatctcatt atgtcttttg
    19081 ggtatcgtaa atatcatctg gtattcacat tataaagttt caggctctgt ttataccaat
    19141 gcatatcgtg gcccaatgga aactggaaaa attgccttgt gtagcgcctt tattttctta
    19201 gttctttttg ctaaaaatga gatgagaaca aaaataaaat ttgggaaact aattctgttc
    19261 gcatccctgg caacgcagtt actttttttt gcgcatgcca tgtggcaaca tttctattta
    19321 aacgtcgacc gtgttgcatt atcagcttcc cacgctacaa cagcaggcta catcatcctt
    19381 tttccttctt tactggcatc aattctcatt ttaaaatccg actttagaca taaaacaaca
    19441 ttatatacaa ttaacttcat gcttagctta tgtgctgtca tagtaactga gacgcgtgca
    19501 gccatattag tgtttccatt ctttgcgtta atattaatcg taatggatag ttatattaat
    19561 aagcgaatta attataagtt atattgtttt attgcgattg cattattagc aggtgtattt
    19621 tcttttaaag atacattgct tatgagaatg aatgacttaa ataacgattt agttaattat
    19681 tcgcatgata acaccagaac ttcagtcggt gcccgtctgg caatgtatga agttggctta
    19741 aaaacatatt ctccaatagg acaatcactg gaaaaacgtg cagaaaaaat acatgagcta
    19801 gaagaaaaag agcctagatt gagtggcgct ttaccttttg tagattctca tttgcataac
    19861 gatctcatag atacgttatc aacgcgtggt attcctggag ttgtattaac aattttagca
    19921 ttttcagcaa tactcatata tgccttaaga actgctaaag aaccttatat tttaatcttg
    19981 cttttttcac tactggtagt aggcctaagt gatgtaatac tcttttctaa accggttccg
    20041 actgctgtgt ttgtcaccat aatattgctt tgtgcttatt ttaaagcaca atcagaccaa
    20101 tatttattag ataagtaatg aatatatgaa ataaaatcag caaggaggct gattttattt
    20161 caaaattatc tattaagggg ttgtaattta aatcatagca gcgttttctt caatgaatcg
    20221 cttaacagca tttgctgtca attgagacaa ctcattcccc ggagccctac aaaccatttg
    20281 attcttccca tacccaccaa ttaatcccgg atcggttggt ccataaaccg tgatattggg
    20341 tctatccagt gccgccgtta aatggcttaa ccccgtatcc accgacacta caaatttagc
    20401 cccggccagc acgcgggcaa cgccttccag actcatcttc ggcaatactt caacataatc
    20461 aaatccttcc gccagtcgtt tcgcccgttc ttcctcatgc ggcgcgcccc acggaagttt
    20521 aatccgtatt cctgaatcag ccagtaaacc aatcaattct cgccagtgtt cttccggcca
    20581 gtgtttatca tcacgggtcg tcgcatgaag aaatacggca tattcgccag catctgtagg
    20641 caggttcgtc agaaaatgct gtgcgatagc ataatcgccc tgggtttgcg gtttgctata
    20701 gcccaaactt ttggcaaaca gttcgcgggt gcgttctacg gcgtgctgct gttttgcaat
    20761 atgatgctta caattgtaaa acaggctggc taaaggttcg cgagcggttt gccagtccaa
    20821 tccatgcttt acgccatgcg ccagacgtgt caccagtgcc gcgcttttta ccagcccctg
    20881 agcgtcgata actgcgtcat agttctctgc ttgtagcgct tcacgaaacg ctttgcgttc
    20941 agcttttatg ggggccgaga accaggcttt acgccagcga cgtattgcca caggaataac
    21001 tcgctcaacg gcagcgtgcc aggaaggaat ctgtgcgaac ccttcttcca ccacccagtc
    21061 aaacttaatc cctgggattg cctgctgggc atcagtgagt gcgggcaacg tatggagaac
    21121 atcgcccatc gacgatgttt taacgatcaa aacccgcatc cgtcaggctt cctcttgtaa
    21181 caatagcgtg ttgagttctt ccagtacgcg ctggggagta atgtcgatca agctctggtg
    21241 ataaccctcc gcagcgtcac ctttacgcac tttgtgatag ccggtaatca gacggatcac
    21301 gcgcgcttta tgggatagcg gcggtgtgaa gtccgggcta cttggaccat acagggcaac
    21361 cagcggacga ttgagcgccg ccgcaacgtg catcaggcca gaatcgttag tgacaatggc
    21421 tttacaggct gcaatcagga taaccgcttg atcaagctgt gtttcccccg ccaggttccg
    21481 acaccatgcc tgctgctcgg tattcaaagc ggcaagaatc tcattgcccg cttcatgatc
    21541 tttcgcagag ccaaacagaa ccacctgata accttcatca atcagctgct ttgccagttc
    21601 cgcatagtgg tagtgtggcc agcgttttgc cggaccaaac tccgcacccg ggcaaaagcc
    21661 aatcatcgga cgttctgatg aaagcgaaaa ttgattacag gtatatgatt tttcaccttc
    21721 gctcacctgc aactgcggcc ataacaatgg ctgcggcaga tcttgtgcgg tacgcataat
    21781 gcctttgtca taggccagcg cgacatagcg ttccaccatt agcggccagg cttctttatc
    21841 gagcacgcgt acatcgttga gtaaaccgta gcgcatctcg ccgcgccagc cggtgcgatg
    21901 aggaataccc gcgaagaaag gcactaatgc agatttgaag gagttgggta agacgtaggc
    21961 gcggtcgtag cgcttttcac gcaggctatg acccagtttg cggcgttcgc cgatttccag
    22021 cgctccgtga ccgagaggca tagggatagc ttcgttaact tccggcatcc gcgataataa
    22081 tggacggcac catgccggtg ccatcacatc gattatcgcc tggggatagc gcgcctggag
    22141 cgtgcgatag agactttgcg acatcatcat gtcgccaacc caagacgggc cgatcaccag
    22201 tattttcatg cagagctctt atgcgtcgcg attcagccag gccatgtatt ccgttacacc
    22261 ttcagcaacg gttttgaacg gtttgtcgta acccgccgcg cgcagatttg tcagatctgc
    22321 ctgagtgaac gcctggtagc ggcctttcag cttatccggg aacggaatgt attcgatctg
    22381 gcctttcttg tgataagcca gagtagcatc tgctacggcc tggaaggatt ccgcacgacc
    22441 agtaccgagg ttgaagatac cggaaacgcc attttccagg aaccacaggt ttacatctgc
    22501 cacgtcgccc acatagacga agtcacgttt gaagttctcg ctaccttcaa acagcttcgg
    22561 tgattcaccg ttgttaagct gagtgttgag atggaaagcg acgctcgcca tgctgccttt
    22621 atggccttca cgcggtccat aaacgttgaa atagcggaag ccaacaatct gcgagttcgc
    22681 ttcaggcagg atttggcgaa catattcatc aaacaggaat tttgagtaac cgtagacgtt
    22741 caacggtttt tcgtactcgc gggattcaat aaagtcggag gtgcgtccgc cgtaggtggc
    22801 tgcggaagaa gcgtacagga acgggatttc gcgctccagg cagtagtgca gcagctcctt
    22861 ggagtattga tagttgttat ccatcatata cttgccgtcc cactcggtgg tggaagagca
    22921 tgcgccttcg tggaaaatcg cttcgacatc gccgaactct tcgccagcca taatctggat
    22981 caggaagtct tccttatcca tatagtccgc gatatccaga tccaccaggt tcacaaactt
    23041 ggtgccgtct ttcaggttgt ccaccaccag aatatcggtg atgcctttat cattcagggc
    23101 cttaacgatg ttgctgccga taaagcccgc gccgccggta acgatgatca taactgtaac
    23161 cttcgaatta tggagtcaga gacaatctca gacacgaata cttctatcat atcactatac
    23221 ggcgttggct tcagccattc gccaaaagtg agattgatgg acgttattta tcctgaattt
    23281 tgcagaagtg ttaacgcgtc atctcgtcgc gacgtataag tttgggtaat atgtgctgga
    23341 atttgccctg tctggagaat cgcaatgcgt ggagaatttt atcagcagtt aaccaacgat
    23401 ctggaaaccg cacgggcgga agggttgttt aaagaagagc gcattattac gtctgcgcag
    23461 caagcagata tcactgtggc tgatggaagc cacgtcatta acttttgtgc caacaactat
    23521 ctcgggctgg cgaatcatcc ggatctgatt gcggcagcaa aggcgggaat ggattctcac
    23581 ggtttcggca tggcttcggt gcgttttatt tgcggcactc aggacagcca taaagagctt
    23641 gaacaaaaac tggcggcatt cctggggatg gaagatgcga ttctctactc ttcctgcttt
    23701 gatgctaacg gtggactgtt tgaaacgctg ctgggtgcgg aagacgccat tatctccgac
    23761 gcactgaacc acgcgtctat tattgatggt gtgcgtctgt gcaaagctaa acgctatcgc
    23821 tatgccaaca acgatatgca ggagctggaa gcacgtctga aagaagcgcg tgaagccggt
    23881 gcgcgtcatg tgctgattgc caccgatggt gtgttctcaa tggacggcgt gattgccaac
    23941 ctgaaaggcg tttgcgatct ggcagataaa tacgatgcct tggtgatggt ggatgactcc
    24001 cacgcggtcg gttttgtcgg tgaaaatggt cgtggttccc atgaatactg cgatgtgatg
    24061 ggccgggtcg atattatcac cggtacgctt ggtaaagcgc tgggcggggc ttctggtggt
    24121 tataccgcgg cgcgcaaaga ggtggttgag tggctgcgcc agcgttctcg tccgtacctg
    24181 ttctccaact cgctggcacc ggccattgtt gccgcgtcca tcaaagtact ggagatggtc
    24241 gaagcgggca gcgaactgcg tgaccgtctg tgggcgaacg cgcgtcagtt ccgtgagcaa
    24301 atgtcggcgg cgggctttac cctggcggga gccgatcacg ccattattcc ggtcatgctt
    24361 ggtgatgcgg tagtggcgca gaaatttgcc cgagagctgc aaaaagaggg catttacgtc
    24421 accggtttct tctatccggt cgttccgaaa ggtcaggcgc gtattcgtac ccagatgtct
    24481 gcggcgcata cccctgagca aattacgcgt gcagtagaag cattcacgcg tattggtaaa
    24541 caactgggcg ttatcgcctg aggatgtgag atgaaagcgt tatccaaact gaaagcggaa
    24601 gagggcatct ggatgaccga cgtccctgta ccggaactcg ggcataacga tctgctgatt
    24661 aaaatccgta aaacagccat ctgcgggact gacgttcaca tctataactg ggatgagtgg
    24721 tcgcaaaaaa ccatcccggt gccgatggtc gtaggccatg aatatgtcgg tgaagtggta
    24781 ggtattggtc aggaagtgaa aggcttcaaa atcggcgatc gcgtttctgg cgaaggccat
    24841 atcacctgcg gtcactgtcg caactgtcgt ggcggtcgta cccatctgtg ccgcaacacg
    24901 atcggcgtcg gcgttaaccg tccgggctgc ttcgccgaat atctggtgat cccggcgttc
    24961 aacgccttca aaatcccaga caatatctcc gacgacctgg cttccatttt tgatcccttc
    25021 ggtaacgccg tgcataccgc gctgtcgttc gatctggttg gcgaggatgt gctggtctct
    25081 ggtgcaggtc cgataggtat tatggctgcg gcggtggcga aacacgttgg tgcacgcaat
    25141 gtggtgatca ctgatgttaa cgaataccgc cttgagctgg cgcgcaaaat gggtatcacc
    25201 cgtgcggtta acgtcgcgaa agaaaacctt aatgatgtga tggctgaact gggcatgacc
    25261 gaaggctttg atgtcggtct ggaaatgtcc ggtgcgccgc cagcgtttcg taccatgctt
    25321 gacaccatga accacggcgg ccgtattgcg atgctgggta ttccgccgtc tgatatgtct
    25381 atcgactgga ccaaagtgat ctttaaaggc ttgttcatta aaggtattta cggtcgtgag
    25441 atgtttgaaa cctggtacaa gatggcggcg ctgattcagt ctggcctcga tctctcgccg
    25501 atcattaccc atcgtttctc tatcgatgat ttccagaagg gctttgacgc tatgcgttcg
    25561 ggccagtccg ggaaagtaat tctgagctgg gattaacacg aacaagggct ggtattccag
    25621 cccttttatc tggggataat ctgttgaata tgtaaaatcc tgtcagtgta ataaagagtt
    25681 cgtaattgcg ctgatctctt atatagctgt tctcattatc tctctaccct gaagtgactc
    25741 tctcacctgt aaaaataata tctcacaggc ttaatagttt cttaatacaa agcctgtaaa
    25801 acgtcaggat aacttcagag gtcgtcggta atttatgatg aacagcacca ataaacttag
    25861 tgttattatt ccgttatata atgcgggcga tgatttccgc acttgtatgg aatctttaat
    25921 tacgcaaacc tagactgctc tggaaatcat tattattaac gatggttcaa cggataattc
    25981 tgttgaaata gcaaagcatt acgcagaaaa ctatccgcac gttcgtttgt tgcatcaggc
    26041 gaatgctggc gcatcggtag cgcgtaatcg tgggatcgag gtggcgacgg gcaaatatgt
    26101 cgcttttgtc gatgctgacg atgaggtcta tcccaccatg tacgaaacgc tgatgaccat
    26161 ggcgttagag gacgacctcg acgtggcgca gtgcaacgct gactggtctt ttcgtgaaac
    26221 gggagaaacc tggcaatcca tccccagcga tcgccttcgc tcaaccggcg tattaaccgg
    26281 cccggactgg ctgcggatgg ggctttcttc gcgccgttgg actcacgttg tctggatggg
    26341 ggtttatcgc cgtgatgtta ttgttaaaaa taacattaaa tttattgccg gattacatca
    26401 tcaggatatt gtctggacga cagaattcat gtttaacgcg ctgcgtgcgc gatataccga
    26461 gcaatcatta tataaatatt atctgcataa tacgtcagtg agtcggttgc atagacaagg
    26521 gaataaaaac cttaattatc aacgtcacta tattaagatt acccgcctgc tggagaaatt
    26581 aaatcgaaat tatgccgaca aaattacgat ttatccggaa tttcatcagc aaataactta
    26641 cgaagcattg cgtgtttgcc atgcggtgcg caaagagccg gatattctta cccgccaacg
    26701 gatgattgcc gagatattta cttccggtat gtataagcgc ctgattacca atgtgcgcag
    26761 cgtgaaggtc ggttaccagg cgttactgtg gtctttccgc ttatggcaat ggcgcgacaa
    26821 aacgcggtcg caccatcgca ttacgcgtag cgcctttaat ttgcgctagc gttaaacttc
    26881 gccgcttcgg gctgtttgcc ccagccttgc cactgatgct ggaagtaaac gatcagcgtg
    26941 ctctggctga tgctttcgct taatacttca aagaagcgat tagcataaac aggttctatc
    27001 ggtttcttcg gtttgcacag cttcacgcca cggaacggat tacgcggtgc atcaggcaca
    27061 gcgtttttcg gcggtgtaga agtatcaacc tgcggttcat tcagcaagct gctggctttc
    27121 accagcgtaa tgtctggcgg caggttataa accatctgtt gtagcacgcg taccgttaaa
    27181 gggtgaggat gcccaatggc aatggttgaa ccgttgcgac gcgccagatc aattgcgcga
    27241 ttaaattgca cacggatgtc cgcttcgttc tgcgaatcgt cgaggaacac cttccgttta
    27301 ataaccttca cgccagtacc ttgcgcggcg cgcatcgcct gggtattacc gatggttacg
    27361 ctgtcgagga agtaaagatt gtaacgctcc agtgcctgca tcactttctg cataccaaac
    27421 aggttagagg tcatcttgct acccatgtgg ttgttgatcc ccacggcata gggcacgtta
    27481 ttgaccgcac tacgaataat gcgctcaatt tcgtcgctgc tcatctccgg gcgcagcgta
    27541 tttttctcca gcggctgttt actcagtggt gccatcggga gatgaatcaa cacttcgtgc
    27601 ccgctgttat gcgctttggt cgccatctct ctggcgtgcg gtgaatcggg taatacagcg
    27661 acggagatag cggagggcat cgccagcacc tggttttcgt tgtgcgggcg atacccaaaa
    27721 tcatcaatga cgatggcaag tttgccagca agtacggggg aggagagcgc caacagagcg
    27781 gcaaatgcaa gaacgttacg acgaaatgga aacaaaactt atcttcccaa ccacggctgt
    27841 ggattgaccg cctgaccctg gcggcgaatt tcgaaataga gtgaaggccg accctgaccg
    27901 ccactgctgc ccaccagtgc aattggctgg cccgcgcgaa cctgcgaacc aacgctcacc
    27961 agtgcgctct gattatagcc gtaaagactc atgtcgcctt taccgtgctc aaccaccacc
    28021 accagaccgt aaccttgcag ccagtcagcc agaatcactc gaccgtcggc aattgcttta
    28081 acttcagtac cttcagaggc accgataacc atccctttcc agcgtaattc accctgtagc
    28141 tgctcgccat agcgatgcag cgtcggcccg cgaaccggcc agaatgcctg accacgcggc
    28201 gcacccagac caccagttcg ggacatcagc gatttttcgc tttcggtcgg tttgtaggtg
    28261 gtgcctttgc gcgtcgcttc tttctggcgg tcgcgaaccg cctgggcctc acgcgcttct
    28321 cgttcagcac gcgctttcgc cgcggcttcc gcacgggcaa tgctgttacg cagacgggat
    28381 tcgttggcac gcagttcact tagctgctgc tgaccttgct ggatggaaga ttccagcccc
    28441 gccagcgtct ttttacgctc gttcagagcc tgagtcagtt tcgcctgttg ggcgcgctgt
    28501 tcatataaca gcgtttgttg ctcgctttgt ttctcttcca gttcagcacg ctgcatggcg
    28561 acttcttcac gcgtttgctt caactgggca atggtttctt gtcgcgcctg gttgagatag
    28621 ccgaaataag cctgtaaacg ctgtccacac tggctttctt caccgctgag aattagctga
    28681 ataccggtat gctcgccctg acggaatgcg gcatccagtt gtgcggcgag gctgcgctcc
    28741 tgggcggctt tttgctgctc cagtttggca atcgacgcgt tcatctcatc aatctgttta
    28801 ttgagttgat tgagcgtgtt ttgcgtttcg cgcagcttac gggtggcttc agagatcgct
    28861 tcttcctgct ttttcaattg tgcgagcagg cttgcgcgtt gttgttgctt ttggcgtacc
    28921 gcgcgctctt ttgcggcgat atcggcctga atagatttga gttggtcacg ctcatccgcg
    28981 tgggcggaaa aggcgcacaa caatacgcca gcgctaagaa cgctggcgta gatgatgggc
    29041 ctgattgcaa acctgcgcgg tttcacggcc cgtgtcatgg tattaatcgc ctttcccctc
    29101 atggggaggg attattccac gatgaacagc ggcttaccag tcatctcttg cgggatttcc
    29161 atacccatca gcgacaacat ggtcggcgcg atgtcagaaa gtttgccgcc tgcaaccgct
    29221 ttcacgttct tatcaccaac gtaaatcagc ggaactggca ggttggtgtg tgccgtgtgt
    29281 gcctgacccg ttgccggatc gcgcatctgt tcagcgttac cgtggtcagc ggtgatcagc
    29341 agttgtccac ccacggactc aaccgctttc gcgacttctt ccacgcagtg atccagtgct
    29401 tcaaccgctt taaccgccgc ttccatcacc ccggtgtgac ctaccatgtc gccgttcgga
    29461 tagttacaga taatggtgtc gtatttgccg ctcttgatgg ccgcaaccag tttttcggtc
    29521 agctctgcgg agctcatttc cggttgcaga tcgtaggtag ccactttcgg tgagttgatc
    29581 agaatgcgat cttcgccttt gaatgactct tctacgccac cgttgaagaa gaaagtaacg
    29641 tgggcatatt tttcggtttc ggaaatacgc aactgagttt tgtcgttttt cgccatccac
    29701 tcgccgaagg tgttaaccag ggaagcgggt gggtaagcaa ccgcagtttt gatgtcagcg
    29761 gcgtattcgg tcagcatcac gaaatcgacg ttaaccactt tcttacgcgc gaagccgtcg
    29821 aaatctgcgt ttacgaatgc acgagtgatt tcgcgcgcgc ggtcagcacg gaagttcatg
    29881 aaaatcagcg catcgccgtc ttccattgcc gcatctggct gaccttctgc acgaataacg
    29941 gtcgctttca cgaactcgtc gttttcgtcg cgagcataag ctgcctgcaa accagcaacg
    30001 gcagtatcgg cctggaactc accctgcgcc agagtcagca ggtcataggc tttttcaacg
    30061 cgatcccaac ggttatcgcg gtccatcgcg tagtaacgac caatgatgga cgctacgcgg
    30121 cctttaccca gcgcagcaaa tttctcttca aattttttca gcgaggattc agcactgcgc
    30181 ggcggggtgt cgcgaccgtc aaggaatgcg tgcaggtaga ttttttctgc gccgcgttca
    30241 gctgccagtt ctaccatcgc catgatgtga tcttcgtggc tgtgtacgcc gcctgcggag
    30301 agcagaccca taatgtgtac tgctttacct gcgtttttcg ctttatctac cgcgccagtc
    30361 agcactggat tagcaaagaa agcgcgatct ttgatttcaa cgtccagacg agtcaggtcc
    30421 tgatacacga tgcggccggc acccaggtta acgtggccta cttcggagtt acccatctga
    30481 cggtcaggca gaccgacttc cagaccggaa gcgtcgatca gggtatgcgg acgattggcc
    30541 cacagtgcat ccattaccgg ggttttagca ctaaaaatgg cgttatcctg ctgttcttca
    30601 cgatagccat agccatccag aatcaccagt accataggtt ttttagaaac cgacattgcg
    30661 acaacctcat actcaagagt caaaatttgc gtaattttac tacagctgaa tcgataaaat
    30721 agcctctgaa gatcaaaata tacgaccgct cgcggcaagc cgatgccaga tttgcgcttt
    30781 tttttcgtcg tgccccgcaa aattgcattc cagttaacgc gctggctgta tttgccgcac
    30841 cgcgcaggta tactcctttc ctggtttttt taatcactac gtcgggagtt gttacccccc
    30901 atgcaagaaa ttatgcaatt tgttggccgt catcccatac tgagtatcgc ttggatcgcg
    30961 ttactggtgg cggttcttgt gactacgttt aagagcctga cctcgaaagt gaaggtgatt
    31021 actcgtggtg aagctacgcg tctgatcaac aaagaagacg ctgtggttgt ggatttacgt
    31081 cagcgtgatg acttccgtaa aggccatatc gcaggttcta tcaatctgtt gccgagcgaa
    31141 atcaaagcca acaatgttgg tgagcttgag aagcacaaag acaaaccggt tatcgtggta
    31201 gacggttctg gcatgcagtg ccaggagcct gcaaacgcgc tgacgaaagc tggttttgcg
    31261 caagtattcg tgctgaaaga gggcgttgct ggctgggctg gcgaaaactt gcctctggtg
    31321 cgcggcaaat aatttacctg tcgtctttca tgatgcaggc cgttagctgc aattaggccc
    31381 ccagcgactt attgttataa gcttctgggg acactccatt ttgcagccgc cctgcaacat
    31441 gaaatttcag ggtaaaaaaa caggagctaa attatggcca atgttgaaat ctataccaaa
    31501 gaaacctgcc cgtattgcca tcgtgcaaaa gcactgctga gcagcaaggg cgtgagtttt
    31561 caggagttgc cgatcgatgg caatgccgcc aagcgtgaag agatgatcaa acgcagcggt
    31621 cgcaccacgg ttccccagat ttttattgac gcacagcaca ttggcggctg tgatgacttg
    31681 tatgcattgg atgcacgtgg tggactggat cccctgctga aataacgtgt gaacgttggc
    31741 attacattgc gcagtattta aggacaacac ttaagggttt tctacacatg tcagaacaaa
    31801 acaacactga aatgactttc cagatccaac gtatttatac caaggatatc tctttcgaag
    31861 cgccgaacgc gccgcacgtt ttccagaaag attggcaacc agaagttaaa cttgatctgg
    31921 atacggcatc ttcccaactg gcagatgacg tatacgaagt ggtactgcgt gttaccgtaa
    31981 cggcctccct gggcgaagaa accgcgttcc tgtgtgaagt tcagcagggc ggtattttct
    32041 ccatcgcggg tatcgaaggc acccagatgg cacattgcct gggcgcatac tgcccgaaca
    32101 ttctgttccc gtatgctcgt gagtgcatca ccagcatggt atcccgcggt acattcccgc
    32161 aactgaacct tgcgccggtt aacttcgatg cgctgttcat gaactatttg cagcagcagg
    32221 ctggcgaagg tactgaagaa catcaggatg cctgatgaac caacgtaatg cttcaatgac
    32281 tgtgatcggt gccggctcgt acggcaccgc tcttgccatt accctggcaa gaaatggcca
    32341 cgaggttgtc ctctggggcc atgaccctga acatatcgca acgcttgaac gcgaccgctg
    32401 taacgccgcg tttctccccg atgtgccttt tcccgatacg ctccatcttg aaagcgatct
    32461 cgccactgcg ctggcagcca gccgtaatat tctcgtcgtc gtacccagcc atgtctttgg
    32521 tgaagtgctg cgccagatta aaccgctgat gcgtcctgat gcgcgtctgg tgtgggcgac
    32581 caaagggctg gaagcggaaa ccggacgtct gttacaggac gtggcgcgtg aggccttagg
    32641 cgatcaaatt ccgctggcgg ttatctctgg cccaacgttt gcgaaagaac tggcggcagg
    32701 tttaccgacg gctatttcgt tggcctctac cgaccagacc tttgccgatg atctccaaca
    32761 attgctgcac tgtggcaaaa gtttccgcgt ttacagcaac ccggatttca tcggtgtgca
    32821 gcttggcggt gcggtgaaaa acgtcattgc cattggcgcg gggatgtccg acggtatcgg
    32881 ttttggtgcg aatgcgcgta cggcactgat cacccgtggg ctggctgaaa tgtcgcgtct
    32941 tggcgcggcg ctgggtgccg agcctgccac ctttatgggc atggcggggc tgggcgatct
    33001 tgtgcttacc tgtaccgaca accagtcgcg taaccgccgt tttggcatga tgctcggtca
    33061 gggcatggat gtacaaagcg cgcaggagaa gattggtcag gtggtggaag gctaccgcaa
    33121 tacgaaagaa gtccgcgaac tggcgcatcg cttcggcgta gaaatgccaa taaccgagga
    33181 aatttatcaa gtattatatt gcggaaaaaa cgcgcgcgag gcagcattga cgttattagg
    33241 tcgtgcacgc aaggacgagc gcagcagcca ctaaccccag ggaacctttg ttaccgctat
    33301 gacccggccc gcgcagaacg ggccggtcat tatctcatcg tgtggagtaa gtaatgtcgt
    33361 gtgaagaact ggaaattgtc tggaacaata ttaaagccga agccagaacg ctggcggact
    33421 gtgagccaat gctggccagt ttttaccacg cgacgctact caagcacgaa aaccttggta
    33481 gtgcactgag ctacatgctg gcgaacaagc tgtcatcgcc aattatgcct gctattgcta
    33541 tccgtgaagt ggtggaagaa gcctacgccg ctgacccgga aatgatcgcc tctgcggcct
    33601 gtgatattca ggcggtgcgt acccgcgacc cggcagtcga taaatactca accccgttgt
    33661 tatacctgaa gggttttcat gccttgcagg cctatcgcat cggtcactgg ttgtggaatc
    33721 aggggcgtcg cgcattggca atctttctgc aaaaccaggt ttctgtgacg ttccaggtcg
    33781 atattcaccc ggcagcaaaa attggccgtg gcattatgct cgaccacgcc acgggcatcg
    33841 ttgtgggtga aacggcggta attgaaaacg acgtatcgat tctgcaatct gtgacgcttg
    33901 gcggtacggg taaatctggt ggtgaccgtc acccgaaaat tcgtgaaggt gtgatgattg
    33961 gcgcgggcgc gaaaatcctc ggcaatattg aagttggtcg cggcgcgaag attggcgcag
    34021 gttccgtggt gctgcaaccg gtgccgccgc atacaaccgc cgctggcgtt ccggctcgta
    34081 ttgtcggtaa accagacagc gataagccat caatggatat ggaccagcat ttcaacggta
    34141 ttaaccatac atttgagtat ggggatggga tctaatgtcc tgtgatcgtg ccggatgcga
    34201 tgtaatcatc tatccggcat acagtaacta atctctcaac aacgctcccg gatatcccag
    34261 ctgccgccag gcttcataca ccactaccga caccgcattg gacagattca tgctgcggct
    34321 gtccggcacc atcggaatgc gaattttttg ttcagcgggc agggcatcaa gaatgctcgc
    34381 tggcaggccg cgtgtttccg ggccgaacat cagataatcg ccatcctgat agcttacggc
    34441 gctgtgagca ggcgtacctt tcgtggtgag ggcaaacagg cgctggggat tttctgcttc
    34501 gaggaacgcg cgatagtcat gatgacgcgt aacggcggta aactcgtgat agtccagccc
    34561 cgcgcggcgc aggcgcttat cgtcccaggc aaatcccatc ggttcgatga tatgcagacg
    34621 aaagccggta ttagcgcaaa gacggatgat gttgccagta tttggcggaa tttctggttc
    34681 gtaaagtacg atgtttagca tactgccccc ttagtgcggg ggcaggatag cagaaaattg
    34741 gtgaaccgtg cggccttttc ccctcacccc ggccctcccc ctgtctcttg atcaggagat
    34801 ctgatcaaga gacagctccc tgagggagag ggtt
//
LOCUS       DAJTOR010000024        34582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTOR010000024 DAJTOR010000000
VERSION     DAJTOR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.024"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..950)
                     /gene="istA"
                     /locus_tag="OO249_001024"
                     /pseudo
     CDS             complement(1..950)
                     /gene="istA"
                     /locus_tag="OO249_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1566..1703
                     /locus_tag="OO249_001025"
     CDS             1566..1703
                     /locus_tag="OO249_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635399.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            2303..3082
                     /gene="ipaJ"
                     /locus_tag="OO249_001026"
     CDS             2303..3082
                     /gene="ipaJ"
                     /locus_tag="OO249_001026"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5635400.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(4026..4955)
                     /gene="virB"
                     /locus_tag="OO249_001027"
     CDS             complement(4026..4955)
                     /gene="virB"
                     /locus_tag="OO249_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5635401.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(5172..5408)
                     /locus_tag="OO249_001028"
     CDS             complement(5172..5408)
                     /locus_tag="OO249_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5635402.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(5412..7313)
                     /gene="ipaA"
                     /locus_tag="OO249_001029"
     CDS             complement(5412..7313)
                     /gene="ipaA"
                     /locus_tag="OO249_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5635403.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNDLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDAVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(7322..8320)
                     /gene="ipaD"
                     /locus_tag="OO249_001030"
     CDS             complement(7322..8320)
                     /gene="ipaD"
                     /locus_tag="OO249_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5635404.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSKK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELTKLKEKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(8371..9462)
                     /gene="ipaC"
                     /locus_tag="OO249_001031"
     CDS             complement(8371..9462)
                     /gene="ipaC"
                     /locus_tag="OO249_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5635405.1"
                     /translation="MEIQNTKSAPILYTDISTKQTQSSSETQKSQNYQQLAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTITPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRAYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            complement(9482..11224)
                     /gene="ipaB"
                     /locus_tag="OO249_001032"
     CDS             complement(9482..11224)
                     /gene="ipaB"
                     /locus_tag="OO249_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5635406.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LAANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPENKKLRREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGN
                     VASAVFQNNASKNLADLTLSKYQVEQLSKYIGDAIEKFGQLQEVIAELLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(11230..11697)
                     /gene="ipgC"
                     /locus_tag="OO249_001033"
     CDS             complement(11230..11697)
                     /gene="ipgC"
                     /locus_tag="OO249_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5635407.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11755..12381)
                     /gene="ipgB1"
                     /locus_tag="OO249_001034"
     CDS             complement(11755..12381)
                     /gene="ipgB1"
                     /locus_tag="OO249_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5635408.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(12397..12786)
                     /gene="ipgA"
                     /locus_tag="OO249_001035"
     CDS             complement(12397..12786)
                     /gene="ipgA"
                     /locus_tag="OO249_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5635409.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12799..14283)
                     /gene="icsB"
                     /locus_tag="OO249_001036"
     CDS             complement(12799..14283)
                     /gene="icsB"
                     /locus_tag="OO249_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5635410.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEV
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            14597..16213
                     /locus_tag="OO249_001037"
     CDS             14597..16213
                     /locus_tag="OO249_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5635411.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTNYLNNKNWEPINKNINY
                     NGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKELNSKRGEPTKLLIRNSDGLLQEVKVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEILNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            16223..16585
                     /gene="ipgE"
                     /locus_tag="OO249_001038"
     CDS             16223..16585
                     /gene="ipgE"
                     /locus_tag="OO249_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5635412.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            16585..17043
                     /gene="ipgF"
                     /locus_tag="OO249_001039"
     CDS             16585..17043
                     /gene="ipgF"
                     /locus_tag="OO249_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5635413.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            17052..18167
                     /gene="mxiG"
                     /locus_tag="OO249_001040"
     CDS             17052..18167
                     /gene="mxiG"
                     /locus_tag="OO249_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5635414.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKSGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICETNKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            18175..18426
                     /locus_tag="OO249_001041"
     CDS             18175..18426
                     /locus_tag="OO249_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HCR5635415.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTSALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            18439..18732
                     /gene="mxiI"
                     /locus_tag="OO249_001042"
     CDS             18439..18732
                     /gene="mxiI"
                     /locus_tag="OO249_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5635416.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSHPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18738..19463
                     /gene="mxiJ"
                     /locus_tag="OO249_001043"
     CDS             18738..19463
                     /gene="mxiJ"
                     /locus_tag="OO249_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5635417.1"
                     /translation="MIRYKCFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            19460..19987
                     /locus_tag="OO249_001044"
     CDS             19460..19987
                     /locus_tag="OO249_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5635418.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIEWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19929..20624
                     /gene="mxiN"
                     /locus_tag="OO249_001045"
     CDS             19929..20624
                     /gene="mxiN"
                     /locus_tag="OO249_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5635419.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            20617..21024
                     /gene="mxiM"
                     /locus_tag="OO249_001046"
     CDS             20617..21024
                     /gene="mxiM"
                     /locus_tag="OO249_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5635420.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDENIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            21008..21436
                     /gene="mxiM"
                     /locus_tag="OO249_001047"
     CDS             21008..21436
                     /gene="mxiM"
                     /locus_tag="OO249_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5635421.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            21566..22198
                     /gene="mxiE"
                     /locus_tag="OO249_001048"
     CDS             21566..22198
                     /gene="mxiE"
                     /locus_tag="OO249_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5635422.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            22185..23885
                     /gene="mxiD"
                     /locus_tag="OO249_001049"
     CDS             22185..23885
                     /gene="mxiD"
                     /locus_tag="OO249_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5635423.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKMTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSQGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTRNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23904..24971
                     /gene="mxiC"
                     /locus_tag="OO249_001050"
     CDS             23904..24971
                     /gene="mxiC"
                     /locus_tag="OO249_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5635424.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24984..27044
                     /gene="mxiA"
                     /locus_tag="OO249_001051"
                     /gene_synonym="virH"
     CDS             24984..27044
                     /gene="mxiA"
                     /locus_tag="OO249_001051"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5635425.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            27057..27458
                     /gene="spa15"
                     /locus_tag="OO249_001052"
                     /gene_synonym="spaK"
     CDS             27057..27458
                     /gene="spa15"
                     /locus_tag="OO249_001052"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5635426.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            27462..28754
                     /gene="spaL"
                     /locus_tag="OO249_001053"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             27462..28754
                     /gene="spaL"
                     /locus_tag="OO249_001053"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5635427.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMATAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELLGETIR"
     gene            28847..29185
                     /gene="spa13"
                     /locus_tag="OO249_001054"
                     /gene_synonym="spaM"
     CDS             28847..29185
                     /gene="spa13"
                     /locus_tag="OO249_001054"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5635428.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            29172..30050
                     /gene="spa32"
                     /locus_tag="OO249_001055"
     CDS             29172..30050
                     /gene="spa32"
                     /locus_tag="OO249_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5635429.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            30044..30925
                     /gene="spa33"
                     /locus_tag="OO249_001056"
                     /gene_synonym="spaO"
     CDS             30044..30925
                     /gene="spa33"
                     /locus_tag="OO249_001056"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5635430.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30930..31580
                     /gene="spa24"
                     /locus_tag="OO249_001057"
                     /gene_synonym="spaP"
     CDS             30930..31580
                     /gene="spa24"
                     /locus_tag="OO249_001057"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5635431.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            31595..31855
                     /gene="spaQ"
                     /locus_tag="OO249_001058"
     CDS             31595..31855
                     /gene="spaQ"
                     /locus_tag="OO249_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5635432.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31855..32625
                     /gene="spa29"
                     /locus_tag="OO249_001059"
                     /gene_synonym="spaR"
     CDS             31855..32625
                     /gene="spa29"
                     /locus_tag="OO249_001059"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5635433.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            32632..33660
                     /gene="spa40"
                     /locus_tag="OO249_001060"
                     /gene_synonym="spaS"
     CDS             32632..33660
                     /gene="spa40"
                     /locus_tag="OO249_001060"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5635434.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            33673..33915
                     /locus_tag="OO249_001061"
     CDS             33673..33915
                     /locus_tag="OO249_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635435.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10724 a         5143 c         6970 g        11745 t
ORIGIN      
        1 tgtcgaagct caccagattt tcaccaggat gcacgtcata ctcttttttc tccggaggca
       61 gtgccagcat ggactgctgc tcttcgagcc agcgatcaca gggacgggcc tggattgttt
      121 catgctttcg ttggttagcg acatcgtgca gccagcgcag accgtggcgg ttggctgttt
      181 caacatcgac agtgctcccc atcgggcgca ggcgagtcat tagtgggatg taaaaactgt
      241 tccatccgtt ccaccttacc tttagtctgt gccctgaagg ggcgacacag tcggggagag
      301 aagcccatct ccttgccgaa ctgccacagc gaaggatgga accggtgctg accggtctga
      361 tatgcgtcac gttgcagaac cacagttttc atattgtcat acaacacttc gcgcggcaca
      421 ccaccaaaga agcggaacgc attacgatgg caggtctcca gcgtgtcata acgcatattg
      481 tcagtgaatt cgatgtacaa cattcggctg tatccgggaa cagcaacgaa cacgtgaagc
      541 ggtgagcgac cattacgcat agtgccccag tcaacctgca tctgtcgtcc gggttcagtt
      601 tcgaaccgaa cggcaggctc ctgctcctga ggaaccgaga gagaacgaat gaatgccctg
      661 agaatggtca ttccgccacg atatccctgg tctctgatct cgcgagcgat taccgttgcc
      721 gggattttgt aaggatgagc atcggcgatg cgttgacgaa tataatcccg gtattcatcc
      781 aggagtgaag caacagcagg tcgcggcgta tattttggcg gctcagattt tgcctgcaaa
      841 taacgtttaa cggtattgcg ggagagcccc agttctctgg caatcgcccg gctactcatt
      901 ccctgcttgt gcaggatttt aatttccata actgtctcaa aagtgaccat aagctctcct
      961 gaatcaggag agcagattac cccctggatc tgatttcagg cgttgggtgt ggatcactat
     1021 tgcaccgttc gtgacacttg ttgactgcct gtgcggcatc tggtatcaag gtggcgtgaa
     1081 gccggttcag gttctcatgc ggaacgttgg aacctatatc cccccgatac caaaggcgaa
     1141 atgtaagtga caaaatgcaa tgaacacgtt ctccgttaac tgaggagcat ggtgataaat
     1201 aaaggtaaat gcagaaggtg tagagtctcg cagatttctg acatcataga gcctaacatt
     1261 agatgcataa cgctacagat tgtagaatct ctacaggtga aatatacact ggttggcgtt
     1321 tatgtcgcaa aacatgtcat tcaggttcat ctcaccaata aggatatgag tgaagtggag
     1381 gataagtgag tctgctgtca gagttttctg gtgtatgtca gtaactgcga accatgtctg
     1441 cttgatatgg gagcttatgg aggctctctg tacaaggtta gggagctaac aaagcaggga
     1501 taaaaagtcc gcctgatgca ggcacggttc gttaaatctt ttgccattgg taacaaaatg
     1561 gtgcgatgaa tgttcaggct atctttatct tgatggtggt tcagctgttt ggtaaagaaa
     1621 tcgctgtaac aatagaagaa ctgcaggcag tactggtccc acatcgtttg ctcagtcaac
     1681 tggtgaagtt ccgtagcgcg taaattaatg ctttgcatga aacgttactg gagtggatga
     1741 aagaatacct gcatatatca ttactgcgtt ggattaccag tacaacagat tgaatgaact
     1801 ggacatgttg atagacggca ttgaaaagca gcttataagc atagtgcggc agagttaatt
     1861 ggagagcact tcgccttctg agtattaagt aatcatggtg gtacctgcgg atactatcta
     1921 ttcactgtgc tcggtcggca gccttactga ccaaggagtt gggggagttg ataacaaagt
     1981 tgaaaaaaca gctctcaacc aaggtggtta ttgtaacaat agcaaataag ctggtgtgac
     2041 ctgtatcaca ataaccgatt atggacgtaa atacgacaaa aatatgtcag tatcaggcgg
     2101 tattaattgc tgatgctgat ggacattaac tcttaataaa agggtggatg ctgaaaggtt
     2161 gttatggtga cggacgtgat gacgacggaa aagagtatga ggttttggat ttaaagttcg
     2221 ttcggggaat aaggacccgg tcagctaatt ctcatagtag ttcgcattgc tggatgcaat
     2281 actgtgctgc aattactcgt cgatgtcgga acaacggaag ccttgcaaac ggggatgtat
     2341 tcatacaggt gttatgttat atggggtttt attgcagggg gctataccaa gagaatatat
     2401 gatatctcat cagactgatg tgagggtaaa tgagaataga gtaaacgaac aaggttgttt
     2461 tttggcgagg aagcagatgt atgacaattc ttgtggagct gccagtttac tctgtgctgc
     2521 aaaggaatta ggggttgata aaataccaca atacaaaggg tctatgtccg aaatgacaag
     2581 gaagagttct cttgatttgg ataatcgttg tgagcgggat ttatacttaa taactagtgg
     2641 taattataat ccgagaatac acaaagataa tatcgcggat gccggttatt ctatgcctga
     2701 taagatagtc atggctacga ggcttcttgg attgaatgct tatgtcgttg aagaaagtaa
     2761 cattttttcg caagtaatta gttttattta tcccgatgca agggacttat taattggcat
     2821 gggatgtaat atagtacatc aacgagatgt gttatcttct aaccaaagag ttttggaagc
     2881 tgttgctgta tcctttattg gagttcctgt cggcctccac tgggtgttgt gtagacctga
     2941 tggaagctat atggatccag ctgtaggaga aaattattct tgtttttcaa ctatggaatt
     3001 aggtgcaaga agaagtaatt ctaactttat aggatatact aaaataggta tttccatagt
     3061 tataactaat gaggctttgt aaattgtcgt tttttatctg tgaaggagct tgaatatttt
     3121 ggggcggggg cataacaaat gatattactg agtacaaatt tagctatatt gggcggatta
     3181 gttgtgtggg aagacgaaat gtttctcttg aaataagtga actccctcaa ggaatgattg
     3241 tgtgcttaaa tatttaagtg ctggtaagtt tgagaaggct gacatatttt gcagagcaaa
     3301 cagtgattga cttggatgat atttttattt tagtcactcg ttgtaattgg agtatgcttt
     3361 attttatttg aaaatagatg tattttatac ccgcaattta aactgtaagc cctatgaggg
     3421 gtatggatat tataagttac tctttgacta agcataactt ttggggttag aaaaataaat
     3481 ctcttgttaa taattaatat gattttaaaa tcaaacttgt ttataattat aaaatcatgt
     3541 aagtcggtca ttttatggga agattaggag aataaaacag taattatatt tgagaaatct
     3601 gctaatatat tcttaaagct tttttgatac tgtgaacgaa gtctgttaat ggtggatttt
     3661 ttgtttatca ccatgtaaaa aattgagtag taaaagcgtt gactgctaat cctcgctgtt
     3721 tttattgaaa cgattaaaaa tacgctgaat attttcggcc agtcactcat cttcacataa
     3781 aattttgacc atcatgccgc atcccttagt tgcacaattc attttcaatg atatgaatat
     3841 tactaatttc accagataat gttctcgtgg tcagcagggt atgagttttc accgtaattt
     3901 taaggaaaca cagtgcgtgg ggattgaata acagatagcc gacggaatta ccccaccggc
     3961 tgaaagtcaa gttcaacgaa gatttttatt atctgaattg ggcagtttac atcagtgttc
     4021 gatgtttatg aagacgatag atggcgagaa attatatctc gaatagcttc atctatttct
     4081 ctctgaactg ttttgtttat tttagataat gtaaatatta gagttctgtt tgttttattc
     4141 tcttttcttt ttatgtaagt ccgtttgtct ttagaaataa ataacgagtc aacttttagc
     4201 gaagggtttt gctttctgtt tttgcttttc tttatgatgt ttaaaatttc ttttttatat
     4261 atgtccgggg gcaaatttgt atcaaggtct tttatttctt cctttaatat tggtagtgta
     4321 gaactaagag attcattagc cttttcaagt cctttataat aattgaataa tatcttgtag
     4381 tcattaaagt taagttctga cgcgattgga aatagagata ttatttcctg tggaacgctt
     4441 gctgcctgaa aggcacgagt gactttcgcg cgagacagat tctctttttt ggctatgtct
     4501 ttataggaca tccctgatac tttcagaaaa ttaagaccaa taccaagttc tcggatgcta
     4561 tgctcttttg ctgtttgtat atcgtttgct agttttctgg catcaagagt agatatatat
     4621 tcttttgaat atagaacttc aagatctgct cctgcatata ttgcagatgc tcttctacga
     4681 gtgccatcca gaatttcaat tctaccatca atctcccttc ctattacagg gaagaattgt
     4741 tgtagcttta tagtttttat gatatcggct aatgattctt ctgttaggga ttcttgatcg
     4801 cgtacattcg ttttttggtt gacgaaggtt aaatcttgga tccttttatg aggaatcttg
     4861 gctttgataa aggaaacttt attaccagaa tggagtgtaa actctttctt ttggccttcc
     4921 tttatactta acaagtcgtt gcacaaatcc accatatcac accctgttta ttcatattga
     4981 tttatattga caaaagttaa atgcagtgtg agcaagattg tatgtaactg ttgatgatag
     5041 tcaacggtag tcttaaaaat tactatttta atagctggat tgatgcaatt tatctttatt
     5101 ggataaaaga ataatattct tgtggagctc tgatgtgaga gctccataga tgaaaaaatg
     5161 cttatcaatt actatattga ttttgtacta tttttgacta cagaaataag atctccaata
     5221 gtaataatgt gttctaatgc agattcatct atacgtagat tgaactcaaa actaatcata
     5281 tccagcattt caatcagaga aagagaatcg gcagaaagat cttcaataaa tttagtttct
     5341 tcagatagtt tgctgtacgc tataccatag caaaaggcca ctattgataa tattttttct
     5401 tttatcatat tttaatcctt attgatattc tttaatactt ttgataggga agtggttaca
     5461 tcttttgctg ctttatatat attattgttt tcagctgtaa tatcattgtt attttttaaa
     5521 tcagatatat catctgtaag taattctgca gaggtatcgt caatttttga caataccttg
     5581 ctgagggcgc tagatacttc ttttgccttt tcatagatcg catgattttt atcaattgta
     5641 tctgcgttgg tttctgttac gtagtttatt gtatttgcat ctgtaacttt taatttgttg
     5701 tttattctgt gttctgttgt ggattttgta atgtcctttg atttttcctg taactctcgt
     5761 tcttgtgttc ctgatttagt tccaatgaga gatataagat tacttaggga gtttgttact
     5821 ttttttgaag cttcaaatat cgtttcccgt gttaaatatg tgtctccatt tcttgataaa
     5881 aaatcagtct gaagtacttt tgaatcagtg tttgagttga atttattgat ttgcgaagct
     5941 actctatgag aaagagaagc gctactttta gttgtatctg atgagttatc attactgctt
     6001 gtcactcctt ccgggtggag agggggggtg gttgaagagt tctgtatgtt cagaggcctg
     6061 tgtccccgag aaagagagtt atttgtagat ctttcttgac tactcgtagt taacaggtct
     6121 gaacctattc catccgaatt atctggagta tggttgaaaa ccttttctaa agctgaatta
     6181 tttgcaaatg tctggacagc atctgatata gagtgtggta gaacggaagt tactttttga
     6241 actagtgctc tttgtggatt tagtaatgag tttccgttac gggataatag atcgtttgtc
     6301 tgagaagtag gagactgtgc atcattttct gggttctcgt gatagctatt gtcatatact
     6361 ctgttgtcaa aaactctatt atcgtaagtt ctattatcat tatttatatg atagtgaatt
     6421 acaggttggg atgttttctc attggctccg ggggttggtc cgccatctgg catatcttcg
     6481 ggaggtgtag ggaggccatc ttggatagga tgcgaatctt ttatatcttc taaaatatca
     6541 ttaaggactt ttctcaacag ctcaggaaac acggctactg tatttaagtc tcttgacagt
     6601 ttacctatac caaatccttt actatcaata gtatttttac gttgattgcg caaactatca
     6661 aaggcaagtt ttgcctgtgc gtcaactaaa tcatctagtt tgagagatga aacagtttca
     6721 ttacttaact ttaattcagc aagaatatta ttcttgataa atgatgatac ttcagtattg
     6781 aggagttttt gtcccaatga taaagcttca ccagatgtgt gttgttgtgc taatagtgca
     6841 agtctgtgct caatgttttg tcgtaattga tggagtttct tattctttgc cgaccatggt
     6901 ccaaaggcag aatcttggga taatttatct tttataatta gttccgaata ctgtgcgaca
     6961 attttttgaa gtcccttttc gttttcaaaa ttaagatcat tttgatgtac taaggagtac
     7021 aaaacatttg aaaattcgag aatctcgctt ttggcatacg cattcattga gttggaataa
     7081 atgcttctta acacatcttc tttccctgag gaagaaaata aaaaatcaag acatttctga
     7141 ttaaaagacg ttgcaaagtt ttctttttca gacactgatg ctggtgtttt tagagaagtt
     7201 tgcgaactat ggatatcgga tattttgctt tttaactcgc tgtattctgt tgatgatggt
     7261 gaagttgcct tatataagaa tgttggcgct tgagtattat ttacattatg catggcgcac
     7321 ctcagaaatg gagaaaaagt ttatctgtat ctgtacatga gcttattgta ctactcaaaa
     7381 cctttactaa attatcaaaa atactattgg cattactgta tttttgaact aaagtttgaa
     7441 gattattttt cattgtttca tcttcggcag agaatccggc attccatgcc tgatattttg
     7501 cattatctag cacaacctcg ccatttccac ctagattatc taagcttttt aacatattgt
     7561 ctattggggt catgtttata ttgacaacat atcccccgtt tttttcagat accttgccga
     7621 ttgttccacc taattctgta agccatttat ttgcttgttc cttactaaca gtattatttg
     7681 ctggatatag cggtttatct ttatattttt ccttgagctt tgtcaattcg tctttaagcg
     7741 atttgacttg taatttcacg gagtttccgt cgttacctcc gggagagatc cagccggcaa
     7801 gactggaaag aacagcgcta aaatcttgat acatttgagt atatgaacta acggcatgtt
     7861 catatacttt cagatactgt tcattaatat tattgattga ctttgcaatt ttatcccaca
     7921 gttctctgtg agatatcatt tcatatccat caagctcggc ttctttcggg gctgaatgta
     7981 ataattctct tgcgtcttta ttaattggat attctttctt ggaaagaata tccaatagtt
     8041 gagcggattt gtttacatcc atgctaatct gagatgaatg taatgctatt tcttctagcg
     8101 atgttttagt caacgttttt tgtgagagat ctttctttaa tgcctgattt gttgttctga
     8161 tattatgaag ggtgtcgttg agcatagtaa gggaacttac aggatgagaa ctggtcgttg
     8221 tttttatatc agaattaact gtttcggttg atgaaccgtt ggtattgttt gggctgaatg
     8281 atgaggtgga aatactatta gtcagagttg ttatattcat ggttatttcc ttataggaag
     8341 agccatatat tattatggct cttggatata ttaagctcga atgttaccag caatctgact
     8401 ggctgtagaa ttccttgatt ggttgatgtt gtcaattata ttcaataatt tttgtattaa
     8461 ttgatttgtc gcttgggatg cttctttaga tacttgggat gcttcctctg cttgtttact
     8521 gctggcctga ctgattagtt gttcttcttc ttcaagagca gatgtataac gccctcctga
     8581 tgtggatata ttaccagcaa cggctgatgt ctccattgtt gcacggccaa tgttttgttt
     8641 ttgctgagca gaaagggtat tgagctcgta agctcttctc tgggtgtcaa ttttatcctg
     8701 caaagaaata tcgggagaac taagagaagt tttatgttca gttgacaggg atatattatc
     8761 tggtgccagt ttatttttac ctaaaatttt tgtgctagag ttagtttgtg gtgaggtgat
     8821 atttgtatct atttgtttat ttaaccctaa tttagaacct gcaagctctt tttcaagaga
     8881 ttgagcagtg gcaaggttct ttcttaaggc tcctttttgt tcgctaatcc ctgaatgcgt
     8941 ttttttggca ccgatacccg ttatacctac ttgtgtgacc gctccagtaa tgcttgatga
     9001 tagggctgct aggccttgcc gaacaatgtt ctctgcagct gattttgtag catcgaacgc
     9061 aatcaatgac aattgagagc ccaatctagt ttctgcagtg cggagagcag aaagtagaac
     9121 ggctacacta ataattaaag aaacagcatt agaagaaaga ctggaaatat ccagggtgtt
     9181 ctctggggta attgtgtgac tcatttctga aagatctttc atctttttgt cagtaaggcg
     9241 agcaatgatt tctgaatcat gctgaacctg ctctgataac tttaaaagtt gatcatcatt
     9301 taatgtagtt gttaatacgg gatttttacc gacattaagt ggaatatgtg ctgctaactg
     9361 ctgataattt tgtgattttt gtgtttcgga agaactttga gtttgttttg tagatatatc
     9421 tgtatataaa atcggggctg attttgtgtt ttgaatttcc ataacattct ccttatttgt
     9481 atcaagcagt agtttgttgc aaaattgctt ttgcaacatc agttctatta gcctgagagt
     9541 tggacattga ggctaacagc tctgcaatta cttcctgcaa ttggccgaat ttttctattg
     9601 cgtcaccgat gtattttgac agttgttcaa cttgatattt cgataatgtc aggtctgcta
     9661 gatttttcga cgcgttgttt tggaaaacag cagaagcgac atttcctccc gcttgtgtgg
     9721 cagagttaac actttctcct aataaaactg cttggtttaa atgggtggaa ataatttgtt
     9781 tggatattac ttcatcaccc gctgcaccaa gaaatttatt taatctggca acagcattag
     9841 tgattaaatc gtccagttga gaagaaaaat tcttgagaaa ctttggtata aggtctgtga
     9901 gggttttacc tattattttg ccaatattct ctgcaagttt tgctgctgcc tgtttaccaa
     9961 cagtggctac gagaacgact gctgcaacca agacaagagc gcctgcgatt gcccccagaa
    10021 tagagccaat cattttggct tttttcgagt cgacgcccaa gccttcgagc atttttgtaa
    10081 atgcatctga aaggagtttg attaagggtt caatgactgc tttcatgatc ggattcaggg
    10141 cttgttccat gaaggaattg ccggtcgctg cttgtactat agcatccgta accataagag
    10201 ctaaaccaac agctgccagt gctagagatg ctcctccaga aaaagctgct gcaacaacac
    10261 taacgatagt taaaagtgcc ccaagtattt tcccaacaca acccattact ctgttgagtt
    10321 cttctgcttt acgtacttca gcagcatact catcagattt tctctccatt tcagtttttc
    10381 ttgattcttg gagagactgg aatagagcca gatcattttt taaagattct tcattatttt
    10441 ttccaactag ttgaataaag gttgccatca attgagtaac actggcaagt ccggttaatg
    10501 atttctgctg ggttgatagc tgttcagcag atgctgtgtt tgaaaatgca gaaaaagagt
    10561 ctatttcttt ttcgagttgc attgatttat ctgtaagttt gctatgaatt gacagagttt
    10621 tctgctcaat caatgtcctg tctttaactg ctgcgtcttt tttgatagtg agttgtattt
    10681 cttcccgtct taatttttta ttttctggtg aatctggggc gagttcggat aatcttgttt
    10741 gaatttggtt aattttattt tctaggtctt ttatttttga atctgcgttt tttagtttat
    10801 taatttgttt ttcatagtct ctggttagtc cttcagtttc agatagaaga gtattaattt
    10861 tatcggagaa ttctaggttt ttttgctgtc ttgcctgttg ctgggacttc caggcagtaa
    10921 ttttatttgt taatgcagtt aaagattttt caccaagtat ttgaataagg tttccaatta
    10981 aaagcgttag ttgggaactt gcatttaatg actttggtgc tttaagttca gggattaata
    11041 tattcgaagt tgagtgtgca ttagttgtat taatattttt gttagcagca agatcagcaa
    11101 ttgtaagaga aaataatttg ttagctgcat catttgcagc ctggatagta ttgtctccaa
    11161 gctcagtgga agccaatatt ttggcaagag gcaaaccagt ggttgtggtg cttacattat
    11221 gcataataat tactccttga tatcctgaat tgcgtctaag tatgattgtg cttttatttt
    11281 taatttttca tcattgctgt gttgaattac gagttcgaag cactctttgg ctttcaaggg
    11341 ggctttcaac cgaagctggc attgtccagt atggaatacc ggtgtatagt cattttttcc
    11401 taatgcaaaa gcgacagcat aaaggtctgc tgcttgttgg aactgttctt ttatctgata
    11461 aatagctgcg agtcccataa tgtagtctac attgtaaaag tcgtatatac ataaaaatct
    11521 gaagaaaact tcagcttcct ctattcttcc tttgttgtaa aagtcataag catatgaata
    11581 aatgtcatcc atcatatcat caggaattgc attaatatct ttcagtgtag cgccagagtt
    11641 aattgcatca attactgcag tagagatgct ttcattttca gtgatattta aagacataag
    11701 gtctcctttt tgtctgcatt actttaataa gtataagatt taatataaac agatttaatt
    11761 tgtattgctt tgacggtata cagcctttag aacctctgcg cttatttcta tttgtaaaac
    11821 ttttgttgat tcattaaata ttttatcgta gacggaactg gatactagat ttgtaagatc
    11881 cttcatttgt gtgttaaaca aatccatgtc actgctataa cgttttaaaa tctttgctct
    11941 gaggccagat gaggcaatct cgtttagtgc tgtatgactg atagatgttt gtgctgattt
    12001 ttcatcaagt tttatatccg ggaatttttt ttcaatagct gcaaatatgt tttttttgtt
    12061 atctgaggtc aaaacaactc cattgtgtat ggccatttca tctatacttc tgtttatcat
    12121 tgaagagaca taagaggttc gctcttggct catccaacaa aatagttgat tccccgaatt
    12181 actatctttt attttgtcta tgacgctgtc cctgatttta tttatattca ccgggcatcc
    12241 aaaattcttc tgaggaaagc gttgttttaa gttaaaaaca gataatattt tctttacaag
    12301 cttattgtga cttaacgaat caaatgatgg gcgaggaatc gcgaattcaa cctgtggaag
    12361 tattttgttt agaatttgca tgataccccc tatatgttag ttcacttctg aagtgatgtt
    12421 tgcaatggtt agtttaatac aaaataaaaa ttcgtacaaa gattcaatga gttcatcagg
    12481 tgaaagatat tttttgtagt gtttcttggt caaaacacat tgatgagtat cagggagaag
    12541 acagatagaa gaaccataat ctctactcaa agagtagtta aggatgagta tgtgttctgg
    12601 attaataata ttttctgtaa aaagttcatg ttcattaaga agtccggtca aaaagatgta
    12661 ttcatcatta tctgttatac aaacaggtat ttgttcttcg agcaatataa aggcttgatt
    12721 tttatcgtta aaatcaagct ttgcacctgt tatttcataa agtttatcat atagtttgcg
    12781 acacatttct atggccttct atatattaga atgagagtta ttcaataaaa gcttattgac
    12841 ctgtaataag tcgtgaatgt aaagtttggt tccaagatct ggcgatttaa gagaattgta
    12901 ataatcaata gtttttattg acttttcgat aattacttct tttttatctg ttgctccggt
    12961 ttttaattcg tttaatacac gagacatatt gctgggtgct gattttacta agtatgagtg
    13021 gcaagcgttg atgaccgcat caaagtcttc tgaagataca ttttcagtag gatgtttgta
    13081 aagtgaaatt gataaaggct tgaatgtttt tggggtgtgg ataaggttaa ttttattgaa
    13141 acttttaaga tagctacgtc gtaattgagt caatctggaa agatcaaaat cagattcaat
    13201 atacttcatt atctcgttat aagactgatt gagactttca atgcgttgcc taactttatt
    13261 tgcatacgcg tatacattgt taggtgtagc aaccaattta acgtcaggga gtggaaagta
    13321 tatttcagaa tttcctgctt taagaacatt aagtgccata ccagcacagt tttcagattt
    13381 actgattaat ttataatttg ccaattgtct gaaagcatct gctttctttt ggcatataaa
    13441 ttttgacatg tttgtttcat caaggccaaa aaggttatag ctgattttac catcctttgt
    13501 tttattatca cctgataatg gaatgtatac tttatcagca ctgacccccc aataaccata
    13561 ggggtctttt ttttgattag ctctgggaaa aaatattttt ttgttgttat tttgatcttg
    13621 ttttgaattt aataagtttc taacctcttg gcctgcatca agtctttcgg ctgtttggtc
    13681 tgaaatcata tttagtttgt cttgtttata ggtatcgaca ctgtaacctg agcgtttttc
    13741 aaaaaggtag tttatttctg tatcttttgt aacattcccg ccgggatacc agctgagata
    13801 ggtatgagtt ggttcctgta gtaaattgtt ttttatactg atactggtat gccctaaatg
    13861 ggttatgcct ctatttcccc agcaacgaaa cactaaatgt ctttcaagat ttttggctgt
    13921 accgatgcca tgaaaactct ctgcggtatt caacattaat ttgatgtctt tacctgtaaa
    13981 agaatgttga attgaatttt tgactgcttc ataggcggca aacgcacttt ctgcagattt
    14041 ttcgctagct aatgtcttga ggaaaagcaa taaggatttt tgattcgcta gtcgataatt
    14101 ttttagttgt tcatttaata tctgtgaatt tatctttgca ttaatagtgc ttaatgttct
    14161 gaaaaagaga tcctttaggt tgaatttctg tgctaccaat ataggtccat gctctgtatc
    14221 ttcaacgcgt ataggccctt ttgtattgct tgcgtcaatg aaattgctaa ttttgagaat
    14281 catactttat taactctcca ttacgtggtg attgtgggga tgtaaaatgt atttgatata
    14341 taatatattc aattaataaa tttagaaact tgagcctgtt aacataatca aattttcttt
    14401 tgctgtacat aatatgtacc tcgtgagcat atgtagtgct ggtttcatca tgaaatccca
    14461 caagttaaag tgtctgatat atcaggctcg gagtgttata gaaaaagaga gaaccctgtt
    14521 gaataagtat cttttttgtt gtaaaacgag gtacaaaact gcaatcaatg ccccatttat
    14581 gactgaggat aattaaatgc acataactaa tttgggattg catcaggttt catttcaaag
    14641 cggagattcc tataaaggcg cagaagaaac cggaaaccaa aaaggtgtaa gcgtgatttc
    14701 atatcaaaga gttaaaaatg gagagagaaa taagggggtc gaagcgctta atcgattata
    14761 tttacagaac caaacaagct taacagggaa aggccttttg tttgcgcgtg acagagctgc
    14821 agttttttac gaggcaataa agttggcagg tggtgatacg tcaaaaatta aagccatgat
    14881 ggaacaatta gatacctata aacttggtga agttgataaa agaaatatta atgagcttaa
    14941 taaagtaata agtgaagaga taagagcaca gctaggcatt aaaaataaaa aggaattgca
    15001 atcggaaata aaacaaatat ttactaatta tttaaataat aagaattggg aaccgatcaa
    15061 caaaaatatt aattataatg ggaaaaatta tggttttcaa ttaacacccg cctctcatat
    15121 gaagataggg aataaaaata tatttgtcaa agagtataat ggaaaaggaa tttgttgtgc
    15181 ttctacgaga gagagtaagc atattgcgaa tatgtggctt tcaaaagtgg tggatgacga
    15241 ggggaaggaa attttttcgg gtatcagaca tggagttata tcagcctatg gattgaaaaa
    15301 aaactcatct gaaagagctg ttgcagctcg taataaagct gaggagttag caagcgcagc
    15361 attatatagc agacaagaat tattatcaca ggctttgtct ggtaaaacag tagatttaaa
    15421 gattgtttcg acttctcttc tgacgccgac cagtttaact gggggagagg agagtatgtt
    15481 aaaagatcag gtaaaggcat taaaagagtt aaactctaaa aggggggaac caacgaaatt
    15541 attaattcgg aatagtgatg gtcttctgca agaagtaaag gttaatctaa aagtggtgac
    15601 atttaacttt ggtgtaaatg aattagcgct aaaaatgggc ttaggctgga ggaatgttga
    15661 caagcttaat ggtgaatcaa tatgttcttt gttgggggat aatttcctca aaaatggtgt
    15721 gattggaggt tgggctgcag aagctataga aaaaaatcca ccatgtaaga atgatgtgat
    15781 atatctggct aaccagataa aagagattct aaacaagaag ttacaaaaaa atgataatgg
    15841 agagccgtat aaattgtcac aaagaatggc tctattggct tatactatag gtgctgtacc
    15901 ttgctggaat tgtaagagtg ggaaggacag aacaggtatg caggatgctg aaattaaaag
    15961 agaaataata agaaaacatg aaacagggca gttttctcaa ttaaatagta aattatcctc
    16021 agaagagaaa agattatttt ctactattct aatgaatagt ggtaatatgg aaatccaaga
    16081 gatgaatact ggtgtgcctg gaaataaagt tatgaaaaaa ttaccgctat cttcgttaga
    16141 gctgtcttat gctgaaagaa taggggaccc caaaatatgg aatatggtga aagggtattc
    16201 gtcatttgta taagaggaat atatggaaga tttagcagat gttatttgcc gagccttggg
    16261 tatcccttca attgataccg atgatcaagc aatcatgctt gatgatgatg tgttaatata
    16321 tattgaaaaa gaaggagatt caataaattt attatgtcct ttttgtgcat tgcctgaaaa
    16381 tataaatgat cttatatatg cgttgagcct aaattactca gaaaagatat gtttagctac
    16441 tgatgatgaa ggtggaagtc taatcgcgcg attagatctt accgggataa atgaattcga
    16501 ggatatatat gttaatacag aatattatat ttcgcgtgtg cgttggctga aggatgaatt
    16561 tgcgcgaaga atgaaggggt attaatgtcc cgtttcgtat ttatttttct gtgttttatc
    16621 ccatatttag ggagagctga ttgttgggat aaggctggtg aaaggtacaa cattccatcg
    16681 agtttgttaa aagcgattgc ggaaaaagaa tccggattta ataaatttgc agtcaatgtt
    16741 aataacaatg gaagtaaaga ttatggtata atgcaaataa atgactttca ttctaaaaga
    16801 cttagagaaa tgggatatac tgaggaaatg ctaattagtc atccatgtct ttctgtacat
    16861 tatgcagcaa agttattaaa tgagtttatg ctgatgtatg gaagagggtg ggaggctgtt
    16921 ggtgcctata atgcaggaac atctccgaaa aagaaaaaag aaagattaaa gtacgcagag
    16981 gatatctata aacgctattt gcggatagca gcagagagca agcagaataa tcgaaggata
    17041 taagaggatt tatgtctgag gcaaagaact caaatcttgc accattcagg ttattagtga
    17101 aactcacaaa cggtgtcggg gatgagtttc cgttatatta tgggaataac ttaattgttc
    17161 tcggacggac gatagaaaca cttgagtttg gaaacgataa tttcccagaa aatattattc
    17221 cagtaacaga tagtaaatcg gatggtataa tttatttggc cataagtaaa gataatatct
    17281 gtcaatttag tgatgaaaaa ggtgaacaaa ttgatatcaa ttcacaattt aactcatttg
    17341 aatatgacgg agtttctttt caacttaaaa atatgatgga ggataaatcg agagttcata
    17401 tactaaacgg aatgtataat aaaccctcgg tgtttttttt ctttgctgtt attgttgtgt
    17461 taattataat tttttccctc tctttaaaga aagatgaagt aaaagaaatt gctgaaatta
    17521 tagatgataa gagatatgga atagtcaaca caggacagtg caattatatt cttgccgaaa
    17581 cacaaaatga tgcggtatgg gcatctgtag ccttaaataa aagcggattt accaaatgca
    17641 gatatattct ggtgtccaac aaagaaataa atagaattca acaatatatc aatcaacgat
    17701 tcccatatat caatttgtat gtgttgaatt tagtgagtga taaagcggaa ttactggtat
    17761 ttttaagtaa agaaagaaat agttcaaaag ataccgaact tgagaaattg aaaaatgctc
    17821 tgatagttga atttccgtat atcaaaaata ttaaattcaa ctatcttagc gatcataatg
    17881 caagggagga tgctaaaggt attttcacaa aggtaaatgt ccagtataaa gagatttgtg
    17941 aaactaataa agttacatat tctgtcagag aagaactgac agatgaaaag cttgagctca
    18001 ttaatagact gatttctgat cataaaaatg tatatggaga tcagtatatt gagttttctg
    18061 tacttttgat agatgatgat tttaaaggta aatcatatct taacagcaaa gacagttatg
    18121 taatgttgaa tgataaacac tggttttttt tagataaaaa taagtgagga taaaatgagt
    18181 gttacagtac cggataaaga ttggactctg agttcattat ctgaaacttt tgatgatgga
    18241 actcaaacat tacaagggca actaacatcg gcactaaatg cattagctga aaatccttcg
    18301 aatccacagt tgctggctga ataccaaagt aaattatctg aatatacatt atataggaac
    18361 gcgcaatcta atactgtgaa agtgattaag gatgttgatg ctgcaattat tcaaaacttc
    18421 agataatagg gagcattcat gaattacatt tatccagtca atcaggttga tattatcaaa
    18481 gccagtgatt ttcaatctca agagatatca agcctggaag acgtcgtgtc ggctaaatat
    18541 agtgatatta agatggatac agatattcaa gtatcacaaa taatggagat ggtaagccat
    18601 ccagaatcat taaacccaga atctttggcc aagttacaga cgacgctctc aaattattca
    18661 ataggagtat cattagctgg cacgttagca agaaaaacag tttcggctgt tgaaacttta
    18721 ttaaagtctt aatttatatg attaggtata aatgttttat tttattcttg ttgctgatgt
    18781 tgattggatg tgagcaacgt gaagagttaa tttctaattt atctcaaaga caggcaaatg
    18841 aaataatatc tgtgctagaa cgccataata ttactgctag aaaagttgat ggaggtaaac
    18901 aggggatctc ggtacaagtc gaaaagggga catttgcatc ggcagttgat ttgatgcgca
    18961 tgtacgattt gccaaatccg gagagagttg atatctcaca aatgtttcct acagattcat
    19021 tagtgtcttc tccaagagct gaaaaggccc gtttatatag tgctattgag caacggctgg
    19081 aacagtcttt agtttctatt ggtggtgtta tttcggcaaa aatacatgtt agctatgatc
    19141 ttgaagaaaa aaatatatct tcaaaaccga tgcatatatc agtaatcgct atatatgact
    19201 caccgaaaga gtctgaacta ttagttagta atattaagcg atttttgaaa aacacctttt
    19261 ctgatgttaa gtatgaaaat atatctgtca tattaactcc gaaagaagaa tatgtttata
    19321 caaatgtaca acctgttaag gaaattaaat cggaattttt aacaaatgaa gtaatatatg
    19381 tatttctcgg gatggctgta ctagttgtca ttcttttggt atgggcattc aaaacagggt
    19441 ggttcaagag aaacaaaata tgataagaat ggatggaatt tataaaaaat atctttcaat
    19501 aatttttgat ccagcgttct atataaatag aaatcggtta aatttgcctt ctgaactgtt
    19561 agaaaatggc gtaatcagaa gtgagattaa taatctcata attaacaaat atgatctaaa
    19621 ttgcgatatt gaacctttaa gcggggtaac cgctatgttt gttgccaact ggaatttact
    19681 tccagctgtt gcttatttta tcggtagtca agagtctcgg ttaattaatc atagtgagat
    19741 ggttattagt tattatgggg gcaaaatcag taagcaaggg gaggccgcta ttcgaagtgg
    19801 tttttggcat cttattgagt ggaaggaaaa tatatctgtt ggtatatatg aaagaataaa
    19861 cttattattt aatccaattg ctttggaggg aaattatacg cctgtagaaa gaaatttgtc
    19921 ccgattaaat gaaggtatgc aatatgcaaa aaggcacttt accggtatcc agacatcatg
    19981 cctatgatgg tgttgttata aagagaattg aaaaggaatt atgtaaaacc ataaaagata
    20041 gagacactga gagtaagaag aaagcaattt gtgttataaa ggatgctacg aaaaaagctg
    20101 aatcactgcg tatagatgct gtatgcgatg gttatcaaat tggtatacag actgcttttg
    20161 agcatattat tgactatatt tgtgaatgga agttaaagca aaatgaaaac agaagaaata
    20221 ttgaggacta tataactagt ttgctttcag aaaatttaca tgatgagagg atcattagca
    20281 ctttattgga gcagtggctt agttcgttgc gtaatacagt aacagaatta aaagtggttt
    20341 tgcccaagtg taatttagca ctgaggaaaa agttagaact tgatctccat aaatatcgct
    20401 cagatgtcaa gattatttta aaatattctg aagggaataa ttatatattc tgtagtggta
    20461 atcaagtagt ggagtttagt cctcaggatg taatatcagg tgttaaaata gaacttgcgg
    20521 aaaaattaac aaaaaatgat aaaaaatatt tcaaagagtt agcacataag aaactgagac
    20581 aaattgcaga agacttgtta aaagagaatc ctgttaatga ttaatcaaat aaatgcaagc
    20641 aatgctctgc agcaaagatt aaatagtgaa gaatttgtta atttaaatga gagattatca
    20701 tcttcgcaat catttgatga gaatataatc tatgaaataa tgcaatattt ctcacagagt
    20761 gaattaaatt ccatagataa tgatgaactg cacaataaga ttgaacaatt attcaattca
    20821 cgttttcctt atcttacagc tgcacaaaaa agttcacttc ttaataaact catagatgca
    20881 aatcaatatg tcgatttgca tgagggattt tatgcatcat tgtccatata taacaatatt
    20941 gatttctata ttaagactac aaccttcgat tcattaatta gtgtctttga agcagggaga
    21001 gaggcagatg attcgacatg gtagtaataa gttgaaaata tttattttaa gtatattgct
    21061 attaacactg agtgggtgtg ctttaaagtc atcatctaat tctgaaaaag aatggcatat
    21121 tgttcctgta agtaaggatt atttttctat tccaaatgat ttattatggt cgtttaatac
    21181 aaccaataaa agtataaatg tttactctaa atgtattagt ggtaaggcgg tttatagttt
    21241 taatgcaggt aaattcatgg gcaactttaa tgttaaggaa gtagatgggt gcttcatgga
    21301 tgcacaaaag atagctatag ataaactatt ttctatgctg aaagacgggg ttgttttaaa
    21361 aggtaataag ataaatgata ccatccttat agagaaggat ggggaagtta aattaaaatt
    21421 aattcgaggg atataattgt attgtgagta aatataaagg tctaaataca agtaatatgt
    21481 tttacattta ctctagtgga catgaaccag ttaacgttga gcttgtaaaa gataaagaac
    21541 gtaacataat tgagctggct ccagcatgga agggtttttt tttgtccgaa atcaaaatat
    21601 aaaattcagt gataatgtga actatcatta tcgctttaac attaatagtt gtgcaaaatt
    21661 tcttgctttt tgggactatt tcagtggtgc tctagtagag catagccatg ctgaaaaatg
    21721 tattcatttc tatcatgaga atgatcttcg ggatagttgt aataccgaat cgatgcttga
    21781 taagttaatg ttaagattca tcttttcatc tgaccagaat gtaagtaatg cactggctat
    21841 gatacgaatg actgagtcat atcatttggt tctttatctt ctgagaacta ttgagaaaga
    21901 aaaggaggta agaataaaga gtctgacaga gcattatggt gtgtcggagg cttattttag
    21961 atctttatgt agaaaagctt taggggcaaa agtcaaagaa caactgaaca cttggcggtt
    22021 agtaaatggt ttattagatg tatttttgca caatcaaaca atcacttctg ctgccatgaa
    22081 taatgggtat gcatcaacat cccatttttc aaatgagata aaaacacgac taggcttttc
    22141 tgcaagagaa ttatcgaata ttactttttt agtgaaaaaa ataaatgaaa aaatttaata
    22201 ttaaatcttt gactctcttg attgtattgt taccattgat agttaatgct aataatattg
    22261 actctcattt attagaacaa aatgacatag cgaaatatgt tgctcaaagt gatacggtag
    22321 gatctttttt tgaacgtttt tctgcgttat taaattatcc tattgttgtt agcaaacaag
    22381 ctgcaaaaaa gagaatatct ggggagtttg atttatctaa tccagaagaa atgcttgaaa
    22441 aaatgacatt acttgtggga ctcatatggt ataaagatgg aaatgcatta tacatatatg
    22501 actctggaga attaataagc aaagttattt tgctggagaa tatatcactt aattatctta
    22561 ttcagtatct gaaggatgct aacctttatg atcacagata tcctataaga ggaaacatta
    22621 gtgataaaac attctatata tctggtcctc cggcattggt tgaactggta gctaatacag
    22681 caactttgct tgataagcag gtttcttcta ttgggacaga taaagttaat tttggggtta
    22741 taaaattaaa aaatacattt gttagtgaca gaacttataa tatgagaggg gaggatattg
    22801 tcatcccagg ggtggctact gttgtagaac gtttattaaa taatggtaag gctctgagta
    22861 acagacaagc caaaaatgat ccgatgccac cctttaatat tacacagaaa gtatctgagg
    22921 atagtaatga cttttcattt agttcagtta ccaatagttc tatattagag gatgtatcac
    22981 ttattgcata tccagaaacc aattctatat tagtcaaagg taatgatcag caaatacaga
    23041 ttattcgcga tattatcact cagttagatg tagccaaacg acatatagaa ttatcattat
    23101 ggataatcga tattgataaa tctgaattaa ataaccttgg cgttaattgg caaggaacgg
    23161 cttcatttgg tgattcgttt ggtgcatcat ttaatatgag ctcatcagca tcgataagca
    23221 ctttggacgg caataagttt attgcttcag ttatggcatt aaatcagaaa aagaaagcaa
    23281 atgtagtttc aaggccggtg attttgacac aagagaatat tccggctata tttgataata
    23341 acaggacttt ttatgtgtca ttagttggtg agcgcaattc ttcgttggag catgttacgt
    23401 atggaacatt gataaatgtt atacctcgct tttcatctca gggacaaatt gagatgtctt
    23461 taacgataga ggatggaaca ggaaatagtc agtcaaatta caattataat aatgaaaata
    23521 catctgtcct tccagaagtt ggaaggacca aaatcagtac aatagcgaga gttccacaag
    23581 gcaaaagttt gttgataggt ggttatacgc atgaaacgaa ctctaacgaa atagtatcta
    23641 ttccttttct ctcctctatt cctgtaatcg gtaatgtgtt caagtataaa acaaggaata
    23701 tcagtaatat tgtaagggtt tttctgattc aaccccgtga gataaaggag tcaagttatt
    23761 ataatactgc agaatataag agcctcatat ctgaaagaga aatacaaaag actacacaaa
    23821 taatcccatc tgaaactact ttgctggaag acgaaaaatc attggtttca tacttaaatt
    23881 actaactata aagtaggtga tgtatgcttg atgttaaaaa tacaggagtt tttagctctg
    23941 cattcattga taagttgaat gcaatgacaa attcagatga tggagatgag actgctgatg
    24001 cagagcttga ttctggcttg gctaatagca agtatattga ctcatctgat gagatggctt
    24061 ccgctctttc gtcatttata aacagaagag accttgagaa actgaaagga acaaatagtg
    24121 atagtcagga acgtatttta gatggggaag aagatgaaat taatcacaag atttttgatt
    24181 taaagagaac tttaaaagat aaccttcctc tagatcagga ttttatagac agactaaaga
    24241 gatattttaa agatccaagt gatcaagtct tagcattaag ggaacttttg aatgaaaaag
    24301 atcttactgc tgaacaagtc gaatcattaa ctaaaattat taatgagata atatcaggta
    24361 gtgaaaaaag tgttaatgct ggaataaatt cagctataca ggctaaatta tttggcaaca
    24421 aaatgaaact tgaaccacag cttttgcgtg catgttatcg tggttttatc atggggaaca
    24481 tatcaacaac agatcagtat atagaatggc ttggtaattt tggttttaat cacagacata
    24541 caattgtgaa ttttgtagag cagtcactga ttgtagacat ggattctgag aaaccgagct
    24601 gtaatgctta tgagtttggt tttgtgttat ctaaattaat tgcaattaag atgattagaa
    24661 cttcagatgt aatttttatg aagaaactgg aatcctcaag cttgctaaaa gatggcagtt
    24721 taagtgcaga gcagctattg ctaactttat tatatatttt tcaatatcca agtgaaagtg
    24781 agcaaattct tacttctgtt atagacgtat cacgagccag tcatgaggat tctgtagtgt
    24841 atcaaacata tctatcttct gttaatgaaa gtcctcatga tatatttaaa agtgaaagtg
    24901 aaagagaaat tgcgatcaat attctacgag agcttgtcac aagtgcatac aagaaagagc
    24961 tttctagata acaggagatg aaagtgatcc agtcttttct taagcaagta agtactaagc
    25021 ctgaattaat tatattagtg cttatggtta tgatcattgc tatgttgatc ataccattac
    25081 ctacatacct tgttgatttt ttaatagggc ttaatattgt acttgctatt ctcgttttta
    25141 tgggatcatt ttatattgaa agaatattga gtttttctac attcccttcg gttttgttga
    25201 taacgacatt atttcgtctc gctttatcaa taagtactag tcgactaatt ttagttgatg
    25261 cggatgcggg taaaattatt actacatttg gccagtttgt cattggtgat agtctggctg
    25321 taggttttgt tattttttca atagtaactg ttgtgcagtt cattgttata acaaaaggat
    25381 ctgaaagggt tgcggaggta gctgctcgct tttctcttga cggtatgccg gggaaacaaa
    25441 tgagcattga tgcggattta aaggctggaa ttattgatgc cgcaggagct aaagaaagac
    25501 gcagtattct ggaacgtgag agtcaattgt atggttcatt tgatggagca atgaagttca
    25561 ttaaaggtga tgcaattgct ggcatcatta ttatttttgt caatttaata ggaggtattt
    25621 ctgttggtat gagtcaacat ggaatgtctc tatctggtgc attatctact tataccatac
    25681 taactattgg tgacggattg gtctctcaga ttcctgctct gttaatttct ataagtgcag
    25741 gatttatcgt tacaagagtt aatggtgata gcgataatat gggacgtaac ataatgtccc
    25801 agatatttgg gaatcctttt gttctcattg ttacatcagc tcttgccttg gcgataggga
    25861 tgttgccagg ttttccgttt tttgtttttt tcctgatagc agttactttg acggctttat
    25921 tctattataa aaaggtcgta gaaaaagaaa aaagtctgtc tgagtctgat tctagtggct
    25981 atactggtac atttgatatt gataatagcc atgactcatc tttggcaatg atagaaaatc
    26041 tggatgctat tagttcagaa accgttcctt taattttatt atttgccgaa aataagataa
    26101 gtgcaaatga tatggaaggt cttattgaaa ggataagaag tcagttcttt attgattatg
    26161 gtgtcaggct tccaactatt ttatatagaa caagcaatga gcttaaggtc gatgatattg
    26221 ttttgttaat aaatgaggtg cgtgccgata gttttaatat atactttgac aaagtgtgta
    26281 ttacagatga aaatggagat atagatgctc taggtattcc tgttgtttca acttcatata
    26341 atgaacgtgt tatttcttgg gtagacgttt catatacaga aaatctaact aatattgatg
    26401 ctaaaattaa aagtgctcaa gatgaatttt atcaccagtt gtcacaagct ttattaaaca
    26461 acataaatga gatttttggt atacaagaaa caaaaaatat gttagatcag tttgaaaatc
    26521 ggtatcctga tctattaaag gaagtcttcc gacatgtgac tatacagaga atttctgagg
    26581 tattacaaag attgcttgga gaaaatattt ctgttcgcaa tttaaaactt attatggagt
    26641 ctttggcgct ttgggctccc agagaaaaag atgtcataac attagttgaa catgtccgtg
    26701 catcactttc taggtatatt tgtagtaaaa tagctgtttc tggtgagatt aaagttgtga
    26761 tgctttccgg atatattgag gatgcaataa gaaaagggat aaggcaaacc tctggtggct
    26821 ctttcttgaa tatggatata gaggtttcgg atgaggtaat ggaaacttta gcacatgctt
    26881 tgagagaatt gagaaatgca aaaaaaaatt tcgttctttt ggtatcagta gatatacgta
    26941 ggtttgttaa aagacttata gataacagat ttaagagtat actcgttata tcgtatgctg
    27001 agattgatga agcatatacc attaatgtat taaagactat ttagtgaggt ttaaatatga
    27061 gtaacattaa tttagttcaa ttagttagag atagtctttt cacgattggt tgtcctccat
    27121 caattattac agatttagac agccattcag cgattaccat ttctttggat agtatgcctg
    27181 ctattaatat agctcttgtc aatgagcagg ttatgttatg ggctaatttc gatgcaccaa
    27241 gtgatgtaaa acttcagtca tctgcttata acatattaaa tttaatgtta atgaacttta
    27301 gttatagcat taatgagctg gtagagcttc atcgttctga tgagtactta cagttaagag
    27361 ttgttattaa agatgactac gttcacgatg ggattgtttt tgcagagatt ctgcatgagt
    27421 tttatcagag gatggaaatc ttaaatgagg tcttataatc aatgagctat ataaaattgc
    27481 tcactcaatt atcttttcct aatagaatct cggggccaat cttggaaaca agtcttagcg
    27541 atgtttcgat tggtgagatt tgtaacattc aggctggaat tgaaagtaat gaaattattg
    27601 caagagctca ggttgtagga tttcatgatg aaaaaacaat attaagcttg attggaaatt
    27661 ctcgtggact ttcaaggcaa acgttgatta agcccactgc ccagtttctt catacgcaag
    27721 tcggccgtgg attattggga gcagtagtca atcctttagg ggaggttact gataagtttg
    27781 ctgttacgga taacagtgaa attctttatc gacctgtaga taatgctcct ccgctatata
    27841 gtgaaagggc tgcaattgag aagccttttt taacaggtgt taaggttatt gattctttac
    27901 tcacgtgtgg tgaaggacag cgaatgggga tttttgcgtc agctggttgt ggcaaaactt
    27961 ttctcatgaa tatgctcatt gaacatagtg gtgctgatat atatgttatt gggttaattg
    28021 gtgagcgagg tcgagaggtt acagaaacgg ttgattattt gaagaactct gagaaaaaaa
    28081 gcaggtgtgt tttagtatat gcaacttcgg attactcttc ggttgatcgt tgtaatgctg
    28141 catatatagc cactgctata gccgaatttt ttaggactga aggacataaa gtagcgcttt
    28201 ttattgattc attaacaagg tatgccagag cattacgtga tgtggcctta gccgctggag
    28261 aatcacctgc cagaagaggc tatccggttt cggtttttga tagcttaccc agacttcttg
    28321 aaaggccagg aaagttaaag gcaggtggct ctattactgc attttacact gttcttttgg
    28381 aggatgatga ttttgctgat ccattagctg aagaggtaag atccatttta gatggacata
    28441 tatatttgag cagaaatcta gcccaaaaag gacaatttcc tgcaattgat tccttaaaaa
    28501 gtataagcag ggtatttaca caggttgttg atgaaaaaca tcgtattatg gccactgcat
    28561 ttcgggagct actttctgaa atagaagagc taaggacaat tattgacttt ggtgaataca
    28621 aaccggggga gaatgcctct caggataaaa tctacaacaa aatatctgtt gttgagagtt
    28681 ttctgaagca agattatcga ctcggtttta cttatgagca gacaatggag cttcttggtg
    28741 aaacaattag ataaggtttt aaaaatcaaa gataaatatc aacggtctgt taaattaatt
    28801 gaagcacaca tattaacact aaaaaaaaaa ctctttgtat cgagatgtgg aggcattaga
    28861 taaaaggatt atatacttcc tgcaactgga gaatgacttg gaacctgtag gcgctcagag
    28921 tgttagtcag cttttcaata caagacgtaa gatcgctata gtaaaaaagc atattatcca
    28981 atatcagtct gaacgaattc ttctaaaggg aagaattgaa gaaattcaaa aagatattga
    29041 tgaggcaaat gcttctaaaa gaaaactgct tcataaagag agtaaaatat gtaaacggat
    29101 tggcttgatt aaaagaaata actttgccaa acagttgata cttgatgagt tatcacaaga
    29161 ggatatgaag tatggcatta gataatataa acctaaattt tagtagtgac aaacagatag
    29221 aaaaatgtga gaaactatct tcaatagata atattgatag tctcgttttg aagaagaaga
    29281 ggaaggttga aattcctgag tactctttaa ttgcatctaa ctattttact attgataagc
    29341 actttgaaca taagcatgat aaaggagaaa tttatagtgg cattaaaaat gcgttcgaac
    29401 ttagaaacga acgagcgaca tattctgata ttccagaatc aatggccatt aaagaaaata
    29461 ttttgatacc agatcaagat atcaaagcaa gggaaaaaat aaatatcggc gatatgaggg
    29521 ggatcttttc atataataag agtggaaatg cagacaagaa cttcgaaaga agtcatactt
    29581 cttctgtaaa ccctgataat ctgctagaat ctgataatag aaatggtcaa attggtttaa
    29641 aaaatcatag cttgtctatt gataagaata ttgctgacat catttcttta ctaaatggaa
    29701 gtgttgctaa atcatttgag ctgcctgtaa tgaataaaaa tactgcagac ataaccccat
    29761 ccatgtcatt gcaagaaaaa tcaatagttg aaaatgataa aaatgttttt caaaaaaata
    29821 gtgaaatgac ttaccacttt aaacagtggg gggctggaca ttctgttagt atttcagtgg
    29881 agtctggttc ttttgttcta aaaccgtcag atcaatttgt aggaaataaa cttgacttaa
    29941 ttttgaaaca agatgctgag ggtaattaca gatttgatag cagtcaacat aataagggga
    30001 ataaaaataa tagtacagga tataatgaac agagtgaaga agaatgctaa gaattaaaca
    30061 ttttgacgct aacgaaaaac tacagatttt atatgcaaag caactctgcg agcgtttttc
    30121 gattcagaca ttcaaaaata aatttacagg cagtgaaagt ttagtcactc ttacttccgt
    30181 gtgtggggat tgggtaattc gtattgatac attatctttt ttgaaaaaaa aatacgaggt
    30241 attttcagga ttttctacac aagaatcttt actgcattta tcaaaatgtg tctttataga
    30301 gtcgtcatct gtattttcga ttccagaact gtctgataag attactttcc ggatcacgaa
    30361 tgaaatccag tatgcaacta ctgggagtca tttatgctgt ttttcctctt ctttaggtat
    30421 tatttatttt gacaagatgc cggtattacg taatcaagtt tctcttgact tattgcatca
    30481 tcttttagag ttttgcttag gttcatctaa tgtaaggctg gctactttaa aaagaattcg
    30541 tactggtgat ataatcatag ttcagaaact ttataattta ttattgtgta atcaagttat
    30601 tattggggat tatattgtga atgataataa tgaggcaaaa attaatctgt cagaaagtaa
    30661 tggtgagtca gatcacacag aagtttcttt ggcattattc aattatgatg atatcaatgt
    30721 aaaagtggac tttatccttt tagaaaaaaa gatgacaatc aatgaactaa aaatgtatgt
    30781 agaaaacgaa ttattcaagt ttcccgatga catagttaaa catgtaaata ttaaagtaaa
    30841 tggttctttg gttgggcatg gggaacttgt ttctattgag gatggttatg gtatcgagat
    30901 tagttcttgg atggtaaagg agtaatctca tgctgagtga tatgtccctc atcgctacac
    30961 tttctttttt tacgctatta ccatttttag ttgctgctgg tacttgttat ataaagtttt
    31021 ctattgtttt tgtaatggta cgaaatgcac ttggtttgca gcaggttcca tcaaatatga
    31081 ctctcaatgg tattgcattg attatggctt tgttcgtgat gaaacccatt attgaagctg
    31141 gttatgaaaa ttatctaaat ggcccgcaaa aatttgatac aatatcagat attgtccgtt
    31201 tttctgattc tggcttgatt gaatacaagc aatacttgaa aaagcataca gatcttgagt
    31261 tggcacgttt tttccaaagg tctgaagaag aaaatgctga tttaaagagt gcagagaata
    31321 atgattattc attgttttcc ctcttgcctg catatgcgct aagtgaaatt aaagatgcgt
    31381 tcaaaatagg tttttattta tatttgccat ttattgttgt tgatcttgtt atttcaagca
    31441 ttttacttgc acttggtatg atgatgatga gtccaatcac aatatcagta ccaataaaac
    31501 ttgtattatt tgttgcgctt gatgggtggg ggatcctgtc taaggccttg attgaacaat
    31561 atatcaatgt tcctgcttag atgatggagg taatgtgtct gatatagttt atatgggtaa
    31621 taaggctctt tatttaatcc ttatcttttc cttatggcca gtaggtatag ctacggttat
    31681 tggattaact attggtttat tacagacagt gactcaactt caagagcaga cacttccttt
    31741 tggtataaag cttataggtg tctcaatatc tttgctactt ctttctggat ggtatggtga
    31801 ggttttattg tctttttgtc atgaaataat gtttttaatt aagagtgggg tttgatggac
    31861 atttcaagct ggttcgaaag tattcatgtg tttttaatac tcctgaacgg cgtttttttt
    31921 agattggctc cattattttt ctttcttcca tttttaaata acggtataat ttctccatct
    31981 attagaatac ctgtgatttt tcttgttgca tcaggattaa ttacttctgg taaggtagac
    32041 ataggttctt ctgtttttga acatgtttat ttccttatgt tcaaggaaat aattgttggc
    32101 ctccttctct ctttttgctt gtctcttccc ttttggatat ttcatgctgt tggtagtatt
    32161 attgacaacc agcgtggggc aacgcttagt agttcaattg atcctgccaa tggtgttgat
    32221 acgtctgaat tggcaaaatt tttcaatctt ttttctgcag ttgtatttct atacagtggt
    32281 ggaatggtct ttattttaga atccatacaa ttgtcttata atatatgccc gttattttct
    32341 caatgttctt tccgtgtctc aaatatctta acatttctga ctttattggc aagtcaggct
    32401 gttattttag ccagtcctgt tatgatagta ttgttactat cagaagtatt acttggtgta
    32461 ttatcgagat ttgctccgca gatgaatgct ttttccgtat cattaactat taaaagttta
    32521 cttgcaatat ttattatttt catctgttct tctactattt acttttctaa agttcaattt
    32581 ttcctcggtg aacataaatt tttcacaaat ctatttgtta gataaaatat tatggcaaat
    32641 aaaacagaaa agccgacacc taaaaaacta aaggatgccg caaaaaaagg acagtcattt
    32701 aaatttaagg atttaacgac agttgttatt attctggtag ggacatttac tataatatca
    32761 ttcttttcct taagtgatgt aatgctctta tacagatatg taataattaa tgacttcgaa
    32821 attaatgagg gtaaatactt ttttgcagtg gttattgtct tttttaagat aattggcttc
    32881 ccactttttt tctgtgttct ttcggctgtg ttgccaacat tggttcaaac aaagtttgtt
    32941 cttgcgacta aagctatcaa gattgatttt tcagtattaa accctgttaa agggttaaaa
    33001 aaaatattta gtataaagac aataaaagaa tttttcaaaa gcattctgct tcttattatt
    33061 ctagcattaa caacctattt cttttggatt aatgaccgaa aaataatttt ttctcaggtg
    33121 ttttctagtg ttgatggctt atatcttatt tggggggggc tatttaagga tataatatta
    33181 tttttcttag cattttctat ttttgttatt attcttgact ttgtgattga gttcatttta
    33241 tacatgaaag atatgatgat ggataaacag gagataaaaa gagaatatat agagcaagag
    33301 ggacactttg agacaaagtc gagaaggcgt gagttgcata tcgagattct ttcagagcag
    33361 actaaatctg atatacgtaa ttcaaaatta gtggtaatga acccgactca tattgcaatt
    33421 ggtatttatt ttaatccaga aatagcgcct gcacctttta tttctctcat tgaaactaac
    33481 cagtgtgcct tggctgtcag aaaatatgca aatgaagttg gtataccaac tgtgcgtgat
    33541 gtgaaattag ctagaaaact atataaaaca catacaaaat atagttttgt tgattttgaa
    33601 cacttggatg aagtcctacg tcttattgtt tggcttgagc aggttgaaaa cactcattaa
    33661 taagtaaagg agatgtgtta tatgggagtt aatttctgta ataaaatagg tattgatcag
    33721 tctgaatttg aaatagaatc ttccatcatt aactccattg ctaatgaggt attgaaccca
    33781 atatctttcc ttagcaacaa ggatataata aatgttttac tcagaaagat ttcatctgaa
    33841 tgtgacctgg ttagaaaaga catttatcgt tgtgctcttg agttggtcgt tgaaaaaaca
    33901 cctgatgatt tataatcatt cataataact cttttgttga tataaatatt tttgttgaag
    33961 atatttatgt agtcacagga tcaattgtag aaaattgtta tgtttttgtt aggaactgaa
    34021 ctaaataata tatgataaga cagcaaaaac gactgaccat aatccttctt ttattaggag
    34081 ttgataagag agactattca tcctgtaatg ttaaaactct gttatatagt attcgcgatt
    34141 atgcaaagtc tgtaaatgat catgagatac taaccgagag taatcgttta ttatctcatt
    34201 gcatttcaga tagtaacgga gctttcttta aaagtagtaa atacgttccc cttaagtact
    34261 tacgtaaaag gagaatagca cgaaagatac caaatgatta gcactttgct agtaagcttg
    34321 ctggtaaact atattgaccg ttgggtttgt tttgagactg taatttaaat tgtgtaattg
    34381 cctgtttttg atatgtttac tccaacaacg gagacaggta aattatggac gaaaagaagc
    34441 ttaaagccct tgcggctgaa ctggctaaag gcctcaaaac tgaggccgac ctcaatcagt
    34501 tttcccgtat gctgacgaaa tatcccggct cttaaaacga tagtggctca ggctttcatt
    34561 tttcagcgtt ccccagaaga tt
//
LOCUS       DAJTOR010000025        34294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTOR010000025 DAJTOR010000000
VERSION     DAJTOR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.025"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..467
                     /locus_tag="OO249_001062"
                     /pseudo
     CDS             1..467
                     /locus_tag="OO249_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            570..1072
                     /locus_tag="OO249_001063"
                     /pseudo
     CDS             570..1072
                     /locus_tag="OO249_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1139..2596)
                     /gene="nuoN"
                     /locus_tag="OO249_001064"
     CDS             complement(1139..2596)
                     /gene="nuoN"
                     /locus_tag="OO249_001064"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5635436.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQSLISIVRLAMPLM"
     gene            complement(2603..4132)
                     /gene="nuoM"
                     /locus_tag="OO249_001065"
     CDS             complement(2603..4132)
                     /gene="nuoM"
                     /locus_tag="OO249_001065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5635437.1"
                     /translation="MLLPWLILIPFIGGFLCWQTEHFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4296..6137)
                     /gene="nuoL"
                     /locus_tag="OO249_001066"
     CDS             complement(4296..6137)
                     /gene="nuoL"
                     /locus_tag="OO249_001066"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5635438.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6134..6436)
                     /gene="nuoK"
                     /locus_tag="OO249_001067"
     CDS             complement(6134..6436)
                     /gene="nuoK"
                     /locus_tag="OO249_001067"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5635439.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6433..6987)
                     /gene="nuoJ"
                     /locus_tag="OO249_001068"
     CDS             complement(6433..6987)
                     /gene="nuoJ"
                     /locus_tag="OO249_001068"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5635440.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6999..7541)
                     /gene="nuoI"
                     /locus_tag="OO249_001069"
     CDS             complement(6999..7541)
                     /gene="nuoI"
                     /locus_tag="OO249_001069"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5635441.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7556..8533)
                     /gene="nuoH"
                     /locus_tag="OO249_001070"
     CDS             complement(7556..8533)
                     /gene="nuoH"
                     /locus_tag="OO249_001070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5635442.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8530..11256)
                     /gene="nuoG"
                     /locus_tag="OO249_001071"
     CDS             complement(8530..11256)
                     /gene="nuoG"
                     /locus_tag="OO249_001071"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5635443.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11309..12646)
                     /gene="nuoF"
                     /locus_tag="OO249_001072"
     CDS             complement(11309..12646)
                     /gene="nuoF"
                     /locus_tag="OO249_001072"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5635444.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12643..13143)
                     /gene="nuoE"
                     /locus_tag="OO249_001073"
     CDS             complement(12643..13143)
                     /gene="nuoE"
                     /locus_tag="OO249_001073"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5635445.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13146..14948)
                     /gene="nuoC"
                     /locus_tag="OO249_001074"
     CDS             complement(13146..14948)
                     /gene="nuoC"
                     /locus_tag="OO249_001074"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5635446.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15042..15704)
                     /gene="nuoB"
                     /locus_tag="OO249_001075"
     CDS             complement(15042..15704)
                     /gene="nuoB"
                     /locus_tag="OO249_001075"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5635447.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15720..16163)
                     /gene="nuoA"
                     /locus_tag="OO249_001076"
     CDS             complement(15720..16163)
                     /gene="nuoA"
                     /locus_tag="OO249_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5635448.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16794..17732)
                     /gene="lrhA"
                     /locus_tag="OO249_001077"
     CDS             complement(16794..17732)
                     /gene="lrhA"
                     /locus_tag="OO249_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5635449.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18651..19868
                     /gene="alaA"
                     /locus_tag="OO249_001078"
     CDS             18651..19868
                     /gene="alaA"
                     /locus_tag="OO249_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5635450.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19952..20551
                     /gene="yfbR"
                     /locus_tag="OO249_001079"
     CDS             19952..20551
                     /gene="yfbR"
                     /locus_tag="OO249_001079"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5635451.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVLTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20610..22442)
                     /locus_tag="OO249_001080"
     CDS             complement(20610..22442)
                     /locus_tag="OO249_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5635452.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22529..23179)
                     /gene="hxpA"
                     /locus_tag="OO249_001081"
     CDS             complement(22529..23179)
                     /gene="hxpA"
                     /locus_tag="OO249_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5635453.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPKVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQLNGDVIIQ"
     gene            complement(23190..23684)
                     /locus_tag="OO249_001082"
     CDS             complement(23190..23684)
                     /locus_tag="OO249_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5635454.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACLRQYHLSANEINQ
                     IINA"
     gene            complement(23768..24223)
                     /locus_tag="OO249_001083"
     CDS             complement(23768..24223)
                     /locus_tag="OO249_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5635455.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24561..25763
                     /gene="ackA"
                     /locus_tag="OO249_001084"
     CDS             24561..25763
                     /gene="ackA"
                     /locus_tag="OO249_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5635456.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25838..27982
                     /gene="pta"
                     /locus_tag="OO249_001085"
     CDS             25838..27982
                     /gene="pta"
                     /locus_tag="OO249_001085"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5635457.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNQEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28172..29692
                     /gene="yfcC"
                     /locus_tag="OO249_001086"
     CDS             28172..29692
                     /gene="yfcC"
                     /locus_tag="OO249_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5635458.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVSRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29725..30267)
                     /gene="yfcD"
                     /locus_tag="OO249_001087"
     CDS             complement(29725..30267)
                     /gene="yfcD"
                     /locus_tag="OO249_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5635459.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30325..30876)
                     /gene="yfcE"
                     /locus_tag="OO249_001088"
     CDS             complement(30325..30876)
                     /gene="yfcE"
                     /locus_tag="OO249_001088"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5635460.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30932..31564)
                     /gene="yfcF"
                     /locus_tag="OO249_001089"
     CDS             complement(30932..31564)
                     /gene="yfcF"
                     /locus_tag="OO249_001089"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5635461.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASVAMAEHLLALGQPNLFGEW
                     CIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31700..32347
                     /gene="yfcG"
                     /locus_tag="OO249_001090"
     CDS             31700..32347
                     /gene="yfcG"
                     /locus_tag="OO249_001090"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5635462.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGELGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32404..32766
                     /gene="folX"
                     /locus_tag="OO249_001091"
     CDS             32404..32766
                     /gene="folX"
                     /locus_tag="OO249_001091"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5635463.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32787..33680
                     /locus_tag="OO249_001092"
     CDS             32787..33680
                     /locus_tag="OO249_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5635464.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33728..>34294)
                     /gene="rpnB"
                     /locus_tag="OO249_001093"
     CDS             complement(33728..>34294)
                     /gene="rpnB"
                     /locus_tag="OO249_001093"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5635465.1"
                     /translation="AGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSS
                     AFPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLK
                     ALFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQ
                     EMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8576 a         9342 c         8614 g         7762 t
ORIGIN      
        1 cgacaatatt gataatctga ataggtccgt agagtatata aaagagttct ttgtcagtag
       61 cggtgccaga gttaccgcgc agggcgtccc cgattggcta ttacgactct gcgcctggta
      121 gccaggatta cctttacccg caatgtcctg gctttatccc gatcgggggg actttattgc
      181 ctggtcggca gaatacagga tatcaacgcc gctcgtcagg taaaaacggc attgttatca
      241 tcccgggatt tatctgttta ttctatgaat gccccatggt ttattcccgg tattgatttc
      301 tctgaccacc tgaattattg gcaacacgat attcccgccg taatgattac tgacaccgct
      361 ttttatcgta ataaacaata ccacttgccc ggtgatacag cagacagatt gaattatcag
      421 aaaatggctc agatggtgtt ataaccttgt tacacaacag taaataataa aaatttctcg
      481 aggcgggtcc agaaacagcg acatgtacat tttattttcc ctcgtctgtt ttaagatgcg
      541 aaacataaaa ttcaatggag gaaaaataaa tgggaatgat tggttatttt gcagaaattg
      601 actcagaaaa gattaaccaa tctgcttgag tctactgaaa aacctttgat ggataatata
      661 catgacacgc tttcaggatt acgccgactg gatattgata aacgctggga ttttttacac
      721 tttgggctga caggcacccc tgcttttgat cccgcgaaga atgatccgct gagtcgtgcc
      781 gtgcttggtg aacacagcct cgaagatggc atagatggct tcctgggatt gacctggaat
      841 caggaattag ccgcgacaat cgaccgactg gaatcactgg atcgaagtaa gctgcgtaaa
      901 cagttttcca ttaagcgact atgaaatgga gatttatccg ggagtgacct tttcagaaga
      961 gttagagggg caactctttg cctcgatcat gcttgatatg gaaaagctga ttagcgcgta
     1021 tcgacgtatg ctgcgacaag ggaatcatgc gctgacggtt attgttgggt gatggtgtca
     1081 gagcgccaaa aaaaaccgcc gaacatgtcg gcggttttct gacttacaaa ctaacagatt
     1141 acatcagcgg cattgctaac cgcacaatac taatcagcga ttgtggccat acacccagca
     1201 ccagtaccaa cagtgcagaa atcagcacca cgataccgcc cgcgctgtac tgccagtttg
     1261 acggtgcatc gcgacccggt tgttccggtg cgtgcaggta caggctcacc gccacgcgca
     1321 ggtagtagta gaggccgatt gccgaaccga caaccacggc acccaccagc caccacaagt
     1381 gtgcctggac accgaccgcc agcacgtaga acttaccgat aaagcccagc gtcatcggga
     1441 taccggccag agacagcatc atcaccgtca tcactgccgc gaggatcgga cgatgccaga
     1501 acagaccgcg gtaagagaac agggaatcag catccgggcc acgatacggg ctggacatca
     1561 ggctgaccac gccgaacgcg ccgaggctgc tgaacagata accggccagg taaaccccta
     1621 ccgcttccat cgacatctcg ccggtttgca gcgcaatcag cgccaccagc agatagccga
     1681 ggtgagagat agatgagtaa ccgagcagac gtttgatgtt ggtctggctc aacgccatca
     1741 ggttaccgaa gatgatggag gcaaaggcga taatcgccag tactacgcga atcgcttcgc
     1801 tgtcacccac cggtgcatag aggaacagac gcatcaccac accgaagata gcgattttgc
     1861 tcgccgtcgc caggaaagtg gaaaccggcg caggcgcgcc ttggtatacg tctggcgtcc
     1921 acaggtggaa cggcaccaga gagagtttga agccgaggcc aacaatcatc atgccgaaac
     1981 ctgccagcaa cagcggctcg ttgagcatac cgtcaccgag gtttttaccc agcgcgacaa
     2041 acgacaggtc gccagactgc gcatacacca gcgccatacc aaacagcagg aaagaagacg
     2101 ctgcggcaga aaggatggtg tatttgatac tggcttccag cgaacgtttc tggcggaaag
     2161 cgtaaccgac caggccaaac agcggcaaag agatcagttc aataccgagg aacagagacg
     2221 ccagatggtt tgcattcgcc agcaggatcc cgcccagcgc ggcaattaac accaacaggt
     2281 agaactcatc cttgttgtcg ttatagcctt caagccacgg gtaggcgaaa gtacaggtgg
     2341 cgaggctcgc caacaatacc agcccggtgt aaagcatggc gaaaccatca acgcgcatca
     2401 gcggcgtaac gtccatagcg cccgcctggc caacaaacca gagcgaaacc agcgccgcgt
     2461 taagcccaat aaccgagagc gtagcgttga ggaaatgatt gcgtcgccac gcaatggaga
     2521 gcatcacaac caccaccgtt aagccgacga tcagcaacgg tagcagtgcg atcaggtttt
     2581 gtggagttat tgtcatggcg atttacggcc ttgtagtagt aacggaatta acaaaccact
     2641 gctggatatt gccaatcgcg gagtgcgagg tatccagaat cggctgcgga tagaagccca
     2701 gcagtaccag cagcaccacc agcaacagga tcataaacag ctcacgcagc gacatccctg
     2761 gcagttcctg gctggcaatc tggcttttcg ctttaccgaa gtaagcgcga tgcaacatcg
     2821 ccagcgaata aacagatgca aagaccagcc caaaggtaga gataacggta atcaccggga
     2881 caacctggaa gctgccgaac agaatcataa attcgccgac gaagttaccg gtgccaggca
     2941 tcccaagcgt tgccaccgca aagaacagcg acaatgctgg cagccatttc atcttgctcc
     3001 acagaccgcc catcatgcgc atgtcgcggg tatggatacg ttcataaagc tgaccacaaa
     3061 gaataaacag acccgccgcc gacaaaccgt gcgcaatcat ctggattacc gcgccctggt
     3121 aggccaactg gctgccggtg tagatagcaa tcagcacgaa gcccatgtgg gaaaccgagg
     3181 tgtaggcgat cagacgtttg atatcggtct gggcgaaggc catccacgca ccgtagaaga
     3241 tgccgataac acccagccac atagcaattg gcgcgaactc tgccgacgcg ttcgggaaca
     3301 gcggcaggga gaaacgcagc aaaccgtaag cggcagtttt cagcaagatc cccgcgagat
     3361 caacggaacc ggcggtcgga gcctgggagt gcgcatccgg cagccagcca tgcagcggaa
     3421 ccaccggcat tttgactgcg aaggcgatga agaagcccag cattaacagg tattccacac
     3481 cactggacat tggcgtattc agcagctctt catagttgaa ggtccagacg ccggtcgcat
     3541 tgtagtgaac aaaaaccagc gccaggatgg caatcaacat caccaggcca ctcgcctggg
     3601 tgtaaatgaa gaacttggtt gccgccgtga tacgcgtttt accgtcagag gctttatgcc
     3661 cccacagtgc gatcaggaag tacatcggca ccagcatcat ttcccagaag aagaagaaca
     3721 ggaacatgtc gatggcaagg aacacgccga taacgccgcc caggatccac atcaggttga
     3781 ggtggaagaa gccctgatat ttttcgatct ctttccacga acagagtacc gccagcacac
     3841 cgagcagacc ggtcagcacg accatcagca gcgacagacc gtcaatggcg agatgaatag
     3901 agataccaaa acgcgggatc cacggcatgt cgaattcaga ctgccactgc ggaattccgg
     3961 cggattgcgt cagtgaataa ccgccctgca accacagttg cagcgacagc gccagcgtca
     4021 atcccatggt gatcagcgcg atccagcgtg gcaccttgac gtcaaagtgt tcggtctgcc
     4081 agcacaggaa gccgccaata aagggaatta atattagcca gggtagtaac atggcgatct
     4141 ttattccttg taaaagtccc gtcaggaccg gattttcaac gaattctcac gacagaaaat
     4201 tctcttcgga ctgggggtag tgtcggatgc gccgcgaagg cagcttatcc gacctacgtt
     4261 cttatatgcc caggagcaat cccacaatcc ttaactcaac gcagtaccat caacagtgcc
     4321 agcaccacga ccgcaccgat gctcatggat gccacatacc agcgcagata accgttctcg
     4381 cttaacagca ggcctttacc tgcaaagcgg gaaagaaccg ccggaatgtt catcattgag
     4441 ttcagcggat cgcgtttcag caaccaggca atacccagaa acggcttgac gaacactttg
     4501 tcgtacagcc agtcaaagcc ccaggcgttg taccaccagg tgcccagcag acggcccggc
     4561 gcactgttgg caatggaggt caccagagta cgtttaccct gccacagcca ggctgccagc
     4621 agaatgccga ccaccgcgac cacgccagag gtaatttcca gggtcaacat gctgccgtgc
     4681 gccagttccg tcgtttgcgg aagcacgctc tgcagcggcg gtacaatcag tgcgccaacg
     4741 aaggtggaaa ggatcagcag cacaatcagc ggcaggctgt gagttacccc tttcacggcg
     4801 tgagcgtgaa tttgttcttt tccgtggaag acgatgaaaa tcatacggaa ggtgtagagc
     4861 gaggtcataa acgcaccgac cagacctgcc accatcagat tgatatgacc attcgccatc
     4921 gcacccgcga ggatctcatc cttactgaag aagcccgcag tgaccagcgg tagtgccgac
     4981 agtgctgcgc cgcccaccag gaagcagaaa taaaccagcg gaatagattt acgcagaccg
     5041 cccatcttga agatgttctg ttcgtgatgg caggccagaa tgacggaacc ggatgccagg
     5101 aacagcagcg ctttaaagaa cgcgtgagtc atcaagtgga aaatcgccgc atcccatgcc
     5161 tgcacgccaa gcgcgaggaa catgtagcca atctggctca tggtagagta agcgagaaca
     5221 cgtttgatgt cggtctgtac cagcgcggca aaaccggcca gcagcagcgt aaccgccccg
     5281 acaatgccca ccagatgcag aatttccggc gtcatcagga acaggccgtg ggtacgggcg
     5341 atcaggtaga cacccgcggt taccatggtt gcggcgtgga tcagcgcgga gacaggcgtc
     5401 gggcccgcca tcgcgtcggc aagccatgtc tgcaacggca actgcgcaga tttaccgacc
     5461 gcaccgccca gcagcatcag cgtcgcccac atcagcatgt tattgccgtc agcaaagtgc
     5521 gctggtgcca gttccaccat ttcgcggaag ttcagggtgc ccagttcgtt gtaaagaatg
     5581 aacagcgcga aagcgaggaa cacgtcaccc acacgggtca cgacgaacgc tttcattgcc
     5641 gctgcgccat tcttcggatc ggtgtaatag aacccgatca gcagatagga gcacaggccc
     5701 acgccttccc agccgaggta catcagcagc aggttatcgg cgagaaccag aactaccatg
     5761 ctggcgatga acaggttggt gtaggcgaag aagcgagagt agccctcttc accgcgcatg
     5821 taccaggagg cgtacatgtg aataaggaaa cccacaccag tgaccaccga gagcatggtc
     5881 agcgacaggc cgtccagcac caggttaaaa ccgatgttaa agtcgcctac cgacatccac
     5941 gtccacagcg gctggctgta tgcctgctcg ccgttagcga agaaatcaac gccgataaag
     6001 gcggttacca gcgccgccag gcccacagag ccaacgccga cgatcgccga aacgttttca
     6061 gaccagcgcc cacgggagaa cgccagcagg acgaagccaa tcaatggcaa aataatggtt
     6121 aaggcaagca tgttcatccg cgcatctcac ttactgaatc gatgttcagg ttctggcgac
     6181 gacggtgaag ttgcagcagc agcgcaaggc cgatactcgc ttctgccgcc gcgaggctga
     6241 tggcgagaat gtacatcacc tgaccgtcgg tctgacccca gtagcttccg gcgaccacaa
     6301 aggccagcgc ggaggcgtta atcatgattt ccagaccaat cagcataaac agcagattgc
     6361 gacggataac cagaccggtt aagccaagaa cgaataagat tgccgagagg atcagtccat
     6421 gttgtaaggg gatcatgcgt gctcctccgt ttttcttttc gcgctgtcgt ctttacgatt
     6481 gctcagcact tcacccgcac gctcttcacg accgacgtgg aaggccacaa ccagacccgc
     6541 gagcagcagc atagaagcca gttccaccgc cagtacgtaa ggcccgaaca gcgtaatacc
     6601 cactgcttta gcactgattg gcgtaccgtc gataccctga tcattaacac caaggatggc
     6661 gtaaacaatc accaccagca tgatggccga caaaattgcc ggaccaatcc acacctgcgg
     6721 tttcagccac tggcgttcct gttcgatttc tgaaccgccc aggttgagca tcatcaccac
     6781 gaacacgaac agcaccataa tggcacccgc gtagacgata atttccagcg caccggcgaa
     6841 gtaagcgccc agtgagaaga acaccccgga aatcgccagc agcgaaataa tcaggtacag
     6901 cagtgcgtgt accggattgg tatgggtgat cactcgcaag gtcgcaagta tggctatcag
     6961 gccacagata taaaaagcga actccattgc ccctctcctt acggtaacag gctcttgacg
     7021 tcgataggct tggcttcgtt ctctgcttcg cccttatctt tgccgtcgat tgccatacct
     7081 gccatccggt agaagttata ttccgggtat ttgcccggac cggagatcag cagatcctct
     7141 ttttcgtaaa ccagatcctg gcgcttgtat tcccccattt cgaaatccgg ggttaactga
     7201 atcgccgtgg tcggacaggc ttcttcgcac agaccacaga aaatgcagcg tgagaagttg
     7261 atgcggaaaa attccgggta ccagcgaccg tctttggttt ctgctttttg cagcgagata
     7321 cagccgaccg ggcaggctac cgcgcagagg ttacaggcta cgcaacgctc ttcgccgtcc
     7381 gggtcgcggg tcagaacgat acgaccacga taacggggcg gcagatagac cggctcttcc
     7441 gggtacattc gcgtttcgcg tttggcgaac gcgtgcaggc cgatcatcca gatactacga
     7501 acctgggtgc cgaaacctac taacaattct tttaaggtca tggtcttatt gccccttatt
     7561 gcgcctgcca gagaatgaca gccgccgtta ccagcaagtt gatcagcgtc agcggcaggc
     7621 agattttcca gccgaaggac attacctggt cataacgcgg acgcggtaac gacgcacgaa
     7681 tcaaaatgaa catcatcata aagaacgcgg ttttcaacgc gaaccagatg aatggcggta
     7741 acaacgggcc ttgccagcca ccgaagaaca gcgtcaccat caatgcagag atggtcacaa
     7801 tcccgatgta ctcacccacg aagaacagac cgaacttcat accggaatat tcaatgtggt
     7861 aaccatccgc cagttcctgc tcggcttccg gctggtcaaa cgggtgacgg tgacataccg
     7921 ccacgcccgc gatggcaaag gtaataaaac caaagaattg cgggataacg ttccacacat
     7981 gcgcctggct gttgacgatg tcggtcatgt tgaatgaacc ggcctgcgcc accacgccca
     8041 tcagggaaag cccgaggaac acttcgtagc tcagggtctg cgcagaagca cgcatcgcac
     8101 ccagcaacga gtatttgttg ttacttgacc agcccgcaaa cagcaccgcg taaaccgcca
     8161 gacctgccat catcaggaag aacaaaatcc cgatgttcag gtcggcaacc acccaacccg
     8221 gactgactgg cacaatcgca aaggccagca gcagcgaggt aaaggcaatc atcggtgcca
     8281 gggtaaagat gacgcgatcc gagaatttcg ggatccagtc ttctttaaag aacattttga
     8341 tcatgtccgc aaccagctgg agcgaaccgc cccagccaac acggttaggt ccataacggt
     8401 tctggaacag acccagcagg cgacgttcgc caaagctcat gaatgcccca caggtgacaa
     8461 ccaccagcag gatcaccacc gctttgagga tggtcagcag gatctcaatc agttccggtg
     8521 atatccaact cattgttgtg cctccttgag atcctcaaga tgcgcgccag ccagcaccgg
     8581 agcaatgccg gacataccca tcggcaagcc cacctgccct gccgtcagtc cttcggcgat
     8641 ttcaaccggc agcgtgaccg tgttgccatc gtaactaaag gagacgcgtg tacctgcgtt
     8701 cacacccaac ttcgcggcat ccgctgggtt gagtttgatg tacggctgcg gcatacggct
     8761 ctggaagacc ggagcacgct gtgacaattc atcgctgcca aacaggtggt aatacggcgc
     8821 aatacgccat ttcccgtcct gcggctggaa gcgtgccggt acgctggtga agtaatccag
     8881 accattctcg ctggtttcaa acagacgcac gcccggatcg ccaaagcgca gtttgccgcc
     8941 cacttcgtcc tggaatttgt tccacgcctg cggggagttc cagcccggtg cccaggcaaa
     9001 cggcacttgc gaacggtgcg cggtcggctg gttgttacct tccatcgaga aggtgaacat
     9061 ggtgtcaata tcctgcggct gacgcggctc atgaacgctg atattggcgc gcatggcggt
     9121 acgaccgctg taacggtgcg gttcacgggc cagtttctgc ccacgaatac ggaatgtcgc
     9181 atccggcgca gcatctttga tacctgccag ttccgggatt ttcgccacaa cagcgtcaat
     9241 cacatggtcg agctgcgtcc agtccacttc acggctcagc agggtgctgt gcagcgagtg
     9301 cagccagcgc cagctttcca gcatgacagt tttgctgtcg taataagccg gatcgtaaac
     9361 ctggaagaaa cgttgggcgc gaccttcatt attaattacc gtaccgtcgc tttcagcaaa
     9421 gctggcggcg gaaagtacca gatgggcgtt ttccatgatc gctgtacgct gatggtcaac
     9481 caccatcacc agcggtgctt tagccagcgc agcattcacg cgggtagcag aagcgtgacg
     9541 atgcagatcg ttttccaaca ccaccaccgc gtcggcgcgt ccggtttcca gttcggttaa
     9601 cgcttcttca agcgaaccgc cacccataat gcccagcccc atgctgttga cggaacgggc
     9661 aatcatggtg ataccgacgt cagcgccgcg acctttcagg gctttcgcga cgttagccgc
     9721 cgcctgaatc acctctaagc taccggcgtt cgtcccggag ataatcaacg gtttcttcgc
     9781 acctgccagt gcctgcacga tgacgtcgat tttgctttgc agctcaggtt cgataccgtc
     9841 aaccgctggt gcagagttat ccagcgcatg ggcgatggca aaacctaaac gcgcctgatc
     9901 ttcaaccggt gcgcggtaag tccacgccgc gatatcatcc agacgggtgt catcaacgtt
     9961 ggtaacaaac agcggatgct tcgcacgttg accgatgtta aggattgccg caatctgcca
    10021 gtcagccact ttctgtgctg ccgccatttc gcgcgcttta cctttcaccg cctgacgcac
    10081 tgccagcgcg acgcgcgcgc cggtctgggt aacgtcttca cccagcacca gtaccgcatc
    10141 gtaagattcg atttcgcgca gagccggagt ataaatgccg ccttcacgca gcactttcag
    10201 cgccagttgc agacgttcct gctcaccgtg agcgataccg gtgtagaagt tttcttcgcc
    10261 caccagttca cgcagtgcaa agttgctttc cacgctggca cgcggagaac caataccgat
    10321 cactttcttc gactgacgca gaatatctgc cgcgccctgc attgcctgtt cggcgttgag
    10381 ggtaatgaaa tcatcgccac gacgctgtac tggctgacgc ggacgatcct tcaggttgac
    10441 gtaaccgtaa ccgaaacgac cacggtcgca gaggaagtag tggtttaccg taccgttgta
    10501 acggttttcg atacgacgca gttcgccgta acgttcaccg gggctgatgt tacagccgat
    10561 ggaacattgc tggcagatgc tcggcgcaaa ctgcatatcc catttacggt tgtaacgctc
    10621 ggagtgcgtt ttgtcggtaa atacgccggt cgggcaaatt tcgaccaggt taccggaaaa
    10681 ttcgctttcc agcgtgccgt cttccgggcg accgaagtag acgttgtcgt gcgcgccata
    10741 aacgcccaga tctgtaccgt cagcgtaatc tttgtagtaa cgcacacagc ggtagcaggc
    10801 gatgcagcgg ttcatttcgt gagatatgaa tggccccaaa tcctgattac ggtgggtacg
    10861 tttggtgaaa cggtagcgac ggaagctgtg tccggtcatc acggtcatat cctgaagatg
    10921 gcagttaccg ccttcttcac ataccggaca gtcgtgcggg tggttggtca ttaaccactc
    10981 gaccacactt tcacggaact gtttcgcttc ttcgtcgtca atggaaataa aggtgccatc
    11041 ggaagccggt gtcatacagg acatcaccag gcgaccacgc gtgtcttccg cgttttggta
    11101 ttgcttcacc gcacactggc ggcaagcacc gacgcttccc agcgccggat gccagcaaaa
    11161 gtaaggaata tcaaggccca gagacagaca agcttccagc aggttgtccg ctccgttgac
    11221 ctcgtattct ttgccgtcta catgaattgt agccattagc atgcttccag ttttctcggt
    11281 cagggaccga gcgttaatcg aaattcggtt accagcgctc tttcagcagg ttcggctgaa
    11341 tcccattaat caaatgggta ttgctgaacg gctgtttgat tcccgcctca aattcttcgc
    11401 ggaaatattt gatggcgctc tgtaacggct ccactgcacc aggtgcgtgg gcacagaaag
    11461 ttttacccgg gcctaagaat cgacacagtt gctcaagtgt ttcaatatcg cccggctggc
    11521 cttcgccacg ctccagcgca cgcagaattt tcacgctcca cggcagaccg tcgcggcacg
    11581 gcgtacacca gccgcaggac tcacgggcga aaaactcttc caggttacgc accagcgaca
    11641 ccatgttgat ctcatggtca accgccatcg ccagcgccgt acccagacgg ctgcccgctt
    11701 taccgatact ttcgaattcc atcggcagat caaggtgcgc ttcggtcagg aagtcagtcc
    11761 ccgcgccgcc tggctgccag gctttaaatt tcagaccatc acgcatacca ccggcgtaat
    11821 cttcgaggat ctcgcgtgcg gtggtgccga acggcagttc ccacagtccc ggatttttca
    11881 cccgaccgga gaagcccatc agcttggtgc cagcatcttt acttttcgag atgttctgat
    11941 accactccac gccgttagcg aggatcgccg gaacgttaca cagggtttcg acgttgttga
    12001 cacaggtcgg tttgccccat acgccggagg ttgccgggaa ggggggtttc gagcgtgggt
    12061 tagcacgacg cccttctagg gagttgatta acgctgtttc ttccccgcag atgtagcgcc
    12121 ctgccccggt atggacgaac agttcgaagt cgaaacctgt tcccataatg tttttgccaa
    12181 gcaagcccgc ttcagtggct tcggcaatgg cgcggcgcag atttactgcc gcttcgatat
    12241 attcgccacg caggaagatg tagccacggt aagctttcag cgcaaacgcg gagatgagca
    12301 taccttccac cagcaggtgc ggcagttgct ccatcaacag gcggtcttta taggtgcccg
    12361 gctccatttc atcggcgtta cacagcaggt aacggatgtt catggattcg tctttcggca
    12421 tcaggctcca cttcaggcca gtagaaaagc ccgctccgcc gcgccctttc agaccagcgt
    12481 cttttacctg attaacgatt tcgtccggag acagcccggt cagcgcctta cgcgcccctt
    12541 cgtaaccgtt tttgctgcgg tattcgtcca gccacactgg ctgtttgtca tcgcgcagac
    12601 gccaggtcag cggatgcgtt tcgggagtac ggataatgtt tttcatttat accgctccag
    12661 cagttcaggg atcgcttccg gggtcagatg cgcgtgagtg tcctcatcga tcatcatgtt
    12721 tggcccttta tcacagttcc ccaggcagca agttggcagc agcgtaaagc ggccatcaaa
    12781 tgtcgtttgc cctggtttga tgttcagctt tttctcgagc gccgcctgaa taccctgata
    12841 cccgttgata tgacagacca cgctgtcgca ataacggatc acatggcgac caaccggctg
    12901 gcggaagatc tgactgtaga acgttgccac accttcgacg tcgcttgccg gaatacccag
    12961 cacatcggcg atcgcgtgga tcgcaccatc cggcacccag ccacgctgct tctgaacgat
    13021 tttcagcgct tcaatggacg ccgcacgcgg gtcttcgtag tggtgcatct cgtgctcaat
    13081 cgcttcacgc tctgccgcac tcagctcaaa agcctcggtt tgtggttgtt gattctcgtg
    13141 cataattagc ggtccacatc tgacataaca aaatcgatac tgcccagata aacaatcagg
    13201 tcagacacca ggctgccgcg gatcgccgcc ggaatttgct gcaaatgcgc ataactcggc
    13261 gtgcggatac gagtgcgata actcatggtg ctaccgtcgc tggtcaggta gtaactgttg
    13321 atccccttgg tcgcctcaat catctggaaa gattcattgg caggcatcac cggcccccac
    13381 gacacttgca ggaagtgggt gatcagggtt tcgatatgtt gcagcgtgcg ctctttcggc
    13441 ggcggcgtgg tcagcgggtg atccgctttg aacgggcctt ccggcatgtt gttgaggcac
    13501 tgctcaagaa tgcgcagact ctggcgcagc tcttccactt tcagcattac gcgggtgtag
    13561 cagtcagaaa caccaccacc aaccgggatt tcaaagtcga agttttcata gccagaataa
    13621 ggacgcgcct tacgcacgtc gaagtcgatc ccggtagcac gcaggcccgc gccagtggtg
    13681 ccccactcca gcgcctcttt cgcgccatag gcggcaacgc cctgggaacg acctttcaga
    13741 atggtatttt gcagcgccgc tttctcgtaa gacgccagac gtttcggcat ccagtcgagg
    13801 aactcacgca gcaggcgatc ccagccgcgc ggcaggtcgt gcgctacgcc gccaatacgg
    13861 aaccacgccg ggtgcatacg gaaacccgtg atcgcttcca ccagatcgta aattttctga
    13921 cgatcggtaa aggcgaagaa cacgggcgtc attgcgccga cgtcctgaat aaaggtcgag
    13981 atgtacagca ggtggctgtt gatacggaac agttcggaga gcataacgcg aatgacgtta
    14041 acgcgatccg gcacggtgat cccggccagt ttctctaccg ccagcacgta aggcatttcg
    14101 ttaacgcagc cgccgaggta ttcaatacgg tcggtatacg gaatgtagct gtgccaggac
    14161 tggcgctcgc ccattttctc agcaccacgg tggtggtagc cgatgtctgg tacgcagtcg
    14221 acaatctctt cgccgtcgag ttgtaaaacg atacggaaag ccccgtgcgc cgacgggtgg
    14281 ttcggaccga ggttgaggaa catgaagtcc tcgttttcgg tgccgcgctt catcccccac
    14341 tcttccggtt tgaaggtcag ggcttccatc tccagatcct gtttggcttt ggtcagctca
    14401 aacggcgaga attcggtagc gcgcgccggg taatctttac gcagcgggtg acctttccag
    14461 gtttgcggca tcatgatgcg gcgcaggttc gggtgaccgt cgaaagtaat gccaaacaga
    14521 tcccaggttt cacgctcata ccagttagcg ttcgggaaca gtttggtgaa ggtcggtacg
    14581 tgcaggtcgt tttctgccag cgccaccttc agcatgatgt cgcggttacg atcgatagaa
    14641 atcagatggt agaaaacgga aaaatccgcg gcaggtaacc cttcgcggtg tgtgcgcaga
    14701 cgttcgtcca tgccgtgtaa gtcaaacagc atgacgtaag gtttcggcag tttctttaag
    14761 aaatcgccaa cttccagtaa ttgttcacgc ttgatccaca caacgggaac cccggtgcga
    14821 gtcgcctgaa cagtaaaggc atccggccca aaacggttgc gcagttcgcc aattaccgga
    14881 tcatcaagat gatcgcgggt ctgccaggcg ggttcttgcg cggttaagtc ggtcatattg
    14941 ttcaccattg caaatggtcc gtggtgactg tcggcgcagt aagcttcgcg aaatagattt
    15001 aggaataagc gaagaaaaat ccctttgccg acaggcgcaa attaaatctc gtcaggtgta
    15061 cgcaggttgg ttacggcaat gcgttcaccg cgcttgcgtt cgcgctctga ttgcatattg
    15121 gcgcgataaa cgccctgatc gccaaccacc caggagagcg gacgacgttc tttgccgata
    15181 gattcctgca acagcatcag tgcctgcatg tacgcttcag gacgcggcgg gcagcccggg
    15241 atatacacat caaccgggat gaatttatcg acgccctgca caacggaata aatatcgtac
    15301 ataccaccag agttggcaca ggcacccatt gagataaccc attttggttc cagcatctgg
    15361 tcatacagac gctgaataac cggtgccatt ttggtaaagc aggttcctgc aaccaccatc
    15421 aggtcagcct gacgcggcga agcacgcaat acttctgcgc caaaacgcgc cacgtcatgc
    15481 accgcggtaa acgaagtcac catctcaacg taacagcagg aaagaccgaa gttatacggc
    15541 caaattgagt ttttacgacc ccagttaacc atgtcattga gcttgcccat aaacacgttt
    15601 ttgttaactt cttgctccag agggtcggtt acgatctcct gcttttgcag ggggtaacgg
    15661 tcgttctcac cgttgggatc tatgcgggtg agcgtataat ccatcttaat gcctcgcggt
    15721 tagcgttgac gattagcgat actgttcgtt tccgggttca tacgctcgcg gcgtgaacgc
    15781 gcgggcgtcc agtccagcgc gccaatacgc accagataaa ccagacctgc cagtaacaca
    15841 aaaataaaaa ttgcagcttc cacaaagcct acccagccgc tttcgcggat agaggttgac
    15901 catgcgaaca gatacagcgc ttcaacgtcg aagataacga agaacatggc caccagataa
    15961 aacttggcgg acaggcgtaa gcgggcggag ccgaccgagt cgataccgga ttcaaacggc
    16021 acgtttttcg acctcgcgcg tgcgcgaccg cctaaaaacc aaccacctac cagcattagg
    16081 caacacaggc caatggcaac gataagaaag atagcgaatg cccagtgatg agcgatgact
    16141 tcagtggatg ttgacatact cattgcttac tcatcaaaag tagcgccaga ttcactgctc
    16201 ttcacggcag atggacgcca catcgattca tggggaggaa taaaaaaaac cttacaatca
    16261 ctgtagaaat tcttttatac agctaattga tgtggtcttt tactcctttc tataaccttt
    16321 tgtcaacttt aacaaaagtt tcttcacatt agtttacata atatcaacac cattagcatt
    16381 taatgccctt tcaccccaga tccttgacga ctccaggata attagatgtt gttgaatcgt
    16441 gtccgttgtg aagcaatgga aaaaatacgg gtctattttg acaggaattt gtgtcgattc
    16501 ctccccccaa aagagagtat tttcttgatc tgtgacacgc ttttgtcatt ccataacaaa
    16561 aacgcagcaa caaatttacg tattttttaa catcattgta gcaggtgatt tttttcaggc
    16621 gattatttgt gcgttcggga cgtgaatctc tggtgattaa aaaatgaaca gttttgtacg
    16681 ttctgcacta tgcgaggaag gatttttact aaaaaaaagc cgctggggtt taaaacaccc
    16741 ccagcggctc gttttttaca ctattgtctc aggaattatc tatcgtccgt cgattactcg
    16801 atatcccttt caatcaacaa tgaatcatcc ccttccggag cagacattgg gctgtattgc
    16861 cacggattat ggtagctctc catcgcctga tagatcacct gggccagctc attattactg
    16921 gacgggtcat agcacagcaa atattcggta tcaggtagcg gcggtaagcc atctactcca
    16981 ctcagcacac gcaggtccgg gctcatcatc tcaaccggcc ttgccgtcac gccaagaccg
    17041 gctttcactg ccgcacgaac ggccggaagc gtcgaggcga cataagccag tcgccatgga
    17101 atatctgctt tattaagcgt tgccagcacc atatcgcgaa acggacttgg atcatccagc
    17161 aatacaaggg ggatcggctc gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt
    17221 gttggcgagg tacgcaggtt caatgcctta aacgctgaag gacgatgggt ggttaccatc
    17281 aaatccactt cctgggattc cagcatttct gccatatagg cattacgttt aacgcggaca
    17341 tccagcgcta atttcggata aaccgaactc acgcgattta acaggaaagg taagatcgta
    17401 tcggcagatt catctgaagc accgatagtt aacacgccct gaagattact gaacattaat
    17461 gatgagcagg cctcatcatt aaaacgcagg attttcctgg cgtaaccaag aagttgaatg
    17521 ccatgttcag ttaacagttt gttgcgaccg tgacgagcga acagttcttt cccaacgagt
    17581 tgttccagac gctgcatttg ctgacttacg gcggactgag tacgacacac agcggcagct
    17641 gcggcagcaa aagtgttcag atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg
    17701 aggttaatta tcggacgatt tgcacttatc atatattatc acttactggc ggctcatact
    17761 gagctggtta atgctgtgca cacacaaaca agcaattcca tttgtaatgt gcctccctgg
    17821 cagtttcatc cggaacccgg acgaaagtaa aaatgcatat gagttgcact aaaaaagcga
    17881 ctcacattgt tccgttataa tgcctgaagt agatcacaga atatatcttc agggatcgca
    17941 tatctattaa gttactcact cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa
    18001 atgtgagtcc ttgttccact ctcgtgcagc atcgctggtc atacgcgaac acgtacaaac
    18061 agcaatggtg aggcggcatc aagagcaggg atccgtcatt tatgcgagca ttttacccca
    18121 aaaacctttt atttataagg gccattgcga atcatctgat gcaaagttat gttatgttag
    18181 gcaaagtaat cttctattat taataagcac atcaaaacct ttttgaatat taaataataa
    18241 ttaattagca tcatcctcat tcattaattc cgcttaacaa tagtttcaca ccttgcccca
    18301 ttacgacacc tcggaaatca tcacgctgtt aaactctccg taatgcggaa atatcatcat
    18361 cgcgtttcat tagtgaattc ttaaatgagg cattttcaca caattatctt acagataaaa
    18421 aaaccagact tacaattaag aatcagaaca attcactata taacattgca tgtaaagcat
    18481 atacacctca ttattttgtc atcattaagt tattaacagc acaatcgagc cttcccctct
    18541 ggcaaaatct tattctgcag accttcaaaa caccgtcctg ggggagtaca ttgttctaag
    18601 ctgacttcca cggcagggag tggcgataac agcaaaaaag gtcaagattc atgtccccca
    18661 ttgaaaaatc cagcaaatta gagaatgtct gttatgacat ccgtggtccg gtgctgaaag
    18721 aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa actgaacatc ggcaacccag
    18781 ccccgttcgg ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc aacctgccta
    18841 ccgctcaagg gtattgcgat tccaaaggtc tttactccgc gcgtaaagcc atcatgcagc
    18901 actaccaggc tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc ggcaatggtg
    18961 tatcggagct tatcgttcag gcaatgcagg cattgctgaa cagcggggac gaaatgttgg
    19021 ttcctgcacc agattaccca ctatggaccg cggcggtttc gctttccagc ggtaaagcgg
    19081 tgcattatct ttgcgatgaa tcctctgact ggttcccgga cctcgatgat attcgcgcta
    19141 aaattacgcc tcgtacgcgt gggatcgtta ttatcaaccc aaataaccca accggcgcgg
    19201 tatattccaa agagctttta atggagattg tggagattgc acgtcagcat aatctcatta
    19261 tcttcgccga tgaaatttat gacaaaattc tttacgacga cgctgagcat cactcaattg
    19321 cgccgctggc acctgacctg ctgaccatta cctttaacgg actgtcgaaa acgtaccgcg
    19381 ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac gccaaaggct
    19441 acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt cctgcgcaac
    19501 acgccattca gaccgcgctg ggtggttatc agagcatcag tgaatttatt acccctggcg
    19561 gtcgtcttta tgagcagcgt aaccgcgcgt gggaactgat caacgatatt ccgggcgttt
    19621 cctgcgtgaa acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc aaacgcttta
    19681 acattcacga cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa gttctgttgg
    19741 tgcaagggac ggcattcaac tggccgtggc cggatcactt ccgcattgtc acgctaccgc
    19801 gtgtcgatga tatcgagctg tctttgagca agttcgcgcg tttcctttct ggttatcatc
    19861 agctgtaatc ttaatttcac tgccggagat tgcatccggc agcgttatcc cgccacaatg
    19921 acctgatgat gtcatcatac gtaaggtcac tatgaaacag agccattttt ttgcccatct
    19981 ctcccgcctg aaactcatta accgctggcc gctaatgcgc aacgtgctga cggaaaatgt
    20041 gtccgaacac agtttgcagg tagcgatggt cgcccatgcg ctggcagcta tcaaaaatcg
    20101 aaaatttggc ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt accacgatgc
    20161 ctcagaagtg ctcaccggcg atctccctac tccggtgaaa tacttcaatt cgcaaatcgc
    20221 tcaggagtac aaggctattg aaaaaatcgc tcagcaaaaa ctggtcgata tggttccgga
    20281 agagctgcgg gatatctttg cgccgttaat tgacgagcat gcatatagcg atgaagaaaa
    20341 atcgctggtg aaacaggcag atgcactgtg tgcatatctg aaatgtctgg aagaactcgc
    20401 ggccggaaat aatgaattct tgctggcaaa aacgcgactg gaagcgacgc ttgaagcgcg
    20461 tcgcagccag gagatggact acttcatgga agtatttgtt cccagcttcc atctttcgct
    20521 cgatgagatt agccaggatt caccgctgta agcagccgga gtctgcgtcg catcaggcaa
    20581 taagcgccgg atgcgacatc aggctcttgt caaaacggaa acagcatcgg gatcatcacc
    20641 acacaaaccg ccatcacgat aatggtgaac ggtaccccca acttcacaaa gtcactgaag
    20701 ctgtaatttc ccggacctaa aaccagtgtg ttaacaggtg aagaaaccgg tgtcataaag
    20761 gcggcggatg ctgccatcgc cacgaccatc gcgaatggat aaggcgacac ccccatcgtt
    20821 ttggcagcag ccagcgcaat cggagccatc aacaccgccg tcgcggtatt agagataaat
    20881 agcccaataa ccgccgacaa gacaaacaaa caccccagca tcatatgtgg cccgtaacca
    20941 ccgccaatgt ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt ctgtaatgcc
    21001 acagcaaacg gcatcatccc aacgatcaaa ataatgctcg gccagtgaat ggatttatag
    21061 gcgctttcag catctataca gcggaatttc cccatcagca ggcaggcgat gatagcggca
    21121 acaggattag gaatttcatc tgtcagcatt aacgccacca ttagcaccag acagaaaatg
    21181 gcatggggtg cctggctgtg cgcgggcgat gcttcactca cctcttccgg taagttcagc
    21241 gctacgaagt cgcggccctg tttggccagc ataccgatca gtttccagtt acccacaacc
    21301 aggatgatat cgcccagcag cagaggctca tccgccagcg aaccttccag cgccacgcca
    21361 ttgcgcttta gccccaccac attcagtccg tagcgggtac gaaaaccaat ttcgcgcacc
    21421 gatttaccaa tcagttctga ctcaggaatt aatgaaatct ctgccatgcc cacatcaagg
    21481 gcctggtcag aaaaatactc gccgcgcagt accatcggct ccagcaattg ctcactacaa
    21541 aattgccgga gatcgacatc agccgcagac atatcaataa gcaaaacgtc acgcgcgcga
    21601 aattcagaaa ccccattaac gttcacgata acgcgacgaa aacgccgcca gcgttcaaca
    21661 ccgatgacgt tagcgccata acgctcacgt aatttgagat catccagccg ttgaccaatc
    21721 attggcgatc cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg atattcacgg
    21781 ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg ggtatcccct
    21841 ttcagcatga aacgcatcac taacatatac aaaataccca gcaccagcac gaccaggcca
    21901 acaggtgtta cgctaaagaa actgaagccg tgatagcctt cacgcagcaa ttcactgttg
    21961 actaccaggt tcggcggcgt cgccaccagc gtcatcatgc cgctaatcag cccggcaaaa
    22021 cttaacggca tcatcagacg tgacggcgaa gtttgcatac gcatggcaac gcttaacacc
    22081 acgggaataa agatagcgac aacgccggtt gaactcataa acgcgccaag ccccgcgacg
    22141 gtcagcatca acaaaaccaa cattttgatt tcactgttgc ccgccacttt gaccagccat
    22201 gttcccatta cggtggcaac accggtacgg accaaaccat cgccaataat aaacaaggcg
    22261 gcaatcagga caacgttagg atcagaaaag ccggaaaata cttcggggac cgtcagcgtt
    22321 ccgcttaatg caaacgcgac aataacaaac aaagcgaccg catccatacg cactctgccc
    22381 gtcgcaaaca agacgatggc aacggccagt aatgaaagaa cccaaatcaa ttcaccgttc
    22441 acaacttatc cttgttaatt gagggggatg acttgattct gccataaaaa agccccgacg
    22501 agacggggct aaatcatgat caggtgtttc actgaataat aacatcgcca tttagctgtt
    22561 tggtcacagt aatttgctcc agactgtgga ggaccaaatc gacctcattc aggcgcgggg
    22621 tatctgccgg agcgttaacc gcaatgacat ggcaacccgc cgccaggcca gaaagcacgc
    22681 cagcgggagc atcttccgcc accacacact cctgcggcgc aagccccagc agctgcgcac
    22741 ctaacagata cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg gttacaaaca
    22801 cctttggtgc gggaagccca gctattttat ggcgcgctcg cgctaccggc atggagccag
    22861 aagtcacaat ggcccacgga atacctgctt tattcaaatg attgagtaag gcgattgccc
    22921 ccggaagcgc ggtaatacct tcggtttccg tggcctcgat gtgctccaga cacgtaaact
    22981 cggcggcaat atcagcctcg gatttgcccg ccataaaatg gcgcaaagag gtgatcgcct
    23041 gtttaccgtg aatgaaagcc agcacctctt ccggcgctaa cccatgacgt ctggcccagt
    23101 tgctccacgc ccgttctacc gcaggcaggg aatccaccag cgttccatca agatcaaaca
    23161 gaaaaccttt gcaccgcacg cgggcctcct caggcgttga taatttgatt aatttcgttg
    23221 gcgctcaaat ggtactgacg caggcaggca tgccacacat taagcatgcg ctgatatttt
    23281 tcccacattg gcgtctgggc gttaaaacca tgagtcccag cgtcaaaatg ggtatagcgc
    23341 ccttccacat taaccataaa gcggacataa ccgaggtaac gtgcttcagt ggcggcgtca
    23401 aagccgagga aggtgacacg acgttcatcg atggattgct ggtcctgcaa attagaccag
    23461 gaaacatgca acgcatgata catctccata atgtcgatga tagtgcggca ggtttcttct
    23521 ttcagctcgc caaactcgcg atccaattca cgcatctgta atccgtaacc acgctcaata
    23581 attgtttgca ggcgacggta acgttcagca tttgccggat cgagcatagt catcatcttg
    23641 tactgattag acaaaataag acgttgcgcg ttggtcattt ccattgttga ctcctgtatc
    23701 actctactac ggtgaaaaaa aaagaaggct gagtatgcct tcttttatat gcgtaatcag
    23761 gggtcaatta caaatcatca aggaaagttt tatccagttg tttgaaggcg cgcttaagcg
    23821 tgtcagctaa tgcctggtaa tcaggcttgc cttcaacggg tgccaacacc tgtccagact
    23881 cctgcaattt accgcgaact tcataaaacc agttgaggat agcagggggt aatggcgtta
    23941 cagaacgctt gcccagccac cataatccct gcatgggtaa acttaaggcg aacagcgcag
    24001 tggcaactgc cggcccaagc tgaccgccca gggcaatctg ccagcagaga gtaaatacgg
    24061 cgatcggcgg cataaaacgg atcgcataac gcgtcatctt gataacgcga ttttcgacaa
    24121 agaccggggc aaggcgtttt tccagcggcc acgtctttga gtaatgctgt ccccggcgaa
    24181 acaagctaaa aaaattaaca gaacgattat ccggcgttga catgcttcac ctcaacttca
    24241 catataaaga ttcaaaaatt tgtgcaaatt cacaactcag cgggacaaca ttcaaaacat
    24301 tttgtcttcc atacccacta tcaggtatcc tttagcagcc tgaaggccta agtagtacat
    24361 attcattgag tcgtcaaatt catatacatt atgccattgg ctgaaaatta cgcaaaatgg
    24421 catagactca agatatttct tccatcatgc aaaaaaaatt tgcagtgcat gatgttaatc
    24481 ataaatgtcg gtgtcatcat gcgctacgct ctatggctcc ctgacgtttt tttagccacg
    24541 tatcaattat aggtacttcc atgtcgagta agttagtact ggttctgaac tgcggtagtt
    24601 cttcactgaa atttgccatc atcgatgcag taaatggtga agagtacctt tctggtttag
    24661 ccgaatgttt ccacctgccc gaagcacgta tcaaatggaa aatggacggc aataaacagg
    24721 aagcggcttt aggtgcaggc gccgctcaca gcgaagcgct caactttatc gttaatacta
    24781 ttctggcaca aaaaccagaa ctgtctgcgc agctgactgc tatcggtcac cgtatcgtac
    24841 acggcggcga aaagtatacc agctccgtag tgatcgatga gtctgttatt cagggtatca
    24901 aagatgcagc ttcttttgca ccgctacaca acccggctca cctgatcggt atcgaagaag
    24961 ctctgaaatc tttcccacag ctgaaagaca aaaacgttgc tgtatttgac accgcgttcc
    25021 accagactat gccggaagag tcttacctct acgccctgcc ttacaacctg tacaaagagc
    25081 acggcatccg tcgttacggc gcgcacggca ccagccactt ctatgtaacc caggaagcgg
    25141 caaaaatgct gaacaaaccg gtagaagaac tgaacatcat cacctgccac ctgggcaacg
    25201 gtggttccgt ttctgctatc cgcaacggta aatgcgttga cacctctatg ggcctgaccc
    25261 cgctggaagg tctggtcatg ggtacccgtt ctggtgatat cgatccggcg atcatcttcc
    25321 acctgcacga caccctgggc atgagcgttg acgcaatcaa caaactgctg accaaagagt
    25381 ctggcctgct gggtctgacc gaagtgacca gcgactgccg ctatgttgaa gacaactacg
    25441 cgacgaaaga agacgcgaag cgcgcaatgg acgtttactg ccaccgcctg gcgaaataca
    25501 tcggtgccta cactgcgctg atggatggtc gtctggacgc tgttgtattc actggtggta
    25561 tcggtgaaaa tgccgcgatg gttcgtgaac tgtctctggg caaactgggc gtgctgggct
    25621 ttgaagttga tcatgaacgc aacctggctg cacgtttcgg caaatctggt ttcatcaaca
    25681 aagaaggtac ccgtcctgcg gtggttatcc caaccaacga agaactggtt atcgcgcaag
    25741 acgcgagccg cctgactgcc tgatttcaca ccgccagctc agctggcggt gctgttttgt
    25801 aacccgccaa atcggcggta acgaaagagg ataaaccgtg tcccgtatta ttatgctgat
    25861 ccctaccgga accagcgtcg gtctgaccag cgtcagcctt ggcgtgatcc gtgcaatgga
    25921 acgcaaaggc gttcgtctga gcgttttcaa acctatcgct cagccgcgta ccggtggcga
    25981 tgcgcccgat cagactacga ctatcgtgcg tgcgaactct tccaccacga cggccgctga
    26041 accgctgaaa atgagctacg ttgaaggtct gctttccagc aatcagaaag atgtgctgat
    26101 ggaagagatc gtcgcaaact accacgctaa caccaaagac gctgaagtcg ttctggttga
    26161 aggtctggtc ccgacacgta agcaccagtt tgcccagtct ctgaactacg aaatcgctaa
    26221 aacgctgaat gcggaaatcg tcttcgttat gtctcagggc actgacaccc cggaacagct
    26281 gaaagagcgt atcgaactga cccgcaacag cttcggcggt gccaaaaaca ccaacatcac
    26341 cggcgttatc gttaacaaac tgaacgcacc ggttgatgaa cagggtcgta ctcgcccgga
    26401 tctgtccgag attttcgacg actcttccaa agctaaagta aacaatgttg atccggcgaa
    26461 gctgcaagaa tccagcccgc tgccggttct cggcgctgtg ccgtggagct ttgacctgat
    26521 cgcgactcgt gcgatcgata tggctcgcca cctgaatgcg accatcatca acgaaggcga
    26581 catcaatact cgccgcgtta aatccgtcac tttctgcgca cgcagcattc cgcacatgct
    26641 ggagcacttc cgtgccggtt ctctgctggt gacttccgca gaccgtcctg acgtgctggt
    26701 cgccgcttgc ctggcagcca tgaacggcgt agaaatcggt gccctgctgc tgactggcgg
    26761 ctacgaaatg gacgcgcgca tttctaaact gtgcgaacgt gctttcgcta ccggcctgcc
    26821 ggtatttatg gtgaacacca acacctggca gacctctctg agcctgcaga gcttcaacca
    26881 ggaagttccg gttgacgatc acgaacgtat cgagaaagtt caggaatacg ttgctaacta
    26941 catcaacgct gactggatcg aatctctgac tgccacttct gagcgcagcc gtcgtctgtc
    27001 tccgcctgcg ttccgttatc agctgactga acttgcgcgc aaagcgggca aacgtatcgt
    27061 actgccggaa ggtgacgaac cgcgtaccgt taaagcagcc gctatctgtg ctgaacgtgg
    27121 tatcgcaact tgcgtactgc tgggtaatcc ggcagagatc aaccgtgttg cagcgtctca
    27181 gggtgtagaa ctgggtgcag ggattgaaat cgttgatcca gaagtggttc gcgaaagcta
    27241 tgttggtcgt ctggtcgaac tgcgtaagaa caaaggcatg accgaaaccg ttgcccgcga
    27301 acagctggaa gacaacgtgg tgctcggtac gctgatgctg gaacaggatg aagttgatgg
    27361 tctggtttcc ggtgctgttc acactaccgc aaacaccatc cgtccgccgc tgcagctgat
    27421 caaaactgca ccgggcagct ccctggtatc ttccgtgttc ttcatgctgc tgccggaaca
    27481 ggtttacgtt tacggtgact gtgcgatcaa cccggatccg accgctgaac agctggcaga
    27541 aatcgcgatt cagtccgctg attccgctgc ggccttcggt atcgaaccgc gcgttgctat
    27601 gctctcctac tccaccggta cttctggtgc aggtagcgac gtagaaaagg ttcgcgaagc
    27661 aactcgtctg gcgcaggaaa aacgtcctga cctgatgatc gacggtccgc tgcagtacga
    27721 cgctgcggta atggctgacg ttgcgaaatc caaagcgccg aactctccgg ttgcaggtcg
    27781 cgctaccgtg tttatcttcc cggatctgaa caccggtaac accacctaca aagcggtaca
    27841 gcgttctgcc gacctgatct ccatcgggcc gatgctgcag ggtatgcgca agccggttaa
    27901 cgacctgtcc cgtggcgcac tggttgacga tatcgtctac accatcgcgc tgactgcgat
    27961 tcagtctgca cagcagcagt aatctcgtca tcatccgcag ctttgcgctg cggatatctg
    28021 aaccggaaat aatcactatt tccggttttt tattctctta atctgcatta atcctttctg
    28081 attatcttgc ttaactgcgc tgcatcaatg aattgcgcca tttcactttg catacttacc
    28141 actttgtttt gtgcaaggga atatttgcgc tatgtccgca atcactgaat ccaaaccaac
    28201 aagaagatgg gcaatgcccg atacgttggt gattatcttt tttgttgcta ttttaaccag
    28261 ccttgccacc tgggtagttc cggtggggat gtttgacagt caggaagtgc agtatcaggt
    28321 tgatggtcaa acaaaaacac gcaaagtcgt agatccacac tcatttcgca ttctgactaa
    28381 cgaagcaggc gaacctgagt atcaccgcgt acagctgttc acgacgggcg atgaacgccc
    28441 gggcctgatg aacttcccgt ttgaaggatt aacctcagga tcgaaatacg ggacagctgt
    28501 tggcatcatc atgtttatgc tggtgattgg cggcgcgttt ggcattgtga tgcgtacagg
    28561 aaccattgat aacggtatcc tggcgcttat tcgccatacc cgcgggaatg aaattctctt
    28621 tattcctgcg ctgtttattc tgttttcact tggcggcgcg gtatttggta tgggagaaga
    28681 ggccgtcgcc tttgccatta tcatcgcacc gctaatggtc cggctgggct atgacagtat
    28741 taccaccgtc ctggtgacct atattgccac gcaaatcggt tttgccagtt cgtggatgaa
    28801 cccgttttgt gtggtcgttg ctcaggggat tgccggcgtt ccggtgcttt ctggctccgg
    28861 gttgcgcatc gtggtgtggg ttatcgccac tatgattggc ctgatcttta ccatggtgta
    28921 cgcctcacga gtgaaaaaga atcctcttct gtcacgcgtg catgagtccg accgcttctt
    28981 tcgtgaaaag caggcggatg ttgaacaacg tccgtttacc tttggtgact ggctggtatt
    29041 gattgtcctg accgccgtaa tggtctgggt gatttggggc gtgatcgtta atgcctggtt
    29101 tattccagaa attgccagcc agttcttcac catgggtctg gtgattggca tcatcggcgt
    29161 tgtttcccgc cttaacggca tgacggttaa taccatggct tcatccttta ccgaaggggc
    29221 gcgaatgatg atcgcccctg ccctgctggt gggtttcgcc aaagggattt tgctgctggt
    29281 cggtaatggt gaagcgggtg atgccagcgt gttaaatacc atcctcaaca gcattgccaa
    29341 tgccattagc ggtctggaca acgcggtcgc ggcctggttt atgttgctct tccaggcggt
    29401 atttaatttc ttcgtgacgt ccggttctgg tcaggcggcg ttaaccatgc cgttactggc
    29461 accgcttggc gatctggtcg gtgttaaccg tcaggttacc gtgctggcat tccagtttgg
    29521 tgatggcttc agtcacatca tttacccaac ctcagcttcg ttaatggcaa ctctcggtgt
    29581 gtgcagggtg gacttccgta actggctgaa ggtgggcgcg accctacttg gactgctgtt
    29641 tattatgtcc agcgtcgtag tgatcggcgc tcagttgatg ggctaccact aaaaatgtta
    29701 agagccgcaa ttgcggctct tttttcattc tgccgtttca gtctctacgg cttcattttt
    29761 ggcattgcgc ttcatccaca acgccagcgc tttcagcgag tctggagtga actcatcgca
    29821 gcgtgcggtg atttcttccg gcgtcagcca gcaaacttca ctgacttcat cttcctgtag
    29881 ggcgaaggga ccgtgagaga cgcagctgaa caacgcgccc cagacacggc aatttttatc
    29941 ttcgaaatag aactgcccgt gctcggcaaa ggggacaccg gcaatgccca actcttcttc
    30001 cgcttcgcga cgcgcggatt ccagcagttg ctcatcggcc tgaaccacac cgcctgcggt
    30061 cgcatctaac atgccgggta aaaagtcttt tgtctcggta cgacgctgga ccagaatttt
    30121 gcccatgcca tcatgcacga cgatgtaagt tgcacgatga cgcagacact gtgcccgcat
    30181 ttgttcccgg ctggcttgtg caatgacttc gttctcttca ttgacaatat ccacccattc
    30241 agtacttgcc aaacgacgct gttccattat caggaaacct tctttttctg gcgctcttac
    30301 ggcgcatttt gagttgtggg taaattacgg attaatcgcg acctgcgcaa tgatactttg
    30361 atcattgagt gcgattacac taagtacgtc attatccagc atgccataac tcgccggatt
    30421 accgcctttc ggaatactca ccgagccggg gttgaagtgg aaaatctccc cccgctgttc
    30481 tgccactggc agatgggtat gaccgtacac cagcacatcg ttctggttta aagcaggtag
    30541 attttccggg ccaaaaagat gaccatgcgt caaaaacaga cgttgttttt ccagtaatac
    30601 ctgttgccac ggcgcggtta tcgggaaatg cagcagcatt tgatccactt cgctgtcgca
    30661 gttgccgcgc acagcgataa ccttatgtgc cacttcatta agccgttcag cgactttggc
    30721 tggcgcgtaa ccctccggta aagcattacg cgggccgtga ttcaacacgt cgccaaggat
    30781 caccagccac tgggcaccgc tttgggcaaa caactccaga acacgttccg tcgccggtaa
    30841 cgacccatga atgtccgatg caaacatcag tttcatcact cactcctcgt cgaaaaaacg
    30901 taagctatga tactggattc tgtcgttgct atcagccaga ttgcttcgcc gaaagtgcaa
    30961 taaaacgctg gacagacgct cgctgccact ggaatgtcgc ataatccacc aggcgttctg
    31021 gcacctcatc gccatgtagt accaggcggt taatcattag cgccagatca gtatcagcaa
    31081 tgcaccattc accaaataaa ttcggctgac ccagtgctaa caaatgttct gccatcgcga
    31141 cactggcttt tccctcggcc gttagtggcg cttttttcgc ccccgcaaag acaacatccg
    31201 tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga atctgtcgtg
    31261 cacgcgcacg attttctaaa tcaagcggat aaatacgttc ccaggtcggt ggcgcaaatc
    31321 gatcttccag atactccgca atggcagaag attcactcaa ctcaaaatca tcgatttgta
    31381 ataacggcac acggcgtgtc tgaccgtaac cttgccacgt cggctgcaaa tgttcaccgc
    31441 tgtcgaggtc gatggtcttg atatgaaacg acaggccttt ttcctgcaac gccacccaag
    31501 cggataacac ataaggggag aaaaagtggg catctgacca aagcgtgata gcgggtttac
    31561 tcattatgtc ctcggcaata aaggaggctg gctatcaaac tatagagcct ttttgtcact
    31621 gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta tacttgtggt gttcagatga
    31681 ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca atggtcacaa
    31741 aattacgctg tttctcgaag aagcagagct tgattatcgc ttgattaagg tagacctggg
    31801 gaaaggcggt cagtttcgtc cggaattttt gcgcatttcg cccaacaaca aaattccggc
    31861 aattgttgat cattctcctg ccgatggcgg cgaaccgcta agcctctttg aatctggtgc
    31921 cattttgttg tatctggctg agaaaacggg gctctttttg agtcatgaaa cgcgtgaacg
    31981 cgccgccaca ttacagtggt tattctggca ggtaggcgaa ctggggccga tgcttgggca
    32041 aaatcatcat tttaatcacg cagcccccca aaccattcct tacgctattg aacgttatca
    32101 ggttgaaact cagcgtcttt accatgtact aaacaagcgg ctggaaaact cgccctggct
    32161 gggaggcgag aactacagca ttgcggatat tgcctgctgg ccgtgggtta atgcctggac
    32221 tcgccagcga attgacctcg caatgtatcc ggcagtcaag aactggcatg agcggatccg
    32281 ttcgcgccct gccaccgggc aggcactgct aaaggcacaa ctcggtgatg agcgttcgga
    32341 tagttaacag aaacaggttc tcgtgtatta tttcatccta agtaaaacaa cggagaacct
    32401 gcaatggcac aacctgccgc tattattcgt ataaagaacc ttcgtttacg tacgtttatc
    32461 ggaattaagg aagaagaaat taacaaccgt caggatattg ttatcaatgt gacgatccac
    32521 taccccgccg ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa ttatcgcacc
    32581 gtaacgaaaa acattattca gcatgtagag aataaccgtt tctctttgtt ggaaaaatta
    32641 actcaggatg tgctcgatat cgcacgtgaa catcactggg tgacgtatgc tgaagtggag
    32701 atcgataaac tgcacgcgct gcgctacgcc gattcggtat ccatgacctt aagctggcag
    32761 cgttaaccgc catatcggga ggctgcatga acatagtgat caccggaggg acgggattaa
    32821 ttggtcgcca tttgattccg cgtttgctgg agctgggcca tcaaatcacg gtagtgacgc
    32881 gtaacccaca gaaagccagt tccgttctcg gccctcgggt gacgctatgg caagggctag
    32941 ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa cctggccgga gaaccgattg
    33001 ctgataaacg ctggactcac gagcaaaaag agcgtctctg ccaaagccgc tggaatatca
    33061 cgcaaaaact ggtcgatttg attaatgcca gcgacacgcc gccgtcggta ctcatttccg
    33121 gctcggcaac gggctattat ggcgacttag gtgaagtggt ggttaccgaa gaggaaccgc
    33181 cgcataacga atttactcat aaactctgcg cccgctggga agagattgcc tgccgggcgc
    33241 aaagtgacaa aacgcgagtg tgcctgctgc gaaccggcgt ggtactggca ccggacggcg
    33301 gtattctcgg taaaatgctg ccaccgtttc gtcttggcct gggcgggccg attgactctg
    33361 gtcggcagta tctggcctgg attcatattg atgatatggt caacggtatt ctctggctgc
    33421 tggataacga gctgcgcggg ccatttaata tggtttcgcc ctacccggta cgcaatgaac
    33481 aatttgccca tgcgctcggt cacgcgctgc atcgcccggc cattttgcgc gtccccgcga
    33541 ccgccattcg actgttaatg ggcgaatctt cagtactggt attaggtgga caacgcgcgc
    33601 tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg gtacgattta gaagaggcgc
    33661 tggcggatgt cgttcgctga tgtggtttac agcaaacatc cgccagttaa ctcccggtgt
    33721 tacaggatta gtggctttgc gcgataagat cgtctggtga aagtcgggtc accatcataa
    33781 ctaactctct gtctaaacct ctatccagca tctcctgagc aatacgcagg gcttcttcgt
    33841 gtttgccctg cattgcgcct tcttcacgta atctgtcagc aatggtcatc agtttctcct
    33901 tttcttgtgg tgcgcgttcc gctatctcac caataaatgc acgaaaacgc tgggcatccc
    33961 ctgtttgtaa tacgtaatta aacagggctt ttagctgtct gtcattagtg ttccctgtaa
    34021 ctagcagcga aacaatttgg tcgactaatc ccaacagatc gcgctgacga atatgtttct
    34081 gaattaactc caacagcgcc attttgcggt gttgcataat ctcgtcatcc ggcaccacgg
    34141 taatatccac caacggaaac gctgatgaat atattttgcg ggctatagca ggctcggcaa
    34201 attcatcaag ccagcagagt gaataaggat aaggacttct gcaaccatga taaaacagca
    34261 tcgggatcac caatggaagc tctttatagc ccgc
//
LOCUS       DAJTOR010000026        33947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTOR010000026 DAJTOR010000000
VERSION     DAJTOR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.026"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(178..390)
                     /gene="rpmE"
                     /locus_tag="OO249_001094"
     CDS             complement(178..390)
                     /gene="rpmE"
                     /locus_tag="OO249_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5635466.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            593..2791
                     /gene="priA"
                     /locus_tag="OO249_001095"
     CDS             593..2791
                     /gene="priA"
                     /locus_tag="OO249_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5635467.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2947..3972
                     /gene="cytR"
                     /locus_tag="OO249_001096"
     CDS             2947..3972
                     /gene="cytR"
                     /locus_tag="OO249_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5635468.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4064..5023
                     /gene="ftsN"
                     /locus_tag="OO249_001097"
     CDS             4064..5023
                     /gene="ftsN"
                     /locus_tag="OO249_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5635469.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5116..5646
                     /gene="hslV"
                     /locus_tag="OO249_001098"
     CDS             5116..5646
                     /gene="hslV"
                     /locus_tag="OO249_001098"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5635470.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5656..6987
                     /gene="hslU"
                     /locus_tag="OO249_001099"
     CDS             5656..6987
                     /gene="hslU"
                     /locus_tag="OO249_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5635471.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7054..7980
                     /gene="menA"
                     /locus_tag="OO249_001100"
     CDS             7054..7980
                     /gene="menA"
                     /locus_tag="OO249_001100"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5635472.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDRPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8073..8558
                     /gene="rraA"
                     /locus_tag="OO249_001101"
     CDS             8073..8558
                     /gene="rraA"
                     /locus_tag="OO249_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5635473.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8643..8888)
                     /gene="zapB"
                     /locus_tag="OO249_001102"
     CDS             complement(8643..8888)
                     /gene="zapB"
                     /locus_tag="OO249_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5635474.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9314..10159
                     /gene="glpF"
                     /locus_tag="OO249_001103"
     CDS             9314..10159
                     /gene="glpF"
                     /locus_tag="OO249_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR5635475.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVKEKETTTPSE
                     QKASL"
     gene            10182..11690
                     /gene="glpK"
                     /locus_tag="OO249_001104"
     CDS             10182..11690
                     /gene="glpK"
                     /locus_tag="OO249_001104"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5635476.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRSE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11825..12835
                     /gene="glpX"
                     /locus_tag="OO249_001105"
     CDS             11825..12835
                     /gene="glpX"
                     /locus_tag="OO249_001105"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5635477.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12932..13678
                     /gene="fpr"
                     /locus_tag="OO249_001106"
     CDS             12932..13678
                     /gene="fpr"
                     /locus_tag="OO249_001106"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5635478.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIG
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13683..14111)
                     /gene="uspD"
                     /locus_tag="OO249_001107"
     CDS             complement(13683..14111)
                     /gene="uspD"
                     /locus_tag="OO249_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5635479.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14138..14437)
                     /locus_tag="OO249_001108"
     CDS             complement(14138..14437)
                     /locus_tag="OO249_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5635480.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSVDEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14649..15089)
                     /locus_tag="OO249_001109"
     CDS             complement(14649..15089)
                     /locus_tag="OO249_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5635481.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     CFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15190..15789
                     /locus_tag="OO249_001110"
     CDS             15190..15789
                     /locus_tag="OO249_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5635482.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15897..16664
                     /gene="tpiA"
                     /locus_tag="OO249_001111"
     CDS             15897..16664
                     /gene="tpiA"
                     /locus_tag="OO249_001111"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5635483.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEHGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16719..17474)
                     /gene="cdh"
                     /locus_tag="OO249_001112"
     CDS             complement(16719..17474)
                     /gene="cdh"
                     /locus_tag="OO249_001112"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5635484.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17581..18570)
                     /gene="sbp"
                     /locus_tag="OO249_001113"
     CDS             complement(17581..18570)
                     /gene="sbp"
                     /locus_tag="OO249_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5635485.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18890..19852)
                     /gene="pfkA"
                     /locus_tag="OO249_001114"
     CDS             complement(18890..19852)
                     /gene="pfkA"
                     /locus_tag="OO249_001114"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5635486.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20033..20935)
                     /gene="fieF"
                     /locus_tag="OO249_001115"
     CDS             complement(20033..20935)
                     /gene="fieF"
                     /locus_tag="OO249_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5635487.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21083..21595)
                     /gene="cpxP"
                     /locus_tag="OO249_001116"
     CDS             complement(21083..21595)
                     /gene="cpxP"
                     /locus_tag="OO249_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5635488.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQRS
                     TQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQ
                     AEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLK
                     LLSSSNSRSQ"
     gene            21745..22443
                     /gene="cpxR"
                     /locus_tag="OO249_001117"
     CDS             21745..22443
                     /gene="cpxR"
                     /locus_tag="OO249_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5635489.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22440..23813
                     /gene="cpxA"
                     /locus_tag="OO249_001118"
     CDS             22440..23813
                     /gene="cpxA"
                     /locus_tag="OO249_001118"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5635490.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23919..24593)
                     /gene="yiiM"
                     /locus_tag="OO249_001119"
     CDS             complement(23919..24593)
                     /gene="yiiM"
                     /locus_tag="OO249_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5635491.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24742..25707)
                     /gene="kdgT"
                     /locus_tag="OO249_001120"
     CDS             complement(24742..25707)
                     /gene="kdgT"
                     /locus_tag="OO249_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5635492.1"
                     /translation="MRKSRGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMITGTVSI
                     LAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVEVGFFAG
                     LSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGTAGIASF
                     EPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTVIAQTGL
                     LGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPMLIAEMVPAFKP
                     MAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25986..26606)
                     /gene="sodA"
                     /locus_tag="OO249_001121"
     CDS             complement(25986..26606)
                     /gene="sodA"
                     /locus_tag="OO249_001121"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5635493.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26890..27924
                     /gene="rhaT"
                     /locus_tag="OO249_001122"
     CDS             26890..27924
                     /gene="rhaT"
                     /locus_tag="OO249_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR5635494.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27921..28787)
                     /gene="rhaR"
                     /locus_tag="OO249_001123"
     CDS             complement(27921..28787)
                     /gene="rhaR"
                     /locus_tag="OO249_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5635495.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(28843..29679)
                     /gene="rhaS"
                     /locus_tag="OO249_001124"
     CDS             complement(28843..29679)
                     /gene="rhaS"
                     /locus_tag="OO249_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5635496.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29967..31379
                     /gene="rhaB"
                     /locus_tag="OO249_001125"
     CDS             29967..31379
                     /gene="rhaB"
                     /locus_tag="OO249_001125"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5635497.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            31376..32635
                     /gene="rhaA"
                     /locus_tag="OO249_001126"
     CDS             31376..32635
                     /gene="rhaA"
                     /locus_tag="OO249_001126"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5635498.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWGEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32902..33726
                     /gene="rhaD"
                     /locus_tag="OO249_001127"
     CDS             32902..33726
                     /gene="rhaD"
                     /locus_tag="OO249_001127"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR5635499.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33736..>33947
                     /gene="rhaM"
                     /locus_tag="OO249_001128"
     CDS             33736..>33947
                     /gene="rhaM"
                     /locus_tag="OO249_001128"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5635500.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASA"
BASE COUNT         8323 a         8845 c         8806 g         7973 t
ORIGIN      
        1 cgccggtttt ccgctcatcg taaaatcctt ttgaaataaa taagaaccag aagcctagcg
       61 ccagtcaaca acaggaaatg ataatgagtt aacaccccgg aatttttcag aatttataaa
      121 aggcaaaatt caggcacaaa aaaagcgccg tgcggcgctt ttttcggaaa tccggtctta
      181 tttgctgccc gggatgttga aacgcttgtt gaagcggtca acacggccac cggtagcaac
      241 atcacgctgt ttgccagtga agaacgggtg gcacttgctg cacacgtcga ggttcaggtc
      301 atgaccaaca gtggagcgga ttttcattac gttaccgcaa gagcagctag cagtaatttc
      361 ttcgtatttc gggtgaatat cttttttcat gggaaaacct cggtttaagg ccgcgtcgct
      421 cttccagccc taacgccaga caccacgcga tgttaaaagt atagcttcaa tacgatcatt
      481 tcgtacgaag gcgcgaaatc atacagaaat taaccaacgt atgcaaactg atccgcactc
      541 ttctacggca atgtgtatac taacccaccg aatttcaagt caggatgatg ctatgcccgt
      601 tgcccacgtt gccttgcccg ttccgcttcc tcgtaccttt gactatctgc tgccagaagg
      661 catgacggtt aaagccgggt gtcgcgtgcg cgtgccgttt ggcaaacagc aggagcgcat
      721 cggggttgtg gtgtcagtta gcgatgtcag tgagctgccg cttaacgagc tgaaagcggt
      781 agtggaagtg ctggacgttg agccggtatt tactcactcc gtctggcggt tactgctctg
      841 ggcggcagat tactatcatc atccgattgg cgatgtgctg tttcatgcct tgccgatttt
      901 actgcgccag gggcggcctg cggcgaacgc gccgatgtgg tactggtttg ccactgaaca
      961 aggccaggcg gtggatctga acagcctgaa acgctccccc aagcaacaac aggcgctggc
     1021 ggcgttacgg caaggcaaaa tctggcgcga ccaggtcgcc acgctcgaat ttaatgatgc
     1081 cgcgttgcag gcgctacgca aaaaaggtct gtgtgattta gcaagtgaaa caccagagtt
     1141 tagcgactgg cgaacgaact atgccgtttc tggtgagcgg ttgcgattga ataccgaaca
     1201 ggccaccgcc gttggcgcaa ttcatagcgc ggcagatact ttttctgcct ggctgctggc
     1261 gggcgttacc ggttccggta aaacggaggt ttatctcagt gtactggaaa acgtgctcgc
     1321 tcagggcaaa caggcgctgg tgatggtgcc ggaaatcggc ctgacaccgc aaactatcgc
     1381 ccgttttcgt gaacgtttta atgcccccgt ggaagttctg cattccggcc tgaacgacag
     1441 cgagcggctt tctgcgtggt tgaaagcgaa aaatggcgag gcggcgattg tgatcggcac
     1501 ccgctccgcg ctgtttacgc cgtttaaaaa tctcggcgtg attgtcattg atgaagagca
     1561 cgacagctcc tacaagcagc aggaaggctg gcgctatcat gcccgcgacc tggcggtgta
     1621 tcgtgcgcac agcgagcaaa tcccgattat tcttggctcc gcaacgcccg cgctggaaac
     1681 gttatgcaac gtccagcaga aaaaataccg cctgctgcgc ctgacccgtc gggcagggaa
     1741 tgctcgtccg gcaattcaac atgtgctgga tttaaaaggt cagaaggtgc aggcaggtct
     1801 ggctccggcg ttaatcaccc gtatgcgcca gcatttacag gccaacaatc aggttatcct
     1861 ctttcttaat cgtcggggtt ttgcgcctgc gctgctgtgc cacgactgcg gctggatagc
     1921 cgaatgccca cgttgcgatc actactacac gctgcatcag gcgcaacagc atttgcgctg
     1981 tcaccactgt gacagtcagc gtccggtgcc gcgccagtgc ccttcctgcg gttccacgca
     2041 cctggtcccc gtggggctgg gcaccgaaca gcttgaacag acgcttgcgc cgatgttccc
     2101 cggcgtgccc atttcacgta tcgaccgcga taccaccagc cgcaaagggg cactggaaca
     2161 gcaactggcg gaagtgcatc gcggcggcgc gaggattttg atcggcacac aaatgctggc
     2221 gaaaggtcac cactttccgg atgtgacgct ggtggcgtta ctggatgtgg acggcgcgct
     2281 gttttctgcc gattttcgct cggcagaacg tttcgcccag ctttacaccc aggtcgccgg
     2341 tcgtgccggg cgtgcgggta aacagggcga agtggtgctg caaacgcacc atccggaaca
     2401 tcctctgttg caaacgttgc tctataaagg ctacgacgcc tttgccgaac aggcgctggc
     2461 tgagcgacgc atgatgcagc tgccgccgtg gaccagccat gtgattgtgc gtgcggaaga
     2521 tcataacaat cagcacgcgc cgctgttcct gcaacaactg cgtaatctga tcctttccag
     2581 cccgctggca gacgacaaac tgtggattct cggcccggtt ccggctctgg cacctaagcg
     2641 tggtggccgc tggcgctggc agatcttgtt gcagcaccct tcccgcgtgc gcttgcaaca
     2701 catcattagt ggtacgctgg cgcttattaa tacaataccg gattcccgca aggtgaaatg
     2761 ggtgttggat gtcgatccca tcgaaggcta atgcgcaaac gttgcgaggc ggatcgaaaa
     2821 attcaatatt catcacactt ttcatgaaaa ttctgtaacc gttttcacgc gctatctgct
     2881 aaaaatgttg ccgatgtgaa gtaaacatgg atgtagtacg cctgacgtac caggcgagga
     2941 gtgagtgtga aagcgaagaa gcaggaaact gccgcgacca tgaaagacgt tgccctcaag
     3001 gcaaaagtct ctacagcgac tgtctcccga gcattaatga atcccgataa agtctcccag
     3061 gccacccgta atcgggttga aaaagcggcc cgggaagtgg gttatttacc gcagcctatg
     3121 gggcgcaacg tcaagcgtaa tgaatcccgc accattctgg tgattgtccc ggatatctgc
     3181 gatcccttct ttagcgaaat tattcgcggt atcgaagtta cggcggcaaa tcacggatat
     3241 ctggtgctga ttggcgactg tgcgcatcaa aatcagcagg aaaaaacctt tatcgatttg
     3301 atcatcacca agcaaattga tggcatgttg ctgctgggtt caaggctgcc gtttgatgcc
     3361 agcattgagg aacagcgtaa tctgccgccg atggtgatgg cgaacgaatt tgcaccggaa
     3421 ctggagctgc ctaccgttca tatcgacaat ctgaccgccg catttgatgc agtaaattat
     3481 ttatatgagc aagggcataa acggattggc tgtatagccg gtcccgaaga gatgccactg
     3541 tgtcactacc gcctgcaagg ctatgttcag gcgctgcgtc gctgcggaat tatggttgat
     3601 ccacaataca tcgcccgtgg cgacttcacc ttcgaagccg gaagcaaagc aatgcaacag
     3661 ctgcttgatc ttccacaacc gcctactgca gtcttctgcc atagcgatgt gatggcgctc
     3721 ggcgcacttt ctcaggcaaa acgccagggg ctgaaagtcc cggaagacct ttccataatc
     3781 ggttttgata acatcgacct gacgcaattt tgtgatccgc cgctgacaac catcgcgcag
     3841 ccgcgttacg aaatcggtcg ggaagctatg ctgttattgc ttgatcaaat gcaggggcaa
     3901 cacgttggca gtggctctcg tttaatggac tgcgaactta tcatccgggg atcaacacgc
     3961 gcgttacctt aaagtaaacc tttaagactg ccgtgactgg tcaaaggccc gccgcttaag
     4021 taacatggcg ggctgacgaa cgaataaata cagcgaaacg atagtggcac aacgagatta
     4081 tgtacgccgc agccaaccgg caccttcgcg gcgaaaaaag agcacctcac ggaaaaagca
     4141 acgaaatctg cctgcggttt ctcccgctat ggtcgctatt gctgccgccg ttcttgtgac
     4201 ctttatcggt ggtctgtact tcattacgca tcacaagaaa gaagagtccg agacgctgca
     4261 aagccagaaa gtgaccggaa acggactacc accaaaacca gaagaacgct ggcgctacat
     4321 taaagagctg gaaagtcgcc agccgggtgt gcgtgcgccc acagaacctt ctgccggtgg
     4381 tgaagtgaaa acgccggagc aactgacacc agaacaacgt cagcttcttg aacaaatgca
     4441 ggctgatatg cgccagcagc caacgcagct ggttgaggtt ccgtggaatg aacagacgcc
     4501 ggaacagcgt cagcaaacgc tacaacgcca gcgtcaggcg cagcaattag cggagcagca
     4561 acgcctggct cagcagtcgc gtacgactga acaaagctgg cagcagcaga cgcgtacgtc
     4621 gcaagccgcg ccggtacagg cgcaaccgcg ccagtctaaa ccagcttcta ctcagcagcc
     4681 gtaccaggat ctgctgcaaa cccctgcgca cacgaccgcg cagtcgaagc cgcagcaagc
     4741 tgcaccggtt gctcgtgccg ctgacgcgcc aaaaccgacg gcggagaaaa aagacgaacg
     4801 ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa caggcagaga cggtacgtgc
     4861 tcagctggcg ttcgaaggat ttgactcgaa aatcaccacc aacaatggct ggaatcgtgt
     4921 ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc accctcaatc ggttgaagat
     4981 ggcgggtcat acaaactgca ttcggctcgc cgccgggggt tgaaaccctc aaaatccccc
     5041 ccatctataa ttgcattatg cctcgtactt ttgtacgggg tttgtactct gtattcgtaa
     5101 ccaaggggtc agctcgtgac aactatagta agcgtacgcc gtaacggcca tgtggtcatc
     5161 gctggtgatg gtcaggccac gttgggcaat accgtaatga aaggcaacgt gaaaaaggtc
     5221 cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg gcggtactgc ggatgctttt
     5281 acgctgttcg aactgtttga acgtaaactg gaaatgcatc agggccatct ggttaaagcc
     5341 gccgttgagc tggcaaaaga ctggcgtacc gatcgcatgc tgcgcaaact tgaagcactg
     5401 ctggcagtcg cggatgaaac tgcatcgctt atcatcaccg gtaacggtga cgtggtgcag
     5461 ccagaaaacg atcttattgc tatcggctcc ggcggccctt acgcccaggc tgcggcgcgc
     5521 gcgctgttag aaaacactga acttagcgcc cgtgaaattg ctgaaaaggc gttggatatt
     5581 gcaggcgaca tttgcatcta taccaaccat ttccacacca tcgaagaatt aagctacaaa
     5641 gcgtaaggat ctcccatgtc tgaaatgacc ccacgcgaaa tcgtcagcga actggataag
     5701 cacatcatcg gccaggacaa cgccaagcgt tctgtggcga ttgctctgcg taaccgctgg
     5761 cgtcgcatgc agctcaacga agagctgcgc catgaagtga ccccgaaaaa tatcctgatg
     5821 atcggcccga ccggtgtcgg taaaactgaa atcgcccgtc gtctggctaa gctggcgaat
     5881 gcgccgttca tcaaagttga agcgaccaaa ttcaccgaag tgggctatgt cggtaaggaa
     5941 gtggattcta ttattcgcga tctgaccgat gccgccgtga aaatggtacg cgtccaggct
     6001 atcgagaaaa accgttatcg cgctgaagaa ctggcagaag aacgtattct cgacgtgctg
     6061 atcccacctg ctaaaaacaa ctggggacag accgaacagc agcaggaacc gtccgctgct
     6121 cgtcaggcat tccgcaaaaa actgcgtgaa ggccagcttg atgacaaaga aatcgagatc
     6181 gatcttgccg cagcaccgat gggcgttgaa attatggctc ctccaggcat ggaagagatg
     6241 accagccagc tgcagtccat gttccagaac ctgggcggcc agaagcaaaa agcgcgtaag
     6301 ctgaaaatca aagacgccat gaagctgctg attgaagaag aagcggcgaa actggtgaac
     6361 ccggaagagc tgaagcaaga cgctatcgac gctgttgagc agcacgggat cgtgtttatc
     6421 gacgaaatcg acaaaatctg taagcgcggc gagtcttccg gtccggatgt ttctcgtgaa
     6481 ggcgttcagc gtgacctgct gccgctggta gaaggttgca ccgtttccac caaacacggg
     6541 atggtcaaaa ctgaccacat tctgtttatc gcttctggcg cgttccagat tgcgaaaccg
     6601 tctgacctaa tcccggaact gcaaggtcgt ctgccaatcc gcgttgaact gcaggcactg
     6661 accaccagcg acttcgagcg tattctgacc gagccgaatg cctctatcac cgtgcagtac
     6721 aaagcactga tggcgactga aggcgtaaat atcgagttta ccgactccgg tattaaacgc
     6781 atcgcggaag cggcatggca ggtgaacgaa tctaccgaaa acatcggtgc tcgtcgttta
     6841 cacactgttc tggagcgttt aatggaagag atttcctacg acgccagcga tttaagcggt
     6901 caaaatatca ctattgacgc agattatgtg agcaaacatc tggatgcgtt ggtggcagat
     6961 gaagatctga gccgttttat cctataatcg cgttcaatca ttttcatcat tgtttgatgg
     7021 ggctgaaagg ccccattttt attggcgcgt attatgactg aacaacaaat tagccgaact
     7081 caggcgtggc tggaaagttt acgacctaaa accctccccc tcgcctttgc tgcaattatc
     7141 gtcgggacgg cgctggcatg gtggcaaggt cattttgatc cgctggtcgc cctgctggca
     7201 ctgattaccg ccgggctatt acagatcctt tctaacctcg ccaatgatta cggcgatgcg
     7261 gtaaaaggca gcgatagacc tgaccgcatt gggccgctac gcggcatgca aaaaggggtc
     7321 attacccagc aagagatgaa acgggcgctc attattaccg tcgtgctcat ctgtctctcc
     7381 gggctggcac tggttgcagt ggcgtgccat acgctggctg attttgtcgg tttcctgatt
     7441 cttggcgggt tgtcgatcat tgccgctatc acctacaccg tgggcaatcg tccttatggt
     7501 tatatcggtc tgggggatat ttccgtactg gttttctttg gctggttaag cgtcatgggg
     7561 agctggtatt tacaggctca tacattgatt ccggcactga tccttccggc gaccgcatgc
     7621 ggcctgctgg caacggcagt actgaacatt aataacctgc gtgatatcaa tagcgaccga
     7681 gaaaacggca aaaacacgct ggtggtgcgc ttaggtgaag tgaacgcgcg tcgttatcat
     7741 gcctgcctgc tgatgggctc tctggtatgt ctggcgctgt ttaatctctt ttcgctgcat
     7801 agcctgtggg gctggctgtt cctgctggcg gcaccgttgc tggtgaagca agcccgttat
     7861 gtgatgcggg aaatggaccc ggtggcgatg cgaccgatgc tggaacgcac tgtcaagggg
     7921 gcgttactga ctaacctgct gtttatttta gggatattcc taagccagtg ggcagcataa
     7981 ctgacaaata tcaattaaca attgatgatt ttgccaacag cccacatagc gcgatatact
     8041 gaaaattctc gcagcaactg aatgttaagc ctatgaaata cgatacttcc gagctttgtg
     8101 acatctatca agaagatgtt aacgtcgtgg aaccgctgtt ctccaacttt ggcggacggg
     8161 cgtcgtttgg cggacaaata atcacggtaa aatgtttcga ggacaacggg ttgctgtacg
     8221 atctgctcga acagaatggc cgtggtcgtg ttcttgtcgt cgatggcggt ggttctgttc
     8281 gtcgcgcact ggtcgatgct gaactggcgc gtctggcagt acaaaatgaa tgggaaggtc
     8341 tggttattta cggcgcggtg cgtcaggtag atgacctgga agagttggat atcggcatcc
     8401 aggcgatggc ggcaattccg gttggtgccg ctggcgaagg cattggcgaa agcgatgtcc
     8461 gcgtcaattt tggcggtgta accttcttct ccggcgacca tctttatgcc gacaataccg
     8521 ggattattct ttcagaagat ccgctggata ttgaatgata agaaaggcac cgcaaggtgc
     8581 cttttttctg cgttacctgt tggcctacac agtaaagaaa ttacgcgaaa gatgaagcgt
     8641 aatcagacct cttccatgcg acccagcagg gcctgcagac gttcctgcca gccgttctgc
     8701 tgttctttca gatggttgtt ctcacgctcc agctcttcgc gctgatgctg ggcattttga
     8761 acttcctgcg acagtgagtt gtttttttct ttcagctctt cgatttccat ctgcaacaga
     8821 gtgatggtat caatcgcctg ctgtactttt gcttccagtt tctcaaacac ttctaatgac
     8881 attgtcatac ctctcctgaa ttgcaaggcg ttgatggata aaaaatcctc gtcccgatta
     8941 ccggtgacgc cttaataaat acgagcgcac tttagttagc tccgattgta tgaagccgcg
     9001 ccatcgctgt ccagcggcac gccttgcaga ttacggtttg ccacactttt catccttctc
     9061 ctggtgacat aatccacatc aatcgaaaat gttaataaat ttgttgcgcg aatgatctaa
     9121 caaacatgca tcatgtacaa tcagatggaa taaatggcgc gataacgctc attttatgac
     9181 gaagcacaca cattttaagt tcgatatttc tcgtttttgc tcgttaacga taagtttaca
     9241 gcatgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa cattaactct
     9301 tcaggatccg attatgagtc aaacatcaac cttgaaaggc cagtgcattg ctgaattcct
     9361 cggtaccggg ttgttgattt ttttcggtgt gggttgcgtt gcagcactaa aagtcgctgg
     9421 tgcgtctttt ggtcagtggg aaatcagtgt catttgggga ctgggggtgg caatggctat
     9481 ctacctgacc gcaggggttt ccggcgcgca tcttaatccc gctgttacca ttgcattgtg
     9541 gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt atcgtttcac aagttgccgg
     9601 cgctttctgt gctgcggctt tagtttacgg gctttactac aatttatttt tcgacttcga
     9661 gcagactcat cacattgttc gcggcagcgt tgaaagtgtt gatctggcag gcactttctc
     9721 tacttaccct aatcctcata tcaattttgt gcaggctttc gcagttgaga tggtgattac
     9781 cgctattctg atggggctga tcctggcgtt aacggacgat ggcaacggtg taccacgcgg
     9841 ccctttggct cccttgctga ttggtctact gattgcggtc attggcgcat ctatgggccc
     9901 attgacgggt tttgccatga acccagcgcg tgacttcggt ccgaaagtct ttgcctggct
     9961 ggcgggctgg ggcaatgtcg cctttaccgg cggcagagac attccttact tcctggtgcc
    10021 gctttttggc cctatcgttg gcgcgattgt aggtgcattt gcctaccgca aactgattgg
    10081 tcgccatttg ccttgcgata tctgtgttgt gaaagaaaag gaaaccacaa ctccttcaga
    10141 acaaaaagct tcgctgtaat atgactacgg gacaattaaa catgactgaa aaaaaatata
    10201 tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat cacgatgcca
    10261 atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca ggttgggtag
    10321 aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa gtgctggcga
    10381 aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag cgtgaaacca
    10441 ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc tggcagtgcc
    10501 gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat tatatccgca
    10561 gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag tggatcctcg
    10621 accatgtgga aggctctcgc gagcgtgcac gtcgtagtga attgctgttt ggtacggttg
    10681 atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat tacaccaacg
    10741 cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa atgctggaag
    10801 tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa gtatacggtc
    10861 agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc gccggtgacc
    10921 agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag aacacctatg
    10981 gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca gaaaacggcc
    11041 tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg gaaggtgcgg
    11101 tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg attaacgacg
    11161 cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg tatgtggttc
    11221 cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg gcgattttcg
    11281 gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag tctattgctt
    11341 atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt ctgcacgccc
    11401 tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag tccgatattc
    11461 tcggcacccg tgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt gcggcctatc
    11521 tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag aaagcggtga
    11581 ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt tacgcaggct
    11641 ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa tgtaaatgcc
    11701 ggatgaagcg tttatgccgc atccggtagt cctgaaacgt gcgggggcaa ccccgcacac
    11761 atcaataatc ccacccttcc cctgtgctac acttcgcgcc attccttact gcttagagtt
    11821 tgctatgaga cgagaacttg ccatcgaatt ttcccgcgtc accgaatctg cggcgctggc
    11881 tggctacaaa tggttaggac gcggcgataa aaacaccgcg gacggcgcgg cggtaaacgc
    11941 catgcgtatt atgctcaacc aggtcaacat tgacggcacc atcgtcattg gtgaaggtga
    12001 aatcgacgaa gcaccgatgc tctacattgg tgaaaaagtc ggtactggtc gcggcgacgc
    12061 ggtagatatt gctgttgatc cgattgaagg cacgcgcatg acggcgatgg gccaggctaa
    12121 cgcgctggcg gtgctggcag taggcgataa aggctgcttc ctcaatgcgc cggatatgta
    12181 tatggagaag ctgattgtcg ggccgggagc caaaggcacc attgatctga acctgccgct
    12241 ggcggataac ctgcgcaatg tagcggcggc gctcggcaaa ccgttgagcg aactgacggt
    12301 aacgattcta gctaaaccac gccacgatgc cgttatcgct gaaatgcagc aactcggcgt
    12361 acgcgtattt gctattccgg atggcgacgt tgcggcctca attctcacct gtatgccaga
    12421 cagcgaagtt gacgtgctgt acggtattgg tggcgcgccg gaaggcgtag tttctgcggc
    12481 ggtgatccgc gcattagatg gcgacatgaa cggtcgtctg ctggcgcgtc atgacgtcaa
    12541 aggcgacaac gaagagaatc gtcgcattgg cgagcaggag ctggcacgct gcaaagcgat
    12601 gggcatcgaa gccggtaaag tattgcgcct gggcgatatg gcgcgcagcg ataacgtcat
    12661 cttctctgcc accggtatta ccaaaggcga tctgctggaa ggcattagcc gcaaaggcaa
    12721 tatcgcgact accgaaacgc tgctgatccg cggcaagtca cgcaccattc gccgcattca
    12781 gtccatccac tatctggatc gcaaagaccc ggaaatgcag gtgcacatcc tctgattgat
    12841 ttgatcgatt gagccttcca gtccttcggg actggaattt ttttgttcgg agaacgaaga
    12901 taaggcaagt caatcaaaac aggagaaaaa catggctgat tgggtaacag gcaaagtcac
    12961 taaagtgcag aactggaccg acgccctgtt tagtctcacc gttcacgccc ccgtgcttcc
    13021 gtttaccgcc gggcaattca ccaagcttgg ccttgaaatc ggcggcgaac gcgtccagcg
    13081 cgcctactcc tatgttaact cgcccgataa tcccgatctg gagttttacc tggtcaccgt
    13141 ccccgatggc aaattaagcc cacgattggc ggcactgaaa ccaggcgatg aagtgcaggt
    13201 ggttagcgaa gcagctggct tctttgttct ggatgaagta ccagattgcg aaacgctatg
    13261 gatgctggca accggtacag cgattggccc ttatttatcg attctgcaac taggcaaaga
    13321 tttagatcgc ttcaaaaatc tggtcctggt tcacgccgca cgttatgccg ccgacttaag
    13381 ctatttgcca ctgatgcaag aactggaaaa acgctacgaa ggcaaactgc gcattcagac
    13441 ggtggtcagt cgggaaacgg cagcggggtc gctcaccgga cggataccgg cattaattga
    13501 aagtggggaa ctggaaagcg cgattggcct gccgatgaat aaagaaacca gccatgtgat
    13561 gctgtgcggc aatccacaga tggtgcgcga tacacaacag ttgctgaaag agacccggca
    13621 gatgacgaaa catttacgtc gccgaccggg ccatatgaca gcggagcatt actggtaagc
    13681 ggttacttat cgataaacgg cacgatgagc aaatccgcac tcatcttatt gatcatcccg
    13741 cgatatgctg gcatcaaacg gttgataaat gagtgatgat gaccacagac aaggaggtcg
    13801 cactgctctt tttgcataat ttccagcagt gtttccggca tttctccgcg ttcaatacgc
    13861 agttttgtct tcggccattg aatatttttc gtcagtttat acagcttgtt atccgactta
    13921 ttcttcaaca attgaagaat atcttctgtt gcagggaagt agatacccgg atacaactcg
    13981 cttaagccat catcaatatg aattaacgtc aggtgagcgt cattatgtct ggcgagctcc
    14041 agagctttat tcaccagtaa ggcatcttct tcattcccgg aaattgccac gccaatgtgt
    14101 ttataagcca tgtttaactc cttctaaagc aactccatca agctaacgaa cgcagtgata
    14161 actcaaaaat aatcatctcg gcctggcatg agaaagtgaa ggccgcatca agctcaactt
    14221 gcccctccag atccttaatc ttgtaggcca cgctggcccc ttcatctaca gaggatgcag
    14281 cggcggcaag ctctttcagt ttggtcataa aagactctgc ttcttcgcgg gtggcaaaaa
    14341 agcgcgaaaa cttactggta cagttgtcgt tatcaatcac cgtaccgata tcaatcgcac
    14401 atcctttagt actgcattta tctacgacat ctttcatagg gcacctctgt atattcgcct
    14461 ctctgtcgat tacgacttaa gaataatgcg ccgttaaggg aagaacaaac aagtaatgat
    14521 cttatggatt accatttttt ttgatattgc ttaaggtgaa gagttcagaa ggattttccg
    14581 ggtattgtca tttgcatcgt ggaaataaca atcccggaaa aaaatagcag taagagatat
    14641 attaaaggtt aattacttga tttattgtcg gctttatact tcacatcctg agtatcttta
    14701 ccatatttat tttcgccttg tgtgccaaca aacgcaccaa gatcgataag catcatcacc
    14761 agaatcaacg tcgggacaaa acaccccacc gcccattgcc agacacccgg taaaatcgcc
    14821 cagttacccg ccagcagcat ccacgccaca atcatcagaa atgcccatgc gccggaacgc
    14881 ccgcgatcat gcaagcgctt aacagttact gccgctgttg gccaaagcaa gcacacaagg
    14941 caaaacgccg cggtctgaat atcgagtaaa tttttacccg ccagtgagaa aagcaccagc
    15001 atgcctgcga accacaggcc tatccaaatc cagaaatcac ggcgtccaat acgcccttta
    15061 aatgagaata accattgctg tatggtcatg taagttcctt gatggttgtc ttttccagga
    15121 ttctaccctt ttgacaaggg cgacagatat cgttttaatc ggagccagtc ataacaaaag
    15181 gtactgtcaa tgaagccagg gtgtacgctg ttttttctct tatgttctgc attaaccgtt
    15241 acaacaacgg cgcatgcaca aacaccagat acggcaacga ccgcgcctta tctgctggct
    15301 ggagccccta ctttcgatct ctccatcagc cagtttcgag aagactttaa cagccagaat
    15361 cctagcctgc cactgaacga atttcgtgcc atcgacagca gtcccgacaa agccaatctc
    15421 actcgtgctg caagtaaaat taacgagaac ttgtatgctt ctacagcgct ggagcgcggt
    15481 accttaaaaa tcaaaagcat tcaaatgacc tggctaccca tccaggggcc agagcaaaaa
    15541 gcagcgaaag cgaaagctca ggaatacatg gcagcagtga tccgcacact caccccatta
    15601 atgaccaaaa cacaaagcca gaaaaaactg cagtcgctgc taacggcggg gaaaaacaaa
    15661 cgttattaca ccgagacaga aggtgcactg cgttatgttg tcgcggacaa cggcgaaaag
    15721 gggctgacct tcgctgttga accgattaag ctggcgctat ctgaatcgct tgaaggtttg
    15781 aataaatgac aaaaagcaaa gcctttgtgc cgatgaatct ctatactgtt tcacagacct
    15841 gctgccctgc ggggcggcca tcttccttta ttcgcttata agcgtggaga attaaaatgc
    15901 gacatccttt agtgatgggt aactggaaac tgaacggcag ccgccacatg gttcacgagc
    15961 tggtttctaa cctgcgtaaa gagctggcag gtgttgctgg ctgtgcggtt gcaatcgcac
    16021 caccggaaat gtacatcgat atggcgaagc gcgaagctga aggcagccac atcatgctgg
    16081 gtgcgcaaaa cgtggacctg aacctgtccg gcgcattcac cggtgaaacc tctgctgcta
    16141 tgctgaaaga catcggcgca cagtacatca tcatcggcca ctctgaacgt cgtacttacc
    16201 acaaagagtc tgacgaactg atcgcgaaaa aattcgcggt gctgaaagag catggtctga
    16261 ctccggttct gtgcatcggt gaaaccgaag ctgaaaacga agcgggcaaa actgaagaag
    16321 tttgcgcacg tcagatcgac gcggtactga aaactcaggg tgctgcggca ttcgaaggtg
    16381 cggttatcgc ttacgaacct gtatgggcaa tcggtactgg caaatctgca actccggctc
    16441 aggcacaggc tgttcacaaa ttcatccgtg accacatcgc taaagttgac gctaacatcg
    16501 ctgaacaagt gatcattcag tacggcggct ctgtaaacgc gtctaacgct gcagaactgt
    16561 ttgctcagcc agacatcgac ggcgcgctgg ttggcggtgc gtctctgaaa gctgacgctt
    16621 tcgcagtaat cgttaaagct gcagaagcgg ctaaacaggc ttaagtctga caggtgccgg
    16681 atttcatatc cggcacttac tttccttaac tcttcgcctt aacgcaaaat ctcacactga
    16741 tgatcctgaa tttcctcggc tgaagcacgg ttaagcgtca gtagatttcg ttgtgtcgcc
    16801 agcaatacaa atgagttatc actctgccgt accatcgcca gcccgtagct tcccatatgt
    16861 tcccgcgcct caggtacttc ttctgccagc atcataaatg ggctgcgttg taccagttcg
    16921 ctttccgtta cccgacgcgc caggtattca tgcccgcgca aaccacctgg cagtggcaac
    16981 cagcggctgc tgatgttcgc cagattgtta tccagttgtt cgcgcacatc aggacgaata
    17041 caagagatat gaatatgaaa atggttttgc gtacgcccgg tgcgggagtt gatcgccaaa
    17101 gaaaccgcgc gatcgggaac cggctggccg tactttttgc tcataaaatc acgcgcctgc
    17161 caggccaacc aaaagaagtt cggcgttgaa ggatcggtca acaaaggact ttcagtaccg
    17221 ttaatacgat acgttggcat caacagatat tgcagtgggc cattaagatc ttttaaaacc
    17281 acgtatccgg cattgggttt gacttccgca catggcgaag gattttgatt ttgctgctga
    17341 ttgggcaaac attcctcaag gacaatctta cgtaatgtat ccgactcttc accggttaat
    17401 ttccagtaac caataccggc agccacaacg gcgataacta tcatcaccaa aaaaagaaga
    17461 cccgcttttt tcatcttttt ttccctgtac ctcaaagaga tgcaagggta acgcaaaatc
    17521 gtgacaaata aaaaacccgg tcgcgagaaa ccaccgggtt tgataattat cctggggaaa
    17581 tcagcgtttg ctgatctgat cgaacgtacc gccgttagca aaatgctctt tttgcgcttt
    17641 cgtccagccg ccgaactctt catcaatggt gaataacttc agctttggaa acgcattttc
    17701 gtactttttc gctacttcag cgtcgcgcgg acggtagtag tttttcgcgg caatttcctg
    17761 accttctggc gagtagagat atttcaggta ggcttccgcc acctctttgg tgcctttttt
    17821 ctcgaccact ttatcgacca ccgacacggt tggctctgcg aggatagact cactcggcgt
    17881 gacgatttcg aatttatctt tccccagttc attcgctgcc agcagagctt cgttttccca
    17941 ggcgatcagt acatcgccaa tcccgcgctc gacaaaggtg ttagtggagc cgcgcgcgcc
    18001 agaatccaga acttcgacgt ttttatacag tgcccgaaca aaatcctgtg cttttgcctg
    18061 atcgttgttg ttgtgatgca gcgcgtagcc ccaggcagcc aggtagttcc agcgtgcgcc
    18121 accagagctt ttcggattag gcgtgatcac cgaaacaccc ggtttaatca gatcgttcca
    18181 gtcatggatc tgcttcggat tgcccttacg caccaggaaa acaatggtgg aagtgtacgg
    18241 tgcggagtta tccggcagac gtttgatcca ctctttatca atccgcccgc gttccgcaat
    18301 tgcgtccacg tcataggcca gagccagcgt gacaacatca gcttcaatac cgttgattac
    18361 cgacgtcgct tgtttacctg aaccaccgtg tgactgacgc atcaccacgt tatcaccagt
    18421 ttgctgtttc cagtgggcgc tgaatgcctt gttgtactgt tcgtacaatt cgcgcgttgg
    18481 atcatatgaa acgttaagaa gctgaatatc ctttgccata acgctggttg ccgccagcaa
    18541 aaatgttaac cctacgcccc acttgttcat cgcccgactc tcttatgttg tgttgtgatg
    18601 agcaaagcgt gccagaaggt taaccaaaca ttaaagaata aaaaaagatt ggctataact
    18661 tgcgggtata tgttgaggga ttaaaaaggc ggaagaaatc cgcctcatat tgctgacaaa
    18721 gtgcgctttg tccatgccgg atgcggcgcg aacgccttat ccggcctaca aaagtttgca
    18781 aattcaataa attgcagaat tcatgtaggc ctgataagcg aagcgcatca ggcatttttg
    18841 cttctgtcat cggtttcagg ctaaaggaat ctgccttttt ccgaaatcat taatacagtt
    18901 ttttcgcgca gtccagccag tcacctttga acggacgctt catgttttcg atagcgtcga
    18961 tgatgtcgtg gtgaaccagc tgttcgttct ggatacctac acaacgaccg ccgtaacctg
    19021 ccagcagcag atcgatagcg taagcgccca tacgggaagc cagaatacgg tcgtaaggca
    19081 ccggagaacc accgcgctgg atgtggccca gcacagttgc gcgggtttca cgaccggttt
    19141 ctttctcgat gaaatgcgcc agttcgtcaa catcacacat atgttcggta atcgccacga
    19201 tcgcgtgttt tttacctttc gcgatacccg ctttgatttc gtttaccagg tcttcacggc
    19261 tgaattcaac ttccggaacc acaacgaatt cacagccacc ggcaatggcc gcagccaacg
    19321 tcagatcgcc acaataacgg cccatcactt ccaccacgga aatacgctgg tgagaagaag
    19381 aggtgtcacg cagacggtcg atcgcttcta caacggtgct cagcgcagtg aagaaaccga
    19441 tagtgtagtc agtgcctttg atgtcgttgt cgatagtgcc cggcagaccg atgcacggga
    19501 agcccatttc ggtcagacgc attgcaccca tgtaggaacc gtcaccgccg ataaccacca
    19561 gcgcgtcgat accacgtttt ttcaggtttt cgatagccac ggcgcggatg ttctcgtcgc
    19621 ggaattccgg gaaacgcgca gaaccgagga acgtaccgcc acggttgatc atgtcagaca
    19681 cgctgtaacg gtctagctgt accatacggt cttcatacag acccagatag ccgtcataaa
    19741 tacccattac ttccagacct tctgtcagcg cagaacgaac aaccccgcga attgcggcgt
    19801 tcatgcctgg cgcatcaccg ccgcttgtca acacaccgat tttcttaatc atgactacct
    19861 ctgaactttg gaatgcaaaa tgaaatctgt tgccggaagt cttcttgcac atcgaagtga
    19921 tccaacgaat gtgcaaatag tataacaatc acttcctgct gaattgattc aggtcaggcc
    19981 aaatggcggt attttataca caaaatgcgg gtctggctct cttttatact gattatgaaa
    20041 gcatagaccg tttaccctcc ctgggtacga cggaacaggg gtcctgatgg ataattacat
    20101 ccgatcccgg aaaacgccgt aaaatagcct gctctacctg atccgccacc atatgtgcct
    20161 gaaccaaagg cagagagtct tccatttcca aatgaatctg aataaagcgg gtcggccctg
    20221 actgccgcgt gcgaagatcg tgagcgccgc taacacccgg ccaggaagtc acgatatcaa
    20281 taatttcttg ccgttcctca tcaggcaatg cgcgatccag taatgactgt accgcctcat
    20341 atcccatgcg taacgcgcta tataaaatat agatgccgat tcccaatgca aacagagcat
    20401 cggcgcgatg ccagccgtac caggacaacc ccagcgccag cagaattgcg ccgttcatca
    20461 taacatcaga ctggtaatgt agcatatcag cccgcaccgc ctggctttgc gtccggcgca
    20521 ccacccaacg ctgaaacgag acaaggataa tcgtacaaat tagcgccaca attgtcacga
    20581 taaccccgac gcctggatct atcatcggtg ttggagatat cagatgttga atacccgtca
    20641 aaaacaggaa tagtgccgaa ccggagataa acatactttg cgccagcgcc gcgagggact
    20701 ctgctttacc gtgaccaaac gagtgattat cgtcggcagg ttgcagggaa tatcgcacca
    20761 ccagtaaatt cgtcaacgac gcgccgatat ccaccagcga atccaccagc gcggcgagaa
    20821 tactcaccga cccggtatac caccatgcaa aaattttaat cagcaatagc aacgaagcca
    20881 tcgccgtcgc agcaatcgcc gcccgactga ccagccgtcc ataagattga ttcataaata
    20941 ctcccgctat caactgacgc tagtataacg gaagcaaatc atctgcaatg cattaagcag
    21001 caggcaaatt gaggataaaa aaaaccccac atcatgtggg ggaagacagg gatggtgtct
    21061 atggcaagga aaacagggtt tactactggg aacgtgagtt gctactactc aatagcttca
    21121 acgatgaact tttttgccat tgcgtcacgt cacgcaactg ctccattcgt tgttgatgtt
    21181 tctcgtttaa aaccgcttgc tgctccggcg ttaacaggcg atacatttgg ttgcggactt
    21241 ttgccatctc aacctgacga gcaatttgct cattcgccat tttttctgcc tgtgcgcgca
    21301 cagcgttttc atcaaaattt tctgcggtga caaggcgatg cattgtctcc agttcgctaa
    21361 cattaacagg aggctgttcg tgccgggcct gttgcataag atctcgcatc tgctgacgct
    21421 gatgttcggt taaacttatg ccgtcgaaca tatggctctg cgtactgcgc tgcgtactgc
    21481 gctgcgtaag ttcttcaccc ggatgccagt tatcgcctga accgacttca gcagcgtggc
    21541 ttaatgaact gactgccagc gttgaagcca tgacggcagc ggtaactatg cgcatcattt
    21601 gctcccaaaa tctttctgtc gcgattcaac gatagagagt ttacgattca ggctgcaaac
    21661 atgcgtcagg gggtgtaaaa caacgtaaag tcatggatta gcgacgtctg atgacgtaat
    21721 ttctgcctcg gaggtattta aacaatgaat aaaatcctgt tagttgatga tgaccgagag
    21781 ctgacttccc tattaaagga gctgctcgag atggaaggct tcaacgtgat tgttgcccac
    21841 gatggggaac aggcgcttga tcttctggac gacagcattg atttactttt gcttgacgta
    21901 atgatgccga agaaaaatgg tatcgacaca ttaaaagcac ttcgccagac acaccagacg
    21961 cctgtcatta tgttgacggc gcgcggcagt gaacttgatc gcgttctcgg ccttgagctg
    22021 ggcgcagatg actatctccc gaaaccgttt aatgatcgtg agctggtggc acgtattcgc
    22081 gcgatcctgc gccgttcgca ctggagcgag caacagcaaa acaacgacaa cggttcaccg
    22141 acactggaag ttgatgcctt agtgctgaat ccaggccgtc aggaagccag cttcgacggg
    22201 caaacgctgg agttaaccgg tactgagttt accctgctct atttgctggc acagcatctg
    22261 ggtcaggtgg tttcccgtga acatttaagc caggaagtgt tgggcaaacg cctgacgcct
    22321 ttcgaccgcg ctattgatat gcacatttcc aacctgcgtc gtaaactgcc ggatcgtaaa
    22381 gatggtcacc cgtggtttaa aaccttgcgt ggtcgcggct atctgatggt ttctgcttca
    22441 tgataggcag cttaaccgcg cgcatcttcg ccatcttctg gctgacgctg gcgctggtgt
    22501 tgatgttggt tttgatgtta cccaagctcg attcacgcca gatgaccgag cttctggata
    22561 gcgaacagcg tcaggggctg atgattgagc agcatgtcga agcggagctg gcgaacgatc
    22621 cgcccaacga tttaatgtgg tggcggcgtc tgttccgggc gattgataag tgggcaccgc
    22681 caggacagcg tttgttattg gtgaccaccg aaggccgcgt gatcggcgct gaacgcagcg
    22741 aaatgcagat cattcgtaac tttattggtc aggccgataa cgccgatcat ccgcagaaga
    22801 aaaagtatgg ccgcgtggaa ctggtcggtc cgttctccgt gcgtgatggc gaagataatt
    22861 accaacttta tctgattcgt ccggccagca gttctcaatc cgatttcatt aacttactgt
    22921 ttgaccgccc gctattactg ctgattgtca ccatgttggt cagtacgccg ctgctgttgt
    22981 ggttggcctg gagtctggca aaaccggcgc gtaagctgaa aaacgctgcc gatgaagttg
    23041 cccagggaaa cttacgccag cacccggaac tggaagcggg gccacaggaa ttccttgccg
    23101 caggtgccag ttttaaccag atggtcaccg cgctggagcg catgatgacc tctcagcagc
    23161 gtctgctttc tgatatctct cacgagctgc gcaccccgct gacgcgtctg caactgggta
    23221 cggcattact gcgccgtcgt agcggtgaaa gcaaggaact ggagcgtatt gaaaccgaag
    23281 cgcaacgtct ggacagcatg atcaacgatc tgttggtgat gtcacgtaat cagcaaaaaa
    23341 acgcgctggt tagcgaaacc atcaaagcca accagttgtg gagtgaagtg ctggataacg
    23401 cggcgttcga agccgagcaa atgggcaagt cgttgacagt taacttcccg cctgggccgt
    23461 ggccgctgta cggcaatccg aacgccctgg aaagtgcgct ggaaaacatt gttcgtaatg
    23521 ctctgcgtta ttcccatacg aagattgaag tgggctttgc ggtagataaa gacggtatca
    23581 ccattacggt ggacgacgat ggtcctggcg ttagcccgga agatcgcgaa cagattttcc
    23641 gtccgttcta tcggaccgat gaagcacgcg atcgtgaatc tggcggtaca ggattggggc
    23701 tggcgattgt tgaaaccgcc attcagcagc atcgtggctg ggtgaaggca gaagacagcc
    23761 cgctgggcgg tttacggctg gtgatttggt tgccgctgta taagcggagt taaactccgc
    23821 atttgtaggc aggataaggc gtttacgccg catccggcat ttgagcagga tgcctgatgc
    23881 gacgctgata gcgtcttatc aggcctacac tccgggggtt attttcccca caaccggcgg
    23941 gaaaaatctt cgatcttgcc gctcagtcgg cgcttttgca tcgtccgcgt ccagcttttc
    24001 gataacccgg cggcggagag taaacggtga tactgatcgt catcaaacgg catatgccat
    24061 gcgatggcgg cagcctcctg cacggtcaca tcgctgacac gggaaaccaa ctccagcggc
    24121 gcgtccgcag acactttccc tggcgctatc acgctataca accaccccac cttacctgtg
    24181 ttttgcatca actgcgctat atcgctgata tcaaaatggt aattgagttt atagcaaggc
    24241 gagcgcggct ggctgacctg aattaatgcc tctccccagc ggaaaatatc gcccatataa
    24301 acattacttt ccgtcaggcc gtcggttgag aggttttcac caaacgcagg cgcaacaaac
    24361 aactccgcct gttccggaaa ttcccgcgcc cagtagagat aatgctcacg aggataatga
    24421 cacagcgctc tgtctggccc accatgaacc tttttctccg cctgctcgtc accttccagc
    24481 cccagctccg tcagcatcaa ctcaccatca acctggattt tggcaattgc gctgggtttg
    24541 ccttcgggat aagcctgaat cttgcctgtg tatacatcaa ccggatatcg catctgttcc
    24601 ctctccacga gaaatttccc cattagacca tatttatttt atgtcaggcg tgctgttttc
    24661 gcggtaagta tccaggcagg aactcaatag caaaccggac gaacgttagt tcatccggct
    24721 gtggggatta aggaagcagg tttatttcac cgtacctaaa atttcgattt tcgctgctct
    24781 ggctttgact ttacgtgacc agatagaggt aagaattggc accagaatcg aagtcacaat
    24841 gaccgccgtc gctaccagcg aagttgctgc cggagccatc ggtttaaacg caggcaccat
    24901 ttctgcaatc agcataggtg tcgctaccgc ggcccctgcg gaactggaag cggcaattcc
    24961 ggcagtgcca tcgccaccgc caatcaattt atcggcgata atcaacggaa taccggtcac
    25021 gataattact gccacaccca acaggatccc cagcaaacca gtctgggcaa tcacagtcaa
    25081 atcaatggta ttgcccagcg cgaaggcaaa gaatggaatc agcgtttgca ccgctttgct
    25141 gaaaaattct cgtaattcag ggtcaaggtt ccccagggca aagcccacca ggaacggtaa
    25201 tactgcgccg acgaaaacat gcggttcaaa cgaggcaatc cctgcagtgc ccagaataat
    25261 catcgtcatg agcggcccgg actccaacga catcaacaca aatgccccag cttcttcttt
    25321 tgtgccgtac tgctgcatga tggaagcgta aagtccgcca ttggtcatat ccatcgccgc
    25381 caccagcgcc agcgttgaaa gtccggcaaa gaatccaact tcaacaccat gttccggaat
    25441 aatgcgcgag gcaattgccg caaccaccca cgcgacggca atttttgtta ccaccagcgt
    25501 accggattta cgcaataccg ttcccgtcgc gcttaatttt attgacgccc ccatgcaaaa
    25561 aaaccacacc gccagaatgg acaccgtacc ggtaatcatc ccgttggtga atgatccaaa
    25621 atatttcccc gcccccggcg agaaggtgtg gcacagtgcg ccaaggaata gcgggacgag
    25681 catcatcccc ccccgggatt ttctcaatcg agcgttttat ctgcatttcc atcacctgtc
    25741 ggcatatttt taagggctga tgcgtaattc ctcaatggag catcagtgtt gatggaaacg
    25801 aagatagaca ttcatttaat caataaaagt gatcaaaacc acatcaattg aaacgctgtt
    25861 ttatttttat cggatcattg tttttatttt gaaattgatc acaaaaaaac caccgccgtt
    25921 ggcgatggtt cattttttaa gctgatatgc ggcgtaaacg cctcattgca gcaggcggca
    25981 aatgattatt ttttcgccgc gaaacgtgcc gctgcttcat cccagttcac cacgttccag
    26041 aactctttga tgtagtccgg acggcggttc tggaatttca ggtagtaagc atgttcccac
    26101 acatccaggc ccagaatcgg gaagccggaa gcgccagaaa tagcttcacc catcagcggg
    26161 gaatcctggt tagcagtaga aaccaccgcc agtttatcgc ctttcagcac cagccatgcc
    26221 cagccggaac caaagcggga agctgccgct ttctcaaatt ctgctttgaa gttatcaacg
    26281 gagccgaagt cacgttcgat agccgctttc aggtcaccct gtaaggtggt gccttttttc
    26341 agacctttcc agaacaggct gtggttagcg tgaccgccag cgttgttgcg cagtacggtt
    26401 ttcttgtctg ctggcagctg gtccagtttg gtgatcagct cttcaaccgg caggttggca
    26461 aattctggca ggctttccag cgccgcgttg gcgttgttga cgtaggtctg atggtgtttg
    26521 gtgtggtgga tttccatggt ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac
    26581 ggcagggatg gcagggtata gctcatattc atctccagta ttgtcgggcg gccgattgtt
    26641 aatgcgcgta agcagttggt tcattatagt taattaaatg atattgaaaa tgattatcaa
    26701 tgccgtactt ttcgtaaggg tatggttttg caggaaaatt cccgagatgt gaagcaaatc
    26761 acccacttaa tgccgtgatt gccagtaaat cgacaacggc ggcaacaggc gaaaggttaa
    26821 tcgacagcac gatttttaca ctcatctcgt cggagatgtg acgcgacgaa aaatgatgag
    26881 gataagaaga tgagtaacgc gattacgatg gggatatttt ggcatttgat cggcgcggcc
    26941 agtgcagcct gtttttacgc tccgttcaaa aaagtaaaaa aatggtcatg ggaaaccatg
    27001 tggtcagttg gtgggattgt ttcgtggatt attctgccgt gggccatcag cgccctgtta
    27061 ctgccgaatt tctgggcgta ttacagctcg tttagtctct ctacgctgct gcctgttttt
    27121 ctgttcggcg ctatgtgggg gatcggtaat atcaactacg gcctgaccat gcgttatctc
    27181 ggcatgtcga tgggaattgg catcgccatt ggcattacgt tgattgtcgg tacgctgatg
    27241 acgccaatta tcaacggcaa tttcgatgtt ctgcttaata ccgaaggcgg acgcatgacg
    27301 ttgctcggcg ttctggtggc gctgattggt gtagggattg tgactcgcgc cgggcagttg
    27361 aaagagcgca aaatgggcat taaagccgaa gagttcaacc tgaaaaaagg gctggtgctg
    27421 gcggtgatgt gcggcatttt ctctgccggt atgtcttttg cgatgaacgc cgcaaaaccg
    27481 atgcatgaag ccgctgccgc actgggcgtc gatccactgt atgtcgctct gccaagctat
    27541 gtcatcatca tgggcggcgg cgcgatcatc aacctcggtt tctgcttcat tcgtctggca
    27601 aaagtgaagg atttgtcgct aaaagccgac ttctcgctgg caaaaccgct gatcattcac
    27661 aatgtgttac tctcagcact gggcgggttg atgtggtatc tgcaattctt tttctatgcc
    27721 tggggccacg cccgcattcc ggcgcagtat gactacatca gttggatgct gcatatgagt
    27781 ttctatgtat tgtgcggcgg tatcgtcggg ctggtgctga aagagtggaa caatgcaggt
    27841 cgccgtccgg taacggtgtt gagcctcggt tgtgtggtga ttattgtcgc cgccaacatc
    27901 gtcggcatcg gcatggcgaa ttaatctttc tgcgaattga gatgacgcca ctggctgggc
    27961 gtcatcccgg tttcccgggt aaacaccacc gaaaaatagt tactatcttc aaagccacat
    28021 tcggtcgaaa tatcactgat taacaggcgg ctatgctgga gaagatattg cgcatgacac
    28081 actctgacct gccgcagata ttgattgatg gtcattccag tctgctggcg aaattgctga
    28141 cgcaaaacgc gctcactgca cgatgcctca tcacaaaatt tatccagcgc aaagggactt
    28201 ttcaggctag ccgccagccg ggtaatcagc ttatccagca acgtttcgct ggatgttggc
    28261 ggcaacgaat cactggtgta acgatggcga ttcagcaaca tcaccaactg cccgaacagc
    28321 aactcagcca tttcgttagc aaacgacaca tgctgactac tttcatgctc aagctgaccg
    28381 ataacctgcc gcgcctgcgc catccccacg ctacctaagc gccagtgtgg ttgccctgcg
    28441 ctggcgctaa atcccggaat cgctccctgc cagtcaagat tcagcttcag ccgctccggg
    28501 caataaataa tattctgcaa aaccagatcg ttaacggaag cgtaggagtg tttatcgtca
    28561 gcatgaatgt aaaagagatc gccacgggta atgcgataag ggcgatcgtt gagtacatgc
    28621 agaccattac cgcgccagac aatcaccagc tcacaaaaat catgtgtatg ttcggcaaaa
    28681 acatcttgcg gataacggtc agccacagcg actgcctgct ggtcgctggc aaaaaaatca
    28741 tctttgagaa gttttaactg atgcgccacc gtggctacct cggccagaga acgaagttga
    28801 ttattcgcaa tatggcgtac aaatacgttg agaagattcg cgttattgca gaaagccatc
    28861 ccgtccctgg cgaatatcac gcggtgacca gttaaactct cggcgaaaaa gcgtcgaaaa
    28921 gtggttactg tcgctgaatc cgcagcgata ggcgatgtca gtaacgctgg cctcgctgtg
    28981 acgtagcaga tgtcgggcct tcatcagtcg caggcggttc aggtaacgct gaggcgtcag
    29041 tcccgtttgc tgcttaagct gccgatgtag cgtacgcagt gaaagagaaa attgatccgc
    29101 cacggcatcc caattcacct catcggcaaa atggtcctcc agccaggcca gaagcaagtt
    29161 gagacgtgat gcgctgtttt ccaggttctc ctgcaaactg cttttacgca gcaagagcag
    29221 taattgcata aacaagatct cgcgactggc ggtcgagggt aaatcatttt ccccttcctg
    29281 ctgttccatc tgtgcaacca gctgtcgcac ctgctgcaat acgctgtggt taacgcgcca
    29341 gtgagacgga tactgcccat ccagttcttg tggcagcaac tgattcagcc cggcgagaaa
    29401 ctgaaatcga tccggcgagc gatacagcac attggtcaga cacagattat cggtatgttc
    29461 atacagatgc cgatcatgat cgcgtacgaa acagaccgtg ccaccggtga tggtataggg
    29521 ctgcccatta aacacatgaa tacccgtgcc atgttcgaca atcacaattt catgaaaatc
    29581 atgatgatgt tcaggaaaat ccgcctgcgg gagccggggt tctatcgcca cggacgcgtt
    29641 accagacgga aaaaaatcca cactatgtaa tacggtcata ctggcctcct gatgtcgtca
    29701 acacggcgaa atagtaatca cgaggtcagg ttcttacctt aaattttcga cggaaaacca
    29761 cgtaaaaaac gtcgattttt caagatacag cgtgaatttt caggaaatgc ggtgagcatc
    29821 acatcaccac aatgaagcaa attgtgaaca tcatcacgtt catctttccc tggttgccaa
    29881 tggcccattt tcctgtcagt aacgagaagg tcacgaattc aggcgctttt tagactggtc
    29941 gtaatgaaat tcagcaggat cacattatga cctttcgcaa ttgtgtcgcc gtcgatctcg
    30001 gcgcatccag tgggcgcgtg atgctggcgc gttacgagcg tgaatgccgc agcctgacgc
    30061 tgcgcgaaat ccatcgtttt aacaatggcc tgcatagcca gaacggttat gtcacctgga
    30121 atgtggatag cctggaaagt gccattcgcc ttggattaaa caaggtgtgc gaggaaggga
    30181 ttcgtatcga tagcattggg attgatacct ggggcgtgga ctttgtgctg ctcgaccaac
    30241 agggtcagcg tgtgggcctg cccgttgctt atcgcgatag ccgctccaat ggcctaatgg
    30301 cgcaggcaca gcaacaactc ggcaaacgcg atatttatca acgtagcggc atccagtttc
    30361 tgcccttcaa tacgctttat caactgcgtg cgctgacgga gcaacaacct gaacttattc
    30421 cacacattgc tcacgctctg ctgatgccgg attacttcag ctatcgcctg accggcaaga
    30481 tgaactggga atataccaat gccaccacca cacaactggt caatatcaat agcgacgact
    30541 gggacgagtc gctactggcg tggagcgggg ccaacaaagc ctggtttggt cgcccgacgc
    30601 atccgggtaa tgtcattggg cactggattt gcccgcaggg taatgagatt ccggtggtcg
    30661 ccgttgccag ccatgatacc gccagcgcgg ttatcgcctc gccgttaaac ggttcacgcg
    30721 ctgcttatct ctcttctggc acctggtcat tgatgggctt cgaaagccag acgccattta
    30781 ccaatgacac ggcgctggca gccaacataa ccaatgaagg cggggcggaa ggtcgctatc
    30841 gggtgctgaa aaatattatg ggcttatggc tgcttcagcg agtgcttcag gagcggcaaa
    30901 tcaacgatct cccggcgctt atcgccgcga cacaggcact tccggcctgc cgcttcatca
    30961 tcaatcccaa tgacgatcgc tttattaatc ctgaggcgat gtgcagcgaa attcaggctg
    31021 cgtgtcggga aacggcgcaa ccgatcccgg aaagtgatgc tgaactggcg cagctacgcg
    31081 gtgaagattt ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc
    31141 agctatgtgc cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcgacgc
    31201 tcggcaatat cggcatccag ttaatgacgc tggatgaact caacaatgtg gatgatttcc
    31261 gtcaggtcgt cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa
    31321 ttgcccacta tgtggcgcag cttcactcta cacgacagac aaaggagctt tgcgcatgac
    31381 cactcaactg gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga
    31441 tgtcgaggag gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg
    31501 cgatgatgtt tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac
    31561 aggcaattat ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc
    31621 tatgcggctg attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga
    31681 tacgccagtc tcgcgcgacc agatcaaacc agagcacttc aaaaactggg gtgaatgggc
    31741 gaaagccaat cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc
    31801 cgatggcttt acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg
    31861 caaagccagc cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat
    31921 gaacatctgg atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca
    31981 gcgtctgctg gcagcactgg atgaggtgat cagcaagaag ctaaaccctg cgcaccatat
    32041 cgacgccgtt gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa
    32101 tgagttttac atggggtatg ccaccagccg ccagactgcg ctgtgcctag acgccgggca
    32161 cttccacccg actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtgccgca
    32221 gttgctgctg cacgtcagcc gtccggttcg ctgggactgc gatcacgtag tgctgctgga
    32281 tgatgaaacc caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca
    32341 tatcggcctt gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac
    32401 acgcaatatg aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa
    32461 gctggaagcg gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt
    32521 gccgtggcag gcggtctggg aaatgtattg ccaacgtcac gatacgccag caggtagcga
    32581 atggctggag agcgtgcggg cttatgaaaa agaaattttg agccagcgtg ggtaaacact
    32641 gccggatgcg gcgcgagcgc cttatccggc ctacgggtcg gcaacagttg taggcctgat
    32701 aagacgcgac agcgtcgcat caggcattga ttgccggatg cggcacgagt gccttatccg
    32761 gcctacaggt cggcaatagt tgtaggcctg ataagacgcg acagcgtcgc atcaggcatt
    32821 gattgccgga tgcggcacga gcgccttatc cggcctacgg gtcgtgcatc tgacaacacc
    32881 gaatttacag gaacacagaa catgcaaaac attacccagt cctggtttgt ccagggaatg
    32941 atcaaagcca ccaccgatgc ctggctgaaa ggctgggacg aacgcaacgg cggcaacctg
    33001 acgctgcgcc tggatgacgc cgatatcgcg ccttataaag acaacgtcca tgcacaaccg
    33061 cgctatatcc cgctcagcca gcccatgcct ttactggcaa atacaccgtt tattgtcacc
    33121 ggctccggca aattcttccg taacgtccag ctcgatcctg cagctaactt aggcgtcgta
    33181 aaagtcgaca gcgatggcgc gggctaccat attctctggg ggttaaccaa cgaagccgtc
    33241 ccgacatccg aactcccggc gcactttctc tcccactgcg agcgcattaa agccaccaac
    33301 ggtaaagatc gggtgatcat gcactgccac gccaccaacc tgatcgccct cacctatgta
    33361 ctggaaaacg acaccgcggt ctttactcgc caactgtggg aaggcagcac cgagtgtctg
    33421 gtggtattcc cggatggcgt tggcattctg ccgtggatgg tgcccggcac ggacgaaatc
    33481 ggccaggcga ccgcacaaga gatgcaaaaa cattcgctgg tgttgtggcc cttccacggc
    33541 gtcttcggca gcgggtcgac gttggatgaa acattcggtt taatcgacac cgcagaaaaa
    33601 tcagcacaag tattagtgaa ggtttattcg atgggcggca tgaaacagac catcagccgt
    33661 gaagagttga tagcgctcgg caagcgcttt ggcgttacac cactagccag tgcgctggcg
    33721 ctgtaaggag caaacatgat ccgcaaagcc tttgtcatgc aggttaaccc cgacgcccac
    33781 gaagagtatc agcgtcggca taaccccatc tggccggaac tggaagcagt gttgaaatct
    33841 cacggtgcgc ataactacgc catttacctc gacaaagcgc gtaatctgct gtttgccacg
    33901 gtagagattg aatctgaaga acggtggaat gcggtagcca gtgctga
//
LOCUS       DAJTOR010000027        32958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTOR010000027 DAJTOR010000000
VERSION     DAJTOR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.027"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..257
                     /locus_tag="OO249_001129"
     CDS             <1..257
                     /locus_tag="OO249_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5635501.1"
                     /translation="LDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNGLSVV
                     MTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            267..1641
                     /gene="gspE"
                     /locus_tag="OO249_001130"
                     /pseudo
     CDS             267..1641
                     /gene="gspE"
                     /locus_tag="OO249_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1631..2833
                     /gene="hofC"
                     /locus_tag="OO249_001131"
     CDS             1631..2833
                     /gene="hofC"
                     /locus_tag="OO249_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5635502.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(2868..3911)
                     /gene="guaC"
                     /locus_tag="OO249_001132"
     CDS             complement(2868..3911)
                     /gene="guaC"
                     /locus_tag="OO249_001132"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5635503.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(4067..4111)
                     /locus_tag="OO249_001133"
     CDS             complement(4067..4111)
                     /locus_tag="OO249_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635504.1"
                     /translation="MIIKTLKMSARKRN"
     gene            4136..4756
                     /gene="coaE"
                     /locus_tag="OO249_001134"
     CDS             4136..4756
                     /gene="coaE"
                     /locus_tag="OO249_001134"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5635505.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4756..5499
                     /gene="zapD"
                     /locus_tag="OO249_001135"
     CDS             4756..5499
                     /gene="zapD"
                     /locus_tag="OO249_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5635506.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            5509..5706
                     /gene="yacG"
                     /locus_tag="OO249_001136"
     CDS             5509..5706
                     /gene="yacG"
                     /locus_tag="OO249_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5635507.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5813..6202)
                     /gene="mutT"
                     /locus_tag="OO249_001137"
     CDS             complement(5813..6202)
                     /gene="mutT"
                     /locus_tag="OO249_001137"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5635508.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(6262..8967)
                     /gene="secA"
                     /locus_tag="OO249_001138"
     CDS             complement(6262..8967)
                     /gene="secA"
                     /locus_tag="OO249_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5635509.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(9029..9541)
                     /gene="secM"
                     /locus_tag="OO249_001139"
     CDS             complement(9029..9541)
                     /gene="secM"
                     /locus_tag="OO249_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5635510.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9772..10689)
                     /gene="lpxC"
                     /locus_tag="OO249_001140"
     CDS             complement(9772..10689)
                     /gene="lpxC"
                     /locus_tag="OO249_001140"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5635511.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(10790..11941)
                     /gene="ftsZ"
                     /locus_tag="OO249_001141"
     CDS             complement(10790..11941)
                     /gene="ftsZ"
                     /locus_tag="OO249_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5635512.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(12002..13264)
                     /gene="ftsA"
                     /locus_tag="OO249_001142"
     CDS             complement(12002..13264)
                     /gene="ftsA"
                     /locus_tag="OO249_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5635513.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(13261..14091)
                     /gene="ftsQ"
                     /locus_tag="OO249_001143"
     CDS             complement(13261..14091)
                     /gene="ftsQ"
                     /locus_tag="OO249_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5635514.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKETAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQLAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(14093..15013)
                     /gene="ddlB"
                     /locus_tag="OO249_001144"
     CDS             complement(14093..15013)
                     /gene="ddlB"
                     /locus_tag="OO249_001144"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5635515.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(15006..16481)
                     /gene="murC"
                     /locus_tag="OO249_001145"
     CDS             complement(15006..16481)
                     /gene="murC"
                     /locus_tag="OO249_001145"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5635516.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16535..17602)
                     /gene="murG"
                     /locus_tag="OO249_001146"
     CDS             complement(16535..17602)
                     /gene="murG"
                     /locus_tag="OO249_001146"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5635517.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17599..18843)
                     /gene="ftsW"
                     /locus_tag="OO249_001147"
     CDS             complement(17599..18843)
                     /gene="ftsW"
                     /locus_tag="OO249_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5635518.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18843..20159)
                     /gene="murD"
                     /locus_tag="OO249_001148"
     CDS             complement(18843..20159)
                     /gene="murD"
                     /locus_tag="OO249_001148"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5635519.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(20162..21244)
                     /gene="mraY"
                     /locus_tag="OO249_001149"
     CDS             complement(20162..21244)
                     /gene="mraY"
                     /locus_tag="OO249_001149"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5635520.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(21238..22596)
                     /gene="murF"
                     /locus_tag="OO249_001150"
     CDS             complement(21238..22596)
                     /gene="murF"
                     /locus_tag="OO249_001150"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5635521.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22593..24080)
                     /gene="murE"
                     /locus_tag="OO249_001151"
     CDS             complement(22593..24080)
                     /gene="murE"
                     /locus_tag="OO249_001151"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5635522.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTVPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(24067..25833)
                     /gene="ftsI"
                     /locus_tag="OO249_001152"
     CDS             complement(24067..25833)
                     /gene="ftsI"
                     /locus_tag="OO249_001152"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5635523.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25849..26214)
                     /gene="ftsL"
                     /locus_tag="OO249_001153"
     CDS             complement(25849..26214)
                     /gene="ftsL"
                     /locus_tag="OO249_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5635524.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(26211..27152)
                     /gene="rsmH"
                     /locus_tag="OO249_001154"
     CDS             complement(26211..27152)
                     /gene="rsmH"
                     /locus_tag="OO249_001154"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5635525.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(27154..27612)
                     /gene="mraZ"
                     /locus_tag="OO249_001155"
     CDS             complement(27154..27612)
                     /gene="mraZ"
                     /locus_tag="OO249_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5635526.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(28214..29218)
                     /gene="cra"
                     /locus_tag="OO249_001156"
     CDS             complement(28214..29218)
                     /gene="cra"
                     /locus_tag="OO249_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5635527.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHLMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(29300..29386)
                     /locus_tag="OO249_001157"
     CDS             complement(29300..29386)
                     /locus_tag="OO249_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5635528.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(29398..29889)
                     /gene="ilvN"
                     /locus_tag="OO249_001158"
     CDS             complement(29398..29889)
                     /gene="ilvN"
                     /locus_tag="OO249_001158"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5635529.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29892..31616)
                     /gene="ilvI"
                     /locus_tag="OO249_001159"
     CDS             complement(29892..31616)
                     /gene="ilvI"
                     /locus_tag="OO249_001159"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5635530.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPISISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            31735..31779
                     /locus_tag="OO249_001160"
     CDS             31735..31779
                     /locus_tag="OO249_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635531.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(31934..32878)
                     /gene="leuO"
                     /locus_tag="OO249_001161"
     CDS             complement(31934..32878)
                     /gene="leuO"
                     /locus_tag="OO249_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5635532.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7912 a         9256 c         8018 g         7772 t
ORIGIN      
        1 gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc acccgctatg
       61 tttcagccat gagtgtggca aaaggcgtgg tgtcgctgac cgggcaagaa agtctcaatg
      121 gactaagcgt cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc ggctgggcgc
      181 gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat gtcttccgct
      241 ttgatgacgc caactaagga gcggcaatga atattccaca gctcaccgcc ctgtgtctgc
      301 gttatcaggg agtcttgctg gatgccagcg aagaggtggt tcatgttgcg gtagtcgatg
      361 caccttcgca tgagttgctg gacgcattgc atttcgctac caccaaacgt attgagatca
      421 cctgctggac gcgccaacaa atggaaggcc acgccagtcg cacacaacag acattgcccg
      481 tagctgttca ggagaagcat cagtcgaaag cagagttgct gactcgaacg ttacaatctg
      541 cgctggaaca acgtgcgtct gatattcata tcgaaccagc ggacaatgcc taccgcatcc
      601 gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat gccggagtcg
      661 cattaaccgc cagattaaaa gtgctgggaa acctggatat tgcggaacat cgcctgccgc
      721 aggacgggca attcactgtc gaactggcag gaaacgccgt ctcatttcgt attgcgacct
      781 taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg aaccaggcac
      841 tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct catgccttgc
      901 aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa acggtcacgc
      961 tttatagtgc cctgcaaaag ctgaataccg ctgacattaa tatttgtagc gtcgaagatc
     1021 cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt gccgggctca
     1081 cctttcaggg cgttttgcgt gcgttattgc gccaggatcc tgacgtcatc atgatccgat
     1141 ggcgaaacgg cagaaattgc cattaaagcc gcgcaaaccg gtcacctggt gttgtctacc
     1201 ctacacacta attccacctg cgaaacgctg gtacgtttac agcaaatggg agtcgcccgc
     1261 tggatgctct catcagcgct tacgctggta atagcccagc gtctggtacg taaattttgc
     1321 ccacattgtc gccagcagca aggggagccc atccatattc cagacaatgt atggccgtcg
     1381 ccgctgcccc actggcaggc acccggttgt gtacattgct accacggttt ttatggtcgt
     1441 accgccttgt ttgaagtcct gcccattaca ccagccatac gccagcttat ttccgctaat
     1501 accgacgttg aatcgctgga aacgcacgca cgacaggcgg gtatgcgtac gctttttgaa
     1561 aacggctgcc tagccgtgga gcaaggctta accacctttg aagagttaat ccgcgtactg
     1621 gggatgccgc atggcgagta agcaactctg gcgctggcat ggcataaccg gcgacggcaa
     1681 tgcgcaagat gggatgctat gggcagagag ccgtgctttg ctgctcatgg cactacagca
     1741 acagatggtt accccactta gcctgaagcg aatcgccatc aattctgcgc agtggcgagg
     1801 agataaaagc gcggaagtca ttcatcaact ggcgacgcta ctcaaagccg ggttaacgct
     1861 ttctgaaggg ctggcactgc tggcggaaca gcatcccagt aagcaatggc aagcgttgct
     1921 gcaatcgctg gcgcacgatc tcgaacaggg cattgctttt tccaatgcct tattaccctg
     1981 gtcagaggta tttccgccac tctatcaggc gatgatccgc acgggtgaac tgaccggtaa
     2041 gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa gcccagcgtc agttgaccga
     2101 caaagtgaaa tcagcgttac gttatcccat catcatttta gcgatggcaa tcatggtggt
     2161 tgtggcaatg ctgcattttg ttctgccgga gtttgccgct atctataaga ccttcaacac
     2221 cccactaccg gcactaacgc aggggatcat gacgctggca gactttagtg gcgaatggag
     2281 ctggctgctg gtgttgttcg gctttctgct ggcgatagcc aataagttgc tgatgcgccg
     2341 accgacctgg cttatagtgc ggcagaaatt gctgttacgc atcccgatta tgggttcact
     2401 gatgcgggga caaaaactca cgcagatctt tacgattctg gcgctgacac aaagtgcagg
     2461 cattactttt ttgcagggcg tagagagcgt cagagaaaca atgcgctgcc cgtactgggt
     2521 gcaacttctg acacaaatcc agcacgatat cagtaacggt catcccatct ggctggcgct
     2581 aaaaaatacc ggggagttta gcccgctctg tttgcaatta gtgagaacag gagaggcatc
     2641 cggctcgctg gacctcatgt tagacaacct cgcccatcat catcgggata acacaatggc
     2701 gctggcggat aacctcgcag ccttactgga accggcgttg ctgatcataa cgggaggaat
     2761 tatcggtacg ctggtggtgg cgatgtatct gccaattttc catttaggcg atgcgatgag
     2821 tgggatggga taacgctggc gtgttgctcc acaccagcgt tggaagatta caggttgttg
     2881 aagatgcggt tttcttgttc ctgcacacgg ataaatgtgg tgcgcttggt cagttctttc
     2941 aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat atcacgcgcg
     3001 gtattttcaa ccgggcctcg cagcggcagc ttaacggttt taccttctgc tgcgcgatat
     3061 tccgcaacgc cgccaacgtg acgtttcatc gcagactcgg agctcatgcc gtagaacagc
     3121 ataaatttct cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg gcccgccagc
     3181 atgccgccaa gcatgacgaa atcggcaccg ccgccaaagg cttttgcgac atcgcccggc
     3241 gtggtgcagc caccgtcgct gacgatcatt ccgcccagac cgtgcgccgc atcggcacat
     3301 tcgattaccg cagaaagctg cggataaccg acgcctgttt tgacgcgagt tgtacaaaca
     3361 gaacctgggc caatgccaac tttaacgata tcagcaccgg agaggataag ctcctcacac
     3421 atttcaccag tcactacgtt gccagcacaa atggttttgg tcggccacgc ttcacgcgct
     3481 ttcgcaacga actgcacgaa gtgttcggaa taaccattcg ccacatcaat acaaacaaag
     3541 tttaatgccg ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc atcagacgta
     3601 ccggtagaaa ccatcacatg tttcagcaca tcagcggaag aattgttgat aaacgcttgc
     3661 cactcttcga cagaatagtg tttatgcaca gcagtcaaaa tatcaaaaga agccagcgca
     3721 gaggccatag aaaatgtacc tacggtgtcc atatttgcgg cgataatcgg cacgccggac
     3781 cagctctgat ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc ggaacggctt
     3841 ttaagagtgg agcgtttagg gcggatgaga acgtctttaa aacctaactt cagatcttct
     3901 tcaatacgca tgtgcggatt cctggggtta atggctataa gggtaagagc gcaactccag
     3961 tgacgctatc atacgcacta atcaatgcgg cgcaagactg cgaaattgtc tttttttacg
     4021 ctacaatccc ataaatttac ctggtaaata gtgagcgaac gtttcatcaa tttcgctttc
     4081 gtgcactcat ttttaacgtt ttgataatca tgatgaattc ccgggaatat aaattatgag
     4141 gtatatagtt gccttaacgg gaggcattgg cagtggcaag agtaccgttg ccaatgcgtt
     4201 tgctgatctc ggaattaacg tcattgatgc cgatattatt gcgcgtcagg tggttgaacc
     4261 aggtgcacct gcgctacatg ccattgctga tcactttggc gctaacatga ttgctgctga
     4321 tggaacattg cagcgccggg ccttgcgcga gcggatcttc gccaacccgg aagagaaaaa
     4381 ctggcttaac gccctgctgc atccgctgat tcagcaagag acgcaacacc agatccagca
     4441 agctacttcc ccctatgtac tgtgggttgt gccattgctg gtagaaaact cactgtataa
     4501 aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa acgcaactta agcgcaccat
     4561 gcagcgcgat gatgtaactc gcgagcatgt cgaacaaatc cttgctgctc aggcaacgcg
     4621 cgaagcccgc cttgccgtgg cagatgacgt cattgataat aacggcgcac cggatgctat
     4681 cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc agtttgtctc
     4741 acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac atccactaaa tgaaaaaatg
     4801 cgtacatggc tgcgcattga gtttttgatt cagcaactca ccgttaattt acccatcgtt
     4861 gaccacgctg gcgcgctgca tttcttccgt aatgtcagtg aattactgga tgttttcgag
     4921 cgcggcgaag tccgcactga gctgttgaaa gaacttgacc ggcagcaacg taaactccag
     4981 acctggattg gcgtgcctgg cgtggaccag agccgtattg aagcattaat tcagcagtta
     5041 aaagcggcgg ggagcgtatt aatttccgcg ccgcgtatcg ggcaatttct gcgtgaagat
     5101 cgtttgattg ctctggtgcg tcagcgactg agcatcccag gcggctgttg cagctttgat
     5161 ttacctacat tgcacatttg gctgcatcta ccccaggcgc agcgcgacag ccaggtagaa
     5221 acctggattg ccagcctgaa cccgctcacc caggcactta ccatggtgct ggatttaatt
     5281 cgccagtcgg cccccttccg taaacaaacc agcctgaatg gtttttatca ggataacggt
     5341 ggcgatgccg acttgctgcg cctgaatctg tcgctcgatt cacagcttta tccgcaaatt
     5401 tccggacata agagccgttt tgccattcgt tttatgccgc tggacagtga aaacggacag
     5461 gtaccggaac gtctagattt cgaactggcc tgttgctaag gagtgaaaat gtcagaaact
     5521 attacggtga attgcccaac ctgcgggaaa acggtggtat ggggtgaaat cagcccgttt
     5581 cggccatttt gctccaaacg ttgtcagctg atcgacctcg gagaatgggc tgctgaagaa
     5641 aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg actggagcga agagccaaag
     5701 cagtgacatt ggtctgatgc cgcacatagg cctgataaga cgcggacagc gtcgcatcag
     5761 gcatcttgtg caaatgtcgg atgcggcgtg aacgccttat ccgacctaca gactacagac
     5821 gcttaagctt cgcaattacc ggttcattgg ctggcggaaa atcatcggca ttaagaccga
     5881 ccagcgacat ccactcaccg ggttgccctt ctttacccca cggcacccct tcccaacttt
     5941 cgaccagcca gaaccacaga gttatatgcc tgtccgggaa ttcatatgcc agtttttcaa
     6001 atagcgaaaa atgttgtggg gtaatcccga cctcttcctg aagttcacgc accaccgcct
     6061 gttccggcgt ttcgcccatt tcaattttac cgccgggaaa ctccagttta ttcgccatgt
     6121 gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt ctcgttgcga ataataccta
     6181 ccgcaatttg cagctttttc attgttttaa acctataaaa aaggcgcaga atcctgcgcc
     6241 ttttgcttca acagttagct tttattgcag gcggccatga cactgcttgt attttttacc
     6301 agaaccgcac gggcaaggat cgttacgtcc tactttgcgc tcaccggttt gcgccgccag
     6361 tgcagctgcg gctgcagagt cgtcatcctg atggctaagc tgctgcattt gcgctaaacg
     6421 ctcggcttcc atacgacgct gttgttccag ctcctcaacc tcttcaggca tacgtacctg
     6481 aactttgctc agcgtactga taacttcata tttcaacgac tccagcatcg ctgcaaacat
     6541 ggagaacgat tcacgtttgt attcctgctt cggatctttc tgtgcatagc cacgcaggtg
     6601 gataccctga cgcagatagt ccatcgctgc caggtgctct ttccacaggg agtcaagcgt
     6661 ttgcagcatg acgcctttct cgaagtgacg catcatctca gcaccaacca cttcttcttt
     6721 acgctgatac acttcgatgg actgcgccag aatgcgctca cgcagcgtct cttcatgcag
     6781 ttctggttct ttatccagcc actcggcaat tggtaaatcg aggtcgaaat cgttcttcag
     6841 acgttcctgc agccccggaa tatcccacat ttcttccagc gactgtggcg gaatgtaggc
     6901 atcaatggtg gctttgaaca catcttcacg aatgctgttg atggtttcgc tcacatcgct
     6961 gacatccagc agttcgttac gctgggagta aatggcgcga cgctgatcgt tagccacgtc
     7021 atcatattcc agcagttgct tacgaatgtc gaagttacga ctttcaactt tacgctgggc
     7081 gttggcaatc gctttagtca cccacgggtg ttcaatggct tcgcctggct tcatacccag
     7141 tttacgcatc atgccggata ctcggtcgga agcaaaaata cgcatcagcg catcttccat
     7201 cgacaggtag aaacgggaag aaccagcatc cccctgacga ccagaacgac cgcgcaactg
     7261 gttatcgata cgacgggatt cgtgacgctc ggtaccgatg atatgcaggc cacctgcttc
     7321 cagtaccgca tcgtgacgta cctgccagtc ggctttaatt ttttcaattt gctctgcggt
     7381 cggattttcc agcgcggcaa cttctgcctg ccagctacca ccgagcacaa tatctgtacc
     7441 acgacccgcc atattggtcg cgatagtcac cgcagccgga taacctgctt gagcaacaat
     7501 cgccgcttcg ttggcgtgga atttggcgtt caggacgttg tgcttaatac cggctttggt
     7561 cagttcgttt gacaccagct ccgatttttc gatggagata gtacccacca gcaccggctg
     7621 gcctttcgca gtacgttctt tgatatcttc aatgatcgcc tgaatttttt ccgcttcagt
     7681 catgtagacc aggtccggca gatctttacg aatcattgga cggttggtcg gaacaacgac
     7741 ggtatccagc ttgtagatgg agctaaattc gaaagcttcg gtatcagcag taccggtcat
     7801 ccccgccagt ttttcataca gacggaagta gttctggaag gtgatcgaag ccagcgtttg
     7861 gttttcgttc tggatctgca caccttcttt cgcttccaca gcctggtgca gaccatcgga
     7921 ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca acgatgataa cttcaccatc
     7981 tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga gcgcgcagcg ccgccgttac
     8041 gtggtgcatc agcatgatgt tggccggaga gtacagagac tccccttcat ccatgatgcc
     8101 ctctttcacc agcagttctt caatcagcac cagaccacgt tcggtcaggt tcacctggcg
     8161 agatttttcg tccaccgaga agtggccttc gccctggaag gtttcggagt cttctttttc
     8221 ctgacggatc aggtgcggaa taattttatt cacgcgttta tacatttccg agctgtcttc
     8281 tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg atcaggatgg agtccacttc
     8341 gtccaccagc gcatagtgca gtttacgctg tacacgttct tcagggctga acgccatatt
     8401 gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg taagtgatgt cagctgcgta
     8461 agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg ataccgacag tcaggccaag
     8521 gaattcaaac agcggacggt tgttttcggc gtcacgttgc gccaggtagt cgttgacggt
     8581 aactacgtgc acgcctttac cggttagtgc gttcaggtaa gcaggcagcg ttgcggtcag
     8641 ggttttacct tcaccggtac gcatttcggc gatgcagcgt tcgttaagaa ccataccgcc
     8701 gagtaactga acgtcgaagt gacgcatacc aaagacgcgc ttacttgcct cacgtaccac
     8761 ggcgaaagct tccgggatca gattttccag cacttcgcct ttttccagac gtgcacgaaa
     8821 ctctgcggtt ttccctttca gttcttcgtc ggagagtttt tccatctccg gttccatggc
     8881 attgatgatg ttgaccactt tgcgcatccg gcgcagggtg cgatcgttac gactaccgaa
     8941 aactttagtt aacaatttga ttagcataat aaaatctcaa acgccccgcg ttgcggagtt
     9001 aataaaatga agtaaaggtt tattgttgtt aggtgaggcg ttgagggcca gcacggatgc
     9061 cttgcgcctg gcttatccag acgggcgtgc tgaattttgc ttgtggggta aaatgcgcat
     9121 aatcaatgcg ataacccttt tcagacggcg tgccttcctg ggtcagcagc gcgctgagcg
     9181 tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga ttcttcagca acgggcagtg
     9241 tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt gcgaatggca tattgatgcc
     9301 agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt cgcttccagc aaggccaatt
     9361 gaccaaagtt aactttggct gaaggctcgt ggttgcgggt tgtcgctttt gcgggcacgt
     9421 ttggttcggc ggcgttgctg agcgcaggca aacctaaact cgccgcgacc atccctaata
     9481 agagatgcgg ccagaagtag cgtttaccaa actgtcgcca gcgcgtcagt attccactca
     9541 cgttattgcc atcccgatcg aattcaaacg tatttagcgc acaaatctta tcattaaagc
     9601 ctgaagtcca cagcatgaat gaagataagt tgtgcgtaaa agtgcttaaa aaaacagcgt
     9661 tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa acgactggtt cacctggccg
     9721 gagagagtgc cagatttgcc agtcgaattt tatacgacag tataaatgtc gttatgccag
     9781 tacagctgaa ggcgctttga aggccaacgg cagttctgcg tcgtcctgga aggtcacata
     9841 ttcccaagct tcctgtttcg ccaggacagc ctgcagcagt ttgttattca gtgcatgacc
     9901 ggatttataa gcgataaatg caccaataat attgtgacca cacatgaaca agtcaccgat
     9961 cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa cgcaggccgt cttcgttcag
    10021 tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg ccgcccaggc acaaaccacg
    10081 ggactgcaga tattcgatat cacgcatgaa accgaacgta cgcgcacggc tgatctggcg
    10141 cataaacgca tcagcggaga agttcatcgc atagcgctgg ttgctggaat caatagccgg
    10201 atggttaaaa tcgatggtga aatccagcga aaaaccattg tacggcttaa attcagccca
    10261 cttatcgcca tcttcgacac gaacagtctc tttgatgcga acaaattttt tggcgcagtt
    10321 caactcgtcg ataccggcgt caagcagcag gtatacaaac ggagcggcgc tgccgtccat
    10381 gatcgggatt tccggcgcgt taacttcgat aacaatgtta tcgatgccca ggcccgcgag
    10441 agcagcattg aggtgctcta cggttgaaat ccgtacatca tgctcgttga ccagacacgt
    10501 acagagcatg gtatcacgca cagatttggc atcggccggg aaatctaccg gtggattcaa
    10561 gtcggtgcga cgatagatga ccccggtgtt ggccggcgca gggcgtaacg tcagagtgac
    10621 tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg atacgtttaa gtgtcctttg
    10681 tttgatcatc gtattatctc gccaaattac ctatccaacc gaagtgtact atacattcgg
    10741 cgggccagtt tagcacaaag agcctcgaaa ccaaaattcc agtcaattct taatcagctt
    10801 gcttacgcag gaatgctggg atatccagat aatccggctc tttcgcagtt tgcggcgcat
    10861 tgtcattcac gactttagca accggcttct gctcctgggt cagcggagcc atcccatgct
    10921 gctggtagcg atccatcact ggctgctgaa cctgcttatt ggtcaccaga gtgatttcag
    10981 gacgtttgtc catgccgata cctgtcgcaa caacggttac gcgcagctcg tcattcatat
    11041 ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc ggaagcaaat gcacggatgg
    11101 tgttacctac cgtttcgaac tcatccagac gcaggtcgaa gcccgccgtg atgttaacca
    11161 gcacgccgcg cgcgccagac aggtcgatat cttccagcag cggagaagag atagccattt
    11221 cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc agaacccatc attgcgtagc
    11281 ccatctcaga cattacggtg cgtacgtctg caaagtccac gttcatcaaa cccggacgag
    11341 taatcagttc agcgatacct tgcacagcgc ctttcagtac atcgttcgct gcgccaaacg
    11401 catccagcag ggagataccg cggcccagaa ctttcagcag tttgtcgttc gggatagtga
    11461 tcagagagtc cacatgcttg gacagttcag tgatcccctg ctccgcgaat gccatacgct
    11521 tcttgccttc aaagttgaaa ggcttagtga cgacagcaac ggtcaggata cccaaatctt
    11581 ttgccacttc agcgacgact ggtgctgcac ctgtaccggt accaccaccc atacccgcag
    11641 caataaagac catgtctgca ccttccagcg ccgcacgcaa tgcatcgcga tcctcatcag
    11701 ccgcattgcg gccaacttct ggattagcgc cagcgcccag tcctttggtg ataccgctac
    11761 cgatttgaat cgtctgtcca accgctgttt tacgcagcgc ttgtgcatcg gtatttaccg
    11821 cgaagaattc aacaccttca atgcgctcgc gcaccatgtg ttcaacagca ttaccgccgc
    11881 cgccgccgac gccgatgact ttaatcaccg cgtcattggt aagttccatt ggttcaaaca
    11941 tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa tcatcgtcgg cctcataaaa
    12001 attaaaactc ttttcgcagc caactattga gtcgcttgat ccacgagcca actgatgctg
    12061 taacacgttt ttctacttca gcttcaccgt taagatgtga ctctttccca tagtgaagca
    12121 atcccaccgc cgtcgaataa tacggctcct gagcataatc cgttaaaccg gtaatgttca
    12181 gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg agcacaggct gcaagacctt
    12241 cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc caggtgatgt ttaacccctt
    12301 gttggcgaag cttttcctgc aactgcaata tctcttcgtt gaccaggttg agcagctcgg
    12361 tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg cagactccgt ggcggacgac
    12421 cgcctacgct cggcacttcc acgctctcat cttttccaac gatggaaccc agcgcacaac
    12481 cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt gccaaaggcg taagcgatat
    12541 cactggtcac gacattgcca gcataaggaa ttaccttagt gtggcgcaat gccccaccgg
    12601 tataaacggc gatatccatt gtaccacctc cgatatcgac gacgcagaca cccagttcac
    12661 gttcatcttc cgtcaatacc gaataacttg atgccagtcc ggcaaatatc agttggtcaa
    12721 ctttcagccc acaacgttca accgctttga cgatgttttt cgccatatcg ttgtgacatg
    12781 tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag tcctaccgga ttcttgatcc
    12841 cttcctgata gtcaatcgca tactcttgcg ggatcacatg cagcacacga tgttcatcgc
    12901 gcacacgcac cgatttcgcg gtatggacga cgttttccac atcttcttgc gtcacttctt
    12961 cttcagaaat aggcaccata ccaatttcat tctggcagct gatgtgctta ccagaaagcg
    13021 ccagatatac cgaagagatc tgacaatctg ccatcaattc tgcctggtca atggcgcgtt
    13081 gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc tttatccata ccacgtgacg
    13141 ggcagctgcc cacgccaatg atattgacca taccgtcggg cagaacttcc cctactaaag
    13201 cggcaacctt cgcggtacca atctccagtc ctactaccag ttttctgtcc gtcgccttga
    13261 tcattgttgt tctgcctgtg cctgattttg ttgctgagta gattcctctg gcggcaaggg
    13321 cgcccagcct actgccgctc cagagtcata acgcaaatca acgtagctaa tccgtttgcc
    13381 atcggtttgc gccagctgct gtaaaaccgg ataaagttct acaaagcgag ccaaacgttt
    13441 catcgtatcg ccccggccaa gattgagctt aatatcgtta ttcagcgtca actgccagga
    13501 acgccgcgcg gtcatcgccg tttccttcag agtaaatctg tcctttgcca gcatctgccc
    13561 catttcgcga tagccctgca acacttcatt ggcgctgcct tccgggccat acagcattgg
    13621 aagcacctgc ttgctggtgc gatctggcgg cacgctgaag gtgtttcctt ccgcgtctac
    13681 catatgttga tcattccacc gcgcaatcgg cacatattca accagatgaa tcttcaattc
    13741 atcaggccac tgctttctga cgctcacctg cttaatccac ggcaggcgtt gttctatttg
    13801 cgtctggatg atgttgacat cctgggtcat aaaggtaccc ggctcaccca atgccaggat
    13861 cgactgccgg atatcgtcat tacgcgtgta atggcgttca ccggtcaaca ccagctttga
    13921 gagcggcagg cgttgcgcat cttccatcca gcccaacacg acccagccgc tcaccaacac
    13981 tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga cgcgttccat tattgcggcg
    14041 agaagaaacc tcttcttcgc tgtttcgcgt gttcagagca gcctgcgaca tattagtccg
    14101 ccagttccag aattcgtact accaactgcg agaagctcat acctgcctga cgtgccgcca
    14161 tcggcaccag gctgtggctg gtcatacccg gtgaggtatt ggcttccagc agataaaact
    14221 gtccatcgct gtccagcata acgtcaatac gtccccatcc tttgcaacct aacgtcgtcc
    14281 atgctttcag cactaatgcc tgcaaattgg cctcttgtga cgcatccaga cctgcggggc
    14341 agaaatactg tgtctcatca gagagatact tcgcctcata atcatagaag gttccggacg
    14401 gttgaatacg tattgacggt aaaatttctt caccgagtat cgcaaccgtg aactccggcc
    14461 cacttagcca tttttcaatc aatacttctt catcgtgctg aaatgccaat cttaatgcat
    14521 cttgtagagc attttctgct actacttttg acattcccac actggaacct tcgcggctcg
    14581 gcttaacgat aaccggcaaa cccagagcag aaatttctgc taactgctta tcgctcaggc
    14641 ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc gaccggtaaa ccggcacctt
    14701 gccacagcag tttgctgcgt agtttatcca ttgaaagcgc agatgccatc actccgctgc
    14761 cggtataagg caagcccatc agctcgagca tgccctgcag cgtaccatct tcaccgccgc
    14821 gaccgtgtag cgcgataaac actttctgaa aacccatcga cttcagttgc gtcacgtcga
    14881 cttctttcgg gtcgacagga tacgcatcaa taccaccttc acgcagcccg gctaacaccg
    14941 ctgcgccaga attcagagaa acttcccgct cagcggaagt cccgcccaac aggaccgcga
    15001 ttttatcagt catgttgttc ttcctccgga gtttgcggct tcagtttgat ttcagctaaa
    15061 gaacgggcaa tttttccgat attaccagcc ccctgaacga gaatcaggtc gttaccggtt
    15121 aataccggtg ccagcatctc ggctacctgc gcagggtccg gcaccagaat gggatcaatt
    15181 ttcccacgtc cacgaattgt gcgacacagc gaacggctgt ccgctcccgg aattggcgct
    15241 tcgccagccg gatacacttc cagcatcaac agggtatcaa cctgcgtcag cacattggcg
    15301 aaatcatcat acaagtcgcg cgtacgggta aaacggtgcg gctgaaacag cattaccagg
    15361 tttttatccg gccagcctgc gcgcgccgct ttaatggtgg cgtcaacttc cgtcgggtgg
    15421 tggccgtagt catcgaccag cattgccgta ccgcttttac cattcactgg ctccagcggg
    15481 aattcaccga ggaaatcaaa acggcgacca gtccccagga agctctccag cgcacgcaaa
    15541 atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa ccgcagctgc ggcgttcagc
    15601 gcgttatgac ggcctggcgc attcagggtg acgcgcatcg gctctttgtc ctggcgcagc
    15661 agcgtaaagt gcccctgcgg gccaatctgc tgataatctt ctacacgcac gtcggcatct
    15721 tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg gtaacaattc gcggatcacc
    15781 ggatcatcaa cacacatcac cgcacgaccg taaaacggca ggttgtgcag aaaattaata
    15841 aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat ccatgtggtc ggcttcgata
    15901 ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg atgcatcact ctcatctgct
    15961 tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa cccccgccgc ttttaccagc
    16021 ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt agatgctgga aaccatcgcg
    16081 gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc catgacgaaa acgcattaac
    16141 tcagccagca tttcggcacg acggatcacc ggaatacgcg cttcatgagc tgcgacaatt
    16201 tccgggttat cggcagaaat ggcgctggaa acaacgacca cgctggcatc acgtacgttt
    16261 tccgggcgat ggttgaaata aatcgtcgct cccagattca ttaactgctg cgtgaccgga
    16321 tttggcgcta aatcggaacc actgatctga taaccttcat tggccagaac ttcggcaata
    16381 ccgcccatac cggcaccacc gatgccgaca aaatgtatgt gccgaacgcg acgcatttcg
    16441 ggcacgatgg aacgcagttt tgccaattgt tgtgtattca ttctttacgc cattaacttc
    16501 ttaaattcat acgatgcaaa aggcatcgct acaattacgc ccgggcagcc cggctcactt
    16561 catttgccac tcgctcggtg gcatccggaa tggatgcagc gcgggcgcgt tctgccatgg
    16621 ttaataaggt ttctcgcgac cacccggcca gggtgttggc gacagcatcc acgctaagct
    16681 gtggctgctc gataattttg gctgcgcccg ctttttccag cggtagcgca ttccagtatt
    16741 gctggcggtc tttatgttga aacggcacaa acaacgccgg tagtcctgcc gcggcgattt
    16801 cactcaccgt taaagcaccg gagcggcaaa cgacgacatc cgcccacgca tacgccgccg
    16861 ccatatcatc aataaattcc gtcactttat gctgcggttg ccccgcttcg gcatacgcct
    16921 gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca gatagtgact gaatcaccca
    16981 gtttcgcagc aacctgcggc attgtctggt taagaatgcg tgcgccctgg gaaccaccca
    17041 ctaccagcac acgaaccgga ccttcacgtc cagccaaacg ttgctgcggc aacggcagcg
    17101 ccagcacatc ggtacgcacc gggttaccca ccacttccgc attagggaaa gcgcctggaa
    17161 acgcctgcat cactttggtg gcaatcttcg ccagccattt attggttaag cccgcaatac
    17221 cgttttgttc atgaagtaca accggaatgc ctaacgacca cgcggccaga ccacctggac
    17281 ctgatacata gccgcccata ccgagtacca cgtcaggttt gtacgctttc ataatcgccc
    17341 gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc tatcagtgct tttatacctt
    17401 ttccacgcag accagagata cgaatgaaat caatttcgat gccatgtttt ggcactaagt
    17461 ccgcttccat acggtcggca gtccccagcc agcgaacttg ccaaccctga gccattagat
    17521 ggtgcgcaac cgccagtccc gggaatacat gtccaccggt tccgcctgcc atcaccatta
    17581 atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac gcctgcgctt tctccagacg
    17641 cgtttcataa tcaatacgca acagcatcat gatggctgtc gacataatca gtaagctcga
    17701 accaccgtaa ctgatcagcg gcaatgtcag acctttggtc ggtaacatcc ccgccgccgc
    17761 gcctacgtta accagcgcct ggaagctaaa ccagatgcca atagaacagg cgagaaaacc
    17821 ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc gacatcgcgc gaaaagcgac
    17881 gaagaatacc attaaaagtg ccagcaccac accgacatac cccagttctt cgccgataat
    17941 ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc agtttttgta ccgagttacc
    18001 taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc agcgattgcg ttaactgata
    18061 gccgctgcca aagggatctt cccacgggtt ccagaatgcg gtaacacggc ggatacggta
    18121 cggttcggcg agtatcagca acacaaccgc tgaaatgccc ataccgataa tggcaatgaa
    18181 ctgccacaat ttcgctcccg ccaggaacaa catcgccagc gtagtcacaa acaccaccac
    18241 caccgtacca aggtctggct gtgccagcag taacactgcc aacaccagaa tcacgcccat
    18301 cggtttcagg aagccgcgca ggttattacg tacttcgtcg cctttacgca ccagatagtt
    18361 ggcgatataa caaaacagcg acagttttgt cagctccgca ggctggatac gcagcaaacc
    18421 gagatcgatc caacgcgatg cccctttaac cgagctcccc actaccagca cgatcatcag
    18481 caggatgata gatccgagca gcatcgtggc actgtagcgt tgccagaact ccatcggcag
    18541 acgcagcgta atgatcgcca gaatgaatgc caggatcaga tagacgccat cacgcttcgc
    18601 gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc attgacgccg aggtcaccat
    18661 gataaagcca atcgccgcaa ggccgaaggt cagccacagt aaggtgcgat cgtacatgat
    18721 caggctgtcg gtatcttttt cccgcgagcc catcacccag ccttttagcg ccgtggagat
    18781 ccagaccagg atactgaatc ctggcaggcg cggcattttc aggcgaggga gagataaacg
    18841 catcaaccta actccttcgc cagacgggca aactcattgc ctcgttgttc aaagttcttg
    18901 aactgatcaa ggctggcaca ggccggggag agcagaacca tatcgcccgg ctgaacacgc
    18961 ggagccagca agcgcatcgc ctgttccata gtttcggttt gttctgccac ttccgggcgt
    19021 agcgccgcca gctgcgcacc gtcacgacca aaacagtaca aacgcacgtt gtcgccattc
    19081 agatagcgcg ccagtggact gaagtccgcc gatttaccat cgccgcccag caacaaatgc
    19141 agcgtgccgt ctacctgcaa gccattcaac gccgcttccg tactgccgac gttagtggct
    19201 ttcgaatcgt taatccagcg tacgccattg tgctccagca ccacttcaaa gcgatgcggc
    19261 agaccggtga aggtggttaa cgctttcagg ctgctggcgc gcggcaaacc agctgcgtct
    19321 gccagtgcca gtgctgccag cgcattggtg tagttatgct gcccggaaag tttcatctct
    19381 ttcacgttca gcactttctc gcctttcact cgcagccagg tttctccctg ctgatgattc
    19441 aggtgatagt cacccatgtt gacgccaaag ctgacgcagc gttcatcagc accgcgaatc
    19501 ggcattgtta aggcatcatc agcattgacg acacaaactt tcgcattttc gtaaatacgc
    19561 aattttgccg cacggtattg ctgcaaacca aacggatagc ggtccatatg atcttcagtc
    19621 acgttcagaa tggtcgccgc caccgcctgt aagctggaag tggtttccag ctggaagctc
    19681 gacagttcca gcacgtacag ttcgcactca gcatcaagca gcatcaacgc tggcaggcca
    19741 atattgccac ccacaccaac gttaaccccc gccgctttcg ccatttcacc cactagcgtg
    19801 gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa tcggtgcttg tgcttcgcgg
    19861 cagaacagct cgatatcgcc aacgatttcg attccggcat cagcggcagc gcttaaggat
    19921 ggatgcgcca gtgcaatacc gggactggcg acaatcagat ctgccgccat cagccattca
    19981 tcattcagac tgcccgtgtg gcgttctacg gcttcgggta atttatccag gccaggcggt
    20041 gtcatacgcg tatccataac gcgcggcgtc acaccgcgag cgaggaaaaa gtccacgcag
    20101 gaaagcccgg tgaggcccag gccgataatg acgacatttt taccctgata atcagccatg
    20161 attaacgtac cttcagcgtt gccagaccaa tcagaaccag catcagcgaa ataatccaga
    20221 aacgcacaat gacgcgcggt tccggccagc ctttcagttc atagtggtga tgaatcggtg
    20281 ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc gacctgcagg atgacagaaa
    20341 gcgtttctac cacgaacacg ccccccataa tcaccagcag gaattcctga cgtagcagta
    20401 cggcgataat gcctaacgca ccacctaacg ccagcgaacc tacatcgccc ataaagacct
    20461 gcgccggata ggtgttaaac cacaggaagc ccagtcctgc cccgactatc gcggtacaga
    20521 caataaccag ttccccggcg tgtcgcagat acggtatgtg taaatagctg gcaaagttca
    20581 tgttgccggt cgcccacgcc accagcgcaa aaccaccggc gacaaatacg gtcggcataa
    20641 ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt gccagtaccc acaatgacga
    20701 agtaagccag cagaatgtag aacagcccca actgcggcat cacatcttta aagaatggga
    20761 ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta cagggcgaag gcgacaccca
    20821 gcgcaatgac cgacatccag aaatacttcc aacgagcgat caaccctttg gtgtctttac
    20881 gcaccacttt gcgataatca tcaacaaagc caataacacc gtaacctacc agcaccacca
    20941 acacgcacca gacgtacgga ttggacgggt aagcccacag cagtacggag atcacaatcg
    21001 ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg cttgctgaag tgtgattcag
    21061 gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg caaatgagca atcatacgcg
    21121 ggcccatcca caatgagatg aacagcgcgg tcagcaggct gacgatggcg cgaaacgtca
    21181 gataggaaaa gacgttaaag ccggaataat atttgaccaa atgttcggcc agccaaacta
    21241 acatgtccca ttctcctgta aagcgcgtac tacctcttcc atggcggcac tacgtgaacc
    21301 cttaactaaa atcgtaatta cctgttgctc agcaatcagt gatttaagac gcgtaattaa
    21361 cgcagtttta tcagcaaaat gttcgccaac gccgctggcg gtgctgatag catggctttg
    21421 tttacccacg cttaacacgc ggtcaatacc agcagctttt gccgcctcgc ccacctgtac
    21481 atggcaggct tcgctttcag cgcccagttc cgccatatcg cccaccacca gcacgcggta
    21541 gcccggcatt tcagccagta cctgaacagc ggcggtcatt gaaccgacat tggcgttgta
    21601 ggagtcgtcg agcagcaact ggttttctgc cagttggatg gggaacagac ggcctggaac
    21661 agctttcaga tttgccagcc ccgctttgat agcatcaagc gttgcgccca cggacatgga
    21721 gagcgcagcg gctgccagcg cattcgcaat attgtgacgc cccggcaacg gcagcagaac
    21781 atcgacgcta ccggttgggg tttgtagggt aaattccgta ccgtgcgagg tcacatggat
    21841 attggtggcg gtgaaatcgc tgttggcggc attgggtgag aaacgccaca ctttgcgtga
    21901 gccaattacg ctctgccagt tcagccagtc gttgttgtcg gcgttcataa tggcgatacc
    21961 gttttccggc aggccgctaa agatttcacc tttcgctttc gcgacacccg caagcgagcc
    22021 aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc gcagcttccg ggcgagtcag
    22081 actcacagtc caggctattt cgccctgatg gttcgcgcca agttcaataa ctgcgtaatc
    22141 gtattccggc gttaagcgca acaacgtcat cggtacaccg atgtcgttgt tgagattgcc
    22201 tgccgtataa agcgtgttgc cgcactggct taaaatcgcc gccgtcatct ctttaacgga
    22261 ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc ggaacttgct ggcgaaccca
    22321 tgcagccagt tcaccaaacg ccagacgcgt atccttgacg attaactgcg gcaggtcgat
    22381 atccagcgga cggctgacca gtagtgcgcc cgcgccgcca gctttcgcct ggtcggcaaa
    22441 atcgtgggca tcaaaacgtt cgcctttcag ggcaacaaac aggcagcccg gcgtcagttt
    22501 tcgggtatca gtggttacag catcaagggt gatatctgca ccttgcagtt caccgttgag
    22561 aatgtcggta agttggctaa gggttacgct aatcatgcaa tcacccccag cagacgcgcc
    22621 accgtgacgc gatcggagta gtccagacgc tgattgccaa caatctggta atcttcatgg
    22681 cctttgcccg cgaccagcac cacatcgttc tctttagcct gcataacggc gcaagtcacc
    22741 gcttcagcac ggccttccat cactttggca tgtccggcat ctaacattcc cgccagaata
    22801 tcgttgatga tggcacgcgg ttcttcggta cgcgggttat cgtccgtcac caccgccacg
    22861 tcagcaaact cttcggcaat tgcgcccatc agtggacgct tacctttatc gcgatcgcca
    22921 ccacagccaa agacacacca cagcttgccc gcacagtgca gacgcgccgc ctgtaaggct
    22981 ttttccagtg catccggcgt atgcgcgtaa tccaccacca ccgtcggttt gcctggcaca
    23041 gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg cggcggtttt caacagatca
    23101 gccaatggat agccgagtgc caacagtgtc gccagcgcga gcagcaggtt gctgacgtta
    23161 aaagcgccta tcagatggct ttcaatttcg ccatcgcccc aacttgagct aaagcgaatc
    23221 gtcacaccgc tgtcgtgata gttcacgtcg atcactttta accagcgtcc gtgacagttc
    23281 ggattaatat gatcttccat tgataccgca accgcgtccg gcagctttgc cagccaacgg
    23341 cggcccactt catcgtcggc gttaataatc gcctgaccgc aatgatgttc agaataaagc
    23401 agccatttcg cagcttcgta gtgttccata tcaccatgat aatcaaggtg atcgcggctt
    23461 aagttggtaa agaccgacgc cgcaaatttc aatgccgcca cacggtgctg taccagcccg
    23521 tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct ccaccagccc cgccagctca
    23581 tgctgaacat cgactgccga accggtggta ttttctgtcg ggatcacttt ccccagcaga
    23641 ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa gcagttggct ccactgcgcc
    23701 agcagctggg tagtcgtggt tttgccgttg gtgcccgtta cgcctacgag acgtaaattg
    23761 tcagagggtt catggtaaaa gcggcccgcc agtgcagata aacgctcgtt gagctggctg
    23821 agatagatga ccggtacgcc gtgcatttca cggatttcac cgtcggtcgc ctcatctttc
    23881 gcctctgcaa taatggcagc cacaccttgc gctatcgcct gcgggatata tcgacgcccg
    23941 tccgcctgat gacctactac agctacaaag agatcgcccg ccgcagccac acggctgtcg
    24001 agtgtcatct ctcgcagtgc tcgcgaaggt gcgtctggca cccacggagc aagaaggtcg
    24061 cgcaaattac gatctgccac ctgtcccctc gccttgatta atcacaaatt catttttatc
    24121 gcccgttgtc agcgcatccg gctcgatgtt catggtacgc aatacgccgc ccatgatggc
    24181 accaaagacc ggcgcggaaa cggcgccgcc gtagtattta cccgcctgcg gatcgttgat
    24241 aacaacaacc agcgcgaagc gcggctgact cgcaggcgca acgcctgcgg tataagcaat
    24301 atatttattg atgtagcgac cgtccggccc gacctttttc gcggtaccgg ttttaatggc
    24361 gatacgatag cctttaatcg ccgccttcac gccgccgccg cccggtagcg ccacgctttc
    24421 catcatatgc accacggtgc ggacaatgga ttccgggaag acacgttcac cgggaaccgg
    24481 ggggtcaact ttggtaatcg acagtgggcg ataaatgccg tagctgccga tagttgcgta
    24541 gactcgcgct aactgtaacg gtgttaccat tagcccgtag ccgaaagaga aggtggccct
    24601 ctctatgtca gaccaccgtt gtttttgagg atataagcca ctgcgttctc cgaccaaccc
    24661 caaattggtc gcttttccca gtccaaaacg tgagtaagta tctactaacg ctgaggacgg
    24721 catcgctaac gccagcttgg aaacaccgac gttactcgac ttctgtaata ccccggtcag
    24781 ggttaattcg ctgtagcgtg ccacgtcttt gatttcgtgg ccgttaattc gataaggaat
    24841 ggtattgagt accgagtttt cccgcaccac gccacgttgc aacgcggtca tcaccaccat
    24901 cggtttaacc gttgagcccg gttcaaacac gtcggtgatg gtacggttac gcatcgcctc
    24961 tttcggcgtg ccgctcagat tgttagggtt gtatgacggg ctgttagcca tcgccagcac
    25021 ttcacctgtg ttgacatcca ccagcacggc gctaccagat tcagccttgt taaaggccac
    25081 cgcgttgttc agttcgcgat aaaccagcgc ctgcaggcgt tcatcaatac tcagcgccag
    25141 gttgtgcgct gcctggctgt cagtagaaga aatatcttca attacgcgac catagcggtc
    25201 tttacgcaca atgcgctcac ccggctgccc ggtaagccat ttatcgaaac tcttctcaac
    25261 gccctcaatc ccttgactat cgacgttagt aaagccaatg aggtgagcag tcacttcgcc
    25321 ggatggataa taacggcgag attcttcacg cagatgaata cccggcagtt tcagtttttt
    25381 gatgtagtcc gccatgtcag ggtttacctg acgcgccaga taaataaagc gccctttcgg
    25441 gttggcgtta atgcgggcag aaagctgatc cagcggaata ttgagcgcgt tagccagcgc
    25501 cttccagcgg tcaccgacgc tgataccgcc agcgtcatgc acttctttcg ggtcagccca
    25561 aatcgctttt accggcacgc tcaccgctaa cgggcgacca gaacggtcag taatcatgcc
    25621 gcgggaggtg gaaacttgct gaacgcgaag agaacgcatg tcgccctctt tcaccagcat
    25681 atccggggag ataacttgta accacgctac gcgtccgagc agaaaagcca gcgccaggag
    25741 aatacagccg cataacaacg caaaacgcca actaataaag ttggcatgtt cttcctgacg
    25801 ttttggtttc tgcgttttcg ccgctgcttt catgcgtcgc gtttatcctt atttttgcac
    25861 tacgatattt tcttgtgacg gatcaacatg ctgcatttgc agcttttccg tggcgatcct
    25921 ttccacccgg ctatggtcgc cgagcgcatt ctcttcaagg atcaggttgc gccattcaat
    25981 gtctaaagca tctcgctcca gcaccagttg ttcgcgctga gcggtcagta aacgggtatg
    26041 gtgcgccgtg gttaccacgg tcaccgccgt caaaataatg caaatgaaca ggcagagtgg
    26101 cagcttccca aatcgcaaaa gatcgtcacc gataacacca ggcaatgcat ggcgctcgtg
    26161 gcttcccatc gatcctttaa ctttgcttag agcttctgtc actctgctga tcatgcgttc
    26221 gtcctctctg cgatacgcag aactgaacta cgggcacgag gattctcagc cacctcttct
    26281 tcgcccggca ttaacttgcc taatgctcgc agctgacggc cacccagttt tttgagctgc
    26341 tcttcagtca tcggtaaccc tgccggaact tgcggaccgc ggctgttttc acgcataaaa
    26401 cgtttcacaa tacggtcttc cagcgagtgg aagctgatga tcgaaagccg cccacccggg
    26461 gccagcacgt tgagcgagct ttttagcgcc tgctctatct cctccagttc actgtttacc
    26521 caaatgcgca ccgcctggaa ggtacgggtc gcgggatgtt taaacttatc tttcaccggc
    26581 gttgcagcag ccacgacttc cgccagttct ttggtgcggg tcatcggctg ttcgcggtta
    26641 cgctcgacaa tggcgcgggc aatgcgtttg gcaaaacgct cttcaccata ggttttcaat
    26701 acccaggcga tatcggcttc ttctgcggtt tgtagccatt cagcggctga ctgcccacgg
    26761 gttgggtcca tacgcatgtc cagcggacca tcgcgcataa aggaaaagcc acgttcagca
    26821 tcatcaagtt gcggtgaaga gacgccaaga tcgaggagaa tgccgtcgat cttgccgata
    26881 agatcgcgct cggcaacgta ttcgcccagc gcggagaaag gtccgtggat gatggagaag
    26941 cgcggatcat caatagtctt cgcaacggcg atagcctgcg ggtcgcgatc gatcgccagc
    27001 aaacgcccct cttcgccaag ctgcgagagg atcagacgtg agtgaccacc gcgaccaaaa
    27061 gtcccatcaa tgtagatgcc atcaggacgg atattgaggc cattaacggc ttcatccagc
    27121 agcaccgtag tatgtttata gttttccatc attttataga gacaagtcct gcagtcgctc
    27181 cgataagtct ccagtagcca actgctctgc gtcgatatct tccttgacct gttgatgcca
    27241 ggttgtttca tcccacagct caaacttgtt gaactgtcca accagcatca cttcttttgt
    27301 cagcccggca tgttgccgca gtactggcgc gattaacaat cgacctgcgc catccatctg
    27361 acattcgctg gcatgaccta acagtaggcg ctgcacacgg cgctcaactg ggttcatgct
    27421 cgacagacgc gataattttt gctcgataat ttcccattca ggcagggggt aaagcagcag
    27481 gcacgggtga tgaatgtcaa tggtgcaaac catttgaccg gcagcgttct caagcagctg
    27541 ttcccgataa cgggtaggca ctgataagcg ccctttgctg tcgagattga ctaacgttgc
    27601 tccccggaac atgccagcct caccccttat tcccactttg ccccacaatt tcccactgaa
    27661 aggagtttac ggagcggagg aaaagcttgt caagccagtc acaaggcata ccgaggtaaa
    27721 aaacccgatg tttacggcta atatcagcgt tgtttatttt acgtccaacg cgcgaagcaa
    27781 attaacgtta tgaatagctg taagaaaaaa tccctcatcg tcatcactgt tttaaaatga
    27841 ctcactattt tccacagcaa atataaagtg tcagtttgcg acgcgagcgg cattttagga
    27901 catatcttcc ccggttaaca gtccttgtta cgtctgtgtg gcgcttgatg ccagcgggtt
    27961 tgcgcaaaag caaagaaaag tgtttgttaa atcggcaatt catcgccgca tgtaacaaaa
    28021 taatgcaaaa acgcgctgtc gatatcactt aattattgtg aatttcagct ggtgagttaa
    28081 ttaacgggcg ggtttaaaac catttatcaa taatagtcag gaatagtctt atttacttta
    28141 agcatattga tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat
    28201 ttttgttcgc ggcttagcta cggctgagca caccgcggcg atagagatta cgtttaatgc
    28261 gcgttaaacc aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca
    28321 cacgctctgc gacatcgcgg tgacgttgag ccactgccag caccgggcac tgtaagaagt
    28381 cgagcagttc gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttaccatcgc
    28441 gacgtagcgt gacatccatc accccttgca acaacgcaaa cgacgttgtg aacagcgcct
    28501 gcggcatcag atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat
    28561 agctgttggc atacaggaaa tgcacttcgc gcggatcatc tttccaggca gtacggaaac
    28621 cttgttcacg caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct
    28681 cggcgggaaa cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa
    28741 ccacgctggt gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggg
    28801 cgttagccca gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg
    28861 catcaacctg acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt
    28921 ctgagcaggc aatcagcagt tgataacccc gttgccgcgc ctggcgctca agatagttag
    28981 cgatgcgggt atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc
    29041 gtccagcacg aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag
    29101 ccatgacttt ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa
    29161 catagcttgc agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa
    29221 ttgccccttg cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt
    29281 aacatctaag cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt
    29341 tcgcaaaaaa agcccagcgt gacatgttgg gctgcaaatt gcacactgag atcattctca
    29401 acgcattatt ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat
    29461 tttcgccacc tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa
    29521 ttgaacggta taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt
    29581 acgtttcact tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg
    29641 ctcaacatgc gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa
    29701 ttgcttttcg atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga
    29761 taatgtcgga tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga
    29821 aaaaaggcca atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat
    29881 ccggcgcata atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg
    29941 cgaatctgca tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg
    30001 cgattattgc gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga
    30061 gagatctgga tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat
    30121 tgcatataag attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc
    30181 cccagatagc ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta
    30241 gacagttcct gaatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct
    30301 tctggcaacg ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgagg
    30361 ccaccggaat tgatccagcg acgcggtttg tcgaatggat aataaagtgc agcaaacatc
    30421 tggtgctgcc cgacatcgga cgtcacgtaa gcgtctccct tcgtcaaccg ccaaagagtc
    30481 tcgatcaccg cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgacga
    30541 gcgcgccact gttcaatttg ctgccaccag tcgcggatct catccagtgg ttgatgggcg
    30601 gattcttgcg acaagagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc
    30661 gggatatccg cagtcacggt tttagaaatg gaaataggat caatatcgat atgcagaaca
    30721 gtggcatttg ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg
    30781 acggcgaaaa tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt
    30841 cccagcatgc ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag
    30901 gaaacaacgg gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc
    30961 atgattgccc caccgcctac gtagacaacc ggtttttttg ccactaccag cgtttgcaga
    31021 gcacgcttaa tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc
    31081 gactccggcc agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca
    31141 acgactactg gtccaggacg accacttgcc gccagccaga aagccttttt cagcacctgc
    31201 ggaatgtctt ccgtttgctt aaccagaaaa ctgtgtttaa ccactggtcg cgaaatcccc
    31261 accatgtcgc actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa
    31321 aggacaacta atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc
    31381 gcccctggac ccgacgttac cagcacgacg ccgacttccc cggtcgcgcg cgccagacca
    31441 tcggccatat gcactgccgc ctgctcatga cgaactaaaa catgatcgat accacccacg
    31501 gtatgcaatg catcgtaaat atcaaggact gcgcctccag gataaccgaa tacttgttta
    31561 acgccctgat cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc
    31621 tgcctcactg tttgatggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag
    31681 caattggcag ccagagacat aaacgcaggg taataagaat aaacaggagg aaagatgaaa
    31741 agaaacgata aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca
    31801 caacttacag caatttctct atttttttaa ttaatcctaa aatagagagc ccacacattc
    31861 agaataaaat tctacattca ataaaaaatg cagaataaac cagacattca tgtctgacct
    31921 attctgcatt cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc
    31981 ctttatcgcg cccggcagct tcatgccatg agagataaca ggttctgctg ttttgtttta
    32041 acggcagcgg taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg
    32101 gcgcaatagc gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc
    32161 cctgatacgc gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg
    32221 cgaaacgatc gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg
    32281 ggcctttaat tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg
    32341 gcacgctggt aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct
    32401 gataacgcag ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat
    32461 ttggcgcaat ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc
    32521 tgcaaacaca aagatgaaat acacgttcac tactcgcggg ttcaaaacct gaaccaggca
    32581 attcattttg tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag
    32641 cagtcggttg aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca
    32701 ggcgtgcaac agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg
    32761 taatgttttg ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc
    32821 gtagctgtgg cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac
    32881 ttaactccac tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg
    32941 aagaatatga tttatcat
//
LOCUS       DAJTOR010000028        32422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTOR010000028 DAJTOR010000000
VERSION     DAJTOR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.028"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            198..992
                     /gene="panB"
                     /locus_tag="OO249_001162"
     CDS             198..992
                     /gene="panB"
                     /locus_tag="OO249_001162"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5635533.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1004..1855
                     /gene="panC"
                     /locus_tag="OO249_001163"
     CDS             1004..1855
                     /gene="panC"
                     /locus_tag="OO249_001163"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5635534.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2147..3043)
                     /gene="rpnC"
                     /locus_tag="OO249_001164"
     CDS             complement(2147..3043)
                     /gene="rpnC"
                     /locus_tag="OO249_001164"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5635535.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQGKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3317..3697
                     /gene="panD"
                     /locus_tag="OO249_001165"
     CDS             3317..3697
                     /gene="panD"
                     /locus_tag="OO249_001165"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5635536.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3701..4901)
                     /locus_tag="OO249_001166"
                     /pseudo
     CDS             complement(3701..4901)
                     /locus_tag="OO249_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727225.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(4996..5436)
                     /locus_tag="OO249_001167"
     CDS             complement(4996..5436)
                     /locus_tag="OO249_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5635537.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5541..6311)
                     /locus_tag="OO249_001168"
     CDS             complement(5541..6311)
                     /locus_tag="OO249_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5635538.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(6308..7234)
                     /locus_tag="OO249_001169"
     CDS             complement(6308..7234)
                     /locus_tag="OO249_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5635539.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKLETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7343..8005
                     /gene="can"
                     /locus_tag="OO249_001170"
     CDS             7343..8005
                     /gene="can"
                     /locus_tag="OO249_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5635540.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8046..8582)
                     /gene="hpt"
                     /locus_tag="OO249_001171"
     CDS             complement(8046..8582)
                     /gene="hpt"
                     /locus_tag="OO249_001171"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5635541.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8788..11178
                     /gene="gcd"
                     /locus_tag="OO249_001172"
     CDS             8788..11178
                     /gene="gcd"
                     /locus_tag="OO249_001172"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5635542.1"
                     /translation="MAINNTGSRRLLVTLTVLFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPTEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPKLKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11225..12775)
                     /gene="cueO"
                     /locus_tag="OO249_001173"
     CDS             complement(11225..12775)
                     /gene="cueO"
                     /locus_tag="OO249_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5635543.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12941..13288
                     /locus_tag="OO249_001174"
     CDS             12941..13288
                     /locus_tag="OO249_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5635544.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13394..14260
                     /gene="speE"
                     /locus_tag="OO249_001175"
     CDS             13394..14260
                     /gene="speE"
                     /locus_tag="OO249_001175"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5635545.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14276..15070
                     /gene="speD"
                     /locus_tag="OO249_001176"
     CDS             14276..15070
                     /gene="speD"
                     /locus_tag="OO249_001176"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5635546.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15107..15469)
                     /locus_tag="OO249_001177"
     CDS             complement(15107..15469)
                     /locus_tag="OO249_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5635547.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15644..18241)
                     /gene="acnB"
                     /locus_tag="OO249_001178"
     CDS             complement(15644..18241)
                     /gene="acnB"
                     /locus_tag="OO249_001178"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5635548.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGLLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFMNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18596..20449
                     /locus_tag="OO249_001179"
     CDS             18596..20449
                     /locus_tag="OO249_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR5635549.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAAVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSNEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYRMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRERVQESRPQRLNDSNRTARLNNDQRSAVRERLSERGARRLER"
     gene            complement(20520..21944)
                     /gene="lpdA"
                     /locus_tag="OO249_001180"
     CDS             complement(20520..21944)
                     /gene="lpdA"
                     /locus_tag="OO249_001180"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5635550.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22269..24161)
                     /gene="aceF"
                     /locus_tag="OO249_001181"
     CDS             complement(22269..24161)
                     /gene="aceF"
                     /locus_tag="OO249_001181"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5635551.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24176..26839)
                     /gene="aceE"
                     /locus_tag="OO249_001182"
     CDS             complement(24176..26839)
                     /gene="aceE"
                     /locus_tag="OO249_001182"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5635552.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27000..27764)
                     /gene="pdhR"
                     /locus_tag="OO249_001183"
     CDS             complement(27000..27764)
                     /gene="pdhR"
                     /locus_tag="OO249_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5635553.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28305..29678
                     /gene="aroP"
                     /locus_tag="OO249_001184"
     CDS             28305..29678
                     /gene="aroP"
                     /locus_tag="OO249_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5635554.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29721..30575)
                     /gene="ampE"
                     /locus_tag="OO249_001185"
     CDS             complement(29721..30575)
                     /gene="ampE"
                     /locus_tag="OO249_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5635555.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30572..31123)
                     /gene="ampD"
                     /locus_tag="OO249_001186"
     CDS             complement(30572..31123)
                     /gene="ampD"
                     /locus_tag="OO249_001186"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5635556.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31211..32104
                     /gene="nadC"
                     /locus_tag="OO249_001187"
     CDS             31211..32104
                     /gene="nadC"
                     /locus_tag="OO249_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5635557.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32307..>32422
                     /locus_tag="OO249_001188"
     CDS             32307..>32422
                     /locus_tag="OO249_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5635558.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAAL"
BASE COUNT         7815 a         8813 c         7991 g         7803 t
ORIGIN      
        1 ggccacgcta caacaggatg gaactatacc aatagaagct ggtgagttta aagccaccag
       61 tactttccag gtaacctacc cttaataagt ccaccgcacc gccatcatct ggcggtgcgg
      121 taattgataa atcattgccc ccaccaatgt gactgataaa atgccaggca acacgacatc
      181 atttatcagg acacgttatg aaaccgacca ccattgcttc acttcagaaa tgtaagcagg
      241 ataaaaagcg tttcgcgacc atcaccgctt acgactacag cttcgcgaaa ctatttgctg
      301 atgaaggact taacgtcatg ctggtaggcg actcgctggg catgacggtt cagggtcacg
      361 actccaccct gccggtgacc gttgccgata tcgcctacca cactgcctcc gtacgtcgtg
      421 gtgcgccaaa ctgcctgctg ttggctgacc tgccgtttat ggcgtatgcc acgccggaac
      481 aagcctttga aaacgccgca acggttatgc gtgccggtgc caatatggtc aaaattgagg
      541 gcggtgagtg gctggtcgaa accgtaaaaa tgctgaccga acgtgccgtt cctgtatgtg
      601 gtcacttagg tttaacccca cagtcagtga atattttcgg tggctacaaa gttcaggggc
      661 gcggcgatga agcgggcgat caactgctca gcgatgcatt agccttagaa gccgctgggg
      721 cacagctgct ggtgctggaa tgcgtgccgg ttgaactggc aaaacgtatt accgaagcac
      781 tggcgatccc ggttattggc attggcgcag gcaacgtcac tgacgggcag atcctcgtga
      841 tgcacgacgc cttcggcatt accggcggtc acattcctaa attcgctaaa aatttcctcg
      901 ccgaaacggg cgacatccgc gcggctgtgc ggcagtatat ggctgaagtg gagtccggcg
      961 tttatccggg cgaagaacac agtttccatt aaggagtcac gttgtgttaa ttatcgaaac
     1021 cctgccgctg ctgcgtcagc aaattcgccg cctgcgtatg gaaggcaagc gcgtggcgct
     1081 ggtgcctact atgggtaatc tgcacgatgg ccatatgaag ctggtcgacg aagccaaagc
     1141 ccgcgccgat gtggtcgtcg tcagtatttt cgttaacccg atgcagttcg accgcccgga
     1201 agatctggca cgttatccac ggaccttgca ggaggactgc gagaagctaa acaaacgtaa
     1261 agtggattta gttttcgccc cttcggtaaa agagatctac ccgaacggta ctgaaaccca
     1321 tacttacgtt gacgttcccg ggctttcgac catgctggaa ggcgcgagcc gtccggggca
     1381 ttttcgcggc gtttcgacta tcgtcagcaa gctgtttaac ctggtccagc cggacatcgc
     1441 ctgcttcggt gaaaaggact ttcagcaact ggcgctaatc cgcaaaatgg ttgccgatat
     1501 gggcttcgat attgaaattg tcggcgtccc gattatgcgc gccaaagatg gccttgcgct
     1561 cagttcccgt aatggttatc tgacggcgga acaacgcaaa attgcgcccg gtctgtacaa
     1621 agttttaagt tcaattgccg acaaattgca ggctggcgag cgggatcttg atgaaattat
     1681 tgccattgca gggcaagaac tgaatgaaaa aggcttccgc gccgatgata ttcagattcg
     1741 cgatgccgac acgttgctgg aagtctctga aaccagcaaa cgagctgtaa ttctggtagc
     1801 cgcctggctt ggcgatgccc gcctgatcga caacaaaatc gtcgaactgg cgtaatgctt
     1861 ttactctaaa taattcaagt tgcaggaagg cggcaagcga gtgacaaatt cgtcaggaac
     1921 gaatttgccc agccgcaggc tggccttcgg tgagagacat ggatgtctct cattaatccc
     1981 caggagctta cataagtaag tgactggggt tagcgaacgc agacacagca catgcaattt
     2041 aaagtatgac gagtataact ggcgctacgg ctgatggcgc caattattaa tttgccccac
     2101 gtcatggcga tggcgtctgt aagcaattat tcataacgca gacaacttag ttaatcagcc
     2161 tatcaatctc agcaagagat aaatttgtca tctctgcaac gtcttcccga gacatccctt
     2221 tactcagaaa acgctgggcg aattcttgcc tttcctcctg tcttccctgc tgaatcccct
     2281 tctcaatccc tttctcttca aaccactgcg ccagcgtcat catagactcc cctcccgttt
     2341 ccctgtctct cagcacacca taaaacaaat ccgcttgttc agtatgaccg cgttgcagca
     2401 tatagttttg catggcaact aactgacttc cgctagtgta cccttcgtca atcagcgtga
     2461 ccagttgctc cagcaataac attaagtcgc gctggcgaat atgtttttgc agtagttcga
     2521 gaatcgcaat ccgccgatgt tgcatgattt cgtcatccgg tgtgatggtg atatccacca
     2581 gcgggaaagg actgttatag acgcgtcgtg ccagctccgg cgagtaaaat atatcaaacc
     2641 agcacattga gagcggataa ggtgtggcct cgccctgata aaacagtatc ggcaccacca
     2701 gcggcagctt atcgtgatcc gcctccagat gccggtgcat ggcagcaata gaataacgca
     2761 tcatgcgaaa ggccattttc ttatccggct tgccttggtg ttcaatcaca acatgcagat
     2821 aaccgggatt gccctgcatt tgcacggaat agagcacgtc cgtgctgtgt cctttcaggc
     2881 tctcttcaat gaaactcccc gactctaaat gaagcgtgtt gaggtcacaa agttctcgta
     2941 attccactgg caaatgtatc tccagaaagt cgcgagccgt ctccgcgtgc attaaaaatt
     3001 gtttaaacac tgcatcatgc ggtgtggtac tcggtgcatc catcttccct gccttccgtg
     3061 ttgtcttgcg tgaaggcagt atccagtcaa ggcgtgccat cgtcagccac ctctttagtg
     3121 tttaccgagc agcgttcaga gaaatttcaa ttggttacag ccgttacatg gcacgcttcg
     3181 gcacggtacg caaactgctg atgtaaatgc cgtcagatga gtagacacta aacaaaaatc
     3241 gggcaatact gcgtgagaat ttcctcaaag caggcatcgc ctgcttcgtt aacgacaggg
     3301 tagaaaggta gaagttatga ttcgcacgat gctgcagggc aaactccacc gcgtgaaagt
     3361 gactcatgcg gacctgcact atgaaggttc ttgcgccatt gaccaggatt ttcttgacgc
     3421 agccggtatt ctcgaaaacg aagccattga tatctggaat gtcaccaacg gcaagcgttt
     3481 ctccacttat gccatcgcgg cagaacgcgg ttcgagaatt atttctgtta acggtgcggc
     3541 ggcccactgc gccagtgtcg gcgatattgt catcatcgcc agcttcgtta ccatgccaga
     3601 tgaagaagct cgcacctggc gacccaacgt cgcctatttt gaaggcgaca atgaaatgaa
     3661 acgtaccgcg aaagcgattc cggtacaggt tgcttgattg ttatccctgc ggctggttac
     3721 tcaccagccg cgacatcgtc tccagcgaat ccgttcttaa gatataaagt cgttttagta
     3781 acaacggatt atcccccggt tttactttgc ctttcatggt tgtcaccgcc aggtgaaatc
     3841 cggcatcgtt tgctgccttc acggctttgt cattaaatcc gccaaacgga tacgaaagat
     3901 accagacatg cggattaaat tgcgccagag cgcggcgtga acgtgcaaaa tcaaacagaa
     3961 tattgtgttc actacggctc ggtaatatgg ggcggcgata accatccacc cgatgcaaaa
     4021 aatgggtatg tgactggaaa tcaaaaacat cgcgaatttc gttaagttca gaaacgctca
     4081 taaattgcag cgattttggg tcccacttct gcgggtgacg tttgatgcgc gaggtaacaa
     4141 taaacgccgt cgccttcatg ccatattgtt tcaacacggg atacgcatag cggctcaccg
     4201 atttgaggcc atcatcaaag gtaatcacca ccgctcgcgc agggagattg atcttattct
     4261 tcacgtaacc ttccagctgc accatgctca gtgtcgcgta tcccctgtcg cgcagccagg
     4321 ccatctggtt attgaaagcg cgtactgagg tggtcgtcga agtatggcga aaacgggtgt
     4381 tttcttcatc gcgcagaata tggtgatagg ttagcaccgg caggccatta tcgggttggg
     4441 catccagcgc gctgatatag gccagtcgat cgccaatacg gatctgatac caggtttgat
     4501 ttaacctgtc tttcagttta tgcaaaatcg ggtagcgcaa attgtccgcc agtaccccat
     4561 aatggcgcac tgcccgcact cggcgcgtta tagaccggcg tatctttcca ggtaattaag
     4621 ttctgattac tcagcggctt gttgaggtca cccaaaccgt cttcaacttt ttgtcgcccc
     4681 tgaactggct cgagatgccc tttatcgata aaccctttac caaagccaaa attaaatgcg
     4741 taataacttg cggcagtggg ctccacggca atgatttgcc cggcccgaat attccccacg
     4801 gtcaccatct tgtcgccaat ttgcgcccag atcgcagcat tttcgatggt ttgcatataa
     4861 cgggcaggta acgcggcact aacgcttgcg gtaaacagca tcagcaggat gaataacagc
     4921 ttgtttatac atgaaaaaat aaccgagggt gaaacaacgc ttgtattgta gcaaaagcgc
     4981 catcagaatc gcgggttaat actcatacaa attatgtttg aggacaaaag gcggattttt
     5041 ttgttgttgg tgccaaagac tactcacctc cggcgcaccc agttcgacaa tacgctcgcg
     5101 aaactctgaa ctggtcatgg tttcacggca agccatcatc tgttcaatta acggtaacgt
     5161 gacaccagaa atcacttcac aacgggagtg tttatggctt aataatgaag ccacgcggta
     5221 cggtggcgct ccggctatat cggttaagaa gatgacaccc tcaccagagt ccgcttcatg
     5281 cagagcatcg cacatcatgc ggctgagcat attagagctt aatccgcgcc agaaattcac
     5341 ggcccggcac tgaagaagtg ccccatgttt tttctccagc gcatccagta tctcttgcgc
     5401 ccggtcatcg tgacaggtaa ttacccaacc taacatcgcc ttcctcctta gccgaaaatc
     5461 agtctatcct gaccattgtt gataaagggt gatagccgtc aaaactcctc tcccccgaat
     5521 ccagaggaga ggaaatagcc ttagctacgc aaaccacgtc cacgttggat cagcgaccaa
     5581 cagatcaaat aaaacaccac aataaagacc accagtacgc caaaggtagt ggccagcggc
     5641 acatcattta taccgaggaa gccgtagcgg aaaccactga tcatataaac gattgggttc
     5701 aggtgcgaca gcccttgcca gaacggcggc aacaaagtca gggagtaaaa caccccgccc
     5761 aaatacgtga gtggcgttaa cacaaaggtt ggcaccaggc tgatgtcatc gaacgttttg
     5821 gcaaacacgc cgttcagcaa acccgcaagg gagaatagca ccgccgtgag caccagggtt
     5881 aaggcaacga atacccacga atgcacctga aacggcacaa aaaacagtga aattgccgtc
     5941 accagaatgc caacaaacag accacgcgcc acgccaccgc cgacatatcc ggcaataatg
     6001 acgtgagtcg gaaccggcgc taccagcagc tcttcaatat tacgctggaa cttggcacca
     6061 aaaaatgatg acgcgacgtt agcgtaggca ttggtgatca ccgacatcat gatcagcccc
     6121 ggtacgatga actgcatata gctgaagcca tgcatatcgc caatacgcga acctatcagg
     6181 ttaccgaaga taataaagta aagggtcatg gtgatgactg gcggcaccag cgtctgcacc
     6241 cagatacgca taaagcgatg gatctctttc gcccagatgc tttttagcgc cacccagtaa
     6301 agatgcatca tgcgcgatct ccttgttttt cattaaccag tgaaacaaac agctcttcca
     6361 gacggttagc tttgttacgc atacttaata cctgaatgcc ctgctcactt aactgcgtaa
     6421 atacgctgtt gatcccctgc tcacgcagca cttcaacttc cagcgtcgcg gtatcgacca
     6481 gtcgatactg atagccatcg agcttcggta acgggctttt cggtgcgaga tcgagaataa
     6541 aggtttccaa tttcagcttc gccagcagcg ccttcatcga ggtattttcc accagctcac
     6601 cgtgttgaat aatgccgata ttgcggcaca gcatttctgc ttcttccagg tagtgtgtag
     6661 tgagaatgat ggtggtgcct ttgtcgttta aatccttcaa aaagccccac attgagcggc
     6721 gaagttcaat atccacgcct gcggtcggtt cgtcgagaat cagtagttta ggttcatgca
     6781 ttaacgcacg ggcaatcatt aaacggcgct tcatcccgcc agataacata cgcgcacgtt
     6841 cgttgcgttt tccccataaa tcgagttgtt tgagatattt ttcgctgcgg atgtacgctt
     6901 ctttgcgctc cacgccgtag taccctgcct gattcaccac aatttgctgc acggtttcaa
     6961 acgggttgaa gttaaattcc tgcggcacca gtcccaactg acgtttagcg ttcacgacat
     7021 ccttctcgag atcgtaacca aatacgctga cccgcccgga ggttttattt accagagagc
     7081 tgataatacc gatagtggtc gatttcccgg ccccgttcgg cccgagaagc gcataaaaat
     7141 cacccgcttc gacctgcaaa tctattccac gaagcgcctg aacgccgcct ggataggttt
     7201 ttttaagctg ttgaagttcc agtgcaatgg tcataaattt ttacttacct tacgttctta
     7261 cactttatct gtggtttaaa tcgtcccgga gttgccctat attagccaaa cgtaattatt
     7321 tggttacagg tcgttaacct ccatgaaaga catagataca ctcatcagca acaatgcact
     7381 atggtcaaaa atgctggtgg aagaggatcc cgggtttttt gagaaactgg cacaagcgca
     7441 aaaaccgcac tttctatgga ttggatgttc cgacagtcgc gttcctgcag aacgtttaac
     7501 cggtcttgag ccgggcgaac tctttgttca ccgtaatgtt gctaacctgg tcattcacac
     7561 cgacctgaac tgcctttccg tggttcagta tgcagtggat gtactagaag ttgaacacat
     7621 tattatctgt ggccactacg gttgcggcgg cgtacaagcc gcagttgaaa acccggaact
     7681 ggggcttatc aacaactggc tgctgcatat ccgcgatatc tggttcaaac atagctcatt
     7741 gctcggcgaa atgccgcaag agcgccgtct ggataccttg tgtgaactga acgtcatgga
     7801 acaggtgtat aacctaggcc actccaccat tatgcaatca gcgtggaaac gcgggcagaa
     7861 agttaccatt cacggctggg cctatggcat tcacgacggc ttgctgcgtg atctggatgt
     7921 tactgccacc aaccgcgaaa cccttgagca acgttaccgt cacgggattt ccaacctcaa
     7981 gctgaaacac gccaaccaca aataaaaatg ccatgccgga tgcaacacat ccggcaactt
     8041 cacacttact cgtccagcag aatcactttg ccgatatacg gcagatgacg gtaacgctgt
     8101 gcgtaatcaa tgccgtaacc caccacaaac tcatccggga tcgagaaacc gataaattct
     8161 accgggacgt tcacttcacg acgggacggt ttatccagca gcgtacaaat cgccagcgac
     8221 ttcggttcgc gcaggcttaa gatctcacgc actttcgaca gtgtattccc cgagtcgatg
     8281 atatcttcaa caatcagcac gtccttgcca cggatatctt catccagatc tttgaggatt
     8341 ttcacatcac gggtggtgga catgccgcta ccgtagctgg aggcggtcat aaagtcgact
     8401 tcatgagata cctgaacttc acggcacagg tccgccataa acataaatga gccacgcagc
     8461 agacccacca gcaccatatc gctgccgctg tctttgtaac gctcagtaat ctgacgaccc
     8521 agttcggcga tacgcgcttt aatctccgct tcggggatca ttacttctac agtatgtttc
     8581 atatctctaa ccatatgatt taaaaacaaa tcacttcgca cgggcaggtt ttggcaccgc
     8641 aacaaagcgc aagacacgca gtataccagt aaaactattc attcgcggaa tctgttaata
     8701 aagctcgaat tgtgatgacg atcacacatg ttaaaaccta taattaattg ctactaaaat
     8761 attaatgaat tgaaatggtg tctctttatg gcaattaaca atacaggctc gcgacgatta
     8821 ctcgtcacgc taacagtcct ttttgcagcg ctttgcgggc tgtatctact cattggcgga
     8881 ggctggctgg tcgcgattgg cggctcctgg tactacccta tcgctggcct tgtgatgctc
     8941 ggcgtcgcct ggatgctgtg gcgcagtaaa cgtgccgcgc tttggctata cgccgccctg
     9001 ctgctcggca ccatgatttg gggcgtctgg gaagttggtt tcgacttctg ggcgctgact
     9061 ccgcgcagcg atattctggt cttcttcggc atctggctga tcctgccgtt tgtctggcgt
     9121 cgcctggtca ttcctgccag cggcgcagtt gccgcactgg tggtggcact gctgattagc
     9181 ggtagtatcc tgacctgggc cggatttaac gatccgcagg aaatcaacgg caccttaagc
     9241 gccgatgcca cacctacgga agctatctca cccgttgccg atcaggactg gcctgcctat
     9301 ggtcgcaatc aggaaggtca acgcttttca cctctgaaac aaattaacgc cgataacgtc
     9361 cataatctga aagaagcctg ggtgttccgt actggcgatg tgaagcagcc gaacgatccg
     9421 ggcgaaatca ccaatgaagt gacgccgatt aaagtgggcg acacccttta cctgtgtacc
     9481 gctcaccagc gcctgtttgc gctcgatgcc gccagcggca aagagaaatg gcattacgat
     9541 cctaagctga aaactaacga gtctttccag cacgtaacct gccgtggcgt ctcttatcat
     9601 gaagccaaag cagaaacggc ttcgccggaa gtgatggcgg attgcccgcg tcgtatcatt
     9661 cttccggtca atgatggtcg actgattgcg attaacgctg aaaacggcaa actgtgcgaa
     9721 accttcgcca ataaaggcgt gctcaatctg caaagcaata tgccagacac caaaccgggt
     9781 ctgtatgaac cgacttcgcc accgattatc accgataaaa ccatcgtgat ggcaggttca
     9841 gttaccgata acttctcaac ccgcgaaacg tctggcgtga tccgtggttt tgatgtcaac
     9901 accggggagc tgctgtgggc ttttgatccc ggcgcgaaag atccgaacgc aatcccgtct
     9961 gacgaacaca cctttacctt taactcgcca aactcctggg caccagcggc ctatgacgcg
    10021 aagctggatc tggtctatct gccgatgggc gtgaccacgc cggatatctg gggcggtaac
    10081 cgcacaccgg aacaggaacg ttatgccagc tcgattctgg cgctgaatgc cactaccggg
    10141 aaactggcgt ggagctacca gaccgttcac cacgacctgt gggacatgga tcttccggca
    10201 cagccgacgc tggcggacat caccgttaat ggtcagaaag tgccagttat ttacgctccg
    10261 gcgaaaaccg gcaacatttt tgtgctcgat cgtcgcaatg gcgaactggt ggttccggcc
    10321 ccggaaaaac cggtgccgca aggtgctgcg aaaggcgatt acgttactcc gacccagccg
    10381 ttctctgaac tgagcttccg tccgaagaaa gatttgagcg gtgcggatat gtggggagcc
    10441 accatgtttg accaactggt gtgccgcgtg atgttccacc agatgcgcta tgaaggcatt
    10501 ttcacgccgc catctgaaca gggcacgctg gtcttcccgg gtaacctggg aatgtttgaa
    10561 tggggcggga tttccgttga tccaaatcgt gaagtggcaa ttgccaaccc aatggcactg
    10621 ccgtttgttt cgaaactgat cccacgcggt ccgggtaacc cgatggagca gccgaaagat
    10681 gccaaaggca cgggtacgga atccggcatt cagccacagt acggtgtacc gtatggtgtc
    10741 acgctcaacc cgttcctctc accgtttggt ctgccgtgta aacagccagc atggggttat
    10801 atctcggcgc tggatctgaa aaccaatgaa gtggtgtgga agaaacgtat tggtacgccg
    10861 caggacagta tgccgttccc gatgcctgta ccggtgccgt tcaatatggg tatgccgatg
    10921 ctgggcgggc caatctccac ggcgggtaac gtgctgttta tcgccgctac ggcagataac
    10981 tacctgcgcg cttacaacat gagcaacggt gaaaaactgt ggcagggtcg tttaccagcg
    11041 ggtggtcagg cgacgccaat gacctatgaa gtgaatggta agcagtatgt ggtgatctcc
    11101 gcaggcggtc acggttcatt tggtacgaag atgggcgact atattgtggc ttatgccctg
    11161 ccggatgatg tgaaataacc ataatgcccg gagagatccg ggcatatttc cgaatacggt
    11221 ctttttatac cgtaaaccct aacatcatcc ccgtatcttc atgctccagc agatggcagt
    11281 gcgccatata agcatgttct ttcggtgcgt cgtgattaaa cttcaccagc acttcactga
    11341 cattgccttc taccttaacg gtatctttcc agcccgcgcg atgagccgct ggcggtttgc
    11401 cattttctga caagatacgg aactgcgtgc cgtgaatatg gaacggatgc agcatcatgt
    11461 cgcccacgcc agagataacc caacgttcgt actgcccttt cgccgccgca aacatcggct
    11521 tattcatatc aaacgcctga ccgttgattt tattggcatg gtggaaatcg aacttcccgc
    11581 cgtggttcat atgattcata ttaccgtgcc ccatatggcc catcatctgg ctgtgatcca
    11641 ttccggccat cgcctgatcg ccatatttct ccattagcat ctgcataccc atcatatcga
    11701 gcatcgggtc catagaaagt tgcagcttgc gtaccgtcag cccttccagc gaaggtaacg
    11761 caggcaggct acttaatgtg tctggcaaag caccggaggc actaatagca atcggctgaa
    11821 tccgcattac cggatgcggc ttgtcaaacg gcgcaatcgc catccccatc tggctgaccg
    11881 gtagcgtcac caggtcaaag ggtttgttgt cgttaacctc caccagcact tcaaaacgct
    11941 cgcccatcag caccggcagc tcgctcacct tcaccggttc aggtagcaga ccaccgtcgc
    12001 tggcaatcac atacagcggg cgattgtcgc tggtggcgaa attgagcgag cgggcattac
    12061 agccattgag caaacgcagg cgcagccaac cacgcggggc agcgtgttgt gggtagattg
    12121 caccgttggt cagcagcgta tcgccaaacc agcccacggc ggcggtcatc acatccagtt
    12181 gataatcaat ctgcccatca gcgttaaatt ttttatcctg aacaatcacc ggaacatcat
    12241 cgatacccca ctgttttggc agcattaatt tcaggatctc gtcatcttca attaccacca
    12301 gcccagccaa ccccatcgcc acctgtcgcc cagtcttgcc atgttgatgt ggatggaacc
    12361 agcaagtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca cctggcggaa
    12421 taattccctg cgggccgccg tcgacttcac ccggtacttc cagcccgtgc cagtgcaacg
    12481 tcgtctcttc cgtcagttgg ttatagatat caaccgttac cgctttgccg cgctgtaatt
    12541 tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcagtt ttctcgccaa
    12601 aggtagactg acctgcgcca atagttaact gaatgcgatt acgggcatcg gtcgtgagca
    12661 aatcagggat cggtaacgtt gggcgttccg ccgcaaagac tgcgcggctc cacagcggca
    12721 aggctgaagc cacacccagc gcgacggaat attttaagaa atcacgacgt tgcatagtta
    12781 tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg agcatagtcc ttccccttac
    12841 gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc tcgctcgcag
    12901 tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt ttttcagaac
    12961 agtgttattc ggcagcctga tggccgtttg cgcaaacagt tacgcgctca gcgagtctga
    13021 agccgaagat atggccgatt taacggcagt ttttgtcttt ctgaagaacg attgtggtta
    13081 ccagaactta cctaacgggc aaattcgtcg cgcactggtc tttttcgccc agcaaaacca
    13141 gtgggatctc agtaattacg acaccttcga catgaaagcc ctcggtgaag acagctaccg
    13201 cgatctcagc ggcattggca ttcccgtcgc taaaaagtgc aaagccttgg cccgcgattc
    13261 cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa atattgaccg
    13321 tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt ttttacgggt gttaacaaag
    13381 gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa acgctacacg accagtttgg
    13441 gcagtacttt gcggtagata acgttctgta tcatgaaaag accgatcacc aagatctgat
    13501 catttttgag aacgctgcat ttggtcgcgt aatggcgctg gatggcgtag tacaaaccac
    13561 cgagcgcgac gagtttatct atcatgagat gatgacccat gttccgctac tggcccacgg
    13621 tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt gccatgctgc gtgaagtaac
    13681 ccgacataaa aacgttgagt caatcacgat ggtggaaatc gatgcgggtg tcgtgtcgtt
    13741 ctgccgtcag tatctgccca accataacgc cggtagctac gacgatccgc gctttaagct
    13801 ggtgatcgac gatggcgtca atttcgttaa tcaaaccagc cagacctttg atgtcattat
    13861 ctccgactgc accgatccta tcggtcccgg cgaaagtctg ttcacctcag cattttatga
    13921 aggctgcaaa cgttgcctga atcctagcgg tatcttcgtc gcacaaaacg gcgtctgctt
    13981 tttacagcag gaagaagcca tcgacagcca tcgcaaactc agccattact tcagcgacgt
    14041 tggcttttat caggcggcga tcccgaccta ttacggcggt atcatgactt ttgcatgggc
    14101 gacagataac gacgccttac gccatctctc aaccgaaatt attcaggcgc gttttctcgc
    14161 ttctggcctg aaatgccgtt attacaatcc ggcaatccat acggcagctt ttgccttacc
    14221 tcagtatctg caagatgcac tggcttcaca gccgtcctaa ggagaagata agaaattgaa
    14281 aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt gtatttacga
    14341 tatctgctac gccaaaactg ccgaagagcg cgacggttat attgcttata tcgatgaact
    14401 ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta tcggggccaa
    14461 tattcttaac atcgcccgcc aggattacga accacagggt gccagcgtca ctattctggt
    14521 gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca gaacaccccg gcccactgcc
    14581 agaaacggtc gttgcccatc tcgataaaag tcatatttgc gtacatacct acccggaaag
    14641 ccatcctgaa ggtggtttat gtaccttccg cgccgatatt gaagtctcta cctgcggcgt
    14701 gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg atatcgtaac
    14761 cattgattat cgcgtgcgcg gttttacccg cgacattaac ggtatgaagc actttatcga
    14821 ccatgagatt aattcgattc agaactttat gtctgacgat atgaaggcgc tgtatgacat
    14881 ggtggatgta aacgtctatc aggaaaatat cttccatacc aagatgttgc ttaaagagtt
    14941 cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca gcgagcgcca
    15001 ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc gcaatatgcc
    15061 agctgtttaa cggctctggc ggagctccag gctccgccag atttatttac ttctgctgca
    15121 cgaaattgcg gtaagccgcc acgacctgca gaaaatcctc aacgccgcat agcgacaggc
    15181 tttcttcgtc gtagtagttc atcccctctt ccatttcatc gccagcgaat tccagttgat
    15241 tggcgcgaac catcacctct tcaccgtcca tccacagggt gtattcatgc cctgcccgtt
    15301 gccaggaccg ttcgctacct ttcagtgcgt gcgctgcttg ttccacttca tcaagcaagg
    15361 ccaggttttc tttcacctct tcattaaacc agtgcccgac cacttcatgc cccatggaca
    15421 tacgcacctt taccactccg gtaatatcgc gcagaaattc gtaatccata gtgtgttcct
    15481 ctgctcccgg caatgcgccg aaatcgcatt gcgccattga tgtaattatc gcagccttac
    15541 cggagaaaaa cagcggaacg gcgatggttt agaaataaaa aatgcccggg aatctcattc
    15601 accgggcatt gtgtcgttta tgcgcagcgc gtgcgcttac ttattacacc gcagtctgga
    15661 aaatcacccc atcggctttt tcggtgtact gagaaagttg gttgaagttc agataacgat
    15721 aagtatcaac ggcagtttta tctacttgcg caacgtaggt ctggtactct tccggcgtcg
    15781 gcagtttgcc aatcagcgcc gcaacagccg ccagttctgc agaagccagg aagacattcg
    15841 cgccagtccc cagacggttc gggaagttac gggtagaggt ggaaaccacc gtcgcaccgt
    15901 ctgctacacg cgcctggtta cccatacaca gagaacagcc cgggatctcg atacgagccc
    15961 cgctcttacc gaagacgctg tagtagcctt cttcagtcaa ctgtgcggcg tccatacggg
    16021 ttggcggtgc cacccacagg cgggtcggca actggccttt atgcgcatcc agcagtttac
    16081 ccgcagcacg gaagtgaccg atgttagtca tgcaagaacc gataaatact tcgtcgatct
    16141 tctcgccctg taccgcagac agcggacgcg cgtcatccgg gtcgttcgga gcacacagga
    16201 ttggctcttt aatatccgcc agatcgatgt caatcactgc cgcgtattcc gcatctgcat
    16261 cggcttccag cagctcagga ttcgccagcc atttttccat gccctgaata cgacgttcca
    16321 gggtacgacg atcgccgtaa ccttccgcga tcatccactt cagcaggacg atattagagt
    16381 taaggtattc gatgattggt tctttgttca gcttgatagt acaaccagcg gcagaacgct
    16441 cagcggacgc atcggtaagc tcaaaggcct gctcaacttt cagatccggc agaccttcaa
    16501 tttccagaat gcggccagag aagatgtttt tcttgccttt cttctcaacg gtcagcagac
    16561 cttgtttgat ggcatacagc ggaatggcgt gtaccagatc gcgcagggtg atgcccggct
    16621 gcattttgcc tttgaagcgc accagaacgg attccggcat atccagcggc attacgcctg
    16681 ttgcggctgc aaatgccacc agaccagaac ccgccgggaa agagataccg atcgggaaac
    16741 gggtatggga gtcacctccg gtaccgacgg tatccggcag cagcatacgg ttcagccagg
    16801 agtggattac gccgtcaccc ggacgcagcg acacaccgcc acggttcata atgaagtccg
    16861 gcagcgtgtg gtgcgtgttc acgtcaactg gcttcggata cgccgcggtg tggcagaaag
    16921 actgcatcac caggtcagcc gagaagccca ggcacgccag gtctttcagt tcatcacggg
    16981 tcatcgggcc ggtagtgtcc tgtgaaccta cagaagtcat tttcggttcg cagtacgcgc
    17041 ccggacgaat gcctttcacg ccacaggcac ggcctaccat tttctgcgcc agcgagaagc
    17101 cgcgatcgct ctcagcgaca tctttcgcct gacggaacac atcactgtgc ggcagaccaa
    17161 gtgcttcacg cgctttggtg gtcaggccac gcccgataat cagcgggata cggccgccag
    17221 cacgcacttc atcaatcagc acgtcggttt tcagttcgaa ggtcgccagc agttcgcctg
    17281 tttcgtggtt acgcacttca cctttgtacg ggtaaacgtc aatcacgtcg cccatgttca
    17341 ggttagagac gtcgacttcg attggcagtg cacccgcgtc ttccatcgtg ttaaagaaga
    17401 tgggtgcaat tttaccgccg aggcacaaac caccgccgcg tttgttcggc acatgtggaa
    17461 tatcatcgcc cataaaccac agaacggagt tcgtggcgga tttacgcgat gaacccgtac
    17521 ccacaacgtc accgacgtac gccagcggga aacctttctg ttgcagagct tcaatctgtt
    17581 tgatcgggcc aacaacacct ggctggtctg gctcaatacc ttcacgggcg tttttcagca
    17641 tcgccagcgc gtgcagtggg atatccgggc gcgaccacgc atccggtgcc ggagaaaggt
    17701 catcggtgtt ggtttcgcca gtgactttga agacggtaac ggtcagtttt tcagccagcg
    17761 ccgggcgatt cataaaccat tcggcatccg cccaggactg cattacctgc ttcgcatatt
    17821 cgttgcctgc tttcgctttc tcttctacgt catagaagtt atcgaacatc agcagtgtgt
    17881 gagaaagtgc tttggcagcg atcggtgcca gtttggcatc atccagcgcg tcgatcagcg
    17941 gatgaatgtt gtaaccaccc tgcatggtgc ccagcagttc gatggctttt tccggagtca
    18001 gcagagggga tttggcttcg cctttcgcga tagcagccag taagcctgct ttgacatagg
    18061 cggcttcatc gacgccaggg ggaacacggt tggttaacag atctaacagg aattcttctt
    18121 cgcccgcggg cgggtttttc agcagctcta caagtgcggc catttggttt gcatccaggg
    18181 gtttgggcgc aatcccctca gcggcacgct cagctacgtg cttacggtat tcttctagca
    18241 cgacggttct cctcgctctc attgtcatag tgcggcaggc gattctcttc acgctcctgt
    18301 gagacagcag tttgtagggt aaatgcccag taccgcaacg ggcagaatag caggattttg
    18361 acgacgtgtt aatctgttta caaaaaagca acataaaaaa gtggctgaat cgttaaggat
    18421 ggtctagaga ttgtttccct ccgtaaaaac ttcgttaatt cgcatggtga taatcacctt
    18481 tctcaacgaa tcccaagatt agtcaaaatt taaactaccg cctctttata ctcggattaa
    18541 cagcacctgc gggtgacagt tcgccgacca ttgcgatttc cttgagatcc gaattatgaa
    18601 aatgactttg ccgtttaaac cccatgtgct ggcactaatt tgcagtgccg ggctttgtgc
    18661 cgcctctgcc gggctatata taaaaagccg cacagtggaa gcggctgtgg aaacgcaatc
    18721 gacacaactg gctgtgtctg acgctgccgc ggttacgctt cctgcaacgg tttccgcgcc
    18781 tcccgtaaca cccgccgtcg ttaaatccgc attcagcact gcacaaatag atcaatgggt
    18841 cgcgcccgtc gcgctgtatc ccgacgccct actttcgcag gtgctgatgg catcaaccta
    18901 tccggcaaac gttgctcaag cagtgcaatg gtcgcacgat aatccactta aacaaggcga
    18961 tgctgctatt caggcggtat ctgaccagcc gtgggacgcc agcgttaaat cactggtggc
    19021 ctttccacaa ttgatggcat tgatgggcga aaacccgcaa tgggtgcaaa acctgggcga
    19081 tgcttttctg gcgcagccgc aggacgtgat ggactcggta cagcgattgc ggcaactggc
    19141 gcaacaaacc ggttcgctga agtcatcaaa cgaacaaaaa gttattacca caacgaagaa
    19201 agctgtaccg gtaaaacaga cagtcacggc acccgtcata ccatccaata ccgttttaac
    19261 tgccagcccc gtcattacag agcctgcaac aaccgtcatt tccattgagc ccgccaatcc
    19321 tgatgtggtc tatattccca actacaaccc aaccgtggtt tacgggaact gggccaatac
    19381 tgcgtatccg ccggtttatc tgccaccacc agccggagaa ccgtttattg acagctttgt
    19441 gcgcggattc ggctatagga tgggtgttgc taccacgtac gcactattca gcagcatcga
    19501 ctgggatgac gacgatcatg accatcatca tcatgacgat gatgattatc atcaccacga
    19561 tggcggtcat cgtgacggta atggctggca acacaacggc gacaacatca atatcgacgt
    19621 caacaatttc aaccgtatca ccggtgagca tcttactgat aagaatatgg catggcggca
    19681 caatccaaac taccgtaatg gtgtgcccta tcatgatcag gatatggcaa agcggtttca
    19741 tcaaaccgat gtcaacggcg gaatgagtgc cacgcagcta cctgctccaa cacgcgacag
    19801 ccagcgtcag gcggcagcaa gtcagtttca gcaacgaaca cacgccgccc ccgtcattac
    19861 acgagatacc caacgtcagg cagcggcaca gcggtttaat gaagctgaac actatgggag
    19921 ctatgacgac ttccgcgact tcagccgtcg ccaacccctg acccagcaac aaaaggacgc
    19981 cgctcgtcag cgttatcagt cagcttctcc tgagcagcgc caggcagttc gcgagagaat
    20041 gcagactaac ccgcagatcc agcagcgaag agaggcagcg cgtgagcgca ttcagcccgc
    20101 ctcgcctgag cagcgccagg cagtccgcga gaaaatgcag actaacccac agatccagca
    20161 gcgaagagac gcagcgcgtg agcgtattca gtcagcctcg cctgagcagc gccaggtgtt
    20221 taaggaaaaa gtacagcagc gcccactgaa ccaacagcaa cgtgataacg cccgccagcg
    20281 tgttcaatca gcatcacctg aacaacgtca ggtttttcgg gagagagttc aggagagccg
    20341 cccacaacgt ctaaacgaca gtaaccgtac tgccagattg aataacgatc aacggtcagc
    20401 agtacgcgaa cgtctctctg agcgcggagc aaggcgactg gaaaggtaaa ttacagacgt
    20461 aaaaaaagcg gcgtggttag ccgctttttt aattgccgga tgttccggca aacgaaaaat
    20521 tacttcttct tcgctttcgg gttcggcagg tcggtaatgc taccttcgaa cacttctgcc
    20581 gccaggccca cagactcgtg cagagtcggg tgcgcatgga tggtcagcgc gatgtcttca
    20641 gcgtcacaac ccatttcgat tgccaggccg atttcaccca gcagctcgcc gccgttggta
    20701 ccgacaatcg caccaccgat cacacggtga gattctttgt cgaaaatcag cttggtcata
    20761 ccgtctgcgc agtcggaagc gatagcacga ccagaagcag cccacgggaa ggtggcggtt
    20821 tcatagctga tgcctttctc tttcgcttct ttctcagtca gacctaccca tgcaacttct
    20881 ggttcggtat aggcgataga cgggataact ttcggatcga agtagtgttt tttaccggcg
    20941 ataacttcag cggcaacgtg accttcgtga acacctttgt gtgccagcat cggctgacca
    21001 acgatatcgc cgatagcaaa gatgtgcggt acgttggtac gcagctgttt gtcaacgcgg
    21061 atgaaaccac ggtcgtccac ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc
    21121 ggcacacgac caatcgctac cagcacggcg tcgtaacgct gcggttcagc gggtgctttt
    21181 ttgccttcca tcgtcacata aataccgtct tctttcgctt caacggcggt aactttggtt
    21241 tccagcatca ggttgaattt cttgctgata cgcttggtga agactttaac gatgtctttg
    21301 tcagctgccg ggataacctg gtcgaacatt tcaaccacgt caatctgtga acccagcgcg
    21361 tggtatacgg tgcccatttc cagaccgatg ataccgccac ccattaccag caggcgttct
    21421 ggtacttctt tcagttccag cgcgtcagtg gagtcccaga tacgcggatc ttcatgcgga
    21481 ataaacggca gttggatcgg gcgagaaccc gctgcaatga tcgcgttgtc gaagttgatc
    21541 acggttttac cgttctcacc ttcaacttcc agggtgttag ccccggtaaa tttacccaga
    21601 ccgttgacca ctttgacttt gcggcctttc gccataccag ccagaccacc ggtcagctga
    21661 ttgatcactt tctctttcca ggtacgaatc ttgtcaatat cggttttcgg ttcgccgaag
    21721 acgataccgt gttcagccag cgctttggct tcttcgataa cttttgctac gtgcagcagt
    21781 gctttagaag ggatacagcc gacgttcagg caaacaccgc caagggtgtt gtaacgttct
    21841 acgattacgg tttccagacc taaatcagcg caacggaagg cagcggagta acctgcgggg
    21901 cctgccccaa gtaccacgac ctgagttttg atttcagtac tcatcatgac ctctatatat
    21961 ttatctccgg cggtcatacc cgtcgtcttt caggccgcag cggcgttagc ttcacgccct
    22021 caccccagtc acttacttat gtaagcccct ggggattctg gcgcttgcta ccttgctgca
    22081 cccagaaatc catagggtat gtgaagtaat accctaacca ccaccgggtc gttctatccg
    22141 tcggtatttt acaaaattgt taacaatttt taaacaacaa acggcaaccg atttgtctat
    22201 tcgctaataa cctcaataca ttcatgagat taccagaaaa aagccggccg ttgggccggc
    22261 tcttttactt acatcaccag acggcgaatg tcagacagcg tgttgttaat gatggtaatg
    22321 aaacgggcac catcagcacc gtcgatcacg cggtggtcga aggagagaga aatcggcagc
    22381 atcagacgcg gcacgaactc tttaccattc cacaccggct ccatcgcgga cttggaaacg
    22441 ccgaggatag ccacttccgg cgcgttcaca atcggcgcga agtgggtagt acccaggccg
    22501 ccgatgctgg agatggtgaa gcaaccgccc tgcatttcgc ccgcagtcag cttaccgtca
    22561 cgcgctttct tagaaatagt catcagctcg cgagacagct cgatgatgcc tttcttgttg
    22621 acgtctttga ataccggaac aaccagaccg ttcggggtat ccaccgccac accgatgttg
    22681 atgtatttct tcagggtcag acgctgaccg tcttccgaca gcgaactatt gaagcgaggc
    22741 atctgctcaa gagctgcagc aacggctttc atgatgaaga caaccggggt gatcttcaca
    22801 tccagcttac gtttcgccgc ttcttcgttc tgctgtttac ggaacgcttc cagctcggtg
    22861 atatcggttt tgtcgaagtg ggtaacatgc gggatcatca cccagttacg gctcaggtta
    22921 gcaccagaaa ttttctggat acggcccagt tccacttctt cgatttcacc aaacttgctg
    22981 aagtccacct tcggccacgg cagcatgcct gggataccgc cgccagtcgc cgccggagct
    23041 gcttctgcac gtttgatagc ttctttcacg taagcctgaa cgtcttcgcg caggatacga
    23101 cctttacggc cagtgccctt cactttcgcc aggttaacac caaactcgcg tgccagacgg
    23161 cggatcagcg gagtcgcgtg aacataagcg tcgttttcag caaattcaga tttgccttcc
    23221 gctttcgcag ctggtgctgc tgccggggct tcagcttttg ctgccggtgc cggcgctgcc
    23281 gcttcctgtt tcgcaggagc tgccgcaggc gctgcgcctt caacttcgaa gatcataatc
    23341 agcgagccag ttttcacttt atcgccaacg ttgactttca gttccttcac gacgcctgca
    23401 aacggcgccg gaacttccat agaagctttg tcgccttcta cggtgatcag tgactgttca
    23461 gcggcaactt tgtcgcccac tttcaccatc acttcagtca cttcaacttc gtcaccgccg
    23521 atatccggaa cgttaacttc tttcacgcca gctgctggtg caggggccgc tgccggagcc
    23581 gcttcctgtt tagccgccgg agctgccgcg cctgcttcac ccgcgacttc gaagaccata
    23641 atcagcgagc cggtagacac tttgtcaccc acgttcactt tgatctcttt cacggtgcca
    23701 gcaaacggag ccggaacttc catagaagct ttatcgcctt ctacggtgat cagcgactgt
    23761 tcagcttcaa ctttatcgcc aactttcacc aggatttcgg tcacttcaac ttcgtcgctg
    23821 ccgatatccg gaacgttaac gtcttttgcc gccgcagccg cgggtgctgc tgccggagct
    23881 gcttctttct tctcttctgc ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg
    23941 aaaatcataa tcagtgcgcc ggtctgggtt ttatcgccaa cagagacttt gatctcttta
    24001 acgatacccg cctgcggaga cggaacttcc atagaggctt tgtcgccttc tacggtgatc
    24061 agcgactgtt cggcttcaac tttgtcgccc actttgacca ggatctcggt gatttcaact
    24121 tcatcagccc cgatgtccgg tactttgatt tcgatagcca ttattctttt acctcttacg
    24181 ccagacgcgg gttaacttta tctgcatcga tgttgaattt ggcgattgcg tcagcaacca
    24241 ctttcttatc gatttcgcca cgtttagcca gttcgcccag cgccgcaacc acgacataag
    24301 aagcatcaac ttcgaagtgg tgacgcaggt tctcacggct gtcggaacga ccgaagccat
    24361 cagtacccag tacgcggtag tcgtcagccg gtacgtaagt acggacctgc tcagcgaaca
    24421 gtttcatata gtcggtagat gccactgccg gagcgtcgtt catcacctga gcgatatacg
    24481 gaacgcgcgg agtttccagc ggatgaagca tgttccagcg ttcacaatcc tgaccatcac
    24541 gcgccagctc ggtgaaggag gtcacgctat aaacgtcaga acctacgccg taatctttcg
    24601 ccaggatctc tgccgcttca cggacgtgac gcaggataga accggagccc agcagctgaa
    24661 ctttaccttt gctaccttca atggtttcga gtttgtagat acctttacgg ataccttcct
    24721 cagcaccttc cggcattgcc ggcatgtggt agttttcgtt cagcgtggtg atgtagtagt
    24781 aaacgttctc ttgtttttca ccgtacatac gctccagacc gtcatgcata atgacagcaa
    24841 cttcgtaagc gtaagccggg tcgtaagaga tacagttcgg aatagtcagc gactgaatgt
    24901 ggctgtgacc atcttcatgc tgcagacctt ctccgttcag ggtggtacga ccggaagtac
    24961 cgccgatcag gaagccacgc gcttgctggt cgccagccgc ccagcacaga tcgccaatac
    25021 gctggaagcc gaacatcgag taatagatgt agaacgggat catcggcaga ttgttggtgc
    25081 tgtaagaggt cgccgctgcc agccaggaac aacctgcgcc cagctcgttg atcccttcct
    25141 gcagaatctg acctttctcg tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg
    25201 tgtactgctg accgttcggg ctgtaaatac caatctgacg gaacagacct tccataccga
    25261 aagtacgcgc ttcgtcggcg atgatcggta ccagacgatc tttgatcgac ttgttcttca
    25321 gcattacgtt cagggcacga acgaaagcga tagtggtaga gatctctttg ctctgctctt
    25381 ccagcagcgc gccgaagtct tgcaggctcg gcagctcaag cttctcggtg aagttcggct
    25441 gacggcttgg cagataaccg tgcagtttct gacgctgtgc gtgcagatag gtatgctctt
    25501 cagaaccttc cgggaaggtg atgtacggca gtttttcgat atctgcatca gacaccggca
    25561 cattgaaacg gtcgcggatg tgacgcacgc cgtccatgtt cattttctta acctggtgcg
    25621 cgatgttttt accttcagcc gcgtcgccca tgccgtaacc tttaatggaa tgagcaagga
    25681 ttactgtcgc tttgcctttg gtttcctgcg ctttcttgaa tgcagcgtag attttcttcg
    25741 gatcgtgacc accacggttc agtgcccaga tctgctcgtc agtccagtct gcaaccagtg
    25801 ctgcggtttc aggatattta ccgaagaagt gttcacgaac gtacgcacca tctttcgatt
    25861 tgaaggtctg gtagtcgccg tcaacggttt cgttcatcag ctggatcagt ttaccgctgg
    25921 tatctttacg cagcagttca tcccaacggc taccccacat cactttgatc acgttccagc
    25981 cagcaccttc gaagatgcct tccagttcgt tgatgatctt gccgttaccg gtgaccgggc
    26041 cgtcaagacg ctgcaggtta cagttgataa cgaagaccag gttatccagt ttttcacggg
    26101 tagcgatggt gatcgcacct ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg
    26161 cgtaaacggt ctgtttagag gtatctttca ggccacggtg ttccagatat ttcaggaatt
    26221 tagcctggta aatagcacca atcggcccca gccccataga tacggtcggg aactgccaga
    26281 attccggcat cagtttcggg tgcggatagg aagagaggcc attgccgtga acttcctgac
    26341 ggaagttatc cagctgctcc tgagtcagac gaccttccag gaaagcacga gcgtacacgc
    26401 ccggggagat gtggccctgg aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac
    26461 ggaagaagtg gttaaagcac acatcataaa tggttgcgga agactggaag gacgccatat
    26521 ggccgcccag ttcgaggtct tttttcgacg cacgcagcac cgtcatgatg gcgttccagc
    26581 ggatagctga acgaatacgg cgttccagtt ccagattacc cggatactcc ggttgttctt
    26641 caacggggat ggtgttgatg tagttgctga tacctgtgcc tgcggctacg tttacaccgc
    26701 ctttgcgggc ttcagcaagc agttggtcga tcagatactg agcacgctca acaccttctt
    26761 cacggatgac cgattcgatc gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat
    26821 ttgggaaacg ttctgacatg ggttattcct tatctatcta ataacgttga gttttctgga
    26881 acctgtccca ttgaactctc gccggaaagc tcaataagac aggttctacg tttagttgcc
    26941 gcgcttttat atgcgcttga tttacaacat cttctggata atttttacca gtaaaatcac
    27001 taattctttc gttgctccag acgacgcaga gaacgctcac ggcggctctc ttcacgactt
    27061 ctgtcgagca aaatttcttc gataaaggcc agatggcgat gcgatgcttc gcgcgcttct
    27121 tccggcttac cggccataat cgcttcaaat atgcgggtgc ggtgactact caccagcggc
    27181 agcatctcgc gacgcgaata gagcaattcg aagttctggc ggacattctg ggccaacatc
    27241 ggctccatac accttagcag atgaagcaga accacattgt gggccgcttc agtgacagca
    27301 atctgatact ggagtacggc gtttgattcc gcgtccagat cgccagactg ctgcgccagt
    27361 tctatggcgt gatggagttc acggatgcgt tccttgtctt catcggtact acgcagcgcg
    27421 gcgtaataag cggcgatacc ttccagggcg tgtcgtgttt cgagcaagtc atactgtgac
    27481 tcaggatggt cggagagcag ctccaccagc ggatcgctga agctttgcca taggctgctc
    27541 tggacaaaag tgccgccacc ctggcgacga agcaacaagc ccttcgcttc gagacgttga
    27601 atcgcctcac gcaaggaggg acgggagacg tcaaactgtt ttgccagttc gcgttccggt
    27661 gggagttttt cgcccgggcg gagagtgcct tcgaggatca aaaactccag ttgctgctca
    27721 atcacatcgg agagttttgg ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag
    27781 ccacttgccg aagtcaattg gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag
    27841 tattcacctt atgtccatac aggttttgat tgaaatcatg aaactgtgca cattttaaca
    27901 acttgacata tataacgttt caaagttgta actatgcaca aatgtagact ttacgtagga
    27961 aagggatttt ttaacgatta ttaaactata aaaatccgaa ttgaaccgat tcacttacca
    28021 attttgtgat ttttaattca attaaaacga atttaaattc attctacata ttgagagggg
    28081 ttgaggctga gctttacaaa cggtttcttt ttaagcaact catcttcaac catgcataaa
    28141 gcgggtgcat tcgctgccgc ataccattat tcttgatctg acggaagtct ttttgtaaca
    28201 attcaaactt ctttgatgta aacaaattaa tacaacaaac ggaattgcaa acttacacac
    28261 gcatcactgc gtagatcaaa aaaacaacca ccgcacgagg tttcatgatg gaaggtcaac
    28321 agcacggcga gcagctaaag cgcggcctta aaaaccgcca tattcagctt atcgcgctgg
    28381 gtggcgcgat agggaccggg ttattcctgg gtagcgcctc cgtaatacag tccgcagggc
    28441 cagggattat cctgggttac gccattgctg gttttatcgc ctttctgatc atgcgtcagc
    28501 tgggtgaaat ggtggtcgaa gaacctgtcg caggctcctt tagccacttt gcttataaat
    28561 actggggcag ttttgccggt ttcgcctctg gctggaacta ctgggtactg tacgttttag
    28621 ttgccatggc tgagctgact gccgtgggta aatacattca gttctggtat ccggaaatcc
    28681 ccacctgggt ttctgccgcc gtattctttg tggtgattaa cgccatcaac ctgaccaacg
    28741 ttaaagtgtt tggcgagatg gagttctggt ttgccattat caaagttatc gctgtggtag
    28801 cgatgatcat cttcggcggc tggctgctat tcagtggcaa cagcggcccg caggcgaccg
    28861 ttagcaacct gtgggatcag ggtggtttcc tgccgcacgg cttcaccggg ctggtgatga
    28921 tgatggcgat tatcatgttc tcgttcggtg gtctggaact ggtggggatc accgcagcag
    28981 aagctgataa cccggagcaa agtataccga aagcaactaa ccaggttatc taccgcatcc
    29041 tgattttcta tattggttcg ttagccgttc tgctctcact gatgccgtgg acccgcgtta
    29101 ccgccgatac cagtccgttt gtgctgatct tccacgagtt aggcgatacc tttgtggcga
    29161 atgcgctgaa catcgtggta ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt
    29221 gcaacagccg tatgctgttt ggtctggcac aacagggtaa tgcgccaaaa gcgctggcgt
    29281 ctgtcgataa acgtggtgta ccagtaaata ccattctggt gtctgcactg gtaacggcgt
    29341 tgtgcgtact gattaactac cttgccccag agtccgcttt cggactgtta atggcgctgg
    29401 tggtatctgc actggtaatc aactgggcga tgattagcct ggcgcatatg aaattccgtc
    29461 gcgccaagca ggaacaaggc gtggtaactc gcttccctgc tctgctttat ccgctgggta
    29521 actggatctg cctgctgttt atggcggcgg tactggtgat tatgctgatg accccaggaa
    29581 tggcgatttc ggtatacctg atcccggtat ggctgatcgt gttaggtatc ggctatctgt
    29641 ttaaagagaa aaccgccaaa gccgtaaaag cgcattaatc tctctacgcc ctcacccgta
    29701 cagggtgagg gcaataatct ttacaccaac gccccgtaaa tcgtcagtag tgcaatcacc
    29761 accacgacca cgaacgaggt tttcttcgcc attgaaaccg ctgccttcgg cgtctccacc
    29821 ttatcgacat gcggttcacg cgccagagag aactgcgcca gacgcgttaa cacctgatac
    29881 tgcgaagtat ggaagtcacc cagcgaagca aaccaggccg gtaacgcttt ctccccatga
    29941 ccgatcaagg catataccac acccgcaaga cgaaccggca cccaatccag tacatgaagc
    30001 acggcatcaa tgccggactg taaacgatga tgcggcgtct ggtatcgcgc cagccagtat
    30061 tgccatgcac gcaaaaacgc ataccccatc agcgtaacgg gtccccagat tccccccaca
    30121 atcaaccaga acagcggagc aagataaaaa cgaaagttaa tccacagcaa tgcattttgc
    30181 agctcacgca aatactcacg ttcatcgcag cctgccggga cgccgtgaat catggtgagt
    30241 tcgccagcca tcgtgacacg ggcatggcta tcattacgtg aagcagctgt cagataagca
    30301 tgataatgaa gacgaacttt acctgcgcca atacacagca aaccaatcag cagccacacc
    30361 agcagcgtgg gaacgttgaa caatactccc tgcaatgcgc gtaacagtaa aaaagtcacg
    30421 cccatcgcaa taatggtcat gcctaacgtg cgcccgagag aaaaatgttt cacccgccga
    30481 aagaacgctt caagacgatg atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg
    30541 aaaattaaca ccagtaaggt tgtaaatagc gtcatgttgt ctccttgctg accagcgcac
    30601 gaaaccgcgc ccaatcaaat gcagggccgg gatcggtttt ccgatccggc gcaatatcac
    30661 aatggcccgt catgttatta gcgatatctg gatagcgatc aatcagtgcc cgcgtaaccg
    30721 ccgcaagctg ttgatactgc gcatcggtat acgccagcgt atcggtgcct tcaagctcaa
    30781 tcccaataga aaaatcattg cagcgttcgc gcccctgata ctgagagact cccgcatgcc
    30841 atgcacgttt atcgaaagga acatactgga ctatttcacc atcacggcga atcaaacagt
    30901 gagcggagac gcgcaaatgg gcgatctcag caaagaaagg atgtgcctgc ggatcaatag
    30961 ttccagtgaa taatgcgtcg atccacggac cgccaaactc gcctggcggc aggctaatat
    31021 tgtgcaccac cagcagggtg ggtgtttcgt catccgggcg gcaatcgtaa tgtggtgagg
    31081 gaacgcggcg cgcgccaacc agccacccct gttctaacaa catgcaggat ctccttatat
    31141 gtggtgctaa tacccggttc agagtagcat gtttctacct tatgattcgt tagctatctg
    31201 gagttttaac atgccgcctc gccgctataa ccctgacacc cgacgtgacg agctgctgga
    31261 acgcattaat ctcgatatcc ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg
    31321 aacagtcgat gccaacaatg acattacggc aaaactttta ccggaaaact ctcgctctca
    31381 tgccacggtg atcacccgcg agaatggcgt attttgcggc aaacgctggg ttgaagaggt
    31441 gtttattcaa ctggcaggcg acgatgtcac cataatctgg catgtggatg acggcgatgt
    31501 catcaacgcc aatcaaccct tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg
    31561 cgaacgcact gcgcttaatt ttgtgcaaac cctttcagga gttgccagta aggtacgcca
    31621 ctatgtcgaa ttactggaag gcaccaacac gcagttgttg gatacgcgca aaaccttacc
    31681 tggcctgcgt tcagctctga aatacgcggt actttgcggc ggcggagcga atcaccgtct
    31741 ggggctttct gatgccttcc tgatcaaaga aaatcatatt attgcctccg gctcagtgcg
    31801 ccaggccgtc gaaaaagcgt cctggctgca cccggatgcg ccagtagaag tcgaagtaga
    31861 gaatctggaa gaacttgatg aagccctgaa agcaggagcc gatatcatca tgctggataa
    31921 cttcgaaaca gaacagatgc gcgaagccgt caaacgcacc aacggcaagg cgctactgga
    31981 agtgtctggc aacgtcactg acaaaacact gcgcgaattt gccgaaacgg gcgtggactt
    32041 tatctccgtc ggtgcgctaa ctaaacacgt acaagcactc gacctttcaa tgcgttttcg
    32101 ctaattgctg acagatttat ccgctccatc gcgggcggat aatctcccct tccccaactt
    32161 tcttcgtaac gcctcgcaaa tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa
    32221 gctgctttcc cgtttcgttt tttgcccctc gcttgtgctg atggcgtttg ccaaagtagc
    32281 gccaaccaaa tcaaggagcg aaacagatgg acaagcaacg cggttttaca cttatcgaac
    32341 tgatggtggt tattggcatc attgccattt taagcgccat tggtattccc gcttatcaaa
    32401 actacctgcg caaagctgca ct
//
LOCUS       DAJTOR010000029        31372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTOR010000029 DAJTOR010000000
VERSION     DAJTOR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.029"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(15..3296)
                     /locus_tag="OO249_001189"
     CDS             complement(15..3296)
                     /locus_tag="OO249_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5635559.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISCYKNVPVTTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLVIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            3414..4607
                     /locus_tag="OO249_001190"
     CDS             3414..4607
                     /locus_tag="OO249_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5635560.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4671..5924)
                     /gene="glyA"
                     /locus_tag="OO249_001191"
     CDS             complement(4671..5924)
                     /gene="glyA"
                     /locus_tag="OO249_001191"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5635561.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6252..7442
                     /gene="hmpA"
                     /locus_tag="OO249_001192"
     CDS             6252..7442
                     /gene="hmpA"
                     /locus_tag="OO249_001192"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5635562.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIYYECFGP
                     HKVL"
     gene            complement(7487..7825)
                     /gene="glnB"
                     /locus_tag="OO249_001193"
     CDS             complement(7487..7825)
                     /gene="glnB"
                     /locus_tag="OO249_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5635563.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7886..9220)
                     /gene="glrR"
                     /locus_tag="OO249_001194"
     CDS             complement(7886..9220)
                     /gene="glrR"
                     /locus_tag="OO249_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5635564.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9210..9923)
                     /gene="qseG"
                     /locus_tag="OO249_001195"
     CDS             complement(9210..9923)
                     /gene="qseG"
                     /locus_tag="OO249_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5635565.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGCWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10088..11515)
                     /gene="qseE"
                     /locus_tag="OO249_001196"
     CDS             complement(10088..11515)
                     /gene="qseE"
                     /locus_tag="OO249_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5635566.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12073..15960)
                     /gene="purL"
                     /locus_tag="OO249_001197"
                     /gene_synonym="purI"
     CDS             complement(12073..15960)
                     /gene="purL"
                     /locus_tag="OO249_001197"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5635567.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16220..17776
                     /gene="mltF"
                     /locus_tag="OO249_001198"
     CDS             16220..17776
                     /gene="mltF"
                     /locus_tag="OO249_001198"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5635568.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLCNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17773..18309)
                     /gene="tadA"
                     /locus_tag="OO249_001199"
     CDS             complement(17773..18309)
                     /gene="tadA"
                     /locus_tag="OO249_001199"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5635569.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18334..18969)
                     /gene="yfhb"
                     /locus_tag="OO249_001200"
     CDS             complement(18334..18969)
                     /gene="yfhb"
                     /locus_tag="OO249_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5635570.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDKPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19178..20026
                     /locus_tag="OO249_001201"
     CDS             19178..20026
                     /locus_tag="OO249_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5635571.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            20082..20342
                     /gene="yfhL"
                     /locus_tag="OO249_001202"
     CDS             20082..20342
                     /gene="yfhL"
                     /locus_tag="OO249_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5635572.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20536..20616)
                     /gene="shoB"
                     /locus_tag="OO249_001203"
     CDS             complement(20536..20616)
                     /gene="shoB"
                     /locus_tag="OO249_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5635573.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21036..21416)
                     /locus_tag="OO249_001204"
     CDS             complement(21036..21416)
                     /locus_tag="OO249_001204"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5635574.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21416..22147)
                     /gene="pdxJ"
                     /locus_tag="OO249_001205"
     CDS             complement(21416..22147)
                     /gene="pdxJ"
                     /locus_tag="OO249_001205"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5635575.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22159..22887)
                     /gene="recO"
                     /locus_tag="OO249_001206"
     CDS             complement(22159..22887)
                     /gene="recO"
                     /locus_tag="OO249_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5635576.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(22899..23804)
                     /gene="era"
                     /locus_tag="OO249_001207"
     CDS             complement(22899..23804)
                     /gene="era"
                     /locus_tag="OO249_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5635577.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(23801..24481)
                     /gene="rnc"
                     /locus_tag="OO249_001208"
     CDS             complement(23801..24481)
                     /gene="rnc"
                     /locus_tag="OO249_001208"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5635578.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24754..25728)
                     /gene="lepB"
                     /locus_tag="OO249_001209"
     CDS             complement(24754..25728)
                     /gene="lepB"
                     /locus_tag="OO249_001209"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5635579.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25744..27543)
                     /gene="lepA"
                     /locus_tag="OO249_001210"
     CDS             complement(25744..27543)
                     /gene="lepA"
                     /locus_tag="OO249_001210"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5635580.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27741..28220)
                     /gene="rseC"
                     /locus_tag="OO249_001211"
     CDS             complement(27741..28220)
                     /gene="rseC"
                     /locus_tag="OO249_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5635581.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28217..29173)
                     /gene="rseB"
                     /locus_tag="OO249_001212"
     CDS             complement(28217..29173)
                     /gene="rseB"
                     /locus_tag="OO249_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5635582.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29173..29823)
                     /gene="rseA"
                     /locus_tag="OO249_001213"
     CDS             complement(29173..29823)
                     /gene="rseA"
                     /locus_tag="OO249_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5635583.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(29856..30431)
                     /gene="rpoE"
                     /locus_tag="OO249_001214"
     CDS             complement(29856..30431)
                     /gene="rpoE"
                     /locus_tag="OO249_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5635584.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30428..30583)
                     /gene="rseD"
                     /locus_tag="OO249_001215"
     CDS             complement(30428..30583)
                     /gene="rseD"
                     /locus_tag="OO249_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5635585.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            30839..>31372
                     /locus_tag="OO249_001216"
     CDS             30839..>31372
                     /locus_tag="OO249_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5635586.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRR"
BASE COUNT         7502 a         8402 c         7919 g         7549 t
ORIGIN      
        1 acagttaata gggtttagtg aacgtagtta aaaataaaag gcatcacagt ggtgaataat
       61 gccgctttcg gccaggccgg attaatcgcc gtcaattcag cgcgagcaga ctcatattgc
      121 gcaacctccc cgagtcccgc ggacgccagc atcgccacca tcaggcattt ttctttatgc
      181 tgctgttgta aatcgccata cagcgataac aggtcaggtt gtgaaacagc aaagaaatcc
      241 gcctcaatac tggttttcgc catctcttgc gcccactgtt tcatttcact aaacagttgc
      301 tgtgcggttt gctgttcacc cagcagtcgc agcgccattc cttgccagaa gagataatca
      361 accggctgat cgttgtaata actgtggatg ttaatggtgc gatcgccggt cgccgccaga
      421 cgtaaacaac gcatcgcttc agtttcatcg ccctgcgcgt tggcgcatat cgcctgccag
      481 aaccagatgt cgttatcagt ttgccccggt aaacggcctt cgcttaaatt ctccggataa
      541 tgcagcgcgg catgaagcag ttcgctggcc tgctgcggct ctctggcatc aagatgctgc
      601 caggcgcgta ataactggtt gaggataaac tgactggtga ccttcccttc cccgccttcc
      661 cacgggtgga atttacgcgt ggcgagaatg tccgccgctt tgtctgcctg acccgtgaga
      721 tgccacaaat tgagcagttc tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta
      781 ttttccagac gcgccagtcg tttctccggt gtggctccac ttaacttatc aagcaaatcc
      841 cgttcgaaaa gcagacgtgc atcctgcggc gcaagctgat aagcattatc aagataacgc
      901 gcggccagct cataatcgtg ttgcttattc cacgcatgga tcgctaaccc gcgccagccg
      961 tcggcaaact ccggcgacat ctctacgcaa cgttgccaaa aggcaatggc tttgttgtag
     1021 ctacgtttgt tgtagtagaa gcaggccagt aaatggcgag caaaccagca ctcttcaata
     1081 ctctccagcg ccgccacttc ttccagcgta ttcgggaaac ggacaaactg cgggaagaca
     1141 tcaatggctt ttgcgaccag ttcgccacgt tcggctttcg gcagcaagct ggcttgcagg
     1201 taaagcggca gcgtgcgctg gcagtccagt gcgttcagca tctctgccgc cagggtgggc
     1261 attccccagt taatcagttg cccggcggtc atcagggcgt taacgtcgcg tccctgacac
     1321 agaccgcgcc actgcgcgag agctgattcg ctacgaccat cgaaccagtt cagccaccac
     1381 agagtggcgt tcagcggata atcgcgcagc agtttctcgc gctgcacacg cgcgttgtcc
     1441 tgacgaccac ttaacaccag cagcagatta tgcaggcaaa gcacttcctg attggttggg
     1501 caggcgcgaa gactttgttg gcaaaaatcc agaccagcgt cgaagttacc attacgcgcc
     1561 gccagtcgtg ccaggccata atagccaccg gctttgctgt tgccgctcca gaccgcacgc
     1621 cagaaatcct cttcggcttg ttgatattgt ccctgacgtt cgtaagcact ggcgcgaatc
     1681 aaactcgcct gtccgcactg cggatttttg ttcagcgcat gtgcgcgttt cagagcctga
     1741 gtggcatacg ccaccgcttg cgggaaatcg gcgcggttat attccagcat cgccagcgcc
     1801 aggttacagc ggtaatccag cggatccagc gccacgccgc gcaggtagta atcgaacggt
     1861 gaacggctgg cgtgatgata ttgctccaga tgctgaccga taaaccaggc ttcatctgta
     1921 ctggtaatgt cttgtgctgc cagtggcgct ttggcgacgt ccagcaacgg caactcttgc
     1981 ggctgatgtt cctgataact cagtacaata ttgccgtcgg catcggagag ctcaatggtc
     2041 agcctttcag gattgatacc gtgcaacacg ccctggatgg cggtcgcagg catcagtgcc
     2101 acggcatcat caagtaacgc gttgcatttg ccgatttcgc ggatcaccag gcggtatccg
     2161 ttcaacggag agatggcata cagcccccac tcaatccccc gcttactacg ctggagtttt
     2221 atcaccgcat cgcgggaggc attttgcacc atgcccaaag aatgataagg caggaaatac
     2281 tgctcaaaac gcttctcttc gtaagcatca agccaggtaa aatcgggctg gttatcggca
     2341 aaaataccgg tcatcagttc gatatacggg ccgttattgt cggtcagact cttatcccac
     2401 gcctggccaa attcactgtg tccccaactc cactgttttt tacctggcgc aatatggtgg
     2461 ttggcaacgt gcagcaaacc gccatcttca tcgtgacacc acgcgccgac aaaatcgtac
     2521 tgcgattttt cggccatata tgaggttgta acaggcacat ttttatagca agaaatgtcc
     2581 actccggcgg agtagtccac tttgtagtaa gtgccggtgg cgatggggaa agcggagacg
     2641 gcccgtttgc cgtgatcaaa caccgcagtc acatccggtg ggaaaacgct ctgatgccct
     2701 tccccccctt tcactgccgg gttggcccac cacaagaaat gacgcggcgt ggcgttaccg
     2761 ttatagacgc ggctggcgat ttccagcgcc gcccggtcag ggcgcagggt gaaacctgtc
     2821 atcacctgta aaccatgcat cggctccgtt tcgcctaccc acaccgtctg tgcaccgtct
     2881 tcatgggctt cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg atgttgcggc
     2941 cagttaaact caatcccacc ggaaatccac ggccccagca gccccaccag cgcaggttta
     3001 atgacttcat tgtgataaac aaaatcgcgc tgtttcactt tatcccacgc gcgatgcacc
     3061 cgaccgccca gttccggcag gatcatcact ttgatgtagt cgttttccag ccacaccgcc
     3121 tgccaggatt tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta gggataaacc
     3181 gcgccggacg atccctgata aacgcgattt tccaggaaca tgggatgtat atcctgcggc
     3241 ccggtttcat aggtcgggat ctcaacgcgc tcttgccaca cttttactgg agtcatggtg
     3301 ccctcaatat taacaagaca tactgaatta aaagatttgt ggcagtgtat tgaacaatct
     3361 ggcaatgttt tcgcggaata atcacgcaat taactaaaca aggtttagtg aagatgagag
     3421 cctgcattaa caatcaacag attcgccacc ataacaaatg cgtgattctg gaactgctgt
     3481 accggcaaaa gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg
     3541 cagtcagtaa tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat attgacgatg
     3601 aaagccagac gcgcgggcat agtagcggta catggctgat tgcgccggaa ggtgactgga
     3661 cgctgtgcct gaacgtgacg cccaccagta ttgagtgtca ggttgctaat gcttgtttaa
     3721 gtccgaaagg tgaatttgag tatttacaga ttgatgcacc gacgccgcag gcgctgctgt
     3781 ccgaaatcga aaaatgctgg catcgccacc gtaaattgtg gccggaccgc accatcaatc
     3841 tggcgctggc aatccacggt caggttgatc cggtgaccgg cgtgtcgcaa accatgccgc
     3901 aagcgccgtg gacaacgccg gttgaggtga agtatctgct ggaagagaag ctgggcattc
     3961 gggtgatggt cgataatgac tgcgtgatgc tggcgctggc ggagaaatgg caaaataatt
     4021 cgcaggaacg ggatttctgc gtgatcaacg ttgattacgg cattggctcg tcgttcgtga
     4081 ttaacgagca aatttatcgc ggcagtttgt atggtagcgg acaaattggt cacaccatcg
     4141 ttaatccgga tggcgtcgtc tgcgactgtg gacgttacgg ctgcctggaa accgtcgcct
     4201 cgttaagtgc attaaaaaaa caggcgcggg tatggctaaa atcacaaccg gttaatactc
     4261 agcttgatcc tgaaaaactg acaaccgcgc agttaatcgc tgcctggcag agtggagaac
     4321 cgtggatcac cagctgggtt gatcgctctg ccaatgccat tggtttgagt ctgtataact
     4381 tcctcaacat cctcaatatt aatcagattt ggttgtacgg tcgcagttgt gcctttggtg
     4441 agaactggct taatactatt attcgccaga caggatttaa cccgttcgac cgcgacgaag
     4501 gaccgagcgt gaaagcgacg caaattggct tcgggcaatt aagccgcgca caacaggtgc
     4561 tgggaattgg ctatttgtat gttgaggcgc agttacgaca gatttgatgg cgcgataacg
     4621 tagaaaggct tcccgaagga agcctttttt caacagatta accgtttcgc ttatgcgtaa
     4681 accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat aacggcttca
     4741 tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc ttctgcttct
     4801 ttgaagccgc gacgcgtaat tgccggagta cccacgcgaa taccggaggt cacaaacggg
     4861 ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg gcccagagcg
     4921 gcgtctgctt ctttaccggt caggttttta tcaaccagat caaccaggaa caggtggtta
     4981 tcagtgccgc cggaaaccac tttgtagccg cgctcgagga acacttctac catcgcttta
     5041 gcgtttttcg cgacctgctg ctggtaagtt ttgaactcag gctccatcgc ttctttcaga
     5101 gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc agggaaaacg
     5161 gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaggat caggccgccg
     5221 cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg aggaaccggg
     5281 ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac gaacaggtaa
     5341 gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt ccaccacgcc ggaatatgca
     5401 gagaaaccac cgataatcat tttcggcttg tgttctttgg cttgtttttc cagatcggcg
     5461 tagtcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag tttaccggag
     5521 aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat acccagaacg
     5581 gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga gccggagtgc
     5641 ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat cgccagttgt
     5701 tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata accttcagca
     5761 tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt gtagttttcg
     5821 gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat agcctgccac
     5881 agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat ctcctgactc
     5941 agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg cgaacagtat
     6001 aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac gattaccttc
     6061 aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg
     6121 tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt
     6181 acattgcagg gctatttttt ataagatgca tttgaaatac atcaattaag atgcaaaaaa
     6241 aggaagacca tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac
     6301 tggtggaaac ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc
     6361 cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc
     6421 tgtttaacgc tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag
     6481 cggtagaaaa aatcgcgcag aagcacacca gcttccagat caaaccggaa cagtacaaca
     6541 tcgtcggtga acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc
     6601 tggacgcgtg gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg
     6661 aaatctataa cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca
     6721 ttgtggctaa aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg
     6781 gtggcgcagt ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag
     6841 gtttcccgca tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct
     6901 atcgtattgc ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg
     6961 ccaatgttgg cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt atggctgtcg
     7021 cagatgacac accagtgacg ttaatctctt ccggtgttgg tcaaacgcca atgctggcaa
     7081 tgctcgacac gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag
     7141 aaaatggcga tgttcacgcc tttgccgatg aagttaagga actggggcag tcactgccgc
     7201 gctttaccgc gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt
     7261 ttgatagcga aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa
     7321 tgcagttcta tctctgcggc ccggttggct tcatgcagtt taccgcgaaa cagttagtgg
     7381 atctgggcgt gaagcaggaa aacatttatt acgaatgctt tggcccgcat aaggtgctgt
     7441 aatttgatgt tgccggatgg aaacatccgg caacccttga cgcggtttaa attgccgcgt
     7501 cgtcctcttc accggtacgg atgcgaatga cccgtgccac gtcaaagacg aagattttac
     7561 cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa
     7621 tgtcgtccgg tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg
     7681 cgccgcggta cagctcggta tggcctttct ggcgaccaaa gcctttcact tcggtcaccg
     7741 tcatgccggt aataccgact tcggccagtg cttcgcggac atcgtccagc ttgaagggtt
     7801 ttataatcgc atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt cttttgatct
     7861 gctaaacgta acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc
     7921 gggaaagcag tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga
     7981 cgttgccttt ggtaatttgc agcagcttac gcaaatagtt gagttcaaac tgattacgtg
     8041 cctcaacaaa ggttggcagc gccgtgtttt caccctccag cgcctgctct actaatgcat
     8101 cgctaataac cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc
     8161 gcacattacc cggccagcta gcggtcatca ggcgtttcat cgcatcagtg gagaacgcgc
     8221 ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agaagcggaa
     8281 tgtcttctgt gcgctctgcc agagccggaa ttttcaggct gacaacgttg aggcggtaat
     8341 agagatcttc gcggaattcc ccgcgcgcca tcgcttttgg cagatcacgg tgagtggcag
     8401 aaataatccg cacattgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct
     8461 cctgcaacac gcgcagcagt ttgacctgta acggcgcagg catatcgcca atttcatcga
     8521 gaaatagcgt tccgccttcc gccgcctgga ataaaccttc gcgattgctg acagcgccag
     8581 taaacgcgcc acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcgc
     8641 cacagttaat agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg atagcctggg
     8701 cgaaaatctc tttcccggtg ccgctctgac cgttaatcaa aacgctgacg tctgattgcg
     8761 ccaccagccg cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaattgcct
     8821 cgcgccagcg ttcatcggtg gctggcgcgg attgctccag cgcatcgtca attgcctgat
     8881 atagcgcgtc tttgtcgaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag
     8941 caacggcatc ggggatagaa ccatgcgcag taagaataat tactggcatt cccggctgca
     9001 ctttctggat ttcagcaaat agctgcatac cgtccatttc atccatccgc aggtcgctga
     9061 taactaaatc tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg
     9121 tgaccacact gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat
     9181 cgtcatcgac caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg
     9241 gatggcgcgg gcttttcact ctcgtgcggc gtatccggcg agaaatttcc agccggtttg
     9301 cgggtcgaga gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc
     9361 agttgctgct gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct gtcgctggac
     9421 tgctggagtt tactgtaacg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg
     9481 tcgcgccaga gttgatacag tgggcgaacc tgtgccggga tctcggtact taacgcttca
     9541 atacgcgcga ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc
     9601 ccttgtttaa aggtattttg ccagcagccg tcgtcatatt ggcgagcttg ctgacgcgac
     9661 tgcgcaggca ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt
     9721 tcggttgatt taccttgcag cgcccagatg tcgctacatt cagtagaaag atagtcagcc
     9781 agttgataaa ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc
     9841 tgcacgcaac ccagcaatgc cagacatggc agccctgcca gccacagccg tcggggcaat
     9901 aatcgttgaa aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat
     9961 tgttgcagac agtctggaca tggacagcgc ggagaaaccg gagcgtacat atcgtacgtg
    10021 aagatttcga gcactgcccg gagccgaact gacaaataaa atagcctgat aggataggct
    10081 gttagcatta tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc
    10141 cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc
    10201 ttaatcccag accgctgccc ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg
    10261 gttcgaagat catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa
    10321 tataaacccg cgcaccatgt gaactgctgc gaaggcaaat gttaccggat tcagccccgt
    10381 agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca
    10441 ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc
    10501 tatgagcaga aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg
    10561 cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc
    10621 ggctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa
    10681 cctggtcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat
    10741 gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta
    10801 accaaagaat acgttgccca accgagcgta actcgctcgg tccactgaac gaaacgctat
    10861 tgcccagaga acgcccttcc cccagacggt tgatcatgcg ctcgatattt ttcaccggcc
    10921 cgataatcat ccgcgtgaaa agtagcacca tcaccagact caccagaaat agcaccagcg
    10981 attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag
    11041 agaacaccac tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca aaggcttcaa
    11101 gacgcgcggc ggcggcagca tcgggaccac tgttgttaca ctgaagttgg gccagattgt
    11161 tcaaatcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat
    11221 cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt
    11281 ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg gtcatcgctt
    11341 cactacgccg ggcatcaata agcgtagtgc ggttaaccag cgccgcctga tcgctaagcg
    11401 cattcaggct ttgccaggct tgccatgcca gcaccaacag gggtagtaga attagcaaaa
    11461 atgccagcat taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt
    11521 tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag
    11581 ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga
    11641 tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat
    11701 tcgaatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactgct
    11761 tactattatg cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg
    11821 ttgctttgag ggatttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct
    11881 aaaaagagac agcttaagat aaacttatta gattacaata aaatcaatca cttaagtgtc
    11941 gccagaaagc gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc
    12001 ggagttggga gcttatgata gtggttggtg cttaatgccg catccgggct gcaaaaccaa
    12061 tgaactgatg aattacccca actgcttacg cgcattgcgg aaaatgcgca tccatgggcc
    12121 atcctcgccc cagttttccg gatgccagga gttgctgaca gtacggaaaa cacgttccgg
    12181 gtgcggcatc ataatggtga ctcgaccact ttcagtcgtg actgccgtaa taccgttcgg
    12241 tgaaccgttc gggttggccg ggtaggtttc agtgactttg ccgaagttat cgacatagcg
    12301 cagtgccacc agccctttgc tttccagtgc cgccagatgc gccgcatcac gcacttccac
    12361 gcgcccttca ccatgagaga cagcaatcgg catttgcgag cccaccatcc cctgcaacaa
    12421 cagagacggg ctttgggtta cttcaaccag gctgaaacgc gcttcaaagc gatcggaggt
    12481 attgcgcaca aaacgtggcc acaactcact acctgggatc agttcgcgca ggttagacat
    12541 catctggcaa ccgttacata cccccagcgc cagcgtttgc ggacggtgga agaaggttgc
    12601 aaactcatcg cgtacacggt cattgaacag gattgacttc gcccaacctt caccggcacc
    12661 cagcacatca ccgtaggaga aaccaccgca cgcgaccagg gcgtggaaat cttccaggcc
    12721 cgtgcgtccg gtcagcaggt cactcatatg cacgtcgata gcatcaaagc ctgcacggtg
    12781 gaaagctgcc gccatttcaa catgcgagtt cacgccctgc tcacgcagta cggcaacttt
    12841 cggacgtgcg ccagtggcaa tatacggtgc tgccacatct tcgttgatat cgaacgacag
    12901 ttttacattc aggcccggat cggcgtcgtt agatttcgcc tgatgctcct gatcggcaca
    12961 ctccgggttg tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc agacacgcaa
    13021 cgtggtgcgg ctttcgctga atacagtctg cccgttggcg gtaatcacaa aacggtcacc
    13081 ggaaaccgcc tgccctacat aatggacaca atcagcaagc ccatgctgtg ccagtacgga
    13141 ctcgaccgct tcacggtcag cggcacgaac ctgaatcacc gcacccagtt cttcgttaaa
    13201 caacgccgcc aggcgatcgt cgcccagaga ggcaatatcc gcgttaatgc cgcaatgacc
    13261 agcaaaggcc atttccgcca gcgttaccag caggccgcca tcagagcggt cgtggtatgc
    13321 gagcagctta cgctgtgcaa ccagcgcctg aatcgcgtca tagaagcctt tcagttgcgc
    13381 gacgtcgcgt acatctgccg gtttgtcgcc aagctgacga taaacctgcg ccagcgccgt
    13441 tgcgcccagc gcgttattgc ctttgcccaa atcaatcagc aatagtgcgt tatcttcggt
    13501 agaaagctgt ggcgtgatgg tgtgacgcac atcttccacg cgggcaaatg cagaaatcac
    13561 cagcgacagc ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc gggttttcat
    13621 cgacatagag tctttaccca ccgggatcgt caggcccagc gccggacaaa gctcttcgcc
    13681 cacggcttta acggcttcat acaggcccgc atcttcacca gggtggcctg ccgccgccat
    13741 ccagttggcg gaaagtttaa tgcgtttgat atcgccaatc tgtgttgcgg caatattggt
    13801 taacgcttca ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg caaccggcgc
    13861 acgctcgcca atcgccatcg cttcaccgta gtagctgtcg aggctggcgg tagtgaccgc
    13921 gcagttagcg accggcacct gccacggacc caccatctga tcgcgcgcta ccatgccagt
    13981 tacgctgcgg tcaccgatgg tcaccaggaa agttttctcc gccacggtcg gcagatgcag
    14041 tatgcgtttc accgcgtctg caatggtgat cccttcacgg accagcgcat cgcccttcgc
    14101 tttcagcgtt tgtacatcgc gggtcatctt cggcgtttta ccaagcagga cgtccagcgg
    14161 cagatcgatc ggctgattat caaaatgacg atcgtgtaga gaaagatgca gttcttcggt
    14221 cgcttcacca atcaccgcgt agggtgcgcg ctcacgctta cacagttcgt caaacagcgg
    14281 taattgatcg gcagcaaccg ccagcacgta gcgttcctgg gattcgttac accagatttc
    14341 cagcgggctc atgcctggct cgtcgctcag aatgtcgcgc agttcaaatt taccgccacg
    14401 cccgccgtcg ctcaccagtt ccggcatagc gttagaaaga ccgcctgcgc caacgtcgtg
    14461 gataaacagt attgggttgg catcaccaag ctgccagcaa cggtcaatca cttcctggca
    14521 gcgacgctcc atctccgggt tgtcgcgctg tacggaagca aagtcgaggt cggcatcaga
    14581 ctgaccagac gccatggaag acgccgcgcc gccgccaaga ccgatgttca ttgccgggcc
    14641 gccgagaacg accagcttcg cgccaacgtt gatctcgcct ttttgtacgt gatcggcgcg
    14701 aatgttgccg atcccgcccg ccagcatgat cggtttgtga taaccgcgca gctcttcgcc
    14761 gttgtggctg ttcacttttt cttcataagt acggaagtag ccgttcagtg ccggacgacc
    14821 aaattcgttg ttaaacgccg caccgcccag cgggccttcg gtcatgatgt ccagcgcggt
    14881 gacaatgcgc tcaggcttac cgaaatcttc ttcccacggc tgttcgaagc caagaattcg
    14941 caggttggaa acggagaaac caaccaaacc cgctttcggc ttcgcgccgc gcccggtggc
    15001 accttcatcg cggatttcac cgccggaacc ggtcgccgcg cccggccacg gagaaatcgc
    15061 cgtcgggtgg ttgtgagttt cgactttcat cagaatatgc gccggttcct gatggaaatc
    15121 atagcggccc gtttcgtggt cagcaaagta gcggcccact tcagaacctt ccattacggc
    15181 ggcgttatct ttataagcag agagaacgtg atctggcgtg gtttcgaaag tatttttgat
    15241 catcctgaac agcgatttcg gctgttgttc accgtcgata atccagtcgg cgttaaaaat
    15301 tttgtggcgg cagtgctcag agttcgcctg agcgaacata tacagctcga tgtcgttcgg
    15361 gttacgacca agctttgtga aagcatcctg cagatagtca atttcatctt ccgccagagc
    15421 caggccaaga cgcaggttag cgtcgatcag cgcctgacgg ccctgcccca gcaaatcaac
    15481 gcttgtgacc ggagtcggtt gatggtgggc aaacaactgc tctgcatcat ctaaatcaaa
    15541 aaagacagtt tccatcatgc ggtcgtgcag ttcagcggta acctgctgcc attgttcatt
    15601 ggtcagcgta ccggcttcta tatagtaagc aacgccgcgc tcaaggcggt ttacctgttg
    15661 tagcccgcag ttatgggcaa tatcggtcgc tttcgaagac cagggagaga tggtgccagg
    15721 acgcggggtc accagcagga gtttgccttg cggggcgtgg ctggcgagtg ccggaccata
    15781 tttcagcagg cgttcaagtt gtgcgtgctc atcatcgttt aacggcgcat tgaggtcagc
    15841 aaaatggaca tactcggcgt aaatattgtg aaccgggagc ctggcagcct gaaaacgtgc
    15901 cagcagtttg ttgattcgga atgccgacag tgcaggcgaa ccacgcagaa tttccatcat
    15961 aagtctctcg tcttctaagc tttcggtgta cccaaggggg ggggaaacgg gcgtcattat
    16021 aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta
    16081 gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg
    16141 ccgctcattt atgagtaaac ctttcactat tattacgttt tttcaagctg ggacgcgcac
    16201 gacacagaga attaactaat tgaaaaaatt aaagattaat tatctgttca tcggcattct
    16261 ggcactgctg ctcgcggtcg ctctctggcc atccattccc tggtttggta aagccgacaa
    16321 ccgtatcgcc gccattcaag cgcggggaga gttgcgtgtg agcaccattc atactcccct
    16381 gacttataac gaaatcaacg ggaaaccttt tggcctggat tacgaactgg cgaaacagtt
    16441 tgccgattac ctcggcgtaa aactgaaagt gaccgtgcgg cagaatatca gccagctgtt
    16501 tgacgacctt gataatggta acgccgacct gctggcggca ggactggtct ataacagtga
    16561 gcgggtaaaa aattatcagc ctggccctac ctattattcc gtgtcacaac aactggttta
    16621 taaagtgggt cagtatcgcc cacgtacgct gtgcaacctg acggcggagc aactcaccgt
    16681 tgcaccgggt catgtggtgg ttaacgatct ccagaccctg aaagaaacaa aattcccgga
    16741 attaagctgg aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga
    16801 aggaaaactc gattacacca ttgctgattc tgtcgccatc agcctgtttc agcgcgttca
    16861 cccggagctc gccgtagcgc tcgatatcac cgatgaacaa ccggtgactt ggtttagccc
    16921 gttagatggc gataataccc tttccgccgc cctgctcgac ttcttcaacg aaatgaatga
    16981 agacggtacg ctggcacgca ttgaagagaa atacctgggg catggcgatg attttgatta
    17041 cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt taaagcccct
    17101 gtttgagaaa tacgccgaag aaattgactg gcgtttgctg gccgctattg cttatcagga
    17161 atcgcactgg gatgcacagg ccacttcacc gacgggtgtg cgcggcatga tgatgttaac
    17221 caaaaatacc gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac agagcatcag
    17281 cggtggcgtg cgttatttgc aggatatgat gagtaaagtg ccggaaagtg tgccggagaa
    17341 cgagcggatc tggtttgccc tcgctgcgta caatatgggc tatgcgcata tgctggatgc
    17401 ccgcgccctg acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg taaaacagcg
    17461 tctgccttta cttagccaga aaccctatta cagcaagctg acttacggct atgctcgtgg
    17521 acatgaagcc tacgcttatg tcgaaaatat tcgtaagtat caaattagcc tggtgggtta
    17581 tctgcaagag aaagagaagc aggctacaga agcggcgatg caactggcgc aggattatcc
    17641 ggcggtatcg cctacggagt tgggcaaaga gaaatttcct tttctctcgt ttctttccca
    17701 gtcgtcatca aactatttga cccattctcc ctctctgctg ttttccagga aagggagtga
    17761 agagaaacaa aattaatccg tcgaggattg cgcttttttc tgcgctttaa tttcctggcg
    17821 gcgcatgcga aagaagtcac tgagcaacgc cgcgcactca tccgccagta ttccttccgt
    17881 aatttccact cggtgattca tacccggatg atgcagcaca tccattaaag atcccgcagc
    17941 gccagttttc gcgtcacgcg caccaaagac cacgcgacca atgcgactgt ggatcatcgc
    18001 tccggcacac attacacatg gttcaagcgt gacatacaac gtggcgtcga tcagacgata
    18061 attttgcatc accagaccac cctgccgcag ggccatgatt tctgcatgtg cggtgggatc
    18121 atggcgacca atcgggcggt tccaaccttc gccgattacc cgattgttat gcactaatac
    18181 cgcgccgacc ggcacttccc gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg
    18241 catccagtat tcgtggctaa attcgacttc agacaaaaag aaaactccgg ttataaaagc
    18301 gcggcgcatt atacacggac gctatgcttt actctattcc agttgctgga gttcaccgcg
    18361 cggggttact cgccaacgat gctgacagaa ataaagcagc ggattgtcct gattactgtc
    18421 gctatagcca ctgtacagcc gcagcggagt gccgattttg cgctccagtt gtgcgacctt
    18481 ttcatgtccc agacaacgca tcgtcaatac ccaaccacca tagccacgct gaatttggct
    18541 ggcgataaga ttaacccgcg gcagccaggg cttatcgaaa taaaccgctt caaccagcgg
    18601 ctgcggagag ccggtaatca accagatatc agcatcggaa cttaacaggt aggtggttaa
    18661 tcgctcctga accagcggaa aggcggtaac attgtcgcga aaccagcgca cgaaatcggc
    18721 ctgcaacgtc tgtaaacgtg cttcgctgtg accaaaagtg cacccccaca gaagcagact
    18781 catcggccag cgtgccgcac gaccttttat caataacgca atggctataa tcggtaacaa
    18841 cggcaggaca agtaacgcat tcagcggttg gcgacgtagt aaatagcgca gaaaactgcc
    18901 gaacatatcc tgctgatgca atgttccatc taagtcaaaa aacaccacac gacgctcgtg
    18961 agttgccaaa ccatactcct ctggactgaa acatcctgat taattttcgc tactacatag
    19021 cctaacagat agatcatcac ttttccggca acaatcgacc gcatcatggc taactggaat
    19081 ttttaattca tggcaattag cggcaatgga atataaaatt cactcgcgtg tgtctcatat
    19141 tgcctgttgt cgccactatt tcatcgcgag gaaagatatg aacggcttac ttcgtatccg
    19201 tcagcgttac caggggcttg cccagagtga taaaaaactg gcggattatc tgctgctaca
    19261 acctgatacg gcgcgccatt taagctctca gcaactggcc aacgaagccg gagtcagtca
    19321 gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa ggttttccgg cgcttaagtt
    19381 ggcgttgagt gaagcgctgg caagccagcc ggaatcgccc tccgtgccca ttcataacca
    19441 aatccgcggt gatgatccgt tacggctggt cggcgaaaaa ctgattaaag aaaatactgc
    19501 cgctatgtac gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat
    19561 gttgcgctct gcgcggcgga taattctgac cggtattggc gcttcgggtc tggtggcgca
    19621 aaactttgcc tggaagctga tgaagattgg cttcaatgct gccgcagtgc gcgatatgca
    19681 tgcgctactc gcaacagtac aggcgtcgtc ccctgacgat ctgttattag ccatttccta
    19741 caccggtgta cgacgcgagt taaacctggc ggcagatgag atgctgcgag tgggcggaaa
    19801 agtgctggcg attaccggct ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct
    19861 gtataccatt gccgaagaac aagcgacaaa cagtgcttca atctctgctt gtcacgctca
    19921 gggaatgtta acggatttgc tgttcattgc gctgattcag caggatctgg aactggcacc
    19981 agaacgtatt cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg
    20041 tataatgccc gcccggtttg tgttgttttg agagtttcct tatggcgttg ttaatcacta
    20101 aaaaatgcat caattgtgat atgtgtgaac ccgaatgccc gaatgaggcg atttcaatgg
    20161 gagatcatat ctacgagatt aacagcgata agtgtaccga atgcgtaggg cactacgaga
    20221 caccaacctg ccagaaggtg tgcccgatcc ccaatactat tgtgaaagat ccggcgcatg
    20281 tcgagacaga agaacagttg tgggataaat ttgtgctgat gcaccacgcg gataaaattt
    20341 aatattctac tctggaagta gagtattaat tatattactg ggaagcctta acgccattat
    20401 atttatttaa ttgatgacat tagcataatc attcactaag ttaatttata tagtatctgc
    20461 caagacactt atttatagtt attaaaggcg cgtccgattg gttcaccgga cgcaccttaa
    20521 gtacgtttcc ttgtgttata agaacagaag gatcagctgt aaaacagcaa tgatgatttt
    20581 gatgacccgt ttaatcaggt atcggcaatc agtcattcgt ttttccttaa acaaggaatg
    20641 cagccgttca gattagccct tacatctccc caaaactgaa cgtgcgagtt attgagggtg
    20701 catgctgcac tccacaccag agctttgacg acaccactcg tttcaatggg ggaattctgt
    20761 ggcatggtgt aaagcacagc aaaatcttca ataacgaagc caattttaat gtacttacga
    20821 attggcggtc acgttaatct ttccatcaat attgctttct tcgtaaaggc tcgagttttt
    20881 atgctaaaga ttgcaagttg cttgtaaaag ataagtacac tgatccataa tcgctgttgt
    20941 tgagggtgca tgctgcacaa aattaaagtt aaaaagtaaa acccccgttc cttaccagtt
    21001 cgggggtttt actttttaaa gagaacggta ttattttaac tttcaataat taccgtggca
    21061 caagcatagt ggcgttcatc tgccagcgtt acatgcatat ttacaacgcc gagcttttcc
    21121 gccagtttta atgcctcacc ccatagccgt agccgtggtt tgccgagctc atcattgaat
    21181 acttcaaatt gattaaacgc cagaccattg cggatcccgg tgccaaacgc ttttgctgcg
    21241 gcttctttca cagcaaaacg cttcgccaga aaacgcaccg gctggtggtg cgttttccag
    21301 attgcccatt cgttatcgct taatacgcgg cgtgctaggc gctcaccgga tcgggcgatc
    21361 actgcttcta tgcgagcgat ctctacgata tccgtgccta aacctaatat tgccattagc
    21421 cacgcgcttc cagcatcaga cgcttcattt ctgccaccgc atctttcagt ccggtcatca
    21481 ctgcacggcc aataatggca tgaccgatat tcagttcatg catctcaggg atggcggcaa
    21541 tggctttcac gttgtgatag gtcagaccgt gtccggcgtt aactttcaga ccgagactag
    21601 cggcaaaggt cgcggctttg gcgatacgca ccagctcttg cgcctgttcg gcgtcagttt
    21661 tggcatcagc atagcaaccg gtgtggatct cgataaacgg tgcgccaacc tctgccgcag
    21721 ctttgatctg ctcttcatcg gcgtcgataa agagagaaac ctgaatcccg gcatctgtca
    21781 gacgtttgca ggcatcgcgc attttttcac gctgccctgc aacgtccagg ccgccttcgg
    21841 ttgttacttc ctgacgcttt tccggtacca ggcagcaaaa atgtggcttc gtctcaacgg
    21901 cgatcgccag catctcttcg gtcactgcca tctccagatt cattcgggta tccagcgtct
    21961 gccgcaggat gcgcacatcg cggtcagtaa tgtggcggcg atcttcacgt aaatgcacgg
    22021 taatgccgtc cgctcccgcc tgctcggcaa taaacgccgc ctgcaccgga tccgggtaag
    22081 cagtaccgcg tgcgttacgc agcgttgcaa tatggtcaat gttgacgcct aacagtaatt
    22141 cagccatgac aatcctcatc attcataatg tgttttcacc gttcgcttag gcataaactg
    22201 ccggaacagt tccctgctct ttaatggttt accgccaaga tacggcttaa gcgccatgcg
    22261 ggtaaagcgt ttcgcggcgc gtagtgtgtc tgcgtcagga aattcccgcg cgtttaacgc
    22321 ttttaactgc cttccggtga acgttttatt gtcgataacg acgcttgcga taaacccttt
    22381 ttcttcgcga taacgatacg tcatggtgtc atctaccggc tcgccgctac ccgcgcaata
    22441 ggtaaaattg acgccatagc ccagatgccc gagcaatgcc agttcaaagc ggcgcagcgc
    22501 gggttctggc gtaccagtgg cacctgcaag agactgaatg cagtgcaaat aatcgaaaaa
    22561 gagttcagag aagcgcgtct cgtattccag tacgcgggag agaagttcgt tgatgtacag
    22621 accgctgtaa agcgtgatac cgcttaatgg cagcgccagc gagacagctt cagcactgcg
    22681 cagcgttttg acttcgccac gcccgccgaa acgtagcaag agaggggtga aaggctgtaa
    22741 tgcacctttc agagtagagc gtttagagcg tgcgcctttg gcaaccagac gcacgcgacc
    22801 cgattcctcc gtgaagacgt ccagcatcag gctggtttcg ctccacgggc gactatgcag
    22861 gacaaatgcg cgctgccagc cttccatcgg agtgactctt aaagatcgtc aacgtaaccg
    22921 agactgcgca gtgcgcgttc gtcgtcggcc caaccggatt tcacttttac ccacagttca
    22981 aggtggacag gcgcttcgaa catttcctgc atgtctttac gcgcttcaat cccgatggtt
    23041 ttgatcttgg cccctttgtt gccaataacc atcttcttct gcccttcacg ctcaacgaga
    23101 atcaaaccat tgatgtcata accaccgcgt tcgttagaga cgaaacgttc gatctccacg
    23161 gtcacggagt acggcagttc agcgccgagg aaacgcatca gtttttcgcg gatgatttca
    23221 gacgccataa aacgctgtga gcgatcggtg atgtaatctt ccgggaagtg atgggtcgct
    23281 tcaggcagat gcttacgcac gattgctgcg atagtgtcga cattcagccc ggtttcagca
    23341 gagatcggca cgatatcgag gaagttcatc tgacttgcca ggaactgtaa gtgcggcagc
    23401 agatcggctt tttcctgcac gttatccact ttgttcaccg cgaggatcac cggcgctttg
    23461 ccatcgcgca gtttgttgag caccatttcg tcgtccggcg tccagcgggt gccttcaacg
    23521 acaaaaatca ccagctcaac gtcgccaata gagctgctcg ccgctttgtt catcaggcgg
    23581 ttaatagcgc gtttttcttc catatgcagg ccaggggtat caacgtagat cgcctgatac
    23641 gcgccttcag tatggatccc cacaatgcgg tgacgagttg tttgcgcttt gcgggaggtg
    23701 atggagatct tctgccccag cagtttgttc aacaatgtgg atttgccaac gttcggacgt
    23761 ccgacgatgg caataaatcc gcagtaactt ttatcgatgc tcattccagc tccagttttt
    23821 tcaacgcctg ttcggcggca gcctgctcag ccttacgacg gcttgaacct gtgccaacca
    23881 ccggttcact caggccgctg acctggcagt ggatagtaaa ttcctgatcg tgcgcttcgc
    23941 cacgtacctg gactaccaga taagtcggca gcggcagatg gcgaccctgc aaatattctt
    24001 gcaagcgcgt tttcggatct ttttgtttat cgcctgggct aatttcgtcc aaacgagttt
    24061 gataccagtt gaggattaat ttctcgacgg tttgaatatc actgtcgagg aatacgccac
    24121 caattaatgc ttcgacggta tcggcgagaa ttgactcacg acgaaatcca ccgcttttaa
    24181 gttcaccagg ccctaaacgt aagcactcgc ctaactcaaa ttcgcgcgcc agttccgcca
    24241 gcgtattgcc acggaccagc gtggcgcgca tccggctcat atcgccttca tccacacgag
    24301 ggaaacggtg ataaagcgca ttggcgataa cgtagctcag aatagagtcg cctaaaaatt
    24361 ctaaacgctc gttatgtttg ctgctggcac tacgatgagt taatgcctgc tgcaacagtt
    24421 cctgatgatt aaaagtgtag cccagcttcc gttgaagccg attaattacg atggggttca
    24481 tgcgatacca ataaataaat gcgtcaacaa ttcagcacac gaaacagacc tgatatacat
    24541 ggctctgcta actgcttcgc tgcagtttca gtttaccggt atatggggac caacgctgtt
    24601 tcgtgtgccg tggcaacctg gaggtgccaa ccttaaactt cgggggaata ttctatacac
    24661 aacgacgggg gatgtcgtta gccacgggag atttatctca taaataattc acgttgtcgc
    24721 cataacggcg acaacgtgaa cgaagatggg ctattaatgg atgccgccaa tgcgacttaa
    24781 gcgcacacct gtcggccatt cgccttcttg cttatcgaaa ctcatccaga tagccgttgc
    24841 ccgaccgacc agattcgctt caggcacaaa accccagtaa cggctgtccg cgctgttgtc
    24901 gcggttgtcg cccatcatga agtattgtcc cggcggaaca atccaggttg ccagttgttg
    24961 ccctggctgc tggtaataca tccccacctg gtcctgcgca atcggcactg tcagaattcg
    25021 gtgcgtcaca tcacccagtg tctctttacg ctcggaaaga cgaattccat tttctttggt
    25081 ttcgtttttc ggcacttcaa agaatccgct ggtcgcttcc ccaccattac ggcgtgagaa
    25141 ggtctgaacg aaatcgctcg gttccacgtt ggagtaggtg accggtagcg cgttttcaca
    25201 cgcctggccg gaactgcatc ccggttgaat cgtcagctct tttgagaccg gatcgtaagt
    25261 gactttatcg cccggtaaac ccaccgcgcg cttgatgtaa tcaagctttg gatcttccgg
    25321 atatttaaag accacgatat cgccgcgttt cggatgaccg gtttcgatca gcgttttctg
    25381 gtagatagga tctttaatgc cataagcaaa cttctctacc agaataaaat caccgattaa
    25441 cagagtcggc atcatcgaac ctgacgggat ctggaacggt tcataaataa acgaacgtac
    25501 aatcaatacg atagccagca ccggaaaaac agaagcaccg gtttccagcc agccaggctt
    25561 cggcgcaacc tttttcaggg ttgctttatc cagtgagtca ccggcagccg cctgcgccgc
    25621 tgcctgacgt tcccgccgtt taggtgcgaa aaagaattta tccacgcacc ataaaatgcc
    25681 cgtcaccagt gtggcaatca ccagaatcag ggcaaacata ttcgccatgc caactcctaa
    25741 gggttatttg ttgtctttgc cgacgtgcag aatggcgagg aacgcttcct gcggcagctc
    25801 gacgttaccg atctgcttca tgcgtttctt accttctttc tgcttctgca gcagcttttt
    25861 cttacggctg atatcgccgc cataacattt agccagtacg tttttacgca gctgtttcac
    25921 ggtggatcgc gcaataatgt gcgtaccaat cgctgcctga atggcgatat caaactgctg
    25981 gcgtgggatc agatctttca tcttctccac caactcgcga ccgcggtttt gcgaattatc
    26041 acggtgggtg atcagcgcca acgcatcaac acgttcaccg ttgattaata cgtctacacg
    26101 taccatgtcg gacgcctgga agcgtttgaa gttgtaatcc agagacgcat aaccacgcga
    26161 ggtagatttc aggcgatcga agaagtcgag caccacttcc gccatcggga tctcgtacgt
    26221 cagcgccacc tgattaccgt ggtaaaccat attggtctgc acgccgcgtt tttctacgca
    26281 cagcgtaata acgttgccga gatatgcctg cggcagcagc atgtgacact ctgcaatcgg
    26341 ctcgcgcagt tcgtaaatgt tatttaccgc aggcagcttg gatgggctgt cgacgtagat
    26401 aacttccctt gacgtggttt caacttcata cactacggtc ggcgcagtgg tgatcagatc
    26461 cagatcgtat tcacgttcca gacgttcctg gatgatctcc atgtgcagca ggccaaggaa
    26521 gccgcagcgg aaaccaaagc ccagcgcgct ggagctttcc ggctcataga acagtgaggc
    26581 atcattcagg ctgagtttac ccagcgcgtc acggaaggct tcatagtcgt cggaacttac
    26641 cgggaacaga ccggcgtata cctgcggttt gactttctta aagccaggca gcgccttttc
    26701 tgccggatta cgcgccagcg ttaaggtatc gccgactgga gcgccgtgga tatctttaat
    26761 cgcacatacc agccagccta cttcgccaca tttcagttca gtgcggtcaa cctgtttcgg
    26821 cgtgaagatg cccagacggt cggcgttata ggtctgcccg gtactcatga ctttcacttt
    26881 gtcgccctta cgcagggtgc cgtttttaat acggataagt gaaacaacgc ccaggtagtt
    26941 gtcgaaccat gagtcgataa ttagtgcctg caacgggcct tccggatcgc cttccggcgg
    27001 cggaatgtcg cgcaccagac gttcgagaac gtcctgcacg ccaacgccgg ttttcgctga
    27061 acagcgcacc gcgtcagtgg cgtcgatgcc gacgatatct tcaatttctt ccgccacgcg
    27121 ttcaggatcg gctgccggca ggtcaatctt gttcagtact ggcaccactt cgagatccat
    27181 ttccatggcg gtgtagcagt ttgccagggt ttgcgcttcc acgccctgcc cggcgtcgac
    27241 caccagcaat gcaccttcac aggcagccag cgaacgggaa acttcatagg agaagtctac
    27301 gtggcccggg gtgtcgataa agttaagctg ataggtttcg ccgtcagacg ctttgtagtc
    27361 cagcgtcacg ctttgcgctt tgatggtaat gccacgctca cgctcaagat ccatggaatc
    27421 gagaacctgc gcctccattt cacggtcaga caggccaccg cagatctgga taatacggtc
    27481 agacagcgtc gatttaccgt ggtcaatgtg agctatgatc gaaaagttac gtatattctt
    27541 cataaagtat gattattgtg ccttaatgcc cggttaacca ggcttttaga agtcgctgtt
    27601 ctgagcttaa cgtctgtatt aatagaaacg ccgcattcta cactacaaca ttgaggcgag
    27661 gaaatgttca taccgtatgg attgtggtat ctggaaacgt cctcgcattt gttatgcaaa
    27721 atgcaacaaa gccagtgaaa tcactggctc gcgtcttccg aagatgtttc aaatcgcaca
    27781 aggccaggcg gcaatgccac gcttaagatg atcggttgcc attctgcccg ggcagcaaac
    27841 ttacgcgagt agccgcgcgc aatcaggaac ccgccaatac cgccgaggac cgcaccacat
    27901 aatgctgcga cgtcagaagc aaagagtagc tgaaatagcg aagcgatgag gaataatccc
    27961 accagcggcg acatataaac cagtaatgcg gagctaagca ggctgccttc ggcgatcccc
    28021 aattccactt tttgccccgg cactaacggt tcatcacagg gtacgacaat ggtatgcgtg
    28081 gtttgcgggc caagtttatt caacacgcgg ctaccgcaac cggcgcgtga agcgcagctg
    28141 ctgcatgaag ctttaacatc acaactgacc agcgcctgcc cgttttgcca ggagacgacg
    28201 gtagcccact ctttgatcat tgcgctgccc cgaacttaat attctcggca atgcgtttcg
    28261 ccgtttgcgg cggcagttca ccgacaatgg tgatttcggc gttatcacgt acgcttgtac
    28321 tgacggttct gcgtccggtg cgcaacatct gatcggtgct cgatggcgta gcgcggttaa
    28381 cgtttaccga gaagctgaat aatccgtcag aatagagacg tgattcgata ggcatgttgt
    28441 ccatcgtcgg tagcggacgt cgactactgg aaacttcgct aaaaccctgt ggcaaccagg
    28501 ttggcgtcca gctgaattta gctttttcac ctacaggaac agaaagcaac ggcggcaaat
    28561 ttgccttcgc cagcgtctgc atactgctgc tgatatcctg attgacgtta aaagcaatca
    28621 cgcgaaattg ttccagcgtt tcaccatcgc gatcaaggag atcaacccgc atcggtaatt
    28681 tcgattcggt gtccatccac acgatgtagc tgtagcgtgt accatctcgg gcaaccacgc
    28741 gaatgacttc gcaaagacga tcagcaatac gcgtgcgccc gacggagata aagtcgtagt
    28801 aaggagaaag gcgtttgaaa tcggtataga tgagcgatgg cagagaatca acaatgtaat
    28861 cgccattaag cgtgaacggt tcaagtcccg gttcaaaata gctgatttca tcgccgcgct
    28921 gtaccacttc ccggcgcggg ccatccattt gcaacaattg tgcaagagga cggttatcga
    28981 ggcgtgcatg tcgataacgc agagactcaa caccctgttt attgatgctg atgaatgaca
    29041 gctcgtaatt cagtgactga ctggccaggt tcatctgctg taataacgcc ccggacgcgg
    29101 gagtggccga ggcgttagca gagaataaca ggctacctgt cactaatgac atggcaaacc
    29161 aaagttgctt cattactgcg attgcgttcc taaagtttga attcctggca cctgtacagc
    29221 ggcttgctgg gtttgcgcct gctcaaactg aagctgttca gagtggagtc ggcgttgcag
    29281 ttcgtaatcc tgcaacattg cattaatgcg acgacgctgc tcctgtacct gctgctgttg
    29341 accattgttt gcggtcgctt cagaaggtac tcccaggctt accgggctgg ctttacccat
    29401 catcggcagt gtattaaata ccggcgtttc gggctgctgg gacgtttcag attgtccatt
    29461 atagtgctgg acgccaacga taactgcaag cgatacgcat gctgctacgc ccatttgggt
    29521 aagctgtgcc gcccacggac gtactttctg ccagaatggc attttctgcc attgatgcgg
    29581 cgcaggctgg gcttccggga tcaatgtcgc cggttgacgt actggctctt cttcaatggc
    29641 ggccatcacg cgtgaagaga tatcgaaatg gagcacctcg ggagtatcac cccgcattga
    29701 gtcacggatt aagtgatagc tttcccaggt tttctgcatt tctgggttat gagccagttc
    29761 gttaagcagc tcactatcca gcgtttcgcc atccattaaa gcggaaagtt gttctttctg
    29821 catgcctaat acccttatcc agtatcccgc tatcgtcaac gcctgataag cggttgaact
    29881 ttgttatcaa tagcttccct cgctcggaag atacgtgaac gcaccgtacc taccggacaa
    29941 tccatgatag cggctatctc ttcatagctc aggccatcca gctcccgcaa ggttattgcc
    30001 atgcgcaaat cttccgggag ggactcaata gttcggaaaa ctatctgtct cagttcttct
    30061 gacaacatta agttctcagg gttcgaaatt tctttcaacg cgccgccact ttcgaagttt
    30121 tcagcttcaa tggcatccac atcactggaa ggtggacgac gcccctgagc aaccaggtaa
    30181 tttttcgctg tatttacagc aatccgatac agccatgtat aaaaagcgct atctccccgg
    30241 aacgaatcca gcgcacgata ggctttaata aaagcttctt gtaccacatc gggaacatca
    30301 cccgacggca catagcggga aaccagactc gccactttat gctgatagcg cactaccagt
    30361 aagttaaagg ctttctgatc tcccttctgg acccgttcaa ccaggacctg gtccgttaac
    30421 tgctcgctca tccgaggtaa agtctcccca aaccaaattt ccacgcgcta tcgaaacgcc
    30481 actccattag ctgcaatttg agcaagcaaa gggttagagt gtctcgtttt tgtaaagttc
    30541 cgtaacgcat ctgtttttgt ttgtcatgct gtagacggat cattatctat cattataagt
    30601 ctacagaatc tgaacatcgc attatctgtg tagaaatgcc catttaactg cctgaagagt
    30661 aacccaacgg cctttttatt tcaccaccta atcctccacc agccagtaac ttctcttttt
    30721 ctcgccgccc tgcatcagcg tgtttagcaa ctgtaacaaa tattaaaata gcaggtgttt
    30781 attcgcacaa catgatgcta tactgaccaa accgtgttta gtaaattaaa caaagaaaat
    30841 gaatactctc cctgaacatt catgtgacgt gttgattatt ggtagcggcg cagccggact
    30901 ttcactggcg ctacgcctgg ctgaccagca tcaggtcatc gttctaagta aaggcccggt
    30961 aacggaaggt tcaacatttt atgcccaggg cggtattgcc gccgtgtttg atgaaactga
    31021 cagcattgac tcgcatgtgg aagacacatt gattgccggg gctggtattt gcgatcgcca
    31081 tgcagttgaa tttgtcgcca gcaatgctcg ttcctgtgtg caatggttaa ttgaccaggg
    31141 ggttttgttt gatacccacg ttcaaccgaa tggcgaagaa agctaccatc tgacccgtga
    31201 aggaggacat agtcaccgtc gtattcttca tgccgccgac gccaccggta gagaagtaca
    31261 atccacgctg gtgagcaagg cgcagaacca tccgaatatt cgtgtgctgg agcgcagcaa
    31321 cgcagttgat ctgattgtct ctgacaaaat tggcctgccg ggcacgcgac gg
//
LOCUS       DAJTOR010000030        30944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTOR010000030 DAJTOR010000000
VERSION     DAJTOR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.030"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO249_001217"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO249_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5635587.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO249_001218"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO249_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5635588.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1338..1766
                     /gene="rplK"
                     /locus_tag="OO249_001219"
     CDS             1338..1766
                     /gene="rplK"
                     /locus_tag="OO249_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5635589.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGAGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1770..2474
                     /gene="rplA"
                     /locus_tag="OO249_001220"
     CDS             1770..2474
                     /gene="rplA"
                     /locus_tag="OO249_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5635590.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2887..3384
                     /gene="rplJ"
                     /locus_tag="OO249_001221"
     CDS             2887..3384
                     /gene="rplJ"
                     /locus_tag="OO249_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5635591.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3451..3816
                     /gene="rplL"
                     /locus_tag="OO249_001222"
     CDS             3451..3816
                     /gene="rplL"
                     /locus_tag="OO249_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5635592.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4136..8164
                     /gene="rpoB"
                     /locus_tag="OO249_001223"
     CDS             4136..8164
                     /gene="rpoB"
                     /locus_tag="OO249_001223"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5635593.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8241..12464
                     /gene="rpoC"
                     /locus_tag="OO249_001224"
     CDS             8241..12464
                     /gene="rpoC"
                     /locus_tag="OO249_001224"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5635594.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12677..13216
                     /locus_tag="OO249_001225"
     CDS             12677..13216
                     /locus_tag="OO249_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HCR5635595.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="OO249_001226"
     CDS             complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="OO249_001226"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5635596.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAKTEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="OO249_001227"
     CDS             complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="OO249_001227"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5635597.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="OO249_001228"
     CDS             complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="OO249_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5635598.1"
                     /translation="MQILFNDQPMQCATGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="OO249_001229"
                     /pseudo
     CDS             complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="OO249_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="OO249_001230"
     CDS             complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="OO249_001230"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5635599.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVISAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="OO249_001231"
     CDS             complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="OO249_001231"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5635600.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19071..19171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="OO249_001232"
     CDS             complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="OO249_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5635601.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQKVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19795..20568
                     /gene="nudC"
                     /locus_tag="OO249_001233"
     CDS             19795..20568
                     /gene="nudC"
                     /locus_tag="OO249_001233"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5635602.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20608..21672
                     /gene="hemE"
                     /locus_tag="OO249_001234"
     CDS             20608..21672
                     /gene="hemE"
                     /locus_tag="OO249_001234"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5635603.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKVTRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21682..22353
                     /gene="nfi"
                     /locus_tag="OO249_001235"
     CDS             21682..22353
                     /gene="nfi"
                     /locus_tag="OO249_001235"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5635604.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22396..22986
                     /locus_tag="OO249_001236"
     CDS             22396..22986
                     /locus_tag="OO249_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5635605.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVVLSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23173..23445
                     /gene="hupA"
                     /locus_tag="OO249_001237"
     CDS             23173..23445
                     /gene="hupA"
                     /locus_tag="OO249_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5635606.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23458..24153
                     /locus_tag="OO249_001238"
     CDS             23458..24153
                     /locus_tag="OO249_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5635607.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="OO249_001239"
     CDS             complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="OO249_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5635608.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24818..26194
                     /gene="zraS"
                     /locus_tag="OO249_001240"
     CDS             24818..26194
                     /gene="zraS"
                     /locus_tag="OO249_001240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5635609.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26191..27515
                     /gene="zraR"
                     /locus_tag="OO249_001241"
                     /pseudo
     CDS             26191..27515
                     /gene="zraR"
                     /locus_tag="OO249_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(27512..28801)
                     /gene="purD"
                     /locus_tag="OO249_001242"
     CDS             complement(27512..28801)
                     /gene="purD"
                     /locus_tag="OO249_001242"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5635610.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLLLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28813..30402)
                     /gene="purH"
                     /locus_tag="OO249_001243"
     CDS             complement(28813..30402)
                     /gene="purH"
                     /locus_tag="OO249_001243"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5635611.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7337 a         7988 c         8433 g         7186 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgactcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcttggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgagtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gcgagattgg aatacaattt cgcgcctttt atttttatgg
     1261 gccttgcccg taaaaacgat tttttatatc acggggagcc tctcagaggc gttattaccc
     1321 aacttgagga atttataatg gctaagaaag tacaagccta tgtcaagctg caggttgcag
     1381 ctggtatggc taacccgagt ccgccagtag gtccggctct gggtcagcag ggcgtaaaca
     1441 tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga
     1501 ttccggtagt aatcaccgtt tacgctgacc gttctttcac tttcgttacc aagaccccgc
     1561 cggcagcagt tctgctgaaa aaagcggctg gtatcaagtc tggtgccggt aagccgaaca
     1621 aagacaaagt gggtaaaatt tcccgcgctc agctgcagga aatcgcgcag accaaagctg
     1681 ccgacatgac tggtgccgac attgaagcga tgactcgctc catcgaaggt actgcacgtt
     1741 ccatgggcct ggtagtggag gactaagaaa tggctaaact gaccaagcgc atgcgtgtta
     1801 tccgcgagaa agttgatgca accaaacagt acgacatcaa cgaagctatc gcactgctga
     1861 aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg
     1921 acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt actgccgcac ggtactggcc
     1981 gttccgttcg cgtagccgta tttacccaag gtgcaaacgc tgaagctgct aaagctgcag
     2041 gcgcagaact ggtaggtatg gaagatctgg ctgaccagat caagaaaggc gaaatgaact
     2101 ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc
     2161 tgggtccgcg cggcctgatg ccaaacccga aagtgggtac tgtaacaccg aacgttgctg
     2221 aagcggttaa aaacgctaaa gctggccagg ttcgttaccg taacgacaaa aacggcatca
     2281 tccacaccac catcggtaaa gtggactttg acgctgacaa actgaaagaa aacctggaag
     2341 ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga
     2401 aagttagcat ctccaccacc atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg
     2461 cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt ctacaatctt acccccacgt
     2521 ataatgctta atgcagacgt atatccgaga tattcgggtt gtggcaaggc ggcaactgag
     2581 tgagtcgcca agagcatagc taactatgtg actggtgcga atgaaggaag ccaacgccgc
     2641 cacaagttga atagcgacgg atagaaaaga tttgttcgtt ggagcttggc ctatccaggc
     2701 ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc ccctgcgtag acggtgacag
     2761 aacgctaaga ttattctttt atattctggc ttgtttctgc tcaccgtaat taagacgctc
     2821 tctccgtttg gaggagtgaa gtgagttcca gagattttct ctggcaaaca tccaggagca
     2881 aagctaatgg ctttaaatct tcaagacaaa caagcgattg ttgctgaagt cagcgaagta
     2941 gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg gcgtaactgt agataaaatg
     3001 actgaactgc gtaaagcagg tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc
     3061 ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt
     3121 ccgaccctga ttgcatactc tatggaacac ccgggcgctg ctgctcgtct gttcaaagag
     3181 ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg cctttgaagg tgagctgatc
     3241 ccggcgtctc agatcgaccg cctggcaact ctgccgacct acgaagaagc aattgcacgc
     3301 ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg ttcgtactct ggctgctgta
     3361 cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt aacgcattcg cttacgtata
     3421 aacttattct gatattcagg aacaatttaa atgtctatca ctaaagatca aatcattgaa
     3481 gcagttgcag ctatgtctgt aatggacgtt gtagaactga tctctgcaat ggaagaaaaa
     3541 ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg gcccggttga agctgctgaa
     3601 gaaaaaactg aattcgacgt aattctgaaa gctgctggcg ctaacaaagt tgctgttatc
     3661 aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag ctaaagacct ggtagaatct
     3721 gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg cagaagcact gaaaaaagct
     3781 ctggaagaag ctggcgctga agttgaagtt aaataagcca acccttccgg ttgcagcctg
     3841 agaaatcagg ctgatggctg gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc
     3901 cagtagcgtt tcacactgtt tgactactgc tgtgcctttc aatgcttgtt tctatcgacg
     3961 acttaatata ctgcgacagg acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg
     4021 tgatagcaac aggcattgcg gaaagtgttc cattttccgg tcaacaaaat agtgttgcac
     4081 aaactgtccg ctcaatggac agatgggtcg acttgtcagc gagctgagga accctatggt
     4141 ttactcctat accgagaaaa aacgtattcg taaggatttt ggtaaacgtc cacaagttct
     4201 ggatgtacct tatctccttt ctatccagct tgactcgttt cagaaattta tcgagcaaga
     4261 tcctgaaggg cagtatggtc tggaagcagc tttccgttcc gtattcccga ttcagagcta
     4321 cagcggtaat tccgagctgc aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt
     4381 ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct
     4441 ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa gacattaaag aacaagaagt
     4501 ctacatgggc gaaattccgc tcatgacaga caacggtacc tttgttatca acggtactga
     4561 gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg
     4621 taaaacccac tcttcgggta aagtgctgta taacgcgcgc atcatccctt accgtggttc
     4681 ctggctggac ttcgaattcg atccgaagga caacctgttc gtacgtatcg accgtcgccg
     4741 taaactgcct gcgaccatca ttctgcgcgc cctgaactac accacagagc agatcctcga
     4801 cctgttcttt gaaaaagtta tctttgaaat ccgtgataac aagctgcaga tggaactggt
     4861 gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta
     4921 cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc cagctggaaa aagacgacgt
     4981 caaacttatc gaagtcccgg ttgagtacat cgcaggtaaa gtggttgcta aagactatat
     5041 tgatgagtct accggcgagc tgatctgcgc agcgaacatg gagctgagcc tggatctgct
     5101 ggctaagctg agccagtctg gtcacaagcg tatcgaaacg ctgttcacca acgatctgga
     5161 tcacggccca tatatctctg aaaccttacg tgtcgaccca actaacgacc gtctgagcgc
     5221 actggtagaa atctaccgca tgatgcgccc tggcgagccg ccgactcgtg aagcagctga
     5281 aagcctgttc gagaacctgt tcttctccga agaccgttat gacttgtctg cggttggtcg
     5341 tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa ggttccggta tcctgagcaa
     5401 agacgacatc attgatgtta tgaaaaagct catcgatatc cgtaacggta aaggcgaagt
     5461 cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa
     5521 ccagttccgc gttggcttgg tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg
     5581 cgatctggat accctgatgc ctcaggatat gatcaacgcc aagccgattt ccgcagcagt
     5641 gaaagagttc ttcggttcca gccagctgtc tcagtttatg gaccagaaca acccgctgtc
     5701 tgagattacg cacaaacgtc gtatctccgc actcggccca ggcggtctga cccgtgaacg
     5761 tgcaggcttc gaagttcgag acgtacaccc gactcactac ggtcgcgtat gtccaatcga
     5821 aacccctgaa ggtccgaaca tcggtctgat caactctctg tccgtgtacg cacagactaa
     5881 cgaatacggc ttccttgaga ctccgtatcg taaagtgacc gacggtgttg taactgacga
     5941 aattcactac ctgtctgcta tcgaagaagg caactacgtt atcgcccagg cgaactccaa
     6001 cctggatgaa gaaggccact tcgtagaaga cctggtaact tgccgtagca aaggcgaatc
     6061 cagcttgttc agccgcgacc aggttgacta catggacgta tccacccagc aggtggtatc
     6121 cgtcggtgcg tccctgatcc cgttcctgga acacgatgac gccaaccgtg cattgatggg
     6181 tgcgaacatg caacgtcagg ccgttccgac tctgcgcgct gataagccgc tggttggtac
     6241 tggtatggaa cgtgctgttg ccgttgactc cggtgtaact gcggtagcta aacgtggtgg
     6301 tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa gttaacgaag acgagatgta
     6361 tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa tacacccgtt ctaaccagaa
     6421 cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt
     6481 gctggcagac ggtccgtcca ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt
     6541 agcgttcatg ccgtggaatg gttacaactt cgaagactcc atcctcgtat ccgagcgtgt
     6601 tgttcaggaa gaccgtttca ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga
     6661 caccaagctg gggccggaag agatcaccgc tgacatcccg aacgtgggtg aagctgcgct
     6721 ctccaaacta gatgaatccg gtatcgttta cattggtgcg gaagtgaccg gtggcgacat
     6781 tctggttggt aaggtaacgc cgaaaggtga aactcagctg accccagaag aaaaactgct
     6841 gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac tcttctctgc gcgtaccaaa
     6901 cggtgtatcc ggtacggtta tcgacgttca ggtctttact cgcgatggcg tagaaaaaga
     6961 caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag gcgaagaaag acctgtctga
     7021 agaactgcag atcctcgaag cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg
     7081 tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat cgctggctgg agctgggcct
     7141 gacagacgaa gagaaacaaa atcagctgga acagctggct gagcagtatg acgaactgaa
     7201 acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct
     7261 ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg gttaaacgcc gtatccagcc
     7321 tggtgacaag atggcaggtc gtcacggtaa caagggtgta atttctaaga tcaacccgat
     7381 cgaagatatg ccttacgatg aaaacggtac gccggtagac atcgtactga acccgctggg
     7441 cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc cacctgggta tggctgcgaa
     7501 aggtatcggc gacaagatca acgccatgct gaaacagcag caagaagtcg cgaaactgcg
     7561 cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt cgtcagaaag ttgacctgag
     7621 taccttcagc gatgaagaag ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat
     7681 cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg
     7741 cgacctgccg acttccggtc agatccgcct gtacgatggt cgcactggtg aacagttcga
     7801 gcgtccggta accgttggtt acatgtacat gctgaaactg aaccacctgg tcgacgacaa
     7861 gatgcacgcg cgttccaccg gttcttacag cctggttact cagcagccgc tgggtggtaa
     7921 ggcacagttc ggtggtcagc gtttcgggga gatggaagtg tgggcgctgg aagcatacgg
     7981 cgcagcatac accctgcagg aaatgctcac cgttaagtct gatgacgtga acggtcgtac
     8041 caagatgtat aaaaacatcg tggacggcaa ccatcagatg gagccgggca tgccagaatc
     8101 cttcaacgta ttgttgaaag agattcgttc gctgggtatc aacatcgaac tggaagacga
     8161 gtaattctcg ctcaaacagg tcactgctgt cgggttaaaa cccggcagcg gattgtgcta
     8221 actccgacgg gagcaaatcc gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa
     8281 ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc agacatgatc cgttcatggt
     8341 ctttcggtga agttaaaaag ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg
     8401 acggcctttt ctgcgcccgt atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta
     8461 agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc
     8521 agactaaagt acgccgtgag cgtatgggcc acatcgaact ggcttccccg actgcgcaca
     8581 tctggttcct gaaatcgctg ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg
     8641 atatcgaacg cgtactgtac tttgaatcct atgtggttat cgaaggcggt atgaccaacc
     8701 tggaacgtca gcagatcctg actgaagagc agtatctgga cgcgctggaa gagttcggtg
     8761 acgaattcga cgcgaagatg ggtgcggaag caatccaggc tctgctgaag agcatggatc
     8821 tggagcaaga gtgcgaacag ctgcgcgaag agctgaacga aaccaactcc gaaaccaagc
     8881 gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac
     8941 cagagtggat gatcctgacc gttctgccgg tactgccgcc agatctgcgt ccgctggttc
     9001 cgctggatgg tggtcgtttc gcgacttctg acctgaacga tctgtatcgt cgcgtcatta
     9061 accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc gccggacatc atcgtacgta
     9121 acgaaaaacg tatgctgcag gaagcggtag acgccctgct ggataacggt cgtcgcggtc
     9181 gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta
     9241 aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg
     9301 taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc
     9361 tggagctgtt caaaccgttc atctacggca agctggaact gcgtggtctt gctaccacca
     9421 ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt cgtttgggat atcctggacg
     9481 aagttatccg cgaacacccg gtactgctga accgtgcacc gactctgcac cgtctgggta
     9541 tccaggcatt tgaaccggta ctgatcgaag gtaaagctat ccagctgcac ccgctggttt
     9601 gtgcggcata taacgccgac ttcgatggtg accagatggc tgttcacgta ccgctgacgc
     9661 tggaagccca gctggaagcg cgtgcgctga tgatgtctac caacaacatc ctgtccccgg
     9721 cgaacggcga accaatcatc gttccgtctc aggacgttgt tctgggtctg tactatatga
     9781 cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct gactggcccg aaagaagcag
     9841 aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg
     9901 agtatgaaaa agatgcaaac ggtgaattag tagcgaaaac cagcctgaaa gacacgactg
     9961 ttggccgtgc cattctgtgg atgattgtac cgaaaggtct gccttactcc atcgtcaacc
    10021 aggcgctggg taaaaaagca atctccaaaa tgctgaacac ctgctaccgc attctcggtc
    10081 tgaaaccgac cgttattttt gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc
    10141 gttctggtgc atctgttggc atcgatgaca tggtcatccc ggagaagaaa cacgaaatca
    10201 tctccgaggc agaagcagaa gttgctgaaa ttcaggagca gttccagtct ggtctggtaa
    10261 ctgcgggcga acgctacaac aaagttatcg atatctgggc tgcggcgaac gatcgtgtat
    10321 ccaaagcgat gatggataac ctgcaaactg aaaccgtgat taaccgtgac ggtcaggaag
    10381 agaagcaggt ttccttcaac agcatctaca tgatggccga ctccggtgcg cgtggttctg
    10441 cggcacagat tcgtcagctt gctggtatgc gtggtctgat ggcgaagccg gatggctcca
    10501 tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct gaacgtactc cagtacttca
    10561 tctccaccca cggtgctcgt aaaggtctgg cggataccgc actgaaaact gcaaactccg
    10621 gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct ggtggttacc gaagacgatt
    10681 gtggtaccca tgaaggtatc atgatgactc cggttatcga aggtggtgat gttaaagagc
    10741 cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg
    10801 ctgatatcct cgttccgcgc aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag
    10861 agaactctgt cgacgcggtt aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg
    10921 tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca catcatcaac aagggtgaag
    10981 caatcggtgt tatcgcggca cagtccatcg gtgaaccggg tacacagctg accatgcgta
    11041 cgttccacat cggtggtgcg gcatctcgtg cggctgctga atccagcatc caggtgaaaa
    11101 acaaaggtag catcaagctc agcaacgtga agtcggttgt gaactccagc ggtaaactgg
    11161 ttatcacttc ccgtaatact gaactgaaac tgatcgacga attcggtcgt accaaagaaa
    11221 gctacaaagt accttacggt gcggtactgg cgaaaggcga tggcgaacag gttgctggcg
    11281 gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt
    11341 ttgtacgctt tactgacatg atcgacggtc agaccattac tcgtcagaca gacgaattga
    11401 ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg taccgcaggt ggtaaagatc
    11461 tgcgtccggc actgaaaatc gttgatgctc agggtaacga cgttctgatt ccgggtaccg
    11521 atatgcctgc gcagtacttc ctgccgggta aagcgattgt tcagctggaa gatggcgtac
    11581 agatcagctc tggtgacacc ctggcgcgta ttccgcagga atccggcggt accaaggaca
    11641 tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc acgtcgtccg aaagagccgg
    11701 caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc
    11761 gtcttgttat caccccggta gacggtagcg atccgtacga agagatgatt ccgaaatggc
    11821 gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc
    11881 cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca tgctgttact cgttacatcg
    11941 ttaacgaagt acaggacgta taccgtctgc agggcgttaa gattaacgat aaacacatcg
    12001 aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt taacgcgggc agctccgact
    12061 tcctggaagg cgaacaggtt gaatactctc gcgtcaagat cgcaaaccgc gaactggaag
    12121 cgaacggcaa agtgggtgca acttactccc gcgatctgct gggtatcacc aaagcgtctc
    12181 tggcaaccga gtccttcatc tccgcggcat cgttccagga gaccactcgc gtgctgaccg
    12241 aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg
    12301 gtcgtctgat cccggcaggt accggttacg cgtaccacca ggatcgtatg cgtcgccgtg
    12361 ctgcgggtga agctccggct gcaccgcagg tgactgcaga agacgcatct gccagcctgg
    12421 cagaactgct gaacgcaggt ctgggcggtt ctgataacga gtaatcgtta atccgcaaat
    12481 aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg
    12541 tcagaatatt taaggaattt ctgaatactc ataatcaatg tagagatgac taatatcctg
    12601 aaactgactg aactaattga gtcaaactcg gcaaggattc gatactattc ctgtgtaact
    12661 ttcttaagga acgagaatga aacaggaagt ggaaaagtgg cgaccttttg gacatccgga
    12721 tggtgatatt cgtgatttat catttcttga tgctcatcag gctgtctacg ttcagcatca
    12781 tgagggcaaa gagcctttag agtatcgctt ttgggttacc tactctcttc actgcttcac
    12841 aaaagattat gaacatcaga cgaacgaaga aaaacaatcg ttaatgtacc acgcgcctaa
    12901 agaatctcgt cccttctgcc agcaccgtta taacttagcg cgcacacact taaaaagaac
    12961 tattttggcg ctgccagaaa gcaacgttat tcatgccggg tatggtagct atgccgtgat
    13021 tgaggtggac ttagacggag gagataaggc attttacttt gttgcgttca gggctttcag
    13081 ggaaaagaaa aaactccgtt tgcatgtaac tagcgcttat cccatttctg aaaaacagaa
    13141 aggtaaatca gtgaaatttt tcaccattgc ctacaactta ttgagaaata agcagcttcc
    13201 tcagccctca aaataacaaa acccacctta aggtgggttt cgccagagaa ttatctctgg
    13261 tattcagaac gccattaccg gactttgcct tgaccttgcg ataatcgcag gttgcgggat
    13321 gtctgaattt cttcagtctg ctgcatcctg gaagatgaga acatgtgttc ttattttcgt
    13381 ctctatcata gttgagtatt tactctctta caatcagatc tctttcattg ctcaacaggc
    13441 gatggcttca gactttgcat tacggaattt ttaagaaagg cagggcgaaa cgaggaagaa
    13501 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    13561 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    13621 ccgtctcata gtctttgcga ggcccgtccc agatagctgt cccagtcttt ccataccggc
    13681 tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13741 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13801 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13861 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13921 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13981 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    14041 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    14101 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    14161 tgttttttgc ctttcagatg atggcgggca taagtcgcct cgtgatatgt ctcctgataa
    14221 accatcacgc catccagacc aagctgcttt aactcggcgt attccgtctt cgccagcggt
    14281 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    14341 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    14401 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    14461 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    14521 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    14581 cgttgggcca gttgttccag atagccactg gcggctggcg ataacagcgc catcatatcg
    14641 tcgcgggtga attgcgaggc atttagcgcc cgctctacgt caacagccgt tttgccgttg
    14701 atacgcaggc ggatgtcatc ccagtccagt tgtcgccagc gatcgctgaa ggttttcatg
    14761 ccgatgcctc cagaaatcca gtcagcgggc tggtggcatg agcaaaatga ctgcggctgc
    14821 ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc cagacgaaat gccttcgcca
    14881 tgttgacggg atcgtccgct acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14941 tttccagggc ctgcgctgca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    15001 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    15061 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    15121 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcatttcgg
    15181 cggctttcag agtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcta
    15241 attttaacca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    15301 ccgctgtttt cgccccggaa gtatttggca gcagggtcac acccgccgcg ataagcggtt
    15361 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    15421 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    15481 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    15541 ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgt ccactgctca
    15601 cgcgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    15661 tccagtagtt cgtgaacagt ttgcccggtg gcgcactgca tcggttgatc gttaaacagg
    15721 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15781 ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggtgtct ctataccgct
    15841 taataactta atggcttcca gtgcctgcaa agtgcccata accccgacca ccgggccaac
    15901 cacgcccgcc gtgcggcagt tgcgttctgg ctcctggtta tctggccaca ggcagcggta
    15961 acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc cgaccgcgct
    16021 ggcggtgata agcggcgtgt tgagtaccac gcaggcggca ttaatctcct ggcgagtcgc
    16081 catattgtcg gtacagtcga gcaccacatc ggcccgtgca accgcatctt ttaacgcctc
    16141 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    16201 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga gaatttgtcg
    16261 ttacagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    16321 gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa tcagcacctg
    16381 gctgtcgagc agtttttgct gcccgtcaag agcgatatca tcgagcagga tttggcggct
    16441 ataacgcata aaatcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    16501 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgat gacggcgata ctgccgacac
    16561 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    16621 aatccgccag tcgctcaaca tgccgtgcca gctgttccag cccctgcggt gcagaaggca
    16681 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16741 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacacc cagccgcagg cctgccgcgc
    16801 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16861 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16921 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16981 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    17041 ccactgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    17101 ttcctcctta cgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    17161 cattcccatt tcaatagttt gcgtggcggc gtaatcacgc acttcctggc tgattttcat
    17221 cgagcagaat ttcggcccac acatggagca aaaatgggcg actttacctg actcttgcgg
    17281 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    17341 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    17401 atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa gcccctgctt
    17461 aacatcttct ttattaggca gacccagatg ctcttttggt gttacgtaac agagcatcgc
    17521 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    17581 cgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    17641 ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc cttcaatcat
    17701 cacctgcacg tcatattccc aggcaatttt ggtcagttcg cccagcgtat gcagctcggc
    17761 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17821 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17881 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17941 accggtcagg cgtttcgcgg tcatcggcac atagcgcaac agtacgcccg catggatagt
    18001 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    18061 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    18121 cggcaccgga ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    18181 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    18241 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaagtt
    18301 acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    18361 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gacgtgcgcc
    18421 aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgcgct cgcggcccat
    18481 attctcgcgg atggcgatga attccatttc cggtgtgatg atgccctggc gggcgtagtg
    18541 cagttgggtg acacggcgtc ctgctttggc gcgttttggt gttagtacgc cgctaaaacg
    18601 cagttcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac tgcgcacggt
    18661 aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18721 ctgcacgtta atggcgatct gcggatcgcc atatgggccg gaggtgtcgt agaccggaat
    18781 cgcttcgttt tcttcaaact gcggctgttc tttgctgccg ccgattagcg tcgggctaag
    18841 ctggatctca cgcatcggca cgcgaacgcc agagtgtgtg ccagtgaggt aaatgcgttt
    18901 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18961 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    19021 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    19081 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    19141 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    19201 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    19261 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    19321 aaaacctttt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    19381 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    19441 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    19501 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    19561 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    19621 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    19681 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    19741 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    19801 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    19861 ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19921 gcactacaaa tcggcgaatg gcagggggaa cctgtttggt tagtacaaca gcagcggcgt
    19981 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgtgctgtt tcaactggcc
    20041 ggacgaggcg tacaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    20101 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg ccgtgagcgt
    20161 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgatcctc
    20221 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    20281 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    20341 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    20401 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    20461 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    20521 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    20581 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    20641 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    20701 tatctaccgg aatataaagt cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    20761 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    20821 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    20881 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataag
    20941 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    21001 cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    21061 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    21121 tatgccgatc cgcaggcgct gcacgctcta ctcgataaac tggcgaaaag cgtcactttg
    21181 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    21241 ggtgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    21301 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccgg tcacgctgtt taccaaaggc
    21361 ggcggacagt ggctggaagc gatggcagaa accggttgcg atgcgttggg cctcgactgg
    21421 acaacggata tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    21481 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    21541 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    21601 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacaa
    21661 tatcaccgct aaggagtgat tatggatctc gcgtcattac gtgctcaaca aatcgaactg
    21721 gcttcttctg tgatccgcga ggatcgactc gataaagatc caccggatct gatcgccgga
    21781 gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag cggcgatggt gctgctgaaa
    21841 tatccctcgc ttgagctggt ggagtataaa gttgcccgca tcgccaccac catgccttac
    21901 attccaggtt ttctttcctt ccgcgaatat cctgcgctgc tggcagcgtg ggagatgctg
    21961 tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga tctcgcatcc tcgccgtctt
    22021 ggcgtcgcca gccattttgg tttgatggtg gatgtgccaa cgatcggcgt ggcgaaaaaa
    22081 cggctctgcg gtaaattcga accgctctcc agcgaaccgg gcgcgctggc cccactgatg
    22141 gataaaggcg agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa cccgttgttt
    22201 atcgctaccg gccatcgggt cagcgtggac agcgcgctgg cgtgggtaca acgctgcatg
    22261 aaaggctatc gtctgccgga gccaacgcgc tgggcggacg cggtggcctc ggaacgtccg
    22321 gcgttcgtgc gctatacagc aaatcagccc taattcaggt aaactgcgga taatttccgt
    22381 atttgagaac ttaacatgtt acaaaaccca attcatctgc gtctggagcg cctggaaagc
    22441 tggcagcatg tcactttcat ggcttgctta tgcgaacgca tgtaccccaa ttacgccatg
    22501 ttctgccagc aaaccggttt tggtgatggg caaatttacc gtcgtattct cgatctcatc
    22561 tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg acagccaact ggagaaattt
    22621 gaagaagcga ttccttcagc cgacgatttc gatctgtacg gcgtttatcc ggcaatcgat
    22681 gcctgcgtgg tgttaagtga actggtccat tcgcgtttga gtggtgaaac gctcgaacac
    22741 gcggtggaag tgagtaagac ctccatcacg accgttgcga tgctggaaat gactcaggtt
    22801 ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag ctgtagagca agaatgggac
    22861 attcagtggg aaatattccg acttttagct gagtgcgaag aacgcgatat cgagctgata
    22921 aaaggcctta gggcagacct gcgtgaggcg ggtgagagca atattggtat aatttttcag
    22981 caataagacc agaaaacgtg atttaacgcc tgatttgtcg tacctggagt cttccctttc
    23041 gccccccgtc tggtctacat ttggggggcg aaaaaaagtg gctatcggtg cgtgtatgca
    23101 ggagagtgct attctggcat ttccgtcgca ctcgatgctt agcaagcgat aaacacattg
    23161 taaggataac ttatgaacaa gactcaactg attgatgtaa ttgcagagaa agcagaactg
    23221 tccaaaaccc aggctaaagc tgctctggag tccactctgg ctgcaattac tgagtctctg
    23281 aaagaaggcg atgctgtaca actggttggt ttcggtacct tcaaagtgaa ccaccgcgct
    23341 gagcgtactg gccgcaaccc gcagaccggt aaagaaatca aaattgccgc agctaacgta
    23401 ccggcatttg tttctggcaa ggcactgaaa gacgcagtta agtaagattg cgtggcagtg
    23461 aacagtttta acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg atcattgatg
    23521 ctggctggcg ctctgcttct cactgcctgt agtcataact cttcacttcc tccctttacc
    23581 gccagtggat ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga cagcggcgat
    23641 aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg gcgataccac gacgcgagag
    23701 tatcgctggc agggcgataa cctcacgctc atcaatatca atgtttacag caaaccgccg
    23761 gtgaatattc gcgcgcgttt tgacgatcgc ggtgatctga gctttatgca acgtgaatcc
    23821 gatggggaaa agcagcagct ttctaacgac caaatcgatt tataccgtta tcgtgctgat
    23881 cagatccgcc agattagcga tgccttacgt caggggagag tcgtgctgcg ccaggggcgc
    23941 tggcatgcga tggaacagac cgtgaccacc tgcgaagggc aaaccattaa acctgattta
    24001 gattcgcagg cgatagcgca tatcgagcgc cgccagagcc gctcttctgt tgatgtcagc
    24061 gtggcatggc tggaagcgcc cgaaggttcg caattactgt tagtggcaaa ctctgatttc
    24121 tgtcgctggc aacccaacga gaaaacgttc tgatttacca gtggcccata cccatatgac
    24181 cgccaccacc gcagccgccg tagcccatgc ccattccggc tccgcgcgga atacccgctt
    24241 cagccatcgc gatatctcgt ttcacccgta actcatctaa cgactgacgc aaattctcca
    24301 tctctttggc gaccgcgtta attttgctgc tatccggtgg gttcgcggct aacagggcat
    24361 tgtattcata acgcttcgtc accagttgct gttgcagtgc gctgctttga gcgtaaaagt
    24421 cattatggat tttctgccac gctgtctgtt gttcgctggt caaaggcgcg gcattttgct
    24481 gccacatacc gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc attgctgaaa
    24541 gcgccatcat taccagggca attttcgtgt tccgtttcat ggttaatcct ccagtggttg
    24601 tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa cgctgatatg acgggtaatc
    24661 tggcatgata aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca tttttactca
    24721 ttgaaactta agcctttgtg ttacagcgca gggtaagcgc tgataaaaga tggcatgatt
    24781 tctgctgtca gaaagggatg agcaggcaaa gaagaagatg cgttttatgc aacgttctaa
    24841 agactcctta gctaaatggt taagcgcgat cctccccgtg gtcattgttg ggctggtagg
    24901 gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag actgccgccg ccagacaaac
    24961 gctgctggaa aaaggcagtg tacttattcg cgctcttgaa tccggctcgc gcgtcggcat
    25021 ggggatgcgc atgcatcatg cgcagcagca ggccttactg gaagaaatgg ccgggcagcc
    25081 tggagtacgt tggtttgcgg tcacggatga acaaggaaca atcgtgatgc atagcaactc
    25141 cggcatggtg ggaaaacagc tttattcccc gcaggaaatg cagcagttac atccgggaga
    25201 tgaagaagcg tggcggcgga tcgatagcgc agacggtgag cctgttctgg aaatttatcg
    25261 ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg cgccatatgc agcagtatgc
    25321 cgcgacacca caagcaattt tcatcgcttt cgacgccagt aatattgtga gtgccgaaga
    25381 tcgtgagcag agaaacaccc tgattatcct cttcgccctg gcgacggtct tgctggcaag
    25441 cgtattgtca ttcttctggt atcgccgcta tctgcgctcg cgccagcttc tacaagatga
    25501 aatgaagcgc aaagagaagc tggtggcact ggggcatctg gcggcaggcg ttgcccacga
    25561 aatccgtaat ccactttcct caattaaagg gctggcgaaa tactttgccg aacgcgcgcc
    25621 agcaggggga gaagcgcatc aactggcgca ggtgatggcg aaagaagccg accgtttaaa
    25681 ccgcgtggta agcgagttgc tggaactggt taagccaacg catctggctt tgcaggcggt
    25741 ggatctcaac acgctgatta accactcatt acagctggta agccaggatg caaacagccg
    25801 ggagatccag ttacgcttta ccgccaacga cacattaccg gaaattcagg ccgatccgga
    25861 caggctgact caggtcctgt tgaatctcta tctcaatgct attcaggcga ttgttcagca
    25921 tggcgtgatt agcgtgacgg tcagcgaaag cggcgcgggc gtgaaaatca gcgttaccga
    25981 cagcggtaag ggaattgcgg cagatcagct tgaagccatc ttcactccgt acttcaccac
    26041 caaagccgaa ggcaccggat tggggctggc ggtcgtgcat aatattgttg aacaacacgg
    26101 tggtacaatt caggtcgcaa gccaggaggg aaaaggctca acgttcaccc tctggcttcc
    26161 ggtcaatatt acgcgtaagg acccacaagg atgacgcacg ataatatcga tattctggtg
    26221 gtggatgatg acattagcca ctgcactatt ttgcaggctt tactgcgcgg ctggggctat
    26281 aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc aggtgcggga acaggttttt
    26341 gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg gcatcgccac gctgaaagag
    26401 atcaaagcgt taaacccggc aattccggtg ctgattatga ctgcgtactc cagcgtcgag
    26461 acggcggtag aggcactgaa aactggggcg ctggattatc tcatcaagcc gctggatttc
    26521 gataacctac aggcgacgct ggaaaaggcg ctcgcgcata cgcacagtat tgatgctgaa
    26581 acacctgcgg tgactgccag ccagttcggt atggtcggta aaagcccggc gatgcaacac
    26641 ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca cagtgctgat ccacggcgat
    26701 tccggcaccg gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc acgtagcgaa
    26761 aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga atctgaattg
    26821 ttcggtcacg aaaaaggggc gtttactgga gccgataaac gccgggaagg gcgctttgtt
    26881 gaggcggacg gcggcacgct gtttctcgat gaaattggcg atatctcgcc gatgatgcag
    26941 gtgcgtctgc tgcgtgcgat tcaggagctc gaagttcagc gtgtcggtag caaccagact
    27001 atctcggttg atgtccggct gattgcggcg acccatcgcg atcttgccgc agaggtgaat
    27061 gccgggcgtt ttcgccagga tctctactat cgcctgaatg tggtggcgat tgaagtacca
    27121 tcgctgcggc aacggcggga agatatccct ctgctggctg gccattttct gcagcgcttt
    27181 gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc aggcgatgga tctgttgatt
    27241 cattacgact ggccgggaaa tattcgtgag ctggaaaacg cggtggaacg ggcagtggtg
    27301 ctgctgccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc agtacgccga
    27361 tcccgctggg gcaaagtcag gatattcagc cgctggtgga agtggaaaaa gaggtgattc
    27421 tggcggcact ggagaaaacg ggcggcaaca aaactgaagc cgcccgtcag ttagggatca
    27481 cgcgtaaaac gttattggca aaactgtcgc gttagttctg ctcgcgttcg atagcacgcc
    27541 agccgatatc tttccggcag aagcagtcgt cccagtggat atcggtcatt aaggcatagg
    27601 cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt acgcgcccgc
    27661 cgttggtcac tacctgctcg tcatccgcca gttttgtgcc cgcgtggaac actttgccgc
    27721 ctgccacttc ttccagcagc aggccgtgga tcacgtcacc ggtgcggtaa tcacccggat
    27781 atccacccgc agccatcacc acgccgagag aagcgcgttc atcccactcg gacgttttct
    27841 cgtccagttt gctttcacag gccgccaggc agagctcaac cagatcggac ttcatgcgca
    27901 gcataatcgg ctgggtttcc ggatcgccaa agcggcagtt aaattcaata accttcggat
    27961 tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg
    28021 ccgccatgcc tttcacggtt ggccagataa tacgttccat ggtgcgctga tgaacgtcat
    28081 cggttactac cggcgcgggg gagtaagcgc ccatcccgcc ggtgtttggt ccggtatctt
    28141 tatcgcctac gcgtttgtga tcctggctgg tagccatcgg cagcacatgc tcgccgtcca
    28201 ccatcacgat aaagctcgct tcttcgccat cgaggaactc ttcgataacg atgcgatgac
    28261 ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcc gcttcttcca
    28321 gcgtcatcgc cacgataacg cctttcccgg cagccagacc gtccgcttta atgacgattg
    28381 gcgcgccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag ttctggtatt
    28441 ccgccgtagg gatcttatgg cgggccagga aatctttggt aaacgctttt gagccttcca
    28501 gttgggccgc acctgcggtt gggccgaaga ttttcagccc ggcggcgcgg aaggtatcga
    28561 ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc ttttcgtttt
    28621 gtgcgaaatc caacagcgcc gggatatcgg tcacgccaat agcaacgttt tgcagcgcgg
    28681 gttccagcgc agtgcctgca ttacccggag caacaaaaac agtctcaacc agcggcgact
    28741 gggccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact aatactttca
    28801 tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg caataccgtg
    28861 ctcgtcggcg gcggcaatca cttcgtcatc acggatggaa ccgccaggct ggattacgca
    28921 ggtcacgccc gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga
    28981 agccatcgag gaacctttca cttccaggcc ttcatcggca gctttaatac cggcgatttt
    29041 cgcggagtac acgcggctca tctggcccgc gccaataccg atggtcatat tgtttttggc
    29101 atagacgata gcattggatt tcacgaactt cgccactttc cagcagaaca gcgcatcgcg
    29161 cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg caccgaccat
    29221 ccccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat caagacccgg
    29281 aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat
    29341 tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact gacgagaaat
    29401 gatggcctgt gcggtttccg catctagctc gcggttaaag gcaatgatgc cgccgaatgc
    29461 ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat tgccgatagc
    29521 tacaccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga actctttcac
    29581 gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt taccctgaac
    29641 ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga aggcagcctg
    29701 ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca ggttcagcgt
    29761 gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg tacgccggaa ccatgctgcc
    29821 gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag
    29881 gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt taataatggc
    29941 gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag cggcggagcg
    30001 caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg agcagccttc
    30061 acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga taggctggat
    30121 ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc caccatgtac
    30181 tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga
    30241 aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc ctgtagacag
    30301 cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga
    30361 aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc ccctggattt
    30421 gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg
    30481 gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt
    30541 ttctctttca atggtgatca caattttgac agtggctacc gtgggaaaaa tacagaaatt
    30601 acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg
    30661 aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga gaactcccta
    30721 taatgaacct ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt
    30781 gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta atatacgcca
    30841 cactcacgac agtgcgctaa agctcgtcgc aactgctctt taacaattta tcagacaatc
    30901 tgtgtgggta ctcgaagata cggattctta acgtcgcaag acga
//
LOCUS       DAJTOR010000031        30186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTOR010000031 DAJTOR010000000
VERSION     DAJTOR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.031"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..869)
                     /locus_tag="OO249_001244"
                     /pseudo
     CDS             complement(1..869)
                     /locus_tag="OO249_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(844..1644)
                     /gene="otsB"
                     /locus_tag="OO249_001245"
                     /pseudo
     CDS             complement(844..1644)
                     /gene="otsB"
                     /locus_tag="OO249_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(1811..2797)
                     /gene="araH"
                     /locus_tag="OO249_001246"
     CDS             complement(1811..2797)
                     /gene="araH"
                     /locus_tag="OO249_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5635612.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2812..4326)
                     /gene="araG"
                     /locus_tag="OO249_001247"
     CDS             complement(2812..4326)
                     /gene="araG"
                     /locus_tag="OO249_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5635613.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGAIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(4396..5384)
                     /gene="araF"
                     /locus_tag="OO249_001248"
                     /pseudo
     CDS             complement(4396..5384)
                     /gene="araF"
                     /locus_tag="OO249_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            6098..6601
                     /locus_tag="OO249_001249"
     CDS             6098..6601
                     /locus_tag="OO249_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5635614.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6680..6931)
                     /locus_tag="OO249_001250"
     CDS             complement(6680..6931)
                     /locus_tag="OO249_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5635615.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(7046..7132)
                     /gene="azuC"
                     /locus_tag="OO249_001251"
     CDS             complement(7046..7132)
                     /gene="azuC"
                     /locus_tag="OO249_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5635616.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7395..7718
                     /locus_tag="OO249_001252"
     CDS             7395..7718
                     /locus_tag="OO249_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5635617.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7889..8386
                     /gene="ftnA"
                     /locus_tag="OO249_001253"
     CDS             7889..8386
                     /gene="ftnA"
                     /locus_tag="OO249_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5635618.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8424..8663)
                     /locus_tag="OO249_001254"
     CDS             complement(8424..8663)
                     /locus_tag="OO249_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5635619.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            8854..10065
                     /gene="tyrP"
                     /locus_tag="OO249_001255"
     CDS             8854..10065
                     /gene="tyrP"
                     /locus_tag="OO249_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5635620.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVR"
     gene            complement(10127..10792)
                     /gene="yecA"
                     /locus_tag="OO249_001256"
     CDS             complement(10127..10792)
                     /gene="yecA"
                     /locus_tag="OO249_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5635621.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10988..11074)
                     /locus_tag="OO249_001257"
     tRNA            complement(10988..11074)
                     /locus_tag="OO249_001257"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11038..11040),aa:Leu,seq:taa)
     gene            complement(11087..11160)
                     /locus_tag="OO249_001258"
     tRNA            complement(11087..11160)
                     /locus_tag="OO249_001258"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11126..11128),aa:Cys,seq:gca)
     gene            complement(11215..11290)
                     /locus_tag="OO249_001259"
     tRNA            complement(11215..11290)
                     /locus_tag="OO249_001259"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11255..11257),aa:Gly,seq:gcc)
     gene            complement(11442..11990)
                     /gene="pgsA"
                     /locus_tag="OO249_001260"
     CDS             complement(11442..11990)
                     /gene="pgsA"
                     /locus_tag="OO249_001260"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5635622.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCGAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(12047..13879)
                     /gene="uvrC"
                     /locus_tag="OO249_001261"
     CDS             complement(12047..13879)
                     /gene="uvrC"
                     /locus_tag="OO249_001261"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5635623.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEDEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13876..14532)
                     /gene="uvrY"
                     /locus_tag="OO249_001262"
     CDS             complement(13876..14532)
                     /gene="uvrY"
                     /locus_tag="OO249_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5635624.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14827..15003
                     /locus_tag="OO249_001263"
     CDS             14827..15003
                     /locus_tag="OO249_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635625.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            14990..15214
                     /locus_tag="OO249_001264"
     CDS             14990..15214
                     /locus_tag="OO249_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5635626.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(15281..16003)
                     /gene="sdiA"
                     /locus_tag="OO249_001265"
     CDS             complement(15281..16003)
                     /gene="sdiA"
                     /locus_tag="OO249_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR5635627.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(16233..16985)
                     /gene="yecC"
                     /locus_tag="OO249_001266"
     CDS             complement(16233..16985)
                     /gene="yecC"
                     /locus_tag="OO249_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5635628.1"
                     /translation="MSAIEVKNLVKKFQGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(16982..17650)
                     /gene="tcyL"
                     /locus_tag="OO249_001267"
     CDS             complement(16982..17650)
                     /gene="tcyL"
                     /locus_tag="OO249_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5635629.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPILSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17665..18651)
                     /gene="dcyD"
                     /locus_tag="OO249_001268"
     CDS             complement(17665..18651)
                     /gene="dcyD"
                     /locus_tag="OO249_001268"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5635630.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLNLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18756..19556)
                     /gene="tcyJ"
                     /locus_tag="OO249_001269"
     CDS             complement(18756..19556)
                     /gene="tcyJ"
                     /locus_tag="OO249_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5635631.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19644..20195)
                     /gene="fliZ"
                     /locus_tag="OO249_001270"
     CDS             complement(19644..20195)
                     /gene="fliZ"
                     /locus_tag="OO249_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5635632.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQAHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(20241..20960)
                     /gene="fliA"
                     /locus_tag="OO249_001271"
     CDS             complement(20241..20960)
                     /gene="fliA"
                     /locus_tag="OO249_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR5635633.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLSTKLGKL"
     gene            complement(21280..22986)
                     /locus_tag="OO249_001272"
     CDS             complement(21280..22986)
                     /locus_tag="OO249_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5635634.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRILELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSAKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGAGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLL
                     QG"
     gene            23252..24658
                     /gene="fliD"
                     /locus_tag="OO249_001273"
     CDS             23252..24658
                     /gene="fliD"
                     /locus_tag="OO249_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5635635.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAKNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            24683..25093
                     /gene="fliS"
                     /locus_tag="OO249_001274"
     CDS             24683..25093
                     /gene="fliS"
                     /locus_tag="OO249_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5635636.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            25093..25459
                     /gene="fliT"
                     /locus_tag="OO249_001275"
                     /pseudo
     CDS             25093..25459
                     /gene="fliT"
                     /locus_tag="OO249_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            25537..27024
                     /gene="amyA"
                     /locus_tag="OO249_001276"
     CDS             25537..27024
                     /gene="amyA"
                     /locus_tag="OO249_001276"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5635637.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADVFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYNLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(27058..27471)
                     /locus_tag="OO249_001277"
     CDS             complement(27058..27471)
                     /locus_tag="OO249_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5635638.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLALMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            27658..28863
                     /gene="yedE"
                     /locus_tag="OO249_001278"
     CDS             27658..28863
                     /gene="yedE"
                     /locus_tag="OO249_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5635639.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            28860..29093
                     /gene="yedF"
                     /locus_tag="OO249_001279"
     CDS             28860..29093
                     /gene="yedF"
                     /locus_tag="OO249_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5635640.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            29200..29871
                     /locus_tag="OO249_001280"
     CDS             29200..29871
                     /locus_tag="OO249_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR5635641.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(29911..>30186)
                     /locus_tag="OO249_001281"
     CDS             complement(29911..>30186)
                     /locus_tag="OO249_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5635642.1"
                     /translation="EYKQLWRGGQVFAVPPACTSQRCACCGHTAKENRLSQSKFRCQV
                     CGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         7494 a         7770 c         7231 g         7691 t
ORIGIN      
        1 ataacgaatt ttaccatgat gctgcggata tttttccagc aacgcttcat aggcgagaaa
       61 acgctctggc aaacctttgg aataatccag ccgttcgaca gaaaagatat tttgtacgtt
      121 tttcagttcc gctttaagtt gcgccagttt tggcggcagt ggcccggcag cctgtttggc
      181 tatttctttc ggttcaatgc cgatcgggta gacttctgtt cgaaatgctt tgccccaggc
      241 tgtatggctt tttgcgctac gtgtcgtgac gcgggtcagg ttagaaagac aatccaggaa
      301 cgccagacga tcgttttctg tctggaaacc cagcaaatca taatcacaaa gctgttcaag
      361 caaggtgtca tatgtcggca gcgcgttgaa gatttccggt gtcgggaaag gaatatgcag
      421 aaagaaacca atgcgattat tcaccccacg tttgcgtaat tcatgcgcaa agggcaacag
      481 gtgataatcg tggatccaga taatgtcatc gtcttgcaac agcggcagta atttatctgc
      541 cagcaacgca tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcatcagatc
      601 gagccgataa tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc
      661 aaggtcctgt tcgctgaggt taaaagaggc ccacgtaatg ttaccttttt tagcggctga
      721 tcctcattcc ctgtttcacc actccagcca aaccacagtc cgcctgtggc tttcagtgcc
      781 cccagtatgc caacggcaag gccaccggca ctggcggcgt gctcgtctgg tggtgcaatc
      841 cggttagata ctacgactaa acgactcata gtcatcactc ctgttatttt ctcttttttg
      901 ttgtaatgcg gtggttatca tttcaagcct gctccagaca tccggcacac ccgccagtcg
      961 ccatgatgcc tgagttgcac ctgtgccaat ttttactgac attccgccca gtcggttaac
     1021 gactgcgaag ccagattcat cggttaaatc atcgcccaga aatacgggcg ttcgcccgat
     1081 aaagggagct tcctgcataa aagctgcaat tgcctcacct ttactggtac ctctcggttt
     1141 gatctcgaca acacactttc cctgctgtaa cgccatttgt ggccagatct gagtaatacg
     1201 ttacgctaat gtcattaatg cgtcttcatg ctgcggagcc tgacgataat gcagcgcaaa
     1261 agccatccct ttcgcctcca gctccgcgcc gggatactga gcgataactg tatgcagttg
     1321 cacgctaata tcacgcgcaa tcgcatccgg cagatgaacg atatgcgttt taccattgat
     1381 gtcacggcgc tccgccccat gcacgcccgc taacgggaag cgataaggtt ttgccagtgc
     1441 gtcaagctcc accattgagc gccctgatat caatgccaat gcaccatcac ttgcggttgc
     1501 cagtagctgt agtccttgca gaatattgtc aggcacgacg acctgatcgg gatgcggttt
     1561 gatttccgcc agcgttccat cgagatcaaa aaaccaggca tatttcgcgg atagttcagg
     1621 ggtttcggtt aacggttctg tcacccggtt ctcctacttc tcttttctta ttgttttgcg
     1681 cagaacgcgc tttacagaca tgtaagtata gacagtgtga cgggggtcgc catttgcaac
     1741 gaagaacagc caaccgttat ggcggttggc tgtggtggga aaaaacgcta aattgttgca
     1801 gaaaaaagca tcagacagtg cgtttcgctt tttgcttgta acggtcgaag atcaccgctg
     1861 ccagcaggat taagccgcga accacgtact gcgcgaaagg agaaatatta agcaggttca
     1921 tggcgttttc cacggtgcct aaaattaaga tacccgccac cacatatgag atttttccga
     1981 tgcctccttt cagagaaacg ccacctaaaa cacaggcgga gataacaatc agctcgtaac
     2041 caatcgacgt cattggctgc ccactggtca tacgtgaagc cagaataatt ccggctatcg
     2101 cagataccag gccagagaga acaaagataa taattttggt gcgaacaacc ggtacacccg
     2161 ccagacgcgc agcctcttcg ttcccgccaa tcgccagggt gttacgacca aaggtggttt
     2221 tattcagcaa caaaccaaag ataatcagac acgcgacggt gagccagatt ggcgcaggca
     2281 gaccgaacca gttagcgtaa ccaagggcaa agaagctttc atcttcgata ccgaccgctt
     2341 taccgtctga aatgatgtac gccagacctc gaacaatctg catcgttgcc agtgttgtga
     2401 tcagagcatt tattttcagt ttggcgataa caaagccatt gaccaggcca cagagaatgc
     2461 caagcagcaa ccccgctgcc acgccaatcc acaggctttc agtcaggttg ataaccaccg
     2521 ccgtggtgac acccgcacag gcaattacgg aggcgacaga aaggtcaaag tcaccggaag
     2581 caaggcagaa caacatgcca cacgccacca tccccgacat ggaaattgcc aggcccaacc
     2641 ctttcatatt aatgaaggtg gcaaaatttg ggacaaaaat ggcacaggcg ataaagagca
     2701 ccgcaaacac caccagcatg ccgtactgat cccagatacg cccgaagctg aatgacgact
     2761 taggtgcgcc agaacccgat gtagaaacag aagacatcat actctcctta ctcaggcaac
     2821 tgcctggctg actttaggca tcgcaaggct cagtgcctga cgctcatctg cctgctcgtg
     2881 taacaattca ccggcgattg caccttcccg catcaccaca atccggtcgg caacgccgag
     2941 gacttcaggt aagtcgctgg aggcaaacag caccgccaca ccctgcgccg ccagcgcata
     3001 aattacgtta tatatttcgt gcttagcacc aacatcaatg ccgcgcgtag gttcatccag
     3061 caaaatgacc ttcatctctt ccgataacca gcggcccaga atggcttttt gctgatttcc
     3121 gcctgagaga ttcatgatca gttgctcagc gcccggcgtt ttgatgttga gcgaacgaat
     3181 gtgctgatcg gcattgtttt cttcccaacc gttgttgatt acacaaccgc cgagcacatg
     3241 tttgcgtctg gcactgatgt tgatattgtc gcgaacggag tgcacgggaa taatgccttc
     3301 cgctttgcga tcttccgggc agagcatcat gcctgcggca atggcgtggc tcggtttacg
     3361 aatatcgatc ggctgttggt cgatataaac ctgaccggcg gtgatttgcg tcccgccaaa
     3421 caagcctttc attaattcgc tacgccccgc ccctaccaga ccaaacagcc caacaatttc
     3481 accactgcga accgccagac ttattggcgt acgcacgcct ggtgctttca cagcatcaag
     3541 acgcaggcgc tcctcgccat aactacgagg ttgccagccg tagatatcgc caatgtcgcg
     3601 cccgaccatc gcctgcacca gcgcgtcgtg gtcaacctgc tgcatatcgg taaaggtttt
     3661 gacataacgt ccatctttaa agacagtgat ggcatcgctg agggcaaata tttcttccat
     3721 acggtgagaa acgtataaga tcacccgccc ctcttttcgc agttcacgaa taacgcggaa
     3781 aagattgtcg atttcgcggg cagaaaggga gctggttggc tcatcaaagg cgataatttt
     3841 agcgttacgc gccagcgcct tggcgatttc aaccatctgc cactgaccaa tggagagata
     3901 tttcagcggc gtgtccgggt caatatccat accaagatgt ttaagttgta aacccgcctc
     3961 ataattcagc aatgagcgat tcacaatgcc gcctttatgc ggcagctggc cgagatagat
     4021 gttttccgcg acggtcattt ccggcacgag atgcagttcc tggtaaataa tcgccacacc
     4081 cgcgttaagt gctgcggtcg tgtcggaaaa ggacatttcc tgcccattaa tcactacaga
     4141 acccgtggtt ggcgcatagt tgccgctgag gatttttaag agagttgatt ttcctgcgcc
     4201 attttcaccc atcaacgcat gaacctgacc ggcatagcag tcaaaactaa tatccgtcag
     4261 cgccttaacg ccgggaaacg ttttaccgat gccgcgaaat gagagatacg gggtagactg
     4321 ttgcataacg tctccgtgaa tcactggtcg ttcacacccc ctctgcatca tgcagagggg
     4381 aatttttccg gcaaattact taccgcctaa accttttttc tccagttctt ctttaaagtt
     4441 gtcacgcgtg atcagcacca cgtcggtaac ttcggtaaat tttggcggtt caacgtcttt
     4501 tgctacccag ttgtaaagca tttcgctgga tttatagcca tgtacgtccg ggcttggcag
     4561 cagggaaccg tagaagccgg ttgcctgtac tttagacagt tcgctcaccg catccacacc
     4621 gttaatgcca atgccgataa tatcggcagc tttaaagccc tgaccttccg tcgcgcgtac
     4681 gccgcccagc acggtgctgt cgttcatacc gacgatcagc caatgtttaa cttcaggatg
     4741 ttgaaccagc attgagttgg cagcgtcaaa tgcccccggg atgtcgttag atttggtagg
     4801 tacctgataa atttgttttt ccgggaatcc ggccgctttc agcgcatcca tagatcccgt
     4861 agtacggcgg cgggcagtat ccagttcgtt ggcggtaatc accatcaccg cgctttcttt
     4921 gacatcccag ccacgtttct gcatctcttt atacagttcc tgaccctgac gttcgccaat
     4981 tttagtcgcg ccatcatcac cagcggaacg gtatccattg gcttaccttt ggcgttaaca
     5041 aactgatcat ccacggcaat gactttcata tcgtagccac gcgctttcgc gacgatggca
     5101 gagccgagtt tggggtccgg agtacaaata acgaaacctt ttgcgccact ggcagccagg
     5161 ctgtcgatcg cgttcaatgt tttttcgcca tccggcacgg caatcttaat aacctcaaac
     5221 cctaaatcct tcccggcttt atcggcaaac ttccattcgg tctggaacca cggctcttcc
     5281 ggttgcttca ccagaaaacc gagcttcagg ttctccgcca tagcggattg tgacataacg
     5341 gctgccagac caatggctgc cagggcttta gtaaatttgt gcatggttct ctccagcttt
     5401 agtgtcgttt tgtgtagggc aaaaacgaat gacattcgtt aaattaatcg gaaaacaaag
     5461 cattaccttt taactaaaag ataagtgacc gtgttgacat agttttagcg agaaattaat
     5521 tctccatagg agagcaatat cacatcgcag aattacagtg agaacgtgca taaatttagc
     5581 gggaaaagac ataagggaaa gccaatttgt cagacaaatt gtcgaatgca cagcagatta
     5641 atccataaga ttagcttgga aatccttgtt gtctttggta cccatgcggg atgtcttctt
     5701 tttaaccagt caataggccg cattacctgg cgttgagttt ttgaaatggt gtaataaccg
     5761 caactcaaag atgtggaaaa tgcacgtcat tcatttcgcc attaattatc actgtgctca
     5821 ttaattaaca gaacacgtat aatgagagcc atctcgcaaa aatgaaaaaa cgttttataa
     5881 aatcatcatt tcatcaatga aatgtaaaaa tatataaact tgatgattta agcattttct
     5941 tatacccgtt cagacgttat tcttatttca gatcatcgtc agaattgact ccacgatcac
     6001 atttcggacc ggcagaaagg aattattctg caaacagtaa ttatggtgtt ttgatttatc
     6061 ttgcacctct ccacttctgg atataaggat attaggtatg gcaaccgctg gaatgcttct
     6121 caaactcaac tctcaaatga accgcgagtt ttacgcatcc aatctctacc ttcacctgag
     6181 taactggtgt tctgaacaga gtctgaacgg caccgccact ttccttcgcg cccaggcaca
     6241 gagtaatgtg acccaaatga tgcgcatgtt taactttatg aagagtgtcg gcgctacccc
     6301 catcgttaaa gccattgatg ttcccggtga aaaactgaac tctctggaag aactgttcca
     6361 aaaaacgatg gaagaatacg agcaacgttc tagtacgttg gcacagttag ccgatgaagc
     6421 gaaagaactg aatgatgatt caaccgtcaa tttcctgcgc gatctggaaa aagaacagca
     6481 gcatgatggt ctgttgctgc aaaccattct tgatgaagtg cgcagtgcga aacttgctgg
     6541 tatgtgccca gtgcagaccg accaacatgt tctgaatgtc gtgtcgcatc agctgcattg
     6601 atcatacatc ggcgctaatg cattgcgccg atgaagtttt tgagaaaccg ctgcctcatc
     6661 tttttgaagc agcggttttt taatgggatt cgccttgtgg ggtaaacttg agttcaatca
     6721 gcgcgatggc tttttggatt gcccgcatgg tgaccgggtc tgcggcggcg ggatggttac
     6781 taaagtcgat attcttcagc tgactggaca ttttttcacg aacttcaacg ggcgcgatta
     6841 catcgagaac atccagaatt tgtttgataa ccaactggca agcaaccaca tcagaaacca
     6901 gttcctgatc ggcattcagc ggctgggaca tcgtaaactc ctgatagcat tttgaaaacc
     6961 gttatagtag cggcttcaca tcttcagcga tagtcacatc caccgtcatc aggacacaaa
     7021 aaaacctgcc ggagcaggtt ttttgttatc ggaacatatt gcctggcggt acgtctttga
     7081 acgtcttgca atagttattg aacatacttt tcaggatttt gcgcagtttc atcgcggcac
     7141 tccgaccatt tgttatacat gtgttatttt ctttgtgctt ataatatgac aaccatcaca
     7201 aaaatcaatc tttatgtgat gcaaatcaca caaatacccc cttaatgtta taaaaatgat
     7261 aatcaaaaaa cagccccccc atttctgaca cctacagatg gcaagaaata gcgcctgcca
     7321 ggcgtctttt ccggtcattg tcgcagcgct gtaacgcgta aaatagtgct ttcttttact
     7381 cttctggctg gaccatgaga cttctgattc tgactctttc attaataacg ctggcggggt
     7441 gtacggttac tcgccaggcc catgtgagcg aagttgacgc ggcaacaggt attgtacggt
     7501 tggtttatga ccaggctttt ttgcagcatg cccataccga tcgttatgtc agtcgcggga
     7561 tcgccgatcg cgcatgccag caggaaggct atacccacgc aatccccttt ggtcagccag
     7621 taggcaactg tagccttttt gcgggttcct tatgtttgaa tacggaattc actttgtcat
     7681 atcagtgcca tcattccgcc ttccctgtct ttctctaaat attttaatca gcgaggggat
     7741 cttcgctgat taaaaaaata gcggtaatgc gttttattcc cattcgtatt tttaataatt
     7801 gaagtttata ttttaccttt tgcaaataat aaaataacaa attatagtga cgccacagca
     7861 acaaatataa cctttgtgga gcactatcat gctgaaacca gaaatgattg aaaaacttaa
     7921 tgagcagatg aacctggaac tgtactcttc actgctttat cagcaaatga gcgcctggtg
     7981 cagctatcat accttcgaag gtgctgccgc gttcctgcgc cgtcacgccc aagaagagat
     8041 gacgcatatg cagcatctgt ttgattacct gactgatacc ggcaatttac cgcgtattaa
     8101 taccgttgaa tctccgtttg ctgaatattc ctcacttgat gaattattcc aggaaaccta
     8161 taaacacgaa caattaatca cccagaaaat taacgaactg gctcatgctg caatgaccaa
     8221 tcaggactac ccaacattta atttcctgca atggtatgtt tctgagcagc atgaagaaga
     8281 gaaactgttc aaatcgatta ttgataaatt aagcctggca ggcaaaagcg gcgaaggtct
     8341 gtattttatc gacaaagaac tctctaccct cgacacacaa aactaatact ctccggcggc
     8401 aggtttactg ccgccgtttc aaatcagtga cggcaaatct tactctgatc ggtcgaaaaa
     8461 ccgtctttcg aaggtataaa tttgcctttt gctgccagaa acgctaccag ctctcccgct
     8521 gtcatccctt ctgccgagca ggtgtgaaaa cgtgcctgtt caccaaaacg cgctttaatt
     8581 gctgcttcca gactggcatg cgtatattgc tcgcctgatt caatcatcat atttaacact
     8641 tcatgaccgt gaatagagtc catcgttcct cctcaaaaaa agcctagcgt agcgattgcc
     8701 gcttatgaag actttgcgcc agagcagaac tgaatgcttt ttattgtaca tttatattta
     8761 caccatatgt aacgtcggtt tgacgaagca gccgttatgc cttaacctgc gccgcagata
     8821 tcactcataa agatcgtcag gacagaagaa agcgtgaaaa acagaactct gggaagtgtt
     8881 tttatcgtgg cgggaaccac aattggcgca ggcatgctgg caatgccgct ggctgcggca
     8941 ggtgttggtt ttagcgtcac gttaatcttg ttgattgggc tttgggcgtt gatgtgctac
     9001 acggcgctat tactgctgga ggtgtaccag catgttccgg cagataccgg tctgggcacg
     9061 ctggcaaaac gctatctggg acgctacggt caatggctga cgggcttcag tatgatgttc
     9121 ttaatgtatg ctctgactgc ggcatacatc agcggtgccg gtgaattgtt ggcctccagc
     9181 atcagcgact ggacaggtat ttcaatgtcg gcaaccgctg gcgtgctgtt gttcactttt
     9241 gttgccggtg gcgtggtttg tgttggaaca tcgctggtcg atttatttaa ccgttttctg
     9301 ttcagcgcca aaattatttt tctggtggtg atgctggtat tgctgctacc gcatattcac
     9361 aaagtgaatc ttttaaccct gccgttgcaa caggggctgg ctctgtcagc aatcccggtg
     9421 atttttacct catttggttt tcacggtagc gtgccgagta ttgtcagcta tatggatggc
     9481 aacgttcgta agctacgcag ggtgtttata accggtagtg cgatccccct ggtggcatat
     9541 attttctggc aggtggcaac gcttggcagc attgattcaa caacctttat gggattgctg
     9601 gctaatcatg ctggattaaa cgggctgtta caggcgttac gcgaaatggt ggcctctccg
     9661 catgttgagc tggcagtgca tttatttgct gatttagccc tcgccacgtc atttctcggc
     9721 gttgcgttag gcttatttga ttatctggct gatttatttc agcgttcaaa taccgttggt
     9781 ggacggttgc aaactggtgc aattaccttt ctgccgccgt tggcgtttgc actgttttat
     9841 ccacgaggat ttgtgatggc gctgggttac gccggtgtgg cgctggcggt actggcattg
     9901 attatccctt cgctgttgac ctggcaaagc agaaagcaca atcctcaggc gggttaccgg
     9961 gtcaaaggtg gtcgtccggc gctggtggtg gtgtttctct gtggtattgc tgtgattggc
    10021 gtgcaatttt tgattgcggc agggttgtta ccagaagtga ggtgatcaga tagcctcaaa
    10081 ttccttattg ggtgccagaa ttaacgctga cacccaattt ggcctcttaa tgcaggcagc
    10141 actgcttaaa tttcttacca ctaccgcacg ggcaaggatc gttacgcccc ggtttctctt
    10201 ctgctttgat cggttgctga acagcttttt cctgcggatg cgccatccag tacgcatgta
    10261 gatcaagcgc cgccagtcga atggcatcta cgctctcttc aaacgcttct ggcgacatct
    10321 tttctacccg ctcgaagttt tcctcagtac cgtgcagcgc aatcgcctcc agcgctggtt
    10381 ttaacgaatc gggcaacgtt gaccagtcag aaagtgccac gccccgcata tagccaaagc
    10441 accactcctc aacaatcgtc agctcgctgc catcaacttc tcgcaagccg aataacggct
    10501 caaactgctc cgggaattcg ttcagacgct ctgcggtatc ggccatatgt tgaaaagcca
    10561 ggttcataaa gcgcgtcatc tctttctctg acgcccagcg cggcacatag tcagccccac
    10621 cccacacggc aaccagccac tgttccggtt caatctcttg cggagaactc aacaccgccg
    10681 tcaataaatc gtccagctcc gccacatcaa ggatggcgtg gttagtgttg tatttggtca
    10741 gaatatcgtc cagccattcc aactcacttt cgtttaacgg tcccgttttc atacgctttt
    10801 ccttgtggat ctcaactcgc cagcacctat cttacatgcc ggtccgtatc agagatactt
    10861 tttgagtggc tttgccggtg attaaaaatt aaggagggtg taacgacaag ttgcaggcac
    10921 aaaaaaacca cccgaaggtg gtttcacgac actgcttatt gctttgattt tattcttatc
    10981 tttcccatgg tacccggagc gggacttgaa cccgcacagc gcgaacgccg agggatttta
    11041 aatcccttgt gtctaccgat tccaccatcc gggctcggga agaaagtgga ggcgcgttcc
    11101 ggagtcgaac cggactagac ggatttgcaa tccgctacat aaccgctttg ttaacgcgcc
    11161 aaattcttca ggcctttcag ccagacatcc gcttgacgcc gatgtctttt aaactggagc
    11221 gggaaacgag actcgaactc gcgaccccga ccttggcaag gtcgtgctct accaactgag
    11281 ctattcccgc attcattaag caatcagtta atcacttgat tttattatcg tctggcaatc
    11341 agtgccgccg ttcgatgcgt tgcattctac ttacctggcg cgatgagtca acgatatttt
    11401 tcaccacttt tgatcgtttg ctgaaaatta cgccgaaacg atcactgatc aagcaaatct
    11461 gcacgcgcag cgctcaaata ttgcaacatt gaccacagag tcagcaccgc agccacaaag
    11521 aaaagtgcaa taccggcgta ctcaacccaa atgttcggac gccacagcag ccaggccaac
    11581 gccaccatct gggcagtggt tttcactttc ccaatccagg agacagccac gctactgcgt
    11641 ttacccaact ccgccatcca ttcgcgtagc gcagaaataa taatttcacg ggcgatcatc
    11701 gttgccgccg gtagggtcac ccaccagctg tgataatgct cggttaccag caccatggcg
    11761 atagccacga gaactttatc tgctacaggg tcaaggaaag caccaaaccg ggtactctgg
    11821 ttccagcggc gtgccagaaa accatcgaac cagtcagtca ccgccgcgcc gcagaaaatg
    11881 agcgcggcgg caaacggcga ccaggtgaca ggcagataaa agaccaatac aaagaatggg
    11941 ataaggatga cacggaacag tgtaagcaac gtagggatat taaattgcat aatgacgggt
    12001 aactatctgt tgtcagtaag attaccccta tgttgctaca gagacatcaa tgtttcaacg
    12061 accagaagat cttttctgcc agaccttgcg aaatacccgg cacttttgca atttcctcga
    12121 cgctggcgtt acgtaaacct tgcaaaccgc ccatatattt caacaacatt tgccgacgtt
    12181 ttggcccgac gccttcaatg gtttccaggg aactggtatt tttgaccttc gcccgttttt
    12241 tacggtgccc gccaatcgcg tgatcatgtg attcatcgcg aatatgctgg ataacatgca
    12301 gcgcgggtga atctggcggc aaactaaatc cctcatcttc cggctcaaag aacagcgttt
    12361 ccagtccagc cttacgatct gctcctttgg caacgccaag tagcagcgga tgatttttat
    12421 cccatgagac atccagttcg gcgaagacat ttttcgcctg cgcaagctgg cctttgccgc
    12481 cgtcgataag gatcacatcc gggatcttac tgtcgtcaat ggctttacca taacgccgac
    12541 gcagcacctg attcatcgcc gcataatcat cgcccggcgt gatgcctgta atgttatagc
    12601 gccgatactc cgcacgcagc gggccgttag catcaaacac cacacaggaa gcgacggttt
    12661 gttcgcccat ggtatggctg atgtcaaagc actccatccg cttcacttcc ggcaatttca
    12721 acacgctggc aagcgcggtc agtcgctggt gaacggtaga ttgctgcgaa agtttgctgg
    12781 ttaaggccgt cgccgcattg gtgcgcgcga gtttcagata acgcgcccta tcgccgcgag
    12841 gtttggtttg aacattaatc ttgcgtcccg ccagttctga aagggaatcg gcgagcagcg
    12901 ttttatcgct aagattaaaa tcgagcagga tctcacccgg taaggtgcgc atctggctgc
    12961 cttgtaaata gaactgacct acgaaggttt ctaccacctc gctcagttcc gtaccgccag
    13021 gcactttcgg gaaatagctg cggctgccga gcactttgcc ctgacgaatg aacaatacgt
    13081 ggacacaagc catgcccgca tcgaacgcca caccaataac gtcaaggtcg tcgccggtat
    13141 tggaaacaaa ctgtttttcg gtgacgcgtc gcaccgcctg aatttggtcg cgaatacgtg
    13201 ccgcttcttc aaactccaga ttctgactgg cagtttccat acggctaatg agttgcgtaa
    13261 gcacctgatc atctttgcca gacaaaaaca ggcgcacata ctcgacctgc tgagcgtatt
    13321 cttcttcact caccagtcct tcaacgcacg gtcccagaca gcgccctatc tgatattgca
    13381 gacacggacg cgagcgattg cgataaacac tattttcgca ctggcgaatg gggaaaatct
    13441 tttgcagtag cgccagagtt tcacgtacgg catagccatt cgggaacggg ccgaaatatt
    13501 cacctttggc atgcttcgca ccacgatgca tcgccagacg cgggtgggta tcgccactca
    13561 ggaagataaa aggataagat ttatcatcgc gtagcaaaac gttgtaacgc ggctgataga
    13621 gtttgatgta gttgtgttcc agcaacagcg cttcggtttc tgtatgagta accgttacat
    13681 caatttgctg aatctgggcg accagcgctt cggttttgcg cgaagcgagg ttgctacgga
    13741 aatagctgga aagccgtttt ttcaggtctt tcgctttgcc gacatagata accgtaccac
    13801 cagcatcgta catgcgataa acgcctggct ggctggttac ggtttttaaa aacgcttttg
    13861 cgtcaaactg atcactcact gacttgataa tgtctccgca ttacacagac catggcgaat
    13921 tgccaggtga gtcagctcaa catcgccatg aatgtttagt ttactgaaca tacgatagcg
    13981 gtagctgttc accgttttcg gactgagatt gagctgttct gagatctcat tgaccttctg
    14041 gcccttggtg atcatcagca taatctgcaa ttcacgttca gacaaactgg caaatgggct
    14101 ttctgttttt tctggttcga tctggcttaa cgccatttgt tgagcgatgt cagaagcaat
    14161 gtaacgctgc cctgaataga cagaacgaat cgcactcacg acttcctgcg gagccgcgcc
    14221 tttgctgagg tagcccgcag caccggcctg catgactttc gctggtaaag ggttttctgt
    14281 atgaacggta agcatgatga ttttgacatc agctgtggaa cgcgcgattt tacgcgtcgc
    14341 ctcaagaccg ccaatgcccg gcatactcat gtccattagc accacgtcaa cggcatttgt
    14401 ccggcaccac ttaacggcgt cttcaccgca cgatgcctca ccgacgactt ttataccctt
    14461 tatatcttcc agaatgcgtc gtatccctgc gcgcaccagt tcgtggtcat caacaagtag
    14521 aacgttgatc aaagaaatat ctccagaaat agggataacg ctactgatag ttagtcattc
    14581 gtatattagc ggtttttatt gcaactttga aacgttaaaa atggtgggta ttcgattttt
    14641 ctctcgtttt tgtcaattcg attgtccaca gttatggtca cgcccgtcac caacggctta
    14701 ccggatcgtt tcacagtaag ccctacgatg aaatgtgaca aaaattgact ttattcagca
    14761 aaaatgaaaa tcagcccgct gaatatgtaa cattaattaa cccgaacaat acgggcaaaa
    14821 acattcatga taaaaatatt tattggtcat tatattaacg tattttatag cactgccgat
    14881 atcacgctca aaaaacaacc actgctattt ttagcaaagc ttatggtata ctccgccgcc
    14941 ttaacatttt tcaccgcaaa ttttcattgc aacatgacga ggaaaataaa tgagtacgcc
    15001 tgatttttct actgccgaga ataatcaaga actggcaaat gaagtctcct gcctgaaagc
    15061 gatgctgacg ctgatgctgc aggcgatggg acaagctgac gcgggccgcg tgatgttaaa
    15121 gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag gctgcagtat tttccaaaac
    15181 ggttaagcaa attaaacaag cctaccgtca gtaatataaa accggctgag agcgtgcctt
    15241 tcagccggtt tttgcatctg gcacgcagaa agaaaagcga tcaaattaag ccagtagcgg
    15301 ccgcgtaaca ggccacctgg gtcttatttg gtgcattgat ttttttctgc atgtttttct
    15361 gatggaaatt gaccgtattc tcagagattg acaaaatcat cgctatctct gctgatgtct
    15421 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15481 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15541 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15601 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15661 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15721 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15781 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15841 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15901 gagcctgaag ctctatttca tggtagacct cttctgcggc ctccatcctc tgaaaacgca
    15961 acagcatcgt gcgacgccaa ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    16021 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    16081 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    16141 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    16201 acaggtgaat cgtcggggct gattttttct tattattgca gcagaaactt ctcgaggaac
    16261 tgtcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    16321 atccgcccca ggtccataaa aatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    16381 tgcgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggattgt gttcaggact
    16441 tcgcccacca gctctggatc cagcgctgac gttggctcgt caaacaaaat cacctcagga
    16501 cgcattgcca gcgcacgcgc aatcgctaca cgctgctgtt gaccgccaga caaacgacgt
    16561 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16621 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16681 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16741 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16801 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctgcgtag caacgtggtt
    16861 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16921 ataccgtgca gcaccgtctg accttggaat tttttcacca ggttcttaac ttcaatggca
    16981 ctcatttggg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    17041 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    17101 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    17161 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    17221 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    17281 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    17341 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacagaatg ggatctaatt
    17401 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    17461 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17521 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17581 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17641 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17701 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17761 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17821 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17881 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17941 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    18001 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccaatc cggcgtgagt
    18061 tccggcactg cccgatgcga ccaccaccga cgaaatatta accgcccctt cacactgttg
    18121 cgcgatttcc agcgcactct ccacataacc cagcgcgccc agagcattag aaccgccaac
    18181 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagct cttccagttg
    18241 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gattcagcaa
    18301 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    18361 cgccacgcag tgcaggccga gtttcgccgc aactgcggca gtctgccgca catggttaga
    18421 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18481 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggtgtgacgt catcccgttt
    18541 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18601 cgcgccgata aactccagcc gtggaaaacg ggttaaatta tgcagtggca taatagcctc
    18661 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18721 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18781 catttttcgg aaagggcttg cagagtgcca tctttttgca tttccgcaat tgcatcgttc
    18841 actgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18901 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18961 agacggtcaa cgaggatcgc atcgatacgg cctacgcgca gatcctgata tttggtcggg
    19021 tcatcatcat aggtacgcac atcgacgccc tgaacattct gccgcagcca ctcttcatag
    19081 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    19141 ccttcgttac cttttttcac cagcgcctga ataccagaaa tggtgtacgg ggtagagaaa
    19201 tcgtattttt tcttgcgctc atcagaaatg gtgacctgat taatcaccac atcaatacgt
    19261 ttagagtcca gcgacgctag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    19321 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    19381 tccccctgaa aactgaacgg cggataagtt ccttccagcc ctaccagtag cgtaccgcgc
    19441 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19501 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19561 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19621 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19681 tgcacaagtc cggtacacgt ttgacgctga tagtgttgat acttccgtaa tgcgatgcgg
    19741 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19801 tgcagctgat cgagggaaat attttgctca tgtagatgat tccccaaacg gcgcaaacga
    19861 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaattgctta
    19921 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19981 cacaatgccg cccatgactg ttgttccact tgccagccat tttcatcaag cagcgtgtcc
    20041 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    20101 agtaatttac ggcaatgcgc gcaatgggcc tggctgtgtt taaagtcttt aagatagcgg
    20161 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    20221 aagtgcggca tttactgacg ttataactta cccagtttag tgcttaaccg tttaatggcc
    20281 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    20341 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    20401 tcgatagctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    20461 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc gcgccactca
    20521 tcgtaggaga aaagctggct gttattggtg tcgagcaaca tttggcgata atcggcaata
    20581 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20641 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20701 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctgcactgc gtaagttgta
    20761 aatgccgttc cttgtagggc gtcatagcgt tcgacggcat taagtaaccc aatgccgccc
    20821 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20881 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20941 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    21001 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    21061 gttaccggta aattccatgc agaaaaaacc ccgccggtgg cggggaagca cgttgctgac
    21121 aaattgcgct ttatgttgcc ggatgcggcg taaacgcctt atccggccta caaaaatgtg
    21181 caaattcaat aaattgcaat tcaccttgta ggcctgataa gtgcagcgca tcaggcaatt
    21241 tggcgttgcc gtcagtctca gttaatcagg ttacgacgat taaccctgca gcagagacag
    21301 aacctgctgc ggtacctggt tggcttttgc cagcacggag ttaccggcct gctggatgat
    21361 ctgcgctttc gacatgttgg acacttcggt cgcatagtcg gcgtcctgaa tacgggactg
    21421 cgcttcagac aggttggtag tggtgttgtt caggttggtg actgcagaat ccagacggtt
    21481 ctgaatagca cccagggatg aacggaattt gtcgatggag ctgatagctt catccagggc
    21541 agccagagga tctttggttg cagtgcctgc actggtggtt tcagtggtca gtttgcctgt
    21601 agagttaaca taagcctgtg ccccaacagt agctgcaaat ttaccagttg cacctgcgcc
    21661 atttgaagct acagttactg taccggtatc cttatcgacg ttgtagttga tagtgtaaga
    21721 tgcggttgtg gtgagctctt tgtctgcatc agtataggtg tcattcgaat taccagcacc
    21781 attagtattc gcaccaccga aggacgccgt tgcagactga atacctgtat tatacttaat
    21841 tgtcgcacca gtaactgctg tgttagccgt tgcagcactc tgcactgtgc tcagtacagt
    21901 atcagcgctt acagttttgg tgtaggttac gccagaagtt gccgctgcac caaagttata
    21961 agttgtgcca gacaactgga tagaaccagt tgtcgctaag gtactaccgc tagcaaacag
    22021 atcggacaat ttcgctgaag atgcaatgcc ggcgttgttt gtagataggt taccagtggc
    22081 gtccaggtac gcggctttac caccgatggt cacatcgcca ttagcatcta catcaaactt
    22141 cacatcacca gtactacgag tataaacacc tgatgcagta gtgccagccg ctggtttcag
    22201 agtattcgca aagttaacaa catcgccatc tgcaactgtt gcttgagtgg tgaagttccc
    22261 tttagccgca tcataagtat agatcgcagc actcgagcca gtagtagtaa ctgtatctcc
    22321 ggttttcagg cgagacagtg catcgctagc gctgagtgct gtattgtttg tggtcacagc
    22381 ataaggacct gttcccgttg caccagcagc ggtcagatcg ctgactgtag cagctttgtt
    22441 cgcaatagtg cctttgccat tcacgttaaa accattcagc cccagcgtat ctgagtcaat
    22501 tttcttcaga tcaatcgtga tagtctggcc gtcattcgca ccaacctgaa ttttcattga
    22561 accgtctttc gccagtacgt tcacgccgtt gaactgggtc tggccagata cgcggtcaat
    22621 ttcgtccaga cgggatttga tttcgtcctg aatggagtcc agatccgaat cggagttagt
    22681 cccggtagaa gcctgaaccg tcagttcaag aatacgctgt aagttgttgt tgatttcgga
    22741 cagcgcgcct tcagtggtct gcgcaacgga gataccgtcg ttggcgttac gggccgcctg
    22801 agtcaggcct ttaatattag aagtaaaacg gttagcaatc gcctgacctg cggcgtcatc
    22861 cttcgcgctg ttaatacgca agccagaaga cagacgctcg atagaactcg acagcgcaga
    22921 ctggttcttg ttgatattat tttgagtgat cagcgagagg ctgttggtat taatgacttg
    22981 tgccatgatt cgttatccta tattgcaagt cgttgattac gtattgggtt tccacccgtc
    23041 ggctcaatcg ccgtcaaccc tgttatcgtc tgtcgtaaaa caacctttag aatttttttc
    23101 acaaacagcc attttttgtt agtcgccgaa atactctttt ctctgcccct tattcccgct
    23161 attaaaaaaa acaattaaac gtaaactttg cgcaattcag gccaataacc ccggtattcg
    23221 ttttacgtgt cgaaagataa aaggaaatcg catggcaagt atttcatcgc tgggagtcgg
    23281 gtcaggtctg gatttaagtt caatccttga tagcctcacc gccgcgcaaa aagcgacgct
    23341 aacccctatt tcaaatcagc aatcgtcgtt taccgctaaa cttagcgcct acggtacgct
    23401 gaaaagcgcg ctgacgactt tccagaccgc caatactgca ttgtctaaag ccgatctttt
    23461 ttccgccacc agcactacca gcagcaccac cgcgttcagt gccaccactg cgggtaacgc
    23521 catcgccggg aaatacacca tcagcgtcac ccatctggcg caggcgcaaa ccctgaccac
    23581 gcgcaccacc agagatgata cgaaaacggc gatcgccacc agcgacagca aactcaccat
    23641 tcaacaaggc ggcgacaaag atccgattac cattgatatc agcgcggcta actcatcgtt
    23701 aagcgggatc cgtgatgcta tcaacaacgc aaaagcgggc gtaagtgcga gcatcattaa
    23761 cgtgggtaac ggtgaatatc gtctgtcagt cacatcaaat gacaccggcc ttgataatgc
    23821 gatgacactc tcggtcagcg gtgatgatgc gctacaaagt tttatgggct atgacgccag
    23881 tgccagcagc aacggtatgg aggtctcggt cgccgccaag aatgcgcagc tgacggtcaa
    23941 caacgtcgcc attgagaaca gcagcaacac catcagcgac gcgctggaaa acatcaccct
    24001 gaacctgaac gatgtcacca cgggcaacca gacgctaacc atcactcagg acacctccaa
    24061 agcgcaaacg gcgattaaag actgggtgaa tgcctataac tcgttaatag ataccttcag
    24121 tagcctgacc aaatacaccg ccgtagatgc gggagctgat agccagagtt ctagcaatgg
    24181 cgcactgctc ggcgactcca cgctgcggac gattcagacg cagttgaagt cgatgctgag
    24241 taataccgtc agttcttcca gctataaaac gctggcgcag attggtatca cgaccgatcc
    24301 cagcgatggc aaactggaac tggatgccga caaactcacc gctgcactga aaaaagatgc
    24361 cagcggcgta ggtgcattga ttgttggcga tggtaaaaaa accggcatca cgaccaccat
    24421 cggcagcaac ctgaccagtt ggctttcgac aacgggcatt attaaagccg ctaccgatgg
    24481 cgttagtaag accctgaata agttaactaa agactacaac gccgccagcg atcgcattga
    24541 tgcgcaggtc gcgcgctaca aaaaacaatt tacccaactg gacgttttaa tgacctcgtt
    24601 aaacagcacc agcagctact taacgcagca gttcgaaaac aacagtaatt ccaagtaatc
    24661 attattcatc gggagacagg tcatgtacgc ggcaaaaggc acccaggcct atgcacaaat
    24721 tggcgtcgaa agcgccgtaa tgagcgccag ccagcagcag ctggtcacca tgctatttga
    24781 tggagtgctg agcgcactgg ttagagcgcg cctgtttatg caggacaaca atcagcaagg
    24841 caaaggcgtc tctttgtcaa aagcgatcaa catcattgag aacggactgc gggtgagtct
    24901 tgatgaagag agcaaagacg aactaaccca aaacttgatt gctctttata gctatatggt
    24961 caggcgcttg ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag aagtggaagc
    25021 attaatgcgc aatattgccg atgcctggaa agagtcgtta ctctcccctt ctttgattca
    25081 ggacccagtc tgatgaacca tgcaccgcat ttgtatttcg cctggcaaca actcgtcgaa
    25141 aaaagccagc tcatgttacg cctggcaacg gaagaacaat gggacgaact catcgccagc
    25201 gaaatggcgt atgtgaatgc ggtgcaggag tatagcacac ctgactgaag aggttgcccc
    25261 gtccaccacg atgcaggagc agctccgccc gatgctgcgc ctgattctcg acaacgaaag
    25321 caaggtaaag cagttattac agattcgtat ggatgaactg gcgaaactgg tcggtcagtc
    25381 atcggtgcaa aaatcggtgt taagtgccta tggcgatcag ggcggctttg tgctggctcc
    25441 gcaagataac ttctcgtgaa tcttaacgag ctaatggttc gcgaataatc cgattacggc
    25501 tacgcttcta atgttcccct tgaatggagt cgaagaatgc gtaatcccac gctgttacaa
    25561 tgttttcact ggtattaccc ggaaggcggt aagctctggc ctgaactggc cgagcgcgcc
    25621 gacgttttta atgatattgg tatcaatatg gtctggttgc cgcccgccta taaaggcgca
    25681 tcgggcgggt attcggtcgg ctacgactcc tataatttat ttgatttagg cgagtttgat
    25741 cagaaaggca gcatccctac taaatatggc gataaagtac aattgctggc cgccattgac
    25801 gccctgaaac gtaatgacat tgcggtgctg ttggacgtgg tagtcaacca caaaatgggc
    25861 gcggatgaaa aagaagctat tcgcgtgcag cgtgtaaatg ctgacgaccg tacgcaaatt
    25921 gatgaagaaa tcattgagtg cgaaggctgg acgcgttaca ccttccccgc ccgtgccggg
    25981 caatactcac agtttatctg ggatttcaaa tgttttagcg gtatcgacca tatcgaaaac
    26041 cctgatgaag atggcatatt taaaattgtt aacgactaca ccggcgaagg ctggaacgat
    26101 caggttgatg atgaattagg taatttcgat tatctgatgg gcgagaatat cgattttcgc
    26161 aatcatgccg tgacggaaga gattaaatac tgggcgcgct gggtgatgga acaaacgcaa
    26221 tgcgacggtt ttcgtcttga tgcggtcaaa catattccgg cgtggtttta taaagagtgg
    26281 atcgaacacg tacaggaagt tgcgccaaag ccgctgttta ttgtggcgga gtactggtcg
    26341 catgaagtcg ataaactgca aacgtatatt gatcaggtgg aaggcaaaac catgctgttc
    26401 gatgcgccgc tgcagatgaa attccatgaa gcatcgcgca tggggcgcga ctacgacatg
    26461 acgcagattt tcacgggtac attagtggaa gccgatcctt tccacgccgt gacgctcgtt
    26521 gccaatcacg acacccaacc gttgcaagcc ctcgaagcgc cggtcgaacc gtggtttaaa
    26581 ccgctggcgt atgccttaat tttgttgcgg gaaaatggcg ttccttcggt attctatccg
    26641 gacctctacg gtgcgcatta cgaagatgtc ggtggtgacg ggcaaaccta tccgatagat
    26701 atgccaataa tcgaacagct tgatgagtta attctcgccc gtcagcgttt cgcccacggt
    26761 gtacagacgt tatttttcga ccatccgaac tgcattgcct ttagccgcag tggcaccgac
    26821 gaatatcccg gctgcgtggt ggtcatgtcg aacggggatg atggcgaaaa aaccattcat
    26881 ctgggagaga attacggcaa taaaacctgg cgtgattttc tggggaaccg gcaagagagc
    26941 gtaataaccg acgaacacgg cgaagcaacc ttcttttgca acggcggcag cgtcagcgtg
    27001 tgggttatcg aagaggtgat ttaaattcat ccccggcggc aagccgggga gatttcatta
    27061 cggcagtggc gtcggtaacg gcgctttatc cagcgcggcg gcacactctg ccgtcgggcg
    27121 atccacacgc tccatcgtca taccatcgta ctcaatggtg ttaccgtcac gctcaacctc
    27181 gtacagctca cgttttacgg taacgttagt gagatcatca gacatcaatg ccaacttacc
    27241 cggcacagca atcacccgct gccactgacg gcaatccaga gtatcaccct ctttagtgac
    27301 aatcaggctg cctatcgctt ccggactgac cagcgcacgt tgcggccctt tggtctgcca
    27361 gtagcccgcc agccagtccg gcgcgggggt tttcacgaca ttgttatagt tttcgacttc
    27421 ggcacagccc gccagcaaca tgagcgcgcc tgcaattgcg agttttttca tcattcgtcc
    27481 tgcatgagaa gaaaagagga ttgtggcatt aaagccctga tgtcgccagc ctttccaggt
    27541 tcagagagaa atttgatcta actattattt ttgtgaatta ttaccccgaa aataaatttg
    27601 catgatgtat gattcgcgac tcttgtttca aaccttctga gttcagaggc tacattcatg
    27661 tcatggcagc aattcaaaca cgcctggttg attaaattct gggcacccat ccctgcggtc
    27721 atcgcggcgg gtattctctc cacttactat tttggcatta ctggcacctt ttgggctgtc
    27781 acgggtgaat ttacccgttg gggcggccag ctcctgcagc tgtttggcgt ccatgctgaa
    27841 gagtggggtt attttaaaat tatccatctg gaaggatcgc cattaacccg catcgacggg
    27901 atgatgatcc tcggcatgtt tggcggctgc tttgccgcag cgctgtgggc caacaatgtc
    27961 aaactgcgca tgccacgcag ccgtatccgc attatgcagg ccattattgg cggtattatc
    28021 gctggttttg gcgcgcgtct ggcaatgggc tgtaacctgg cggcgttctt taccggtatt
    28081 cctcagttct cgctgcatgc ctggttcttt gccatcgcca ctgccattgg ttcatggttt
    28141 ggcgcacgct ttaccctgct gcccatcttc cgtattcccg tgaaaatgca gaaagtttct
    28201 gccgcctcac cgctgacgca aaaaccggat caggcgcggc gtcgttttcg tctcgggatg
    28261 ctggtctttt tcggtatgct gggctgggcg ctgctcacgg cgatgaacca accaaaactg
    28321 gggctggcaa tgctgtttgg cgtcggcttt ggtttactga ttgaacgtgc gcaaatctgc
    28381 tttacttcgg cgttccgcga tatgtggatc accggacgta cccatatggc gaaagcaatc
    28441 attatcggta tggcggtgag tgccatcggg atcttcagtt acgtacagtt aggcgttgaa
    28501 cccaaaatca tgtgggcggg accaaacgcg gtaattggtg gtttactgtt tggttttggc
    28561 atcgtgctgg caggcggctg cgaaaccggc tggatgtacc gcgcggtaga aggccaggtg
    28621 cactactggt gggtcggtct gggcaatgtg atcggctcga cgattctggc gtattactgg
    28681 gatgatttcg ctccggcgct ggccaccgac tgggacaaaa tcaacctgct gaaaaccttt
    28741 ggcccgatgg gtggcctgct ggtgacatat ttgctgttgt ttactgcgct gatgttgatt
    28801 atcggctggg aaaaacgctt cttccgccgt gcggcaccgc agactgctaa ggagatcgca
    28861 tgaaaaatat cgttcctgat taccgccttg atatggtggg tgaaccctgc ccttatccgg
    28921 cagtcgcaac ccttgaggcg atgccgcagt taaaaaaagg ggaaatcctg gaagtggtga
    28981 gcgactgtcc acagtcgatc aataatattc cactggatgc gcgtaatcac ggctatacgg
    29041 tgcgggatat tcagcaagac gggccgacca ttcgttattt aattcagaag taatccctca
    29101 ttcccgctgt ggcccacagc gggaacatcg ttgcctctga cacatagatt ttacccgccc
    29161 cctctcctat tcttttcagt atgatctcag gaggtttcta tgtgtggacg ctttgcccaa
    29221 tcccaaacgc gtgaagatta ccttgcgctt ctcgcggaag atattgaacg cgatattccc
    29281 tacgatcccg aacccattgg cagatacaac gtcgcgccgg gaaccaaagt tcttctgctc
    29341 agtgaacgtg atgaacacct tcatctggat ccggttttct ggggatatgc tcccggatgg
    29401 tgggataaac cgccgctgat taacgcccgc gtagaaactg ccgcaaccag tcgcatgttt
    29461 aaaccgctct ggcaacatgg tcgggcaatc tgttttgccg atggctggtt tgagtggaaa
    29521 aaagaaggcg acaaaaaaca gccttttttt atctatcgtg ctgatggaca acctattttt
    29581 atggccgcga taggtagcac accatttgag catggtgacg aagccgaagg atttttaatt
    29641 gtcactgctg cagcagacca gggtctggta gacattcatg accgccgccc gctggttctg
    29701 tcgccagaag ctgcacagga atggatgcgg caagagattg gcggtaaaga agcatcagaa
    29761 attgctacca acggctgtgt gccagcaaac cagttcacct ggcacccggt atcgcgcgcg
    29821 gtaggtaatg tcaaaaacca gggcgcggag ttaattcaac ctgtttgttg acatcctcca
    29881 cgccctgaag gacgaggctc cctgctacgt tcaggctgtc gcctgaatca tttcggtggg
    29941 ttcctgcttc aacgggcggc ctgactgcac catctctcca caggcaagaa cggcgtgccc
    30001 cgccgctaaa atgttacgag cgccatttac atcggcgttc gctgtatatc cacatacctg
    30061 gcatctgaat ttactttgtg acaggcggtt ttctttcgct gtatgaccac agcacgcgca
    30121 acgctggctt gtgcacgctg gcggaacagc aaacacctga ccgccacgcc agagctgctt
    30181 gtactc
//
LOCUS       DAJTOR010000032        29250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTOR010000032 DAJTOR010000000
VERSION     DAJTOR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.032"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..145
                     /locus_tag="OO249_001282"
     CDS             <1..145
                     /locus_tag="OO249_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HCR5635643.1"
                     /translation="GGRSETELTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKS
                     FDW"
     gene            420..3269
                     /gene="rcsC"
                     /locus_tag="OO249_001283"
     CDS             420..3269
                     /gene="rcsC"
                     /locus_tag="OO249_001283"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5635644.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGGEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGEPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3469..4119)
                     /gene="rcsB"
                     /locus_tag="OO249_001284"
     CDS             complement(3469..4119)
                     /gene="rcsB"
                     /locus_tag="OO249_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5635645.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4136..6808)
                     /gene="rcsD"
                     /locus_tag="OO249_001285"
     CDS             complement(4136..6808)
                     /gene="rcsD"
                     /locus_tag="OO249_001285"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5635646.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTISDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7547..8677
                     /gene="ompC"
                     /locus_tag="OO249_001286"
     CDS             7547..8677
                     /gene="ompC"
                     /locus_tag="OO249_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5635647.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINSRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8789..9844
                     /gene="apbE"
                     /locus_tag="OO249_001287"
     CDS             8789..9844
                     /gene="apbE"
                     /locus_tag="OO249_001287"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5635648.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GINGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9918..10970
                     /gene="ada"
                     /locus_tag="OO249_001288"
     CDS             9918..10970
                     /gene="ada"
                     /locus_tag="OO249_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5635649.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCPVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVLANAIGKPKAVRAVASACAANKLAIVIPCHRVVGGDGTLSGYRWGV
                     SRKAQLLRREAENEER"
     gene            10970..11620
                     /gene="alkB"
                     /locus_tag="OO249_001289"
     CDS             10970..11620
                     /gene="alkB"
                     /locus_tag="OO249_001289"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5635650.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTIHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFYPLTTDCRYNLTFRQAGKKE"
     gene            11696..13339
                     /gene="yojI"
                     /locus_tag="OO249_001290"
     CDS             11696..13339
                     /gene="yojI"
                     /locus_tag="OO249_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5635651.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLMAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13557..15203
                     /gene="mqo"
                     /locus_tag="OO249_001291"
     CDS             13557..15203
                     /gene="mqo"
                     /locus_tag="OO249_001291"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR5635652.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(15353..15841)
                     /gene="eco"
                     /locus_tag="OO249_001292"
     CDS             complement(15353..15841)
                     /gene="eco"
                     /locus_tag="OO249_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5635653.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(15976..16140)
                     /locus_tag="OO249_001293"
     CDS             complement(15976..16140)
                     /locus_tag="OO249_001293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635654.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            16249..16743
                     /gene="napF"
                     /locus_tag="OO249_001294"
     CDS             16249..16743
                     /gene="napF"
                     /locus_tag="OO249_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5635655.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            16733..16996
                     /gene="napD"
                     /locus_tag="OO249_001295"
     CDS             16733..16996
                     /gene="napD"
                     /locus_tag="OO249_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5635656.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            16993..19479
                     /gene="napA"
                     /locus_tag="OO249_001296"
     CDS             16993..19479
                     /gene="napA"
                     /locus_tag="OO249_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5635657.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTLITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTQDATDPLSKETDFKKCAVKLEKV"
     gene            19486..20181
                     /gene="napG"
                     /locus_tag="OO249_001297"
     CDS             19486..20181
                     /gene="napG"
                     /locus_tag="OO249_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5635658.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            20168..21031
                     /gene="napH"
                     /locus_tag="OO249_001298"
     CDS             20168..21031
                     /gene="napH"
                     /locus_tag="OO249_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5635659.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            21028..21477
                     /gene="napB"
                     /locus_tag="OO249_001299"
     CDS             21028..21477
                     /gene="napB"
                     /locus_tag="OO249_001299"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5635660.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            21487..22089
                     /gene="napC"
                     /locus_tag="OO249_001300"
     CDS             21487..22089
                     /gene="napC"
                     /locus_tag="OO249_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5635661.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            22108..22725
                     /gene="ccmA"
                     /locus_tag="OO249_001301"
     CDS             22108..22725
                     /gene="ccmA"
                     /locus_tag="OO249_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5635662.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            22722..23384
                     /gene="ccmB"
                     /locus_tag="OO249_001302"
     CDS             22722..23384
                     /gene="ccmB"
                     /locus_tag="OO249_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5635663.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            23426..24163
                     /gene="ccmC"
                     /locus_tag="OO249_001303"
     CDS             23426..24163
                     /gene="ccmC"
                     /locus_tag="OO249_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5635664.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            24160..24369
                     /gene="ccmD"
                     /locus_tag="OO249_001304"
     CDS             24160..24369
                     /gene="ccmD"
                     /locus_tag="OO249_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5635665.1"
                     /translation="MTLAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            24366..24845
                     /gene="ccmE"
                     /locus_tag="OO249_001305"
     CDS             24366..24845
                     /gene="ccmE"
                     /locus_tag="OO249_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5635666.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24842..26791
                     /gene="ccmF"
                     /locus_tag="OO249_001306"
     CDS             24842..26791
                     /gene="ccmF"
                     /locus_tag="OO249_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5635667.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     MLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIAIASLLSGRLDSTYARFTRPWTLAAW
                     IFLTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKA
                     WTLLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAA
                     RGHKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPF
                     FNTMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKV
                     VAMTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQN
                     YSVERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEK
                     RYYNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMAL
                     GGLLCLFDPRYRKRVSPQKTAPEAV"
     gene            26788..27345
                     /gene="dsbE"
                     /locus_tag="OO249_001307"
     CDS             26788..27345
                     /gene="dsbE"
                     /locus_tag="OO249_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5635668.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            27342..28394
                     /gene="ccmH"
                     /locus_tag="OO249_001308"
     CDS             27342..28394
                     /gene="ccmH"
                     /locus_tag="OO249_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5635669.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(28429..29076)
                     /gene="narP"
                     /locus_tag="OO249_001309"
     CDS             complement(28429..29076)
                     /gene="narP"
                     /locus_tag="OO249_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5635670.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPSFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         6678 a         7219 c         8105 g         7248 t
ORIGIN      
        1 tggtgggcgc agcgaaacgg aattaaccac cgcccaactg acgctgatca ccgatgaagg
       61 atcggtcaat gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac
      121 gctggtgaaa agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt
      181 cagataagac tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca
      241 gattggtcat ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt
      301 aaggcaatgg tttctaagcg atacgcatta ttgcaataat gacagaacat tgcggtaaca
      361 cgcttttacc gctaccttaa ccacactcca tcggtcacct gaggcggagc ttcgcccctt
      421 tgaaatacct tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat
      481 tggcgttagt gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt
      541 tacatcagcg agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc
      601 gctttattca acgcacctct gatgtgatga aagagctgaa gtacatcgcc gaaaatcgct
      661 tatcggcaga aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg
      721 cgtttgaacc gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt
      781 ctctggagtc attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg
      841 atctaaaccg ggtattttta atcggcagcg ataacctctg catggccaat ttcggtctgc
      901 gtgatatgcc agtggaacgc gataccgcgt tgaaagcttt gcatgaacgc atcaataaat
      961 atcgaaatgc accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc
     1021 gccctggcgt cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg
     1081 cgcttttggg gggcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc
     1141 cgatgggggt taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag
     1201 aaagtaaaat taagggcgag cctcgctgga tgcaggaacg ctcctggttt ggctatacgg
     1261 aagggttccg ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt
     1321 attcggtgcc ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt
     1381 tgctgaatgt gcttgccgga gctgcattgt ttactctcgc acggatgtac gagcgacgta
     1441 ttttcattcc ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca
     1501 agattgtcgc ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata
     1561 ttttaagtaa cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac
     1621 gactgacgca aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca
     1681 ataccaatct gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt
     1741 gtgtgctggt ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac
     1801 aagcagcgga acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc
     1861 tgcgaacgcc gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac
     1921 cgaaaggcgt cgatcggctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa
     1981 ttatcagcga tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc
     2041 gtgagttttc accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg
     2101 tacgcaagca gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg
     2161 gcgacccgat gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca
     2221 ccgataccgg ctgtatagtt ttgcatgtcc gcgctgatgg cgattatctc tctatccgtg
     2281 ttcgcgatac cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct
     2341 tccaggtcgg aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt
     2401 gtgaaaaact gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg
     2461 gcagccagtt taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg
     2521 tggaagggtt gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt
     2581 tcctagaaac tagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac
     2641 cgactcccga agatgtgtta attactgacg aggtagtgag taaaaaatgg cagggtagag
     2701 cggtagtgac cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt
     2761 gggtacacag tgtggctgcc ccgcatgagc taccggcatt gttggcgcgt atttatttga
     2821 tcgagatgga gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg
     2881 ataatgacga tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg
     2941 cagatcagtt gggatcgttg ggctatcaat gtaaaaccgc gaatgatggt gtcgatgcgc
     3001 ttaatgtact tagcaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata
     3061 tggatggtta ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg
     3121 gagtaactgc taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca
     3181 gctgcctgtc gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg
     3241 agagggtcag gaaatcgcgg gaatcgtagg acggataagg tgtttatgtc gcatctggca
     3301 ttcagtgcaa atgccagatg cgacgctgac gcgtcttatc tggcctactt tgatgtaatc
     3361 tgttgaaata atgggaatcg taggacgcat aaggcgttta cgccgcatcc ggcaatcggt
     3421 gcaaatgcta gataagacac taacgcgtct tatctggcct acaggtgatt agtctttatc
     3481 tgccggactt aacgtcactg aagagagata attcagcagg gcgatatcgt tctcgacacc
     3541 cagcttcatc atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt
     3601 tttagcgatc tcggtcacca agaagccttc cgcaaacagg cgcagaactt cactctcttt
     3661 tggcgagaga cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac
     3721 gctttccggg gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt
     3781 cggtgcacct tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat
     3841 cgccgggttg ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat
     3901 gtacttgatt aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat
     3961 caacacatgc gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc
     4021 gccgacaaca ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat
     4081 cggatggtca tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca
     4141 gcaagctctt gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct
     4201 tctcacgaat cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca
     4261 tggcaaatac gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg
     4321 ctgcttcagt atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat
     4381 agccgctggc atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt
     4441 cctgcgccag ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat
     4501 tgaagttgac gcacaattga ccaggcccaa tttcccgtac gccagactca tcatcgctta
     4561 aaagcaagcc agaggcagta agattagacg gattatccgt taaaaagata tcataatctt
     4621 gactaattaa tctttcatcg ggtgtgatac aggttgcacc ccaattttct aactggcgag
     4681 tgacaatatt ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta
     4741 aacgctcttc ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt
     4801 agcgtgtacc aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgcgcca
     4861 gttgatcgct cagccagaat gccagcgggt ccgccttgcc atagcgatcg ttttggggct
     4921 ggttgataaa cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt
     4981 ccagaatgcg gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga
     5041 tttttcccaa ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta
     5101 aggcatcgcg atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt
     5161 gcagaccttt acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct
     5221 gcacggagaa cagcaccgtc tcacttttcc agctatcgtc cgcaagcatg ttcgctaact
     5281 gtatttcatc gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg
     5341 gggcgttgag tttagccgcg ctctccgcca gggactgtgc gggttctttc agcgcatcgc
     5401 caatgttttt cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct
     5461 gcttgagttt cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg
     5521 tgcgcggcgc gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg
     5581 cctgaataat cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcaaatgcg
     5641 gcagcaaatg atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa
     5701 ccagcaggcc gagcggcagc agtgagacta tctcttcatt aattgcccgt aaaatgcgca
     5761 attcgttatt gaccgccgtg ctgggtagac tttctgtact gcggctggag aaatggcgga
     5821 atgtggtata gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca
     5881 gaatgttttg caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca
     5941 tatcggtaga gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga
     6001 cgctatccgt cccttctttc tcattatcat tgtctcccgt tgccgtcgcg tctggctcaa
     6061 ggcggaaact gtccagcggc atacccggtg ggatcaaatc attaatcggt agatcaaaag
     6121 ctacgaccgt tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac
     6181 cgttctgcca ggccaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg
     6241 cctgttgcaa catctctgca cgacgtgaat caacaatgtc gctgatggtc gattctttaa
     6301 atccggaggt gagatctttg aggggcaggg ttgagatcag caccagactg ttatcctgac
     6361 cattcaggta atacatcgac cacggtacat tttctgcgcc ccacaatgtg tccagatagg
     6421 tggacatccg ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt
     6481 ccgttttgcg gcgcggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta
     6541 aaccttcgcc ggaggagggg gaggttgtcg cggcaatgtt gtcgtagatc tgccaggtca
     6601 cgtaacgcca ggtattgacg cgcttttgga tagcgtgggt aatgtcgaca atctggtaac
     6661 ttttatcttt cagccatgcg ttaacggcgc tttgtaccat tacacccatc gtcaccagta
     6721 acacaatgat caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg
     6781 tcgtggccgt tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag
     6841 tgatagggca gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc
     6901 tctattctcc cgtgatattt atcatcctgc attgggggaa atagatgtac aagcgccatt
     6961 ttgctgtaca aataacctac aaaaagccca acgaaattaa ttgcaaataa acgatgattg
     7021 ctggaaatta tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct
     7081 gtggtagcac agaataatga aaagtgtgta aagaagggta aaaaaaaccg gatgcgaggc
     7141 atccggttga aataggggta aacagacatt cagaaatgaa tgacggtaat aaataaagtt
     7201 aatgatgata gcgggagtta ttctagttgc gagtgaaggt tttgttttga cattcagtgc
     7261 tgtcaaatac ttaagaataa gttattgatt ttaaccttga attattattg cttgatgtta
     7321 ggtgcttatt tcgccattcc acaataatct taaaaagttc ccttgtattt acattttgaa
     7381 acatctatag cgataaatga aacatcttaa aagttttagt atcatattcg tgttggatta
     7441 ttctgcattt ttggggagaa tggacttgcc gactgattaa tgagggttaa tcagtatgca
     7501 gtggcataaa aaagcaaata aaggcatata acagagggtt aataacatga aagttaaagt
     7561 actgtccctc ctggtcccag ctctgctggt agcaggcgca gcaaacgctg ctgaagttta
     7621 caacaaagac ggcaacaaat tagatctgta cggtaaagta gacggcttgc actatttctc
     7681 tgacaacaag tcagaagacg gcgaccagac ctatgtacgt cttggtttca aaggcgaaac
     7741 tcaggttact gaccagctga ccggttacgg ccagtgggaa tatcagatcc agggcaatac
     7801 ctctgaagac aacaaagaaa actcctggac ccgtgtggca ttcgcaggtc tgaaattcca
     7861 ggatgtaggt tctttcgact acggtcgtaa ctacggcgtt gtttacgacg taacttcctg
     7921 gaccgacgta ctgccagaat tcggtggcga cacctacggt tctgacaact tcatgcagca
     7981 gcgtggtaac ggcttcgcga cctaccgtaa caccgacttc ttcggtctgg ttgacggtct
     8041 gaactttgct gttcagtacc agggcaaaaa cggtagcgta agcggcgaag gcatgaccaa
     8101 caacggtcgt ggcgctctgc gtcagaacgg cgacggtgtc ggcggttcta tcacttatga
     8161 ttacgaaggc ttcggtatcg gtgctgcagt ttccagctcc aaacgtactg atgatcaaaa
     8221 tggtagctac atcagcaatg gtgtagttcg taactacatc ggtactggcg accgtgctga
     8281 aacctacact ggtggtctga aatacgacgc taacaacatc tacctggctg ctcagtacac
     8341 ccagacctac aacgcaactc gcgtaggttc cctgggttgg gcgaacaaag cacagaactt
     8401 cgaagctgtt gctcagtacc agttcgactt cggtctgcgt ccgtctgtag catacctgca
     8461 gtctaaaggt aaaaacctgg gtgtcatcaa tagtcgtaac tacgacgacg aagatatcct
     8521 gaaatatgtt gatgttggcg cgacctacta cttcaacaaa aacatgtcca cctatgttga
     8581 ctacaaaatc aacctgctgg acgacaacca gttcactcgt gacgctggca tcaacactga
     8641 taacatcgta gctctgggtc tggtttacca gttctaatct cgattgatat cgaacaaagg
     8701 gcctgcgggc ccttttttca ttgttttcag cgtacaaact cagttttttg gtgtactctt
     8761 gcgaccgttc gcatgaggat aatcacgtat ggaaataagc tttacccgcg tggcactgct
     8821 ggctgccgcg ctcttctttg ttggttgcga tcaaaaacca caacccgcca aaacccacgc
     8881 tactgaagtt accgttcttg aaggcaaaac catgggtacc ttctggcgtg ccagcatccc
     8941 gggcattgat gccaaacgca gtgccgaact taaagaaaag attcagaccc agctggacgc
     9001 cgacgatcag ctgctttcga cctataaaaa agattccgcg ctgatgcgct ttaacgactc
     9061 gcaaagtttg tcgccgtggc cagtaagtga agcgatggcc gaaatcgtca ctacctcgct
     9121 gcgcattggc gcgaagaccg atggtgcgat ggatattacc gtcgggccgc tggtaaatct
     9181 gtggggcttt ggcccggaac aacagccggt tcaaattccg agccaggaac agatcgacga
     9241 gatgaaagcc aaaactggtt tacagcacct tacggtcatt aatcagtcgc atcagcaata
     9301 tctgcaaaaa gacctgccgg atttatatgt cgatctctct accgtcggcg aaggttatgc
     9361 ggcggatcac ttggcacgct tgatggagca ggaagggatt tcccgctatc tggtgtcggt
     9421 gggcggcgcg ctgaacagcc gtggtatcaa cggtgaaggc ctgccgtggc gggtagcgat
     9481 tcaaaaacca accgataaag aaaacgcagt tcaggctgtg gtggatatca acggtcatgg
     9541 gattagcacc tctggcagct accgtaacta ttacgaactg gacggcaaac gtctttccca
     9601 tgttatcgat ccgcaaaccg ggcgtcccat cgaacacaat ctggtatccg tgacggtgat
     9661 tgctccgacg gcgctggaag ccgatgcctg ggatactggc ttgatggtac tcgggccgga
     9721 gaaagccaaa gaggttgttc gccgggaagg gctggcggtt tatatgatca ccaaagaagg
     9781 cgatagcttt aaaacctgga tgtcaccaca gtttaaaagc ttccttgtca gcgaaaaaaa
     9841 ttaaagcgca agattgttgg tttttgcgtg atggtgaccg ggcagactaa aggctatcct
     9901 taaccaggga gctgattatg aaaaacgcca catgcttaac tgacgatcaa cgctggcaat
     9961 ctgtcttagc ccgcgacccg aatgccgacg gcgaattcgt tttcgccgtg cgcaccacag
    10021 gcatcttttg ccgtccgtct tgccgcgcca gacatgcctt gcgggaaaat gtctccttct
    10081 acgcaaatgc cagcgaggca ctcgccgccg gatttcgccc ctgcaaacgc tgccaacctg
    10141 ataaagccaa tgcccagcaa catcggttgg ataaaatcac ccacgcgtgt cgactgctgg
    10201 aacaggaaac gcctgtaacg ttggaagcct tagccgacca ggtggcgatg agtccatttc
    10261 atctacatcg gttgtttaaa gcgactaccg gaatgacgcc taaagcctgg caacaggcct
    10321 ggcgcgctcg ccgtttgcgc gaatcgctgg cgaaagggga gagcgtgacg acgtctattc
    10381 ttaacgccgg attccccgac agcagcagtt actaccgcaa agccgatgaa acgctgggca
    10441 tgacggctaa acaattccgt cacggtggcg aaaatctggc ggtgcgttac gcgctggctg
    10501 attgtgcgct gggtcgttgc ccggtggcag aaagcgagcg ggggatttgc gcgatattgc
    10561 tgggcgatga tgacgcgaca ttaatcagcg agttgcagca gatgtttccc gctgccgaca
    10621 acgcgcctgc cgatctgacg tttcagcaac atgtgcgtga agtgatcgcc agcctcaatc
    10681 aacgcgatac gccgctgacg ttaccgctgg atattcgcgg cactgctttt cagcaacaag
    10741 tctggcaggc actgcgcacg ataccttgcg gtgaaaccgt actggctaac gccatcggca
    10801 aaccgaaagc ggtacgggcc gttgccagcg cctgtgccgc caacaagctg gctatcgtaa
    10861 taccctgtca tcgagtggtc ggtggtgatg gcacactttc cggttaccgc tggggcgtgt
    10921 ctcgtaaagc gcaactgctg cgccgcgaag ctgaaaatga ggagaggtaa tgttggatct
    10981 gtttgccgat gctgaaccgt ggcaagagcc actggcggct ggtgcggtaa ttttacggcg
    11041 ttttgctttt aacgctgcgg agcaactgat ccgcgatatt aatgacgttg ccagccagtc
    11101 gccgtttcgc cagatggtca cccccggggg atataccatg tcggtggcga tgaccaactg
    11161 tgggcatctg ggctggacga tccatcggca aggttatctc tattcgccca ttgatccgca
    11221 aacaaataaa ccgtggcccg ccatgccaca aagctttcat gatttatgtc aacgtgcggc
    11281 tacggcggcg ggctatccag atttccagcc agatgcttgt cttatcaacc gctacgctcc
    11341 tggcgcgaaa ctgtcgctgc atcaggataa agacgaaccg gatctgcgcg cgccaattgt
    11401 ttctgtttct ctgggcttac ccgcgatttt tcaatttggt ggcctgaaac gtaatgatcc
    11461 gctcaaacgt ttgttgttgg aacatggcga tgtggtggta tggggcggtg aatcgcggct
    11521 gttttatcac ggtattcaac cgttgaaagc ggggttttat ccactcacca ccgactgccg
    11581 ctacaacctg acattccgtc aggcaggtaa aaaagaataa aaataagaat tattattgct
    11641 gtgcgcgcga agatgtttaa actgcgggct gtaattcatt gtccgggttt tctgcatgga
    11701 acttcttgta cttgtctggc ggcagtatcg ctggccattt atcagtgtga tggcgctaag
    11761 cctcgccagt gcggcattag gcattggctt aattgctttt atcaatcagc gccttatcga
    11821 aatggcggat accagtctgc tggtattgcc ggagtttctg ggattattgc tgctgttgat
    11881 ggcagtcact ctcggatcgc aactggcgct caccactttg gggcatcact tcgtttaccg
    11941 actgcgtagt gaatttatca agcggattct ggatacccac gtcgagcgca ttgaacaact
    12001 cggtagcgcc tcgttgttgg cgggattaac cagcgatgtg cgcaatatca ccattgcttt
    12061 tgtgcgtctt ccggaactgg tgcaggggat cattctcact atcggttccg cggcgtatct
    12121 gtggatgctg tcgggcaaaa tgttgctggt aacggctatc tggatggcga tcaccatctg
    12181 gggtggtttt gtactggtgg cgcgggtgta caaacatatg gcgaccctac gtgaaaccga
    12241 agacaagctg tacacggatt ttcaaactgt acttgaaggg cgcaaagagc tgactctgaa
    12301 ccgggaacgc gccgagtatg tgtttaacaa cctctacatc cccgatgcgc aagagtatcg
    12361 ccatcatatt attcgcgcag acaccttcca tcttagtgcc gtgaactggt caaacatcat
    12421 gatgctgggc gctatcggcc tggtgttctg gatggcgaac agtctcggtt gggctgatac
    12481 caacgttgct gcgacctatt cgttgacgct tttattcctg cgtacgccgc tgctttcggc
    12541 ggttggcgca ttgccgacgc tgctgatggc gcaggtggcg tttaacaagc tgaacaaatt
    12601 cgcgctcgcg cctttcaaag cagagtttcc gcgcccgcag gcgtttccca actggcaaac
    12661 gctggagctg cgtaatgtga cgtttagcta tcaggataac gcgttttccg ttggtccgat
    12721 taatctcacc atcaaacgtg gcgagctact gtttctgatt ggtggtaacg gtagtggaaa
    12781 atcgacgctg gcgatgttgt tgacgggctt gtatcagcca caaagcggcg aaatcttgct
    12841 ggatggcaaa cctgtcagcg gcgaacaacc ggaagattat cgcaaactgt tttcggcagt
    12901 gtttaccgat gtctggcttt ttgatcaact gctggggccg gagggtaaac ccgctaaccc
    12961 gcaactggtt gagaagtggc tggcgcagct gaaaatggct cataagcttg agttaagcaa
    13021 cgggcgtatt gttaacctga agttatcaaa agggcagaaa aaacgcgtgg cgctgttgct
    13081 ggcgctggca gaagaacgcg atattatcct gctggatgaa tgggcggcgg atcaggatcc
    13141 acacttccgt cgtgagtttt atcaggtgtt gctgccgctg atgcaggaga tgggtaaaac
    13201 tattttcgct atcagtcatg atgatcatta ctttatccac gccgaccgcc tgctggaaat
    13261 gcgcaatggg caacttagcg agctgacggg cgaagagcgc gatgccgctt cgcgtgatgc
    13321 cgttgcccgg acggcataat attacgacgc tgacctggcg gactgctgcc gtcaggtcaa
    13381 tatgctaaaa atcccttcat ttctaataac cctataatta atcaacgaaa ttataatgtt
    13441 tctaaaatta gaatataatt tataaacatt atttaaatgt tgttacttaa gtgttacctt
    13501 tgatgccgcg caaatctcac ctgcaataaa gcgactaaaa gtaaggcatt aacaagatga
    13561 aaaaagtgac tgccatgctc ttctcgatgg ccgtggggct taatgccgtt tcgatggcgg
    13621 caaaagcgaa agcgtccgag gagcaggaaa ctgatgtact gttgattggc ggcggcatta
    13681 tgagcgccac gttggggacc tatttacgcg agctggagcc tgaatggtcg atgaccatgc
    13741 tggagcgcct ggagggtgtc gcgcaggaga gttcgaacgg ctggaataac gccggaaccg
    13801 ggcattctgc actgatggaa ctgaactaca ccccgcaaaa cgccgatggc agtatcagta
    13861 ttgaaaaagc agtcgccatt aacgaagcat ttcagatttc ccgccagttc tgggcgcacc
    13921 aggtcgagcg cggcgtgctg cgtactccgc gttcatttat caataccgtt ccgcatatga
    13981 gctttgtctg gggcgaggat aacgttaatt tcctgcgcgc ccgttacgcc gcgttgcaac
    14041 aaagctcgct gtttcgcggt atgcgttact ctgaagatca cgcgcagatc aaagagtggg
    14101 caccgttagt gatggaaggg cgcgatccgc aacagaaagt ggcagccacg cgtacggaaa
    14161 ttggtaccga tgtgaactac ggcgagatca cccgccagtt aattgcttcc ttgcagaaga
    14221 aatctaactt ctcgctgcaa ctcagcagcg aagtccgcgc cctaaagcgt aatgacgata
    14281 acacctggac cgttaccgtt gccgatctga aaaatggcac tgcacagaac attcgtgcca
    14341 aatttgtctt tatcggcgcg ggcggtgcgg cgctgaagct gttacaggaa tcggggattc
    14401 cggaagcgaa agactacgcc ggtttcccgg tgggcggaca gttccttgtt tcggaaaacc
    14461 cggacgtggt taatcaccat ctggcgaagg tttacggtaa agcatccgtt ggcgcaccac
    14521 cgatgtcggt tccgcatatc gatacccgcg ttctggacgg taaacgcgta gtgctgtttg
    14581 ggccatttgc caccttctca accaaattcc tcaaaaacgg ttcattgtgg gatctgatga
    14641 gttccaccac cacctctaac gtgatgccga tgatgcacgt cgggctggat aatttcgatc
    14701 tggtgaaata tctggtgagt caggtgatgt tgagtgaaga ggatcgtttt gaagcgttga
    14761 aagagtacta tccgcaggcg aaaaaagagg actggcgttt gtggcaagcg gggcagcgtg
    14821 tgcagattat caagcgtgat gccgataaag gtggtgtact gcgtctgggt actgaagtgg
    14881 tcagtgacca gcaagggacc attgccgcgc tcctgggagc atcgccaggg gcgtcaaccg
    14941 ccgcgccgat tatgttggat ctgctggaaa aagtatttgg cgatcgcgtt tccagcccgc
    15001 aatggcaggc tacgttgaaa gcgatcgttc cgtcttatgg acgcaagctg aacggtgatg
    15061 tagcggcaac agaacgcgag ttgcagtaca ccagcgaagt gctggggctg aaatacgaca
    15121 agccgcaggc agcagatagt acgccaaaac cgcagttgaa accgcaaccc gttcaaaaag
    15181 aagtggcgga tattgcgttg taatgatgtg ccacatccgg catggtatgc cggatgtggc
    15241 gtatactgat aagacgcgcc agcgtcgcat caggcaaccg gctcgggcgt tagatgttgg
    15301 atgcggcgta accgccttat ccaaccaacg gggtgccgta cttcacaaca gtttagcgaa
    15361 cttctgcgtt gtcaattttc tcttccgcct tccagacgcg gtatttcaca tctacattgt
    15421 ctggcgtata caccacgatc ggcagcttgc tgttgtaacg cagcattcca gcatcgccca
    15481 gatacgcggt gacaaatttc ttctctttct tgccatccgg gcaggccatc atcgtggaaa
    15541 ccggcgagct aactttatca aagacataat aatcatagcc ccagccttcc agcgttttgc
    15601 tttccagctt cccgccgaga cgatgcaaat tgcaatcgac ttccagcgtc tgaccgatta
    15661 acagttctac tttcagggta gattcatctt cttgcggggt taactgaatc acctgacgct
    15721 tcatcccttt ttcagcttgt ggataaggcg cgattttttc cagtggctgg acgctttctg
    15781 ccgcccaggc ggaagtggta gcgaaagcgg caaacaatac tgcaggtaga atggtcttca
    15841 tttttttgct ttccttcttc atttataggt tttcaccgat gatgtcgctg aaggttaaca
    15901 tattcataag atttattaat ttgctttatg aataacttaa agaatattca gacaatttta
    15961 cctgctcttt tagtgttaaa ttctgatgag agaacaaacc agcagaaata tctctggtga
    16021 attaatcgct atataaatat gtttataacc atttgaaatg tgagcaaaag cccgtttttg
    16081 ccacgcttcc cgccactctt ttgatcctgc tacaggtttt accccgatcg gggtatgcat
    16141 ctttgacaca tcctttaata tcttagcggt tataaaaatg gcttattaat tatgcggttt
    16201 atttggtcgc tctcaatttt cagagcgcgt taatgatgga aggtcaatgt gaagattgat
    16261 gcatcccgtc ggggcatact cactggtcgc tggcgcaaag ccagtaacgg tatccgtccg
    16321 ccctggtccg gtgatgaatc tcattttctg acccattgca cccgttgcga cgcctgtatt
    16381 aatgcttgtg aaaacaacat tctgcaacgc ggcgcgggcg gctatccgag cgttaatttc
    16441 aaaaataacg agtgcagctt ctgttacgct tgcgcgcagg cttgccccga atcgctattt
    16501 tctccgcgcc acaccagggc ctgggatttg cagttcacca ttggggacgc gtgcctggcg
    16561 tatcagtcag ttgaatgccg ccgctgtcag gatagctgtg agccaatggc aattatcttt
    16621 cgcccgacgc tctccgggat ctaccagccg caacttaata gccaactttg caacggatgt
    16681 ggagcatgtg cagccagctg tccggtatca gccataactg cggagtatct ccatgcacac
    16741 taactggcaa gtttgcagcc tggtcgtgca ggccaaaagc gaacgaattt cagacatcag
    16801 cacccaactg aacgcctttc ccggctgtga agttgctgtc agcgacgcgc cgagcggtca
    16861 gttgattgtg gtggtggaag cagaagacag cgaaacgctg atccaaacca ttgagtcagt
    16921 acgcaacgta gagggcgtgc tggcggtgtc gctggtttat caccagcagg aagagcaagg
    16981 tgaggaaaca ccatgaaact cagtcgtcgt agctttatga aagctaacgc cgttgcggcc
    17041 gctgcggcgg ctgccggtct cagcgtgccg ggcgttgccc gcgccgttgt tggtcagcag
    17101 gaagccatca aatgggataa agcgccgtgc cgtttctgcg gtactggttg cggcgttctg
    17161 gtcggaacgc agcagggacg tgtggtggcc tgtcagggcg acccagacgc accggttaac
    17221 cgtggcctga actgcattaa gggctatttc ctgcccaaga tcatgtacgg taaagaccgt
    17281 ttgacgcagc cgctgctgcg tatgaaaaac ggtaaatatg acaaagaagg cgaatttacc
    17341 ctaatcacct gggatcaggc cttcgatgtg atggaagaga aattcaaaac cgccctgaaa
    17401 gaaaaagggc cggaatcgat cggtatgttc ggttctggtc agtggactat ctgggaaggt
    17461 tatgccgcgt ccaagctgtt taaagcgggc ttccgttcga acaacatcga cccgaacgcg
    17521 cgtcactgta tggcgtcggc agtagttggc tttatgcgta cctttggtat ggatgagccg
    17581 atgggctgct atgacgacat cgagcaggct gacgcgtttg tgctgtgggg cgcaaacatg
    17641 gcggagatgc acccgatcct ctggtcgcgc atcactaacc gtcgtctctc taatcagaac
    17701 gtcaccgtgg cggtgctttc tacctaccag catcgtagct tcgagctggc ggataacggc
    17761 atcatcttta cgccacagtc tgacctggtg atcctgaact acatcgccaa ctatatcatt
    17821 caaaacaatg cgataaatca ggacttcttc agcaaacacg ttaacctgcg caaaggggcg
    17881 acggacattg gctacggttt acgtccgacc catccgctgg aaaaagcagc gaagaatcca
    17941 ggttctgacg cctccgaacc gatgagcttt gaagattaca aagccttcgt tgccgagtat
    18001 acgctggaaa aaactgccga aatgaccggc gtgccgaaag accagttaga acaactggcg
    18061 cagctgtatg ccgatccgaa caagaaagtc atctcctact ggacgatggg cttcaaccag
    18121 catactcgtg gcgtgtgggc taacaacctg gtctacaacc tgcacctgct gaccggcaaa
    18181 atttcccagc cgggttgcgg tccgttctcc ctgaccgggc aaccttctgc gtgtggtact
    18241 gcgcgtgaag tgggcacctt tgctcaccgt ctgcctgcgg acatggtggt gaccaacgag
    18301 aaacaccgtg atatctgcga gaagaagtgg aatatcccga gcggcaccat tccggcgaaa
    18361 atcggtctgc acgcggtggc acaagaccgt gcgctgaaag acggcaaact gaatgtttac
    18421 tggaccatgt gtaccaacaa catgcaggcc gggccgaaca tcaatgaaga gcgtatgccg
    18481 ggctggcgcg atccgcgcaa cttcatcatc gtctccgatc cgtatccgac agtcagtgcg
    18541 ctggccgccg acttgatcct gccgaccgca atgtgggtag agaaagaggg cgcttacggt
    18601 aacgccgaac gccgtactca gttctggcgt caacaggtac aggcaccggg cgaagcgaaa
    18661 tcggatctct ggcagttagt ccagttctcc cgccgcttca aaactgaaga agtatggccg
    18721 gaagatctgc tggcgaagaa accggaactg cgtggcaaaa cgctgtacga agtgctgtat
    18781 gccacaccgg aagtgagcaa attcccggta tccgaactgg cggaagatca gctgaacgat
    18841 gaatcccgcg agctgggctt ctatctgcaa aaagggctgt tcgaagagta cgcatggttt
    18901 ggtcgcggtc acggtcacga tctggcaccg ttcgatgact accacaaagc gcgcggtctg
    18961 cgctggccgg tggtgaacgg taaagaaacg cagtggcgtt acagcgaagg taacgacccg
    19021 tacgtgaaag cgggcgaagg ctataagttc tacggcaaac cggatggcaa agcggtgatc
    19081 ttcgcactgc cattcgaacc ggcggcggaa gcaccggatg aagagtacga cctgtggctc
    19141 tctaccggac gcgttctgga gcactggcac accggcagta tgactcgccg tgtaccagaa
    19201 ctgcaccgcg ccttcccgga agcggtcctg tttattcacc cgctggatgc gaaagcgcgc
    19261 gatctgcgtc gtggcgacaa agtgaaagtg gtttctcgcc gtggcgaagt gatctcgatt
    19321 gtcgaaacgc gcggtcgtaa ccgtccgccg cagggcctgg tgtacatgcc gttcttcgac
    19381 gccgcacagc tggttaacaa actaacgcag gacgcgaccg atccgctctc gaaagagacg
    19441 gacttcaaga agtgcgcagt caaactggag aaggtgtaag ccattatgtc ccggtcagcg
    19501 aaacctcaaa atggtcgccg ccgctttctg cgcgatgttg ttcgcacagc aggcgggctg
    19561 gctgccgtgg gtgtggcgct ggggttacaa cagcaaaccg cacgcgcatc tggcgtgcgg
    19621 ttgcgcccgc ccggagccat aaacgagaac gcctttgcca gtgcctgtgt gcgttgcggt
    19681 cagtgtgttc aggcttgccc ttacgacacc ttaaaactgg cgacgctggc ctctggtctg
    19741 tcggcgggca cgccatattt tgtcgcacgg gatattcctt gcgaaatgtg tgaggacatt
    19801 ccgtgcgcca aagtgtgccc gagcggtgcg ctggatcgtg agatcgaatc gatcgacgac
    19861 gcgcggatgg ggctggcggt actggtggac caggaaaact gtctcaactt tcaggggctg
    19921 cgctgcgatg tttgttatcg cgaatgcccg aaaattgatg aggccatcac cctggagctg
    19981 gagcgcaaca cgcgtaccgg taagcacgcc cgctttctgc cgacggttca cagagacgcc
    20041 tgtaccggtt gcggtaagtg cgaaaaagtg tgcgtgctgg aacaaccggc aatcaaggtg
    20101 ttaccgctgt cactggctaa aggggagtta ggtcaccatt accgcttcgg ctggctggag
    20161 gggaacaatg gcaaatcgta aacgtgacgc cgggcgcgag gcgctggaga aaaaaggctg
    20221 gtggcgcagt caccgttggc tggtgttacg tcgcctttgt cagttcttcg tgctggggat
    20281 gtttttgagc ggtccgtggt ttggtgtgtg gatcttacac ggtaactaca gcagtagcct
    20341 gttattcgac accgttccgc tgaccgatcc gctgatgacg ctgcaaagtc ttgccagcgg
    20401 tcatctgcct gccacggtgg cgttgaccgg tgcggtgatt atcaccgtgc tctacgccct
    20461 ggcaggaaag cgattatttt gcagctgggt ctgcccgctg aatccgatta ccgacttagc
    20521 gaactggtta cgcaggcgct ttgacctcaa tcagtctgcg acgatccctc gccacattcg
    20581 ctacgtgctg ttggtggtca ttctgctggg atcggcctta actggcacgc tcatctggga
    20641 atggatcaac cccgtatctc tgatggggcg cagcctggta atgggcttcg gcagcggcgc
    20701 gctgcttatt ctcgcgctgt ttttatttga tctactggtc gttgaacacg gctggtgcgg
    20761 gcacatttgt ccggttggcg cgctgtatgg cgtgctgggt agcaaaggtg tgattaccgt
    20821 tgcggccacc gatcggcaga aatgtaaccg ctgtatggat tgttttcatg tttgcccgga
    20881 accgcgtgtg ctacgtgccc cggtgctgga tgaacaaagc ccggtgcagg tcaccagccg
    20941 cgattgcatg acgtgcggtc gctgcgtgga tgtctgttct gaggatgtat ttacaataac
    21001 tacacgatgg agttcgggag cgaaatcatg aaaagccatg acctgaagaa agcgctgtgt
    21061 caatggacgg cgatgctggc cctggtggta agcggcgcgg tttgggcggc taatggcgtc
    21121 gattttagcc agtcgccgga agtatccggg acacaggaag gggccattcg gatgccaaaa
    21181 gagcaggaac ggatgccgct gaactatgtg aatcagccgc caatgatccc gcatagcgtt
    21241 gaaggttatc aggtaacgac caataccaac cgctgcttgc agtgccacgg tgtcgaaagt
    21301 tatcgcacca ctggcgcgcc gcgcattagt cctactcact ttatggacag cgacggcaaa
    21361 gtgggcgcgg aagtggctcc gcgtcgttat ttctgtctgc aatgtcacgt accgcaggcc
    21421 gataccgcgc caatcgtggg gaataccttt accccatcaa aaggttacgg gaaataagag
    21481 gttattatgg gaaattctga ccgtaagcct ggtctgatta agcgcctgtg gaaatggtgg
    21541 cgtaccccca gccgtctggc gctggggacg ctgctgttga tcggttttgt tggcggcatc
    21601 gtcttctggg gtggctttaa caccgggatg gaaaaagcca ataccgaaga gttctgcatt
    21661 agctgccacg aaatgcgcaa cacggtgtat caggaataca tggattccgt gcactacaac
    21721 aaccgtagcg gcgtccgtgc gacctgtccg gattgtcacg ttccgcacga gtttgtgccg
    21781 aagatgatac gcaagctcaa agcaagtaaa gagctgtatg gtaaaatttt tggcgttatt
    21841 gacacgccgc agaaatttga agctcatcgt ctgacgatgg cacagaatga gtggcggcgc
    21901 atgaaggaca ataactcgca ggagtgccgt aactgtcaca acttcgagta tatggataca
    21961 accgcccaga aatcggttgc cgcgaagatg catgaccagg cggtgaaaga tgggcaaacc
    22021 tgtattgatt gccataaagg gatagcgcac aagctgcccg atatgcgtga agtcgagcca
    22081 ggtttttaac agggttattg cgtgggtatg cttgaagcca gagagttact ttgtgagcgg
    22141 gatgaacgaa ccttatttag tggcttgtca tttacgctga acgcaggaga gtgggtacaa
    22201 atcaccggta gcaacggcgc ggggaagaca acgcttctcc gtttgctgac ggggttgtct
    22261 cgccctgacg caggcgaggt tctctggcaa gggcagccct tgcatcaggt acgcgacagc
    22321 taccatcaaa acctgttatg gataggccat cagccgggga tcaaaacccg gctgacggcg
    22381 ttagaaaatc tgcactttta tcatcgcgat ggcgataccg cacaatgtct ggaagccctg
    22441 gcgcaggccg ggcttgccgg attcgaagat attcctgtaa atcagctctc ggccgggcaa
    22501 caacgccgcg tcgctttagc gcgtctgtgg ctgacccgtg ccacgttatg gatcctcgac
    22561 gagcctttta ccgcgattga cgttaacggc gtcgatcgtc tgacccagcg tatggcgcag
    22621 catacggagc agggggggat tgtgattctg actacccacc agccgctcaa cgttgctgaa
    22681 agtaaaattc gccgcatttc actgacgcaa acgagggccg catgatgttc tggcgcattt
    22741 tccgtcttga gctgcgtgta gcgtttcgcc atagcgccga aatcgccaac ccgctgtggt
    22801 tcttcctgat tgtaattacc ctttttccgc tcagtatcgg tccggagccg caactgctgg
    22861 cgcgtattgc accgggcatt atctgggttg ctgcgctgct ttcatccttg ctggcgctgg
    22921 aacgactgtt ccgtgacgat ttgcaggacg gcagtcttga acaattgatg ttgttgccgt
    22981 tacccttgcc cgccgttgtg ctggcgaagg tgatggcgca ctggatggta accggtctgc
    23041 cgttactcat cctttcgcca ctggtagcaa tgctactggg aatggatgtt tatggctggc
    23101 aagtgatggc gctgacgctg ctgctgggaa cgcctacgct tggctttctc ggtgcaccgg
    23161 gtgtggcgtt gacagtggga cttaagcgcg gtggtgtgct gctcagcata ctggtgttac
    23221 cgctgactat cccattactc atctttgcca ccgccgcgat ggacgcggct tccatgcatt
    23281 tgcccgttga cgggtatctg gcaattttag gcgcgttgct ggcaggcacc gcgacattaa
    23341 gtccttttgc gacggcggca gcgttacgaa tcagcattca ataacgctgg attactgatt
    23401 tttgttcgag tctggtatcg aaactatgtg gaaaacactg catcaactgg cgatcccacc
    23461 acggctgtat caaatctgtg gctggtttat accgtggctg gcaattgcca gtgtggtcgt
    23521 gcttaccgtc ggctggatct ggggattcgg ctttgctccg gctgattatc agcagggaaa
    23581 tagctaccgc attatctacc tgcatgtgcc tgcggcgatc tggtcgatgg gtatttatgc
    23641 atcaatggcg gtggcggcgt ttattggcct tgtctggcag atgaaaatgg ccaacctggc
    23701 ggtggcggcg atggccccca ttggtgccgt gtttaccttt attgccctgg ttaccggctc
    23761 tgcatgggga aaaccgatgt ggggcacctg gtgggtatgg gatgcacgtc tgacttctga
    23821 actggtgctg ctgtttttgt atgtgggtgt gattgccctg tggcacgcct tcgacgaccg
    23881 ccgtctggcg ggccgtgcgg caggtatcct ggtgctgatt ggcgtggtga atctgccgat
    23941 tattcattac tccgtggagt ggtggaacac cctgcatcag ggatcaacgc ggatgcagca
    24001 aagtatcgat ccggcgatgc gttcgccgct gcgctggtcg atttttggct tcctgctcct
    24061 gtctgccacg ctgacgctga tgcggatgcg taatttgatt ttgctgatgg aaaaacgccg
    24121 tccgtgggtg agtgaactga tactgaaaag aggccgtaaa tgacccttgc atttgcttcc
    24181 tggaatgaat ttttcgcaat gggcggttac gccttttttg tctggctggc ggtggtgatg
    24241 accgttattc cgctggtggt tttggtcgtg cactcggtga tgcagcatcg cgcaattctg
    24301 tgtggtgtgg agcaacagcg ggcgcgtgag gcgcgtttac gtgctgcgca acagcaggag
    24361 gctgcatgaa tattcgccgt aaaaaccgct tgtggattgc ctgtgctgtg ttggcaggtt
    24421 tggcgctgac catcggtctg gtgctgtatg cgctgcgctc gaatatcgat ctcttttata
    24481 cgccggggga aattctctac ggcaagcgtg aaactcagca aatgccggaa gtcggtcagc
    24541 gtctgcgcgt tggcgggatg gtgatgccgg gtagtgtgca gcgcgatccc aattcgctga
    24601 aagtgacctt caccatttac gacgctgaag gctcagtgga tgtctcttac gaaggcattt
    24661 tgccggatct gttccgtgaa gggcagggcg ttgtggtgca gggcgagctg gaaaaaggca
    24721 atcatatcct cgcgaaagaa gtgctggcga aacacgacga aaactacacg ccgccagaag
    24781 ttgagaaagc gatggaagct aaccaccgtc gcccggcgag tgtttataag gacccagcat
    24841 catgatgccg gaaattggaa acgggctgct gtgcctggcg ctgggaattg cgctgctgct
    24901 gtccgtgtat ccgctatggg gcgtggcgcg cggagatgcg cgcatgatgg cgtcttcccg
    24961 cttgtttgcc tggatgctgt ttatgtctgt ggctggcgca tttctggtgc tggtcaatgc
    25021 tttcgtggtc aacgacttta ccgtcaccta tgttgccagc aactccaata cccagcttcc
    25081 ggtgtggtat cgcgtggcgg ctacctgggg cgcgcatgaa ggttcgcttc tgctgtgggt
    25141 gctgctgatg agcggctgga cctttgcggt ggcgattttt agtcagcgta ttccgctgga
    25201 tattgtggcc cgtgtactgg cgataatggg gatggtcagc gtcggctttt tgctgttcat
    25261 tctctttacc tctaacccgt tctcacgcac gctgccgaac ttcccaattg aagggcgcga
    25321 tcttaacccg ctgttacagg atccggggct gatcttccat ccgcctctgc tttatatggg
    25381 gtacgtgggt ttctcggtgg cgtttgcttt tgccattgcc attgcttctt tgctgagcgg
    25441 gcgtctggac agcacttatg cgcgttttac tcgcccgtgg acgctggcgg cgtggatttt
    25501 cctgacgctc ggcattgtgc tcggttccgc atgggcctat tacgaactcg gctggggggg
    25561 ctggtggttc tgggatccgg tagaaaacgc ctcgtttatg ccgtggctgg tggggactgc
    25621 gctgatgcac tcactggcgg tcactgaaca acgcgccagc ttcaaagcgt ggacattact
    25681 gctggcaatc agtgccttct cgttgtgtct gctggggact ttcctggtgc gttccggcgt
    25741 gctggtatcg gtacacgcgt ttgcctctga tccggcacgc ggtatgttta tcctcgcctt
    25801 tatggtactg gtgattggtg gttcgctgct gctgtttgcc gcgcgtggac acaaagttcg
    25861 ctcacgcgta aacaatgcgc tgtggtcgcg ggaatctctg ctgttagcga acaacgtttt
    25921 gttggtcgcc gcgatgctgg tggtattact ggggacgctg ctgccgctgg tgcacaagca
    25981 actgggactg ggcagtattt cgattggcga accgttcttc aacaccatgt ttacctggct
    26041 gatggtgccg tttgcgctgc tgcttggtgt cggtcctctg gtgcgctggg ggcgcgatcg
    26101 cccacgtaaa atccgcaatt tattgattat cgccttcatc tcaacgctgg tgctgtcgct
    26161 gctgctgccg tggctgttcg aaagcaaagt tgtggcgatg acggtgctcg gcctggcaat
    26221 ggcctgctgg attgctgtgc tggcaattgc ggaagctgcg ctgcgtattt cacgtggcac
    26281 gaaaaccacc ttcagttatt ggggaatggt ggcggctcac ctggggctgg cagtgacaat
    26341 tgttggtatt gcctttagcc agaactatag cgttgagcgt gatgtgcgca tgaagtccgg
    26401 cgatagcgtc gatattcatg aatatcgctt caccttccgt gatgtcaaag aggtgactgg
    26461 cccgaactgg cgcggcggtg tggcgactat tggcgtaacg cgagagggta aaccggaaac
    26521 ggtgctgtat gcggaaaaac gttattacaa cactgccggg tcgatgatga ccgaagcggc
    26581 gattgacggc ggcatcacgc gtgacctgta cgccgcgctc ggtgaagagc tggaaaacgg
    26641 cgcgtgggcc gtgcgtcttt actacaaacc gtttgttcgc tggatttggg cgggcgggct
    26701 gatgatggcg ttgggcggac tgctgtgtct gtttgatcct cgctatcgta agcgcgtgag
    26761 tccgcaaaaa actgcgccgg aggctgtatg aagcgcaaag tattgttaat tccgttgatt
    26821 atcttcctgg cgattgccgc ggcgctgctg tggcagctgg cgcgtaatgc cgaaggggat
    26881 gatccgacca atctggaatc ggcgctcatt ggcaagccag tgccgaagtt tcgcctcgaa
    26941 tcactggaca atccggggca gttttatcag gcggatgtgc tgactcaggg caaacctgtg
    27001 ctgcttaacg tttgggctac ctggtgtccg acctgccgtg cggaacatca atatctgaat
    27061 cagctttctg cgcagggcat tcgcgtggtc ggcatgaact ataaagacga tcgccagaag
    27121 gcaataagct ggctgaaaga gctgggcaat ccttacgcgc taagcctgtt tgatggcgat
    27181 ggcatgttag ggctggatct cggtgtctat ggcgcgccag aaacgttcct catcgacggc
    27241 aacggcatca ttcgctatcg ccatgcgggc gatctcaatc ctcgcgtctg ggaagaagag
    27301 atcaagccgc tgtgggagaa atacagtaag gaggccgcac aatgaggttt ttattgggcg
    27361 tgctgatgct gatgatctcc ggctcagcgc tggcgaccat cgacgtgttg cagtttaagg
    27421 atgaagcgca ggagcagcag ttccgccaac tcactgaaga actacgctgc ccgaaatgcc
    27481 agaacaacag cattgccgat tccaactcga tgattgccac cgacctgcgc cagaaagtgt
    27541 atgaactgat gcaggaaggt aaaagtaaaa aagagattgt cgattatatg gtggcgcgtt
    27601 acggcaactt cgtcacttac gatccgccgt taacgccgct gaccgtgctg ctgtgggtgc
    27661 ttccggtagt ggctattggc attggcggtt gggtcattta cgcccgttcg cggcgtcggg
    27721 tacgcgtggt gccggacgcg tttcctgaac aaagcgtgcc ggaaggtaag cgtgccggat
    27781 atattgttta tctgccgggt attgtggtgg cgttaattgt ggctggcgtc agctactacc
    27841 agactggcaa ttatcagcag gtgaaaatct ggcagcaggc cacggcacag gctccggcgt
    27901 tgctggacag ggcgctggat ccgaaagccg atccgctcaa cgaagaagag atgtcgcgtc
    27961 ttgcgctggg gatgcgtact caactgcaaa aaaatccggg agatatagaa ggctggatta
    28021 tgttgggccg cgttggcatg gcgctgggta acgccagtat cgccaccgat gcatacgcta
    28081 ctgcgtatcg cctcgatccg aaaaacagtg atgccgcact gggttatgct gaagcgttga
    28141 cacgttcatc tgatcccaac gacaaccgcc tcggtggtga actgctgcgc cagttggtga
    28201 gaacggacca tagcaatatc cgtgtgttaa gcatgtatgc gtttaatgcc tttgagcagc
    28261 agcgatttgg cgaagccgtt gccgcgtggg agatgatgtt gaaactctta tctgccaacg
    28321 atactcgccg tgcggtgatt gaacgtagta tcgcgcaggc gatgcaacat ttgtcgccgc
    28381 aggagagtaa ataaaaagag gcccgatggt gatgccatcg ggctattttt attgtgcccc
    28441 gcgttgttgc aggaacagaa tggtggctgc cacgcgtgag cggacattga gtttgcgcag
    28501 cagattgcga atatgtactt ttactgtctg ctctgaaata ttcaacaccg aggcaatctg
    28561 tttatttgac agcccctgtg ccagctcgtg cagaacatcc agctcgcgct ccgtcagcac
    28621 gctgaaggga tcttcttccg cgccaaacat ttcacgttca cgtaagtact gattgacgcg
    28681 ttcgctaaag actttgctgc ctttcgctcc agcacgaatt gcttccagca atacttccgg
    28741 gtcgctgtct ttcaacagat aaccgtctgc gcctgcgtct atcagcgcaa agacatcgct
    28801 ggaggcatct gatacggtca ggataataat ttgcgcggta acgccatccc tgcgcaaggc
    28861 attgagggta tccaggccac tcataccttt catattgaga tccagcaaga tcacgtcgat
    28921 atccagtcta tttgccagat cgatcgcgct cgcgccgtcg cccgcttcgg cgaccacttc
    28981 aaagctagga tcaagctcca gtaactgacg aacaccgcgt cgcataagtg gatgatcatc
    29041 cacaatcatc acctgaaaag gcgttgcttc aggcatagta gtctcctggg gttttattag
    29101 aatcattatg tttgagcgta gcgttggtaa cgctaccagg cgcgtgaatg ttaccccatt
    29161 ttatgtatgg acatgtagtg aaaactacaa ccttgtcgtt cgctttggca tcattttcat
    29221 tataaagacg gaattttgca tcagcttctc
//
LOCUS       DAJTOR010000033        29048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTOR010000033 DAJTOR010000000
VERSION     DAJTOR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.033"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            162..605
                     /locus_tag="OO249_001310"
     CDS             162..605
                     /locus_tag="OO249_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5635671.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLTDVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            841..1659
                     /gene="ybhA"
                     /locus_tag="OO249_001311"
     CDS             841..1659
                     /gene="ybhA"
                     /locus_tag="OO249_001311"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5635672.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1660..2718)
                     /gene="modC"
                     /locus_tag="OO249_001312"
     CDS             complement(1660..2718)
                     /gene="modC"
                     /locus_tag="OO249_001312"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5635673.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2721..3410)
                     /gene="modB"
                     /locus_tag="OO249_001313"
     CDS             complement(2721..3410)
                     /gene="modB"
                     /locus_tag="OO249_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5635674.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3410..4183)
                     /gene="modA"
                     /locus_tag="OO249_001314"
     CDS             complement(3410..4183)
                     /gene="modA"
                     /locus_tag="OO249_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5635675.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4349..4498)
                     /gene="acrZ"
                     /locus_tag="OO249_001315"
     CDS             complement(4349..4498)
                     /gene="acrZ"
                     /locus_tag="OO249_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5635676.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4627..5415
                     /gene="modE"
                     /locus_tag="OO249_001316"
     CDS             4627..5415
                     /gene="modE"
                     /locus_tag="OO249_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5635677.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5483..6955
                     /gene="modF"
                     /locus_tag="OO249_001317"
     CDS             5483..6955
                     /gene="modF"
                     /locus_tag="OO249_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5635678.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKILLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7216..8232
                     /gene="galE"
                     /locus_tag="OO249_001318"
     CDS             7216..8232
                     /gene="galE"
                     /locus_tag="OO249_001318"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5635679.1"
                     /translation="MRVLLTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8242..9288
                     /gene="galT"
                     /locus_tag="OO249_001319"
     CDS             8242..9288
                     /gene="galT"
                     /locus_tag="OO249_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5635680.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9292..10440
                     /gene="galK"
                     /locus_tag="OO249_001320"
     CDS             9292..10440
                     /gene="galK"
                     /locus_tag="OO249_001320"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5635681.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10434..11474
                     /gene="galM"
                     /locus_tag="OO249_001321"
     CDS             10434..11474
                     /gene="galM"
                     /locus_tag="OO249_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5635682.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11676..12428
                     /gene="gpmA"
                     /locus_tag="OO249_001322"
     CDS             11676..12428
                     /gene="gpmA"
                     /locus_tag="OO249_001322"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5635683.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12586..13638)
                     /gene="aroG"
                     /locus_tag="OO249_001323"
     CDS             complement(12586..13638)
                     /gene="aroG"
                     /locus_tag="OO249_001323"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5635684.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13954..14334
                     /locus_tag="OO249_001324"
     CDS             13954..14334
                     /locus_tag="OO249_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5635685.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14448..15389
                     /gene="zitB"
                     /locus_tag="OO249_001325"
     CDS             14448..15389
                     /gene="zitB"
                     /locus_tag="OO249_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5635686.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHAHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15386..16105)
                     /gene="pnuC"
                     /locus_tag="OO249_001326"
     CDS             complement(15386..16105)
                     /gene="pnuC"
                     /locus_tag="OO249_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5635687.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(16143..17186)
                     /gene="nadA"
                     /locus_tag="OO249_001327"
     CDS             complement(16143..17186)
                     /gene="nadA"
                     /locus_tag="OO249_001327"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5635688.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17435..17510)
                     /locus_tag="OO249_001328"
     tRNA            complement(17435..17510)
                     /locus_tag="OO249_001328"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17475..17477),aa:Lys,seq:ttt)
     gene            complement(17657..17732)
                     /locus_tag="OO249_001329"
     tRNA            complement(17657..17732)
                     /locus_tag="OO249_001329"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17697..17699),aa:Lys,seq:ttt)
     gene            complement(17736..17811)
                     /locus_tag="OO249_001330"
     tRNA            complement(17736..17811)
                     /locus_tag="OO249_001330"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17776..17778),aa:Val,seq:tac)
     gene            complement(17863..17938)
                     /locus_tag="OO249_001331"
     tRNA            complement(17863..17938)
                     /locus_tag="OO249_001331"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17903..17905),aa:Lys,seq:ttt)
     gene            complement(17941..18016)
                     /locus_tag="OO249_001332"
     tRNA            complement(17941..18016)
                     /locus_tag="OO249_001332"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17981..17983),aa:Val,seq:tac)
     gene            complement(18052..18127)
                     /locus_tag="OO249_001333"
     tRNA            complement(18052..18127)
                     /locus_tag="OO249_001333"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18092..18094),aa:Lys,seq:ttt)
     gene            complement(18292..19083)
                     /gene="cpoB"
                     /locus_tag="OO249_001334"
     CDS             complement(18292..19083)
                     /gene="cpoB"
                     /locus_tag="OO249_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5635689.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(19093..19614)
                     /gene="pal"
                     /locus_tag="OO249_001335"
     CDS             complement(19093..19614)
                     /gene="pal"
                     /locus_tag="OO249_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5635690.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19649..20941)
                     /gene="tolB"
                     /locus_tag="OO249_001336"
     CDS             complement(19649..20941)
                     /gene="tolB"
                     /locus_tag="OO249_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5635691.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLVFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(21074..22255)
                     /gene="tolA"
                     /locus_tag="OO249_001337"
     CDS             complement(21074..22255)
                     /gene="tolA"
                     /locus_tag="OO249_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5635692.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAK
                     AEAEKKAAAEKAAADKKAAADKKAAEKAAAEKAAADKKAAAEKAAAEADDIFGELSSG
                     KNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKT
                     CTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFK
                     P"
     gene            complement(22320..22748)
                     /gene="tolR"
                     /locus_tag="OO249_001338"
     CDS             complement(22320..22748)
                     /gene="tolR"
                     /locus_tag="OO249_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5635693.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22752..23444)
                     /gene="tolQ"
                     /locus_tag="OO249_001339"
     CDS             complement(22752..23444)
                     /gene="tolQ"
                     /locus_tag="OO249_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5635694.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23441..23845)
                     /gene="ybgC"
                     /locus_tag="OO249_001340"
     CDS             complement(23441..23845)
                     /gene="ybgC"
                     /locus_tag="OO249_001340"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5635695.1"
                     /translation="MNTTLFRWPARVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23995..24288)
                     /gene="ybgE"
                     /locus_tag="OO249_001341"
     CDS             complement(23995..24288)
                     /gene="ybgE"
                     /locus_tag="OO249_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5635696.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            complement(24288..24401)
                     /gene="cydX"
                     /locus_tag="OO249_001342"
     CDS             complement(24288..24401)
                     /gene="cydX"
                     /locus_tag="OO249_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5635697.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24416..25555)
                     /gene="cydB"
                     /locus_tag="OO249_001343"
     CDS             complement(24416..25555)
                     /gene="cydB"
                     /locus_tag="OO249_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5635698.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25571..27139)
                     /gene="cydA"
                     /locus_tag="OO249_001344"
     CDS             complement(25571..27139)
                     /gene="cydA"
                     /locus_tag="OO249_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5635699.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27987..>29048)
                     /locus_tag="OO249_001345"
     CDS             complement(27987..>29048)
                     /locus_tag="OO249_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5635700.1"
                     /translation="SSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIRYD
                     MAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTPFN
                     TDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVFSE
                     GLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSCRL
                     SLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPPVG
                     CLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQGS
                     NLSGPFLPGQSRTFSYRLA"
BASE COUNT         7339 a         7635 c         7085 g         6989 t
ORIGIN      
        1 acatttttaa aacagctata gcttctggtc ttgccgaaga agatccagct tctgcaacca
       61 ggccattcag aactgaagtg aagcgcagtc gactgttaat tgacgagtat ctgttaatca
      121 ggaaaatagc agacgtccag aacgaatggt tcggcctttg catggatctt gcactggtaa
      181 caggacagcg cgaaggagat ctggcggcta tgagatggga ggatataaga gatggcagac
      241 tatacgtgga acaacaaaaa acaggggcaa aaattcgcat ttctctcccc accacgattt
      301 ccagattgaa tttaacactt accgatgtac tggataactt gaaaaaaata aacggtaaaa
      361 acgaaaagtt actcggaggg aagacagcca ggacaatcgc agcacagttc aggattgccc
      421 gagacacatc cggattgaaa tgggaaggtg acccaccgcc atttcatgaa atccgaagtt
      481 tatccggtcg cctgcactca gcagaaaaag gcagtgattt cactcaggcg ctccttggac
      541 atcgttcatc cagcatgacg gataaatacc gtgacggacg agggagggaa tggaaagaca
      601 tttagaatta atttcgaatg atttcgaaac accacttgta attaattgat tataaaggat
      661 taattcctaa gaggcatttg cttcatgaat gctcctttgc atttagcaac tgactgtaac
      721 agctaaaatt agtcgctttt ggcggtgaat gctgaattaa aacgcagaat tttgtccgat
      781 agtggtatcg gtgtaccatt cgcgcagacg aaaattctta accttaatct ggaatacgcc
      841 atgaccacac gcgtgattgc tctcgactta gacggcacct tattgacccc gaaaaagacc
      901 ctgcttcctt catcgataga agccctggcc cgcgctcgcg aagcaggcta tcaattaatc
      961 atcgtcacag gtcgccatca cgtcgctatt catccttttt atcaggcgct ggcgctggat
     1021 acacctgcta tttgctgtaa tggcacctat ttgtatgatt atcatgcaaa aaccgtgctg
     1081 gaagcggacc caatgcccgt taataaagcc ctgcaactca ttgagatgct gaatgaacac
     1141 cacattcacg gtctgatgta tgtggatgat gcaatggtct atgagcaccc gaccgggcat
     1201 gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg aacagcgtcc gactttcaca
     1261 caagtcgctt ctctggctga aacggcgcaa caagttaacg ccgtatggaa gttcgccctg
     1321 acgcacgatg acatgccgca attgcagcat tttggtaagc atgtcgaaca tgaactggga
     1381 ctggagtgtg aatggtcctg gcacgatcag gttgatattg cacgcggcgg caacagcaaa
     1441 ggtaaacgtt tgacgaaatg ggttgaggcg caaggctggt cgatggaaaa cgtcgtggcg
     1501 ttcggcgata actttaatga tatcagtatg ctggaggccg ctggtacagg cgtggcgatg
     1561 ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
     1621 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
     1681 tttaatttgc gcgtacagcc acaggcctgg tttgatcgcc agttcatccc tggcccacgg
     1741 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctgaccgtt
     1801 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
     1861 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1921 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1981 gtaatgcgga tgatgctcaa gcaccgtcac tttcagaatg ctactttgtt gctctttcgg
     2041 cagccacgga ttcatcacgc tactacccca gacttcttcc agtgcgccaa aggctttcac
     2101 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     2161 gctgacatac aacatcggga tgttgatttc ccgcgtcaga cgttgcagat aaggcaacag
     2221 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattctgg
     2281 tgccgtcagc aaagcccgcc caatcgctac gcgctgtttt tcgcctccgg acaggctgcc
     2341 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     2401 gaccatgctt ttcgccatgc cgtagcgcag attgccacgc actttgtaat gcggaaacag
     2461 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttct ggcgtcaggc agataccttt
     2521 ttcggcatca tttagtaccc gaccattgag gacaatcctc cctttttgcg ggcgcgtcag
     2581 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     2641 gatgccattg gcgggcagcg tttcattaat agtcaggcaa tggttgccca acgtctggga
     2701 aaaattcagt tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     2761 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     2821 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     2881 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2941 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     3001 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     3061 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     3121 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     3181 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     3241 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     3301 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     3361 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     3421 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     3481 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     3541 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     3601 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     3661 cttccgccgg agccaatttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     3721 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     3781 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     3841 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3901 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3961 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     4021 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     4081 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     4141 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     4201 aacgttatat aggtgacaat ataacggcta actcagaatt ttcccagcag ttattgctaa
     4261 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     4321 atgcccgact aagtgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     4381 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     4441 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     4501 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     4561 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     4621 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     4681 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     4741 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     4801 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     4861 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4921 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4981 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     5041 gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     5101 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     5161 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     5221 gccaacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     5281 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     5341 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     5401 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     5461 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     5521 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     5581 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     5641 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     5701 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     5761 tggcgaagat gacaccggac gcactacggc tgagatcatt caggatgaag taaaggatgc
     5821 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     5881 taaatacctt tccactggcg agacgcgaaa aatcctgctg tgtcaggcgc tgatgtcgga
     5941 gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct cacgtcagca
     6001 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     6061 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     6121 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcaca
     6181 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     6241 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     6301 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     6361 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     6421 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     6481 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     6541 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     6601 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     6661 tacggctgac gctccgttcc atagtctttc ctggggacag cagcatctgg cgctgatcgt
     6721 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     6781 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     6841 gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6901 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6961 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     7021 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     7081 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     7141 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     7201 ctaatggagc gaattatgag agttctgctt accggtggta gcggttacat tggaagtcat
     7261 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     7321 agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc aacgtttgtt
     7381 gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca cgctatcgac
     7441 accgtgatcc acttcgccgg gctgaaagcc gttggcgaat cggtacaaaa accgctggaa
     7501 tattacgaca acaatgtcaa cggtactctg cgcctgatta gcgccatgcg cgccgctaac
     7561 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     7621 tacgttgaaa gcttcccgac cggcacaccg caaagccctt acggcaaaag caagctgatg
     7681 gtggaacaga tcctcaccga tctgcaaaaa gcccagccgg actggagcat tgccctgctg
     7741 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     7801 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     7861 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7921 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7981 gtacacatct acaacctcgg cgctggcata ggcagcagcg tgctggacgt ggttaatgcc
     8041 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgagggcgac
     8101 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     8161 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     8221 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     8281 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     8341 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     8401 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc acgattacac cgggacttac
     8461 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     8521 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     8581 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     8641 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     8701 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     8761 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     8821 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     8881 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8941 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     9001 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     9061 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     9121 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     9181 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     9241 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     9301 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     9361 caggcgcctg gccgcgtgaa tttgattggt gaacacacgg actacaacga cggtttcgtt
     9421 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     9481 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     9541 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     9601 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     9661 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     9721 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     9781 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     9841 gatcatgcct tgctgattga ctgtcgttca ctggggacca aagcagtttc catgccgaaa
     9901 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9961 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agccctgcgc
    10021 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
    10081 cgcgtgcggc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
    10141 cagggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
    10201 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agctgtgatt
    10261 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgttg
    10321 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
    10381 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
    10441 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
    10501 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gcccgtattc
    10561 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
    10621 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
    10681 gccgttatac ctttgacggt gaaaccgtga ccctttcgcc aagtcagggc gttaaccagc
    10741 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
    10801 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
    10861 gcgcaacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10921 cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10981 agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat ctgccggttg
    11041 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacgtct tttgatttcc
    11101 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    11161 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    11221 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg gctctgcaat
    11281 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aacagaacct tacgccgact
    11341 ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    11401 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    11461 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atctcatatt gctcaaatca
    11521 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    11581 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cgcggcaata taatgagaat
    11641 tattatcatt aaaagatgat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    11701 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    11761 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    11821 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    11881 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11941 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aactgctgaa aagtatggcg
    12001 acgagcaggt gaaacagtgg cgtcgtggtt ttgcagtgac tccgccggaa ctgactaaag
    12061 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagaactgc
    12121 cgctgacgga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    12181 ttctaccgcg tatgaagagc ggtgagcgcg taatcatcgc cgcacacggt aactccctgc
    12241 gtgcgctggt gaaatatctt gataacatga gcgaagaaga gattcttgag ctgaatatcc
    12301 cgactggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    12361 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgcaaac cagggtaaag
    12421 cgaagtaaac gtcattcgtt taaaatgaga aagccgactg caagtgagtc ggcttttttg
    12481 ttgctaacaa tggagcacat tgcctgatgc gacgcttcgc gacttatcag gcctgtggtg
    12541 attcatcgga tacgccactc tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc
    12601 ttttactgca ttcgccagtt gacgtaacag agcatcggta tcttcccagc cgatgcaggc
    12661 atcggtgatg ctcttaccgt aggccagcgg ctccccgctc tcgaggctct gattgccttc
    12721 caccagatgg ctttccacca tcacgccaat aatggccttt tcgccaccgg caatctgctg
    12781 gcaaacgtca gcacaaacat ccatctgctt tttgaattgt ttggacgagt tagcatggct
    12841 gaaatcgatc atcacctgtg ctggcaggcc tgctttgttc agcccttctt tcacttcagc
    12901 aacgtgcttc gcgctgtagt taggctcttt accgccgcgc agaatgatat ggcaatcgcc
    12961 gttaccgctg gtattcacaa tcgccgaatg cccccatttc gttacggaca ggaagcagtg
    13021 cggcgcaccg gcggcattaa tggcatcgat agccacttta atcgtaccgt cggtgccatt
    13081 tttgaagccg actggacaag aaagccctga tgccagttcg cggtgcacct gcgattcggt
    13141 ggtacgtgcg ccaattgcgc cccagctcat caggtcagcg agatattgtg gggtgatcat
    13201 atcgagaaac tcacccgccg ctggcagacc gctgtcgtta atatcaagca gcaatttacg
    13261 ggctatacga agaccgtcgt tgatctggaa gctgttatcc atatgcggat cgttaatcag
    13321 ccctttccag cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta cgatttccag
    13381 ctcatctttc agctcttcac gcagcgccag caagcgagtg gcatactctt tagccgcgac
    13441 aggatcatga attgagcacg ggccaatcac caccaacagg cgatcatcat tacctttcag
    13501 gatcttatgg atcgcttttc gggcatgggc aaccgtattc gcggcatttt cagtagcggg
    13561 gaatttttcc agcaatgcga caggaggaag taactctttg atttctttga tgcgtaaatc
    13621 gtcgttctga taattcatgt ctgttccagt gttgccatac ttatcttagt gaatgcaata
    13681 cttccaatct atatcttccg tcagaatgtg taaacggggt tttacattat gaacggatta
    13741 atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct ctcctacaat
    13801 ttctaactgt aactcctttg tttgttaatt atttcaagat tctctgctgc gtttcataaa
    13861 ctggctgaaa tcttaaacca atgccttata ttcacctgca aatgcactgt tggaagaggt
    13921 tatccgacat aacgaccata acaggagcat cctatgaaaa tgacaaaact ggccacatta
    13981 tttctgactg ccactctaag ccttgccagc ggtgccgcac tggctgccga tagcggagcg
    14041 caaactaata acggccaggc aaacgccgca actgatgcgg gccaggtagc ccctgacgcc
    14101 cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg taaataccgg ttctggcggc
    14161 acaatgctgc attcggatgg ttcttcaatg aacaatgacg gaatgaccaa agatgaagag
    14221 cacaagaaca ccatgtgcaa agatggtcga tgtccggata tcaataaaaa ggtacaaacc
    14281 ggtgatggca tcaataatga tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc
    14341 aaaatagcca ataatgggag agcggagtct ctccctttta atttttcact tctaaaagca
    14401 gtatgatgtc ataaagttaa gtgagaataa ttaagggatg actcgtcatg gcgcactcac
    14461 actcacacac gtcctcacac ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt
    14521 tcggcgtgac tgccgggttt atgctggtag aagtcgttgg tggttttctt tctggttctc
    14581 tggcattgct ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg cttttcgccc
    14641 tactcgccgt gcaattttca cgtcgtcctc ccaccattcg ccacactttc ggttggctaa
    14701 gactgaccac gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt accattttga
    14761 ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc ggtcgagggc ggcatgatga
    14821 tggcaattgc cgtggctggg ttgctggcaa atatactttc tttctggtta cttcatcacg
    14881 gcagcgaaga gaaaaacctc aacgtacgag cagcggcatt gcatgtgctc ggggacctgt
    14941 tgggttcggt tggggctatt attgccgcgt tgattattat ctggaccggc tggacacctg
    15001 ctgaccccat tctctcgata gtggtgtcgc ttctggttct gcgtagcgcg tggcgactgt
    15061 tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat atcgctgaac
    15121 tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat
    15181 ggatggtagg tgagaagccg gtgatgacgc tgcatgtgca ggtgatcccg ccacacgctc
    15241 acgatgcctt gttggatcag atccaacact acctgatgga tcactatcag attgagcacg
    15301 ccaccattca gatggaatat caaccttgtc atgggccaga ctgccacctg aatgaagggg
    15361 tgtctggcca ttcacatcat caccattaat gggacagcgc gcatgagcct ctttcacgtg
    15421 cgctgttgat ccacatccga ctgccgttga gcgcaataaa ggtcaggatg atgtactcca
    15481 gagacatggc gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg ttaataatca
    15541 cccacaacag ccagttttcc acatacttac gcgtcatcag aagcattgcc acgatagata
    15601 acaccatcat gcatgaatcc cagaacggga aagcgtccgg ttgcagttca ggcatcacaa
    15661 cctgtaatcc taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg
    15721 gattgataaa taccgtcatc agaccaatcg aaacaacgca aaccgccaac cagctgagcg
    15781 ccttcggcaa tggtaaccag cgaattttca actccgcctc gttctgactg gtttgtcgcg
    15841 accacgcata ccaaccgtaa atattcgcgg caaagaaaaa cacctgtaat agcaggctgg
    15901 catacagctg aatctgaaag aaaataatgc caaacaaggt gacgttgatc aggccaaaga
    15961 agtagttgct gatcttctcc agactggcaa ggccaataca cagcaacccg gcgatcgtgc
    16021 ctaccgcttc gatccatgag agatcataac cgcctgctcc tattggtata tgtaccagga
    16081 tattctgcac actaaaaaaa tccatctttc ccccaaagcg taacgttacg ccttattatt
    16141 cgttatccac gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac cagcgccctc
    16201 tctcgcagcc tttcatcaac atgaacctcg tgattgcttc cttcctgttc taatgcctct
    16261 gcgatggcct gaaggccatt catggccatc cacggacaat gcgcgcagct gcggcaggtt
    16321 gcaccctcac ctgcggttgg tgcttccagt aactctttat ctggcaccgc ctgctgcatt
    16381 ttgtagaaaa taccccgatc ggttgccaca ataagcctct gatgtggcaa tgttttcgca
    16441 gcagcgatca gttgactggt ggaaccgacc gcatccgcca tatcgacaat agcttgtggt
    16501 gattctggat gcaccagtat ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc
    16561 tgagtcttaa attcatcatg cacaatacag gcaccctgcc agcatagaat gtctccaccc
    16621 gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg cccagatgat tttttcaccc
    16681 aaactatcaa gatgatcaat aagttcgacg gcaatgcttg aagttaccac ccaatctgcg
    16741 cgcgctttta ccgcagcaga agtgttggcg tagacgacga cagtacgatc gggatgggca
    16801 tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat ccagtgaaca ttcagcctga
    16861 agtgtcggca tcagaattgt tttttccgga ctgagaattt tggcggtttc tcccataaat
    16921 ctcaccccag cgactaacaa agtagaagcg ggatgctttg caccgaagcg cgccatttcc
    16981 agagaatcag aaatacagcc accggtttct tctgccagtt gttgaatttc gggatcggta
    17041 tagtagtggg caaccatcac cgcattacgt tcttttagca gacgttttat cttctcgcgg
    17101 taatacgctt tttcatcaat gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc
    17161 gtgtctggat caaacattac gctcatctta ccatctcgtt ttacaggctt aacgttaaaa
    17221 ccgacattag cgtaatattc gctgttatcg gtgtaatttg ttttatatgc taaacaagat
    17281 agtcgaatgg tggggaaaag tcactcgaat tacgcctaat tccaccgacg tagagttgct
    17341 tgcacagtaa gctctgacga tgtcatcagg tttacaaagt gaaattgagg atgcctgaat
    17401 agcaaaaaag cgcctttagg gcgctttttt acattggtgg gtcgtgcagg attcgaacct
    17461 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc atacgggtgt
    17521 tacctgaaag atttctactc ggggccaatc tttaagattg gtgtgtcgtg caggatgact
    17581 cgcttcgctt gccctacggg ccgttgctgg cgcaacgtta tccttcacgc ttaacatctg
    17641 agtttgatgt taaaattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17701 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17761 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17821 ctggatacta caaactactt ccaccgaacc catcgtaatt agtggtgggt cgtgcaggat
    17881 tcgaacctgc gaccaattga ttaaaagtca actgctctac cgactgagct aacgacccgg
    17941 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    18001 gctgagccaa tcacccaata ctacgctgga tactacaact aactatgtta gtggtgggtc
    18061 gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta
    18121 acgacccact ttttcgttgc tttcggtttg tttgatatcc cgtggcaacg gcggcatata
    18181 ttactgattt cagacttgag cgcaacaaaa atttcgatgc agatcactca actgcttatg
    18241 attcgcacga cacgaccaga aataatgcaa cttctggtcg tgtgttatgc attacatcgc
    18301 gttcagacgt ttttgtgcct gtttagcgcc atcagtacca gggtatttac tgataacctg
    18361 ctggtatacg gctttcgctt ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt
    18421 aaacatcgca tcagcagcct ttggtgactt cggatagttt ttcactaccg aagcaaaata
    18481 gtacgccgca tcatcttttt tacccttgtt gtagtttaac tgacccagcc aataattggc
    18541 gtttggcagg taagttgaat cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc
    18601 gtcatcctgg cgggatttat cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc
    18661 atcgccgctt ttcaccggcg cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt
    18721 tgatgccgcc gcaccgcttt gatcgctgct ggttgattgc gccgctgcac caccgctgct
    18781 gaggctgtcg atctgcaaca ggatctgctt ttgccgctcc acgacctgat tcagttgata
    18841 ctgattttcc tgaatctgac cacgcaggga atcaatatcg gattgattat cagaaagttg
    18901 ttgctggagt tgggttaaaa gctggctgtg agcattagaa atacgctcaa gttgagtgac
    18961 gcggtcttcg accgagcctg agccgacact actgattggt gcctgagcaa aagcggccca
    19021 gggggccgct ataccaacca gtaacgacag actcaatagt tgatgtctga agttactgct
    19081 catgcaattc tcttagtaaa ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc
    19141 atgacccagt actgcaggtt tttctttacc gtaagaaacg atggagatct ggtctgcaga
    19201 aacgccttta ccctgcaggt acatcttaac ggcgttcgca cgacgttcac ccagggagat
    19261 gttgtattcc ggagtaccac gttcgtccgc gtgaccttct acggtgactt tgtaagacgg
    19321 gttgctacgc aggaagtttg catgtgcatc cagcatttga gcgaagtcag aacggatatc
    19381 gtacttgtcc agatcgaagt aaacgatgtt gttctgctgc agctgttgca tttgcagacg
    19441 agcctgctct tcggaagaca tgttgccgtt gccgccgttc gcatccatac cagtgccggc
    19501 acccagcatg ccttcgctgc cgtcattgct ggcgttcttg ttggaagaac atgccgcaat
    19561 tgccataaca ggcagagcaa tcatcagccc tttcagcact ttgttcagtt gcatttcaat
    19621 gattccttta ctattcaatt aattattatc acagatacgg cgaccaggca gggaatttga
    19681 cctgtccatc agttgccgga agacgcgctt tgaaacgccc atctgtagaa accaaattca
    19741 gcacggatcc catcccctga gaagagctgt agattaccat agtgccatta ggtgccagac
    19801 ttggcgtttc atccaggaac gtggacgaca gaacttgtac gcctcccgtt gccagatctt
    19861 gtttggcaat gtgctgctgc ccaccattgg agctgaccat taccataaat ttaccgtcgc
    19921 tgctgacatc cgcatcctgg ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc
    19981 cgttgatatt cactttataa acctgcggac gaccggcctg gtcagaagta aataccaggt
    20041 tctggctatc cgggaaccag gtcggttcgg tattgttact gcgaccatca gtcacctggc
    20101 ggatctgacc agaagccaaa tccattacgt acaggttcag actaccggtt ttcgacaagg
    20161 cgaatgccag tttgctgccg tctggcgaga atgcaggtgc accgttgtga cgcgggaatg
    20221 aagccacctg acgtacagcg ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc
    20281 tttcgaaggt cacataagcc agtttagaac cgtctggtga ccacgccgga gacatcagcg
    20341 gctgcggtga acggtgaacg acaaactggt tgtaaccgtc atagtcagat acgcgcagtt
    20401 catacgggaa ctgaccgccg ttggtctgaa caacgtaggc aatacgggta cggaacgcac
    20461 ctttaatgcc ggtcagtttt tcaaacactt catcactggc ggtatgacca gcataacgca
    20521 gccactgctt gttcactttg tacgagttct gagcaagtac agtacccggt gcgccgccag
    20581 tgtcaacaag ttgataagca acattgtaag aaccatccgg attcggagtg acctgaccga
    20641 caactacagc gtcaatgccc agtgcggacc atgcagatgg ttgtacttcc tgcgcactac
    20701 ccggctgctg tggcagacga gcgcgatcta acggattaaa tttaccgctg ttacgcaagt
    20761 cagcagcaac gatgccgcca atatcttcag gtgccgcacc aggccccgcc cactggaaag
    20821 gaacaacacc aataggacga ccggaatcta caccgctgtc gatcacaatg cggacttcag
    20881 catgcagaac tgatgcccac agtatgagaa aaccaaatgc tactcgtaat gcctgcttca
    20941 tcatatctcc cttatctgga ccgatggccc acgataattt agcagaatgt taacaaacta
    21001 aaatacacaa aactaccaga accccgtggc aaccggtgcc tgatgttgac cgtccgaaca
    21061 gtcaacatcg cgattacggt ttgaagtcca atggcgcgtt tttgaacact tcatatactg
    21121 cctggcttgg tggtttcggg atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa
    21181 gtgcgggatc gccaccttca ggtttgatat ccagtaacat accatcgggt gccagtttta
    21241 tgcgcagcgt acaggttttg cctgcatagg acgatgcgtc atagaactta ctttcgatag
    21301 cagatttaat ctgcccggca tagttattga tatcggcccc tgatgcgcca ttgtttttag
    21361 tattaccact cccggcaggc gaagcattgt tccctttcgc ccctcccccc gttttcggtg
    21421 cattcttacc agagcttagc tcaccgaaaa tatcatctgc ctctgcggcg gctttttctg
    21481 ccgctgcttt cttatcagct gctgcctttt cagcagccgc tttttctgct gcttttttgt
    21541 cggctgcagc tttcttatca gctgcagcct tttcagcagc cgctttcttc tcagcttctg
    21601 ctttggcttt ttcagcagct tcagttgccg ctttctttct tgcttcagct gcagctgctt
    21661 ctgccgcttc cgctttcttc ttcagtgccg cagcggctgc ctcggctttt ttctgcgctg
    21721 cggctttggc ggcttctgct tctgcttttt tctttgcgtc tgcagccgct ttcttcgctg
    21781 cttcttccgc agctttagca tctgcttcgg ccttcgcttt agcatctgcc gccgctttcg
    21841 ctgccgcctc ttcagcttgc ttctgcttta actcggcctg ttttgcggct tcttcagcct
    21901 gctttttctg ctcctgagcc gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt
    21961 cagccgcttg tttctcacgc agttcttcag cagcctgctg ttccttcatc ttgcgctgtt
    22021 catcagaacg cttcgcgctt gattcctggc tttgcatgcg tttgtactgc tcaactaccg
    22081 cacctgaatc aaccatgaca gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt
    22141 ctatattctc atcgaacgaa ctccagatca gcgccgcaaa taagatgaca tgcagcactg
    22201 ctgaaataat tatcgcccgc ttgagcttgt cgttttgttc ggttgccttt gacactctcg
    22261 gtttccaaaa actgttcgcc tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt
    22321 tagataggct gcgtcattaa accaaccgat ttcacacccg cactatgtaa caagttcagt
    22381 gctttaatta tttcatcgta aggcacatct tttgcgccac cgatcagaaa gaccgttttc
    22441 gggttggcct tgaagcggct ggacacttcc gccaccacct gctctggtgg taaacgctcc
    22501 aggcgatctt tctcaaccac cacggtgtac tgaccaatac cagacacttc aacaatcact
    22561 ggcggattat cgttactgct caccgcctgt gattcagtag cgtctggcag atcgacctcc
    22621 acgctctggg tgatgatggg cgctgtcgcc atgaagatca gcaacagcac cagcagtacg
    22681 tccagcaacg gtacaatgtt gatttcggac ttaagttcgc gacgacctcg tccacgcgct
    22741 ctggccatga cttacccctt attgctctcg ctaacggtaa acgcctggcg gtgcagaatc
    22801 gcggtaaact cttccataaa gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg
    22861 aggcggttgt aggccataac tgccgggata gcggcaaaca gaccaattgc agtcgcaatc
    22921 aacgcttctg cgataccggg cgcaaccatt tgcagtgttg cttgttttac cgccccgagg
    22981 gcgataaagg catgcatgat cccccagacc gtaccaaaca gaccaatata cgggctgatg
    23041 gagccaaccg taccgaggaa cggaatgtgc gtttccagat tttcaagttc acggttcatg
    23101 gagatacgca tagcacgcga cgccccttcc actacggctt ccggcgcatg gctattggca
    23161 cgatgcaggc gcacaaactc tttgaacccg ctgtaaaata tttgttccga accagtcaga
    23221 ttatcccgtt tcccctgact ctcttgatag aggcgagaga gttcgattcc agaccagaat
    23281 ttatcttcaa acgcttcggc ttcgcgcgcc gctgcgttaa gaatacgggt ccgctggata
    23341 ataatggccc aggatgcgat tgaaaaacca atcaaaatca acatgataag tttaaccaga
    23401 aggctagcct tcaggaacaa atcaaggata ttcatgtcag tcactgctta aactccgcga
    23461 caatagactt gggaagcgca cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa
    23521 cctctgcttc attcagcaaa gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag
    23581 aggtgccacg cattgatgtt atttcagtct gtatttcgag catatcgtcg agccgcgcag
    23641 gtgcgtaata ttccaccgtc attttacgta ccacaaaggc aacgcgttca gccatcagcg
    23701 cctgctgact gaagtgatga tgacgcagca tctctgtgcg tgctctttca taaaaagcga
    23761 cgtaactggc gtggtacacc acaccaccgg catcggtatc ttcatagtag acgcgagccg
    23821 gccatcgaaa cagcgttgta ttcactttac atcccggtaa tgcaacaaaa gttagagctt
    23881 ttaaacgctg ctactatacg cgcgcgattg aggtttggga atgagggcaa gttaagggag
    23941 cgtaaaaatt tatgggcctg gagcggccca taatttttta aagagaattc tgatttaaaa
    24001 gaagaagaaa atcagcccga taatgagaac aatatcggca agcaatgggc aaaaaatccc
    24061 ttgccaaaga accttctgcg gacgaaagcc cacgccgtga atcacaccag cacagacggc
    24121 ccacatcagc aataaaccat gccagatttc cagttcactg gtcttcgcgg caaaacgaga
    24181 tgggtcccaa aacatacatc ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag
    24241 ggggcgcttg tccattaccg catacaaggt cgcgataatc ttactcatca gatgtcttct
    24301 tgaccggctt tgcctgattc gacgtgttca agcgccagcg cggtgattac cccaaacgaa
    24361 caggcaagaa gcgttcccag aatccatgcg aaataccaca tttttagctc cttacttagt
    24421 acagagagtg ggtgttacgt tcaatatctt ctttggtgat acgaccgaac attttccagt
    24481 aacaccaggc ggtgtagagc agaatgatcg gtaccagaac caccgcaacc caggtcatga
    24541 cgttaagcgt cagctggctg gaagttgcat cccacattgt cagacttgcg ttcatcatgg
    24601 tgctggacgg catcacaaac gggaacattg cgataccggc tgtcaggatg atgcaggcca
    24661 gcgtcaggga ggagaacaca aacgcccacg cggctttatc catacgtgca gtcaggatgg
    24721 tcagcagcgg cagaaccaca cccagtgccg gaatagccca cagaattggc gtgttgttga
    24781 agttaaccag ccatgcgcca gcttcacgaa ccacttcttt attcagtggg ttagaggctg
    24841 cgtaatggtc cattgtcgat ttcacgacat aaccatcgat accgtacatc acccatacgc
    24901 cagccagtgc gaaacagacc agtgtcacca gcgcagccac ctgagccgtt gcacgggtac
    24961 gcaggtgcag ttcgcccacg gtacgcattt gcagataggt tgcgccctga gtaatgatca
    25021 tccctacgct caccacgcct gccagcaggc cgaacgggtt aagcaactgg aagaagttac
    25081 cggtgtagta cagacgcaga tattcatcaa cgttgaacgg tacgccctgc aacaggttac
    25141 cgaacgcaac accaattacc agcggcggaa cgaagctacc aatgaagatg ccccagtccc
    25201 acatgttacg ccagcgggtt tcttcaatct tggagcggta gtcaaaaccg accggacgga
    25261 agaacaaaga cgccagcacg aggatcatcg ccacatagaa gccggagaac gcagcggcat
    25321 agaccatcgg ccaggcagca aagagtgcgc cgcccgcggt gatcagccaa acctggttac
    25381 cgtcccagtg tggtgcaatg gagttaatca taattcgacg ctcggtgtcg ttacgaccga
    25441 ggaaacgggt gagcatgccc acccccatgt cgaaaccgtc agtgactgca aaaccaatca
    25501 gcagaacgcc aaccagcagc caccagataa aacgcaatac ttcataatcg atcatttgac
    25561 gactcctgtc ttagcgtgcc ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac
    25621 cggttttcag gctgcttggg ccgaggcgtg caaatttgaa cattaagaac aattctgcca
    25681 ccaggaacag ggtatacagg ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg
    25741 tcagtgacga attcgccaca gctgtcggca gcacttcacc gatagcccac ggttggcggc
    25801 catattcagc cacgaaccag cccgcttcta cagcaatcca cggcagcgga ataccgtaca
    25861 gcgcggcgcg cagcagccat tttttctcgc caatgcggtt gcggatgaca ctccagaaag
    25921 agagcgcgat gattgccagc agcaggaagc cacacgccac cataatacgg aacgcgaagt
    25981 acagcggcgc tacacgcgga atggagtctt tagttgcctg ttgaatctgc gcttcagtcg
    26041 catcagccac gtttggcgta tagcgtttca gcagcagacc gtaaccgagg tctttcttca
    26101 tgctattgaa ctggtcacga accgcctggt cggtagaacc agaacgcagt tgttcgagca
    26161 gagagtacgc cttcatcccg ttacgaatgc gttcttcatg ctgcaccatc agctctttca
    26221 ggccgataac cggggtatcc acggaacgcg ttgcaatgat gcccagtgcg taagggatct
    26281 gaatcgcaaa tttgttcgtc tcctcttcct gatcaggaat gccgaacaga gtaaaggcag
    26341 caggcgcagg ttgcgtttcc cactcggctt caatagcagc cagtttggtt ttctgcacgt
    26401 cgcccatttc gtagccggat tcatcaccca gaacaataac agacagaaca gcagccatac
    26461 cgaagctggc agcgatagca aaggagcgtt tagcgaaggc gaagtcacga cctttcagca
    26521 tataccatgc gctgataccg aggatgaaca tcgcgccagt cacataacca gacgctacag
    26581 tgtgaacgaa tttcacctga gcaaccgggt taagcaccag ctcggagaag ctcaccatct
    26641 ccatacgcat agtttcaaag ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg
    26701 caaccagaat ccacagtgcg gacaggtttg aaccgagcgc caccagccag gtgacacaca
    26761 tatgctgaac tttacccaga cgatcccaac cgaagaagaa cagacctaca aaggtggatt
    26821 cgaggaagaa ggccatcaga ccttcgattg ccagcggcgc accgaagata tcccctacat
    26881 agtgggaata gtaagaccag ttagtcccga actggaactc catggtcaga ccggtagcca
    26941 cacccagagc gaagttgata ccaaacaact tgccccagaa cttggtcata tctttataaa
    27001 tctgtttgcc ggagaggacg tagaccgttt ccataatggc cagcaggaac gccataccga
    27061 gcgtcagtgg cacaaaaagg aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca
    27121 gttcgactat atctaacatc atgactcctt gctcatcgca tgaagactcc gagagtgaac
    27181 cccgttagaa agggtcacac gcatgcccca atagaaattt gtttgaacac tttcatcata
    27241 ccatgtgaat gcacacaaaa acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc
    27301 aaattgatcc cacgtatata ttacgccgca aaatccttac aataaacagg tttttgttga
    27361 agcaaatttg catttttcga cggtgatcaa tttatagcta aattaccgcc tttcagccaa
    27421 tttgatcgag aacaatttat ctctttttga tgcccatttc caagacttat acattgataa
    27481 atatcaattc ctacaaatta tgttaatcat gtttcaataa ggtaacttta gtgacattta
    27541 tgtttaaaat gtgtgagtta taaggctgaa attagtttcg gttggcaatt aaagataaaa
    27601 agagacgaag caaaaacagc acggttgacc ttaattcttt ttttggtcac tggctaatac
    27661 gacgcagagt ttcaggatta ctaataaagg cataaatcca cccccgccga tattaacatt
    27721 taattaactt ataattatat tataattaac tcacccaggg ttattatttt gttaaaaata
    27781 aagccacata tacgtggctt gagagtgaag ataaacgcaa aattacctga tgcgctacgc
    27841 ttgccagtgt ctacatcatc tctgcaatat attgaatttg cctgcttttg tagaccgaat
    27901 aaagcgttca tgccgtatcc ggcatgaaca aagcgccctt tgtcaacaat ctggccgcgc
    27961 atatgtggct tcggtttgat tgctattcag gcaagccggt aactgaacgt ccgtgactgg
    28021 ccgggtaaaa aaggccccga tagatttgaa ccttgatttt cctcggtggt aatgtgttca
    28081 tccatcatcg tttctgagca agaaatcacc tcgcgactga aagcaacagt cgcatcacag
    28141 gttgctgatt cagccggatt aaacagccgt aaaatcactt cttgtcggtc ttcagctttc
    28201 ttaagtgcgc ttatcaggca tcccactggg ggcattttca acaaactata actttccggc
    28261 acgttgaatc cggctttgtt gagcttcatc gcatcccatg ggattttgtt gtaacactgt
    28321 actggagtca gccatgctcg cgcctgctga gctacaccag cggcggttgg cgtaccggta
    28381 taactcaata aacttaggcg acaagaaagc agaccacgta gttgtgagtc cgggactggc
    28441 attttaattc ccgaaggccg cccaggcctt aaaagcagat cttctttgcc cagtaagccc
    28501 acgccacgca gcaacgtaat ggcaaaggtt ttcttctctt caccgatgac ttcaaattca
    28561 cgtaaccctt cgctaaagac agccatgccg ttacgccctt cctgtaaggc aacatagttg
    28621 agcatattcc ataccggaac cggcgcttct ttccagcctt cttgctgcca gttgttcatt
    28681 gcactgtcgt tcacggggcg cgttagcgaa ccaaactgcg tatctgccag aacactgtcg
    28741 gtgttaaaag gtgtagggac caggacacga aggcgatgat cgtcagcctg gttatcaagg
    28801 ttgatatcca catcaatacg cctgctgtta tgactaagag tgacaaccaa caccacccct
    28861 actctgccag tggattgccg ggcgctgcgt tctgacaaat tgagcggcac tgccatgtca
    28921 tagcggataa cagccctgct ctgccaggct tcatgaataa tatcgcattg cggtttcgcg
    28981 ttcgctgcgg taattaccca ctcttctttt gcgggtgaat agtcatattc atcaccatca
    29041 tcagagct
//
LOCUS       DAJTOR010000034        28242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTOR010000034 DAJTOR010000000
VERSION     DAJTOR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.034"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..322)
                     /locus_tag="OO249_001346"
     CDS             complement(<1..322)
                     /locus_tag="OO249_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5635701.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIITILVVGSIWIMWNL
                     NYNMM"
     gene            complement(322..936)
                     /locus_tag="OO249_001347"
     CDS             complement(322..936)
                     /locus_tag="OO249_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5635702.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(926..2917)
                     /gene="cyoB"
                     /locus_tag="OO249_001348"
     CDS             complement(926..2917)
                     /gene="cyoB"
                     /locus_tag="OO249_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR5635703.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWANSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(2939..3886)
                     /gene="cyoA"
                     /locus_tag="OO249_001349"
     CDS             complement(2939..3886)
                     /gene="cyoA"
                     /locus_tag="OO249_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCR5635704.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(4347..5822)
                     /gene="ampG"
                     /locus_tag="OO249_001350"
     CDS             complement(4347..5822)
                     /gene="ampG"
                     /locus_tag="OO249_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5635705.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(5866..6444)
                     /locus_tag="OO249_001351"
     CDS             complement(5866..6444)
                     /locus_tag="OO249_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5635706.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLAASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            6749..7066
                     /gene="bolA"
                     /locus_tag="OO249_001352"
     CDS             6749..7066
                     /gene="bolA"
                     /locus_tag="OO249_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5635707.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            7410..8708
                     /gene="tig"
                     /locus_tag="OO249_001353"
     CDS             7410..8708
                     /gene="tig"
                     /locus_tag="OO249_001353"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5635708.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDSAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            8953..9576
                     /gene="clpP"
                     /locus_tag="OO249_001354"
     CDS             8953..9576
                     /gene="clpP"
                     /locus_tag="OO249_001354"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5635709.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            9702..10976
                     /gene="clpX"
                     /locus_tag="OO249_001355"
     CDS             9702..10976
                     /gene="clpX"
                     /locus_tag="OO249_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5635710.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            11164..13518
                     /gene="lon"
                     /locus_tag="OO249_001356"
     CDS             11164..13518
                     /gene="lon"
                     /locus_tag="OO249_001356"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5635711.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            13727..13999
                     /gene="hupB"
                     /locus_tag="OO249_001357"
     CDS             13727..13999
                     /gene="hupB"
                     /locus_tag="OO249_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5635712.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            14191..16062
                     /gene="ppiD"
                     /locus_tag="OO249_001358"
     CDS             14191..16062
                     /gene="ppiD"
                     /locus_tag="OO249_001358"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5635713.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            16213..16584
                     /locus_tag="OO249_001359"
     CDS             16213..16584
                     /locus_tag="OO249_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5635714.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            16678..17076
                     /gene="fadM"
                     /locus_tag="OO249_001360"
     CDS             16678..17076
                     /gene="fadM"
                     /locus_tag="OO249_001360"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5635715.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(17128..17823)
                     /gene="queC"
                     /locus_tag="OO249_001361"
     CDS             complement(17128..17823)
                     /gene="queC"
                     /locus_tag="OO249_001361"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5635716.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(17888..19588)
                     /locus_tag="OO249_001362"
     CDS             complement(17888..19588)
                     /locus_tag="OO249_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR5635717.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLALG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            19688..20470
                     /gene="cof"
                     /locus_tag="OO249_001363"
                     /pseudo
     CDS             19688..20470
                     /gene="cof"
                     /locus_tag="OO249_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
     gene            20659..21117
                     /gene="decR"
                     /locus_tag="OO249_001364"
     CDS             20659..21117
                     /gene="decR"
                     /locus_tag="OO249_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5635718.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            21147..22919
                     /locus_tag="OO249_001365"
     CDS             21147..22919
                     /locus_tag="OO249_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5635719.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYFLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASENIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            22912..24693
                     /locus_tag="OO249_001366"
     CDS             22912..24693
                     /locus_tag="OO249_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5635720.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            24874..25212
                     /gene="glnK"
                     /locus_tag="OO249_001367"
     CDS             24874..25212
                     /gene="glnK"
                     /locus_tag="OO249_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5635721.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            25242..26528
                     /gene="amtB"
                     /locus_tag="OO249_001368"
     CDS             25242..26528
                     /gene="amtB"
                     /locus_tag="OO249_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5635722.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(26577..27437)
                     /gene="tesB"
                     /locus_tag="OO249_001369"
     CDS             complement(26577..27437)
                     /gene="tesB"
                     /locus_tag="OO249_001369"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5635723.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            27655..28227
                     /locus_tag="OO249_001370"
     CDS             27655..28227
                     /locus_tag="OO249_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5635724.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
BASE COUNT         7110 a         6883 c         7688 g         6561 t
ORIGIN      
        1 tcatcatgtt gtagttgagg ttccacataa tccagatgga gcctacaacc aggatagtga
       61 tgattagcac ggtgaagaca aacgccgtca tgttccagcc ttcatctgat ttggtattca
      121 tgtgcaggaa gcacaccaga tgcaccagaa cctgtaccac tgccattgcc aggattgttc
      181 ccagaattac ggccggagag gcagcccctg tcatcaccat ccagaacgga atcaccgtca
      241 gaatgatcga caggataaag cctgtcatgt aggtttttac gctgccatgg gacgcgccgc
      301 tgtgatcggt agaatgactc attacatcgc ccccatcaga taaacaacag tgaacacaca
      361 gatccaaacc acatccagga agtgccagaa caggctcagg cacatgatgc gggtacggtt
      421 agtgctggtc aggccgcgac gagcgatttg taccatcagc accgccatcc agataagacc
      481 ggaagtgacg tgcagaccgt gcgtgccgac cagcgcaaag aacgctgaca ggaagccgct
      541 gcgatccgga cccatgccgt taacaatcag gtgatggaat tcgtagattt ccatcccgat
      601 aaatccggca ccaaacaaac aggtcaacgc cagccaggag ataacctggc ttttgttgtt
      661 tttgtacatg gcgatagccg ccatgccgta ggtgatggag ctgaacaaca gcaagaaagt
      721 ttcaaccaga acgaacggca gttcgaaaat gtccttacct gtcgggccgc ctgcggtgcc
      781 gttcaccaga acggcatagg tagcaaacaa gattgagaac agaatgcagt cgctcatcag
      841 gtagatccaa aatccgaaga ttttggttcc gcctgcatcg tggtgcccgt gttcgtgcgc
      901 gtgggcagtc gcgtgcgtca aagtatcagt tgccattttt cagccctgcc ttagtaatct
      961 catcgaaatg ctggttttcc agtttttcga tttctgccac cggcacgtag taatccacgt
     1021 cctcgtcgaa gcttttcacg atccaggtga tgatcatgcc tgcgaagcca acaatcgcca
     1081 gccaccagat atgccagatc atggcgaaac cgaagatggt ggagaaagct gcaatgacga
     1141 tacctgcacc gctgtttttc ggcatatgaa tttcttcata gtggtcaggc tttttatacg
     1201 cttcgccttt ctctttcatt tcccagaatg catcacgctc gtgaacgtgc ggcacaacgg
     1261 caaagttata gaacggaggc ggggaagagt ttgcccactc cagcgtacgg ccaccccacg
     1321 ggtcgccagt caggtcacgg ttctggtcgc ggtcgcgaat agaaacgtac atctgaataa
     1381 cgaggcagag aatacccagc gcaatcagta ctgcaccgct agctgcaatc atcagcatgg
     1441 tgtggaactg cgggtcaatc tgctggctca aacgacgggt catgcccatg aagcccagcg
     1501 catacagcgg cataaaggca acgaagaagc cgatgatcca gaaccagaac gcgcgtttac
     1561 cccaggtttc attcagtttg aaaccgaacg ctttaggcca ccagtaggtc atccctgcga
     1621 agcagccgaa gaccacgccg ccgatgataa cgttatggaa gtgcgcaatc aggaacaggc
     1681 tgttatgcag aacgaagtcc gcgcccggta ctgccagcag cacgccagtc atcccgccca
     1741 ccgagaaagt gacgataaaa ccgatggtcc acagcatcgc agaatggaac acgatacggc
     1801 cctgatacat ggtgaacagc cagttgaaga tcttcacccc ggtcgggatg gcgataatca
     1861 ttgtggtgat accaaagaag gcgtttacgt tcgcgcccgc acccatcgta aagaagtggt
     1921 gcagccaaac gatgaacgac agcacggtga tacagacggt tgcccatacc agcgaggtat
     1981 aaccaaacag acgtttacgc gagaaggttg ccgcaatttc ggagaacaca ccgaaaacag
     2041 gcaggatcag gatgtaaact tccgggtggc cccaggccca aatcaggttg atgtacatca
     2101 tcatgttgcc acccatatcg ttggtaaaga aatgggtgcc cagatagcga tccagggtca
     2161 acaacgcgac ggtaaccgtc agaattggga aggaagcaat aatcagtacg ttcgcgcaca
     2221 gtgatgccca ggtaaatact ggcatcttga acatggtcat gcccggtgcg cgcatcttca
     2281 gaatggtaac gaagaagttg ataccggtaa gcgtcgtacc tataccggat agctggagac
     2341 tccatatcca gtaatcaaca ccgactcccg gactgtactc tattcccgat agcggtggat
     2401 aggccagcca gccggtctgc gcaaattcgc ccacgccgag agaaacgtta accagaatca
     2461 caccaacaac agtaaaccag aagcttaagt tgttgaggaa cgggaacgca acgtcacgcg
     2521 cgccgatctg cagcggaacc accaggttca tcagaccgat aacgaaaggc atcgccacga
     2581 agaagatcat aatcacgccg tgcgcggtga agatctgatc gtagtggtga ggtggcagga
     2641 agcccgcttc gcccgccgag gcaagagcct gctggctacg catcataatg gcgtcagcaa
     2701 aaccacgcag caacatcaca atcgccacga tgatatacat gataccgagg cgtttatggt
     2761 cgacggaggt cagccactct ttccacaggt aggtccactt accgaagtaa gtgatcaggc
     2821 caacgagcgc cagacctccc aaaataatgc cagcgatcgt aaccatgacg ataggttcat
     2881 ggaacgggac tgcatcaagt gataattttc cgaacatctt tattcttcct caaccccttt
     2941 aatgggcgga ttccgcgtgg ctcatgtcca tgccttccat accttcgtgt gcgctgtgct
     3001 caccttctgg ctgggtcatg tccatgctct taccgtgagc cataaacttg ttaatcacat
     3061 cagcaaacaa gtctggtttc acgttggaga aatattccac ctggttgtat tcgctaggcg
     3121 cggccagttt ttcgaacgca gccatgtcag acatggtgtt cggcgactgc ttcgcttttg
     3181 cgacccactg gtcgaattcg gcgcgatccg gtgttgcaat agctttgaac ttcatgcctg
     3241 agaagcccgg gccgctgtag ctggcggaga taccgtcata agtaccgggt tcgttggcga
     3301 tcagatgcag gcgagtctgc ataccagcca tggcataaat ctggctaccc agacgcggaa
     3361 tgaagaagga gttcatcacg gagttggagg tcactttgaa gtacaccgga gtgttcgccg
     3421 ggaaagcgat ttcattcacg gtagcaatgc cctgttccgg gtagatgaag aaccatttcc
     3481 agtccatgga aaccacttcg atggtaatgg gcttctcgtc gtgtgccagc ggcttgctag
     3541 gctcaagagc gtgagtggtt ttccaggtca gtaccgcaag gaagatgatg attaagatag
     3601 gtaccgtcca gaccacagct tccactttat tggagtgtga ccagttcggg ctgtacttag
     3661 catctttatt gctcgcacga tacttccagg cgaaaccaac agccatcaag attgcgggaa
     3721 taacgacaat caacatcagg ccaaatgccg tcagtatcag tgaacgttgc tccagaccaa
     3781 tctgtccttt gggatctaac agcgcagaat tacagccact gagcaataca gtgcctgcaa
     3841 ataatgacaa ccatcccaaa cttttattgt atttcctgag tctcatttaa cgacctcaat
     3901 tccacgggat ctggtggcgt ttaaagtgtg tgggcatttt atgggaaggt tacattactg
     3961 taaacatgat taaattcctg ttacttggat ttgattgtca ggtcacatat gttgcaaaac
     4021 atatcaagtt gattttaaga agctgttgca aaagggcgtt ataacgaaag gggagtgcag
     4081 aaggagtttg gatgggcaat tggtataacc aatctggaat aaaataattg tttaacaaat
     4141 aattatcatt atggtgacaa ttgggtgggg ggaaaataaa gactcgttag ctgaatcgtg
     4201 taagaaaaaa tagaattcat aaattgcgtc aataatttcc aaacgctaag ccgcacaaaa
     4261 gaacaaatat tattaagcga tactgatgtt tatttagccg taataattac ggcgagtgat
     4321 tactacagct aaataatatt tacagattac gtcagatgcg tttttcgtag agccagataa
     4381 tccagcaaac caccaagcac gacgccagaa agggcgacta aaaccccgac ttccagcaga
     4441 gcaggcagga aagagaagtg cgtcaaatcc agcgcgtcca tcgtcaacag cagtaaccac
     4501 acggccaaca ggctgacgcc cgccgccagt gtccacatgg caaaggcata acctgccgga
     4561 tattcggtac gggagataaa gttgtcattt actcgtgtat attcaagcgt ctggcggcaa
     4621 accagcagca aaataagccc cggtacggca gcggcgacgg agaatagata gaatgtcgac
     4681 cagccgtgtg cttcaacaaa ccaacccgcc acggggccga cataaactcg tcctacagcg
     4741 gaaagcgctg agagcagggc aaattgagta gcggaaaatg acttattaca cagcgtcatt
     4801 aacagcgcga caaaggctga tgtgcccatc ccgccacaga ggttttcgaa aaagacggct
     4861 gcgcccatgc tgtagagatg cttatcagta atcgacagca gccagtaacc agcgttagac
     4921 gcaccttgta aaatgccgaa aatcagcagc gcccggaaca gcgacaggcg ctgcatcaaa
     4981 atcccaccgt acaatgcgcc cacaatggtc gccagtaagc caagcgtttt gttaaccacg
     5041 cctacttcac ccgcatcaaa cccgacgccg cgaatcaaaa acgtggttgt caggctcatg
     5101 gcgaatgcgt cgcccagctt atacagcacg ataagaagca aaataagcca ggcattattg
     5161 cgaccaaaga aatctcgcag aggtgcaaca accgcttgtt ccagcgtttt tggcacagga
     5221 atggtgtcgg tcggttctgg tgcaagcaac gtcgcgataa tacaagggat caacagcgcc
     5281 gccatcaacc agtacatgcc ctgccagccc agccatttat ctgccagcca cagggctaag
     5341 ccgccggaaa ccagcatccc taaacggtaa cccagcacgc tgattgccgc gcccgcacca
     5401 cgttcttctg ccgggagcac atcggttttc cacgcatcga agacaatatc ctgggaggcc
     5461 gagcaaaaag cgatcaccac cgccagtgcc gccatccagc ggagttgggt gccaggttcg
     5521 agaaaaccca tcgccgcaat ggcgactaat aacaggattt gcgtggcgag cagccaaccg
     5581 cgccgccgcc caaaaaatgg gggcgtgtag cggtccatca gcggtgacca gaggaattta
     5641 aaaacgtaag cctggcctac cagagagaag aaaccaatgg ttttgagatc aatattctcg
     5701 accgtcatcc aagcctgtaa ggtaccggat gtcagggcga gcggtagccc ggaagcaaaa
     5761 cccaggatca gcaatatggc tgaacgcggc tgttgaaaaa tacgtaaata ttgactggac
     5821 atgggcgtct acaggcccgg tttgcaccgg gccagagggc agaaattaac gcgcgttctg
     5881 cttgatgaat tcgtgaatgc tggtgtcctg agacatatca gcgatggtat cggtcagcac
     5941 actgttaacc gcatcggcga tatttttgtt ggaggcctgg aacgcacctt caacgttgta
     6001 gctggcacga tagtttttgg ttattttgtt gccattctgc gcggtagcga tgatggcgat
     6061 atccgctttg gtagcgatgt tgtagcgcac gttgccctgg gacacgtcag catacagttg
     6121 gctaacgatg atttgcagat taaccgggcc attcggacca accatgtaac cacgcgcggt
     6181 catctgtttt tccagcactt cttgcagcag gaaacgcaga tcgcgggagg cggccagggt
     6241 aacgatttga ttatcgcggg tgacttttgc cagcgcctga tcggtacgct gatcggcacc
     6301 attaatgctt acggtgacgc ccatcaggct tggatcctgc tgtggcagtg taatcgtcgg
     6361 ggaaacttca atagttgttg gcggttttgc gcatcctgca agcataaaca gagcaactaa
     6421 cgggaagagg atttttttga acatgttcgg gctctcagag actcttaagc gtgtttggta
     6481 aaaattcccg ccatcataac attgccaacg gcgaggggaa gtgggtaagg catgtaaatt
     6541 catcatgttg acgaaataat cacccctggt aaaagaaacg ctgatgcgag gcctgtgttt
     6601 caatctttaa atcagtaaac ttcatacgct tgacggaaaa accaggacga aacctaaata
     6661 tttgttgtta agctgcaatg gaaacggtaa aagcggctag tatttaaagg gatggatgac
     6721 atctcagcgt tgtcggagga gatatttcat gatgatacgt gagcggatag aagaaaaatt
     6781 aagggcggcg ttccaacccg tattcctcga agtagtggat gaaagctatc gtcacaatgt
     6841 cccagccggc tctgaaagcc attttaaagt tgtgctggtc agcgatcgtt ttacgggtga
     6901 acgttttctg aatcgtcatc gaatgattta cagtacttta gcggaggaac tctctactac
     6961 cgttcatgcg ctggctctgc atacttacac tattaaggag tgggaagggt tgcaggacac
     7021 cgtctttgcc tctcctccct gtcgtggagc aggaagcatc gcgtaaaaac gcatttgcaa
     7081 ctgtcggcgc tttttcagta tgttgctaaa gattttatga aaaacggcct gcgggccgtt
     7141 ttgttttgtc tggattttgc gctttttgcc cagcattcag acgaaaattg cccgggaatt
     7201 gtgaaaaaat acgcgacagc gcgcaataac cgttctcgac tcataaaagt gatgccgcta
     7261 taatgccgcg tcctatttga atgctttcgg gatgattctg gtaacaggga atgtgattga
     7321 ttataagaac atcccggttc cgcgaagcca acaacctgtg cttgcggggt aagagttgac
     7381 cgagcactgt gattttttga ggtaacaaga tgcaagtttc agttgaaacc actcaaggcc
     7441 ttggccgccg tgtaacgatt actatcgctg ctgacagcat cgagaccgct gttaaaagcg
     7501 agctggtcaa cgttgcgaaa aaagtacgta ttgacggctt ccgcaaaggc aaagtgccaa
     7561 tgaatatcgt tgctcagcgt tatggcgcgt ctgtacgcca ggacgttctg ggtgacctga
     7621 tgagccgtaa cttcattgac gccatcatta aagaaaaaat caatccggct ggcgcaccga
     7681 cttatgttcc gggcgaatac aagctgggtg aagacttcac ttactctgta gagtttgaag
     7741 tttatccgga agttgaactg cagggtctgg aagcgatcga agttgaaaaa ccgatcgttg
     7801 aagtgaccga cgctgacgtt gacggcatgc tggatactct gcgtaaacag caggcgacct
     7861 ggaaagaaaa agacagcgct gttgaagcag aagaccgcgt aaccatcgac ttcaccggtt
     7921 ctgtagacag cgaagagttc gaaggcggta aagcgtctga tttcgtactg gcgatgggcc
     7981 agggtcgtat gatcccgggc tttgaagacg gtatcaaagg ccacaaagct ggcgaagagt
     8041 tcaccatcga cgtgaccttc ccggaagaat accacgcaga aaacctgaaa ggtaaagcag
     8101 cgaaattcgc tatcaacctg aagaaagttg aagagcgtga actgccggaa ctgactgcag
     8161 aattcatcaa acgtttcggc gttgaagatg gttccgtaga aggtctgcgc gctgaagtgc
     8221 gtaaaaacat ggagcgcgag ctgaagagcg ccatccgtaa ccgcgttaag tctcaggcga
     8281 tcgaaggtct ggtaaaagct aacgacatcg acgtaccggc tgcgctgatc gacagcgaaa
     8341 tcgacgttct gcgtcgccag gctgcacagc gtttcggtgg caacgaaaaa caagctctgg
     8401 aactgccgcg cgaactgttc gaagaacagg ctaaacgccg cgtagttgtt ggcctgctgc
     8461 tgggcgaagt tatccgcacc aacgagctga aagctgacga agagcgcgtg aaaggcctga
     8521 tcgaagagat ggcttctgcg tacgaagatc cgaaagaagt tatcgagttc tacagcaaaa
     8581 acaaagaact gatggacaac atgcgcaatg ttgctctgga agaacaggct gttgaagctg
     8641 tactggcgaa agcgaaagtg actgaaaaag aaaccacttt caacgagctg atgaaccagc
     8701 aggcgtaatt tacgcagcat aacgcgctaa attcgcacaa aggcccgtca ccgccaggtg
     8761 gtgggctttt tttgtcatga attttgcatg gaaccgtgcg aaaagcctct ttcggtgtta
     8821 gcgtaacaac aaaagattgt tatgcttgaa atatggtgat gccgtaccca taacacaggg
     8881 actagctgat aatccgtcca taaggttaca atcggtacag caggtttttt caattttatc
     8941 caggagacgg aaatgtcata cagcggcgaa cgagataact ttgcacccca tatggcgctg
     9001 gtgccgatgg tcattgaaca gacctcacgc ggtgagcgct cttttgatat ctattctcgt
     9061 ctacttaagg aacgcgtcat ttttctgact ggccaggttg aagaccacat ggctaacctg
     9121 attgtggcgc agatgctgtt cctggaagcg gaaaacccag aaaaagatat ctatctgtac
     9181 attaactccc caggcggggt gatcactgcc gggatgtcta tctatgacac catgcagttt
     9241 atcaagcctg atgtcagcac catctgtatg ggccaggcgg cctcgatggg cgctttcttg
     9301 ctgaccgcag gggcaaaagg taaacgtttt tgcctgccga attcgcgcgt gatgattcac
     9361 caaccgttgg gcggctacca gggccaggcg accgatatcg aaattcatgc ccgtgaaatt
     9421 ctgaaagtta aagggcgcat gaatgaactt atggcgcttc atacgggtca atcattagaa
     9481 cagattgaac gtgataccga gcgcgatcgc ttcctttccg cccctgaagc ggtggaatac
     9541 ggtctggtcg attcgattct gacccatcgt aattgatgcc agaggcgcaa ctgtgccgct
     9601 atacttatcc agggcggcac aacgctgtaa gcggcttgcg cctgagaatg gcatttgcgt
     9661 cgtcgtgtgc ggcacaaaga acaaagaaga ggttttgacc catgacagat aaacgcaaag
     9721 atggctcagg caaattgctg tattgctctt tttgcggcaa aagccagcat gaagtgcgca
     9781 agctgattgc cggtccatcc gtgtatatct gcgacgaatg tgttgattta tgtaacgaca
     9841 tcattcgcga agagattaaa gaagttgcac cgcatcgtga acgcagtgcg ctaccgacgc
     9901 cgcatgaaat tcgcaaccac ctggacgatt acgttatcgg ccaggaacag gcgaaaaaag
     9961 tgctggcggt cgcggtatac aaccattaca aacgtctgcg caacggcgat accagcaatg
    10021 gtgtcgagtt gggcaaaagt aacattctgc tgatcggtcc gaccggttcc ggtaaaacgc
    10081 tgctggctga aacgctggcg cgcctgctgg atgttccgtt caccatggcc gacgcgacta
    10141 cactgaccga agccggttat gtgggtgaag acgttgaaaa catcattcag aagctgttgc
    10201 agaaatgcga ctacgatgtc cagaaagcac agcgtggtat tgtctacatc gatgaaatcg
    10261 acaagatttc tcgtaagtcg gacaacccgt ctattacccg tgacgtttcc ggtgaaggcg
    10321 tacagcaggc actgttgaaa ctgatcgaag gtactgttgc tgccgttcca ccgcagggcg
    10381 ggcgtaagca tccgcagcag gaatttttgc aggttgatac ctccaagatc ctgtttattt
    10441 gtggcggtgc gtttgccggt ctggataaag tgatttccca ccgtgtagaa accggctccg
    10501 gcattggttt tggcgcgacg gtaaaagcga agtccgacaa agcaagcgaa ggcgagctgc
    10561 tggcgcaggt tgaaccggaa gatctgatca agtttggtct tatccctgag tttattggtc
    10621 gtctgccggt tgtcgcaacg ttgaatgaac tgagcgaaga agctctgatt cagatcctca
    10681 aagagccgaa aaacgccctg accaagcagt atcaggcgct gtttaatctg gaaggcgtgg
    10741 atctggaatt ccgtgacgag gcgctggatg ctatcgctaa gaaagcgatg gcgcgtaaaa
    10801 ccggtgcccg tggcctgcgt tccatcgtag aagccgcact gctcgatacc atgtacgatc
    10861 tgccgtccat ggaagatgtc gaaaaagtgg ttatcgacga gtcggtaatt gatggtcaaa
    10921 gcaaaccgtt gctgatttat ggtaagccgg aagcgcaaca ggcatctggt gaataattaa
    10981 ccattcccat acaattagtt aaccaaaaag gggggatttt atctcccctt taatttttcc
    11041 tctattctcg gcgttgaatg tgggggaaac atccccatat attgacgtac atgttaatag
    11101 atggcgtgaa gcacagtcgt gtcatctgat tacctggcgg aaattaaact aagagagagc
    11161 tctatgaatc ctgagcgttc tgaacgcatt gaaatccccg tattgccgct gcgcgatgtg
    11221 gtggtttatc cgcacatggt cattccctta tttgtcgggc gggaaaaatc tatccgttgt
    11281 ctggaagcgg cgatggacca tgataaaaaa attatgctgg tcgcgcagaa agaagcttca
    11341 acggatgagc cgggtgtaaa cgatcttttc accgtcggga ccgtggcctc tatattgcag
    11401 atgctgaaac tgcctgacgg caccgtcaaa gtgctggtcg aggggttaca gcgcgcgcgt
    11461 atttctgcgc tctctgacaa tggcgaacac ttttctgcga aggcggagta tctggagtcg
    11521 ccgaccattg atgagcggga acaggaagtg ctggtgcgta ctgcaatcag ccagttcgaa
    11581 ggctacatca agctgaacaa aaaaatccca ccagaagtgc tgacgtcgct gaatagcatc
    11641 gacgatccgg cgcgtctggc ggataccatt gctgcacata tgccactgaa actggctgac
    11701 aaacagtccg ttctggagat gtccgacgtt aacgaacgtc tggaatatct gatggcaatg
    11761 atggaatcgg aaatcgatct gctgcaggtt gagaaacgca ttcgcaaccg cgttaaaaag
    11821 cagatggaga aatcccagcg tgagtactat ctgaacgagc aaatgaaagc tattcagaaa
    11881 gaactcggtg aaatggacga cgcgccggac gaaaacgaag ccctgaagcg caaaatcgac
    11941 gcggcgaaga tgccgaaaga ggcaaaagag aaagcggaag cagagttgca gaagctgaaa
    12001 atgatgtctc cgatgtcggc agaagcgacc gtagtgcgtg gttatatcga ctggatggta
    12061 caggtaccgt ggaatgcgcg cagcaaggtc aaaaaagacc tgcgtcaggc gcaggaaatc
    12121 cttgataccg accattatgg tctggagcgc gtgaaagatc gcatccttga gtaccttgcg
    12181 gttcaaagcc gtgtcaacaa aatcaaggga ccgatcctct gcctggtagg gccgccgggg
    12241 gtaggtaaaa cctctcttgg tcagtccatt gccaaagcca ccgggcgtaa atatgtccgt
    12301 atggcgctgg gcggcgtgcg tgatgaagcg gaaatccgtg gtcaccgccg tacttacatc
    12361 ggttctatgc cgggtaaact gatccagaaa atggcgaaag tgggcgtgaa aaacccgctg
    12421 ttcctgctcg atgagatcga caaaatgtct tctgacatgc gaggcgatcc ggcctctgca
    12481 ctgcttgaag tgctggatcc agagcagaac gtagcgttca gcgaccacta cctggaagtg
    12541 gattacgatc tcagcgacgt gatgtttgtc gcgacgtcga actccatgaa cattccggca
    12601 ccgctgctgg atcgtatgga agtgattcgc ctctccggtt ataccgaaga tgaaaaactg
    12661 aacatcgcca aacgtcacct gctgccgaag cagattgaac gtaatgcact gaaaaaaggt
    12721 gagctgaccg tcgacgatag cgccattatc ggcattattc gttactacac ccgtgaggcg
    12781 ggcgtgcgtg gtctggagcg tgaaatctcc aaactgtgcc gcaaagcggt taagcagtta
    12841 ctgctcgata agtcattaaa acatatcgaa attaacggcg ataacctgca tgactacctc
    12901 ggtgttcagc gtttcgacta tggtcgcgct gataacgaaa actgtgtcgg tcaggtaacc
    12961 ggtctggcgt ggacggaagt gggcggtgac ttgctgacca ttgaaaccgc atgtgttccg
    13021 ggtaaaggca aactgaccta taccggttcg ctcggcgaag tgatgcagga gtctattcag
    13081 gcggcgttaa cggtggttcg tgcgcgtgcg gaaaaactgg ggatcaaccc tgatttttac
    13141 gaaaaacgtg acatccacgt ccacgtaccg gaaggtgcga cgccgaaaga tggtccgagt
    13201 gccggtattg ctatgtgcac cgcgctggtt tcttgcctga ccggtaaccc ggttcgtgcc
    13261 gatgtggcaa tgaccggtga gatcactctg cgtggtcagg tactgccgat cggtggtttg
    13321 aaagaaaaac tcctggcagc gcatcgcggc gggattaaaa cagtgctaat tccgttcgaa
    13381 aataaacgcg atctggaaga gattcctgac aacgtaattg ccgatctgga cattcatcct
    13441 gtgaagcgca ttgaggaagt tctgactctg gcgctgcaaa atgaaccgtc tggcatgcag
    13501 gttgtgactg caaaatagtg acctcgcgca aaatgcacta ataaaaacag ggctggcagg
    13561 ctaattcggg cttgccagcc tttttttgtc tcgctaagtt agatggcgga tcgggcttgc
    13621 ccttattaag gggtgttgta aggggatggc tggcctgata taactgctgc gcgttcgtac
    13681 cttgaaggat tcaagtgcga tataaattat aaagaggaag agaagagtga ataaatctca
    13741 attgatcgac aagattgctg caggggctga tatctctaaa gctgcggctg gccgtgcgtt
    13801 agatgctatt attgcttccg taactgaatc tctgaaagaa ggggatgatg tagcactggt
    13861 aggttttggt acttttgccg ttaaagagcg tgctgcccgt actggccgca acccgcagac
    13921 cggtaaagag atcaccatcg ctgctgctaa agtaccgagc ttccgtgcag gtaaagcact
    13981 gaaagacgcg gtaaactaag cgttgtcccc ggtggggatg tgacgaagtt caagggcgca
    14041 tctactgatg tgcctttttt atttgtattc ggtgactttc tgcgtcttgt gggctgacaa
    14101 ttgcccccgt ttcttgtcac aataggcctt tgcgcgcatc gatacgttgc gtgaggtaca
    14161 cagtcatcta cagcggagtg ttgttacacc atgatggaca gcttacgcac ggctgcaaac
    14221 agtctcgtgc tcaagattat tttcggtatc attatcgtgt cgttcatatt gaccggcgtg
    14281 agtggttacc tgattggcgg aggcaataac tacgccgcaa aagtgaatga ccaggaaatc
    14341 agccgtgggc agttcgagaa cgcctttaac agcgagcgta atcgcatgca gcaacagctg
    14401 ggcgatcaat actccgagct ggcagcgaat gaaggctata tgaaaaccct gcgtcaacag
    14461 gtgctgaatc gtctgatcga cgaggcgctg ttggatcagt acgctcgtga gctgaaactg
    14521 ggtatcagtg atgagcaggt taaacaggcg attttcgcga ccccagcctt ccaggttgac
    14581 ggcaaatttg ataacagccg ctataacggt atcctcaacc agatggggat gaccgccgat
    14641 cagtacgccc aggcgctgcg taaccagctc actacccaac agctgattaa cggtgttgcc
    14701 ggtaccgatt ttatgctgaa aggtgaaacc gacgagctgg cggcactggt cgcgcaacaa
    14761 cgcgtggtgc gtgaggcgac tatcgatgtt aacgcgctgg cggcgaagca gcctgtgacc
    14821 gaacaggaga ttgccagcta ctacgaacaa aacaaaaaca atttcatgac gccggaacaa
    14881 ttccgcgtga gttacatcaa gctggatgcc gcaacgatgc agcaaccggt tagcgatgcg
    14941 gatatccaga gctactacga tcagcatcag gatcaattca cccagccgca gcgtacccgc
    15001 tacagcatca tccagaccaa aactgaagat gaagcgaaag cggtacttga tgagctgaat
    15061 aaaggcggtg attttgctgc attagccaaa gaaaaatctg ccgatattat ctctgcccgt
    15121 aacggcggcg atatgggttg gttagaagat gccactatcc ccgacgaact gaaaaatgct
    15181 ggtctgaaag aaaaaggcca actgtctggt gtcatcaaat cttcggtcgg tttcctgatt
    15241 gtacgtctgg acgacattca gccagcgaaa gtgaaatcgt tagacgaagt acgtgacgac
    15301 attgcggcga aagtgaaaca cgaaaaagcc ctcgatgcgt actatgcgct gcagcagaaa
    15361 gtgagcgatg cggcaagcaa cgacaccgag tctctggccg gtgcagagca agctgccggc
    15421 gttaaagcca ctcagacggg ttggttcagc aaagataacc tgccggaaga gttgaacttc
    15481 aagccggttg ccgacgctat ctttaacggc ggtctggtag gtgaaaacgg cgcgccgggc
    15541 atcaactctg acatcatcac cgtagacggc gaccgtgcat tcgtgctgcg catcagcgag
    15601 cacaaaccgg aagcggtgaa accgttggca gatgttcagg aacaagttaa ggcactggtt
    15661 cagcacaaca aagctgaaca acaggcgaaa gtggatgctg agaaactgct ggttgatttg
    15721 aaagccggca aaggtgcgga agctatgcag gctgccggtc tgaaatttgg cgagccgaaa
    15781 accttaagcc gttccggtcg tgacccgatt agccaggcgg cgtttgcact gccactgcca
    15841 gcgaaagaca aaccgagcta cggtatggcg accgatatgc aaggtaatgt ggttctgctg
    15901 gcgctggatg aagtgaaaca aggttcaatg ccggaagatc agaaaaaagc gatggtgcag
    15961 ggtatcaccc agaacaacgc acaaatcgtc tttgaagctc tgatgagtaa cctgcgtaaa
    16021 gaggcgaaaa tcaaaattgg cgatgcgctg gaacagcaat aatcctgaag ccgcctcgca
    16081 aaaaaatgcg ttgcagctgt aacaagaaaa ggtcgctttc gcggcctttt ccatttctga
    16141 acattgccat ttgtttactg ttttcactgc cgttaaggtg attccactgt taacaaacaa
    16201 ggagaaaaca gtatgaaaca cggaattaaa gcactgctca ttaccctgtc cctggcctgt
    16261 gccggaatgt ctcatagcgc gctggcggca gcttctgtgg cgaaaccgac ggcggtagaa
    16321 accaaagcgg aagctcctgc agcacaaagt aaagcagcag taccggcgaa agccagtgac
    16381 gaagaaggca cccgggtcag cattaataat gccagcgcgg aagagctagc ccgcgcgatg
    16441 aatggcgttg gcctgaagaa agcgcaggcg attgtcagtt atcgcgaaga gtacggtccg
    16501 tttaaaacag tggaggatct aaagcaggtg ccggggatgg gcaattcgct ggtggaacgt
    16561 aatctggcgg tattaaccct gtaattaatt tgcatagtgg caattttttg ccagactgaa
    16621 gaggtcatac cagttatgac ctctgtactt ataacaacaa cgtaaggtta ttgcgctatg
    16681 caaacacaaa taaaagttcg tggatatcat ctcgacgttt accagcacgt caacaacgcc
    16741 cgctaccttg aatttctcga agaagcccgc tgggatgggt tggaaaatag cgacagtttt
    16801 cagtggatga cggcccataa catcgccttc gtcgtggtca atatcaatat taactatcgt
    16861 cgcccagcgg tattaagtga cctgttaact attaccagtc agttgcagca attaaacggt
    16921 aaaagcggca tcttaagcca ggtcattaca ctggagccgg aagggcaggt ggtagcggat
    16981 gcgcttatta cgtttgtttg tattgatctt aaaacgcaga aagcattagc tctggaaggg
    17041 gaattgcgcg aaaagctgga gcagatggtt aagtaaacgt tttgtggtgc cggatgctca
    17101 agccgcatcc ggcgacaccc ggaataatta cctcaacccg gttttctgct tcatcgctgc
    17161 catcaccgtc ggtttatcgg ccagataatg attcaaaccg ttggcgcgta aattacatgc
    17221 cgcacaatga ccgcaaccgt cgcctttaat gccgttatag caggtcaacg tttcgttacg
    17281 gactaaatcc agtttgccgt aataatctgc cagcgcccag gtttccgctt tatcaatcca
    17341 catcagcggc gtttcaaaac gaatatcttt cgccatgccc aaactgacgg catggtttag
    17401 tgctttcaca aactcatcgc ggcaatccgg gtagccggag aaatccgttt cacagacgcc
    17461 ggtaattacg gcttctgctt ttacctgata cgcatatatt gccgccagcg tcaggaacaa
    17521 aatattacgc cctgggacaa acgtattcgg gatgccatcg gcttcaggtt cataatcagg
    17581 caccggaatg ctgtcacgtg tcaggctgct gaccgccagc tcgttgagca gcgttacatc
    17641 aagaacctta tgcgcgcgtg ctcccagttt cagcgccagt tcgcgtgcca cgtcgatttc
    17701 tgcgcgatgt cgctgaccgt aatcgaacgt cacgcaatgg acttcatcat attgttgtaa
    17761 tgcctgcacc agacaggtgg tggaatcctg gcctccactg aacacaacga cagcacgttt
    17821 catagataat cctgcttgaa caataaaagc gttatggtaa cgcctgcgat taacccggac
    17881 cagctattca ttgcgacggc gcgggaagcc aggcttcggt aaattcaaac cagccgcgcg
    17941 gtgtcagtcg cacaccgttc acgccgggag gggcactaat gcgatagtga tagttgaaca
    18001 gcggcgtcag cgtcgcgtct gccattaact ggctaaaaac cgctttcagg gcattaaatc
    18061 ggttttcctc atcaggcatt acttgcaccg cgtccagcgt cgattgcaga tgtgcatatg
    18121 ctggagcgtc gaaaacatgt ggccatagtg gatcgcaacg tagccattgc tccagagtat
    18181 attccggtgc ttcgccaatt aatctgtcgc ccatcatgag gtctgcgtgt gccagtaggg
    18241 tcgtgtcgtc ccagtttttt gcgttatgaa aaataattgt gagttcacag ccttccgctg
    18301 ccagtgtcgc ctgtaggcgt tctgccatgg tatgaagttc tatcggtagg tgataaacca
    18361 gcgtcaaggt tttcggtagt ttgacttcat ccggtacttg ccagtgcgga atagtccagc
    18421 ctggcagtaa tgcatgactg gcggtgatca ggttttctcc gacttctaac gtttgtaata
    18481 aaccggattg atgaataatg gagatcactt ttcgcgcctg ccagagggag agtcggggac
    18541 ttttgcgcaa cgttaaatag caaaaaccta aactgatgcc actactgacc tggctgaccc
    18601 gttgcagctc ctccggtttg ccgatggtga tttgcacggg atgccgacaa ctggttccca
    18661 aatctttttc gaaaagcggc ggagttatcc agtactcaac cgctttaagc agcggatgac
    18721 gcaaatggta ataatcatgg ctctccaggc gcaccagctc cgctgtgaat tgtgttaagc
    18781 gaaaaggacc agtgccgatc agtgggaatt gcggatgcgc cagatggctg caatagctcg
    18841 ccagccggtg cgcaagccag taatcggggc gatgtaaaaa gaaggtcaga cactgcggat
    18901 gggtgacttc aatacgcttc acgctaataa ataattgatc cagtgctggc agttgtaaca
    18961 gcatcaataa tcgctggtgt aagtgtgagg cttttactgc atcgccgtta tgccagtgta
    19021 gggttgaacg aagataaaag tcccagcgta acccgtcagt agaggtttcc cagtgatgcg
    19081 ctaaatcgcc aatcgggcgc tgagtattat tatcgaagcg ggtcaggccg gaaaatatct
    19141 gcccggcgag atgctgctcg gcacggccgg gcaaaaagcc tggttgtagc ggttcgagcg
    19201 ggcgatagta gggaatacgc aacgtgggtg tatcgttttg ccattgtccg cccataaacg
    19261 gctgtaacag agtgcgtagc tcacctaggg ccagttgcgc cagctccagc acatcttgct
    19321 gctttccggt ttccagtgcc tgttccatca tcgcattgcg tagcgattcc ggcgtgacca
    19381 gaaagcgtaa ttgtccgcgc tttccgcgtc ctgactgcgc ctgccactcc agccatcccg
    19441 cctcctgtgc ctgacgcaac agcgtacgaa catggcgttc gctgcaaaaa cagcgttcgg
    19501 ccagttcgct gacggtgacg gtttgcggct ttccggctga aggttgccac agacgttgat
    19561 actggttaag acggttgagc aatcgcatat aaacccggaa caatattatt taactattca
    19621 ctattacttc cgtatatatc aggtgatact caatctccat taaccgtgtc acagagtgga
    19681 gaaagaaatg gctcgtctgg cagcatttga tatggatggc actttattga tgcctgacca
    19741 tcatttaggt gagaaaaccc tctctacttt ggcgcgactg cgtgaacgcg acattaccct
    19801 cacttttgcc acggggcgtc atgcgctgga gatgcagcat attctcgggg cgctatcgct
    19861 ggatgcatat ttgattaccg gcaacggaac gcgcgtgcat tctctggaag gtgaactttt
    19921 acatcgtgat gatttacctg cggatgtcgc ggagctggtg ctgtatcagc aatgggatac
    19981 ccgagccagc atgcatatct tcaatgacga cggttggttt accgggaaag agatccctgc
    20041 gttgttgcag gcatttgtct atagcggttt tcgttatcag ataatcgatg tcaaaaaaat
    20101 gccactcggc agcgtcacca agatctgctt ctgtggcgat cacgacgatc ttacacgctt
    20161 gcagatccag ctatacgaag cattaggcga gcgtgcacat ttgtgttttt ccgccacgga
    20221 ttgcctcgaa gtgctgccgg tgggctgcaa taaaggcgct gcattgacgg tgctgaccca
    20281 acatctaggt ttatcgttgc gcgattgcat ggcctttggt gatgcgatga acgatcgcga
    20341 aatgttaggc agcgtcggta gcgggtttat tatgggcaat gcgatgccgc aactgcgcgc
    20401 ggagctcccg catttaccgg tgattggaca ttgccgaaat taggctgtct ctcactattt
    20461 gacgcactga ctggactatc cacatctacc ttattccccc gaataatgag atcccttcca
    20521 gcaccgggca attgcccggt tttttttgcg ttgaatttgt cattttgtgc cgtggtgttt
    20581 aaaccgcaca gaataaattg tcgtgatttc acctttaaaa tagaattaaa agagaaaaaa
    20641 attctctgtg gaagggctat gttagataaa attgaccgta agctgctggc cttactgcag
    20701 caggattgca ccctctcttt gcaggcactg gctgaagccg ttaatctgac aaccacccct
    20761 tgctggaagc gcctgaaacg gctggaggac gacggtatcc ttatcggcaa agtcgccctg
    20821 ctggatccgg aaaaaatagg cctcggcctg accgcctttg tgctgataaa aacgcaacat
    20881 cacagtagcg aatggtattg ccgctttgtc acggtggtta ccgaaatgcc agaagtgctg
    20941 gggttctggc gcatggctgg tgaatacgat tatctgatgc gcgtccaggt tgccgacatg
    21001 aaacgctacg acgagtttta taagcgtctg gtaaacagcg tgccggggct gtcggacgtc
    21061 acttccagct tcgcgatgga acagattaaa tacaccactt ctttacccat cgaataaata
    21121 tccagaatca ggtcaggaca caacgcgtgc gattatttgc tcaattaagc tggtatttcc
    21181 gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct tgtcattatc gcgatgctgc
    21241 aactggttcc gccaaaagtg gttggtattg ttgtcgatgg cgtgacagaa caacacttta
    21301 ctaccgggca gatcctgatg tggatcgcca ccatggtgct gattgccgtt gtggtttatt
    21361 tcctgcgtta cgtctggcgg gtattgctgt ttggtgcgtc ttatcaactg gctgttgaac
    21421 tgcgtgaaga ttattaccgt cagctaagcc ggcagcatcc tgagttttac ctgcgtcatc
    21481 gcacgggtga cctcatggct cgtgcgacca atgacgtcga tcgcgtcgtg tttgcagccg
    21541 gagaaggggt gctgacgctg gtggattcac tggtgatggg ctgcgctgtg ttgattatga
    21601 tgtctacgca aattagctgg cagttgacct tatttgccct gttgccgatg ccagtgatgg
    21661 cgatcatgat caagcgcaac ggcgatgcct tgcatgaacg ctttaagctg gcacaggcgg
    21721 cgttttctag tcttaatgac cgcacccagg aaagcctcac cagtatccgc atgatcaaag
    21781 cctttggtct ggaagatcgc cagtcggcgt tatttgccgc ggatgccgaa gataccggca
    21841 aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga cccgaccatc tatatcgcga
    21901 ttggtatggc gaatttgctg gcgattggcg gcggtagttg gatggtggtg cagggcagtt
    21961 taacgctggg ccagctcacc agttttatga tgtatttagg tctgatgatt tggccaatgc
    22021 tggcgctggc atggatgttt aacattgtgg aacgtggtag tgctgcatac agccgtattc
    22081 gcgcgatgct ggcggaagcg ccggtggtga acgatggtag cgaacccgtg ccggaagggc
    22141 gtggcgaact ggatgtaaat attcaccagt tcacgtatcc gcagactgac catcctgcgc
    22201 tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct gggtatctgc gggccgactg
    22261 gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca tttcgacgtc agcgaggggg
    22321 atattcgctt tcatgatatt cctctgacga agttacaact cgatagctgg cgtagccgcc
    22381 tggcggtagt tagccagaca ccgttccttt tttccgacac cgtagcgaac aacatcgcgt
    22441 tgggttgccc gaatgccacc cagcaagaga ttgagcatgt cgcgcggtta gccagcgtac
    22501 atgacgatat tttgcgtcta ccgcaaggtt acgatacaga agtgggcgag cgcggcgtga
    22561 tgctttccgg tgggcaaaaa cagcgtatct ccattgctcg agcgttatta gtcaacgcgg
    22621 aaatcctcat ccttgatgat gcgctttcgg cggtggacgg acgcacagag caccagatcc
    22681 tgcataacct gcgtcagtgg ggacagggaa gaacggtaat catcagtgcc catcgccttt
    22741 cggcactgac ggaagccagt gaaaatattg tgatgcagca cggacatatc gcccagcgtg
    22801 gcaatcatga tgagctggcg caacaaagcg gctggtatcg cgatatgtat cgctatcaac
    22861 aactggaggc ggcgctcgac gacgctccgg aaattcgcga ggaggccgtc gatgcgtagt
    22921 tttagccaac tgtggccgac tctcaagcgt ctgttagcgt acggttcgcc gtggcgtaaa
    22981 ccgctgggga ttgcggtcct gatgatgtgg gttgcggcag cggcagaagt cagtgggccg
    23041 ctgcttatca gctattttat cgacaatatg gtagcgaaaa ataacctgcc gttgaaaatg
    23101 gttgcagggc tggctgcggc gtatgttggg ctacaactgt ttgccgccgg gctacattac
    23161 gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag tgcaacagtt gcgtaccgac
    23221 gtgatggatg ccgcattgcg ccagccatta agcgagtttg atacccaacc cgtcgggcag
    23281 gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg atctctacgt taccgtagtg
    23341 gcaactgtcc tgcgtagcgc cgcactggtg ggcgcgatgc tggtggcgat gttcagcctc
    23401 gactggcgaa tggcgctggt ggcgataatg attttcccgg tggtgctggt ggtaatggtg
    23461 atataccagc gttacagcac gccgattgtc cgtcgtgtgc gcgcctatct ggcggatatc
    23521 aacgacggct ttaacgaaat catcaatggc atgagcgtta tccagcagtt ccgtcagcag
    23581 gcgcgatttg gcgaacgtat gggggaggcc agtcgttcac actatatggc caggatgcaa
    23641 accctgcgcc tcgacggttt tctgctgcgt ccgctgctga gtctgttttc atcgctcatt
    23701 ctttgtggct tgttgatgct gtttggcttc tccgctagcg gcaccattga agtaggcgtg
    23761 ctgtatgcgt ttatcagcta tcttgggcga cttaacgaac ccttaatcga actgaccacg
    23821 caacaggcga tgctgcaaca ggctgttgtt gctggtgagc gcgtgtttga actgatggac
    23881 ggaccgcgcc agcaatatgg caatgatgat cgcccgttac agagtggcac catcgaagtc
    23941 gataacgtgt catttgctta tcgcgatgac aatctggtgc taaagaacat taatctctct
    24001 gtgccttcgc gtaactttgt ggcactggtc gggcataccg gcagtggcaa aagcaccctc
    24061 gccagtttat tgatgggcta ttacccgcta acggaaggtg agattcgcct tgatggtcgt
    24121 ccattaagtt cgctaagtca cagcgcgctg cgccagggcg tggcaatggt gcagcaagat
    24181 ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc tggggcgtga tatctccgaa
    24241 gaacgcgtct ggcaggcgct ggaaaccgtg caactggcgg agctggcgcg cagcatgagt
    24301 gatggtattt atacgccgct gggcgagcag gggaataatc tctccgtcgg gcaaaagcaa
    24361 ctgctggcac tggcacgcgt gttggtggag acgccgcaaa tcctgatcct tgatgaggca
    24421 accgccagca ttgactccgg tactgaacag gcgattcaac atgctctggc ggctgtgcgt
    24481 gaacatacca cgctggtggt gattgctcac cgcttatcga ccattgttga tgccgacacc
    24541 attctggtgc ttcatcgtgg gcaagccgtg gagcagggca ctcaccagca actgctggcg
    24601 gcccagggac gctactggca gatgtatcaa ctgcaacttg cgggcgaaga gctggcagcc
    24661 agcgtgcgtg aagaagaatc attgagcgcc tgaatagcgc aatatttcat cgttggtgca
    24721 aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat tttcaatctt cttaacttcc
    24781 tgctctcttt ctcgtttttc atttctggca caccgcttgc aataccttct tcgtgtagca
    24841 gaaccattac cgaattctga ctggagggga tctatgaagc tggtgaccgt gataatcaaa
    24901 ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca ttggtattca gggcctgacc
    24961 gtcaccgaag tgaaaggttt cgggcgtcag aaagggcatg ccgagctgta ccggggggcg
    25021 gaatacagcg tcaacttcct gccaaaagta aaaattgatg tggcaattgc tgatgaccaa
    25081 ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca ccggaaaaat tggcgacggc
    25141 aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc gtaccggcga agccgacgaa
    25201 gcggcgctgt aatctctggc acacagcaac aggaacgaaa aatgaagata gcgacgataa
    25261 aaactgggct tgcttcactg gcgatgctgc cgggactggt aatggctgca cctgcggtgg
    25321 ccgataaagc cgacaatgcg tttatgatga tttgtactgc gctggtgctg tttatgacta
    25381 ttccggggat tgccctgttt tacggtgggt tgattcgcgg caaaaacgtg ctgtcgatgc
    25441 tgatgcaggt gacggtgacg tttgcactgg tctgtattct ctgggtggtt tacggttact
    25501 cgctggcgtt tggtgagggt aacaacttct tcggcaacgt taactggttg atgctgaaaa
    25561 acatcgaact gacggcggtg atgggcagca tttatcagta tatccacgta gcgtttcagg
    25621 gatcgtttgc ctgcattacc gtaggcttga tagttggggc gctggcggaa cgaatccgct
    25681 tctcagctgt gttgattttc gtggtggtat ggctgacgct ctcttacatt ccgattgcgc
    25741 atatggtgtg gggcggcggt ttgctggctt ctcacggtgc actggatttc gcgggtggca
    25801 cagtggtgca cattaacgcc gcaatcgccg gtctggtggg cgcgtatctg ataggcaaac
    25861 gcgtgggctt cggtaaagag gcgtttaaac cgcacaacct gccgatggtc ttcaccggga
    25921 ctgccattct ctatatcggt tggtttggct ttaacgccgg gtcagcgggc acggcgaatg
    25981 aaatcgcggc actggcattt gtgaatactg tggtcgcaac ggcggcggca attcttggct
    26041 ggatcttcgg tgaatgggcg ctgcgtggta agccttcact gctgggggcg tgttctggcg
    26101 cgattgccgg tctggtcggc gtgacgccag cgtgcggcta cattggggtt ggcggcgcgt
    26161 tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg cgttaccatg ctcaaacgct
    26221 tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca cggcgtttgt ggcattgtcg
    26281 gctgtatcat gaccgggatt tttgccgcca gctcgctggg cggcgtgggc ttcgctgaag
    26341 gtgtgacgat gggtcatcag ttgctggtac agctggaaag catcgccatt acgatcgtct
    26401 ggtccggtgt ggtggcattt attggctaca aattggcgga tctgacggtt ggtctgcgtg
    26461 taccggaaga gcaggagcta gaagggctgg atgtcaacag tcacggcgag aatgcctata
    26521 acgcgtaaca agcactgcaa aaaacagccg gacggttttc acctccggct atttttttaa
    26581 ttgtgattac gcatcacccc ttcctgaacg gtcgaggcaa ccagtacgcc gtcctgggta
    26641 taaaactcac cgcgcacaaa gccacgtgcg ctggacgccg aggtgctctc cacgctatac
    26701 agcagccatt cattcaaatt aaacgggcga tggaaccaca tggaatggtc aatggtggca
    26761 atctgaatcc ccggttcgag aaaaccgatg ccgtgcggct gtagagctac cggcaggaag
    26821 ttaagatcag aagcgtaacc gagcagatac tgatgaacgc gcaggtcatc cggcacgcta
    26881 ccatttgcgc ggatccacac ctgacgatgt ggttctgcga cgtgaccttt cagtgggtta
    26941 tgaaactcca ccggacggac ttccagcgga cgatcgcaga tgaatttatc tttaagcact
    27001 ggcggcagca ggtgcgccag cgattgggcg atttgcgttt ccgaaggcag gccatcaggc
    27061 gctggcgcgg acggcattgt tttttgatgt tcgaaacccg cttctggtgc ctggaaagag
    27121 gccgtcatat aaaaaatcgg tttgccgttt tgaatagcag caacccggcg ggcgctgaag
    27181 ctgttaccat cacgcagcgt ttcgacatca taaataatcg gtttcttact atcgcctggg
    27241 cgaagaaagt agctgtgaaa cgaatgtacc agccgctctt ccgggacggt ctcttttgca
    27301 gcatacaagg cctgacccac gacctggccg ccaaacacct ggcgtaaacc taaatcttca
    27361 ctctggccgc gaaagagtcc tgcctcaatt ttttccagat ttaacaatgc cagtaaattt
    27421 tttagcgcct gactcatata actctccagt aacaaagctg ccgcagcaag ccaaagtgag
    27481 ttgagtataa cgcaaatttg ccactggtcc gatgggtgca ataatctgaa ttacgggcta
    27541 attacaggca gaaatgcgtg atgtgtgcca cacttattga cgttactatt ttgttaacca
    27601 ctcttccggc gaggaaagtt agcccgctgg tgcattgata ataaggagaa atgaatgaaa
    27661 ctcgtgcaca tggccagtgg tttagcggtt gcgattgcgt tggcggcttg cgcagataaa
    27721 agcgcggata ttcagacgcc agccccggct gcaaatacgt ctatttcagc aacacaacaa
    27781 cctgctatcc agcaaccgaa tgtctccggt accgtctgga tccggcagaa agtcgcactg
    27841 ccgcctgatg ctgtgctgac cgtgacactt tctgacgcgt cgttagccga tgcaccgtca
    27901 aaagtgctgg cgcagaaagc ggtgcgtacc gaaggtaaac agtcaccatt cagctttgtt
    27961 ctgccattta acccggcaga tgttcagccg aacgcgcgta ttctgttgag tgcggcgatt
    28021 accgtgaatg acaaactggt atttattacc gataccgttc agccggtgat caacaagggc
    28081 ggaactaaag ccgacctgac attggtgccg gtgcagcaaa ccgccgtgcc ggttcaggcc
    28141 agcggtggtg caacgactac cgtaccttcg acttcaccaa ctcaggtgaa tccgtcttcg
    28201 gcagttccag ctcctacgca atattaagcg acgttaaccc tc
//
LOCUS       DAJTOR010000035        28152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTOR010000035 DAJTOR010000000
VERSION     DAJTOR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.035"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(383..1483)
                     /gene="chaA"
                     /locus_tag="OO249_001371"
     CDS             complement(383..1483)
                     /gene="chaA"
                     /locus_tag="OO249_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5635725.1"
                     /translation="MSNAQEAVKTRHKEISLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1753..1983
                     /gene="chaB"
                     /locus_tag="OO249_001372"
     CDS             1753..1983
                     /gene="chaB"
                     /locus_tag="OO249_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5635726.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2141..2836
                     /gene="chaC"
                     /locus_tag="OO249_001373"
     CDS             2141..2836
                     /gene="chaC"
                     /locus_tag="OO249_001373"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5635727.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2880..3233)
                     /gene="ychN"
                     /locus_tag="OO249_001374"
     CDS             complement(2880..3233)
                     /gene="ychN"
                     /locus_tag="OO249_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5635728.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3417..4811
                     /gene="ychO"
                     /locus_tag="OO249_001375"
     CDS             3417..4811
                     /gene="ychO"
                     /locus_tag="OO249_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5635729.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYFTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLLANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEYNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4812..5462)
                     /gene="narL"
                     /locus_tag="OO249_001376"
     CDS             complement(4812..5462)
                     /gene="narL"
                     /locus_tag="OO249_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5635730.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5455..7251)
                     /gene="narX"
                     /locus_tag="OO249_001377"
     CDS             complement(5455..7251)
                     /gene="narX"
                     /locus_tag="OO249_001377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5635731.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRVRLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7590..8981
                     /gene="narK"
                     /locus_tag="OO249_001378"
     CDS             7590..8981
                     /gene="narK"
                     /locus_tag="OO249_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5635732.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9497..13219
                     /locus_tag="OO249_001379"
     CDS             9497..13219
                     /locus_tag="OO249_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5635733.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            13237..14775
                     /gene="narH"
                     /locus_tag="OO249_001380"
     CDS             13237..14775
                     /gene="narH"
                     /locus_tag="OO249_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5635734.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            14772..15482
                     /gene="narJ"
                     /locus_tag="OO249_001381"
     CDS             14772..15482
                     /gene="narJ"
                     /locus_tag="OO249_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5635735.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEVIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            15482..16159
                     /gene="narI"
                     /locus_tag="OO249_001382"
     CDS             15482..16159
                     /gene="narI"
                     /locus_tag="OO249_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5635736.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFAGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(16342..16473)
                     /locus_tag="OO249_001383"
     ncRNA           complement(16342..16473)
                     /ncRNA_class="other"
                     /locus_tag="OO249_001383"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(16521..16605)
                     /locus_tag="OO249_001384"
     tRNA            complement(16521..16605)
                     /locus_tag="OO249_001384"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16569..16571),aa:Tyr,seq:gta)
     gene            complement(16640..16724)
                     /locus_tag="OO249_001385"
     tRNA            complement(16640..16724)
                     /locus_tag="OO249_001385"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16688..16690),aa:Tyr,seq:gta)
     gene            complement(16884..17726)
                     /gene="purU"
                     /locus_tag="OO249_001386"
     CDS             complement(16884..17726)
                     /gene="purU"
                     /locus_tag="OO249_001386"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5635737.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(17776..18234)
                     /locus_tag="OO249_001387"
     CDS             complement(17776..18234)
                     /locus_tag="OO249_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5635738.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            18347..19252
                     /gene="rssA"
                     /locus_tag="OO249_001388"
     CDS             18347..19252
                     /gene="rssA"
                     /locus_tag="OO249_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5635739.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQCREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRVLGADIVIAVDLQHDAHLIQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            19344..20357
                     /gene="rssB"
                     /locus_tag="OO249_001389"
     CDS             19344..20357
                     /gene="rssB"
                     /locus_tag="OO249_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5635740.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            20559..21467
                     /gene="galU"
                     /locus_tag="OO249_001390"
     CDS             20559..21467
                     /gene="galU"
                     /locus_tag="OO249_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5635741.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(21611..22024)
                     /gene="hns"
                     /locus_tag="OO249_001391"
     CDS             complement(21611..22024)
                     /gene="hns"
                     /locus_tag="OO249_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5635742.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            22629..23246
                     /gene="tdk"
                     /locus_tag="OO249_001392"
     CDS             22629..23246
                     /gene="tdk"
                     /locus_tag="OO249_001392"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5635743.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(23528..24424)
                     /locus_tag="OO249_001393"
                     /pseudo
     CDS             complement(23528..24424)
                     /locus_tag="OO249_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(24548..27223)
                     /gene="adhE"
                     /locus_tag="OO249_001394"
                     /gene_synonym="adhC"
     CDS             complement(24548..27223)
                     /gene="adhE"
                     /locus_tag="OO249_001394"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5635744.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGILSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            27701..>28152
                     /gene="ychE"
                     /locus_tag="OO249_001395"
     CDS             27701..>28152
                     /gene="ychE"
                     /locus_tag="OO249_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5635745.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYL"
BASE COUNT         6924 a         6779 c         7338 g         7111 t
ORIGIN      
        1 aacattttgc ggctattctt gaagtgtctg gtttcaagat tagcccccgt tttgttgtca
       61 ggttttacct ctcaacgtgc gggggttttc tctttccagc aaccaatgcc accagggata
      121 aagcccccgc aacattgcgc ctcaccggat aattccggct tggtgtggat actacgtctc
      181 aattcatctt cacttcatcc ctgaaatgtt tgcaataaag agtacattcc ggcttttcaa
      241 cagctgttgc agtggtttcg tgcaggttgt ggtacaggct cgcaattctg attaacgagc
      301 tgtccaggta gtatgaagaa atacaataag caggaattta tcttatctcc cccgatttcc
      361 gtcatcggag gagatataac tgtcaggcaa atatcgtcat caaataggcg gcaaacagtg
      421 ccagatgcgc tgcgccattg agcacgttag tacgtccggt ggagaaggag atatggcaca
      481 gcactaaaga ggccaccatc accaccattt ctggcgcacc aagtgcaaac tgtaattcgt
      541 tacccgtcat aaaggcaatt agcgtgacga caggtacggt aagtgaaatg gttgctaaca
      601 ctgaaccaaa gaacagattc atcgcgcgct ggacctggtt gttcaacact gcttttaacg
      661 cacctaaacc ttccggcgac agaatcaaca gagccaccag gaagccagta aaggcgacag
      721 gggcattcat gctgtcgagc aatgtctcca gcgggctggc attcattttg gtgactgcaa
      781 taacggcaat cagatggata atcaaccaga tagcatgcca cacgctgcta tgggcagacg
      841 gtttaccgtg atgcgggtcg tcgtcatcac tgtcatcttc gtgctcgtag acaaacaaac
      901 tttgatgcgt tttggtctgg atcagcaaaa atacgccata cattgccgca gaaattaatg
      961 ctaccagtaa cgcctgaccg gttgaaaaat tcgccgcagg cagagccatt ggaaatacca
     1021 gtacgattat cgccaggggg aacagcgcaa ttaaatactg cttgatacca aacagattca
     1081 tatattgggt ggcaaactta cgaccgccca acaataatga aaagccaacc agcccaccgg
     1141 taacaatcat aatgattgaa tagagcgtat cacgcattag cgttggcgcg gcgtcgccgg
     1201 ttgccattaa agctgaaatc aaactgactt caagaataac cactgaaagg ctaagaataa
     1261 gcgaaccgta aggttctccc aggcgatggg ctaatacgtc cgcatgacgg acaacactaa
     1321 aggcgctact taaaatacca ataagcgcaa gaagattgat ggcaatgacc actggtagtg
     1381 tctggctgct tccccacagg aacagcacta ccagcgccag aaccgggaaa ataagcgaaa
     1441 tctccttgtg gcgggttttt accgcctctt gagcatttga cattatggtt atccctttgc
     1501 agatgaattt atcgaaaatg taaaaaatag gtaggaaaaa taacagaaag tgtctggata
     1561 tcggtaacat tttacgaatt tttaccccac tgtcgatttt ttactcattg ggacataaaa
     1621 ataagtatta cgtttagaca atgtttgttt tagcgtttct taagaacagt ctgacatgaa
     1681 aattcttatg ttttggcaag agtagatatt gttgaccaca cttaatgttc aactttgtaa
     1741 aaggagtcaa cgatgccgta taaaacgaaa agcgatctgc cggaaagcgt aaagcacgtt
     1801 ctaccgtctc atgcccagga tatctataaa gaagcgttca acagcgcatg ggatcaatat
     1861 aaagataaag acgatcggcg tgatgacgcc agtcgcgaag aaaccgcgca taaagtggcc
     1921 tgggctgctg tgaagcatga atatgccaaa ggggatgatg ataaatggca taaaaaatcg
     1981 taaaaccggt agcttagtta aagctattcg tgcggtgttg ccttgcaagt ggtccgtgga
     2041 ttgcatattg tcccgttagt ggtttcaaaa tgagcagtaa aaatgtccgg aagacaccaa
     2101 aaagttgtcg cagggaagta tgcagtggcg gaggtgtaag gtgataacgc gtgatttctt
     2161 gatgaatgcc gattgtaaaa cggcatttgg tgccattgaa gaatcactct tatggtcagc
     2221 agagcaacga gcggcttcgc tggcggcgac gctggcttgt cgacctgatg agggaccggt
     2281 gtggatcttc ggttatggat cgttgatgtg gaatccggca ctggagttta ccgagtcgtg
     2341 caccggtaca ctggttggat ggcatcgcgc gttttgcctg cgcctgaccg ccgggcgcgg
     2401 gactgcgcac cagccgggac ggatgcttgc actgaaagag ggcggacgca ctacaggcgt
     2461 cgcctatcga ctgccagaag agacgctgga gcaggaacta accctgttgt ggaagcgcga
     2521 gatgattacc ggctgttatc tgccaacctg gtgtcagctt gatcttgatg atggatgcac
     2581 agtaaacgcc attgtgttta ttatggaccc gcgacatcca gaatatgaat ctgacactcg
     2641 cgctcaggtc atcgcgccgt tgattgcggc ggcgagcggt ccgctgggaa ccaacgcaca
     2701 atacctgttt tcactggaac aggagcttat caaactggga atgcaggacg atgggctgaa
     2761 tgatttgctg gtatcggtaa aaaaactgct ggcggagaat tatccggatg gtgtgttacg
     2821 gccgggattc gcctgagtaa acttcccgca tagtggggcg tcagacgccc cctcaaacat
     2881 taaaatgtga gcactttatc ggctgacagc gtccattgcg ccagttccac aagagtaccg
     2941 atttccaccc catcaatcag aggaagtgta ctaatcccgc gcccgtcggt acaggttttg
     3001 cacaatttca ccggtacatt ctgagcggta aggatctcca gcatttgctg aatgttgtag
     3061 ccttcccctg gtttttgccc gcgcaacccg gctgtgaccg catcagacat gaggaacaga
     3121 cgcagatcca gattgctctc ctgctctcgt aacgcaatgg ccagccgcaa gctgttaaac
     3181 aaggattcgc tcccgtaagg tgcgccattg gcaacgatca cgattttttg cattatttac
     3241 tcctgtattc agggaattag acactcatct tctatcttac tgcttctgca gcgtctgacc
     3301 aatcggtcac atttttaagg attttcctga aagcgcgaga aaatacgaca aaagttgcca
     3361 gtaatcgtta ttctttaagg ctatggtttt cattattacc ggaagttacc gacgttttga
     3421 gccgtttcgt tcctcgcatt atcccgtttt atttactctt gcttgtagca ggcggtacag
     3481 ctaacgcaca atctaccttc gagcaaaaag cggcaaatcc ctttgataat aacaatgatg
     3541 gtctgccgga tttaggcatg gctcccgaaa atcatgatgg ggaaaaacac tttgctgaaa
     3601 ttgtgaaaga tttcggcgaa accagtatga atgataacgg gctggatact ggcgagcagg
     3661 caaaagcttt cgcattggga aaagtccgcg acgcgcttag tcaacaggtt aatcagcacg
     3721 tagagtcctg gctatcaccg tggggaaatg ccagtgttga cgtcaaagtg gataacgaag
     3781 gacatttcac cggcagtcgt ggaagctggt ttgtgccgtt acaagataat gatcgttatt
     3841 tcacctggag ccagcttggt cttactcagc aggataatgg gttggtgagc aatgtgggcg
     3901 ttgggcaacg ctgggcgcgc ggcaactggc tggtgggtta taacactttt tatgacaact
     3961 tgctggacga aaatcttcag cgagcgggct ttggtgccga agcgtggggc gaatatttgc
     4021 gattattggc aaacttttat cagccgtttg ctgcatggca tgaacagaca gccacgcagg
     4081 aacaacggat ggcgcgcggg tacgacctga cagctcggat gcgcatgccg ttctatcaac
     4141 acctcaatac cagtgtcagc ctagaacagt attttggtga tcgtgttgat ttgtttaact
     4201 ctggtacggg ttatcacaat cccgtcgcgt tgagtctggg attaaattac acccctgtgc
     4261 cattagtcac tgtgacggcc cagcataaac agggtgaaag tggcgagaat caaaataacc
     4321 tcgggctgaa tcttaattac cgctttggtg taccgctcaa aaaacaactt tctgcgggcg
     4381 aggttgccga aagtcagtcg ttacgtggta gtcgctatga caatccgcag cgaaataatc
     4441 taccgactct tgagtaccga cagcgaaaaa cgttaacggt gtttctggcg acaccgccgt
     4501 gggatctaaa acctggcgaa acagtgccgc tgaaattaca aatccgcagt cgttacggta
     4561 ttcggcaact gatttggcag ggcgatacgc agatattaag tttgacgccg ggcgcacaag
     4621 ccaacagcgc ggagggctgg acgctgatca tgcctgactg gcagaacggg gaaggggcaa
     4681 gcaatcactg gcgattgtcg gtggtggtgg aatataacca ggggcagcgt gtctcctcca
     4741 atgagatcac gctaacgctt gtcgaaccgt tcgatgcatt gtcaaacgac gaactgcgct
     4801 gggaaccgta atcagaaaat gcgctcctga tgcacccata ccgctgcttc cacgcgagac
     4861 ttgagcttca ttttcttcag catgtgcttg acgtgcactt ttactgtgct ttcggtgata
     4921 tccaggcggc gggcaatcat cttgttcggc aaaccctggg caatcagctt gagaatatcg
     4981 cgctcgcgtg gggttaactg gttaacatcg cgctcagtag tggcacggtt agcgcgcaag
     5041 ctggcggcca gaacaggcgt taatgcttcg cttaatacca tttcgccagc agctgcctga
     5101 tgcaatgctt tcagcagatc ttccggttcc atatctttta acagataacc atccgcgccg
     5161 cgtttcagtg cggtgaccac atcttcttca tggttagaga cgctgaatac cacaatgcgc
     5221 cctgagaggg acttttcgcg cagtttatcc agcgtttcca gaccgttcat gccgggcata
     5281 ttgagatcta acaggatcag atcgggatca agagactccg ccagttcaat accctgttcg
     5341 ccattactcg cttcgccaac cacggtgata tctggtgcca tactgataag ctgttttacg
     5401 ccagttcgca gcatcgggtg atcgtcgatc agcaggatag tagccggttc ctgattactc
     5461 atgggtatct ccttggacgt ctgtgaaagt tttttcggga ataaaggtga ccaccacttc
     5521 ggtgccacct gattcacgac ggcggacgcg gcaatcgcct cgtaaacttt gcgcacggtc
     5581 gcgcattatt atcatgccgt agtgattgct gcggatggca ttttcaggca cgccgcagcc
     5641 gttatcttgg acggtcagtt tgacctgatt atcgttttgc gccaccgtca ccacgacctc
     5701 actcgcttgc gaatgtttga gggcgttact taatgcctca cgggcaattt gcaacaagtg
     5761 gattgcctga tgtgaaggca ccagacgagg cggcaattga taatccagct tcaccgggaa
     5821 gccaaatttg gcgctgtact cttcgcaact cgcctccagc gccggacgta atccaggctc
     5881 ggtgagctgc aagcggaatg tggtgagcaa ttcacgcaac tgcgcccagg atgcattcag
     5941 ttcgttacgg atctgactta acagttcgcg gctgcttgct ggcagcgcat cgccctgcat
     6001 ctgtaaacaa ctcacctgca tcttcatgca agagagagat tgggcaatag aatcatgcag
     6061 ttcgcgcgca atggtggcac gctcttccat cacgatcaac tgttgctgac gttcctgatg
     6121 gcgatccagc gccagcgtgg cggtgagttg ttcaaccagg gtatccacca gttgttgttg
     6181 atcatggcta agatgacgcc cctgcggcag ggtcgccagc aaaataccgt actgcgtatg
     6241 agagtcagcc agccgccact tcagggttgt gccgcgatca ccaacgggta atacgccgcg
     6301 cgggcagagc tggcagcctt tatcatcaca agtcatatct ggctggcagg taaactcctg
     6361 atgattctct tcatcatcag tgtcatacac ccgcagttcg atatcacgta gcagggttaa
     6421 attctgtaag ccgttgagta caggtgacag gcgttcacac agcggggcgc gggaatgcaa
     6481 acggcggtta gcctgccata aaaaagagag gatctgattt ttatgctcca gcccggcggt
     6541 tttctcctga acccgctgct caagtacggc ataactttcg gccagttctg cagacatatt
     6601 gttcaatgca gttccaagca tcgccatttc gttgcgcccg ctgatgttcg cgcgttgggt
     6661 aaaatcgcga tgactgacgg cactcgccat tgccagcagt tgccgccacg gttgtagcag
     6721 tcgcacccgc aaccagataa tagtgaacac cagtaaaagt gccataaata ccgccattac
     6781 ccgatggacc agtaccactg tctcaatgcg catttccgtg gtgcggtcaa aaccagatac
     6841 cagctgatca agcccggcaa caaactggct gacatccgct gacaccgttt cgcggttttg
     6901 tgcacgcatc agtgcaggga tcagttcatt acgccagtaa tcttgtaaac cctgtaattg
     6961 cgccagttgt tcgtctcgtt ctgctgctcg agttaactcg gcgctaaatg ccgtttgttc
     7021 catctcttta attaagggct tgtctttctc acttaatggc actgccgcca acagacggta
     7081 actttgcatg cgcagcgatc ccgctttgtt gatcgcatgg gcgctgccct gaacgccttg
     7141 caccagccag ccagaaaccg ccatccctgc cagtccaata gcagtagaaa gcaacacaat
     7201 aagcgcaacc tgattaacca gggtgagcgg agagagacaa cgtttaagca tgtaaacctc
     7261 ttccttcagg ctttaaatga gcaataacct taatgaatgt gacgatacat tctggaatgg
     7321 cagtattctc ggctattggc tgaagtatac ccatacccgg aaagagttac tccttatttg
     7381 ccgtgtggtt agtcgcttta catcggtaag ggtagggatt ttacagcacc gtgaaaaatc
     7441 tcataatttt tatgaaatca ctgtactcac tatgggtaat gataaatatc aatgatagat
     7501 aaagttatct tatcgtttga tttacatcaa attgccttta gctacagaca ctaaggtggc
     7561 agacatcgaa acgagtatca gaggtgtcta tgagtcactc atccgccccc gaaagggcta
     7621 ctggagctgt cattacagat tggcgaccgg aagatcctgc gttctggcaa caacgcggtc
     7681 aacgtattgc cagccgtaac ctgtggattt ccgttccctg tctgctgctg gcgttttgcg
     7741 tatggatgtt gttcagcgct gttgcggtga acttaccgaa agtcggtttt aattttacga
     7801 ccgatcagct atttatgttg actgcgctgc cttcggtttc tggcgcgtta ttacgtgttc
     7861 catactcctt tatggttcct atcttcggtg gtcgtcgctg gacggcgttc agcaccggta
     7921 ttctgattat tccttgcgtc tggctgggtt ttgccgtgca ggatacctcc acgccttata
     7981 gcgtcttcat catcatctct ctgctatgcg gctttgctgg cgcgaacttc gcatccagta
     8041 tggcaaacat cagcttcttc tttccgaaac agaagcaggg tggcgcgctg ggtctgaatg
     8101 gtggtctggg aaacatgggc gtcagcgtca tgcagttggt tgctccgctg gtggtatcac
     8161 tgtcgatttt cgcagtattt ggtagccagg gcgtcaaaca gccggatggg actgagctgt
     8221 atctggcgaa tgcgtcctgg atatgggtgc cgttccttgc catcttcacc attgcggcgt
     8281 ggtttggcat gaacgatctt gctacctcga aagcctccat caaggagcag ttgccggtac
     8341 tcaaacgggg tcatctgtgg attatgagcc tgctgtatct ggcaaccttc ggctccttca
     8401 tcggcttctc cgcgggcttt gcaatgctgt caaaaacgca gttcccggat gttcagattc
     8461 tgcaatacgc tttcttcggg ccgtttattg gtgcgctggc gcgttctgca ggtggtgcat
     8521 tatctgaccg tctgggcgga actcgtgtca cgctggtgaa ctttattctg atggcgattt
     8581 tcagcggcct gctgttcctg accttaccga ctgacgggca gggcggaagc ttcatggcgt
     8641 tcttcgcggt cttcctggcg ctgttcctga cagctgggct gggtagtggt tccactttcc
     8701 agatgatttc agtgatcttc cgtaaactga caatggatcg cgtgaaagca gaagggggtt
     8761 ctgacgaacg tgcgatgcgt gaagcggcaa ccgacacggc ggcggcgctg ggtttcatct
     8821 ctgcgattgg cgcgattggt ggcttcttta tcccgaaagc gtttggtagc tcgctggcat
     8881 taacgggttc gccagtcggc gcaatgaagg tatttttgat tttctatatc gcctgcgtag
     8941 tgattacctg ggcggtatat ggtcggcatt ctaaaaaata aaccgttact cgtcatactc
     9001 cgggttacat gtgctgcggc tgcgttcatt caccccagtc acttacttta gtaagctcct
     9061 gggattcatt cacttgccgc cttcctgtaa accgaattat atagagtaaa atatttgatt
     9121 atcctttgcg cggcatgatg tcgcgctttt tttaagcgtt atttagttac aacatactaa
     9181 tgttatatgg tttatttcgc cggatttcat taagagccat taatatgtta cccatgggga
     9241 atactcctta atacccatct gcataaaaat cttaatagtt taaataacta caggtataaa
     9301 acgtcttaat ttacagtctg ttatgtggtg gctgttgatt atcctaaagg ggtatcttag
     9361 gaatttactt tatttttcat ccccatcact cttgatcgtt atcaattccc acgctgtttc
     9421 agagcgttac cttgccctta aacattagca atgtcgattt atcagagggc cgacaggctc
     9481 ccacaggaga aaaccgatga gtaaattcct ggaccggttt cgctacttca aacagaaggg
     9541 tgaaaccttt gccgatgggc atggccagct tctcaatacc aaccgtgact gggaggatgg
     9601 atatcgccag cgttggcagc atgacaaaat cgtccgctct acccacgggg taaactgcac
     9661 cggctcctgc agctggaaaa tctacgtcaa aaacggtctg gtcacctggg aaacccagca
     9721 gactgactat ccgcgtaccc gtccggatct gccaaaccat gaacctcgcg gctgcccgcg
     9781 cggtgccagc tactcctggt atctttacag tgccaaccgc ctgaaatacc cgatgatgcg
     9841 caaacgcctg atgaaaatgt ggcgtgaagc gaaggcgctg catagcgatc cggttgaggc
     9901 atgggcttct atcattgaag acgccgataa agcgaaaagc tttaagcagg cgcgtggacg
     9961 cggtggattt gttcgttctt cctggcagga ggtgaacgaa ctgatcgccg catctaacgt
    10021 ttacaccatc aaaaactacg gcccggaccg tgttgctggt ttctcgccaa ttccggcaat
    10081 gtcgatggtt tcttacgcat cgggtgcacg ctatctctcg ctgattggcg gtacttgctt
    10141 aagcttctac gactggtact gcgacctgcc tcctgcgtct ccgcaaacct ggggcgagca
    10201 aactgacgta ccggaatctg ctgactggta taactccagc tacatcatcg cctggggttc
    10261 aaacgtgccg cagacgcgta ccccggatgc tcacttcttt actgaagtgc gttacaaagg
    10321 caccaaaact gttgccgtca caccagacta cgctgaaatc gccaaactgt gcgatctgtg
    10381 gctggcaccg aaacagggca ccgatgcggc aatggcgctg gcgatgggcc acgtaatgct
    10441 gcgtgaattc cacctcgaca acccaagcca gtatttcacc gactatgtgc gtcgctacac
    10501 cgacatgccg atgctggtga tgctggaaga acgcgacggt tactacgctg caggtcgtat
    10561 gctgcgcgct gctgatctgg ttgatgcgct gggccaggaa aacaatccgg aatggaaaac
    10621 tgtcgccttt aataccaatg gcgaaatggt tgcgccgaac ggttctattg gcttccgctg
    10681 gggcgagaag ggcaaatgga atcttgagca gcgcgacggc aaaactggcg aagaaaccga
    10741 gctgcaactg agcctgctgg gtagccaaga tgagatcgct gaggtaggct tcccgtactt
    10801 tggtggcgac ggcacggaac acttcaacaa agtggaactg gaaaacgtgc tgctgcacaa
    10861 actgccggtg aaacgcctgc aactggctga tggcagcacc gccctggtga ccaccgttta
    10921 tgatctgacg ctggcaaact acggtctgga acgtggcctg aacgacgtta actgtgcaac
    10981 cagctatgac gatgtgaaag cttatacccc ggcctgggcc gagcagatta ccggcgtttc
    11041 tcgcagccag attattcgca tcgcccgtga atttgccgat aacgctgata aaacgcacgg
    11101 tcgttcgatg attatcgtcg gtgcggggct gaaccactgg tatcacctcg atatgaacta
    11161 tcgtggtctg atcaacatgc tgattttctg cggctgtgtc ggtcagagcg ggggcggctg
    11221 ggcgcactat gtaggtcagg aaaaactgcg tccgcaaacc ggctggcagc cgctggcgtt
    11281 tgcccttgac tggcagcgtc cggcgcgtca catgaacagc acttcttatt tctataacca
    11341 ctccagccag tggcgttatg aaaccgtcac ggcggaagag ttgctgtcac cgatggcgga
    11401 caaatcccgc tataccggac acttgatcga ctttaacgtc cgtgcggaac gcatgggctg
    11461 gctgccgtct gcaccgcagt taggcactaa cccgctgact atcgctggcg aagcggaaaa
    11521 agccgggatg aatccggtgg actatacggt gaaatccctg aaagagggtt ccatccgttt
    11581 tgcggcagaa caaccagaaa acggtaaaaa ccacccgcgc aacctgttca tctggcgttc
    11641 taacctgctc ggttcttccg gtaaaggtca tgagtttatg ctcaagtacc tgctggggac
    11701 ggagcacggt atccagggta aagatctggg gcaacagggc ggcgtgaagc cggaagaagt
    11761 ggactggcag gacaatggtc tggaaggcaa gctggatctg gtggttacgc tggacttccg
    11821 tctgtcgagc acctgtctct attccgacat cattttgccg acggcgacct ggtacgaaaa
    11881 agacgacatg aatacttcgg atatgcatcc gtttattcac ccgctgtctg cggcggtcga
    11941 tccggcctgg gaagcgaaaa gcgactggga aatctacaaa gccatcgcga agaaattctc
    12001 cgaagtgtgc gtcggccatc tgggtaaaga aaccgacatc gtcacgctgc ctatccagca
    12061 tgactctgcc gctgaactgg cgcagccgct ggatgtgaaa gactggaaaa aaggcgagtg
    12121 cgacctgatc ccaggtaaaa ccgcgccaca cattatggtc gtagagcgcg cttatccggc
    12181 gacttacgaa cgctttacct ctatcggccc gctgatggag aaaatcggta atggcggtaa
    12241 agggattgcc tggaacaccc agagcgagat ggatctgctg cgtaagctca actacaccaa
    12301 agcggaaggt ccggcgaaag gccagccgat gctgaacacc gcaattgatg cggcagagat
    12361 gatcctgaca ctggcaccgg aaaccaacgg tcaggtagcc gtgaaagcct gggctgccct
    12421 gagcgaattt accggtcgtg accatacgca tctggcgctg aataaagaag acgagaagat
    12481 ccgcttccgc gatattcagg cacagccgcg caaaattatc tccagcccga cctggtctgg
    12541 tctggaagat gaacacgttt cttacaacgc cggttacacc aacgttcacg agctgatccc
    12601 atggcgtacg ctctctggtc gtcagcaact gtatcaggat caccagtgga tgcgtgattt
    12661 cggtgaaagc ctgctggttt atcgtccgcc gatcgacacc cgttcggtga aagaagtgat
    12721 aggccagaaa tccaacggca acccggaaaa agcgctcaac ttcctgacgc cgcaccagaa
    12781 gtgggggatc cactccacct acagcgacaa ccagttgatg ctgaccttag gtcgcggtgg
    12841 cccggtggtc tggctgagcg aagccgatgc caaagagctg ggcattgccg ataacgactg
    12901 gattgaagtt tacaacagca acggtgcgct gactgcccgt gcggttgtca gccagcgtgt
    12961 tccggcaggg atgaccatga tgtaccacgc gcaggaacgt atcgttaacc tgccgggttc
    13021 ggaaattact ggccagcgtg gggggatcca caactcggtc acccgtatca cgccgaaacc
    13081 gacgcatatg attggtggct atgcccatct ggcatacggc tttaactact acggcaccgt
    13141 aggttctaac cgcgatgagt ttgttgtagt gcgtaagatg aagaacattg actggttaga
    13201 tggcgaaggc aatgactagg tacaggagag cgtaaaatga aaattcgttc acaagtcggc
    13261 atggtgctga atctcgataa gtgcatcggc tgccacacct gttcagttac ctgtaaaaac
    13321 gtctggacca gccgtgaagg cgtggagtac gcgtggttca acaacgtgga aaccaagccg
    13381 ggtcagggct tcccgactga ctgggaaaac caggaaaaat acaaaggcgg ctggatccgt
    13441 aaaatcaacg gcaaactgca gccgcgcatg ggtaaccgtg ccatgttgct gggtaaaatc
    13501 ttcgctaacc cgcatctgcc ggggatcgac gattattacg agccgttcga ttttgactat
    13561 cagaacctgc ataccgcgcc ggaaggcagc aaatcgcagc cgattgcccg tccccgttcg
    13621 ctgattaccg gggaacggat ggcgaaaatc gaaaaagggc cgaactggga agatgacctg
    13681 ggtggtgagt ttgacaaact ggcgaaagac aagaacttcg acaacatcca gaaggcgatg
    13741 tatagccagt tcgaaaacac ctttatgatg tatttgccgc gcctgtgcga acactgcctg
    13801 aacccggcat gtgtggcgac ctgcccgagc ggtgcgattt acaagcgtga agaagatggc
    13861 atcgtcctga tcgaccagga taaatgccgt ggctggcgta tgtgcatcac cggatgcccg
    13921 tacaaaaaaa tctacttcaa ctggaagagc ggtaagtctg agaagtgcat cttctgctat
    13981 ccgcgtattg aagcgggtca gccgaccgtg tgctcagaaa cctgtgtcgg tcgtatccgt
    14041 tatcttggcg tgctgctgta cgatgccgac gcgattgaac gtgcagccag caccgagaac
    14101 gagaaagatc tttaccagcg tcagctggag gtgttcctcg atccgaacga tccgaaagtc
    14161 atcgagcagg cgattaaaga cggtattccg ctgagcgtta ttgaagccgc acagcagtcg
    14221 ccggtttata aaatggcaat ggaatggaaa ctggcgctgc cgctgcatcc agaatatcgc
    14281 acactgccga tggtctggta cgtgccgcct ctgtctccga ttcagtctgc agcagacgcg
    14341 ggtgagctgg gtagcaacgg cattctgcca gacgtcgaaa gcttgcgtat tccggtacag
    14401 tatctggcga atctgctgac cgccggtgat accaaaccgg tactgcgcgc actgaaacgt
    14461 atgctggcga tgcgtcatta caaacgtgct gaaaccgttg acggtaaagt tgatacccgt
    14521 gcgctggaag aggtcggtct gaccgaagcc caggcacagg agatgtaccg ttatctggct
    14581 attgctaact acgaagatcg ctttgtggtg ccgagtagtc atcgtgaact ggcacgggaa
    14641 gccttcccgg agaaaaatgg ctgcggcttt acctttggtg atggctgcca cggctcagat
    14701 accaaattca atctgttcaa cagccgtcgt atcgatgcca tcgatgtgac cagcaaaacg
    14761 gagccgcatc catgatcgaa ctcgtgattg tatcgcgtct ccttgaatat ccggatgctg
    14821 ccttatggca gcatcaacaa gagatgtttg aggtgattgc tgcgtcgaaa aatctgtcga
    14881 aagaagatgc ccatgcgctg ggcattttcc tgcgcgattt aacggcgatg gatccgctcg
    14941 atgcccaggc ccagtacagc gaactgttcg accgtggccg cgccacgtca ctgttgctat
    15001 ttgaacatgt tcacggtgaa tcccgtgacc gcggccaggc gatggtggac ctgctggcgc
    15061 agtacgagca gcacggcttg cagttaaaca gccgcgaatt accggaccat ctgccgctgt
    15121 atctggagta cctgtcgcag ctgccgcaaa gcgaagccgt ggaaggtttg aaagatatcg
    15181 cgccgattct ggcattgctg agcgcgcgtc tgcaacagcg tgaaagccgt tatgccgtga
    15241 tgtttgatct gctgctgaaa ttggccaata ccgctatcga cagcgacaaa gtggcggaaa
    15301 aaattgccga cgaagcgcgc gatgatacgc cgcaggcgct ggatgctgtc tgggaagaag
    15361 agcaggttaa attctttgct gacaaaggct gcggtgattc agcaatcact gctcatcagc
    15421 gtcgctttgc cggtgccgtc gcgccgcaat atctgaatat caccaccgga ggacagcact
    15481 aatgcaattc ctgaatatgt tcttctttga tatctacccg tacatagccg gggcggtctt
    15541 cctgattggt agctggctgc gttatgacta cgggcagtac acctggcgcg cggcgtccag
    15601 ccagatgctg gatcgcaaag ggatgaacct ggcgtcgaac ctgttccata tcggtattct
    15661 ggggattttt gctggtcact tcttcggtat gctgacgccg cactggatgt atgaagcctg
    15721 gctgccgatt gaagtgaaac agaaaatggc gatgtttgct ggtggtgcca gcggcgtgct
    15781 gtgtctgatt ggcggcgtgc tgttgctgaa gcgtcgtctg ttcagcccac gcgtgcgtgc
    15841 aaccactacc ggagcggata tcctgatcct gtcgctgctc gttatccagt gcgcgctggg
    15901 cctgttgacc attccgttct ccgctcagca tatggacggt agcgagatga tgaaactggt
    15961 tggctgggcg cagtcggtgg tgaccttcca cggtggcgct tctcaacacc tcgatggtgt
    16021 ggcgtttatc ttccgtctgc acctggtgct ggggatgacg ttattcctgc tgttcccgtt
    16081 ctcgcgtctg gtacacatct ggagcgtacc ggtggagtat ctgacacgta agtaccagct
    16141 ggtgcgcgct cgtcactaaa cgaattttag ttcacataga ccctgcttcg gcggggtttt
    16201 ttatgggcac ggtgcggggt gagttgtcgg atgcgcttcg cttatccgac ctacagggga
    16261 ggatattgta ggcccggtaa acgtggtgcc atcgggcaaa attagctcgg agtaacaggt
    16321 tttgattatt caaggaggcg atggtggtgg gggaaggatt actcagcgct gcgcgcttcg
    16381 cccttcgggt cgttgcctgc ggcaacgctc tctcgctggc gctcaagtcg aaccttggtc
    16441 gaagcttctc atccttcccc gcttgggcag aatatttaat tgcggattcg tttgagagtt
    16501 tacggacttt tgaaaagtga tggtggtggg ggaaggattc gaaccttcga agtcgatgac
    16561 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccagggg taattcaaat
    16621 tttgaggtaa tgcttgagat ggtggtgggg gaaggattcg aaccttcgaa gtcgatgacg
    16681 gcagatttac agtctgctcc ctttggccgc tcgggaaccc caccacgggg taatgctttt
    16741 tactggcctg ctcccttatc gggaagcggg gcgcatcata tcaaatgacg cgccgctgta
    16801 aagtgttacg ttgagaaaaa tgaactggtt gcgtaatttt catccgtaac ggattaaagg
    16861 taaccagtta tttttgctgg cgattaaaga ataatcgtcc gattaccgta aacaaagacg
    16921 cgctgtgcca gtactttgta tagcgcacga cttaagacgt ttttctcgac gtcacgacct
    16981 gcgcgcatca tatcttcagc tgtgtaggta tgatcgacat gaataacgtc ctgcatgatg
    17041 attgggcctt cgtccagatt gtcattcaca tagtgagcgg ttgcgccaat aatcttcaca
    17101 ccacgttcat aggcctggtg ataaggacgt gcgccgataa acgcaggcag gaatgaatgg
    17161 tgaatattga tgattttatt cgggaagcgt gccacaaatt ccggcgttaa tacccgcata
    17221 tacttcgcca gcaccacata gtcaggttta taagcatcaa tggcgtctgc catcttttga
    17281 tcgtgctcgt tgcggcttaa cccttcatgg tttaccagct caaacggaat atcaaaacgc
    17341 tcaaccagag aacgtaaagt atcgtggtta ccaataactg ccgcgatttc gacatccagg
    17401 ccgccgtaat tggctttcat caacaaatcg ccaaggcaat gcgcttcttt agtgaccaga
    17461 atcactatcc ggcgacgacc ggcaggattc agctcacgca cggagccttc tggcaatgcg
    17521 ctatcgagat ccgccagcag ggtggaatca ttaaaaatcc cttccagttc cgtgcgcata
    17581 aaaaagcgcc cggtacggtg atcaacaaat tcattgttct gtacgatatt taactcgtgc
    17641 ttgtagcaaa tattggtaat acgtgcgatc agaccttttt ggtccggaca aatagtacgc
    17701 agaactttac gttggagtga atgcattgct ggaaaaacct tgttgagagt gtttgctaaa
    17761 ccgtaccgtc aaccattatt ggccgcagca ctttttaaat tttttacctg aaccacaagg
    17821 gcagggatcg ttgcgaccaa actgcggacg tgtaccgtca atatagtacc attgaccgtt
    17881 ttctttcaaa aatcgcgaac gttcaattat tgcacctgtt ttgccacctt cggtaaatcg
    17941 agcaacaaaa ctgacaaatc cgatattgtc ggcgtcctgc caacaatgtt caaatacggt
    18001 caatcccaac cattcagtat gcgcaaatcc ggccatcaat tcggcacgta acgccgctgc
    18061 tccacaagag ggatgccagg tctttattaa ataatctgcg tcttgcatca caaaagcgca
    18121 gtaacgcgaa cgcatgagat gttctggatc aggtgcaacc ttttcaccag acacataagg
    18181 gtggcaacat aggctatact cgacagcact accacaggga caaagctgag acacaaataa
    18241 tctccctgga aacaataacg gcgtattaac cgcctgagta gcactatgtt aaccgagcag
    18301 tagcgatgtg gctacgattg cattccaggg gaatcttgcg ggaataatga gaaagataaa
    18361 aatagggctg gcgctgggat ctggcgcggc gagaggttgg tcgcatattg gcgttattaa
    18421 tgcgctaaaa aaagtgggta ttgaaattga tatcgttgca ggatgttcaa ttggttcgct
    18481 ggtgggcgct gcctatgcat gcgatcgatt atctgcgctg gaagattggg tgacctcttt
    18541 cagttattgg gatgttttac gcctgatgga tctctcctgg cagcgcggtg ggttactgcg
    18601 cggcgagcgt gtcttcaatc aatgccgcga aataatgccg gaaacagaga tcgaaaattg
    18661 ttcccgtcgc tttgcggctg ttgccaccaa tttaagtacg ggacgtgaat tatggtttac
    18721 tgaaggcgat ctccatcttg ctattcgcgc atcatgcagt attccaggac tcatggcacc
    18781 tgttgcacat aacggctact ggctggttga tggagcagta gttaacccaa ttcctatttc
    18841 cctcacgcgt gtattggggg ctgatattgt gatagcggtc gacctgcagc acgatgctca
    18901 tttgatacaa caagatttgc tctcctttaa tgtcagtgaa gaaaatagcg agaatggtga
    18961 atctttgccg tggcatgcgc gtctgaaaga aaggttaggc agtataacaa cacgtcgggc
    19021 ggtgacagcg ccaacggcaa cagagattat gaccacttct atccaggtgc tggagaaccg
    19081 ccttaaaagg aaccgcatgg caggtgatcc gcccgatatt ctgattcaac ctgtttgccc
    19141 gcaaatatct acgcttgatt tccatcgcgc gcacgctgcc attgcggccg gacagctggc
    19201 agtggaaagg aaaatggacg aacttttgcc gttggtacgc accaacattt gaccagaatt
    19261 tttatctaca cttaagttaa ttctgacagg cgcaggtggc aatagcatgc cactattgag
    19321 taaagccagt caggggagag aacatgacgc agccattggt cggaaaacag attctcattg
    19381 ttgaagatga gcaggtattt cgctcgcttc tggattcatg gttttcctca ttgggagcga
    19441 caacggtact ggcggctgat ggggtggatg cccttgagtt gctgggaggt ttcactccag
    19501 acctgatgat atgtgatatc gcgatgccac gaatgaacgg gcttaaactg ctggagcata
    19561 tacgtaacag aggcgaccag accccagttc tggtgatatc tgccactgaa aatatggcag
    19621 atattgccaa agcgttacgt ctgggcgttg aagatgtttt gctgaaacca gttaaagatc
    19681 tgaatcgctt gcgcgagatg gtttttgcct gtctctatcc cagcatgttt aattcgcgcg
    19741 ttgaggaaga ggaaaggctt tttcgcgatt gggatgcaac ggttgataac cctgccgcag
    19801 cggcgaaatt attacaggaa ctacaaccgc cggttcagca ggtgatttcc cattgccggg
    19861 ttaattatcg tcaattggtt gccgcggaca aacccggcct ggtgcttgat attgccgcac
    19921 tttcggaaaa cgatctggca ttttattgcc ttgatgtcac ccgagctgga cataatggcg
    19981 tacttgctgc cttgttatta cgcgcattgt ttaacggatt attacaggaa cagcttgcac
    20041 accaaaatca acggttgcca gagctgggcg cgttattgaa gcaggtaaac catttactcc
    20101 gtcaggccaa tctgccgggg cagtttccgc tattagttgg ctattatcat cgcgaactga
    20161 aaaatctcat tctggtttct gcgggtctga atgcgacgtt aaataccggc gaacaccagg
    20221 tgcaaatcag taatggtgtt ccgttaggca ctttaggtaa cgcttatttg aatcaattga
    20281 gccagcgatg cgatgcctgg caatgccaaa tatggggaac cggtggtcga ctgcgcttga
    20341 tgttgtctgc agaatgagca aacgataacg cggactaaat ttgcattacc tgctaatgtc
    20401 ggctggtggt actatcgtcg ccattcgtat aagtaattgt cttaattatg ctaactcgcc
    20461 tccttttcag aacttagccc cttcggggtg ctgatatact gggatgcgat acagaaatat
    20521 gaacacgttc aaaacacgaa cagtccagga gaatttaaat ggctgccatt aatacgaaag
    20581 tcaaaaaagc cgttatcccc gttgcgggat taggaaccag gatgttgccg gcgacgaaag
    20641 ccatcccgaa agagatgctg ccacttgtcg ataagccatt aattcaatac gtcgtgaatg
    20701 aatgtattgc ggctggcatt actgaaattg tgctggttac gcactcatct aaaaactcga
    20761 ttgaaaacca ctttgatacc agttttgaac tggaagcaat gctggaaaaa cgtgtaaaac
    20821 gtcaactgct tgatgaagtg cagtctattt gtccgccgca cgtgactatt atgcaagttc
    20881 gtcaggggct ggcgaaaggc ctgggacacg cggtattgtg tgctcacccg gtagtgggtg
    20941 atgaaccggt agctgttatt ttgcctgatg ttattctgga tgaatatgaa tccgatttgt
    21001 cacaggataa cctggcagag atgatccgcc gctttgatga aacgggtcat agccagatca
    21061 tggttgaaca ggttgctgat gtgaccgcat atggcgttgt ggattgcaaa ggcgttgaat
    21121 tagcgccggg tgaaagtgta ccgatggttg gtgtggtaga aaaaccgaaa gcggatgttg
    21181 cgccgtctaa tctcgctatt gtgggtcgtt acgtacttag cgcggatatt tggccgttgc
    21241 tggcaaaaac ccctccggga gctggtgatg aaattcagct caccgacgca attgatatgc
    21301 tgatcgaaaa agaaacggtg gaagcctatc atatgaaagg gaagagccat gactgcggta
    21361 ataaattagg ttacatgcag gccttcgttg aatacggtat tcgtcataac acccttggca
    21421 cggaatttaa agcctggctt gaagaagaga tgggcattaa gaagtaacat ccatatcggt
    21481 gttatccacg aaacggcgtt gaacaatcga cgccgttttt ttatagctta ttcttattaa
    21541 attgtcttaa accggacaat aaaaaatccc gccgatggcg ggatttttag caattgcaat
    21601 ctacaaaaga ttattgcttg atcaggaaat cgtcgaggga tttaccttgc tcatccattg
    21661 cttttttgat tacagctgga gtacggccct ggccagtcca ggttttagtt tcgccgtttt
    21721 cgtcaacata gctatatttt gccggacgct gagcacgttt agctttggtg ccggatttaa
    21781 cggcagcaag gctattcagc agttcgttcg ggtcaatacc gtcagcgatc agcatttcgc
    21841 gatattgctg cagtttacga gtgcgctctt caacttcagc agcagccgcg ctttcttctt
    21901 cgcgacgttc gttaacaaca acttctaatt tttccagcat ttcttccagc gtttcaagtg
    21961 tacattctct tgcctgcgca cgaagagtac ggatgttgtt cagaatttta agtgcttcgc
    22021 tcattgtagt aatctcaaac ttatattggg gtggtttgtt gaggtaataa tagagcctta
    22081 aattcagttg tgcaatagcc aggaatgtaa ggaattcaaa attgttcttt attttgtgcc
    22141 gccaataaat atcttttcat aaaattagcc agaaaagacg cggcatatag ccctatttac
    22201 accgatgatt tcgcagcacg tgaggttaaa acttcctgat tcatgtcaca ttttatgggg
    22261 agattatcgt aggctgacgg cctttcagtc ttctgtatta gttgtgttta cgagaattcc
    22321 ctattaagcg aatgatgaaa agtagaacag tcgcaataag agcatggact tagtattgca
    22381 ctatctcctg gaggtcaaca gagggctatt acttgcgcaa caggttaaag attgtgaata
    22441 gttaccagca gtcatttacc cgcttataac aagcgaggca gttgtaatga tagctcagaa
    22501 ggattatgca aggcttcgta agggagaacg catataccca cttctgtgca tactgttgag
    22561 ctgaaaaact gacgaattat gataaactcc agccaacttt atttcatatc attgagggcc
    22621 tgtggctgat ggcacagcta tatttctact attccgcaat gaatgcgggt aagtctacag
    22681 cattgttgca atcttcatac aattaccaag aacgcggcat gcgcactgtc gtatatacgg
    22741 cagaaattga tgatcgcttt ggtgccggga aagtcagttc gcgtataggt ttgtcatcgc
    22801 ctgcaaaatt atttaaccaa aattcatcat tatttgatga gattcgtgcg gaacatgaac
    22861 agcaggcaat tcattgcgta ctggttgatg aatgccagtt tttaaccaga caacaagtat
    22921 atgaattatc ggaggttgtc gatcaactcg atatacccgt actttgttat ggtttacgta
    22981 ccgattttcg aggtgaatta tttattggca gccaatactt actggcatgg tccgacaaac
    23041 tggttgaatt aaaaaccatc tgtttttgtg gccgtaaagc aagcatggtg ctgcgtcttg
    23101 atcaagcagg cagaccttat aacgaaggtg agcaggtggt aattggtggt aatgaacgat
    23161 acgtttctgt atgccgtaaa cactataaag aggcgttaca agtcggctca ttaacggcta
    23221 ttcaggaaag gcatcgccac gattaataag aatttcttta ctgacagggt gagcagggca
    23281 cttttatcct gtcagttcgt tttacgcact tcttccgggc tatataccct tctcggcagt
    23341 tttttaacgc cgctatacgc ctcacagggc tcttaagcac cgacgttgac ttgtgacctg
    23401 taaagtacaa tatccctgtg tttaggcgtt atacatcgtc gcaaatatga tgaaggctaa
    23461 tgctgtcggt ttatggaaaa gttgctttgg gtaaacaaaa aatacggccc cagaagggca
    23521 atgccgttca cttaagagga gcggcactat gtttcacagg ataacgggtt tttgatatct
    23581 taaccgacct cggccttgat ggtcgggggc gttttgttat gaacaccact tccagagcac
    23641 cccgaagatg ctccagtcgt ttcgggatgg tccctggtga cgccgtgttt cccagctcta
    23701 tcatttctga tgcgatattc ttccacgcag gcagtagcca gtggcggtta caacccttct
    23761 ggttcagcgt tagcagcagg tcttcgctgt aaaaaagctt atcaaacaac gtaatagagt
    23821 tatccgggat ggtggcgagc atggagtggg ccagcacagt ttcgctctgc cggtaaggtg
    23881 cggtcacggc attcagcaga atgtgacttc ccaggttcat taaggccacc agacgcataa
    23941 ccgggtaggc gttctgccgc ttagtggatg tgttggcaga cccataatat tcacgcagct
    24001 cgggtttatc aggtgtcctg aactgtgcgc catcaatggc aaaaagttgc aggccgtgcc
    24061 agtcatcctt caggtaacgt tccgcgcccc tgtctgtgcg gtctggcgga agagccattc
    24121 cactggggcg gcccccacgc gctgacgcgc ttgggtgaca gcgctgcggg ccagcaggtt
    24181 catccccgct tcgccatccg cgctcaggtt cagacggcga acaacatcgg taattggctc
    24241 attgcaccac catccagata accatgtccc ccggtaaacg acggcggcga acggtcgcat
    24301 gagcagaaag cgtcaggcag tgttgtatcc actcggtggg aaggtgttct gcaaatagtt
    24361 gtgcagaggg cggaggcata agcggatggt cactgaaatc gagcagatca ttgagaagtg
    24421 gcataagaaa acggctccct attgtggaag ccgttatagt gcctcagttt aaggatcggt
    24481 caactaatcc ttaactgatc ggcattgccc agaaggggcc gtttatgttg ccagacagcg
    24541 ctactgatta agcggatttt ttcgcttttt tctcagcttt agccggagca gcttctttct
    24601 tcgctgcagt ttcaccttct acataatcac gaccgtagta ggtatccagc agaatctgtt
    24661 tcagctcgga gatcagcggg taacgcgggt tagcgccggt gcactggtca tcgaatgcat
    24721 cttcagacag tttatccacg ttcgccagga agtctgcttc ctgaacgcca gcttcacgga
    24781 tagatttcgg aatacccagt tcagctttca gcgtttccag ccatgccagc agtttctcga
    24841 tcttagcagc agtacggtcg cccggtgcgc tcagacccag gtggtcagca atttcagcat
    24901 aacgacggcg agcctgcgga cggtcatact ggctgaatgc agtctgcttg gtcgggttgt
    24961 cgttcgcgtt gtagcgaata acgttacaaa tcagcagggc gtttgccaga ccgtgcggaa
    25021 tatggaactg ggaacccagt ttgtgcgcca ttgagtgaca tacacccagg aaggcgttcg
    25081 caaacgcgat acccgcgata gtcgctgcac tgtgaacacg ttcacgcgct accggatttt
    25141 tagacccttc gtggtaggac gctggcagat attctttcag cagtttcagt gcctgcagag
    25201 cctgaccatc agagaactca gatgccagta cagaaacata agcttccatg gcgtgagtta
    25261 ctgcgtccag accaccgaaa gcacacaggg acttcggcat gtccataacc aggttggcgt
    25321 cgacaatcgc catatccgga gtcagcgcat agtctgccag cggatatttc tgaccagtag
    25381 cgtcgtcagt tacaaccgca aacggagtga cttcagaacc tgtaccagaa gtggtggtga
    25441 cagcgatcat tttcgctttc acgcccattt tcgggaactt gtagatacgt ttacggatat
    25501 ccataaagcg cagcgccagc tcttcgaagt gagtttccgg atgttcgtac ataacccaca
    25561 tgatcttcgc ggcgtccatc ggggaaccac cacccagcgc gataatcacg tctggtttga
    25621 aggagtttgc cagttctgca cctttacgaa cgatgctcag ggtcgggtcc gcttctactt
    25681 cgaagaagac ttcagtttca acgcctgctg ctttcagtac ggaagtgatc tgatcagcat
    25741 aaccattgtt gaacaggaag cggtcagtca cgatgagcgc acgtttgtgg ccatcagtaa
    25801 tcacttcatc cagcgcgatt ggcagggagc cacggcggaa gtagatagat ttcggaagtt
    25861 tgtgccacaa catgttttca gctcgcttag caacggtttt cttgttgatc aggtgtttcg
    25921 gaccaacgtt ttcagagatg gagttaccac cccaagaacc acaacccaga gtcagggaag
    25981 gtgcgagttt gaagttatac aggtcaccga taccaccctg agacgctggg gtgttaatca
    26041 ggatacgagc cgttttcatt ttctgaccga agtaagaaac gcgagccggt tggttatcct
    26101 ggtcagtgta caggcaagag gtatgaccga taccgcccat agcaaccagt ttctctgctt
    26161 tttctaccgc gtcttcgaaa tctttagcgc ggtacattgc cagagtcggg gacagttttt
    26221 catgtgcgaa cggttcgctt tcatcaacaa cggtcacttc accgatcaga atcttggtgt
    26281 tttctggtac agagaagcct gccagttcag caattttata ggctggctga ccaacgatag
    26341 ccgcgttcag cgcaccgttt ttcaggataa catcctgaac agctttcagc tctttaccct
    26401 gcaacagata gccgccgtgg gttgcaaaac gttcacgtac agcgtcataa acagagtcaa
    26461 caacaacaac agactgttca gaagcacaga ttacgccgtt gtcgaaggtt ttggacatca
    26521 gtacagatgc aactgcacgt ttgatatcag cagtttcatc gataacaact ggagtgttgc
    26581 ccgcgcctac accgatagct ggtttaccgg agctgtatgc ggctttaacc atgcccggac
    26641 caccagtcgc gaggatcagg ttgatgtctg ggtggtgcat cagtgcgtta gacagttcaa
    26701 cagaaggttg atcgatccag ccgatcagat ctttcggagc accggcagcg atagcagcct
    26761 gcagaacgat atcagccgct ttgttggtgg catcttttgc acgcgggtgc ggggagaaga
    26821 taatggcgtt acgggtcttc agactgatca gcgatttgaa gatagcagtt gaagtcgggt
    26881 tagtggtcgg aacgataccg caaataatac cgattggttc agcgatagtg atggtaccaa
    26941 aagtgtcgtc ttcagacaga ataccacagg ttttttcatc tttataggcg ttgtagatat
    27001 attcagaagc aaagtggttt ttgatcactt tatcttcgac gatacccatg ccggattcgg
    27061 caacggccat tttcgcgagt gggattcgag catctgcagc agccagagcg gcggcgcgga
    27121 agattttgtc tacttgctct tgagtgaaac tggcatattc acgctgggct ttctttacac
    27181 gctctacgag tgcgttaagt tcagcgacat tagtaacagc cataatgctc tcctgataat
    27241 gttaaacttt tttagtaaat catctgctcg aatacgagag tatagtcagt gcggtgatga
    27301 tttgcttaac ctatgaaaat caaaagctta ctcgcgctca cactcactgt gatttactaa
    27361 aagagtttaa acattagagt tattatctct aatgcgtcac ttccaggtgg cgtaagcaag
    27421 attactcact tctgggtact gattacgtga tccaaatcaa attttttgca aagctgacac
    27481 ctttcagcat cgcttttcgc cattatagct aacagttaat aaattgtagt atgatttggt
    27541 ggctacatta gcatgttttg cacaactaga taacaataac gaatgatagc aattttaagt
    27601 agttaggagg tgaaaaatgc tgtcaaaagg cgtattgtca gcgcgtcttt tcaaccttat
    27661 ttatggctaa cattatccgg cttttgcttc ggagctaacc gtgattcaga ccttttttga
    27721 ttttcccgtt tacttcaaat ttttcatcgg gttatttgcg ctggtcaacc cggtagggat
    27781 tattcccgtc tttatcagca tgaccagtta tcagacagcg gcagcgcgaa acaaaactaa
    27841 ccttacagcc aacctgtctg tggccattat cttgtggatc tcgctttttc tcggcgacac
    27901 gattctgcaa ctttttggta tatcaattga ttcgttccgt atcgccgggg gcatcctggt
    27961 ggtgacgata gcgatgtcga tgatcagcgg caagcttggc gaggataaac agaacaagca
    28021 agaaaaatca gaaaccgcgg tacgtgaaag cattggtgtg gtgccattgg cgttgccgtt
    28081 gatggcgggg ccaggggcga tcagttctac catcgtctgg ggtacgcgtt atcacagcat
    28141 tagctatctg tt
//
LOCUS       DAJTOR010000036        27782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTOR010000036 DAJTOR010000000
VERSION     DAJTOR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.036"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..528
                     /locus_tag="OO249_001396"
     CDS             <1..528
                     /locus_tag="OO249_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5635746.1"
                     /translation="PKYKIMANHHGIIFDEQRKRYIIGSITVSIDEMTNALDPVELAE
                     IINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAH
                     NGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNR
                     NGLMPLIRRRTMLQL"
     gene            894..1961
                     /locus_tag="OO249_001397"
     CDS             894..1961
                     /locus_tag="OO249_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5635747.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1958..4693
                     /gene="rbbA"
                     /locus_tag="OO249_001398"
     CDS             1958..4693
                     /gene="rbbA"
                     /locus_tag="OO249_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5635748.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDMWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4693..5817
                     /locus_tag="OO249_001399"
     CDS             4693..5817
                     /locus_tag="OO249_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5635749.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5890..6164
                     /locus_tag="OO249_001400"
                     /pseudo
     CDS             5890..6164
                     /locus_tag="OO249_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6161..6520
                     /locus_tag="OO249_001401"
     CDS             6161..6520
                     /locus_tag="OO249_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5635750.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="OO249_001402"
     CDS             complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="OO249_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5635751.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="OO249_001403"
     CDS             complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="OO249_001403"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5635752.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="OO249_001404"
     CDS             complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="OO249_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5635753.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="OO249_001405"
     CDS             complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="OO249_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5635754.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSH
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="OO249_001406"
     CDS             complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="OO249_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5635755.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQFFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="OO249_001407"
     CDS             complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="OO249_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5635756.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="OO249_001408"
     CDS             complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="OO249_001408"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5635757.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12715..13764)
                     /locus_tag="OO249_001409"
     CDS             complement(12715..13764)
                     /locus_tag="OO249_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5635758.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVTITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMIGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYACILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13896..15113
                     /locus_tag="OO249_001410"
     CDS             13896..15113
                     /locus_tag="OO249_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5635759.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15117..15674)
                     /gene="dcrB"
                     /locus_tag="OO249_001411"
     CDS             complement(15117..15674)
                     /gene="dcrB"
                     /locus_tag="OO249_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5635760.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAENNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15747..16412)
                     /locus_tag="OO249_001412"
     CDS             complement(15747..16412)
                     /locus_tag="OO249_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5635761.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16633..16878
                     /gene="tusA"
                     /locus_tag="OO249_001413"
     CDS             16633..16878
                     /gene="tusA"
                     /locus_tag="OO249_001413"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5635762.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16980..19178)
                     /gene="zntA"
                     /locus_tag="OO249_001414"
     CDS             complement(16980..19178)
                     /gene="zntA"
                     /locus_tag="OO249_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5635763.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEYKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19252..19878)
                     /locus_tag="OO249_001415"
     CDS             complement(19252..19878)
                     /locus_tag="OO249_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5635764.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYF"
     gene            20019..20378
                     /locus_tag="OO249_001416"
     CDS             20019..20378
                     /locus_tag="OO249_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5635765.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20381..20650)
                     /locus_tag="OO249_001417"
     CDS             complement(20381..20650)
                     /locus_tag="OO249_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5635766.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20640..21236)
                     /gene="rsmD"
                     /locus_tag="OO249_001418"
     CDS             complement(20640..21236)
                     /gene="rsmD"
                     /locus_tag="OO249_001418"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5635767.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21386..22879
                     /gene="ftsY"
                     /locus_tag="OO249_001419"
     CDS             21386..22879
                     /gene="ftsY"
                     /locus_tag="OO249_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5635768.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22882..23550
                     /gene="ftsE"
                     /locus_tag="OO249_001420"
     CDS             22882..23550
                     /gene="ftsE"
                     /locus_tag="OO249_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5635769.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23543..24601
                     /gene="ftsX"
                     /locus_tag="OO249_001421"
     CDS             23543..24601
                     /gene="ftsX"
                     /locus_tag="OO249_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5635770.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24846..25700
                     /gene="rpoH"
                     /locus_tag="OO249_001422"
     CDS             24846..25700
                     /gene="rpoH"
                     /locus_tag="OO249_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5635771.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25971..27074
                     /gene="livJ"
                     /locus_tag="OO249_001423"
     CDS             25971..27074
                     /gene="livJ"
                     /locus_tag="OO249_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5635772.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGALKGFEFGVFDWHANGTATDAK"
     gene            complement(27273..27656)
                     /gene="panM"
                     /locus_tag="OO249_001424"
     CDS             complement(27273..27656)
                     /gene="panM"
                     /locus_tag="OO249_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5635773.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6570 a         7215 c         7729 g         6268 t
ORIGIN      
        1 cccaaataca aaataatggc aaatcatcat gggataattt ttgatgagca gcgaaaacgt
       61 tacattatag gatcgataac agtctctatt gatgaaatga ccaatgctct cgatccggtc
      121 gaattagcgg aaattattaa taaaaagcac catgctgtaa ttaatggcaa aaagtttaaa
      181 aatagccggg ctatctcttg tcgatccttt aatcgttatt tttctgtttc gacagattac
      241 agacctaaac ttgaagcact attagcatgc tcacaaatgt taggtataaa tcaagttgtt
      301 gcacataacg gtaatggtgg aagagaatgt ataggtgaaa cggggacagt tttaggtctt
      361 aacgcccgtg acagtaaaca tgctggaaga atgttcagca tgcataattg ccaaattaac
      421 cctggtgcag ggcctgagat aaccactccc tggaagtctt accagcatga aaaaaacaga
      481 aatggactta tgcctttgat tagaaggcgc acaatgttac agctctgaac catagacaac
      541 atgaatatta atgttactat ctggttgaga ctgttgataa aacgtaaaaa ggaatgctct
      601 gttcctgcaa ctgaacaggc gtgaaacgcg atccaacagg atggataccg ttttgtcagc
      661 cagttttata gatcacactt atgagtatgc gtgtttcatt tacggccagg aatgctgatg
      721 atgacaacat tactacccgt tttcactaag ccttccccgt tggcgctcaa tgctctgcgc
      781 gctggtcgta tttgtcgttt ccttcttatc ccggatggaa gaatccgctg aacgtcgttc
      841 ggcggcagtt gtaacacgcg tcgtttgctt acagggatta tggcgggccg attatggata
      901 agagtaagcg ccatctggcg tggtgggttg tcggggcact ggcggtggct gccgtcgtgg
      961 cgtggtggct gttgcgcccg gcgggtgtgc cggaaggctt tgctgtcagc aatgggcgca
     1021 ttgaagcgac ggaagtggat atcgccagca aaattgccgg gcgtatcgac accattctgg
     1081 tgaaagaagg ccagtttgtt cgcgaaggtg aagtgctggc gaagatggat actcgtgtgt
     1141 tgcaggaaca gcgactggaa gccatcgcgc aaatcaaaga ggcacaaagc gccgttgccg
     1201 ccgcgcaggc tttgctggag caacgacaaa gcgaaactcg cgccgcgcag tcgctggtta
     1261 atcaacgcca ggcagaactg gactccgtag caaaacgtca tatgcgttcc cgttcactgg
     1321 cccaacgagg ggctatttct gcgcaacagc tggatgacga ccgcgccgcc gctgagagcg
     1381 cccgagctgc gctggaatcg gcaaaagctc aggtttctgc ttctaaagcc gctatagagg
     1441 cggcacgtac caatatcatt caggcgcaaa cccgcgtcga agcggcacaa gccactgaac
     1501 ggcgcattgc cgcagatatc gatgacagcg aactgaaagc cccgcgtgac ggacgcgtgc
     1561 agtatcgggt tgccgagcca ggggaagtgc tggcggcagg cggtcgggtg ctgaatatgg
     1621 tcgatctcag cgacgtctat atgactttct tcctgccaac cgaacaggcg ggcacgctaa
     1681 aactgggcgg tgaagcacgg ctgatcctcg atgccgcgcc agatctgcgt attccggcga
     1741 ccatcagttt tgtcgccagc gtcgcccagt tcacgccaaa aaccgtcgaa accagcgatg
     1801 aacggctgaa actgatgttc cgcgtcaaag cgcgtatccc accggaatta ctccagcagc
     1861 atctggaata tgtcaaaacg ggcttgccgg gcgtagcgtg ggtgcgggtg aatgaagaac
     1921 ttccgtggcc tgacgacctg gtggtgaggt tgccgcaatg acgcatctgg aactggttcc
     1981 cgtcccgcct gtcgcgcaac tggcgggcgt gagccagcat tatggaaaaa ccgttgcgct
     2041 gaacaatatc actctcgata ttccggcccg ctgtatggtc gggctgattg gcccggacgg
     2101 cgtcgggaag tcgagtttgt tgtcgttgat ttccggtgct cgtgtcattg agcagggcaa
     2161 tgtgatggtg ctgggcggcg atatgcgcga cccgaagcat cgccgcgacg tctgcccgcg
     2221 catcgcctgg atgccgcagg ggctgggcaa aaacctctac cacaccttgt cggtgtatga
     2281 aaacgtcgat tttttcgccc gcctgttcgg tcacgacaaa gcggagcggg aagtgcgaat
     2341 taatgagctg ctgaccagca cggggttagc accgtttcgc gatcgtccgg cagggaaact
     2401 ctccggcggg atgaagcaaa aacttgggct gtgctgcgcg ttaatccacg acccggaact
     2461 gttgatcctt gatgagccaa caacgggggt tgacccgctc tcccgcgccc agttctggga
     2521 tctgatcgac agtattcgcc agcggcagag caatatgagc gtgctggtcg ccaccgccta
     2581 tatggaagag gccgaacgct tcgactggct ggtagcgatg aatgccggag aagtgctggc
     2641 aaccggcagc gccgaagagc tgcggcagca aacgcaaagc gccacgctgg aagaagcatt
     2701 tattaatctg ttaccgcaag cgcaacgcca ggcgcatcag gcggtagtga tcccaccgta
     2761 tcaacctgaa aacgcagaga ttgccatcga agcgcgcgat ctgaccatgc gttttggttc
     2821 cttcgttgcc gttgatcacg tcaatttccg cattccacgc ggggagattt ttggattcct
     2881 tggttcgaac ggctgcggta aatccaccac catgaaaatg cttaccggac tgctgcccgc
     2941 cagcgaaggt gaggcgtggc tatttgggca accggttgat ccaaaagata tcgatacccg
     3001 ccgtcgtgtg ggctatatgt cgcaggcgtt ttcgctctat aacgaactca ccgtgcggca
     3061 aaacctggag ttacatgccc gtttgtttca catcccggaa gcggaaattc ccgcgcgagt
     3121 ggctgaaatg agcgagcgtt ttaagctcaa cgacgttgaa gatgttctgc cggagtcatt
     3181 gccgctcggc attcgccagc ggctttcgct ggcggtggcg gtgattcatc gcccggagat
     3241 gttaatcctc gatgagccta cttctggtgt cgatccggtg gcgagggata tgttctggca
     3301 gttgatggtc gatctctcgc gccaggacaa agtgactatc ttcatctcca cccactttat
     3361 gaacgaagcg gaacgttgcg accgcatctc actgatgcac gccggaaaag tgcttgccag
     3421 cggtacaccg caggaactgg ttgagaaacg cggagccgcc agtctggaag aggcatttat
     3481 cgcctatttg caggaagcgg cagggcagag caacgaagcc gaagcgccgc ccgtggtaca
     3541 cgacaccacc cacgcgccgc gtcagggatt tagcctgcgc cgtctgttta gctacagccg
     3601 ccgcgaagcg ctggaactgc gacgcgatcc agtacgttcg acgctggcgc tgatgggaac
     3661 ggtgatcctg atgctgataa tgggttacgg catcagtatg gatgtggaaa acctgcgctt
     3721 tgcggtgctc gaccgcgacc agaccgtcag tagccaggcg tggacactca acctctccgg
     3781 ttcccgttac tttatcgaac agccgccgct caccagttat gacgagcttg atcgtcggat
     3841 gcgtgcgggc gatatcacgg tggcgattga gatcccgccc aatttcgggc gcgatatcgc
     3901 gcgtggtacg cctgtggaac tcggcgtctg gatcgacgga gcgatgccca gtcgtgccga
     3961 aacggtaaaa ggttacgtgc aggcaatgca ccagagttgg cttcaggatg tggcgagccg
     4021 acaatcgaca cccgccagcc aaagcgggct gatgaacatt gagacgcgtt atcgctataa
     4081 cccggacgta aaaagcctgc cagcaattgt tccggcggtg atcccgcttc tgctgatgat
     4141 gatcccgtca atgctaagca cccttagcgt ggtgcgggaa aaagagcttg ggtcgattat
     4201 caacctttac gtgaccccca ccacgcgtag tgaatttttg cttggtaaac agttgccata
     4261 catcgcgctg gggatgctga actttttcct gctctgcggc ctgtcggtgt ttgtgtttgg
     4321 cgtaccgcat aaaggcagtt tcctgacgct caccctggcg gcgctgctgt atatcatcat
     4381 tgccaccgga atggggctgc tgatctccac ctttatgaaa agccagattg ccgccatttt
     4441 cggaacggcg attatcacgt tgatcccggc gacacagttt tccgggatga tcgatccggt
     4501 agcttcgctg gaagggcctg gacgttggat cggcgaggtt tacccgacca gtcattttct
     4561 gactatcgcc cgcgggacgt tctcgaaagc gctggatctg actgatatgt ggcaactttt
     4621 tatcccgttg ctgatagcca tcccgctggt gatgggctta agtatcctgc tgctgaaaaa
     4681 acaggaggga tgatgcgcca tttacgcaat atttttaatc tgggtatcaa agagttgcgc
     4741 agtctgctcg gtgataaagc gatgctgacg ctgattgtct tctcgtttac ggtgtcggtg
     4801 tattcgtcag cgaccgttac gccaggatcg ttgaacctcg cgccgatcgc cattgccgat
     4861 atggatcaat cgcagttatc gaaccggatc gttaacagct tctatcgtcc gtggtttttg
     4921 ccaccggaga tgatcaccgc cgatgagatg gatgccggac tggacgccgg acgctatacc
     4981 ttcgcgataa atattccgcc taattttcag cgtgatgtcc tcgccggacg ccagccggat
     5041 attcaggtga acgtcgatgc cacgcgcatg agccaggcat ttaccggcaa tgggtatatc
     5101 cagaatatta tcaacggtga agtgaacagc tttgtcgcgc gctaccgtga taacagcgaa
     5161 ccgttggtat cgctggaaac ccggatgcgc tttaacccga accttgatcc cgcgtggttt
     5221 ggcggggtga tggcgatcat caacaacatt accatgctgg cgattgtatt gaccggatcg
     5281 gcgctgatcc gcgagcgtga acacggcacg gtggaacact tactggtgat gccgataacg
     5341 ccgtttgaga tcatgatggc gaagatctgg tcgatggggc tggtggtgct ggtggtatcg
     5401 ggattatcgc tggtgctgat ggtgaaaggt gtactgggcg taccgattga aggctcgatc
     5461 ccgctgttta tgctgggcgt ggcgctcagt ctgtttgcca ccacgtcaat cggcattttt
     5521 atggggacga tagcgcgttc aatgccgcaa ctggggctgc tggtgattct ggtgctgctg
     5581 ccgctgcaaa tgctttccgg tggatccacg ccgcgcgaaa gtatgccgca gatggtgcag
     5641 gacattatgc tgaccatgcc gacgacacac tttgttagcc tcgcgcaggc cattctctac
     5701 cggggtgccg gattcgaaat cgtctggccg cagttcctga cgctgatggc aattggcggc
     5761 gcgtttttca ccattgcgct gctgcgattc aggaagacga ttgggacaat ggcgtaagga
     5821 agggaaactt gacttcatac tgtgatgtca tactattgat gtcataaagt gaagtcataa
     5881 ggacatgcca tgagaaccaa ggtacaggct ttacggaaga aacaaaaaat acattggatc
     5941 aaatatttaa aactcctgtt cctcaaggga tcaagtggtc tgatatagag tcactggtta
     6001 aagcattagg cggagaaatt aaggaaggaa gaggttcgcg ttgtaagttc atactaaata
     6061 tgagcgttgc gtgtttccat cggcctcatc cgtcgccaga taccgataaa ggcgctgtag
     6121 aaagcgtgcg tgactggtta ctaagtatag gggtaaaacc atgatcaaac tgaagacgcc
     6181 aaactctatg gagattgccg ggcaaccagc tgttattacc tacgtgccag aactgaatgc
     6241 atttcgtggc aaattcctgg gcttgtcagg ttattgtgat tttgtctctg acagcattca
     6301 ggggctgcaa aaagaagggg aactttccct gctagagtat cttgaggatt gtaaagctgc
     6361 gggtattgag ccgtatgcgc gtacagaaaa gattaagacc tttacgttac gctatccaga
     6421 atcgctgagt gaacgcctta ataatgcggc tgcgcagcaa caagtctctg ttaataccta
     6481 tattattgaa acactgaatg agcgtttgaa tcatttataa attttagcgg cgtacattac
     6541 gcagaatatc tgcctttaca ggatctatct atgcaactga acttaatttt ataaattaag
     6601 ttcagttaat atcttttctt tcctcgtttc agaaataact caatcttcct tcggcaagca
     6661 ctgcaaatgc ccatgccgca cgccgcgctg ggcgataacg tcatcggcaa aatgctgcac
     6721 gtcgcccata tcacctttca gcacggcgat ttccagacag tcgtcgtgat tgatatgcac
     6781 atgcagcgtg gcgacggaga gatcgtggtg atggtgctgg gtggagacaa tgcggctggc
     6841 taagtcgcgt ttttcgtgtt catacacata ggagagcacc gcgaaacctt gcgtgccgtg
     6901 ctgctgagtg gcctcctggg ccagggcgct acgcagaata tcgcggatag cttcggaacg
     6961 gttgttataa ccacgacgct ggctcaggct gtccagcgtc tccagtaaat cgtcatcaag
     7021 cgtgatggtg actcgttgca tttgcgttaa accttttctg tggcgcgacg gcgcacgggg
     7081 aatgcgggta ataccgcgtt ttgtagcaca cgtccggcgt cagaggaaaa ggttaattta
     7141 tctcccaccg cctgggtttc gacgatttgt ccgttgtcca tcaccatcac ccgctggcaa
     7201 aagcgttcta caagacgtaa gtcgtgggtg atgaacaggc aggcggtgcc aaactgttgt
     7261 tgcagctttt tcagcaggcg aatgacgccc gcctgtaaca cgagatcaag gttagaaacg
     7321 gtttcatcca gaatcagcaa tttcggttcg accgccagcg cgcgtgccag gcagacgcgc
     7381 tggagctggc cgccgcttaa ctgcggtggg cgtttgtcga gaacgctgtc atcgagatcg
     7441 accgccttca gcatttcgct ggcgcgcgcc agttgttcgg attttttcag tgatagcagg
     7501 tggcgcatcg gttcacgcag gatctcgcgc acggttttgc gcggatttac ggcactgatg
     7561 gagtcctgaa acaccatctg aatatcacgg cggaaggctt tacgttgggc gcgattaagt
     7621 ttcgccagcg attcgccgcg ccagctaata ttcccttgtg agggagactc taagcctacc
     7681 agcagccgcg cgagggtgct ttttccgcag ccgctgcgtc ccagcagggc gacggtttcg
     7741 ccgcttttca gggtcaggga aacgttattc agcaccgcct gatgttgatg ttttccgcta
     7801 aatccaccgt gggcatagtg atgggaaagg ccgctgacgt taagtaaggt catgatgcca
     7861 gctccatacc gtagagggcg agatgagcgg aaaccaggct gcgcgtcacc gtatgtttgg
     7921 gggcgttaaa cagcgtttct acatcgccct gttcgacaat tttaccttgt gacatcaccg
     7981 ccacgtcatc cgccagacgc gccacaacgc ccatatcatg ggtgaccagc agcattcccg
     8041 gcgcttgttt ttgcataatg ctttccagca gatcgaggat gcgcgcctgt gctaccacgt
     8101 cgaggtcggt ggtcggttca tcggcgatga taaacggtga ttcacacagc accgccatcg
     8161 caatcatcat gcgctgcaac atgccgccgc tcatctcgaa cgggtacagc ttcagcacgc
     8221 gcgcggcgtt ttccagcccc accgcttcta tggcagcggt aagcgtagcg tcatcggcgg
     8281 gtttccctaa cgccaggcag gtttcccgcg cgtgggtgtg catggtgtgt agcggattaa
     8341 aggcgctgcg cgggttctgc atgatggtgg caattttgat gccgcgcagg gcgcagggat
     8401 aaaccgcttt gccatcggct aaaatttccc ccgccgtctg gcgaacgcca gcgggcaaaa
     8461 tgcccagcgt cgcggcgcag gttaatgatt tcccgctgcc gctaccgccg actaacgcca
     8521 gcacgcgccc gcgttgcagg gttaacgata caccgtgtac cagcggctgc gcggcctgta
     8581 gcgcgatatt acgtagttca atctgttgcg gcattagtgt gcgtgctccg tcaccagatg
     8641 agggtccaga tgatcgcgca gtgcgtcacc caccaggtta aaggccatca cgctgataaa
     8701 cagcgccagc cccggccaga acatttgcag cggctgggtc cagatatact ggcgcgcgtc
     8761 gttaatcatc acgccccatt cggcggtcgg cgcggtcaca ccgaggccaa ggaaagacat
     8821 ccccgagacg tgcagcatca tatggccgat atccagcgtt gccagcacca gcagcgaagg
     8881 gatcaccgcg cctgccagat gatcgacaaa cactcgcaca tggcccgcac cggaaagccg
     8941 tgacgccagc acaaactcgc gttggcgtag tgaaatcacc aggctgcgca ccatgcgtgc
     9001 ataccacgcc cagtgcgaca gggcgatggc gataattacg ttggtcagcc cggtgccgag
     9061 cacgccaacc ataaagaacg acagaatcga ggtcgggaag gtcataaaca tatcggcgac
     9121 gcgcatggtg gcctgatcaa cgcgcccgcc aatcaacccg gcgctgccgc caataaccag
     9181 ccctaatgtc agcaccagca gcaggcaggc cattaccgaa ccgagcgaca cgcgggtcgc
     9241 tgccatcagc cgcgagaaaa tatcgcgacc taagtgatcg gtgcccagcc agtgctgcgc
     9301 atccggcgaa agcaggtgcg acggcaaatc aatcgcctgg gggtcatacg gcagccacca
     9361 ctggctggtg agcgcaatca gcgccagcag ggcgataatg atcagcgcca ggcgtaccga
     9421 ccagcgggaa gagaggaaaa agttcacgcg tgcgctcctt catgacgacg aatgcgcggg
     9481 tccagcgcgg cgttgagcaa atcgacaatc aaattacaga ccacaaaaac caccaccatc
     9541 atcagcgtaa agaactgaat caccggatag tcacggttaa aaatcgccga caccgcatag
     9601 cgcccgacgc caggccaggc aaagatgttt tcgataatca tcgtcccgcc aatcaattcg
     9661 ccaatgtgca tccccacggc ggtgatcatc ggcagcgagg cattgcgcag aatatggcgg
     9721 cgttcggtct gtttgtcatt caggccgcgt agccgcgccc aggtgacgtg acgctgaccg
     9781 gcgacgtcca gcatactggc gcgcagcagt cgcgcgttaa tcgccagcga cataaaggcg
     9841 atggaaaccg caggcaaaat gatgtgctgc cagccgccgt agcccatcgc gggtagccat
     9901 tgcagataca ccgaaaacgc catcactagc aaaaatgcca gccagaagtt gggcatcgac
     9961 acgccaagaa acgcgataaa gcgcacggcg aaatccggca aacgatcgcg gtggcgcgcc
    10021 gcccagatac cgagcggtac ggaagtgagc agaattaata ccagcgccgc acctgcaagt
    10081 tccagcgtgg cgggcaggaa gttcagcata tcgtccagca ccgggcgttg gctggcgaat
    10141 gagataccaa agtcaagatg cagcgccttc cacaaccagg tgccgtactg gacgtacagc
    10201 ggctgatcca gtccgagcat ggtgcgggta gaggccagca tctccggcgt cggcggcagg
    10261 ttagacagac gcaaataatc gagcgccggg tcgccggtac cgaggcgcag catcagaaaa
    10321 atgatcaccg aggcggcgag caccatcggg atcagcagca gaaagcggcg taatacgtaa
    10381 cgcaacatta aggtttcacc ggtttaatct gttcgaacgg aatttcggtg gcgatcggcg
    10441 cgtaggggat gttacccagc tccggttttg ataccaccat cattgagatg taactgatag
    10501 gcagataaac cgcctcgtca tgcagacggg tcagaatgtc gcgatacagc gcctgacgtt
    10561 gcgtttcgtc atgggtcgcc agcacttcgc cgatctcttt atcaatcagc ggtttgtcgg
    10621 ctaatccttg ctgtgcctgg aagtcagcgt gcgacggtac gcgcattgaa ctcaggaagg
    10681 cgtgtggatc gtatggcgcg ccccaggtgc ggtggaaaat catgccaaaa cgaccgtcgc
    10741 gctgacgagc atagatactg ctctcttctt cgccaatcag cgagacatct gcgccaatct
    10801 ggcgcatatc agcctgaatg atttccgcca tcgatttgct taacgcatcg gtgccgatga
    10861 acgacagttc aatgcgcagc ggctgaccat ttttctcgcg gatgtctttg cccgcaggca
    10921 gcgtccaacc ggctttttcc agcaacgctt tcgctttctg cggatcgtac tggcgcggtt
    10981 tcaggccgag gttggcgtag ggcacagaag gggcaaacag ggtgtcggcg acctgctggg
    11041 tgccatacaa cgcgttatca atcagcgatt ttttgtttac cgcgtaatta agagcttcac
    11101 gtactgccag ctcattggtg ggggttttgg cggtattaag cgccagcatc acggtttcga
    11161 tcggctgtga cagttgggta tggtaagccg ggttctggct aaagcgggcg aaggtatcga
    11221 gcggtaataa cccttcgttt ccgtacagca ggtcgatatc gccagtttca aacgccaccg
    11281 cgcgggtagt cgggtccggg atgacgttaa aggtgatctt tttaatcgct ggcttttcgc
    11341 cccagtagtt ttcgttacgg acgaagacat cgtactgatt cagtttcgat tcctgcaaaa
    11401 tccacggtcc ggtgccaatc ggcgctttaa ttccgttcat ggtttcatgg tttttaaact
    11461 gcgagggagc gataaaacgg aaaggacggg gcagggccag ttcttgcagg aaaggatagt
    11521 aggcgctttt cagggtaatt tgcagctctg ttttactgag tgctttaaca tcaacaatct
    11581 ggtttgccag ctccagccag gcgtgacgtt gacggttatc gagcactgcg cggaagtttt
    11641 ctgccgccgc ctcggcatcg aacggttcac cgttggataa tttcacgtca tcacgcaggg
    11701 tgaaggtcca ggttttacca tcttctgaat gagtccagct ttttgccagc cacgggatca
    11761 ccgaaccgtc tgcctgatat ttcaccaatg gttcataaac catgctctgg gcgaacatct
    11821 ggttaggcgt gtaaaggtgc gggtttagtg gcccgacatt caccggccag gcggtagtga
    11881 tttcatctgg tgcagcggca tggacgataa aagacgcaca agccagcagc gcaaatagag
    11941 tgcggcggag tgtggagagc atgataaccc caatggatta aaatagatgg cggaaataag
    12001 tatgacgatt ttaagtattc gtcatactga ttacctgttc tggatcaata gatgggcagt
    12061 cgggtgggcg gatcagttaa ctgaatcgat ccattgcaca ctgtcggcgg tgagcgtaaa
    12121 gggggtgggg gtgcagatcg caaggcttaa attttctagc tgacaatggc tgactgacag
    12181 cgaggagtga taggtgctat ctacgctgac gatttgccag gcgctgccgc cacgctgttt
    12241 aacgatggct tctttgcgcg tccagatgcg ccagaacatt tccagttgct gatcaggatg
    12301 cacggcgtcc atttcagcgt gttccccgag gctgaacacg gcgttcgcca gccagcgcca
    12361 gttggcgcgc gggcgaatca cttcgatatc gcagccgact tcgccttcat cgctcaacag
    12421 cagagcgata tcgtcaccgc tatggcttaa gttgaaccat agcggcgttt ccggcgcgaa
    12481 tgcaggtttg ccttgttcgc catagatgat ctccggtagc ggggaaagcg tgtgcgaaag
    12541 caatgcacgc cccgccagcc agcgttcgcg tcgtggacct tgcggtgctt gctcgcgtaa
    12601 acccggtggc agtggagctg cgcttaaggt cgaaactttc cccagaacta tccgatacat
    12661 atcagggcca acgtttaatg gaaaatgaaa gtgcgtatcg tatcacttgt cgcctcatcc
    12721 cggtaaccga cttttcggtc tgcctggccc cagtaaaatc gccagtttgc taccgccttt
    12781 ggtggtttcc atccagattt tacacacgct ggttaatggc accgaaagta gcatccctac
    12841 cgggccgagc agccatcccc aaattaacaa tgaaagaaat accaccatgg tggacatccc
    12901 caggcgatgg cccatcatcc gtggttctaa aatattgccg atgaccatat ggaccactaa
    12961 aaacaatgcg ccgaccagaa tacatgcgta aacaccatta aacagcagca cctgaatcat
    13021 tggcggtacg gcggaaatta ccgcgccgat attgggcacg tagttgagca aaaacgccag
    13081 tactgcccac atcagcgcaa actgtacgcc cattagcgcc agccccagcc agacgatgac
    13141 acctgtccat aaactgagta gcgtctttaa cgccagataa tgcgaaacgc ctttaagtgc
    13201 gcggtgtaat cctgcgatgt gaatctgtgg gttattcagc gcaaaacgca ttttgtaagg
    13261 gacgtggcgc acttcaaaca gcataaaaac tacggtcatc accagcaaaa gcacgctcgc
    13321 cattgccccg gaaagcccga tcattagcgc tgtggtgaag gtaaccactt tttccgagtc
    13381 catccgctgc agcattcgct ccggcgacat atgcaaatta agaaaaggca acatctcctg
    13441 caatttaaaa agtttgcgcg tcagctcctt attaaacttc ggcagcatag agataaattc
    13501 gttaaacgat gccgccagta cgccgactag cgcggttagt gcgatcagca tcaccaccac
    13561 tacaatcgta atggtaacgg ggcgttgtac tccccgacga ataaaccagg taacgagcgg
    13621 gttgaggacg atggcaaaaa acagcgccag taacagctgc acaatgatat ctgccgctgc
    13681 gtgaatgccc gcgaggatca ctaccagcga ggccaacttg agcagaatgt gcatgcccgt
    13741 tttatcgggt tgaggggttt ccattggggc ttccttgtga ctttttgtgt taagtgtagc
    13801 gggagtcacg tcagcaatat tctgattctt actgctgatt ttcacgtcag cacgtggtaa
    13861 aaatgaaaca ctgttgtaaa aatgtggtga tcctcatgcc cgaacccgta gccgaacccg
    13921 cgctaaacgg attgcgcctg aatttgcgca ttgtctccat tgtcatgttt aacttcgcca
    13981 gctacctcac catcgggttg ccgctcgctg tattaccggg ctatgtccat gatgtgatgg
    14041 gctttagcgc tttctgggca ggattggtta tcagcctgca atatttcgcc accttgctga
    14101 gccgtcctca tgccggacgt tacgccgatt tgctgggccc caaaaagatt gtcgtcttcg
    14161 gtttatgcgg ctgctttttg agcggtctgg gatatctgac ggcaggatta accgccagtc
    14221 tgcccgtcat cagcctgtta ttactttgcc tgggacgcgt gatccttggg attgggcaaa
    14281 gttttgccgg aacgggatcg accctgtggg gcgttggcgt ggttggctcg ctgcatatcg
    14341 ggcgagtgat ttcgtggaac ggcattgtca cttacggggc gatggcgatg ggtgcgccgt
    14401 taggcgtcgt gttttatcac tggggcggct tgcaggcgtt agcgttaatc attatgggcg
    14461 tggcgctggt ggccattttg ttggcgatcc cgcgtccgac ggtaaaagcc agtaaaggca
    14521 aaccgctgcc gtttcgcgcg gtgcttgggc gcgtctggct gtacggtatg gcactggcac
    14581 tggcttctgc cggatttggc gtcatcgcca cctttatcac gctgttttat gacgctaaaa
    14641 gttgggacgg tgcggctttc gcgctgacgc tgtttagctg tgcgtttgtc ggtacgcgtt
    14701 tgttattccc taacggcatt aaccgtatcg gcggcttaaa cgtagcgatg atttgcttta
    14761 gcgttgagat aatcggcctg ctactggttg gcgtggcgac tatgccgtgg atggcgaaaa
    14821 tcggcgtctt actggcgggg gcagggtttt cgctggtgtt cccggcatta ggtgtagtgg
    14881 cggtaaaagc ggttccgcag caaaatcagg gggcggcgct ggcaacttac accgtattta
    14941 tggatttatc gcttggcgtg accggaccac tggctgggct ggtgatgagc tgggcgggcg
    15001 taccggtgat ttatctggcg gcggcgggac tggtcgcaat cgcgttatta ctgacgtggc
    15061 gattaaaaaa acggcctccg gaacacgtcc ctgaggccgc ctcatcatct taaaacttac
    15121 tgaataacca gcgtattaat gatgttttct gcggtggtct gcgctttttg ctgatcgtca
    15181 gcgggcagcg taatttgcat ggtcagcagt tgattaccca cgttacccag aataacggaa
    15241 gagtacgccg tctggccttt cgcggagata atactgtcta actgctgcat tttgtgacct
    15301 ttcagctcaa tggctttatt ggttaccact tgcagctgcg gatcgcggct acgttgctga
    15361 tcttccagac gcttcgccag caccgccaga tcttctttcg gatcatcgcc catgatgacg
    15421 atgactgctt tctgcccggt ggcgtcggac cagacatgca tgttattggc ctgcgttccc
    15481 agcttaccgc tctggtcggt catatccgct ggcagcgaga aacttaactt gccatcaagc
    15541 aggttgacgg gattcccggt agcgttattt tccgcgaccg aaccctgcgc cgtagcgtta
    15601 gtgtctttat catcacaggc cgcaagcccc ataaccagca ggccaattcc gacatattta
    15661 accagattgc gcattgactt cttcctttcg ataaacggcc ataacggctc attcatccat
    15721 cttatcacaa ctctgataac gaacctttaa ctcgcctgca aagcgttgat ttcggattta
    15781 tctgccagtc ttttcaacag catattgagt aatacgccat acattggcag gaagaaaacg
    15841 atactgatta acactttgaa acagtaatcg accagcgcga tttccatcca gtgttcagcc
    15901 ataaaggcat ccgggctacg ccagaaggca atgaagaaaa aggccagcgt gtcgctgacg
    15961 ttaccgaaca gtgtggacgc tgtcggtgcc agccaccagc ggcgactctg acgcaggcgg
    16021 ttaaaaacgt gcacgtcgag gatctgtccc agcgcgtagg ccatgaaact ggcggtggcg
    16081 atacgggcga caaacaggtt gaagtgggcg agtgcgccga atccctgcca ggaacccata
    16141 tagaatagcg acgagatgac gtaggagatc agcaacgccg ggatcatcac cgcaaagatg
    16201 atacgacggg ccagcggtgc gccaaaaata cgcacggtca ggtcggtagc aaggaaaata
    16261 aacgggaagc taaacgcgcc ccaggtggta tggaaaccca aaatggagac gggaagctga
    16321 accagatagt tactggaggt gatcaccagc agatgaaata acgataacca gaacaacgcc
    16381 ttatagcgtt gagtttgcga gaaaacgttc atattgtacc tttttgatta accattgggg
    16441 tgagggaacc caatacgtac gacacgtctg ttaccttaac ataataacgt gcttaccctt
    16501 ttttcgagcc gccgcatgat actgctttac gttgacaatg caatggttaa ttttcacgca
    16561 atcgttaacc tggtttgctt acggactcac agggcgtaaa ctagcgccgt ttttttatgt
    16621 gatgagaaga aaatgaccga tctcttttcc agccctgacc acacactcga cgcacttggc
    16681 ctgcgctgcc cggaacccgt aatgatggtg cgcaaaactg tgcgcaatat gcagcccggc
    16741 gaaacgttgc tgattatcgc cgacgatccg gccactaccc gcgatattcc tgggttttgt
    16801 acctttatgg aacacgaact ggttgcgaaa gagacggatg gactgcctta tcgttatttg
    16861 attcgtaaag gcggttgatg ggggctgatt ggcttcgatg ccgccttttc ccctcaccct
    16921 aaccctctcc ccagaggggc gaggggaccg atcgcgctca atgttgcgat cggtttgcct
    16981 tatctcctgc gcaacaatct taacgcattc gctgtcacca gcaccgtcgc acccgtatct
    17041 gccagcaccg ccagccatag tccggtcatc cctaaaagcg tggtgacgag gaagatccct
    17101 ttcagtccca gcgcaatggt gatgttctgg cggatattgg cgtgagtggc gcgtgccagt
    17161 tcaatcattt gcaccaggcc gcgcaggtgg ttatgggtta atgctgcatc agcggtttcc
    17221 agcgccacgt ctgtgccact gcccattgca atcccaatgg cggcagcttt catcgctggc
    17281 gcgtcgttaa taccgtcacc gaccatcgcc agcggcgcat gttgattcag ctcggtcacc
    17341 gctttgactt tatattccgg caacaggccc gctttaaatt ccagccccag ctccccggca
    17401 attgccgccg ctgcgcgtgg attatcgccg gtgaggatca cccctttgac gcccagcgcg
    17461 ttcagttcac tgatggcagt tgcagcatcg gcgcgcaggg tatcctgcaa tgcaatgata
    17521 cccagcacgt catcattacg tactaccagc acaaccgttt gcccggcgct ttccagttca
    17581 ttaatcaaac cagcaaatgc atcagcggga tgtttcccgg cagcgcatat caacacgcgc
    17641 tcaccgttaa cctgcgcttc aatgccagac ccgaccagcg cccgctgtga ttcggcggcg
    17701 ggaatggcga gtgcagcaac ctgtgcttcg cgcacgatgg cctgcgccag tggatgcgtc
    17761 gcgctttgct cgaccgccgc cgccagtgtc agcagttcag attcactaat acccgttgcc
    17821 ggatgaatcg cggtaacgcg cggtttaccg acggtcagcg taccggtttt atcaaacgcc
    17881 acctgagtaa cacgacctag ttgttccagc gccgctccgc ctttaatcaa cgccccacga
    17941 cgcgctgctg ccgccagccc ggaggtaatc gccgcgggcg tggagataac taacgcacac
    18001 gggcagccaa tcagcagcag cgtcaggcct ttataaatcc actcctgcca gctggcggca
    18061 aacagcagcg gtggcaccag cgtcaccagc agggcgacgg ccataatcgc tggcgtataa
    18121 atacggctga aacggtcgat aaaccgctca atgggggcgc ggcgctcttc ggcttcttcg
    18181 atcagtttca gaatccggtc aatggcgctg gcacccggtt ctgacagcac ttccagcgtt
    18241 accagacggt ctacgctggt ggctcctgca ggaactttat cgcccgtcgc gcgctccacc
    18301 ggaatggatt ctccggtcag ggcgctttca tcaaaactgg caaacggtga gagcagttta
    18361 ccgtcggcag gcaaacgccc acctgcggcg acttcaatca catcgccagg ccgcaggctg
    18421 ttaatcgcca cctcttcccg ctcaccgtta cgcaggcgcg tggcggtttc tggtttcagc
    18481 gccattaacg cgctaacgcc ctgacgcgcg cggctggcgg cccagccttc cagtcgttca
    18541 ccaatcaaaa acagcagcaa caccatcgca gcttcagccg ttgcgccaat aaacagtgca
    18601 ccaatagcgg ctacgctcat taaggtttca atggcgaagt agctgccgga tttgatcagc
    18661 cgtaatgcct gatgagcaat cgggtacagc ccaaccagcg tggtcgcgat aaacgccagt
    18721 tgcccgaacg gatgattgaa ctgctccaga ccccagctga ttgccatcat cacgattagt
    18781 gtaatcagcg gcagattctc tttcaggcgc gatgcttgtg gctcgtcggc ggcctgctca
    18841 tcgcgcaggg aatagcccgc tttttgcacc gcagattcaa cttgtgcacg aatgtcattg
    18901 tcggcatcaa ccaccagttt ttcggtggcg aacaacacct gcacctgatt cacacctgca
    18961 agctggcgca cggcattttc taccttgcgc gcacaggcgg cgcagtccat gccactgact
    19021 ttccagctat agcgggtgcc ggagacgttt tcagagagcg ttggcgagct ggagcatgcg
    19081 ccgtcgcagc aacagtcgtt ggcgttctgt accgtggtta gcggtttgaa cgcagcaaat
    19141 tgaggggctt tcttgccgtg attgtcaggt gtcgacatgg catactccgg ttaagttttt
    19201 tctcattaac cgaaggatac actctggagt cgactccaga gtcaagtttt atcagaaata
    19261 cagtgagcgg acgatgagga agtgaccggc aaagtagcag gccgcagcaa tcgcgttatc
    19321 cgcacggaag cgtcggcgat agtggctccc cagccagaca aagttactga taaacagcaa
    19381 cgaagcgcca acaaacgcag agagcgccgg agcggtcgga cggaagaacc acagttcacc
    19441 tgccagccac accatcacca gcgtcatgcc gataaacgtg cagataggcc aacgatactc
    19501 ttccagccgc gtccagataa tcgccagtaa cagcgcaccc agaaccagca acaccagtgg
    19561 cagaggccag aagaaagaga gcgtcatctg actggcgaaa tagatggtgt acagcaggtg
    19621 cgagaggaaa aacgcgccga tggcgtacat cagacgttga cgtggcaaca gggttagcgc
    19681 atcgcccagc agtgaggcgc acagccctgc cagcaccaga tagctaatgg cgtcgaacat
    19741 cggcgcttgc caggccagta acagcaggag aagaagggtt aacggtttaa acacccagcg
    19801 ttgccaggtt ggcccacgat acgatgcatc cacagatagc catgcggaaa gacagacagc
    19861 gataaacgac caaagcatct tagttccttg aattcgttat tttttcgtaa gcggaatgcc
    19921 tacggtcttg cttccagttt agtggcgagg gtggacggat gacaacacca taactagcag
    19981 ggtatgctta tttccgcatt ttccgatgag ggataaagat gagcaaacca cctcttttct
    20041 ttattgttat cattggctta attgtcgtcg ccgcatcgtt tcgttttatg caacagcgac
    20101 gggaaaaagc tgataatgat atggctccgc tccagcaaaa gctggtggtg gtgagcaaca
    20161 agcgggaaaa accgattaac gatcgccgtt cgcgccagca ggaagtgact ccggcaggca
    20221 ccagtatgcg ctatgaggca agcttcaaac cgcaaagcgg aggaatggag cagacgtttc
    20281 gcctcgacgc ccagcagtac catgccctga cagtgggcga taaaggtacg ctgagctata
    20341 aaggaacgcg ctttgttagc tttgttggcg aacaataagg ttatttttta actttaaatt
    20401 tcttctgcca gaccagcagt tcaaacacgc cgaaaaggaa aatccgcacc ttttcggcgg
    20461 tggtcatctg cgggccatct ttcggtaaag tggatttcaa tagcgccagc tgcataccat
    20521 gcatcagcac ggtaaaaatc agcgccacgt taacgaaaat attcagcggg cgcgggaagg
    20581 gatgcaccag attgaggatt aaaaatcccc aaacgcagag cattaacaaa cgaccaatat
    20641 taatcagcat cactttctcc ttgtgcttcg cgttgataca gccgataagc cacctgaccc
    20701 gccacttttt cccgatgtaa tgaccagttt gccggaacag tgggcagacc gttttcgact
    20761 tcgctttcga cataaatcag ggcttcgtca gccagccagc cgttatcttc cagtaaattt
    20821 atcgtctctt ctaacaagcc acggcggaac ggtggatcga caaacacgat attatgcggt
    20881 gtaccttttt gcgccaggaa tgacatcgcg ttgctgttca ccacgcgtgc attgcctgct
    20941 tttagtgtcg ccagattctt aattaactgc tgagaaaccg cgcgatccat ctcaatcaac
    21001 gtggcccccg cagcgtagcg cgataacgct tccagtccca gcgcgccgct cccggcgaag
    21061 caatccagac attgggcgtc aacaatgacc ggagccagcc agttaaacaa tgtttcgcgt
    21121 acgcggtcgg tggtggggcg cagacctggg ctatcaggaa ccgggagttt acggcctcgc
    21181 cactgcccgc caataatgcg gatttggccg ctgccggaat gattcggttt tttcatgata
    21241 aattgctcaa tccgccagat aacacatact tgcaggcggt gatgtgaatt aagttaagtg
    21301 atagactatt tcatcatttt tttagctgct atgtacatag cgttaacgct gtgccatgaa
    21361 gcaacagcga ggagtgtagt cgcaaatggc gaaagaaaaa aaacgtggct ttttttcctg
    21421 gctgggcttt ggtcaaaaag agcagacccc ggaaaaagag acagaagttc agaatgaaca
    21481 accggttgta gaagaaatcg ttcaggcgca agagcctgcg aaggcttctg aacaagccgt
    21541 cgaagagcaa ccgcaggcgc atactgaagc cgaggcggaa acttttgctg ccgacgttgt
    21601 ggaagtcact gaacaggttg ctgaaagtga aaaagcgcag cctgaagcgg aagtcgttgc
    21661 acagccggaa ccggtcgtag aagaaacgcc ggagccagtg gctatcgaac gtgaagagct
    21721 gccgttgccg gaagacgtca acgccgaaga ggtttcgcca gaagagtggc aggctgaagc
    21781 ggaaaccgta gagattgtcg aagcggcgga agaagaagcg gctaaagaag aaatcaccga
    21841 cgaagagctg gaagcacagg cgctggctgc cgaagcggca gaagaggcgg tgatggtggt
    21901 tcctccggta gaagagcagc cggtggaaga aatcgctcag gagcaggaaa aaccgaccaa
    21961 agaaggtttc ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa atctcggttc
    22021 cggatttatc agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg aggagctgga
    22081 agaacagctg ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaatta tcaccaatct
    22141 gacggaaggc gcatcccgca agcagcttcg tgatgccgag gcgctctatg gcctgctgaa
    22201 agaagagatg ggcgagattc tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac
    22261 gccgttcgtg atcctgatgg tgggcgtcaa cggtgtgggt aaaactacga cgattggtaa
    22321 gctggcacgt cagtttgagc tgcagggtaa atcggtgatg ctggcggcgg gcgatacttt
    22381 ccgtgcagca gcggttgaac agcttcaggt ctggggtcag cgcaacaata ttccggtgat
    22441 tgcccagcat accggtgcgg attccgcctc tgttatcttc gacgccattc aggcagctaa
    22501 agcgcgtaat atcgacgtcc tgattgccga taccgccgga cgcctgcaga acaaatcgca
    22561 cctgatggaa gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg ttgaagcgcc
    22621 gcatgaagtt atgctgacta ttgatgccag taccgggcag aatgcggtaa gccaggccaa
    22681 actgttccac gaagccgttg gcttaaccgg catcacgcta acgaaactgg acggcacggc
    22741 gaaaggcggg gtaattttct cggtggctga ccagtttggt atccctatcc gctacattgg
    22801 tgtcggcgaa cgtattgagg atttgcgtcc gtttaaggcg gacgacttta tagaggcact
    22861 ttttgcccga gaggattaac aatgattcgc tttgaacatg tcagcaaggc ttatctcggt
    22921 gggagacagg cgctgcaggg cgttacgttc catatgcagc cgggtgagat ggcgtttctg
    22981 accggtcatt ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg
    23041 cccagcgccg ggaaaatctg gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa
    23101 gttccgtttc tgcgccgcca gattggcatg attttccagg atcaccatct actgatggac
    23161 cgtactgtct acgataacgt ggcgatcccg ctgattatcg ctggtgccag cggtgacgat
    23221 attcgtcgcc gggtgtcggc ggcactggat aaagtcgggc tactggacaa agcgaagaac
    23281 ttccctattc agctttccgg cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg
    23341 aataagcccg cggtactgct ggcggacgaa ccgaccggta acctggacga cgcgttgtcg
    23401 gaagggattc tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt attgatggca
    23461 acgcacgaca tcaacctgat ttcacggcgt tcctatcgca tgctcaccct gagcgatggt
    23521 cacttgcatg gaggcgtggg ccatgaataa gcgcgatgca atcaatcata ttcggcagtt
    23581 tggtgggcgt ctggatcgct tccgtaaatc ggtcggcggc tcaggcgacg gcggtcgtaa
    23641 cgcaccgaaa cgcgcgaaat cctcgccaaa accggtaaat cgcaaaacca acgttttcaa
    23701 cgaacaggtg cgctatgcct tccacggcgc attgcaggat ctgaaaagca aaccgttcgc
    23761 cacgttttta acggtgatgg ttatcgccat ctccctgacg ctgcccagcg tctgttatat
    23821 ggtgtacaaa aacgttaatc aggcggcgac gcagtattat ccgtcaccgc aaatcactgt
    23881 ttacttgcaa aaaacgctgg acgatgacgc cgctgcgggc gtggtggcac agttgcaggc
    23941 cgagcaaggc gtggagaaag tgaactatct ttctcgtgaa gacgcactgg gcgagttccg
    24001 taactggtct ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt
    24061 ggcggtggtg atcccgaaac tcgattttca ggggacggaa tcactcaata cgctgcgtga
    24121 tcgtatcacg cagattaacg gcattgacga agtgcggatg gatgacagct ggtttgcccg
    24181 tctggcggcg ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg tgttgatggt
    24241 ggcggcagtg ttcctcgtca tcggcaacag cgtacgtctg agcatctttg ctcgccgtga
    24301 ctccatcaac gtgcagaaac tgattggtgc gacggatggt ttcatccttc gcccgttcct
    24361 gtatggtggc gcactgctgg gattttctgg cgcattgttg tcattaattt tgtcagaaat
    24421 tctggtgctg cgattgtcat cggcggttgc ggaagtggca caggttttcg gaacgaattt
    24481 tgatatcaat ggtttatcgt tcgatgaatg cctgctattg ctgctggtat gctcgatgat
    24541 tggctgggtg gcagcgtggc ttgccacggt acaacattta cgccacttta cgcctgaata
    24601 ataaaagcgt gttatactct ttccctgcaa tgggttccgt agcagggaaa gagatcccgt
    24661 tgtctcttcc cggtatttca tctctatgtc acattttgtg cgtaatttat tcacaagctt
    24721 gcattgaact tgtggataaa atcacggtct gataaaacag tgaatgataa cctcgttgct
    24781 cttaagctct ggcacagttg ttgctaccac tgaagcgcca gaagatatcg attgagagga
    24841 tttgaatgac tgacaaaatg caaagtttag ctttagcccc agttggcaac ctggattcct
    24901 acatccgggc agctaacgcg tggccgatgt tgtcggctga cgaggagcgg gcgctggctg
    24961 aaaagctgca ttaccatggc gatctggaag cagctaaaac gctgatcctg tctcacctgc
    25021 ggtttgttgt tcatattgct cgtaattatg cgggctatgg cctgccacag gcggatttga
    25081 ttcaggaagg taacatcggc ctgatgaaag cagtgcgccg tttcaacccg gaagtgggtg
    25141 tgcgcctggt ctccttcgcc gttcactgga tcaaagcaga gatccacgaa tacgttctgc
    25201 gtaactggcg tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg ttcttcaacc
    25261 tgcgtaaaac taagcagcgt ctgggctggt ttaaccagga tgaagtcgaa atggtggccc
    25321 gtgaactggg cgtaaccagc aaagacgttc gtgagatgga atcacgtatg gcggcacagg
    25381 acatgacctt tgatctgtct tccgacgacg attccgacag ccagccgatg gcaccggtgc
    25441 tctatctgca ggataaatca tctaactttg ccgacggcat cgaagatgat aactgggaag
    25501 agcaggcggc aaaccgtctg accgacgcga tgcaaggtct ggacgaacgc agccaggaca
    25561 tcatccgcgc gcgctggctg gacgaagaca acaagtccac gttgcaggaa ctggctgacc
    25621 gttacggtgt ttccgctgaa cgtgtacgcc agctggaaaa gaacgcgatg aaaaaactgc
    25681 gcgctgctat agaagcgtaa tttccgctat taagcagaga accctggatg agagtccggg
    25741 gtttttgttt tttgggcctc tacaataatc aattccccct ccggcaaaac gtcaatcccc
    25801 acgcagattg ttaataaact gtcaaaatag ctattccaat atcataaaaa tggggtatgt
    25861 tttagcagag tatgctgcta aagcacgggt agtcatgcat aaaacgaaat aaagtgctaa
    25921 atcacaacac cacaacacac gtaataacca gaagaatggg gattctcagg atgaatataa
    25981 agggtaaagc gttactggca ggatgtatcg cgctggcatt cagcaatatg gctctggcag
    26041 aagatattaa agtcgcggtc gtgggcgcaa tgtccggtcc ggttgcgcag tacggtgacc
    26101 aggagtttac cggcgcagag caggcggttg cggatatcaa cgctaaaggc ggcattaaag
    26161 gcaacaaact gcaaatcgta aaatatgacg atgcctgtga cccgaaacag gcggttgcgg
    26221 tggcgaacaa agtcgttaac gacggcatta aatatgtgat tggtcacctc tgttcttcat
    26281 caacgcagcc tgcgtctgat atctacgaag acgaaggcat tttaatgatc accccagcgg
    26341 caaccgcgcc ggagctgacc gcccgtggct atcagctgat cctgcgcacc accggcctgg
    26401 actccgacca ggggccgacg gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta
    26461 ttgctatcgt tcacgacaaa cagcaatacg gcgaaggtct ggcgcgagcg gtgcaggacg
    26521 gcctgaagaa aggcaatgct aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag
    26581 atttctcaac gctggtggcg cgtctgaaaa aagagaatat cgacttcgtt tactacggcg
    26641 gttatcaccc ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc
    26701 agtttatggg gccggaaggt gtggctaacg tttcgctgtc taacattgcg ggcgaatcag
    26761 cggaagggct gctggtgacc aagccgaaga actacgatca ggttccggcg aacaaaccca
    26821 ttgttgacgc catcaaagcg aaaaaacagg acccaagcgg cgcattcgtt tggaccacct
    26881 acgccgcgct gcaatctttg caggcgggcc tgaatcagtc tgacgatccg gctgagatcg
    26941 ccaaatacct gaaagcgaac tccgtggata ccgtgatggg tccgctgacg tgggatgaga
    27001 aaggtgctct gaaaggcttt gagttcggcg tatttgactg gcacgccaac ggcacggcga
    27061 ccgatgcgaa gtaatcatta atcggcaact ttgggttgcc gccaaattgc taatatcgag
    27121 tacgttgctt catgccggat gcggcgtaaa cgccttatcc ggcctacaag atccaaagaa
    27181 atcagtaaat tgcaacacac attgtaggcc tgataagcgt agcgcatcag gcaatttctc
    27241 gttggtcatc acttttgaaa tcaacttgac gattaacgct tctcccagcc gccctgttgc
    27301 gccgtaaatc ccagtgcctg cataaacgcc gtcatcacac cgcgatcttc cacgcctgca
    27361 tccgccatcc accagcatga aacgccagga ttgttacgca aaacctcttc cagcagatat
    27421 tgccccacac cgcgacgtcg ggtgacttcc cgcacacgca gggaatccag tgctccctcg
    27481 gtgccgctta aggttacccg tacggcagcg agcaggcgct cgttaaaacg cgcggcgtag
    27541 atacggtggt tatcgtcaac ctgtaaagag gaaggggaat actccggcca gatcttttgc
    27601 aggtcaatcc ggtcctggtc gctaaagttt tctaatcgaa tgatggtcag cttcatgggt
    27661 aacccgtgta aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc ggaggctttc
    27721 tcttttttat ttcttttggc aggtgattaa ttttttaaca gcaataatta caaagttaaa
    27781 ac
//
LOCUS       DAJTOR010000037        27528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTOR010000037 DAJTOR010000000
VERSION     DAJTOR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.037"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(174..704)
                     /gene="ppa"
                     /locus_tag="OO249_001425"
     CDS             complement(174..704)
                     /gene="ppa"
                     /locus_tag="OO249_001425"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5635774.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            1014..1970
                     /gene="ytfQ"
                     /locus_tag="OO249_001426"
     CDS             1014..1970
                     /gene="ytfQ"
                     /locus_tag="OO249_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR5635775.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENRIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            2110..3612
                     /locus_tag="OO249_001427"
                     /pseudo
     CDS             2110..3612
                     /locus_tag="OO249_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            3626..4648
                     /locus_tag="OO249_001428"
     CDS             3626..4648
                     /locus_tag="OO249_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5635776.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            4635..5630
                     /gene="yjfF"
                     /locus_tag="OO249_001429"
     CDS             4635..5630
                     /gene="yjfF"
                     /locus_tag="OO249_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR5635777.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(5663..6661)
                     /gene="fbp"
                     /locus_tag="OO249_001430"
     CDS             complement(5663..6661)
                     /gene="fbp"
                     /locus_tag="OO249_001430"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5635778.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            6837..8210
                     /gene="mpl"
                     /locus_tag="OO249_001431"
     CDS             6837..8210
                     /gene="mpl"
                     /locus_tag="OO249_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5635779.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADTANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(8293..8844)
                     /locus_tag="OO249_001432"
     CDS             complement(8293..8844)
                     /locus_tag="OO249_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5635780.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            8938..10290
                     /gene="pmbA"
                     /locus_tag="OO249_001433"
     CDS             8938..10290
                     /gene="pmbA"
                     /locus_tag="OO249_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5635781.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGVDCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            10474..10860
                     /gene="cybC"
                     /locus_tag="OO249_001434"
     CDS             10474..10860
                     /gene="cybC"
                     /locus_tag="OO249_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR5635782.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(10905..11369)
                     /gene="nrdG"
                     /locus_tag="OO249_001435"
     CDS             complement(10905..11369)
                     /gene="nrdG"
                     /locus_tag="OO249_001435"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5635783.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(11468..13606)
                     /gene="nrdD"
                     /locus_tag="OO249_001436"
     CDS             complement(11468..13606)
                     /gene="nrdD"
                     /locus_tag="OO249_001436"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5635784.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYSNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(14000..15655)
                     /gene="treC"
                     /locus_tag="OO249_001437"
     CDS             complement(14000..15655)
                     /gene="treC"
                     /locus_tag="OO249_001437"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5635785.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(15705..17126)
                     /gene="treB"
                     /locus_tag="OO249_001438"
     CDS             complement(15705..17126)
                     /gene="treB"
                     /locus_tag="OO249_001438"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5635786.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNARQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(17227..18192)
                     /gene="treR"
                     /locus_tag="OO249_001439"
     CDS             complement(17227..18192)
                     /gene="treR"
                     /locus_tag="OO249_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5635787.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            18377..18430
                     /gene="mgtL"
                     /locus_tag="OO249_001440"
     CDS             18377..18430
                     /gene="mgtL"
                     /locus_tag="OO249_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5635788.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            18571..21267
                     /gene="mgtA"
                     /locus_tag="OO249_001441"
     CDS             18571..21267
                     /gene="mgtA"
                     /locus_tag="OO249_001441"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5635789.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(21473..21859)
                     /gene="ridA"
                     /locus_tag="OO249_001442"
     CDS             complement(21473..21859)
                     /gene="ridA"
                     /locus_tag="OO249_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5635790.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYETFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(21932..22393)
                     /gene="pyrI"
                     /locus_tag="OO249_001443"
     CDS             complement(21932..22393)
                     /gene="pyrI"
                     /locus_tag="OO249_001443"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5635791.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(22406..23341)
                     /gene="pyrB"
                     /locus_tag="OO249_001444"
     CDS             complement(22406..23341)
                     /gene="pyrB"
                     /locus_tag="OO249_001444"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5635792.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(23345..23479)
                     /gene="pyrL"
                     /locus_tag="OO249_001445"
     CDS             complement(23345..23479)
                     /gene="pyrL"
                     /locus_tag="OO249_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5635793.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(23759..24154)
                     /locus_tag="OO249_001446"
     CDS             complement(23759..24154)
                     /locus_tag="OO249_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5635794.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     SYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(24285..24998)
                     /gene="bdcA"
                     /locus_tag="OO249_001447"
     CDS             complement(24285..24998)
                     /gene="bdcA"
                     /locus_tag="OO249_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5635795.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            25069..25662
                     /locus_tag="OO249_001448"
     CDS             25069..25662
                     /locus_tag="OO249_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5635796.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            25807..26259
                     /gene="tabA"
                     /locus_tag="OO249_001449"
     CDS             25807..26259
                     /gene="tabA"
                     /locus_tag="OO249_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5635797.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            26382..27528
                     /locus_tag="OO249_001450"
                     /pseudo
     CDS             26382..27528
                     /locus_tag="OO249_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         6604 a         6814 c         7279 g         6831 t
ORIGIN      
        1 tccagcaagt ggctatgaac agcaaaatgg tggtcgggtt tgcttgttct taccgttcaa
       61 gccttgcaac cgctgagaat aatgatggtg gaatagagag tttatttatc gcgggcataa
      121 aaaaaccctt actaaccgaa gcccggcgtt cagggttatt gcgctagaag aacttattta
      181 ttctttgcgc gctcgaagga agcaacgatt tcagctttag cggcttctgc gttttcccaa
      241 ccttcaactt tcacccactt gcctttttcg aggtctttgt agtgctcgaa gaagtgagcg
      301 atttgcgctt tcagcagttc aggcagatcg tttacgtctt taatgtgatc gtattctttg
      361 ctcagcttgc tgtgcggaac cgcaaccagt ttcgcatctt caccggcttc gtcggtcatt
      421 ttcagaacgc caaccggacg gcaacggatc acagaaccag gctgcagcgg gtacggagtc
      481 gggaccagta cgtcaaccgg gtcaccgtcc agagacaggg tgtggttgat gtaaccgtag
      541 ttgcacggat agaacatcgc ggtggacatg aagcggtcaa cgaacagtgc gccgctctct
      601 ttgtcgattt cgtatttgat cggatctgcg ttagccggga tctcaataac aacgtagatg
      661 tcttccggca gatctttacc cgcagggacg ttgagtaagc tcatgtctgt ttcctttaaa
      721 aatatgtggc aaacaagtgc cgagtattat agccaactcg cgccgaatgt ctttgcttgt
      781 tttcgtcttc attttccttt ttcgccagtt ttaagacggt atacagagca ggaaaatcca
      841 taaccgtagc cgcatttttc atagtgagat gaaagcgatt acaaacttgt gattaacgtt
      901 ttatttactt ttttgaagtg tgatgtaacg caatccgtta cataacgaat tgtctatagt
      961 tttttcgcga acatcttttc accaataata attaccccga cgaggacaac cctatgtgga
     1021 aacgcttact tgtagtctct gcagtctcgg cagccatgtc gtctatggcg ttggccgctc
     1081 cattaaccgt aggattttcg caggtcggat cggaatccgg ctggcgcgcc gcagaaacca
     1141 atgtggcgaa aagtgaggcc gaaaaacgcg gaattacgtt gaaaattgcc gatggtcagc
     1201 aaaagcagga aaaccggatt aaagcggtac gttccttcgt tgcacaaggg gtggatgcga
     1261 tctttatcgc tccagtggtt gcgacgggtt gggaaccggt attaaaagag gcgaaagatg
     1321 ccgaaatccc ggtcttcttg ctcgaccgtt ccatcgatgt gaaagacaaa tctctctata
     1381 tgaccaccgt tactgccgac aacatcctcg aaggtaagtt gatcggtgac tggctggtaa
     1441 aagaagtgaa tggcaaacca tgcaacgtgg tggagctgca gggcaccgtt ggggccagcg
     1501 tcgccattga ccgtaagaaa ggctttgccg aagccattaa gaatgcgcca aatatcaaaa
     1561 ttatccgctc gcagtcaggt gacttcaccc gcagtaaagg caaagaagtc atggagagct
     1621 ttatcaaagc ggaaaacaac ggcaaaaaca tctgcatggt ttacgcccat aacgacgaca
     1681 tggtgattgg tgcaattcag gcaattaaag aagcgggcct gaaaccgggc aaagatattc
     1741 tgacaggttc tatcgatggc gtaccggata tctacaaagc gatgattgat ggcgaagcga
     1801 acgccagtgt tgaactgacg ccgaatatgg cgggacccgc ttttgacgcg ctggagaaat
     1861 acaaaaaaga cggcaccatg cctgaaaagc tgacgttaac caaatccacc ctttacctgc
     1921 ctgataccgc aaaagaagag ttagagaaga agaaaaatat ggggtattga gggttgctat
     1981 gcctgatgcc gattcgtagg ccggataagg cgctcgcgcc gcatccggcg atggtgcact
     2041 gaagcctgat gcgacgctta ccgcgtctta tcatgcctac tgggagcacg ctttacaccg
     2101 ggggaaacca tgacgaccga ccaacaccag gaaatcctcc gcaccgaagg attaagtaaa
     2161 tttttccccg gcgtcaaagc gttagacaac gttgatttca gcctgcgccg tggcgaaatc
     2221 atggcgctgc tcggtgaaaa cggggcggga aaatcaacgc taatcaaagc attaactggt
     2281 gtataccacg ccgatcgcgg caccatctgg ctggaaggcc aggttatctc accgaaaaat
     2341 accgcccacg cgcaacaact cggcattggc accgtctatc aggaagtcaa cctgctaccc
     2401 aatatgtcgg tcgctgataa tctatttata ggccgcgaac ccaaacgctt cggccttcta
     2461 cgccgcaaag agatggaaaa gcgcgccacc gaactgatgg catcttacgg tttctccctc
     2521 gacgtgcgcg aaccgctcaa ccgcttttca gtcgcgatgc agcaaatcgt cgccatttgc
     2581 cgggctatcg atctctccgc caaagtgctg atcctcgatg aacccaccgc cagcctcgac
     2641 acctaggaag tggagctatt atttggcctg atgcgtcagt tgcgcgatcg cggcgtcagc
     2701 ctgatctttg tcactcactt tctcgatcag agctatcagg tcagcgatcg gatcaccgtt
     2761 ttacgcaacg gcagtttcgt aggctgtcgg gaaacgcgcg agctaccgca gatcgaactg
     2821 gtaaaaatga tgctggggcg cgagctggat acccacgcgc tacagcgtgc cgggcgaaca
     2881 ttgttgagcg acaaacccgt tgccgcgttc aaaaattacg gcaaaaaagg aacgatagca
     2941 ccgtttgatc tcgaagtgcg ccccggcgag atcgtcggcc tggcaggctt gctgggatcc
     3001 ggacgtaccg aaaccgccga agtgatcttc ggtatcaaac ctgctgacag cggcacggcg
     3061 ttgatcaaag gcaaaccgca aaacctgcga tcgccacatc aggcttcggt actgggcatc
     3121 ggcttctgcc cggaagacag gaaaaccgat ggcatcatcg ctgccgcctc ggtgcgggaa
     3181 aatatcatcc tcgctctcca ggcccagcgc ggctggctac gacccatttc ccgcaaagag
     3241 cagcaagaga ttgccgaacg ctttatccgc cagcttggca ttcgcacacc ttcaactgaa
     3301 caaccgattg aatttctctc cggcggcaat cagcaaaaag tgttgctttc acgttggcta
     3361 ctgacccgac cgcaatttct gatcctcgat gagccaaccc gtggcattga tgttggtgcc
     3421 cacgccgaga tcatccgcct gattgaaacg ctatgcgccg atggtctggc gctgctggtg
     3481 atctcctccg aactggaaga actggtgggc tatgccgacc gggtgatcat catgcgcgat
     3541 cgcaaacagg tggcggagat ccggctggca gagctttccg ttccggctat catgaacgcc
     3601 attgcggcgt aaggagaaca gtgtgatgcc tcaatctctt ccggacacca ctacgccaaa
     3661 aaggcgcttt cgctggccga cggggatgcc gcagctggta gcactattgc tggtgctgct
     3721 ggtcgacagc ctggtagccc cgcatttctg gcaggtagtg cttcaggatg ggcgtttgtt
     3781 cggtagcccc atagacattc ttaaccgtgc agcccccgtt gcgttactgg cgattggtat
     3841 gacgctggtg atcgccaccg gtgggattga tctctccgta ggggcggtga tggctatcgc
     3901 cggagccaca acggctgcga tgacggtcgc ggaagtcagc ctgccgattg ttttgttaag
     3961 cgccctaggc accggcatcc tggcgggatt gtggaacggc atactggtag cgatcctcaa
     4021 aattcagcca tttgtcgcca ccctgatcct gatggtcgcc gggcgcggcg tggcgcaact
     4081 gatcacctcc ggacagatcg tcacgtttaa ctcgccggat ctctcatggt ttggcagtgg
     4141 atcgctgttg ttcctgccaa cgccagtcat tattgcggta ctgacgcttc tcctgttctg
     4201 gctgttgacc cgcaaaacgg cactggggat gtttatcgaa gccgttggta tcaacattcg
     4261 ggcggcaaaa aatgccgggg taaacacgcg gatcatcgtc atgctcactt atgtgttgag
     4321 cgggctgtgt gcggcgattg cgggcattat cgtggcggcg gatattcgcg gtgccgatgc
     4381 caacaacgcc ggattatggc tggagctgga cgccattctc gcggtggtga ttggcggcgg
     4441 atcgctgatg ggcgggcgct ttaacctgct gctctcggta gtgggggcgc tgattattca
     4501 ggggatgaac accggaattt tgctttcggg ctttccaccg gagatgaacc aggtggtgaa
     4561 agcggtggtg gtgctttgcg tgctgattgt ccagtcgcaa cgctttatca gtctgattaa
     4621 aggagtacgt agccgtgata aaacgtaatt tgccactgat gatcaccatc ggcgtctttg
     4681 tgctgggcta tttctactgc ctgacgcagt ttcccggttt tgcttccaca agagtgatct
     4741 gcaatatcct gaccgataac gcttttctgg ggattattgc cgttggtatg acctttgtga
     4801 tcctctctgg cggtatcgat ctctccgtcg gttcggtgat cgcctttact ggcgtgtttc
     4861 tggcaaaagt ggttggcgat ttcggcctct cgccgctgct ggcgtttccg ctagtgctgg
     4921 tgatgggctg tgccttcggc gcatttatgg ggcttctgat cgacgccctg aagatcccgg
     4981 catttatcat tacgcttgcg gggatgttct ttttgcgcgg cgtcagctat ctcgtttcgg
     5041 aagagtcgat tccgataaac catcccattt atgacacgct ctcaagcctt gcgtggaaaa
     5101 tccccggcgg cggtcgcctt agtgcgatgg ggctgttgat gctggcggtg gtggttatcg
     5161 gtattttcct tgcgcatcgt acccgttttg gcaatcaggt gtacgctatt ggcggcaacg
     5221 cgacgtcggc gaacctgatg gggatttcta ctcgcagcac tactattcgc atttatatgc
     5281 tctccaccgg actggcaaca ctggcgggga ttgtcttctc gatttatacc caggccggat
     5341 atgcactggc gggcgtaggt gtggaactgg acgccatcgc gtcggtggtg attggcggta
     5401 cgttgttaag tggtggcgtc gggacggtgc ttgggacgct ttttggtgtg gcaattcagg
     5461 gactgatcca gacttacatc aactttgacg gcacgctgag ttcctggtgg acgaaaatcg
     5521 ccatcggtat tttgttgttt atttttatag cattacagcg tgggttaacg gtgctgtggg
     5581 agaatcgtca gagttcgcca gtgacaagag tcaacattgc gcagcgataa aaatgcctct
     5641 ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag cgttcgacat
     5701 cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcaga gtttccggga
     5761 tgatatccag aatacgctct ttgccatcgc tcgctttacc gcccgcttgt tccgccagga
     5821 atgccatcgg gttgcactca taaagcaaac gcagtttgcc gtccgggtgg ctggcggtgc
     5881 ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca accagtgaac
     5941 cgatataacg tgaggtatac gggcggttgg tggatttatc ttcttcctgg cagaatttaa
     6001 tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg gagtaggttt
     6061 tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc agcgaaggat
     6121 cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatggta gaggagccgt
     6181 ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct tcttccgtta
     6241 ccggcgtgcc gacgggcgta actcggcggt agatggagaa aatggtaccg acagagacgt
     6301 taacatcgat gttggacgag ccatccagtg ggtccatcag caccacgtat tttgcatgtt
     6361 cacagccttc aaagacaaca atctcatctt cttcttcaga ggcaatgcct gcaacgatat
     6421 cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt ttttgctgaa
     6481 cctcgccctg cacgttctca gcaccgctgg cacccaggat atcaaccagt cctgctttgt
     6541 tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa gcagtgagct
     6601 caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct aacgttttca
     6661 taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta acaaataatc
     6721 tcaatgttgc gctcaggtga atcgcgccag caaattacgg attatcctga aatgcgtttc
     6781 tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag tgacgtatgc
     6841 gcattcatat tttaggaatt tgtggcacgt ttatgggcgg tctggcgatg ctggcgcgcc
     6901 agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg agcaccttac
     6961 ttgagaagca aggcattgaa ctgattcagg gttacgatgc cagccagctc gatccgcagc
     7021 cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg gaagcggtac
     7081 tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt gtgctgcgcg
     7141 accgctgggt gctggccgtt gccggtacac acggcaaaac caccaccgcg ggaatggcga
     7201 cctggattct ggaacagtgt ggttacaaac cgggatttgt gatcggcggt gtgccgggga
     7261 actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa gcggatgagt
     7321 acgactgcgc cttcttcgac aaacgctcta aatttgtcca ttactgcccg cgtacgctga
     7381 tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa gcgatccaga
     7441 aacagttcca ccatctggtg cgtatcgttc cggggcaggg ccgtattatc tggccggaaa
     7501 atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag gagctggtgg
     7561 gtgagcaggg gcactggcag gcgaaaaagc tgaccactga tgcttccgaa tgggaagtct
     7621 tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct ggtaggcgaa cataatatgc
     7681 ataatggcct gatggcgatt gcggcggccc gccatgttgg cgtagcgccg gcagataccg
     7741 ctaacgcgct gggttcgttt attaatgctc gtcgccgtct ggagttgcgt ggtgaagcga
     7801 atggcgtcac ggtatatgac gattttgccc atcacccgac ggcgattctg gcaacgctgg
     7861 cggcgctgcg tggcaaagtt ggtggtacag cgcgcattat tgctgtgctg gaaccgcgct
     7921 cgaataccat gaaaatgggg atctgcaaag acgatttggc accttcatta ggtcgtgccg
     7981 atgaagtctt cctgctgcaa ccggcgcata ttccgtggca ggtggcagaa gtggcagaag
     8041 cctgcgttca gcctgcacac tggagtggcg atgtggatac gctggcagat atggtggtga
     8101 aaaccgctca gcctggcgac catattctgg tgatgagcaa cggcggtttt ggtgggatcc
     8161 atcagaaact gctggatggt ctggcgaaga aggcggaagc tgcgcagtaa ttcggcctca
     8221 gcctgagata gcattgccgg ataaggcgtt tacgccgcat ccggcaaacc atactcacat
     8281 caacaacgaa aattactctt cattctccgc cagttcgcgc agatactgga aaatctggcg
     8341 tgcggatttc ggcggcttat tcccttcttt ctctttcttc gcgttgcgga tcagagtacg
     8401 cagttgctga cgatcggcat cgggccacag atttaatacc tcagcgatgg cgtcatcacc
     8461 ttgatcgatc agacgatcgc gcaggttttc cagtttatgg aacagcacca cctgctggtt
     8521 gtgacggttt ttcagcttat ccagcgcctg acggatcggt tctacatcgc gctggcgcag
     8581 catcttaccg atgagctgca actggcggcg gcgaccttcc atcttaatac gctgggcgag
     8641 ctcaatagcc gcgcgcagat ccgcatctaa cgggatctta tccagcgcgt ttttccccag
     8701 atcaacaatt tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa tttcactttt
     8761 actgacccag ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa cgtcgtcgag
     8821 ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa aaagaggcta atgttaccag
     8881 ttaagatgcg cactgaaaaa cggttctctg ttagacttca gagaaactct ctacattatg
     8941 gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct ggaagaagca
     9001 gtttccactg cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt tgccgtcagc
     9061 aagaccaccg gcattagcgt aagcacgcgt tatggtgaag tggagaatgt tgaattcaat
     9121 agcgatggcg cgctggggat cactgtttat caccagaacc gcaaaggtag cgcatcatcc
     9181 accgatttaa gcccgcaggc cattgcccgt actgtacagg cggcgctgga tattgcccgt
     9241 tatacctcgc cagatccctg tgccggcgtg gcagacaaag agctgctggc ctttgacgca
     9301 ccagatctcg acttgttcca ccctgcggaa gtttccccgg atgaagccat tgaactggcg
     9361 gcccgcgcag aacaggcggc attgcaggcg gacaaacgca tcaccaatac cgaaggtggc
     9421 agctttaaca gccactacgg tgtcaaagtt tttggcaaca gccacggcat gttgcagggt
     9481 tactgctcaa cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa tggcgatatg
     9541 gagcgtgatt acgcctacac cattggtcgt gcgatgagcg atctgcaaac gccagagtgg
     9601 gttggggtcg actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa actctccacc
     9661 atgaaagcgc cggtcatttt tgccaatgaa gtggcaaccg ggctttttgg ccatctggtg
     9721 ggggcgatag cgggtggatc ggtttaccgt aaatctacct tcctgctgga ttcgctgggt
     9781 aaacaaattc tgccggactg gctgaccatt gaagagcatc cgcatctgct gaaagggctg
     9841 gcgtcgacgc cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat tattaaagat
     9901 ggcatcctga ctcagtggct gctgaccagc tattcggcgc gaaaactggg gctgaaaagc
     9961 accggacatg cgggcggtat tcacaactgg cggattgccg gacaaggtct aagcttcgag
    10021 cagatgctca aagagatggg caccgggctg gtggtgacgg aattgatggg ccagggcgtg
    10081 agtgcaatta ccggtgatta ttcccgtggt gcggcgggct tctgggtaga aaacggcgaa
    10141 attcagtatc cggttagcga aatcaccatc gcaggtaatt taaaagatat gtggcgcaat
    10201 attgtcaccg tcggtaacga tattgaaaca cgcagtaata tacagtgtgg ttctgtgctg
    10261 ttgccggaga tgaaaatcgc cggacagtaa aaaaactggc gcgacctgtc gcgctataaa
    10321 ctaaaaaatt acacaaaatc attcgcactg catcgagacg gaaagtgaat gagggccggg
    10381 gagcgtactc gcagtacgtg acccggtcga atgagcgtag tcgtcgatga ggcagtgtga
    10441 atgatgaagt gtaaataaaa aaggaagtga gcaatgcgaa aaagcctgtt agctattctt
    10501 gcagtctcct cgttggtatt cagttcggcg tcgtttgccg ctgatcttga agacaatatg
    10561 gaaaccctca acgacaattt aaaagtggtc gaaaaagccg ataacgcggc gcaagtcaaa
    10621 gacgcgttaa cgaagatgcg cgccgcagcg ctggatgcgc aaaaagcaac gccgccgaag
    10681 ctcgaaggca aatcaccgga cagcccggag atgaaagact tccgtcacgg tttcgatatt
    10741 ctggtcggtc agattgacga cgcgctgaag ctggcaaatg aaggtaaagt aaaagaagcg
    10801 caggctgccg cagagcaact gaaaacgacc cgcaacgcct atcaccagaa gtatcgttaa
    10861 ttcctcattt ccctgttgcc tgcactcagg taacagggaa cttatcatcg caaatgatgc
    10921 accacctgat tgctgctgcc gcgccagatc agggaagggt cttttaaatc ctgcacaaat
    10981 ttgccgtcga ccagcacgtt aatcaaatca acaacctgca tttgcgctgc gttgagttcg
    11041 tcgagtttat agcctgtcca cacccagatg tctttacccg gacactcggc gcggatgcgt
    11101 tgtaccagtt tcagaatatc cggcacgttt tgcggatgca gcggatcgcc gccggagagg
    11161 gaaataccct ggcgtttgat acgagtgtca ttcagatcgt taatgatctg gtcttccatt
    11221 gctttagtaa atggctgacc ggaatttacc cgccatgtgc ttttgttata gcaaccgggg
    11281 cattcatgaa cacacccgga gacaaacaga gtgcaacgag tgccggggcc gttgacgatg
    11341 tcgacaggat agtactgatg ataattcatt atttcgcctg gcttatcagt ccgcgtgtag
    11401 actgcatccg gtaatcaatg cctgatgcga cgctttcaca tcacatccgg catttgctta
    11461 gtgcagatca acctatctgc ccatttccca aatgtttaac gcggcgctta acttcttcct
    11521 gcttgccggc gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag
    11581 ttaccgacac acgggaggcg tcatggttac cacatttcgg gcaggtgaag cctttgctgg
    11641 tgcactcgaa ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc
    11701 cgtaatacgg tacatgctga tagctgtaat cccagacatc ttccagcgcc ttcaggttgt
    11761 gctgaatgtt tgaatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag
    11821 gcgcttcaaa gtcgatcttg tcgtacgggt tcaccttctt ctccacatcg aggtggaaac
    11881 tgttggtgta gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga
    11941 gacggcagaa gcggtcgcac aggttttcac tcggcgtgct gtagagactg aaaccataac
    12001 ccgtttcttc cttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt
    12061 tcgcgcgaag ctgctcgttg tcgtaaacat gctcgccgcc gaacagcgcg ttaatggttt
    12121 cgtggatgcc gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa
    12181 catcatcgtc agcattgaga cgcacgccac aagcaccttc catatagagg atcggggcca
    12241 cgcgcgcttt cacgccttcg agacgagcga tacgggtcat cagcgcctta cgtgccagca
    12301 ccagacgttc atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa
    12361 tacgcggcag gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc
    12421 cgttttcatt ttcccacacg ccgaggaagc tgcggcagcc catcggagtt ttaaacgaac
    12481 cggtgacttt cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc
    12541 actccagcgc cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat
    12601 cgcgaatcgc aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg
    12661 cgatacggtt acgcaggata gattcctgaa tcaggcgcga ctcccagctg gtgcccagac
    12721 caaaaccaaa ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt
    12781 actccagcga ctggaaggca tcgtagcact ctttaatggt acgagagtta gcgtagcctt
    12841 cggcgtccgg gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtga
    12901 caaacggtgc cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg
    12961 cgacctgagc aataatctgc gcagttacag cggttgccgt agagatcgac ttcggcggtt
    13021 caatctcggc gttccccatc ttaaagccct gggtcagcat gcctttcagg tcgatcaaca
    13081 tgcagttgaa catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac
    13141 gctcatgtgc ctgcaccacg tcacgcggca gcaggtgctg acgtgcatag tgtttagcca
    13201 cgatcccggc cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt
    13261 cgttgagtaa cgaggcgttg gtctgctcga ccagaccacg gatctcctga ttcaggcgac
    13321 cgcgtttttc acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt
    13381 gtttgtatgg acctgacatc agctggtttt caacagcggt ctggatctca ttgatatcca
    13441 cctggttgcg gccctgcatc tgctcgctga caactgcagc aacagtggcg caataatcgg
    13501 catcatcgac ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt
    13561 taaacggcac tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt
    13621 tttaagaaca ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt
    13681 ttccggtgct ttccgcaatc gccatccgct tttccacaac gccgcacctt tttcgtgggg
    13741 agctgtagta gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag
    13801 tctatatata gtgctttgca tcaaggatgt ttgaactttt tttgatgtag ctcaaagtaa
    13861 aaagcagagc gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc
    13921 tggttgaaaa ataaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt
    13981 accggggctt agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat
    14041 tcatggcaca gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc
    14101 cgcgcatttg ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca
    14161 aggtttgccc cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat
    14221 cctggtaatt gccccatgtc aggatggctt cctgcttacg cagtgcgatt aacttttggt
    14281 aggtgtaaaa caccgaggaa tcatcggcca gcgcggcttc tacgttgatt tgttgatagt
    14341 tatcacccag gccaatccac ggttcgccag ccgtaaatcc ggcattatcg ccgttgctcc
    14401 attgcatggg cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt
    14461 cggcatcacg accatcgttg cgcagctcgg caaacatatt gaggctctct acgtcgcgat
    14521 agtcagtaat gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat
    14581 acggcgttcc ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt
    14641 attcaccttc atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca
    14701 aggcattcca tgctacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg
    14761 ccacaaagtc aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt
    14821 gatgaaaatt aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat
    14881 gctcaaggct ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat
    14941 cgcggttcat ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc
    15001 cgtcgccatc caggtcatca gggaaacgcg ggtctttgga gatcagattc accacatcca
    15061 ggcgcaaccc gtcgacccca cgatcggccc agaactcaca gacttttttc agcactgcgc
    15121 gtaccgctgg attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt
    15181 actgttcgct ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt
    15241 tcggtggcgt ttctggttct ccatcgcgcc agatataaaa ctggcggtaa gggctttctt
    15301 tgttcagcgc ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga
    15361 gaatgatacg aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg
    15421 tgccgtaggt gggatcaatc gccgtatagt tcgctacgtc gtaaccgtta tcgacctggg
    15481 gagagacata aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca
    15541 ggcgttggat aacgccacgt aaatcgccgg taccgctacc cgtggtgtcc tgaaaactct
    15601 ttggataaat ctggtagata acgccgtttt gccaccagtg gggaggatta gtcattacgt
    15661 tattcctgca aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc
    15721 gtgcccaggc ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg
    15781 gcgatagcca ttgccagcgc aaacacctgc cagtagttcg gttgaatcga gagaattccc
    15841 ggcaggccgc ctacgccgat gccattcgcc ataacgccgt tcaggccgca tagcaatcct
    15901 gccagaccag aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg
    15961 tacattgcag gctcagtgac cccaagccag gcggagatcg ccgcaggcac ggagatctcg
    16021 cgttcattgt gcttgcggct ggaaatgata atgccgatca cggcggagcc ctgagcgata
    16081 ttcgacagcg caatcagtgg ccacactggc gtaccaccca tgctttgaat catctgcaaa
    16141 tcaatagcaa gcgtggtctg gtgtaccccg gtgatcacca gcggggcgta caggaagcca
    16201 aacaatgccg cgccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca
    16261 acgccatcgc caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg
    16321 aggatcagcg aacagacggg caccaccacc agatagaggt aatctggcac gatgcgttta
    16381 aggcgagttt caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc
    16441 tgatagccca ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc
    16501 ccgagcagat aagcgttcat cagttggggg gaaaccagtg tcacgccaag cacgatacca
    16561 aggatcggtg tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag
    16621 aagaagatcg cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac
    16681 atttgcgcca gcgtttgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg
    16741 aggatcaaac cgccgctaat caacgcgggc agcaacggga agaaggtctc tgcgaaatga
    16801 gagatcaact gctcatgcca tttcatattc tgccgggcgg ctttttttac ctgctcttta
    16861 tcaacctgcg cctgtccggt tgacgctatc agtgcttgat agtagtcacc cacgttggtg
    16921 ccaatcacca cctgaaattg cctggcattg gtgaaacagc ctttcaccat ggggagttgc
    16981 tcaatttctt tcggtctggc attggccggt tggttgagga caaagcgtag gcgagtaata
    17041 cagtggctca ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata
    17101 tccgtttggt ttattttgct catcataaag ccccatggca gatgacattt ttggttggct
    17161 gcagaatata ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat
    17221 cacaatttat cgttcaggaa acgatctgga cagggtggcg gggatgatga tttgttgcgg
    17281 ttcgctgcgc ccggttacct gtgcgatcaa ctggcaggcc gcctggcgtc cagcttcggc
    17341 gtaaccggga tctacggtga cgatctccgg atggaggaat ttcattaacg gcgtattacc
    17401 gacgctcgcc agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag
    17461 tgccagcgtg tcggttgcgc acagtaaggc ggtagtttca ggcgtaatca cttttgctac
    17521 gttctcatag ccttgcttca tagcaaggcc tggcagagcg gcaacgggat gcagtttatg
    17581 cgctttgcag aacgccaggt aggcttcgtg acgtcgctta ccggtcgtca cgtcactgtg
    17641 cggcacgccg agataactga tattacgatg cccctggtca tacagccgtt gcatcaggat
    17701 cttgattgcc ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag
    17761 cagaaccagc gatgactgcc agtgggctaa catttcttct gttatgccgg taaaaccgaa
    17821 cagcactacg ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg
    17881 cggggaaaac tgactttcca tcatgattgg gtcgtaacct tgttcataga actctggcag
    17941 catggtttga acggcgagat tttctgacaa cgaatccagg cgggtaacaa tgatggcgac
    18001 cactttatcg ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg
    18061 attcatcact gcttcaacac gctcgcgggt gcgctggctc acgccgcttt cgttattcag
    18121 cacccgggaa actgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag
    18181 ccgattttgc atcctgttgt cctgtaacgt gttgtttaat tatttgagcc taactttacc
    18241 cgtgcattca gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta
    18301 tatcgccata gatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc
    18361 acttaccgga ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact
    18421 tttccggtaa gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaac
    18481 gtccctgctc agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt
    18541 actgtttcag acagtgcgga gggactcctt atgtttaaag aaatttttac ccggctcatt
    18601 cgccatttac cttcccgtct ggttcatcgt gatccattgc ctggcgcgca gcagacagtg
    18661 aatacggtgg tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa
    18721 gaagaattgt ggaaaacgtt cgacacccat ccggaagggt taaatcaggc ggaagtggaa
    18781 tctgcccgcg aacaacatgg tgaaaataaa ttacccgcac aacaactgtc gccgtggtgg
    18841 gtacatttat gggtctgcta tcgcaacccc tttaatattt tactcaccat tctcggcgct
    18901 atttcttacg ccacggaaga tttatttgct gcgggcgtta tcgcgctaat ggtcgctatt
    18961 tctacgttgc tgaactttat tcaggaagca cgttccacta aagcggcaga tgccctgaaa
    19021 gcgatggtca gcaataccgc gacggtgctg cgcgtaatta acgacaaagg cgaaaatggc
    19081 tggctggaga tcccgatcga ccagctggtg cccggcgata ttataaaact ggcggcgggg
    19141 gatatgatcc cggcagattt acgtatcttg caagcgcggg atctgttcgt cgctcaggcg
    19201 tcgttaaccg gtgagtctct gcccgtggaa aaagccgcta ccactcgcca gccggagcac
    19261 agcaatccgc tggagtgcga cacgctgtgt tttatgggca ccaccgtggt gagcggcacg
    19321 gcacaagcaa tggtgattgc tacaggtgcc aatacctggt ttggtcaact ggcggggcgt
    19381 gttagtgagc aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgtcagtatg
    19441 ctgctgattc gctttatgct ggtgatggca ccggtggtgc tgttaatcaa tggctacacc
    19501 aaaggcgact ggtgggaagc ggcgctgttt gcgctttcgg tcgcggtagg cctaacgccg
    19561 gaaatgttgc cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa
    19621 cagaaagtga tcgtcaaaca tctggatgct attcagaact ttggcgcaat ggatattctg
    19681 tgcactgata aaaccggcac cctgacgcag gataaaattg tgctggagaa tcataccgat
    19741 atctccggta aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattatcag
    19801 accggactta aaaacctgct cgatacagcg gtgctcgaag gtacggatga agagtcagcg
    19861 cgctcgctgg ccagtcgttg gcaaaaaatc gatgagattc cgtttgattt cgagcgtcgc
    19921 cggatgtcgg tggtagtggc agaaaatacc gagcaccatc agctggtttg caaaggtgca
    19981 ttgcaggaaa tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtgccgctc
    20041 gatgacatca tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg
    20101 cgcgtggttg cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg
    20161 gatgaatccg acctgatcct cgaaggatat attgcttttc ttgatccgcc aaaagagaca
    20221 accgctccgg cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc
    20281 gacagtgagt tagttgcagc gaaagtgtgc catgaagtgg ggctggacgc gggagaggtg
    20341 gtcattggta gtgatattga aacgctatct gacgacgaac tggcaaatct tgcacagcgc
    20401 accacgctgt ttgcccgcct gacgccgatg cataaagaac gcattgtgac cttactgaag
    20461 cgcgaagggc atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc
    20521 gcggcggata tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat
    20581 atcatcctgc tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc
    20641 actttcgcca acatgctgaa atacatcaaa atgacggcga gttctaactt tggtaatgtg
    20701 ttcagcgtgc tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg
    20761 cttattcaga acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac
    20821 gacgagcaaa ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatgatc
    20881 ttcttcggac cgatcagctc gatcttcgat attctgacgt tttgcctgat gtggtgggta
    20941 ttccatgcca acacgccgga aacgcaaacg ctgttccagt cgggatggtt tgtggtgggc
    21001 ttactgtcgc aaacgctgat tgtgcatatg atccgcaccc gccgtgtgcc gtttattcag
    21061 agctgtgcat cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca
    21121 ttgccgtttt caccgctggc cagttatctg caattacagg cgttgccgtt aagctatttc
    21181 ccgtggctgg ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaaggattt
    21241 tatagccgtc gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga
    21301 ttgcagacaa agtgagtttt gttcatgccg gatgcggcgt aaacgcctaa tccggcctac
    21361 aaattcaatc aattgcagga atcacgtagg cctgataagc gtagcgcatc aggcgatttt
    21421 gcgtttgtca tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac
    21481 gaatagcgat cgcttcgatc tcaatcttta cgtctttcgg cagacgggca acttcaacgc
    21541 aagaacgtgc cgggaaggtg gcgttgtgtt cggtgaagaa ggtttcgtaa gtggcgttta
    21601 cggttgcgaa gtcgttcaga tcttttacaa acacggtagt tttaacgatg tcgcccactt
    21661 tcagaccagc ggcttcgacg atcgctttta cgttatccag cgactgacgt gcctgtgcag
    21721 cgacgtctgc cggtacttcg cccgttttcg gatttaccgg gatctgaccg gaggtgatga
    21781 tcatatttcc cagatcaacg ccctgtacgt aaggaccgat agctgctggt gcattttccg
    21841 tcgcgatagt tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta
    21901 tagggagcca gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa
    21961 actctttttc acagtatttg catttgagcg cgatgtcatt ggcgcgtttt cgcacggcaa
    22021 agctggatga aaccggttcg gcatggctga tacagttgct gttcgggcag accaacacat
    22081 tgtcgatgcg ctctggcaga ctggggcgcg atttacccac cacttcatag ttgtcgatac
    22141 ggttaaccgt ggcttgcggc gcatacaatg ccagttgatc tacctgatct tcactcaaaa
    22201 aggtattttc gattttgatc agatctttgc ggcccatctc gccagaaggc aggttcagac
    22261 caatggtgat gcgctgatcc gtttcggtca gcttgaacag actcaacagc ttaaaaccga
    22321 tctgggcggg gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat
    22381 tatcgtgtgt catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca
    22441 gtaacgcctg gcgagcgaaa atcccgttgc ctgcctgctg gaagtaccag gcgtgtggcg
    22501 ttttatcaac atccgtcgca atttcatcaa cacgcggcag cggatgcagt actttcatat
    22561 tggctttggc gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt
    22621 attcggatgg gtccaggcgc tctttttgca cgcgggtcat atacaggatg tctacttccg
    22681 ccatcacttc ttcaatagag ctgtgcagac tccatgcgat ccctttttca tcgagcatat
    22741 ccagaatgta ttgcggcatt gccagcgcgt ccggcgcgat gaagtaaaaa cggttgccgt
    22801 cgaacttcgc taacgcctga gtcagggagt gaacggtgcg gccatatttc aggtcaccaa
    22861 ccattgcgac gtggagattg tccagacgcc cctgggtttc ctgaatagtg aataagtcca
    22921 gcaaggtttg cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc
    22981 cggaaaactc ggtggccagg cgcgccgcac cttcctgcgg atgacgcatc actatcgcat
    23041 cgacgtaagt gctgataacc gaaatggtat cggccagcgt ttcgcccttt ttacccagtg
    23101 atgtattggc gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt
    23161 cgaaagagag gcgggtacgg gtagaggctt cgaagaagca gctggcaatg actttgtgct
    23221 tcaacagctc tggttgcggg tttgctttca gttttgccgc tgttgccagc accagattaa
    23281 ggtcatcgcg actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca
    23341 tcttttatct cctgacgcct gggcaaaaaa aagcccctcg attgaggggc tgggaatggg
    23401 tgatcaacgg gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat
    23461 gtcgaacaca ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatcgtca
    23521 gaagcggtgc aagcaattga tgcaatattt tgtcagcgca aacggtttat ttgaatttaa
    23581 agttaaaata tatgcatttt tatttactgt gattctgggg ggaactgtcg ttcggtatca
    23641 ataattgcag acatttcctg cttttttctt tttcactttc acgcaatcag atatgcattt
    23701 tattccattc atccgactta tagggcggag tgtgaaaacg aacggctagc actattgctt
    23761 agagctcagg gatgcgcgct atcactttaa tttcaaaatc aaagcctgcc agccatgtaa
    23821 cgcccaccgc cgtccaggtt ggataagatg gcgcgctaaa tatttcattt ttcaccgtca
    23881 tgatgtcttc aaattgtttt tctggatcgg tatgaaagct cgtaacatca atgatatcgt
    23941 caaaagtgca tcccgcagct gccagggtcg catgcaaatt atcaaaggcc agtctgactt
    24001 gttgctgaaa atcgggttct ggtgttccgt cctctcgact tcctacttgc ccggaaacaa
    24061 acagcaaatc gccggaacga atagccgcag aataacgatg ctcagcatac agtgaatgtc
    24121 gtccagcagg gaaaacagcg gttctttcta ccatttggtt atcctcaaga tttacgacat
    24181 gaacagaaga tttatattta ccaggagccg cttttagcgg acgacgtgag taaacaaaac
    24241 ccagacatca tgggaaatgg ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg
    24301 ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc taaccatgcg
    24361 accataccag cgacctcttc cggttgccca tgtcttttga tggccataaa actatgcgac
    24421 atatcgcgca ttggcccgtt ggcgggatta gcgtcggtat caattggccc tggctggacg
    24481 acgttgatgg tgatcccacg cggtccgaaa tcacgggcca gcccgcgcgc catgccttgc
    24541 agggcagatt tactggcggc ataagcagcc atgcctgcaa caggcatacg atcgccattc
    24601 acggagccga tgattaagat gcgtccgcct tcgggcatct gccgggcggc ttcaacagag
    24661 gcatggtaag gagcatgaat attgattttg aaaaggcgat caatatcgtc ggcatttaat
    24721 tccagggcat cgccaaagac gccaatacct gcatttacca ccaggatatc caatgcgccg
    24781 ctcttacgaa cgacatcaat aacagcgtct ttgtcagcac tatctgtgaa tactgctgtc
    24841 gctccagtct cttgtgccag gcgttcagcg gcatctttcg accccgcata ggtgaatcgt
    24901 acattggccc catcggtgac gaaacgacgt acgatagcgg caccgattcc acggctacca
    24961 ccgaggatga gaactgtctt acctgtaaaa gcgcccataa ggactccttg atttattatg
    25021 taacatgcat tacaaaactg ttttaacttt ctgtcaacaa gttttgtaat ggtcactaaa
    25081 aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg
    25141 gcaaaagtgc tttttcacca aaaaggtttc gatgctgtca gtgttgctga agttactgat
    25201 tatcttggta ttaacccccc gagcctctac gcggcttttg gcaataaggc tgggttattt
    25261 agccgtgtac tcaatgaata tgtcggtacg gaagctattc cgcttgccga tattcttcgt
    25321 gatgatcgtc cggtagggga gtgcctggct gaggtattaa aagaagcggc gcgccgatat
    25381 agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gcattcatag tcatgatcca
    25441 caagcgcgtg atattgccgt tcaatattat cacgccgcag aaacgaccat ttatgactat
    25501 atcgccaggc ggcatccaca aagcgcacaa tgtgtgactg attttatgag taccgtgatg
    25561 tcagggcttt ctgcgaaggc acgggagggg cactccatcg aacaactctg tgcaacagct
    25621 gcactggcgg gggaagcgat aaaaactatt ctcaaggagt gatgcgggcc ttgacccgaa
    25681 aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgcg ttaaggctag
    25741 cggaattgat catcttttcg tataacgatg gaaatgaaac gttgttttaa ttaaggagtg
    25801 gaaaagatga tcatcggaaa tattcataat cttcagccgt ggctacccca ggagttacgc
    25861 caggcgattg agcatatcaa agcacacgtt acggcagaaa cgccaaaggg caagcacgat
    25921 atcgaaggca atcgtctgtt ttatcttatc tcggaagata tgaccgagcc gcacgaagct
    25981 cgccgtgcgg agtaccatgc ccgctatctc gacattcaga ttgtgttaaa aggtcaggaa
    26041 ggcatgacct tcagcacgca acctgcaggc accccggata ccgactggtt agctgataaa
    26101 gacatcgcat ttttgccgga aggcgttgat gagaaaacag ttatccttaa tgaaggtgat
    26161 tttgttgtgt tttatccggg ggaagtgcat aaaccgctgt gcgcagtggg cgcgccagcc
    26221 cgggttcgca aagcagtagt gaagatgctg atggcgtgat gacttttcgc cgtaaataac
    26281 tccaggttta cggcgagttt gtgaaaagag cgttttttga tatttttttg tgagtaaaat
    26341 ttgtaatgct tagacgttct tattaactca aggagttcgt catgagcaaa atatcagatt
    26401 tgaattattc tcaacacatt acattagccg acaattttaa acaaaaaagt gaagttttaa
    26461 atacctggcg tgttggaatg aataattttg cccgtaatgc cgaggggcag gataacacaa
    26521 gaaatatcct tgatcctaag acatttttgg aatttttggt aaaaatattt actctaggtt
    26581 atgtggattt tagcaaacgc tccaacgaag cgggaagaaa tatgatggct catattgagt
    26641 cctcatctta tatcaaaaat aatgatggca gtgagataat aaagtttgtt atgaataatc
    26701 ctgaagggga acgagcggat ttatctaagg tggaaataga aatcacactt tccactttca
    26761 ctactatggg gactcgtcaa ggacatacgg ctattatatt tcaacaacct gatggttcga
    26821 ctaatcgtta tgaaggaaag tcctttgaaa gaaaagatga gagttcatta cacctgatta
    26881 ctaacaagat tctggcatgt taccaacgcg aagctaacaa ggaaatagct cgtctattaa
    26941 atatttcaca ggagttaaat aattctcagg acttgaataa ctcgcaggtg agttgtaaag
    27001 attcagttga ttctacgatt acggatttat tagaaaaacc attgaataat gcattattag
    27061 caataaggaa agaacatctg ctattaatgc cttatgtatg taatgaatcg atttcatact
    27121 tgcttggtga aaaagggata ctgaaagaaa tagatgacct taacgcagta aataattatc
    27181 ttcttaataa taagaaagct actgacaatg aaattaatga catcaaagtt aacctgtctc
    27241 atattcttat tgattcctta gatgatgcaa aagttaacct tacaccggtc atcgattctg
    27301 gagacttttt taaaatcccc atatattaat gatgtaagaa tactggattg gtgttttaat
    27361 aaaagaatgc aatattttgg tgattctgaa aagataaagt atgcatgctc cgtgataaat
    27421 catattgatt ttagccgcga tcaatctaaa gattttagct gcgatcaatc taaaattaaa
    27481 atagctgaga ccttattttt caatctcgat aaagaaccct ataaaaat
//
LOCUS       DAJTOR010000038        27505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTOR010000038 DAJTOR010000000
VERSION     DAJTOR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.038"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..504)
                     /locus_tag="OO249_001451"
                     /pseudo
     CDS             complement(1..504)
                     /locus_tag="OO249_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
     gene            complement(682..1593)
                     /gene="gsiD"
                     /locus_tag="OO249_001452"
     CDS             complement(682..1593)
                     /gene="gsiD"
                     /locus_tag="OO249_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5635798.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1596..2516)
                     /gene="gsiC"
                     /locus_tag="OO249_001453"
     CDS             complement(1596..2516)
                     /gene="gsiC"
                     /locus_tag="OO249_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5635799.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2534..4072)
                     /gene="gsiB"
                     /locus_tag="OO249_001454"
     CDS             complement(2534..4072)
                     /gene="gsiB"
                     /locus_tag="OO249_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5635800.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILVH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4092..5963)
                     /gene="gsiA"
                     /locus_tag="OO249_001455"
     CDS             complement(4092..5963)
                     /gene="gsiA"
                     /locus_tag="OO249_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5635801.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLCVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5950..6915)
                     /gene="iaaA"
                     /locus_tag="OO249_001456"
     CDS             complement(5950..6915)
                     /gene="iaaA"
                     /locus_tag="OO249_001456"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5635802.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPAIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIGHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7119..8354
                     /gene="moeA"
                     /locus_tag="OO249_001457"
     CDS             7119..8354
                     /gene="moeA"
                     /locus_tag="OO249_001457"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5635803.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8354..9103
                     /gene="moeB"
                     /locus_tag="OO249_001458"
     CDS             8354..9103
                     /gene="moeB"
                     /locus_tag="OO249_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5635804.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHNDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEYDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9179..9841)
                     /gene="fsa"
                     /locus_tag="OO249_001459"
     CDS             complement(9179..9841)
                     /gene="fsa"
                     /locus_tag="OO249_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5635805.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9972..10871
                     /locus_tag="OO249_001460"
     CDS             9972..10871
                     /locus_tag="OO249_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5635806.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            10877..13309
                     /locus_tag="OO249_001461"
     CDS             10877..13309
                     /locus_tag="OO249_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5635807.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13455..14270
                     /gene="ybiV"
                     /locus_tag="OO249_001462"
     CDS             13455..14270
                     /gene="ybiV"
                     /locus_tag="OO249_001462"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5635808.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14422..15687
                     /locus_tag="OO249_001463"
     CDS             14422..15687
                     /locus_tag="OO249_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5635809.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFSREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSTSRPEIYPIYWSQTQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     HEDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15928..17520)
                     /locus_tag="OO249_001464"
     CDS             complement(15928..17520)
                     /locus_tag="OO249_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5635810.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17739..18659
                     /gene="ldtB"
                     /locus_tag="OO249_001465"
     CDS             17739..18659
                     /gene="ldtB"
                     /locus_tag="OO249_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5635811.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18718..19836)
                     /locus_tag="OO249_001466"
     CDS             complement(18718..19836)
                     /locus_tag="OO249_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5635812.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(19833..20300)
                     /gene="mntR"
                     /locus_tag="OO249_001467"
     CDS             complement(19833..20300)
                     /gene="mntR"
                     /locus_tag="OO249_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5635813.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20486..20614
                     /gene="mntS"
                     /locus_tag="OO249_001468"
     CDS             20486..20614
                     /gene="mntS"
                     /locus_tag="OO249_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5635814.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            20886..22469
                     /gene="opgE"
                     /locus_tag="OO249_001469"
     CDS             20886..22469
                     /gene="opgE"
                     /locus_tag="OO249_001469"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5635815.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22518..23033)
                     /gene="ompX"
                     /locus_tag="OO249_001470"
     CDS             complement(22518..23033)
                     /gene="ompX"
                     /locus_tag="OO249_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5635816.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23086..23151)
                     /locus_tag="OO249_001471"
     CDS             complement(23086..23151)
                     /locus_tag="OO249_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635817.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23386..24273
                     /gene="rhtA"
                     /locus_tag="OO249_001472"
     CDS             23386..24273
                     /gene="rhtA"
                     /locus_tag="OO249_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5635818.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24572..25075
                     /gene="dps"
                     /locus_tag="OO249_001473"
                     /gene_synonym="pexB"
     CDS             24572..25075
                     /gene="dps"
                     /locus_tag="OO249_001473"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5635819.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25479..26225
                     /gene="glnH"
                     /locus_tag="OO249_001474"
     CDS             25479..26225
                     /gene="glnH"
                     /locus_tag="OO249_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5635820.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26364..27023
                     /gene="glnP"
                     /locus_tag="OO249_001475"
     CDS             26364..27023
                     /gene="glnP"
                     /locus_tag="OO249_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5635821.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27020..>27505
                     /locus_tag="OO249_001476"
     CDS             27020..>27505
                     /locus_tag="OO249_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5635822.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DE"
BASE COUNT         6782 a         7368 c         6817 g         6538 t
ORIGIN      
        1 atcaaagcaa ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca
       61 cggtcgtttc gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg
      121 tccatttact tattcctgat gataaacaac gattttatca ttcgccctcc gtgaaaagag
      181 ataatattta ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat
      241 tttcagaatc acggcacgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt
      301 taatgtcagg gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag
      361 ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat
      421 gaaaaaaaga ccaataaata acagtgctgc caataaaaat aaaattatca gccttatctt
      481 tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat ctgaacgctg
      541 tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa
      601 atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt gattaccctt
      661 tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc
      721 atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg gaaaaacagc
      781 gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc cccactctgg
      841 tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt
      901 gccaatgcgc atggtgaaaa acaccacgat agacgagacg gtcccaggca agatatgacg
      961 caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg
     1021 tttcaacacc agcgtgttgc cgcgcaccag tcgggcaaac gcggggatgg aaaaaatggc
     1081 gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag
     1141 taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc gatcccacca
     1201 gccttcataa tatccagcca acaagcccag caacgtcccg atcgccgcac cgataaacac
     1261 ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc
     1321 gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc
     1381 ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tggccaccac
     1441 aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc gaaatcgtcg
     1501 ccagaattca tgccacggtg tacgcacctg gtcaggtttg accagtggca tggcgtttaa
     1561 caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta
     1621 atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc
     1681 agaatttccg cctgaatcac cgggtaatcg cgcatttcta cggagtcaac gagtaagcgt
     1741 ccaagtccgg gccagttgaa aactttctcc accacgatag aaccaccgag caaaaagcca
     1801 aactgtaagc ccatcatggt cactaccggg atcatcgcgt tacgtagccc gtgtttgagg
     1861 acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa
     1921 acatcgacaa acgacgcgcg ggtaaagcgc gccatcacgg ctgccactgc cgcgccgagc
     1981 gtcagggagg ataaaatgta gtgctgccaa ctgtctgctc ccacggtagg cagccagccc
     2041 agttcaacgg agaatacctg aattaaaagc atccccagcg caaatgccgg aaacgagatc
     2101 cccgacaccg caatggtcat actcaatcga tccggccaac gattacgcca gacggcggcg
     2161 ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccac
     2221 agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga caccatcgac
     2281 aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc
     2341 tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgggcccg
     2401 gcaatcaatc gcgccggatc gccgggcagc atatgcacaa ataaaaacac cagcaccgag
     2461 acgataaaca gcgtcggaat caaccccagt aagcgtttga taacgtaatt aagcattcca
     2521 ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct gaagccggtg tctggcatga
     2581 tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga
     2641 tccacggcga ctcttgccag atgatatcct gcgccgcctt atacaagcgg gtcttttccg
     2701 ccggatcatt agttttcagt gcctgagcca ggaagtcgtc cacctgttta ttgctgtaaa
     2761 atgcggtatt aaacagcgtc ggtggccagt tttgcgaggc gaacagtggc gatagcgccc
     2821 agtccgcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccgctctctt
     2881 tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgcg gtcacctggg
     2941 ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc
     3001 tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca
     3061 gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga
     3121 tacttggcgg taccacacca gtagctggcg ttgcatagcc cgcaaaggca actttcacca
     3181 gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaacggct
     3241 tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc atcaactcga
     3301 tatttttgtt tttctccagc agtgcggcct gctcgtaggg aatggggaaa gcaaactgcg
     3361 cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta
     3421 tgctgtccag tttgggcaat cctggctgcc agtaacccgc gaattttttc accttcacaa
     3481 aatcggtctg attccaggta tccagttcat acggtccggt tcccaccgga tgaaaaccaa
     3541 tctccttgcc atatttttcc agcgctgccg gtgaaatcat cgcggtcgcc ggatggacaa
     3601 gaatattaat aaacgctgag aacggctgtt tgagggtaat ctttaccgtt gtcggatcga
     3661 tcgcctccgt tttggcaata ttcttataca ggttatagcg tttaagatga ttcgccggat
     3721 cgctggcccg gtccagattc gctttcaccg ccgcggcgtt gaaatcggtg ccatcctgga
     3781 atttaattcc ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa acggtataac
     3841 tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa
     3901 acgatttcgc tacggcctga gataacgtgt cattcgcgtc atacggatcg agcgtggtga
     3961 aattcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat gccatcaacg
     4021 ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agctcttgcc attttattct
     4081 ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc
     4141 cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag atgaatattg
     4201 ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc
     4261 ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa gaccgcgcgc
     4321 cgtggaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc
     4381 tctaccacgg ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga
     4441 tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga aacggcttcg
     4501 tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc
     4561 tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag gcccacgcgc
     4621 tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc gtgtacacac
     4681 agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc
     4741 tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccaggtga caaggtatcg
     4801 attcgctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc
     4861 cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcgccaggc
     4921 cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa
     4981 ccgctgcgca aagggaaacg ggtgacaaga ttacgcactc gtaaaacagg ttcgccatcc
     5041 accaccgttt tctgctcgat ggggggttcc tgtttcgctg gatgttcaag cgatatcaat
     5101 gggaaacgtc ggggataatc taaccctttc atcgcaccaa gttgcggaac agcagctaac
     5161 agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt acccgtttcc
     5221 accgcctcgc cctgatacat caccagtacc cgatcggcta tctctgccac cacgcccata
     5281 tcgtgcgtga taaagataac gcccatcgac atctcttttt gcaatacttt gattaattgc
     5341 aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc aatcagcacc
     5401 gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt
     5461 tgatgcggat aacgtgaaag aatggtttgt gcctcaggaa tgcgtacctg atccagcatc
     5521 cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aattgattcg
     5581 gcaatctgtt cacccacagt aaataccggg ttcagcgatg tcatcggctc ctgaaatatc
     5641 atcgccatat ccgcaccgcg aacatggcgc atttgtgcag cgctctgctc gctaagttca
     5701 atcacttcgc gactgcgccg ctgcaacagc attttatcgc actgtactaa accgcccgcc
     5761 tgttccaaca ggcgcatcaa tgccaacgca gtcactgact taccggagcc ggattcgcca
     5821 acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac cgcagctatt
     5881 ttctgctggt cctgcataaa ggcaatattc agattttcaa ccgccagcac attaccggca
     5941 tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg
     6001 cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac
     6061 attcccttca tggccgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc
     6121 cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc
     6181 gatgtcatat gccgccagcg cgcggatgaa gacttcgccc gtgccggtac aagaaaccgc
     6241 cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc caactcgtcc
     6301 gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccaaattgc cgtctaaatc
     6361 caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg
     6421 gtcgaggacg gttgcccctt ccttgcgcgc tgccagtagt tgttcataac gcaaaggcgt
     6481 ggagaaaatc gccggcgaga cgcgctccat gccacgagca aacgcaaaat tttctgcccc
     6541 ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg caagaaccgg
     6601 attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac
     6661 acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc cggcgttaaa
     6721 cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc
     6781 cgcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcaa tgtagcgtaa
     6841 ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat
     6901 gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat
     6961 cagaattttt atgaattact cattcaattt ttgaatatag aaagatggat atggcatgta
     7021 aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tgtggcgttt ttctgacata
     7081 ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga
     7141 tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt caccccactg accgcccagg
     7201 aaacgctgcc actggtacag tgttttggtc gtattctggc gagcgatgtc gtttcgccgc
     7261 ttgatgttcc ggggtttgat aactccgcaa tggacggcta cgcggtgcgt ttagccgata
     7321 ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccgtatcatg
     7381 gtgaatggcc tgcgggcacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct
     7441 gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg
     7501 ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg
     7561 ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt gcttcactgg
     7621 ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg
     7681 aactccagtt gcccggtcag ccgctgggtg acggccaaat ctacgacacc aaccgtctcg
     7741 ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg attatccgcg
     7801 acgatcccca tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtagtga
     7861 tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaacgatt cttgaagagc
     7921 tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc gcgttcggta
     7981 aactcagcaa tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct
     8041 tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc agcggcctgc
     8101 ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga cgtcttgatt
     8161 tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg accaccggac
     8221 atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc gtgctggaac
     8281 gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt agaaccgttt aacgcgttgt
     8341 tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca accggcaaat
     8401 cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc gtgtgctgat
     8461 cgtcggcctc ggtggactcg gctgcgcagc ttcgcagtat ctggcaagcg ccggtgtcgg
     8521 caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac gccagacact
     8581 gcacaatgat gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg ccctgacgcg
     8641 gatcaaccca catatcgcga ttacgccagt caatgcactg ctagatgacg cagaacttgc
     8701 agcattgatt gctgaatacg atctggtgct cgactgtacg gataacgttg cggtacgtaa
     8761 tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg
     8821 tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcgctgcct
     8881 tagccgtttg tttggcgaga acgcgttaac ctgcgtagaa gcaggcgtga tggcaccgtt
     8941 gatcggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg caggttatgg
     9001 caaaccggcc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt ttcgcgaaat
     9061 gaaactgatg cgtaatccgg ggtgtgaggt gtgcgggcag taattgccac ctccaggcca
     9121 gataagacgc ggcactccgc cacgtcatca gcaatttatg aggatgcaga acgtccggtt
     9181 aaatcgacgt tctgccaaac gctcccagcc agtcctgttc aaacttcgcc acagcggcat
     9241 caaccgccgg atagctaatc atctgttgtg ccacatccag tggcagcgta attgattcac
     9301 atcctgccag taagcagtcc agcgcctgac gcgggttttt gaaactcgct gccagcactt
     9361 tcgcctgcgg cgcatgcatt ttcaataact ggtgtaagtc ggtcacagtc tgaatgccgc
     9421 taccgccctg agcatcaata cgattaacgt aaggcgcaac atattccgca cctgccagcg
     9481 ccgacagcag cccttgtgct gcgccatata ccgcggttcc cagcgttgga atcccttccg
     9541 cttttaacat cttaatagct gccagcccct cggcggtcac cggaactttc accacgatat
     9601 ccgcaataat agaacgcaat ttacgtgcgt cattcaccat cccttcagca gtggtagcca
     9661 ttacctgggc aaacagacgc ccctgaccgc ccatcgcttc atgaagttgc ggaagcacaa
     9721 catccagcgg ttttttaccc gcggcgataa tgcttgggtt agtggtcaca cccgccagcg
     9781 gaaaaatacg tgacagcgcc ttcactgcaa caacgtctga agtatccaga tacagttcca
     9841 taaccatcct caaaatgtac tttttgtctg aatgtagact atcacgggaa aactctctga
     9901 acagttgacg cacatcaatc caactttcat tcgaaagtat tttaatgttt gaacgaaaca
     9961 gagaggcaac tatgattttc aatattcagc gctactcgac ccatgatggc cccggtatcc
    10021 gcacggtcgt atttcttaaa ggctgttcgc tgggctgccg ctggtgtcag aacccggaaa
    10081 gccgcgcccg cacgcaggat ctgctgtatg acgcacgact gtgtctggaa ggctgcgagc
    10141 tgtgcgctaa ggcagcgccg gaagtgattg agcgcgcatt gaatggtttg cttattcatc
    10201 gggaaaagtt aaccccggag catctgacgg cgctaaccga ctgctgcccg acacaggcgt
    10261 taaccgtgtg tggggaagtg aaaagcgttg aggagatcat ggcgaccgtt ctgcgcgata
    10321 aaccgtttta cgatcgcagc ggcggcggct taacgctttc gggtggtgaa ccctttatgc
    10381 agccggaaat agcgacggcg ctactgcaag cctgccacga ggcaggcatt catactgcgg
    10441 tagaaacctg tctgcatgtg ccgtggaaat atatcacccc ttctctgccc tatatcgatc
    10501 tgtttcttgc cgatttaaaa cacattgccg acgcgccgtt taaacagtgg accgacggta
    10561 acgccgccaa ggtgctggat aacctgaaaa aactcgccgc agcgggcaaa aaaatcatta
    10621 tccgcgtgcc gctgattcag ggctttaatg ccgacgaaac ctctgtaaaa gccattaccg
    10681 attttgccgc cgacgagctg cacgttggcg aaattcattt tctgccctac cacacgctgg
    10741 gcatcaacaa atatcactta cttaatctgc cctatgacgc cccggaaaaa ccgcttgatg
    10801 cgccagacct gctcgacttt gcccagcagt atgcctgcca gaaagggtta accgcgacct
    10861 tacgaggata acaaccatga ccacactgaa actggacacg ctcagcgacc gcattaaagc
    10921 gcacaaaaat gcgctggtgc atattgtgaa accgccggtc tgtaccgagc gcgcgcagca
    10981 ctataccgag atgtatcaac aacatctcga taagccgatc ccggtacgtc gcgcgctggc
    11041 tctggcgcat cacctggcga atcgcaccat ctggatcaag cacgatgagt tgatcatcgg
    11101 caaccaggca agcgaggttc gcgctgcgcc gatcttcccg gaatataccg tctcgtggat
    11161 cgaaaaagag attgatgatc tggcagatcg tcccggtgct ggttttgcgg tgagcgaaga
    11221 gaacaaacgc gttctgcatg aagtgtgccc gtggtggcgc ggtcagacgg tacaggatcg
    11281 ctgctacggc atgtttaccg atgagcaaaa aggtctgctg gcgaccggaa tcattaaagc
    11341 ggaaggcaat atgacctccg gcgatgcgca cctggcggtg aatttcccgc tgctgctgga
    11401 aaaaggtctt gatggtctgc gcgagaaagt agcggaacgt cgctcgcgca tcaatctgac
    11461 ggtactggaa gatttgcacg gcgaacagtt cctgaaagcg attgatatcg tgctggtggc
    11521 agtcagcgaa cacattgaac gttttgcagc cctggcgcgt gaaatggccg cgaccgaaac
    11581 ccgcgaaagc cgtcgcgatg aactgctggc gatagcagaa aactgcgatc ttatcgccca
    11641 ccagccgccg cagactttct ggcaggcgct gcaactgtgt tacttcatcc agttgatatt
    11701 gcagatcgaa tctaacggtc actcggtatc gtttggtcgt atggaccagt atctctaccc
    11761 gtactatcgc cgagacgttg aactcaacca gacactggat cgcgaacacg ccatcgaatt
    11821 gctgcatagc tgctggctga aactgctgga agtgaacaag atccgctccg gctcacactc
    11881 aaaagcctct gcgggaagtc ctctgtatca gaacgtcacc attggcggac aaactctggt
    11941 tgatggtcaa ccaatggacg cggtgaatcc actctcttac gcgatcctcg aatcctgcgg
    12001 tcgcctgcgt tccactcagc ctaacctcag cgtgcgttac catgcaggaa tgagtaatga
    12061 tttcctcgac gcctgcgtac aggtgatccg ttgcggcttc gggatgccgg cgttcaacaa
    12121 cgacgaaatc gtgatcccgg aatttattaa actcggtatt gaaccgcagg acgcttacga
    12181 ctacgcagcg attggttgta tcgaaaccgc cgtcggtggc aaatggggct atcgctgtac
    12241 cggcatgagc tttatcaact tcgcccgcgt gatgctggcg gcgctggaag gcgggcgtga
    12301 tgccacaagc ggcaacgtgt tcctgccaca agaaaaagcg ttgtcggcag gcaactttaa
    12361 caatttcgat gaagtaatgg acgcgtggga tacgcaaatc cgttattaca cgcgtaaatc
    12421 aatcgaaatc gaatatgtcg tcgacaccat gctggaagag aacgtgcacg atattctctg
    12481 ctcggcgctg gtggatgact gtattgagcg agcgaaaagt atcaagcaag gcggcgcgaa
    12541 atatgactgg gtttctggcc tgcaggttgg cattgccaac ctcggcaaca gcctggcggc
    12601 agtgaagaaa ctggtatttg aacagggcgc gattggtcag caacagctgg ctgccgcact
    12661 ggcggatgac ttcgatggcc tgactcatga gcaactgcgt cagcggctga ttaacggtgc
    12721 gccgaagtac ggcaacgacg atgatactgt cgatacgctg ctggctcgcg cttatcagac
    12781 ctatatcgac gaactgaaac agtaccataa tccgcgctac ggtcgtggtc cggttggcgg
    12841 caactattac gcgggtacgt catctatctc cgctaacgta ccgtttggtg cgcaaactat
    12901 ggcaacgccg gacgggcgta aagcccacac cccgctggca gaaggcgcta gtccggcctc
    12961 tggtactgac catctcggcc ctactgcggt cattggctcg gtgggtaaac tgcctacggc
    13021 agcgattctc ggcggcgtgt tgctcaacca gaaactgaat ccggcaacgc tggagaacga
    13081 atctgacaag cagaagctga tgatcctgct gcgcaccttc ttcgaggtgc ataaaggctg
    13141 gcatattcag tacaacatcg tttcccgcga aacgctgctg gaggcgaaaa aacatccgga
    13201 tcagtaccgc gatctggtag tgcgtgtcgc gggttattcc gccttcttca ccgcgctctc
    13261 tccagacgct caggacgata tcatcgctcg tactgaacat atgctgtaat ctcttcggcc
    13321 cggctgcggc gtcgccgggc taaatcattc acatcatcaa tttcatcctt actttcattc
    13381 gaaatataat ttgtgctctg cgtcacattg ttattacact gttacggttt cattttcgag
    13441 ccaggagcac acctatgagc gtaaaagtta tcgtcacaga catggacggt acttttctta
    13501 acgacgccaa aacgtacaac caaccacgtt ttatggcgca atatcaggaa ctgaaaaagc
    13561 gcggcattga gttcgttgtc gccagcggta atcagtatta ccagcttatt tcgttctttc
    13621 ctgagctaaa ggatgagatc tcttttgtcg cggaaaacgg cgcactggtt tacgaacatg
    13681 gcaagcaact gttccacggc gaactgaccc gacatgaatc gcggattgtt attggcgagt
    13741 tgctaaaaga taagcaactc aattttgtcg cctgcggtct gcaaagtgca tatgtcagcg
    13801 aaaatgcccc cgaagcattt gtcgcactga tggcaaaaca ctaccatcgc ctgaaagctg
    13861 taaaagatta tcaggagatt gacgacgtac tgttcaagtt ttcgctcaac ctgccagatg
    13921 aacaaatccc gttagtgatc gacaaactgc acatagcgtt cgatggcatt atgaaacccg
    13981 tcaccagtgg ttttggcttt atcgacctga ttattcccgg tctacataaa gcaaacggta
    14041 tttcgcggtt actgaaacgc tgggatctgt caccgcaaaa tgtggtagcg attggcgaca
    14101 gcggtaacga tgcggagatg ctgaaaatgg cgcgttattc ctttgcgatg ggcaatgctg
    14161 cggaaaacat taaacaaatc gcccgttacg ctaccgatga taataatcat gaaggcgcgc
    14221 tgaatgtgat tcaggcggtg ctggataaca catccccttt taacagctga cctctctacc
    14281 ggagccgtgc gctccggttt tcccttcttt ttacatcctg tacatcacat caaatcatta
    14341 aacttgcatt aacttattta taacttatat tgtcacatgc aaatcataat ggtttcgaat
    14401 gaaagttaat gagaggtggt tatggcttct acttttacca gcgacacatt gccagccgat
    14461 cacaaagcag ctatccgtca gatgaagcac gcgctgcggg cgcagcttgg cgacgtccag
    14521 cagatcttta atcagctaag cgatgacatt gccacgcgag tggctgaaat caacgcactc
    14581 aaagcacagg gcgatgccgt ctggccggtg ctgtcttatg ccgatatcaa agcaggtcat
    14641 gttactgcag agcagcgcga acagattaaa cgtcgcggtt gtgcggtgat aaaaggccat
    14701 ttctcccgcg aacaagcgct aggctgggat cagtcgatgc tggactatct ggaccgcaac
    14761 cgctttgacg aggtctacaa aggccccggc gataatttct tcgggacgct cagcacttca
    14821 cgtcccgaga tttaccccat ctactggtcg cagacgcaaa tgcaggcccg ccagagtgaa
    14881 gaaatggcga atgcgcagtc gtttctcaat cgtctgtgga catttgaaag tgatggaaag
    14941 caatggttta acccggatgt gagcgtcatc taccctgacc gtatccgccg ccgtccgccc
    15001 ggaacgacct ccaaaggtct tggagcgcat accgactccg gggcgctgga acgctggctg
    15061 cttccagcgt atcagcgcgt tttcgccaac gtctttaatg gcaatctggc gcaatatgat
    15121 ccctggcatg cggcacatcg tacggaagtt gaagagtaca cggtggacaa caccaccaaa
    15181 tgttccgtgt ttcggacatt ccagggctgg acagcgctct ctgatatgct gcctggtcag
    15241 gggttgctgc acgttgtgcc cattcctgaa gccatggcgt acgtactgtt acgtccgctg
    15301 cttgatgatg tgccggagga tgaactgtgc ggcgtagcgc ccggaagagt gttgccggta
    15361 tcagagcaat ggcatccact gttaattgag gcgttaacca gcattccaaa actcgaggcc
    15421 ggagactccg tctggtggca ctgcgacgtc atccattccg ttgcccccgt tgaaaatcaa
    15481 cagggttggg gcaacgtgat gtacattcct gcggcaccga tgtgcgagaa aaatcttgcc
    15541 tacgcgcaca aggtgaaggc cgcactggaa aaaggcgcat cgccgggcga cttcccgcac
    15601 gaggactatg aaacaaactg ggaaggacgc tttacgcttg ccgacctcaa cattcacggt
    15661 aagcgagcgt tgggcatgga tgtttgagag ttaatggaag tgaattgatg tgggattaag
    15721 aaaatcgatt cgacgttact cagattgctg acaagtgcgc tttcttaatg cctgatgcgg
    15781 cgttaacgcc ttatctggcc tacaaagtcc tgcagattca atatattaca ggtactgcgt
    15841 aggcctgata agcgtagcgc atcaggctat tttgcatttg tcatcagccg ccgtcacccc
    15901 gacagtatcg gggtgacgga taatcactta ctcgatccct ttactacgca ggtaatcttc
    15961 gtaattaccg ctaaagtcga tcacgcgttc cggggtgatt tccagaatgc gggtcgccag
    16021 ggagcttacg aactcacggt cgtgtgaaac aaagatcagc gtgccctgat acagttccag
    16081 cgccatgttc agtgactcga tggattccat atccaggtgg ttggtcggtt cgtccatgat
    16141 cagaatgttc ggcttctgca tcattaactt accaaacagc atccgccctt tttccccacc
    16201 ggaaagcact ttagctggct ttttgatgtc gtcctggctg aacagcaaac gaccgagaat
    16261 gctgcgtacc gcctgctcgt catcgccttc ctgcttccac tgactcatcc attcgaacac
    16321 ggtcagatca ttttcaaact catattcgtg gtcctgggcg tagtaaccaa tgcgcgcgtt
    16381 ttcagaccat tttacggtgc cgctgtccgg ttgcagatcg cccaccagcg ttttcatcag
    16441 cgttgattta ccgacgccgt tggtacccag taccgcaagt ttttcaccga cctccagcag
    16501 caggttgaga tttttaaaca gtggaccgtt atcaaaccct ttggtcagac cttccacttc
    16561 cagcgcgtta cggaacagtt tcttatcctg ttcaaaacgg atgaacgggt tctgacggct
    16621 ggaggctttc acctcttcca gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg
    16681 gcgagatttc gaggcgttgg cgctaaagcg gctaacgaaa gattgcaact cagcaatctg
    16741 cgctttcttc ttggcgttat cggccagcag acgttcacgc gcctgggtcg ccgccgtcat
    16801 gtactcatcg tagttacccg gataaacgcg cagctcgccg taatccagat ccgccatgtg
    16861 ggtacagacc atgttaagga agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg
    16921 ctcgttcagc acctgttcca gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc
    16981 gtcgagcagg agaatatccg gatcagcaaa cagcgcctgc gccagaagca cacgcagctt
    17041 ccagccagga gcaacttcac tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc
    17101 aagcaacaat tcaccggcgc gagcttccgc agagtaaccg tccatttcgc cgtatttaac
    17161 ttccagatcg gccactttat agccgtcttc ctcactcatt tccggcaaag catagatgcg
    17221 gtcacgctcc tgcttcactt cccacaactc tttatgcccc atgatcaccg tatccagcac
    17281 agtgaactct tcaaaggcaa actgatcctg acgcagctta ccaatgcgct cgttgggatc
    17341 gagggaaacg ttacccaaag tcggctctaa gtcgccgccg aggatcttca taaaggtgga
    17401 tttaccacta ccgttcgcgc caatcaggcc gtaacggttg ccgccgccaa atttgacgga
    17461 aatgttttca aacaacggct tactgccgaa ctgcatggtg acgttactgg aaactaacac
    17521 aggcgtatcc tgaaaagaga tatgacaaac cgcgcattat gccataagtc tgaaagaaga
    17581 tcctgctcct cgcttaaggt ctacactcaa gccaaaggca aaaaatgtga tttcgtacac
    17641 atctgatttc actgtgagct ggaatgaact tataatgcgc ttccaatact ctaatattct
    17701 caacccaatg gcctgccagg cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat
    17761 tattcgcagc ggccttcgct gttgtcggct tttgcagtac cgcctctgcg gtaacttatc
    17821 ctctgccaac cgacgggagt cgcctggttg gtcagaatca ggtgatcacc attcctgaag
    17881 gtaacactca gccgctggag tattttgccg cggagtacca gatggggctt tccaatatga
    17941 tggaagcgaa cccgggtgtg gataccttcc tgccgaaagg cggtactgta ctgaacattc
    18001 cgcagcagct gatcctgccg gataccgttc atgaaggcat cgtcattaac agtgcagaga
    18061 tgcgtctgta ttactatccg aaagggacca acaccgttat cgtgctgccg atcggcattg
    18121 gtcagttagg caaagatacg cctatcaact ggaccaccaa agttgagcgt aagaaagcag
    18181 gcccgacctg gacgccgacc gcaaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc
    18241 ttccggctgt cgttccggca ggtccggata acccgatggg tctgtatgca ctctacatcg
    18301 gtcgcctgta tgctatccat ggcaccaacg ccaacttcgg tatcggcctg cgtgtaagtc
    18361 atggttgtgt gcgtctgcgt aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg
    18421 gtacccgcgt acagtttatt gatgagccgg taaaagcgac caccgagcca gacggcagcc
    18481 gttatattga agtccataac ccgctgtcta ccaccgaagc ccagtttgaa ggtcaggaaa
    18541 ttgtgccaat taccctgacg aagagcgtgc agacagtgac cggtcagcca gatgttgacc
    18601 aggttgttct tgatgaagcg attaaaaacc gctccgggat gccggttcgt ctgaattaat
    18661 cttcaaaact taaagcaaaa ggcggactca taatccgcct tttttatttg ccagacccta
    18721 gttggccggg agtataacta acaaaacata tcccaccaac gccgcccaca acagcatcgg
    18781 tattgaatag agatggaaac gccaccagat gcggcgatcg ttcgccatac gtagcgcaat
    18841 caaacttgcc agcgatccgg gtaataaccc aaagccacct acgtttaccg cccatgccag
    18901 taataaagac ggcggcacat agttcagcaa caatatggta ctcggcacgt tactgatcac
    18961 ctgcgataaa ccgattgccg ttaaccataa cccgggttca gatagatgac tcacgttacc
    19021 caacacgcct tgcaacgctg gaagctgggt cagtaaatgg acgtcgataa acatcgccat
    19081 aaacaccagc agcagcgtcc agtccacact gagcaccacg cgacgtgcca gcagcgcaaa
    19141 gcctgccgcc acaatcacca gtccccacag ctcttgtttg aactccagcg ccgttagaaa
    19201 gacgatatac agccccagac aactccacac cagccgcggt ttccactccg gtgtttgcac
    19261 ccccgtatgg tattgcagca cctttccagg gaaacaacac cagcacagga gcaggagcgt
    19321 cagcatcatt gcgccagcca gcggtgccat ttgggcaata aatccggcaa acgaaagacc
    19381 agaacgtccc cagataagaa tattttgcgg gttgccaatt ggcgtcagta gcgaaccagc
    19441 attgactgcc agcgcctcaa aaataatcag acgattaacc gggatctcac acagtctttt
    19501 tagcgtgata gtcagcggaa caacaataaa cagcgcgaca tcgttggtca gaaaggtaga
    19561 aagcagcgcc gccgccagca ccataaacat cgccagccga cgctccgtag caaagcggcg
    19621 caccattttg cgccccagca catcaaaata accgcttaac tccacacctt tggtcagcag
    19681 catcaggccg cttaaggtga tgatggtgtg ccagtcgata gcagcaggcc aggatttcgg
    19741 tgcaaagggc acgaaaaagc ttaatccgat accaacaaga attaataact gaaaaaaacg
    19801 atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg caccgtgttt ctgggtaaac
    19861 aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct ccatgccttc cgcgtcgcga
    19921 cgggcgattt ccggactgac gcccaacacc aacaagaaat tttcgactat ctgatgtcgc
    19981 tcgcggcttt cctgcgccag cttctctcct tctgccgtta aaaacacgcc acgccagggg
    20041 atcatttcaa tcagccccat ggttgccagc cgcttaagca ttttagccac cgtcggttgc
    20101 gaaactccca gacgagcagc catgtccacc tgacgagctt cccccacttc cctgatcaag
    20161 tcagaaatca gctcaacgta atcatcaata agctcgcgcc gatgcgcctc tctgacctgg
    20221 cggaaccctt caacgtgctc ttccacgttc actaactgcg tcactttttt tgctgttggc
    20281 gtacctgcgc gacgactcat tgtgcttcct cattatggtg acgcatacat agcgttctca
    20341 tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa aaaattaacg ttttagcaat
    20401 agctatataa tatagcctgt gctatatctg tatgtaatgc aatcatccct caaggatcga
    20461 cgggattagc aagtcaggag gtcttatgaa tgagttcaag aggtgtatgc gcgtgtttag
    20521 tcattctccc tttaaagtac ggttaatact gctctctatg ttgtgcgata tggtcaacaa
    20581 caaaccgcag caagataaac cttccgataa atagcggcgt cgcggtacgc cgcttcactc
    20641 ctgctttcat gcaggcataa cgcgttttgg cctgaaaaac cccacttttt gtcggatttg
    20701 caatcccctt cgcaaaagat ttgttcgtca gtagttgacc tgaacggcgg ctcgctctat
    20761 cttcttgcag ccctgcgtat attgcggctc gcggatgcgg acccctttcc actcttcacg
    20821 cactcttgca ggtattgacc cttgacgcca gggtaagcac atggcgtttg ttacgatagt
    20881 ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg tagccgggta tttagcccgt
    20941 ggactgcgtt ctacttttta cagtcgctat taattaacct cggcttaggt taccccttca
    21001 gtttgctcta caccgctgcg tttacggcta ttttgctttt gctatggcga acattgcctc
    21061 gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc ggcttgttat ttcccttttg
    21121 ctcaggccta cggcgcgcct aatttcaata cattgctggc attgcactcc accaatatgg
    21181 aagagtcgac cgaaatcctg acgatttttc cgtggtacag ctacctggtc ggcttattta
    21241 tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga gaatgaaaaa gcgcgctgga
    21301 ataccttcga cagcctgtgt ctggtattca gtgtggcgac attttttgtt gctcccgtgc
    21361 aaaacctggc ctggggtggc gtatttaaac tgaaagatac tggctatccg gtatttcgtt
    21421 ttgctaagga tgtcatcgtc aataataacg aggtgattga agagcaagaa cggatggcaa
    21481 aactttccgg aatgaaagat acctggacgg tcactgccgt taagccgaag tatcagacct
    21541 atgtggtggt gatcggtgaa agcgcgcgtc gcgatgccct cggtgccttt ggcggtcact
    21601 gggacaatac cccgtttgcc agcagcgtta acggtttgat atttgctgac tacattgccg
    21661 ccagtggctc cacgcagaaa tcgcttggct taacgctcaa tcgcgtagtc gatggcaaac
    21721 cacagtttca ggataacttt gtcaccctgg caaatcgcgc gggcttccag acctggtggt
    21781 tttccaacca gggccaaatc ggcgaatacg atactgctat cgccagcatc gccaaacgtg
    21841 cagatgaagt gtacttcctg aaagaaggta attttgaagc agataaaaac acgaaagacg
    21901 aagcgttact ggatatgacc gctcaagtgc tggcgcaaga gcactcgcaa ccgcagctga
    21961 ttgttctgca tctgatgggc tcgcatccgc aggcctgcga caggacacaa ggcaaatacg
    22021 aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta taccatgacg caaacggacg
    22081 atttactgcg caagctgtac gatcagttac gcaacagcgg cagcagcttc tctctggttt
    22141 acttttctga ccacggtctg gcctttaaag agcgcggcaa agacgtgcaa taccttgccc
    22201 atgatgataa atatcagcaa aatttccagg tgccttttat ggtcatttcc agcgacgata
    22261 aagcgcatcg ggtgattaaa gcccgccgct cagccaatga cttcttaggc tttttctcgc
    22321 agtggacggg aattaaagcg aaggaaataa atatcaaata cccgtttata tctgagaaga
    22381 aagccgggcc gatatacatc accaacttcc agttacagaa ggtggattac aaccatctcg
    22441 gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc cgccccgaga ggcggatttt
    22501 ttatatcacc aaagtgatta gaagcggtaa ccaacaccgg caatccaggt gcctacgtca
    22561 acgctacgaa tacggctctg ctcgtaagag aagtccagag caacgttttc catcgggttg
    22621 aactgcagac cagcaccgta ggagaaaccg tagtcgctgg tgtcgtgttt gtaggtcggg
    22681 tattcagtgg tctggaattt accataaccc acacccacta caccgtagat gcttgcccag
    22741 tcgttaatgc ggtaagccgg accagcagtg atgccatagt actggttttt gttgtagtca
    22801 ccagagcttg cagtacgact tttctcggtg taagtgaaag aaccgatcac acccagcggg
    22861 ctgttgtctt cttcatagcg gtatttcagg ttgaaaccgc ccattttgtt catttggccc
    22921 tgagcgtcgc tctgtgcgta accgccagtt acggtagaag tcgcagctac ggaagtacct
    22981 gcggtgaaag ccagaactgc ggccagtgct gaaagacatg caattttttt cataaccacc
    23041 tcaaatgtga ttcaaataag tcctaagttt taaatatatc aaaaattaat gggaaactct
    23101 tcgcgatttg tgatgtctaa cgggccattt catgtaacag aacgtttcca tacaccgcta
    23161 tccatctaaa tttaaatcac tttttcagag aactgcgtaa gtattacgca tgttttccct
    23221 gtcattcatc cagattattc ctaatcacca gactaatgat tccatcaatc ctggcgcatt
    23281 ttagtcaaaa cgggggaaaa ttttttcaac aaatgctcaa ccagcattgg gtatatccag
    23341 tacactccac gctttactta agtctagata tttgtgggag aaaggatgcc tggttcatta
    23401 cgtaaaatgc cggtctggtt accaatagtc atattgctcg ttgccatggc gtctattcag
    23461 ggtggagcct cgttagctaa gtcacttttt cctctggtgg gcgcaccggg tgtcactgcg
    23521 ctgcgtctgg cattaggaac gctgatcctc atcgcgttct ttaagccatg gcgactgcgc
    23581 tttgccaaag agcaacggtt accgctgttg ttttacggcg tttcgctggg tgggatgaat
    23641 tatctttttt atctttctat tcagacagta ccgctgggta ttgcggtggc gctggagttc
    23701 accggaccac tggcggtggc gctgttctct tctcgtcgcc cggtagattt cgtctgggtt
    23761 gtgctggcgg ttcttggtct gtggttcctg ctaccgctgg ggcaagacgt ttcccatgtc
    23821 gatttaaccg gctgtgcgct ggcactgggg gccggggctt gttgggctat ttacatttta
    23881 agtgggcaac gcgcaggagc ggaacatggc cctgcgacgg tggcaattgg ttcgttgatt
    23941 gcagcgttaa ttttcgtgcc aattggagcg cttcaggctg gtgaagcact ctggcactgg
    24001 tcggttattc cattgggtct ggctgtcgct attctctcga ccgctctgcc ttattcgctg
    24061 gaaatgattg ccctcacccg tttgccaaca cggacatttg gtacgctgat gagcatggaa
    24121 ccggcgctgg ctgccgtttc cgggatgatt ttcctcggag aaacactgac acccatacag
    24181 ctgctggcgc tcggcgctat catcgccgct tcaatggggt ctacgctgac agtacgcaaa
    24241 gagagcaaaa taaaagaatt agacattaat taaatttaca tttctgcatg gttatgcata
    24301 accatgcaga atttctcgct acttttcctc tacaccgtct ttatatatcg aattatgcaa
    24361 aagcatattt attccgaaaa ttcctggcga gcagataaat aagaattgtt cttatcaata
    24421 tatctaactc attgaatctt tattagtttt gtttttcacg cttgttacca ctattagtgt
    24481 gataggaaca gccagaatag cggaacacat agccggtgct atacttagtc tcgttaatta
    24541 ctgggacata acatcaagag gatatgaaat tatgagtacc gctaaattag ttaaatcaaa
    24601 agcgaccaat ctgctttata cccgcaacga tgtctccgac agcgagaaaa aagcaacagt
    24661 agagttgctg aatcgccagg ttatccagtt tattgatctt tctttgatta ccaaacaagc
    24721 gcactggaac atgcgcggcg ctaacttcat tgccgtacat gaaatgctgg atggcttccg
    24781 caccgcactg atcgatcatc tggataccat ggcagaacgt gcagtgcagc tgggcggtgt
    24841 agctctgggg accactcaag ttatcaacag caaaaccccg ctgaaaagtt acccgctgga
    24901 catccacaac gttcaggatc acctgaaaga actggctgac cgttacgcaa tcgtcgctaa
    24961 tgacgtacgc aaagcgattg gcgaagcgaa agatgacgac accgcagata tcctgaccgc
    25021 cgcgtctcgc gacctggata aattcctgtg gtttatcgag tctaacatcg aataaatcca
    25081 tcgctgatgg tgcagaactt tagtacccga taaaagcggc ttcctgacag gaggccgttt
    25141 tgttttgcag cccacctcaa cgcacttatt tagtgcatcc atctgctatc tccagctgat
    25201 taagtaaatt ttttgtatcc acatcatcac acaatcgtta cataaagatt gttttttcat
    25261 caggttttac gctaaataat cactgtgttg agtgcacaat tttagcgcac cagattggtg
    25321 ccccagaatg gtgcatcttc agggtattgc cctataaatc gtgcatcacg tttttgccgc
    25381 atctctaaaa atcaatgagt tgcaaaactg gcacgatttt ttcatatatg tgaatgtcac
    25441 gcaggggatc gtcccgtgga tagaaaaaag gaaatgctat gaagtctgta ttaaaagttt
    25501 cactggctgc actgaccctg gtttttgcgg tttcttctca tgccgcggat aaaaaattag
    25561 ttgtcgcgac ggataccgcc ttcgttccgt ttgaatttaa acagggcgat aaatatgtgg
    25621 gctttgacgt tgatctgtgg gctgccatcg ctaaagagct gaagctggat tacgaactga
    25681 agccgatgga tttcagtggg atcattccgg cactgcaaac caaaaacgtc gatctggcgc
    25741 tggcgggcat taccatcacc gacgagcgta aaaaagcgat cgatttctct gacggctact
    25801 acaaaagcgg cctgttagtg atggtgaaag ctaacaataa cgatgtgaaa agcgtgaaag
    25861 atctcgacgg gaaagtggtt gctgtgaaga gcggtactgg ctccgttgat tacgcgaaag
    25921 caaacatcaa aactaaagat ctgcgtcagt tcccgaacat cgataacgcc tatatggaac
    25981 tgggcaccaa ccgcgcagac gccgttctgc acgatacgcc aaacattctg tacttcatca
    26041 aaaccgccgg taacggtcag ttcaaagcgg taggtgactc tctggaagcg cagcaatacg
    26101 gtattgcgtt cccgaaaggt agcgacgagc tgcgtgacaa agtcaacggc gcgttgaaaa
    26161 ccctgcgcga gaacggaact tacaacgaaa tctacaaaaa atggttcggt actgaaccga
    26221 aataataacg ctacacctgt aaagcgcact ggcagttccc tctcccctct ggggagagga
    26281 ttagggtgag gggcgcaaac ccgctccggg gccattaatt accctgaatt tgattattta
    26341 caccacggta acaggaacaa catatgcagt ttgactggag tgccatctgg cctgccattc
    26401 cgcttctgat tgaaggtgcc aaaatgaccc tgtggatttc ggtcctcggt ctggctggcg
    26461 gtctggtaat cggattgctg gcaggttttg cacgcacctt cggtggttgg atagccaacc
    26521 acgtcgcgct ggtctttatt gaagtgatcc gcggcacacc tatcgtcgtc caggtgatgt
    26581 ttatctattt cgccctgccg atggcgttta acgacttacg catcgaccca tttactgcgg
    26641 cggtggtcac catcatgatc aactccggcg cgtacattgc ggaaatcacg cgtggtgcgg
    26701 tgctgtctat tcacaaaggt tttcgtgaag caggactggc gctcggtctt tcacgttggg
    26761 aaaccattcg ctacgtcatt ttaccgctgg cactgcgtcg tatgctgccg ccgctgggta
    26821 accagtggat catcagcatt aaagacacct cgctgtttat tgttatcggc gtggcggaac
    26881 tgacccgtca ggggcaagaa attattgccg gtaacttccg cgcccttgag atctggagcg
    26941 ccgtggcggt gttctatctg attattaccc tggtgctgag ctttattctg cgtcgtctgg
    27001 aaagaaggat gaaaatcctg tgattgaatt taaaaacgtc tccaagcact ttggcccaac
    27061 ccaggtgctg cacaatatcg atttgaacat tgcccagggc gaagtcgtgg tgattatcgg
    27121 gccgtccggt tccggtaaat cgaccctgct gcgctgcatc aacaaactgg aagaaatcac
    27181 ctccggcgat ctgattgtcg atggcctgaa ggttaacgat ccgaaagttg acgagcgcct
    27241 gattcgccag gaagcaggta tggtgttcca gcagttttac ctcttcccgc atctgacggc
    27301 gctggaaaac gtcatgtttg gtccgctacg cgtgcgtggc gcgaacaaag aagaggcgga
    27361 aaaactggcg cgtgagctgc tggcgaaagt cggtctggca gaacgtgcac atcactaccc
    27421 ttccgaactt tctggtggtc aacagcagcg tgtggcgatt gcccgcgcgc tggcggtgaa
    27481 gccgaaaatg atgctgtttg atgaa
//
LOCUS       DAJTOR010000039        26959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTOR010000039 DAJTOR010000000
VERSION     DAJTOR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.039"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..1089)
                     /gene="eno"
                     /locus_tag="OO249_001477"
     CDS             complement(<1..1089)
                     /gene="eno"
                     /locus_tag="OO249_001477"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012005243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5635823.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAG"
     gene            complement(1177..2814)
                     /gene="pyrG"
                     /locus_tag="OO249_001478"
     CDS             complement(1177..2814)
                     /gene="pyrG"
                     /locus_tag="OO249_001478"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5635824.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3042..3833)
                     /gene="mazG"
                     /locus_tag="OO249_001479"
     CDS             complement(3042..3833)
                     /gene="mazG"
                     /locus_tag="OO249_001479"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5635825.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(3904..4239)
                     /gene="mazF"
                     /locus_tag="OO249_001480"
     CDS             complement(3904..4239)
                     /gene="mazF"
                     /locus_tag="OO249_001480"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR5635826.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4239..4487)
                     /gene="mazE"
                     /locus_tag="OO249_001481"
     CDS             complement(4239..4487)
                     /gene="mazE"
                     /locus_tag="OO249_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR5635827.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4565..6799)
                     /locus_tag="OO249_001482"
     CDS             complement(4565..6799)
                     /locus_tag="OO249_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5635828.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(6847..8148)
                     /gene="rlmD"
                     /locus_tag="OO249_001483"
     CDS             complement(6847..8148)
                     /gene="rlmD"
                     /locus_tag="OO249_001483"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5635829.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8205..10961
                     /gene="barA"
                     /locus_tag="OO249_001484"
     CDS             8205..10961
                     /gene="barA"
                     /locus_tag="OO249_001484"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5635830.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11193..12533)
                     /gene="gudD"
                     /locus_tag="OO249_001485"
     CDS             complement(11193..12533)
                     /gene="gudD"
                     /locus_tag="OO249_001485"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5635831.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLCARDNAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12554..13894)
                     /locus_tag="OO249_001486"
     CDS             complement(12554..13894)
                     /locus_tag="OO249_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5635832.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELHVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(13896..15266)
                     /gene="gudP"
                     /locus_tag="OO249_001487"
     CDS             complement(13896..15266)
                     /gene="gudP"
                     /locus_tag="OO249_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5635833.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(15683..16132)
                     /locus_tag="OO249_001488"
     CDS             complement(15683..16132)
                     /locus_tag="OO249_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5635834.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16150..16932)
                     /gene="truC"
                     /locus_tag="OO249_001489"
     CDS             complement(16150..16932)
                     /gene="truC"
                     /locus_tag="OO249_001489"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5635835.1"
                     /translation="MLEILYQDEWLVSVNKPSGWLVHRSWLDRDEKVVVMQTVRNQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(16932..17261)
                     /locus_tag="OO249_001490"
     CDS             complement(16932..17261)
                     /locus_tag="OO249_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5635836.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(17883..18428)
                     /gene="syd"
                     /locus_tag="OO249_001491"
     CDS             complement(17883..18428)
                     /gene="syd"
                     /locus_tag="OO249_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5635837.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18496..19344
                     /gene="queF"
                     /locus_tag="OO249_001492"
     CDS             18496..19344
                     /gene="queF"
                     /locus_tag="OO249_001492"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5635838.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPKVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19456..20820
                     /gene="ppnN"
                     /locus_tag="OO249_001493"
                     /gene_synonym="ygdH"
     CDS             19456..20820
                     /gene="ppnN"
                     /locus_tag="OO249_001493"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5635839.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKAVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21377..22666
                     /gene="sdaC"
                     /locus_tag="OO249_001494"
     CDS             21377..22666
                     /gene="sdaC"
                     /locus_tag="OO249_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5635840.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22724..24091
                     /gene="sdaB"
                     /locus_tag="OO249_001495"
     CDS             22724..24091
                     /gene="sdaB"
                     /locus_tag="OO249_001495"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5635841.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24203..24958
                     /gene="xni"
                     /locus_tag="OO249_001496"
     CDS             24203..24958
                     /gene="xni"
                     /locus_tag="OO249_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5635842.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25063..26200)
                     /gene="fucO"
                     /locus_tag="OO249_001497"
                     /pseudo
     CDS             complement(25063..26200)
                     /gene="fucO"
                     /locus_tag="OO249_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
     gene            complement(26228..26875)
                     /gene="fucA"
                     /locus_tag="OO249_001498"
     CDS             complement(26228..26875)
                     /gene="fucA"
                     /locus_tag="OO249_001498"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5635843.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIAFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6520 a         7238 c         6494 g         6707 t
ORIGIN      
        1 gccagcatct ttcgccatct tgattgcagc cagagtttcg gtcagagaac cgatctggtt
       61 gaatttgatc aggatggagt tagcgatacc tttttcgata ccttctttca ggatcttggt
      121 gttggttacg aacaggtcgt caccaaccag ctggattttg tcgcccagaa ctttggtctg
      181 gtatgcgaaa ccgtcccagt cagattcgtc cagaccgtct tcgatagaaa cgatcgggta
      241 ctgtttggtc agttcttcca ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc
      301 ttcgccagcc agaacgtatt taccatcttt gtagaattca gaagctgcgc agtccatcgc
      361 cagagtgatg tctttgccca gttcataacc agcagcttta acagcttcag cgataacagc
      421 cagagcttca gcgttggaac ccaggttcgg cgcatagcca ccttcgtcac caacagcagt
      481 gttcatgcct ttcgctttca gaacttttgc caggtgatgg aaaacttcag aacccatgcg
      541 gatggcttct ttcacagttt tcgcgccaac cggctgaatc atgaattcct ggatatcaac
      601 gttgttgtca gcgtgctcac caccgttgat gatgttcatc atcggaaccg gcatagagta
      661 tttgcccgga gtaccgttca gttcagcgat gtgctcgtac agcggcatac ctttagcagc
      721 tgcagcagct ttggcgttag ccagagatac agccaggatt gcgttcgcgc cgaatttgga
      781 tttgttttcg gtgccgtcca ggtcgatcat gatcttgtca atgccagcct gatctttagc
      841 atctttgcca atcagcgcct gagcgatcgg gccgtttacc gcagcaacag ctttggttac
      901 gcctttaccc aggaaacggg atttgtcgcc atcgcgcagt tccagagctt cacgggaacc
      961 agtagaagca cctgacggag cagctgccat accgacgaaa ccaccctcca gatgtacttc
     1021 ggcttcaaca gtcgggttac cacgggagtc gatgatttca cgaccgatga tttttacgat
     1081 tttggacatt aggttttcct caagtcacta gttaaactga aactccagac aaacaacacg
     1141 tacccagggt acgcgttgcc gttctaactt ttttacttac ttcgcctgac gtttctggaa
     1201 ctcgctggcg gctttcacaa agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt
     1261 aaactccgga tggaactggc aagccacgaa ccacgggtga ttcggaactt cgatgatctc
     1321 gaccaactga tcatccccgg aacggcccgc aacgcgcaga cctgcatctt caatctgttt
     1381 caacagcatg ttgttgactt cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt
     1441 gtacagctgg cgaaccaggc tatcgtcaac caactggcac tgctgtgcgc cgagacgcat
     1501 ggtaccgccg agatcgctct tctcgctacg aacttcaacg ttgccgtttt catcgcgcca
     1561 ctcggtaatc agcgccacaa ccgggtactt acagtctggc acaaattccg tagagttggc
     1621 gttctccatg ttggcaacat ggcgagcgta atcaattaac gccacctgca tacccaggca
     1681 aatgcccaga taaggaatat tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc
     1741 tacgccacga tagccgaaac cgccaggtac gaggattgcg tccagacctt taaggatttc
     1801 aacgccgcgc gtttcaacat cttgtgaatc gatcagtttg atgttgacgc tgacacgatt
     1861 cttcagccca ccgtgtttca gtgcttcgat cactgattta taagcatccg gcagttcaat
     1921 gtacttgccg accataccga tggtgacttc acttaccggg ttcgcttctt cgaagataac
     1981 ctgttcccat tcggacagat tcgcttccgg gcagtttaag ctgaatcgtt tacaaatata
     2041 atcgtccagc ccctgagatt tcaacaggcc cggaatttta tagatggaat cgacgtcttt
     2101 cagagaaata accgcttttt ccggaacatt acagaacaat gcaatcttcg cacgttcgtt
     2161 cgccggaaca gcgcgatctg aacgacaaat caggatgtca ggctggatac cgatggagag
     2221 cagctctttt acagagtgct gagtcggttt ggttttgact tcaccagacg ctgccatgta
     2281 cggcaccagc gtcaggtgca taaacagagt gtgctcacgg ccaatttcaa cagccatctg
     2341 gcgaatcgct tcgaggaacg gcaaggattc gatatcacct actgtaccgc cgatttctac
     2401 cagtactacg tcatgacctt cgccaccttc cagcacgcgc tctttgattg cgttagtgat
     2461 gtgcggaata acctgcacgg ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac
     2521 gtcagagtag atacgacccg tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa
     2581 acgctcgtag tgccccaggt ccaggtcggt ttcagcgccg tcttcagtaa cgaacacttc
     2641 cccgtgttgg attgggctca tagtacctgg atcgacgttg atgtacggat ccagtttcat
     2701 gatggtcaca ttgaggccac gggcttcaag aatggctgcg agggaggctg cggcaatgcc
     2761 tttacccaga gaggatacga ccccgccggt cacaaaaata tagttcgttg tcatgctgaa
     2821 cctgagaagt taggttgaaa agacgatgga ataaccagga cgggaaagca gtatacccga
     2881 acatgacctg tgccacaaac tttcattatc cctcctcttc gccagcgcac tattgaaatc
     2941 aggagtgaga aaatagcccc tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca
     3001 tttaaaaaat gacacaaatg gcacttgacc gcgtaattcc cttagagatc aatttcctgc
     3061 cgttttacct gttgccagac ttcttccatt gtttcgaggt caacacctgt catttccagt
     3121 ccacgcgcgg caacaatacg ctccacttcg cgaaaacgac gctcgaattt ttcgttcgct
     3181 ttttgcaatg cgatttctgc tttcgtccct aaatggcgag ccagattaac cgtggcaaac
     3241 agcaggtccc ccatttcctc ctccagttta gcctggtcga caacagcctg ccgcgcttcg
     3301 tacatcacct cgtcgatctc ttcgtagact ttatcaacca ccgggccaag cgtcgtccag
     3361 tcgaagccaa cgttggcgca acgtttctgg attttttgcg cccgcattaa agccggtaaa
     3421 ctacgaggaa tatcgtccag cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg
     3481 atttgctccc aacgggcaag cacttcacta ctgttttcgg cagaactatc agcaaaaaca
     3541 tgcggatggc gacgctctaa tttatcgcta atagcagcgc aaatatcatt aaagtcaaag
     3601 cgcccttctt cctgagccat ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt
     3661 tcaccgcgaa gatcgtcaaa atcttcacgg gcgatggcgt ccagcacttc gtaggtttct
     3721 tcaagggtgt aaggcgcaat ggtggcaaat gtctgctctt tatcccacgg gcagccgttt
     3781 tccggatcgc gcaggcgctg cataatagtg agcaaacgat cgatttgatt cattgaattg
     3841 tcctgaaaat tgcgggtctg tcaggtggaa acctgtgacc agaatagaag tgagttagta
     3901 acactaccca atcagtacgt taattttggc tttaatgagt tgtaattcct ctggggcaac
     3961 tgttcctttc ttcgttgctc ctcttgcccg ccaggcgatt ctttttacct gatcagctaa
     4021 cgctacgcca tcacgttcct gaccggataa aacaacttcg aacggatatc cttttgattg
     4081 cgttgtacaa ggaacacaca gacacatacc tgttttgttg ttgtacatga aaggactcag
     4141 gacaacagct ggacgatgtc cagcttgctc gctacctttt gtcgggtcaa aatcaaccca
     4201 aatcagatcg cccatatcgg gtacgtatcg gcttaccatt accagacttc cttatctttc
     4261 ggctctcccc agtcgatatt ctcgtggagg ttttccggcg tgatgtcgtt gaccagttca
     4321 gcaagcgtaa atacgggctc tttacgcact ggctcaataa ttaatttgcc atccaccagg
     4381 tcaatcttca cttcatcatc aatattcaga ttgagcgcct gcattaacgt agccgggatc
     4441 cgcaccgccg gtgaatttcc ccaacgcttt acgctactgt ggatcatcac cctttcctca
     4501 aaccgctatc atatgtagat acagtatata tcaatctaca ttgtagatac gagcaaattt
     4561 ctgcctaact cccgtgcaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac
     4621 ccagcacgcg gcccagcacc tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca
     4681 gttgctgttt ggtgtcgcta cggctggcaa cgccaagtac gttcaccttc tcgttggcga
     4741 gaatggtcgt gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca
     4801 gcgaatatcc ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat
     4861 gagagcgcag ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgcccct
     4921 gggtaatgaa gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt
     4981 gcatcaggtt gccaacacct tcgactacca cgcgaccgtt atctttactg cggttttgcg
     5041 gcgtgtagct tttttgctga agttgcttca gcgcggcggc gtcctgctct tcggcactcg
     5101 gcttattaaa ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac
     5161 caatcgccgc cagcaactcg tcgacatcat taaagttgta acgcggcagc agatgttttt
     5221 ctgcttcttt caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag
     5281 ccagaatgtt tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac
     5341 ggctggttgt gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt
     5401 tctgggtgat aatttcaatc tggtcgccca tctgcagctg gtaggtgaac ggcacaatgc
     5461 gcccgccaat ttttgccccg atgcagcggt gtccgacatc actgtggatg tggtaagcga
     5521 agtccagcgg cgttgatccc gcaggcaaat caacgacatc acctttcggc gtaaagacat
     5581 acacccggtc gtcaaagacc tggctgcgta cttcgtcgag catttcgccg gaatcagcca
     5641 tctcttcctg ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac
     5701 gtgcgccgcc agctgccgcg ccctctttat atttccagtg cgcagcaaca cccaactctg
     5761 catcttcatg catctgtttg gtgcggattt ggatctcaac ggttttgcca cctggcccca
     5821 gaaccacggt atgaatagac tgataaccgt ttggtttcgg gttagcgaca taatcgtcaa
     5881 actcatccgg caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct
     5941 gtaaacgctc ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt
     6001 tctttttctg cattttgcgc cagatgctgt agatgtgttt cggacggcca tacacttcag
     6061 ctttaacgcc ttcagctttc atctcagcgc gcagatgacc gacaaactct tcgatatagt
     6121 gttcgcggtc gagacggcgt tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt
     6181 gcagataacg gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt
     6241 tcgccaacgg cgcgtagata ttggtgcact cttttgccgc cagtacgcgt tcatcttccg
     6301 gcgcatcttt tacttcgcgc agatgagcaa tacgctccgc cagtttgatg accacgcagc
     6361 gaaaatcatc gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa
     6421 cagaatcagt gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa
     6481 taaggttaac gacagactta ccgacgctct cacgcagcac atcttcgctg actacgttgg
     6541 catcggccag agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg
     6601 agaggatctc caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct
     6661 gcgtctgctg cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg
     6721 taatacccag acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg
     6781 cacttcttac cgcaaccatt gtcctctcct ttagggacca gacctgccga aatcggcaaa
     6841 tcgtaactat ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa
     6901 catatccagc atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc
     6961 ccgagccagc gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat
     7021 aatttgctgc ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa
     7081 gccgtttttc gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt
     7141 cacattctgt aagccattaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac
     7201 accttctaca ccgaccacac tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat
     7261 accgcagaac agatccagta cgcgatcttc aggttgcaca tccagccatt ccaacgcact
     7321 cgctaccatt ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt
     7381 taagcgcaac ccgttagagt cataccaggg catctcacca gagacggttt cgagtatctc
     7441 actatcgggg gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc
     7501 gcgatctgcc gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc
     7561 ctgtaccagt tcaacatgac caagatggcg cattgcttgt aagctgccca ggcatgccct
     7621 gactttgggc agcaatgctt caagttgggg cactaaaatg ggacattgtt taacgtcgac
     7681 aatgtcactg gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta
     7741 gttcagactt aaacgcgcgc gacggcgata gccccaggga acatcggcga tcacttcaga
     7801 gacttcgtgt ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac
     7861 gctggcgtgt tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc
     7921 gcgttccggg ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc
     7981 ttcagtaaca gtaacttccg cgttttcctg cggcaataat ccggggataa atagcgtttt
     8041 gccgttatgt cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat
     8101 gatctgacgc gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt
     8161 ctcaatttaa cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc
     8221 tgcgcgcacg catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga
     8281 gtatattttt cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgcaggtg
     8341 ccagcattat tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg
     8401 aatctatcgg tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga
     8461 tttcggttta tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct
     8521 catcaatgca gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg
     8581 gcgatattat gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc
     8641 ccagtagcga tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc
     8701 ttaaatcggt tcgcttgcag caatataaag agatctttat ttccagcgtg atgatgctgt
     8761 tttgtatcgg tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga
     8821 ttcgcaacat ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg
     8881 aaggatttat gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt
     8941 cgctggctgc ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc
     9001 gtgaaacgct ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg
     9061 cccaggaagc ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta
     9121 caccactgaa tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa
     9181 cgcagcgcga tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta
     9241 atgatgttct cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat
     9301 tcccactacg cagcacgctg gatgaagtcg ttactctgct ggcacattct tctcacgata
     9361 aagggttgga actgacgctc aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc
     9421 cactgcgatt acagcaaatc atcactaacc tggtggggaa tgcaattaaa ttcaccgaga
     9481 atggcaacat tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg
     9541 aagtgcagat tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc
     9601 aggccttccg acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc
     9661 tggtgattac acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc
     9721 cgaatcgcgg ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta
     9781 tcgaagggcc atccacccag tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact
     9841 ccgcagcagc gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata
     9901 gcccaacgtt ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg
     9961 tgaccttccg cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga
    10021 ccgatttcct gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc
    10081 aagatggtat cggcgcgtgt ctgctgaaac cattaacacc tacacgcctg ttgcctgccc
    10141 tgacggaatt ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc
    10201 tggcaatgac agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat
    10261 tgctggaaga tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcggttgaac
    10321 gggcgaaaca gatgccgttc gatttgatct taatggatat tcaaatgccg gacatggatg
    10381 gcattcgggc ctgcgaactc atccaccagc tcccccatca gcaacaaacg ccggttatcg
    10441 cggtaacggc gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg
    10501 attatctggc gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc
    10561 ctggcagcgg tatttcctct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc
    10621 cgaatgcgac cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag
    10681 cgcgcgatat gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg
    10741 aacagctggt tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca
    10801 gttgcggcta tagcggtgtg ccgcgtatga agaatctctg ccaactgatc gaacaacagc
    10861 tacgtagcgg tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga
    10921 tggataatgt cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag
    10981 caatcggctt tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg
    11041 atgcggcgtt aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat
    11101 gatgtagacc tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaagccg
    11161 gctccagata gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta
    11221 tcgaacgtcc agccaggaat cagatactgc attcccatcg cattgtcacg cgcgcaaagc
    11281 ccgtgtttct gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc
    11341 agccccggtt tttccggcac ctgaaccaga ccgcctttga tctcaaacgg ttctttggtc
    11401 aagcgctgat tgccttcctg ccaaatccag tgcgtatcaa tcgcggtgat tttacccggt
    11461 gcagcggcgg caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt
    11521 gaaccccagg tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcatc
    11581 gtccagaaat gcggatccgc cagcgggata tcaacggatt gcagggagag cgtatggccc
    11641 atctgccgcc agtcggtggc gatcatattg gttgcggtcg gcaggccagt tgcgcgacgg
    11701 aactctgcca tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca
    11761 taagccagcg accctttcag gtatttacca attttaatcg cttcgttcag cgaccaggca
    11821 ccgttaggat cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac
    11881 tcggcctctt cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat
    11941 ttttcatacg ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga
    12001 cgcaggcgat accagtcgca tgagtcatcc ggctggcttt gatacggcag cggtgtggct
    12061 ttgcgatcac cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca
    12121 tcgcccagca gggatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc
    12181 gcttctatcc cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc
    12241 ccaccagcat cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca
    12301 cccagcgttt tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg
    12361 ccgccgggaa tttcccctac gccagtgtga ccagaattat ctttgataat cataatatta
    12421 cgcgtaaaga acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc
    12481 accgggatga cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg
    12541 tcctttttca gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg
    12601 ggatcaggta ctgcatcgga cctgcgtcgt tacgcgcacc gccaggcaga cgtttatagg
    12661 cctcatgtgc cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag
    12721 gaacggcaat ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct
    12781 cctgccaaat ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat
    12841 gggtaaacat cgccagagag atatcgaaat ggttattaga atggcagccc caggtcagcc
    12901 cccagtcgtc gcaaagctgc gccacacgga ctgcaccgga aagcgtccag aagtgcggat
    12961 cggcaagtgg aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg
    13021 tggcgatcat gttagtcgcg acgggcaagc cggtcgcccg ccgaaactcc gccataactt
    13081 cacgaccgga gaaaccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat
    13141 tcagcccttt gcatagagaa atggcttcat caagtagcca ggcaccgttg ggatcaacgg
    13201 taatccgcgc atcagggaag cgtttcttca atgcacgaac agtgtcgatt tcttgctcgc
    13261 caggtaacac cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt
    13321 ccgccagacg cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact
    13381 catggttgcc cggcgtattt tccagataag gaagatcggt tttggtccga tcaccgatat
    13441 aaaacagata accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc
    13501 agaccggaac attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca
    13561 cggcattaac gtgcaattca aaagtccagg cacctttgcc gaaggtatca aaatcggctg
    13621 cctgattacc tttatgcacc tgctgaacca ctttattcag gcgcgcaact tcctggccca
    13681 gaaccatcgg aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac
    13741 caatgccggt atgcccggcg ttatcggtga gtaccacaat attgcgggtg aaatatgcgt
    13801 tatgtgcccc accaatatta agcaacatgc tatcgtgccc ggcgaccgga atgactttca
    13861 tatcagtaat aacaggactg gattgtgtcg tcatcttatt gccccgcgac aggtttcagc
    13921 tcgatacgct tgatatcgcc caccagcacc aggtagctca gtaccgcgat taaggcatga
    13981 acaccaacat aaatcagcgc cccattaaag gagccagtcg tgccaacgat ataaccaatt
    14041 gcgattggcg tgacgatgcc agaaatgtta ccgaacatgt tgaacaggcc accggaaaga
    14101 ccgctgatct cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc
    14161 ttaccgaaga aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg
    14221 cagaacacca tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg
    14281 ttcagcgatc ccgtgcggcg catcagccag tcggaaataa tcccacccag cacaccgccg
    14341 ataaaaccgc aaaccgccgg aacggaagcc acaaagcccg ctttcagaat tgacatccca
    14401 cgcgcctgca ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata
    14461 cagtactgac cgatataaac gccgatcatc atccgtgacc cgagcagttg tttgatctgc
    14521 ccccacttca cgctgaacgg cactttaact ttggtgtttt gctgatccat attgatcagc
    14581 gcgccacccg cggcgatgta ctccagctct ttctgattta cccccggatg ttgatttggc
    14641 tcgtggatga ctttcaacca gataaagctg atgacaatcc ccagaccgcc cataaagaag
    14701 aagacgtgtg accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc
    14761 gttgcgaagt attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga
    14821 aaccaggccg caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc
    14881 aggaagcgca gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag
    14941 ccttgcagca aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa
    15001 ccaaaacgat ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag
    15061 gcagagaaca catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa
    15121 ccggcaatag aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc
    15181 accactatcc agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa
    15241 gaactcgttg ttgcactcct gaaaattcgc gttagccacg ctaactctgg actgcgacat
    15301 agccaggaag tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga
    15361 atacggcgcg tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc
    15421 gtgcaacaac acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg
    15481 gtggaatcca cttcacggaa atgaaaacaa gaacaagaaa ggaagggtta aaacgaagaa
    15541 ataaagaaga gtatgaaatg gatcgcttga ctccaggcaa acgccagtaa aaatccgcgc
    15601 tatgaagcag tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga
    15661 catttaccgc tcgctgaaca tatcaggaca acagcgtgcc ccactgttcg acccacggat
    15721 ttgattccgt ttccggttcc gggttctcac tggcgtcaat caacagcatt tcgccaactc
    15781 gctgagcgct ctgttcctgc aacaaggcat cgaactgttt gccgccattg cagaaattta
    15841 cataactgct gtcgccgagc gcaatcacgc cataacgcag attcggctgg aagcccagac
    15901 tatctttgat tccctgaaag agcggcacaa tgctatcagg aaggtcgccc tgcccggtcg
    15961 tggacgtaac caccagaaca tacttatcct gataaggcag ccagtcgctt aattcaggat
    16021 cttcaaatac cgttgctttg tggccctgtg cggtcagaat tgcttctgct tcttccgcca
    16081 ctaacagcga atttccgtac atggtgccga caaaaatacc aatttccgcc ataccgtttt
    16141 ctccctggat taggaactta tctctccatc ctgacccgat ggcgcactga actcaaccct
    16201 ttcattttca ggaagcagac cgcgccagcc aaattgtgat aacgcctgca tccaggtgtc
    16261 gtccaggccc gcgtgaatag tcagcggctc gccagtaaaa ggatgcgtca atgacaactg
    16321 actggcatgt agcattaacc gctggaggcc aaaatgctca gcaccgctgc gattctggcg
    16381 taaatcgcca tgtttgctat cgccaataat cggatgacgc aaatgggcaa gatgtcggcg
    16441 aagctgatgt ttgcgtccgg ttttcggctc cagttccacc aggccgtagc gcgtggtcgg
    16501 gtaacgtccg gtcgctaccg gcatttctac ggtcgccaga ccgcgataat gcgtcactgc
    16561 tggctgcggg cctttatctt cgcgggcaaa tttatcagcg attttgtcca gttcttccac
    16621 cagtggataa tccagcaccg cttcttccat caaccagccg cgcacaatcg catggtaacg
    16681 tttctggatt tggtgctgtt caaactgttg tgccagcagc cgtccggcct cgctggataa
    16741 tcccatcaac aacacaccag aagtgggtcg gtccagacga tgagcagtaa aaacatgctg
    16801 tcctatctgg ttacgcacgg tttgcatgac cactactttc tcgtcgcgat ccagccagct
    16861 acggtgaacc agccagccgg agggtttatt taccgaaacc agccattcat cctgatagag
    16921 tatttccagc attagctcgc atcatccgca aaaagagcat ccagtttttc caactcagcc
    16981 agaataagcg cgtgttgcgg atggtccgtc gccagcgcca tttcataata gggtgcaaca
    17041 gcaaaagcgc ccggtaacgg ctggttgtta tttaacaaat cgtgcattcg cgggatcagc
    17101 acccactgca accactccag tggttccatg gtgtccataa agaacggttg ggtactatta
    17161 aattgatgcg gctggggttc atcgtttcgc cagtgctgat gttcacgcag taacgcttca
    17221 agcgcctgca actggagacg aacgcggtca tgagtggtca taaagcaacc tcaataagaa
    17281 aaactgccgc gaaggatagc aggaataaaa aaagggagca ctgtattcac agcgctcccg
    17341 gttcgtttcg cagcattcca gctacttttg ttgctccctg ctcatccttg acaacttttc
    17401 ctctggcctt gcggccaatc gttcatcctg aactattgct tcctgctcac accaccccga
    17461 tgtgatactt catcctgaag tgtccttggc cttcctgacc agccgaatca tcctgaccgg
    17521 ttctcattct ccatcctgga ggtgtccttt aacgcgtcct gcgtcatcct cttcgcttca
    17581 tccagaagcc tttccctgaa acaccatcct ggtgtgtcct gcagaagtgt catcatcctg
    17641 atgttcactt cgttgtctga ctccctgtcg acgaagatag aatcgtcttt ttcgcgaagt
    17701 cttacaaggc gcttacagac aatgccttaa ggaaaatttc atatgaaagt taagaattaa
    17761 caatcaacat tttgatttta aatgaaaata acgattctta tctttttaag tattcgctta
    17821 tttcctggcg atctcctaca gagtctgtaa gagatctctc acaaccagat ggggtatttg
    17881 gattacagaa gaggcttaag ttgattaagg aattccgcaa gatttgaggc taaatgcgtc
    17941 cgtttacgtg ttcccagcgt ctctttgcac acttcaccgg agagattaca caccgagatg
    18001 acctctaact cttcttccag cgtcgcgata aatagcgtgg gcggcagttt tagccttttc
    18061 tgagtcacca gatgaccaat cagattttcc tgcacccgac ggaagtcgtc ttcactccag
    18121 gtttgcagca atgtcagttt gatatcgcca aactgcgcgt gcatatcccc ggcaaactga
    18181 gtcgtataaa aggtatgaat tgtgggttgt atcacaatat caaaagcgcg ttcaaccgcg
    18241 tttacatttt gttccccggt aaacggctgg ggttgccagt atacggcatc ttcggtagtt
    18301 gaaataatgc acggtgaagg aacaccgtac agttcctcac ttaacggcca gcttttatgc
    18361 tcttcatgcc acgcatcgca gtagcgtgca gtaaaatctt tcagggcttg tgcggtcaaa
    18421 tcgtccacta atttctctct tcacttaaac cagatacact tgtcgtttag tttatctggt
    18481 ttatgacggt gaaacatgtc ttcttatgca aaccatcagg cacttgcggg cctgactctt
    18541 ggaaaatcaa ccgattaccg ggatacctat gacgccagcc tactgcaagg cgttccacgc
    18601 agcctgaatc gcgacccgct gggtctgaaa gcggataacc tgccttttca cggtacggat
    18661 atctggacgc tgtatgaact ttcctggctg aatgcgaaag gtttgccgaa ggtcgctgtc
    18721 ggtcatgttg aacttgatta caccagcgta aatctgattg agtcgaagag ttttaagctc
    18781 tatctcaaca gttttaacca gacgcgtttt aataactggg atgaggtgcg ccagacgctg
    18841 gagcgcgact taagcacttg cgctcagggt aagattagcg tggcgttata tcgtcttgat
    18901 gaactggaag gccagccgat aggtcatttt aatggcactt gcattgatga ccaggatatc
    18961 actatcgata actatgaatt cactactgac tatctggaga atgccacctg tggtgaaaaa
    19021 gtagtggaag agacgcttgt cagccacctg ctgaaatcaa actgcctgat cacccatcaa
    19081 ccagattggg gttcgatcca aattcagtat cgtggacgcc aaattgacag agaaaaactg
    19141 ctgcgttacc tggtctcatt ccgtcatcac aacgagttcc acgaacagtg cgtggaacgc
    19201 atctttaatg acctgttacg cttctgccag ccagaaaaat tgagcgttta cgcacgttat
    19261 acccgtcgtg gcggtctgga cattaacccg tggcgcagta atagcgattt tgtcccatcg
    19321 accacaagac tggttcggca ataaattttt tctcaatttt gcgtgctgga ttcacgcaga
    19381 aggttgtgaa aggtcatcag gcagggctat tgtaatcaaa gggaatgacg atattcgtcc
    19441 cataaggagt ttttcttgat tacacatatt agcccgcttg gctccatgga tatgttgtcg
    19501 cagctggaag tggatatgct taaacgcacc gccagcagcg acctctatca actgtttcgc
    19561 aactgttcac ttgccgtact gaactccggt agtttgaccg ataacagcaa agaattgctg
    19621 tctcgttttg aaaatttcga tattaacgtc ttgcgccgtg aacgcggcgt aaagctggaa
    19681 ctgattaatc ccccggaaga ggcttttgtc gatgggcgaa ttattcgcgc tttgcaggcc
    19741 aacttgttcg cggtcctgcg tgacattctc ttcgtttacg ggcaaatcca taacaccgtt
    19801 cgttttccca acctgaatct cgacaactcc gtccacatca ctaacctggt cttttccatc
    19861 ttgcgtaacg ctcgcgcgct gcatgtgggt gaagcgccaa atatggtggt ctgctggggc
    19921 ggtcactcaa ttaacgaaaa cgagtatttg tatgcccgtc gcgtcggaaa ccagctgggc
    19981 ctgcgtgagc tgaatatctg caccggctgt ggtccgggag cgatggaagc gccgatgaaa
    20041 ggtgctgcgg tcggacacgc gcagcagcgt tacaaagaca gtcgttttat tggtatgaca
    20101 gagccgtcga ttatcgccgc tgaaccgcct aacccgctgg tcaacgaatt gatcatcatg
    20161 ccagatatcg aaaaacgtct ggaagcgttt gtccgtatcg ctcacggtat cattatcttc
    20221 cctggcggtg tgggtacggc agaagagttg ctgtatttgc tgggaatttt aatgaacccg
    20281 gccaacaaag atcaggtttt accattgatc ctcaccggcc cgaaagagag cgccgactac
    20341 ttccgcgtac tggacgagtt tgtcgtgcat acgctgggtg aaaacgcgcg ccgccattac
    20401 cgcatcatca ttgatgacgc cgctgaagtc gctcgtcaga tgaaaaaatc gatgccgctg
    20461 gtgaaagaaa atcgccgcga cacaggcgat gcctacagct ttaactggtc aatgcgcatt
    20521 gcgccagatt tgcaaatgcc gtttgagccg tctcacgaga atatggctaa tctgaagctt
    20581 tacccggatc aacccgttga agtgctagct gccgacctgc gccgtgcgtt ctccggtatt
    20641 gtggcgggta acgtaaaagc agtcggtatt cgcgccattg aagagtttgg tccttacaaa
    20701 atcaacggcg ataaagagat tatgcgtcgt atggacgacc tgctacaggg ttttgttgcc
    20761 cagcatcgta tgaagttgcc aggctcagcc tacatccctt gctacgaaat ctgcacgtaa
    20821 tctcccctcc cccggtgacg tttgttgccg ggttttctcg tttttggtca cttactcatc
    20881 aactcatttc atttgttata tgaatgtttc ttaccaccct cacgcgacaa atatcatcac
    20941 agttaatatg tcatacaatt tatgttgcaa cgcaaacgtt tctctatttt cataaaccgt
    21001 attttatcgc tatgaaaaag aaattatcgc catgattaac taaaagtatt gattttttca
    21061 gttcaaccta catatattgc gcgccctgga agaagtcaga tgtcgtttaa tgggcaaata
    21121 ttgcccttaa attctctttt acttttgatt tacagagtaa agcgttggga taatctatct
    21181 tccaagtaga ttattgtatt tgagatcaag atcactgata gatacataac ttgtgtgtat
    21241 ctttccgccc tcaaattatt acggcggtaa atgattaagc catcgccgat agacagattt
    21301 catttttacg gtcaggcacc ttcccgggct gaactggcta aaagctgaat tatttgcatt
    21361 cctccaggag aaatagatgg aaacgactca aaccagcacg attgcgtcga aagactctcg
    21421 tagtgcctgg cgcaagacag acaccatgtg gatgctgggc ctttacggca cggcaatcgg
    21481 cgcgggcgtg ctgttcctgc caatcaacgc cggtgttggc ggtatgatcc cgctgatcat
    21541 catggctatc cttgcgttcc cgatgacgtt ttttgctcac cgcggcctga ctcgcttcgt
    21601 actgtctggt aaaaacccgg gcgaagacat caccgaggtt gtagaagaac actttggtat
    21661 tggcgcaggt aaactgatta ccctgctcta cttcttcgct atctacccga tcctgctggt
    21721 ttatagcgtg gcaatcacca atacagttga aagcttcatg tctcaccagc tgggtatgac
    21781 gccaccgccg cgtgcgattc tgtcgctgat cctgatcgtg ggtatgatga ccatcgttcg
    21841 cttcggtgag cagatgatcg ttaaagcgat gagtattctg gtgttcccgt ttgttggcgt
    21901 attgatgctg ctggctctgt acctgatccc gcagtggaac ggcgcagcac tggaaacgct
    21961 gtctctggac actgcatctg caaccggaaa cggtctgtgg atgaccctgt ggctggcaat
    22021 tccggtaatg gtgttctcgt tcaaccactc tccgatcatc tcttctttcg ccgttgcgaa
    22081 gcgtgaagag tacggcgata tggcagaaca gaaatgctcg aagatcctgg cattcgcaca
    22141 catcatgatg gtgctgaccg taatgttctt cgtcttcagc tgtgtactga gcctgactcc
    22201 ggcagacctg gctgcggcta aagagcagaa catctcgatt ctgtcttacc tggctaacca
    22261 ctttaacgca ccggttatcg cgtggatggc tccgattatc gcgattatcg ctatcaccaa
    22321 atccttcctc ggccactacc tgggcgcacg tgaaggcttc aacggtatgg tgattaaatc
    22381 tctgcgtggt aaaggtaagt ctatcgaaat caacaagctg aaccgtatca ctgcgctgtt
    22441 catgctggta acgacctgga ttgttgccac cctgaacccg agcatcctgg gtatgattga
    22501 aaccctgggc ggtccaatca tcgcgatgat cctgttcctg atgccgatgt acgcaattca
    22561 gaaagtaccg gcaatgcgta agtacagcgg tcacatcagc aacgtattcg ttgtcgtgat
    22621 gggtctgatt gcaatctccg caatcttcta ctctctgttc agctaagtcc tttcgcgccg
    22681 ctttcgggcg gcgcttcctc cgttttaacg cgatgtattt cctatgatta gcgtattcga
    22741 tattttcaaa atcggcattg gcccttccag ttctcatacc gttggaccaa tgaaagcggg
    22801 taaacaattt accgacgatc tgattgcccg taacctgctt aaagacgtga cccgcgtggt
    22861 ggttgacgtg tacggctcgc tctctctgac cggtaaaggc caccacactg atatcgccat
    22921 tattatgggc ctggcgggta acctgccgga taccgtggat atcgattcca tccctggttt
    22981 tattcaggat gtgaatactc atggtcgcct gatgctggca aacggtcagc atgaagtgga
    23041 gttcccggtt gatcagtgca tgaacttcca cgccgacaac ctttctctgc atgaaaacgg
    23101 tatgcgcatt accgcgctgg cgggcgataa agtcgtttac agccagactt actactctat
    23161 tggcggtggc tttatcgttg atgaagagca ttttggccag caggatagcg caccggttga
    23221 agttccttat ccgtacagtt cagcagccga tctgcaaaaa cattgtcagg aaaccgggct
    23281 gtcactctct ggcctgatga tgaaaaacga gctagcgctg cacagcaaag aagagctgga
    23341 acagcacctg gcgaacgtat gggaagtcat gcgtggcggt attgagcgcg gtatttccac
    23401 cgaaggcgtg ttgcctggca aactgcgcgt tccacgccgt gctgcggcac tacgccggat
    23461 gctggtcagc caggataaaa ccaccactga cccgatggcg gttgttgact ggatcaacat
    23521 gtttgcactg gcagtgaacg aagagaacgc tgctggcgga cgcgtggtga ctgcgccgac
    23581 taacggtgcg tgcgggatta tcccggcagt gctggcgtac tacgacaagt ttatccgcga
    23641 agtgaacgct aactcactgg ctcgttacct gctggtagcc agtgccattg gttctcttta
    23701 taagatgaac gcgtcaattt ctggtgctga agtcggctgc cagggtgaag ttggtgtggc
    23761 gtgctcaatg gcggcggctg gtctggcaga actgttaggt gcaagcccgg cgcaggtgtg
    23821 catcgcggcg gaaatcgcca tggagcacaa cctcggtctg acgtgtgacc cggtcgccgg
    23881 acaggtccag gtgccatgca tcgagcgtaa cgccattgcg gcagtaaaag cggtgaacgc
    23941 cgcacgtatg gcgctacgcc gtaccagcga gccgcgcgtc tgcctcgata aagttatcga
    24001 aaccatgtac gaaacaggta aagatatgaa cgccaagtac cgcgaaacct ctcgcggcgg
    24061 cctggcaatg aagatcgttg cctgcgatta atcgctctcc agaggcctcg ttttgcgagg
    24121 cctcttcccg atttctcatc cagccgtagc ctgttccggc atcgaatgtt acccttatcg
    24181 cctgatcttt aagggggtta tcgtggctgt tcatttgctt attgtcgatg cactgaatct
    24241 tattcgtcgc attcatgccg ttcagggatc gccctgtgtc gaaacctgcc agcatgcgct
    24301 cgatcagctc attatgcaca gccagccaac ccacgcggtc gccgtttttg atgatgaaaa
    24361 ccgcagtagc ggctggcgtc atcagcgttt accagattac aaagcgggtc gaccgccaat
    24421 gccggaagag ttgcacgacg agatgcctgc attacgcgcc gcctttgagc aacgcggcgt
    24481 cccgtgctgg tcaaccagcg gcaacgaagc cgatgactta gccgccacgc tggcggtcaa
    24541 agtgacacag gccgggcatc aggcaacgat tgtttcgaca gataaaggct actgtcagtt
    24601 actttcaccg acattacgta ttcgtgatta cttccagaaa cgttggctgg atgcaccatt
    24661 tatcgataaa gaatttggcg ttcaaccgca gcagttgccc gattactggg gacttgcggg
    24721 gatcagcagt tcaaaggtac cgggtgttgc gggaatcgga ccaaaaagcg ccacgcagct
    24781 gctggtcgag tttcagagtc tggaagggat atatgagaat ctggatgcgg ttgccgaaaa
    24841 gtggcgcaaa aaattagaaa cccataaaga gatggcgttt ctgtgccgcg atattgcccg
    24901 cttacaaacc gatttgcata tcgacggcaa tttacagcaa ttgcggttgg tacggtaacg
    24961 gcgagccgga tgcaaacgat tatcaacaca ttgaatttaa tgcacttata taccggataa
    25021 gacgcggcta gcgtcgtatc cggcattatc acatcagcgc atttaccagg cggtatggta
    25081 aagctctaca atatcctcaa gcgttgcttc acgcgggttg ccaccggtac aaacatcatc
    25141 cagtgccgcc tgcgccagtg ccggaatgtc ttccttgcgt acaccaacat cacgcaaatg
    25201 tggcggaata ccgacatcac ggttgagagc aaacaccgct tcaacagcgg cattacgcgc
    25261 ctcttccagc cactttcacg cccataacgc gcgcgatatc gcggtacttc tcaccggtaa
    25321 agtcagcgtt atagcgcatg acatgcggta acaggatggc gttcgcaaca ccgtgtggag
    25381 tgttgtaaaa cgcgcccagt ggatgcgcca taccatgcac caaccctaac ccaacattcg
    25441 agaagcccat acccgcaaca tactgcccga gcgccatttc ttctccggca tccttatcac
    25501 cagcaaccga tcctcgcagc gccccagcaa tgatttcaat cgctttaatg tgcagtgcat
    25561 cggttagcgc ccacgcgcca cgggtaatat acccctcaat agcatgagtg agcgcatcga
    25621 cacccgtcgc agctttcagc gctggaggca taccatccat catgtcagcg tcaataaacg
    25681 ccacctgcgg gatatcatgc ggatcaacgc aaacaaactt gcgccgtttc tcttcgtcag
    25741 tgatcacgta gttaatggtc acttctgccg cagtacctgc tgtggtagga attgccagaa
    25801 tcggtacact gggtttattg gtcggggaaa gcccttccag gctacgcaca tcggcaaact
    25861 ccgggttgtt gctgataatg ccaatcgctt tacaagtatc ctgtggagaa ccaccaccaa
    25921 tagcgatcag gtaatccgcg ccgctattct ggaatacacc gagcccttct ttgacgacag
    25981 taattgttgg gttgggcact acgccgtcgt aaatcgccca tgccagccct gcagcatcca
    26041 tcttatcggt cactttcgcc accacgccgc attgcaccag cgttttatcg gtgacgatca
    26101 gcgccttctg ataaccacgg cgtttcacct catcggttaa agccccaaca gcaccccgac
    26161 caaaccatgc cgtttcgttc agaatcattc tgttagccat catccttctc cttgttgctt
    26221 tacgaaatta ctcttcaatc cgtaacccat aggttttgaa tttctccagc actacggcaa
    26281 tctcttcatc gctcagcact ggcaccgggt ccgtaatcgc cagggtcgtc aggtaaagtt
    26341 gcgccagcac ttcaacttca tgcgccagcc ataacgcttt ttccagattc acctcacaag
    26401 cgataagccc atgatgttgt aacaaagttg ccttacgatt tttgagagcc agcgcaacat
    26461 gttcagaaag ttcgcgtgtt ccaaaggtcg cataaggcgc gcaaggaata gaattaccac
    26521 ctgccgccgc aatcatgtag tgaatagcgg ggatcggtcg gttaagaatg gaaactgctg
    26581 tgcaatgaac ggcatgattg tgaacaaccg cgttggcatc cggtctgctt tgataggccg
    26641 ccatatggaa acgccattcg cttgagggga gctttccttc ctcatgttta ccgttaccat
    26701 caataaaggc aatatgcgac tccgtcagtt tttcatatgg aatgcctgta ggcgtaatca
    26761 gcatcccatc ctgataacgt acactgacgt tccccgctgt cccctggttc agtcccaggc
    26821 gggtcatttc caggcaagtg tcaataatct gacgagcaag tttatttcgt tccattagct
    26881 acctctctct gattcaaaac agggcaataa tgttgttcct ttcacactat tgaattagcc
    26941 gtttaattac ccaccatat
//
LOCUS       DAJTOR010000040        26719 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTOR010000040 DAJTOR010000000
VERSION     DAJTOR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.040"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..257)
                     /gene="rpnB"
                     /locus_tag="OO249_001499"
     CDS             complement(<1..257)
                     /gene="rpnB"
                     /locus_tag="OO249_001499"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5635844.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQS"
     gene            complement(454..1227)
                     /gene="hisP"
                     /locus_tag="OO249_001500"
     CDS             complement(454..1227)
                     /gene="hisP"
                     /locus_tag="OO249_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5635845.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEAHERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(1235..1951)
                     /locus_tag="OO249_001501"
     CDS             complement(1235..1951)
                     /locus_tag="OO249_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5635846.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGIYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVLHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(1948..2634)
                     /gene="hisQ"
                     /locus_tag="OO249_001502"
     CDS             complement(1948..2634)
                     /gene="hisQ"
                     /locus_tag="OO249_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5635847.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(2724..3506)
                     /gene="hisJ"
                     /locus_tag="OO249_001503"
     CDS             complement(2724..3506)
                     /gene="hisJ"
                     /locus_tag="OO249_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5635848.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(3727..4509)
                     /gene="argT"
                     /locus_tag="OO249_001504"
     CDS             complement(3727..4509)
                     /gene="argT"
                     /locus_tag="OO249_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5635849.1"
                     /translation="MKKSILALSLLVGLSVAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(4775..5344)
                     /gene="ubiX"
                     /locus_tag="OO249_001505"
     CDS             complement(4775..5344)
                     /gene="ubiX"
                     /locus_tag="OO249_001505"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5635850.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAISLPEDLFARWQGA"
     gene            complement(5439..6956)
                     /gene="purF"
                     /locus_tag="OO249_001506"
     CDS             complement(5439..6956)
                     /gene="purF"
                     /locus_tag="OO249_001506"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5635851.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQDYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(6993..7481)
                     /gene="cvpA"
                     /locus_tag="OO249_001507"
     CDS             complement(6993..7481)
                     /gene="cvpA"
                     /locus_tag="OO249_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5635852.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(7740..8402)
                     /gene="dedD"
                     /locus_tag="OO249_001508"
     CDS             complement(7740..8402)
                     /gene="dedD"
                     /locus_tag="OO249_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5635853.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(8392..9660)
                     /gene="folC"
                     /locus_tag="OO249_001509"
     CDS             complement(8392..9660)
                     /gene="folC"
                     /locus_tag="OO249_001509"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5635854.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(9730..10644)
                     /gene="accD"
                     /locus_tag="OO249_001510"
     CDS             complement(9730..10644)
                     /gene="accD"
                     /locus_tag="OO249_001510"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5635855.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVEMGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGSIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(10800..11459)
                     /locus_tag="OO249_001511"
     CDS             complement(10800..11459)
                     /locus_tag="OO249_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5635856.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGMLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(11542..12354)
                     /gene="truA"
                     /locus_tag="OO249_001512"
     CDS             complement(11542..12354)
                     /gene="truA"
                     /locus_tag="OO249_001512"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5635857.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPDDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(12354..13367)
                     /locus_tag="OO249_001513"
     CDS             complement(12354..13367)
                     /locus_tag="OO249_001513"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5635858.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(13433..14569)
                     /gene="pdxB"
                     /locus_tag="OO249_001514"
     CDS             complement(13433..14569)
                     /gene="pdxB"
                     /locus_tag="OO249_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5635859.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIAVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            14668..15663
                     /gene="flk"
                     /locus_tag="OO249_001515"
     CDS             14668..15663
                     /gene="flk"
                     /locus_tag="OO249_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5635860.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(15660..16838)
                     /locus_tag="OO249_001516"
     CDS             complement(15660..16838)
                     /locus_tag="OO249_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5635861.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17140..18360)
                     /gene="fabB"
                     /locus_tag="OO249_001517"
     CDS             complement(17140..18360)
                     /gene="fabB"
                     /locus_tag="OO249_001517"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5635862.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            18519..20525
                     /gene="mnmC"
                     /locus_tag="OO249_001518"
     CDS             18519..20525
                     /gene="mnmC"
                     /locus_tag="OO249_001518"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5635863.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQQDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(20646..20924)
                     /locus_tag="OO249_001519"
     CDS             complement(20646..20924)
                     /locus_tag="OO249_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5635864.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(20958..21506)
                     /gene="epmC"
                     /locus_tag="OO249_001520"
     CDS             complement(20958..21506)
                     /gene="epmC"
                     /locus_tag="OO249_001520"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5635865.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(21506..22315)
                     /locus_tag="OO249_001521"
     CDS             complement(21506..22315)
                     /locus_tag="OO249_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5635866.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFAGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(22315..23139)
                     /gene="mepA"
                     /locus_tag="OO249_001522"
     CDS             complement(22315..23139)
                     /gene="mepA"
                     /locus_tag="OO249_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5635867.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(23143..24228)
                     /gene="aroC"
                     /locus_tag="OO249_001523"
     CDS             complement(23143..24228)
                     /gene="aroC"
                     /locus_tag="OO249_001523"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5635868.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(24263..25195)
                     /gene="prmB"
                     /locus_tag="OO249_001524"
     CDS             complement(24263..25195)
                     /gene="prmB"
                     /locus_tag="OO249_001524"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5635869.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            25361..25912
                     /gene="smrB"
                     /locus_tag="OO249_001525"
     CDS             25361..25912
                     /gene="smrB"
                     /locus_tag="OO249_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5635870.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(25985..26698)
                     /locus_tag="OO249_001526"
     CDS             complement(25985..26698)
                     /locus_tag="OO249_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5635871.1"
                     /translation="MATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSR
                     VSMSACPGLEQYDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDD
                     DFPDYIGPTKVSSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMEN
                     GKLCNGNNFDERGVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDT
                     TSRQPIDSTCRFTYILNMY"
BASE COUNT         6587 a         7305 c         6742 g         6085 t
ORIGIN      
        1 ttactttggt gctctatcac tacataaata taacccactc cctcctgcgt tttcacagac
       61 cacaagaggt cggaataata ttgccgcagg tcttcatcaa taaaactgtt tggttccagt
      121 ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat atcaataaaa
      181 tcccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg cggcgtggaa
      241 gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt taagatgacg cgacaataac
      301 cacgattaac acccaatgca gcaggcagtt tttatctttg gacagcgaat tcagaagtat
      361 ttttagcgga ctgcaacttt tacatttcag cggaagctga ctcgcaaatt ccccatgaaa
      421 atactccggc agcgttagcc accggagagg aaattatttc agcgatccct taaggaactg
      481 ttgcagacga gggctttgcg ggttgccaaa taactgctcc ggcgcgccct cttcttctat
      541 tttcccctga tggaggaaaa tgacatgagt agaaacatgg cgagcaaagc ccatttcgtg
      601 agtcactacc accatggttt tcccctcttc tgccagttgc tgcataatac gcaacacttc
      661 gcctaccagt tcaggatcga gcgccgaggt aggttcatca aacagtaaaa cttccggttc
      721 catcgccagc gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa gatgcaccgg
      781 atatttcccc tgcgcacgtt cgtctatccc gacttttgcc agatacttga ccgcccgctc
      841 gtgcgcttcc tgcttgctca ggcccaacac ctgaatcggc gcttccatga cgttttccag
      901 caccgtcata tggctccaga gattgaagtg ctggaatacc atcgtcaggc gtgtgcgcag
      961 taagcgcagt tgatttttat cggcgacttt gagttgaccg tctttgtcgc gcaccagatt
     1021 gatcgtctgg ccattgacca cgatcgaccc ttcactcggt ttttcgagga agttaatgca
     1081 gcgcagaaag gtacttttcc ccgatcccga cgatccgatg atgcttatta catctccggc
     1141 attcgcttgc agtgagaccc ctttcagcac ttcatgttcg ccgtagcgtt tgtgcaaatc
     1201 gataacgttt aatttattct cggacatcgt attctcagtg cgttgaagaa ggtttcacat
     1261 gctgcaacca gcgtttttcc gctctgcgaa agaggctgat caggacataa gagatgatta
     1321 aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg gcgttaatat
     1381 cgcgggctat tttcagcaga tccggcacag tggccgtaaa cgccagcgca gtagagtgca
     1441 gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca gaaggcaaaa
     1501 taatgcaacg atacatttta aaagtcgaga agccataggc tctggcggct tcaatttccc
     1561 catgcagaac cgaacggatt gccccagcaa aaatctcggt agtgtaagcg caggtgttaa
     1621 gcgtcagcgc cagcacggta cagttcaggc cactgcggaa gaaagcgtta aggaattcgg
     1681 ttcccttaac aatctcaagc gtgtatatgc cggaatagaa caccagcaac tgaacataca
     1741 gcggcgtacc gcgaaaaata taggtaaata accagattgg aaactggatg tatttattac
     1801 tggagacacg accaatcgcc agaaacagcg ccaggactcc gcctatcact accgacaaaa
     1861 taagcagcca cagagtgatc gccacaccag taaagcgata accgtcggtc cacagcagcg
     1921 gtttccagta ttcatgtaag atttcgatca caggtcagcc ctcttcacac ccacggagta
     1981 gcggcgctca aggaacagca gcacaccatt ggaaacagtg gtgaaaacca ggtaaatcac
     2041 gccacagacg atggcgaaat agaacggttc ccaggtactt ttgcctgcca gttgggtggc
     2101 tttgaccaca tcttccaggc cgagtaacga aaccagtgcg gtagatttga ggatcacctg
     2161 ccagttgttg ccaatgcctg gcagggcgta acgcatcatc gccggaaaca tgatccgccg
     2221 aaacacttgc ccacgagtaa aaccgaacgc cgtcgccgcc tctatatgtc ctttcggcac
     2281 tgccataaaa gcaccacgga acgtttcggt aaaataagca ccgtagataa aaccgagagt
     2341 gataatacca gcgaccatcg gatcgatatc aatctgcccg acgcccatcg cctccgtcac
     2401 cgtgtttagc gcaatctgca aaccgaagaa aatcagcagc atcaacacta agtccggcac
     2461 gccacgaatc agcgtggtgt acccttcgaa aatcagcccc gaaagccgat tttgcgagag
     2521 cttaccgcca gcgccaatta aaccgatgat tacagcgagc actacagagc tgatagccag
     2581 ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa aacccatata acatgctgcc
     2641 tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc tccggttacc cgaagggagc
     2701 gggctttctt tcaccgatgg cgattagcca ccataaacat caaaatcgaa gtactttttc
     2761 gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt cagtgcttcg
     2821 cgcagttcgt tatcttcttt acgcaggccc atgccggtcc ctacgccaaa cagtttttca
     2881 tctttaacag acgggccacc gaatttgtaa tctttaccga caggttgttt gaggaaacct
     2941 tcgctggcag cgacctcatc ctggaacgcg gcatcaatac gtccggcagt caggtcagaa
     3001 taaatgttgt cctgcccctg atacgagacg atttcaatgc cttttggtgc ccaatgttca
     3061 ttaccgaacg tctcctgggt ggtgccctgc aatacgccta cccgtttgcc tttcagcgac
     3121 tcgactgtcg gctgaatgtc agaatttttc gccaccacca aacgagaatc ggcagcgtac
     3181 agtttgtcgg tgaaggctat ttcttgctga cgtttttccg taatggaaag cgatgacatg
     3241 atggcgtcaa tcttcttcgc ttttaaggac gggattaacg catccagcgg attttcgaca
     3301 aacgtacatt gcgtattgat gcgtttgcat aattcctttg ccagatcgat atcgaagcca
     3361 accagttcgc cttgtgaatt ttttgattca aatggcgcat aggtcgggtc ggtaccgatg
     3421 cggatgtttt gcggaatcgc agcaaatgcc gcagttgcgc tggagaaggc cagaaccaga
     3481 gagagcgata gcaccagttt tttcataatg tcctcaactg acagtctttt tatagggaat
     3541 atttacaggt ttgttacact tatcgtgcca taaaattgac catcaaggca aaatatcccg
     3601 ccccgttaga tgtattattt ttcattaaaa atgcttaagt atgcatttca atgcaccacg
     3661 atagtgcaac aaacctagcg tgcaccatga tgatcattcc atcaggtaca gcttcccagc
     3721 gacgtatcag tcaccgtaga cattaaagtc gaaatacttt ttcgccatct tgtcgtaggt
     3781 gccgtcctga cgcagctcgc caagcgcctt attgaaggca gccgtcagtt cagcatcatc
     3841 tttacgtagc cctacaccgg tgccatcacc gaagtatttt ttgtctttta ctgatgagcc
     3901 agcaaaggcg aaatctttac cagcaggttg cttgaggaat ccttcgctgg cagcaacttc
     3961 atcttgtaac gcagcatcca gacgtcctgc agccagatcg gaatagacca aatcctggtt
     4021 ggcataggcc accacatcca cgcctttgct acgccaggtc tcgttagcgt aagcttcctg
     4081 ggttgatccc tgcagcacac caacatgttt acctttcagt gaatccagcg ttggctgaat
     4141 cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt cggagaaggc
     4201 aatctcctgc tgacgtttat cggtaatgga aagcgacgaa ataatggcgt cgattttttt
     4261 cgctttcagt gaggggatca gcgcgtcaaa gtcactggca acccaggtac atttcacctg
     4321 catccgtttg cacatctcgt taccgagatc gatatcaaag ccaacaaaat cacctttagc
     4381 atctttcgac gagaacggcg cataggtggt atcggttccg atacgtaccg tctccggtag
     4441 cgccgcatag ctggaagccg ctacggagag accgactaac aaagacagag cgagaatcga
     4501 cttcttcata cataaccctc aagtgaaaat ggctttttta tgttgtgtat tgtgttgtgt
     4561 gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta acattcaacg
     4621 ttaaatatgt taataagacg ttgcattatt gtcctgaagt tgaagatagc aggtatagcg
     4681 gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt tgatcgcggt
     4741 tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag cgggcaaaga
     4801 gatcttcagg aaggcttatt gcaaactggt caagaacacg attaaccgtc tgatttatca
     4861 catcatcaag ggattgcggg cgatgataaa acgccggaac aggaggcata atcaccgcac
     4921 cgatttctgc cgcctgagtc attaaacgca gatggcctaa gtgcaatggt gtttcacgca
     4981 cgcagagcac caacgggcga cgctctttca gcaccacatc tgccgcacgg gtcagtaagc
     5041 catcagtata gctatggaca atgccggaaa gggttttgat tgaacagggt aaaatcacca
     5101 tccccagcgt ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc gcatcgtgcg
     5161 tgacatcagc taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg gataaggtct
     5221 ggcgcgccgc ctggctcatc accagatgcg tttcgatatc tgtgacatcg cgcagaacct
     5281 gtaataagcg cacgccataa atcgcgccgc tggcaccgct gatgcctaca atgagtcgtt
     5341 tcataaatag ttgccctgtc agacttgcag gcagactttg cagtatttcg gcgggagttg
     5401 caagtcaggg tgccagaccg gcaccctcag cgaaggcatc atccttcgtt atgcatttcg
     5461 agattttcca cttcgttctg acgttgcact gctttggcgt catcattacg taacgtatcg
     5521 aggaaatcga ggtagtcctg atcaacatct ttggtgacgt agacgccgtt gaacaccgag
     5581 cattcaaact gctggatatc cggattttca gcgcgaacgg cgtcgatcag atcgttcaga
     5641 tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatcaac ttcgcgaccg
     5701 tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg gaagcgaatt
     5761 tccggggccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc catctcgata
     5821 atctgctcag aagtggtgcc acggacgatg gagtcgtcga ccagcaggac gtttttatcg
     5881 cggaactcgg cgcggttggc gttcagttta cggcgcacgg acttacgacg cagctgctgg
     5941 cccggcatga tgaaggtgcg gccaacatag cggtttttaa cgaagccctg gcggtacggc
     6001 ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttccgg gatcgggatc
     6061 accacgtcga tatccagatc ttcccattcg cgggcaattt tctcacccag tttcgtgccc
     6121 atattcacac gcgcgctgta aacggaaatt ttgtcgataa acgagtccgg gcgggcaaag
     6181 tatacatact caaacaggca cggattgctg accggattgt cagcacattg acgggtaaac
     6241 aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg caggaaatca
     6301 aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt acggttctcg
     6361 tcaatatcac gttttcccag taccagcgga cgaatcccgt ttggatcgcg gaaagcaacc
     6421 ataccgtggc cgataatcat cgccacacag gcatatgcgc cgcggattaa gcggtttgtg
     6481 gcagcaatgg cagcgaaaat attgtcggct tccagcgggt agtggcggaa gttgtccagc
     6541 tcgctagcga agatattaag cagaatttcc gagtcggaag tggtgttgat gtggcggcgt
     6601 ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc gttgtgggca
     6661 agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc gctggagctg
     6721 ccagccgtag ggtaacgcac atgaccaatg cccatattgc cctgcaaacg ctgcatatgg
     6781 cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa gcagttattg
     6841 gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac cgttaaggca
     6901 tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc gcacatacgt
     6961 ctttttcctc gttaagccac atctcagagc acttacgctc tgggcaagaa acttgacgag
     7021 ctttgcagat aatcaaaaaa ccatctgatg ataaaactga actgcgggat cagctgtgat
     7081 ttgctccagt cttcgctttt cgacaccccg gtaaaggagt cgagaaagaa gagaatggcg
     7141 gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg atcggtgcct
     7201 gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc accaacgatc
     7261 agggtagcga taaacagtac cgcgatggca attccatttc gaaccagttc gtcttcaaag
     7321 cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa agcacaaccc
     7381 catgtcacca gcgataacgc ttcacgaaca aagccgcgga tcaggctaac cagagaggaa
     7441 aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtccc acgattttac
     7501 gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg cgtagggatt
     7561 tccttcccac gcatcaataa aaatggcgct gaaaaaatat tcaacgccat cgacttttta
     7621 tgcctttgcg gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta tccgacctac
     7681 ggttctaaca ctgcccaagc ctgataagac gcgccagcgt cgcatcaggc aagactgtat
     7741 taattcggcg tatagcccat taccacgcca cttaagccag aaagttgctt caactcaccc
     7801 agcgaacctt tcagcttatc tttcgaggca tccggcccaa ccagaatacg ggtaatttta
     7861 ccctgcactg gcgtggatgg cgacgtataa acccgataac cggcaccgcg cagcttaccg
     7921 acaatctcat tcactttatc ggcatttttc agcgcaccca gttgcacaac ataggcttta
     7981 cccgttggcg ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg cgcaggtggt
     8041 gcttcaacct tcggtttagg cggctccacc ggtttcggct tcggtggggc gaccggtgca
     8101 ggttccggtt caaactcggt gttattggct gcaatagtgg ccggatcgag cgacggtgct
     8161 gcggcatcac ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt cggcaacgcc
     8221 tgggtggcgg ctggcatcat atcaggctca tcgcgatcgc ccgcttttgg aaccagcggg
     8281 atagccgcaa actcatcctg ataatgtttt ttctgcccgt ccagcagccc tggaagtaca
     8341 atcaccccca gcgccaccag cacgatcgtg cccactaacc gattctgaaa cttacttgcc
     8401 accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg accgtgtgga aagaaccaca
     8461 caccagcacg gtgtcttccg ctttagcgtc cgccattgcg gcatcccatg cctgcgcaac
     8521 gctatcaaat gatttgccgt tacccaaatg ctcaagcagt tgttctgccg tggcaccgcg
     8581 cggcccttcc agtggcgcac aataccagtc atcaaccacg cttttcaacc aggccagagt
     8641 tccggcaata tctttatcat gtagcatacc gataaccgcc agcacgcgcc cgtttttcgg
     8701 tagcgctttc atacgcccgg tgagatattc cgccgcatgt ggattatgcg cgacatcaaa
     8761 aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg cgctggcaat
     8821 cccgtcgcga atggcatttt cactgacttc cagcccgctg gcacgcagtg ccgccagcgc
     8881 tgttgcggca ttcggttgcg ggacaagcgg caacggcaga ttttccagcg tgccgtgagc
     8941 atcgctaaac gcccagtcat gatcggtgac ggaatagttc cactcaacgc cccgacgttg
     9001 taacagtgca cctttttcct gcgccacatc agcaatggta gaaggcattt ccggctcacc
     9061 gacaattgcc ggttttgcgc tgcgaaagat gcctgctttc tcgcgaccaa tactttcgcg
     9121 atctggcccc agccagtcgg tatgatccag cgcaatactg gttactaccg cgacatcggc
     9181 atcgacaata ttggttgcgt ccagacgacc gcccagccct acttccagaa tcaccacgtc
     9241 aagttgtgcc tgtttgaaca gccacaacgc cgacagcgta ccgtactcga aataggtcag
     9301 ggaaatatca ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg ccgattccgg
     9361 caattcctga ccctgcacac gtacgcgctc ggtataacgc accagatgcg gcgaactgta
     9421 gacgcccact ttgtaccctg ccgccatcag aatcgactcc agcgtacggc aggtggtgcc
     9481 tttgccattc gtacccgcaa cggtaaacac aaatggcgct ggtttcagaa cgccaagacg
     9541 agccgcgacc tggctcacgc gctcaaggcc gagatcgata gttttactgt gcaggttttc
     9601 cagataagaa agccacgaag ccagaggcga cgcggcttga ggagtgcgtt tgataatcat
     9661 ggtatccgct gattcgttac ggtgagaata gcaaaagggc agagccagtg gccctgccct
     9721 tatcagttat caggcctcag gttcctgatc cggtaccggg ggtaccacta cgccttcacg
     9781 cggcgcttca ggattcggcg ctggcagatt catcaacttc gccagaatgc tcgccagttt
     9841 caggcgcatt tccggacgac ggacgatcat gtcgatcgag cctttctcga tcaggaattc
     9901 actgcgctgg aatcctagcg gcagtttttc gcgaacggtc tgttcgataa cacgcggacc
     9961 ggcaaagcca attaacgctt tcggttcagc gatgttgaga tcgcccagca tggcgaaact
    10021 tgcagaaaca ccgcccatcg tcgggtcggt cagcacggag atgtacggca agccgcgctc
    10081 ctgcattttt gccagtgccg cagaggtttt cgccatctgc atcagcgaca tcagtgcttc
    10141 ctgcatacgt gcgccaccag aggcggagaa gcagatcagc gggcagttat cttccagcgc
    10201 ctgctcaacg gcacgcacga aacgtgcacc cacaacagac cccattgaac cgcccataaa
    10261 ggcgaactcg aatgccgcag cgacaaccgg cattccatac agagtgcctt tcatcaccac
    10321 cagcgcatct ttttcgccgg tttctttctg cgcagaagcc agacggtctt tatacttctt
    10381 ggagtcacga aacttcagca catctttcgg ctcaagctcg ctacccatct ccacaaggct
    10441 tccttcatct aacaggctat gcaggcgatt acgcgctgtc atacgcatgt gatggtcaca
    10501 cttcggacag acctcaagat tacgttccag ctcagcgcgg tataaaacct gaccgcagct
    10561 atcacactta gtccacaccc cttcaggaat gctcgccttg cgggtgggag taatgttgct
    10621 tttaattcgt tcaatccagc tcattaggga cctttctgtc tgaacctggt tcgatgccag
    10681 ttttatcttt ggggacgcat aatgccattt ttgcccccaa cagaccatga atgttgcaca
    10741 ttaaaacata acagcccgaa actttggata aaaaagtggt cgaaccgcgg agttactttt
    10801 tattttgcgg cgcgtgccgc agcgcgtttg tgacgaataa attcgaaaac cccaggcaat
    10861 attgaaacaa aaataatgcc aacaatcatc agcttaaggt tgctctgaat gaaggggagc
    10921 atgccgaaga aataacccgc gtaggtaaaa atcagtaccc acaacagtgc gccgatcacg
    10981 ttataagcgg cgaaatgacg gtacgacatg tggcccattc ccgcaacaaa cggcgcgaac
    11041 gttctgacga tcggtacaaa acgggcgaga ataatcgttt tgccgccatg tctctcgtaa
    11101 aactgatgag tcttatcaag ataactgcaa cgaaagattt tcgaatccgg gttactgaac
    11161 agtttgtcac caaataaccg tccaatggta tagttcactg catcaccgac aatagcggca
    11221 atcaacataa gcaccaccat catatggaca ttgagatcgt tagtttccag cgacgccaat
    11281 gcaccagcaa caaacagcag cgaatcacca ggcaggaatg gcgttaccac cagccccgtt
    11341 tcgcagaaca gaatcaaaaa cagaatggca taaacccaga cgccgtactc cgcgaccagc
    11401 tctgccagat gcacgtcaat gtgcaggata aaatcgatga ggaaataaat caggtccata
    11461 tttgcctatg ccttgtactc gtcatctttc aggctgtaac tacgttggct acagcctgaa
    11521 atgttccgag cattattctc gttagtccgc cagaaatagc gggcccattg gcggttttgg
    11581 aagatcatac cggtcagggt aatccaccgc gaccagatac agcccttccg cttttgccgt
    11641 tgctgccgcc agcgttctgt cctttgccgc cagcaactct gctatccagc tctccggctg
    11701 gttgtgggca ccgacttcca tcaggctgcc gacaatattc ctgaccatat gatgtacaaa
    11761 ggcattcgct ttgatatcta ccaccacata aggaccgtga cgcgtgacat taatatgcat
    11821 aacgttgcgc cacggggttc gggactggca ctgcaccgca cggaacgagg tgaaatcatt
    11881 ctcgcccagc aagcattgcg cagcccgatg catccgttca gcgtccagcg gttcgtaaaa
    11941 atgggttacc cctttactca gtaccgccgg gcgcagccga tgattgtaga tgatgtagcg
    12001 ataacggcga gccgtggcgc taaatcgggc atgaaaatca tcaggtacag ctttaaccca
    12061 acgcacggcg atgtcatcag gtaaattcgc atttacgccc aaggtccacg ccgcgtcttt
    12121 gcgctgcgcg gttgtttcga aatgcaccac ctgcccggta ccgtgtaccc ctgcgtcagt
    12181 acgcccggcg cagaagacgg tgatgggttc gttcgccacc tgggagagcg ccttttccag
    12241 cttctcctgc acactgcgga cttcattctg ccgttgccag ccgtaatact tactgccgtc
    12301 gtactcaatg cccagcgcaa ttttataaac tggcagttgt tgctggtcgg acattagtac
    12361 agatactcct gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc gccaaagcga
    12421 acgttatcgg ccaccgacca gaactggact tgctccggca taccgtagtc attacgcacg
    12481 cagccaacag aaagatgcgg agtacccgaa gcatccccta cctgagtcgg gaattcgttc
    12541 tcttcagaga gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc ttctgccgcc
    12601 agcggacgca gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac cggtgcctgg
    12661 acgacgcttg ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac ttcgtcaacg
    12721 atacgacgtt cttcacgcac gctaccttcg ctatccggca acaacggcag catattgaac
    12781 gccagttgac gaccgaagaa atcttcttcg tcaatcggaa tgccgttgag caatttcgca
    12841 ctctgccccg ctaacgcatc gaccgctttt ttgccctggg cggaggctga aatcaggctg
    12901 gtaacactga tacgcgacaa accgccctga tcgattaacg gtttcagtgc cgccagcagc
    12961 tggctggtca gactgtctgg tacggcgatg acattccggt tgcggtaatc ggtcagtaca
    13021 aacgggttta cttccggcac caccagcggt acgtcgggtt cgagagcaaa cagtccgctg
    13081 ctgtcgatca ccaggcaacc tgagttggtc gcttcttcaa cccaggcagc ggtagcttct
    13141 ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt cgaattcagc ggcatcctgc
    13201 acggtgattg ttttaccacc aaagcgcagt tgttcgcctg cgctttcgtt acgtgccagt
    13261 gcataaattt ccccaaccgg gaactgacgt tcagccagcg tttcaagcag ggcttcgccc
    13321 acagcgccag ttgcgcccag gacggcaatg ttccagcctt cagacatggt ggtttactcc
    13381 agaaatagca aagctccctg ccaatgttac agcagagagc atgaagaaga gattaacgtg
    13441 ccggatgatg aacggcgtta aaacccagtt tacacagcaa tgatgccgca ctggcgtcat
    13501 cacaaattac atacagagat gaccattcac ggcgctcaag atagtttttg cgcagtttat
    13561 cgaactcacc cggtatcccg gcgactttac gcagcggtgc gtcatcgcgg cgcacatcat
    13621 acaccaaatg caccagcctt ttcagcgtcg gttgatcgag cgggccatgc agcgtaatgc
    13681 gaccaaactc tggcgcgggc agtaatgtat ccagcgcaac gtgctgttca tgcccaataa
    13741 acttgctata agcttcaaac acttgcgtag taccgcgtgc tttaccttcc agggtataac
    13801 ctgcgatatg cggcgtgccg atatccactt ttgtcagcaa ctccacgtta agttccggtt
    13861 cgccttccca gacatccagc actacgctta acttctggcc ttcattcagg caggtcagca
    13921 acgcagtatt atcgacgact gcgccacggc aggcgttaat cagaatcgct cccggcttca
    13981 ggctacggat cagtttttca tccgccagat gcagcgtttt gtacggccca tctttgaaga
    14041 gtggcgtatg gaaagtcaga atatccgcat gctgaactaa ttcatccagc gagcggaaat
    14101 caccctcatc cccacggtca gcgcgaggcg gatcgcaaag caatgtttta atccctaacg
    14161 cttccagtcg cgcctgcaat cgacgtccaa cgttaccaac gcccacgatc cccaccgtac
    14221 ggtcgtgcag tgaaaatcca tcgcgttcgg caagcatcag tagtgaggag aaaacatatt
    14281 ccaccaccgc aatcgcatta cagccaggtg cagcggaaaa accaattccc gcctgcttca
    14341 accatgcttc atcgacatgg tcggtccccg cagtggcagt gccaacaaat ttaatgggtt
    14401 ttcctgccag caaagattca ttcactttcg tgaccgaacg caccatcagc gcatccgcgt
    14461 ctgccagttg agcgacggcg attggacgcc cgggaaccgc ggtcacctca cccaaacggc
    14521 taaataagtc gcgggcataa ggcatatttt catcaacaag gattttcacg tttgtgttac
    14581 ctgtatgaga cgagagttaa ccggacaagt gtgccataat ctcgcggcca ggcatacttg
    14641 cgaagatttc aggtataagg atacgtaatg atacaaccta tttccggccc tcctcctggg
    14701 caaccaccag gtcagggaga taacctgccg tctggcgcgg gcaatcagcc tttatccagt
    14761 cagcaacgta cttcgctgga aagcttaatg acgaaagtga cctcactgac gcaacagcaa
    14821 agagcagaac tgtgggcggg tatcaggcac gatattggtc tgtcgggaga ttcaccgctg
    14881 ctttcgcgtc acttccctgc cgctgagcat aatctggcgc aacgtctgct ggccgcgcaa
    14941 aaaagccatt ctgcccgcca gcttttagcg caattagggg agtatttacg tctggggaat
    15001 aatcgtcagg cggtcacgga ttatatccgt cataactttg gtcagacgcc gctgaatcag
    15061 ctctcaccgg agcaattaaa aaccattctc accctgttgc aggaagggaa gatggttatt
    15121 ccgcaaccgc agcagcgcga ggcgaccgac cgtcctttat taccggcgga gcacaatgcg
    15181 ctaaaacagc tggtgaccaa acttgcggcg gcaacggggg aacccagcaa acagatctgg
    15241 caatcgatgc tggaactttc cggggtgaaa gatggcgagt taattccagc gaaactgttt
    15301 aaccatctgg tgacctggct acaggcgcgt cagacgctaa gccagcaaaa tacgccgacg
    15361 ctggaatcac tacagatggc gctaaaacaa cctttagatg ccagtgaact ggcggcgtta
    15421 tcggcatata tccagcaaaa atatggcctt tctgcgcaat catcgctttc ttctgcccag
    15481 gccgaggata ttcttaatca gctttatcaa cggcgggtta aagggattga tccgcgtgat
    15541 atgcaaccgc tgcttaatcc ttttccaccg atgatggaca cgttgcaaaa tatggcaacg
    15601 cgtcccgcgc tgtggatact gttagtcgcg attatcctga tgctggtctg gctggttcgt
    15661 taaccccgac gaaatgacag tatcgtgaca ataataccca gcaccgcaga gatcgccccg
    15721 gcaagaaata ccgaagagta accaaacgtg gtcgccagca ttcccgccag cggcccggag
    15781 acgccgaggg cgatatcctg aaacgcggcg taaccgccca gtgcggtgcc gcgaacttgt
    15841 gaggggacgc gtttaaccac ctccacgccc agcgcaggaa agataagcga acatccggct
    15901 ccggttaacg ccgcgcccgc taatgcgacc catgcacctg gggcttgcca gagcagcaac
    15961 aagcccaccg tttctacaag cagagagaca atcgccactt tcacgccgcc aaaacggtcc
    16021 ggcatccagc caaacatgac gcgcatcacg acaaatgcgc cgccaaacgc ggtaagagta
    16081 aagcccgcca tcgcccatcc tttgctggca aagtagagcg aaacgaaagt cccgataacc
    16141 gcaaaaccaa cgccttgtag tgccagccct aaccctggtt tccagataag cccgacaacg
    16201 ctccacagcg atggacgttc tcccgccagg gccggtactt tgcgcactgt gccgttacag
    16261 gcccacgcca gtaagggtaa taccattgtg gtgatcgcca gtgcggcaaa accgtaatgg
    16321 ctatgaatca acaggccaag cggcgcacca acagcgaggg caccgtaaat cgccattccg
    16381 ttccatgaca tcactttgcc agagtgtttt gcccctacga tgcctaatcc ccaggtcaga
    16441 gcgcctgtca gtaactggct ttcaccaaag ccaagaatca aacgcccgac gaccaacagg
    16501 gcaaatttca acggtgcgga gacaggcaaa atcgccgcca gcagcaacgc gccgccagcc
    16561 agaccacaag ctaacatccc ctgaagcgcc gaacgttttg caccatattg atcggccagt
    16621 cgcccggcat agccacgcgt cagcaccgta gccagaaact gaatcccgac ggcaatcccg
    16681 accatggtat tgccatagcc cagatcatga tgaacaaaca gcgggataac cggcaacggc
    16741 agccctacgg tcatgtaggt gagaaaaacc gcaaaagcga tgcggaagag cgaaaaattg
    16801 gcagaagatc gtgtttcggt ttggcttaca gcagtcatgc attactccag aatgcagcgc
    16861 aaggcgagga gtatccccgt ctcatctctc tggtttcagg gttacagtgc gttggcagga
    16921 tttaacgcgt acgtcttttc agaaggaaat cgacaaagcg ggaagtttgc ctggaactgg
    16981 cggcgattgt caatgatgtg aaaaagggaa ccatcaggtt cccttttgcg ttagtgccgg
    17041 aggccgcatt ggcgcgtaac gtcggatgcg acgctggcgc gtcttatccg acctactccg
    17101 actactccga ctactccgac tactccgacc tactgcgaat taatctttca gcttgcgcat
    17161 taccagcgtg gcgttggtgc cgccgaagcc gaagctgtta gacataacgg tggtcagttc
    17221 gcgatcggtc gtttcggtca cgatgttcag acctgccgcc tgctcgtcca gctcttcaat
    17281 gttgatgctc ggggcgataa agccgtgttc cagcatcagc agagagtaga tagcttcctg
    17341 tacgccagca gcgcccagag agtgaccggt catggctttg gttgcagaaa tcgccgggct
    17401 cttatcgccg aacacttcac ggatagctgc cagttctttc acgtcgccaa ccggagtcga
    17461 agtaccatga gagttcaggt aatcgattgg ggtatcaacg ccgtgcatcg ccatcttcat
    17521 gcagcgtact gcgccttcgc cagacggagc aaccatgtct gcaccatcag aggttgcgcc
    17581 gtagccaacg atttcagcat agatgtgagc accacgcgcc agcgcgtgtt ccagctcttc
    17641 aaccactacc ataccgccgc cgccagcgat aacgaaaccg tcacggtgag cgtcgtaagt
    17701 acgggaggct ttttccgggg tgtcgttgta tttagtagac agcgcaccca tcgcgtcgaa
    17761 ttcgcaggcc atttcccagc acagctcttc gccgccgcca gcaaacacga tgtcctgttt
    17821 gcccagttgg atctgctcta ctgcgttacc gatacagtgt gcggaagtcg cacacgcgga
    17881 gctgatggag tagttaacgc catgaatttt aaacggcgtg gcgaggcagg cagaaacgcc
    17941 ggatgccatc gctttggtga ccacatacgg gccaaccgct ttcaggccgc gcgggccgcg
    18001 cattgcgtca gcgccgaata cctggaaacg cggggaaccg ccgccagaac ctgcaatcag
    18061 gccaacgcgc gggttattct ggtaagcttc cggagaaagg cccgcatctg cgattgcctg
    18121 ctccatagaa aggaatgcat aaatggatgc gtcgctcata aagcgcacaa ctttgcggtc
    18181 aatgaggcca gtggtatcca gttttacgtt gccccagacg tggctacgca tgccggaatc
    18241 cttaagctcc tgagagaaag tgatccctga acgtccttca cgcagagatg ccaggacttc
    18301 ctgctggtta ttaccgatgc tggaaacaat gcccaggcca gtaatcactg cacgtttcat
    18361 tcaatacctc tgtaagtcgc acatagagta agtttcgaat gcacaatagc gtacacttgt
    18421 acgccgaaca agtccgatca gccatttaat agagaaattt gcgcagcctt acacacatcg
    18481 ctaagatcga gccaccgcct gtaagacgag taacttacgt gaaacactac tccatacaac
    18541 ctgccaacct cgaatttaat gctgagggta cacctgtttc ccgagatttt gacgatgtct
    18601 atttttccaa cgataacggg ctggaagaga cgcgttatgt ttttctggga ggcaaccaat
    18661 tagaggtacg ctttcctgag catccacatc ctctgtttgt ggtagcagag agcggcttcg
    18721 gcaccggatt aaacttcctg acgctatggc aggcatttga tcagtttcgc gaagcgcatc
    18781 cgcaagcgca attacaacgc ttacatttca ttagttttga gaaatttccc ctcacccgtg
    18841 cggatttagc cttagcgcat caacactggc cggaactggc tccgtgggca gaacaacttc
    18901 aggcgcagtg gccaatgccc ttgcccggtt gccatcgttt attgctcgat gaaggccgcg
    18961 tgacgctgga tttatggttt ggcgatatta acgaactgac cagccaacag gacgattcgc
    19021 taaatcaaaa agtagatgcc tggtttctgg acggctttgc gccagcgaaa aacccggata
    19081 tgtggacgca aaatctgttt aacgccatgg caaggttggc gcgtccgggc ggcacgctgg
    19141 cgacatttac gtctgccggt tttgtccgcc gcggtttgca ggacgccgga ttcacgatgc
    19201 aaaaacgtaa gggctttggg cgcaaacggg aaatgctttg cggggtgatg gaacagacat
    19261 taccgctccc ctgctccgcg ccgtggttta accgcacggg cagcagcaaa cgggaagcgg
    19321 cgattatcgg cggtggtatt gcctgcgcgt tgttgtcgct ggcgctatta cggcgcggct
    19381 ggcaggtaac gctttattgc gcggatgagg cccccgcact gggtgcttcc ggcaatcgcc
    19441 agggggcgct gtatccgtta ttaagcaaac acgatgaggc gctaaaccgc tttttctcta
    19501 atgcgtttac ttttgctcgt cggttttacg accaattacc cgttaaattt gatcatgact
    19561 ggtgcggcgt cacgcagtta ggctgggatg agaaaagcca gcataaaatc gcacagatgt
    19621 tgtcaatgga tttacccgca gaactggctg tagccgttga ggcaaatgcg gttgaacaaa
    19681 ttacgggcgt tgcgacaaat tgcagcggca ttacttatcc gcaaggtggt tggctgtgcc
    19741 cagcagaact gacccgtaat gtgctggaac tggcgcaaca gcagggtttg cagatttatt
    19801 atcaatatca gttacagaat ttatcccgta aggatgactg ttggttgttg aattttgcag
    19861 gagatcagca agcaacacac agcgtagtgg tactggcgaa cgggcatcaa atcagccgat
    19921 tcagccaaac gtcgactctc ccggtgtatt cggttgccgg gcaggtcagc catattccga
    19981 caacgccgga attggcagag ctgaagcagg tgctgtgcta tgacggttat ctcacgccac
    20041 aaaatccggc gaatcaacat cattgtattg gtgccagtta tcatcgcggc agcgaagata
    20101 cggcgtacag tgaggacgat cagcagcaga atcgccagcg gttgattgat tgtttcccgc
    20161 aggcacagtg ggcaaaagag gttgatgtca gtgataaaga ggcgcgctgc ggtgtgcgtt
    20221 gtgccacccg cgatcatctg ccaatggtag gcaatgttcc cgattatgag gcaacactcg
    20281 tggaatatgc gtcgttggcg gagcagaaag atgaggcggt aagcgcgccg gtttttgacg
    20341 atctctttat gtttgcggct ttaggttctc gcggtttgtg ttctgccccg ctgtgtgccg
    20401 agattctggc ggcgcagatg agcgacgaac cgattccgat ggatgccagt acgctggcgg
    20461 cgttaaaccc gaatcggtta tgggtgcgga aattgttgaa gggtaaagcg gttaaggcgg
    20521 ggtaatctgc tctcgcagtg tttgccggat gcggcgtgaa cgccttatcc gacctacgtg
    20581 tgacgtgtag gcctgataag acgcgataag cgtcgcatca ggcatggtgc tccagacgcc
    20641 gcaacttact gctgtgacgc ctgctgaaac aggttttccc acatatcggt caccagcgcc
    20701 tggtcacgcg gcgacaattc accggcacca atggcttttt ccagactctg gctaacggtc
    20761 gtatgcaccg cctgagcgga gtggtcatca ccactttcca gttctgcgat ggctaatgtc
    20821 aggtggccac gcaaataccc actggcaaac aactcatcat cactggcatg gtcaatcatg
    20881 ccgtcgatta atgccagaat gcgtgattca aactccgcga tcatcttctt tcctcattgt
    20941 aaaacgtggc accggcttca gttgagcgct tccggccacg ggaactgttc cgccgtaagt
    21001 tccggcgtgt gataataatt ttgtagtgct ttaatgaagc gtgccggacg ctcgggaatg
    21061 ccatttgtca gataatccat cacctgcgca tgtacccgcc gttgaaacac cacacgatcc
    21121 ggttcgaagt cgccttccag attgtcgcaa ctgacgttaa acggatatcc cgccgccaca
    21181 cagaacaacc agtcgagtgc ctgcggcttc acttcaacat cttcaaactg gctttgcgtt
    21241 tgggcatcac gcccatccgg gcaataccag tagccgaaat caaccagctc acggcgcgct
    21301 tttccggcaa tacaccagtg cgaaatctca tgaattgcgc tggcataaaa gccgtgggca
    21361 aagacaattc ggttatacgg tacttccgca tcagcaggaa gatagatcgg ttcgtcgtcg
    21421 cctttaatca gacgggtatt aaattcatcg gcaaagcagc tattaaaaat ttcaatcaac
    21481 tgctcatagt ggtgtgtact gttcattagt tcatccccaa ccagtggagg atctcctgtc
    21541 cgtggctatc gtaaagtaat ttggcactca tcaccgccga gacgataacg atcatcgggc
    21601 ggatcagttt ttgtcctttg ctcagcacca gtcttgaacc catgcgcgcg ccgaggaact
    21661 gccccaccag catgacaaag cccgtcgccc aaatcacttt gccgccgaga ataaacagca
    21721 gcagaccgcc aatgtttgac gttgcgttga gtaatttggc gtgagccgtg gctttggcga
    21781 ggttgaatcc gcacagcgta acgaaggcca gcgcgtaaaa cgatccggca gccgggccaa
    21841 agaatccgtc ataaaaaccg acgcagccac cggcaattaa cgcgaagggt aaaccgtaca
    21901 tccggcgctg gcgatcttct tcacccagct ttggcatcag caagaaatag aggccgatac
    21961 aaattaccag aatgggcaaa atctgccgta agacatcagc ctgaacgtat tgcaccagca
    22021 gtgcgccgct cattgagccg gcaaaggtca tggcgatatt gagtttctga tcgcttaaac
    22081 taaccacttt gcggcgaata aagtagatag tggcggaaat agagccgccg caggcttgca
    22141 gtttattggt tgccagcgcg ttagcgggag acattcccgc agccatcaat gccggaatgg
    22201 tgagtaaccc accgccaccg gcaatcgagt cgataaatcc cgccagcatg gcgacaaaaa
    22261 agagaactcc cagcaacagc ggggaaacca taaacaggct attaaacgtt tccattagat
    22321 cacgtgctca tccagtagcg cctggcagga aggcggcaac ggaggcggtg tcttcttctc
    22381 aggctttgtt gttcctggtt ttggaggtgc aaaccagctt tgcagttctg ccccgcaacc
    22441 atcgcctggt ggcggtaaag gttgatcttc acactccaga ctatcggcag ggcaacgtaa
    22501 tcgtacatgc atatgcgcgc gatgctggaa ccagggtcgc actttgcgca accagtcgcg
    22561 atcggtgccc gcatcaaggc aaagttgttg tttaatcgcc ggattaacaa aaatgcgcgt
    22621 gacgtctttg tcctgggcgg cgagtttgat caagctgaaa atttctggct tccacagcgt
    22681 ggagacaacg tgtttaccgt cgcgggaaac taagtccagt gcttgcgggc gcaagagctg
    22741 cgcggaggtc cagcgagttt tcggcagttg cagaaagata tcgacatcca gtccggtctg
    22801 gtggctggca tgaccgccgt tgaaacgccc accagcgggc atccccatat cgccaatcag
    22861 caccgtaccc atgcccagat tgctcacctg gctactcaga cgctggataa acatcaccag
    22921 atccgggtga ccgaaatagc gacgctgatc ggtacgcatg acctgataat gttcggactg
    22981 tatcggcaac gtgtcagcgc cgacaataca gccattagaa aaactgccta tcgattgtgc
    23041 gctacccggc acaggttggg ttattttttg ccacggcgtc gctgccaagc tagcgctact
    23101 ggcaagcaga gccagcagcg caatcgcggt tttgttcatt ttttaccagc gtggaatatc
    23161 agtcttcaca tcggcatttt gcgcccgctg ccgtaacagg tgatccatta aaacgatcgc
    23221 cagcatcgct tcagcgatcg gcactgcacg gatcccgaca cagggatcgt gacgaccttt
    23281 ggtgatcatc tcaacttctt cgccaaagcg gttaatagtg cgccccggca cggtaatgct
    23341 ggaggtcggt ttcagcgcca tatgagcaat gatttgctgc ccgctgctga taccgccgag
    23401 aatgccgccc gcatggttgc tctggaaacc gtctttggtg atttcgtcgc ggttctggct
    23461 gccgcgcagc gccactacgt caaagccgtc gccaatttcc acgcctttca ccgcgttgat
    23521 gctcatcagc gcatgggcga tgtcggcatc caggcggtca aataccggct cgccaagtcc
    23581 ggcggggacg ccactggcaa caacggtgac tttcgcgccg atggagtcgc cctctttttt
    23641 cagcgcgcgc atcagttcat ctaacgcgtc gattttgtcc ggatccgggc agaagaacgg
    23701 attttgttca accagcgacc agtctttgat ttccagcgga atgtcgccca tctgggtcaa
    23761 gcagccgcgg atttcaatac caaatttctc ggcgagatat tttttggcaa tcgcccctgc
    23821 cgccacgcgc atggcggttt cgcgggcgga agaacgtcca ccgccgcgat aatcgcgcag
    23881 accgtatttt tgttcgtagg tgtaatcagc atggcctggg cggaaaacgt ctttaattgc
    23941 gctgtaatcc tgagagcgct ggtcagtgtt ttcgatcaac aagccaatgc tggtgccagt
    24001 agtaacgcct tcaaaaacac cagagagaat tttgacctga tccggctcgc ggcgctgggt
    24061 ggtatagcgc gatgtcccag ggcgacgacg gtcgaggtca tgttgcaggt ccgcttccgt
    24121 cagcagaatg cctggcggaa caccatcgac gatgcagccg agcgccagcc catgcgattc
    24181 gccgaaggtg gttacgcgaa agagttgtcc aattgtgttt ccagccatca cggctccgtt
    24241 attgttgtgt ttgcgtgttt acttaatctt tataaatcgc gaaatattca cgggccgcca
    24301 gcagctgctc tttggtgagc ataaacacac catcgccgcc gttatcaaac tccagccagg
    24361 tgaacggaac atccggatat tgttccataa gatgtaccat gctgttgccg acttcacaaa
    24421 tcaacacgcc atcatcagca aggtaatctg ccgcgtttcc aagaatgcga cgcgtcagtt
    24481 taaggccgtc ggtgccagat gccaggccca gttccggctc gtgacggtat tcgtttggca
    24541 ggtcggacat atcttccgcg tcgacatacg gcgggttagt gacaatcaga tcgtactgca
    24601 ctttcggcaa gtcgcggaac agatcggaac gaatcggaat gacgttgtgg atcagaccgt
    24661 gttcttcgat gttctgttca gcaaccgcca gcgcgtcggg agagatatcc accgcgtcga
    24721 cttctgcatc cgggaaggca taagcacagg caatggcgat gcagccgcta ccggtacaca
    24781 tatctaaaat atgctgcggt tgtttgctga tgagtccggc aaatttattg ttgatcagtt
    24841 caccaatcgg cgagcgcggc accagcacgc gttcatcgac gtaaaattca tgaccgcaga
    24901 accaggcttt gttggtcagg taagccaccg gaatgcgctc gttgacgcgg cggatcacgc
    24961 gttcaacaat acgatgtttt tcgctggagg tcagacgcgc ggtgcgcata tcttccggga
    25021 tatccagcgg caggtagagc gatggtaaca ccagctgtac ggcttcatcc cacgggttat
    25081 cggtgccgtg accgtaccag atatttgccg cgctgaagcg gctcactgac cagcgcaaca
    25141 tgtcctgaat ggtttgcagc tcatttactg cttcatcaac gaaaatttta tccacgtatt
    25201 cctccagggc atgatcgcaa taatttcggc ggctagtttg ccatgaagat gacgataaat
    25261 cagcattcac gcgcggtgag tgaggaaaaa tacgtttaaa acgatcgatt gtgctacgag
    25321 tcgggtaaac tgtaggaaaa ttagaaatag agacagataa atgaaaaaga aaacaacact
    25381 tagcgaggag gaccaggctc tgtttcgcca gttgatggcg gggactcgca agattaagca
    25441 ggacacgatt gtccaccgac cgcagcgtaa aaaaatcagc gaagtgccgg tgaaacgctt
    25501 gatccaggag caggctgatg ccagccatta tttctccgat gagtttcagc cgttattaaa
    25561 taccgaaggt ccggtgaaat atgttcgccc ggatgtcagc catttcgagg cgaagaaact
    25621 gcgccgcggc gattattcac cggaattgtt tttggattta cacggtctga cgcagctgca
    25681 ggccaagcag gaactgggcg cgttgattgc cgcctgtcgc cgcgaacatg tgttttgtgc
    25741 ctgcgtgatg catgggcacg ggaagcatat tttgaagcaa caaacaccgc tgtggctggc
    25801 acaacatccg catgtgatgg catttcacca ggcaccgaaa gagtacggcg gtgatgcggc
    25861 tttgttggtg ttgattgaag tggaagagtg gttgccgccg gagttgccct gaggagttga
    25921 gcgtgttgtg ccaggagcgc ggaatggcag gggcgaatga ccctgccagg tgagaaaagg
    25981 tgcgctaata catattcagt atgtaagtga atcggcaggt agaatctatg ggctgtcgag
    26041 atgtcgtgtc cacgcgtagc ttcatatcat gcagttgcct ggacgtaatc ggttgcggct
    26101 caggcgtcac cgtcactttg gcattatcac agccggaagt cgagaaggtc atctgctgtg
    26161 cgataaaacg gcagtagaca ccacgctcat caaaattatt tccgttacac agtttgccat
    26221 tttccatcag gaaagtgggc agtgtttgct caataacacc acctgttgac tgcaacctga
    26281 cgactttgtt atgtacaaca taattttcat tccaggaaac atcatacggt gccaggggca
    26341 ccgccggaca tgaagaactc accttcgttg ggccaatata gtcaggaaaa tcatcatcat
    26401 acgttttagc gaagggatct ctactcttaa caaacgacga cacaatccac ggacaaccat
    26461 gtaaattccc atcgtaataa acaaacatct ttaattcatt acatgtccat cccgttttgg
    26521 tctggctatc atactgctct aatccaggac aggctgacat ggataccctg ctgtccgaaa
    26581 ttttcgacat agatggttga gtagaactat cgccagtcca cgaaactaaa tccggctgtg
    26641 aattgggcat gaatgtagcc ttaaatatag cgtaattaac tgaatctggc gtcgctataa
    26701 tcagatgaaa ctcaaactc
//
LOCUS       DAJTOR010000041        25806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTOR010000041 DAJTOR010000000
VERSION     DAJTOR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.041"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(82..2301)
                     /locus_tag="OO249_001527"
     CDS             complement(82..2301)
                     /locus_tag="OO249_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5635872.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSSEEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVLHPAYGNVRIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(2412..3824)
                     /gene="ftsP"
                     /locus_tag="OO249_001528"
     CDS             complement(2412..3824)
                     /gene="ftsP"
                     /locus_tag="OO249_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5635873.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDSRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAASPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3899..4636)
                     /gene="plsC"
                     /locus_tag="OO249_001529"
                     /gene_synonym="parF"
     CDS             complement(3899..4636)
                     /gene="plsC"
                     /locus_tag="OO249_001529"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5635874.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4869..7127)
                     /gene="parC"
                     /locus_tag="OO249_001530"
     CDS             complement(4869..7127)
                     /gene="parC"
                     /locus_tag="OO249_001530"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5635875.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSLMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(7673..8155)
                     /locus_tag="OO249_001531"
     CDS             complement(7673..8155)
                     /locus_tag="OO249_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5635876.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(8208..8600)
                     /gene="ygiW"
                     /locus_tag="OO249_001532"
     CDS             complement(8208..8600)
                     /gene="ygiW"
                     /locus_tag="OO249_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5635877.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8752..9411
                     /gene="qseB"
                     /locus_tag="OO249_001533"
     CDS             8752..9411
                     /gene="qseB"
                     /locus_tag="OO249_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5635878.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLASEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9408..10757
                     /gene="qseC"
                     /locus_tag="OO249_001534"
     CDS             9408..10757
                     /gene="qseC"
                     /locus_tag="OO249_001534"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5635879.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPSGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            10867..11448
                     /gene="mdaB"
                     /locus_tag="OO249_001535"
     CDS             10867..11448
                     /gene="mdaB"
                     /locus_tag="OO249_001535"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5635880.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11479..11793
                     /locus_tag="OO249_001536"
     CDS             11479..11793
                     /locus_tag="OO249_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5635881.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11841..13733)
                     /gene="parE"
                     /locus_tag="OO249_001537"
     CDS             complement(11841..13733)
                     /gene="parE"
                     /locus_tag="OO249_001537"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5635882.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13762..14343)
                     /gene="yqiA"
                     /locus_tag="OO249_001538"
     CDS             complement(13762..14343)
                     /gene="yqiA"
                     /locus_tag="OO249_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5635883.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(14343..15170)
                     /gene="cpdA"
                     /locus_tag="OO249_001539"
     CDS             complement(14343..15170)
                     /gene="cpdA"
                     /locus_tag="OO249_001539"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5635884.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(15195..15617)
                     /locus_tag="OO249_001540"
     CDS             complement(15195..15617)
                     /locus_tag="OO249_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5635885.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(15618..16247)
                     /gene="nudF"
                     /locus_tag="OO249_001541"
     CDS             complement(15618..16247)
                     /gene="nudF"
                     /locus_tag="OO249_001541"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5635886.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHSLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            16452..17933
                     /gene="tolC"
                     /locus_tag="OO249_001542"
     CDS             16452..17933
                     /gene="tolC"
                     /locus_tag="OO249_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5635887.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            18081..18752
                     /locus_tag="OO249_001543"
     CDS             18081..18752
                     /locus_tag="OO249_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5635888.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            18758..19918
                     /locus_tag="OO249_001544"
     CDS             18758..19918
                     /locus_tag="OO249_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5635889.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLPLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(19956..20744)
                     /gene="ygiD"
                     /locus_tag="OO249_001545"
     CDS             complement(19956..20744)
                     /gene="ygiD"
                     /locus_tag="OO249_001545"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5635890.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEARGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            20887..21648
                     /gene="zupT"
                     /locus_tag="OO249_001546"
     CDS             20887..21648
                     /gene="zupT"
                     /locus_tag="OO249_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5635891.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGISPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(21706..21876)
                     /locus_tag="OO249_001547"
     CDS             complement(21706..21876)
                     /locus_tag="OO249_001547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635892.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(22136..22789)
                     /gene="ribB"
                     /locus_tag="OO249_001548"
     CDS             complement(22136..22789)
                     /gene="ribB"
                     /locus_tag="OO249_001548"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5635893.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(22893..23041)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            23163..23453
                     /gene="ubiK"
                     /locus_tag="OO249_001549"
     CDS             23163..23453
                     /gene="ubiK"
                     /locus_tag="OO249_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5635894.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            23737..24288
                     /locus_tag="OO249_001550"
     CDS             23737..24288
                     /locus_tag="OO249_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR5635895.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILNSQSGEVTLGTPVVITFNNTNSYQELNFKVRM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            24348..>25806
                     /locus_tag="OO249_001551"
     CDS             24348..>25806
                     /locus_tag="OO249_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5635896.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNAIINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLHGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDTQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTN"
BASE COUNT         6461 a         6799 c         6373 g         6173 t
ORIGIN      
        1 tcggcatata aataaatgcc taatgagata gattctgatg taatatcccc ggtagcaatt
       61 ctgctaccgg gaacagcgaa tttaacgtcc aaccgccgct ttaggccgtt tcttcgcacc
      121 agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag gcgtctgacg
      181 ctgggtcgcc atcggcgtat gtttcgtcaa cgccggacgg gtattgcggt tctggcgacg
      241 agcttcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac ggctaccaat
      301 cagatgcttt ttacccatcg cttccagcgc ctggcggatt aacggccagt ttgccggatc
      361 gtggtaacgc aacaacgctt tatgcaaacg acgctgcttg tcgcccttcg gtacgaagac
      421 gtcttcactc ttataaccaa tcttcgccag cggttttttc ccggtgtaat acatggtggt
      481 tgagttcgcc agcggcgatg ggtagaagtt ctgtacctgg tcgagacgga aacgatgctt
      541 tttcagccac agcgccagat tcaccatatc ttcatcacgc gtaccggggt gcgcggagat
      601 gaaatagggg atcagatact gctctttacc tgcctgtttt gagtaagtat cgaacagctc
      661 tttaaagcgg tcatagctgc ccatgcccgg cttcatcatc ttcgataacg gcccttcttc
      721 ggtatgttcc ggggcaatct tcagataacc gccgacgtga tgggtcgcca gttctttgat
      781 atagcgcgga tcttccacgg cgatgtcata acgcacacca gaggcaatca ggatcttttt
      841 aatgcctttc agatcacgcg cacggcgata gaggttgatc gttggttcgt ggttcgtgtc
      901 catgtgcgga caaatatccg gataaacgca cgacaaacgg cggcaggttt gctcagcgcg
      961 tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat cggaaattac
     1021 gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga tcgaatcttc
     1081 ggaacggctc tgaataatgc gcccttcgtg ctcggtgata gaacagaaag agcagccgcc
     1141 aaagcagcca cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg gaatacggac
     1201 attgccatag gccggatgta gcacgcgctt gtatggcagc gcaaaaacgc tgtccatctc
     1261 ttcggtagaa agcggaatag caggcgggtt gatccacaca tagcggtcgc cgtgtttttg
     1321 catcaatgcg cgggcacagc ctgggttggt ttcgtggtgc agaatacgcg aagcatgggc
     1381 gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaacacgt aggttttttc
     1441 ccacggtttc gggcgcggtg gctgcacggt tacggctttg gcttcctgct ttttcggtgc
     1501 caccggtttg ttatccgcgc acggcaaatc ttcaccatac ggatgcggga ttgggtcgat
     1561 ttttccaggg gtatcaagac gggtggaatc cacgccgctc cagccaggca gggcctcttt
     1621 cacgataatc gcggtattac gcacatcgcg gatttcacta atcggctcgc ccatcgccag
     1681 acggtgcgcc acctccacca gcggacgctc accgttacca aacatcagca tgtcggcttt
     1741 cgaatccacc agcacggaac ggcgcacggt atcggaccag taatcataat gtgcggtacg
     1801 acgcagacta gcctcaatac cgccgaggat caccggtaca tctttccacg cctctttaca
     1861 acgctgggta taaaccagtg tggcgcgatc cgggcgctta cccgcgacgt tatccggcgt
     1921 gtaggcatcg tcatgacgta aacggcgatc ggcggtataa cggttgatca tcgaatccat
     1981 gttgccagca gtaacaccga aaaacagatt cggtttaccc agacgcataa agtcgtcttt
     2041 gctgctccag tccggctggg cgatgatccc gacgcgaaag ccctgcgctt ccagcatacg
     2101 accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag taaccaaaat
     2161 gatgtcgcag ctatcccagc caagttgatc catctcttca ctagacatcg gcaaaaacgg
     2221 tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc gatccggttg
     2281 gatcagggag atagagctca ttttgcttcc agaaatgata aaaaaataat caaaggccgg
     2341 ggattataag ccggaacgaa agagaaatcg aaaagtattc catactcgcc ctcctcgggc
     2401 gagtatgaag attacggtac cggattgact aacagttgcc caatcgagcc acggtccgcc
     2461 atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg ctgaccgaaa
     2521 taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc tctgtcttcc
     2581 gggaacggca tcgcaccatt cacgttacgg atctggaaca tcacgccttc aatatggaac
     2641 gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg cgcggtgaca
     2701 tcaatacggt tgacgtccca cagttgtcca ttaatacccg ggtcatcacc cagactgata
     2761 tcgcgactgc gaatcggcga agcggccatg atttcagttg gcagcaagcg catcggaaga
     2821 ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccag ggtagaaacc
     2881 agaatgctgg atggctcaaa gaagccacga atacgatcaa caatgctcgc cgcttcgcca
     2941 caggtgatcg acacttcatc accgttgctc atatccacca gaatctcgcg gcgctcgccc
     3001 ggtgccaacg aaagttgctt caccgataca ggagcaggca ggaatccctg atcgccagaa
     3061 atcacatgta acgggcgact atcgctcatc tgtagttgat agcgacgaga gttcgacgcg
     3121 ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct ttgtacaccg
     3181 ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta ttctggcgta
     3241 ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac accataatgg
     3301 ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc ggcaaggccg
     3361 ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag agtagctgcg
     3421 ttctggcgaa tgggcagtac gggtgcccag tcagcgtttg gcgacatcat ccgtgccgga
     3481 ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac attttctgtc
     3541 aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat agtcggcccc
     3601 aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa tgaccagtgc
     3661 gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagtag cggcggaacg
     3721 ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc ggcacaaagt
     3781 gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc tccatgtaaa
     3841 actggctaaa gtaataccgt gcaggaaagt gttccccgca aaaatacaga ttgccgactt
     3901 aaacttttcc ggcggcttcg cgttctgcga cttctttatc gagctcggcg attttttgtt
     3961 ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca tactgactga
     4021 cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga ttaagattaa
     4081 tcttattcga agttgtagag acgcacacgg gaataatcgg gacgcccgcc gcaattgccg
     4141 cgtgaaatgc tccagtcttg aacggtagca ggccgcgacc acggctgcgg gttccttccg
     4201 ggaacatcca gatggaaata cggcgttttt tgaaatgatt cacgacttcc gcaatggtgc
     4261 cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac cagtacaact
     4321 gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc ggcggttgca
     4381 caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata tagatagcat
     4441 tgccgtagct ttcagcatct gcaggtttgc ggcactcaac tttcaggccg aacagcggcg
     4501 caagacggcc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc gggctgaaaa
     4561 ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta ataataagac
     4621 gaaagatata tagcataaca acctctgaag gtctgagaga ctggcattgt acgtcacctg
     4681 aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt taatcgcaac
     4741 gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg aatacgctgc
     4801 cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa caggccctcc
     4861 ccggcatatt actcttcgct atcaccgctg ctggcacggc gaggagagtc gatctcaaca
     4921 cgatcgatac gctgcaaacc gcgcatcaac gtaccgcggc gtccacgttc gccagtgact
     4981 ttctgtaact cttccgggcg cagtttaatt ttgcgtttcc caacatgaat ggtcagcgtg
     5041 ctttgcggcg gcagaacgta caattgcgcc agaccatctt ctccacgcgc ggcttctgcc
     5101 gatggaatgt tgataatctt gttgcctttg cccttcgaca gctgcggcag atcgcttacc
     5161 gggaacatca acatacggcc tgcctgagtg attgccagca gcatatcgga agcatcttca
     5221 atcaccaccg gcggcataac atgggcattt tccggtaagg tgatcaaagc cttacctgca
     5281 cggttacgcg ccaccagatc gttaaaggtg cagacgaaac cgtaacccgc atcggaagcc
     5341 atcagcagtt tctgatcgtc gctttccatc agcatatggt caacggtcgc cccaggcggc
     5401 aacgttaatt tgccggtgag tggttcgccc tgaccacgcg ccgacggcag cgtaatcggg
     5461 tcgatggcat agctacgacc ggtggaatca acaaacacta ccggttggtt gctcttacct
     5521 ttcaccgccg ctttgaagct atcacccgct ttataattca ggcccggcgc gtcgatatca
     5581 tggcctttag cgctgcgtac ccagcccatc agcgacagca caatggtgac aggttcagac
     5641 ggcagcatgt cgtgctcgct catcgctttc gcttcttcgc gttcctgcaa cggcgaacga
     5701 cgatcgtcac cgtaggcttg cgcgtctgcc tgcagttctt tcttcagcag gttattcatt
     5761 ttacgctcgg aaaccaaaat gccctgcaac tggtcgcgct ctttttccag ctcactctgc
     5821 tcaccgcgaa tcttcatctc ttccagtttg gcaagatgac gcagtttcag ttcgaggatc
     5881 gcttccgcct gggtttccgt aaggccaaac cgcgacatca gcgccggttt cggttcatct
     5941 tcgttacgaa tgatctcaat cacttcgtcg atgttgagaa acgccaccag caaaccttcc
     6001 aggatatgca ggcgcttgag gactttctcc agacgatagt tcagtcggcg gcgcacggta
     6061 tcgcgacgga acaccagcca ttcggagagg atttccagca ggtttttcac cgccggacga
     6121 ccatccagac cgatcatatt gaggttaatg cgatagctct tttccagatc ggtggtagcg
     6181 aagaggtggt tcatcacctg atccatatcc acgcggttgg aacgcggcac aatcaccaga
     6241 cgggtcgggt tctcgtggtc agattcatcg cgcagatcgt caaccatcgg cagctttttg
     6301 ttgcgcattt gcgcagcaat ttgctccagt acgcgcgcac ctgaaacctg atgcggcaat
     6361 gcgctgataa ccaccgcgcc atcttctttc ttccacaccg cgcgcatacg cactgaacca
     6421 cgtccgttct cgtagatttt acggatctcg gcgcgcgaag tgataatttc cgcttcagtc
     6481 ggataatccg gcccctgcac gatatccagc agctgatcga gcgtggtttt cggctggtcg
     6541 attaatgcga ttgccgcctg agctacttca cgcaggttat gcggtggaat atcggtcgcc
     6601 atgccgacgg caataccggt ggtgccgtta agcaaaatgt ttggcagacg ggcaggcagc
     6661 attttcggct cctgcaaagt gccgtcgaag tttggcaccc agtcagccgt cccctgcccc
     6721 agctcgctca atagcagttc ggaatatttc gacaaccggg attcggtgta acgcattgcc
     6781 gcgaacgatt tcggatcgtc cggcgcgccc cagttcccct gaccatcaac cagcggataa
     6841 cggtaagaga acggctgcgc catcaggacc atcgcttcat aacaggcact atcgccgtgc
     6901 ggatggtatt tacccagtac gtcaccgacg gtacgggccg attttttaaa tttggcgctg
     6961 gcattcaggc ccagttcaga catcgcatac acaatgcggc gctgaacagg tttaagacca
     7021 tcgccaataa acggcaacgc acggtccatg atcacgtaca tggagtagtt taagtaggcg
     7081 ttttccgtaa attcatgtag cgcaaggcgc tctgccatat cgctcattaa ttctgattcc
     7141 tcaacttatt cgccagcctt caaaggggca atattgccgg agatactaca ttatctgccg
     7201 cgtcgagtca caggagatca gcgtgaatag cgccgccgga cgtgctccga cggcgataaa
     7261 ggcatcaatg tgccttgata tacaactctt tgctgtagac ttgatcggca tagagggcag
     7321 cacaaggttg cgcccatgcc acaccactcg gcatgggggt ccgctccggg cgattcgaga
     7381 agttgaggtt atgcgagtcc agggaagcct aaagcatcaa agtagtcgac tgtaagtata
     7441 cggttcagcc cagtttagct gtgtctccac cagtgccccg cgcctcctgc cataagtgcg
     7501 gcttgctcat ggataccccc gagacggctt acggcgcacc ggataataaa tttcgcgtat
     7561 acatggtatt ccttaccgga gaaattataa gcccaacaac aatacgcctg tggcattgtt
     7621 cgctcaaact tatcgcgagt gatttggctc acactccggt aaagattccc catcacgcta
     7681 acggcacata aacatccgtt tccagtcgct gttctgtcat accttcggca agattggtgt
     7741 agtgaaacag aatcggtgct ttacgcattt tctcgccgct tgcaggcagc cagtggcgaa
     7801 tgatgcccca taccgtgtgt gaaatatcgt ccagctcgcc aacgtggcgg gccacggcat
     7861 aacgtccacc ggtaagttca ccattgctca caccataacg attatcggga atcggttcgc
     7921 taacgctgcc gcagatatca aagcgaaacg ctttcggtgc ggtggtggca gggtcgtccc
     7981 aggcgacgcc aaaagtctgg ctttggttaa caggagaaag ccccgtctct ttacgccaca
     8041 tgataaattt cgccacactg tagttgagca actcagggct acagcggtgc ggcaacatcg
     8101 ccacagggat tgatgggaaa tcgataatgt ttacatccag tgtcaggttt gtcattttgc
     8161 ctccctgcga aattattccc gggaacgcgt cccgggagcg gtaacaatta cggatttact
     8221 ttgcggatct gtttgacgtc aatttcaaca gaattccagt ctttatcgac ttccccctga
     8281 atctcaaccg tatctttcgg cgtcaccgtc acaccgttcc agcgtttgtg gtcgatatca
     8341 acattgatag taccgctggc atctttaaac acgtagagat cgtcagagat gcgttcaacg
     8401 atattgccgc gcagggttac ccaggtgtcg tcacgcaggg attttgcgct ttctacagtc
     8461 gttacgctgc cgttcggccc ctggaatcct ccggcctgac tttgcgttgc cgatgggcca
     8521 gaaaaaccgc cctgctctgc tgccatcacc ggtgcactgc acagggccat tactgcgatt
     8581 actgctgcga attttttcat gtttattact ccctttaatg tctgtttccg agcatttaac
     8641 aagatagtcc ttaacaactt cttaagggaa aaaaataaaa tttaatgctg tacagagcgc
     8701 gttacaacac ggtttactgg cagcaaatac ggttatcgca gggatgaaaa aatgcgaatt
     8761 ttactgatag aagatgacat gctgattggc gacggcatca aaactggcct tagtaaaatg
     8821 ggttttagtg tcgactggtt tacacaaggt cgtcagggaa aagaggcgct ttatagcgca
     8881 ccttatgatg cggtgatcct ggatttaacc ttaccaggca tggatggtcg cgatattttg
     8941 cgcgaatggc gagagaaagg tcagcgtgag ccggtactga tcctgaccgc gcgtgatgcg
     9001 ttagcggaac gtgtagaagg gctgcgtctg ggagctgacg attatctgtg taaacctttt
     9061 gcgttgatag aagtcgccgc caggctggag gctctgatgc gccgaaccaa cggccaggcc
     9121 agcaacgagc tgcgccacgg taacgtcatg ctcgaccccg gcaaacgtat cgccacgctg
     9181 gctagcgaac ccttaacact gaaaccaaaa gaatttgccc tgctggaatt actgatgcgt
     9241 aacgctggtc gggtactgcc gcgcaaactg attgaagaga aactgtatac ctgggacgaa
     9301 gaggtcacca gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa actcggtagt
     9361 gattttattc gtaccgtgca tggtattggc tacacattag gtgagaaatg aaatttaccc
     9421 aacgtcttag tctgcgcgtc aggctgacgc taatcttttt aattctggcc tcggtgacct
     9481 ggctgctttc cagctttgtc gcctggaaac aaacaacgga taacgtcgat gaattgttcg
     9541 acacccaact gatgctgttt gccaagcggt taagtacgct cgatctcaac gaaatcaacg
     9601 cggcggatcg catggcacag acgccaaata aattaaaaca cggtcatgtt gatgacgatg
     9661 cgctgacctt tgccatcttt acccacgacg gcagaatggt ccttaatgat ggcgataacg
     9721 gagaagatat tccctatagc tatcaacggg aaggttttgc tgacgggcaa ctggtcggtg
     9781 aagacgatcc ttggcgtttt gtctggatga cctcacctga tggcaaatat cgcatcgttg
     9841 ttggccagga atgggaatac cgtgaagaca tggcgctggc gattgttgcc gggcaattga
     9901 tcccgtggct ggtcgcactg ccgattatgt taatcatcat gatggtacta ctgggtcgtg
     9961 aactcgcgcc gctgaacaaa ctggcgctgg cactacgtat gcgtgaccct gactcggaaa
    10021 aaccactaaa cgcgactggc gtacccagcg aagtgcgacc actggttgag tcgctaaatc
    10081 aactgttcgc ccgcacacat gcgatgatgg ttcgagaacg acgctttacc tccgatgcag
    10141 ctcacgaact acgtagcccg ttaacagcgc tgaaagtgca aaccgaagtt gcgcagctct
    10201 ctgacgatga tccgcaggca cggaaaaaag cactgctcca attacattcc gggatcgatc
    10261 gcgctactcg tctggttgat caactgctca cgctatcgcg gctggactca ctggataacc
    10321 ttcaggacgt cgcggagatc ccgcttgaag atctcctgca atcgtcggtg atggatattt
    10381 accacacggc gcagcaggcg aaaattgacg tgcgactgac actcaatgtc cagggcatca
    10441 aacgcaccgg acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg gataacgccg
    10501 tgcgttacag tccacagggc agcgtggtgg acgtcacgct gaatgccgat aatttcatcg
    10561 tgagggataa cggccccggt gtgacaccag aggcactggc gcgaattggc gaacgcttct
    10621 atcgcccctc cggacaaacc gctaccggca gtggacttgg gctatcgatt gtccagcgaa
    10681 tcgccaagct gcatggcatg aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg
    10741 cgaaagtaag ctggtaaggg taagactttt gctaaattcg caaaagactt tgcacatttt
    10801 gctaatttca ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt
    10861 taaaaaatga gcaacattct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt
    10921 caactgaatg acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat
    10981 gttcgcatcg ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc
    11041 tgggctgatg tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg
    11101 aaaaaataca ttgatgatgt attcaccgaa ggtcacggga cgctgtatgc cagcgatggc
    11161 cgtacccgca aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa
    11221 tatatgcttt ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc
    11281 ttccacggcg ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt
    11341 ggcatggaac cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc
    11401 cgctatactg aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg
    11461 ctttagaagg agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa
    11521 catcaccgtc aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa
    11581 gaaggttgcc acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct
    11641 atggcaccgg attctatcgt gatgattgag cagtgggaaa gcatcgcgca ccttgaagcg
    11701 catctgcaaa ccccgcacat gaaggcgtac agcgaagccg taaaaggtga cgtgctggag
    11761 atgaatattc gtattctgca gccagggatt taatcctgcc ttgtttgccc ggccatcctg
    11821 accgggcaat gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac
    11881 cagttgcggc gatcttccga gcgtttcttc gccagtagca tgtccatcat cgcgtcagta
    11941 cgctgatcgt cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc
    12001 gtggtttcgc gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc
    12061 ggcttgcctt tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc
    12121 gcgtaataaa cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg
    12181 tgaccgtgtt tcaccaacgc gcggaagtgt tttacaaaca aagcgcagag cagcgtggca
    12241 atgtgcagac catcggagtc agcatccgcc aggatacaga tcttgccgta acgaagctgg
    12301 ctcaaatcgt cgctgtcagg atcgataccg atcgctaccg aaatatcgtg cacttcctgc
    12361 gaagccagca cttcgtcgga agagacttcc caggtgttaa ggatcttacc tttcagtggc
    12421 atgatcgcct gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct
    12481 tccacaagaa acagttcggt gcggttaagg tcctgcgcgg tacagtcagc cagtttgcca
    12541 ggcagcgccg gaccgctggt cagcttttta cgcaccactt ttttagccgc acgcatacga
    12601 cgctgggcgc tggaaatcgc catctctgcc agcaactccg ccgcctgaac gttctggttc
    12661 agccacagga taaaggcatc tttcaccacg ccagaaacga atgccgcgca ctgacgcgaa
    12721 gagagacgct ctttggtctg cccggcaaac tgcggatcct gcatttttac tgacagcaca
    12781 taggcgcagc gatcccagat atcttccgcc gacagcttta caccgcgcgg cagaatgttg
    12841 cggtattcac agaactcacg catcgcgtcc agcaggccct gacgcagacc attaacatgg
    12901 gtaccgccct gcatcgttgg gatcaggttg acgtagcttt cggtcagcag ttcaccgcct
    12961 tccggcagcc acagtagcgc ccagtccacc gcttcagtat cgccagcgaa attaccgata
    13021 aacggttttt ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga
    13081 ccgtcctgat agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca
    13141 acgccggggc acagtactgc tttggctttc agcacatgcg tcaggcgtga aacagaaaaa
    13201 cgcgggctat cgaagaaggt ttcatccggc cagaaatgga cgctggttcc ggtattgcgt
    13261 ttaccgcaag tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg
    13321 ttataaatct gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc
    13381 accgaaatcc ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta
    13441 ccgcctgcat gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga
    13501 atatccaccg gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaaa
    13561 ataacgtcca cgcgttttgc gtgacccgcc agtgcttcat ccacactgtt atcaatgact
    13621 tcttgcccca aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc
    13681 ggctcaagcc cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa
    13741 gttagtaatt cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt
    13801 gaaataatct tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca
    13861 ggaagcgtag tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag
    13921 cagccagatc aaatccggcg cttccagcgg gtcaatctgc atgactttaa gatcgtaaat
    13981 atggcgtgac tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata
    14041 gtccgtcagc agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa
    14101 acattgtgac aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga
    14161 atcgccgcca tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg
    14221 cggcaactgc ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa
    14281 caagctcgct tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga
    14341 catcagtagc cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc
    14401 tcggtggtca gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatggta
    14461 tccagcgtaa agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc
    14521 aggcggcgac cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat
    14581 ttgacgtgcg gaaacttcgc cagcacggta tccagttcgc ccgcgttacg cagactgtgt
    14641 tgatcgagcc aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg
    14701 cgttctggcg catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca
    14761 ccgtgcggca cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata
    14821 aacacgcgct tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg
    14881 aaatcgtggt tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg
    14941 aaatgctgat aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg
    15001 tcgaattcgt gctgatgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggtg
    15061 tttaccccta acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa
    15121 atcctgactc tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct
    15181 taggtttcac gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc
    15241 aaccagtccg ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta
    15301 ttaggataat cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc
    15361 accatcgcgt catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg
    15421 ggcgcagtct gttctattgt caccagggta gtgtatcggg tcgattccac aatcgtcagc
    15481 cgatattgtg cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt
    15541 aacaaacggc gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca
    15601 ggtgtgtaac gcttcattta tgcccactca ttttttaacg cttggtggtg cagctgcagc
    15661 cattgcaaag cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat
    15721 gcctgttccc ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga
    15781 ataccgcttg cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg
    15841 ccccccgggc ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagccccgcc
    15901 tcttcaatcg cttcgcgacg ggcaacatct tccacacttt caccctcttc aatcatcccg
    15961 gcaaccatct ccagtagcca gggggtttcg ctagtgtcgt acgcggcaat tcgaatctgc
    16021 tcaatcagca caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg
    16081 cgctcaaaaa tttcccgccg cacctcatga ctcatttgcc cgttgaatag actatgacga
    16141 aatctataaa gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct
    16201 acatcgtttt tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg
    16261 gcactaatag tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat
    16321 gtgttaaatt gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg
    16381 gcttctgcta gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac
    16441 aaggaatgca aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tctgggttca
    16501 gttcgttgag ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc
    16561 cggaattgcg taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc
    16621 gcagtccatt actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc
    16681 gcgacgcgaa cggcatcaac tcgaacgcga ccagtgcgtc cctgcagtta actcaatcca
    16741 tttttgatat gtcgaaatgg cgtgcgttaa cgctgcagga aaaagcagca gggattcagg
    16801 acgtcacata tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca
    16861 acgtgttgaa tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc
    16921 gtcaattaga tcaaaccacc caacgtttta acgtgggcct ggtagcgatc accgacgtgc
    16981 agaacgcccg cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgtaataacc
    17041 ttgataacgc ggtagagcag ctgcgccaga tcaccggtaa ctactatccg gaactggcgg
    17101 cgctgaatgt cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag
    17161 aagccgaaaa acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg
    17221 agcaaattcg ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg
    17281 ggatttctga cacctcttat agcggttcga aaactcgtgg tgccgctggt acccagtatg
    17341 acgacagcaa tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg
    17401 gcggaatggt taactcgcag gtgaaacagg cccagtacaa ctttgttggt gccagcgagc
    17461 aactggaaag cgcgcatcgt agcatcgtgc aaaccgtacg ttcctccttc aacaacatta
    17521 atgcatctat cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat
    17581 tagacgcgat ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg
    17641 caaccaccac gctgtacaac gctaagcaag agctggcaaa tgcgcgttat aactacctga
    17701 ttaatcagct gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac
    17761 tgaacaatgc gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc
    17821 cggaacagaa tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc
    17881 aaacatccgc acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac
    17941 gacggggctt cggccccgtc tgaacgtaag acaacgtaaa gatacgggtt atctgccgca
    18001 ttcttccccc ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc
    18061 actggtcccg gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca
    18121 aaaactggag cgcacgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc
    18181 tggctagctg tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt
    18241 cagctgcaaa cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag
    18301 aagccgaacg tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg
    18361 aaggtcagtg ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg
    18421 cccagcaatc cagcgggagt ttctggatgc cgctgatggc cggttacatg atggggcgtc
    18481 tgatgggcgg cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca
    18541 gcccggctta cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag
    18601 gccgcaccat gaccgtaccg aagacagcca tggcaccaaa accggcgacc actactaccg
    18661 ttacccgtgg cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa
    18721 ccggcacctc ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga
    18781 gcgcccggac tggcgtgaaa aagcccacga atacggtttc aattttcaca ccatgtacgg
    18841 cgagccgtac tggtgtgaag atgcttacta caagttgccc ctcgcccagg ttgaaaagct
    18901 ggaagaagtc accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc
    18961 cagcgatgag ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgtca
    19021 gtcatggctg actcaccagc catcgcttta ttcgcgtctt gatctggcat gggatggcac
    19081 tggcgaacct aaactgctgg aaaataacgc cgatacacca acgtcactat acgaggcggc
    19141 gttctttcag tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag
    19201 cgaccagttt aacagtctgc aagaaaagct aatcgatcgc tttgtcgagt tgcgtgaaca
    19261 gtatggcttc cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac
    19321 cattcagtat ttgcaggact gcgcgacgga agctgaaatc gctaccgagt tcctctacat
    19381 tgacgatatc gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc
    19441 caacctgttc aagctgtatc catgggaatt tatgttgcgt gagatgttct ccaccaagct
    19501 ggaggatgca ggcgtacgct ggctggaacc ggcgtggaag agcattatct ccaacaaagc
    19561 gcttctaccg ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt
    19621 tgcggaagat gatcatccgc aaatggaaaa atatgtggtt aaaccgatct tctcccgtga
    19681 aggcgcaaac gtgtcgatca ttgagaacgg caaaaccatt gaagcagcgg aaggtccgta
    19741 tggcgaagaa gggatgattg ttcagcaatt ccatccgtta ccgaaattcg gcgacagcta
    19801 tatgctgatt ggtagctggc tggtgaacga tcaacccgcc ggaattggca ttcgtgaaga
    19861 ccgtgcattg atcacccagg atatgtctcg gttttatcca catatttttg ttgagtaatc
    19921 aatgatgccg gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata
    19981 ctcaggctgc ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac
    20041 gcacctaaga catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca
    20101 ccttcatggt cgaggtaatt caccagagga tgctgctcta ccgccccttg ccacgtcaga
    20161 ttcgctttca catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc
    20221 cacttcactg tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca
    20281 tctcgcagcg ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg
    20341 tcgatactca actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc
    20401 caggaaccgt ggtcaaagcc cctggcttct ttatccagcg tcaccgggac cggcgctaac
    20461 agctcaacca gatgctgtgc cagcacaggc gaacccggag caggataatg cgtatcgtac
    20521 agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact
    20581 cctgttccac gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat
    20641 gtcatcccca acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga
    20701 ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact
    20761 aagggtgtca ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa
    20821 caatgatgat gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag
    20881 ttgtcgatgt cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc
    20941 gcgtttctcg gcgttctcgg acaaaaaccc tcgaaccgct tactggcgtt ttcgctaggt
    21001 tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca
    21061 gctgaaggaa tatcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctac
    21121 tttggcctgg accgcatgtt gccgcatgcg catccgcagg atttaatgca aaaatcggtg
    21181 cagccgttgc caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg
    21241 cataacttcc cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga
    21301 tttggcatcg cactggccgt cgcgttgcac aatatccctg aaggtctggc agtggcaggc
    21361 ccggtttatg cggcaacggg gtctaaacgc accgcgattc tgtgggcggg gatttctgga
    21421 ctagcagaaa ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg
    21481 gtggtcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga attaatgccg
    21541 ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg tggaatgtca
    21601 gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa acaacatcag
    21661 aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt ctaccttttc
    21721 ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca gtgcgtctct
    21781 gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca ccacaaccag
    21841 agcaatcaat acaatccagt accaggcgat aaacatcctt tacccttaat atttaacaat
    21901 actaaacatt tagcgcataa atttcacata tcctttttcg gatatattca tatggtcgca
    21961 taataacgga acaattatat gattttgatc ataatgaaaa agaatgcctg atattaatga
    22021 aattttctca tagaaagaga agcggaatta acatcttgca tagcagggca aaataaaaac
    22081 cgaagccagc tacaacttcg gtttcttgat taaggcagta attaagcagc ggttttcagc
    22141 tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca
    22201 tattgtgttt attggcaaac tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat
    22261 tagtcagctc acacagcaca ccagccggtt taaagcctgc cagcgtcatc aaatcaatag
    22321 tcgcttcagt atgaccgcca cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa
    22381 cgtggcccgg gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg
    22441 tcgtaatacg atcagcggca gaaacaccgg tagtcacacc ttcagccgct tcaatggtca
    22501 cggtaaaacc ggtgccatag gcgctggtgt tattttctac catcattggc agatcaagtt
    22561 gtttacggcg atcttcagtg atgcacaggc aaacaatacc gctaccgtgg cgaatggtca
    22621 gcgccatctg ctcaacggtc atggtttctg ccgggaagat catatcgcct tcgttttcac
    22681 ggtcttcatc atcaagcacc attacaccgc gtccttcacg cagcgcagcc agtgcatttt
    22741 caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaaa
    22801 ctcactaaaa ttatggttac cagaatcagg gcagtcttag gagtggcggc atatagccaa
    22861 aataacgtga gcgggtccat gcccgacaga atcgttactc tctcccatcc ggactctaac
    22921 cgtcggcccc ggaattacac cggatctgct gtcctttgag ttcgcaccca aagcgctcgc
    22981 gggctttcaa ctgagttgat ttaccgccgg tggggaattt cgccccgccc tgagaataag
    23041 cggattcact ataacgctaa tgattagcgg cagcaacgca tagcttcaca taattctggt
    23101 ttatgactta cccttatcgc actacaatgg tactcaacac ctatcagtac agggaaacta
    23161 caatgattga cccgaaaaaa attgagcaaa tcgctcgcca agttcacgaa tcaatgccta
    23221 aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc
    23281 agctgacgcg cctcgacctt gtaagccgtg aagagttcga cgtccaaacg caagtcctgt
    23341 tacggactcg tgagaaactg gcgctgcttg aacaaagaat gagcgaactg gaaaatcgca
    23401 gtactgagat taaaaagcag ccagatccag agactctccc cccaacgctg taaacatttg
    23461 tctttaatgc actgtaaatt atgaattaat cttctaattt acagtgcttt ctattacctt
    23521 atcgcttaat tccattcaga atatcaccac aaaaatgaat gagtgcaatt gaaaatataa
    23581 ttgttatgta tttttttaag ttttacttaa tccatattta gataaactaa tttcataaaa
    23641 cccgaaaagt aaaatataat atttgaatga ttagcaatcc ttaaataaca gttactaatc
    23701 attcattttc tacttatatt tatataaagg aatattatgt ctgcttttaa aaagtcactt
    23761 cttgttgctg gcgtagcaat gattctttcc aataacgtat ttgctgacga aggtcacggc
    23821 attgttaaat ttaaaggtga agttatctct gctccatgtt caattaaacc aggtgacgaa
    23881 gacttaactg taaatctggg tgaagtagca gataccgttc tgaaaagcga ccagaaatct
    23941 ttggctgagc ctttcactat tcacctgcaa gactgcatgt tgagccaggg tggaaccact
    24001 tattcaaaag cgaaggttac tttcaccaca gcgaatacta tgacgggtca gactgacctg
    24061 ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag gcgttggcgt acgtattctg
    24121 aatagccaga gcggcgaagt gactttaggt actccggtag taattacatt caacaatact
    24181 aacagctatc aggaactgaa cttcaaagtt cgtatggaat ccccgggcaa agatgctacc
    24241 ccgggcaacg tttacgctca ggctgactat aagattgctt acgagtaatc acattaggcc
    24301 gggttcgccc ggccacatca ttaacggatt aatgataagt ggaacagatg tataaaaaat
    24361 taaaattaac cacaataagc gaattgatta aaaatattta ttgttcatta tccgttctca
    24421 tcattggttg tgcgtcagct tatgccgttg aattcaacaa agatttaatc gaagccgaag
    24481 atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg ccaattaccc gtaggtaaat
    24541 actcattaaa cgctattatt aataataaaa ggacgccaat ccatctggac ctgcaatggg
    24601 tattaattga taaccaaact gcagtttgtc tgactccaga gcaattaaca ttattaggct
    24661 ttactgatga gattattgaa gaggctcagc aaaacctgat cgatggttgt taccctatcg
    24721 aaaaagaaaa acaaattaca acttatctcg ataaagggaa aatgcaatta tccatatctg
    24781 cacctcaggc atggttaaaa tacaaagatg caaactggac gcctcctgaa ctttgggatc
    24841 atggtattgc tggggcattt cttgactaca atttatatgc ctctcattat gcaccacatc
    24901 agggcgataa ttcacaaaat ataagttcct atgggcaggc tggggttaat cttggggcct
    24961 ggcgcctgcg tactgattac cagtacgatc agtcatttaa caatggcaaa agccaggcga
    25021 acaacctgga ttttccgcgt atttatttgt ttcgcccaat cccagcaatt aatgcaaaac
    25081 taactatagg tcaatacgat actgaatcct ctattttcga ctctttccat ttttctggcg
    25141 tttcgttgaa aagcgatgag aatatgttgc cgcctgatct acgtggttac gcaccgcaaa
    25201 tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc acagaacaac cgtattattt
    25261 atcaagaaaa tgttcctcct ggcccatttg ctattactaa tttattcaat acattacagg
    25321 ggcaactgga cgtcaaggtt gaagaagagg acgggcaagt cacgcaatgg caagttgcat
    25381 ctaatagtat tccttatctg acgcgtaaag ggcagattcg ctacaccact gctatgggta
    25441 aaccgaccag cgttggtggt gattccttac aacaaccctt cttctggact ggtgaattct
    25501 catggggttg gctgaacaat gtatccctgc atggtggttc agttttaaca aaccgtgatt
    25561 atcaatctct ggctgccggc gttggcttta accttaactc attgggttca ttatcttttg
    25621 atgtcacacg atctgatact cagttgcata atcaggataa agaaacgggt tatagctacc
    25681 gcgctaacta ttcaaaacgt tttgaatcta ccggtagcca gctcactttc gctggttacc
    25741 gtttctctga taaaaacttt gtgacaatga atgaatatat caatgacact aaccattaca
    25801 cgaatt
//
LOCUS       DAJTOR010000042        25215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTOR010000042 DAJTOR010000000
VERSION     DAJTOR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.042"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..403)
                     /locus_tag="OO249_001552"
     CDS             complement(<1..403)
                     /locus_tag="OO249_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5635897.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGTIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLP"
     gene            782..1093
                     /gene="rpsJ"
                     /locus_tag="OO249_001553"
     CDS             782..1093
                     /gene="rpsJ"
                     /locus_tag="OO249_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5635898.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1126..1755
                     /gene="rplC"
                     /locus_tag="OO249_001554"
     CDS             1126..1755
                     /gene="rplC"
                     /locus_tag="OO249_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5635899.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1766..2371
                     /gene="rplD"
                     /locus_tag="OO249_001555"
     CDS             1766..2371
                     /gene="rplD"
                     /locus_tag="OO249_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5635900.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2368..2670
                     /gene="rplW"
                     /locus_tag="OO249_001556"
     CDS             2368..2670
                     /gene="rplW"
                     /locus_tag="OO249_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5635901.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2688..3509
                     /gene="rplB"
                     /locus_tag="OO249_001557"
     CDS             2688..3509
                     /gene="rplB"
                     /locus_tag="OO249_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5635902.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3526..3804
                     /gene="rpsS"
                     /locus_tag="OO249_001558"
     CDS             3526..3804
                     /gene="rpsS"
                     /locus_tag="OO249_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5635903.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3819..4151
                     /gene="rplV"
                     /locus_tag="OO249_001559"
     CDS             3819..4151
                     /gene="rplV"
                     /locus_tag="OO249_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5635904.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4169..4870
                     /gene="rpsC"
                     /locus_tag="OO249_001560"
     CDS             4169..4870
                     /gene="rpsC"
                     /locus_tag="OO249_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5635905.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4883..5293
                     /gene="rplP"
                     /locus_tag="OO249_001561"
     CDS             4883..5293
                     /gene="rplP"
                     /locus_tag="OO249_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5635906.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5293..5484
                     /gene="rpmC"
                     /locus_tag="OO249_001562"
     CDS             5293..5484
                     /gene="rpmC"
                     /locus_tag="OO249_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5635907.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5484..5738
                     /gene="rpsQ"
                     /locus_tag="OO249_001563"
     CDS             5484..5738
                     /gene="rpsQ"
                     /locus_tag="OO249_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5635908.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5903..6274
                     /gene="rplN"
                     /locus_tag="OO249_001564"
     CDS             5903..6274
                     /gene="rplN"
                     /locus_tag="OO249_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5635909.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6285..6599
                     /gene="rplX"
                     /locus_tag="OO249_001565"
     CDS             6285..6599
                     /gene="rplX"
                     /locus_tag="OO249_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5635910.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6614..7153
                     /gene="rplE"
                     /locus_tag="OO249_001566"
     CDS             6614..7153
                     /gene="rplE"
                     /locus_tag="OO249_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5635911.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7168..7473
                     /gene="rpsN"
                     /locus_tag="OO249_001567"
     CDS             7168..7473
                     /gene="rpsN"
                     /locus_tag="OO249_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5635912.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7507..7899
                     /gene="rpsH"
                     /locus_tag="OO249_001568"
     CDS             7507..7899
                     /gene="rpsH"
                     /locus_tag="OO249_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5635913.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7912..8445
                     /gene="rplF"
                     /locus_tag="OO249_001569"
     CDS             7912..8445
                     /gene="rplF"
                     /locus_tag="OO249_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5635914.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRIKEAKKK"
     gene            8455..8808
                     /gene="rplR"
                     /locus_tag="OO249_001570"
     CDS             8455..8808
                     /gene="rplR"
                     /locus_tag="OO249_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5635915.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8823..9326
                     /gene="rpsE"
                     /locus_tag="OO249_001571"
     CDS             8823..9326
                     /gene="rpsE"
                     /locus_tag="OO249_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5635916.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9330..9509
                     /gene="rpmD"
                     /locus_tag="OO249_001572"
     CDS             9330..9509
                     /gene="rpmD"
                     /locus_tag="OO249_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5635917.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9513..9947
                     /gene="rplO"
                     /locus_tag="OO249_001573"
     CDS             9513..9947
                     /gene="rplO"
                     /locus_tag="OO249_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5635918.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9955..11286
                     /gene="secY"
                     /locus_tag="OO249_001574"
     CDS             9955..11286
                     /gene="secY"
                     /locus_tag="OO249_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5635919.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11318..11434
                     /gene="rpmJ"
                     /locus_tag="OO249_001575"
     CDS             11318..11434
                     /gene="rpmJ"
                     /locus_tag="OO249_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5635920.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11581..11937
                     /gene="rpsM"
                     /locus_tag="OO249_001576"
     CDS             11581..11937
                     /gene="rpsM"
                     /locus_tag="OO249_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5635921.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11954..12343
                     /gene="rpsK"
                     /locus_tag="OO249_001577"
     CDS             11954..12343
                     /gene="rpsK"
                     /locus_tag="OO249_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5635922.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12377..12997
                     /gene="rpsD"
                     /locus_tag="OO249_001578"
     CDS             12377..12997
                     /gene="rpsD"
                     /locus_tag="OO249_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5635923.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13023..14012
                     /gene="rpoA"
                     /locus_tag="OO249_001579"
     CDS             13023..14012
                     /gene="rpoA"
                     /locus_tag="OO249_001579"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5635924.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14053..14436
                     /gene="rplQ"
                     /locus_tag="OO249_001580"
     CDS             14053..14436
                     /gene="rplQ"
                     /locus_tag="OO249_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5635925.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14543..14911
                     /locus_tag="OO249_001581"
     CDS             14543..14911
                     /locus_tag="OO249_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR5635926.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGF
                     STDFLRGDYADKLLDKINDN"
     gene            14922..15347
                     /gene="zntR"
                     /locus_tag="OO249_001582"
     CDS             14922..15347
                     /gene="zntR"
                     /locus_tag="OO249_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR5635927.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15403..15621
                     /gene="arfA"
                     /locus_tag="OO249_001583"
     CDS             15403..15621
                     /gene="arfA"
                     /locus_tag="OO249_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR5635928.1"
                     /translation="MSRYQHTKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="OO249_001584"
     CDS             complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="OO249_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR5635929.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="OO249_001585"
     CDS             complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="OO249_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5635930.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="OO249_001586"
     CDS             complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="OO249_001586"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5635931.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRHH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="OO249_001587"
     CDS             complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="OO249_001587"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5635932.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERYIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19853..20362)
                     /gene="def"
                     /locus_tag="OO249_001588"
     CDS             complement(19853..20362)
                     /gene="def"
                     /locus_tag="OO249_001588"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5635933.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20492..21616
                     /gene="dprA"
                     /locus_tag="OO249_001589"
     CDS             20492..21616
                     /gene="dprA"
                     /locus_tag="OO249_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5635934.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     YALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVSPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21588..22061
                     /locus_tag="OO249_001590"
     CDS             21588..22061
                     /locus_tag="OO249_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5635935.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22090..22632
                     /locus_tag="OO249_001591"
     CDS             22090..22632
                     /locus_tag="OO249_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR5635936.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22637..23209
                     /gene="tsaC"
                     /locus_tag="OO249_001592"
     CDS             22637..23209
                     /gene="tsaC"
                     /locus_tag="OO249_001592"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5635937.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVLGETGGRLNPSEIRDALTGELFRQG"
     gene            23214..24032
                     /gene="aroE"
                     /locus_tag="OO249_001593"
     CDS             23214..24032
                     /gene="aroE"
                     /locus_tag="OO249_001593"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5635938.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24029..24286
                     /locus_tag="OO249_001594"
     CDS             24029..24286
                     /locus_tag="OO249_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5635939.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24262..24816)
                     /locus_tag="OO249_001595"
     CDS             complement(24262..24816)
                     /locus_tag="OO249_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5635940.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGGVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6444 a         5992 c         6564 g         6215 t
ORIGIN      
        1 aaggcagcgg gttttttaat gattcttttc ctctcatgag cagaccaata actacggctc
       61 cgcaagcgaa tgaggcagca aggaaaacca gtcgtggtaa gaaggcccat gaatgccagg
      121 cgccaagggc ggcgagaaac ttcacatcac catagcctaa tccttcttta tgtcgcagta
      181 tgcgataccc ccagtaaatg acagcgaatg tgccgtaacc aacaattgtc ccccataacg
      241 catcggctaa accatcagga tgacaaactt gatagaacaa aagacctgac cagagtaacg
      301 ggcacgtaaa tctgtcaggt aacaagccat gctttgcatc ccagaaaaaa agtagggctg
      361 ataaacaagc gtacaaaatc agaaagggaa gggtagtgtc cataaaacat cctctgtaat
      421 tggagtatca ccatcgaata actccggatg ctcagcaaca gagagagcgc tgatttgtta
      481 acctgattcc ggtgaaaaac gctatcttca caaccttaca aatttcgtgg aatgcagctc
      541 gcaatctgcg cgttgcgatt aatttctacc cgatgcttgc gtcaggctca gatttgcgta
      601 taatgcgcgg gcttgtcgta gttgacagca ggttcaatct gaacctcagt tagccgaatt
      661 tggctaccta acaatgctcc caatcgggga gctacgtaag aacggttaca ctctcccatc
      721 aatcgtaatg ggtctgagga gtaatcattt tcgtttataa aataattgga gctctggtct
      781 catgcagaac caaagaatcc gtatccgcct gaaagcgttt gatcatcgtc tgatcgatca
      841 agcaaccgcg gaaatcgtcg agactgccaa gcgcactggt gcgcaggtcc gtggtccgat
      901 cccgctgccg acacgcaaag agcgcttcac tgttctgatc tccccgcacg tcaacaaaga
      961 cgcgcgcgat cagtacgaaa tccgtactca cttgcgtctg gttgacatcg ttgagccaac
     1021 cgagaaaacc gttgatgctc tgatgcgtct ggatctggct gccggtgtag acgtgcagat
     1081 cagcctgggt taatcaggtc attgagcgat tgagaggttg aaacaatgat tggtttagtc
     1141 ggtaaaaaag tgggtatgac ccgtatcttc acagaagacg gcgtttctat cccagtaacc
     1201 gtaatcgaag ttgaagcaaa ccgcgttact caggttaaag acctggctaa cgatggctac
     1261 cgtgctattc aggtgaccac cggtgctaaa aaagctaacc gtgtgaccaa gcctgaagct
     1321 ggccacttcg ctaaagctgg cgtagaagct ggccgtggtc tgtgggaatt ccgcctggct
     1381 gaaggcgaag agttcactgt aggtcagagc attagcgttg aactgtttgc tgacgttaaa
     1441 aaagttgacg taactggcac ctctaaaggt aaaggtttcg caggtaccgt taagcgctgg
     1501 aacttccgta cccaggacgc tactcacggt aactccttgt ctcaccgcgt tccgggttct
     1561 atcggtcaga accagactcc gggcaaagtg ttcaaaggca agaaaatggc aggtcagatg
     1621 ggtaacgaac gtgtaaccgt tcagagcctt gacgtagtac gcgttgacgc tgagcgcaac
     1681 ctgctgctgg ttaaaggtgc tgtcccgggt gcaaccggta gcgacctgat cgttaaacca
     1741 gctgtgaagg cgtaaggaga tagcaatgga attagtattg aaagacgcgc agagcgcgct
     1801 gactgtttcc gaaactacct tcggtcgtga tttcaacgaa gcgctggttc accaggttgt
     1861 tgttgcttat gcagctggtg ctcgtcaggg tactcgtgct cagaagactc gtgctgaagt
     1921 aactggttcc ggtaaaaaac cgtggcgcca gaaaggcacc ggccgtgcgc gttctggttc
     1981 tatcaagagc ccgatctggc gttctggtgg cgtgaccttt gctgctcgtc cgcaggacca
     2041 cagtcaaaaa gttaacaaga agatgtaccg cggcgcgctg aaaagcatcc tgtccgaact
     2101 ggtacgtcag gatcgtctga tcgttgtcga gaagttctct gtagaagcgc cgaaaactaa
     2161 gctgctggca cagaaactga aagacatggc tctggaagat gtgctgatca tcaccggtga
     2221 gctggacgaa aacctgttcc tggctgcgcg caacctgcac aaggttgacg tacgcgatgc
     2281 aactggtatc gacccggtta gcctgatcgc cttcgacaaa gtcgtaatga ctgctgatgc
     2341 tgttaagcaa gttgaggaga tgctggcatg attcgtgaag aacgtctgct gaaggtgctg
     2401 cgtgcaccgc acgtttctga aaaagcgtct actgcgatgg aaaaatccaa caccatcgta
     2461 ctcaaagttg ctaaagacgc gaccaaagca gaaatcaaag ctgctgtgca gaaactgttt
     2521 gaagtcgaag tcgaagtcgt taacaccctg gtagttaaag ggaaagttaa acgtcacgga
     2581 cagcgtatcg gtcgtcgtag cgactggaaa aaagcttacg tcaccctgaa agaaggccag
     2641 aatctggact tcgttggcgg cgctgagtaa gtcggaggag taatacaatg gcagttgtta
     2701 aatgtaaacc gacatctccg ggtcgtcgcc acgtagttaa agtggttaac cctgagctgc
     2761 acaagggcaa accttttgct ccgttgctgg aaaaaaacag caaatccggt ggtcgtaaca
     2821 acaatggccg tatcaccact cgtcatatcg gtggtggcca caagcaggct taccgtattg
     2881 ttgacttcaa acgcaacaaa gacggtatcc cggcagttgt tgaacgtctt gagtacgatc
     2941 cgaaccgttc cgcgaacatc gcgctggttc tgtacaaaga cggtgaacgc cgttacatcc
     3001 tggcccctaa aggcctgaaa gctggcgacc agattcagtc tggcgttgat gctgcaatca
     3061 aaccaggtaa caccctgccg atgcgcaaca tcccggttgg ttctactgtt cataacgtag
     3121 aaatgaaacc aggtaaaggc ggtcagctgg cacgttccgc tggtacttac gttcagatcg
     3181 ttgctcgtga tggtgcttat gtcaccctgc gtctgcgttc tggtgaaatg cgtaaagtag
     3241 aagcagactg ccgtgcaact ctgggcgaag ttggcaatgc tgagcatatg ctgcgcgttc
     3301 tgggtaaagc aggtgctgca cgctggcgtg gtgttcgtcc gaccgttcgc ggtaccgcga
     3361 tgaacccggt agaccaccca catggtggtg gtgaaggtcg taactttggt aagcacccgg
     3421 taactccgtg gggcgttcag accaaaggta agaagacccg cagcaacaag cgtactgata
     3481 aattcatcgt acgtcgccgt agcaaataat tttagaggat aagccatgcc acgttctctc
     3541 aagaaaggtc cttttattga cctgcacttg ctgaagaagg tagagaaagc ggtggaaagc
     3601 ggagacaaga agcccctgcg cacttggtcc cgtcgttcaa cgatctttcc taacatgatc
     3661 ggtttgacca tcgctgtcca taatggtcgt cagcacgttc cggtatttgt aaccgacgaa
     3721 atggtcggtc acaaactggg tgaattcgca ccgactcgta cttatcgcgg ccacgctgct
     3781 gataaaaaag cgaagaagaa ataaggtagg aggaagagat ggaaactatc gctaaacatc
     3841 gccatgctcg ttcttctgct cagaaggttc gccttgttgc tgacctgatt cgcggtaaga
     3901 aagtgtcgca ggctctggat attttgacct acaccaacaa gaaagcggct gtactggtca
     3961 agaaagttct ggaatctgcc attgctaacg ctgaacacaa cgatggcgct gacattgacg
     4021 atctgaaagt tacgaaaatt ttcgtagacg aaggcccgag catgaagcgc attatgccgc
     4081 gtgcaaaagg tcgtgcagat cgcatcctga agcgcaccag ccacatcact gtggttgtgt
     4141 ccgatcgctg agactctgga gactagcaat gggtcagaaa gtacatccta atggtattcg
     4201 cctgggtatt gtaaaaccat ggaactctac ctggtttgcg aacaccaaag aattcgctga
     4261 caacctggac agcgatttta aagtacgtca gtacctgact aaggaactgg ctaaagcgtc
     4321 cgtatctcgt atcgttatcg agcgtccggc taagagcatc cgtgtaacca ttcacactgc
     4381 tcgcccgggt atcgttatcg gtaaaaaagg tgaagacgta gaaaaactgc gtaaggtcgt
     4441 agcggacatc gctggcgttc ctgcacagat caacatcgcc gaagttcgta agcctgaact
     4501 ggacgcaaaa ctggttgctg acagcatcac ttctcagctg gaacgtcgcg ttatgttccg
     4561 tcgtgctatg aagcgtgctg tacagaacgc aatgcgtctg ggcgctaaag gtattaaagt
     4621 tgaagttagc ggccgtctgg gcggcgcgga aatcgcacgt accgaatggt accgcgaagg
     4681 tcgcgtaccg ctgcacactc tgcgtgctga catcgactac aacacctctg aagcgcacac
     4741 cacttacggt gtaatcggcg ttaaagtgtg gatcttcaaa ggcgagatcc tgggtggtat
     4801 ggctgctgtt gaacaaccgg aaaaaccggc tgctcagcct aaaaagcagc agcgtaaagg
     4861 ccgtaaataa ggagcgtcgc tgatgttaca accaaagcgt acaaaattcc gtaaaatgca
     4921 caaaggccgt aaccgcggtc tggcgcaggg tacggatgtt agcttcggca gcttcggtct
     4981 gaaagctgtt ggccgtggtc gtctgactgc ccgtcagatc gaagcagcac gtcgtgctat
     5041 gacccgtgca gttaagcgtc aaggtaagat ctggatccgt gtgttcccgg acaaaccgat
     5101 cactgaaaag ccgctggcag tgcgtatggg taaaggtaaa ggtaacgtgg agtattgggt
     5161 tgccttgatt cagccgggta aagtcctgta tgaaatggac ggtgttccgg aagagctggc
     5221 ccgtgaagca ttcaagctgg cagcagcgaa actgccgatt aaaaccacct ttgtaactaa
     5281 gacggtgatg taatgaaagc aaaagagctg cgtgagaaga gcgttgaaga gctgaacacc
     5341 gagctgctga acctgctgcg tgagcagttc aacctgcgta tgcaggctgc aagtggccag
     5401 ctgcaacagt ctcacctgtt gaagcaagtg cgtcgcgatg tcgcacgcgt taagacttta
     5461 ctgaacgaga aggctggtgc gtaatgaccg ataaaatccg tactctgcaa ggtcgcgttg
     5521 ttagcgacaa aatggagaaa tccattgttg ttgctatcga acgttttgtg aaacacccga
     5581 tctacggtaa attcatcaag cgtacgacca aactgcacgt acatgacgag aacaacgaat
     5641 gcggtatcgg tgacgtggtt gaaatccgcg aatgccgtcc gctgtccaag actaaatcct
     5701 ggacgctggt tcgcgttgta gagaaagcgg ttctgtaata cagtacactc tctcgatacg
     5761 aataaacggc tcagaaatga gccgtttatt ttttctaccc atatccttga agcggtgtta
     5821 taatgccgcg ccctcgatat ggggattttt aacgacctga ttttcgggtc tcagtagtag
     5881 ttgacattag cggagcacta aaatgatcca agaacagact atgctgaacg tcgccgacaa
     5941 ctccggtgca cgtcgcgtaa tgtgtatcaa ggttctgggt ggctcgcacc gtcgctacgc
     6001 aggcgtaggc gacatcatca agatcaccat caaagaagca attccgcgtg gtaaggtcaa
     6061 aaaaggtgat gtgctgaagg cggtagtggt gcgcaccaag aagggtgttc gtcgcccgga
     6121 cggttctgtc attcgcttcg atggtaatgc ttgtgttctt ctgaacaaca acagcgagca
     6181 gcctatcggt acgcgtattt ttgggccggt aactcgtgag cttcgtagtg agaagttcat
     6241 gaaaattatc tctctggcac cagaagtact ctaaggagcg aatcatggca gcgaaaatcc
     6301 gtcgtgatga cgaagttatc gtgttaaccg gtaaagataa aggtaaacgc ggtaaagtta
     6361 agaatgtcct gtcttccggc aaggtcattg ttgaaggtat caacctggtt aagaaacatc
     6421 agaagccggt tccggccctg aaccaaccgg gtggcatcgt tgaaaaagaa gccgctattc
     6481 aggtttccaa cgtagcaatc ttcaatgcgg caaccggcaa ggctgaccgt gtaggcttta
     6541 gattcgaaga cggcaaaaaa gtccgtttct tcaagtctaa cagcgaaact atcaagtaat
     6601 ttggagtagt acgatggcga aactgcatga ttactacaaa gacgaagtag ttaaaaaact
     6661 catgactgag tttaactaca attctgtcat gcaagtccct cgggtcgaga agatcaccct
     6721 gaacatgggt gttggtgaag cgatcgctga caaaaaactg ctggataacg cagcagcaga
     6781 cctggcagca atctccggtc aaaaaccgct gatcaccaaa gcacgcaaat ctgttgcagg
     6841 cttcaaaatc cgtcagggct atccgatcgg ctgtaaagta actctgcgtg gcgaacgcat
     6901 gtgggagttc tttgagcgcc tgatcactat tgctgtacct cgtatccgtg acttccgtgg
     6961 cctgtccgct aagtctttcg acggtcgtgg taactacagc atgggtgtcc gtgagcagat
     7021 catcttccca gaaatcgact acgataaagt cgaccgcgtt cgtggtttgg atattaccat
     7081 taccactact gcgaaatctg acgaagaagg ccgcgctctg ctggctgcct ttgacttccc
     7141 gttccgcaag taaggtaggg ttactaaatg gctaagcaat caatgaaagc acgcgaagta
     7201 aaacgcgtag ctttagctga taaatacttc gcgaaacgcg ctgaactgaa agcgatcatc
     7261 tctgatgtga acgcttccga cgaagatcgt tggaacgctg ttctcaagct gcagactctg
     7321 ccgcgtgatt ccagcccgtc tcgtcagcgt aaccgctgcc gtcaaacagg tcgtccgcat
     7381 ggtttcctgc ggaagttcgg gttgagccgt attaaggtcc gtgaagccgc tatgcgcggt
     7441 gaaatcccgg gtctgaaaaa ggctagctgg taattgtcac caattgaatc acgggagtaa
     7501 agacagatga gcatgcaaga tccgatcgcg gatatgctga cccgtatccg taacggtcag
     7561 gccgcgaaca aagctgcggt caccatgcct tcctccaagc tgaaagtggc aatcgccaac
     7621 gtgctgaagg aagaaggttt tattgaagat tttaaagttg aaggcgacac caagcctgaa
     7681 ctggaactta ctctgaagta tttccagggc aaagctgttg tagaaagcat tcagcgtgtc
     7741 agccgcccag gtctgcgcat ctataaacgt aaagatgagc tgccgaaagt tatggcgggt
     7801 ctgggtatcg cagttgtttc tacctctaaa ggtgttatga ctgatcgtgc agcgcgccag
     7861 gctggtcttg gtggcgaaat tatctgctac gtagcctaat cggaggaaaa aatgtctcgt
     7921 gttgctaaag caccggtcgt tgttcctgcc ggcgttgacg taaaaatcaa cggtcaggtt
     7981 attacgatca aaggtaaaaa cggcgagctg actcgtactc tcaacgatgc tgttgaagtt
     8041 aaacatgcag ataataccct gaccttcggt ccgcgtgatg gttacgcaga cggttgggca
     8101 caggctggta ccgcgcgtgc cctgctgaac tcaatggtta tcggtgttac cgaaggcttc
     8161 actaagaagc tgcagctggt tggtgtaggt taccgtgcag cggttaaagg taatgtgatt
     8221 aacctgtctc tgggtttctc tcatcctgtt gaccatcagc tgcctgcggg tatcactgct
     8281 gaatgtccga ctcagactga aatcgtgctg aaaggcgctg ataagcaggt gatcggccag
     8341 gttgcagcgg atctgcgcgc ctaccgtcgt cctgagcctt ataaaggcaa gggtgttcgt
     8401 tacgccgacg aagtcgtgcg tatcaaagag gctaagaaga agtaaggtaa cactatggat
     8461 aagaaatctg ctcgtatccg tcgtgcgacc cgcgcacgcc gcaagctcca ggagctgggc
     8521 gcaactcgcc tggtggtaca tcgtaccccg cgtcacattt acgcacaggt aattgcaccg
     8581 aacggttctg aagttctggt agctgcttct actgtagaaa aagctatcgc tgaacaactg
     8641 aagtacaccg gtaacaaaga cgcggctgca gctgtgggta aagctgtcgc tgaacgcgct
     8701 ctggaaaaag gcatcaaaga tgtatccttt gaccgttccg ggttccaata tcatggtcgt
     8761 gtccaggcac tggcagatgc tgcccgtgaa gctggccttc agttctaagg tagaggtgta
     8821 agatggctca catcgaaaaa caagctggcg aactgcagga aaagctgatc gcggtaaacc
     8881 gcgtatctaa aaccgttaaa ggtggtcgta ttttctcctt cacagctctg actgtagttg
     8941 gcgatggtaa cggtcgcgtt ggttttggtt acggtaaagc gcgtgaagtt ccagcagcga
     9001 tccagaaagc gatggaaaaa gcccgtcgca atatgattaa cgtcgcgctg aataacggca
     9061 ctctgcaaca ccctgttaaa ggtgttcaca cgggttctcg cgtattcatg cagccggctt
     9121 ccgaaggtac cggtatcatc gccggtggtg caatgcgcgc cgttctggaa gtcgctgggg
     9181 ttcataacgt tctggctaaa gcatatggtt ccaccaaccc gatcaacgtg gttcgtgcaa
     9241 ctattgatgg cctggaaaat atgaactctc cagaaatggt cgctgccaag cgtggtaaat
     9301 ccgttgaaga aattctgggg aaataaacca tggcaaagac tattaaaatt actcaaaccc
     9361 gcagtgcaat cggtcgtctg ccgaaacaca aggcaacgct gcttggcctg ggtctgcgtc
     9421 gtattggtca caccgtagag cgcgaggata ctcctgctat tcgcggtatg atcaacgcgg
     9481 tttccttcat ggttaaagtt gaggagtaag agatgcgttt aaatactctg tctccggccg
     9541 aaggctccaa aaaggcgggt aaacgcttgg gtcgtggtat cggttctggc ctcggtaaaa
     9601 ccggtggtcg tggtcacaaa ggtcagaagt ctcgttctgg cggtggcgta cgtcgcggtt
     9661 tcgagggtgg tcagatgcct ctgtaccgtc gtctgccgaa attcggcttc acttctcgta
     9721 aagcagcgat tacagccgaa gttcgtctgt ctgacctggc taaagtagaa ggcggtgttg
     9781 tagacctgaa cacgctgaaa gcggctaaca ttatcggtat ccagatcgag ttcgcgaaag
     9841 tgatcctggc tggcgaagta acgactccgg taactgttcg tggcctgcgt gttactaaag
     9901 gcgctcgtgc tgctatcgaa gctgctggcg gtaaaatcga ggaataagta gcagatggct
     9961 aaacaaccgg gattagattt tcaaagtgcc aaaggtggct taggcgagct gaaacgcaga
    10021 ctgctgtttg ttatcggtgc gctgattgtg ttccgtattg gctcttttat tccgatccct
    10081 ggtattgatg ccgctgtact tgccaaactg cttgagcaac agcgaggcac catcattgag
    10141 atgtttaaca tgttctctgg tggtgctctc agccgtgctt ctatctttgc tctggggatc
    10201 atgccgtata tttcggcgtc gatcattatc cagctgctga cggtggttca cccaacgttg
    10261 gcggaaatta agaaagaagg ggagtctggt cgtcgtaaga tcagccagta cacccgctac
    10321 ggtactctgg tgctggcaat attccagtcg atcggtattg ctaccggtct gccgaatatg
    10381 cctggtatgc aaggcctggt gattaacccg ggctttgcat tctacttcac cgctgttgta
    10441 agtctggtca caggaacgat gttcctgatg tggttgggcg aacagatcac tgaacgaggt
    10501 atcggcaacg gtatttcaat cattatcttc gccggtattg tcgcgggact cccgccagcc
    10561 attgcccata ctatcgagca agcgcgtcaa ggcgacctgc acttcctcgt gttgctgttg
    10621 gttgcagtat tagtatttgc agtgacgttc tttgttgtat ttgttgagcg tggtcaacgc
    10681 cgcattgtgg taaactacgc gaaacgtcag caaggtcgtc gtgtctatgc tgcacagagc
    10741 acacatttac cgctgaaagt gaatatggcg ggggtaatcc cggcaatctt cgcttccagt
    10801 attattctgt tcccggcgac catcgcgtca tggttcgggg gcggtactgg ttggaactgg
    10861 ctgacaacaa tttcgctgta tttgcagcct gggcaaccgc tttatgtgtt actctatgcg
    10921 tctgcaatca tcttcttctg tttcttctac acggcgttgg ttttcaaccc gcgtgaaaca
    10981 gcagataacc tgaagaagtc cggtgcattt gtaccaggaa ttcgtccggg agagcaaacg
    11041 gcgaagtata tcgataaagt aatgacccgc ctgaccctgg ttggtgcgct gtatattacc
    11101 tttatctgcc tgatcccgga gttcatgcgt gatgcaatga aagtaccgtt ctacttcggt
    11161 gggacctcac tgcttatcgt tgttgtcgtg attatggact ttatggctca agtgcaaact
    11221 ctgatgatgt ccagtcagta tgagtctgca ttgaagaagg cgaacctgaa aggctacggc
    11281 cgataattgg tcgcccgaga agttacggag agtaaaaatg aaagttcgtg cttccgtcaa
    11341 gaaattatgc cgtaactgca aaatcgttaa gcgtgatggt gtcatccgtg tgatttgcag
    11401 tgccgagccg aagcataaac agcgccaagg ctgatttttt cgcatatttt tcttgcaaag
    11461 ttgggttgag ctggctagat tagccagcca atcttttgta tgtctgtgcg tttccatttg
    11521 agtatcctga aaacgggctt ttcagcatgg aacgtacata ttaaatagta ggagtgcata
    11581 gtggcccgta tagcaggcat taacattcct gatcataagc atgccgtaat cgcattaact
    11641 tcgatttatg gcgtcggcaa gacccgttct aaagccatcc tggctgcagc gggtatcgct
    11701 gaagatgtta agatcagtga gctgtctgaa ggacaaatcg acacgctgcg tgacgaagtt
    11761 gccaaatttg tcgttgaagg tgatctgcgc cgtgaaatca gcatgagcat caagcgcctg
    11821 atggatcttg gttgctatcg cggcttgcgt catcgtcgtg gtctcccggt tcgcggtcag
    11881 cgtaccaaga ccaacgcacg tacccgtaag ggtccgcgca aaccgatcaa gaaataatcg
    11941 gggtgattga ataatggcaa aggcaccaat tcgtgcacgt aaacgtgtaa gaaaacaagt
    12001 ctctgacggc gtggctcata tccatgcttc tttcaacaac accatcgtga ctatcactga
    12061 tcgtcagggt aacgcgttgg gttgggcaac agccggtggt tccggtttcc gtggttctcg
    12121 caaatccact ccgtttgcag ctcaggttgc agcagagcgt tgcgctgacg ccgtgaaaga
    12181 atacggcatc aagaatctgg aagttatggt taaaggtccg ggtccaggcc gcgaatctac
    12241 tattcgtgct ctgaacgccg caggtttccg catcactaac attactgatg tgactccgat
    12301 ccctcataac ggttgtcgtc cgccgaaaaa acgtcgcgta taacgcctcg ttttccaggt
    12361 ttgttggaga aagaaaatgg caagatattt gggtcctaag ctcaagctga gccgtcgtga
    12421 gggcaccgac ttattcctta agtctggcgt tcgcgcgatc gataccaagt gtaaaattga
    12481 acaagctcct ggccagcacg gtgcgcgtaa accgcgtctg tctgactatg gtgtgcagtt
    12541 gcgtgaaaag caaaaagttc gccgtatcta tggtgtgctg gagcgtcagt tccgtaacta
    12601 ctacaaagaa gcagcacgtc tgaaaggcaa caccggtgaa aacctgttgg ctctgctgga
    12661 aggtcgtctg gacaacgttg tataccgtat gggcttcggt gccactcgtg cagaagcacg
    12721 tcagctggtt agccataaag caattatggt aaacggtcgt gttgttaata tcgcttctta
    12781 tcaggttagt ccgaatgacg ttgtaagcat tcgtgagaaa gcgaagaagc agtctcgcgt
    12841 gaaagccgct ctggagctgg ctgagcagcg tgaaaagcca acttggctgg aagttgatgc
    12901 tggcaagatg gaaggtacgt ttaagcgtaa gccggagcgt tctgatctgt ctgcggacat
    12961 taacgaacac ctgatcgtcg agctttactc caagtaaagc ttagtaccaa agagaggaca
    13021 caatgcaggg ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc gagcaagtga
    13081 gttcgacgca cgccaaggtg acccttgagc ctttagagcg tggctttggc catactctgg
    13141 gtaacgcact gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg accgaggttg
    13201 agattgatgg tgtactacat gagtacagca ccaaagaagg cgttcaggaa gatatcctgg
    13261 aaatcctgct caacctgaaa gggctggcgg tgagagttca gggcaaagat gaagttattc
    13321 ttaccttgaa taaatctggc attggccctg tgactgcagc cgatatcacc cacgacggtg
    13381 atgtcgaaat cgtcaagccg cagcacgtga tctgccacct gaccgatgag aacgcgtcta
    13441 ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt gccggcttct acccgaattc
    13501 attcggaaga agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc tacagccctg
    13561 tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc gacctggaca
    13621 agctggtcat cgaaatggaa accaacggca caatcgatcc tgaagaggcg attcgtcgtg
    13681 cggcaaccat tctggctgaa caactggaag ctttcgttga cttacgtgat gtacgtcagc
    13741 ctgaagtgaa agaagagaaa ccagagttcg atccgatcct gctgcgccct gttgacgatc
    13801 tggaattgac tgtccgctct gctaactgcc ttaaagcaga agctatccac tatatcggtg
    13861 atctggtaca acgtaccgag gttgagctcc ttaaaacgcc taaccttggt aaaaaatctc
    13921 ttactgagat taaagacgtg ctggcttccc gtggactgtc tctgggcatg cgcctggaaa
    13981 actggccacc ggcaagcatc gctgacgagt aaccggatca caggttaagg ttttactgag
    14041 aaggataagg tcatgcgcca tcgtaagagt ggtcgtcaac tgaaccgcaa cagcagccat
    14101 cgccaggcta tgttccgcaa tatggcaggt tcactggttc gtcatgaaat catcaagacg
    14161 actctgccta aagcgaaaga gctgcgccgc gtagttgagc cgctgattac tcttgccaag
    14221 actgatagcg ttgctaatcg tcgtctggca ttcgcccgta ctcgtgataa cgagatcgtg
    14281 gcaaaactgt ttaacgaact gggcccgcgt ttcgcgagcc gtgccggtgg ttacactcgt
    14341 attctgaagt gtggcttccg tgcaggcgac aacgcgccga tggcttacat cgagctggtt
    14401 gatcgttcag agaaagcaga agctgctgca gagtaatctg aagcaacgta aaaaaacccg
    14461 ccccggcggg tttttttata cccgtagcat ccccacttat ctacaatagc tgtactcttt
    14521 ttgttcatcc cctggagtat ttatgtggtt acttgaccag tgggcagagc gtcatatagc
    14581 agaagcgcaa gcgaaaggtg agtttgataa tctggcaggt agcggcgaac cattgatact
    14641 ggatgatgat tctcatgtgc caccggaatt acgtgcgggg tatcgcttgc tgaagaatgc
    14701 tggttgctta ccgccagaac ttgagcagcg gagggaagca attcagcttc tggatatcct
    14761 gagaggtatc cgtcacgatg atccgcaata tcaagaggtt agccgtcgat tgtcattact
    14821 ggaattgaag ctgcgacaag ctggatttag taccgatttt ttacgcggcg attatgctga
    14881 caagttgttg gacaaaatca acgataacta gtggagtatg tatgtatcgc attggtgagc
    14941 tggcaaaaat ggcggaagta acacctgaca cgattcgtta ttacgaaaaa cagcagatga
    15001 tggagcatga agtgcgtact gaaggcgggt ttcgcctgta taccgaaagc gatctccagc
    15061 gattgaaatt tatccgccat gccagacaac taggtttcag tctggagtcg atccgcgagt
    15121 tgctgtcgat ccgcatcgat cctgaacacc atacctgtca ggagtcaaaa ggcattgtgc
    15181 aggaaagatt gcaggaagtc gaagcacgga tagccgagtt gcagagtatg cagcgttcct
    15241 tgcaacgcct taacgatgcc tgttgtggga ccgcccatag cagtgtttat tgttcgattc
    15301 ttgaagctct tgaacaaggg gcgagtggcg ttaagagtgg ctgttgattt tttgcactgg
    15361 caggattaca ctcgcgccgt aaaataacca attggagttt ttatgagccg atatcagcat
    15421 actaaagggc agataaggga taatgcgata gaagcattac tacatgatcc cttattccga
    15481 cagcgcgtag agaaaaataa gaaggggaaa ggcagttaca tgcgaaaagg aaaacatggc
    15541 aatcggggga actgggaggc cagtggcaag aaagtaaatc acttttttac cactggtctt
    15601 ctgctttcag gcgcttgtta agagcggtta ttctgctctt tcagcaaatc acgaatttct
    15661 gccagtaata cttcttcttt agttggtgca ggtgcggcag ctggttcttc ttttttccga
    15721 ttcagtttgt tgattagctt aatcgccata aagatggcaa aggccacaat cagaaaatca
    15781 aagacgtttt gaatgaagac accgtaatgc atcacaacag cagggatatc cccctgcgca
    15841 tcgcgtagcg tgacagcaaa ctgtttaaag tcgatcccgc caattaataa gcccagaggt
    15901 ggcatgatga tatcggcaac cagtgaagat acaatcttcc cgaatgccgc accgataatg
    15961 acacccaccg ccaaatccac cacgttcccg cgcatcgcaa attcgcgaaa ttctttaata
    16021 atgctcatgt tattctccct atgaagccga caaccataag tctaacaaat gttaagccat
    16081 tttcctgcca ggaatgaatt taattaatcc ttgataatca aagggaaata ataaggcgtc
    16141 attagacgcc ttattaatta caagaagaaa gggcttggct ggaagagtct ttcgacgtcg
    16201 gtaataaact ttttatctgt gaggaacata attacgtgat cgccttgctc aatgcgcaga
    16261 ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg ctccaataat cgttcctggc
    16321 ggtagcttga tttcgtcaat gactctgccg acaacgcgtg aggtgctttc atcaccgtga
    16381 gcaacggctt caatagcttc tgctacgccg cggcgcaatg aggaaacacc aacaatatct
    16441 gcttttcgca catggctaag caacgcagaa atagttgctt gttgtggtga aattgcaata
    16501 tcgatcacgc tcccctgaac cagatccaca taagcgcgac gctggatcaa taccatcacc
    16561 tttttcgcac ccatacgttt ggcaagcatg gcggacatga tattggcctc gtcatcgttg
    16621 gtgacggcaa taaacagatc aacttgatcg atatgttctt cggccagcag ttcttgatcc
    16681 gacgcatcac caaaaaagac gatcgtattc tgcaactttt ccgccagttc ggcagcgcgc
    16741 tgctgattac gttcgatgag tttaacgctg taatcttttt ccagacgacg cgccagccct
    16801 gcaccgatat taccgccacc aaccagcatg atccgcttat acggtttttc cagtcgctgt
    16861 aattcactca tcaccgcgcg gatatgctgt gaagcggcaa taaagaacac ttcatcacca
    16921 gcttcaacaa tggtcgaacc ttgtggacga atggggcgat cgtggcggaa aattgctgcc
    16981 acacgagtat cgatatgtgg catatgttcg cgcatggtcg aaagtgcatt accaatcagc
    17041 gggccgccat aataggcttt aactacagcg aggctgactt taccctcagc gaagttcacc
    17101 acctgcaatg cgcctggata ctcaatcagt cggtaaatat tatcgataac caactgctct
    17161 ggtgcgatca gatgatcaat cggtacagca tctgaatgaa atagcttatc ggcatcgcgt
    17221 acgtagtctg gtgagcggat gcgagcgatg cgattagggg tgttgaaaag tgagtaggct
    17281 acctggcagg caaccatatt ggtttcatct gaactggtta cagcaaccag catatcggcg
    17341 tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc catgcccctg cacgactcgc
    17401 aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt tggtatcgac aacagtaata
    17461 tcgttgttct cgccaaccag gttttccgcc agtgtgccgc caacctggcc ggcacccaga
    17521 atgataattt tcatcagtct cgacccgtta tctcatcact ttttgattag cttagcgtaa
    17581 aagaagccgt cgccctcttc ggcaccaggc agattttgtt tacccggttg ctctggtgtt
    17641 cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa aagctttaat ctgcaggcta
    17701 ttctcttccg gtaacaccga acaggtggca tagaccaggg ttccaccgga ttttaaatgc
    17761 ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga gttccgggat atcgcgatcg
    17821 cgacgtaacc atttaatatc cggatggtga cgaatcaccc ccgttgctga acaaggcgca
    17881 tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt gggaagggta acggccatca
    17941 ccttgtttca cggttgcctt cataccaagg cgttttaaat tgtcgtaaac gcgagagagg
    18001 cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg gtgccacctc aaggatatgc
    18061 gttgttttac cgccgggggc ggcacaaaga tccagaatgt gttcaccgtt ttgtggcgca
    18121 agccaggtca tgcaaccttg tgctgatgca tcctgaacgg taacccatcc gtcttcaaaa
    18181 ccaggtagcg catgaacagg tgcaggtgtt tccagacgta cagcatcagg gtaatccgca
    18241 tgcgggaaac ctttcattcc tgcttcatcc agcaatgcaa gccagctgtc gcgggaatga
    18301 tgcgtacgat taacgcgcag ccacattggc ggacgctggt tattggcttc gacgatggat
    18361 tgccactgct ctggatacgc tttttgcaga cgcttcagca accaggaagg atgcagataa
    18421 cgtgcatcac tggcattaaa cttggctaat aactcttctt gctgacgctg gaactggcgt
    18481 aatacaccgt taatcaaccc tttaagttgc ggacgcttaa ttgcgatagc gccttcaacc
    18541 gtttcagcca gcgcagcatg aggtggaatg cgggtataaa gcagttgata caaaccaacc
    18601 ataatcaggt aatgcacagt gcgctgtttg ccggtcatcg gacgggccat taacttatta
    18661 atcagccagt ctaactgcga aagcgtacgc agtacgccaa agcacaactc ttgaagaagt
    18721 gctttgtctt tatcagaaac tttttgctgg agcggtggca gaatgttgct taatgattgc
    18781 ccttgctcga cgacttgttc aacggcctgg gccgccatgc tacgtaaatt acgttgtttt
    18841 ttcataagta taaaaatgcc cggtaagacc gggcttagaa gagtggacta tcaggccaga
    18901 cggttgcccg gaacaaacca ttcccgacga gaattcagga ggtcttgcgc gctcatcgct
    18961 ttcttacccg caggttgtaa cgagagcaga ttcaggattc catcaccagt tgcaacctga
    19021 atgccttgtt tgttggcttc aaggatcgtt cctggtgcag cgttggttgc cgtatcaatg
    19081 accgacgctt tccagacttt aaccggctgt ctttcaattt ccagccagct cattggccat
    19141 ggattgaaag cgcgaatgta gcgttcaagc tgtgctgccg aaagtgacca gtcaatacgc
    19201 gcttcttctt tactcaactt ttcggcgtaa gtgacaagag tttcgtcctg aacttctggt
    19261 ttcgccgtgc cgtctgccag ttgtttcaac gtggtgataa gcccttgtgg gccaagctct
    19321 gccagcttgt cgtacagcgt accactggta tcttctgcag taatcgggca ggagagctta
    19381 tagagcatgt caccggtgtc taaaccgaca tccatttgca taatggtcac accagtttct
    19441 gcatcacccg cccatagtga gcgttggatt ggtgcagcac cgcgccagcg tggcagcagt
    19501 gaaccatgaa cgttgataca gccaagacgc ggcatctcca gcactgcttt aggcagaatt
    19561 aagccatagg cgacgacgac cataacatca gcctgcagat cggcgaccag ttgctggttt
    19621 tcttgtggac gcagggaaac aggttgaaaa acgggcagac ctttttcctc agccagaact
    19681 ttaaccgggc tgggcatcag ttttttaccg cgtcctgccg gtcggtctgg ctgggtgaac
    19741 acgccaacga cgttatgacc agaagacaac agcgcgtcga gatgacgcgc tgcaaagtca
    19801 ggtgtacccg caaaaataat acgtagtgat tctgacacgt tagttcttat ccttaagccc
    19861 gggctttcag acgatccagt ttttcaactt tctgacgaat acgttgttgt ttcaacggtg
    19921 acagataatc cataaacagt ttgccgacca ggtgatccat ctcatgctga atacagatgg
    19981 ctaacagacc gtctgcttcc aattcaaatg gtttaccgtc gcggtccagg gcgcgaattt
    20041 taactttctc tgcgcgtggc actaaagcac gttgttcagg gatcgacagg caaccttctt
    20101 caatgcctgt ttcgccgctt ttttctaaaa gctccggatt gattaacacc agccgttcgt
    20161 cacggttttc cgaaacatca ataacaatga tacgttgatg gatatcaacc tgggttgccg
    20221 ccaggccaat accttcttct gcgtacatcg tctcgaacat atcatcgacg atacgctgaa
    20281 tttctgcatt cacttcttct accggtttag caactttgcg aagccgctcg tccggaatat
    20341 gtaacacttg caaaactgac ataaatctcc agagatgtgt tcaggagtta gaaagattat
    20401 ttcttctatt ctagacaaat ccccctctga ttgacagcat cactgaccaa tcgcaaagat
    20461 tgctaaggct gcttatggca gggagataag gatggtcgat acagatattt ggctgcgttt
    20521 aatgagcatc agcagcttgt acggcgatga tatggtccgt atagctcact ggctggcaaa
    20581 acagtcgcat attgatgcgg ttgtattgca gcaaacaggg cttacattgc ggcaggcaca
    20641 acgctttctt tcatttccgc gaaagagtat cgaaagctca ctttgttggt tggagcaacc
    20701 caaccatcat ttaatccctg cggacagcga attttatcct cctcaacttc tggcgacgac
    20761 agattacccc ggcgcactgt ttgttgaagg agaactgtac gcgctgcatt catttcagct
    20821 tgccgtagtg gggagtcggg cgcattcatg gtatggcgag cgatggggac gattattttg
    20881 cgaaactctg gccacgcgtg gagtgacaat tacgagtgga ctggcgcgtg gaatcgatgg
    20941 tgtggcgcat aaagcggcct tacaggtaaa tggcgtcagc attgctgtat tggggaacgg
    21001 acttaatacc attcatccgc gccgccatgc ccgactggct accagtttgc ttgaacatgg
    21061 tggggcactt gtctcggaat ttcccctcga tgtttcaccc cttgcttaca atttcccacg
    21121 aagaaatcgc attatcagtg gtctaagtaa aggtgtactg gtggtggaag cggctttgcg
    21181 cagtggttcg ttggtgacag cacgttgtgc gcttgagcag gggcgtgaag tttttgcctt
    21241 gccaggacca atagggaatc cgggaagcga agggcctcac tggttaataa aacaaggtgc
    21301 gattcttgtg acggaaccgg aagaaattct ggaaaacttg caatttggat tgcactggtt
    21361 gccagacgcc cctgaaaatt cattttattc accagatcag caagacgtgg cattgccatt
    21421 tcctgagctc ctggctaacg taggagatga ggtaacacct gttgacgtcg tcgctgaacg
    21481 tgccggccaa cctgtgccag aggtagttac tcaactactc gaactggagt tagcaggatg
    21541 gatcgcagct gtacccggcg gctatgtccg attgaggagg gcatgccatg ttcgacgtac
    21601 taatgtattt gtttgaaacc tatattcaca cagaagctga gttgcgtgtg gatcaagaca
    21661 aacttgaaca ggatcttacc gacgcaggat ttgatcgaga agatatctac aatgccctgc
    21721 tttggctgga aaagcttgcg gattatcagg aaggactgac agaaccgatg caactggcct
    21781 ctgatccact ctccatgcgt atttataccc ctgaggagtg tgaaagactg gatgccagct
    21841 gtcgtggttt tctgcttttc cttgagcaaa ttcaggtgct taaccttgaa atccgtgaaa
    21901 tggtgataga gcgagtgctc gcgctggata ccgcagagtt cgacctggaa gacctgaaat
    21961 gggtaatcct gatggtgttg ttcaatattc cgggctgcga aaatgcgtac cagcaaatgg
    22021 aagaattact ctttgaagtg aatgaaggta tgctgcatta atcatttctt taattcagca
    22081 taagttgtta tggcgaaatc agcactgttc acggtgcgta ataatgagtc ctgcccaaag
    22141 tgcggggctg aactggttat tcgatccggg aaacacggtc cgtttcttgg atgctcacag
    22201 tatccggcgt gtgactacgt ccgtcctctg aaatcttcag cggatggaca tattgtcaaa
    22261 gttctggagg ggcaggtttg ccctgcatgt ggcgcaaatc tggtattacg ccagggacgc
    22321 tttggtatgt ttattggttg cagtaactac cctgaatgcg aacataccga acttatcgat
    22381 aaaccggacg aaacagcaat tacatgcccc caatgtcgga cgggccatct ggtccagcgc
    22441 cgctcccgtt atggcaaaac atttcactcc tgtgatcgct acccggagtg tcaatttgcc
    22501 attaacttca aacccatagc tggagaatgc cctgagtgtc attatccact actcatcgaa
    22561 aagaaaaccg cgcagggtgt aaaacacttt tgtgccagta aacaatgtgg aaagccggtt
    22621 tcggcggaat aataacgtga ataataacct gcaaggagac gctatcgcag ctgcgataga
    22681 tgtcctcaat gaagaacgtg tcatcgccta tccaacggaa gccgttttcg gtgttgggtg
    22741 cgatcctgat agcgaaacag cagtgatgag actgttggag ttaaaacagc gtccggttga
    22801 taaggggctg attttaatcg cggcaaatta cgagcagctt aaaccctata ttgatgacac
    22861 catgctgact gatgcgcagc gtgaaaccat tttttcccgc tggccaggtc ctgtcacctt
    22921 tgtctttccc gcgcctgcga caacaccgcg ctggttgacg ggccgctttg attcgcttgc
    22981 tgtacgagtc accgaccatc cgttggtggt tgctttgtgc caggcttatg gtaaaccgct
    23041 ggtttctacc agtgccaact tgagtggatt gccaccttgt cgaacagtag acgaagttcg
    23101 cgcacaattt ggcgcggcgt tcccggttgt gcttggtgaa acgggggggc gtttaaatcc
    23161 ttcagaaatc cgcgatgccc tgacgggtga actgtttcga caggggtaac ataatggaaa
    23221 cctatgctgt ttttggtaat ccgatagccc acagcaaatc gccattcatt catcagcaat
    23281 ttgctcagca actgaatatt gaacatccct atgggcgcgt gttggcaccc atcaatgatt
    23341 tcatcaacac actgaacgct ttctttagtg ctggtggtaa aggtgcgaat gtgacggtac
    23401 cttttaaaga agaggctttt gccagagcgg atgagcttac tgaacgggca gcgttggctg
    23461 gtgctgttaa taccctcaag cggttagaag atggacgctt gcagggtgac aataccgatg
    23521 gtataggttt gttaagcgat ctggaacgtc tgtcttttat ccgtccgggt ttacgtattc
    23581 tgcttatcgg cgctggtgga gcatctcgcg gcgtactact accactcctt tccctggact
    23641 gtgcggtgac aatcactaat cggacggtat cccgtgcgga agagttggct aaattgtttg
    23701 cgcacactgg cagtattcag gcgttgggta tggacgaact ggaaggtcat gagtttgatc
    23761 tcattattaa tgcaacatcc agtggcatca gtggtgatat tccggcgatc ccgtcatcgc
    23821 tcattcatct aggcatttat tgctatgaca tgttctatca gaaaggaaaa actccttttc
    23881 tggcatggtg tgagcagcga ggctcaaagc gtaatgctga tggtttagga atgctggtgg
    23941 cacaggcggc tcatgccttt cttctctggc acggtgttct gcctgacgta gaaccagtta
    24001 taaagcaatt gcaggaggaa ttgtccgcgt gaatcaggcc atccagtttc cggacaggga
    24061 agagtgggac gagaataaaa aatgtgtatg ttttcccgct ctcgtgaatg gtatgcaact
    24121 gacatgcgcg atctctggcg agagtctggc gtatcgcttt actggagata cgccagaaca
    24181 gtggttagcg agttttcgtc agcatcgctg ggacctggaa gaagaagcgg aaaacttaat
    24241 tcaggaacaa agtgaagatg atcaaggctg ggtctggtta ccctgatcca gatattcgtc
    24301 cttccatttc acgtaattat tcgcggaata gcgtaaccca gccttctctt catcacttaa
    24361 cgggcggatc tgtttgacgg ggctaccgag atacagatat ccgctctcca gccgtttatt
    24421 ttgtgggacc agactacccg caccaatcat cacatcatct tctactattg cgccatcaag
    24481 taaaattgag cccatcccaa ccaaaactcg attgccaatg gtgcagccgt ggagcatcac
    24541 cttgtgacca acagtgacat cttcgccaat ggttaatggg ttgccatctg ggttgtacga
    24601 ggatttatga gtgacatgca acatactgcc atcctggata ttggtgcgtg ctccgatctg
    24661 tacataatgt acatctccac gaatcacaac gagcggccag atccccacat catcagccag
    24721 acgaacgcca ccaatcacga cactgctatc gtcgatcatt acgcgctgac cgatttgtgg
    24781 aaaaagatcg cggtatgggc gtaaaacatc agacatactt acctcagcaa taaatgattt
    24841 actaatgact ttgggggcat tattggcctt gtgcaagtct tttagtatgc aaaaaagcac
    24901 cgttttgtgt gcgattgcag caaaaagggt gaaaaaacaa caaacagaaa aaaagatcaa
    24961 aaaaatactt gtgcaaaata ttgggatccc tataatgcgc ctccgttaag acgacaacgt
    25021 gaaacacttc acaggatggt cggaacaacg aagagaaaaa atcctgaaat tcagggttga
    25081 ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca gtgagctgaa agccgcgtcg
    25141 caactgctct ttaacaattt atcagacaat ctgtgtgggc actcgaagat acggattctt
    25201 aacgtcgcaa gacga
//
LOCUS       DAJTOR010000043        25163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTOR010000043 DAJTOR010000000
VERSION     DAJTOR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.043"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..184
                     /locus_tag="OO249_001596"
     CDS             <1..184
                     /locus_tag="OO249_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCR5635941.1"
                     /translation="ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPD
                     IGRQLSPDAWDDVSQE"
     gene            254..1030
                     /gene="yaaA"
                     /locus_tag="OO249_001597"
     CDS             254..1030
                     /gene="yaaA"
                     /locus_tag="OO249_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5635942.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1183..1479)
                     /locus_tag="OO249_001598"
     CDS             complement(1183..1479)
                     /locus_tag="OO249_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5635943.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGNRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(1693..2979)
                     /gene="thrC"
                     /locus_tag="OO249_001599"
     CDS             complement(1693..2979)
                     /gene="thrC"
                     /locus_tag="OO249_001599"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5635944.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(2980..3912)
                     /gene="thrB"
                     /locus_tag="OO249_001600"
     CDS             complement(2980..3912)
                     /gene="thrB"
                     /locus_tag="OO249_001600"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5635945.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(3914..6376)
                     /gene="thrA"
                     /locus_tag="OO249_001601"
     CDS             complement(3914..6376)
                     /gene="thrA"
                     /locus_tag="OO249_001601"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5635946.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6401..6520)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6456..6521)
                     /gene="thrL"
                     /locus_tag="OO249_001602"
     CDS             complement(6456..6521)
                     /gene="thrL"
                     /locus_tag="OO249_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5635947.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(6735..7421)
                     /locus_tag="OO249_001603"
     CDS             complement(6735..7421)
                     /locus_tag="OO249_001603"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5635948.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(7821..7961)
                     /locus_tag="OO249_001604"
     CDS             complement(7821..7961)
                     /locus_tag="OO249_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635949.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8057..8773
                     /gene="arcA"
                     /locus_tag="OO249_001605"
     CDS             8057..8773
                     /gene="arcA"
                     /locus_tag="OO249_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5635950.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(8833..10185)
                     /gene="creD"
                     /locus_tag="OO249_001606"
     CDS             complement(8833..10185)
                     /gene="creD"
                     /locus_tag="OO249_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5635951.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMPLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDWATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(10243..11667)
                     /gene="creC"
                     /locus_tag="OO249_001607"
     CDS             complement(10243..11667)
                     /gene="creC"
                     /locus_tag="OO249_001607"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5635952.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(11667..12356)
                     /gene="creB"
                     /locus_tag="OO249_001608"
     CDS             complement(11667..12356)
                     /gene="creB"
                     /locus_tag="OO249_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5635953.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12369..12842)
                     /gene="creA"
                     /locus_tag="OO249_001609"
     CDS             complement(12369..12842)
                     /gene="creA"
                     /locus_tag="OO249_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5635954.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13053..13922
                     /gene="robA"
                     /locus_tag="OO249_001610"
     CDS             13053..13922
                     /gene="robA"
                     /locus_tag="OO249_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5635955.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(13919..14566)
                     /gene="gpmB"
                     /locus_tag="OO249_001611"
     CDS             complement(13919..14566)
                     /gene="gpmB"
                     /locus_tag="OO249_001611"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5635956.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            14618..15139
                     /gene="yjjX"
                     /locus_tag="OO249_001612"
     CDS             14618..15139
                     /gene="yjjX"
                     /locus_tag="OO249_001612"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR5635957.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15224..15550)
                     /gene="trpR"
                     /locus_tag="OO249_001613"
     CDS             complement(15224..15550)
                     /gene="trpR"
                     /locus_tag="OO249_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5635958.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(15640..17577)
                     /gene="sltY"
                     /locus_tag="OO249_001614"
     CDS             complement(15640..17577)
                     /gene="sltY"
                     /locus_tag="OO249_001614"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5635959.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            17788..19455
                     /gene="ettA"
                     /locus_tag="OO249_001615"
     CDS             17788..19455
                     /gene="ettA"
                     /locus_tag="OO249_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5635960.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(19762..20994)
                     /gene="nadR"
                     /locus_tag="OO249_001616"
     CDS             complement(19762..20994)
                     /gene="nadR"
                     /locus_tag="OO249_001616"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5635961.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21015..22397)
                     /gene="radA"
                     /locus_tag="OO249_001617"
     CDS             complement(21015..22397)
                     /gene="radA"
                     /locus_tag="OO249_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5635962.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22446..23414)
                     /gene="serB"
                     /locus_tag="OO249_001618"
     CDS             complement(22446..23414)
                     /gene="serB"
                     /locus_tag="OO249_001618"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5635963.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23520..24164
                     /locus_tag="OO249_001619"
     CDS             23520..24164
                     /locus_tag="OO249_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5635964.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24192..>25163
                     /gene="lplA"
                     /locus_tag="OO249_001620"
     CDS             24192..>25163
                     /gene="lplA"
                     /locus_tag="OO249_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR5635965.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEK"
BASE COUNT         6019 a         6971 c         6247 g         5926 t
ORIGIN      
        1 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
       61 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
      121 gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga
      181 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
      241 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
      301 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc agttgatcca
      361 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
      421 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggcgaatgc
      481 ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacctt
      541 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
      601 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
      661 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
      721 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
      781 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
      841 gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg gtctgatgag
      901 tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
      961 aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga
     1021 gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     1081 agcatgcaga ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg
     1141 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
     1201 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
     1261 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
     1321 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgattgc ctatctgtaa
     1381 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
     1441 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
     1501 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
     1561 atcctaatct ctaagttacc gcatttattg agaatatctc ctttttccct gtcgtttttc
     1621 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
     1681 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
     1741 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
     1801 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
     1861 gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg ctacggcagc
     1921 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
     1981 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
     2041 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
     2101 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
     2161 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
     2221 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg
     2281 cagctgcgca actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
     2341 cgagttagct gagtttaacc ccagcgccac tttcagctct tcatcatcaa acgcctgctt
     2401 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
     2461 caatgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
     2521 cactttcaca ttcggtaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
     2581 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
     2641 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
     2701 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
     2761 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
     2821 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
     2881 gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
     2941 gacctgctca ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
     3001 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
     3061 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacacagag cgaacaaggt
     3121 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcaaccg cctgccgcgc
     3181 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
     3241 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
     3301 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc tggcttctgc
     3361 cgttgagact ttaatccccg gatacgccag cacccacagc cactcatcaa accctggcac
     3421 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
     3481 tgccacgttg tcgtaatgaa tgctgccgga gattcgtcct tccagctcgc ccatcaaagc
     3541 cagcaaacga gtgtcattaa gcggcttgcc acagtgttca ttcatcgcca tcagcgccgc
     3601 gaccaccgaa caggcgctgg agcctaagcc cgaaccgatt ggcatattct tttccagagt
     3661 catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc actgataaac
     3721 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact
     3781 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacaggtgt
     3841 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
     3901 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
     3961 caccggcagc tgtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
     4021 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
     4081 cattaccatc cacttcggca atcttcacgc ggcaggcacc atcttcatca atattgccaa
     4141 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
     4201 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcagcgtta aactctgcgg
     4261 gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga
     4321 gaatcaatag cttacgcgct acatccatac cagaaagatc atctcgcgga tccggttcgg
     4381 tataacccat ttcccgcgcc agagtagtcg cctcggagaa actcatgcct tcgtctaact
     4441 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcatc aattcatcac
     4501 cagcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
     4561 catagaggaa tttacgccgc gatttttccg ccgcatgacg caacagatgg tagtaatcca
     4621 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaagaagt
     4681 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
     4741 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgcg
     4801 ccagttcttc ctgccagttt tccagattta ggccatgcac attggtgagc agtgccttcg
     4861 agttggcaac accgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgttgac
     4921 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
     4981 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
     5041 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
     5101 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
     5161 taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta
     5221 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct cgcacacagt
     5281 cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
     5341 cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgccg accattcctt
     5401 tcatccccgg accagaaacg ctgaacattg ccatattatt cagattggaa atgcctttga
     5461 ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct tgaggatttc
     5521 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
     5581 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
     5641 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
     5701 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
     5761 cgttacgtcc aagtaccacc agttcgcctt tttcattacc ggaggtgaaa cctgccatca
     5821 acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac tcagcaatat
     5881 cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga tcgataacgg
     5941 taacgttgtg accacgagct tctaacacgc cggccataat ggcgatcgac attttctcgc
     6001 cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa ctaatgccat
     6061 gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat tgcgccagcg
     6121 ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
     6181 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
     6241 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
     6301 tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat gtaccgccga
     6361 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaag cccgcactgt
     6421 caggtgcggg cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta
     6481 atggtgatgg tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta
     6541 atttttatct gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa
     6601 taaaatttta atttactcac ggcaggtaac cagttcagaa actgctatca gacactcttt
     6661 ttttaatcca cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact
     6721 tactaaggcg ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac
     6781 attgccgtgt ctcgttgctc taaaagcccc aggcgttgtt gtaaccagtc gaccagtttt
     6841 atgtcatctg ccactgccag agtcgtcagc aatgccatga ctcgttcgcg taaagcttgc
     6901 agttgatgtt ggtctgccgt tgtgtcactt ttcgtcggtt gttgtattaa tgttgctaat
     6961 tgatagcaat agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc
     7021 ggcacaccag taagaacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg
     7081 cgaccaaaca ccagcgcggc atggctcatc catgaagatt tttcctctaa cagcagcacc
     7141 agttctactg gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcgaca
     7201 gtgaaatcga catcgtgtaa cgattcaacc agtgtcggga aaactttaat attatcaata
     7261 atatcaccag atccatgtgc gacccagcga gtggctggct ccagatgtgc ctgactatcg
     7321 acaatccgca gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt
     7381 tctgctctgg cgggtgcgac cagaataatc gttatacgca tattgccact cttcttgacc
     7441 aaatgaccgc gaacagggtg atcactgtca acttattaca cggtgcgaat ttacaaatcc
     7501 ttaacgtaag tcgcagaaaa agccctttac ttatcttaaa aaaggctaaa ctatttcctg
     7561 actgtactaa cggttgaatt gttaaaaaat gctacatatc cttctgttta cttaggataa
     7621 ttttataaaa aataaatctc gacaattgga ttcaccacgt ttattagttg tatgatgcaa
     7681 ctagttggat tattaaaata atgtgacgaa agctagcatt tagatacgat gatttcatca
     7741 aactgttaac gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt
     7801 gtccggcccc tcttagcctg ttatgttgct gttaaaatgg ttaggatgac agccgttttt
     7861 gacactgtcg ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg
     7921 atggaaagtg catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa
     7981 tttgcagcat gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc
     8041 aatttaggta gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac
     8101 acgcaacacg ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga
     8161 tggcgcggaa atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat
     8221 caatctgccg ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt
     8281 tgcgttgatg ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat
     8341 cggtgcagat gactacatca ccaaaccgtt caacccgcgt gaactgacga ttcgtgcacg
     8401 caacctgctg tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga
     8461 aagctacaag ttcaatggtt gggaactgga catcaacagc cgttcgttga tcggccctga
     8521 tggcgagcag tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa
     8581 cccaggcaaa attcagtctc gtgctgaact gctgaagaaa atgaccggcc gtgagctgaa
     8641 accacacgac cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac
     8701 gccggatacg ccggaaatca tcgccaccat ccacggtgaa ggttatcgct tctgtggtga
     8761 tctggaagat taatcggctt taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt
     8821 tatttttcaa ccttatttcc agatacgtaa ttgatcgtcc attgtaactt ctttactggc
     8881 tttcattttc ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa acatcatgcc
     8941 ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg cagagttgag
     9001 cagtccccac atcacaccat ccagcaacaa cagcgcgagg gtaaacaaca tgctgttgcg
     9061 ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc
     9121 gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa
     9181 catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag
     9241 cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt
     9301 cgcccagtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcggg
     9361 gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa
     9421 ccagctgctt tgccattgcg cctgaaaacc tgactcgcta acttcccgtt tggctggtag
     9481 aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aagggcattt cgctattacg
     9541 cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca tattcagctg
     9601 caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg
     9661 ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag gcgctttcac
     9721 cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc ctaagatgat
     9781 atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc
     9841 actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc
     9901 atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt
     9961 tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa gcggcccaac
    10021 gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc
    10081 ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca gcaatactgc
    10141 accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct tctcctttgc
    10201 aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg
    10261 aagtcgaagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt
    10321 aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt
    10381 tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc
    10441 actaccggta tccagcacct taagggcgac gtgttcctga tccacttcgg cgcttagcgt
    10501 tatgcgaccg ctctcggggg taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg
    10561 ctccagtaac gccggttcag cagcaacatt aacctcagtg ggcataacat gtagagtgat
    10621 gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata atgccgccac
    10681 atcaacaaca gtcagaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt
    10741 ttctaccaat gcctgcatac gcgcattttg cgtcagaatg ttgtcggtaa aacgagccac
    10801 cacttccggt ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg
    10861 gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag
    10921 cttcacgcgc atactttcca gcgcctgcgc aagcttacgc aactcgctac tgccgagttc
    10981 aggaagagga acgggcttat tgtcagtgac ggaatcggca tagcgagtga gccgggcaat
    11041 ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc ccagcaaaat
    11101 ggcgctggcc cataaaattc gccgctcgct acgcttaatg accggagcca tcgccgcgtt
    11161 cggtttgcct acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac
    11221 atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg caccatactg
    11281 accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgcccaa cggctttatt
    11341 tgccgaatcg aacaatactt taccctgcga atcggtcata tagacatgat attcattgcg
    11401 cactttgtta atgccaccga tattggcgcg aaacggacga tgttgtagct gattaaacgc
    11461 ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc
    11521 cagcaacgtt gcggtgtcga tcaacgtccc ctccgttgct cttcgcacgc ccggtttaac
    11581 ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc
    11641 cagcagcaac cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga
    11701 tgagtattaa tcggtgaaag atcggggttg atggcacgca gcttggcatg caacgtttta
    11761 atgtgggtat caacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatc
    11821 agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac
    11881 tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc
    11941 aattcaaaat gaccaatacg gatgacggga gacggcgacg agaacttctt cacccgacgc
    12001 agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg
    12061 tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac
    12121 agtacaggta acgccggatg gagcgccagt aactggctgc acaattcaaa gccgctgata
    12181 tcaggcaaac caacgtcgag aatcatgacg tcgggaacca gctggcgagc tttatccagc
    12241 accggcaagc ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc
    12301 agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgtataaat
    12361 cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa
    12421 ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa
    12481 aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg
    12541 ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa
    12601 gaaatagccg catcggaggt atcttccgcc agacccaatc cccctttaat accacctgtt
    12661 ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca
    12721 acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct
    12781 gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc
    12841 atattgttac catttttatc tattacacga gcaattagtt cgtcacggta aataacatat
    12901 tgattaagaa caaaatctca attcttttat ttccgtcaag ccctacaaca tactctacta
    12961 agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca
    13021 ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc
    13081 ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga
    13141 aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta
    13201 ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg
    13261 cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc
    13321 gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga
    13381 gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat
    13441 ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg
    13501 agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca
    13561 acgcgccgac cattccgcca gtgctctacg gcctgaatga aacgcgtccg agtcaggata
    13621 aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct
    13681 atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg
    13741 aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa
    13801 tgctcaacct gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg
    13861 ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt
    13921 aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa
    13981 cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt
    14041 tacgcagacg taagcggcgc gctgcccatg ctggtaatcc gagaatcgta ctcaccagac
    14101 atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc
    14161 aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa
    14221 tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca
    14281 gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa
    14341 agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta
    14401 aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg
    14461 cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg
    14521 cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta
    14581 tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg tctgtgcgac
    14641 caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg
    14701 atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag
    14761 tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga
    14821 ggctgatttt tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt
    14881 ggtgattgaa aacaccagcc agcgcggcga ggcgcgttcg gctactttgc cgttacctgc
    14941 ggtgattctg cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt cgcgttatac
    15001 cggtattgat gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact
    15061 cactcgcgcc agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc
    15121 cgtgtatcag gcattgtagg acggataagg cgttcacgcc gcatccggca ttcggtgcac
    15181 gatgcctgat gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca
    15241 acacctcttc cagccactgg cgcagctcca cgggcgcggc tttcaggctg ttagatccac
    15301 gcgtaatcgt cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc
    15361 cacgcaacag ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg
    15421 tcagcatcag gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt
    15481 cgacaaaacg taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt
    15541 gttgggccat aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccggttgta
    15601 ctcgctaaag agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc
    15661 cgtggcgctc atcaacgtcg gtttatcccc catgaaataa cggtagtaag cgtcataagc
    15721 cagcacgttc ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac
    15781 agcgtcgata cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt
    15841 ataagctgct gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta
    15901 actggtgcca atgttgatat tcgtttccgg atccagcaat tgcccagggc tgctataacc
    15961 aggaatagag aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc
    16021 gctggcccct accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc
    16081 catcgcatag ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta
    16141 agccagcggg aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac
    16201 gctaagatcc caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt
    16261 tgacttgctc ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta
    16321 catcaactcg cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg
    16381 cggcgcttta tcaatcttca gctcatactc ttctccgatg cgttgtgctg caaccatcgg
    16441 gtagaaacca cgctgttgca tgagttgatg caaaatctct ttcgcttcag cttcacgtcc
    16501 gcgttccagc aataaatccg cctgccagta acgccattca tctttctctt ttgcttccat
    16561 cggcagacgc gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gtgccattcg
    16621 cacacggcgt tcaatcaacg acgtggattg cgagcgcata atggcgtcat cgcgccattt
    16681 cgcctgctcg tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc
    16741 ctgaatctga tcttcattaa gctgctgcgc ctgggcaagc gacgggatca tcagccgtgc
    16801 attttcagca tcctgccgcg ccacactggc aaacgccacc gctgccattt gacgggtaaa
    16861 atcggtcgct ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga
    16921 aatgattgcc gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac
    16981 caggccagtg ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc
    17041 ttgtttacct gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt
    17101 gccggttagc cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca
    17161 tttcgcatag tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc
    17221 taacaagcca cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt
    17281 gcgagcggga ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg
    17341 attcatcaga tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt
    17401 cagtccaggc atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat
    17461 ttgcgcgtaa cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt
    17521 cagcagacag acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag
    17581 cgcatcctct aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta
    17641 accagtcatt gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga
    17701 tcgaccaact gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc
    17761 acgataaaca tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc
    17821 ggcaaagttg ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct
    17881 ggggcaaaaa ttggtgtcct gggtctgaac ggcgcgggta agtctaccct gctgcgcatt
    17941 atggcaggca ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt
    18001 ggttacctgc cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa
    18061 gaagcggttt ctgaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac
    18121 gccgatccgg atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc
    18181 attcaggctc acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgcta
    18241 cgtctgccgg actgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgta
    18301 gcgttgtgcc gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac
    18361 cacctggatg ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgagggcacc
    18421 gtggtggcga ttacccacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa
    18481 cttgaccgcg gtgaaggtat tccgtgggaa ggcaactact cctcctggct ggagcagaaa
    18541 gatcagcgcc tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa
    18601 gagctggagt gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg
    18661 gcacgctttg aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac caacgaactg
    18721 tttattccac ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa
    18781 tcctacggtg atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcaatc
    18841 gtcgggatca tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctctggt
    18901 caggaacagc cggacagcgg caccatcact ttaggtgaaa cggtgaaact ggcatcggtt
    18961 gatcagttcc gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg
    19021 ctggatatta tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt
    19081 aactttaaag gggttgatca gggtaaacgc gttggtgaac tttccggtgg tgagcgcggt
    19141 cgtctgcatc tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca
    19201 accaacgacc tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg
    19261 ggctgtgcga tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc
    19321 ctggactacc aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa
    19381 gagtacaaga aacgcacgct gggcgcagac gcactggagc cgaagcgtat caagtacaag
    19441 cgtattgcga agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat ccggcctaca
    19501 aaccaagaaa tttcaatacg ttgtgaatct ccaggcctga taagacgcgg taagcgtcgc
    19561 atcaggcatt gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg cctacaatag
    19621 ccttagtatc cgcaatttcg tagcgggtca cccgtgctga ttcttttaaa ccttgtttaa
    19681 tctcgacagc ctcttccaga gaggttttca actcatcaaa aaacttattt tatacctcgc
    19741 ctttgagccg tttcatcgcg gttatctctg ctcccccatc atctcccgca ccagctccac
    19801 gcagcgcagg aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt cgatattgtt
    19861 ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag
    19921 gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa
    19981 acggtattca tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt actttttgca
    20041 gaacgcctgg gtagtgacaa aatcagtgtc aataaacgcc actttattgg catatttcac
    20101 tgcaaaatca atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg aatactgcaa
    20161 tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt cccacgcact
    20221 ggtggtattg aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc
    20281 accaaggatc gccacggtac gtacaaagaa cggcttcact tccgtaggaa tatattccca
    20341 gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg
    20401 atcgaccagc accgtatcga tccccagatg ttccatatac tgtggcgcat cggcttcttc
    20461 cgaggtgtag atcaggtccg gctgaatccc cttttcagcc ataaactttt tgatgccgtt
    20521 gctccacaca tcccagccgt gcggatacgg ctccatgccc tcctcgttga aagcatgaat
    20581 gcgaatattt ttctgatatt taaaagtttg taataaccag cgcagacgat ccggcaccgt
    20641 gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc
    20701 cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc
    20761 ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct gtcggggaaa
    20821 ttcaagcccc aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc ttttaatttt
    20881 ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg
    20941 tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc
    21001 ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg
    21061 ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc
    21121 cggcgaaaac cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg caccgggcgg
    21181 atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca
    21241 cgcaggctgg aaaccatcgc cagcagtaac gctaaatcgg cactggtttc ggttaccttc
    21301 acgccgccga ccacgttcac aaatacatcc tgatcggcca tttgcagacc accgtgacgg
    21361 tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc
    21421 ggattcgcca tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt
    21481 ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa
    21541 attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc
    21601 tcattcaccg cgccgaagcg gtttttatgg ctgcgcaagg tgcgaaaacg ggagtcggca
    21661 tcaccatcca gaagcaccga acagtcgata cagtgttcca gcactttagg gccagccagc
    21721 gagccatctt tggttacgtg cccaaccatg acaatcgcca caccgcgcgt tttggcgaag
    21781 cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca
    21841 tccgccatat gcatcacctg gatcgagtca attaccatca gtttcggttg ctcttcttcg
    21901 gcaatcaggc agatctgttc gatgctggtt tccgacaaca tattgagatt gtcagtcggc
    21961 aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc agtgacatac
    22021 agcgttttca tctgctgggc cagtttgcac agcgtttgca gtagcagcgt ggatttcccc
    22081 gcacctgggt taccgccaat cagaatggcg cttcccggca ccacgccgcc gcctagtacg
    22141 cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag
    22201 agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc
    22261 gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca
    22321 ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt
    22381 tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atggcgggcg
    22441 agcaattact tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg
    22501 tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc
    22561 agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc
    22621 gccagcggga tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt gtactgcgcg
    22681 tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg
    22741 gctaccacgg cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa agtaaagccg
    22801 ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag ttgcgttaag
    22861 cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc
    22921 agcgtcgcca cacggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt
    22981 tcggttactt ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc atcaatacat
    23041 tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc
    23101 gggattttcc ccagcggggc gacatccagt tgcgcttcat gggccaggcg cgtagcccgt
    23161 gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca
    23221 atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca
    23281 cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca
    23341 ccacttaatg aaagaggcag accaggccat aaagagacat cttcaggcag gtcgcaccag
    23401 gtaatgttag gcattaagga tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag
    23461 gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga
    23521 tggctcgcac aaaactgaaa ttccggctgc atcgggcagt gattgtcctg ttctgtcttg
    23581 ccttgttagt ggcgctgatg cagggagcgt catggtttag tcaaaaccac cagcgacaac
    23641 gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtaacg ctgaacgttg
    23701 caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc ctcgatcagt
    23761 taacggatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg
    23821 cacgttctgg cgaaagcgtc gaagtgcgcg accggctggc gctcgacggt aaaaaagcgg
    23881 gcggctattt taaccagcag attgtcgaac caattgccgg gaaaaacgga ccgctcggct
    23941 atctgcgcct gacgctcgac acccatacgc tcgccaccga agcccaacag gtggataaca
    24001 ccactaacat tttacgcctg atgttgctgc tttcactggc aatcggcgta gtgctgaccc
    24061 gcacgctgct acagggtaaa cgtacccgct ggcagcaatc gcccttcctg ttaaccgcca
    24121 gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa
    24181 aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta
    24241 acctggcggt ggaggagtgt atttttcgcc agatgcccgc cacgcagcgc gttctgtttc
    24301 tctggcgcaa tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg aaagagtgta
    24361 atacccggcg gatggaagaa gataacgtcc gcctggcacg gcgcagtagc ggtggcggcg
    24421 cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg
    24481 ataaaaccat ctccacgtcg attgtgctca atgcgctgaa cgcgcttggc gtcagcgccg
    24541 aagcgtccgg gcgtaacgat ctggtggtga aaaccgccga aggcgaccgc aaagtctcag
    24601 gatcggccta tcgcgaaacc aaagatcgtg gcttccacca cggcaccttg ctgctcaatg
    24661 ccgaccttag ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag
    24721 gcattacctc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggatcaccc
    24781 atgagcaggt ttgcgaggcc ataaccaagg cctttttcgc ccattatggc gagcgtgtag
    24841 aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaatctttg
    24901 cccgtcagag tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg
    24961 atgaacgctt tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
    25021 cccgcgccca ggtgtttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc
    25081 gactgcaagg ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg
    25141 ttgacttccc ggaccaggaa aaa
//
LOCUS       DAJTOR010000044        24877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTOR010000044 DAJTOR010000000
VERSION     DAJTOR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.044"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..337)
                     /locus_tag="OO249_001621"
     CDS             complement(<1..337)
                     /locus_tag="OO249_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635966.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAA"
     gene            complement(453..1004)
                     /gene="maa"
                     /locus_tag="OO249_001622"
     CDS             complement(453..1004)
                     /gene="maa"
                     /locus_tag="OO249_001622"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR5635967.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(1176..1394)
                     /gene="hha"
                     /locus_tag="OO249_001623"
     CDS             complement(1176..1394)
                     /gene="hha"
                     /locus_tag="OO249_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5635968.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1420..1794)
                     /gene="tomB"
                     /locus_tag="OO249_001624"
     CDS             complement(1420..1794)
                     /gene="tomB"
                     /locus_tag="OO249_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5635969.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2340..5489)
                     /locus_tag="OO249_001625"
     CDS             complement(2340..5489)
                     /locus_tag="OO249_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5635970.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5512..6705)
                     /gene="acrA"
                     /locus_tag="OO249_001626"
     CDS             complement(5512..6705)
                     /gene="acrA"
                     /locus_tag="OO249_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5635971.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDNKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            6847..7494
                     /gene="acrR"
                     /locus_tag="OO249_001627"
     CDS             6847..7494
                     /gene="acrR"
                     /locus_tag="OO249_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5635972.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7622..10984
                     /gene="mscK"
                     /locus_tag="OO249_001628"
     CDS             7622..10984
                     /gene="mscK"
                     /locus_tag="OO249_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5635973.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLELQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIVGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYIDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11196..11357)
                     /gene="rsmS"
                     /locus_tag="OO249_001629"
     CDS             complement(11196..11357)
                     /gene="rsmS"
                     /locus_tag="OO249_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5635974.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11371..11898)
                     /gene="priC"
                     /locus_tag="OO249_001630"
     CDS             complement(11371..11898)
                     /gene="priC"
                     /locus_tag="OO249_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5635975.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            11968..12345
                     /locus_tag="OO249_001631"
     CDS             11968..12345
                     /locus_tag="OO249_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5635976.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12498..13049
                     /gene="apt"
                     /locus_tag="OO249_001632"
     CDS             12498..13049
                     /gene="apt"
                     /locus_tag="OO249_001632"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5635977.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13178..15049
                     /gene="dnaX"
                     /locus_tag="OO249_001633"
     CDS             13178..15049
                     /gene="dnaX"
                     /locus_tag="OO249_001633"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5635978.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVPSALEKAP
                     AKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAARVS
                     QLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSTLKGSTVEL
                     TIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI"
     misc_feature    14442..14506
                     /gene="dnaX"
                     /locus_tag="OO249_001633"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            15102..15431
                     /locus_tag="OO249_001634"
     CDS             15102..15431
                     /locus_tag="OO249_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5635979.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15431..16036
                     /gene="recR"
                     /locus_tag="OO249_001635"
     CDS             15431..16036
                     /gene="recR"
                     /locus_tag="OO249_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5635980.1"
                     /translation="MQTSPLLTQLMEALRCLSGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16146..18020
                     /gene="htpG"
                     /locus_tag="OO249_001636"
     CDS             16146..18020
                     /gene="htpG"
                     /locus_tag="OO249_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5635981.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18201..18845
                     /gene="adk"
                     /locus_tag="OO249_001637"
     CDS             18201..18845
                     /gene="adk"
                     /locus_tag="OO249_001637"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5635982.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            18977..19939
                     /gene="hemH"
                     /locus_tag="OO249_001638"
     CDS             18977..19939
                     /gene="hemH"
                     /locus_tag="OO249_001638"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5635983.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEYIEMMANLVAAYR"
     gene            complement(19936..20895)
                     /gene="aes"
                     /locus_tag="OO249_001639"
     CDS             complement(19936..20895)
                     /gene="aes"
                     /locus_tag="OO249_001639"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR5635984.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            21047..22351
                     /gene="gsk"
                     /locus_tag="OO249_001640"
     CDS             21047..22351
                     /gene="gsk"
                     /locus_tag="OO249_001640"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5635985.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22484..24160)
                     /locus_tag="OO249_001641"
     CDS             complement(22484..24160)
                     /locus_tag="OO249_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5635986.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVVSARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(24398..>24877)
                     /gene="fsr"
                     /locus_tag="OO249_001642"
     CDS             complement(24398..>24877)
                     /gene="fsr"
                     /locus_tag="OO249_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5635987.1"
                     /translation="SYYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIG
                     RKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIG
                     MVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         6216 a         6484 c         6389 g         5788 t
ORIGIN      
        1 gcgcggcagg tacaaagcgc gtgttgtacc attcgccgtt atagcaaacg cggaaatcca
       61 gcacgtcgat atcttcatag cggtagcgtg ggtagacatc gaaaaagaag aaaccattaa
      121 gcaggataaa taatacaacc agtagccaga cagagcctga cagcgtttcg gactgcaaca
      181 tcaccgccag ggtggcaaaa aaagcaacgt acataccgat aaacagcccc ggatgtttac
      241 ggataaaact gatactaaag cggggtttgt tgtcgcgttt ttcgcgggta ttcagagact
      301 caatcgtttc ggtcagcagg cgttgtattt cggtcatcgt ttgccttcgt ggtatctgca
      361 actttccagg gaacacccta ttttaacgtg ggaatagtag aggggaagta acagatctac
      421 cacaattttg cataacagtt gcgaaaaacc gattacaatt ttttaattat tctggctgga
      481 ttaccgccca cgacaacgtt gtccgggaca ccttttgtga caactgcacc tgaagctacc
      541 acgacgttat caccaatggt cacaccaggg ttaatgaccg cgcgtccgcc aatccagacg
      601 ttattaccga tggtgacggg tttccccagt tcagcaccgc tattacgtgc tacagggtcg
      661 atgggatgtg ttgccgtgta gatatgaacg cctggtgcca acatacagtt atcaccgatg
      721 cgaatagggc agacatcaag catcacgcaa tcgaagttgg cgaaaaaatt attgccgaga
      781 aaaatgttat agccataatc acagcgaaac gttggctcaa tataagcctc tgtcacctga
      841 ccgaataaat cagcgagaat ttgctggcgt aatgtgtgct cttccgccag ggaatgattg
      901 tatcggtgaa taagctgacg agcgcgcagg cgatcgcgag ataacgtctc atctgccgag
      961 cgatacaact caccagcaat catcttttct ttttctgtgc tcattactga cctccggttg
     1021 ctgaaaatgc ggcaacacag taagacgaaa agtcagtgaa cagaacgttc ccattacatc
     1081 tgtgtgataa agatcacata gggtatgtct tcatggtgaa aaagtataaa attcttaata
     1141 aacagccggt tatagctccg aaagcgaatt atttattagc gaataaattt ccatactgag
     1201 gaagggatct tgtcgtatag tttattcatg gtcaattcgg cgaggcggtg atctgcggct
     1261 gagtaaaata ccgccagttc attatctgat aattcgtatt tatttttctc gataacacgc
     1321 tccagcgtgt caattgtctg gcaacgacgt aaacgcatta aataatcggt tttcgttaaa
     1381 ggtttttcgg acatacttct acccatggtt gtaataattc taacaagata aactcgcagg
     1441 attctctttc gtcgcattga caaaacaacg gaatagtcga ttacctgact tccgccattt
     1501 ctgaagatcc tgcatattaa taccataact actgaacaac ataaaggtgt catccagata
     1561 ttcgtcgatc tgctcaatga gcttattgtc ttcattatac ttaattttgt aattaagtgc
     1621 gaaggtcgca atatgctcaa tcagttcatt caactggagg ttgatcgccg aggttgggtc
     1681 gttaacccag ccataattgc tttcttcaag gtttgcaagg cagtcatgat acagggtttc
     1741 acagagaaac ttaagctgtg cgatatcatg tcttttgggt gagtattcat ccataacgcg
     1801 tccccttctt agcggttgaa ctaacggaca cctttcggga tggaaaaaac ttactgacct
     1861 ggacttgccc tctcgtttgt tagcttaact ataagccact ctttgcaggt attcatcgca
     1921 ttattacgaa aaattacaat taggaaggaa tcgggacagg actcactttt tcatactagc
     1981 actaacccgc agcaggtaaa agcagtttgc tcgaactgtt cataacattt caggattatc
     2041 gccagatgaa attggtgaca ctaataccag gattgctctg aatatgacgt aataaccgag
     2101 gaatgaataa agaattaccg caaataatta agaatagcct ctaaatgatt atggattatt
     2161 aaatctatta gcgctactta atataaatta actaacaatc agtatttttt atgaatttta
     2221 tccgtggtta atactggttt tcgtatgaga tcctgagttg gtggttcaat tactccttaa
     2281 tgttcgtagg ttatgcataa aaaaggccgc ttgcgcggcc ttagtgatta cacgttgtat
     2341 caatgatgat cgacagtatg gctgtgctcg atatcttcat tcttgcggct aaagcggcgg
     2401 cgaaccacca caaagaatac cggaacgaag aagattgcca gtaccgttgc ggtcaccatc
     2461 ccgcccatta caccggtacc tactgcgttc tgcgcgccgg aaccagcacc agtactgata
     2521 accagcggca taacgccgag gataaacgcc agcgaggtca tcaggatcgg acgtaaacgc
     2581 atccgcactg catcaagcgt cgcttcaatc agacctttac cttctttatc catcaagtct
     2641 ttggcgaatt cgacgataag tatcgcgttc ttcgccgaca acccaatggt tgtgagcagg
     2701 cctacttgga agtaaacgtc attggtcagg ccacggaagg tggcagccag caacgcaccg
     2761 ataaccccca gcggaacgac cagcataacg gagaacggaa tcgaccagct ctcgtacagc
     2821 gccgctagac acaggaatac gacaatcaac gaaatcgcgt acagtgaagg tgcctggttg
     2881 ccggagaggc gttcctgata ggacatcccc gtccagtcat agccaacacc ggtaggcagt
     2941 ttgctcgcca gttgttccat tagctccatt gcttcaccgg tacttttacc cggtgccgcc
     3001 tggcctaaga tttccatgga tggcaggcca ttgtaacgtt ccagacgcgg cgaaccgtac
     3061 tcccaacgag aagaggagaa tgccgagaac ggcaccatct gaccatcagc agcacgaaca
     3121 taccagtcgc cgatatcatc cggcagcata cggtatttcg cttctgacat gacgtaaact
     3181 ttcttcacac gaccgcggtc gataaagtcg ttcacatagc taccgcccca tgcagcgccc
     3241 agagtggtgt taatgtcgtt gatagaaaca cccagcgcct gcgctttttc ctggtcgata
     3301 tcaatcttaa actgcggggt atcttccaga ccgtttggac gtacgctggt caacatatca
     3361 ggatgctttg ctgcttctgc aagcagctgg ttacgcgcct gagtcagttt ttcgtgacca
     3421 aggccagcct ggtcaatcag ctcaaagtca aagccggttg cagtacccag ttccacgatt
     3481 gcgggcaggt taaaggcgaa aaccatcgca tctttgattt gcgagaaagc gcgtgttgca
     3541 cgcatggtaa tcgcttcaac tttgttttct tcgcccggac gatcggccca gtccttcaag
     3601 gaaacgaacg caataccggt attctgacca cgtcccgcaa agccgaagcc gttaacggcg
     3661 aacaccgact caacgttgtt cttttctttg gtcagatagt aatgcgttac ctcattgagc
     3721 actttctgcg tacgttcctg cgttgcacct gctggcagct gaaccatggt cataaatacg
     3781 ccctggtcct catctggcaa gaaggagctt ggcagacgca cgaacagata ggccatgccg
     3841 accacgatga tcagatacag caccaggtaa cgccccgtac tgcgcagaat accgcctacg
     3901 ctgtcggtgt agtggtgcgt gctcttctcg aacatgcggt taaaccagcc gaagaagcct
     3961 tttttacctt ccccgtgatc gcctttggca atcggtttca gcatggtggc acaaagagcc
     4021 ggagtcagga tcaacgctac cagtaccgac agcgccattg ctgaaacaat ggtaatagag
     4081 aactgacgat agatagcacc agtagaaccg ccaaagaagg ccatcggtac gaataccgct
     4141 gacagtacca tcgcgatacc gaccagagcg ccctgaatct gccccatcga cttacgggta
     4201 gcttcttttg gcggcaaacc ttcttccgcc ataacacgct ccacgttttc taccacaacg
     4261 atagcgtcat ccaccaacaa gccgatggcg agcaccatcc cgaacattgt tagcgtgttt
     4321 atcgagaagc caaaggcggc aaggacggca aaggtcccga gcaataccac cggtacggca
     4381 atggtcggaa tcaacgtcgc gcggaagttc tgcaggaaca gatacataac caggaacacg
     4441 aggatgatcg cttcgaccag cgttttaacc acttcgtgaa tagagatttt cacgaacggc
     4501 gtggtgtcgt acgggtaaac aattttcaga cccgacggga agaacggttc catcttcgcc
     4561 agttcagcac ggattgccgc agcggtatcc agcgcatttg caccggtcgc cagcttgatc
     4621 cccagaccgg aagccggttg gccgttaaac tctgcgatga tgtcgtagtt ctcaccaccc
     4681 agctcaatct tcgccacatc acgcagcagg acgcgggaac catcctgatt cactttcagc
     4741 aggattttgc cgaactcttc agtagaggtc agacgcgtct gagcaataat agaggcgtta
     4801 agctgttggc ctttcaccgg cggcgtacca ccgagctgac ccgccgcaac ctgggcgttc
     4861 tgcgctttga tggcggtaat gacatcaacc ggcgttagct ggaatttgtt cagctcattc
     4921 gggttcatcc agatacgcat cgcgtactgt gaaccgaaca actgaacatc acccacgccc
     4981 gacgtacggc tgatggcatc tttcatattc gccgctacgt agtcagagat atcctcctgc
     5041 gtcatggtgc catcggtgtt gataacgccg acaaccatca ggaagctgct ggatgatttc
     5101 tcaacgctca ccccttgctg ctgaacttct tgcggcagca acggcatcgc cagctgcagt
     5161 ttgttctgca cctgaacctg cgcgatatcc gcatcagtac cagactcaaa ggtcagggtg
     5221 atctgcacgg tccccgtgga gtcactgtta gaggacatgt acatcaggtt atcgataccg
     5281 ttcatattct gttcgataac ctgtgtcacc gtgtcctgca ctgttttcgc atcagcgccg
     5341 gggtaggagg cggagatcgt tactgccggc ggtgcaatcg taggatattg cgccaccggc
     5401 agtttgagga tcgccagccc ccctgccaac atgatgataa tggcgatcac ccacgcaaaa
     5461 atcgggcgat cgataaagaa attaggcatg tcttaacggc tcctgtttaa gttaagactt
     5521 ggactgttca ggctgagcac cgcttgcggc ttgctggtta ttatcagcgg taacttcttg
     5581 tgcttttacc tggacaccag gacgcacttt ctgcagccca cttattacta cgcgatcgcc
     5641 tgctttcaga ccttctgtca ccagccactt atcgccaata gcctggcttg caacgatcgg
     5701 acgggtttcc actttgtcat ccgcgccaac taccagtacg gtggcatcac cacgcggcgt
     5761 acgggttacg ccctgttgcg ggactaaaat agcgtttgga ttaagccctt cttccagacg
     5821 tgcgcgcacg aacatacccg gcagcagagt gtgatccggg ttcgggaaga tagcgcgtag
     5881 ggtgatagac ccagtggtct gatcaacggt aacgtcagag aattccagcg taccgtcctg
     5941 cgggaactta atgccgtcac tggtgatcag cgacactttg gctttgccgt tctcttgttt
     6001 cagcgtgcca ttcgccagtt cctgtttcag gcgcaggaag tcgttgctgg actgggtcac
     6061 atcaacgtag atcggatcaa gttgctgcac ggttgccagc gcagtcgcct gaccgttctg
     6121 taccaatgcg ccttccgtca cgttcgactt accaatgcga ccgctaatcg gagcggtgac
     6181 tttggtgtaa gccagattga tccgcgcagt ttcaacggca gctttcgccg cagttaccgc
     6241 agcattcgcc tgttgcgcat cagccagagc ctgatcgtac tcttgcttac tgatgtactg
     6301 agtaccgagc agtttctgat aacgattcac cgtcaattgc gcgatattgg ctgcagcctg
     6361 ggctttcgcc agatcacctt tcgcactgtc gtatgtcgcc tgataggtcg caggatcaat
     6421 ctgatagaga gagacaccag cttcgatgtc gctaccttct ttgaaattac gcttcaggat
     6481 aatcccgcta acttgaggac gaacttctgc gatccggtag gcactggtgc gacccggaag
     6541 ctcggttgtg atctgcagag gttcagtttt gactgttact acgccaacgg cgggcatctg
     6601 ctggccacct tgttgggcct gtttgttgtc acatcctgtt agggctaagc tgcctgagag
     6661 catcagaacg accgccagag gcgtaaaccc tctgtttttg ttcatatgta aacctcgagt
     6721 gtccgatttc aaattggtca atggtcaaaa gttaataaac ccattgctgc gtttatatta
     6781 tcgtcgtgct atggtacata cattcacaaa tgtatgtaaa tctaacgcct gtaaattcac
     6841 gaacatatgg cacgaaaaac caaacaagaa gcgcaagaaa cgcgccaaca catcctcgat
     6901 gtggctctac gtcttttctc acagcagggg gtatcatcca cctcgctggg tgagattgca
     6961 aaagcagctg gcgttacgcg cggtgcaatc tactggcatt ttaaagacaa gtcggatttg
     7021 ttcagtgaga tctgggaact gtcagaatcc aatattggtg aactagagct tgagtatcag
     7081 gcaaaattcc ctggcgatcc actctcagta ttaagagaga tattaattca tgttcttgaa
     7141 tccacggtga cagaagaacg acgtcgatta ttgatggaga ttatattcca caaatgcgaa
     7201 tttgtcggag aaatggctgt tgtgcaacag gcacaacgta atctctgtct ggaaagttat
     7261 gaccgtatag aacaaacgtt aaaacattgt attgaagcga aaatgttgcc tgcggattta
     7321 atgacgcgtc gggcagcaat tattatgcgc ggctatattt ccggcctgat ggaaaactgg
     7381 ctctttgccc cgcaatcttt tgatcttaaa aaagaagccc gcgattacgt cgccatctta
     7441 ctggagatgt atctcctgtg ccccacgctt cgtaatcctg ccactaacga ataaccctga
     7501 atctgactcc aggatttttc ctggacattt tcgtcgttgc tattctggtt cactgcgtcg
     7561 tgatattctt gcggtttgac tttttcaggt cgttcttcag gttcagaaac cttcattcat
     7621 catgactatg ttccagtatt acaaacgatc acggcatttt gttttttcag catttattgc
     7681 ttttgttttt gtcttgttat gccagaacac ggcgtttgcg cgggcgtcat cgaatggtga
     7741 tctgccgaca aaagcggacc tgcaggcgca acttgactca ctaaataaac aaaaagatct
     7801 ttctgctcag gacaaactgg agctgcagga tctgacagat acattagcca ccctcgataa
     7861 aatcgatcgc ataaaagaag agacagttca gctacggcaa aaagtcgctg aagcgccgga
     7921 aaaaatgcgc caggcgaccg cggcgttaac agcacttagc gatgtcgata acgacgaaga
     7981 aacgcgcaaa attctgagta cgctttcgtt gcgccagctg gaaactcgcg ttgcccaggc
     8041 gctggacgat ttgcaaaacg cacaaaacga tctggcgtct tataacagcc agctggtttc
     8101 gttacagacg cagcccgaac gcgtgcaaaa tgcgatgtat aacgcttcgc agcagctgca
     8161 acaaattcgc agtcgtctgg atgggactga tgtcggcgag actgccttac gtcccagcca
     8221 gaaagtgtta atgcaggctc agcaggcgtt gctgaatgcg gagattgacc agcagcgtaa
     8281 aagcctggaa gggaacaccg tcttgcagga taccttgcaa aagcaacgtg attacgtgac
     8341 ggcgaacagc gcccgtctgg agcaccagtt gcagctgttg caagaagcgg taaacagcaa
     8401 gcgcctgact ttaactgaaa aaacggcgca ggaagccgtc tccccggatg aagccgcgcg
     8461 tattcaggct aatccgctgg tgaaacagga actggaaatt aaccagcagt taagtcagcg
     8521 tctgattacc gcgactgaaa acggtaatca gttgatgcag caaaacatta aagtcaaaaa
     8581 ctggctggag cgggcgctgc aatcggaacg caatattaaa gagcagattg ccgtcctgaa
     8641 gggcagcctg ctgttgtctc gtatccttta ccagcaacaa caaacgctgc cctcggcgga
     8701 tgaactggaa aacatgacca accgcatcgc ggatttgcgt ctcgaacagt ttgaagttaa
     8761 ccagcagcgt gatgcactct tccagagcga tgcgttcgtc aacaaactgg aagaaggtca
     8821 caccaacgaa gtcaacagcg aagttcacga tgcgttattg caagtggttg atatgcgtcg
     8881 cgaattgctg gatcaactca acaaacagtt gggtaaccag ctgatgatgg ccattaacct
     8941 gcaaatcaac cagcagcagt taatgagcgt atcgaaaaac ctgaaatcca tcctgactca
     9001 gcaaatcttt tgggtgaaca gtaaccgtcc aatggactgg gactggatca aagcgttccc
     9061 gcaaagcctg aaagatgaat ttaagtcgat gaaaatcacg gtgaactggg aaaaagcctg
     9121 gcctgccgtt tttatcgctt tcctcgccgg tttgccgctg ctgttgattg tcgggctgat
     9181 ccactggcgt ctgggctggc tgaaagcgta tcaacaaaaa ctggcttccg ctgtgggttc
     9241 cctgcgtaac gatagccagc tcaacacacc aaaagcgatc cttatcgacc tgatccgcgc
     9301 gctgccggtg tgcctgatta ttctcgcggt tggcttgatt ctgttgacca tgcagctcaa
     9361 catcagcgaa ctgctatggt cgttcagcaa aaaactggcg attttctggc tggtgtttgg
     9421 cctgtgctgg aaggtactgg agaagaacgg cgttgccgtg cgtcacttcg gcatgccgga
     9481 acagcagacc agccactggc gtcggcaaat tgtccgcatc agtctcgcat tgctgcctat
     9541 ccatttctgg tctgtggtgg cagaactttc cccgctgcat ctgatggatg atgtgctggg
     9601 gcaagcgatg attttcttca acctgctgct gattgccttc ctggtatggc caatgtgccg
     9661 cgaaagctgg cgtgataaag agtcgcacac catgcgactg gtcaccatta ccgtgctgtc
     9721 gattatcccg attgcattga tggtgctgac tgccacgggt tatttctaca ccacgctgcg
     9781 cctctccggg cgttggattg aaaccgttta tctggtgatc atctggaact tgctctacca
     9841 gacggtattg cgcggcttaa gcgtagcggc gcggcgtatc gcctggcgtc gtgcgctggc
     9901 gcgtcggcag aatctggtga aagagggcgc agaaggtgcc gaaccgccgg aagaacccac
     9961 cattgcactg gaacaggtta accagcagac gctgcgtatt accatgttgc tgatgtttgc
    10021 gctgttcggt gtcatgttct gggcaatttg gtccgatttg atcaccgtgt tcagctatat
    10081 cgacagcatt acgctctggc attacaacgg tactgaagct ggcgctgcgg tggtgaaaaa
    10141 cgtcaccatg ggcagtctgt tgtttgcgat tatcgcctca atggtggcct gggcgttgat
    10201 tcgcaacctg cctggtttgc tggaagtgct ggtgctctcg cgactgaata tgcgccaggg
    10261 cgcgtcgtat gccattacta ccatccttaa ctacatcatt attgccgttg gtgcgatgac
    10321 ggtgttcgga tcgctgggcg tctcttggga taaactccag tggctggcgg cagcattatc
    10381 cgtaggtctt ggttttggtt tacaagaaat tttcggtaac ttcgtctccg gtttgatcat
    10441 tctgttcgaa cgtccggtgc gtattggcga tacggtaacc attggtagct tctcggggac
    10501 ggtaagtaag atccgtattc gtgcgacaac gattaccgat ttcgatcgca aagaagtgat
    10561 catcccgaac aaagcgtttg ttaccgagcg tctgatcaac tggtcgttga ctgacactac
    10621 tacgcgtctg gtgatccgtc tcggcgtggc ctatggctcc gatctggaaa aagtgcgtaa
    10681 agtgttactg aaggcggcga ctgagcaccc aagggtgatg cacgaaccaa tgccggaagt
    10741 cttctttacg gcgtttggtg ccagcacgtt ggatcatgag ctgcgtctgt atgtgcgtga
    10801 actgcgtgac cgtagtcgta ctgtcgatga gctgaaccgt actatcgatc agctgtgccg
    10861 tgaaaacgac atcaacattg cctttaacca gcttgaagtg catctgcaca acgagaaggg
    10921 cgatgaggtg acggaagtaa aacgcgacta caaaggcgat gacccgacgc cagcggtagg
    10981 gtaataacca aagggggcga catttagttg ccccgagatt actgacaaag tgcgcgttgt
    11041 tcatgccgga tgcggcgtga ccgccttatc cggcctacga aaccgcaaga attcaatgta
    11101 ttgcaggagc ggtgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgcccac
    11161 aatcttaggg gacatttagc ggccccattt atttctcact tttccgcctc atcatcgcgc
    11221 gttaatttct tttcataatc gcgttttaca atatccagcg cgcgcagcac ggtgcgggct
    11281 gggatctgat tttcttccag cagcacaatc aaatcgacag ccagtttgac atcgtcaggg
    11341 gcattttcca gtgacatact ctctccattg ctagcgggtt aagcgcgcta gcctgttttc
    11401 gattttttcc agcgcatggc ggcagcgtgc caggcgcgct tcataggctt ccacttcacg
    11461 atgcagcgtt tgctgttcca cgagatcggt cacccgcgcc aggcgggctc tgcgttcggc
    11521 aaccatctca cgtagccgcc gctcaaaatc ctgatgctga atacgtttac gctgccagcg
    11581 ggcaattttc ggtggcgcac tgtcccactc gcgcaatgac caggcggagg cttcacgcgc
    11641 gatggcttcc agttgtgccg ccagatgttc cgccagccag gccacttgcg gcagctgttg
    11701 ctgctcaact gcatgacgaa gcgcagccag attatcgcca gcctcatcga gacaagcctg
    11761 cagtgttgtc gtacgggttt gaaaaaggtg tctgtcaaaa cgggcactta gcgtggcaaa
    11821 ttgcgctacc ggggcacaac gctgacgcag cgtagcgagc tgtccttcca gtttttccag
    11881 cagcagggcg gttttcacga taagacctca atgaaaatga taattgttat gctaaagtag
    11941 ccactctgta agctgacaac actcaatatg caacgaatca ttttaatcat cattggctgg
    12001 ctggcggtag tgctgggtac gctgggcgtg gtattaccgg tattaccgac gacgccgttt
    12061 atcctgctgg cggcctggtg ctttgcccgt tcttccccgc gctttcacgc ctggttgctg
    12121 taccgctcat ggtttggcag ctatctacgt ttctggcaga aacatcatgc gatgccgcgc
    12181 ggcgtcaaac cgcgggcgat tttgcttatt ttgctcacgt ttgccatttc tctgtggttc
    12241 gtccagatgc catgggtgcg catcatgttg ctggtaattc tcgcctgttt gcttttctat
    12301 atgtggcgaa ttccggtgat tgatgaaaag caagaaaagc actgaagcac aacaatcgca
    12361 gttgcaatta ttgcgtacag ccagtacatt ctggcgtttt cgagcacagg cgcaggcggt
    12421 caaaggttaa acaactgtta cttttgatac gtttaaaacg cgccgtgagt accaccgtaa
    12481 caagcaggca tacacttatg accgcgactg cacagcagct tgagtatctc aaaaatagca
    12541 tcaaaagcat tcaggactac ccaaaacccg gcattctttt ccgcgatgtc accagcttac
    12601 tggaagaccc gaaagcttac gctctcagca tcgacttgct ggttgagcgt tacaaaaatg
    12661 cgggcattac caaagttgtc ggcaccgaag cgcgtggctt cttgtttggc gctccggtag
    12721 ctctgggtct gggcgttggc tttgtaccgg tccgtaaacc gggcaaactg ccgcgtgaaa
    12781 ccatcagtga aacttacgac ctggaatacg gcaccgatca gctggagatc cacgttgatg
    12841 ccatcaaacc gggcgacaaa gttctggtgg tggacgatct gctggcaacc ggcggcacta
    12901 tcgaagcgac cgttaaactg atccgtcgtc tgggtggtga agtggctgac gctgcgttca
    12961 ttatcaacct gttcgatctc ggcggcgaac agcgtctcga aaaacagggc attaccagct
    13021 acagccttgt cccgttcccg ggccattaat tatcgccagt cttgtgctgc ccacgctacg
    13081 gacagcacaa gatgtgcatt cagcctcgcc gttctgacgg ggctgtgtta gcattacccc
    13141 ttcgtgaatc caccttccag cgtttcagag cctgccaatg agttatcagg tcttagcccg
    13201 aaaatggcgc ccacaaacct ttgctgacgt cgtcggccag gaacatgtgc tgaccgcact
    13261 ggcgaacggc ttgtcgttag ggcgtattca tcatgcttat cttttttccg gtacccgtgg
    13321 cgtcggaaaa acctctatcg cccgactgct ggcgaagggg ctaaactgcg aaaccggcat
    13381 taccgcgacg ccgtgcggcg tgtgcgataa ctgtcgtgaa atcgagcagg ggcgctttgt
    13441 cgatctgatt gaaatcgacg ccgcctcgcg caccaaagtt gaagataccc gcgacctgct
    13501 ggataacgtc cagtacgctc cggcgcgtgg tcgtttcaaa gtttatctga tcgacgaagt
    13561 gcatatgctg tcgcgccaca gctttaacgc actgttaaaa acccttgaag agccgccgga
    13621 gcacgttaag tttctgctgg cgacgaccga tccacagaaa ttgccggtga cgattttgtc
    13681 gcgctgtttg caatttcatc tcaaggcgct ggatgtcgag caaattcgcc atcaacttga
    13741 gcacatcctc aacgaagaac atatcgctca cgagccgcgg gcgttgcaat tgctggcgcg
    13801 cgccgctgaa ggcagcctgc gagatgcctt aagtctgacc gaccaggcga ttgccagcgg
    13861 tgacggccag gtttcaaccc aggcagtcag tgcgatgctg ggtacgcttg atgacgatca
    13921 ggcgctgtcg ctggttgaag cgatggtcga ggccaacggc gagcgcgtaa tggcgctgat
    13981 taatgaagcc gctgcccgtg gtatcgagtg ggaagcgttg ctggtggaaa tgctcggcct
    14041 gttgcatcgt attgcgatgg tacaactttc gcctgctgca cttggcaacg acatggccgc
    14101 cattgagctg cggatgcgtg aactggcgcg caccataccg ccgacggata ttcagcttta
    14161 ctatcagacg ctgttgattg gtcgcaaaga attaccgtat gcgccggacc gccgcatggg
    14221 cgttgagatg acgctgctgc gcgcgctggc gttccatcca cgtatgcctc tgcctgagcc
    14281 agaagtgccg cgccagtcct ttgcacccgt cgcgccaacg gcagtaatga cgccaaccca
    14341 ggtgccgccg caaccgcaat cagcgccgca gcaggcaccg actgtaccgc tcccggaaac
    14401 caccagccag gtgctggcgg cgcgccagca gttgcagcgc gtgcagggag caaccaaagc
    14461 aaaaaagagt gaaccggcag ccgctacccg cgcgcgtccg gtgccatcgg cgctggaaaa
    14521 agcgccagcc aaaaaagaag cgtatcgctg gaaggcgacc actccggtga tgcagcaaaa
    14581 agaagtggtc gccacgccga aggcgctgaa aaaagcgctg gaacatgaaa aaacgccgga
    14641 actggcggcg aagctagcgg cagaagccat tgagcgcgac ccgtgggcgg cacgggtgag
    14701 tcaactttcg ctaccaaaac tggtcgaaca ggtggcctta aatgcctgga aagaggagag
    14761 cgacaacgca gtatgtctgc atttgcgctc ctctcagcgg catttgaaca accgcggtgc
    14821 acagcaaaaa ctggctgaag cgttgagcac gttaaaaggt tcaacggttg aactgactat
    14881 cgttgaagat gataatcccg cggtgcgtac gccgctggaa tggcgtcagg cgatatacga
    14941 agaaaaactt gcgcaggcgc gcgagtccat tattgcggat aataatattc agaccctgcg
    15001 tcggttcttc gatgcggagc tggatgaaga aagtatccgc cccatttgat cgtaagcaca
    15061 gcttacgttc gtcatcctta acgtgattga gagagaaacc tatgtttggt aaaggcggtc
    15121 tgggtaacct gatgaagcaa gcccagcaga tgcaagaaaa aatgcagaaa atgcaggaag
    15181 agatcgcgca gctggaagtc accggcgaat ctggcgcagg tctggtaaaa gtgaccatca
    15241 acggtgcaca caactgccgt cgcgtagaga tcgacccgag cctgctggaa gacgacaaag
    15301 agatgctgga agacctggtg gctgcagcat tcaacgacgc agcacgtcgt attgaagaaa
    15361 cgcagaaaga aaaaatggcc tctgtatcct ccggaatgca gctgccgcct ggctttaaga
    15421 tgccgttctg atgcaaacca gcccgctgtt aacacagctt atggaagcac tgcgctgtct
    15481 gtcgggcgtt ggcccgaagt cggcgcagcg tatggcgttc acgctgcttc agcgcgatcg
    15541 tagcggcggg atgcgtctgg cgcaggcgct cacccgggcg atgtcggaaa tcggccactg
    15601 cgccgattgc cgcactttca ccgaacagga agtctgtaac atctgttcga atccgcgtcg
    15661 tcaggaaaac ggtcaaatct gtgtggtgga gagtccggcg gacatctacg ccattgagca
    15721 gacggggcag ttttctggtc gttattttgt gttgatgggg catctgtcac cgctggacgg
    15781 catcggtccg gatgatatcg ggcttgatcg tctggaacag cgtctggcag aggaaaaaat
    15841 cactgaagtg atcctcgcca ccaatcccac ggttgaaggt gaagctaccg ctaactacat
    15901 tgccgagctt tgcgcgcaat atgacgtgga agctagccga atcgctcatg gcgtaccggt
    15961 tggcggcgaa ctggaaatgg tcgatggcac cacgctgtca cactcccttg ccgggcgtca
    16021 taagattcgt ttttaagcaa acgagagcag gatcacctgc tctcgcttga aattattctc
    16081 ccttgtcccc atctctccct catcctgttt ttaaccttaa aatggcatta ttgaggtaga
    16141 cctacatgaa aggacaagaa actcgtggtt ttcagtcaga agtgaaacag cttctgcacc
    16201 tgatgatcca ttctctctat tccaataaag aaatcttcct gcgtgagctt atctctaacg
    16261 cctccgatgc ggcggacaag ctgcgtttcc gtgcgctatc taacccggac ctgtacgaag
    16321 gtgatggcga actgcgcgtt cgtgtctctt tcgataaaga caagcgcacg ctgaccatct
    16381 ccgataacgg cgtggggatg acccgcgacg aagtgattga ccatctgggg actatcgcta
    16441 aatccggtac caaatcattc ctcgaatccc tgggttctga ccaggcgaaa gacagccagc
    16501 tgatcggtca gtttggtgtt ggtttctact ctgcgtttat cgtggccgac aaagtgactg
    16561 tgcgtactcg cgcggcaggc gaaaaaccag aaaacggcgt cttctgggaa tcggctggcg
    16621 aaggtgaata taccgttgcc gacatcacca aagaagatcg tggtactgaa atcaccctgc
    16681 atctgcgtga aggcgaagac gagttcctcg atgactggcg cgtgcgttcc atcatcagca
    16741 aatactccga ccatatcgcg ctgccggtag agatcgaaaa acgcgaagag aaagacggcg
    16801 aaaccgttat ctcctgggag aaaatcaaca aagcgcaggc gctgtggact cgtaacaagt
    16861 cggaaatcac cgatgaagag tacaaagagt tctacaaaca catcgcccac gactttaatg
    16921 atccgctgac ctggagccac aaccgcgttg aaggtaagca ggagtacacc agcctgctgt
    16981 acatcccgtc ccaggctccg tgggatatgt ggaaccgtga tcataaacac ggcctgaaac
    17041 tgtacgtcca gcgtgtgttc atcatggacg acgcagaaca gttcatgccg aactatctgc
    17101 gcttcgtgcg tggtctgatt gactccagcg atctgccgct gaacgtttcc cgtgaaatcc
    17161 tccaggacag cacggtaacg cgtaacctgc gcaatgcgct gaccaagcgt gtgctgcaaa
    17221 tgctggaaaa actggcgaaa gacgacgcgg aaaaatacca gaccttctgg caacagtttg
    17281 gcctggtact gaaagaaggt ccggcggaag atttcgctaa ccaggaagcg atcgccaaac
    17341 tgctgcgttt tgcttctacc cataccgatt cttctgcgca gaccgtatct ctggaagact
    17401 acgtttcccg catgaaagaa gggcaggaga aaatctacta catcaccgca gacagctatg
    17461 cggcagcgaa gagcagcccg cacctggaac tgctgcgtaa gaaaggcatc gaagttctgc
    17521 tgctttccga ccgcatcgat gagtggatga tgaactatct gactgagttc gacggtaaac
    17581 cgttccagtc ggtgtctaaa gttgacgagt cgctggaaaa actggctgac gaagttgatg
    17641 agagcgcgaa agaagcggag aaagcactga ctccgttcat cgaccgtgtg aaagccttgc
    17701 tcggcgagcg cgtgaaagat gtccgtctga ctcaccgtct gaccgatacg ccagcgatcg
    17761 tttcgaccga cgcggacgaa atgagcactc agatggcgaa actgttcgct gcggcgggcc
    17821 agaaagtgcc agaagtgaaa tacatcttcg aactgaaccc ggatcacgta ctggtgaaac
    17881 gtgcggcaga tactgaagat gaagcgaagt tcagcgagtg ggtagaactg ctgctggatc
    17941 aggcgctgct ggcagaacgc ggcacgctgg aagatccgaa cctgtttatt cgtcgtatga
    18001 accagctgct ggtttcctga tgtcatgccg gatgaccttt gtgtcatccg gcatttttct
    18061 tttcatcatc tgcactttcc gcaaattatc tcgccattaa ccgtttcagc ctcaggtgcc
    18121 tttcttgagg caatcgcctg ttggtggtat cgtttatcgc tttttcaaaa aattcgacac
    18181 attttaaggg gattttcgca atgcgtatca ttctgcttgg cgctccgggc gcggggaaag
    18241 ggactcaggc tcagttcatc atggagaaat atggtattcc gcaaatctcc actggcgata
    18301 tgctgcgtgc tgcggtcaaa tctggctccg agctgggtaa acaagcaaaa gacattatgg
    18361 atgctggcaa actggtcacc gacgaactgg tgatcgcgct ggttaaagag cgcattgctc
    18421 aggaagactg ccgtaatggt ttcctgttgg acggcttccc gcgtaccatt ccgcaggcag
    18481 acgcgatgaa agaagcgggc atcaatgttg attacgttct ggaattcgac gtaccggacg
    18541 aactgattgt tgatcgtatc gtaggccgcc gcgttcatgc gccgtctggt cgtgtttatc
    18601 acgttaaatt caatccgccg aaagtagaag gcaaagacga cgttaccggt gaagaactga
    18661 ctacccgtaa agacgatcag gaagaaaccg tacgtaaacg tctggttgaa taccatcaga
    18721 tgactgcacc gctgatcggc tactactcca aagaagcgga agcgggtaac accaaatacg
    18781 cgaaagttga cggcaccaag ccggttgctg aagttcgcgc tgatctggaa aaaatcctcg
    18841 gctaatcaag ccgcagcaga tctgctttcg ggcaggtctg ctcatccacc tcaggcaata
    18901 cctctcacag caattagttc ttcttcctca cttttccgct acaattatca acaagttgaa
    18961 tcgataagag gcggtaatgc gtcagactaa aaccggtatc ctgctggcaa acctgggtac
    19021 gcccgatgcc cccacacctg aagcggtaaa acgctatctg aaacaatttt taagcgacag
    19081 acgcgtggtt gatacctcac ggttgttatg gtggccattg ctgcgcggcg ttattttgcc
    19141 gctgcgctcg ccgcgtgtgg cgaagctgta tgcctctgtc tggatggaag gtggctcgcc
    19201 gctgatggtt tacagccgcc agcaacagca ggcgctggca caacgtttac cggagacgcc
    19261 cgtagcgctg ggaatgagct acggctcgcc atcactggaa agcgccgtcg atgaactcct
    19321 ggcagagcat gtagatcata ttgtggtgct gccgctttat ccgcaattct cctgttctac
    19381 ggtcggtgcg gtatgggatg aactggcacg cattctggcg cgcaaacgta gcattccggg
    19441 gatatcgttt attcgtgatt acgccgataa ccacgattac attaatgcac tggcgaacag
    19501 cgtacgcgct tcttttgcca aacatggcga accggatctg ctgctgctct cttatcatgg
    19561 cattccccag cgttatgcag atgaaggcga tgattacccg caacgttgcc gcacaacgac
    19621 tcgcgaactg gcttccgcac tggggatggc accggaaaaa gtgatgatga cctttcagtc
    19681 gcgctttggc cgggaaccct ggctgatgcc ttataccgac gaaacgctga aaatgctcgg
    19741 agaaaaaggc gtaggtcata tacaggtgat gtgcccgggc tttgctgcgg attgtctgga
    19801 gacgctggaa gagattgccg agcaaaaccg tgaggtcttc ctcggtgccg gcgggaaaaa
    19861 atatgaatat attccagcgc ttaatgccac gccagaatat atcgaaatga tggctaatct
    19921 tgttgccgcg tatcgctaaa gctgagcggt aaagaactga gcgccgtcgc gaagagcctc
    19981 gtcggcggtt ttcatcattc gtgaataatg caaaaaggca tgcagcgtac ctgggtagag
    20041 tttgaactca cagggctgct gatgcgccgc taacgtctgg taaagcagac ggctgtcatc
    20101 cagcagcgga tcgaactccg ccccggcaat aaaacagggc ggaacttcgc gagtgagatc
    20161 attattaaac agacagtaat acggtgactc gcggtccgcg tcgttgctta aatacgcctc
    20221 ttcatacatc tgcaaatcct gttgcgttaa gccatcccag acaccgccca acagacgacg
    20281 agtcacggaa tcccgtaatc cgtaaagccc ataccacagc aaaacacccg caattttacc
    20341 gcaatcgatc tgtttatcac gcaaccacaa cgcactggcg agcgccagca tggcacctgc
    20401 ggaatcaccg gcaaagccaa tgcgggacat attgatttga taatcctccg cctgctggtg
    20461 gaaataacaa caagcagcca caatttcctc tatcgcttgc ggaaaacgcg cttcaggtga
    20521 aagggggtaa tcaataccaa tcaccgtaca ttggctgtag cttgccagca ggcgcatgat
    20581 gcgatcgtgg gtatcgagat tgccgagaat aaaaccgcct ccatgcaaat aaaatagcgt
    20641 cgccgggcta tctggctgcg gacaaaagag acgtgtttcc acctgcccat attttgttgg
    20701 aaccatgtaa gctctggttg ccatttctgg agcgcccgca ttccagaatc ggcgctcaag
    20761 cgtgtaatac tgtcgttgct cagcaatcgt tcccgttgcg ggccaggacg gtaaatccgg
    20821 ctgaagagta ttcacaacgg tcttcatttc agcagaaata aggtccagaa caggtagttt
    20881 gttttccggc ttcatacaaa aactcctttc aaattacgtc attgtaagga accactgcca
    20941 tgaaaatgcg atcccgcctg ctgatattga aactggctgc gtctcgcgcg ctcccgtcag
    21001 attgtgttaa cattcgccgc tcagttaacc acccgtaaaa acaaccatga aatttcccgg
    21061 taaacgtaaa tccaaacatt acttccctgt aaatgcacgc gatccgctgc ttcagcaatt
    21121 ccagccagaa aacgaaacca gcgccgcctg ggtcgtaggt attgaccaga cgctggtcga
    21181 tattgaagcg aaagtggatg atgaattcat tgagcgttat ggattaagcg ccgggcattc
    21241 actggtgatt gaggatgacg tagccgaagc gctttatcag gagctaaaac agaaaaacct
    21301 gattacccat cagtttgcgg gtggcaccat tggtaacacc atgcacaact actcggtgct
    21361 cgcggacgac cgttcggtgc tgctgggcgt catgtgcagc aatattgaaa ttggcagtta
    21421 tgcctatcgt tacctgtgta acacttccag ccgtaccgat cttaactatc tacaaggcgt
    21481 ggatggcccg attggtcgtt gctttacgct gattggcgag tccggggaac gtacctttgc
    21541 tatcagtcca ggccacatga accagctgcg ggctgaaagc attccggaag atgtgattgc
    21601 cggagcctcg gcactggttc tcacctctta tctggtgcgt tgcaaaccgg gtgaacccat
    21661 gccggaagca accatgaaag ccattgagta cgcgaagaaa tataacgtac cggtggtgct
    21721 gacgctgggc accaagtttg tcattgccga gaatccgcag tggtggcagc aattcctcaa
    21781 agatcacgtc tctatccttg cgatgaacga agatgaagcc gaagcgttga ccggagaaag
    21841 cgatccgttg ttggcatctg acaaggcgct agactgggta gatctggtgc tgtgcaccgc
    21901 cgggccaatc ggcttgtata tggcgggctt taccgaagac gaagcgaaac gtaaaaccca
    21961 gcatccgctg ctgccgggcg ctatagcgga attcaaccag tatgagttta gccgcgccat
    22021 gcgccacaag gattgccaga atccgctgcg tgtatattcg cacattgcgc cgtacatggg
    22081 cgggccggaa aaaatcatga acactaatgg agcgggggat ggcgcattgg cagcgttgct
    22141 gcatgacatt accgccaaca gctaccatcg tagcaacgta ccaaactcca gcaaacataa
    22201 attcacctgg ttaacttatt catcgttagc gcaggtgtgt aaatatgcta accgtgtgag
    22261 ctatcaggta ctgaaccagc attcacctcg tttaacgcgc ggcttgccgg agcgtgaaga
    22321 cagcctggaa gagtcttact gggatcgtta agttatcgtc ggttcgtagg ccagataagg
    22381 cgttcacgcc gcatctggca tttggctctc gatgcctgat gcgacgctgg cgcgtcttat
    22441 catgcctaca tatttttcat attttacatc cggcaaccac cgtttacccc gtcaccacct
    22501 cacccgccgg tggcgtttcc agcagttcca gcatggtacg ggcgatttca cgctcgccca
    22561 tcactacctg attcgcacca cgttcggtga tatacgccac ttcatcgtca taatgggcgc
    22621 gggcaataat ctcaatatcc ggatttttcg cgcgggcaga taccacaatc tcacccgctt
    22681 cataaccgtt gggaatcgtc aggatcagcc attttgcaca ttccagatgc gccagttgca
    22741 taatttcttc gttcgccgca ttgcccaata ctgcgcggac cccgcgctct cgcagctcat
    22801 caacacgggt tcgtgacgtc tcaatcacca ccagcggaat atcagaggcg agcaatttct
    22861 cccccagcag gctgcctaca cgaccgtaac ccaccagtag cgcatggttg caaatatcca
    22921 ctgggatctg cttctcttct tcgattgcct cttccagcgt ctgctcttcc agcgtttcgg
    22981 tcttcgccag atatttctcc agtagtgcga acagtaccgg gttgagcata atcgacagga
    23041 tcgcccctgc cagtaccagg ttttgtccgg cctgcggcag taaattcaat gccattccca
    23101 gtcccgccag gataaacgcg aactcaccaa tctgcgccag gctggcggcg atggttaatg
    23161 ccgtacgttg ggagtgacca aacagtcgca ccaggaaaaa tgcggctaac gacttaccaa
    23221 acagaataat cgccagcgtc gccagcactg ccagcggttg ctgaatcaga attaacggat
    23281 caaacaacat cccgacggag acaaaaaaca gcaccgcaaa cgcgtcgcgc aatggcagcg
    23341 tatcgtgggc ggcacggtga ctcagttcag actcgttcag taccatcccg gcaaagaacg
    23401 caccgagtgc aaaggagaca tcaaacagct ctaccgcacc aaaggcaatc cctaacgcca
    23461 gcgccagcac cgacagggta aacagctcgc gagaaccggt tgccgcgctg cgtgccataa
    23521 tccacggcac cagacggcga cctaccagca tcataatggc gataaatgcg atcactttgc
    23581 cgatggtgat ccccatatcg actgcaagag tggcaaagcc cacatcgccc tgttccatca
    23641 ttcctgcaac tgcgggcagc aacaccagcg tcagaaccat taccaggtct tccacaatca
    23701 accaaccgat ggcgatttgc ccacgctgac tgtcaattaa ttgccgttct tcaagtgcgc
    23761 gcagtaacac cacggtactg gcggtggaaa gacataaacc gaacacgata ccggtcatta
    23821 acgaccagcc cagcacggca gagagcgcca tacccagcag cgtcgccacg gctatctggg
    23881 cgatcgcacc gggaatggcg atggccttta ccgccatcaa atccttcagc gaaaagtgca
    23941 aaccgacgcc aaacatcaac agaatgacgc ccagttcagc cagttccggc gcaagcttgg
    24001 tatcggcaac aaagcccgga gtgaatggtc ctgccagcac acccgctaac agatatccca
    24061 ccagaggaga aatacgtagt ttattggcca gcatgccgag gataaaggcg agcacaaggc
    24121 cgccaacaat ggtggtgata agcggggtgg cgtgatgcat tccgtctcct tttcctggtg
    24181 gttattgtcc atttttggcc gggaaaacca aaattacagg taatagttta tgacaatttc
    24241 attgatgatg ttcatgaata attattgaat tttgcagaaa aatggaatta gctgcaaaaa
    24301 aagcacggat cggaaaacag aaggcggttt aacagaggaa aggcttatgg cgactgtaaa
    24361 acgatgcagc caaagtttgc ctttggctgc aatgaaatca gtctttatgc cggttatcag
    24421 gcaggaatat ggtcaacatc cccaatagtg gcaggaaagc acagatttta tagactaact
    24481 cgatgctggt gtgatcggcg ataagcccca gaactgccgc tcccagacct cccatgccaa
    24541 aagcaaaacc gaaaaagagt ccagaaacca taccgatacg tcccggaagt agctcctgag
    24601 cgtagaccag aatggcagag aatgccgaag cgaggataaa tccaataatc accgttaaaa
    24661 cccccgtcca gtgcaggctg gcgtagggta aaatcagcgt aaacggcgca acgccgagga
    24721 tagagcccca aatcacatat ttccgtccaa ttttatcccc tacaggcccg ccgatcaccg
    24781 tacctgccgc aacggcaaac aggaaggcaa acagatgaag ctgggcattc tggatagata
    24841 atccgaattt ttgcatcaga taaaaggtgt aatagct
//
LOCUS       DAJTOR010000045        24632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTOR010000045 DAJTOR010000000
VERSION     DAJTOR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.045"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..421
                     /gene="speG"
                     /locus_tag="OO249_001643"
     CDS             <1..421
                     /gene="speG"
                     /locus_tag="OO249_001643"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCR5635988.1"
                     /translation="TTSHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIIS
                     PEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHE
                     FFINGQYRNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(424..1134)
                     /locus_tag="OO249_001644"
     CDS             complement(424..1134)
                     /locus_tag="OO249_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5635989.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            1242..1547
                     /locus_tag="OO249_001645"
     CDS             1242..1547
                     /locus_tag="OO249_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5635990.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSANVSAPAQSTQDGAPAEPQ"
     gene            1746..2312
                     /locus_tag="OO249_001646"
                     /pseudo
     CDS             1746..2312
                     /locus_tag="OO249_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            2294..2422
                     /locus_tag="OO249_001647"
                     /pseudo
     CDS             2294..2422
                     /locus_tag="OO249_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
     gene            2465..3079
                     /gene="dmsD"
                     /locus_tag="OO249_001648"
     CDS             2465..3079
                     /gene="dmsD"
                     /locus_tag="OO249_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5635991.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQYEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELSRLTLAQWQSQLLIPVAVKPLFR"
     gene            3238..4530
                     /gene="clcB"
                     /locus_tag="OO249_001649"
     CDS             3238..4530
                     /gene="clcB"
                     /locus_tag="OO249_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5635992.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4483..5178)
                     /gene="bioD"
                     /locus_tag="OO249_001650"
     CDS             complement(4483..5178)
                     /gene="bioD"
                     /locus_tag="OO249_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5635993.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(5303..6523)
                     /gene="mlc"
                     /locus_tag="OO249_001651"
                     /gene_synonym="dgsA"
     CDS             complement(5303..6523)
                     /gene="mlc"
                     /locus_tag="OO249_001651"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5635994.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNLGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPIISDSIRQQALPTYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLHG"
     gene            complement(6658..7551)
                     /locus_tag="OO249_001652"
     CDS             complement(6658..7551)
                     /locus_tag="OO249_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5635995.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRAQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            7658..8910
                     /locus_tag="OO249_001653"
                     /pseudo
     CDS             7658..8910
                     /locus_tag="OO249_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9307..9621
                     /locus_tag="OO249_001654"
     CDS             9307..9621
                     /locus_tag="OO249_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5635996.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTTKAAPAKT
                     THHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQP
                     AA"
     gene            9714..9797
                     /locus_tag="OO249_001655"
     CDS             9714..9797
                     /locus_tag="OO249_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5635997.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            9897..10718
                     /locus_tag="OO249_001656"
     CDS             9897..10718
                     /locus_tag="OO249_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5635998.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVVLRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(10757..11086)
                     /gene="mdtI"
                     /locus_tag="OO249_001657"
     CDS             complement(10757..11086)
                     /gene="mdtI"
                     /locus_tag="OO249_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5635999.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(11073..11438)
                     /gene="mdtJ"
                     /locus_tag="OO249_001658"
     CDS             complement(11073..11438)
                     /gene="mdtJ"
                     /locus_tag="OO249_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5636000.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            11850..12884
                     /gene="tqsA"
                     /locus_tag="OO249_001659"
     CDS             11850..12884
                     /gene="tqsA"
                     /locus_tag="OO249_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5636001.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(12909..14297)
                     /gene="pntB"
                     /locus_tag="OO249_001660"
     CDS             complement(12909..14297)
                     /gene="pntB"
                     /locus_tag="OO249_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5636002.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQDVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(14308..15840)
                     /gene="pntA"
                     /locus_tag="OO249_001661"
     CDS             complement(14308..15840)
                     /gene="pntA"
                     /locus_tag="OO249_001661"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5636003.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            16364..17308
                     /locus_tag="OO249_001662"
     CDS             16364..17308
                     /locus_tag="OO249_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5636004.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            17494..18876
                     /locus_tag="OO249_001663"
     CDS             17494..18876
                     /locus_tag="OO249_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5636005.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            18913..19635
                     /gene="folM"
                     /locus_tag="OO249_001664"
     CDS             18913..19635
                     /gene="folM"
                     /locus_tag="OO249_001664"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5636006.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKLGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(19632..19944)
                     /locus_tag="OO249_001665"
                     /pseudo
     CDS             complement(19632..19944)
                     /locus_tag="OO249_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
     gene            20070..20789
                     /gene="rstA"
                     /locus_tag="OO249_001666"
     CDS             20070..20789
                     /gene="rstA"
                     /locus_tag="OO249_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5636007.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGITICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     MPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRNVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            20793..22094
                     /gene="rstB"
                     /locus_tag="OO249_001667"
     CDS             20793..22094
                     /gene="rstB"
                     /locus_tag="OO249_001667"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5636008.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            22170..23099
                     /gene="tus"
                     /locus_tag="OO249_001668"
     CDS             22170..23099
                     /gene="tus"
                     /locus_tag="OO249_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5636009.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHCDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(23096..24499)
                     /gene="fumC"
                     /locus_tag="OO249_001669"
     CDS             complement(23096..24499)
                     /gene="fumC"
                     /locus_tag="OO249_001669"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5636010.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         5947 a         6342 c         6020 g         6323 t
ORIGIN      
        1 gaccacttca catattcacg atcagagcga acgacgcttt gtggtggaat gtgacggcga
       61 aaaagccggt ctggtggagc tggtggaaat taaccatgta catcgccggg cagaatttca
      121 gataattatc tccccggagt atcaggggaa aggtctggca acccgtgccg ccaaattagc
      181 aatggactat ggcttcaccg ttctcaatct ctataagctg tatctgatcg tcgataaaga
      241 gaatgaaaaa gcgattcaca tttaccgcaa gcttggcttt acggttgaag gtgaattgat
      301 gcatgagttt tttattaatg gtcaatatcg taatgccatt cgcatgtgta tattccagca
      361 tcagtatctg gcagagcaca aaacaccggg tcagactctc ctgaagccga ccgcacaata
      421 gcattaataa taatcgatcg tatttttgat ggtgtaaacc cgttcgacgg cgggttttac
      481 tccttcatca acaatgatca gctgacagtc caccggattg gcgtgactgt catattcaca
      541 gctctgtttt acattaccaa ctcgttgatt attcagtaaa gtaaccgctg tgtaatctaa
      601 ttttttgatc gggtccgttg atggcgtggc gctgacagat aatgttttgt cgttactttt
      661 cgtggtttta cccagtggat aaccctgatc atcatagcga tattccatct gcatttgttt
      721 gctgctggct ttaatcacga agccattatc atcggtttcc caactcaccc cggcagaagg
      781 taattctgct agctggcatt ttccctgtaa acgtactctc ttctccagcg tctcggcatc
      841 acggtaataa ttggcgtcca gtaccagagc gaccacggta ttattttcca gatccagtaa
      901 ttcgagtgaa tcaaaacagc cttcttccga caaagtccca gaaacacgtt tcgtcacttc
      961 accttgctca tccattaatg tctgagtgaa atcttttacc ggaccacgca gcggatcaaa
     1021 atcgaattca ttagagaaac tggccatctc aggggtaaat gaaagtgtta cctctgtgcg
     1081 gtcacatcct gtgaggaata tcgcgagtaa gcatggtagt aatttgtatt tcacaacagt
     1141 caccagagag tagagatgat tctcaatcat agtagcaaat acagtacttt acacgttaaa
     1201 tgctatgctt aaagaagtta tcttcgcgta aggagcttat gatgaaactc tcaacctgct
     1261 gtgccgcgct tctgctcgcc ctcgcctcac ccgcggtact cgccgcgccg ggatcctgtg
     1321 agcgcataca gagcgatata tcacagcgca ttatcaataa tggcgtaccc gaaagcagct
     1381 tcacattaag tattgtaccc aatgaccagg ttgatcagcc tgattcccag gtcgtcgggc
     1441 attgtgctaa tgatacgcat aaaattctct acacccgcac caccagcgct aacgtctctg
     1501 ctcccgcgca gtctacccag gatggcgcgc ctgccgaacc gcagtaatac attatcgact
     1561 gaacgccgga tatgacacat ccggcgattt gaacatacaa cataatccca ccttattact
     1621 catacccttc tattgatgtg gattaataat tcttaaccca aaatgggtag accccctcta
     1681 ttgttagcgc gctaaatatt caatacataa acatttatat aacgataaag aacagggagt
     1741 gagttatgtc caaaaatgac cgaatggtgg gcatcagccg cagaacactc gttaaatcaa
     1801 ccgcgatagg ttctctggcg ctggctgcag gcggtttttc tttaccgttc accctgcgca
     1861 gtgcagcggc aacggtacaa caggccagcg aaaaagtgat ctggggtgcc tgttccgtca
     1921 actgtggtag ccgctgtgca cttcgtctac atgttaaaga taatgaagtg acctgggtgg
     1981 aaactgacaa taccggcagc gatgagtacg gcaaccatca ggtacgcgcc tgtttgcgcg
     2041 gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaattaccca atgaaacgcg
     2101 tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc ctggatacta
     2161 tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaggctgta tatattcagt
     2221 actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc tcggcggtca
     2281 aacgcctgat gaactgctac ggcggttccc tcacgttgcc ggacttattc tggggctaat
     2341 cctcatactt ggcggtgaga tgatcgggcg tgtgctcttt tatggcctgc acatgacagt
     2401 aggaatggct attgcaggtt aacaaattgg tgcggggcaa cccgcacatt tcaggatgtt
     2461 aggaatgacc catttttcac agcaagataa tttttctgtc gcggcgcgcg tgttgggcgc
     2521 gctgttttat tacgctccag agagtgcgga agccgcacct ctggttgcgg tactcaccag
     2581 tgacggctgg gaaactcagt ggcctctacc agaagcgtca ttagcgccgc tggtgacggc
     2641 ttttcagaca cagtgtgaag agactcacgc ccaggcctgg cagcgtttgt ttgtcggccc
     2701 gtgggcactg ccgtctccgc cgtgggggtc ggtctggctg gatcgcgaat ctgtgctgtt
     2761 tggcgattca acattggcac ttcgtcagtg gatgcgcgag aaaggcattc agtttgaaat
     2821 gaagcaatac gaaccggaag atcattttgg ttcactgctg ttgatggctg cgtggttggc
     2881 ggagaatggt cgccagacag aatgcgaaga attgctggca tggcaccttt ttccgtggtc
     2941 aacacgtttt cttgatgttt ttatcgaaaa agcagaacac cctttctacc gtgcactggg
     3001 tgaactgtct cgcttaacgc tggcgcaatg gcaatcacaa ctgttaattc ctgtcgcggt
     3061 taaaccgctg tttcgatagg atcaccgtaa tattgccgga tggtgacgat tacgcgcaat
     3121 ccggcaataa taggttacag tgtcacgttt ttttatctct taaagcacgc actgcttttg
     3181 cggctggcct cttttgccgc aaaatagtcg cccgtgtttc attgcccatt tctgctcatg
     3241 catcatctac acatctatcc ggatctgcgc actatgttcc gccgtctgct tatcgcaaca
     3301 gtcgtcggta ttctcgcggc ctttgccgtt gccgggtttc gtcatgcgat gctgctactg
     3361 gagtggttgt tcctcaataa tgactccggc agtctggtca atgcagcgac aaacctttcc
     3421 ccctggcgac ggttgctaac tccggcgctc ggcggactgg cggcgggttt gttgctgatg
     3481 ggctggcaga aatttaccca acaacgccct catgcgccga ccgattacat ggaagcgttg
     3541 caaaccgatg gacagttcga ttacgcagca agcctggtta aatcgcttgc ctcgctgctg
     3601 gtagtaacca gcggcagtgc aattggtcgc gaaggtgcga tgattctttt agctgccctt
     3661 gccgcctcct gttttgccca acgttttacg ccacgccagg agtggaaatt atggatcgcc
     3721 tgtggcgccg cggcgggaat ggctgcggcc tatcgtgccc cgcttgctgg cagtttattt
     3781 atagccgaag tgctgtttgg cactatgatg ttggcctctc tcggcccggt gattatttcc
     3841 gccgtcgtgg cattgctggt tagcaatctg attaatcata gcgacgcgtt actctacaac
     3901 gtacaactct cagtgacggt tcaggctcgt gactatgcgc tgattatcag tacaggtgtg
     3961 ctggcaggtc tgtgcggacc actgttgtta acgttaatga acgcctgtca tcgtggattt
     4021 gtgagtctca aacttgcgcc gccctggcaa ctggcactag gcgggttgat cgtgggtctg
     4081 ctttccctgt tcacacctgc agtgtggggc aacggctata gcaccgtaca atccttttta
     4141 accgccccac cactgttaat gatcattgcc gggatcttcc tttgtaaact gtgtgccgtg
     4201 ctggcgagta gcggttccgg cgcacccggt ggtgtcttta caccgacgct atttatcggt
     4261 cttgccattg gcatgttgta tggtcgtagc ctgggattat ggttccctga tggcgaagaa
     4321 attacgcttt tactcggatt gaccgagatg gcgacactgt tggcggcaac cacgcacgcg
     4381 ccgattatgt cgacgttgat gatatgtgaa atgaccgggg agtatcagct actccccggt
     4441 ttattgattg cctgcgtcat tgcgtcggta atttcgcgga cgttacaccg tgactctatc
     4501 taccgccagc acactgcgca gcatagctaa gcggatgtat tgccccagtt ctcgctgttc
     4561 ggcgcgcggc agataaggca gttcaccaat gagcggtgcc ggaagttttt tacccagcac
     4621 atcaatgatt tccgcataat gcgccagtcc tgggttgatt cggttagcca cccagccaat
     4681 gagcggcagc ccgtcgttgg caatcgcctg agctgttagc agtgcatggt taatgcaacc
     4741 ttcctgaata ccgacaacca tcaacaccgg caactgttcc tgcaccaccc attcagagag
     4801 tggacgcaaa tcattcatca gactgcgcca gccgccagtc ccttctacca cgacatgatc
     4861 gactttatcg gtcaggtttg ccaggccgtt tgaaatgagg gtgtaattga ttgggcaact
     4921 gtgcgccacg ctactttctt cttcgcttaa cgcgatagga ttaactgctt cataaggtag
     4981 ttcgatggtt gaaacactct gcaacaccag ggcatcttta ttacgcagcc cttcgggtgt
     5041 ctctttgctc cccttcgcta cgggtttata tcccgcaact gtttttccct gggaggctaa
     5101 cgcttgtagc aatgcgcggg aaaccaccgt tttccctaca gaagtgtctg taccggtaat
     5161 aaagaaacgc ttcagcatca ctaactccac cgttatgctt cacaaatata aactaggaaa
     5221 ataattaacc ttgaaagtct aagttatgct ttcctggccc aaattgagat agcgcaaatt
     5281 ttggtagaac agttaaaaaa tgttaaccat gcaacagacg aatcaacaaa gaaccgttat
     5341 acatcgcgtc ttttaccagc gcagcgcctg ccatcgtgcc ctggttagaa aactgagtac
     5401 tctcaacgct aatgtgctga ctatacgtag gaagggcctg ctgacgtatg ctgtccgaga
     5461 tgatagggaa gaggatatct gccgctttac ttaacggtga gccaatcagt attttttgtg
     5521 ggttaaataa attcaccatg atggcaagaa tgcgcccgac atgcgcgccc accccggtaa
     5581 tgatgtcttt tgccagtaga tcgccgcgca atgccgcctg acacaatgag tccacggtta
     5641 acggttgccc atgtaacatc gagctcatgg attgattgag acgcagctgt gccagctcaa
     5701 gaatactgtc cacactggcg atggtttcga ggcagccgag gttcccgcaa taacagcgtt
     5761 taccatatgg gtcgacctgt gtgtggccta tttccacgag actactgctg cctgcgtgta
     5821 gcagatgacc atcggtaatg acgcccgccc ccacgttgtg atcgataacc acctgaatca
     5881 catcgcgcgc cccgcgtgag gcaccaaaca aggcctctgc catcgtccat gcgctgatat
     5941 catgctgaat ataaaccggt acgccggtat gctgctccag cgcctcgccg agcggcatct
     6001 cttttacatc ctcgtagaac ggcatgcgat gtacaatacc attttccgta tcaataattc
     6061 ccggcagggt tatggcaatc gaagttagac gctcaagttt tttctggtgg cggataaaaa
     6121 actgatcgat atgggaaatg atacgatcca gcaatggcaa gtcatctttt aacgccagtt
     6181 cctgcgactc ttccaccacc agtttgctgc tcagatcgcg cagagcaagg aaaatctcac
     6241 cgcgactaat gcgcagagaa agatagtgcc aggcttcagt ttcaaccacc agtcccaccg
     6301 ccggacggcc acggttcccc gcttctttga tttccagctc ttgcaccaag tgtgcttcga
     6361 gcatctcacg gacaatttta gtgatactgg caggagccag ctgcgccaga cgggaaagat
     6421 cgatacgcga gactggacca agctgatcaa tcaggcgata aaccgcgccc gcgttggtct
     6481 gctttatttg atcaatgtgc ccaggctggt tttcagcaac caccgcatac tccctatatt
     6541 ttcgcgctcc gaaataatct gtaggctatg gtgaagcact tcaatacgtg tcgtcaaatt
     6601 tttacttaga catgtgatta acagcacatt tttcgggctt tttcgctgaa atttccctca
     6661 cctgagagca ttcagcagat gaatacgaaa gttacgcgaa gctggacttt gttcatgatg
     6721 tttcggccag accaaccaca tttcagaaac cgcatcctct tcagcaatcg gcacccagcg
     6781 catttcgttg agctgagcac gtttaaatga agcaggcaaa attgaaacac ccagaccggc
     6841 ggaaaccaga ccgatgatgg tcattgcctc gcccacctcc tgagtgatga cgggcgtcaa
     6901 atggtaacgt cgcatcagcc cgagaatatc gtcatacagc cctgtcccga cgtgcggatc
     6961 aaaaaagacg aagggttctt tcgccagttc agccaacgtt acattcgggt tatttgccag
     7021 gggatgatcg tgcgggatca tcgccataag cggttcatgg acgatgactg cgtgttcaag
     7081 cgtctccggt aacgctgtgt tacgcagcaa tcccatatcc agcgttcctt caatgagcgg
     7141 agcgatttgc tcacgagtgt tcatttcgcg ggtttgtaaa tggacaccgg gataatcacg
     7201 gcgaaacagc gataacgtat cggacaccgc ccgaataaaa ggagcggacg aagtaaaacc
     7261 aatgcgcaac tcccccgctt caccctgatg cagtctttca gcgcgagcgg cagcgtcatc
     7321 taccatagac aggatttgcc gactatctgc aagaaactgt tttcctgctg ccgtcagcaa
     7381 tacactgcga ttagttcgtg ccagcagtcg ggcaccaatt tgttgctcca gtgcctgaat
     7441 ctgctgactt agcggtggtt gcgaaatatt caggcgggca gcggcgcgcc cgaaatgcag
     7501 ctcttccgca acagcaacaa agtaacgcag atgacgaagt tcaatattca tatttaaaac
     7561 atcttatttg agattattaa tatattagac agaacaattc gattttccta acctatgtat
     7621 aagcctgatc cacaggcata tttagcaagg atttcaagtg agccgtacta caactgttga
     7681 tggcgctccg gcaagcgaca ctgacaagca aagcatttct cagccaaatc aatttattaa
     7741 acgcggtacg ccgcaattta tgcgcgtcac cctggcgctg ttctctgccg gactggcaac
     7801 ctttgcactc ctctattgtg tgcagcctat ccttccggtg ctttcgcagg agtttggctt
     7861 aacccccgcg aacagtagta tttcactgtc catttccacg gcgatgttgg ctattggttt
     7921 gctgtttact ggccccctat ccgatgccat tggtcgcaaa ccagtgatgg tcacggcgct
     7981 actgttggcc tcaatttgta cgttatttcg acaatgatga ccagctggca cggcattttg
     8041 attatgcgcg ccttgattgg gctttcgtta agtggcgtgg cagctgttgg catgacttat
     8101 cttagcgagg aaatccaccc cagtttcgtg gccttttcga tggggttgta tatcagcggc
     8161 aactcaattg gcggcatgag cggacgctta attagtggtg tcttcacgga ctttttcaac
     8221 tggcgaattg ctctggcggc aatcggttgt ttcgcgctag cctcggcgtt gatgttctgg
     8281 aaaatccttc cggaatcacg ccattttcgc ccgacttcgc tgcgacctaa gacgttgttt
     8341 atcaactttc gtctgcactg gcgtgaccgg ggattaccgt tattgttcgc agaaggcttt
     8401 ttgctgatgg ggtcgttcgt cacgctgttt aattacatcg gctatcggtt gatgctttcc
     8461 ccctggcatg tcagtcaggc cgtggttggc ttattatcgc tggcttattt gaccggtaca
     8521 cggagctcac ccaaagccgg aaccatgacc acccgctacg ggcgcggtcc ggtgatgttg
     8581 ttttcgacgg gggttatgct gtttggttta ctgatgacct tattcagctc gctgtggctg
     8641 atctttgccg gaatgttact cttctcagca ggattcttcg cagcccactc agttgccagc
     8701 agctggatcg gcccccgcgc aaaacgcgct aaaggccagg cctcctcgct gtatctgttc
     8761 agttactatc tgggatcgag tattgccggg acgctgggtg atgttttctg gcataactat
     8821 ggctggaacg gcgtcggcgc atttattgct ctgatgctgg tcattgctct gctggtcggg
     8881 acgcgtttgc atcgtcgtct gcacgcctga aaaataagcc cggactgcgg taactacacg
     8941 tccggactta ttgcttgctc aaaccaacgt taatagccat cctaaaatag acgaagcgcc
     9001 agccaattcc cgcagtgcgt ctagcgtcat caggattata agtacccaaa taaacggatt
     9061 cattttgctg tgtgtcattt attactgatg cgcagttatt ctaccgcttt gtaagtagta
     9121 aaatagttaa cccgatcaag actactatta ttggtagcta aatttccctt aagtcacaat
     9181 acgttattat caatgctgta atttattcag cgtttgtaca tatcgttaca cgctgaaacc
     9241 aaccactcac ggaagtctgc cattcccagg gatatagtta tttcaacggc cccgcagtgg
     9301 ggttaaatga aaaaacaaat tgagggtatg acaatgaaaa aagtattagc tctggttgtt
     9361 gccgctgcta tgggtctgtc ttctgccgcc tttgctgcag agactgcgac gaccaccaaa
     9421 gcagcgccgg cgaaaacgac acatcataaa aaacagcata aagcagcacc tgcccagaaa
     9481 gcgcaggcag ctaaaaagca tcataaaaat acgaaagctg aacagaaagc ccctgaacaa
     9541 aaagcgcagg cagcgaagaa acacgccggg aaacacagtc atcagcaacc ggcaaaacct
     9601 gctgcacaac ccgcagcgta agttttcaac agtaatgctg gcgcgccccc tcgcgcctga
     9661 aaattacggt gctaagcggg taacgtttag caccgccttt agcggagggt agtatggtgg
     9721 gccgttatcg ctttgagttc attcttatca tccttatttt atgcgcactg attgccgccc
     9781 gtttttatct ttcctgactg tagttatctg attttactcc cactttcatc ccgtcccgtc
     9841 tatagtattt atgagggttt gcttttaata atcataatta cccaccagag tgtgatatgc
     9901 gtacaaccat tgctgtagtg ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag
     9961 ataaaccaga cgttgccaaa tcggcaaacg atgaggtcag caccctgttt tttggtcatg
    10021 atgatcgtgt gccagtgaat gacacggccc aatcaccgtg ggatgcggtt gggcaactgg
    10081 aaacggccag cggcaattta tgtacggcga cgctgattgc acccaatctg gcattaacgg
    10141 caggacactg tttattgaca cctccaaagg gtaaagccga taaagcagtg gtgctgcgtt
    10201 ttgtgtcaaa taaaggtctt tggcgctatg agatccacga catagaaggc cgcgttgatc
    10261 cgacactggg aaagcggtta aaagcagatg gggatggttg gattgtacct cccgcagccg
    10321 cgccgtggga cttcggattg attgtgctac gtaatccccc ttctggcatt acaccgttgc
    10381 cgttatttga gggagataaa gccgcgctta ctgccgcatt aaaatcggct ggccgtaaag
    10441 taactcaggc gggataccct gaagatcatc tcgatacgtt gtacagtcat caaaactgtg
    10501 aagtgactgg ctgggcgcaa acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg
    10561 acagcggttc gcctctgatg ttgcatacca atgacggctg gcaattaatt ggggtgcaaa
    10621 gttcggctcc tgccgcgaaa gatcgctggc gcgccgataa ccgggccatt tctgttaccg
    10681 gttttcgcga caagctggat caactgtcgc aaaaataatg ttcaaacgct gcccgactgt
    10741 gcgggcagcg tgttcatcag gcaagtttca ccatgatcat tccagccaac agcaagacca
    10801 ggccaatcca gcctttacga tttaaccgtt gaccaaacaa gatccaacct gcggctaacg
    10861 tggcggcaat accaaacccg ccccacaatg cataagcgac agacaagtcg atccctttaa
    10921 cggcctgaga aagcgcacta aaggcagcca gcaccgccgc cagggagagc aagccaaata
    10981 ttttgcgacg aaagccgtca gaaaatttca aaaagacgtt agcaacgatt tccagcacga
    11041 ttgccaatgc cagccaggcg gcgtgaaccc attcaaactg cgccatggtt tacctccagt
    11101 tcaggtttac gcgctttacg ggtacctgat tttatcaaca caatcccggc gaccagggtg
    11161 gttaacccgg caattttcat cagcgataaa ctttcgtcga ataacaaaac gctaaacaag
    11221 gtaataaata aaataccgat accttcccac aacgcataag ctacgcctaa ggcgatcttt
    11281 ttaacggcga aagagagaaa tatatacgac agagaaatca tcaccagcat taaaataaag
    11341 ccgccatttc cctcactgac gctcgcccat ttcattgaca gcgtaccggt aatttctgta
    11401 gcaatagcca ggcctaataa aatccaataa atatacattg tccttctcct gcaagagaat
    11461 tattttaatt ttcgcttaat tcagcgaaat caaagtaaag tggtcaagcc ccgcgcatag
    11521 ggcaagctta ggcagaagaa aggactaaag cgcgttgcgc cagtgctgct cacctacgag
    11581 caagatagtg gatgaggtac aaagatgggt aaatgttgaa aataaggtcc tgaacaaatt
    11641 gtccataata ttacaattat ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc
    11701 tcggtagttg aacacgcctg atttgtatca tagcttaaga attaactcaa aatattttca
    11761 cttctttacc tgagcggttt gattttcgtt atgatgacgg agcgaaaaag acattattat
    11821 tagcaaagga agaaaaaacg gggacaagca tggcaaagcc gatcatcacg ctcaatggcc
    11881 taaaaatcgt cattatgttg ggaatgctgg tcattattct ctgcggtatc cgttttgccg
    11941 ccgagatcat cgtgccgttt attctcgcat tatttattgc tgttattctt aacccgctgg
    12001 tgcaacacat ggtccgctgg cgagtgccgc gtgtactggc ggtgtcgatt ttgatgacca
    12061 tcatcgtgat ggcgatggtg ttgctattag cttatctggg ttccgcgctc aacgagttga
    12121 cgcggacgtt accgcaatat cgcaactcta ttatgacgcc gctgcaagct cttgagccgt
    12181 tgttgcaacg cgtagggatt gacgtctcag ttgaccagct ggcgcattac attgatccga
    12241 acgcagcgat gacgttgctc accaacttat tgacgcagtt atctaatgcc atgtcatcaa
    12301 tatttttatt gctgctgacg gtgctgttta tgctgctcga agtgccacaa ttgcccggaa
    12361 aatttcagca aatgatggcg cgtccggttg aagggatggc ggcgattcaa cgtgcaattg
    12421 acagtgtttc tcattatctg gtgctgaaaa cagccatcag catcatcacc ggcctggtcg
    12481 cctgggcgat gctcgccgca ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct
    12541 ttgcgcttaa ttacatccct aatattggtt cagttctcgc ggcaatcccc cctatcgctc
    12601 aggtactggt gtttaatggc ttctacgaag cgttgctggt gctggcggga tatctgctga
    12661 ttaatctggt cttcggcaat attctggagc cgcgcatcat gggacgtggg ctggggcttt
    12721 ccacattggt ggtatttttg tcgttgattt tttggggatg gttgttagga ccggtgggta
    12781 tgctgctttc cgtgccgtta acaattattg tcaaaattgc gcttgaacaa acagcgggag
    12841 gtcaaagcat cgccgttctg ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc
    12901 cgtcagggtt acagagcttt caggattgca tccacgctgg ctttggcgtc accaaacagc
    12961 atgtgggtgt tttccttgaa gaacagcggg ttttgcacac cagcatagcc agtgttcatc
    13021 gaacgtttaa agacaatcac gtcctgcgct ttccacactt ccagcacagg cataccagca
    13081 ataggactct tcggatcatc ctgcgccgcc gggttaaccg tatcgttagc accaatcacc
    13141 agtacggtat cggtatcagc aaagtcatcg ttgatctcgt ccatttccag cacgatgtca
    13201 tacggtactt ttgcttcagc cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc
    13261 gggtggatac cgaaacgcac gttgatacca cgagcgcgca gtttctcggt aatttcagcg
    13321 acaggatatt gcgcctgcgc gactgccatg ccgtaccccg gagtaatgat cactgaatgg
    13381 gagtttttca gcagttccgc tgtctcttct gcggtgattt cgcggtgctc gcccacttcc
    13441 tgatcatcgc cagtagaaga gccgtcggtg ccgaaaccac ccgcaataac gctgataaag
    13501 gaacggttca tcgccttaca cataatgtaa gaaaggatag cccccgaaga accgaccagc
    13561 gcaccggtca caatcagcag gtcgttgctg agcataaagc ccgcagccgc agccgcccag
    13621 ccggagtacg agttcagcat cgacaccacc actggcatat ctgcaccacc gatggaggcg
    13681 actaaatgcc agccgaatac cagcgcaatg gcggtcatta ttagcaatgc cagcacttgc
    13741 aggccgacgc tgtccgtgcg aacaaataca atcagcagca ggaaggaaac gaccagagcc
    13801 gccaggttca ttttgtgacg gtttggcagc atcaacggtt tagacgaaat cttgccacac
    13861 agtttgccga acgccaccac cgaacccgtg aacgttaccg ccccgatgaa gataccgagg
    13921 aacacttccg tcaggtgaat attgaccaga atcggtgcca ttcccgcgtc atgatgcaga
    13981 tagctgttaa agccaaccag cactgccgcc agacccacga agctgtgcag gatcgccacc
    14041 agttctggca tttcggtcat ttcaactttc ttcgccagac ggataccaat tgccccacca
    14101 atgaccatcg ccagcaagat ccagccaaca ttacccgtat ccggtccaaa aatggttgcg
    14161 attaacgcaa tcgccatccc tgcgataccg aagttgttac cctggcgaga cgtttcatgt
    14221 ttcgaaagac cggccagact gaagataaac aggatcgcgg caacaatgta tgcagctgta
    14281 actaatcctc cagacatatg ttacccctta atttttgcgg aacattttca gcatgcgctg
    14341 agtcacggtg aagccaccga aaatattaat gctggctata agcaccgcga taaaactaag
    14401 gaagctaacc cagccgccct ggccaatctg caacagtgct ccgacaacaa taatccctga
    14461 aatcgcgttg gtgaccgaca tcaacggtgt atgcagcgcg tgcgatacat tccacaccac
    14521 gtaataaccg acaacgcagg ccagcgcgaa aacggtgaag tgcccaagaa actctttcgg
    14581 cgcaacgctt gccagccaac cgaaaaggat gattgccagc gccatcaacg cgtatttacg
    14641 ccacggtgag caggcacatt tttcctcagt tttcacttcc ggtgccgctt tttgtgccgc
    14701 ctgcggctga gctgatacct gaatcggcgg tgccggccag gtaatttcgc ccgcacggat
    14761 cacggtcacg cggcgaatca ccacatcatc aaaatcaaca gtgatattgc cgtctttctc
    14821 tttgcacaac agtttcagca gattaacgag gttagtaccg taaagctgcg aggattgcgt
    14881 cggcagacgg cccggaagat cggtataacc aatcactttg acaccatttt ccgtagtgaa
    14941 gatttcaccc ggcacggtgt attcacagtt gccaccgttt tgggctgcca ggtcgacaat
    15001 cacactgccc gtcttcatgg agtcaaccat ttcacgggta attagcttcg gcgctggttt
    15061 gcctggaata agcgcggtgg tgacaatgat atcgacctct tttgcctggg cggcaaagag
    15121 ttccatttct gctttaatga acgcgtccga catcactttg gcatagccat cgccgctgcc
    15181 cgcttcctct ttaaaatcca gctcgaggaa ttccgcgccc atactttgaa cttgttcttt
    15241 cacttccgga cgggtgtcga atgcacgcac aatcgcgccg agactgtttg ctgcgccaat
    15301 ggcggccaga cctgcaacac ccgcaccaat caccatcact tttgccggtg gcactttccc
    15361 ggccgcagta atttgcccgg taaagaagcg cccaaattca tgtgccgctt caacaatggc
    15421 gcgataaccg gcgatgttcg ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat
    15481 acgcggcaca gaatccatcg ccatcacggt cacgttacgt tccgcaagtt tttgcattaa
    15541 ttccggattc tgcgcaggcc agataaaact caccagcgtt gtcccaggat tcagtaacgc
    15601 aatttcatca tctaacggcg cattgacctt cagaatgatc tctgactgcc agacgctatt
    15661 cccttctaca atttcagcgc ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg
    15721 acccgcgccg ctctctaccg cgacggtaaa acccagtttc agcagctgtt ccactgtttt
    15781 tggcgttgct gcaacacggg tttcattggt taaccgttct cttggtatgc caattcgcat
    15841 gatattccct tccatcggtt ttattgatga tggtttgcct gtgtcaggag ccacacaagc
    15901 cgctcatgta cgagctaaat gttactccgt taaaataaat tagtaacaaa cgccctataa
    15961 cgtactgaaa attatgcctg tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc
    16021 aaaatattaa cgattcagcc gtgatagtgg gataaacacc ttagaacgcc ggataaagac
    16081 tgataattgt cttcgacggt cgggtaaaac gagacatcgc cccggcacga ttcactactt
    16141 aacattaaat taacttatac aattcagttg cttcagtagt aatgatgctg atacggctgt
    16201 tttttaagca tagacggtca tttgagcagg attaaaattg gcttaaggaa tgtgatatga
    16261 aaaatgacgc agacagttac accgtttaaa tgcaataatc agccacgttt ctcgttaata
    16321 acaataccag tacctggttt gcgcaaggcg aaggattatt tttatgaagc ttaagaacac
    16381 cctcctggcg tcggcactgc tttctgctac ggcattctcc gttaacgcag caacagaact
    16441 gacaccggag caagcggcag cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt
    16501 taatgctatt ggcgaagcgg tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc
    16561 ctctttttat gttgtcgaca cttctgattt tggtaacagc ggtaactggc gtgtggtcgc
    16621 tgacctctat aaagccgatg ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg
    16681 tgttgtcgaa ctgccgaaag atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt
    16741 ccagggcttc tatcgtagcc agccagaagt caatgatgcc atcaccaaag cggcaaaagc
    16801 gaaaggtgcc tactctttct acatcgttcg tcaaatcgat gccaaccagg gcggcaacca
    16861 gcgtattact gcattcatct ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga
    16921 tgtgatcccg gcagattccg aagcaggacg tgcagctctg gctgccggtg gcgaagccgc
    16981 gaagaaagtt gagatcccag gtgttgcgac taccgcatca ccaagttctg aagtcggtcg
    17041 tttctttgaa acccagtcat caaaaggcgg gcgttacacc gtcacgctcc cggatggcac
    17101 taaagtcgaa gaactgaaca aagcgaccgc agcgatgatg gtcccgttcg atagcatcaa
    17161 attctctggc aactacggca acatgaccga agtctcctat caggttgcga aacgtgccgc
    17221 gaagaaaggt gctaagtatt accacatcac ccgccagtgg caggaacgtg gtaataacct
    17281 gaccgtcagc gcagatctgt ataaataact attcagttac tcctataagg cggcttgatg
    17341 ccgcctttcg catttttagt cacaaatttc cccgcgacca ttgcatgcag acacatctct
    17401 tcgtaaaatc ccgcgcttca gtggcaaact ccatttttat gcgttttgcc ctaatattta
    17461 ttcttcttat cacgttttaa tcactggata tcgatggaaa agaaactggg actgagcgca
    17521 ctcaccgcgc tggtattaag ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat
    17581 atggcggcag ttgccagccc ggcagcactg ctcatcggct ggggtattac tggcgctggc
    17641 attttattgc tggcctttgc catgctgatc ctcacgcgca ttcgtcccga acttgacggc
    17701 ggtatcttta cctatgcccg cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg
    17761 ggatactggc tgtgcgcagt catcgccaac gtctcctatc tggtgatcgt tttttccgcg
    17821 ttaagctttt ttacggacac gccggaattg cgcctgttcg gcgatggtaa tacctggcaa
    17881 tcgatcgtcg gtgcatcggc tttattgtgg attgttcact ttttgatcct gcgcggggtg
    17941 caaaccgctg ccagcattaa cctggtggcg acactggcaa aattgttgcc gctgggtctg
    18001 tttgttgtgc tggcgatgat gatgttcaaa ctggatacct tcaagctcga cttcaccgga
    18061 cttgtacttg gcgtacccgt ttgggaacag gtgaaaaaca ccatgctgat caccctgtgg
    18121 gttttcattg gtgtggaagg tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat
    18181 gttggcaaag cgacactgct ggcggttctc tccgctctgg gcgtttactt gctggtaacg
    18241 ctgctttcgc tgggtgtggt ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg
    18301 gccggtctga tggtggaaat gatgggacca tggggcgaaa tcatcatcgc tgccagtttg
    18361 atcgtttccg tttgcggcgc gtacttaagc tggaccatca tggcggcaga agttccgttc
    18421 ctggctgcca ctcataaagc attcccacgc atttttgcgc gccagaacgc acaagctgcg
    18481 ccatccgcct cactgtggtt gaccaacatc tgcgtgcaaa tttgtctggt actcatctgg
    18541 ctgaccggtt ccgattacaa cacgttgctg accatcgcct cagaaatgat tctggtgccc
    18601 tatttcctgg tcggcgcgtt cttgctgaaa atcgcaaccc gtccactgca taaagcggta
    18661 ggtgtcggtg cctgcattta tggcttatgg ttattgtacg cctctggtcc aatgcacttg
    18721 ctgttatccg ttgttctgta tgcgccgggg ctgctggttt tcctgtatgc gcgcaaaacg
    18781 catacacatg ataatgtact gaaccgtcag gaaatggtgc tgattggcat gctgttaatt
    18841 gcctcagttc cggcgacctg gatgctggtg ggataacgtt catcccatcg ttggatagca
    18901 aaggagataa cgatgggtaa agcccagccc ttgccaatat taattactgg cggaggtcgt
    18961 cgcatcggcc tcgccctcgc ctggcatttc attaatcaaa agcaaccggt gattgtcagc
    19021 tatcggacgc actatccagc cattgatgga ctgattaagg caggtgcgca gtgtattcag
    19081 gctgattttt cgaccaacga cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc
    19141 catggtctgc gtgctatttt gcataacgcc agtgcgtgga tggcggaaaa actgggtgcg
    19201 ccactggccg acgtactggc ttgcatgatg cagatccacg ttaatacccc atacctgctc
    19261 aaccatgcgc tggaaagatt actgcgtggg cacggacacg ccgccagcga tatcattcac
    19321 tttaccgatt atgtggtgga gcgcggtagc gacaagcata ttgcgtatgc tgcaagcaaa
    19381 gcggcactgg ataatatgac ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg
    19441 aattctattg ctccatcgct gatcctgttt aatgaacatg atgatgccga atatcgacaa
    19501 caggcgctga ataaatcact gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg
    19561 gtcgattact tacttaccag ttgctttgtc accggacgca gtttcccact tgatggcggt
    19621 cgtcatctgc gttaatgcag ctttatccag catataatca acacccatgc aacacgcaac
    19681 agaaccaaca aaagcggcgg gcaagcgcat cattccggtg aaataccaca gcgacaccag
    19741 gtaaacaaaa tagggaataa tcgaccacat actaaaaatg atggttgcgc gtaaggcttc
    19801 aatgccgcgt tcgctggcaa caatataatg cgcgataagc gcaaaggtcg ggaaaagtgg
    19861 aatcagcccg gcgatataat aatttttcgt ttttgctaaa acaccaatca acagcactac
    19921 cagcgcaccg agcgcggctt tgattttacc ttaacacttc cataacaagt catcagtaga
    19981 atacctgatg aaaacttgtt tagaaacgat tgatagtaag taaaaacagc gcggtgtatt
    20041 gtgacgtttt tatatctacc gtgaatgtta tgaacactat cgtatttgtg gaagatgatg
    20101 cggaagtcgg ttcactgatt gccgcgtacc tggcaaaaca tgatatgcag gttaccgtag
    20161 agccgcgcgg cgaccaggcc gaagaaacca ttttgcgaga aaatccggat ttggtgttac
    20221 tcgacatcat gctaccaggc aaggacggca ttaccatttg tcgtgattta cgcgcaaagt
    20281 ggtctggacc gattgttctt ctaacctctc tcgatagcga tatgaaccac atcctggcac
    20341 tggaaatggg tgcctgcgac tatattctca aaacgatgcc ccctgctgtt ttgctagcgc
    20401 gtttacgttt gcatttgcgt cagaatgagc aagccacact gaccaaaggt cttcaggaaa
    20461 cgtctctgac tccctacaaa gccctgcatt tcggcacgtt gaccatcgat cccatcaacc
    20521 gcgtagtcac cctggctaac actgaaatct cgctctcgac agctgatttc gaattattgt
    20581 gggaattagc tacccatgcc gggcaaatca tggaccgcga tgcattgctg aaaaatttac
    20641 gcggcgtcag ttatgacgga ctggatcgta acgtggacgt ggctatttcg cggttaagaa
    20701 aaaaactgct cgataacgcc gcagaacctt atcgcattaa aactgtgcgt aacaaaggct
    20761 atctttttgc gcctcatgca tgggaataag cgatgaaaaa actgtttatc cagttttacc
    20821 tgttattgtt tgtctgcttc cttgtgatgt ctctgctggt tgggttggtg tacaaattta
    20881 ccgccgaacg cgcgggcaaa cagtcgctgg atgatttgat gaacagttcg ctgtatctga
    20941 tgcgcagcga attgcgtgag atccccccac acgactgggg taagacgctg aaagagatgg
    21001 atttaaatct ctctttcgat ctgcgtgtcg agccactgag taaataccat cttgatgata
    21061 tttccatgca ccgactgcgt ggcggcgaaa ttgtcgccct ggacgatcag tacacgtttt
    21121 tgcagcgtat cccgcgcagc cactacgtgc tggcagttgg tcctgttcct tatctttatt
    21181 atctccatca gatgcgattg ctggatatcg ccctgatcgc ttttattgct atttccctcg
    21241 cctttccggt gtttatctgg atgcgtccgc actggcagga tatgttaaaa ctggaagcag
    21301 cggcgcaacg atttggcgat gggcatctct atgagcgtat ccactttgat gagggttcga
    21361 gctttgaacg acttggtatc gcatttaacc agatggcgga caatatcaac gccttgattg
    21421 ccagcaaaaa acagcttatt gacggtatcg ctcacgaact gcgaacaccg ttagtgcgcc
    21481 tgcgttatcg actggagatg agcgataacc tgagcgccgc cgagtcccag gcgttgaatc
    21541 gtgatatcag tcaacttgaa gctttaattg aagagctgct gacttatgcc cgactcgatc
    21601 gcccacaaaa cgagcttcat cttagcgaac cagacctgcc gttgtggctg tcaacgcatc
    21661 tggcagatat tcaggcagta acgcccgata aaacggtacg gataaaaacg ctcgtgcaag
    21721 gccattatgc ggcgttggat atgcgcttaa tggagcgcgt gctggataat ttgctcaata
    21781 acgccctgcg gtactgccat tcaacggttg aaaccagcct gctactgtcg gggaatagag
    21841 cgacattaat tgttgaggat gatggcccag ggattgcccc agaaaaccgc gaacatatct
    21901 ttgaaccttt tgttcgcctc gatcccagcc gggatcgctc aaccggcggc tgcgggctgg
    21961 ggctggcaat tgtccactct atagcactgg caatgggcgg tacggttaat tgtgacacca
    22021 gcgaactggg tggtgctcgc ttctcgttta gctggccgtt atggcataac atcccgcaat
    22081 ttacctctgc ctgacactac gcgcacgatg gtcaagtcac caccattgtg ctataaatta
    22141 aggatgttgt aactaaagtg gttaatatta tggcgcgtta cgatctcgta gaccgactca
    22201 acactacctt tcgccagatg gaacaagagc tggctgcatt tgccgctcat cttgagcaac
    22261 acaagctatt ggttgcccgc gtgttctctt tgccggaggt aaaaaaagag gatgagcata
    22321 atccgcttaa tcgtattgag gtgaaacaac atctcggcaa cgacgcgcag tcgctggcgt
    22381 tgcgtcattt ccgccattta tttattcaac aacagtccga aaatcgcagc agcaaagccg
    22441 ctgtccgtct gcctggcgtg ttgtgttacc aggtcgataa cctttcgcaa gcagcgttgg
    22501 tcagtcatat tcagcacatc aataaactca agaccacgtt cgagcatatc gtcacggttg
    22561 agtcagaact ccccaccgcg gcacgttttg aatgggtgca tcgtcatttg ccggggctga
    22621 tcacccttaa tgcttaccgc tcgctcaccg ttctgcacga ccccgccact ttacgttttg
    22681 gctgggctaa taaacatatc attaaaaatt tgcattgtga tgaagtcctg gcacagctgg
    22741 aaaaaagcct gaaatcacca cgcagtgtcg caccgtggac gcgcgaggag tggcaaagaa
    22801 aactggagcg agagtatcag gatatcgctg ccctgccaca gaacgcgaag ttaaaaatca
    22861 aacgtccggt gaaggtgcag ccgattgccc gcgtctggta caaaggagat caaaaacaag
    22921 tgcaacacgc ctgccctaca ccgctgattg cactgattaa tcgggataat ggtgcgggcg
    22981 tgccggacgt tggtgagttg ttaaattacg atgccgacaa tgtgcagcac cgttataaac
    23041 ctcaggcgca gccgcttcgt ttgatcattc cacggctgca cctgtatgtt gcagattaac
    23101 gcccggcttt catactgccg accatctgtt ctggccgtac ccagctgtca aactcggctt
    23161 cgctaagata ccccagcgca agggccgcag cttttaaggt cagcccttct ttatgcgctt
    23221 ttttggcgat ctccgcggct ttgtcataac caatgtgggt gttaagcgca gtcaccagca
    23281 tcagcgattc attgagtaat tgattgattc gctcacgatt cggttcaata cccactgcgc
    23341 agtgtttgtt aaaactttcc atgccatctg ccagcaagcg caccgattgc aggaaattat
    23401 ggatcaccat cggacggaag acgttcagtt caaagttacc ggaagcgcca cccatgttga
    23461 tcgccacgtc gttccccatt acctgacagc agagcatggt taatgcttca cactgggttg
    23521 ggttcacttt ccccggcatg attgagctgc ccggctcatt ttccgggatt gagatttcac
    23581 caattccgca gcgcgggcca gaggccagcc agcggacatc attggcgatt ttcatcagtg
    23641 acgcagccaa ccctttcaac gcgccgtgcg cctgaaccag ggcatcacag gtcgcaagcg
    23701 cttcaaattt gttcggcgcg gtaacaaacg gtgcacaggt aatgactgcc agttcatctg
    23761 ctacgcgacg cgcatactcc ggatgggtat ttagtccagt acccaccgct gtaccgccaa
    23821 gagccagttc cgctacgtga ggcaggctgt attcgatatg tttgagatta tgctcgagca
    23881 tcgctaccca gccggaaatc tcctgcccta gtgttagcgg cgtggcgtcc tgcaagtggg
    23941 ttcgaccgat tttgacgata tcggcaaatg cacgcgattt ttcactcagt gtctgcgtca
    24001 gggttttaag ctgcggaatg agttgcttgc gcagcgccag cagcgccgca acgtgcatcg
    24061 ccgtcggaaa gacatcgttg gaactttggc ttttgttcac gtcgtcgtta gggtgaactt
    24121 tacgttccat cccgcgcacg ccgccgagta attcactggc ccggttagcc agcacttcgt
    24181 tcatgttcat gttactttgc gtgccggagc cggtctgcca gatagccagc gggaattcgt
    24241 cgtcatgctg tcctgccagt acttcatccg ccgcctgccg aatggcgctc gctttctctt
    24301 cagacaacaa gcctaaatct tcattaactt ttgccgctgc gcgcttggtt agcgccagcg
    24361 catgaatcag tgaggtgggc attttctccg tcgaaatgcg gaaatgctcc agcgagcgtt
    24421 gagtttgtgc gccccacagc ttatctgccg ggacatcaat cgcccccatc gaatcttttt
    24481 cgctgcgtac tgtattcatg gcctgctcct cacctgttta attttttctt tctgttttgc
    24541 tttcgctaag caacttttag ctcacttatt atttactatt tgataacaag tgtttggtct
    24601 ttcgtgccat gtaaaaaaac cgccccgaag gg
//
LOCUS       DAJTOR010000046        24496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTOR010000046 DAJTOR010000000
VERSION     DAJTOR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.046"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..94)
                     /locus_tag="OO249_001670"
     CDS             complement(<1..94)
                     /locus_tag="OO249_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5636011.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNK"
     gene            complement(353..1234)
                     /gene="yddG"
                     /locus_tag="OO249_001671"
     CDS             complement(353..1234)
                     /gene="yddG"
                     /locus_tag="OO249_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5636012.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEAGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1466..4513
                     /gene="fdnG"
                     /locus_tag="OO249_001672"
     CDS             1466..4513
                     /gene="fdnG"
                     /locus_tag="OO249_001672"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2051..2053,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5636013.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGERVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4526..5410
                     /gene="fdnH"
                     /locus_tag="OO249_001673"
     CDS             4526..5410
                     /gene="fdnH"
                     /locus_tag="OO249_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5636014.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEAPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            5403..6056
                     /gene="fdnI"
                     /locus_tag="OO249_001674"
     CDS             5403..6056
                     /gene="fdnI"
                     /locus_tag="OO249_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5636015.1"
                     /translation="MSKSKMIVRTKFIDRVCHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(6107..6391)
                     /locus_tag="OO249_001675"
     CDS             complement(6107..6391)
                     /locus_tag="OO249_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5636016.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(6537..7547)
                     /gene="adhP"
                     /locus_tag="OO249_001676"
                     /pseudo
     CDS             complement(6537..7547)
                     /gene="adhP"
                     /locus_tag="OO249_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            complement(7681..9378)
                     /gene="maeA"
                     /locus_tag="OO249_001677"
     CDS             complement(7681..9378)
                     /gene="maeA"
                     /locus_tag="OO249_001677"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5636017.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(9535..9672)
                     /gene="sra"
                     /locus_tag="OO249_001678"
     CDS             complement(9535..9672)
                     /gene="sra"
                     /locus_tag="OO249_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR5636018.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(9774..9989)
                     /gene="bdm"
                     /locus_tag="OO249_001679"
     CDS             complement(9774..9989)
                     /gene="bdm"
                     /locus_tag="OO249_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR5636019.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10335..10766
                     /gene="osmC"
                     /locus_tag="OO249_001680"
     CDS             10335..10766
                     /gene="osmC"
                     /locus_tag="OO249_001680"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5636020.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(10822..11748)
                     /locus_tag="OO249_001681"
     CDS             complement(10822..11748)
                     /locus_tag="OO249_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5636021.1"
                     /translation="MSDTLLTLRGVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALVEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(11741..12727)
                     /locus_tag="OO249_001682"
     CDS             complement(11741..12727)
                     /locus_tag="OO249_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5636022.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVRADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(12724..13620)
                     /locus_tag="OO249_001683"
     CDS             complement(12724..13620)
                     /locus_tag="OO249_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5636023.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPRAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(13617..14639)
                     /locus_tag="OO249_001684"
     CDS             complement(13617..14639)
                     /locus_tag="OO249_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5636024.1"
                     /translation="MTFWSILRQRCWGLVLVMAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(14641..16191)
                     /locus_tag="OO249_001685"
     CDS             complement(14641..16191)
                     /locus_tag="OO249_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5636025.1"
                     /translation="MKRSIPFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLA
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDKDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(16205..16786)
                     /gene="ddpX"
                     /locus_tag="OO249_001686"
     CDS             complement(16205..16786)
                     /gene="ddpX"
                     /locus_tag="OO249_001686"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR5636026.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(17044..19443)
                     /gene="dosP"
                     /locus_tag="OO249_001687"
                     /gene_synonym="pdeO"
     CDS             complement(17044..19443)
                     /gene="dosP"
                     /locus_tag="OO249_001687"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR5636027.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNHDYL
                     LSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(19468..20849)
                     /gene="dosC"
                     /locus_tag="OO249_001688"
                     /pseudo
     CDS             complement(19468..20849)
                     /gene="dosC"
                     /locus_tag="OO249_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            complement(21221..22540)
                     /locus_tag="OO249_001689"
     CDS             complement(21221..22540)
                     /locus_tag="OO249_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5636028.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(22671..24206)
                     /gene="gadC"
                     /locus_tag="OO249_001690"
     CDS             complement(22671..24206)
                     /gene="gadC"
                     /locus_tag="OO249_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5636029.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         6109 a         6177 c         6179 g         6031 t
ORIGIN      
        1 gtttattacc atccttgtta tagacttcgg cagcgtgtgc tacgttcgca gctaacaaac
       61 cagttacaac caccgcaact atttttaatt tcatttaaca gaatcctttt aattatcgtc
      121 aaacgtattt tctaaacgaa ttttaaacgg cgtcatttat aaatgacata ctgtttttaa
      181 aatagaatgg tgattaaaaa caatgagaaa aatagatgag ataatattat ttatcgatat
      241 gtgatcgaag tcgaaatgag atataaggtg aattactggt atttgaaatt tattttttaa
      301 tattgtcgga atttatctga ttaactgccg ggccgtagac ccggcaatta ttttaaccac
      361 gacgtgtcgc cagccagcag agcagggaac cgccgcagac cattagcgcg ccttgccaga
      421 atgagaacga cagcggggcg ctgagcagca cggctgcaag cgctgaggaa agtacgggcg
      481 taaaatacga gcctaccgcc attatggtga cattgccatg caatataccg acattccatg
      541 cagcataagc aaatcctaag gtaaatgccg cagagatgag tttaatcatg acgggcgtgc
      601 taaataccat ttctggttgt tgcgtaagaa aatagtaaac ccacagactt gctcccgtta
      661 gcaggacaaa aacggtaatt ccgttaaatc cgcgtgcgta tttattcgtt actgtgcaat
      721 aggctgccca gataaacgca ccaataaacg ccaggaaata actcaatggg ctggtggtga
      781 tattattgat gatttcatca taatgtaacc cattctcacc gcctaacacc cagcagacgc
      841 cgaccagggc taataataat cctggtacaa tcagccagtt ggttttctga ccattaaaca
      901 gaatggcaaa gagaattgtc aggctgggcc acagatagtt caccataccc gcttcaatcg
      961 cctgatgacg ggtcgccgca taccctaagg aaagcgccag acagatttca tagctgacga
     1021 ataacagact cccggcgagt aaatagcctt tcggaatctg ccgaatacgc ggaaatccaa
     1081 ccgtgaagat taacagcagc ccgcttaatg aatagatagc agctgcgccg ccgaccgggc
     1141 cgagcccctc gctgacaccg cgaatcaatc ctaccatcgt gctccacagg acgatcgcta
     1201 ttagccctat gagcgttgct ttttgtcgtg tcatgctcgc tgttttgtct ctcttgccgt
     1261 taaaaattaa gctgaatttt atagcatttt tttaactggc ctgtcaggca gtggtgcgtt
     1321 tttctaccgc tattgaggta ggtcaatttg cgcaggcgga ttattttgtg gcaaacacgt
     1381 gttctttttg atttcgcgca aaaagattca gaattttact gttagtttcc tcgcgcagta
     1441 atatccctga aaaaagagga aagcaatgga cgtcagtcgc agacaatttt ttaaaatctg
     1501 cgcgggcggt atggctggaa caacggtagc ggcattgggc tttgccccta agcaagcact
     1561 ggctcaggcg cgaaactaca aattattacg cgctaaagag atccgtaaca cctgcacata
     1621 ctgttccgta ggttgcgggc tattgatgta tagcctgggt gatggcgcga aaaacgccag
     1681 agaagcgatt tatcacattg aaggtgaccc ggatcatccg gtaagccgtg gtgcgctgtg
     1741 cccaaaaggg gccggtttgc tggattacgt caacagcgaa aaccgtctgc gctacccgga
     1801 atatcgtgcg ccaggttctg acaaatggca gcgcattagc tgggaagaag cattctcccg
     1861 tattgcaaag ctgatgaaag ctgaccgtga cgctaacttt attgaaaaga acgagcaggg
     1921 cgtaacggta aaccgttggc tttctaccgg tatgctgtgt gcctccggtg ccagcaacga
     1981 aaccgggatg ctgacacaga aatttgcccg ctccctcggg atgctggcgg tagacaacca
     2041 ggcgcgcgtc tgacacggac caacggtagc aagtcttgct ccaacatttg gtcgcggtgc
     2101 gatgaccaac cactgggtgg atatcaaaaa cgctaacgtc gtaatggtaa tgggcggtaa
     2161 cgctgctgaa gcgcatcccg tcggtttccg ctgggcgatg gaagcgaaaa acaacaacga
     2221 tgcaaccttg atcgctgtcg atcctcgttt tacgcgtacc gcttctgtgg cggatattta
     2281 cgcacctatt cgttccggta cggacattac gttcctgtct ggcgttttgc gctacctgat
     2341 cgaaaacaac aaaatcaacg ccgaatacgt taaacattac accaacgcca gcctgctggt
     2401 gcgtgatgat tttgctttcg aagacggcct gttcagcggc tacgacgctg aaaaacgcca
     2461 gtatgataag tcgtcctgga actatcagtt cgatgaaaac ggctatgcaa aacgcgatga
     2521 aacactgact catccgcgct gtgtgtggaa cctgctgaaa gcgcacgttt cccgctacac
     2581 gccggacgtc gttgaaaaca tctgcggtac gccaaaagcc gacttcctga aagtgtgtga
     2641 agtgctggcc tccaccagcg caccggatcg cacaaccacc ttcctgtacg cgctgggctg
     2701 gacgcagcac accgtgggtg cgcagaacat ccgtactatg gcgatgatcc agttgctgct
     2761 cggtaacatg ggtatggccg gtggcggcgt gaacgcattg cgtggtcact ccaacattca
     2821 ggggctgact gacttaggtc tgctctctac cagcctgcca ggttatctga cgcttccgtc
     2881 agaaaaacag gttgatttgc agtcgtatct ggaagcgaac acgccgaaag cgacgctggc
     2941 tggtcaggtg aactactgga gcaactatcc gaagttcttc gttagcctga tgaaatcttt
     3001 ctatggcgat gccgcgcaga aagagaacaa ctggggctat gactggctgc cgaagtggga
     3061 ccagacctac gacgtcatca agtatttcaa catgatggac gaaggcaaag tcaccggtta
     3121 tttctgccag ggctttaacc cggttgcgtc cttcccggac aaaaacaaag tggtgagctg
     3181 cctgagcaag ctgaagtaca tggtggttat cgatccgctg gtgactgaaa cctctacctt
     3241 ctggcagaac cacggcgagt cgaacgatgt cgatccggcg tctattcaga ctgaagtatt
     3301 ccgtctgcct tcgacctgct ttgccgaaga agatggttct atcgctaact ccggtcgctg
     3361 gttgcagtgg cactggaaag gtcaggacgc gccgggcgaa gcgcgtaacg acggcgaaat
     3421 tctggcgggt atctaccatc atctgcgcga gctgtaccag gccgaaggtg gtaaaggcgt
     3481 agaaccgctg atgaagatga gctggaacta caagcagccg cacgaaccgc aatctgacga
     3541 agtggctaaa gagaacaacg gctacgcgct ggaagatctc tatgacgcta atggcgtgct
     3601 tattgcgaag aaaggtcagt tgctgagtag ctttgcgcat ctgcgtgatg acggtacaac
     3661 cgcatcttct tgctggatct acaccggtag ctggacagag cagggcaacc agatggctaa
     3721 ccgcgataac tccgacccgt ccggtctggg gaatacgctg ggatgggcct gggcgtggcc
     3781 gctcaaccgt cgcgtgctgt acaaccgtgc ttcggcggat atcaacggta aaccgtggga
     3841 tccgaaacgg atgctgatcc agtggaacgg cagcaagtgg acgggtaacg atattcctga
     3901 cttcggcaat gccgcaccgg gtacgccaac cgggccgttt atcatgcagc cggaagggat
     3961 gggacgcctg tttgccatca acaaaatggc ggaaggtccg ttcccggaac actacgagcc
     4021 gattgaaacg ccgctgggca ctaacccgct gcatccgaac gtggtgtcta acccggttgt
     4081 tcgtctgtat gaacaagacg cgctgcggat gggtaaaaaa gagcagttcc cgtatgtggg
     4141 tacgacctat cgtctgaccg agcacttcca cacctggacc aagcacgcat tgctcaacgc
     4201 aattgctcag ccggaacagt ttgtggaaat cagcgaaacg ctggcggcgg cgaaaggcat
     4261 taataatggc gagcgtgtca ctgtctccag caagcgtggc tttatccgcg cggtggctgt
     4321 ggtaacgcgt cgtctgaaac cgctgaatgt aaatggtcag caggttgaaa cggtgggtat
     4381 tccaatccac tggggctttg agggtgtcgc gcgtaaaggt tatatcgcta acactctgac
     4441 gccgaatgtc ggtgatgcaa actcgcaaac gccggaatat aaagcgttct tagtcaacat
     4501 cgagaaggcg taagggggcg aacagatggc tatggaaacg caggacatta tcaaaaggtc
     4561 cgcaactaac tccatcacgc cgccttctca ggtgcgtgat tacaaagcag aagtcgcaaa
     4621 acttatcgac gtttccacct gtatcggctg taaagcctgt caggtggcgt gttcggagtg
     4681 gaacgacatc cgtgatgaag tggggcactg cgtcggggtt tacgataacc ccgccgatct
     4741 gagcgccaag tcctggacgg tgatgcgctt tagcgaaacc gaacagaacg gcaagctgga
     4801 gtggctgatc cgtaaagacg gctgtatgca ctgtgaagct cccggctgcc tgaaggcgtg
     4861 cccgtctgct ggtgcaatca ttcagtacgc taacgggatt gtcgatttcc agtcggaaaa
     4921 ctgcatcggc tgtggttact gcattgccgg gtgtccgttt aatattccgc gcctcaacaa
     4981 agaggataac cgggtatata aatgcacgct ctgcgtcgat cgcgtcagcg tcggccagga
     5041 accggcttgt gtgaaaacct gtccgaccgg ggctatccac ttcggcacca agaaggagat
     5101 gctggagctg gcggaacagc gcgtggcgaa actgaaagcg cgtggttacg aacatgctgg
     5161 cgtctacaac ccggaagggg tcggtggtac gcacgttatg tacgtgctgc atcacgccga
     5221 tcagccggag ctgtatcacg gtctgccgaa agatccgaag atcgacacct cggtaagcct
     5281 gtggaaaggc gcgttgaaac cgctggcagc ggctggcttt attgccactt ttgccgggtt
     5341 gattttccac tacatcggta ttggcccgaa taaggaagtg gacgatgacg aggaggatca
     5401 tcatgagtaa gtcgaaaatg attgtgcgca ccaaatttat tgatcgcgtc tgtcactgga
     5461 ccgtggtgat ttgcttcttc ctggtggcgc tgtccgggat ttcgttcttc ttcccgacgc
     5521 tgcaatggct gacgcaaacc ttcggtacgc cgcagatggg acgcattttg cacccgttct
     5581 tcggcattgc gattttcgtc gcgctgatgt ttatgtttgt gcgttttgtg catcacaaca
     5641 tcccggataa gaaagatatt ccgtggctat tgaacattgt cgaagtatta aaaggcaatg
     5701 agcacaaagt ggcggatgtt ggtaagtaca acgccgggca aaagatgatg ttctggtcga
     5761 tcatgagcat gattttcgtg ctgttggtga ccggggtgat tatctggcgt ccgtactttg
     5821 cgcagtactt cccgatgcag gttgttcgct acagcctgct gatccacgcg gctgccggta
     5881 tcatcctgat ccacgccatc ctgatccata tgtatatggc attctgggtg aaaggatcga
     5941 ttaaagggat gatcgaaggg aaggtaagcc gtcgctgggc gaagaaacac catccgcgct
     6001 ggtatcgtga aatcgagaag gcagaagcga aaaaagagag tgaagaaggg atataatctc
     6061 ttttgaactt taagctgaaa atggcgcttt aagaagcgcc atttttttat tgcgtaacca
     6121 gacggcgcag tcgcgacaca tccaccgttt tttcagcctc tgccagactc caggcgtttt
     6181 gcagattcag ccacatttgc ggcgaactgc cgatcaccac ggaaagttta attgccattt
     6241 ctggcgtcaa agctgctttt ccggtcagca atcggcttgc cgttgagggc gcaatttcca
     6301 ttgctctggc aaactcgcgc aggctgacat taagttcgtc cagtgattcc tgaataatgt
     6361 cccccgggcg gggatgattg gccattttca ttagtgtctt tactctcagt agcgttcctt
     6421 ctctgggaac actatcatta ttgttccttc ctacgcaaca ataatccatt tagccatttc
     6481 atgttggaga gtgtgcgatg ctgcgacccg aacatgggag tcgcagcaaa gacctcttag
     6541 tgacggaaat caatcaccat acggccacgg attttgcctt cttccatctc ggtaaagatg
     6601 gtgttgatgt ccgctaacgg acgcagggcg actttcggca ccactttgcc ttcggcggca
     6661 aactggaagg cttcggttag atcctggcgc gtgccgacca gcgaaccgac cacttcaatg
     6721 ccatccagca caagacgtgg gatatccagg ctcatagact ccggcggcag accgacagcc
     6781 acaacacgac cgcctgcacg gacagcatca actgccgagt taaacgcagc tttagctact
     6841 gctgttacca ccgcagcgtg agcgccaccg gttttctcct gcacaattta ggcggcgtct
     6901 tcggtgcgtg agttaatcgc taaatctgca cccatttcgg ttgccagttt taactgctca
     6961 tcattgacat caatggcgat cactttggcg ttaaagacat tcttcgcgta ttgcagggcg
     7021 aggttaccca gaccgccaag accgtagata gcaatccact gaccgggacg aatttttgac
     7081 agtttaacgg ctttgtaggt ggtgaccccc gcacaggtaa tgctgctggc cgccgccgag
     7141 tccagaccat ctggcacttt taccgcgtaa tcggcgacca cgatgcactc ttccgccatc
     7201 ccgccatcaa cgctgtatcc ggcattttta actgaacggc agagcgtttc gttaccactg
     7261 ttacagtatt cgcaatgacc gcatccttcg taaaaccacg ccacgctggc acgatcgcct
     7321 ggttttaatg aggtgacacc tgggccaact tctgccacca caccgatgcc ttcatggccc
     7381 agaattaccc cagttttgtc accaaaatcg ccattcttaa catgaagatc ggtatgacat
     7441 acaccacaac actccatttt cagcagggct tcgccatgtt tcagtgagcg cagtgtttta
     7501 tccgtaacgt caacatgatg atccttcgta acaactgcag ccttcatagt tcctcctttt
     7561 cggatgatgt tctgcatagc aggtgaggca aatgagattt attcgccact acccagtatg
     7621 gatgagatct gaaaaaggga gagggaaata gcccggtagc cttcactacc gggcgcaggc
     7681 ttagatggag gtacggcggt agtcgcggta ttcggcttgc cagaaattat cgtcaatggc
     7741 ttgttgcaaa gcttcagcag acgttttcac cgccacgcct tgctgctgcg ccattttgcc
     7801 aaccgcaaac gcaattgcgc gggagacttt ctgaatatct ttcagttccg gtagtaccag
     7861 accttcgccg ttcagcacca gcggcgaata ctgagcaagc gtttcacttg ctgacatcag
     7921 catctcatcg gtgatacgtg acgcgccgga agcaataaca cccagcccga tgccagggaa
     7981 aataaaggcg ttgttacact gggcgatagg gtagatttta tctttccata ccactggatt
     8041 aaacgggctg ccagtggcga ccagcgcgtt accttcggtc caggcgataa tgtcctgcgg
     8101 tgtggcttcc acgcgtgacg tcgggttaga cagcggcatc acgatcggac gcggacagtg
     8161 tttatgcatc tcacggatga tctcttccgt aaacagcccg gtctgtcctg agacgccaat
     8221 cagaatatct ggttttacat tgcgcaccac atccagcagt gacagcacat cgctgtcggt
     8281 atcccagtca ctgaggtttt cgcgtttctg caccagtttg gtctggaaag gcagcaggtt
     8341 cggcatcttg tccgtcagca ggccaaagcg atcgaccata aagactttct gccgcgccgc
     8401 ttcctcgctt aatccttcgc gctgggtctg ggcgatgatc atttcggcaa tgccgcatcc
     8461 cgctgaacct gcaccaagga agacgatttt tttctcgctt aactgaccac ctgccgctcg
     8521 gcttgctgcg atcagtgtgc cgactgttac cgccgcagtg ccctgaatgt catcgttaaa
     8581 agaacaaatt tcattgcgat agcggttaag taacggcatc gcattttttt gagcaaagtc
     8641 ttcaaactgc aacagcacgt ctggccagcg ttgtttcaca gcctggataa attcatcaac
     8701 gaattcatag tattcgtcgt caatgatacg cggattacgc cagcccatat acagcggatc
     8761 gttaagcagc tgttggttgt tcgttccgac atccagcacc accggaaggg tatacgccgg
     8821 gctgatgccg ccacaggcgg tatagagcga cagtttaccg atcggaatgc ccatcccgcc
     8881 gatgccctgg tcaccaagcc ccagaatacg ttcaccgtca gtcaccacaa tcactttgat
     8941 attatggttc ggtacgtttt gcagaatatc gtccatattg tggcggttct ggtaagagat
     9001 aaacacgccg cgtgaacggc ggtagatctc agaaaaacgc tcacaggctg cgccgacggt
     9061 tggggtataa ataacaggca tcatctcatc aagatgattg tttaccagac ggtagaagag
     9121 ggtttcgttg gtgtcctgga tgttacgcag gtagatgtgt ttgtcgattt cggttttgaa
     9181 tccctgatac tggatccatg ctcgttccgc ttgttcttcg atggtttcga ccacttccgg
     9241 cagtaacccc agcaggttga agttacggcg ttcttccatg ctgaaggcac tgcctttatt
     9301 caacaacgga aattccagca gtacagggcc agcgtaaggg atataaagcg aacgctgttt
     9361 ttttgttttt ggttccatgt cactcactct tttttgaata tccatccctg gcacgctttt
     9421 atcgtctttg ctttaccgcc agggcgtcgg ccccaagtat aaagcagata aaaacaaaaa
     9481 caccattgcg caggcaatgg tgtttaatcg tcattgagga ctgatggtta tgaattactt
     9541 ttcagcgggg cgttttctgc cggttgggtt atttaccacg ctggatttgt caccttcggt
     9601 aactattttg cgctggttag aaattttatg gtccagtcca agaatatgac gtgcctgacg
     9661 gttcgatttc attttaactc ctcaatcctg ttgctagttt taaggacaac atcgccgtag
     9721 cgaagaaaca cgtgctaaac ccctaaatta ggttgccgat caagcatagc accttaaagc
     9781 gtagggtgct ggccactgac cacataattg atcgtttgct ggtagatatc actgaggatg
     9841 tcgttatcag aagcttccac ccatttggtc agctccatga gaatgtcatc ttcagtgaca
     9901 acaccgtgct ctgcccgaag gccttgctca atggcattaa ccagagcggg ttctgctgtt
     9961 gaattttctg cctgataata agtaaacata gtgattctcc gtgtctgtgt atttatggtg
    10021 tctgctacgg atcgcagatt tataaagcac attcagcatg gcaaatattt gccgctttgt
    10081 tgttaagatt agtcctggtt gatgattttt atattttaac ctcatgatat tgataggtat
    10141 tattattgtg gtgagttgat taatattaat gcaatatctc ttgggttata tattcccggt
    10201 tatctattgt ggaaatttaa tttaagtgca gaagtaatat ttctgccgga tttttattcg
    10261 gaatatcctg cttatcctcg tgctgtttct cacgtagtct ataatttcct ttttaagccc
    10321 acatgagagc aacaatgaca atccataaga aaggtcaggc acactgggaa ggcgatatca
    10381 aacgcgggaa gggaacagta tccaccgaga gtggcgtgct gaaccaacag ccgtatggat
    10441 ttaacacgcg ttttgaaggc gaaaaaggaa ctaaccctga agaactgatt ggcgcagcgc
    10501 atgccgcatg tttctcaatg gcgctttcat tcatgctggg ggaagcggga tttacgccaa
    10561 catcgattga taccatcgcc gatgtgtcgc tggataaagt ggatgccggt tttgcgatta
    10621 cgaaaatcgc actgaagagt gaagttgcgg tgccgggtat tgatgcctct acctttgacg
    10681 gcatcatcca gaaagcaaaa gcaggatgcc cggtctctca ggtactgaaa gcggaaatta
    10741 cgctggatta ccagttgaaa tcgtaaagca ttgccggatg acgtgtcagg cgcgtgaatg
    10801 cctgatgcgt tgttagcatc tctacagcgc ccgccagcac cggagctcac gtccgtcctc
    10861 ccttattgct aatgattgcc ggacttcaca tccgctggtt gcaagggggc aacgttcgta
    10921 gaaaaaacag ccttgcggca acgtgcggtt tcccggcaga tccgttttac gtaatgccca
    10981 ttcttcctcc agcggtttat caatggcggg gagggaatcc agcaataatc gggtgtatgg
    11041 atgtgccgga gccgttagca cctgttgcgc gtcgcccagt tctacaatct gcccgagata
    11101 catcaccgcc acccgatcgc tcatatgacg tatcaccgag acattgtgtg aaatcagcac
    11161 ataggtcagc ccgtgatttt cctgtagcgt taccagtaaa ttgaggatct gcgcctgcac
    11221 ggagatatcc agcgcagagg ttggctcatc aagcacaatc acgtcaggct gcgaagagag
    11281 tgctctggca atggcgatac gttgccgctg cccgccggag aacgcatgag gcaggcggtc
    11341 gagatactcc ggacgaatac ccacctgcac agccagctcc tctaccaacg ctcgccgctg
    11401 ttgttcacta ctacgcttag ctatccagag cggttctgtg atgatgcgcc acaccggtaa
    11461 gcgcggattg agcgaagaga gcgggtcctg aaacaccatc tgcataatgc gttgtgagcc
    11521 agaacggatg tactgcccgt ggctcggttg cagcataccc attaaaagct gtgcgagggt
    11581 gcttttgccg cagcctgact cgccgacgat ccctaaggtt tcaccacggc ggatctgtaa
    11641 atcaatacca ttaatcgcat gaacatgttc cgtagtttta ccaagccagt ttttacgggc
    11701 cgggaaattg atatggacgc cgcgtaacgt taataacgtg tcagacactt atgacctcct
    11761 gctgcggata ccaacaggcg cagcgctggt tgttgtcacc acacgctgtc agcgccggga
    11821 cgttttcaca ctgtgcacct gccgcatagc aacgatcgcg aaaagcgcag ccgtcaggca
    11881 aatgggtgag gtttggtacc gtccctggaa tggcgggtaa tagctggcgt ggtactccat
    11941 gttctggtgc acattggagc aaaccaatgg tatacggatg ccggggatga tggataacgt
    12001 ctgccctcac gccgctttca attacgcttc cggcatacat cacgtaaacg ctatcgcaca
    12061 gttgcgacac cacggccata tcatggctga tgaacagaac cgcagttcca ctggcgcggg
    12121 ctttatgttt aagcaaacgc agtacctgca actgcaccgt gacgtccagc gccgtagtcg
    12181 gttcgtcggc aataatcaat tgcggctcgc aggagaatgc cagcgcaatc attacccgct
    12241 ggcgcatacc acctgaaagc tcaaacggat agcgcgacat aacttccacg gcatccggga
    12301 tttgcatctc ttccagcagg acaatcgctt tagctctggc ttcccgacga cttattggtt
    12361 gatgatggcg gatcacgtcc atcatctgaa gacctattcg acgtgtcgga ttgagggcgg
    12421 tcatcggttc ctgaaagatc atcgccactc gtgcgccgcg ccactgacga agctgttttt
    12481 cccgagcgtt aagaacatcg tctcccagca gtgaaatctg cccccgatgt acgcaataac
    12541 tgcccgtcgg tagcagacgc ataatcagca ttgcggtgac tgatttacct gagccggatt
    12601 ctcccaccag accgacaatt tcaccgcggt taatctgcaa ggacacattg ttgagcgcgt
    12661 gaacatcacc gttaaaaccg gggaaactca aatgcagctg ttgaatgtcc agaacgggct
    12721 gggtcatgac tgctttcctc ctgctttcgg gtcaagcaga tcgcgaatac catcaccaaa
    12781 gagattaaac ccaacggcgg taagcaaaat cgctgccctt ggaaatgcgc aataccacca
    12841 ctgatcgagt acatagttgc gaccattcgc taccatcgcg ccccattcag cactcggttg
    12901 ttgagcaccc aggccaataa atcccaacgt ggcggccatt aaaatcgcgc taccgatatc
    12961 cagcgatgcc tgcacgatca gcggcggcag ggagttacgt aaaatatgcc agctgatcag
    13021 atgccaacgc gacgcaccaa aggttttcgc cgcctgaaca taggtatact ggcgtacaac
    13081 taatgcttgc ccccgcgcca ggcgcacata aaagggaatt cgcacaatag caatcgccag
    13141 catggcgtta aataaactcg gcccgagagc ggcagccagt gccattgtca gtaccaacga
    13201 aggaatcgac agcataatgt ccatgatgcg catgatgatg gcgtctgcgc gtccgccaag
    13261 cacaccggat agacatccga gtagcgaacc aatcataccc gcaatggcaa ccacgactaa
    13321 tccggcgaga attgattgct gactgccgac cagtacgcgg ctgaacagat cgcgtcccac
    13381 ttcatcggtg ccaaaccagt gcgccgcaga aggcggcaaa aggcgggcgg ttaaatcaat
    13441 ggcgttggga tcatgcgtcg caagccacgg tgaaaaaaac atcatcaata gcattaatac
    13501 aatgatgact gcgccggtca cggtgagcgg actgcctttc agcatccata ccagttttgc
    13561 accgttaaat cgcgtttgtt tttgtgggcg tacggcggac gtttcctcgc ttagcatcat
    13621 tcaccacctc cacgtccgat acgcggatca atccacaaat agagcaaatc caccaccagg
    13681 ttgaccagca cataagcaaa tgaaaccacg acggcaaagc ccatcactgc cgggaagtcg
    13741 agcgcctgta ttgatgttac tacccatgca cccattccgg gccaggcaaa aacggtttcg
    13801 gtgagcactg cgccatacaa caaatcgccc agcgccagac ccaatacggt aatcgatggg
    13861 atcaacgcat tgggtagcgc ataacataaa acgatatacc agccgggcaa gccgctggcc
    13921 cgggcggtac gaatgtagtc ttcactcaat tgttccagca tcgctgagcg gatctggcga
    13981 gcgacaattc ccaggtgaac gaacgccagc gttaatgccg gtaagatgag atgttgcaac
    14041 gcattgaaga agacttcacc gttgccttca agcagcgcat cgagcagata aaagccggta
    14101 acgtgcgttg gtggatccag ccagtcatca agccttccgc cgccgggaag aatttgcaga
    14161 tgaccataaa acagcacaat gacgcccagt ccaagccaaa acgcaggtgt ggagattccg
    14221 gtaatggcca ttattcgcac cagatgatcc agccagcgat ttcgccagac tgcagagagt
    14281 atgcccagcg ggatgccaat caggagtgcc agcagcaggg cacaaaaagc cagttccagc
    14341 gtcgccggga aaaatatacg caactcttcc aacaccggac gcccggtacg aatggatgtt
    14401 cccaggtcac catgaaacag gtcgctgacg tagcggtaaa actgtacata cagtggctgg
    14461 tccagtccca gttgctggcg aatattttcc acgatagcat cgctggcgcg gtcacccgcc
    14521 agtaaccgcg ccggatcgcc agggatcaga tgcgagataa taaacgtaat cacgcagacg
    14581 cccgccatca cgagcaccag cccccagcag cgttggcgta aaatactcca gaaggtcacg
    14641 ttatttactc atggtattga tattgaagac ctgttccagc atgggattga acacaaagcc
    14701 tttcacctct ttgttcatcg ccagttggta gtttttctgg aacaggtaca cataagcagc
    14761 gtcatcaatg acgattttct gtgcctgctg gtagtcccgg gtacgctgcg tctggtcggt
    14821 ggtcgcaagc gcattgcgca gtaacttatc gacctcactg ttttcataga acgagcggtt
    14881 acccggcaga ccttttttgt ctgactcaaa ccagtaattc ataaacatat acgggtcggc
    14941 aaaatccgga ctccagttgc caatcgcaat gtcgtaatca cctttaccca ctctgtcgcg
    15001 catggtggcg ttcgccagct tttccagctt cacattgatg cccagcttgt tgagactgga
    15061 ttgtgtcgcc agagcaatag gctcccagtt cggatcctta tcggagtaga gaaacgtcag
    15121 gctggtgggt ttgctcgtca ctttatccca ttcggctttg gctttcgttt cgtcatggtt
    15181 gtattgcatt gccgtcgcat cgtagcccca catgccttcc ggaatcgggc cgcgcatctg
    15241 ttttccgtta ccactcagaa tgccgttaac catgccctga taatcggtag accaggaaat
    15301 ggcccgacgc agatccgcct gattaagagg cgctttgctg ttattcagat acaggtaggt
    15361 gacgcgcagt gacggatact ctgccacatt gactttgttt tcctgcttca gggcgttgag
    15421 ttgatccacc ggcagcgcat cggcaatgtc aatatcacca cgggagagct gcaggcgacg
    15481 ggaggcactt tcaccaataa tttttaccga tactcgctta aagttcggtt tattgccagg
    15541 gtaatgcgga tttggcacca gaactaattg ctgacctttt tgccagcttt tcagcataaa
    15601 tggtccggaa ccggcggtat tttgcgcgag gaagccgcgg gcatcatccg ctgcatgttc
    15661 ctttaacacc gccggattga taatggatgc accgtcattc gccagcgtgt agaggaacgg
    15721 tgcgaatggc tggctaaggg taaacttcac cgtatgttcg tcgggagcat caatctttaa
    15781 atctttggga aatgcttctg ctggcccctg gccgattttt agtagccgct caaaagagag
    15841 ttttactgct tcggcagtga ccggtgtgcc atcggcaaat ttagcattgt ccttcagggt
    15901 gaacgtccac tctttttgat cgtcagacgc tttccagcta cttgccagat cgccttcaac
    15961 gtcggttgag cctttatcac cgtccgtttt gtactgaacc agccgctgat aagacgggta
    16021 ggtcaccgtc cagtcgttat tatctattgt taccgccggg gcgagggttt gtggatcggc
    16081 ggccttacca atcaccagca tatctttcgg tacggcggcg tgcgcaaccg ggaaagttgt
    16141 ggcaaggacg agcgcgagca atgtaggacg aaacggtatc gatctcttca tgacagtttc
    16201 ctgattaact gacgtgctgt gtgccagggg atataaaaca ggtgaattga tcagcgagca
    16261 gtgggtaact cgctgcctgg ggtaattcga agtgccacca ttcgctggag ataccgacaa
    16321 aaccgccacc agtcataatc gcattcagca acagccgatt gcgctgagcg gcgggcggga
    16381 cggaagggtg ataggcatgg gagcgctcgt gcatttcatc gaaccctgcg cccatatcga
    16441 ggatgttccc gtgctcatca cgaagcgtca ggtcgatcgc cgtgccacgg ctgtgattag
    16501 aaccgaccgt cacatcaaca acatattgcg ggtctgggca ggcttgccac aacatcgcct
    16561 gtgcttgttg tgggcgatac gcatcgtaaa tcaccagttg taagcctgac agctgggcga
    16621 tactgatact tttcgccagc gcggtaatcg catccttgtg tagcagacaa cgcgcttgct
    16681 gataaatagc tttacctgtg atgttatcag cgcaggcgta tttcaattcg atctccagat
    16741 cagggaagat tacggctaaa tcaaccagtt cggtggtatc cgacataaac ggttcctgta
    16801 taagacaaaa attgctgcgc tttccgctta tgcagatctc atgccatgcc gggataagcg
    16861 ccagaatgct ggcttaaagt tatatttttt aacttttgat caacatttgt gcagcgtagt
    16921 gcagttttgg tgcaagaggg gaagttaagg aaggaatctc ccggaatcgt agatgaaatc
    16981 acagtattta agtgacggtg tcacgttaaa tgaaaacccg cgagtgcggg cgagaggaat
    17041 ttgtcagatt ttcagcggta acacgctgct catccagcct ggaatttctt cggcgggtag
    17101 ggggcgggaa aagaaatatc cctgaataac gcgacagtgg atcttgcgta gcatctcaaa
    17161 ttgctctttg gtttcgacgc cttccgccac gacggttaaa ttgaggcttt gcccaatgct
    17221 ggtaatggct tcaagtaagg caaggatgcg tttttcggtc agacaacgat cgacaaaact
    17281 tttgtcaatt ttgatttccg ttaccggaag actgactaag cgggataatc cggaaaagcc
    17341 cgtaccaaaa tcatctaccg ataagcccac gcccatatca cgcaggatct gaatgcgctt
    17401 aaagatttcg gtatcgtgtt ccatcatcat gctttccgtg atttctaccg tcagctggtg
    17461 gccgtcaata ccccaggcgt gcattgcatc agacacctga ttaggcagtt gattactgcg
    17521 aaagtgcagt gccgacaagt taacggaaag cgccgggata tgaatattct ggctacgcca
    17581 ttctgctaac tgacggcaag cttccgcgat gacccagcgc ccaatatttt cgatttcacc
    17641 aatctcttct gcgagaggaa taaaccgtga agggggcaca tgaccatgct ggggatcgtg
    17701 ccagcgcgca agggcttcga tgccgtacag ttcacccgtt tctgcgaaga tttgcggctg
    17761 gtaaacaagt ttcagttggt tattgctaat cgcttctttc agcgctgcgc ctaaaaccaa
    17821 acgctctttt accatttcgt tcatcgccgg gctgaagaac tgccagccgt taccgccatt
    17881 cttgcgaata taatctattg cattgtgagc agtggagagt aggtaatcgt ggtttttacc
    17941 cacgtcgtag ctgatgccaa tactcaaggt aagcgggaat ggtttatcgt caatcattat
    18001 cggcttgctg accacattcc gtagctcatc ggcgatctgg gtaatgttac tgacgtcgtt
    18061 ttccaggctc acgaggacaa actgcgtacc ttcgatacga cagagatact gatccggttt
    18121 gagttttcca cgaaagcgat tgaccacttc cagcaatgcc tgatcggccc acgcatagcc
    18181 aaggctatca atcacatcct gaatatggtc aacaccgatg agatacacca ccggagagac
    18241 ggctttgtcg accaggtcat cgaggtaatt gtgcaggtta ttgcgatttg gcagaccggt
    18301 catcggatca aattggatga gttgttcaat atgctggcgg cttttttcct gttccagcgc
    18361 cagcgcggcc atatgctgac tgatatctgc cacgcgttcg ataaaggcgc tggtttctgc
    18421 tcctgacgag gttttaattt gcaggatccc cgcaggcgcg ccatcacgct gacgaatggt
    18481 cgctgaccag ctttgcgcat tttgaacttc tgcaccgtgc gaagatgacg cccagtgtat
    18541 cggcatcccg ttgcgcagtg cgaacagcga aacatgcgat tcgttgagaa cagattcgat
    18601 gttacgacaa atgatttccc ccatttcatg aaatggcggg ctgctgcaca tggcggcgag
    18661 aatattgcct tcaagctggc ggatttgccg ttcttcggtg atatccgaga aagtcattac
    18721 caggttctgc agatgcgcga gcacgtcata aaccgggctg atagaggctt taatccagat
    18781 tttttcaccg gtgcgcgtca acagcagaaa ttcgtcctga tcgcgggcgg ttttccatag
    18841 caactgttgt aaacgaatgc ggttatcggc agggaattca ggaatgttca ggagtgtatc
    18901 gggctgcata ccgctggctt cgctaatgca gtaaccaaac atttcggtaa atgcgcgatt
    18961 gcactgcaca atatggcgtt ccggatcgag gacaatcacc ggtcggtcga gatggtcaac
    19021 ggcaataatc aattgtcggg tctgttcttt ttgcgccatt tctacgctgg catcccgtac
    19081 cagcgccagg taataaactt tcccctcggc gctcactttc gatagcgcaa aacgggtcca
    19141 gattttactg ccgtcttttt tctccagctg cagctcccga ctcatcccct cgacgcgcgc
    19201 tttaccacct tcacggttat gacgaatgta ttcaggatgc gcaggacgca aatcccgcgg
    19261 aatcagcata tcaatgttat tgccaatgac ttcttcacgt ttgtaccccc agagcttctc
    19321 tgcggcgggg ttgaaaaaca tcacttcatc attttcgtta attaacaccg cgcccatcat
    19381 attttgttca agggcgggga aaaaaatgcc atcggcggca ttttccgcat cggttagctt
    19441 catgattacc tctgcatcct ggcgcatcta aagactggct ttccagagtt caacacggtt
    19501 tctacctcgt cttttggcga tatacagagc ttcatcggct atttgaatga ggcgctcata
    19561 gtcaggatga ccattaaaca tggcggcacc gatggaaagt gagagggcaa tatcctcgcc
    19621 gtttgcggct ttcagtttgg ttttctccac ccgactgcga atacgttctg cggtacgtaa
    19681 cgtttcgttt tcagaagctt cagtcaaaac aatgataaat tcatcgcccc cgtagcgaaa
    19741 aacataatca ctactgcgga cgttgtcata aaaggcctga gagactttac gcagaatttc
    19801 atcgccagtg ttatggcccc acgtatcgtt gatctctttg aatttatcaa cgtcaataat
    19861 cagcactgac agcggtgtac cggtccggtt ggcatgggca atttcgcgtt tgaagatagt
    19921 cggcaggaag cggcggttaa gtaatttcgt cagtacatcc ataccgactt cgtggcgcga
    19981 tacttcttca aacaattcac gcagcaaggt aataatttgc gatacagtat ttcttatctg
    20041 taataaaaat ttcacccgca gacttctgtt attcaaattc ctggtgttac gcatggtttg
    20101 attgaatata ccgtcgaaat cctgaatcag acgggagata tgcctacttc ggcaatgcca
    20161 ctaaaataat gtcgaccttt atggttaaac cacaggccaa aatcagcctg gcttaaaggc
    20221 aaactactgc ctaaatcaga atccagcagg attttataga taatatctat ttcccatgaa
    20281 agtattgagg ctatttgccg ttctttttct tcttcggcgt tttctagtaa cgagaagata
    20341 cgatagtttt catcttcctt tgaggcacta ctgtcactaa aggtaaacgc gcgggtcatc
    20401 acttccatag cgatatcaat actgttaatc gagaaatggt agacctgaag tttttctgcg
    20461 gcagaataat ccgaagagaa gattaccgga tagaggattt ttttcagcac ccgaaacccc
    20521 atctcgacaa tttctaccgg aattcctatg cgggcatgca cttccgcgac ggtatgctgg
    20581 atttgtatta gcctttcgac atcgtcaacc tgggcagaaa gcacgttaat aatccagcgt
    20641 tccatcgcac ttttcaactg ccgctcaact tgttcattac tcaagaattc ttcggcatgc
    20701 gggtcgatgc ggacaattcg ataaaactcg atactcagat aatgagcatg cgcaacggca
    20761 atttccgcgg ctttagcacg aatgagtgga tctgcccgtt cgacaagtcc ggtccactca
    20821 tctttcattc ttttaaaata catctccata attcacaccc ttataaggct gggaaatcag
    20881 acggaatcaa aacgcaacgt gcgagatcga ctaactgcac catattctcc tgaaatatga
    20941 agatatactg aaaagaaata agcgacttag gacagtttca atctacgcta ctgttcttca
    21001 gaagagtata gcccatcgta attatttttc ggtgacagcg aatatcgtat ggtttttcat
    21061 attcatacat ttttattagg gatttatagc tgtttaacta agtgtggtta attgcgtggt
    21121 taatttgact tatgtaagca tgattactag tgggatattt cacaaggtgt aacaagccgg
    21181 tgggtaaagc accggcttgt tacaaagtaa gcatgggagt ttaactgccc caacgacttt
    21241 gcagatagct gaccgcttgt tgagtctgcg gtttattcag atagtcctca cggaacaaga
    21301 tggtgccgct aatttctggt accgcatcgt taagatcgag ctgctttttc agttccggta
    21361 cgccgccgtt aatcatccag tctggctcta tctttgaagg ttcacccact ttatagaagg
    21421 cgataccgat atacaggcgg gtcctggtcg gtttaacgac atccgcccac cattttgcca
    21481 acacgtcata acgcgcggca ctccgtgaga acggccagta aatttgggga gcaatgtaat
    21541 ccagcaatcc ttgttccacc catcgacggg tgtcagcgta ggattcgtca taggctgccg
    21601 cgcctcgggt atcggaaccg agcggatcgt gtgatcggtt acgccacacg cctgccgggc
    21661 taacgccaaa ttcgactccc ggcttaatgc ttttaatggt gtgcgatact tttgtaatta
    21721 actgctgagt attgttgcgc cgccagtctg cttttgacgc aaatgcgcct ccgtatttac
    21781 ggtacgtttc gttatcattt agccgtgaac ccggtgactc cgtatagaaa tagtcgtcaa
    21841 actgcacgcc atctaccgga tagcgggaaa ccacttcggc gactattgat gtgatccagt
    21901 cctgaacctc agggatgccc gggtcgagga caaagcgatc gccagacgtt ctgatccagt
    21961 cacggtgttg cacatagacg ctcgccggtt gttgagacag agtgctattc agttccctga
    22021 tagtaccggg cttcgtatta accgatacgc gataggggtt aaaccaggcg tgtactttca
    22081 tcccacgctt gtgggcttcg tcgagcataa attgcagcgg atcgtaaccc ggattttcac
    22141 caatcttacc ggtcataaga tcggaccacg gcaaaatttt tgatggccac agggcggtac
    22201 cgtccggctt gacctggaaa aagaccgtgt ttataccgag acgttgcaga tgatccagtt
    22261 tgtcgatcat cgcctgttgt tgtacacggg cccggctggt ggggttacta atgttaaccg
    22321 aggaaaccgg tggccagtcg aggcgagaca ccgtggccag ccagatgcca cgcatcggtt
    22381 gtgacgattg ttgcgtcgtg gctggtggct ttgaacctgc tggtggtgtc accatggact
    22441 ctggaggcgt gcttttacaa ctacacagta aaagtgccag tgcaaccagt atcgctggtc
    22501 ttctaatcgt taatttcttg tttcgggagc agatatccat atgtgctggt ttccggtaaa
    22561 cagatgtgcg ctcattctcg tacttatccc ggttaagtca atacgacagc aagcacgaaa
    22621 aagggagcga tgaattatcg ctcccttgtc ttataaccat tcagacatgg ttagtgtttc
    22681 ttgtcattca tcacaatata gtgtggtgaa cgtgcacgcg ggtgcaggaa gaagtgacct
    22741 tttggtgcgt tctgactgtt gattggctcc agagtgacgc cggtattcgc tttgccttta
    22801 cgatcatgaa cagcatagag aataaagggc agggcaagta ccaccaggaa actaacgacc
    22861 agtaattcaa catacatatc ggtagagtca ccctggatgt tatccggcgg caggaaggaa
    22921 acaataaacg ccataattga agtcagcaga ccgacaattg ccacgaccag ttttacccct
    22981 ttaccaccag ggatattaaa tgtgcgtttt aagtcaggat gtttaagaac taacacaatg
    23041 tagccaataa acagcatgaa ataagcacac agataaatca ccaccgtcag cgccagtgcg
    23101 atcaggaagg acatgttgtt accgccaccg gtattggtga ggatgatcaa cgcgatagaa
    23161 gtaatcacca gctgcgaaat gaccagcgtt accggtacgc catttttgtt cattttagcg
    23221 aatgccgctg gcagcaggtt tttctgcgct gtgacataca tcccgcgaga aggaccaaca
    23281 atccaggagg cgatttccgc cagaacaccc agcaacagca gtgcggagat cacgcgaacc
    23341 gtccactcaa tttccggtgc cacatgggac atcagaacgg taaaggtttg cattacccct
    23401 gcggagaggt tgatttcatt acccggaatg accatcgcaa tagataaacc gccaacagag
    23461 cttaagcaga ttgccgccac catcagcagt aacatagcca gtggatagtc gcgcccgggg
    23521 ttgctcattt cattgacgtg agttgcggaa gcttctacgc ccatataact caaaatgaag
    23581 gcaacaaata caaccagtgt gcccacttta gagaagtcag ggaagaaggt cttcgaatcc
    23641 atttcgatag caacgggggc accggagtgc agataaatag ccgctaatgc gatcaaaata
    23701 aatgcaggta acaggatacc ggcgaagaag ccaactttag caattcgcgc cgtgtatttc
    23761 gtgccaccaa actgcgttaa tgccagcgcc caaagaatga tgagtgctgc aatagttttg
    23821 gtaatggggt cttcattcag cgctggccat ttcaggatgt aggagagtgc ccctaacacg
    23881 aaatagagca tcggaataaa accaatggcg atttgcagat agccaaatga gatcgctgca
    23941 aatccccatc tcggccccag agtatttgat acccaggcga agacaccacc ttcttcccag
    24001 ccgtcgacgg tggccatttc cgcagcacaa agtcccacgg gaataaacca taaaatcccg
    24061 cctaatagca ggaagaagac taatgaaaag cccgatgttg cgaaggtagg gtattcataa
    24121 acagccatta ccatcgatgc cgttatggca aagaatccga gtaatgtgag ctgcttagct
    24181 ttacctgtct gtactgatgt agccatatta tccccctaaa acggtattcc tgtcggaacc
    24241 gcacctgttt tgttctgaag cgtattcaga acaatatttt ccgttgctaa tgccagtgaa
    24301 cagactttgg aaattgtccc gaaacgggtt cgtttcggga caccgttacc gttaaacgtt
    24361 atcaggtatg tttaaagctg ttctgctggg caataccctg cagtttcggg tgatcgctga
    24421 gatatttcag ggaggctttg tagtcttcca gcaacagttc agcaaagtcc atttcgaagc
    24481 cgcgacgaca cataat
//
LOCUS       DAJTOR010000047        24154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTOR010000047 DAJTOR010000000
VERSION     DAJTOR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.047"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..2599)
                     /gene="valS"
                     /locus_tag="OO249_001691"
     CDS             complement(<1..2599)
                     /gene="valS"
                     /locus_tag="OO249_001691"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5636030.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVSL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSV"
     gene            complement(2599..3042)
                     /gene="holC"
                     /locus_tag="OO249_001692"
     CDS             complement(2599..3042)
                     /gene="holC"
                     /locus_tag="OO249_001692"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5636031.1"
                     /translation="MKNATFYLLDNDTTVDGLSTVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3202..4713)
                     /gene="pepA"
                     /locus_tag="OO249_001693"
     CDS             complement(3202..4713)
                     /gene="pepA"
                     /locus_tag="OO249_001693"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5636032.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            4980..6080
                     /gene="lptF"
                     /locus_tag="OO249_001694"
     CDS             4980..6080
                     /gene="lptF"
                     /locus_tag="OO249_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5636033.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6080..7162
                     /gene="lptG"
                     /locus_tag="OO249_001695"
     CDS             6080..7162
                     /gene="lptG"
                     /locus_tag="OO249_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5636034.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7323..8825)
                     /locus_tag="OO249_001696"
     CDS             complement(7323..8825)
                     /locus_tag="OO249_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5636035.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(8955..9974)
                     /gene="ahr"
                     /locus_tag="OO249_001697"
     CDS             complement(8955..9974)
                     /gene="ahr"
                     /locus_tag="OO249_001697"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5636036.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            10170..10254
                     /locus_tag="OO249_001698"
     tRNA            10170..10254
                     /locus_tag="OO249_001698"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10204..10206,aa:Leu,seq:caa)
     gene            10418..11683
                     /locus_tag="OO249_001699"
     CDS             10418..11683
                     /locus_tag="OO249_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5636037.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQGIDPKAEQKEAQAENSGAYSFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYR
                     GMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MVQWWADYLQANKGKTITPYDFAKINKSR"
     gene            complement(11738..12748)
                     /locus_tag="OO249_001700"
     CDS             complement(11738..12748)
                     /locus_tag="OO249_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636038.1"
                     /translation="MIYITSESKETLMIKYEGKMKKNIRVIPKNIHSKLRRLGNTVVA
                     GTSIAFTENQLKSGALEHLGIYFDNGVNAYETSVIPDPLQGKYSLKNVFGEEIVRKDL
                     PKETHYTEIESPNWGDSSNGTHTVRLPYEKYPRDIIPPTLIAIEINHKQPSDSHFIFN
                     FRVTRILDKQSNKFDDELLFDLNLLQENLGKCGIEDADKPISTYADTLIVSWEIFPPG
                     SKEETLARIFRGKNITSDKKNVAENRYDFFMSLEPKKIVTGNSTFSNYIGAMLEDDLV
                     VFENIEYGNAIYILYDNWDDISKLSRIDLLSGRAGSNFDRIIHSGNWKDEVRKKVAAG
                     RL"
     gene            complement(12699..13235)
                     /locus_tag="OO249_001701"
     CDS             complement(12699..13235)
                     /locus_tag="OO249_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636039.1"
                     /translation="MLKAIAGIAKYTWIYMAIFIISLMIISSSDINYVYNDFANYCTL
                     LVSVSGMVFTIMGIWLAFLYPNALQRIVNPKTIETVDFTEQLEDTRRLEAIVGSVLKS
                     SFVMITLLVVYLVKLVFFKSHLYLESQDVIKNTSLALIIAISYMQIEAVISVILANVM
                     FINDLHNKREQRDIDDQV"
     gene            complement(13236..14315)
                     /locus_tag="OO249_001702"
     CDS             complement(13236..14315)
                     /locus_tag="OO249_001702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636040.1"
                     /translation="MNVGHLNFFKVNKCGLYKVNDDNTYGLELSETFDLIQDWVGTKS
                     LALTIPWDPKEKPNSSKCYCKDIYKDENTGDFLVMLWKSDTDSTGSLLGASEDGEIGS
                     SSVVKYTNSYRGKKVIWGRPCFYWVIPELETIVSIKFDHSVCDSELFQDYVHSSITNR
                     VKHSKRVKNKTEKGYIRLSNTDDEDLYKYMYRFDMKLRSLETTHTELGKLIPKITHIV
                     RRETIIINPNDARADWLKTFSTLVPFVSGKKNAKTRQIEIKAEAKPSLNEVQEIIEKY
                     SAEDREKRLWDNVGFATDKGITWVDKYRMRDILNVPSDDYSTYSAAYIYEQISHKRKE
                     FISPILKELKALQSQPRIKKASGED"
     gene            complement(14713..15285)
                     /locus_tag="OO249_001703"
     CDS             complement(14713..15285)
                     /locus_tag="OO249_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HCR5636041.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAT
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(15359..15859)
                     /locus_tag="OO249_001704"
     CDS             complement(15359..15859)
                     /locus_tag="OO249_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5636042.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(15856..16590)
                     /locus_tag="OO249_001705"
     CDS             complement(15856..16590)
                     /locus_tag="OO249_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HCR5636043.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            17142..17408
                     /locus_tag="OO249_001706"
     CDS             17142..17408
                     /locus_tag="OO249_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5636044.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            17405..18004
                     /locus_tag="OO249_001707"
     CDS             17405..18004
                     /locus_tag="OO249_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5636045.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            17997..18284
                     /locus_tag="OO249_001708"
     CDS             17997..18284
                     /locus_tag="OO249_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HCR5636046.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            18277..18732
                     /locus_tag="OO249_001709"
     CDS             18277..18732
                     /locus_tag="OO249_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636047.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYQDIYPQDTVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            18868..19188
                     /locus_tag="OO249_001710"
     CDS             18868..19188
                     /locus_tag="OO249_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HCR5636048.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            19203..21536
                     /locus_tag="OO249_001711"
     CDS             19203..21536
                     /locus_tag="OO249_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5636049.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDVAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEVTRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPLDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            21702..21896
                     /locus_tag="OO249_001712"
     CDS             21702..21896
                     /locus_tag="OO249_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5636050.1"
                     /translation="MWIKDALVWIRATDPNLLIKNYLTFRFIDGLKIDWSEKQWGHKR
                     GHISLLCFIICFLSLTYFDI"
     gene            22114..23382
                     /locus_tag="OO249_001713"
     CDS             22114..23382
                     /locus_tag="OO249_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5636051.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     AKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANREKGITPFDYAKINRGNGE"
     gene            23773..>24154
                     /locus_tag="OO249_001714"
     CDS             23773..>24154
                     /locus_tag="OO249_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636052.1"
                     /translation="MNFKKKLEEHFKQFEASPVLFVGSGVSRRYLGVPCWQDLLKHFA
                     EAIGENHIKLKTKSNGDLPEYAQLLVSAYAEKWWDTEEGQLALSEKEQEKTFINEQSP
                     LKLSISKYIENAHKNIIDNDELKHE"
BASE COUNT         5751 a         6249 c         6013 g         6141 t
ORIGIN      
        1 taacggaaac cggacctttg tcatcggcag gcagcacggt gatgctttcc agacgcgcca
       61 gggtttgcag gaagccacgg ttttcattta cgcgacgttc tgcatccgcg ctgcaaccac
      121 gcagcagcag ctccagcggt ttgcccggcg cgatgttcat ttctgcacgg atgttacgta
      181 ccgcaacgat cgcctgtttc agccactcgg tgtcagccag tgcggcttca tcaacctgag
      241 atgcatcgta ctgcgggaac ggctgcagca tgatggtgtc ggcagtgata ccgcaaagta
      301 ctttcacacg ctgccagatg gtttcggtga tgaacggaat gatcggatgc gcgaggcgca
      361 gcagaccttc cagtacagtc accagcgtat ggcgagtacc gcgcagttcg gcttcggttc
      421 caccgttcat taccggctta gtcagctcca gataccagtc gcagaactgg ttccaggtga
      481 actcatacag aatgcctgca gcgatatcga agcggaagct gtccagcgct tcgcggtacg
      541 ctttgatggt ctggttgaac tctgccagaa tccagcggtc cgccagcgac agcgtcattt
      601 caccaccgtt gaagccgcaa tcctgacctt ccgtgttcat cagcacaaag cggctggcgt
      661 tccacagctt gttacagaag ttacggtagc cttccaggcg tttcatatcc cagttgatgt
      721 cacgaccggt agaagccagc gccgccaggg tgaagcgcag ggcatcggtg ccgtgcggct
      781 caatgccgtt cgggaactgt ttctcggtgc gcttacggat tttgtccgcc agctgcggct
      841 gcatcatatt gccggtacgt ttctccagca gttccggcag cgaaataccg tcaaccatat
      901 ccagcgggtc aataacgtta cccttggatt tggacatctt ctggccttcg tcgtcacgaa
      961 tcaggccggt catgtaaacg gtgtggaacg gcacctgcgg tttgccattt tcatctttga
     1021 tgaagtgcat ggtcatcatg atcatgcggg caatccagaa gaaaatgatg tcgaaaccag
     1081 ataccatcac gctggttggg tggaactggc gcagtgcgtc ggtgttttcc ggccagccta
     1141 gggtagagaa ggtccacagc gcagaagaga accaggtgtc gagaacgtct tcgtcctgac
     1201 gcagggcaac gtcggcaccg aggttatttt ccttgcgcac ttcttcttcg ttgcggccaa
     1261 cataaacgtt acctgcttcg tcgtaccatg cagggatacg gtgaccccac cacagctgac
     1321 gagagataca ccagtcctga atatcgcgca tccaggagaa gtacatgttt tcgtactgct
     1381 tcggtacgaa ctgaatgtcg ccgttctcaa ccgcctcaac cgccggtttc gccagtacat
     1441 cggcacgcac gtaccactgg tcggtgagca tcggttcgat aactacgccg ccacggtcgc
     1501 cgtaaggcac ggtcaggtcg tgcggtttaa tttcttccag caggccgagc gcgtcaaccg
     1561 cagcaacgac tgctttacgt gcagcaaaac gctccagttt ctggaactct gccgggattt
     1621 cgctggaata aacgtcagat tcgttacctt tggtatcgaa cacctgggcg ctttcacgga
     1681 tatcgccgtc aaaggtcagg atgttgatca tcggcagcgc gtgacgttta cccacttcat
     1741 agtcgttaaa gtcgtgcgcc ggagtgattt tcacgcagcc ggtgcctttt tccatgtcgg
     1801 cgtgttcgtc gccaacgatc ggaatacgac ggttaaccag cggtagaatg acatatttgc
     1861 caatcaggtc tttgtaacgc ggatcttccg ggttaacggc cacgccggta tcgcccaaca
     1921 gggtttccgg acgggtagtc gcaaccacca gataatcttt accgtctgcg gttttcgcgc
     1981 cgtcagccag cggatagcgg atgtgccaca tcgaaccttt cgattcgcgg ttttccactt
     2041 ccaggtcaga gatagcggtg cgcagtttcg gatcccagtt taccaggcgt ttgccacggt
     2101 aaatcaggtc ttctttatac agactaacga aaacttcttt caccgcattg gacaggcctt
     2161 cgtccatcgt gaagcgttca cgctcccagt cgacggagtt gccgagacgg cgcatctgac
     2221 gggtaatcgt gccgccggat tccgctttcc attcccaaat tttgtcgatg aaagcttcgc
     2281 ggccgtagtc gtgacgggtt ttaccttctt ctgcggcaat cttgcgctca acgaccatct
     2341 gggtagcgat cccggcgtgg tcagtaccga cctgccacag ggtgtttttg ccctgcatgc
     2401 gctgatagcg gatcatggta tccatgatgg tttgctggaa ggcgtgaccc atatgcaaac
     2461 tgccggtgac gttcggcggc gggatcatga tgcagaaact ttcctggctt tcatcgccat
     2521 taggcttaaa gtagccctgc ttttcccagt gctcgtaaag cggctgttcg atatcttgtg
     2581 ggttatatgt cttttccatt atttccaggt tgccgtattc aggttgaaac cagccacgcg
     2641 gtaggcttta tagcgttcgc gcgccagttg tttcagagaa tcttcataag gaacgaagtc
     2701 taccacttct gtgaaagcgg tggcaaaatc tgcaaagctt gttcgcagac taatcaatat
     2761 atcgcgcggg ctgctgctgc gtttttgcgg ccaggcgatc tccaccggcg caccgccgcg
     2821 cggtccttct cccgctaaat tatgcggaac aaagctttct gccggacgcg cccacagggc
     2881 ttcatccagc cggtaagcct gcttttcatc ttcacaggcg atgagcacgc gcttaccgct
     2941 gcgccaacgt tctgcggcaa tttcacacac cagttgctca acggtgctta agccatcgac
     3001 ggtggtgtca ttgtccagaa ggtagaacgt cgcgtttttc atatatgggg cttcttgtgg
     3061 tggatttaaa tacgcatcag gtatacaccg cagcgcctga tgcgacgctg ttgtgtctta
     3121 tcaggcctac gagttcagtg ctgtgtaggt cggataaggc gttcacgccg catccggcaa
     3181 taacagcctt gcctgacgca attactcttc gccgttaaac ccagcgcggt ttaacaggaa
     3241 ctgtgccagc aacgccaccg gacgaccggt ggcgcctttt gctttaccag aacgccaggc
     3301 ggttccggcg atatccaggt gcgcccagtt gtacttacgg gtaaagcgcg acaggaagca
     3361 acctgcggta atcgccccac caggacgacc gccaatgttc gccatatcgg caaaattgga
     3421 ctccagctgt tcctgatact cgtcacccag cggtaagcgc catgcgcggt caccggattg
     3481 ttcagacgcg gcaatcagtt catgggccag cggattatgg ttcgccatca gaccggtaat
     3541 gtgatgaccc agcgcgatca cgcaggcacc ggtcagcgtc gccacgtcaa tcaccgcttc
     3601 cggctcaaaa cgctcaacgt aagttaacac gtcgcacagt accaggcggc cttcagcgtc
     3661 ggtattcagc acttcaacag tttgaccgga catggtggtt aacacatcgc ccggacgata
     3721 ggctcgtccg ccaggcatgt tttcgcagcc tgccaacacg ccgataacgt taatcggcag
     3781 ttgtagctcc gcgaccatcc gcatcacgcc gtaaaccgct gccgcaccgc acatatcgta
     3841 cttcatctca tccatgcctt ctgaaggctt gatcgagata ccgccggagt cgaaggttaa
     3901 acctttaccc accagcacga ttgggcgtgc atcttccgac gcgttgcctt tgtactcaat
     3961 caccgacatc agcgattcgt tttgcgaacc ctgaccgacc gccagatagg aatgcatccc
     4021 cagctctttc atctgctgtt cgccgataac gcgggtgatg acattcttgc tgtagctgtc
     4081 agccagctgg cgcgcttgtg aagcgaggta agcggcgtta cagatattcg gcggcatatt
     4141 gccgagatct tttgctgctt taatcccggc ggcaatcgcc agaccgtgca ggatcgcgcg
     4201 ctcaccgctg gtcagttcac ggcgggtcgg cacgttgaac accattttac gcagcggacg
     4261 acgcggttcg ctcttgttcg ttttcagctg atcgaaactg tagagcgtct cttttgccgt
     4321 ctcgacagcc tgacgcactt tccagtagtt gttacggcct ttaacgtgca gttcagtcag
     4381 aaagcagacc gcttccattg agccagtatc attcagcgta ttaatggttt tctgaataac
     4441 ctgcttgtac tgacgctcat ccagctcacg ttctttgccg caaccaataa ggagaattcg
     4501 ctcggaaagt acattcggaa catggtgcag caacaatgtc tgccccggtt ttccttccag
     4561 ttcgccccga cgtagcaggg cgctgatgta cccatcgctg attttatcga gctgttctgc
     4621 aatcggagaa aggcgacgtg gttcgaagac gcccacgacg atgcaggcac tccgctgttt
     4681 ctccgggcta ccgcttttta cactaaactc catgcactac gctcctgaat cttaaagaca
     4741 acggcggtgg ctacagatag aattgcaagc tttcgtaact catgtccgct gttgcgatga
     4801 cttcgtgtta atcttaacgt tattacggca ttggcacgtc agaacaaaat ctgagaggtg
     4861 aatccgttga gtataattat cttagcgacg atttcgacga ctcaagagaa taaatgacgt
     4921 ttaagccatg aaacaagcta aaatcctgca aaagacgagt ttttacgggc gtatttaaag
     4981 tgataatcat aagatatctg gtgcgggaga cgctcaaaag ccagctggcg atactcttca
     5041 tcttgctttt gatcttcttc tgtcaaaagt tagtgaggat cctcggcgca gcggttgacg
     5101 gcgatattcc ggcgaatctg gtgctctccc ttctcgggtt gggcgtgccg gaaatggcgc
     5161 agcttatcct gccattaagc ctgttcctcg ggctgctgat gacgctgggc aaactgtata
     5221 ccgaaagtga aattacggta atgcatgcct gcggcctgag caaagcggtt ctggtgaaag
     5281 cggcaatgat ccttgcggta ttcactgcaa tcgtcgcggc ggttaacgtg atgtgggcgg
     5341 gaccgtggtc atcgcgtcat caggatgaag tgttagcaga agcgaaagcg aaccctggca
     5401 tggcggcgct ggcgcaaggg caattccagc aagcgactaa tggcagctcg gtgctgttca
     5461 tcgaaagcgt tgacggcagc gatttcaaag atgtgttcct cgcgcaaatt cgaccaaaag
     5521 gtaatgcacg tccttctgtg gtggtggccg attccggaca tttaacccag ctgcgcgacg
     5581 gctcccaggt cgtcactctc aaccagggaa cgcgcttcga aggcactgca ctgttacgtg
     5641 atttccgcat tacggacttc caggattatc aggcgatcat tggtcaccag gcggtagcgc
     5701 tcgacccgaa cgataccgac cagatggaca tgcgtacatt gtggaacact gacaccgatc
     5761 gtgcccgcgc agaactgaac tggcgtatca cgttggtatt caccgtgttt atgatggcac
     5821 ttatggtcgt accgctgagc gtggttaacc cgcgtcaggg acgcgtacta tcgatgctgc
     5881 cagccatgct gctgtatcta cttttcttcc tgatccagac ctccctgaaa tcgaacggcg
     5941 gtaaaggtaa gctggacccg acgctgtgga tgtggaccgt taacctgatt tatctggctt
     6001 tggcgattgt tctcaacctt tgggacaccg tgccggtccg ccgcctgcgc gccagttttt
     6061 cgcgtaaagg agcggtgtga tgcaaccttt tggcgtactt gaccgctata tcggtaaaac
     6121 tattttcacc accatcatga tgacgctgtt catgctggtg tcgctgtcgg gcattatcaa
     6181 atttgtcgat cagctgaaaa aagccgggca ggggagttac gacgcgttag gcgcaggaat
     6241 gtataccttg ctgagcgtgc cgaaagatgt gcaaacattc ttcccgatgg cggctctgct
     6301 tggggcgttg cttggtcttg ggatgctggc gcagcgcagc gaactggtgg tgatgcaggc
     6361 ttctggtttc acccgtatgc aggttgcgct gtcggtgatg aaaaccgcca ttccgctggt
     6421 cttgctgacg atggcgattg gtgaatgggt cgcgccgcag ggcgagcaga tggcgcgtaa
     6481 ctaccgtgcg caggcgatgt acggcggctc gttgctctct acccagcaag gcttatgggc
     6541 gaaagatggc aacaacttcg tttacattga gcgggttaaa ggtgacgaag agttaggtgg
     6601 catcagtatt tatgccttta acgagaatcg tcgtctgcaa tccgtacgct atgccgctac
     6661 tgcgaagttt gacccggaac ataaagtctg gcgtctgtcg caggttgatg aatctgatct
     6721 gaccaatccg aaacagatca ccggttcgca gacggtgagc ggcacctgga aaaccaacct
     6781 cacgccggac aaactgggcg tggtggcgct ggacccggat gcactctcaa tcagcggttt
     6841 gcacaactac gtgaagtatc tgaagtcgag cggtcaggat gccggacgtt atcagctcaa
     6901 tatgtggagc aaaatcttcc agccgctatc cgtggcggtg atgatgctga tggcgctgtc
     6961 gttcatcttt ggcccactgc gtagcgtacc gatgggcgtg cgtgtggtca ccggtatcag
     7021 cttcggtttt gtcttctacg tactggacca gatcttcggc ccgctgacgt tggtttatgg
     7081 catcccgccg atcatcggcg cactgttgcc aagcgccagc ttcttcttaa tcagcctgtg
     7141 gctgttaatg agaaaatcgt aaccgaatga acgaataaag ggagcgaaag ctccctttat
     7201 tattgttagc aaagtgtgct tcgttcatgc ctgaaaaatc atgaaaatcc aatatattgc
     7261 agtggtgtag gcatgataag acgcgccagc gtcgcatcag gcaaagacaa atctcgcctg
     7321 acttaccttc ttcttccccc cagcaaactc cccaacatcc cacgtacaat ctgattcgtc
     7381 acctggcggg cggcgctttt cgccattgtt tgcaccacac catctttctt cccgccgcgt
     7441 ggtccggtag tgccaaacaa aatatccttc aatccaccaa gaataccgtc atccaccgcc
     7501 accgctttcc ctttcgcggg aggattattt tgctgcttgg tactggcctg aaagcctttt
     7561 tgcaacatct cataggcgga ttcccggtcc acgtcatcct cgtacttgcc atacaccggg
     7621 gagtggttaa tcaagccatt gcgctcatct tccgtcaccg gtcccatccg cgaacaaggc
     7681 gcaatcacca tcgcccgttc caccacagaa ggacttcctt ttgcatcgag aaacgagatt
     7741 aacgcctcgc cggtccccag ttcctggatt gccttttcgg tatcaaacgc cggattggcc
     7801 cgcatggttt gcgccgcagc ttttaccgct ttctgatctt tgggcgtaaa agcccgcaaa
     7861 gcgtgttgaa cgcgattacc gagctgcccg agcacattat ccggaatatc agacgggttt
     7921 tgcgaaacga accagacgcc cacgcctttt gagcgaataa gccttattac ctgctcaatc
     7981 ttatccagca gtacctgcgg tgcatcgtta aacagcagat gtgcctcgtc gaagaaaaac
     8041 accagttttg gtttctccag atcgcccgct tccggcaatt gttcatacaa ctctgaaagc
     8101 atccacagca ggctggcggc gtacagtttc ggcatctgat aaagcttctc ggcgctgagg
     8161 atattgataa cgcctttacc gttggtatcg gtgcgcatcc agtctttgat atccagcatc
     8221 ggctcgccaa agaaatgcgc cgcaccttgc ttttccagcg acagtaaacc gcgctggatg
     8281 gcaccaaccg atgcgctact gatattaccg tactgattct ggaaagattt ggcgttatcg
     8341 ccgatgtact gggtaatcgc ccgcagatct ttaaagtcga gcagcaacaa tccctgatcg
     8401 tcagcaatac ggaagatgat attcagcacg ccagattgca catcgttgag attcaacagc
     8461 cgcgccagca acagcggccc caggtctgaa accgtcgccc gcaccggatg gcctttctcg
     8521 ccaaagatat cccacaccac caccggatta gcatgcggtt gccagtcatt gacgccgata
     8581 tttttaagcc ttgtgagcag tttttccgac gccgttcctg cctgcgcgat accggtcaga
     8641 tcgcctttca catcagccat aaacaccggc acgccgattt ccgacaatga ctctgccagt
     8701 ttttgcagcg taacggtttt acccgtcccc gttgcgccag taatcagccc gtgacggtta
     8761 gccattcccg gcagtaaaaa aagttctgtg tccggcgtgc gggcaattaa taggggttcg
     8821 ctcatgagat ttcctccagt ttcctgcctg gagtataggc aacggctgcg cactcttcac
     8881 cgctaaattc agcagattgc gagacgcctt ccaaagagat ttaagtgccg ggcgacaggc
     8941 acccggcaaa gggatcagaa gtcggctttc agaaccacgc ggtaacgcgc cttaccgtcg
     9001 cgcacatgct tgatggcatc gttaattttc gacatcggga acagttcggt ggtcggcgca
     9061 accttgctac gggcggcaaa gcgcatcagc tttcgcagct cataaggcgt gccggtagca
     9121 gaaccagaga tgctgcggtc gcccgcaatt aatgtaaagg ccggaacaga cagcggcgtg
     9181 agaaccgcac cgaccgtatg gaaattaccg ccataggtca gcgcctcaaa ataaggctgc
     9241 cagtcgaggc tgacgttcac agtattgata atgagatcaa actgccccga cagggctttc
     9301 agtgcctgcg gatcgcggct attcaccact ttatcggcac ccatcgccag tacttcctgc
     9361 tctttcgccg gattagaact aaaggccgtc acctcgcatc ccattgcgtg cagaagtttt
     9421 atagcgatat gccccagccc gccaatacca attaccccaa cgcggctggt agcagtgata
     9481 tggtgcatca acagtggttt aaagaccgtg ataccgccgc acaacagcgg cccggcggac
     9541 tcgatatcaa tattttcagg cagtggaatc acccattgcc agtccgcacg caacttctcg
     9601 gcaaagccac cgcgattcat aatcgtcggc accgcacctt gctcgcagtt gatctgatta
     9661 ccgctaatac aggcgtcgca gtgaccacag ctacgcgccg tccagccaat cccgacacgc
     9721 tgaccgacct gcaaaccttt atcctgcgcg gcgctcccga gtgccaccac gcgcccaatc
     9781 acctcatgcc cggcaaccag cggatattgt gaaaatcccc attcattgtc gatcatcgat
     9841 agatcggaat ggcagatccc gcaataatcc acctgcactt caacatcttg tggcctcagc
     9901 tcaccgggat cgtactcata aacttccagt tcgccgcccg cttcttttgc ggcatagctt
     9961 tttatcatcg acattttttg gtccttctct ggtgttgttt gggaagtgta gagcatggca
    10021 gggcggggtg cctggagtgt gacaaaggtt acacatcgct gtgtgcaatg ctgaaaattt
    10081 cagcacttag cgaggtgcga gcaagctggc gcttgcatgg tggcgtgcga caggtataat
    10141 ccacaacgtt ttccgcatac ctcttcagtg ccgaagtggc gaaatcggta gacgcagttg
    10201 attcaaaatc aaccgtagaa atacgtgccg gttcgagtcc ggccttcggc accattctta
    10261 cttccaagac catccgagaa agtccaaata tccctttaaa atcaatgctt gcagcgattt
    10321 tttcgtcctg cgtcttccga ggttgtccgt tgaaatccgg atatcattgg gggcataatt
    10381 gggggcatct taacttcgat tagaaatgtg cccccaaatg aagctcaacg ccagacaggt
    10441 cgagaccgca aagccaaaag acaaaaccta caaaatggcc gatggtggtg gtttgtatct
    10501 tgaggtttcg gccaaaggtt ccaaatactg gcgcatgaaa tacagacgtc cctctgacaa
    10561 aaaagaggat cgtctcgcgt ttggtgtttg gcctactgtg acgcttgctc aggcaagagc
    10621 aaagcgcgat caagctaaaa agctgttagc gcagggcatt gatccaaaag ccgaacagaa
    10681 agaagctcag gccgagaatt cgggggcata ttctttcgaa actatcgctc gtgaatggca
    10741 tgctagtaat aagcgctgga gtgaagatca ccgatcacgc gttctacgtt atcttgagct
    10801 ttatatcttt cctcatattg gttcatctga catccgtcag ctcaaaacta gccacctatt
    10861 agccccgatt aaaaaagttg atgccagtgg taagcatgac gttgcacagc gccttcagca
    10921 gcgtgtcacg gctattatgc gttatgcagt acagaacgat tacatcgact caaatccagc
    10981 cagtgatatg gccggtgcgc tatcgacaac caaagcgcga cattaccccg ctttgccctc
    11041 cagccggttc cctgagtttc ttgctcgtct tgctgcatat cgtggccgtg taatgacgcg
    11101 gatcgcggtt gagctttcct tactaacttt tgtgcgttcc agtgagttac gtttcgcgcg
    11161 ttgggatgag ttcgacttcg ataaatctct ctggcgagta cctgcaaaac gagaagaaat
    11221 taaaggtgtg cgttactcgt accgtggcat gaagatgaaa gaggagcata tcgttcagct
    11281 tagtcggcag gcgatgattt tgctaaacca gttaaagcag attagtggag ataaagagct
    11341 gctttttccg ggtgatcatg acgcaactaa ggttatgagc gaaaacacgg taaatagcgc
    11401 attgcgtgcg atgggctatg acacgaaaac cgaagtttgc gggcacgggt ttagaacgat
    11461 ggcccgtggt gcgttgggcg agtccggatt atggagtgaa gatgctatag aacgacagtt
    11521 gagccattca gagcgtaata atgtacgtgc tgcttatatt cacacctctg aacatttgga
    11581 tgaacgtcga ttgatggttc aatggtgggc tgattatctc caagcaaata agggcaaaac
    11641 aataacacct tatgattttg ctaaaataaa caaaagtcga tagtgaaaat aaacgcctat
    11701 aatatatagg cgtttatttc tgtattaaac actttgatca taatcttcca gcagcaactt
    11761 tttttcgtac ttcatctttc caattgccac tatggattat tctatcgaaa ttacttcctg
    11821 ctcttcctga taataagtca attcgagata gcttgctgat atcatcccag ttatcataga
    11881 ggatatatat agcattacca tattctatgt tttcaaatac aaccaaatcg tcctccagca
    11941 tagctccaat ataattactg aacgtgctgt ttcctgtgac gattttttta ggttcaagac
    12001 tcatgaagaa gtcatatcta ttttcagcga catttttctt gtcggaagtg atatttttac
    12061 ctctaaaaat tcttgctaaa gtttcttctt tactacctgg tggaaatatt tcccatgaga
    12121 ctataagtgt atcagcataa gtcgatatgg gtttatcagc atcctcaatt ccacatttac
    12181 caagattttc ttgaagaaga tttaaatcaa ataataattc atcatcaaac ttatttgatt
    12241 gtttgtctaa aattctagtt actctgaagt taaatatgaa atggctgtcc gagggctgtt
    12301 tgtgattaat ttcaatggct atgagtgttg gtggtataat gtctctggga tatttctcgt
    12361 aaggtaatct aacagtatgt gtaccattag agctatcacc ccagttaggt gactcaattt
    12421 cagtgtaatg agtttctttt gggaggtctt ttctgacaat ttcttcacca aaaacatttt
    12481 taagtgaata tttaccctgc aatggatctg gtatgaccga cgtctcataa gcattaacac
    12541 cattatcaaa atatattcca aggtgctcta atgctccact ttttaattgg ttttccgtaa
    12601 atgcaataga agtgccagca acaactgtat taccaagcct tcttaacttg ctatgaatat
    12661 ttttaggtat aacacgtata ttctttttca tttttccctc atacttgatc atcaatgtct
    12721 ctttgctctc gcttgttatg taaatcattg ataaacataa catttgcaag tattacgcta
    12781 attactgcct caatctgcat gtacgaaata gcaataatta atgctaaaga tgtgtttttt
    12841 attacatctt ggctctctaa ataaagatga cttttgaaaa acactagttt aactaagtat
    12901 acaacaagta aagtaatcat aacgaaagat gattttagaa cacttccaac tattgcttct
    12961 aatcttcttg tatcttccag ttgttcggtg aaatcaacgg tttctatagt tttggggttc
    13021 acaatacgct gcaaagcatt tgggtaaaga aacgctaacc aaattcccat aatagtaaaa
    13081 accattccag aaactgatac gagaagagtg caatagttgg caaaatcgtt atacacatag
    13141 ttaatatcgg atgacgaaat aatcatcagc gatataatga atatcgccat gtatatccat
    13201 gtgtatttgg ctatgcctgc tatagctttc aatattcaat cctctccaga tgctttcttt
    13261 attcttggtt gagattgtaa tgcttttaat tcttttaata taggggaaat aaattctttt
    13321 cttttgtgtg atatttgttc ataaatatat gcggcggaat atgttgaata gtcgtcagaa
    13381 gggacattga gaatatcacg cattctatat ttatccaccc aagttatacc cttgtctgta
    13441 gcaaatccaa cattgtccca aagacgtttt tctctatcct cagccgaata cttttcaatg
    13501 atttcttgaa cttcattcaa ggatggtttt gcttctgctt ttatttcaat ttgtcttgtt
    13561 ttggcatttt tctttccgga cacaaatgga acgagagtgc taaaggtctt taaccaatca
    13621 gctcgtgcat catttggatt tataataata gtttccctgc gaactatgtg tgttattttt
    13681 ggtattagtt tacctaactc agtatgagtg gtttctaagc ttctgagctt catatcaaac
    13741 ctgtacatat atttatataa gtcttcgtca tcggtattag atagccttat ataacctttc
    13801 tcggttttat ttttaaccct tttgctatgt ttaactctat tagtaataga tgagtgtaca
    13861 tagtcttgaa atagttcaga atcacaaaca gaatggtcga atttaatgga tactatagtt
    13921 tcaagttcag gtataaccca ataaaagcat ggccgccccc agataacttt tttccctctg
    13981 tagctattag tatatttaac caccgatgag cttccgattt caccgtcttc actagctcct
    14041 aataaagagc ccgtactatc agtatcagat ttccataaca tgactaagaa atcaccagta
    14101 ttttcatcct tataaatatc cttgcaataa cactttgaac tgtttggttt ttcttttgga
    14161 tcccatggta tcgtgagtgc aagagattta gttcccaccc aatcctgtat gaggtcaaat
    14221 gtttcgctaa gttctaaacc ataggtattg tcatcattta ccttgtataa accgcactta
    14281 ttaaccttga agaaattgag atgccctaca ttcatgctct ttccttagct tcttatcttg
    14341 ggatatgtat catgatcaaa aattaagcat aaccccattg acttcgtgcg ggcaatgttt
    14401 tcaccctaga atttgatcgg tttaacgtta aatattcata agttacatga gcatttttca
    14461 cttcttccca attacagacg tcatgagctg aatattaata aaataattat cctcacggca
    14521 tggcgcgcaa tgctctcccc gtcacgcccg cccgctttgc agggcggttt taatgcagtt
    14581 gcacagtcac gctcaggctg cgccgggaat ggcgcggtct gcagaaaatg aggcaggaaa
    14641 acgcatgcaa agccatgcac cttatcgatg catggctttt ttcagtaaaa acgggcggat
    14701 tttcggggaa ttttacacag actgacgtga tgccagttgc gcacttttac gcgaaaaaat
    14761 catgttctgc gcaggggtga atttttcacg gctgtcatcc accgaagtcg cgtcaggcct
    14821 gaatccgatg gtcgttaaaa tgtcactatc ctgtgcggaa taattaattt tttccccctt
    14881 cgcaagccag gacagaaggg cttcacgcag ggcatctgtg gcacgctgta tggcacagtt
    14941 tcgggcaatg gccgtcagct cactgtagcc catcagctcc ggtgccagtg ccgccgccag
    15001 tgctgtgccg tgctgctgca taaaatcatt cagccggtcg cggatgctga tgtgctgaac
    15061 ggcttcatgc gaacgaatat aacgaccggc agcctgattc acctgccatt ttctgacttc
    15121 gataatattg cgtaattcgt ccaggcgact gacgtttctg ccttccccgg acagaagccg
    15181 cagatattcc tgttcggccg cagccagctc atttttgcgt tgcagccatg ctgctttgtt
    15241 attctgacag gtgtcaaagg cctgctgtaa ggctgtgctt tccatcgtta tctctttctc
    15301 atcatgctga agaataaaaa tacggtgtgc ggcgacggcc ggtgttaacc ggcagccctc
    15361 attccagacg cagcgaatat gattgtgttt ttaagcgtac tggcggcagt tcctgttttt
    15421 catgcaggct ttctgccagt tcgtccggcg tgaccgggcg gacaataaaa cggttgatgg
    15481 tctgaagcgt tttaaacacc agaccacagc ccggatccgt gcacacataa aaacgctcgg
    15541 tgacttcctg agacagacgc cgcgatgttc ttgacagtgc aaggccttta catctccgac
    15601 aacaatatcc ggtaacaagc attcttttcg ggcgtttcat actgccggag gctgacgtca
    15661 gtgaatcgcg gtatctctgt ttgcctgaaa tgtattccat tcctgaatct ttacagtcag
    15721 agaaaaagct ttcactcgct tcaaatgtcg cagagcaata aatattccgg cactgtgcaa
    15781 tcattatctt ggtgccatcg tccatgaaat gtgcgcgacg ggtgtgagca acatgtccac
    15841 acgacgggca gtaaatcatg acagcagtcc tctggcctta agctctgctc cctgctggtc
    15901 tattttgtcc tgccacacct tgcgctgtgc cggtgtgcct gccacctcat aatccatgtg
    15961 cgggagtgtt gccgctgaca gtccggtcag ccggagaacc ggctcgccgg tgaggctgat
    16021 ttgcatctgt ttaattttct gttccagcga tgatttcacc tgctgcatga cagccttttc
    16081 cggtgcgacg tagccctgat ggccggtggt gttggcgagc ggattttcct gtaccaggat
    16141 actcagatgc attgcccgga caagcgcctc acaggtttca ttcagggcgt gttccagctc
    16201 atgctctgca tacagactca gcaggtgatg atgtgccttc cggtaggcgg tggccgtgct
    16261 gtcacacgcc cctttcaggc gttcacgttc aaaattcagc accacggcca gattgtcata
    16321 ttcctgtacc agctcccggc gtgccacgcg ctcaatgtgg cgctgtttca gctcgtcgct
    16381 caggacacca ccggctgcac gaaaggccgt gcgccagtcg tcagcgtcat ttccgtcggc
    16441 ctgcgccagc gcattttttt cctgctctgc ccgttcaatg gccgtgacgg tctcatccat
    16501 caggcgggcg ttctcaaggt gggcggctct ggccttttcc agttgtgcca gtgcgggttg
    16561 cagatattca gggatggtgt tgtcagacat tttccggctc ctcgtcactt caggttaaga
    16621 aaattgtgac gtacaccgga caacaacacg acgcattgca gatgtgccag ccctgacaca
    16681 ggagactcat cctcagaccg gcaagccagg aaaaggtcgc aggaaaaacc ggcttactgt
    16741 ttgttttttt atattttact gttcacctct gttcaccata agaaaaaaga taagtaatac
    16801 agtaagttaa agggtgaaca atcgcagtaa tgactgttca ccgtctgttc accactgttc
    16861 acccgctcat agactttttg tgctgtttat tactttttat ttttattaat ccactgaaat
    16921 aaataagaaa aaacaatttg tatttcacta taattttttc cagctccttc cagagcgttt
    16981 taaagccatc tggcgctgat gtataaaaaa tacacagcca ttgtaagact gccggaacaa
    17041 atcccccctg ttgcatctgc caaaaatatt cacaaaataa agcgctaccc gaagccggac
    17101 ggacttatcc ggtgctgtat ggacattaac gaggtagccc gatgcaagct gttttttctt
    17161 ccccgtctcc cgcccctgtg acgccactga tgccgctgcc ggacatcacg caggagcgtt
    17221 ttttacgtct gccggaagtg atgcacctgt gcggcctgtc acgctcgacc atctatgaac
    17281 tcatccgtaa gggggaattt ccgccgcagg tgagtcttgg cggtaaaaat gtggcctggc
    17341 tgcactctga agtcaccgca tggatggccg gacgcattgc cggacgcaaa cgggggtacg
    17401 acgcatgatg atgcccgctc tgcaaaaact ccctttttct ggcttgcctt tttccggcat
    17461 ttgcggatat agtttttccg ctgccgcaaa atcggcagcc gggcgtgaga acccgagcaa
    17521 ttcacaggcg acatatgacg cgccacgcgt ctttttttgt gtcgcaatca atgccacaga
    17581 gcgccagatt atggtgtggt gtgtggtcag tcgtgcagat ggcattccgt gcatcctgcc
    17641 cgcgtccgct cattatgccg cagagtcaat ggtagctcag gcggggcagc ctccgggctg
    17701 gccggtatcc tgtgaagccg gtattctcac ccccgtctgg gctatcgcca tcgagcgtga
    17761 gaactccggc gatagcgtta tttgctattc acaggaggct gccatcatgg ctacaaccct
    17821 taccccctca caccctgaat ttgtctttgt gtttgcggct gtccgtcgcg cagaccgtca
    17881 tccccgtatc tgtatgcttc gcaccgtggc cggtgatgaa cgcagtgccc gccgttccct
    17941 tgtccgtgac tatgtgctct cccttgctgc ccgtctgccg gtggtggagg tgtcccgtgc
    18001 gtaataaaaa agcccctcag accgtctcag cacgtcatga cgcccgtgaa cacctcagca
    18061 ttgaggctta ccataagctc aaccgcgcca gcgccgtatc ccagtttgtt gggggtgatt
    18121 tgattcaccg tgaactctcc ggcctgcatc agctctacat tccgcatatt ttcagctacc
    18181 tgaatgaaga tattgatttt gtgctgaatg agctgaaagc caaaggcctg tgccgcgatt
    18241 ttctcgccca gcagaaagac cggggagaca ggacgcatgt ttgattttcc ccagcccggt
    18301 gagatttacc gttctgccgg ttttcccgat gtggccgtgg tcggcattct ggaagacggt
    18361 attccgtggg aaatgccgta ccgctgcccg gacattgtct ggaacccgta ccgccgtaaa
    18421 ttcagtatcc ttgtgcgtat cctcgccgac gggcgcacca cagacatccc gctggggcgt
    18481 tttctgcggg aatttacctg tgaccgtcct gacctgttta aacgcagccc cgtaaaccgg
    18541 catgcggtac tgaaagaaat ggccggagac ccggaattac agaaatggcg ggagaaatat
    18601 caggatattt acccgcagga cactgttccg gtcagccggg cagcaccggt agcgcgggaa
    18661 tggcgggaaa ttccccgcac ggagcctgac ccggaaatca ccccggataa cagttaccgc
    18721 aattatctgt aattaaaaaa cgacacccga aaaattaaat gtgcgtattc gcgcagggat
    18781 acgcacgtct tcaggagacg cagatatgcc ttatcagtta atgcaaccgg cacggaatgc
    18841 agtcatctgt cacagggagg aaagcaaatg aaaacaccct taccgcccgt cttacgcgca
    18901 gccctttacc gtcgtgctgt cgcctgtgcc tggctgaccg tgtgcgaacg tcagcaccgc
    18961 tacccgcatc tcacccttga gtcactggag gcggccatcg ccgctgagct ggaaggcttt
    19021 tatctgcgcc agcacggtga ggaaaaaggg cgtcagatag cctgtgccct gctggaagat
    19081 ttaatggaat ccggccccct gaaggccgca ccgtcgctgt cctttctcgg gctggttgtg
    19141 atggatgaac tctgtgcccg tcacataaaa gcgccggtac tgcactgaag gagaacaaca
    19201 ccatgaaaat gaacgtaacc gccaccgtca gccatgcgct cggccactgg ccgcgtattc
    19261 tcccggcgct ggggattcag gtgctgaaga accatcatca gccctgtccg gtctgtggcg
    19321 ggagtgaccg cttccgtttt gatgacaggg aggggcgcgg cacctggtac tgcaatcagt
    19381 gtggtgccgg tgacggcctg aaactggttg aaaaggtgtt tggtgtctcc ccgtccgacg
    19441 tggccgcaaa ggtggctgcc gtgaccggca gtctgccccc ggctgacccg gcagtgacgg
    19501 ccgccgccgt tgctgaaaca gacgctgccc ggaagaacgc cgccgcactg gcacaaaccc
    19561 tgatggcaaa aacccgtccc ggaaccggta acgcctacct gacccgcaag ggctttcccg
    19621 gccgggaatg ccggatgctg accggcacac acagagccgg tggcgtgagc tggcgcgccg
    19681 gtgaccttgt ggtgccactg tatgacgaca gcggcgaact ggttaacctt cagttaatca
    19741 gtgctgacgg ccgtaagcgc accctgaaag gcggacaggt caggggcacc tgtcacaccc
    19801 ttgaaggaca gaatcaggcc ggaaaacgtc tgtggatagc ggagggatac gcgaccgcac
    19861 ttaccgtaca tcacctgacc ggtgaaacgg tgatggtggc gctttcttcc gtgaacctcc
    19921 tttctctggc cagccttgcc cggcagaagc atccggcctg tcagattgtc cttgccgcag
    19981 accgtgacct cagcggtgac ggccagaaaa aagccgccgc agccgcagat gcgtgtgaag
    20041 gtgttgttgc cctgccgccg gtcttcggtg actggaatga tgccttcacg cagtacggcg
    20101 gggaggtcac ccgtaaggcc atttacgatg ccatccggcc accggctgaa agcccgttcg
    20161 acaccatgag cgaagcagag ttttccgcca tgagtaccag cgaaaaggcc atgcgtatct
    20221 atgagcatta cggcgaggcg ctcgcggtcg atgccaacgg ccagcttctg tcccgctatg
    20281 aaaatggtgt ctggaaggtg ctgccgccac tggactttgc ccgggatgtg gccgggctgt
    20341 ttcagcgtct gcgcgcgccg ttctcctccg ggaaggtggc ctccgtggtg gacaccctga
    20401 agctgattat tccgcagcag gaagccccct cccgccgcct gattggcttt cgtaacggtg
    20461 tgctcgacac acagaacggc acgttccacc cgcacagtcc gtcacactgg atgcgtaccc
    20521 tgtgcgatgt ggatttcacc ccaccggtgg aaggggaaac gctggaaacc cacgcccccg
    20581 cgttctggcg ctggcttgac cgtgccgccg gtggccgtgc ggaaaaacgc gacgtgattc
    20641 tggccgcact gtttatggtg ctggcaaacc gctacgactg gcagctcttt ctggaggtga
    20701 ccggtcccgg cggcagcggc aaaagtatca tggccgaaat cgccaccctg ctggccgggg
    20761 aggataacgc cacgtcggcc accattgaga cgctggaatc cccgcgtgaa cgtgccgcgt
    20821 taactggctt ctcactgata cgcctgccgg accaggaaaa atggagcggc gacggtgccg
    20881 gactcaaggc catcaccggc ggcgatgcgg tgtccgtgga cccgaaatac cgggatgcgt
    20941 actccacgca tatcccggcg gtgattctgg ccgtgaacaa taacccgatg cgcttcaccg
    21001 accgcagcgg cggcgtgtca cgccggcggg tgattattca cttcccggaa cagatagccc
    21061 cgcaggagcg cgacccgcag cttaaggaca aaatcacccg cgagctggcg gtcatcgtgc
    21121 gtcacctgat gcagaagttc agcgacccga tgctcgcccg gtcactgctt cagtcccagc
    21181 agaactcaga cgaggcgctg aacatcaaac gggatgccga cccgacgttt gattttatcg
    21241 gctatctgga aaccctgccg cagaccagcg gcatgtatat ggggaacgcc agtatcatcc
    21301 cgcgtaatta ccgtaaatac ctctatcacg cctatctggc ctacatggag gcaaacggct
    21361 accggaatgt actcagtctg aaaatgttcg ggctggggct gccggtgatg ctgaaggaat
    21421 acggactgaa ttacgagaag cgccatacca aacaggggat acagaccaac ctgacactga
    21481 aagaggaaag ctacggcgac tggctgccaa aatgtgacga ccctgcaaca acctgaccca
    21541 cctgaccggc atctgccggt ctttttttat cccgacctcc cccgaaggtg aacaatccac
    21601 tgttcaccct tcaccgtata ttcacccgtt atcacactga aattaaaaga gaaaaatgaa
    21661 aggtgaacag tgtgaacagt tctttcgaaa aaaaattttt tatgtggata aaagatgcac
    21721 tggtttggat ccgggctacg gatccaaact tactgattaa aaattattta actttccgat
    21781 ttattgatgg gctgaaaata gattggtcag aaaaacagtg ggggcacaaa agggggcata
    21841 tttcattgtt gtgttttatt atttgttttt tatcattaac ttattttgat atttgagtcc
    21901 ggccttcgca ccaaaagtat gtaaatagac ctcaactgag gtcttttttt atgcctgaaa
    21961 tccgcgctac acaaggcttt tccagctttt cccctcaact gaagtcaacc tgactcaacc
    22021 cacatcaaca ggcttgtgag tatactaatg agtatatttg ccgattcgat attgcttgta
    22081 tactcacgag ataccaatgg aaggaagacc attatggccc taacggatac taaagttcgc
    22141 tctgcgaaac ctgaggagaa agagtattca cttgttgacg gcgacggcat gtccttactt
    22201 gtaaaacctg gcggttcaaa gtattggcga tttcgtttcc gttttggcgg taaacaacat
    22261 ttgatggcgt tcggcgttta cccggatgtt tcgctggcgg atgcgcggaa gaaaagggaa
    22321 gaggccagaa agctggtagc tgctggtatc gatcctcgtg agcataagcg tgctgtgaaa
    22381 gaagagcagg cgaaagagat tattactttc gagaaggttg ccagagagtg gctcgtaacc
    22441 aaccaaaaat ggtcggaaga gcatgctaat cgtgtaaaaa agagcctgga ggacaatatc
    22501 ttcccggcaa ttggttccca caatattgct gaactgggca cccgcgatct gttaattcct
    22561 attaaagccg tggagaaatc cggacgtctt gaagtggctt cccgccttca acagcgcacc
    22621 acggccatca tgcgttatgc agtgcaaagc gggttaattg attacaatcc ggcgcaagag
    22681 atggcggggg cggtagcttc cagtaatcga cagcatcgtc cggcgctgga gttaaagcgt
    22741 atcccggaat tactccagaa aatcgatgaa tacaccggca ggccactaac ccgctgggca
    22801 accgaactta ccctgcttat ttttattcga tccagtgagc tgcgttttgc tcgctggtca
    22861 gaaatcgatt ttgaaacgtc aatgtggacc atcccgcctg agcgagaacc tattcccggt
    22921 gtgaaacatt ctcagcgagg agcaaagatg cgtacacctc atctggtgcc gctttcaaag
    22981 caggcgctag cgatcctgaa gcagataaaa cagttctgtg gcgaacatga gctgattttt
    23041 attggcgatc acgatccgag taagcccatg agtgaaaaca cggtgaacag tgcattgcgg
    23101 gtgatgggct atgacaccaa ggttgaggtt tgcggccacg gcttccgcac tatggcctgt
    23161 agctcattga ttgagtcagg gttatggtcg aaggatgcgg tggaaaggca gatgagccat
    23221 atggaacgca attcggtgag ggcggcgtat atccataaag cggaacatct ggatgagcgc
    23281 aagctgatgt tgcagtggtg ggctgatttt ctggatgcga accgggagaa ggggattacg
    23341 ccgtttgact atgcgaagat caatcggggt aatggcgagt gagttatcta aaagcgcaca
    23401 ttcgcctcta aattgaaagc tgctgactta ttcaaacgag gaattggtca gcagtgccaa
    23461 atagccgagt tttcactaag gtaagccttt ttattgaacc taatcggcag taatgctcca
    23521 cctaagccta aatttaaagt catgattact tataaaaggg gagtggaccc ttgattgagc
    23581 cctacatcgt aacaaaggta cagcaattcg gctacatcag gagtgatagt caattagtta
    23641 atctaataat taattgaatc taaataatta aatttcttac gtaaaattga ccaatttact
    23701 ggtggtgtaa gccgcaatat cttaatgtaa tcagtataaa ggggcttgat ttcttttaaa
    23761 gatagtatcc gtatgaattt taagaaaaaa ctcgaagagc attttaagca gttcgaagca
    23821 tccccagtgt tatttgtcgg ctccggtgtg tctcgtcgtt atcttggtgt gccatgttgg
    23881 caagaccttt tgaagcattt tgctgaggca attggggaaa atcatataaa attaaaaaca
    23941 aaatctaatg gtgacttacc cgaatatgca caattattgg tgtctgcata tgctgagaaa
    24001 tggtgggata ctgaagaagg acaattagca ttaagtgaaa aagaacagga gaaaactttc
    24061 attaacgaac agtctccttt gaagttgtca atatctaaat atattgagaa tgcacataaa
    24121 aatattatag ataatgatga gttaaaacat gaaa
//
LOCUS       DAJTOR010000048        23919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTOR010000048 DAJTOR010000000
VERSION     DAJTOR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.048"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..246)
                     /locus_tag="OO249_001715"
     CDS             complement(<1..246)
                     /locus_tag="OO249_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR5636053.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTI"
     gene            complement(395..811)
                     /gene="sufE"
                     /locus_tag="OO249_001716"
     CDS             complement(395..811)
                     /gene="sufE"
                     /locus_tag="OO249_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5636054.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(824..2044)
                     /gene="sufS"
                     /locus_tag="OO249_001717"
     CDS             complement(824..2044)
                     /gene="sufS"
                     /locus_tag="OO249_001717"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5636055.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAEYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(2041..3312)
                     /gene="sufD"
                     /locus_tag="OO249_001718"
     CDS             complement(2041..3312)
                     /gene="sufD"
                     /locus_tag="OO249_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5636056.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTSLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(3287..4033)
                     /gene="sufC"
                     /locus_tag="OO249_001719"
     CDS             complement(3287..4033)
                     /gene="sufC"
                     /locus_tag="OO249_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5636057.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPAEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(4043..5530)
                     /gene="sufB"
                     /locus_tag="OO249_001720"
     CDS             complement(4043..5530)
                     /gene="sufB"
                     /locus_tag="OO249_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5636058.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(5539..5907)
                     /gene="sufA"
                     /locus_tag="OO249_001721"
     CDS             complement(5539..5907)
                     /gene="sufA"
                     /locus_tag="OO249_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5636059.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(6454..6642)
                     /locus_tag="OO249_001722"
     CDS             complement(6454..6642)
                     /locus_tag="OO249_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5636060.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(6742..7152)
                     /gene="menI"
                     /locus_tag="OO249_001723"
     CDS             complement(6742..7152)
                     /gene="menI"
                     /locus_tag="OO249_001723"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5636061.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(7149..10205)
                     /locus_tag="OO249_001724"
     CDS             complement(7149..10205)
                     /locus_tag="OO249_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5636062.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDVLDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPELDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            10594..11706
                     /gene="ydiK"
                     /locus_tag="OO249_001725"
     CDS             10594..11706
                     /gene="ydiK"
                     /locus_tag="OO249_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5636063.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            11892..11999
                     /gene="rprA"
                     /locus_tag="OO249_001726"
     ncRNA           11892..11999
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO249_001726"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            12135..12514
                     /locus_tag="OO249_001727"
                     /pseudo
     CDS             12135..12514
                     /locus_tag="OO249_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            12459..13181
                     /locus_tag="OO249_001728"
                     /pseudo
     CDS             12459..13181
                     /locus_tag="OO249_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13193..14059
                     /gene="ydiB"
                     /locus_tag="OO249_001729"
                     /pseudo
     CDS             13193..14059
                     /gene="ydiB"
                     /locus_tag="OO249_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
     gene            14091..14849
                     /gene="aroD"
                     /locus_tag="OO249_001730"
     CDS             14091..14849
                     /gene="aroD"
                     /locus_tag="OO249_001730"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5636064.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            14995..16590
                     /locus_tag="OO249_001731"
     CDS             14995..16590
                     /locus_tag="OO249_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCR5636065.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFNFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIQELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            16604..17755
                     /locus_tag="OO249_001732"
     CDS             16604..17755
                     /locus_tag="OO249_001732"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCR5636066.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(17798..18709)
                     /locus_tag="OO249_001733"
     CDS             complement(17798..18709)
                     /locus_tag="OO249_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5636067.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLSNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            19023..19794
                     /locus_tag="OO249_001734"
                     /pseudo
     CDS             19023..19794
                     /locus_tag="OO249_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            19814..20752
                     /locus_tag="OO249_001735"
     CDS             19814..20752
                     /locus_tag="OO249_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5636068.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLVATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            20808..22097
                     /locus_tag="OO249_001736"
     CDS             20808..22097
                     /locus_tag="OO249_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5636069.1"
                     /translation="MSDDKFDAIVVGADVASSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYSRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            22094..22387
                     /locus_tag="OO249_001737"
     CDS             22094..22387
                     /locus_tag="OO249_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5636070.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            22444..>23919
                     /gene="fadK"
                     /locus_tag="OO249_001738"
     CDS             22444..>23919
                     /gene="fadK"
                     /locus_tag="OO249_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5636071.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSPIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYCSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLE"
BASE COUNT         5878 a         6088 c         5993 g         5960 t
ORIGIN      
        1 tatcgtcgta ccaggttttg gcaccggggc gatagtgtta ttggcttcaa ggatcaacat
       61 tgccgcagta tcaaaacgtc gggcaatagc ctgaaggttt ttatcccctt cttgcaccgt
      121 atacgtttga ttttgcccaa ccagtcggct tccggttggt ggtagcggat aatcaaccgc
      181 ccaggcagcc tggatggcgc taaaagcgcc gataagcgtg agtgtaagca aagacgcgcg
      241 tttcattgta aacctcctgt atttgccgga gactcacgct gaaacgtcgg atggcgctta
      301 tgttcacctg aaaccaaaac actcctgtgc aggtcagtgt aaacattgac catccggcaa
      361 tgtgagccaa ccggatgaaa gctgtccctt tagtttagct aagtgcagcg gctttggcgc
      421 gaattgcgcg aatcatcgct tccagacctt gtgaacgaga tggggtgaga tgttgggtga
      481 gcgccatttt ttcaaaccac ggacgcacat cgaaattgac aatatcctgc ggcgtcatct
      541 gatcgtagag aataaatacg accgcaataa gccctttcac aatcgccgca tcgctgtcgc
      601 cctgcaattc aataattccc tgcgcattct ggcgcatgac aatccacacc tgattctggc
      661 agccctgaat gctattttgt gggcttcggt cttcgtcgcg taattctggc agacgctggc
      721 ccagctcaat aatgtagaga tatttctctt cccagttggc gcagcgtaaa aaattacgca
      781 gcaatttttc tttatccggc aataaagcca tagtgcctcc ctgttatccc agcagacggt
      841 gaatacgttg caggccggtc accagacgat ccacttcttc atgagtgtta tacatggcca
      901 gcgacgcccg acacatcgca gggacgttgt aataggccat taatggcata gcgcagtgat
      961 gtccggtacg cacagcaatg ccgtaattat cgagaaaact gccaacatca taggcgtggt
     1021 gtttaccgag attaaaagca ataacgccaa gcctgttttg tgggccatag agagtgagat
     1081 tcggtacaga ttccagctgt gatagcgcgt aatgcatcag attcagttca tactcggcta
     1141 tgttattaag ccccagcacc gaaacatatt ccagcgccgc gccaagacca atgatgcccc
     1201 cggtattggg tgtaccggct tcaaaccgcc atggtgcttt ggtccaggta gtgccttcac
     1261 tcaggctgac ggtggcgatc atagaaccgc ccccttccca cggcggcatc tcctgcaaca
     1321 aggcttcttt gacataaaga atgccaatcc cggtggggcc atacagttta tgcccggaaa
     1381 acacgtaaaa atcgcaatcc agcgcctgaa catccaccgg atgatgcatc accgcctgag
     1441 cgccatccac cagcactttt gcgccatgct ggtgcgcaag cgtgatcatt cccgccagtg
     1501 gattttctgt gccaagcacg ttggagacat gagtaattgc cagcaggcga gttttctcat
     1561 caaacagcgt atgcagcgtc tccagttgca gcgtaccgtc ggggttgaga gggatcacac
     1621 gcagctctgc gccaacgcgt gcgcaaagca tctgccaggg gacaatgtta gcgtggtgct
     1681 ccatctgact gatgatgatg ttatcgcccg cccgcacatt gctgttgccc cagctattgg
     1741 cgaccagatt gatcccttcc gtcgtgccgc ggacgaacac cagctcttcc gccgaacggg
     1801 cattaataaa cagcgatgcc cgcttgcgca cgttctccat tttctcggtc gcctgggcgc
     1861 ttaaggtatg aataccgcga tgcaccgccg cgtagccatg acgataaaac tcggcctcgg
     1921 cgtcaatcac ctggctcggt ttctgtgcac tggcggcgct gtcgagataa gccagcggca
     1981 aaccgtttac ctcacgcgaa agcaccggaa agtcggcccg cactttgtcg acggaaaaag
     2041 tcatcttgca cctcctggca gccgttgacc gattcgggcc agcacctgct gtttaagccc
     2101 ctcatcacgc agtgcttccg tcagttcggc agcaaaggcg taaatgatca tctgctgggc
     2161 atcctgctga ttgatcccgc gcgagcgcag atagaacatc tgttcatcat caatacgccc
     2221 caccgtcgcg ccgtggctgc atttcacatc atctgcatag atttccagct gcggtttcgt
     2281 atccacttcc gccagtttgc ccatcagcag attattgttg gtcatctgac catccgtttt
     2341 gatggcgtgc tgcgcgacgt tgatcaaacc gttaaatacc gcgcggcctt tgtcgctgac
     2401 gatagttttg tgcaactgtc ggctgttaca aaaaccttta ttgtgttcca gccaggtacg
     2461 ggtatcacac acctcgtttt tcaccggcat cgccaggcta ttgatccgca gcgtgctgtt
     2521 ttcgccattg agttgcgtac tggtgttgtg tcgtaacact gcgccaccca gcaggaaact
     2581 atggctaaat gcggtggcat cctcagccag caacaaatcg ttatgagcaa agtggtgact
     2641 gagcgggttt tcaaacgcca gcttgatatg ctgcaagtgg gcgttcgctg cgacattgat
     2701 agtgaaccgt gccccggtaa aatgacgagc atcattaagg ctgacaaaat gttcgatcac
     2761 cgttgcttcg gcaccttccg ccagatccag atgatgccgg taatgggcag tgttcacctc
     2821 ttcacctgcc acgccctggg tgatatacat taacagcaat ggctttgccg gccgttgacc
     2881 gcgcttcacg gcgatatgcg tcacgctttg tgccaggctt tccgtcaaat gcagaaacac
     2941 ttccgcctga atagcgtcgg gtaacccctg acggtcgtcg ttaatgctca cttcatatcc
     3001 gctgccttca attgcatcgc tcagcgcggg cacgtaacgt ccatcgacaa acaccagccg
     3061 cacggcgtct aacgttaacg ctaaggcatc acgctgctgt ggggatatct cccccgcaat
     3121 gctgacaaac tggctattgg tcagcccttc cagcgacgta tatttccagt tttcatgttt
     3181 acgtgtcggc agtccggtac gcagcaattg ttgtaaatgc tgctgtgctt gcggggagcg
     3241 ttttgtccct tcagcttcaa acaagtgatg ccactgttgc agcgcgttac tgctgttcgg
     3301 taagccagcc ataaccctgc tcctccagtt gtttgaccaa cgtgaaatcg ccggatttca
     3361 caattcgtcc ctgatatagc acatggacgt aatcaggctt gatgtagtcg agaatgcgtt
     3421 ggtagtgcgt aacaatgatg aatgagcgct tgccatcacg cagggagttc acgccatcgg
     3481 cgaccacttt taatgcgtca atatccagcc cggagtccga ctcatcaaga atgcataact
     3541 ccggttccag caccgccatt tgcaaaatat cgttgcgctt tttctcgccg ccggaaaaac
     3601 caacgtttac cgaacgggtt aataaatctt ccggcatctt caggagagcg attttctctt
     3661 ccatcaaatc ctgaaaatca aagcggtcga gcgtttcctg gccgcgatag ctgcgcactg
     3721 cattaagtgc cgtttgcagg aaaaactggt tactgacgcc tggaatctcc gccggatact
     3781 ggaaggccat aaagatgcct tcgcctgcac gatcttccgg cgacagtgca agcaaatctt
     3841 tgcctttgaa ctcaaccgtg ccgcccgtca cttcataatc ttcgcgcccg gcaagcgttg
     3901 ccgataaggt acttttgccc gaaccgtttg gccccataat ggcgtgaact tcgccgggac
     3961 gaacggcgag gcttaatcca cgcaggatag ctttatcttc cacgctgacg tgtaaatctt
     4021 taatacttaa catgtttatt ccttatccga cgctgtgttc aagactgatc gcgaggagtt
     4081 tttgtgcttc aacggcaaat tccagcggca gttctgagaa cacgtctttg cagaaaccgt
     4141 taacaatcat cgagatggcg tcttcttcgc tgatcccgcg ttgcaggcag taaaacagtt
     4201 gatcttcacc aatacgtgat gtcgttgcct cgtgctccag ttgtgcgctg ttattgcgac
     4261 attcgacata cgggaaggta tgcgccccac aattagcgcc aatcagcatt gagtcgcact
     4321 gagtgaaatt gcgcgcattg gttgccgtcg gcatgatttt cactaagccg cgataactgt
     4381 tctgactatg tccggcagag atccctttcg agataatggt cgatttggtg tttttaccga
     4441 tgtggatcat cttggtgccg gtatccgctt gctgatgacc gctggtcagc gccactgagt
     4501 aaaactcacc aatggagtta tcgccgcgca aaatgcagct gggatatttc cacgtaatcg
     4561 cggacccggt ttctgattgc gtccatgaca ttttgctgtt ttcgccttcg cacaaagcac
     4621 gcttggtgac gaagttgaga ataccgccgg tgttgttatc gccagggaac cagttttgta
     4681 ccgtggaata tttcacctcg gcgtttttat ggatgatgac ttccaccact gccgcgtgta
     4741 actgatagct gtcacgcacc ggagcggaac agccttcaat gtagctgacg tagctgtctt
     4801 cgtcggccac cagaatggtg cgctcaaact gcccggtttt ttccgcgtta atgcgaaaat
     4861 aggtggaaag ttccatcggg cagcgcacgc ctttaggcac ataaataaac gtaccgtcag
     4921 aggctaccgc cgcattaagc gcggcaaaga agttgtcatt ccccggcacc acggtgccga
     4981 gatatttacg caccagttcc gggtgatcgt ggatcgcctc accaaaggaa cagaaaataa
     5041 ttccctgctc cgccagtttt tcgcgataag tagtggcaac cgaaacagag tcgaaaatgg
     5101 catccaccgc cacctctttg ccttcccgca cgggaacgcc caactgctca aacgccgcct
     5161 ccacctcttt acttaaaaag gcgttcgcgc cagtttgctg caccgcgcca ggttcagacg
     5221 cgcaagtgtc gtcacaatta ccgcacgatg gtgctgagta gtagctgtaa tcctgataat
     5281 tcagcttgtc gtagtgcgct ttcaaccagt gcggttcttc catctccagc catgcgcgat
     5341 aggcgtttag acgaaactcc agcatccact ccggctcatt acgcttcgcc gaaattgcgc
     5401 gcaccacctc ttcgtttatc ccctttgcca gctcatcggt ggctaactgg gtgaagaatc
     5461 cttctttata attcagcggg ccgccggtcc aggttttgac atcgtcagtt gcttcagtat
     5521 tacgagacat agtaccgcct ataccccaaa gctttcgcca cagccacatt cattctgggc
     5581 tttagggttg tgaaatttga atatctgatt aagtccttca cgaacgaaat cgacttccgt
     5641 gccatcaata aacggcatcg cttgcagcgg gacaaacagc ttcgcgccgt cgtgttcaaa
     5701 cagcagatcg tctttgtccg gctcgctaac actgtcgagc acatagccaa agcccgcgca
     5761 gcccgtttgc ttcacgccta aacgcacgcc gaccataccc ggctgctttg ccaccagctc
     5821 acggatgtgt atcgccgctg cgggtgtcag cgttaagcct tgccaggcga aatcttgtgg
     5881 gttaaaggtt cctgaatgca tgtccatcga tttacctcac ttcatcgctt tcagcgtata
     5941 acagcatgtt ggtgataatg attatcagtt caacccagca aacgcagggg ctttaccgta
     6001 aaacatgctt tttgcctgct tttaataagc atagaccctg atgtgtgggt taacaggcac
     6061 gctaagaatt aggtatctca ttgttagata atggttatta tctaaggagc attaaggctg
     6121 tgatgaaaaa gaaaaagttg tattaaaatg actatttaag aaataggtaa aaaagtgcag
     6181 cgttcagaaa taagaaaacc cttaagtctg tgcgacacag gcttaagggt ttctacccca
     6241 tccggcgctt atctccggca ctctcagtgg cttagctctt gaaggggcga taagaataat
     6301 ctcataaagc taacccgccg ttttaacaca aactgcgatt agtattattt ttgaacaata
     6361 tcaggcggta gataagcagt attaagaagg tcatcgaacc tggacggagg ttaatccagg
     6421 tcgatttggc gaacttgcgg cattaagtca ggatcaatgc acgcccagac gccaggcaaa
     6481 gtagatttct tcttttaatt cagcagaaga gagagtaagc aggtcagcaa attcaagttc
     6541 tagttgtttc agacgtttga gatattgggc aggtgaaaga ttgctctggt cacggcgtaa
     6601 aaattcaatg gccagctggg tgggatcaag ttgagtagac atagcatcct cgcttttaga
     6661 caagacgtgt acagtatacc accgtttact gtgcagataa tgaccaaaag caatatgcgt
     6721 cacacttttc tggtgacaac gtcacaaaat ggcggtcgtc aatcgtgacg aacagcacaa
     6781 acgccctttc tcatcgaaga tttcaatctg ccagacctgg tgacgcgaac cgagatgcaa
     6841 cggtttacat atgccgcgca cccgcccttc tcgtgccgag cggacgtgat tagcattgat
     6901 ttccagacca accacttttt gctcaccttc ggtacataaa taaccggcaa cggaaccgat
     6961 actttcggcc agtaccacgg atgctcctcc atgcagcaac ccgaaaggct gctttgtccg
     7021 cgagtctact ggcattgtcg cttcaagggt gtcatcacca atatgttcaa agcgaatatc
     7081 caggaacccc accatgtttc cttcacccat agcattcagt gcttccaggg tgattttccg
     7141 tttccatatc atttaataat ctccagtaaa gcctgcacag gatggcgtac ccccgtgcct
     7201 tcaacccgtt ttacctggct acggcaggaa tatccggtcg ccagacagcg gttacgcggc
     7261 agtcgctgca tagcctgatg ccaggataac tcatagatcc cgagcgaatt ttcatggttt
     7321 ttcgcttcat gtccgtaagt ccctgccatg ccgcagcaac ccacgctgac attttccagt
     7381 ttcgcgccaa aacgggcaaa tatcgcggcc cattgtgctg gcgcacccgg caaggcggta
     7441 acttcggtac agtgaccaaa gaaataccat gattcaccgc tgactgtagc cacaggctgt
     7501 gactcaagtg cgcttgccag ccattcattc gccagtaaga cgttaaactc gccacgctcc
     7561 tcgcccagcg ccagtttata ttcatcgcga taacaaagta ccagcgccgg atcgacgccc
     7621 accattggca tacccagctt cgccatacgg ttgaggaaat ccgccgtctt tttcgccgtc
     7681 ttcgcaaaac gattaagaaa acctttaata tgctgggctt tgccatttgg cgaaaatggc
     7741 agtaacacag gctggaaacc taatttttcg accagacgga caaaatccgc caccacttgc
     7801 gcatcgtaat agctggtaaa ggggtcctgc accaccaaca ctgtgcgcgc tttctgctct
     7861 gcattgagag attcaagctg ttccagcgtc atgtttgccg agcgatgccc caccatttgt
     7921 tgttgtagcg aggggaccga cagcagcggc aaatcaacca tgccgatatg tttttccgag
     7981 agtttgcgca ccagcggctg gttaatgaag aagttaaagg ttttcggcgc gcgtgccatc
     8041 agcggcgcgt agctctcgac cgtagcgacg aggtggtcgc gcagcgggcg taaataacgg
     8101 gtgtgataga gctgcagaaa acgagagcga aactccggca catcaatttt gatggggcac
     8161 agggtcgaac acgctttaca ggccagacag cccgacatcg cctctttgac ttcgtgtgag
     8221 aagtcatatt cgcctttatt cgcatgccag ctattgcgcg tgcgggcaat taacgtccgc
     8281 aaactgacgc cggattcagg cagttcttgt tccagtttga gtggatcaac gccgcgatcc
     8341 gccaacaaac gcagccattc acgcaccagc gttgcgcgcc ctttcggtga atgaatccgg
     8401 ttctgggtga tcttcatcga cggacacatc ggactacggg catcaaagtt gaagcataaa
     8461 ccgttgccgt tacactccat cgcaccgcgc cactgctggc gtaccgcaat ggggatctgc
     8521 cgatcgaatg taccgcgctt caccgcgtcc actttcatca tcggcgcatc gagaccttct
     8581 ggcgggcaaa tcttccctgg gttgagtcgg ttatgcgggt caaatgccgc tttcactttg
     8641 cgcagttctg caaaaagttc ctcaccgaaa aacgccgggc tgtattcagc gcgaaaacct
     8701 ttgccgtgct cgccccacaa caaaccaccg tatttcgcag tcagcgccac cacgtcatca
     8761 gagatttgct tcatcaaaat ctcttgttga ggatcgcaca tatccagctc tggacggacg
     8821 tgcaagacac ctgcgtcgac gtgaccgaac ataccgtagc ttaagccgtg gctgtcgagc
     8881 agcgcgcgaa attcagcaat ataatccgcc aggtgttccg gcggtacgca ggtatcctca
     8941 gcaaacggaa ttggcttagc ggcacctttg gcattgccaa gcagaccaac ggcttttttg
     9001 cgcattgcat agatacgttc aacccccgcc agctcacggc acacctgcca gccgatgaca
     9061 cctgcttgat gactggcgat cagctcatcc aaccgcgcac agagtgcatt tacccgctca
     9121 tcaatcagcg cctcatcatc accagcaaat tccacaatgt tcagcccgag catctcttgg
     9181 tcaggcacat cggtaatcaa ctcgctgacg gaatgccaga caatatcttc ccgcgccaga
     9241 ttcagcactt ttgagtccac cgtctctacc gaaagcgccc gcgcctcaac cataaacggc
     9301 gcgttacgca gcgcagagtc aaaagagtca tatttgacgt tcaccagacg gcgcacttta
     9361 ggcaagcgcg taatatccag ccgcgcttcg gtaataaagg ccagcgtccc ttctgaaccc
     9421 gtcagaatgc gcgtcaggtc gaactcggtc atctcatcgt taaagacatg acgcagatcg
     9481 taaccggtaa gaaagcggtt aagtttgggg aagttgtcga taattaactg gcgttgctga
     9541 cggcaacgtt gataaaccgt gttataaatt cgcccgattg tggtattgga tttacccagc
     9601 gtttccgcca attcgacggg taaaggttgc gtatcgagaa catcgccccc caacaacacc
     9661 gcgcgtacgc caagtacgtg atctgacgtt ttgccataga ccagcgatcc ctgaccggat
     9721 gcatcggtat tgatcatccc accgagcgtt gcccggttgc tggtcgaaag ttccggcgca
     9781 aaaaagtagc cgaacggttt caggtactga ttgagttgat cttttatcac cccggcctca
     9841 acgcgcaccc agccctcttc agggttaatt tcgatgatgc ggttcatatg gcgggacata
     9901 tcaacaataa tcccctggtt gagcgcctga ccgttagtgc cggtgccgcc gccgcggggg
     9961 gtaaagatca gcgatgaata gcgttcctgc gcggcaagac gggcgatcag cgccacatct
    10021 gcggttgaac gcggaaatac caccgcatcg gggagaagtt ggtaaatact gttgtcggtc
    10081 gacattgtca gacgatcggc ataacttgtc gccgtatcgc cggtaaaacc ttgttgctcc
    10141 agctcttgca aaaaattaag caccagttga acgacgccgg gtgcctgaga aatctgtgga
    10201 atcattatat tgcccctttc ctgcggtctg tgatgtaggt cgatacacta ttctttcagg
    10261 cggctgcaat agcgcactga aaggtgatgt ttatttactc tatggatttc gagttgcagg
    10321 aaggcggcaa gcgagtgaac tccaggagct tacaatagta agtgactggg gtgaacgaac
    10381 gtagccgcag cacatgcaac ttgaaatacg acgagtaaat cgtttgcgtg ttgcctgagt
    10441 tgttgtacca catttttttc taacacgccc atcagaatta agggcagaat cggcctgtta
    10501 aaaaccgctg aaattgctca tcattatgca ggtgagtttc gcgtgttcac gtcgcgtcga
    10561 cgatttgacg cacaaaaaag gtgaaaagta gttatggtaa atgttcgtca gcccagggat
    10621 gtcgcacaaa ttctgctttc ggtgctgttt ttagccatca tgattgtggc atgtctgtgg
    10681 attgttcaac cctttattct cggctttgca tgggccggta cggtggttat cgccacctgg
    10741 ccggtattgt tacgtttgca aaagatcatg tttggccgcc gctctctcgc cgttctggtg
    10801 atgacgctgt tattagtgat ggtgtttatc atccctattg ctttgctggt taacagtatc
    10861 gtcgacggca gcggcccgct aattaaagcc atttccagcg gcgacatgac gttacccgat
    10921 ctggcgtggc ttaataccat tccggtgatt ggcgcgaagc tgtatgcagg ctggcacaac
    10981 ttgctggata tgggggggac ggcgatcatg gcgaaagtcc gcccttatat tggcaccacc
    11041 accacctggt tcgttgggca ggcggcgcat atcgggcgct ttatggtgca ttgtgcgctg
    11101 atgcttctct tcagtgccct gctgtactgg cgcggtgaac aggtggcaca aggcattcgc
    11161 cattttgcaa cccgtctggc aggcgttcgc ggagatgccg ccgtactgct ggcggcacag
    11221 gctatccgcg cggtggcgct gggtgtggtg gtgacggcgt tagtacaggc agtgcttggc
    11281 ggtatcggcc tcgccgtatc cggcgtacct tatgcaacgt tgctaacggt gttaatgatc
    11341 ctctcctgcc ttgtccagct tggcccgttg ccggtactga ttccggcgat tatctggctc
    11401 tactggactg gcgataccac ctggggaacg gtattgttag tgtggagcgg tgtggttggc
    11461 acgctggata acgtcatccg cccaatgtta attcgcatgg gtgccgattt accgctgatc
    11521 ctgatcctct ctggtgttat tggtggtttg attgctttcg ggatgatcgg tctgtttatt
    11581 ggtccggttc tgttagccgt ttcctggcgt ctgtttgccg cgtgggtgga agaagtcccg
    11641 ccgccgacgg accaaccgga agaaattctc gaagaacttg gcgaaatcga gaaaccgaat
    11701 aagtaatttc tcatcaggcg gctctgccgc ctgattgtta acccccgcta attgatattt
    11761 gcttgctctt ccccatattt tcctgcttac cgccattcag ctggtagtac ctgtcgcaaa
    11821 ttctttacag tttttaaact aatgagacga atctgatcga cgcaaaaagt ccgtatgcct
    11881 actattagct cacggttata aatcaacata ttgatttata agcatggaaa tcccctgagt
    11941 gaaacaacga attgctgtgt gtagtctttg cccatctccc acgatgggct tttttttaac
    12001 atttttccac ttcgctacct cgcccctcac tctttccaat aatgttgcac aagatactgt
    12061 gactctcttc aagccgtcag caggctgata atgttattta tattttattg tttatttcat
    12121 gcagccatta cacgatgaac gcttatgaac tccaggcact ccgacatatt tttgccatga
    12181 ctattgatga atgcgcacct ggattgccca gaccggtgac agtgaaagct ggcgtcagtg
    12241 ggaaaatggc aagtgtgcca ttcctgatcg tgtagtcgag caactgttgg ctatgcgtca
    12301 gcaaagaaaa aaacatcttc atgccatcat agagaaaata aataaccgca tcggcaacaa
    12361 caccatgcgt tttttccccg acttaaccgc atttcaacga gtctaccctg atggcaattt
    12421 tattgactgg aagatctatc aatcggtagc cgctgaaggt aatgctgttg aaaagcaaat
    12481 tccccagcca gttggtggac gccagcgtag ctaatgaatt accgcaaatg aacagcaaac
    12541 cgttagtctg gctggaaggt gtttcatcgg tactgttcgg tgtagccgca ttctcgacct
    12601 tttatgtgat tgtggtgtgg atgcccaaat atgcgatggc ttttgctggt atgtcagaag
    12661 ctgaggcatt aaaaaccatc tcttattaca gtatgggctc gttggtctgt gtctttattt
    12721 ttgccgcact actgaaaaaa atggtccggc ccatctgggc taatgtattt aactctgcac
    12781 tggcaacaat aacagcagcc attatctatc tgtacccttc tccactggtg tgtaatgccg
    12841 gcgcctttgt tatcggtttc tcagcagctg gcggcatttt acagctcggt gtttcggtca
    12901 tgtcagagtt tttcccaaaa agcaaagcca aagtcaccag tatttatatg atgatgggtg
    12961 gactggctaa ctttgttatt ccgctgatta ccggttatct gtcgaacatc ggccttcaat
    13021 atatcattgt tctcgatttt actttcgcgc tgctggccct gattaccgca attattgttt
    13081 ttatccgcta ttaccgcgtt tttattattc ctgagaatga tgtgcggttt ggcgagcgta
    13141 aatttagcac ccggttaaac acaattaagc atagaggtta aaggagttaa ttatggatgt
    13201 taccgcaaaa tacgaattga ttgggctgat ggcctatcct atccgccaca gtttatcgcc
    13261 tgaaatgcag aataaagcct tagaaaaagc gggattgcca tttacctata tggcctttga
    13321 agtggataac gatagttttc ccggagcaat tgaaggatta aaagctctaa aaatgcgcgg
    13381 aactggtgta tcaatgccga acaaacaact ggcttgttaa tatgttgatg aattaacgcc
    13441 agcagccaaa ctggtgggtg ccatcaacac catcgttaat gatgatggct acctgcgcgg
    13501 ctataacacc gacggcacgg gccatattcg cgccattaaa gagagcggtt ttgatatcaa
    13561 aggcaaaaca atggtgctgt taggggccgg aggtgcctca acggctattg gcgcgcaggg
    13621 ggcaattgaa ggtttaaaag aaattaaact ctttaaccgt cgggatgagt tcttcgataa
    13681 agccctcgcc ttcgcgcagc gggttaacga aaacaccgat tgtgtcgtca cggttaccga
    13741 tctcgccgat cagcaagcct ttgctgaagc cctggcttcc gccgacattt taaccaatgg
    13801 cacaaaagtg ggtatgaaac cccttgagaa tgaatcatta gttaatgata tcagtctgtt
    13861 acatccggga cttctggtca ctgaatgcgt gtataacccg catatgacga agttattgca
    13921 gcaggcgcaa caagctggtt gcaaaacgat tgatggatac ggcatgttgt tgtggcaagg
    13981 ggctgaacag ttcacgttat ggactggcaa agatttccct ctggaatatg ttaaacaggt
    14041 catggggttc ggtgcctgac aggctgaccg cgtgcagaaa gggtaaaaaa atgaaaaccg
    14101 taactgtaaa agatctcgtc attggtacgg gcgcacctaa aatcatcgtc tcgctgatgg
    14161 cgaaagatat cgcccgcgtg aaatccgaag ctctcgccta tcgtgaagcg gactttgata
    14221 ttctggaatg gcgtgtggac cactttgccg acctctccaa tgtggagtct gtcatggcgg
    14281 cggcaaaaat tctccgcgaa accatgccag aaaaaccgct gctgtttacc ttccgcagtg
    14341 ccaaagaagg cggcgagcag gcgatttcca ccgaggctta tattgctctc aatcgtgcag
    14401 ccatcgacag cggcctggtt gatatgatag atctggagtt atttaccggc gatgatcagg
    14461 ttaaagaaac cgtcgcctgc gcccacgcgc atgatgtgaa agttgtcatg tccaaccatg
    14521 acttccataa aacgccggaa gccgaagaaa tcattgcccg tctgcgcaaa atgcagtcct
    14581 tcgacgccga tattcctaag attgcgctga tgccgcaaag taccagcgat gtgctgacgt
    14641 tgcttgccgc gaccctggag atgcaggagc agtatgccga tcgtccaatc atcacaatgt
    14701 caatggcaaa aactggcgta atttctcgtc tggctggtga agtatttggc tcggcggcaa
    14761 cttttggtgc ggtaaaaaaa gcctctgcgc cagggcaaat ctcggtaaat gatttacgca
    14821 cagtattaac tattttacat caggcataag caataatatt tcggcgggaa tatcctcccc
    14881 cgccgaacta aaaaaaatat attcaaccgt atttaataaa aatatttcgt gagtctctgt
    14941 gcgctaaata tccatctggc gtagggaaaa tcacacctga atcagaaatt aacaatgaaa
    15001 cctgtaaaac cacctcgtat taatggacga gtgccggtcc tgtcggcaca ggaagcggtg
    15061 aattatattc ccgacgaagc aacactttgt gtgttaggcg ctggcggcgg tattctggaa
    15121 gccacgacgt taattactgc tcttgctgat aaatataaac agactcaaac gccacgtaat
    15181 ttatcgatta ttagtccaac agggcttggc gatcgcgccg accgtggtat tagtccgctg
    15241 gcgcaagaag gtctggtgaa atgggcatta tgtggtcact ggggacaatc gccgcgtatt
    15301 tctgaactcg cagaacaaaa taaaattatt gcttataact atccacaagg tgtacttaca
    15361 caaaccttac gcgccgccgc agcccaccag cctggtatta ttagtgatat tggcatcggg
    15421 acatttgtcg atccacgcca gcaaggcggc aaactgaatg aagtcacgaa agaagacctg
    15481 attaaactgg tcgagtttga taacaaagaa tatctctatt acaaagcgat tgcgccagat
    15541 atcgccttca ttcgcgctac cacctgcgac agtgaaggct acgccacttt tgaagatgag
    15601 gtgatgtatc tcgacgcatt ggttattgcc caggcggtgc acaataacgg cggtattgtg
    15661 atgatgcagg tgcagaaaat ggttaagaaa gccacgctgc atcctaaatc tgtccgtatt
    15721 ccgggttatc tggtggatat tgtggtggtc gatccggatc aaagccaact gtatggcggt
    15781 gcgccggtta accgctttat ttctggtgac ttcacccttg atgacagcac caaacttagc
    15841 ctgcccctaa accaacgtaa attagttgcg aggcgtgcat tattcgaaat gcgtaaaggc
    15901 gcggtgggga atgtcggcgt cggtattgct gacggtattg gcctggtcgc ccgggaagaa
    15961 ggttgtgctg atgactttat tctgacggta gaaacaggtc cgattggcgg aattacttca
    16021 caggggatcg cctttggcgc gaacgtgaat acccgtgcca ttctggatat gacgtcccag
    16081 tttaattttt atcacggtgg tggtctggat gtttgttatt tgagttttgc tgaagtcgac
    16141 cagcacggta acgtcggcgt gcataaattc aatggcaaaa tcatgggtac cggtggattt
    16201 attgatatca gtgccacctc gaagaaaatt attttctgcg gcacgttaac cgcgggcagt
    16261 ttaaaaacag aaattgccga tggcaaatta aatatcgtcc aggaaggacg ggtgaagaaa
    16321 tttattcagg aactaccgga aattactttt agcggaaaaa tcgcgctcga acgggaactg
    16381 gatgttcgtt acatcaccga acgtgctgta ttcacgctga aagaagacgg cctgcattta
    16441 atcgaaatcg cccctggcgt cgatttacaa aaagatattc tcgacaaaat ggatttcacc
    16501 ccagtgattt cgccagaact caaactgatg gacgaaagat tatttatcga tgcggcgatg
    16561 ggttttgtcc tgcctgaagc ggctcattaa caggagtatc acgatggatt tttctttaac
    16621 tgaagaacaa gaactgctgc tggccagtat tcgcgaactg attacgacta actttgcgga
    16681 agagtatttc cgcacctgcg atcaaaacgg gacatatccg cgtgagttta tgcgggcgct
    16741 ggcggataac ggcatttcta tgcttggcgt gccggaagag ttcggtggca tccctgcgga
    16801 ttacgtcacc cagatgctgg cgctgatgga agtgtcaaaa tgcggtgctc cggcattttt
    16861 gattaccaac ggtcaatgta ttcacagtat gcgccgtttc ggttctgcag agcagctacg
    16921 taaaacggca gagagcaccc tggaaacggg tgatcccgcc tatgccctgg cgttgacgga
    16981 gccaggcgca ggctcagaca acaacagtgc cactaccact tacacgcgta aaaacggcaa
    17041 ggtttacatc aacggacaga aaacctttat taccggtgcg aaagagtacc cgtatatgct
    17101 ggtgttggcg cgcgatccgc aaccgaaaga tcccaaaaaa gccttcaccc tgtggtgggt
    17161 cgactccagt aagcccggca ttaagattaa cccgctgcat aaaatcggct ggcatatgct
    17221 cagcacctgc gaagtctatc tcgacaacgt ggaggttgaa gagagcgaca tggtgggcga
    17281 agaaggaatg ggtttcctca atgtgatgta caactttgag atggagcgcc tgatcaacgc
    17341 cgcgcgcagc accggctttg ccgaatgcgc ctttgaagat gccgcccgct atgccaacca
    17401 acgtatcgct tttggtaagc ccattggtca taaccagatg atccaggaaa aactggcgct
    17461 gatggcgatt aagatcgaca acatgcgcaa catggtgctg aaagtggcat ggcaagccga
    17521 tcagcatcag tcactgcgca ccagcgcggc gctggcaaaa ctgtattgcg cacgtaccgc
    17581 aatggaagtc attgatgatg cgattcaaat catgggcggt ctgggctata ccgatgaagc
    17641 gcgcgtctcc cgcttctggc gtgatgtccg ttgtgaacgt atcggcggcg gtacagacga
    17701 aattatgatt tacgtagcag gtcggcagat cctgaaagac tatcagaaca aataatctgc
    17761 aggcggcgca gcttcttaac aaactgcgcc gccagattta tccaacaaga cttaccggtt
    17821 gaggaaattc agataggatt tcttgttccg caaaacagtt tttaaattgt ctacggtaat
    17881 cgctgggcga acaaccgaca tggcgcaaaa acagtttggc aaagtgatcg acattttcat
    17941 aacccacacg ccaggagatc tctgcctgtg ataattcagt attagtgagt gaccattttg
    18001 cttccgtcat acgccgttga ataacatagt taatagggga aatacgatac tctttggtaa
    18061 attcatggca aatataactg acgctggcac gaaatttttt cgataactgt tctaaagtga
    18121 ttttttcgcg ataattatta ctcagataga aaagaatatc ttttatcaga acatcttttt
    18181 taatataccc ctgctccgaa cgataagcat ttttaaaatt ttcgtagtaa agaatggcta
    18241 acgtataggc aaatgcatcc cataacgaag atgtttggct atttttactt tgcggcaaaa
    18301 tcacacttag ctcattaaat aaggttttta tgacttcttt tccctgcccc gcggcaatta
    18361 ccggacaaga atgcggttgc agtagctgat tttcctcagc cccctgaaac tgaaagccgt
    18421 acagcgcaca ggtacacgtc gttgccggat cgttaacgtc agaggccacc gcatgcagcc
    18481 tgccgcgttc tatcaccaca atgtcatccg catgtgcgac atataacgaa gaatcgatgg
    18541 taaaccttgc aacccctttc ttgacgtaaa tcaactcggt ttcattgtca tgaacgtgat
    18601 gtccggactc ccattttgga tcatcgctaa atgcaaaacg tgaaagccgt ggcgttttac
    18661 cggcgacaaa cagcgtttca ctggcgttat caaaacagcg ttgatacatg acaacctccc
    18721 tattccatga gcaagcaaaa acaatatatg ccggataaaa tacggcgtgt attgcggtta
    18781 tacaaccgcg tttagcgaga gctaaacaag attatttaca ctgaatgcaa gattgtacgg
    18841 tcatggaata actatattgc cgcaaattgc acttttgttt tagcgatcgc atttttttgc
    18901 aagattgttg gcaaggaaaa cagcttgctc cgtcgaaaac ccccaccgct atcgcacact
    18961 attttcaggc catttttacc ttccatcgga gatggttccg tatgcgactc acaggagaaa
    19021 tcatgaaaat aataacctgc tttaagctgg tgcctgaaga acaggacatt gttgtcactc
    19081 cagaatacac cctgaatttc gacaatgccg acgccaaaat cagccagttc gatctcaatg
    19141 ccattgaagc tgcaagccag ctcgcaacag atgacgatga gatagccgcg ctgaccgttg
    19201 gcggctcatt gttgcagaac tcgaaagtgc gcagtgcgca aagatgtgct atcccgcggg
    19261 ccgcacagcc tgtatttggt gcaggatgcg caacttgaac atgcactgcc tctcgatacc
    19321 gcaaaagcgc tggcggcagc aattgaaaag atcggcttcg atttactgat ctttggtgaa
    19381 ggttccggcg acctttatgc ccagcaggtt ggcttgctgg tcggagaaat tctgcaactt
    19441 ccggtgatta atgcagtgag tgctattcag cgtcagggta atacactggt gattgaacgc
    19501 acgcttgaag atgatgttga agttattgaa ctctctgttc cagccgtgct ctgcgtcacc
    19561 tccgatatta acgtgccacg cattccttcg atgaaagcca ttctcggcgc gggtaaaaaa
    19621 ccggtaaatc agtggcaggc aagtgatatt gactggagcc agagcgcgcc acttgctgaa
    19681 ctggtcggca ttcgcgtacc gccgcaaaca gaacgtaagc acatcattat cgataacgat
    19741 tcgccggagg ccattgccga gctggcggaa catctgaaga aagccctgaa ctgaagccta
    19801 cggagaagaa acgatgagtc aattaaacag cgtctgggtc tttagcgata atcctgaacg
    19861 ttatgctgaa ctgtttggcg gcgctcagca atggggccaa caggtgtatg ccattgtaca
    19921 aaataccgac caggcgcagg cagttatgcc ttatggtcca aaatgtattt atgttcttga
    19981 gcaaaacgac gcgctgcaac gcactgaaaa ttacgccgaa agcattgctg ccccgctgaa
    20041 agataaacac cccgctatgt tgctgttggt cgcgacgaaa cgtggtaaag ctctggcagc
    20101 acggttaagt gtgcaactga atgcggcgct ggtgaacgat gccacggcgg tggatattgt
    20161 cgatggtcac atttgcgccg aacaccggat gtatggcggg ttagcgttcg ctcaggaaaa
    20221 gatcaacagc ccgctggcga tcattaccct tgcacccggt gttcaggaac cgtgcaccag
    20281 tgatacctct catcagtgcc cgacagaaac ggtaccttat gttgctccgc gtcatgaaat
    20341 tctctgtcgc gaacgccgtg cgaaagccgc aagcagcgtg gacctgagca aagcaaaacg
    20401 tgtggttggc gtcggtcgtg gtctggcggc gcaggatgac ctgaaaatgg tccacgaact
    20461 ggcggcggtg ctgaatgctg aagtcggctg ttcacgtcca attgccgaag gcgagaactg
    20521 gatggagcgt gaacgttata tcggtgtctc cggcgtgttg ctgaaatccg atctctacct
    20581 gacgctgggg atctccgggc agatccagca tatggttggc ggcaacggcg caaaagtgat
    20641 tgtcgccatc aataaagata aaaatgcgcc aatcttcaat tatgccgact acggtctggt
    20701 gggcgatatc tacaaagtcg tccctgccct gattagccag ttgagccgct aattcccctt
    20761 tcaaccacat ctcccgctgt aaacgcagcg ggaagggagc ataacgcatg tcggatgaca
    20821 aatttgatgc cattgtggtc ggtgcggacg ttgctagtag cgttgccgca ctggtcatgg
    20881 cgcgagccgg actggatgtc ctggtgatag aacgcggcga cagtgccgga tgtaaaaaca
    20941 tgaccggcgg gcgtctttat gcccacacac ttgaagcaat cattccaggc tttgcagtat
    21001 cagcgccggt agaacgcaag gtcacacgcg agaaaatctc cttcttaacc gaagaaagcg
    21061 ccgttaccct cgattttcac cgcgagcaac cagatgttcc gcaacacgca tcttataccg
    21121 tattgcgtaa tcgtctggac ccgtggttga tggaacaagc cgagcaggcg ggcgcacagt
    21181 ttatcccggg cgttcgcgtc gatgcgttgg ttcgtgaagg aaacaaggtc actggcgtgc
    21241 aggccgggga tgatattctc caagcgaatg tggtggttct agctgatggc gtaaactcga
    21301 tgcttggccg ctcgctggga atggttcccg cttccgatcc gcatcattac gctgttggtg
    21361 ttaaagaggt tattggcctc acaccagaac agatcaacga tcgctttaat attacgggcg
    21421 aggaaggtgc cgcctggctg tttgccggct ccccttctga cggcctgatg ggcgggggat
    21481 ttctctatac caacaaggat tccatatcct tggggctggt ttgtggattg ggtgatatcg
    21541 cccatgcgca aaaaagcgtg ccgcaaatgc tggaagattt taaacaacac cccgccattc
    21601 gcccactaat tagcggcggc aaactgcttg aatattccgc gcatatggtg ccagaaggcg
    21661 gtctggcaat ggtgccgcag atggttaacg atggcgtgat gatcgttggt gacgccgcag
    21721 gcttctgcct gaatttgggt tttacagttc gtggcatgga tttagccatt gcatcggctc
    21781 aggctgccgc cacaacggtg atcgccgcca aagaacgcga ggatttctcc gccagcagtc
    21841 tggcgcaata caaacgtgag ctggaacaaa gctgcgtcat gcgcgatatg cagcatttcc
    21901 gcaagatccc ggcgctgatg gaaaacccgc gcctgtttag ccaatactca cgaatggtcg
    21961 ccgacatcat gaacgagatg ttcaccattg acggcaaacc aaaccagccg gtacgcaaaa
    22021 tgatcatggg acacgcgaag aaaattgggc tgatcaactt gctgaaagat ggcattaagg
    22081 gagcaaccgc gctatgagcc agaacgctac ggttaacgtc gacatcaaat taggcgtcaa
    22141 taaattccat gttgatgagg gccacccgca tatcattctg gcggcaaatc ccgatatcaa
    22201 tgaattccgt aaattaatga aagcttgccc tgccggactt tataagcagg atgacgccgg
    22261 caacattcat ttcgattccg ccggttgtct ggagtgcggc acctgtcggg tgctgtgcgg
    22321 caacactatt ctcgaacagt ggcaatatcc cgcaggcacc ttcggtattg attttcgcta
    22381 cggctaatca tgcatcccac aggcccgcat ctcgggcctg atgttctgtt tcgagagtcc
    22441 aaaatgaaag tgacattaac gttcaacgaa caacgtcgtg cggcgtatcg tcagcaaggg
    22501 ttatggggcg atgcttcact ggccgattac tggcagcaga ccgctcgtgc gatgccagac
    22561 aaaattgccg tggtcgacaa tcatggtgca acgtacacct atagcgcgct cgatcacgcc
    22621 gcgagctgtc tggcaaactg gatgttagcg aagggtattg aatcaggcga tcgcatcgca
    22681 tttcaactgc ctggctggtg tgaatttacc gttatctatc ttgcctgcct gaaaatcggt
    22741 gcggtttccg tgccgctgtt gccttcctgg cgggaagcag aactggtgtc ggtgctcaat
    22801 aagtgtcagg caaaaatgtt ctttgcaccg acgttgttta aacaaacacg tccggtagat
    22861 ttaatcctgc cgctgcaaaa tcagcttcca caactacaac aaattgtcgg cgtggacaaa
    22921 ctggctcccg ccacctcttc cctctcatta agtccgatta tcgccgacaa tacctcactg
    22981 accacggcga taacgaccca cggcgatgaa ttagctgcgg tgctgtttac ctccggaacc
    23041 gagggtctgc caaagggcgt gatgctaacg cataacaata ttctcgccag tgagcgggct
    23101 tattgcgcgc gactgaatct gacctggcag gatgtcttta tgatgcctgc gccacttggt
    23161 cacgcaacgg gctttctgca tggcgtaacg gcaccattct taattggcgc tcgcagcgtg
    23221 ttgttagata ttttcactcc tgatgcgtgt ctcgcgctgc ttgagcagca gcgttgcacc
    23281 tgtatgctag gcgcaacgcc gtttgtctat gatcttttga atgtactaga gaaacaaccc
    23341 gcggaccttt cagcgctgcg tttctttctt tgcggcggta ccacaatccc caaaaaagtg
    23401 gcacgtgaat gccagcagcg cggcattaaa ttattaagtg tttatggttc cacagaaagt
    23461 tcgccgcatg cggtggtgaa tctcgatgat cctttgtcgc gctttatgca caccgatggt
    23521 tacgctgccg caggtgtaga gattaaagtg gtcgatgacg cacgcaagac cttaccgcca
    23581 ggttgcgaag gtgaagaagc ctcgcgtggc cccaatgtgt ttatggggta ttttgatgaa
    23641 cctgaattaa ccgcccgtgc gctggacgaa gaaggctggt attgcagcgg cgatctctgc
    23701 cgtatggatg aggctggcta tataaaaatt accggacgca aaaaagatat tattgtccgc
    23761 ggcggcgaaa atattagcag ccgtgaagtg gaagatattt tattgcagca tcctaaaatt
    23821 cacgatgcct gtgtggttgc aatgcccgat gaacgtttag gtgaacgatc atgcgcttat
    23881 gtcgtgctga aagcgccgca tcattcatta tcgctggaa
//
LOCUS       DAJTOR010000049        23493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTOR010000049 DAJTOR010000000
VERSION     DAJTOR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.049"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(297..611)
                     /gene="pspE"
                     /locus_tag="OO249_001739"
     CDS             complement(297..611)
                     /gene="pspE"
                     /locus_tag="OO249_001739"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5636072.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(686..907)
                     /gene="pspD"
                     /locus_tag="OO249_001740"
     CDS             complement(686..907)
                     /gene="pspD"
                     /locus_tag="OO249_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5636073.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(916..1275)
                     /gene="pspC"
                     /locus_tag="OO249_001741"
     CDS             complement(916..1275)
                     /gene="pspC"
                     /locus_tag="OO249_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5636074.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1275..1499)
                     /gene="pspB"
                     /locus_tag="OO249_001742"
     CDS             complement(1275..1499)
                     /gene="pspB"
                     /locus_tag="OO249_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5636075.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1553..2221)
                     /gene="pspA"
                     /locus_tag="OO249_001743"
     CDS             complement(1553..2221)
                     /gene="pspA"
                     /locus_tag="OO249_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5636076.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKESGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2388..3365
                     /gene="pspF"
                     /locus_tag="OO249_001744"
     CDS             2388..3365
                     /gene="pspF"
                     /locus_tag="OO249_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5636077.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3485..4750)
                     /gene="puuE"
                     /locus_tag="OO249_001745"
     CDS             complement(3485..4750)
                     /gene="puuE"
                     /locus_tag="OO249_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5636078.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4788..6057)
                     /gene="puuB"
                     /locus_tag="OO249_001746"
                     /pseudo
     CDS             complement(4788..6057)
                     /gene="puuB"
                     /locus_tag="OO249_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
     gene            complement(6059..7546)
                     /gene="puuC"
                     /locus_tag="OO249_001747"
     CDS             complement(6059..7546)
                     /gene="puuC"
                     /locus_tag="OO249_001747"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5636079.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FSDCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWMRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7821..8378)
                     /gene="puuR"
                     /locus_tag="OO249_001748"
     CDS             complement(7821..8378)
                     /gene="puuR"
                     /locus_tag="OO249_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5636080.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8405..9168)
                     /gene="puuD"
                     /locus_tag="OO249_001749"
                     /pseudo
     CDS             complement(8405..9168)
                     /gene="puuD"
                     /locus_tag="OO249_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            9380..10798
                     /gene="puuA"
                     /locus_tag="OO249_001750"
     CDS             9380..10798
                     /gene="puuA"
                     /locus_tag="OO249_001750"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5636081.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10947..11112
                     /locus_tag="OO249_001751"
                     /pseudo
     CDS             10947..11112
                     /locus_tag="OO249_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11102..12487
                     /gene="puuP"
                     /locus_tag="OO249_001752"
     CDS             11102..12487
                     /gene="puuP"
                     /locus_tag="OO249_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5636082.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12621..12866
                     /locus_tag="OO249_001753"
     CDS             12621..12866
                     /locus_tag="OO249_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5636083.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13179..14822
                     /gene="sapA"
                     /locus_tag="OO249_001754"
     CDS             13179..14822
                     /gene="sapA"
                     /locus_tag="OO249_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5636084.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLKFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14819..15784
                     /gene="sapB"
                     /locus_tag="OO249_001755"
     CDS             14819..15784
                     /gene="sapB"
                     /locus_tag="OO249_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5636085.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15771..16661
                     /gene="sapC"
                     /locus_tag="OO249_001756"
     CDS             15771..16661
                     /gene="sapC"
                     /locus_tag="OO249_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5636086.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMLAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16661..17653
                     /gene="sapD"
                     /locus_tag="OO249_001757"
     CDS             16661..17653
                     /gene="sapD"
                     /locus_tag="OO249_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5636087.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17655..18461
                     /gene="sapF"
                     /locus_tag="OO249_001758"
     CDS             17655..18461
                     /gene="sapF"
                     /locus_tag="OO249_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5636088.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18529..18882
                     /locus_tag="OO249_001759"
     CDS             18529..18882
                     /locus_tag="OO249_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5636089.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19250..20038
                     /gene="fabI"
                     /locus_tag="OO249_001760"
     CDS             19250..20038
                     /gene="fabI"
                     /locus_tag="OO249_001760"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5636090.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20183..21310
                     /locus_tag="OO249_001761"
     CDS             20183..21310
                     /locus_tag="OO249_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5636091.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTARDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     TAQFSPLLEHEQSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21378..23312
                     /gene="rnb"
                     /locus_tag="OO249_001762"
     CDS             21378..23312
                     /gene="rnb"
                     /locus_tag="OO249_001762"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5636092.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23390..23493)
                     /locus_tag="OO249_001763"
                     /pseudo
     CDS             complement(23390..23493)
                     /locus_tag="OO249_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5311 a         6210 c         6114 g         5858 t
ORIGIN      
        1 gacgcgttac aagtttctgt aaatgagttg cggtaaatgt tccaccgtag atttcgtcca
       61 ggtaatcagt aattttctgt ttcatgttgc ctttcagtgg tagcgatccc atgaaacgag
      121 agactactcc gcagaaaata aactgtcaat tgagcacaag ggttttagtc acgcaggaaa
      181 tcatttttta ccagggaaaa agcgtatttg tgagctgacg caaataatct atggggatgt
      241 aaataaggtg tgacagaaaa aaaacggcgc ataagcgccg ctcatggtga attcttttaa
      301 cctttgacct tcggcattgc gatgtctttc aagccaccgg cgttctcaac gtgggtatat
      361 cccatctcgc taaggatctc ttttgcttgc cctgactggc gtccggcatt gcaatacact
      421 ttcacggtgt cgtttttatc cggaacggcg gtggcaatgc gctctttcac ttccttcagg
      481 ggaatattga tggccccctg aacgtgctct tgctgatact gctctggaac acgaacatcg
      541 atccagtgtt cagcggcgaa aacgggcagt gaaaacacca gtgccagagc aagtaagcct
      601 tttttaaaca tgtaagcgtc cttctatgga tgaaaattgc catccattat acgcctgaga
      661 tttaacaatt tttgcgaaac tcccctcacc ttttataacg ctgtgccagt ttattagcag
      721 cccgacttaa cagcggttcc agcgccacag ccagcaacat tttcagcggt cggcgagcaa
      781 ctgattttat cgcccagccc gctacacccg ccgggccata gcgcagtgcg gtaagaagta
      841 ccagcttgcc tgctaatttg aaaccaggct ttaccttttg cccggcctgt tgccagcgag
      901 tattcataac tttcctcaca gttgacggaa acggctacgt aacgtgaaag tatcggaagt
      961 gacataacgt tccatctcgc gtaaacgcgt ttcacttgcc gccagttcac ggtcgacttc
     1021 atccagcaat tcgctgctgg agggtagctg ctcaccaaag gccatgttgt ccggcattgg
     1081 atcaagcgca aatgacaaaa tgatgtaagc aaccagggta aacagcgcca gaccgaagaa
     1141 aatcgacagc accaccagga tacgcaccag ttttaccggt acatcaaaat agttggcaat
     1201 cccggcgcag acgccgcgga ccatgccctg ctgtggaata cgccataatt ttttattgag
     1261 attaatgccc gccattagcg atccctccag ttcggatgtt ctgcatcaag aatagattcc
     1321 agcgcctgaa tacgttcgcg catccgtttt gcttcatcag ccagttgcgc taatcgctgc
     1381 tgctcacttt gcgacaattc actgcgacca gaacgattgc tgtaatgcag ccataaccag
     1441 atcggtaaaa caaacagcac aaaaatggtt aacggaatag ccagaaatag cgcgctcata
     1501 agtactcctt agacgatgag cggcggcgcg ttcggacgcc gcctagatgt tattattgat
     1561 tgtcttgctt cattttggct tttaattgtg ccagttgttc gctgattgca tcatcggctt
     1621 tcagttcggc aaactgatcg tccagcgatt tttgtttacc gaagctgtgg ctttctgctt
     1681 ccgcttccat ctggtcaata cgacgttcga aagattcgaa acgagccatt gcttcatcca
     1741 gtttgccact gtccagctga cgacgcacat cgcgcgacga gtttgccgcc tgatggcgta
     1801 acatcaatgc ctgctggcga gcgcgtgttt cgctcaattt gttttccagc tcaccgctct
     1861 ctttcttcat gcgtgccagc gtatcgtcca ccagcgtcac ttcatgttcc agactcttaa
     1921 tcagatcggt cagtttctgt ttttcaatta acgccgcacg tgccaaatcc tctctctctt
     1981 tcagcagcgc cagttcggct ttttcctgcc attcaacttc acgcgccgac gcttgttcaa
     2041 tacggcgagt cagctgtttc ttttctgcca acgcacgcgc cgaagtagaa cgtacttcaa
     2101 ccagtgtatc ttccatctcc tggatcatca gacgcaccag tttctgtgga tcttccgctt
     2161 tctctaacag agcgttgatg ttggcattca cgatgtcggc aaagcgagaa aaaataccca
     2221 taatgttgtc ctcttgattt ctgcgttcag gattgtcctg ctgaactgtt aatacaattt
     2281 gcgtgccaat tttttatctt tttgatttat aaagatctga ttgaagaatc atcagcaaca
     2341 tgccaggatg aattagcgaa ttacactaac aagtggcgaa tttcatcatg gcagaataca
     2401 aagataattt acttggtgag gcgaacagct ttctcgaagt gctggaacag gtttcgcatc
     2461 tcgcaccgct ggacaaaccg gtgctcatca tcggcgaacg cggcaccggt aaagagctga
     2521 ttgccagccg cctgcattat ctctcctccc gttggcaagg gccgtttatt tcccttaact
     2581 gcgcggcgtt aaatgaaaat ctgctggatt ccgaactgtt tggtcacgaa gcgggggcgt
     2641 ttaccggtgc gcaaaaacgt catccaggga gatttgaacg tgccgacggc ggtacgctat
     2701 ttcttgatga actcgctacg gcaccgatga tggtgcagga gaaattattg cgcgtgattg
     2761 agtacggtga actggagcgc gttggcggca gccagccatt gcaggtgaat gtgcggttgg
     2821 tatgcgcgac gaatgccgat ctcccggcga cggtcaatga aggcactttt cgcgctgacc
     2881 tgctcgaccg actggctttt gatgttgtac aactgccacc actgcgcgag cgcgaaagcg
     2941 acataatgtt gatggcagaa cactttgcca tccagatgtg tcgggaaatc aagctgcctc
     3001 tgttcccggg gtttacggag cgcgccagag aaacattgct gaattatcgt tggccgggaa
     3061 atattcgtga attgaaaaac gtggtggaac gttcagtgta tcgccacggc accagcgatt
     3121 atccgcttga tgacatcatt attgatccct ttaaacggcg tccgcctgaa gacgctatcg
     3181 ccgtttcaga aaccacctcg cttccaacac tgccgctgga tttacgtgag tttcagatgc
     3241 agcaggaaaa agagttgctg caactcagtt tgcaacaagg gaaatataac cagaagcgcg
     3301 cggctgaatt actggggtta acttatcatc agttccgcgc gttgttgaaa aagcaccaga
     3361 tttagcgaag ttgagcaata caacttgccg gatgcggcgt gaatgcttta ttcggcatac
     3421 ataatcaata agtcgtttgc aggcctgata agcgcagcgc atcagacatt attgcgttgg
     3481 gctattaatc gctcagcgca tcctgcaaaa ttttcattgc cgcatcgaat tgcgcatccg
     3541 gaatggtcag cggatacagg aagcgaatca cgttgccgta tgcgccacag gtcagcagga
     3601 gcagcccctg cgccagcgcg cgttgctgga ttttctgtgc aatcgccgct gacggctcgc
     3661 ccgtttgcgg atcgttaaac tctgccgcaa tcatcgaccc caggccgcgt accgcagcaa
     3721 tggccggaac gctttctttg gcatcaatca acgtgttttt gagacgctgg cccagttgat
     3781 tcgcgcgttc gcagagtgat tctttgtcga taatattgag caccgcgtgc gcggcagcca
     3841 ccgccagcgg gttcccggcg taggtgccac ccaacccgcc cggcgcgggc gcgtccataa
     3901 tattcgcgtt accgaccacg cccgaaagcg gcatcccgcc cgcgaggctt ttcgccatcg
     3961 tcattaagtc cggcttatcg gcgtaatgat ccatggcaaa cagtttaccg gtacgcgcaa
     4021 agccgctttg cacttcatcg gcaatcatca caataccgtg ctcgtcgcac aggcggcgaa
     4081 tggcggcaac cagctctttt ggcgcaacgt tgaaaccgcc ctcgccctgc accggttcga
     4141 aaataatggc cgccacctgc ttcgcttcaa tgtccgattt aaacaagcgt tcgatggcgt
     4201 cgagggagtc ctgtgttgaa atgccgtgta aatctgacgg ataaggtacg tgatacaccg
     4261 aaccagggaa cgggccgaag ccgattttgt acggcgcaac ttttccggtc agcgccatgg
     4321 tcatatatgt acgaccgtgg aagccgccgc taaacgcaat cacgccaggt cgtccggtat
     4381 gggcgcgagc aattttcacc gcgttttcca ccgcttccgc gccggtagtg aagaatgcgg
     4441 ttttagcctg cccgctcact ggggcaaggg cgttaatttt ctccgccagc gtgacgtagc
     4501 tttcgtacgg cacaatctga tacgccgtgt gggtaaactg ttgcaattgc tgctccaccg
     4561 ccgcgaccag atcagggtgg cgatgcccgg tattcagcac cgcaatgcct gcggcgaaat
     4621 cgatgtattc gttgccctct acgtccttca gcgtagcgtt ttctgccgac tgggcgaaga
     4681 agttacacat cacgccaacc ccgcgtgggg tagcagaaag acgacgctga tggaattcat
     4741 tgttgctcat gtttcacacc ttttgactct tcactgttaa tcggaaatta aacgcccaga
     4801 cgatcgcgca ggctgtaata agccgcgccc atcgcggtaa atggcacgcg cagcgtgcgc
     4861 ccgccgggga acgggtaatg cggcagattg gcgaaggcat cgaaacgctc ggcgtcaccg
     4921 cgcagcagtt cagcaatcaa acgcccggcg aggtgggtac aggtgacgcc gtggccgctg
     4981 tagccctgca tgtaatagat gttggtatcg aggcgaccga actgcggcat ccgtgaaagg
     5041 gtcagcagga agttgcccgt ccagcgatag tcgattttga cgcccttcag ctgcgggaag
     5101 gttttcagca gtttcggcac aacaagacgc tcaacgtcat ccgggtcgcg cgcgccgtag
     5161 accacgccgc cgccgtacag caaacggttg tcggcggtaa gacggtagta atcaagcaga
     5221 taattacagt cttccacaca gtagtttttc gggatcagcg aacgggctaa atcttccgac
     5281 agccgttcgg tggtgatcac ctgcgtgccg cacggcatgc tgcgtttcgc cagttccggc
     5341 tctactttat cgcccagata cgcattcccg gcgacaatca catacttcgc cgtcacctga
     5401 cctttggcag tgcgcacaac ggctggcgtg gtgtgctgga tctgcgtcac ggcagaaagt
     5461 tcatacacgc gcccgccgtt gaggcggatg gcgtccgctt cgccaatggc gaggtttagc
     5521 gggtgaatat gcccaccgct gtgatccagc agcgcaccgg tatagcgatc gctggctact
     5581 tcacggcaaa tggcgttggc gtccagcaat tccagctgtt tattgccgta gcgttcccag
     5641 ttctctttct gctcttcaag tgtggcgagc tgtttatcat tcatcgccac aaacaggctg
     5701 ccggggcggt agtcgcagtc aatctgataa cgtttgatac gttcgcggat gatctcacca
     5761 ccttcgaaca tcatgctgcc gagcataggg gctgtgtcca tgccgtagct tttttcgatc
     5821 acgtcgatgt cgcggttgta ggagttcaca agctgcccgc cattgcgccc gcttgcgcca
     5881 aagccgatgc gtgaggcttc gagaaccact acgtcaaagc ccgcttccgc cagatgcagc
     5941 gcggaggaga gcccggtata gccgccgcca accacgcaaa cgtcgcaggt gattgactca
     6001 ttcagggtgt cgaatggcac gtatttatgc gtaataactg ctggtatgtt cggtcatttc
     6061 aggcctccag gcttatccag atggttttca gttcggtgaa tttttcaagg gcatgcaggg
     6121 atttgtcgcg accgttgccg ctctgcttat agccgccaaa cggcacggtc atatcgccgt
     6181 cgttgtagtt attgacgaag acggaaccgg ctttcaggcg gcggctcata cggtgcgcgc
     6241 gggagaggtc gcgcatccat accgccgcgc caaggccgta ctggctgtcg ttggcaagct
     6301 gtagcgcctg ttcttctgat gtgaaacgcg tgaccaccag caccggaccg aaaatctctt
     6361 cgcggcttaa ggacgcattc gggtctacat ccacaaagat ggtcgggccg atggcggcag
     6421 ccagcccggc gttacggcca tccagcagca gttgcccttt gccttcgccc tcacgaataa
     6481 agctatggac cgagtcggcg tgggcgcagt cgattaaagt gcccatggtg gttgcggggt
     6541 caagcggatg gcccggctgc cagttttgcg cctgctgttt taacagggct aagaattcat
     6601 cggcgatgct ctcttccagc aacaggcgtg ttccggcgat gcacacctga ccctggttgt
     6661 agaagatgcc tgctgcggtg gagctcgccg ctttttgcag atccgaacaa tcggagaaaa
     6721 cgatgttggc gcttttgccg cccgcttcca gccagacgcg tttcatgttg ctgtcgcccg
     6781 catctttcag cagctgtttc ccggtacggg ttgaaccggt aaaggcaatg gcgtcgatat
     6841 cgttatgacg cgacagcgcc tgccccgctt catgaccaaa acccgtcacc acgttcaaca
     6901 caccatccgg caagcctgct tctttcgcca gccccgcgag acgaatcgca ctgagcggtg
     6961 atttttcaga cggttttaga atcacgctgt ttcccgccgc cagcgccggg ccgagtttcc
     7021 agcaagtcag caacagcggg aagttccacg gcacgatggc ggcaatcacg ccgaccggtt
     7081 cacgcacgat catcgccagc tcatggctac tggtggtcgc cacttcgcca tacactttgt
     7141 cgatcgcttc ggcgtaccag cgaatggcgc gcgccgcgcc gggaatatca tcacgcagac
     7201 tgtgacgaat cggtttgccg gtgtcgagag tttccagcag tgccagctct tcggcgtggg
     7261 cttccattaa atcggcgagt ttattcagta ccgctttacg tttagccgga gaagagagtg
     7321 accagtcgcc gcgttcaaat acgccgcgtg ctgcgctcac cgcacggtcg atatcgacgc
     7381 tcttgccgcg ggcaattttc gccagcggtg cctgggtgac tggatcaacg gtttcaaagg
     7441 tttcattttc cgccgcagca gtatattcac cgttaataaa taagcggttt tcaatggcga
     7501 gacttaacgc tttatcctgc cagtaagcca gatgatgaaa attcattatg actcctgttt
     7561 cacgtctatc agatatatgc agtttgtgaa cgtcagggcg cagagatccc cgggccgtat
     7621 gcccgtttat ttctggcgac attctttata tacacttcat ccttcaagcc gcctctgcct
     7681 tggctgcgtt cgctcccccg aatcacttac ctgagtaagc tcatcgggat tcgctcactt
     7741 gccgccttga tgcaacttga atgattttgt gtatagattg ccgggcgcat tgcgcttacc
     7801 cggcttacag aacaaaaaga ttaaaacgtg gtgggcgtat gggcgctgat aattcggcaa
     7861 atacctgccg acgtattact gaaactgtgc gggatgccgg tattaatggc ataactttga
     7921 cccgcgacga ggtggtaatc ctgaccatta atcgtcagaa caatttcacc ttccagtact
     7981 gtgcctattt cctcaccctg atgcttaatt ctttccccag tggttgtgcc cggctggtac
     8041 gtttcaaaga tcatcgccag cgtgcgattc gggttaccgt tatgaaccag cttcattgac
     8101 acaccctgac tgcccatctc aattaagtcg tcctgattaa tgacgacctg cggctcatca
     8161 ggtttttccg gctcggaaaa gaattccgag agtgacagac catacacctt cagcagcttt
     8221 tgcagcgtac tgatggcagg gctgacttta tcttgttcta tcgtactgat agcactgtga
     8281 gtcagcccgg agagttcggc ggcacgacgt tgtgaaagcc cctgctgctg gcggatttcc
     8341 gacaagcgtt ttcctggcgc cagtccctca tcactcatat ttgcatttcc ttaaactgta
     8401 gtggtcagag tcgctgtttt tcagcgatat ggtgctgaca agcggtgata aagccctcga
     8461 acaatatacg cgaaagagcg tactcgctac tgttccattc cgggtgccac tgtacgccca
     8521 gcgcaaaagg atgattgatg acgctaaccg cctcaactaa gccatccggc gagcgagctt
     8581 caacacgcaa ccgtgggcta acgaccttcg ccccttgtcc atgtagagag tttacccaaa
     8641 agttgctaca ttcaggtaac aacgcagaca gtaatccccc ctcttcaacc tgaacttcat
     8701 gcgacggtgc atattgctgt tccaccggca gttcggatct tcccgatgtt ccagcaattc
     8761 aggctgttcg cacagcttgc gatgcaacga accaccggtt gccaccacca gttcttgtaa
     8821 accccggcag atggcgaaaa tggggatgcg cctttcgagt gcggcattaa ttatcgccat
     8881 gctcagaaga tcacgcccgg gatcggcgtc aggctcatcg ccgttttcac catataggtg
     8941 cggctgcaca ttgctgggac taccaggaag ataaatgcca tcgagtttcg gcaaaagttg
     9001 ttcaagtaac gacggttccg ccagcgcatg tggtagcgca attggcaagc cgcctgcgtg
     9061 gatgatggca ttcaggtact tttcttgcag agtctgggtc gcatgaccct taagcctgtt
     9121 cctgcacatt acgacaccga taaccggatt gttcattata tttttcatgc tcaatctcac
     9181 aaagtggact aaattatcgc cattactgcg ctttaaatga cctttatgtt caatattttt
     9241 tcaatctagc agtgggtttt catttttgca aactaaattt aacatttagc aaacatttag
     9301 tttgcataca gattcgaatg gtggtcatta tattttacgc tttgataacg agcggaaaac
     9361 aaaccaaagg cgaagaatca tggaaaccaa tatcgttgaa gtagagaact ttgttcagca
     9421 gccagaagag aggcggggta gcgcctttac gcaggaagtg aaacgctacc tggagcgcta
     9481 cccgaatacg caatatgttg atgttttact tactgatttg aatggttgct tccgtggaaa
     9541 acgtatccct gtttcaagcc tgaagaagtt ggagaaaggg tgttatttcc cggcctcggt
     9601 gtttgcaatg gatattctgg gcaacgtagt agaagaagcg ggtctgggtc aggaaatggg
     9661 cgagccggat cgtacttgtg ttcctgttct cggttcctta accccttctg ccgccgatcc
     9721 agagtttatc ggtcagatgc tcctgaccat ggtcgatgaa gatggcgctc cctttgacgt
     9781 tgagccgcgg aacgttctta accgcctgtg gcagcagcta cgccagcgcg gattgttccc
     9841 ggttgtagcg gtagagctgg agttctattt actggatcgt cagcgcgacg ctgaagggta
     9901 tctgcaaccg ccctgcgcgc ccggcaccga taaccgcaat acacaaagcc aggtttactc
     9961 cgttgataac ctcaaccact tcgccgacgt gctcaatgat attgatgaac tggcgcagtt
    10021 acagttaatt cccgccgacg gtgcggtcgc cgaagcctcg ccgggtcagt ttgaaatcaa
    10081 cctttaccat accgataacg tgctggaagc ctgcgatgat gcgctggcgc taaaactcct
    10141 ggtgcgtctg atggcagaaa agcataagat gcactccact tttatggcga agccgtatga
    10201 agagcacgcg ggcagcggga tgcatatcca tatcagtatg caaaataatc gtggcgagaa
    10261 tgtgctttct gacacagaag gcgaagattc gccgctgctg aaaaagatgc tcgccgggat
    10321 gattgacctg atgccgtcgt cgatggcgtt gctggcacca aacgtgaact cgtatcgccg
    10381 cttccagccg ggaatgtatg tgccgacgca ggcgtcgtgg ggccataaca accgcaccgt
    10441 cgccctgcgt attccgtgcg gcgaccgtca taatcaccgc gtggaatatc gcgtggcggg
    10501 tgccgatgcc aacccatatc tggtgatggc agcgattttt gccggtattt tgcatggcct
    10561 tgataacgag ctgccgttgc aggaagaagt cgaaggcaac gggctggaac aggaaggctt
    10621 acccttcccg attcgccaga gcgatgccct gggtgagttt atcgagaatg atcacctgcg
    10681 ccgctattta ggcgaacggt tttgccatgt gtatcacgcc tgtaaaaatg atgagctgtt
    10741 gcagtttgag cgcctcatca cagaaacaga aattgagtgg atgttgaaaa acgcatagta
    10801 ccccttgcgg ggttgtttca tcgacctgag cggagccagg cctgacattt gctggtaatc
    10861 cagacatttc ccggatgtcg cgttatggag cgcaggcggc aacgggcttc cgttaacgat
    10921 taagactgtg acgaggaaat gagatgatgc cgatgattaa atccccgcat ggcgagggag
    10981 gctgcgtatg cgcccctcct gctactgact ggactccgtc ccctttgctt cccttgctaa
    11041 acaggtttga tttcaggtcg acacgaccgc aaaccttatt acgcaggggg aggcagcaat
    11101 tatggctatt aattcaccac tgaatattgc tgcgcaaccc ggcaaaaccc gtctgcgaaa
    11161 atcactgaaa ttgtggcagg tggtgatgat gggtctggcc tatctcacgc cgatgaccgt
    11221 atttgatact tttggtattg tctccggcat tagcgacggt cacgttccgg cgtcctattt
    11281 gctggcgctg gcgggcgtgc tgtttaccgc tatcagctac ggcaaactgg ttcgccagtt
    11341 tccggaggcc ggttcggcct atacctacgc gcaaaagtcg attaacccgc acgtcggatt
    11401 tatggtcggc tggtcatcgc tgctggatta tctctttttg ccgatgatca acgtcttgtt
    11461 ggcgaaaatc tatctctccg ccctcttccc ggaagtgccg ccgtgggtgt gggtagtaac
    11521 cttcgtcgcc attttaaccg ccgcgaatct gaagagcatc aacctggtcg ctaacttcaa
    11581 taccctgttt gtactggtgc aaatctccat catggtggtg tttatcttcc tggtagttca
    11641 gggactgcat aaaggagaag gcgttggcac cgtctggtca cttcagccgt ttatcagcga
    11701 gaacgcgcac ctgatcccga ttattaccgg ggcgacgatt gtctgtttct ccttcctcgg
    11761 tttcgatgcg gtgaccacgc tttcggaaga gacgccggac gccgcacgcg tgatcccgaa
    11821 agccatcttc ctgacggcgg tctacggtgg cgttatcttt atcgcggcgt cgttctttat
    11881 gcagctgttc tttcccgata tcagccgctt taaagatccg gatgccgcac tgcctgaaat
    11941 tgcgctgtac gtcggcggca agctcttcca gtcgattttc ctctgcacca cgtttgtgaa
    12001 cacgttagcc tctggcctgg cttcacatgc cagcgtgtcg cgtctgctgt atgtgatggg
    12061 gcgtgacaat gtgtttccgg agcgcgtgtt tggctatgtg cacccaaaat ggcggactcc
    12121 ggcactcaac gtcattatgg tcgggattgt cgcgttgtcg gcgctgttct tcgatttagt
    12181 taccgccaca gcgttaatta acttcggtgc tctggtggcg tttaccttcg tgaatctgtc
    12241 ggtgtttaat catttctggc ggcgtaaagg aatgaataaa agctggaagg atcacttcca
    12301 ctatttgctg atgccgctgg ttggcgcgct gacggtgggt gtgctgtggg ttaacctcga
    12361 gtcaacgtca ctgacactcg gtctggtatg ggcttcgtta ggcggcgcat atttatggta
    12421 tctgatccgc cgctatcgca aagtgccgtt gtatgatggt gacagaacgc cggtgagtga
    12481 aacgtaatag tcggatgcgc ggcgcattat cctgccggag ggtaatgcac cgcgccatta
    12541 actataaaaa gatgtatgac ccggatcacg agtgctatca tccgccctta tgcttagcgc
    12601 attaacccca ggggcaaata atgaaccacg atatcccgct aaaatatttt gatattgccg
    12661 atgagtacgc gaccgaatgc gcagagcctg tcgcagacgc tgaacgcaca ccgctggccc
    12721 actacttcca gttactgctc acccgtttga tgaacaatga agagatcagc gaagaagccc
    12781 aacatgaaat ggctgctgaa gcggggatta atcccgtacg cattgatgag atcgcagagt
    12841 ttttgaatca atggggtaat gagtaattat ttaattgcag acgatcggtc agtatcgttg
    12901 tccggataaa gcgttcacgc cgcatccggc taacaatgac acgcactgca gcgtgcttta
    12961 atgggtttct cacgatcgta ccgctctgct cctcgctgag ttgttctgtg agttatagtt
    13021 atagaggaag cacgggtacg aatgttgaac agatggcgac gccacctgtt caacgaccaa
    13081 ccacacggcc ctcattacga cccctgaaca caaacggtaa tggcaaatgt cattaaatat
    13141 tagtaagata gcctctttac ataaaatccc ttaatattat gcgccaggta ttatcgtctc
    13201 ttttggtgat tgctggactt gtgagtggtc aggcaatcgc cgcgcctgaa tcccccccgc
    13261 atgctgatat ccgcgacagc ggttttgtct attgcgtcag cgggcaagtc aacaccttta
    13321 acccatccaa agcgagcagt gggttaattg tcgataccct tgccgcccag ttttatgatc
    13381 gactgctgga tgtcgatccc tatacctatc gcctgatgcc ggaacttgcc gaaagctggg
    13441 aagtactcga caacggcgcg acctatcgct tccacctgcg tcgcgatgtt ccgtttcaaa
    13501 aaaccgactg gtttactccc actcgtaaaa tgaatgccga cgatgtggtg tttaccttcc
    13561 agcgaatttt tgaccgcaac aacccgtggc ataacgtcaa cggcagcaac ttcccctact
    13621 tcgacagcct gaaatttgcc gataacgtga aaagcgtccg caaactggat aatcataccg
    13681 ttgagttccg actcgcccag ccggatgctt cttttttgtg gcacctcgca acccattatg
    13741 cttcggtcat gtcggcagaa tatgcccgga agttagagaa agaagatcgc caggagcaac
    13801 tcgaccgtca accggtcggc accggtccgt atcagttgtc ggaataccgc gccgggcaat
    13861 ttattcgcct acaacgtcat gatgacttct ggcgcggtaa accgttaatg ccgcaggtag
    13921 tggtggattt aggctccggc ggcaccggac gtctgtcgaa actcctgacc ggggaatgcg
    13981 acgttctggc ctggcctgct gccagccagc tatccatttt gcgtgacgac ccgcgcttgc
    14041 gtttaacgct gcgtcctggg atgaacgtcg cctatctggc atttaacacc gccaaaccgc
    14101 cgctaaataa tcccgctgtc cgccatgcac tggcactggc gattaataac cagcgcctga
    14161 tgcaatccat ctattatggt acggctgaaa cggcggcctc tattttaccg cgcgcctcgt
    14221 gggcctatga caacgaggct aaaattactg aatacaatcc ggcgaaatcg cgcgaacagt
    14281 tgaaggcgtt ggggctggaa aacttaacgc tgaaactgtg ggtgcccaca cgttcgcagg
    14341 cgtggaaccc cagtccactg aaaactgccg aactgattca ggcggatatg gcgcaggttg
    14401 gcgtaaaagt ggtgattgtg ccggtagaag gtcgctttca ggaggcacgg ttgatggata
    14461 tgagccatga tctgacgtta tccggttggg cgacggacag taacgacccg gacagtttct
    14521 tccgtccttt actgagctgc gcggcaattc attcacagac caacctcgcc cactggtgcg
    14581 atccgaaatt cgacagcgtg ttgcgtaagg cgctctcctc gcagcagctg gcggcgcgta
    14641 ttgaagccta tgacgaagcg cagagtattc tggcgcagga attgcccatt ttgccgctgg
    14701 cgtcgtcatt gcgtttgcag gcctatcggt acgatatcaa aggtctggta cttagcccgt
    14761 ttggtaacgc ctcctttgct ggggtgtatc gcgagaaaca ggatgaggtg aaaaaaccat
    14821 gattatcttc accttacgcc gcattttgtt attgattgtc accttgttcc tgctgacctt
    14881 cgttggcttc agcttgagct atttcacccc tcatgcgccg ttacaaggcg cgtcactgtg
    14941 gaatgcctgg gtgttctggt ttaacggcct gatccactgg gattttggtg tttccagcat
    15001 taacggtcaa ccgattgccg aacagttaaa agaggtcttc ccggcgacga tggaactgtg
    15061 catcctcgcc ttcggttttg cgctgattgt tggcatcccc gtggggatga ttgccgggat
    15121 tacgcgccat aagtggcagg ataacttgat caacgccatc gccttactgg gcttttcaat
    15181 cccggtgttc tggctggcgc ttctgttgac gctgttttgt tcactcacgc taggttggtt
    15241 gcccgtttca gggcgtttcg atctgctcta cgaagtgaaa ccgattaccg gttttgcgtt
    15301 gattgatgcc tggctttcgg actcaccatg gcgggatgaa atgatcatga gcgcaatccg
    15361 ccatatgata ttgcccgtga tcaccctgtc ggttgcgcca acaactgaag tgatccgcct
    15421 gatgcgtatc agcactatcg aagtgtatga ccagaactat gtaaaagcgg cggcgacccg
    15481 cggtttgtcg cgctttacta ttttgcgtcg ccacgttctg cataacgcgt taccgccggt
    15541 tattcctcgc ctgggcttac agttttccac catgctgacg ctggcgatga tcaccgaaat
    15601 ggtttttagc tggccgggtt tagggcgctg gttaattaac gccatccgcc agcaggatta
    15661 cgcagccatt tccgccggag tgatggtgtg tggctcactg gtcattattg ttaacgtgat
    15721 ttctgatatt ttgggtgcca tggctaaccc tctgaaacat aaggaatggt atgccttacg
    15781 atagcgtata cagcgaaaag cgcccgccgg gcacgctgcg taccgcctgg cgcaaatttt
    15841 atagtgacgc ctctgcgatg gtcggcctgt acggctgcgc ggggctggct gtactgtgta
    15901 tttttggcgg ctggtttgcg ccttacggca tcgaccagca atttctcggt tatcaattat
    15961 tgccgccatc atggtcgcgc tatggcgaag tttctttctt cctggggact gacgacctcg
    16021 gacgcgatgt tttaagccgt ttactgagcg gagctgcgcc aaccgtgggt ggcgcatttg
    16081 tggtgacgct tgccgcgacg atctgtggcc tagtactggg caccttcgcc ggggcgacac
    16141 acggcctccg ctcagcggtg cttaaccata ttctggatac cttactggca atcccttcgc
    16201 tgctgttggc aattatcgtt gtagcgtttg ccggaccgag tttgtcccac gctatgcttg
    16261 ctgtctggct ggcattgctg ccgcgtatgg tacgttcgat ttacagcatg gtgcatgacg
    16321 aactggaaaa agagtacgtt atcgccgccc gtctggatgg cgcatcaacg ctgaatattc
    16381 tctggtttgc tgtgatgcca aacatcaccg ccgggctggt caccgagatc acccgcgcac
    16441 tgtcgatggc aattctcgat atcgccgcgc tgggctttct cgatctcggc gcacaactcc
    16501 cctcgcctga atggggagcg atgctcggtg atgcgctgga actgatttat gtcgccccgt
    16561 ggactgtcat gctgccaggt gcggcaatta tgattagcgt gttgttggtt aacctccttg
    16621 gcgatggcgt tcgtcgtgcg attattgcgg gggtggaata atgccattac tcgatattcg
    16681 taacctgacc attgaattta aaaccggtga tgagtgggtt aaagccgtcg accgggtaag
    16741 tatgacctta accgaaggtg aaatccgcgg tcttgttggt gaatccggtt ccggcaagag
    16801 tttgattgcg aaagcgattt gtggggtgaa taaagataac tggcgtgtta ctgctgaccg
    16861 tatgcgtttt gatgatatcg atttgctgcg tctctccgca cgcgaacggc gcaaactggt
    16921 tggccataac gtgtcgatga ttttccagga accgcagtcg tgtcttgacc cttcagaacg
    16981 tgtgggtcgc cagttgatgc aaaacatccc agcctggacc tataaaggcc gttggtggca
    17041 gcgttttggc tggcgcaaac gccgtgccat tgaactgctg caccgcgtgg ggattaaaga
    17101 tcacaaagat gcgatgcgca gttttcccta tgagttgacc gaaggtgaat gtcagaaagt
    17161 gatgatagcc attgcactgg cgaatcaacc gcgtctgctg attgctgacg aaccgaccaa
    17221 ctcaatggag ccaacaaccc aggcgcaaat ctttcgcctg ctgacgcgtc tcaaccaaaa
    17281 cagcaatacc actattttgc ttatcagcca tgacttacaa atgcttagcc aatgggcgga
    17341 taaaattaac gtgctttact gcggtcaaac agtggaaacc gcgccgagta aggagttggt
    17401 gacgatgcca catcatcctt atacccaggc gctgatccgc gcgataccag acttcggcag
    17461 cgcgatgccg cataaaagtc gcctcaatac gctgcccggc gctatcccac tgctggaaca
    17521 gttaccgatt ggttgtcgtc tggggccacg ttgcccgtat gcacaacgag aatgcattgt
    17581 gacgccacgt ttgacggggg cgaaaaatca tctctatgcc tgtcatttcc cgctgaacat
    17641 ggagaaagag tgagatgatc gaaacgctgc ttgaagtgcg taatttaagt aaaacgttcc
    17701 gctaccggac cggctggttt cgccgtcaga ccgtagaagc ggtaaaaccc ttgagcttta
    17761 cgctacgtga aggccagaca ctggcgatta ttggcgagaa tggttcgggt aaatccacgc
    17821 tggcaaaaat gctggcggga atgatagagc ctaccagcgg cgagttattg attgacgatc
    17881 atccactgca ttttggcgat tattccttcc gcagtcagcg tattcgcatg atttttcagg
    17941 atccttcgac ctcgttgaat ccccgtcaac gtatctcgca aattctggat tttccactgc
    18001 gcctgaacac cgacctggaa ccggaacagc gtcgcaaaca gattattgaa acgatgcgta
    18061 tggttgggct gctaccggat cacgtcagtt actatccgca tatgttggca cccggacaaa
    18121 aacagcgtct gggtctggcg cgcgcgctga tattgcgccc aaaagtcatt attgccgatg
    18181 aagcactcgc ttcactggat atgtcgatgc gttcgcagtt gattaatctg atgctggagt
    18241 tacaggaaaa acagggcatt tcgtatattt atgttaccca gcatatcgga atgatgaagc
    18301 acattagcga ccaggtgctg gtgatgcatc agggcgaggt tgtcgagcga ggcagcaccg
    18361 cggatgtgct ggcatcgccg ctgcatgaac tcaccaaacg gctgattgcc gggcactttg
    18421 gcgaagcatt gacagcggat gcgtggcgta aagatcgcta attttctgaa tcctccttcc
    18481 agattatcca cctcattctg acaagagatt aaatcttgtc agaatgaggt gatggataaa
    18541 ataaaatcaa atgcgcgtga tttaagacgc aatctcactt tgcaggaacg aaagctctgg
    18601 cgatatcttc gcagccgacg ttttggtgat ttcaaatttc gccgtcaaca tccggtgggg
    18661 agctacattc tcgattttgc ttgctgctcg gcgcgcgtag tcgttgagct ggatggtggg
    18721 cagcatgatt tggcagttgc ctatgatacc aggcgcacta gctggcttga gtcgcagggc
    18781 tggaccgtgc tgcgtttctg gaataacgag attgattgta atgaggaggc ggtgctggag
    18841 attattctgc aggaactgaa ccgccgatca ccctcaccct gaaagggcga gggggccgac
    18901 cgagccgaat agctgttgtg gtgaaaacat ggagacagtg ctggagaata ttcggcaagg
    18961 tctgaaccat cccagccatc gccataaaag ggttaggggc tgtatgagcc tgtttgttgc
    19021 tggggtaaca atatttgcac aatatggtcc cctcgcccct ctggggagag ggttagtgta
    19081 aggggaaaag cgcccaccct gccgcagcct gctccggtcg gacctagcaa ctatagctac
    19141 tcacagccag gttgattata ataaccattt atctgttcgt actgtttact aaaacgacga
    19201 atcgcctgat tttcaggcac aacaagcatc aacaataagg attaaagcta tgggttttct
    19261 ttccggtaag cgcattctgg taaccggtgt tgccagcaaa ctatccatcg cctacggtat
    19321 cgctcaggcg atgcaccgcg aaggagctga actggcattc acctaccaga acgacaaact
    19381 gaaaggccgc gtagaagaat ttgccgctca attgggttct gacatcgttc tgcagtgcga
    19441 tgttgcagaa gatgccagca tcgacaccat gttcgctgaa ctggggaaag tttggccgaa
    19501 atttgacggt ttcgtacact ctattggttt tgcacctggc gatcagctgg atggtgacta
    19561 tgttaacgcc gttacccgtg aaggcttcaa aattgcccac gacatcagct cctacagctt
    19621 cgttgcaatg gcaaaagctt gccgctccat gctgaatccg ggttctgccc tgctgaccct
    19681 ttcctacctt ggcgctgagc gcgctatccc gaactacaac gttatgggtc tggcaaaagc
    19741 gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg ggtccggaag gtgtgcgtgt
    19801 taacgccatc tctgctggtc cgatccgtac tctagcggct tccggtatca aagacttccg
    19861 caaaatgctg gctcattgcg aagccgttac cccgattcgc cgtaccgtta ctattgaaga
    19921 tgtgggtaac tccgcggcat tcctgtgctc cgatctctct gccggtatct ccggtgaagt
    19981 ggtccacgtt gacggcggtt tcagcatcgc tgcaatgaac gaactcgaat tgaaataatc
    20041 attctgttgg taaatattgg gcggcgttct gccgcccgtt atctctgtta tacctttctg
    20101 atatttgtta tcgccgatcc gtctttctcc ccttcccgcc ttgcgtcagg ataacgattt
    20161 cctttacgac caaggagcgc ccatggaaca acgccacatc accggcaaaa gccactggta
    20221 tcatgaaacg caatccagta ctgcggagta tgacgttctg cctctggtcc cggaagccgc
    20281 aaaggtcagc gatccctttc tgctcgacgt gatccttgat gaagaaacgc tagctccctt
    20341 cctttcatgg ctggtccctg cgcgcgttct tgcagtggaa ttgttccctg accagcttac
    20401 cgtgacccgt tcacagacct tcaccgctta tgaacgcttg tctacggccc tgacggttgc
    20461 tcaggtttgc ggcgtccagc ggttatgtaa ctactattcg gcgcgactta cgccgctccc
    20521 cgggcctgat tcctctaggg aaagcaatca taggttggca caaatcacgc aatatgcccg
    20581 ccaactggct agctcgcctt ctattatcga caaccgatcg cgccagcatc tgaatgacgt
    20641 cggtcttact gccagggact gtgtaatcat taaccaaatc attggtttta ttggctttca
    20701 ggcgcggacc attgcgacat ttcaggctta tctcgggcac ccggtacgct ggttacccgg
    20761 tctggaaata caaaactacg ccgacgcgtc actgtttgct gatgaatcaa tacgctggcg
    20821 aagcagctat gaggtggaaa aactacctga agagtacaca aaaagttcaa cagcagaact
    20881 ttgccaactg gccgaaacac tctctctcca ccctatttca ctttcccttc tcgaaaggtt
    20941 gttaaacagt acacgggtta atacacagcc ggataataag cttgcggcgt tgttatgcgc
    21001 gcggataaat ggcagtcctg cttgttttgc cgcctgtatg gattcatcaa atgaatataa
    21061 aaaaatcagc ccccttctgc gcaagggcga aaatgaaatt aaccaatggg ctgaccgtca
    21121 ttctgttgag cacgctaccg ttcaggcgat acaatggctg acccgagcac ccgatcgctt
    21181 tagcaccgcc cagttcagcc ctttactcga acacgaacaa tcatcaacgc agattattaa
    21241 tctgctggta tggagcgggc tgtgtggctg gataaatcgc ttaaaaatcg cgttgggtga
    21301 gacatattaa ccttgccgcg tcagacagat tcgcgtaaaa ctgtcagccg ctctaatggc
    21361 caccaaaata gacaattatg tttcaggaca acccgctgct agcgcagctt aaacagcaac
    21421 tgcattccca gacgccacgc gctgaagggg tggtaaaagc cacagaaaaa ggctttggct
    21481 tcctggaagt cgacgcgcaa aaaagttatt tcattccgcc gccgcagatg aaaaaagtca
    21541 tgcatggcga ccgaattatc gcggtgatcc acagtgaaaa agaacgtgaa tccgcagagc
    21601 cagaagaact ggttgaaccg ttcctgactc gtttcgtggg taaagttcag ggcaaaaatg
    21661 accgtctggc catcgttcct gatcatccac tcttaaaaga cgccattcct tgccgcgcag
    21721 cccgtggcct gaaccacgag tttaaagaag gcgactgggc ggttgccgaa atgcgccgtc
    21781 atccgctgaa aggcgatcgt tctttctatg ctgaattgac acaatacatc acttttggtg
    21841 acgaccactt tgtaccgtgg tgggttacac ttgcgcgcca taatctggaa aaagaagcac
    21901 cagacggcgt cgctaccgaa atgctcgatg aaggtctggt tcgtgaagat ctgaccgcgc
    21961 tggattttgt caccatcgac agtgccagca cagaagatat ggatgacgcc cttttcgcta
    22021 aggcgttgcc ggatgacaaa cttcagctga ttgtggcgat tgccgatcca accgcgtgga
    22081 ttgctgaagg cagcaagctg gacaaagccg cgaaaattcg cgcattcacc aactatctgc
    22141 ctggcttcaa catccctatg ctgcctcgcg agctttctga cgatctctgc tcactgcgcg
    22201 ccaatgaagt ccgcccggta ctggcatgcc gcatgacgct ctccgctgat ggcgccattg
    22261 aagataatat cgaattcttt gccgccacca tcgaatccaa agcaaagctg gtgtatgacc
    22321 aggtttctga ctggctggaa aataccggtg actggcagcc tgaaagtgaa gcaattgccg
    22381 aacaagtccg tttgctagcg caaatttgcc aacgccgcgg cgagtggcgt cataaccacg
    22441 cactggtgtt taaagatcgc ccggattacc gctttattct cggtgaaaaa ggtgaagtgc
    22501 tggatatcgt cgccgagcct cgtcgcattg ccaaccgtat cgtcgaagaa gcgatgattg
    22561 ccgctaacat ttgtgcggcc cgcgtactgc gcgataagct cggttttggc atctataacg
    22621 tgcatatggg ctttgatccg gcgaatgccg acgcgctggc agcgttgctg aaaacgcacg
    22681 gtctgcatgt cgatgccgaa gaagtgctca cgctggacgg tttctgcaaa ctgcgtcgtg
    22741 aactggacgc gcaaccaact ggtttcctcg acagccgcat tcgtcgcttc cagtcatttg
    22801 ctgaaattag cactgaaccc ggtcctcact ttggcctcgg tctggaagca tacgccacct
    22861 ggacttcgcc gatccgtaaa tatggcgaca tgatcaacca ccgtctgctg aaagcggtta
    22921 tcaaaggcga aactgcgacg cgcccacagg atgaaatcac tgtccaaatg gccgagcgtc
    22981 gccgtctcaa ccggatggca gaacgtgatg ttggtgactg gttatacgca cgcttcctga
    23041 aagacaaagc cgggaccgac acccgtttcg cagcggaaat tgtcgatatc agccgtggcg
    23101 gcatgcgtgt gcgtttggtt gataacggcg ctatcgcctt tattccggca cctttcttac
    23161 acgctatgcg cgatgaactg gtttgcagcc aggaaaacgg cactgtacaa attaaaggtg
    23221 aaacggttta caaagtaact gacgttattg acgtcaccat tgccgaagtc cgcatggaaa
    23281 cccgcagcat tattgcgcgc ccggtcgcgt aatctccttt cacggcctat tcctcatgga
    23341 tgggccgttt atttccccgc tctaccttcg ttatatccag gcagattttt taactgactt
    23401 tcgtttgaaa actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaacaat
    23461 gcgtttagcc gccgttaatt tcatgtaagt cat
//
LOCUS       DAJTOR010000050        23298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTOR010000050 DAJTOR010000000
VERSION     DAJTOR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.050"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            324..551
                     /gene="feoA"
                     /locus_tag="OO249_001764"
     CDS             324..551
                     /gene="feoA"
                     /locus_tag="OO249_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5636093.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            568..2889
                     /gene="feoB"
                     /locus_tag="OO249_001765"
     CDS             568..2889
                     /gene="feoB"
                     /locus_tag="OO249_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5636094.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2889..3125
                     /gene="feoC"
                     /locus_tag="OO249_001766"
     CDS             2889..3125
                     /gene="feoC"
                     /locus_tag="OO249_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5636095.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3328..4206
                     /gene="rpnA"
                     /locus_tag="OO249_001767"
     CDS             3328..4206
                     /gene="rpnA"
                     /locus_tag="OO249_001767"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5636096.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMGCLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(4235..5005)
                     /gene="bioH"
                     /locus_tag="OO249_001768"
     CDS             complement(4235..5005)
                     /gene="bioH"
                     /locus_tag="OO249_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5636097.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            5043..5726
                     /gene="gntX"
                     /locus_tag="OO249_001769"
     CDS             5043..5726
                     /gene="gntX"
                     /locus_tag="OO249_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5636098.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5785..6360
                     /gene="nfuA"
                     /locus_tag="OO249_001770"
     CDS             5785..6360
                     /gene="nfuA"
                     /locus_tag="OO249_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5636099.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6721..8037
                     /gene="gntT"
                     /locus_tag="OO249_001771"
     CDS             6721..8037
                     /gene="gntT"
                     /locus_tag="OO249_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5636100.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGLDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8082..10166)
                     /gene="malQ"
                     /locus_tag="OO249_001772"
     CDS             complement(8082..10166)
                     /gene="malQ"
                     /locus_tag="OO249_001772"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5636101.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(10176..12569)
                     /gene="malP"
                     /locus_tag="OO249_001773"
     CDS             complement(10176..12569)
                     /gene="malP"
                     /locus_tag="OO249_001773"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5636102.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQEVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLQDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEISEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13181..15886
                     /gene="malT"
                     /locus_tag="OO249_001774"
     CDS             13181..15886
                     /gene="malT"
                     /locus_tag="OO249_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR5636103.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIATVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(15929..16945)
                     /gene="rtcA"
                     /locus_tag="OO249_001775"
     CDS             complement(15929..16945)
                     /gene="rtcA"
                     /locus_tag="OO249_001775"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5636104.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(16949..18175)
                     /gene="rtcB"
                     /locus_tag="OO249_001776"
     CDS             complement(16949..18175)
                     /gene="rtcB"
                     /locus_tag="OO249_001776"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5636105.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            18364..19962
                     /gene="rtcR"
                     /locus_tag="OO249_001777"
     CDS             18364..19962
                     /gene="rtcR"
                     /locus_tag="OO249_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5636106.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWHPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(19944..20702)
                     /locus_tag="OO249_001778"
     CDS             complement(19944..20702)
                     /locus_tag="OO249_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5636107.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(20719..21549)
                     /gene="glpG"
                     /locus_tag="OO249_001779"
     CDS             complement(20719..21549)
                     /gene="glpG"
                     /locus_tag="OO249_001779"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5636108.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIIAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(21594..21920)
                     /gene="glpE"
                     /locus_tag="OO249_001780"
     CDS             complement(21594..21920)
                     /gene="glpE"
                     /locus_tag="OO249_001780"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5636109.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            22110..>23298
                     /gene="glpD"
                     /locus_tag="OO249_001781"
     CDS             22110..>23298
                     /gene="glpD"
                     /locus_tag="OO249_001781"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5636110.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRD"
BASE COUNT         5441 a         6368 c         5889 g         5600 t
ORIGIN      
        1 accaaactca taatatattc cggcaatatt tatcacttca ttaacaactg aaaccttaat
       61 taaacattag ccagtccggg taattcacta ttcgaattat attttcgctg cgatataacc
      121 ttgagccaca tcaacattga gtcagattat tattcaaacc aacattcgca cacattttaa
      181 gtattgctga tagaaaccat tctcattatc attgtgttat tgattattta atctctcctt
      241 tgttggcaaa tcatctggtc tcatgtcgct gtcaaacgcc ccatgaggta gttatccagt
      301 taatgagaaa caagtaggca cctatgcaat acactccaga tactgcgtgg aaaatcactg
      361 gcttttcccg tgaaatcagc ccggcatatc gccaaaaact gctttctctt ggcatgttac
      421 ctggctcctc ttttaatgtg gtgcgcgtcg ctccactcgg cgaccccatt catatcgaaa
      481 cccgtcgtgt gagcctggta ttacgcaaaa aagatctggc cttattagaa gtggaagcgg
      541 tttcctgtta atacggtgat aacaacaatg aaaaaattaa ccataggctt aattggtaat
      601 ccaaattctg gcaagacaac cttatttaac cagctcactg gcgcacgtca gcgtgtaggt
      661 aactgggctg gcgttaccgt cgaacgtaaa gaagggcaat tctccaccac cgatcatcag
      721 gtcacgctgg tggacctacc cggcacctat tctctgacca ccatctcatc gcagacctcg
      781 ctcgatgagc aaatcgcctg tcactacatt ttgagtggcg acgccgacct gctgattaac
      841 gtggtggatg cgtctaacct tgagcgtaac ctgtacctga cgctacaact gctggaactc
      901 ggcattccct gcattgtggc actgaacatg ctcgacattg ccgagaagca aaatattcgt
      961 attgaaattg atgctctgtc ggcgcgtctg ggctgtccgg tgatcccgct ggtttcaacc
     1021 cgtggtcgcg gtattgaagc gctcaagctg gcgattgatc gctataaagc taacgagaat
     1081 gtggaactgg tgcattacgc acagccgctg ctcaacgaag cagattcact ggcaaaagtg
     1141 atgccttccg acatcccgct gaaacaacgt cgctggctgg gcctgcaaat gctggaaggc
     1201 gatatctaca gccgcgccta cgccggtgaa gcgtcgcagc atctggatgc cgccctcgcc
     1261 cgtctgcgta atgagatgga cgatccggcg ctgcacattg ccgatgcgcg ttaccagtgc
     1321 attgctgcca tctgtgatgt ggtaagcaac accctgacgg cagaacccag ccgtttcacc
     1381 actgcggtag ataaaatcgt gctcaaccgt ttcctcggtc tgccgatttt cctctttgtg
     1441 atgtacctga tgttcctgct ggctatcaac atcggcgggg cgttacagcc gctgtttgac
     1501 gtcggctccg tggcgctatt tgtgcatggt attcaatgga ttggctacac gctccacttc
     1561 ccggactggc tgactatctt cctcgcccag ggcctgggtg gcggcattaa caccgtgctg
     1621 ccactggtgc cgcagattgg catgatgtac ctgttcctct ccttccttga ggactccggg
     1681 tatatggcgc gtgcggcgtt tgtgatggac cgtctgatgc aggcgctggg cttgccgggg
     1741 aaatcctttg tgccgctgat cgtcggtttc ggttgtaacg taccgtcggt aatgggtgca
     1801 cgtacgcttg atgcaccgcg tgaacgtctg atgaccatca tgatggcacc gtttatgtcc
     1861 tgcggcgcgc gtctggctat cttcgcagta ttcgcggctg ccttcttcgg gcagaacggt
     1921 gcgctggcgg tcttctcgct gtatatgctg ggtattgtga tggcggtgct gactggcctg
     1981 atgctcaagt acaccatcat gcgcggtgaa gcgacgccgt ttgtcatgga gttgccggtc
     2041 tatcatgtac cacacgttaa aagcctgatt atccagacct ggcagcgtct gaaaggcttc
     2101 gttctgcgtg ctggtaaagt gatcatcatc gtcagcattt tcctgagcgc tttcaacagc
     2161 ttctcgctga gcgggaaaat cgtcgataac atcaacgact cggcgctggc gtccgtcagc
     2221 cgggtgatca ccccggtctt caagccaatt ggcgtgcatg aagataactg gcaggcaacg
     2281 gttggcctgt ttacaggtgc catggcgaaa gaagtggtag tgggtacgct caacaccctc
     2341 tacaccgcag aaaatattca ggacgaagag ttcaatccgg cagagtttaa cctcggtgaa
     2401 gagctgttca gtgcgataga tgaaacctgg cagagcctga aagacacctt cagccttagc
     2461 gtactgatga accccattga agccagcaaa ggcaacggcg aaatgggtac cggggcgatg
     2521 ggcgtgatgg atcagaaatt cggtagcgca gcagcagctt acagctacct gattttcgtc
     2581 ctgctgtatg taccatgtat ctcggtgatg ggagccatcg cccgtgaatc aagccgtggc
     2641 tggatgggct tctccatcct gtgggggctg aatatcgctt actcgctggc aacattgttc
     2701 tatcaggtcg ccagctacag tcagcaccca acttacagcc tggtgtgcat tctggcggtt
     2761 atcctgttta acatcgtggt tatcggtctg ctgcgccgcg cgcgtagccg ggtggatatc
     2821 gaactgctgg caacccgcaa gtcggtaagc agttgctgcg cagccagcac caccggtgat
     2881 tgccattaat ggcttcactt attcaggtgc gcgatttgct ggcgttacgg ggccgtatgg
     2941 aagcggccca gataagccag acattgaaca ctccacagcc aatgattaac gccatgctgc
     3001 aacaactgga aagtatgggc aaagccgtgc ggattcagga agaacctgac ggctgcctct
     3061 ctggcagttg taaaagctgc ccggaaggaa aagcctgtct gcgcgagtgg tgggcgcttc
     3121 gttaacctta ctccatcgcc tgttttggaa agcccggtat gcgtctgcat ccgggctttt
     3181 ttgcgtgcgg ctttccataa aaatgcaact cttgcagcac ggcgtaagtt cctttgaaag
     3241 catctcgcag ggatgaaaac tcgctaatac acaggtgtgg agtggcgcgt agagtcgcgg
     3301 cattcaaaca acaggtgaag gaacgccatg agcaaaaagc agagttccac cccacacgat
     3361 gcgctgttca aactcttttt acgccaaccg gacacggctc gtgattttct tgcgtttcat
     3421 ttaccggcac ccattcacgc gctttgtgat atgaaaaccc tcaagctgga gtcgagcagc
     3481 tttattgatg acgatctgcg tgaaagctat tccgatgtgc tgtggtcggt gaaaacggaa
     3541 caaggaccag gatacatcta ttgtctgatt gaacatcaaa gcacctcaaa caaactgatc
     3601 gcatttcgca tgatgcgtta cgctattgcc gcaatgcaaa atcaccttga tgctggatac
     3661 aaaacgttgc cgatggtggt gccattgttg ttttaccacg gtattgaaag cccctatccc
     3721 tattcgctgt gttggctgga ttgtttcgcc gatcccaaac tggcaaggca gctttatgcc
     3781 tccgcatttc cgctgattga tgtcaccgtc atgcctgatg atgaaatcat gcagcaccga
     3841 cgtatggcgc tgctggagtt aattcaaaaa catattcgtc aacgcgacct gatggggcta
     3901 gtagagcaaa tgggctgctt attaagtagt ggatacgcta atgacagaca aatcaaaggg
     3961 ctgtttaatt acatactgca aaccggcgac gcggtacgtt ttaacgattt tatcgacggc
     4021 gttgccgaac gttcaccgaa acacaaggag agtttaatga caattgcgga aagattgcgg
     4081 caggaggggg aacaatccaa agccctgcat atagccaaaa taatgcttga atccggagtc
     4141 cctcttacag acatcatgcg ctttaccggg gtgtcagaag aagagttggc tgcggcgagc
     4201 cagtaaattt ctgtctcgcc atttcaaaag ttgcctacac cctctgcttc aacgccacca
     4261 gcaggtgaca aaactcgacc ggatgcgaaa taaacggcgc atgggccgct ttggcgaaga
     4321 tatatgattt gctgcgaggc cagagtttat ccagcatcgg caccactttg cgcggcacca
     4381 gaccatcgag atagccatac aatcgcaaaa acggcatggg cacgttttgc agcggctgac
     4441 ggagatcaac cgttttcagg atttccagcc cgccattaag cacgtcaacc tccggcatcg
     4501 gtaacgccag aacggttttc ttcaacgccc gcgcatcctg gcgcgccgtt tcagtcccca
     4561 tggtttgtaa cgccaggaac cgctccactg tacgctgaaa atcatcactg agttgctgct
     4621 gaaatcccgc cagcacgtcc ggttttatcc ccggccaatc gtcacgagca ctaaaacaag
     4681 gtgacgacgc cacggtgacc agcgcctgaa cacgctcggg atgggttaac gcaatctggc
     4741 ttgccaccag cccgcccaga ctccagccta accaaatggc tttatcaggt gcctgttgca
     4801 gcacggcttc ggccatatca gcaagtgaca gcgcaccaaa tccctggctg cgcccgaagc
     4861 cgggcagatc aacaaggtgc agcgtaaaat gcgagctaag ttcctcgtca atgcaacgcc
     4921 acacttcggc attcagtccc catccgtgca gcagcacaag atgaacattc ccctgacctt
     4981 tggtctgcca ccagatgtta ttcatccgct attgttctct tttgacttac aaggatgaac
     5041 atatgctaac agtaccggga ttatgctggc tatgccgaat gccgctggcg ttaggtcatt
     5101 gggggatttg ttcggtctgc tcacgcgccg cccgtacaga taaaacgtta tgcccacaat
     5161 gtggattacc cgccacacac tcacatcttc cctgcggtcg ctgcctgcaa aaaccaccgc
     5221 cctggcaaag actggtcacg gttgccgact atgcgccgcc gttaagtccg cttatccacc
     5281 agcttaaatt ttcccggcgc agtgaaatcg ccagcgccct gtcacgtctg ttactattgg
     5341 aagtcttaca cgctcgtcgc accaccgggt tgcaattgcc ggatcgcatc atcagcgttc
     5401 cgttatggca gcggcgtcac tggcgtcggg gatttaatca gagcgatttg ctgtgtcagc
     5461 cgttatcacg ctggttgcac tgccgatggg atagcgaagc cgtcacacga acacgggcca
     5521 ctgcgaccca gcattttctc agtgcccggc tacgcaagcg caacctgaaa aatgcctttc
     5581 gtcttgaatt gcccgtgcaa ggtcgccata tggtgattgt ggatgatgtc gttaccaccg
     5641 gaagtaccgt cgcagagatt gcgcagttgc ttttacgcaa tggtgcggcg actgtccagg
     5701 tctggtgcct ttgtcgaacc ttgtagagcc tcgatgatag gcgtattata accaactaaa
     5761 atagtcaact attaggccat tactatgatc cgtatttccg atgctgcaca agcgcacttt
     5821 gccaaactgc tggcaaacca ggaagaaggg acacaaatcc gcgtatttgt gattaaccct
     5881 ggcacgccta acgctgaatg tggtgtttct tattgtccgc cggatgccgt ggaagccacc
     5941 gacactgccc tgaaatttga cctgctgacc gcgtatgttg atgagttaag cgctccttac
     6001 ctggaagatg cagagatcga ttttgttacc gaccagttgg gttctcagct aacactgaaa
     6061 gcgcctaacg ccaaaatgcg taaagtggca gacgatgcac cactgatgga gcgcgtggag
     6121 tatatgctgc agtcgcagat caacccacag cttgctggtc acggtggccg cgtttcactg
     6181 atggaaatca ccgaagacgg ttacgccatt ctgcaatttg gcggcggctg taacggttgt
     6241 tccatggtcg atgtgacgct gaaagaaggg atcgagaagc agctgctaaa cgaattcccg
     6301 gagctgaaag gtgtgcgcga tctcaccgaa caccagcgcg gcgaacactc ctactactaa
     6361 gttatcccct cttttgtaga ttgcccgatg cgacgctaaa gcgtcatttc gggcctacgt
     6421 tgaacatgtg ccagacgtgg gcgcagcatt gctttggcaa atccctccct tcgtttctcc
     6481 gcgataatat gaccaacctc tcataattta aatttactcc gctctggtga ttctcaaacg
     6541 ccagatgtta ccggtatcat tcacatgggt accaaacata ctcctgacat ctgactacaa
     6601 taattagttt tagtgggtat cagtcgtggt gccgcaatat ctctgttccc gattgggata
     6661 attagagttt gtcgtcagaa aattgacgtt acccgcaaca aatgaaaggc caggtaaatc
     6721 atgccattag tcattgttgc tatcggtgta atcttgttgt tgctcctgat gatccgtttc
     6781 aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg ctgttggatt aatgcaaggg
     6841 atgccgctgg ataaagttat tggctccatc aaagccggtg tcggcgggac gctcggtagc
     6901 cttgccctga tcatgggttt tggcgcgatg ctgggcaaaa tgctggcaga ctgcggtggc
     6961 gcacaacgta tcgccaccac cctgattgcc aaatttggta aaaaacacat ccagtgggcg
     7021 gtggtactga ccggttttac cgttggtttt gccctgttct atgaagtggg ctttgtgctg
     7081 atgctgccgc tggtgtttac catcgcggct tctgcgaata ttccactgct gtatgttggt
     7141 gtaccaatgg cagctgcact gtctgtgacc cacggcttcc tgccaccgca tccgggtccg
     7201 actgcgattg ccaccatttt caatgccgac atgggtaaaa ccctgctgta cggtactatt
     7261 ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg ctcgcgtgtt gaaaggtatc
     7321 gataagccga tccctgaagg tctgtacagc gcgaaaacct tcagcgaaga agagatgccg
     7381 agctttggcg tcagcgtctg gacctctctg gtgccggtag tgctgatggc gatgcgtgcg
     7441 attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc cggtagcgga gttcctcggt
     7501 gacccggtaa tggctacgct gattgccgtg ctgattgcga tgttcacctt tggtctgaac
     7561 cgtggtcgtt caatggatca gattaacgac acgctggttt cttccatcaa aatcattgcg
     7621 atgatgctgt tgatcatcgg tggtggcggt gcgttcaagc aggtgctggt agacagcggc
     7681 ttggacaaat acattgcttc catgatgcac gaaaccaaca tttctccgct gctgatggcc
     7741 tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg caaccgttgc ggcaatcact
     7801 gcgggtggta tcgcggcacc gctgattgca acgacgggtg ttagcccgga gctgatggtt
     7861 attgcggtgg gttccggtag tgtgattttc tctcatgtga acgatccggg cttctggctg
     7921 ttcaaagagt actttaacct gactatcggc gagaccatca aatcctggtc gatgctggaa
     7981 accattatct cggtgtgcgg tctggtaggc tgtctgctgc tgaatatggt gatttgataa
     8041 cactgactgc cggatgcatc gcatccggca tcaaccgcac tctactgctt cttcgctgca
     8101 gctctgcgcc gtctgtccaa atccttcagc aacttattca cgccatcatc ggcaaacatc
     8161 gtctcaagcg ttgcggaaag cttgcgtcgc cagtttttat actggtaact ggtgccagga
     8221 atattcaccg gttcggccat atccagccag tcttccggct gtagtcctaa cagagcactg
     8281 ttactgtcgg caatgtagcg ctgcaaacca cgattaagcg tcggcgtcat cgacatcaac
     8341 gatgccttat gcccggcacg ttttggcaga caaccatatt tatgcagtgc atccagcagc
     8401 ccttgcttcg ccagttcgcg atcctgatac agaccgcgca gtaccacttc atccggatac
     8461 agccccaggg ttttgcccaa cgttagatcc ccgctctccc agtaaccgcg cagcgtaggc
     8521 aggtcatgtg tcgccgcaac cgccatcgac tgctccggat acgctttcgg tgcacggaac
     8581 gtcttctcgt ggtcgttttc gaaatagagc actttgtagg agtacacacc gctgctgcgc
     8641 agcttaccga caatctctac cggtacggta ccgagatctt caccaatcac catacagcga
     8701 tgacgtttac tttcgagtgc cagaatcgag agcagatcat ccaccggata gtgaacatac
     8761 gcgccctgat ctgccgtctc gccatacggt atccaccaca aacgcagcat cgacatcaca
     8821 tggtcaattc gtaatgcgcc gcagttttgc atattggcac gcaacagctc gataaacggt
     8881 tcataggcac gcgcggtgat gatatgcggg tccattggcg gtaatcccca gttctgcccc
     8941 aacgggccga ggatatccgg cggcgcgcca accgacgctt tcaggcaata tagttcacgg
     9001 tcacaccagg tttccgcccc accttccgct acgccaaccg ccagatcacg atacaagcca
     9061 atcggcattt catagccctg gcttatctcc cagcaggcgg caaactggct gtaagccagc
     9121 cactgcaacc agagataaaa atcgacgtca tcacgatgtt cttcgcagaa ctgacgcact
     9181 tctggtgaat ccacgttctg atacatctct ggccatgcag gccagcccca gcgcatttcg
     9241 tcctctttca cttgctgggc atgtagcgca tcaaaggctg cctgccagaa caggctgtcg
     9301 ccctgctctg caacaaactg gcgaaacgcg gtcatctgct catcatcacg ttgcgcgaaa
     9361 cctttccacg ccattcgtaa tgctgtcatt tttagggcgg taaccgtgga gtaatcgacc
     9421 cagtcggcat cgcgcgcctg ttgcagcgtc tgttgcgtgg tcggcaactg ccaccaggcc
     9481 tgagcctctt cgctaagatg gaaatcttca acggcgttaa cgtcgatata aatcacattc
     9541 agccaacggc gagaagacgg actgtatggg ctggcactct ccggatttgc cggatagagc
     9601 gcatgaatcg ggttcaggcc gatgaacgac ccgccacgtt ttgccacatc caccagcatc
     9661 gctttgagat cgccaaaatc cccaataccc cagttttttt ccgatcgcag cgtataaagc
     9721 tgaacgcagg caccccacag cttttgttta ttcagcaacg cctgcggttc gtaacagcgt
     9781 ttcggggcga caatcacccg gcaatgcgcg cgctggtcgt cctgggtgag tgtcagcgtg
     9841 tgataacctt ccggcagctt cgtcggtaga ttgaacgctt tgccccccgt tacatggcct
     9901 ttgtactgtg ttccttcttc ggtggtcagc agccagctat attcgccgct gccctccacc
     9961 accatcggca tttttttgcc gctggtataa accatgacat tcgggactgg cgttaccgcc
    10021 actttcgtgg cggtacgttg atgcatcgcg tcaagcaaac gccgtttggt ttcggcgcta
    10081 atcgactgcg gtttaccgtg ggcattgatg taattggggc taatccccgc cgccagcgcg
    10141 gcattatcca gacgtttgct ttccatcgag cttccttagc gttttgcctg ccagatacga
    10201 gcctgataat cgcgaataga gcgatccgag ctaaacatac cgcagcgggc ggtattgagg
    10261 atcgccgcgc gagtccaggc ctcctggtcg cggtacagca catccacctg cttttgtgcc
    10321 tcaacatagg ctgcgaaatc cgccatcacc agatacggat cgccgccttg tttgccgata
    10381 ctgtgcagca tctggtcgaa ggcatgctta tcgccgtcgc tgtatttacc gctttccagc
    10441 tctttcaata ctgcgtccag caccttatct ttcttccgcc atttcaccgg gtcgtagcct
    10501 ttggccagaa ttgccttcac ttgttccacg gtatgaccaa aaataaagat attttcttca
    10561 ccgactttct cggagatttc aacgttcgca ccatccagcg taccgacagt aagcgcacca
    10621 ttgagcgcca gtttcatatt gccggtaccg gaagcttctt tacctgcagt cgaaatttgt
    10681 tcggagatat ccgccgccgg gatcagtttt tccgccgccg aaacgcaata atcctgcagg
    10741 aacaccacct tcaacttatc gccaaccagt ggatcgttgt tgatcacgtc agccactttg
    10801 ttgatcgcaa agataatatt cttcgccagg tagtagcccg gtgccgcttt cgcgccgaag
    10861 aggaagacgc gcggtacgcg atcagcctgc gggttttcac gaatttcttt gtacaacgcc
    10921 agaatatgca gcagattcag gtgctggcgt ttgtactcat gcagacgttt aatctgaata
    10981 tcgaaaatcg cctgtggatt gatctcaata ccggtacgaa ctttcacaaa ctccgccaga
    11041 cggactttat tcgcctgctt gatctcgcga tattgctgac ggaatttcgc atcatcagcg
    11101 aatttttcca gattgatcag ctgatcgaga tcgttagccc actctttttg cagtaattta
    11161 tccaacagag ccgccagtgc agggttgcac tgtttgatcc agcgacgtgg ggtaataccg
    11221 ttggtgacgt tatggaattt gttcggccat agctggtgat attccgggaa cagatctttc
    11281 accaccagat ccgagtgcag cgccgcaaca ccgttcaccg cgaaaccgcc aaccacacac
    11341 aggttcgcca tatgcacttg tttatcgtgc accaccgcca gtttggccca cactttttca
    11401 tcgcccggcc aggttttctc caccagcgtt ttaaagcgag tattaatttc gttaataatc
    11461 tgcatatggc gcggcagtaa gcctttcacc agtttcacat cccagcgttc cagcgcttct
    11521 ggcatcaggg tatggttggt gtaagcgaaa gttttgctgg taatggccca ggcgtcatcc
    11581 cagctcatct ggtgctcatc gatcagcacg cgcagcagtt ctggaatcgc gatagttggg
    11641 tgggtatcgt tcagctgaat aacttcataa tccgccagtt cgtgcagttt acgccccgcc
    11701 agatgatggc gacgcaaaat atccgctacc gaacaggcac actggaagta ctgctgcatc
    11761 aagcgcagct ttttaccggc agtatggttg tcgtttggat agagaacttt ggtcagtttt
    11821 tccgcattga tgccctgctg ttcggcacgc aggaaatcac cgtcgttaaa tttagtcaga
    11881 tcaaacggat gcgcgtgcgt cgcctgccac agacgcagcg gctgcgccac gccattacga
    11941 tagccgacaa cggggagatc ccacgcttga ccggtaatgg taaactccgg ctcccagcgt
    12001 ccgtctttcg tcactttacc gccaatccct acctgcacat ccagtgcttc gttgtggcgg
    12061 aaccacgggt agttactacg atgccagtca tccggcgctt caacctgttt gccatcgaca
    12121 aaagactggc ggaacaaacc atattgatag ttcagaccgt aacccgtcgc agactgaccg
    12181 atagttgcca ttgagtcgag gaagcacgcc gccagacgtc ccagaccacc gttacccagc
    12241 gccgggtcga tctcttcttc cagcaggtcc gtcagattga tgtcataagc cttcaacgaa
    12301 tcctgtactt cctgatacca gccgagattc aacaggttgt tgcccgtcag gcgaccaatc
    12361 aaaaactcca ttgagatgta gttaacatgt cgctgattcg ccaccggctt ggcgaatggc
    12421 tgagcacgca gcatttcggc cagtgcttca ctcactgcta gccaccactg gcgaggagtc
    12481 atttcagccg cagaatttaa gccataacgc tgccactgac gtgaaagcgc ttcctgaaat
    12541 tgcttatcgt taaaaatagg ttgtgacata ggagttccac ttttcttaga ttttcaacac
    12601 aacgttatcg ctagtttgcc aggctcgatg ttgaccttcc tcatcctgcg ggggattagg
    12661 cagggaggag ttgcggggat gagcaaggaa atgtgatctc aaccacttaa agctagtgca
    12721 aaccacagga ttagcagcaa atcaatgcaa tacagcgcag aaaatctgta tctaagtgca
    12781 aaaaatggcc gttgcgtatt ttcaaaaagc ggaaggtaac tctataaatt aagtaaagga
    12841 gtgaaacagt ttcacaagta aaatatccag tgtgctccat ctcattctta atagatttat
    12901 taagatcatc tttttagatg gcactttcat caggaatgaa gaagaaaccc ttgcttaaat
    12961 gaatctgatg aacataaggg aaaccagtat tcacgctgga tcagcgtcgt tttaggtgag
    13021 ttgttaataa agatttggaa ttgtgacaca gtgcaaattc agacacataa aaaaacgtca
    13081 tcgcttgcat tagaaaggtt tctggccgac cttataacca ttaattacga agcgcaaaaa
    13141 aaataatatt tcctcatttt ccacagtgaa gtgattaact atgctgattc cgtcaaaact
    13201 aagtcgtccg gttcgactcg accataccgt ggttcgtgag cgcctgctgg ctaaactttc
    13261 cggcgcgaac aacttccggc tggcgctgat cacgagtcct gcgggctacg gaaagaccac
    13321 cctcatttcc cagtgggcgg caggcaaaaa cgatatcggc tggtactcgc tggatgaagg
    13381 tgataaccag caagagcgtt tcgccagcta tctcattgcc accgtgcagc aggcaaccaa
    13441 cggtcactgt gcgatatgtg agacgatggc gcaaaaacgg caatatgcca gcctgacgtc
    13501 actcttcgcc cagcttttca ttgagctggc ggaatggcat agcccacttt atctggtcat
    13561 cgatgactat catctgatca ctaatccagt gatccacgag tcaatgcgct tctttattcg
    13621 ccatcaacca gaaaatctca ccctggtggt gttgtcacgc aaccttccgc aactgggcat
    13681 tgccaatctg cgtgttcgtg accaactgct ggaaattggc agtcagcaac tggcatttac
    13741 ccatcaggaa gcgaagcagt tttttgattg ccgtctgtca tcgccgattg aagccgcaga
    13801 aagcagtcgg atttgcgatg acgtttccgg ttgggcgacg gcactacagc taatcgccct
    13861 ctccgcccgg cagaataccc actcagccca taagtcggca cgccgcctgg cgggaatcaa
    13921 tgccagccat ctttcggatt atctggtcga tgaggttttg gataacgtcg atctcgcaac
    13981 gcgccatttt ctgttgaaaa gcgccatttt gcgctcaatg aacgatgcgc tcatcacccg
    14041 tgtgaccggc gaagaaaacg ggcaaatgcg tctcgaagag attgagcgtc aggggctgtt
    14101 tttacagcgg atggatgata ccggcgagtg gttctgctat cacccgctgt ttggcaactt
    14161 cctgcgccag cgctgccagt gggaactggc ggcggagctg ccggaaatcc accgtgccgc
    14221 cgcagaaagc tggatggccc agggatttcc cagcgaagcg attcatcatg cgctggcggc
    14281 aggcgatgcg ctgatgctgc gcgatattct gcttaatcac gcctggagtt tgttcaacca
    14341 tagcgaactg tcgctgctgg aagagtcgct taaggccctg ccgtgggaca gtttgctgga
    14401 aaatccgcag ttagtgttat tgcaggcgtg gctaatgcaa agccaacatc gctatggcga
    14461 agttaacacc ctgctagccc gtgctgaaca tgaaatcaag gacatcagag aaggcaccat
    14521 gcacgcagaa tttaacgctc tgcgcgccca ggtggcgatt aacgatggta atccggatga
    14581 agcggaacgg ctggcaaaac tggcactgga agagctgcca ccgggctggt tctatagccg
    14641 cattgtggca acctcggtgc tgggtgaagt gctgcactgc aaaggcgaat tgacccgctc
    14701 actggcgcta atgcagcaaa ccgaacagat ggcacgccag cacgacgtct ggcactacgc
    14761 cttatggagt ttaatccagc aaagtgaaat tctgtttgcc caagggttcc tgcaaaccgc
    14821 gtgggaaaca caggaaaaag cgttccagct gatcaacgag cagcatctgg aacagctgcc
    14881 gatgcatgag tttctggtgc gcatccgagc acaactgtta tgggcctggg cacggctgga
    14941 tgaagccgaa gcgtcggcgc gtagcgggat tgaagtcttg tcgtcttatc agccacagca
    15001 acagcttcag tgcctggcaa tgttgattca atgctcgctg gcccgtggtg atttagataa
    15061 cgcccgtagc cagctgaacc gtctggaaaa cctgctgggg aatggcaaat atcacagcga
    15121 ctggatctct aacgccaaca aagtccgggt gatttactgg caaatgaccg gcgataaagc
    15181 cgccgctgcc aactggttgc gtcatacggc taaaccggag tttgcgaaca accacttcct
    15241 gcaaggtcaa tggcgcaaca ttgcccgcgc acaaatcttg ctgggcgagt ttgaacctgc
    15301 ggaaattgtt ctcgaagaac tcaatgaaaa tgcccgcagt ctgcggttga tgagcgatct
    15361 caaccgtaac ctgttgctgc ttaatcaact gtactggcag gccggacgta aaagtgacgc
    15421 ccagcgcgtg ttgctggacg cattaaaact ggcgaatcgc accggattta tcagtcattt
    15481 tgtcatcgaa ggcgaagcga tggcgcaaca actgcgccag ctgattcaac tcaatacgct
    15541 gccggaactg gaacagcatc gcgcgcagcg tattctgcga gaaatcaatc aacatcatcg
    15601 gcataaattc gcccatttcg atgagaattt cgttgaacgt ctgctaaatc atccggaagt
    15661 gccagaactg atccgcacca gcccgttgac ccaacgtgaa tggcaggtcc tggggctgat
    15721 ctactccggt tacagcaatg agcaaattgc gggcgagctg gaagtggcgg caaccaccat
    15781 caagacgcat atccgcaatc tgtaccaaaa actcggcgtc gcccaccgtc aggccgcggt
    15841 acaacacgcg cagaaactgc tgaagatgat gggatatggg gtgtgagttt agccggataa
    15901 tgcattggat ccggtttaca tctttacatc attcaatact cacccgcgtt acgccatctg
    15961 tttctatcaa accaaaccgc accggcaaga aacgctccac cacggcgata ttggtcagca
    16021 gatggcatga aggatgggcg accgtaaatt ctcccgcgcc cgccagcgcc atcggtagca
    16081 ccagctggtc ggcgagatat tcccccactg ccgccgggct tgccaggtag cgtttcacct
    16141 ctttcactaa ctgcgcggct accacctcgg cactgacgcg cttttcaccg acgacgaaaa
    16201 agcgttcagt gatattttca ctttcgactt ccagcgagac ggtattaccc ggcccctgat
    16261 cgcgcggcag gttatgaata ttctgttcat gcagggaaaa actccccgcc agtgtagcga
    16321 tttcacgctc agcaacatgg cgcggcacac cagctaatag aacttctcca cgcatctgca
    16381 caatgttccc gcgctcgcca agttgcaagg tgttaaacga tgccacgggc gagacttccg
    16441 ttgccaccac gccgcctccg gcaggataaa aaccgtgacg taacagcgtg gtttgctgat
    16501 gaattcctat tttcgccagc agcggctcca gcacccggcg gataaaatcc gcaggcggcg
    16561 ccgacgggtt atcggtgcct ccgctcactt caacacgcga aggtccatcg gcaaaccaca
    16621 gcgcgggcag caccgtttgc agcaccagcg tacagcttcc ggcactaccg atagcaaagc
    16681 ggtactcgcc gccgcgcacg gcgccaggcc ggaagaccag acgctgcgac cccagctccg
    16741 cgccttccac cgttgcccta caaatttccg cagccgcttt taccgcggtc agatgctggc
    16801 gcaacagccc cggtttcgcc cgcccggcac gaatgctggt gatggtaaat ggctggccgg
    16861 ttatcatcga caggctcagc gccgagcgca gaatctgccc gccaccttcg ccctgtgcgc
    16921 catccagcgc aatcatcctt ttcatcattt atccttttac gcacaccacc tgacgcaggg
    16981 tatagataac ttccaccaga tcgctttgtg ccgtcatcac cgcatcaata tctttatacg
    17041 ccatcgggat ttcgtcgatc acttcggcat ctttacggca ttccacatgc gcggtggcac
    17101 gaatttgatc ttccacgctg aacagttttt tcgctttagt tcggctcatt acccgcccgg
    17161 caccgtggct gcacgaacag aacgactctt catttcccag cccacggacg ataaagcttt
    17221 ttgctcccat cgaaccggga ataattccat attgaccagc acgcgcagac accgcgcctt
    17281 tacgcgtcac gtagatctct tcaccaaagt gctgttcttt ttgcacatag ttgtggtgac
    17341 agttgatctc ttccatcgcc agcgtttgtg gctgtctgac cgttttctgc gtaatgctct
    17401 gcaatgccgt taccacgttt tccatcatca catcgcggtt aaggctggca aaaagctgcg
    17461 cccaggccac ggctttcagg taatcatcaa agtattccgt accttccata aagtacgcca
    17521 gatcacgcga cggcaacgtc tcaagcgttt cctgcatctc tttttgtgcc agatcgataa
    17581 agtaagtccc gatggcgtta ccaattccgc gtgaaccgga gtgcagcata atccacacct
    17641 ggtccgactc atcaaggcag attttaataa agtggttacc ggttcccagc gttcccaggt
    17701 gtttatagtt attggtattc aggaaacggg gatatttttg cgttaaccac tgataaccgg
    17761 cttcaagctc agcccatttc gcatcgacgt tgacaggcgg gttttcccat gcgcctttat
    17821 cacgtttaca acgcccggta gtacgcccgt gcggcacggc cgtttcaatc gcctgacgca
    17881 gctctgccag gttttcaggc aggtcttccg ccgttaacgc ggtacgcagc gcgttcattc
    17941 cacagccaat atccacgccc accgccgccg gaataatcgc ccctttggtc gggatcacgc
    18001 taccaatggt ggaatcttta cccaggtgta catcaggcat taccgcaata tgtttgaaaa
    18061 taaacggcat cttcgccgta ttaataagtt gctgacgcgc atcggcttct accggcacgc
    18121 ctttggtcca catttttacc ggggcatttt cagtggtcag taattcgtaa ttcattttgt
    18181 ttttctcttt tcgttgtttg ctgtcctgat aattgcaacc gtcgtgccag aaaattaaat
    18241 aagtaaatta attttttaat tcattgtttt taaaaagatt atatctttat gtccgtaacc
    18301 ggagatttcc cgcaaagccg atttaccgat aatgaaatat cgtcttttat aaggatatct
    18361 aagatgcgta aaacagtagc ttttggcttt gtcggaacgg tgctggatta tgccgggcgc
    18421 ggcagtcagc gctggtcaaa atggcatccg acactctgtt tatgccagca agaatcgttg
    18481 gtcatcgatc gactggaatt attgcatgac gcccgctcgt gctcgctatt tgaaacgctt
    18541 aaacgcgata tcgccagcgt ttcgccagaa acagaagtgg tgagcattga gattgaactg
    18601 cataacccgt gggatttcga agaggtttac gcctgcctgc atgatttcgc ccgtggttac
    18661 aagtttcagc cagaaaaaga agactattta attcacatca ccaccggcac ccacgtcgcg
    18721 cagatttgct ggtttctgct ggcggaagcg cgttatctac ccgcccggtt gatacaaact
    18781 tcaccaccgc gcaaaaaaga acaaccgcgc ggtgccggtg aagtgacgat tatcgatctc
    18841 gatctaagcc gttataacgc catcgccagc cgctttgccg aggaacgcca gcaaacgctt
    18901 gattttctta agtccggcat tgccacgcgt aacccccact tcaaccgcat gattgagcag
    18961 attgaaaaag tggcgattaa atcccgcgca ccgatcctgc ttaacggccc aaccggtgcg
    19021 ggaaagtcat ttctggcgcg acgtatattt gaattaaaac aggcgcggca tcagtttagc
    19081 ggcgcgtttg tggaagtgaa ctgcgccacc ctgcgcggcg ataccgccat gtcgacgctg
    19141 tttggtcatg tgaaaggcgc gtttaccggg gcgcgggaat ctcgtgaagg tttattacgc
    19201 agcgccaacg gcggaatgtt gttttttgat gagattggcg aactgggcgc agacgaacag
    19261 gcaatgctgc taaaagccat tgaagagaaa accttttacc cgtttggcag cgatcgccag
    19321 gtgagcagtg attttcagct tatcgccgga acggtgcgcg atttgcgcca gctggttgcc
    19381 gaaggcaaat ttcgcgaaga tctgtacgcg cggatcaatc tctggacctt caccctgccg
    19441 ggtctacgcc agcgccagga agatattgaa ccgaaccttg attatgaagt ggagcgccac
    19501 gccacactca ctggcgacag cgtgcgtttt aacaccgaag cgcggcgcgc ctggttggct
    19561 tttgcgacct ctccccaggc aacatggcgc ggtaactttc gcgaactttc tgccagcgtc
    19621 acgcggatgg ccacctttgc cactagcgga cgcatcactc tggacgtggt tgaagatgag
    19681 ataaaccgtc tgcgctataa ctggcaggag agccgcccct ccgcgcttac ggcgttgctg
    19741 ggcgcagagg cagaaaacat cgatctcttc gaccgtatgc aactggaaca cgttatcgct
    19801 atctgccgcc aggcaaagtc gctttctgca gccggacgac agctttttga tgtttcgcgc
    19861 cagggaaaag ccagcgtcaa tgacgcggat cggctacgca aatacctggc gcgttttggt
    19921 ctgacgtggg aagccgtgca ggatcagcac agctccagtt gaatatgatg gtccgtcagc
    19981 acctgcatta cgcttactgg cggcggggcg tcggtgtaga cggcatctac catgctgatg
    20041 ctgcccatat tgaccatcgc gttacggcca aatttcgagt gatcgacaac cagcataacg
    20101 tggcgcgagt tctcaataat ggcgcgcttg gtgcgaactt cgtggtaatc gaactccagc
    20161 agcgagccgt cgctatcgat gccgcttatc cccagaatgc cgaaatcaag gcggaactgg
    20221 gagataaaat cgagcgtcgc ttcgccaatg atcccgccat cgcggctgcg taattcgcca
    20281 ccagcgagaa taatgcggaa atcttctttt accatcaacg tgttagcaac attgagattg
    20341 ttggtgacaa tacgcaaatt gctgtgatta agcagcgcgt gcgccaccgc ttccggcgtg
    20401 gtgccgatat cgataaacag tgtcgagccg ttggggattt gctccgccac tttgcgggca
    20461 atcctctctt tttcttcggt ctgggtggct ttgcgatcgt gccacggcgt gttaaccgaa
    20521 ctggaaggca gcgccgcacc gccatggtga cgcaggatca ggttttgctc cgccagctca
    20581 ttgaggtcgc ggcgaatagt ctgcgggctg acggagaaat gctctaccag ctcttcagta
    20641 ctgacataac cctgctgttt aaccagttcg ataataccgt tgtgacgttg tgtttgtttc
    20701 atttataaat ccctggaatt attttcgttt tcgcgcattg agcgaatcaa caaaagccat
    20761 cgctaaaccc acggctaacc cggcgatgtg tgctccgttc gccatcgaca tcccaaacaa
    20821 atcaaaccat ccggcgataa tccagatcag cgcaaagata attaacccac gttgcaggta
    20881 aatgccactt tgcggatcgc gctcaccacg tagccagacg tagcccatca gcgcatacac
    20941 cacgccagaa agcccgccaa accacggccc gctgaatttt tgctgcacat agccgcttaa
    21001 cagggcacta atgagagtaa taacaattag cttaccgcta ccgaggcgtt tttccaccgc
    21061 accgccgaga taccaccacc agagcaggtt aaagaggata tgcatcagcg agaagtgcat
    21121 taacgcgtgg gtgaagtaac gccagaactc aaatttcagt gttggatcga atggccaggc
    21181 cagccataac atcacttcct gatcgccgag aatttgcatg gcgataaaca ccaccacgca
    21241 ggcgatcatc accacccagg ttaccggacc tgcgcgttca cgcaaggcag caaagaaagg
    21301 ataacggcga taatgcagac cactgccggt atggcccgcc tgccagctgg ccgccagata
    21361 acgcggatct gccgggtttt cgagaaaacg cgccagctcc gcccgcacgc gctcggcctg
    21421 ggactcatcc gccagccaga catcgctttg gttatgttgt tgaatcgtaa ggataacacc
    21481 ctgcgtcgcc atgtaatcaa caaacgcctg cgccacgcgg gggttagcaa aagaggtaat
    21541 catcaacatc gttgctgtcg cttattccac acaaaagggg acagtataaa gcgttacgcg
    21601 ccgtacgcca cctctgcggg aaactgacgc tgccaggctt caaaaccgcc gtcaatgcta
    21661 tagaccacat cgtagccctg ttgcagcaga tactgcgccg cgcctttgct gctattgccg
    21721 tgataacaca tcaccatcac cggagtgtca aagtcgttat cacgcataaa cgcgcccagc
    21781 gtgtcgttgg ttaaatggaa agcctgcacc gcatgtccca ttgcgaaact ctgtggatcg
    21841 cgaatatcga ccagcaccgc ctctttttcc tgcaacttct ggtgcgcgtc ggcaacgtta
    21901 atacattcga acagatccat gcgtctctct ttctttacaa acaagtgggc aaatttaccg
    21961 cacagtttac gtcgaagcgg cagataaacg ccataatgtt atacatatca ctctaaaatg
    22021 ttttttcaat gttacctaaa gcgcgattct ttgctaatat gttcgataac gaacatttat
    22081 gagctttaac gaaagtgaat gagggcagca tggaaaccaa agatctgatt gtgataggtg
    22141 gcggcatcaa tggtgctggt atcgcggcag acgccgctgg acgcggttta tccgtgctga
    22201 tgctggaggc gcaggatctc gcttgcgcga cctcttccgc cagttcaaaa ctcattcacg
    22261 gtggcctgcg ctaccttgag cactatgaat tccgcctggt cagcgaggcg ctggctgaac
    22321 gtgaagtgct gctgaaaatg gctccgcata tcgccttccc aatgcgtttt cgcctgccac
    22381 atcgtccgca tctgcgcccg gcgtggatga ttcggattgg tctgtttatg tacgatcatc
    22441 tgggtaaacg caccagcctg ccgggatcaa ccggtttgcg ttttggcgca aactcagtgt
    22501 taaaaccgga aattaagcgc ggattcgaat attctgactg ttgggtagac gacgcccgtc
    22561 tggtactcgc caacgcccag atggtggtgc gtaaaggcgg cgaagtgctt actcggactc
    22621 gcgccacctc tgctcgccgc gaaaacggcc tgtggattgt ggaagcggaa gatatcgata
    22681 ccggcaaaaa atatagctgg caagcgcgcg gcctggttaa cgccaccggc ccgtgggtga
    22741 aacagttctt cgacgagggg atgcatctgc cttcgcctta tggcattcgc ctgatcaaag
    22801 gcagccatat tgtggtgccg cgtgtgcata cccagaagca ggcctacatt ctgcaaaacg
    22861 aagataaacg tattgtgttc gtgatcccgt ggatggacga attttccatc atcggcacca
    22921 ccgacgtaga gtataaaggc gatccgaaag cggtgaagat tgaagagagt gaaatcaact
    22981 atctgctgaa agtgtataac gcgcacttta aaaaacagct tagccgtgac gatatcgtct
    23041 ggacctactc cggtgtgcgt ccgctgtgtg atgatgagtc cgactcgccg caggctatta
    23101 cccgtgatta cacccttgat atccatgatg aaaacggcaa agcaccgctg ctgtcggtat
    23161 tcggcggtaa gctgaccacc taccgaaaac tggcagaaca tgcgctggaa aaactgacgc
    23221 cgtattatca gggtattggc ccggcgtgga cgaaagagag cgtgctgccg ggtggtgcca
    23281 ttgaaggcga ccgcgacg
//
LOCUS       DAJTOR010000051        23033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTOR010000051 DAJTOR010000000
VERSION     DAJTOR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.051"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..565)
                     /locus_tag="OO249_001782"
     CDS             complement(<1..565)
                     /locus_tag="OO249_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5636111.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGH"
     gene            complement(784..1761)
                     /gene="epmA"
                     /locus_tag="OO249_001783"
     CDS             complement(784..1761)
                     /gene="epmA"
                     /locus_tag="OO249_001783"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5636112.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2086..3894
                     /gene="frdA"
                     /locus_tag="OO249_001784"
     CDS             2086..3894
                     /gene="frdA"
                     /locus_tag="OO249_001784"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5636113.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3887..4621
                     /gene="frdB"
                     /locus_tag="OO249_001785"
     CDS             3887..4621
                     /gene="frdB"
                     /locus_tag="OO249_001785"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5636114.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4632..5027
                     /gene="frdC"
                     /locus_tag="OO249_001786"
     CDS             4632..5027
                     /gene="frdC"
                     /locus_tag="OO249_001786"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5636115.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5038..5397
                     /gene="frdD"
                     /locus_tag="OO249_001787"
     CDS             5038..5397
                     /gene="frdD"
                     /locus_tag="OO249_001787"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5636116.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5460..6593
                     /locus_tag="OO249_001788"
     CDS             5460..6593
                     /locus_tag="OO249_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5636117.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6682..7215
                     /gene="blc"
                     /locus_tag="OO249_001789"
     CDS             6682..7215
                     /gene="blc"
                     /locus_tag="OO249_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5636118.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7212..7529)
                     /gene="sugE"
                     /locus_tag="OO249_001790"
     CDS             complement(7212..7529)
                     /gene="sugE"
                     /locus_tag="OO249_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5636119.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESAHPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7711..7857)
                     /gene="ecnB"
                     /locus_tag="OO249_001791"
     CDS             complement(7711..7857)
                     /gene="ecnB"
                     /locus_tag="OO249_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5636120.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7968..8093)
                     /gene="ecnA"
                     /locus_tag="OO249_001792"
     CDS             complement(7968..8093)
                     /gene="ecnA"
                     /locus_tag="OO249_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5636121.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8145..8711)
                     /gene="efp"
                     /locus_tag="OO249_001793"
     CDS             complement(8145..8711)
                     /gene="efp"
                     /locus_tag="OO249_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5636122.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8753..9802
                     /gene="epmB"
                     /locus_tag="OO249_001794"
     CDS             8753..9802
                     /gene="epmB"
                     /locus_tag="OO249_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5636123.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            10176..11045
                     /locus_tag="OO249_001795"
     CDS             10176..11045
                     /locus_tag="OO249_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5636124.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(11248..11601)
                     /locus_tag="OO249_001796"
     CDS             complement(11248..11601)
                     /locus_tag="OO249_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5636125.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11739..13385)
                     /gene="groL"
                     /locus_tag="OO249_001797"
     CDS             complement(11739..13385)
                     /gene="groL"
                     /locus_tag="OO249_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5636126.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13429..13722)
                     /locus_tag="OO249_001798"
     CDS             complement(13429..13722)
                     /locus_tag="OO249_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5636127.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13998..15254
                     /gene="yjeH"
                     /locus_tag="OO249_001799"
     CDS             13998..15254
                     /gene="yjeH"
                     /locus_tag="OO249_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5636128.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPCAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRSASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGIIT"
     gene            complement(15270..15746)
                     /gene="fxsA"
                     /locus_tag="OO249_001800"
     CDS             complement(15270..15746)
                     /gene="fxsA"
                     /locus_tag="OO249_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5636129.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            16083..17519
                     /gene="aspA"
                     /locus_tag="OO249_001801"
     CDS             16083..17519
                     /gene="aspA"
                     /locus_tag="OO249_001801"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5636130.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17637..18938
                     /gene="dcuA"
                     /locus_tag="OO249_001802"
     CDS             17637..18938
                     /gene="dcuA"
                     /locus_tag="OO249_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5636131.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            19054..19392
                     /gene="cutA"
                     /locus_tag="OO249_001803"
     CDS             19054..19392
                     /gene="cutA"
                     /locus_tag="OO249_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5636132.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19368..21065
                     /gene="dsbD"
                     /locus_tag="OO249_001804"
     CDS             19368..21065
                     /gene="dsbD"
                     /locus_tag="OO249_001804"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5636133.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNHALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            21102..21677
                     /locus_tag="OO249_001805"
     CDS             21102..21677
                     /locus_tag="OO249_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5636134.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKQLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21784..21859
                     /locus_tag="OO249_001806"
     tRNA            21784..21859
                     /locus_tag="OO249_001806"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21817..21819,aa:Phe,seq:gaa)
     gene            22057..>23033
                     /locus_tag="OO249_001807"
     CDS             22057..>23033
                     /locus_tag="OO249_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5636135.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGTGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQTVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENVMNAS"
BASE COUNT         5740 a         5945 c         5701 g         5647 t
ORIGIN      
        1 aatgcccacc atttaataac aaaatagtaa tgcttactaa cagcaatact aaattcagac
       61 acattactgc aataccgccc accgcagtaa tgcgggcaat tttattaatc ccctttgaag
      121 caacgacggt gaccagaatc atccatgcta ctgccagcag accaaccacc tgcgtaggct
      181 ccagtccggc aatacgccag tgctgggtca tgtcgctacc atagaggaat gttgagaacg
      241 gtacccaaac tttcgcggag gtactcacca tccagatgat ataagaggaa aaccacataa
      301 acgtaccaat gaaggcaaaa cgtggtccga cactattatt catccaggaa tagataccac
      361 cttcttcttt gcgataagca gctcccattt cagccatcat taaggcgaat ggaataaaga
      421 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      481 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      541 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      601 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      661 atacccggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      721 catatgctac agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      781 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      841 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      901 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      961 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
     1021 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
     1081 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
     1141 ttctttgcca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
     1201 gtcttcttcg gtatcagcaa cgttactcaa atccagtttc gctgcgactt cccgcagttg
     1261 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
     1321 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1381 caaccggtac atatcatagt gcggtcgata ccactcaagc atagtgaact cagggttgtg
     1441 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1501 ggcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1561 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1621 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcag caaagaaacg
     1681 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1741 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1801 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1861 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1921 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1981 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     2041 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     2101 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     2161 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     2221 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     2281 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     2341 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtgggggtg cccatggagc
     2401 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2461 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2521 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2581 ggtcatgttc gcggcctggt agcaatgaac atgatggaag gcacgctggt gcagatccgt
     2641 gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg tttatcgtta caacaccaac
     2701 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2761 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2821 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2881 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2941 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     3001 tccacgccgc gtggcgatgt ggtttatctc gacttgcgtc acctcggcga gaaaaaactg
     3061 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     3121 aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat cgaaaccgat
     3181 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     3241 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     3301 atggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagaggca
     3361 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa ccaggatggc
     3421 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctag caatggaaga aggttgcggt
     3481 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3541 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3601 accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3661 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3721 gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3781 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3841 gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa
     3901 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3961 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     4021 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     4081 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     4141 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     4201 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     4261 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     4321 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4381 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4441 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg
     4501 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4561 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4621 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4681 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4741 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggacgcct
     4801 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4861 tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4921 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4981 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     5041 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     5101 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     5161 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     5221 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     5281 cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc ctgcgggcaa atgggttttc
     5341 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtatcgttac aatctaacgt
     5401 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5461 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca tttgctgccc
     5521 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5581 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5641 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5701 tcagcaaaac atttacgggc gtgcttggtg gcgatgctat tgctcgaggg gaaatcaagt
     5761 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5821 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcagatg ccggatgaag
     5881 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5941 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     6001 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     6061 accatacgtg gattaatgta ccgcccgcag aagaaaagaa ttacgcctgg ggatatcgcg
     6121 aaggcaaggc agttcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     6181 cgaccattga agatatggcc cgctgggtgc aaagcaattt aacccccctt gatatcaatg
     6241 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     6301 tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta
     6361 acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa
     6421 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
     6481 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     6541 ccaatccagc gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca
     6601 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
     6661 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
     6721 tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtgacagt ggtgaataat
     6781 ttcgacgcta aacgctatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
     6841 gaacgtggac tggaaaaagt caccgcaaca tacagtctgc gtgatggcgg cggcctgaat
     6901 gtcatcaata aaggctataa ccctgacaga gaaatgtggc agcagagtga agggaaagcg
     6961 tactttaccg gcgcaccaac acgcgctgcg ctgaaagtgt cattctttgg tcctttctat
     7021 ggcggttata acgtcattgc acttgatcgg gaataccgcc atgcgctggt ttgcggcccg
     7081 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
     7141 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
     7201 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
     7261 agactcgcca ggcgcatcgg gtgagcggac tcaccgagca gcacaatgcc ggtgatagcc
     7321 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctactgg taacgatttc
     7381 atcgcccagg caagtagcgc catactgaca atcatcgccg tcacggtaat aacactcggc
     7441 gtcaaacgac taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
     7501 ccagcaataa ctaagataat ccaggacata tcaggctccg gagaattggg gccgtccccg
     7561 gtgaaaagac gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
     7621 ggtggcaggg gaatttcaac tattacctta gtgactatcg cagaaaaaac aaacggcacg
     7681 acacagctgt cgtaccgtta ttgcttattg ctattgctgc gctttcgttg ctgcgccaga
     7741 aatcgcgtta ccgccatcag aaatgtcttc accaacgcca cgcgtggtgt tgcaggcagt
     7801 taatactgtt gaaagcacca gaacagaaaa gatcgctgca attgtcttct tcaccataac
     7861 gtcttccttt taggtaatgt tatttatgtt tgcctatagc aaattaataa tagacaacat
     7921 cgccaaaaat gacggaaatc tgatgatttt tcggaagaga agaaaaatta gctggcagcg
     7981 cgagagatgg agttgccgag atgtttgatg tcttcgccga aaccgcgagc ggtgttacag
     8041 cccgtgagca gcgtactggc aagcaaaaca agaacgataa ggcgtttcat catctctgac
     8101 ctgaataagt gatggtgcag cctgcaggcc gcaccacaac cgcattactt cacgcgagag
     8161 acgtattcac cagagcgggt atccactttg atgacttcgc cgatttgtac gaacagcgga
     8221 accttaacca cagcgccagt agacagggta gccggtttgc cgccagtacc tgcggtatca
     8281 cctttcaggc ccggatcggt atcaacgatt tccagttcaa cgaagttcgg cggagtaacg
     8341 gagatcggct gaccattcca cagagttacg atacactctg cctgatccag cagccattta
     8401 gcgttatcgc caattgcttt tgcatcagca gacagctgct cgaaagtttc gttgttcatg
     8461 aagtgccaga actcaccgtc gttgtacagg taagtcaggt tcatatcgac aacatcagcg
     8521 ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc gagtaccggt cagcagacga
     8581 cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt ttacgaattc actcgcttca
     8641 accgcgtaag gttcgccgtc taacatgatt ttaagaccag cacgaaaatc gttgctatag
     8701 tacgttgcca taaggccctc tgaaatttgt taattggtag ctaagccaca aaatggcgca
     8761 tattgtaacc ctaaataccc catccagaga agattggtta acgcaacttg ccgatgttgt
     8821 gaccgatcct gatgaacttc tgcgtctttt gaatatagac gcggacgaaa aactgttggc
     8881 cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc cgctcattta tcgatcgcat
     8941 ggagaaaggc aatccggacg atcctctttt gcgtcaggta cttacctcgc aagatgagtt
     9001 tgtcgtcgcg tccggattct ccaccgaccc actggaagaa cagcacagcg tagtgcctgg
     9061 tttgttgcat aaataccaca accgggcgct tttgctggtc aaaggcggct gcgcggtaaa
     9121 ttgccgctat tgcttccgtc gccacttccc ctatgccgaa aatcagggca acaagcgtaa
     9181 ctggcagact gcacttgagt atgttgctgc gcatccggaa ctggacgaga tgattttctc
     9241 cggcggcgat ccgctgatgg cgaaagatca cgagctggac tggttgctca cacaactgga
     9301 agccatcccg catatcaaac gtctgcggat tcacagccgt ctgccgattg tgatcccggc
     9361 acgcatcaca gatgcgctgg ttgaacgctt ttcacattct acgctgcaaa tcctgctggt
     9421 gaatcacatc aaccatgcca atgagataga tgaaacattc cgtcaggcga tggctaagtt
     9481 gcgccgggta ggcgttactt tgctgaacca gagcgttctg ttacgtgatg tgaacgataa
     9541 cgcacaaacg ctggcaaacc tgagtaatgc gttgttcgat gccggcgtga tgccgtatta
     9601 cctgcatgtg ctcgataaag tacagggcgc agcgcatttt atggtgagtg atgacgaagc
     9661 acggcagatt atgcgtgagt tgctgacatt ggtgtcggga tatctggtgc cgaaactggc
     9721 gcgagaaatt ggcggcgaac ccagcaaaac gccgctggat ctccagctac gccagcagtt
     9781 actgattaaa cagggaagtt aaacattaaa attgcctgat gcgctacgct tatcaggcct
     9841 acatcgtgca ttgaaattga atggatttgc aatgaactgt aggccggata aggcgtttac
     9901 gccgcatccg gcaagagcaa caaacgcaag tacagcaacc tgtcattaaa ctgagaaatt
     9961 taatctttgc ccgttgcata acctttaatt attcatatgc attgctgttg ttacttttta
    10021 tatagctaac accaataatt aacctttctt ttctgtttat atagagtgag cacggctatt
    10081 tatgttattt taacaactat tactaccaag cccatttatc tggcttataa attaaaatta
    10141 attatttaat tgagaaatgg ttagggagaa cctacatggc cataagcatc aagggtgtta
    10201 ataccggtgt tattcgcaag agtaataatt tcattgcact ggcgctgaaa attaaagaac
    10261 ctcgcaataa agaatcgttg ttctttatgt ctgtcatgga actgcgcgac ctgctgattg
    10321 cgctggaaag tcgtctgcat caaaagcata aactggatgc tgctgcacgc cttcagtatg
    10381 agcaagctcg cgataaagtc attaagaaaa tggcagaaaa catcccagaa attctggttg
    10441 atgagcttaa aaatgccgat attaatcgtc gggttaacac gctggaactt accgataatc
    10501 aaggcgaaaa tcttaccttc gtattaacgc tacatgacgg tagcaaatgt gagttagtcg
    10561 tcaatgagtt gcagattgaa atgctagcgc gagcaatcat tcatgcaatt aataatgcgg
    10621 aaatgcgtga actggcatta cgtattacct cactgttaga tttcctgccg ttatatgacg
    10681 ttgactgcca ggaaaatggc aatctggaat atgacaccta ttcccaaccg gaatggaaac
    10741 ataacttgtt cgaccattat ctggcggtgc tttaccgctt taaagatgaa agtggcaaag
    10801 aacaatttag cggcgcagtg gtgaaaacgc gtgaagcaac gccgggtaaa gaagtagagg
    10861 cgatcactcg ccgaatgctt gatttcagcc cacgactgaa aaaactcgcg ggtgtgccat
    10921 gtcaggtcta tgttcgcacc gtagcggcga ataacgccca accgctaacc caggatcaat
    10981 gcttacgcgc gctgcatcac ttacgtgttc aatccaccag caaaactgca ccacaggcga
    11041 aataatctca tctttgctaa tgcgaggctg gagccatcca gcctgaaatg actgataacg
    11101 tgatcgttgg tcatgccgga tgcggcgtaa acgccttatc cggcctacaa attctcaagc
    11161 attcaatcag ttgcaatgcc tgctgtaggc ctgataagcg aagcgcatca ggcaataata
    11221 cgtttgtcac ccagcctctg gatcacatca gttcgggcac ttataaacct gaccgataat
    11281 ctggctatcc gtcgggacaa aactggacaa cattccctgc gacgggctgc tgatgccata
    11341 aatcacgtta ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg cgccgcgcat
    11401 agaaccgccc tcttctccgt gttgcccgga aagccagttg ctttgcttac ctgtcgcagt
    11461 accaatcagc tggcactctg cgcctggctg ctcgtccaca atgcgtacac tctgaccggc
    11521 agcactcaat tcgttgctgg agctacaacc cgccatcagt agcgcggcac cgacaatccc
    11581 tgctaagtat tttacgtgca tgttattccc cataatcaat gagctggacg ccactcgcgt
    11641 cgtccgtgtc tgaatcttat actagaaaga tgaccaaaag aaaaaccccc agacatttct
    11701 gcccgggggt ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc gcccatgcca
    11761 cccatgccgc ccataccgcc agcagcgcct aagtcagctg catcgttttt cggcaggtcg
    11821 gtaaccatgc actcggtggt gatcatcagg ccagccacag aagccgcgta ctgcagagca
    11881 gaacgggtta ctttggttgg atccaagata cccatgtcga tcatgttgcc gtattcttcg
    11941 gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa cagtgttagc aacaacagac
    12001 ggttcttcgc cgcagttcaa tacgatctga cgcagcggag cttccattgc acgcagtgca
    12061 actttgatac ccacgttctg gtcttcgttc tgaccacgca ggtcagccag tttagacgct
    12121 acgcggatca gcgcaacacc accaccagca accacgcctt cttctaccgc agcacgggtc
    12181 gcgtgcaggg catcttcaac gcgtgctttt ttctctttca tttcaacttc ggtagcagca
    12241 cctactttga taactgcaac gccgcctgcc agtttcgcta cgcgctcctg cagtttttca
    12301 cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga tctgagcaac acggccctgg
    12361 attgcagctt cttcacccac gccatcaatg atggtggtgg tgtctttgtt gatcacaaca
    12421 cgtttagcct gacccaggtc ttccagggtt gctttttcca gctccatacc gatctcttca
    12481 gagattacgg taccgccagt cagggttgcg atatcctgca gcatagcttt acggcgatcg
    12541 ccgaagcccg gagctttaac cgcagcaact ttcacgatgc cacgcatggt gttaacaacc
    12601 agagttgcca gcgcttcgcc ttcaacatct tcagcgatga tcagcagcgg tttacctgct
    12661 ttagcaacgg cttccagaac cggcagcatt tcgcggatgt tggagatttt cttgtcagcc
    12721 agcaggatga acgggctttc cagttctact gcgccagttt ccggcttgtt gatgaagtaa
    12781 ggagacaggt agccacggtc gaactgcata ccttcaacca cgtccagttc gtcctgcaga
    12841 ccggtaccgt cttcaacggt gataacgcct tctttaccga ctttgtccat cgcttcagcg
    12901 atcagtttac ctacggtttc gtcggagtta gcggagatgg taccaacctg agcaatcgct
    12961 ttagagtcag agcatggtac ggacagcgct ttcagttctt caactgcagc ggtaacagct
    13021 ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg cagcaactgc tttcagaccc
    13081 tcagtgatga tagcctgagc cagtacggtt gcagtggtgg taccgtcgcc tgcagcgtcg
    13141 ttcgctttag aggcaacttc tttcaccatc tgcgcaccca tattttcgaa cttgtcttcc
    13201 agttcgattt cacgagcaac ggaaacacca tctttggtga tggtcggtgc accgaaagat
    13261 ttatccagaa ctacgttacg gcctttcgga ccgagggtaa ctttcactgc atctgccagt
    13321 acgtttacgc cgcgcagcat tttcacacga gcgtcgttac cgaattttac gtctttagct
    13381 gccattatct ttattcctta aattcgtatg ttcagtgtcg tgcgaggatt acgcttcaac
    13441 aattgccaga atgtcgcttt cggacatgat caacacttct tcattgtcga tcttctcaga
    13501 tttcacaccg tagccatcgt tgaaaataac gatgtcgcca actttcacat ccagcggctt
    13561 cacttcgcca ttttcaagga tacggccatt gccgacagcc agcacttcgc cgcgggtgga
    13621 tttagccgct gcagagccgg tcagaacgat gccgccagca gatttagttt caacttcttt
    13681 acgcttgacg atcacgcgat catgcaatgg acgaatattc attgataact ctcctttgag
    13741 aaagtccgta tctgttatgg gtgacgccgg agcttacgtg gtttcccggc tggtgaccag
    13801 agaaatgggg atgaggattc gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc
    13861 acaaaatcgg gtgaaaaccc tgattcacct cacatttcat cgcaatttct tcatcgccgt
    13921 cataagcgaa tctgattgtg ctaccatcga aaatctacgc agttgcccaa aatttgggcg
    13981 caatcggata tcaacccatg agtggactca aacaagaact ggggctggcc cagggcattg
    14041 gcctgctatc gacgtcatta ttaggcaccg gcgtgtttgc cgttcctgcg ttagctgcac
    14101 aggtagcagg caataacagc ctgtgggcgt ggcccgtttt gattatctta gtgttcccga
    14161 ttgcgattgt gtttgcgatt ctgggtcgcc actatccctg cgcaggcggt gtcgcgcact
    14221 tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac cggctggttg tttttatcgg
    14281 tcattcccgt gggtttgcca gccgcgctac aaattgccgc cgggttcggc caggcaatgt
    14341 ttggctggca tagctggcaa ctgttgttgg cagaactcgg tacgctggcg ctggtgtggt
    14401 atatcggtac tcgcagtgcc agttccagtg ctaatctaca aaccgttatt gccggactta
    14461 tcgtcgcgct gattgtcgct atctggtggg cgggcgatat caaacctgcg aatatcccct
    14521 tcccggcacc tggtaatatc gaacttaccg ggttatttgc tgcgttatca gtgatgttct
    14581 ggtgttttgt cggtctggaa gcatttgccc atcttgcctc ggaatttaaa aatccagagc
    14641 gtgattttcc tcgcgcattg atgattggtc tgctgctggc aggattagtc tactggggct
    14701 gtacggtagt cgtcttacac ttcgacgcct atggtgaaaa aatggcggcg gcagcatcgc
    14761 ttccaaaaat tgtagtgcaa ctgttcggtg taggagcgtt atggattgcc tgcgtgattg
    14821 gctatctggc ctgctttgcc agtctcaaca tttatataca gagcttcgcc cgcctggtct
    14881 ggtcgcaggc gcaacataat cctgaccact acctggcacg cctctcttct cgccatatcc
    14941 cgaataatgc cctcaatgcg gtgctcggct gctgtgtggt gagcactttg gtgattcatg
    15001 ctttagagat caatctggac gctcttatta tttatgccaa tggcatcttt attatgattt
    15061 atctgttatg catgctggca ggctgtaaat tattgcaagg acgttatcga ctactggcgg
    15121 tggttggcgg gctgttatgc gttctgttac tggcaatggt cggctggaaa agtctctatg
    15181 cgctgatcgt gctggcgggg ttatggctgt tgctgccaaa acgaaaaacg ccggaaaatg
    15241 gcataatcac ataatccggc gtttcgacat taatcctggc gatcgtcttt atgatcaagg
    15301 cggtcgcgct catcatcctt tcgctggtac tcaccatcaa aagtattacc gccaccggtc
    15361 ccggcgctaa aaccgccgcc tggcatacga gaaaagcgca aatgcggcat caacttcact
    15421 gtcagatgct tttgcaccgg cggtaataaa agcagaagac cgaggaagtc ggtgaaaaag
    15481 cccggtaata aaagcagcaa accggcaatg atcagcgaaa cacttttaat catctccgcc
    15541 gctgggtttt cacccgccgc cattttttgc tgcatcagca cgaaattctt aaagccctgg
    15601 ttacgtacca gtgacatccc gataactgac gtgaatataa ccagcacgag ggtcagcaat
    15661 acccccaata catgggcaac ctgaataaag attgaaatct caatatagac ataaaggaaa
    15721 atggcaataa aaggtaacca gcgcaaaggt ttctcctgta atagcagccg gtaaaacccg
    15781 gctacctgaa tgggttgcga atcgcgttca gcttatattg tggtcattag caaaatttca
    15841 agatgtttgc gcaactattt ttggtagtaa tcccaaagcg gtgatctatt tcacaaatta
    15901 ataattaagg ggtaaaaacc gacacttaaa gtgatccaga ttacggtaga aatcctcaag
    15961 cagcatatga tctcgggtat tcggtcgatg caggggataa tcgtcggtcg aaaaacattc
    16021 gaaaccacat atattctgtg tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc
    16081 acatgtcaaa caacattcgt atcgaagaag atctgttggg taccagggaa gttccagctg
    16141 atgcctacta tggtgttcac actctgagag cgattgaaaa cttctatatc agcaacaaca
    16201 aaatcagtga tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcta
    16261 tggcaaacaa agagctgcaa accattccta aaagtgtagc gaatgccatc attgccgcat
    16321 gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt cccggtagac gtctaccagg
    16381 gcggcgcagg tacttccgta aacatgaaca ccaacgaagt gctggccaat atcggtctgg
    16441 aactgatggg tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac catgttaaca
    16501 aatgtcagtc cactaacgac gcctacccga ccggtttccg tatcgcagtt tactcttccc
    16561 tgattaagct ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg
    16621 aattccagga catcctgaaa atgggtcgta cccagctgca ggacgcagta ccgatgaccc
    16681 tcggtcagga attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa aatatccaac
    16741 gtaccgctga actgctgctg gaagttaacc ttggcgcaac agcaatcggt actggtctga
    16801 acacgccgaa agagtactct ccgctggcag tgaaaaaact ggctgaagtc actggcttcc
    16861 catgcgtacc ggctgaagac ctgatcgaag cgacctctga ctgcggcgct tatgttatgg
    16921 ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa aatctgtaac gacctgcgct
    16981 tgctctcttc tggcccacgt gccggcctga acgagatcaa cctgccggaa ctgcaggcgg
    17041 gctcttccat catgccagct aaagtaaacc cggttgttcc ggaagtggtt aaccaggtat
    17101 gcttcaaagt catcggtaac gacaccactg ttaccatggc agcagaagca ggtcagctgc
    17161 agttgaacgt tatggagccg gtcattggcc aggccatgtt cgaatccgtt cacattctga
    17221 ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg catcactgct aacaaagaag
    17281 tgtgcgaagg ttacgtttac aactctatcg gtatcgttac ttacctgaac ccgttcatcg
    17341 gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg
    17401 aagtcgttct ggaacgcggt ctgttgactg aagcggaact tgacgatatt ttctccgtac
    17461 agaatctgat gcacccggct tacaaagcaa aacgctatac tgatgaaagc gaacagtaat
    17521 cgtacagggt agtacaaata aagaaggcac gtcagatgac gtgccttttt tcttgtgaac
    17581 agtaacttaa aaacaacaat ctaatatcaa cttgttaaaa aacaaggaag gctaatatgc
    17641 tagttgtaga actcatcata gttttgctgg cgatcttctt gggcgccaga ttggggggaa
    17701 taggtattgg ttttgcaggc ggattggggg tgctggttct tgccgctatt ggcgttaaac
    17761 ccggtaacat cccgttcgat gtcatttcca ttatcatggc ggttatcgcc gctatttctg
    17821 ccatgcaggt tgctggcggt ctggactatc tggttcatca gacagaaaag ctgctgcgcc
    17881 gtaacccgaa atacatcacg atcctcgcac ctatcgtgac ctatttcctg acaatctttg
    17941 ctggtactgg caacatctct ctggcgacac tgccagttat cgctgaagtt gcgaaggaac
    18001 aaggcgttaa accttgccgt ccgctctcta ctgcagtggt atccgcgcag attgcgatca
    18061 ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc cgtgatggaa ggtcatggca
    18121 tcagctacct ccatctgctc tccgtggtca tcccgtccac cctgctggcg gttctggtga
    18181 tgtcctttct ggtcactatg ctgttcaact ccaaactctc tgacgatccg atttatcgca
    18241 agcgtctgga agagggcctg gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg
    18301 gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt atctatgcaa
    18361 tcatcaacag cccaagcatg ggtctggttg aaaaaccgct gatgaacacc accaacgcaa
    18421 tcctgatcat catgctcagc gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg
    18481 acaacatcct caactccagc accttcaaag caggtatgag cgcctgtatt tgtatcctgg
    18541 gtgttgcgtg gctgggcgat actttcgttt ccaacaacat cgactggatc aaagataccg
    18601 ctggtgaagt gattcagggt catccgtggc tgctggccgt catcttcttc tttgcttctg
    18661 ctctgctgta ctctcaggct gcaaccgcaa aagcactgat gccgatggct ctggcactga
    18721 acgtttcacc gctgaccgct gttgcttctt tcgctgcggt gtctggtctg ttcattctgc
    18781 cgacctaccc gacgctggtt gctgcggtac agatggatga cacgggtact acccgtatcg
    18841 gtaaattcgt cttcaaccat ccgttcttca tcccgggtac tctgggtgtt gccctggccg
    18901 tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg atcaatcgcg gggcgttcac
    18961 gccccgcttt ctttcccgcc gactaacatc cttcccccgt acgttgtata gtgacctctc
    19021 tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag ttcgaatacc
    19081 gcgtctgtcg tggtgctatg tacggcacca gatgaagcga cagcccagga tttagctgcc
    19141 aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga tccccggcgc tacctctctc
    19201 tattactggg aaggtaagct ggagcaagaa tacgaagtgc agatgatttt aaaaactacc
    19261 gtatctcacc agcaggcact gctggaatgc ctgaagtctc atcatccata tcaaaccccg
    19321 gaacttctgg ttttacctgt tacacacgga gacacagatt acctctcatg gctcaacgca
    19381 tctttacgct gatcctgcta ctttgcagca cttccgtttt tgccggatta ttcgacgcgc
    19441 cgggacgttc acaatttgtc cccgcggatc aagcctttac ttttgatttt cagcaaaacc
    19501 aacatgacct taatctgacc tggcagatca aagacggtta ctacctctac cgtaaacaga
    19561 tccgcattac gccggaacac gcgaaaattg ccgacgtgca gctgccgcaa ggcgtctggc
    19621 atgaagatga gttttacggc aaaagcgaga tttaccgcga tcggctgacg cttcccgtaa
    19681 ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac ctaccagggc tgtgctgatg
    19741 ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg gtcgccaaca
    19801 acgcagcgtc acagcctgtg tctgtttcgc agcaagagca gcacaccgcg caattgccct
    19861 tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca tgcgtgctgc
    19921 caatgtaccc actgatttct ggcatcgtgc tgggtggtaa acagcggctc tccactgcca
    19981 gagcattgtt gctgaccttt atttatgtgc aggggatggc actgacttac acggcgctgg
    20041 gtctggtagt tgccgccgca gggttacagt tccaggcggc gctacagcac ccatacgtgc
    20101 tcattggcct cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc ttgtttactc
    20161 tacaactccc ctcttcgctg caaacacgtc tcacgttgat gagcaatcgc caacagggcg
    20221 gctcacctgg cggtgtgttt gttatggggg cgattgccgg acttatctgt tcaccatgca
    20281 ccaccgcacc gcttagcgcg attctgctgt atatcgccca aagcgggaac atgtggctgg
    20341 gcggcggcac gctttatctc tatgcgttgg gcatgggcct gccgctgatg ctaattaccg
    20401 tctttggtaa ccgcttgctg ccgaaaagcg gcccgtggat ggaacaagtc aaaaccgcgt
    20461 ttggttttgt gatcctcgca ctgccggtct tcctgctgga gcgagtgatt ggtgatgtat
    20521 ggggattacg cttgtggtcg gcgctgggtg tcgcattctt tggctgggcc tttatcacca
    20581 gcctacaggc taaacgcggc tggatgcgcg tggtgcaaat aatcctgctg gcagcggcat
    20641 tggttagcgt gcgcccactt caggattggg catttggtgc gacgcatacc gcgcaaactc
    20701 agacgcatct caactttaca caaatcaaaa cggtagatga gttaaatcat gcgctcgttg
    20761 aagccaaagg caaaccggtg atgttagatc tttatgccga ctggtgcgtc gcctgtaaag
    20821 agtttgagaa atacaccttc agcgacccgc aggtgcaaaa agcgttagca gacacggtct
    20881 tacttcaggc caacatcacg gccaacgacg cacaagatgt ggcgctgtta aagcatctta
    20941 atgtccttgg cctaccgaca attctctttt ttgacggaca aggccaggag catccacaag
    21001 cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc acatttgcgc gatcgccaac
    21061 cgtgaacgac acttgcagcg ggaaagacgg aggaaaatag cgtgcaacgt gaagatgtac
    21121 tgggagaagc cctgaaatta ttagaattac aagggattgc caacaccacg ctggagatgg
    21181 ttgctgaacg tgtggattat ccactggacg agctacgccg cttctggcca gacaaagagg
    21241 caatcctcta cgatgcgctg cgttatctta gccaacagat agatgtctgg cgtcgtcagc
    21301 tgatgctgga cgaaacgcaa accgccgaac aaaagcagct ggcacgttat caggcgttat
    21361 cggagtgcgt taaaaacaac cgctatccgg gctgtctatt tatcgctgcc tgtacgtttt
    21421 atcccgatcc tggccaccct attcatcaac tggccgatca gcaaaaaagc gcggcctacg
    21481 atttcaccca cgaactgtta accacactgg aagttgacga tccggcgatg gtagcaaagc
    21541 agatggaact ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt agccaggcgg
    21601 atgtcgacac tgcacatcgg ctggcggaag atatcctgcg cttcgcccgc tgccgccagg
    21661 gtggtgcatt gacctgacag aaacacagaa aagaagcgat ttgccgcaat cttaagcagt
    21721 tgaatcgctt ttactgaaat taggttgacg agatgtgcag attacggttt aatgcgcccc
    21781 gttgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg
    21841 attccgagtc cgggcaccaa attcatatca acggacctct acggaggttc gtttttcgtt
    21901 tcagaacgcc acgatttaag cgttctgcct acaaatcaat tctaccgaac tcaaccagat
    21961 tccccccaca tcaaccccat tgtgtgggta taattgcggg cataccccag ttcgacagaa
    22021 tttgtaccct ctttaacgca ttcagaaggc tgaactatgg cactgactga cgcaaaaatc
    22081 cgggctgcaa agcccactga caaggcttat aaactcactg acgggactgg catgttcctg
    22141 ctggtacatc ctaatggctc ccgttactgg cgtctccgtt atcgtattct gggtaaggag
    22201 aagactctgg cacttggtgt gtatccagaa gtttctctct ccgaagctcg tacaaaacgg
    22261 gatgaggccc gaaaactgat ttcggagggg gttgaccctt gcgaacagaa aagagctaaa
    22321 aaagtagtcc ctgatttaca gctctctttt gaacatattg cgcgacgctg gcatgccagt
    22381 aataaacaat gggcacaatc acacagcgat aaagtactca aaagcctcga gactcacgtt
    22441 ttccccttta tcggcaaccg ggatatcaca acactcaata ccccggatct gcttatccct
    22501 gttcgtgctg cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
    22561 tctgccgtaa tgcgttatgc cgtacagtct ggcatcatca gatataatcc tgctctggat
    22621 atggctggcg cattgactac ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc
    22681 ctgcctgaac ttttgtcgcg tattggcagt tacaaagggc aaactgtcac ccagcttgcc
    22741 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct
    22801 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc cctgccaggc
    22861 gtaaaattct cacaccgggg ctccaagatg cgaacaccac atcttgtgcc actcagcaaa
    22921 caggctgtag ccatactgac agaactacag acatgggctg gtgaaaatgg tctgatcttt
    22981 acgggagcac atgacccgcg taaaccaatc agtgaaaatg taatgaacgc atc
//
LOCUS       DAJTOR010000052        22789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTOR010000052 DAJTOR010000000
VERSION     DAJTOR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.052"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            39..968
                     /gene="aaeR"
                     /locus_tag="OO249_001808"
     CDS             39..968
                     /gene="aaeR"
                     /locus_tag="OO249_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5636136.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1096..2541)
                     /gene="tldD"
                     /locus_tag="OO249_001809"
     CDS             complement(1096..2541)
                     /gene="tldD"
                     /locus_tag="OO249_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5636137.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2697..6497)
                     /gene="yhdP"
                     /locus_tag="OO249_001810"
     CDS             complement(2697..6497)
                     /gene="yhdP"
                     /locus_tag="OO249_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5636138.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFHLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6565..8034)
                     /gene="rng"
                     /locus_tag="OO249_001811"
     CDS             complement(6565..8034)
                     /gene="rng"
                     /locus_tag="OO249_001811"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5636139.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8024..8617)
                     /gene="yhdE"
                     /locus_tag="OO249_001812"
     CDS             complement(8024..8617)
                     /gene="yhdE"
                     /locus_tag="OO249_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5636140.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAHKKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8626..9114)
                     /gene="mreD"
                     /locus_tag="OO249_001813"
     CDS             complement(8626..9114)
                     /gene="mreD"
                     /locus_tag="OO249_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5636141.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9114..10199)
                     /gene="mreC"
                     /locus_tag="OO249_001814"
     CDS             complement(9114..10199)
                     /gene="mreC"
                     /locus_tag="OO249_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5636142.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPTTGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10265..11308)
                     /gene="mreB"
                     /locus_tag="OO249_001815"
     CDS             complement(10265..11308)
                     /gene="mreB"
                     /locus_tag="OO249_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5636143.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11613..13553)
                     /gene="csrD"
                     /locus_tag="OO249_001816"
     CDS             complement(11613..13553)
                     /gene="csrD"
                     /locus_tag="OO249_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5636144.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKYPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13705..14679
                     /locus_tag="OO249_001817"
     CDS             13705..14679
                     /locus_tag="OO249_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5636145.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVMVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15657..16127
                     /gene="accB"
                     /locus_tag="OO249_001818"
                     /gene_synonym="fabE"
     CDS             15657..16127
                     /gene="accB"
                     /locus_tag="OO249_001818"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5636146.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16138..17487
                     /gene="accC"
                     /locus_tag="OO249_001819"
     CDS             16138..17487
                     /gene="accC"
                     /locus_tag="OO249_001819"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5636147.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17579..18626)
                     /locus_tag="OO249_001820"
                     /pseudo
     CDS             complement(17579..18626)
                     /locus_tag="OO249_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(18623..19585)
                     /locus_tag="OO249_001821"
     CDS             complement(18623..19585)
                     /locus_tag="OO249_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5636148.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDVHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            complement(19607..20596)
                     /locus_tag="OO249_001822"
                     /pseudo
     CDS             complement(19607..20596)
                     /locus_tag="OO249_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(20597..22096)
                     /locus_tag="OO249_001823"
     CDS             complement(20597..22096)
                     /locus_tag="OO249_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5636149.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAERGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRITNPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(22157..>22789)
                     /locus_tag="OO249_001824"
     CDS             complement(22157..>22789)
                     /locus_tag="OO249_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5636150.1"
                     /translation="IISPVDSKGVQAAIIKADKAGIPVITVDVAAEGVPVVSHVATDN
                     YAGGVEAGKLMGKLLNGKGTVAIISYPALQSVVARVDGFKKGLSDTPDIKIVAEQPSI
                     TRAEALTTAQNIMQANANLNGLFGFGDDAALAAVIAAKSAHNDNIKIIGFDGMKEARD
                     AVDSEKTFAAVIRQYPDQMGAKAIDAAVDHLNGKPVEKMIPVQPGVYTGK"
BASE COUNT         5643 a         6179 c         5449 g         5518 t
ORIGIN      
        1 attatgtgat ctaaatcact tttaagtcag agtgaataat ggaacgacta aaacgcatgt
       61 cggtgtttgc caaagtagtt gaatttggct cttttaccgc cgccgccaga cagctacaga
      121 tgagcgtttc gtccatcagt cagacggtgt caaaactgga agatgagttg caggtaaagc
      181 tgttaaaccg tagcacacgc agcattggcc tgaccgaagc cggtagaatt tactaccagg
      241 gctgccgtcg tatgcttcat gaagtgcagg atgttcatga gcaactgtat gctttcaata
      301 acacccccat cgggacgcta cgcattggct gttcttcaac tatggcacaa aatgttctcg
      361 ccgggctgac agccaaaatg ctgaaagaat acccaggttt gagcgtcaat ctggttaccg
      421 gaattccagc ccccgacctg attgccgacg gtctggatgt ggtgatccgc gtcggcgcgt
      481 tgcaggattc cagcctgttt tcccgccgtc ttggcgcgat gccaatggtg gtgtgcgccg
      541 cgaaaagcta tctcacacaa tacggcatac cggaaaaacc cgccgattta agtagccatt
      601 catggcttga atacagcgtg cggcccgaca atgaatttga actgattgca ccggaaggga
      661 tctcgactcg cctgatcccg caagggagat ttgtgactaa tgatccgatg acgctggtgc
      721 gctggctaac ggcgggtgcc gggatcgcct acgtgccgct gatgtgggtg atcaacgaga
      781 tcaatcgtgg ggagctggag atcctgctgc cgcgttatca gtcagatcca cgcccggttt
      841 atgcgttata taccgaaaaa gataagctgc cgctgaaggt acaggtcgtg atcaactcgc
      901 tgacggatta ttttgttgag gtcggtaaat tgtttcagga gatgcacggg cgcgggaaag
      961 agaagtaatt catattgtac tgttacgttc tacaaacctg tgccaacggg ttcccctcac
     1021 cctaacccca gaggagcgag gggaccgttc gtgcacgtag aaagattaat tatccttctg
     1081 aaaataatga aattattacg cagtaccgcc aacagtcagg ttatcaactt tcagcgttgg
     1141 ctggcccacg ccaaccggca aactttgccc ttctttaccg cagacaccca cgccgttatc
     1201 cagtttcagg tcgttgccaa ccatcgaaat ctgctgcatg gtttcgatac cggaaccaat
     1261 caacgttgcg cctctcaccg gcttcgttac tttaccgttt tcaatcagat atgcttctga
     1321 agtggagaaa acgaatttgc cggaggtgat atccacctga ccgccaccaa agttcggtgc
     1381 atagataccg tactcaacgg attcaataat ttcctgcggg gtcgatttac ccggcagcat
     1441 ataggtgttg gtcatacgcg gcatgggcag atgggcgtag gattcacggc gaccgttgcc
     1501 agtcggcgtc atccccatca aacgcgcgtt gagtttatcc tgcatgtagc ctttcagaat
     1561 gccgttctca atcagcacgt tgtactggcc tggcgtacct tcgtcatcaa tcgccaccga
     1621 accacggcga tcgaccatcg tgccgtcatc aaccacggta cacagttctg aagccaccag
     1681 ctccccgacc tgcccactaa atactgaagt gccgcggcgg ttgaagtcgc cttccagacc
     1741 gtgacctacc gcttcatgca acagcacgcc cggccaacct gcgccaagta ctaccggcat
     1801 ggtgcccgct ggtgcagcaa cggcagaaag attgaccagc gccatacgca ctgcttcttt
     1861 tgcccatgca tccgcacgga cttcgccgtc aagatcggca aggaagaact cataaccaaa
     1921 acgaccgcca ccgccactgg caccgcgttc gcgtttgcca tcttcttcga ccagaacgct
     1981 cacggaaaga cgcaccagcg gacggacatc cgccgctagc gtgccgtcgg tggccgcaac
     2041 cagaattaat tcatagacac cactgaggct ggcagtcact tcctgtacgc gcttgtccgc
     2101 ttcgcgagca accttatcga cgcgacgcag gatatccagc ttctcttcac ggctcatgct
     2161 ttgcagcgga tctaccgagg tatacaacgg gctatgccct accgcgccca gcgtctgtac
     2221 tttgccatca ccactatcac ggacgatggt gcgcgccgct tgcgcactct gttccagcgc
     2281 cagcaggctg atttggtcag cgtaagcaaa tccggttttt tcaccgctga ttgcacgcac
     2341 gccaacgccc tgatcgatgt tgtaagaacc atctttaata atgcggtctt ctaaaaccca
     2401 ggattcgtga tagctcgact gaaaatagag atcgccataa tcaaggcgac gttcggccag
     2461 ttgaccgagg atcgcgaaca agtcctgatg tttcaggccg ttcgccgcta gcaattgttc
     2521 acttactagg ttaagactca tcgtttttgc tactcgttag ttactgcagt agaggatttt
     2581 ttacggctgc cggatgcggc gtgaacgccc tatccgacct acggttatgt tctggctcgt
     2641 tggcctttgg caacgattat catatgagat tggggcaatt acgcgccctc gtcaaatcat
     2701 tgcgcttttt ctttacgcgg ttggcgcaac acttcgttga tttgcggatc gtccagcgga
     2761 cccgaaatgt gatagcgcaa aatggagact ttgctccaca gcggccccag cactttactg
     2821 gctgcaaata ctgccgcgcc aacaatgggg ttaaccgcaa aagccgcagc cacgcccacc
     2881 gtcgcagaaa tctctggtgc gacaaccgct tccatattca ggtcgcgacg taccagattt
     2941 accgaccctt tcatggcgat atccgcctcc aggccatcca ccagcgtgtc gtcggtgtgc
     3001 ataacgccgt ctttaatcca cgcggtgctg cgaatggagt caaaatagaa cccttcgcca
     3061 aaagtgtctc tgaaatcaaa acgcagctta cgcatcaggg catcaacgct caataagcgc
     3121 agcaattgcc cggcatgtcc ggtattgatt tcggtaattt cgcctttacc cagttgagta
     3181 tgaatgatgc cattcaacgt cgcctcatct ggttgccagg gtgctttgcg ccagtgtaaa
     3241 tcgtaatcca cattaaatga cgactggcgt atgggcgtcg tgacaccaaa aaattctgcg
     3301 gcggcatcaa ttttctgccc gcgcagtttt cctttcagcg aggtacgttc attccccgga
     3361 ttattaaccc attcaccatc ggcagtaagc cgcgagaaac cagtatcaat cagtccattg
     3421 gtcagcgtta acgtatcgcc agaaatggtg agatcactgt caatacgacc gaatttttgc
     3481 ccccagaacc agcactctgt gcatcgtatt tgtgcgtccg gccagccacg gaagttaatg
     3541 cgctccgttg tcgggaaagg tgatgacggt gttgaatcac cacgagtttt cgccacgctc
     3601 gggttgtaat aaagatattt gatattcgcc agccacggcg cgttattacg catcgccagc
     3661 gtggcattga tttcacgccc ctgcgcctca accagggtgc catttgccgt cggttgcgaa
     3721 acaatactca ggttattcca ttgctgattt cccagtgaca acataggcgt acgtaacgtt
     3781 atgtgttgtg ggaaactcgc tgcaccaccg acactctccg cagcgccttt ctggaacagg
     3841 gccagccact cggcaccatt catcggcggc atattgagtt caacgccact ttgttccggc
     3901 aacggcggga gcgttttact gtctgccgcc caaatagcac gatcgagcgt cagcttttga
     3961 ccaagcaacc agcggctatt gaaatggtta tccgcaccag cctgtccggt taattcaaag
     4021 ctgctgagat tgccatcaac cttaacgttt accggcagtg gttcacccgc aggtttggct
     4081 aacggtgaag gtaagtgact gctcacatta ttcagatcgc cgttcagctc tacgttatag
     4141 gtcgcgccag catgataagg cagatcaatg cccactttac catcccacgc cacgctgcca
     4201 ctcaatgctt cgttcaccgc tgcaggcaga acgccggttt tcgccggttg ccagttaccg
     4261 ttgaggttta ccgcaacctg gtaggctttt gccccttctt tggtggaaaa atccacgttc
     4321 aacggctgat taaaccagct tgctgtcagt ggttcacttt gcagatcgcc attgataaag
     4381 ctgaatttac cgctcaaatt tttcagggtg ctgtcgagta gtttgataaa cagactgtta
     4441 ttacgcagcg tcacttcacc tttcgcggtt accagttcgc cgttcagcgg gatatcaaga
     4501 tgtaagcgag cattcacatc gccgtcgagc tggagttctt gcagggtcgc acccagagaa
     4561 tctttcagcg gtgtctcatc aaagtaaggg ccaacggctt tacccggacc tttaatgtca
     4621 gcgtcaatca gcagtttttc tttcgagtag tcagggatca ctgcggtgag attactcgcg
     4681 cgcacgccgc ccagattaac gtcatcggtt ttcatccata aaccgtcgtt aataaagtcc
     4741 agttcaatat caaggttagt taatgcaggc cagtccggct ggaaggcaaa cttcgcgtta
     4801 cgcagcggca ccagcacttc aaactgacct tcgttgtgtt tataggggaa gagttgcgga
     4861 ttgccaccat aaaccagcgt cgcgttatcc gcttcaccgc cctgaatcgc gccacttaag
     4921 taatcaacta ggtctttacc catcaagttt tccgggaaat agcgccaggc ttgtgaacca
     4981 tcatcggtac tgatgccagc cagaataccc agccagggtt catcgttagc aggttgcagg
     5041 taacgaaaac cgccgcgcgc atggacagct ttggctttaa cgtcaatatt acgcccatcc
     5101 aggtggaaac ctttgtcatt gttcagccag cttatagttg cctggccgtc ggcgatttcc
     5161 agtggcgcac ggaataccgt ttcgtaaggc atctttgcct gcttcatcga cgcggtaagc
     5221 aaaccatttt caacgctgcc ggaaagcgtc ccggagaagt gttccgcacc cggtaataat
     5281 ttccattgct tccaggccag atcgctccac gatgcctgaa aacgggtctt gtctgccgcc
     5341 tgaagcggga tatccagcgc cagagtgtta atcttgccgc tcggttgtgt ggagcgccaa
     5401 acatcaccca gtgcaggtga aagtttcgcg gccagcgggc gtatgccctc caggcctgcc
     5461 agctccagat tactggcgcg aatccggagt tcgtcactgc gtttattgtc tttgccgcca
     5521 acgtcctgtt ccggtatcca ggccagcgtc aatgctccgc tcggccaggg tttgccgtcc
     5581 atcgtgatcc gtgtatcggg aatagagaac tgccagcccg gattttcacg cgtaatatgc
     5641 gcggtcagat tatccaccga cagcgtatgc gtttgcttct cgcccaacca gctggcaccg
     5701 ccctgtttca gccagacgtc accgccggtt acatcgcctt tgtcgatcgt catccagcct
     5761 tcaagggaga actgtgccgt ttccagagca atattgtcct gcatccattt accgagccac
     5821 ggcttcaggt cgatgtcatc cgcctgtagc cagacgcgac cattgcttaa caacccctca
     5881 tcatcgcgca aatccatgcg cacctgcatc acgccgtgct gtccggtaag gctggagagg
     5941 cttaccaggc cttccgcacg gtgtcgacgt ggatcgttca gccaggtgag ttgtgggatc
     6001 gccagctcgg cgcgctgacc ggatggcgtc agaaaactga cttcactgtc gcggagatcg
     6061 aaatggtcaa attgacgaag aaacagatcg ctgatgtgac tggcttccag gctatcatta
     6121 ccaccaccgc tggtgatagg agtgttggtg cgaaagcgca gctgccagaa agtgaggtcg
     6181 cgaaactgcc agcgcatatg taacaagctc tgccagacat ccagcgccag agtaacgcgt
     6241 ttaaccgaaa attcgccgcc atcttttagt tctgcacgga tgtcgtgtgc ttcaagcgtc
     6301 gggccaaaat tctgccagct ggctgagagc tgactggctt ctaccggcat gccagtcgcg
     6361 gattctattt tgttgaggat ttccggacgc caggcgtcaa gatgcggtaa agcaatacgc
     6421 aggccgctaa ccagcagggc tgcgatcaca acgagcgcgg ctccagtaag cagtaaaatc
     6481 cccggcaatc gcctcacccg tcactccttg tctgctaaaa atgtgactca aaaacccttt
     6541 gccggatggc ggcccagcat ctgtttacat cattacgacg tcaaactgct cctggttata
     6601 gagcggttca atttgtactt taacctgttt gccaacgaaa atttccactt ccgccagcga
     6661 gtgtgactct tcgcctttca aggcttcagc tactgccgga gaagcataga ccaggaaacg
     6721 gtcggagtcg taagcatggt ggacacgaac aatctcgcgc atgatttcat agcataccgt
     6781 ttccacggtt ttcaccgttc cgcgaccgtg gcaggttggg cattcgttac acagtacgtg
     6841 ctcaatgctt tcgcgggtgc gtttacgcgt catctccacc agccccagcg ccgaaaaacc
     6901 attaacgctg gttttcaccc ggtctttgct caacgcctgc tccagcgagt gcagcacgcg
     6961 gcggcggtga tcttcattat tcatatcgat gaaatcaata atgataatcc cacccagatt
     7021 acgcaaccgt aactggcgag cgatagcctg cgtcgcttca atattggtat tgaaaatggt
     7081 gtcgtccaga ttgcgatgac cgacaaacgc tccggtattg atgtccacgg tggtcatcgc
     7141 ttcggtctgg tcgataatca gataaccacc ggatttcagt tctactttgc gttccagcgc
     7201 tcgctggatt tcgttttcga catcaaagag atcgaaaatc ggctggcgtc cggtgtaatg
     7261 ctccagcttg ctggtcatct cgggaatgta ctccgaggta aactccagca atgcttcgta
     7321 agtcaggcgt gagtcaacgc gaatgcggtc cagttcggcg tcggcgaaat cacgcagaac
     7381 acgctgcgcc agcgccagtt cgccgtacag ctgataacgg gtctgcgggc gttttttacg
     7441 ctccataact ttggtccaga cgcgtttcag ataagcggca tcggaggcca gttcagcctc
     7501 gccaacccct tccgctgcgg tacggatgat aaacccgccc tgctcgtcgc aatactctgc
     7561 gaccactttt ttcaggcgtt cacgttctga ttcgctttca atacgttggg aaaccccaac
     7621 gtgagaagcc cctggcataa acaccagata gcgagaaggc agcgtgatat cggtggtcag
     7681 gcgcgcacct ttagtgccaa gcggatcttt caccacctgc accatcagat cttgcccctg
     7741 acgaaccagt tccgagatgt cgcgcaccgt gaattgcttt tgttcttcac ccgccacaca
     7801 ttcggtgtgc ggcatgatat cggatgcatg aagaaacgcg gctttatcca gcccaatatc
     7861 tacaaaagcc gcctgcatac ccggaagtac acgacttaca cgacccttgt agatattgcc
     7921 tactattccg cgtcgcgcct cacgttcaat atgaatttcc tgcagaatac cgccatcaat
     7981 atacgccact cgcgtttccg aaggcgttac gtttactaac aattcagccg tcatgtttat
     8041 cccttttctc acgcagtgcg ttaaaattac ttaataattc atacgtttca accagcggta
     8101 agccgactac ggcgtgatag ctgccattta tcttcctgac aaaacagcca cccagcccct
     8161 gaataccgta tgcacctgct ttatctaacg gttcaccgct ggcgacatag cccgcgatgt
     8221 cttcgtctgt taacgttctg aaagtcacat cggtgaccac caggcaatcg agaatgtgct
     8281 ggctgtcggc caacaccact gccgtcatca cctgatgggt ctgacccgat aatttgcgca
     8341 acatctgcgc cgcatgctct gcgtcgcgcg gtttctccag cacttctcca ttcaggataa
     8401 cgatagtatc cgcacccagc accgggagat cctgcgccgt ttgcgcgaca cccgcctgtg
     8461 ctttcttgtg tgccagacgc acaacatact gctgcgcgct ctcctgcggc tgacgctgct
     8521 cctcaatgcc cgtaacaata cgttcaaagg tcacgccaag ttgcgcaagt aactcctgac
     8581 gacgcggaga accggaagct aaatacagag aagtcataga aacctttatt gcactgcaaa
     8641 ctgctgacgg actttacgca tcagcaagaa aatccacggc cagagcaccc cattgactac
     8701 actactccag aacacttccg gtctgaaaga gacgttaatc actaaaaact ctgcccagaa
     8761 aacaataata tccaccacca gcgaaagcaa catgacgact agcgcctgct gccataatgc
     8821 gaggttgcgg aaaagctggt atttcagcgc caccaggtaa gcaatgatgc tcatcgccaa
     8881 tacgcgtacg ccaagcgtcg agccgctgat cagatccagt atggcaccca tcacaaaacc
     8941 tgtgcccaca tttacgcgat ggggcaaggc caggatccaa tacaacaaga taagtaacac
     9001 ccagtttggc cggaaaacaa tcaggttatc cggccagggc atgatttgca gcaacagcgc
     9061 aatgaggaaa gagagccaga ttacccaacg tccctggcta cgatagctcg ccactattgc
     9121 cctcccggcg cacgcgcagg cggttgagca ccactttgcg gcggcgtagc cctttgtgga
     9181 gagcgattag cagcaggcgc agtagctgca tttcctgtcg tcggttgctg cggagtcggc
     9241 tgagcgatcc ccgttgtcgg ttcaggtaac tttggcccca tcgcgtctgg cgaaggcaat
     9301 acctgcggca tcatctgcat cagacgttca ttagcaacac gatgcacctc ttccggcgtc
     9361 atcgggttag cgccgttacg atccgcgccc cacagcagca gcagataacg caaacgttgc
     9421 agtcctgcag tcggacgcgc ctgaatcaca gtataagcgc gctgggtatc gagttttacg
     9481 gaagagacaa ccgcgaccgg atagccttcc gggaaacgac cgcccagacc ggaggtcacc
     9541 agcacatcac caacacgaat atccgtattc gctggcagat gctcaagctg caaatcatct
     9601 gtacaaccgt taccggctgc aattacgcgg atatcgttgc gcagcacctg gattggcagc
     9661 gcgtgggtcg catcacaaat cagcagcacg cgactggtca gtttagcgac ggccaccacc
     9721 tgaccaacaa cacctttgtc gctgatgacc ggctggcctt cataaacgcc attaacgcta
     9781 cctttatcga taacaacttg atcgctataa ggatcgttaa ccgtggagat aacctgagtc
     9841 accattttct gctcatcctg acgcagcggg gaacccagca gctcgcgcag acgcgcgttc
     9901 tcctgtttgt attgtccaag catcagcagt tcactgtttt tcagcaacag ttcctgacgt
     9961 aacgcccggt tttcaagttc tagttggtca cgcgaggcca gcgtctgcga tacgccatcc
    10021 agcaattcac gaggagcatt ggaaacaaag tagaaaggac tgacggcggt atccatataa
    10081 gtacggattt gactgaacgt ccccaggcgg ctgtcggcaa taataatgcc gagcgccacc
    10141 agcaccgcca gaataaggcg aatctgtagc gacgggccac ggctaaaaat tggcttcata
    10201 agttatgcgt attctcgtat cagactaggc agggtaaaca gacatttccc ctgcctgcat
    10261 ccgattactc ttcgctgaac aggtcgccgc cgtgcatgtc gatcatttcc agcgctttgc
    10321 cgccaccgcg cgccacacag gtcagcgggt cttcagcaac aacgactgga atgccggttt
    10381 cttccattaa caaacggtca aggttacgca gcagtgcgcc accaccggtg agcaccatgc
    10441 cgcgctcgga gatgtcggaa gccagttccg gcgggcactg ttccagtgca accattaccg
    10501 cgctcacaat accggtcagc ggttcctgca gtgcttcgag gatttcgttg gagttcaggg
    10561 taaaaccgcg tggaacacct tctgccaggt tacggccacg aacttcgatt tcacggactt
    10621 catcgcccgg gtaagccgaa ccgatttcgt gcttgatacg ttctgcggtg gcttcaccga
    10681 tcagagaacc gtaattacga cgcacatagt tgatgatagc ttcgtcgaaa cgatcaccac
    10741 caatgcgcac ggaagaggag taaaccacgc cgttcaagga gataacagca acttcagtgg
    10801 taccaccacc gatatcaacc accatagaac cggtcgcttc agaaaccggc aggccagcac
    10861 caattgcggc agccatcggt tcttcaatca ggaagacttc acgggcacca gcgccctgcg
    10921 cggattcacg aattgcgcgg cgttcaacct gggtcgcgcc aaccggtaca caaaccagaa
    10981 cgcgcgggct tggacgcata aagttgttgc tgtgcacttg tttgatgaag tgctggagca
    11041 ttttttcagt cacgaagaag tcggcgataa cgccgtcttt cattgggcga atggcagcaa
    11101 tattgcccgg cgtacggccc agcatctgct tcgcgtcatg acctactgca gctacgcttt
    11161 tcggtgaacc ggcacgatcc tgacgaatgg ccaccacgga aggctcattc aatacgatgc
    11221 cttgtccttt tacataaatg agggtattcg cagtacccag gtcaatggac aagtcattgg
    11281 aaaacatgcc acgaaatttt ttcaacatac taagggataa tcctgaaagc tggggcggaa
    11341 aagaaaatcc gcttacttta ccaaccacac gcagcagcga caaggcgcaa aaatcatctg
    11401 ctacggtgaa aattagtgca gttcgtttcc tttgttacaa atctctgcct gagtccagaa
    11461 aggcttaatg catcagcagc attcctcgcc tgtttgcaac cgcgtaaggt cattcatctg
    11521 catatgtgct gcaacaatct ggcgagcaga caagcacact cccatgagac gcagcgcgcg
    11581 ttattctacg tgaaaacgga ttaaacggca ggttaaaccg agtatctttg tgaatatttt
    11641 ttcacgttag tatcaagtgg ctgtgaggac gcgaaaaaat ccccttgccc gcctgtaaca
    11701 ccgcgctgaa tcagggtctg ccactcgctt cgcgaacgca caccggtggc gtaaacctgg
    11761 gtgctggtcc cggagcaggc ttccaccagg ctttgaacca gcagctggtt ctccgttcgc
    11821 ttctcaatgt ttctgaccag ccccggatgg agcttgagta actcaacatt aagttctttg
    11881 atccaactgg tgcttaccag cgtcaaacca gcctggttga cggctacccg tacccctaaa
    11941 gcattcacta aacgaataac aggttgtaaa cgactgatat gttgacctac atcggcctct
    12001 gcaagttcaa taattatgcg ttttcgttgt gatttttcac attgcattaa cgtatcgcgc
    12061 agccaacgct gaaaacgcgg gcgaatcagc gactcaacgg taacctgaat cgccagattt
    12121 tcctctggcc agtaacgcaa tagtggaata agacggctga tttgcagacg gtcatactct
    12181 tccgataagc caaactgcaa gaccatcggc atatactccg ccgagctaac ctcttcatta
    12241 ccatcgaaga tgcggcacat gagttcgcga tgatgaacct gaccttcgcg agtaaccgcc
    12301 ggtttttgat aaaggcgcgg gccgccgcga ctgagcattt gctcgataag cgtacgccag
    12361 cgaacattac cgcgtccttt ttcaggcaac gagtcatcgt aaatagccca gctattgccg
    12421 ccctgcaatc ccgcattacg cgtggcagac tctgcatgtt ccattacctg ctcggtatcc
    12481 tgaccactac gccaggcgca gataccaatg tggatcatat cgtcgcgatc gagcatttta
    12541 ttgttcggca aggtatcgac ggctttgatt aactgaccgg cgatgctctc tgcctctttt
    12601 aacgtccggt gcggtaacag cgcagcaaaa tcactgcggt ggtaacgcgc cagcagtgcg
    12661 ccagggtagc gcatcataaa tgtcgacagc agattcgtca gagtgaagaa ctgttcttca
    12721 acctggctgt gcccccaagt atcgctcaac atattgaaat ccggcagacg aatcatcatc
    12781 acgatcccgt gggtacctac tttctcctga tcttccagta acgttgctaa ctgattatcg
    12841 aaaaagagtc ggttattgag gccggttttc acgtcctggg cggcataaga gcggatcagc
    12901 gtatcaagac ggctgtgttg ttcgcgtgcg ttctgaattt cacgaagcag cgtatccagc
    12961 gcactgctgg ttctgggcgg ccattcatag atggttccca acacattaga gccacgctca
    13021 ccgtttaaga tacgagtagc ccgggtttcc agcaattctt gcccggcaag ttgccgttgt
    13081 aaccagcgta ccgccaagaa gagcataaca atgataaagc taatcgcccc cgtgagcggc
    13141 gcggtggtca tcaacgaatg gaaatagttg cccatcggat cctgataaac cagacgcagc
    13201 gacatccccg gatactttat caacggaacg ctcagttcac gaaacagatc gctggagcca
    13261 accggacgat aactaccatt tctggccagg gtataaacct gtttatcacc atggagcagg
    13321 tctacacgaa cgatatctgc cgacatcatt aattcggtaa tttgtggcct taatgtgctg
    13381 aagtcattcg acacaaggtg ggtatcaatc gccgtcgcca ccgcctgaac gcgatgacta
    13441 aacttatact gaattgcgtt gtagaaactt agcgaacagc ccagcaaagt cacaaaaatt
    13501 gttaacccgg tgagcagcgt aacaaaggcc gaaaatttcg tcgttaatct catccttgtg
    13561 ttaactccga tagtgaggaa gcgggcatac tagcaaatca gatttatctc gcaatttatt
    13621 gcgcttcatc ggctttgctt ttccattagc gagtataatc ttcagaaatt attttccaat
    13681 ccatcatgca catgaggacc acttatgcag gcgttacttt tagaacagca ggacggcaaa
    13741 actctcgcat cagtacagac tctggacgaa agtcgcctgc cggagggcga tgtcatggtc
    13801 gatgttcact ggtcgagcct gaactataaa gatgcgctgg cgattaccgg taagggaaaa
    13861 atcatccgta attttccgat gattcctggg atcgattttg ccggaactgt acgcaccagc
    13921 gaagatccgc gttttcatgc cggtcaggag gtgttactca ctggctgggg cgttggtgaa
    13981 aaccactggg gtgggctggc ggagcaggcg cgagtgaaag gtgactggct ggttgccatg
    14041 ccgcaagggc tggacgcgcg taaagcaata attatcggta ctgccggttt taccgccatg
    14101 ctgtgtgtga tggcgctgga agatgccggt gttcgcccgc aggacgggga gattgtcgtg
    14161 acgggtgcca gtggtggcgt cggcagtacc gccgtggcgc tgctgcataa gttgggttat
    14221 caggtcgttg ccgtttccgg tcgcgaaagt acccatgaat atctgaaaag tttaggtgcc
    14281 agccgtattc tccctcgtga tgagtttgcc gaatcccgtc ctctggaaaa acaagtctgg
    14341 gctggggcaa ttgacactgt tggcgacaaa gtgctggcaa aagtgctggc gcaaatgaat
    14401 tacggcggct gcgtggcggc ctgtggtctg gcgggtggtt ttactctgcc aaccacggtc
    14461 atgccattta ttctgcgtaa tgtccgtttg caaggggtgg attcagtaat gacgccacca
    14521 gaacgccgcg cacaagcctg gcagcgactg gtcgccgatt taccggaatc attctatacc
    14581 caggcggcaa aagagatatc tctgtcagag gcaccgaact ttgccgaggc catcattaat
    14641 aaccagatcc agggtcgcac gctggtgaag gttaactaac catttagcaa ggaataataa
    14701 gagagggaac tcattttgac atcattattc aatcggttaa cgggaaaagc ggttagccgg
    14761 acagctttcg tcgaacacct tggtcaggaa gttgtacaac atcatccaaa ctggaaagtc
    14821 atgatttcga ctgaccacaa attgatgcgc attgatactc ctctaaacag ccattattga
    14881 tacgcctccg tcgcctgtta ggtttatggt gctttgcctg ggcgacacta cgcttaacct
    14941 gttactgatt tctggtacca tggggtgaat aatctgattt tatttgactg caaattaacc
    15001 actcgaacct atttaatgct gagaattgtc agtcggttga ttttgcgtgc tttagcattc
    15061 acatctatcc agacgatgca gtgaaaattg ggtaatcccc agcaaccgct gcgtaatgtc
    15121 gtctatcttg tcgcgatcct ggcatcccta cattatttgt ggtctgtgaa gattatctca
    15181 ttgcagcccc tcatcttcgc agggctggct ttacagcttt tcaccttacg ttataagaag
    15241 ttccgtcgat gatggcgcta atttcgtgaa ttgtgcggct tgttgcaaat tacacggtgt
    15301 tgaaggttat ttacatgtta gctgttgatt atcttccctg ataagaccag tatttagctg
    15361 ccaattgcta cgaaatcgtt ataatgtgcg acctcgtcct ccctgacgca gtttttgcgc
    15421 tgcggaaaag gtgacattgg cgcaacgaag gtatattttg ttttttgccg gaggatagca
    15481 gcagatcgct gcacaatgtc cgtcaagtct aacattgata ctctggggca aaatagaccg
    15541 gcgtcccggc ctgctggaat ttatcgctat gcatacagct gtcggggcat acgctttaca
    15601 gacggcggtg aaacgcctgt cacaatcaca ctaaacaaag agtacggaac ccactcatgg
    15661 atattcgtaa gattaaaaaa ctgatcgagc tggttgaaga atcaggcatc tccgaactgg
    15721 aaatttctga aggcgaagag tcagtacgca ttagccgtgc agctcctgcc gcaagtttcc
    15781 ctgtgatgca acaagcttac gctgcaccaa tgatgcagca gccagctcaa tctaacgcag
    15841 ccgctccggc gaccgttcct tccatggaag cgccagcagc agcggaaatc agtggtcaca
    15901 tcgtacgctc cccgatggtt ggtactttct accgcacccc aagcccggac gcaaaagcgt
    15961 tcatcgaagt gggtcagaaa gtcaacgtgg gcgataccct gtgcatcgtt gaagccatga
    16021 aaatgatgaa ccagatcgaa gcggacaaat ccggtaccgt gaaagcaatt ctggtcgaaa
    16081 gtggacaacc ggtagaattt gacgagccgc tggtcgtcat cgagtaacga ggcgaacatg
    16141 ctggataaaa ttgttattgc caaccgcggc gagattgcat tgcgtattct tcgtgcctgt
    16201 aaagaactgg gcatcaagac tgtcgctgtg cactccagcg cggatcgcga tctaaaacac
    16261 gtattactgg cagatgaaac ggtctgtatt ggccctgctc cgtcagtaaa aagttatctg
    16321 aacatcccgg caatcatcag cgccgctgaa atcaccggcg cagtagcaat ccatccgggt
    16381 tacggcttcc tctccgagaa cgccaacttt gccgagcagg ttgaacgctc cggctttatc
    16441 ttcattggcc cgaaagcaga aaccattcgc ctgatgggcg acaaagtatc cgcaatcgcg
    16501 gcgatgaaaa aagcgggcgt cccttgcgta ccgggttctg acggcccgct gggcgacgat
    16561 atggataaaa accgtgccat tgctaaacgc attggttatc cggtgattat caaagcctcc
    16621 ggcggcggtg gtggtcgcgg tatgcgcgta gtgcgcggcg acgctgaact ggcacaatcc
    16681 atctccatga cccgtgcgga agcgaaagct gctttcagca acgatatggt ttacatggag
    16741 aaatacctgg aaaatcctcg ccacgtcgag attcaggtac tggctgacgg tcagggcaac
    16801 gctatctatc tggcggaacg tgactgctcc atgcagcgcc gccaccagaa agtggtcgaa
    16861 gaagcaccag caccgggcat taccccggaa ctacgtcgct acatcggcga acgttgcgct
    16921 aaagcgtgtg ttgatatcgg ctatcgcggt gcaggtactt tcgagttcct gttcgaaaac
    16981 ggcgagttct atttcatcga aatgaacacc cgtattcagg tagaacaccc ggttacagaa
    17041 atgatcaccg gcgttgacct gatcaaagaa cagctgcgta tcgctgccgg tcaaccgctg
    17101 tcgatcaagc aagaagaagt tcacgttcgc ggccatgcgg tggaatgtcg tatcaacgcc
    17161 gaagatccga acaccttcct gccaagccca ggcaaaatca cccgtttcca cgcacctggc
    17221 ggttttggag tgcgttggga gtctcatatc tacgcgggct acaccgtacc gccgtactat
    17281 gactcaatga tcggtaagct gatttgctac ggtgaaaacc gtgacgtggc gattgctcgc
    17341 atgaagaatg cgctgcagga gctgatcatc gacggtatca aaaccaacgt tgatctgcag
    17401 atccgcatca tgaatgacga gaacttccag catggtggca ctaacatcca ctatctggag
    17461 aaaaaactcg gtcttcagga aaaataattt gtgacgcgcg gtgttttcat cgcgcttagc
    17521 aaagaacgta atcaacgccg ggcctgttaa cgctgcccgg cgtttttatt gccgttagct
    17581 aacggtagcc tgcaatttcc gctttcaacg cgctcatttc agcatcgcca tcaacaattc
    17641 ccagctcaaa gcagtatttc tttgtttccc ctggctcaat aaagggcaga cgcccctgcg
    17701 ctcgttcttt atcacgaccg tcaggaaaac tattactgac ctccagtccc ataacatact
    17761 cacccgcgcc taaatttttc cactggataa agttgggtag ctgcatggtg tcgaatttct
    17821 cgtaaattcc ccaccccagt tcggcgttca ccagagcaac tttactcatc ccctgactgt
    17881 cgctattcaa cgtgagatag taaacctgct cgtcaaaacc gggctgtggg gtacaaatct
    17941 ccagatgact ggttaaccct tccgccgcat gcggagttcg tggcgttacg cttttagccg
    18001 acagcaagat ttctggcctg tggcgataat aaaggccagc cgtaattaaa gtgataaaga
    18061 atttgcagcg gctggcgggt gaaaccatga ttagtcacct tgtcttcaat gcgcagcaca
    18121 ttttcgccat aacggcagcg aatggtcctt tccagcgtca gattttcgcc gaacagtcgc
    18181 gtttcccgta ctttgccatt gatgacaaat tcaatgccgt cgtcggtccg ttctgtgcga
    18241 taccccacct cttctgctgg agtcgcagcc aaacgcccgt gtaaccccag cgtttctcct
    18301 tcatcctcac aaggcgtgcc aatgtagcta agtccgcagg tagtcagtaa ccccgcgtaa
    18361 aagctacgta aaaatccgtt gccctgagac tcaaaaaaag cgggggcgac aatgccgttg
    18421 ggggtgataa aagaaagcgc cgtgtccttg tagctggccc atgcaatatc cagcgcgcga
    18481 tcttttacca ccgtaaatgc cagtcccgca ccagtacgga catcaaacgc ttctgtaccc
    18541 tttgctctgc cactacgtaa ctcatagtgt ttaattccgg caacctgcga gagatcgcca
    18601 atccacgacc aaatttcgtt tttcattgtg ctgcttcccg aatggacatg tcatggcgct
    18661 ggatgaacgc ttcgatttcc gcaaggcgag acgttccctc cattgggcca cgtctggtta
    18721 ccgccagcgc accgcaggca ttggcatatt gcagcgctcg atggacgtca aaccctagct
    18781 ggcggcaggc aatccacgcg ccaccaaagc agtctcccgc accggtcggg tcaacttctt
    18841 ccaccggata gctctcaaca tgaaactgct cattcgcgga gtagtaactg gcaccttgat
    18901 tgccgcgctt cacaatcacc tctttaacac cttcttcaag aaaacctgcg attgcacgct
    18961 ccggcgtcga atgtggcgac aagagcaaca cttcgccctc gctgggcata tagatatccg
    19021 ttaactcaag aacaaagtgg agggcgtcac gcatttctgg aatatcgagc atctctttac
    19081 gaatattagg gtcgaaagaa ataacaccac catttgcttt aacgatagtt accgcttttt
    19141 ttactgcgtc taccatatga aacgaaaaca gggaagagcc cataatatga aaatgggtac
    19201 agtccttcag aatattttca tcaacatgct gcgccgataa tttaccgcaa gcggcattct
    19261 taatattaaa aataaaatcg cgatcgccag agttatgata agtaacaaag gcactcccgg
    19321 tagcttccag aggtaagact gaaatccccc tgatatccac accgtcagca gccagacgat
    19381 gtatattaat atcaccaaag ccatcattcc ccacacagct aatgatgccg cacggaacgc
    19441 caagacgtgt cacctggtcg ataaaaatag cgggcgcacc actgggataa gggccgttcc
    19501 agatacctgg ttgatcaaat ggctggccga ttttactcgc cataatttca accagaattt
    19561 ctccaattgt gaagacctta ctcatatgat taattccttg aaatgattag ctgcgttttc
    19621 ttttatttct tacgtcaaaa taaacggcta caagaatgac taatccttta accaccatct
    19681 ggtaaaaata gggaacattc attaagttca tgccattatt aataacgccg atagttaatg
    19741 cgccgatcac cgtccccacc acggttccat agccgccgtt caggctggtg cctcccagta
    19801 cagccgcagc aatagcatcc agttcgtagc tcaacgcgga attcggttac ccggaataga
    19861 ggcgtgaggc gaggatcatc ccgctgatac ctgccagcac ggctgtaatc ataaagactt
    19921 taattttcaa gcgggtgaca tttatccctg aataaattgc cgcttcttta ttaccaccag
    19981 tgatataaat ttttcgtcca aaggttgttt tagtaagaat gaagtggtta atcagcaata
    20041 agatgattaa tatccagatt ggaataggta tatagagaaa ttctccattc cccagtgcca
    20101 gaaaggcatc atctttaatc atgatgggca taccatcggt cactatataa gcgataccac
    20161 ggaagatccc catagtagca acggtgacaa taaacgacgg gatccccgct atagccgtca
    20221 agccaccatt tatcatcccc agagccagtg ctgcaataat ggataatggg accaataaaa
    20281 taaatggaat tccccatgaa gccccaagcg cagcaaatgt gccaatcaat gcaatggttg
    20341 aaccgaccga taaatcaata tcgccaagca aaagtacata agtcattcca taggcggtaa
    20401 ttgcaattat cgaaacctga agaataatat tagtaatgtt gttggcggta aaaaaactgt
    20461 cattaataaa actgaacaga atgattaaca ccaccattcc actgaggata ccgccatatt
    20521 gcctgaagac ttttttaaaa ttatttgtaa tgggaatttc cgaaggtttc ggactggcaa
    20581 gagttttctc tgtcatttaa cgtactcctg ttgcagcggt cattacctgt gtctgcgtta
    20641 aattttcaca tgaataaata tcggcaatcg ttttattacg cattaccacc agacgatcgc
    20701 taagtgccat gacttctggc agttcagagg acaccagaat aattgctgtc cctgccgcag
    20761 ccagttgacg tatcacgtta tagatttcaa attttgcgcc gacatccact ccgcgcgttg
    20821 gctcatcgag caataaaata cgcggagttt tctccagcca cttcgataac acgattttct
    20881 gttgattacc gccgctcagc gctcccactg cctgatgtac agaactcact ttagtgttca
    20941 ttctggcaat tgattgcagc gtcttcgcct cttcttgtcg gtgattgatg agtcccatcc
    21001 gcgaagcgtt atattgaaac gccacatggt gcgtgttcat attgacgtcg tgcatcagca
    21061 ctaacccttc ttccttgcgg ttttcggtca caaaagcaat gccatgatca atagcctgtt
    21121 taggattagt gatcctgact tccttaccat caatcaacac agtcccctga tacgaaacga
    21181 ggccaaagag cgccttcatc acatcggaac gcccggcccc caccaggcca aaaaatccga
    21241 ggacctcccc cggacgcaca gcgaaactga tctctttaaa aagcgaagga tgggaaagat
    21301 tttttacttc cagttttgca ggaacatttt catccggtat ttccccaaga cgtgctggcc
    21361 aaatattttt catttcccga ccaaccattt gggcaatcaa atgctggata tcactgtttt
    21421 tcttttcatg cgtggcgata tactcaccgt cgcggatcac cgaaatatca tctgaaatcc
    21481 gcataatttc atccatgcga tgagagatat aaataaccgc acagcctcgc tgctttaatt
    21541 tttcaataat gttaaacaga atctccgctt cattatcact tagcgaagat gtcggttcat
    21601 ccataatcac gacatccgct gcatagctta aggctttagc aatttctacc agttgcctat
    21661 gtgccatcga catattgccg accatttcta gtgggtctag tggtatgccc cgttccgcga
    21721 ggagattctg gcaatctgcg agcatttttt tatcgttaat aatgccaaaa cgtcgcggtt
    21781 cattattggc ataaatattt tccgccacgg tcatattatt actcaggctc aattcctgaa
    21841 aaataatcgc aattcctaac tggcgtgaat gctgcggatt attaatggtg atagtttcac
    21901 cacgtagtgt tatcgttccc tgggttggcg gataaacacc ggccagtatt ttcattaatg
    21961 tcgatttacc ggcaccattc tcgcctaata aagtatgcac ccgcccttta cgtaaagaga
    22021 gggtaatatc ttttaatgct tgaacatgac caaatgtctt cgcaatatgc gaaacatcaa
    22081 gaataacatc cgtcataatg acctcggtgt cacaacaaaa cgccaataac gttttgttgt
    22141 gaggtgaaga tgtttactat ttacctgtat aaacgccggg ttgtaccggg atcatttttt
    22201 ctaccggttt accattgagg tgatcaacag cggcatcaat ggctttagca cccatttgat
    22261 cgggatattg ccgaatcacg gccgcaaacg ttttttcact gtcaacggca tcacgcgctt
    22321 ctttcatgcc atcaaaacca atgattttga tgttgtcgtt atgcgcagat tttgctgcga
    22381 taaccgctgc cagcgccgca tcatcaccaa agccaaacag accattcagg ttggcatttg
    22441 cctgcataat gttttgcgca gtcgttaacg cttcagcccg cgtaatacta ggctgttcag
    22501 caacaatttt aatatccggt gtatcgctta accctttctt aaaaccatca acgcgcgcaa
    22561 ccaccgattg tagtgccgga taagaaataa tggctacagt acctttacca ttcaaaagtt
    22621 tgcccattaa cttgcctgct tcaacgcctc ccgcatagtt atcggtcgca acatgcgata
    22681 cgaccggaac gccttccgca gcgacgtcga cggtgataac gggaataccg gccttatcgg
    22741 ctttaattat cgccgcctgg acgcctttcg aatcgaccgg agagataat
//
LOCUS       DAJTOR010000053        22655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTOR010000053 DAJTOR010000000
VERSION     DAJTOR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.053"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..257)
                     /locus_tag="OO249_001825"
     CDS             complement(<1..257)
                     /locus_tag="OO249_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5636151.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDC"
     gene            complement(248..718)
                     /locus_tag="OO249_001826"
     CDS             complement(248..718)
                     /locus_tag="OO249_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5636152.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(903..1697)
                     /gene="thyA"
                     /locus_tag="OO249_001827"
     CDS             complement(903..1697)
                     /gene="thyA"
                     /locus_tag="OO249_001827"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5636153.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYAPHPGIKAPVAI"
     gene            complement(1704..2579)
                     /gene="lgt"
                     /locus_tag="OO249_001828"
     CDS             complement(1704..2579)
                     /gene="lgt"
                     /locus_tag="OO249_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5636154.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(2730..4976)
                     /gene="ptsP"
                     /locus_tag="OO249_001829"
     CDS             complement(2730..4976)
                     /gene="ptsP"
                     /locus_tag="OO249_001829"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5636155.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGLPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPILLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVVAFMERRGM
                     GGLIRGGL"
     gene            complement(4989..5519)
                     /gene="rppH"
                     /locus_tag="OO249_001830"
     CDS             complement(4989..5519)
                     /gene="rppH"
                     /locus_tag="OO249_001830"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5636156.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            6204..6893
                     /gene="mutH"
                     /locus_tag="OO249_001831"
     CDS             6204..6893
                     /gene="mutH"
                     /locus_tag="OO249_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5636157.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            6962..7675
                     /locus_tag="OO249_001832"
     CDS             6962..7675
                     /locus_tag="OO249_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5636158.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            7813..8031
                     /gene="ygdR"
                     /locus_tag="OO249_001833"
     CDS             7813..8031
                     /gene="ygdR"
                     /locus_tag="OO249_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5636159.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            8139..9179
                     /locus_tag="OO249_001834"
     CDS             8139..9179
                     /locus_tag="OO249_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5636160.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLNLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGGMRYLHLADKHDLPSIVTIQTPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKTAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(9211..10404)
                     /gene="lplT"
                     /locus_tag="OO249_001835"
     CDS             complement(9211..10404)
                     /gene="lplT"
                     /locus_tag="OO249_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5636161.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(10397..12556)
                     /gene="aas"
                     /locus_tag="OO249_001836"
     CDS             complement(10397..12556)
                     /gene="aas"
                     /locus_tag="OO249_001836"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5636162.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDASRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTLDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            12945..13976
                     /gene="galR"
                     /locus_tag="OO249_001837"
     CDS             12945..13976
                     /gene="galR"
                     /locus_tag="OO249_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5636163.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(13983..15245)
                     /gene="lysA"
                     /locus_tag="OO249_001838"
     CDS             complement(13983..15245)
                     /gene="lysA"
                     /locus_tag="OO249_001838"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5636164.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            15367..16302
                     /gene="lysR"
                     /locus_tag="OO249_001839"
     CDS             15367..16302
                     /gene="lysR"
                     /locus_tag="OO249_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5636165.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(16289..16981)
                     /gene="ygeA"
                     /locus_tag="OO249_001840"
     CDS             complement(16289..16981)
                     /gene="ygeA"
                     /locus_tag="OO249_001840"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5636166.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(17110..18525)
                     /gene="araE"
                     /locus_tag="OO249_001841"
                     /pseudo
     CDS             complement(17110..18525)
                     /gene="araE"
                     /locus_tag="OO249_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(18840..19601)
                     /gene="kduD"
                     /locus_tag="OO249_001842"
     CDS             complement(18840..19601)
                     /gene="kduD"
                     /locus_tag="OO249_001842"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5636167.1"
                     /translation="MILSAFSLEGKVAVVTGCDTWLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(19631..20467)
                     /gene="kduI"
                     /locus_tag="OO249_001843"
     CDS             complement(19631..20467)
                     /gene="kduI"
                     /locus_tag="OO249_001843"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5636168.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(20754..21935)
                     /locus_tag="OO249_001844"
     CDS             complement(20754..21935)
                     /locus_tag="OO249_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5636169.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            22190..22588
                     /locus_tag="OO249_001845"
                     /pseudo
     CDS             22190..22588
                     /locus_tag="OO249_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT         5809 a         6062 c         5456 g         5328 t
ORIGIN      
        1 atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagatgcc atgacaataa
       61 cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagc
      121 gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc
      181 cccagcaaca atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct
      241 tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt cttccccacg
      301 gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg
      361 cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg
      421 gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcagcgg
      481 aactcacaag gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat
      541 gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg gcggtttgcc
      601 atagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg cttagcatga
      661 ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc
      721 cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc
      781 ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggtg tctcgaagaa
      841 tttaatggag ggtaaaaaaa accgacgcac actagcgtcg gctctggcag gatgtttcgt
      901 aattagatag ccaccggcgc tttaatgccc ggatgcggag cgtagccttc aatctcaaaa
      961 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
     1021 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct gtacaggtgc
     1081 gtgtcgcccc ccgtccagac aaaatcgccc acttccagat cacactgctg cgccatcata
     1141 tgcaccagta acgcgtagct ggcaatgtta aacggcaggc cgaggaaaac gtcacaggag
     1201 cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa gaacgcatgg
     1261 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg
     1321 cgcgaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc aatatgacga
     1381 ccatccggcg ttggccaggc gcgccactgt ttaccataca ctggcccgag gtcgccgttt
     1441 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagtg
     1501 tcgccctgca gaaaccacag cagttcatgg atgatggaac gcaggtggca acgtttagtt
     1561 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
     1621 gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt
     1681 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc
     1741 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg tcccatgctg
     1801 atgtactgca cccaggcacc agtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
     1861 atgcgaaacg cgccgtaacc aatcaggaac aaacctgaga cagctcccat tgggcgcggt
     1921 ttacgaatat acaggttgag gataataaac agcactacac cttccagcaa cagctcgtaa
     1981 agctgtgatg ggtggcgcgg cagcacaccg taagtatcga aaatggattg ccactgcggg
     2041 ttggtttgca gcagcaaaat atcttctgta cgggagccag ggaacagcat ggcaaacggg
     2101 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
     2161 ccaagaccaa acggaatgag tggtgcgata aaatcagaga cctggaagaa ggaacgttta
     2221 gtacggcggg cgaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaggac
     2281 atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg cgggaagttg
     2341 tagaacagaa cataaccaat acgtccaccg aggaagacgc cgaggaagcc cgcatagagt
     2401 aagttttcaa cttcattttt ggtccagcca ctgcccggac gattcgcccg acgtgttgcc
     2461 agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg aagcgccacg
     2521 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
     2581 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
     2641 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
     2701 accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg
     2761 ccgcgacgct ccataaaggc tacaacctga tgacgaactt cggtcgccag ttgcgcttcc
     2821 agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa tgcgccgcag caggtatttt
     2881 acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt
     2941 gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat accatgtatt
     3001 tccgcttccc gggcgatcat tgccagagct cgtaacattg caggatgaag gctgtcataa
     3061 atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg
     3121 gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa taccattgac
     3181 ggtacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
     3241 acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt caacttcatc gaggctggtg
     3301 accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc
     3361 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
     3421 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
     3481 gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc
     3541 tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga
     3601 ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa accagcattg
     3661 agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc
     3721 gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg cagcagtatc
     3781 ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc
     3841 agcaccgaag gctgaatatc cgcgcccatc acggtaggga gccccagcgc acgtaccatg
     3901 atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg
     3961 ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
     4021 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca
     4081 cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
     4141 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
     4201 aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc
     4261 gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc
     4321 gcttcttcca gcgccccggt caatcgttcg cgttccagag ccggatccag cgttgatgcc
     4381 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
     4441 ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa caaggcagtc
     4501 aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
     4561 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
     4621 cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg
     4681 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
     4741 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
     4801 ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
     4861 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
     4921 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
     4981 ggcgtgactt aacctctttt acgtcgataa gcagatgtgt tttgtggttt tggcgtattt
     5041 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
     5101 ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac
     5161 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag ctgcaagaga
     5221 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
     5281 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
     5341 aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg
     5401 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
     5461 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
     5521 ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca ggaagacggt
     5581 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
     5641 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
     5701 gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat aagcccgata
     5761 taggcatttt aaaatattca ttataagtca atgcattaca tgtaatttga gtaatgaaaa
     5821 agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat
     5881 gccgtattta tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt
     5941 ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga ggtttttcac agttatccca
     6001 gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa
     6061 cctattgcag tgctgcgcaa ctcgattatc ggcagcctaa aaagcaatat aaatcagaga
     6121 attgaacaac acatgtggaa aaagttacac tgcgaatatt cggtacataa ttgctgtttg
     6181 ttttttaacc aaggtatcat gacatgtccc aacctcgccc actgctctct cctcccgaaa
     6241 ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg
     6301 cggcacttgc cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg
     6361 tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg
     6421 ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa
     6481 cattcgtttg tgttgccccg ttaacgggta atagcggggt gacctgggaa accagccacg
     6541 tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggcgagcgc agcatcccgc
     6601 tggcgcagcg tcgcgtagga tccccgttgc tgtggacccc gaatgaagag gaagaccgac
     6661 agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga
     6721 tcaccgctcg tcacggggaa tatttacaga taagaccgaa agcagcgaat gcgaaagcgc
     6781 ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt
     6841 tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc
     6901 tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta
     6961 gatgttattt gcatggataa ccgatcctaa cgcctggctt gcgctcggta cgctgacgct
     7021 gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct
     7081 tcccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg
     7141 tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg acaaatccgc tttttacaat
     7201 attcagtcag gaaatttctg cccgtgattt gattctgctt ctgggtggct tgttccttat
     7261 ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac
     7321 acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatctttag
     7381 cctcgactcg gtgattacgg ctgtgggtct gtcagatcac ctgtttatta tgatggcagc
     7441 cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga
     7501 acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtgg gctttaccct
     7561 gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt
     7621 ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa aagaatccgc tctgatactc
     7681 cgtacgctct cctgcgggag ggcgtactca ctccttttac cctcacagtt taagattttc
     7741 ctgctttcaa gatatataac gtcgttttat aaacagacta ttatcatagg taggccaaat
     7801 gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct
     7861 gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc
     7921 gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt
     7981 aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg
     8041 tcagcatccg gctggcctta agatttttct ctttcccttt tcccttccct ctgccatttt
     8101 tatattcctt atgtcgtgat tataaaaagg aaacggctat gcaatatcac cgtatacccc
     8161 acagttcgct ggaagtcagc acgctggggc ttggcacgat gacgtttggt gaacagaaca
     8221 gcgaagccga cgcccacgca caactcgact atgccgtcgc tcagggcatt aaccttatcg
     8281 acgttgccga aatgtaccca gtacctccgc gccccgaaac gcaagggtta accgaaacct
     8341 acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa gttaattatc gcctccaaag
     8401 tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg ctggatcgga
     8461 agaatatccg cgaagcgctg catgacagcc tcaagcgcct acagactgat tacctcaatc
     8521 tttatcaggt gcactggccg cagcgcccga ccaactgctt cggcaaactc ggttatagct
     8581 ggacggattc tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg gcagagtacc
     8641 aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga aactgcattt ggcggaatgc
     8701 gctacctgca tctggcggac aaacacgatc tgccgagtat tgtcaccatt cagacccctt
     8761 acagtctgtt aaaccgcagt tttgaagtag gtctggcaga agtcagccag tatgaagggg
     8821 tcgaactgct ggcctattca tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg
     8881 gtgcaaaaac cgctggcgca cgtaatacgc tctttagtcg gttcacccgc tatagcggtg
     8941 agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc cagacgtcat ggcctggacc
     9001 ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg
     9061 gcgcaaccac gatggatcag ctgaaaacta acatcgaaag tttgcatctg gagttaagcg
     9121 aagacgtatt agctgaaatt gaagcggtgc atcaggttta tacttatccg gcaccataag
     9181 attaacgccg gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga tccacagcgc
     9241 cgttattgcc agcgcaaaca gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc
     9301 taccattacc gccagcgagt aaatgcccag catcaacaac atggcgctgt tttcgccaag
     9361 gttttgtact gcaatcgcat tccccgcccc gacgcttttt ttaccccgct cctgtagcaa
     9421 cgcattgagc ggaacgacaa aaaagccccc catcacgcca atcagcatca acaaggcata
     9481 ggctggcagc agctcgtgtt gcagggaaaa aatcagtacc accacgccaa tcaaaatccc
     9541 ggctggcata cagcgtgaca cggtttccag cgtaactaac ttcgctgccg cacctgcgcc
     9601 aaccacgata ccaatcgcta ccatcgcgtt gagataggtg ggcgtagcgt tatcggtaat
     9661 gcccagcgcc accggtaccc acagcaccaa caggaaacgc agcgtgacac ccgctcccca
     9721 gaataaactg gtgcccacca gcgaaaaacg cgtttcacca ttgcgccata gcgaggtgca
     9781 ggcattcagg aaactgcggg tcatgttgat gagattccaa gactgccccg gacgcgccgc
     9841 cgccagtttg ggaatgtaga tattggcaac gaccgcacca ccgtaggcca gtgcgcatgc
     9901 ggccagggcg acgaggacat gccagtcagc cagcacacca ccggctacgg aaccgagcaa
     9961 aatcgccgct atggtagaag cttccattaa accgttagct ttcactaact tactacccgt
    10021 ggttaattcg ccgagaatac cgtatttcgc cggtgaatag gctgcagcac caacccccac
    10081 cagcgtatag ccgagaaacg gattgatacc aaagcagata ctggctgcgc ccagcagctt
    10141 caggccgttg gcaaacatca tcacccggcc tttggcgaag ctatccgcca cctgcccgac
    10201 aaacggcgca aaaagaatgt aagcacctac aaacaccatt tgcaggatgg gctggctcca
    10261 ctccggatag aactgcgctt tcagtaacgc cagagtggca aacagtaggg cattatcgcc
    10321 aaacgcagag agaaactgcg ccacgataac cgctttcatc cccttcgacc acaacgaagt
    10381 gttagtgtgc actgactcac tcatcgtgtt gttccgcttc gtctacccag cttttcaacg
    10441 tgacaaagtc aggtttgccg ctgccaagta atggcatctg tttcagatag cgaatatcgc
    10501 gcggtacagc aagctccggc acgccgtgct cgcgggcata ctgttgcaac ttatcgagcg
    10561 tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc tttgctggca tcgctcttaa
    10621 tcgcagtggc atggacttta tctggcgaaa caccaagtgc cagttgttcc accatttcca
    10681 gcgacaccat ttcgcctgca attttggcaa agcgttttgc gcggccctga atctgcacaa
    10741 agccctgctc gtcaaaacgc acaatatcgc cagtgtcata ccagccgcgc tccatttcgc
    10801 cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg cttctccacc cgcagatagc
    10861 cgttcattat gttcggccct ttcagttgca ggcgtccgcc ctcttcgata ccaggaaccg
    10921 acaacaggcg cgcatccatt cctggtagaa tacgccctac cgtaccgggt ttcgccgcca
    10981 tcggtacgtt gatagaaacg acaggcgcgc actcggttac accatagcct tcaaggatgc
    11041 gcaggccaaa tttatcctgc caaagctgtt tggtactttc ttgtagtttt tctgcgcctg
    11101 ccaccacata gcgtagacga tagaagtcat acgggttggc gaaacgcgcg tagtgaccga
    11161 ggaaagtcga ggtaccgaac aacacggtgc aactgcggtc atacaccaac tccggcacaa
    11221 tgcggtaatg cagcgggctt ggataaagga acacttctgc acctgtaagc agtggcgtaa
    11281 acaggcctac cgtcagccca aaggagtgaa acagcggtaa tgccgacata aagcgatcgt
    11341 tggtggtgaa gtcggcaatc gttttaatct gctcgacgtt cgccagaatg cttttatggc
    11401 tatggacgac gccttttgga tggccttcag aaccggaggt aaagaggatc agcgcctctt
    11461 cttccggctg ctgttttacc tgcgccaggc gcggcatcag caaatgagcg aagatccata
    11521 ctttgtcggc agtggtgaca tctgctttta aatcttccag atagacccag cgcacctgag
    11581 taagttgctc cggcagatgc cagagtttgc ctttatcgag aaactggcgg gaagtgaaga
    11641 tggttttgat ttcagccgcc gtaatagcac tggtcagccc ttttacccca gcagtgtagt
    11701 tcatcattgc gggaatgcgg cgacgcgcga tggccccaaa aatcactgcc gcactgatgc
    11761 ctgcattggg cagcattaag ccgatgcgtt cgccttcaac actgtatttt tcaaggatgc
    11821 gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga gtctggggta aagttgacgt
    11881 cttcgacaca tttcttcccg gctccgaagc ggtacattgc actcagtaaa gattcgtaca
    11941 gagtttcacg cgggcgcacc gccatacgcg cttccatcat tatttgatgc agcatttcgc
    12001 cagcgatttt gcgacggtca cgggcacgcg acgcatccgg catcgccacc tgcgttggag
    12061 gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt aaccagacct ttcaggcggc
    12121 tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat aaccgttgca ccagacttcg
    12181 ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga gcctgtagtg gtgatgcgtc
    12241 cttcagggaa aatcaccact ggtcggccct gttccaccag acgtaccaga tgtttaatag
    12301 ccataggttg cgtcgggtcg agaggaacaa agtcgataaa tgatttcagc caacgcatat
    12361 accactgttg gcttattgag gtgtaaacgg caaacactgg acgcacaggt aaaaacagtc
    12421 caagcaaaat gccatcaata aaagagacgt gattaggcgt aattagaacg cgctcgccct
    12481 tcagtgcctg ggtgtcaccc gtaacgcgaa cgcgatacaa aacacggcac aaatttcgaa
    12541 aaaagctaaa aagcattcca actccctttg ctctgattca gtaaaagcga atggagggag
    12601 attacacgag ataaagaacg cgagcgacag taaattaggt gcgaaaaaaa acctgcgcat
    12661 ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta ccgaataatc tcaccaatca
    12721 atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagcg gtcaatcgca
    12781 acagcgtttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct tacttatttg
    12841 cgtttggctc acattcccac gatgaaaaca cgccactcct tgaaccaacg ggcgttttcc
    12901 gtaacactga aagaatgtaa gcgtttaccc actaaggtat tttcatggcg accataaagg
    12961 atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac
    13021 ccaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct cttagctatc
    13081 acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg
    13141 ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt
    13201 atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa aaagagcgtc
    13261 aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga
    13321 tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc
    13381 gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac ggtgcctggc
    13441 tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc
    13501 actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg
    13561 gtattgcggc caatgaccgg ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac
    13621 aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc tgttataacg
    13681 attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat gtaccgggtg
    13741 agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga
    13801 ccaccgtgcg ttacccaatc gtgacgatgg cgacccaggc tgccgaactg gctttggcgc
    13861 tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg ctggtacgtc
    13921 gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccgc
    13981 agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccgc
    14041 gcctgaccat tatcaaacag aacttctggt aacagcgaac ggctattgta gttggatgac
    14101 attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacctgc cttcacttcc
    14161 ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc gcccgattca
    14221 cataacggtc cggcgacgac ggtttccacc gttggcgcgt gttccagaga acgaccatca
    14281 gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg
    14341 aacccggcat caaccagcac aaagtggcgg ctccccattt gtttgacgct ccgcacctga
    14401 gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc cagtttcaca
    14461 gggtggccca aatggcgggc gatttgctca cgcgcggcat tccacagacc ataataatgt
    14521 tcggtatcaa ccgcctcttc accctgttga taaggaacag aaagcccacc gcccgcagaa
    14581 atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc acacacctgt
    14641 tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac cagctgcaga
    14701 tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg
    14761 ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc cggattaacg
    14821 cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat atcaacagaa
    14881 cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca
    14941 tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca
    15001 cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaagcgc
    15061 aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa ctgtttcagc
    15121 gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattca
    15181 gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt
    15241 ggcataacaa actccagata agtgcttttt tatgattacg ccacatcata aaaagaataa
    15301 aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga ttgcgagaga
    15361 gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt aatgaccgcc
    15421 ggaagcctga ctgaggcggc acacctgcta cacacctcac agccaaccgt cagccgcgaa
    15481 cttgcgcgct ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg tgggcgatta
    15541 catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg gtacggactg
    15601 gatcgaattg tcagcgctgc agaaagtctg cgcgagtttc gccagggaga actgtctatt
    15661 gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc ctttctggca
    15721 cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact tgaagagtgg
    15781 ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc tgcgggaaca
    15841 gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg
    15901 gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat cagcctttcc
    15961 cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca ggttaaacga
    16021 cgcatgatcg tagaaaccca cagcgccgcg tcaatctgcg caatggtacg ggcgggggta
    16081 ggtgtttcgg tggttaaccc gctcaccgca ctggattatg cggcaagcgg tttagtggtg
    16141 cggcggttca gtattgcggt tccgttcaca gtcagcctga tccgccccct gcaccgcccg
    16201 tcatcagcgc tggtgcaggc gtttagtgag catttacaag cggggttacc gaaactggtc
    16261 acttctcttg acgctatttt gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg
    16321 catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctggc accagtaaac
    16381 caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg cgagcaatca
    16441 cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt tcttcaaaaa
    16501 taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg atggaaaatt
    16561 gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta cccagcagcg
    16621 ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca atgtgtaaga
    16681 aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta ttggtacata
    16741 acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc agaatgtccc
    16801 cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa tcgacgctat
    16861 gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct tcatttatca
    16921 aacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca attgttttca
    16981 ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag acaaacgcct
    17041 caacggccaa gtgcccaatc tctattaacg aaaaaagggc cggatgtaca gcacatccgg
    17101 cccgtgaaat cagatgccga tatttctcaa cttctcgcct gccatcagtt tgcgttcgat
    17161 atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtgatgc ccacaaacgc
    17221 aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctgt cgagcagcgt
    17281 caggaaggtc gccgataatc atattcgaca cccagttcgt ggtggtcgaa caggtaatac
    17341 cgaaatcgcg gcatttcagc ggctgaattt cagagcacag gatccacacc actggcgcgg
    17401 cgctcatcgc ataaccggca atacacatca tcgtcatgcc aacagagagc caggacaatc
    17461 cactggaagc cgtaccgtta tcaaactgca tcaggcaata gcccagcacc agagtgccta
    17521 acgccatcac gctgaaacca attttcagag ccggtttacc cctgctttat ctaccgtaaa
    17581 caccgcaata aaggtggcga acataaaggt cagccctacg accagagtcg caatcatctg
    17641 ttgttctgtg gtcgtaaagc ccgccatttt gaagatacgc ggcgcgtagt acatgatgat
    17701 gttcataccg gtaaactgct gcatcgcctg caacaacata ccgagaaaca cagcacgacg
    17761 gacgttacgg ttgatcttaa acagtgccca accgccctgt tttaacttca ggctttcacg
    17821 aatttcgttg agttcttctc gcgctttttc cgacgtatcg cgcagcatac gcaatacttc
    17881 ttccgcctca atatgacgcc ccttttccgc caaccagcgc gggctatttg gcaggaagac
    17941 caccagaata atcagcagaa ctgctggtaa agcaagaacc cccaacattg cgcgccagtt
    18001 accgctataa ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat
    18061 caactggtac atactgatca tcttaccgcg aacgttttca cttgccattt cagaaagata
    18121 cagaggagcg gtgtaagacg cgatcccgac agcaatgccc agccccacac gagcggcgat
    18181 taacatctct acgctggtcg caaaagcgga ccctatagaa ccgagtacaa acaggatggc
    18241 ccccgccatc aggctgtatt tacgccccag gcggaacgac agccaaccat taaacagcgc
    18301 accaattgct gcgccgagca tcatgctact aaccacccat tcctgcaaat gactggtcag
    18361 cacaaagtga tcggtaatga acggcaacgc tccggcgatt acgccgatat caagaccaaa
    18421 taacaatcct gcgaccgcag cagctaccga aacaaacata ttcatacgcc gcgtatcccg
    18481 caaagaacgt ggcgttaaag cagattccgt attgatagta accatttttt cctgccagca
    18541 gagagtaaga catagtgaaa aaatacgtga acaactcacg caggtgtcag gtcggaaaca
    18601 gcataaatat ggattaaatt gctgcgacat gtcgttatgt gatggatatt ccaattttca
    18661 aattaagttg aattattgag attattatta accacctaat tttacagcag ataaaattca
    18721 taaagttcat taattgataa ttaatatgga ttatttcata accatgatat ggattatgat
    18781 gatctacagg tataaaaaac cctgccatgc ggcagggtca taaaagtaag aagaatgaat
    18841 taacgcgcca gccaaccgcc atccacggca atggtataac cattcacata atctgaagcg
    18901 ctggaggcaa ggaacactat cggccccatc aggtcactcg gcagtcccca acgaccagct
    18961 ggaatgcggt cgagaatttc cgcgctacgt tgttcatctg cccgtagttg ttgagtattg
    19021 ttggtcgcca tgtaacccgg agctatcgca ttaacattaa tgttgtgttt agcccattcg
    19081 ttcgccatca atcgcgtcac acccatcacg ccgctttttg atgcggtata agaaggcaca
    19141 cggatcccgc cctggaagga gagcattgac gcgatattga taatcttgcc gccattgcct
    19201 tgcgcgataa agtgtttcgc cgctgcctga gacatgaaga atacgctctt gatattcagg
    19261 ttcatgacat cgtcccagtc tttttcgctg aactcgagag cgtcttcgcg gcgaatcaat
    19321 ccggcgttat tcaccaggat atcaatatga ccaaattcag ctaccgcgcg atccagcagt
    19381 ccaggaatgc catcaatctt tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc
    19441 gccgtgacct gctcgatggt ttcagtcggt tcaacgatgt taatgccaac aatgtcacag
    19501 cccgcttgcg ccagccccaa cgccatcccc tggcccagcc aagtatcaca accagtgacg
    19561 accgcaactt taccttcgag agaaaatgca cttaaaatca taacaatacc ttattcgttt
    19621 atgcccacaa ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt
    19681 tttcaccaac catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg
    19741 accagctcgg ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg
    19801 gttgccccat catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt
    19861 ccatccggcg ctcgtgggtg tgacacggca tggtgttcca caagttacct ggagccagct
    19921 ccgtcagccc catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa
    19981 tagtgcgacg gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccgggg
    20041 tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg
    20101 gagtggcggt atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg
    20161 cgtcgcggtg accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa
    20221 tattgataac acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa
    20281 cttccccgcc aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt
    20341 caatgtggct gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt
    20401 cattgtgcag cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ctctgtctta
    20461 cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac
    20521 tgtttcgttt tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat
    20581 aaaacaatgt tttattttta atttaaggat taaaaaaagt gcagtgtcga tcacgaatat
    20641 gtcatgagaa tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat
    20701 tattcaacag attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg
    20761 tcacgttcaa tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct
    20821 ttgcgggcat tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa
    20881 gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc
    20941 cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata
    21001 agatcgacat cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc
    21061 ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc
    21121 agcacgggta aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg
    21181 ccatcgttta tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga
    21241 tttaaacggg ctaaaccttc tgcactggcg tcagtgcatg gctgttcatc ggtatcaaca
    21301 accaacgtct gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg
    21361 gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga
    21421 cggctgatgc catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg
    21481 aaggcatccc acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt
    21541 gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg
    21601 gcgatgacaa tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta
    21661 agcccagaac cgcaaacgtc attaatagtg attgcagaaa cgctattagg aagaccacct
    21721 ttaatagccg attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga
    21781 attacttcat ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc
    21841 aaactaccaa gttccacggc ggaatgaccc gctaacgcac cacgaaagca gccgataggt
    21901 gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg
    21961 caatagtaaa tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc
    22021 acaaaagaaa tatgcctgag cagcagtcag agacataact ggcacgtaag gtttgcaacc
    22081 actaacccac caatagaggg gtagataggg cgttaatctc ccatacttaa cctggtttat
    22141 ggtaaattgc cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat
    22201 ttggtctaaa gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg
    22261 cacgctcttc cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc
    22321 tctggtcgcc tggcctttaa catattggcc acataaagct ttatgccagt tcatcctctc
    22381 atcgaaaaca tcagcaggtg aagggataac gggcgcggta acacactact atggcaagaa
    22441 gattggtaat ctgattacca cgctgtactt catcgccttt tttgtcgtcg tgttgatata
    22501 tgcagtggca attaccaact cacttacaga acagctggca aagcatatgg ttattgatct
    22561 tcgcatccgt atgttggtga gtctgggtgg gtaatgactc caacttactg atagtgtttt
    22621 atgttcagat aatgcccgat gaccttgtca tgcag
//
LOCUS       DAJTOR010000054        22476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTOR010000054 DAJTOR010000000
VERSION     DAJTOR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.054"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..524)
                     /gene="fsr"
                     /locus_tag="OO249_001846"
     CDS             complement(<1..524)
                     /gene="fsr"
                     /locus_tag="OO249_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5636170.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSVGAVLLAAALVGTSSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLTAVIIAP"
     gene            742..2394
                     /gene="ushA"
                     /locus_tag="OO249_001847"
     CDS             742..2394
                     /gene="ushA"
                     /locus_tag="OO249_001847"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5636171.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(2431..2910)
                     /gene="ybaK"
                     /locus_tag="OO249_001848"
     CDS             complement(2431..2910)
                     /gene="ybaK"
                     /locus_tag="OO249_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5636172.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3114..3908)
                     /locus_tag="OO249_001849"
     CDS             complement(3114..3908)
                     /locus_tag="OO249_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5636173.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4046..4387
                     /locus_tag="OO249_001850"
     CDS             4046..4387
                     /locus_tag="OO249_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5636174.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(4602..7106)
                     /gene="copA"
                     /locus_tag="OO249_001851"
     CDS             complement(4602..7106)
                     /gene="copA"
                     /locus_tag="OO249_001851"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5636175.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPLW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNPALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7368..8300
                     /gene="glsA"
                     /locus_tag="OO249_001852"
     CDS             7368..8300
                     /gene="glsA"
                     /locus_tag="OO249_001852"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5636176.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAVVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8303..9595
                     /locus_tag="OO249_001853"
     CDS             8303..9595
                     /locus_tag="OO249_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5636177.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            9720..10127
                     /gene="cueR"
                     /locus_tag="OO249_001854"
     CDS             9720..10127
                     /gene="cueR"
                     /locus_tag="OO249_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5636178.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10128..10586)
                     /locus_tag="OO249_001855"
     CDS             complement(10128..10586)
                     /locus_tag="OO249_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5636179.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(10583..11500)
                     /locus_tag="OO249_001856"
     CDS             complement(10583..11500)
                     /locus_tag="OO249_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5636180.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11646..12323
                     /gene="fetA"
                     /locus_tag="OO249_001857"
     CDS             11646..12323
                     /gene="fetA"
                     /locus_tag="OO249_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5636181.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12310..13089
                     /gene="fetB"
                     /locus_tag="OO249_001858"
     CDS             12310..13089
                     /gene="fetB"
                     /locus_tag="OO249_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5636182.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13152..14006)
                     /gene="cnoX"
                     /locus_tag="OO249_001859"
     CDS             complement(13152..14006)
                     /gene="cnoX"
                     /locus_tag="OO249_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5636183.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14067..14876)
                     /gene="ybbO"
                     /locus_tag="OO249_001860"
     CDS             complement(14067..14876)
                     /gene="ybbO"
                     /locus_tag="OO249_001860"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5636184.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(14866..15489)
                     /gene="tesA"
                     /locus_tag="OO249_001861"
     CDS             complement(14866..15489)
                     /gene="tesA"
                     /locus_tag="OO249_001861"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR5636185.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            15460..16146
                     /locus_tag="OO249_001862"
     CDS             15460..16146
                     /locus_tag="OO249_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5636186.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16143..18554
                     /locus_tag="OO249_001863"
                     /pseudo
     CDS             16143..18554
                     /locus_tag="OO249_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            18983..22476
                     /locus_tag="OO249_001864"
                     /pseudo
     CDS             18983..22476
                     /locus_tag="OO249_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         5172 a         5899 c         6355 g         5050 t
ORIGIN      
        1 taaggcgcga taatcaccgc cgtcagcaag ggtcccaggg aactgccaaa gttgccgccg
       61 acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg ggctacgcga
      121 gaagattccg gatgaaagac cgatgaactg gtaccgacca gcgccgccgc cagcagaact
      181 gcgccaacac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa gcacatgcca
      241 attggcaacg accacggcat cggatattta tcggtccagt agccgaccac tggttgcagt
      301 agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa tgtcagagaa
      361 aattctgact gaagcagagg ataaatcgcc agaatcagcg attggatcat gtcgttcagc
      421 agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt ggtcgttgac
      481 gcagccgcgc ccgccacagg ctgggtttgt tcactcattg ccataggaaa gtcacttttt
      541 cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa catacctgta
      601 agcgtcgcct gaaggaagtc tcaacgccga atacagaatt tctaatctgg atgcagattt
      661 atcttcaccg gacgcagact tgtctattat gttgcgtcat actatttttc aacacgttga
      721 aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt agcgctgtta
      781 accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa aacctacaaa
      841 attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga atatggcgaa
      901 tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt tgcggctgaa
      961 ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc cgagtctgac
     1021 ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga cgcgatggcg
     1081 atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga aaagtgggcc
     1141 aagttcccgt tgctttccgc gaatatctac cagaaaagta ccggcgagcg cctgtttaaa
     1201 ccatgggcgc tgtttaagcg tcaggatctg aaaattgccg tcattgggct gacaaccgat
     1261 gacacagcaa aaattggtaa cccggaatac ttcactgatg tcgaatttcg taagcccgcc
     1321 gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga cattattatc
     1381 gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc accgggtgat
     1441 gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg tggtcactcg
     1501 caagctccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt gccgggtacg
     1561 ccatgcaaac cagatcaaca aaacggcatc tggattgtgc aggcgcatga gtggggcaaa
     1621 tacgtgggac gggctgattt tgagtttcgt aatggcgaaa tgaaaatggt taactaccag
     1681 ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag cgagcgcgtg
     1741 ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc gccgttccag
     1801 aacaaaggca aagcgcagtt ggaagtgaaa ataggcgaaa ccaatggtcg tctggaaggc
     1861 gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct ggcagcccaa
     1921 atggatcgca ctggtgccga ctttgcggtg atgagtggag gaggaattcg tgattctatc
     1981 gaagcaggcg atatcagcta taaaaacgta ctgaaagtgc agccattcgg caatgtggtg
     2041 gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt cgcacaaatg
     2101 aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtgac gaaagacggc
     2161 aaactcaacg acctgaaaat caaaggcgaa ccggtcgatc cggcgaaaac ttaccgtatg
     2221 gcgacattaa acttcaatgc caccggcggt gatggctatc cgcgccttga taacaaaccg
     2281 ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat ccagaaaagc
     2341 tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca gtaatccgaa
     2401 agtgccggat atttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat cagcaaattt
     2461 ggcatcgagg atcttcgcca gatcgcctgc cgccagttcg atatccagtc cgcgcttgcc
     2521 gccagaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg tcggcagacg
     2581 ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac gctgcgcggc
     2641 catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt ttttaagatc
     2701 cagttgacct gcgaccggcg taacggccac tgccagatgt ttcatatcac cgttcactgc
     2761 caccagtagc gttttgtaga cttgatccgg attcaaacct aattttttga cgacttcatc
     2821 gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg aaatcttgtt
     2881 tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa caaaaaaacg
     2941 aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta agagtattgg
     3001 caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga cggcataata
     3061 ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt tttttagcgc
     3121 aacatttgcg gcaaattccc ttctccatac aggtgtagtg caccgaccgc gaccacatat
     3181 cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg atgcatcagc
     3241 acatcgtaca gcgactgact gaacgtattg ggcagcgtta tatcattatt ttgcggaggt
     3301 gcattcagcc accagctcat catttgttgc agcaaccgtg cgttggtatg ccagtgggtc
     3361 agcgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat ggtaatctgg
     3421 ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc ctgcaataac
     3481 tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt cgcctgaaga
     3541 accatcgcga tttgccacag cggttgagta gaaaagagcg acggagaaat gcccatctcc
     3601 tggctaatgt gctgcagatt ttggagttgc tcctcgttaa tgcgctcttc cagcgcctcg
     3661 caagtaggca aattagcaaa aggcgtatcg ctggtggaaa catccgcctc gacgatcagc
     3721 gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagtcat atcgtggcta
     3781 cccatatgaa tactgccaat cagatgaaaa tggcgattgc cgggaagggt gatatcgatg
     3841 gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac ccggtacaac
     3901 agatccatac gacctccctt tgtgaaatat catgctagcg cgcggtgagg gatggcgcaa
     3961 atggagttgg ggacttgttt aatgtgtttg tatgattcag tatgttcttg catcgctatt
     4021 cacagggaag caacagttaa aaaccatgaa acaggcaaca agaaaaccga cgacacccgg
     4081 agatattctt ctctatgaat atctggaacc gctcgatttg aaaatcaatg agttagcaga
     4141 gttgctgcat gttcatcgta atagcgtcag tacactgatc aataataatc gtaaactcac
     4201 cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt tttggctaaa
     4261 cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc aggaagaatt
     4321 gggacggatt gaaacagtgg ctgaatattt ggcacgccgt gaagagcgtg caaaaaaggt
     4381 cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttca cgccgcatcc
     4441 ggcatccgag caccatagcc tgatgcgacg cttacccgtc ttatcaggcc tacaaacctg
     4501 tgcctgaacc gtaggccgga taaggcgttt acgccgcatc cggcagtcat gcgtcgatgc
     4561 caaatgcgcc gccctaaagc agcgcatccg caatgacgta cttattcctt cggtttaaac
     4621 cgcagcaacc ggttggcgtt actcactacg gtaatcgacg agagcgccat tgccgctccg
     4681 gcaactaccg ggttaagcag tgttccagta aacggccaca aaataccagc ggcgaccgga
     4741 ataccaatgc tgttgtagat aaacgcaccg agcaggttct gcttcatatt gcgcagcgtt
     4801 gcgcgggaaa tcgcgagcgc atctgcaaca cccatcaggc tatggcgcat cagggtaatc
     4861 gccgcggttt caatggcaac atcactgccg ccacccatcg caataccgac atccgcctga
     4921 gccagcgctg gcgcgtcgtt aatgccgtcg cccaccattg ccacctgacg tccttcactt
     4981 tgcagacgtt tgatcgcttc ggctttaccg tctggcagca ccccggcgat tacctcatca
     5041 atccctgctt ctttggcgat cgcattggcg gtggttgggt tatccccggt caacatcacc
     5101 agacgatatc ccgctttatg caggcgttgc agcgccgcca cgctatcact acgcaacgga
     5161 tcgcgtacag ccagcagggc tactactttc ccgtcaatcg ccagcagcac aggcgttgct
     5221 ccttgcgatg cctgagctga aatatcggct tcaatcgctt tagtatcgac ttgctggtca
     5281 tttaacagcg ccggattacc cagcaataac gcatgacctt cagcttcacc gcttacgccc
     5341 agcccgcgca atgtgcggaa accgttgacc tgcggcagct gcatatcgcc tgctttatcg
     5401 aggatcgctc gtgccagcgg atggctggaa ccttgctcca gcgccgccgc cagacgcaat
     5461 gcttgcgctt catcaacatc agcaaatgtt tttactgcga caacctgcgg cttcccttcg
     5521 gtcagcgtcc cggttttatc gaacacgacg gtatcgagtg tactggcgcg ttgcagcgcg
     5581 tcggcgtccc gcaccagcac gccaaactca gccgcccgcc cgacgccgga aataatcgac
     5641 attggcgtcg ccagccccag cgcacacgga caggcaataa tcagcaccgt ggtggctatc
     5701 accagggtat agacaatctg cggtgccgga ccaaagaaat accagattgc tgcactgatc
     5761 agcgcaatca ccactactac cggcacaaat acggctgaga ttttatccgc cagctgaccg
     5821 atttctggct tgctgctctg ggcctggcgc accatgcgaa taatccttga cagcgtggta
     5881 tggctgccaa ccgcactggc gcgaaatagc acgctaccgt cctgtaccac tgtcccggca
     5941 tggacgcttt cgccttcgcc tttttgctgc gggattggtt cgcccgtcag catcgcttca
     6001 tccagccatg cttcgccctg ggtaatctcg ccatctaccg gcacgcgatc gccggtcgtc
     6061 aggcgcagta acatccctgg ctgaacttcc gccagcggca cgcttttttc accctctgcg
     6121 gtgaccactc gcgccgtcgg tggggttaaa tcgagtaatt tctccagcgc cttagaagaa
     6181 cgctgacgcg cgcgcgcttc cagcatatgg ccgagattaa tcagaccgat aatcatcgca
     6241 ctggcttcat agtaaagatg ccgcgcttcc atcgggaacc acagcggcca caggttgacg
     6301 ctcatcgaat agagccacgc cacacccgta cccagcgcca ccagcgtatc catcgtcgcc
     6361 gcaccgttca gcaggctttt ccacgcgctg cggtaaaaat gcccgccggc gaaaaccatt
     6421 accgccaggg ttatcaggcc gataactaac cacaggctgc ggttgtcagc ggtgaccatc
     6481 atgttatcgc cgatcatccc ccagaccatt accggaatac ccaccgccag tgcgacaatc
     6541 gcctgccagc ggaagcgctt catcgtagcg acggcggttt cctgctggcg ctcgcggcgt
     6601 ttcgcgtcat cttcaatcgc ttccgcgccg tagcccgctt tttccaccgc ctgcactaaa
     6661 tcttgtgggg aggcactgcc catcaccagc gcagtacgct ccgccaggtt tacccgtgcc
     6721 tgagtgacgc ccggtacgct ttgcagcgca ttttgtacgc gggtgacaca gctggcgcag
     6781 ctcatgccgc tcagcagcaa ctgctggctg tcatcgtcat cggcggtcgc tgccggaagc
     6841 gcctcagaaa ccgctgtcag tgcttccgac gggattgatg actccgccag cggtttagcc
     6901 tttgggtggc ttacagatgc gtcataaccc gcttgtttga tggtttcgat cagctgttct
     6961 gcactggcag tcccggtaac gtgcgcttca gtgatagaca catccgcttg ttccacatcc
     7021 gggcgctgtt cgagactttc tttcacgcgt ttaacgcagt gaccgcagga caggccgtcc
     7081 agggtcaggt cgatagtttg tgacataaaa cactccttta agacagtttt gactggctgt
     7141 gataaaggtt aaaccttcca gcaaggggaa ggtcaagaaa ttaataaacc aggcgggtaa
     7201 aagtccgtaa agattaaaaa atcggctcga tttgcatcag gattagacat ttatttcttt
     7261 gttttcctgt agttaagttg ctggtgctaa gttaaatcag gatgcctgaa aatcggcacc
     7321 ggggtgagga attacctccc gcatctataa aaaggagtta acaaaagatg ttagatgcaa
     7381 acaaattaca gcaggcagtg gatcaggctt acacccaatt tcactcactt aacggcggac
     7441 aaaatgccga ttacattccc tttctggcga atgtaccagg tcaactggcg gtagtggcta
     7501 tcgtgacctg cgatggcaac gtctatagtg cgggtgacag tgattaccgc tttgcactgg
     7561 aatccatctc gaaagtctgt acgttagccc ttgcgttaga agatgtcggc ccgcaggcgg
     7621 tacaggacaa aattggcgct gacccgaccg gattgccctt taactcagtt atcgccttag
     7681 agttgcatgg cggcaaaccg ctttcgccac tggtaaatgc tggcgctatt gccaccacca
     7741 gcctgattaa cgctgaaaat attgaacaac gctggcagcg aattttacat atccaacagc
     7801 aactggctgg tgagcaggta gcgctctctg acgaagtcaa ccagtcggaa caaacaacca
     7861 acttccataa ccgggccata gcctggctgc tgtactccgc cggatatctc tattgtgatg
     7921 caatggaagc ctgtgacgtg tacacccgtc agtgctccac gctcatcaat actgttgaac
     7981 tggcaacgct tggcgcgacg ctggcggcgg gtggtctgaa tccgctgacg cataaacgcg
     8041 ttcttcaggc cgacaacgtg ccgtacattc tggccgaaat gatgatggaa gggctgtatg
     8101 gtcgctccgg tgactgggcg tatcgcgttg gtttaccggg caaaagcggt gtaggtggcg
     8161 gtattctggc ggtcgtccct ggcgtgatgg gaattgccgc gttctcacca ccgctggacg
     8221 aagaaggtaa cagtgttcgc ggtcaaaaaa tggtggcatc ggtcgctaag caactcggct
     8281 ataacgtgtt taagggctga tcatgatgaa cacggaaggt aataacggta acaaacccct
     8341 cggtctatgg aatgtcgttt ccatcggtat tggggcaatg gtgggggcgg ggatcttcgc
     8401 gctgctgggg caggctgcgt tgctaatgga agcctcgacc tgggtcgcct ttgcttttgg
     8461 cggtattgtg gcgatgtttt ccggttatgc ctatgcgcgt ctgggggcga gctatcccag
     8521 caatggcggc attatcgact tctttcgtcg cggattaggc aacggcgtct tttcgctggc
     8581 gctctcgtta ctgtacctgt tgacgctggc ggtgagcatc gccatggtcg cccatgcttt
     8641 tggcgcttat gccgtgcagt ttttgcatga aggcagccag gaggagcacc ttattttgct
     8701 ctacgcgttg gggagcattg cggtgatgac gcttttcaac tccttaagca accatgcggt
     8761 agggcggctg gaagtgatcc tcgtcggcat taaaatgatg atcctgttat tgctgattat
     8821 tgccggtgtc tggtcgctgc aaccagcgca tatttccgtc tctgcgcccc ccagctccgg
     8881 tgcgttcttc tcctgtattg ggataacttt ccttgcctat gcgggctttg gcatgatggc
     8941 gaacgcggcg gataaagtga aagatccgca ggtcattatg ccacgggcgt ttctggtggc
     9001 gattggcgtt accacgttgc tttatatctc gctggcactg gttttgctta gcgatgtatc
     9061 ggcattagag ttagaaaaat atgccgatac cgccgtagcg caggctgctt ctccgctgct
     9121 cggacatgtg ggttatgtga tcgtcgtcat cggcgcttta ctggcgacgg cttcagccat
     9181 taacgcgaac ctgttcgccg tgtttaacat catggacaac atgggcagcg aacgcgaact
     9241 gccgaagcta atgaataaat ccctgtggcg gcagagtacc tggggcaaca tcattgtcgt
     9301 ggtgttgatt atgctgatga cggcggcact gaatttaggc tcactcgcca gcgttgccag
     9361 cgccaccttt ttgatttgct acctggcggt gtttgtggtg gcgatccgcc tgcgtcatga
     9421 tattcacgcc tcgttgccga ttcttatcgt tggtacgttg gtgatgttgt tggtgatcgt
     9481 tggctttatc tacagtctgt ggtcccaggg tagccgtgcg ttgatatgga ttattggctc
     9541 actcttactc agccttattg tggcaatggt catgaagcgc aataaaaccg tataacatct
     9601 ctctgtgcgc agtacttcct gtattattgt ggtggcggtc gatattcgca ctggcaaaaa
     9661 aacgtgcttg aatatctgtt gaaacccttt aacaaagcac aggaggcgtt gcgcgaacga
     9721 tgaacatcag cgatgtagca aaaattaccg gcctgactag caaagccatt cgcttctatg
     9781 aagagaaggg gctggtgacg ccgccgatgc gcagcgaaaa cggttatcgc acctacacgc
     9841 agcagcatct caacgaactg accttactgc gccaggcacg gcaggtgggc tttaacctgg
     9901 aagagagcgg cgagctggtg aatctgttta acgacccgca gcggcacagc gccgacgtca
     9961 aacggcgcac gctggagaag gtggcggaga tcgaacgaca cattgaggag ctgcaatcca
    10021 tgcgcgacca gctgctggca ctggcgaatg cctgccctgg cgatgacagc gccgactgcc
    10081 cgattatcga aaatctctcc ggctgctgtc atcatcgggc agggtgatta agacgagacg
    10141 gctcggatat gtagggttat cccttctatc gcaatgactt caacatgcgt acctgcaccg
    10201 agatcctcgc tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg accgcgcccg
    10261 ttgaccagcg gagattccag cacaaaacgt cggccaatca gctgctgccc gcgctggttt
    10321 aaatgactat cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt ccaccacagc
    10381 caggcggcga gcagcgtcag gatggcaaac attacccctt gccactccca acccagcggc
    10441 accagccaga ccaccaggcc ggtaatcacc gctgccacgc cgctccacaa caaataacca
    10501 tttccgccca gcatctcggc tgccagcagc aaaccgccga gactgagcca gaaaatatgt
    10561 ggatgaacga ccattaactc catcatggct gagtccgttt gttggcgctg tctttcacca
    10621 gttcggcaat cccggcaatc gaccccatca ggctgctggc ctctaatggc atcatcacta
    10681 ctttgctgtt actggaggaa ccgatctgct gtaacgcttc ggtgtatttc tgcgctacga
    10741 agtagttcac cgcctgaata tcaccggagg cgatagcttc agacaccatt ttggtggcgc
    10801 gggcttctgc ttcggcggaa cgttcacgcg cttcagcctg taaaaacgcc gactgacgtt
    10861 cgccttccgc tttcaggatt tgcgactgtt tttcaccttc ggctttgagg atttccgcct
    10921 gacggatccc ttccgcttca agaatgtaag cgcgtttggt acgttccgct ttcatctgcg
    10981 cgttcattga agagataagc tctgccggtg ggcgcacgtc gcgaatttca atacgggtga
    11041 ctttaatccc ccacgggttg gtggcttcat cgacaatacg cagcaggcgt gagttgatgc
    11101 tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc caacacggta cggatgttag
    11161 tcatggtcag gttgatgatc gccagctcca gattgctgac ttcataggca gcgcgtggcg
    11221 cgtcaatcac ctgaataaaa cagactgcgt cgatggtaac gttggcgtta tctttcgaga
    11281 taacttcctg ggaagggata tcgagcactt gctccatcat attgatcttg cgaccaatgc
    11341 gatccataaa cggcaccacc agactgagcc ccggctgtaa cgttttggta tagcgaccaa
    11401 aacgttctac cgtccattga tagccctgcg gcacgatttt gacgcccgcg ccgacaatga
    11461 ccagcgcgac aaaaatgaga atcgggataa agataagcat cggaaaaacc tcctgttgta
    11521 ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat tatacctgat tactgaaaga
    11581 gagttccccc ttattcctgc gaaggataaa ctgtttttag taaaaatcag aaaaagggaa
    11641 cagcgatgca ggaaaatagt cctttgcttc agctacaaaa cgtaggatat ctggcgggtg
    11701 atgcgaagat tcttaataac atcaattttt cgctgcgtgc tggcgaattt aagttaatta
    11761 ccggtccttc tggttgtggc aaaagtacgc tgctaaaaat agttgcttca ttgatcagcc
    11821 caaccagcgg aacgttactg tttgaaggtg aggatgtcag cacactaaag ccagaaatct
    11881 accgccaaca agtctcttac tgcgcccaga caccgacgct gtttggcgat acggtatacg
    11941 ataatctgat ctttccttgg cagatccgta accggcagcc tgacccagcc atttttctcg
    12001 attttctcga acgcttcgcc ttgccggaca gcattttgac gaaaaatatc gccgagctat
    12061 ctggtggtga aaaacaacgc atctcactga ttcgtaacct gcaatttatg ccgaaggttt
    12121 tattgctgga tgaaatcacc agtgcgctgg atgaaagtaa taaacataac gttaatgaga
    12181 tgatccatcg ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca cacgataaag
    12241 acgaaattaa tcatgcggat aaagtgatta cactgcaacc gcatgccgga gaaatgcagg
    12301 aagcacgcta tgaactcgca taatattact aacgaatcat tggcactggc attaatgctg
    12361 gtggtggtgg caatcttaat tagccataaa gaaaaactgg cgctggagaa agatattttc
    12421 tggagcgtcg ggcgcgcgat aattcagctg attattgtcg gctatgtgct gaagtatatt
    12481 ttcagcgtgg atgatgccag cctgacatta ttgatggtgt tatttatctg ctttaatgcg
    12541 gcgtggaacg cgcaaaaacg cagtaaatat attgctaaag cttttatctc atcgtttatt
    12601 gccattacgg ttggggcggg aattaccctg gcggtgctga ttctctcagg gtcgattgaa
    12661 tttatcccga tgcaggtgat ccctatcgcc gggatgattg ccggtaacgc catggtagcg
    12721 gtggggttgt gttacaacaa tttagggcaa cgggtcatta gcgaacagca acagattcag
    12781 gagaaactga gtcttggcgc gacgccgaag caggcttcag cgatattgat tcgcgacagt
    12841 attcgcgcgg ctttaattcc gacggtcgat tcagcaaaaa cggttggctt agtgagttta
    12901 ccaggaatga tgtccgggct gatatttgcc gggattgatc cggtgaaggc gattaaatat
    12961 cagattatgg tgacctttat gctgctctca accgccagct tgtcgaccat tattgcctgc
    13021 tatttaacct atcgtaagtt ttataattcg cgccaccagt tggtggtgac gcaattgaag
    13081 aagaaatgat gcgatgccgg atgcgcagca tcgcatccgg cgttgtggtt catgtgccga
    13141 acaacggaat atcaatacaa caatgcatac agctggcggc gatacttcga cgccagtgca
    13201 tcacccgtac ccagcgcagc gaggatctcc tggaacgttt tacgcgtctg accgtctgcg
    13261 gcggtgagat ctttacgcag atgcccgaac agcaactcca gcgcctcttc attgcgccca
    13321 acctgatgca gttgcagcgc cagttgcgtc gccagtgcgg catcttctgg attctccgcc
    13381 acctgctgtt gcaactgttg aatttccggc gtatcagccg cctgcttcag cagttcgatt
    13441 tgcgccacca gcccctggta gcgggtgtcc tgatcctgca acggaatggt tttcagcacc
    13501 gcttccgcat cttcagaacg gttcagcgca atcagcgttt ctgccagcag caggccgatc
    13561 tccccgttct gattcgacaa ctgccaggcg tctttcagca atggcagggc atcggtgtaa
    13621 ttgccttcct gcatcagttg catcgcctgc tgcgctttca gctcttcttc gcgcggcagc
    13681 actttatcca gcagggcgcg gatcgcctct tccggttgcg gcccctggaa gccatctacc
    13741 ggttgcccgt tctggaacag atacacggtc ggaatcgcgc gcagaccaaa ctgggcggca
    13801 atcatctgct ccgcgtcgca gtccagcttc gccagaataa attgcccgtt gtactgcgcc
    13861 gcgaggcttt ccaggatcgg ggttaactgc aaacagtgct ggctacgttc agaccaaaaa
    13921 tagaacagca ccggagtggt catcgactgt tcaagaacct gttgcaggtt agattcgtta
    13981 atgttgacaa tattttctac ggacatggag tcgctctctg ttgtcgatat tttctttgac
    14041 atgggggctt aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat cacgcgccca
    14101 ggcagcaggc gcttgagcac cattactgcc caggtcacca gcgttaccgg atagcgcatc
    14161 ttcggcttct cgctaataaa agcatggcgc actttgtcga ccaccgcttc cggtcccaac
    14221 gtaaagcggg cggcgatgcc gggattttcg actggtttat cactttgcgt ctggttgacg
    14281 ttgtcggtga agcgagtacg aatgggaccg ggttcgatca ggctgacttt aattccgctg
    14341 tggcgtagct ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt actggccgcg
    14401 taagcgccac gacccggcgt ggagattaat cccatcaccg atgatgtcat cacaatacgc
    14461 ccttcaccgt gcggtaacat cgcgggtaac aggcgcatgg tgagctggtg tgcgccgaaa
    14521 aagttggcgg aaaactgctg ttccatctgc gcacggctga tggtggaaag ggggccatac
    14581 atgccgaatc cggcattgtt aaagatccca tacagacaat tatcggtcag ggcgatcacc
    14641 tcgtctgctg cgcgatcaac actttctggt gaatccagat cgatcaacac gccggtaaat
    14701 cccatgttgt tcatacgctc aacatcatcc ggttttcggc aacctgccag cacatgaaaa
    14761 ccctggcgtt ttaattcgag cgcgctttcc aggccaattc cactggaaca tccggtaatt
    14821 aagaccgatt tttgcataac tttacctgtc aggatctccg ttgctttatg agtcatgatt
    14881 tactaaaggc tgcaactgct tcgccatcca gtcggcaata aacggctggg cgtcgcggtt
    14941 gggatgaata ccgtcatcct gcatccattg tggcttgagg tagacctctt ccataaaaaa
    15001 gggcagcagc ggaacatcaa actctttggc gagtttgggg taaatggcgc taaaggcttc
    15061 attataacgg cgaccatagt ttgcaggcag acgtatttgc attaacaatg gttcagcgtt
    15121 ggcggctttg acatcctgca aaatctggcg cagcgtttgc tcggtttgct gtggctgaaa
    15181 accacgcaaa ccgtcattgc cgcccagttc aaccagcacc cagcgcggct gatgctgttt
    15241 cagcagagcc ggaaggcgcg ccagtccttg ttgcgaggtg tcgccgctga tgctggcatt
    15301 aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg ccgcgctggc
    15361 agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt ccgctgcggc
    15421 ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga aaacattgtt
    15481 gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc catcctcacc
    15541 ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga gtcgggatcg
    15601 ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag tggcgaagtg
    15661 agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa gttgcgcgcg
    15721 aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa cgcgctggaa
    15781 aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg taacggggcg
    15841 aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc ggcacagctt
    15901 tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg acctgatgtg
    15961 ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa aattgccgac
    16021 ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac ccacgacctg
    16081 caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt gcaggaggaa
    16141 gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta attgtctggc
    16201 tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc gatcgcatgg
    16261 agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg ttgcgcagtt
    16321 cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg aaagtcggca
    16381 agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg gcgaacgtca
    16441 aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc cctggcctga
    16501 aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt aacctgaaaa
    16561 cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa gtgattcagg
    16621 aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg aatctggcgg
    16681 atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt tataaattcg
    16741 gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag cttaaacccg
    16801 aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg atggaacgct
    16861 cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg gcagcggtgg
    16921 cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc ctcaaaacgc
    16981 tgggggcagg gcgaacgcaa ctgcgtaagc taatcgtcgg tcagtggttg atggtgctga
    17041 cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg ttgatggtgc
    17101 tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg tggctgtggg
    17161 cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac cgcttgttgc
    17221 tggcaacgca gcctttacgc gtattacgta atgatgtggt agcgaacgtc tggccgctga
    17281 agttttatct gccgattgtc agtatggtgg ttgtgctgct gctcgccgga ttaatgggtg
    17341 gcagcatgct gctttgggcg gtgctggcgg gcgcagtagt actggctttg ctgtgcggtg
    17401 tgctgggctg gatgcagctg aatgtacttt gccgcatgac gctgaaatcg ctacctctgc
    17461 gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag ctttcggcat
    17521 tttcgctctc ctttatgctg ctggcgctgt tgctggtatt gcgttgcgat ctgctcgacc
    17581 gctggcagca gctaccgcca gaaagcccga actacttttt aattaacatc gccacagaac
    17641 aggtagcacc gctaaaagcg ttcctcgcgg aacatcatat agtcccggaa tcattttatc
    17701 cggtagtgcg ggcgcggctg acggcgatta acgataagcc gacagaaggt aatgaagatg
    17761 aggcgcttaa tcgcgaactc aatcttacct ggcaaaatac gcggcccgat cataatccga
    17821 ttgtcgccgg taactggccg ccaaaagccg atgaagtgtc gatggaagag gggctggcga
    17881 aacgcttaaa cgttgccctc ggtggtaccg tgacttttat gggcgatacc caggagttcc
    17941 gcgctaaagt gaccagcctg cgcaaagtgg actgggaaag tctgcggcct aatttctatt
    18001 ttattttccc ggaaggggca ttagacgggc aaccgcagag ctggcttacc agtttccgct
    18061 gggagaatgg caacggcatg ttgacgcaac tcaaccgcca gttcccgacc attagcctgt
    18121 tagatattgg cgcgatttta aaacaggtcg gttaggtgct ggagcaggta agtcgggcgc
    18181 tggaggtgat ggtggtgctg gtcaccgcct gcggtatgtt gctgttgctg gcacaggtgc
    18241 aggtgggaat gcgccagcgt catcaggagc tggtggtatg gcgcacactc ggtgcgggga
    18301 aaaaactgct gcgcacgaca ttgtggtgtg agttcgccat gcttgggttt gtttccggcc
    18361 tggtggccgc aattggtgcg gaaacggcac tggcagtgtt gcaggcgaaa gtgtttgatt
    18421 tcccgtggga gccagactgg cgattgtgga ttgttctgcc gtgcagcggt gcgctgctgc
    18481 tgtcgctttt cggcggctgg ctgggtgcgc gactggttaa gggtaaggcg ctgttcaggc
    18541 agtttgcggg gtgatgagag taaataaggg gcgttctggt tgaatggaac gccttgttta
    18601 aattaattct acaatatgtt tcattgatga tgttattgaa ttggtgattt cctatctttc
    18661 tattgctgat attaatctga attggtggag tttaataatt ttaaatatag ccctacaaaa
    18721 ataactcaga ctaataaaat cataaatcat atgcgttgaa tggatattat ccatatagtg
    18781 aatttgttga tgatgaattc atctgtgcta aaaattttag tttaataaaa tattgaaagt
    18841 gacctgtaat aacagttatt gttgattgag aacaaataag tttatgtgaa aaatatataa
    18901 atacattagc tggtcatgtg tgtcatttta tttttttgtt gctaacacag ggatatgaac
    18961 aataactaaa agggcacttt atatgagcgg aaaaccagcg gcgcgtcagg gagatatgac
    19021 tcagtatggc ggtcccattg tccagggttc ggcaggtgta agaattggcg cgccaactgg
    19081 cgtggcgtgc tcggtgtgtc cgggcgggat gacctcgggc aacccggtga acccgctgct
    19141 cggtgcaaaa gtacagcccg gcgagacgga ccttgcgctg cccgacccgc tgccgttcat
    19201 tctctcccgc acctacagca gctaccggac gaagacgcct gcgccagtgg gaactttttg
    19261 gccccggctg gaaagcgcct tctgatatcc gcttacagat acgcgatgat gcactggtac
    19321 tcaacgacaa cggcgggcgg agcattcact ttgagccgct gctgccgggg aaggctgtgt
    19381 acagccgcag tgagtcaatg tggctggtgc gtggcggtaa ggcagcgcag ccggacggcc
    19441 acacgctggc acgactgtgg gcgtcgctgc cgccggatat ccggttaagc ccgcatcttt
    19501 acctggcgac caacagcgca caggggccgt ggtggatact ggggtgatct gagcgggtgc
    19561 cgggtgctga ggacgtactg ccagcgccgc tgccgccgta ccgggtgctt accgggatgg
    19621 cggaccgctt cgggcggacg ctgacgtacc ggcgtgaggc cgcaggcgac ctggccgggg
    19681 aaatcaccgg cgtgacggac ggtgccgggc gggagttccg tctggtgctg accacacagg
    19741 cgcagcaggc ggaagaggcc cggcagcagg cggtcgtcgc cggagcaaaa cgaccggaca
    19801 ttcctgattc actgccggag agcacagagt acggccccga caggggtatc cgcctttcgg
    19861 cggtgtggct gatgcacgac ccggcatacc cggagagcct gcccggtgcg ccactggcgc
    19921 ggtacacgta tacggaagcc ggtgaactgc tggcggtata tgaccgcagc aatacgcagg
    19981 tgcgcgcttt cacgtatgac gcgcagcacc cgggccggat ggtggcgcac cgttacgcgg
    20041 gaaggccgga gatgcgctac cgctacgacg atacggggcg ggtggtggag caactgaacc
    20101 cggcagggtt aagctaccgc tatctttatg agcaggaccg catcaccgtc accgacagcc
    20161 tgaaccggcg tgaggtgctg catacagaag gcggggccgg gctgaaacgg gtggtgaaaa
    20221 aagaactggc ggacggcagc gtcacgcgca gcgggtatga cgcggcagga aggctcacgg
    20281 cgcagacgga cgcggcggga cggcggacag agtacggtct gaatgtggtg tccggcgata
    20341 tcacggacat caccacaccg gacgggcggg agacgaaatt ttactataac gacgggaacc
    20401 agctgacggc ggtggtgtac ccggacgggc tggagagcag ccgggaatat gatgaaccgg
    20461 gcaggctggt atcggagaca tcgcgcagcg gggagacagt acgctaccgc tacgatgacg
    20521 cgcacagtga gttaccggcg acgacaacgg atgcgacggg cagcacccgg cagatgacgg
    20581 cggttcaccg cgaggaaggt atcagccttt accgccacta tgacaaccgt ggccggttaa
    20641 cctcggtgaa agacgcacag ggccgtgaaa cgcagtatga gtacaacgcc gcaggcgacc
    20701 tgactgccgt tatcaccccg gacggcaacc ggagcgagac acagtacgat gcgtggggaa
    20761 aggcggtcag caccacgcag ggcgggctga cgcgcagtat ggagtacgat gctgccggac
    20821 gtgtcatcag tctgactaac gagaacggca gtcacagcga cttcagctac gatgcgctgg
    20881 accggctggt acagcagggc ggctttgacg ggcggacgca acgttatcat tatgacctga
    20941 ccggaaaact cacgcagagt gaggatgagg ggcttgtcac cctctggcac tacgatgcgt
    21001 cggaccgcat cacgcaccgg acggtgaacg gcgccccggc agagcagtgg cagtatgacg
    21061 gccacggctg gctgagagaa atcagccacc tgagcgaagg ccaccgtgtc gctgtccact
    21121 acggctacga cgataagggc cgcctgacgg gcgaacggca gacggtggag aacccggaga
    21181 cgggggaact gctgtggcag catgagacga aacacgccta caacgaacag gggctggcaa
    21241 accgcgtcac gccggacagc ctgccgccgg tggagtggct gacctacggc agcggttacc
    21301 tggcgggcat gaagctgggc gggacgccgc tggtcgagta tacgcgggac aggctgcacc
    21361 gtgagacggt gcgcagcttc ggcagcatgg caggcagtaa tgccgcatat gaactgacca
    21421 gcacatacac ccccgcaggc cagttacaga gccagcacct gaacagcctg gtgtatgacc
    21481 gtgactacgg gtggaatgac aacggcgacc tggtgcgcat cagcggtccg cgacagacgc
    21541 gggaatacgg ctacagcgcc acgggcaggc tggagagcgt gcgcaccctc gcaccggacc
    21601 tggacatccg catcccgtat gcgaccgacc cggcgggaaa ccggctgccg gacccggagc
    21661 tacacccgga cagcacgctc acggcgtggc cggataaccg catcgcagag gatgcgcact
    21721 atgtttacca ctatgatgaa tacggcagac tgacggagaa gacggaccgc atcccggcgg
    21781 gtgtgatacg gacggacgac gagcggaccc accactacca ctacgacagc cagcaccgcc
    21841 tggtgttcca cacgcggata cagcatggcg agccactggt cgagagccgc tacctctacg
    21901 acccgctgag ccgccggacg gggaaacggg tgtggcgtcg cgagcgtgac ctgacggggt
    21961 ggatgtcgct gtcgcgtaaa ccggaggtga cgtggtacgg atgggacggc gaccggctga
    22021 cgacggtaca gaccgacacc acgcgtatcc agacggtata ccagccgggg agcttcgcgc
    22081 cgctcatccg catcgaaaca gacaacggtg aacgggagaa agcgcagtgc cgcagcctgg
    22141 cggagaagtt acagcaggaa gggagcgaag acggtcacgg tgtggtgttc ccggctgaac
    22201 tggtggggct gctggacagg ctggaggggg aaatccgggc aaactgcgtg agcagtgaaa
    22261 gccgccagtg gcttgcgcag tgcgggctga cggtggagcg gctggcggca cagatagagc
    22321 cggtttattt gcctgagcga aaaatacatc tttatcactg tgaccaccgg ggcctgccgc
    22381 tggcgcttat cagtgaagac ggcaatacgg cgtggagcgc ggaatatgat gaatggggca
    22441 accagcttaa tgaggagaac ccgcatcacc tgcacc
//
LOCUS       DAJTOR010000055        22350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTOR010000055 DAJTOR010000000
VERSION     DAJTOR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.055"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1298
                     /locus_tag="OO249_001865"
     CDS             <1..1298
                     /locus_tag="OO249_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5636187.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPY
                     VRILTQDEIDVRIKRFWRYLDGTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEIPPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
     gene            1270..2133
                     /locus_tag="OO249_001866"
     CDS             1270..2133
                     /locus_tag="OO249_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5636188.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(2347..3129)
                     /locus_tag="OO249_001867"
     CDS             complement(2347..3129)
                     /locus_tag="OO249_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5636189.1"
                     /translation="MICRFIDTHCHFDFLPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(3126..4199)
                     /locus_tag="OO249_001868"
     CDS             complement(3126..4199)
                     /locus_tag="OO249_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5636190.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGCYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(4321..4482)
                     /locus_tag="OO249_001869"
     CDS             complement(4321..4482)
                     /locus_tag="OO249_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5636191.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="OO249_001870"
                     /pseudo
     CDS             complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="OO249_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
     gene            complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="OO249_001871"
     CDS             complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="OO249_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5636192.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(7287..7964)
                     /gene="yjjG"
                     /locus_tag="OO249_001872"
     CDS             complement(7287..7964)
                     /gene="yjjG"
                     /locus_tag="OO249_001872"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5636193.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHLEQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="OO249_001873"
     CDS             complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="OO249_001873"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5636194.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKNFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(8394..8807)
                     /gene="holD"
                     /locus_tag="OO249_001874"
     CDS             complement(8394..8807)
                     /gene="holD"
                     /locus_tag="OO249_001874"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5636195.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            8910..9941
                     /gene="rsmC"
                     /locus_tag="OO249_001875"
     CDS             8910..9941
                     /gene="rsmC"
                     /locus_tag="OO249_001875"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5636196.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            10209..10295
                     /locus_tag="OO249_001876"
     tRNA            10209..10295
                     /locus_tag="OO249_001876"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10243..10245,aa:Leu,seq:cag)
     gene            10324..10410
                     /locus_tag="OO249_001877"
     tRNA            10324..10410
                     /locus_tag="OO249_001877"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10358..10360,aa:Leu,seq:cag)
     gene            10446..10532
                     /locus_tag="OO249_001878"
     tRNA            10446..10532
                     /locus_tag="OO249_001878"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10480..10482,aa:Leu,seq:cag)
     gene            complement(10571..10807)
                     /locus_tag="OO249_001879"
     CDS             complement(10571..10807)
                     /locus_tag="OO249_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5636197.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            10948..11736
                     /gene="fhuF"
                     /locus_tag="OO249_001880"
     CDS             10948..11736
                     /gene="fhuF"
                     /locus_tag="OO249_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5636198.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(11774..12450)
                     /gene="bglJ"
                     /locus_tag="OO249_001881"
                     /pseudo
     CDS             complement(11774..12450)
                     /gene="bglJ"
                     /locus_tag="OO249_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(12408..13133)
                     /locus_tag="OO249_001882"
     CDS             complement(12408..13133)
                     /locus_tag="OO249_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5636199.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            13752..14522
                     /locus_tag="OO249_001883"
     CDS             13752..14522
                     /locus_tag="OO249_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5636200.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAIITFFASTAAMYIRQLLAQ
                     HHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            14513..14986
                     /locus_tag="OO249_001884"
     CDS             14513..14986
                     /locus_tag="OO249_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5636201.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTRGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            15094..15633
                     /gene="dnaT"
                     /locus_tag="OO249_001885"
     CDS             15094..15633
                     /gene="dnaT"
                     /locus_tag="OO249_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5636202.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            15636..16373
                     /gene="dnaC"
                     /locus_tag="OO249_001886"
     CDS             15636..16373
                     /gene="dnaC"
                     /locus_tag="OO249_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5636203.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            16422..16916
                     /locus_tag="OO249_001887"
     CDS             16422..16916
                     /locus_tag="OO249_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5636204.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            17170..19461
                     /gene="opgB"
                     /locus_tag="OO249_001888"
     CDS             17170..19461
                     /gene="opgB"
                     /locus_tag="OO249_001888"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5636205.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(19600..20622)
                     /gene="lgoD"
                     /locus_tag="OO249_001889"
     CDS             complement(19600..20622)
                     /gene="lgoD"
                     /locus_tag="OO249_001889"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR5636206.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGLQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            20761..21675
                     /locus_tag="OO249_001890"
     CDS             20761..21675
                     /locus_tag="OO249_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5636207.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            21890..>22350
                     /locus_tag="OO249_001891"
     CDS             21890..>22350
                     /locus_tag="OO249_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5636208.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKV"
BASE COUNT         5521 a         5843 c         5530 g         5456 t
ORIGIN      
        1 aatggctgga gctggaaggc gcgaaagatc ttgatgacgc actctctctg ctgaccattc
       61 tttaccacca cgtaccgtcg gtcacatcga tgccggtcta cctagggcaa ctggatgcgt
      121 tgttgcaacc gtatgttaga attctaacac aagacgagat cgatgttcga ataaaacgtt
      181 tctggcgtta cctcgacgga accctgccag acgcctttat gcacgccaat atcggcccgt
      241 ctgattcgcc cattacccgc gcgatcttac gtgcagacgc agagttgaag caggtttcac
      301 cgaacctgac ctttatctac gatcctgaaa tcccccctga tgacctgctg ctggaagtgg
      361 cgaagaacat ctgtgaatgt agcaaaccgc acatcgccaa cggtccggtg catgataaaa
      421 ttttcacaaa agggggctac gggattgtga gctgttacaa ctcactgccg ctggcgggtg
      481 gtggcagcac gctggtacgc cttaacctga aagccattgc cgagcgcagc gaatcgctgg
      541 atgacttctt tacgcgcact ctaccgcact actgccagca gcagatcgcc atcatcgatg
      601 cgcggtgtga attcctctat caacaatcac acttctttga gaatagcttc ctggtgaaag
      661 aagggctgat taaccctgaa cgttttgtgc caatgtttgg catgtatggg ctggcggaag
      721 cggttaactt gctgtgtgag aaagaaggga ttgccgcgcg ctacggtaaa gaagccgccg
      781 caaatgaagt gggttatcgc atcagcgcgc aactggcgga gtttgtcgcc aatacccccg
      841 tgaaatatgg ctggcaaaaa cgcgccatgt tacacgcaca gtcggggatc agttccgata
      901 tcggcaccac gccgggcgcg cgtttgccgt atggcgatga gccagatccg atcatccatc
      961 tgcaaactgt cgccccgcat catgcttatt attattccgg catcagcgac attctgacgc
     1021 tcgacgaaac catcaaacgt aatccgcagg cgctggtaca gctttgcctc ggtgccttta
     1081 aagccggaat gcgtgaattt accgccaatg tcagcggtaa cgatctggtt cgcgttaccg
     1141 gttatatggt gcgtttgtcg gatttagaaa aatatcgcgc cgaaggttca cgcaccaaca
     1201 ccacctggct gggcgaagaa gccacacgca acactcgtat tctggaacgc cagccgcgcg
     1261 tgataagcca tgaacagcag atgcgcttta gtcagtaaga ttatcccctt ctcctgcgtt
     1321 gacgggccag gcagtcgtct ggctctgttt ctgcagggct gcaatctgcg ctgcaaaaac
     1381 tgtcacaatc cgtggacgat gggacgctgc aatgattgcg gagagtgcgt gccacagtgt
     1441 ccgcatcagg cgttgcagat tgttgacggc aaagtgctgt ggaacgctgt ggtctgcgag
     1501 cagtgtgata cctgcctgaa gatgtgtccg caacatgcca cgccaatggc gcaatccatg
     1561 agcgtggacg aagtgcttag ccatgtccgc aaagcggtgc tgtttattga agggataacg
     1621 gtaagcggcg gcgaagccac gacccaactg ccgtttgtag tggcgctgtt tactgctatc
     1681 aaaaacgatc cgcaactgcg ccacctcacc tgcctggtgg acagtaacgg catgttgagc
     1741 gaaaccggct gggaaaaatt actcccggtg tgtgacggcg caatgctcga tctcaaagcg
     1801 tgggggagcg aatgtcatca acacttaacc ggacgcgata atcagcagat taagcgcagc
     1861 atctgtttgc ttgcagagcg cggcaagctg gcggaactgc gtttgctggt aattccagac
     1921 caggtggatt atttgcatca catcgatgag ctggcgacgt ttatcaagag acttggcgat
     1981 gttccggttc gcctgaatgc gtttcatgcc cacggcgtgt atggcgaggc gcaaagctgg
     2041 gcgagcgcca cgccggaaga cgttgagccg ttggctgatg cgttaaaggt gcgcggggtg
     2101 agccggttga tatttccggc gctctatttg tgacatgccg gatacggcct acggttcggc
     2161 acagacttgt aggcatgata agacgcgaca agcgtcgcat caggcattgt gcgccaactg
     2221 ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg taggcatgat
     2281 aagacgcgac aagcgtcgca tcaggcatta tgcgccgact gccggatact gcctacgaag
     2341 ggtaaactac ccactaacgc taaacaacgc atacgtatta ttaagcaaca cttcggcaat
     2401 ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgca cagcctgttc
     2461 cggtcggtta ggctgcccct gaaagccgtt gagcggcata tccggcgcgt cggtttccag
     2521 caacaacgat gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata
     2581 ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct cggcctgttg
     2641 caggctgccg gaaaaaccgt gaaccacgcc agtgcgcgat aaatcgtggc gtttaagatg
     2701 catcgccagt ttgtcgtgtg tgcgccgtga atgcaggatc accggcagat cgtagcgttt
     2761 cgccagtttc agctgttcgt cgagtaacca ctgctgcctc tcaagttgcg gatcgtcgcc
     2821 gaagagatcc agaccgacct cccccactgc caccaccttc gccggacgcc tttccagcgc
     2881 ctgctgtagc tgctcaagag acacatcgct atgtttttcc aacatacctg gatgcaagcc
     2941 caatgcggca tacagcggtt gatatttttc cgctaacgcc tgcacacggg caaaattttc
     3001 cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct
     3061 ggcctcttca tcgccactaa acggcaggaa atcaaaatgg cagtgggtgt cgataaaacg
     3121 gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcgtcaatc
     3181 acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaataacgat cgattccggc
     3241 gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc
     3301 gtggcgagga aataacaacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga
     3361 atccgactgc caagcgcgat actatgtaat ggcttcggcg gataaatttc gaatatccgc
     3421 agcttgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc
     3481 tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg
     3541 aaccactgcg gcgtgtagta catttgtgac ggcacagtgc gaatgacgac caacgtttta
     3601 gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag
     3661 ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga
     3721 atcacatcca gccagttttg tttggttggt aaaaagtaat taggcgcgta gtcatcctga
     3781 cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt
     3841 tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct
     3901 ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg catgatgact
     3961 ttacgcgcgt aaccgggctg attgcagata aacgccgaga ggttctgcgc cccggcagat
     4021 gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca
     4081 gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg
     4141 aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc
     4201 tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa
     4261 aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat
     4321 ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa
     4381 gacaatttta gctgcgcctg cagcggtacc ggccaaacca ccaaacccaa gtgcggcggc
     4441 gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat
     4501 agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg
     4561 tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt acttagtttt
     4621 cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc
     4681 actttgtgcc tgagaatcta cggtaccgga gagctgaacc acgccgtcgg tggtttcaac
     4741 tttcacatga cgggaaggga cgatatcgtc cgccagcagt ttggctttga tttcactggt
     4801 ggtggcggtg tcacccgcgt agcccttcac cgagccttct ttagcgtcgc gaacgtgcag
     4861 tttgtcgctg acagaggtca ccccttcaac gcctttcgcc actttcactg cctcttcggc
     4921 ctgagcctag ctttcaacga aaccgctcag ggtcacgact ttttgctcgg ttttgacaga
     4981 gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt tcgcggtgat
     5041 ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc
     5101 gctttcattg gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg cgacggcaga
     5161 ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt
     5221 attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg
     5281 gagagacttc accctctaca gaagtcaatg gccgccatca caaaagcgat gggtgatgaa
     5341 taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca atcgacggct
     5401 ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg tcattacttt
     5461 gttaaatata gatcacaatt ttgaaaccgc tcgtgatatc agcgaaaaca taagcaaaag
     5521 tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga cagggcgcag
     5581 cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcg
     5641 ggataacgtt cctgcgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta
     5701 tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc
     5761 gcgtccgcac attctaccca gcgggcagtg gcaacgttga ctgattcata tactgcttca
     5821 acgttgtatt cgctcttcag gcgcgatacc accacatcaa actgcagcac accaacagca
     5881 ccaacgatca aatcgttgtt ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa
     5941 agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga
     6001 cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta
     6061 aaggtgtcgc cgatctgaat agtgccgtgg ttgtgcaggc cgaggatatc gcccggatac
     6121 gcttcttcaa cgtgcgaacg gtcgcccgcc ataaaggtca gcgcgtcgga gataaccaca
     6181 tctttcgcag tacgcacctg gcgcagcttc atgccttttt catatttacc ggatactacg
     6241 cgcataaacg ccacgcggtc gcgatgtttc gggtccatat tggcctgaat tttaaatacg
     6301 aagccggtaa atttgtcttc gctcgcctct acggtacggg tatcagtctg acgcggcatc
     6361 ggcgcagggg cccactccac caggccatcc agcatatgat caacgccgaa gttacccagc
     6421 gcagtaccga agaataccgg agtgatttcg cccgcaagga acagttcttt gtcgaactcg
     6481 ttagacgcgc ctttcaccag ttccagttcg tcacgtagct gctgtgccag atcttcacca
     6541 accgcagcgt cgagatccgg gttattcagc cctttaacaa tacggacttc ctgaatcgtg
     6601 tggcctttac cgctctgata aagataggtt tcgtctttat aaaggtggta aacgccttta
     6661 aacagcttgc cgcagccaat cggccaggtg atcggtgcgc agccgatttt cagctcgttc
     6721 tcaacttcat cgagcagttc catcgggtcg cggatatcac ggtcaagttt gttcataaag
     6781 gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt acgatcttca
     6841 acaccttttg cagcgtcgat aaccatcagg cagcagtcca ccgctgtcag ggtacgatag
     6901 gtatcttccg agaagtcttc gtgccccggg gtgtctagca ggttaaccag gcaatcgtga
     6961 tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc catctccatc
     7021 cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccagc ggtctgaatg
     7081 gcctgtccga acagcagcac cttctcggtg atggtagttt taccggcgtc cgggtgagaa
     7141 ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa cgtcataatt
     7201 ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat
     7261 tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc cagttcgtgc
     7321 aacgatgaaa cggtccaggt gggcgcgatg ccttctggtt gctcgagatg gtgcgcattc
     7381 agccagcaag tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc ggcagtgtcg
     7441 ccaaccatca gcacgcgtga acggtcagga ttgcccgcca gttccagcgc ataatcgaaa
     7501 attttcttat tcggtttggc aatgccaact tcttcggaaa tcaccagcaa atcaaagtaa
     7561 tcacgcaggc ccgtccgttc cagacgcact tgctgcaagg cgctaaaacc gttggtgatg
     7621 atgccgattt tagcgttgcc acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc
     7681 gtgcagattt ccgccatcgc attaataaag gcatcgttaa gcaagcctgc ttcaactttc
     7741 agccgttcgg cccagctctc gaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg
     7801 ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa atcttcagcg
     7861 gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta
     7921 aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc accttgtctt
     7981 acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt
     8041 aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca atggcggcag
     8101 cgtttgaagc acggacttcc agccatagtg tcgccacgcc gcgtttttcc agttcgtcga
     8161 tcagatgttt cagcagcacc cttcccaatc cctgacgctg atagtcagga tcgaccacaa
     8221 tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc gccattttgc
     8281 cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca aagttttttt
     8341 cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt aaatcagtcg
     8401 tttcgaggga agaaatcgtg ttcatatgtg caaatctgtt gccataacgc ggcgcgtgcc
     8461 gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat
     8521 agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg
     8581 attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc gcgcagaaca
     8641 tcgctcacta aaggatcggt cagggcggga agatcgtttg ccaccatcac cagacggacg
     8701 tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg cagcgaccac
     8761 tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc gaatcgctcc
     8821 tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata tggctataat
     8881 cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca agtgaagtct
     8941 tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga gacttacagg
     9001 atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag caattccacc
     9061 actggcaggt attaagccgc cagatggggg ataacgcccg tttcagtctg gtcgccacgg
     9121 tggatgacgt cgcagattgc gatacgctga tttactactg gccgaagaac aaaccggaag
     9181 cccagttcca gttgatgaat ttactttctc tgctgccagt ggggacagat atttttgtcg
     9241 ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat tatgcgccgt
     9301 tgaataaagt cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc
     9361 cggtatttga tgccgataaa ttctggggcg aatacagcgt cgatggcctg acggtcaaaa
     9421 cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg ctgctctcga
     9481 cgttaactcc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg ggggtgcttt
     9541 cagttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc
     9601 cagcggtaga agccagccgc gcaacacttg cggccaactg tgttgaaggt gaagtctttg
     9661 ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc aacccgccgt
     9721 tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgc ggcgcggtgc
     9781 gtcatcttaa tagtggcggc gagctgcgaa ttgtagcgaa cgccttcctg ccttatccgg
     9841 acgtgctgga tgaaacattt ggcttccatg aagtgattgc gcaaaccggg cgtttcaagg
     9901 tatatcgcgc cattatgacc cgccaggcga aaaaaggtta agtatcccgc cgcaagcctt
     9961 gctggatgcg gcgtaaacgc cttatccgac ctacaaaacc tgcacgttag cccttcgtag
    10021 gccagataag acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg
    10081 cacgctagtt tcctgatgga catttttcca gcaattacac ctccggcgat aattaactat
    10141 tgacgaaaag ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt
    10201 gatggtatgc gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt agtgtcctta
    10261 cggacgtggg ggttcaagtc ccccccctcg caccaaaaac cacgttgata ttgctcgcac
    10321 tgggcgaagg tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt tcttacggac
    10381 gtgggggttc aagtcccccc cctcgcacca aacgaggcga tatcaaaaaa aagtaagatg
    10441 attgtgcgaa ggtggcggaa ttggtagacg cgctagcttc aggtgttagt gtccttacgg
    10501 acgtgggggt tcaagtcccc cccctcgcac caattatctt tacttccttt cttgtttctt
    10561 ccttgatttt tcatccctga ttcaaattca ttatcatcag catgatggca gcaataagcg
    10621 ccaggcacga tggtagcaaa acgtaatgcc gtaactgttt gtgatacagc atcaccggcg
    10681 tcaatactaa tcccatcaga atgacaatcc gccactgatc cggcgataaa tccgcataca
    10741 tcagccctgc aatcagcaat cccggcagca acactcccca gccactgccc agcgtacgtt
    10801 gcaacatggt ttcatctctt tcattgataa tgataactaa tatcatatga taatttttat
    10861 catttgcaag ccagagaaat cccttgctat cgggtaaacc tatcgctagg attagcaatc
    10921 attatcattt agattactat cccgattatg gcctatcgtt ccgcaccgct ctatgaagat
    10981 atcatctggc gaacgcatct ccagccgcag gatgctgggc ttgcacaagc cgtacgtgcg
    11041 acgatcgcca aacatcgtga acatttgctc gagtttatcc gcctggatga acccgctccg
    11101 cttaacgcca tgacgctggc gcaatggtca tcaccgaatg cgctaagttc tctactcgcg
    11161 gtctattccg atcatatcta tcgcaaccaa ccaacgatga tccgcgagaa caaaccgctg
    11221 atctcactat gggcacaatg gtatatcggc ctgatggtgc caccattaat gctggcgcta
    11281 ctgacgcagg aaaaggcatt agatgtgtcg ccggaacatt tccatgctga gtttcacgaa
    11341 accggacgcg tcgcctgttt ctgggtcgat gtgtgtgaag ataaaaacgc aacaccacat
    11401 tcgccgcagc agcgaatgga aacgttaatc agccaggcgc tggttccggt tgtgcaggca
    11461 ctagaagcga ccggagagat caacggcaaa cttatctgga gtaataccgg ttatttgatc
    11521 aactggtatc tcactgagat gaagcaactg ctcggcgagg cgacggttga atcgctgcgc
    11581 catgccctct tttttgagaa aacgctcacg aacggtgaag ataatccact ctggcgtacc
    11641 gtcgtgctgc gcgatggcct gctggtgcgc cgaacctgct gccagcgtta tcgcttaccg
    11701 gatgtgcagc aatgtggcga ttgtacgctg aaatgaaagc actgccgggg aagtaaaccc
    11761 ggcatcatgc ggattaatag ggatgcaaca cattacttgc ttcgcaatgc cgcatacata
    11821 acagaatatc tgcggcctcc aacacccctg catcagaact gacgcccagt ttcgacatca
    11881 cattaaattt atgtgcacgg atcgttttga tattgcgttt aagctgctcg gcaatttgtg
    11941 tcattgagta gccacgcgac ataaagcgca atatttctct ctccgtcggg cttagcgtcc
    12001 ggctttggtt aatgtaccac tgattgttca gtcggtcggt cgcctgacgt acaccattta
    12061 atgacaaaaa gagttcctga tgaaaaatct ccagcgttga cgctttactt aatacaccgt
    12121 ccagcggtga tggcgacaat gaaccaatca gccgagcttc aatatcatca tccgcaataa
    12181 ctaaacgccg ggtacgcgga aacttaatcg ccagttcagt caggcaagat aatccctctc
    12241 tgcgctcact tctcatggca gaaaaagaaa aatgaccgcc gcaaatgaga tccgcgacat
    12301 ggcatcctga aatgactcct gactggtata ggtatggagc ttataagggt tacccgcaaa
    12361 ctttctgaat aaactctcaa taccgatact gctcatgacg catttttcta tgagtgcgac
    12421 atttctcttc ttaattcggc tgtgttccat tctgcggcat ctccataaac agaaggcaag
    12481 ttaagttctc ttgcaccctg cgcgctattt atccatgcat acatttcagc gttgctgtga
    12541 attgccagtc gacgcatcgc actgtttttt tgggcgctga tagttttatt acttttctta
    12601 agcaacgaaa ctatctggtt gatgccccat cctttaccta atagccgcag taccttgcgc
    12661 tctgaaagag tgagaatgac ggagcgatag ctaaaatcat gagtttcatt cattgccgga
    12721 gaggtcagca tggcgctgat acgctctgcg tgcgtattgc cggaacgtac ggtcttgacc
    12781 agattctcaa tgggttcaac atcagacaat aacgtcgccg tagggcacat gagcaattct
    12841 actgcctggg aataccagga gcgtgacacc atgaaaaccc agtgaatttc ccgatattgt
    12901 gagattaaag aataataatg ttcacaaaca gaacgtggat cttcactttc accggctaaa
    12961 tcagcaatga ctaatccgga acgacgtaat tgtaataagg taaggtcctc cgcagaggcg
    13021 ctggatatta tttcatattc agggaagtga gtcctcatga cctcttttaa ccctgcttgc
    13081 ataacaggta ttttactgat aacaattcca tttttgcagc atcctggcaa catatttcct
    13141 ccacatccac tatctaaaaa tcatgatggt ttattcagga tattcattcc ttaaatgttt
    13201 ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc atatcagcat
    13261 actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa atgataacct
    13321 agtgaaatag cctggagaca tttaaaatat ctacattaag ttgttaggaa tacttaatga
    13381 aaaacagaaa tcacattcat ttaatttata attaaatgat atcccttccc gcagaatgaa
    13441 aagtcacaca accaatattt attcaatgaa atctgatggt tttaacttgt taacattaaa
    13501 aaacgtgccc ggttatctat tatcctgatt cgtaaaggcc aaacaataaa ttcattatag
    13561 tgataatatt catgtgatag tttgtttgcg gcgagagata attcgctttt tatcaccgag
    13621 cttgtcccga taaccaggat taatcagcgc aattttgatc ttaacctacc gcaaaacaac
    13681 cgtgcttcat gatacaaatg gattctcatt atctgaacaa tacgcagcac gtctatgaca
    13741 aagggcgagt tatgcaaact gagcaacagc gagccgtaac acggctttgt atccagtgtg
    13801 gattatttct tttgcaacat ggtgcggaaa gcgcgttggt tgatgagctt tcctcacgac
    13861 tgggtcgggc gttggggatg gacagcgtcg aaagttctat ctcttcgaac gccatagtgc
    13921 tgacaaccat taaagatggg caatgcctga catcgacacg taaaaatcac gatcgcggca
    13981 ttaatatgca tgtggtgact gaagtccagc acattgtgat tctggcggag caccatctgc
    14041 tggattacaa aggcgtagag aaacgattta gccaaattca gccattacgt tacccaagat
    14101 ggctggtagc cttaatggtt ggcctttctt gcgcctgttt ctgcaaactc aataaaggtg
    14161 gctgggatgg tgccatcatc accttctttg ccagtacggc cgcgatgtat atccgccagc
    14221 tgctggcaca acatcatctt catccgcaga tcaacttttg cctcaccgct ttcgccgcca
    14281 ccaccatttc cggattgctt ttgcaacttc ccactttcag caatacaccc actatcgcga
    14341 tggccgccag cgttctgctg ctggtgccgg gatttccgtt gattaatgcc gtcgccgata
    14401 tgtttaaagg ccacatcaat accggactgg cacgctgggc gatcgccagt ctgctgacac
    14461 tggctacctg cgtcggcgtg gtgatggcac tgacgatttg ggggctacgc ggatgggtgt
    14521 gatcgagttt ctgtttgcat tggcgcagga tatgatcctc gccgccattc cagcggtcgg
    14581 ctttgcgatg gtgttcaacg ttcccgttcg ggcattacgc tggtgtgcgc tgcttggcgc
    14641 gattggtcat ggttcacgaa tgatcttaat gaccagaggg ctgaatattg agtggtcaac
    14701 ctttatggcc tccatgctgg tcggcaccat tggtattcaa tggtcgcgct ggtatctggc
    14761 gcatccgaaa gtgtttaccg tggcggctgt tatccctatg ttcccgggca tatcggctta
    14821 taccgcaatg atttcggcgg taaaaatcag ccagttaggt tacagcgaac cgttgatgat
    14881 taccctgtta accaactttc ttacagcttc atcgattgtt ggtgcgttat ccatcggtct
    14941 ttccattcct ggattatggt tgtaccgcaa gcgccctcgc gtataaaatt gcctcgctta
    15001 aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa gttatctccc
    15061 ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt gaccccggac
    15121 gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa agctgaaggc
    15181 ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc
    15241 ctggctgaac tgctggcgct ggaagaaaag ctggcgcgtc cgggaagcga tgtcgctctg
    15301 gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat ggggaaattc
    15361 gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc gctatggggt
    15421 gtggcactaa gagagccggt gaccaccgaa gaactggcct cattcattgc ctactggcag
    15481 gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa
    15541 atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt cagcgaacct
    15601 gacagtcaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc gacctgatgc
    15661 aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg ggtgaagaac
    15721 tactggcctg gcagaaagaa caaggagcga tccgctccgc cgctctcgaa cgtgaaaatc
    15781 gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcggccactg catcagaact
    15841 gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc
    15901 gccagtacgt cgaagagttt gacggcaaca tcgccagctt catcttttct ggtaagccag
    15961 gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat
    16021 ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc ttcaggaata
    16081 gcggtaccag tgaagaacaa ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg
    16141 atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg
    16201 atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat atggaagaga
    16261 tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac agtttgtggg
    16321 tgatcttcaa ctgggatagc taccgtagcc gggtaacagg taaagagtat taatccccgt
    16381 ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa actgttaaac
    16441 atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg catgctgcgt
    16501 cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac agcacgacac
    16561 aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcggg aaccaggcct
    16621 taagcgcaga taacatgaac aacgccgcag gcattctgca atactgcgcg aagcaaaagc
    16681 tggcgtcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag ctgggcctga
    16741 acagtgaaga gcaaaaagaa gacaccaact atctggatgg cattcagggt ttgctgaaaa
    16801 ccaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg gcagaaaagg
    16861 tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt tcctgaaccg
    16921 cgaaacgccg cgttttagct gttacccggc tgacgcggct ctctcctgcc cttttccctg
    16981 cttctggacg gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa agtctgttta
    17041 taaactaatt cctaacaaca tcgcattttt ttgacactag aattgcagca atatggcgac
    17101 gccattacat tgttcttctc aaaaagctta aacgttggct aaacggcccg gtttagtgag
    17161 gatcgcccgt tgtcagaact actctctttc gccctttttc tcgcctctgt gctgatttac
    17221 gcatggaaag cgggacgtaa cacctggtgg tttgcagcca cattaacggt gctggggcta
    17281 tttgtcgttt taaatatcac cctgtttgcc agcgactatt ttactggcga tggtattaac
    17341 gacgcggttc tctatacctt aaccaacagc ctgaccggtg ctggcgtcag caaatacatc
    17401 ctgccgggta tcggcattgt gctggggctg acagcggtgt tcggtgcgct gggctggatc
    17461 ctgcgccatc gtcgccatca tccgcaccat tttggttaca gcctgctggc gctcttactg
    17521 gcgctgggtt cggtggacgc cagcccggca tttcgtcaga taacggaact ggtgaaatcc
    17581 cagtcacgcg atggcgaccc ggactttgcg gcttattata aagagccgtc gaaaactatc
    17641 cctgacccga aactcaacct ggtttatatc tacggcgaaa gtctcgagcg gacctatttt
    17701 gataacgaag ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg
    17761 gatttcagcc acacgcagca gctgccagga acggattaca cgattgcggg catggtggct
    17821 tctcagtgcg gcataccgct gtttgccccc tttgaaggca acgcctccgc ctctgtctcc
    17881 agcttcttcc cgcagaacat ctgtctgggc gatatcctga aaaactcggg ttatcagaac
    17941 tatttcgtgc agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct gaagtcgcat
    18001 ggcttcgacc acttatacgg ctcagaagag ctgaaaagcg tggtggccga cccgcactat
    18061 cgcaacgact ggggattcta cgacgatacc gttctcgatg aagcgtggaa aaagtttgaa
    18121 gagctttccc gctcaggtca gcgattctca ctgtttaccc tgacagtcga tacccatcac
    18181 ccggatggtt ttatctcgcg tacctgtaac cgcaaaaaat atgattttga cggtaagccg
    18241 aaccagtcat tcagcgcggt aagttgcagc caggagaata tcgcggcgtt tatcaacaaa
    18301 atcaaagcgt caccgtggtt taaagatacc gtcatcgtcg tctcttctga ccatttagcg
    18361 atgaacaaca cggcgtggaa atacctcaat aagcaggatc gcaataacct gttttttgtc
    18421 attcgtggcg acaagccgca gcaagagacg ctggcagtga agcgtaacac gatggataac
    18481 ggcgcgacgg tgctggacat tctcggtggc gataactatc tcggacttgg tcgtagcagt
    18541 ttatccgggc agtcgatgtc ggaaatcttc ctcaatatca aagagaaaac attggcgtgg
    18601 aagccggata tcatccgcct gtggaaattc cctaaagaga tgaaagagtt caccatcgac
    18661 cagcagaaaa acatgattgc cttctcgggt agccatttcc gtttgccgct gctgttgcgg
    18721 gtttcagaca aacgcgtgga accgctgccg gaaagcgaat actcagcacc gctgcgtttc
    18781 cagctggccg atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg ctataagatg
    18841 gcacaactct gggctccgga actggcactc tccaccgact ggtgtgtctc gcaagggcag
    18901 cttggcggtc agcaaattgt tcaacatgtt gacaaaacaa catggcaggg caaaacggca
    18961 tttaaagata cggtcatcga catggcgcgt tataaaggca atgtcgatac gctgaaaatt
    19021 gttgataacg atattcgcta caaagccgac agtttcatct tcaacgtcgc cggtgcgcca
    19081 gaagaggtga aacagtttag tgggatttcc cgtccagagt cgtggggccg ctggtccaac
    19141 gcgcagctgg gcgatgaagt aaaaatcgag tacaagcatc cgctgccaaa gaaatttgac
    19201 ctggtgatta ccgccaaagc atacggcaat aacgccagcc gtcctattcc ggtacgcgta
    19261 ggcaatgaag aacaaaccct tgtgctgggc aatgaagtga ccaccaccac actgcatttc
    19321 gataacccaa ccgatgccga cacactggta attgtgccgc cggaacctgt ctcaaccaac
    19381 gaagggaata tcctcggaca ctcgccacgt aagctcggga tcggcatggt ggaaattaaa
    19441 gtggtggaac gtgaaggata attcctgact gatgtgtagg tcgcataagg tgcgacctac
    19501 acaccttctg cggcctacaa aacatcattg agaaacagaa aaaaacagcg cccaacactt
    19561 ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa ttacaccctt
    19621 aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg tggcgaacgg
    19681 atagcgatgg gttaacatca tgtcagcagt gatttttcct tccgccatca gtcgaccgac
    19741 tttagcaaaa tcttccggcg tggcgttgcg gctacccatc atcgtcgttt cttttttatg
    19801 gaattccgga tcggagaact gcaactcacc tttaaacagg ccgacaaata ccaccgtgcc
    19861 gccgtgacga atcaaattca cggtgttatt catcgcatgt tgattacctg tcgcgtcgat
    19921 cactttctgc gccagcgaac caccaaactg cgcccgtagc tgcgcgtcaa aatcttcggc
    19981 tgacgggtcc agtaaaggta attccagacg cgttgccaca tgttcacggc gcgccggact
    20041 ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg cgcccagacc
    20101 gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg cgcgacgcac
    20161 cgcatgagcg ctaatagcga aaggttcaat caatgctgcc gcctgcgggt caataccgtc
    20221 tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc catcctgatg
    20281 cacgccaatg actgaaattt tttcacagca attggtacgc ccgcttttac acgccgggca
    20341 ttgctgacag gcaacataag ggatcacagc aacttgcaga ccatttttaa gatcagcaat
    20401 atttttaccc agcctaacaa tctccccaca tatttcatgg cctaaaacac gtggataact
    20461 aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc cgacagactt
    20521 aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt tccagactaa
    20581 ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc tctccatttt
    20641 ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa tctgttttgt
    20701 gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa ggaggaaaat
    20761 atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga tgatatggcg
    20821 cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat gtacaacatc
    20881 agccgcacca cagtgcgtca tattctcagc catttacgcg aatgcggcgt cttgacgcag
    20941 gttggaaacg actatgttat tgcgcgtaaa cctgaccacg atgacggttt cgcctgtact
    21001 accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac gctgataaac
    21061 cagcgccagt tacgcccagg ggaaactttt tctgaactgc aactggctcg ggccgcgggt
    21121 gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa tcttattcaa
    21181 agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc cgagcagctg
    21241 tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa cctgcctgat
    21301 catgatcccc gctggctgca ggcgaaaacc atgctcgaac gtcatcggct actgcgtgac
    21361 aacatcggca atagctttcg catgttctcg caacttgaca gagattttca ctccttactg
    21421 ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc agtcatcttt
    21481 cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat tgccgtcgat
    21541 gagcatatga ccattctcag cgctctgatt tgccgcagtg acctcgatgc cacccttgcg
    21601 ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa tgagaacacg
    21661 cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc acggttttat
    21721 caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc gactaaaaat
    21781 gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt caaccttgcg
    21841 taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg tggaaaaaga
    21901 aaatatcacc atcgatccgc gttcttcatt tactccatct tcgtcggcag atattcccgt
    21961 gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa ccaccgccat
    22021 gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc tgtcggtagc
    22081 aaatttaacg attcgtgaag aattgggatt aagtgccact gaaatcggcg ctttgctctc
    22141 cgtgttttca ctcgcttacg ggattgcgca acttccttgc ggcccactat tggatcgtaa
    22201 aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc aggcaatgtc
    22261 tggcatggtg cacaacttta cgcagttcgt gttggtgcgt atcggtatgg ggattggtga
    22321 agcgccgatg aacccatgcg gtgtaaaagt
//
LOCUS       DAJTOR010000056        22008 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTOR010000056 DAJTOR010000000
VERSION     DAJTOR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.056"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..1090)
                     /gene="actP"
                     /locus_tag="OO249_001892"
     CDS             complement(<1..1090)
                     /gene="actP"
                     /locus_tag="OO249_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5636209.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAV"
     gene            complement(1087..1401)
                     /locus_tag="OO249_001893"
     CDS             complement(1087..1401)
                     /locus_tag="OO249_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5636210.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(1533..3492)
                     /gene="acs"
                     /locus_tag="OO249_001894"
                     /pseudo
     CDS             complement(1533..3492)
                     /gene="acs"
                     /locus_tag="OO249_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            3885..5321
                     /gene="nrfA"
                     /locus_tag="OO249_001895"
     CDS             3885..5321
                     /gene="nrfA"
                     /locus_tag="OO249_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5636211.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5366..5932
                     /gene="nrfB"
                     /locus_tag="OO249_001896"
     CDS             5366..5932
                     /gene="nrfB"
                     /locus_tag="OO249_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5636212.1"
                     /translation="MSVLRSLLTARVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            5929..6600
                     /gene="nrfC"
                     /locus_tag="OO249_001897"
     CDS             5929..6600
                     /gene="nrfC"
                     /locus_tag="OO249_001897"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5636213.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            6597..7553
                     /gene="nrfD"
                     /locus_tag="OO249_001898"
     CDS             6597..7553
                     /gene="nrfD"
                     /locus_tag="OO249_001898"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5636214.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLAQQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            7573..9291
                     /locus_tag="OO249_001899"
     CDS             7573..9291
                     /locus_tag="OO249_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5636215.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9284..9667
                     /gene="nrfF"
                     /locus_tag="OO249_001900"
     CDS             9284..9667
                     /gene="nrfF"
                     /locus_tag="OO249_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5636216.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            9664..10260
                     /gene="nrfG"
                     /locus_tag="OO249_001901"
     CDS             9664..10260
                     /gene="nrfG"
                     /locus_tag="OO249_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5636217.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYVALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            10601..11914
                     /gene="gltP"
                     /locus_tag="OO249_001902"
     CDS             10601..11914
                     /gene="gltP"
                     /locus_tag="OO249_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5636218.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12105..12793)
                     /locus_tag="OO249_001903"
                     /pseudo
     CDS             complement(12105..12793)
                     /locus_tag="OO249_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(12887..15034)
                     /gene="fdhF"
                     /locus_tag="OO249_001904"
     CDS             complement(12887..15034)
                     /gene="fdhF"
                     /locus_tag="OO249_001904"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(14615..14617),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5636219.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIMANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15232..16698)
                     /gene="mdtP"
                     /locus_tag="OO249_001905"
     CDS             complement(15232..16698)
                     /gene="mdtP"
                     /locus_tag="OO249_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5636220.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGIAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(16695..18746)
                     /gene="mdtO"
                     /locus_tag="OO249_001906"
     CDS             complement(16695..18746)
                     /gene="mdtO"
                     /locus_tag="OO249_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5636221.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSCSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(18746..19777)
                     /gene="mdtN"
                     /locus_tag="OO249_001907"
     CDS             complement(18746..19777)
                     /gene="mdtN"
                     /locus_tag="OO249_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5636222.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDVLVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPNDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(19796..20071)
                     /locus_tag="OO249_001908"
     CDS             complement(19796..20071)
                     /locus_tag="OO249_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636223.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(20280..>22008)
                     /gene="yjcS"
                     /locus_tag="OO249_001909"
     CDS             complement(20280..>22008)
                     /gene="yjcS"
                     /locus_tag="OO249_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR5636224.1"
                     /translation="GEDYKFDINAPAPETVNPSLWRQSQLNGISGLFKVTDRMYQVRG
                     QDISNITFIEGDTGIIVIDPLVTPPSAKAALDLYFQNRPQKPIVAVIYTHSHTDHYGG
                     VKGIISEADVKSGKVQVIAPAGFMDEAISENVLAGNIMSHRALYSYGLLLAHNPQGNI
                     GNGLGVTLASGYPSIIAPNKTITKTGEKMIIDGLEFDFLMTPGSEAPAEMHFYIPALK
                     ALCTAENATHTLHNFYTLRGAKTRDTSKWTEYLNETLDMWGNDAEVLFMPHTWPVRGN
                     KHINDYIGKYRDTIKYIHDQTLHLANQGYTMNEIGDMIKLPPALANNWASRGYYGSVS
                     HNARAVYNFYLGYYDGNPANLHPYGQVEMGKRYVQALGGSARVISLAQEANKQGDYRW
                     SAELLKQVIAANPGDQVAKNLQANNFEQLGYQAESATWRGFYLTGAKELREGVHKFSH
                     GTTGSPDTIRGMSVEMLFDFMSVRLDSAKAAGKNISLNFNMSNGDNLNLTLNDSVLNY
                     RKTLQPQADASFYISREDLHAVLTGQAKMADLVKAKKAKIIGNGAKLEEIIACLDNFD
                     LWVNIVTPN"
BASE COUNT         4916 a         5793 c         6019 g         5280 t
ORIGIN      
        1 caaccgctga aataaaaccg aggaacaggt tgccgcccac tgcattcgcc aggtgaacgg
       61 ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc ggattcgcac
      121 caaccagcat gatcgcgccg aagccgataa taaaggtcag aatatagaag tagcccataa
      181 acccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact gtaaagaagc
      241 gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagaccc agagagagcg
      301 cggagatcgg atctttcacc agcccgcccg gcttcatgat gtcgacacct ttcgggtgta
      361 ccgccatcgc ttcgctgaac agattattga agctaaagcc gacgtgtttc atcaccataa
      421 aggccataaa gctggcaccg aacagcaaca gcacggcttt aataatttgc acccaggtgg
      481 tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc agcaccaccg
      541 caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct accatctggg
      601 cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata cgaatcggcc
      661 cttgtttcag gcggtaagag gctacatcgg caaaggtgta gcgtcccagg ttacgcagac
      721 gttcggcaat caggaacaaa atgatcggcc agcccaccag gaagcccagc gagtaaatta
      781 agccgtcata gccggaggta aacaccagcg cggagatccc caaaaatgag gcggcggaca
      841 tatagtcacc ggcaatcgcc agcccgttct ggaagcctgt gatattgccg cctgccgtgt
      901 agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg agcgtaaaca
      961 cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc tctacggccc
     1021 cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc gccgtcagaa
     1081 ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt cgaattcgcc
     1141 gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca ctccaacacc
     1201 aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca gccagccggg
     1261 cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa tcgacaggat
     1321 ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt cttctatccg
     1381 ctgataaata gtgccattca tcacagattc tccagaggta atgtagggat tgttttaatt
     1441 cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg taggccggat
     1501 aaggcgttta cgccgcatcc ggcaattgtg ggttacgatg gcatcgcgat agcctgcttc
     1561 tcttcaagca gcttctcgac tacgccagga tcggcaagcg tcgaggtatc gcccaggttg
     1621 ctggtatcgc ccgccgcaat tttgcgcaga atacggcgca taattttgcc ggagcgggtt
     1681 ttaggcaggg agtcggtcca gtgcagcacg tctggcgtcg ccagcgggcc aatctcttta
     1741 cgcacccagt tgcggacttc tgcgtacagt tctggtgacg gttcctcccc gtgattaagc
     1801 gtgacgtagg cgtagatcgc ctgaccttta atattgtgcg gaatacctac tacggcggct
     1861 tcggcaatct tcggatgcgc caccagcgcc gactcaatct ctgccgtccc cagacggtga
     1921 ccggagacgt tcagcacgtc gtccacatgc ccggtgatcc agtaatagcc atcttcatcg
     1981 cgacgcgcgc cgtcgccgct gaaatacata tttttgaagg tggagaagta ggtctgttca
     2041 aaacgttcgt gatcgccaaa cagcgtacgc gcctgacccg gccaggagtc ggtgatcacc
     2101 aggctgcctt cggtagcgcc ttccagcggg ttaccttcgt tatcgaccag cgccggttgc
     2161 acgccgaaga acggacgtgt tgccgaaccg gctttcagcc cggtagcgcc aggcagtggc
     2221 gtgatcatga aaccgccagt ttcggtctgc caccaggtat cgaccaccgg acatttctcg
     2281 ttgccgattt ttttccagta ccactcccac gcttccgggt taattggctc gcccacggaa
     2341 ccgagaatgc gcagcgacga acggtcggta ccctcgatcg ctttatcgcc ttccgccatc
     2401 agcgcgcgga tcgccgtggg tgcggtatag agaatattga cctgatgctt gtccaccacc
     2461 tgcgccatac gggcaggcgt cggccagttg ggtacgcctt caaacatcag cgtggtcgca
     2521 ccgcaggcca gcgggccgta cagcaagtaa ctgtgtccgg tcacccagcc cacatcggcg
     2581 gtgcaccagt agatatcacc cggatgataa tcaaagacat atttaaaggt cagcgccgcg
     2641 tacaccagat aaccgccggt agtatgcagc acaccttttg gcttaccggt agaaccggag
     2701 gtgtagagaa taaacagcgg atcttcggcg ttcatctctt ccgcctggtg ctgatcgctc
     2761 gcttgctcaa ccaggtcgtg ccaccacagg tcgcgccctt cctgccagtc aattttcccg
     2821 ccagtacgct tcagtaccac cacatgctct acgctggtga cgttcgggtt tttcagcgcg
     2881 tcatcaacgt ttttcttcag cggaatactg ccgcccggca cgcacacctt cgtcggaagt
     2941 gatcaccagt cgtgagttgg aatcaataat gcgcccggca acggcttccg gcgagaagcc
     3001 gccgaaaatc accgaatgca ccgcgccaat gcgggcgcag gccagcatcg caaccgcggc
     3061 ttccggcacc atcggcatat aaatcgccac cacatcacct tttttaatgc ccagctcgag
     3121 cagggtattg gcgaagcggc agacgtcgcg gtgcagctct ttatagctga tatgtttgct
     3181 ctggctggcg tcgtcgcctt cccagatgat ggcggtacga tcgccgtttt cttgcagatg
     3241 gcggtcaagg cagtttgccg ccagattcag cgtgccgtcc tcgtaccatt taatggacac
     3301 attaccgggg gcaaaggagg tgtttttcac cttctggtaa ggtttgatcc agtcaagaat
     3361 ttttccctgt tcgccccaga aggtatcagg tacgttaata gattgttgat acattgcctc
     3421 gtactgctga gggtttatca ggcaacggtc tgcgatgttg gcaggaatgg tgtgtttgtg
     3481 aatttggctc atgcttttgt tctccttgta gaatgttaat aatatgtgac ataagcgtta
     3541 aatgtagggg tattggcagt tttgtttagt atttgggcga cagatcacgc aaaagtagaa
     3601 ttgtgcaaat aaacggcagg gtaatttttg aaggtcagca acaaaagttg attaattctt
     3661 tgaggaacat gcagttatgc atgctgttga aaaagaggaa gatactgact aactctaaag
     3721 tggtatttta catgcactta caattgatta aagacaacat tcacagtgtg gttatttgtt
     3781 acacataggg gcgagcaatg tcatgacagt gtaggtgcgg ttactgtcgt gaaaaacaat
     3841 aaaaaccgcc attgcaacaa tggcgcaatt cggatgaagc ccctatgaca aggataaaaa
     3901 taaacgcacg ccgtatcttc agcttattga ttcctttttt ctttttcact tctgttcacg
     3961 ctgaacaaac ggcagctccc gcaaaacctg taactgtgga agcgaagaat gaaacctttg
     4021 ccccgcagca tcccgatcaa tatctctcct ggaaagccac ctcggaacag tcagagcgtg
     4081 ttgacgccct ggcggaagat ccacggctgg tgatcctgtg ggcggggtat cccttctcgc
     4141 gcgattacaa caagccgcgt ggacatgcct ttgctgtgac cgatgtgcgt gaaaccctgc
     4201 gtaccggtgc gccgaaaaat gctgaagatg gtccgctacc gatggcgtgc tggagttgta
     4261 aaagcccgga tgtggcgcgt ctgatccaga aagacggcga agatggctac ttccacggta
     4321 agtgggcgcg cggcggcccg gaaatcgtca acaacttagg ttgtgccgac tgccataaca
     4381 ccgcctcacc agagttcgcc aaaggcaaac cggagttaac cctttcccgt ccgtatgcgg
     4441 ctcgcgcgat ggaagccatt ggtaaacctt ttgagaaagc cggacgtttc gaccagcaat
     4501 cgatggtttg cggtcagtgc catgtggagt attacttcga cggcaaaaac aaagcggtta
     4561 aattcccgtg ggatgacggc atgaaagtcg aaaatatgga gcagtattac gacaaaattg
     4621 ccttctctga ctggactaac tccctgtcga aaacgccaat gctgaaagcg cagcacccgg
     4681 aatatgaaac ctggacagcg ggcattcacg gtaaaaacaa cgtgacctgt atcgactgcc
     4741 atatgccaaa agtgcagaac gccgaaggca aactctacac cgaccataaa attggtaatc
     4801 cgtttgataa cttcgcccag acttgtgcga actgccatac ccaggacaaa gctgccttgc
     4861 aaaaagtggt cgcggaacgt aagcagtcga ttaacgacct gaaaatcaag gttgaagatc
     4921 aactggttca cgctcacttc gaagcgaaag cagcgctgga tgcaggcgcg acggaagctg
     4981 aaatgaagcc aattcaggac gatatccgtc atgcccagtg gcgctgggat ctggcgatcg
     5041 cttcccacgg cattcatatg cacgcaccgg aagaaggttt acggatgctc ggtacggcga
     5101 tggataaagc ggcggatgca cgcaccaaac tggcgcgcct gctggcgacc aaaggcatca
     5161 cccatgaaat ccagatcccg gatatctcaa ccaaagagaa agcccagcag gccattggcc
     5221 tgaacatgga acaaatcaag gccgagaagc aggacttcat caaaacggtg atcccgcagt
     5281 gggaagaaca ggcacgtaaa aacggtctgt taagccaata accccgttcc gcctcgcaag
     5341 gggcggaaaa cacaatggag tgaatatgag cgtattacgt tcgttattaa ctgctagggt
     5401 gctggcgtcg ggcctgttgt ggagcctgaa cggaattacc gctacccctg cggcgcaggc
     5461 atctggtgat cgctacgaag ttacccagca gcgtaacccg gatgccgcct gtctggactg
     5521 tcataaacca gataccgaag gtatgcatgg caaacatgct tccgttatca acccgaataa
     5581 caaattgccg gtcacctgca ccaactgtca cggccagcca tcaccgcaac accgcgaagg
     5641 ggtgaaagat gtgatgcgct ttaacgagcc gatgtacaag gtcggagagc agaacagcgt
     5701 ctgtatgtcc tgtcatctgc cggaacagtt gcaaaaagcg ttctggccgc acgatgtcca
     5761 cgtaaccaaa gtggcgtgcg ccagctgcca ttccctgcat ccacagcaag atacgatgca
     5821 gacgttaagt gacaaaggac ggattaagat ttgcgtcgat tgccacagcg atcagcgcac
     5881 caatccgaac tttaacccgg cgtctgttcc gttgcttaag gagcagccat gacctggtct
     5941 cgtcgccagt ttctcaccgg cgtcggcgtg ctggcagccg tcagcggcac cgcagggcgc
     6001 gtggtggcga aaacgttgaa tatcaatggg gtgcgttacg gcatggtgca tgacgagtcg
     6061 ttatgcatcg gctgtaccgc ctgtatggat gcttgtcgag aagtgaacaa ggtgccggaa
     6121 ggcgtctcgc gcctgacgat tattcgtagc gagccgcagg gcgaatttcc tgatgtgaaa
     6181 tatcgcttct tccgtaagtc ttgccagcac tgcgatcatg cgccgtgcgt tgacgtctgc
     6241 ccgaccggtg cgtcttttcg tgatgccgct agcgggattg tcgatgttaa tcctgatctc
     6301 tgcgtcggtt gtcagtactg catcgccgcc tgtccgtacc gcgtgcgctt tatccatccg
     6361 gtcacgaaga cggcggacaa atgcgatttc tgccgtaaaa ccaatttgca ggccggtaag
     6421 ctgcccgcgt gcgttgaggc ttgcccgacg aaggcgctga cgtttggcaa tctggacgat
     6481 cctaacagtg agatttcgca actgctgcgc cagaagccta cttaccgcta caagctggcg
     6541 ctgggaacca aaccgaagct gtaccgcgta ccgtttaaat acggggaggt gagccaatga
     6601 cgcagacttc cgcatttcat tttgaatcgc tggtgtggga ctggccgatt gccatctacc
     6661 tgtttttgat tggtatttct gccggtctgg tgacgctggc cgtgctgtta cgtcgcttct
     6721 acccgcaggc gggcggtgca gacagtacgt tgctgcgcac cacgctgatt gtcgggccgg
     6781 gcgcggtgat cctcggtctg ttgatcctcg tcttccacct gacaagaccg tggaccttct
     6841 ggaagctgat gttccactac agttttacct cggtgatgtc gatgggggtg atgctgtttc
     6901 agctttacat ggtggtgctg gtgctgtggc tggcgaaaat ctttgaacat gatttgcttg
     6961 cccagcaaca acgctggttg ccgaagctgg ggatcgtgca aaaggttctg agcctgctga
     7021 cgcccgttca tcgcggactg gaaacgctga tgctggtgtt ggcggtgctg ctgggggctt
     7081 ataccggctt tctgctgtcg gcgctgaaat cgtatccgtt cctcaataac ccgatcctgc
     7141 cggtgctgtt cctcttctcc ggcatctcgt ccggtgcggc ggtggcgctg atcgccatgg
     7201 cgatacgcca acgcagtaac ccgcattcca cggaagcgca ctttgttcac cggatggaaa
     7261 tccccgtggt atggggtgaa atcttcctgc tggtggcgtt ttttgtcggt ctggcgctgg
     7321 gcgatgacgg taaagttcgt gcgctggtgg cggcattagg tggcggtttc tggacgtggt
     7381 ggttctggct tggtgtcgcc gggctggggc tgattgtgcc aatgttgctc aaatcgtggg
     7441 tcaatcgcag ttccggcatt cctgccgtgc tggcggcgtg tggggccagt ctggtcggcg
     7501 tgttgatgct gcgctttttc attctctacg ccggacagtt gacggtggcg taagccagaa
     7561 aagaggtggt ttctggacgt attccttcct gaagtcggtt ttctggcgtt gttgttaagt
     7621 ctcggggtca acgtgttgac cccgttgacg gcctttgcgg gagtgcggtt gcgctggcct
     7681 gccatgatgc gactcacttg catcggcatt ctggcgcagt gcgcgctcct gctgctcgcc
     7741 tttggcgtac tgacgtattg ttttctcatc agcgatttct cggtcattta tgtcgcccaa
     7801 catagctaca gcctgctgtc gtgggaactc aaactggcgg cggtgtgggg cggtcatgaa
     7861 ggttcgctgc tgctttgggt gctgctgctt tccgcctgga gcgcgctgtt tgcctggcat
     7921 tatcggcagc aaaccgatcc gctatttccg ctgacgctag ccgttttatc tctcatgctc
     7981 gccgcactgc tactgtttgt ggtgctgtgg tccgatccct tcgtgcggat atttccacca
     8041 gcaatcgaag gccgcgatct caatccgatg ctgcaacatc ccggtcttat ctttcatcca
     8101 ccgctgcttt accttggcta tggcggtttg atggtagcgg cgagcgtggc gctggcgagt
     8161 ctactgcgcg gcgagtttga tgcggccagc gcccgaattt gctggcgctg ggcgttacct
     8221 ggctggagtg cattaacggc ggggatcatc ctcggttcct ggtgggccta ctgcgaactc
     8281 ggctggggcg gctggtggtt ctgggatccg gtggaaaatg cctctttatt accctggctt
     8341 tctgccactg cgctgctgca cagtttatcc ctgacacgcc agcgggggat ttttcgccac
     8401 tggtcgctgt tactggcgat agttactctg atgctgtcgc tgctgggcac cttaattgtc
     8461 cgttctggca ttctggtttc ggttcatgcg ttcgcgctgg ataacgtccg cgccgtgccg
     8521 ttgttcagcc tgtttgcact gattagcctt gcgtctctgg ctctgtatgg ctggcgagcg
     8581 cgggacggtg gcccggcggt gcgtttttcg gggttatcgc gggaaatgtt aatcctcgct
     8641 acgctgttgc tgttttgcgc agtgctactg atcgtgctgg tgggaacgct ttatccgatg
     8701 atttacggtc tgctgggctg gggacgcctc tccgttggcg cgccgtattt taaccgcgcg
     8761 acgttaccgt ttggtctgtt gatgctggtg gtgattgtgc tggcgacgtt tgtctctggc
     8821 aaacgcgtgc agcttccggc gctggtagct catgcgggcg tgctgttatt tgccgctggg
     8881 atcgtggttt ccagcgtcag ccgtcaggag atcagcctga atttacagcc gggtcagcag
     8941 gtgacgctgg caggatacac cttccgtttt gagcgcctcg atctgcaagc caaaggcaat
     9001 tacaccagcg aaaaagcgat agtggcactg tttgaccatc agcaacgcat tggtgaactg
     9061 atgccggagc ggcgttttta cgaagcacgt cgtcagcaaa tgatggaacc gtcaattcgc
     9121 tggaacggca tccatgactg gtatgtggtc atgggtgaaa aaaccggagc ggatcgttac
     9181 gcttttcgct tgtatgtaca aagcggtgtg cgctggatct gggggggagg attgttgatg
     9241 attgcgggcg cattgttaag cggatggcgg gggaggaagc gcgatgaata aaggacttct
     9301 cacgctactg ctgttattta cctgttttgc gcgcgctcag gtcgtagaca cctggcaatt
     9361 cgccaatccg caacaacagc aacaggcgtt aaatattgcc agccagttac gttgtccgca
     9421 gtgccagaat caaaacttac tggaatccaa cgcgccggtg gcagtcagta tgcgccatca
     9481 ggtttacagc atggtggcgg aggggaagaa cgaagtcgaa atcattggct ggatgaccga
     9541 acgctacgga gattttgttc gctataaccc accgttaacg ggtcagacgc tggtgttgtg
     9601 ggcgctgcca gtggtgttgt tactgctgat ggcactgatc ctctggcgag tgagggcgaa
     9661 gcgatgaaag agcccaaaat accggtgaaa atgctgacaa cactcacgat tttgatggta
     9721 tttctctgtg tcggcagtta tctgttaagt ccaaaatggc aggcggtaca tgcggaatat
     9781 cagcgtcagc gcgatccgct acatcagttt gccagccagc aaaccccgga agcgcagctt
     9841 caggcattgc aggataaaat ccgcgctaat ccacaaaaca gcgaacaatg ggcgttactg
     9901 ggcgagtact atctgtggca aaacgattac agcaattcgc tgctggcgta ccgtcaggcg
     9961 ttgcaactac gtggtgagaa cgctgaactg tatgtggcgc tggcgacggt gctttattac
    10021 caggccagcc agcatatgac cgcccagact cgcgcaatga tcgacaaagc cctcgcgctg
    10081 gactctaacg aaatcaccgc tctgatgctg ctggcttccg atgcgtttat gcaggcgaat
    10141 tacgcgcaag ccatcgaact atggcaaaaa gtgatggatc tcaactcacc ccggatcaac
    10201 cggacacagc tggtcgagtc gattaatatg gcgaaattgt tgcagcggag atcggattaa
    10261 tcatctggtg atgaagatta tgttattgag ctgcataaaa aataatcgaa tgaacatatg
    10321 ccaaaaataa tcactaatca gtattattgc agattaaaca aataaaaatc tttccataac
    10381 aaatggttat tcattaatcc tgacataaac tcgcgttatt atgcattaat gcagcgaaaa
    10441 gctctgttgt taaagggttg cgcaacatac cgcgcaaatg atactgatca taagcgttaa
    10501 aaaaatctac aaaccaacgc aacacaattc atgcctggca gtatgtcacg ttctcgcgtt
    10561 tctgaacggg gaacggcgct ccattgagga agttattctg atgaaaaata taaaattcag
    10621 cctggcctgg cagattctgt ttgctatggt gctgggcatt ctcctgggaa gctacctgca
    10681 ctaccatagc gacagccgcg actggctggt cgtcaatttg ctctctccgg cgggtgatat
    10741 cttcatccat ctgattaaaa tgattgttgt gccgattgtg atctccacgc tggtggtggg
    10801 tatcgcgggt gtgggtgatg ccaaacagct cgggcgtatt ggcgcgaaaa ccattattta
    10861 cttcgaggtg atcaccaccg tcgccatcat tttggggatc actctggcga acgtcttcca
    10921 gcccggtgcc ggggtggata tgtcgcagct ggcgaccgtc gatatctcga aatatcagag
    10981 cactacggaa gcggtacaaa gcagttccca cggcattatg ggcacgattt tgtcgctggt
    11041 gccgacgaac attgtggcgt cgatggcgaa aggcgaaatg ctgccgatca tcttcttctc
    11101 ggtgctgttt ggtctggggc tttcctccct gcccgcgacg catcgtgaac cgctggtgac
    11161 cgtgttccgc tccatctctg aaaccatgtt taaagtgact cacatggtga tgcgttatgc
    11221 accggtgggt gtgtttgcgc tgattgcggt gacggtggct aactttggtt tctcttctct
    11281 gtggccactg gcgaaactgg tgctgctggt gcatttcgcc attctgttct tcgcgctggt
    11341 agtgctggga attgtggcgc gcctgtgcgg gttaagcgtc tggatcctga ttcgtattct
    11401 gaaagatgag ctgattctgg cgtactccac tgccagctct gaaagcgtac tgccgcgaat
    11461 tattgagaag atggaagcct acggagcacc ggcgtcgatc accagcttcg tggtgccgac
    11521 cggttactct tttaaccttg atggttcgac gctgtatcaa agtattgccg ctattttcat
    11581 cgctcagctg tatggcattg acctgtccat ctggcaggaa atcattctgg tgctgacgct
    11641 gatggtgacc tcgaagggga ttgctggcgt gccgggcgtg tcgtttgtgg tgttgctggc
    11701 aacgctgggt agcgtaggta ttccgctgga aggtctggcg tttattgctg gtgttgaccg
    11761 tatcctcgac atggcgcgta ctgcgctgaa cgtggtgggt aatgcgctgg cggtgctggt
    11821 gattgccaag tgggaacaca aatttgaccg caaaaaggcg ctggcttatg agcgtgaagt
    11881 gctgggcaaa tttgataaaa ctgcggatca ataattgaag attgccgggg ttatccaccc
    11941 ggcaatgtgt ggatgcctga tgcgacgctg gtgcgtctta tcaggcctac gccagacagc
    12001 gcaatagcct gatttagcgt gattttgtag gtcggataag gcgtttacgc cgcatccgac
    12061 atcaacgcct gatgcgacgc ttaacgcgtc ttatcaggcc taccttaacc gttggttaat
    12121 ttttcaaact cttcacaccc ggtatcaaac ccttccatac agctcaggtt caaccagtgc
    12181 agcgcctttt gtttgttctt ctcgataaaa ccttcttcac cgtttaagaa catcattccc
    12241 gcccagtact cggaataacc ggttcgggaa attgcagagc tgcgtttgaa atactaggtt
    12301 gccttgtcat cgtcggcctt aatacccacg ccgttggcgt aaatcaaacc aagcagcatt
    12361 tgggcatcga cggcagagtc gttctccaga tcttccgagg cttttctaac agcgaaatcg
    12421 cctttggata atccggttta cccgcttgag tatttaccag aatatgcgcc agcgttactt
    12481 caccttcttt actgcccgct tgcgccgctt tctctgcaag aacttttgct tgtggatagt
    12541 ccagactgcc cggattggtg attttgatct gcgccaacag cgcgcaggca tcagcatcac
    12601 cgctgtcggc ggctttctgt gcccaatatt cggctttgct caaatcgccg gagctaaacc
    12661 agctgtcggc aagaaaatat tgtgcgcgtc ggtccccggc ctctgctgcc tttaaatact
    12721 ggctgccagg ctcggagtcg ttggcgtggg caaagaatgt caaaaacaac atcaatgcga
    12781 taattttttt cattttgata atacgattgg gtagggagta accagtataa agagttcgaa
    12841 tggataaaaa aacagcctcc gaaaggaggc tgtagaaagg actgtattac gccagtgccg
    12901 cttcgcgcag gcgagttttc aacttgttgt actcgtcaat cacgtactgc tcggcggcgc
    12961 gctgatcggc gatcggctcg acgcgcacgg cgcagtattt gtattccggc gttttcgtaa
    13021 tcgggcttaa gttttcggta accagctcgt tacaggcacc aatccaccac tggtaggtca
    13081 tgtaaatcgc ccctttgttc ggacgatcgc tgacctgcgc acgggtgata attttgcctt
    13141 tacgcgagtg cacccaaacc aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg
    13201 tattgatttg tgcgtagcca ggttcatcag ccagcgccgc cagtgccgca cagttaccgg
    13261 tcatcgaacg gcaagagtag tgaccaactt cacgcaccgt tgacagtacc atcgggtact
    13321 cgtcggtgag tttgtcgatt ggcgctaccc agtcgcaggt gaagaactgc gccagaccgt
    13381 tcggggtatc aaacttctct ttaaacagat aagaagtccc ctgatcggca tctgaagtat
    13441 cgcggcaagg ccactgaatg aagccctgtt cgcccatttt ctcgtaagtc gcaccgtaga
    13501 aatccgggca cagatgacgc aactcatccc agatctcctg ggtgttgttg tagtgcatcg
    13561 gataacccat acgggtggcg atttcactga tgatttgcca gtccgttttc agatcccatt
    13621 tcggttcaac tgctttgaag aaacgctgga agccacggtc agccgcagta aacacgcctt
    13681 catgctcgcc ccacgacgtt gacggcaaaa tgacatccgc cgccgacgcg gttttggtca
    13741 taaagatgtc ctgaacgatg accagttcca gatcttcaaa ggctttacgt actgccgaca
    13801 gctccgcatc agtttgcagc ggatcttcgc ccataatgta cgcggcacgc acttcgccat
    13861 gcgctgcgcg gtgcggcagc tcgctgatgc gatagccggt atgagcaggc aggctttcca
    13921 cgccccaggc tttggcgaat ttctcgcggt tagccggatc tttcacgtac tggtatcccg
    13981 gatacgtatc cggcagcgcg cccatatcgc aggcaccctg cacgttgttc tgaccacgaa
    14041 ccgggtttac acccgcatgc ggcttaccga ggttaccggt cagcatcgcg aggctggtca
    14101 gagaacgcac ggtttccacg ccctggtaga actgggttac acccatgccc cacaggatgg
    14161 cggcgctttt cgcctgggca tacatccgtg ccgcctgacg aatctcactg gcgctgacgc
    14221 cggtgatatc ttcaaccgac tccggcgtgt agccttcaac gattttacga tactcttcaa
    14281 agccttctgt acgggaagcg acgaacgctt tgtcgtacag attttcttca ataatgacat
    14341 ggcccatcgc attcaacagc gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag
    14401 caatgcgcgc ggtttcaatt ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt
    14461 tagcgttaat tacgtgattc gccatgattg ggtgggaatc cgccgggttg tacccgaaaa
    14521 cgaacactaa atcggtatta tcaatttcgt taatagcatt gctcattgcg ccattaccga
    14581 ccgattggtg cagacctgca accgatgggc cgtgtcagac acgagcgcag cagtcaacgt
    14641 tattggtacc aataacggcg cgcgcaaatt tttgcattac atagttggtt tcgttacccg
    14701 taccacgcga ggagccggtc gtctggatgg catccggacc gtacttctct ttgatggcgc
    14761 tcaggcgctc ggcaacgtaa ttcagtgcct catcccagga aacaggttcg agtttgccgc
    14821 cacgctggcg acggatcatg ggggttttca ggcgcggggt caggatctgg gtatcgttaa
    14881 tgaagtccca gccataataa cccttcagac acagggtacc ctggttggtt ttcccctgcg
    14941 ctgcctccgc ccggacgatt ttgccgttat cgacgaccag gttgattttg caacctgatg
    15001 cgcaataggg gcaaaccgtg acgacttttt tcatcggtct cgctccagtt aatcaaatca
    15061 cgcatacgcg ctctcgacta cagtatgcat cttttatgcc acattttatt tggggtcatt
    15121 ccctgatatt acgggcacta tttattcaaa actctgacga aaaacaggct gtcgtcagtt
    15181 ttgacgtgac gaaacgaaat accgcgtgac agccatcacg cggcagacat tttatttttt
    15241 ctcgacgacg ggagccgcct gatacccgcc gcccagcgat ttcatcaact gaatgctgtg
    15301 gatcacccgg cggctgtcca gcatcagtaa tgacatctct tcggcaagca ctggcaaccg
    15361 ggcttcggtg gcctgtaagc ggctggttaa gccgcgctga taggcggcct cggcagcgcg
    15421 ctgggtgaag cgcgttgctt ccacgcgttc agcctgcatt tctcgttcgt cgttgagcgt
    15481 ttgcagacgc gtgccgttga cggcaacgtc acgcaccgcg ttcagtactg actggttgta
    15541 acgttcaatc atcatgttgc tggcggcgcg agtgccttcg agattggcat tcaaccgtcc
    15601 accgtcaaac agcggcaatt tcagacccgg gatgaagttg aactggcgac tggttttttt
    15661 gaataaggta tccaggtgga tggagtccag accgaaaaac gctttgatat caaagctcgg
    15721 gtagaacagt gcccgcgcgg aatccacctg atctaatgac gcctgaacat accagcgcat
    15781 ggcttgcaga tccgggcgtc tggcgagcaa ctcataagag agtgtcgccg gaatgccggt
    15841 ctggactcgt ggtaatgcca ccggtttgat ctccggcata tcgctggccc ccgcgccaat
    15901 caatgcacgc agagattctc gcgtttcggt gatttgccct ttgacggcag caatttgttt
    15961 atcgaccgcc agaatctgtg cccgcgcgcc gtggaaaggc acttgtgctt ccagaccgtg
    16021 cgccacttta ctctggtgcg ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa
    16081 cagatcgagc atctgatagc tggcctgcat actgtaataa agctgcgcta tgcccgtggt
    16141 cagcgatagc tctactgctg cggtttctgc cagcgcggca ttatgcgcgc caatggcggc
    16201 ggcaaccgct gagcgatgca caccccacaa atcaagatcc agtccggcaa acaaacctac
    16261 tgtggcttcc gtatagtacg gcccgtccat acccagtgag ggcgcatcca tcgcataagg
    16321 gcttaaaaag ccgttcgccg agacgcgttg gcggttgagc atccctaacg ctgcgacctg
    16381 taattgtgaa ccggcatcta acaaatcggc ctgggactgc gctttttctt cccgcagttt
    16441 cgcttcggcg agggtgtgtg aaccacttag cgtccgttgg atcagcgcat ccagctgcgg
    16501 gtcattgagt tgtttccacc actgcgcctg cggccagccg gagctggcaa gatgaatatc
    16561 gtcggccagt ttgatttgtt ccggtttgag ctgttgatga ggtgcagaat ccttacgtac
    16621 cagggcacag ccggaaatca gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga
    16681 aagttgacga ttgatcattg cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc
    16741 ggggctgtcc agtctggcaa gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat
    16801 aaggtgggca ggatggcctg cgaggcgggt gtctcttcaa gcgttatttg aggagaacag
    16861 ctgagtgcgg ttgccagacc ggcagcgtat ttttccaggg cgtcggcaaa ctgacctgct
    16921 ctgtccggct gtagtgcgtt atccagcgcc tgcgccgagt tccaggtggc atcccaggcg
    16981 tgaagaagat cgcggccctg acgaataacc gtttgcgaac gttcaatcag taaggcgcgt
    17041 tcttcgctgt ccagttgacg ctccagcgcc acgcgttggc acatctcttc acaggcatta
    17101 aacgccgcat gcagacctat acgaatttgc agataagtgc gcagagccgt gacttcctgc
    17161 tggcgtggga tccgcattac tttacttagc atacccagcg caccggctag tttttgcggc
    17221 agagtgcgcg cttcactttc aggccagaca aaggtgtaaa tcaccgcgga caccacggta
    17281 ccaatgatga tacccagggc gcgatcgcga atttccacca ggtcgtacac tgggccaaaa
    17341 acgttttcga gcgtggcgag cgcgaaggtg acgaccatct gtgtgccgat ataagaagag
    17401 cgttcagagc tggtggcaat ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc
    17461 aattcgacaa tattgtccag ccagggcatg accagtagcg tgaataacag cgccagaatc
    17521 gcgccgcaaa aggccccgcc aaaacgcagc accatcttct ggtacgacga accgacattt
    17581 ggattagcga cgatcacgca ggtcagcata caggtgtgaa tgccttccca gtccacgccg
    17641 ctgtaaaagg tgtaacagat caaacaggcg agcaatgttt ttaccgcgta gcgtatatag
    17701 tctggattgg taaaagcatc ggcgaccatt gatggcggtt tggcggcggg cgttggcggc
    17761 gtattcgggt tcatctggcc cagttgtaac aacgtctggc agatattctc caggttacat
    17821 tcccgtgccg ccaccgcttc actttcactg atccgccagt cgctttgcca gcactgacct
    17881 tcagcaacgg catgctgcag cttgttgatt tctgaggcta atttttgccg aaattcaata
    17941 attgcctgag aatcagcaaa agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag
    18001 gttaccgttg ccacgcagct ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc
    18061 gcgagacaaa agacattcag tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg
    18121 ggtagcggtg cgaggctgtc tgtcaggtga ctaatggcat catcaagccg atcattaagc
    18181 gcctgatgca tttgcgaaat ggcacgactg ggaaaccaca gcacgccgat taacgtcatc
    18241 agcaaggttg gatagaggcc aacaacgata caccacagcg ttaagcgcac gaccacttcc
    18301 ggatagtcga gcatggcggg gaaggtttgc ccgtaaatgg cgacaatggc gacagcgaaa
    18361 aagaccagtc ccagccgatg ggtgcgcatc aaaaacatgc agcccatcag gatcggcccg
    18421 gcgatgatca atcggatcaa cggttcgccg tatgaccatt tgtagatcaa aaacaggctg
    18481 ccgatctcca gcaccgtggc aaccacaaac aagatcgcca caaatttggt gtaaaacgcg
    18541 ttcgactgaa taccgtaaaa cagcactgcc agcgataacg ccacaaaagg gatctcaaaa
    18601 gtcatcgaga tcagaatcac cagcaggcag cctacccaga gttgcacgat ctgcggcacg
    18661 cggccgggtc gccgctcgct taactcttca tgaaagaacg ccagcagcct gaccaccggt
    18721 aatggcaggg agttgagcgc gctcattatt gcggctcaag attagcgact gccgaagcgc
    18781 cgatgcggaa catttccggg tcaggtttat cgaccatgat tttgaccgga aaacgctggg
    18841 caacgcggac ccagttaata gagcgagaaa ctttcggcag gccgcccagc accaggccgc
    18901 cgtcattcgg tagcacaccg tagccaatcg aatccacttt accctcgaag gttttgccgc
    18961 tgtcactcat caggcgaatc gttgcgggtg tacctgagcg aatatttttc agatcggttt
    19021 cgcggaagtt ggcgatcacg taccagtgac gagtgtcgat tagggtaaaa atagggcgca
    19081 tggcagaagc aaattgcccg acggaggttt tgagggaaat gacccggcca tcaaacggcg
    19141 cgcgaacggt cgccatttcc agatgcagtt tggtcagggc aatatccgct tcgaccgccg
    19201 cacgctgggc aactaataca tccacgccgc tgacggcgct ggcggctgac tgcgcctgta
    19261 acaatacggc attaagatcc gcttctgcgg cgcgctgcgc cgttcttgca cggtcaacat
    19321 cttccgctga gacaaaacct tctttcagta atggctcggt gcggcgtaat gtatctgtgg
    19381 cctgtttcgc ggcggcacgt gctttttcta ccgtggcatt aaccgagtcg gcaccaaact
    19441 gttgggcgtc aacgctacgc tgggtgagca taatttgctt atccagcgcc gcgagggagg
    19501 cttcagcttt cgccagattg gcttcgtacg ggcgcgggtc aatgcggaac agcaaatcgc
    19561 cctgtttgac tgcctggttg tcggtgaccg ccagttctac aatgcggccg ctgacttccg
    19621 gcaccacatc aatggtatct gctgacgcgt aagcgtcatt agttgatggc gcactgtcta
    19681 cgcgccagat aacgaaaaca agggcaacca gcgccaacgc aaccactaac agagcaggga
    19741 atttactgcg aggagctttt ttcggcgtac tttccatgag cgcgtctcaa atagattaga
    19801 aaaatgccag ccagaacagc atggcgtaga cagcaaaaag ggcggtataa ataattccga
    19861 caaatgccag attgatattt gcccgctgaa taattcgcct cgtgattagc gtcaaaataa
    19921 ggctggcaat ggcgcagaaa aaccagctgg gataataagc gccgatcacc gggattgccg
    19981 gagataatga gcatcccgaa accatgaaga cgggaattat ccaggctgtt tttttgagtt
    20041 gcatcgccat gcgagagaga actgttggca tattaaatac atcctgtttg ctgcgaacaa
    20101 tatctgtagt gaatatcaat atccattgaa ggcgtcagta tggctatttt cttgatgaat
    20161 aaaaatagct ttattcagcc accgcattca taacttatat attttatgtg ttaattgatt
    20221 gtatgtattt ttataataaa tgcttttggg ggcttaagcg cccccaaaag tgagtggcat
    20281 taatttgggg ttacgatatt cacccacaaa tcgaaattat ccagacaggc aataatttct
    20341 tccagtttcg cgccattgcc aataattttg gctttcttcg cttttaccag atccgccatt
    20401 ttggcttgtc ctgtcagcac ggcgtgcaga tcttcacggc tgatgtagaa agaggcgtcg
    20461 gcttgcggtt gcagtgtttt acggtagtta agcacgctat cgttcagcgt caggttgagg
    20521 ttatcgccgt tgctcatatt gaagttcagg ctgatatttt tacccgcagc tttcgcgcta
    20581 tcgaggcgaa cggacataaa gtcgaacagc atttcgaccg acatcccgcg aatggtgtcc
    20641 ggggaaccgg tggtgccgtg gctgaactta tgcacccctt cgcgcagctc tttcgcgccg
    20701 gtcaggtaga aaccgcgcca ggtggcggac tcggcctgat agcccagctg ttcaaagtta
    20761 ttcgcttgca gattcttcgc gacctggtca cccgggttgg cggcaatcac ctgtttcagc
    20821 agttctgccg accagcggta atcaccttgc ttgttcgctt cttgcgccag gctgatgaca
    20881 cgggcagaac cgcccagcgc ctgcacgtaa cgtttaccca tctccacctg accatacgga
    20941 tgcaggttag ccgggttacc gtcgtaatag ccaagataga agttatacac cgcgcgggcg
    21001 ttgtggctga cagaaccgta atagccacgg ctggcccagt tattggcaag tgcaggcggc
    21061 agtttaatca tgtcgccgat ttcattcatg gtgtagccct ggttcgccag gtgcagggtc
    21121 tggtcgtgaa tgtacttgat agtatcgcga tatttaccaa tataatcatt gatatgctta
    21181 ttgccccgga ccggccaggt gtgcggcata aacaggactt ccgcgtcgtt accccacata
    21241 tccagcgttt cgttcagata ctcggtccac ttgctggtgt cgcgggtttt cgcgccgcgc
    21301 agagtgtaga agttgtgcag ggtatgcgtg gcgttctcgg cggtacacag cgctttcagg
    21361 gccggaatat agaagtgcat ttcggctggt gcttcgctac ctggggtcat caggaagtca
    21421 aactccaggc cgtcgataat catcttctca ccggttttgg tgatggtttt gttcggtgcg
    21481 atgatgctcg ggtagcccga tgccagcgtt acgccaagac cattgccgat gttaccctga
    21541 gggttatgcg ccagcagcag gccgtaggag taaagtgcac ggtggctcat aatattaccc
    21601 gccagtacgt tttcgctgat ggcttcgtcc ataaagccag caggggcgat aacctgcact
    21661 ttaccggatt tcacatcagc ttcagagata atgcctttca cgccgccata gtggtcggta
    21721 tggctgtggg tataaataac cgcaacaatc ggtttttgcg ggcgattttg gaagtaaagg
    21781 tcaagggcgg cttttgcgct cggtggcgtc accagcgggt cgatgacaat aatcccggta
    21841 tcaccttcga taaaggtgat gttggagata tcctgaccgc gaacctggta cattctgtcg
    21901 gtgactttaa acaggccgga aataccgttt aactgcgact gacgccacag gctagggtta
    21961 acggtttcgg gcgcgggcgc attgatatca aatttataat cttccccg
//
LOCUS       DAJTOR010000057        21661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTOR010000057 DAJTOR010000000
VERSION     DAJTOR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.057"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..420
                     /locus_tag="OO249_001910"
     CDS             <1..420
                     /locus_tag="OO249_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCR5636225.1"
                     /translation="MHILAKANPRCGVKETILTTLANPDAAGLRFMVNICVWCRVPLT
                     FEEIRQLQENAPSVKHACAYCRLYHNLNKWDGLILLLQSQHKLTEEFAGKQLAIWQRN
                     FNLSGIQPNALQRQQLQALFTRNPELHNRLWGYIPFK"
     gene            741..2102
                     /gene="yegQ"
                     /locus_tag="OO249_001911"
     CDS             741..2102
                     /gene="yegQ"
                     /locus_tag="OO249_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5636226.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREYFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGVAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2432..2749)
                     /locus_tag="OO249_001912"
     CDS             complement(2432..2749)
                     /locus_tag="OO249_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636227.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARSRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            3163..4061
                     /gene="yegS"
                     /locus_tag="OO249_001913"
                     /pseudo
     CDS             3163..4061
                     /gene="yegS"
                     /locus_tag="OO249_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
     gene            complement(4143..4916)
                     /locus_tag="OO249_001914"
     CDS             complement(4143..4916)
                     /locus_tag="OO249_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5636228.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(5022..6062)
                     /gene="gatD"
                     /locus_tag="OO249_001915"
     CDS             complement(5022..6062)
                     /gene="gatD"
                     /locus_tag="OO249_001915"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5636229.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITIGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGSMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(6110..7465)
                     /locus_tag="OO249_001916"
     CDS             complement(6110..7465)
                     /locus_tag="OO249_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5636230.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNIDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSVSLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFITWRRARGFIKQEKAVLA
                     E"
     gene            complement(7469..7753)
                     /gene="gatB"
                     /locus_tag="OO249_001917"
     CDS             complement(7469..7753)
                     /gene="gatB"
                     /locus_tag="OO249_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5636231.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(7784..8236)
                     /gene="gatA"
                     /locus_tag="OO249_001918"
     CDS             complement(7784..8236)
                     /gene="gatA"
                     /locus_tag="OO249_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5636232.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(8246..9508)
                     /gene="gatZ"
                     /locus_tag="OO249_001919"
     CDS             complement(8246..9508)
                     /gene="gatZ"
                     /locus_tag="OO249_001919"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5636233.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIVRGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(9537..10391)
                     /gene="gatY"
                     /locus_tag="OO249_001920"
     CDS             complement(9537..10391)
                     /gene="gatY"
                     /locus_tag="OO249_001920"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5636234.1"
                     /translation="MHVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATEPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(10699..11751)
                     /gene="fbaB"
                     /locus_tag="OO249_001921"
     CDS             complement(10699..11751)
                     /gene="fbaB"
                     /locus_tag="OO249_001921"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5636235.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVVSTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            12008..13285
                     /locus_tag="OO249_001922"
     CDS             12008..13285
                     /locus_tag="OO249_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5636236.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPGTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFCESDNEITAIKVDDRDIALTQGEVK"
     gene            13282..14286
                     /locus_tag="OO249_001923"
     CDS             13282..14286
                     /locus_tag="OO249_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR5636237.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSITRHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            14283..15248
                     /locus_tag="OO249_001924"
     CDS             14283..15248
                     /locus_tag="OO249_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5636238.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15222..15968)
                     /locus_tag="OO249_001925"
     CDS             complement(15222..15968)
                     /locus_tag="OO249_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5636239.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(16020..16838)
                     /locus_tag="OO249_001926"
     CDS             complement(16020..16838)
                     /locus_tag="OO249_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5636240.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQKVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(16903..17703)
                     /gene="thiD"
                     /locus_tag="OO249_001927"
     CDS             complement(16903..17703)
                     /gene="thiD"
                     /locus_tag="OO249_001927"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5636241.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIESDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQYV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(17700..18488)
                     /gene="thiM"
                     /locus_tag="OO249_001928"
     CDS             complement(17700..18488)
                     /gene="thiM"
                     /locus_tag="OO249_001928"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5636242.1"
                     /translation="MQVDLQSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLPHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(18523..18619)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20200..20493)
                     /locus_tag="OO249_001929"
     CDS             complement(20200..20493)
                     /locus_tag="OO249_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636243.1"
                     /translation="MYGQDTTAGGNLKKDSPLGKLWDFLHSPDLPTNPDFAKPADKTG
                     WVSSYSNPTYVPPTPTITAPQLKVDMAGNPLMTPPAWLNQPLNVNVNVKVQDG"
     gene            complement(20457..>21661)
                     /locus_tag="OO249_001930"
     CDS             complement(20457..>21661)
                     /locus_tag="OO249_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR5636244.1"
                     /translation="GKIMTTGQDFESALSGLTAITGSTDKAAKEFDYLRDQSNRLGLD
                     LLKTSRDYTQFAAAVGDKLPKDQMRSIFEGASEWGLVTGASADEASRALKSLNQMMSK
                     GTVMSEELKGQLSEALPGAVGLFVKALNQMKGTTNLTEKDLFDLMQQGKLFSKDILPE
                     VAKQMKAAARNGGALDKAMKSNRASWQRLQTSMQNAMNIFFASGFGNELTSAFDAISA
                     AVNGSGGAFEMFGHIAGKIVEGATDAFTELHDTVILTFRIIEYYASKMGITGEQLKAW
                     GEMAAYAAGVAMFAGSIWKLGVAFKWLLTMLNPLTKLLGVMKGIAAIGGIEAATTGGS
                     GGEGKDKPNKPGRGPKVGKVGGAAALSGGPVGWSIAVGSVGGDYAWDKVMTLTTPCMD
                     RTPPPGAI"
BASE COUNT         5396 a         5593 c         5176 g         5496 t
ORIGIN      
        1 atgcacattc ttgcaaaagc taatcctcgt tgtggcgtca aagagactat tctgaccacg
       61 ctggctaacc cggacgccgc cgggttgcga tttatggtta acatctgtgt atggtgtcgc
      121 gttccgctca cgttcgagga aatacgtcag ttgcaggaaa acgcgccgtc agttaaacat
      181 gcttgcgcat attgtcggct gtatcataac ttgaacaaat gggacggttt aattcttctt
      241 cttcaatcgc aacacaagct aactgaagaa tttgcaggta aacagctcgc gatatggcaa
      301 cgcaatttca atctttcggg cattcaaccg aatgctcttc agcgtcagca acttcaggcg
      361 ctttttaccc gcaatccgga acttcacaac agattatggg gttacattcc ttttaaataa
      421 cttcgaatta tagacttacg atgacaaatg tacctgctga cgagcggaac caaaacgaaa
      481 caatacgtga agctttacgc ttgctacgag aaaaacttgc cgagggacta cgcagtggcg
      541 aagcccaacc gtgggaaaag gatctgtttt tacataaagt gaaggccggt aacaaataaa
      601 tcccccagtc ttttccctca catttgcact ggagccaacc ccgccgattg cgctacaatg
      661 cccgccctta aagtgggggc actcccctaa ccgcttcatc aggtgaagcg gatctgacct
      721 gtcatcagaa cgagagaatt atgtttaaac cggaactcct ttccccggcg ggaacgctga
      781 aaaatatgcg ttacgctttc gcttatggcg cagatgctgt ttatgcgggc cagccgcgtt
      841 actccctgcg tgtgcgcaac aacgaattca accacgaaaa tcttcagctc ggcatcaatg
      901 aagcccacgc gctggggaaa aagttttatg tcgtggtcaa cattgcaccg cacaacgcca
      961 agctgaaaac ctttatccgt gacctgaaac cggtggtgga aatggggccg gatgcgctga
     1021 ttatgtccga tccagggctg attatgctgg tgcgtgagta cttccctgaa atgccgatcc
     1081 acctctcggt gcaggctaac gccgtaaact gggcgacggt gaaattctgg cagcaaatgg
     1141 gcctgacccg cgtgatcctc tctcgcgaac tgtcgctgga agagattgaa gagatccgca
     1201 atcaggtgcc ggatatggag atcgagatct tcgttcacgg tgcgctgtgc atggcctact
     1261 ccggtcgctg cctgctctct ggctatatca acaagcgcga tccgaaccag ggtacctgca
     1321 ccaacgcctg ccgctgggag tacaacgtcc aggaagggaa agaagatgac gttggcaaca
     1381 tcgtacacaa gtacgagccg atcccggtgc aaaatgttga gccgacgcta ggtatcggcg
     1441 cgccaaccga caaagtgttt atgatcgaag aagcccagcg tccgggcgag tatatgaccg
     1501 cgtttgaaga tgagcacggc acttacatca tgaactcgaa agatctgcgc gccatcgccc
     1561 atgtagaacg cctgaccaaa atgggcgtgc attcgctgaa aatcgaaggc cgtactaaat
     1621 ctttctacta ttgcgcacgc accgcacagg tttatcgtaa agctatcgat gatgccgctg
     1681 cgggcaaacc gttcgatacc agcctgctgg aaactctgga aggtctggcg catcgtggct
     1741 ataccgaagg tttcctgcgt cgtcatactc acgacgatta tcagaactac gaatacggtt
     1801 attcagtttc tgaccgccag cagtttgttg gtgagtttac cggtgagcgc aagggggacc
     1861 tcgcagcggt agcggtgaaa aataaattct ccgttggcga cagccttgag ctgatgacgc
     1921 cgcaaggcaa cattaacttt acccttgagc acatggaaaa cgccaaaggt gtagcgatgc
     1981 cggtcgcacc aggcgatggt tatactgtgt ggctcccggt cccgcaggat cttgaactaa
     2041 attacgcgct gctgatgcgt aatttctccg gggaaaccac gcgtaaccca cacggtaagt
     2101 gattaatttc gattattttt cccggatgga aaattcttag aaaccgatca catacagctg
     2161 catttattaa ggttatcatc cgtttcgctg aaaaacataa cccataaaat gctagctgta
     2221 ccaggaacca cctccttagc ctgtgtaatc tcccttacac gggcttattt tttatgcata
     2281 agccctatcc ctggtcaccg tcttccattg accacatcga tagaatctcc cttcatagca
     2341 cgatgccttt cacgtaacgg catcgtgctc gcacaggttc cggctaagca caaccagaac
     2401 gcgcatgttt gatgcttacc aaaaaatatt ctcactctcc acatttgaat gtcagacgag
     2461 cgacaccatg taatcctaca ccttctgtct tcagctcaac tatttgcatt tttttgccct
     2521 gagtaacaca gaaatgagtt gcatcatttt ttactatatt ttctgcacca gatattctac
     2581 tcctggctaa agaagcttcg gcttcggtgt agtattggtt atcgagttta cgctgaatat
     2641 tacttttata tgcaagacca aatttaccga tacttgtctc atcattatgc acagcacaac
     2701 cagacataat aaaaatacta attaatgata tagcagctat ctttttcatc tcacctttcc
     2761 ccattaaata ccaacgacac tctctagtgt ttaaatataa taatggctga tcattatatt
     2821 tgaataggat tataataaat gttctgtaca acatttccta cataagtagg acttacagac
     2881 atgaaggccc tttccaggta actccatggg gctttaatat atttggaata gtatatttaa
     2941 gatgccactg tttggttgaa tattaggcat atgctctttt ttgaaattta tcggtggcta
     3001 ccgttaatat tcgctgttcc cattgcaagc tcctggtggt aaccactgaa tcctccatac
     3061 ttgaactgac tttttatcct ccgactttca tcctgttctg actccacctt ttcttttctg
     3121 ctctacacta tctacagacc aatcataaag gcacatacga tcatggcaga atttcccgcc
     3181 agcttactga ttcttaatgg caaaagtact gacaatctac ccttgcgcga agcaattatg
     3241 ctgttgcgtg aggaaggaat gacgatccat gtgcgggtca cctgggagaa aggtgatgcc
     3301 gcacgatttg tagaggaggc ccggaagttg ggcgtcgcaa cggtaattgc cggtggtggc
     3361 gatggcacca ttaatgaagt ttctacggcg ttgattcagt gtgaggggga tggcataccc
     3421 gcgctgggaa ttttgccatt aggaaccgcc aatgattttg ccaccagtgt agggattcct
     3481 gaggcactgg ataaggcgct gaaactggca attgccggta acgccattgc gatagatatg
     3541 gcgcaggtca acaaacaaac ctgttttatt aatatggcga caggcggatt tgggacgcgt
     3601 attaccacag aaacgccgga aaaattaaaa gccgcgctgg gtggcgtctc ttacatcatt
     3661 catggcttaa tgcgcatgga tactctgcaa ccggaccgtt gtgaaatccg cggtgaaaac
     3721 tttcactggc aaggtgacgc cctggtattg gtattggtaa cgggcgtcag gccggtggcg
     3781 gtcagcaatt gtgtccgaac gcgttaatta acgatggctt gctgcaactg cgcattttta
     3841 ccggcgatga aattcttccg gctctcgtat caaccttaaa atctgacgaa gataacccga
     3901 gtattatcga aggcgcttcg tcgtggtttg atatacaagc cccacacgaa atcactttta
     3961 atcttgatgg cgaaccgttg agcggacaaa actttcatat tgaaatactt ccggcggcgt
     4021 tgcgttgtcg attaccacca gattgtccat tattgcgtta atcaaactat tctctcttga
     4081 accagggaga atccgaaatg ccggatgcgg tgtaaatacc ttatccggca tacagaacaa
     4141 tactacaccg taattaattt tacccgtgtc ttctgcaatg ccagtttatc cacctctgat
     4201 acagcatcat cggtaataat aatgtcaatt ttttcgattg gcagtacctg attaaaaccg
     4261 cgacggttaa atttcgatga atcgagaacg gcaatcactt tattagcggc tgtgaccatc
     4321 gcaccgctaa cggaataacc ctcattaaag gtcgtaatac cattaaccgc atcaatacca
     4381 tcagcaccga caaacattaa atcggcatta atatcctgca gggaacgttc agcaatagat
     4441 ccatgcatag atcgcgtttt atggcggacc gtgccaccgc agacgaccag agtaatgtct
     4501 ttattttcgg aaagggcaaa cgctgccggg agactgttgg taatcaccgt gatattttta
     4561 gcggtcatta atccttcggc gataagcatt gtggtacttc cgctgtcgag aataacggtc
     4621 atcccttcat ggatcatttt gactgccgcc tgcgctattc tgttttttgg cgcgctggcg
     4681 agctgaaatc gctctttaaa cccgacttcc tgggtctcgg tttcactatt accggacatt
     4741 attttcgccg caccgccatg aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa
     4801 tcggcacgga ttgtcgcttc cgaggcagca aatactcccg ccagatcctg aacaagcacg
     4861 gttccctgtt cattcactaa ttggatgatc ttattccttc gctcgaatga attcatcgtc
     4921 atttacccat attcaattgt ggctagtgta aacgaagggg gaccattggt gaatgatctg
     4981 ttttgcacaa taccgggcca gcatcacgct ggcccgcggt ttcatggaat gagcaacact
     5041 ttgcccggca tggcattacg agcgatgtca cgcaccgcct gggtgaagct ttcaaagctt
     5101 ccacggtgag cgattaatgg ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc
     5161 gtttcccact cctgccccgg ccaagggctg gaatagttca tccaactgcc gataaccgtc
     5221 agctctttac gcaatatttt gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac
     5281 gtgcccacca gcgccagttg ggcatgagga ccggcaatct ctaccgccag ttcgacagtt
     5341 tgcggtacgc cagccgtctc gaggataagc tgattaaaac gcagttcgcg taaaacgctc
     5401 tgcatttgcg gcgcgctcat ttcgctactg ttaaatgttt gcatcgaacc gaaagatttt
     5461 gccagtgcca gtttttctga gctaatgtcg atagccgtca cactctttgc tcccagcgcg
     5521 acagcgcact gaatagccag caggccaatg gttccggcac caataataat aacgttttta
     5581 ttctcacaac cttgcgctaa atgaaaagca tgcagaccaa tggtaatcgg ctcaataaaa
     5641 gccccatcct caataggcat atccgtgggt agagcaaaga cattttttcg cttaacgaca
     5701 atatattcag caaatccacc atcacgccgc gagccaataa aatcatattt tgcgcactgg
     5761 gaataaaacc ctttcagaca ctctggacaa gtaaaacagg gtaataacgg cacacaggca
     5821 accgcatcgc cagggtgtaa atcatcaaca ccggatccca ccgcatcaat atagccgcta
     5881 aattcatggc ctaacgttat tggataataa tgtgcaccat ttttaaatat cctgggtaaa
     5941 tcggaaccac ataagcccga gctggcaatt tttacccgca cctcatcctg atgtttaatt
     6001 tcaggaatga cgctttctgc aacgcgcacg ataccatcag tatcattcac cactgatttc
     6061 ataaaaactc ctgattgtta agggggataa actccccctc agttaaaaat tattctgcga
     6121 gaacggcttt ctcttgttta ataaaaccac gcgctctacg ccaggtaatg aaaataccgg
     6181 tcagataaat tgcgccgata ataatgaaac cgggaatatt ttgcggggag aaaacctgaa
     6241 tcagtaacca ggtaatggga gaaccgccct gatccattga agccaccata cccccggctt
     6301 ttaacgcccc agcattagcc gccagttggg tgtgcaaacc aatagtttgc gtcgcgatcc
     6361 acagagtgat gctcatgatg atgacacccg agattaaggt gcggaacaga tttccacgat
     6421 gcacggcgac tgccatcgcc acaaagaagc cgatggtggc aagatcgcca aacggcagta
     6481 cctgattacc cggaacacaa acagcaatta aaatggtgag cgggataaaa atcaggctta
     6541 ccgataccac cgccgtatgc cccagcagta atgcgggatc caagccaatc aggaactcct
     6601 gaccgccgaa cttcgcctgt aaacgactac gggcctgctt agcgatgggc gttaaaccat
     6661 ccatgatggg tttaatcacc cgtggcatta gcagcatcac tgccgccgtt tttaccgcca
     6721 gctgcaatac acctttgaca tcgtaacccg cgaggatgcc gataatcagc cccatcacaa
     6781 aacccacggt gacaggttcg ccaaaagggc caaatttgcg ctgaatatca tcggcgctaa
     6841 atttaattcg gttaacgcct gggattttct cgatgatagc atcgaccagc accgcaatcg
     6901 gccccatata cgccgacgta ccgtgcggaa tagcaatacc ttccagctca aagaaatttc
     6961 gggtatcgcg ggcaaaccag tcgccgagct tataaacaaa cgccgcgtga attaccacac
     7021 ccgccatccc tatcatccat gaaccggttg ccagatgcag caacgcgccg gtgaaagtca
     7081 tatgccagat attccagata tcaatattta ccacccgcgt catacgggtc agtaacatcg
     7141 ccacgttaac cagaatagca atcggaatcg ccaccagcgc aatttgcgac gcccaggtca
     7201 ttggtgaaga gcccggccag ccgacatcga ccacatgcag attcaggtcg aaattttccg
     7261 ccatcgcttt cgccgccgga ccaatggaat ccagcattaa gccaatcaca aggccaatgc
     7321 caacaaagcc aatccctata tgaagacccg ctttaaagca atcgcctgcc ttcatgccta
     7381 atattttaga aaaaataatg atgacaatcg gcagcatcac cgtagggccg aggtcgagaa
     7441 tataacgcat gacttctgaa aacataggtc acccctgtaa gatagtcaga attttatttt
     7501 gtaatgcttc gataccgaca ccagaaacaa aaggcatgcc gtgaactaac ggaatatcgc
     7561 caaaactacg atccaccctg gcagtagtgc atatcaaatg cacgccatcc atataggttt
     7621 ctatttcatt aacccgacac tggattaatt caacaggaat attatgattc tgacacaatt
     7681 ctttaatttc ttccgccgcc atcgtagagg tcgcaaccgc gcctccgcaa gcgacaataa
     7741 tcttgcgttt cataaatagt cctttttatt gttacagagg gatttattcg tctgaatcta
     7801 aaacatactt tgtgaagtat tcctttaact gggtttcagg aagagtgatt agtgtctcga
     7861 cgatatcggg ctgttgtaac ttgccaaata aacagcgtaa aagtttcaat tgctgctgcg
     7921 gattttccac aatcaacgca ataaccaacg atacctccac gtcgttatca tcatccgctt
     7981 gctgaaaatg aactttattt gttggcctta acagataaat ggctgacgac ttagcatgaa
     8041 tcgcctcaca atgcggtatt gcaatggcgt gctgctcaag cattatcccg gtagggaatt
     8101 ctgcttctct ggcaattaat gcctgtggcc aggtatcatg aaccacacct ttggcgagca
     8161 tctcattacc gatatgggtt aaaacttcgc tacgatcgac aaaagaaatt ccgctacgaa
     8221 caaacaggtt agtcatatac cgtccttatt ccgcacagcc gtagcgatag gcgcgcaaaa
     8281 catcataaat tttatccata atcagctgat gcggtattgc tgataattcc ccggactgaa
     8341 tgcgttcaaa ttgtttggga agatactgac taatcatgcc cagtgggatg tccacgcctt
     8401 caagattcac catcatcgtt tcgacgctat ttttaatccg actatgcggc cagtaataac
     8461 gaatacgatc cgacaggctg taacgaatat ccagtaatga atcgttaaaa cccgtacgat
     8521 aatatttttt ccagtattgc ggttcgtcga gcatcacttc ttcaattacc gccaggcaac
     8581 cgctgcgatt ttcaggggcg ataagttcct gctcaatttg tgccagtgca aatatcgcct
     8641 cgcgtaaagc aaaggttaat gcgggaccga ctttcaatat tgcaaagtga tcgcggacta
     8701 attcccaata agccgtccgg gtctggtaat cggtagaatg tgcttcataa accattcggg
     8761 tgttttctat ccattgcgcc agcggctgcg cttcctgcgg ctgataatgg ataatattgc
     8821 tgtgatcaaa ttccacgccc ggctgcacca cgatggcaat cacgcgtgtt aacgcctctg
     8881 tcagcccacg gacaataaag gccttttgat gcgtacgtaa agtattggcg gcatcttcaa
     8941 catgggtgat gtgtactgac tgaatggcgc tggcctcacc gcccggaacc ggaacttcgg
     9001 tgccaatgac atagctcagt tgctcacgct ggcaatctgt cgccacactt tccgcagcaa
     9061 agcaaagcac agcagctcgt tccgcaaccg tttctggtgc taacggtatg ggatcccccg
     9121 cgcaggacat tgacgcatca agatgaattt tactgaagcc ggcacgaaca tattccttta
     9181 ccagctcgac ggatttttcc atcgccgcat ccgcattttc ttgctgccag cagtttggcc
     9241 ccagatgatc gccgccgaga ataatgcgtt cgcgtgcaaa cccaacttta tcggcaatcg
     9301 taaaaacaaa ttcgcgaaag tctgccggtg tcattccggt ataaccgcca aattgattga
     9361 cctggtttga cgttgcttca atcagcactt tgcgcgtgct gttgcgatca aatgccagcg
     9421 ccgcttcgat aaccaacgga tgggcagaac agactgaaca tatgccgata tgttcaccag
     9481 ctttatgccg ggcaattaac gttttcatgt tttttcctta ttaaatggca ggtgcgttat
     9541 gccctgccct cgcagccaca atcggcaatc actttgctca ccacatcgcg cattgcggat
     9601 ttagccgact gcaaataatc ccggggctcg gtcgcttcag ggtgctcggt caggtaattt
     9661 tttaacgcct gcgagaaggc atttttcagc tccgttgcaa cgttgatttt gcatatcccc
     9721 agtttgatgg tttgctgaat atccttagtc gataaccctg acgcgccatg cagcaccagc
     9781 ggtaagttca cccactggcg aatgttctcc agtctagaaa aatcaagcgc cggtgcgctg
     9841 gcatacatcc catgagccgt gccgatggcg accgccaggg aatcaattcc ggttgcctcg
     9901 gcaaattcac gcgcctgagc ggggttggta taaaaagcat cggcttcatt gacttgcaca
     9961 tcatcttcct ggccgccaag ttgccccagc tccgcttcga cgctgacatc aaagcgatgg
    10021 caaaaatcca ccacctcttt gacccgtgaa atattttgcg caaaaggcaa atgcgaggcg
    10081 tcaatcatga ctgagcgcac gccagaacga accttctgag cgatatcgtc aaatttcgtg
    10141 tgatggtcga gatgaattgc cagtggatgg tgatattgct tcgccatcgc gctgaccagc
    10201 gccaacagat tttctgtacc agcatgagta aatgtgccag gcgttccggc gatgatgacc
    10261 ggcgcatgca ggttggcagc ggtttccacc accacttgca tcgtttcgag attgtgaata
    10321 ttgaatgccg gaaccgcata accgccgcgc tgtgcgttgt tcagcatctg ctttgtcgat
    10381 accacgtgca ttttcatatc ctgtcgtttg ttttcgtttt caaaatataa tgaaattatt
    10441 tgtttttaaa tatcgagata acgatcacaa aaacgacaat atgaaaatta ttcgaggagt
    10501 gaaaggcaaa aaaacggcct cccgataggg aagccgtagc aaagtgcgcg tgtttttatg
    10561 ccggatgcgg tgtaaacata cgtggcagtg ctatgtaggc atgataagac gcttccgcgt
    10621 cgcatcaggc ataggttgcc ggatgcagtg taaacgcctt atccgtccta cggggtggtg
    10681 ttgttcgtat aaggcgtatc aggcaatagt aattttgcta tcgagataaa cgtcctgcac
    10741 ggcgttaatc agtttcacgc cgtcagccat cgatttcttg aacgccttac gtccaagaat
    10801 cagccccatt ccgccagcgc gtttgttgat aaccgcagta cgcactgcat cactgaggtc
    10861 agtttcaccg cctgcagcac cgccggagtt tatcaaccca gcccggccca tatagcagtt
    10921 agctaactga taacgcacca gatcaatcgg gttttcgctg gttaacttgc tgtacacgcg
    10981 atcgtcggta taaccgtaat taatcgcttt atagccgccg ttattttccg ccattttttg
    11041 tttgacgata tctgcgccga tggttgccgc cagatggttt gcctgaccgg tcaggtcggc
    11101 ggaaacatgg taatcaacgc catctttctt aaaggcggag ttacgcaaat aggcccacag
    11161 cacggtcacc atgcccagct catgcgcacg ttcaaaagcc gcagaaattt cttcaatctg
    11221 gcgacgtgac tcttccgagc caaaatagat agtcgcacca accgccaccg cgcccatgtt
    11281 gaaagcctgc tccacgctgg catacagcgt ttgatcgtag gtattcgggt aacttagcgt
    11341 ctcgttgtga ttaagtttga cgagaaatgg aatgcgatgc gcatagcgcc gcgataccga
    11401 cgccaacacg ccgtaagttg ataccacaca gttacagccc gcttcgatcg ccagttcaac
    11461 aatgtttttc gggtcaaagt agagcgggtt agcagcaaat gaagctccgg cagagtgctc
    11521 aacgccctgg tcaaccggca gaatagaaag atatcctgtg ccagccagac gcccggtgtt
    11581 gtacaacgtc tgcatattac gtaacaccgc tggcgggcga ttattgtcaa tcattacgcg
    11641 gtctacgtag tcatgtccgg ggagataaag ctggtcagaa ggaattgtca tacaacggtg
    11701 ctgtaaaagg ttgtcggcgt ctttgccaag caactgcgca atatctgtca ttactatgct
    11761 cccgtaaatt ccgattggat atcggctatg gattgtcctg gcccgccttt tgcgggcaat
    11821 cataatcctg gtcgttacgg gcgagttttt ccatcttttg gatgtttttt cagcgttttc
    11881 tgttggctcg attcatcaga aaaaatgtta gcgcggtcaa gttttcaccg taaaggcatt
    11941 taaaaggtat tattaagtgg tattgtcatc gcgtacctta cattacctgt catgaaggaa
    12001 ttaaaagatg aaaacaacag caaagctgtc gttcatgatg tttgttgaat ggtttatctg
    12061 gggcgcgtgg tttgtgccat tgtggttgtg gttaagtaaa agcggtttta gtgccggaga
    12121 aattggctgg tcgtatgcct gtaccgccat tgcggcgatc ctgtcgccaa ttctggttgg
    12181 ctccatcact gaccgctttt tctcggcgca aaaagtgctg gcggtattga tgttcgcagg
    12241 cgcgctgctg atgtatttcg ctgcgcaaca gaccactttt gccgggttct ttccgttact
    12301 gctggcttac tctctaacct atatgccgac cattgcgctg actaacagca tcgcttttgc
    12361 caacgtgccg gatgtggagc gtgatttccc gcgcattcgt gtgatgggca ctatcggctg
    12421 gattgcctcc ggtctggcat gtggtttctt gccgcaaata ctggggtatg ccgatatctc
    12481 accgactaac atcccgctgc tgattaccgc cggaagttct gctctgctcg gtgtgtttgc
    12541 gtttttcctg cccggcacgc caccaaaaag caccggcaaa atggatatta aagtcatgct
    12601 cggcctggat gcgctgatcc tgctgcgcga taaaaacttc ctcgtctttt tcttctgttc
    12661 attcctgttt gcgatgccac tggcgttcta ttacatcttt gccaacggtt atctgaccga
    12721 agtcggcatg aaaaatgcca ccggctggat gacgctcggc cagttctctg aaatattctt
    12781 tatgctggca ttaccgtttt tcactaaacg ctttggtatc aaaaaggtat tattgcttgg
    12841 tctggtcacc gctgcgatcc gctatggctt ctttatttac ggtagtgcgg atgaatattt
    12901 cacctacgcg ttactgttcc tcggtatttt gcttcacggc gtaagttacg atttttacta
    12961 cgttaccgct tacatctatg tcgataaaaa agcccccgtg catatgcgta ccgccgcgca
    13021 ggggctgatc acgctctgct gccagggctt cggcagtttg ctcggctatc gtcttggcgg
    13081 tgtgatgatg gaaaagatgt tcgcttatca ggaaccggta aacggactga ctttcaactg
    13141 gtccgggatg tggacttttg gcgcggtgat gattgccatt atcgccgtgc tgttcatgat
    13201 ttttttctgc gaatccgaca acgaaattac ggctatcaag gtcgatgatc gcgatattgc
    13261 gttgacacaa ggggaagtta aatgaaaaca gaacgtattc tcggtgctct ttatgggcag
    13321 gcgttagggg atgcgatggg gatgccctcc gagctttggc cacgcagccg cgttaaagca
    13381 cactttggct ggattgaccg ttttctgcct ggaccaaagg agaataacgc ggcctgttat
    13441 tttaaccgcg ccgaattcac cgacgatacc tcgatggcgc tgtgtctggc ggatgcgttg
    13501 ctggaacgta aaggcaagat cgatccggat ctgattgggc gtaatatcct cgactgggcg
    13561 ctgcgtttcg acgccttcaa caaaaacgtg ctaggtccga cctcgaagat tgcgcttaac
    13621 gccattcgcg acggtaaacc cgttgctgaa ctggaaaaca acggcgtaac caacggcgca
    13681 gcgatgcgcg tctcgccatt aggttgtttg cttccggcgc gcgatgttga ttcctttatt
    13741 gatgatgtgg cgctggcgtc gagcccgacc cataaatccg atctggcagt tgcaggtgcg
    13801 gtagtcatcg catgggcgat ttctcgtgct attgacggag aaagctggtc agcgattgtt
    13861 gattcactgc cttcaattac gcgacatgcc cagcaaaaac gcaccaccac gttcagcgcc
    13921 tcactggcgg cgcggctgga gattgcgctg aaaattgtgc gcaatgccga cggcaccgaa
    13981 tccgccagcg aacagcttta ccaggtcgtt ggcgcaggta ccagcactat tgagtccgtt
    14041 ccgtgcgcca ttgcgctggt tgaactggca caaaccgacc cgaaccgttg cgccgtcctc
    14101 tgcgctaacc ttggcggcga tacagacacc atcggtgcta tggcgacggc aatctgcggc
    14161 gcgttgcatg gcattaacgc tatcgatcct gcgttaaagg cggagctgga tgcggtaaat
    14221 cagcttgatt tcaaccgcta tgccacagcg ctggcgaaat atcgtcaaca acgggaggcg
    14281 gtatgagcgg cgctcgatta cacacgctgc tgcctgaatt aaccacgcgt cagtcggtga
    14341 tggtcgtcgg cgcggcggtc attgacgtga tcgccgacgc ttatgccctc ccctggcgtg
    14401 ggtgcgatat cgaactgaaa cagcagagcg ttaacgttgg cggctgcgca ctgaatattg
    14461 ccgtggcgtt aaagcgcctc ggcatcgaag cgggtaatgc cttgccgctc ggtcaggggg
    14521 tatgggcgga gattattcgc aaccggatgg cgaaagaggg gttaatcagc ctgatcgata
    14581 acgccgaagg cgataacggc tggtgtctgg cgctggttga gccggatggc gaacgtactt
    14641 ttatgtcatt cagcggtgtg gaaaatcagt ggaatcgcca gtggctggcg cgattaaccg
    14701 ttgcgcctgg cagcctgctc tatttttccg gttatcaact ggcctcgccc tgcggcgaat
    14761 tgttagtgga atggctggaa gagctgcaag acgtgacgcc gtttatcgat tttggcccac
    14821 gtattggcga tatcccggac gcattactgg cgcggatcat ggcctgtcga cctttagtgt
    14881 cactcaatcg tcaagaggct gagattgccg ccgaacgttt tgctttatcc gcagagataa
    14941 caacacttgg caagcaatgg caggagaaat ttgccgcgcc gttgatcgtt cgtctcgata
    15001 aagaaggcgc atggtatttc agcaacgacg cttctggctg cattccggca tttccaacgc
    15061 aagttgtcga caccattggg gcgggcgaca gtcatgccgg tggcgtactt gccgggctgg
    15121 cctctggtct gccactggcg gatgccgtat tactgggcaa tgcagtggcg tcatgggttg
    15181 tcgggcatcg gggcggtgat tgtgcgccaa cgcgcgagga actactcctc gcacacaaaa
    15241 acgtatagat cgctgcgaca gtggctaatg ctgtactcaa taggccgttg ctgttggtca
    15301 agcgccactt gcttgatcac cagcaccggt attttgctat ctaactgaat atgtgactga
    15361 aactcggcat ccggcatccg ggcgctaacg cgggaacgcg tacgttgtgg gtaaatgtgc
    15421 tggctgcgga aatagtcata aagtgaaatc ccgatggcat caacatcatg aattaaatgc
    15481 gccggaaccc acgattcctc aatcgatacc gcttcttcat cgacatagcg aatacgctta
    15541 agcaaaaaga catcacttcc tgcttcgacc gccagctgct gcgcgacttc ttcgggacat
    15601 ttcacgacac gcttgttaac ccatagcgta tcgggctttt tcccgcgcaa taccacctgc
    15661 tgagaaaaac ctcgcgcttc tttcaacgag tattcgaaga tgttgttgat ctgcgtgccg
    15721 taaccgcgcg aacgcgtcac cacaccctct tcttccagcg cctgcatcgc cttgcgcacc
    15781 gtaatgcgcg aaacgccggt taactgactt aaatcacgct caccgggcaa aatattgcca
    15841 tgctccagca ccccgctgcg cacggcattt tttaccgttt cggcaaactt aatataaagc
    15901 ggcgtgttat ccgccgctaa aatacgctcg ttcagttggg caatcagctg ggtatgcgct
    15961 tgttccattt gtcttgttcc tgccggggag tctggtggga gtatatcact gcttcttatt
    16021 taaggcgttt ctttaatccc atcaacgaat gcctgcaact cctccaccgt gccattaaat
    16081 acattaaaat ccaccggacc attgatgcca tctacctgtc cacggtcgga atgctgccag
    16141 aagcgccagg ccatgccgtc attgtccgga cgacgttgat aatagtgagc cacccaccac
    16201 ggatattcat tgaaatagcc cgccagattg gtgtgataaa aaacggctcc tgagtaaata
    16261 atcggctttt ttcccgtact tttttcgacc atttttagcc actgacttac tcgtttgcgt
    16321 aattcttttg ccgataattt tccccgttct tctacgtcca gtaccgcagg gaaatcacct
    16381 tgtgagaaat ccaccgtttg cagaaataat cgtgcctgaa ccggcgctgc taccgacggg
    16441 gagaaataat gatacgcccc gcgcagcagg ccattttcgc ggcttagttg ccagttacgc
    16501 gaaaaatacg ggtccaccag cttttcgcct tccgtcgcct taataaaagc aaactgtaag
    16561 cggataccgt tgtcgcgcat ttttgccacc cgctgccagt cgatccgctc ctgccagcga
    16621 gaaacatcta ttccgtgaat ggtgtaactg gcaggaatgc gaatagcgaa agatttaacc
    16681 gggcgataac cgtagaaatg gatgtagtct gtaatctgaa ctgctgtcga atagaaaaaa
    16741 ttcaccttct gacgcggata aattgcaacg atggaaatta gcccaagagc gcataataga
    16801 gaggtgagtt tttttcgact ggtaattctt agctgcattg gtttcatccc tgaatgtcag
    16861 tgccaaaggc tgacaataac caaagcagct atagtacggt gctcaccacc aggcgtggaa
    16921 gtggtgaacc ggaccaatac cgtgaccaac ttccagcgtg tcggcctggg ctaatgctga
    16981 tgaaagccag ctttttgcct cctgtacggt gtcagcccag tttgtatggc gcgggcgtag
    17041 tgcagccaac gccgcagaga gcgtacaacc agtgccgtgg gtgtttttgg tcataatgcg
    17101 cggtgcggta aaccgttgtt caccctcgcg ggtaaacagc cagtccgggc tttgctcatc
    17161 atccagatga ccacctttca ttagcactgc gccacagccc atcgccaaca gcgatcgccc
    17221 ttgttccagc atttcctgtt cagtgcgcgc gtgtggcgcg tcgagcaagg cggcagcttc
    17281 gggcaagttt ggcgttatta atgaaacctg tggcaataat cgactgcgca gcgtagcaac
    17341 cgccgaaggt gaaagcagcg ggtcgccgct ttttgccagc ataacggtgt cgagtaccac
    17401 gtattggatc tgataacgtt gcaaccgttc tgccaccgct tcaacaatat cggtttccgc
    17461 caacataccg attttagtgg tgtcgattcg cacatcgctg aacaccgaat cgagctgggc
    17521 ggcgacaaaa tcagactcaa tgcgatacac cgactgtacg ccacgggtat tttgcgccac
    17581 cagtgcagta ataactgagc aaccataagc gccaagtgcc gagaaggttt taagatcggc
    17641 ctgaatcccc gcaccaccac tcggatcagt accggcaatc gtcagagcgt taattcgttt
    17701 catgcctgca cctcctgcgt caattgccag agcgcatcaa ggaaatgtgg aacaaaactg
    17761 cctggcccct cgcttctggc gactgcgcgt tctccggctt gtttcatcca gtgacaggca
    17821 gatgcgacat tttccagcgt atcgcctggt aacgcacagc aggcagcgac aaccgccgat
    17881 aatgcacagc cagttcctac cactttggtc attaacggat cgccaccgtg aataccaatg
    17941 atacgatgtc catcggtaac ataatccatc tcgccagtga ccacgacgat tgcgccagtt
    18001 tcccgtgcca gtgtttgtgc agcgggtatc gcgttagctg cggcgtcagt ggtatccact
    18061 ccccgtccac cattagcaat gccagctaat gccatgattt ccgaagcatt accacgtata
    18121 gcggttggct tatgaggcag aagttccaga caaaaacggc ggcgataatc gagtgcacca
    18181 accgctactg gatcaagcgt ccagggtgtt tgagagcttt ttgcttgctc aacggcggca
    18241 cacatcgact gtgcgcgtag ctgcgtcagc gtgccaacgt taatcagcaa cgcactagca
    18301 atagccgcaa actgactggc ctcttcggtt tcgataacca tcgctggcga tgcaccgagc
    18361 gccagcaagg tattggcagt aaaggtttgc accacatcgt tggtcatgca gtgcacaaga
    18421 ggggaatgtt ggtgaaaaag gtgtaacgcg tgcgcagatt gcgctgaact ctgcaggtcg
    18481 acttgcatag tttgctcctg ccataacgtg aagaagcaat gacctggtgg tccgtgactt
    18541 ccctacgctg gcattatcca gatcaggtaa tacgggtatt tctcagcctt cacaaagaag
    18601 ggcaccccga gtcatttaaa acaaaatcaa aagattgcga ttaacgtccc gttaatccct
    18661 gttctggatc atgccagcgg aaagccaaag actcaaccct aaaaaatcct ctgtgtccca
    18721 gtttgtgtct cacgttgtgg cccacagata aatggcaggc acaaaaaaac cgcccataag
    18781 gcggcttaag taaattattc agtcgtgaag atttaaatga aatctatttt ctgcatacgc
    18841 tttcttcatg cagtcgaagt gcttttgcca attccaattg gaatgcaaag gaaagacggg
    18901 aacggctgat cttgataccg tcaataatga tgtatgtagg gtaaacagtg accatgcggc
    18961 ccaagtattg gaagtgttta gggtggttta ctttatcgtt attcattgtg tgccatccgt
    19021 ctgggtgggg gtaatcccgg caacggatga ttgatctaaa atcgatcaaa atcaataact
    19081 taacagatca ccaaatccag gggggtgtac attggcgagc ctccctcagc ctaacctata
    19141 cacgaccata aaataggggc ggattccttc cagttgggtg ggaaaatatc tgggagtttg
    19201 gatacagtag ccaggcaggg taaccagacc catagatgag aatgtttatt attcccaaat
    19261 atccaggcga ggggcctgct gtggctctct ttacgcgccg ctgtggcgtc ttctctcctc
    19321 tttctataca atcccatagc taacccattg cgaggcttac agagcgttac agagcgtccg
    19381 tgtgaatatt gcataaatga aagtggtgaa aggtccgatt tggtcaactt ttagacaact
    19441 atttaacata tgcgcaacag gttagacctg taccctttca ataggtccgc ctcctgggcg
    19501 ctgtgtacgc cccataactg tggctcttct gtacccatcc gggagccatt ccacagagta
    19561 aactgtaaaa ctgatctgcc tgtgagtaac gcccttttcc tgtggataac ttccacgcac
    19621 taaccccata aactcccctt ttgaggcgtc cgcctcttca ggccctttct tgtggcaaaa
    19681 acacaaaaag ggtttagggc ggtggctacc gcccctgtag atgggaatga gtcgcactcg
    19741 aaagagggat tgagaaccaa ttctcaagtg ataatcgatc tcatttgtgt tcgaagtttt
    19801 ccccagaaaa ataattttgg ggttaagcat gtaaaatgct acacacttgc actttgtaat
    19861 gataattaat tgaccataaa aacaaatagt tagaagcgct gggggagtcc cactagttac
    19921 acaattgatt gctttttgag ctaaaactga ttcttttacc gggtatacaa tccatttgat
    19981 cgccctttac cacatattta aacggattta aagccattta tttagggggt tagcaaagtg
    20041 ttgattccat ggttacagat aacaaaaaag cccgccgggg gttagccggg ggcctgtggt
    20101 tgggggtgtc aaacgtggtt agttcggccc gccctgcatt agcatgttga aagcctgctg
    20161 attattgctc tcaacttcag agcgtaccag atccttaacc tatccatcct gcactttcac
    20221 attaacgttc acgtttagcg gctggtttag ccacgctggc ggcgtcatca gtggattacc
    20281 tgccatatca actttaagct gtggcgctgt aatcgtcggc gtaggaggta cataagttgg
    20341 attggagtaa gagcttaccc accctgtttt atctgctggt ttggcaaagt ctggattagt
    20401 cggaaggtct ggactatgga ggaaatccca cagtttaccc agcgggctat cttttttcag
    20461 attgcccccg gcggtggtgt cctgtccata catggcgtcg ttagcgtcat gaccttatcc
    20521 caggcataat ccccacctac cgaaccgacc gcaatgctcc agccaacagg accgccagag
    20581 agagccgccg cgccgcctac cttgccaact ttcggacccc gacccggttt gtttggcttg
    20641 tctttacctt ccccaccaga tccgccagta gtagcggctt caatgccacc aatagcggcg
    20701 atccctttca tgacccccag taatttggtt agtgggttaa gcatggtcaa caaccattta
    20761 aaggctacgc ccaacttcca gatagacccg gcgaacatag ccaccccggc ggcgtatgct
    20821 gccatttcgc cccaggcttt aagctgctca ccagtgatcc ccatcttgct ggcgtaatac
    20881 tcaataatgc ggaaggtcag gatcacagtg tcatgcaact ctgtaaacgc atctgtggca
    20941 ccctcaacga ttttcccggc aatatgcccg aacatctcaa aggccccacc agagccgtta
    21001 acagcggcgc tgatagcgtc gaaagcgctg gttaattcgt tgccgaagcc agaagcaaag
    21061 aagatgttca tcgcgttctg catactggtt tgcaggcgct gccagcttgc ccggttggac
    21121 ttcatagcct tatccagagc gccgccgtta cgagctgcgg ctttcatctg tttagccact
    21181 tccgggagga tgtccttaga gaacagcttc ccttgttgca tcaggtcaaa cagatccttt
    21241 tctgtaaggt tggttgtccc tttcatctgg ttgagagcct taacgaacag acccacagcc
    21301 cccgggagag cttcggacaa ctggcctttg agttcttcac tcattaccgt tcctttagac
    21361 atcatctgat tcaaactttt caaagcgcgg ctggcttcat cggctgaagc acctgtaaca
    21421 aggccccatt cactggcacc ctcaaagatg gatcgcatct ggtctttagg gagcttgtca
    21481 ccaacagcgg cggcaaactg agtgtaatca cgggaagtct tgaggagatc cagccccaaa
    21541 cggttggatt gatcacggag ataatcaaac tctttagcgg ccttatctgt ggaaccggtg
    21601 atcgctgtca gaccagaaag cgcggattca aaatcctgtc cggtggtcat gatcttgccg
    21661 c
//
LOCUS       DAJTOR010000058        21567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTOR010000058 DAJTOR010000000
VERSION     DAJTOR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.058"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1080
                     /locus_tag="OO249_001931"
     CDS             <1..1080
                     /locus_tag="OO249_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5636245.1"
                     /translation="TDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGKAEKIAS
                     TANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKNGDIAATTHK
                     EKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLWVDIYKEAQH
                     AGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRR
                     DDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYL
                     IHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSERLYRAQGID
                     TKTLLGHKVQATTDRYNDTRGQEWV"
     gene            1260..2054
                     /gene="potC"
                     /locus_tag="OO249_001932"
     CDS             1260..2054
                     /gene="potC"
                     /locus_tag="OO249_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5636246.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMISPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2051..3097
                     /gene="potD"
                     /locus_tag="OO249_001933"
     CDS             2051..3097
                     /gene="potD"
                     /locus_tag="OO249_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5636247.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETCIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3253..4074)
                     /gene="cobB"
                     /locus_tag="OO249_001934"
     CDS             complement(3253..4074)
                     /gene="cobB"
                     /locus_tag="OO249_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5636248.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     KIQPNAAHLALAKLQEALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4090..5001)
                     /gene="nagK"
                     /locus_tag="OO249_001935"
     CDS             complement(4090..5001)
                     /gene="nagK"
                     /locus_tag="OO249_001935"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5636249.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5030..6274)
                     /gene="lolE"
                     /locus_tag="OO249_001936"
     CDS             complement(5030..6274)
                     /gene="lolE"
                     /locus_tag="OO249_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5636250.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6274..6975)
                     /gene="lolD"
                     /locus_tag="OO249_001937"
     CDS             complement(6274..6975)
                     /gene="lolD"
                     /locus_tag="OO249_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5636251.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(6968..8167)
                     /gene="lolC"
                     /locus_tag="OO249_001938"
     CDS             complement(6968..8167)
                     /gene="lolC"
                     /locus_tag="OO249_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5636252.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8429..9502
                     /locus_tag="OO249_001939"
     CDS             8429..9502
                     /locus_tag="OO249_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5636253.1"
                     /translation="MKQKELWINQIKGLCICPVVIYHSVITFYPHMTTFPHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9630..13076
                     /gene="mfd"
                     /locus_tag="OO249_001940"
     CDS             9630..13076
                     /gene="mfd"
                     /locus_tag="OO249_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5636254.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13220..14182
                     /gene="ldtC"
                     /locus_tag="OO249_001941"
     CDS             13220..14182
                     /gene="ldtC"
                     /locus_tag="OO249_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5636255.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     TRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14265..14522)
                     /gene="bhsA"
                     /locus_tag="OO249_001942"
     CDS             complement(14265..14522)
                     /gene="bhsA"
                     /locus_tag="OO249_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR5636256.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            14763..15395
                     /gene="comR"
                     /locus_tag="OO249_001943"
     CDS             14763..15395
                     /gene="comR"
                     /locus_tag="OO249_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5636257.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15457..15996)
                     /locus_tag="OO249_001944"
     CDS             complement(15457..15996)
                     /locus_tag="OO249_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5636258.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(16223..17527)
                     /gene="ndh"
                     /locus_tag="OO249_001945"
     CDS             complement(16223..17527)
                     /gene="ndh"
                     /locus_tag="OO249_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5636259.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTHDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(17927..18469)
                     /locus_tag="OO249_001946"
     CDS             complement(17927..18469)
                     /locus_tag="OO249_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5636260.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18492..19517)
                     /gene="nagZ"
                     /locus_tag="OO249_001947"
     CDS             complement(18492..19517)
                     /gene="nagZ"
                     /locus_tag="OO249_001947"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5636261.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(19528..20352)
                     /gene="thiK"
                     /locus_tag="OO249_001948"
     CDS             complement(19528..20352)
                     /gene="thiK"
                     /locus_tag="OO249_001948"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5636262.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(20336..20974)
                     /gene="lpoB"
                     /locus_tag="OO249_001949"
     CDS             complement(20336..20974)
                     /gene="lpoB"
                     /locus_tag="OO249_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5636263.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPIVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(20988..21365)
                     /locus_tag="OO249_001950"
     CDS             complement(20988..21365)
                     /locus_tag="OO249_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5636264.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(21368..>21567)
                     /locus_tag="OO249_001951"
     CDS             complement(21368..>21567)
                     /locus_tag="OO249_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HCR5636265.1"
                     /translation="SAEHEQALGRMITVVAKIAEQEGIAEDGYRLIMNTNRHGGQEVY
                     HIHMHLLGGRPLGPMLAHKGL"
BASE COUNT         5337 a         5682 c         5388 g         5160 t
ORIGIN      
        1 actgacgtag ccggtcttta cgaaaagttt gatcgcagaa ctggcagagt ttactaccag
       61 tataaaaatc ctgtgactgg aaaatttcac ggactcggaa cagacaaagg taaggcagaa
      121 aaaatcgctt ccacagccaa tcagcgaata gctgcagcag aagctgaata tttcatgcgc
      181 aaaattgatg aaagtccgtc agcaacaaaa cgtcggggta tcagattaaa ggcatgggtt
      241 gatcgatatc tgaaaataca ggacacgcga ctgaaaaatg gagatattgc agctacaact
      301 cacaaagaaa aaactcgaat ggctgcatac ctggtttccc gtctgggaaa ccacccattg
      361 aaagaactgg aagtaagaga ctttgcatta atactggatg agtggctgga taaagacatg
      421 gtcagcacag cgagagtaaa tcgtggatta tgggttgata tttataaaga agcacagcat
      481 gcaggggaag ttcctcctgg atggaatcct ccggaggcta cccgtaaacc gatccctaaa
      541 gtaaccagag ccaggctcac catggaagac tggcaaaaaa tttacaatgc aacgcctgaa
      601 aaacacttta tccgtaacgc aatgcttctt gcgattgtta ctggtcagcg ccgtgatgac
      661 atttgccaca tgcgtttttc agatgtgtgg aacgaacact tgcatatcac ccagggaaaa
      721 accggaatgc gtctggcgtt accgcttaca ctacgctgtg atgccattgg gataacgtta
      781 aaagaagtta ttgatgggtg ccgagacaga atattaagtc catatctaat ccatagtcgg
      841 caccagaaac aaccgaagcc gatgagtaaa gacaacctga gcgactactt tgccaaagca
      901 cgggatctgg ctgggataat tccaccagca ggaaaaactc cgccaacatt tcatgaacaa
      961 cgctctctat cagaacggct gtaccgtgca cagggtatcg atacaaaaac attactagga
     1021 cataaagtcc aggcaaccac cgatcgctat aacgatactc gaggtcagga atgggtttaa
     1081 gctggttatt tgacaaacac catatggaat atccgtcacc atctcaggaa aaaagtgttg
     1141 taaaatgcgt ggcctagttt tggggaaacg ttttggagag attttggaga aaggaaaaaa
     1201 ttgaataaat tcaaaatcct gaaagtgctc caccttatga ataagaaggt ggaactcgaa
     1261 tgatcggtcg actgcttcgc ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc
     1321 caatcattat tttgattgtg aactccttta acagctcgcg ctttggcatc aactggcagg
     1381 gttttaccac caaatggtat agcctgctga tgaacaacga cagcctgtta caggcagcgc
     1441 agcattcact aacaatggcg gtgttttcgg cgacgtttgc tacgcttatc ggttcactga
     1501 cggcagttgc gctgtaccgt tatcgctttc gcggtaagcc gttcgttagc ggaatgctgt
     1561 ttgtggtaat gatatcacca gatatcgtga tggcaatttc gctgctggtg ctgtttatgc
     1621 tgctgggtat tcagcttggc ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc
     1681 catttgtggt ggtgacggtg tattcgcgcc tgaaaggttt tgacgtgcgg atgctggaag
     1741 cggcgaaaga tctcggtgcc agcgaattta caattctgcg aaaaatcata ctgccactgg
     1801 caatgccagc ggtggcagcg ggctgggtgt taagctttac cctgtcgatg gacgatgtgg
     1861 tagtttcttc gttcgtcacc ggaccaagtt atgaaattct gccgttaaaa atttattcga
     1921 tggtcaaagt cggcgtatcg ccggaagtta acgcgctggc aaccatatta ctggtgctgt
     1981 cgctggtgat ggttattgcc agtcagctta ttgctcgtga taaaacgaaa ggtaacacag
     2041 gggacgttaa atgaaaaaat ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg
     2101 catgagcgcc gctcacgccg atgacaacaa cacgctgtat ttctacaact ggaccgagta
     2161 cgtgccgcca ggactgcttg aacagttcac caaagaaacc tgtattaagg ttatctattc
     2221 gacttacgag tcgaacgaaa ccatgtacgc caagctgaaa acgtacaaag acggtgcgta
     2281 tgacctggtg gttccttcaa cctattacgt cgataaaatg cgtaaagaag ggatgatcca
     2341 gaagatcgac aagtcgaagt taacgaactt cagcaatctc gatccagaca tgctcaacaa
     2401 gccttttgac ccgaataacg actactccat tccgtatatc tggggggcga cggcgattgg
     2461 tgttaacggt gatgcggtgg atccgaaatc tgtcaccagc tgggccgatc tgtggaagcc
     2521 agagtacaaa ggcagcctgc tgttgaccga cgatgcccgt gaagtgttcc agatggcgct
     2581 gcgtaagctg ggctattccg gtaacaccac cgatccgaaa gagattgaag ctgcatataa
     2641 cgagctgaaa aaactgatgc caaacgtcgc agcgtttaac tccgataacc cggctaaccc
     2701 gtacatggaa ggcgaagtta acctcggcat gatctggaac ggttctgctt tcgttgcacg
     2761 ccaggcgggt acgcctattg acgtggtgtg gccgaaagaa ggcggcatat tctggatgga
     2821 cagcctggcg atcccggcaa atgccaaaaa caaagaaggc gcgctgaaat tgatcaactt
     2881 cctgctgcgc ccggatgtgg caaaacaggt tgctgaaact atcggttatc caacgccaaa
     2941 ccttgcggcg cgtaagctgt taagtccaga agtggcgaac gataaaacgc tctacccgga
     3001 tgctgaaacc attaaaaatg gcgaatggca gaatgacgtt ggcgcagcca gcagcattta
     3061 tgaagagtat tatcagaagc tgaaagcagg acgttaattc agcaagcggg cggtggtatt
     3121 accgcccgtt ttttatttag caaggatgtg gctaatgagt caggacagta gagttttatc
     3181 cgactctttc gtcggtattg tggtccgctc gtaggcctga taagcgtagc gcatcaggca
     3241 atgcttcctg ctttataatc ccttcagcaa cttttcaaca aactcaggca ccacctggct
     3301 tgccgggccg taatatttct cggcaaattc attaccgacc tgacttggtt cgagattcag
     3361 ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg ccggataaac
     3421 atgcccggaa gtaccaatgg caatgaaaat atcagccatc gacaacgcca tataaatttc
     3481 atccatgccg agtggcattt cgccaaacca aacgacgtgc gggcgcaagg gtgccgggaa
     3541 ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga gaacctgacc
     3601 actttgcgaa caacgaactt tcagcaattc cccatgcata tgaatgacat tggtattacc
     3661 tgcgcgttca tgcaggttgt ctatattctg cgttaccagc aaaaagcgat cgccgagagc
     3721 ctcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttttg gctgctgcag
     3781 ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat cgaaaccttc
     3841 cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg cgcgaaaggt
     3901 acgaatacct gattccgcag aaattcctgc ccctgtcagt acgagtactc ttggtttttc
     3961 cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac gctcgcgcag
     4021 gcggcgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca gcatagcaac
     4081 ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc ccgcatcacc
     4141 gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac gaggcagcct
     4201 gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa tgacgaccag
     4261 gtcagggtca acaatggtca ggatatttcc cagacaaacc gctaataaat ccagataacg
     4321 ctcaacgtgc gcccttgcct gctcatcgcc ttgattataa agcgcaataa tttcgggagc
     4381 ctgcaactgt tgatgataat agtgttgata cagccacgca aaaccgcgac cagacagata
     4441 attctcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat ccaaccccat
     4501 catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa tgtagctttt
     4561 accggtaatc ggtttgccgt tgaaaatcag cccaccgcca acgccggtac caagaatcaa
     4621 ccccatcacc agtggatatt gcgtaaattc atcatcccag gcttctgaaa gggcaaaaca
     4681 gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt cggcacgcag
     4741 cggtttaccg ctggcagcgg ggacattggc ggcatacagc gtaccatctt ctgtttccgg
     4801 cattcccggt ataccaatgc cgacagaacc tctacagcca aaacgttgat cagcttcggc
     4861 taccagctcg cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg tcggcacccg
     4921 cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt ttgttccacc
     4981 aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt tactggccgc
     5041 taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa cttgccaaaa
     5101 gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc aattccgatg
     5161 gcaggaagtc aataaaatag atatcgctgg agaggaactg atgaccgatc agcttttcaa
     5221 tccgctcaat aatcggggta agttgcagtg aaacaactac gccgataatc agcccacaca
     5281 ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga attaaaccat
     5341 ctttcgcccc cagcgttctt aatactgcga tatcgccact cttatctttc accgccatca
     5401 ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc gccagataca
     5461 taatggcgcg gatcatctgg atatcgcgat acatatagcc gtaagtacca atccagcttt
     5521 taatataaac atagctgttg gtcatttcac ccgcatcgcg taccagctta ttggcgttga
     5581 aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca agatattgtt
     5641 gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttggccactc aactgcaaaa
     5701 tacccgcaac gtgcaaacgc acacgttttg gctgcatcag tttatgctca ggattcgagt
     5761 tggggatcat gatcgacacc caatcgccct gcttcacttt cagcgcatcc gccacgcctt
     5821 tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccacgcatcc ccctgaacaa
     5881 acgagggtaa tgcgctcaga cgctgttcct gttgtgggtt aacgcccttc acctggattg
     5941 cgcgcagatt cgctccactt tccaccagcc cggtgaaatt gatatacggc gcagcggcgg
     6001 caatacccgg cactttctgc actttatcca gtgcttcctg ccagttagtc catggctgat
     6061 ttaccgcttc gatttcacca tgcggcacca ccgccagaat gcggttattc agttcgcgtt
     6121 caaagccgtt catcgcgctt aagccgacga tcaataccgc cacaccgagg gcaatgccaa
     6181 tggtagaaat gacggagatc agcgacacca tgccgccgcg ccgccgtccg cgactaaaac
     6241 gcaggccaat taataacgat aaaggcatcg ccattactcc gcccccatca ggctcagttc
     6301 cgccgtcaga cgtccatcac gcatttctaa ctggcggctc atacgtttcg ccagttgcag
     6361 gtcgtgagta accaccagga aggcggtgcc ctgcaagcga ttcaattccc caagcaactg
     6421 gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggctcatccg ccagtaccag
     6481 acgcggatta ttgaccagcg cacgggcaat cgctacacgc tggcgttcgc cgccagaaag
     6541 ttcagatggg cggtgattcg cacgatgctc cagccccacc gcttttaaca tctccagtgc
     6601 acggctgttg atttcagcgg gctttttctt gccaatcagc agcggcatag ccacgttttc
     6661 cagggcggta aaatccggca gcaggtggtg aaactgataa ataaagccca gtttctggtt
     6721 gcgcagttct gcttttgctg ccgacgacag tttgctcatc ggttgaccgt taaagatcac
     6781 atcgccggag gttggcgtat ccagtccgcc cagcaggtgc agcaaggtgc ttttaccgga
     6841 accagagcta ccgacgatcg ccatcatttc accttcgccg acgctgaaac tgacattgtg
     6901 cagtacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt cgcattgcaa
     6961 caggatctta ttcataacgt aaagcctcag cgggttgtgt ggcggcagcg cgccatgaag
     7021 ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca atgacctgta
     7081 aaggttcgat agccatcggc agcgccgcgc catcaagcag gacgccgatt attggcatca
     7141 gattatttaa ctggctggca agcagtgcgc caagcgccgc tccgaggatc gcaccgataa
     7201 tcccggcgct ggccccttgc accataaaga tcatcatgat ttgtcgcgga gttaagcctt
     7261 gcgtttgcag gatcgctact tcgccctgct tctccatcac catcagcccc agcgaggtaa
     7321 taatgttaaa cgccgcaacg gcgacaatca ggctcagcag taaacccatc atattttttt
     7381 ccatgcgtac ggcctggaac aactcgcctt tacgatcacg ccagtcctgc catttgctgt
     7441 cttcaggcag tttttgctga cttaatgagt cgactttcag cggctcatcc agccacaaac
     7501 gccagccggt aatattgcct gccggataac gcatcagacg cgaggcatcc tcaatattca
     7561 ccagcatttc attgccatcg acttcactgt tggcggcgaa agtgccaatc acattgaaca
     7621 ggcgctgact tggaatacgc cccatcggcg tgaactggct ggcagatggt accatcatgc
     7681 ggatttgatc accgcgatta acgcctagct gtgaggcaag ttgttcgccg aggatgacat
     7741 tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggc gttagtggat
     7801 ctttttgcgc cggatcgata cccagcatca ccccgaccgc cacgctgcgc gcgctttgca
     7861 gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt ttgactgccg
     7921 tttccgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt gcctgtggca
     7981 tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact gacaatactg
     8041 tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag acgaaacgac
     8101 cgaagcgatc cgctgcacgc ccacgcatgt aacgcaggcc aataaataga gcgacaggtt
     8161 ggtacatgaa atccgtctgg ttgctgtagc aaagacacga gtatataagc gaaagcaaga
     8221 tgggtaaacg tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc cccgtaaatc
     8281 aatcggctaa attgccatgt tttatcatca atcttgccgc ctgatcttaa tctgaaaaat
     8341 catccaatac agacagatac ccattacacc tcgggtcata acccgcagga tcaaccgcat
     8401 gtaaattaaa aaagagcgtg ttgtcctgat gaaacaaaaa gagctatgga ttaaccagat
     8461 caaagggtta tgtatttgtc cggtggtgat ttatcactcg gtcattacct tttatccgca
     8521 tatgaccact tttccgcatc cgttatcgga agtcctgagc aaatgctgga tctatttcaa
     8581 tctttacctt gcaccctttc gtatgccggt ttttttcttt atatccggct atttgattcg
     8641 ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacggatct ggaacatctt
     8701 ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg ttaagtgcgc tgaaccagtg
     8761 gctggcacct gagcgcgatt taagcaatgc ctccaatgcc gcttatgccg attccaccgg
     8821 tgagttcctg cacgggatga tcaccgccag caccagcttg tggtatctgt atgcgttaat
     8881 tgtctatttc gtggtatgta aaatttttag ccgtctggcg ctgccactat tcgccttgtt
     8941 tgtactgctg agtgtggcgg ttaatttcgt tcccacgccg tggtggggaa tgaacagtgt
     9001 gatccgcaat ttgccttatt acagccttgg cgcatggttt ggcgcaacaa taatgacctg
     9061 tgttaaagag gtaccgttgc gccgccatct gctgatggct tctttgctta ccgttctggc
     9121 ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg ctggtatcga ttgtggtgat
     9181 catgaagctg ttttatcagt acgagcaacg attcggtatg cgctccacca gcctgctgaa
     9241 tgtgattggc tccaacacca ttgctatcta caccacccat cgcattctgg ttgaaatatt
     9301 cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg tcgccgcaag tcgaactgac
     9361 actcctgctg gtttatccct ttgtcagttt gttcatctgt actgttgcgg gcttgctggt
     9421 aagaaaactt tcacagcgtg cattcagcga tctgttgttc tccccgcctt cactgcccgc
     9481 tgccgtcagt tactcccgct aagccatcgc cccagccggg gcgattcaat ttgctaaacc
     9541 atgtcgatta cggataatga acagcattgc ttatcagaga tatgccccca tatgttgagg
     9601 catatcctaa cgagaatctg acaaccgtta tgcctgaaca atatcgttat acgctgcccg
     9661 tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg cgcagcctgt gcaacgctgg
     9721 tagcggaaat tgccgaacgt cacgccggtc cggtggtact cattgcacca gatatgcaaa
     9781 atgctctgcg tttgcatgat gaaatcagtc agttcaccga tcagatggtg atgaatctgg
     9841 cggactggga aactcttccc tacgacagct tttcgcctca tcaggacatt atctcctcgc
     9901 gcctttccac cctttaccag ctaccgacga tgcagcgtgg cgtactgatt gttccggtga
     9961 atacgcttat gcagcgcgtt tgcccacaca gttttctcca cggtcatgcg ctggtgatga
    10021 aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca actggacagc gccggttatc
    10081 gccatgttga ccaggtgatg gagcacggcg aatacgccac gcgcggcgcg ttgctggatc
    10141 tcttcccgat ggggagtgag ctgccttatc gtcttgattt ctttgatgat gaaatcgaca
    10201 gcctgcgggt gtttgacgtc gacagccagc gcacgctgga ggaagtagaa gcgatcaatc
    10261 tgctgcccgc gcacgaattt ccgaccgata aagcggcaat tgaactgttc cgcagccagt
    10321 ggcgcgatac cttcgaagtg aagcgcgatc cggaacatat ttaccagcaa gtgagtaaag
    10381 gcacattacc tgccgggatc gagtactggc agccattgtt cttcagcgaa ccactgccgc
    10441 cgctgttcag ttatttccct gccaatacct tgctcgtgaa tactggcgat ctggaaacca
    10501 gtgccgaacg tttccaggct gacacgctgg cgcgttttga gaatcgcggc gtcgatccga
    10561 tgcgcccgct gttgccacca caatcgctct ggctgcgggt ggacgagctc ttctcagagc
    10621 tgaaaaactg gccccgggtg cagctaaaaa ctaaacattt accgacaaaa gccgcgaatg
    10681 ccaatttagg tttccagaaa ctgccagacc tagccgttca ggcgcaacaa aaagcgccgc
    10741 tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc ggtggtgttc tcggtagaaa
    10801 gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg aattaaaatt gctccgcaac
    10861 gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt ggcgctgccg
    10921 aacatggttt tgtcgataag gtgcgtaatc tggcgctgat ctgcgaaagc gatctgctcg
    10981 gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac catcaacccc gatacactga
    11041 tccgtaacct tgcggagctg catattggtc agccggtggt ccatctggag cacggtgtcg
    11101 ggcgctatgc cggaatgacc acgctcgaag cgggcggcat tactggcgag tatttgatgc
    11161 tcacctatgc caacgacgcc aaactgtatg ttccggtgtc gtcactgcat ctgattagcc
    11221 gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa acttggcggc gatgcgtggt
    11281 cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa ttgctggata
    11341 tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt gagcagtatc
    11401 agttgttctg cgacagcttc ccgtttgaaa ccacgccgga tcaggcgcag gccattaatg
    11461 cggtacttag cgacatgtgt cagccgctgg caatggatcg tctggtgtgc ggcgatgttg
    11521 gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct ggcagtagat aaccacaagc
    11581 aggtggcggt gttggtgcct accacccttc tcgcgcagca gcattacgac aacttccgcg
    11641 accgtttcgc caactggccg gtacgtatcg aaatgatctc ccgtttccgc agcgccaaag
    11701 agcagacgca aatccttgcg gaagtggcgg aagggaaaat cgatattctg atcggtacgc
    11761 acaaactgct gcaaagtgac gtcaagttta aagatttagg cctgctgatt gtcgatgaag
    11821 aacaccgctt cggggtgcgt cataaagagc gcattaaagc gatgcgcgcg aacgtggata
    11881 ttctgacgct tactgcaacg ccgatcccac gcacgctgaa tatggcaatg agcggaatgc
    11941 gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa acctttgtcc
    12001 gtgagtatga cagcctggtg gtccgggagg cgatcctgcg tgaaattttg cgcggggggc
    12061 aggtttatta tctctacaat gatgtggaaa acatccagaa agctgccgaa cggctggcag
    12121 aactggtgcc agaagcgcgg atcgccatcg gtcacgggca gatgcgcgag cgcgaactgg
    12181 aacgggtgat gaatgatttc catcatcaac gtttcaacgt gctggtttgt acaaccatta
    12241 tcgaaaccgg gatcgacatc ccgacagcca acaccattat cattgaacgc gcggatcact
    12301 tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg acgttcacat catcaggcat
    12361 atgcatggct gctgacgccg catccaaaag cgatgactac cgatgcacaa aaacgtcttg
    12421 aagcgattgc ctcgctggaa gatctcggtg caggctttgc gctggcaacg cacgatctgg
    12481 agatccgcgg cgcgggtgaa ctgcttggcg aagaacaaag tggctcaatg gaaaccatcg
    12541 gtttctcgct gtatatggag ttgctggaaa acgccgtcga tgcactgaaa gccggacgcg
    12601 agccgtcgct ggaagatctc accagccagc aaacagaagt cgagctgcgg atgccgtcgc
    12661 tattgccaga tgatttcatt cctgacgtga atacgcgttt gtcgttctat aaacgtattg
    12721 ccagcgccaa aacggaaaac gaactggaag agatcaaagt cgagcttatc gatcgcttcg
    12781 gcctgctgcc ggatccggcg cgtaccctgc tggatgttgc ccgtctgcgc cagcaagcgc
    12841 agaaactggg gatcaggaag ctggaaggta atgagaaagg cggggtgatc gaatttgccg
    12901 agaagaatca cgttaatccg gcctggttga ttggtttgct gcaaaaacag ccgcagcatt
    12961 accgccttga tggtccgacg cgcctgaaat ttattcagga tttgagtgag cggaaaacgc
    13021 gtatcgaatg ggtacgccag tttatgcgtg aactggaaga gaacgcgatc gcttaatggt
    13081 aaatcggggg cgtttctgcg cccccatcgg caacatttac aaattctttg cacttccctg
    13141 cactatccga cactgtcacc atccataatt caggcttatc tgtttattac aataacctta
    13201 tatttattat ggatttttgg tgatgatcaa aacgcgtttt tctcgctggc taacgttttt
    13261 tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa gccaacacct ggccgctgcc
    13321 gccagcgggc agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa atgacggtgg
    13381 ttctctggaa gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt tacaggctaa
    13441 ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg ttaacgatcc cgttgcaaac
    13501 cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac attgcggagc tgcgtctcta
    13561 ttactacccg ccgggtaaaa attcggtaac cgtgtatcca ataggtattg gtcagttagg
    13621 tggtgacacg ctgacaccga caatggtgac caccgtttca gacaaacgtg caaacccaac
    13681 ctggacgcca acggcaaaca cccgcgcccg ttataaagca cagggaattg agttgcctgc
    13741 ggtagtgccg gctggaccgg ataacccaat gggccatcat gcgattcgtc tggcggccta
    13801 tggcggcgtt tatttgcttc atggtacgaa cgccgatttc ggcattggca tgcgggtaag
    13861 ttctggctgt attcgtctgc gggatgacga tatcaaaaca ctctttagcc aggtcacccc
    13921 aggcaccaaa gtgaatatca tcaacactcc gataaaagtc tctgccgaac caaacggtgc
    13981 gcgtctggtt gaagtacatc agccgctgtc agagaagatt gatgacgatc cgcagctgct
    14041 gccaattacg ctgaatagcg caatgcaatc atttaaagat gcagcacaaa ctgacgctga
    14101 agtgatgcaa catgtgatgg atgtccgttc cgggatgccg gtggatgtcc gccgtcatca
    14161 agtgagccca caaactctgt aaatcctgga taaaaaaaat ccccgcgaca cggcatgtcg
    14221 cggggaaaaa atcagcagta gcaggcatta atgagggtta atgcttattt ataaattact
    14281 gctgttccat ggagggtatt cggaccggtt acagaagtaa tacggaaaga ttttgcaccc
    14341 atctcatccg ctttttgcgc cagctgctct tccagcgatc ccagatttgt ccccgcttta
    14401 gcactgattg taccgacttt ttgttggcct tctggcgttg actgaacttc gacagccgca
    14461 aagctggcaa atgacatgga gcttaaaatc gccgcagcga tgagggtttt tacgtttttc
    14521 ataatagtgg ccttatgcag atgaatgacg tcgcaagcaa ttacttaacg atcgataact
    14581 aaatgacaga tgtgatctgg atcacataca agatattttt tataacaatc attaattaat
    14641 ttaaaaatct ttaaatttca gttaaataga aagcaattat ttttatggga tctggactgg
    14701 tgaacggagg tgattatttt tataataatc tttcattcac agtcaccagg tacaacggca
    14761 tcatggcaac tgactcaaca caatgtgtaa aaaaaagccg tggccgccca aaagtgttcg
    14821 acagggatgc cgcgcttgat aaggccatga aattgttctg gcaacacggt tatgaagcga
    14881 cttctcttgc ggacctcgtc gaagcgaccg gagccaaagc gcccacgcta tacgcggaat
    14941 ttaccaacaa agaggggtta tttcgtgccg ttctcgaccg ctatatcgat cgttttgccg
    15001 ctaagcatga agcacagctg ttttgtgaag agaaaagcgt ggagtctgcg ctggctgact
    15061 attttgctgc catcgccaac tgctttacca gcaaagacac cccggcaggc tgcttcatga
    15121 tcaacaactg caccaccctc tccccagatt caggagatat cgccaatacg ttgaaatcac
    15181 gccatgcgat gcaagagcgc actttgcagc agtttttatg tcaacgacaa gcgcgcgggg
    15241 aaatcccgac ccactgtgac gtgacacatc tggcagaatt ccttaattgt attattcagg
    15301 ggatgtcgat cagcgcacgc gaaggtgcat cgctggaaaa actaatgcag attgccggaa
    15361 cgactttgcg tttatggccc gaactggtga aataagtcgc aaaaaaagcc cctcagcctg
    15421 agcgaatgag gggccaaatt gcagagtaca gccttgttat actttgttat tcaaaatcag
    15481 ttgcccatta ctatctagcg ggatctgcgt acccggatcg cggtccatgc ggattttgcc
    15541 ctgctgatcg ccaatcttat aggtcacatc ataaccgagc attttttctg acttgtcata
    15601 caccgtttta caacgctgtt gcgtagtcgt gtaagtatcg ctttcctgga gagagccctg
    15661 gatctggtta ccggcatatc caccacccag cgcccccaca acagtggcga catctttacc
    15721 gcgaccaccg ccaaactgat gccctatcac gccgccagca acagcgccga gcaccgaccc
    15781 ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc actgtgacgt tgcgacactc
    15841 ctgacgcggt gttttaaccg tttccttgat tggggttgca gaaacaacct gagcgtattg
    15901 cgggccacgt tcaaacacgt tcagactggc cactgccgct acgcccagcg cagctgcgac
    15961 accaatcccg atacccgcca acattgattt attcacggga acacctcctt ctttgcagtt
    16021 atgccaatca tgcaagcgaa ggagaaaagc agctatcaga cagaagatga aaagcgcggg
    16081 aatggctgga aatacttaat gattcggagg agtctgaagg aatgcctgat gcgacgctta
    16141 acgcgtctta tcaggcctac cgctcgtacc atttgtccag ggcggatgag gcgtttatgc
    16201 cacatccgcc agtgtacgtc gattaatgca acttcaaacg cggacggata acgcggttaa
    16261 tactccccac cagcatcatt aatccggttt taaagtaacc atgcagcgca atctgatgca
    16321 ttcggtatag cgagatatat acaaagcgcg caattcgtcc ttcaatcatc attgagccgc
    16381 gcgtcaagtt acccatcagg ctaccaacgg tggagaagtt cgacagcgat accagtgaac
    16441 catgatcttt atactgataa tttttcagcg gcttaccgtt tatctgcgcc agaatattgt
    16501 tcattgcgca agtcgccatc tggtgtgcag cctgagcacg cggtggaaca aagccccctt
    16561 ccggacgcgt gcatgacgcg cagtcgccaa tagcgtaaat gtctggatcg tgggtggttt
    16621 gcagcgtcgg ttccaccacc agctggttga tacggttagt ttcaagacca ccgatatctt
    16681 tcaggaagtc tggcgctttg atcccggctg cccacaccat cagatcagcc tcaatatatt
    16741 caccatcttt agtgtgcagg ccgccttcat cagcactggt gaccatggtt tgcgtcagca
    16801 cgcgaacgcc aagtttcgtt aactcactat gggccgcagc agagatgcgt ggcggtaatg
    16861 caggcaaaat acgttctccc gcttctacta gcgttacgtt cagggcttcg ttggtcagac
    16921 ctttataacc gtagctgtgc agttgcttga ccgcgttatg caactcagcg gagagttcta
    16981 cacccgtcgc gccgccaccg acaatcgcaa tgttcacttt gccattcgcg cccaggttgg
    17041 cggagtattt caggaacaga ttcagcatct cctggtggaa gcgacgcgcc tggtgcgggt
    17101 tatcgaggaa aatgcagttc tctttgacgc ctggcgtatt gaaatcgtta gaggtgctac
    17161 ccagcgccat gaccagggtg tcataggcga ttttacgttc cggaaccagc agttcacctt
    17221 tctcgtcgcg tagttctgca atagtgattg ttttcgcttc acgatcaata tcaatgacgg
    17281 aacccaactg gaactggaaa ccatgattgc gcgcatgggc cagatagctc aacgcatcga
    17341 cgccttcatc aagcgagcca gtcgccactt cgtgcagcag cggtttccac aggtggctgt
    17401 ggttacgatc gaccagcgta attttggctt ttttcttacg tcccagctta tgccccagct
    17461 gtgttgccat ttccagccca ccagcaccgc cgccgacaat cacaatcttt ttcaatggcg
    17521 tagtcaacgt gacccccttg aatttattaa ccaattgtta attaatggtt attaacatag
    17581 ctcttaatta acaacaggtt acagcaatga aaatgttctt cgctattgag aataacatga
    17641 atggtgcatt ggtcatacca aaattgatgt taatcaagtt ttgttgctga aaagataggc
    17701 gactttaccg ggtgagatca aaaaatagag tttattgttt attatttgag cgaatttgtg
    17761 agagtgatat agagtgaggg gtgtatgcag ggcgttcggt aggcctgata agaggcgcat
    17821 caggcattga tgctcgactg tcggatgcgg cgtaaacgcc ttatccgact tacagaattc
    17881 ggagcgcatc aggtaccggc aaggttacct gatgcgacga gggcatttac ccgagggttt
    17941 tgaacgcttt aatgcgctgc aaatgcgggg agatattctt gaatttgtgc gtctgctctt
    18001 cgtcccagac aatctcgtaa taatgatgca actcttcaga tgtccgctgg ctgttaagcg
    18061 cttcatcatt acgcgacaaa atcaccaggc aacgatcgcg attcttctcg cggaagttgg
    18121 tcacacactt agtggcaata tcggcatact cttccgggcg atcaatcttg ccttccatgt
    18181 tctcataagg gaacaaatta gggttgaaga tcacctggcg gatatcgcaa agaaaaccaa
    18241 tccgttccgc ccagtatccg cccagaccaa cgccgcaaat cagcggacgc tcgtcgacgt
    18301 tcagttgcaa cattttgtcc acttctttaa gcagatgctg catatcatgt ttcggatgcc
    18361 gcgtactgta gcttatcaag cgtacatccg ggtcaataaa ctgcaattgt aagactttct
    18421 cgtggttacc cggactgtta gagtcaaaac cgtgtaaata gataatcatc gcttcctcac
    18481 ataagccagg gttagtgacc tgctttctct tcctgccagc gttcatgtaa ctgattcaga
    18541 cgggtgctgc tcgccttcca gcgagccgag tccatcagtt cctgtcgcga aaatgaacct
    18601 ttatgataca aacgtgtaac acgttctgcc ttgatcggtg acagattatc taacacgctg
    18661 acggcccctt tacgattatt gcagaccagg atcatatcgc aacctgcgtc cagtgatgcc
    18721 tgcccgcgtt cggcataact gcccataatc gcggcacctt ccatcgataa atcgtcagag
    18781 aaaattacac cgtcaaaaca cagttcctga cgcaaaacgg ttttcagcca gtaagaagaa
    18841 ccgctcgccg gacgcggatc aacatcactg tagatcacat gcgcaggcat aatggcgtcg
    18901 agtttatttt cgcggattaa cgtgctgaaa accgacatat ctttggcacg aatttccgct
    18961 tgcgggcgtg ggtcgcacgg cgtctctttg tgtgaatctg cagttactgc accgtgtcct
    19021 gggaagtgtt tcccggtcgt tttcattccg gcttcatgca tcccatcaat aaaccgacta
    19081 gcgattgcca gggctttttc tgggtcggca tgataagaac gctcgccaat cgccgcgctg
    19141 atatgtccta catccagcac tggcgcaaag ctgatatcaa tatccatagc gatcatttcg
    19201 ctggccatca gccaacccgc ctcttgcgcc agtttgccgc cctcttccat tcccaacagc
    19261 gcagcaaagg attgtgctgc cggtaagcgg gtaaaacctt cgcgaaaacg ctgcacgcgt
    19321 ccaccttcct gatctaccgc caccaccaga tgattgcgcg atgctgcgcg gatctggcgc
    19381 accagttcac gtaactgggc aggatcatga tagttacgcg taaagagaat cagccctccc
    19441 accagcggat gcgccagtat ttcacgctct tccgcgtcca gttcataacc ttcgacatcc
    19501 aacattactg gacccacact gctctcctta ttgttttatt aatagctgcc gccaggtgtc
    19561 atcggccagc ctgataaatt gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc
    19621 tgctttgagc atcagcagcc agggaaacca tcgcctgacc tgacgccata attgcgccgg
    19681 ataaatcttc gcgcgagtgg catagtcatt gaccaattgc cggtgctgtt cagtattttc
    19741 cacccacacc gccgccagtt ccagcgcggt atcaccatct ccggcatact cccagtcgat
    19801 gagttttaac cctgatgcgc tatgcactaa atttccggcg tggacatcca tatgcaatgg
    19861 acttaagcgt aaaggtcgtg gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca
    19921 acccacagtc cgccgcgccg gatcgctttg ctgccagtac agttccagta acggcaacag
    19981 cgttattcgc cagccaaaac gtggttgttg atgtagataa tacagcaagc ctgccagttc
    20041 gttggtatcc ggcaaatacg tttttaccgc gccgggcaga tagtcgacta ccatccagtc
    20101 acggagatat aaatgcggct tcggtgcaat gcttgcgggt agttgtgata aagcccgata
    20161 ctggcgcaag aacgcggact gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg
    20221 ctggccctga tgttcaatga gaaaactacc gccactaagc ccactattaa acgtcgtgac
    20281 gggatgaaac tgcgggaaaa agcgcgacag caattcgtcg cgcgtgatgg gattattact
    20341 gcgaaacggc acctttacct gaccagataa tttcgcccgt ctgcaccagc atcagctgca
    20401 tttgtagggt cggagcgtta acgttgccag aggcgctgga gtacagcacg taatgagcgc
    20461 cgacattgcg ggcaatgcct atggctttac tacgggtgcc taaactgtcc tgcggcgaca
    20521 aacctaactg ttgcttagcc atcgacagtt gctgggcgga aaccagggta aatttcccgt
    20581 tattagccag cgcatttcgc agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag
    20641 tacggttgtt aacgctatca accagcagga cgctacccgc agtcaccccg tcagccccaa
    20701 gcatcttact gactatcggc tgcattgcgc cattccagtc ataatggcga atatgcggcg
    20761 caggcggtgc cgttcgatct tcgtgctcaa ttgggcctgg ctgctgcggg atcgtcggca
    20821 ccgagggcac tgtggggaca ggctgttgtg gctcggctgg ttgttccggc gctggtttca
    20881 cttcttctac cggtgcaggt tcacgttgcc ccacacaccc ggcgagaaac atcgccagcg
    20941 cggtaatcaa ggcgtagcga ctcatttttg tcatcaagat tcacccctta caaatataga
    21001 taaagtctga ctttgtgcgc ccccagaaaa ttggcgctgc cgtacagcgt taccgccgaa
    21061 tgtgcgggaa tggtaacgct gcgtggcctt tccagaggat gcatctccag ccctctggcg
    21121 tcataccagt aaaaacgata atgaacggta acgggttttt gcctttcgtt ataaagcgtt
    21181 gaggatgctg aaggttgaat atcagacgtc gaaaggacgg gcttttctgc actgatgccc
    21241 gcagccaata aagatgactc catcaccagc gattgctcat cattcaccgg aatttccgga
    21301 tgtgaacgac agcccaccag cagtaacaac accagagaca ccagcccaaa gcatcctttt
    21361 ctcatcgtta cagaccttta tgcgccagca ttggtcccag cggacggcca cccaacaagt
    21421 gcatatggat gtggtaaacc tcttgtccgc catggcggtt ggtattcatg atcagacgat
    21481 agccatcttc ggcaatacct tcttgctcag caatttttgc cactacggtg atcatgcgtc
    21541 ccagcgcctg ctcatgctca gctgaga
//
LOCUS       DAJTOR010000059        21361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTOR010000059 DAJTOR010000000
VERSION     DAJTOR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.059"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..217
                     /locus_tag="OO249_001952"
     CDS             <1..217
                     /locus_tag="OO249_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5636266.1"
                     /translation="EIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADAN
                     RNMTEGGRDLMKSVGKAEENKSDSWFN"
     gene            complement(312..1889)
                     /gene="guaA"
                     /locus_tag="OO249_001953"
     CDS             complement(312..1889)
                     /gene="guaA"
                     /locus_tag="OO249_001953"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5636267.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYNKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(1958..3424)
                     /gene="guaB"
                     /locus_tag="OO249_001954"
     CDS             complement(1958..3424)
                     /gene="guaB"
                     /locus_tag="OO249_001954"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5636268.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            3586..4956
                     /gene="xseA"
                     /locus_tag="OO249_001955"
     CDS             3586..4956
                     /gene="xseA"
                     /locus_tag="OO249_001955"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5636269.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(4953..5168)
                     /locus_tag="OO249_001956"
     CDS             complement(4953..5168)
                     /locus_tag="OO249_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5636270.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5237..6709)
                     /gene="der"
                     /locus_tag="OO249_001957"
     CDS             complement(5237..6709)
                     /gene="der"
                     /locus_tag="OO249_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5636271.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEVEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(6827..8005)
                     /gene="bamB"
                     /locus_tag="OO249_001958"
     CDS             complement(6827..8005)
                     /gene="bamB"
                     /locus_tag="OO249_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5636272.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8016..8636)
                     /gene="yfgM"
                     /locus_tag="OO249_001959"
     CDS             complement(8016..8636)
                     /gene="yfgM"
                     /locus_tag="OO249_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5636273.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(8654..9928)
                     /gene="hisS"
                     /locus_tag="OO249_001960"
     CDS             complement(8654..9928)
                     /gene="hisS"
                     /locus_tag="OO249_001960"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5636274.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDLNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10039..11157)
                     /gene="ispG"
                     /locus_tag="OO249_001961"
                     /gene_synonym="gcpE"
     CDS             complement(10039..11157)
                     /gene="ispG"
                     /locus_tag="OO249_001961"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5636275.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11184..12197)
                     /gene="rodZ"
                     /locus_tag="OO249_001962"
     CDS             complement(11184..12197)
                     /gene="rodZ"
                     /locus_tag="OO249_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5636276.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLLGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(12482..13636)
                     /gene="trmG/rlmN"
                     /locus_tag="OO249_001963"
     CDS             complement(12482..13636)
                     /gene="trmG/rlmN"
                     /locus_tag="OO249_001963"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5636277.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(13786..14217)
                     /gene="ndk"
                     /locus_tag="OO249_001964"
     CDS             complement(13786..14217)
                     /gene="ndk"
                     /locus_tag="OO249_001964"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5636278.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(14366..16678)
                     /gene="pbpC"
                     /locus_tag="OO249_001965"
     CDS             complement(14366..16678)
                     /gene="pbpC"
                     /locus_tag="OO249_001965"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5636279.1"
                     /translation="MLRLLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLEWPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHYAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(16679..>21361)
                     /gene="yfhM"
                     /locus_tag="OO249_001966"
     CDS             complement(16679..>21361)
                     /gene="yfhM"
                     /locus_tag="OO249_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCR5636280.1"
                     /translation="DQDFSRVIHVVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIV
                     TIGKEVKALNNATFSKDYEKTITTRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNV
                     DVNFFRVKPESLPAFISQWEYRNSLANWQSDKLLQMADLVYTGRFDLNPARNTREKLL
                     LPLGDIKPLQQAGVYLAVMNQAGRYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLE
                     NGAAQQGIEVSLLNEKGQTLTQATSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLP
                     ALDLAEFNIAGAPGYSKQFFMFGPRDLYRPGETVILNGLLRDADGKALPDQPIKLDVI
                     KPDGQVLRSVVSQPENGLYHFTWPLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPE
                     RMALNLTGEKTPLTPNDEVKFSVVGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFE
                     FGDIAAENLSRTLDEVQLTLDDKGRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVT
                     RRAEQAIWPANALPGLRPQFALKSVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVK
                     KAVSGLQVRLIRERRDYYWNWSEDEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVE
                     WGAYRLEVKAPNEAVSSVRFWAGYSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKL
                     HIAAPTAGKGYAMVESSEGPLWWQEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPS
                     DKSRSATPKRAVGVLHLPLGDENRRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQ
                     VNVLVSAVDSGVLNITDYVTPDPWQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGG
                     DGDELKRGGKPLVNHVNIVAQQALPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADD
                     FGSNESKVIVAAPVIAELNMPRFMASGDTSRLTLDITNLTDKPQKLNVALTASGLLEL
                     VSDSPAAVELAPGVRTTLFIPVRALPGYGDGEIQATISGLALPGETVADQHKQWKIGV
                     RPAFPAQTVNYGTALQPGETWAIPADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELK
                     AYPYGCLEQTASGLFPSLYTNAAQLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGG
                     FALWDKNGDEEYWLTAYVMDFLVRAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPY
                     ADNLKASKFAVQSYAALVLARQQKAPLGALREIWEHRADAASGLPLLQLGIALKTMGD
                     ATRGEEAIVLALKTPRNSDERIWLGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTL
                     SQQAFGERWLSTQESNALFLAARTIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQL
                     ATLQVRNSGDQPLWLRMDASGYPQSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDL
                     VLVWLQVKASNSVPDALVVDLLPAGLELENQNLANGSASLEQSGDEVQNLLNQMQQAS
                     IKHIEFRDDRFVAAVAVDEYQPVTLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAA
                     EDLLIVRP"
BASE COUNT         5050 a         6019 c         5290 g         5002 t
ORIGIN      
        1 agagattcag aaacaaaaac tggcgctggc aaaagaagaa gcccgcgtta agcgagaaaa
       61 cgaatttatc gatcaggaac tgaagcacaa agctgcgcaa accgatgtgg tgcaatctga
      121 agctgacgcc aacagaaata tgactgaagg cggtcgcgat ctgatgaaaa gcgtgggcaa
      181 agcagaagag aacaaatcgg acagctggtt taattaatcg atgttagtaa cttcaagcct
      241 atgattcttg aagataaaaa accctctgta gtaacagagg gttttgttca ttcatagtgc
      301 agggattaaa atcattccca ctcaatggta gctggcggct tgccgctgat gtcatacacc
      361 acgcgggaaa taccgttcac ttcattgata atgcggttgg aaacgcgacc gaggaaatcg
      421 tacggcagat gcgcccagtg tgcggtcata aagtcgatgg tttcgacagc acgcagagag
      481 acaacccagt catacttacg accatcgccc attacgccaa cggaacgtac cggcaggaac
      541 acagtgaacg cctggctgac tttgttgtac aggtccgctt tacgcagttc ttcaatgaag
      601 atggcgtcag cacggcgcag caggtcacag tactctttct tcacttcacc cagcacacga
      661 acgccaaggc ctggtcccgg gaacgggtga cggtacagca tgtcgtacgg caggcccagc
      721 tccagaccaa tcttacgcac ttcgtctttg aacagctctt tcagcggttc aaccaggccc
      781 atcttcatct ctttcggcag gccgcccacg ttgtggtgag atttgatgac gtgtgcttta
      841 ccggttgcag aagccgcaga ttcgataacg tcagggtaga tggtgccctg cgccagccac
      901 ttcacgtctt ccagtttcag cgcttcttca tcgaacactt caacgaaaac gcgcccgatg
      961 attttacgtt ttgcttccgg atcgttttcg ccagccagcg ctgacaggaa gcgatcttct
     1021 gccggtacgt gaacaatgtt cagaccaaag tgatcgccaa acatatccag aacctgctct
     1081 gcttcgttga gacgcagcag gccgttgtcg acgaatacgc aagtcagatt tttaccgata
     1141 gcgcggtgca gcagcattgc ggttacggag gaatccacac caccagagag gccgaggatg
     1201 actttatcgt cgcctacctg ctcgcggatg cgagctacag catcgtcgat aattttcgct
     1261 ggcgtccaca gggcttcaca ctggcagata tcacgcacaa aacgctccag catgtgcata
     1321 ccctggcggg tgtgagtcac ttccgggtgg aactgtacgc catagaagcg tttttcttcg
     1381 ttagccataa tggcaaacgg gcagctttcg gtgctggcta cggtgacgaa gtcggacggg
     1441 atagcggtaa ctttatcgcc gtggctcatc cagacatcga gcagcggttt accgtctgcg
     1501 gtcagcgcat cttcgatacc gcgaaccagt gcgctgtcgt ttacgacttc aacctgcgcg
     1561 tagccaaatt cacgttcgtt agaggcttca acgtgaccgc ccaactgcat tgccatggtc
     1621 tgcatgccat agcaaacgcc gaataccggt acgcctgctt caaagacata ctgcggcgca
     1681 cgcggactgt tttcttcagt agtactttcc gggccgccgg aaagaataat gccgcttgga
     1741 ttgaagtcac gaatttgtgc ttctgtcaca tcccacgccc acagttcgca gtaaacaccc
     1801 agctcacgca cgcggcgcgc aaccagttga gtgtactgag aaccgaagtc cagaatgagg
     1861 atgcgatgct tatgaatgtt ttccgtcatt gacgcttatt ccgaggcaag tgaaacagat
     1921 aatataaatc gcccgacatg aagtcgggcg aagagaatca ggagcccaga cggtagttcg
     1981 gggactcttt agtaatggtc acgtcgtgaa cgtggctttc ctgaatgccc gcaccgctga
     2041 tacgtacaaa ctccgcttta gtacgcagtt cgtcgatagt accacagccg gtcaggccca
     2101 tacaggagcg caggccgccc atctgctggt gaatgatctc tttcaggcga cctttatagg
     2161 ctacgcgacc ttcgatacct tccggcacca gtttgtcggc agcgttgtca ctctggaagt
     2221 aacggtcaga agaacctttg gacatcgcac ccagagagcc cataccacgg taagatttgt
     2281 aagaacggcc ctggtagagt tcaatttcac ccggagattc ttcagtaccc gccagcatgg
     2341 aacccaccat taccgcgctt gcgccagcgg cgatagcttt ggcgatgtcg ccggagaagc
     2401 gaataccacc atctgcgata accggaatac ctgtgccttc cagcgcttca actgcgtcag
     2461 caacggcagt gatctgcgga acacctacgc cagtaacgat acgagtcgta cagatagaac
     2521 cagggccgat accgacttta accgcgctgc aaccagcttc tgccaaagcg cgtgcacctg
     2581 cagctgttgc cacgttgccg ccgatgattt gcagatccgg atatttagca cgggtttcac
     2641 ggatacgctg caaaacgcct tctgagtggc cgtgggagga gtcgatcagc agaacgtcaa
     2701 cgccagcggc aaccagcgcg tcaacacgct cttcgttacc cgcacctgcg ccaactgcag
     2761 caccaacacg cagacggcct tgctcgtctt tacaggcgtt cggtttacgt tccgctttct
     2821 ggaagtcttt cacggtgatc atgccgatca ggtggaattc gtcatcaacc accagcgctt
     2881 tttcaacgcg tttttcgtgc atttttgcca gcaccacttc acgggcttca ccttcacgca
     2941 cggtgaccag acgctctttc ggcgtcatgt aaacgctaac cggctggttc aggtcggtaa
     3001 caaaacgcac gtcacgaccg gtgataatac ccaccagttc gttttcttcg gtaacaaccg
     3061 gatagcccgc aaaaccgtta cgctcggtca gttctttcac ttcgcgcagc gtcgtggttg
     3121 gcagaacagt ctgcggatca gtcaccacac cagattcgtg ttttttcaca cggcgaactt
     3181 cttctgcctg gcgttcaatg gacatgtttt tgtggataaa gccgataccg ccttcctgag
     3241 ccagagcaat agccaggcgc gcttccgtta cggtatccat tgctgcggaa agcataggga
     3301 tattcagacg aatagttttc gtcagctggg tgctgaggtc agcagtattc ggcagaacgg
     3361 tagaatgagc aggaacgagg agaacgtcgt caaacgtcag agcttcttta gcgatacgta
     3421 gcatgggcaa tatctcgacc agagtggtta ataaatattg ccgcggcatt atacagagcg
     3481 taaccgattg catctacccc tttttgcaaa aaatgcttgc tatccccgaa gggcgggtta
     3541 ctatcgactg aataacctgc tgatttagaa tttgatctcg ctcacatgtt accttctcaa
     3601 tcccctgcaa tttttaccgt tagtcgcctg aatcaaacgg ttcgtctgct gcttgagcat
     3661 gagatgggac aggtttggat cagcggcgaa atctctaatt tcacacaacc ggcttccggt
     3721 cactggtact ttacactcaa agacgacacc gcccaggtac gctgcgcgat gttccgcaac
     3781 agcaaccgcc gggtgacctt ccgtccacag catggacaac aagttttagt tcgcgccaat
     3841 attacgctct acgagccgcg cggtgactac cagataatcg ttgagagtat gcagccggcc
     3901 ggtgaagggc tgctgcaaca gaagtacgaa cagctcaaag cgaagttgca ggctgaaggt
     3961 ttgttcgatc agcaatacaa aaaaccactt ccctcccctg cgcattgcgt tggtgtgatc
     4021 acctcaaaaa ccggtgctgc gctacatgat attttgcatg tgttaaaacg tcgcgatcct
     4081 tctctaccgg tgatcatcta ccccaccgcc gttcagggcg atgacgcacc ggggcaaatt
     4141 gttcgcgcca ttgaactggc gaatcagcgc aatgagtgcg acgtgttgat cgttgggcgc
     4201 ggcggcggtt cgctggaaga tttatggagt tttaacgacg aacgcgtagc gcgggcgatt
     4261 tttgccagcc gcattccggt cgtcagcgcc gtcgggcatg agacggatgt gaccattgcc
     4321 gattttgttg ccgatctgcg tgcgccaacg ccgtctgccg ccgctgaagt agtgagccgt
     4381 aatcagcaag agttactgcg ccaggtgcaa tcgacccgtc aacggctgga gatggcgatg
     4441 gattattatc tcgccaaccg cacgcgtcgc tttacgcaga tccatcaccg attacagcaa
     4501 cagcatccgc agctccggct ggcacgccag caaaccatgc ttgagcgcct gcaaaagcgg
     4561 atgagctttg cgctggaaaa tcaacttaag cgtaccgggc aacagcagca gcggttaaca
     4621 cagcggctga atcagcaaaa tccacagccg aagattcatc gcgcgcaaac gcgcattcag
     4681 caactggaat atcgtttagc agaaaccctg cgcgtacagc ttagcgccac gcgtgaacgt
     4741 ttcggtaatg cagtaacgca cctcgaagcc gtaagcccac tgtcaacgct ggcgcgtgga
     4801 tacagcgtta ctactgctac tgacggcaat gtactgaaaa aagtgaagca agttaaagcg
     4861 ggtgaaatgc taaccacacg tctggaagac ggctggatag aaagtgaagt taaaaacatc
     4921 cagccagtaa aaaaatcgcg taaaaaggtg cattaagcca gcacgaattc aacgcgtttt
     4981 ttcgatatca agccatgccc gtgctgacag aagtaatcta ctgcaccgca ggcttttaac
     5041 acctgcaacg gttgatggca gtctggacaa agagctttca tttcaataat ttccccgcag
     5101 gatgggcaac gcgcatggcc gttatcctga tcaagcacat gctgacattg tggacaatgc
     5161 agttccatat gaattcctcc ggtagccatc cctctacatt catagaggga atggcagata
     5221 aaatacttac ggataattat ttatttttct tgatgtgctt catcagacgc ttacgtttac
     5281 gcatctgggt tggcgtcagg gtgttacgct tgttcgcata cgggttttcc ccttctttga
     5341 actgaatacg aatcggcgat cccattacgt ccagcgattt gcggaagtag ttcatcaagt
     5401 agcgcttgta ggaatcaggc aggtctttca cctgattacc gtgaatcacc acaatcggcg
     5461 ggttataacc accagcgtgg gcatatttca gcttcacacg acgaccgcgt accagcggcg
     5521 gttggtgatc ttcaacagcc atcgtcatga tgcgcgtcag catagaggtc cccacacgac
     5581 gggtggagct gtcatacgct tcacgtactg attcaaacaa gttaccaaca ccactgccgt
     5641 gcaaggcaga gataaagtgc acacgagcaa aatcgataaa gcccagacgg aagtccagcg
     5701 tttctttcac ctgctctttc acttcctggc tcaggccatc ccacttattc accacaatga
     5761 caagtgagcg cccactattg agaataaagc ccagcagcga gagatcctga tcggaaatac
     5821 cttcgcgcgc atcaatcact aacatcacca cgttggcatc ttcaatggcc tgcaacgttt
     5881 tgattacgga gaatttctct acagcatcgg tgattttgcc gcgtttacgt acgccagcgg
     5941 tgtcaatgag cacatactca cgtccatcgc gttccattgg aatgtagata ctgtcacgcg
     6001 tcgtgccagg catgtcataa acaacaacgc gctcttcacc aagaatacgg ttagtgagtg
     6061 tagacttacc tacgttcgga cgacccacaa tcgccagttt gatcggcaga ctttgcgggt
     6121 cgaagtcgtc ttcctcttct tcttcgccgt tctcttccac ttcaaattgc gcccagtatt
     6181 cagcgtcttc gtcgacttcc tcttgcggtg cgagatcttc catccacggc agcagcacat
     6241 gctccagcag acttaatacg ccacgaccgt gagacgcggc gatcgggtag atttcaccta
     6301 agccaagcga gtagaaatca accactgcct gatcgggatc cagaccgtca gttttgtttg
     6361 ccaccaggaa ggtcggtttt tcacgggagc gcagatgttt ggcaatcgct tcatctgccg
     6421 gcatcaggcc cgcgcgcgca tccaccataa acagtacgac gtccgcttct tcaatcgcca
     6481 gcagcgactg ttccgccatg cgggtttcta caccgtcttc tgtgccatca atcccgccgg
     6541 tatcaataca gataaactca cggccttcaa tttccgcacg accgtactta cggtcacgag
     6601 tcagacccgg gaaatccgca accagcgcat ctcgggtgcg agttagacgg ttaaataacg
     6661 tggattttcc tacgttaggg cgcccgacaa gcgcgaccac aggtaccatg tttaaagcct
     6721 catttttata aatcatcaga caacgcacgc tatattcgcg tcgttgttaa aaacaggaaa
     6781 acggcccctg tccaggagcc gttttcaaag tgaacgacag agacgattaa cgtgtaatag
     6841 agtacacggt tccgtctttt gcctggatca gcagtttgcc gtcagcggca accggttcag
     6901 tctggaaacc ggaactatca actttttgct gggcaacgaa acgaccatct tcgacattaa
     6961 tccagtgcag ataaccttca ctgtcaccga ccaccaggtt gccatcatac agcaccggag
     7021 aagtcagcag gcgatgcaac agatcgcttt gtgtccacag cgtaacgccg ccatcaatgg
     7081 tcaacgccat cacccggtca ttttgatcga ccagatagat gcgattgccg tcgacgatga
     7141 aatcattcac cgaacccagt tcgcgtttcc acataatctg accactgcgc agatcaagcg
     7201 ccgtcaggtt accattatag gccagcgcga aaacaacgcc gttaacaacg acgggagtcg
     7261 tgtcaacatc gctcagacgg tcaatttcgg tagaaccggt cgcctgggaa atacgctgct
     7321 gccaaatcat ctggccctgt tccatcagca ctgcgctgac gcgaccatta tcgcccccca
     7381 cgacggccgc accaaaagcc gttgccggcg cagactcgcc acgcaaagag agcgaaggca
     7441 tatcgaggtt aactgtccat ttgacagcgc cgtcagcttc gttcagcgct tgtaactgac
     7501 cgttactggt gtggattaac accagaccgt cgctgaccac cgggcgcgaa agtgcttcac
     7561 ccgcgacttt agtttgccat gccacagtac catcgctggt attcagcgcg taaacctgcg
     7621 ccttttcgct gccaatgtag acatgcccac cagacacggt cacaccgcca gaaagtaatg
     7681 caggctcttt agagaaccag ccatctttct cggccaggct gacagaccag atttctttgc
     7741 catcatccgc attcagcgct tttaccaaac cagcgcggtc cgctgcatag acaacgttgt
     7801 ccgccagtgc cggatgaaga ttggaataga agttgccaat gccgctacca acggaagtac
     7861 tccacgccgt ggtcggcgta aactggtttt caacggttgg caatggggac atctttacca
     7921 catcttcttc gctgttaaac agcgaacagc cgcttaaaag ggtaacggaa agcagtcctg
     7981 gcagcagtaa tttacgcaat tgcatcgggt ccctctcaga tggacaaatt attaattttc
     8041 atctgcatca tttcgctcag tgccggagta acatcgcttt tcacgcctgc ttcccatgca
     8101 ctacgcgcac cttgcttatc acctttgctc agcaatgctt caccacgcag gtcggcaaca
     8161 atggcagccc acccttcacc tttgatggta tcaagggttt tcagcgcggc atcagcctgc
     8221 ttgagctgta cctgaacgcg agcaagacgc agatttatca cggctttgag attttcatcg
     8281 ctcgtgtctg ccagcccctg ttgtaactgg gcggcagctt tctctagttc atttttgtca
     8341 acaaattgct gcgcaagttc caaagaagcc agcgcaccat aagtattttt attttcagca
     8401 gcaaattttt ccgccgccgg gatgctatcc ggtttgcctt cgctcactgc ggtaaccgca
     8461 ttttgatagg caagagaagc ggagcgtgca gaatcaacct gatggctgtt ccagtagcgc
     8521 cagccaatca gtgcgccaac gcccaaaatc accccaacag ccagtgcttt gccattttca
     8581 gcaaaaaagc gtttaaccgc ttctacctgg tcgttttcgt tctcgtaaat ttccacgctg
     8641 tccttctcct tccttaaccc agtaacgtgc gcaaatgcgc ggctacgcta tcctgcgcaa
     8701 ctgccgtttg ctcaccagag cgcaaatcct tcactactgc tgtgccgtta gtcacttcag
     8761 actcacccag caccacagca acgcgggcac cccatttatc agcacgggca aactgtttct
     8821 taaagttgcc gccgccgtgg ttggtcatca atttcacgcc ctgtaattca tcacgcagac
     8881 gctcagctaa tgccatagcc gcagattgtg tatcagcacc tgaagccacc aggtatatat
     8941 cgacaatagg atcggcttta aattccggat taacggcctg tactaacaat acaagacgtt
     9001 cgaggcccat cgcaaaaccg acagccggtg ttgcacgacc gcccagttgt tccacaagac
     9061 cgtcataacg accgcctgca cacacggtgc cctgggagcc gagactgtta gtcacccact
     9121 cgaaaacggt acggttatag taatccagac cacgcaccag acgctggttt acggtgtaag
     9181 cgatccccgc gctctcaagc agtttgcaca gaccggcaaa atgctcacga gattcctcgt
     9241 ccagataatc acctaatgcc ggagcgtcgt tgagaagcgc ctgcacttcc ggattttttg
     9301 aatccagcac gcgcagcggg ttagtgtaca tgcggcgttt gcagtcttcg tccagctttt
     9361 ctttatgctg ctcaaggaat gccaccagcg catcgcggta attggcgcgt gcttccagcg
     9421 aaccgataga attcagctca agagttacgt gttcggaaat acccagcgcg cgccaccagc
     9481 gggcggtgag cataatcagt tcagcgtcga tatccggacc ttgcagaccg aaaacttcgc
     9541 agcccaactg atggaactga cgataacgcc ctttctgcgg acgctcgtga cggaacatcg
     9601 gcccgatata ccacagacgc tgttcctgat tgtacagaag accatgctcg atgccggcgc
     9661 gtacacagcc cgccgtccct tcagggcgca gagtcaggct gtcgccattg agatcctcaa
     9721 aggtgtacat ctctttttca accacgtcgg tgacttcacc aatcgcacgt ttgaatagcg
     9781 gggtctgctc tacaatcggc aagcggattt cactgtaacc gtagctgccg agcacgtttt
     9841 tcagtgtgcc ttcaatgcgc tgccagatgg ccgtttcgcc aggcaggtaa tcgttcatgc
     9901 cgcgaatggc ttgaatgttt tttgccacgt ttattctctt tctgaatata aaaatgaacc
     9961 ctcaacgctt ccctcaatgt ttggggagcc atgcgggttc aatcatacac gggaagcgag
    10021 gcgcttccca tcacgttatt atttttcaac ctgctgaacg tcaattcgac gcgcttcatc
    10081 cagctgactg gctttcgcac gaatgcgtgc ttccagctgg tcgatcatat cgttgttgtc
    10141 cagacggtct ttgcgcacgc catcttcata gaggccgctt ttcttgttgc cgccggtgac
    10201 gccgagtgta gaaaccagcg cctcacctgg gccattcacc acgcagccga taatagaaac
    10261 gtccatcgga gtgatgatat cttccaggcg ttgctccagc gcgttaaccg taccgataac
    10321 atcaaattcc tgacgcgaac aggtcgggca ggcgatgaag ttgatccctc gcgaacggat
    10381 acgcagcgat ttcaaaatat cgaaaccgac tttgatctct tcgaccggat cggccgccag
    10441 cgatacgcgc agcgtgtcgc cgatgccttc agacagcagc agacctaaac caatggcgga
    10501 ttttactgcc ccgctgcgtg caccaccggc ttcggtgatc cccagatgca gcggctgatc
    10561 gatctgtttt gccagcaaac gataagactc aacagcgagg aagacgtcag acgctttcac
    10621 gctgactttg aactgatcga agttcaggcg atcgagatga tcaacatgac gcatggcgga
    10681 ttccagcaac gcctgcggcg tcggttcgcc atacttttct tgcagatctt tttccagcga
    10741 tccggcgtta acgccaatac ggatcggaat gtttttatcg cgcgcacagt caaccaccat
    10801 gcgaatacgc tcttcattac cgatattgcc agggttaata cgcagacaat cgacgccgta
    10861 ttccgctact ttcagcgcaa tgcgatagtc gaagtggatg tcagccacca gcggcacgtt
    10921 aacccgctgt ttgatgagtt tgaacgcttc tgccgcgtcc atcgtcggta cggagacacg
    10981 gacgatatca gcgccaacgc gttccagcgc cttgatttga ttgaccgttg cttcgacatc
    11041 tgtcgtacgc gtattggtca tggactgtac ggcgatggga gcaccatcgc caatcggcac
    11101 attcccaacg taaatacgtg ttgattttct acgttgaatt ggagcctggt tatgcatgaa
    11161 aaatctcccg cgttacccgt ctgttactgc gccggtgatt gttcggcatt gagggtcaga
    11221 cgcgcaacct ggttagttct gataaaacga ctcagatcga caggtttccc ttgatactgg
    11281 atctgtactg cggctggcgc accaattttc agtttgtacg gtgcctggcc ggttaagttc
    11341 aaattaccgt ctttacgctg cataccgcta aacaattttt taccggtagc atcagtgacc
    11401 tccagccagc aatcggcagt aaagttcatc accagcgcat tcggatcagc caccggcgtg
    11461 gtcacgccag cctgatcggt tggcaacggc gcagcaccat ctggcgttgt tgctgccgtt
    11521 ggtgccgggg tcgcggcggt atcaacattt gcctgcgaag gcgaaacaac cgcattctgt
    11581 tgcggatcaa cagctggtgc tggcgcagtt acggcaggtg tttgcgtgtt ggttgcggta
    11641 gtatccacag acgctggcgg cgtggacgtt gtagccgggt ctgtagttgt cgacgtattt
    11701 aacggaacac tctgcccctg ctcgctatta ctgctcagtt ccgccgaaga ttgatcggcc
    11761 atagtggtga tctcttcctg ctgagctttg tggtcttgcc accaccaggc accgctcagg
    11821 ccgataacca caaacaacac cagccaggtg aaggtcatca gccagccgtc acgttttttg
    11881 cggcgtttac cgagggaaaa actctgcatc ggcgcaactt tcgcagcccg tagtggagcc
    11941 tgcttttcca gccctagcag cagttcttct tctggaatat gtaccagacg cgcataagag
    12001 cggatatatc cgcgcaggaa tgttgaagca agatcggcgg gtgccttatc ttcttcaatg
    12061 tcgcgtaccg tggaaacctt caggcaaagt cgctcggcaa cggcctgctg actaagtcct
    12121 agttgttcgc gagcattacg caggcgagcg ccggtagtaa gtgcttcatt ttggtcgtgc
    12181 gtggcttcag tattcattcg ctgcaggtac gtttaaatga gaattgagat gccggtgaac
    12241 catcatgctc acccacaccg cgaaacatcc gttaagttaa ccgttatcat acagtataag
    12301 accgtctgtg tgctcgtgac aaagcccaca ttcactaagg ctaacttact gttgcatcgt
    12361 tacatactgc cttaaagtca gcaaaaacgc accgttaata ttgaccagac aattgcaact
    12421 taatacttca ttcataaaag attacgccac ggtacataaa gtaaccgtgg cgtaatggct
    12481 atcagaccgc tttaatgtcg atggcttcac cctgcatccg tttacgcagg gtacgtttcg
    12541 tacggtcgat aacatcgccc gccaactgac cacaggcagc atcgatatca tcaccacgag
    12601 ttttacgcac aatagtggtg aaaccgtagc tcatcagcac ttttgagaaa cggtcgatac
    12661 ggctgttcga actgcgtcca tacggcgcgc ccgggaacgg gttccacggg atcaggttga
    12721 tcttacacgg cgtatctttc agcagctccg ccagttggtg cgcgtgttca gtgccgtcgt
    12781 taacgtggtc aagcatcacg tattcaatag tgactcggcc ctgattggcg ttggatttct
    12841 ccagataacg gcgcaccgca gcaaggaacg tttcgatatt gtactttttg ttgatcggca
    12901 caatttcgtc acgaatttcg tcgttcggcg cgtgcaggga aattgccagt gcaacgtcga
    12961 tcatatcgcc cagtttatcc agcgccggaa ctacaccgga agtagaaagc gtgacgcgac
    13021 gtttagacag gccaaaaccg aaatcatcca gcatgatttc catcgccgga acgacgttgt
    13081 tcaggttgag cagcggctcg cccatgccca tcatcactac gttagtgatt gggcgctgac
    13141 cggtgacttt tgctgcgccg acgattttcg ccgcacgcca cacctggccg ataatttccg
    13201 acacccgcag gttgcgatta aagccctgct gggcggtgga acagaattta cactccagcg
    13261 cacaccccac ctgcgaagag acgcagagcg tggcacggtc gtcttccggg atatacaccg
    13321 tttcgacgcg ctgatcgcca acggcgatcg cccatttaat ggtgccgtcg gatgaacgct
    13381 gttcttcaac cacttccggt gcgcggattt ccgccacctc tttcagtttg ccgcgcaaca
    13441 ctttgttgat gtcggtcatc tcatcaaagt tgtcgcagca atagtgatac atccacttca
    13501 tcacctgatc ggcgcggaag ggtttttcac ctaaatcttt aaaaaactcc cgcatctgct
    13561 gacggttgag atccagcagg ttgatttttc catctttcgt ggtgacgttt tcaggtgtga
    13621 ctaattgttc agacatatgc tattccggcc tcgttattac acgttatggc ccctggaggg
    13681 ttgaaaaaag aaacgccccg gtgagcggct gctcgtccgg gggcgctgca ttgtacaaat
    13741 tctggcgcac ggatgccacg tttgcacgcg gcatttacga aattattaac gggtgcgcgg
    13801 gcacacttcg ccttcgccaa agaaataagc gatttcgcga gcggcagatt cgacggaatc
    13861 agaaccgtgg gtaccgtttt cggtcaggct gtcagcgtaa tcagcgcgca gagtaccagc
    13921 cagtgcgttt gccggattgg tcgcgcccag cagatcgcgg tgacgctgaa cggcgttttc
    13981 accttccagc acggaaacca cgatcgggcc agaggtcatg aattcaacca gaccatcaaa
    14041 gaacggtttt ccatcgtgtt cagcataaaa gccacgtgcc tgttcaacgg tcaggtgcag
    14101 cattttggtg ccgacaattt tgaaccctgc agcttcaaag cgcgcaaaga tattaccaat
    14161 gacgtttttt gctaccgcgt tcggtttgat gatggaaaaa gtacgttcaa tagccatttt
    14221 tacctctgta aattgttctg ttgttgtctg taccagcgta cgagatggcg cggattataa
    14281 tgagcaacag ggccgttgac tattgatgaa ggtaacattt ttttaaaata aaatgaggtt
    14341 tagcaacaat cagggacaaa acagactatt gcatgacaaa tttcactgtc gcgatttgcc
    14401 ccacgtcatc catcaccagc aattgataat cacctttatc cgtcaaatgc agggtcacgt
    14461 tgcgcccgcg ttcagttaac ggttcgccat tcagaaacca ccagcgttca cctgcccctc
    14521 cacttgattg caacggcaaa gtcgcttccg ctgcgcccgg taaacgttta ataatcgcgc
    14581 catcgcgaac gcctgtcagc tgcagcggga gctgggcata gtgaccgtat ggcgggcaac
    14641 ttgtcgaggc cggtggtaag cgcacagcgc gacgttctga tgcgggcagc cagggttcca
    14701 gcggtagcgg ccagacattt atcatttctt gtcgcgcttg cgggcaatca gcggcaacac
    14761 gtttgccatt ttcatccagc cagatgggga aacgaatgcc attaatgcct tcctgctccg
    14821 gcagtaatag agttggcggc tgactcccgt ccagcagcca ggttgccagg cggcggcgac
    14881 agttaccgtc accttccggc aaagactgtc cgcccggcca gcagataacg ccacgagtga
    14941 ctgagttcgg gcgcgggtct tccggcagat tcgcactgcg cgacagtaag atattattga
    15001 cctgattcaa caatggtacg gcactggcaa agccaaactg accaacaacg ggcgtgccgt
    15061 ccggtctgcc agtccagatc ccaatgacat agcgagcgtt aaccccaatc gcccaggcgt
    15121 cacgatagcc atagctggtg cccgttttcc atgccagtgg ggcgacgcgc ggcaaggcac
    15181 tatccggcaa gggttgcgct tcatcagcca taatccggcg aatgatccac gccgcccccg
    15241 acgacattaa aggccattca agcagcggat cgtcaggctg taagcgcaat ttgcctgcct
    15301 tgccgtggcg agcaaacgcg gtatacgctg ccgccatatc ttccagtttt gcaccagcgc
    15361 cgccgagaat gagtgaaaga ttcggcgcag caccgttggg caaatataac ggcaatccaa
    15421 cattgcgtaa ctttgccgca aaccgtttcg gtccataggc ttccagcacc tgcacagcag
    15481 gtaagttcag cgagcgcacc agcgcctcgc tcatgctgat cgggccatga aaaccgctat
    15541 caaagttacc tggtcgataa tcaccggtgc gccgggggac gtcttgcagc agtgatgccg
    15601 ggtggatcaa gccttcatcc agcgccagac cataaacaaa cggtttgagc accgatcctg
    15661 gcgatcggat cgcattgacc atatcaacat gaccaaagcg tgaatcatcg ttgagatcaa
    15721 ccgatcccac ccagccgcga acacgcatat cggtatgatc aaccacgatc atcgccagtg
    15781 agctgcgcgg tggcaaccgc cctttccagt tttgcgccag ttcttccaga cgtcgttgaa
    15841 gaccggcatc cagcgtagta acgattttgt cgcttttgct tttaccgagc atcatgcgcg
    15901 aaaacagcgg tgccagttgc ggcatttgtc ggggtgccag ccagatgggt tcttcccttg
    15961 actcttttac ctgctcacgg gaccacacac cttgcacggc catccgttcg agcactttat
    16021 tacgcgcggc ttcggcacgc tccggccaac gatccgggcg cagtcggctg ggcgcttgcg
    16081 gtaacaccgc cagcattgcc gcctcggaat agcttaaatt cgcaggcgat tttccgagat
    16141 aagcccaact tgccgcaccg atcccctgca acgtaccgcc aaacggagcg cggttaagat
    16201 acaagatcag aatttcacgc ttagacaaat gccactccag ttgcaacgcg cgccagagct
    16261 ggagaatttt gccaccaaat gttttggggt gaggatcaag cagacgagca acctgcatag
    16321 tgagcgtgct gccaccagaa ataacccgtc ccgaagtgag atcttgccat gctgcgcgcg
    16381 ccaccgagaa tggattcacc cccggatgct tccagaacca gcgatcttca taattgatca
    16441 gcgcttcaag gtaacgtgga gacacatctt cgattgttac cggataacgc cagatgccat
    16501 cagcatcggc gaagcgccag agcggcgtac catcctgcgc cacgaccact cgtgcgggat
    16561 tgacttcatg cagcggtaga ggccagagtt tatctgctcc ccacgctgca agaaaaagga
    16621 gaaatggcac agctgccagc gttatccagc agccgcgttt ggttaacaag cgaagcattt
    16681 acggtcggac aatcagcaga tcttcagccg cgccggtcgc ccgccattgg ggaacataca
    16741 ttgattccac catcggttgc ggtacctgat acgttcccgg cgtcaccgcc cgcgccagat
    16801 acaccagcgt taccggttgg tattcatcaa cggcaaccgc cgccacaaag cgatcgtcac
    16861 ggaactcaat gtgcttaatg ctcgcctgct gcatctggtt cagtaagttt tgcacttcgt
    16921 caccactttg ctccaggctg gcgctaccgt tcgccagatt ctgattttcc agttccagac
    16981 ccgcaggcag cagatccacg actaacgcat ccggcacgct gttactggct tttacctgca
    17041 accagaccag caccagatcg ccgctacgta acgagtccag cgatttgctc ttaccatcag
    17101 taccaagaat atgacgctcg atttgcagca cattgttcgc cggtaaaggt gcggattgcg
    17161 gataaccgct ggcatccata cgcaaccata acggctgatc gccactgttt ctcacctgca
    17221 aggtggcaag ttgatcgcta ttcagattgc tgtttagcgt tttctcacct gtcagcggct
    17281 cagctgagaa agaggtttgc gcctgccatt tacccggtaa atcctgaatc gtacgggcag
    17341 ccaggaacaa cgcgttactt tcctgcgtcg atagccagcg ttcaccaaac gcctgctgcg
    17401 aaagtgtgtt cagcaaagtg tactgctcat cgggtagcag tttattttct tccagcaagg
    17461 agagcattaa cgcgttgtcg cgcagtggac taccgtaatc acccagccat atccgctcat
    17521 cactatttct cggcgttttc agcgccagca caatcgcttc ttcaccacgc gttgcatcac
    17581 ccatggtttt cagcgcaatg ccaagttgca gcagcggtaa accagaagcg gcatctgcac
    17641 gatgctccca gatttcacgc agcgcaccca gtggtgcttt ttgctgacgg gccagcacca
    17701 gcgcagcgta agactgaacg gcgaatttac tggctttgag attatccgcg tacgggatcg
    17761 acatcatgcc cggatcttgt aaatagcgca gcagacgctc attaccccgg ttaatggcgt
    17821 ctgtcggcac gctgtaaccc tgttcgcctg cgcggaccag gaaatccatc acgtaagccg
    17881 tcagccagta ctcttcgtca ccgtttttat cccacagcgc aaagccgccg ttatcacgtt
    17941 gcatttgcag caaacgggaa atgccgatat cgaccgatgc acggcgtttc tcatcactgt
    18001 cgcctttgat gcccaacgcc tgcagttggg ctgcgttggt ataaagtgac gggaacaggc
    18061 cgctggcggt ttgctcaaga cagccgtacg gatacgcttt taactctttg atataacgtg
    18121 caatgttcag cggtggtttg ccgctcaaca acaattgccc ttccagcgta acaggcgaga
    18181 agttttgcaa tccatccgcc ggaatcgccc atgtctcacc aggctgtaac gccgtaccgt
    18241 aattaaccgt ttgtgccggg aacgccggac ggacgccgat tttccactgc ttatgctgat
    18301 cggcaacggt ttcacccggt aacgctaacc cgctaatggt ggcctgaatt tctccatcgc
    18361 cataacccgg caatgctcgc accgggataa acagcgtagt acgcacacct ggcgctaatt
    18421 caacggctgc gggtgaatcg ctgaccagtt caagcaaacc actggcggtc agggcaacgt
    18481 tcagtttttg cggtttatcg gtaagattag tgatatccag cgtcagacgc gaggtatcgc
    18541 cactcgccat aaagcgcggc atgttcagtt cagcaatcac cggtgcggca actatcactt
    18601 tgctttcgtt gctaccgaaa tcatctgccg tccaggcttg cgccatgacg cgcaattcac
    18661 cgttaaaatc gccaatcggc agtgtaaccg agccttcgcc ctgttcgttg agcgttaccg
    18721 gcagcgcctg ctgcgcgaca atattgacgt gattgaccag cggtttacca ccacgtttca
    18781 gctcatcacc atcgccaccg aaacgcagag ctgccagacg cccctgacct tcaataacct
    18841 gaccgtaaat atcgtaaatg tctgcgccat agcgtttctg accaaagaac gcctgccacg
    18901 gatctggcgt gacgtagtca gtaatattca gcacaccact atcaacggca gacaccagca
    18961 cattcacctg tttaggcttc tcgccatttt tagtgctggc tttaattttc acggttaatg
    19021 gttgattggg acgcattttt gctggtgttt ccagcgccag atcgaggcga cggttttcat
    19081 cgccaagcgg cagatgcaac acaccaaccg cgcgttttgg cgtcgcggag cgagatttat
    19141 cgctaggacg taccaccagc gtacttaaat acagatcatg acgattccag gttttatcga
    19201 ccggaatcgt cagatccagc ccttgagccg gaacatcaat ctcttgccac cacagcggcc
    19261 cttcactgga ctcgaccatc gcataacctt tacccgccgt tggcgcggcg atatgcaact
    19321 taatggtgtc gccagggcga taactggctt tatccagttt cagcgtgaca cggtcgggtc
    19381 gcactgcgcc gctaccgtcg ctgttgtcct gccaactata gccagcccag aaacgaacac
    19441 tactgaccgc ttcattcggc gctttgactt ccagacgata agcgccccac tctaccggaa
    19501 aactgacctt gccggtttcg tccgctttca gatccagagt ttgttcattt tcgatcagat
    19561 ctttttgatc aaactgtgac tgccagcctt catcttctga ccagttccag tagtaatcgc
    19621 ggcgttcgcg aatcaggcgc acctgcaagc ccgacacggc ttttttcacg ccttgtgcat
    19681 cgctataaac gatgtcaaaa gcggcgttac tgccttcatc aacaatgggc tgtttcaccg
    19741 tgctgtcagt gcgataatcg taaaccgatt tcaaggcgaa ctgcggacgg agccccggca
    19801 atgcattggc aggccagata gcctgctcag cgcggcgcgt caccgggcga ccgcccgatt
    19861 ccagcaaact gccctggaaa ataacctgca atggggaatg cgtttccttc cactggcttt
    19921 ctgtagaaac ttcgccgcgc cctttatcat ccagcgtcaa ctgaacttca tccagcgtgc
    19981 gggaaaggtt ttcggcagct atatcgccga attcaaaacc aggtaaggct gacacagcct
    20041 cacgcagtgg gcgcaggaaa agttgccctt gcaaagtatt accattagca ggcgcaccgt
    20101 acaggtagta tcccaccacg gagaatttca cttcatcatt cggcgtaagc ggggttttct
    20161 caccggtcag attcagcgcc atacgctctg gcataaaatc ttcgacgtgg aaatcccaca
    20221 tccggtactg gttatcgccc gtgttggcgc gaatatgcca cataccggtt gccgcattgc
    20281 tatcgagtgg ccaggtaaag tggtagaggc cattctccgg ctgactaacg acgctcctga
    20341 gtacctgccc gtcaggttta atcacgtcta acttgatggg ttgatcgggc aatgctttac
    20401 cgtctgcatc gcgcagtaaa ccattgagga ttaccgtttc acctgggcga taaagatcgc
    20461 gtgggccaaa catgaaaaac tgtttgctat agcctggcgc gccagcaatg ttaaattctg
    20521 ctaagtccag cgccggaagt tttaaatcga gtagcgttgt ctgaccgtct ttacgtgcca
    20581 gcagtaatgc cgcattttta tcattttcca gctgcacatg cccctgagcg tcactggttg
    20641 cctgagtcag agtctgccct ttctcattta ataaagagac ttcaattcct tgctgggccg
    20701 cgccgttttc cagactttgg gtaaagatat ccagacgatt gtgataacgg tgagctgaaa
    20761 cgccgatatc acttaacgta aacagcgtcg cgggattact gtaatcgtaa cgtccagcct
    20821 gattcatcac agccagatac acgcccgcct gctgaagcgg tttgatatcg cccagcggca
    20881 gcaataattt ttcacgggtg ttacgcgcag gattgagatc aaaccgtccg gtgtagacca
    20941 gatccgccat ctgcagcagt ttgtctgact gccagttcgc cagcgaattg cggtattccc
    21001 attggctaat gaatgctggc agagattctg gcttaacgcg gaagaagtta acatcgacat
    21061 tattaacgtt gagcgccatt accggcagcc cttcaacgac tttgccaggc agcagcgaac
    21121 cacggctggc aaaaccgacg ctgggttgga tgtcgcgggt agttatagtt ttttcgtaat
    21181 ctttactgaa ggttgcgttg ttgagtgctt tgacctcctt gccaatagta acgatcaaat
    21241 cacgtttcgg ttcgaggtgg cgtaaacgta gctctttaag attatctgac agctcccagg
    21301 caccatccac tttgccgctt tttttatcga cgacatgaat aacgcgtgag aaatcctgat
    21361 c
//
LOCUS       DAJTOR010000060        20754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTOR010000060 DAJTOR010000000
VERSION     DAJTOR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.060"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(11..556)
                     /gene="hemG"
                     /locus_tag="OO249_001967"
     CDS             complement(11..556)
                     /gene="hemG"
                     /locus_tag="OO249_001967"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5636281.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OO249_001968"
     CDS             complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OO249_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5636282.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2058..2672)
                     /locus_tag="OO249_001969"
     CDS             complement(2058..2672)
                     /locus_tag="OO249_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5636283.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGIGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OO249_001970"
     CDS             complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OO249_001970"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5636284.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4193..6382
                     /gene="fadB"
                     /locus_tag="OO249_001971"
     CDS             4193..6382
                     /gene="fadB"
                     /locus_tag="OO249_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5636285.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6392..7555
                     /gene="fadA"
                     /locus_tag="OO249_001972"
     CDS             6392..7555
                     /gene="fadA"
                     /locus_tag="OO249_001972"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5636286.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCN
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7747..8448)
                     /gene="fre"
                     /locus_tag="OO249_001973"
     CDS             complement(7747..8448)
                     /gene="fre"
                     /locus_tag="OO249_001973"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5636287.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8494..9987)
                     /gene="ubiD"
                     /locus_tag="OO249_001974"
     CDS             complement(8494..9987)
                     /gene="ubiD"
                     /locus_tag="OO249_001974"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5636288.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10154..10642
                     /gene="rfaH"
                     /locus_tag="OO249_001975"
     CDS             10154..10642
                     /gene="rfaH"
                     /locus_tag="OO249_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5636289.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10639..11421)
                     /gene="tatD"
                     /locus_tag="OO249_001976"
     CDS             complement(10639..11421)
                     /gene="tatD"
                     /locus_tag="OO249_001976"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5636290.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELASQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11463..12239)
                     /gene="tatC"
                     /locus_tag="OO249_001977"
     CDS             complement(11463..12239)
                     /gene="tatC"
                     /locus_tag="OO249_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5636291.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12242..12757)
                     /gene="tatB"
                     /locus_tag="OO249_001978"
     CDS             complement(12242..12757)
                     /gene="tatB"
                     /locus_tag="OO249_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5636292.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12761..13030)
                     /gene="tatA"
                     /locus_tag="OO249_001979"
     CDS             complement(12761..13030)
                     /gene="tatA"
                     /locus_tag="OO249_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5636293.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13109..14749)
                     /gene="ubiB"
                     /locus_tag="OO249_001980"
     CDS             complement(13109..14749)
                     /gene="ubiB"
                     /locus_tag="OO249_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5636294.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14746..15351)
                     /gene="ubiJ"
                     /locus_tag="OO249_001981"
     CDS             complement(14746..15351)
                     /gene="ubiJ"
                     /locus_tag="OO249_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5636295.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15365..16120)
                     /gene="ubiE"
                     /locus_tag="OO249_001982"
     CDS             complement(15365..16120)
                     /gene="ubiE"
                     /locus_tag="OO249_001982"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5636296.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16215..17642)
                     /gene="rmuC"
                     /locus_tag="OO249_001983"
     CDS             complement(16215..17642)
                     /gene="rmuC"
                     /locus_tag="OO249_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5636297.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17783..18544)
                     /gene="udp"
                     /locus_tag="OO249_001984"
     CDS             complement(17783..18544)
                     /gene="udp"
                     /locus_tag="OO249_001984"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5636298.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18806..19618
                     /locus_tag="OO249_001985"
     CDS             18806..19618
                     /locus_tag="OO249_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5636299.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGMHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            20012..20672
                     /gene="hcp"
                     /locus_tag="OO249_001986"
                     /pseudo
     CDS             20012..20672
                     /gene="hcp"
                     /locus_tag="OO249_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680647.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5081 a         5603 c         5210 g         4860 t
ORIGIN      
        1 ttctttatgc ttatttcagc gtcggtttgt tggttaactg ggcgatttct cgggcgaaat
       61 tcgccacctg ctcccaatcg gtatagacaa cttctttgcg cgtatccgtt tcaccgcctg
      121 acatcttcat aatcagctta atcataaaac ggtcgtacca gcgataacgt gggtaacgca
      181 gcgccccggc aatgaccgcg cagcgatcgg gacgccattg tgaactcatc agaaacttgc
      241 gcgcgtagct gttggtctgt ggagtacgct tctccggttt gcgcgccacc aggtttacgg
      301 aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg ttttttgaca aattcctgga
      361 acgcagagtg gtagtgacca tagcgaatag aagcaccaat gaccacacgg tcatagtttt
      421 cccattgtgg ttcttcaatg cggtgcacat tggcgacatc cgtctggatc cccagttctt
      481 tcagttccga agccaggtag gaggcaatct cgcgcgtttg tccgtccctt gttgagaaaa
      541 gaattaatgt tttcacgtat tactccatca ttcacgccag aaagtcgggg taaagagcac
      601 cagcaatgta aagacctcga gacgaccaaa cagcatgttg gcaatcagga tccatttagc
      661 caccgggttc atactggtaa agttatcagc aaccacgcca agccctggcc ccaggttatt
      721 caatgtcgca acaactgagg caaaggcaga aaaatcatcc acgcccgtgg cgataatcgc
      781 cagcatactg acaataaaca ccaatgcata ggcggagaaa aatccccaaa cggcttcgag
      841 gatacgttcc ggcagtgcgc gattccccag cttaatgcta tagacggcgt tcggatgcac
      901 cagtcgtttc agctcacggt tcccctgctt aaacagcagc aggatgcgga tcactttcag
      961 gccaccgccc gttgacccgg cgcaaccgcc gataaaagct gaacataaaa gcagtaccgg
     1021 caaaaagagc ggccagcggg caatgctgtc agtcgtaaac ccggcggttg tcgccatcga
     1081 taccacctgg aaaaacgcct ggttaattgt catcagcgcc gaactgtaga cattatgaaa
     1141 ccacagtacg agtgtacaaa taaccaccag cgtaaactgt acgccgataa acatgcgaaa
     1201 ttccggatcg cgccaataaa ccttcagact acgcccactt aacagtgaaa agtgcagacc
     1261 gtagttacaa ccggagatca gcaggaagat agcaatgata gtgttaatag tcggactgtc
     1321 gaaataaccg atactggcat catgtgtcga gaagccgcca atagcgatag tcgcaaagct
     1381 atggccgatg gcatcaaagg catccattcc ggcaaaccac aacgccagcg cacaggcgac
     1441 ggtcagcaag acataaatca accacagggt tttcgccgtt tccgcaattc gcgggcgcat
     1501 tttgttatct ttcagcgggc cgggcatttc tgcgcgatag agctgcatcc cacccacgcc
     1561 gaggataggc agtatcgcaa ccgctaacac gatgatcccc atcccgccaa accattgcag
     1621 catctggcga taaaagagga tggcatgagg gagcgaatcc agccccacca gcgtagtggc
     1681 cccggtggtg gtcaggccag agaaagattc aaaaaacgca tcggtaatcg tgaggttcgg
     1741 gctttccgag aagataaaag ggagcgcacc gacactaccc agcacggtcc aaaacagcac
     1801 cactatcaga aacccctcac gggatttcag ttcgcccttc tctttgcggt tcggccacca
     1861 cagcatagag ccaatggcga gggcgacaaa aaaggtctgg gtaaaagcgc ggcccgctcc
     1921 atcccggtag atgagtgcta ccagcccagg gataatcatg gtccctgaaa ataagatgac
     1981 cagtagtcca acgattcggg taatggcgcg aaaatgcatc tctgccgctt ccttagttct
     2041 gcaaaatgaa gtggggatta ttcttcaatc gctaacaatt gcaatgaacc acggctaaaa
     2101 tccgccagct ttgcggaaaa ttcagccact ttcgccgccg gaagcgccac ccgcagcaga
     2161 acgaatgcct gataatcact gttgataatt ttgccgtcac actgccccag caacgcttca
     2221 atgccggtta actgactata ttcacattgc aaagtatatt cggttaatgg cgtcttgcgt
     2281 tgggtcgtta gctggcgcag cgcctgattc acgccgccgc catacgcttt cactaaccca
     2341 ccggtgccaa gcaatatgcc gccgtagtag cgcactacca cagcggtaat ttccccgatg
     2401 ccgctgccca ttagctgggc gagcatcggt ttacctgccg ttcccgccgg ctccccgtcg
     2461 tcagagaaac ccagctgttg agaatcatcc ggcgcacccg ccacccacgc cacgcaatgg
     2521 tggcgggcat cggggtgttc tgcccgcacc gattcaacaa acgctttcgc cgcctcaacg
     2581 ccatcggtat gcgccaacat cgttatgaag cggctctttt tgatctcttc aacgaccgtg
     2641 accggtgccg caggaattaa ccagctttcc atcacgccag tttcagatcc cgggtcatgt
     2701 tttccacgtt attttcgtgg atcaccacgt tgtcttcgat acgaatgccg ctgaacggtt
     2761 tcagtgcttc aattttctgc cagttgaagt gcttgctgaa ctgcccttca cgccacggtg
     2821 ccagtagcga ttcgatgaag tagatacccg gttcgatggt taacaccatg cccggctgga
     2881 gaatacgggt gcagcgcagg tacggatatt ttgccggtgc cgcgaggtgt gtaccgctat
     2941 catcctgcat aaaaccggct acgtcatgca cctgcaggcc cagcggatgg ccgataccgt
     3001 gcggcataaa cggtccggtg agatcgtttt cgaccatcgc ttcttcactc atatcggtga
     3061 tgatttgatg tttacgcagc aatttggcga tgcgctgatg gaactggagg tggtaatcca
     3121 catagctgac gccagctttc atggtagcga tcagcgcaag ttgttcatca tttacgtctt
     3181 tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca ggtacgagtc aggtcagccg
     3241 catagccgtt atattcggcc ccggcatcca gcaggaagct gcgcatctct tccggtgcct
     3301 gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc gttaagcgcc acaatgttgc
     3361 tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag ataggcgatg ttgatatcga
     3421 actcgctcat gccagaacgg aacgcttctt ctgccgcacg atgaccgttg accgccattt
     3481 tctgcgcttc acgcatacag gccagttcgt actcggtttt gaaggagcgg tagtaatgca
     3541 ggtagtcgat cacccctttc gggttgatgt tgctggcctc aatacccagt tgcagcgcac
     3601 gttccggcac cggaccgata taaccgatat tgccgcgcgc agcgggcagc aggctaccaa
     3661 tgccatcggc tttcggcagt gcgatcactt ccacatcttc agtccagaag gaggtcggca
     3721 gcggttcgac gttgtgccag taatcaaccg gcagatagaa ccacagtttc ggcttgttca
     3781 cgccatccac cagcaaccag cagtttggca cctgagttac cggcacccac gctttgaatt
     3841 gcgggttcac tttaaacgga tagggatgat cgtcgagaaa aacgttgaac agctcgccgg
     3901 agtgaataag taatgcatcc agcttgaagc gcgccagcgc atcgcgagtc cgttcttgta
     3961 aggtagctat atgattttta tagagcgagg ccagtgattc cattttttac ccttctgttt
     4021 ttttgacctt aagtctccgc atcttagcac atcgttcatc cagagcgtga tttctgccga
     4081 gcgtgatcag atcggcattt ctttaatctt ttatttacat gtttttaaca caaaatacac
     4141 acttcgatgc atctggtacg accagatcac cttgcggatt caggagactg acatgcttta
     4201 caaaggcgac accctgtacc ttgactggct ggaagatggc attgccgaac tggtatttga
     4261 tgccccaggt tcagttaata aactcgacac tgcgaccgtc gccagcctcg gcgaggccat
     4321 cggcgtgctg gaacagcaat cagatctaaa agggctgctg ctgcgttcga acaaagcagc
     4381 ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg ttcctcgttc ctgaagaaca
     4441 gttaagtcag tggctgcact ttgccaatag cgtgtttaat cgcctggaag atctgccggt
     4501 gccgaccatt gctgccgtca acggctatgc gctgggtggt ggctgcgaat gcgtgctggc
     4561 gaccgattat cgtctggcga cgccggatct gcgcatcggt ctgccggaaa ccaaactggg
     4621 catcatgccg ggctttggcg gttctgtacg tatgccacgt atgctgggcg ctgacagtgc
     4681 gctggaaatc attgccgccg gtaaagatgt cggcgcggat caggcgctga aaatcggtct
     4741 ggtggatggc gtagtcaaag cagaaaaact ggttgaaggc gcaaaggcgg ttttacgcca
     4801 ggccattaac ggcgacctcg actggaaagc aaaacgtcag ccgaagctgg aaccactaaa
     4861 actgagcaag attgaagcca ccatgagctt cactatcgct aaagggatgg tcgcacaaac
     4921 ggcggggaaa cattatccgg cgcccatcac cgcagtaaaa accattgaag ctgcggcccg
     4981 ttttggtcgt gaagaagcat taaatctgga aaacaaaagc tttgtcccgc tggcgcacac
     5041 caacgaagcc cgcgcactgg tcggcatttt ccttaacgat caatatgtaa aaggcaaagc
     5101 gaagaaactc accaaagacg ttgaaacacc gaaacaggcc gctgtactgg gcgcaggcat
     5161 catgggtggc ggcattgctt accagtctgc gtggaaaggc gtaccggttg tcatgaaaga
     5221 tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc gcgaaactgc tgaacaagca
     5281 gcttgaacgc ggcaagatcg atggtctgaa actggctggc gtgatctcca cgatccaccc
     5341 aacgctcgac tacgccgggt ttgaccgcgt ggatgttgtg gtagaagcgg ttgtcgaaaa
     5401 cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa aaagtacgcc cggataccgt
     5461 actggcgtct aacacttcaa ccattcctat cagcgaactg gccaacgcgc tggaacgccc
     5521 ggaaaacttc tgcggaatgc acttttttaa cccggtacac cggatgccgt tggtagaaat
     5581 tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa gttgtcgcct gggcgagcaa
     5641 gatgggcaag acgccgattg tggttaacga ctgccccggc ttctttgtta accgcgtgct
     5701 gttcccgtat ttcgccggtt tcagccagct gctgcgcgac ggcgcggatt tccgcaagat
     5761 cgacaaagtg atggaaaaac agtttggctg gcctatgggt ccggcatatc tgctggatgt
     5821 cgtgggcatt gataccgcgc atcacgctca ggctgtcatg gcagcaggct tcccgcagcg
     5881 gatgcagaaa gattaccgcg atgccatcga cgcgctgttt gatgccaacc gctttggtca
     5941 gaagaacggc ctcggtttct ggcgttataa agaagacagc aaaggtaagc cgaagaaaga
     6001 agaagacgcc gccgttgaag acctgctggc agaagtgagc cagccgaagc gcgatttcag
     6061 cgaagaagag attatcgccc gcatgatgat cccgatggtc aacgaagtgg tccgctgtct
     6121 ggaagagggc attatcgcca ctccggcgga agcggatatg gcgctggtct acggcctggg
     6181 cttccctccg ttccacggcg gcgcgttccg ctggctggac accctcggta gcgcaaaata
     6241 cctcgatatg gcacagcaat atcagcacct cggcccgctg tatgaagtgc cggaaggtct
     6301 gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg gttgagccag cccgtccggt
     6361 tggcgacctg aaaacggctt aaggagtcac aatggaacag gttgtcattg tcgatgcaat
     6421 tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt aacgtgcgtg cagaagatct
     6481 ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg gcgctggaag cggcggccct
     6541 cgacgatatt tactggggtt gtgtgcagca gacgctggag cagggtttta atatcgcccg
     6601 taacgcggcg ctgctagcag aagtgccgca ctctgtcccg gcggttaccg tcaatcgctt
     6661 gtgtggttca tccatgcagg cactgcatga cgcagcacga atgatcatga ctggcgatgc
     6721 gcaggcatgt ctggttggcg gcgtggagca tatgggccat gtgccaatga gtcacggcgt
     6781 cgattttcac cccggcctga gccgcaatgt cgccaaagcg gcgggcatga tgggcttaac
     6841 ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa atgcaggatg cctttgccgc
     6901 gcggtcacac gcccgcgcct gggccgccac gcagtcgggc gcatttaaaa atgaaatcat
     6961 cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag tttaattacg acgaggtgat
     7021 tcgaccggaa accaccgtgg aagccctcgc cacgctgcgt ccggcgtttg atccagtaaa
     7081 cggtacggta acggcgggca catcttctgc actttccgat ggcgcagctg ccatgctggt
     7141 gatgagtgaa agccgcgccc atgaattagg tcttaagccg cgcgctcgtg tgcgttcgat
     7201 ggcggtcgtt ggttgtaacc catcgattat gggttacggc ccggttccgg cctcgaagct
     7261 ggcgctgaaa aaagcggggc tttctgccag cgatatcggc gtgtttgaga tgaacgaagc
     7321 ctttgccgcg cagatcctgc cgtgcattaa agatctcggg ctaatggagc agattgacga
     7381 gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat ccgctgggct gttccggtgc
     7441 gcgtatcagc accacgctgc tgaatctgat ggaacgcaaa gacgttcagt ttggtctggc
     7501 gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg tttgagcggg tttaaccact
     7561 tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc ctgcaccaat gtaggcctga
     7621 taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg acatagatct
     7681 aaatgccact gaataaagtt ttagttgccg ttcttcccgc ctgtcagggg cgggtttttt
     7741 tatatctcag ataaatgcaa acgcatcgcc aaacagacga tcttcccgcg cattacgctc
     7801 actgcaaaac aggtcacgag caattttcgc catctcaaaa cgtccggcaa tatagatatc
     7861 atgctctgcc agcgtaccat gatcctgcaa gaccgccgtt aacacggtgc cagtacgccc
     7921 acgccagccc gcttccggtt gttcaaccac cggcaccaca cgcaggccag gatgcttcag
     7981 cgaaagcgcc tcaagctcgg agagatcata aagatgttgc tcctcacgcc caccccagta
     8041 aatggtgata tcgcgattgg gattacgcgc caacgccgtc aataaaatcg agcgcgcata
     8101 ggagaacccg gtgccgcccg caatcaaaat catcggacgc tcttcatcat cgcgcagcca
     8161 cgcttctccg tagggaatat cgaccacgat ttgataatct ttgaggatgc ggtccatgac
     8221 tgctttcgcg taaaggttga tttcagaagc gccaatatgc agctctataa accctttttc
     8281 atccggcgtc gaagccattg aaaacggacg tttgtcgcgc tcatccatca ctaccatcaa
     8341 atactgacca gcacgaaaag aaaaggccgc gtctggcacg atgcggacac gatatacggt
     8401 atccgtgata gcttctaccg aggtcacttt acagcttaag gttgtcatgc gctttctctg
     8461 tcggatcgat aaatagggca aaacaaacgc gcatcaggcg cttttaccgt tgttaaaaat
     8521 agccagttca tcccagatgg cgtcaatatg cgcgacaaca tctggatctt ttttgatggg
     8581 acgtccccat tcacgctggg tttcccccgg ccatttattc gtggcatcca gccccatttt
     8641 tgaacccagc ccggagacag gcgaggcaaa atccagataa tcaataggcg tattttctac
     8701 cagaacagta tcccgcgccg ggtccatacg ggtggtaatc gcccaaatca catcgttcca
     8761 gtcgcgtgcg ttgacgtcat catcgcaaac gatcacaaat ttagtgtaca taaactggcg
     8821 taagaacgac cagacgccca tcatgacgcg cttcgcgtgt ccggcgtact gttttttgat
     8881 cgtcactacc gccagacgat aagaacagcc ttccggcggc aggtaaaaat cgacaatttc
     8941 cgggaactgt ttttgcagaa tcggtacaaa cacttcgttc aacgccacgc ccagtaccgc
     9001 gggctcatct ggcggacgcc cggtataggt ggaatggtaa atcgcatctt cacgctgggt
     9061 aatatgcgtc acggtaaata ccgggaaact atcgacttcg ttatagtaac cggtgtggtc
     9121 gccatacggc ccttccggcg cagtttcgcc ttgttcgata tacccttcca gcacaatctc
     9181 cgcactggcg ggcacttcaa ggtcattgga gatacacttc accacttcgg tcttggtacc
     9241 gcgcagcaat ccggcaaacg catactctga aagtgtatcc ggaacggggg tgactgcacc
     9301 gagaatcgtg gcgggatcgg cacccagcgc cacagaaacc gggaaacgtt cgcccggatg
     9361 cgccgcacac cactcctgat aatccagcgc gccgccgcga tgcgacagcc agcgcataat
     9421 cagtttgttt ttaccaatca gctgctggcg ataaatgccc agattctgcc gctctttatg
     9481 cgggccgcgc gtcactgtca gcccccaggt aatcagcggt gcggcatctt ccggccagca
     9541 ggtcataatg ggaatgcgat tgagatcgac gtcatcgcca gagacgattt tttgttggca
     9601 gggcacacca cgcagccgct ttgtcggcat gttcaatact tgcttaaact gcggcagttt
     9661 atcaaacagg tcgcggaaac cttttggcgg ctccggctct ttcagaaacg ccaataattt
     9721 accaacttca cgcagcgccg aaacatcttc ctgtcccatg cccatcgcca cgcgctttgg
     9781 cgtaccgaac aggttgcaca gcaccggcat tgagtagcct ttagggtttt cgaacaacag
     9841 cgcaggccca ccggcacgca aagtgcggtc agcaatttca gtgatttcca gatgcggatc
     9901 caccgggagc gtgatacgtt ttagctcacc ctgctgttca agcagcgtca agaagtcgcg
     9961 taaatcgtta tatttcatgg cgtccattgt agcctcttaa tctacgccca ttatacggcg
    10021 ttcatctttg cgatgctgta aatttgttaa attagcgtga actctgacgg tataacgcaa
    10081 accggggaat ataattaact tagcgtaaag cttttgctat ccttgcgccc cgattaaacg
    10141 gataagagtc attatgcaat cctggtattt actgtactgc aagcgcgggc aacttcaacg
    10201 tgcccaggaa cacctcgaaa gacaggctgt gaattgcctg gcaccgatga tcaccctgga
    10261 aaaaatcgtg cgtggaaaac gtactgcagt cagtgagcca ttgtttccca actacctgtt
    10321 tgtcgaattt gatccagaag tgattcatac cacgactatc aacgcgaccc gcggtgtcag
    10381 ccacttcgtg cgctttggcg cgtcgccagc gatagtccca tcggcggtga ttcatcagct
    10441 atcggtatat aaaccgaaag acattgtcga tccggcaaca ccttatccgg gtgataaggt
    10501 gattattacc gaaggcgcgt tcgaaggctt tcaggccatt ttcaccgaac ccgatggtga
    10561 ggctcgttcc atgctattgc ttaatcttat taataaagag attaagcaca gtgtgaagaa
    10621 caccgagttc cgcaaactct aaaacgcaat cccaaacagt gttttgacat tggcatccgt
    10681 ggtggcagcc agccatgcgg catcttctcc acgccagtgc gcaatacgtt gcaaaatatg
    10741 gtgcagatgg gctggctcgt tgcgccggga tgatggcttt ggcgtgaaat cgcgcgggag
    10801 cagatacggc gcatcagttt cgatcagcaa tttctccgcc ggaatcaacg gcaacaattc
    10861 ccgcagctcc agcccgcgtc gttcatcgca aacccaaccg gtaatgccga tataaattcc
    10921 acgcgccacg cacgcctgca tctcttcgcg tgtgccggta aagcaatgaa gaaccgcacc
    10981 aggcagttta tccagccacg gctccagcaa tgtcataaac cgctcgtggg catcgcgaca
    11041 gtgcataaat accggcatgt ttaattctgc ggcaatgcgt agctgagcaa caaaagcgcg
    11101 ttcctgctct tccggcgtcg aaaagttgcg gttaaagtcg agaccacatt caccaatcgc
    11161 caccacttct ggctgcgagg ccagctcaat aatcgcttct tcagtcgcag cttgccactg
    11221 gctgctgtcg tgaggatgta cgcccgccgt tgaccaacag gacgaatact gacgcgccag
    11281 cttttgcgcc tgctggcttt cacgcaggtt agtgccggtg atgagtagcc cattaactcc
    11341 cgcgtcaaaa gcgcgcgcta caacatcatc acggtctttc gcaaattgcg aactggtcaa
    11401 attaacgccg atatcaaaca tcctgtactc catatgacaa ccgccctgac gggcggttga
    11461 atttattctt cagttttttc gctttctgct tcagcgtcgt tttcctcttc ccgatttcgc
    11521 cctttaccaa cgtaaaagcg tgagaagaag acaccgattt caaacagaca gtacatcggg
    11581 atcgccaata gcgtttgcga gaagacatcc ggcggcgtca gcaacatccc gacaacgaat
    11641 gcaccaacca gcacatacgg gcgttttttg cgtaagtctt ctggcgaggt aatccccatc
    11701 cagcacagca gcacaattgc caccggcact tcaaaggaga caccaaacgc cataaacagc
    11761 gccataacga agcttaaata gctggcgatg tcggtggata cctgcacccc ttccggcgcg
    11821 gtattggcaa ggaagccaaa tgccagcgga aagaccacaa agtaggcgaa tgccatgccg
    11881 atataaaaca gcagagagct ggaaaccagc agcggcacca ccaggcgacg ttcatgctta
    11941 tacagcgctg gggcgataaa tgcccacacc tgatagagaa tcaccggcgc tgacagaatc
    12001 agcgacacca taaaggtcag cttgatcggc gtaaagaacg gcgaggccac gtcggtggcg
    12061 atcatcgttg aaccttgcgg caactgcttg atcaatggcg cggataccag gtgatagatg
    12121 tcattggcga aatagaccag acacaggaat atcacgatca ccgcgataat gcagttcagc
    12181 agacgcttac gcagctcaat tagatgcgtg ataagcggtt gagtatcttc tacagacatg
    12241 tttacggttt atcactcgac gaaggggaag gtgcagcggt tttcggttca gcgtccgcag
    12301 caggttttac caccggctct ggcgtggttt ctggcttctg ttccggcgaa ctggcctgcg
    12361 tttgtgcagc ggcaggcgtt acgccctcat gcgcagcttc attatctttc accaccgggt
    12421 tatggatggt gtgcgcttca tcgctcgcct tttctggatc gtttgcaacg taggaacgct
    12481 tcatcgactc cgcggcctgg cgtagttcat ccatcgacgc tttcagttcg ggcgtcaggt
    12541 tagtgaggct cgccttttca acctttttca gactgtcctg aaactcctgg agttttaact
    12601 cctggatcag ttcgttctgc accgttgtcg ccagtgaacg caacgcgcga atccagcccg
    12661 ctaccgtttt taccgccaca ggcagtcgtt gcggccccag aacgacgagg ccgatgatga
    12721 acaccaatag cagttcgcta aaaccgatat caaacacgga ttacacctgc tctttatcgt
    12781 ggcgcttcgc gtcttctgtt ttagcctgtt cctgattcgt atccgcctgc ttatcggcga
    12841 tagttttcgc agtaaaatca gcatcctgac tggttttatc ctgctttggt tcatcatcgc
    12901 tcattgcttt tttaaagcct ttgatcgacg caccaagatc ggaaccgatg gagccgagct
    12961 ttttggtgcc aaaaagcagt acaacgatga cggcaataat caataactgc caaatactga
    13021 taccacccat acatgttcct ctgtggtaga tgatgattaa acaaagccgc attatacgtt
    13081 acacggcccg ccttgagcga tgaaaaaatc agcgtgtttt gcgccaaccg acaaaccagg
    13141 cgatcagacc acctgccatt aaccagccgg gcatcagccc ccactcaggt cggctgacca
    13201 acaagaatgt gccacttaat actaacgtag cgccaattcc gagaaaataa cgcgattgtc
    13261 cctgacgtac atgatttgac tgaagctcgc gggcaatctt atcaacactg tgctgtaaat
    13321 acttgccctg gcgcaaactg tcgtaaacca attcaggcag ttctggcatt ttttcgaccc
    13381 agaacggcgc tttttcttta aatgctctca ccagcgcagg aataccgacc tgatctttaa
    13441 tccacgactc caggaaaggc ttcgctgttt tccataaatc gagctgcgga taaagctggc
    13501 gtcctacccc ttcgacatag agcagggttt tctggagtaa caccagttgc ggctgcactt
    13561 ccatattgaa gcgacgcgcc gtattaaaca gatttaacag tacatgtcca aacgaaattt
    13621 cggccagcgg tttctcaaag ataggttcac agaccgtacg aatggcaaat tcgaactctt
    13681 caacgttggt atctggtggc acccagcctg aatcgacgtg tagctctgcc actttgcgat
    13741 agtcgcgatt aaagaaggcg ataaagtttt ctgccagata gcgtttatct tctttgttta
    13801 gcgagccaac aatcccgcaa tcaatgccga tatatttcgg gttttccggg tgttcatagc
    13861 ttacgaagat gttgccaggg tgcatatcgg catggaaaaa gctgtcgcga aagacctgag
    13921 tgaagaacac ctgcacgccg cgttccgcca gcaatttcat gttagtgccg tttttctcca
    13981 gcgccgcaac atcagacacc ggaatgccgt aaatgcgctc catcaccatc atcccttcac
    14041 tacaatagtc agggtaaact tccgggatgt agagcatcgg gctgtcttca aaattgcgcc
    14101 gaagctgaat ggcgttggca gattcccgca gcaaattcag ttcatcaatc aaggtctttt
    14161 cgtactcgcg caccacttcg gttgggcgca gacggcgacc atccggcagc aaacgcggca
    14221 cccagcgagc cagacggtag ataagtttca gatccgcttt aatgaccggc aaaatatccg
    14281 ggcggatgac tttaatcacc acctctttac cattcgattt caatcgcgcg gtatgaacct
    14341 gggcgataga agcagaagcc agcggcttga tttcaaaatc gtcaaaccac gcttctaccg
    14401 gcaagccgcc cattgcagct tcaatctgct gcttcgccag cttgccatca aacggagcta
    14461 ctttgtcctg caataacgcc agctgatcgg caatatgcgg tggaaaaaga tcgcggcggg
    14521 ttgataacat ttgcccgaac ttgatccaaa ccggccccag ttcttgcagg gccagtcgta
    14581 gtcgctcacc taaaagtttg tctttatgcc gatttggcat ccagaataat gagtatcgcc
    14641 atagccgtag cggcagggtg atacgcattt tggggatcag ttcatcaagt ccgtagctta
    14701 aaaaagtgcg aatgatgaaa tataggcgcc gtacttcacc tggcgtcatt tagcctccag
    14761 tttttccagc cgtttggtca gggcatcaac agcacgctcg acggcagccg tctcttccgc
    14821 aaaccaggcc acttcaagcg gaccgggtgc catacgccac tcttcagtaa tggcttccgc
    14881 cacataacgt tgctggcgct taatgccgtg atgcaggaac tttgcgcctc cgcgcatggc
    14941 tttgctgatt ccttcagcgg cgatatcacc ggtataaggg gccagcagtt ccgcagggtc
    15001 gaactctgcc agatctgcca gcgcaacgaa gttttgcacc acctgaatat cgccctgcac
    15061 ttccagctca ccactgcgaa tcagtgcggt aagctgctgg cgatcgcgaa gtttcggcaa
    15121 cacactggcg taggcgataa cggtgcagtc agcatcgcct gcccattcgc ccagtacatc
    15181 aacctggcgt tcgctgaaca ccagaatcaa tgacgtcgaa aagcctttta cctccacgcg
    15241 caatacttta cccagcagac gcgagcgggc tgttttcagc gcgggtgagc gatacaggaa
    15301 ggtgttgagc agactttcaa ttcctgccgt cactaaaggt ttaaaaggca tttccggtct
    15361 cctgtcagaa cttataacca cgatgcagcg ccacaacccc tgccgtcaga ttgtagtagt
    15421 cgacactttc gaatccggca tcctgcatca tggctttcag ggtatcctga tcgggatgca
    15481 tacggatgga ttctgccaga taacggtagc tgtcggcgtc gttcgcgacc agtgagccaa
    15541 tacgcggcag cacatggaag gagtatgcat cataggcttt gctcagcggc tcgataattg
    15601 gcttcgagaa ctcaagcacc agcaggcggc cgccgggttt cagcacgcga tacattgaac
    15661 gcagtgcttt atctttgtcg gtgacgttac gcagaccaaa cgaaatggtg atgcaatcaa
    15721 aggtgttatc cgggaacggc agcgcctcag cgttcgcctg aacatactca acgttgccaa
    15781 tcacaccgat attacgcagc ttctcgcgtc ccattttgag catggattca ttgatatcag
    15841 caaggaccac tttgccagtt tctccgacca ggcgggagaa tttcgctgtc aggtcgccgg
    15901 tgccaccagc cagatccagc acggtctgcc cacggcgtac gccgctgcaa tcaatcgtga
    15961 atcgcttcca caaacgatga ataccaaatg acatcaaatc attcatgaca tcgtattttg
    16021 atgccacgga atggaaaacg tgggcgacca tatccgcttt ttgttccttc gcgacggtct
    16081 gaaaaccaaa gtgcgtcgtt tcttgtgact tatccaccat ctcaatgcct gctcatcaaa
    16141 aaattgttcc agaagtgtaa cagattgggc gtcgatgccc tagatttcta cccggcttaa
    16201 ctacccctaa tgggctagcg cgactgctga ttatattcat catcgcgttg ataagcttca
    16261 tcattcggct gctccggaac cgaccgaagt cgatactctt catcctggct caccgcctgt
    16321 tcagccaaat ccggattaat ctcgcgttta atttctactc ctaaaccgcg aaacgcttct
    16381 gcctgcgcca gcacatttcc gcgacctgaa gagagttttt tcattgcctg gcggtagtta
    16441 tcctgcgctt tgtcgagact ttgaccaatc gcggacatgt catcgatgaa caaacgcatc
    16501 ttgtcgtaca gcttgctggc acgatcggcg atttgctggg cgttgcggct ttgatgctca
    16561 taacgccaca ggttggcaat agtacgcaac gccaccagca gcgtagtcgg gctaaccagc
    16621 atgatattgt ttttcaacgc ttcggtgatc agctccggct ggcggtcaag cgccagtaaa
    16681 aaagcgggtt caacgggaat aaacatcagc acgtaatcca gagttcgcag ccccggcagc
    16741 tgttgataat ctttgcgtcc cagcaaacgg atatggttac gcaccgacgc gatatgttcc
    16801 tgtagcgcgc tttcgcgggt gtagtcgtct tcggcgttaa aatagcgttc ataggcgacc
    16861 agcgtcattt tggcgtcgat caccacatct tttccctgcg gcagccgcac gatgacatcc
    16921 ggctgcatcc gcgagcgggc gtcattttcg atgctgacct gggtttcata ttcataccct
    16981 tcacgcagac cggaagcctc cagcacccgc gtcaatacta cctcgcccca gttgccctgg
    17041 gttttattgt cgcctttcag cgcgcgcgtc aggttgatcg cttcctgggc catttgcgcg
    17101 ttgagttgct ggagattgcg aatttcgtgg gtcagggtat ggcgttcttg tgcttcttta
    17161 ccgaagctgt cctgaacctg acggcggaaa ccgtccagtt gttcacgtag cggcgacaac
    17221 aggctgttca gactctgacg gttttgctca tcaacccggc gattgctgtg ctcaaaaata
    17281 cggttggcga ggttttcaaa ctgctcactg aggcgctgct cgctgttaat catctggcga
    17341 attttatcgt cagcatgttg ctgtgcggct tccatccgcg tggttacttc acgcagatcg
    17401 gcctccagag aggtgttaat actttgcagg ctgcgcactt cgttattgag taactcgcac
    17461 tctgcacgcc agtgctcgct ttgggtaatt tgttgttttg ccgcgcttaa ctccgcgacc
    17521 atctcttcac gttcagctaa ttgctcggct ttttgctgcg catgttgata actggcaaac
    17581 agccagccaa ttgccacacc caccaacgca ataactgcgt aaaccatgat tgagaaatcc
    17641 acaacgcctc ctgacgcaac aacttacccg cacaaaatag aattgtgctg tacaaacgtc
    17701 cagttggaaa ggcatttcct gcgaggcgct acgcaaaaat acaaaaggcc gaacgcgtcg
    17761 gccttcagac aggagaagag aattacagca gacgacgcgc cgcttccacc acgattttca
    17821 ccgcttggct ttcagtttgt ttcatcgtct cagcattcgg gatctcttgc tgggtgcggt
    17881 taacgataac acccgctacc ataccggcac gcaggccctg acttgcacac atggtcagca
    17941 gggttgcaga ttccatttca tagttcatta cgcccatcgc ctgccactct tccatagaac
    18001 ctttaaagtg acgaactacg cgaccagagt aagtatcgta acgttcctgg cctgggtaga
    18061 aggtatcaga agaagctgtc acgccaacgt gagttgtcgc gccaatggat ttcgcagctt
    18121 caaccagcgc agtcgtacat tcgaaatcag cgacagccgg gaattccagc ggtgcgaagt
    18181 gcaggctcgc gccatccaga cggacagacg ccgtggtaac caggacatca cccacattaa
    18241 tatgcggctg aatagcgccc gttgtaccga tacgcaggaa ggtgcgaatg cccagctgtg
    18301 ccagctcttc aacagcaata gaggtagacg ggccgccgat accggtagag cagacgataa
    18361 caggtttacc atccagctct gcacgccagg tggtgaattc gcggtgagat gccagcttaa
    18421 ccggcttatc catcagcgcg gcgatctttt ccacacgatc cgggtcgcca gggacgatgg
    18481 caagcgtagc cccttgtaaa tcgtttttag tgaggccgag atgaaaaaca tcagacttgg
    18541 acatatacaa ctcctctgtg aatcggttta gtcagaagaa gcaaaaagac actttaccga
    18601 agggtttaac attttttcgt gatactcatc accatgacgc aaatgcgttg cataaattta
    18661 cccaccaaaa gtgatgcaaa tcacataaat cactcttcat tctgcaggac tgcataaaag
    18721 atgagccgat tcaggcaatg tgatttgttg catgctgact atagttaaca ttgcgcaaca
    18781 tttttataag ggtacggaga ataccatggc aacaacacaa caatctggat ttgcacctgc
    18841 tgcatcgcct ctcgcttcga ccatcgttca gaccccggac gacgcgattg tggcgggctt
    18901 cacctctatc ccttcacaag gggataacat gcctgcttac catgccagac caaagcaaag
    18961 cgatggccca ctgccagtgg tcattgtagt gcaggaaatt tttggcatgc atgaacatat
    19021 ccgcgacatt tgtcgccgtc tggcgctgga ggggtatctg gctatcgcac ctgaacttta
    19081 cttccgcgaa ggcgatccga atgattttgc cgatatcccc acgttgctta gcggtctggt
    19141 agcaaaagtg cctgactcgc aggtgctggc cgatctcgat catgtcgcca gttgggcttc
    19201 ccgcaacggc ggcgatgttc atcgtttaat gatcaccgga ttctgctggg gtggacgtat
    19261 cacctggctg tatgccgcgc ataatccaca gctaaaagcc gcagtggcgt ggtacggcaa
    19321 actgacgggc gacaagtcgc taaattcacc gaaacaacct gttgatatcg caaccgatct
    19381 taacgcgccg gttctcggct tatatggtgg tcaggataac agcattccgc aggagagcgt
    19441 tgaaaccatg cgccaggcgc tgcgggctgc taatgcgaaa gcagagatta tcgtgtaccc
    19501 ggacgccggg catgcattca acgctgatta tcgcccgagc tatcatgccg aatctgcgaa
    19561 agatggctgg cagcgtatgc tggaatggtt taagcagtat ggtgggaagc ggagttagtt
    19621 tcctcttagt ctatatttga gatggtgagt gaacgagtgc gttttgctgc catctctgtc
    19681 ccccaaaaaa taatcagtat gaattgattg gagattacga ctccgctatt tgaccttaag
    19741 aaagtcggca agtcatagtc ataaatgagt ctcttcaatg gaagacacct tagagattta
    19801 ccatctactc ccattcacaa acaaacatta attattatca acacattcac attaattata
    19861 aatcatctca gaccttcctg ataaaaagat attaaagtga aacattttgt ttttcacatt
    19921 ttagttcatc acttttaggc gtaaataaag gtaaaaaaaa caacaatact atttctcttt
    19981 cacaaaagaa ttaacaaaag gttttttaag gatgagtgat attatttact tgaccataac
    20041 cggagaacaa caaggatgta tttcctctct ttgtggaacg agcgcatcaa taggtaatcg
    20101 ctggcagatt ggccatgaag atgaaatctt cgcgttctcg ctctctaata gtattacgaa
    20161 tgcaggcaaa gggtcacaat tgcatggcct tagtttctgt aaattcttat taacgctatt
    20221 aacaataacg agcagttgtt catggcgttt gacttctatc ggatcaatag atttggtcga
    20281 tgggagaaat attacaacat tcaactccgc ggcgcgctct tatcagcaat caatcacctg
    20341 tttactgaga acaatctgga tacagaagcc atcactgtca gttatgaata tatactctcc
    20401 aggcatctga ttgctaatac tgaatttagt tatttggcat ttcccgataa ctacaaccgt
    20461 ctgtttatac cacggccaaa aactaaagct ggtaatggtt caaaacactt aatagtaaag
    20521 cggttggcag actgcttgct gctggcggca tatacaatgg gaatatcgaa ggattcaggg
    20581 aaacagcaga caagttgggg ggagatgcac ctgctggtta cgatcaattt atggataaca
    20641 aagggttact catcgctggt gcttcaatag ttgctggagc agttctgggg agaatgagac
    20701 tccctgattt ggaatcactt gagcatttcg gagccagagg agcagtatca ggaa
//
LOCUS       DAJTOR010000061        20688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTOR010000061 DAJTOR010000000
VERSION     DAJTOR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.061"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..214)
                     /gene="hyfA"
                     /locus_tag="OO249_001987"
     CDS             complement(<1..214)
                     /gene="hyfA"
                     /locus_tag="OO249_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5636300.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQR"
     gene            complement(467..937)
                     /gene="bcp"
                     /locus_tag="OO249_001988"
     CDS             complement(467..937)
                     /gene="bcp"
                     /locus_tag="OO249_001988"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5636301.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(937..1509)
                     /locus_tag="OO249_001989"
     CDS             complement(937..1509)
                     /locus_tag="OO249_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5636302.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1655..2533
                     /gene="dapA"
                     /locus_tag="OO249_001990"
     CDS             1655..2533
                     /gene="dapA"
                     /locus_tag="OO249_001990"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5636303.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            2550..3584
                     /gene="bamC"
                     /locus_tag="OO249_001991"
     CDS             2550..3584
                     /gene="bamC"
                     /locus_tag="OO249_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5636304.1"
                     /translation="MAYSVQKSRQAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            3797..4510
                     /gene="purC"
                     /locus_tag="OO249_001992"
     CDS             3797..4510
                     /gene="purC"
                     /locus_tag="OO249_001992"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5636305.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKRFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            4678..5541
                     /locus_tag="OO249_001993"
     CDS             4678..5541
                     /locus_tag="OO249_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5636306.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            5556..7571
                     /gene="tmcA"
                     /locus_tag="OO249_001994"
     CDS             5556..7571
                     /gene="tmcA"
                     /locus_tag="OO249_001994"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5636307.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            7645..8343
                     /gene="ypfH"
                     /locus_tag="OO249_001995"
     CDS             7645..8343
                     /gene="ypfH"
                     /locus_tag="OO249_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5636308.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFLGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(8453..8653)
                     /locus_tag="OO249_001996"
     CDS             complement(8453..8653)
                     /locus_tag="OO249_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5636309.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(8681..9808)
                     /gene="dapE"
                     /locus_tag="OO249_001997"
     CDS             complement(8681..9808)
                     /gene="dapE"
                     /locus_tag="OO249_001997"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5636310.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(9812..10168)
                     /locus_tag="OO249_001998"
     CDS             complement(9812..10168)
                     /locus_tag="OO249_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5636311.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            10549..10608
                     /gene="ypfM"
                     /locus_tag="OO249_001999"
     CDS             10549..10608
                     /gene="ypfM"
                     /locus_tag="OO249_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5636312.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(10707..13819)
                     /gene="acrD"
                     /locus_tag="OO249_002000"
                     /pseudo
     CDS             complement(10707..13819)
                     /gene="acrD"
                     /locus_tag="OO249_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(13982..15682)
                     /gene="narQ"
                     /locus_tag="OO249_002001"
     CDS             complement(13982..15682)
                     /gene="narQ"
                     /locus_tag="OO249_002001"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR5636313.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQHLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            15888..17867
                     /gene="aegA"
                     /locus_tag="OO249_002002"
                     /pseudo
     CDS             15888..17867
                     /gene="aegA"
                     /locus_tag="OO249_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
     gene            17935..18510
                     /gene="nudK"
                     /locus_tag="OO249_002003"
     CDS             17935..18510
                     /gene="nudK"
                     /locus_tag="OO249_002003"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5636314.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            18636..19679
                     /locus_tag="OO249_002004"
     CDS             18636..19679
                     /locus_tag="OO249_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5636315.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(19789..20688)
                     /locus_tag="OO249_002005"
                     /pseudo
     CDS             complement(19789..20688)
                     /locus_tag="OO249_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
BASE COUNT         4999 a         5292 c         5538 g         4859 t
ORIGIN      
        1 ccctctggga gatggcattg accgggcaga cctgcaggca aggggcttcc tcacagtgat
       61 gacacacgac aggggcagtg attgttgacg tcttcgccag ggccaggcgc gggtgttgct
      121 gtaaaccttg cgttttatgc acgtccgaac aggcagcgag acaggtatta catcctgtac
      181 accacagtgg ttcggccacc acaaagcggt tcatgtcggc tccttgaaaa tgatatgaaa
      241 cattgcattt catattgcat aaaggatgcc agtctgatta atttaatata ttcaataaat
      301 tatgtattaa cagggttctg aacggtacaa aaatgatggt cattaacgtt cagcaaaaaa
      361 tggattcgac acccgtgtcg caggggaaag cgagaatgtg atcttgatca cgagaagtga
      421 ggtgtgctga ccgcaagcgc agccagcacg gaatggagca aagtaatcag gcgtgttctt
      481 tcagccagtt cagcacaacg tcgtggtgat tgctggtttt aaaatcgtca aagacatgtt
      541 cgattttgcc atcagcgtca atcaggaagc tgatgcgatg aataccatcg taggttttgc
      601 ccatgaagga cttttcaccc cagacgccga attgttcgca cacctggtgg tcctcatcag
      661 acaggagcgt aaagttaagc agctcttttt ccgcaaaacg ggagagtttt tcgggtttat
      721 cggtgctgat acccagcaca tcaacgcccg cttttttcaa ctcatccatg ttatcgcgta
      781 agccgcaggc ctgtacggta cagccggggg tcatggcttt cgggtagaaa taaaccagaa
      841 cacgctgtcc ctggaagtcg gtcaaattaa cttgttctcc gtcttgatcc ggcaagctaa
      901 atttcggtgc gatatcaccg gctttcagtg gattcattac ttaactccat cctgttcatc
      961 atgttgggaa taattgacga cgttaatact gccttgtgca ttgagttctg tacatagggc
     1021 tttgaacgct tgctcaatat ttgctgcgtc cgcagatgcg gggctgtggg cggtgatctg
     1081 aatatgcaac tgcgcagccc gttcattttc agcaggttgc gtgcgcgaca ccagctccgc
     1141 aatgttcata tgatgcgcgt cgaaaagtgc tgtgaagcgt tcaattaaat gcggggagtc
     1201 tgcaacatcg acctgaaccc agacagatgc tggcattggc ggacgcggac gcgccgtcgt
     1261 gcgcttcatc acgattaaaa gatccagttc ggcacctttc aacggtaacg ttgattcaat
     1321 cagagtaatg gcattccatg aaccggaaag cagcatgata aacgtgaact cttctcccag
     1381 catcgccagg cgactgtctt caatattaca gccgcaacta ctgacatgac gggtgatggt
     1441 gttcacaatt ccagggcgat cggcacccaa cgcagtgatc accagataat gttgcgatga
     1501 cagtgtcaaa ctggttattc ctttaagggg tgagttgttc ttaaggaaag cataaaaaaa
     1561 acatgcatac aacaatcaga acggttctgt ctgcttgctt ttaatgccat accaaacgta
     1621 ccattgagac acttgtttgc acagaggatg gcccatgttc acgggaagta ttgtcgcgat
     1681 tgttactccg atggatgaaa aaggtaatgt ctgtcgggct agcttgaaaa aactgattga
     1741 ttatcatgtc gccagcggta cttcggcgat cgtttctgtt ggcaccactg gcgagtccgc
     1801 taccttaaat catgacgaac atgctgatgt ggtgatgatg acgctggagc tggctgacgg
     1861 gcgcattccg gtgattgccg ggaccggtgc taacgctact gcggaagcca ttagcctgac
     1921 gcagcgcttc aatgacagcg gtatcgtcgg ctgcctgacg gtaacccctt actacaatcg
     1981 tccgtcgcaa gaaggtttgt atcagcattt caaagccatc gctgaacata ctgacctgcc
     2041 gcaaattctg tataatgtgc cgtcccgtac tggctgcgat ctgctcccgg aaacggtggg
     2101 ccgtctggcg aaagtaaaaa atattatcgg aatcaaagag gcaacaggga acttaacgcg
     2161 tgtaaaccag atcaaagagc tggtttcaga tgattttgtt ctgctgagcg gcgatgatgc
     2221 gagcgcgctg gacttcatgc aattaggcgg tcatggggtt atttctgtta cggctaacgt
     2281 cgcagcgcgt gatatggccc agatgtgcaa actggcagca gaagggcatt ttgccgaggc
     2341 acgcgttatt aatcagcgtc tgatgccatt acacaacaaa ctatttgtcg aacccaatcc
     2401 aatcccggtg aaatgggcat gtaaggaact gggtcttgtg gcgaccgata cgctgcgcct
     2461 gccaatgaca ccaatcaccg acagtggtcg tgagacggtc agagcggcgc ttaagcatgc
     2521 cggtttgctg taaagtttag ggagatttga tggcttactc tgttcaaaag tcgcgccagg
     2581 caaaggttgc gggtgtttcg cttattttat tactcgctgc ctgtagttct gactcacgct
     2641 ataagcgtca ggtcagtggt gatgaagcct acctggaagc ggcaccgctt gcggagcttc
     2701 atgccccggc tggaatgatt ttgccggtga cctccggtga ttatgcaatc ccggtgacca
     2761 acggtagtgg tgctgtcggt aaggcgctgg acattcgtcc accagcccag ccgctggcac
     2821 tggtttctgg cgtgcgtacc cagttcacgg gcgataccgc ttcattgctg gtggaaaatg
     2881 gtcgtggcaa tactctgtgg ccgcaggtgg ttagcgtgct gcaggcgaaa aactacacca
     2941 tcacccaacg tgatgatgct ggtcagacac tgaccatcga ttgggtacaa tggaaccgtc
     3001 tggacgaaga cgagcagtat cgtggtcgtt atcaaatctc tgttaagccg cagggttatc
     3061 agcaggcggt tacggttaaa ctgctgaacc tggaacaggc gggcaaaccg gttgcagacg
     3121 cggcttccat gcagcgttac agcacggaga tgatgaacgt tatttccgcc ggtctggata
     3181 aatctgcctc tgacgccgcg aacgctgcgc aaaatcgtgc ctccaccact atggacgtac
     3241 aaagtgcagc tgatgacacc ggtttaccga tgctggtcgt acgcgggccg ttcaatgtgg
     3301 tttggcaacg tctgccagcg gcgctggaaa aagtgggcat gaaagtgacc gacagcaccc
     3361 gttcgcaggg taacatggcc gtaacttata agccgctgtc tgacagcgac tggcaggaac
     3421 tgggcgcgag cgatccaggc ctggcatccg gtgactataa actgcaggtt ggcgatttag
     3481 ataaccgcag cagcctacag ttcatcgatc cgaaaggtca tactctgact cagagtcaga
     3541 acgacgcgct ggtagctgtc ttccaggctg cgtttagcaa gtaaaaatac agggctggag
     3601 tcatccggcc cttttttctg atatgatacg caaacgtgtg cgtcggcaga aaaatgcaat
     3661 tttagcggta attcgcacga aatttgtttg tcggacgtag gtcggataag gcgttcttgc
     3721 cgcatccgac aaaacatccg gcacaccaga cagcaaaaga ttttaaaacg ttaattcaca
     3781 cccaggagtg ataaagatgc aaaagcaagc tgagttgtat cgtggtaaag cgaaaaccgt
     3841 atacagcacg gaaaacccgg acctgttggt gctcgaattc cgcaatgata cgtcagcagg
     3901 ggatggcgcg cgcattgagc agtttgatcg caaaggtatg gtgaacaaca agttcaacta
     3961 cttcattatg agcaaactgg ctgaagcggg tatcccgact caaatggagc gtctgctctc
     4021 cgataccgaa tgtctggtga aaaagctgga tatggtgccg gttgagtgtg tcgtgcgtaa
     4081 ccgtgctgct ggctctctgg tgaaacgtct tggaatcgaa gaaggtattg agctgaaccc
     4141 gccgctgttc gatctgttcc tgaaaaacga tgccatgcac gatccgatgg tcaacgaatc
     4201 ttactgcgaa acctttggct gggtgagcaa agagaacctg gcgcgtatga aagagctgac
     4261 ctacaaagcg aacgacgtgc tgaaaaaacg gttcgatgat gctggtctga ttctggtcga
     4321 cttcaagctg gagtttggtc tgtacaaagg tgaagtggta ctgggcgatg agttctcccc
     4381 ggacggcagc cgcctgtggg acaaagaaac actggagaaa atggacaaag accgtttccg
     4441 ccagagcctc ggtggcctga tcgaagccta tgaagccgtc gcccgccgcc tgggtgtaca
     4501 gctggactga tttttctgtt catcatcttg ccgtgcagct ggcacggcaa gacaaccgct
     4561 ctcggaatat atgcattgaa cgctcattcc tccgccattt catcccgctt tcctgtggta
     4621 atcttcctct gaaccaccta cgatcatttc tataatgaat atattgttga ggtatctatg
     4681 cgttggcaag ggcgacgtga aagtgacaat gttgaagaca ggcgcaacag ctccggtggt
     4741 ccgtctatgg gcggtcccgg ttttcgcctg ccaagcggta aaggcgggct gattttactg
     4801 atagtcgtgc tggttgcagg ctactatggt gttgatttaa ccgggttaat gactggacag
     4861 ccggtttccc aacaacaatc aacgcggtcc attagcccta atgaagacga agctgcaaaa
     4921 ttcacctcgg tgattctggc aaccacggaa gacacctggg gacaacagtt cgagaagatg
     4981 ggtaagacct atcagcaacc gaagctggtc atgtaccgtg gaatgacgcg caccggctgc
     5041 ggggcgggcc agtccattat ggggccgttc tattgcccgg cggatggcac agtttatatc
     5101 gatctctcct tctatgatga catgaaagac aaacttggcg cggatggcga ttttgcccag
     5161 gggtacgtta tcgcccatga agtcggtcat catgtgcaga aactgttagg catcgagccg
     5221 aaagttcgtc aactgcaaca aaacgcgacg caggcggaag tgaaccgctt atctgtgcgt
     5281 atggaactcc aggccgactg ttttgccggt gtctgggggc ataacatgca gcagcaaggc
     5341 gttctggaaa ccggcgatct ggaagaggcg ctgaatgcgg cgcaggccat cggcgatgac
     5401 cgtttacaac agcaaagtca ggggcgagta gtaccagaca gtttcacaca tggcacttct
     5461 cagcaacgct acagctggtt taaacgtggt ttcgacagcg gcgatccggc acaatgcaat
     5521 acttttggta aaagcattta atttccgggg cagggatggc tgaactgact gcgctttaca
     5581 cattaacagc gcaaatgaaa cgtgaaggga tccgccgctt gctggtgttg agcggggaag
     5641 agggttggtg ttttgatcat gcgcttaagt tgcgtgatgc cttacttggc gactggctgt
     5701 ggatttcgcc gcagccagat gctgaaaacc actgttctcc ctcggcgcta caaactttac
     5761 ttgggcgcga gttccggcat gcggtattcg acgcccgcca cggctttgat gccgctgcct
     5821 ttgcggcact tagcggaacg ttgaaagcgg gaagctggct ggttttgtta ctccctgtat
     5881 gggaagagtg ggaaaaccaa cctgatgccg actcgctgcg ctggagtgat tgccctgacc
     5941 ctattgcgac gccgcatttt gtccagcatc tcaaacgcgt acttacggcg gataacgacg
     6001 ctattctctg gcggcaaaac cagccgttct cgttggcgca ttttactccc cgtactgact
     6061 ggcaccccgc gaccggcgca ccacaaccag aacaacagca actcttacag cagctactga
     6121 ccatgccgcc gggcgtggca gcggtaacgg ctgcgcgtgg gcgcggtaag tcggcgctgg
     6181 cagggcaact catttctcgt attgcgggca gtgcgattgt caccgcgccc gcaaaagcgg
     6241 caacggatgt actggcacaa tttgcgggcg agaagtttcg ctttattgcg ccggatgcct
     6301 tgttagccag cgatgagcaa gccgactggc tggtggtcga tgaagccgca gccatacctg
     6361 cgccgttgtt gcatcaactg gtatcgcgtt ttcctcgaac gttgttaacc actacggtgc
     6421 agggctacga aggcaccgga cgtggttttt tgctgaaatt ttgcgctcgc tttccgcatt
     6481 tacaccgttt tgaactgcaa cagccgatcc gctgggcaca gggatgcccg ctggaaaaaa
     6541 tggttagtga ggcactggtt tttgacgatg aaaacttcac ccatacacca caaggcaata
     6601 tcgtcatttc cgcatttgaa caaacgttat ggcgaagcga gccagaaacg ccgttaaagg
     6661 tttatcagtt attgtctggt gcgcactacc ggacttcgcc gctggattta cgccggatga
     6721 tggatgcacc agggcaatat tttttacagg cggctggcga aaacgagatt gccggagcgc
     6781 tgtggctggt ggatgagggg ggattatctc aacaactcag tcaggcggta tgggcaggtt
     6841 ttcgtcgccc gcggggtaat ctggtggccc agtcgctggc ggcgcacggt agcaatccac
     6901 tggcagcgac attgcgtgga cggcgggtca gccggatagc agttcatccg gctcgtcagc
     6961 gcgaaggcgt tgggcaacag ctcattgcca gcgctttgca atatacgcaa gacctcgact
     7021 atctttcggt gagttttggt tacaccgggg agttatggcg tttctggcaa cgctgcggtt
     7081 ttgtgctggt acgaatgggt aatcatcgtg aagccagcag tggttgctat acggcgatgg
     7141 cgctgttacc gatgagtgat gcgggtaaac agctggctga acgtgagcat taccgtttac
     7201 gtcgcgatgc gcaagctctc gcgcagtgga atggcgaaac acttcctgtt gatccactaa
     7261 acgatatcgt gctttctgac gacgactggc ttgaactggc cggttttgct tttgctcatc
     7321 gtccgctatt aacgtcgtta ggttgcttat tgcgtctgct acaaaccagt gaactggcat
     7381 taccggcgct gcgtgggcgt ttacagaaaa atgccagtga tgcgcagtta tgtaccacac
     7441 ttaaacttgc aggccgcaag atgttactgg tccgtcagcg ggaagaggcc gcgcatgcgc
     7501 tgttcgcact taatgatgtt cgcactgagc gtctgcgcga tcgcataacg caatggcaat
     7561 tttttcactg actccttcaa ttttctccca tggtcattgt gagccagcgg cgtaaaatta
     7621 atgacatcag ttaaggagtt cgccatgaaa catgaccatt ttgttgttca aagcccggat
     7681 aaacctgtgc aacagttgtt actgcttttc ctcggtgtcg gggataactc ggtggcaatg
     7741 ggggaaatag gcagttggtt tgcaccgctg tttcctgatg cgctggtggt gagtgtcggt
     7801 ggcgcggagc cgagcggtaa tccggcgggg cgtcagtggt tttcggtgca gggtattacg
     7861 gaagataatc gccaggcgcg tgtggatgcc atcatgccga cgtttattga aacggtacgc
     7921 tactggcaga aacagagtgg agtaggggct aacgccacgg cgttaatcgg tttctctcaa
     7981 ggcgcgatta tggcgctgga gagtattaaa gccgaaccgg gccttgcttc acgcgtcatt
     8041 gcttttaacg gacgctatgc gagcctgccg gaaaccgcgt ctacagcaac gacaattcac
     8101 ctgattcatg gcggtgaaga tccggttatc gatctggcgc atgcggttgc ggcgcaggaa
     8161 gcgttaatca gtgccggtgg tgacgtgacg ctggatatcg tggaggattt aggccatgcg
     8221 atcgacaatc gcagtatgca atttgccctg gatcacttgc gttataccat tccgaaacat
     8281 tatttcgacg aagcgctaag tggcggtaag cctggcgatg atgatgtgat tgaaatgatg
     8341 taaaagacag attacctggc ctgataagac gcgtgagcgt cgcatcaggc atcgttcacc
     8401 attgccggat gcggcgttaa cgccttatcc ggcctacggt tgagcactca gcttacttct
     8461 tcggttgatc ctttttcggc cagtcgtcgt catcgtccca cttatcgttg aaatcacgat
     8521 gcgggggaag ctccggcttg ttggcgagaa atttcttgtg gtcgacgcgc ttgagatctt
     8581 taatcacgtt cagcaggacg cctaccaaaa agacaatcac cagaatccac caatatttag
     8641 ccagccagtc catgcttatt tcctcttgca gaaccactca tcaggcgacg agctgttcca
     8701 tgatacgttg atacatacgg gcaagtagct gcaggtcggc agcgttcaca cattcattaa
     8761 ttttatgaat agtggcattg accggcccga gttccaccac ctgcgccccc atgcgagcaa
     8821 taaagcgccc gtcggacgtt ccgcctgtgg tcagtagctg cggtttaatt tcattatagt
     8881 gctcaaccgc gttaacgacc gcatccacca gtttaccgcg cgcggtcaaa aatggctgcc
     8941 cggaaagcca ccaatccacc gtatagcgca gttgatgttt ttcaagcagg gcaagcacct
     9001 gcgctttgat catctcatca gtcagttcgg tgctgaagcg gaagttaaac tgcacaaaca
     9061 gttcacccgg aataacattg ttactgcccg ttcccgcctg aatattagca atctgcatac
     9121 tggtcgccgg gaaaaattca ttgccctgat cccactcaat agccactaat tcattaagga
     9181 aaggtgctgc gcgatgtact ggattgtcag ccagatgcgg gtaggccaca tgcccctgaa
     9241 cgccatgaat ggtaaggttg caggttaacg atccgcgacg accatttttc accacatcac
     9301 caaccacttc gatactcgac ggttcgccga ccaggcagta atcgagacgc tcattacgtg
     9361 ccatcaacgc ttcgacgact tttaccgtac cgttgtgggc actggcttct tcatcagagg
     9421 tgatcagaaa tgccagtcgc cccgtatggt tgggatgttg tgcgacaaag cgttctgccg
     9481 ctaccaccat cgccgccagc gagcctttca tatctgccgc accgcgcccg aataacatgc
     9541 catcacgaat ggtgggttca aacggcggat tgatccaacg atcggcgtcg ccaggcggca
     9601 ccacgtcggt atgcccggca aaggctaacg tttcaccttg cccacgccat gcccaaaaat
     9661 tctgcgtatc ggcaaagtcc atgcgttcaa cggtaaaacc gatcgcctgc aaacgttcaa
     9721 tcaacaaagc ctggcatcct gcgtcatcag gactcaggga agggcggcga ataagctgtt
     9781 gtgtcagctc aataaccggg cacgacatag actacacctc atggaaaaat tgctgataac
     9841 tggaatcact gaaacccagc agcataggct taccaggcgc gcagagcaat ggacgtttga
     9901 taattgaagg catttcagtc attaatgccg ccgcagaggc cgcatcggtg attttattgc
     9961 gggtggtttc gtccagttta cgccaggttg taccacgggt gttcagtaac gcttcccagc
    10021 ctaattcgtt gataaaaccg ttcaataatt cgctgtccag cccatcgacg cggtaatcat
    10081 gaaaacgata gtcgatgtta ttggcttcca gccaacggcg agccttttta atggtgtcac
    10141 aatttttgat gccgtaaagt gtaaccataa tgaatccttt ttcgatctat gagaataaat
    10201 actcatttaa gggtgaaaac attttgcctg caaatgatta tcaggaaata taacattatc
    10261 gaagtattca aattaacaac tattaaataa tattaaacat ttttaacgta aacattaaga
    10321 atattcatct tgttatctgt ctgtatttta accagaatta aatcagtatt agatgccgta
    10381 tgaatatctc gaaatgttgc tgtgtatcac atacaaatta tcaccgcagg accatagtat
    10441 ttacataagg tttgtccccc ggaggtagtc atcgcagcaa tcgagtaaat tagcttcacc
    10501 acggtatatt gtttccgtag aatacccata attataagag aggttgttat gattgaacgt
    10561 gaactgggga actggaaaga ctttatcgaa gttatgcttc gtaagtaatt cctgagacaa
    10621 agtacaaaca gcaagaaccc gctgagcagg ttcttaatcg gaaagttgaa aaaggcgaca
    10681 cattagcatg tcgccttttt tattgcttat tccgggcgcg gcttcagcgg gaagcggcgg
    10741 cgcaccagta caaagaacag cggcacgaag taaatagcca gaatagtggc cgaaatcatc
    10801 ccgcccatta cgccagtacc caccgcatgc tgaccaccgg aaccggcacc cgtgctggtt
    10861 gccattggca atacgccgaa gataaatgcc agcgaggtca tcagaatcgg gcgtaaacgc
    10921 tgacggcagg cgtggagcgt cgcttcaaac aggtcgtggc ctttttggtt catctcgtta
    10981 gcaaactcga cgatcaggat ggcgtttttc gccgataaac caatgaccgt taacaggccc
    11041 acctggaagt aaacgtcgtt ttccagcccg cgcatccagg ttgccagcag cgcgccgata
    11101 acccccagcg gcacgaccag cattaccgag aacggcaccg accagctttc atacaacgcc
    11161 gccagacaca ggaataccac cagcaaggaa atggcgtaca gcgccggagc ctgcgcgccg
    11221 gaaagccgct cctgatacga catcgccgtc cactccagac caaagccgtt tggcaactgc
    11281 ttcactaacg attccattat atccatcgcc gtaccggtac tgacccccgg tgcggcttcc
    11341 ccaacaatct caaccgcaga ataaccgtta tagcgttcca gacgcggcga gccagtctcc
    11401 cagcgtgagg tcgcgaaagc agagaagggc accatgccgc catctttatt tcggacatac
    11461 cagagattaa tgtcatctgg cagcatgcga tacggcgcag ctgcctgcac atagactttc
    11521 ttcacacgac cgcgatccat aaagtcattc acatagctcg aaccccaggc ggtttgcagt
    11581 gtgtcgttaa tatcgtcgat agcaacgccc agcgcctgag ctttacgctg gtcgatatca
    11641 atctgcaact gcggactgtc atcgaggccg ttatggcgca cacgggttag ctccgggttt
    11701 tccgccgcca gcgccagcaa ctggttacgt gctgccatca gcgcatcgtg acctgctcca
    11761 gcgtggtcct gcaactccat atcaaaaccc gcagaactac caagaccgct aattgctggc
    11821 gggctgctgg cgataacgcg agcttcttta atcttgttaa acgcctttgt tgcacgctcg
    11881 ataatggcaa acgaggtgcc ggtcttactg tcgcgttcgc tccagtcttt caggcggata
    11941 aacattcgcg ccacgttttg cccgttaccc ccagggccag aaccaacggt ggcaaacacc
    12001 gacatgatgt tgtctttttc atgggtgaag taatatttct cgatttgctc aacgactttc
    12061 agggtctgtt gttgggttga accgctgggc aattgtaccg aggtagtaaa catgccacgg
    12121 tcttccagcg gtaagaacga cgtcggcaaa cgcaggaaca ggaacaccat gccgccaagc
    12181 agcaggacat aaatcacaat ccagcgcagg ctgcggtgga gaattttcgc cacccctttt
    12241 tcgtagcgtt cggcgttgcg gttaaacatc tggttaaacc aggcaaaaaa gcctttttgc
    12301 ccgtgatgct cacctttttt cagtggctta agtagtgtgg cacacagagc cggagtgagg
    12361 atcatcgcta ccagtactga cagcaccatc gccgcaacaa tggtaataga gaactggcga
    12421 tagatggcac cggtggtgcc gccgaagaag gccattggta caaataccgc cgaaagcacc
    12481 atcgcaatcc cgaccagtgc cccctggatc tgccccatcg atttacgtgt ggcttcgcga
    12541 ggagcgagtc cttcctcact cataatacgt tcgacgtttt ccaccaccac gattgcgtca
    12601 tccaccagca gaccgatcgc cagtaccatc gcgaacatgg ttaaggtgtt gacgctgtaa
    12661 ccgaaggcgt aaagtacgga gaaggttccc atcaacacca ccggcacggc gatagtgggt
    12721 atcagcgtgg cgcggaagtt ttgcaggaac agatacataa cgaggaaaac cagggcgata
    12781 gcttccagca gcgttttcac cacgtcttca atggaggctt taacaaacga ggttgtttca
    12841 tacgccacct tgtattccag tccgtgcggg aagtactgcg ccagctcgtc gagacgattg
    12901 agcaccagct ccctgtcgcc atttcgtttg cgccggaggc cagttttacc cccagcccgg
    12961 aggctggctt accattgaag cggctaagat aatcgtattt ctccgccccc atttcgacgg
    13021 tggcgacatc gcccagcctt acctctgagc cgtcttgatt cacccgcaag gtgatatcgc
    13081 ggaactgttc tggtgtttgc agcagtgact gggcgttaat ggtggcgttg agcgcctgct
    13141 tatcgacgga aggtgtacca ccaagttgcc caaccgcaat ctgcgcgttc tgtgactcaa
    13201 tggcatcagt gacatcttta gccgtcatct ggaaactgtt gagtttcgcc ggggccagcc
    13261 agatacgcat ggaatattgc gaaccatagg catcgatatc cccgacgcca ttcacgcggc
    13321 ttaacgggtc ctgaatatta ctggcaacat aatcggcaat atcctgttta tccatcgaac
    13381 catcggtaga gacgaaagca atggtcagaa tattggtatc gccggtttta cgcaccgtca
    13441 cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc tgattgcagc tggttttgta
    13501 cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa acttaaagtg acagatgcct
    13561 gaccggtgcc actgctctga gatgacatat acatgagatt atcgaggccg gtcatatttt
    13621 gctcgataac ctggggcacg gtgttttcca gcgtctgggc cgatgcgccg ggatagttag
    13681 cggtcactcg cacattcggt ggcgcgagat cggggtattg ttcaacgggc aatgaaaaaa
    13741 tcgccagggt ccctgtcaga cacaacagga ttgccagcac ccaggcaaaa atggggcgat
    13801 caataaagaa attcgccatt aaaagaggac ctcgtgtttc tgcgtatcgt tgtaggcttg
    13861 acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg agaaaaaagg agttaatgta
    13921 aattatcatg cccttttata ctgaacctta agtgcaagta ttctttggtc agtaggaggc
    13981 attacatcaa ctgactttcc tcaccctctg cagagcgaaa gctaatactc actaacgtgc
    14041 cgccgccgga aggttgtgaa aaagtcagcg tcccacccag ccgttccgcg cgttcgcgca
    14101 tgatattcag accataatga ccttcgggtt ctttcggttc accgatacca atcccgttat
    14161 cacggatata aaccgtgtga ttgccgtccg gcgcggtgac gcaactgacg gcgatttcgc
    14221 tggcgttggc gtgcttcatc gcattcagca ccgcttcgcg aataatttgc aacaaatgca
    14281 cctgcatttg cgcatccagc gcaagggttg gcagacggca gtcgagggtc agtttggcgc
    14341 tggtttgatt ttgtaacgta tccagcattt ccctcaacgc ggaggggaga tccgcctgct
    14401 gcagcgtcag gcggaaagta gtcaacagct cgcgtaactg ccgataagca tcattcaacg
    14461 cctgggaaaa atcggccatg atactttgtg cggtggcgtt atcttccggt atcgaacgct
    14521 tcagtaacgt caactggata cgtaagtaag aaagtacctg agccagcgag tcgtgcaatt
    14581 cgcgggcgat ggttgcacgt tcttccatca acaataaatg ctgaaaatgc ttctgcgcct
    14641 gattaaagta caaaccgcgt cccagcatcg acgaaacgct gttaagcagc ggttctgatg
    14701 atgaaacgtg actattttgc cagtgcagtt cgccgtaaac cgtctcttgc attgtcaccg
    14761 gtaaaatctg catcggcaat tccgggtttg gttgcccttc gctaatccgc cagttttcac
    14821 cgacatttaa ctccagatat tcagccgctt cattgtcgcg aacaatctgc aaaatatggc
    14881 ggaaacaatg cacatcaatc tggcttgtgt tcagcgcctg cgaacattga tacaacacct
    14941 ccagacgacg cttggcctcg tggagatcgc gggtcttttc ttctactgac gcctccagcg
    15001 aaaggtacaa tttatgcagc tcgctcgaca tctggttaaa ggtttttgca agcagaccaa
    15061 gctcattcgg cagactggta tccagcggcg gcgagtcgaa ctgcccgtgt tcaatacgtt
    15121 gactggcggt aaccagctga ttcagcgggg caaccacctg atggcgtata cggcgcaaag
    15181 taaaaaagac cagcgtgaaa ataccgatac cgccagccag ggaaatcgcc accaccagca
    15241 gcattttgcg ttcagcgtag tgctgtaaag ccagcacgaa caggtctatc tgattaacat
    15301 aattattaat attggcctga taccacggca aatcgccctt gctgagccga ttattcatct
    15361 ccagccagtt ggcattcaga tgcgcatagc gagtttttac tgcttctggc acataccaga
    15421 cgttgagatt ggttaatacc ggtgaatgca gtgcctgctg aaacaactgg cgatgtgcat
    15481 tgagttgtgg actgccactt tgcaagtcgt agcccaggcg gtaactctgc atacgcagcg
    15541 atccggcaat attgatagcc tcagcgtcgc gcaaactgct cgccagagtt agcagagcga
    15601 tacccgtgga aagaatcgac agcagcacaa tgtaaaaaaa ggcccgggcc agactggccg
    15661 agacgggtcg tttaacaatc acacgcgtct tctccacaaa aaatcattaa tgttccagtt
    15721 cgctgaaacg caatttagtc agtgatggga ttatttgagc tgccacagtt tatagcgaaa
    15781 tagttactta ttctgggcac atgaacattg ccgattcccc tgtaagtcgg gtaataacaa
    15841 cactcatata aagaataagg tttttacaac caaaaaagaa ggtcgttatg aatcgtttta
    15901 ttatggccaa cagtcagcaa tgtctgggtt gtcatgcttg tgaaatcgcc tgtgtcatgg
    15961 cttacaatga tgagcaacat gtcctgagcc aacaccattt tcatccccga attacggtta
    16021 tcaaacatca gcagcaacgt agtgcagtga cctgtcacca ttgtgaagat gcgccctgcg
    16081 cccgtagctg ccctaatggc gcaatcagcc acgttgatga cagcattcag gtcaatcagc
    16141 aaaagtgtat tggctgtaaa tcctgcgtgg tggcctgtcc gtttggtacg atgcaaatcg
    16201 tcctgacacc cgtcgcggca ggaaaagtaa aagccacggc gcataaatgc gacctttgtg
    16261 cagggcgcga aaacggtcct gcctgtgtcg agaattgccc ggcggacgcg ctgcaactgg
    16321 tcactgacgt cgcactctcc ggcatggcga aatcccgccg cttgcgcacc gcacgtcagg
    16381 agcatcaacc gtggcatgcc agtaccgctg cacaagaaat gccggtaatg agtaaagtcg
    16441 aacaaatgca ggcaacgccc gcgcgtggcg agccggataa actggcgatt gaagcgcgca
    16501 aaaccggttt tgatgaaatt tatctgccat ttcgcgccga ccaggcacaa cgggaagcct
    16561 cgcgctgcct taagtgcggc gagcacagcg tttgtgaatg gacctgcccg ctgcataacc
    16621 atattccgca gtggattgaa ctggtgaaag ccggaaacat cgacgccgcc gtcgagcttt
    16681 ctcaccagac caacaccctg ccggaaatta ccggacgcgt ttgtccgcaa gaccgtttgt
    16741 gtgaaggtgc ctgtactatt cgcgatgagc acggcgcggt aactatcggc aacattgaac
    16801 gctatatttc agatcaggcg ttggcgaaag gttggcgtcc tgacttaagt catgtcacca
    16861 aagtggacaa gcgggtggcg attatcgggg caggtccggc agggctggcc tgtgcggatg
    16921 ttctgacccg caatggcgtg gcggtgacgg tgtacgatcg ccatccagaa atcggtggct
    16981 tgctcacttt cggcattcct tctttcaaac tggataaatc cctgctggca cgccgtcggg
    17041 aaatcttcag cgcgatgggg attcacttcg aactcaattg tgaagtgggt aaagatgtct
    17101 ctttggattc gcttttggaa caatacgacg cggtcttcgt tggcgtaggc acttaccgtt
    17161 ccatgaaagc gggtttaccc aatgaagatg cgccgggcgt ttatgacgcg ctgccgttcc
    17221 tcatcgccaa cactaaacag gtgatggggc tcgaagagct accggaagag ccgtttatca
    17281 ataccgccgg acttaacgtc gtggtactgg gcggcggcga caccgcgatg gactgtgtgc
    17341 gtactgcgct gcgccacggc gcgagtaacg tcacctgcgc ttatcgtcgt gatgaagcta
    17401 acatgccagg ctcgaagaaa gaggtgaaga acgcccgcga agagggggcc aacttcgaat
    17461 ttaacgtcca gccggtggcg cttgagctga atgaacaagg tcacgtttgc gggattcgtt
    17521 tcctgcgcac gcgtcttggc gagccggatg cctaggggcg tcggcgtcca gtaccggtgg
    17581 aaggcagtga atttgtcatg ccagccgacg cggtgattat ggcgtttggc ttcaatccgc
    17641 acgggatgcc gtggctggag tcgcacggtg taacggtaga caaatggggc cgcatcatcg
    17701 cggatgtgga aagccagtac cgttaccaga ccaccaatcc gaaaatcttc gccggtggcg
    17761 acgccgtgcg tggtgcggat ctggtggtta ccgcaatggc agaaggacgt catgcggcac
    17821 aggggattat tgactggctg ggggtaaaat cagtcaaatc tcactgatag cctgcgcaga
    17881 caaacccgac ttcacagcgt aagataattg ttcatttcgc gctgtggagt cggtatgacg
    17941 caacaaatca ccctcattaa agacaaaatt ctctccgata actatttcac cctgcacaac
    18001 attacctacg atctcacgcg caaagatggc gaagttatcc gccataaacg tgaagtttac
    18061 gatcgcggta atggtgcgac gatcctcctg tacaacgcga agaaaaagac cgtggttctg
    18121 attcgtcagt tccgtgtcgc tacctgggtt aatggcaatg aaagcgggca gctgattgaa
    18181 acctgcgccg ggctgctgga taacgacgaa ccggaagtgt gcattcgtaa agaagcgatt
    18241 gaagagacgg gctatgaagt gggcgaagtg cgcaaattat ttgaactgta tatgtcgcca
    18301 ggcggtgtga ctgagctaat ccactttttt atcgccgaat acagtgacaa tcagcgcgct
    18361 aacgccggtg gcggtgtcga agatgaagat attgaagtgc tcgagctgcc gttcagccag
    18421 gcgctggaga tgatcaaaac cggcgagata cgtgacggta agacggtgtt attgcttaac
    18481 tatttgcaaa cgtcacattt aatggactga aaaataacaa taatatttcg ttgtttatta
    18541 ttggctcaat ccgacaaatc cgattgagcc gcgctacctg cgcaacgaag atacgacttg
    18601 tgctgtttgt ttgaacttct ggggtcgtac cgtccatgcg ctatcgcatt ttccttctct
    18661 tttttttcgc tttgttgccg acgtctttgg tgtgggcggc accagcgcaa cgggcgtttt
    18721 ccgactggca ggtcacctgt aataaccaaa acttctgcgt ggcgcgtaat acgggcgatc
    18781 ataatggact ggtgatgacc ctgagccgca gcgccggggc gcataccgat gccgttttac
    18841 gtattgagcg cggcggattg aagtcgccgg aggcgtcaga aggggagata gcgccacggc
    18901 tgctgttaga tggcgagccg ttggcgttaa gtggtgacaa gtggcggatt tcaccatggt
    18961 tattagtaac cgatgatacg gcaaccatca ccgcgttttt gcagatgatt caggaaggga
    19021 aggcgatcac cttacgcgac ggcaatcaga ccatttcgct gagtggctta aaagcggcgt
    19081 tgttgtttat tgatgctcag caaaagcgcg ttggcagtga aaccgcgtgg atcaagaaag
    19141 gggacgaacc gccgctcagc gtaccgcccg cgcctgcgct gaaagaggtc gcggtggtta
    19201 acccaacgcc gacgccactc tcactcgaag aacgcaacga tttgctggat tatggaaact
    19261 ggcggatgaa tggtctgcgc tgctcgcttg atccattgcg tcgtgaggtg aatgtcactg
    19321 cgctgactga tgataaagcg ctgatgatga ttagctgtga ggcaggggcc tataacacca
    19381 ttgatttggc atggattgtg tcgcgtaaaa atccgctggc ctcgcgcccg gttcggttgc
    19441 gtttgccgtt caacaacggg caggagacga atgaactgga actgatgaac gcaacatttg
    19501 atgagaaatc gcgtgaactg gtgaccttag cgaaagggcg cggattaagc gattgtggca
    19561 ttcaggcgcg ctggcgcttt gacggtcagc gttttcgcct ggtgcgttac gccgcagaac
    19621 ccacctgcga taactggcat gggccagacg cctggcccac gttgtggatc acccgttaaa
    19681 caaaatggcg gggacatgta ggccggataa gacgctgtag gccggataag acgcgtcaga
    19741 gtcgcatccg gcctacagcg tccggcaatc agcatccggc gatcaccatc aggcaccttt
    19801 cactcccagc accttatgcg cttttgccac aatattctcg gcggtaaagc caaagaacgg
    19861 gaacagctta tccgccggag cagattcccc ataacctgtc atcccaacaa tcgccccttt
    19921 caaaccaaca tacttgtacc agtaatcggc aatacctgct tccaccgcca cgcgagccgc
    19981 aacgttagaa ggcaacaccg actcccgata ttcctcatcc tgggcgtcga aaatatcagt
    20041 cgagggcagg gaaactacgc gtacattgcg accttctccc gctaatttct ctgccgcttg
    20101 cagggggatt tccatctctg aaccggtggc aatcaaaata atatctggct taccgccgct
    20161 gtctttcagc acataaccgc cacgggcaat ctcttttacc tgatccggcg tacgttccac
    20221 ctgggccaga ttctgccttg agaggatcag tgccgtcggt ccgttgtggc gctcaaccgc
    20281 cagcttccag cccaccgccg cttccacctg atcgcacggt cgccaggtgc tgaaatttgg
    20341 cgttaagcgc aggctggcca gttgctcaac agcctggtgc gtcggaccat cttcgcccag
    20401 gccaattgag tcgtgggtat aaaccataat ctgccgcgct ttcatcagtg ccgccatccc
    20461 cgcggcgtta cgggcgtatt caacaaacat caggaacgtg gcggtatacg gcacaaagcc
    20521 gccgtggtgc gcgatgccgt tggctatagc ggtcatgcca aattcacgca ccccgtagtg
    20581 aatgtagttg cccgccggat cttccttcag cgaaacagaa cctttccaga tggtcaggtt
    20641 gctggagcca gatccgccga accgccgagc aactcaggca gcatcggc
//
LOCUS       DAJTOR010000062        20661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTOR010000062 DAJTOR010000000
VERSION     DAJTOR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.062"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..878
                     /locus_tag="OO249_002006"
     CDS             <1..878
                     /locus_tag="OO249_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HCR5636316.1"
                     /translation="DQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQ
                     YGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKG
                     FSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTN
                     IINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAED
                     GGFTLEINGSEKELYLLSGQQFLKCPLSVEKKRASIRYSGDVTCSVVTYSQLPELKQP
                     KLRGNVQTAQREVAP"
     gene            875..1964
                     /locus_tag="OO249_002007"
                     /pseudo
     CDS             875..1964
                     /locus_tag="OO249_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
     gene            complement(2052..3350)
                     /gene="exuT"
                     /locus_tag="OO249_002008"
     CDS             complement(2052..3350)
                     /gene="exuT"
                     /locus_tag="OO249_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5636317.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3833..5245
                     /gene="uxaC"
                     /locus_tag="OO249_002009"
     CDS             3833..5245
                     /gene="uxaC"
                     /locus_tag="OO249_002009"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5636318.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5260..6747
                     /gene="uxaA"
                     /locus_tag="OO249_002010"
                     /pseudo
     CDS             5260..6747
                     /gene="uxaA"
                     /locus_tag="OO249_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6830..7381
                     /locus_tag="OO249_002011"
     CDS             6830..7381
                     /locus_tag="OO249_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5636319.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDDRRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7386..8630)
                     /gene="sstT"
                     /locus_tag="OO249_002012"
     CDS             complement(7386..8630)
                     /gene="sstT"
                     /locus_tag="OO249_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5636320.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9246..10211)
                     /gene="alx"
                     /locus_tag="OO249_002013"
     CDS             complement(9246..10211)
                     /gene="alx"
                     /locus_tag="OO249_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5636321.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINARVNYRHDKQRGE"
     gene            complement(10494..11480)
                     /locus_tag="OO249_002014"
     CDS             complement(10494..11480)
                     /locus_tag="OO249_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5636322.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(11559..12242)
                     /locus_tag="OO249_002015"
     CDS             complement(11559..12242)
                     /locus_tag="OO249_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5636323.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHSQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12319..12822)
                     /locus_tag="OO249_002016"
     CDS             complement(12319..12822)
                     /locus_tag="OO249_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5636324.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12907..14043
                     /gene="rlmG"
                     /locus_tag="OO249_002017"
     CDS             12907..14043
                     /gene="rlmG"
                     /locus_tag="OO249_002017"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5636325.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     STTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14328..14642
                     /gene="higB"
                     /locus_tag="OO249_002018"
     CDS             14328..14642
                     /gene="higB"
                     /locus_tag="OO249_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5636326.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14639..15055
                     /gene="higA"
                     /locus_tag="OO249_002019"
     CDS             14639..15055
                     /gene="higA"
                     /locus_tag="OO249_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5636327.1"
                     /translation="MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(15100..17118)
                     /gene="fadH"
                     /locus_tag="OO249_002020"
     CDS             complement(15100..17118)
                     /gene="fadH"
                     /locus_tag="OO249_002020"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5636328.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSESGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELNARRAIAQGTRLALEI"
     gene            complement(17544..19895)
                     /gene="ygjK"
                     /locus_tag="OO249_002021"
                     /pseudo
     CDS             complement(17544..19895)
                     /gene="ygjK"
                     /locus_tag="OO249_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19912..20661)
                     /gene="ygjJ"
                     /locus_tag="OO249_002022"
                     /pseudo
     CDS             complement(19912..20661)
                     /gene="ygjJ"
                     /locus_tag="OO249_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
BASE COUNT         5035 a         5416 c         5432 g         4778 t
ORIGIN      
        1 gcgatcaaac gtcatggcag ttatcgcata ccttttataa tgattcattc agccataaag
       61 aacttggcgt aaccgtcgga ggcctgaaca ccgataccat aaacagcgcg gttaacgggc
      121 gttgggatgg gcaatacgga aatgtctacg ctaccgtatc tgatagttat gaccgtaaga
      181 atcatgatca tctttcggcc tttacgggca cttatagctc tacgctggct gtcagtcgct
      241 atggcgttaa tttgggagcc agtggtacag acgatttgct gggtgcggta ttggtggatg
      301 tgaaaggctt ctctgaacag gatgaagaga gtcaggatct gcaactcgaa gcgcgggtgg
      361 caggcagccg aactttgcag cttggtcaaa gcgacagtgt gttgttccct tatcctggat
      421 ttcagtctgg ttttgttgag gttaacgaca gtagccaggg caatcagcag gggacaacaa
      481 acatcattaa cggtgcaggg aatcgtgaat taatgttgtt gcctggcaag ctgcgctatc
      541 gcgaagtgtc tgccagcttt aactacaact atatcggtcg tttattactg ccggcagcgg
      601 tgaaaaaatt cccgattgtg gggctgaaca gcgccatgtt actggtagct gaagatggcg
      661 gatttacact tgaaattaac ggcagcgaaa aagagctcta tctgctttcc gggcagcaat
      721 tccttaagtg tccactgagt gttgaaaaga aacgcgccag cattcgttac agcggagatg
      781 ttacttgtag tgtggtgact tattcacaat taccggagtt gaaacagcct aaattacgtg
      841 gaaacgttca gacggcgcaa agggaggttg caccatgaga aatcgattga ttgcggcgat
      901 attgggcttg tttggcacgc tcactggcgt tcaggcagct cctgacgtga ccagtgaaat
      961 tacgtatgat ttggcatctg gtagagcgga ttattacttc tggaaggatg aagcgtcggc
     1021 aggaaataat ggatatatgt ggtatgaatg ttcgtatcct gacctccaac aaacctgtac
     1081 agctaatgga aatatatcga cagtacaaat ctatttaact gaacaacgca gtgggatgcg
     1141 ttggccggta aaactcaaag gatttaaaac agccattgta agtagcgatg aagcgccgct
     1201 aggatgcaag ggggggcaaa gggcttcaga cgaatcttaa ggattctaat agatcttcat
     1261 gtacagaaga tggtcaacat tattatatat acgatacaaa gtttcttacg ctctaccttg
     1321 agcagacaga gatgaagaat ttgccgattg gtggcgtctg gaaggggaaa gttaaattac
     1381 attcgaacag cccggcccag gactatttcg caaatattac cctgaatacg ctcgacccca
     1441 accatattga cgtgttcttc ccggagttcg cccacgccac gccgagggtg cagttagact
     1501 tgcatccaac aggaagcgtt aacggcagca actacgcgca agatctgacc atgttggaca
     1561 tgtgcctgta cgatggtttt aacggtaatg ccatcagtta tgaaatcatg ctcaaagatg
     1621 aagggcgacc agctgcaggg cgcagagacg gtgacttctc tatctatcgt cagggaggaa
     1681 cctctaccga cgagggagaa cgcattgatt accgggtcaa aatgtacaac ccggaaaccg
     1741 gtgggcaaat tgatgtgcgc aataatgaaa atatggtctg gaacagcatt aacctgaaaa
     1801 gtgtgcgtcc ggtggtactg cccggtattc gctatgccgt gatgtgtgtg ccgacaccct
     1861 tggctctggc agtcgataaa ttcagcgtga tggataaaca ggccgggtac tacatgggca
     1921 aattgtcagt aatctttacg ccttccttgc caaccatcaa ttaaccctgc aaagcggaca
     1981 aacgcttttt tctatataaa cgacgaagcc gcctgataag gcggcttcgt cgttataact
     2041 tacgttaaac gttaatgttg cggtgcggga tcgttatgtg attcctgcgc tacctcaatc
     2101 gccggtttgt tctgcaacac ggtccagata accagcgcac ctaacaggtc gaacactgcc
     2161 agtactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgaa
     2221 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     2281 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgagca
     2341 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag gccgatcata
     2401 cccgggccaa tcatcagcac tgcacccagc gttacgacca tcttacggga aacgatcagg
     2461 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     2521 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     2581 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggctgg ttctgccagg
     2641 aaacgcggca gcgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     2701 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     2761 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     2821 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg cattacgatt
     2881 gcccatacca ccagcggcgg cgcaatcatc gcaccaatcg aagaacctac gttaaagtaa
     2941 cctaccgcga tggaacgctc tttcgccggg aaccattcgg agctggcttt cagacccgcc
     3001 gggatcatcg ctgcttccgc ggcaccgact gcaccacgag caatcgccag gccacccctg
     3061 ctaccagcca gcgcggttgc accacagaat acggcccaca gtacagcaaa cattgcataa
     3121 ccgattttcg tacccagcac atccagtaca tagcccgcta ccggttgcat gaccgtataa
     3181 gcagcagaat aggctgcgat gatataggaa tactgttggg tggagatgtt taactcttcc
     3241 atcagagttg gcgcagctgc cgccacagtg ttacgcgtca ggtaaccaag cacggtgcca
     3301 agcgtcacca gtgcgatcat ataccaacgt aaccctttaa ttttacgcat gtaaaacctc
     3361 atcgttttgt tatatccgca ggaagcgcgg ccatcatccc gccagaggct gaagatgtta
     3421 gttacgaccg ggtaaccgaa aaaaaaccgg cacgccccga acgttgccat gaaagtttcg
     3481 tgctaattcg gcttccgtac cggttaaccc gattcatcta acaaatgaat gaatcggcaa
     3541 tttgcattcc gtcgacttat gatttgcgac ggcagaaaga tgacttgtca tacaacttta
     3601 aaaggtgaga gccatcataa atgtgcgaat atttgtaggg acattacctg acgacagcga
     3661 ggccagtact ggcgcggcct gcagcgagat ttaccacttt gagagtaatt tttttaacta
     3721 cgtttattga tctaactcac gaaaatatct tcggactctg gaaattggtg tgataacttt
     3781 gtcagcatcg caccataagc aagctagctc actcgttgag aggaagacga aaatgactcc
     3841 gtttatgact gaagatttcc tgttagatac cgaatttgcc cgccgtctgt atcacgacta
     3901 cgcaaaagac cagccgattt tcgattacca ttgccatttg ccgccgcagc agattgcgga
     3961 agactatcgt tttaaaaacc tgtatgacat ctggctgaaa ggcgatcact acaaatggcg
     4021 cgctatgcgt accaacggtg tggccgagcg tctgtgtacc ggtgatgcgt ctgaccgtga
     4081 aaaatttgac gcctgggcgg cgactgttcc gcacactatc ggcaacccgt tataccactg
     4141 gacgcacctc gaactgcgtc gtccgtttgg tatcactggc aaattgcttt ctccgtcaac
     4201 tgccgatgaa atctggaacg aatgtaacga actgctggcg caggataact tctccgcgcg
     4261 cggcatcatg cagcagatga acgtgaaaat ggtcggcacc accgatgacc cgatcgattc
     4321 tctggagcat cacgcagaga tcgccaaaga cggctctttc accatcaaag tgctgccgag
     4381 ctggcgtccg gacaaagcct tcaacatcga acaggcgacc tttaacgact acatggcgaa
     4441 gctgggcgaa gtttccgata ccgacattcg ccgctttgct gacctgcaaa ctgccttgac
     4501 caaacgtctg gatcacttcg ccgctcacgg ctgtaaagtg tctgaccatg cgctggacgt
     4561 ggtgatgttt gctgaagcga acgaagcgga actggacagc attctggcgc gccgtctggc
     4621 tggcgaaacc ctgagcgagc acgaagtggc acagttcaaa actgcggtac tggtgttcct
     4681 tggtgccgaa tatgcacgtc gcggctgggt acagcagtac cacattggcg cgctgcgtaa
     4741 taacaacctg cgtcagttca aactgctggg gccggatgta ggctttgact ccatcaacga
     4801 ccgtccgatg gcagaagagt tgtctaagct gctgagcaag cagaacgaag aaaacctgct
     4861 gccgaaaacc attctgtact gcctgaaccc gcgcgataac gaagtgttgg gcaccatgat
     4921 cggtaacttc cagggcgaag gtatgccggg caaaatgcag ttcggttccg gctggtggtt
     4981 taacgatcag aaagacggta tggaacgtca gatgacccaa ctggcgcagc tcggtttgct
     5041 gagccgcttt gtcggtatgc tgactgacag ccgtagcttc ctgtcataca cccgtcacga
     5101 atacttccgc cgcattctgt gccagatgat cggtcgctgg gtggaagcgg gcgaagcacc
     5161 ggcggacatc aacctgctgg gcgagatggt gaaaaatatt tgctttaaca atgcgcgtga
     5221 ctacttcgcc attgaactga actaaggtct ggggttgata tgcaatacat caagatccat
     5281 gcgctggata acgtcgcggt cgctttagcg gatttggctg aaggcacaga agtcagtgtc
     5341 gataaccaga ctgttacgct gcgccaggat gttgctcgtg gacataaatt tgcgttaacg
     5401 gatatcgcaa aaggggccaa tgtcatcaaa tatggcctgc cgattggtta tgcattggcg
     5461 gatattgcgg cgggggaaca cgtccacgcc cacaatacgc gcacgaatct gagcgatctg
     5521 gatcagtatc gctatcaacc tgattttcag gatctgcctg cgcaagcggc agatcgtgaa
     5581 gtgcagatct atcgtcgcgc taacggcgat gtcggggtgc gtaacgagct gtggatcctg
     5641 ccaaccgtgg gctgtgtcaa cggcatcgcg cggcagatcc agaaccgttt tctgaaagag
     5701 accaacaacg ctgaaggtac cgacggcgtg ttcctcttca gccacaccta cggctgctca
     5761 cagctgggcg acgatcacat taatacccgc accatgctgc aaaacatggt gcgccacccg
     5821 aacgcaggcg cagtgctggt gattggtctg ggctgtgaaa acaactaggt tgccgcattc
     5881 cgtgaaacgc tgggcgatat cgatcctgaa cgcgttcatt tcatgatctg ccaacagcag
     5941 gatgatgaga tcgaagccgg gatcgagcat ttgcatcagc tgtataacgt gatgcgcaac
     6001 gataaacgcg agccaggcaa actcagcgaa ctgaagtttg gtctggagtg cggtggttct
     6061 gacggtcttt ctggtattac tgctaacccg atgctggggc gtttctctga ctacgtgatt
     6121 gctaacggcg gtactaccgt actgaccgaa gtgccggaga tgtttggcgc agagcagttg
     6181 ctgatggacc attgccgcga cgaagcaacg tttgaaaaac tggtcaccat ggtcaacgac
     6241 tttaaacagt actttattgc ccatgatcag ccgatctacg aaaacccatc accggggaac
     6301 aaagcgggcg gtatcaccac gctggaagac aaatcacttg gctgtaccca gaaagcgggt
     6361 tccagtgtcg tggttgacgt gctgcgttac ggcgagcgtc tgaaaacgcc agggctgaac
     6421 ttgttaagtg cgccgggtaa cgatgccgta gcgaccagcg ccctggcggg tgcgggctgc
     6481 catatggtgc tgttcagtac tggtcgcggc acgccatacg gtggatttgt gccgacggtg
     6541 aaaatcgcca ccaacagtga actggcggcg aagaaaaaac actggctcga ctttgacgcg
     6601 ggtcagctga tccacggtaa agcgatgccg cagttgctgg aagaatttat cgacaccatc
     6661 gttgagtttg ccaacggtaa acaaacctgc aacgagcgta acgacttccg cgaactggcg
     6721 atcttcaaaa gcggcgtaac gctataaagt cgaaatgagt tgttaataaa aaacggcgtt
     6781 tcataatgtg aagcgccgtt tgcttttcca tacctgataa ggatttatca tgaccgcgta
     6841 ttggctggcc cagggcgtgg gtttcatcgc ctttctgatt ggtatcacaa catttttcaa
     6901 tcgtgacgac cgtcgcttca aaaagcagct ttcggtctat agcgccgtta ttggcgtaca
     6961 tttttttctt ctgggcacct atcccgctgg tgccagtgcc atccttaatg ccattcgtac
     7021 attgattacc ttacgcacgc gcagcttatg ggtaatggcg atttttattg tgctgactgg
     7081 cggaattggc ctcgcgaagt tccatcatcc tgtcgaacta ttgccggtta tcggtacgat
     7141 tgtcagtacc agggcgctgt tccgctgtaa agggctcacc atgcgctgcg tgatgtggtt
     7201 ttcaacgtgt tgctgggtga ttcacaactt ctgggcgggg tcgataggcg gcacaatgat
     7261 tgagggtagt tttctgctta tgaatggctt gaatatcatt cgtttctggc ggatgcagaa
     7321 aaggggaatt gatccgttta aagtagagaa aacccctccc gccgtagacg aaaggggtta
     7381 aacaattaat tacgcagggc gctatttgcc agacgatcgt cttccgcctg gcaagctgcc
     7441 gcagtgaaca gcacgtcagt tgaagagttc agcgcggttt cgcaagagtc ctgcaatacg
     7501 ccgatgataa agccgacggc aaccacctgc atggcgatat cgttcgaaat accgaacata
     7561 ttacaggcca gcgggatcag cagcagagaa ccccccgcca cgccggatgc gccacaggca
     7621 cacagagaag ccaccacgct caacagcagc gccgtgggca gatcgaccgg gatacccagc
     7681 gtattaaccg cagccagcgt caacacggta atggtgattg ctgcgcccgc catattgatg
     7741 gtggcaccca gcggaataga aacggaatag gtatcgcgat ccagattcag cttttcacac
     7801 agtgccatat tcaccggaat gttcgccgca gagctgcggg tgaagaaggc atacacgccg
     7861 ctttcgcgca ggcacagcag caccagcggg aacgggttac gacgaatttt ccaccacacc
     7921 agcaatgggt taaccaccag cgccaccagt aacatacagc caaccagcac gaccagcagt
     7981 tgcgcgtagc cccacagtgt ggagaaaccg gtggttgcca gggtagaaga aaccagacca
     8041 aaaataccaa tcggtgcgaa gcgaatgacc agtttcacca taaaggtgac ggcattcgac
     8101 agatcgttga ccaggttttt ggtggtctcg ttaccgtgac gcagtgcgaa accgaggcca
     8161 attgcccaca ccaggatccc gatgtagtta cctttcagca gcgcgtcgat ggggttggaa
     8221 accatgctca ttaccagccc gcgcatcact tctacaatgc ctgacggtgg cgaaatatca
     8281 cccgcgctac tggataagtg cagggtagaa gggaaggcga agctgaaaac gactgcggcc
     8341 agagcagcag agaaggtacc cagcagatag aggaacaaaa tagggcggat attggttttc
     8401 tgcccgtgct ggtggttagc aatagatgcc atcaccagca tcaacaccag gatgggggca
     8461 acggctttca gtgcgccgac gaacaaagta cctaacagac caacagcttc cgccgcgggt
     8521 tttgagatcc atgccagaag aatccccaga acaaggccga ccaggatttg ttttaccagg
     8581 ctgccatgag ccagacgccg gaatagcccc ggtgaacgtt gcgtagtcat ttttcgatcc
     8641 tttcattgtg ttgtcgcaca tccctggtgc gttctgtcga cgcatctttc aggatgtaac
     8701 aaagtgtttg caaggtcgag tataaggaaa gttacagctt cgggaagcga aaaatgctgg
     8761 attttaaaga gaggtcatat tttgcaactt aattgttaca tttgtttgac ataaacagag
     8821 ggaaggactg gctactaagg ctgattagat aacatatggg caaaactatt tggaatatga
     8881 gaacggttgc gctcctttaa tgtgctatgc catataaagt cactggcgca gggagcgcgc
     8941 agggggcagc caatcgccgc cgccccctgc acccccaggc tctggcgaac aaaatcgccg
     9001 ctgcgcgatt ccctcagctt atcccttgcg gctaccgggt cgggcgcaag ggaacgtccc
     9061 tgtaagatgc gccctcgccg cgcatccatg cgcgtcgccc cggcctccag ggaacgcttc
     9121 ggcgattttg acgccaccaa cacccgtgcg gtctttttgt aaagagctac aagccaatta
     9181 cataattaat agctcacctt tgtctgggat gagttatctc taggaatggc ttaatataca
     9241 gcgaatcatt ctccccgctg cttatcatgc cgataattca ccctggcgtt gataataaac
     9301 gtcatcacca gaatgccaaa caccacgccc agcgagacgg cgattggaat atggtagaag
     9361 tcgacaatca gcatcttgat accgataaac accaggatca ccgccaggcc atatttgagc
     9421 atcgagaaac gctctgctac gcccgccagc aggaaataca tcgcacgcag gccgaggatc
     9481 gcaaacaggt ttgaggtcag cacaatgaac gggtcagtgg tcacggcgaa gatagccgga
     9541 atgctatcca cggcgaaaat cacgtcgctc aattccacca gaatcagtac cagcatcagc
     9601 ggtgtggcgt acagcaatcc attcttacgc acaaagaaat gctcgttgtc gatggtgtcg
     9661 gtcatgcgca aatgaccgcg tagccagcgc accagcggct tgtcgccaat gcctgattca
     9721 tcttcatggg cgagcgccat cttcacgccg gtaaacagca ggaaggcacc gaagatatac
     9781 aggatccagt cgaactgcga aattagccag ctgccagtga agatcatgat ggtacgcaga
     9841 acaatcgccc cgagcacgcc atacaccagc acgcggcgtt gtaatgccgc cggaacagag
     9901 aaatagctga acaacatcag ccagacaaag acgttatcga ccgccagcga tttctcaatc
     9961 agataacccg tgagaaaggc cagtgcctgt ggatcggcaa cggcgcgacc ttcggtttgc
    10021 accagatacc accagaaagc ggcgttaaac agtaacgaca gcgtcaccca aaccagcgac
    10081 caggccgcag cctgtttcat ggtcatggca tgtgccccac gacgcccctg caacaacagg
    10141 tcgatagcca gcataatggc gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg
    10201 acagtattca tagaagttcc ttacacataa aaaaaacggc caacgtcgga agacgtcagc
    10261 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    10321 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cacacatcgt
    10381 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagcca
    10441 ttcagccagt caatggtctg ttacatacct tttacactgt tttactttgg caattatagt
    10501 tttacgctat ctgccggaaa aatcacccca gtctggcggc ggatctcagt cagcagtttg
    10561 gcggtgatgc gactgaccgc cagccctgga tgatccacca gatgctcatc caccagggta
    10621 gcaaacagct ctgcttcgta gagcatggta ttaatatgct gcggctgggt gagatcctgc
    10681 atctggctgc cacgcggcac gaagcacact ttctggcatt cagacagttt ttcaatcacc
    10741 agcgatcccg cttcgccctg aatctcgctt tccaggacag aatcactgac ctttgagtgc
    10801 agcaacgtaa cgctgaaatc accgtaatcc atcaccacca cgccgtgggc atctacgccg
    10861 cttgccaaca aactggcggt tgcctgcacg cttttcggtt caccgaataa tgccaccgcc
    10921 gacgccaggc agtaaaagcc gatatccata attgaaccgt tagagaatgc cggattaaag
    10981 gtgttgggat tctcaccatc caggtaacgt tgataccgcg aggaatactg gcaatagttg
    11041 aaaaagactt tacgcaattt gccgactttc ggcagcgcct ggcgcagcaa atgaaagttc
    11101 ggcaggctgg cggttttaaa tgcctcaaac agcaccacct gattttcccg cgcacaggca
    11161 atggcggcat ccacttccgc cagattcgac gccagtggtt tctcgcaaat cacatgaatt
    11221 ttatggctaa ggaaaagttg tgtctgggaa aagtgcaggg cattcgggct ggcaatatac
    11281 accgcgtcaa tggcatcgct ttccgccatc gcttccagcg aggtaaacag atgctcgaca
    11341 gaaaaatcat tggcgaagtg ctgggcctgt tcaaggctgc gggaatatac ggcggttaac
    11401 ttgtatttac cgctctcatg ggcggcctcg acgaactggc gagtgatcca gttcgtacca
    11461 atcacagcga aacgtatcat aacgcgtgga tactccataa agggattctg tcgtcaatgt
    11521 agcacgcctt tttcatcttc gacgtgcgca gatacgcatt attcttttgc cggacagcca
    11581 tgctcactaa acggcccaat catgaccgat ggcccgagat atttcggctg gctatggagg
    11641 attttggcgt caatcacggc gctgacacga gcgagtttct ctcgcagttg agtataaaaa
    11701 ccgtgcttca tattgaggtc aggcgcgtta aaaccgatag cgtcgatgct gtactgtttt
    11761 gccagccaga tggcgcgttg gttatgaaat tcttgcgaga taatggtgat atggttttca
    11821 ccaaaaacct ttttggcacg cactaccgaa tccagcgttg agaatccggc atagtcgcag
    11881 aagatcactt tcgccggtac gcctttagcg atcaaagcct tctgcatacc tgatgcttca
    11941 tcgtaatttt tacgtccgtt atcgccactt accagtagcc atttcacttt tccggcgtgg
    12001 tacaacgctg ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg cctggccccc
    12061 agcaataagc ccacgttacg cgccggaact gcgttgacat cgctccaggt cagctgcttg
    12121 ctggcgttga ccattactcg atcggcgata aaaatggtcg ctcctgccac aagtaacagt
    12181 aagcaggcaa tctttagagt ccggcgagaa aaacaaatac ggagaagaag gcggggctgc
    12241 atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag aaagaaaaga
    12301 taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg ttagccacag
    12361 ccgggtgtcg aactccagct ggtggtactg cggctccata tggcagcaca actggtaaaa
    12421 cgctttgtta tgctctttct ccttcaggtg cgccagttcg tgcaccacga tcatacgtaa
    12481 aaacggttcc ggcgcgttgc gaaacacagt agcgacgcgg atctctgctt ttgctttcag
    12541 cttgccgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt ttagcacatg
    12601 gatcttattg tcatacatca ctttattgat cggcggggca ttgcgcagaa actgattttt
    12661 tagatcctga gtatattgcc agagggcttt atcggtggcg tagtcgtgtg ttcccggata
    12721 gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agcgttcgca cctgggaaag
    12781 tagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc acgaaaaaga
    12841 gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta tcattcagga
    12901 gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg ttttccggcg
    12961 acggatgacg ttaacccgct acaggcgtgg gaagcggcgg atgaatattt gctgcaacag
    13021 ttggacgaca cagaaatccg cggcccggtg ttgatcctga atgatgcctt tggtgcgtta
    13081 agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat cagtgaactg
    13141 gcgacgcgcg agaatttacg cctcaacggg attgatgagt cgagcgtgaa gtttctcgac
    13201 agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc gaaaacactg
    13261 gcattgctgg aacaacaact gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt
    13321 attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt cgaaaaagtg
    13381 ctcggctcga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa ttgcacgttc
    13441 aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga aggtactgac
    13501 tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc
    13561 ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct cggttgcggt
    13621 aatggcgtta ttggtctgac gctgcttgat aaaaatccgc aggcgaaagt ggtgtttgtc
    13681 gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa catgccagag
    13741 gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga gcctttccgc
    13801 tttaatgctg tgctctgcaa cccgccgttt caccaacaac atgcgctgac cgataacgtc
    13861 gcctgggaga tgttccacca tgcccgccgc tgcctgaaaa tcaacggcga gctgtatatt
    13921 gttgccaacc gtcacctgga ttacttccat aaactgaaga agattttcgg caactgcacc
    13981 accatcgcca cgaataataa atttgtggtg ctgaaagcag tgaagctggg gcgtcgtcgg
    14041 taagattggg agttacctgc cggatgcggc gttaacgcct tatccggcct acgttcagca
    14101 cgttactccc aggccggata agacgcggca agcgtcgcat ccggcaatgt ctgcccagtt
    14161 ctggaatcgc gcttccataa aacaaaaaat ccctctcacc atcctctaca ttctcttgtt
    14221 tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatacgtt gccaatctgt
    14281 caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg cacctgataa
    14341 ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg gagttggtgg
    14401 ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta aaagcagtat
    14461 tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat gttgggggca
    14521 atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac atacgtgaag
    14581 ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag gggaaaaaat
    14641 gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgcggtgg taccttttct
    14701 ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag atcatctgct
    14761 gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa ccgcgtggga
    14821 agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc ctggcggtat
    14881 agccgtgatt cgtaccctta tggatcaata tggtttaacc cttgccgatc tgccggaaat
    14941 tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa cgctggaaca
    15001 cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg attaacgcga
    15061 cgtgccggat gcgttgcatc cggcgcttca aataacgatt taaatctcca gcgccagccg
    15121 tgttccctgg gcaattgccc gtcgtgcgtt cagctccata gccacatcgc agccgccaat
    15181 taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt ttggctcttg
    15241 ccctgcgcag atcaccacat tgtccacagc taatacctgc gtttcgccgt tgatcaccac
    15301 atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt tcacaccccg
    15361 cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct gtcccggttt
    15421 gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct gcattccctg
    15481 cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat tacagaacct
    15541 ggcgatattc tggctggttg attcgcccgg ctgacttaaa tacatcgccg tatcaaaacc
    15601 aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt cgcgcagtac
    15661 atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa cgcgcggcac
    15721 gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg tcacggtgtg
    15781 attgagtttc agcgtcacgc ccgtcacttc gatcatccgg tggtaatagc gcagcgtttc
    15841 gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc cgctctcgct
    15901 gtgggcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg caaacgccag
    15961 cccagcaggt cccgcaccga ccaccgccag attttttttc tgcacggcag gaaggattgg
    16021 cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt tgccaacgaa
    16081 gatctgatcg aggcaggcct gattgcagcc aatgcaggtg ttgatctcat cggctcgccc
    16141 cgattgcgct tttgacagca gttccgcatc agcaagaaac ggtcgtgcca tcgataccat
    16201 atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa tccggttggt
    16261 ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc taaatgcgcc
    16321 gcgcggcacg ggcgtggcaa tggtcggaat acgtgcttca tgccagccaa tgccggtgtt
    16381 gataatggtc gcgcccgccg cttcaatggc ctgcgccagc tctaccgttt cggcaaaagt
    16441 cccgccgtct tcgaccaggt cgagcatcga cagtcggtag ataataatga agtcgttgcc
    16501 gacgcgttcg cgcaccgcac gcactacttc tacggcaaat cgcatccggt tgcggtaatc
    16561 gccgccccac tggtcactac gctgattggt gtgcagcgtc agaaattcgt tgatcaaata
    16621 cccttcggac cccattacct ctacaccgtc gtatcctgcc tcccgtgcca gttgcgcgca
    16681 gtgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt gaggaacgaa
    16741 acggttgatg ggggcctgca atgcggaggg agcgaccaga tgcggttggt agctgtagcg
    16801 cccggtatgc aaaatttgca gggctatttt gccgccttcc tgatgtaccg cttcggtaat
    16861 ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt ccatgccaac
    16921 gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt gacgggcgcg
    16981 ttcggcataa aacgctgcca gccgctcggc accgtccggg tattcctcca gcccggtgtg
    17041 cattgagccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca gcggggcgaa
    17101 cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg tggtcggatg
    17161 agttctaatt tagccgtcgc cggagaaaag ggaaaagggg agtgcggggt ttgtgatggg
    17221 attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg ttttatccgg
    17281 cctgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc cagtggcgcg
    17341 gtgcagatgc cggatgcgac gcttgtcgcg tcttatccga cctacgggaa cgcacatgta
    17401 gggcggataa ggcgttcacg ccgcatccgc cagtggcgcg gtgcagttgc cggatgcaac
    17461 gcttgccgcg ttttatccgg ccaggaggat tgcacatgta gggcggataa ggcgtttacg
    17521 ccgcatccgc cagcagttgc aaattattgc ttacggaaaa aatcgttata caacatatac
    17581 aaatgcgcgg cactccagga gaaatttggt gcgccttgct gcgcgccagt cagcgggttg
    17641 taattctcct gaattgggcc atcggcggtt aatcctttgg cgtgccggaa gaacgtatcc
    17701 gccagcttca gggcatcatc acgataaccg taacgctcca tccctttcag agcaaaccag
    17761 aactgatcca cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt
    17821 aacgccgccg ttcccagcgg gacaaaggtg ttgaactctt taggatcgag catcaccttc
    17881 accaccgcgt cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc
    17941 ggccctttac cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct
    18001 tcaatacgta cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag
    18061 tccgcgagct gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg
    18121 gttgccatct ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac
    18181 tcctgcaata gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc
    18241 accgtccagt cgctacgttt gccaccatta gcgacatatt tatccaactg ttctttgtcg
    18301 ataaaaccaa agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc
    18361 ggaatttcca gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca
    18421 gactgcgtct cttctttgtt gcctttcttc accgcaaaca gcatctcgcc gctctcagtg
    18481 ttgtgggctt tgtcgcgggt cgcgccatat tccggcacgc cgttgccgtt gtgatcgcgg
    18541 ttacgtaacc accagtcgtg ataggcgacc agtttcgggt acatctctgc caaccaggct
    18601 ttatcctggg tgacgttgta cacttctatc accgaccagg cggcaaggct gggtttggta
    18661 ttacgttcgt tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc
    18721 aggtcgggga caaagcccac atcctgcgga cgcacgcggt cgccaggctg gatctgccag
    18781 gagaagaccg cgcggatatt ctctttggcg atgtccggat tgaaatgcgc catcgcaaac
    18841 gcctgcttct aggtatccca cggccaggtc tgattgccgg agaaccagcg cccggtcacc
    18901 gacggtgtaa cggtgttaaa tttcaccgca ccgcccggtg agcgccagtt accgttgagc
    18961 gtttcgatcg ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac
    19021 cctttcttca ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc
    19081 gccagaatat cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg
    19141 gaataggtgg tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta
    19201 ccgttgattt cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag
    19261 gtcagcagat cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg
    19321 gcgctgattt tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt
    19381 ttcccttctt tcgcttccag tttttccagc agttcgccgt cccacaccag atccagcggt
    19441 ttattgctgg tgattttggt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc
    19501 atttcgactt gcacatcttt tgctgtcagt ttttgcacca gcgcaccggg aatactgtat
    19561 gcctccagcg taaagtcgac tttcttgccg tcctgccaga cggtcaggcg gtcgaaattg
    19621 ctggccataa agttgatgta ctcttccgtc agcagcgcaa cgcccggaaa gccgcccatg
    19681 gtgttagggc cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta
    19741 aaacgctggt gatcgtcgta atcgtaatcc ttcatgtact gcggcgcgcc agtacggtta
    19801 atcacgtttt tataattgtc ggcgttagcg gcatgggcgg aaaaacttat cagcagagcg
    19861 caggttactg gcgttaaaat agtttttatt ttcatgaaat tacagtcctt attaccagta
    19921 gaaatattgc atcaggaaga tttggtcgct gggatcgtga gaggcatttt tcatgtagaa
    19981 gcggctatca aaagccgtgg cgaaacgtcc gccgccgtat tcgtaccaga taccaaactg
    20041 catgaaagag gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc
    20101 ggtggagaga taaacgccct ttaacgcttc atacatggcg ctcaccatca tcccactttc
    20161 acttcccggt acgtccagcc cgtcggcgcg tccggtatgg tgccaggcgc gagcggcaaa
    20221 gttaggagtt agcaggcccg tgagatagag ctgtcctgtg ccgtcggtga tttccagacc
    20281 gttcatccag gtgatgcctt cggcaaggtc atactggatg taaccgttca tcattgccgg
    20341 ataggtgtat ttgtcgtcat agcgatcgta tttgccaaaa tgtagccagg ctttgctttc
    20401 atcatgatgt ccggcaggag tcgcggtgac gctataacga aagttgccgc ttaagttctg
    20461 taccttcagc gcagccataa tgtcgcgggt attgggaatg acgtagccca gatcgggggt
    20521 gaaatcgccc caccattgca ggtcatccag cgaggaatcc taacgcagac tgagcatgat
    20581 ttcggtgcca tcattggtac gataaccgcc atagaaacgg ttaattccgc cctcgaaacc
    20641 accccagcta tggtctggca c
//
LOCUS       DAJTOR010000063        20505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTOR010000063 DAJTOR010000000
VERSION     DAJTOR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.063"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..4317)
                     /gene="mukB"
                     /locus_tag="OO249_002023"
     CDS             complement(<1..4317)
                     /gene="mukB"
                     /locus_tag="OO249_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5636329.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVCADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NIS"
     gene            complement(4317..5021)
                     /gene="mukE"
                     /locus_tag="OO249_002024"
     CDS             complement(4317..5021)
                     /gene="mukE"
                     /locus_tag="OO249_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5636330.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5002..6324)
                     /gene="mukF"
                     /locus_tag="OO249_002025"
     CDS             complement(5002..6324)
                     /gene="mukF"
                     /locus_tag="OO249_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5636331.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLQVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6321..7106)
                     /gene="cmoM"
                     /locus_tag="OO249_002026"
     CDS             complement(6321..7106)
                     /gene="cmoM"
                     /locus_tag="OO249_002026"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5636332.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7242..8021
                     /gene="elyC"
                     /locus_tag="OO249_002027"
     CDS             7242..8021
                     /gene="elyC"
                     /locus_tag="OO249_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5636333.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7998..8891)
                     /locus_tag="OO249_002028"
     CDS             complement(7998..8891)
                     /locus_tag="OO249_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5636334.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQEHFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9045..9791)
                     /gene="kdsB"
                     /locus_tag="OO249_002029"
     CDS             complement(9045..9791)
                     /gene="kdsB"
                     /locus_tag="OO249_002029"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5636335.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9788..9970)
                     /gene="ycaR"
                     /locus_tag="OO249_002030"
     CDS             complement(9788..9970)
                     /gene="ycaR"
                     /locus_tag="OO249_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5636336.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10022..11254)
                     /locus_tag="OO249_002031"
     CDS             complement(10022..11254)
                     /locus_tag="OO249_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5636337.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLALEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11291..12277)
                     /gene="lpxK"
                     /locus_tag="OO249_002032"
     CDS             complement(11291..12277)
                     /gene="lpxK"
                     /locus_tag="OO249_002032"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5636338.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12274..14022)
                     /gene="msbA"
                     /locus_tag="OO249_002033"
     CDS             complement(12274..14022)
                     /gene="msbA"
                     /locus_tag="OO249_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5636339.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14059..16331)
                     /locus_tag="OO249_002034"
                     /pseudo
     CDS             complement(14059..16331)
                     /locus_tag="OO249_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16538..16822)
                     /gene="ihfB"
                     /locus_tag="OO249_002035"
     CDS             complement(16538..16822)
                     /gene="ihfB"
                     /locus_tag="OO249_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5636340.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16982..18655)
                     /gene="rpsA"
                     /locus_tag="OO249_002036"
     CDS             complement(16982..18655)
                     /gene="rpsA"
                     /locus_tag="OO249_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5636341.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18766..19449)
                     /gene="cmk"
                     /locus_tag="OO249_002037"
     CDS             complement(18766..19449)
                     /gene="cmk"
                     /locus_tag="OO249_002037"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5636342.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VATFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19622..20385)
                     /gene="ycaL"
                     /locus_tag="OO249_002038"
                     /pseudo
     CDS             complement(19622..20385)
                     /gene="ycaL"
                     /locus_tag="OO249_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
BASE COUNT         4941 a         5677 c         4976 g         4911 t
ORIGIN      
        1 gctgatattt tccggcgctg cgatgatgag ctgcatttgc aaacgctcac acaattcaaa
       61 cagcgtggcg atagaacgag catccagtcg cgctgcttca tcgaggaaca gcagacggca
      121 aggagagata tctttaccgc gcaggcggcg agattcatct tcccagcttt gtaccaccat
      181 caccagaatc gacataccgg taccaatcgc ctcaccggtc gacaatgcac cagactctgc
      241 gcgcaaccaa ccatcggaac cacggttaac ctcaacttcc atttccagat agttgcggta
      301 atccagcagt tcttcgccaa tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt
      361 aagacgctga tacaatttcg ccaacgcttc cgagaaggtc agacggttgc tgttaaacag
      421 atcctgatgc tgctcgtgct gttcagagag cacatccagc agcatggcgt gcgtttcacg
      481 cacgttcacg ttgaggcgca cgctgttcac ctgaccaaac gatacgttct gcaacccctg
      541 gttgagcata cggatacggt tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac
      601 gctgcgggaa ctgatcacca gtttctgttc acgggaggtt aattcttcgg tcagacggct
      661 aagttcgatc tccatctgtt cgatagcttc caccggatca tcggtacgaa taatatcctg
      721 acgaatacgt tcacgcagat gctgataagc cgccacgaag aactgaattt tacgctccgg
      781 acgtttcgga tcttccgaca tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc
      841 cagacgcagc gcacccaatg ccttatccga catagagcgc aaatcatcag cggagagata
      901 agccagctca cgacggtgta agcggcgctc aacgccgtta tctttcacca tgcgcatcac
      961 cgcacaccag cccgctttgg cggtcactac ctgctcgcgc atctcaaagt aatcccgctc
     1021 cagcttgcgc agtttgcggg tcaggttgtc catctccgct tcgcagaagg taagcgcttt
     1081 ttccagttga ttgcggcgtg aacggttatt gctcagttgc gcatgcagct cgtcacggcg
     1141 aatacgcgcc cgctcttctg ccccgctatc agcacacacg ccgatatcct gcaattcacg
     1201 ttgcagatcg ttgagtagct cttttttggt gtcgtaagaa cttttcagcg aagccagcac
     1261 ctggttgtac tgactcaact gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg
     1321 ctccgcttcc gcctgttcca gacgttcacg cagtttttcg ttgagatcgc tgttaccgct
     1381 aagcatttct gccgagtcag agtagctaaa gtgcgcacga cgctgcacca cttccgtcag
     1441 ggcaaacgcc tgctgacggg catcgcgctg catctgttga gagtacgcgt aatcttcttt
     1501 taactgttcg aactgttccg ggtcgctctg caataccgaa acgatcggtt ccagtttcgc
     1561 cagttgattg ccaaactgct gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc
     1621 gcggatttca tcgacgcgat ccgccaggct atcatcagcc aacaggttga gacgcggcag
     1681 aatgcggttc agcgccgtaa cgccctcttt cgcctgctca aactgaatac gctgctgctg
     1741 gttatcattt tcatgattac ttaacgcccg ctccagttcg acgcgacggc tgttcagttg
     1801 acggatttct gcttccgggt cagactcaaa cacaaccgcc agatgactgc cgataaagcg
     1861 gctgaacgcc tgatgcagac gctgagtttt ctgtacatca aaggagagcg tggcgaagcg
     1921 ttcggaaagc acttcacgct cggcatggag gctttcaata cggctttcac gcgcagcacg
     1981 accaaacagc ggcacttccg ggaaacgtga ataacgccac tgacgatcgg cgattttcac
     2041 cactaccgct ttttccagct catcaacgct gaacacgctg tcatcgaatg actgcggatc
     2101 tccttcgatc agatagagat cttccgggca atcggtcaag ccatctaggt gttcagttac
     2161 ctgtgacaga tctggcacca cgatggcgtg gcgtgacggg ccatacagcg ctgagaagta
     2221 cggcgcatct tccaggctaa cgtcgtcata aatttctgac agcagcacac caccaaaacg
     2281 ctccgccagc gcgttcagac gctgatcttc agagccgcca ggctggctta aacgttcgat
     2341 ctcttcatcg acggcgtttt tgcgcgcgcc cacttcatcg cgttcaacaa tcgcctctcg
     2401 ctcacgctcc agcaactgtt gcagatattc ggtgacgtcc tggctggagg taaactcttc
     2461 gccgcactgt tcgctcaact ggttgagact gttttgcgct gccagccaaa ccggcgcacg
     2521 ctgcatcaaa ctctgaatgc gagactgcag ctgttcctgc tcctggcgca gtgccatgcg
     2581 ctcttcacgg gcgttagaca cgctatcgga aagagaggca atgcgtgctt ccagttcctg
     2641 atgcagggct tccagttcgt cgatatcaaa attcttgccc tgacgtttgc agaaatctgc
     2701 cagcagacgc tcagcttctt gctgctcgcg tagacgctgt tccagttcgc ttaatcgcat
     2761 ccgcagcggc tgaacctgct ctgccaggtg acgctgatcg accccttcgc gcaatagttc
     2821 gcgagcgaca tcccacgcct cgttacgcgc cagtgggccg ttgattgcca ccaccagctg
     2881 ataagcctgc tcaaactggc tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga
     2941 gagcattttt tcagtcgctt ccagctcttt cgcctggaag gtttccagcc attcggcggc
     3001 gctgtcggcg gttaagtccg gcagatggca cagttctttg gcacgattaa gcgcagcaat
     3061 cgcctggtta tactggatcg cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc
     3121 aagctggctt ttcagctcgt ccacttccag ttcggcagct tccgcgcgag cctcgttctc
     3181 ttcctggcgt tcgatggctt ctgccaccac ttcgttttgc tcttccagac ggatctgcag
     3241 ctcatcgaga tccgcttcgt agcgttcgat cttctcctgc tgacgcagtg cggtttgcac
     3301 caggttcagg tgatcgctgg ccgcctgata atccgcttcc agatcgcctt cggcaccgtt
     3361 gtgctctgcc agctcacgcg ccatatcgac gtgtttgtac tgctcagccg ccagttgctg
     3421 acgcgaagta tgtaactcgc gacgaaactc cagggctttg tcgagatgga cacggcgctc
     3481 gttggcgtga cgcatgtagt ccgccgccac gtagttggta gcttcgctga tcagatgctt
     3541 aaacaggtca cggtccgact gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg
     3601 cagcgccgct tccatgtcct ggaacgcttt acgcacgccg ctgttttccg gcaacagata
     3661 gtcgcgcaga gaacgggtaa tggcactgga gatcccgcca tacagcgaag cttcgatcag
     3721 acgatagaat ttgctacggt cagatgcgga gcgcagacga cgcgcgatga tgcccagatc
     3781 gaacatcagc gagtggtaat cggtaatgga gttgaactgt ttaaactgca cgccttccat
     3841 cgcctcgagc ttgtctttca gctcgttaag cggcagcacg cgcgcctggc gttcgttcag
     3901 ggtttcggtc accagctgtg tcggctgcac cgacatcggc agtccctgaa tggcaaatgg
     3961 tttgatatcc actttacgat cgcgtccggc aacctgttgc agacgcacac cgaccaccac
     4021 gcgctggtgg cgcgagttaa tggtgtcgag catcgaataa cagacacccg ctttcagctt
     4081 accgtgcaga cctttatcgc gcgaaccgct ggtggccccg gcttccgtag tgttacggaa
     4141 atgcagcagg gtcaggtcgg ggatcagcgc cgtaacgaac gccgccatgg tggtggattt
     4201 acccgccccg ttaccgccag aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc
     4261 aaaaaagccg ttccagttaa tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat
     4321 tcttcctctc cgctatctgg ctgattctct tcggtttcat cgttgagttg cagatgattt
     4381 tcaatcggca ttgcttcgcc atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg
     4441 ccagcacgca catcggctcc gaagcggaac accgattcgg taatgcgaaa cttgctgctg
     4501 tcgtggccca taaaccacac catgcctaaa cgacgcagac ggttgagcga agaacgtact
     4561 ttctcctgca acttctgacg gtcaacgtct gaaccggttg aacggttgtt caccagtttc
     4621 agcagttttg cttcatcggc cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa
     4681 atcccctcat tcgccagccg ttccgggctg agatagagat aacagaggat tttcccgacc
     4741 atcatatcca gttccgacaa gacggaacgc gggataagag tggtggaacg tgggcgtaaa
     4801 tagaagaacc cttccggtgc gcgaataagc tcaacgttat aacgcgcgta aaactcttcc
     4861 agatattcct gaaaatccat caggaatgca tgattatcca gttcgtcgag gccaatatgg
     4921 cggcctgaac gtaaggcgct gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc
     4981 agcttaaccg gcatcacttg ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc
     5041 gtaatcatta atcggctgcc atttcgctgg cagtccggtg aaatctgctt gcgctacgcc
     5101 aagacgtacc gcctgatcaa taacaatacg cgcaacgtca aagtgacgtg cacgcggata
     5161 ctgtgacaga tattcgcgta ccaccagacc aagatccagc ggcacttgtc tggttttgta
     5221 cacggcaagt tgttcttcga tgatcgccgc cagctgttcg cggatctcgt taaactcttc
     5281 gtattccaga tcctcaggaa gttccccagt cacttcttca tcgcgcagtg ccatctcttc
     5341 gtcacgcata tccagcagac gatcggcatt ggcataagtt agcgcccacg gctcatcaaa
     5401 ataggtttgt accgactgac gtaaccgctg agcaaagacg cggtttttat ccatatcgat
     5461 cgcggtacga ataaatttgt gtacgtggcg gtcgtagcca atccacaagt cgatggattg
     5521 ctggccccaa ctgataatac gatcgagttt gctctgcaga tcgaacacca gacgatcgac
     5581 gaaatgcaga tcgtcatggg tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa
     5641 tttgtcgcct gccgcttcca gcgtatcctg caattcacgc agcgttccgg aagtttccga
     5701 aagcaacaat tcacagctgg aaatcgccgc ccgccagtct ttgttcagca actgggcgat
     5761 atcgtccttc acctgctgct gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc
     5821 gaaaatttct gctaccgaat atttcagtgg cgcatagaca ttacggtgcc agtgaaattc
     5881 atcaccgccc tcttcggcgg catctgctgc gcgtttgagc tcacccgcca caatcgacaa
     5941 ctgcatagaa agacgcagcg tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc
     6001 gatgccgagc ggcgtcagac ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg
     6061 gttcagcaga cgttgacgca ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt
     6121 ttcgctggtt tgctcaaacg catcactcac atggcggaat gcatccacca gctcgccttc
     6181 actcatctca ccatccagac gctcgccgtt cagcgtggca accgccagca gaaaagagag
     6241 tcggtctacc tgcagcgaga tggagaagtc attttttctg gcccaggcaa ccagttcggg
     6301 gactgtctgg gaaaattcac tcatacttta tccttgctct gcggtttgcg cgcggtgaca
     6361 tgaatataac gccccagggt aatatacggt tcctgacggc aataacgcgt ttctaattca
     6421 agtaatgctt catagcagtc gcgctgctgg tgcttctcgc gcagataatc atgaaacacg
     6481 cgaacgcctg tcttacccat aatttgccaa ccagcttctt ccagccacag ataaacctgc
     6541 gccgggtcgc gtggataatc tggcgaaagc gtccgttttt tctttttcgg cattcccgcc
     6601 tgcacgtaat caaaattccc ggcgaccatg ttatgcatca acaaaccatg cgcattgtag
     6661 aacattaacg acaacacgcc gcctggacgc aacactgacc acagggtctg caatacactg
     6721 cggggatcgg ccacccactc gagcaccgca tggaacaata tcagatcaac gggcgtttcc
     6781 aaatgcgaag caacatcctg agcggcgcaa tgtataaatt gcatgttgtc gctcacacct
     6841 tttgcttctg ccgcctgttt tgcgcggtcg atcatctgcg cagaaagatc gcataaaatg
     6901 acctgatgcc cacgctcggc cattttgatt gcggtctgcc cttctccacc gccagcatcc
     6961 agcacacgca gtttttgcgg gcccatttcc gccagcacgc gatcgagatc ctgccacaga
     7021 atagcctgtc gaagctgccc tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca
     7081 atatcatcaa aattgcgatc ctgcattgct ttctccaccg gttggctaag acgttttctg
     7141 ataaagacgc tattttgtca cagccacggc gagaatgaac ccgtctggtg taatatcgct
     7201 ggcgatccct tgctatatgg ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa
     7261 aagtgattgg caatatgttg ctgccacttc cgctgatgtt gttaattatt ggcgctggcc
     7321 tggcgctgct gtggtttagc cgttttcaaa aaaccgcgaa gatttttatt agcattgggt
     7381 ggctggcact cttgttattg agtttgcaac cggtagccga ccgcctgctg cgtcccatcg
     7441 aaagcaccta tccgacatgg aataattcgc aaaaagtgga ctatatcgtg gtgctcggag
     7501 gtggttatac ctggaacccg cagtgggcac cgagctctaa tttaatcaat aacagtctgc
     7561 cacgcctgaa tgaaggtatt cgcttatggc gcgaaaatcc gggatcaaaa ctgatcttca
     7621 cgggaggcgt agcaaaaacc aatacggtga gtacagcgga agtaggtgcc agagttgcgc
     7681 aatcgctggg cgtgccgcgc gagcaaatta tcaccctgga tttgccaaaa gataccgaag
     7741 aagaagctgc ggcagtgaaa caggcgattg gtgacgcccc tttcctgctg gtgacctccg
     7801 catcacatct gccgcgggcg atgatctttt ttcagcagga agggttaaat cctctaccag
     7861 ccccggcgaa tcagttggcc atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt
     7921 ctccggtctg gctgatgcat agcgatcgtg ttggctatga aacgcttggc cgcatctggc
     7981 agtggctgaa aggtccgtca ggcgagccac ggcaagagtg attttgatgc cagatcgaag
     8041 ttgcgccgac taaatcgtcc ggtattaacc agttgcgcaa cttcatccca taacacatac
     8101 aaccaacgcc gccagatgaa cgactccgcc actggcgcgc gttgcaggta actccagagc
     8161 aaatcttccg ccagagaatt atccattagt cggaacagtt cgtattctcg cggtgcccaa
     8221 agcattagtc ccggcccgac catcgccagt aactgatcgc tgcgcgaatc tttcagcatg
     8281 ctgcgtaaac agaagttacc gtgaatcaac acacaattgt cgttaaagcc ttcaaataat
     8341 gccgggagac attcgcgagt gcgaaacagg atccgcttat cctgcatcgt cagaccggtg
     8401 ttattgaact gattgagcgt ggtccatagc acttcaacat gttgccggta ccatgagggc
     8461 cagaaatgtt cctgagtatt gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc
     8521 caggccagta aggcttcaac gatttggtct ttgagctgtt cccagcgttc tggtgttcgg
     8581 gctggtgcct ccaccgaaac accacgcatc cgctccagta gcaggacatc agggccgggg
     8641 tgttcttcat gcgtcatcac accataaata gtcggcatac ggacagtccc acttcttgcc
     8701 agcatggtgg ttttccatgc cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc
     8761 attaacggca ttgggtttcc ctggctgtca tacaaagccc acaacgccgt atccgctttt
     8821 tcattgacgc actcaatacg actgagtttt tcgcccagta aatggcttaa ttcggcacgc
     8881 agctgttcca ttcagattac cctcataaaa gctactgctt tcataatgag cgccaatata
     8941 aaagatgtca cctgaatgag atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg
     9001 tacgacactc ctcccaaaat tggctgaagt gtcgtgaagt gaaattagcg catttcagcg
     9061 cgaacgcgct caagatcttc aggggtatcc acacctgtgc caggaacttc ctgagcaaca
     9121 gcaacatgga ttttttcacc gtaccacaga acacgaagct gctctaacat ttcgatgtgt
     9181 tctaacggac ttgcctgcca gttgacgtaa cgacggataa agcctgcacg gtagccataa
     9241 atgccaagat gacgcaggaa gttatcgcca acggtttcaa ggccttctgc aaaacgatca
     9301 cgatcccaag gaatggtggc gcgtgagaag tacagtgcat acccttcagc gtcgagaacc
     9361 actttcaccg cattcgggtt aaacgcttct tccgcattgt ggattggcac cgccagagtc
     9421 gccataccca cctgacgctg agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg
     9481 atcatcggtt catcaccctg cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt
     9541 tcgacaactt ccgccagacg ttctgttcct gactgatgat cggcgcgcgt catacatact
     9601 tcaccgccag cggcttcaac ggcgcgggca acatcctcat gatcggttgc cacgatgatg
     9661 cgctcggcac ctgattcacg cgcgcgttca agaacatgaa caatcatggg tttgccgtta
     9721 atatcaacca atggtttacc gggcagacgc gtggacgcgt agcgcgcggg aataatgacc
     9781 acaaaactca tgatttactc tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca
     9841 ctggaatgcc atcgcggagc gggaaagcca ggttatccag tttgcaaata agctcttgtt
     9901 tttcctggtt ataccaaagt tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac
     9961 gatgatccat agttcctcca gatggatcgg gttatgaatg cataaatctt atcataatca
    10021 tttatgcgac ggggtctatt tcccagccag tgcggcaatc agtaaagaga ccttgcgggc
    10081 agcgtcctaa tgtcacccgc gttgcctgct gccagctcgc gaaatcagta atcgcctgac
    10141 gtaacccttt ttgcagcatt gtcgttggtt taatgccttc ctgtaaccac agagagataa
    10201 cttcaaggat gcctgtctgg cgatgcattt tggcatccat tcgcccaact aattgcccac
    10261 gatgtaataa tggcagaaca aaatagccat actggcgttt cggcgctggg gtatagcact
    10321 ccagccggta gctaaaatca aaaagctgct ctgcgcgttt gcgatcccag acaacaggat
    10381 caaaaggcga aagtaccgcg ctgtgcgttg cagtgagctt tccggccagc gcacgctcga
    10441 gtagcggcag caaatcagca tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa
    10501 tgatttgctg ctgttcagcc ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc
    10561 gatagtaatc tgccagccac tgttcgcgga atattcccag gctacgcgca ctgttatcca
    10621 gcatgatgat ttctgcttct gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca
    10681 tgacacggtg ggttaaatca taaacgcgct ggaagttgcg ccgttcaatc accatcacct
    10741 ttccggcagt aaataaacct tccagatgcc gtttatgcgg tttccattcc caccagccgc
    10801 ttgcaccttt acgaggatgc tcaaaatcgg ctgaacgtac cggcccctta tcatgaatat
    10861 gctgaattaa ctgtgcaatt tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc
    10921 agcccatttt ttcaagtgcc agcatgcggt ggcgaataag acgaaagtcg ctacgcggca
    10981 taaagcaggc ttcatgcgcc cagtattcca ttaattcgcc acgcgccaga gactcatcca
    11041 gccactgggc aggataattt cccagacgac tgaaaagcac cagatatgga ctacgggcaa
    11101 caatattgat ggtatcgatt tgcagcaagg acatgcggga gatcgttgcc ggaatatcct
    11161 ccaacgacgc tcgacggcgg ggtttgttta acaggccttg tgcggcaagg tgaagattac
    11221 gcgcatcagc aagggagagg tgcggcagcg acattcatga ctccatcaat cgaacgctgc
    11281 cgcggcgtaa ctagttgcca gaagccagcg aggttagttg cgtaagcagt ttcgctggtt
    11341 catcacctga aagctgtgcg tctacaggca aataccacca attttcttct gcaaaggccc
    11401 ggcatttcac cgcatctttt tcagtcatta ccagcgtttg cccggcgctt accaacgcac
    11461 tgacatccgc atggttcaaa gactgatgat cggccagcgg tacacatttt tccggttgta
    11521 cgccacacat cttcagcgtg gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca
    11581 ctacatgttc aagctgagca acgtcacaac gcgtaccggt acgtaaattc accgcctgac
    11641 ccggcagcag atgcatggga atttcaccgc tgcgagggac accaccgttg acgattaccg
    11701 catcaaccga ctttaagcgc cccgctcgct cacgcattgg ccccgccggc aaccaccagc
    11761 cattgccaaa gcgacgcaca ccatcaataa cgacaatttc cacatcacgc gccagacggt
    11821 aatgctgtaa accgtcgtcg gttacgatga tctgcacatc agggtgttgc gccagaatgg
    11881 cttttaccgc atcagaacga acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa
    11941 tcaacacagg ttcatcaccc gcctgtgctg ttgtggtatc tgccgacaat aacagcggat
    12001 aagattcagc cttactacca tatccccgcg atacgacccc cacgcgaata ccgcgctgtt
    12061 gcaactgttc caccagccag acaacgaccg gggtttttcc gttgccgcct gcggtgagat
    12121 taccaaccac gacaaccggt acgggggcac gccaggcgcg cttcagtttt agtttatagc
    12181 aaagacggat cgcgccactc accaggccat acaaccagga gagtggcagc aatagccgcc
    12241 acaaagggga ttcaccagac cagatttttt cgatcattgg ccaaactgca ttttgtgaag
    12301 ttgcgcgtaa acgccgcggt gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac
    12361 accatcctcg acgaccacga tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat
    12421 caccagagag gtacggtttt tctgcaactc atccagtgcc gcctgaatcg cacgttcgga
    12481 ttcggtatcc agagccgagg tagcttcatc cagaatcaga atcgggctat cacgcagcaa
    12541 ggctcgagcg atagcaatac gctgacgctg accgccagag agcagcacgc cgttttcacc
    12601 aatcactgta tcgagaccgt tatccatctt attgatgaag tccatggcgt aggccatacg
    12661 cgccgcttct tcaatttgct cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt
    12721 agcaaccgta tcgttaaaca gatggacatt ctgcgacacc agagcaacct ggttacgtaa
    12781 cgacgccagg gtatactcgc gcagatcgtg accatccatc aggatttcgc cttcatcaat
    12841 atcgtaaaaa cgagtgatca ggctggcgat ggttgattta cctgaaccag agcgtccaac
    12901 cagagcaacc gtcttccctg ccggaatttt caggttgatg ttacgcaatg caggtacgtc
    12961 acgtcccgga taagtaaagg tgacattgcg gaattccacg tcgccagtcg cacgctcgat
    13021 cacgcgctta ccttcgtctt tctcctgctc actgtccaga atggtaaaca gcgtctgaca
    13081 agccgccata ccgcgctgga actgggcgtt aacgttggtc agtgatttca gcggacgcat
    13141 cagtgcaatc attgaagaga aaacaacggt aatcgtacca gcagtcaggc tatccatgac
    13201 acttgggaag ctcgccgcat acagaacaaa cgccagcgcc aaagaggcga tcagctgaat
    13261 gatcggatca gagatggaag aggctgaaac cattttcatc ccctgaagac gcattcggtt
    13321 gctgacttta tcaaagcgtt tcgtttccac ttcctgacca ccgaaaatca atacttcttt
    13381 gtggcccttc agcatttgtt cggcgctggt ggtcacctgc cccatggtgt tctgcatgtt
    13441 tttactgatg ttgcgaaaac gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc
    13501 cagcacaatc aaaatgatcg acagttgcca actgtaatag aacatcatga tgaacaggcc
    13561 gatgattgac gcaccttcac gcacaaccgt tatcagtgcg ccggaagaag aagaagcaac
    13621 ctgttcggaa tcgtaggtaa tacgtgacaa cagcgtcccc gttgactgtt tgtcaaaaaa
    13681 tgaaaccggc attcccatca tgtgaccaaa caggcggcga cgcatggtca ttaccacctt
    13741 tcctgatacc caggagatac agtagctgga gacatagctg gtgataccac gtaaaatcat
    13801 cagcccgatc accaccagcg gcatccacac cagcacggag cgatctgttt taccaaagcc
    13861 atcatcaaga agtggcttaa ggagcgataa catgaaggta tcgctggctg cgttgaggat
    13921 taacgctacg cccgccacga tcagacccgc tttaaaaggc gcaatggttg gccacagtcg
    13981 gcggaacgtc tgccacgtag agagatcttt gtcgttatgc attcaaaaaa ccagcatttg
    14041 ttgaaatagc cgcatattct acccgttatc cactggcacg ccaaaccact gatgatacca
    14101 acgaggtaaa atttgctctc tgaggctgct aatccgccag ccttgcgctg aaaaattgac
    14161 cgtcacttga ccctgatgtg gagtatcaag ccattgatat ccttgctgtt gatagcgatg
    14221 cttaacttta ttagagggca atcgccatgc gttatagcgt tatagcgcga tgccgatgcg
    14281 agtgccactt ttccattcac tcgctgaatt aatggcaacg atgatgaggt attactgcca
    14341 tggtgaggta cctgaagcaa tgttgtctgc acttgctgcc agtaacggct tagcatcttt
    14401 tgttcagcgg ggacttcaat atcaccggtt agaagaatgc tattcgtccc gtcataaacc
    14461 ttaaccacac aggaatggtt atttccttta tcgttgctag cttgtaaagg ccagtgcacg
    14521 ctgaaacgca atccttgcca ttgccacgct tcgccacgca cacagggctg atgatgttcc
    14581 cagtttaacg gacttctgat ccataacatc ggccatgtgt gcaatattga gtccagccct
    14641 ccccggtgat ccagatgttc atgactcaga ataacgcctt ccggttcaag attatgccag
    14701 tggagccagg ggataatcag ttgttgccca ctatcccctt cgggccaggc cagtcctgtg
    14761 tcatagagaa tcgctttgcc gtttctggct atcaccattg ccagcccttg cccgacatca
    14821 agcatgtaca cctgccactc gtcaggtcga ggtttttgcc acagcggcca gcacatcagc
    14881 aagcctacag ccacacacat tgctggcaac gttcgccagg cgtttaatcg ccataccacc
    14941 agtaagaacc atggggaaaa tgatagccat tgccaacgtt cagcaatgtt gatccaccct
    15001 tctggcaaac tctttaaccc ccagaaaagt aaagccaaag accggtcggc aagaaaccat
    15061 aacccttgct ccaggattaa cggcccgctt aaatgcacaa ccatcgcggc gaggatcaac
    15121 ggaaccgtga taaatgtcac caagggaatt gctaatagat ttgcaataaa cgaggtcaga
    15181 ctaatgccat gaaatatgac gatttgcaca ggcataagca ggagtgtgat tcccagttgc
    15241 aaatgaatga gggaaacaac tgcacgcaat accggcggca gttgccactc aggacaggga
    15301 aaccactggt accaaaaaat cagtgccgcg accgcagcgg cagagagcca taaactttgc
    15361 gagagaatgg caacaggatc catcagcaaa attgccgcca gacaacatat ccatacatcc
    15421 cagccactcc actgtcgccc acttaactta agcattcccc acgtagcaag cgccaccacg
    15481 gtacgcaatg caggaggttg cattcccgtc aaccaggcat aaaaagcagc acagcagatt
    15541 ccaccaatta atggcatttg ccagtggatc cagcgcccag gcagaaaaac ttgcccaccg
    15601 cgaatgagtc cggcagccag caacgccgca aaagcgatgt gcaatcccga tatcgccatt
    15661 aaatgcgccg ttccagtatc acgcatgata tttttgattt ctttggggac ggataaccgt
    15721 tcccccatac ctaaaccaag aataaccgca ttccacatat agggttgcag cgttgtttgt
    15781 agtgacgcca gatactgtgc acgcaggcta caattcggtt caatgacact tgcctgcaga
    15841 aagcggccga tgagcggctg atgctgggca atggcataac gctggctatc aaagccgcca
    15901 tcattaagtt ggccatgaac tgcacgaact ttgagtttca ttgaccattg ttgtccagca
    15961 caaaccgctt gcggaagata ttcgccatac agcacaagac ctggcgcagg gaatatacgt
    16021 cgaccttgta gatgagtaat ttgaccgtaa tgagtggtca tgccgtcagt tgcagtgatc
    16081 tcaacaattg catcctgcgt cgcgccagtt aaggtttctc ctgcccacaa aatttgcttt
    16141 gctgataata tgccccaaac gaaaaacagc aacgtcagcg cgatataacg gacggtttta
    16201 acagggataa atgccagtac gcaggcgaag agagtcagaa acgcaagggt taatgtccca
    16261 ggcaattggg gcaaaatcag caacggaaaa attccgcaaa ttatgcatac gccgactgtc
    16321 gttattttca tgcacccctc cctgtttatg gaaagtgtgc aactttgttg agcggttgtc
    16381 tggtgggaat tttgcgttcc ccgaggcaca ttcagaagaa tttaacgaaa gggtgaaaac
    16441 tgctcaaaac tctgcgaggc agcttccagt tcctgattag agaaatgaaa aaagcacccg
    16501 acaggtgctt ttctcgcgtt caagtttgag taaaaactta accgtaaata ttggcgcgat
    16561 cgcgcagttc tttaccaggt ttaaagtgag gaacgtattt tccttccagt tctactttat
    16621 cgccagtctt cggattacgt ccggtacgtg gtgcgcggta gtgcaaagag aaactgccga
    16681 aaccgcggat ttcaatacgc tcgccctgcg caagagtcga ggccatatgc tccagcatct
    16741 cttttactgc atcttcaacc gtcttggcgg gaatgtgcga ttgctgggtg gcaagtcttt
    16801 ctatcaattc tgacttggtc atgattcctc cggttcctta aaggcaaatt aagcggctgc
    16861 tgttgctgca ttgattaagg gcggccgtag ccgcccttag tgcttgatta caggacgaaa
    16921 cctgcaatct gtcaagtaaa ctcaacaaac ttcggaataa aaatcccgaa gagtcagaga
    16981 attactcgcc tttagctgct ttgaaagctt cagccattgc gttgttggag aagtttgcat
    17041 cttcctgttt gttaacagtt gcgattgcat ctttctcgtc agcttcgtct ttcgcacgaa
    17101 cagacaggct gattgcgcgg tttttacgat caacgccggt gaatttagct tcaacttcgt
    17161 cgccaacgct cagaaccagg gtagcgtctt caacgcggtc acgggatgct tcagaagcac
    17221 gcaggtaacc ttcaacgccg tcagccagtt ctacggttgc gcctttagcg tcaactgcag
    17281 ttactttacc ggttacgata gcgcctttct tgttcagagc aacccagttg ttgaacggat
    17341 cttctgcgag ctgtttaacg cccaaggaga tacgttcacg ttctgcgtca acctgcagaa
    17401 caactgcagc gatttcgtcg ccttttttgt attcacgaac tgcttcttcg cctgcaacgt
    17461 tccaggagat gtcagacagg tgaaccaggc cgtcgatgcc gccgtctagg ccgatgaaga
    17521 taccgaagtc agtgatagac ttgattttac cttcaacacg gtcgcccttg ttgtgggttt
    17581 ccgcgaactg ctgccacggg ttagctttgc actgtttcag acccagggag atacgacgac
    17641 gttcttcgtc gatatccaga accataactt ccactacatc gccaacgtta acaactttgg
    17701 acgggtggat gtttttgttg gtccaatcca tttcggaaac gtgtaccagg ccttcaacgc
    17761 cttcttcgat ttcaacgaag cagccgtagt cggtcaggtt ggtcacgcga ccagtcagtt
    17821 tggtaccttc cggataacgt ttagcgatag ctacccacgg atcttcgccc aactgtttca
    17881 ggcccaggga tacacgggta cgttcgcggt cgaacttcag cactttaaca gtgatttcgt
    17941 cgcccacgtt gacgatttcg ctcggatgct taacgcgttt ccaggccatg tcagtgatgt
    18001 gcagcaggcc gtcaacgccg cccagatcaa cgaatgcacc gtagtcagtg aggttcttaa
    18061 cgataccttt aacttccatg ccttcctgca ggttttccag cagctgatcg cgctctgcgc
    18121 tgttttcgga ttcgataacg gcacgacgag aaacaacaac gttgttgcgc ttctgatcca
    18181 gcttgattac tttaaattca agctctttgc cttccaggtg cagagtgtca cgcaccggac
    18241 gaacgtctac cagagaacct ggcaggaacg cacgaatacc gttcagctca acagtgaagc
    18301 cgcccttaac tttgccgttg ataacaccgg taacagtttc agcatcttcg taagcttttt
    18361 ccagcgtgat ccaggcttcg tgacgtttag ctttctcacg ggacagcaga gtttcaccga
    18421 agccgtcttc tactgcgtcc agagcaacgt caacttcgtc acctacctgg atttccagct
    18481 cgccctgggc gtttttgaac tgctcagccg ggatggcgga ctcagatttc agaccagcgt
    18541 caaccagtac tacgtctttg tcgatagcaa caacaacgcc acgaacgata gaacccgggc
    18601 gggtttcgat ttcttttaag gactcttcaa agagttgagc aaaagattca gtcatgttta
    18661 atcttcaggt ttatatttaa cgtccacctg gctccatgcc ggatggggtt gtttaacata
    18721 cccgctgtca ttccattgca acgggggtac tgcaaattcg gtcgcttatg cgagagccaa
    18781 tttctggcgc gcgtattgta gcgctttttc aatcacttgc tcaatgctta aggtggtgga
    18841 atccaacact aaagcatcgg ctgccggaac cagtggcgct accgctcggt tacgatcgcg
    18901 gtcgtcgcgt tctttgatct cggccaaaag gcgctcaaag ttaacactaa agcccttctc
    18961 ctgcaactgt agcatgcggc gatgcgcacg ttcttccgag gaggcgtcaa ggaaaatttt
    19021 cactggtgca tcagggaata ccaccgttcc catgtcgcgg ccatcggcaa tcagacctgg
    19081 taattcgcga aacgcgcgtt ggcgacgcaa taatgcttca cgaacgcgtg ggaatgtcgc
    19141 gacttgtgaa gccgcattcg ccacttcctg agtacgaatt tcgccgctga catcttcccc
    19201 ttcgaggatc acttccagat tgccattggt tgacacaaaa cgtacatcca gatgggatgc
    19261 cagcggtacc agcgcatctt ctgacgcaac atcaacatgg tgatgtaatg ccgccaatgc
    19321 cagtacgcga taaattgcac ccgaatccag cagatgccat tgcaatgctt ccgccatagc
    19381 cttacacaag gtgcctttcc ctgcaccgct tgggccatca atggtaataa ccggggcaat
    19441 tgccgtcatc tttatctcct taaaacaggc gtaccgttaa cataaccgcg cgcattatac
    19501 gcgccgacgc cacaaatcgt taacgttgtg tgcaaaaacc agttttgtct gtattcagat
    19561 ggggatttat tgcgcaatta tagcgggctg gcagaagacc agcccgaaag atgacaatga
    19621 tttacttacc agaggcgata cgatcacgga tgtgttgcgc acgctctgtt gatggtgggt
    19681 gagagtcaaa catggactgg gtgcgaccgc catccagact agccagtttt tcaaagctgc
    19741 caaccagccc ctaggtgctg atgccacgtt tcttcaacag atcaaaggag aaatcatctg
    19801 cttcggactc tttatcacgg gagtatttag cattaatagc gccttctgcg atatcaccca
    19861 attgtgagcg ggaaagctgg gaagccacac cgctggtagc tgaaatggca tcgcgtgcgg
    19921 caacgatcgc ataagaaact ttcatttcag ccagcgagtg acccaacgcg acgtggccca
    19981 gttcatggcc cagaacacct tcaatttcgt tgtcattcat catgtccatc aggccactgt
    20041 agacacggac acagccgttc gccatcgccc atgcgttgac gtcgctggtc atataaacct
    20101 atagttgacc ggcgtgccat taatattgtt acccaatgct ttggcgattt ttgccagacg
    20161 tttaccgtac ttgctggatt tacttgcgac ttgattgccg ctgtccattt cggcacagcc
    20221 ctgattggca atcgcttttg catcggcatc gcttaatgtt gctgctttat aggcatttaa
    20281 accggagctg atagccatat tggtatcaat accgtgggta ttttgacacc ctgtcagtaa
    20341 tgctgcagag gtcgcaatcg ccagcagtaa tttagtattc ttcatgtgaa tcatccgttg
    20401 attaatcata tataaacatt atgtatcaac aacttacatt gacaagcgac gctaccatta
    20461 atcaaaatga ctcacaaggt ccgaaaaaaa acgccgacaa tgtgc
//
LOCUS       DAJTOR010000064        20490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTOR010000064 DAJTOR010000000
VERSION     DAJTOR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.064"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..377)
                     /gene="nudI"
                     /locus_tag="OO249_002039"
     CDS             complement(<1..377)
                     /gene="nudI"
                     /locus_tag="OO249_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5636343.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEYGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYTWVKPEDLVHY"
     gene            656..1198
                     /locus_tag="OO249_002040"
     CDS             656..1198
                     /locus_tag="OO249_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636344.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARSTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1298..2500
                     /locus_tag="OO249_002041"
                     /pseudo
     CDS             1298..2500
                     /locus_tag="OO249_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
     gene            2720..3502
                     /locus_tag="OO249_002042"
     CDS             2720..3502
                     /locus_tag="OO249_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR5636345.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLSWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKSIACARDISRLLGWKSPFDLQAS"
     gene            3517..4722
                     /gene="rhmD"
                     /locus_tag="OO249_002043"
     CDS             3517..4722
                     /gene="rhmD"
                     /locus_tag="OO249_002043"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCR5636346.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4779..6068
                     /locus_tag="OO249_002044"
     CDS             4779..6068
                     /locus_tag="OO249_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5636347.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6086..6889
                     /gene="yfaU"
                     /locus_tag="OO249_002045"
     CDS             6086..6889
                     /gene="yfaU"
                     /locus_tag="OO249_002045"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR5636348.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6930..7832)
                     /locus_tag="OO249_002046"
     CDS             complement(6930..7832)
                     /locus_tag="OO249_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5636349.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8025..9215)
                     /gene="glpC"
                     /locus_tag="OO249_002047"
     CDS             complement(8025..9215)
                     /gene="glpC"
                     /locus_tag="OO249_002047"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5636350.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9212..10471)
                     /gene="glpB"
                     /locus_tag="OO249_002048"
     CDS             complement(9212..10471)
                     /gene="glpB"
                     /locus_tag="OO249_002048"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5636351.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLH
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLLCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10461..12089)
                     /gene="glpA"
                     /locus_tag="OO249_002049"
     CDS             complement(10461..12089)
                     /gene="glpA"
                     /locus_tag="OO249_002049"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5636352.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12362..13720
                     /gene="glpT"
                     /locus_tag="OO249_002050"
     CDS             12362..13720
                     /gene="glpT"
                     /locus_tag="OO249_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5636353.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13725..14801
                     /gene="glpQ"
                     /locus_tag="OO249_002051"
     CDS             13725..14801
                     /gene="glpQ"
                     /locus_tag="OO249_002051"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5636354.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14843..15085)
                     /locus_tag="OO249_002052"
                     /pseudo
     CDS             complement(14843..15085)
                     /locus_tag="OO249_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15264..15914
                     /gene="inaA"
                     /locus_tag="OO249_002053"
                     /pseudo
     CDS             15264..15914
                     /gene="inaA"
                     /locus_tag="OO249_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(15968..16222)
                     /gene="yfaE"
                     /locus_tag="OO249_002054"
     CDS             complement(15968..16222)
                     /gene="yfaE"
                     /locus_tag="OO249_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5636355.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16222..17352)
                     /gene="nrdB"
                     /locus_tag="OO249_002055"
     CDS             complement(16222..17352)
                     /gene="nrdB"
                     /locus_tag="OO249_002055"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5636356.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17499..19784)
                     /gene="nrdA"
                     /locus_tag="OO249_002056"
     CDS             complement(17499..19784)
                     /gene="nrdA"
                     /locus_tag="OO249_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5636357.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGECP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4810 a         4968 c         5506 g         5206 t
ORIGIN      
        1 tcataatgca ccagatcttc aggttttacc cacgtgtagt cctgaaactc ttcgtttatt
       61 ttcacttctc ggttggcaga aacgcagtca aaaatcaggt aaatcatata aatctcttcc
      121 ttgcgaccat ctgcatacgt cttggtgcga atatcatcgc tgaaggtcca cggcgtgatt
      181 tctgtcaaaa gcagctgttc tcccagttct tcgcgaattt cgcggcgtag tgcctcttca
      241 attcgttcgc catactccac gccgccaccc gaaagcgccc attgaccggg gaaaacgccg
      301 cgatcgtcgg ccattttgca cagcagataa gcaccatcat tttgaattaa agggcataca
      361 atagtccgtt gtcgcacagg ggtattcctt ataaataaaa agaaaccatg agaatacggt
      421 aactcagaaa tatgtgaaag tgacagtaat ctcttttgcg atgataactg cacttattat
      481 tatttttgtg tgtatcggta aagtgtgttt attttttcgg cgtttaatta tttaagaagt
      541 atttagttac aataagtgac tagatccctt ttttcgctca ttgaatcctc tgtgccaggc
      601 agtacaatcc cgcagctttg ggaattttga ttaattgctt gtgggaaagg ggataatgaa
      661 aaaaattgcg ctagcaggtc tggccggaat gctgttggtt tctgcatcgg tcaatgcaat
      721 gagcatcagc ggtcaggcgg gtaaagaata caccaatatt ggtgtcggtt ttgggactga
      781 atcgacgggc ctggcgttaa gcggtaactg gacacataac gacgacgacg gtgacgtcgc
      841 tggcgtgggg ctggggttga atctgcctct agggccgtta atggcgaccg ttggcggaaa
      901 aggcgtttac accaacccga attacggcga tgaaggttat gccgcagcgg taggaggtgg
      961 tttgcagtgg aaaattggca acagcttccg tttgtttggc gagtattact actctccgga
     1021 ttcactctcc agcggtattc aaagttatga ggaagcgaat gctggcgcgc gttccaccat
     1081 tatgcgtcca gtcagtattg aggcgggtta tcgctacctg aatttgtcgg gtaaagatgg
     1141 taaccgcgac aacgccgtgg ctgacggccc gtatgttggg gttaacgcca gtttctgatc
     1201 ccttccggcg cggcattctg tcgcgccatt cagtttctta ctacgcctta tctccctttg
     1261 cgttatagtg attcgataac aaaagcagga gataaccatg ttaaaagtgg aaatgttatc
     1321 caccggggat gaagtgttac acgggcaaat cgttgacact aacgctgcct ggctggccga
     1381 ttttttcttt catcaggggc tgccattatc tcgcctcaat acggtggggg ataaccttga
     1441 tgacttagtc accattcttc gcgaacgtag tcagcacgcc gatgtgctga tcgttaacgg
     1501 cgggctggga ccgaccagcg atgatttaag cgcactcgcc gctgcgacag caaaaggtga
     1561 aggcctggtg ctgcatgaag cctggctcaa agagatggaa cgctatttcc acgaacgtgg
     1621 acgagtaatg gcaccgagca accgtaaaca agcggagctg cctgccagtg ctgaatttat
     1681 caataacccg gtaggcaccg cctgtggttt tgccgtgcag cttaatcgtt gcctgatgtt
     1741 ctttactccc ggcgtaccgt cagaatttaa ggtgatggtc gagcacgaaa tcctgccgcg
     1801 cctgcgcgag cgtttttctt taccacagcc gccggtttgt ctgcgtttaa ctacctttgg
     1861 tcgttcggaa agcgatctgg cacaaagcct ggatactcta caactgccgc cgggcgtaac
     1921 aatgggctat cgctcttcaa tgccgattat cgaactgaaa ctcaccggac cggcaagcga
     1981 gcaacaggcg atggaaaaac tgtggctgga cgttaaacgt gttgccggac agagcgtgat
     2041 tttcgaaggc accgaaggac tgcccgtgca gatcagtcgc gaattgcaaa accgccagtt
     2101 cagcctgacg ttgagcgagt aattcaccgg tggtttattg gctttgcaac tttctcgcgc
     2161 aggtgctcca ttgctggcgt gtgaagtggt tccttcacag gaagaaaacc tggcgcaaac
     2221 tgcgcactgg attacagaac ggcgggccaa ccattttgcc gggctggcac tggctgtttc
     2281 gggtttcgag aacgaacatc tcaactttgc gctagccaca ccagacggca ctttcgctct
     2341 gcgtgtgcgt ttcagcacta cgcgctacag cctggctatc cgtcaggaag tgtgcgcaat
     2401 gatggcactg aatatgctgc gccgttggtt aaacggccag gatatcgcca gtgagcatgg
     2461 ctggattgag gttattgagt ccatgacctt atctgtctga ttccgccact ctgtgccggt
     2521 ttactgcacc ggcacattcc ccctccggca aaacttgtcc aacctgttgc actcaataag
     2581 aaaaaatccg tgcactgatt cacaaatttg tgcgtaattt ctccagtccg ctggacgagg
     2641 agcgggattt gatgcgcttc acaattttac tccccctgag caacgagact gattccagta
     2701 tctaagactg gagtccagta tgttggaatc aagcaaagtt cccgccctga cacgggctat
     2761 tgatattctc aatttgattg cccgcattgg cccctgtagt gctgcgacga tcattgacac
     2821 attgggaatc cctaaaagca cggcctattt gctgcttaat gaactcaggc gtcagcgttt
     2881 tcttagcctt gatcaccagg aaaatttttg tttgtggacc aggctggtgg agctttctgg
     2941 tcatgcgtta agcaagatgg atctccggga actggctcga ccgcgtttga cgcaactgat
     3001 ggataccacc gggctgttat gtcatctggg gattattgat aacggcagcg cttattacat
     3061 tttaaaagtg gagtcatcag ccactatcag cgtgcgttcc catgaaggaa aaagcctttc
     3121 gctttatcgc tccggaatag gtaaatgcct gctttcctgg caacctgcag cggtacagca
     3181 aagtattatc gaagggctgg tctgggagca ggcgacaccc acaacgatta ctcatccaca
     3241 gcaacttcac gaagaactgg cgcgtattcg ccgtcagggc tggagctatg acaacggcga
     3301 agattacccc gatgtccgtt gtgtcgctgc gccggtgttt aacgccaata acgagcttac
     3361 cgctgcgatc tcggtggtcg gtacccgctt acaaatcaat gaagaatacc gtgattatct
     3421 ggcgggcaag tccattgcct gcgccaggga tatttcacgt ctgttgggat ggaaaagtcc
     3481 ctttgactta caagcctcat aacaatggag aacatcatga ccctaccaaa aattaaacag
     3541 gttcgcgcct ggtttactgg cggtgcgaca gcagaaaaag gcgctggcgg cggtgattat
     3601 cacgaccagg gggcgaatca ctggatagac gatcatattg ccaccccgat gagtaaatac
     3661 cgcgattacg agcagtcacg ccagtcattt ggcattaacg ttcttggcac gttggttgtt
     3721 gaagtcgaag cagaaaacgg ccagaccgga ttcgctgttt cgacagccgg tgaaatgggc
     3781 tgttttatcg tcgaaaaaca ccttaaccgt ttcattgagg gtaaatgtgt cagtgatatc
     3841 aaactgatcc acgatcaaat gctcaatgcc accctgtatt actccggctc tggtggcttg
     3901 gtgatgaata cgatttcctg tgtcgatctg gctctgtggg atctgttcgg caaagtggtc
     3961 gggcttccgg tttataaact tttaggcggc gctgttcgtg atgagattca gttctacgcc
     4021 acaggtgcgc gtccggatct ggcaaaagag atgggcttta tcggtggcaa aatgccgacg
     4081 cactgggggc cacatgatgg cgatgcgggg atccgcaaag atgccgctat ggtcgcggat
     4141 atgcgtgaaa aatgcggtga agatttctgg ttaatgctcg actgctggat gagtcaggac
     4201 gtgaactatg cgaccaaact agcccacgct tgcgcgccct ataacctgaa atggatcgaa
     4261 gagtgcctgc cgccacagca gtatgaaggt tatcgcgaac tgaaacgcaa cgcgccagtc
     4321 ggaatgatgg tcaccagcgg tgagcaccac ggcacactgc aatcttttcg tacgctttca
     4381 gaaaccggta tcgacattat gcagccggat gttggctggt gcggtggttt aaccacgctg
     4441 gtggaaattg ccgcaatcgc caaatccagg gggcaactgg tggtgccgca cggttcgtct
     4501 gtttactctc accatgcggt gatcaccttc accaatacgc cattcagcga attcctgatg
     4561 accagcccgg attgttcaac gatgcgtccg cagtttgacc cgattctgct caatgagccg
     4621 gttccggtga atggtcgtat tcataaatca gtgcttgata aacccggttt tggcgtcgaa
     4681 ctcaatcgtg actgcaattt gaaacgcccc tacagccact aatcactccg ggcgttgctg
     4741 tgacaacggt aacgcccaca tattctaagg agctttttat gagcaccgct ttgcttgacg
     4801 ccgtggtgaa gaaaaaccgt gtgcgtttaa ttccgtttat gttggcgctg tatgtgctgg
     4861 cgtttctcga ccgttcgaat atcggttttg ccaaacagac ctaccagatt gataccgggt
     4921 tgagtaatga agcttatgcg ctgggagcag gcattttctt tgtggtatat gcgtttctgg
     4981 gtgttccggc gaatcttttg atgcgcaaac tgggggccag aacctggatt ggtacgacaa
     5041 cactgctgtg gggatttctt tcggcagcca tggcatgggc cgatactgaa gcgaaatttc
     5101 tgatagttcg cactctgctt ggtgctgcgg aggccggatt tttccctggt atgatttatc
     5161 tcacctcgca atggtttccg cagcgtaatc gcgccagcat tatggggctg ttctatatgg
     5221 gcgcaccgct ggcgttaaca ctgggatcac cgctttccgg cgcgctgttg gagatgcatg
     5281 gatttatggg gcatcccggc tggttctgga tgtttgtgat tgaaggattg ttggcagtcg
     5341 gtgctggggt attcacattc ttttggcttg atgacacacc ggagcaggca cgttttctga
     5401 gtaaacaaga aaaaacgttg cttatcaatc aactggcaag tgaagaacaa cagaaagtga
     5461 cttctcggct gagcgatgcg ctgcgtaatg gccgagtctg gcaactagcg attatctacc
     5521 tgaccattca ggtggcggtt tacggattaa ttttcttcct gccgacccag gttgcggcat
     5581 tgctgggaac aaaagtgggc tttacagcgt cggtggtcac cgccattccg tgggttgcgg
     5641 ccttgtttgg gacctggctt attccgcgct attccgataa aaccggcgaa cggcgtaatg
     5701 tcgcagcgct gacattactg gcggcgggca ttggtattgg tctgtccggg ctgctttctc
     5761 cagtaatggc gatcgtagcg ctgtgtgttg cagctatcgg gtttattgcc gtgcagccgg
     5821 tgttctggac gatgccgaca caactgcttt ccggtacggc gctggctgcg gggattggtt
     5881 ttgtaaacct gtttggtgca gtgggcgggt ttattgcccc gatcctgcgc gtgaaagcag
     5941 aaacgttatt tgccagcgat gcggcaggat tactgacact ggcagcggtg gcggtcatcg
     6001 gttcgctgat tattttcact ctgcgtgtaa atcgtactgt tgcgcagacc gacgtggcac
     6061 atcattaaat aggttaagga acacgatgaa cgcattatta accaatccct ttaaagaacg
     6121 tttacgcaag ggcgaagtgc aaattggtct gtggttaagc tcgactactt cttatatggc
     6181 agaaattgcc gctacttccg gttatgactg gttgctgatt gacggggagc acgcgccaaa
     6241 caccattcag gatctttatc accagctaca ggcggtagcg ccctatgcca gccaacccgt
     6301 gatccgtccg gtggaaggca gtaaatcgct gattaaacaa gttctggata ttggcgcgca
     6361 aactctattg atcccgatgg tcgatactgc cgatcaggct cgtcaggtgg cgtctgccac
     6421 gcgctatcct ccctacggtg agcgtggtgt tggggccagt gtggcacggg ctgcgcgctg
     6481 gggacgcatt gagaattaca tggcgcaagt caacgattcg ctttgtctgt tagtgcaggt
     6541 ggaaagtaaa acggcactgg ataacctgga cgagatcctc gacgtcgaag ggattgatgg
     6601 cgtgtttatt ggaccagcgg atctttctgc gtcgttgggc tacccggata acgccgggca
     6661 cccggaagtg cagcgaatta ttgaaaccag cattcggcgg atccgtgctg cgggtaaagc
     6721 ggctggtttt ctggctgtgg ctcctgatat ggcgcagcaa tgcctggcgt ggggagcgaa
     6781 ctttgtcgct gttggcgttg acacgatgct ctacagcgat gccctggatc aacgactggc
     6841 gatgtttaaa tcaggcaaaa atgggccacg cataaaaggt agttattgat atcaaaggcc
     6901 catggggatc ggctgtgggc ctgtgttaat tagtggttat tcgctgccag atcggcttcg
     6961 cttagctggg tggccgcgag cacctggtca cgatcaatgc cctgttccag catgcgcaaa
     7021 gcaattttaa tggcttgttc atgcatacct tcctgccagc caatttgatg cccttcctgc
     7081 cgtagccgtt cagcaatagt cattaatctc tccttttgta gtggtgaacg ttcggcaatc
     7141 tcctcaataa aacgggtgaa acgggaggta tcgccgcatt gcagcagata attaaacagt
     7201 gtttgtagct ggctgtcatt agtgaagcct ctaaccaaaa gcgtggtgat cctgtcgacc
     7261 atgccgatta aatcgcggtc gcgaatatgc ttttgaatca gttccagcag agctatacgc
     7321 cgatgttgca tgatctcatc gtcaggtacg atggtgatat ccaccaacgg aaaagcttcg
     7381 gtgtacaact gccgggcaag ttgcggatcg tcaaactcat ccagccagtt aagtgagtac
     7441 gggtagggcg atgtttcgcc atgataaaac agcaatggca ccaccagcgg aactctgtca
     7501 tagcctttat ccaggtgacg ctgcatggcg gcagtggcat agcgcattag ccgaaaagcc
     7561 atattctttt ctgcagagct ttgatgttca atcacgcagt agatatagcc gtcaccgtcg
     7621 ctggtttcca cggaccacaa aacatccgag tagtaagcgc gtaaactttt ttcaataaaa
     7681 ctagtgggtt ccaggcgtaa ggtttgcagg ttgcaaagct tgcgcagtgg ttctggtaaa
     7741 tgtatttcga gaaaatcccg tgcggtttcg ggtgtgaaca taaaggtttt aaataccgca
     7801 tcatgcgggg aggaggttgt tgattcggtc atggttgtcc gtcacctgat atttatgatg
     7861 acgcgacatt acggttatcc taggcctttg acatctgcca gtttcagcct ttgcgagacg
     7921 ttttcagagg aattttcact gcgttgcaac gcatacattg ggcacggaat cgtgtacgca
     7981 aaaatgcccg gtaaccacat gcttaccggg catcagaaag gagtttaagc cagcgcctgg
     8041 gccagtagcg taatcggatg ttcgcagcga agacttgtgg acatctcaat ctgccattta
     8101 caggtttcgc agtcggtgac caccagatct gcgccgcttt cttctatctg gcggaacagt
     8161 ggtgcgccga tggcttgtga ggtggggtag ttctcttttt tgaaaccgta agtacccgca
     8221 ataccgcagc actgggaatc cagcaccgtt aactcaagcc ccgggatttt acgcaacagc
     8281 tccagggtgt agagcgtcca gcccattttt tccatatggc acggagtgtg ataaaccact
     8341 ttcagcggca gcggtttcag cggtaacgtt ttgccttcgt ccagcttgcg ccacagccag
     8401 cgggttgcca gttcgatatg atcgcgcaag cctttgttgt cgacattcag cacttccggg
     8461 tattcgtcgc gcagggcaaa tgtacaggtt gaggaggtgg caatcaccgg aatgcctttt
     8521 actcccacag cttcgcggat cgactctaca ttcgtaattg cctgtttgcg tgctttatcg
     8581 gtaaagccgt tggcgattag cggtacgccg cagcattttt ctttgctgag cagttgtaca
     8641 ccggtaccca ttgcgttgag cactttaatt aaatctttac ctaactgcgg atggttgtag
     8701 ttaacgaagc agccgtgaaa gaaagcgacc tggtctttat attgtgcttg ctgagccgct
     8761 atgctgcgat accagcgacg gaacgtgccg aaggagtatt tcggtagcgt gcgacgatga
     8821 tcgattttta acgccgcatc aagcagctgc cgcaccggtt tcagcgaggt agcggtgttg
     8881 acgattggtg cgaacggcgt ggaaacgcta cccatcaggt cggtatgact caacacaaaa
     8941 ttacgcagcg acgggcgcgt ggtgtcatat tttgcccgcg cgcgctggat aatatcgcca
     9001 atcttcacat cggacgggca ggcgacttca caacgtttgc agttgatgca atatttcagc
     9061 gcctcgtcat acagtgcgcc atctttcaaa cgcagacgct cgccatccgg cccggcttgt
     9121 tttggccctg gataaccggg attcacccgg ctcaccgggc aggcggtggt gcagacggtg
     9181 cacttaatgc agttttcgaa gctggtgtcg ttcattgttg gcctcctgcg cgttgggcaa
     9241 tctgttgagc ggcatgtaaa gcactgacgg cacaaacacc gccgccgcat ccctgggcga
     9301 tgggatcaaa tccgcccagc accgaaccga tggcaaacag gttctcaatg gtttgccctg
     9361 cctgcgacgg gcgtagcgtc tcatcagtgg ttacaccgaa ctgctgccac ggttgcggcg
     9421 caaaaaaatc gcccttatac cattcacccc gcgtggcggt ttgtagcaca tcaaggccga
     9481 gaatcggctc gcgaatgccg ttacgttccg ctaccagtcc gccactaaag aaactgccgc
     9541 tggcgagaac cgcgaaatgt ggacgtagcg gaatatcggc gtgattgcgg gtccagattt
     9601 cattcactac gccattttta caggtcactt ttttcacttc atcgcccggc atccacacgc
     9661 caccctggcg cacaaactgg cgctgtaact ggttttgcag acgaatgccc agcacggaag
     9721 gcggcagcgt tggcaaaagc atcagtgaac aaagtagttt ttcattcaac caacgccaca
     9781 gtttgtcatc ggccaggccg aagcaggcag gcatcaggat catttcgcag gtattggcga
     9841 caggaataag cgcatcaagt aacagcggcc agttttcttc attatcaagg aaacgggcga
     9901 tattcacagc gcgaaattcg gtggcgttat tgcgcaggac atccagttcc ggcagctcta
     9961 tttctgcggt ttcaaccgaa agatccagtt cgtgcaacga agccgccgca agatgcgcct
    10021 gaaaatccat caggccgctt atcccgacca cacatatttt cttcgcgggc aacggccaga
    10081 cggggacttc tggcgaactt agccaggtag agcgcagagt gccgagcggc gtaatccgct
    10141 ggtgagccag ttctacgctg ccctgcaatt gcgcaccgct ttcagcgatt aatgcctgcg
    10201 cctggcaagc gagatcgagc acgcgttgtg gcccgagaag agagtaagga tgtgcaggtg
    10261 cctgctgacg caaagattcc agtccactgt gaatgtctgc cacgggttga ccatctggca
    10321 gatggctcag caaatccagc gaaccggatg agaaatgcaa cgcgctttga ccacgcgtga
    10381 caatggcaca gcgcaggccg tgtttttgca gttgcaggcc acagagtaat ccggcgaggc
    10441 cgccgcccat aatgacagta tcaaagcgca tctttctgct ccttctccag accacacagt
    10501 ccctgataaa cccagcgggt aaattcgctt tcgcgcagtg cgtctcccca ggcgatgggt
    10561 tgcacgcctt tccagcgttc gttaaggaag gtggaaagtt gctcgataga ttgcgcggac
    10621 gtcgtgacgt taaaccgttg cagcagtccg gcagcgcggc aggcgcagag ttcgccctgg
    10681 caggtgccca tccccacacg ggtacgacgg cgtaaatcca gcaggctatt aacgtttaaa
    10741 ttttctaccg cgtactgcac ttcacccgca gttaccgctt cgcactcaca taccaggcta
    10801 cggtgcagac ggccttcgct cagccaggca ggcgtgcgat cgccatgacg ataaaccgca
    10861 gaaccgcgca gcggggcagg cagggagatg actttacgca aggtaacttc agcgggttct
    10921 tgtgaaccag gcagtgccag atcggcagtc gtacaggggc gcgtgttgcc cagtttgcgg
    10981 cataccgcgt cggtagccca ttcagccatc agccgatagg tcatcagttt gccaccggtg
    11041 atggtgataa atccgtccag accatcgcgt tcagcatggt cgagcagcac gatgccacgg
    11101 ctgacgttac gtccgctcgg atcgtcatcg ctggcaacca gcgggcgcac gccagaatag
    11161 gcccgcaaaa tgcgcgtttt cgccatcacg ggggccagtt tttccccttc acgcagcaga
    11221 atatcaacct cttctgccgt cactcgatta tcgtcaatct cgttgtaatc aatacgtaaa
    11281 gaggtggtac caatcagcga aatggtatcg ccaggcacca gaatatcggc gtcggaaggt
    11341 ttacggcagc ggttgatcac atgctggtta atgcggtgat ccatgatcag cagcgatcct
    11401 ttcgccggga acatgcgaat gcgcagatcg gcatattcgg caatgtgttg cccccagatc
    11461 ccagcggcat taaccacgac aggtgcatga agggcctgag tttcgccggt gagatggtta
    11521 cgtacacgaa caccgcacac cgtcgcgcct tcacgaatca gccccgtgac ttcatgagcg
    11581 gtaaggataa cggcaccgtg ttctttggca tccagcatgt ttgctgcggt cagacgaaat
    11641 ggatcaacgg tgccatccgg aactttcacc gcgccaatca gtgccgggtt aacggcaggt
    11701 tcgataatgc gcgcttgctg cgggtctata gcttctgcgc tgatccctgc ttcttcgcag
    11761 gcgcgaataa aagtggcctg gaatgagagg tcatcttccg gcagggtgat aaacaggccg
    11821 ttggttggtt caacgcagtg acgtgcaatg cgtttcagga tctggttttc actaatgcat
    11881 tcgcgggccg attccgcatc ggttaccgca tagcgcgcac cgctgtgcag caggccgtgg
    11941 ttacgcccgg tggcaccggt tgcgatgtcg tggcgctcaa ccaaaatcac gcgcagcccg
    12001 cgcagggcac agtcgcgggc aatcccggct cccgttgcgc cgccgccaat gataatcacg
    12061 tcacttgatt gcgagtcgcg agttttcatt gttatccctc tgaagttcgt tttttaccat
    12121 ttagccatag taaaaacatg aattgtttga tttcgcgcat gttcgctcat aattcgaaag
    12181 tgaaacgtga tttcatgcgt cattttgaac attttgtaaa tcttatttaa taatgtgtgc
    12241 ggcaattcac atttaattta tgaatgtttt cttaacatcg cggcaactca agaaacggca
    12301 ggttctctca ctgaatcagg ctgttaatca taaataagac cacgggccac ggaggctatc
    12361 aatgttgagt atttttaaac cagcgccaca caaagcgcgc ttacctgctg cggagatcga
    12421 tccgacttat cgtcgattgc gctggcaaat tttcttgggg atattctttg gctatgcggc
    12481 ttactatttg gttcgtaaga actttgcgct tgctatgcct tatctggtcg agcagggatt
    12541 ctctcgcggt gatttaggtt tcgcgctttc ggggatctcg atcgcttatg gattttcgaa
    12601 attcataatg ggttcggtat cagatcgctc gaatccgcgc gttttcctcc ccgcaggttt
    12661 gattctggcg gcggcagtga tgttgtttat gggctttgtg ccatgggcga cgtcgagcat
    12721 tgcggtgatg tttgtacttt tgttcctctg cggttggttc caggggatgg ggtggccgcc
    12781 gtgtggtcgt actatggtgc actggtggtc gcagaaagaa cgtggcggca ttgtgtcagt
    12841 gtggaactgt gcgcacaacg ttggtggtgg tattccgccg ctgctgttcc tgctggggat
    12901 ggcctggttc aatgactggc atgcggcgct ctatatgcct gctttctgcg ccattctggt
    12961 ggcattattc gcctttgcga tgatgcgcga taccccgcaa tcctgtggct tgccgccgat
    13021 cgaagagtac aaaaatgatt atccggacga ctataacgaa aaagcggaac aggagctgac
    13081 ggcgaagcaa atcttcatgc agtacgtact gccgaacaaa ctgctgtggt atatcgccat
    13141 cgccaacgtg ttcgtttatc tgctgcgtta cggcatcctc gactggtcac cgacttatct
    13201 gaaagaggtt aagcatttcg cgctggataa atcctcctgg gcctacttcc tttatgaata
    13261 tgcaggtatt ccgggcactc tgctgtgcgg ctggatgtcg gataaagtct tccgtggcaa
    13321 ccgtggggca accggcgttt tctttatgac actggtgacc atcgcgacta tcgtttactg
    13381 gatgaacccg gcaggtaacc caaccgtcga tatgatttgt atgattgtta tcggcttcct
    13441 gatctacggt cctgtgatgc tgatcggtct gcatgcgctg gaactggcac cgaaaaaagc
    13501 ggcaggtacg gcagcgggct ttaccgggct gtttggttac ctgggcggtt cggtggcggc
    13561 gagcgcgatt gttggctaca ccgtggactt cttcggctgg gatggcggct ttatggtaat
    13621 gattggcggc agcattctgg cggttatctt gttgattgtt gtgatgattg gcgaaaaacg
    13681 tcgccatgaa caattactac aaaaacgcaa cggaggctaa tggcatgaaa ttgacgctga
    13741 aaaaccttag catggcgatc atgatgagca ctatagtcat gggaagcagt gcaatggcgg
    13801 cggacagcaa cgaaaaaata gtcatcgccc atcgcggtgc cagtggatat ttgccggagc
    13861 atacgctgcc agcaaaagcg atggcgtatg cgcagggagc ggattatctg gaacaggatt
    13921 tggtgatgac caaagacgac catctggttg ttctgcatga ccattatctc gatcgtgtta
    13981 ctgatgttgc cgatcgtttc ccggatcggg cgcgcaaaga cggtcgttac tacgcgatag
    14041 atttcacgct ggatgaaatt aagtcgttga aatttaccga aggtttcgat attgaaaacg
    14101 gtaaaaaagt gcagacttat ccggggcgtt tcccaatggg taagtccgac ttccgggtgc
    14161 acacctttga agaagagatt gaatttgttc aggggttaaa tcactctacc gggaaaaata
    14221 tcggtattta tccagaaatc aaagcgccgt ggttccatca tcaggaaggg aaggatattg
    14281 cggcaaaaac gctggaagtg ctgaagaaat atggttacac cggtaaagac gataaagttt
    14341 atttgcaatg ttttgatgct gatgagctga agcgtattaa gaatgagctg gaacccaaaa
    14401 tgggcatgga gctcaatctg gtacagctga ttgcctatac cgactggaat gaaacgcagc
    14461 agaaacagcc ggatggaagc tgggttaatt acaactacga ctggatgttt aagccgggtg
    14521 ccatgaaaca ggtggcggaa tatgcagatg gtattggtcc ggattaccat atgttgattg
    14581 aggagacatc gcagccgggt aatatcaaac tcactggcat ggtgcaagat gctcagcaga
    14641 ataaactggt agtgcatcct tataccgtgc ggtcagataa actgcctgaa tacactcctg
    14701 atgtgaatca gttatatgat gctctgtata acaaagcggg tgtaaatggg ctgtttactg
    14761 atttccctga taaggcagta aaatttctta ataaagagta atgttactgc aggatggcgt
    14821 tcatgcgcca tcctgtctca tatcaaattt ttccaatcgc cactaattgt tccatgcaca
    14881 tttgtagtaa aaacagtgga ctgccttcta ccggcttttc tttagccagc aacgaaatct
    14941 gtcgatagaa agttggttcg agaggaacgg aacacaattc acctgcaatg cttttcagcg
    15001 ttaactctgg ttgtaaggcg atgcctaatc cctgtcgaat aaaattcatc gctgtatccg
    15061 cctgattaaa ttcacatttt atctgctggg cgcactatta gtggtagtgg ctggtggaat
    15121 ctatgcgctg caaatgaagg gtaaaagcgg tgtcaagcta gtagtggcaa aagaaattca
    15181 ttaatacgac acgtttcatt aagattttcc tcaggtaacc acctatagtc attgtcgtta
    15241 ataaaaacga caggcgatag ggtatggcag tttcagcaaa gtacgacgaa tttaatcact
    15301 ggtgggcaac agagggcgac tgggttgaag agcctaacta tcgccgtaat ggtatgagcg
    15361 gggtgcaatg cgtcgagcgc aacggcaaaa agctgtatgt aaagcgcatg acgcatcatc
    15421 tgtttcattc tgtacgttat ccgttcggcc gaccaacgat tgtccgtgag gttgcggtaa
    15481 ttaaataact ggaacgggcc ggtgtcatcg tgccgaagat cgtttttggt gaagcggtga
    15541 aaattgaggg tgaatggcga gcgttgctgg tgactgaaga tatggcgggg ttcatcagca
    15601 ttgctgactg gtatgcccag catgcagtat cgccttattc tgacgaagta cggcaagcca
    15661 tgttgaaagc agtggcgctg gcgtttaaga aaatgcatag cattaatcgt cagcatggct
    15721 gttgttatgt tcgccatatc tatgtgaaaa cagaaggtaa ggcagaagct ggttttcttg
    15781 acctggagaa aagtcgtcgc cgcttgcgtc gcgataaagc gattaatcat gacttccgcc
    15841 agctggaaaa atatctggag ccaatcccca aagcggactg ggagcaggtg aaagcgtatt
    15901 actacgccat gtaatcgttg taggccagat aagacacata agcgttgtat ctggcactac
    15961 aaccaattca catctcgatt tcaatatcgc cttttgcccg gcaacaacag ggcaaaattt
    16021 cccccggctg aataaaggct aacggttcgg caatccagtc aacttgacct gcaaccaggc
    16081 gtgtgcgaca ggagccgcag taaccttcgc gacactggta ctcaaccgcc acattgtggg
    16141 attccagcgc tgccagaagg gaagggtgtt catcctggca cagcagttgt gtgccagtga
    16201 tgcgcagggt aacgcgggcc atcagagctg gaagttactc aaatcgtcgg tgtccacttc
    16261 cgagtcaatc tgcccgacca gataagaact gacttccact tcctgcggag caacctgcac
    16321 gttatcagac accagccaag tgttgatcca cgggatcggg ttggagcgcg tctggaacgg
    16381 cagatccaaa ccgactgcct gcatacggat attggtgatg tattcaacgt actggcagag
    16441 aatgtcttta ttcagaccaa tcatcgaacc gtcgcggaac agataatccg cccagtcttt
    16501 ctcctgttga gctgcctgaa caaacaggtc atagcactcc tgcttacact cttcggcaat
    16561 ttccgccatc tcaggatcgt ccgcgccgct gcgcagcaga ttcagcatat gctgggtgcc
    16621 ggtcaggtgc agggcttcgt cgcgggcaat caggcgaata attttggcgt tgccttccat
    16681 caattcgcgt tctgcaaagg caaaggaaca agcaaagctg acgtagaagc ggatcgcttc
    16741 cagcgcgtta acgctcatca ggcaaagata cagttttttc ttcagctcgc gcaggctaac
    16801 ggttacggtt ttaccgttaa cggtgtgggt gccttcgccc agaaggtgcc agtagctggt
    16861 catttcgatc agttcatcgt aatagctgga gatcccttcc gcacgtttct ggatctgctc
    16921 gttggtgacg atatcgtcaa acacaacaga cggatcgtta acgatattac gaatgatatg
    16981 agtataggaa cgggaatgaa tcgtttctga gaacgcccag gtttcgaccc aggtttccag
    17041 ttccggaata gaaataagcg gcaatagcgc cacgttcgga ctacgaccct gaatggaatc
    17101 cagcagcgtc tgatatttca ggttgctgat aaaaatgtgt ttttcgtgct ccggcagcgc
    17161 ctggtaatcg atacggtcgc gggagacgtc aacttcttcc ggacgccaga agaaagagag
    17221 ctgcttttcg atcagctttt cgaagatgtc atatttttgc tgatcgtagc gggccacgtt
    17281 gaccggctga ccaaagaaca tcggttcttt gagctgatca tttttcgtct gtgaaaaggt
    17341 ggtatatgcc atgagtgtgt cctgttggga gtttaatgcc ggataaggca ttttacgccg
    17401 catcctgtgc ctgatgcgac gctggcgcgt cttatcaggc ctacaaatcc gagccgtagg
    17461 ccggataagg cgttcacgct gcatccggca tctcaatatc agatcttaca tgcgccgctt
    17521 tcgcagccat cgtcctggat tgacggtacc agatcgtcct gcgcgtcttc agcgccgtca
    17581 cgggtgttct gataatacag cgttttgacc ccgaatttgt aggcggtgag caggtctttc
    17641 agcaactgct gcatcggcac ttttcctgac gggaaacgtg acggatcgta gttggtgtta
    17701 gcagagatcg actgatcgat aaatttctgc atgataccta ccagttgcag gtagccatcg
    17761 ttacccggca tttcccacag cagctcatag gcgtcgtgca ggtgctcgta gtccggcacc
    17821 acctggcgca aaataccgtc tttcgacgct ttgatgctga cgtaaccgcg cggcggttca
    17881 ataccgttag tggcgttaga gatctgcgaa gaggtctcgg acggcatcag ggcagaaagc
    17941 gtggagttac gcaggccgtg cgttttgatt gattcacgca atgcttccca gtcgtaatgc
    18001 agcggctcat tagcgatggc atccagatct ttcttatagg tatcgatcgg caggatccct
    18061 ttcgcgtaag tggtttcgtt aaaccacggg cactcgcctt gctctttagc cagctcatta
    18121 gacgctttca gcaggtaata ctgaatcgct tcgaaggttt tatgcgtcag gttgttggcg
    18181 ctgccgtcgg agtagcgttt accgtgcttc gccaggtagt aagcgaagtt gatcacacca
    18241 atacccagcg tacgacgacc catcgctcca cgtttggcgg ccgggatcgg gtaatcctga
    18301 taatccagca gcgcgtcaag tgcacgaacc gccagaattg ccagctcttc cagttcatcc
    18361 aggttattaa ttgcgcccag gttgaaagca gacagcgtac acagcgcgat ttcaccgttc
    18421 tcgtcgttga cgtcgttcag cggtttggtc ggcagggcta tctccaggca caggttagac
    18481 tgacgcactg gcgcgatggc cggatcaaac gggctatggg tattacagtg gtcaacgttc
    18541 tgaatataga tacgaccggt agacgcacgt tcctgcatca tcagcgagaa cagctcaacg
    18601 gctttcacac gctgcttgcg gatgctgtcg tctttctcat atttggtata cagacgttca
    18661 aactcttcct gatcggcgaa gaacgcgtcg tacagccccg gtacgtcgga cgggctgaac
    18721 agggtgatat cttcaccttt cagcagacgg gtatacatca gtttgttgat ttgtaccccg
    18781 tagtccatat gacgcacgcg gttgccttcc acaccacggt tgtttttcaa caccagcagg
    18841 ctttccactt ccagatgcca catcgggtag aacagcgttg ccgcaccgcc gcgcacaccg
    18901 ccctgagagc aggatttcac cgctgtctgg aaatgtttgt agaacggaat gcagccggta
    18961 tggaacgctt caccaccgcg aatcgggcta cccagcgcac gaatacgccc ggcgttgatg
    19021 ccgatcccgg cacgctggga aacgtattta acaatcgcgc tggaggtggc gttgatggaa
    19081 tccaggctgt caccgcactc gatcagtacg caggagctga actgacgagt cggggtacgc
    19141 acgccggaca tgattggcgt cggcagcgaa attttaaatg tggaaaccgc gtcgtaaaaa
    19201 cgcttcacat attgcaggcg cgtttcacgc gggtagttcg agaacaggca cgcggcaacc
    19261 agaatataaa ggaactgggc gctctcatag atttcgccgg tcacgcggtt ctgtaccaga
    19321 tatttgcctt ccagctgctt aacggcagca taagagaagg tcatatcacg gtcgtgatcg
    19381 ataaacgtgt ccatctgctt gaactcttct tccgtgtagt cttccagcag atgattatcg
    19441 tatttgccca tctcgaccat tttcaccacg tggtcgtaca gcgcaggcgg ctcaaactgg
    19501 ccgtaggctt ttttacgcag gtggaagatc gccaggcgcg cggcgagata ctgataatcc
    19561 ggcgcatcac gggagatcag gtctgcggca gccttgataa tggtttcgtg gatgtcagag
    19621 gtcttgatac cgtcataaaa ctgaatgtgg gagcgcagct cgacctggga aatcgaaacg
    19681 ttatgcagtc cttctgccgc ccaatccaga acgcgatgga ttttgtcgag attgatgcgc
    19741 tctgtgctac cgtcgcgctt tgtcaccagc agattctgat tcatgtatgt cgtacctgtt
    19801 tttggaattc tttccgcaat agtttcatgc agactacctg tagtacggga agctgcttag
    19861 aacactatat atagggggta tgtttgggat tcactgcaag atagtgtgaa aatggccctc
    19921 ttgcaagtgc ataactttgt ggataactca ggaaggaaaa agtggctttc gcgcacctta
    19981 ggtcagacaa ggtgtccggg aaagtcaatg ggaagagaaa aatttgttaa aaataactgt
    20041 tcggcgattt cttgaacggt agaataggtt ggataaattg atatagatgg tttattaatc
    20101 tttttgctaa tgattgcaaa aattgatcta agcacgagtc atcacataat ctgatgtatc
    20161 agttgtggtc aatatataag cgaatgtaag cgattgttga tgtcgaaatg gttgttttct
    20221 attcggttat tgttaaatgc gaagccattt tgttaattta ccttatatta taaaaaaatt
    20281 attcattact ttaatttagt ttaaatataa ctggtatccc ttaaggttat taacttgaat
    20341 tatgacgcac tgatattatt catcaaataa taacaaaata gccattgcat cgggttggac
    20401 aatttacaaa agaaagataa cccatattgt ataatgcgtt taaatatgcc gtcttatata
    20461 aaatgatggt cagattaata tgcggattat
//
LOCUS       DAJTOR010000065        20027 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTOR010000065 DAJTOR010000000
VERSION     DAJTOR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.065"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..309
                     /locus_tag="OO249_002057"
     CDS             <1..309
                     /locus_tag="OO249_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5636358.1"
                     /translation="IIVQWDANSNGIWDREPVKESDQIGFRLKEHVLETLRGATSCEG
                     KGWDKVTNPDAIIIDTFQVVRQDVSGFSPVLTVNMRAASKSEPQTVVNASYSVTGFNL
                     "
     gene            306..713
                     /locus_tag="OO249_002058"
     CDS             306..713
                     /locus_tag="OO249_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5636359.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            698..1021
                     /locus_tag="OO249_002059"
     CDS             698..1021
                     /locus_tag="OO249_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5636360.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            1034..4402
                     /gene="recC"
                     /locus_tag="OO249_002060"
     CDS             1034..4402
                     /gene="recC"
                     /locus_tag="OO249_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5636361.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRLWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDNI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            4578..7466
                     /gene="ptrA"
                     /locus_tag="OO249_002061"
     CDS             4578..7466
                     /gene="ptrA"
                     /locus_tag="OO249_002061"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5636362.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTTEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            7459..11001
                     /gene="recB"
                     /locus_tag="OO249_002062"
     CDS             7459..11001
                     /gene="recB"
                     /locus_tag="OO249_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5636363.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            11001..12827
                     /gene="recD"
                     /locus_tag="OO249_002063"
     CDS             11001..12827
                     /gene="recD"
                     /locus_tag="OO249_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5636364.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(12889..14220)
                     /gene="argA"
                     /locus_tag="OO249_002064"
     CDS             complement(12889..14220)
                     /gene="argA"
                     /locus_tag="OO249_002064"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5636365.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            14452..15705
                     /gene="amiC"
                     /locus_tag="OO249_002065"
     CDS             14452..15705
                     /gene="amiC"
                     /locus_tag="OO249_002065"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5636366.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15965..16144
                     /locus_tag="OO249_002066"
     CDS             15965..16144
                     /locus_tag="OO249_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR5636367.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKNMVCRSLSILQT
                     GFFMSEMSKKINSTN"
     gene            16141..16788
                     /locus_tag="OO249_002067"
     CDS             16141..16788
                     /locus_tag="OO249_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5636368.1"
                     /translation="MIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNAI
                     SRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILNN
                     YENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEKI
                     KQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDLILEEKLYLSIRIKVLMD"
     gene            16820..18400
                     /locus_tag="OO249_002068"
                     /pseudo
     CDS             16820..18400
                     /locus_tag="OO249_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            18400..19608
                     /locus_tag="OO249_002069"
     CDS             18400..19608
                     /locus_tag="OO249_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HCR5636369.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIQE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNLACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            19577..>20027
                     /locus_tag="OO249_002070"
     CDS             19577..>20027
                     /locus_tag="OO249_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR5636370.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFIRSHHSSLAISCDLSVEIPVKSEADNL"
BASE COUNT         4962 a         4664 c         5349 g         5052 t
ORIGIN      
        1 atcattgtgc agtgggatgc gaacagtaac ggtatctggg atcgcgaacc ggtaaaagag
       61 tccgaccaga ttggatttcg tctgaaggag catgtgctgg aaacgctacg cggtgcgaca
      121 tcctgtgaag gtaagggctg ggataaagtc actaatccgg atgccatcat tatcgacact
      181 tttcaggtcg tacgtcagga tgtcagcggc ttctcgccgg tgttgacggt taatatgcgt
      241 gctgccagta agtctgaacc gcaaaccgtg gtgaatgcca gctatagcgt gacaggattc
      301 aacctgtgaa ccgcgaaaag ggtgtttcgt cactggctct ggtcctgatg ctgctggttt
      361 tgggtagttt gctattacaa ggaatgagcc aacaggatcg cagttttgcc tctcgcgtga
      421 gcatggaaag tcagtcattg cgccgccagg ccatcgttca gtcggcgctg gcgtggggaa
      481 aaatgcactc ctggcagacg cagccagcag ttcagtgctc gcagtacgct gaaaccgatg
      541 cccaggtttg tttgcgttta ctggcagata atgaagcctt attgattgcc ggttatgaag
      601 gcgtttcgtt gtggcgaaca ggcgaagtca ttgatggaaa cattgttttt tcgccaagcg
      661 gctggagcga tttttgtccg ctgaaagaga gggcgttatg tcagcttccc tgaagaatca
      721 acaaggcttt agcctgccgg aggtaatgtt ggcgatggtg ttgatggtga tgattgtcac
      781 tgcgttatcg ggtttccagc gaacattgat gaacagtctt gccagcagaa accagtacca
      841 acagctctgg cggcatggct ggcagcaaac gcaactgcgc gcgatttcgc caccagctaa
      901 ctggcaggtc aaccgattgc agacatcgca ggcgggatgt gtcagcatca gcgttacgct
      961 agtttcaccc gggggcagag aaggcgagat gacccgcctg cattgcccga atcgtcagta
     1021 gtcaggagcc gctatgttaa gggtctacca ttccaatcgt ctggacgtgc tggaagcgtt
     1081 gatggagttt attgtcgaac gcgaacggct ggacgatcct ttcgaaccag agatgattct
     1141 ggtgcaaagt accggtatgg cacagtggct gcaaatgacc ctgtcgcaaa agtttggtat
     1201 tgcggcaaac attgattttc cgctgcctgc gagctttatc tgggatatgt tcgtccgggt
     1261 gttaccggag atccccaaag agagcgcctt taacaaacag agcatgagct ggaaactgat
     1321 gactctgctg ccgcaactgt tggagcacga agactttacc ctgttgcggc attatctgac
     1381 tgacgatagc gacaagcgaa aactgttcca gctttcctca aaagcggcgg acctgtttga
     1441 ccagtatctg gtctatcgcc cggagtggct ggcacaatgg gaaacaggac atttggttga
     1501 agggctggga gaagcacagg cctggcaagc gccgttgtgg aaggcgctgg tggaatatac
     1561 ccatgaactc ggacaaccgc gctggcaccg cgcgaatctc tatcagcgct ttatcgaaac
     1621 gctggagtcc gcgacgacct gcccgccggg gttaccttcg cgcgtcttta tatgcggtat
     1681 ttccgcgtta ccgcctgttt atctccaggc gctccaggcg ctgggtaaac atattgaaat
     1741 ccatctcctg tttaccaacc cctgccgtta ttactggggc gatattaaag atcctgctta
     1801 tctggcgaaa ctgctgactc gtcagcgtcg acacagtttt gaagatcgcg aattgccgct
     1861 atttcgcgat agcgaaaatg ccggacagct ctttaacagc gatggtgaac aggatgtcgg
     1921 caacccgctg ctggcctcat gggggaagct tgggcgcgac tacatttatc tcctttctga
     1981 cctggagagc agccaggagc tggacgcctt tgtcgatgtc acgccagata acctgctgca
     2041 taacattcag tctgacattc tggaactgga aaaccgtgcc gttgctggtg tgaacatcga
     2101 agagttttcc cgtagcgata acaaacgccc gcttgatcca ctggatagca gtatcacctt
     2161 ccacgtttgc catagcccgc agcgtgaagt tgaagttttg cacgatcgcc tgctggcgat
     2221 gctggaagaa gacccgacac ttactccgcg cgacatcatc gtgatggtgg ctgatatcga
     2281 cagctacagt ccgtttattc aggctgtgtt tggtagcgca cctgcggatc gttacctgcc
     2341 ttacgccatt tccgaccgtc gggcgcggca gtcgcatccg gtactggaag cgtttatcag
     2401 cctgttatcg ttgccagaca gccgctttgt gtcggaagac gtgctggcat tactggatgt
     2461 gccggtgctg gcggcgcggt ttgacatcac cgaagaaggg ctgcgttatt tacgcctgtg
     2521 ggtcaacgaa tccggcattc gttgggggat agatgacgac aacgttcgcg agctggaact
     2581 ccccgccacc ggacaacaca cctggcgatt tggcctgacg cgtatgttgc tgggctacgc
     2641 gatggagagc gcgcagggcg agtggcaatc ggttctacct tatgatgagt cgagcggctt
     2701 aattgcagaa ctggtggggc atctggcttc actgctaatg cagctaaaca tctggcgtcg
     2761 cgggctggca caggagcgtc cgctagaaga gtggttgccg gtttgtcgcg atatgctcaa
     2821 cgccttcttc ctgccggatg cggaaaccga agcggcgatg acgctgatcg aacaacaatg
     2881 gcaggcgatt atcgccgaag gtttaggggc gcagtatggc gacgcggtgc cgctgtcact
     2941 attgcgtgat gaactggcac agcgtctgga tcaagaacgt atcagccagc gttttctcgc
     3001 cggaccggtt aacatttgta ctctgatgcc aatgcgttca attccgttca aagtggtttg
     3061 cctgctggga atgaacgacg gcgtttatcc acgtcagctt gcgccattgg gctttgacct
     3121 gatgagccag aaaccgaagc gtggcgaccg tagccgtcgc gatgacgacc gctacctgtt
     3181 cctggaagcg ttaatttccg cgcagcaaaa actctatatc agttatatcg ggctttccat
     3241 tcaggataac agtgaacgtt tcccgtcggt actggtgcag gaactgatcg actacatcgg
     3301 gcaaagtcat tatctaccgg gcgatgaagc gctcaactgt gatgaaagcg aggcaagggt
     3361 aaaagcgcat cttacctgcc tccatacccg gatgccgttt gatccacaaa actaccagcc
     3421 aggcgaacga caaagctatg ctcgtgaatg gctacctgcg gccagccagg ctggtaaagc
     3481 acattctgaa tttgttcagc cgctgccgtt taccttaccg gaaaccgtgc cgctggaaac
     3541 gctacaacga ttctgggcac atccggtgcg ggcatttttc cagatgcgtt tgcaggtgaa
     3601 cttccgtacc gaagacagcg aaatccccga caccgagcca tttattctgg aagggcttag
     3661 ccgttatcaa atcaatcagc agttattgaa tgcactggtt gagcaggatg atgccgaacg
     3721 cttgttccgt cgcttccggg cggcagggga tttaccgtat ggcgcttttg gtgaaatttt
     3781 ctgggaaact cagtgccagg agatgcagca gcttgccgac agagtcattg cctgtcgcca
     3841 gccggggcag agtatggaaa ttgatctcgc ctgcaacggt gtgcagataa ctggctggtt
     3901 gccgcaggtg cagccggatg gcctgttgcg ctggcgtccc tctttattaa gtgtggcgca
     3961 gggaatgcaa ctttggctgg aacaccttgt ctactgtgcc agcggtggta atggtgaaag
     4021 tcgccttttt ctacgcaaag acggcgagtg gcgttttccg ccgcttgcag ccgaacaggc
     4081 tttgcattac ctctcacaac tgattgaggg gtatcgtgaa gggatgtccg cgccattgct
     4141 ggtgttacct gaaagtggcg gcgcgtggct aaaaacctgt tatgacgcgc aaaacgatgc
     4201 catgctggat gacgattcca cgttgcaaaa agcccgtacg aaattccttc aggcttacga
     4261 aggcaacatg atggtgcgtg gcgaaggtga taatatctgg tatcaaaggc tctggcggca
     4321 attaacacca gagacaatgg aggccatcgt tgaacagtcg caacgtttcc tgttaccgct
     4381 gtttcgcttt aatcagtcat gagtgctgta taaaaattgc gcaatctatc cgcttacttt
     4441 atgatgcgca ccagtcacgg actgatggtt atataaacat aggctgactc gtgcagcaca
     4501 agattaaatt ctggcagatg atttgcgtta acgtgttgaa tctggacaga aaattaagtt
     4561 gattatgagg tccgtgaatg ccccgcagca cttggttcaa agcattattg ttgttagttg
     4621 ccctttgggc acccttaagt caggcagaaa cgggatggca gccgattcag gaaaccatcc
     4681 gtaaaagtga taaagataac cgccagtatc aggctatacg tctggataac ggtatggtgg
     4741 tcttgctggt ttctgatccg caggcagtta aatcgctctc ggcgctggtg gtgcccgttg
     4801 ggtcgctgga agatcccgag gcgtaccagg ggctggcaca ttaccttgaa catatgagtc
     4861 tgatggggtc gaaaaagtac ccgcaggctg acagtctggc cgaatatctc aaaatgcacg
     4921 gcggtagtca caatgccagc actgcgccgt atcgcacggc tttctatctg gaagttgaga
     4981 acgacgcctt gcctggtgcg gtagaccgcc tggccgatgc tattgctgaa cctttgctcg
     5041 acaagaaata tgccgaacgt gagcgtaatg cggtgaacgc tgaattaacc atggcgcgta
     5101 cgcgtgacgg gatgcgcatg gcacaggtca gcgcagaaac cattaacccg gcacaccccg
     5161 gttcaaagtt ttctggtggt aacctcgaaa ctttaagcga taaacctggt aatccggtgc
     5221 agcaggcgct gaaagatttc cacgagaagt actattccgc caatctgatg aaggcggtta
     5281 tttacagcaa taaaccgctg ccggagttgg caaaaatggc ggcggacacc tttggtcgcg
     5341 tgccgaacaa agagagcaaa aaaccggaaa tcaccgtgcc ggtagtcacc gacgcgcaaa
     5401 agggcattat cattcattac gtcccggcac tgccgcgtaa agttctgcgc gttgagtttc
     5461 gcatcgataa caactcagcg aagttccgta gtaaaacgga tgaattgatt acctatctga
     5521 ttggtaatcg cagcccaggt acactttccg actggctgca aaagcaggga ttagttgagg
     5581 gcattagcgc caactccgat cctatcgtca acggcaacag cggcgtatta gcgatctctg
     5641 cgtctttaac cgataaaggc ctggctaatc gcgatcaggt tgtggcggca atttttagct
     5701 atctcaatct gttacgtgaa aaaggcattg ataaacaata cttcgatgaa ctggcgaatg
     5761 tgctggatat cgacttccgt tatccgtcga tcacccgtga tatggattac gtcgaatggc
     5821 tggcagatac catgattcgc gttccggttg agcatacgct ggatgcagtc aatattgccg
     5881 atcggtacga tgctaaagca gtaaaagaac gtctggcgat gatgacgccg cagaatgcgc
     5941 gtatctggta tatcagcccg aaagagccgc acaataaaac ggcttatttt gtcgatgcgc
     6001 cgtatcaggt cgataaaatc agcgaacaaa ctttcgctga ctggcagcaa aaagctgcca
     6061 atattgcgct ctccttaccg gagcttaacc cctatattcc tgacgatttc tcgctgatta
     6121 agtcagagaa gaaatatgac catccagagc tgattgttga tgagtcgaat ctgcgcgtgg
     6181 tgtatgcgcc aagccgttat ttttccagcg aacccaaagc tgatgtcagc ctgattttgc
     6241 gtaatccgaa agccatggac agcgcccgca atcaggtgat gtttgcgctc aatgattatc
     6301 tcgcagggct ggcgcttgat cagttaagca accaggcgtc ggttggtggc ataagttttt
     6361 ccaccaacgc taacaacggc cttatggtta atgctaatgg ttacacccag cgtctgccgc
     6421 agctgttcca ggcattgctc gaggggtact ttagctatac cgctacggaa gatcagcttg
     6481 agcaggcgaa gtcttggtat aaccagatga tggattccgc agaaaagggt aaagcgtttg
     6541 agcaggcgat tatgcccgcg cagatgctct cgcaagtgcc gtacttctcg cgagatgaac
     6601 ggcgtaaaat tttgccctcc attacgttga aagaggtgct ggcctatcgc gacgccttaa
     6661 aatcaggggc tcgaccagag tttatggtta tcggcaacat gaccgaggcc caggcaacaa
     6721 cgcaggcacg cgatgtgcaa aaacagttgg gcgctgatgg ttcagagtgg tgtcgaaaca
     6781 aagatgtagt ggtcgataaa aaacaatccg tcatctttga aaaagccggt aacagcaccg
     6841 actccgcact ggcagcggta tttgtaccga ctggctacga tgaatacacc agctcagcct
     6901 atagctctct gttggggcag atcgtacagc cgtggttcta caatcagttg cgtaccgaag
     6961 aacaattggg ctatgccgtg tttgcgtttc caatgagcgt ggggcgtcag tggggcatgg
     7021 gcttcctttt gcaaagcaat gataaacagc cttcattctt gtgggagcgt tacaaggcgt
     7081 ttttcccaac cacagaggca aaattgcgag cgatgaagcc agatgagttt gcgcaaatcc
     7141 agcaggcggt aattacccag atgctgcagg caccgcaaac gctcggcgaa gaagcatcga
     7201 agttaagtaa agatttcgat cgcggcaata tgcgcttcga ttcgcgtgat aaaatcgtgg
     7261 cccagataaa actgctgacg ccgcaaaaac ttgctgattt cttccatcag gcggtggtcg
     7321 agccgcaagg catggctatt ctgtcgcaga tttccggcag ccagaacggg aaagccgaat
     7381 atgtacaccc tgaaggctgg aaagtgtggg agaacgtcag cgcgttgcag caaacaatgc
     7441 ccctgatgag tgaaaagaat gagtgatgtc gccgagacac tagatccttt gcgcttgccc
     7501 ttacagggtg agcgcctgat tgaagcctct gccggcacag gcaaaacctt tacgattgcg
     7561 gcgctctatt tgcgcctgtt acttggacta ggcggttccg ccgcctttcc ccgcccgctg
     7621 accgttgaag aactgctggt ggtcaccttt accgaggctg ccacggcaga attgcgtggt
     7681 cgtatccgta gcaatatcca tgagttgcgc atcgcctgtc tgcgtgaaac taccgacaat
     7741 ccactgtacg aacgtctgct ggaagagatc gacgataaag cgcaagccgc gcagtggttg
     7801 ttgttagccg aacggcagat ggatgaagcg gcagtcttta ctattcacgg tttttgccag
     7861 cgcatgctca acctgaatgc ctttgaatcc ggcatgctgt ttgagcagca gctgattgaa
     7921 gatgagtctc tgctacgtta ccaggcctgc gccgatttct ggcgtcgcca ctgctacccg
     7981 ctgccgcgtg aaatagccca ggtcgtcttt gaaacctgga aagggccgca ggcgttgctg
     8041 cgcgatatta atcgttatct gcaaggcgaa gcgccggtta tcaaagcacc gccgcccgat
     8101 gatgaaacgc tggcttcccg ccacgcgcaa attgtggcgc gtattgctac ggtaaaacag
     8161 cagtggcgcg acgcagtggg tgaactggat gcgctgatcg aatcttctgg tattgatcga
     8221 cgcaagttta accgtagcaa tcaggctaaa tggatcgaca agatcagcgc ctgggcagaa
     8281 gaagagacca acagttatca gttgccggag tcgctggaaa aattctccca gcgtttctta
     8341 gaagatcgca cgaaagctgg gggggaaacc ccgcgacatc cactgtttga ggcgatcgat
     8401 caactgcttg cagaaccatt gtcgatccgc gatctggtga tcacccgcgc attggctgag
     8461 atccgcgaaa cagtagcgcg tgaaaaacgc cgccgtggcg aattgggttt tgatgacatg
     8521 ttaagtcggc tcgattccgc gctgcgtagc gaaagcggtg aggtgttggc agcggcgatc
     8581 cgtacgcgat tcccggtggc aatgatcgat gaatttcagg ataccgaccc ccagcagtac
     8641 cgaatttttc gccgtatctg gcaccatcag ccggaaaccg cattgttgct gattggcgac
     8701 ccgaagcagg ccatatatgc attccggggt gcggatatct tcacttatat gaaggcgcgt
     8761 agtgaagttc acgcccacta cactttagac accaactggc gctccgcacc tggaatggtg
     8821 aacagtgtga acaagctttt cagccagact gatgacgcgt tcatgtttcg cgaaataccg
     8881 tttattccag tgaaatcagc cgggaaaaat caggcgttac gttttgtatt taaaggtgaa
     8941 acgcagcctg cgatgaaaat gtggctgatg gaaggcgaaa gctgcggcgt tggcgactat
     9001 caaagtacca tggcgcaggt atgtgctgcg caaatccgcg actggctaca agccggacag
     9061 cggggcgaag cgttgctgat gaacggcgac gacgcgcgtc cggtgcgtgc ttcggacatc
     9121 agtgtgctgg tgcgcagccg ccaggaggcc gcccaggtgc gcgatgcctt aacgttgctg
     9181 gaaatccctt ccgtttacct ttcgaaccgc gacagtgttt ttgaaactct ggaagcgcag
     9241 gaaatgcttt ggttattgca ggcggtgatg acgcccgaac gtgagaacac cctgcgtagt
     9301 gcgctggcaa cgtcaatgat ggggctgaac gcgctggata tcgaaacgct gaataatgac
     9361 gaacatgcgt gggatgcggt agttgaagag ttcgatggtt atcggcaaat ctggcgcaaa
     9421 cgtggcgtta tgccgatgct gcgggcgctg atgtcggcgc gtaacattgc agaaaacttg
     9481 ctggcaacgg caggcggtga gcggcgtctt accgatatct tgcatatcag cgaactgcta
     9541 caagaagccg gaacgcagct ggaaagtgaa catgcgctgg tacgctggtt atcgcaacat
     9601 atcctcgagc cagacagtaa tgcctccagc caacaaatgc gcctcgaaag tgataaacat
     9661 ctggtgcaga ttgtcacgat ccataaatcg aaagggctgg aatatccgct ggtctggttg
     9721 ccatttatta ccaatttccg cgtccaggat caggcgtttt atcacgatcg ccactcgttt
     9781 gaggcagttt tggatcttaa tgctgcgcca aaaagcgtcg acctcgcgga ggtcgaacgt
     9841 ctggcggaag atctgcgttt gctttacgtg gcgctgacgc gttcggtttg gcattgcagt
     9901 ctcggcgttg caccgctggt gcgccgtcgt ggcgataaaa aaggtgacac cgacgtccac
     9961 caaagtgcgc tcgggcgttt gctgcaaaaa ggggaaccgc aagatgcggc agggcttcgc
    10021 acctgtattg aagcgttatg cgatgatgat attgcctggc aaacggcaca aactggtgat
    10081 aaccagccct ggcaggttaa tgatgcttta actgcagaac tgaatgcgag gacactacaa
    10141 cgattgcccg gcgataactg gcgtgtcacc agctactccg gcttgcagca gcgtggtcac
    10201 ggtatcgctc aggatctgat gccacggctg gatgttgatg ccgcaggcgt ggtcagcgtc
    10261 gttgaagaac cgacgttaac accgcatcag ttcccgcgcg gtgcgtcacc ggggacattc
    10321 ttgcacagtt tgtttgaaga cctcgatttt acccagccgg ttgacccgaa ctgggtacag
    10381 gaaaaactgg aactcggcgg ctttgaatcg cagtgggaac cggtgttgac cgagtggatc
    10441 acggctgtcc tccaggcacc tctcaatgaa aagggggtta gcctgagtca gctttccgac
    10501 cgcgataaac aggtggagat ggagttttac ctgccgatta gcgaaccgct catcgctagc
    10561 cagcttgatg cactaattcg ccagtttgat ccgctatccg ctggctgccc gccgctggag
    10621 ttcatgcagg tacgcggcat gttaaaaggc tttatcgacc tagtattccg ccacgaaggg
    10681 cgttattacc tgctcgacta taaatccaac tggctgggtg aagacagttc ggcttacacc
    10741 caacaggcta tggcagcagc gatgcaggca caccgctatg atctgcaata tcagctttat
    10801 accctggcgc tgcatcgtta tctgcgccat cgcattgctg attacgacta tgatcgtcac
    10861 tttggcggcg ttatctatct gttcctgcgt ggcgttgata aagaacatcc gcaacaaggg
    10921 atttacacaa cccgacccaa cgccgggttg attgccctga tggatgagat gtttgccggt
    10981 atgaccctgg aggaggcgta atgaaattgc aaaagcaatt actggaagct gtggagcaca
    11041 aacagctacg cccgctggat gtgcaatttg ccctgaccgt ggcgggagat gaacatcctg
    11101 ccgtcaccct cgcggcggca ctgttaagtc atgatgccgg agagggacac gtctgtttgc
    11161 cgctttcacg actggaaaat aacgaggcgt cgcatccgct gttggcgacc tgtgtcagtg
    11221 aaatcggtga gctacaaaat tgggaagaat gcttgctggc ttctcaagcg gtcagcaggg
    11281 gagatgaacc cacgccgatg atcctctgtg gcgatcgtct ttatttgaat cgcatgtggt
    11341 gtaacgagcg gacggtagcg cgcttcttca atgaggtgaa tcatgccatt gaggttgatg
    11401 aagctctact ggcgcaaacc ctggacaaac tttttccagt aagcgatgaa attaactggc
    11461 aaaaagttgc agcggcagtg gcgctgacgc ggcggatctc ggtgatttcc ggcggcccag
    11521 gcaccggtaa aacgaccacc gtggcgaagt tgctggcagc gttaattcaa atggccgacg
    11581 gcgaacgctg ccgtatccgt ctggctgcac caacgggtaa agctgccgcg cgcttaaccg
    11641 aatctctcgg caaggctttg cgacagttac cgctgaccga tgaacaaaag aaacgcattc
    11701 cggaagatgc cagcactttg caccgattgc tgggcgcgca gccgggtagc cagcgtttac
    11761 gtcatcatgc cggtaacccg ctgcatcttg atgtgctggt ggtagatgaa gcgtcaatga
    11821 tcgatctgcc tatgatgtcg agactgatcg acgccttgcc cgatcatgcg cgagtgatct
    11881 ttctcggcga tcgtgatcaa ctggcctcgg ttgaggctgg ggctgtgctg ggcgatatct
    11941 gcgcttatgc caacgcgggc tttaccgccg agcgtgccag gcagctaagc cgcctgacgg
    12001 ggactcacgt tccggcagga accggcacag aagcggcatc tttgcgcgac agtctctgcc
    12061 tgctgcaaaa aagctatcgt ttcggcagcg attctggcat tggtcagtta gctgcggcga
    12121 tcaaccgtgg tgataaaacg gcagtgaaaa ccgtttttca gcaggatttt actgatatcg
    12181 aaaaacgact tttacagagc ggcgaagatt atattgcgat gcttgaggaa gctcttgcgg
    12241 gttatggacg ttatctggat ctgctgcaag cgcgtgccga gccggattta atcattcagg
    12301 cgttcaatga gtaccaactt ttgtgcgccc tgcgggaagg gccgtttggc gtggctggac
    12361 tgaatgagcg aattgagcag tttatgcaac agaagcgcaa aattcatcgt catccgcact
    12421 ctcgttggta cgaaggtcga ccggtgatga ttgcccgtaa tgacagcgcg cttgggttgt
    12481 ttaatggcga tatcggtatt gcgctggatc gcgggcaggg gacgcgcgtc tggtttgcga
    12541 tgccggacgg caatattaag tctgtgcaac cgagtcgctt gccagagcac gaaactacgt
    12601 gggcgatgac ggtacataaa tcgcagggat cggagttcga ccatgcggcg ttgattttac
    12661 cgagccaacg cacgccggta gtaacgcgag agctggtata caccgcggtg acccgcgcgc
    12721 gtcgccgtct gtcgctgtat gccgatgagc gtatattaag tgcggcaatc gccactcgta
    12781 ctgagcggcg cagtggtctg gcggcgttgt ttagttcgcg ggaataaacg taattgccgg
    12841 atgcgacgca cgagtgttac gcatgtcgca tccgacaatt ttcatcgctt accctaaatc
    12901 cgccatcaac actttggatt tacgctggta gttgtacaac tgctttttgc tctcgggcag
    12961 taaatcaata tccaccgggg taaatccacg ttcctggaac cagtgaatac tgcgcgtggt
    13021 cagcacaaac aatttgctta agccgctctg cttcgcctga gcggcaatgc gttccagcag
    13081 aacctcgccc cgtgatgaac tgcggtaatc cgggtgaact gccacacagg ccatttcccc
    13141 aatcttctct tccgggaacg gatagagcgc ggcgcaggca atagtcgtgt tatcgcgctg
    13201 aataatggtg aatttgtcga tttccatctc cagctgctcg cgagaacggc gcaccagaat
    13261 accttgctgc tccagcgggc gaatcaactc cagaataccg ccaatatcgt tgattgttgc
    13321 gcgacgaatc tgctcggcgc tttccatcac aatctgcgta ccgataccgt cgcgtgagaa
    13381 caactcttgc aacagcgcgc catcttcctg ataactgatt aaatgacagc gacgcacgcc
    13441 gctgcggcag gctttcactg cgccacgcaa aaagcgcacc gtaccggagt tgtaatcgcc
    13501 tttctcttcc tgggcttcta cccgcgcttg cgcttcgtta gggaaaagtt ctgagacaat
    13561 atcaccgtcg tcattagtga cgccctgtga agagcaaaaa ccaatcatct tctcagcttt
    13621 cagtttgatg gccagttgag tggcaatctc ttccgaggtc agattaaagc tctcgccagt
    13681 gactgaaaca gcgaccggcc ccattagcac tattgcaccg ctgtccagtt gacgatggat
    13741 cgcgtcttca tcaatccgcc ggatacgccc gctatggcag taatccacgc cgtcatcgac
    13801 gcccagcggc tgggcaataa taaaattgcc actgacgacg ttgatatgcg cgccctgtag
    13861 cggcgtgtta ttgagactca tcgacaggcg agcagtgatg tccagttgca atgttcccgc
    13921 agcctgcttc accagttcca gtgttttggc gtcggtcaca cgtatattct tgtgatacag
    13981 cggttcgtgg tgatgcgcgg ccagatttgc gtcgatctgc ggacgtgcgc catagaccac
    14041 caccagacgg atgccgaggc tgtgcaacaa cccgatatca ttaacgatac tggagaaatt
    14101 ctcatgctca atggcttcac cgccgagcat gatgacaaac gtttttcccc ggtgggtatt
    14161 gatataggga accgaatggc ggaatccctc gaccagctcg gttttacgtt cctttaccac
    14221 ggcacacccc tttgcatgat tattctaaat tagtgtattt ttattctgtt ttttcgcagg
    14281 gtgcaagtgt aaattttatg cgagagcgac ttttttatca gtaatgccgt gaatataaaa
    14341 agaatgttta ccgtttatta gatgacagat tatgcgtctt tcgctaaagt ttccggtcaa
    14401 attagtcgtt tacttgttac acagcttaga tttcttttgc tcgggagagg catgtcagga
    14461 tccaacactg caatcagccg tcgtcgttta ctgcaaggcg cgggtgccat gtggctattg
    14521 agcgtaagtc aggtcagtct ggctgcggtc agccaggtcg tggcggtgcg cgtctggcct
    14581 gcgtccagct acacccgtgt gacggtagaa tcaaatcgtc agctgaaata taagcagttc
    14641 gcgttgagta accctgaacg cgtggtggtg gatatcgaag atgtaaacct gaactcggta
    14701 ctcaagggga tggcggcaca aattcgtgca gacgacccgt tcatcaagtc ggcgcgcgtc
    14761 gggcaatttg acccgcagac cgtacggatg gtttttgaat taaagcaaaa cgtaaaaccg
    14821 cagctgtttg cccttgcgcc ggtcgccggg tttaaagagc gtctggtgat ggacctctat
    14881 ccggccaatg cacaggatat gcaggacccg ctgctggcgc tgctggagga ttacaacaaa
    14941 ggcgacctcg aaaagcaggt gccgccagca caaagtggtc cacaaccggg taaagcaggg
    15001 cgcgatcgtc ctattgttat tatgcttgac cctggccacg gcggcgaaga ctccggtgcg
    15061 gtggggaaat acaaaacgcg cgaaaaagac gtggtattgc aaatagctcg ccgtctgcgc
    15121 tctctgatcg agaaagaggg caatatgaag gtgtacatga cgcgcaatga agacatcttc
    15181 attccgttgc aagtgcgcgt agcaaaagcc cagaaacagc gtgctgacct gtttgtctct
    15241 atccatgccg acgcctttac cagtcgtcag ccgagcggtt cctctgtgtt tgcgctctca
    15301 accaaaggtg caaccagtac tgcggcaaaa tatctggcac aaacccagaa cgcctcggac
    15361 ttgattggtg gcgtgagcaa aagcggtgac cgctatgtcg accacaccat gttcgatatg
    15421 gtgcagtcgc tgaccattgc cgacagcctg aagtttggta aagcggtact gaataagctc
    15481 ggtaaaatca acaagctgca taaaaatcaa gttgaacagg ccgggtttgc cgtactaaag
    15541 gcaccagata ttccctccat tctggtcgaa acggcgttta ttagtaacgt tgaggaagag
    15601 cgtaaactga aaacggcgac tttccagcag gaagttgcgg agtctattct tgcggggatt
    15661 aaagcgtatt ttgccgatgg ggcgacgctg gcgagaaggg gatgatgttt ttaatttatt
    15721 caatgaattt atgtttcatt gaataaatat tatttacata aatgttgaac gatggttttg
    15781 tgactgctgt cgaatatttg aatatcggct taaaaaataa gtctgtcatc tcttttatta
    15841 agcacaattc cgtgtaaggt atacgcatta gttaattatc ttgcaactgt taaatcaggc
    15901 aaggcaatgt ttgaagtaat tattacttct gacatgcctt gccttttttt tggggagcca
    15961 tggaatgatc atcgaaaaag tcatgaacaa taattgtgta caggcatcgg tgaacggaca
    16021 ggaggttatc atttctgggc ctggcgttgg ttacaataaa aatatggtat gtcggtccct
    16081 gagcatcctg caaaccggat tttttatgtc agaaatgagc aaaaaaataa actctacaaa
    16141 ttgattgagc atgtgggtat tgagtatatt tttgttgccg aaaagatagt gcattatgcg
    16201 gagagtaatc tcgaaaaaaa actcaatcca tcgctactac tgattcttgc tgatcacatt
    16261 tcgaatgcaa tatcccgagt cgtctcaggc atacaaatta ataatgtttt ccttgaggaa
    16321 atcaaagcgt tgtacaaagc agagtatgca ataagtcgtg atgcattaac aatcattaat
    16381 gagcaattta gcgttcaact accggatgat gagattggtt ttatagcatt gcatatttta
    16441 aataattatg aaaattcagt tgattatgaa tcagtaagga ttattgagct gtctcaaaaa
    16501 atcacggagc ttattgaagt tgtttataac agaagggtgg acagaagttc atttaactac
    16561 tccagattta tgatgcacct taaatatttt tctagtcgtg tgttatgtaa tgaaaaaata
    16621 aaacagaaaa atattggtga tatctatgag cagtttcttg aaaaagattt tcagttacaa
    16681 cgtgccattc atgaaattga aagatatctg tacgcaactt ttaaatatga tttgattctg
    16741 gaagagaaac tatatctttc tattcgcatc aaagtattaa tggactaatt atacataaca
    16801 ttttcatgag ataaccagta tgaaacaaaa gaaatcctgg agtttttttc agagcctggg
    16861 gaaggcattt atgtatccca tcgctctgtt aagtgtatgt ggtatgatgt tagggctggg
    16921 aagtggttta gccagcgatg atatggcaaa attcattcca gtgttggcta ttccgttaat
    16981 taaaaccata cttgatttta ttgttagcct tggtttgttt gccttcgtta atttacctgt
    17041 attgtttgcg atagctatac ctttgggatt attaaaagag aaagatgata aagcctatgg
    17101 cgctttttct ggcttaattg gctttatggc gatgcatctt ggaacgaact tttatcttaa
    17161 acagcacgac ttattggtag ctgctgacca aatgtcgaca catgggcaaa ccatcatact
    17221 cgggatccag tcctacaata ccagcgtgtt gggtgggatt gtggcagggt tattagtcgc
    17281 cagcatgtat aaaaagatcg ttaacttacg tattcctgaa taattagggt tttatagtgg
    17341 cccacgcctt gtgcctatca ttacactgat tgtgatgagt ggatttggtc tgatcattcc
    17401 ttttatctgg ccgccatttt tcaatctttt catgctcatt ggtcactgga tttcaacttc
    17461 cggtcctgtt ggttatttct tctatgctgt tgccgaacgc gtgacgatac cttttggctt
    17521 aaatcacttg gtgacatcag ttttccgctt taccccaatc ggcggatcgg cagtgattgg
    17581 cggtgaagag tattacggca ccctgaacat gtttatggca tacgtcaaag agaatgcggt
    17641 cattccgctg gatttggcgg ggaaaatgga gcagggcaaa ctgatgattc agtatggtct
    17701 ggctggagcc gcgctggcga tgtatcgcac tgctcatgct caaaacagaa gggctatcaa
    17761 agcattgctg atttccggtg tgctcacggt aattattggc ggcgtcagcg agccgattga
    17821 atttctgttc ttatttgtca gcccgctgtt gtttgtcttc catgccttta tgaatggatt
    17881 ggctaacatg gttctgccat atatgggcgt gaagatgggc tttactggcg atctgattca
    17941 atttattagc tttggtgtct tacgtggcac aagaacggga tggccattag cggtatgtgt
    18001 tgaagtggct tatttcttca tttattactt tgtgttccgt tggaccattc tcaaatttaa
    18061 cctgatgact gtaggccgtg aagagtccag tcctgtcacg ctgaacgctc acgaagaaac
    18121 tgcaatagct aacatcccag ttcatgataa atcagagttg caggcagcgg agcaaatggt
    18181 taaggctctg ggtggtaaag aaaatattaa atcactggat aattgtgtaa ctcgtttgcg
    18241 tttaactatc gcggatatgg gattgataaa tgaggctgca ataaaaaggg ccggaggaat
    18301 tgcggttgtg aaacttgatc aaaatacctt acaagtcatt atcggtacaa aagtcatcgc
    18361 cctacgtcgg gatatggata attacatggg gatatgttga tggatatttt taatacctca
    18421 atttctcgta aaggaacata ttgcacacaa tgggactttt gtgaagacag atttggtgta
    18481 aaagatgtat taccattctc tatttcagat atggatcttc cgatacagga agctatcatc
    18541 agaacattaa aaaaacgtct ggaacacccg attttagggt acagtcgttg gcagcatgat
    18601 gactatctga attcgatagt caattggtat catcaacaat ataaaacaga gattcaacca
    18661 gaatggatca cctatagccc aagcgtgatg tattcaatat ctaaggcaat agaaatattg
    18721 acattgaatg gagataatat tctcattttc acgcctgttt ataacgcatt ttttgatgtg
    18781 attaagcata atggtcgaaa catgctaaca acctctttga tgaaagaaaa tgggagaggc
    18841 tacgttatca actggcaaga ctttgagctt aaaattaaaa gcgcgaagat ggtgttatta
    18901 tgtaatcctc ataacccgac aggaacggtc tggtcggaag acgatcttca cagaatcgca
    18961 gcatgctgtg caaggcataa tatttggtta tgttcagatg aaatacatag tgattttgtt
    19021 tttaatcata gtttcacgtc agcgctaaca ataaaagatg acaaagtggt ggtgtttaat
    19081 tcgatttcga aaacatttaa tgttcctgcg ctaaccggat catatatgat ttcaactgac
    19141 gataatttta atcataagtt cagaacgata tcgaggtatc gcgatttcgt aaactcccca
    19201 tcagtactga atgtaattgc gaccattgtt gcctataatg aatgtgaaga atggttaaag
    19261 tcattaaagg tacatattgc ctctaacatt caatttacct tgcagtattt aaatgaaaat
    19321 attccggaat tgatcgtatg gccagctgag ggctgctatt tttcgtgggt tgattgctcg
    19381 gcccttggtt atccctttga taaattttac cgtcgattaa ttaacgaagg gagagtaggt
    19441 attatggcgg ggcatgttta tggtactgaa ggagagggct atctgcgtct taatctggcc
    19501 tgcagtcgag aaaaattata tatggggctt gcgcgccttg cgagtgtaat taaaaatatt
    19561 aatcaggaaa aagaaaatga acaatacgga tcttatccat cttattaaac gttttatgcg
    19621 taacgaacat aaagcagtag aagaggttat tgattccccc ctttcggaag tcgcgaattt
    19681 aattaaaata ttacaatctt gccagggaaa agttgttttt attggtgttg ggaaatctgg
    19741 cattattgcc agaaaacttg ctgcaacatt tgccagcacc ggaactccct cgttttttgt
    19801 tcacggtacg gaagcggtac acggcgacct tgggatggtg gcgaaagacg atgttgttat
    19861 tcttatttca aacagtggtg agacggcgga aatcctggcg acactgccta gtttgaaaaa
    19921 aatgggtaat tatttgatct cttttatccg cagtcatcac tcatcgcttg cgataagttg
    19981 tgatttatct gttgaaatcc ccgtcaaaag tgaggcggat aatttag
//
LOCUS       DAJTOR010000066        19875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTOR010000066 DAJTOR010000000
VERSION     DAJTOR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.066"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            54..830
                     /gene="eutC"
                     /locus_tag="OO249_002071"
     CDS             54..830
                     /gene="eutC"
                     /locus_tag="OO249_002071"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HCR5636371.1"
                     /translation="MTSESCALDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTG
                     RAGPRPRTQALLRFLADHSRSKDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDM
                     GRRLCAEAVEALKAQCVANPDVQVVISDGLSTDAITVNYEELLPPLMAGLKQAGLKVG
                     TPFFVRYGRVKIEDQICEILGAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEAD
                     RTCISNIHQGGTPPVEAAAVIVDLAKRMLEQKASGINMTR"
     gene            840..1499
                     /gene="eutL"
                     /locus_tag="OO249_002072"
     CDS             840..1499
                     /gene="eutL"
                     /locus_tag="OO249_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5636372.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1512..2012
                     /gene="eutK"
                     /locus_tag="OO249_002073"
     CDS             1512..2012
                     /gene="eutK"
                     /locus_tag="OO249_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5636373.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAAGRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            2058..3110
                     /gene="eutR"
                     /locus_tag="OO249_002074"
     CDS             2058..3110
                     /gene="eutR"
                     /locus_tag="OO249_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR5636374.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="OO249_002075"
     CDS             complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="OO249_002075"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5636375.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCNDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="OO249_002076"
     CDS             complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="OO249_002076"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5636376.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            5102..5527
                     /locus_tag="OO249_002077"
     CDS             5102..5527
                     /locus_tag="OO249_002077"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5636377.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5514..5963
                     /locus_tag="OO249_002078"
     CDS             5514..5963
                     /locus_tag="OO249_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5636378.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            6170..6745
                     /locus_tag="OO249_002079"
     CDS             6170..6745
                     /locus_tag="OO249_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5636379.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFRGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6841..7740
                     /gene="yfeX"
                     /locus_tag="OO249_002080"
     CDS             6841..7740
                     /gene="yfeX"
                     /locus_tag="OO249_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5636380.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="OO249_002081"
                     /pseudo
     CDS             complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="OO249_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
     gene            complement(9107..10531)
                     /gene="murP"
                     /locus_tag="OO249_002082"
     CDS             complement(9107..10531)
                     /gene="murP"
                     /locus_tag="OO249_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5636381.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="OO249_002083"
     CDS             complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="OO249_002083"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5636382.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            11595..12452
                     /gene="murR"
                     /locus_tag="OO249_002084"
     CDS             11595..12452
                     /gene="murR"
                     /locus_tag="OO249_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5636383.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCVEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            12581..13372
                     /gene="ucpA"
                     /locus_tag="OO249_002085"
     CDS             12581..13372
                     /gene="ucpA"
                     /locus_tag="OO249_002085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5636384.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            13676..14692
                     /gene="cysP"
                     /locus_tag="OO249_002086"
     CDS             13676..14692
                     /gene="cysP"
                     /locus_tag="OO249_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5636385.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            14692..15525
                     /gene="cysT"
                     /locus_tag="OO249_002087"
     CDS             14692..15525
                     /gene="cysT"
                     /locus_tag="OO249_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5636386.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            15525..16400
                     /gene="cysW"
                     /locus_tag="OO249_002088"
     CDS             15525..16400
                     /gene="cysW"
                     /locus_tag="OO249_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5636387.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            16390..17487
                     /gene="cysA"
                     /locus_tag="OO249_002089"
     CDS             16390..17487
                     /gene="cysA"
                     /locus_tag="OO249_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5636388.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            17621..18532
                     /gene="cysM"
                     /locus_tag="OO249_002090"
     CDS             17621..18532
                     /gene="cysM"
                     /locus_tag="OO249_002090"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5636389.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(18535..18903)
                     /locus_tag="OO249_002091"
     CDS             complement(18535..18903)
                     /locus_tag="OO249_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5636390.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            19008..19859
                     /gene="pdxK"
                     /locus_tag="OO249_002092"
                     /gene_synonym="thiJ"
     CDS             19008..19859
                     /gene="pdxK"
                     /locus_tag="OO249_002092"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5636391.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         4571 a         5044 c         5610 g         4650 t
ORIGIN      
        1 cggcccccgc gctgtcagaa gcaaagtgca ccaccaccac ctgtgcggca ccggtgacct
       61 cggaaagctg cgcgctggat ttaggttctg cggaagcaaa agcgtggatt ggtgttgaaa
      121 acccgcatcg cgcagacgta ttaacagaac tgcgtcgcag caccgtggcc cgcgtttgta
      181 ccggtcgtgc cggtccgcgt ccgcgtaccc aggcgttgct gcgtttcctg gccgatcact
      241 ctcgttcgaa agataccgta ctgaaagaag tgccggaaga gtgggtgaaa gcgcagggct
      301 tgctggaagt acgctccgaa attagcgaca aaaatctcta cctgactcgc ccggatatgg
      361 gccgccgcct gtgtgcagaa gctgttgaag cgctgaaagc acagtgtgtt gctaatccgg
      421 acgtacaggt cgttatttct gatggcctgt caactgatgc catcaccgtg aactacgaag
      481 agctcctgcc gccgctgatg gcgggcctga aacaggccgg gctgaaagtc ggcacgccat
      541 tctttgttcg ttatggtcgc gtgaagattg aagatcagat ctgtgagatc ctcggcgcga
      601 aagtggtgat cctgctggtg ggggaacgtc cggggttggg tcagtcagaa agcctctcct
      661 gttacgccgt ttactcgccg cgtatggcga ccaccgttga ggccgatcgc acctgcatct
      721 ctaacattca ccagggcggc acgccgccgg ttgaagccgc agccgtcatt gtggatttgg
      781 cgaaacgtat gctggagcag aaagcatccg gcatcaacat gacccgataa ggaggcatca
      841 tgccagcttt agatttgatt cgaccgtcgg taaccgccat gcgggtgatt gcctccgtta
      901 acgctgaatt tgcgcgtgaa ctaaaattgc cgccgcatat tcgtagcctc gggctgattt
      961 ctgctgattc cgatgacgtc acctacattg cggctgacga agcgaccaag caggcgatgg
     1021 tcgaagtggt atatggccgc tcgctgtatg cgggagcagc acacggaccg tcaccgactg
     1081 ccggtgaagt gctgattatg cttggtgggc caaacccggc ggaagtgcgt gctggtctgg
     1141 atgcgatggt tgcgcatatt gaaaatggcg ctgctttcca gtgggctaat gacgcagaaa
     1201 acaccgcatt cctggcacat gtagtttcgc gtaccggttc ttatctctca tcaaccgccg
     1261 ggatcacgct tggcgatccg atggcgtatc tggtggcacc gccgctggaa gcgacctacg
     1321 gcattgatgc agcgttgaaa tctgccgacg tacagctggt gacctatgtc ccgccaccgt
     1381 ctgaaaccaa ctactcggca gcatttttaa ccggtagcca ggccgcgtgt aaagcagcct
     1441 gtaacgcctt taccgatgca gtgctggaaa tcgcgcgtaa tccaatccag cgtgcgtaac
     1501 ggaggttgcc gatgatcaat gcactgggat tgctggaagt ggacggcatg gtcgccgcga
     1561 tagatgcagc ggatgccatg ctcaaagcag ctaacgttcg tctgctcagt cacgaagtgc
     1621 ttgaccctgg tcgcttaacg ctggtggtgg aaggcgatct ggcggcgggt cgtgcggcgc
     1681 tggacgcagg ttgtgctgcc gcgatgcgta ccgggcgtgt catcagccgc aaggagatcg
     1741 gtcggccaga cgatgacacc cagtggctgg ttactggctt taaccgccag ccgaagcaac
     1801 ccgtaaggga acccgacgcg ccagttatcg tcgcggaatc tgctgacgag ttgttggcgc
     1861 tgttaacatc agtacgtcag ggaatgacgg caggagaagt ggctgcccac tttggctggc
     1921 cgctggaaaa agccagaaat gcgctcgaac agctcttttc tgccgggacg ttacgtaaac
     1981 gcagtagtcg ttatcgtctc aagccccatt aacctgtcgg aggtgctggg tgtcctacaa
     2041 cacccggcat taacatcatg aaaaagaccc gtacagccaa tttgcaccat ctttatcatg
     2101 aacccttacc cgaaaacctg aagctcacgc cgaaggtcga agtggataat gttcatcaac
     2161 gacagacaac ggatgtctat gaacatgctt taacgattac cgcctggcag cagatttacg
     2221 atcagctgca tccgggcaag tttcatggtg aatttacgga aattctactc gatgatattc
     2281 aggtttttcg tgaatacacc ggtctggcgc tgcgtcagtc gtgcctggtc tggccgaact
     2341 cgttctggtt tggcattccg gcgacgcgcg gtgagcaggg atttatcggt tcgcaatgtc
     2401 tgggaagcgc ggaaatcgcc acccgccctg gtggcactga atttgaactg agcacgccgg
     2461 atgattacac gatcctgggc gtggtgcttt ctgaagatgt catcacccgg caggctaact
     2521 ttttgcataa cccggatcgg gtattacata tgctgcgtag ccagtcggcg ctggaagtga
     2581 aagagcagca taaagccgcg ctgtggggct ttgtccaaca ggcgctggcg acgttttgcg
     2641 agaacccgga aaatctccat cagcctgcag tgcgaaaagt gctgggggat aatttgctaa
     2701 tggcgatggg ggcgatgctg gaagacgcgc aaccaatggt gacggcggaa agcatcagtc
     2761 atcagagtta ccgtagatta ctttcccgcg cccgtgaata tgtgctggaa aatatgtctg
     2821 agccggtgac ggtgctggac ttgtgtaatc aactgcatgt cagtcgccgc acgctacaaa
     2881 acgcgtttca cgctatttta ggcattggcc caaacgcgtg gctgaaacgc attcgcctga
     2941 acgccgtacg ccgcgaactg ataagcccgt ggtcgcaaag cacaacggta aaagacgccg
     3001 ccatgcagtg gggattctgg catctggggc aatttgtcac ggattaccag cagctgtttg
     3061 ccgagaagcc gtcgttgacg ttgcatcagc ggatgcggga atgggggtga gggagttaca
     3121 cccaatccct gaccttaata aactcactta acgccgcttc tgggctgcca tcttttggct
     3181 gataatcata ctcccagcgt accagtggcg gcattgacat caggatcgac tcggtgcgcc
     3241 cgccagtttg caggccaaac agagtgccgc gatcccagac cagattgaac tcgacataac
     3301 gaccgcgacg gtagagctga aaattgcgct cgcgctcgcc gtaggccatt gctttacgtc
     3361 gctctacaat tggtaaataa gcgtcggtgt agcctttgcc taccgcctgc ataaaggcaa
     3421 aacagtggtc gaaatctggc gtgttcagat catcaaagaa cagcccgcca ataccgcgct
     3481 gttcgttgcg atgtttgagg tagaagtaat cgttgcacca ctttttgtaa cggggataaa
     3541 cgtcttcacc aaatggcagg cacaggtcac gggcggtgcg gtgccagtga atggcgtctt
     3601 cttcaaaacc atagaaaggg gttaaatcga agccgccgcc aaaccaccag acgggctctg
     3661 cacccggttt ttcggcaata aaaaaacgca cattcgcgtg gctggtggga acatacgggt
     3721 tatgcggatg cactaccagt gaaacgccca tcgcctcgaa actgcgcccg gcaagttccg
     3781 ggcgatgagc ggtggcggaa gcaggcatcg cctcgccgtg gacgtgagaa aagttgacgc
     3841 ctgcctgttc gaaaacacca ccattacgca acacccgact acgcccgccg ccgccagctt
     3901 cgcgctgcca actatcttcg acaaattctg cgccatcgac ggcggtcagc tgctgacaaa
     3961 tcgtatcctg aaggttgagc agaaactgtt taacctggtg tgcgtcgggt ttcataactt
     4021 atcgcttttt cgaatgtgct ttctggttgt cgaaccagtg gaaataactg atcacgcctt
     4081 cagcaatcgc tgtggcgatt ttctgacgaa acgccgccgt gcctaacagc cgctcttctt
     4141 ccgggttggt gataaacgag gtttccacca gcaccgaagg aaccgacggc gatttcaaca
     4201 ccacaaacgc ggcttgttcg gtgttgcggc tgtgcagttt atgcaccggc ttaatctttt
     4261 tcagaatatg cgagccaagc gtcaggctgt ttttaatagt atcggtttgc accagatcaa
     4321 acagcacttg ctgcaatagg tgatccttgt cagtcgcctt tttaccggca acttcatcgg
     4381 cgcggttttc gcgttcagac aggtatttcg ccattgcgct acttgcccca cggttagaga
     4441 gggcaaatac cgaagcaccg gcagctttcg ggttggtaaa gccatcggca tgaattgaca
     4501 taaacagatc tgcgccatgt ttatgggcga tttcaacgcg atcgtaaagt gggataaacg
     4561 tatcgccaga acgcgttaaa cgcgcatcaa tcccatgatt acgcaaaatg gaacggacgt
     4621 ttttagcaat cgccagcacc acatgttttt ctttcgaacc gttgcgtccg atcgctccgg
     4681 tatcaattcc gccgtggcct ggatcgagaa caacgacacg tttgccgcca gattttttgg
     4741 ctttcggctt gctgtgtccg ttgctggttt ttaaaggttc gtctttggcg atggcttgcg
     4801 acattcctga caacgtcagg gcagccaaac cggctttcag cacctggcgg cgcgaagtga
     4861 gtgtttttag tggtttaaaa gtgctcatac ggcctgagtt gtaataataa agttccagat
     4921 gttatatcgt atcgcgtaat ccgttacgac ggtttgatgt tgagaattgt tttacttttc
     4981 atttcaatag ttgacaactc cccattatgc cactttttca accgattttc gtcagatatt
     5041 ggctaattct accgttcacg ccataatcag ccttttaacc cgcaaaaagg taacgaatac
     5101 catggagata cgcgtatttc gccaggaaga tttcgaagag gtcatcaccc tttgggagcg
     5161 ttgcgacttg ctgcgtccgt ggaacgatcc ggaaatggac atcgagcgta agatgaacca
     5221 tgacgtcagt ttgtttttgg tcgctgaggt gaacggtgaa gtggtcggaa cggtgatggg
     5281 cggttatgac gggcatcgcg ggtctgctta ttatcttggc gtgcatccag agtttcgtgg
     5341 gcgtgggatt gccaatgcgt tgcttaatcg gctggagaaa aagctgattg ctcgtggctg
     5401 cccgaaaatt cagatcaatg tgccggaaga taacgacatg gtgctcggaa tgtatgaacg
     5461 cctgggatat gaacacgccg acatgctgag tctgggtaag cgtttgattg aagatgaaga
     5521 gtactgagtt ccatcctgtc cattatgatg cgcacggacg cctgcgttta cccttgctct
     5581 tctggcttgt gctattgctt caggcgcgaa cctgggtgct gtttatcatt gccggtgcgt
     5641 cgcgcgagca gggtactgcg ctgctaaatc tgttttatcc cgatcacgat aatttctggc
     5701 tggggttgat tcccggtatt ccggcggtgc tggcgttttt gttgagcggt cggcgggctt
     5761 cgtttcctcg cacctggcat gtgctctatt ttctgttgct gttggcgcag gtggttttac
     5821 tttgctggca accgtggctg tggctgaacg gtgaatccgt tagcggtatc ggtctggcgc
     5881 tggtagtggc ggatattgtg gcgttaatct ggctgctgac caatcgacgt ttacgcgctt
     5941 gttttaatga agaaaaagaa taacgcagag gtgcgctttg tttatgccgg atgcggcgtg
     6001 aacgccttat ccggcctaca aaacatcgaa aattcaatat attgcaggag aaagtaggcc
     6061 tgataagcgt agcgcatcag gcaattttgt gtttgtcatc aacctttaac ggcacttttt
     6121 ggtgaatttg cactccaagc aacgttattg aataaccaaa ggaagtgaca tgaaatcgct
     6181 gcgtttaatg ttatgcgcta tgccgttgat gctgaccggc tgttccacga tgtcgtcagt
     6241 taactggtcg gctgctaacc cgtggaactg gtttggatcg tccaccaaag tgagcgagca
     6301 gggcgtgggt gaattaacgg cgtccacacc actgcaagaa caagccattg ctgatgcgct
     6361 tgatggcgat tatcgcctgc gcagcggaat gaaaaccgcg aacggcaacg tggtgcgctt
     6421 ttttgaagtg atgaaaggcg acaacgtggc gatggtgatt aacggcgatc aaggcacgat
     6481 cagtcgcatt gacgtgctgg atagcgatat tcctgctgac accggtgtta aaatcggtac
     6541 accgtttagc gacctttaca gcaaagcatt tggcaattgc caaaaagctg atggtgatga
     6601 taatcgtgcc gtcgaatgta aagccgaagg cagtcaacat attagctacc agttcagagg
     6661 ggaatggcgt ggtcctgaag ggttaatgcc ttcggacgat accctgaaaa actggaaagt
     6721 cagtaaaatt atctggcggc gttaatttgc gtctgaacaa accgccgccg cgaaaaaaca
     6781 cgtaaaatag cgctcaacaa tgccacggat tgcgtggcat tcttattttc aggaggaaca
     6841 atgtctcagg ttcagagtgg cattttgcca gaacattgcc gcgcggcgat ttggatcgaa
     6901 gccaacgtga aaggggaagt tgacgccctg cgtgcggcca gtaaaacatt tgccgacaaa
     6961 ctggcaactt ttgaagcgaa attcccggac gcgcatcttg gtgcggtggt tgcctttggt
     7021 aacaacacct ggcgcgctct gagcggcggc gttggggcag aagagctgaa agattttccg
     7081 ggctacggta aaggccttgc gccgacgacc cagttcgatg tgttgatcca cattctttct
     7141 ctgcgtcacg acgtaaactt ctctgtcgct caggcggcga tggaagcctt tggtgactgc
     7201 attgaagtga aagaagagat ccacggcttc cgttgggtgg aagagcgtga cctgagcggc
     7261 tttgttgacg gtacagaaaa cccggcgggt gaagagacgc gccgcgaagt ggcggttatc
     7321 aaagacggcg tggatgccgg cggcagctat gtgtttgtgc agcgttggga gcacaacctg
     7381 aagcagctca accggatgag cgttcacgat caggagatga tgatcgggcg caccaaagag
     7441 gccaacgaag agattgacgg cgacgaacgt ccggaaacct ctcacctcac ccgcgttgat
     7501 ctgaaagaag atggcaaagg gctgaagatt gttcgccaga gcctgccgta cggcaccgcc
     7561 agtggcactc acggtctgta cttctgcgcc tactgcgcgc gtctgcataa cattgagcag
     7621 caactgctga gcatgtttgg cgataccgat ggtaagcgtg atgcgatgtt gcgcttcacc
     7681 aaaccggtaa ccggcggcta ttacttcgcg ccgtcgctgg acaagctgat ggcgctgtaa
     7741 gcgtttctga ttctatgatt aaaggccagc cagtaacaaa actgactggc ctttttatta
     7801 cttttgcttc aacgccgcat acacctgatc aactacccaa ccataagttg caatcggtaa
     7861 ctgaccgctt tcgaacatat tgggattttt ttgtggatcg gcaactggcg aatggggctt
     7921 gttgcttaac atcacaatcg ccatatgatt gaccggatcg ataacggtta ctgtcccggt
     7981 ccagccagtg tgaccgtagg tttgcgggct tgccagcgtg ccaaacgtcg gcgtcatggt
     8041 ggcattacca ttcacgcgcc agccgaggcc aaaagtggca tcttccttag agctggtggt
     8101 gaacatcttc accgtttccg cactgaacag ctgcacatcg ccatagcccc cgccgttcag
     8161 catcgtttgc attaacaccg caatatcgcc ggtattggaa aacaaacctg cgtgcccgga
     8221 aacgccgccc atcgaataga aggctttttc atcgtgcacc tgaccccaga gagtggaggt
     8281 gcggatattc gggaaatgga tcacgccatc gcgggtattg ccgttcaatt ccgtggcggc
     8341 aatttgttgc ggtttaaagc ctttcagcag cgggttaaat accgtatgtg tcaggccgag
     8401 cgggcgataa atcgactctt caacatagcg gtcaagcggc tgaccggtaa ccgactcgac
     8461 gataaatcca agcagcatat aatcgacatc gctgtagata tgtttgctgc cgggctgata
     8521 ttccagcggc gtgcgcttga tcatctccag cgtctggcct ttatcctagg aatataacgc
     8581 gcccgcgacc gctttattcg ggtattgcgg atctgccggg aaaccgccgc tgtgatgcag
     8641 caggtcagaa atccgcaggg tgtttttgcc tttaatggtg tcatttggac tatcggcaaa
     8701 ccccggaata tatttcgcaa tccgatcgtc aggatgcagt ttgccttcgc tcatcagctt
     8761 ttgcagggcg aagttcgtgg cgtacatttt ggtgtttgag gccaggtcat acagcgtccc
     8821 ggtggtggct ttgacaggct gttccatcaa cacgctgcca tcgtactttt tcgccgctcc
     8881 ccaggctttg cgatacacaa tctgattatc tttaatgatc agcaggttta cgccgggata
     8941 accggcatca acttgctggc taatccagcg atccatctgg ttaagccgtt tgacgttaaa
     9001 ccctgctttc tctggcgagc tttctgtcag aacagggtat tttgcggcgc tgacgctaca
     9061 actaacagcc agcagagaaa gatagagcat tgtccgtttc atgatatcag tccagattga
     9121 cgttacggca gccaaagagc gtggtgaaaa taaacccgca aacccatgcc accagaatcc
     9181 cgccagcata aaccgccatg gccggaagga tgccttgtgc ggaagtcatc agcggcagcg
     9241 ctaccagacc agacggccca aaggcgctgt ttaagcccat cggcagaccc caccaggcta
     9301 tcaggccgat aaacaaaccg cccgccgcac cgcctaaaca ggcggtgaca aacggcttca
     9361 tgcggggcag ggtgacaccg taaatcagcg gttcaccaac gcccagcagg ccgggaataa
     9421 tcgccccgcg tacctgactg cgtagcgcac tgtgcggttg cgaccgccag tagagtgcca
     9481 gcgccgcgcc cacctggccc gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc
     9541 cctggctgtc cattaacgcg aggtaaacag gaataaagcc ctgatgcacg ccaaatacca
     9601 cggcgatcag gaacagcccg gctaaaaccg cacaaccgaa cggattactg ttcaggtgca
     9661 taaacagcca tgacatacct tcgaataacc agccacccag cggcataatg atcaggtacg
     9721 caagcgtagc ggtgatcagc agggtgatta acgaggtcag cagcatgtcg agatcgtccg
     9781 gcataaagcg gcgcaccatg ccttcaatgc gggcgcaggc ccacgcggca atcagcacgc
     9841 cgataatatt gccgcgcgga tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt
     9901 aaccggtggt tgcagcgggg ttataaccga gcaaaaagag tgcggcaata attgcgccat
     9961 tgacgcccgt gccgccaaat gcctgggcgg cgttatagcc caccagaatc accaggaagg
    10021 tgaacaaacc tttgctgaac accttcataa aattcagcgc gtcgggtagc gtgccctgag
    10081 catctgccgg aacgtgcatc accgtggcaa ttaacgtcgc tatccccagc agcagaccgg
    10141 cggcaataaa accggggatc agcggcgtga agatggtggc gaatttggcg agaaattgtt
    10201 gcacgccgga ggtttgtttg gcttttaact gacgtttgtt ctgggcggcg atttctgcgg
    10261 catcctgtac cggtgcctcg cccagcagct cgctcatcgc ttttgcggca cgatgcgctt
    10321 taccaggtcc aaaaacgacc tgcacctggt cgctggtcaa aatgacgctc ttcacgcctt
    10381 ccagcgtttt gatattggga tcaaccagtg aactgtcatg tacacccaga cgcaggcgcg
    10441 tcatacagtt accacaactg gcgatatttc ccggtccgcc gacacgggta agaatggtgt
    10501 tcagaagttc actgctgatc tctttggcca tgggttattc cttgtctaaa acctgacgaa
    10561 taaagccgcc gtgttgatcc aggcgttttt tagcttctgc ggcatcgaga tttttcagca
    10621 ccatcacaat ggccgtttta cagttgcgct cgcaggcaat taacgccgct tccgcttgtt
    10681 cggcattaca tccggtggcg tttttaacga tattgacctg gcgcacatgc agtttttcgt
    10741 tggtggcgac cacatcgacc ataaggttgc cgaacacttt gccggattta atcatcagcc
    10801 cggtggaaag catattgagc accagtttct gcgctgtacc tgctttcatc cgcgaagaac
    10861 cggtaacaac ttcggcacct acaatcggtg taatggcaaa ctcagcggtg gttgaaacgg
    10921 cgctccccgg attacaggaa attcccactg tgcggcagcc gagctggcgt gcgtattcca
    10981 gtccggcaat cacatacggc gtgcgaccgc tggcagcaat gccaaccacc acatcctgtg
    11041 ccgttaaatt aatatttttc agatcattaa caccgccttc ccggctatct tccgcgcctt
    11101 ccaccgcgtg ctgaatggca tattcgccgc cagcaatcaa accaaccacc agacccggtt
    11161 tcacgccgta ggtgggcgga cattcgctgg catccagaat ccccagacga ccggatgttc
    11221 ccgcaccgag gtaaatcaga cgcccgccgc cgcttacctg ggcgtggata acatcgatcg
    11281 ccgcggcgat atccggcagt acgcgctcaa cggcaagcgg tacggtttta tcttcatcgt
    11341 tgataatccg gcacatttcc agcgttgata cgcggtcaat ttcagccgag gcggcgttcg
    11401 agccttcagt aatcatcttt tcaagttgca tcggtggtga ccttactatt tacgatcaaa
    11461 taattgcaga tgtatggaat catatatcct gaattgacag acttaaagga atcattgagt
    11521 ctacactcgc agagtgtgaa gagcgtcata agaacttaag cggaatttat gctgccagcc
    11581 caggagagtg gaagatgttg tacctgacga aaataagcaa tgcggggagt gaattcaccg
    11641 aaaacgagca aaaaatcgct gactttctgc aggccaacgt gagtgagctg caatccgttt
    11701 cttcacggca gatggcgaaa cagctcggca ttagccagtc gagcatagtg aaatttgccc
    11761 aaaaactcgg cgcgcaagga tttactgaat tgcgtatggc gttaataggc gaatacagtg
    11821 ccagccggga gaaaacgaat accacggcgc tgcacctgca cagttcgatt accagtgatg
    11881 attcgctgga agtgattgcc cgcaaattga atcgcgaaaa agagctggcg ctggagcaaa
    11941 cctgtgcgtt gttcgactac gcgcggctgc aaaaaatcat tgaggtcatc agtaaagcac
    12001 cgtttatcca gataacgggc ctgggcggat cggcgctggt ggggcgcgat ctgtcgttca
    12061 aactgatgaa aattggctat cgcgtggcct gcgaagccga cacgcacgta caagccaccg
    12121 tttcccaggc gttgaaaaaa ggcgatgtac aaatcgctat ctcttacagc ggtagtaaaa
    12181 aagagattgt cttgtgcgta gaagcggcac gaaagcaggg ggcaacggtg attgccatta
    12241 cttctctggc tgactctccg ctgcggcggc tggcgcattt cacgctcgat accgtttccg
    12301 gtgaaaccga atggcgtagc tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg
    12361 atttactgtt tgttggtctt gtgcaactca atgatgtggc gtcattgaaa atgattcagc
    12421 gcagcagtga gctaacacaa cgcctgaaat gatgcataaa gcagcgactg gattgagatt
    12481 ttcctgaatt agtgagctga tccgcagcaa tattttgttt atcctgtatt ttcagaggga
    12541 atggagtgta acgctctgta ttaacaagga gagcattaaa atgggtaaac tcacgggcaa
    12601 gacagcactg attacgggcg cattgcaggg aattggcgaa ggaattgcca gaacttttgc
    12661 acgtcatggc gcgaacctaa tcttgctgga tatctcccct gagatcgaaa agctggcgga
    12721 cgaactgtgt ggtcgtggtc atcgctgtac ggcggttgtc gccgatgtgc gtgacccggc
    12781 gtcggtagcc gcagctatca aacgcgcgaa ggaaaaagaa gggcgcattg atatcctggt
    12841 gaataacgca ggcgtttgtc gtctgggcag tttcctcgat atgagcgatg acgatcgcga
    12901 tttccatatt gatatcaata ttaaaggcgt atggaacgtc acgaaggcgg tgctgccgga
    12961 gatgattgcg cgcaaagatg gtcgcattgt gatgatgtct tcagtcactg gtgatatggt
    13021 ggccgatcct ggcgaaacgg cgtacgcctt aacgaaagcg gcgattgttg gcctgacaaa
    13081 atcgctggcg gtggagtacg cgcagtctgg tattcgcgtt aacgccattt gcccgggata
    13141 cgtgcgcaca ccaatggcgg aaagcattgc ccgccagtcg aacccggaag atccagagtc
    13201 ggtgctgact gaaatggcga aagcaatccc gatgcgtcgc ctcgccgatc cgctggaagt
    13261 cggcgaactg gcggccttcc tcgcatcgga tgaatccagc tatttaaccg gtacacagaa
    13321 tgtgattgat ggcggcagca cactgccgga gacggttagc gtcggtatct gattcacctc
    13381 tgtttcctcc ctgcatgtgt ggggaggatt tcgtcttgaa ctaagttcac caggctattt
    13441 tatttgtcat tttggccccg ggcagtgctc gaaatactca cgtactatgt gtacgctccg
    13501 gtttctccgc gctgtccgtg tccaaactgc ctgcaacaat tacgcctgtt gaaccaagtt
    13561 cttattccct ttttaacttc caaatcacca aacggtatat aaaaccgtta ctcctttcac
    13621 gtccgttata aatatgatgg ctattagaaa gtcattaaat ttataagggt gcgcaatggc
    13681 cgttaactta ctgaaaaaga actcactcgc gctggtcgct tctctgctgc tggcgggcca
    13741 tgtacaggca acggaactgc tgaacagttc ttatgacgtc tcccgcgagc tgtttgccgc
    13801 cctgaatccg ccgtttgaac agcaatgggc aaaagataac ggcggcgaca aactgacgat
    13861 aaaacaatct catgccgggt catcaaaaca ggcgctggca attttgcagg gcttaaaaac
    13921 cgacgttgtc acttataacc aggtgaccga cgtacaaatt ctgcacgaca aaggcaagct
    13981 gatcccggct gactggcagt cgcgcctgcc gaataatagc tcgccgttct actccaccat
    14041 gggcttcctg gtgcgtaagg gcaacccgaa gaatatccac gactggaacg acctggtgcg
    14101 ctccgacgtg aagctgattt tcccgaaccc gaaaacgtcg ggtaacgcgc gttataccta
    14161 tctggcggca tggggcgcag cggataaagc tgacggtggc gacaaagcca aaaccgaaca
    14221 gtttatgacc cagttcctga aaaacgttga agtgttcgat actggcggtc gtggcgcgac
    14281 caccactttt gccgagcgcg gcctgggcga tgtactgatc agcttcgagt cggaagtgaa
    14341 caacatccgt aaacagtatg aagcgcaagg ctttgaagtg gtgattccga aaaccaacat
    14401 tctggcggaa ttcccggtgg cgtgggtcga taaaaacgtg caggccaacg gtacggaaaa
    14461 agcagcaaaa gcctacctga actggctcta cagcccgcag gcgcagacca tcatcaccga
    14521 ctattactac cgcgtaaata acccggaagt catggacaaa ctgaaagata aattcccgca
    14581 gaccgagctg ttccgcgtgg aagacaaatt tggctcctgg ccggaagtga tgaaaaccca
    14641 cttcaccagc ggcggcgagt tagacaagct gttagcggcg gggcgtaact gatgtttgca
    14701 gtttcatcaa gacgcgtgct gccgggcttt accttaagcc tcggcaccag tctgctgttt
    14761 gtgtgcctga ttttgctgct gccgctctcc gcgctggtga tgcagctggc tcagatgagc
    14821 tgggcgcagt actgggaggt gatcaccaac ccacaagtgg tcgcggccta caaagtgacg
    14881 ctgctgtcgg cgtttgtggc atcgattttt aacggcgttt tcggtctgct gatggcgtgg
    14941 attttaacac gttatcgctt tccgggccgc acgctgcttg atgcgctgat ggatttaccc
    15001 tttgcgctgc caacggcagt cgccggttta acgctggcct cgctcttttc cgtaaacggt
    15061 ttttacggtg aatggctggc gaagtttgat atcaaagtca cctatacctg gcttggtatt
    15121 gcggtggcga tggcctttac cagcattccg tttgtggtgc gtaccgtgca gccggtgctg
    15181 gaagagttag gcccagaata tgaagaagcg gcggaaacgc ttggtgcaac gcgctggcag
    15241 agtttctgca aagtggtgct gccggaactt tctccagcgc tggtggcggg cgtggcgttg
    15301 tcgtttaccc gtagtcttgg tgagtttggc gcggtgatat ttatcgccgg gaacatcgct
    15361 tggaagacgg aagtgacgtc gctgatgatt tttgtgcgct tgcaggagtt tgattacccg
    15421 gcggcgagcg cgattgcttc ggtgatcctc gcggcatccc tgctgctgct gttctcaatt
    15481 aacactctgc aaagtcgctt tggtcggcgt gtggtaggtc attaatggcg gaagttaccc
    15541 aattgaagcg ttatgacgcg cgcccgatta actggggcaa atggtttctg attggcatcg
    15601 ggatgctggt atcggcgttc atcctgctgg tgccgatgat ttatatcttc gtacaggcgt
    15661 tcagcaaagg gctgatgccg gttttacaga atctggccga tccggacatg ctgcacgcca
    15721 tttggctgac ggtgatgatt gcgctgattg ccgtaccggt gaacctggtg ttcggcattc
    15781 tgctggcgtg gctggtaacg cgctttaact tccccggacg ccagttactg ctgacgctac
    15841 tggacattcc gtttgccgta tcgccggtgg ttgcaggtct ggtgtatctg ctgttctacg
    15901 gctctaacgg tccgctgggc ggctggctgg atgaacataa tttgcagatt atgttctcct
    15961 ggccgggaat ggtgctggtc accatctttg tgacctgtcc ttttgtggtg cgcgagctgg
    16021 tgccggtgat gttaagccag ggcagccagg aagacgaagc ggcgattttg cttggcgcgt
    16081 ctggctggca gatgttccgt cgcgtcacat tgccgaacat tcgctgggcg ctgctgtatg
    16141 gtgtggtgct aaccaacgcc cgcgcgattg gcgagtttgg tgcggtgtcg gtggtttccg
    16201 gctcgattcg cggcgaaaca ctttcgctgc cgttacagat tgaattgctg gagcaggact
    16261 acaacaccgt cggctccttt accgccgccg cgctgttgac gctgatggcg attatcaccc
    16321 tgtttttaaa gagtatgttg cagtggcgtc tggagaatca ggaaaaacgc gcgcagcagg
    16381 aggagcatca tgagcattga gattgccaat attaagaagt cgtttggtcg cacccaggtg
    16441 ctgaacgata tctcactgga tattccttct ggtcagatgg tcgcgctgct ggggccgtcc
    16501 ggttccggaa aaaccacgct gctgcgcatt attgccgggc tggagcatca aaccagcggg
    16561 catattcgct tccacggtac cgacgtgagc cgcctgcacg cacgcgatcg taaagtcggt
    16621 ttcgtgttcc agcattacgc gctgttccgt catatgacgg tgttcgacaa tatcgctttt
    16681 ggcctgacgg tgctgccacg tcgcgagcgc ccgaatgccg cagccatcaa agcgaaagtg
    16741 acaaaattgc tggagatggt ccagcttgcg catctggcgg atcgttatcc ggcacagctt
    16801 tccggcggcc agaaacagcg cgtggcgctg gcgcgcgcgc tggctgttga accgcaaatt
    16861 ctcctgcttg atgaaccgtt tggcgcgctg gatgcgcagg tgcgtaaaga gctgcgtcgc
    16921 tggctgcgtc aactgcatga agagctgaaa ttcaccagcg tgttcgtgac ccacgaccag
    16981 gaagaagcga ccgaagtagc tgatcgtgta gtggtgatga gccagggcaa tatcgagcag
    17041 gctgatgcgc cggaccaggt atggcgcgaa ccagcgaccc gatttgtgct cgaatttatg
    17101 ggcgaagtga accgcctgca gggaaccatt cgcggcgggc agttccatgt tggcgcacat
    17161 cgctggccgc tggggtatac gcctgcgtat caggggccgg tggatctctt cctgcgcccg
    17221 tgggaagtgg atatcagccg ccgtaccagt ctcgattcgc cgctgccggt acaggtactg
    17281 gaagccagcc cgaaaggtca ctacacccaa ttagtggtgc agccgctggg gtggtacaac
    17341 gaaccgctga cggtcgtgat gcatggcgac gatgccccgc agcgtggcga gcgtttattc
    17401 gttggtctgc aacatgcgcg gctgtataac ggcgacgagc gtatcgaaac ccgagatgag
    17461 gaacttgctc tcgcacaaag cgcctgatag gttgagtgaa tgttaaacgc ccggaggcgc
    17521 ttcccgcgat ccgggctttt taatggcaag gtttgtaacc tgtagacctg ataagacgcg
    17581 caagcgtcgc atcaggcaac accacgtatg gatagagatc gtgagtacat tagaacaaac
    17641 aataggcaat acgcctctgg taaagttgca gcgaatgggg ccgaataacg gcagtgaagt
    17701 ctggttgaaa ctggaaggca ataacccggc aggttcggtg aaagatcgtg cggcactttc
    17761 gatgatcgtc gaggcggaaa agcgcgggga aattaaaccg ggtgatgtgt taatcgaagc
    17821 caccagtggt aacaccggca ttgcgctggc aatgattgcc gcgcttaaag gctatcgcat
    17881 gaaattgctg atgcccgaca acatgagcca ggaacgccgt gcggcgatgc gtgcttatgg
    17941 tgcggaactg attcttgtca ccaaagagca gggcatggaa ggtgcgcgcg atctggcgct
    18001 ggagatggcg aatcgtggcg aaggaaagct gctcgatcag ttcaataatc ccgataaccc
    18061 ttacgcccat tacaccacta ccgggccgga aatctggcag caaaccggcg ggcgcattac
    18121 ccattttgtc tccagcatgg gaacaaccgg cactatcacc ggcgtttcac gctttatgcg
    18181 cgaacaatcc aaaccggtga ccattgtcgg cctgcagccg gaagaaggca gcagtattcc
    18241 cggcattcgc cgctggcctg cggaatatct gccggggatt ttcaacgctt ctctggtgga
    18301 tgaggtgctg gatattcatc agcgcgatgc ggaaaacacc atgcgcgaac tggcggtgcg
    18361 ggaaggaata ttctgtggcg tcagctcagg cggcgcggtt gctggagcac tgcgggtggc
    18421 aaaagctaac cctggtgcgg tggtggtagc gatcatctgc gatcgtggcg atcgctacct
    18481 ctccaccgga gtgtttgggg aagagcattt tagccagggg gcggggattt aagattattg
    18541 cgcaggcacc gttttatccg tctgcgcaat tagcgcatgt aaaaaaggct gtgcgttctc
    18601 atcgctctta ccggggatct tcacaatata cggcttcccg gtaatcgatg acgacgaagc
    18661 aaccttatca ataaactgct cggcagtgtc aatgcggtta cgggtattgc cgagcttcag
    18721 acgcagatga gaaaccgctt catcgcaggt atgttcatca ccgttgcgca caaatatcaa
    18781 atcctttttc tgtgataatc cctccagcat ggcgttgatg cgggcttctt catgggcagt
    18841 taacttcgcg taagcgggga gtgtcattag tagtgtaatg accagacaaa tgattttctt
    18901 cattgaatta ccttatcctt gttttgtaat agccgatttg actgccagaa tcaggtaaag
    18961 tttcggttgg aatacgatga ggatcatcgg caaggagaaa aaattaaatg agtagtttgt
    19021 tgttgtttaa cgataagagc agggcgctgc aggcggatat cgtcgccgtg cagtcgcagg
    19081 tggtttacgg cagcgtgggc aacagcattg ccgtgcctgc tatcaaacag aacggtctga
    19141 atgtttttgc cgtgccgacg gtattgctga gcaatacgcc gcattatgac actttctacg
    19201 gtggtgcgat tccggatgaa tggtttagcg gttatttgcg tgcgcttcag gagcgtgatg
    19261 cgctgcgcca gctgcgcgct gtaaccacgg gctatatggg aacggcatcg caaatcaaaa
    19321 tccttgccga gtggctgact gcgctacgca aagaccatcc tgacctattg atcatggtcg
    19381 atccggtgat tggcgatatt gatagcggaa tttatgtcaa acctgacctt cccgaggcgt
    19441 atcgacaata tttactgccg ctggcgcagg gaattacccc caatatcttt gagctggaaa
    19501 tcctgaccgg taaaaattgc cgcgatctcg acagtgccat tgctgccgca aaaagtctgc
    19561 tttcagacac attaaaatgg gtggtgatta ccagcgcctc cggtaatgaa gaaaatcagg
    19621 agatgcaggt ggtggtggtc agtgccgaca gcgtgaatgt catttcccat tcacgggtaa
    19681 aaaccgacct gaaagggact ggcgacctgt tttgtgctca gcttatcagt ggtttgctga
    19741 aagggaaggc gttaaacgat gcggtgcacc gtgcggggtt gcgcgtactg gaagtgatgc
    19801 gctacaccca gcagcatgag agcgatgaat tgattttgcc gccgctggcg gaagcataaa
    19861 aaatggcgcc gatgg
//
LOCUS       DAJTOR010000067        19828 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTOR010000067 DAJTOR010000000
VERSION     DAJTOR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.067"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(49..1071)
                     /locus_tag="OO249_002093"
                     /pseudo
     CDS             complement(49..1071)
                     /locus_tag="OO249_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(1081..1893)
                     /gene="hcaB"
                     /locus_tag="OO249_002094"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(1081..1893)
                     /gene="hcaB"
                     /locus_tag="OO249_002094"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5636392.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(1890..2210)
                     /locus_tag="OO249_002095"
     CDS             complement(1890..2210)
                     /locus_tag="OO249_002095"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5636393.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGSDIFIDLP
                     EAQP"
     gene            complement(2210..2728)
                     /locus_tag="OO249_002096"
     CDS             complement(2210..2728)
                     /locus_tag="OO249_002096"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5636394.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRPRHLISNC
                     QISETDIPNVFAVRVNYLLYQAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(2725..4086)
                     /locus_tag="OO249_002097"
     CDS             complement(2725..4086)
                     /locus_tag="OO249_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5636395.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKSPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            4222..5112
                     /gene="hcaR"
                     /locus_tag="OO249_002098"
     CDS             4222..5112
                     /gene="hcaR"
                     /locus_tag="OO249_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5636396.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKTVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            5272..6411
                     /locus_tag="OO249_002099"
     CDS             5272..6411
                     /locus_tag="OO249_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5636397.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(6403..7683)
                     /gene="csiE"
                     /locus_tag="OO249_002100"
     CDS             complement(6403..7683)
                     /gene="csiE"
                     /locus_tag="OO249_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5636398.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(7874..8728)
                     /locus_tag="OO249_002101"
     CDS             complement(7874..8728)
                     /locus_tag="OO249_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5636399.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(8873..9676)
                     /gene="suhB"
                     /locus_tag="OO249_002102"
     CDS             complement(8873..9676)
                     /gene="suhB"
                     /locus_tag="OO249_002102"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5636400.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAVEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            9795..10535
                     /gene="trmJ"
                     /locus_tag="OO249_002103"
     CDS             9795..10535
                     /gene="trmJ"
                     /locus_tag="OO249_002103"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5636401.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10805..11293
                     /gene="iscR"
                     /locus_tag="OO249_002104"
     CDS             10805..11293
                     /gene="iscR"
                     /locus_tag="OO249_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5636402.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            11405..12619
                     /gene="iscS"
                     /locus_tag="OO249_002105"
     CDS             11405..12619
                     /gene="iscS"
                     /locus_tag="OO249_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5636403.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            12647..13033
                     /gene="iscU"
                     /locus_tag="OO249_002106"
     CDS             12647..13033
                     /gene="iscU"
                     /locus_tag="OO249_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5636404.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            13050..13373
                     /gene="iscA"
                     /locus_tag="OO249_002107"
     CDS             13050..13373
                     /gene="iscA"
                     /locus_tag="OO249_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5636405.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            13469..13984
                     /gene="hscB"
                     /locus_tag="OO249_002108"
     CDS             13469..13984
                     /gene="hscB"
                     /locus_tag="OO249_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5636406.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            14001..15851
                     /gene="hscA"
                     /locus_tag="OO249_002109"
     CDS             14001..15851
                     /gene="hscA"
                     /locus_tag="OO249_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5636407.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            15853..16188
                     /gene="fdx"
                     /locus_tag="OO249_002110"
     CDS             15853..16188
                     /gene="fdx"
                     /locus_tag="OO249_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5636408.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            16200..16400
                     /gene="iscX"
                     /locus_tag="OO249_002111"
     CDS             16200..16400
                     /gene="iscX"
                     /locus_tag="OO249_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5636409.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            16578..17861
                     /gene="pepB"
                     /locus_tag="OO249_002112"
     CDS             16578..17861
                     /gene="pepB"
                     /locus_tag="OO249_002112"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5636410.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            18003..18779
                     /gene="sseB"
                     /locus_tag="OO249_002113"
     CDS             18003..18779
                     /gene="sseB"
                     /locus_tag="OO249_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5636411.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(19272..>19828)
                     /gene="sseA"
                     /locus_tag="OO249_002114"
     CDS             complement(19272..>19828)
                     /gene="sseA"
                     /locus_tag="OO249_002114"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR5636412.1"
                     /translation="LFSAPRAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELP
                     EGEFNAAFNPEAVVKVTDVLLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGA
                     LNVPWTELVREGELKTTDELDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVP
                     NVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT         4719 a         5058 c         5402 g         4649 t
ORIGIN      
        1 tgccattcta tttcaggaag gagtgtccgt taaactcagg ctacctcagt catcgccacg
       61 catatcgcca ataaactgta agttatcact gtactgatcg ctccagaacc acggcagtag
      121 cggtagcggt agccctaaca ttgcggcagc ggcaatttgc gcctggttat tggcgttttc
      181 ccagctttca cagcggtgta gtgcaccatt atcaagacga gtgattgcca catcgccacc
      241 ggcaaagatc gcgggatcgc aggtgcggca agcctcatca atgacaatgc cattggcagt
      301 atcaaggttg gcctcgcgag ccagttgctc gttggcgctg ataccaatac cgtaaatcac
      361 cacatcagcc tgaagcgttt ccccactttg cagcgtcagt tctacttttt caccatcgac
      421 cacatgttca atggcattat tgagcagaat gcgcacacca gcctgctggt ggcgctgtaa
      481 aagatagcgt tgcacgggcg gtggtgcatt acggcccatg acggttgccg ccagttcaat
      541 cactgtcacc ttacatctgc gctgcgctgc gctgcgctgc gtggcgctgg cagccagttc
      601 cagaccaata gttccggcac cgacaatcac gactgaccgt tcgggctgca gaacttctcg
      661 cagtctggcg gcatcaccgg catggcgtag ggtaaagcag cgttctccca gtgcatcaag
      721 caacggcagc ggtcgagctg ccgcgccggt tgctataaaa agctgatccc agtgccagct
      781 ttcgccgttg gttaacacta actctcgtgt gtcgcgaccc agcgttttga tggttacacc
      841 ggaatgcaga tgaacattgt tttcctgcca ccagttagcg ggtaacacct gctgtaactg
      901 cggggaatct tccagcaaca tggatttcga gagtggaggt cgttcataag gaagatgtcg
      961 ctcatcggaa aacagatgca gctcaccggt gaacccttgc tggcgtagcg aggccgcagc
     1021 cattgccgcc gcttgcacgc caccgacaat aatgatcgtt ttttctttca tcgtgcttcc
     1081 ttaaagatcc agcccagccg ctacgtggcg aatgccgcga atcgctaaac ccgcatcagc
     1141 gttgatcatc acaccgctta atgcgcgatt attgcgccgc gatgccaaca tcacatacgg
     1201 ccccgtaaaa tccgccggtt gcgggaaaaa ttgcagcggt aaaatggcgg caattttctc
     1261 cggcgtcaga gactgcatta tcgaggtttc actttgcccg agcgcctgtg ggccgcgcag
     1321 gtcgctggcc ataccacacg ggccgacgcc attcacccgc actttcggtg ccagttcata
     1381 agccagttgg cgaataagtc cggttgcggc atgtttactg gcggtgtaca gcgggccacc
     1441 gccgccagga taccaggcgg catttgacag tgtgaaaatc atgctgcctt cactggcgat
     1501 taacgccgga gcgcaggctt ttgcgcccag caggtaaccg agtacgttga cgttaaacag
     1561 ctcgtggaag ccggtttcga gcgtctctgc gggagtatta accagtgagg cattgtgatc
     1621 ccagatgcct gcattgccga taaaacaatc cagcttgccg gaacgagtca ggatctgatc
     1681 gaccgcgcgt tgataatcgg cataacaggt cacgttacct tccaccgcca gaatatgttc
     1741 gccaaatcgc tgacgcagac tggcgacttt tgccgccgac agttccagcg tggcaacctg
     1801 cgcgccttct tcgataaatc gctcgaccag cgccagccct aatcccgatc cgccgccggt
     1861 aataaaaatg gactcgttat gcagatcgct catggctgcg cctccggtaa gtcgatgaaa
     1921 atgtcactac cttcaacgtg tactggataa gtggtgagcg gatcggtggc gggcaggcat
     1981 aacgctttcc ccgttttcag gcaaaaactg gcggcgtgta gcgggcactc caccgtggcg
     2041 tcatcttcca gatacccttc tgacatcgac gcattaccat ggctgcaacg atcgttaatg
     2101 gcataaaact cgccgccaac gttgaacagg gcgatgacgg gcgaggtatc gatccggaga
     2161 gcctcaccct ccggcacatc cgcaacggga cacgcataaa ttcgattcat cagaacagta
     2221 cactcaggtt atgggaagtg attaccgctt gatccaggac gatatcccgt tccagcaagc
     2281 gccagttgtc atcttccaga cggcgaactt tgtcgaaacg cgttccaaca tagaatgttt
     2341 catcgcgctc tttttgtgcc tgataaagca gataatttac ccgcacagca aatacgtttg
     2401 ggatgtcggt ttcgcttatc tggcagttgc tgattaagtg acgggggcgt gacggcggct
     2461 cttctgccca ggccatgccc gtttccagac gggcgattcg ccgctccagc tggtctttgg
     2521 tgtcattaaa aatccaggtt gtcggtggct ggacgccttt gcggcggtcg cgtgtttgcg
     2581 cgttaactgt ggtgcgcatg gtgtaacgaa tctcttcgtc gagctgcgcc agccagtcac
     2641 gaaatttcca gtcgtccagt aagctggctt cgtgaaagag aaactggcta atgcggagat
     2701 gtaactctag tgaaacttgc gcactcattt catcacctcc tgctggtaag cggcggtttt
     2761 atcgagcact tcctgccagc tttcgctact cagaagatcg gcccagcgtt ggtacattcc
     2821 acgagcggca gtttctgaaa agatatagtt agtaatgcca ggaatgccgt cgtcgcggcg
     2881 cttttcctga ccaagcccca tttccagaca cagtttgctg ttgcgggcgc ggtggccttt
     2941 aagcaatttc tggatttcac accagttctc cgagtcatcc tgctcgagaa aaccagcagg
     3001 accaaaagca cgagtggcgc tgttttcaaa agcggcttta acttcatcgg aggcggcttt
     3061 gtcagtaata cagaacgccc acacttcaac ttgatcaggg ccgcgcggat gccagacgcg
     3121 gagtgtggca gtgccgttta gccatgaaag cgtggggaat atattgttat gacccgccag
     3181 gcgcagggcg cgaacttcac ctaaacgttg ttctgcttcg gcataggttt cgcggtaata
     3241 gctggaaacc gcgccatcga cccagacatt agcatccggt ttttcagtaa agaagaaacc
     3301 gctaccgtga ccgtcctgac caaattgcag cgcatctttg gcggtttccc acaccgggcg
     3361 ggcggtttgt ccatcaccga gacgcttatc gctgccatca tctttcgccc ctaatacctg
     3421 aacggcagaa gcatggctga acagagcatg atattggtca ctggcgaact gctctgccgg
     3481 ggatttccag ttacagttga tcacccactt ttgtacgccg ccgacaattt cggtgccgcc
     3541 ttcgcgacga tccagcatgc catccagata ccaggcaatg tcaccaaggt aatcacgcag
     3601 gcccggtgcg ctggtatccc agttgccaaa aatcagccct ttataactct ccacacaagg
     3661 aacttcgttt agcccccagt gggatttaca caacccttgt gggtaggcgc gaggttccag
     3721 cggtacatcg atcaactcgc cgttaatgcc ataagaccag ccgtgatacg ggcaggtaaa
     3781 ggcgcgagtg ttgccgcaat ctgcataact cacacgcatg gcccggtggc ggcattggtt
     3841 gagaaacgcc ttgatgctgc cgtctttctg acgcactacg acaaccgcat cttctcccat
     3901 gtaggtgtta aagaaatcac cgggttttgg gatctggctt tcgtgggcga gaaatagcca
     3961 gcaacgaccg aaaatacgct caagctccag ttggtaaata tccgggtcgg tataaatacg
     4021 cggagtgacc cgaccatttt gggtgtcaat cagttggtaa atgttcaaat ctgagggtgt
     4081 ggtcatattt tttaatcctg aaagataaga tgtattgacc ttctattttt gtgccataag
     4141 gatgttgctg gttggttgct aatatgtgaa atagttaatt tctaccagct gagacgtttt
     4201 ttcgataaca aggggaaggt gatggaacta cggcatttac gctatttcgt cgcagtggcg
     4261 caggcactga actttacccg tgcggcggaa aaactgcata cctcacagcc ttcgttaagc
     4321 agccagatcc gcgatcttga aaactgtgtc ggtgttccgt tactggtgag ggataagcgc
     4381 aaagtcgcgt tgacggcggc gggagaatgt tttctccagg acgcgctggc aatcctcgaa
     4441 caagcggaaa atgccaaatt acgggcgcgg aaaactgttc aggaagacag acaattaacc
     4501 attggctttg tgccatcggc ggaagtgaat ttactgccaa aagtattacc gatgtttcgt
     4561 ctcagacagc cagacacctt gattgagctg gtgagtttaa tcaccacgca acaggaggaa
     4621 aaaattcgcc gtggtgaact cgacgtcggc ttaatgcgcc atcctgttta tagcccggag
     4681 attgattatc tggagctttt tgacgaacca ttagtggttg tgttaccggt tgatcaccct
     4741 ttagcacatg aaaaagagat caccgccgcg caactggatg gcgtgaattt cgtcagtacc
     4801 gatccggcgt attccggttc gcttgcgccg atcgttaaag cgtggtttgc gcaagaaaac
     4861 agccagccaa atatcgtcca ggtggcaacg aatattctgg tgaccatgaa tctggtgggc
     4921 atggggctgg gcgtcacttt gatacccggt tatatgaata attttaatac cggacaggtt
     4981 gtttttcgtc ccattgccgg aaatgtgcct tccattgctt tactgatggc gtggaagaaa
     5041 ggggagatga agccggcatt acgcgatttc atcgccattg tgcaggaacg tttggcaagc
     5101 gtaacggcat aattacgcat tatttttgca acatgcgtcg cggatcgcag ttttttagtg
     5161 cttgtgaact ggcgttacag acatcgggaa gcaggctcga atattcttat ttttcaactt
     5221 gcaccaacat tcgattaacc ccagactaac gcctcctgac gggagggact catggttttg
     5281 caatccacgc gctggttggc gctcggctat ttcacatact tttttagtta cggcattttt
     5341 ctacctttct ggagcgtctg gcttaaaggg attggtttaa cgccagaaac catcggcctg
     5401 ttattggggg caggtctggt tgcccgtttt ctcgggagtt tgctcatcgc gccccgcgtc
     5461 agcgatcctt ctcgcctgat ttccgccttg cgcgtgctgg caatgatgac acttctcttt
     5521 gctgtcgcct tctgggcggg ggcgcacgta gcgtggctga tgctggtgat gattggcttt
     5581 aacctctttt tctcaccgct ggtaccgttg accgatgcac tggcgaatac gtggcaaaag
     5641 cagttcccgc ttgattacgg caaagtgcga ctgtggggct cggtggcgtt tgtcattggc
     5701 tcggcgctga cgggcaaact ggtcactatg tttgattatc aggtgatcct cgcgctgtta
     5761 acattgggcg tggcatccat gctgctcggc tttctcatcc gtccgacgat tcagccacaa
     5821 ggggcaagcc gccagcagga gagcaccggt tggtcagcgt ggttggcgct ggttcttcag
     5881 aactggcgct ttctggcctg cgtttgttta ttgcaggggg cacatgcggc ctattacggt
     5941 tttagcgcca tttactggca ggcagctggc tactcggcct cggcggtggg gtatttgtgg
     6001 tcgctgggcg tggtggcgga agtcattatc tttgcgctga gtaataaact tttccgccgt
     6061 tgtagtgcac gcgatatgct gttgatctcg gcgatttgcg gcgtagtgcg ctggggcatt
     6121 atgggagcaa ctacggcgtt gccgtggttg atagtggtgc aaattctgca ttgcggcacc
     6181 ttcacggtct gccacctggc cgccatgcgc tatattgccg ctcgccaggg tagcgaagtc
     6241 atccgtttac aggcggttta ctctgccgtc gcgatgggcg gcagtatagc tatcatgacc
     6301 gttttcgccg gtttcctgta tcaatatctg ggccacggcg tgttctgggt aatggcgctg
     6361 gtggcgcttc cggcaatgtt tttgcgcccg aaagttgttc cctcatgctg attcgagcat
     6421 tttgcggatc tgctcctgtt gatgtgttgt cagtgtcagg tccgtataga tcagcggtgg
     6481 tgaaaagagc ggtaacggca tggtataagg cgcaataatc agtgccgcgc cgcgcggagc
     6541 gcctgtctgc aaaaatgcct ttaccgacat atgtttaatg ttgagcggca gtaacgttag
     6601 ttcgcgtaac tgctgctcaa tctgcgcttc tcgctcgcta tcattcccgg taagaagaat
     6661 aatctgtttt tcatgcagat cgttttcctg cattagccag gcaccgaaaa tcaccgcgac
     6721 cagaccactt tcctcatcag aaagatggac gccgtattca ctttcaaatc cggccagcgc
     6781 cgcgcgggtg gtgcgcacca ggcgtgggta cagtctggcg aactcttctg gcagggtatt
     6841 atcaataccg atggcaaaca aactgcgatt taacgcctgg gcgaggtggg tataaagctg
     6901 atcgcataac ccctgttcat cataaaaacg aacatttccc agctcacgaa aatggtttac
     6961 cagacgtttg atagactgac gcaattgtct gtctcgctga tgcgcatcac gcaatggatc
     7021 gggaacccgc aacatggaaa aaagtaatgc cataaacagt ggctcatcag gcggtacagg
     7081 ctggagcgcc cgacgctgcc agtggcgtcc aatttcctgc gctacctgaa attcaaggca
     7141 ggattccgcc cagcgacgtt ggagcggatt aaactgaggc gtaattccag cgtggtgttg
     7201 cagcaaacaa tattgcagat acagacacag gaaatgaata tcgcgcgttt caaagcgttt
     7261 ttgcagccgt cgggagcaga gattcactaa cgcttgtaga ttggtatcgt cgtaaaagtt
     7321 acgcgcaatt ccgcgccgct tcagttcact cttcagggcg ggggtgaatt ggctggtaat
     7381 aaatgacgga cacagacgga aaccacgtcg tagccagtga aataaacaca aacgttggtt
     7441 aagcactgta ccttcaaccc ggtagctacc gtcataacca gtcgtcagtg tgagttgatg
     7501 atagcgcagg atctcccgtc ctgtttcgct gatatcaaga ctggcaatat catcatccac
     7561 accattaagc tcgctgaagg ttgccatggt ggcggtcaac cccggctgaa agagcgtcag
     7621 caagatctgg cagcggcgct ggggagccga aaggacagat ggtggagcaa gcgtaggcat
     7681 catctgtaga tctcccataa gcataattga taagaatagc taaagtttgc tcaggtaaat
     7741 ggcgaaggga atgctaatca tcagaaatgt tggcaagcgt cacagaattt ttctcatctt
     7801 aaactgttct gaccagtgga ttagcatgtc ttaaggtggt tttgtgaatg attatcgtgc
     7861 tagcgatcat gggttagttc tgcggattca acgcgctaag gataaagtcc accatctgtt
     7921 cgcgataaac atcgccgtga cgatcggaaa aagcatcaat gtgttcgcca tccgggatta
     7981 ggatcaaccg ttttgtctct tttgccaggc tatacaactt ttcgctgtgc tgccatggga
     8041 taacgtgatc agctttaccg tgaatgagta aaagcgggat cgggctgacg ctggcgatat
     8101 aattttcgcc gctgtaactc tcatcaagta agtagccact gccggggatc atctgattgg
     8161 cgatggttgc ataagaggca aatgtggagt cgaggatcac cgcacgtatg ccttcacgat
     8221 catcctgacc aataacagca agaatattcg ccccgccaat actctgcccg aacagcacca
     8281 gccgttgtgg gtttacatca ctgcgatggc gcaccacatt gatggcactt tgcgtatcgt
     8341 ccagcaatcc ggcctgggac ggcgtgcctt ttgatttacc aaacccgcga taatcaaaca
     8401 taaaaacgtt gaaattacgc tcgggtaacc aactgaccag cggccagtgg gcggacatat
     8461 ttccggcatt gccgtgagca tgaatgatgg ttgcgatggc attgtcagca gggcccgtcg
     8521 aagaaggaat aaaccagcct tgcagacgag taccgtcctt agccgtaaat tcgacggatt
     8581 ccgccgacca ggggtcggga ccgtaaattt tatcgtcagg ataatagaag aagttgatgg
     8641 caacgcgcgg cactaaataa acgcagaagg caacaacaaa gagaataaac agaattttga
     8701 gaacgcgttt gttcactggc agcgccataa cctttccttg tgacttcttt atcatccatt
     8761 aatcggggaa agatggtggg agagtgacga aagaaagtaa agcggtgagg aagtatttcg
     8821 gaatagcgcc tgaaaggcga gggcgggtga gtgacatcac ccgcctgagt cattaacgct
     8881 tcagagcgtc gcttaactca tcacgcatgt tcgccagcat ggctttcaca acacgcgggt
     8941 taccagcaac gatgttaccg gtcagcatat agttatgacc accggtgaag tcgctgacga
     9001 tgccgcccgc ttcacgaacc agcagctcgc ctgccgcgaa atcccacgga cgcagaccga
     9061 tttcaaagaa accgtcaaca cgacccgcag cgacgtaagc cagatccagc gccgcagaac
     9121 cggtacgacg gaagtctgca cattcgttga acagtttgcc gacgatgttg atgtaggtag
     9181 tggcgtactg ttttgctttg aacgggaagc cggtcgccag aatggtaccg tcgagatcgc
     9241 gagcggtgct accgcgcaga cggtagccgt tcagctgtgc gccctgaccg cgagtggcgg
     9301 tgaacagttc gttacgcata ggatcgtata ccacagcaac ttcggtgcgg cctttgatac
     9361 gaacggcgat agataccgcg aagtgcggca gacgtttgat aaagttggta gtaccatcca
     9421 gtggatcgat aacccattga acatcctgat cagtaccttc aagttcaccg ctttcttcgg
     9481 tgatgatggt gtgctgtggg taagatttac gaatcgtgtc gataatcacc gcttcgacag
     9541 ctttatctac gttggtcacg aaatcgttac tgcctttctg gctcgcttct acagcgtccg
     9601 gggtttcata gtttttggca attaaattac ccgccttgcg cgctgcgcgc acggcgatgt
     9661 tcagcatcgg atgcatcggt ctctctcact ggatgttaaa gaacgggaaa acggcgcaga
     9721 gtatagcagc gagatcggca tctgtcttcg ttttatgata agatgcgtga ataatcttta
     9781 gatgaagaaa gacaatgctg caaaatattc gaattgtgct ggtggagacg tcacacaccg
     9841 gcaatatggg ttctgttgcc cgtgccatga aaacaatggg attaaccaat ctgtggctgg
     9901 ttaatccact ggtgaaaccc gactcccagg cgattgccct ggcagcaggg gccagcgatg
     9961 tgattggtaa tgctcacatc gtcgatacgc tcgacgaagc gttagccggt tgtagcttgg
    10021 tggtgggcac cagcgcacgt tcccgcacgc tgccatggcc gatgctcgac ccgcgcgaat
    10081 gcggcctgaa aagcgtcgct gaagcggcaa ataccccggt ggcgctggtg tttggtcgcg
    10141 agcgcgtcgg cttgaccaat gaagagttgc agaaatgcca ttatcatgtc gcgattgcgg
    10201 ctaacccgga atacagctcg ctgaacctgg cgatggcggt tcaggttatt gcctatgaag
    10261 tgcgcatggc ctggctggca actcaggaaa acggcgagca ggtcgagcat gaagagacgc
    10321 cgtatccgct ggtcgatgat ctggagcgtt tttatggtca tctggaacaa acgctgctgg
    10381 caaccggttt tatccgtgaa aaccatccgg ggcaggtgat gaataaattg cgccgtctgt
    10441 ttacccgtgc gcgcccggaa agccaggaat tgaatatcct gcgcgggatt ctggcttcta
    10501 ttgagcagca gaataaaggt aacaaggccg aataacagta gttgcctgat gcgacgctta
    10561 acgcgtctta tcaggcctac aatgtacaga accgtaggtc ggataaggtg ttcacaccgc
    10621 atccgacaac aggtacaaac gccacgataa aaaaatggca ctgaaggtta aatacccgac
    10681 taaatcagtc aagtaaatag ttgaccaatt tactcgggaa tgtcagactt gaccctgcta
    10741 ttcaataccc ccacttttac aataaaaaac cccgggcagg ggcgagtttg aggtgaagta
    10801 agacatgaga ctgacatcta aagggcgcta tgccgtgacc gcaatgcttg acgttgcgct
    10861 caactctgaa gcgggcccgg taccgttggc tgatatttcc gaacgtcagg gcatttccct
    10921 ttcttatctg gaacaactgt tttcccgtct gcgtaaaaat ggtctggttt ccagcgtacg
    10981 tggaccaggc ggtggttatc tgttaggcaa agatgccagc agcatcgccg ttggcgaagt
    11041 gattagcgcc gttgacgaat ctgtagatgc cacccgttgt cagggtaaag gcggctgcca
    11101 gggcggcgat aaatgcctga cccacgcgct gtggcgtgat ttgagcgacc gtctcaccgg
    11161 ttttctcaac aacattactt taggcgaact ggttaataac caggaagtgc tggatgtgtc
    11221 tggtcgtcag catactcacg acgcgccacg cacccgcaca caagacgcga tcgacgttaa
    11281 gttacgcgct taataaaaag aattcagaat caggccggag tgctaaatac tccgtaaaca
    11341 cggtcgtaca tccagccggt agcctgattc cttgcattga gtgatgtacg gagtttatag
    11401 agcaatgaaa ttaccgattt atctcgacta ctccgcaacc acgccggtgg acccgcgtgt
    11461 tgccgagaaa atgatgcagt tcatgacgat ggacggaacc tttggtaacc cggcctctcg
    11521 ttctcaccgt ttcggctggc aggctgaaga agcggtagat atcgcccgta atcagattgc
    11581 cgatctggtc ggcgctgatc cgcgtgaaat cgtctttacc tctggtgcaa ccgaatctga
    11641 caacctggcg atcaaaggtg cagccaactt ttatcagaaa aaaggcaagc acatcatcac
    11701 tagcaaaacc gaacacaaag cggtactgga tacctgccgt cagctggagc gcgaaggttt
    11761 tgaagtcacc tacctggcac cgcagcgtaa cggcattatc gacctgaaag aacttgaagc
    11821 agcgatgcgt gacgacacca tccttgtttc catcatgcac gtaaataacg aaatcggcgt
    11881 ggtgcaggat atcgcggcta tcggcgaaat gtgccgtgct cgtggcatta tctatcacgt
    11941 tgatgcaacc cagagcgtgg gcaaactgcc tatcgacctg agccagttga aagttgacct
    12001 gatgtctttc tccggtcaca aaatctatgg cccgaaaggt atcggtgcgc tgtatgtacg
    12061 tcgtaaaccg cgcgtacgca tcgaagcgca aatgcacggc ggcggtcacg agcgcggtat
    12121 gcgttccggc actctgcctg ttcaccagat cgtcggaatg ggcgaggcct atcgcatcgc
    12181 aaaagaagag atggcgaccg agatggaacg tctgcgcggc ctgcgtaacc gtctgtggaa
    12241 cggcatcaaa gatatcgaag aagtttacct gaacggtgac ctggaacacg gtgcgccgaa
    12301 cattctcaac gtcagcttca actacgttga aggtgagtcg ctgattatgg cgctgaaaga
    12361 cctcgcagtt tcttcaggtt ccgcctgtac gtcagcaagc ctcgaaccgt cctacgtgct
    12421 gcgcgcgctg gggctgaacg acgagctggc acatagctct atccgtttct ctttaggtcg
    12481 ttttactact gaagaagaga tcgactacac catcgagtta gttcgtaaat ccatcggtcg
    12541 tctgcgtgac ctttctccgc tgtgggaaat gtacaagcag ggcgtggatc tgaacagcat
    12601 cgaatgggct catcattaat cggtatcgga atcaggagaa tttataatgg cttacagcga
    12661 aaaagttatc gaccattacg agaatccgcg taacgtgggt tcctttgaca acaacgacga
    12721 gaacgtcggc agcggcatgg tgggtgcacc ggcctgtggc gacgtgatga agttgcagat
    12781 taaagtcaac gatgaaggta tcattgaaga cgcgcgtttt aaaacttacg gctgcggttc
    12841 tgctatcgct tccagctccc tggtcaccga atgggtgaaa gggaagtctc tcgtcgaagc
    12901 gcaggcgatc aaaaacaccg atattgctga agaacttgaa ctgccgccgg tgaaaattca
    12961 ctgttctatt ctggcagaag acgcgatcaa agccgccatt gcggactata aaagcaaacg
    13021 tgaagcaaaa taagagttga ggtttggtta tgtcgattac actgagcgac agtgcagcag
    13081 cgcgagtaaa tacctttctg gctaaccgcg gcaaagggtt tggcctgcgt ctgggcgtga
    13141 gaacctccgg atgttcaggt atggcttatg tactggaatt tgttgacgaa ccgacgccgg
    13201 aagacatcgt gtttgaagac aaaggcgtga aagtcgtggt cgatggcaaa agcctgcaat
    13261 ttctggacgg tacgcagctg gacttcgtaa aagaaggcct gaatgaaggg tttaaattca
    13321 ccaacccgaa cgttaaagat gagtgtggtt gcggcgaaag cttccacgtt tgatgcgcat
    13381 accgataacc ccaccgtggt cgcctgcgcg tggggtttgt tttacctgat tcgccgttat
    13441 cgcggcggat cgcagccctg agaatgttat ggattacttc accctctttg gcttgcctgc
    13501 ccgttatcaa ctcgataccc aggcactgag cctgcgtttt caggatctac aacgtcagta
    13561 tcatcctgat aaattcgcca gcggaagcca ggcggaacaa ctcgccgccg tacagcaatc
    13621 tgcaaccatt aaccaggcct ggcaaacgct gcgtcatccg ttaatgcgcg cggaatattt
    13681 gctttctttg cacggttttg atctcgccag cgagcagcat actgtgcgcg acaccgcgtt
    13741 cctgatggaa cagctggagc tgcgcgaaga actggacgag atcgaacagg cgaaagatga
    13801 agcgcggctg gaaagcttta tcaaacgtgt gaaaaagatg tttgataccc gccatcagtt
    13861 gatggttgaa cagttagaca acgagacgtg ggacgcggcg gcggataccg tgcgtaagct
    13921 gcgttttctc gataaactgc gaagcagtgc cgaacaactc gaagaaaaac tgctcgattt
    13981 ttaatttctg gaagctaaac atggccttat tacaaattag tgaacctggt ttgagtgctg
    14041 cgccgcatca gcgtcgtctg gcggccggta ttgacctggg cacaaccaac tcgctggtag
    14101 cgacagtgcg cagcggtcag gccgaaacgt tagccgacca tgaaggccgt cacctgctgc
    14161 catctgttgt tcactatcaa cagcaagggc attcggtggg ttatgacgcg cgtactaacg
    14221 cagcgctcga taccgccaac accattagtt ctgttaaacg cctgatggga cgctcgctgg
    14281 ctgatatcca gcaacgctat ccgcatttgc cttatcaatt ccaggccagc gaaaacggcc
    14341 tgccaatgat tgaaacggcg gcggggctgc tgaacccggt gcgcgtttct gcggacatcc
    14401 tcaaagcact ggcggcgcgg gcaactgaag ccctggcagg cgagctggat ggtgtcgtta
    14461 tcaccgttcc ggcgtacttt gacgatgccc agcgtcaggg caccaaagac gcggcgcgtc
    14521 tggcggggtt gcatgttctg cgcttactta acgaaccgac cgctgcggct atcgcctacg
    14581 ggctggattc cggtcaggaa ggcgtgatcg ccgtttatga cctcggtggc gggacgtttg
    14641 atatttccat tctgcgctta agtcgcggcg tgtttgaagt gctggcaacc ggcggtgatt
    14701 ccgcgctcgg cggcgatgat ttcgaccatc tgctggcgga ttacattcgc gagcaggcgg
    14761 acattcctga tcgtagcgat aaccgcgttc agcgtgaact gctggatgcc gccattgcag
    14821 ccaaaatcgc gctgagcgat gcggactccg tgaccgttaa cgttgcgggc tggcagggcg
    14881 aaatcagccg tgaacaattc aatgaactga tcgcgccact ggtaaaacga accttactgg
    14941 cttgtcgtcg tgcgctgaaa gacgcgggcg tagaagctga tgaagtgctg gaagtggtga
    15001 tggttggcgg ttctactcgc gtgccgctgg tgcgtgaacg ggtaggcgaa tttttcggtc
    15061 gtccaccgct gacttccatc gacccggata aagtcgtcgc tattggcgcg gcgattcagg
    15121 cggatattct ggtgggtaac aagccggaca gcgaaatgct gctgcttgat gtgatcccac
    15181 tgtcgctggg cctcgaaacg atgggcggcc tggtggagaa agtgattccg cgtaatacca
    15241 ctattccggt ggcccgcgct caggatttca ccacctttaa agatggtcag acggcgatgt
    15301 ctatccatgt aatgcagggt gagcgcgaac tggtgcagga ctgccgctca ctggcgcgtt
    15361 ttgcgctgcg tggtattccg gcgctaccgg ctggcggtgc gcatattcgc gtgacgttcc
    15421 aggtcgatgc cgacggtctt ttgagcgtga cggcgatgga gaaatccacc ggcgttgagg
    15481 cgtctattca ggtcaaacct tcttacggtc tgaccgatag cgaaatcgct tcgatgatca
    15541 aagattcaat gagctatgcc gagcaggacg taaaagcccg tatgctggca gaacaaaaag
    15601 tagaagcggc gcgtgtgctg gaaagtctgc acggcgcgct ggctgctgat gccgcgctgt
    15661 taagcgccgc agagcgtcag gtcattgaca atgctgccgc tcacctgagt gaagtggctc
    15721 agggcgatga tgttgacgcc atcgaacaag cgattaaaaa cgtagacaaa caaacccagg
    15781 atttcgccgc tcgccgcatg gaccagtcgg ttcgtcgtgc gctgaaaggc cattccgtgg
    15841 acgaggttta atatgccaaa gattgttatt ttgcctcatc aggacctctg ccctgatggc
    15901 gctgttctgg aagctaatag cggtgaaacc attctcgacg cagcgctgcg taacggtatc
    15961 gagattgaac acgcctgtga aaaatcctgt gcttgcacca cctgccactg catcgttcgt
    16021 gaaggttttg actcgctgcc agaaagctca gagcaggaag acgacatgct ggacaaagcc
    16081 tggggactgg agcccgaaag ccgtttaagc tgccaggcac gcgttaccga cgaagattta
    16141 gtagtcgaaa tcccgcgtta cactatcaac catgcgcgtg agcattaaca gaggttagta
    16201 tgggacttaa gtggaccgat agccgcgaaa ttggcgaagc actgtacgat gcgtatcctg
    16261 atcttgatcc gaaaacggtt cgattcaccg atatgcatca gtggatttgc gatctggaag
    16321 atttcgacga cgacccgcag gcatccaacg agaaaatcct cgaagcgatt ttgttagtct
    16381 ggctggacga ggccgaataa tttccgcgtc atgcttcacg ccgcagatgc gttggctgcg
    16441 ctcgttcacc ccagtcacat agttatctat gctcctgggg cttcactcac ttgccgcctt
    16501 cctgcaacgc gaatcattta gcggaaaaat cctggggctg ccaactggcg gcccttttac
    16561 aaagaaggat aactaaaatg acagaagcga tgaagattac cctctctacc caacctgccg
    16621 acgcgcgctg gggagaaaaa gcaacttaca gcattaataa tgacggcatt accctgcatt
    16681 tgaacggggc agacgatctg gggctgatcc agcgtgcggc gcgcaagatt gacggtctgg
    16741 gcatcaagca tgttcagtta agcggtgaag gctgggatgc ggatcgctgc tgggcattct
    16801 ggcaaggtta caaagccccg aaaggcacgc gtaaagtgga gtggccggat ctggacgatg
    16861 cccagcgcca ggaactggat aaccgcctga tgatcatcga ctgggtgcgt gacaccatca
    16921 acgcaccggc agaagaattg ggaccatcgc aactggcaca gcgtgctgtt gatctgatca
    16981 gcaacgtcgc gggcgatcgt gtgacttatc ggatcaccaa aggcgaagat ctgcgtgagc
    17041 aaggttatat ggggctgcac accgtcggac gcgcttcaga acgttctccg gtattgctgg
    17101 cgctggatta caacccaact ggcgataaag aagcgccagt gtacgcgtgc ctggtaggta
    17161 aaggtatcac ttttgactcc ggcggctaca gcatcaaaca gactgcgttt atggactcga
    17221 tgaagtcgga catgggcggc gcggcaacgg ttaccggggc gctggcattt gccattacgc
    17281 gcggactgaa caagcgcgtg aagctgttcc tctgctgtgc ggataacctg attagcggca
    17341 atgcgttcaa gctgggcgat atcatcacct atcgcaacgg taaaaaagtt gaagtgatga
    17401 acactgatgc cgaagggcgt ctggtgcttg ccgatggtct gattgatgcc agtgcgcaga
    17461 aaccggaaat gatcattgat gcggcgaccc tcaccggggc ggcgaaaact gcgctgggta
    17521 atgattatca cgcgctgttc agttttgacg atgcgcttgc cggtcgtttg ctggcgagtg
    17581 cctcacaaga gaacgaacca ttctggcgtc tgccgctggc ggaattccac cgcagccagc
    17641 tgccgtctaa ctttgccgaa ctgaacaata ccggaagcgc ggcgtatccg gcaggcgcga
    17701 gcacggcggc gggcttcctg tcgcactttg ttgagaacta tcagcaaggc tggctgcata
    17761 tcgactgctc ggcgacttac cgtaaagcgc cggttgaaca gtggtctgcg ggcgctacgg
    17821 gacttggtgt gcgcacgata gctaatctgt taacggcgta aactaatatg ccggatgcgg
    17881 cgtaaacgcc ttatccggcc tacaaattct gcaaattcaa caaattgcga atcccttgta
    17941 ggcctgataa gcgtagcgca tcaggcattt gcgccccgaa cgttgaatga attgtggttc
    18001 ttatgtccga aacaaaaaac gaacttgaag acctgctgga aaaagcagca accgaaccgg
    18061 cacaccgccc ggcctttttc cgtactctac tggaatccac cgtctgggtg cctggtacgg
    18121 cggcgcaggg cgaggctgtg gttgaagata gtgcgcttga tttacagcac tgggaaaaag
    18181 aagacggcac cagcgtcatt ccttttttca cctcgttaga agcgcttcaa caggcggttg
    18241 aagacgaaca ggcatttgtc gtaatgcccg ttcgcacatt gtttgagatg acccttggcg
    18301 aaacgctctt ccttaatgcc aaactgccga ccggtaaaga atttatgccg cgtgaaatca
    18361 gtttgttgat tggtgaagag ggaaatccgc tgagcagcca ggaaatcctg gaaggcggtg
    18421 aatcgctgat attatcggaa gttgcagagc cgccagcaca aatgattgat tcactcacca
    18481 ccttatttaa aaccattaag ccggtgaagc gtgcttttat ttgttcaatt aaagagaacg
    18541 aagaggcaca gcctaattta tttattggca ttgaagccga tggtgatatc gaagaaatta
    18601 ttcaggcgac gggaagtgta gcgaccgata cattacctgg cgatgaacca atcgatattt
    18661 gtcaggtgaa aaaaggggaa aaaggaatta gccactttat taccgaacat attgtgccat
    18721 tctatgaacg tcgctggggc ggttttttgc gtgactttaa acaaaaccgg ataatctaaa
    18781 atatgccgcc ctaaagggcg gcattcgggc ttacttgctg ctctgaaggt tgttaaccac
    18841 cagtaaattg tcgccggaga gactccggtt attatttact tgggaaaaca gcgcaccaga
    18901 aacaataagt acgatacaga agcaccgtat cttcattgtg cggtccatgc gaagaccgca
    18961 tcacgacagc accactggcg atattgccgt tagagcaagc gctgagacgc gcttgctcta
    19021 acggtcgcgg aacatccttt ccttaagcca gcgcccgctt gcgagacggc ggaaagggcc
    19081 gcagtggata atatactaac agaacaataa taactctctt ttgcttgaca aaaagagagt
    19141 tactggcgag tattgtctcc gctgagacgc gcttgcatta ctacggtaat acaacctgct
    19201 ggaaaggccg cgaaccagac cagcaataaa aaaccgccaa attcggcggt tttttattgc
    19261 ctgtaaaata cttatttcac tggctcaacc ggtaaatctg cccgcgcgcc ccattcactc
    19321 catgcgccgt cgtacagttt cacgtttggc acatccagcg tcgcgagtgc taacaaaacc
    19381 acggctgccg ttacaccaga gccgcagctg acgataattg gtttgtcgta gctgacgccg
    19441 cgaccaaaaa atatcgcatc cagttcatcg gtcgttttta gttcgccttc gcgcaccagt
    19501 tccgtccacg gcacattcag tgctccggga atatgtccgc gacgtaaacc tgggcgaggt
    19561 tcatcaactt ctgcgttaaa acgtgcagcc gggcgggcat caataatttg cgccgtattt
    19621 tcatggcttg ccaataatac atcggttact ttcaccacgg cttcaggatt aaacgcggcg
    19681 ttaaactctc cttccggcag ctctactgca ccttcttcta acagcagatc atcgcgctgc
    19741 cagcctgcaa gtccaccccc cagaatcgac actttctcta caccaaaggt gcgcagcatc
    19801 caccatgctc gtggggctga gaaaagat
//
LOCUS       DAJTOR010000068        19763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTOR010000068 DAJTOR010000000
VERSION     DAJTOR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.068"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            41..985
                     /gene="wecH"
                     /locus_tag="OO249_002115"
     CDS             41..985
                     /gene="wecH"
                     /locus_tag="OO249_002115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5636413.1"
                     /translation="MVVMIHTTTWYVTNAHSVSPVTWDIANVLNSASRVSVPLFFMIS
                     GYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINMELALKNLLQKPVFYHLW
                     FFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTVPQKIDGFEWLPINLYIN
                     GDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFIISRGTLYELQWRGNFADT
                     WYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLGIYGFHALIIHALRTRGI
                     ELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(1027..1464)
                     /locus_tag="OO249_002116"
     CDS             complement(1027..1464)
                     /locus_tag="OO249_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5636414.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(1510..1851)
                     /locus_tag="OO249_002117"
     CDS             complement(1510..1851)
                     /locus_tag="OO249_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636415.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(2016..2207)
                     /locus_tag="OO249_002118"
     CDS             complement(2016..2207)
                     /locus_tag="OO249_002118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636416.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(2140..3468)
                     /gene="xylB"
                     /locus_tag="OO249_002119"
     CDS             complement(2140..3468)
                     /gene="xylB"
                     /locus_tag="OO249_002119"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5636417.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGCGASTGRSKAGADCGESREIAH"
     gene            complement(3585..4862)
                     /gene="xylA"
                     /locus_tag="OO249_002120"
     CDS             complement(3585..4862)
                     /gene="xylA"
                     /locus_tag="OO249_002120"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCR5636418.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVSFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGR"
     gene            5228..6199
                     /gene="xylF"
                     /locus_tag="OO249_002121"
     CDS             5228..6199
                     /gene="xylF"
                     /locus_tag="OO249_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5636419.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGG
                     AIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGN
                     GQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6277..7818
                     /gene="xylG"
                     /locus_tag="OO249_002122"
     CDS             6277..7818
                     /gene="xylG"
                     /locus_tag="OO249_002122"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5636420.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            7796..8976
                     /gene="xylH"
                     /locus_tag="OO249_002123"
                     /pseudo
     CDS             7796..8976
                     /gene="xylH"
                     /locus_tag="OO249_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
     gene            9054..10232
                     /gene="xylR"
                     /locus_tag="OO249_002124"
     CDS             9054..10232
                     /gene="xylR"
                     /locus_tag="OO249_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCR5636421.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNREVML
                     "
     gene            complement(10340..11164)
                     /locus_tag="OO249_002125"
     CDS             complement(10340..11164)
                     /locus_tag="OO249_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5636422.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            11484..13514
                     /gene="malS"
                     /locus_tag="OO249_002126"
     CDS             11484..13514
                     /gene="malS"
                     /locus_tag="OO249_002126"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5636423.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            13692..14945
                     /gene="avtA"
                     /locus_tag="OO249_002127"
     CDS             13692..14945
                     /gene="avtA"
                     /locus_tag="OO249_002127"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5636424.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNVFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(15096..15569)
                     /locus_tag="OO249_002128"
     CDS             complement(15096..15569)
                     /locus_tag="OO249_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5636425.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(15671..16519)
                     /gene="yiaJ"
                     /locus_tag="OO249_002129"
     CDS             complement(15671..16519)
                     /gene="yiaJ"
                     /locus_tag="OO249_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5636426.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            16720..17718
                     /gene="yiaK"
                     /locus_tag="OO249_002130"
     CDS             16720..17718
                     /gene="yiaK"
                     /locus_tag="OO249_002130"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5636427.1"
                     /translation="MKVTFEQLKAAFNRALISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            17730..18198
                     /locus_tag="OO249_002131"
                     /pseudo
     CDS             17730..18198
                     /locus_tag="OO249_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            18316..18789
                     /gene="yiaM"
                     /locus_tag="OO249_002132"
     CDS             18316..18789
                     /gene="yiaM"
                     /locus_tag="OO249_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCR5636428.1"
                     /translation="MKKILEVILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            18792..>19763
                     /locus_tag="OO249_002133"
     CDS             18792..>19763
                     /locus_tag="OO249_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5636429.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPDMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSTWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAIL"
BASE COUNT         4987 a         4949 c         4800 g         5027 t
ORIGIN      
        1 aatttactgg attgataacc tgcgagggat agcgtgttta atggtggtga tgattcacac
       61 cactacctgg tatgtgacca atgctcatag tgttagcccc gtcacatggg atatcgccaa
      121 tgttctgaat tctgcctctc gtgtcagcgt gccgctattt ttcatgattt ccggctatct
      181 cttttttggc gaacgcagcg cccagccgcg ccatttcttg cgtatcggct tatgtctgat
      241 tttttatagc gcaatcgcac tgctctacat tgcgctgttt acctccatca atatggagtt
      301 agcgctgaaa aacctgctgc aaaagccagt gttttaccac ttgtggtttt tcttcgcgat
      361 tgcggtgatt tatctggttt caccgctgat tcaggtgaag aacgtcggcg gaaaaatgtt
      421 gctggtgcta atggcggtga ttggcattat cgctaaccca aacacagtgc cgcagaaaat
      481 tgacggtttt gaatggctgc caattaactt atatatcaat ggcgatactt tttactacat
      541 tctgtatggc atgttgggcc gcgctatagg gatgatggac acacagcata aagcactgtc
      601 gtgggtgagc gccgcgctgt ttgcgacggg ggtttttatt atctctcgcg ggacattata
      661 tgaattgcag tggcgcggaa attttgccga tacctggtat ctttactgtg ggccgatggt
      721 ttttatctgc gcaatcgcgc tattgactct ggttaaaaac acgctggata cgcgtaccat
      781 tcgcggactt ggcttaatct cccgccattc attgggtata tacggattcc acgccttgat
      841 tatccatgcg ctgcgcaccc ggggaattga gcttaaaaat tggccaatac tggatattat
      901 ttggatcttt tgcgcgacgt tggcagcgag tttgttactt tctatgctgg tacaacgaat
      961 cgacagaaac agattagtga gttaagtaaa agcccgttca cattggactg accgggctta
     1021 cgtgaattat tcgctgtatt cttcctgggt aacctgtgtt tcttttggcg ggtttattcc
     1081 ggcatcacga atattcttct gcaccgctac tgcaccaaac aagcttaaga agaaagccag
     1141 tccataaaaa cctttttcac tgagtaataa tgtcgcattc cacagaccta ccatcagtaa
     1201 tgcaacagag ataataaaga cagtcaggca ggtcatataa taaatggaag tggtcggtat
     1261 gccttcatac ttgtcccgaa cggtcttttg ataagacgca gcagaaaaca gtcctaacac
     1321 cagtacggca aaataatacc ctttttcatt taactgcatc tctgcattcc atagccctaa
     1381 cagataggta acgataccac caacgagagc tatccatgac acaatactaa aggccggtga
     1441 ataggttgat attttgttgt ccatcacagt attcctttca ttcctgaatg tgtaagagct
     1501 ttatgttgct cataccgagc gaaacaaacg catttgacca aacaagcaga caaaaaaggc
     1561 gatggggtag atcaccattt cataagcatt gataggtgca tttaaaacac cgataaacaa
     1621 gagaccgata gtgattaacg caactaactg gcagatatgg gtaaaaaaat tatccagttg
     1681 cccgagtttt tctgcgcgaa gatatgcata gttaccaaaa gttgctgtca ttaacacgcc
     1741 aagaaaatag ccttttccac ttagcaatgg gcatgagatc catagcccaa ccagataaac
     1801 cagcgccccg acaacaaata atagttttgc cacagttttt gaggttttca taacgatctc
     1861 catatctacc agcgatacat tacgagtaac caacgaaaga caaaactgaa aaatgccatt
     1921 aacaaatgat tttcagaata aattcatact aaatattaat tagtgagata tatagatgtg
     1981 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
     2041 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
     2101 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
     2161 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc cccccgtacg
     2221 gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact cactacgcgc
     2281 cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca cgacatccat
     2341 gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt cattggggcc
     2401 atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt gtggtgtacg
     2461 ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag cctgttgagc
     2521 tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcgaccc aatccagaca
     2581 cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg catggcaaaa
     2641 gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa aatagacccc
     2701 cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac caactgcacc
     2761 agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc acgctttcgc
     2821 aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcgg gcatctggtc
     2881 acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg cgacatccag
     2941 ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca tacgcagacg
     3001 caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg gctcatgccg
     3061 ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc cggtaatcac
     3121 tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc gcccgtcgtt
     3181 ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg ctccatgcat
     3241 ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc ccagagcttt
     3301 cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc agagtggatg
     3361 cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct gctcgttgag
     3421 caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat cgatcgttcc
     3481 ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac agttagccgt
     3541 tatttgtcga acagataatg atttaccaga ttttccagtt gctcctagcg accactctga
     3601 tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag tgacatttgg
     3661 cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg cgcgatgcgt
     3721 ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag tgccatcgta
     3781 tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg acgacgtact
     3841 ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat ttcatacatc
     3901 accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc cagttgcgca
     3961 tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt ggctatttca
     4021 tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag tttaatctct
     4081 ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta atcatattga
     4141 tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctggaa gccgatttta
     4201 tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc ctgacgcaag
     4261 tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata gttttcaccg
     4321 cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca gctgaagact
     4381 tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa gcagttagcg
     4441 gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac atcaaccatt
     4501 tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac atccacatcg
     4561 tggaagcaat aaaatgacac atgtaacttg tggaaaaact caaatgcgac atctgcttta
     4621 cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa cgcccccaca
     4681 ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt aacaggcggc aaaacgcaag
     4741 tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg gaatgctaac
     4801 gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa ataggcttgc
     4861 atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta atattgggca
     4921 ctccctttca gttgctcaat tatgttattt cacactgcta ttgagataat tcacaagtgt
     4981 gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga agataaaaat
     5041 gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag aaataaacca
     5101 aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt tgctaaaaat
     5161 tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact acagaaggcc
     5221 ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc tgcttaccaa
     5281 cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc tacgtcttga
     5341 acgctggcaa aaagatcgag atatttttgt gaaaaaggct gaatctctcg gtgcgaaagt
     5401 atttgtacag tctgcaaatg gcaatggaga aacacaaatg tcgcagattg aaaacatgat
     5461 taaccggggt gtcgatgttc ttgtcattat tccatataac ggccaggtat taagtaacgt
     5521 tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta tgattaacga
     5581 tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac tgcaggcaaa
     5641 agccctggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct cgccggtaga
     5701 taacaacgcc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt acgttgattc
     5761 cggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg aaaacgcgct
     5821 aaccgccaat aataacaaaa ttgatgctgt agttgcctca aacgatgcca ccgcaggtgg
     5881 ggcaattcag gcattaagcg cgcaaggttt atcagggaaa gtagcaattt ccggccagga
     5941 tgcggatctc gcaggtatta aacgtattgc tgccggtacg caaactatga cggtgtataa
     6001 acctattaca ttgctggcaa atactgccgc agaaattgcc gttgaattgg gcaatggtca
     6061 ggagccaaaa gcagatacct cactgaataa tggcctgaaa gatgtcccct cccgcctcct
     6121 gacaccgatc gatgtgaata aaaacaacat caaagatacg gtaattaaag acggattcca
     6181 caaagagagc gagctgtaag cgttacgccc cggcgcggag cgggggcgtg atttctctcc
     6241 atgccgcgtg aatgaattgg cttaggtgga gtcgttatgc cttatctact tgaaatgaag
     6301 aacattacca aaaccttcgg cagtgtgaag gcgattgata acgtcagctt gcggttgaat
     6361 gctggcgaaa tcgtctcact ctgtggggaa aatgggtctg gtaaatcaac gctgatgaaa
     6421 gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg aaattatttt tgcgggagaa
     6481 gagattcagg cgagtcacat ccgcgatacc gaacgcaaag gtatcgccat cattcaccag
     6541 gaattggccc tggtgaaaga attgaccgtg ctggaaaata tcttcctggg taacgaaata
     6601 acccacaatg gcattatgga ttatgacctg atgacgctac gctgtcagaa gctgctcgca
     6661 caggtcagtt tatccatttc acctgatacc cgcgttggcg atttagggct tgggcaacaa
     6721 caactggttg aaattgccaa ggcacttaat aaacaggtgc gcttgttaat tctcgatgaa
     6781 ccgacagcct cattaactga gcaggaaacg tcggttttac tggatattat tcgcgatcta
     6841 caacagcacg gtatcgcctg tatttatatt tcgcacaaac tcaacgaagt caaagcgatt
     6901 tccgatacga tttgcgttat tcgcgacggt cagcacattg gtacgcgtga tgctgccgga
     6961 atgagtgaag acgatattat caccatgatg gtcgggcgag agttaaccgc gctttaccct
     7021 aatgaaccac ataccaccgg agatgaaata ttacgtattg aacatctgac ggcatggcat
     7081 ccggttaatc gtcatattaa acgagttaat gatgtctcgt tttccctgaa acgtggcgaa
     7141 atactgggta ttgccggact cgttggtgcc ggacgtaccg agaccattca gtgcctgttt
     7201 ggcgtgtggc ccggacaatg ggaaggaaaa atttatattg atggcaaaca ggtagatatt
     7261 cgtaactgtc agcaagccat cgcccagggg attgcgatgg tacccgaaga cagaaagcgc
     7321 gacggcatcg ttccggtaat ggcggttggt aaaaatatta ccctcgccgc actcaataaa
     7381 tttaccggtg gcattagcca gcttgatgac gcggcagagc aaaaatgtat tctggaatca
     7441 atccagcaac tcaaagttaa aacgtcgtcc cccgatcttg ctattggacg tttgagcggc
     7501 ggcaatcagc aaaaagcgat cctcgcccgc tgtctgttac tcaacccgcg cattctcatt
     7561 cttgatgaac ctaccagggg tattgatatt ggcgcgaaat atgagatcta caaattaatt
     7621 aaccaactcg tccagcaggg tattgccgtt attgtcatct cttccgaatt acctgaagtg
     7681 ctcggcctta gcgatcgtgt actggtgatg catgaaggga aactaaaagc caacctgata
     7741 aatcataacc tgactcagga gcaggtgatg gaagccgcat tgaggagcga acatcatgtc
     7801 gaaaagcaat ccgtctgaag tgaaattggc cgtaccgaca tccggtggct tttccgggct
     7861 gaaatcactg aatttgcagg tcttcgtgat gattgcagct atcatcgcaa tcatgctgtt
     7921 ctttacctgg accaccgatg gtgcctactt aagcgcccgt aacgtctcca acctgttacg
     7981 ccagaccgcg attaccggca tcctcgcggt aggaatggtg ttcgtcataa tttctgctga
     8041 aatcgacctt tccgtcggct caatgatggg gctattaggt ggcgtcgcgg cgatttgtga
     8101 cgtctggtta ggctggcctt tgccacttac catcattgtg acgttggttc tgggactgct
     8161 tctcggtgcg tggaacggat ggtgggtcgc gtaccgtaaa gtcccttcat ttattgtcac
     8221 cctcgcgggc atgttggcat ttcgcggcat actcattggc atcaccaacg gcacgactgt
     8281 atcccccacc agcgccgcga tgtcacaaat tgggcaaagc tatctccccg ccagtaccgg
     8341 cttcatcatt ggccgcttgg cttaatggct tttgttggtt ggcaatggcg cggaagaatg
     8401 cgccgtcagg ctttgggttt acagtctccg gcctctaccg cagtagtcgg tcgccaggct
     8461 ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga atgattaccg tggcgttccc
     8521 actcctgttc tgctgctgac gttgctgtta ctcggcggaa tgtttatggc aacgcggacg
     8581 gcatttggac gacgcattta tgccatcggc ggcaatctgg aagcagcacg tctctccggg
     8641 attaacgttg aacgcaccaa acttgccgtg ttcgcgatta acggattaat ggtagccatc
     8701 gccggattaa tccttagttc tcgacttggc gctggttcac cttctgcggg aaatatcgcc
     8761 gaactggacg ctattgcagc atgcgtgatt ggcggcacca gcctggctgg cggtgtggga
     8821 agcgttgccg gagcagtaat gggggcattt atcatggctt cactggataa cggcatgagt
     8881 atgatggatg taccgacctt ctggcagtat atcgttaaag gtgcgattct gttgctggca
     8941 gtatggatgg actccgcaac caaacgccgt tcttgatttt gataaaaatt ttctcaaagc
     9001 cggttacgta ttaccggttt tgagtttttg catgattcag caggaaaaga accatgttta
     9061 ctaaacgtca ccgcatcaca ttactgttca atgccaataa agcctatgac cggcaggtag
     9121 tagaaggcgt aggggaatat ttacaggcgt cacaatcaga atgggatatt ttcattgaag
     9181 aagatttccg cgcccgcatt gataaaatca aggactggtt aggagatggc gtcattgccg
     9241 acttcgacga caaacaaatc gagcaagcgc tggctgatgt cgacgtcccc attgttgggg
     9301 ttggcggctc gtatcacctt gcagaaagtt acccacccgt tcattacatt gccaccgata
     9361 actatgcgct ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg
     9421 ctttttatgg tcttccggaa tcaagcggca aacgttgggc cactgaacgc gaatatgcat
     9481 ttcgtcagct tgtcgccgaa gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg
     9541 cgccagagaa ctggcaacac gcgcaaaatc ggctggcaga ctggctacaa acgctgccac
     9601 cgcaaaccgg gattattgcc gttactgacg cccgggcgcg gcatattctg caagtatgtg
     9661 aacatctaca cattcccgta ccggaaaaat tatgcgtgat tggcatcgac aacgaagaac
     9721 tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg cggcaaatgg
     9781 gctatcaggc ggcaaaactg ttgcatcgat tattagataa agaagaaatg ccgctacagc
     9841 gaattttggt cccaccagtt cgcgtcattg aacggcgctc aacagattat cgctcgctga
     9901 ccgatcccgc cgttattcag gccatgcatt acattcgtaa tcacgcctgt aaagggatta
     9961 aagtggatca ggtactggat gcggtcggga tctcgcgctc caatcttgag aagcgtttta
    10021 aagaagaggt gggtgaaacc atccatgcca tgattcatgc cgagaagctg gagaaagcgc
    10081 gcagtctgct gatttcaacc accttgtcga tcaatgagat atcgcaaatg tgcggttatc
    10141 catcgctgca atatttctac tctgttttta aaaaagcata tgacacaacg ccaaaagagt
    10201 atcgcgatgt aaatcgtgag gtcatgttgt agcgatgatg agaattgtcg gcgtcacatc
    10261 agggaactca gcacagccac ctgatgcgag caaaagcctt atccgaccga caaatcagat
    10321 aaggctcaag gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta tcctggtaca
    10381 ttgcgaacag gtagttatta taactcttcc ctttggtcga gtagcccttc agcttgtgaa
    10441 tcatcgctgt ggcagtcact tcctgatccg ctttacgcag ctgcgcacgc gatttacgga
    10501 acgaagagta agccgggtgc gtattcaggt tagtgacata ggcgctcacc gattctttga
    10561 cagaactaaa ctgtgagtac cctttcactt tacccggcgc attggtacaa cgtcctttca
    10621 tgcatttcat gccgaacaga ttgttattgt tgcgcgccag cttcgacgtt ccccaaccgc
    10681 tttctgctgc agccatcgtc gccaccatac tggtagggat aatgtctacg cgttcaagca
    10741 aggtattcca cgggattttt cgcgtattac cagaccactt caccttgtag cgtttggcga
    10801 tgtctttcag acgcgcacgc tcagcaggtg accattggcc ctgatactgt tttgaaatca
    10861 gccagttacg ttctgcagta atggctgcat tttggctggt aatgtaaggc attacggtcc
    10921 ggagaaacgc ctttttcctt ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac
    10981 tactctttgc actattgcga gaatactctt gtttactgct tacctgttta ttactagctt
    11041 tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg
    11101 aaaacaccag agtgagtaac ataagaatca tcgccccata tcgtcgtatg ggagtcaaaa
    11161 tcatcaggtc tcctggtcgg atttaatcat tccaacacct tatatttttc acaaatttga
    11221 gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag caccccaagg aatagtccaa
    11281 atctgaaact atgtcacgag ttaacgattc agattggcgc taaatcgcag aaaatgtggg
    11341 gtttatcgca aaattcagcc gttttttgcg cgcgatcgct cacccttgct tctcatcctg
    11401 tggacttacc gctcagggat gagttttgtc tggcttatcg ctggcaaact gtctgaaatc
    11461 gcagcaataa ggactcatcc accatgaaac tcgcctcctg ttttctgaca ctccttcctg
    11521 gcttcgccgt tgccgccagc tggacttctc cggggttccc tgcctttagc gaacagggaa
    11581 cgggaacatt tgtcagccac gcgcagttgc ccaaaggtac gcgtccactc acgctaaatt
    11641 ttgaccaaca gtgctggcag cctgcagatg cgataaaact caatcagatg ctttccctgc
    11701 aaccttgtag caacacgccg cctcaatggc gattgttcag ggacggcaaa tatacgctgc
    11761 aaatagacac ccgctccggt acgccaacat tgatgatttc catccagaac gccgccgaac
    11821 cggtagcaaa cctggtccgt gaatgcccga aatgggatgg attaccgctc acgctggatg
    11881 tcagcgccac tttcccggaa ggagccgccg tacgggatta ttacagccag caaattgcga
    11941 tagtgaagaa cggtcaaata acgttacaac ccgctgctac cagcaacggt ttactcctgc
    12001 tggaacgggc agaaactgac acatccgccc ctttcgactg gcataacgcc acggtttact
    12061 ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa tgaccagagt tacggacgtc
    12121 ataaagacgg tatggcggaa attggcactt ttcacggcgg cgatttacgc ggcctgatca
    12181 acaaactgga ttacctccag cagttgggcg ttaatgcttt atggataagc gccccatttg
    12241 agcaaattca cggctgggtc ggcggcggta caaaaggcga tttcccgcat tatgcctacc
    12301 acggttatta cacacaggac tggacgaatc ttgatgccaa tatgggcaac gaagccgatc
    12361 tacggacgct ggttgatagc gcacatcagc gcggtattcg tattctcttt gatgtcgtga
    12421 tgaaccacac cggctatgcc acgctggcgg atatgcagga gtatcagttt ggcgcgttat
    12481 atctttctgg tgacgaagtg aaaaaaacgc tgggtgaacg ctggagcgac tggaaacctg
    12541 ccgccgggca aacctggcat agctttaacg attacattaa tttcagcgac aaaacaggct
    12601 gggataaatg gtggggaaaa aactggatca gaacggatat cggcgattac gacaatcctg
    12661 gattcgacga tctcactatg tcgctggcct ttttgccgga tatcaaaacc gaatcaacta
    12721 ccgcttctgg tctgccggtg ttctataaaa acaaaacgga tactcacgct aaagtcatcg
    12781 aaggctttac acctcgcgat tacttaaccc actggttaag tcagtgggtc cgcgactatg
    12841 ggattgatgg ttttcgggtc gataccgcca aacatgttga gttgcccgcc tggcagcaac
    12901 tgaaaaccga agccagctcc gcgcttcgcg aatggaaaaa aactaacccc gacaaagcat
    12961 tagatgacaa acctttctgg atgaccggtg aagcctgggg ccacggcgtg atgcaaagtg
    13021 actactatcg ccacggcttc gatgcgatga tcaatttcga ttatcaggag caggcggcga
    13081 aagcagtcga ttgtctggcg cagatggata cgacctggca gcaaatggcg gagaaattgc
    13141 agggtttcaa cgtgttgagc tacctctcgt cgcatgatac ccgtctgttc cgtgaagggg
    13201 gcgacaaagc agcagagtta ttactattag cgccaggcgc ggtacaaatc ttttatggcg
    13261 atgaatcctc gcgtccgttc ggtcctacag gttctgatcc gctgcaaggt acacgttcgg
    13321 atatgaactg gcaggatgtt agcggtaaat ctgccgccaa cgtcgcgcac tggcagaaaa
    13381 tcagccagtt ccgcgcccgc catcccgcaa ttggcgcggg caaacaaacg acactttcgc
    13441 tgaagcaggg ctacggcttt gttcgtgagc atggcgacga taaagtgctg gtcatctggg
    13501 ctgggcaaca gtgacttttc cggcttcccg ttcatcagta cctcgggaag ccgccaacca
    13561 ggataaaatg tcagccctaa tcagcgttgc aggataaagc accgctcact cttcaacaga
    13621 ccgatttgca ccccagcaaa tgtagcgtta ttgttacctt ccttgctaca gagttcgaca
    13681 gatatcccgc tatgacattc tccctttttg gtgacaaatt tacccgccac tccggcatta
    13741 cgctgctgat ggaagatctg aacgacggtt tacgcacgcc tggcgcgatt atgctcggcg
    13801 gcggtaatcc ggcgcagatc ccggaaatgc aggactactt ccagacgcta ctgaccgaca
    13861 tgctggaaag tggcaaagcg actgatgcac tgtgtaacta cgacggtcca caggggaaaa
    13921 cggagctact cacactgctt gccggaatgc tgcgcgagaa gttgggttgg gatatcgaac
    13981 cacagaatat tgcactaaca aacggcagcc agagcgcgtt tttctactta tttaacctgt
    14041 ttgccggacg ccgtgccgat ggtcgggtca aaaaagtgct gttcccgctg gcaccggaat
    14101 acattggcta cgctgacgcc ggactggaag aagatctgtt tgtctctgcg cgtccgaata
    14161 ttgaactgct gccggaaggc cagtttaaat accacgtcga ttttgagcat ctgcatattg
    14221 gcgaagaaac cgggatgatt tgcgtctccc ggccgacgaa tccaacaggc aatgtgatta
    14281 ctgacgaaga gttgctgaag cttgacgcgc tggcgaatca acacggcatt ccgctggtga
    14341 ttgataacgc ttatggcgtc ccgttcccgg gtatcatctt cagtgaagcg cgcccgctat
    14401 ggaatccgaa tatcgtgctg tgcatgagtc tttccaagct ggggctgcct ggctcccgct
    14461 gcggcattat catcgccaat gaaaaaatca tcactgccat caccaatatg aacggcatta
    14521 tcagcctggc acctggcggt attggtccgg cgatgatgtg tgaaatgatt aagcgtaacg
    14581 atctgctgcg cctgtctgaa acagtcatca aaccgtttta ctaccagcgt gttcaggaaa
    14641 ctatcgccat cattcgccgc tatttaccgg aaaatcgctg cctgattcat aaaccggaag
    14701 gagccatttt cctctggcta tggtttaagg atttgcccat tacgaccgag cagctctatc
    14761 agcgcctgaa agcacgcggc gtgctgatgg tgccggggca caacgtcttc ccgggactgg
    14821 ataaaccgtg gccgcatacg catcaatgta tgcgcatgaa ctacgtacca gagccggaga
    14881 aaattgaggc gggggtgaag attctggcgg aagagataga aagagcctgg gctgaaagtc
    14941 actaaaaatt gcctgatgcg ctacgcttat caggcctaca tgatttctgc aatatattga
    15001 atttggaaga atttgtaggc cggataaggc gtttacgccg catctggcat aaacaaagcg
    15061 cactattccg gcgttgagaa acgccggaaa acgtttcaaa caggctgctg ccgtagccga
    15121 tgccgctgca ctttctcgac atccatgcac tgcaacgcct ttgtcgggca ggcttcaaca
    15181 caggccggcc ccgtctcccg atgccagcac agatcgcact taatcgccct cgcctttttg
    15241 cgcgaagaaa cgacctccat cgcaccaaac gggcaagcca gcatacagct tttacagcca
    15301 atgcaacgtg tttgttcaac gaaaatatgc ccatgttcgc ggcttatcgc gtcaacaggg
    15361 cagacattcg cgcacggtgc atcttcacac tgatgacagg ctactgccgt ggtccagctg
    15421 tggtctttaa tgacacgaat acgggaaata aactcgtctg gtgacaacgc agcgcaatcc
    15481 tgattctcct gatgcgacac tgcgcaagcc acttcacagg tacggcaacc gatacatttc
    15541 gtcgcatccg caataataaa ccggttcatg atcactcctt tgcactaaaa cagcagagtg
    15601 ccagagagcg cgtccctact gctttgatcc aacgaaggaa atgttaaagt tttggcactt
    15661 gtccaaagcg ttatgtgatc gcgcccaggt catccctgac agtaaatccc agttcattag
    15721 aaatagcctg cgcggtttca cgcagtggtt tcaggagatt tttctctccc acctgtttca
    15781 gacgtgatgt cgaaagcgaa atcgacacgg cgtacggcac ccgcccatga atatcaaaca
    15841 ccggaacagc aatacaggag acgccgagtt cgttttcttc tctgtccatc gccgctccgc
    15901 tttcacgaat gtgcgccagt tcgtcgaaca tcgcgggcag ctcggtaatg gtattgcggg
    15961 ttaacggctg gatctcatgc tgatggtttt cccagtatga cttcacgtag tccgggtgac
    16021 caaacgccat gtagatcttg cccattgcgg aacagtagag cggcatatgc tggccaatat
    16081 aggcacgggt tcgcagcatc ccggttgtgg gttccagctt ataaatcaaa atagcgtgat
    16141 cgtcttcgcg gctggagaag ttaatggttt caccagtggc gatgttcagt gcctcaagat
    16201 gcggagcggc gatatgaatg atattcagcg aagacagcgc cttctgcccg acggcaataa
    16261 atttggtagt caggcggtaa ctccctgcgg cgggcgcggt ggtcacatag ccgcaggact
    16321 gtaatccctg caataagcga tggacggtac tcttatttaa accagccagc tccgaaagat
    16381 gcgccaacgg acaaccgttt ggatagttgc tcaaaatctc aatcagcatc aacccgcgaa
    16441 acagactctg gcttccggct ggacgctctt tttcctgcgc catctcgttc tctttttttc
    16501 ccatcacttc tttccccatt ttgtcgcgtc ctgatggtag cgcaaagtgt gccgtagttc
    16561 acgatctcga cagataattt ataaccactt gatttttatg tcttttgaaa ttcatcaatc
    16621 agattgcttt gttaaaaagt gatcgatata tttgaaatca agtttcgcat attgaaattt
    16681 taagccaaaa aagcgatcaa aaaaacaagg aagcctggga tgaaagtgac atttgagcag
    16741 ttaaaagcag cctttaatcg ggccttaatt tcacgcggcg ttgacagcga aacggctgac
    16801 gcctgtgcag agatgttcgc ccgcaccacc gaatccggcg tttattctca cggcgttaat
    16861 cgtttccctc gtttcattca acaactggaa aacggcgata tcattcctga tgcccaaccc
    16921 aaacgtataa ccagcctcgg cgcaattgaa cagtgggatg cccagcgttc gatcggcaac
    16981 ctgacggcga aaaagatgat ggatcgcgcc attgaactgg ctgccgatca cggtattggt
    17041 ctggtggcac ttcgtaatgc caaccactgg atgcgcggcg gcagctacgg ctggcaggcg
    17101 gcggaaaaag gctatattgg catttgctgg accaactcca tcgccgtaat gccgccgtgg
    17161 ggcgcaaaag agtgtcgcat tggcactaac ccgctgatcg tcgccattcc ttccacgccg
    17221 atcaccatgg tcgatatgtc gatgtcgatg ttctcttacg gcatgttaga agttaaccgc
    17281 ctggcgggcc gtcagctccc ggtcgatggt ggctttgatg atgagggcaa tttgaccaaa
    17341 gaacctggcg ttatcgagaa gaatcgccgc attttgccga tgggctactg gaaaggttct
    17401 ggcatgtcga ttgtgctgga tatgatcgct actctccttt ccgacggcgc atcggttgcc
    17461 gaagtcaccc aggacaacag cgacgaatac ggcatttcac aaatttttat tgccattgaa
    17521 gtggacaagc ttatcgacgg tcccacccgc gatgccaagc tgcaacgcat catggattac
    17581 gttactaccg ctgaacgcgc tgacgaaaac caggccatcc gcttacccgg ccacgaattt
    17641 actaccctgc tggccgaaaa ccgccgtaac ggcatcactg ttgatgacag cgtgtgggcc
    17701 aaaatccagg cgttatgagg agataagtca tgatttttgg acatatcgcg cagcccaatc
    17761 cgtgccgttt gcccgccgcc attgaaaagg cgctcgattt tctgcgcgct accaacttca
    17821 acgtcctgga gccgggcgtt gtcgaaatcg acggcaagaa tatttatgcg caaattatcg
    17881 atttaaccac acgcgaagcg gtggaaaacc gtccggaagt gcatcgtcgg tatatcgata
    17941 ttcagtttct ggcgtggggt caagagaaaa tcggcattgc tattgataca ggaaataata
    18001 aagtcagcga atcactatta gagcagcgcg atattatttt ttatcatgtc agtgaacatg
    18061 aatcatttat agaaatgata ccgggcagct acgctatttt cttcccgcag gatgttcacc
    18121 gaccaggttg tattctgcaa acaggcctct gagatacgaa aaattgtggt taaagtcgca
    18181 ttaacagcgc tgaattaatc aaataatcgt ctacgccaga acgactgggt aatgttattg
    18241 ctctaactat attaataatt taggttgctg cacatgcaac ttgaattatg acagacatag
    18301 cctcaaggaa tagctatgaa aaaaatactt gaagtaatac tggcgattaa tctcgccgta
    18361 ctttcctgta ttgtgtttat aaacattatt ttaagatatg gttttcagac aagcatttta
    18421 tctgtagatg aattgtcacg ttatttattt gtctggttaa catttattgg cgcgattgtg
    18481 gcttttatgg ataacgccca cgttcaggtc acctttctgg tggagaaact ctcccccgca
    18541 tggcaacgac gagtcgctct ggttacccat tccttaatct tatttatttg tggcgcactg
    18601 gcctggggcg caacactaaa aacaattcag gactggagcg attattcacc gatcctcggt
    18661 ttgcccatcg gcctgatgta tgccgcttgt ctgcccacca gcctcgttat cgcattcttt
    18721 gaactgcgtc atttatatca actcatcacg cgtagcaatt ctctaacgtc gccaccgcaa
    18781 ggagcttaat catggctgtg ctgatttttc tgggctgtct gttgggtggt attgctatcg
    18841 gtttgcctat tgcctgggca ctgttgttgt gcggggcggc gttaatgttc tggctggaca
    18901 tgtttgatgt ccagatcatg gcacaaacgc tggtgaacgg agccgatagc ttctccctgc
    18961 tggcgattcc gttctttgtg ctggcgggtg aaatcatgaa tgcgggcggc ttgtcaaaac
    19021 gcattgttga cctgccgatg aaactggtgg ggcataaacc aggcgggctg ggctacgtcg
    19081 gcgtgctggc agctatgatt atggcgagcc tttctggttc tgccgttgcg gataccgccg
    19141 ccgttgccgc tctactggtg ccgatgatgc gcagcgccaa ctacccggtc aaccgggcgg
    19201 cggggctgat tgcctctggc ggcattatcg cgccaattat tcccccttcc attccgttta
    19261 ttatcttcgg cgtttccagc ggattatcca tcagtaagct gtttatggcg ggcattgccc
    19321 ctgacatgat gatgggcgca acgctgatgc ttacctggtg gtggcaggcc agtcgcctta
    19381 atttaccccg ccagcaaaaa gcaacgatgc aggaaatctg gcactctttt gtctccggta
    19441 tctgggctct gtttcttcca gtcatcatta ttggcgggtt tcgctccggt ctgtttaccc
    19501 ctaccgaagc cggggccgtt gctgctttct atgcgctgtt tgtcgccaca gttatttacc
    19561 gtgaaatgac cttcgccaca ctttggcatg tactcattgg cgcggcgaaa accacctcag
    19621 tggttatgtt tctggtggcc tcggcacaag tttccacctg gctgattacc attgctgaac
    19681 tgccgatgat ggtttctgat ttactgcaac cgttggtcga ttcaccgcgt ctgctgttta
    19741 tcgtcattat ggtggctatc ctg
//
LOCUS       DAJTOR010000069        19366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTOR010000069 DAJTOR010000000
VERSION     DAJTOR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.069"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1230
                     /locus_tag="OO249_002134"
     CDS             <1..1230
                     /locus_tag="OO249_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5636430.1"
                     /translation="FFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSS
                     LESAWKYIIICTVDVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLML
                     LAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIIC
                     QAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPL
                     GIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALA
                     LAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQA
                     VNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTWH
                     DFLPSGTASVSEKHSER"
     gene            1220..2935
                     /gene="hyfG"
                     /locus_tag="OO249_002135"
     CDS             1220..2935
                     /gene="hyfG"
                     /locus_tag="OO249_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR5636431.1"
                     /translation="MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            2945..3484
                     /gene="hyfH"
                     /locus_tag="OO249_002136"
     CDS             2945..3484
                     /gene="hyfH"
                     /locus_tag="OO249_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5636432.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            3481..4239
                     /gene="hyfI"
                     /locus_tag="OO249_002137"
     CDS             3481..4239
                     /gene="hyfI"
                     /locus_tag="OO249_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5636433.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            4232..4645
                     /gene="hyfJ"
                     /locus_tag="OO249_002138"
     CDS             4232..4645
                     /gene="hyfJ"
                     /locus_tag="OO249_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5636434.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHRSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            4675..6687
                     /gene="hyfR"
                     /locus_tag="OO249_002139"
                     /pseudo
     CDS             4675..6687
                     /gene="hyfR"
                     /locus_tag="OO249_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            6709..7554
                     /locus_tag="OO249_002140"
                     /pseudo
     CDS             6709..7554
                     /locus_tag="OO249_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(7592..8653)
                     /locus_tag="OO249_002141"
     CDS             complement(7592..8653)
                     /locus_tag="OO249_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5636435.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            8866..10329
                     /gene="bepA"
                     /locus_tag="OO249_002142"
     CDS             8866..10329
                     /gene="bepA"
                     /locus_tag="OO249_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5636436.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSTPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            10350..10709
                     /gene="arsC"
                     /locus_tag="OO249_002143"
     CDS             10350..10709
                     /gene="arsC"
                     /locus_tag="OO249_002143"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5636437.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(10847..11593)
                     /gene="hda"
                     /locus_tag="OO249_002144"
     CDS             complement(10847..11593)
                     /gene="hda"
                     /locus_tag="OO249_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5636438.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11643..12932)
                     /gene="uraA"
                     /locus_tag="OO249_002145"
     CDS             complement(11643..12932)
                     /gene="uraA"
                     /locus_tag="OO249_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5636439.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(13018..13644)
                     /gene="upp"
                     /locus_tag="OO249_002146"
     CDS             complement(13018..13644)
                     /gene="upp"
                     /locus_tag="OO249_002146"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5636440.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            13968..15005
                     /gene="purM"
                     /locus_tag="OO249_002147"
     CDS             13968..15005
                     /gene="purM"
                     /locus_tag="OO249_002147"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5636441.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            15005..15643
                     /gene="purN"
                     /locus_tag="OO249_002148"
     CDS             15005..15643
                     /gene="purN"
                     /locus_tag="OO249_002148"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5636442.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKIHENAAWLDGQRLPPQGYAADE"
     gene            15815..17881
                     /gene="ppk1"
                     /locus_tag="OO249_002149"
     CDS             15815..17881
                     /gene="ppk1"
                     /locus_tag="OO249_002149"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5636443.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNPEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            17886..>19366
                     /gene="ppx"
                     /locus_tag="OO249_002150"
     CDS             17886..>19366
                     /gene="ppx"
                     /locus_tag="OO249_002150"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5636444.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYW"
BASE COUNT         4672 a         5068 c         5056 g         4570 t
ORIGIN      
        1 ttcttccatc tgtttttgtt caccatgctg ctggttgtta ccagcaataa cctgattgtg
       61 atgtgggcgg cgatcgaagc caccacctta agctcggcgt ttctggtagg catttacggt
      121 cagcgttcat cgctggaatc agcatggaag tacatcatta tttgtactgt tgatgtcgct
      181 tttggtctgt tcggtaccgt gctggtatac gccaacgccg ccagcgttat gccgcaggca
      241 gaaatggcga tattctggag cgaggttctt aagcaatcgt ccttgcttga cccaacatta
      301 atgctgttgg cctttgtgtt tgtgctaatt ggctttggta ccaaaaccgg gctatttccc
      361 atgcacgcct ggctgccgga tgctcacagt gaagcgccga gtccggtcag cgccctgctc
      421 tccgccgtat tgctgaactg cgcgctgttg gtgctgattc gctattacat cattatttgc
      481 caagccatcg gcagcgattt ccccaaccgg ttgttgctca tcttcggcat gttgtcggtt
      541 gccgtggcgg catttttcat tctggtacag cgggacatta agcgtctgct ggcgtactcc
      601 agcgtggaga acatggggct ggtcgcggtg gcgctaggca ttggcgggcc gctgggaatt
      661 tttgccgcgc tgctgcacac cttaaaccac agtctggcaa aaacgctgct gttctgcggt
      721 tccggcaatg tactgctcaa gtacggcacg cgcgatctca acgtcgtctg tgggatgctc
      781 aaaatcatgc catttaccgc cgtgctgttt ggcggcggtg cgctggcgct ggcagggatg
      841 ccgcccttca acatttttct tagcgaattt atgaccgtta ccgcaggact ggcacgtaat
      901 cacctgctga ttatcgtcct gctgttattg ctgttaacgc tggtgctggc gggcctagta
      961 cggatggctg cgcgggtgtt aatggcgaaa ccgccgcagg ccgttaaccg gggtgatctc
     1021 ggctggttga ccacctcgcc aatggtgatt ctgctggtca tgatgctggc gatgggaacg
     1081 catattccac aacctgtcat caggatcctg gcgggcgctt ccactatagt cctctcaggg
     1141 acgcacgacc tgcctgcaca acgtagcacc tggcatgatt ttttgccttc aggcaccgca
     1201 tctgtttcgg agaaacacag tgaacgttaa ttcatcgtca aatagtggcg aagcgattct
     1261 cgccgccctg aaaacgcagt tccccggcgc ggtactggat gaagagcgac aaacgcctga
     1321 acaggtcacc attacggtga aaatcaatct gctgcctgac gttgtacatt atctttatta
     1381 tcaacatgat ggctggcttc cagtcctgtt tggcaacgac gagcggacac ttaacggtca
     1441 ttacgcggtt tattatgccc tttcaatgga aggggccgaa aaatgctgga tcgtggtgaa
     1501 ggcactggtc gatgccgaca gtcgggagtt tccgtcagtc acaccgcgcg tccctgccgc
     1561 ggtctggggc gagcgagaaa ttcgcgatat gtacgggctg attccggttg gcctgccgga
     1621 tcagcgtcgc ctggtgttgc ccgatgactg gccggaagat atgcatccgc tgcacaaaga
     1681 tgcgatggat tatcgactgc gccctgaacc gacgacagat tccgaaacgt atgcgtttat
     1741 caatgagggc aacagcgatg cgcgggtgat ccctgtcggc ccgctgcata tcacctccga
     1801 tgaaccgggt cacttccgct tgtttgtgga tggcgagcaa attgtcgatg ctgattaccg
     1861 cctgttttat gtccatcgag gcatggagaa actggcagaa acgcggatgg gctacaacga
     1921 agtgaccttc ttatcggacc gcgtgtgtgg gatttgcggt tttgcccaca gtgtggccta
     1981 taccaactcg gttgaaaatg cactggggat tgaggtgccg caacgagcgc ataccattcg
     2041 ctcgattctg ctggaagtcg aacggctaca cagtcatttg ctcaaccttg gcctctcctg
     2101 ccattttgtt ggttttgata ccggctttat gcaatttttc cgcgtgcggg aaaagtcgat
     2161 gacgatggcg gaattgctga ccgggtcgcg taaaacctac ggtctgaatc tgattggtgg
     2221 tgttcgccgc gatattctca aagagcaacg tctgcaaacg ctgaaactgg tgcgcgagat
     2281 gcgcgccgac gtgtcggagc tggtagagat gctgcttgct acgccgaata tggaacaacg
     2341 cactcagggc attggcattc tcgaccgaca aatcgcccgt gattatagcc ctgtagggcc
     2401 gctgatccgc ggcagtggtt ttgcccgtga tttgcgcttt gatcacccct acgccgacta
     2461 cggcaatatt ccaaaaacac tgtttacctt caccggcggc gatgttttct cccgcgtgat
     2521 ggtccgtgtc aaagagacgt ttgattcgct ggcaatgctg gaatttgccc tcgacaacat
     2581 gccggatacc ccactgctga ccgaaggctt tagctataaa cctcacgcat tcgcgctggg
     2641 ctttgttgaa gcgccacgcg gtgaagacgt gcactggagc atgctcggtg ataaccaaaa
     2701 attgttccgc tggcgctgcc gtgccgccac ctacgccaac tggccggtgt tgcgttacat
     2761 gctgcgcggc aataccgttt ctgacgcacc gctgattatc ggtagccttg atccctgcta
     2821 ctcctgtacc gaccgtgtga cgctggtaga tgtgcgcaag cgccagtcaa aaaccgtgcc
     2881 gtataaagag atcgaacgct acggcattga tcgtaaccgt tcgccgctga agtaaggaca
     2941 gaagatgctg aagttactga aaactattat gcgcgccgga accgcgacgg tgaaatatcc
     3001 cttcgcgcca ctggaggtca gcccaggctt tcgcgggaaa ccggacctga tgcccagcca
     3061 atgtattgcc tgcggcgcct gtgcttgtcc ggcaaatgcg ctgactatcc agaccgacga
     3121 ccagcaaaat tcgcgcacct ggcagctcta tctggggcgt tgtatttact gcggacgttg
     3181 tgaagaagtg tgcccgacca gagccatcca gcttaccaat aactttgaac tgaccgtcac
     3241 caataaagcc gatctctata cccgcgcgac gttccatcta caacgttgca gccgttgcga
     3301 acgcccgttt gccccgcaaa aaaccgtcgc actggctgct gaattgttgg cacagcaaca
     3361 aaatgcgcca caaaaccgcg aaatgttgcg ggcgcaagcg agcgtctgcc cggagtgcaa
     3421 acaacgcgcg acgctgatca acgacgatac agatgtaccg ctggtggcta aggagcagct
     3481 atgagtccag tgcttacaca acatgtcagc cagcccatca cgctggatga gcaaacgcaa
     3541 aagatgaagc ggcatttgct acaggatatc cgtcgctcag cctacgttta tcgcgtcgat
     3601 tgcggcggct gcaacgcctg tgaaatcgaa atttttgctg ccatcacccc cgtattcgac
     3661 gcagaacgtt ttggcattaa ggttgtttca tcaccgcgtc acgccgatat tttgttattt
     3721 actggcgcag tcacccgggc gatgcgtatg cctgcacttc gggcgtatga gtctgccccc
     3781 gatcataaaa tctgtgtttc ctacggcgcg tgcggtgtcg gcggcggtat tttccacgat
     3841 ctctacagcg tctggggcgg cagcgacacc attgtcccca ttgatgtttg gatccccggc
     3901 tgcccgccaa caccggccgc caccattcac ggtttcgccg tggcgctcgg tttgctgcaa
     3961 cagaagattc acgctgtgga ttatcgcgat cccaccgggg tgactatgca accgttgtgg
     4021 ccacagatcc cgccatcaca gcgtatcgcc attgagcgag aagcgcggcg gctggcgggc
     4081 tatcgtcagg ggcgagaaat ttgcgatcgg ctcctgcgcc atttaagcga cgatcctacc
     4141 ggaaatcggg ttaacacctg gttgcgcgat gccgacgatc cacgtctcaa tagtatcgtt
     4201 cagcaactct ttcgcgtact ccgggggtta catgactgaa gagtgcgggg aaattgtttt
     4261 ctggacgctg cgaaaaaagt ttgtcgccag tagcgacgag atgccggaac accgctctca
     4321 ggtaatgtat tactcgctgg ctatcggcca tcacgttggc gtgattgatt gtctgaatgt
     4381 cgccttccgc tgcccactga cggaaaacga agattggctt gcacaggtcg aagaggagca
     4441 agcccgacgt aagatgctgg gggtgatgac ctttggtgag attgttattg acgccagcca
     4501 caccgccctg ttgacccggg cattcgcgct actggcagat gacgcgacgt ctgtgtggca
     4561 ggcgcgtagc attcaattca ttcatctgtt ggatgaaatt gtgcaggaac cggccatcta
     4621 tctgatggcc agaaaaattg cgtgagaagg atttctcatt aataaggact gttgatggct
     4681 atgtcagacg aggcgatgtt tgccccgcca caaggaataa caattgaagc ggtaaacgga
     4741 atgctcgcgg agcggttagc acagaaacac ggtaaggcgt ctttattacg cgccttcatc
     4801 ccgatgccgc cgccgttcag cccggtacaa cttattgaac tgcatgttat caaaagcaac
     4861 ttctattacc gctaccatga tgatggcagc gatgtgacgg caacaacaga gtatcagggc
     4921 gagatggtcg attattcgcg tcacgccgtc cttctcggca gtagtggaat ggcggagcta
     4981 cgctttattc gtacccacgg cagccgtttt actccctagg attgcacact gtttaactgg
     5041 ctggcacgca ttatcactcc ggttctgcaa tcatggctca atgatgaaga acaacaggtg
     5101 gcgctgcgtt tgctggagaa agatcgcgat catcatcggg tactggttga tattactaat
     5161 gcagtgctgt cacatcttga tctcgacgat ctgatcgctg acgtcgctcg tgagatccat
     5221 cattttttcg gtctggcttc agtcagtatg gtactgggcg atcatcgaaa gaacgagaag
     5281 tttagcctgt ggtgcagcga tctttctgcc tcacattgtg cgtgtctgcc acgcaatatg
     5341 cctggcgaca gtgtattgct gacacaaacg ctacaaaccc gacaaccgac cttgacgcac
     5401 cgtgcagacg atctgtttct ctggcaacgc gacccgttat tactcttact tgcatctaac
     5461 ggctgcgaat ctgcgctcct tataccgctt acctttggca accatacacc gggtgcattg
     5521 ttgctggcgc atacctcttc cactctcttt agtgaggaaa actgccagct actacaacac
     5581 atagccgatc gcatcgctat tgccgttggc aatgccgatg cctggcgtag catgaccgat
     5641 ttgcaggaaa gtttgcagca agaaaactac cagcttagcg agcagctcct ttcgaatctg
     5701 ggcatcggtg acattatcta tcaaagccag gcaatggaag acctgctcca gcaggtagat
     5761 attgtagcga agagcgacag tacggtgttg atttgtggtg aaaccggaac cggcaaagag
     5821 gtgatcgcca gagcgctcca tcaacttagc acgcgacgcg acaaaccgct ggtcaaaatc
     5881 aactgcgctg ccatccccgc cagtcttctg gaaagtgagt tattcggtca tgacaaaggg
     5941 gcgtttactg gtgcgattaa tacacatcgt ggtcgttttg aaattgccga tggcggcacg
     6001 ttgtttctcg atgaaattgg cgatctgccg ttagaacttc agcctaaact gctgcgcgta
     6061 ttgcaggaac gggagattga gcgtctcggc gggagtagaa cgatcccggt gaatgtcaga
     6121 gtcattgccg ccaccaaccg tgatttgtgg caaatggttg aagatcgcca gtttcgcagc
     6181 aatctctttt atcgcctgaa tgtcttccca ttggaattgc cgccgctacg cgaccgtccg
     6241 gaagatatcc ctcttttagc aaaacatttc acgcaaaaaa tggcgcgcca tatgaatcgc
     6301 gcaattgacg ccatcccgac cgaggcgcta cgccagttga tgtcgtggga ctggccgggc
     6361 aacgtgcacg agctggaaaa cgtgattgag cgagcggtac tgctgactcg cggtaacagt
     6421 ctgaatttac atcttaatgt ccgacaaagc cgtttactgc cgacgctaaa tgaagattca
     6481 gcgcttcgca gttcaatggc gcagttgctg cacccgacga cgccagagaa tgacgaagaa
     6541 gaacgtcagc gcattgttca ggtattgcga gaaaccaatg gcattgttgc cgggccccgt
     6601 ggcgcggcga cacgattagg gatgaagcgc accacgctgc tgtcacgaat gcagcgtctg
     6661 gggatctcgg ttcgcgaggt gttgtaatct gcttttgcag gagtatgcat gagaaacaaa
     6721 ctctctttcg acttgcagtt gagcgccaga aaagcggcaa tcgctgaacg gattgccgtc
     6781 cataaaattg cccgcagtaa agtgtcggtc tttttaatgg cgatgtccgc tggcgtgttt
     6841 atggcgatcg gatttacttt ttacctttcc gttatcgccg atgccccgtc ttcacaggca
     6901 ttaacccatc tggtgggcgg cctttgcttt acactcggct ttattttgct ggcggtttgc
     6961 ggcacagcct gttcacctcg tcggtaatga cggtgatagc aaaaagtcgg ggcgttatta
     7021 gttggcgaac ttggctgatt aacgcacttc tggtggcctg tggtaatctg gcaggtattg
     7081 cctgtttcag tttgttaatc tggttttccg ggctggtgat gagtgaaaac gcgatgtggg
     7141 gagtcgcggt tttacactgc gccgagggca aaatgcatca tacatttact gaatctgtca
     7201 gcctcggcat tatgtgcaat ctgatggttt gcctggcgct gtggatgagt tattgcgggc
     7261 gttcgttatg cgacaaaatc gtcgccatga ttttgcccat caccctgttt gtcgccagtg
     7321 gctttgagca ctgtatcgcc aatttgtttg tgattccgtt cgccattgcc attcgccatt
     7381 tcgccccccc ccttctggca actggcgcac agtagcgcag acaattttcc ggcactgacg
     7441 gtcagccatt ttattaccgc caatctgctc ccggtgatgc tgggtaatat tatcggcggt
     7501 gcagtgctgg tgagtatgtg ttatcgggct atttatttac gtcaggaatc ctgaaaaatc
     7561 agcccggcga aacagttcgt cgggctgaag attattcttg cgcgatttgc ccatcgggcc
     7621 aggcgtggat cacggctttg atcagcgtcg ccaatggaat ggcgaagaat acgccccaga
     7681 atccccacaa accaccgaag atcaccaccg ataaaataat caccagcgga tgcaggttaa
     7741 ccgcttcgga gaacaacacc ggtactagca ggttgccgtc cagcgcctga ataatcagat
     7801 acaccgcgaa gcagctccag aattccgtgc ctgcgccaaa ctggaatagc gccacgccaa
     7861 ccaccggaat ggtcaccaca aatgcgccaa tgtacggaat aagaaccgag aagccgacca
     7921 gcaccgccag cagcagcgaa tagttcagcc caaagagcaa gaaccccagc caggtggcga
     7981 tccccaccac gatcatctcc agcactttgc cgcggatata gttggtgatt tgttgattca
     8041 tctccttcca cacctgtcct gccagtccac ggttgcgcgg cagcacccga cgaacggcgt
     8101 tcagcatctg ctctttgtct ttcagcagga agaagaccat caatggcacc agcaccagat
     8161 agacggcaat ggtcagcaaa ccgaccagcg aggcgaggga aattttcacc accgaatcgc
     8221 ccatggtcag catccgactg cgcatatttt cggccattgc atcaataatg cccgcgtcca
     8281 ttaacgccgg atagcggcgc ggcaacgtgg cggcaaagtc agaaagctta ttgagcatcc
     8341 ccggcatatc gcggattaag tagatgccct gttgccaggc gataggcagt accacgaacg
     8401 ccatcagtag caatataccg acaaaaacca ccaatacaat cgacgtcgcc cagcggcggg
     8461 agcagccaat agattgcagg cgcacggttg gccattccag caaataggcc agcacaatag
     8521 ccaccagcaa cggagcaagc aggccactaa agaaaaagat aatgccaaat ccggcaacta
     8581 aaataaccag caaggcaatc gcttccgggt cgctaaaacg gcggcgatac cattgcatca
     8641 acatttcgag cataaaaacc ttccctgaac ctcaagagcg ggattgcgat ccgcaattgt
     8701 atcgaaatgt cacaaaaaag acttcgcttt ttatgacgga ttcaggaaac tgaaaagtca
     8761 tttgagtggg ctaatcttcg ccgttacact caaaggcggc gcggtgggaa cgatatttca
     8821 cagtatcggt caaatgacta tcttcagaaa tacaggatag aggttatgtt caggcagttg
     8881 aaaaaaaacc tggttgcaac cctcattgct gctatgacca ttggtcaggt agccccggcg
     8941 tttgccgaca acgcagacac cttgccggat atgggaacct ccgcaggaag cacgctttcc
     9001 attggtcagg aaatgcagat gggcgactat tatgtccgcc agctacgcgg cagcgcgccg
     9061 ttaattaatg acccgctgtt aacgcaatat attaattcgc tggggatgcg tctggtttcg
     9121 catgccaatt cggttaagac accgtttcat ttttttctaa tcaacaacga cgaaattaac
     9181 gcctttgctt tctttggcgg caacgtggtg ctgcactctg ccctgttccg ttattccgat
     9241 aacgaaagtc aactggcttc agttatggcg cacgaaatct cccacctcac ccaacgtcac
     9301 ctggcgcgag cgatggaaga tcagcagcgc agcacgccgc tgacctgggt cggcgcgtta
     9361 ggttctattt tactggcgat ggccagtccg caggcgggga tggcggcgct gaccggtaca
     9421 ctggcgggaa cgcgtcaggg gatgatcagt ttcacccagc aaaatgaaca ggaagcggac
     9481 cgcattggta ttcaggtgct gcaacgctcg ggattcgatc cgcaggcgat gccaaccttc
     9541 ctcgaaaaat tactcgatca ggcgcgttac tcctcgcgcc cgccggaaat tttattgact
     9601 cacccgttgc cggaaagtcg tctggcagat gcccgcaacc gtgctaatca gatgcgaccg
     9661 atggtggtgc agtcgtcgga agatttctat ctggcgaaag cgcgcacact ggggatgtat
     9721 aattccggac gtaaccagct caccagtgat ttgctggatg aatgggcgaa aggaaacgtt
     9781 cgtcagcaac gagcggcgca atatggtcgt gctttacagg cgatggaagc caataaatac
     9841 gacgaggcgc gtaaaacgct gcaaccgtta ctggcggcag aacctggcaa cgcatggtat
     9901 ctcgatctgg ctaccgatat cgatcttggg caaaacaaag ccaatgaggc aatcaatcgc
     9961 ctgaaaaatg cccgtgattt gcgcaccaat ccggtgttgc agctcaacct ggcaaacgct
    10021 tatctgcaag gcggtcaacc acaagaagcg gccaatattc tcaatcgcta cacctttaat
    10081 aataaagatg acagcaacgg ctgggattta ctggcacagg cggaagccgc gctaaataac
    10141 cgcgatcagg agctggctgc gcgagcagaa ggttatgcgc tcgccggacg actcgatcag
    10201 gccatttcgc tgttgagtag cgccagttcg caggtgaaat taggcagcct gcaacaagcg
    10261 cgttacgatg cgcgcatcga ccagttgcgc cagctgcagg aacgctttaa gccttatacc
    10321 aagatgtaat tcgcattaaa ggaagaacaa tgaccaaaca ggtaaaaatc taccataacc
    10381 cacgctgttc aaagagccgg gaaacgctga acctgctgaa agaaaacggc gtggagccag
    10441 aagtggtact ctaccttgag acacccgccg atgcggcaac gctgcgcgat ttgctgaaaa
    10501 tgctggggat gaatagcgcc cgtgaattga tgcgccagaa agaggatctt tataaagaac
    10561 tgaacctggc tgacagttcg cttagcgaag aggcgctgat tcaggcaatg gtggataatc
    10621 cgaagctgat ggagcgcccg attgtggtcg cgaatggcaa agcgcggatt ggtcgtccac
    10681 cggagcaggt actggaaatc gtgggttaag atgtgctcca tcgctagttg aagcacattg
    10741 ccggatgcga cgcttgccat gtcttatccg gcctacgaat actatggatt aacttcgcgt
    10801 agttcggata aggcgttcgc gccgcatccg acaataaaca ccttatctac aacttcagaa
    10861 tttctttcac aaacggaatg gtcagcttac gttgcgcggt aatcgacgca cgatccaact
    10921 gatccaacgt cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc
    10981 ccacatcttc cggcagttca aaaccacgca aacgcgcgcg taactgtagc gcctgcaact
    11041 tatcttcatc agaaagtggc tgcaatttgt agatctgccc ccagtcgagt cgcgacgcga
    11101 gatccggtaa tcccagattc aactgccgcg gtggacgatc gccggtgatc aacaaccgtg
    11161 ttttgcccga ttccagaatt cgattgtaga gatcgaaaat cgccatctcc cacaactcat
    11221 cgcctgcaat acactcaatg ttgtcgatac aaaccagcga caaatgctcc ataccgtcga
    11281 gcacttccgg aacaaaccaa gtgcgtttat ccagcgggac atagcccacc gcatcgccac
    11341 gctgcgacaa ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg
    11401 cccagagata gatgtaaccg ctatgttcct gacgcagcac gttttgcagc gcggccagta
    11461 aagaggagtt atcccccggc cagaaacttg caaaggtttc gtcgtcagga agataaagtg
    11521 gcaaagagag ctgtgccggt gtgttcagag atacctcaac caggatttca caaaatcgcg
    11581 agaagtttac cacagaatcc catgatgttt gaaccgggca gcaacactgc ccggtcggta
    11641 cattatttgt ctgttatatc cgcctcttct gcgtccagca ccacttcttc agggcgcagc
    11701 acactgatca gtttgaaaat caaacttaag ccgataccga cgatggtcgc cagcgccata
    11761 ccttttaact ccgccgcgcc gatgtttacc ttcgcgccac tgacgccgat gatcaaaatc
    11821 acggaagtca ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc gatcaaaaca
    11881 cgaataccgg aagcaccgat gacaccataa agcagcagcg aaacgccgcc catcaccggc
    11941 aatgggatca tctggatagc ggcagccagt ttaccgacgc aggaaagcag gatagcgaaa
    12001 atcgccgccc cgccgataac ccaggtactg taaacacggg tgatcgccat cacgccaatg
    12061 ttttctccgt aagtggtatt tggcgtggag ccaaagaagc cggagatcac ggtcgacagg
    12121 ccgttagcaa acatcgaacg gtgcagacct ggatcgcgca gcagatcttt tttgacgata
    12181 ttagccgtta ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc cgctggcaga
    12241 atagtcagaa tggcaaacca ctcgaagcgc ggcgtataga gggttggcag cgcaaaccag
    12301 tgagcattaa taatcggcgt ggtatcgaca attcccattg cgaaagagag cgcgtagccc
    12361 accagcacgc caattaaaat cgggataatt gccaggaaac cacgaaacag cacggaacct
    12421 aaaaccgtga ccgccagggt ggtaatagag atgatgatgg ttttggagtc tggcgtttgc
    12481 ccttcagccg ggagtaaacc cgccataccg gcagctacgc ccgccagctc cagaccgatg
    12541 acggcaacga ttgcgcccat tgccgcaggt ggaaacagca cgtccagcca gccggtcccc
    12601 gctttcttca cgataaaaga aaccaggcag aacagcacgc cgcacataat aaagccgccc
    12661 agcgcgactt cataccctaa cggcaacagt aacaataccg gtgaaataaa ggcaaagctg
    12721 gaaccaagat aagccggaat tttcccttta cagatgaaga gatacagcag cgttccaata
    12781 ccgttaaata acagtacagt cgccgggtta atatgaaata agacgggcac caggacggtt
    12841 gcaccaaaca tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt
    12901 ctttcactca ccccgatagc acggcgcgtc atagtattat cctctgtatt atgtgttata
    12961 ggcgctttac tcaaaaaaaa gccgactctt aaagtcggct ttaattattt ttattcttta
    13021 tttcgtacca aagattttgt caccggcatc gccgaggccc ggaataatgt atccgtgctc
    13081 gttcagtccc tgatcaatcg atgcggtata cagttcgacg tccgggtgcg ctttttccag
    13141 cgcagcgata ccttctggcg cagcgaccag caccagaact ttgatgctgc tgcagcccgc
    13201 ttttttcagc aggtcgatgg tcgcgataac ggaaccaccg gttgccagca ttgggtcaac
    13261 gatcagcgcc atacgctcat cgatgttaga aaccagtttc tggaagtacg gtaccggctc
    13321 cagcgtttct tcattacggt acataccgac aacgctgatg cgcgcgctcg gaacattttc
    13381 cagcacaccg tccatcatac caagacccgc acgcagaatt ggcacaacgg taattttctt
    13441 acctttgatc tggtcgattt ctaccgggcc gttccagcct tcgatagtta ctttttccgt
    13501 ttcgaggtcg gcggtcgctt cgtaagtcag caggctaccc acttcggaag cgagttcgcg
    13561 aaagcgcttg gtgctgatat cttgctcacg catcagtccc agcttgtgtt tgacgagtgg
    13621 gtgtttgact tccacgatct tcatactctt tctcctttga ggggcagcca caaaaaaatc
    13681 gacggattat acctcctttc ttcaaggcgg caatattctt ttcgttgact ttagtcaaaa
    13741 tgataacgat ttgagataaa gttattttat attcaaatgg ttatgaaaga agatcattcc
    13801 atccgaaaac taacctttgc cctggcacaa gtcttctttc gccgcgcgtc tggggaaaag
    13861 acgtgcaaaa aggttgtgca aagcagcctc gcaaacgttt gctttccctg ttagaattgc
    13921 gccgaatttt atttttctac cgcaagtaac gcgtggggac ccaagcagtg accgataaaa
    13981 cctctcttag ctacaaagat gccggtgttg atattgacgc gggtaatgct ctggttggaa
    14041 gaatcaaagg cgtagtgaag aaaacgcgtc gtccggaagt gatgggcggt ctgggcggct
    14101 tcggtgcgct gtgtgcattg ccgcaaaaat atcgtgaacc cgtgctggtt tccggcactg
    14161 acggcgtagg taccaagctg cgtctggcaa tggacttaaa acgtcacgac accattggta
    14221 ttgatctggt cgccatgtgc gttaatgacc tggtggtgca aggtgcagaa ccgctgtttt
    14281 tcctcgacta ttacgcaacc ggaaaactgg atgttgatac cgcttcagcg gtgatcagcg
    14341 gcatcgcgga aggttgtctg caatcgggct gttcactggt gggtggcgaa acggcagaaa
    14401 tgccggggat gtatcacggt gaagattacg atgtcgcggg tttctgcgtg ggcgtggtag
    14461 aaaaatcaga aatcatcgac ggctctaaag tcagcgacgg cgatgttctg attgcactcg
    14521 gttccagcgg tccgcactcg aacggctatt cgctggtgcg caaaattctt gaagtcagcg
    14581 gttgtgatcc gcaaaccacc gaacttgatg gtaagccatt agccgatcat ctgctggcac
    14641 cgacccgcat ttacgtgaag tcagtgctgg agttgattga aaaggtcgat gtgcatgcca
    14701 ttgcgcacct gaccggcggc ggcttctggg aaaacattcc acgcgtattg ccagataata
    14761 ctcaggcagt gattgatgaa tcttcctggc agtggccgga agtgttcaac tggctgcaaa
    14821 cggcaggtaa cgttgagcgc catgaaatgt atcgcacctt caactgcggc gtcgggatga
    14881 ttattgccct gcctgctccg gaagtggaca aagccctcgc cctgctcaat gccaacggtg
    14941 aaaacgcgtg gaaaatcggt atcatcaaag cctctgattc cgaacaacgc gtggttatcg
    15001 aataatgaat attgtggtgc ttatttccgg taacggaagt aatttacagg caattattga
    15061 cgcctgtaaa accaacaaaa ttaaaggcac cgtacgggca gttttcagca ataaggccga
    15121 cgcgttcggc cttgaacgcg cccgccaggc gggtattgca acgcatacgc tcatcgccag
    15181 cgcgtttgac agtcgtgaag cctatgaccg ggagttgatt catgaaatcg acatgtacgc
    15241 acccgatgtg gtcgtgctgg ctggttttat gcgcattctc agcccggcgt ttgtctccca
    15301 ctatgccggg cgtttgctga acattcaccc ttctctgctg ccgaaatatc ccggattaca
    15361 cacccatcgt caggcgctgg aaaatggcga tgaagagcac ggtacatcgg tgcatttcgt
    15421 caccgatgaa ctggacggtg gcccggttat tttacaggcg aaagtcccgg tatttgctgg
    15481 tgatacggaa gatgacgtca ccgcccgcgt gcaaacccag gaacacgcta tttatccact
    15541 ggtgattagc tggtttgccg atggtcgtct gaaaatacac gaaaacgccg cgtggctgga
    15601 tggtcaacgt ctgccgccgc agggctacgc tgccgacgag taatgccccc gtagttaaag
    15661 cgccagctct gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt
    15721 tttccccctt ttctggcata gttggacatc tgccaatatt gctcgccata atatccaggc
    15781 agtgtcccgt gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc tatacatcga
    15841 aaaagagctc agttggttat cgttcaatga acgcgtgctt caggaagcgg cggacaaatc
    15901 taacccgctg attgaaagga tgcgtttcct ggggatctat tccaataacc ttgatgagtt
    15961 ctataaagtc cgcttcgctg aactgaagcg acgcatcatt attagcgaag aacaaggctc
    16021 caactctcat tcccgccatt tactgggcaa aattcagtcc cgggtgctga aagccgatca
    16081 ggaattcgac ggcctctaca acgagctatt gctggagatg gcgcgcaacc agatcttcct
    16141 gattaatgaa cgccagctct ccgtcaatca acaaaactgg cttcgtcatt attttaagca
    16201 gtatctgcgt cagcacatta cgccgatttt aatcaatcct gacactgact tagtgcagtt
    16261 cctgaaggat gattacacct atctggcggt ggaaattatc cgtggagaca ccatccgtta
    16321 cgcgctgctg gagatcccat cagataaagt gccgcgcttt gtgaatttac cgccagaagc
    16381 gccgcgtcga cgcaagccga tgattcttct ggataacatt ctgcgttact gccttgatga
    16441 tattttcaaa ggcttctttg attatgacgc gctgaatgcc tattcaatga agatgacccg
    16501 cgatgccgaa tacgatttag tgcatgagat ggaagccagc ctgatggagt tgatgtcttc
    16561 cagtctcaag cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc
    16621 caatgcgctg gttgaagtgt tacgcgaaaa actgactatt tcccgctacg actccatcgt
    16681 ccccggcggt cgttatcata attttaaaga ctttattaat ttccccaatg tcggcaaagc
    16741 caatctggtg aacaaaccac tgccgcgttt acgccacatt tggtttgata aagcccagtt
    16801 ccgcaatggt tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc cttatcacac
    16861 ctttgagcat gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg tactggcgat
    16921 taaaattaac atttaccgcg tggcgaaaga ttcacgcatc atcgactcga tgatccacgc
    16981 cgcacataac ggtaagaaag tcactgtggt ggttgagtta caggcgcgtt tcgacgaaga
    17041 agccaacatt cactgggcga agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc
    17101 gccggggctg aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt
    17161 ggtccgttac gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac
    17221 tgactattcg ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc gggtatttaa
    17281 ctttattgaa aacccatacc gcccagtgac atttgattat ttaatggtgt caccgcaaaa
    17341 ctctcgccgt ctgttatatg aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct
    17401 gcccagtggt atcaccctga agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct
    17461 gtatgcggcc tccagctccg gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct
    17521 gatccccaat ccggaaggca ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta
    17581 ccttgaacat gaccgggttt atatttttga aaatggcggc gataaaaagg tctacctttc
    17641 ttccgccgac tggatgacgc gcaatattga ttatcgtatt gaagtggcga caccgctgct
    17701 cgatccgcac ctgaagcagc gggtgctgga catcatcgac atattgttca gcgatacggt
    17761 caaagcacgt tatatcgata aagaactcag taatcgctac gttccccgcg gcaatcgccg
    17821 caaagtacgg gcgcagttgg cgatttacga ctacatcaaa tcactcgaac aacctgaata
    17881 accctatgcc aatacacgat aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg
    17941 gttcaaacag ttttcacatg gtcatagccc gtgtggtaga tggtgccatg cagattattg
    18001 gccgcctgaa acagcgagtg catctggcgg acggcctggg gccagataat atgttgagtg
    18061 aagaggcaat gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt
    18121 tttctcctgc cagcgtctgt atagtcggca cccatacgct gcgtcaggcg ctgaacgcca
    18181 ctgactttct gaaacgcgtg gaaaaggtca ttccctaccc gattgaaatt atttccggta
    18241 atgaagaagc ccgtctgatt tttatgggcg tggaacatac ccaaccggag aaaggtcgca
    18301 aactggttat tgatattggc ggcggatcta cggaactggt gattggtgaa aatttcgaac
    18361 cgattctcgt tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt tacttccctg
    18421 gcggggtcat caataaagag aattttcagc gcgctcgtat ggcggcagca caaaaactgg
    18481 aaactttaac ctggcaattc cgtattcagg gctggaacgt ggcaatgggc gcttccggta
    18541 ccataaaagc cgcccatgaa gtgttaatgg aaatgggcga gaaagacggg ataattaccc
    18601 cggaacgtct ggaaaaactg gtaaaagaag ttttacgtca ccgtaatttc gcatcgctga
    18661 gtttaccggg tctttccgaa gagcggaaaa cagtcttcgt tcctggactg gcgattttat
    18721 gcggtgtgtt tgatgcttta gccatccgtg aactgcgcct ttctgacggg gcgttacgcg
    18781 aaggcgtact gtacgaaatg gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg
    18841 ccagcagcct cgccaaccag tatcacatcg acagcgaaca ggcccgacgg gtgctggata
    18901 ccactatgca aatgtacgaa cagtggcggg aacagcaacc gaagctggcg catccgcaac
    18961 tggaggcgct actgcgatgg gccgccatgc tgcatgaggt cgggttgaat atcaaccaca
    19021 gcggtttgca tcgccactcc gcttatattc tgcaaaacag tgacttgccg ggttttaatc
    19081 aggaacagca gctgatgatg gcgacactgg tgcgctatca ccgcaaagcg attaaactcg
    19141 acgatctgcc gcgctttacc ttgtttaaga agaaacagtt cctgccactg attcagctat
    19201 tgcgccttgg cgtattactc aacaatcaac gtcaggcaac caccacaccg ccaacattga
    19261 cactgattac tgatgacagt cactggacac tgcgtttccc gcatgactgg tttagtcaga
    19321 atgcgttggt actgcttgat ctggaaaagg agcaagaata ctggga
//
LOCUS       DAJTOR010000070        19153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTOR010000070 DAJTOR010000000
VERSION     DAJTOR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.070"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..909)
                     /locus_tag="OO249_002151"
     CDS             complement(<1..909)
                     /locus_tag="OO249_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR5636445.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQAL"
     gene            1376..2608
                     /gene="mdtM"
                     /locus_tag="OO249_002152"
     CDS             1376..2608
                     /gene="mdtM"
                     /locus_tag="OO249_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCR5636446.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            2649..3929
                     /locus_tag="OO249_002153"
     CDS             2649..3929
                     /locus_tag="OO249_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCR5636447.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            4045..5197
                     /locus_tag="OO249_002154"
                     /pseudo
     CDS             4045..5197
                     /locus_tag="OO249_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
     gene            5207..5975
                     /locus_tag="OO249_002155"
                     /pseudo
     CDS             5207..5975
                     /locus_tag="OO249_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5972..6229
                     /locus_tag="OO249_002156"
     CDS             5972..6229
                     /locus_tag="OO249_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCR5636448.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            6183..7154
                     /locus_tag="OO249_002157"
     CDS             6183..7154
                     /locus_tag="OO249_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCR5636449.1"
                     /translation="MVSGAALHITGFLRVKDSLRSLRILLDILLICLKGITVTKSISL
                     SKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNYHATIDGKEIAGITNNISSLTW
                     SAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKDLETIEYIGDNQFVISDERDYA
                     IYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYSRQDHTFWFFKEKNPIEVYKVN
                     GLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIG
                     EISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNRFYRFTPKSSH"
     gene            7222..8394
                     /locus_tag="OO249_002158"
     CDS             7222..8394
                     /locus_tag="OO249_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5636450.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVIMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALARGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIALGNEYVLAGL
                     HFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAILYG
                     LAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPCLT
                     ANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLLGF
                     VTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS"
     gene            complement(8407..8961)
                     /gene="kptA"
                     /locus_tag="OO249_002159"
     CDS             complement(8407..8961)
                     /gene="kptA"
                     /locus_tag="OO249_002159"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5636451.1"
                     /translation="MAKHNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            9211..9894
                     /locus_tag="OO249_002160"
     CDS             9211..9894
                     /locus_tag="OO249_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636452.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9891..10352
                     /locus_tag="OO249_002161"
     CDS             9891..10352
                     /locus_tag="OO249_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5636453.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10365..11537
                     /gene="iadA"
                     /locus_tag="OO249_002162"
     CDS             10365..11537
                     /gene="iadA"
                     /locus_tag="OO249_002162"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5636454.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11602..12513
                     /gene="hypT"
                     /locus_tag="OO249_002163"
                     /gene_synonym="qseD"
     CDS             11602..12513
                     /gene="hypT"
                     /locus_tag="OO249_002163"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5636455.1"
                     /translation="MDDCCAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEYGEAIFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12506..12898)
                     /gene="iraD"
                     /locus_tag="OO249_002164"
     CDS             complement(12506..12898)
                     /gene="iraD"
                     /locus_tag="OO249_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5636456.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12895..12978)
                     /gene="idlP"
                     /locus_tag="OO249_002165"
     CDS             complement(12895..12978)
                     /gene="idlP"
                     /locus_tag="OO249_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5636457.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            13570..14400
                     /locus_tag="OO249_002166"
     CDS             13570..14400
                     /locus_tag="OO249_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5636458.1"
                     /translation="MSIPLSNALQSQIITDNHFLHHPKVDNKLTREYEYTRMDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLNIGKQSWPFNYATDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGYNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(14541..15314)
                     /gene="uxuR"
                     /locus_tag="OO249_002167"
     CDS             complement(14541..15314)
                     /gene="uxuR"
                     /locus_tag="OO249_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5636459.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(15529..16989)
                     /locus_tag="OO249_002168"
     CDS             complement(15529..16989)
                     /locus_tag="OO249_002168"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5636460.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLKAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(17070..18254)
                     /gene="uxuA"
                     /locus_tag="OO249_002169"
     CDS             complement(17070..18254)
                     /gene="uxuA"
                     /locus_tag="OO249_002169"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5636461.1"
                     /translation="MEQTWRWYGPNDLVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            18594..18798
                     /locus_tag="OO249_002170"
                     /pseudo
     CDS             18594..18798
                     /locus_tag="OO249_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19041..>19153)
                     /locus_tag="OO249_002171"
     CDS             complement(19041..>19153)
                     /locus_tag="OO249_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5636462.1"
                     /translation="GTSAVSLGLTANYARTGGQVTAGNVQSIIGVTFVYQ"
BASE COUNT         4474 a         4872 c         4808 g         4999 t
ORIGIN      
        1 taatgcctgc atttgctctg ccgaaagtcc ggtatatcgt tgtatccatt caggatcggc
       61 cccattctgc aacaacaatc ggaggaggct ccgttcccct tctactttcc cttctttcct
      121 ccccctctcc cttcccaaca gccatccatc attatgaatt cgctctgcaa ttgtcattat
      181 tcgctccctg tggtgtggca ttcgcctggt aagttcgctg ataaacgcct taaaacgcgc
      241 ttcatcgcca gtcagtaaaa tgtaatttaa cagcgcagtt atctggctgt cattagcaca
      301 ctctgtaacc agtaatacta ccagttggtc aataagcccc atcaaatcgc gctggcgaat
      361 atgcttttgg atcaactcca acagggcgac tctgcgatgc tgcacaatct cgtcgtctgg
      421 cacgacagtg acatccacca gcgggaacgc tgcgctataa agtttccgtg cggtagtcgg
      481 ggcggcaaat tcgtccagcc agcacaggga ccaggggtaa ggactacggc taccgtgata
      541 aaacagcatc gggataacca gcggtagcgg ccggcgttta tcatgctcga tatggcgctg
      601 catcaccgcc atggaatatc gcatcaggcg aaatgccata tgaatatcct cgcggctctg
      661 atgttcaatc actacgtaaa tataaccatc accttcacgg gtctttaccg accacagaat
      721 atcggagtgt agcgcccgca atttttcatc gacaaagctg gcggattcca gttttaagct
      781 gtcgagatcg cacagttcac gtaaatcttt gggtaagtgg atctccataa aatcccgcgc
      841 ggtgtcaggg tgagtgagaa aggatttaaa taatgcgtca tgtggcgtgc tggtcgtgaa
      901 gtttgtcatg gcgatccgtc acctgtagat aagatggccc gactttagcg actaacaaaa
      961 ccattctcac ccggcaattt ctcgttcccc gaggtgcttt ccaggcgtct gcaaacgata
     1021 catttttccg gaaacaggct cgcgttttac atttcctgta cccggcagat attccccttt
     1081 ccaccgcgct cttttgatta accgcaaatt ttaattacac ttaaggtgta tattttctat
     1141 gcaacccatc aattaaagag gtgtaatgtg ctgatgacta tttgtaatcg ttatacattc
     1201 tgacctggag tcagaaggta tttctctgtc tgtgtgttca caggcagtgt ggttgattac
     1261 atgaaattca gtacatttgc agtctcgttg cccttctcgc ctgcctttcg tcattaccga
     1321 cggtattgaa tttcgttttc cccgttgggg ttctccgaac aaggagttgt ttgttatgcc
     1381 acgttttttt gcccgccatg ccgccacgct gtttttcccg atggcgttga ttttgtatga
     1441 ctttgccgcg tacctgtcga cggatctgat ccagccgggg atcattaatg tggtccgtga
     1501 ttttaatgcc gatgtcagtc tggccccggc agccgtcagt ctctatctcg cgggcggtat
     1561 ggcgttacag tggctgctgg ggccgctttc cgacaggatt ggccgcaggc cggtgctgat
     1621 taccggggcg ctaatattta cccttgcctg cgctgcgaca atgttcacaa cgtctatgac
     1681 acagtttctt atcgcgcgtg caattcaggg caccagtatc tgttttattg ccaccgttgg
     1741 ttatgtcacg gtgcaggagg cgttcggcca gacaaaaggg atcaagttga tggcgattat
     1801 cacctccatc gtactgattg cgccgattat tggcccgctt tccggcgcag ctctgatgca
     1861 ctttgtgcac tggaaagtcc tttttgccat cattgcagtt atgggtttta tctcatttgt
     1921 tggcttactg ctggcgatgc cagagacggt gaagcgcggc gcggttccat ttagcgccaa
     1981 aagcgtcttt cgcgattttc gtaatgtctt ttgcaatcgg ctgttcctct ttggcgcagc
     2041 aaccatctct ttaagctata tcccaatgat gagttgggtg gctgtctcgc cggtgatcct
     2101 gatcgacgcg ggcagcttaa caacttcgca gttcgcctgg acacaggttc cggtgttcgg
     2161 cgcggtgatt gtcgcgaatg ccatcgtggc gcgtttcgtt aaagatccga ccgaaccgcg
     2221 ctttatctgg cgtgctgtac ccattcaact ggtcggcctc gcgctgttga ttatcggcaa
     2281 tctgctgtca ccgcacgtct ggctgtggtc ggtgctgggc accagtttgt atgctttcgg
     2341 gattggtttg atcttcccga ccttattccg ctttacgctt ttttccaata acttaccgaa
     2401 agggaccgtc tccgcatcgc tgaatatggt gatcctgatg gtgatgtctg tctcggtcga
     2461 aatcggccgc tggctgtggt ttaacggcgg tcgcttgccg tttcatctgt tagccgtcgt
     2521 ggcgggcgtt atcgtcgttt tcaccctggc aggattgctt aatcgcgtgc gccaacatca
     2581 ggctgccgag ctggcggagg agcggtgatt tttgcgcgat cctgccgtca ggctctattc
     2641 ttaacgttat gaataaactc attgaactca gacgcgccaa aatgttggcg ctctctttac
     2701 tgcttatcgc cgctgctacc tttgtcgtta cgctgttttt gccgcccaat ttttgggtga
     2761 gcggcgtgaa ggcgattgct gaagcggcga tggtcggcgc gctggcggac tggtttgcgg
     2821 tggtggcgct gtttcgccgc gtgccgattc cgatcatttc tcgtcatacg gcgattatcc
     2881 cgcgtaataa agaccggatt ggcgaaaatc tcggtcagtt cgtgcaggaa aaatttctcg
     2941 atacccagtc gctggtggca ttgattcgac gtcacgaacc ggcgttgttg attggcaact
     3001 ggtttagtca gccggagaac gcccgccgcg ttggtcagca tctgttgcag atcatgagcg
     3061 gttttcttga actgaccgat gatgcgcgta ttcagcgcct gcttaagcgc gcggtccatc
     3121 gggcgattga taaggtcgat ctttccggaa ccagtgcgtt gatgctggag agtatgacca
     3181 aaaacgatcg tcatcaggtg ctgctcgata cgctgatcgc acagttgatc gcccttctcc
     3241 agcgcgataa atcgcgcaag tttattgccc agcaaattgt tcgctggctg gagagcgagc
     3301 atccgctgaa agccaaaatt ttgcctactg aatggctggg cgaacatagc gcggagttgg
     3361 tttctgacgc ggtgaattct ttgcttgatg atatcagccg cgatcgtgcg catcagatcc
     3421 gtcatgcgtt tgatcgcgcc acttttgccc tgatcgacaa gctgaaaaac gatccagaaa
     3481 tggcagcgcg agccgatgcc gtaaaaagtt atctgaaaga agatgaagct tttaaccgct
     3541 atctcagtga attgtggggg gatttacggg aatggctgaa agcggatatc aacagtgaag
     3601 attctcgtgt gaaagaacgt atcgcgcggg cgggtcaatg gtttggcgaa acgttaattg
     3661 ccgatgatgc cttgcgggca tcgttaaatg gtcacctgga acaagccgcg caccgcgtcg
     3721 cgcctgagtt ttccgcattc ctgacgcgcc acatcagcga tacagtaaaa agctgggatg
     3781 ctcgggatat gtcgcggcaa atcgagttaa atatcggcaa agatctgcag tttatccgtg
     3841 tcaacggtac gctggttggc ggttgtattg ggctaatttt atatttgttg tcgcagctcc
     3901 cggccttgtt ccccctcagc aatttttaga aatccatgaa aaactaagtg gctaaccgcg
     3961 agagagatca aataaaccac actaaaagaa tggtaataat gtggcctctt ttcattatct
     4021 cccgtggtac ggggaaggaa aatcatgtca cttatcaccg atctacccgc cattttcgat
     4081 cagttctctg aagctcgcca gaaaggcttt ctcaccgtca tggatctcaa ggagcgcggc
     4141 attccgctgg ttggcactta ctgcaccttt atgccgcaag agatcccgat ggcagccggt
     4201 gcggttgtgg tttcgctctg ttctacctct gatgaaacca ttgaagaagc ggagaaagat
     4261 ctgccgcgca acctctgccc gctgattaaa agcagctacg gcttcggcaa aaccgataaa
     4321 tgcccctact tctacttttc ggatctggtg gtcggtgaaa ccacctgcga cggcaaaaag
     4381 aaaatgtatg aatacatggc ggagtttaag cccgttcatg tgatgcagtt gccgaacagc
     4441 gttaaggacg atgcctcgcg tgcgttatgg aaagccgaga tgctgcgctt gcaaaaaacg
     4501 gtagaagaac gttttgggca cgagattagc gaagatgctc tgcgcgatgc cattgcgctg
     4561 aaaaaccgcg aacgtcgcgc actggccaat ttttatcatc ttgggcagtt aaatcctccg
     4621 gcgcttagcg gcagcgacat tctgaaagtg gtctacggcg caaccttccg gttcgataaa
     4681 gaggcgttga tcaatgaact ggatgcaatg accgcccgcg ttcgtcagca gtgggaagaa
     4741 ggccagcgac tggacccgcg tccacgcatt ttaatcaccg gctgcccgat tggcggcgca
     4801 gcagaaaaag tggtgcgggc gattgaagag aatggcggct gggttgtcgg ttatgaaaac
     4861 tgcactgggg cgaaagcgac cgagctaatg cgtggcggaa acgggcgatg tctacgacgc
     4921 gctggcggat aaatatctgg cgattggctg ctcctgtgtt tcgccgaacg atcagcgcct
     4981 gcaaatgctc agccagatgg tggaagaata tcaggtcgat ggcgtagttg atgtgatttt
     5041 gcaggcgtgc catacctacg cggtggaatc gctggcgatt aaacgtcatg tgcgtcagca
     5101 gcacaacatt ccttatatcg ctattgaaac agactactcc acctcagatg tcgggcagct
     5161 cagtacccgt gtcgcggcct ttattgagat gctgtaagga gtggcagtga catattcgat
     5221 tggcattgat tctggttcta ccgccaccaa agggatctta ctggcagacg gcgtgattac
     5281 gcgccgtttc ctcgttccaa caccctttcg tccggcaaca gcaattactg aagcctggga
     5341 aactctgcgc gaagggttag agacaacgcc gtttctgacg ctcaccggct atggacggca
     5401 actggtggat tttgccgata aacaagtgac ggaaatctcc tgtcacgggc tgggcgcacg
     5461 gtttcttgcg ccagcaacgc tgcgcggtaa tcgacatcgg tggtcaggac agcaaagtga
     5521 ttcagcttga tgatgacggt aacctgtgcg atttcctgat gaatgacaaa tgcgcggcgg
     5581 gcaccgggcg tttcctggaa gtgatctcgc gcacgcttgg caccagcgtc gagcaactcg
     5641 acagcattac cgaaaatgtc acgccgcatg ccatcacgag tatgtgcaca gtgtttgccg
     5701 aatcagaagt gatcagcctg cgctcagcgg gcgtcgcgcc agaagcgatt ctcgccggag
     5761 tgattaacgc gatggcgcgg aggagtgcca atttcattgc tcgtctctcc tgtgaagcgc
     5821 cgattctgtt tactggtggc gttagtcatt gccagacgtt tgcccggatg ctggaatctc
     5881 acctgggaat gccggtaaat acccatcctg atgcgcaatt tgctggcgca attggcgcgg
     5941 cggtaattgg tcaacgagtg aggacacgcc gatgaaagag tttttatttt tatttcactc
     6001 cacagtcggc gtcatacaaa ctcgcaaagc gttgcaggca gcgggcatga cttttcgcgt
     6061 cagcgatatt ccccgtgatt tacgcggcgg ctgcgggtta tgcatttggc tgacctgtcc
     6121 ccccggcgaa gaaatacaat gggtgatccc tgggctgact gagtcaattt attgccagca
     6181 ggatggtgtc tggcgctgca ttgcacatta ccgggtttct ccgcgttaag gattcattaa
     6241 ggtcgctacg tatacttctc gatatcttat tgatttgcct taaagggatt accgtgacaa
     6301 aaagtatttc gttaagtaaa cgcatctccg ttattgtgat tctttttgcc attgtggcag
     6361 tgtgtacttt cttcgtgcaa tcttgcgccc gtaaaagcaa tcatgcagca tcatttcaga
     6421 attaccacgc caccattgac ggcaaagaaa ttgcaggcat aacgaataat atctcttcgc
     6481 tgacctggtc ggcacaaagc aatactcttt ttagcactat caacaaacct gccgccattg
     6541 ttgaaatgac caccaatggt gattttatcc gcacaattcc tctggatttt gttaaggatc
     6601 tcgagacgat cgaatacatt ggcgataatc agtttgtcat tagcgatgag cgcgattacg
     6661 ccatttatgt gatttcactg accccaaact cggaagtaaa aatcctcaaa aaaataaaaa
     6721 tcccgctgca agactcccca accaattgcg gttttgaagg gttagcttat tctcgccagg
     6781 atcacacttt ctggtttttt aaagagaaaa atccgattga ggtctacaaa gttaacgggc
     6841 tgttaagtag caatgaatta cacatcagca aagacgaggc actacaacgg caatttactt
     6901 tagatgatgt gtccggcgca gaatttaacc agcaaaaaaa tacgcttctg gtgctgtctc
     6961 atgaatctcg cgcactccag gaagtaaccc tggttggcga ggttatcggt gaaatatcac
     7021 taaccaaagg tagtcgaggt ttgtcgcata acatcaaaca ggccgaaggg attgcgatgg
     7081 atgcctctgg taatatttat atcgttagcg aaccaaatcg tttttatcgc tttaccccta
     7141 aatcatcgca ttaacaggct ggcatatttc acctttttgt tattaacttg ttaacaacaa
     7201 aaatgacata caaggttaaa catgccttcg tcctcgcatc ccgtagaacg cttttctttc
     7261 agcaccgcgc ttttcgggat gctggttctg accctaggta tgggattagg tcgcttcctt
     7321 tatacgccta tgttgcccgt cattatggcg gaaggggcgt tttcatttag ccagctctcg
     7381 tggattgcca gcggcaacta tgcggggtat ctggctggca gtctgctatt ttcgtttggc
     7441 gcatttcacc agccgtcgcg cctacgccca tttctgttgg tttctgccct ggcgagaggg
     7501 ttgttgatcc tcgcaatggc atggttgccg ccatttattc tggtgttatt gattcgcttc
     7561 ctggcgggtg tcgccagcgc cggtatgctg atttttggtt cgacgctgat tatgcagcac
     7621 acgcgccatc cttttgtgct ggcagctttg ttttctggcg ttggcattgc actgggcaac
     7681 gaatatgttc tggcaggcct gcattttgcc ttctcctcgc aaacgttatg gcaaggcgcc
     7741 ggggcgcttt ctggcatgat gctgatcgca cttacgcttt taatgccctc gaaaaaacac
     7801 gccatcgcac caatgccatt ggcaaaaacg gagcaacaga taatgagctg gtggttactg
     7861 gctattctgt acggccttgc gggatttggt tatatcatcg tcgcgactta cctgccgctc
     7921 atggcgaaag atgcaggctc accattgtta accgcccatc tctggacgtt agtcggctta
     7981 tcaattgtgc caggctgctt tggctggcta tgggcagcaa aacgttgggg ggctttgcct
     8041 tgcctgacgg cgaatttgct ggtgcaggct atcagtgtgc tgcttactct cgccagcgac
     8101 tctcctctct tgcttatcat tagtagtatc ggttttggcg gtacctttat gggcacgaca
     8161 tctctggtga tgactatcgc ccgccaactg agcgttccgg gaaatctcaa tcttttaggc
     8221 tttgtgacgc tcatttacgg cattggacaa atcctcggcc cggcgctgac cagtatgctc
     8281 ggcaatggaa catcggcgct tgccggcgcc actctctgcg gcgcggcggc gttgtttatt
     8341 gctgcgctta tcagcacagt acaattattt aagctgcaag tggtcacttc atgaggagtg
     8401 tttcccttac cactcaagaa attcgaccgc aaccgttgat gtcagccaga ctccgttttc
     8461 cgcttgccag aaaggaatgc ctcgtttcgc catttcctgt gctttgacgg ttaagacaac
     8521 cggcgatccg tgtcttgctc ccactttgcg tgccgttgct tcatcagcag agagatgcac
     8581 gtaatgacga tcccctgcaa tcagcccctg tttttttatc tcgtcaagga aacggcttgc
     8641 cgtaccgtgg tagagaaact gtggcggtgt tttttcggca aaggaaatag cgacctgcga
     8701 agtcgaatgc ccctgaaccg cgcggatgca cctaccatca ctggaataac taaaacgctt
     8761 tttatcgctg gttgcgacca cggtatccag caatgcacgg gttaatcgct tgcccgcttt
     8821 ttgggcacaa agtatgagct tatcgatatc cgcccatcct tcacggtcca gtacgatacc
     8881 aatcgcctcc ggtttgtgtc ggaggacaaa acttaaaaac ttgctcgtat ccgccagctc
     8941 tttttcgttg tgctttgcca tctcgttttc gtccctggat tttctgtttt tatttaccaa
     9001 tgtcattgtc actgggttta tgaattttct gcaactcaat cctgcgcaga accttttcca
     9061 cacagcgtaa tgtttcccct atgcaaaaaa cgcatagcca gaaacatact tgcattttcc
     9121 ccatacccac atggctgata aggtttttca gttccaggaa ttttcctggc tcaagcaaca
     9181 cacaaacaca aaaaaacaat ggggattgtt atgacacaac aaggggatgc tgtagccggg
     9241 gagctggcga cagaaaaagt gggcattaaa gggtatctgg ctttttttct caccataatc
     9301 tttttttccg gtgttttttc cggaacagac agctggtggc gcgtttttga tttttccgtt
     9361 ctgaacggat cgtttgggca actgcctggt gcgaacggag ccaccacctc atttcgcggc
     9421 gcgggtggtg cgggggctaa agatggcttt ctctttgcgc tggagctggc accgtcagtc
     9481 attctctctc tggggattat ttcaattacc gatggtcttg gtggattacg cgccgcgcag
     9541 cagttaatga cacctgtgct aaaaccgttg ttgggcattc cagggatctg ctcgctggcg
     9601 ctgatcgcta acttgcaaaa cactgatgcc gccgccggta tgaccaaaga gctggcgcag
     9661 gaaggtgaaa ttaccgaacg cgataaagtg attttcgccg catatcaaac cagcggtagc
     9721 gccatcatca ccaactactt ctcttccggc gtcgcggtgt tcgcctttct gggcacgtcc
     9781 gtaatcgtac ctttagccgt catcctggtg tttaaatttg tcggagccaa cattttgcgc
     9841 gtctggctta acttcgaaga acgtcgcaac ccaacgcaag gagcgcaagc atgacaactc
     9901 aggtccgcaa aaacgtcatg gatatgttta tcgatggcgc gcgccgtggc tttactatcg
     9961 ccacgaccaa cctgctgcca aacgtggtga tggcgtttgt catcattcag gcgctgaaaa
    10021 ttaccggcct gctcgactgg gtggggcata tctgtgaacc cgtaatggcg ctgtggggat
    10081 tacccggtga agcggcaacc gtccttttag ccgcattaat gagtatgggg ggcgcagtcg
    10141 gtgttgccgc cagtctggca accgctggcg cattaaccgg tcacgatgtc accgttttac
    10201 tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt cggtcgctgc ctggggacag
    10261 ccgaagtgaa tgccaaatat tacccgcata tcattacggt ctgcgtgatt aacgcattgc
    10321 tgtcgatctg ggttatgcag cttattgttt aacaaggagt taccatgatt gattataccg
    10381 cagccggttt taccctgctg cagggagcgc atttgtatgc gccggaagat cggggaattt
    10441 gcgatgtcct cgtcgctaac ggcaaaatta tcgccgttgc cagcaatatc ccttctgaca
    10501 ttgtaccgaa ctgcacggtt gtcgatctca gtgggcagat cctctgccca ggttttattg
    10561 atcaacacgt ccatttgatt ggcggtggcg gcgaagcagg tcccacgacg cgcacgccgg
    10621 aagtggcgct aagtcgcctg acggaagcgg gcgtcacgtc agtggttggt ctgctgggca
    10681 ccgactctat ctctcgccac ccggaatccc tgctcgccaa gacccgtgcg ctcaatgaag
    10741 aaggcatcag cgcctggatg ctgaccggcg cttatcatgt cccttcccgc actattacgg
    10801 gttccgtgga aaaagacgtg gcgattatcg atcgtgtgat tggcgtgaaa tgcgccatct
    10861 ctgatcaccg ttctgccgca ccggacgttt atcacctggc taatatggcg gcagaatccc
    10921 gcgttggcgg tttgctcggc agtaaacctg gcgtcaccgt gttccacatg ggcgacagta
    10981 aaaaggcgtt acagcctatt tatgacctgc tggaaaactg cgatgtgccg atcagcaagc
    11041 tgctgccgac ccacgttaac cgcaacgtac cgttgtttga gcaggcgctg gagttcgcgc
    11101 gcaaaggcgg caccatcgat atcaccagca gcattgacga accggtcgcc cctgccgaag
    11161 gtattgcccg cgccgttcag gcgggtattc cgctggcacg cgtcaccctc agctccgacg
    11221 gcaacggtag ccagccgttc ttcgatgacg aagggaattt aacccatatc ggtgttgcag
    11281 gttttgaaac gttgctggaa accgtgcagg tgctggtcaa agactatgat ttcagtatca
    11341 gcgatgccct gcgcccgctc accagtagcg tagccggttt ccttaacctg agcgggaaag
    11401 gcgaaattct gcctggcaat gacgcagact tactagtcat gacgccagaa ttgcgcattg
    11461 agcaggtata tgctcgcggc aaactgatgg tcaaagacgg caaagcctgc gtgaaaggaa
    11521 cgtttgaaac ggcttaaacg gttgtcagcc gccctaatgg acgtagaatg cccgcaaggg
    11581 cggctgacag agtaaaacgt aatggatgac tgttgtgcgg ttttgcataa tattgaaacc
    11641 aaatggcttt atgattttct gaccctggaa aaatgccgca atttttccca ggcggcagtc
    11701 agtcgtaacg tctcgcaacc ggcattcagc cgccgcatcc gcgcgctgga acaggcgatt
    11761 ggcgttgaat tgtttaaccg ccaggtgacg ccgctgcaac tctcagaaca agggaaaatc
    11821 ttccattcgc agatccgcca tctgttgcaa cagttagaaa gtaaccttgc agagctgcgt
    11881 ggcggcagcg attacgcgca acgtaaaatc aagatagccg ctgcacactc tctttccctc
    11941 ggtctgttac cgtccattat cagccagatg ccgccgctct ttacctgggc gattgaagct
    12001 attgatgtcg atgaagcagt cgataaactg cgtgaagggc aaagtgactg tattttttcc
    12061 tttcacgacg aagatttgct ggaagcaccg tttgaccaca ttcgcttatt tgaatctcaa
    12121 ttgttccccg tttgcgccag tgacgaatac ggagaagcaa tttttaacct cgcgcagcca
    12181 cactttccgt tactgaatta cagccgcaac tcctacatgg ggcgattgat taatcgcacc
    12241 ctgacgcgcc acagcgagtt aagtttcagc accttttttg tctcttcgat gagcgagcta
    12301 ttaaagcagg ttgccctcga cggctgtggg attgcctggc tgccggagta tgccatccaa
    12361 caagaaattc gcagcgggca actcgttgtg ctgaatcggg acgaactggt gatcccgatt
    12421 caggcctacg cataccgtat gaatacccgc atgaatccag ttgccgaacg cttctggcgt
    12481 gaactgcgcg agctggagat tgtgcttagc tgacattctc aagcgtcgca ctgcgccaac
    12541 cgccattatc ccagaccaga tcaaacgtta gcggttcatc gtcgtggtac agcattatct
    12601 caaggctgat cgccagagtg tggcagtacg cttctttcaa attaacctgc aacgccgcaa
    12661 tccgtggctc atgaaataac agtgcagaac gcagctgacg gcaataccag ctctggagat
    12721 gtttgtcgct cgggttatgc acttgccact cgggcatgcc tgaaacaagt aatgacggta
    12781 atgccgaatg aggagaatta aaaagagtga ggagatcgct gcataccgat ttacgcagca
    12841 atgacgtttt attgcctgaa atttcaggta aaacagcctg aagtgattgg cgcatcattt
    12901 tgcgcactcc tgacgtttag caacatatgc ggcttgccct gaacaccggg caccactgta
    12961 ttgatgctca ttttccatag ccattccctt ttgttcatta catccatgtc agtctgagac
    13021 aggcctgaat cctgtcagca aagtataaaa ccttacttgc ctatgaatat ctactgactt
    13081 ttcaggaaaa aagtcatgat ttgataaaaa tcatcaaact caaaaaaaaa ccgctagtat
    13141 attctgaata ttccgaatag ttacgtagat gaaaatactt gtcaccgccg ctacttaatt
    13201 ccggtggaaa attatttatt tccgcataat cagccatgta ttatttgaaa caattagttt
    13261 gtaaataata taaattgata acatatcaaa tacatcaaaa ataccattca taggcattat
    13321 taatatagac atcaatatag caacatcaaa ttccaattag taataagata tgctttttaa
    13381 ttgcatgaaa ataatttcaa atgagcgttt tttgatactt tagagtgttt cctacccttt
    13441 aatatcaccg tgtagagttg atatcactac aacagaaaca tgcatgtcat ttttttactt
    13501 cttttttact acagaaaaat ttatctattt ttctaaacat cggtgaaata ctcataaaag
    13561 gtaactatta tgtctatacc attaagcaat gcccttcaga gtcaaattat tacagataat
    13621 cacttccttc atcatccaaa agtcgacaat aagttaacac gtgaatatga atatacacgc
    13681 atggatacgg aaaatatcta tcttctgcct cttactcgtg gaaataacca taattacgat
    13741 ggaaaaagcg ttgtcgaaat tagaaaactt aatattggca aacagtcctg gccatttaat
    13801 tatgcgacag atacatgcag agaattcaat ggtattacaa caacaggacg tatgctctat
    13861 cgaaacttga aaatcacctc agctttagac gaaatatatg gtggcatatg taaaaaggcg
    13921 catgctgcta cagagttagc agaaggttta cggctaaatt tatttatgaa gagccctttc
    13981 gatcctgttg aggactatac agttcatgaa ataacattag gccctggtta caatgttcct
    14041 ggttatgccg gaaccacgat aggatatatt tcaacccttc cagcttctca ggcaaaaaga
    14101 tggaccaatg agcagccaag aattgatatc tatattgatc agataataac tgtctcaggg
    14161 gtggcaaatt cttccggatt cgcccttgcc gcattactta atgctaatat tggaatggga
    14221 aatgacccaa taattggtat agaagcctat cctggtaccg cggagatcca tgcaaaaatg
    14281 ggatataacg ttatccccgg tgatgaagat gcgccactca aacgcatgac tctgcaaccg
    14341 tcatctcttc cagaactgtt tgaattaaaa aatggtgaat ggaattatat tggcaaataa
    14401 ccactgagaa gaatgagtac taacattcag gttatataga tatttaaaca ataccctgca
    14461 atttacaaag taatactcat tgcgacttct taatttaaga aagcgcaagg aacgtttacc
    14521 cttgcgctta tcataatcag tcaggcgtcg actttatcca gcggccatga atcaaacaga
    14581 tagccgtcaa aataaatatc gtcaacgttc gagaattcca gcagacgttg cttaacgttt
    14641 tccagatgct gccacattgc cagcttcgcc gctcgggcat cttttttgat taacgcggca
    14701 aggatctgtt tgtgatcgcc caaccactct ttgcgataca ggctgtcatc cagatggctg
    14761 tgcaactgga tccacattgg attgttttcc cgccactgcc aggactgacg gaataactcc
    14821 accagcatgc tgttatgcgt tgcttcagca atggcgagat ggaactgcat gtcaccgctt
    14881 tcgctgctac ccggcgcgct ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc
    14941 attttgacga tatcttcgcg ggtagcctgc agggcggcaa attccgcgat gttgctctca
    15001 agaagctgtc gcgcctgcaa caattcaaag gggccggcat cattgcagac gttggcatcc
    15061 ggactgtctg tgttgtggct gcctgagcta tcaagaacat agataccggc accccggcgc
    15121 acttccacca gccctttgat ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg
    15181 acatcaagca tttctgcaat ttcacgctcc ggcggcagcc gttcgccagg attgtacggc
    15241 gtctttatga tcagatcgcg gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag
    15301 gtggcagatt tcatggtgtt gtgtccgtta ggtgcggtta atcaaatttt tgccggatgc
    15361 ggcgtgaacg ccttatccgg cctacaacgg gcaattttac ggcccccgtt aatctcaaac
    15421 tataccatta gcactggaag ttggcacgta caggtgggca aaaaaacgaa aactggtcaa
    15481 ccaaatatca gtttgattga ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc
    15541 cacacactcg cgcgcaccgc gttcgcacag ctgctgatat gccgccgtca ccgcgccaac
    15601 aaagtcggca ttctgcggca gatcatcggc aaaaataccg ctcaggccca gcagcgcttt
    15661 cacgcggtct gcgccctgat actgcgcgtt aatcttctgg aactcggcca gcatcgggtc
    15721 gaccacgtca atggcattac cctgctcatc cacgccctgg gtgtaacgca tccagccagc
    15781 cacgcccagc gccaggtgac gccagctgcc gccgttttgc aggtgcagac gcaccgggtc
    15841 cagcagacgc tgcggtaact tctggctgcc gtccatcgca atctgccagg tacggtgacg
    15901 cagagacggg ttgctgaaac gctcgatcag cagcgtcgca taggccttca ggtccgtacc
    15961 ctccggcata gacagcgttg gcgcttgttc ctgcatcatc agggcaaagg ccgctttgcg
    16021 ataatccggg ttagtcatgg tgtcggcaat agtttcatag ccgccgaggt aaccgaggta
    16081 tgccaggaaa gagtggctgc cgttcagcat acgcagcttc atcatttcga acggcacaac
    16141 gtctgcaacg aactgtgcgc ccactttatc ccagtccggg cgaccattaa cgaagttatc
    16201 ttcaatcacc cactgacgga acggttcgca ggcaatggcg cacgggtcgt aaacacccag
    16261 ctggtcagca atttcctgta aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat
    16321 ggtgcacggg aaggtgacgt tctcttcaat ccatgccgcc agctgcgggt cacgtgcctg
    16381 agccagcccc agtaccgcga ccttcgccac atgaccgttt tcacgcacgt tatcgcagga
    16441 catcaccgta aatgctttca gccctttttc acgacgcaga cgcaaggctt cgacgatgta
    16501 accaatcgcg gacttcggcg cagtcgggtt ttctagatcg tgcttgatca gcgggttatt
    16561 gagatccagt tgaccgcttg ccgcatcagc gcagtagcct ttttccgtga ccgttagaga
    16621 gacaatcgcc gtttgcggac gcgccatcgc gttgagaata ccttcgcagc cgtcgatttc
    16681 cggatgcagc gcttctttca tcgaaccgat aattttcagc tcggtgcttt ctgcgccttt
    16741 ttccgctacg gtgtacagca gttgctgttt tttcaggttt tcgatcagca cgcggtcgtt
    16801 gcctggcatc aggttaactt cgcagatgcc ccagtcgctg tcggtgcttt ccagcagatg
    16861 atgggtatac agcgcctgat gtgcgcggtg aaacgccccg caaccgagat gcacaatgcg
    16921 tgattccaga cgagaatgat cccatgacgg acgggcaacc ggcagattgc tgtcaacaat
    16981 agtagtcatc gtaaactcca gaatccgtct tcagagagtg acggcaagga agaaggaacc
    17041 ggcacggcgc gcagccatgc cggtggatat taacggctaa agaaagcgcg ctggatcgcc
    17101 agttcgacac cgcgaacttc ggccaggcct ttcagacgac caattgcgga gtaacctggg
    17161 ttggttttct tcttcaggtc gtccagcatc tgatgaccgt ggtccggacg catcgggatc
    17221 aggtcttctt tgccttccgc tttacgacgg tgttcttctt caacaatcgc tttcaccact
    17281 tcgtacatat caacgtcacc gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct
    17341 tcacgcatgg tggagcgcag atgggtgaag taaatacgcg gaccgaactg cttgatcata
    17401 tcaaccagat cgttgtcagc acgcacgccg taggaaccgg tgcacatggt gaaaccgttc
    17461 gccatgctgt ttacggtatc aaccatccac tgcatatctt caatggtgga aacaatgcgc
    17521 ggcaggccga ggatcgggcg cggcggatcg tccgggtgaa ccgccatacg cacgccaact
    17581 tcttcagcaa ctggaataat cgctttcagg aagacggcaa agttttcgcg cagcttcgct
    17641 ttatcgatat ctttgtacag ctccaggtgc ttacggaact ggtcgagggt atacccttct
    17701 tccgcgcccg gcagaccggc aatgatgtta cgcgtcagac gcgctttgtc ttcatcgctc
    17761 atagtggcga agcgttcagc tgcctgagca atttcttctt cggtgtaatc cgcttccgcg
    17821 cctggacgtt tcaggatatg catttcgaat gcagcgaatt cgatctggtc gaagcgcaga
    17881 gctttggagc cgtctggcag cacgtattcg aggtcagtac gggtccagtc gagcaccggc
    17941 atgaagttgt agcacacggt gcgaatgccg cactgcgcca ggttgcgcag agtctgctga
    18001 tagttagcga tccactgctc atagttgcca gtgtgggttt tgatatcttc gtgaattggt
    18061 acgctttcta cgacagacca caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg
    18121 atctcttcta cggaccatac ttcgccgttc gggatatggt gcagcgcggt aaccacgcca
    18181 gttgcgcccg cctgacggac atcagctaaa gaaaccagat cgtttgggcc gtaccagcgc
    18241 caggtctgtt ccatgatttt tcctctttca catagtgggt tccacagcaa agagacgcgg
    18301 aaccctggag ttgtttaggt atccggatga acaaccagac cgcagccgtg gcgttgcatc
    18361 ggatgagatg gcgtataagt tctacctttt ggtaagatat gtcaacccaa atcctgaaat
    18421 tggttaacca aatcacaaga atttcactct catggttgtt gctgcaaatt gcagcaaaag
    18481 gtaatcgatg gttattgatc agaggatgtg acattcatcg caacaatggt tgaccaattt
    18541 acataacatg tcgcccaaaa taacaacggt tcaaccaggt aaggtaaaac gttatgcatg
    18601 tgcttaacat tctctgggta gtattcggca ttggtctgat gctggtactg acaacgaccg
    18661 caggttcaga cacattcact gcattagcaa tgcctcttag attattttga cctgtcagta
    18721 aaagacacgt tgaaaacctg gggtctgctg gaactggtca actccgtggt tgggctgatt
    18781 attagcaagg tagcgtaaaa gaacaccaga gcctgccaat ggcaggctct gactgatgac
    18841 aaagtctaaa aatgtgcccg gacagtcccc tcgcccctcc ggggagaggg ttagggtgag
    18901 gggaacagac cagcaaagtg cgctttgttt atgccagatg cgacgctgac gcgtcttatc
    18961 tggcctacaa agggctaacg tgcaggtttt tagcttcagg taatattgcg taactgcatt
    19021 agcaatgccc tgtgatttct ttattgataa acaaaagtca cgccaataat cgattgcaca
    19081 ttccctgcag tcacctgccc tccggtacgt gcgtaatttg ccgttaatcc cagacttacc
    19141 gccgaagttc cta
//
LOCUS       DAJTOR010000071        19076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTOR010000071 DAJTOR010000000
VERSION     DAJTOR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.071"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(21..2468)
                     /gene="glgP"
                     /locus_tag="OO249_002172"
     CDS             complement(21..2468)
                     /gene="glgP"
                     /locus_tag="OO249_002172"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5636463.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDERQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2487..3920)
                     /gene="glgA"
                     /locus_tag="OO249_002173"
     CDS             complement(2487..3920)
                     /gene="glgA"
                     /locus_tag="OO249_002173"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5636464.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRASLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3920..5215)
                     /gene="glgC"
                     /locus_tag="OO249_002174"
     CDS             complement(3920..5215)
                     /gene="glgC"
                     /locus_tag="OO249_002174"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5636465.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5233..7206)
                     /gene="glgX"
                     /locus_tag="OO249_002175"
     CDS             complement(5233..7206)
                     /gene="glgX"
                     /locus_tag="OO249_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5636466.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7203..9389)
                     /gene="glgB"
                     /locus_tag="OO249_002176"
     CDS             complement(7203..9389)
                     /gene="glgB"
                     /locus_tag="OO249_002176"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5636467.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9662..10765)
                     /gene="asd"
                     /locus_tag="OO249_002177"
     CDS             complement(9662..10765)
                     /gene="asd"
                     /locus_tag="OO249_002177"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5636468.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10957..11550
                     /gene="yhgN"
                     /locus_tag="OO249_002178"
     CDS             10957..11550
                     /gene="yhgN"
                     /locus_tag="OO249_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5636469.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11607..12947)
                     /gene="gntU"
                     /locus_tag="OO249_002179"
     CDS             complement(11607..12947)
                     /gene="gntU"
                     /locus_tag="OO249_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5636470.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGPLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12951..13478)
                     /gene="gntK"
                     /locus_tag="OO249_002180"
     CDS             complement(12951..13478)
                     /gene="gntK"
                     /locus_tag="OO249_002180"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5636471.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMLRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13617..14612)
                     /gene="gntR"
                     /locus_tag="OO249_002181"
     CDS             complement(13617..14612)
                     /gene="gntR"
                     /locus_tag="OO249_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5636472.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14836..15531)
                     /gene="yhhW"
                     /locus_tag="OO249_002182"
     CDS             complement(14836..15531)
                     /gene="yhhW"
                     /locus_tag="OO249_002182"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5636473.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15654..16691)
                     /locus_tag="OO249_002183"
     CDS             complement(15654..16691)
                     /locus_tag="OO249_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5636474.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFISDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLSVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17023..17511
                     /gene="yhhY"
                     /locus_tag="OO249_002184"
     CDS             17023..17511
                     /gene="yhhY"
                     /locus_tag="OO249_002184"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5636475.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRSSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17748..18947
                     /locus_tag="OO249_002185"
     CDS             17748..18947
                     /locus_tag="OO249_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HCR5636476.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLSKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPMKNVMNASH
                     TIKHGAV"
BASE COUNT         4885 a         5046 c         4644 g         4501 t
ORIGIN      
        1 cgttctattt attggtgaac ttacaatctc accggatcga tatgccagat atgatcggcg
       61 tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt cagcatcgct
      121 tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg acaatcgaca
      181 tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag cgaatcaacc
      241 agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat ttgcgtcagc
      301 acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc
      361 agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc accgacatga
      421 tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc gttaagcgca
      481 aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg ttcagacaga
      541 tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat gaacacgact
      601 ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc attgatcaaa
      661 tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa aatattcacg
      721 cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt aatcacatgc
      781 aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac atcgaacaac
      841 gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag acgctttttg
      901 ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca gtgttgttgc
      961 agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc gtccagcacg
     1021 gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc gttggtgacg
     1081 ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg caccatcaga
     1141 ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc cagccaggcc
     1201 atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag cagatcggta
     1261 tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc aaagatgatc
     1321 tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc cagcgcctcg
     1381 ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa cgcgtcgtcc
     1441 cagctaaatt ggcgctcatc gatcagcaga cgcatcatct caggaatcga cagtaccgga
     1501 tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt tttatgcaac
     1561 tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccagaaagta ttcctgacgc
     1621 aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg agatacgttc
     1681 tcggagtggt ttttatcttc cactgccgcg aagtagtcac cttggttgaa tttaccgagg
     1741 ttaatttcgc tactggcttg cgcactccac aaacgcaacg tgttggtcgc gtcggtgtcg
     1801 taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc aatccagcgc
     1861 gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg cgtgttgtgg
     1921 cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc tttctggcta
     1981 ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta accgcgcccc
     2041 ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc caggccaccg
     2101 ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa ccccatcgct
     2161 tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt ggagagcgta
     2221 cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg cgacaactgg
     2281 gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa taacgttgcg
     2341 ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa catcagcttg
     2401 taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata tgtaaacgga
     2461 gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc acggtacgac
     2521 ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac aaaccgccac
     2581 agtgaagcac gagaccacag tacaaaagca cgtcgaatag cccgtaacag cgaccaggca
     2641 ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc aagagaacag
     2701 tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt accgtacttc
     2761 aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag aatgacgtcc
     2821 gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac gcccacctgg
     2881 ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg
     2941 agtagcgcca gctgcccgcc ctgctccaga agacccggta aggcttccag caccaggtcg
     3001 agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt atcgtcaacc
     3061 ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc caacgtatcg
     3121 cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt ctcgtccacg
     3181 ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag accttccata
     3241 ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac cgccgtaatg
     3301 tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa ttccagccca
     3361 tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc ataaaacatg
     3421 ccttgatagg ccaagttgtg cacagtaaac accgacttcg ccggacgccc gcgcgccgcc
     3481 agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc aggacgccag
     3541 aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc aaaacgcaat
     3601 acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg acgatcatag
     3661 agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa caacagcgtg
     3721 atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtcac gccacggcga
     3781 atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg
     3841 ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa catctctgaa
     3901 catacatgta aaacttgcat tatcgctcct gtttatgccc taacttccgt agcatttcgc
     3961 gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct tcctctgcgt
     4021 tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg cagcggcgca
     4081 gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca atgttgcaga
     4141 atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa ccggagatca
     4201 cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag cgatcctgca
     4261 cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg cgatcgtaca
     4321 tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa gctttcagcg
     4381 tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa gagagcggga
     4441 acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt
     4501 cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga tagtcggcgt
     4561 caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc attgacggtg
     4621 ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc gccataacgc
     4681 caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt
     4741 tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg tcgcccgcca
     4801 ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg gtgaccgcat
     4861 ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc agcagatcga
     4921 caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc tgcaccagag
     4981 tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg atgcagttag
     5041 acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt tttgctcgct
     5101 tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg gcaacagatt
     5161 tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta accatgacta
     5221 actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct gccagacagc
     5281 cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact ccccagcagg
     5341 taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc gatccgagag
     5401 cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag gttgagcata
     5461 tcgatttagc caacggacat tgccatcgcc ttcttcccac cagcgattct ccaccaaagc
     5521 gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac cactgcttgc
     5581 ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt tattgccatg
     5641 ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg agagcaacaa
     5701 cgttgttaac aaggcgtgaa tactgtcgcg ccgccgttca accaggtcaa gagaaccgcc
     5761 taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc gattttcttc
     5821 tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg taaaaccgtc
     5881 atgcgcagtg acgagattaa tcgcggcact cggcagacga ccattacgtt taaaaacatc
     5941 gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat gtagccagaa
     6001 acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg ggaaatttcc
     6061 cacctgataa ccaccagggg cgatatccca cggttcagca attaacttca cctgcgagag
     6121 caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact ctggcgtacg
     6181 gcccatgact gccgccagat caaagcggaa accatcgacg tgacaggttt ctacccaata
     6241 acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga gcgtgttgcc
     6301 gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc tacggttatc
     6361 gatcccacgc agtgagaata acgggccgtc gaggtccagt tccgcactat ggttgagcac
     6421 gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat cgcgaaactc
     6481 atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca tcgccaccgg
     6541 gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac tggcaaactg
     6601 cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt tgatcatcac
     6661 cggatgcccg agggctttat aagtgccacg gatctcgacc ggaatctccg ggtgcaagta
     6721 cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg tgcgcggcgg
     6781 ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg caatggcggc
     6841 gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat ctttaaattc
     6901 cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa agcgatgccc
     6961 ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg ggcgcgcatc
     7021 cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc gatgttcctg
     7081 gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga aaagtgtgaa
     7141 gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa tggcgagttg
     7201 tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagtgt caggcttagt
     7261 gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc attgcctgca
     7321 ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt gcctggctga
     7381 ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc aacgataatt
     7441 tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt gtcatccacc
     7501 accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt atggtggcgg
     7561 taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt atcgccacct
     7621 tccaacagat gccagtcgag gctggcgtca tggttccact cacggccctg ggcaaattcg
     7681 ttccccatga acagcagttt cttgcccggg aatgcccaca tccagccata gtaggcgcgc
     7741 aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga ttttttaccg
     7801 tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt gtagagaatc
     7861 ccgaaggtga gtttatcgtg atgatactga cgataaaccg ggtcgagctt catgtagtcc
     7921 agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc gcccatatcc
     7981 tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac cgcaccggaa
     8041 acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc aagattctcg
     8101 cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc gcgataaatc
     8161 attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat ccagtaaagc
     8221 gcgttaccga cgaggaagtt actgacttca cggcgaccat aattgtagat cagcgtattc
     8281 cagtcctgat gatagccttc gcgcggatcg ctgtgttcat acaagttcgt gccatcaaat
     8341 tcggcaagcg caaagtcatc ggtcgggaag tggcctggca cccagtcgag aatcacgttc
     8401 agacccgctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg
     8461 cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa gggatgctcg
     8521 ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg caccagttga
     8581 tcggccagct cgcggtagct caaccagaaa ttgttatcgg tgtggcgacg ccaggagccc
     8641 aggtgaactt cataaataga gattgacgca tcaaactgat tcgctttttt gcgctcttca
     8701 gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg gcgcatttgc
     8761 gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc aatcatctcg
     8821 tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccaaat gccgctctct
     8881 ttacgcagac gcatcgggtg acggcgaccg tcccagtagt tgaattgccc aaccaccgag
     8941 acccgacggg cgtttggagc ccaaacagag aaacgcgtac ctgtgacgcc atccatagta
     9001 tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga taatagccag
     9061 gcatccattt cctggattag cgggccaaaa cggtaaggat catcaatcag gttttgctga
     9121 ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg aatgacaccg
     9181 ctaaagaatc cccgtgagtc aagacactcc agttttgcga gtttgcgccc ggttttcggt
     9241 tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag tcccgcggtg
     9301 gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta
     9361 atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg tgtcacccca
     9421 tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg cgcagcactc
     9481 tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc atgtattcag
     9541 ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga aacatctgcg
     9601 cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta atgaataaag
     9661 attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac agcagctggt
     9721 cacccacggt aaaggctgac aggaactccg gtcccatatt cagcttacgc aggcggccta
     9781 ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata gtgatttccc
     9841 gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct tcgacggtcg
     9901 gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag cgcaatgccc
     9961 cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg atcttgttgg
    10021 tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt ttgtcgatcc
    10081 acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc tcaccgctac
    10141 gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg gtcgcgagtt
    10201 catctgccac atggccatac agatggccca tctgggttaa taactcacgc atatgtcgcg
    10261 caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca agatcattgg
    10321 cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg ccaacaaaag
    10381 tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg tcaagaatga
    10441 tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa ccttgccatc
    10501 cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga caggtcacaa
    10561 tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg ccagtggttc
    10621 cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca gggcgaatgg
    10681 cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc ataccgcgcc
    10741 agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg tgctgtataa
    10801 atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa agtcgcaagt
    10861 gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt tgttagcaca
    10921 cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat ttctgcagca
    10981 gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat gtccgtactg
    11041 aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct tattgctctc
    11101 ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct cagcctgcga
    11161 gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat taagatgatt
    11221 ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc atttatcgtg
    11281 ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat gttgttgtct
    11341 catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc ctggggcggc
    11401 acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga gaaaggggtg
    11461 aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca gatgttcctc
    11521 gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa gaacccggta
    11581 gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat catcccaacg
    11641 atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag cgtttcggct
    11701 tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg cgtcgttaac gtggctgaca
    11761 acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc ggagtagttc
    11821 agttgttcaa tgaccggcat caccagcccc accgccgtta aacaggcaac ggtggcagac
    11881 ccctgaatga tgcgcactgc agcggccagc acgaagcagg tgatagcaat cggcaggccc
    11941 atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag cacctgtttg
    12001 aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgtag cgcgtgaccg
    12061 caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc ataaatcgct
    12121 accagacaag caaccagaat cgcggtaaat ggatgaccaa taaactcgaa ccattcgtaa
    12181 gcagttgatc cttctggcac aaaacgagcg gcaatggttt tcagccccac cagcaccagc
    12241 ggcaacagga tcagcgacag gctgaatccg aaggacggca ttttgccttc gccgagatgc
    12301 ggttcgctga tgtcgtcagg aatatgcaac tccacgtaac ggctgatgaa attaccccac
    12361 agcggcccgg caataatcat tcccggaatt gccgcacaca gaccaatcag gatcatccag
    12421 ccaaagtcgg cattcatctg cgatgccagc aacatcggcg ctggtccagg caccagaaat
    12481 gccgccgctg ccgccacgcc tgcgaataat gggattacca gcttcaccag gttcgtaccg
    12541 gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa ctatcgccac ttcaaagaac
    12601 agcggtagcg cacagaccaa ccccgcaagg ccgatggcat aatgcgcgcg gctgtgaccg
    12661 aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcatg taagatcttg
    12721 ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggggcc tcccatccct
    12781 ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc agcccccatg
    12841 gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa aaacagcagc
    12901 agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac ttatttgcct
    12961 tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc gatatccacc
    13021 accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg cgtcaccaac
    13081 atttgggttt taaagaaatg gcctttgcgc gctttcaagc gactttcaat cacatcaaaa
    13141 tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa gtcgcgatag
    13201 tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgcag catagcaaac
    13261 gcggcgtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag cggttcgcca
    13321 gacgccattt tttcgatatt gcgccgtgga tggaggaaat caccatcaag aaacgcggca
    13381 tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga tacgcccatc
    13441 aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc acaatacaag
    13501 aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac aatatcagaa
    13561 gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt gtaggcttaa
    13621 atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt cacagattcg
    13681 ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc acgcggcgtc
    13741 agcacgctcg ctaaccgcgg ctccatcacc tggccaatgt catgaccgtg gaaaccggca
    13801 atcgccatat cgtcaggaac cttcaatccc agacgctgac attcaaacgc cgcgccgacc
    13861 gccaggtcat cgttggtaca gaacacgcca tccagctgcg gatattcccg ccgcgcctgg
    13921 cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac gctatacgac
    13981 accagacctg catccagcat cgcctgttcg tatcccttct gtttgatgat agtacgttcg
    14041 tcgagacgcg cgccgagata ggcaatgtgg caatgcccgc gagcaataat ggcggtggtc
    14101 atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca cggcgactgg
    14161 ctgtccatga gttccaccac cgggatcccc gccacttcaa tcatctttaa ggtgcgcggc
    14221 gtgtgggtac gttcggtgag gatcagtccg tcgatattcc atgagagcat ggattcgaga
    14281 cgttcttgct ccatttccgg tttataaccg tagtgagcca gcatggtctg ataaccgtgc
    14341 gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt ggtgagagaa
    14401 ggtaacagga cgccaatcgc ccggctggtg gcgttagaga ggatatcggg cgcacgattg
    14461 ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc gacggaaacc
    14521 tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac acggtcagcc
    14581 acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa gagatagatt
    14641 ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct tgtttacaat
    14701 tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc aaaaccccgg
    14761 aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg caggcttcaa
    14821 agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc tgtcgctatc
    14881 cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg tcgaggcttt
    14941 cacgccgtta atggtgacat tgcctttcac cacctggatc cagacgcggc gttcagcggc
    15001 aatctgatgc accgactgct catctttcag caacgcccag cggtacagtt ccatatcctg
    15061 atgcactttc aatgaaccat ctcgcgcatc cggcgagagc accagctgtt tgccctgtac
    15121 ggcatcgaag cgacgctgtt cataacgcgg cgtaataccg ttttcttcag gcatgatcca
    15181 gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt gacgaatacc
    15241 cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat tgcccatgct
    15301 gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat ctttatgcgg
    15361 gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc gcagcgcgga
    15421 gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc aggagtccag
    15481 ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca tttgtattcg
    15541 ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat gatagagggt
    15601 gaaaattgcc ccctctgttc aaaaaaattg aactattcga ggggccatca aacttaattc
    15661 gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat ttccagattg
    15721 gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt ttgatacaac
    15781 gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct gaccgtcacg
    15841 ccctcgtcat tgacatactc cggcacaccg accgaatcat ccgctgcgaa tcccggttcg
    15901 cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta tttaataaac
    15961 gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag atggctggtt
    16021 ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc gtcaggattg
    16081 gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc gaacagagaa
    16141 ataatctggt ccatcgtatg cacaccaagg ccataaaacg cgccatcctg cggcagccca
    16201 ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc aacaatctct
    16261 cccaacttgc cactttcaat cgcttttttc gctgtcagga agcaggagtc aaagcgacga
    16321 ttctgatacg gcgtgacgct cagccctttg cttttcgcca acgcaaacag ctccttcgcc
    16381 tgggcaagcg tcggggtgaa cggtttttcg accagcacat ttttcccggc ttccagcgcg
    16441 cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga caacaaccag cttaacatcg
    16501 ggatcgttta gtacttcgtc gagatcgctg atgaaatgga tatgggaata aatgggagcc
    16561 tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct atccttgcgg
    16621 ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat aaaggcgcag
    16681 ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg caaagcggac
    16741 gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa attgagaacg
    16801 aaagatcaaa aaaaagccag cacccggctg gctaagtaat actggaagca atgtgagcaa
    16861 tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata ataatcattc
    16921 tcattcgcgc ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca tttgaaagtc
    16981 aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga gatagtaata
    17041 cgccacgcag aaacacggga ttacgaggcc atcaggcaga ttcacgccca gccggaggtg
    17101 tattgcaaca cactacaggt gcctcatcct tccgatcata tgtggcagga gcgactcgcc
    17161 gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt gggccatctc
    17221 accattgacg tgcaacagcg cccacgcagc agtcatgttg ccgattttgg tatctgtgtc
    17281 gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga tgcgagagat gattgaaatg
    17341 tgcgacaact ggttgcgggt agatcgcatt gaactaaccg tgtttgtcga taacgccccg
    17401 gcaattaagg tctataaaaa atacggcttt gaaattgaag ggactggtaa gaagtacgca
    17461 ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc gggtgaagta agatagtgcc
    17521 ctttttctga gatggaaaaa gggtgtcatt caaaatcgac ataccttcct ttaaggtatt
    17581 tatttaccca atacatatat tgagatttaa ctcataatta attcctaaag tgtaatattt
    17641 tattttttaa tatatacgcc tacaatttcc tggagtaaat aaataacaat taacaagcat
    17701 aatattgcca ttgataaaat agcatgccat aaaaggactt ttcagggatg agtaatattg
    17761 tttacctgac agtaacggga gaacaacaag gaagcatctc cgcaggttgt gggacttctg
    17821 agtctacagg taatcgttgg cagagcgggc atgaggatga aatatttaca ttctcactct
    17881 taaataatat taataatacg gggcttggtt cacagttcca tggtataaca ttttgtaaat
    17941 taattgataa aagcactcca ttatttatta attccattaa caataatgaa caattattta
    18001 tgggatttga cttctatcga ataaatagat ttggtagatt ggaaaagtat tattatatac
    18061 aactaagagg cgctttttta tcggctattc atcaccagat cattgaaaac caactggata
    18121 cagaaacaat aactattagt tatgaattta tcctctgtca acatcttatc gcaaataccg
    18181 agttcagcta tttggcactc cctgaaaatt ataaccgatt gtttttacca aattcaaaaa
    18241 accaaacaaa taatcgtttc aaaacgttaa acagcaaagc tattggcagg ctacttgctg
    18301 ctggtggcgt atacaatggg aacattgaag gattcagaga tactgcggaa aaactgggtg
    18361 gagatgcaat aaaaggctat gatcaaatac taaatgaaaa aacagcgggc atagcgatag
    18421 caacagcatc tattctttta acaaagcgtt ctaatgttga tacatataca gaaataaata
    18481 gttacttagg caaacttaga ggtcaacaaa aacttcttga tggtatagac ataatagaaa
    18541 taatatacat taagagacct tcaaaagact tagctaactt acgaaaggag tttaataaaa
    18601 ctgtaagaaa aaattttctt atcaaacttt caaaaacctc cgaagcatct ggaagattca
    18661 acgccgaaga ccttttaaga atgagaaagg gcaatgttcc tctaaattat aatgttcacc
    18721 ataaactatc tctagatgat ggtggtacta atgatttcga aaatttagta ttaatcgaaa
    18781 acgaaccata tcataaagtt tttactaaca tgcaatcacg aatagctaag ggaatattag
    18841 taggtgaaag caaaatcact ccctgggcca ttccatctgg ctcaatttat cctcccatga
    18901 aaaatgtaat gaacgcatcc cacacaatta aacacggagc agtatgatgg ttaaaagggt
    18961 gaaactggca ggtgtggagc ctcgcagatt tccggcatca tagtgcccaa tacgggatgc
    19021 ataaagctcc ctcagactgg aaactccaca aatgaaatat acaccggttg gcgttg
//
LOCUS       DAJTOR010000072        18930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTOR010000072 DAJTOR010000000
VERSION     DAJTOR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.072"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..644
                     /gene="rihB"
                     /locus_tag="OO249_002186"
     CDS             <1..644
                     /gene="rihB"
                     /locus_tag="OO249_002186"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5636477.1"
                     /translation="STHAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKI
                     REIVLMGGAYGTGNFTPSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVI
                     ARMERAGGPAGELFSDIMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYV
                     EVDVNSGPCYGRTVCDELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            744..1994
                     /locus_tag="OO249_002187"
     CDS             744..1994
                     /locus_tag="OO249_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5636478.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPAGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDHLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2050..3138)
                     /locus_tag="OO249_002188"
     CDS             complement(2050..3138)
                     /locus_tag="OO249_002188"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5636479.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGIADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGAGDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3141..3998)
                     /gene="nfo"
                     /locus_tag="OO249_002189"
     CDS             complement(3141..3998)
                     /gene="nfo"
                     /locus_tag="OO249_002189"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5636480.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4072..5121)
                     /locus_tag="OO249_002190"
     CDS             complement(4072..5121)
                     /locus_tag="OO249_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5636481.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIIVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5221..6102
                     /locus_tag="OO249_002191"
     CDS             5221..6102
                     /locus_tag="OO249_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5636482.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANIPR"
     gene            6307..7776
                     /gene="lysP"
                     /locus_tag="OO249_002192"
     CDS             6307..7776
                     /gene="lysP"
                     /locus_tag="OO249_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5636483.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8069..10049
                     /gene="cirA"
                     /locus_tag="OO249_002193"
                     /pseudo
     CDS             8069..10049
                     /gene="cirA"
                     /locus_tag="OO249_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(10081..10917)
                     /gene="yeiG"
                     /locus_tag="OO249_002194"
     CDS             complement(10081..10917)
                     /gene="yeiG"
                     /locus_tag="OO249_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR5636484.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11175..11843
                     /gene="folE"
                     /locus_tag="OO249_002195"
     CDS             11175..11843
                     /gene="folE"
                     /locus_tag="OO249_002195"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5636485.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11860..13017
                     /locus_tag="OO249_002196"
     CDS             11860..13017
                     /locus_tag="OO249_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5636486.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13159..14199
                     /gene="galS"
                     /locus_tag="OO249_002197"
     CDS             13159..14199
                     /gene="galS"
                     /locus_tag="OO249_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5636487.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14479..15477
                     /gene="mglB"
                     /locus_tag="OO249_002198"
     CDS             14479..15477
                     /gene="mglB"
                     /locus_tag="OO249_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5636488.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15538..17058
                     /gene="mglA"
                     /locus_tag="OO249_002199"
     CDS             15538..17058
                     /gene="mglA"
                     /locus_tag="OO249_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5636489.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDKSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17074..18084
                     /gene="mglC"
                     /locus_tag="OO249_002200"
     CDS             17074..18084
                     /gene="mglC"
                     /locus_tag="OO249_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5636490.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPCWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18327..>18930)
                     /locus_tag="OO249_002201"
     CDS             complement(18327..>18930)
                     /locus_tag="OO249_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5636491.1"
                     /translation="NQKIGMPIVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFP
                     ISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGNRIVEDMASGLSHYLADQGFDSLQ
                     EMVGLANNNIVPAEDLDRSYIVYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTP
                     HCNTEKCVGCLLCGHVCPVGCIELGEVKFKKGEKEHPVTL"
BASE COUNT         4516 a         4657 c         5032 g         4725 t
ORIGIN      
        1 aaagtaccca cgcggtgaaa tacatcattg ataccctgat ggcaagcgat ggcgatatca
       61 ctctggtgcc ggttggtccg ctttcaaata tcgcggtggc aatgcgtatg caacccgcga
      121 tcctgcccaa aatccgtgaa attgtgctaa tgggcggcgc ttacggtaca ggcaacttca
      181 cgccatctgc cgagttcaac atctttgctg atccggaagc agcccgcgtg gtgttcacct
      241 ccggcgttcc attagtgatg atgggcctcg atctcaccaa ccagactgtt tgcaccccgg
      301 acgtgattgc acggatggaa agggcaggcg gccctgccgg agagctgttc agcgacatca
      361 tgaacttcac tctcaaaacg cagttcgaaa actacggcct ggctggcggc ccggtgcacg
      421 acgccacctg catcggttat ctgattaacc ctgatggcat taaaacccag gagatgtacg
      481 tcgaagtgga cgtcaacagt ggcccttgct atgggcgtac cgtctgcgac gagctgggcg
      541 ttcttggcaa gcccgctaat accaaagtcg gcatcactat tgataccgac tggttctggg
      601 gcttagtcga agagtgcgtg cgcggctaca tcaaaaccca ttaagtgttg ataacaagcc
      661 gggtcagtat cgcaagatcc aagcccggct atttctgatg ggcagaaagc cgtcgcccaa
      721 tatgacatga agagataata actatggatg tcatgagaag tgttctggga atggtggtat
      781 tgctgacgat tgcgtttttg ctgtcagtaa acaagaagaa gatcagcctg cgtaccgttg
      841 gcgcggcgtt agtgttacag gtcgtgattg gcgggattat gctttggtta ccggcagggc
      901 gttgggtcgc tgaaaaagtc gcttttggcg tgcataaagt gatggcgtac agcgacgcgg
      961 gtagcgcatt tatcttcggt tcgctggtcg ggccgaaaat ggacacgctg tttgatggcg
     1021 caggatttat ctttggtttc agggtattac cggcaattat cttcgtcact gcactggtga
     1081 gtattctcta ctacatcggt gtgatgggga ttttaattcg cattctcggc ggtatattcc
     1141 agaaagcatt aaatatcagc aagattgagt cattcgtcgc ggtcaccacc attttcctcg
     1201 ggcaaaacga aattccggcg atcgtcaaac cctttatcga tcatctgaat cgcaatgaat
     1261 tatttacagc gatttgtagt ggcatggcct cgattgctgg ttcgacaatg attggttacg
     1321 ccgccctggg cgtacctgtg gaatatttgc tggcggcatc gttaatggcg atcccaggcg
     1381 ggatcttgtt tgctcgcctg ttaagcccgg ctacggaatc ttcgcaggtt tcctttaata
     1441 acctctcttt caccgaaaca ccgccaaaaa gcattattga agccgctgcg acaggggcaa
     1501 tgaccgggct gaaaatcgcc gccggtgtag cgacagttgt tatggcattt gtcgccatca
     1561 ttgcgttaat taatggtatt atcggcggcg ttggcggctg gtttggtttt gcacatgcct
     1621 cgctggagtc cattttaggt tacctgttgg ccccattggc gtgggtgatg ggggttgact
     1681 ggagtgatgc aaatcttgcc gggagtttga ttgggcagaa gctggcgatc aatgaatttg
     1741 tcgcttatct caatttctcg ccatatctgc aaacgggtgg cactctggat gctaaaaccg
     1801 tggcgattat ttctttcgcg ttgtgcggtt tcgctaactt tggttctatc ggggtggtgg
     1861 tgggggcgtt ttctgcggtt gcgccacacc gtgcgccgga aatcgcccaa cttggtttac
     1921 gcgcgctggc ggcggcgaca ctttctaacc tgatgagtgc tactattgca ggattcttta
     1981 ttggtttagc gtagtgcgca atttatgagc cggataagat gcggtcaagc gtcgaatccg
     2041 gcaacattgt tacaccattg gcactaatga aagcgcgtta tcggcagaca gggtagggtt
     2101 gtttaagctg ccgctggcgc gcgaaattgc cgcgcaggct accgcaaaac gggcgctgtc
     2161 gcggaaactg tatccttcaa gaaagctata taccaggccc gccataaaac catcgccagc
     2221 accaaaactg tctactgtcg tatgcgctgg cgcggtcagc aaaaattgtt ctccatcctt
     2281 ttcgctgcaa taaactgact catcgggcaa ataaacaaac agttgctgaa caccttgctg
     2341 atgcaatgca ttcactgcgg cattacggtc agcatcgctg gtgatcgcct gtccccataa
     2401 aatttccagc tccggtaaag tgggtttcag ggtgtgaata tgcgccagcc agtgtttgat
     2461 tttgcccgct ttgaattctg aaacggtatc gacaaacacc gggatttcat cagcgagggt
     2521 aaagacccat tccagcgcct cggctgtcag gttgcaatca gccagtacca cgcccgcatg
     2581 acgaagtaaa tcgcgcgacc cgttcagtag ttgcggtgtc aactgttcca gcagatgggt
     2641 atcgttaatc gccagcacgg tttgatcgtc tcgattggca attgccagat acgtcgatgt
     2701 gctttgacca tgcaaacgaa cgcagccgga gacattcacg ccggcacggc gcgtttcttc
     2761 caggagcatt tcgccataaa agtcatcgcc aatcactgaa agcaaatgga cgtcacggcc
     2821 taacagcgcc agattgtggg cgatgttgcg tcccacgccg cccgccgagc aatgaattgt
     2881 accgggatga gaagccgctt gcgggtaacg gatatccgcg atcccgcgaa tatccatatt
     2941 gattgtcccc accactacgc agtattcctg ctcggtgaga atgtaacctt tgcctttaat
     3001 ccgccctttg cgcattaaat ccataatatg cgccgcaacg cgcgaacgac tgatttgcag
     3061 catgtccgca atttcgttct gctgaatcag cgggttacgc cgtaaaattg caaggatctc
     3121 cttttcccgg ttattcatct tcaggctacc gctttttcag tttgttgcgc tttcagccag
     3181 gcgatctctt ctgcccagat atccgggttg atggtttcaa ggatcagcgg aatgccgtcg
     3241 aaacggtcgt cctgcatgat ccagcggaac gcatcatgac cgatattgcc ttcaccgagg
     3301 ctatgatggc ggtcaacgcg gctgccaaag gtgcttttcg catcgttaag gtgcatcccg
     3361 cgcagatact taaagccaac aatacgggca aaatccgcga acgttttctc gcattcggct
     3421 ggagtacgca aatcataccc ggcagcgaaa gcatggcagg tatcaatgca gacgccgaca
     3481 cgggatttat cttccacgcc gtcgataatc gccgcgagat gttcgaattt aaaccctaag
     3541 ttactgccct gaccggcggt gttttcaatc accgctgtta caccttgagt tttatccagc
     3601 gcaatgttga tggattcggc aatacgcgca aggcaatcct cttctgaaat ctgcatcaga
     3661 tggctgccag ggtggaagtt gagcaaagaa agccccagtt gttcgcaacg ctgcatttca
     3721 tctataaagg catcgcgcga tttttccaga gcttcagtga ccggatgtcc gaggttaatc
     3781 agatagctgt cgtggggaag aatttgcgcc gatgtgtagt gatatttttc acaggctgct
     3841 ttgaattcat cgatggtttg cgtcgtgagc ggtgcggcac gccactgacg ttggtttttg
     3901 gtgaacaagg caaacgcggt tgcgtcgatt tcggcggcgc gaattgcggc atttgccaga
     3961 ccgccagcag cactaacgtg cgctccaatg tatttcatgc gaggactcct gttaaacccg
     4021 ctggaggaaa acggtaatga tagcgggtta acaggagcaa gatgtagtgg tttatgcgat
     4081 gacgctttga atcacatagt taatcgcacc accaccaaca atcagccagg caaacagtac
     4141 cagtgccatc agcagaggtt tcgccccggc ttttttcagc gcgctgacgt gagtggtcag
     4201 acccagcgcc gccatcgcca ttgccagcag gaaggtatcc agcgttacca gcatgttcac
     4261 cacgctctgc ggtaacaggt ggaacgagtt aaagatggca acgatgatga acaagatggc
     4321 aaaccacgga atagtgattt tgcttttctc gccgctgttc gccccagaca gctgtttaac
     4381 acgcgccgcc agcaggatga ggaacggagc cagcatcatc acgcgcagca ttttggaaat
     4441 aactgctgcg ttttccgcat ccgggctgat ggcatgacct gccgccacca cctgcgccac
     4501 ttcgtgcaca gtagaaccaa tgtagatacc gaaagtttcc ggactaaacc attgagacat
     4561 cagcggatat atcgccgggt agaggaaaat cgcgacggtc ccgaagataa caacggttgc
     4621 aacagccacg gttactttac tggcttccgc tttcactacc ggctcagtcg ccagtaccgc
     4681 ggcagcacca cagatactgc taccggcacc gatcaaccag ctggtgtgct tatccagacc
     4741 aaacactttc tgccccagga agcaagccag caggaaggta ctggacagcg tcaacacgtc
     4801 aatgatgatc ccactgatac cgacatcggc aatttgcgag aacgtcagac ggaagccata
     4861 aagaatgata cccagacgta ataaatattg cttggcaaac agcacaccac cgtcacagct
     4921 tttccagatg tgcggataga tggtgttgcc taaaaccatc cccaacaaga ttgcgagggt
     4981 gagggcacta aacccggcac ccgcaaccgc gggaatggaa ccaccccaca gggcgacccc
     5041 ggtgataact gcactcaggg ctaaccccgg aataaaatgc cacagtgtac gatgttgttt
     5101 ctgtaaggtg atattcgtca taaccctctc cttataaccg ggcataaggt tacgactaac
     5161 tggtttaaaa ataaaattga ttatatattt ataattaatc tttataagtg gtaagcgact
     5221 atgcacatca ccctccggca gttggaagtt tttgcagaag tattgaaaag tggatcaacc
     5281 acccaggcgt cggtgatgct ggcgttgtcg caatcagcag tgagcgcagc cttgaccgac
     5341 ctggaagggc agcttggcgt gcaactgttt gatcgcgtgg ggaaaagact ggttgttaat
     5401 gaacacgggc ggctgctcta tccgcgtgcg ttggcattgc ttgaacaggc ggttgaaatc
     5461 gaacaactgt ttcgcgaaga caacggcgcg attcgtatct atgccagtag taccatcggt
     5521 aactacattc tgcctgcagt tatcgcccgt tatcgccatg attatccgca gttgccgatt
     5581 gaacttagcg ttgggaacag ccaggacgtg atgcaagcgg tgctggattt ccgcgttgat
     5641 attggcttta ttgaaggacc gtgccacagc actgaaatca tttctgaacc gtggctggaa
     5701 gacgagctgg tggttttcgc cgcgccgact tcgccgttgg ctcgtggtcc ggtcacctta
     5761 gaacagctgg ccgctgcgcc gtggatcctg cgtgaacgcg gttccggcac gcgggagatt
     5821 gtcgattatc tattgctgtc acatttaccg aagtttgaga tggcgatgga attaggtaac
     5881 tccgaggcaa tcaaacatgc ggtgcgtcat gggttgggaa ttagttgcct gtcgcgacgg
     5941 gtgattgaag atcaattgca ggcaggcaca ttaagtgaag ttgcggtccc tctgccgcgc
     6001 ctgatgcgta cgttgtggcg tatacatcat cggcaaaaac acctttccaa cgcgctacgg
     6061 cgctttctgg actattgcga tcccgcgaat ataccgcgtt aagttgctgt acaagaacat
     6121 gctggtgctg tgtcgatttc gtgacgcagc gccctcagca tgcattcgcc agaaaagaga
     6181 ttggctgctt tacttataat cccagggcga tcatgaaggt gtcttataac cgtgtatttc
     6241 tgccggaagg attgccaatc gtctgctaca atcgcgcctc atttttaaga tggatagcat
     6301 ttttgtatgg tttccgaaac taaaaccaca gaagcgccgg gcttacgccg tgaattaaag
     6361 gcgcgtcacc tgacgatgat tgccattggc ggttccatcg gtacaggtct ttttgttgcc
     6421 tctggcgcaa cgatttctca ggcaggtccg ggcggggcat tgctctcgta tatgctgatt
     6481 ggcctgatgg tttacttcct gatgaccagt ctcggtgaac tggctgcata tatgccggtt
     6541 tccggttcgt ttgccactta cggtcagaac tatgttgaag aaggctttgg cttcgcgctg
     6601 ggctggaact actggtacaa ctgggcggtg actatcgccg ttgacctggt tgcagctcag
     6661 ctggtcatga gctggtggtt cccggataca ccgggctgga tctggagtgc gttgttcctc
     6721 ggcgttatct tcctgctgaa ctacatctca gttcgtggct ttggtgaagc ggaatactgg
     6781 ttctcactga tcaaagtcac gacagttatt gtctttatca tcgttggcgt gctgatgatt
     6841 atcggtatct tcaaaggcgc gcagcctgcg ggctggagca actggacaat cggcgaagcg
     6901 ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta tgattgtcgg cttctctttc
     6961 cagggaaccg agctgatcgg tattgctgca ggcgagtccg aagatccggc gaaaaacatt
     7021 ccacgcgcgg tacgtcaggt gttctggcga atcctgttgt tctatgtgtt cgcgatcctg
     7081 attatcagcc tgattattcc gtacaccgat ccgagcctgc tgcgtaacga tgttaaagac
     7141 atcagcgtta gtccgttcac cctggtgttc cagcacgcgg gtctgctctc tgcggcggcg
     7201 gtgatgaacg cagttattct gacggcggtg ctgtcagccg gtaactccgg tatgtatgcg
     7261 tctactcgta tgctgtacac cctggcgtgt gacggtaaag cgccgcgcat tttcgctaaa
     7321 ctgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga cgacggtgat tgccggtctg
     7381 tgcttcctga cctccatgtt tggcaaccag acggtatacc tgtggctgct gaacacctcc
     7441 gggatgacgg gttttatcgc ctggctgggg attgccatta gccactatcg cttccgtcgc
     7501 ggttacgtat tgcagggaca cgacattaac gatctgccgt accgttcagg tttcttccca
     7561 ctggggccga tcttcgcatt cattctgtgt ctgattatca ctttgggcca gaactacgaa
     7621 gcgttcctga aagataccat tgactggggc ggcgtagcgg caacgtatat tggtatcccg
     7681 ctgttcctga ttatttggtt cggctacaag ctgattaaag gaactcactt cgtacgctac
     7741 agcgaaatga agttcccgca gaacgataag aaataagttt cctcccttcc ttgctaagcc
     7801 ctctcaaccg agagggcttt ttcaattcca tttccctgac aaatcatgcg gatataaaat
     7861 ttaacatttg gattgataat tgttatcgtt tgcattatcg ttacgccgca atcaaaaaag
     7921 gctgacaaat cagaggctgt tccggctttc tgggatgatc acctgcatac aaataagtcc
     7981 accgcgatgc tgccgtacgc aaggggacgt gaagaaggtg tgagcgataa cccattttat
     8041 tttcgtagtt acctcattga gatatggaat gtttaggttg aaccctttcg tacgggtcag
     8101 gctgtgtttg tccgctattt cttgtgcatg gcctgtgtta gcggtcgatg atgatggcga
     8161 aacgatggtt gtcactgcat cttccgttga acaaaacctc aaagatgcac ccgccagtat
     8221 cagcgtcatt acccaggaag acctgcagcg aaaaccggta cagaatctga aggatgtcct
     8281 caaagaagtg cctggcgtac aactgacgaa cgaaggggat aaccgtaagg gcgttagtat
     8341 tcgtggtctg gacagcagct acaccctgat tctcgtcgac ggtaaacgcg ttaactcccg
     8401 caatgccgtc ttccgccaca atgatttcga tctgaactgg atcccggtcg attccatcga
     8461 acgtattgaa gtggttcgtg gcccgatgtc gtcgctatac ggctccgatg cgctcggcgg
     8521 tgtagtgaat atcatcacca aaaaaatcgg tcagaaatgg tcgggtaccg ttaccgtcga
     8581 taccaccatt caggaacatc gcgatcgcgg tgatacctat aacggtcagt tctttaccag
     8641 tggaccatta attgatggtg tgctgggaat gaaagcttac ggcagcctgg caaaacgtga
     8701 aaaggatgac ccgcaaaact caacgaccac cgataccgga gaaatgccgc gtattgaagg
     8761 attctccagc cgcgacggca atgtcgaatt tgcctggaca ccgaatcaaa atcacgattt
     8821 tactgccgga tacggtttcg accgtcagga actcgctgga caaaaaccgc ctggaacgcc
     8881 agaactactc cgtcagccat aatgggcgtt gggattacgg caccagcgaa ctgaaatact
     8941 acggtgagaa agtcgagaac aaaaaccctg gcaacagcag cccgataact tccgaaagca
     9001 atacggtcga cggcaaatac acgttgccgc tgacggcgat taatcagttt ctcacggttg
     9061 gcggtgaatg gcgtcacgac aaacttagcg atgcggtgaa cctgaccggg ggaaccagct
     9121 ccaaaacgtc tgccagccag tacgcgctgt ttgtggaaga tgaatggcgg atcttcgagc
     9181 cgctggcgct gacgaccggc gtgcgtatgg acgatcacga aacctacggt gaacactgga
     9241 gtccgcgtgc ctacctggtt tataacgcca ccgacaccgt aacggtgaaa gggggctggg
     9301 cgacggcatt taaagcacct tctctgttgc aacttagccc tgactggacg agcaattcct
     9361 gccgtggcgc atgtaagatt gtgggtagcc cggatctgaa accagaaacc agcgaaagtt
     9421 gggagctggg gctttactac atgggtgaag aaggctggct ggaagggggg gaatccagcg
     9481 ttaccgtttt ccgtaacgat gtgaaagatc gtatcagcat cagccgtacg tctgacgtca
     9541 acgctgcacc gggctaccaa aactttgttg gttttgagac gggcgctaac ggacggcgta
     9601 taccggtatt tagctactac aacgttaaca aagctcgtat tcagggcgtg gaaaccgaac
     9661 tgaaaattcc gttcaacgat gaatggaaac tgtcgatcaa ctacacctac aacgatggtc
     9721 gtgatgtcag caacggcgaa aacaaaccgc tatccgatct gccgttccat actgctaacg
     9781 gtacgctgga ctggaaaccg ctggcgctgg aagactggtc attctatgtt tctggtcact
     9841 ataccgggca gaaacgcgcc gacagcgcga cggctaaaac accgggcggt tataccatct
     9901 ggaataccgg cgcggcctgg caggtgacta aagacgtcaa actgcgcgca ggcgtgctga
     9961 accttggcga caaggatctc agtcgtgacg actacagcta taacgaagac ggacgtcgtt
    10021 actttatggc agtggattat cgcttctgat gagaagatgc ccggcgaacc gggcggactt
    10081 tcacttcagt aaatactgcg catggaagcg caggtgatcc tctataaaag aggcgatgaa
    10141 gtagtaactg tgatcatatc ccggctgaat acgcagcgtc atcggccacg ctttctggcg
    10201 cgcggcttct gccagtaccg caggttgcaa ctggtcggca agaaactgat cattatcgcc
    10261 ctgatcgata agcgtcggga tcgcatcctg cgcgttactg gcatacatca gcgcgcaact
    10321 gtcccattcc agccatgcat ttttgtcctc acctaaatag ctgctaaacg ctttgattcc
    10381 ccacgggacg ctgcacggat tcacaattgg cgcaaaggcc gaaacgctgg tgtatttacc
    10441 cggatttttc agcgccataa tcagcgcacc gtgaccaccc attgagtgac cgctaatggc
    10501 gcagcggtcg ctgacattaa attgcgactg aaccagcgcc ggtaattcat cgcgcagata
    10561 atcatacatc cggtaatgcg tcgcccacgg cggttgcgtg gcattaagat aaaagcctgc
    10621 gccctggcct aaatcgtagc catcgtcgtt ggcaaccttt tcgccgcgcg ggctggtgtc
    10681 tggcatcacc agtacaatcc ccagttctgc cgccacgcgc tgggcacccg ccttggtggt
    10741 gaagttctcg tcattgcagg ttaatccgga aagccagtac agcactggtg gcggggtgtg
    10801 atcacgaggg ggtgggagaa agatactgaa cgtcatcggg cagtttaagg tactggagtc
    10861 gtgtcgccag cgttgctgcc agccttcaaa acagcggtgc tcttcgagca tttccatgcg
    10921 tggctccttg ttgtgttgtt tgcaatttct tcatcatacc gtttattcaa tgtgctgtga
    10981 gtaactttca cttccgtatt tgcataacga tgttttaaca tctgctgatg aaaggcagcg
    11041 gcaattacaa taattatcgc tgtgaatact ggattatgtg cgccgcctca cgcacaataa
    11101 tcaggctgta aatcagctta ataactttgc ccccacgcag ggcggaggcg tcacacctgc
    11161 aggagaaatc ataaatgcca tcactcagta aagaagcggc cctggttcat gaagcgttag
    11221 ttgcgcgagg actggaaaca ccgctgcgcc cgcccgtgca tgaaatggat aacgaaacgc
    11281 gcaaaagcct tattgctggt catatgaccg aaatcatgca gctgctgaat ctcgacctgg
    11341 ctgatgacag tttgatggaa acgccgcatc gcatcgctaa aatgtatgtc gatgaaattt
    11401 tctccggtct ggattacgcc aacttcccga aaatcaccct cattgaaaac aaaatgaagg
    11461 tcgatgaaat ggtcaccgtg cgcgatatca ctctgaccag tacctgtgaa caccattttg
    11521 ttaccatcga tggcaaagcg acggtggctt atatcccgaa agattcggtg atcggtctgt
    11581 caaaaattaa ccgtatcgtg cagttctttg cccagcgtcc gcaggtgcag gaacgtctga
    11641 cgcagcaaat tcttattgcg ctacaaacgc tgctgggcac caataacgtg gctgtctcca
    11701 ttgatgcggt gcattactgc gtgaaagcgc gtggcatccg cgatgcaacc agtgccacaa
    11761 caacgacctc tcttggtgga ttgttcaaat ccagtcagaa tacgcgccac gagtttctgc
    11821 gcgctgtgcg tcatcacaac tgattaaaag gcaggaacca tggagcgcaa cgtaacgctc
    11881 gattttgttc gcggcgtcgc cattctgggg atcctgctat taaacatcag cgcctttggg
    11941 ctaccaaagg cggcttatct caatcccgcc tggtacggcg ctattacgcc gcaggatgca
    12001 tggacctggg catttctcga tctcatcggc caggtgaaat tcctcacgct ttttgcgctg
    12061 ctgtttggtg cgggcctgca aatgttgctg ccccgtggca gacgctggat ccagtcgcgg
    12121 ttaacgctgt tagtcttgct gggttttatt cacggtttat tgttctggga cggcgatatt
    12181 ctgctggctt acgggctggt gggcttaatc tgctggcggc tggtgcgcga tgcgccatcg
    12241 gtaaaaagcc tgtttaatac aggcgtcatg ctttatctgg tggggcttgg cgttttgctg
    12301 ttattggggc tgatttccga cagccagacc agccgcgcct ggacgccgga tgcatcggct
    12361 attttgtatg aaaaatactg gaagcttcac ggcggcgttg aagcgatcag taatcgtgcc
    12421 gatggtgttg gcaacagttt actggcactg ggcgcacagt atggctggca actggcaggg
    12481 atgatgctca ttggtgcggc gctgatgcgc agcggctggc tgaaagggca gttcagctta
    12541 cgtcactatc gtcgtactgg ttttgtgctg gtggcgattg gggtgaccat taaccttcct
    12601 gccatcgccc tgcaatggca gctggactgg gcgtatcgct ggtgtgcctt cttacttcaa
    12661 atgccgcggg aactgagtgc gccgtttcag gcgattggct atgcgtcgct gttttatggc
    12721 ttctggccgc aattgagccg ctttaagctg gtgcttgcga tcgcctgcgt cggacggatg
    12781 gcgctgacca actatctatt gcaaacgctg atttgtacca tgctttttta ccacctcggt
    12841 ttgtttatgc attttgaccg cctggagctg ctggcgtttg ttattccggt atggctggcg
    12901 aatatcctct tctctgttat ctggctgcgt tacttccgcc aggggccggt ggaatggctc
    12961 tggcgtcagt taactttgcg tgctgccgga acggcaatat ctaaaacatc aagataacga
    13021 tctggatcac attcgttaac aaaacggctg taaccgtttc cattgctgtg actcgattca
    13081 cgaagtcctg tattcagtgc tgacaaaata gccgccagca agcagtcatt tactgcaatc
    13141 tcataacagg tagtgaatat gatcaccatt cgtgatgtag cgcgtcaggc tggcgtctct
    13201 gtggcaacgg tttcccgggt gctcaataac agcacgctgg tcagtgccga cacgcgtgaa
    13261 gcagtaatga aagccgtgag tgagctggat tatcggccaa acgccaatgc ccaggcgctg
    13321 gcaactcagg ttagcgacac cattggcgtg gtggtgatgg acgtttctga tgcgtttttc
    13381 ggcgcgctgg taaaagcggt ggatctagtc gctcagcagc atcagaaata cgtgctaatc
    13441 ggcaatagct atcatgaagc ggaaaaagag cgtcacgcca ttgaggtgtt aattcgccag
    13501 cgttgtaatg cgttgattgt tcactcaaaa gcattgagtg acgatgaact ggcgcaattt
    13561 atggataaca ttcccggtat ggtgttaatc aaccgcgttg tgccggggta cgcccatcgt
    13621 tgcgtttgcc tggataatct cagcggtgcc cgaatggcga cgcgcatgtt gctgaataac
    13681 ggtcatcaac gtattggtta tctttcttcc agtcacggca ttgaagatga cgccatgcgt
    13741 aaagcaggct ggatgagtgc gttgaaagag caggatatta ttccgccgga aagctggatt
    13801 ggcactggta cgccggacat gccgggcggt gaggcggcga tggttgaact gctggggcgc
    13861 aatctacaac ttaccgctat atttgcttat aacgacaata tggccgctgg cgcactgaca
    13921 gcattaaaag ataatggcat tgcgattccg ttacatctct caatcatcgg tttcgatgat
    13981 attcccatcg cccgttacac cgacccgcaa ttaacgaccg tgcgttatcc cattgcttca
    14041 atggctaaat tagccaccga actggccttg cagggggcag caggcaatat tgatcctcgt
    14101 gccagccact gttttatgcc gacgttagtg cgtcgccatt ctgtcgcaac gcgccagaat
    14161 gcggcggcga tcactaactc aacaaatcag gcgatgtaac cgctttcaat ctgtgagtga
    14221 tttcacagaa tcttaacaat gtgatagcta tgattgcacc gttttaacgt tgtaacccgt
    14281 atgtaacagt gaataatcac tttcaccgag gtaatagcgt cataacaaca attaaagccg
    14341 ttttctggag cgttaccggg catggaagaa cgaattttaa aaagtgagct tcggcgttca
    14401 gtaacacttc attaactcta ctgccctgcc gagcatttat ctcaagcact accctgcata
    14461 agaaaaaccg gagataccat gaataagaag gtgttaaccc tgtctgctgt gatggccagc
    14521 atgttattcg gtgccgctgc acacgctgct gatactcgca ttggtgtaac aatctataag
    14581 tacgacgata actttatgtc tgtagtgcgc aaggctattg agcaagatgc gaaagccgcg
    14641 ccagatgttc agctgctgat gaatgattct cagaatgacc agtccaagca gaacgatcag
    14701 atcgacgtat tgctggcgaa aggggtgaag gcactggcaa tcaacctggt tgacccggca
    14761 gctgcgggta cggtgattga gaaagcgcgt gggcaaaacg tgccggtggt tttcttcaac
    14821 aaagaaccgt ctcgtaaggc gctggatagc tacgacaaag cctactacgt tggcactgac
    14881 tccaaagagt ccggcattat tcagggcgat ttgattgcta aacactgggc ggcgaatcag
    14941 ggttgggatc tgaacaaaga cggtcagatt cagtccgtac tgctgaaagg tgaaccgggc
    15001 catccggatg cagaagcacg taccacttac gtgattaaag aattgaacga taaaggcatc
    15061 aaaactgaac agttacagtt agataccgca atgtgggaca ccgctcaggc gaaagataag
    15121 atggacgcct ggctgtctgg cccgaacgcc aacaaaatcg aagtggttat cgccaacaac
    15181 gatgcgatgg caatgggcgc ggtagaagca ctgaaagcac acaacaagtc cagcattccg
    15241 gtgtttggcg tcgatgcgct gccagaagcg ctggcgctgg tgaaatccgg tgcactggcg
    15301 ggcacagtac tgaacgatgc taacaaccag gcgaaagcga cctttgatct ggcgaaaaac
    15361 ctggccgatg gcaaaggtgc ggctgatggc accaactgga aaatcgacaa caaagtggtc
    15421 cgcgtacctt atgttggcgt agataaagac aacctggctg aattcagcaa gaaataagac
    15481 tgattgggcg cagtcgttta ctgcgccctt attgtatggc caacaaggta aaattatatg
    15541 gtcagctcaa cgactccgtc ttccggggaa tacttgttgg aaatgagcgg tatcaacaag
    15601 tcctttcctg gtgttaaggc acttgataac gttaatttaa aagtccggcc acattctatc
    15661 catgcattaa tgggggaaaa cggtgcagga aaatcgacat tattaaaatg cctgtttggt
    15721 atttatcaaa aagactccgg caccatttta ttccagggta aagagatcga tttccattcc
    15781 gccaaagagg cactggaaaa tggtatttcg atggtacacc aggagttaaa cctggtatta
    15841 caacgttcgg tgatggataa catgtggctg gggcgatatc ccaccaaagg catgtttgtc
    15901 gatcaggaca aaatgtaccg cgaaaccaaa gcgatttttg atgaactgga tattgatatc
    15961 gatccgcgtg cgcgcgtcgg cacattatcc gtttcgcaaa tgcagatgat cgaaatcgcc
    16021 aaagcgtttt cctataacgc gaaaattgtg attatggatg aaccgacttc ttcgttaacc
    16081 gaaaaagagg tcaatcatct gttcactatt attcgtaaat taaaagagcg cggctgcggt
    16141 attgtttata tctcgcataa aatggaagaa atcttccagt tatgtgatga agttaccgta
    16201 ttgcgcgacg gtcagtggat cgccaccgaa ccgctggcag gactgacgat ggacaagatc
    16261 atcgccatga tggttgggcg ttctcttaat cagcgttttc ctgacaaaga aaacaagccg
    16321 ggcgaagtca tcctcgaggt gcgtaaccta acgtcactgc gccagccgtc gattcgcgat
    16381 gtctcgtttg atctgcataa aggggagatc ctcggtattg ccgggctggt gggggcgaaa
    16441 cgtaccgata ttgttgagac gttatttggt attcgcgaga aatcggctgg caccattacg
    16501 ttgcacggca aacagatcaa taaccataat gccaacgaag ccataaacca cggatttgca
    16561 ctggtaactg aggagcgccg ctcaacggga atttatgcct atctggatat tggttttaac
    16621 tcgttaattt ccaatattcg caactacaaa aataaagttg gtttactgga taaatcgcgg
    16681 atgaaaagcg atacccagtg ggtgattgat tcgatgcggg taaaaacacc ggggcatcgg
    16741 acgcaaattg gttcgctctc aggtggtaat cagcaaaagg tgattattgg gcgctggcta
    16801 ctaacgcaac cagaaatatt aatgctcgat gaaccgacgc gcggtattga tgtcggggcg
    16861 aagtttgaga tctatcagtt aattgccgaa ctggcgaaga aaggcaaagg gattattatt
    16921 atctcctctg aaatgcctga gttgttaggg ataacagacc gtattctggt catgagcaat
    16981 ggtctcgttt ccggaattgt cgacacaaaa acaacaacgc aaaacgaaat tctgcgtctt
    17041 gcgtctttgc acctttaaga ttaggggctt cccatgagtg cgttaaataa gaaaagtttt
    17101 cttacttacc tgaaagaggg cggtatttac gtcgttcttt tagttttgct ggcgattatt
    17161 attttccagg acccaacatt tttaagtctg ttgaacttaa gtaatattct cacccagtca
    17221 tcggtgcgta ttattatcgc gctcggtgtg gcagggttaa ttgtcaccca ggggaccgac
    17281 ctttctgcgg ggcgtcaagt agggctggca gcagtggtgg cagcgacatt attgcagtcc
    17341 atggataacg ccaataaagt gttcccggaa atggcgacga tgccgattgc gctggttatt
    17401 ctgattgtct gtgccattgg tgcggtgatc ggtttgatca acggtctgat tatcgcttat
    17461 ctcaacgtta cgccgttcat taccacgctc ggcacgatga tcatcgtcta tggcatcaac
    17521 tcgctctatt acgactttgt cggggcgtcg ccaatttctg gttttgacag tggcttctct
    17581 acctttgctc agggctttgt cgcgctgggg agtttccgtc tctcttacat caccttctac
    17641 gcgttgattg cggtggcgtt cgtctgggtg ttgtggaaca aaacccgctt cggtaagaac
    17701 atttttgcca ttggcggtaa cccggaagcg gcaaaagtat ctggtgtcaa cgtcggcctg
    17761 aacctgctga tgatctacgc gttgtctggc gtgttctatg cctttggcgg gatgttagaa
    17821 gccggacgta tcggctctgc caccaacaac ctcggcttta tgtatgagct ggatgctatc
    17881 gcggcgtgcg tggtaggcgg tgtatcgttc agcggcggtg tggggacggt gattggcgtg
    17941 gtgaccgggg taattatttt taccgtcatc aactatggcc tgacgtatat cggcgtaaac
    18001 ccatgctggc agtacatcat caaaggggcg attattatct tcgccgtagc gctggattca
    18061 ctgaaatacg cgcgtaagaa atgatgtgaa tgtggtccgc attcggtcat aaacgcacat
    18121 aacatcatga tctgtgtggt tttgtaggcc ggataaggcg ttcacgccgc atccggcaac
    18181 aggagccaaa tgccggatgc aacgctggcg cgttttatcc ggcctacaat aagctcaaca
    18241 tactgatttg tatggttttg taggccggat aaggcgttca agccgcatcc ggcgacagga
    18301 accaaatgcc ggatgtgacg cgggttctac aacgttaccg ggtgttcttt ctcgcctttc
    18361 ttaaacttca cttccccgag ctcaatacaa cccaccgggc agacgtgacc acaaagcaga
    18421 caacccacac atttctcggt attacaatgc ggtgtgcggg ttttctcgct ccattccatc
    18481 gcctggtgac cgccgtcgta acaggaaata taacagcgtc cacagccaac acatttgtca
    18541 agattgatac ggggatagac aatataactg cggtctaaat cttccgccgg gacaatattg
    18601 ttattcgcca gacctaccat ctcctgcaac gaatcaaatc cctgatcggc gagataatgc
    18661 gataacccgc tcgccatatc ttctacgatg cgattgccgt actgcataat gccggtggtg
    18721 acctgtaacg ttgctgcgcc gagcaataaa aactccgccg catcctccca ggtttcaatg
    18781 ccgccgatac cgctgattgg gaaatcgcgc agttctggat gggtgcgcat ttgctgaatg
    18841 aagcgcaggg cgatcggttt taccgcttta ccggaatatc cggaaatact cgatttaccg
    18901 ttaacgatcg gcataccgat tttctgatta
//
LOCUS       DAJTOR010000073        18506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTOR010000073 DAJTOR010000000
VERSION     DAJTOR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.073"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..893
                     /locus_tag="OO249_002202"
     CDS             <1..893
                     /locus_tag="OO249_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5636492.1"
                     /translation="WFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFIT
                     IGVLGIFLAIGWYMLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTM
                     WGMMLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMTAIPFLFGAAGMLVNGYVT
                     DWLVKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTS
                     CRGLIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTA
                     AGALAYIFLVRQPINDPRKD"
     gene            complement(942..2597)
                     /gene="tsr"
                     /locus_tag="OO249_002203"
     CDS             complement(942..2597)
                     /gene="tsr"
                     /locus_tag="OO249_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5636493.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2716..3162)
                     /gene="hpaR"
                     /locus_tag="OO249_002204"
     CDS             complement(2716..3162)
                     /gene="hpaR"
                     /locus_tag="OO249_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5636494.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3434..4723
                     /locus_tag="OO249_002205"
     CDS             3434..4723
                     /locus_tag="OO249_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5636495.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            4720..6186
                     /gene="hpaE"
                     /locus_tag="OO249_002206"
     CDS             4720..6186
                     /gene="hpaE"
                     /locus_tag="OO249_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5636496.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRDHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6188..7039
                     /gene="hpaD"
                     /locus_tag="OO249_002207"
     CDS             6188..7039
                     /gene="hpaD"
                     /locus_tag="OO249_002207"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5636497.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7049..7429
                     /locus_tag="OO249_002208"
     CDS             7049..7429
                     /locus_tag="OO249_002208"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5636498.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            7540..8343
                     /gene="hpaH"
                     /locus_tag="OO249_002209"
     CDS             7540..8343
                     /gene="hpaH"
                     /locus_tag="OO249_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5636499.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8354..9142
                     /gene="hpaI"
                     /locus_tag="OO249_002210"
     CDS             8354..9142
                     /gene="hpaI"
                     /locus_tag="OO249_002210"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5636500.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9317..10693
                     /gene="hpaX"
                     /locus_tag="OO249_002211"
     CDS             9317..10693
                     /gene="hpaX"
                     /locus_tag="OO249_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5636501.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10703..11589
                     /gene="hpaA"
                     /locus_tag="OO249_002212"
                     /pseudo
     CDS             10703..11589
                     /gene="hpaA"
                     /locus_tag="OO249_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            11840..13402
                     /gene="hpaB"
                     /locus_tag="OO249_002213"
     CDS             11840..13402
                     /gene="hpaB"
                     /locus_tag="OO249_002213"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR5636502.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSANDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13420..13932
                     /locus_tag="OO249_002214"
     CDS             13420..13932
                     /locus_tag="OO249_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5636503.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14325..16475
                     /gene="btsT"
                     /locus_tag="OO249_002215"
     CDS             14325..16475
                     /gene="btsT"
                     /locus_tag="OO249_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5636504.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16606..16809
                     /locus_tag="OO249_002216"
     CDS             16606..16809
                     /locus_tag="OO249_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5636505.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16820..17776
                     /gene="yjiA"
                     /locus_tag="OO249_002217"
     CDS             16820..17776
                     /gene="yjiA"
                     /locus_tag="OO249_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5636506.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(17878..>18506)
                     /locus_tag="OO249_002218"
     CDS             complement(17878..>18506)
                     /locus_tag="OO249_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR5636507.1"
                     /translation="PGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTG
                     SLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVS
                     NWKTPAMALTFRANNFFDVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWD
                     VVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWLTKQDVEAHKES"
BASE COUNT         4227 a         5124 c         4938 g         4217 t
ORIGIN      
        1 actggttcaa catcaaagag cgcggacgcc cgatgggctt cttcaacgca gcttctacca
       61 ttggcgttgc cgtaagccca ccgattctgg cggcgatgat gctggtgatg ggctggcgcg
      121 ggatgtttat taccattggt gtactgggga tttttctcgc catcggctgg tatatgctct
      181 atcgcaaccg cgagcacgta gaactgactg ccgttgagca agcttatctc aatgcaggta
      241 gcgtcaatgc ccgccgagat ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc
      301 gtacaatgtg gggaatgatg ctcggattca gtggcatcaa ctacactgcg tggctgtatc
      361 tggcctggct tcctggttac ctgcaaacag cctataacct ggatttaaaa agcacagggt
      421 tgatgacggc tatccctttc ctgtttgggg ctgccgggat gctggtcaac ggttacgtta
      481 ccgactggct ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc tgcattattg
      541 ccgggatgtt ctgttctgcc gcctttacgc tgatagtacc acaagcgaca acatccatga
      601 cggcggttct gctgattggc atggcactgt tctgtattca ctttgccgga acatcctgcc
      661 ggggcttgat ccacgtcgca gttgcttctc gcatgactgc gtcggtgggc agtatccaga
      721 actttgccag cttcatctgc gcctcttttg cgccgatcat tactggtttt attgttgata
      781 ccacccactc attccgtctg gcactaatca tctgcggttg cgtcaccgca gcgggggcac
      841 tggcgtacat cttcctggtt cgtcagccga tcaacgaccc acgtaaagat taatctcctt
      901 atgcccgata acattttgct tatcgggcat tttcatggcg attaaaatgt ttcccagttc
      961 tcctcgctat ctgccacggc cattttacgc ggcgcagctg gcgtcacggt ttttaccaca
     1021 gccgatgttt cacgctgctg ttgctgaatc cggaacactg ccactgcttc ggtcaggcga
     1081 ctggcctgct cttccagcgc ggcggcggca gcggcagact cttccaccag cgcggcgttc
     1141 tgttgagtta cccggtccat ctcagcaacc gctaagccaa cctgatcgat accacggctc
     1201 tgctcatcag aagcagaagc aatttcgccc ataatgtccg tcacgcgggt cacggcgctg
     1261 acaatctccg ccattgtttc cccggcgctt tcgaccagcg tagagccaac atccactttc
     1321 cccaccgagt cttcaatcag gcttttaatt tcacgagccg cctgggcgct gcgctgggcc
     1381 agattacgca cttctcccgc gaccaccgca aaaccgcgcc cttgctcacc cgcacgcgca
     1441 gcctcaaccg ccgcgttcaa agccagaata ttggtctgga aggcaatgcc gtcaattacg
     1501 ctgataatat cggcgatttt ctgcgaactg gtggagatat cgcgcatagt ctgcaccacg
     1561 ttatctacca ctttgccgcc gcgttgcgcc gtttcagaag cacttaacgc cagatggctg
     1621 gcctggcgcg cattttcggc gttctgcttc accgttgcgg tcagttgctc catgctggct
     1681 gccgtctctt ccagcgaagc ggcctgttgc tcggtgcgcg aagagagatc gttattgccg
     1741 gtggcgattt cgctggcacc gctatagatg gcattggccc cgttgcgcac atcaccgacg
     1801 gtacgcatca gctctccctg catatggcgc aaactctctg ccagttgccc catctcatta
     1861 gagccatcca cctcaatcgg tttcaccaga tcgccgcctg caatatgacg aatgctgtca
     1921 atcaggcgat tcattggcgc taccagcgag gctttaatac cgaaccagac ggcgaagatg
     1981 accgccagta cgacgatcat cacgcccacc agaatccaca tcgcctggct gtaggaggca
     2041 ttgttatcgc tgacggcgat atcatagagc cgatcgtttt gctccatgta agccacatac
     2101 tgcttctcga aaccgtcctg atatccctgg gtcggctgat caaagaactc gttgattttg
     2161 cctgcaccta acagttggat cagctccgcc agcgcattgt gataaatatc gtaattacgt
     2221 ttgatctctg ccgctgcggc ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatcc
     2281 gcccagtttt tttccgcctg tttcagcgaa atactggcac tctgcatcag ctcagcaacg
     2341 gttgaaccgc taccaatatt attctgatcc atcatgtagc ggatacccgc gcggttgagg
     2401 gtgttacgcg tctgcaacaa cgcgacccag ctgccattca gcgtggattg ctgctggcga
     2461 atggtttgta aaacagtgaa attttctttg tcattcttta aggcattaaa gaacagaccg
     2521 cctgatgtca gttgtaaaag gccaaaaacg gccaaaacca gcagtaagct ggtcacgatc
     2581 ttgatacgtt ttaacatggt ttctctttcc tgtggaccga tgcaagattt tcggcctgga
     2641 aagcaaaaac tttatgaaaa ggtgtaaccc gccatccatg gaaaaaccag gcgctggtta
     2701 tcagatacta aaaagttatt cattatcgcc cgggatggct tcttgtcggg aattgcccag
     2761 agcaataaat tcctctaaca gatgggttaa ctgttgcatt ttctcggcag taaactgagc
     2821 ttcaatctgc cgataggctt cttcaatctg tgtctgagcg cggttataca gcgcctgccc
     2881 ctctttggtt agcgagatat agagcttccg ctgatcattg atcggcttta atcgcaacac
     2941 taaaccgtcg cgttccatcc gcgtgagaat cccggtcagg cttgggcgca aaatgcaggc
     3001 gcgatacgcc agatcgtgaa aatccattga tgggctttcc gccagaatac gcacgatgcg
     3061 ccactgttgc tcagtcaaat tatgccgttt aacgattggg cgaaaataac tcatcgccgc
     3121 ttctcgcgcc tggagcaacg caatggttag tgagtcgtgc attatctttc ccctgaaaaa
     3181 ttcaaaatca ttaatataga aacagttaat cgtcacgtta ccgggactgc gctgcccctg
     3241 caattcctga ttttatcaaa attgatgata ttttattaat atactgatat ttaatatttt
     3301 attatcttta aatgtaaata gtttgttaat tagatcacat ttacatcact tacttttgcc
     3361 aaactacgca atcgggaata aaagcaatca ttaacatatt aatgattaag ataatcgaat
     3421 ccgaggagtg gtaatgaaag gcactatctt cgccgtagcg ttgaaccatc gcagccaact
     3481 tgatgcatgg caggaagcgt tccagcaatc cccctacaaa gccccgccta aaactgcggt
     3541 ctggtttatt aaaccgcgca atacggtgat tggttgcggt gaaccgattc cctttccaca
     3601 gggtgaaaag gtactgagcg gtgcgactgt tgcgctgatt gtgggaaaaa cggcgacgaa
     3661 agtacgtgaa gaagatgcgg cagagtacat cgccggatat gcgctggcta acgacgtcag
     3721 cctgccggaa gagagctttt accgcccggc aatcaaagca aaatgtcgtg atggattctg
     3781 ccccattggc gaaaccgtgg ctctcagcaa tgtcgataat ctgaccatct ataccgagat
     3841 caacgggcgt cctgccgatc actggaatac cgccgattta caacgtaacg ccgcgcagtt
     3901 gctgagcgcc ctgagcgaat ttgccacgct gaatccaggc gacgccattc tgctcggcac
     3961 gccacaggcg cgcgtggaaa tacagccagg cgatcgcgtt cgtgttctcg cagaaggttt
     4021 cccgccgctg gaaaatccgg tagtggacga acgtgaagtg accacgcgca agagcttccc
     4081 aacgcagcca cacccgcacg gcacgctgtt tgccctcggc ctgaactacg ccgaccacgc
     4141 cagcgaactg gaatttaagc caccggaaga accgctggta ttcctgaaag cgccaaatac
     4201 cctcactggc gataaccaga cctccgtgcg tccaaacaat attgaataca tgcactatga
     4261 agcggagctg gtggtagtta ttggcaaaca ggcgcgtaac gtcagcgaag ccgatgccat
     4321 ggattatgtc gcgggctaca ccgtgtgtaa cgactacgcc attcgtgact atctggaaaa
     4381 ctactaccgc cctaacctgc gggtaaaaag ccgcgacgga ctgacgccga tgctttcaac
     4441 catcgtgccg aaagaggcga tcccggaccc gcataatctg acccttcgca ccttcgtcaa
     4501 cggcgagtta cgccagcaag gcaccaccgc cgatctgatc ttcagcgtgc ccttcctgat
     4561 cgcctactta agtgaattta tgaccctgaa tccgggcgac atgatcgcca ccggcacacc
     4621 caaaggctta tctgacgtgg tgcctggcga tgaagtagtg gtggaagtag aaggcgtggg
     4681 ccgcctggtg aaccgaattg tgagtgagga tacagcgaaa tgaaaaaagt aaatcattgg
     4741 atcaacggca aaaatgttgc aggtaacgac tacttccaga ccaccaatcc ggcaacgggt
     4801 gaagtgctgg cggatgtggc ctctggcggt gaagcggaga tcaatcaggc ggtagcggca
     4861 gcgaaagagg cgttcccgaa atgggccaat ctgccgatga aagagcgtgc gcgcctgatg
     4921 cgccgtctgg gcgatctgat cgaccagaac gtgccagaga tcgccgcgat ggaaaccgcg
     4981 gacaccggcc tgccgatcca tcagaccaaa aatgtgttga tcccacgcgc ttcccacaac
     5041 tttgaatttt tcgcggaagt ctgccagcag atgaacggca agacctatcc ggttgacgac
     5101 aagatgctca actacacgct ggtgcagccg gtgggcgttt gtgcgctggt atcgccgtgg
     5161 aacgtaccgt ttatgaccgc cacatggaag gtcgcgccgt gtctggcgct gggcaatacc
     5221 gcggtactga aaatgtcgga actctccccg ctgaccgctg accgcctggg tgagctggcg
     5281 ctggaagccg gtattccggc aggcgtgctg aacgtggtac agggctacgg cgcaaccgca
     5341 ggggatgcgc tggttcgtga tcatgacgta cgtgccgtgt cgttcaccgg cggtacggcc
     5401 accgggcgca acatcatgaa aaacgccggg ctgaaaaaat actccatgga actgggcggt
     5461 aaatcgccgg tgctgatttt tgaagatgcc gatattgaac gcgcgctgga cgccgccctg
     5521 ttcaccatct tctcgatcaa cggcgaacgc tgcaccgccg gttcacgcat ctttattcag
     5581 caaagcatct acccggaatt cgttaaacgc tttgccgaac gcgccaaccg tctgcgcgtg
     5641 ggcgatccga acgatccgaa tacccaggtt ggcgcgctta tcagccagca gcactgggaa
     5701 aaagtctccg gctatatccg tctcggcatt gaagaaggcg ccaccctgct ggcgggcggc
     5761 ccggataaac cgtctgacct gcctgcacac ctgaaaggcg gcaacttcct gcgcccaacg
     5821 gtgctggcag acgttgataa ccgtatgcga gtcgcccaag aagagatttt cgggccggtc
     5881 gcctgcctgc tgccgtttaa agacgaagct gaaggcttac gcctggcaaa cgacgtggag
     5941 tacggcctcg cgtcgtacat ctggacacag gatgtcagca aagtgttacg cctggcgcgt
     6001 ggcattgaag ctggcatggt gttcgtcaac acccagaacg tgcgtgacct gcgccagcca
     6061 tttggcggcg taaaagcctc cggcaccggg cgtgaaggcg gtgagtacag cttcgaagtg
     6121 ttcgcggaaa tgaagaacgt ctgcatttcc atgggcgacc atccaattcc gaaatgggga
     6181 gtctgatatg ggtaagttag cgttagccgc caagatcacg cacgtaccgt cgatgtatct
     6241 ctctgaactg ccagggaaaa accacggttg ccgccagggc gcgatcgacg ggcataaaga
     6301 gatcagcaag cgttgccggg aaatgggcgt cgataccatt atcgttttcg atacccactg
     6361 gctggtcaac agtgcttatc acatcaactg tgcagaccat tttgaaggcg tctacaccag
     6421 caacgagctg ccgcatttta tccgcgatat gacctacaac tacgagggca acccggagtt
     6481 ggggcagctt attgccgatg aagccttaaa gctcggcgtg cgggcaaaag cgcacaacat
     6541 tcccagcctg aaactggaat acggcacgct ggtgccgatg cgctacatga atgaagacaa
     6601 gcacttcaaa gtggtctcca tttcggcttt ctgcacggtt cacgattttg ccgacagccg
     6661 caagctgggc gaagcgattc tgaaagcgat cgaacagtac gacggcaccg tggcggtcct
     6721 tgccagcggt tcgttatcgc accgctttat tgacgatcag cgtgcagaag aagggatgaa
     6781 cagctacacc cgcgagttcg accgccagat ggacgagcgc gtggtgaagt tgtggcgcga
     6841 aggccagttc aaagagttct gcaatatgct gccggagtac gccgactact gctacggcga
     6901 aggcaatatg cacgacacgg tgatgctgct ggggatgctc ggctgggata aatacgacgg
     6961 caaggtggag tttatcaccg agctattccc aagctctggc accggtcagg ttaacgctgt
     7021 tttcccgctt cccgcgtaag gacgttttat gccgcacttt atcgttgaat gcagtgacaa
     7081 catccgcgaa gaagccgacc tgccggggtt gttcgccaaa gtgaatccga cgctggcagc
     7141 cacgggtatt tttccgctgg cgggtattcg cagccgcgtg cattgggtcg atacctggca
     7201 gatggccgac gggcagcgtg attatgcctt cgtgcatatg acgttgaaaa tcggcgcagg
     7261 tcgcagcctg gaaagccgtc agcaggcggg tgaaatgctg tttgaactga ttaaaacgca
     7321 cttcgccgcc ctgatggaga gccgcctgct ggcgttgtcg tttgagattg aagagctgca
     7381 tccgacgctg aattttaaac aaaacaacgt gcacgcattg tttaagtagc gcgcagattg
     7441 cccggtggcg ctgcgcttac cgggcctaca aaaccccaaa ccgtacaccg taggccggat
     7501 aaggcgcagc cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac
     7561 accctgatcg cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc
     7621 tcgctggatt acccagagat caccatcgaa gacgcttacg cggtgcagcg cgaatgggtt
     7681 cgcctgaaaa tcgccgaagg ccgtacgctg aaaggccaca aaatcggtct gacctcaaaa
     7741 gcaatgcagg ccagctcgca gatcagcgaa ccagattacg gtgccctgct ggatgacatg
     7801 ttcttccacg atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg
     7861 gagctggctt tcgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtc
     7921 tacaacgcca cggactacgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac
     7981 atcgatccgg aaacccagcg cccgcgcaaa gtgttcgaca ccatttctga taacgccgcc
     8041 aacgccgggg tgatcctcgg tggtcgtccg atcaaaccag acgaactgga tctgcgttgg
     8101 atctccgccc tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggcgtg
     8161 ctgaatcatc cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta
     8221 caactggaag ccgggcaaat cattctcggt ggttcgttca cccgcccggt tccggcgcgt
     8281 aagggcgaca ccttccacgt cgattacggc aacatgggtt ccattagctg ccgctttgtt
     8341 taaggagaga acgatggaaa acagctttaa agcggcgctg aaagcaggcc gtccgcagat
     8401 cggattatgg ctggggctga gtagcagcta cagcgcagag ttactggccg gagcaggatt
     8461 cgactggttg ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca
     8521 gctacaggcg attgcgccct accccagcca gccggtagta cgtccgtcgt ggaacgatcc
     8581 ggtgcaaatc aaacaactgc tggacgtcgg cacacaaacc ttgctggtgc cgatggtaca
     8641 aaacgctgac gaagcccgtg aagcggtacg cgccacccgt tatccccccg ccggtattcg
     8701 cggtgtgggc agtgcgctgg cccgcgcctc gcgctggaat cgtattcctg attacctgca
     8761 aaaagccaac gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa
     8821 cttaccgcag attctggacg tggaaggcgt cgacggcgtg tttatcggcc cggcggatct
     8881 gagcgccgat atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga
     8941 gcaggcgatc gtgcagatcc gtgaatcggg caaagcgccg gggatcctga tcgccaatga
     9001 gcaactggca aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac
     9061 caccctgctc gcccgcgccg ctgaagcgct ggcagcacgg tttggtgcgc aggccaccgc
     9121 cgtgaagccc ggcgtgtatt aatgccggat gcgctgcact tatccggcct acactcgcac
     9181 caaaccgtag gccggataag gcgtttacgc cgcatccggc aaaaagccgt accaaatcgc
     9241 ggaccggata aggcgttacg ccgcatccgc cataaacctt gtcgtaccct acaaaaatcc
     9301 cattagagga agaaaaatga gcgacacctc acctgccata ccggagagta tcgatccggc
     9361 gaatcagcat aaagcgctga ctgccggaca acaggcggtt attaagaagc tatttcgccg
     9421 cctgatcgtc tttctgttcg tgctgtttat cttctcgttc cttgatcgca tcaacatcgg
     9481 ctttgccgga ctcacgatgg gacgcgacct cggtctgagc gccaccatgt ttggcctcgc
     9541 taccaccctg ttctatgccg cttatgtcat cttcggcatt cccagcaaca ttatgctgag
     9601 tattgtcggt gcgcggcgct ggatcgccac catcatggtg ctctggggca tcgcctctac
     9661 tgccaccatg tttgccactg gccccaccag cttgtacgta ctgcgtatac tggttggcat
     9721 taccgaagcc ggctttctgc ctggtattct gctgtattta accttctggt ttccggccta
     9781 tttccgcgcc cgtgccaacg ccttgtttat ggtagcaatg ccggtaacga cagcgttggg
     9841 ctcgatcgtt tccggctaca ttttgtcgct ggatggcgtc atggcattaa aaggctggca
     9901 gtggctgttt ttgctggaag gcttcccgtc ggtattactc ggcgtcatgg tgtggttctg
     9961 gcttgatgac tcaccggaca aagctaagtg gctgacgaaa gaagacaaaa aatgcctgca
    10021 agagatgatg gataacgatc gtctgacgct ggttcagcca gagggagcca tcagccacca
    10081 cgccatgcaa caacgcagta tgtggcggga gatcttcact ccggtggtga tgatgtatac
    10141 cctggcgtat ttctgcctga ccaacacact tagtgcgatc agcatctgga caccgcagat
    10201 cctgcaaagt tttaatcagg gcagcagtaa tatcaccatc ggcctgctgg ccgccgtacc
    10261 gcagatttgt accattctcg ggatggtcta ctggagccgt cactcagatc gccgccagga
    10321 acgaaggcat cacaccgccc ttccttattt gttcgctgcc gcaggttggt tactggcttc
    10381 ggcaactgat cacaacatga tccagatgct ggggatcatt atggcttcga ccggatcatt
    10441 cagcgcaatg gcgattttct ggacaacacc ggatcagtcc atcagcctgc gggcacgagc
    10501 gatcggtatt gcggtgatca acgccactgg caacattggt tcagcgttaa gtccgtttat
    10561 gatcggctgg ttgaaagatt tgaccggcag ctttaacagt ggattgtggt ttgttgccgc
    10621 gctgctggtg attggtgcgg ggattatctg ggcgattcca atgcagtcct cccgtccgcg
    10681 agcgaccccg taaggaacga cgatgtgtga ccgtcagatt gccaatattg atatcagcaa
    10741 agagtacgat gaaagcctgg gcacggacga tgtgcattat cagtcgttcg cccgcatggc
    10801 ggcatttttt ggccgccata tgctgccaca tcgccacgaa cagtactttc agatgcattt
    10861 cctcaatagc ggacagattg agctacagct tgacgatcat cgctactcgg tggaagcgcc
    10921 cctgtttgtc ctgacgccgc cgtcagtacc tcatgcgttt attacggagt ctgacgccga
    10981 cggtcatgtg ttgacggtac gggaagatct gatctggccc ctgctggaag ttctttatcc
    11041 gggcactcgg gaaaccttcg gcctgccggg ggcctgtcac tggcagataa acccgacgaa
    11101 ctggcggcgc tggaacacta ttggcaactg atagagcggg aatcggtgga acaactgcct
    11161 ggacgggaac acaccctgac gttactggca caggcagtgt tcaccctact gctgcgtaac
    11221 gcaaaactcg acgaccatgc cgccagcggg atgcgcggag aattaaaact gttccagcgt
    11281 tttaatatgc tgattgaaag ccattttcat cagcactgga cagtaccgga ttacgctaac
    11341 gaactgcata tcaccgaatc acgactcacg gacatctgcc gtcgctttgc caaccgtccg
    11401 ccaaaacggt tgattttcga caggcagctg cgagaagcca agcggctgct gctgttttct
    11461 gataacgccg tgaacaatat tgcctggcaa ctcggtttta aggatccggc ttattttgcg
    11521 cgctttttta atcgcttagt cggttgctcg cccagtgctt atcgcgccaa aaaagtacct
    11581 gtgacgtgaa aatcccttag ccttaacggg aaaccaggca ccacctgcta ttcccctttc
    11641 tccgcctgga gcaaggggat ttagtaacgt caaaaatcgc taaaagcgac ctcgatcaca
    11701 aaaccgtacc cgacctgaaa agtaccagcc attgtcccaa aagtctcttc ccgtaatcgc
    11761 ccctgcaagc tttaattgaa taacaaaaac aacacacaaa attaacaatc gtattccgat
    11821 taatactgta gaggtcgaca tgaaaccaga agatttccgc gccagtaccc aacgtccgtt
    11881 caccggggaa gagtatctga aaagcctgca ggatggtcgc gagatctata tctatggcga
    11941 gcgagtgaaa gacgtcacta ctcatccggc atttcgtaat gcggcagcgt ctgttgccca
    12001 actgtacgac gcgctacaca aaccggagat gcaggactct ctgtgctgga acaccgacac
    12061 cggcagcggc ggctataccc ataaattctt ccgcgtggcg aaaagtgcca acgacctgcg
    12121 ccagcaacgc gatgccatcg ctgagtggtc acgcctgagc tatggctgga tgggccgtac
    12181 cccagactac aaagctgctt tcggttgcgc actgggcgcg aatccgggct tttacggtca
    12241 gttcgagcag aacgcccgta actggtacac ccgtattcag gaaactggcc tctactttaa
    12301 ccacgcgatt gttaacccac cgatcgatcg tcatttgccg accgataaag taaaagacgt
    12361 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    12421 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    12481 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtaaa
    12541 attaatctct cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    12601 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtgctgat
    12661 cccatgggaa aacgtactga tctaccgcga tttcgatcgc tgccgtcgct ggacaatgga
    12721 aggcggtttt gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaattaga
    12781 cttcattaca gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    12841 tgtacaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    12901 ctcgatgtgt tctgaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    12961 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattatcga
    13021 acgcaacgtt accagtggcc tgatctatct cccttccagt gcccgtgacc tgaacaatcc
    13081 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aatggtatgg atcacgtcca
    13141 gcacatcaag atcctcaaac tgatgtggga cgccattggc agcgagtttg gtggtcgcca
    13201 cgaactgtat gaaatcaact actccggtag ccaggatgag attcgcctgc agtgtctgcg
    13261 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    13321 ggaatacgac cagaacggct ggactgtgcc gcacttgcac aacaacgacg atatcaacat
    13381 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacagcgc
    13441 ctgcgctttc gtgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    13501 ggcgacgccg gacaatgcgg gattacggca acggccgtct gctcggtcac ggatacacca
    13561 ccatcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    13621 ggtaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg ccacttcgcg
    13681 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    13741 ctggcgcagc cggtgctgaa aggttcgctg gccagtcttg aaggtgagat ccgcgatgtg
    13801 caggcaattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    13861 gaaggtcacg gacttatcta ctttaaacgc cgtttccatc cagtgatgct ggaaatggaa
    13921 gctgcgattt aagctccagc cccttcacgt attcgtcgaa ggggcatttt tattgtgatc
    13981 tcaatcacat atccacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    14041 gcttacgtcc ctaaaataac cactcagtta tttaccttac tttacgcgcg cgtaactctg
    14101 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    14161 ggcttcggcc tatgattaac aaattactct atgaattttg gtttgcagga aggcagcaag
    14221 tgagtgaatc cccgggagct tacaacagta agtgacagag gtgaacgaac gcagctgccg
    14281 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    14341 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    14401 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    14461 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    14521 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    14581 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgccggt ccgctggttg
    14641 gtccggtgct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    14701 tagtactggc cggtgcggtt caggacttta tggtgctgtt tatctcctct cgccgtaacg
    14761 gcgcatctct cggtgagatg atcaaagaag agatgggacc agtaccgggg actatcgcgc
    14821 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    14881 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    14941 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtaggtgaa gtttctgtta
    15001 ttggtatcgt gctgctggtt gcctctatct atttcggtgg cgtgattgct cacgatccgt
    15061 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    15121 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    15181 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggta ctgaacccgg
    15241 aactgaaaat gcctgcaatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    15301 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    15361 tgatctcttc cggtacgacg ccgaaattgc tggctaacga aaccgacgcg cgtttcattg
    15421 gctacggcgc aatgctgatg gaatccttcg tggcgattat ggcgctggtt gctgcgtcca
    15481 tcatcgaacc gggtctttac ttcgcgatga ataccccgcc tgctggcctt ggcatcacca
    15541 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    15601 acgttaccgc acacgcggcg gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    15661 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggcg
    15721 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    15781 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    15841 atgcgggtac tcgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    15901 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    15961 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    16021 ggccgctgtt cggtatttcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    16081 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    16141 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    16201 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    16261 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    16321 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    16381 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aactccgtac gttccaatcc
    16441 cggaaggcgg cgtgaagatc tcttcgcacc actaaccggt tgccggatgc gctgcgcgta
    16501 tccggcctac aaagtgtcta tcgtaggccg gataaggcgt ttacgccgca tccggcaaat
    16561 ccctagcccg gaccttccgg gctttacgca tatctggaag gcaaaatgtt tggtaactta
    16621 ggacaggcaa aaaaatatct cggccaggcg gcgaagatgt tgattggcat tccagactac
    16681 gacaactacg tcgagcatat gaagaccaac catcccgaca agccgtacat gagctatgaa
    16741 gaattcttcc gcgaacgcca gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc
    16801 tgttgttaat ggagagagca tgaacccgat tgcagttacc ctactcaccg gttttttagg
    16861 cgcgggcaaa accaccctgc tgcgccatat tcttaacgaa caacacggct acaagattgc
    16921 cgtgattgaa aacgaattcg gcgaagtctc cgttgatgat caactgattg gcgaccgcgc
    16981 cacgcagatc aaaacgctga ccaacggctg catctgctgt tcgcgctcca acgagctgga
    17041 agacgcacta ctcgacctgc tggataatct cgacaagggc aatattcagt tcgaccgtct
    17101 ggtcattgaa tgcaccggca tggccgatcc cggcccgatt attcagacct tcttctccca
    17161 tgagatttta tgccagcgct acctgctgga cggggtgatt gcgctggtgg atgcggtaca
    17221 tgccgatgag cagatgaacc agttcaccat cgcccagtcc caggttggct acgccgaccg
    17281 tattctgctg acgaaaaccg acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc
    17341 gcgcatcaac gcccgcgcac cggtctacac cgttacccac ggcgatatcg acctgggcct
    17401 tctgttcaac accaacggtt ttatgctgga agaaaacgtc gtcagcacca aaccgcgttt
    17461 ccactttatc gcggacaaac aaaacgatat ttcgtcgatt gtggtggaac tggattaccc
    17521 ggtagatatc agcgaagttt cccgggtgat ggaaaacctg ctgctggaat cggcggataa
    17581 actgctgcgt tacaaaggga tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca
    17641 gggcgtccag cgcctctaca gcgccgactg ggaccgcccg tggggcgatg aaaaaccgca
    17701 tagcacgatg gtgtttatcg ggattcagtt gccggaggat gagattcggg cggcgtttgc
    17761 gggactcaaa aagtaaccag ccctccaaca tcacaatacc tgagacttta tcaccctcat
    17821 attcctccag aatatgaggg cattcgggct taattatctc tgaacgtgca acacttccta
    17881 cgactcctta tgcgcctcca catcctgctt cgttaaccag agcgacttac gaccaaaatt
    17941 ttcgtacatt ttcagctgat cttcatagtg ctgatcaact gttccatcgg gagcaataaa
    18001 cccactctga ccgggtgcga ccacatccca ggcaagcacc ggacgatcgc ttgtcgttgg
    18061 tgagaaaaca atcatatcgt tttctgttcc acggttttga tactccgcct gatgacgcgt
    18121 ttcttccgct gcggcctgcg gtacatcaaa gaaattattt gcccggaacg ttaaggccat
    18181 tgcaggtgtt ttccagttac tcacattatt gccatagcgt ttggaaagag tctcccaggt
    18241 atcttccagc gcagccaaca caacctcctg ctgtggtttc ccagcaaaca gatcaaccgc
    18301 ctgtgggatt ggtgatttgt ctccctgcac cgcctcatac aaaatttttg ctccaacact
    18361 tatattcagc gaaccagttg ggccgtcctg ggttgtttcg tagccactgg cgctgtacca
    18421 cttatcaaat ggcataggta cggcagccac tacggtacgc ttcaacatac tggtcagcca
    18481 aacgttcagg atggcagagc ctggct
//
LOCUS       DAJTOR010000074        18424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTOR010000074 DAJTOR010000000
VERSION     DAJTOR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.074"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..179
                     /locus_tag="OO249_002219"
     CDS             <1..179
                     /locus_tag="OO249_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636508.1"
                     /translation="VTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLI
                     DTVEVKANKAGAAA"
     gene            319..501
                     /locus_tag="OO249_002220"
     CDS             319..501
                     /locus_tag="OO249_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCR5636509.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            425..772
                     /locus_tag="OO249_002221"
     CDS             425..772
                     /locus_tag="OO249_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR5636510.1"
                     /translation="MIKGPLRKSAPVSQPEANPKNPLLLNNPFQRENPMSEITSLVTA
                     EAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDAILDELLGVAAVPEPEGIAGDGSAS
                     DGGEPTPDSDMMM"
     gene            849..1064
                     /locus_tag="OO249_002222"
     CDS             849..1064
                     /locus_tag="OO249_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5636511.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1069..1419
                     /locus_tag="OO249_002223"
     CDS             1069..1419
                     /locus_tag="OO249_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR5636512.1"
                     /translation="MTQNYKLIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQNVWITYKGKLPG
                     RITGSLKTPPESQP"
     gene            1483..2016
                     /locus_tag="OO249_002224"
     CDS             1483..2016
                     /locus_tag="OO249_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5636513.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLSEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            2315..2752
                     /locus_tag="OO249_002225"
     CDS             2315..2752
                     /locus_tag="OO249_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5636514.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKEQRDKATSTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGM
                     GNGESPRLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            3169..3789
                     /locus_tag="OO249_002226"
     CDS             3169..3789
                     /locus_tag="OO249_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCR5636515.1"
                     /translation="MKDQDVRFAVHHKLLKESHLDPDCLVVDEFSISLGASRADIAVI
                     NGVIHGYELKSEYDSLERLPLQIKHYSSVMDKVTLVVAEKHLEGALKLIPGWWGVKTV
                     SVGPKGAILIKHMRGEKLNRNHDTLMLAQLLWKDECIDVLERWGYSKGIKSKPRFELW
                     NIIAENIPIANLRLEVRTALKKRVGWKVKAWQAESAPTNKAVSRLT"
     gene            complement(3731..4798)
                     /locus_tag="OO249_002227"
                     /pseudo
     CDS             complement(3731..4798)
                     /locus_tag="OO249_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
     gene            5211..5756
                     /locus_tag="OO249_002228"
     CDS             5211..5756
                     /locus_tag="OO249_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR5636516.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            5731..7656
                     /locus_tag="OO249_002229"
     CDS             5731..7656
                     /locus_tag="OO249_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5636517.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            7653..7859
                     /locus_tag="OO249_002230"
     CDS             7653..7859
                     /locus_tag="OO249_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCR5636518.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            7856..9457
                     /locus_tag="OO249_002231"
     CDS             7856..9457
                     /locus_tag="OO249_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5636519.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            9438..10757
                     /locus_tag="OO249_002232"
     CDS             9438..10757
                     /locus_tag="OO249_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5636520.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            10767..11099
                     /locus_tag="OO249_002233"
     CDS             10767..11099
                     /locus_tag="OO249_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5636521.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDSAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            11155..12180
                     /locus_tag="OO249_002234"
     CDS             11155..12180
                     /locus_tag="OO249_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5636522.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGSTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            12222..12617
                     /locus_tag="OO249_002235"
     CDS             12222..12617
                     /locus_tag="OO249_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5636523.1"
                     /translation="MTKDELIVRLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTPLRPENALTGHENEVVSAQTDTVTDAAALVTVVALVTLHTDALHATRDEP
                     VAFVLPETAFRVSAGVAAEMTERGLARMQ"
     gene            12629..12982
                     /locus_tag="OO249_002236"
     CDS             12629..12982
                     /locus_tag="OO249_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR5636524.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            12994..13572
                     /locus_tag="OO249_002237"
     CDS             12994..13572
                     /locus_tag="OO249_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5636525.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            13569..13964
                     /locus_tag="OO249_002238"
     CDS             13569..13964
                     /locus_tag="OO249_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5636526.1"
                     /translation="MKHTELRAAVLDALEKHDSGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            13972..14712
                     /locus_tag="OO249_002239"
     CDS             13972..14712
                     /locus_tag="OO249_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5636527.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDKDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAVVAEITVTDS"
     gene            14728..15150
                     /locus_tag="OO249_002240"
     CDS             14728..15150
                     /locus_tag="OO249_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5636528.1"
                     /translation="MFLKTESFEYNGVSVTLSELSALQRIEHLALLKRQAEQAGSSLN
                     RQVSVEDLVRTGAFLVAMSLWHSHPQKTKMPSMNEAVKQIEQEVLTTWPTEAIAQAEN
                     VVMRLSGMSEFVVNDAPEQADDAGPAEPVSAGKCSTVS"
     gene            15132..15566
                     /locus_tag="OO249_002241"
     CDS             15132..15566
                     /locus_tag="OO249_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5636529.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSIHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            15559..18120
                     /locus_tag="OO249_002242"
     CDS             15559..18120
                     /locus_tag="OO249_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5636530.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKVGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SQLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQCRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            18117..>18424
                     /locus_tag="OO249_002243"
     CDS             18117..>18424
                     /locus_tag="OO249_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5636531.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     "
BASE COUNT         4482 a         4445 c         5493 g         4004 t
ORIGIN      
        1 atgttactcc ggtgcttgat ggtgtggatg gacaggcgtt tgacctggtg aaatgggcca
       61 atactgctaa tggactcaac aggtttgtca ttacggatat tacaggtagt gcagaaacct
      121 accctgtact tattgatacg gtggaagtta aagcaaacaa agctggagca gccgcataat
      181 tgctaaaaaa agccgccagc ggcaggaatg gaagctggcg gaggtaatcc caatggagaa
      241 tgtaaagaaa agatgctttc gtacattggt tttttaaatg aaaacaattc tcattgtcaa
      301 ccataacggt aagaaattat gacatttatt catcaggtga tgctgtactt ctgtacggcg
      361 gtctgtgtgc tgtatcttct ttcgggtggg tacagggctg tgcgcgattt ctggtgcagg
      421 cagattgata aaagggccgc tgagaaaatc agcgccagtc agtcagccgg aagcaaaccc
      481 gaagaacccg ttactcctta acaacccctt tcagcgagaa aatcccatgt cagaaatcac
      541 atccctggtc actgcagagg cagtgaagga agtcctgcgc tctgaagaag tccggagcgc
      601 actgaaacag aaacttcgcc agaatcttga gtcgcgtctt gatgcagaag tggatgccat
      661 tctggatgag ctgctgggcg tagcagcggt tcctgagccg gaaggtatcg cgggtgacgg
      721 gagtgcttca gatggcggtg aacctacacc tgacagcgac atgatgatgt aagcatgcgc
      781 aagggaccat cggtgtgtgc cggtggtctt tatattgttg tgagcttccg gattgcggga
      841 gacggggtat gtaccagatg gaaaaaatca caacaggtgt gtcatacacc acgtcagcgg
      901 tgggaacggg ctactggttc ctgcagttgc tggacagggt ttccccgtct cagtgggcgg
      961 caataggcgt gctggggagt ctgctgtttg ggctgctgac atatctgact aacctgtatt
     1021 tcaaaatcag agaggaccgt cgtaaggctg cacggggaga gtaattcaat gactcaaaac
     1081 tataaactga ttgtgaaagg gatccgcaat tttgagaata aagttacggt aactttagcg
     1141 ttacgggaca aaaaacgctt tgacggtgaa atttttgacc tggacatctc gctggaccgt
     1201 gttgaaggtg ccgcgctgga gttttatgag gcagcagcca gaaggagcat cagacaggtc
     1261 ttcctggatg ttgctgccgg gttatgtgaa ggggatgagc agtcgccgga aaagcgcccc
     1321 gtaattttag aggcgcagaa tgtatggata acctacaaag gaaagctacc gggaagaatt
     1381 actggttctc tgaagactcc tccggaatca caaccttaag tcactgaccg gaacagataa
     1441 acctgtccgt gggcagaaac cgataaatcc tgataaatat ccatgaacgc aaaaatcaga
     1501 tacggcctgt cggctgccgt tctggcgctg attgccgctg gtgcgcctgc gcctgacatt
     1561 ctcgaccagt ttctggatga aaaggaaggt aaccacacca cggcataccg tgatggcgcg
     1621 ggtatctgga ccatctgccg cggtgccatc ctggtggatg gcaaacctgt cgttccgggc
     1681 atgaagttgt cgaaggaaaa atgcgaccgg gttaacgcca ttgagcgtga taaggcgctg
     1741 gcatgggtgg agaaaaacat cagagtgcca ttgagtgaac cccagaaagc ggggatcgcg
     1801 tcattctgtc cgtacaacat tggccccggt aagtgttttc cgtcgacgtt ttataaacga
     1861 attaatgcag gtgatcgcag gggagcgtgt gaggcgattc gctggtggat taaggacggt
     1921 ggcagagact gccgtattcg ctcaaacaac tgttacggtc aggtatcccg tcgcgaccag
     1981 gagagtgcgc tggcgtgctg gggaattgac agataagcag aatattttgc tgaaaaatgc
     2041 ggtttgctca cacggacgga taacacgaaa tcctgcgaac tgacaaaaac taagagaata
     2101 aaagtaaaaa ccccgtttgt tggctgcaag cggggttttg tgtttcctga ctccggaaaa
     2161 gtcaaaggag aaagtgtgtt tgattttagc aaactgattc gggagattcg agtgatggct
     2221 gaaaaattat ccacctggaa gttcattctt atctggctgg tgtttgtgat tatggcctcc
     2281 ggttatttca tcggtcagat acgctggtgg tgaaatgaac cgcgtactgt gcgtggtcat
     2341 cattgccctg ctggtggcct gtggtgcgct tagtctgggg ctgaatcatt accgtgataa
     2401 cgccataacc tacaaagagc agcgcgataa agccacatcc acaatcgctg atatgcagaa
     2461 gcgtcaacgt gacgtagcag aactcgatgc cagatataca aaggagcttg ctgatgctaa
     2521 cgcgactatc gaaagcctcc gtgctgatgt ttctgctggg cgtaagcgcc tgcaagtcgc
     2581 cgctacctgt gcaaagtcaa cgaccggagc cagcggcatg ggcaatggag aaagcccaag
     2641 acttacagca gatgctgaac tcaattatta ccgtctccga agtggaatcg acaagataac
     2701 cgcgcaggtc aactacctgc aggagtacat caggacgcag tgcttaaaat aattttaatt
     2761 tcactgaaat ttaatacgtg actttcagga aaatgcctcg cagatgcggg gcatttttgt
     2821 acaggtattt caccgcgcac cgcagtgcac tcaaccacgt cgaaccaaac cctttggaat
     2881 gagcctttga gtagtcagtt agtgctggtg agccttgacg ggctgatctc ctatgcagca
     2941 aaggttcatc tcaaagtaag gcgaacgcta tgacaaacca agattctata gacctatctg
     3001 atcttcgtgg aatggtcagt tttccagaca aaaggtaatc accatagtcg tatggctatg
     3061 aatcttgttg ctgcagataa gcattttgtg attgaagtgg tcaaatctct caaatgcagt
     3121 aaaatgcagt gcgctataat tcaataacgg agggagtaag gagaagtcat gaaagatcaa
     3181 gatgttaggt tcgcggtgca tcataagctt ttgaaagaat cgcatttaga tccagactgc
     3241 cttgtggtcg atgaattttc catatccctt ggcgccagta gagcagacat tgctgtaata
     3301 aatggtgtta tacacgggta cgagctcaaa agtgaatatg actctttgga gcgtttgcct
     3361 cttcaaatca agcattattc ttctgtaatg gacaaggtta ctcttgtcgt agctgagaaa
     3421 catcttgagg gagcattaaa gttaatccca ggttggtggg gcgttaaaac ggtttctgtt
     3481 gggccaaaag gcgccattct tataaagcac atgcgtggag aaaagcttaa tcgaaaccat
     3541 gacacattga tgctcgctca attgctttgg aaagatgaat gtatcgacgt acttgaacga
     3601 tggggctatt ccaaaggaat caaaagcaag ccccgatttg agttatggaa tattattgcg
     3661 gaaaatattc caatagcgaa tctcaggctt gaagtcagaa cagccttaaa gaaacgcgta
     3721 ggctggaaag ttaaggcttg gcaggctgag tctgcgccaa ccaataaagc tgtctcacga
     3781 ctaacgtaat atggtgcgta tgtgcaactt tacgccattc tttagagccg ccagatttgt
     3841 tagcgcctaa tgatctttgg taaatgtaat catcccccca actaaatttg gaaccaaaga
     3901 cttgatactc tggcgaacta acaagggtag tacataagtt tttagtttgg ccccatccat
     3961 ttcctttaac tgcggtacct tttacaaaga tccatgaggt atcgttcgaa tatctcactg
     4021 agacatactg agacatgaag cgtgggtcta cgctcgtaat ggtagagcta gcggtgggat
     4081 aatcactaaa acttggcgtt cttccattgc taaaattctg tactacgtac atccaaagat
     4141 cgtattcatg gcgcggaata tgatgaactt gatgttgcgg tatccctgcc tgtgatgctg
     4201 ggtatgcggt tgaagataaa atcaagtttc tccacggagc ttggcctgat aatgtgttga
     4261 ccatgcttaa tgcttgttat tttaaactat cagttgcgtt ttgaatatct ccaaaatcaa
     4321 tgattacatc aattaaacta ggatgaatat ttaagtggtt aaccagactt gtgaattggt
     4381 gccatgtctg gggggtgata gatatagcga gcccattgat taagttgcgt tgaacagcat
     4441 gtatatagtt tgttgaatat gcgggagaaa caacagggat aatttcctta ccatttactc
     4501 tagcatcttg gatacacata tctagaggat gatggcggct cgaaccatgt ttatctaaat
     4561 atttaacatc cagcaaaaca ggacgattgg ctttccagga tgccgcaagg ttaataccga
     4621 agtcagataa gtaagaactc aaactcttct tgtagcattc gttttcataa tcccagtcta
     4681 tgtctggaat ggtaatgatc ggagtaaaac cagagagcgt agtttgatct aaaagcatca
     4741 gagattcata ttcagcaggc ttccatttca gctgtggata atattgatgt tgactcatta
     4801 aaactcctta aaatctttac ttatcctaag tatagtgtta tggcgtaacg ccaattgttt
     4861 ttatacactt gaaaacattg ggaaaagttg agctatatct aaatttacaa aattggatgt
     4921 tatagaaatc gaggtttcaa ctaggcatgg aactgcaaaa tttttaatgc ctgcacgcga
     4981 aagtcgttgg cgggtccttt ccggtgatcc agaccgttac ggggcggcga cctcgcgggt
     5041 tttcgctatt tatgaaaatt ttccggttta aggcgtttcc gttcttcttc gtcgtaactt
     5101 aatgttttta tttaaaatac cccctgaaaa gaaaggaaac gacaggtgct gaaaacgaac
     5161 ttttgggcct ttgtcgtttc ctttctctgt ttttggccgt ggaatgaaca atggaagtca
     5221 acaaaaagca gctggctgac attttcggtg cgagtatccg taccattcag aactggcagg
     5281 aacagggaat gcccgttctg cgaggcggtg gcaagggtaa tgaggtgctt tatgactctg
     5341 ccgccgttat aaaatggtat gccgaaaggg atgctgaaat tgagaacgaa aagctgcgcc
     5401 gggaggttga agaactgcgc caggccagcg aggcagatct ccagccaggg actattgagt
     5461 acgaacgcca tcgacttacg cgtgcgcagg ccgacgcaca ggaactgaag aatgccagag
     5521 actcagctga agtggtggaa accgcattct gtactttcgt gctgtcgcgg atcgcaggtg
     5581 aaattgccag tattctcgac gggatccccc tgtcggtgca gcggcgtttt ccggaactgg
     5641 aaaaccgaca tgttgatttc ctgaaacggg atatcatcaa agccatgaac aaagcagccg
     5701 cgctggatga actgataccg gggttgctga gtgaatatat cgaacagtca ggttaacagg
     5761 ctgcggcatt ttgtccgcgc cgggcttcgc tcactgttca ggccggagcc acagaccgcc
     5821 gttgaatggg cggatgctaa ttactatctc ccgaaagaat ccgcatacca ggaagggcgc
     5881 tgggaaacac tgccctttca gcgggccatc atgaatgcga tgggcagcga ctacatccgt
     5941 gaggtgaatg tggtgaagtc tgcccgtgtc ggttattcca aaatgctgct gggtgtttat
     6001 gcctacttta tagagcataa gcagcgcaac acccttatct ggttgccgac ggatggtgat
     6061 gccgagaact ttatgaaaac ccacgttgag ccgactattc gtgatattcc gtcgctgctg
     6121 gcgctggccc cgtggtatgg caaaaagcac cgggataaca cgctcaccat gaagcgtttc
     6181 actaatgggc gtggcttctg gtgcctgggc ggtaaagcgg caaaaaacta ccgtgaaaag
     6241 tcggtggatg tggcgggtta tgatgaactt gctgcctttg atgaggatat tgaacaggaa
     6301 ggctctccga cgttccttgg cgacaaacgt attgaaggct cggtctggcc aaagtccatc
     6361 cgtggctcca cgcccaaagt gagaggcacc tgccagattg agcgtgcagc cagtgaatcc
     6421 ccgcatttta tgcgttttca tgttgcctgc ccgcactgcg gggaggagca gtatcttaaa
     6481 tttggcgaca aagagacgcc gtttggcctc aaatggacgc cggatgaccc ctccagcgtg
     6541 ttttatctct gcgagcataa tgcctgcgtc atccgccagc aggagctgga ctttactgat
     6601 gcccgttata tctgcgaaaa gaccgggatc tggacccgtg atggcattct ctggttttcg
     6661 tcatccggtg aagagattga gccacctgac agtgtgacct ttcacatctg gacagcgtac
     6721 agcccgttca ccacctgggt gcagattgtc aaagactgga tgaaaacgaa aggggatacg
     6781 ggaaaacgta aaaccttcgt aaacaccacg ctcggtgaga cgtgggaggc gaaaattggc
     6841 gaacgtccgg atgctgaagt gatggcagag cggaaagagc attattcagc gcccgttcct
     6901 gaccgtgtgg cttacctgac cgccggtatc gactcccagc tggaccgcta cgaaatgcgc
     6961 gtatggggat gggggccggg tgaggaaagc tggctgattg accggcagat tattatgggc
     7021 cgccacgacg atgaacagac gctgctgcgt gtggatgagg ccatcaataa aacctatacc
     7081 cgccggaatg gtgcagaaat gtcgatatcc cgtatctgct gggatactgg cgggattgac
     7141 ccgaccattg tgtatgaacg ctcgaaaaaa catgggctgt tccgggtgat ccccattaaa
     7201 ggggcatccg tctacggaaa gccggtggcc agcatgccac gtaagcgaaa caaaaacggg
     7261 gtttacctta ccgaaatcgg tacggatacc gcgaaagagc agatttataa ccgcttcaca
     7321 ctgacgccgg aaggggatga accgcttccc ggtgccgttc acttcccgaa taacccggat
     7381 atttttgatc tgaccgaagc gcagcagctg actgctgaag agcaggtcga aaaatgggtg
     7441 gatggcagga aaaaaatact gtgggacagc aaaaagcgac gcaatgaggc gctcgactgc
     7501 ttcgtttatg cgctggcggc gctgcgcatc agtatttccc gctggcagct ggatctcagt
     7561 gcactgctgg cgagcctgca ggaagaggat ggtgcagcaa ccaacaagaa aacactggca
     7621 gattacgccc gtgccttatc cggagaggat gaatgacgcg acaggaagaa cttgccgctg
     7681 cccgtgcggc actgcatgac ctgatgacag gaaaacgggt ggcaacggta cagaaagacg
     7741 gacggagagt ggagtttacg gccacttccg tgtctgacct gaaaaaatac attgcggagc
     7801 tggaagtgca gaccggcatg acacagcgac gcaggggacc tgcaggattt tatgtatgaa
     7861 aacgtccacc attcccaccc ttctggggcc ggacggcatg acatcgctgc gtgaatatgc
     7921 cggttatcac ggcggtggca gcggatttgg agggcagttg cgggcgtgga acccaccgag
     7981 tgaaagtgtg gatgcagccc tgctgcccaa ctttacccgt ggcaatgccc gcgcagacga
     8041 tctggtacgc aataacggct atgccgccaa cgccatccag ttgcatcagg atcatatcgt
     8101 cgggtctttt ttccggctca gtcatcgccc aagctggcgc tatctgggca tcggggagga
     8161 agaagcccgt gccttttccc gcgaggttga agcggcatgg aaagagtttg ccgaggatga
     8221 ctgctgctgc attgacgttg agcgaaaacg cacgtttacc atgatgattc gggaaggtgt
     8281 ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc acctgggata ccagttcgtc
     8341 gcggcttttc cggacacagt tccggatggt cagcccgaag cgcatcagca acccgaacaa
     8401 taccggcgac agccggaact gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct
     8461 gggatattac gtcagcgagg acgggtatcc tggctggatg ccgcagaaat ggacatggat
     8521 accccgtgag ttacccggcg ggcgcgcctc gttcattcac gtttttgaac ccgtggagga
     8581 cgggcagact cgcggtgcaa atgtgtttta cagcgtgatg gagcagatga agatgctcga
     8641 cacgctgcag aacacgcagc tgcagagcgc cattgtgaag gcgatgtatg ccgccaccat
     8701 tgagagtgag ctggatacgc agtcagcgat ggattttatt ctgggcgcga acagtcagga
     8761 gcagcgggaa aggctgaccg gctggattgg tgaaattgcc gcgtattacg ccgcagcgcc
     8821 ggtccggctg ggaggcgcaa aagtaccgca cctgatgccg ggtgactcac tgaacctgca
     8881 gacggctcag gatacggata acggctactc cgtgtttgag cagtcactgc tgcggtatat
     8941 cgctgccggg ctgggtgtct cgtatgagca gctttcccgg aattacgccc agatgagtta
     9001 ctccacggca cgggccagtg cgaacgagtc gtgggcgtac tttatggggc ggcgaaaatt
     9061 cgtcgcatcc cgtcaggcga gccagatgtt tctgtgctgg ctggaagagg ccatcgttcg
     9121 ccgcgtggtg acgttacctt caaaagcgcg cttcagtttt caggaagccc gcagtgcctg
     9181 ggggaactgc gactggatag gctccggtcg tatggccatc gatggtctga aagaagttca
     9241 ggaagcggtg atgctgatag aagccggact gagcacctac gagaaagagt gcgcgaaacg
     9301 cggtgacgac tatcaggaaa tttttgccca gcaggtccgt gaaacgatgg agcgccgtgc
     9361 agccggtctt aaaccgcccg cctgggcggc tgcagcattt gaatccgggc tgcgacaatc
     9421 aacagaggag gagaagagtg acagcagagc tgcgtaatct cccgcatatt gccagcatgg
     9481 cctttaatga gccgctgatg cttgaacccg cctatgcgcg ggttttcttt tgtgcgcttg
     9541 caggccagct tgggatcagc cgcctgacgg atgcggtgtc cggcgacagc ctgactgccc
     9601 aggaggcact cgcgacgctg gcattatccg gtgatgatga cggaccacga caggcccgca
     9661 gttatcaggt catgaacggc atcgccgtgc tgccggtgtc cggcacgctg gtcagccgga
     9721 cgcgggcgct gcagccgtac tcggggatga ccggttacaa cggcattatc gcccgtctgc
     9781 aacaggctgc cagcgatccg atggtggacg gcattctgct cgatatggac acgcccggcg
     9841 ggatggtggc gggggcattt gactgcgctg acatcatcgc ccgtgtgcgt gacataaaac
     9901 cggtatgggc gcttgccaac gacatgaact gcagtgcagg tcagttgctt gccagtgccg
     9961 cctcccggcg tctggtcacg cagaccgccc ggacaggctc catcggcgtc atgatggctc
    10021 acagtaatta cggtgctgcg ctggagaaac agggtgtgga aatcacgctg atttacagcg
    10081 gcagccataa ggtggatggc aacccctaca gccatcttcc ggatgacgtc cgggagacac
    10141 tgcagtcccg gatggacgca acccgccaga tgtttgcgca gaaggtgtcg gcatataccg
    10201 gcctgtccgt gcaggctgtg ctggataccg aggctgcagt gtacagcggt caggaggcca
    10261 ttgatgccgg actggctgat gaacttgtta acagcaccga tgcgatcacc gtcatgcgtg
    10321 atgcactgga tgcacgtaaa tcccgtctct caggagggcg aatgaccaaa gagactcaat
    10381 caacaactgt ttcagccact gcttcgcagg ctgacgttac tgacgtggtg ccagcgacgg
    10441 agggcgagaa cgccagcgcg gcgcagccgg acgtgaacgc gcagatcacc gcagcggttg
    10501 cggcagaaaa cagccgcatt atggggatcc tcaactgtga ggaggctcac ggacgcgaag
    10561 aacaggcacg cgtgctggca gaaacccccg gtatgaccgt gaaaacggcc cgccgcattc
    10621 tggccgcagc accacagagt gcacaggcgc gcagtgacac tgcgctggat cgtctgatgc
    10681 agggggcacc ggcaccgctg gctgcaggta acccggcatc tgatgccgtt aacgatttgc
    10741 tgaacacacc agtgtaaggg atgtttatga cgagcaaaga aacctttacc cattaccagc
    10801 cgcagggcaa cagtgacccg gctcataccg caaccgcgcc cggcggattg agtgcgaaag
    10861 cgcctgcaat gaccccgctg atgctggaca cctccagccg taagctggtt gcgtgggatg
    10921 gcaccaccga cagtgctgcc gttggcattc ttgcggttgc tgctgaccag accagcacca
    10981 cgctgacgtt ctacaagtcc ggcacgttcc gttatgagga tgtgctctgg ccggaggctg
    11041 ccagcgacga gacgaaaaaa cggaccgcgt ttgccggaac ggcaatcagc atcgtttaac
    11101 tttacccttc atcactaaag gccgcctgtg cggctttttt tacgggattt ttttatgtcg
    11161 atgtacacaa ccgcccaact gctggcggca aatgagcaga aatttaagtt tgatccgctg
    11221 tttctgcgtc tctttttccg tgagagctat cccttcacca cggagaaagt ctatctctca
    11281 caaattccgg gactggtaaa catggcgctg tacgtttcgc cgattgtttc cggtgaggtt
    11341 atccgttccc gtggcggctc cacctctgaa tttacgccgg gatatgtcaa gccgaagcat
    11401 gaagtgaatc cgcagatgac cctgcgtcgc ctgccggatg aagatccgca gaatctggcg
    11461 gacccggctt accgccgccg tcgcatcatc atgcagaaca tgcgtgacga agagctggcc
    11521 attgctcagg tcgaagagat gcaggccgtt tctgccgtgc tcaagggcaa atacaccatg
    11581 accggtgaag ccttcgatcc ggttgaggtg gatatgggcc gcagtgcggc gaacaacatc
    11641 acgcagtccg gcagcacgga gtggagcaag cgtgacaagt ccacgtatga cccgaccgac
    11701 gatatcgaag cctacgcgct gaacgccagc ggcgtggtga atatcatcgt gtttgatccg
    11761 aaaggctggg cgctgttccg ttccttcaaa gccgtcaggg agaagctgga tacccgtcgc
    11821 ggctctcatt ccgagctgga gacagcggtg aaagacctgg gtaaagcggt gtcctataag
    11881 gggatgtatg gcgatgtggc catcgtcgtg tattccggac agtacgtgga aaacggcgtc
    11941 aaaaagaact tcctgccgga caacacgatg gtactgggga acactcaggc acgcggtctg
    12001 cgcacctatg gctgcattca ggatgcggac gcacagcgcg aaggcattaa cgcctctgcc
    12061 cgttacccga aaaactgggt gaccaccggc gatccggcgc gtgagttcac catgattcag
    12121 tcagcaccgc tgatgctgct ggctgaccct gatgagttcg tgtccgtaca actggcgtaa
    12181 tcgtggccct tcggggccat tttctctctg tggaggagtc catgacgaaa gatgaactga
    12241 ttgtccgtct ccggtcgctg ggtgagcaac tgaaccgtga tgtcagcctg acggggacga
    12301 aagaagaact ggcgctccgt gtggcagagc tggaagagga gcttgatgac acggatgacg
    12361 ctgccggtca ggatacccct ctcagaccgg aaaatgcgct gaccggacat gaaaatgagg
    12421 tggtatcagc acagacggat accgtgactg atgcggctgc tctggtcacg gttgtggcac
    12481 tggtgacgct gcataccgat gcacttcacg ccacgcggga tgaaccagtg gcatttgtgc
    12541 tgccggaaac ggcgttccgt gtctctgccg gtgtggcagc tgaaatgaca gagcgcggcc
    12601 tggccagaat gcaataacgg gaggcgctgt ggctgatttc gataacctgt tcgatgctgc
    12661 cattgcccgc gccgatgaaa cgatacgcgg gtacatggga acgtcagcca ccatgacatc
    12721 cggtgagcag tccggcgcag taatacgtgg tgtttttgat gaccctgaaa atatcagcta
    12781 tgccggacag ggcgtacgcg ttgaaggctc cagcccgtcc ctgtttgtcc ggactgatga
    12841 ggtgcggcag ctgcggcgcg gcgacacgct gaccatcggt gaggaaaact tctggataga
    12901 ccggatttcg ccggatgatg gcggaagctg tcatctctgg cttgggcggg gcgtaccgcc
    12961 tgccgttaac cgtcgccgct gaaaggggga tgtatggcca taaaaggtct tgagcaggcc
    13021 gttgaaaacc tcagccgtat cagtaaaacg gcggtgcccg gtgccgccgc aatggccatt
    13081 aaccgcgttg cttcatccgc gatatcgcag tcggcgtcac aggttgctcg tgagacaaag
    13141 gtacgccgga aactggtaaa ggaaagggcc aggctgaaaa gggccacggt caaaaatccg
    13201 caggccagaa tcaaagttaa ccggggggat ttgcccgtaa tcaagctggg taatgcgcgg
    13261 gttgtccttt cgcgccgcag gcgtcgtaaa aaggggcagc gttcatccct gaaaggtggc
    13321 ggcagcgtgc ttgtggtggg aaaccgtcgt attcccggcg cgtttattca gcaactgaaa
    13381 aatggccgct ggcatgtcat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg
    13441 gtgaaaatcc cgatggcggt gccgctgacc acggcgttta aacagaatat tgaacggata
    13501 cggcgtgaac gtcttccgaa agagctgggc tatgcgctgc agcatcaact gagaatggta
    13561 ataaagcgat gaaacatact gaactccgtg cagccgtact ggatgcactg gagaagcatg
    13621 acagcggggc gacgcttttt gatggtcgcc ccgctgtttt tgatgaggaa gattttccgg
    13681 caattgccgt ttatctcacc ggcgctgaat acacgggcga agagctggac agcgatacct
    13741 ggcaggcgga gctgcatatt gaagttttcc tgcctgctca ggtgccggat tcagagctgg
    13801 atgcgtggat ggagtcccgg atttatccgg tgatgagcga tatcccggca ctgtcagatt
    13861 tgatcaccag tatggtggcc agtggctatg actaccggcg cgacgatgat gcgggcctgt
    13921 ggagttcagc cgatctgact tatgtcatta cctatgaaat gtgaggacgc tatgcctgta
    13981 ccaaatccta caatgccggt gaaaggtgcc gggaccaccc tgtgggttta taaggggagc
    14041 ggtgaccctt atgcgaaccc gctttcagac gttgactggt cgcgtctggc aaaagttaaa
    14101 gacctgacgc ccggcgaact gaccgctgag tcctatgacg acagctatct cgatgataaa
    14161 gatgcagact ggactgcgac cgggcagggg cagaaatctg ccggagatac cagcttcacg
    14221 ctggcgtgga tgcccggaga gcaggggcag caggcgctgc tggcgtggtt taatgaaggt
    14281 gatacccgtg cctataaaat ccgcttcccg aacggcacgg tcgatgtgtt ccgtggctgg
    14341 gtcagcagta tcggtaaggc ggtgacggcg aaggaagtga ttacccgcac ggtgaaggtc
    14401 accaatgtgg gacgtccgtc gatggcagaa gatcgcagca cggtaacagc ggcaaccggc
    14461 atgaccgtga cgcctgccag cacttcggtg gtgaaagggc agagcaccac gctgaccgtg
    14521 gcattccagc cggaaggcgc aaccgacaag agcttccgtg cggtgtctgc ggataaaaca
    14581 aaagccaccg tgtcggtcag tggtatgacc atcaccgtga aaggtgttgc tgcaggcaag
    14641 gtcaacattc cggttgtatc cggtaatggt gaacttgctg tggttgcaga aatcaccgtc
    14701 accgacagtt aatccggaga gtcagcgatg ttcctgaaaa ccgaatcatt tgaatataac
    14761 ggtgtgagcg tcacgctttc tgaactgtca gccctgcagc gaattgagca tctcgccctg
    14821 ctgaaacgac aggcagaaca ggcgggatcc agtctcaatc gacaggtgag cgtggaagat
    14881 ctcgtcagaa ccggtgcttt tctggtggcg atgtccctgt ggcatagcca tccgcagaag
    14941 acaaagatgc cgtccatgaa tgaagccgtt aaacaaattg agcaggaagt gcttaccacc
    15001 tggcccacag aggcaattgc tcaggctgaa aatgtggtaa tgcgtctgtc cggtatgtct
    15061 gagtttgttg tgaatgatgc acctgaacag gcagatgacg ccgggccagc agagcctgtt
    15121 tctgcgggaa agtgttcgac ggtgagctga gttttgccct gaaactggcg cgtgagatgg
    15181 ggcgacccga ctggcgcgcc atgcttgccg ggatgtcatc cacggagtat gccgactggc
    15241 accgctttta cagtatccat tattttcatg atgttctgct ggatatgcac ttttccgggc
    15301 tgacgtacac cgtactcagc ctgtttttca gcgatccgga tatgcatccg ctggatttca
    15361 gtctgctgaa ccggcgcgag gctgacgaag agcctgaaga tgatgtgctg atgcagaaag
    15421 cggcagggct tgccggaggc gtccgctttg gcccggacgg gaatgaagtt atccccgctt
    15481 ccccggatgt ggcggacatg acggaggatg acgtaatgct gatgacagta tcagaaggga
    15541 tcgcaggagg agtccggtat ggctgaaccg gtaggcgatc tggtcgttga tttgagtctg
    15601 gatgcggcca gatttgacga gcagatggcc agagtcaggc gtcatttttc cggtacggaa
    15661 agtgatgcga aaaaaacagc ggcagtcgtt gaacagtcga tgaaccggca ggcgctggct
    15721 gcacagaaag tgggaatttc cgtcgggcag tataaagccg ccatgcgtat gctgcctgcg
    15781 cagttcactg acgtggccac gcagcttgca ggcgggcaaa gtccgtggct gatcctgctg
    15841 caacaggggg gtcaggtgaa ggactccttc ggcgggatga tccccatgtt ccgggggctt
    15901 gccggtgcga tcaccctgcc gatggtgggg gccacctcgc tggcggtggc gaccggagcg
    15961 ctggcgtatg cctggtatca gggcaactca accctgtccg atttcaacaa aacgctggtc
    16021 ctttccggca atcaggcggg actgacggca gatcgtatgc tggtcctgtc cagagccggg
    16081 caggcggcag ggctgacgtt taaccagacc agcgagtcac tcagcgcact ggttaaggcg
    16141 ggggtaagcg gtgaggctca gattgcgtcc atcagccaga gtgtggcgcg tttctcctct
    16201 gcatccggcg tggaggtgga caaggtcgct gaagccttcg ggaagctgac cacagacccg
    16261 acgtcggggc tgacggcgat ggcacgccag ttccataacg tgacggcgga gcagattgcg
    16321 tatgttgctc agttgcagcg ttccggcgat gaagccgggg cattgcaggc ggcgaacgag
    16381 gccgcaacga aagggtttga tgaccagacc cgacgcctga aagagaacat gggcacgctg
    16441 gaaacctggg cagacaggac agcacgggca ttcaaatcca tgtgggatgc ggtgctggat
    16501 attggtcgtc ctgataccgc tcaggagatg ctgattaagg cagaggctgc gtttaagaaa
    16561 gcagacgaca tctggaatct gcgcaaggat gattattttg ttaacgatga agcgcgggcg
    16621 cgttactggg atgatcgtga aaaggcccgt cttgcgcttg aagccgcccg aaagaaggct
    16681 gagcagcaga ctcaacagga caaaaatgcg cagcagcaga gcgatactga agcgtcacag
    16741 ctgaaatata ccgaagaggc gcagaaggct tacgaacggc tgcagacgcc gctggagaaa
    16801 tataccgccc gtcaggaaga actgaacaag gcactgaaag acgggaaaat cctgcaggcg
    16861 gattacaaca cgctgatggc ggcggcgaaa aaggattatg aagcgacgct gaaaaagccg
    16921 aaacagtccg gcgtgaaggt gtctgcgggc gatcgtcagg aagacagtgc tcatgctgcc
    16981 ctgctgacgc ttcaggcaga actccggacg ctggagaagc atgccggagc aaatgagaaa
    17041 atcagccagc agcgccggga tttgtggaag gcggagagtc agttcgcggt actggaggag
    17101 gcggcgcaat gtcgccagct gtctgcacag gagaaatccc tgctggcgca taaagatgag
    17161 acgctggagt acaaacgcca gctggctgca cttggcgaca aggttacgta tcaggagcgc
    17221 ctgaacgcgc tggcgcagca ggcggataaa ttcgcacagc agcaacgggc aaaacgggcc
    17281 gccattgatg ctaaaagccg ggggctgact gaccggcagg cagaacggga agccacggaa
    17341 cagcgcctga aggaacagta tggcgataat ccgctggcgc tgaataacgt catgtcagag
    17401 cagaaaaaga cctgggcggc tgaagaccag cttcgcggga actggatggc aggcctgaag
    17461 tccggctgga gtgagtggga agagagcgcc acggacagta tgtcgcaggt aaaaagtgca
    17521 gccacgcaga cctttgatgg tattgcacag aatatggcgg cgatgctgac cggcagtgag
    17581 cagaactggc gcagcttcac ccgttccgtg ctgtccatga tgacagaaat tctgcttaag
    17641 caggcaatgg tggggattgt cgggagtatc ggcagcgcca ttggcggggc tgttggtggc
    17701 ggcgcatccg cgtcaggcgg tacagccatt caggccgctg cggcgaaatt ccattttgca
    17761 accggaggat ttacgggaac cggcggcaaa tatgagccag cggggattgt tcaccgtggt
    17821 gagtttgtct tcacgaagga ggcaaccagc cggattggcg tggggaatct ttaccggctg
    17881 atgcgcggct atgccaccgg cggttatgtc ggtacaccgg gcagcatggc agacagccgg
    17941 tcgcaggcgt ccgggacgtt tgagcagaat aaccatgtgg tgattaacaa cgacggcacg
    18001 aacgggcaga taggtccggc tgctctgaag gcggtgtatg acatggcccg caagggtgcc
    18061 cgtgatgaaa ttcagacaca gatgcgtgat ggtggcctgt tctccggagg tggacgatga
    18121 agaccttccg ctggaaagtg aaacccggta tggatgtggc ttcggtccct tctgtaagaa
    18181 aggtgcgctt tggtgatggc tattctcagc gagcgcctgc cgggctgaat gccaacctga
    18241 aaacgtacag cgtgacgctt tctgtccccc gtgaggaggc cacggtactg gagtcgtttc
    18301 tggaagagca cgggggctgg aaatcctttc tgtggacgcc gccttatgag tggcggcaga
    18361 taaaggtgac ctgcgcaaaa tggtcgtcgc gggtcagtat gttgcgtgtt gagttcagcg
    18421 caga
//
LOCUS       DAJTOR010000075        18277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTOR010000075 DAJTOR010000000
VERSION     DAJTOR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.075"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..1338)
                     /gene="glnS"
                     /locus_tag="OO249_002244"
     CDS             complement(<1..1338)
                     /gene="glnS"
                     /locus_tag="OO249_002244"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5636532.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTRQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSSEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKD"
     gene            complement(1541..3487)
                     /gene="nagE"
                     /locus_tag="OO249_002245"
     CDS             complement(1541..3487)
                     /gene="nagE"
                     /locus_tag="OO249_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5636533.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            3820..4620
                     /gene="nagB"
                     /locus_tag="OO249_002246"
     CDS             3820..4620
                     /gene="nagB"
                     /locus_tag="OO249_002246"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5636534.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            4680..5828
                     /gene="nagA"
                     /locus_tag="OO249_002247"
     CDS             4680..5828
                     /gene="nagA"
                     /locus_tag="OO249_002247"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5636535.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            5837..7057
                     /gene="nagC"
                     /locus_tag="OO249_002248"
                     /gene_synonym="nagR"
     CDS             5837..7057
                     /gene="nagC"
                     /locus_tag="OO249_002248"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5636536.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYIGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7105..7857
                     /gene="nagD"
                     /locus_tag="OO249_002249"
     CDS             7105..7857
                     /gene="nagD"
                     /locus_tag="OO249_002249"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR5636537.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETSSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8254..9918
                     /gene="asnB"
                     /locus_tag="OO249_002250"
     CDS             8254..9918
                     /gene="asnB"
                     /locus_tag="OO249_002250"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5636538.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10299..10375
                     /locus_tag="OO249_002251"
     tRNA            10299..10375
                     /locus_tag="OO249_002251"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10333..10335,aa:Met,seq:cat)
     gene            10384..10468
                     /locus_tag="OO249_002252"
     tRNA            10384..10468
                     /locus_tag="OO249_002252"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10418..10420,aa:Leu,seq:tag)
     gene            10492..10566
                     /locus_tag="OO249_002253"
     tRNA            10492..10566
                     /locus_tag="OO249_002253"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10524..10526,aa:Gln,seq:ttg)
     gene            10601..10675
                     /locus_tag="OO249_002254"
     tRNA            10601..10675
                     /locus_tag="OO249_002254"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10633..10635,aa:Gln,seq:ttg)
     gene            10691..10767
                     /locus_tag="OO249_002255"
     tRNA            10691..10767
                     /locus_tag="OO249_002255"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10725..10727,aa:Met,seq:cat)
     gene            10816..10890
                     /locus_tag="OO249_002256"
     tRNA            10816..10890
                     /locus_tag="OO249_002256"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10848..10850,aa:Gln,seq:ctg)
     gene            10928..11002
                     /locus_tag="OO249_002257"
     tRNA            10928..11002
                     /locus_tag="OO249_002257"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10960..10962,aa:Gln,seq:ctg)
     gene            complement(11156..12331)
                     /gene="ubiF"
                     /locus_tag="OO249_002258"
     CDS             complement(11156..12331)
                     /gene="ubiF"
                     /locus_tag="OO249_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5636539.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12477..13901
                     /gene="miaB"
                     /locus_tag="OO249_002259"
     CDS             12477..13901
                     /gene="miaB"
                     /locus_tag="OO249_002259"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5636540.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPSETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14054..15094
                     /locus_tag="OO249_002260"
     CDS             14054..15094
                     /locus_tag="OO249_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5636541.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15091..15558
                     /gene="ybeY"
                     /locus_tag="OO249_002261"
     CDS             15091..15558
                     /gene="ybeY"
                     /locus_tag="OO249_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5636542.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15648..16526
                     /gene="corC"
                     /locus_tag="OO249_002262"
     CDS             15648..16526
                     /gene="corC"
                     /locus_tag="OO249_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5636543.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16551..18089
                     /gene="lnt"
                     /locus_tag="OO249_002263"
     CDS             16551..18089
                     /gene="lnt"
                     /locus_tag="OO249_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5636544.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
BASE COUNT         4436 a         4810 c         4732 g         4299 t
ORIGIN      
        1 atctttgctt aaggtgtcgg cgtcataagt acagaagatg gtggtgatat tgccttcggc
       61 atctttctcc acgcgttctg ccttaatgac gtaagcatta cgcagacgca cttctttacc
      121 cagcaccaga cgtttgtact gcttgttagc ttcttcgcgg aaatcggcgc gatcgatcca
      181 aatctcactg ctaaacggca cctggcggct acccatttcc ggtttgttcg gatggttcgg
      241 catggtaacc atttcgcctt cgccctgata gttttcgata accagtttca ccggatcgat
      301 aaccgccatt gcgcgcgggg cgttttcgtt gagatcttca cggatgcagg attccagcga
      361 cgccatctca atggtgttgt cctgcctggt cacgccgatg cgtttgcaga actcacgaat
      421 agaagcagca gtgtaaccac gacgacgcag accggaaatg gtcggcatac gcgggtcatc
      481 ccagccttca acgtgcttgt cggtcaccag caggttcagc ttacgcttgg acatcacagt
      541 gtattccaga ttcaggcgcg agaattcgta ctggcgcggg tgaacaggaa tggtgatgtt
      601 gtccagcacc cagtcgtaca ggcgacggtt gtcctggaac tcaagcgtac acagagagtg
      661 cgtaatacct tccagggcat cgctgatgca gtgggtgaag tcgtacatcg ggtagatgca
      721 ccacttgttg ccagtctggt ggtgttcagc aaatttaatg cggtacagca ccggatcgcg
      781 catcacgata aacggtgaag ccatgtcgat tttcgcacgc aggcaggctt taccttcttc
      841 aaaaccaccg gcacgcattt tttcgaacag cgccaggttc tcttcaacgc tgcggtcgcg
      901 gtacgggctg tttttacccg gttgcgtcag ggtgccgcgg tattcgcgga tctgttccgg
      961 cgtcagttca tcaacgtacg ccaggccttt attgatcagt tcgatcgcat aggcgtggag
     1021 ctgatcaaaa taatcggagg agtaacggac gttaccagac cagtgaaaac ctaaccactc
     1081 tacgtcgttt ttgatcgact caacatactc gatatcttct tttaccgggt tagtgtcgtc
     1141 gaaacgcagg ttgcactggc ctttatagtc ctgggcgatc ccgaagttca ggcagataga
     1201 tttcgcatgg ccaatatgca gatagccatt cggctccggc gggaaacggg tgtgtactgt
     1261 ggtgtgctta ccactggcca gatcttcatc gatgatctga cggataaagt tagtcgggcg
     1321 ggcttctgcc tcactcatcg tggattcctc aaagcgtaaa caacgtataa cggcgtatga
     1381 tcttataagc ggaacaggct gacaactgtt agttttttaa aattgataat ctaacaagga
     1441 ttatggggat gattgatgaa aaaaaaagcg gcagagaaga tctccaccgc ttgtcattgt
     1501 tggatgcgac gctcaagcgt cgcatcaggc ataaagcaga ttactttttg atttcataca
     1561 gcggtgtttg acccgccaca acatggccct gagctttaat gatcaagcca ctgaaatcgt
     1621 cgatattgct gcaaaccacc gggctaatca tcgagcgggc gttagcgttc aggtaatcca
     1681 gatccatttc cagaatcggt tgccctgcgc ttacctgcgc accctcttcc accagacgtt
     1741 taaagccttt accttccagc gctacggtgt cgatacccat atggacgacg atctccgcgc
     1801 ctttttcggt ttccaggcag aacgcgtggt tggtgttgaa gattttcacg attgtccctg
     1861 cggctggtga tacgacgatt ttatctgtcg gtttcaccgc cacaccgtca cccaccgctt
     1921 tgctggcgaa tgcttcgtca ggaacctgat ccagtgccac gacatcaccg gtaatcggcg
     1981 ataccagctc cgcgatagat accgcgtttg gtacagcctg cggttttgct acaggcgcgg
     2041 cagttgacgg agttgcttca gctgacgcag cggctaccgg accacgggca acgactttct
     2101 tcatcgcatc gccgatggat tctgctttcg cgccaacaat cacctgaata gtctgtttgt
     2161 tcagtttcac tacaccagaa gcacccagac gtttacacat cgtatcgttg acgcgggcag
     2221 agtcagccac ggtcaggcgc agacgggtga tacaggcgtc aattgctttc aggttgtcag
     2281 tgccgccaac cgcagcaata tagttggtcg ccagttgatt gaggccttct tcagtgttgc
     2341 tgttggcttc ttcagtaacg atctcatctt ctttatcttc acgacccggc gttttcaggt
     2401 tgaacatgcg gataaccaaa ctgaacacca cgaagtagat agcgaagaag acaacaccca
     2461 tcaccagcag catccagacg ttctggctgg cggccggcag gttatacatc aacgcgtagt
     2521 cgatagcccc cgcagagaaa gagaagccag catggatacc cagcagtgtt gccacaaaca
     2581 ggctgatacc ggtcagcagt gcgtgcagga ggtacagcag cggtgcaagg aacatgaaca
     2641 ggaattccag cggctcagtc acaccggtca ggaacgcagt aacagcaaca gaaagcagca
     2701 tcccgccaac catcggacga cgctctttcg gtgctgcgaa gtacatcgcc agcgccgcac
     2761 ccggcagacc gaacatcata atcgggaaga agccggacat gaacatcccc gcggtgccgt
     2821 caccggcata gaagcggttg atatcaccgt ggaaaaccgt acccgccgcg ttggtgaatt
     2881 caccaatctg gaaccaggcg atggtgttca gcacctgatg cagaccggtt gggatcagca
     2941 gacggttgat gaaaccaaag ataccggaac ccagcgcgcc cgcagaaacg atccactcgc
     3001 ctcctgcatg gatagcgtgc tgtaccggcg gccagacgta accaaaaatg gccgccagca
     3061 ccaggcagaa gaagccggtg gcaatcggca caaagcgttt gccgccgaag aagctcagga
     3121 agtccggcag tttaatatcg gaccaacggt tataggctgc gccaccaacc agaccggtaa
     3181 tgatacccgc cagtacaccc atgtttattt ctgggttgat ggtcaccatc gctttggtta
     3241 acacaaagta acctaccgca cccgccagcg ccgccgcacc tgcgctgtct ttcgaccagc
     3301 tggatgccac accgatggcg aagattaatg cgaggttatc aaaaatcgca ccgcccgcct
     3361 gggcaataaa cgcaacgtta agtaaatctg gctgaccgaa tcgcagcaac agtgccgcca
     3421 ccggcagcac cgcgataggg agctgtaacg ccctaccgag tcgctggaaa aaacctaaaa
     3481 tattcatctt attcccccta cgagaaccct atttggctcg tttaaagccg tatttttatt
     3541 ttgctgcaaa ttgtactgcc gatgttctgt aatcagattg ttagatcatc tgctacagag
     3601 tgtgtgaaaa tttaattcgt atcgcaaatt aaacgcgtgt cttttgtgag ttttgtcacc
     3661 aaatatcgtt atcatcactc ccttttactg gctaaaccag aaaacttatt ttatcattca
     3721 aaaaatcagg tcggattgac gcctgtctgc gcaaatccaa gttacgctta aagatgccta
     3781 atccgccaac ggcttacatt ttacttattg aggtgaataa tgagactgat ccccctgact
     3841 accgctgaac aggtcggcaa atgggctgct cgccatatcg tcaatcgtat caatgcgttc
     3901 aaaccgactg ccgatcgtcc gtttgttctg ggcctgccaa ctggcggcac gccgatgacc
     3961 acctataaag cgttagtcga aatgcataaa gcaggccagg tcagctttaa gcacgttgtc
     4021 accttcaaca tggacgaata tgtcggtctg ccgaaagagc atccggaaag ctactacagc
     4081 tttatgcacc gtaatttctt cgatcacgtt gatattccag cagaaaacat caaccttctc
     4141 aacggcaacg ccccggatat cgacgccgaa tgccgccagt atgaagaaaa aatccgttct
     4201 tacggaaaaa ttcatctgtt tatgggcggt gtaggtaacg acggccatat tgcatttaac
     4261 gaaccggcgt cttctctggc ttctcgtact cgtatcaaaa ccctgactca tgacactcgc
     4321 gtcgcgaact ctcgtttctt tgataacgat gtcaatcagg tgccaaaata tgccctgact
     4381 gtcggtgttg gtacattgct ggatgccgaa gaagtgatga ttctggtgct gggtagccag
     4441 aaagcactgg cactgcaggc tgcagttgaa ggttgcgtga accacatgtg gaccatcagc
     4501 tgtctgcaac tgcatccaaa agcgatcatg gtgtgcgatg aaccttccac catggagctg
     4561 aaagttaaga ctttaagata tttcaatgaa ttagaagcag aaaatatcaa aggtctgtaa
     4621 ttgttatccc tgccctggct ccttgctcag ggcaatattt tttaaaatcg ggggtcagaa
     4681 tgtatgcatt aacccagggc cggatcttta ccggccacga atttcttgat gaccacgcgg
     4741 ttgttatcgc tgatggcctg attaaaagcg tctgtccggt agcggaactg ccgccagaga
     4801 tcgaacaacg ttcactgaac ggggccattc tctcccccgg ttttatcgat gtgcagttaa
     4861 acggctgcgg cggcgtacag tttaacgaca ccgctgaagc ggtcagcgtg gaaacgctgg
     4921 aaatcatgca gaaagccaat gagaaatcag gctgtactaa ctatctgccg acgcttatca
     4981 ccaccagcga tgagctgatg aaacagggcg tgcgcgttat gcgcgagtac ctggcaagac
     5041 atccgaatca ggcgttaggt ctgcatctgg aaggtccgtg gctgaatctg gtaaaaaaag
     5101 gcacccataa tccgaatttt gtgcgtaagc ctgatgccgc gctggtcgat ttcctgtgtg
     5161 aaaacgccga cgtcattacc aaagtgaccc tggcaccgga aatggttcct gcggaagtca
     5221 tcagcaaact ggcaaatgcc gggattgtgg tttctgccgg tcactccaac gcgacgttga
     5281 aagaagcgaa agccggtttc cgcgcgggga ttacctttgc cacccatctg tacaacgcga
     5341 tgccgtatat taccggtcgt gaaccgggcc tggcgggcgc gatcctcgac gaagctgaca
     5401 tttattgcgg tattatcgct gatggcctgc atgttgatta cgccaacatt cgtaacgcta
     5461 aacgccttaa aggcgacaaa ctgtgtctgg ttaccgacgc caccgcgcca gcaggtgcca
     5521 acattgaaca gttcattttt gcgggtaaaa caatatacta ccgtaacgga ctttgtgtgg
     5581 atgagaacgg tacgttaagc ggttcatcct taaccatgat tgaaggcgtg cgtaatctgg
     5641 tcgaacattg cggtatcgca ctggatgaag tgctgcgtat ggcgacgctc tatccggcgc
     5701 gtgcgattgg cgttgagaaa cgtctcggca cactcgccgc aggtaaagta gccaacctga
     5761 ccgcattcac acctgatttt aaaatcacca agaccatcgt taacggtaac gaggtcgtaa
     5821 ctcaataaga gaaagtatga caccaggcgg acaagctcag ataggtaatg ttgatctcgt
     5881 aaaacagctt aacagcgcgg cggtttatcg cctgattgac cagtacgggc caatctcgcg
     5941 gattcagatt gccgagcaaa gccagcttgc ccccgccagc gtaaccaaaa ttacgcgtca
     6001 gcttatcgaa cgcgggctga tcaaagaagt tgatcagcag gcctccaccg ggggccgccg
     6061 cgctatctcc atcgtcaccg aaacccgcaa tttccacgca atcggcgtac ggcttggtcg
     6121 tcacgacgcc accatcaccc tgtttgatct cagcagcaaa gtgctggcag aagaacatta
     6181 cccgctgccg gaacgtaccc agcaaacgct ggaacatgcc ctgttgaatg ccattgctca
     6241 gtttattgat agctaccagc gcaaactgcg cgagctgatc gcgatttcgg tgatcctgcc
     6301 agggcttgtt gacccggaca gcggcaaaat tcattacatg ccgcatattc aggtagaaaa
     6361 ctgggggctg gtagaagctc tggaagaacg ttttaaagtg acctgtttcg ttggtcacga
     6421 tatccgtagt ctggcgctgg cggagcacta cttcggtgca agtcaggatt gcgaagactc
     6481 cattctggtg cgtgtccatc gcggaaccgg ggccgggatt atctctaacg ggcgcatttt
     6541 tattggccgc aacggtaacg tcggtgaaat tggccatatt caggtcgaac cgctgggtga
     6601 acgctgccac tgcggcaact ttggctgcct ggaaactatc gccgccaacg ctgccattga
     6661 acaacgggtg ttgaatctgt taaagcaggg ctaccagagc cgcgtgccgc tggacgactg
     6721 caccatcaaa actatctgca aagccgcgaa caaaggcgat agtctggcgt cggaagtaat
     6781 tgagtatatc ggtcgtcatc tgggtaaaac catcgccatt gctatcaact tatttaatcc
     6841 gcaaaaaatt gttattgccg gtgaaatcac cgaagccgat aaagtgctgc tccctgctat
     6901 tgaaagctgc attaataccc aggcgctgaa ggcgtttcgc actaatctgc cggtggtacg
     6961 ttctgagctg gatcaccgct cggcaatcgg cgcttttgcg ctggtaaaac gcgccatgct
     7021 caacggtatt ttgctccagc atttgctgga aaattaatgt gcttttatag tggcgcttat
     7081 tgttgtcaat attctgggta gtccatgacc attaaaaatg taatttgcga tatcgacggc
     7141 gtgctgatgc acgataacgt cgccgtaccg ggtgcagcgg aatttttgca cgggattatg
     7201 gataaaggcc tgccgctggt gttgctgacc aactatcctt cgcagactgg gcaagatctg
     7261 gcgaaccgct ttgccaccgc aggtgtcgat gtacctgaca gcgtgtttta tacctctgcg
     7321 atggcgactg ccgatttcct gcgtcgccag gaaggcaaga aagcgtatgt ggtgggcgaa
     7381 ggcgcactga ttcatgaact gtacaaagcc ggtttcacta ttaccgatgt gaaccctgat
     7441 ttcgtgattg ttggcgaaac gagttcctac aactgggaca tgatgcataa agcagcctat
     7501 ttcgtcgcta acggtgcacg ttttatcgcc accaatccgg acacccacgg gcgcggtttt
     7561 tatcccgcgt gtggcgcgtt gtgtgcaggc atcgaaaaaa tctccgggcg taaaccgttc
     7621 tatgttggta aaccgagtcc gtggatcatc cgcgcggcat taaacaaaat gcaggcgcat
     7681 tcggaagaaa cggtgattgt cggcgataac ctgcgtaccg atattctggc gggcttccag
     7741 gcaggtctgg aaacgattct ggtgctttct ggtgtttcat cgctcgacga tatcgacagt
     7801 atgccgttcc gcccaagctg gatttacccg tcggtcgctg aaatcgacgt tatctgaata
     7861 gcaaccacgt ctgcggacgt ggttggctct gatgacaaac gccaaactgc ctgatgcgct
     7921 acgcttatca ggcctacgca gctcctgcaa tatattgaat tttcatgctt ttgtaggccg
     7981 gataaggcgt tcacgccgca tccggcatga acaaattgca ctttgtcagc aatttggccc
     8041 ccgtaaaata caaacattca ataaaacccg ctgaaaatta tcgattcatc aataaatata
     8101 tcctcacacc agaccttttt tcaccaatcg acaatcacca ttacgttttt tattttttca
     8161 ccgccaaatc gcttgcgcac agtgctgatt tgcggcattt ttttaaacaa gcaaacacaa
     8221 caagcaacaa ataccagatt aacggagaag gttatgtgtt caatttttgg cgtattcgat
     8281 atcaaaacag acgcagttga gctgcgtaag aaagcactcg agctgtcacg cctgatgcgt
     8341 catcgtggcc cggactggtc cggtatttat gccagcgata acgccattct cgctcacgaa
     8401 cgtctgtcaa ttgttgacgt taacgcgggg gcgcaacctc tctacaacca acaaaaaacc
     8461 cacgtactgg cggtaaacgg tgaaatctac aaccaccagg cattgcgcgc tgaatatggc
     8521 gatcgttatc agttccagac agggtctgac tgtgaagtga tcctcgcgct gtatcaggaa
     8581 aaagggccgg aatttctcga cgacttgcag ggcatgtttg cctttgccct gtacgacagc
     8641 gaaaaagatg cctacctgat tggtcgcgac catctgggga tcatcccact gtatatgggc
     8701 tatgacgaac acggtcagct gtatgtggcc tcagaaatga aatcgctggt gccagtttgc
     8761 cgcacgatta aagagttccc ggcggggagc tatttgtgga gccaggacgg cgaaatccgt
     8821 tcttattatc atcgcgactg gttcgacttt gatgcagtga aagataacgt gaccgacaaa
     8881 aacgagctgc gtcaggctct ggaagattcc gttaaaagcc atctgatgtc tgatgtgcct
     8941 tacggtgtgc tgctttctgg tggtctggat tcctcaatta tttccgctat caccaagaaa
     9001 tacgcagccc gtcgcgtgga agatcaggaa cgctctgaag cctggtggcc gcagttacac
     9061 tcctttgctg taggtctgcc gggttcaccg gatcttaaag cagcccagga agtggcaaac
     9121 catctgggca cggtgcatca cgaaattcac ttcactgtac aggaaggtct ggatgccatc
     9181 cgcgacgtga tttaccacat cgaaacttat gatgtgacca ctattcgcgc ttcaacaccg
     9241 atgtatttaa tgtcgcgtaa gatcaaggcg atgggcatta aaatggtgct gtccggtgaa
     9301 ggttctgatg aagtgtttgg cggttatctt tacttccata aagcgcccaa cgctaaagaa
     9361 ctgcatgaag agacggtgcg taaactgctg gccctgcata tgtatgactg cgcgcgcgcc
     9421 aacaaagcga tgtcagcctg gggcgtggaa gcacgcgttc cgttcctcga caaaaaattc
     9481 cttgatgtgg cgatgcgcat taacccgcag gataaaatgt gcggtaacgg caaaatggaa
     9541 aaacacatcc tgcgtgaatg ttttgagtca tacctgcccg caagcgtggc ctggcggcag
     9601 aaagagcagt tctccgatgg cgtcggttac agttggatcg acaccctgaa agaagtggcg
     9661 gcgcagcagg tttctaatca gcaactggaa actgcccgct tccgcttccc gtacaacacg
     9721 ccaacctcaa aagaagcgta tctgtaccgg gagatctttg aagaactgtt cccgcttccg
     9781 agcgccgctg agtgcgtgcc tggcggtcct tccgtcgcgt gttcttccgc taaagcgatc
     9841 gaatgggatg aagcgttcaa gaaaatggac gatccatctg gtcgtgcggt tggtgttcac
     9901 cagtcggcat ataagtaaga aatgcgaaac agccccggtg aaatccgggg ctgtttcagt
     9961 tattgaggtg cttgttgatt ctgatatgca gtaaggaaat gttccgggtt tgccacatca
    10021 ctgtttgaca actcatcact gtaggccagt atttcataga ttaatttccc accactattg
    10081 ttgaattccg aatttcatcc tgtacgacaa aagaatggct gtagggtggg gaaaaaacac
    10141 aaagtaaaca ataattgacg aatatagcgc cacgctgttc gcaacctaac caaatagtca
    10201 ctttcgagca attttccttg aaaaagaggt tgacgctgca aggctctata cgcataatgc
    10261 gccccgcaac gccgataagg tatcgcgaaa aaaaagatgg ctacgtagct cagttggtta
    10321 gagcacatca ctcataatga tggggtcaca ggttcgaatc ccgtcgtagc caccatcttt
    10381 ttggcgggag tggcgaaatt ggtagacgca ccagatttag gttctggcgc cgcaaggtgt
    10441 gcgagttcaa gtctcgcctc ccgcaccatt caccagaaag tgttgtacgg atggggtatc
    10501 gccaagcggt aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc
    10561 cagccatctt cttcgagtaa gcggttcacc gcccggttat tggggtatcg ccaagcggta
    10621 aggcaccggt ttttgatacc ggcattccct ggttcgaatc caggtacccc agccatcgaa
    10681 gaaacaatct ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca
    10741 caggttcgaa tcccgtcgta gccaccaaat tctgaatgtg tcgaatacgt tcggcaaatt
    10801 caaaaaccaa tttgttgggg tatcgccaag cggtaaggca ccggattctg attccggcat
    10861 tccgaggttc gaaccctcgt accccagcca atttattcaa gacgcttaca tcgtaagtgc
    10921 acccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
    10981 tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc
    11041 tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga
    11101 cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca
    11161 accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctct gccgccatta
    11221 acccgagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca
    11281 gatccatacc actttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga
    11341 ggacaggata actggcccac gcttcaccgt aactgcgggc attcaccaga acatcaatca
    11401 gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat
    11461 gcgcggcatc gcccaccagc gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg
    11521 tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct
    11581 tcgcgatttc cgcctggagt tgcgccatat tcatattctg caactggcga atacgcgccg
    11641 gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc
    11701 cgtccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa
    11761 tcaacatgca cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgccgca
    11821 cctgagaatt tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt tcaccgccct
    11881 tcagttccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag cctggcacac
    11941 gtaacgttac tttcggatgc gcttccagcg cctgccacag cgcctgttgc aggacggtgt
    12001 tttccaccat atagccaagc aacggtagct taagttcagc ggcgtcaaac accacatgcg
    12061 ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg
    12121 catcccagac ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc gccgagatcc
    12181 gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta
    12241 ccgaaaatcc gtgctgtgcc agccccagcg ccagcgcgcc gccgaccatt cctccgccga
    12301 caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt
    12361 aagtttacag gatttttacc cccaagggct gataacgctc atactggcca caacggcagc
    12421 aaagcattac actatgcgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga
    12481 ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga
    12541 tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg
    12601 atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc
    12661 agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg
    12721 gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta
    12781 tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc
    12841 gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac
    12901 cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca
    12961 cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc
    13021 tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga
    13081 acgtgaacgc ctggcgtggt gaaaactacg acggctccac cggatcgttt gccgatctgc
    13141 tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga
    13201 tcgaattcac cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc
    13261 tgcatctgcc ggtacagagc ggttccgatc gcattctgaa cctgatgggc cgtactcata
    13321 cggcactgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtcca gatattcaga
    13381 tcagttctga tttcatcgtt ggcttcccta gcgaaaccac cgaagacttc gagaaaacga
    13441 tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc
    13501 cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag aagcagcgtc
    13561 tgtatattct gcaagaacgt atcaatcagc aagcgatggc atggagccgc cggatgctcg
    13621 gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg gagctttccg
    13681 ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat
    13741 tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta
    13801 ctgaagatga aatgggcctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc
    13861 gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc
    13921 ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc
    13981 ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga
    14041 taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc
    14101 gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct
    14161 cggcatcgag atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt
    14221 caccgctgcg gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca
    14281 gattcaggat atcgaaccgg aacagatcca ccttacgatt aaagaagcgc gagtactgga
    14341 gcaaagtgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg
    14401 cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga
    14461 cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc
    14521 agttgatgcc ctggagcgtc aggagattcg ccgtattctg ctgactcgtc cggcggtcga
    14581 agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct
    14641 gcgcccactg tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg agaaactgat
    14701 tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga
    14761 cgcgtttatc attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct
    14821 gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct
    14881 gccgcgtaat actaaatcag gcttacgtca cgccatcgaa gtactggccg atgtcgaaga
    14941 gatcagcttt aacttcttcc acagcgaaga cgtggttcgt caccctgtgg tggcgcgtat
    15001 cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg cgctggcggc
    15061 agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg
    15121 gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcaaacatg gctgaatgcg
    15181 gtgatcccgc agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa
    15241 agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc
    15301 ccgtttgaag tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt
    15361 caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat
    15421 atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca
    15481 gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac
    15541 attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga
    15601 ttaacgacgg cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca
    15661 attcacacag tagtgacact ataagcaaca agaagggatt tttctccctg ttactcagcc
    15721 aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg
    15781 ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg
    15841 cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca
    15901 accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg
    15961 tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt
    16021 ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg
    16081 ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca
    16141 tggcgatcgt cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc
    16201 tggaactgat tgttggtgag attgaagacg aatatgacga agaagatgat atcgacttcc
    16261 gtcagctgag tcgtcatacc tggaccgtgc gcgcactggc ttccattgaa gacttcaacg
    16321 aagcgttcgg cacccacttt agcgatgaag aagtcgacac tatcggtggt ctggtgatgc
    16381 aggcgtttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca
    16441 aagtggcgat ggccgacagt cgacgtatta ttcaggttca tgtcaaaatc ccggatgact
    16501 caccccagcc gaagctggat gaataaaacc gaaactggat agataactac atggcttttg
    16561 cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg
    16621 gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg ttgatggggc
    16681 ttcaggcgtt gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg
    16741 gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg
    16801 gaatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt
    16861 ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg
    16921 tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga
    16981 caggcttccc gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg
    17041 caccgataat gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg
    17101 cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc
    17161 tacccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg
    17221 tttcgatggt tcagggcgat attccgcaat cactgaaatg ggatggagat cagcttctta
    17281 atacgctgaa gatttactac aacgcaacgg ctccgctgat gggcaaatca tcgttgatta
    17341 tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac
    17401 tggacggtga gttgcgcgat aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc
    17461 tcaataagca gaaccgctac gatacctaca acaccatcat cacactgggt aaaggtgcac
    17521 cgtacagcta cgaatcagcc gatcgctata acaaaaacca tctggtgccg tttggcgagt
    17581 ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt
    17641 cgttcagccg tgggccatat atccagccgc cgctgtcagt aaatggtatt gagcttactg
    17701 cggctatttg ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata
    17761 ccgactatct gctgactatc tccaacgatg cgtggtttgg taaatctatt ggtccatggc
    17821 aacacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca
    17881 ccaacaacgg cattacggcg gtgattggcc cgcagggtga gattcaggcg atgatcccgc
    17941 agttcacccg cgaggtgtta accactaatg tgacaccgac caccggactc acaccgtatg
    18001 cacgtaccgg caactggcca ctgtgggtgc tgacggcact gtttggtttt gccgctgtgt
    18061 tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg
    18121 tactttctca ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga
    18181 tacaagccac attgccatat tggggatgac gaataattgc ctgcggatca agcgagcgcg
    18241 tcctgtccat ttggatcaac ccgttcttcg ccatgac
//
LOCUS       DAJTOR010000076        17514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTOR010000076 DAJTOR010000000
VERSION     DAJTOR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.076"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..744
                     /locus_tag="OO249_002264"
     CDS             <1..744
                     /locus_tag="OO249_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5636545.1"
                     /translation="SSTDKPAKLKFHQYARATLLSNRGRDHNCDGRRTVEIHKLDLSD
                     WQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLL
                     QDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYI
                     DQLHTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRD
                     IRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(815..1264)
                     /gene="rplI"
                     /locus_tag="OO249_002265"
     CDS             complement(815..1264)
                     /gene="rplI"
                     /locus_tag="OO249_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5636546.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="OO249_002266"
     CDS             complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="OO249_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5636547.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1538..1852)
                     /gene="priB"
                     /locus_tag="OO249_002267"
     CDS             complement(1538..1852)
                     /gene="priB"
                     /locus_tag="OO249_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5636548.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="OO249_002268"
     CDS             complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="OO249_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5636549.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2581..2856
                     /locus_tag="OO249_002269"
     CDS             2581..2856
                     /locus_tag="OO249_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5636550.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2931..3482)
                     /locus_tag="OO249_002270"
     CDS             complement(2931..3482)
                     /locus_tag="OO249_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR5636551.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(3579..4265)
                     /gene="ulaF"
                     /locus_tag="OO249_002271"
     CDS             complement(3579..4265)
                     /gene="ulaF"
                     /locus_tag="OO249_002271"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5636552.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4265..5119)
                     /gene="ulaE"
                     /locus_tag="OO249_002272"
     CDS             complement(4265..5119)
                     /gene="ulaE"
                     /locus_tag="OO249_002272"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5636553.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="OO249_002273"
                     /pseudo
     CDS             complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="OO249_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
     gene            complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="OO249_002274"
     CDS             complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="OO249_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5636554.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="OO249_002275"
     CDS             complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="OO249_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5636555.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="OO249_002276"
     CDS             complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="OO249_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5636556.1"
                     /translation="MSAQHSGCAEYPVTSSSGDRLYNIFTVFFNQVMTNAPLLLGIVT
                     CLGYILLRKSVSVIIKGTIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISD
                     TYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGL
                     IAVTLFIFGYSMWTTIICTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASW
                     IAYKVAPFLGKKEESVEDLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAM
                     AGKVNWTVYILQTGFSFAVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAA
                     IYSFAPNAVVWGFMWGTIGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFG
                     GWRAALKICLVMGMIEIFGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMA
                     VIIVIALAYMFFAGRALRAEEDAEKQLAEQSA"
     gene            8340..9404
                     /gene="ulaG"
                     /locus_tag="OO249_002277"
     CDS             8340..9404
                     /gene="ulaG"
                     /locus_tag="OO249_002277"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5636557.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVQWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9512..10267
                     /gene="ulaR"
                     /locus_tag="OO249_002278"
     CDS             9512..10267
                     /gene="ulaR"
                     /locus_tag="OO249_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5636558.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="OO249_002279"
     CDS             complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="OO249_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5636559.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11195..11524
                     /gene="bsmA"
                     /locus_tag="OO249_002280"
     CDS             11195..11524
                     /gene="bsmA"
                     /locus_tag="OO249_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5636560.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11673..11948
                     /gene="yjfN"
                     /locus_tag="OO249_002281"
     CDS             11673..11948
                     /gene="yjfN"
                     /locus_tag="OO249_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5636561.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12065..13690)
                     /locus_tag="OO249_002282"
     CDS             complement(12065..13690)
                     /locus_tag="OO249_002282"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5636562.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSLLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13774..14832)
                     /locus_tag="OO249_002283"
     CDS             complement(13774..14832)
                     /locus_tag="OO249_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5636563.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
                     QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQW
                     VEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGN
                     VTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIRED
                     NTLITKDTSRFVPHYIAG"
     gene            complement(14835..15473)
                     /locus_tag="OO249_002284"
     CDS             complement(14835..15473)
                     /locus_tag="OO249_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5636564.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWLNTSGDYSWRSGSGKKESYSSGGFTTKKASTISRGGYGRSSSARGHWGG"
     gene            complement(15483..15881)
                     /locus_tag="OO249_002285"
     CDS             complement(15483..15881)
                     /locus_tag="OO249_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5636565.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15899..16558)
                     /locus_tag="OO249_002286"
     CDS             complement(15899..16558)
                     /locus_tag="OO249_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCR5636566.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16609..17307)
                     /locus_tag="OO249_002287"
     CDS             complement(16609..17307)
                     /locus_tag="OO249_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCR5636567.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
     gene            complement(17380..>17514)
                     /locus_tag="OO249_002288"
     CDS             complement(17380..>17514)
                     /locus_tag="OO249_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCR5636568.1"
                     /translation="ITRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEIT
                     "
BASE COUNT         4470 a         4678 c         4188 g         4178 t
ORIGIN      
        1 tcgtccaccg acaaaccagc aaaattaaaa tttcatcaat acgcacgcgc tacattactg
       61 tcaaatcgtg gaagagatca taattgcgat ggcagacgta ctgttgagat ccacaaacta
      121 gatctctctg actggcaagc attcaataag ttagcaacga agtgtaatgc ttacgatgga
      181 gtaacaatga atggtgataa ttcttttgga tggaaccatg aagctacatt agataatatt
      241 cacgcccaga aatacaataa agcttacgcg ggtgcgaggt taaccgcaga actgaaatat
      301 ttattgcaag atgtcgaatc cttcgaaccc aacagtaaat acacaataca cgaagtggtt
      361 ttgggacctg gttatggtac tccagactat accggccaaa cgataggata cgttgtaacc
      421 cttcctgccc aaatgcctaa ttgctggtca tctgaactac caacaatcga tctttatatc
      481 gaccaacttc acactgttac aggcgtttct aatgccctcg gattcatcat tgctgcattg
      541 ctaaacgcgt attctgattt accccatgat ttgaaaattg gtctgcgttc cttatcaaat
      601 tcagcagcaa tctactcggg tttaggtttc gagcgagtgc cgcaggaccg agacataagg
      661 agtgatcgta tgcatctaac gccagcaaac catcctgact tatggacaca agagaatggt
      721 gaatggatct acttgcgaaa ctaatacctg acgaaaaata gaaagcaaaa cgccgaccaa
      781 tggtcggcgt ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca
      841 ctttcgcgaa tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca
      901 gaacgccgtt cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag
      961 cgtcagcgat gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag
     1021 acgcgatggt aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca
     1081 gaacttcagc cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt
     1141 tcttggtagc tggaacagct ttaccctgcg gtaccaggaa gttacgagca tagcccgctt
     1201 taacgtttac ctgatcacct aggctaccca ggtttgctac tttatcaagc agaataactt
     1261 gcattacctt atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc
     1321 agtgtacggc agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg
     1381 gtattttgca cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta
     1441 gtttttcagc gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg
     1501 gcagaacttg cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt
     1561 caatctgctc ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa
     1621 tgaacccctg aacggttatg cgactgccga ccgttatact gtgagtaatg gcctggtttt
     1681 cgtgtccgct aacaataacg ggcatttggc accacgcctg ccggtgaaaa ccggcttcct
     1741 cctgcacaga acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct
     1801 ttcgaagggg agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt
     1861 actcttcaga atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac
     1921 gctcacggcg ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg
     1981 tacgcataac catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat
     2041 cgatcacttc ctgcggagct tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt
     2101 tgatcgggta agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg
     2161 caccagtgat ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag
     2221 gatggaccat aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag
     2281 cctcctgtct gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa
     2341 aaatgacgcg ttatagtact tgtatgcccc actaaactca aggccagccg acaaataaat
     2401 ctcatctttc gctcaaaaga actaactcgc tgatttgaag ttcatcacat tccgtcacag
     2461 tgttttttcg aacaactgca tcagaaatgg tcaccatcaa cttctttatc attgattaaa
     2521 attaaatcag aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct
     2581 atgttcagtc gtgttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca
     2641 gccattgaaa ttaataacca ccaggccaga aatatggacg acgtacagag cttaggcgtg
     2701 atttatatca atcataattt cgccactgaa agcgaagcac gtcaggcatt aaatgaagag
     2761 acagatgcgc agggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac
     2821 ggcaatatgc acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat
     2881 tgccatagtt tgctttgccc ccttcgcagg gggctttttt atacggttat tcaccggatt
     2941 gcgacttccg gatctggctc atgagtaatg cgattacgta agtcgcggcg cataatttct
     3001 attatccaga accagaaaat atgccccagg atttcagagg catattcgtc aaaaggcaat
     3061 tgagtgagcg gcggcgttaa attcagcgcc gggcaaaata taccgtggac tgccactgtc
     3121 acaataagcc ctgctaatat cccctgccat aatttcactt tcgggaaaat ttccgcgaca
     3181 atacaatacc ctatcgcaaa aaccagtgag aagatgatat gcgtcaccat caccgggttg
     3241 ataatatgtt ccgagaatgt atacacagtt tgggttgggt cgatacctaa ataatcccgt
     3301 agaaagatat acggcgggtt aaattcatcg cgaccaccgg aaaatgtgcg cggtggcaag
     3361 gggacttccg ctccccattt aacaaatgct gaaataattc ctgaaagtac acccacaagt
     3421 aacgcaacgc catagcggcg acgttgcgga tccgttctga caaacaatcc attgatagcc
     3481 ataaaaacac ctgttgttta tacgattttt taactgaagc ggccccggaa tcagaccaat
     3541 tacatcccgc aggaggtaat aattccgggg cgcactgctt acttctgccc gtaataggcg
     3601 ttaggaccgt gcttacgcat gaagtgttta ttcatgagga agctgtcgat gtgattcagt
     3661 tgtgggttaa tactgcgcgc aatccacgcc attttcgcca cttcttccat caccaccgcg
     3721 ttatgcaccg cgtcgtgggc atctttcccc caggcgaacg gcccgtgctg atacaccaca
     3781 attcccggcg tatgcagcgg ctcggcgtca cccagcgttt cgataatcac tttgccggtg
     3841 ttcagttcat actcgccctg cacctcttct tcgcttaatc cacgcgtaca cgggatgtcg
     3901 ccaaagaagt agtcggcgtg cgtggtgcct aacgccggga tcgccagccc cgcctgcgcc
     3961 catgcggtgg catgagtgga atgggtatgg acaatgccgc cgagcgacgg gtaacgacgg
     4021 tagagttcaa gatgcgtcgc ggtgtcagaa gacgggcgat acttcccttc taccaccttg
     4081 ccgctcatat cgaccaccac catatcgtct gctttcatgg tttcgtaggc aacgccgctg
     4141 ggcttgatca ccaccagccc gcgttcgcgg tcgatagcgc tgacgttgcc ccaggtaaag
     4201 gtcaccagcc catagcgcgg cagatccatg ttggcttcaa acacctgctg ttttagcttt
     4261 tgcattatgc cgcctccacc atgcctgctt tggccatgcg cgctttcacc caatcacgcg
     4321 ctttcaccac ttctgccgcc gggtcttccg ccgtttcgct ccacatctca atcaggtacg
     4381 gcccgcaata gccactctgt ttgagcgttt cgaagcaacg ttcgaaatcc accacacctt
     4441 cgccaaatgg cacgtttttg aagacgcccg gtttggtgtc tttcacatgc accgcgacga
     4501 tatgcccgat tccggcctgc aactccatct gcacatcgtt gtcccacgcc gacaggttgc
     4561 cgatatctgg gtagagctgg aaccacgggt tgttgagata gtgcgcgtaa cccagcgcct
     4621 tgctgatgga gttcatcaac ggataatcca tgatctccat cgccagcgtc acctgcgcgc
     4681 ggctcgccat ctcaacgctc tctttcaggc cgtcacggaa acgacgacgc gtttcgttat
     4741 tggcttcctg atagtaaacg tcatagcccg ccagctggat cacgcgaatg ccaacgtcct
     4801 gggcgaactg gatggcttta cgcataatct ccagcccctg cgcccgcacc gcgtcatctt
     4861 cgctgcccaa cgggaaacga cgatgggcag aaaggcacat ggacggcaca cgcacgccgg
     4921 tttcaacaat cgcattcacc agcgccagac gctgctcgcg gctccagtcg aggcgcgaca
     4981 ggcgctcgtc agtttcatct accgacatct cgacaaaatc gaagcccaac gtttttgcca
     5041 gtcgcaggcg ttccagccag cactccccgg cggggagcgc tttttcatag atgccaagcg
     5101 ggatttgttt ggacaacata tccgctcctt agccccacag ttcggcgatg gaacgtttga
     5161 actgacgcgc ggcttcaacc ggagaagcgg catcacggat gctgcgaccg gcgataaaca
     5221 catgaattgg aatgcctttg aacagcggca gatcttccag cgccaggcca ccggtgacgg
     5281 tgactttgaa gcccatatcg gaaagacgtt tgatcgcagt gatatcggct tcaccccacg
     5341 ccacgccagc ggcctgcgcg tcacggctgc ggtgataaac cacctgctga atacctgcgt
     5401 cgcgccactg ttacgcctgt tcccaggtcc agtaaccggt cagttcgatc tgcacgtcgc
     5461 cgttaaactc ttttgctacg tccagcgcac ctttggcggt gttgatatcc gcacaacaaa
     5521 tcacggtcac ccagtcagcg ttggcttcga agcacatacg agaaagaatt ttgcccgcat
     5581 cggcaatttt ggcgtctgcc agcacgattt tgtgcgggta gagcgctttc agatcgcgaa
     5641 ccgcacgcac gccttcgccc acgcacagaa tggtacccac ttcgataatg tcgacttctt
     5701 cggcaatcag gcgagtggtt tcgtaggcgc tatccatagt ctggttgtcc agcgcgactt
     5761 gcaacatcgg taatgacatc ttcaattcct tcttaagctg ccgcgttggt gcggtcaatg
     5821 agatccagta cttcctgctc ggtacggcag gcacgtaaac ggtcgaaatt ttcttcatct
     5881 tcaaacaggt tgacgatctg catgatgccc acttcctggt gcgtgttggc atcgaccgcc
     5941 gccatggtga tgaggatatc caccgggtcg ttatcttcgt ggttgaactc cagcggcttt
     6001 ttcagcgtca ccagcgcaaa accggttttc ttaacgccct cttccgggcg accgtgcggc
     6061 attgccaggc ctggtgcgag cacaaagtac ggaccgtgct gttcaaccgc atccagaatc
     6121 gcctggtagt aacgcggctc gacaacatcc gccgccacca gcaggtcaac gccgattttt
     6181 actgcgtcct gccatgtctc agcttcagcc tgcaggcgga tggatttatt ctccgccagc
     6241 gaatcacgta atttcatggc gcgtccttac ttcacatcct gcgggaaatg cgctttgatc
     6301 acttccagca gtttcgggcc aaagtcggca ggagagagca tgttgcgcac gccaaccacg
     6361 tatttgttgc cggtcacggt gatttcgccc gcaatgtgcg tagaagcgat gatgatatcc
     6421 gcgccgctca actcgctttt gtattcgcca accgcgcagc tatttaccgt gtggtcaatg
     6481 tttgattggg ttaaaaactg gtccactttc atcttcatga tcatggaact gccttgtccg
     6541 ttgccacaca cagccagaat acgtacggtc ataatcaaaa ctccttatta agcagactgt
     6601 tctgccagtt gtttttctgc atcttcttct gcgcgcagtg cgcggccagc gaagaacata
     6661 taagccagtg caattacaat gatgacggcc ataaaggcga taccgatgga gaagaagccc
     6721 tgcatcatcg gcggtgccag aatcgaccag tccgccatgc ccatccaggc gctcataccg
     6781 gtgagtttca ccgcccagac gcagccaaag atttcaatca tccccatcac cagacatatc
     6841 ttcagcgccg cacgccagcc gccgaagtgg ttagcgaaca cgccgatggt ggcgttggag
     6901 aagaacatcg ggataaagcc gggaataatc aggatcgagg aaccgcaggc gaccaggatg
     6961 ccaaccgcaa tcagctgacc gatggtgccc cacataaagc cccaaaccac ggcgttcggc
     7021 gcgaagctat agatagccgc acagtcaatc gccagaaccg caccagggat caggcgctgg
     7081 gagataccgt taaatgcttc agagagttcc gccacaaaca tgcgcacgcc ctgagtgatg
     7141 atgaagatcg ccaccgcgaa ggagaaacca gtttgcagga tgtacaccgt ccagttcact
     7201 ttgcctgcca tcgcctgcac ggtgtcgata ccgaaggaga gtagaatggc accaaagaag
     7261 atggtcatca caatcgccgt ggagacgatg ttgtcgtgga aaatattcag ccagcctggc
     7321 agtttgaggt cttcaacgct ttcctctttt ttgccgagga acggcgcaac tttataggca
     7381 atccatgatg caaactgttg ctggtgaccg atggagaaac cgcagccgtc cgtcacttcc
     7441 tgagtcggct tgtacatcat attggaggta atgccccagt agagcgaaac cagaatcgcc
     7501 gtgcagataa tggttgtcca catggagtag ccgaaaataa acaacgtaac ggcaatcagc
     7561 cccgcctgct ggaacatgat atgaccagtc agcatgatgg tacgaatacc ggtaatgcga
     7621 cgcagcagca cgtaacagat gttcagcgcc agcgctaaca acaccgcgta acctacccag
     7681 ctataggcat cgcccatgcg gtcgatggtt gccatcatcg acgcgtaagt atcggaaatt
     7741 gcgccgttaa tgccgtagac ttcggacatt ttcgccacca ccggtttgaa ggtgctggtg
     7801 aggatgccgg accctgcctg caacaacatg aaaccaatta tggttttaat cgtgccttta
     7861 ataataacgc tgacactttt gcgcagtagg atgtagccca gacaggtcac aatacccagc
     7921 aacaacgggg cattggtcat gacctggtta aaaaacacgg taaagatgtt gtagagacga
     7981 tctcctgaag aagaggtaac cgggtattcc gcacacccgg aatgttgtgc actcactcta
     8041 attttcaaaa gcaatcacaa caagattatt tatgattaaa tgtgacgcga cccgcaaata
     8101 attccatacg gatagagtgg gatccacaca acgaaacaat tatttattta aatttagtta
     8161 aattcattta gttaatttaa ctatacattc cattaaatct ttccacactc aaaatcaaca
     8221 acaacttccc tggcaaaaag tcaaaaaatg gtcattgcct tcctgaaaaa atagtgtcag
     8281 gattaatcaa aaccaatcac atattgattc gaattgaact atcatgaagg taaatggcga
     8341 tgagtaaagt gaaaagtatc acccgagaat cctggatcct gagcactttc ccggagtggg
     8401 gtagctggct gaatgaagaa attgaacaag agcaggtcgc ccctggcaca tttgcgatgt
     8461 ggtggctagg ctgcaccggg atctggttga aatcagaagg tggcaccaac gtttgcgttg
     8521 atttctggtg cggcactggc aaacaaagtc acggtaaccc gttaatgaaa cagggtcacc
     8581 agatgcagcg catggctggc gtgaaaaaac tgcagccaaa cctacgcacc accccgtttg
     8641 ttcttgatcc gtttgcgatt cgccagatcg acgcggtact ggcgactcac gatcacaacg
     8701 atcatatcga cgttaacgtc gctgctgccg tgatgcagaa ctgtgctgat gacgtaccat
     8761 tcattggacc gaaaacctgc gttgatttgt ggattggctg gggcgtaccg aaagagcgtt
     8821 gcatcgtggt caaaccgggc gatgtggtga aagtgaaaga cattgaaatt catgcgcttg
     8881 atgctttcga ccgtactgca ctgatcaccc tgcctgccga tcaaaaagcg gctggcgtac
     8941 tgccggacgg catggacgat cgcgcggtga actatctgtt caaaacgcct ggcggctccc
     9001 tgtatcacag cggcgactcc cactactcta actattatgc gaagcacggt aacgaacatc
     9061 agatcgacgt ggctttaggt tcatacggcg aaaacccgcg cggtatcacc gacaaaatga
     9121 ccagcgccga tatgctgcgt atgggtgaag ccctgaatgc gaaagtggtt atcccgttcc
     9181 accacgatat ctggtcaaac ttccaggccg ctccgcaaga gatccgcgtg cagtgggaga
     9241 tgaaaaaaga tcgtctgaag tatggcttca agccgtttat ctggcaggtt ggcggcaaat
     9301 ttacctggcc actggataaa gacaacttcg agtaccacta tccgcgcggt ttcgatgatt
     9361 gcttcactat tgaaccggat ctgccgttca aatcattcct gtaatctgtc gtttataccg
     9421 gatagcgtgg agaagttcgc gctatccggc gtagtatttc ctggctattt caaatatcat
     9481 ctaaaaaaat caaattttat cggaatagct catgactgaa gcacaaagac atcaaatcct
     9541 cctggaaatg ctcgcacaat tgggctttgt gaccgttgag aaagtcgttg agcgtctggg
     9601 aatttcgcct gccactgcgc gacgcgatat caataaactt gacgaaagcg gcaaactcaa
     9661 aaaggtgcgc aatggcgcag aagctattac ccaacagcgc ccgcgctgga cgccgatgaa
     9721 tctgcatcag gcgcagaatc acgatgaaaa agtacgtatt gctaaagcgg cctcgcagct
     9781 ggttaatccg ggcgaaagcg tagtcatcaa ctgcggctcc accgcgtttc tgcttgggcg
     9841 ggaaatgtgt ggcaagccag tgcaaatcat cactaattat ctaccgctgg caaattacct
     9901 gatcgatcaa gaacatgaca gcgtgatcat tatgggtgga cagtacaaca aaagtcagtc
     9961 catcacttta agcccgcagg gcagcgaaaa cagtctctat gccgggcact ggatgtttac
    10021 cagcggaaaa gggctgaccg cagaagggtt gtataaaacc gatatgctga cagcaatggc
    10081 agagcagaag atgctgagcg tggtagggaa actggtggta ctggttgata gcagtaagat
    10141 tggcgaacgc gcgggaatgc tttttagccg tgccgatcaa atcgatatgc ttatcaccgg
    10201 caaaaatgct aacccggaaa tcctgcaaca actggaagcg caaggggtca gcattctgcg
    10261 tgtttaaaga tgctggcgga aaaatgtcac ggcagcatct aacgcctcag gcgtaatgcg
    10321 gtgacgcacg cctggctgcc atgaacaggt tagcagttta tcccgtcccg tctcgcttaa
    10381 ggcctgctgc aaacgtagtg attcgtcggc aggcacaacg tcatcgtcga ggccatgcca
    10441 cagcagtaga ggtctgtccc caagttgttc caggtggttt gtcgcttccc actctgccag
    10501 cggcgcgata atgttattga attcattctg ctgtgctgcc gtttcaggta tcagcggtgg
    10561 aaacagtgaa cgggcgagcg atgtaaaata gcccgatccc atcatgctgg cggtacattt
    10621 caccgtgggg tggcgagcgg taattcccag tgccgtcatc gcgcccatcg acgcgccacc
    10681 gactgccaga cggtcatcaa gcagccagtt ttcttcggct attgccgcac gtaaagtagt
    10741 gaattcctgc atactttgta gcaagatttg ccagaattga tttaaccgcc gcgcttcgtc
    10801 gctactaaaa cggctaccgt gatcgggcgc atccggcatg atcacccgca aaccagcctg
    10861 cgccagcgca acggcaaaat agctatacac caggctggat gaagtaaagc cgtgataaaa
    10921 aattacgcac ggtaacgggg tatctttttg cccgacagga taagcatgaa gaacgggaat
    10981 atctgccagc tcgcgtgatt ctatttcaat catctgtctc tcctttttgt ctggtatgac
    11041 atgaagaaat ccttagcaca agtttctgct ggcggtatta gttcataatg ttgagatgtg
    11101 ggttacgctt tcgtcacgtt ttcattcgaa aatcgcgtac caggtaacaa ttcgggaaca
    11161 ttcccccaaa gttaaattaa taaggcacta cactatggtt agcaggaaac gtaatagcgt
    11221 tatttaccgg tttgccagtt tattattggt gttgatgtta agtgcctgta gcgcactgca
    11281 aggtacgcca cagccagcac cgccagtaac gaaccatccg caagagattc gccgcgacca
    11341 gacgcaaggg ttacagcgaa taggtagcgt aagcacgatg gttcggggtt ctccggatga
    11401 cgcattagca gaaatcagag caaaagctgt cgctgcaaaa gctgattatt acgttgtcgt
    11461 catggtggac gaaaccatcg tgacaggaca gtggtattca caagccattt tgtaccgtaa
    11521 ataaatcaga caacttacat agtcagcttt acattgcttt gcgtccctat gcgtttacct
    11581 gtgcacaata aattaccggc tggaagcctg ggcgtttatt accgcgatgg aatgccctgc
    11641 aacatgtggg gaaacgaaaa atggagctga cgatgaaaca attacttgct tcaccctcgc
    11701 tgcaattagt gacttatcct gcgagcgcca cggcacagtc tgcagaattc gccagtgctg
    11761 actgcgtgac gggcctgaat gaaataggtc agatctcagt tagcaatatt tcaggggatc
    11821 cgcaggacgt ggaacgtatc gtggcgttaa aggccgatga acaaggtgcg tcatggtacc
    11881 gcattattac catgtacgaa gaccagcagc ctgataactg gcgcgtacag gcgatactgt
    11941 atgcatgagt gtcatctaca gcaaatgaac aatgccggat tcggcgcgag cgccttgtcc
    12001 ggcctacaat tttcacatat ttcattgagt taatcgaaac cagcgtcgca tcagtcgtat
    12061 acgcttacac acacactccc cccgtcgccc gcagcaataa atcattctgg atctgctctg
    12121 acaaccgtac gccgccgcgc gtatctaaca tcacctgaca ccacgcctgc gccattggcg
    12181 gagaagcata tttcaacatt tgcgcaccgc agcccagcag gaatagctga tgagtaatct
    12241 ctcgccccag ttcttcagct ggcttacgca gctgctgctg taaacgacga accgcgcgat
    12301 caaaatagcg atcctgccct ttcacttcca caaatgcttc cgacaataag tcgtatacgc
    12361 ccgcttgctt attgagaacg cgcaacacat ccaggcacat aatattgccg gaaccttccc
    12421 aaatactgtt taccggcatc tcccggtaaa gccgcggcag ctcgctctcc tcgcaataac
    12481 caatgccgcc cagcacctcc atcgcttcgg ccacaaacgg catacctctt ttgcagatca
    12541 caaattttgc cgcaggcgta aataaacgcg cccacagggc ttctttggca tcggcacgcc
    12601 ggtcccacgc tcgcgcaaga cgaaacagca acgccgtttg cccttcaagc tgaagtgcca
    12661 tgcgacttaa gacatgacac ataaggggct gttggatcaa tggattacca aaaacatggc
    12721 gttgatgtgc atgataaatc gccagcgaaa atgcacggcg catcatggca tggctaccca
    12781 gggcgcaatc aaaacgcgtc atcccaccca ttttcaggat cagacgaatt ccttcccctt
    12841 cctgccccaa caaccagcca atggcatcct gaaactccac ttcgcaactg gcgttagagc
    12901 gattacccag cttatctttc agccgctcga ggcgaatcgc gttgcgttgc ccatcaggca
    12961 aaaagcgcgg cacaaaaaag caggacaaac cgcccgtagt ctgcgccagc accagatgcg
    13021 catcgctttg cggcaccgag aaaaaccatt tatgccccac cagccgataa gagccatctt
    13081 ccagacgctc tgcacgggtg gtgttgctca tgacatcgga accgccctgc ttttccgtca
    13141 ttcccatgcc aatcaacaaa ccgcgttttt gcccacctgg caataagaga gaatcgtagc
    13201 gatcgctcag cagcggcgtg gtccagtcct gaaacggcgt gggtaacatc tgtaacaaca
    13261 atggcgtggc ggcaaaggtc atcgttattg gacataacga cccagcctca acctgcgcat
    13321 gtaacataaa acgcgccgcg cgcgccacaa atgcgccgga gcgagcgtct tcttcccagg
    13381 caagattgtg cacccgattg gtacataacg cctgcatcag caggtgccag gcggggtgaa
    13441 aacgcacatc gtccagacgg cgtccttgcg catcgtagcg caataattca ggcggattca
    13501 cattcgccag ccgccccagt tcaagagatt cagccgttcc taactgctga ccaatactgg
    13561 ctagaaaatc gctatcccag ccagcacctt cacgcgttac cgcttcacag agcgcgccat
    13621 cagacaggta taagttgctg ttatttaatg gtataggttg attaaaaacg gtgtgagttt
    13681 gccagtgcac tgtgtctccc tccatcaatg gcagtcaccc ttagtatggt cactgccatg
    13741 gattcctgac agaaagagat tgctaaaaca ttcttatcca gcaatgtaat gcggaacgaa
    13801 acgtgaggtg tctttggtga tcagcgtgtt atcttcacgg atccccattc cgcacgcttc
    13861 atcatcgaca atccagctac cgatgagtgt gtagctatcg ccaaatcgcg gcagaggttg
    13921 aaacgcctgg tagatcatcg gttcatcggc gtaatcacca tcagcgtggt caacgacgtt
    13981 attctgaccg tcgaaaatgg tgacgtttcc gccttcgcgt gagtagatcg gtttacgcac
    14041 atagctttcg ccagcggcaa tctgcggttt ttcgccctca aaccaggatg caagaaggtt
    14101 aggatgacca ggaaagaagc gccaaagcag cggcattagc cctttattac tcaagatact
    14161 tttccataac ggctccaccc attgctcgcg acgcttgcgc agcagcggac cgttatcgtc
    14221 acgcatcatc cactccagcg gatacagctt aaatgcacgc tggatgacat tatcatcaag
    14281 atcggtcagt acgccgccga cgcccaaacc gagatcttca atgtagataa accgcgactc
    14341 ctgccctgcc tgctgggcgc agtcctgcaa gtacagcacg gtactcctgt cttcatcggt
    14401 gtcctgacag cagcaaaaat aaaacggttc ccgactgtaa agctcactaa agcgcgaaat
    14461 caggcgttcc tgaatggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc
    14521 aaaatccatc cggccataca gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc
    14581 ccagtacaac ggcgggatcg ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc
    14641 caccacctca aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat
    14701 ctggcgcaga gtaaaacggt aagcccgact ctcgtcccaa tagatttcat tgtcgatgat
    14761 atgaaagtcg aaaccgttgt tagcggcgat ctggtccaga tcccgtcgca caggaacgtt
    14821 gtgtctcagc atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg
    14881 ccgcgcgaaa tggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt
    14941 ttgccagaac cggagcgcca ggagtaatca ccggaagtgt tgagccagac agggcgggaa
    15001 gcaaatgagg atccgccgct gttatagacg aacggctcat cgcgatcttt acgaataacc
    15061 cggcttaaca aaaatccaga aacgaccggg atccaactct gttcaacatt gtcgtagtag
    15121 caattttcgt acttagactg acaattctgc tgagtcatat tcttcggaac atcggcataa
    15181 aatgccgctt tggcgttgtt ccagccacgc gcgcaaatgt cggcattatt accgtcatca
    15241 atacaatcct gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac atcgccacta
    15301 tcgccacaac ctttcaatac aaaaaaagcg gcaccgccca ttagcgccaa cgttaagtat
    15361 ttttgtgcgt agcgattgcg acgcggttca aaaggattat tcggtctacc aatgcgactg
    15421 atcgctccgt gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc
    15481 cattaccatg tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata
    15541 aacattcctg ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt
    15601 ctgacaccag caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag
    15661 tctggaatac tcaccgcatt gattgccgca ctggataagg ggatcacgta acccaacaat
    15721 gtaccgctga atgccagtga caccgcggta ttattgtttt tgattaattg ccattcgttg
    15781 tgcggtgtaa ttttggagta gataaacaga aaaataatca ccatagccac gccaataaaa
    15841 aaataggcac taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt
    15901 aaccaataat atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc
    15961 actctggctc gccgagatcg ttaaaagatt cctcaccatt taacagtaaa tattcgtaag
    16021 tatcttcggt tacttgccgc tggtagccca tggtaaaatt atggacctcc catttggcat
    16081 ggttttgatt ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc
    16141 tgttaaaaaa tcgctgccag cgtttttcct gccagtttaa ggtcattgcc cccatcgttt
    16201 tggcgttgat cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga
    16261 caaagagctt gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca
    16321 gaaattcatc gcctgaagtg tagtaacgga aaatctggct accgcctccc agatcgatgc
    16381 ggctgacagc ggctaccgta aattcctcac ccggtagcgc aatcagcaat tcatcttcca
    16441 gcaaacgaaa cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac
    16501 cgcgagcgat agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat
    16561 tttatcctta aaatagttgc cagcctttta gcgaggctgg caaaaaaatt actcgccctg
    16621 ttggcgttgc agtcttgcta atacatcctg ggctctactt ttattgctac cgccaattcc
    16681 ggcttccgcc agcttttcgt caaggtcgcg accgtctgcg actttctcca actgtgcggc
    16741 agcatccagg cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc
    16801 cgctgtcgaa acgctggaag atgcgccaac ggtagaggtt gttacagcct gttgtgcacg
    16861 ctgcatggct tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg
    16921 cgctgtcgct gtaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac
    16981 ttgctcttca gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat
    17041 caacgacgga ttaacgttct tgcttaacgc ttccagcgca cgggcttcca gactggcttt
    17101 gcgctcacgt aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac
    17161 gcgagatttt ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat
    17221 gcgcacgccc tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag
    17281 cgtaaataaa ctttttaaaa ttcccataaa tacccgtcct tgtaattagt gtgaatattc
    17341 ttctgtaatt tcagccagat ccaatgcgtt atctaccagc taggttatct caagcaggat
    17401 atcttcaaga gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcctgttg
    17461 cacgctggag attcctaccg atgacaacgg catcatcttc tgatttctgg taat
//
LOCUS       DAJTOR010000077        17477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTOR010000077 DAJTOR010000000
VERSION     DAJTOR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.077"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..625)
                     /locus_tag="OO249_002289"
     CDS             complement(<1..625)
                     /locus_tag="OO249_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5636569.1"
                     /translation="MSLNFTVIVWLRLSIGGVYELAFCRSSLKSKKYFIILLALAAIA
                     GLGTHAAWSSNGLPRIDNKTLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKG
                     TQDARELGNAFSADIPDFDLYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAI
                     KDLQSKAPDKNIVIFTHNHCLTYIAKNKRDATFKPDYLDGLVMHVEKG"
     gene            876..2015
                     /gene="arnB"
                     /locus_tag="OO249_002290"
     CDS             876..2015
                     /gene="arnB"
                     /locus_tag="OO249_002290"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5636570.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2019..2987
                     /gene="arnC"
                     /locus_tag="OO249_002291"
     CDS             2019..2987
                     /gene="arnC"
                     /locus_tag="OO249_002291"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5636571.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2987..4969
                     /gene="arnA"
                     /locus_tag="OO249_002292"
     CDS             2987..4969
                     /gene="arnA"
                     /locus_tag="OO249_002292"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR5636572.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHATRQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4966..5856
                     /locus_tag="OO249_002293"
     CDS             4966..5856
                     /locus_tag="OO249_002293"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5636573.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5856..7508
                     /gene="arnT"
                     /locus_tag="OO249_002294"
     CDS             5856..7508
                     /gene="arnT"
                     /locus_tag="OO249_002294"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5636574.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            7505..7840
                     /gene="arnE"
                     /locus_tag="OO249_002295"
     CDS             7505..7840
                     /gene="arnE"
                     /locus_tag="OO249_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5636575.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7840..8226
                     /gene="arnF"
                     /locus_tag="OO249_002296"
     CDS             7840..8226
                     /gene="arnF"
                     /locus_tag="OO249_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5636576.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8220..8486)
                     /gene="pmrD"
                     /locus_tag="OO249_002297"
     CDS             complement(8220..8486)
                     /gene="pmrD"
                     /locus_tag="OO249_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5636577.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(8596..9951)
                     /gene="menE"
                     /locus_tag="OO249_002298"
     CDS             complement(8596..9951)
                     /gene="menE"
                     /locus_tag="OO249_002298"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5636578.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9948..10910)
                     /gene="menC"
                     /locus_tag="OO249_002299"
     CDS             complement(9948..10910)
                     /gene="menC"
                     /locus_tag="OO249_002299"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5636579.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELLQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10910..11767)
                     /gene="menB"
                     /locus_tag="OO249_002300"
     CDS             complement(10910..11767)
                     /gene="menB"
                     /locus_tag="OO249_002300"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5636580.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11782..12540)
                     /gene="menH"
                     /locus_tag="OO249_002301"
     CDS             complement(11782..12540)
                     /gene="menH"
                     /locus_tag="OO249_002301"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5636581.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(12537..14207)
                     /gene="menD"
                     /locus_tag="OO249_002302"
     CDS             complement(12537..14207)
                     /gene="menD"
                     /locus_tag="OO249_002302"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5636582.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14296..15591)
                     /gene="menF"
                     /locus_tag="OO249_002303"
     CDS             complement(14296..15591)
                     /gene="menF"
                     /locus_tag="OO249_002303"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5636583.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(15670..15975)
                     /gene="elaB"
                     /locus_tag="OO249_002304"
     CDS             complement(15670..15975)
                     /gene="elaB"
                     /locus_tag="OO249_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5636584.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16030..16491)
                     /locus_tag="OO249_002305"
     CDS             complement(16030..16491)
                     /locus_tag="OO249_002305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5636585.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            16556..17473
                     /gene="rbn"
                     /locus_tag="OO249_002306"
     CDS             16556..17473
                     /gene="rbn"
                     /locus_tag="OO249_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5636586.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         4107 a         4692 c         4437 g         4241 t
ORIGIN      
        1 tgcctttttc cacatgcatg actaaaccat ccagataatc aggtttaaat gtcgcatcac
       61 gcttattttt agcaatatat gtcaggcaat gattatgggt gaaaataacg atatttttat
      121 caggcgcttt gctttgtaag tccttaattg cactataaat ctcattaccg cactgaagaa
      181 gtcgtttatc taccgtcagc tttttacccg cggaaaacca ggtcgccgac tgaatggtcc
      241 ggacggtatt actggaataa agatcgaaat cagggatgtc agcgctaaaa gcgttgccca
      301 gttcacgggc atcctgggta cctttaaccg taatacctgt tttatctgac aagcattgat
      361 tggttgaacg atcgcaacgt tcagcatggc gaaacaaaac gacaaccggg tgctgctgtg
      421 ccagtctggc caatgtttta ttgtcgatac gtggcaaacc attgctactc caggcggcat
      481 gagtacccag tccggcaatt gcagcgagcg ccagtaaaat gatgaaatat ttttttgact
      541 tcaatgatga gcggcaaaaa gctaactcgt aaactccacc tatagacaag cgcaaccaga
      601 caattaccgt gaaattgagc gacatttctg gtgataatta gcagttggtg taatattaaa
      661 aatcctacga tgtcggcaaa atgcctcaaa attttgccaa atgcaaagcc taaataagaa
      721 aaaatataaa aatttcaata tttacgtcta atattagttt cttaaggtta agttaatatt
      781 ctatccttaa aatttcgttc caaatggcaa aatatacaca acactcttta tagcaaatat
      841 aagtggacag gtattcaatg gcggaaggaa aagcaatgtc agaatttttg cctttttcgc
      901 gaccagcaat gggcgtggag gaactcgctg cagttaaaga agttctcgaa tccggttgga
      961 tcacaaccgg tccgaagaat caggcgcttg agcaagcctt ttgccagttg acgggaaatc
     1021 agcatgccat cgcggtcagt tcagccaccg ccggaatgca tatcacgcta atggcgttgg
     1081 aaattggcaa gggcgatgaa gtgattacgc cttccctgac ctgggtttca accctcaata
     1141 tgatttcctt gttgggtgca acgccggtaa tggtggatgt cgaccgcgat acgctgatgg
     1201 tcacgcctga agcgatcgag tcagccatta cgccacgcac taaagccatc attccggtgc
     1261 attatgccgg tgcgccagca gatattgacg ccattcgcgc cattggcgaa cgttacggca
     1321 tcgcagttat cgaagatgct gcccatgccg tcggtacgta ttacaaaggg cgacatattg
     1381 gcgcaaaagg taccgctatt ttttcatttc atgccattaa aaatattacc tgtgcggaag
     1441 gtggcctgat tgtaactgat aatgaaaacc ttgcccgcca gctacggatg ctgaaatttc
     1501 acggcctggg tgtcgatgcc tacgacagac aaacctgggg ccgagcaccg caggctgaag
     1561 tcttaacacc gggctataag tacaatctga ccgatattaa cgccgcaatt gccctgacac
     1621 agttagcaaa attagagcac ctcaacaccc gtcggcgcga aattgcccag caatatcagc
     1681 aagcactggc agctctcccc tttcagccat taagccttcc cgcctggccg cacgttcacg
     1741 cctggcatct gtttattatt cgtgtcgatg aacaacgttg tggtatcagt cgcgatgcgt
     1801 tgatggaagc gttaaaagaa agaggcattg gtaccgggtt acatttccgc gccgctcaca
     1861 cacaaaaata ttatcgcgag cgttttccca cgctgtcgtt accgaatacc gaatggaata
     1921 gcgaacgcat ctgttctttg ccgctgttcc cggatatgac taccgccgat gccgaccgtg
     1981 tcatcacagc ccttcagcag ctcgcaggac aataagccat gtttgaaatc caccctgtta
     2041 agaaagtctc ggtggttatt cccgtttata acgagcagga aagcttaccg gaattaatca
     2101 ggcgcaccac cacagcctgt gaatcgttgg ggaaagagta tgagatcctg ctgattgatg
     2161 acggcagtag cgataattcc gcgcatatgc tggtcgaagc ctcacaagcg gagaacagcc
     2221 atattgtgtc tattttgctt aaccgcaatt acgggcaaca ttcagcgatt atggcgggat
     2281 tcagtcacgt tactggcgac ttaattatta cccttgatgc cgatctccag aatccgccag
     2341 aagaaatccc ccgcctagtg gcaaaagccg atgaaggtta cgacgtggta gggactgtac
     2401 gccagaaccg ccaggacagc tggtttcgta aaacagcttc gaagatgatt aaccggctta
     2461 ttcagcgcac caccggcaaa gcgatgggtg actacggttg tatgctgcgc gcctatcgcc
     2521 gtcatattgt cgatgcgatg ttgcactgcc atgaacgtag cacctttatc ccgattctgg
     2581 cgaatatctt cgcccgccgt gccattgaaa ttccagtaca tcatgccgag cgtgagtttg
     2641 gtgaatccaa atacagtttt atgcgcctga ttaatttgat gtacgacctg gtgacctgcc
     2701 ttaccacaac gccgctacgt atgctgagtc tgctcggcag cattattgcg attggaggtt
     2761 ttagcattgc ggtgctgctg gtgattttac gcctgacctt cggaccacaa tgggcggcag
     2821 aaggcgtctt tatgctattt gccgtgctgt ttacttttat tggcgctcag tttatcggca
     2881 tgggattact cggtgaatat atcggcagga tctacaccga tgtccgcgcc cgcccccgct
     2941 attttgttca gcaagttatc cgtccatcca gcaaggaaaa tgaataatga aaaccgtcgt
     3001 ttttgcctac cacgatatgg gatgcctcgg tattgaagct ctgctggctg ccggttacga
     3061 aattagcgcc atttttaccc ataccgataa tcccggtgaa aaagcctttt atggttcggt
     3121 ggctcatctg gcggcggaaa gaggcattcc ggtttatgcg ccggataacg ttaatcatcc
     3181 gctgtgggtg gaacgcattg cccaactgtc accagaggtg attttctctt tttattatcg
     3241 ccatcttatt tgcgacgaga ttttgcagct cgctcccgca ggtgcattta atctgcacgg
     3301 ttcgctgtta ccaaaatatc gtggtcgcgc gccgctgaac tgggtgctgg tgaacggtga
     3361 aacggaaacg ggcgttacat tgcaccgaat ggtaaaacgt gccgatgctg gggccattgt
     3421 ggcgcaactg cgcgttgcca ttgcgccaga cgatatcgct attacgctgc atcataaatt
     3481 gtgccatgcc acgcgccagc tactggaaca gacattaccc gccattaaac acggtaatat
     3541 tctggaaatc gcccagcgcg aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga
     3601 cagtttcctt gaatggcata aaccggcatc cgtactgcac aacatggtac gtgccgttgc
     3661 cgatccgtgg ccgggtgcct tcagctatgt tggcaatcag aaattcaccg tctggtcgtc
     3721 gcgtgttcat cctcatgcca gcaaagcaca gccggggagc gtgatttcta ttgcaccgct
     3781 gttgattgcc tgtggcgatg gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat
     3841 tactatgcag ggctcgcaat tagcgcagac gctgggcctg gtgcaaggtt cacgcttgaa
     3901 tagccagcct gcctgcaccg cccgacgccg tacccgggta ctcatcctcg gggtgaatgg
     3961 ctttattggc aaccatctga cagaacgcct gctgcgcgaa gatcattatg aagtttacgg
     4021 tctggatatt ggcagcgatg cgataagccg ttttctgaat catccgcatt ttcactttgt
     4081 tgaaggcgat atcagtattc attccgaatg gattgagtat catgtcaaaa aatgtgatgt
     4141 cgtcttgccg ctggtggcga tagccacgcc gattgaatat acccgcaacc cgctgcgcgt
     4201 atttgaactc gattttgaag agaatctgcg cattatccgc tactgcgtga agtaccgtaa
     4261 gcgaatcatc ttcccgtcaa cttcagaagt ttatgggatg tgtagcgata aatacttcga
     4321 tgaggaccat tctaatttaa tcgtcggccc ggtgaataaa ccacgctgga tttattcggt
     4381 atcaaaacaa ttacttgatc gggtgatctg ggcctatggc gaaaaagagg gtttacagtt
     4441 caccctcttc cgcccgttta actggatggg accacgactg gataacctta atgcggcacg
     4501 aatcggcagc tcccgcgcta ttacgcaact cattctcaat ctggtagaag gttcaccgat
     4561 taagctgatt gatggcggaa aacaaaaacg ctgctttact gatattcgcg atggtatcga
     4621 ggcgttatac cgcattatcg aaaatgcggg aaatcgctgc gacggtgaaa ttatcaacat
     4681 tggcaatcct gagaacgaag cgagcattga ggaactgggc gagatgctgc tggcgagctt
     4741 cgaaaaacat ccgctgcgcc atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag
     4801 tagcagctac tacggcaaag gatatcagga cgtagagcat cgtaaaccga gcatccgcaa
     4861 tgcccaccgc tgcctggact gggagccgaa aattgatatg caggaaacca tcgacgaaac
     4921 gctggatttc ttcctgcgca ccgttgatct tacggataaa ccatcatgac caaagtaggc
     4981 ttacgcattg atgtcgatac ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa
     5041 atcttgagta agcataatat tcaggccagc atttttttca gcgtcggccc ggacaatatg
     5101 ggccgccatc tctggcgact ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac
     5161 gcggcatcgc tttatggctg ggatatttta ctggcaggta cggcctggcc aggtaaagag
     5221 attggtcatg ccaatgccga tatcattcgt gaagcggcta aacatcacga agtcggcctg
     5281 cacgcctggg atcaccatgc ctggcaagcc cgtagcggta actgggatcg gcaaacaatg
     5341 atcgacgata ttgcacgcgg tcttcgcact ctggaagaga ttatcggtca accggtaacc
     5401 tgttctgccg ctgcgggctg gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc
     5461 catttgcgct acaacagcga ttgtcgtggg gccatgccgt tccgtccatt gctcgaatca
     5521 ggaaaccctg gcactgcgca aattccggtg accttaccca cctgggatga agtgattggt
     5581 cgggatgtga aaacagaaga ttttaacggt tggttactca accgcatcct gcgagataaa
     5641 ggcacgccgg tttataccat tcatgcagaa gttgaaggct gcgcttatca gcataatttt
     5701 gtggatctcc tcaaacgcgc agctcaggaa ggcgtgacat tttgcccttt aagcgaactg
     5761 ttatcagaga cgttgccgct cggacaagtt gttcgcggaa atattgccgg acgtgaaggc
     5821 tggctgggtt gccaacaaat tgcgggtagt cgctgatgaa atcggtacgt taccttatcg
     5881 gcctcttcgc gtttattgcc tgctattacc tgttaccgat cagcacgcgt ctgctctggc
     5941 aacccgatga aacgcgttat gcggaaatca gtcgagaaat gctggcatcc ggcgactgga
     6001 ttgtgcccca tctgttaggg ctacgttatt tcgaaaaacc cattgccgga tactggatta
     6061 acagcattgg gcaatggcta tttggcgcga ataactttgg tgtgcgggca ggcgttatct
     6121 ttgcgaccct gttaactgcc gcgctggtga cctggtttac tctgcgctta tggcgcgata
     6181 aacgtctggc tctactcgcc acagtaattt atctctcatt gtttattgtc tatgccatcg
     6241 gcacttatgc cgtgctcgat ccgtttattg cattctggct ggtggcggga atgtgcagct
     6301 tctggctggc aatgcaggca cagacgtgga aaggcaaaag cgcaggattt ttactgctgg
     6361 gaatcacctg cggcatgggg gtgatgacca aaggttttct cgcccttgcc gtgccggtat
     6421 taagcgtgct gccatgggta gcgacgcaaa aacgctggaa agatctcttt atttacggct
     6481 ggctggcggt tatcagttgc gtactgacgg ttctcccttg gggactggcg atagcgcagc
     6541 gggagcctga cttctggcac tattttttct gggttgagca tattcaacgc tttgcactgg
     6601 atgatgccca acatagagct ccgttctggt actacgtgcc ggtcatcatt gccggtagcc
     6661 tgccgtggct gggattactc cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt
     6721 ccgcaaccgt ctatttgttg agctggacga taatgccgct gctgtttttc tccgtcgcta
     6781 aaggtaaatt gcccacctat attctttcct gctttgcatc tctggcaatg ctgatggcgc
     6841 attacgcttt gctggcagca aaaaataatc ctctggcgct gcggattaat ggctggatta
     6901 acatcgcttt tggcgtcact ggcattattg ccacatttgt ggtctccccg tggggaccaa
     6961 tgaacacgcc ggtgtggcaa accttcgaga gctataaagt cttttgtgcc tggtcgattt
     7021 tttcgctatg ggcatttttc ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt
     7081 tcgccgcgct ttgcccgctg gggctggcgt tgctggtagg attttcaatt cctgacagag
     7141 tcatggaagg aaaacatccg caattttttg tcgagatgac acaagaatca cttcaaccaa
     7201 gccgctatat tcttactgat agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac
     7261 gcgatgacat catcatgtat cgccagacag gtgagttgaa atacggcctt aattatccgg
     7321 atgcgaaagg gagatttgtc agcggtgatg agttcgcaaa ctggcttaat caacatcgtc
     7381 aggaggggat tattacactc gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg
     7441 ccattccgcc cgcagatgcc atcgatcgtc aggagcgtct ggtgctgatt cagtatcgtc
     7501 ccaaatgatc tggctaacat tagtctttgc cagcttgctt agcgttgccg ggcagttgtg
     7561 tcagaaacag gcaacctgct ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg
     7621 gctgggactg gcgctggctt gtcttggtct tgccatggtg ctctggctgc tggtcttgca
     7681 gaacgtaccg gtaggcattg cctacccgat gttaagtctg aattttgtct gggtgacgct
     7741 ggctgcagta aaactgtggc acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt
     7801 cattattggt ggcattgtga tcctcgggag tacggtgtaa tgggcctgat atggggatta
     7861 ttcagcgtca ttattgcctc agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg
     7921 ccgccgatga cgcacttatg ggattttatc gcggctctgt tagcttttgg cctggatgcc
     7981 cggatactgt tactcgggct gctgggttat ctgctttccg ttttttgttg gtataaaacg
     8041 ctacataaac ttgcgctaag caaagcctat gccttgctca gtatgagtta tgtgctggta
     8101 tggattgctt caatggtact tcccggctgg gaagggactt tttcgctgaa agcactactg
     8161 ggagtagcct gtattatgag cgggttgatg ctgattttcc tgcccacgac aaaacaacgt
     8221 tactgagttt tccctgccgc tgtacactgc cgttcccact catcggggct ataactgctt
     8281 gccgagagga ctttcaccgt atgcagcttt tcacgattaa tacaataaag cgcattctgc
     8341 aaaggcgaga gtaaatcccc cactttcacg gcgaagtcac tcttcacttc ggcgatcatt
     8401 tttatcgcgc cgccagcatc gcagagcata agtacatgtc tgttatcttg tttattgcag
     8461 cacgattttt tgaccagcca ttccattgca ttatcctgtt tgctaagagt tttcacatca
     8521 atttacagat taacagataa actctttatc aacggcaatg gaatggcaga aatgagaagt
     8581 gaaacggagg gttaattatt gctgacgttg cacccactct tttagcgcct gacgtgaaat
     8641 tttaataccg ccgtttttca gctccggcgg taaagtcagc cagcgcaccg gttgttgaaa
     8701 acgagccagc ttatctttca cccattcgcc aagatcaacg gtttgctggt catactccac
     8761 caccgctacc ggccgatgac caaactcctt gtcggcaacg ggaacgataa acacctgcaa
     8821 aaccgcagga tgtgcagcaa taacacgctc gacttcttcc ggctgaatac cctcaccgcc
     8881 actgaataac agattgtcta aacgtccgac aatggtcagc ttgccattat gcatctcacc
     8941 gcgatcgcgc gtagcgtacc agccttcgtc attaaccagt gaaaccagtt gcccgttacg
     9001 ccagtaacct tctgccatac tggcagcccg cagccacact tcattattaa cgattttcac
     9061 ttcccgaccc ggcagcggcg aaccaacgtc tgccaggccg tcggcttctt tcgcacatac
     9121 cgtggaggca aactcggtca gaccatagcc gcaaaagcaa cgaatcccct gctcgcgcgc
     9181 ctgttccgtc aactcgaccg ggatagccgc gccgccaagt aacaccgctt tcagggaaac
     9241 ggaactgcgg ttaaccagca aacgccagag ttgtgttggc accagtgaag cgtgagtaca
     9301 gcctgccagc atttgctcca atggctgttt atcacgcacc gtcatccgcg caccagcgta
     9361 taaccagcgc cacataattc cctgaccgga gacgtgaaac agcggtaaag agagcaacca
     9421 gtcatcgtga tcgccaaacg gaatcagcga taacacacct tgcgcactgg caagatgggc
     9481 ctgataagta tggacagcgg ctttcggtaa accggtggag ccggaggtca atgtcattga
     9541 acaaagacgt gttggttgcc acagagctga atgtgtgcct tcaaccatct gaatatgcag
     9601 tgaagttaac gccgggaaag tattttctcc ttccagcacc agcgcatatt gcagtgtaag
     9661 atttggcagt aattcctcaa gcaacggttg cggaagctgt ggatttacgg gcagcacccg
     9721 cgccccgcat tgcagtaacg ccagccaggc gagcagcgtt tgcggcgtat tccacgcccg
     9781 caacatcacg ccgctgccct caaccacacc ctgcgccgca aagcccgtag ctaactcatc
     9841 gacgcgagca caaagctcgc gccagttgag ttgctcgtca ttaagacgta aggcgatggc
     9901 ttctccccgc acttgccgcc agtgacgcca cggccagtca gagaagatca taacaaccgc
     9961 tccagtgcat caacttccac gacaggcagc gtgctacccg gccagcgacg tacctgctgc
    10021 gcctgcatca gatccagcgt gtccagccct ggaatggtgt ccggcgttaa ccaggcggca
    10081 atccgcgcca gttgcgttaa gcctaagctc gactcaatag aagaactgat caccgccgtc
    10141 agcctcaacg cgtgcgccgc ctgtacctgc tcgcgtactt tttccagact gcccgtgagc
    10201 gtgggtttga taactaccgc gcgcacgccc tcttcagcca caaaggcaaa atccggctcg
    10261 cgcaggcttt catcccaggc aatggcaatg ccggtttcac gggcaaacgc tcgcgaatca
    10321 tcgcgggttt tgcacggctc ttcgagaaac gcgatgcggt ggcgataatc cgggttaaca
    10381 tatttggcaa actgctgacc tttcagcggt gtccaggcgc gatttgcgtc aagacgcaaa
    10441 tgcagatccg gaattgcctc cagcaacaga ttaaccacca tgccgtcgcg cacggcttcg
    10501 tacaatccca ctttgacctt cgccactttc tcgcctggca tatcagcaag tttgaggatc
    10561 agatcgtcag gatcgccatt acacagtggt gccgcacggt aattggccgc ctgcgggagc
    10621 gtatctgcca gctccgccaa tgcacagctt acgccaaagg caacggaagg catctgcagt
    10681 agctcgcaat cgcctgccag ccagttattt acccaggcaa gcagcacgct ttgcgcctct
    10741 tcccaggttt cctggctgaa gcccggcagt ggggagatct ccccccaccc ttcgcgctcg
    10801 ccctcacgca ggcaaacgta cagcccgtcg cgggttttta accgcctgtc gcgcagaacc
    10861 acccccgcgt ccatggggat ctgccagcgg tatacctgcg cgctacgcat tacggattcc
    10921 gtttaaattt gctgaaatca ggctgacgtt tctggttgaa agcgttgcga ccttcctgac
    10981 cttcttccgt catgtagaac agcatggtgg cgttgcccgc cagctcctgc agccccgcct
    11041 gcccgtcaca gtcggcgttc agtgcagctt tcaggcagcg cagcgccatc ggactgtttt
    11101 gcagcatttc gcggcaccag cgaacggtct ctttttccag atccgccagc ggtaccacgg
    11161 tgttgacaag gcccatatcc agcgcctgtt tcgcgtcgta ctgacggcac aggaaccaga
    11221 tttcccgcgc ttttttctgt ccgacgatgc gagccatgta ggaagcgccc cagccgccgt
    11281 cgaaggaacc gactttcggg ccagtctgac cgaagatggc attatctgcc gcgatagtca
    11341 ggtcgcacat catgtgcaga acgtgaccgc cgccgatgga gtagccagcc accatcgcga
    11401 caaccggttt cggacaggta cggatctgac gctggaagtc cagcacgttc aggtgatgta
    11461 cgccggaatc atctttatag ccgccgtaat caccacgcac tttctggtca ccgccggagc
    11521 agaacgcttt atcacctgcg ccagtcagaa tgatcacgcc gacgttgtcg tcataacgcg
    11581 catctgccag tgcctggatc atctctttta ccgtcagagg acggaaggca ttgcgcacct
    11641 gcggacgatt aatggtgatt tttgcgatac catcggtgga tttttcataa cgaatgtcct
    11701 cgaaaccttc ggagcagtcg tgccattcaa ccggtgcgta aagcattgct tcatcaggat
    11761 aaatcatatt gtgtccttta gtcagaaacg caagatctgc gccagacttg cgattacgcc
    11821 agcgggattt tcccgatgcg cgttatgtcc ggcgcgagga atgacatggc agtcggcagc
    11881 cagttccgcc gccagggcgc ggaatttgct gtcacgttca ccacataaat aataaaacgc
    11941 aaatgtgcgg gcgctaaggt tagcacgtaa atcaggctgg acggcgagag aagtcgcctc
    12001 cagcatggcg gcaagggttg cgccattatt gttgctgcgc agcgccacca gctcccggcg
    12061 ttgatcgtca ttgagcgagg caaaaacagg ctgttgatac cagtcggcaa ataccgccgt
    12121 taacggttct gtgcgaaaac gctgcgccca ttggcgatcg gaacgctgac gttccgcacg
    12181 ttgttcagca ttttgcagcc ccggatgccc gccttcgaca ataaccccac aaagccccgc
    12241 cagcccctgg caagccgcca tcatcgccac ccgtccgcca agcgagtacc ccaccagcca
    12301 gaagtcaagg atgttgtaac taaccaaggt tttacgcagt aagtcggtga catcatcaaa
    12361 tccatcgacg ctaatcgccg ccgaaccacc atgacctggg agatcaacat acaaccgtga
    12421 gtagtcggca aacgcctcgc ccacttcttg ccattcgtgg caatcgccgg aaaaaccgtg
    12481 caaaaacacc agccagggta aacctggttt tccgtgtttt gcctgcgcgt gcaggatcat
    12541 aaatggctta cctgcgccag aagttgctgg agcgtttgcg caccatcggt gtcgttaacc
    12601 accatttcaa tcaccgtggt ggttggcgtg cgccaggcgt cggcaaatgc cgtttcaagt
    12661 tcctgccagt tttgcggacg atgatatttc agctcgaaca tcgcggcggc gtgctcaaaa
    12721 tggacgtttt gcggcatcag atagaaacgc tcacgctcgc tttgcggcgt tggcaacagc
    12781 gagaaaattt gcccgccgtt gttgttcacc acaattaata ccagcggcgc agaaacctga
    12841 cgcaataacg ccagcgcgtt gagatcgtaa agtgcggaga gatcgcccac aatcgccagc
    12901 gtcggtttgc cgcttgcccg ctgaacgcct gcggcggtcg aaagcagtcc gtcgataccg
    12961 ctggcccctc ggttgctgta caccgggtaa cctgccggaa gttgcgaaag cgcatcaatc
    13021 agacgtacca ccaggctgtt accgacaaac aattgcccct gttcaggcag atagtcgctg
    13081 atgcgatgcg ccagttgcgc ttcgccaaac gcatcgcgac gggcaataac cgcctgcatt
    13141 gcctgttccg ccaggcgcgg gatttcaacg caccagggct ggcgtttttc tgccggatgc
    13201 agctccagcc agtcggcaat attggcaatt aagcggcgtc cacgatggtg cgccggatca
    13261 agccgccctt cgatgtcatc cacaatccag tactcttcgg gttcacagct tgcctgccat
    13321 tgcaggagcc gtttgcccgt caggctgctt cccagttgca ccacaatttg cgcctgctgc
    13381 agctcgctgg tcgctttggc attgcctaac caaagatcgg cacacggcag cggctgcccg
    13441 gtttgtgaca gcacatcgcc aatcagcggc cagccaagag tttgcgccca cagggcaact
    13501 tttttgccct cttccgcact catgcgcccg gcaaccacca cgccgcgctt ttgtcgccag
    13561 aagaaccagt cgcgctgttt ttcactttcc agactaggcg cttcacgcag ccacggtttg
    13621 tcgtcctgcc accagtcacc cagacgctgt tgccagctaa gcccggtatc gtccatttcg
    13681 ccatacagcg gttcagcaaa cgggcagttg atatggactc cccccgcatg aagcgtaccg
    13741 agagcgtggt cgatggtaga aaccagccaa cgtgcgggga tatcctgggt cgggcgcggc
    13801 aatgaaatac tgtgcgtggg gtgagaggcg aacattcccg gctggcgaat tgcctgattc
    13861 gcgccgcagt caattagctc cggcgggcga tcggcggtta agagaatcag tttttctccg
    13921 gttaacccgg cttcaatcag tgccggatag agatttgcca ccgccgtgcc ggaggtcaca
    13981 atcaccgcca ccggctgctt gctgactttc gccagcccca gcgccagatg ccccaaccca
    14041 cgctcatcga aatgggtgtg gtgaatgaat gcggaattct ccgccgccgc taacgttaac
    14101 ggtgtagaac gcgagcctgg ggcgatacag atgtgtctga cgccgtgacg cgttaatgct
    14161 tccagaatga ccgccgccca gcgtcggtta aatgcgctta ctgacatgag tttgtccggt
    14221 atcaatattg cggctaagta taaggagcta caaaaataga ttattgatat gaatcggtaa
    14281 tgatgcgact cattactatt ccatttgtaa taaagtacgc aaccctgccg ctttgttgtc
    14341 gatttcctgc cactcttgct cggggtcgga accacggaca atgcccgcgc cagcatataa
    14401 tcgcacgaca ttgccgctaa tttttgctga gcgcagggaa acgcagaatt cgctttgttg
    14461 taatgagaga tagcccgcag aaccggcgta ccattctcgg gtgaacggtt cgtgacgggc
    14521 gataaactgt cgcgccagat cgcgcggtaa gccagcaact gctgccgttg gctgcaactg
    14581 atgtaaacag atcacatcat ccgctttgtt gagtgaagtc cagatacagc ggcgaagatg
    14641 ctgcacttta cgcagacgca gtacctgcgg cggtaaaaca tccagcgtct gggtatcggc
    14701 ctgtaatcgt tggcagatat cttccaccac cagcatgttc tcgcgctggt ttttatcatc
    14761 cgccatcagc cactcttcta actgctgcgc ctgcttatca tcaggatgat ttgctactgt
    14821 tcccgccagc gcttcagtac gcagcgcttt gtcacgccgc cgccataacc gttccggtga
    14881 agagccaaga aaagcatttt cgccatcaaa ggccatgtaa aaatggtagc aattcagatt
    14941 cagtcgacga ctggcagcca tcatcgccgc cgcgttgacc ggacttgcga aatgcaggtc
    15001 agttgcccga gcgagcacca ctttgtcgag ctcaccttcg gcgatggttt tcgttgccag
    15061 ttcgattaat tgcgtccagc ccgttttgtc cggccagtgt tgttctcgcg tggtggttaa
    15121 atgtaaccca ggcaagggct tgatactcac cagtgtggcg ataaattctt ttgcctgaat
    15181 cgcatcgtgc tgaagggagc tttcgctgaa tagcgtcagc cgcagcgtgg ctttaccgcc
    15241 acagcgtcgc cattccaggc ggggtaaaag taaattgccc tgcgacgggt caaaagcatt
    15301 cagcccccaa atgcgtaagt cggcgtgttc cgggtgctgg cgaagaaaac gttgtgcctg
    15361 gtccaacgac gtaaaacggg taatcgcgcc caggacggca gcttcttcat caccattacg
    15421 ttgttgccag tagaattgcg ggtacgtttg ctgactggcc agccagctca aggcatcaaa
    15481 agcgtctttg agagggaaag gaatatcgat aacccgaatg ccgggtgtcg ccggaatctc
    15541 ttgcgacaaa tggcgcagta gattttccag cgccgtagta agtgattgca cgcggacctc
    15601 tccctgttaa aagcctcaca ttatacgggg tactacaaaa aaaatgcagt accccggtgt
    15661 agggaggttt taacggcgtg ccagcaacag tcctagtacc agcccaacgg ccgcgcccac
    15721 accaattcct tgccaaggtt tttcgtggac gtagtcatca gcacgataaa cagcctgctt
    15781 cgcccgatag taataactgt ctgaaacctg gctaacccgt tttttcacat catccagcgc
    15841 tttttctgca cgcgctttca gctcaacata tttctgatca gcgggatcgc cagaggagcg
    15901 gagcacctct tccagtgtct cactaagcag cgtcaggtcg tcatcgatac gtgtatcacc
    15961 aaactgatta gacattctcg ttctccattt gcgtaaaacc tgtcactaac tatagacaag
    16021 ggttgttgat tacgcctgaa ttacctcgcg tgccatccca atgtgtggga taccatcttc
    16081 ttcatagacc tccgtcacag ggataaaacc gaaactctgg tagaagtttt gcagatgcgc
    16141 ctgcgccccg aggtaaacag gcttatcagg ccagtgatgc gtgcaggtgt cgagggtttt
    16201 actcatcagt tgctgcccca ctttttcgcc acgcaacgct tcactgacaa tcacccgacc
    16261 aataacgacc ggctcaagat catcatcact tttcagaatc ctcgcatatg ccaccagttc
    16321 atcatttttc caaccgagaa tatggcgatt atcccccgtc aaatcatcac catcgatatc
    16381 ctgataaggg caattctgct cgacaacaaa caccgcgcaa cgcagttgta ataaggcata
    16441 aagctgggaa accgataatt cagagtgatg cagatcttgc cattcaatca taatgctctc
    16501 cttttagatg tataccttat actagccgga atattttttg aaacgtgatg aactcatgga
    16561 attaattttt ttaggtactt cagccggtgt gccaacccgc acgcgcaatg tcacggcaat
    16621 attgcttaac ctgcaacatc cgacccagag cggactttgg ttgtttgact gcggtgaagg
    16681 cacccagcat cagctactgc ataccgcctt taaccctgga aaactggaca agatttttat
    16741 cagtcacctt catggcgatc atctttttgg tttacccggc ttgctgtgca gtcgttctat
    16801 gtcaggcatt atccaaccct taacgattta tggtccgcaa ggtatccgtg aatttgtgga
    16861 aaccgcgctg cggattagcg gctcatggac cgattatccg ctggaaattg tcgaaattgg
    16921 cgctggcgaa attctcgatg atggcctgcg caaagtaacc gcttatccgc tggaacaccc
    16981 actggaatgt tatggctatc gtattgaaga acatgataaa ccgggtgcat taaatgccca
    17041 ggcattaaaa gctgctggcg tgccgcctgg cccactgttt caggaattaa aagcgggcaa
    17101 aacaatcacg ctggaagatg gaaggcagat taacggcgca gattacttag ctgctccagt
    17161 gccaggtaaa gcgctcgcta ttttcggcga taccggcccc tgcgatgccg cacttgacct
    17221 ggctaaaggt gtcgatgtca tggtgcacga agcgacgctg gatataacca tggaagccaa
    17281 agccaatagt cgcggccata gctctacacg ccaggctgcg acactagccc gtgaggctgg
    17341 agtcggtaag ctaatcatta cccacgtcag ctcgcgctat gatgacaaag gttgtcagca
    17401 cctgttacgt gaatgcaggt caattttccc ggcgactgaa ctagcgaatg atttcaccgt
    17461 gtttaacgtt taactct
//
LOCUS       DAJTOR010000078        17284 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTOR010000078 DAJTOR010000000
VERSION     DAJTOR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.078"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..650)
                     /gene="rlmB"
                     /locus_tag="OO249_002307"
     CDS             complement(<1..650)
                     /gene="rlmB"
                     /locus_tag="OO249_002307"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5636587.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIP"
     gene            complement(831..3272)
                     /gene="rnr"
                     /locus_tag="OO249_002308"
     CDS             complement(831..3272)
                     /gene="rnr"
                     /locus_tag="OO249_002308"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5636588.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3311..3736)
                     /gene="nsrR"
                     /locus_tag="OO249_002309"
     CDS             complement(3311..3736)
                     /gene="nsrR"
                     /locus_tag="OO249_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5636589.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3941..5239)
                     /gene="purA"
                     /locus_tag="OO249_002310"
     CDS             complement(3941..5239)
                     /gene="purA"
                     /locus_tag="OO249_002310"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5636590.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5343..5540)
                     /locus_tag="OO249_002311"
     CDS             complement(5343..5540)
                     /locus_tag="OO249_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5636591.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5622..6626)
                     /gene="hflC"
                     /locus_tag="OO249_002312"
     CDS             complement(5622..6626)
                     /gene="hflC"
                     /locus_tag="OO249_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5636592.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6629..7888)
                     /gene="hflK"
                     /locus_tag="OO249_002313"
     CDS             complement(6629..7888)
                     /gene="hflK"
                     /locus_tag="OO249_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5636593.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTYPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7974..9254)
                     /gene="hflX"
                     /locus_tag="OO249_002314"
     CDS             complement(7974..9254)
                     /gene="hflX"
                     /locus_tag="OO249_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5636594.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETILADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9331..9639)
                     /gene="hfq"
                     /locus_tag="OO249_002315"
     CDS             complement(9331..9639)
                     /gene="hfq"
                     /locus_tag="OO249_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5636595.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9725..10675)
                     /gene="miaA"
                     /locus_tag="OO249_002316"
     CDS             complement(9725..10675)
                     /gene="miaA"
                     /locus_tag="OO249_002316"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5636596.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAITG"
     gene            complement(10668..12515)
                     /gene="mutL"
                     /locus_tag="OO249_002317"
     CDS             complement(10668..12515)
                     /gene="mutL"
                     /locus_tag="OO249_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5636597.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12525..13862)
                     /gene="amiB"
                     /locus_tag="OO249_002318"
     CDS             complement(12525..13862)
                     /gene="amiB"
                     /locus_tag="OO249_002318"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5636598.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13881..14342)
                     /gene="tsaE"
                     /locus_tag="OO249_002319"
     CDS             complement(13881..14342)
                     /gene="tsaE"
                     /locus_tag="OO249_002319"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5636599.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14314..15861)
                     /gene="nnr"
                     /locus_tag="OO249_002320"
     CDS             complement(14314..15861)
                     /gene="nnr"
                     /locus_tag="OO249_002320"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5636600.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHSITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15860..16999
                     /gene="queG"
                     /locus_tag="OO249_002321"
     CDS             15860..16999
                     /gene="queG"
                     /locus_tag="OO249_002321"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5636601.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQPICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGGH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16982..17035)
                     /locus_tag="OO249_002322"
     CDS             complement(16982..17035)
                     /locus_tag="OO249_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636602.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3929 a         4934 c         4333 g         4088 t
ORIGIN      
        1 gggatgctga tcaactcatc gcaatgttca cgggtcaggc gacgcatacc ttcaccttcc
       61 gcgcccatca ccagcgccag gcgtccggtc attttgctct gatagagtgt atgatccgcc
      121 tcacctgccg taccgacgat ccagatattc tcttcctgca acatacgcat ggtacgcacc
      181 aggttagtca cccgaatcag cggaacgctt tctgccgcgc cacaggccac ttttttcgcc
      241 gtggcgttga gctgggcgga gcgatctttc ggcacaatca ccgcatgaac accagcggca
      301 tctgcgctac gcaggcacgc gccgaggtta tgcggatcgg ttacaccgtc aaggatcagc
      361 aggaacggct gatcgagcga agcgatcaga tccggcaaat cattttcctg gtactgacgt
      421 cctggcttca cgcgggcgat aatgccctga tgcacggcac cgtcgctttt ctcgtcgaga
      481 tattggcggt ttgccagctg gataaccacg ccctgggctt cgagggcgtg aatcagcggt
      541 aacagacgtt tatcttcacg gccttttaaa ataaagactt cctgaaaacg ttcaggggcg
      601 cgctccagca gggcctgcac tgcgtggatg ccgtaaatca tttcgctcat taatgtactc
      661 gttgtttacg ttttgcctga tgcactacgt ttatccggcc tacatgatct ctgcaatata
      721 ttgaatttgc gtgattatgt aggccggata aggcgttcac gccgcatccg gcatgaacaa
      781 agcgcatttt gtcagcaatc taaccctctt ctgtttgaag agggtaatga tcactctgcc
      841 acttttttct tcgccgcacg cttcgctttg gtcgctgcag ctattttctg cgttttcgcc
      901 gatggctttt tcgcttttct cgcgtctttc ttcgctgctt tcggcttcgt ttttttctca
      961 ccgcggaagg cgctgtctgg ctcaaagttt acctttttac cgaccagacg acgcttgccg
     1021 ccttttttac ctgcatcgcc ttttttcgct ttctcgcgcg ccgttttacc gacgttgcgc
     1081 ggtgcgcgtt cgctggagat caggctaaag tcgattttgc gctcgtccat attaaccgct
     1141 tcgacgcgaa cttccacgcg atcgcccaga cgataagtct ggccgctgga ttcacccatc
     1201 agccgttgcc ctacctggtc aaagcgatag tagtcattgt ccagcgaaga gacatggacc
     1261 agaccatcaa tgaataagtc gtccagacgg acaaagaatc caaagccagt gacgctggaa
     1321 attacgcctt taaagacgtt acctacctgg tcgagcatga agtcacactt cagccagtca
     1381 gccacatcgc gcgttgcttc gtcggcacga cgttccgcca tcgaacagtg ctgacccagt
     1441 tgcagcattt cttccatcga ataatggtag ccgccggttt cagtggtgtt gccctgatgc
     1501 ccctgctctt tcgccagcag atatttaatg gcgcggtgca gcgtcaggtc tggataacga
     1561 cgaatcggcg aagtaaagtg cgcataggac tgcaatgcca ggccaaagtg accacggttt
     1621 tctggatcgt aaatcgcctg tttcatcgag cgcagcagca tggtttgcag catttctgca
     1681 tcaggacgat cggcaaccga ctccagcagc tccgcgtagt cacgcggttc cggcttgtta
     1741 ccgcccggca gttccagccc cagctccgcc agtactgaac ggaaagaggt aatcgcttcg
     1801 gtgctcggct tgtcgtgaat acggaacaat gctggttctt tggctttctc aacgaaacgc
     1861 gccgccgaga tattcgccag aatcatgcac tcttcaatta acttgtgtgc gtcgttacgc
     1921 tgagtctgtt cgatacgctc aatacggcat tcagcgttga aaataaactt cgcttcttcg
     1981 ctctcaaatg agatcccgcc acgttcttca cgggctttat ccagcacttt atagaggtta
     2041 tgcaactctt cgagatgctt aaccagcgga gcgtactgct cgcgcagatc ctgatcgccc
     2101 tgcagaatat gccagacttt ggtgtaggtc agacgcgcgt gagagctcat caccgcttcg
     2161 tagaatttgt agcccgtcag gcggcctttc gacgaaaccg tcatctcgca caccatacac
     2221 aggcggtcta cctgcggatt gagcgagcac agaccgttag agagcacttc cggcagcata
     2281 gggataacct gcgaagggaa gtacaccgac gtaccacgat tacgcgcttc tctatccagc
     2341 ggcgttggcg gacgcacata gtagctgacg tcggcaatcg cgacccataa acgccagccg
     2401 ccgccgcgtt ttttctcgca gtaaaccgca tcgtcaaagt cacgggcgtc ttcgccatca
     2461 atggtgacca gcggtaaatc gcgcaaatcg acacggcccg cttttgcttc ttccggcact
     2521 tcttctttca gcccggcaac ctgttgctca acagcctgcg gccagatgta cggaatttca
     2581 tgggtacgca aagcgatatc aaccgccatg ccggtgccca tattgtcgcc cagcacttcg
     2641 acgattttac ccaccgcttt ggtacggcga gtcgggcgtt gggtcagttc aacaaccacc
     2701 acaaagccca tccgcgcgcc catgatctga tcgggcggga ttaagatatc gaagctcaga
     2761 cggctatcgt caggaaccac aaagccgacg cccgcttcgg taaagtagcg accaacaatc
     2821 tggctggttt ttggcaccag tacgcggaca atacgcgctt cacgacgacc tttacggtca
     2881 gcacccagcg gctgagccag cacctgatcg ccatgaatgc aggttttcat ctgctcgctg
     2941 gagagataca aatcatcttt acgcccttca acccgcagaa agccgtagcc atcacggtgg
     3001 ccaataacgg tacctttcac caggtcgagg cgttccggca gcgcatagca ctgacgacga
     3061 gtgaagacca gttgaccatc gcgctccatc gcgcgcaggc ggcgacgcag gccttcaagc
     3121 tgctcttcgc cttcaatgtg cagttctacc gccagctcat cacggctggc cggtttttca
     3181 cgtttggtta aatgttcgag gataaattcc cgactaggga tgggattcgc gtatttttca
     3241 gcttcgcgtt cctggaaagg atcttgtgac atctcggttc ctccgttgtc atctctgatg
     3301 aagattttcg tcactccacc agcaataatt tataaagcgg ttgattctct tcaaccaaat
     3361 cggcaagcgt gtagttatcc agttccgtaa gaaaactttg cacggcctta gaaagcgcct
     3421 gtttcaaccg acaggcaggt gtaatgtggc aaaactcact gctgcaattc accagcgata
     3481 agggctccag ctcgcgcacc acatcaccaa tacgtatcgc actcgccggt ttacccaggc
     3541 gaatgccgcc attttttcca cgaacagcag tcacgtagcc ggcacgacta agttgattga
     3601 ttattttgac catatgatta cgggagacgc cgtagacgtc agtcacttca gaaatactgg
     3661 tcatccgccc ttctggcaat gacgccatgt agatcagcgc acgtaatccg taatcagtga
     3721 aactcgttaa ctgcacatca acctcaaaag ggaaatcggg aaaaaataca tttatattga
     3781 tgataaacca gccacaagct gtgtcgctaa tttatttcag ttcggggaag gaaaaaagcg
     3841 aggatttaac actgtgtcgg atagcgtaat ggcttaaccg acacagcaat gtatcacact
     3901 gttagcccgg caggcaaaat atctgccagg cgtaccagaa ttacgcgtcg aacgggtcgc
     3961 gcagaatcat agtttcagta cgatccggac cggtagagat gatatcgatc ggcacaccgg
     4021 tcagctcttc aatacgcttg atgtagttca gcgccgcctg cggcagcccg ctacgatctt
     4081 tcacgccgaa agtggattca gaccagcccg gcatggtttc gtaaatcggc tctacacctt
     4141 tccagtcgtc agctgccagc ggagtggtag tcacttcgcg accatccggc atacggtaag
     4201 ccacgcagag tttaacctct ttcaggccat ccagaacgtc cagtttagtc aggcagaagc
     4261 cagacaggga gttcagctgt accgcacgac gaacggcaac ggtgtccagc cagccggtac
     4321 gacgacgacg acccgtcgtt gcgccgaatt cgttaccctg cttgcagagg aactcgccag
     4381 tttcatcaaa cagttcggtc gggaaaggac ctgcacccac acgagtggag taagctttga
     4441 ggatacccag aacgtaatca acataacgcg ggcccaggcc ggaaccggtc gccacgccac
     4501 cagcagtggt gttggaagaa gttacgtacg gataagtacc gtggtcgata tccagcagcg
     4561 taccctgcgc accttcaaac atgacgaaat cgccacgctg acgcgcctgg tcgagcaggt
     4621 cagaaacgtc aaccaccata gaagtcagga tgtcggcaac agccatcgta tcatccagaa
     4681 ctttctggta atcaaccgct tcagctttgt agtagttaac caactggaag ttgtgatatt
     4741 ccatcacttc tttcagtttt tcagcgaagg tttctttgtc gaaaaggtcg ccaacacgca
     4801 gaccgcgacg tgccacttta tcttcataag caggcccgat accacgaccg gtggtgccga
     4861 tcgctttcgc gccacgcgct ttctcacgcg cgttatccag cgcaacgtga taatcaagga
     4921 tcagcggaca tgcttcagac agcagcagac gctcacgaac ggggatgcca cggtcttcca
     4981 gttctttcat ctctttcatc agcgcggccg gagacagcac aacaccgtta ccgatgatgc
     5041 tggttacatt ctcgcggaga atacctgatg gaataagatg gagaacggtt ttttcaccgt
     5101 tgattacgag agtatggcct gcgttgtgac cgccctggta gcgtacaaca tatttagccc
     5161 gttcagtcag aagatcgacg atcttacctt taccttcgtc accccattgg gtgcccagta
     5221 cgacgacgtt gttacccatt tttcaaaatc accgtttgct taaaaatgga ttctaccatc
     5281 gctttttcag agttacagca ctttttgcac tcaaaaatga cctcgatcag tctgtttttt
     5341 gttcagccaa tcgttttcct caacatgtag tagaccacaa cgcccgcaac cacaagtcca
     5401 ccgccaaaac gacgtaaaat attatcgggc aaattggtca tcgcagagat catcttcttc
     5461 catgccttcg ggtaaagcat cggccctaaa ccttccagta ccaaaaccag ggcaagcgcc
     5521 agccagattg tcgaattcat tcattatcct tatagaaaaa gaaaaccacc gacatccctg
     5581 aggatgcggt ggctttattg acctgtaccg cagtcgttat attaacgcgt tgcggaagtc
     5641 ggcgtcttca tgtagcggaa gaaatcgcta tccgggctca tgaccatcac gtcctgattg
     5701 ccagagaagc tgttctcata agcacgcagg ctacggatga atgcgtagaa gtccggatct
     5761 ttactgaatg catcagcaaa cagtttggct gcttcggcat cgccttcacc acgcatgatg
     5821 cggccctgac gctcagcttc tgccagcgtt ctggtcactt catagtcggc agtcgcgcgc
     5881 agtttttccg cttcttcctg accttgtgaa cggtgacgac gcgctaccgc ttcacgctcg
     5941 gcgcgcatac ggttgtagat cgcttcagac acttcggtcg gcaggttgat ctgcttgata
     6001 cgcacatcga caacttcaat acccagcgcc gccatactgt tcgggttgat gaccggaact
     6061 ttgcccttcg tctcagccgt tacgcgctct gccgcttcgg caatggcgtt atctgctgcc
     6121 ggggtagtaa cttcatcttc tgtacccgca gaaccggagt tcagcgcgtc acgtacttcg
     6181 agggtcagac gaccacggga atcagtgacg atatctttca cgtccaggcg accaatttca
     6241 gaacgcagac ggtcagagaa cttacgtttc aacagcactt ccgcttgcga aatgtcgcca
     6301 ccacccgttg ccaggtagta acggctgaaa tcgctgatgc gccatttgat gtaagagtcg
     6361 acgatcaggt ctttcttctc tttggtcaca aagcggtcgg cctggttgtc catggtctga
     6421 atacgtgcgt cgagcatttt caccgtttca atgaacggta tcttgaaatg cagacccggc
     6481 tcataaacca gaggtttgtt gtcatcgtca cgcagtacct taccaaaacg cagcgtaata
     6541 ccgcgctcac cttctttgac gacaaagaca gacatgtaaa gcactaccag cacgatgatg
     6601 ataatcgcga taactgactt acgcatcgtt attccccctg acgctggtag tcgttacgct
     6661 gcgcgttggc gcggcgttgg tccataatat cgccctgact ggtggacgac gtgttgcttg
     6721 ctccactggt tgtggaggaa gaggctggcg gcaaacgcag cagattgctg gcaccgttat
     6781 cgctcttcgc cgcaggggcg ttaccacctt tcagcatctg gtctaacggc agaaccatca
     6841 ggttgccacc tttatcgtta accagcactt tgcgggtgtt acccaacact ttttccatcg
     6901 tctcgatata cagacgctcg cgagtaattt ccggcgcggc tttatattcc ggcagaagtt
     6961 tagcaaagcg cgccacttca ccctgagctt ccaggatggt ctgggccttg tacgcacgcg
     7021 cctcttcgag gatacgttgc gcctgaccgt tcgcacgcgg ctgaacttcg ttggtatacg
     7081 cttctgcttc acgaatgtat tgctgttcgt tttcacgcgc ggcaatcgca tcgtcaaacg
     7141 ccgcttttac ttcttccggc ggacgagcag cctggaagtt gacgtccagc agagtgatgc
     7201 ccatgtcata cggacgaatc gtctcttcca gttcgcgctg ggtatcgcta cgaatcacgg
     7261 tacgaccttc cgtcagaatg cggtccatgg tgtatttacc gataaccccg cgcagggcgc
     7321 tgtcggtagc ctgacgcagg ctgtcatccg ggctggtcac gctatacaga tatttttccg
     7381 gataggtaac gcggtactgc acgttcatct caacgcgcac tacgttctcg tccgacgtca
     7441 gcatcacacc ggaagcggcc agttcacgca cggcttccac gtttaccggt ttgacttcgt
     7501 cgataaacgt cggtttccag ttcagacccg gctcaaccag atggctgaat ttaccaaagc
     7561 gtgttaccac gccgcgttcg gcttctttaa tggtatagaa accactggcc gcccagataa
     7621 tgacaatcgc tgccgctgcg atggtaacga cacgaccgcc aagctgcggg cgcgggcctt
     7681 gcgatgaact gccaccgcca gatccggtgc ctttaccgcc gcccagacca ccgagctttt
     7741 tgctcagttt gcggaagata tcatctaaat caggtggccc ttgatcgcga ccgcctttgt
     7801 ttccatttcc ctcagagttg ccgccaggtt tgctgcttcc ccacgggtcg cggtcttgtc
     7861 cgttattacc gggctgattc cacgccatgt ttgtgctcca tatttgttat gcggtgatcc
     7921 ctgttgtctt tcgacgccag aggatatgac tccacgcttc agacgctacg ccgttagatc
     7981 aggtaatcga tcaacgccgg ttcttgttta cagaggcgac gccagtcaac gatcggcata
     8041 cgaacttgca gacttacgct gccgtcctcc tccatccact ctttttctat tgcctgaagc
     8101 tgataaaaac gacttctcag acgcccttcc tgcggtggca gacgcaatgt atgctgcgcc
     8161 acctcgccgg aaagccgctc cgtcaaagcc tgaaaaagct gtggtatccc cgctccggtc
     8221 tgtgcggaaa gccagacacg gatcggtttg ttctcttcgt cccgatcaat acgcggttcg
     8281 aaatcttcca gcatatcgat cttgttcatc accagcaggg ttgggatctc gtgagcgtcg
     8341 atctcttcaa gaaccgtatt caccgcttcg atgttttctt gtacacgcac atccgccgca
     8401 tcaatgacgt gcagcagtaa tgtggcttgc cgcgtctctt gtaacgtggc tttaaatgcc
     8461 gccaccagat cgtgcggcag atggcgaata aaccctacgg tatctgcaag tatggtttca
     8521 ccgacatccg cgacgtcaat acgccgcaac gtcgggtcga gggtggcaaa caactggtct
     8581 gccgcgtaga cccgcgcttc ggtgatgcga ttgaaaaggg tagatttacc ggcgttggta
     8641 tatcccacca gcgaaacagt aggaacgtcg gctttgatac gcgattgccg cccctgctca
     8701 cgctgctttt caactctttc caggcgcgac tgtatctgca cgatgcgatt acgcaacaaa
     8761 cgacggtcgg tttcgagctg ggtttcaccc ggaccacgca aacctatccc gcctttctgt
     8821 ctttcaaggt gggtccagcc acgcaccagg cgcgtagcca gatggcgcag ctgcgccagc
     8881 tcaacctgca acttaccctc atgggtacgc gcacgttggg cgaaaatatc taaaataagg
     8941 ccggtgcggt cgatgacacg acactcgcac aaacgctcca ggttacgctc ttgcgccggg
     9001 ctcagggcat ggtcaaaaag aacgaccgaa gcacccgtcg ctttgacagc ttccgcaatt
     9061 tcaactgctt taccttcacc tacaaaatac tttgggtgcg gcgctttacg gctaccggta
     9121 atcacctgca atgcttcgac accggcggaa gaaaccagag attcaaactc ctggaggtct
     9181 tccatatctt tgtctttcgt aaaatagatg tgtaccagta ccgcctgctc accagcatca
     9241 taacggtcaa acaagcgtat aaccctctaa atagatcagc ggggaacgca ggatcgctgg
     9301 ctccccgtgt aaaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9361 ggaagtattc tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9421 gttactgtga tgagaaaccg gacgagacgg gacaacagta gaaatcgcgt gcttgtaaac
     9481 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9541 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9601 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9661 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9721 acattcagcc tgtgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9781 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9841 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
     9901 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
     9961 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10021 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10081 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10141 ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10201 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10261 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10321 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10381 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10441 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10501 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aagggcagaa tcaacgctta
    10561 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10621 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10681 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10741 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10801 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10861 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    10921 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    10981 atacccaatt ccgccagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11041 acttttagcc gcaacggaat aagcagcggc tgagcgcaaa ctggcgcttc acccggggtc
    11101 agttgtacct gacgcagcca acgttctgcc actggcaagg ctaaaagtga aatgttgccg
    11161 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11221 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttaatttttg catcggcgcg
    11281 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11341 ttcggccaag gggcagccgg acgactgcct gatgctggcg caggagtgta gcgcggagct
    11401 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11461 ggaatggaac gcggtgcagg ttggggttca tcgtccagcg gtagcggcgt ttccagttgc
    11521 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11581 aaacgcactt cgtgtttggc ggggtgcacg ttgacgtcca cctgatgcgg atcgatctcc
    11641 agatacagca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11701 atcgcgtgat tgatcaggcg atcgcgcatc atgcgaccgt tcacgtagca atactgaatt
    11761 tctgccagtg cgggcgtggt gtgatttgga tcggccaccc agccgcgtag cgtgagatcg
    11821 ccgtgttgcc attcaatcgc cagcgcttgt tcaagaaaag cggtgccgca aatcgcgcct
    11881 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    11941 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatctca
    12001 tcaatgtggt taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12061 tagaacagat ccagcacctc cagcgtcgtc cccacaggat gcgccgccgg ttttaccgtc
    12121 acgttcatat cgcgcccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12181 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12241 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12301 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12361 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12421 tccaccagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12481 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12541 cgatctggcg tcgtcaccgt actggccgtt tgtgccgttg caccctgcgg cgcagattgc
    12601 atcggatgcg caaggaaata attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12661 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12721 accagtactg acgggatatc cggcgaacgc agaacgccga gactcgcgtg ttctggtcgg
    12781 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12841 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa atagggatca
    12901 gactgactgt tcgccagcac atcgcccgcc ccacccagca gctccgaatg tttctcgtgc
    12961 tgctccagcc agctggccat ttcgctgttg gcgcgacggt tagagagcac ccatacggaa
    13021 gcgccagtcg cactgcggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattagcg
    13081 ttttgcttac gtgccacatc gctgcgcccc atcaccgaaa taaagtaatc cccgtcacgg
    13141 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13201 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13261 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13321 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13381 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13441 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13501 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccatt ttcggtaaga
    13561 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13621 agattattgc cgctgaacaa caacggcagt ccctgaatca cgcccgtttg tttgatatcg
    13681 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13741 aacgttatcc gcgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13801 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13861 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    13921 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    13981 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccaccaggca gatggcatcg
    14041 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14101 aaatcaaagt gataaaccat taaattgtcg agcgtatagg gttcgaccag cgtataagtg
    14161 gggcttttga cattaccctg atgacccaga gcttgtaaaa agccccggct aaaggtggtc
    14221 ttacctgcgc ctaaatcgcc atacagatag attacggttg cgccatcgca ggctttcgct
    14281 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14341 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14401 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14461 gacacagccc gcacaggcgg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14521 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14581 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14641 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14701 gacggaacag cctaacaacc gtgcggcctc gccaggatgc ggcgtgatca cgcgattgtg
    14761 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14821 gcgaaaattc tcaacttttt gcagtgcttt tttcccccac tcttgctggc ccagaccggg
    14881 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tcgtcagttc
    14941 atgcaccatc aattccggtc gtgcagtcag cagcggcgca atgttttcac tgcgggtcag
    15001 tactcggact aaaccagcac cagcacgcag cgccgcttcc cccgtcatac gaatagcccc
    15061 cgccgtgccg tgatcgccac cgataattac cagccgcccg tgatcgcctt tatgcgaagt
    15121 cgggcgacgc ggttttagcc agtgagaaag ttgttctgct gaaaaccgct gaattttcgt
    15181 ctcctgacct gccagccaac tatccagccc cagtgagtca aaatgcagtt gtccggtaac
    15241 atcccgcgct tttccagtga gcaagcctgg tttcagcgca ataaaagtga tggagtgatc
    15301 ggcgttgatc accgcgcctg gcgtagcgcc agtttcagcc agcaggccgg aagggatatc
    15361 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15421 gcggggcgct tgccgcaaac cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15481 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15541 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gcaaacaacg tgacctcaat
    15601 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15661 gcacagcacc agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    15721 ctcgccagcg cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg
    15781 ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt
    15841 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    15901 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    15961 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    16021 gatggcacgt cacggtatgc tgcgcgctcg ccctcatgag ttattgcccg gtacgctgcg
    16081 cgtgatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    16141 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    16201 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    16261 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    16321 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt
    16381 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    16441 taaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    16501 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcga tcccggaaga
    16561 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ctatctgccc
    16621 gtggaatcgc tattcacaac tcaccacaga agaggatttc agcccgcgta agccgctaca
    16681 cgcaccggaa ctcattgagt tattcgcctg gagcgaagag aagtttttaa aagtcacgga
    16741 aggatcggcg attcgtcgaa ttggtcacct gcgttggctg cgtaatatcg ccgtagcatt
    16801 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgggca
    16861 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    16921 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    16981 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    17041 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt aatttttaac
    17101 ttatctatca ataggttaaa aaaatactat tcaccgtgcg aagtttcttt gaaattaaga
    17161 ttacagcctg aggctgtgga taactctgtt tacaagagtt tgtcagagac aacgaaaacc
    17221 agcaataacg catcgctgtg gatattttat tgagagaaga atttggagcg ggaaacgaga
    17281 ctcg
//
LOCUS       DAJTOR010000079        17021 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTOR010000079 DAJTOR010000000
VERSION     DAJTOR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.079"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(24..2621)
                     /gene="topA"
                     /locus_tag="OO249_002323"
     CDS             complement(24..2621)
                     /gene="topA"
                     /locus_tag="OO249_002323"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5636603.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            3001..3252
                     /locus_tag="OO249_002324"
     CDS             3001..3252
                     /locus_tag="OO249_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5636604.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3288..4337)
                     /gene="sohB"
                     /locus_tag="OO249_002325"
     CDS             complement(3288..4337)
                     /gene="sohB"
                     /locus_tag="OO249_002325"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5636605.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4557..5315
                     /locus_tag="OO249_002326"
     CDS             4557..5315
                     /locus_tag="OO249_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5636606.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5312..5903
                     /gene="cobO"
                     /locus_tag="OO249_002327"
                     /pseudo
     CDS             5312..5903
                     /gene="cobO"
                     /locus_tag="OO249_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5943..6815)
                     /gene="rluB"
                     /locus_tag="OO249_002328"
     CDS             complement(5943..6815)
                     /gene="rluB"
                     /locus_tag="OO249_002328"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5636607.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(7026..8921)
                     /locus_tag="OO249_002329"
     CDS             complement(7026..8921)
                     /locus_tag="OO249_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HCR5636608.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8949..9569)
                     /locus_tag="OO249_002330"
     CDS             complement(8949..9569)
                     /locus_tag="OO249_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5636609.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9566..10447)
                     /gene="yciV"
                     /locus_tag="OO249_002331"
     CDS             complement(9566..10447)
                     /gene="yciV"
                     /locus_tag="OO249_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5636610.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRIGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10584..10628
                     /gene="trpL"
                     /locus_tag="OO249_002332"
     CDS             10584..10628
                     /gene="trpL"
                     /locus_tag="OO249_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5636611.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10605..10698
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            10720..12282
                     /gene="trpE"
                     /locus_tag="OO249_002333"
     CDS             10720..12282
                     /gene="trpE"
                     /locus_tag="OO249_002333"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5636612.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12282..13877
                     /gene="trpD"
                     /locus_tag="OO249_002334"
     CDS             12282..13877
                     /gene="trpD"
                     /locus_tag="OO249_002334"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5636613.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13878..15239
                     /gene="trpCF"
                     /locus_tag="OO249_002335"
     CDS             13878..15239
                     /gene="trpCF"
                     /locus_tag="OO249_002335"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5636614.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFNPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     GLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPTHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15251..16444
                     /gene="trpB"
                     /locus_tag="OO249_002336"
     CDS             15251..16444
                     /gene="trpB"
                     /locus_tag="OO249_002336"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5636615.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16444..>17021
                     /gene="trpA"
                     /locus_tag="OO249_002337"
     CDS             16444..>17021
                     /gene="trpA"
                     /locus_tag="OO249_002337"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5636616.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLRDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPL"
BASE COUNT         4087 a         4561 c         4256 g         4117 t
ORIGIN      
        1 taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc atcaacataa
       61 aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg ctgtttggtt
      121 ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg cgcatcggcc
      181 agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc caccagtggc
      241 gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaaaacacc cgcagcgccg
      301 tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa tggcaccgga
      361 tcttctttcg gtggtgccac ttcgccgtta cgtaaaatct tacgtgtgtt tttacactct
      421 tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat ttcagagcca
      481 catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc gccctcttcg
      541 atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg tttcggatcg
      601 atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc gcgcagcgcg
      661 ttggtttcag cgtcttcgcc ttccagtacg ttcagcactt cgttttccgg caccaggtta
      721 atggtggttt tgcaacgctc tttcggcggc agcgcataac cagaacagcc aaggaatacc
      781 ccggtgctcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc aatgctggtc
      841 agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc tttatctaac
      901 tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc ttcgtgattt
      961 gccacctggt cgaggctgtt ttccatctgt gcggtgaagt cgtagttcat taactcgcgg
     1021 aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata gaaacgacga
     1081 ttttctactc gcacgtagcc acgatcctga atggtcgaaa tgatcgacgc ataggtagac
     1141 ggacgaccga taccgcgttt ttccagctct ttaaccagcg atgcttcact gaaacgggct
     1201 ggcggcttgg taaagtgctg ggctggtgta agttcaacga gcgtcagagc atcgccttta
     1261 ttaactgctg gtaagatgcg atcttcatcg cctttacgca acgcaggcat cacttttgtc
     1321 cagccatcaa aacgcaaaat acgacctcgt gctttcaggc ggaaatcgcc cgcaccaacg
     1381 gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggctacgaa ctgacgccag
     1441 attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc cgccatcaca
     1501 ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt gctggcgtac
     1561 tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata accgcgaacc
     1621 atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt gatatagcct
     1681 gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaatcc aagacgggtg
     1741 ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact ggttgtcggt
     1801 ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac cgcagcctga
     1861 gtttgttctt tgttgaccgg acggaacggt ttgtcgttct gatgagtcac ctgtaacgcc
     1921 aacgcttcac cagatggcgt ggtcgtgctg gcatcgactt cccagaactc ttccggcacg
     1981 aacgctttaa tttcacgctc acgctcgacc accaggcgaa ccgccaccga ctgcacacga
     2041 ccggcagaca gaccacgagc gatctttttc catagcagcg gcgaaaccat ataccccacc
     2101 acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt cagctcaccc
     2161 ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac cactcggcta
     2221 tagcgcgcat catcaccccc aatcacttcc cgcaggtgcc atgcaatggc ttccccttcg
     2281 cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg tttcagttca
     2341 gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt gtgccacggg
     2401 tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt tttagccgtc
     2461 ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg caaatcgcgg
     2521 atgtgaccga cgctggattt caccacgtag tcactaccca gatacttgtt gatcgttttg
     2581 gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt acctaatttg
     2641 attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag ttaccgatat
     2701 ggactcgttc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa aattcaggtg
     2761 actgagttaa caggtcttgg cctacaggcg ctatagcacg acggaatttt ggtcgaatgt
     2821 caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa aatccgatac
     2881 gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc caccagcgtg
     2941 gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttatttaa ggagtaaata
     3001 atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc caacaaaatc
     3061 attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac gcagcgtaat
     3121 ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc aacagccaaa
     3181 agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga aaaatatcat
     3241 ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta catcaatggc
     3301 ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc cgcgctgccg
     3361 gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac ttcacggcct
     3421 tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag gcctttctct
     3481 accgcctgtt gtccgtacca gtgttcacct gttgccacct gttcaatatc cagagacgga
     3541 cgcatacgct tcacaaaatc tttaaacaac tgatgcgttt cgttcaactc ttcgcggaat
     3601 ttctcccgcc cttcttcggt attttcaccc agcaacgtca gagtacgctt atactgcccg
     3661 gcagtgtgca gttcgatatc aatatatttg cttttcagga agcggttaaa gttgggcatt
     3721 tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat tttgtccgcc
     3781 acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac agttaaagga
     3841 atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc atgcaccatg
     3901 ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc tgcgagtacc
     3961 gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc tttaaaatcc
     4021 agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc tttcgctttt
     4081 gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg tttttgctga
     4141 tgtgagccca tcagcgccgc ggccagttct tctttcatct ccttatactg ttcgctgaga
     4201 ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt gacaataatg
     4261 gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tcgccaaaaa caaaccatat
     4321 tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct cacagtttac
     4381 aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg acaaagtatc
     4441 cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca ggcataaagc
     4501 cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga gtagccatgc
     4561 attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg ggagccagcg
     4621 atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca gtgattctgt
     4681 tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa gaaactgggc
     4741 gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat tgccaacaac
     4801 tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat aatgccggat
     4861 tgctcggcga tatttgccca atgagcgaac aaaatccgca ggtctggcag gacgtcatgc
     4921 aggtcaacgt taatgccacc tttatgctca cccaggcact gcttccttta ttactcaaat
     4981 cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga cgagctaact
     5041 ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta ctggccgatg
     5101 aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc accgcaatgc
     5161 gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct gatatcatgc
     5221 cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg acctttgacg
     5281 cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc aacagcgtca
     5341 gcagcgagtg aaagaaaaag tagatgctcg tgtggcacag gccccaggat gaacgcggta
     5401 ttatcatcgt ctttaccggc aatggaaaag gcaaaaccac cgcggcattt ggtacggcaa
     5461 cacgcgcagt tggtcacgga aaaaaagtgg gcgtcgtgca gtttattaaa ggcacctggc
     5521 ctaatggcga acgcaatctg ctggaaccac atggcgttga gtttcaggtg atggcaacgg
     5581 gctttacctg ggatacacaa aaccgcgagt ctgataccgc cgcctgccgc gaagtctggc
     5641 aacatgcaaa gcggatgctt gctgattcct cactggatat ggttttgctt gatgaactga
     5701 cgtatatggt ggcgtatgac tatttgccac tggaagaagt ggtgcaggcg ttaaatgaac
     5761 gtccacatca acagacggtg attatcacgg gtcgaggctg tcatcgggat attcttgaac
     5821 tggcggatac ggtaagtgag ctgcgcccca tcaaacatgc gtttgatgcc ggtgttaagg
     5881 cgcaaatagg gattgattat taatctgtaa aaatgccgga tatgacgtcc ggcatacttt
     5941 gattaaccgt tattacgccc gccagaacga cgaccgccgc tcacctgact gtgacgtttc
     6001 accgcacgac gaatctgatt agccttcatg cgacgacggt ctttttctac agcgacttta
     6061 gagctggttt ctggcggaag ctccaccagt tcgcgcagat agttagtctg ggcgaggtcc
     6121 agctccgtcc agccaccgcg tggcagacct tttggcagtg ggatatcacc gtaacgaaca
     6181 cggatcaggc ggctcacctg cacaccaacc gcttcccaca gacgacgaac ttcacggtta
     6241 cggccttcgg tcagagtcac gttgtaccac tggttgatcc cttcgccgcc gctgaacttg
     6301 atggttttaa aagctgccgg accatcttcc agttgcacgc cacgactcaa atcacgcagt
     6361 ttcgcgtcgt caacctgacc aaatacacgc acggcgtatt cacgttcaac ttcacggctt
     6421 ggatgcatta aacggtttgc cagttcacca tcggtggtga acagcagcag accacaggtg
     6481 ttaacgtcca gacgacctac ggcaatccag cgtgcgccac gcagctttgg cagacggtca
     6541 aacaccgtcg gacgcccttc cggatcgtta cgggtacaca actcaccttc cggtttgtaa
     6601 taggccagaa cgcgacaaat ttgttcagcg gactcacgta ccgaaatcag atgaccatcg
     6661 atacgaattt tcagcccagg ggtaacttca acgcgatcgc cgagtttagc aattttgcca
     6721 tcaacactca cacgaccggc ttcaataata gattcgattt cacggcggga gccgtggcca
     6781 gcgcgggcca gcactttctg taatttttcg ctcatagagc ttccttttgg tgtcgccttc
     6841 ccaggcgtct gatatgcttt tgtgcaacag tgagtcagaa cttacatctg actcactgat
     6901 agtaaagtct gtttgaactc ttcaggcctt aagtaaacga ctcgacgttt atcggcctgg
     6961 ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga ttaacatcag
     7021 cttaattacc agccaccacc gcctccgcca cccgcgccgc cgccagcacc accaccggaa
     7081 taacttcgcc ccaacaaact ggtattggcc ccgcaactta ggtgcgtctg taatgagaat
     7141 aaaacgccgt tacgggcaat ctctggagcg cgaatcgccg aagaaagcga aggggcaatg
     7201 cgtaacgccc attccctccc caaatttgcc gatatagccc aacctagcaa cgcgtaatcc
     7261 agtttccgtg tttccccttt ccgccgacgt ccactgtgtg ttgctgcctc tttacgcgca
     7321 agatagttaa cgatagcttc accgcgggca taacgttgct gcccagtttg ggtaaacttt
     7381 ggcagaaaaa tataccccat gccagtgaga taaccggtca ggcaaacagc gctaaaataa
     7441 cccgctggca tataccagta aaagaataca tctccgacat tgatatagag agaatagaag
     7501 gccacgcctg aacaaatcag tggtaaaaac aaagtgatga ttgcacgatt aacgcaaggc
     7561 ttgtcatcat cgcgggcaaa caaaagctca agggaacaaa ataaaaaagg aataatccag
     7621 cagatcataa taaacatatc gcctaccaga ctcattcctg cccaacctac gggactcagg
     7681 ttaccgtaga gaatgatcgc caatattgcc agtccacccc attgcaaaaa cgtatcaggc
     7741 cggtaaaaca aagacttttt acgcggcaga taaaatttct gcatccgtaa aaatgccctg
     7801 cgtaagactc tgttgcgtct ccccgtaaga acgaccttat cgcctttacg aaataacgct
     7861 tccagtaata actgctctgc gggcagtaat acctcattat ttctggtcgc gccttcatta
     7921 acacaaatca ggatttgctg gttcttatca tactgatctt ccagatgaat ttttcccttc
     7981 acgatcagat ttacgatatc ggcacagaaa cctttatcgt cgtaaaccag cttcgcatcg
     8041 agacgtaaca tgccgggcgt gtaatctggc ggaatgacat cggtttcaat cacatctacc
     8101 ggcgtaaatt gcggcctgcg cttccagaga tataaccatc cacaaaccag gaatagacac
     8161 ggaaaccaaa ttaacaacga tgacgtggag ggtaaaagaa gatggctgaa aatattcgtc
     8221 gtttgtggag ccggggcatt gctaagtaac gcgtgaggcc agcggtagag taccgtgaaa
     8281 tcttcacgat aaaacggatc gcgggattct attcttccgt cctcaaggat gcggccattt
     8341 cgatatgtgt cgccctcttc tccagtaaag agatcgattt cgctaaatgg attacccgcg
     8401 atatcaggga acttgagtga aaatcgggtc ttatagattt caaacggcca gtggttacca
     8461 gtcacgttcc agattaacag atctgaatct ccctcacgca ggaaagcatt tttaacctga
     8521 taatgaattt gataatgata gtcgccttga ggcaagaaac gttgtttatc gcccacgaca
     8581 atactcatta acccgatatg ccagtcaaga tgccagggtt caggaatacc atcacgagtg
     8641 acgccaacaa tatgatagtt cagcagtgca gcgtccccat caggctgcat ccagagtcgt
     8701 ggtaaatcac gataaaatcc gtgcttaatc tgctgatttc gcgcctgaaa atgaatattt
     8761 tctttgatgt cgagcacccc atccggttgc agagtggctt caacatcaaa gagcgagatc
     8821 ccttcttcat aagaagggat ctccgggttt tctgcataac ttagagatga tacaaacagc
     8881 cctgctatca acagcaaaat gcagcgaaac ttccctgcca tagtcttcca tccgtggaac
     8941 gcaatttgtc ataagaaagg cttcacatca cccacgcctt cacgcaccac gaccggcgta
     9001 tcatcggtaa gatcaatgac cgttgtcggt ttctgtccga gatagccgcc gtggataatc
     9061 aaatccacct gtttttccag acggtcttta atttcttctg gatcggattc ggtaaattcg
     9121 ctgcctggca gcattagcga agtggaaagc atcggttcgc ccagtgcttc aagcaacgcc
     9181 tgcgcaatcg ggttagaagg cacacgcata ccgatggttt tgcgtttttc ctgcaacaga
     9241 cggcgtggca cctccttcgt ccccttcagg atgaaggtat agttgcccgg cgtgttgttt
     9301 ttcattaaac gaaacgcaac gttatccacg aatgaatagg tcgaaagttc agaaagatcg
     9361 cgacacatca gggtaaagtt gtgaccgtcc ggcagctggc gaatacgaca aatacgctcc
     9421 atcgcgtttt tatcttcaat tttacagccg agcgcatagc cggaatcagt tggataaaca
     9481 atcaccccgc ctttacgcac gatctccacc gcctggttga tcagacgttg ctgcgggtta
     9541 tcaggatgaa tataaaaaaa ctggctcata attccctctc tgtggtgttc tgcggctgtt
     9601 cccatagctg ccagacgcct tcaacgcctg cgggcagcca gagtttacga cccagttcga
     9661 tccacgggca tggctgatga aaatcagatc cttgtgatgc ccataaatga tgctgacgcg
     9721 caagtgtcgt caactgggtg cgttcattgg gcgattgctg gcactgcgcg acttccatcg
     9781 cgtcgccgtg gtgttcggca aaatgcgcaa ccaatctttt cagccattta gcactaagat
     9841 tgtaccgccc aggatgagct agcaccgcct taccgccaga atgatgaatg acatcaatag
     9901 cttgttctat tgtacaccac tgtggcggaa cgtatccggt tttcccgcgc gccagatact
     9961 ttttaaagac atctgccatt gaactggctt tgccgcactc aacaaggaaa cgcgcaaaat
    10021 gaccgcgcgt cactgcgccg ccctgcgcca gtcgttgtgc gccttccagc gccccaggta
    10081 tttgcgcttt ttcaagtctt tcggcaattt gctgcgcccg ctgattgcgc cgttctgtct
    10141 gctgcgcgag gaactcacac attagcggat gagtaatatc aatattcagc ccgacaatat
    10201 gaatttcatg attttcccag accgtggaaa tttccacgcc gggaataaga ttcagcgcca
    10261 gtcccgaacg tgaaatttcc tctcttgctg gcgcgattgc agctgtggtg tcatggtcgg
    10321 tgatcgccag ggtgccgatg cgcatctcga ctgcacggtg caccaatgct tctggcgtca
    10381 ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgcg taattcgtgt
    10441 cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg
    10501 gcaatattct gaaatcagct gttgacaatt aatcatcgaa ctagctaact agtacgcaag
    10561 ttcacgtaaa aagggtatcg acaatgaaag caattttcgt actgaaagct tggtggcgca
    10621 cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa tcagataccc agcccgccta
    10681 atgagcgggc ttttttttga acaaaattag agaataacaa tgcaaacaca aaaaccgact
    10741 ctcgaactgc taacctgcga aggcgcttat cgcgacaacc cgactgcgct ttttcaccag
    10801 ttgtgtgggg atcgtccggc aacgctgctg ctggaatccg cagatatcga cagcaaagat
    10861 gatttaaaaa gcctgctact ggtagacagt gcgctgcgca ttacagcatt aggtgacact
    10921 gtcacaattc aggcactttc cggcaacggc gaagccctgc tgacactact ggataacgcc
    10981 ctgcctgcgg gtgtggaaaa tgaacaatca ccaaactgcc gtgtgctgcg cttcccccct
    11041 gtcagtccac tgctggatga agacgctcgc ttatgctccc tttcggtttt tgacgctttc
    11101 cgtttattgc agaatctgtt gaatgtaccg aaggaagaac gagaagcaat gttcttcggc
    11161 ggcctgttct cttatgacct tgtggcggga tttgaagatt taccgcaact gtcagcggaa
    11221 aataactgcc ctgatttctg tttttatctc gctgaaacgc tgatggtgat tgaccatcag
    11281 aaaaaaaaca cccgcattca ggccagcctg tttgctccga atgaagaaga aaaacaacgt
    11341 ctcactgctc gcctgaacga tcttcgccag cagctgaccg aagccgcgcc gccgctgccg
    11401 gtggtttccg tgccgcatat gcgttgtgaa tgtaaccaga gcgatgaaga gttcggtggc
    11461 gtggtgcgtt tgttgcaaaa agcgattcgc accggagaaa ttttccaggt ggtgccgtct
    11521 cgccgtttct ctctgccctg cccgtcaccg ctggcggcct attacgtgct gaaaaagagt
    11581 aatcccagcc cgtacatgtt ttttatgcag gataatgatt tcaccctgtt tggcgcgtcg
    11641 ccggaaagtt cgctcaagta tgacgccacc agccgccaga ttgagatcta cccgattgcc
    11701 ggaacacgcc cgcgcggtcg tcgcgccgat ggttcactgg acagagacct cgacagccgc
    11761 atcgaactgg aaatgcgtac cgatcataaa gagctttctg aacatctgat gctggtggat
    11821 ctcgcccgta atgatctggc acgcatttgc acccccggca gccgctacgt cgccgatctt
    11881 accaaagttg accgttactc ttacgtgatg cacctggtct cccgcgtggt cggtgagctg
    11941 cgccacgatc tcgacgccct gcacgcttac cgcgcctgta tgaatatggg gacgttaagc
    12001 ggtgcgccga aagtacgcgc catgcagtta attgccgagg cggaaggtcg tcgccgcggc
    12061 agctacggcg gcgcggtagg ttatttcacc gcgcatggcg atctcgacac ctgcattgtg
    12121 atccgctcgg cgctggtgga aaacggtatc gccaccgtgc aagccggtgc tggcatagtc
    12181 cttgattctg ttccgcagtc ggaagccgac gaaacccgta ataaagcccg cgctgtactg
    12241 cgcgctattg ccaccgcgca tcatgcacag gagactttct gatggctgac attctgctgc
    12301 tcgataatat cgactctttt acgtacaacc tggcagatca gttgcgcagc aatgggcata
    12361 atgtggtgat ttaccgcaac catattccgg cgcagacctt aattgaacgc ctggcgacga
    12421 tgagcaatcc ggtgctgatg ctttctcctg gccccggtgt gccgagcgaa gccggttgta
    12481 tgccggaact cctcacccgc ttgcgtggca agctgcccat tattggcatt tgcctcggac
    12541 atcaggcgat tgtcgaagct tacgggggct atgtcggtca ggcgggcgaa attctccacg
    12601 gtaaagcctc cagcattgaa cacgacggtc aggcgatgtt tgccggatta acaaacccgc
    12661 tgccagtggc gcgttatcac tcgctggttg gcagtaacat tccggccggt ttaaccatca
    12721 acgcccattt taatggcatg gtgatggcag tacgtcacga tgcggatcgc gtttgtggat
    12781 tccagttcca tccggaatcc attcttacta cccagggcgc tcgcctgctg gaacaaacgc
    12841 tggcctgggc gcagcggaaa ctagagccaa ccaacacgct gcaaccgatt ctggaaaaac
    12901 tgtatcaggc gcagacgctt agccagcaag aaagccacca gctgttttca gcagtggtac
    12961 gtggcgaact gaaaccggaa caactggcgg cggcgctggt gagcatgaaa attcgcggcg
    13021 agcacccgaa cgagatcgcc ggagcagcaa ccgcgttact ggaaaacgcc gcgccgttcc
    13081 cgcgcccgga ttatctgttt gccgatatcg tcggcaccgg tggtgacggc agtaacagca
    13141 tcaatatttc caccgccagt gcgtttgtcg ccgcggcctg tgggctgaaa gtggcgaaac
    13201 acggcaaccg tagcgtctcc agtaaatctg gttcgtccga tctgctggcg gcgttcggta
    13261 ttaatcttga tatgaatgcc gataaatcgc gccaagcgct ggatgagtta ggcgtatgtt
    13321 tcctctttgc gccgaaatat cacaccggat ttcgccatgc gatgccggtt cgccagcaac
    13381 tgaaaacccg cactctgttc aacgtgctgg gaccattgat taacccggcg catccgccgc
    13441 tggcgttaat tggtgtttat agtccggaac tggtgctgcc gattgccgaa accttgcgcg
    13501 tgctggggta tcaacgcgcg gcggtagtgc acagcggcgg tatggatgaa gtttcattac
    13561 acgcgccgac aatcgttgcc gaactgcatg acggtgaaat taagagctat cagctaactg
    13621 cagaagattt tggcctgaca ccctaccacc aggagcaact ggcaggcgga acaccggaag
    13681 aaaaccgtga cattttaaca cgcttgttac aaggtaaagg cgacgccgct cacgaagcag
    13741 ccgtcgcggc gaatgtcgcc atgttaatgc gcctgcatgg ccatgaagat ctgcaagcca
    13801 atgcgcaaac cgttcttgag gtactgcgca gtggttccgc ttacgacaga gttaccgcac
    13861 tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa aatcgtcgca gacaaggcga
    13921 tttgggtaga aacccgcaaa cagcagcaac cgctggccag ttttcagaat gaggttcagc
    13981 cgagcacgcg acatttttat gatgcgctac agggtgcacg cacggcgttt attctggagt
    14041 gcaagaaagc gtcgccgtca aaaggcgtga tccgtgatga tttcaatccg gcacgcattg
    14101 ccgccattta taaacattac gcttcggcaa tttcggtgct gactgatgag aaatattttc
    14161 aggggagctt tgatttcctc cccatcgtca gccaaatcgc cccgcagccg attttatgta
    14221 aagacttcat tatcgaccct taccagatct atctggcgcg ctattaccag gccgatgcct
    14281 gcttattaat gctttcagta ctggatgacg accaatatcg ccagcttgca gccgtcgctc
    14341 acagtctgga gatggggggg ctgaccgaag tcagtaatga agaggaactg gagcgcgcca
    14401 ttgcattagg ggcaaaggtc gttggcatca acaaccgcga tctgcgtgat ttgtcgattg
    14461 atctcaaccg tacccgcgag cttgcgccga aactggggca caacgtaacg gtaatcagcg
    14521 aatccggcat caatacttac gctcaggtgc gcgagttaag ccacttcgct aacggttttc
    14581 tgattggttc ggcgttgatg gcccatgacg atttgcacgc cgccgtgcgc cgggtgttgc
    14641 tgggtgagaa taaagtatgt ggcctgacgc gtgggcaaga tgctaaagca gcttatgacg
    14701 cgggcgcgat ttacggtggg ttgatttttg tcgcgacatc accgcgttgc gtcaacgttg
    14761 aacaggcgca ggaagtgatg gctgcggcac cgttgcagta tgttggcgtg ttccgcaatc
    14821 acgatattgc cgatgtggtg gacaaagcta aggtgttatc gctggcggca gtgcaactgc
    14881 atggtaatga agatcagctg tatatcgata cgctgcgtga agctctgcca acacatgttg
    14941 ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc ccgcgagttt cagcacgttg
    15001 ataaatatgt tttagacaac ggccagggtg gaagcgggca acgttttgac tggtcactat
    15061 taaatggtca atcgcttggc aacgttctgc tggcgggggg cttaggcgca gataactgcg
    15121 tggaagcggc acaaaccggc tgcgccggac ttgattttaa ttctgctgta gagtcgcaac
    15181 cgggcatcaa agacgcacgt cttttggcct cggttttcca gacgctgcgc gcatattaag
    15241 gaaaggaaca atgacaacat tacttaaccc ctattttggt gagtttggcg gcatgtacgt
    15301 gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa gcttttgtca gcgcgcaaaa
    15361 agatcctgaa tttcaggctc agttcaacga cctgctgaaa aactatgccg ggcgtccaac
    15421 cgcgctgacc aaatgccaga acattacggc tgggacgaac accacgctgt atctgaagcg
    15481 cgaagatttg ctgcacggcg gcgcgcataa aactaaccag gtgctcggtc aggctttact
    15541 ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc agcatggcgt
    15601 ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc cgtatttata tgggtgccaa
    15661 agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg cggaagtgat
    15721 cccggtacat agcggttccg cgacgctgaa agatgcctgt aacgaggcgc tgcgcgactg
    15781 gtccggcagt tatgaaaccg cgcactatat gctgggcacc gcagctggcc cacatcctta
    15841 tccgaccatt gtgtgtgagt ttcagcggat gattggcgaa gaaacgaaag cgcagattct
    15901 ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg gttcgaatgc
    15961 catcggcatg tttgcagatt tcatcaacga aaccaacgtc ggcctgattg gtgtggagcc
    16021 gggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg gtcgcgtggg
    16081 catttatttc ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga ttgaagaatc
    16141 ttactccatc tccgccggac tggatttccc gtctgtcggc ccacaacacg cgtatcttaa
    16201 cagcactgga cgcgctgatt acgtgtctat taccgatgat gaagcccttg aagccttcaa
    16261 aacgctgtgc ctgcacgaag ggattatccc ggcgctggag tcttcccacg ccctggccca
    16321 tgcgctgaaa atgatgcgcg aaaccccgga aaaagagcag ctactggtgg ttaacctttc
    16381 cggtcgcggc gataaagaca tcttcaccgt tcacgatatt ttgaaagcac gaggggaaat
    16441 ctgatggaac gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga aggcgcattc
    16501 gttcctttcg tcacgctcag ggatccgggc attgagcagt cattgaaaat tatcgatacg
    16561 ctaattgaag ccggtgctga cgcgctggag ttaggtatcc ccttctccga cccactggcg
    16621 gatggcccga ccattcaaaa cgccactctg cgcgcctttg cggcaggtgt gactccggca
    16681 caatgttttg aaatgctggc actgattcgc cagaaacacc cgaccattcc cattggcctg
    16741 ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg agttttatgc ccagtgcgag
    16801 aaagtcggcg tcgattcggt gctggttgcc gatgtgccag ttgaagagtc cgcgcccttc
    16861 cgccaggccg cgttgcgcca taatgtcgca cctatcttca tctgcccgcc aaatgccgat
    16921 gacgacctgc tgcgccagat agcctcttac ggtcgtggtt acacctattt gctgtcacga
    16981 gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc t
//
LOCUS       DAJTOR010000080        16778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTOR010000080 DAJTOR010000000
VERSION     DAJTOR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.080"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            77..514
                     /locus_tag="OO249_002338"
     CDS             77..514
                     /locus_tag="OO249_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCR5636617.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLADILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDSYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     gene            648..1192
                     /gene="cobU"
                     /locus_tag="OO249_002339"
                     /pseudo
     CDS             648..1192
                     /gene="cobU"
                     /locus_tag="OO249_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            1189..1932
                     /gene="cobS"
                     /locus_tag="OO249_002340"
     CDS             1189..1932
                     /gene="cobS"
                     /locus_tag="OO249_002340"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5636618.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILALLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            1944..3023
                     /gene="cobT"
                     /locus_tag="OO249_002341"
     CDS             1944..3023
                     /gene="cobT"
                     /locus_tag="OO249_002341"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5636619.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGGAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            3085..4020
                     /gene="ldtA"
                     /locus_tag="OO249_002342"
     CDS             3085..4020
                     /gene="ldtA"
                     /locus_tag="OO249_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5636620.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYEFDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVPISSGSRQMF"
     gene            complement(4076..4151)
                     /locus_tag="OO249_002343"
     tRNA            complement(4076..4151)
                     /locus_tag="OO249_002343"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4116..4118),aa:Asn,seq:gtt)
     gene            4478..4849
                     /locus_tag="OO249_002344"
                     /pseudo
     CDS             4478..4849
                     /locus_tag="OO249_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4848..5282
                     /locus_tag="OO249_002345"
                     /pseudo
     CDS             4848..5282
                     /locus_tag="OO249_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
     gene            5625..6341
                     /locus_tag="OO249_002346"
     CDS             5625..6341
                     /locus_tag="OO249_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5636621.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(6797..6872)
                     /locus_tag="OO249_002347"
     tRNA            complement(6797..6872)
                     /locus_tag="OO249_002347"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6837..6839),aa:Asn,seq:gtt)
     gene            complement(6973..8616)
                     /gene="yeeO"
                     /locus_tag="OO249_002348"
     CDS             complement(6973..8616)
                     /gene="yeeO"
                     /locus_tag="OO249_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR5636622.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKVLALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWGLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            8621..8696
                     /locus_tag="OO249_002349"
     tRNA            8621..8696
                     /locus_tag="OO249_002349"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8654..8656,aa:Asn,seq:gtt)
     gene            8858..10120
                     /locus_tag="OO249_002350"
     CDS             8858..10120
                     /locus_tag="OO249_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5636623.1"
                     /translation="MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRF
                     SGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVA
                     LAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
                     ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGD
                     YQQGRQLTRLAVVLTLHLFIRSSELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRG
                     AKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMSENTVNRALRLMGYD
                     TKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAM
                     MQWWSDYLDVSREGYVAPYIYARRHKAA"
     gene            complement(10832..11716)
                     /locus_tag="OO249_002351"
     CDS             complement(10832..11716)
                     /locus_tag="OO249_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HCR5636624.1"
                     /translation="MGILEQEMKRLAQQAGGSHKTVHDRIKLVQRFCERLVLAQNVQI
                     RRVEQLKARYIEGYVRERLAQGITKRSLQNEMAAVRCILKQAGRDRLAQSERLNNRSL
                     GLSGASRNGTKRAITPEHYRYVLETARAKDPGMAAALELSRLMGLRSQEVVQSAQSLR
                     TWKQSLERGEPRLTVVFGTKGGRPRETVILDAVAIRKALDNALVVAEDRHGRLIDKPD
                     LKSAMKYWHSQASRLGLTGTYSPHSLRYAWAQDAIRHYLAQGFSEKEALAMTAMDLGH
                     GDGRGRYVAQVYGRKDTD"
     gene            12275..12421
                     /locus_tag="OO249_002352"
     CDS             12275..12421
                     /locus_tag="OO249_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636625.1"
                     /translation="MYRIKTLASVAMFCDPVFAEVCTGKRIDRPDNRRFFQVVKRWAL
                     STTA"
     gene            complement(12581..12898)
                     /locus_tag="OO249_002353"
     CDS             complement(12581..12898)
                     /locus_tag="OO249_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5636626.1"
                     /translation="MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVP
                     VTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTIDMGARSGKRLERIPDAVVNEVL
                     ARL"
     gene            complement(12899..13156)
                     /locus_tag="OO249_002354"
     CDS             complement(12899..13156)
                     /locus_tag="OO249_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR5636627.1"
                     /translation="MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLII
                     EPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIL"
     gene            complement(13505..13711)
                     /locus_tag="OO249_002355"
     CDS             complement(13505..13711)
                     /locus_tag="OO249_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003842122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5636628.1"
                     /translation="MMLQDWHPADIIAGLKKRGTSLSALSRQAGLASSTLANALTRRW
                     PKGERLIAEALGIKPEQIWPSRYR"
     gene            complement(13708..14169)
                     /locus_tag="OO249_002356"
     CDS             complement(13708..14169)
                     /locus_tag="OO249_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636629.1"
                     /translation="MATIPTQTHPLLAVPFSAATDFTVLADHCENVAETLIESEDPVL
                     RLALCGRLAACLTLLQPTLNDPIPSHLTDSLTVDALPADMPRFVPDAELLCDYSLTLA
                     QLLIGQGMQPKVEQTITGLLCELVWYFAAEMKAPRWLRTADGVKFIDEVAA"
     gene            complement(14540..16144)
                     /locus_tag="OO249_002357"
     CDS             complement(14540..16144)
                     /locus_tag="OO249_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636630.1"
                     /translation="MITKELVDESNFISQLVDIGRFDESYQQSVAFLNKLERITIRND
                     NYFIVLANIAGALVDIGQMQNNKNASELGCNLMEDNKEAFISVLGECLYYYNYGNALS
                     NLVSITNPNDHTFKSIEELVSLKNIYWRSFKLSAEEQEEFQAELSVNLANSLRSQFRL
                     SESLRYYDLTNLKGLDIPQAWVNRSMSLMVLNLISSSFSIKLLKEVRAGYIKASVSKN
                     IPPQWESFYLERIAQTNEKIAEYAVDDETDEHDEALTQQEFEVLSPYRQFCLRNHLTL
                     SEHGLYCPCVGSATDDLVIASRGGVTGDFIIPMEMVLNRLKSEFSLARHLYFDYLHPQ
                     NTDYIKYESHFLELYNDEVLGIEIEKIRTAFRLCFGILDKIAVAICELYNLYPTTKKG
                     TPQKNIYFQNFWQLDVDNRRQLFEKVKSPGLLALYSIATDLNKDKGGELAFYKEWRNG
                     LEHKFLVVHKSDKPEDIYESYQLIEDILFIKESDFIHHFEHLLQITRSAIFSFAFMVR
                     QEGMKEKKEGIHYMTRELHAINYSAD"
     gene            complement(16157..16522)
                     /locus_tag="OO249_002358"
     CDS             complement(16157..16522)
                     /locus_tag="OO249_002358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636631.1"
                     /translation="MVSDKRADIIVSLPQIKIPIEIKRDYHRDVWTALNGQLDKLYTK
                     NPDAAGHGIYLVFWFGSARPNSLPHLAKNTSQPENASAMENMLNETVPVDKRDRLSAI
                     VIDVSCEGIPPVEAPKSSV"
BASE COUNT         4424 a         3961 c         4029 g         4364 t
ORIGIN      
        1 tcaaggtgtg gcaatattgt ttcatggctt atcaaccaga gcgccattac aggaaattaa
       61 gtggagggcg agaggcatga aaacgataaa acccgaatcg atagctcttt tttgcctctt
      121 atctggagat gtggatctgg cgaaacatct ggctgatata ttgccaataa cctgttttgt
      181 tggtgaggac tggcagcaag atttccagcc tcttgatggt gggcctgtgc aatcgttatc
      241 agattcgtat agcgattatt cagtgctgct attgatagca gctgaaagcg agatatccgt
      301 accagaatgg ctcgctcatt ctgagggaac gacggtaatt ttgattgcag aacacaatgc
      361 ccgtttgctg actggtccgg ctggtggggc tgtagcatta atccattatc tgaatgaaat
      421 ctgtcattcc agagaaaccg ctgtcaatga cgcattttgt cggacgttaa cacgaggaag
      481 tctggctatt ccgcaatgcc atcttctttc gtaatggatt gagaatgctg gatttttaat
      541 tatattacgc gcagtattaa tcgcgtaaat gttttgcggt cagcaacggt gggggatcca
      601 ttgttctgtt caatgaatac catcatacaa ttgaatcagg agtcggtatg atgatttcgg
      661 tgacgggggg catacggagc gggaagagtc gccacgcaga ggcgcttatt ggggactctt
      721 cacaggttct gtatacgcca cctcgcaaat ccttgatgat gagatggctg cacggataga
      781 acatcatcgg caaggccgcc cgacgcactg gcacacagtg gagcgctggc aacatcttga
      841 tgaattaatt catgcagaca ttaacccgca tgaggctgta ttgctggaat gcgttaccac
      901 catggtgact aatctgttgt ttgattatgg cggcgataaa gaccctgatg aatgggatta
      961 tcaggcgatg gaacaggcga ttaatgctga gattcagtcg ttgattgctg cctgccaacg
     1021 ttgtcccgca aaggttgtat tagtgactaa cgaagtggga atggggattg tgccggagag
     1081 tcgtctggca cgacattttc gtgatattgc cgggcgggta aatcagcagc tggccgctgc
     1141 ggcaaatgaa gtatggttgg tggtttcggg tattggagta aaaatcaaat gagtaaatta
     1201 ttttgggcga tgctctcttt tattacgcgt ctgcctgtac cgcgtcgctg gtcccaggga
     1261 ctggatttcg agcattattc tcgcggtatt attacttttc ctttaattgg attattgctt
     1321 ggcgcgatta gcgggctggt cttcatggtg ctgcaggcat ggtgtggcgt accactggcg
     1381 gcactgttta gcgtacttgt gctggcgctg atgaccgggg gattccacct ggacggcctc
     1441 gccgatacct gtgatggcgt attttctgca cgtagccgcg atcgcatgtt ggaaatcatg
     1501 cgtgatagcc gtttaggcac ccacggcggt ctggcattga tttttgttgt actggcaaag
     1561 attctggtat taagcgagtt ggctctgcgt ggcgaaccga tccttgcatt gctggcggca
     1621 gcatgtgcgg tcagccgcgg gactgccgca ttattgatgt atcgtcatcg ttatgcccgg
     1681 gaagaaggac ttggcaatgt gttcattggc aaaattgatg ggcgacaaac ctgcgtcaca
     1741 ctcggcttag ccgctatttt tgctgccgta ttattgccgg gtatgcatgg tgtggctgct
     1801 atggtggtga cgatggtggc gattttcatc ctcggtcagc ttctcaaacg tacgctgggc
     1861 gggcaaaccg gcgatacgct gggagctgct atagagcttg gtgaactggt cttcctgctg
     1921 gcactgctat aaagagacgt gacattcaaa cacttgccga tttactgaat acgatccctg
     1981 ctatcgatcc tgccgctatg tcgcgtgcac aacggcatat cgacgggtta ctcaaacctg
     2041 ttggtagcct gggaaggctg gaggcgcttg ccatacaact ggcaggaatg ccggggttga
     2101 atggcgttcc gcatgtgggc aaaaaagcgg tactggtcat gtgtgccgat cacggcgtct
     2161 gggaggaagg gggcgctatt tccccaaaag aagtgacagc cattcaggct gaaaatatga
     2221 cccgcggaac aaccggcgtg tgtgtgctgg cagcacaagc gggcgctaac gtctacgtaa
     2281 ttgatgttgg tattgatact gctgagccta tccccgggct tatcaacatg cgtgttgctc
     2341 gaggtagtgg caatattgct tcagctccgg caatgagtcg ccatcaggca gaaaagttgc
     2401 ttttggacgt catatgttat acgcgggagc tggcaaaaaa cggtgttacg ctgtttggtg
     2461 taggtgaact ggggatggca aacacgacac cggcagcggc aatagtcagc acaatcactg
     2521 gccgggctcc tgaagaagtg gttgggattg gcgcaaacct gccgacagat aaactggcta
     2581 ataaaattga tgttgtgcgc cgggcgatta cgttgaatca accaaatccg caggatggcg
     2641 ttgatgtcct ggcaaaagtg ggtggatttg atttggtcgg aatggctggc gtgatgttag
     2701 gcgctgcgtc ctgcggttta cccgtgttgc tggatggatt tctttcttat gctgctgcgc
     2761 tcgcagcctg tcagatgtct cctgcaatca aaccgtatct cattccttct cacctgtcgg
     2821 cagaaaaagg agcgcgtata gcgctgtcgc atttggggct ggagccttat ctcaatatgg
     2881 agatgcgttt aggtgagggg agtggcgcag ctctggcgat gcccatcatc gaagctgcct
     2941 gtgcgatata taacaacatg ggcgaacttg ctgccagtaa tattgttcta ccggggaata
     3001 cgacttctga tttgaacagt taaaaacgaa aagtctgata aaagctatac ttaacggata
     3061 gctttcgcga cttaggaaag ggacatgatg cgtcgtgtaa atattttttg ctcatttgct
     3121 ctgctttttg ccagccatac tagcctggcg gtaacttacc cattacctcc agagggtagc
     3181 cgtttagtgg ggcagtcgta tactgtaact gttcctgatc acaataccca gccgctggag
     3241 acttttgccg cacaaaacgg gcaagggtta agtaacatgc tggaagcgaa cccgggtgct
     3301 gatgttttct tgccgaagtc cggctcgcaa ctcaccattc cgcagcaact gattttgccc
     3361 gccactgttc gtaaagggat tgtggttaac gtcgctgaga tgcgtcttta ttactaccca
     3421 ccagacagta atactgtgga agtctttcct attggtatcg gccaggctgg gcgagaaacc
     3481 ccgcgtaact gggtgactac cgttgaacgt aaacaagaag ctccaacctg gacgccaacg
     3541 ccgaacactc ggcgcgaata tgcgaaacga ggggagagtt tgcccgcatt tgttcctgcg
     3601 ggccccgata atcccatggg gctgtacgcg atttatattg gcaggttgta tgccatccat
     3661 ggtaccaatg ccaattttgg tattgggcta cgggtaagtc agggctgtat tcgtctgcgc
     3721 aatgacgata tcaaatatct gtttgataat gttcctgttg ggacgcgtgt gcaaattatc
     3781 gaccagccag taaaatacac cactgaacca gatggctcga actggctgga agttcatgag
     3841 ccgttgtcgc gcaatcgtgc agaatatgag tttgaccgaa aagtgccatt gccggtaacc
     3901 ccatctttgc gggcgtttat cagcgggcaa gaagttgatg taaatcgcgc aaatgctgcg
     3961 ttgcaacgtc gatcgggaat gcctgtgcca attagttctg gttcaagaca gatgttttaa
     4021 gagcgttggt aattagaaag caaaaagcct gctagaaagc aggctttttt gaatttggct
     4081 cctctgactg gactcgaacc agtgacatac ggattaacag tccgccgttc taccgactga
     4141 actacagagg aatcgtgtga acggggcgca tattaatggc agtgcatggt gatgtcaaag
     4201 ggtgaaatgc atttttttgt ttgtttgctg tcaatttcaa caaagtggtg agctttcaat
     4261 cttattgggt aatgaaccat cgtggtgcat accctccttt tatagggcag gggaacgcga
     4321 cagctgatta aaggagcaaa tgacgaatct atcataatct cttatttgac agttggttag
     4381 cttgtacatc aacgccaaaa taaaactggc aagcatcttg caatctggtt gtaagtaatg
     4441 gcggcacttg ggccgattct taaaaaccgg aggcaacatg aacttcagac gcctgaaata
     4501 cttcgtaaaa attgtagata ttggtagcct gacccaggct gctgaagtat tgcatatcgc
     4561 acaaccagcg ctcagccagc aggttgccac actggaaggt gagttaaatc aacaactttt
     4621 gatccgtaca aagcggggcg ttacgccaac agacgccgga aaaattctct atacccatgc
     4681 gcgggccatt ttacgtcagt gtgaacaggc ccaactggcg gtgcataacg ttggtcaggc
     4741 attatcgggg caagtctcga ttggctttgc accaggaacc gctgcgtcat ccatcaccat
     4801 gcccttatta caggcggttc gcgctgaatt tccggagatc gttatctatc ccgagcattg
     4861 ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt gagcggcagg cccggtgagg
     4921 aagtcaaaca gcggctacgt actggtactg tggtaaccac agatgacagg aactgggaat
     4981 tacgttactc agcttctgca cttcgtttta acttaagccg ggccgtagca attgatatgg
     5041 aaagtgcaac cattgccgcg caaggatatc gtttccgcgt gccatacggg acactactgt
     5101 gtgtttcaga taaaccgttg catggcgaga ttaaacttcc cggccaggct aaccgttttt
     5161 atgaaggcgc tatttccgaa catctgcaaa ttggcattcg ggcgatcgat ttgctgcgcg
     5221 cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt taatgagccg ccgttccgat
     5281 aataagttct gaaccttctt atcagaaata gatgtgaagg agtaagtaag acacctggca
     5341 aatagcctgc aataatcgtg ggctgtttgc ttccttgggc ggatacgtgt tttattatcg
     5401 tcttaatgat ttccacatat taaaagcaag tatgctttca aaacacaatt ataaaaaatc
     5461 ccgccaacaa tataagtttt tataaaatta aatataagat tatggcttta gaatattttt
     5521 atttctaata gacgagattt ttcctgttat gatataatat gccgaattaa cacatgttaa
     5581 cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga cgtaaatggg
     5641 ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt tatgcaaaat
     5701 tcggtgtaga aatctatgct gctgctaaac aaggtgaacc cgatccagaa ttaaacacat
     5761 ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac gttattgata
     5821 aagcaattga taaagccaaa ggcggcggag atgaaacgtt cgtgcaggga cgttatgaag
     5881 gctttggtcc taatggctca atgattatcg ccgagacatt gacttcaaat gttaaccgta
     5941 cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga gcggcaggtt
     6001 ctgtcagcta tatgtttgac aatactggtg tgattgtatt taaaggatca gaccctgacc
     6061 atatttttga aattttactt gaagctgaag ttgatgttcg tgatgtgact gaagaagaag
     6121 gtaacattgt tatttatacc gaacctactg accttcataa aggaatcgcg gctctaaaag
     6181 cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa tctgaagttg
     6241 agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt gaagatgacg
     6301 acgatgtaca aaaagtttat cataacgtcg caaatctcta attatatatt aaagaaatct
     6361 gtctttatgg cagatttctt taatctcata taatccttat aaaaaatata atgttcaact
     6421 cgtcatattg cttataaaaa atcctcgttc ccagagaaat aatatttatt ataattccag
     6481 ttcttctttt tctgattaca gaaggcaaag tgacaattac gcatagtttc ccgataaaga
     6541 cgcgacagct acatcccgca taaggcattt ttctctttat ccttgtacgg tacttcatgg
     6601 aacagagttt ttgaccttgc gaatcgtgat gtctgttggg gaggggcaat ttgctcactg
     6661 aagcgtgaga ctcgattaag cgcacgaaac acagaaatca aaaaacccgg gcactttttt
     6721 acaaggtaac cgggtaaaaa taatttttat tttttaactg ttttgagact catagagatg
     6781 tctcaaaact aaaatttggc tcctctgact ggacttgaac cagtgacata cggattaaca
     6841 gtccgccgtt ctaccgactg aactacagag gaatcgtgag aacgaggcga atattagcga
     6901 tgcccaccca caatgtcaaa gcctgttatt taaatttgaa atcgtttgct gaaataatct
     6961 gcattttgtc gtttattccg acacaactgg ctttttttca cacttttgcg gttcggatcg
     7021 agggtatttc cacagccaac gtccagtaac cattcgccag taaaacagca cggcccgcac
     7081 agcccagtcg gcaaacattc ccatccagac accaaccaca ccccagccaa gcatgatgcc
     7141 cagcacataa ccgacgacaa cccggcaacc ccacatgctc aacatagaaa cccacatggc
     7201 gtaacgggca tcgcgagcac ctttaaatcc agcgggtagc ccccatgagg cggaccaaat
     7261 aggcataaat aaagcattta gccaaatcag aatcacaacg acatgtttaa cctgtggatc
     7321 ctgggtgtaa aacgatgcca taaccccggc aaagggagcc gttagccagg cgatggccgt
     7381 taatccaaga gtggaaagcc agaacacatg ccgcaactga atctctgctt gcgctatctg
     7441 ccctaccccc aaccttcggc ctgtaatgat cgtagaagca gagccgagcg cacttccggg
     7501 taagttgata agagccgcaa ttgaaaacgc gataaaattt ccggcaataa cactggtccc
     7561 catcccggca acgaacattt gggttaataa ccgaccactg gtaaataaca ctgattcgac
     7621 actcgcggga ataccaatcc ccatgacttc ccagataatg ctaaaattca gcggtttaaa
     7681 atagctcttt aacgaaatcc ttagcgcagg attaaaacca atcgccagca cccacaaaat
     7741 tgcaactgcg ccaatataac gagaaatggt taaacccagc cctgccccga caaatcccag
     7801 tcccggccag gagaaaaggc cgtaaatcaa tatgccgcta ataataatat taagaatatt
     7861 caggctaccg ttaatcaata gcggtatttt cgtattccct gcaccacgaa gtgccccgct
     7921 accaataaga gtgatggcag ctgctggata actgagtacc gtcagctcca gataagtcaa
     7981 cgccagtact ttaacttctg tcgtggcatc acccgcgacg aaatcaataa tttgttcgcc
     8041 aaaatgatga ataagcgttg ccaacagtac ggcaaacaac gtcatgatca ccaatgactg
     8101 ccgcgtcgcc accctcgctc gtcgtcgatc ccgcttaccg agactaaatg ccacaacgac
     8161 agtagtacca agatcgatag cagcaaaaaa agccataatg accatattga agctgtccgc
     8221 caatcccacg ccggccatcg catcttttcc cagccagctg accagaaaag tgctcagaac
     8281 ccccatcaac aggacacagg cattctccat gaagatagga acagcaagcg gggttatctc
     8341 gcgccagaac aacactttgt agctcttgcg tttagcgtgc cagcgagtgc cgtgaacaac
     8401 ctggcgtaaa gcagaggaga tattcaaagc cgaccttaat tgcagaaagt gaaaccacat
     8461 ttcaaataat gagggagaat cagcaaagct gcaaagattt tcgccaacaa attgtctgca
     8521 aatgcaacaa actgttgata gaaacggcaa acagttgggg aatttaaaaa tcgggtttga
     8581 caaaagattt ttcgccgtta agatgtgcct caacagcgat tcctctgtag ttcagtcggt
     8641 agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt
     8701 ccgattctta cgctatctta ttagtcctta tttgatattg attcaataag ttagcgtgaa
     8761 aatctttcca gatgtgtatt cagttttccc tccgcatccg gttaatttgg tggtcagatt
     8821 tgggggcagg ttggttcgat tctggagtga cccccaaatg tctttaaacg attcaaaaat
     8881 ccgtaactta aaaccctctt caaaacccgt aaaactgtct gattcgcacg gtctgtatct
     8941 gctggtcaat cagggcggtt cgcgtatctg gtatctcaag tatcgcttca gtggtaaaga
     9001 gtcccgcgtc agtctgggcg catatcctct ggtttcactt gctgaagcaa gacaacgacg
     9061 tgacgatatc cgtaagctac tgacgcagaa tattaacccc gcccaccagc gcatgtctga
     9121 taaagccgca gcgtcaccgg aaaagtattt taaagccgtg gcgcttgcat ggcacaaaac
     9181 caacaaaaaa tggtcgcctg attatgccgc gcgcatcctc gccagcatgg aaaatcatat
     9241 ctttccggcg attggtcatc tgcctgtcac tacgctgaaa acgcagaatt tcactgcgtt
     9301 actgcgggtt attgaggata aaggttttct ggaagtcgcg tcccgaaccc ggcagcaact
     9361 ctgcaacatc atgcgttacg ccgtacagca gggattcacc gaaaacaacc tggcgcagca
     9421 tctggaaggc gtcaccgcgc cgccggttaa gaatcactat cccgccctgc cgctggagcg
     9481 gttgcccgaa ttgtttgagc gcattggcga ctatcagcag ggacggcagc ttacgaggct
     9541 ggcggtggtg ttgactctcc acctgtttat ccgttccagc gagctgcgct ttgcccgctg
     9601 gggtgagata gatttcagaa acaaaatctg gaccatcccc gccacaaggg aagcgattga
     9661 gaaagtccgt ttctctggcc gtggcgcaaa aatgcgtact ccatatatag tgccgctttc
     9721 tcgtcaggct atcgccattc tgaagcagat aaaggaaatc tccggccatc tggaactggt
     9781 gttccccggc gaccataacc cgtataagcc gatgagcgaa aacactgtca accgggcgtt
     9841 gcgtcttatg ggctatgata ccaaaactga tgtctgtggg catggattcc ggacaatggc
     9901 gtgtagcgca ttagtggaaa ctgaactctg gtcacgggat accgtggagc ggcagatgag
     9961 tcatcaggag cgcaacagcg ttcgggcagc gtatatccac cgggctgaac atctcgacgc
    10021 ccgtacagcc atgatgcagt ggtggtcgga ttatctggat gtaagtcgcg aggggtacgt
    10081 tgcgccgtat atttatgcgc ggcggcataa ggcggcctga gacagtggct atataatttg
    10141 ttaaaaatga aaaagcagat cctgattgaa tccggatctg ctttttacat taggcaatcg
    10201 ctgccagaca atctgcacca gaaacgccat aactgcacca gttcagtacc accgcttacc
    10261 tgcgccagtc tgccgtcgcc tgtctgtgtc aggactgcgc cagatcttat cgaataaaca
    10321 tcaaactgcg ccagtgttac ggccctttca gccctttggc cttttccggt ctggcgctgt
    10381 tacccttagc cgccccttac cctttccggc ctttttcctt tgctggcgca atcgccagaa
    10441 aaatcgtcaa gctgcaccag aagcctgcca ataggtcata ccgttcagaa gcatcgttat
    10501 tgagaggaaa ccgtacaaaa aatttctacg tagaaatttt ttcggtggat aacgatgaat
    10561 aaagagtatt ttataatgtg atggctatca aaaatcgacc atccaaaaat aattcattga
    10621 tttataatga ataaccatga atttaatagt cgatgattat tgtttttatc agtattgata
    10681 ggaaagtata caaaaaattt tggccccaaa attttttgta cgaaaaccga tgaataacgc
    10741 gaaattaata ggaaaccatc caaaattatt tggggcaaat aattttgccc gaaaaccgat
    10801 ggataactcg atcttttcaa agcaacggct atcaatcagt atcctttctt ccgtaaacct
    10861 gcgccacata ccgcccgcgc ccgtcgccgt gtcccaaatc catcgccgtc attgccagcg
    10921 cttctttctc actgaatccc tgcgccagat aatgacgaat ggcatcctgc gcccacgcat
    10981 agcgcaacga gtgcggggaa tatgtgccag ttaacccgag acgtgacgcc tggctgtgcc
    11041 agtatttcat cgcgcttttc aggtcgggtt tatctatcag cctgccgtgg cgatcttctg
    11101 ccacaaccaa cgcattatcc agcgcttttc tgatagcgac agcatccaga ataacggttt
    11161 ctcgcggacg cccgcctttg gtgccgaaca ccacggttaa acggggctca ccccgctcca
    11221 gcgattgctt ccacgttctt aatgactgcg cactttgcac cacctcctgc gaacgtaatc
    11281 ccatcagcct tgagagttcc agcgccgctg ccatccccgg atcttttgcg cgggcggttt
    11341 ccagcacata gcggtaatgc tccggcgtga tggcccgctt cgtaccgtta cgggatgcgc
    11401 cagacaggcc cagcgagcgg ttattcagtc gctcactctg cgccagcctg tcacgcccgg
    11461 cctgtttcag aatgcagcgc accgccgcca tctcattttg cagggaacgc ttcgtgatac
    11521 cctgcgccag tcgttcacga acatatccct caatataccg cgccttaagc tgctccaccc
    11581 ggcggatctg cacattctgt gccagaacca gacgctcaca aaaccgctgc accagcttaa
    11641 tacggtcgtg aacggtctta tggctgccgc ccgcctgttg tgccaatcgc ttcatttcct
    11701 gctccagaat ccccatacat tccccctttc atgattgccg caaactcatc cccaaaaact
    11761 caacactgat acagcgttcc ccggcgcaca ggccgatagc ccctgagttt tgtcggggct
    11821 gcgaacgata cctgagcgcc ggggcggcag ccgttgaggt gtggtggggc ttcggttgtg
    11881 ctctcagagc agccgcccgc ttgcgcgggt catcccccgg atcgtccaga tccgcctcgg
    11941 tatcggttca tattctcctt atgggtaaat ggcctaaaaa ggcggtgtca gaatccagcc
    12001 atgagctggc gcagttgagt tttctggtgc aggtcgaaag ggcttgcgtg cgtcactttc
    12061 ccgctttgcg cggttaaagt gacgcacgga tctggtgcag catctgagat ttatcgtctg
    12121 gtgcagtttt acggtcacgc ggcagcaaag cctgacggca atgcaggccg cagcccgtaa
    12181 ggtaaaaggc gctgagggtg tcagcagatg caggttagcc gggagaattc cggcgcggtg
    12241 cagattcaga ccagcaggct ggcgaagtaa aaggatgtat cgtattaaaa ctctggcctc
    12301 cgtcgcgatg ttctgcgacc cggtgtttgc agaggtctgc accggtaaac ggatcgaccg
    12361 tcctgataac aggcgcttct ttcaggttgt gaagcgttgg gcgctgtcta cgacagcata
    12421 gagcaagggg gagactacgc ctgtgccgct ggcgcggaaa ggagaaactg tttttacctg
    12481 caaaaggatt ttttaatcac ttgttgttat aaaaaatgag ggcgctgtat gtgacagcgc
    12541 cctcgttcag gttacataaa aaatatcagc tcagaatagc ttacagtcga gccagcacct
    12601 cattcacgac agcatcgggt atacgttcca gccgcttacc gctccgagct cccatatcaa
    12661 tggttctggg ctggtcgcag cgaataacgc ccgttgtttt tgttcccgcc ccgtccagtg
    12721 aaacggtaaa accggcttcg cgggcaaagt ttccgccgct ggtgacaggg acaacaacag
    12781 gcaaccgggt gaacgtgtta aacgatgcct tcgatacgat gagtaccgga cgttttccac
    12841 tttgttcatg gccagtggtg ggatccagtg aaacgagcca gatttctcct ctgtccatct
    12901 acaggatctc cttgcccacc gcaggcgcat caatccattc ccgaccctct tcgcttattt
    12961 cagcgtgcgg atcgcactgc gccaacagtt cttcgagcga atagcgggga cgtttttgag
    13021 gttcaataat caggcagcca ttatcaatgg tcatgcccac ttcgctgtcc gtcgacagcc
    13081 ctagcgtttt cagcactgcg gggggaaccg ccagcatgac ggatccgcca acctttttca
    13141 ggcgagtggt atacatagag cacctccgga tattatattt taatataaca tccatgatag
    13201 acgatgcaca actattcatc agattcattg tggtttcctt agtgtaataa agcgtgaaga
    13261 aagccttaat ccatcggaag acagatccgg cagttttcaa aggcgacatt gccggtaaaa
    13321 gggcaaccgt cctgatacat caggcgcttc tttcaggtca tgaagcattt cgtacaggcg
    13381 ggagcttagt gcgtaacaaa gaaggccgat aataggaaac tgcttttgca gttcaaagga
    13441 ttatgacggt tcggataaac aaaatgcggc gcagcttccg tatccgggct gcgccgcagg
    13501 aacatcagcg gtagcgcgaa ggccagattt gctcaggctt gatgcctaac gcttcggcaa
    13561 ttaacctttc gcctttgggc cagcggcggg taagcgcatt agccagcgtg gaggacgcca
    13621 gacctgcctg acgcgaaagc gctgacagcg aagtgcctcg ttttttcagc ccggcgataa
    13681 tgtcggcagg atgccagtcc tgcagcatca tgcagccacc tcgtcgataa acttcacgcc
    13741 atcggcggtg cggagccagc gcggggcttt catttcggcg gcaaaatacc agaccagttc
    13801 acataataag cctgttatcg tctgctccac ttttggctgc atcccctgcc cgataagaag
    13861 ttgcgccagc gtcagtgagt aatcacaaag taattctgcg tcggggacaa atcggggcat
    13921 atcggcaggt agagcatcaa cggtcaggct gtcagtcaga tgagacggga tggggtcgtt
    13981 cagcgtgggt tgcagcaaag tgagacaggc ggcaagtcgc ccgcaaagcg ccagccgcag
    14041 caccggatct tcgctttcga tcagggtttc ggcgacgttt tcgcagtggt ctgccagtac
    14101 ggtaaaatct gtggcagcac tgaagggaac agcaagtaac gggtgtgtct gagtggggat
    14161 cgtagccata aaggcagcct ccagtatgtg gtttgaaaac caccaccaga ggttccaatc
    14221 tcatgggtgg tggactgaac gaggttggaa ctaccggcca tactggaacc ggcgcacctt
    14281 acggtgcccc cgcccagccc accataattt tggtgtagct aagcgcgcga cataaaaaaa
    14341 gacgcagacg cgtcacgtgt cgccagtatg gttatatttc ggggttccaa tcccggcacc
    14401 tgattttgca ggtgcgggta aaagataccg ctttcggttg aaaacggcaa ggggaagtcg
    14461 tggaatagag atcgcagatt gttataaata tacacaatag ttaatgttaa caaagacggc
    14521 acatatatta actttactct caatctgctg aatagtttat cgcatgtaat tctcgggtca
    14581 tatagtggat accctccttc ttttctttca tcccctcttg cctcaccata aaagcaaacg
    14641 aaaaaattgc tgaacgagtt atctgaagta aatgctcaaa atggtgaata aaatcacttt
    14701 ctttgataaa aagaatatct tctattagtt gataagattc gtaaatgtcc tcaggtttat
    14761 cacttttatg tacaactaaa aacttatgct ctaaaccatt gcgccattct ttatagaaag
    14821 ccaactcccc acccttatct ttattcaggt cggtagcaat actgtacagc gccagaagcc
    14881 ctggtgattt taccttttca aataattgtc ttctgttatc aacatctaac tgccagaaat
    14941 tctgaaaata gatattcttc tgaggtgtac ctttttttgt tgttgggtaa agattataaa
    15001 gttcacatat ggctaccgca attttatcaa gaataccaaa gcaaagacga aatgccgtcc
    15061 tgattttttc aatttctatt cctaaaactt catcattata caattccaga aaatggcttt
    15121 cgtacttaat gtaatccgta ttttgtggat gaagataatc aaaataaaga tggcgtgcga
    15181 gtgaaaactc cgatttaagg cgatttaata ccatctccat gggaatgata aaatcacctg
    15241 ttacaccgcc tcgcgaagca atcacaagat catctgtagc actccctaca catgggcaat
    15301 acaggccatg ttctgataaa gtcagatgat ttctaagaca aaactgtcga tatgggctta
    15361 acacttcgaa ctcttgttgt gtaagtgctt catcgtgttc gtcagtttca tcatcaacag
    15421 catattctgc gattttctcg ttagtctgcg ctatgcgttc taaatagaac gattcccatt
    15481 gtggtgggat attttttgat acagacgctt taatatatcc tgctctcact tcttttagta
    15541 acttgattga aaatgaagat gaaataagat tgaggaccat aagtgacatc gaacggttga
    15601 cccaagcctg tggtatatct aatcccttaa gattggtaag atcataatat ctcaatgact
    15661 cactgagccg aaattgactt cttaatgaat tagccaaatt aacagatagt tcagcctgaa
    15721 attcctcctg ctcctcagca gatagcttaa aagaacgcca gtagatattt tttaaggaaa
    15781 ccaattcttc tatcgatttg aatgtatggt cattggggtt agtaatgctc accagattag
    15841 atagcgcatt accatagtta taatagtaaa ggcactctcc taacactgat ataaatgctt
    15901 ctttgttatc ttccatcagg ttgcaaccaa gctctgacgc atttttatta ttttgcatct
    15961 gcccgatgtc aacaagagca cccgcaatgt tcgccaatac tataaagtag ttatcgttcc
    16021 tgattgttat tctttccagt ttgttcagga acgcaactga ctgttgataa gattcgtcaa
    16081 atcttccaat atcaaccagt tggctaatga aattactttc atcgacaagt tcttttgtta
    16141 tcaaaataaa ttttccttat acagaggatt tcggtgcttc aacaggaggt atgccctcac
    16201 aggagacatc tataactatc gctgataagc ggtctctttt atcaaccggc acagtttcat
    16261 taagcatatt ttccattgca ctggcgtttt caggttgaga tgtatttttc gccaaatgag
    16321 gcagggaatt cggtcgagca gaaccaaacc aaaaaaccag atagataccg tgaccagcag
    16381 catcggggtt tttagtataa agcttgtcca attgcccatt aagtgcagtc cagacatcac
    16441 gatgataatc acgctttatc tcgataggta tttttatctg cggtagtgaa acaatgatat
    16501 ctgccctttt atcagaaacc atgtgtcctt ctggttcaca gcttattttt tatggattaa
    16561 gccggacacg gagtaaatcc agaaaaatat tacggcaact ctcttcaggt ttaggcgtta
    16621 gggtacggcc atagctgtct tcattccaga attgcttata aatatcggta ttttcattgg
    16681 caattcggtt agaaatatcc cgtatatgat caagcaataa tgagtaaaga tccattatgt
    16741 tggcaggcgt tttattcatc aatgtactta cagtctgc
//
LOCUS       DAJTOR010000081        16624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTOR010000081 DAJTOR010000000
VERSION     DAJTOR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.081"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1706
                     /gene="ldtD"
                     /locus_tag="OO249_002359"
     CDS             <1..1706
                     /gene="ldtD"
                     /locus_tag="OO249_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5636632.1"
                     /translation="EALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLN
                     QLQLLYAARDMQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARD
                     VVLSDAMMGYLHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVA
                     GLAPQHPQYAAMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLD
                     GGPKITLPGDDTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEA
                     VKRFQAWQGLGADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIP
                     AYSLVYYQNGNQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKV
                     RNDPGYLESHGYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKF
                     NMPSSEAIYLHDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDAL
                     KQGDTRYVNIRQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLI
                     R"
     gene            1887..2435
                     /locus_tag="OO249_002360"
     CDS             1887..2435
                     /locus_tag="OO249_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5636633.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPPPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2462..3109
                     /gene="gloC"
                     /locus_tag="OO249_002361"
     CDS             2462..3109
                     /gene="gloC"
                     /locus_tag="OO249_002361"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5636634.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3331..4521)
                     /gene="aspC"
                     /locus_tag="OO249_002362"
     CDS             complement(3331..4521)
                     /gene="aspC"
                     /locus_tag="OO249_002362"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5636635.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARMAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(4706..5794)
                     /gene="ompF"
                     /locus_tag="OO249_002363"
     CDS             complement(4706..5794)
                     /gene="ompF"
                     /locus_tag="OO249_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5636636.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6396..7796)
                     /gene="asnS"
                     /locus_tag="OO249_002364"
     CDS             complement(6396..7796)
                     /gene="asnS"
                     /locus_tag="OO249_002364"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5636637.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(7965..9167)
                     /gene="pncB"
                     /locus_tag="OO249_002365"
     CDS             complement(7965..9167)
                     /gene="pncB"
                     /locus_tag="OO249_002365"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5636638.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9433..12045
                     /gene="pepN"
                     /locus_tag="OO249_002366"
     CDS             9433..12045
                     /gene="pepN"
                     /locus_tag="OO249_002366"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5636639.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12088..12839)
                     /gene="ssuB"
                     /locus_tag="OO249_002367"
                     /pseudo
     CDS             complement(12088..12839)
                     /gene="ssuB"
                     /locus_tag="OO249_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(12836..13573)
                     /gene="ssuC"
                     /locus_tag="OO249_002368"
                     /pseudo
     CDS             complement(12836..13573)
                     /gene="ssuC"
                     /locus_tag="OO249_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            complement(13638..14783)
                     /gene="ssuD"
                     /locus_tag="OO249_002369"
                     /pseudo
     CDS             complement(13638..14783)
                     /gene="ssuD"
                     /locus_tag="OO249_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(14780..15739)
                     /gene="ssuA"
                     /locus_tag="OO249_002370"
     CDS             complement(14780..15739)
                     /gene="ssuA"
                     /locus_tag="OO249_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5636640.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALCIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(15732..16307)
                     /gene="ssuE"
                     /locus_tag="OO249_002371"
     CDS             complement(15732..16307)
                     /gene="ssuE"
                     /locus_tag="OO249_002371"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5636641.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4174 a         4292 c         4215 g         3943 t
ORIGIN      
        1 gcgaggcact gccgcaggcg caagccacgg caataatggc ggggatccag ccattgcctg
       61 aaggtgcggc agaaaaagcc cgcacgcaaa tcgaatctca attacccgca ggttacaagc
      121 cggtttatct taaccagctt caactgttgt atgccgcacg cgatatgcaa cccatgtggg
      181 aaaaccgtga tgctgttaaa gccttccagc aacagctggc agaggtggcg attgccggtt
      241 tccagccgca gtttaataaa tgggtagagt tactgaccga tcctggtgtt aacgggatgg
      301 cacgcgacgt agtgctctct gatgcgatga tgggctatct ccatttcatt gcaaatattc
      361 cggtcaaagg cactcgctgg ctatatagca gtaaacctta tgcacttgca acgccgccgc
      421 tctcggtgat taaccaatgg cagctggcgc tggataaagg tcaattgcct acgtttgttg
      481 caggactggc accgcagcat ccgcaatatg cggcgatgca tgaatcgtta ctggccttac
      541 tcagtgacac caaaccgtgg ccccaactga ccggcaaagc aacgttgcgc ccagggcagt
      601 ggagtaacga cgtaccggcg ttgcgcgaaa tattgcaacg cacaggcatg ttggacgggg
      661 ggccgaaaat tactctacct ggcgatgaca cgccaactga cgcggtagtc agcccatccg
      721 ctgttactgt tgaaacagca gaaactaagc cgatggacaa gcaaacgacg tctcgtagta
      781 aacctgcgcc tgccgttcgc gccgcctacg ataatgaact ggtggaagcc gttaaacgtt
      841 ttcaggcatg gcaaggattg ggggcagatg gtgctattgg cccggcaaca cgtgactggt
      901 taaacgtaac gcccgcccag cgtgctggcg tgttggctct caacatccag cgattgcgct
      961 tgctgccaac agagctttct accgggatca tggttaacat tccggcctat tcgctggtct
     1021 actatcagaa cggcaatcag gtgctggatt cgcgagtcat tgtcggtcgc cccgatcgca
     1081 aaacgccgat gatgagcagt gcccttaaca acgtagtggt aaacccgccg tggaacgtac
     1141 ctccaactct ggcacgcaaa gatattctgc caaaagtgcg caacgatccg ggatatctcg
     1201 aaagccatgg ctatacggtg atgcgtggct ggaacagcag agaagcgatt gacccatggc
     1261 aggttgactg gtctacaatc acggcctcga atttaccgtt ccgcttccag caggctccag
     1321 gcccacggaa ctcgctgggg cgctataaat tcaatatgcc gagttcagag gccatttatt
     1381 tgcatgacac gccgaaccac aatctgttca agcgtgatac acgcgcattg agctcaggct
     1441 gtgtacgagt gaataaagct tccgatctgg cgaatatgct gttgcaggat gcaggctgga
     1501 atgacaaacg tatttctgat gcgctgaagc agggtgatac acgttacgtc aatattcggc
     1561 agtcgattcc ggtgaatctc tactacctga cggcctttgt tggtgcagat ggtcgtaccc
     1621 agtatcgtac agatatttac aattatgatc tgcctgcgcg atccagctcg caaatcgtat
     1681 cgaaagcgga acaattaatc aggtaaatga agtagttcgg ggaaatcagt tgtcgtaata
     1741 attagcatga ttgggggcga ttctctgcag cccccgtcac tgctggggtt gagtcatctt
     1801 gacgtctgct ttacgggcgg ttaaggtgcc tcttgtgcgc cagaagtgca tataaacgat
     1861 aacattgacc tgtagacttg attatcatgg acaaattcga cgctaatcgc cgcaaattgc
     1921 tggcgcttgg tggcgttgca ctcggtgccg ccatcctgcc gccccctgcg tttgcaacac
     1981 tctctacccc acgcccgcgc attttgacac tcaataatct tcataccgga gagtcaatca
     2041 aagcggagtt tttcgatggc agaggctata ttcaggaaga attggcaaaa cttaaccatt
     2101 ttttccgcga ttaccgcgcg aacaaaataa agtccatcga cccaggatta ttcgaccagt
     2161 tgtatcgcct gcaagggttg ttaggcacgc gcaaaccggt gcaactcatt tccggttatc
     2221 gttccattga taccaacaat gaactacgcg cccgcagccg tggagtagcg aagaaaagct
     2281 atcacactaa aggccaggcg atggatttcc atattgaagg tatcgcgtta agcaatattc
     2341 gcaaagccgc gttatctatg cgcgcaggtg gtgtaggata ttacccacgt agtaactttg
     2401 tgcatattga taccgggcca gcacggcact ggtagcaatc gcttaacgaa acaggggcag
     2461 tatgaactat cgtattattc cggtcaccgc attctcccag aactgttcat taatctggtg
     2521 tgaacaaacc cgtctggccg cactggtcga tcctggcggc gatgcggaaa aaatcaaaca
     2581 ggaagttgat gacagcggcc tgacactgat gcagatcctg ctgacgcatg gtcatctgga
     2641 ccacgttggc gcagcggcgg aactggcgca acattacggc gtgccggttt ttggcccgga
     2701 aaaagaagat gagttctggc tgcaaggctt gcctgcgcaa agtcgtatgt ttggtctgga
     2761 agagtgccag ccgctgacgc cagatcgttg gctgaacgaa ggcgatacca tcagcatagg
     2821 aaatgtgact ttacaggtgt tacattgccc tgggcatacg ccgggtcatg tcgtgttttt
     2881 tgatgatcgg gcaaagctgc tgatttctgg cgatgttatt ttcaaaggcg gagtagggcg
     2941 cagtgacttc ccgcgtggcg atcataatca actgatttct tcaatcaaag ataaattgct
     3001 gccactgggg gatgacgtga catttattcc gggtcacgga ccattatcca cacttggtta
     3061 tgaacgcctg cataatccct tcctgcaaga cgaaatgccc gtctggtaag gcacataaaa
     3121 aagcccgctt ttaatgcggg cctggatttc tggcaaagtg cgctttgttt atgccggatg
     3181 cggcacgagc gccttatccg gcctacaaaa tcgtgcaaat tcaaaatatt gcaggggacg
     3241 cgtaggcctg ataagcgtag cgcatcaggc aatgttgcgt ttgtcatcag tctcagcccg
     3301 cttttcagcg ggcttcattg tttttaatgc ttacagcact gccacaatcg cttcgcacag
     3361 cggagccatg ttatctggtg tcatcccggc cacatttacg cgaccagaag caaccgcata
     3421 tacgccaaac tcttcgcgca gacgcagcac ttgttctttt gtcaggccac tgaaggagaa
     3481 catgccgttc tgtttgatga taaagctgaa gtcgcggttt gcgccttttt cctgcagcgt
     3541 attgacgaac aactgacgca tacgctgaat acgctggcgc atatcagtca gctcttgttc
     3601 ccaaatcgca cgtaacgcat cgttgctcag gatggtggca acaacagaag cgccgtgtgc
     3661 tggtgggtta gagtagttag cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac
     3721 ggtttcactg tcggcagcaa ccagagtaca agcgccaaca cgctcgttgt acaggccaaa
     3781 gtttttagag taggaactgg caacaatcag ctctttatgc atagccgcga aagcgcgcag
     3841 tccttcagca tcttcttcca gaccacgggc aaaaccctgg taagcgaagt caaacagcgg
     3901 taaccagcct ttctcaacgg agagttgtgc cagtgtctgc cattgttcca gcgtagggtc
     3961 gataccggtt gggttatggc agcagccatg gaacagcact acgtcgccag cctgagcttc
     4021 attcaggctg ttaatcagtg catcgaagtc aagagtgtga ttttccgcat cataataagc
     4081 gtattcacga acttccagac ctgcagagtt aaagacgctc ttatggttcg gccagcttgg
     4141 gttgctcacc cacactcgct taacgctggt attttttgcc aggaaatcgg cagctacgcg
     4201 tagtgcgcca gtgccccccg gagtctgtgc catgcgagca cgtttgtcat tgatcagggc
     4261 gctaccttta ccaaacagca gttcctgagt gcagcgacca aattcaggga tgccgtcaat
     4321 gccgaggtaa tttttggtgg tttcattttc gagcagatac tgttcagcct ttttcacgct
     4381 ggtcagtacc ggggttttgc ccgtctcatc tttatagaca ccaatcccga ggttaatttt
     4441 gccgggacgt tcatcggcac gaaacagatc ggccaggccc agaatcgggt cggcaggagc
     4501 ggcggtaatg ttctcaaaca tgacgaggtt ccattatggt tacagaaggg aagtccgcta
     4561 tcagggtaac gggagattta caaaattcca actattactg atgaaaacgc aggctgtttt
     4621 tgcaagacgt gagattgctc tggaaggtat aaaaaaaaca ggaccaaagt cctgtttttt
     4681 cggcatttaa caaagaggtg tgctattaga actggtaaac gatacccaca gcaacggtgt
     4741 cgtctgaacc tacgcccagt ttgttatcag aatcgatctg gttgatgatg tagtcaacat
     4801 aggtggacat gtttttgttg aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat
     4861 caacatcacc gataccttct acgtctttcg ctttagattt ggtgtaagcg atggacggac
     4921 gcagaccgaa atcgaactgg tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga
     4981 agccgctggt gtttgtaaat ttattagtga tcggcgtagc gttacgggtt tcaccgtagt
     5041 tcgctgccag gtagatgttg ttcgcgtcgt acttcagacc agtagcccac tgttcagctt
     5101 ttttaccgtt gccaagaggt tgagcttctt gcaggttggt acggtcagct gcaccataag
     5161 caccaacgat accaaagcct tcgtattcgt agctgataga accgccaaca ccgtcgccgt
     5221 tagaacggcg tgcagtgtca cgctcgtttt tacccaggta ctgaacagcg aagttcaggc
     5281 catcaaccag accaaagaag ttggagttac gataggtagc aacgccgcca acacgaccaa
     5341 cgaagaagtc atcgctgtat gcagtatcac caccaaattc tggcagcata tcggtgtaac
     5401 ccagtgcatc ataaaccaca ccgtagttac ggccgtaatc gaaagaacca acgtcagcgt
     5461 atttaagacc cgcgaatgcc agacgcgttt tgttaccagt ttgagcgtca gcgccttcag
     5521 agttgttacc ttggaagtta tattcccact gaccataacc ggtcagatcg gaattgattt
     5581 gagtttcccc tttaaaacca agacgggcat aggtcatgtc gccattgcca ccgtaactgt
     5641 tttcaccgtt acccttggaa aaataatgca gaccaacagc tttaccgtac agatctactt
     5701 tgttgccatc tttgttatag atttctgcag cgtttgcagt acctgctact aacagagcag
     5761 ggacgatcac tgccagaata ttgcgcttca tcattattta ttaccctcat ggtttttttt
     5821 atgacacctg ccactgccgt caataagttc tgtcaataaa aatttacgga actattgatg
     5881 agagtttggt gtctttatgt gtctgcaggc atctttccat tcaaactaac gtttcgctac
     5941 cgtgaaagtg ctgcaaagat aaagatttag tttcaaaaag aaaaaatatg taaccaaaag
     6001 taaaaattaa ggaactttgt gaacaccgtc atatttccat agaaacgtga tgatatttac
     6061 agcaattttt atctatttat atgatttact tatatttaaa ttaactaagc ggaaattttg
     6121 tttctgatgg aaactttatc gacctggcac aaaatcctct ttcagacatc cagaatgcca
     6181 aaaaatagta tgaaaattgt gctatcagct aagaaaaaac taatccgcaa taagaatacg
     6241 ggttattttt tgtgcgtaaa tgttgcaatg taatattccg taacaggatg atcgttatgg
     6301 cggggttgca aatagattgc ttgacttaac aaacagataa cttgacagaa aggataaaaa
     6361 agagccagca ggcgctggct ctttgaggaa gaaaatcaga agctggcgtt gcgcggagta
     6421 cgtgggaacg gaatcacatc acgcacgttt tgcacgccag ttacgtaagc aatcaggcgt
     6481 tcaaaaccaa gaccgaaacc tgaatgcgga acagtaccgt agcgacgcag atcgcgatac
     6541 caccagtaat cttctttatt caggcccatt tccagcatac gctcgtccag aacgtccaga
     6601 cgttcttcac gctgggagcc tccaatgatc tcaccgatgc ccggagccag aacgtccata
     6661 gccgcaacgg ttttaccgtc ttcgttgagg cgcatataga acgctttaat atctttcgga
     6721 tagtttttaa ccactaccgg tgctttaaag tgttcttccg ccagataacg ctcatgctca
     6781 gaagagagat cgacgcccca gtaaaccggg ttttcaaact tcctgccgca gttttcgaga
     6841 atggtcactg cgtcggtgta atccacctgc gcgaaatcgg cctcaatgaa gcgttccaga
     6901 cgtgaaacgg catctttatc tacgcgttca gcgaagaatt tcatgtcgtc tgcgcgttct
     6961 tcgagaaccg ctttgaagac atatttcagc atggcttcag ccagacccgc gatatcgttc
     7021 aggttagcaa acgccacttc cggctccagc atccagaatt ccgccaggtg acggctggtg
     7081 ttggagtttt cagcacggaa agtcgggccg aaggtataaa ttttggataa tgcgcaagcg
     7141 taggtttcgc cgttcaactg gccagatacg gtcaggaaag actctttacc aaagaagtct
     7201 ttgtcgaaat ccactttgcc ctgatcgtta cgcggcaggt tttccagatc cagcgtagaa
     7261 acgcggaaca tttcgcctgc accttcggta tcagatgcgg taatcagtgg cgttgaaacc
     7321 cagaagaatc cctgctcgtt aaagaagcga tgcagcgcct gcgccagcgt atggcgaacg
     7381 cgcgcgacgg caccaatcag gtttgtgcgc ggacgcaggt gagcgacttc acgcagatac
     7441 tcaatgctgt ggcgttttgc cgccatcggg taagtgtctg gatcttcaac ccaaccagca
     7501 acttcaactt tgctggcctg aatttcaaat tgttgcccct ggcccggcga cgccacgact
     7561 ttacccgtca caatgaccga gcagccggtg gtcagacgca ggacgtcttc attgtaattg
     7621 ggcagagaat tattgatgac agcctgtaca ggatcaaagc aggaaccgtc ataaacggcg
     7681 aggaaggaga tgccagcttt tgaatctcgg cgggtacgta cccatccgcg tacggtgact
     7741 tcgctgtcaa cggctacgcg gccctggagt acgtcggcta caggcacaac gctcataata
     7801 ttctctctgt taatagtcgg aaaaaataaa cacttgtcca cccgtaaatg gggggatacc
     7861 tatgttacct ggcatctgca atcagacaag cagaattcgc aaatgcagcg aaagatttcg
     7921 gaattaaagg taaaaaaagg gagctgatta gctccctgat gatattaact ggctttttta
     7981 atatgcggaa ggtcgaacgc tttgcgcagc gcccgaacaa acgctttatc atggcagata
     8041 gttttgccag ggctgtcaga aagtttcgcc accggtttac cgttacactc taccaactta
     8101 atcacaatat tcaggggttt tacctggggg atatcgcagg tcaggcgagt cccaatacca
     8161 aaacttaatt gcacgcggga agagaagtgg cggtatagct caaccgcttt gcgtaaatcc
     8221 agattgtcag agaaaaccag cgttttactc tgtggatcaa ttcccagctt tttataatgt
     8281 gcaatggctt tttcacccca ttcaaccggg tcgccagagt catgacgcag gccctgatac
     8341 cgactggcga actcgacacc gaaatcacgc aggaaagcat ccatagtgat gcagtcggtt
     8401 aatgcaatgc caagttggtc gggatattct tccagccagg cagcaagtgc agctcgctgg
     8461 ctgttggcta ggtccgggct gatttgctga tgtgcctgga accattcgtg cgcctgtgtt
     8521 cccatcggcg tgagggaaag ccgacgcgcc agatcgtagt tgctggtgcc cacgaaccag
     8581 gattcctgtt gcagacgctt aacgatggtt tcttgtactt cgcgagaaaa acggcgacgg
     8641 gtgccaaaat ccatcagatg gaagcgcgac atatcaagac cggcggttaa cgccgagaag
     8701 tcgactaatt tgctttccag cgtgtcgagg gcttgcgcaa cgtcggcctg cggtgagcga
     8761 tagcgatgta ccatttcact gataaccgcc agcaaaggaa cttcccagag gatgacttca
     8821 cgccacggac cgcttaagcg aatatccagc ttgccattat cgttggacac ggtgacttgt
     8881 tccgggttaa agcggaattc gcgtaaccag ttaagatagt cggccttaaa gaaaggcagg
     8941 gcagagagcc actgatattc atcatcctgc aggcgcaggt gctgcatcgc ctgaatctgt
     9001 tcacgaatag catcggcata aatacccagc agatcgtcac ctcggcaacg aaactccgcc
     9061 gcgacatgca catcgtaata gtgatgaaac acggcttgct gcatatgcaa cttataagca
     9121 tctgtatcca gcaacgagtg cagaacagga gaagcgaatt gtgtcatagg tgcgcagtag
     9181 catcctcgta caggagcgtt tagtacaata aacatcttca ggaactgctg gagtatacct
     9241 tgtttcgcaa tttattgaac cccgatcaca ccatatgcca cctttctggt cgatggcatt
     9301 acgcggtgta tgttataaaa atgtagcaat aaaggcgttt gtacctgaaa agatgaagat
     9361 tctgcatagc gcgatttacg caacaggaat agactgaaca ccagactcta taaaagatgc
     9421 taaaggttat ttatgactca acagccacaa gccaaatacc gtcacgatta tcgtgcgccg
     9481 gattaccaga ttactgatat tgacttgacc tttgacctcg acgcgcaaaa gacggtcgtt
     9541 accgcggtca gccaggctgt ccgtcatggt gcatcagatg ctccgcttcg tctcaacggc
     9601 gaagacctca aactggtttc tgttcatatt aatgatgagc cgtggaccgc ctggaaagaa
     9661 gaagagggcg cactggtcat cagtaatttg ccggagcatt ttacgcttaa gatcgttaat
     9721 gaaataagcc cggcggcgaa taccgcgctg gaagggcttt atcagtcagg cgatgcgctt
     9781 tgcacccagt gtgaagccga aggtttccgc catattacgt attatctcga ccgcccggac
     9841 gtgctggcgc gttttaccac caaaattatt gccgataaaa ccaaatatcc cttcctgctt
     9901 tccaacggta accgcgttgc gcaaggtgaa ctggaaaacg gacgccattg ggtacagtgg
     9961 caggacccgt tcccgaaacc gtgctacctg tttgcgctgg tggcaggcga ctttgatgta
    10021 ctgcgcgaca cctttaccac gcgttctggt cgcgaagtgg cgctggagct gtacgtcgat
    10081 cgcggcaacc ttgatcgcgc gccgtgggcg atgacctcgc tgaaaaactc aatgaaatgg
    10141 gatgaagaac gcttcggcct ggagtatgac ctcgacatct atatgatcgt cgcggtggat
    10201 ttcttcaata tgggcgcaat ggagaataag ggtttgaata tctttaactc caaatatgtg
    10261 ctggcccgca ccgacaccgc cactgacaaa gattacctcg atattgaacg cgttatcggc
    10321 catgaatatt tccataactg gaccggtaac cgagtcacct gccgcgactg gttccagctc
    10381 agcctgaaag aaggtttaac cgtcttccgc gatcaggaat tcagctctga ccttggttcc
    10441 cgtgcagtta accgcatcaa taatgtgcgc accatgcgcg gattgcagtt tgcggaagac
    10501 gccagcccga tggcgcaccc gatccgcccg gacatggtca ttgagatgaa caacttctat
    10561 accctgaccg tttacgagaa gggcgcggaa gtgatccgta tgatccacac cctgctgggc
    10621 gaagaaaact tccagaaagg gatgcaactc tatttcgagc gccatgatgg cagcgcggca
    10681 acctgcgacg actttgtgca ggcgatggaa gatgcgtcga atgtcgatct ctcccatttc
    10741 cgccgttggt acagccagtc cggcacgccg attgtgaccg tcaaagacga ctacaatccg
    10801 gaaaccgagc agtacaccct gaccatcagc cagcgcacgc cagccacgcc ggatcaggca
    10861 gaaaaacagc cgctgcatat tccgtttgcc atcgaattgt atgacaacga aggcaaagtg
    10921 atctcgttgc agaaaggcgg tcatccggtg aattccgtgc tgaacgtcac ccaggcggaa
    10981 cagacctttg tttttgataa tgtctacttc cagccggtgc ctgcgctgct gtgcgaattc
    11041 tctgcgccag tgaaactgga atataagtgg agcgatcagc aactgacctt cctgatgcgt
    11101 catgcgcgta atgatttctc ccgctgggat gcggcacaaa gcctgctggc aacctacatc
    11161 aagctgaacg tcgcccgaca tcagcaaggg cagccgctgt ctctgccggt acatgtggct
    11221 gacgctttcc gcgcagtgct gctcgatgag aagattgatc cggcgctggc ggcagaaatc
    11281 ctgacgctgc cttctgtcaa tgaaatggct gaactgttcg atatcatcga cccgattgct
    11341 attgccgaag tacgcgaagc actcactcgt actctggcga ctgaactggc ggatgagctg
    11401 ctggctattt acaacgcgaa ttaccagagc gagtaccgtg ttgagcatga agatattgcg
    11461 aaacgcactc tgcgtaatgc ctgcctgcgc ttccttgctt ttggtgaaac gcatctggct
    11521 gacgtgctgg tgagcaagca gttccacgaa gcgaacaata tgaccgatgc gctggcggcg
    11581 ctttctgcgg cggttgccgc acagctgcca tgccgtgatg cgctgatgca ggaatacgac
    11641 gataagtggc atcaggacgg tctggtgatg gataaatggt ttatcctgca agccaccagc
    11701 ccggcggcga atgtgctgga gacggtgcgc ggtctgttgc agcatcgctc atttaccatg
    11761 agcaacccga accgtattcg ttcgttgatt ggcgcgtttg cgggcagcaa tccggcagcg
    11821 ttccatgccg aagatgggag tggttaccag ttcctggtgg aaatgcttac cgacctcaac
    11881 agccgtaacc cgcaggtggc ttcacgtctg attgaaccgc tgattcgcct gaaacgttat
    11941 gatgccaaac gtcaggagaa aatgcgcgcg gcgctggaac agttgaaagg gctggaaaat
    12001 ctctctggcg atctgtacga gaagatcacc aaagcactgg cttgataaat aaccgaatgg
    12061 cggcaatagc gccgccattc ggggaattta cccctgtttt ctcaggcgaa tttcagattc
    12121 accacgctgc atcacccttt gcaacacttc cgattccagc tccgctaatc gcaccgaccc
    12181 taatcgacgc ggagggggga tatccactgt caaatccaga ccaatttttc cctcttcaat
    12241 taataacacc cggtcagcca ttgctaccgc ttcgctcaca tcatgcgtca ccaacagtac
    12301 ggtaaagccg tgctcctgcc aaagtgacac aatcaaatcc tgcatctcga gtcgcgttaa
    12361 ggcgtccagt gccccgagcg gttcatcaag caacaataaa cccggtcgat gaatcaacgc
    12421 tcttgccagc accactcgct gtttctgccc gcctgaaagt gcggcaggcc attccccggc
    12481 gcgattctcc agtcctaccg cagccaacgc ccgacgcgcg gcatcgcgcc actggccttt
    12541 aaggcctaac ccaacgttat caatcaccga tttccacggt agcagacgcg catcctgaaa
    12601 catcattcgc gtgtcttcct gaatttcagc cagcggtgtg gtgcctgcca acacatcgcc
    12661 tgcggttggc gtttccagcc cggccagcag gcgcagcagg gtacttttgc caccaccgcc
    12721 acaaactgac ctgccggaat atgtaaatcc agttggttca ggacgatatt ttccgcgtaa
    12781 tgtttgctta ctgcattgag caacaatggt gtgccctggt tcagacgagc agtattcata
    12841 ccgtggcctc cttcaaatga taagccgggt tccagcgcaa ccagaggcgc tctaacagct
    12901 gcgcgctgac gtcagcaagt ttgccgagca gggcgtaaag aataatagcg actaccacca
    12961 cgtccgtttg caaaaactct cgcgcattca tcgccagata accaatgccg gaattggcag
    13021 aaatggtttc ggcaacaatc agcgtcagcc acatcaggcc caacgcaaaa cgcacgccga
    13081 ccataattga gggcagggca ccaggcagga tcacatggat aaacagcggt atgccggata
    13141 atccatagct acgcgccatc tccaccagcc cgcgatcgat attacggatg ccatgccagg
    13201 tgttgatata aatgggaaac agcgtaccga gcgccaccag aaagattttt gcggattcat
    13261 caatgccaaa ccacaaaatc accagcggga tcagcgccag atgcggcacg ttgcgcaaca
    13321 tctgaattga ggtatccagc agccgttctc cccagcgaga tagcccgcta atcagtccca
    13381 aaatcaaccc cagcgatccg ccaattgaaa agccaatcag cgcccgctag gaactgatcg
    13441 ccagatgctg ccacagttcg ccgctggcgg agagcgtcca gaacgccgtc accacccctt
    13501 ccggtgaggg caaaatacgc gtcgacagcc agccaactga ggaggccagt tgccacaccg
    13561 ccacgatgcc caccggtaaa aactaggggg caacgcgcaa taaccacttc ttcactggcg
    13621 ttgccattat ttcttcctta gctttgcgcg actttacggg ggataaaatc attcgccacc
    13681 gcttcgcctt gcggattcag cggctgcggc tggggaattt ccggaatggc gacatccaga
    13741 tgtgggaaca gcaactcgcc gacccgatac gcttcttcca gatgcggata gcccgaaagc
    13801 acaaagctgt cgatgccaag cgcggcgtat tcgttgattc gcgcagcgac cgtaggacca
    13861 tcgcccacca gcgccgttcc ggtaccgccg cgcactaagc caacgcccgc ccataaattg
    13921 gggctgatct ccagattgtc gcgcttgccg ttatgtaacg ccgccattcg ctgttgtcct
    13981 acggaatccg tccgggcgaa tgcggcctgt gctttggcga tagtttcatc atcaagatgc
    14041 gagattaacc gctcggcggc ctgccacgct tcgtcgttag tttcacgaac aatcacatgc
    14101 agacgaatac cgaaacgaat tttgcgtcca tgcgcggcag ctttcgcccg cacttgttcg
    14161 atcttctctt ttactagttc tggcggttcg ccccaggtga ggtagagatc aacttgttct
    14221 gccgccagct cctgggcgac gtctgacgat ccgccaaagt aaagcggagg ataaggctgt
    14281 tgaatcggcg ggaagagcag ttttgctccg cgcacatgaa tatgtttacc gttgaaattg
    14341 acggtttccc ccagcaataa gcgccgccag acctaggtaa attccgccga ggcttcgtag
    14401 cgctcgctat gatcaaggaa cacgccgtcg cctgccagct cttgtggatc gctgcctgtg
    14461 accagattaa acaacgcacg accatttgag agacggtcaa gcgtggcggc ctggcgagcg
    14521 gcaacggtag gtgaggttac gctggggcgc agggcgacaa gaaacttcag ccgctgcgtc
    14581 accgggatca tcgacgccgc caccagccac gcatcttcac acgatcgccc cgttgggatc
    14641 agcacaccgg tatagcccag acgatccgcc gcttgcgcaa tttgttgcag ataaccgtga
    14701 tcaaccgggc gtgaaccttc ttccgttccc agatagtgcc cgtcaccgtg ggtcggtaaa
    14761 aaccagaaca tattcagact cataattgtt ttccttccag ttgagtgggc tgccagatgc
    14821 gctggcgaat atcgactttt ttcggcacca gacgattttc ataaaacaga tctgccgttt
    14881 gctgctgtaa ggcggcaacc tcggcgttaa ccggtttgat ggtggtagga gggcgatgat
    14941 ctaagtaaga ggcaatcacc ggtgccggta agcccatcgt ttttgccagt aaagcgatgc
    15001 tttgctcgcg ctggctgcgg gttaacgcat cggcctcact aaaggttgcc agtacgccct
    15061 gaataaaagc gccgtttttt tctgcatagg gacgagctgc cagataaaac gatccagttt
    15121 gattgagatc ggtgccgtct ttcagcaccc gcacgccgcc ctgtaataat gcagcagagt
    15181 agtagggatc ccagatagcc caggcgtcaa cgttaccttg ctggaacgcg gcgcgggcat
    15241 cagcgggcgt caggtaagtg ggttggatat cggtaaactt aagtccggcc tgacgcagtg
    15301 cacgcagtaa aaggttgtgt gaactggaac ctttctgaaa ggcaactttg tgacctttaa
    15361 gatcggctac ggttttgatc gggctgtttt ctgccaccag aatcacttcg gctttgggct
    15421 tcggtggctc gacgcccaca tataccaaat cagccccggc agcctgggca aagattggcg
    15481 gaatatcccc ggtactgccg agatcaatac tgccaacgtt taaggcttcc aacatttgcg
    15541 gacccgcggg gaactccacc caggagattt ttgattgcgg atagcgtttt tccagtaact
    15601 ggtggctttt tgccagtacc ataccaatac tgcctttctg atagcctata cataacgctt
    15661 caggcgagga ttctgcagca accgcaatcg tagagacgct aagcaatccc accagcgcca
    15721 gtttaatgat gttacgcatg ggcattacct cgcagagaca gaaggtcagg aacctgaaca
    15781 tcgcggcggt gcaatgcctg ccagaaagtt tctagcgcgg tatcaagacg ggtttgcaga
    15841 tttggcgtga actggggtct gtgatggtaa tcaattactt gtgagtcatc ggcaaacacg
    15901 ccgtgcagga tctcctgagc tttcagtgcg cttaaaactg gtttaagggc ataatcgacc
    15961 gccagcagat gggccacggt accgcccgtc gccagcggta gcaccacttt gccttgcaaa
    16021 gcgcgctctg gcagcaggtc gagcagggtt ttcagcgcgc cggaataggc ggctttatac
    16081 acaggtgtag cgactatcag tccatcagcc tgttgcagct gttcggtgaa ggtcttgagt
    16141 gccggactat cgaaacgagc ataaagcaga tcttccggga cgaagttttg cagattccag
    16201 tgataaacct ctacatccag gccatttagt ttttcccgcg catattccag caaggagctg
    16261 gagcgagaag gaaagcgagg actacccgcc agggtgatga cacgcatact ctctccttat
    16321 aaccaattgt tctttttttg ttaacattga taacaattcg gtcagtctgt cggagagaca
    16381 agaaaatgcc aaatataaat tttgtgtatc tttttctgaa aataaactaa agaaagggct
    16441 ttatatatca cgcatattta tttgttggat agtcattaga tgttgaatca atgaattact
    16501 cgttcagtta atatttaaat attcatgaaa tctataaatt aaagatttgt cacttattgg
    16561 atttagtatg cttgatttaa atgacggtca attattgtta ttattaagtc taatgtcatt
    16621 taaa
//
LOCUS       DAJTOR010000082        16622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTOR010000082 DAJTOR010000000
VERSION     DAJTOR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.082"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..245)
                     /locus_tag="OO249_002372"
     CDS             complement(<1..245)
                     /locus_tag="OO249_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5636642.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGL"
     gene            352..648
                     /locus_tag="OO249_002373"
     CDS             352..648
                     /locus_tag="OO249_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5636643.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(782..1207)
                     /gene="arsC"
                     /locus_tag="OO249_002374"
     CDS             complement(782..1207)
                     /gene="arsC"
                     /locus_tag="OO249_002374"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5636644.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1220..2509)
                     /gene="arsB"
                     /locus_tag="OO249_002375"
     CDS             complement(1220..2509)
                     /gene="arsB"
                     /locus_tag="OO249_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5636645.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2564..2917)
                     /gene="arsR"
                     /locus_tag="OO249_002376"
     CDS             complement(2564..2917)
                     /gene="arsR"
                     /locus_tag="OO249_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5636646.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            3656..3739
                     /locus_tag="OO249_002377"
     CDS             3656..3739
                     /locus_tag="OO249_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5636647.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3793..5145)
                     /gene="gorA"
                     /locus_tag="OO249_002378"
     CDS             complement(3793..5145)
                     /gene="gorA"
                     /locus_tag="OO249_002378"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5636648.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSEHLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5217..6059)
                     /gene="rlmJ"
                     /locus_tag="OO249_002379"
     CDS             complement(5217..6059)
                     /gene="rlmJ"
                     /locus_tag="OO249_002379"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5636649.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            6262..8304
                     /gene="prlC"
                     /locus_tag="OO249_002380"
     CDS             6262..8304
                     /gene="prlC"
                     /locus_tag="OO249_002380"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5636650.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8312..9064
                     /gene="rsmJ"
                     /locus_tag="OO249_002381"
     CDS             8312..9064
                     /gene="rsmJ"
                     /locus_tag="OO249_002381"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5636651.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(9113..10582)
                     /gene="dtpB"
                     /locus_tag="OO249_002382"
     CDS             complement(9113..10582)
                     /gene="dtpB"
                     /locus_tag="OO249_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR5636652.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINSVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10900..11334)
                     /gene="uspA"
                     /locus_tag="OO249_002383"
     CDS             complement(10900..11334)
                     /gene="uspA"
                     /locus_tag="OO249_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5636653.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11725..12060
                     /gene="uspB"
                     /locus_tag="OO249_002384"
     CDS             11725..12060
                     /gene="uspB"
                     /locus_tag="OO249_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5636654.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12304..13803)
                     /gene="pitA"
                     /locus_tag="OO249_002385"
     CDS             complement(12304..13803)
                     /gene="pitA"
                     /locus_tag="OO249_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5636655.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14035..15237
                     /locus_tag="OO249_002386"
     CDS             14035..15237
                     /locus_tag="OO249_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5636656.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15552..16604)
                     /locus_tag="OO249_002387"
     CDS             complement(15552..16604)
                     /locus_tag="OO249_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HCR5636657.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNILFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGYVIFGIGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILIYLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4270 a         4235 c         4315 g         3802 t
ORIGIN      
        1 aacaatccac gtaacatggg tattgtcgag caactacaga acggggtaac ggctcccaac
       61 agagcagcca ggagatagcc tctccctttt ctggccccca tcatctgctg gattttatgg
      121 tctggcacct tttgtcttat caggctgaca ccggcactaa tcactataaa cagcaacgaa
      181 agctcgacgg cgagaaacac aaacatctct gcggcatctt gcagcatcgc aagccaacta
      241 ctcatacgta acctcattgg ataaatctat atttccagaa taatcgaaat gaaattcaga
      301 tcaagatatt tctggtatta tcgaaatatc aaccagacag aggggtgtca tatgcagttg
      361 gaagaagtcg ctaaagcgct gaaagagcta ggtcacccga cccgtttgtt catatttaaa
      421 catcttgtca aagccgggga acaagggttg ccggtcggtg agttgcaaaa gcagcttggg
      481 atacctggct ccacactcag ccaccatata tcagcactgg tatcggtagg actggtcaaa
      541 caaaaccgtg agagtcggac gctgatgtgt gtttcgcaat acgcaatgct ggaagcgata
      601 attgaattct tacgcgagga gtgctgcgta aatagcaaaa ttgcctgatg cgctacgctc
      661 tacatatttc atgccatata ttgaatttgc acggttttgt aggtcggata agacgcgtta
      721 gcgtcgcatc cgacataaac aaagcgcact ttaccaacta tcggcagtgg atatcgccgc
      781 tttatttcag gcgcttaccc gcttcatcaa ccactttctc gccatcttcc ttggagaatg
      841 cgcctttttg cgcatctggc agaatttcca gcaccacttc tgaagggcgg cacaggcgag
      901 ttcccagcgg cgtcaccaca atcgggcgat taatcagaat cgggtgctga agcataaagt
      961 cgattaaccg atcgtcagta aatttatctt ccgcaaggcc cagctcctca tacggttcga
     1021 cgtttttacg cagcagcgcg cgtacggaaa tccccatatc ggcaatgagt ttgaccagtt
     1081 catcgcgcgt tggcggagtt tccagataat ggataatggt cggttctgtg ccgctgttgc
     1141 ggatcatctc cagcgtatta cgcgacgtgc cgcaggccgg gttgtgataa atggtaatgt
     1201 tgctcatatc agtatctcat tacaaagtga aagagagacg tagcgccagc gcagccagcg
     1261 tcacaaacag cacaggcagg gtcatgataa tccctgtacg gaagtaatat ccccagctga
     1321 tagtcatatt cttctgcgaa agtacgtgca gccagagtag tgtagccagg ctaccaattg
     1381 gggtaatttt cggtcccaaa tcgcagccaa tcacattggc ataaaccatc gcttctttga
     1441 taacgccaga tgccgtgctg ccatcaatgg acaacgcgcc aaccagtacc gtcggcatat
     1501 tgttcataat agaagagagg aaggcggtga ggaatccggt gccgagcgtc gcggcccaca
     1561 ggccgttatc cgccagcacg ttgagtacgc cagaaagata ttccgttaat ccggcattgc
     1621 gcaggccata aaccaccaga tacatgccga gcgagaagat gacaatttgc cagggggcac
     1681 cgcgcaggac tttacccgta ttaatcgcat gaccgcgttt agcgacgaca aataatatca
     1741 gcgcgcccac agctgcaatg gcgctcaccg gaatgccgag cggttccagg acgaaaaatc
     1801 ccaccagcag aagcagtaaa acaacccagc cagttttgaa cgtagcagga tctttgatcg
     1861 cttctgcggg agatttcagc agcgccatat cgtagttctg cggaatatct ttgcgaaaat
     1921 agagatgtag catcaccagc gtggcaacaa tcgcggcgat atccaccggc accatcaccg
     1981 aggcgtattc gcgaaagccg aggccaaaga aatcagcgga aacgatattc accaggttgg
     2041 agacaataag cggcaggctg gcggtatcgg caatgaatcc ggccgccatc acgaacgcca
     2101 gcgtcgtgcc tttactgaat cctaaagcca gcagcatggc gatgacaatc ggcgtcaaaa
     2161 taagcgctgc gccatcattg gcaaacaggg cggcaacggc agcaccgagc aggacaatcc
     2221 aggtaaacag caagcgacca cgaccattac cccagcgtga gacgtgcagc gccgcccatt
     2281 caaaaaagcc ggactcatcc agcagcaggc tgatgataat gacggcgata aacgcagccg
     2341 tcgcgttcca gacgatattc cacaccaccg gaatatcacc cggatggacc acgcccgtaa
     2401 ctaacgctag tactgcgccg agcgttgcac tccagccgat gcctaaacct ttcggctgcc
     2461 agataaccaa tacaatggtc aggacaaaga tagcgcctgc cagtaacata atgcctcctg
     2521 gataaaacac atgcgaaaat acatatgtgt ttagctaaat tttttaactg caaatgttct
     2581 tactgtctgc ggaacagttt tgtcgagcca ggttgcggac aattgcctga atcttttcct
     2641 gttcacatcg ccaggcttca tcaattattt tcgccgccca tgctggaata tgcggcgata
     2701 agcggtaatg aacccattta ccttgcttgc ggtccagcaa tagcccgctt tcacgcagca
     2761 atgccaggtg gcgggagatc ttgggctgcg actggtcgag agcagtgcag agatcgcaga
     2821 cgcataactc tcccagttcg ctgagcagta aaacgatgcc cagacgggtt tcatcagcaa
     2881 gaattttgaa caattggatg ggtaacagaa atgacatatt gcgctcctga ttgttgcagg
     2941 tagtgtctct cttcgaagcg gataagtcaa aaacatatat gatttaacga atgtgtaagt
     3001 gcagaggaag gtaataggtg tgaattttga gttggctatt catttgaaag gaggtctgaa
     3061 tcaggacgta aaaaaaccga ctttgcgtcg gttttttact ttccagccct gagttggtgg
     3121 ctctggctgg agtgagagag ttcttatcta acagctcaat actattagcc agtggctaac
     3181 attgcaagaa ttagcgattt cttcagctgg cggagggagc acttcggtat atatcgcagt
     3241 gacgatttat atactttcac tgggtcatcg tcatattaag ccttgtccga atccggatgt
     3301 aaaaaaccga ctttgcgtcg gttttttact ttccagccct gagttggtgg ctctgactgg
     3361 agtgagatag ccatcatcta acgcatcaat gctaatacca tactgaaact attgcaagga
     3421 cgtgctggtt ttataacctg catgtactgt atgattatcc agttagctct gaggcatttt
     3481 cactctggca atgcgcataa acgctttcaa agtcctggtc agaagtacgg gtggtgccgt
     3541 taactgatgc tctggccgga gtgagagagt tcttatctaa caatgagaca tgcgccgtga
     3601 caggcagtgg atgagtaagc ggatgcattc tcactccatc gcatggagaa aacgggtgat
     3661 tgataaagca atcatcgttc taggggcgtt aattgcgctg ctggaactga tccgctttct
     3721 gcttcagctt ctgaactgat agcggaaacg taattaaggg ctaagagcac actactctta
     3781 gccctttaac atttaacgca ttgtcacgaa ctcttctgcc gccgttgggt gaatggcgac
     3841 ggtattgtcg aagtcttttt tggttgcccc catcttcagc gccaccgcga agccctgcaa
     3901 catttcgtcc ataccaaagc caatgccgtg aataccgaca atcttctctt ccgatccaac
     3961 gcacaccagc ttcatgcggc acggctggcg gtgagtggtg acggcggtat acatcgcggt
     4021 gaaagaggat ttatacactt tcacctgatc gtcgccatac tgctcgcgcg cctgcggttc
     4081 cgttaaacca acagtaccaa tcggcggatg gctgaagacc acggtcggaa tgttgctgta
     4141 atccagatgc tcatccggct tgttattaaa caggtgttca gagagacggc gacccgctgc
     4201 aactgccacc ggtgtcagct ccactgcacc cgtgttatcg cccaccgcgt aaataccttc
     4261 aatgttggtg ttttgatatt tatcgacgac gatatagcct ttttcgttgg ttttaacgcc
     4321 agcggcttcg aggttgatgt tgtcattggc aggctcgcga ccaatcgccc aaatcaggca
     4381 atccaccgtt tcactgcgac catcttccag ctccagcgtc aggctaccat cggcattttt
     4441 cactaccgct ttcgggatgg cgttggtgtg cagctgcggg ccttcggcgt tcatcacttc
     4501 gaccagcgtt tcggaaatca tcgggtcgaa gctgcgcagc ggcgcatgtt tacgcacaaa
     4561 cagatgcgtt ttcgcgccga ggccgttaat cacgcccgcc agctcaacgg cgatgtaacc
     4621 cgcgccaaca accgccacgc gctctggcaa agcaggaagg gcgaagaagc catcagaatc
     4681 aataccgtat tccacgcccg gaatatccgg gtggctcgga cgaccgcctg tggcgatcag
     4741 aatatgatcg gccgtgatgg tttcgccgtt tacctccagc attttggcat caacgaagcg
     4801 ggcaaagcct ttgattacat caacgttatt tttaccgagc acgttttcat aggaagtatg
     4861 aatacggtcg atataggcgg tacggctggc gatcaacgtt tcccagttga acttgttgat
     4921 ggtggtatcg aaaccataat cagggccgta catatggatc gcttcacgga tttgcgccgc
     4981 gtgccacatc acttttttcg gcacgcagcc gacgttcacg caggtgccgc ccagctcttt
     5041 ggcttcaatc agtgcgcatt tctggccgta catagccgcg cggttgatgg aggctatacc
     5101 gccgctgccg ccgccgatgg cgatgtaatc atagtgttta gtcatgacag agtgtcctta
     5161 tcgttgatta ccgcgattgt agcgcgagac gtaataggtg ccagcaatgg ctgcaattac
     5221 tccggcacga tccagcttgc ggtggcgtgc ccggtgcctg ccggaaccag tttgctgtgc
     5281 agccacggca gcacgttatt catctgctgt tccagtttcc acggcgggtt aatcacaatc
     5341 atgccggaag cggtcatgcc acggcgatcg ctgtctggca gtaccgccag ttcaatttgc
     5401 agaattttgc gaataccggt cgcttccaga tcgtggatca tgcgcttaat ttgctgacgc
     5461 agcaccaccg gataccacag tgcgtaagta ccagtggcga aacgtttgta accttctgct
     5521 atcccgctga ccaccgcctg atagtcggtt ttcatttcat acggcgggtc gataaggatt
     5581 aaaccacggc gggaaaccgg cggcagtttg gccttaagct gctggaaacc atcggctttt
     5641 tcgacgcgcg cacggctgtc tttctgaaat tcagaacgca gcaacgggta atcgctcggg
     5701 tgcagttcgg tcagttgcag gctgtcttgt tcgcgcagta gctggcgagc aatcaatggc
     5761 gaaccggggt agtaacgcaa ctgaccgcta cggttgaagt gttttaccac attgatgtac
     5821 gcctccagtt ctgcgggcaa atcgtcctgc tgccagatac gggcgatgcc ttcgagatat
     5881 tcgccggtac gctcggcatg ttcgctgcct aactgataac gcccggcccc tgcgtgggtg
     5941 tcgagataga gaaacggttt atctttctct ttcagcgact cgatgatcag gctctgaacg
     6001 gtatgtttaa ggacgtcggc gtggttgcca gcgtgaaagc tgtggcgata actgagcatg
     6061 ggtaaaggtg ttccggtaag taaaaatcgg cccgtattcg gggcgcacaa aagcgtatca
     6121 ggacagtata ccgaaaagag gccgccgccg cgaaagcgta acgtttctca ttgaaattca
     6181 ctacacttaa ccccatgcta cacacattat gtaaagcgcc tgttgagcgc ttccttaacc
     6241 tctttaacca ggactgcgcg aatgacgaat ccgttactga ctccctttga attgcctccg
     6301 ttttctaaaa ttctcccgga acatgtcgtt ccagccgtga ctcaggcgct gaacgattgc
     6361 cgcgaaaatg tggagcgcgt agtagcgcaa ggggcaccgt acacctggga aaatctctgc
     6421 cagccgttgg cggaagtgga cgatgtgttg gggcgtatct tctccccggt cagccacctg
     6481 aactcggtga aaaatagccc ggaactgcgt gaagcctacg agcaaaccct gccgctgctg
     6541 tcggaataca gcacctgggt agggcaacat gaagggctgt ataaagcgta ccgcgacctg
     6601 cgcgatggcg atcattacgc cacgctgaac acggcgcaga agaaagcggt tgataacgcc
     6661 ctgcgtgatt ttgaactctc tggcataggt ctgccgaaag agaaacagca gcgttacggc
     6721 gaaattgcga cccgtctttc tgaactgggc aaccagtaca gcaacaacgt cctcgatgcg
     6781 acaatgggct ggaccaaact cgttaccgac gaagcggagc tggcggggat gcctgaaagc
     6841 gcgctggctg cggcaaaagc ccaggccgaa gcgaaagagc tggaaggcta cctgctgacg
     6901 ctggatatcc caagctacct gccggtaatg acctactgcg acaaccaggc cttgcgtgaa
     6961 gagatgtatc gcgcttacag cacccgcgcc tccgatcaag gcccgaacgc cggtaaatgg
     7021 gataacagca aggtgatgga agagatcctc gctctgcgtc acgaactggc gcaactgctg
     7081 ggctttgaaa actacgcctt taaatccctt gccactaaaa tggcagaaaa cccgcagcag
     7141 gtgctggatt tcttaaccga tctggcaaaa cgcgcgcgtc cgcaaggcga aaaagagctg
     7201 gcacaactgc gcgccttcgc caaagcggaa tttggcgtcg atgagttgca gccgtgggat
     7261 atcgcgtact acagcgaaaa acaaaaacag cacctctaca gcatcagtga cgaacagctg
     7321 cgtccgtact tcccggaaaa caaagcggtt aacggcctgt ttgaagtggt gaaacgtatt
     7381 tacggcatca ccgctaaaga gcgtaaagat gttgatgtct ggcatccgga tgtacgtttc
     7441 ttcgaactgt atgacgagaa caacgaactg cgcggcagct tctacctcga tctgtatgcc
     7501 cgtgaaaaca agcgcggcgg ggcgtggatg gatgactgcg taggccagat gcgtaaagct
     7561 gacggttctc tgcaaaaacc ggtcgcgtat ttgacttgta acttcaaccg cccggtaaat
     7621 ggtaaaccgg cgctgttcac tcacgacgaa gtgatcaccc tgttccacga gttcggtcac
     7681 ggcctgcacc atatgctgac ccgcatcgaa accgctggtg tttccggtat cagcggtgtg
     7741 ccgtgggatg cggtcgaact gccgagtcag tttatggaaa actggtgctg ggagccggag
     7801 gcgctggcgt ttatctctgg tcactatgaa accggcgaac cgctgccgaa agagttgctg
     7861 gataaaatgc tggcggcgaa gaactaccag gcggcgctgt ttattctgcg ccagctggag
     7921 ttcggcctgt tcgatttccg cctccatgcc gagttccgcc cggatcaggg tgcgaaaatc
     7981 ctcgaaactc tggcagaaat caagaaactg gttgccgtag taccgtctcc atcctggggc
     8041 cgtttcccgc acgctttcag ccatattttc gccggtggtt atgccgcagg ttactacagc
     8101 tacctgtggg ctgacgtact ggcggcagat gccttctcgc gctttgagga agagggcatt
     8161 ttcaaccgtg aaaccgggca gtcgttcctc gacaacattc tgagccgtgg cggttcagaa
     8221 gagccgatgg atctgttcaa acgcttccgt ggtcgtgaac cgcagctgga tgcgatgctg
     8281 gagcattacg gcattaaggg ctgatcattc agtgaaaatc tgcttaattg atgaaacagg
     8341 cgccggagac ggcgccttat ctgttctggc ggcccgctgg gggctggagc acgatgaaga
     8401 caacctgatg gcgctggtgt taacgccgga acatctggaa ttgcgcaagc gtgatgagcc
     8461 aaaacttggc ggcatctttg ttgattttgt tggcggagcg atggcgcacc gacgcaaatt
     8521 cggcggtggt cgcggtgagg cggtggcgaa agcggtgggc attaaaggcg attatttgcc
     8581 ggatgtggtg gacgccaccg cagggctggg gcgcgatgcc tttgtactgg cttcagtcgg
     8641 ctgccgcgtg cggatgctgg agcgtaatcc agtggttgcc gcgctactcg acgacggcct
     8701 ggcgcgtggt tatgcggatg cggaaatcgg cggctggttg caggagcggt tgcagttaat
     8761 tcacgcctcc agcctgacgg cgctaaccga tattacccca cgcccgcagg tggtctatct
     8821 cgatccgatg ttcccgcata agcagaaaag cgcgctagtg aaaaaagaga tgcgtgtctt
     8881 tcagtcgctg gtgggaccgg atcttgatgc cgatggatta ctggagcctg cacgcttgct
     8941 ggcgaccaaa cgcgtggtgg taaagcgccc ggactacgcg ccaccgctag cgaatgtcac
     9001 cacgccaaac gcggttgtga ctaaagggca tcgctttgat atctatgcag gtacgcccgt
     9061 gtaaaagtaa agcagggtag cgataacgct accctgcttt ctgcaagaat aattaatggc
     9121 tttccggcgt cgcaatcatg cgtttcagcc acggcaccat caacagcatc actactgcaa
     9181 cgcccagcgt gaccagacca atcttaccaa acacgttggt atagacgggc aacgtctcaa
     9241 gcggatcggt aatgttgtcc ggcaccgcgg taaatgttgc cacatagccg cccagcaaga
     9301 acgcggcagc ctgcgtcagg aaccacatcc cgagaataaa gcccatcaaa tgctgcggca
     9361 ccagggcagc aatcatcgcc aggccaaggg cgctaataaa cagttcacct aagctctgga
     9421 ataagtacac cagcacgata aaccatggcg atgtcagccc ttgtgcatcc gcaaaccaca
     9481 ttcccgcagc tgccgccgtc aaaaagccca gtgagcacat aaacatgccg agagtaaatt
     9541 tcatcggcat cgagaggtct ttgcctttgt tacccagatg cgtgtaaatg cctgccagta
     9601 ttgggctggc gagtaccacc cagaacgggt taagcgcctg gaagctgacc gagttgatgg
     9661 aaaaaccgag aatttcatga tgcacgttgt tgatggcaaa gaagttcagc gatgttggca
     9721 tctgggcgta gagaatgtaa aacaccaccg cttcgagcat caggacaaag gcgacaaaca
     9781 ttttattgcg cccggtttta tccagcttga atgcctgacg aaagaagatg atggtgacga
     9841 cgatggagag aacaatcagc accagattgg cgacttctac gttgtgcatc agccatgcac
     9901 atacgaagat catcaccacg ctgccaagta atacgtacaa cagtttgctg aagctcatcg
     9961 gcttgaagtc gggttcagaa ccaatgtctt tcaccattcc acgacaggcg atgtaaacca
    10021 gtaatgcgat aattaacccc gccccgcaca ggttgtaggt gactgaataa ccgaatctat
    10081 cagcgatcac aggggccagc gataacgcta tcaacgagcc gatattgatc gacatataga
    10141 acagggtgaa tgcgccatca agccgcggat ctttcggcgg atagcacttc gaaagcaagc
    10201 tggctgggtt agctttaaac aggccgttac cgacagcgat agtccccagg gcgatgaaaa
    10261 tcaggtcagg cttaagtagc gacatgccgg tcatgaagta gccaatcgcc agcacaagtg
    10321 ctccgagaac aatggtgcgt ttggtcccca gcaggtggtc gccgacatag ccgccaatgg
    10381 aaatgaggcc atagaccagc gcagcaaaag caccaaaagt gacaaaagcc tgctcttgcg
    10441 agaatccaag ctgtttaacg aagaaaaccg ccagtacgcc ctgcacgccg tagtagccga
    10501 atcgctccca taactcgaca aaaaagatca tgaaaaatgg gcgaggttgc tgcagcatcc
    10561 ccatgggtgt tgttgtattc atatttatta accttccaat accatcctga aaagtatgaa
    10621 cgcgtaaggg ataacgcgcg aaaaagttta catctgtcag agattcttaa tattgatgcg
    10681 gtgaatggat taatacacta ttacaacaga aaataaccag ctaatcacac taatgatgga
    10741 aaatatgatg gtgttaaatg ttgtattggc aggagatttt gattgcgtac gcagatcgta
    10801 ggccagataa ggggtttacg ctgcatcagg taacaaggcg cgccaaaaag caaaacgccc
    10861 gcatatgcgg gcgttcagga acacgtcgtg gagggaagat tattcttctt cgtcgcgcag
    10921 cggaacaatc agcatatcaa cgtgaacggt gttgatcagc tgacgtgcgg aagacatcag
    10981 tttgctccag aagtcctggt ggtgaccaca aaccaccaga tccatatcgt atttcttgat
    11041 tgcatcgacc agaacctggc ccaggtcgcc gctgccgctc agggtttcag tgattgggta
    11101 gcctgcatta gtggaaagct cggtcagcgc atgatgtgtc tcttcagaga tgcgtttctg
    11161 catatcaccc agattcacat caataagccc ggtgtatagg tcagagtagt ttacatctac
    11221 gtggatcaga gaaactttcg cattgtaggg gcgagccata gagactgctt tctctaccag
    11281 aactttgctt tccggggaga gatcgaccgc gatgagaatg tgtttataag ccatagtgtt
    11341 actccttcca taaagttgtc gatgactggc cagctagcgt ttcttgtgcc gttacgtcac
    11401 ccgcgtcctg cgaactacgt gcgcggggct aacccagccg taaagattca gtgttaagac
    11461 catccttacc ttatagcgac ccggatgatc cgtcaatccg ccttgcttac ccgttagata
    11521 aacaaatttt cgacatgaat atattgatag tggttaacct tctggaaaaa aaacaacctg
    11581 atctcctaca ctatctatag agccgctcgg atgttggtca ccacaaaagc ggtttctcca
    11641 ggacctggtg ttaactgatt ggcagcgtat cgggagcggt agccccgggg tggaactccg
    11701 cgggcaggtc gccggggagg aggtatgata agcaccgtcg cattattttg ggctttatgt
    11761 gtcgtttgca ttgttaacat ggcgcgctat ttctcatcac tacgcgcgtt gttagtggta
    11821 ctgcgtaact gcgatccatt gctctatcaa tatgttgatg gagggggctt ttttacctca
    11881 catggccaac ccaacaaaca ggtgcgtctc gtttggtata tctatgccca acgttatcgc
    11941 gatcatcacg atgatgagtt tattcgccgc tgtgagcggg tgcgtcggca gtttattctg
    12001 actagcgcat tgtgtggtct ggtggtggtc agcctgattg cattgatgat ttggcattaa
    12061 gcacttcagc aataataacg cataaaaaaa gctggccagt aaactgaccc gctcgagact
    12121 gattacaaac gcaaaattgc ctgatgcgct acgcttatca ggcctgcata atctctgcaa
    12181 tctattgaat ttgcatgttt ttgtaggccg aataaggcgt tcacgccgca tcaggcatga
    12241 acaaagcgca ctatgtcaca atctgaagcg ggccagctga cccgctcgtt ttggtgcgta
    12301 cgattacagg aactgcaagg agagccagta cagcccgccg gaaagcagta ccgcagccgg
    12361 aagggtaaac acccaggcca tcagaatgct ggtcacggtt ttacgctgta agccgccacc
    12421 atctaccacc atcgtccccg cgacagaaga ggagagtacg tgagtagtgg aaaccggcat
    12481 cccggtataa ctcgccaggc cgatagacac tgccgccgtc atctgggcag acatcccctg
    12541 agcgtaggtc atgcctttct taccgatttt ctcaccgata gtcgttgcca cacgacgcca
    12601 gccaatcatc gtaccgatac ctaacgccag cgcgaccgcc atgatgatcc acaccggtgc
    12661 atactcgatg gtgctaagca tgtcggactt cagtttcttc aacaggcgct gatcgtcagc
    12721 actcacgcca ggtatcttca ctactttgtc gatagtgtca gaaacgcaca gcataatgcg
    12781 gcgcatctgg ctacgttgat caagcgacag cttgtcgtag ctttccacat cggtagttaa
    12841 catacctttc aggcggttga gcgcgttaat ggtattcgac ggatggcaat ggaactccgc
    12901 gggttgcgtt gcgccagctt ccggagccgg tactaactga tcagcaccgg tagcctgttt
    12961 cagcagcgca ggatgctgct caaagtaagc ttcgacgttg ttgatggcat cacgggtacg
    13021 ggtgatttcg tagccagtgg cattcatatt caccacgaag cctgctggtg cgacaccaat
    13081 caataccaac ataaccagac caatgccttt ctgaccatcg ttcgcgccgt gcgaaaacgc
    13141 cacgccgata gcggaaagga tcagcgcaat acgcgtccag aacggcggct ttttcttgcc
    13201 gtctttcttt tcacgctccg ctggggtcag gtggatacgg gcgcgtttct tggtgccgct
    13261 ccagtagcga cgcagcaaga aaatcagacc gccagcaaac accaggccga caatagggga
    13321 aacaatcaga gaaccgaaaa tacttaatac tttcgggata ttgagtgcat ccaccactga
    13381 cgtcccggtc atcaacgcat tggttaaacc aatcccgatg atcgcgccaa tcagtgtatg
    13441 agagctggat gcaggtaaac caaagtacca ggtacccagg ttccagataa tcgccgccag
    13501 caacatagag aacaccatgg caaggccatg agacgatccc atattaagca gcagatccgt
    13561 cggcagcata tgcacaatgg cataggcaac actcagacca cccagcaaaa cacccaaaaa
    13621 gttgaacacc gccgccataa ccacggcgag ctgagaacgc atcgcgcggg tatagataac
    13681 ggttgccacg gcgttggctg tgtcatggaa accattgatg gcttcgtaga acagcacaaa
    13741 agccagtgca agcaataata acagcccggt atgcaaatcc aggccagcaa acaaatgtag
    13801 cataggacgt tacgccattt tgaggacatg aacgcggcgc attatcagtg actttcgcgg
    13861 cgcgggcaaa gtgaaatata gacttttttt gatttgcctc ctgtatggat ttcactcaaa
    13921 aaataattat cttatataat tcaggcaaat acttcctttt agtaatattg atgctggtgt
    13981 gaccactgag gaatctttac aattcacgct cgttttttct aagaggagcg caacgtggaa
    14041 aggtttgatg ccattattat aggcgctggt gcggcgggta tgttctgttc tgcgctggca
    14101 ggtcaggcag gacgccgggt tctgctgatc gataatggta aaaaaccagg gcgcaaaatc
    14161 cttatgtctg gcggtgggcg ctgcaacttt accaaccttt atgtcgaacc aggcgcttat
    14221 ctgagccaga atccgcattt ttgtaagtct gcactcgcgc gttttaccca gtgggatttc
    14281 attgatctgg tcaataaaca cggcatcgcc tggcacgaga aaacgttagg acaactcttc
    14341 tgcgatgact ccgcgcagca gattgtcgac atgctggtgg atgagtgcga gaagggcaat
    14401 gtaaccttca gattgcgtag cgaagtgctg agtgtggcga aggatgaaac aggcttcacg
    14461 cttgaactga acggcatgac tgtcggttgc gaaaagctgg tcatcgcgac cggtgggctg
    14521 tcaatgccgg ggctgggcgc atcgccgttt ggttataaga ttgccgaaca atttggcctc
    14581 aacgtgctgc cgacccgtgc gggtctggtg ccattcactc tgcataaacc gttgctcgaa
    14641 gagttacagg tgctggcggg cgtggcggtg ccttccgtga ttaccgctga aaacggcacc
    14701 gttttccgtg agaacttact cttcacccac cgcggcttat ctggaccggc ggtgttgcag
    14761 atttccagct actggcaacc gggtgaattt gtcagtatca atctgctacc ggatgtggac
    14821 ctcgaaacct tcctgaatga gcagcgtaac gcacatccga atcagagcct gaaaaacaca
    14881 ctggcggttc atctaccgaa gcggttggtt gaacgcttac agcaactcgg gcaaatcccg
    14941 gatgtttcgc taaaacagct caacgtgcgt gaccaacagg cactgattag cacattgacc
    15001 gactggcgcg tacaacccaa cggcactgaa ggctatcgca ctgccgaagt gacgctcggc
    15061 ggcgtggaca ccaacgaact ctcttcacgg acgatggaag cgcgcaaagt gcctgggctg
    15121 tacttcatcg gcgaagtgat ggacgttacc ggctggctgg ggggctataa cttccagtgg
    15181 gcgtggtcga gtgcgtgggc ttgtgcgcag gatttgattg cagcgaagtc gtcctgacct
    15241 gttacctaaa gatgaaaatt attagagccg cattaaaaat gagctaattt tgatagtggc
    15301 tatcttgtga ttattttcta atgagcccgt gaactgaaac cctccaggct taatataagg
    15361 tggaaggaaa gatgattgaa aactcaccca gtggccagat ctttataaaa tatgaacagg
    15421 ctgaaaaaga cagtaacttt gttgtttttt atccttaacc actatgcatt aatgctgcgt
    15481 tatttcatga tgcctaagaa aaaccagcgt tacgcaaatg gtcaacgctg gttttatccg
    15541 gtacgttgca attatttttt agcagaacct gcttctaata ttgaaacgat tgagaacaac
    15601 gtaaagcata ccgctcccaa accgaccaaa acccgcgaag gaataaaata tcccatgtcg
    15661 gtctgcgcca tttccgcaag aaacgatgcc aggaaaaggc aaaacaggca ggtgaagacg
    15721 ggaatcatcg gtattctgtt ggctaacgaa caggtacgtc tccataccag tgccagtagc
    15781 cagacttttg agaatatgct gtaacagatc attccgaggt aaataagaat aatgccggga
    15841 gccagtcggg cgcttgcatc ggaactgact aagacgtata tcccctgcag taccgtgatt
    15901 gagccgagaa aaataaccca ataacaccac agccagtgtt ctttagttga aaacgtatta
    15961 cgtgtttgat ggacaattgt ggcaacaagg ccaattagac aggcacagat tgcggttaac
    16021 ccaagcaata cgtgacccgc gacataatgc ggagttatgt cagcactacg taacagcgta
    16081 atagaccaaa tgaaaccgag cagggtgagt aaaacgggaa cggcaatgag gcagttaccg
    16141 attaatgaag aataagcctg taccggtgtt ccgtcgctct tgctccccgc tgcatttttg
    16201 ggaatgagca gaaagtgacc ggacgacgct gccaccgtcg atacacatgc ggcaatcata
    16261 ccaatgccga aaataacata gccggcgaca aactcgtctg ccatcacatc attgcctgct
    16321 aacagtgccc aaccccatat catggtgata attgaccctg catagccaat aatggggaac
    16381 aatatattgt aaaacgtatt aacgccgcgc gtgagctgcg aaatgataat aaatgcggta
    16441 gtgaataatg ccaggcatat ggctgccaga gaaatcagca catgacccgc gacgaaatac
    16501 tcgctttgcc ctgaactttc cagcacaaag ccacctaacg cgatacaaat taagcccata
    16561 atcaaaggga ttaatttgaa aagccacccg atatatatgt tcactttaaa ctcctcaggt
    16621 at
//
LOCUS       DAJTOR010000083        16468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTOR010000083 DAJTOR010000000
VERSION     DAJTOR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.083"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(124..1407)
                     /gene="gltA"
                     /locus_tag="OO249_002388"
     CDS             complement(124..1407)
                     /gene="gltA"
                     /locus_tag="OO249_002388"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5636658.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1613..1720)
                     /locus_tag="OO249_002389"
     CDS             complement(1613..1720)
                     /locus_tag="OO249_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636659.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2101..2505
                     /gene="sdhC"
                     /locus_tag="OO249_002390"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2101..2505
                     /gene="sdhC"
                     /locus_tag="OO249_002390"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5636660.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2499..2846
                     /gene="sdhD"
                     /locus_tag="OO249_002391"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2499..2846
                     /gene="sdhD"
                     /locus_tag="OO249_002391"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5636661.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2846..4612
                     /gene="sdhA"
                     /locus_tag="OO249_002392"
     CDS             2846..4612
                     /gene="sdhA"
                     /locus_tag="OO249_002392"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5636662.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4628..5344
                     /gene="sdhB"
                     /locus_tag="OO249_002393"
     CDS             4628..5344
                     /gene="sdhB"
                     /locus_tag="OO249_002393"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5636663.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5645..8446
                     /gene="sucA"
                     /locus_tag="OO249_002394"
     CDS             5645..8446
                     /gene="sucA"
                     /locus_tag="OO249_002394"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5636664.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8461..9678
                     /gene="odhB"
                     /locus_tag="OO249_002395"
     CDS             8461..9678
                     /gene="odhB"
                     /locus_tag="OO249_002395"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5636665.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9954..11120
                     /gene="sucC"
                     /locus_tag="OO249_002396"
     CDS             9954..11120
                     /gene="sucC"
                     /locus_tag="OO249_002396"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5636666.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11120..11989
                     /gene="sucD"
                     /locus_tag="OO249_002397"
     CDS             11120..11989
                     /gene="sucD"
                     /locus_tag="OO249_002397"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5636667.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12093..12815)
                     /gene="mngR"
                     /locus_tag="OO249_002398"
     CDS             complement(12093..12815)
                     /gene="mngR"
                     /locus_tag="OO249_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5636668.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12984..14900
                     /gene="mngA"
                     /locus_tag="OO249_002399"
     CDS             12984..14900
                     /gene="mngA"
                     /locus_tag="OO249_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5636669.1"
                     /translation="MNLTTLTHRDALRLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVPACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMVNGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14918..>16468
                     /locus_tag="OO249_002400"
     CDS             14918..>16468
                     /locus_tag="OO249_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5636670.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPTDENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYD"
BASE COUNT         3951 a         4130 c         4486 g         3901 t
ORIGIN      
        1 ttttagaata tatgaatgtc cgatagtgga ttttattttt acaatttttt gtgtttaggc
       61 atataaaaat taacccgcca tttgaacggc gggttaaaat atttacaact tagcaatcaa
      121 ccattaacgc ttgatatcgc ttttaaagtc gcgtttttca tatcctgtat acagctgacg
      181 cggacgggca atcttcatac cgtcactgtg catttcgctc cagtgggcga tccagccaac
      241 ggtacgtgcc attgcgaaaa tgacggtgaa catggaagac ggaataccca tcgctttcag
      301 gatgatacca gagtagaaat cgacgttcgg gtacagtttc ttctcgataa agtacgggtc
      361 gttcagcgcg atgttttcca gctccatagc cacttccagc aggtcatctt tggtgcccag
      421 ctctttcaga acttcatggc aggtttcacg cattacggtg gcgcgcgggt cgtaattttt
      481 gtacacgcgg tgaccgaagc ccatcaggcg gaaagaatca tttttatctt tcgcacgacg
      541 aacaaattcc ggaatgtgtt taacggagct aatttcttcc agcattttca gcgccgcttc
      601 gttagcacca ccgtgcgcag gtccccacag tgaagcaata cctgctgcga tacaggcaaa
      661 cgggttcgca cccgaagagc cagcggtacg cacggtggag gtagaggcgt tctgttcatg
      721 gtcagcgtgc aggatcagaa tacggtccat agcacgttcc agaatcggat taacttcata
      781 cggttcgcac ggcgtggaga acatcatatt caggaagtta ccggcatagg agagatcgtt
      841 gcgcggataa acaaatggct gaccaatgga atacttgtaa cacatcgcgg ccatggtcgg
      901 cattttcgac agcaggcgga acgcggcaat ttcacgatga cgaggattgt taacatccag
      961 cgagtcgtga tagaacgccg ccagcgcgcc ggtaatacca cacatgactg ccattggatg
     1021 tgagtcgcga cggaaagcgt ggaacagacg ggtaatctgc tcgtggatca tggtatgacg
     1081 ggtcaccgta gttttaaatt cgtcatactg ttcctgagtc ggtttttcac cattcagcag
     1141 gatgtaacaa acttccaggt agttagaatc ggtcgccagc tgatcgatcg ggaaaccgcg
     1201 gtgcagcaaa ataccttcat caccatcaat aaaagtaatt ttagattcgc aggatgcggt
     1261 tgaagtgaag cctgggtcaa aggtgaacac accttttgaa ccgagagtac ggatatcaat
     1321 aacatcttga cccagcgtgc ctttcagcac atccagttca acagctgtgt ccccgttgag
     1381 ggtgagtttt gcttttgtat cagccattta aggtctcctt agcgccttat tgcgtaagac
     1441 tgccggaact taaatttgcc ttcgcacatc aacctggctt tacccgtttg ttatttggct
     1501 cgccgctctg tgaaagaggg gaaaacctgg gtacagagct ctgggcgctt gcaggtaaag
     1561 gatccattga tgacgaataa atggcgaatc aagtacttag caatccgaat tattaaactt
     1621 gtctaccact aataactgtc ccgaatgaat tggtcaatac ttccacactg ttacataagt
     1681 taatcttagg tgaaataccg acttcataac ttttacgcat tatatgcttt tcctggtaat
     1741 gtttgtaaca actttgttga gtgattgtca aattagatga ttaaaaatta aataaatgtt
     1801 gttgtcgtga cctggatcac tgttcaggat aaaacccgac aaactatatg taggttaatt
     1861 gtaatgattt tgtgaacagc ctatactgcc gctaggtctc cggaacaccc tgcaatcccg
     1921 agccacccag cgttgtaacg tgtcgttttc gcatctggaa gcagtgtttt gcatgacgcg
     1981 cagttataga aaggacgctg tctgacccgc aagcagaccg gaggaaggaa atcccgacgt
     2041 ctccaggtaa cagaaagtta acctctgtgc ccgtagtccc cagggaataa taagaacagc
     2101 atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa gacctgttaa tctggaccta
     2161 cagaccatcc ggttccccat cacggcgata gcgtccattc tccatcgcgt ttccggtgtg
     2221 atcacctttg ttgcagtggg catcctgctg tggcttctgg gtaccagcct ctcttcccct
     2281 gaaggtttcg agcaagcttc cgcgattatg ggcagcttct tcgtcaaatt tatcatgtgg
     2341 ggcatcctta ccgctctggc gtatcacgtc gtcgtaggta ttcgccacat gatgatggat
     2401 tttggctatc tggaagaaac attcgaagcg ggtaaacgct ccgccaaaat ctcctttgtt
     2461 attactgtcg tgctttcact tctcgcagga gtcctcgtat ggtaagcaac gcctccgcat
     2521 taggacgcaa tggcgtacat gatttcatcc tcgttcgcgc taccgctatc gtcctgacgc
     2581 tctacatcat ttatatggtc ggttttttcg ccaccagtgg cgaactgaca tatgaagtct
     2641 ggatcggttt cttcgcctct gcgttcacca aagtgttcac cctgctggcg ctgttttcta
     2701 tcttgattca tgcctggatc ggcatgtggc aggtgttgac cgactacgtt aaaccgctgg
     2761 cattgcgcct gatgctgcaa ctggtgattg tcgttgcact ggtggtttac gtgatttatg
     2821 gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc agagaatttg atgcagttgt
     2881 gattggtgcc ggtggcgcag gtatgcgcgc ggcgctgcaa atttcccaga gcggccagac
     2941 ctgtgcgctg ctctctaaag tcttcccgac ccgttcccat accgtttctg cgcaaggcgg
     3001 cattaccgtt gcgctgggta atacccatga agataactgg gaatggcata tgtacgacac
     3061 cgtaaaaggg tcggactata tcggtgacca ggacgcgatt gaatatatgt gtaaaaccgg
     3121 gccggaagcg attctggaac tcgaacacat gggcctgccg ttctcgcgtc ttgatgatgg
     3181 tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac ttcggcggcg agcaggcggc
     3241 acgcactgcg gcggcggctg accgtaccgg tcacgcactg ttgcacacgc tttatcagca
     3301 gaacctgaaa aaccacacca ccattttctc cgagtggtat gcgctggatc tggtgaagaa
     3361 ccaggatggc gcggtggtgg gttgtactgc actgtgcatc gaaactggtg aagtggttta
     3421 tttcaaagcc cgcgctaccg tgctggcgac tggcggggca gggcgtattt atcagtccac
     3481 cactaacgcc cacattaaca ctggcgacgg cgtcggcatg gctatccgcg caggcgtacc
     3541 ggtgcaggat atggaaatgt ggcagttcca cccgaccggt attgccggtg cgggcgtact
     3601 ggtcaccgaa ggttgccgtg gtgaaggcgg ttatctgctg aacaaacatg gcgaacgctt
     3661 tatggagcgt tatgcgccga acgccaaaga cctggcgggc cgtgatgtgg tggcgcgttc
     3721 catcatgatc gaaatccgtg aaggtcgcgg ctgtgatggt ccgtgggggc cacacgcgaa
     3781 actgaaactc gaccacctgg gtaaagaagt tctcgaatcc cgtctgccgg gcatcctgga
     3841 actctcccgt accttcgctc acgtcgatcc ggtgaaagag ccgatcccgg ttatcccaac
     3901 ctgtcattac atgatgggcg gtattccgac caaagtgacc ggtcaggcgc tgactgtgaa
     3961 tgagaaaggc gaagatgtgg ttgttccggg actgtttgcc gttggtgaaa tcgcttgtgt
     4021 atcggtacac ggcgctaacc gtctgggcgg caactcgctg ctggacctgg tggtctttgg
     4081 tcgtgcggca ggtctgcatc tgcaagagtc tatcgccgag cagggcgcat tgcgcgatgc
     4141 cagcgagtct gatgttgaag catctctgga tcgtctgaac cgctggaaca ataatcgtaa
     4201 cggtgaagat ccggtggcga ttcgtaaagc gctgcaagaa tgtatgcagc ataacttctc
     4261 ggtcttccgt gaaggtgatg cgatggcgaa agggcttgag cagttgaaag ttatccgcga
     4321 gcgtctgaaa aatgcccgtc tggatgacac ttccagcgag ttcaacaccc agcgcgttga
     4381 gtgcctggaa ctggataacc tgatggaaac ggcgtatgca acggctgttt ctgccaactt
     4441 ccgtaccgaa agccgtggcg cgcatagccg cttcgacttc ccggatcgtg atgatgaaaa
     4501 ctggctgtgc cactccctgt atctgccaga gtcggaatcc atgacgcgcc gaagcgtcaa
     4561 catggaaccg aaactgcgcc cggcattccc gccgaagatt cgtacttact aatgcggaga
     4621 caggaaaatg agactcgagt tttcaattta tcgctataac ccggatgttg atgatgctcc
     4681 gcgtatgcag gattacaccc tggaagcgga agaaggtcgc gacatgatgc tgctggatgc
     4741 gcttatccag ctgaaagaga aagatcccag cctgtcgttc cgccgctcct gtcgtgaagg
     4801 tgtgtgcggt tccgacggtc tgaacatgaa cggcaagaat ggtctggcct gtattacccc
     4861 gatttcggca ctcaaccagc cgggcaagaa gattgtgatt cgcccgctgc caggtttacc
     4921 ggtgatccgc gatttggtgg tagacatggg acaattctat gcgcaatatg agaaaattaa
     4981 gccttacctg ttgaataatg gacaaaatcc gccagctcgc gagcatttac agatgccaga
     5041 gcagcgcgaa aaactcgacg ggctgtatga atgtattctc tgcgcatgtt gttcaacctc
     5101 ttgtccgtct ttctggtgga atcccgataa gtttatcggc ccggcaggct tgttagcggc
     5161 atatcgtttc ctgatcgata gccgtgatac cgagactgac agccgcctcg acggtttgag
     5221 cgatgcattc agcgtattcc gctgtcacag catcatgaac tgcgtcagtg tatgtccgaa
     5281 ggggctgaac ccgacgcgcg ccatcggcca tatcaagtcg atgttgttgc aacgtaatgc
     5341 gtaaaccgta ggcctgataa gacgcgcaag cgtcgcatca ggcaaccagt gccggatgcg
     5401 gcgtgaacgc cttatccggc ctacaagtca ttacccgtag gcctgataag cgcagcgcat
     5461 caggcgtaac aaagaaatgc aggaaatctt taaaaactgc ccctgacact aagacagttt
     5521 ttaaaggttc cttcgcgagc cactacgtag acaagagctc gcaagtgaac cccggcacgc
     5581 acatcactgt gcgtggtagt atccacggcg aagtaagcat aaaaaagatg cttaagggat
     5641 cacgatgcag aacagcgctt tgaaagcctg gttggactct tcttacctct ctggcgcaaa
     5701 ccagagctgg atagaacagc tctatgaaga cttcttaacc gatcctgact cggttgacgc
     5761 taactggcgt tcgacgttcc agcagttacc tggtacggga gtcaaaccgg atcaattcca
     5821 ctctcaaacg cgtgaatatt tccgccgcct ggcgaaagac gcttcacgtt actcttcaac
     5881 gatctccgac cctgacacca atgtgaagca ggttaaagtc ctgcagctca ttaacgcata
     5941 ccgcttccgt ggtcaccagc atgcgaatct cgatccgctg ggactgtggc agcaagataa
     6001 agtggccgat ctggatccgt ctttccacga tctgaccgaa gcagacttcc aggaaacctt
     6061 caacgtcggt tcatttgcca gcggcaaaga aaccatgaaa ctcggcgagc tgctggaagc
     6121 cctcaagcaa acctactgcg gcccgattgg tgccgagtat atgcacatca ccagcaccga
     6181 agaaaaacgc tggatccaac agcgtatcga gtctggtcgc gcgactttca atagcgaaga
     6241 gaaaaaacgc ttcttaagcg aactgaccgc cgctgaaggc cttgaacgtt acctcggcgc
     6301 aaaattccct ggcgcaaaac gcttctcgct ggaaggcggt gacgcgttaa tcccgatgct
     6361 taaagagatg atccgccacg ctggcaacag cggcacccgc gaagtggttc tcgggatggc
     6421 gcaccgtggt cgtctgaacg tgctggtgaa cgtgctgggt aaaaaaccgc aagacttgtt
     6481 cgacgagttc gccggtaaac ataaagaaca cctcggcacg ggtgacgtga aataccacat
     6541 gggcttctcg tctgacttcc agaccgatgg cggcctggtg cacctggcgc tggcgtttaa
     6601 cccgtctcac cttgagattg taagcccggt agttatcggt tctgttcgtg cccgtctgga
     6661 cagacttgat gagccgagca gcaacaaagt gctgccaatc accatccacg gtgacgccgc
     6721 agtgaccggg cagggcgtgg ttcaggaaac cctgaacatg tcgaaagcgc gtggttatga
     6781 agttggcggt acggtacgta tcgttatcaa caaccaggtt ggtttcacca cctctaatcc
     6841 gctggatgcc cgttctacgc cgtactgtac tgatatcggt aagatggttc aggccccgat
     6901 tttccacgtt aacgcggacg atccggaagc cgttgccttt gtgacccgtc tggcgctcga
     6961 tttccgtaac acctttaaac gtgatgtctt catcgacctg gtgtgctacc gccgtcacgg
     7021 ccacaacgaa gccgacgagc cgagcgcaac ccagccgctg atgtatcaga aaatcaaaaa
     7081 acatccgaca ccgcgcaaaa tctacgctga caagctggag caggaaaaag tggcgacgct
     7141 ggaagatgcc accgagatgg ttaacctgta ccgcgatgcg ctggatgctg gcgattgcgt
     7201 agtggcagag tggcgtccga tgaacatgca ctctttcacc tggtcgccgt acctcaacca
     7261 cgaatgggac gaagagtacc cgaacaaagt tgagatgaag cgcctgcagg agctggcgaa
     7321 acgcatcagc acggtgccgg aagcagttga aatgcagtct cgcgttgcca agatttatgg
     7381 cgatcgccag gcgatggctg ccggtgagaa actgttcgac tggggcggtg cggaaaacct
     7441 cgcttacgcc acgctggttg acgaaggcat tccggttcgc ctgtcgggtg aagactccgg
     7501 tcgcggtacc ttcttccacc gccacgcggt gatccacaac cagtctaacg gttccactta
     7561 cacgccgctg caacacatcc ataacgggca gggcgcgttc cgcgtctggg actccgtact
     7621 gtctgaagaa gcagtgctgg cgtttgaata cggttatgcc accgcagaac cacgcactct
     7681 gaccatctgg gaagcgcagt tcggtgactt cgctaacggt gctcaggtgg ttatcgacca
     7741 gttcatctcc tctggcgaac agaaatgggg ccggatgtgt ggtctggtga tgttgctgcc
     7801 gcacggttat gaagggcagg ggccggagca ctcctccgcg cgtctggaac gttatctgca
     7861 actttgtgct gagcaaaaca tgcaggtgtg cgtaccgtct accccggcac aggtttacca
     7921 catgctgcgt cgtcaggcgc tgcgcgggat gcgtcgtccg ctggtcgtga tgtcgccgaa
     7981 atccctgctg cgtcatccgc tggcggtttc cagcctcgaa gaactggcga acggcacctt
     8041 cctgccagcc atcggtgaaa tcgacgagct tgatccgaag ggcgtgaagc gcgtagtgat
     8101 gtgttctggt aaggtttatt acgacctgct ggaacagcgt cgtaagaaca atcaacacga
     8161 tgtcgccatt gtgcgtatcg agcaactcta cccgttcccg cataaagcga tgcaggaagt
     8221 gttgcagcag tttgctcacg tcaaggattt tgtctggtgc caggaagagc cgctcaacca
     8281 gggcgcatgg tactgcagcc agcatcattt ccgtgaagtg attccgtttg gggcttctct
     8341 gcgttatgca ggccgcccgg cctccgcctc tccggcggta gggtatatgt ccgttcacca
     8401 gaaacagcaa caagatctgg ttaatgacgc gctgaacgtc gaataaataa aggatacaca
     8461 atgagtagcg tagatattct ggtccctgac ctgcctgaat ccgtagccga tgccaccgtc
     8521 gcaacctggc ataaaaaacc cggcgacgca gtcgtacgtg atgaagtgct ggtagaaatc
     8581 gaaactgaca aagtggtact ggaagtaccg gcatcagcag acggcattct ggatgcggtt
     8641 ctggaagatg aaggtacaac cgtaacgtct cgtcagatcc ttggtcgcct gcgtgaaggc
     8701 aacagcgccg gtaaagaaac cagcgccaaa tctgaagaga aagcgtccac tccggcgcaa
     8761 cgccagcagg cgtctctgga agagcaaaac aacgatgcgt taagcccggc gatccgtcgc
     8821 ttgctggctg aacataacct cgacgccagc gctattaaag gcaccggcgt gggtggtcgt
     8881 ctgacccgtg aagatgtgga aaaacatctg gcgaaatccc cggcgaaaga gtctgcaccg
     8941 gcagcggctg ctccggcggc gcaaccggct ctggctgcac gtagtgaaaa acgtgtcccg
     9001 atgactcgcc tgcgtaagcg tgtggcagag cgtctgctgg aagcgaaaaa ctccaccgcc
     9061 atgctgacca cgttcaacga agtcaacatg aagccgatta tggatctgcg taagcagtac
     9121 ggtgaagcgt ttgaaaaacg ccacggcatc cgtctgggct ttatgtcctt ctacgtgaaa
     9181 gcggtggttg aagccctgaa acgttacccg gaagtgaatg cttctatcga cggcgatgac
     9241 gtggtttacc acaactattt cgacgtcagc atggcggttt ctacgccgcg cggcctagtg
     9301 acgccggttc tgcgtgatgt cgataccctc ggcatggcag acatcgagaa gaaaatcaaa
     9361 gagctggcag tcaaaggccg tgatggcaag ctgaccgttg aagatctgac cggtggtaac
     9421 ttcaccatca ccaacggtgg tgtgttcggt tccctgatgt ctacgccgat catcaacccg
     9481 ccgcagagcg caattctggg tatgcacgct atcaaagatc gtccgatggc ggtgaatggt
     9541 caggttgaga tcctgccgat gatgtacctg gcgctgtcct acgatcaccg tctgatcgat
     9601 ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt tgctggaaga tccgacgcgt
     9661 ctgctgctgg acgtgtagta gtttaagttt cacctgcact gtagacagga taaggcatta
     9721 tcgccttctc cggcaattga agcctgatgc gacgcttaac gcgtcttatc aggcctacgg
     9781 gaagcaccaa tgtaggtcgg ataaggcgct tgcgccgcat ccgacaagcg atgcctgatg
     9841 cgacacttac gcgtcttatc aggcctacag gtgaccgaca atgcccggag gcgatacgaa
     9901 atattcggtc tacggtttaa aagataacga ttgctgaagg atggacagaa cacatgaact
     9961 tacatgaata tcaggcaaaa caactttttg cccgctatgg cttaccagca ccggtgggtt
    10021 atgcctgtac tactccgcgc gaagcagaag aagccgcttc aaaaatcggt gccggtccgt
    10081 gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa agcgggcggt gtgaaagttg
    10141 taaacagcaa agaagacatc cgtgcttttg cagaaaattg gctcggcaag cgtctggtaa
    10201 cgtatcaaac agatgccaat ggccaaccgg ttaaccagat tctggttgaa gcagcgaccg
    10261 atatcgctaa agagctgtat ctcggtgccg ttgtagaccg tagttcccgt cgtgtggtct
    10321 ttatggcctc cactgaaggc ggcgtggaaa tcgaaaaagt ggcggaagaa actccgcagc
    10381 tgatccataa agttgcgctt gatccgctga caggcccgat gccgtatcag ggacgcgagc
    10441 tggcgttcaa actgggtctg gaaggtaaac tggttcagca gttcaccaaa atcttcatgg
    10501 gtctggcgac cattttcctg gagcgcgacc tggcgttgat cgaaatcaac ccgctggtca
    10561 tcaccaaaca gggtgatctg atttgcctcg acggcaaact gggcgctgat ggcaacgcac
    10621 tgttccgcca gcttgatctg cgcgaaatgc gtgaccagtc gcaggaagat ccgcgtgaag
    10681 cacaggctgc acagtgggaa ctgaactacg ttgcgctgga cggtaacatc ggttgtatgg
    10741 ttaacggcgc aggtctggcg atgggtacga tggacatcgt taaactgcac ggcggcgaac
    10801 cggctaactt ccttgacgtt ggcggcggtg caaccaaaga acgtgtaacc gaagcgttca
    10861 aaatcatcct ctctgacgac aaagtgaaag tcgttctggt taacatcttc ggcggtatcg
    10921 ttcgttgcga cctgatcgct gacggtatca tcggcgcggt agcagaagtg ggtgttaacg
    10981 taccggtcgt ggtacgtctg gaaggtaaca acgccgaact cggcgcgaag aaactggctg
    11041 acagcggcct gaatattatt gcagcaaaag gtctgacgga tgcagctcag caggttgttg
    11101 ccgcagtgga ggggaaataa tgtccatttt aattgataaa aacaccaagg ttatctgcca
    11161 gggctttact ggtagccagg ggactttcca ctcagaacag gccattgcat acggcactaa
    11221 aatggttggc ggcgtaaccc caggtaaagg cggtactacc cacctcggcc tgccggtgtt
    11281 caacaccgtg cgtgaagctg ttgctgctac tggcgctacc gcttctgtta tctacgtacc
    11341 agcaccgttc tgcaaagact ccattctgga agctattgat gcaggcatca aactgattat
    11401 caccatcact gaaggcatcc cgacgctgga tatgctgact gtgaaagtga aactggacga
    11461 agccggcgtt cgtatgatcg gcccgaactg cccgggcgtt atcactccgg gtgaatgcaa
    11521 aatcggtatc cagccgggtc acattcacaa accgggtaaa gtgggtatcg tttcccgttc
    11581 cggtacactg acctatgaag cggttaaaca gaccacggat tacggtttcg gtcagtcgac
    11641 ctgtgttggt atcggcggtg acccgatccc aggctctaac tttatcgaca tcctcgaaat
    11701 gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc ggtgagatcg gcggtagcgc
    11761 tgaagaagaa gcggcagcgt acatcaaaga gcacgttacc aagccagttg taggttacat
    11821 cgctggtgtg actgcgccga aaggcaaacg tatgggccac gcgggtgcga tcattgccgg
    11881 tgggaaaggg actgcggatg agaaattcgc tgctctggaa gccgcaggcg tgaaaaccgt
    11941 tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt ctgaaataaa tatctgtaat
    12001 aagaaatagc cctcgccgct tccctctaca ggaatggcga agggctgtcg gtttcgacat
    12061 ggttggccat cgtatgatgg ccttttttgt gcttatcgcg atgattttcg ctgcgctatc
    12121 agggtaaatt tatagtcatc ggtattaaaa gcgttgcggc tatattcaaa cacccgacca
    12181 tcaactaaat atccacgcga tactttttca agaatcggct ttgtctggct gatattaagc
    12241 agacggctca tctcttcggt tggcatcaga ggaatgattt cctgttcgct acgatcgata
    12301 accatcttct tcacttcttc aataaagtga tatttcgaat tttccatgac ctgccaggtg
    12361 agatccggga acaacgcaag cggcatccag gtttcttcca gcgccattgg cttttgcttg
    12421 cgatagcgca cgcgcttcac atgccacaca cgatcctgcg cggtgatttg tagctgttgc
    12481 tgaagaaaat cgtcagccgg aatcacttca aatatcagga cttcactgtg tgtatcgacg
    12541 tgacggtccg acagtttttc atcaaaactg gttaactgaa aaatatcgta attgacccgc
    12601 tcttctttga cgtaagtccc gctgccctga atgctttcga ggatctgctg ctcgactagc
    12661 tggcgcaaag cctgacgcac cgtaacccgg ctgacgccaa actctgtttg tagcgctgat
    12721 tcagtgggta acgcatcgcc aggtttaagc tcgccacgcg caatttgttc acgaatgcga
    12781 tcggcaatct gccggtataa gggcttgtgt cccattttta gtatctcatt aatacgaatt
    12841 tagccattat gcccgataaa ttcatcctgt aaataatgca aatacaatac aaataatttc
    12901 aatcaagtga aattgatcat ataatggtat tgttttatcg ggcacactgg cgcgactata
    12961 aaaacgatca agtgaggatc atgatgaatc tgacgactct gacccaccgc gatgcgttgc
    13021 gtctgaatgc gcgctttacc agccgtgaag aggccatcca cgcgttgact caacgtcttg
    13081 ctgctctggg gaaaatttcc agtactgagc aatttctgga agaagtgtat cgccgtgaaa
    13141 gccttggccc gaccgcctta ggtgaagggt tggctgtgcc gcatggcaaa actgctgcgg
    13201 taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc gcttcagtgg gaaggcgttg
    13261 atggcccgga agcagttgat ttagtggtgc tgctggcgat tccccccaat gaagcgggta
    13321 ctatgcatat gcaactgctg acagcgctga ccacgcgcct tgcggatgat gagattcggg
    13381 cgcgtataca gtcggcgacg acgcctgatg agttgctctc ggcgctggat gacaagggag
    13441 gcacgcaacc ttctgcctct ttttccaacg cgccaactat cgtctgcgta ccggcctgtc
    13501 cggcgggtat tgctcacacc tatatggctg cggaatatct ggaaaaagcc ggacgcaaac
    13561 tcggcgtaaa tgtttacgtt gaaaaacaag gcgctaacgg cattgaaggg cgtttaacgg
    13621 cggatcaact caatagtgca accgcctgta tttttgcggc tgaagtcgcc atcaaggaga
    13681 gtgagcgttt taatggcatt cccgcgcttt cagtgcctgt tgccgagccg attcgccatg
    13741 cagaagcgtt gatccaacaa gcgcttaccc tcaagcgtag cgatgagacg cgtaccgtac
    13801 agcaagatac gcaaccggtg aaaagtgtca aaacggagct gaaacaggca ctgttgagcg
    13861 gaatctcttt tgccgtaccg ttgattgtcg cggggggcac ggtgctggcg gtcgcggtat
    13921 tactgtcgca aatcttcggg ctacaagatc tgtttaatga agaaaactcc tggctgtgga
    13981 tgtaccgcaa gctgggcggc gggctgctcg gaattttgat ggtaccggtg ctcgcggcct
    14041 atactgccta ttctctggca gataaaccgg cgttagcgcc aggctttgcg gctggacttg
    14101 ccgccaacat gatcggctcc gggtttctcg gcgcggtcgt tggcggattg atagccggtt
    14161 acttgatgcg ctgggtgaaa aatcacttgc gtcttagcag taaattcaat ggattcctga
    14221 ctttttatct ctacccggtg ctcggtacgt tgggagcggg cagtctgatg ctgtttgtgg
    14281 tgggagaacc tgtcgcatgg atcaataact cgcttaccgc ctggctgaac ggtctgtcag
    14341 gaagtaacgc gctgttgctg ggtgccattt tcggttttat gtgttccttt gatcttggag
    14401 ggccagtgaa caaagccgct tatgcattct gcctgggcgc aatggtgaac ggcgtttacg
    14461 gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc atttaccgta accgcttcca
    14521 cgatgctcgc accgcgcctg tttaaagagt ttgaaattga gaccgggaaa tccacctggc
    14581 tgttagggct ggcaggtatt accgaagggg cgatcccgat ggcgattgaa gatccgctgc
    14641 gggttattgg ttcgtttgtg ctgggctcta tggtaacggg cgctattgtc ggtgcgatga
    14701 atatcggcct ttcgacaccc ggtgccggca ttttctcgct ctttttactt catgataatg
    14761 gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc ggcattggtg ggggctgcaa
    14821 tctcgactgc aattctcctg atctggcggc gtcacgcggt taagcatggc aactatctga
    14881 ctgatggcgt aatgccataa acaaaaagga aacgacgatg aaagcggtat ctcgtgttca
    14941 catcaccccg catatgcact gggatcgaga gtggtatttc accaccgaag agtcacgtat
    15001 tctgctggtc aataatatgg aagagatcct gtgccgactg gaacaggaca acgaatacaa
    15061 atattacgta ctcgacgggc aaacggcgat cctcgaagat tatttcgcgg tgaaaccgga
    15121 aaacaaagac cgtgtgaaga aacaggtaga agccggcaag ttgattatcg gcccctggta
    15181 tacccagacc gataccacga ttgtttctgc ggaatccatc gtccgtaatc tgatgtacgg
    15241 aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata ggttatttac cagattcctt
    15301 tggcatgtcc gggcaactgc cgcatatcta caatggattt ggcattaccc gcaccatgtt
    15361 ctggcgcgga tgttcggagc gccacggtac tgataaaacc gagtttttgt ggcaaagcag
    15421 tgacggtagc gaagtgacgg cgcaggtgct gccgctgggc tacgccatag gtaagtactt
    15481 acctaccgac gaaaacggat tacgtaaacg cctcgacagt tattttgacg tgctggaaaa
    15541 agcgtctgta accaaagaga ttttgctgcc gaatggtcat gaccagatgc cattgcagca
    15601 aaatatcttc gaagtgatgg ataagctacg tgagatctac cctcaacgta agtttgtgat
    15661 gagccgcttt gaagaggtat ttgagaagat cgaagcgcag cgtgaaagtc tggccaccct
    15721 gaaaggtgaa tttatcgatg gcaaatatat gcgcgtacat cgcaccatcg gttccacgcg
    15781 catggatatc aaaattgccc acgcacgtat tgaaaataag attgttaatc tgctggaacc
    15841 gctggcaacg ctggcctgga cgttgggttt tgaataccac cacggcttgc tggagaaaat
    15901 gtggaaagag atcttaaaaa atcatgccca cgacagtatc ggttgttgtt gcagtgacaa
    15961 agttcatcgc gaaattgtcg cccgcttcga actggctgaa gacatggcgg ataatctgct
    16021 gcgtttctac atgcgcaaaa ttgccgataa catgccacag agtgatgccg acaaactcgt
    16081 gctgtttaac ctgatgccct ggccgcggga agaagttatt aacaccactg tgcggctgcg
    16141 cgccagccag tttaatctac gagacgatcg cggtcagcct gtaccgtatt ttattcgcca
    16201 tgcccgtgag atcgatccag gcctaatcga tcggcaaata gttcattacg gtaattacga
    16261 tccctttatg gagtttgata tacagatcaa ccagattgtc ccttctatgg gctatcgcac
    16321 gctttatatc gaagcgaatc agcctggcaa cgtgattgcg gcaaaaagtg acgctgaagg
    16381 gatactggaa aatgctttct ggcaaattgc gctcaatgag gatggttctc tgcaactggt
    16441 agataaagac agcggtgtgc gctatgac
//
LOCUS       DAJTOR010000084        16358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTOR010000084 DAJTOR010000000
VERSION     DAJTOR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.084"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..305)
                     /gene="hlyE"
                     /locus_tag="OO249_002401"
     CDS             complement(<1..305)
                     /gene="hlyE"
                     /locus_tag="OO249_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCR5636671.1"
                     /translation="MTEIVADKMVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLF"
     gene            678..1097
                     /locus_tag="OO249_002402"
     CDS             678..1097
                     /locus_tag="OO249_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5636672.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1097..2364
                     /gene="umuC"
                     /locus_tag="OO249_002403"
                     /pseudo
     CDS             1097..2364
                     /gene="umuC"
                     /locus_tag="OO249_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(2410..2940)
                     /gene="dsbB"
                     /locus_tag="OO249_002404"
     CDS             complement(2410..2940)
                     /gene="dsbB"
                     /locus_tag="OO249_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5636673.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(3086..4627)
                     /gene="nhaB"
                     /locus_tag="OO249_002405"
     CDS             complement(3086..4627)
                     /gene="nhaB"
                     /locus_tag="OO249_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5636674.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4849..5568
                     /gene="fadR"
                     /locus_tag="OO249_002406"
     CDS             4849..5568
                     /gene="fadR"
                     /locus_tag="OO249_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5636675.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5620..7152)
                     /locus_tag="OO249_002407"
     CDS             complement(5620..7152)
                     /locus_tag="OO249_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5636676.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAKARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7482..8780
                     /gene="dadA"
                     /locus_tag="OO249_002408"
     CDS             7482..8780
                     /gene="dadA"
                     /locus_tag="OO249_002408"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5636677.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8790..9849
                     /gene="dadX"
                     /locus_tag="OO249_002409"
                     /pseudo
     CDS             8790..9849
                     /gene="dadX"
                     /locus_tag="OO249_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(10236..11972)
                     /locus_tag="OO249_002410"
     CDS             complement(10236..11972)
                     /locus_tag="OO249_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5636678.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGTAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(12067..12981)
                     /gene="ldcA"
                     /locus_tag="OO249_002411"
     CDS             complement(12067..12981)
                     /gene="ldcA"
                     /locus_tag="OO249_002411"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5636679.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVSAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            13081..13692
                     /gene="emtA"
                     /locus_tag="OO249_002412"
     CDS             13081..13692
                     /gene="emtA"
                     /locus_tag="OO249_002412"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5636680.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13694..14428)
                     /locus_tag="OO249_002413"
     CDS             complement(13694..14428)
                     /locus_tag="OO249_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5636681.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14629..14883
                     /locus_tag="OO249_002414"
     CDS             14629..14883
                     /locus_tag="OO249_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5636682.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGLFRLLRRE"
     gene            15062..15502
                     /locus_tag="OO249_002415"
     CDS             15062..15502
                     /locus_tag="OO249_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636683.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPAV"
     gene            complement(15581..16358)
                     /locus_tag="OO249_002416"
                     /pseudo
     CDS             complement(15581..16358)
                     /locus_tag="OO249_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
BASE COUNT         4130 a         4039 c         4236 g         3953 t
ORIGIN      
        1 tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa caccacacca ttcatacact
       61 gtttgggttg cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg
      121 actaaaacgg aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt
      181 tcatcaaagg tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct
      241 ccatctgcgg tttcgattgc gtttttaact acttctacca ttttatctgc aacgatttca
      301 gtcataatca ttcgcctctt taaatatata aattgtaatg aaactcctgt tttacaacta
      361 ttaataaatt ttacttcatc taattcatag ttagccgggt gggatgcgtc aatgtcttta
      421 tttctattaa tatgataaat atcaaacaat gtttaatgtc attatggcga atgcttctat
      481 tctatttttt tgccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg
      541 ccacgtgagc acaggataag agaacgaaaa atcagcagtc tatacagcga caaatattga
      601 tagcctgaat cagtattgat ctgctggcaa gaatagacta ctgtatataa aaacagtata
      661 acttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt
      721 ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggcagca gattacgtcg
      781 aacagcgcat cgatctgaat caactgttga tccagcatcc cagcgcgact tacttcgtca
      841 aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta ctgattgtcg
      901 atagcgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac ggcgagttta
      961 cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac agtgcgtact
     1021 cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg
     1081 ttaaggcgat gcgctgatgt ttgctctctg tgatgtaaac gcgttttatg ccagctgtga
     1141 gacggtgttt cgccctgatt tatggggtaa accggtggtt gtactatcga ataatgacgg
     1201 ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggt gttaaaatgg gcgatccctg
     1261 gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca gcaattatga
     1321 gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat cgccccgcgt
     1381 cgagatttac agtattgatg aggcattctg cgatctgaca ggtgtgcgta attgtcgcga
     1441 tctgactgat tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt
     1501 tggtgtgggg atcgcccaga ccaaaacgct ggctaagctt gccaatcatg cggcaaaaaa
     1561 atggcagcgg cagacggggg ggtggtggat ttatcaaatc tggaacgcca gcgtaaatta
     1621 atgtctgctc tccccgtgga tgacgtctgg gggattggac ggcggatcag caaaaaactg
     1681 gacgcgatgg ggatcaaaac cgttcttgat ttggcggata cagatatccg gtttatccgt
     1741 aaacatttta atgtcgtgct cgaaagaacg gtgcgtgaac tgcgcggtga accctgtttg
     1801 caactggaag aatttgcacc gacaaagcag gaaattatct gttcccgctc gtttggtgaa
     1861 cgtatcacgg attatacgtc gatgcggcag gccatttgta gttacgctgc ccgggcggcg
     1921 gaaaaacttc gcagtgagca tcaatattgt cggtttatct ccacatttat taagacgtca
     1981 ccatttgcgc tcaatgaacc ttattacggc aatagcgcgt cggtaaaact gctgacgccc
     2041 actcaggaca gcagggatat cattaacgcc gctacgcgat ctctggatgc catctggcaa
     2101 gcgggccatc gctaccaaaa agcgggcgtg atgctggggg atttcttcag tcagggagtc
     2161 gcgcagctca atttattcga tgacaacgca ccgcgccccg ggagtgagca attgatgacg
     2221 gtaatggata cactgaatgc taaagagggc agaggaacac tctattttgc cgggcagggg
     2281 atccaacaac aatggcagat gaagcgagcc atgctttcac cacgttatac aacgcgaagt
     2341 tctgatttac tgcgggtcaa ataaatatag cggcaggaaa aaagtgctcc cgcaggagcg
     2401 ccgaatggat tagcgaccga acagatcacg ttttttcgct ttaaacggct gggaaatcat
     2461 caccagcacc gcgacaatca ggtaagcgat aaaaataccg agcagccact gcggcatttc
     2521 cagacctaaa aaatcccact gacgctcggc gcaatcgcca gaggcgacaa acacttgcgg
     2581 cacccattta tccagcggca gccattccgg gaaacgaacc ataaaatcac aggtggcaaa
     2641 cggcgaagga tagagctgaa gcatggtgtg ctcgtaagtt aactgcacac cgcggaacgc
     2701 actatacaac cagataacca tcgctacata acgcagcgga gttttcgggg cgatcgcgcc
     2761 aatcagcgca gcacccagaa cgccgaataa cgcgcagcgt tcataaatac agagcacgca
     2821 aggtttcagt aacatcacat gctggaacca cagcgccgtc agttccagtg ccagagcagt
     2881 aaacgccatc aacagccacg cgccccggcc ttgtgaacat tggttcaaaa atcgcaacat
     2941 aatcatttcc ctgcaatatg catagagtgc gcagtttaaa ccaattcatt cgctgcgcca
     3001 ccaggaggca gcaaaaatag gacgcaattg gacaattatc gggcgaaaag gcaaaccggg
     3061 catttatatg cccggtaagt tgttatcaaa gcgttgctat ccagcccatt tgcataaacc
     3121 attcggttac aggggcaagc gtaaactcga cgcagagcag cccgacgagt gtcaggacga
     3181 gggtgtaagg cagggccatc cacaccatgc ggccataaga gaggcgaatc aatggcgcga
     3241 gtgcagaggt cagcaggaac aggaacgcag cctgaccgtt cggcgtagcg acagagggca
     3301 gattggtacc ggtattaatg gcgaccgcca gcagctcgta ttgcttcaac gtgatagcgc
     3361 cactttccat tgccgctttc gcttcgttga tataaatcgt ccccacgaag acgttatccg
     3421 aaatggatga cagcagaccg ttgaaaatat agaacaacga cagctgagca tgttccgatg
     3481 cctgcaacac aaactgaata atcggcgaaa acagttgttg gtcgataatc accgcgacta
     3541 ccgagaaaaa caccgtcaac agtgcggtga atggcagaga ttcggtgaag gctttaccga
     3601 tagcatgttc atcggtgaca ccggtcaatg atgtcgccag aataatgacc gacaaaccaa
     3661 tcaagccaac ctccgccaga tgcaacgcca gcgcagtcac cagccagacg ccgataatcg
     3721 cctggacaat cagacggatt ttatcctgac gggtacgctg gtggcggctt tgatcgtcaa
     3781 actgttgcaa cacttcgcgg actttctccg gcagcgtttc accgtagcca aaccaacgca
     3841 gcttctctac cagcaggcag gttaacaggc cacaaatcag aaccggaacg gtcaccggcg
     3901 acatgcgcag gaagaaatcg ccaaaatgcc agccagccgc tttagcgatg atcaggttct
     3961 gtggttcgcc caccatggtc attacgccgc ctaatgcggt gccgacaccg gcatgcatca
     4021 tcaggctacg cagaaagcca cggaactgtt ccagaaccac tttgtaatgc ttgtcgatat
     4081 gactatcgtc ttgcaggtcg gtatcttcag tacgggaaga ggctacgcgg tgataaatac
     4141 cataaaaacc gactgcaacg ctgatcacca ccgccacgac ggttaaggca tcgaggaacg
     4201 cggagaggaa cgccgccgcc acgcaaaaag agagcgacag cagcattttg gaacgaatgc
     4261 ttaacagcaa acgggtaaat atgaacagca atagctgttt cataaaatag atacccgcca
     4321 ccataaacat cagtaacagc aagacttcaa gatttgccgc cacctcttca cggacgtgtt
     4381 ccgcgctggt catgccgatg aataccgctt caatagccaa cagaccaccg gggagtagcg
     4441 ggtagcattt cagggccatc gccagagtga aaataaattc cgctaccagc aaccagccag
     4501 cgacgaaagg gctgatgagg aaaattaacg gatttacgat taagaaaatg atgagggcga
     4561 gtttgtacca gtcgggggac tggcccaaaa aattgcgcca tagcgcgcgg ccccaggaga
     4621 tctccatgat ggtttccctt accttacaaa taatcaatga tgtttttatg tttaaacgca
     4681 aagcttaacg gtcaggcagg agtgaggcaa gtcttgatag tcaaggggaa agagatgcgg
     4741 aaaatgaagc cttgatccct tttttcttct ttttgtctgc tatcagcgta gttagccctc
     4801 tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat ggtcattaag
     4861 gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg gaataaccgc
     4921 ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat tggcgtaacg
     4981 cgtactacgt tacgtgaagt gttacagcgt ctggcacgag atggctggtt gaccattcaa
     5041 catggcaagc cgacgaaggt gaataatttc tgggaaacct ctggtttaaa tatccttgaa
     5101 acactggcgc gactggatca cgaaagtgtg ccgcagctta ttgataattt gctgtcggtg
     5161 cgtaccaata tttccactat ttttattcgc accgcgtttc gtcagcatcc cgataaagcg
     5221 caggaagtgc tggctaccgc taatgaagtg gccgatcacg ccgatgcctt tgccgagctg
     5281 gattacaaca tattccgcgg cctggcgttt gcttccggca acccgattta cggtctgatc
     5341 cttaacggga tgaaagggct gtatacgcgc attggtcgtc actatttcgc caatccggaa
     5401 gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag tgaaggcgcg
     5461 cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggcga gatttggcac
     5521 cggatgcaga aaaatctgcc gggtgattta gccattcagg ggcgataatc ccttccgttt
     5581 aaaaagcaaa cccctcaaac gaggggtttt ttgttgtttt tacagatttc ccattcttgg
     5641 cgggcaacgt tccagcaact cgacgctgcc atcttcgttt tgctgttcca gcatcacatc
     5701 aaatccccac aggcgatgca catgcttcag cacttctttg cgcccccgat ccagcggtgc
     5761 gcgattatgt ggaatgtaac gcagcgtcag cgaacggtcg ccgcgcaaat ccacgttcca
     5821 gatctgaata ttcggctcca gattacttaa gttatattgc gacgataacc ggttacggat
     5881 ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat gccgatcgtc
     5941 atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagaa actggctgat
     6001 aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc agtcggaacc
     6061 ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa tccgtttaat
     6121 atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc acgggctgtt
     6181 ataggggggc tggaagacca cattggtgtg gctgtgcaaa aactccagca taaaacgttc
     6241 cgttactttc ccttcatcat acagatggtt aaggatggtg tagtgccaga aggtggccca
     6301 gccttcgttc atcacctgag tctgtttttg cggataaaaa tactggctca ccttacgcac
     6361 aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct ccataaaata
     6421 gagcaggttt tcttgtggtt cggacggata gcggcgcgct ttagcaaccg ttttctcttc
     6481 ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgtagat actcttcgcg
     6541 acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt tgtaccggtc
     6601 cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt catccacgcc
     6661 ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat cgacaatcga
     6721 actggcgtcg gtccaactgc ggaataagta attgttttta aagaaagagt tatgcccata
     6781 gcaggcgtgt gccatcacca gcgcttgcat ggtaatggtg ttctcttcca tcaggtaagc
     6841 gatacacggg ttagagttaa tgacgatttc ataggccagt ccttgctgac cgtgcttata
     6901 cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt taattggcat
     6961 gccgacgctg gagtaggcat ccatcatctg ttctgaggtt atcacttcaa tctggtgcgg
     7021 gtaggtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat aaacatccag
     7081 caggtcgaac gtccagtcgg gtccatcgct caaccgtgtg gtgtccttat tcatagaatc
     7141 gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag cacctcattt
     7201 caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt tgcgatttct
     7261 tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat aaaaagggcg
     7321 ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg tgagccagct
     7381 caccataaaa aagctgtatg ttgaataata ttttcaactg agttatcaag atgtaattag
     7441 attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt gtcatactgg
     7501 gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga catgaggtca
     7561 ccgtcattga tcgggagccg ggggccgccc tggaaaccag cgccgcgaat gccgggcaaa
     7621 tctcccccgg atatgctgca ccgtgggcgg caccaggtgt gcctttaaaa gcgatcaaat
     7681 ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag tttcagttga
     7741 aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa aacaaagggc
     7801 ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc gccgaaacca
     7861 atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc gaacaacagt
     7921 atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg tatcagctgc
     7981 tggaatccag ccgcctggcg gaagtggagc cagcgctggc agaagtagcg cacaaactga
     8041 cgggtggtct gcaactgccc aatgatgaaa ccggagactg ccagctgttt acccagaatc
     8101 tggcgcggat ggcagagcag gcgggggtta aattccgctt taatacgccc gttgaccaac
     8161 tgctttgcga cggcgagcaa atctacggcg tgaagtgtgg cgatgaagtg attaaggccg
     8221 atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc attgttgata
     8281 ttccggtgta tccgctgaaa ggctactcgc tgactattcc aattgcgcag gaagatggtg
     8341 cgccagtatc caccattctt gatgaaacct acaaaatcgc cattacccgt ttcgataacc
     8401 gcattcgtgt tggcggaatg gcggaaattg ttggttttaa caccgagctg ttgcaaccgc
     8461 gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc acgcggcggt catgtcgagc
     8521 aggcgacttt ctggacgggt ttgcgcccga tgacgccaga cggtacgccg gttgtcgggc
     8581 gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc tggacgatgg
     8641 cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca gcgatcccat
     8701 atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt ccgggccatt
     8761 tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc agcctcgatc
     8821 tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac gcgggtggta
     8881 aaagcgaacg cttacgggca tggtattgag cgtatctgga gcgcgctcgg ggccaccgat
     8941 ggctttgcat tacttaacct ggaagaggca ataacgttac gtgagcgcgg ctggaagggg
     9001 ccgatcctga tgctggaagg atttttccat gctcaggatc tggagattta tgaccagcac
     9061 cgcctgacca cctgcgtaca cagcaactgg cagctcaaag cactgcaaaa tgcgcggcta
     9121 aaagcaccgt tggatattta tcttaaagtg aacagtggga tgaatcggtt gggcttccag
     9181 cccgatcgcg tgcttaccgt ctggcagcag ttgcgggcaa tggcgaatgt tggcgagatg
     9241 accctgatgt cgcattttgc cgaagcggaa catcctgatg gaatttccag cgcgatggcg
     9301 cgtattgagc aggcggcgga agggctggag tgtcggcgtt cgttgtccaa ttcggcggcg
     9361 actctgtggc acccggaagc gcattttgac tgggttcggc ctggcattat tttgtatggc
     9421 gcttcgccgt ccggtcagtg gcgtgatatc gccaataccg gattacgtcc ggtgatgacg
     9481 ctaagcagtg agattattgg tgtccagacg ctaaaagcgg gtgagcgtgt gggctacggc
     9541 ggtcgctata ctgcgcgcga tgaacagcga atcggcattg tcgccgcagg gtacgccgac
     9601 ggttatccgc gccacgcgcc taccggtacc cctgttttag tggacggcgt gcgcaccatg
     9661 acggtgggga ccgtctcgat ggatatgctg gcggtcgatt taacgccttg cccgcaggcg
     9721 ggtattggta cgccggttga gctgtggggc aaggagatca aaattgatga tgtcgccgcc
     9781 gctgccggaa cggtgggcta tgagttgatg tgcgcgctgg cgctacgcgt cccggttgtg
     9841 acggtgtaac ttgttgtaag ccggatcgga ggcaacgtct tctgggtgca aaaaaaatca
     9901 tccatccggc tggtcagcaa ctgtagttgt taatgtgaca gagccattgc ccatgatagt
     9961 gtccattaaa aggatggaca ctatttcccc ggaacctgaa ctcaccgcac aggcgttcta
    10021 cataaaacgc ttacgcttca ttgttgactc ggagtcgact tcagataaat cacgctttac
    10081 ccttgatgga gcctgtacat agatttgtgt aattgcctga ttttgatatg ttcaattcaa
    10141 catcaaatga aggttaaatt atggacgaca aacaattgca ggctcaggct gcgttcagca
    10201 aagcatcgca accggcgata gatgcttcat taaatttaag attcagcttc ctcttcagcc
    10261 acccgtacgc caatcttcaa cacttcatta tcttctttct cggccaccgt ccagatcatc
    10321 ccggcaaact ctacctggtc accgacaacc ggtgccgtgc ctaacaactg ctggacaatt
    10381 tcacccagcg tttgctgctt atcacgatat tctcgcccgt cttccaggcc atatatcagc
    10441 gcaacatcag catatttggc gctggcttcg agaatgaagt caccaaagaa gcgttgatcc
    10501 agcgcgaccg gcggcgactg gctgaacagt ttaccgagcg ccggaagatc gcgttcccga
    10561 ccaattacac acaacacatc gccttcgcgc agtcgggtgc tgccggtagg atgaagcaac
    10621 tggttatcac gaaacagtgc cgcaatacgc gtctcttttg gcatatgcaa atcgcgcagt
    10681 gccgcgccca cgcaccattt atcggcactc aattgataaa caaactgctc ccacggattt
    10741 tccggatgaa tatccaggcc aacgcgtgac accggacgtc ccactggcgg aaccaccact
    10801 ttggcttttt tagccgccca cgagagtgat gttccctgca agagcagtga aaccagaacc
    10861 acaaagaagg cgacattaaa gaacagacgt gcattctcca gccccgccat catcgggaac
    10921 acggccagga tgattggcac cgcgccgcgt aatcctaccc agctgataaa cacgcgctca
    10981 cgcagattga aaccgcggaa ggggagcaat ccagcaaata ccgaaagggg acgggcgaag
    11041 aatatcatcc atgccgacaa aattagcgcc ggaatggcaa taggcagcag atcgcttggg
    11101 ttaaccaata gccccagcac caggaacatg gcgatttgcg ccagccaggc gaggccgtcg
    11161 aaattttgca ggatgccgta gcggttgcga atcggccgat tacccagcag aaaaccgcac
    11221 agataaacag ccagaatacc gctgccttcc agcgccgtgg ttaaagcaaa aattaaaata
    11281 ccgccgctta atgccagcaa tggatataat ccggcaggca gggcgatgcg attaatcatt
    11341 tgcagcagta aataaccacc gccaagtccg atgacaatcc cgaggccaaa ttgctgcaga
    11401 atatcgacca cgaacatcca actgacactg ctttcatgtt gctggatcat cgcaatcagg
    11461 gtaatcgtca gaaagaccgc cattggatca ttactgccgg attcaatttc cagcgtcgag
    11521 ccaacacgtt cgttaagccc cttaccaccc agcagagaaa agaccgctgc agcatcggta
    11581 gagccgacga tagcgccgat taataagcct tcaatcaaat caagattaaa cagccacgcc
    11641 gccatcatgc cggttaaacc agaggtgata agcacgccca gcgtcgccag cgacagtgcc
    11701 ggtcctaacg ccacacgaaa ggaactggcc tgagtgcgca tcccgccgtc gagcaaaata
    11761 atcgccagtg ccaggttact taccatgtag gcgaaggggt aattatcaaa cgggataccg
    11821 ccgacgccat cgactcctgc cagcatgccg atcgccagaa aaataaccag aataggaatg
    11881 ccaagacggg aagaaaatga actaagtaat atactgctgg tgacaaggat ggatcctaag
    11941 atgaaaaggc taattattgt tgtggcatcc aacggtcggt tactcctgat tacgctgtct
    12001 cttatataaa ccctaccata ttagcggcag agacagcgtt ttacttagcc ctgaagcgtg
    12061 atttttttac attttaagaa caggatgacc gctaatcgtc aactgagtgc cttcccgggt
    12121 gttattcaga atggcatgtg cgcccaacgg cagcgtaacc gtgcgttgtt catgaccaaa
    12181 atcgaggccg gtaatgagtg gaatcgacag gcgggaacgc aaaaacgcgg acactgactc
    12241 caggttgtaa cctgcgtcat aatcattggg cgtgctgccg ctaaagctac cgagaataat
    12301 cgccttctga cgtggcaaaa ttcccgcatg atagagctgc aacagcatac gttcgacgcg
    12361 gaaagggtgc tcgttaatat cttccagtac caggataccg ttctcaattt ttggcatcca
    12421 cggtgtacca atcagtgaaa tcagcatcgc aagattgcct ccccacaacg tgccttcggt
    12481 ctgacatgtc gggccttccc cttgccattc aatggtgaag gttttattgc gtaacgccag
    12541 ccagaaatgg tgctcggtaa aggcgttcag ttcatccgca ccaaagtttg ctaccagcat
    12601 ggggccgcta aaggtgatga cattgccttg cgccagaaga ccgcactgaa tggcggtaaa
    12661 atcgctatgt ccgcaaatga gtaacgggtc atgttgttgg cgagccacca gtgcctgcca
    12721 gtcaatatct gccagtaaac gactggcacc gtaaccgccg cgtacagcca gcacgatggt
    12781 gttgggtgta gtcagtctgg caagggaatt gagatcttcc agacgctccg tttccgtacc
    12841 cgcaaaacgc tcacaacgac gggcaataac ctcgacgtta ttgacttgat gccccgcgtc
    12901 ggtaaggcgc tggataccac gcagcgcggc gtgctgttta atgctgtaac ccgatggggc
    12961 aattaagtga aacagagaca tggcaattcc ttgctgacaa cagaaacgaa atgtatatca
    13021 tgccgcttag gtgtgccgtt gtcacctcaa cggcgattcc aggctataag gatagaagaa
    13081 gtgaaattga gatggtttgc ctttttgatt gtgttattag cgggttgttc atcaaagcat
    13141 gactatacga acccgccgtg gaacgcgaaa gttccggtgc aacgtgcgat gcagtggatg
    13201 ccaataagcc agaaagccgg tgcagcctgg ggcgtcgatc cacaattgat cacggcgatt
    13261 atcgctatcg aatcgggtgg taatccgaac gcggtgagta aatcgaatgc cattggtttg
    13321 atgcagttaa aagcttcaac ctccggacgt gatgtttatc gccgtatggg ctggagtggt
    13381 gagccgacga ccagcgagct gaaaaatcca gagcgtaata tttcaatggg ggcggcttac
    13441 ctgaatattc tggaaaccgg cccgctggca ggcattgaag atccgaaggt actgcaatat
    13501 gcgctggtgg tgtcatacgc taacggggca ggtgcgctgc tacggacttt ctcgtcagat
    13561 cggaaaaagg cgatcagcaa aatcaacgat ttggatgctg acgagttcct cgatcacgta
    13621 gcgcgcaatc atcctgcgcc gcaggctccg cgctatatct acaaacttga gcaggcactg
    13681 gacgcgatgt aaatcagtcg cgtactttgt ccgctttctc ccgggcttcc cgctcgagag
    13741 agaaaataat ccgttgtaat tgccgctcca ccgtcgggct gacgttaaga aaacggaagc
    13801 tcagacgggg agtggtgatg gtttcattct tgccatcaat cactttgcgc tcgctgatgg
    13861 agattaactg ggcgtcaaag tgaaaaacac cccattgccc catgttgact tcaatctgag
    13921 cgaagcgcat gccttcttgt aattcggcag gctttgctgt ttccagtaat gcgcccatgc
    13981 cgcctaacga caaatcatac aggcggaaac gtaacgtact gttatccgcc agtttggtct
    14041 ggcaaaaata aggcggatgg agtggggcgg agatgcggaa atatcggcgt cgttgtacaa
    14101 accataaggt gggaggcggt acggtaataa atgccggaag ctgcaagtat tcactctgct
    14161 gtagttgttc aacagtaaac tcgactttcg caccctgagt ttcggcggta atggtaatat
    14221 gctgcgcctt tagcacggcg atgttgtctt cggcttgact gccgaaatcc agcaccagtt
    14281 tatccggggt tattgccagt attttgctga tcagctgccc gccattccaa ctgagacgca
    14341 aaggaattgc ggctttgtgc aaatcgcgta acacgcccag gacggctaac ggattttgtt
    14401 tcaggaactg ctcatggtaa tgactcacgc ggaaaaactc ctgactgaca aactgtcttt
    14461 ggtttatcgg tcacagttaa caaaacttaa tacaaatgcg tgaatatttt ttacatgttg
    14521 ttcttaaatc agccgacata cgcccagcat cgatcccgcc cttgcctata cttagagcgt
    14581 tgacgtaagc atttcttgcg tccgattcat cgaaacgagg gcttgaacat gggaattatt
    14641 gcctggatta tttttggcct gatagccggc attatcgcca agctaatcat gccggggcgt
    14701 gatggtggtg gatttttcca gacctgtatt ctcgggatag tcggtgcggt ggtcggcggc
    14761 tggctggcga ccatgtttgg cattggcggc tccatcagcg gttttaattt gcacagcttc
    14821 ctggtggcgg tggtgggagc tattctcgtt ctgggcttat tccgcctgct gcgaagagaa
    14881 taagattttc ataaggcgga tagcgataca gatgccgcta tccgcttttc acatcagaac
    14941 gtatattcga cacctgcttt acgggtgaaa aaaatcaata tagcacttcg atatccgata
    15001 aagtcggttc ccggtaacaa caacagaatc cttttttaat tattgtttcg ttgtttttgg
    15061 tgtgattcag aaaataaata ttttagacag tagccagtca ggcgttaaaa atggggcgga
    15121 tagtgaggcg gttttggctg cgcttattga acatttagaa ttgatcaatc cttctggcag
    15181 attaagccaa aatacccgtt cggcaatgct tcagttacgc gaagaggaat ggtctgaatt
    15241 ctttttttgg ttgctaaatt ctctggagtg tctggattat gtgattatta atttaactcc
    15301 agaaagcaaa aaaacgttga tgagtgaaca ccgtaacaat attcaggtag caatcgatgc
    15361 tttatatagg caacgtcggt gtaaatcacc gggggatgat agtgaaacac ttacccgtag
    15421 aaatgacgct atcttcggca accacgtatg gcaaacattt gcgcaatact tcccgccagg
    15481 acttgaaaaa ccggctgtgt agaaaacacc atagctatgg tgttatctgc cgacaacgcc
    15541 atccggagaa gccgaatgac gttgtcggag taagcgctgg ttaaggtgtg ggttgtgcct
    15601 ctttggttga gggttgcgtc gttgctgact taacggtcgg acgcgtcgcc ggaacattgt
    15661 cacacggttg ctctttcggg cagatcaaat ccagcatttt cagcgtcacg ccattggtcc
    15721 agccaaagcc atcctgtaat ggatattcgc cacctctttt tgcccgtagt tttgtaatcc
    15781 ttctgtcgcg acccactgta acggtgccca gccatttggc gcatcccatt gttgcccact
    15841 tttcaccgac gtggtgttca ggccgccggg ttgcagcaga tgtgttttcg tcgccgtcgc
    15901 cattttgttg gcgcgatctt tcgctgccgc attgacgtac agcgggaaca gggcggccgc
    15961 ggttaactga ttgcgcactt tatgactttt caggtcgtaa tcggcatacc agccttgttg
    16021 atcgttccac aggtattttt cgattccttt ttgacgggca ttcgccagcg tttcgtactg
    16081 gtttgccatc gcgttatctc ctgcagcttt gctggcgcgg gcgaggattt tttccatttt
    16141 aaacatcagg ctgttcagat cgaccggtac gatgctggtg gtgcgtaagg tatttaactg
    16201 ctgcgggttg tccatccagc gcgagctgaa atcccagcca gacgcagcgg cagagcgcag
    16261 gtcgcggtaa atttcagtgg caggtcgatt cggattgctt ttggcggtgg caatatcttc
    16321 cacccatgac tctggtcgtg gcgtatcgcg atcgtccc
//
LOCUS       DAJTOR010000085        16196 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTOR010000085 DAJTOR010000000
VERSION     DAJTOR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.085"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1996
                     /gene="crfC"
                     /locus_tag="OO249_002417"
     CDS             <1..1996
                     /gene="crfC"
                     /locus_tag="OO249_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5636684.1"
                     /translation="KAGKSTTINAIVGTEVLPNRNRPMTALPTLIRHTPGQKEPVLHF
                     SHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENGVAFEKYYLGAQPI
                     FHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGLESYPGQLTLLDTP
                     GPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREAILAVGQSVPLYVLV
                     NKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLANRARYELANNGKL
                     PAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLFAQPIQALLHAAYA
                     NASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQVEESLQLLQLNQA
                     QVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQDDSEPLSEIRTCCETLLQTAQ
                     NTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGFRPGFHFPVFHGVVPHV
                     NTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPDWGRGNEKSPTETVDYSVLQR
                     ALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQTRLRESLALRQQNE
                     SVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
     gene            1993..2876
                     /locus_tag="OO249_002418"
                     /pseudo
     CDS             1993..2876
                     /locus_tag="OO249_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
     gene            3140..4642
                     /gene="proP"
                     /locus_tag="OO249_002419"
     CDS             3140..4642
                     /gene="proP"
                     /locus_tag="OO249_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5636685.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            4708..4845
                     /gene="pmrR"
                     /locus_tag="OO249_002420"
     CDS             4708..4845
                     /gene="pmrR"
                     /locus_tag="OO249_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5636686.1"
                     /translation="MSDAHTLLQVNGGECNEKPCLYESLTTVFSVLVVSSFLYIWFAT
                     F"
     gene            complement(4821..5921)
                     /gene="pmrB"
                     /locus_tag="OO249_002421"
     CDS             complement(4821..5921)
                     /gene="pmrB"
                     /locus_tag="OO249_002421"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5636687.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5922..6590)
                     /gene="basR"
                     /locus_tag="OO249_002422"
     CDS             complement(5922..6590)
                     /gene="basR"
                     /locus_tag="OO249_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5636688.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6587..8230)
                     /gene="eptA"
                     /locus_tag="OO249_002423"
     CDS             complement(6587..8230)
                     /gene="eptA"
                     /locus_tag="OO249_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5636689.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTV
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8334..9671)
                     /gene="adiC"
                     /locus_tag="OO249_002424"
     CDS             complement(8334..9671)
                     /gene="adiC"
                     /locus_tag="OO249_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5636690.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTPGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(9808..10569)
                     /gene="adiY"
                     /locus_tag="OO249_002425"
     CDS             complement(9808..10569)
                     /gene="adiY"
                     /locus_tag="OO249_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5636691.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(10894..13161)
                     /gene="adiA"
                     /locus_tag="OO249_002426"
     CDS             complement(10894..13161)
                     /gene="adiA"
                     /locus_tag="OO249_002426"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5636692.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPSARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHAMCVKA"
     gene            complement(13360..14268)
                     /gene="melR"
                     /locus_tag="OO249_002427"
     CDS             complement(13360..14268)
                     /gene="melR"
                     /locus_tag="OO249_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5636693.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMMIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14551..15906
                     /gene="melA"
                     /locus_tag="OO249_002428"
     CDS             14551..15906
                     /gene="melA"
                     /locus_tag="OO249_002428"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5636694.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPHIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16009..>16196
                     /locus_tag="OO249_002429"
     CDS             16009..>16196
                     /locus_tag="OO249_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5636695.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMG"
BASE COUNT         3885 a         3930 c         4280 g         4101 t
ORIGIN      
        1 gaaagcaggg aaatcaacca ccattaatgc cattgttggt acggaggttc tgcctaatcg
       61 taatcgccca atgactgcgc tgccgacgct tattcgccat acgcccgggc aaaaggaacc
      121 ggtactgcat ttttcacatg tcgcgccaat cgattgttta attcaacaat tacaacagcg
      181 cctgcgtgat tgcgatatta agcatctgac cgatgtgctg gaaatagata aagatatgcg
      241 tgcgcttatg cagcggatcg aaaatggcgt cgctttcgaa aaatattatc tgggtgccca
      301 gcctattttt cattgtctga aaagtttgaa tgatttagtg cgactggcga aggcgctgga
      361 cgtcgatttt cctttttctg cttacgccgc cattgagcat attcccgtga ttgaagtgga
      421 gtttgtccat ctggcggggc tggaaagtta tcccggtcag ttgacgttac tggatacccc
      481 cgggccaaat gaagccgggc aaccgcatct gcaaaaaatg cttaaccagc agctggcacg
      541 cgcctcggcg gtactggcgg tgctggatta tacgcaactg aaatcgatct ccgatgaaga
      601 ggtccgtgag gcgattttgg cggtggggca atcggtgccg ctgtatgtgc tggtcaataa
      661 gttcgatcaa caggatcgta acagtgacga cgccgaccag gtgcgggcac tgatttccgg
      721 gacgctgatg aaaggctgta ttacgccaca gcagatattt ccggtgtcgt cgatgtgggg
      781 ctacctggcg aatcgggcgc gctatgagtt agccaacaac ggtaagttac cagcgccaga
      841 gcaacaacgc tgggtggaag attttgccca tgccgcgctc ggcaggcgct ggcgtcatgc
      901 cgatctggcg gacctcgaac atattcgtca tgctgccgat cagttgtggg aagattcgct
      961 gttcgcccag ccaattcagg cgttgcttca tgccgcttac gctaacgcct cattgtatgc
     1021 tctgcgatct gctgcgcata aactgttgaa ttacgcgcag caggcgcggg aatacctgga
     1081 ttttcgtgcg cacgggttaa acgtcgcttg tgaacaattg cggcaaaata tccatcaggt
     1141 cgaagaaagt ttgcagctat tgcaactcaa tcaggcgcag gtgagcggcg agattaaaca
     1201 tgaaatcgag ctggccctga cctccgccaa ccactttctg cgtcaacagc aagatgcgct
     1261 gaatgcgcag ttagccgcct tgtttcagga tgattcggag ccgttaagcg agattcgtac
     1321 ctgctgtgag acactgttac agacggcgca gaacaccatc agtcgcgact ttacgctgcg
     1381 ttttgccgag cttgaatcca ccctttgccg ggtgttaacc gatgttattc ggcccattga
     1441 gcaacaagtc aaaatggaat tgagcgagtc agggtttcgt cctgggtttc attttcctgt
     1501 ttttcacgga gtagttcccc acgtcaacac tcgccagctg ttcagtgaag tcatttcgcg
     1561 ccaggaggca acggacgagc agagcacgcg tttaggcgtt gtgcgtgaga ctttttcgcg
     1621 ctggttgaat cagcccgact ggggacgggg aaatgagaaa tccccgacag aaacggttga
     1681 ttacagtgtg ttgcaacgag cattaagcgc agaagtcgat ctttattgcc aacaaatggc
     1741 taaagttctg gcagagcagg tcgatgaatc tgttacggca ggcatgaata cttttttcgc
     1801 tgagttcgct tcatgtttga cggaattaca gacgcgttta cgcgaaagcc tggctctgcg
     1861 tcaacaaaat gaatcggtgg tcaggctgat gcagcagcaa ttgcagcaga ctgtgatgac
     1921 tcacggctgg atttacaccg acgcccagct gttacgcgat gatattcaaa cacttttcac
     1981 ggcagaacga tattgaccaa gacgttactg gacggccccg gtcgcgtgct ggagtcggtt
     2041 tatccccgct ttttagtgga tctggcgcag ggcgatgatg cccgccttcc acaagcccat
     2101 cagcagcagt ttcgtgaacg actgatgcag gaacttcttt cgcgtgtgca gcttcagaca
     2161 tggacgaacg gcggcatgtt aaatgcgccg cttagcctgc gactgacaat ggtggaaaaa
     2221 ctggcgtcga tgctggatcc cggtcatctg gcgctgacgc agatcgcgca gcatctggcg
     2281 ctgctgcaaa aaatggatca ccgccagcac tctgctttcc cggagctccc ccagcaaatt
     2341 gccgccttgt atgagtggtt ttcagcccgt tgtcgctgga aggaaaaggc gttaacgcaa
     2401 cgaggccagg cctactggtg caggcaggtg atcagagcga gcaaattttt acccgctggc
     2461 gtgctggggc gtataacgcc tggtcgttgc ctgggcgctg ttttatcgtt ctcgaagagt
     2521 tgcgttgggg ggcatttggc gatgcctgtc gtctgggaag cccgcaagcg gtggcgttgc
     2581 tgctgggtga tttgcgcgtg aaagcgacac aacatctggc agagagtatc aatgcggccc
     2641 cgaccacgcg tcactattac catcagtggt ttgcctcttc gaccgttctg acgggcgggg
     2701 atcatgctga ttttttaagt tggctgggaa agtggaccac ggcagataaa caacccgttt
     2761 gctggtcagt gacccaacgt tggcaaactg tcgcgctggg gatgccacga ctctgttcag
     2821 cgcagcgttt ggtgggggca atggtcgagg aaatcttctc tgtaaatttg gcgtaaataa
     2881 tcagttacat caatgagtcc taaacgaaat ccatgtgtga agttgatcac aaatttaaat
     2941 actggtaggg taaaaaggtc attaactgcc caattcaggc gtcaactggt ttgattgtac
     3001 attccttaac cggagggtgt aagcaaaccc gctacgcttg ttacagagat tgcatcctgc
     3061 aattcccgct ccccttttgc ggccgtcgcg ctgatttttc tggcgtttgc ggaaatgggc
     3121 caactctgcg aggaaagcta tgctgaaaag gaaaaaagta aaaccgatta cccttcgtga
     3181 tgtcaccatt attaatgaca gtaaactgcg taaagccatt accgcagcat cactgggtaa
     3241 tgcaatggaa tggttcgatt ttggtgttta tggttttgtt gcttacgcat taggtaaagt
     3301 ttttttcccg ggggctgacc ccagcgtgca gatggttgct gcacttgcca ctttctccgt
     3361 tccctttctg attcgaccgc ttggcggact cttctttggt atgttgggcg ataaatatgg
     3421 tcgccagaag atcctcgcta tcactattgt gattatgtcg atcagtacgt tctgtattgg
     3481 cttaataccg tcctacgaca cgattggtat ttgggcaccg attctgctgt tgatctgtaa
     3541 gatggcacaa ggtttctcgg tcggcggtga atataccggg gcgtcgatat ttgttgcgga
     3601 atactcccct gaccgtaaac gtggctttat gggcagctgg ctggacttcg gttctattgc
     3661 cgggtttgtg ctgggtgcgg gcgtggtggt gttaatttcg accattgtcg gcgaagcgaa
     3721 cttcctcgac tggggctggc gtattccgtt ctttattgct ctgccgttag ggattatcgg
     3781 gctttacctg cgccatgcgc tggaagaaac tccggcgttc cagcagcatg ttgataaact
     3841 ggaacagggc gaccgcgaag gtttgcagga tggcccgaaa gtctcgttta aagagattgc
     3901 cactaaatac tggcgcagcc tgttgacatg tattggtctg gtaattgcca ccaacgtgac
     3961 ttactacatg ttgctgacct atatgccgag ttatttgtcg cataacctgc attactccga
     4021 agaccacggg gtgctgatta ttatcgccat tatgatcggt atgctgtttg tccagccggt
     4081 gatgggcttg ctgagtgacc gttttggccg tcgtccgttt gtgctacttg gtagtgttgc
     4141 cctgtttgtg ttggcgatcc cggcgtttat tctgattaac agtaacgtca tcggcctgat
     4201 ttttgccggg ttactgatgc tggcggtgat ccttaactgc tttacgggcg ttatggcttc
     4261 taccttgcca gcgatgttcc cgacgcatat ccgttacagc gcgctggcgg cggcatttaa
     4321 tatttcggtg ctggttgccg gtctgacgcc aacactggcg gcctggctgg tcgaaagctc
     4381 gcagaatctg atgatgcctg cctattacct gatggtagtg gcggtgattg gtttaatcac
     4441 cggcgtaacc atgaaagaga cggcaaatcg tccgttgaaa ggtgcgacac cggcggcgtc
     4501 agatatacag gaagcgaagg aaattctcgt cgagcattac gataatatcg agcagaaaat
     4561 cgataatatt gaccacgaga ttgccgattt gcaggcgaaa cgtacccgcc tggtgcagca
     4621 acatccgcga attgatgaat aagctgaaac ggatggcctg atgtgacgct gtcttatcag
     4681 gccaattgaa ctcttaaggt tcacttaatc tctgacgcgc atactctcct ccaggttaac
     4741 ggaggagagt gcaatgaaaa accgtgttta tatgaaagtt taactaccgt gttcagcgtg
     4801 ctggtggtca gcagctttct ttatatctgg tttgccacgt tctgatcttt cttcagccgt
     4861 acccaggccc gcgtgccgga agtctcttgc cggttttgca ggaaaaactg cccgtgatgc
     4921 aactgtgtaa tgcggctgac aatacttaac cccagaccaa tcccgccata acggctgtcc
     4981 atacgtacaa acgctttact caactccccg catttactct catcaatacc tggtccttca
     5041 tcttcaactg ccatgaccgc tccgccatct tcttgcagct taatcataat gttgctgcct
     5101 tgcgggctgt aacgatgggc gttttctacc aggtttcgca ataacatccg cagcagggtt
     5161 gcatcaccct gaacggtgat gtcggcggcg ctctctggca atagcagggt ttgctgtcgc
     5221 tggtcgagca tggtactgag ttcgtcatac gaggggagaa tgacatcttc cagcagtttt
     5281 acatgttgat aattaccgga agaaaatgac tgtccggcac gcgccagttg cagcagctgg
     5341 gagacgctct ccatcatctg atcaagccgt gccactaacg gtgctacatc aatgtgatgc
     5401 gttttcgcca gcagttccag atgcaaacgc acccccgcca gtggcgttcg cagttcgtgc
     5461 gcgacgtcag cggtaaacaa cctttcgtta tccagcgtgc tggtcaggcg actgaccaga
     5521 tcgttcaacg ccgaaaccac cgcttcgatt tcgagggtgg cgctgtgaat ggcaatgggc
     5581 gttaagttgt cggcagtgcg cgcttccagc tctttttgca gctccgccag cgggcgggtg
     5641 atgcggcgta ccgcctgata gcagataaat agcgtcaggc tgaccataaa gacgccgggg
     5701 acaatcaggc tggcgaccgc ctcgcggatc tcacgcatga tgtggcgatc gttgttgcga
     5761 ttgtcgcgta gcgcctgctc aaacagctga atctgctcgg tactttcatg ccatagccag
     5821 aagacgctga tcagctcaaa caccaacaaa atggccccga tagtcaatat cagccgttgg
     5881 cgcagcgata ttggtcggcg cagaaaacgc atcagattca attagttttc ctcattcgcg
     5941 accagcatat agccaaagcc gcgcacggtg cggatacggg atttgcccac tttgtcgcgc
     6001 agattgtgga tatgcacttc cagggtgttg gtcgagggtt cattgtccca gttatagatg
     6061 tcgttgtaga gaatttcccg atgtaccgga ctgcctgctt tgagcattaa ccgtgacagc
     6121 agagcatatt ctttgggcgt cagaatcaac tcttcaccgc ccatccatac ctgacggcga
     6181 cccatattca gcgtcagatt gccaacaatc agctcacttt cgccctgatt attatggcgt
     6241 cgtagcaggg cgcggatacg ggcgtgtaac tcttccagcg caaaaggctt caccagatag
     6301 tcatcggcac cgacatccag cccggcgatt ttgtcggtca gcgtatcgcg agcggtgagg
     6361 atcagtaccg gcagggtata ttttttctgc cggatacggg cgagaaaatg cagtccatct
     6421 tcgtcgggta accctaaatc cagtaccacc aggctgtaat gaccggcttc aaggctttgt
     6481 tccgccatcc gcgcggttgt cacgccatcg cacgcgtagc cttcggtttg cgccgccaga
     6541 atcagtccct gcaataacag cgtatcgtct tcaacaatca gaattttcat ttactcactc
     6601 tcctgcaagt ttgcagaata tcatccgcag cctggtaata cttcgtctca acgccagtta
     6661 atcccaatag cgtggagaat aaattgtctt gtgaatagtg ttgcgtttgc gcctgttttt
     6721 gcaggcagtt ctggtcaacc tgataccgtt tttgataatc ctccgacagc cacagcagca
     6781 tcggcacctg tttttggcta tccggggcga tggcataagg cagaccgtgc agatagatgc
     6841 cattttcacc taacgattca ccgtggtcag aaagataaac caggctggtg gtaaatttat
     6901 cctgatgttc tttcagcaga ttaatcgctt tatcaacaat atagtcgacg taaaccagcg
     6961 tgttgtcgta agtgttcacc agttgctctt tggtacaggt ctggatctca ttggtgtcgc
     7021 aggttggggt aaatttcctg aactgcggcg gatagcggtt gtaataggtc ggaccgtggc
     7081 tgccgatggt gtgtaagaca atcacgccat caccctgcag gttattgatg tactcttcca
     7141 gcccgtggaa cagcacttcg tcatagcatt cgccgttgat gcactgacca ggcagattca
     7201 gcgcggtgac gttctggtga ggcacgcggt cgcaggcacc tttacagccg ccatcgttgt
     7261 cattccacag cacgttgatg cccgctcgct gaatgatatc cagcacgcct tcctggtgct
     7321 gtgccagctc ttctttgtaa tgctcacgcg gcatatccga gaacatgcac ggtactgaaa
     7381 ctgccgttgc cgtgccgcaa gatacggtat tagggaaata gaccacgtta tctttcgcca
     7441 gccgcgggtt agtttcacgc gggtagccgt tgagggagaa gttctccgcc cgcgaggttt
     7501 cgccgacaat caggatggtc aaatttttac gtttttcgtt ctgcattaac gggttgcggt
     7561 gggcgtcttc accaattcgc accagcggta gatttgccag tcgctgatgg gagtaccatg
     7621 accagctggc aacaatgctg ttagaggggc ttaaggattt caccagctct ttgttattgc
     7681 ggaacaacga ggcgtagtct ttataaaaca gtgcggcgac cagcaaaatc agtagtacag
     7741 aaaccagaat attggctcca cggaaaagaa cactgcgcag acgcgaggtg gcaggtttga
     7801 tttttatcca gcaggcaatc agcgcagcaa gcacgccgct gaatcccagc gttaataaca
     7861 tttgcggtgt catcagcgca taactttctg ccggagtggt atcaataata ttggcaatca
     7921 tcgagcggtc gatgacgatg ccgtaagtca ttatgaaata ttgtgcagcc gcgccaacca
     7981 gaataaacag gcaggccagt ggtcgattaa gccataagaa agagcttagt gtcaggacaa
     8041 tattaatcac gctgaaagcg acgaccggca tcgacaagaa aaccagtacg ttatgcagcg
     8101 aatccagcgg cagcgcctgc aacacctgtt taaaaaaggc aatattcagg cagatagaga
     8161 tataaaaagc ggccaacaat agccaggcga gtaaattcaa agagggtctt tttagtaggc
     8221 gcttcaacat cacggtgttt ccatcgaaca aagtgcgcat atgctcgcaa agcaaaatta
     8281 agccaacctt aagttcttaa ggttggcttt tatgtttgct ggattaaggg gaattaatct
     8341 ttgcttattg gtgcatctaa gggatacggg tttttatgta gccggttgta attcagcgca
     8401 tacatggcgg tgatgaccat cagggtgaca aatgaccaca taacctcttt cgctccggac
     8461 cccaccacgg cccagatgca gtagaggaag gcaatggtag taactgccag atatgccggg
     8521 cgtgctttac caaagtgacc gtgtccgagc agcagtaacg ccgcacaggt gtaaagatat
     8581 ggcaccagtg taaagatgac cgagacggaa gaaaccagac cgaactcttt ggtcgcgttt
     8641 ggtgaaatgc tgctgagctg gaagatggtc atcaaaatac cgacgataat cagccccgcc
     8701 actggcgtac ccgctttatt tacacgggca aaaatcggtg ggaacagtcc gtcatcggca
     8761 gcggctttcg ccgtttgacc cgccagcaac gtccagccgc ccagtgaacc taagcaaccc
     8821 gcagctgcgc agaaggaaac aatggccccg ggggtgtcac ccaacgccat ccgtgcggca
     8881 tcaccgaatg gcgaagcaga aacgcgcagt gcggcattag ggatcatccc cataatcgcg
     8941 gtggtagaaa gtacatagca aacggcggca atcaataccc caccaatggt ggcgataggg
     9001 acattgcgtt tcgggttttt caccacacct gcggcaacgg aggcactttc cacaccgatg
     9061 aacgaccaca gcgtaacgtt aagggtactt tgaattgcac cgaaggtacc caggccgctg
     9121 acgttccatg ccgccatata ggtttcacca cggaaccaga accagccaaa tacggcaatc
     9181 ccgacgatgg ggatcagcgc cagcacggtg gcaactgcct gcacacgggt gatcattttc
     9241 ggaccaacaa tgttcagcag gacgaagatc cacagcacca cgacgcaggt gatggttaat
     9301 accaatggat ctttcagaat cgggaagaag taacttaaat atcctacgcc aatgaccacc
     9361 atggcgatat tgccgatcca gcaggccagc cagtagagga cgttggtttg ataaccgaga
     9421 aacgggccaa agcagcggcg ggcgtaagcg taagaaccac caggacttgg gtcgaggaac
     9481 gacattttgg cgtataccat cgagagcccc agtgcaccga taatcgtcac caaccatcca
     9541 taaatggcaa tcccgccagt ggaggccagg tttgcaggta acagaaaaac acctgacccc
     9601 ataatattcc ccgacaccat cagggtgacg gggattaagc ccactttgtg agcatcagca
     9661 tccgaagaca taattaaact cctgcgaagg cgagcttcgt gacaataaat tacgtcatat
     9721 catacgcctg cattcgatag ttgaattatt atcgaccggg ttatcgctga tgccgttatt
     9781 aatatagctc ttcgttaagg ttaaaaatca ggcgacagtg cgttctctgt gctgactaac
     9841 ataatgcagc ggcgtcatac cgtagaagtc tttaaagaca gaaataaagt acgacgtact
     9901 gttgtagccg caggactgtg atacctgaga gatattttta ccgtccatca ttaattcatt
     9961 tacggcataa cgcatgcggc aggtggtgat tatttggcta taactggtgt tttcgctttt
    10021 cagctttttc tttaacagac ttgggctaag acataaacaa ctggctacca tactaagatt
    10081 ccagtctttg tgaatatcgc tttcaataat ttgataaacg ctgtcactta cacaattacg
    10141 taacatatac atcattaatg aaaggaattt tttactgtcg agaaaacggg atagcaccgt
    10201 gaataataat gcgcgtgttc gttccttttc tgactcagtt tctgcgggca tcatgctgtg
    10261 ttgcgcggca acgcgaaaga cgtctggcgt caggcatggc agggtgagta tgggcgtagc
    10321 gctacgttgc catactggta tttgcgagag atctttattc agaaaccgga ggtaatcttt
    10381 gatgatgtcg tgactaatat gagcaaccaa agtgttgtta agagaggaga cgtcgataat
    10441 attattttca caatttaata acgccatatg gttagcttta aggctaatag gttctttccc
    10501 attcaccctt atccagacat ctttttcagt caataaaaca atacaaggtt ggtcgctgca
    10561 aatcctcata catgtactcc tgagtgcgaa taaaagacca gttctcgcta aagcaaagcg
    10621 ataccgagaa atggttgtat aattgtatga cgaatataaa catgcataaa aataacgagt
    10681 tttactaata ttacaaattt ttatagtaat ttctcatgta catatttttt tacagggtaa
    10741 ataacaggca aaaaaaagcg cggggtgggg ccgcgcaaaa agtatgacat ttttgtttgc
    10801 agcaattttt atgaaaacgt tgccatactc aaaaggccta aggccaggcg gcgtaatgtt
    10861 atttaaacaa ttacgccttc agcggaatag tggttacgct ttcacacaca tagcgtggta
    10921 aataccgtca ataatttcag tcccttcagt ttcgtgttca aatccaggga aatggtggtc
    10981 ccaggattgc agcgagcgta aataacttac ttgcggactg tttttatcgc cgaagttttc
    11041 accagacagc agcatcggga ttcctggcgg atatgggata actgagtttg ccgcgatgcg
    11101 tcctggcaga ttttcaatgg ataccagttc gacattgttg tcgacaatcg cgttgtacgc
    11161 ttcacgcggg gtgatttccg ccaccggcag gccggaatag gcttcgttca accgtgcgct
    11221 agggttgttt tctttcagcc aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt
    11281 cgcgtaagtg tcaggatatt gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt
    11341 ggcgtcatag tggcgtttga aggagcaaag ggtgttaacc agagtccccc atttcccacg
    11401 ggttacgccc atagagaaca ggaacataat ttggaagtca gtggtgcggg taggcacaat
    11461 gccgtggcga ccaagccagg cagtgaccag cgccgccgga acaccggttt cttccagttc
    11521 accatcttca cccattcccg gagcaaggat gctgacttta atcgggtcga gcatactcca
    11581 gttatccgga atatctttga agccgtgcca gctttcgccc ggatgcatta cccagtagtc
    11641 ctgaacggtg gtcagcagtt tggttggtgc gtcagcaaag tcataggttt tgccggtttg
    11701 tgggtcggtg acgacttctt tgttccacgg tttgaagaac cagctaccgt cagcggtgaa
    11761 ctctttatac agccgcgcca tcgcctgacg gaaatcaacc gcttcgtcga tcacttcctg
    11821 cgtcagtgac aggccgctgt tgccgtccat catcgacacc gccacgtcgt tggatgcgca
    11881 gatggcatac agcggggagg tggtggcatg catcatgtag gcctggttga agcgggagaa
    11941 gttaatcgcc ccacgacctt cacgtacatg aatataagaa gcctgtgaaa gcgcattcag
    12001 cagtttgtgg gtggagtggg tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc
    12061 gcgcatggca tagtgatcgg catagatcgg gttgaaacgt gcatagccgt accaggcttc
    12121 gtcaaagtgc agacgatcgg aggttttttc cagcagatcc tgcgcttctt tagcgttata
    12181 acacacgccg tcataggtgc agttggtcac cacgcagtaa gacggttttt gcccggcttt
    12241 gtctttagtc agcgggcttt cactgatttt cttctgcaag gtttcaggtt gcatttcctg
    12301 cggatagatt ggcccgataa tgccgtagcg gttgcggctt ggcaccatgt agaccggttt
    12361 cgcgcctgtc agcatcaagc cttgttcgat ggatttatgg cagttacggt caacgaccac
    12421 gacatcgtta tcggtcatgc aagcctgcat gatggtgcgg ttagagccgg aagtaccgac
    12481 gactaccgac caggagcgat cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc
    12541 aaatgcgcca gtatggtcaa gcaaagaacc gagggaagtt cgttcgatgc ccatatcggt
    12601 gcggaacaga ttttctccat agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa
    12661 accaacgccg ccctggtggc ctggcgctgc ccaggaatat tcatggatgt cgctatactt
    12721 catcagcgcg ctgaacagtg gcggcaacag ctgctggcgg tagcgggtca tcgcggcaac
    12781 ggcgcgtccg gcgataaagt cggtggtatc ttccagaatc caggcgaatt catcgacaag
    12841 ctccagcagg tcgcgatcca ttgcggcgag ggctttttcc cgatcgccca acaggaagac
    12901 cggcacgttt tgttggcgct catgaagctt accgatcaat tgtctgacgt tttgatgttc
    12961 gtccggatgt tccatttgat agctgaacat caggcagtca atggcttcgt ttgaagagag
    13021 aatggcaaaa ccatcatcaa aggaggtgga tttaatcacg gtaacatttt gctggcttaa
    13081 agcatctgcc agacgctcaa cggcgttacc gacccaggtg tcttgatgga gaaactcgct
    13141 ttcaacaatt aatactttca tcattgctta cccggttatg aaggaatctt cgttgcgggc
    13201 aagtatcttc cggccttttc gggctgtaaa gcgcgtgaaa aacaaacatt tttgaaatat
    13261 ttatttttat aaacaatgcg ttatgtgatt attttaaatt attttcactg cttgttatcg
    13321 acggctaaac tatttttttg gctgatactg atatcgtctt tagccgggaa acgtctggcg
    13381 gcgctgttgg ctaagtttgc ggtattgttg cggcgacatg ccgacatatt tgccgaacgt
    13441 gctgtaaaaa cgactacttg aacgaaagcc tgccgtcagg gcaatatcga gaatactttt
    13501 atcggtatcg ctcagtaacg cgcgaacgtg gttgatgcgc atcgcggtaa tgtactgttt
    13561 catcgtcaat tgcatgaccc gctggaatat ccccattgca tagttggcgt taagtttgac
    13621 gtgctcagcc acatcgttga tggtcagcgc ctgatcatag ttttcggcaa taaagcccag
    13681 catctggcta acataaaatt gcgcatggcg cgagacgctg tttttgtgtg tgcgcgaggt
    13741 tttattgacc agaatcggtt cccagccaga gaggctaaat cgcttgagca tcaggccaat
    13801 ttcatcaatg gcgagctggc gaatttgctc gttcggactg tttaattcct gctgccagcg
    13861 gcgcacttca aacgggctaa gttgctgtgt ggccagtgat ttgatcatca tgccgtgagt
    13921 gacgtggtta atcaggtctt tatccagcgg ccaggagaga aacagatgca tcggcagatt
    13981 aaaaatcgcc atgctctgac aggttccggt atctgttagt tggtgcggtg tacaggccca
    14041 gaacagcgtg atatgaccct gattgatatt cactttttca ttgttaatca ggtattccac
    14101 atcgccatcg aaaggcacat tcacttcgac ctgaccatgc cagtggctgg tgggcatgat
    14161 atgcggtgcg cgaaactcaa tctccatccg ctggtattcc gaatacagcg acagcgggct
    14221 gcgggtctgt ttttcgtcgc tgctgcacat aaacgtatct gtattcatgg atggctctct
    14281 ttcctggaat atcagaatta tggcaggagt gagggaggat gactgcgagt gggagcacgg
    14341 ttttcaccct cttcccagag aggcgagggg agcgtccgag catcatgagg ccgaaaactc
    14401 tgcttttcag gtaacttatt accataaact cagatttact gctgcttcac gcaggatctg
    14461 agtttatggg aatgctcaac ctggaagccg gaggttttct gcagattcgc ctgccatgat
    14521 gaagttattc aagcaagcca ggagatctgc atgatgactg cacccaaaat tacatttatc
    14581 ggcgctggtt cgacgatttt cgttaaaaat attcttggtg atgtgttcca tcgagaggcg
    14641 ctgaaaacgg cgcatattgc cctgatggac attgatccca cccgcctgga agagtcgcat
    14701 attgtggtgc gtaagctgat ggattcagca ggggccagcg gcaaaatcac ctgccacacc
    14761 caacagaaag aagccttaca ggatgccgat tttgtcgtgg tggcatttca gattggcggt
    14821 tatgaacctt gcacggtgac tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc
    14881 attgccgata cgttggggcc gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg
    14941 tggcaaattt gcgaggacat gacggaagtc tgccccgatg ccacattgct caactacgtt
    15001 aacccaatgg cgatgaatac ctgggcgatg tatgcccgct atccgcatat caaacaggta
    15061 gggctgtgcc attcggtgca gggaacggcg gaagagctgg cgcgtgacct caatatcgat
    15121 ccgtccacgc tgcgttaccg ttgcgctggt atcaaccata tggcgtttta cctggaactg
    15181 gagcgtaaaa ccgccgacgg cagttacgtg aatctctacc cggaactgct ggcggcttat
    15241 gaagcagggc aggcaccgaa gccacatatt catggtaata ctcgctgcca gaatattgtg
    15301 cgctacgaaa tgttcaaaaa gctgggctac ttcgtcacgg aatcgtcaga acattttgcc
    15361 gaatatacgc cgtggtttat taagccaggt cgtgaggatt tgattgagcg ttataaagta
    15421 ccgctggatg agtacccgaa acgctgcgtc gagcagctgg cgaactggca taaagagctg
    15481 gaggagtata aaaacgcctc ccggattgat attaaaccgt cacgggaata tgccagcaca
    15541 atcatgaacg ctatctggac tggcgagtcg agtgtgattt acggcaacgt ccgtaacgat
    15601 ggtttgattg ataacctgcc acaaggatgt tgcgtggaag tagcctgtct ggttgatgct
    15661 aatggcattc agccgaccaa agtcggtacg ctaccttcgc atctggccgc cctgatgcaa
    15721 accaacatca acgtacagac gctgctgacc gaagctattc ttacggaaaa tcgcgaccgt
    15781 gtttaccacg ccgcgatgat ggatccgcat actgccgccg tgctgggcat tgacgaaata
    15841 tatgctcttg ttgacgacct gattgccgcc cacggcgact ggctgccagg ctggttgcac
    15901 cgttaaaacg cgactaaacg ctactgcgcc gggggattta ttccggcgca cacctctgac
    15961 gataccaata acagaaggcg ggcgttggta acagcgaccc gataccctat gagcatttca
    16021 atgactacaa aactcagtta tggatttgga gcgttcggga aggattttgc gatcggcatt
    16081 gtgtatatgt acctcatgta ttactacacc gatgtcgttg ggctgtctgt gggtttggtc
    16141 ggtactttgt ttctggtggc gaggatctgg gatgctatta acgatccgat tatggg
//
LOCUS       DAJTOR010000086        15695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTOR010000086 DAJTOR010000000
VERSION     DAJTOR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.086"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            131..379
                     /locus_tag="OO249_002430"
     CDS             131..379
                     /locus_tag="OO249_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5636696.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            357..1487
                     /locus_tag="OO249_002431"
     CDS             357..1487
                     /locus_tag="OO249_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5636697.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRRLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1533..2171)
                     /gene="ompW"
                     /locus_tag="OO249_002432"
     CDS             complement(1533..2171)
                     /gene="ompW"
                     /locus_tag="OO249_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5636698.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2411..2500
                     /locus_tag="OO249_002433"
                     /pseudo
     CDS             2411..2500
                     /locus_tag="OO249_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2528..3271
                     /locus_tag="OO249_002434"
     CDS             2528..3271
                     /locus_tag="OO249_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5636699.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            3301..3840
                     /locus_tag="OO249_002435"
     CDS             3301..3840
                     /locus_tag="OO249_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5636700.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3945..4343
                     /gene="yciA"
                     /locus_tag="OO249_002436"
     CDS             3945..4343
                     /gene="yciA"
                     /locus_tag="OO249_002436"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5636701.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4383..5111)
                     /gene="tonB"
                     /locus_tag="OO249_002437"
     CDS             complement(4383..5111)
                     /gene="tonB"
                     /locus_tag="OO249_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5636702.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTALARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            5326..5622
                     /locus_tag="OO249_002438"
     CDS             5326..5622
                     /locus_tag="OO249_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5636703.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5922..7175
                     /gene="kch"
                     /locus_tag="OO249_002439"
     CDS             5922..7175
                     /gene="kch"
                     /locus_tag="OO249_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5636704.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7230..7403)
                     /locus_tag="OO249_002440"
     CDS             complement(7230..7403)
                     /locus_tag="OO249_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5636705.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7546..9006
                     /gene="cls"
                     /locus_tag="OO249_002441"
     CDS             7546..9006
                     /gene="cls"
                     /locus_tag="OO249_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5636706.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9041..9370
                     /locus_tag="OO249_002442"
     CDS             9041..9370
                     /locus_tag="OO249_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5636707.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9423..10427)
                     /gene="oppF"
                     /locus_tag="OO249_002443"
     CDS             complement(9423..10427)
                     /gene="oppF"
                     /locus_tag="OO249_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5636708.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10424..11437)
                     /gene="oppD"
                     /locus_tag="OO249_002444"
     CDS             complement(10424..11437)
                     /gene="oppD"
                     /locus_tag="OO249_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5636709.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTTVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLCACFKPVE
                     ELL"
     gene            complement(11449..12357)
                     /gene="oppC"
                     /locus_tag="OO249_002445"
     CDS             complement(11449..12357)
                     /gene="oppC"
                     /locus_tag="OO249_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5636710.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12372..13292)
                     /gene="oppB"
                     /locus_tag="OO249_002446"
     CDS             complement(12372..13292)
                     /gene="oppB"
                     /locus_tag="OO249_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5636711.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13378..15009)
                     /gene="oppA"
                     /locus_tag="OO249_002447"
     CDS             complement(13378..15009)
                     /gene="oppA"
                     /locus_tag="OO249_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5636712.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            15307..15603
                     /locus_tag="OO249_002448"
     CDS             15307..15603
                     /locus_tag="OO249_002448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636713.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSFSIEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
BASE COUNT         4039 a         3799 c         3683 g         4174 t
ORIGIN      
        1 cgttaccagg caaaatacat ttcagttatc tggcaaaaac tgatcccgaa tcaggctgat
       61 ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc atgattagcg
      121 aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg aattcagtga
      181 accggtccct actccgagta cgttaagtaa atacgctaaa gccggaatga tatttcctct
      241 ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg tcggaatggt
      301 aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac tggaagatgg
      361 cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt gacaaaagaa
      421 ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac ggtctcggca
      481 gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt cttgcccggc
      541 agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc ggcggttcat
      601 caactgttac catttttctg aataattaca gaaaaattca acaggaaaga tatgaaaacg
      661 gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta tttgatgaac
      721 gttttggcac cagacggtta gatgccataa ccgtaaaaga tgtggtatca gtactggaag
      781 agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta ctgatcgatg
      841 ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct gcagaatcgg
      901 caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag tggatgatga
      961 tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg ctggcactga
     1021 tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc cgggatggtt
     1081 atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg gctctgcgtt
     1141 gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat tgcgttctta
     1201 gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc gggatggtta
     1261 agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat tacaactggc
     1321 gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag cgattgttca
     1381 gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa atgaccgata
     1441 tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga ccagttttgc
     1501 agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc ggagaacata
     1561 aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga tttgtactta
     1621 gccgtggtat cgatatccat gtaccagact gacatgttaa ccaaccagtc acggttaatc
     1681 agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact gagatcggaa
     1741 agccctgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt gtagttaata
     1801 cctgccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca ctgcgccatc
     1861 agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt gccgatttta
     1921 tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc catataagta
     1981 aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact tcctaacgta
     2041 ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa aaattcgcct
     2101 gcttcatgcg caaaggcact gccagagaga agagttgtta ctgccaaagc cgccactgtt
     2161 aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata tacctatatt
     2221 ggagtaacaa atgatcctac atagatcaca ttacatatga taattcaccc tttattgatc
     2281 tggattaatt tagaagttgc attgagaatc ctggttaacg tgatttaaat caatttttaa
     2341 atattttcgc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc ccacgcctga
     2401 aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac ctgatgctgc
     2461 cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata aaactcaagg
     2521 agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt tcttccgtaa
     2581 tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag tggtgttagg
     2641 gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg tgcccgttag
     2701 cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc aacaaatttt
     2761 gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca ttctcgccgg
     2821 aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg cgctgcgagc
     2881 tattggtgcc agtgcaccga tattgccaaa gttatttatt ctgatttttc tgactaccct
     2941 tttagtacag attggcatca tgctagtggt cgttccggga attatcatgg ccattttact
     3001 ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct cgatgcgtag
     3061 cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac tgagctggtt
     3121 gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa ccccggaaat
     3181 tggtgccgta ctggcgaaca ccttgagcaa cctgatttca gccgtattgc tcatctatct
     3241 gttccgtctg tatatgttga ttcgccaata accttaagca tttgattacg gaatcgtaaa
     3301 atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta caagatttat
     3361 gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct tatctatagc
     3421 tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct ggtggtcgtc
     3481 ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa ggttacagtc
     3541 atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa aaagccgcta
     3601 attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc gaagctgaat
     3661 ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat cgcattctgg
     3721 ctgccgcaaa atatttgggt caactttaaa gtctttggac tgaccgccct taccttaatc
     3781 ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga taaatcctaa
     3841 ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc aggaaaatca
     3901 tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct acaacacata
     3961 acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat accaatgcca
     4021 atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc gctattctgg
     4081 cgaaagaaat tgcccacggt cgcgtagtga ccgtgcgggt tgaaggaatg actttcttac
     4141 ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag aaagggacga
     4201 catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa ccaatcgggc
     4261 aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct gaaggaaaac
     4321 ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt ttgactttct
     4381 gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac aatcccactg
     4441 cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc acgctcaaac
     4501 atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc cggcgtgacg
     4561 tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc cggatactgc
     4621 ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg cttgctggtt
     4681 gcagccgttg ctgtacttga tgtcaggcgt gccagtgccg tattttcaaa cggtgatgcc
     4741 ggacgcgact ctacgggttt gacatcgcgt tttggctgct cctgtacctt tttcaccggc
     4801 ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc ttcttttggc
     4861 ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg tggcggctga
     4921 acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat tggctgcgca
     4981 ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc cacaacagca
     5041 ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa atcaagggtc
     5101 attgaagtca taatcatttc agtagaaaaa ccaagcctcg attttaaatg caaatagcaa
     5161 tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg ccgtaattaa
     5221 ccttacttta tgcatggtct taacaaagac atcatctttc cgttgcagat cttcgccctt
     5281 tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt gtatgttatt
     5341 tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc ggcacattta
     5401 gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc aatgccagcg
     5461 gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat tgctgaattt
     5521 gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc ggcaggagtc
     5581 tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat tcaatattta
     5641 agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg gtcagtgcat
     5701 tcaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt gagcatattc
     5761 aggaaaaatg acctttcctg aggtacagtt gctcgctcaa aacctttgat tactgcggat
     5821 aaagtcatct actattgttt aacacattca cgcgctgcat gtatgttcag aaaatgtgca
     5881 cgctaagata attaaacaaa attgtggtta cagaggtatc agtgagtcac tgggctacat
     5941 tcaaacaaac agcaacaaat ttatgggtga ccctacggca cgacattctc gcgctggccg
     6001 tctttttaaa tggattgctt atttttaaaa caatctatgg tatgtcggtc aatttgctag
     6061 atattttcca tatcaaagca ttttcagagt tggatctttc cttgctggca aacgccccac
     6121 tatttatgct cggcgtcttt cttgtcctga actccattgg cttactgttc cgggcaaagc
     6181 tcgcatgggc aatcagtatc attttgttgt tgatagcgct aatttatacc ctgcattttt
     6241 atccctggtt gaaatttagt attggatttt gcatttttac gctggtgttt ttgctgatac
     6301 tgcgcaaaga cttctcccac agtagcgccg cagccgggac aatttttgca tttattagtt
     6361 tcacgacatt actgttttac tcaacctacg gtgcgcttta tttaagcgaa ggttttaatc
     6421 cgcgaataga aagtttgatg accgcgttct atttttcgat agaaaccatg tcaaccgtcg
     6481 gctatggcga tattgtccct gtttctgaat cagcacgatt gttcactatt tcggtcatta
     6541 tttccggtat taccgttttt gccacatcta tgacctcaat ttttggtccg cttatccgcg
     6601 ggggattcaa caaacttgta aaaggaaaca atcatacaat gcatcgtaaa gatcatttta
     6661 ttgtttgcgg acattcgatt ctcgccatca atacgattct gcaactgaat caacgcggac
     6721 aaaacgtaac ggttatcagc aacttgcctg aagatgatat caagcaactt gagcaacgct
     6781 taggcgataa cgctgatgtt atccccggtg acagtaatga cagttcagta ttaaagaaag
     6841 cgggaatcga tcgttgcctg gccattctgg cgctgagtga taacgatgca gataacgcgt
     6901 ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa aacagttctc gccgtcagtg
     6961 atagcaaaaa cctgaataag attaagatgg tacatccgga tatcattctc tcaccgcaac
     7021 tgtttggcag cgaaattctg gcgcgagttt taaatggtga agagattaat aatgatatgc
     7081 tcgtttcaat gttgttgaac tccggtcatg gcattttcag cgataacgat gaacaagaaa
     7141 cgaaagctga cagtaaagaa tcggcgcaaa aatagagttt aaagatggag gagtaaactt
     7201 attgggcacc gcaatggtgc ccagtacatt tagatattgt agatcgcaaa caataacgag
     7261 ttacgctgtt tgtttagaat gcacttcctg atgctgtgga tacgcatatt tcggcgcgat
     7321 tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac gctgcatgcg ccagcgcccc
     7381 acttccgttc tactccgctt catgttaact actctatgca ataacagaat ggtcattata
     7441 cccccgcccc cgacgctgac cagtgctttt cccgcgtttt tatttgccag atgaatccat
     7501 atgcgtaaac tcataacaat gcgctttcaa aaggatttct aatctatgac aaccgtttat
     7561 acgttggtga gttggttggc cattctggga tactggttgc tcattgcagg cgtaacttta
     7621 cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg cctggctgtt gattatttac
     7681 attctgccgt tagtcggaat tattgcctat cttgccgttg gcgagctcca tttaggcaaa
     7741 cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg caaaatggct taacgacctt
     7801 aaagcctgta agcatatctt cgccgaagaa aatagcagtg tcgctgcgcc attattcaag
     7861 ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga atcagctaca actgatgacc
     7921 gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc agctcgcgcg ccataatatt
     7981 gagattgtgt tttatatctg gcagcccggc ggcatggcgg accaggtggc tgaatcatta
     8041 atggccgctg cgcgacgcgg cattcattgc cgattgatgc tcgactccgc cgggagtgtg
     8101 gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg ccggtattga agtggtcgaa
     8161 gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta tggacctgcg ccaacatcgc
     8221 aagatgatca tgatcgataa ttacatcgcg tacaccggca gcatgaatat ggtcgatcct
     8281 cgctacttca aacaagatgc gggcgtaggg caatggattg atctgatggc gcgtatggaa
     8341 ggccccatcg ccaccgcgat ggggattatt tattcctgcg actgggagat tgaaaccgga
     8401 aaacgtattc tgccgccacc accagatgtc aatattatgc cgtttgaaca ggccagcggt
     8461 cacaccattc acacaattgc ttctggcccc ggctttccgg aagatctcat tcaccaggca
     8521 ttattgactg cggcttattc ggcgcgtgaa tatttgatca tgaccacgcc ctactttgtg
     8581 ccaagcgatg atttacttca tgcgatttgc acggcggcac agcgcggggt ggatgtcagt
     8641 attatccttc cgcgaaaaaa tgactcgatg ctggtcggct gggccagtcg cgcattcttt
     8701 acggaacttc ttgctgctgg ggttaaaatt tatcagtttg aaggcgggtt actgcatacc
     8761 aagagcgtgc tggtcgatgg cgaactaagt ctggttggca ccgttaacct tgatatgcgt
     8821 agtctgtggc tgaatttcga gattaccctg gcaatcgacg ataaaggttt tggtgcagac
     8881 cttgccgccg ttcaggacga ttatatttcg cgttcacgtc tgctcgatgc ccgtttatgg
     8941 ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt tttacttctt cagcccgttg
     9001 ctgtaaaacg tctccaacag aggttaaaca ggtagtgact atggatatgg atctaaacaa
     9061 tcgcctgact gaagatgaaa cgcttgagca ggcctacgat atttttctcg aactggctgc
     9121 cgacaatctc gacccagctg atgttctgct gttcaatctt cagtttgaag agcgcggcgg
     9181 cgcggagtta ttcgatccgg tggaagactg gcaggaacat gttgatttcg atttgaaccc
     9241 ggactttttt gccgaagtgg tgattggtct ggcggacagt gaggatggtg aaatcaacga
     9301 tgttttcgcc cgcattctgt tatgccgcga aaaagatcac aaactttgcc atatcatctg
     9361 gcgggaataa ataatcgctt tcggcgtgaa aaagggctga caactgtcag cccttattgt
     9421 gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc tgaaactccc ttccagcaca
     9481 ggacgtgttt tggcgcactc cggaccggca atcgggcaac gggtacggaa aacacaaccg
     9541 gaaggcgggt tgatcggcga cggtaattcc ccttccagta actggatggt tttgttcttc
     9601 tccagatccg gatcaggtat ggggactgcc gacatcaatg ccctggtgta aggatgtagt
     9661 ggattgtggt agacctcatc ataggtcccc agttctaccg catggccgag atacatcacc
     9721 aacacacgat cggaaatgtg ttttaccacg gccaggtcat gagcaataaa aattaatgac
     9781 aatcccatct cacgttgcag ctgctggagc aggttgacca cctgcgcctg aattgacacg
     9841 tccagcgccg acaccggctc atcacagata atcagcttcg gttcaagaat aagagcacga
     9901 gcaatcccga tacgctggca ctgcccacca gagaactcat gcggatagcg gttaatcagg
     9961 ttaggcaata acccgacttt cagcatcatc gccttcacgc gctcgcgaac ttcctgacgt
    10021 gacattttcg gatgataagt acgcagtggt tcagcgatga tctcgccgat ggtcatgcgc
    10081 gggtttagcg atgccaacgg atcctggaaa atcatctgaa tatcactgcg aacggcacgc
    10141 cattcatcgg gcttaatgcc cagcaactct ttacctaacc aggcaacatg accgtcggtc
    10201 gctttaacca ggccaatgat ggcgcgagca aaggtggact taccgcatcc cgattcccct
    10261 accacaccta atgtttcccc ttcatacagg cgaagcgtga caccatcgac ggctttgagc
    10321 gttttcggcg gttgccagaa ccactgtttg ccatctttga tttcaaagtg cactttcaga
    10381 tcggcgattt caaggaggac ttttcttcct tcggttacag cattcataac agttcctcca
    10441 ccggtttaaa gcaagcacac agacggccaa gcgtaaactc ttccagcggc ggtgcgctac
    10501 tacaaatttc catcgcatgc ggacaacgtg gctggaacgg gcaacctttc ggtaatcgca
    10561 gcaggtttgg cggattaccc gggatggtca acattgtttc accttccgca tcgagacgcg
    10621 gcaccgcgtt gagcaaaccg atagaataag gatgaacggg ttgataaaag acatcgcgcg
    10681 cgttgccata ttccatcgtg cgcccggcgt acattaccag cactttgtca cagatccccg
    10741 ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt attaaattcc cgtttcagtt
    10801 catttaatag cgtcatgatc tgcgcctgta cggtgacgtc cagcgctgta gtgggttcat
    10861 ccgcaatcag cagcttaggt cgacatagca atgccatcgc aatcataact cgctgacgca
    10921 tgccgccaga aaactcgtgc gggtacattt tcatgcgttt acgtgcttcc ggcattttta
    10981 ccgcatcgag catccgcacc gactcttcaa acgcctcagc tttgctcatg ttcttatgca
    11041 gcatcagcac ttccatcaac tgctcaccga cgcgcatata gggattcaac gaagtcattg
    11101 ggtcctgaaa aatcattgag atttgttcag cgcgcagttt attgagttca tgctctggca
    11161 aattgaggat ttcacgccca ttgaaggttg ccgatccgcc aatacgtccg ttggcagcca
    11221 acaggcccat caacgcaaat gcagtttgcg atttacccga accagactca cctacaatac
    11281 ccagcgtttc tccggcacgt agggaaaaat tcaaatcatt gaccgtcgtg acgtcgccgt
    11341 ccggggtact aaaggtgaca cgcaaatctt tcacgttcag cagtgcgtca gcctgttgtt
    11401 gtgcgagcgg cacagttgca gtttcaatta cgctcatggc tgcactcctt aacgatcttt
    11461 cgggtcgagg gcatcacgca agccatcgcc gataaagttg aaacaaaaca gcgtcaccac
    11521 gaggaatccc gctgggaaca acaataacca tggagagact tccatcgagt tcgcgccatc
    11581 actcagcaat gcaccccagc tgcttaacgg ctcttgcgta cccaacccca ggaagctaag
    11641 gaaagattca aagaggatca tgctgggcac cagaagtgat gcgtagacca ccaccacacc
    11701 gagtacattc ggcacaatgt ggcgaataac aatgcccgat gtcgatacac cgccaacttg
    11761 tgccgcctca ataaactctt tgcgcttcag actcagggtt tgcccacgca caatacgagc
    11821 catatccagc caggaaacca tgcctatcgc cacgaaaatc agcaggatgt tttgaccgaa
    11881 aaaggtcacc agcaaaatga cgaagaacat gaatgggaag gagttgagga tttccagcag
    11941 acgcatcatt accgaatcca ctttaccgcc cagataaccg gaaagcgaac cataaagtgt
    12001 cccgacgacc acagccacca gtgccgcagc aacaccgacc atgagtgaga tacgcccacc
    12061 aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca gtaccaaagt agtgaccgga
    12121 ctccatatcc ggggcgctgg acatcatcgc ccagtcagta tcgtcatagg caaactggga
    12181 aagcatcggt gccaggatta caaataacgc gatcagcacc agcaccatca gactggcaac
    12241 cgccgcacgg ttatgaataa aacgtcgacg tgcgtcctgc cacaagctgc gcccttcgac
    12301 ctccagcttt tcactgaaat tttccagcgt ctcgctgttt ttcttactta acatcatcgc
    12361 gagctccagt atcagtaacg gattttcggg tcgataaccg catatagcac atcgacaatg
    12421 gcattaaaca aaatggttaa agcaccaacc aggatggtca ggcttaacac taaggaatag
    12481 tcacggttca atgcgccatt aacgaacagt tgtccaatcc ccggcaaacc ataaatggtt
    12541 tcgataacca tagaaccggt aataatgccg acaaatgcgg ggcccatata agagagcacg
    12601 ggtaacagag caggttttaa tgcgtgacgt aaaatgatcc gccgcatagg taaccctttc
    12661 gcccgggcag tacgaataaa attggagtgt aatacttcaa tcatagagcc acgggtaata
    12721 cgcgcaatac tggcgatata agcgagtgac aacgccacca tcggcaatat catgaattta
    12781 agcgccccac cattccagcc accgcccggc agccaatgca aaatgatcgc aaatatcatg
    12841 actaataatg gtgcaaccac aaaactgggg ataacaaccc cggtcattgc cagccccatc
    12901 acggtatagt cccatttggt gttttgtttt aatgcggcaa taacgccagc actaacaccc
    12961 agtattaccg caaggaaaaa tgctgcggct cccagtttgg cagaaacggg aaaactggat
    13021 gcaaccaagt cattgaccga ataatcttta tatttaaacg atggaccgaa atcaccgtgc
    13081 gccagttgtt tcaggtagct gaaatactgt gtcatgatcg gatcattaag atgatatttc
    13141 gcttcgatat tggccatcac ttctggcggt aaagtacgtt cgccggtaaa agggcttccc
    13201 ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa taaatagcgt cggaatcgct
    13261 tccagacagc gacgtagaat aaattttaac attgccctac cttctggcct gtgccttctg
    13321 taatgcgata aaaatcagac accgtggagc aggacactcc tgccccacgt attgccatta
    13381 gtgcttcaca atgtacatat tccgggtata ggtattatcc agcggatctt tgccggtata
    13441 gccaccaacc cacggtttca ccagacgcgc attcacgtag taataaacag gaacaatggc
    13501 cgaatcctta tccagctgtt gttctgcttt agtgtacaga gctgtgcgct gcgcctcgtc
    13561 agtcactttc agcgtttccg ccataatgct gtcaaaggcc gggctcttat aatgcgcggt
    13621 attcatcgag ctgttcgaaa gcatggtgtt caggaaggaa gttggttcgt tgtagtcagc
    13681 acaccagcct gcacgggcca catcaaaagt accctggtga cgggtgtcga ggaacgtttt
    13741 ccactcctgg ttaaccagtt tgacgtttac accaatgttt ttcttccaca atgaagaggc
    13801 agcaatcgcc agctttttat gcagatcgga ggtgttatac aacaggttga ttgtcaacgg
    13861 tttgtctgcg gtataacccg cttcagccag cagttttttc gcttcttcgt tacgtttttc
    13921 ctggctccag ccaaaccatt ccggctgagt caattttgcg ccatcagtat acggtggagt
    13981 gtaaccatag gcgggcatgt cgccctgcgc tttcacttta ttaacaatga tatcgcggtc
    14041 catacctagt ttcagtgcgg tacgcacacg cacatcgttg aatggcggtt tctggttgtt
    14101 aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga acttcgtccg ggatctcttt
    14161 tttcagcttc tggaacaatt cgatcggcat gttgttataa gtcatgtcga tttcaccact
    14221 acggtagcgg ttgacatcgg taacttcaga agcaataggc aaataggtta cctggttaat
    14281 aacggttttc gcgttgttcc agtaggtcgg gctgcgttca agaacgattc gttcgtttac
    14341 gacccaatct tttaaggtat aggcaccgtt ggtgacgata ttaccaggct gggtccattt
    14401 ttcgccgaat ttctcgatag cggcttttgg caccggtgaa gttgatgggt gaacaagtaa
    14461 tttatagaag tacggaacgg gttcacttaa ggtgacttct aatgtgtgat catcaatagc
    14521 tttcacaccg agatcggtaa tcggtttttt cccttcaaga atttcatcaa taccggcgat
    14581 atgcccatat tgcagataac tggcatacgg agaagcagtg ttcggatcaa cagaacgttg
    14641 ccagctatac acaaagtctt gtgctgtgac tggcgtacca tcagaccatt tcgcatcttt
    14701 acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc caggattcag cgacgccagg
    14761 tgccggatga ccgtcaagat cgctgaccag taagccttca aacaggtctc ggctgatatt
    14821 agactccgga acaccttcaa ttttgtgcgg atctaatgac tgaacttctg aaccattgtt
    14881 acgtaccagc gtttgttttt ccgccagtgt gacgcctgcg ggtacatcag ctgccagcgc
    14941 gacattccct gccattagcg cagccagaac gccagctgct actaaacttc tcttggtgat
    15001 gttggtcatt gttttttgga ctccctcatt ataattactg gtattaacca gcatgtgtaa
    15061 tcccggattg ggaccctgca caggctcgga gactttctgc tgtcaggtta ctttatcgtt
    15121 acggattgct atcaccgact ttatttattc tggtgattca gacgatcacc ttatgtcgat
    15181 tcgtactgtg tctcccctgt cgagacacat tattctttcg cgacgctctt taattgttaa
    15241 ttgttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
    15301 tactaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagca attctctttt
    15361 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
    15421 tcaaatagag attggctaag catgtctatc ttttctgatt acagtttcag tattgaaatg
    15481 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg cagtggaatt
    15541 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
    15601 tgatggtgag gagaagcagt taagaaaatc cttataacta actgtaattt gagaagaata
    15661 tttttaccta ttccttttgc ttttgataac tgcgc
//
LOCUS       DAJTOR010000087        15666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTOR010000087 DAJTOR010000000
VERSION     DAJTOR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.087"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..948
                     /locus_tag="OO249_002449"
     CDS             <1..948
                     /locus_tag="OO249_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5636714.1"
                     /translation="ELTTNTMKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLH
                     GETLYLDNPRSNKKGRTVRTVDSYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIK
                     PDPLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNL
                     TILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKL
                     HFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANP
                     AFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
     gene            1000..1935
                     /gene="ttcA"
                     /locus_tag="OO249_002450"
     CDS             1000..1935
                     /gene="ttcA"
                     /locus_tag="OO249_002450"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5636715.1"
                     /translation="MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGIPHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2064..3437)
                     /gene="dbpA"
                     /locus_tag="OO249_002451"
     CDS             complement(2064..3437)
                     /gene="dbpA"
                     /locus_tag="OO249_002451"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5636716.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3883..4866)
                     /gene="zntB"
                     /locus_tag="OO249_002452"
     CDS             complement(3883..4866)
                     /gene="zntB"
                     /locus_tag="OO249_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5636717.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5121..6353
                     /gene="dgcM"
                     /locus_tag="OO249_002453"
     CDS             5121..6353
                     /gene="dgcM"
                     /locus_tag="OO249_002453"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR5636718.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWRGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6374..6937)
                     /gene="smrA"
                     /locus_tag="OO249_002454"
     CDS             complement(6374..6937)
                     /gene="smrA"
                     /locus_tag="OO249_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR5636719.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7267..8175)
                     /locus_tag="OO249_002455"
     CDS             complement(7267..8175)
                     /locus_tag="OO249_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5636720.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            8351..9651
                     /gene="abgA"
                     /locus_tag="OO249_002456"
                     /pseudo
     CDS             8351..9651
                     /gene="abgA"
                     /locus_tag="OO249_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
     gene            9651..11096
                     /gene="abgB"
                     /locus_tag="OO249_002457"
     CDS             9651..11096
                     /gene="abgB"
                     /locus_tag="OO249_002457"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR5636721.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANFPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQIQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTLSSLK"
     gene            11133..12659
                     /gene="abgT"
                     /locus_tag="OO249_002458"
                     /pseudo
     CDS             11133..12659
                     /gene="abgT"
                     /locus_tag="OO249_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            12670..13185
                     /gene="ogt"
                     /locus_tag="OO249_002459"
     CDS             12670..13185
                     /gene="ogt"
                     /locus_tag="OO249_002459"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5636722.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREIWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13380..14132
                     /gene="fnr"
                     /locus_tag="OO249_002460"
     CDS             13380..14132
                     /gene="fnr"
                     /locus_tag="OO249_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5636723.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            14284..15234
                     /gene="uspE"
                     /locus_tag="OO249_002461"
     CDS             14284..15234
                     /gene="uspE"
                     /locus_tag="OO249_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5636724.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFEPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(15284..15542)
                     /locus_tag="OO249_002462"
                     /pseudo
     CDS             complement(15284..15542)
                     /locus_tag="OO249_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
BASE COUNT         4036 a         3905 c         3839 g         3886 t
ORIGIN      
        1 gagttaacaa caaacacaat gaagaaaacg aaatcacaat taaaaacact caggataata
       61 atttgtgaga gtaccccgat atcgcatatt cgttatagcg atatcttaaa ctaccggaat
      121 gaactgctgc atggagaaac gctttaccta gataatccaa gatccaacaa aaaaggaaga
      181 accgtgcgca cagttgatag ctatatcgcc ctgctctgtt cgttgttacg ttttgcgtat
      241 cagtcgggat ttatatcaac caaaccattt gaaggagtaa aaaaattaca gcgaaacaga
      301 ataaaacctg acccgttatc taaaacagaa ttcaatgcat taatggaaag tgaaaaagga
      361 cagagccaaa acttgtggaa atttgccgtt tactccgggc ttcgtcacgg ggaactggca
      421 gctctggcgt gggaggatgt ggatttcgag aagggaattg tgaatgtcag aagaaacctg
      481 acgatacttg atatgttcgg tcccccaaaa acaaatgccg ggatccggac ggtaacatta
      541 ctgcagccgg ctcttgaagc actgaaggag caatacaaac tgaccgggca tcatcgcaaa
      601 agcgaaatca ctttttatca tcgggagtac ggcagaactg aaaagcaaaa actgcatttt
      661 gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt attactcggt aagcagtttg
      721 ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc gccgccgtaa tccgtaccat
      781 acgcgacata cttttgcctg ctggctgttg acggcaggag cgaacccggc atttatagcc
      841 agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa tttacggtat gtggattgat
      901 gacatgaacg acgaacagat agccatgttg aatgcgcggt tatcgtagtt gcaaagtttg
      961 cccccaattt gccccattta gtaccagaga actgaaataa tgcaagaaaa tcaacaaatt
     1021 acaaagaaag aacaatacag cctgaacaaa ttacaaaaac gtctacgtcg taacgtgggc
     1081 gaagccattg ctgacttcaa tatgattgaa gaaggcgatc gcatcatggt ttgcctctca
     1141 gggggtaaag acagctatac catgctggag attctgcgca atttgcagca aagcgcgcca
     1201 atcaattttt cgctggtggc tgttaacctc gatcaaaagc aaccgggctt cccagaacac
     1261 gttctgcccg tgtatcttga aaagctgggc gttgagtaca agattgtcga agagaacact
     1321 tacggtatcg tgaaagagaa gattccagag ggcaaaacca cttgctcact gtgttctcgc
     1381 cttcgtcgcg gtatccttta tcgtaccgca acggaactgg gggcgacgaa gatcgcgctg
     1441 ggtcaccatc gtgacgatat cctgcagacg ttgttcttaa atatgttcta cggcggcaag
     1501 atgaaaggta tgcctccgaa actgatgagc gatgatggca aacatatcgt tattcgtccg
     1561 ctggcctact gccgcgagaa agatattcag cgatttgccg atgcaaaagc gttcccgatt
     1621 attccgtgca acctgtgcgg ttcacagcct aacctgcaac gtcaggtgat tgctgacatg
     1681 ttgcgtgact gggataaacg ttatccaggg cgtatcgaga cgatgttcag cgcgatgcag
     1741 aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg atttcaaagg catcccccac
     1801 ggttctgaag tggttaacgg gggtgatctg gcgtttgatc gcgaagagat cccactacaa
     1861 ccggcgggct ggcagccaga agaagatgaa aatcagttgg atgagttacg gctgaatgtg
     1921 gttgaagtga aataaccagg atagcgcccg atgcgcaagc gtatcgggct actcttatgg
     1981 aggccggata agacgcggcc aacgtcgcat ccggcaatgc cgaagaagat gtttactctt
     2041 gcacccggca attcaacatt tcattatttt aataaccgca cccggcacgt ttttccttta
     2101 atcttcccgc cctgtaactg tttccatgct ttatgagcaa cagcctgacg gaccgcgaca
     2161 tagacatgcg ccggatgcac ggcgattttg ccaatatctg cgccatcaag cccgatatct
     2221 cctgtcagtg cacctaatac atcacccggg cgcattttgg cttttttccc gccatcgata
     2281 cacaacgttg ccatttctgc ttccagcggc acaatggaac tattagctgg cggcgtttgc
     2341 cagttaagtt ttatctgcaa catgtcagaa atgatattgg cccgctgtgc ttcttccgga
     2401 gcacagaaac tgatcgccag accgctattt cctgcacgag ctgtacggcc gatgcgatgt
     2461 acatgaactt cagggtccca cgccagctca aagttcacca ccagctcaag cgatttaata
     2521 tccagaccac gcgcagcaac atcagtcgca accagtacac gggcgctacc gttagcaaaa
     2581 cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc cgtgtaatga caatgcactt
     2641 tgccctactt cattcagcgc gtcgcagaca gcctggcaat cttttttggt attgcaaaac
     2701 accacgcaag aggatggctg atgcaagctt aataaccgtt gcaacagagg aattttgcct
     2761 ttgctggatg tctcataaaa ttgttgttca atgggtggca aagcatctgt tgagtcaatt
     2821 tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta cggcgatggc ttccggccag
     2881 gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa aacggatgac atcatcaatg
     2941 gcatcgctaa atcccatatc cagcatgcgg tcggcctcat ccatcaccaa agtattcagc
     3001 gcatccagtg ataccgtgcc tttttgcaag tgatccagca aacgacccgg cgttgccacg
     3061 ataatatgcg gcgcatgttg caacgaatca cgctgcatac cgaacggttg accaccgcac
     3121 aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc gacgcaattc acctgccacc
     3181 tgatccgcca gttcacgcgt aggacacagc actaaagcct gggtttgaaa tagcgacgca
     3241 tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt tgccgctgcc ggttttcgcc
     3301 tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg cggcggcctg caccggcgtc
     3361 atagttaaat aacccaactc attaaggttc gtgagttggg cgggaggcaa aacattcagg
     3421 gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac gcaaagttag caggcgcgta
     3481 tcctcgcaga tctacgcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg
     3541 ggcactggaa caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt
     3601 gtcgtcatat ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa
     3661 caggcaaaaa aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag
     3721 taattcaaaa aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt
     3781 cacctgcaag ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt
     3841 tgcggcgttt ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg
     3901 atgcaaccat aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa
     3961 tccgaattgc cacccgccgc cagggatccc accaaggttg acgccaaata accctgtcag
     4021 aaaggtactg ggtaaaaaga ccattgccat caatgacatt gtataggtac gacgagctaa
     4081 attttcctgc atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc
     4141 gtcgatttcg tcaagaccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg
     4201 gtcatcgctc atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg
     4261 tgccatatag cgacgcatca cgattaattg tttgcgcagc agagccagga atccacgcgg
     4321 tggaatttgc tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc
     4381 gataaattcg ctggaatgat cggtcaacgc atcgcacaca tccaccagcc accctccgca
     4441 atcggtcgga cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt
     4501 gcgttgtcgg gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg
     4561 atcggggcgt tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc
     4621 gagacggctg actcggggcc gtgtgctctc gcctgccagc gcatcacgta cgttattggg
     4681 aagcagcggt gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag
     4741 ccagcaggga tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct
     4801 accatccagc atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc
     4861 ttccacagtg cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac
     4921 gcaacatctc atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata
     4981 caaaaagcaa aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat
     5041 tatcagaatt atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg
     5101 ataaaaataa cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca
     5161 gtcctgtctg gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc
     5221 gactgttgat gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct
     5281 cacaaaaaga actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa
     5341 tcctcactgt tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag
     5401 agctgacaga aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg
     5461 gtttaaaagc cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct
     5521 tttttctgac taactctgca ccaatgttgt tgattgaccc ctcacgagat ggccaaatcg
     5581 tcgatgctaa cctcgccgcg ctcaatttct acggttataa ccatgaaacg atgtgccaga
     5641 aacatacctg ggaaattaat atgctcgggc gtcgcgtcat gcctatcatg catgaaattt
     5701 cgcatttacc cggcggtcat aaacctttga attttgttca taaactggcg gatggatcta
     5761 ctcgtcatgt gcagacctat gccgggccga ttgaaattta tggcgacaag ctcatgttat
     5821 gtattgtgca tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc
     5881 accatgacgc gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag
     5941 gccaaatgca gcacctcgcc atcgcccagg attacagctt gttgctcatc gacaccgatc
     6001 gttttaaaca cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc
     6061 tcgcccgcac cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggagaggcg
     6121 aagagtttgt cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa
     6181 ctatccgtgt aagcgtggcc aaagtgagta tttcgggctt accacgtttt accgtcagca
     6241 ttggtgtggc gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg
     6301 atgctttgta tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg
     6361 atgcgtctcg agatcaacgg ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct
     6421 gcgccgtttt acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg
     6481 tgcaatatgc ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa
     6541 tattggcatg cgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac
     6601 catccgccag ggcttggtga atgaaactga acaccatttt gcggcactct tccaccggct
     6661 ggcgcaaaag attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca
     6721 ccccatgttg caacccttcc cgccgaaact ccagcggctg acttagcggg atgatgtcga
     6781 gaaatccggt ggtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt
     6841 ggttacgcgt tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca
     6901 tggcgtcaag aaacagcgat ttgtcgtcaa ggttcatgtt acatcctccc gcaattaaga
     6961 gcgcgatatg ataaccagac cgggtcggtc caacaacgta ttaccaaaaa ttccagtaat
     7021 aagttccaaa tattgccgat attttaagca aaatgcttat gcatgattat tcattcacga
     7081 tattaataat gtaacttata ttttcgtgaa atctgtcact gaagaaaatt ggcaactaaa
     7141 ggttaaaacc gttataacac agtcaccggc gcagaggaga caatgccgga cttaagacgc
     7201 ggatgcactg ctgtgtgtac tgtagagtct ggcggatgtc gacagactct atttttttat
     7261 gcagttttaa ctttgcagat agccgcattc tcgccggaat tgcgtgatta atgacggggt
     7321 cagtggtgtc tggcgactat cacgccgctg aatcaaataa taggccgctt tcggtaaaat
     7381 ttcgctaacc ggcaacatca ccagcccctg tccgtgcaag ggatcgcagc ccatttcttc
     7441 aggcagtatg ctgagaaaat cgctttttgc caccagactg atacaggctg agaacgtctc
     7501 gcagactaca ccgacctgtg gcgtttgcgc ctgatcgtca agcaattcac tcaactgttt
     7561 gtagtaactg ccgtgtggcg tcggcattgt ccagctgtaa tccagtaact gtttgatcga
     7621 acgggcacca atggcggggt gtcccgggcg gcaaaagatc gcgaattgct tttccagtaa
     7681 tttctcaaaa gtaaattcgt ggtcgtacgg tccctgataa taggtattga tggtgaaatc
     7741 caattctccc tgacgcaatt cattaatcat cgacaccagt tgcccttcca taatgcgtac
     7801 ttttacctgc ggatgctgct gatgaaaacg agatatgaca gctggcatca gactgcggga
     7861 aatactggcc cccatgccga tattaatctg ccctgccagt tgcccttgtc gttggcgaat
     7921 atcctcttgg gctgcgcgca gctcttcaag aataagactg gcgtgctgat aaaaactttc
     7981 accggcatca gttaacgtca cgcctttact acggcgaaaa aagagttgcg ccgctaatcc
     8041 ttcttccaac tcctgaatag atttactcag tgccggttgc gacatattca acattcggct
     8101 cgctccgcga atgctgccct gacgagccac ttcaacaaaa gcacgaattt gatgaatttt
     8161 tacctgaaaa gccatgacgc caccgataac cgttatttat cagaccaaag aaactggcat
     8221 ctactttaat gcagatgatt gtgtcagggt aatttatgaa cggttaaaac tgtgaaaaat
     8281 cagttagtga taagtaaaaa ctatcgctac gtgaaccggg tcacactttt tactgatgac
     8341 gggaaaggtt atggagtctt tgaatcaatt tgttaattcg cttgccccaa aattatcgca
     8401 ctggcgacgt gattttcatc actatgcaga gtctggctgg gtggaattcc gcactgccac
     8461 ccttgttgcg gaagaattgc agcagctcgg ctattcactg gcgctgggcc gcgaagtagt
     8521 taatgaaagt agccggatgg gactacctga tgaactcact ctacaacgcg aattcgagcg
     8581 cgctcgtcaa cagggtgcgc tagcacaatg gattgcggtt tttgaaggtg gtttcactgg
     8641 tatcgtcgcc accctggata ccggtcgccc cggtccggtg atggctttcc gtgtcgatat
     8701 ggacgcgctg gatctcagtg aagagcagga tgtcagccat cgcccctacc gcgacggttt
     8761 tgcgtcatgt aacgccggaa tgatgcatgc ctgtggtcat gatggacata ccgccattgg
     8821 gcttgggcta gcgcataccc ttaaacaatt cgagtccgga ctacatggcg tcatcaaact
     8881 gatttttcag cctgcagagg aaggtacgcg tggcgcgcgg gcgatggtcg atgcaggtgt
     8941 cgtagatgat gttgattatt ttactgccgt gcacattggc actggcgtac ctgcgggcac
     9001 cgtggtgtgc ggcagtgata attttatggc aaccaccaaa tttgacgcgc acttcaccgg
     9061 taccgccgct cacgcaggcg caaaaccaga agacggtcac aatgccttgt tggcggcagc
     9121 acaagccact cttgcactgc atgcaatcgc cccggagctt ccagagtaaa cgtgggcgtt
     9181 atgcaggcag gaagcggtcg taacgttgtt cctgcctcgg cgttgctgaa agtggaaaca
     9241 cgcggggcca gcgacgtcat taatcaatat gtttttgacc gtgcacaaca agcgattcag
     9301 ggcgcagcaa ccatgtatgg tgtcggcgtt gaaactcgtc tgatgggtgc agctaccgcc
     9361 agttctcctt cgccgcaatg ggtcgcatgg ttgcaaagtc aggcggctca ggtcgcgggg
     9421 ggcaatcagg ccattgaacg tgttgaagcg cctgcgggtt ccgaagatgc cacattaatg
     9481 atggcccgcg tgcagcaaca tcaagggcaa gcctcctacg tggtgtttgg cacacagctg
     9541 gcggcaggtc atcacaacga aaaattcgat tttgacgagc aggttctcgc tattgccgtc
     9601 gaaacgctgg cgcgcaccgc gctcaatttt ccctggacgc gaggtatctg atgcaggaaa
     9661 tctatcgttt tatcgacgat gcgattgaag ccgatcgcca acgttatacc gatattgccg
     9721 atcaaatctg ggatcatcca gaaacacgtt ttgaagagtt ctggtcagcg gagcatctgg
     9781 cttcggcgct ggaatctgca ggcttcaccg ttacccgcaa cgtaggctat atcccaaatg
     9841 cctttattgc ttcgtttggt caaggcaaac cggttatcgc cctgctggga gaatatgacg
     9901 ccctggcagg tttaagtcag caagcaggtt gcgcgcaacc tacatccgtg acgcccggtg
     9961 aaaatggtca cggttgcgga cacaatttgc tgggaaccgc cgcctttgcc gctgcaatag
    10021 ccgtcaagaa atggctggaa caatatgggc aaggcggcac ggtgcgcttt tatggttgtc
    10081 ctggcgaaga aggcggctcg ggtaaaacgt tcatggttcg cgagggggta tttgatgatg
    10141 tggatgcggc actcacttgg cacccggaag cctttgccgg tatgttcaat acccgcacgc
    10201 tggcaaacat tcaggcatca tggcgcttta aagggatcgc agcacatgcc gcgaatttcc
    10261 ctcatttggg acgcagcgcc cttgatgccg taacgttgat gaccactggc accaacttcc
    10321 tcaacgaaca tattattgaa aaagcgcgcg tacactatgc catcacaaat agcggcggga
    10381 tctcgcccaa cgtggtccag gcgcaggcag aagtgcttta tcttatccgc gcccccgaaa
    10441 tgaccgacgt gcagcatatt tatgatcggg tcgccaaaat cgccgaaggt gcggcattga
    10501 tgaccgaaac cacggttgaa tgccgcttcg acaaagcctg ttccagttat ctcccgaatc
    10561 gcaccttaga aaatgccatg taccatgccc tatcccattt tggtaccccg gaatggaact
    10621 ccgaagaact ggcttttacg aaacaaattc aggctacgct cccccccaac gatcggcaaa
    10681 acagtctgaa taatatcgcc gcaaccggtg gcgaaaacgg caaggctttt gcactacgtc
    10741 atcgtgaaac ggtactggcg aatgaggtcg ctccatatgc cgccaccgat aacgtgcttg
    10801 cggcatcgac tgatgtcggc gacgtcagtt ggaaactgcc tgttgcccag tgtttcagcc
    10861 cctgttttgc cgtcggtaca ccgctacata cgtggcaact ggttagccag gggcgaacat
    10921 ctattgctca taaaggaatg ctgctggcgg cgaaaactat ggcagcaacc acactcaatc
    10981 tcttcattga ttcagggcta ttgcaagaat gccaacaaga gcatcagcaa gttacggaca
    11041 cgcaaccgta tcactgccct atcccaaaaa acgtgacact gtcatcttta aaataacaac
    11101 aacaacgcaa acacaacaac cgaggaatgc ccatgagtat gtcatccata ccgtcgtcct
    11161 cctaatccgg gaagctctat ggctgggtcg aaagaattgg taacaaggtt ccccatcctt
    11221 ttttgctctt tatctatttg attatcgtac tcatggtgac gacggcaatt ttgtcggcct
    11281 ttggcgtcag tgcgaaaaac ccgaccgatg gaacgccggt ggtggtgaaa aacctgctca
    11341 gtgtggaagg attacactgg tttttaccca atgttattaa aaactttagc ggttttgctc
    11401 cacttggtgc gatcctggcg ctggttttag gtgccggtct ggcggagcgc gtcggcttac
    11461 tgccagcgct aatggttaaa atggcatcgc atgttaatgt ccgctacgcc agttatatgg
    11521 tgctgtttat tgcttttttc agccacattt cttccgatgc ggcgttagtg atcatgccac
    11581 cgatgggtgc gctgattttt ctggcggtgg gcaggcatcc agttgcaggt ttactggctg
    11641 ccattgcagg cgtaggttac ggctttacgg ctaatttact gattgtcaca accgacgtgt
    11701 tgctgtcggg gatccgcacg gaagcggcgg ctgcgttcaa tccgcaaatg cacgtcagtg
    11761 taattgataa ctggtatttt atggccagct ccgtagtcgt actgacgatt gttggcggcc
    11821 tgataaccga caaaatcatc gagccacggt taggtcaatg gcagggaaac agcgatgaga
    11881 aactgcagac attgaccgaa agtcagcgtt ttggtttacg catagcaggt gtcgtatcgc
    11941 tactttttat tgctgcgatc gcgctgatgg tgatcccgga aaacgggata ttgcgcgatc
    12001 cgattaatca caccgtgatg ccatcaccct ttattaaagg tatcgtgcca ctaatcattc
    12061 tttttttctt tgtggtttcg ctggcttatg gcatcgctac ccgcacaatt cgacatcagg
    12121 cggatttacc gcatttaatg attgaaccga tgaaagagat ggcgggattt atcgtgatgg
    12181 tttttcccct cgcccagttt gtcgccatgt ttaactggag caacatgggg aaattcatcg
    12241 ccgtggggct gaccgatata ctggaaagtt cagggcttag cggcatcccg gcgtttgtcg
    12301 gtctggcgtt gctttcctct ttcttatgca tgtttattgc cagtggttcc gcaatctggt
    12361 cgattctggc ccccattttc gtaccaatgt ttatgctact tggctttcac ccggcatttg
    12421 cgcaaatcct ctttcgtatt gccgactcat ccgtattgcc tttagcgccg gtatctcctt
    12481 ttgttccact gtttcttgga ttcctgcaac gctacaaacc agacgcgaaa ctgggtactt
    12541 actattcgtt agtcttgccc tatccgctta tctttttggt ggtatggctg ctgatgttgc
    12601 tggcgtggta tcttgttggt ctgccgatag gtccggggat ttacccacgt ttgtcttaag
    12661 agagaacgga tgctgagatt acttgaagaa aaaattgcca cgccactggg tccactgtgg
    12721 gtgatttgcg atgagcaatt tcgcctgcgg gcggttgaat gggaagagta cagcgaacgc
    12781 atggtgcagc tgctggacat ccattatcgc aaagaaggct atgagcgcat ttctgccacc
    12841 aacccaggcg gtttaagcga caagcttagt gattattttg ccggtaatct tagcattatt
    12901 gatacgcttc ccactgccac aggggggacg ccatttcagc gcgaaatctg gaaaactcta
    12961 cgcactatcc cctgcgggca ggtaatgcat tacggccaac tggctgaaca attgggccgc
    13021 cctggcgcgg cgcgtgccgt tggtgcggca aacggatcga atcccatcag catcgtcgta
    13081 ccttgccatc gggttattgg ccgaaacggc accatgaccg gatatgcagg cggagttcag
    13141 cgaaaagagt ggttattgcg ccacgaaggt tatcttttgc tgtaaacatt aaacaatttg
    13201 tgccagcttg ttcacacttt tatgtaaagt tacccttaac aacttaatgg ttttcaaata
    13261 gatagacata tatttacatc taatatcgga attctctgct gttaaggttt gcttagactt
    13321 acttgctccc taaaaagatg ttaaaattga caaatatcaa ttacggcttg agcagaccta
    13381 tgatcccgga aaagcgaatt atacggcgca ttcagtctgg cggttgtgct atccattgcc
    13441 aggattgcag catcagccag ctttgcatcc cgttcacact caacgaacat gagcttgatc
    13501 agcttgataa tatcattgag cggaagaagc ctattcagaa aggccagacg ctgtttaagg
    13561 ctggtgatga acttaaatcg ctttatgcca tccgctccgg tacgattaaa agttatacca
    13621 tcactgagca aggcgacgag caaatcactg gtttccattt agctggcgac ctggtgggat
    13681 ttgacgccat cggcagcggc catcacccga gcttcgctca ggcgctggaa acctcgatgg
    13741 tatgtgaaat cccgttcgaa acgctggacg atttgtccgg taaaatgccg aatctgcgtc
    13801 agcagatgat gcgtctgatg agcggtgaaa tcaaaggcga tcaggacatg atcctgctgt
    13861 tgtcgaagaa aaatgccgag gaacgtctgg ctgcattcat ctacaacctg tcccgtcgtt
    13921 ttgcccaacg cggcttctcc cctcgtgaat tccgcctgac gatgactcgt ggcgatatcg
    13981 gtaactatct gggcctgacg gtagaaacca tcagccgtct gctaggtcgc ttccagaaaa
    14041 gcggtatgct ggcagtcaaa ggtaaataca tcaccatcga aaataacgat gcgctggccc
    14101 agcttgctgg tcatacgcgt aatgttgcct gatttttccg cataactcac tatccttctg
    14161 tcatatcatt aaatttttct gatttattga tctggcagaa ggttcatcac tgtttcattc
    14221 accagatatg ggttaatctt ttaattacaa actgcgttga cagttgttgt aaggagaccc
    14281 tgtatggcta tgtatcagaa catgctcgtt gttatcgatc ctaaccagga cgaccaacca
    14341 gcattgcggc gagctgttta tttacatcaa cggattggtg gcaaaattaa agcctttttg
    14401 ccgatctatg acttctcata cgaaatgacc accctgctct ccccggacga acgtaccgct
    14461 atgcgtcagg gcgtcatcag ccagcgtaca gcctggatcc acgagcaggc aaaatattat
    14521 ctcaatgctg gcgttcccat tgaaattaaa gtggtctggc ataaccgtcc tttcgaagcc
    14581 atcattcagg aagtgatcag cggcgggcac gatttggtgc taaaaatggc gcaccaacat
    14641 gaccgtctgg aagcggtgat ttttacgcca acggactggc atctgttacg caaatgccca
    14701 agcccggtgt ggatggtgaa agaccagccg tggccggaag gaggtaaggc gctggtggcg
    14761 gtgaatctcg ccagtgaaga gccgtatcat aatgcgctca atgaaaaact ggtcaaagag
    14821 acgatcgaac tggcagaaca agtcaaccat accgaagttc atctggttgg cgcttaccca
    14881 gtaacgccaa ttaatatcgc gattgaactg ccggaatttg agccgagcgt ttataacgat
    14941 gccattcgtg ggcaacattt gctggcaatg aaagccctgc ggcagaaatt cggcattaat
    15001 gaaaacatga cgcacgtaga aaaaggtctg cccgaagagg ttattcctga tttggcggag
    15061 cacttacagg cggggattgt ggttctgggc acggtaggac gcaccggtat ttcagcagca
    15121 ttcctcggca acacggcgga acaggtgatt gatcatcttc gctgcgatct gttggtcatt
    15181 aaacctgacc agtatcagac acccgttgaa ctggatgacg aagaagacga ttaacgactc
    15241 ttcaataact gacaaggccc gagttatcgg gcctattttt tacttactgc tcttcgccgc
    15301 caaggaaata aattcccaac gggattgcca gcaacacagt aaagaccagg ctataaacaa
    15361 aaatcatcga tgactgaatc acatacattg atgtcgtcca ctcagacagc ggaatattgt
    15421 actgttctat aacgccgccg atagtcgcgc gagtcacaac cgacgccgca ataatgacaa
    15481 accgccaaca aaccaaagag aaatttcttc cctttcgctg acttcagttt cgccataatc
    15541 atgatgtaat ccttgtgcag acaaatttct tgttacctaa acgtaacaat accatgacat
    15601 ttcaccgttg tctgctctga aaacctcacc tcttagctgg atttacaatc aacgcattga
    15661 ttagtt
//
LOCUS       DAJTOR010000088        15659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTOR010000088 DAJTOR010000000
VERSION     DAJTOR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.088"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            86..1582
                     /gene="ravA"
                     /locus_tag="OO249_002463"
     CDS             86..1582
                     /gene="ravA"
                     /locus_tag="OO249_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5636725.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARALEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            1576..3027
                     /gene="viaA"
                     /locus_tag="OO249_002464"
     CDS             1576..3027
                     /gene="viaA"
                     /locus_tag="OO249_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5636726.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3032..4024)
                     /gene="asnA"
                     /locus_tag="OO249_002465"
     CDS             complement(3032..4024)
                     /gene="asnA"
                     /locus_tag="OO249_002465"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5636727.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYLDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4176..4634
                     /gene="asnC"
                     /locus_tag="OO249_002466"
     CDS             4176..4634
                     /gene="asnC"
                     /locus_tag="OO249_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5636728.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQHPIMRTIKP"
     gene            4724..5167
                     /gene="mioC"
                     /locus_tag="OO249_002467"
     CDS             4724..5167
                     /gene="mioC"
                     /locus_tag="OO249_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5636729.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5546..7435
                     /gene="mnmG"
                     /locus_tag="OO249_002468"
     CDS             5546..7435
                     /gene="mnmG"
                     /locus_tag="OO249_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5636730.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7499..8122
                     /gene="rsmG"
                     /locus_tag="OO249_002469"
     CDS             7499..8122
                     /gene="rsmG"
                     /locus_tag="OO249_002469"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5636731.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8739..9119
                     /gene="atpI"
                     /locus_tag="OO249_002470"
     CDS             8739..9119
                     /gene="atpI"
                     /locus_tag="OO249_002470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5636732.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9128..9943
                     /gene="atpB"
                     /locus_tag="OO249_002471"
     CDS             9128..9943
                     /gene="atpB"
                     /locus_tag="OO249_002471"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5636733.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9990..10229
                     /gene="atpE"
                     /locus_tag="OO249_002472"
     CDS             9990..10229
                     /gene="atpE"
                     /locus_tag="OO249_002472"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5636734.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10291..10761
                     /gene="atpF"
                     /locus_tag="OO249_002473"
     CDS             10291..10761
                     /gene="atpF"
                     /locus_tag="OO249_002473"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5636735.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10776..11309
                     /gene="atpH"
                     /locus_tag="OO249_002474"
     CDS             10776..11309
                     /gene="atpH"
                     /locus_tag="OO249_002474"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5636736.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11322..12863
                     /gene="atpA"
                     /locus_tag="OO249_002475"
     CDS             11322..12863
                     /gene="atpA"
                     /locus_tag="OO249_002475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5636737.1"
                     /translation="MQLNSTEISELIKLRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12914..13777
                     /gene="atpG"
                     /locus_tag="OO249_002476"
     CDS             12914..13777
                     /gene="atpG"
                     /locus_tag="OO249_002476"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5636738.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHLYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13804..15186
                     /gene="atpD"
                     /locus_tag="OO249_002477"
     CDS             13804..15186
                     /gene="atpD"
                     /locus_tag="OO249_002477"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5636739.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15207..15626
                     /gene="atpC"
                     /locus_tag="OO249_002478"
     CDS             15207..15626
                     /gene="atpC"
                     /locus_tag="OO249_002478"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5636740.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
BASE COUNT         3859 a         3937 c         4108 g         3755 t
ORIGIN      
        1 cctttgagac tattttagcc accagcaaga aacgtctata ctcgcaattt acgcagaact
       61 tttgacgaaa ggacgccact tcattatggc tcaccctcat ttattagcgg aaagaatttc
      121 ccgcctgagc agttcgttgg aaaaggggct ttatgaacgt agccacgcca tccgcttgtg
      181 tttattagcg gcattaagtg gtgaaagtgt gttcctcctt ggcccgccag gtattgccaa
      241 aagtttgatc gcccggcgct taaaattcgc ctttcagaat gcccgcgcgc ttgaatatct
      301 gatgacccgc ttctccacgc cggaagaagt ttttggtccc ctttctattc aggcgctaaa
      361 agatgaaggg cgctatgaac gtttaaccag cggttacctg ccggaagcag aaattgtctt
      421 tctggatgag atctggaaag cgggcccggc aattcttaat accttgctca ccgccattaa
      481 cgagcgccag ttccgcaacg gtgcacacgt agaaaaaatc ccgatgcgtc tgctggtggc
      541 ggcctccaac gagctgccgg aagcagacag cagtctggaa gcgttatatg accgcatgct
      601 gattcgtctg tggttagata aagtgcagga taaagcgaat ttccgctcca tgctgaccag
      661 tcaacaggat gaaaacgaca atccggttcc tgatgccttg caggtcacag atgaagaata
      721 tgaacgctgg cagaaagaga ttggtgaaat tacgctgccc gatcatgtat ttgagctgat
      781 ttttatgctg cgccagcaac tggataaatt accggatgcg ccttatgtct cggatcgtcg
      841 ctggaaaaaa gcgatccgat tattgcaggc cagcgccttt tttagcggtc gcagtgctgt
      901 tgccccggta gatctcattt tgctgaaaga ttgcctgtgg tatgacgcgc aaagcctgaa
      961 tttgatacaa caacaaattg atgtattgat gaccggtcac gcctggcaac agcaagggat
     1021 gttgacccgc ctaggcgcga ttgtgcaacg tcacctgcaa cttcagcagc aacaaagcga
     1081 taaaacagcc ttaacggtaa ttcgtctggg cggcattttt agccgtcgtc agcagtatca
     1141 actccctgtt aacgttactg cttccactct gactctgctg ctgcaaaaac cgttaaaact
     1201 gcatgatatg gaagtggttc atatctcctt tgagcgtagc gcgctggaac agtggctgag
     1261 taaaggtggt gaaattcgcg gcaaactgaa cggtatcggc tttgcccaga aactgaatct
     1321 ggaagttgat agcacccaac atcttgttgt acgcgatgta agtttacaag gcagtacgct
     1381 ggcacttccc ggttcatcgg ctgaaggtct gccaggtgaa ataaaacaac aactggaaga
     1441 gcttgaaagc gactggcgta agcaacacgc tttattcagc gaacagcaaa aatgtctgtt
     1501 tatccctggc gactggttag gtcgcattga agccagccta caggatgtcg gtgcacagat
     1561 tcgccaggca caacaatgct aacgctggat acgcttaatg tgatgctggc cgtcagcgaa
     1621 gagggattga tcgaagagat gatcatcgca ctgctggcct caccgcagct ggcggtcttc
     1681 tttgaaaaat tcccacgact gaaggcagca atcactgatg atgttccccg ctggcgtgag
     1741 gcgctgcgca gtcggctgaa agatgcccga gtcccgccag aactcaccga agaggtgatg
     1801 tgctatcagc aaagccagct cctctccacg ccgcagttta ttgtgcagct accacagatc
     1861 ctggacttac tgcatcgtct gaattcccca tgggcagaac aagcccggca gttggttgat
     1921 gctaacagca caataacttc agcgttacac acgctttttc tccagcgctg gcgtttaagt
     1981 ctgatcgtgc aagcaacgac gttaaatcaa cagctattag aagaagaacg cgaacaactg
     2041 ttaagtgaag ttcaggaacg catgacgctg agcggacaac ttgaaccgat tctcgcagat
     2101 aacaataccg cagctggtcg tctgtgggat atgagcgccg gccagcttaa acgtggcgac
     2161 tatcagttga ttgtgaaata cggtgaattt cttaacgaac agccggaact gaaacgcctg
     2221 gcagagcagc tggggcgttc tcgggaagcc aaatcaatac cgcgcaacga tgcgcagatg
     2281 gaaaccttcc gcaccatggt gcgcgaaccg gcgacggttc ctgagcaggt tgatggtctg
     2341 caacaaagcg atgatatttt acgtctcctg ccgccagaac tggcgacact agggataacg
     2401 gaactggagt atgagtttta ccgtcggctg gtggaaaaac agttgctcac ctatcgcctg
     2461 cacggtgagt cgtggcgtga aaaagtgatc gaacgtccgg tggtacataa agattacgat
     2521 gaacagccgc gcgggccgtt tattgtctgt gtggatactt ccggctcaat gggcggcttt
     2581 aatgaacagt gtgcgaaagc gttctgcctg gccttgatgc gcattgctct cgcagaaaac
     2641 cggcgctgct atattatgct attttccacc gagatcgtcc gttatgagct ttcaggccca
     2701 caaggcatcg aacaagcaat ccgtttttta agccagcagt ttcgtggcgg caccgatctt
     2761 gccagttgtt ttcgcgccat tatggaacgc ttgcaaagca gggaatggtt tgatgccgat
     2821 gcggtggtga tttctgattt tatcgctcag cggttgcctg acgacgtgac gagtaaagtg
     2881 aaagagctgc agcgggtaca tcagcatcgc tttcatgccg tggcgatgtc ggcacacggc
     2941 aaacccggca tcatgcgcat tttcgatcat atctggcgct ttgataccgg gatgcgaagc
     3001 cgcctgctca gacgctggcg gcgataaatt attacagcag agaagggacg ctctcgcgaa
     3061 cagcagctgg ccatactcca cactgaacct ggccgatatg cggcagttgc agcagcagca
     3121 tagtcaaacg agactggccg ataccgccgc cgatggtctg cggcatttca ccgcgcagca
     3181 gcgcctgatg ccattccagc tgcagacgat cttcatcacc ggtcagcgcc agctgatgct
     3241 tcagcgtgtc ggcatccacg cggatcccca tggaagaaag ctcaaacgca tcttccagta
     3301 cagggttcca caccagaata tcgccgttca gacccgcatg acccagctct gacggggtgc
     3361 tccagtcatc ataatccggt gcgcgcacgt cgtggcgatg accatcgctc agcttgccgc
     3421 caatcccgac aaggaatacc gcgccaagat ctttcgctat cgcccgctca cgccctttgg
     3481 catcgagatc cagataacga gacagtaact cctggctgtg tacgaagtgg atctgatccg
     3541 gcaggaacgg tgccaggcca aactcttcgc taaccgcagc ttcggttgct ttaattcccg
     3601 cccagatcgc ctctaccgtg cttttcagag tcgagaattg acgctcaccg tcgcccatta
     3661 cgcgttccca gtcccactgg tctacataga ccgagtgcaa cggagaaaga cggtcttcat
     3721 cggggcgaag ggctttcatg tgcgtgtaca gcccttcgcc cgcgctgaag tcgtgttgcc
     3781 ctaaggtctg acgtttccac ttcgccagtg aatgaaccac ttcgaactgg gcatcaggca
     3841 gagctttcac ttttacctgc accgcttttt cacagcccga caagttatcc tgcgtgccat
     3901 cccccacacg gctaagaatc ggtgcctgga cttcgatcag ccccagacgt tcttccagtt
     3961 gacgagaaaa gtgagatttc acgaagctaa tttggcgttg tttggcaatg taagcggttt
     4021 tcatttttta tactcctgcg tcctgttgct tatgattaag caacaaaaac cgacacacat
     4081 gcaataatca ttcaataaaa agcctaacac acttttgatt cattaaaaaa gaaggctaaa
     4141 atagaatgaa tcatcaatct gcataagaaa atcctatgga aaattatctg atcgacaatc
     4201 tggaccgtgg catcctggaa gcattaatgg gcaatgcgcg caccgcttac gccgaactgg
     4261 cgaaacaatt tggcgtcagt ccggggacga ttcacgttcg ggtagagaaa atgaagcagg
     4321 cggggatcat taccggggcg cgtattgatg tcagcccgaa gcagctcggt tatgacgtag
     4381 gctgctttat cggcattata ttaaagagcg ccaaagacta cccttccgcg ctggcaaagc
     4441 tggaaagcct tgatgaagtc actgaagcct attacacaac aggccactac agcatcttta
     4501 taaaagtaat gtgccgttcg atcgacgctc tccagcatgt acttatcaac aagatccaaa
     4561 caattgatga aattcagtcc accgagacat tgatcgtcct gcaacacccg atcatgcgta
     4621 ctatcaagcc ctgatcggct tttttaatcc catacttttc cacaggtaga tcccaacgcg
     4681 ttcacagcgt acaatacgcc actcttaata aaggtggcgg tttatggcag atatcactct
     4741 tatcagcggc agcaccctcg gcggtgccga atatgtagca gaacacctgg ctgaaaagct
     4801 ggaagaggcg ggttttacca ccgaaacgct gcacggtccg ctgttggaag atttatctgc
     4861 ctcggggatc tggctgatta tcagctccac ccacggtgcc ggagatattc cggacaacct
     4921 ttctcctttc tatgaagcat tgcaggaaca gaagcccgat ctttctgcgg tccgctttgg
     4981 cgcaatcggt attggtagtc gtgaatatga caccttttgt ggggctatcg ataaactcga
     5041 ggcagaactc aaaaattccg gtgcaaaaca gacaggcgaa acactgaaga tcaacattct
     5101 tgatcacgac attccggaag atccggcaga agaatggctg ggatcgtgga ttaatttact
     5161 caaataagta tacagatcgt gcgttctact gtggataact ctgtcaggaa gcttggttca
     5221 accggtagtt atccaaggaa caacggttgt tcagtttttg agttgtgtat aacccctcat
     5281 tctgatccca gcttatacgg tccaggatca ccgatcattc acagttaatg atcctttcca
     5341 ggttgttgat cttaaaagcc ggatccttgt tatccacagg gcagtgcgat cctaataaga
     5401 gatcacaata gaacagatct ctaaataaat agatctcctt tttaataccc aggatcccag
     5461 gtctttctca agccgacaaa gttgagtaga atccacggcc cgggcttcaa tccattttca
     5521 taccgcgtta tgcgaggcaa tcaccatgtt ttatccggat ccttttgacg tcatcatcat
     5581 tggcgggggt catgcaggca ccgaggccgc gatggccgcg gcgcgtatgg gtcaacagac
     5641 tctgcttttg acacacaata tcgacactct ggggcagatg agctgcaacc cggcgatcgg
     5701 cggtattggg aagggacatc tggtaaaaga agtggatgca ctcggcggtc tgatggcgaa
     5761 agcgatcgat caagcgggta tccagtttag gatactaaac gcaagcaaag gaccggcggt
     5821 tcgcgctacc cgagctcagg cggatcgtgt gctctaccgt caggcggtac gtacggcgct
     5881 ggagaaccaa ccgaacctga tgatcttcca gcaggcggtt gaagatctta ttgtcgaaaa
     5941 cgatcgcgtg gtcggtgctg ttacccaaat gggactgaag ttccgtgcca aagcagtcgt
     6001 gctcaccgtt gggacgttcc tcgacggtaa aattcatatc ggtctggata attacagcgg
     6061 tggccgtgct ggtgatccgc cgtccattcc gctttctcgc cgtttgcgtg aactgccgct
     6121 gcgcgttggt cgtctgaaaa ccgggacacc accgcgtatt gatgctcgaa ctatcgactt
     6181 tagtgtgctg gcgcaacagc atggcgataa cccaatgccg gtattctcgt ttatgggcaa
     6241 tgcgtcccag catccccagc aggtgccgtg ttatatcact cataccaacg agaaaaccca
     6301 tgatgtgatc cgcagtaacc tcgatcgtag cccaatgtat gcaggggtga tcgaaggtgt
     6361 cggcccacgc tactgcccgt cgatcgaaga caaagtcatg cgcttcgccg acagaaatca
     6421 gcatcagatc ttccttgaac cggaaggact gacctctaat gaaatttatc cgaacggtat
     6481 ctccaccagc ctgccgttcg atgtgcagat gcaaatcgtc cgctctatgc aggggatgga
     6541 aaacgcgaag atcgtgcgtc cgggttatgc cattgagtat gacttcttcg atcctcgcga
     6601 cctgaaaccg acgctggaga gcaagtttat ccaggggctg ttctttgctg gtcagattaa
     6661 cggcactacc ggttacgaag aagccgctgc gcaaggtttg ctggccggtc ttaacgctgc
     6721 ccgtctgtct gctgacaaag aaggttgggc tccggcacgt tctcaggcgt atctcggcgt
     6781 actagttgat gacctgtgca ctttaggaac caaagaaccg tatcgtatgt ttacttcgcg
     6841 cgcagaatat cgtctgatgc tacgcgaaga taatgcggat ctgcgtttga ctgaaatcgg
     6901 tcgtgaactg ggcctggtgg atgacgaacg ttgggcgcgc tttaacgaga aacttgagaa
     6961 tatcgagcgt gagcgtcagc gtctgaaatc gacctgggta accccgtcgg cggaagctgc
     7021 agccgaagtg aatgctcacc tgactgcgcc gctttcccgt gaagccagtg gtgaagatct
     7081 gctgcgtcgt ccggaaatga cttatgaaaa attaaccacg ctgacgccgt ttgcccctgc
     7141 gttgacagac gaacaggcgg cggaacaggt tgagattcag gttaaatacg aaggttatat
     7201 cgcgcgccag caagatgaga tcgaaaagca gctgcgtaac gagaacaccc tgctacccgc
     7261 gacactggat taccgccagg tatccggtct ttctaacgaa gtgatcgcca aacttaacga
     7321 tcacaaacca gcctctatcg gccaagcttc gcgtatttct ggcgtcacgc ctgcggctat
     7381 ctccattctg ctggtgtggc tgaaaaaaca gggtatgctg cgtcgtagcg cataacgcat
     7441 taaaaatgcc tggtaagcac ccgcttacca ggcaacgcat caagaacagg taatcaccgt
     7501 gctcaacaaa ctctccttac tgctgaaaga cgcaggtatt tcgcttaccg atcaccagaa
     7561 aaaccagctt attgcctacg tgaatatgct gcataaatgg aacaaagcgt acaacctgac
     7621 ttcggtccgc gatcctaatg agatgctggt acgccatatt ctcgatagca ttgtggtggc
     7681 accgtatctg caaggtgaac ggtttatcga tgtcggcacc ggaccaggac tgccaggcat
     7741 tccactctct atcgtgcgtc ctgaagccca tttcactctg ttggatagcc ttggtaaacg
     7801 cgtgcgtttc cttcgtcagg tgcaacatga gcttaaactg gagaatattg aacctgtaca
     7861 gagcagggta gaagagtttc cttcagagcc gccatttgat ggcgtaatta gccgcgcttt
     7921 tgcctctctg aacgatatgg tgagctggtg ccaccatctt cctggtgagc aaggccgttt
     7981 ctacgcgctg aaagggcaaa tgccggaaga tgaaatcgct ttgttgcccg aagaatatca
     8041 ggtcgaatca gtggttaaac ttcaggttcc agccctggat ggcgaacgtc atctggtggt
     8101 gattaaagca aataaaattt aatttttatc aaaaaaatca taaaaaattg accggttaga
     8161 ctgttaacaa caaccaggtt ttctactgat ataactggtt acatttaacg ccacgttcac
     8221 tcttttgcat caacaagata acgtggcttt ttttggtaag cagaaaataa gtcattagtg
     8281 aaaatatcag tctgctaaaa atcggcgcta agaaccatca ttggctgcta aaacattatt
     8341 aaaaatgtca atgggtggtt tttgttgtgt aaatgtcatt tattaaaaca gtatctgttt
     8401 ttagactgaa atatcataaa cttgcaaagg catcatttgc caagtaaata aatatgctgt
     8461 gcgcgaacat gcgcaatatg tgatctgaag cacgctttat caccagtgtt tacgcgttat
     8521 ttacagtttt tcatgatcga acagggttag tagaaaagtc gcaattgtat gcactggaaa
     8581 aatatttaaa catttattca ccttttggct acttattgtt tgaaatcacg ggggcgcgcc
     8641 gtataatttg accgcttttt gatgcttgac tctaagcctt aaagaaagtt ttatacgaca
     8701 cgcggcatac ctcgaaggga gcaggagtaa aaaacgtgat gtctgtgtcg ctcgtgagtc
     8761 gaaacgttgc tcggaagctt ctgctcgttc agttactggt ggtgatagca agtggattgc
     8821 tgttcagcct caaagacccc ttctggggcg tctctgcaat aagcgggggc ctggcagtct
     8881 ttctgcctaa cgttttgttt atgatatttg cctggcgtca ccaggcgcat acaccagcga
     8941 aaggccgggt ggcctggaca ttcgcatttg gcgaagcttt caaagttctg gcgatgttgg
     9001 tgttactggt ggtggcgttg gcggttttaa aggcggtatt cttgccgctg atcgttacgt
     9061 gggttttggt gctggtggtt cagatactgg caccggctgt aattaacaac aaagggtaaa
     9121 aggcatcatg gcttcagaaa atatgacgcc gcaggattac ataggacacc acctgaataa
     9181 ccttcagctg gacctgcgta cattctcgct ggtggatcca caaaaccccc cagccacctt
     9241 ctggacaatc aatattgact ccatgttctt ctcggtggtg ctgggtctgt tgttcctggt
     9301 tttattccgt agcgtagcca aaaaggcgac cagcggtgtg ccaggtaagt ttcagaccgc
     9361 gattgagctg gtgatcggct ttgttaatgg tagcgtgaaa gacatgtacc atggcaaaag
     9421 caagctgatt gctccgctgg ccctgacgat cttcgtctgg gtattcctga tgaacctgat
     9481 ggatttactg cctatcgacc tgctgccgta cattgctgaa catgtactgg gtctgcctgc
     9541 actgcgtgtg gttccgtctg cggacgtgaa cgtaacgctg tctatggcat tgggcgtatt
     9601 tatcctgatt ctgttctaca gcatcaaaat gaaaggcatc ggcggcttca cgaaagagtt
     9661 gacgctgcag ccgttcaatc actgggcgtt cattcctgtc aacttaatcc ttgaaggggt
     9721 aagcctgctg tccaaaccag tttcactcgg tttgcgactg ttcggtaaca tgtatgccgg
     9781 tgagctgatt ttcattctga ttgctggtct gttgccgtgg tggtcacagt ggatcctgaa
     9841 tgtgccgtgg gccattttcc acatcctgat cattacgctg caagccttca tcttcatggt
     9901 tctgacgatc gtctatctgt cgatggcgtc tgaagaacat taatttacca acactactac
     9961 gttttaactg aaacaaactg gagactgtca tggaaaacct gaatatggat ctgctgtaca
    10021 tggctgccgc tgtgatgatg ggtctggcgg caatcggtgc tgcgatcggt atcggcatcc
    10081 tcgggggtaa attcttggaa ggcgcagcgc gtcaacctga tctgattcct ctgctgcgta
    10141 ctcagttctt tatcgttatg ggtctggtgg atgctatccc gatgatcgct gtaggtctgg
    10201 gtctgtacgt gatgttcgct gtcgcgtagt aagcgttgct tttatttaaa gagcaatatc
    10261 agaacgttaa ctaaatagag gcattgtgct gtgaatctta acgcaacaat cctcggccag
    10321 gccatcgcgt ttgtcctgtt cgttctgttc tgcatgaagt acgtatggcc gccattaatg
    10381 gcagccatcg aaaaacgtca aaaagaaatt gctgacggcc ttgcttccgc agaacgagca
    10441 cataaggacc ttgaccttgc aaaggccagc gcgaccgacc agctgaaaaa agcgaaagcg
    10501 gaagcccagg taatcatcga gcaggcgaac aaacgccgct cgcagattct ggacgaagcg
    10561 aaagctgagg cagaacagga acgtactaaa atcgtggccc aggcgcaggc agaaattgaa
    10621 gccgagcgta aacgtgcccg tgaagagctg cgtaagcaag ttgctatcct ggctgttgct
    10681 ggcgccgaga agatcatcga acgttccgtg gatgaagctg ctaacagcga catcgtggat
    10741 aaacttgtcg ctgaactgta aggagggagg ggctgatgtc tgaatttatt acggtagctc
    10801 gcccctacgc caaagcagct tttgactttg ccgtcgaaca ccaaagtgta gaacgctggc
    10861 aggacatgct ggcgtttgcc gccgaggtaa ccaaaaacga acaaatggca gagcttctct
    10921 ctggcgcgct tgcgccagaa acgctcgccg agtcgtttat cgcagtttgt ggtgagcaac
    10981 tggacgaaaa cggtcagaac ctgattcggg taatggctga aaatggtcgt cttaacgcgc
    11041 tcccggatgt tctggagcag tttattcacc ttcgtgccgt gagtgaggct accgctgagg
    11101 tagacgtcat ttccgctgcc gcactgagtg aacaacagct cgcgaaaatt tctgctgcga
    11161 tggaaaaacg tctgtcacgc aaagttaagc tgaattgcaa aatcgataag tctgtaatgg
    11221 caggcgttat catccgagcg ggtgatatgg tcattgatgg cagcgtacgc ggtcgtcttg
    11281 agcgccttgc agacgtcttg cagtcttaag gggactggag catgcaactg aattccaccg
    11341 aaatcagcga actgatcaag ctgcgcattg ctcagttcaa tgttgtgagt gaagctcaca
    11401 acgaaggtac tattgtttct gtaagtgacg gtgttatccg cattcacggc ctggccgatt
    11461 gtatgcaggg tgaaatgatc tccctgccgg gtaaccgtta cgctatcgca ctgaacctcg
    11521 agcgcgactc tgtaggtgcg gttgttatgg gtccgtacgc tgaccttgcc gaaggcatga
    11581 aagttaagtg tactggccgt atcctggaag ttccggttgg ccgtggcctg ctgggccgtg
    11641 tggttaacac tctgggtgca ccaatcgacg gtaaaggtcc gctggatcac gacggcttct
    11701 ctgctgtaga agcaatcgct ccgggcgtta tcgaacgtca gtccgtagat cagccggtac
    11761 agaccggtta taaagccgtt gactccatga tcccaatcgg tcgtggtcag cgtgaattga
    11821 tcatcggtga ccgtcagaca ggtaaaaccg cactggctat cgatgccatc atcaaccagc
    11881 gcgattccgg tatcaaatgt atctatgtcg ctatcggcca gaaagcgtcc accatttcta
    11941 acgtggtacg taaactggaa gagcacggcg cactggctaa caccatcgtt gtggtagcaa
    12001 ccgcgtctga atccgctgca ctgcaatacc tggcaccgta tgccggttgc gcaatgggcg
    12061 aatacttccg tgaccgcggt gaagatgcgc tgatcattta cgatgacctg tctaaacagg
    12121 ctgttgctta ccgtcagatc tccctgctgc tccgtcgtcc gccaggacgt gaagcattcc
    12181 cgggcgacgt tttctacctc cactctcgtc tgctggagcg tgctgcacgt gttaacgccg
    12241 aatacgttga agccttcacc aaaggtgaag tgaaagggaa aaccggttct ctgaccgcac
    12301 tgccgattat cgaaactcag gcgggtgacg tttctgcgtt cgttccgacc aacgtaatct
    12361 ccattaccga tggtcagatc ttcctggaaa ccaacctgtt caacgccggt attcgtcctg
    12421 cggttaaccc gggtatttcc gtatcccgtg ttggtggtgc agcacagacc aagatcatga
    12481 aaaaactgtc cggtggtatc cgtaccgctc tggcacagta tcgtgaactg gcagcgttct
    12541 ctcagtttgc atccgacctt gacgatgcaa cacgtaagca gcttgaccac ggtcagaaag
    12601 tgaccgaact gctgaaacag aaacagtatg cgccgatgtc cgttgcgcag cagtctctgg
    12661 ttctgttcgc agcagaacgt ggttacctgg cggatgttga actgtcgaaa attggcagct
    12721 tcgaagccgc tctgctggct tacgtcgacc gtgatcacgc tccgttgatg caagagatca
    12781 accagaccgg tggctacaac gacgaaatcg aaggcaaact gaaaggcatc ctcgattcct
    12841 tcaaagcaac ccaatcctgg taacgtctgg cggcttgcct tagggcaggc cgcaaggcat
    12901 tgaggagaag ctcatggccg gcgcaaaaga gatacgtagt aagatcgcaa gcgtccagaa
    12961 cacgcaaaag atcactaaag cgatggagat ggtcgccgct tccaaaatgc gtaaatcgca
    13021 ggatcgcatg gcggccagcc gtccttatgc agaaaccatg cgcaaagtga ttggtcacct
    13081 tgcacatggt aatctggaat ataagcacct ttacctggaa gaccgcgacg ttaaacgcgt
    13141 gggctacctg gtggtgtcga ccgaccgtgg tttgtgcggt ggtttgaaca ttaacctgtt
    13201 caaaaaactg ctggcggaaa tgaagacctg gaccgacaaa ggcgttcaat gcgacctcgc
    13261 aatgatcggc tcgaaaggcg tgtcgttctt caactccgtg ggcggcaatg ttgttgccca
    13321 ggtcaccggc atgggggata acccttccct gtccgaactg atcggtccgg taaaagtgat
    13381 gttgcaggcc tacgacgaag gccgtctgga caagctttac attgtcagca acaaatttat
    13441 taacaccatg tctcaggttc cgaccatcag ccagctgctg ccgttaccgg catcagatga
    13501 tgatgatctg aaacataaat cctgggatta cctgtacgaa cccgatccga aggcgttgct
    13561 ggataccctg ctgcgtcgtt atgtcgaatc tcaggtttat cagggcgtgg ttgaaaacct
    13621 ggccagcgag caggccgccc gtatggtggc gatgaaagcc gcgaccgaca atggcggcag
    13681 cctgattaaa gagctgcagt tggtatacaa caaagctcgt caggccagca ttactcagga
    13741 actcaccgag atcgtctcgg gggccgccgc ggtttaaaca ggttatttcg tagaggattt
    13801 aagatggcta ctggaaagat tgtccaggta atcggcgccg tagttgacgt cgaattccct
    13861 caggatgccg taccgcgcgt gtacgatgct cttgaggtgc aaaatggtaa tgagcgtctg
    13921 gtgctggaag ttcagcagca gctcggcggc ggtatcgtgc gtaccatcgc aatgggttcc
    13981 tccgacggtc tgcgtcgcgg tctggatgta aaagacctcg aacacccgat cgaagtcccg
    14041 gtaggtaaag cgactctggg ccgtatcatg aacgtactgg gtgaaccggt cgacatgaaa
    14101 ggcgagatcg gtgaagaaga gcgttgggcg attcaccgcg cagcaccttc ctacgaagag
    14161 ctgtcaaact ctcaggaact gctggaaacc ggtatcaaag ttatcgacct gatgtgtccg
    14221 ttcgctaagg gcggtaaagt tggtctgttc ggtggtgcgg gtgtaggtaa aaccgtaaac
    14281 atgatggagc tcattcgtaa catcgcgatc gagcactccg gttactctgt gtttgcgggc
    14341 gtaggtgaac gtactcgtga gggtaacgac ttctaccacg aaatgaccga ctccaacgtt
    14401 atcgacaaag tatccctggt gtatggccag atgaacgagc cgccgggaaa ccgtctgcgc
    14461 gttgctctga ccggtctgac catggctgag aaattccgtg acgaaggtcg tgacgttctg
    14521 ctgttcgttg acaacatcta tcgttacacc ctggccggta cggaagtatc cgcactgctg
    14581 ggccgtatgc cttcagcggt aggttatcag ccgaccctgg cggaagagat gggcgttctg
    14641 caggaacgta tcacctccac caaaactggt tctatcacct ccgtacaggc ggtatacgta
    14701 cctgcggatg acttgactga cccgtctccg gcaaccacct ttgcgcacct tgacgcaacc
    14761 gtggtactga gccgtcagat cgcgtctctg ggtatctacc cggccgttga cccgctggac
    14821 tccaccagcc gtcagctgga cccgctggtg gttggtcagg aacactacga caccgcgcgt
    14881 ggcgttcagt ccatcctgca acgttatcag gaactgaaag acatcatcgc catcctgggt
    14941 atggatgaac tgtctgaaga agacaaactg gtggtagcgc gtgctcgtaa gatccagcgc
    15001 ttcctgtccc agccgttctt cgtggcagaa gtattcaccg gttctccggg taaatacgtc
    15061 tccctgaaag acaccatccg tggctttaaa ggcatcatgg aaggcgaata cgatcacctg
    15121 ccggagcagg cgttctacat ggtcggttcc atcgaagaag ctgtggaaaa agccaaaaaa
    15181 ctttaacgcc ttaatcggag ggtgatatgg caatgactta ccacctggac gtcgtcagcg
    15241 cagagcaaca aatgttctct ggtctggtcg agaaaatcca ggtaacgggt agcgaaggtg
    15301 aactggggat ctaccctggc cacgcaccgc tgctcaccgc cattaagcct ggtatgattc
    15361 gcatcgtgaa acagcacggt cacgaagagt ttatctatct gtctggcggc attcttgaag
    15421 tgcagtctgg caacgtgacc gttctggccg acaccgcaat tcgcggccag gatctcgacg
    15481 aagcgcgagc catggaagcg aaacgtaagg ctgaagagca cattagcagc tctcacggcg
    15541 acgtagatta cgctcaggcg tctgcggaac tggccaaagc gatcgcgcag ctgcgcgtta
    15601 tcgagttgac caaaaaagcg atgtaacacc ggcttgaaaa gcacaaaagc cagtctgga
//
LOCUS       DAJTOR010000089        15634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTOR010000089 DAJTOR010000000
VERSION     DAJTOR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.089"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            337..429
                     /locus_tag="OO249_002479"
                     /pseudo
     CDS             337..429
                     /locus_tag="OO249_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            433..612
                     /locus_tag="OO249_002480"
                     /pseudo
     CDS             433..612
                     /locus_tag="OO249_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            946..3225
                     /gene="ydeP"
                     /locus_tag="OO249_002481"
     CDS             946..3225
                     /gene="ydeP"
                     /locus_tag="OO249_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5636741.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHTASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIEHISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3473..3670
                     /gene="safA"
                     /locus_tag="OO249_002482"
     CDS             3473..3670
                     /gene="safA"
                     /locus_tag="OO249_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5636742.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3745..4506
                     /gene="ydeO"
                     /locus_tag="OO249_002483"
     CDS             3745..4506
                     /gene="ydeO"
                     /locus_tag="OO249_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5636743.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            4908..6590
                     /locus_tag="OO249_002484"
     CDS             4908..6590
                     /locus_tag="OO249_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5636744.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGTVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYNLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6642..7799
                     /locus_tag="OO249_002485"
     CDS             6642..7799
                     /locus_tag="OO249_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5636745.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7765..7887)
                     /locus_tag="OO249_002486"
     CDS             complement(7765..7887)
                     /locus_tag="OO249_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636746.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8096..9781
                     /locus_tag="OO249_002487"
     CDS             8096..9781
                     /locus_tag="OO249_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR5636747.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            9819..12191
                     /locus_tag="OO249_002488"
     CDS             9819..12191
                     /locus_tag="OO249_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5636748.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVVGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12248..15031
                     /locus_tag="OO249_002489"
     CDS             12248..15031
                     /locus_tag="OO249_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5636749.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKKALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            15393..>15634
                     /locus_tag="OO249_002490"
     CDS             15393..>15634
                     /locus_tag="OO249_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5636750.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         4553 a         3398 c         3425 g         4258 t
ORIGIN      
        1 tggtgacggc agttgaaacc aatgtgttga ggttgcatgg acggttaagc cgagaatacg
       61 gtagtaagtg agatatgggg tttggtcgtt gttaatgagc aaccatgacc atgaccgcca
      121 acgactggat tctcccttgg tttatgttgg tgcgcataat gtatattatg ttaaatcata
      181 tctgagtgat gcgaagtgct tttctggtag tcgttattcg ttcagggttc cagaacttaa
      241 gcctctcctc tttcataatt ggcctgtgca tgacggatat aggctggctg gtatgcagat
      301 ttgccgtttg ccgcatcttc ccagcgttga agactcaagc tggcaaaccg cgtgcaaatg
      361 agcacgccct ctttccatca acacttcagg cagcttgcgg gaatgagtcc actacggtat
      421 cagaaatggg aagtgggtgt ctctctgatg cgtaatggaa aaatactggc tacgggagaa
      481 aatgtctccc tggggacagt aaataaaagc aaagtatctc tgggtttaag cgcaacctat
      541 ggacaaacag gaaacaaggt tgcagccgga accgtccagt cagtcattgg tgtcactttt
      601 atctatgaat aattgtgatt ttgatcttag tctatgcgct ccattcacga aaattgcgcg
      661 gataattatt ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa
      721 gccagagtct tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg
      781 cttaacttat cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt
      841 agtcccctac tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat
      901 cgctattaca aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt
      961 gaatcctacc agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta
     1021 cgtaagcaga tggatatacg ccaggatgtt attgccatgt ttgacatgaa taagccagag
     1081 ggctttgact gtccgggttg tgcatggcca gatcctaagc acactgcgtc attcgacatt
     1141 tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct
     1201 ttctttgctg agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct
     1261 gcgggacgtc tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta
     1321 agctggcaac aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat
     1381 caggttgaat tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt
     1441 tttgcccgtg aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg
     1501 accagtgtgg gtttggcagc gagtatcggc gtgggtaaag ggactgtgtt gctggaagac
     1561 tttgagaagt gcgatttagt catttgcatt gggcataacc ctggtacaaa ccacccccgc
     1621 atgctgactt cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct
     1681 ttacaggaac gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg
     1741 aactctgaga ctcagttggc cagtgcgtac tacaacgtgc gcattggtgg cgatatggcg
     1801 ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt
     1861 cggccctcat tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta
     1921 cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac atatttctgg actaagtcag
     1981 acacaaatcg ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac
     2041 ggaatgggga tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg
     2101 ctgttgatga aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac
     2161 tctaatgtac agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg
     2221 gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt
     2281 gccagcatgc aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac
     2341 tttgcactgg caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg
     2401 gcggtacacg tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat
     2461 attctgccgg ttctggggcg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta
     2521 accgttgagg attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt
     2581 gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccag
     2641 agcgtggtag cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa
     2701 gctgtgttac cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac
     2761 ctgataaatg cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt
     2821 accagcaaag ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg
     2881 acagtacgca gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga
     2941 ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt
     3001 gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca
     3061 cgccgcatgg atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc
     3121 tattttccag aatcgaatca catgctaact cttgataacc acgatccatt aagtggcatt
     3181 cctggttata aaagtattcc ggttgaatta gaaccatcaa attaattcgc ctcctcattt
     3241 cttctgctgt catccgcaca gcagaagaat ttctcattga ctattatcgc gcaatttgct
     3301 cacattgatc aaattaaacc acacactata agatataaac ctctgcctac agctgtaaga
     3361 aactccgctc agtactgaag tgccagtcct atttcctctt ttctccagcc tgttatatta
     3421 agcatactga ttaacaattt ttaacgttat ccgctaaata aacatatttg aaatgcatgc
     3481 gaccacagtg aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat
     3541 tgtggttgtc ttattactga cccttacgtt gatagccatt ttttcggcaa ttgatcagct
     3601 gggtatttca gaaatgggtc gcattgcaag agatcttaca catttcatta tcaatagttt
     3661 gcaagactga aaataaacag caaattataa atatgaaatg ttaaaaaagt atcgataaaa
     3721 actttattgt tttaaggaga taaaatgtcg ctcgtttgtt ctgttatatt tattcatcat
     3781 gccttcaacg ctaacatttt agataaagat tacgccttct ctgacggcga gatcctgatg
     3841 gtagataacg ctgttcgtac gcattttgaa ccttatgagc ggcattttaa agagatcgga
     3901 tttactgaaa ataccattaa aaaatatcta caatgcacta acatccagac agtgacggtg
     3961 cctgttcctg cgaagttttt acgtgcttca aatgtaccga ctggattgct taatgaaatg
     4021 attgcttatc tcaactcgga agaacgcaat catcataatt tttcagaact tttgcttttt
     4081 tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta cactattaac taacggtgtg
     4141 ctatccgttt ccggaaaagt gagaaatatt gtcaacatga agctggcgca cccatggaag
     4201 ctgaaagata tttgtgactg cctgtacatc agtgaaagcc tgttgaagaa aaaactaaag
     4261 caagagcaaa cgacattctc acagattcta ttagatgcca gaatgcagca cgcaaaaaat
     4321 ttgatacgcg tagaaggttc agtcaataaa attgccgaac aatgtggtta tgccagtact
     4381 tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca gtccgaagag agtttctaag
     4441 gagtaccgtt gtcaaagtca cacgggtatg aatacgggca acacgatgag tgctttagct
     4501 atttgattat ttgctaacga gtagtcaacc acgcacgcta cgtaagaatt atatggggca
     4561 gccattccct gccccgcgtt gtttttaggc gatatattta ttgaaataaa taagtgacat
     4621 ccatcacata tttatgcact tgcataacct gttgcatgat tatttatgtt ctcaattcag
     4681 catcttgtca gtaaaatgca ataatttatt aaatatcaat aaattagttg tttatcggcg
     4741 agaaattact taatagaaca gaaagtaatg tcaacgcttt atggactgtt atttcccttt
     4801 ttttagctaa atctgctatc tctttatgtg actaacttca cttacatcca cttatttctc
     4861 ttcgtaaaat tacttcggaa ttaagtacaa taagaagagg aacatttatg aagtctgcat
     4921 taaagaaaag tgtcgtaagt acctcgatat ctttgatact ggcatctggt atggctgcat
     4981 ttgctgctca tgcggcagat gatgtaaagc tgaaagcaac caaaacaaac gttgctttct
     5041 cagactttac gccgactgaa tacagtacca aaggaaagcc aaatattatc gtactaacca
     5101 tggatgatct tggttatgga caacttcctt ttgataaggg atcttttgac ccaaaaacaa
     5161 tggaaaatcg tgaagttgtc gatacctaca aaatagggat agataaagcc attgaagctg
     5221 cacaaaaatc aacgccgacg ctcctttcat taatggatga aggcgtacgt tttactaacg
     5281 gctatgtggc acacggtgtt tccggcccct cccgtgccgc aataatgacc ggtcgagctc
     5341 ccgcccgctt tggtgtctat tccaataccg atgctcagga tggtattccg ctaacagaaa
     5401 ctttcttgcc tgaattattc cagaatcatg gttattacac tgcagcagta ggtaaatggc
     5461 acttgtcaaa aatcagtaat gtgccggtac cggaagataa acaaacgcgt gactatcatg
     5521 acaacttcac cacattttct gcggaagaat ggcaacctca aaaccgtggc tttgattact
     5581 ttatgggatt ccacgctgca ggaacggcat attacaactc cccttcattg ttcaaaaatc
     5641 gtgaacgtgt ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag gcaattggcg
     5701 ttgttgatcg tgccaaaaca cttgaccagc cttttatgct ttacctggct tataatgctc
     5761 cgcacctgcc aaatgataat cctgcaccgg atcaatatca gaagcaattt aataccggta
     5821 gtcaaacagc agataactac tacgcttccg tttattctgt tgatcagggt gtaaaacgca
     5881 ttctcgaaca actgaagaaa aacggacagt atgataatac tattattctc tttacctccg
     5941 ataatggtac ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa ggctataaga
     6001 gtcagaccta tcctggcggt actcacaccc caatgtttat gtggtggaaa ggaaaacttc
     6061 aacccggtaa ttatgacaag ctgatttccg caatggattt ctacccgaca gctcttgatg
     6121 cagccgatat cagcattcca aaagacctta agctggatgg cgtttccttg ctgccctggt
     6181 tgcaagataa gaaacaaggc gagccacata aaaatctgac ctggataacc tcttattctc
     6241 actggtttga cgaggaaaat attccattct gggataatta ccacaaattt gttcgccatc
     6301 agtcagacga ttacccgcat aaccccaaca ctgaggactt aagccaattc tcttatacgg
     6361 tgagaaataa cgattattcg cttgtctata cagtagaaaa caatcagtta ggtctctaca
     6421 atctgacgga tctacagcaa aaagataacc ttgccgccgc caatccgcag gtcgttaaag
     6481 agatgcaagg cgtggtaaga gagtttatcg acagcagcca gccaccgctt agcgaggtaa
     6541 atcaggagaa gtttaacaat atcaagaaag cactaagcga agcgaaataa ctaaaccttc
     6601 atgcggcgga attttccgcc gccttattga gcgagatagc gatgcacgtt acagccaagc
     6661 cctccagttt tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa aaagagtcgc
     6721 agtttactca tgaaaaatgg atggatgaca gcactctgaa agagttcatc aaacaatata
     6781 tcgcagcgtc tggcaatcag gtctatttta cctggcaagg cggtgaaccc actctggctg
     6841 gcctggattt tttccgtaaa gttattcact atcaacaacg ctatgcaggc caaaaacgta
     6901 tttttaatgc attacaaacg aatggcattt tattgaataa tgaatggtgt tcctttctca
     6961 aagaacatga atttctggtt ggtatctcga tcgatggccc ccaggagtta catgaccgtt
     7021 acagacgcag taattcaggt aacggtactt ttgcaaaagt gatagcagcc atcgagcgtc
     7081 tgaaatcata tcaagttgag tttaatacgt taaccgtcat taataacgtt aatgtccatt
     7141 accctcttga ggtttatcat tttttaaaat ctatcggcag taaacatatg caatttatcg
     7201 aattgctcga aaccgggacg ccgaatattg atttcagtgg tcatagtgag aacacattcc
     7261 gtatcattga tttttctgtg cctcccacgg cttatggcaa gtttatgtca accattttta
     7321 tgcaatgggt taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa agctttgtca
     7381 gccgtttttt ggggaatggg catacaagtt gtattttcca ggagtcctgc aaggataatc
     7441 tggttgttga aagtaatgga gacatttacg aatgcgacca ttttgtctat ccacagtaca
     7501 aaattggaaa cattaataaa tctgaactca aaacgatgaa cagtgtacaa ctgacagcgc
     7561 aaaaaaaacg gatttcagcg aaatgtcagc aatgtgcata taaacctatc tgcaatggcg
     7621 gttgtcctaa gcatcgtatt actaaagtaa acaatgagac tgtttcttat ttttgcgaag
     7681 gttataaaat ccttttttca accatggtac cttatatgaa cgccatggtt gagttagcta
     7741 agaacagagt accgctttac cacattatgg atgttgcaaa acaaatggag aataattaat
     7801 aacgctctct tttaactcca ttcttagcct ccttcttatg accttaacaa ggcacccgac
     7861 ataaattcct gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc ctggcttacg
     7921 tggtatgcgt cagggcatta gcaataccct tgattatcaa tagcataacc ataaccataa
     7981 ccactgaaaa ttaaaaattt ctatatattt catattgtta aatgatactt aaataaattt
     8041 catcagtaaa tcattgccat agataatcat tggatggcac aatcaatttt cgctaatgat
     8101 aaccattccc attacgtttt gtatgttgat agcaaagtat ctctgtctgc tgaagccctt
     8161 ttggctgcgt aagaataata aaacttcagt gttgttaatc attattatcc tcgcaatgat
     8221 ccttggcgtg gttaaaattc aggtctggtt aaacgactgg aataatgatt ttttcaatgc
     8281 gctaagccag aaagaaaccg ataagctttg gcagcttgtt ctttggttcc ctgccctgct
     8341 ggggatcttt gtattaatat ccgtaaacaa aacatggtta attaaactac tgactattcg
     8401 ctggcgcgaa tggttaacag attactacct taatcgctgg tttgcagata aaaactacta
     8461 cttcacgcag atctacggcg agcataaaaa tacagataac cctgaccaac gtatcgctga
     8521 agacattctc ttactgatca gcaaaacgct cagtctttca tttggcttca tccagtcact
     8581 tagcatgctg atcaccttta ccgttattct ttggcaaagc gcgggtacgc tctcattcac
     8641 tgttggtgga acagaatgga atatccaggg gtatatggtc tataccgtcg tgctcattgt
     8701 gatcggtgga actttattta cccataaggt tggtaagcgt attcgtccgc ttaatgtgga
     8761 aaaacaacgt agcgaagcga catttcgaac taatcttgtg cagcataata agcaggcaga
     8821 actgattgcg ctaagcaatg ctgagtctct acaacgccag gagttgcgcg acaattttca
     8881 taccattaaa gagaactggc atcgtttaat gaatcgtcag cggtggcttg attactggca
     8941 aaatatttat tcgcgctcgc ttagcgttct tccctacttt ctgttattgc cgcagtttat
     9001 tagcgggcag atcaatctgg gcggactgat gaaatcgcgc caggcattta tgctagtatc
     9061 gaacaattta agctggttta tttataaata tgacgaactt gctgaactgg ctgcggttat
     9121 cgatcgcttg tatgagttcc atcaactcac tgaacagcgc cctacgaata agcctaaaaa
     9181 ttgccaacat gcggtacaag tggctaatgc gagtattcgt acgcctgata ataagatcat
     9241 attagagaac ttgaactttc atgtttcgcc aggcaaatgg ctattactga aaggctactc
     9301 tggtgcggga aaaaccacac tgcttaaaac attatcccac tgctggccgt ggtttaaagg
     9361 tgatattttt tctcctgctg acagttggta tgtgtcacaa acaccgttaa tcaaaaccgg
     9421 cttactgaaa gagattattt gtaaagcact tcccctgccc gtagacgata aatcgttgag
     9481 cgaagtactg catcaggttg gtcttgggaa attggctgcg cgtattcatg accacgatcg
     9541 ctggggagat attctttcca gcggcgaaaa acaacgtatc gccctggcac gattaatttt
     9601 acgacgtccg aaatggatat ttcttgacga aactacctct catcttgagg aacaagaggc
     9661 tatccgctta ctgcgtttag tgcgtgaaaa actacccaca agcggcgtca ttatggttac
     9721 acatcaaccc ggtgtctgga acctggccga tgatatttgt gacattagcg cggttttata
     9781 aaaacaataa aagccctgct gtacggagat ataaataaat gaagcgagtt cttattcctg
     9841 gcgtcatttt atgtggcgct gatgtggcgc aggccgtcga tgacaaaaac atgtacatgc
     9901 atttttttga agagatgacg gtctatgctc ctgtccctgt acccgtaaac ggcaacacgc
     9961 attacaccag tgaaagcatc gagcgtttac cgaccgggaa tggcaatatc agcgatctgc
    10021 tgagaaccaa ccctgcggta cgcatggatt caacgcaaag tacctcgttg aaccagggag
    10081 atattcgccc tgagaaaatc tctattcacg gtgcgtcgcc ctaccagaat gcctatttga
    10141 ttgacggtat tagtgcaact aataacctga acccagcgaa tgagtccgat gccagtagtg
    10201 caaccaatat tagcgggatg tcacaggggt attatcttga tgtcagctta ctggacaatg
    10261 tgacgcttta tgacagtttt gtgccggttg aatttggtcg cttcaatggc ggggtaattg
    10321 atgcaaagat caaacgcttc aacgctgatg atagcaaggt gaaattgggt tatcgcacta
    10381 cgcgttcgga ctggttaaca tcgcatatcg atgagaataa caagagcgca tttaatcaag
    10441 gttcttcagg aagtacttat ttctctccag attttaaaaa gaacttttat accttgtcgt
    10501 ttaatcagga gctggctgat aactttggcg ttaccgccgg tttatcgcgc cgccagtctg
    10561 atatcacccg cgcggattat gtttcgaatg acggcattgt cgtcggtcgg gcacagtata
    10621 aaaacgttat cgatactgca ttgagcaaat ttacctggtt tgccagcgac cgctttaccc
    10681 acgatttaac cttaaaatat accggctcca gccgtgatta taataccagc accttcccgc
    10741 agtctgatcg cgaaatgggt aataaatcct atggtcttgc atgggatatg gatacgcaac
    10801 tcgcatgggc caaactacgt accaccgttg gttgggatca tattagtgat tatacccgtc
    10861 acgatcatga catctggtac accgaacttt catgtacata tggtgatatt acagggcgtt
    10921 gtacccgtgg cggattagga cacatttccc aggctgtaga taattacacc ttcaaaacac
    10981 gcctggactg gcaaaaattc gccgtgggtg atgtttcgca tcaaccctac tccggcgcgg
    11041 aatacatcta ttccgatgca tggactgaac gccataacca gtctgaatcc tatgtgatta
    11101 atgctgcagg aaagaaaact aaccatacca tttaccataa aggtaaaggc agcctaggaa
    11161 ttgacaacta cacgctgtat atggcggatc acattagctg gcggaatgtg tcgttaatgc
    11221 ccggtgtgcg ttatgactat gacaactatc tgtcaaacca caatatctcc ccgcgcttta
    11281 tgacggaatg ggatattttt gctgatcaaa cctcaatgat taccgccggt tataaccgtt
    11341 actatggcgg gaatattctt gatatgggat tacgtgatat ccgcaatagc tggacggaat
    11401 cggtatcagg taataaaacc ctgacgcgtt atcagaattt gaaaacgcct tataacgatg
    11461 aactggcaat gggattgcag caaaaaatcg gtaagaacgt tattgcacgc gcaaactatg
    11521 tttaccgtga agcgcatgat caaatcagca aaagcagtcg taccgacagc gcgactaaaa
    11581 ccaccattac tgaatataac aacgatggca aaaccaaaac gcattcgttt aacctcagtt
    11641 ttgaactggc cgaacccctg catatccgcc aggtagatat taacccgcaa attgtcttta
    11701 gctatatcaa gagcaagggc aacttgtcgt taaacaatgg ttatgaggag agcaataccg
    11761 gtgataacca ggtggtttat aacggtaatc tggtctctta cgatagcgtt ccagtggcag
    11821 attttaataa cccattaaag atctccttaa acatggattt cacgcatcaa ccgagcgggt
    11881 tagtgtgggc gaatacgctg gcgtggcaag aagcgcgtaa agctcgcatt atccttggta
    11941 agacaaatgc gcaatacatc agcgaatatt cagattacaa gcagtatgtt gacgaaaaac
    12001 tggatagcag cctgacctgg gacacccgct tgtcctggac gccacaattt ctgaaacaac
    12061 aaaacctgac gatcagtgcc gatattctca atgtactgga tagcaaaacc gctgttgata
    12121 caacgaatac cggtgtggcg acctacgcca gtggccgtac tttctggctt gatgtcagca
    12181 tgaaatttta atagttcgga ttttacttaa ccgggtaaat tcgcccggtt ttcgcatgga
    12241 gataattatg agaaacctct gtttcttact gacgttagtg gcaactctgt tgctccccgg
    12301 gcggctgatt gccgccgcct taccgcagga tgaaaagtta attaccgggc aactggacaa
    12361 tggcttgcga tatatgattt atccgcatgc tcaaccaaag gatcaggtaa atttatggct
    12421 gcaaattcat accggttcat tgcaggaaga agacaatgag cgcggcgtgg ctcattttgt
    12481 agaacatatg atgtttaacg gcacaaaaac atggccgggt aataaagtca tcgaaacatt
    12541 tgagtcaatg ggcctgcgtt ttggtcgcga tgttaatgcc tataccagct atgacgaaac
    12601 ggtgtatcag gtgagtttgc cgactacgca gaaacaaaat ctgcaacaag tgatggcaat
    12661 cttcagtgaa tggagtaatg ccgcaacctt tgaaaaactc gaagtagacg ctgaacgtgg
    12721 cgtaattact gaggaatggc gtgcccatca ggatgcgaaa tggcgcacct ctcaggcgcg
    12781 ccgccctttc ctgctggcta atacccgtaa tttagaccgt gaacctatcg gcctgatgga
    12841 tactgtcgcc acggtcacac cggcacaatt gcgccaattt tatcaacgct ggtatcaacc
    12901 aaataatatg acctttatcg tggtcggcga tatcgacagt aaaaaagcgc tggcgctgat
    12961 aaaggataat ttaagtaagc ttccggctaa caaagcagct gaaaatcgcg tctggccgac
    13021 aaaagccgaa aaccacctgc gctttaatat cattaatgat aaagaaaacc gggtgaacgg
    13081 catcgcactc tattatcgcc tgccaatggt acaagtgaac gatgagcaaa gctttatcga
    13141 acaagctgaa tggagcatgt tagttcagct gttcaatcaa cgtctgcagg aacgcataca
    13201 gtcgggcgag ttgaagacta tttctggcgg cactgcgcgc agcgttaaaa ttgcacccga
    13261 ttatcagtcg ctgtttttcc gtgtaaatgc acgagacgat aatatgcagg atgctgcgaa
    13321 tgcattaatg gcagagttgg caaccattga tcagcatggt ttttctgctg aagaactcga
    13381 tgatgtcaaa tctacccgcc tcacctggct gaaaaatgcg gttgatcagc aagctgaacg
    13441 tgatttacgt atgctgacca gtcgcctggc atccagctca ttaaataata cgccgttctt
    13501 gtcgccggaa gagacatatc aactttcgaa acgtctgtgg cagcaaatta ccgtgcaaag
    13561 tctggcggaa aaatggcagc agttaagaaa gaaccaggac gcattttggg agcaaatggt
    13621 aaacaatgag cttgccgcca aaaaagcatt gtctcctgcg gctatcctgg cgctggaaaa
    13681 agagtacgcc aacaaaaagc tggcggctta catcttccca ggcagaaatt tatcgttaac
    13741 agtagacgct gacccacagg cggaaattag cagcaaagaa acgctggctg agaatctgac
    13801 atcattaaca ctttccaatg gtgccagggt tattctggca aaatccgcgg gtgaagagca
    13861 aaagctacaa attactgccg tatcgaataa aggcgattta agtttccctg cgcagcaaaa
    13921 atcacttatc gcgctggcaa ataaagcagt aagcggaagc ggcgttggtg agctctcctc
    13981 ttccagcctg aaacgctgga gtgcggaaaa ttcggtaacc atgagcagta aagtcagtgg
    14041 catgaatacg ttgctctccg ttagcgcgcg gactaataac cctgaacctg gttttcagtt
    14101 gattaaccag cgaatcaccc acagcacgat taacgacaat atttgggcat cgctacaaaa
    14161 tgctcaaatt caggcgttga aaacgctcga ccagcgtcca gcggagaaat tcgcccagca
    14221 gatgtatgag acgcgctatg ctgatgaccg cacgaaatta ctgcaagaaa atcagattgt
    14281 acagtttact gccgcagatg cgctggctgc cgatcgccag ttgttttcat ctccagcgga
    14341 tatcacgttt gtcattgtcg gtaatgtctc agaagacaaa ctcgtggcgt taattacgcg
    14401 ttacttagga tcaatcaaac actctgattc gccattagcc gcaggtaaac cattaactcg
    14461 cgcgacggac aacgcatcgg ttactgtaaa agaacaaaat gaacctgtgg cacaggtttc
    14521 acagtggaag cgttatgatt cccggacacc tgttaatctg gcgacgcgta tggcgctcga
    14581 tgcttttaac gtcgcactgg caaaagatct acgtgttaat attcgtgaac aggcatctgg
    14641 agcatacagc gtttcttctc gcctctcggt tgatcctcag gccaaagata tcagtcattt
    14701 gctggctttt acttgtcaac cagaacgaca tgatgaactg ttaacgttgg cgaatgaagt
    14761 gatggttaag cgtctggcta aagggatcag tgagcaagaa ctgaatgaat accagcaaaa
    14821 cgttcagcgc agcctcgata tccaacagcg tagcgttcaa caattagcga acactattgt
    14881 aaatagtctt attcaatatg acgatcctgc agcatggact gagcaggagc aattgttgaa
    14941 acaaatgacg gtagagaatg ttaacactgc cgttaaacaa tatctttctc atccggtaaa
    15001 tacttatacc ggagtattat tgccaaaata ataacagccc cgtcaacaca tcgttggcgg
    15061 ggattttagc aatattcgct atttttatgt aataatttta taaatgcgtt caaaataata
    15121 atcaagtact aatagtgata ttttaaggtc tgatttttac gtgataattc aggagacaca
    15181 gaatgcgcat aaaaataaca gcataaaaca ccttaccacc acccaagaat ttcatattgt
    15241 attgtttttc aatgaaaaaa cattattcgc gtaatatctc acgataaata acattaggat
    15301 tttgttattt aaacacgagt cctttgcact tgcttacttt atcgataaat cctacttttt
    15361 taatgcgatc caatcatttt aaggagttta aaatggataa gaagcaagta acggatttaa
    15421 ggtcggaact actcgattca cgttttggtg cgaagtctat ttccactatc gcagaatcaa
    15481 aacgttttcc gctgcacgaa atgcgcgacg atgtcgcatt ccagattatc aatgacgaat
    15541 tatatcttga tggcaacgct cgtcagaacc tggccacttt ctgccagacc tgggacgacg
    15601 aaaatgtcca caaattgatg gatttatcca ttaa
//
LOCUS       DAJTOR010000090        15445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTOR010000090 DAJTOR010000000
VERSION     DAJTOR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.090"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..447
                     /locus_tag="OO249_002491"
                     /pseudo
     CDS             1..447
                     /locus_tag="OO249_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490532.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            661..1809
                     /locus_tag="OO249_002492"
     CDS             661..1809
                     /locus_tag="OO249_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5636751.1"
                     /translation="MFEIDSLLLITSVIILSLLAVKLFDEISPIQLVDHGRNNQIDGM
                     RGFLAIFVLIHHAAIWHGYLLTGVWKTPSSNLLTNLGQVGVSFFFMITGYLFFSKIRS
                     SDQDWVRLYISRFLRLTPMFIVSLCLVLLVIGFKSRWSVHVSPASLTVSLMRWAPFTA
                     LGMPNINGVKDSFTINAAVTWTLVYEWFFYFSLPVIAALFKRRVSIHMIIISVLVLVI
                     FLCYPSKKIHVISFLFGLLASYSNNYVHRVAKAKITPIMIIALLSYEMIYFPTTYAPL
                     PLIICGVCFILIASGCDFYGILRLNLTRKLGETTYSIYLLHGVFLYCVMTWVIPSNYS
                     NYFFIMLISITAFCVTLLSCITFKLIELPFINITKQTAMKIRGIIKNT"
     gene            complement(2089..2700)
                     /locus_tag="OO249_002493"
     CDS             complement(2089..2700)
                     /locus_tag="OO249_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5636752.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAIGVGI
                     PAYSCLDAPGAYKAGYAICRSADFNSWEYVPDHRGEIVFSTETGESKEITAPGDYPDN
                     TTTIVPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDTAMASINLIQLKLQAG
                     RNLTQAETSRLNTVLDYIDAVTATDTSTAPDVIWPELLDAHNM"
     gene            complement(2700..3482)
                     /locus_tag="OO249_002494"
     CDS             complement(2700..3482)
                     /locus_tag="OO249_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR5636753.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEG
                     SALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDD
                     GRGVDNGRGLLTLQDGAIVSHNHYWGIWTSRTNDQTLESFTGTTILKQITPLSPSIDF
                     DNYPIPNPAITEGGVVAATTKPAGANETRPRNVAFNYIVRAA"
     gene            complement(3486..4070)
                     /locus_tag="OO249_002495"
     CDS             complement(3486..4070)
                     /locus_tag="OO249_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR5636754.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMQELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRFWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(4061..5119)
                     /locus_tag="OO249_002496"
     CDS             complement(4061..5119)
                     /locus_tag="OO249_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR5636755.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATVASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTTTDDAISSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NITIAKNELAVLGAISWT"
     gene            complement(5106..5531)
                     /locus_tag="OO249_002497"
     CDS             complement(5106..5531)
                     /locus_tag="OO249_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR5636756.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLRRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(5531..6079)
                     /locus_tag="OO249_002498"
     CDS             complement(5531..6079)
                     /locus_tag="OO249_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR5636757.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(6079..7158)
                     /locus_tag="OO249_002499"
     CDS             complement(6079..7158)
                     /locus_tag="OO249_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR5636758.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARKRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(7155..8483)
                     /locus_tag="OO249_002500"
     CDS             complement(7155..8483)
                     /locus_tag="OO249_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR5636759.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(8544..10379)
                     /locus_tag="OO249_002501"
     CDS             complement(8544..10379)
                     /locus_tag="OO249_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5636760.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYQAVDPAQSNNASGATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            complement(10521..10790)
                     /locus_tag="OO249_002502"
     CDS             complement(10521..10790)
                     /locus_tag="OO249_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5636761.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(10790..11146)
                     /locus_tag="OO249_002503"
     CDS             complement(10790..11146)
                     /locus_tag="OO249_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5636762.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNKTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(11146..12642)
                     /locus_tag="OO249_002504"
                     /pseudo
     CDS             complement(11146..12642)
                     /locus_tag="OO249_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
     gene            complement(12626..12796)
                     /locus_tag="OO249_002505"
     CDS             complement(12626..12796)
                     /locus_tag="OO249_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5636763.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(12823..13365)
                     /locus_tag="OO249_002506"
     CDS             complement(12823..13365)
                     /locus_tag="OO249_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636764.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHT"
     gene            complement(13362..13868)
                     /locus_tag="OO249_002507"
     CDS             complement(13362..13868)
                     /locus_tag="OO249_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636765.1"
                     /translation="MATPFFHVDVQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKKRAGFMTRIAPNQRNGKGNRMISGDFYP
                     AFLFFGVRGGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(13843..14253)
                     /locus_tag="OO249_002508"
     CDS             complement(13843..14253)
                     /locus_tag="OO249_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5636766.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCDDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGSNGDSLFSR"
     gene            complement(14250..14573)
                     /locus_tag="OO249_002509"
     CDS             complement(14250..14573)
                     /locus_tag="OO249_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5636767.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            complement(14576..14776)
                     /locus_tag="OO249_002510"
     CDS             complement(14576..14776)
                     /locus_tag="OO249_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636768.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            complement(14825..>15445)
                     /locus_tag="OO249_002511"
     CDS             complement(14825..>15445)
                     /locus_tag="OO249_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR5636769.1"
                     /translation="TQKMLDDAFFNVEAWINSELATEFAEQEEIAFTTGDGTKKPKGF
                     LANESTDETDKVRAFGKLQHIVSGDATAVTADAIIKLIYTLRKAHRTGAKFMMNNNSL
                     FAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQMPDIAADAKAIAFGNFKRGYT
                     IVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLLKIAVA"
BASE COUNT         3539 a         4393 c         3718 g         3795 t
ORIGIN      
        1 aaagggcaac agaaacgcat cgctacgccg ggacagaacc tgaagcatta tctggctggc
       61 gcactgcact caggtacggg acgggttcac taggccagtg gcagcagcaa aagttctgat
      121 ttatttatca gtctgttaga ggcattacgg cgtagatatc ggcgggcgaa aaccatcaca
      181 ctggtggcgg ataactacat catccataaa ggccgtaaag tggagcgatg gctggaagaa
      241 aacccgaagt tccggttgct gttcctgccg acgtattcac cgtggctgaa tccgatagag
      301 caactttggt tatcgttaca caagaccata acgcgaaatc atcagtgccg gtatatgtgg
      361 cagatactga aacaggtaac gcagtttatg aatgctgctt taccgtttcc gggtaatcaa
      421 cacggtatgg ccaaagtgga gcggtaatat gcgaagctat ttagattcag tttgaatctc
      481 agccctgaca ttcattcttt accgacgttc cattagccca aatctgagta tataacctca
      541 attcaactga ctaaatttaa cacaatatca atgacaaatg tctttaccat tggtacatac
      601 aattgcaaaa tcaccatctc taagcaataa gactacgcat attaaagtga gttatattaa
      661 atgtttgaaa ttgatagcct attattaata acatccgtga taatcttgtc attattggct
      721 gtaaaattat ttgatgaaat ttctcctata caacttgtcg atcatggaag aaataatcag
      781 atagatggaa tgagaggatt cttagccatc ttcgtactta ttcatcatgc cgctatttgg
      841 catggctact tattaaccgg ggtatggaaa actccgtcat caaacttatt aacaaattta
      901 ggccaagttg gtgtctcatt ctttttcatg atcaccggat atctattttt ttcgaagatt
      961 cgctcaagtg accaggattg ggtaagacta tatatatcga gatttctgcg attaactcct
     1021 atgtttattg taagtttatg tttagttctt cttgttatag gttttaaatc aagatggagc
     1081 gtacatgtct cccctgcctc tcttactgtg tcactcatga gatgggcacc attcaccgca
     1141 ttagggatgc caaatattaa cggtgttaag gactcattca ctatcaatgc agctgtaaca
     1201 tggaccctcg tttacgaatg gtttttttat ttttcattac cggttatagc tgcactcttc
     1261 aaaagaagag tcagcatcca catgattata ataagtgttt tagttctagt gattttctta
     1321 tgttacccat caaaaaaaat acatgttatt tcatttttat ttggactgtt agcctcttat
     1381 tcaaataatt atgtacatag agttgctaaa gctaaaataa cgcctattat gataattgca
     1441 ttactaagtt acgaaatgat atattttcct acaacctacg ctccactccc tctcataatt
     1501 tgcggtgttt gctttatact tatcgcatca ggctgtgatt tttatggtat attgagatta
     1561 aatttaacaa gaaaacttgg tgaaacaaca tatagtattt atcttttaca tggagtattc
     1621 ctttattgtg taatgacatg ggtcatccct agtaattatt ctaattattt ttttattatg
     1681 ttaataagca ttactgcttt ttgcgtcaca cttttatcat gcataacatt taaattaatt
     1741 gaactacctt tcattaatat aacaaaacaa accgctatga aaatcagggg cataataaaa
     1801 aacacttagt aatcaattgt cacctaatca agaggtggtt tatagataac aataaagaac
     1861 cttcgccatt tccggaggtt cttttatatc cttatggtaa atataaatta taaatattat
     1921 ttttatggtg ttccactatg aacagggaag ctaaataatg attacaaaaa tatgtaactt
     1981 caggcaatgc aggggctatg ttattgcctt aacaccataa tgagtaatat tgtgtcccga
     2041 ggataaaagt actgttaatt cttattgatg cttcaatagc actcaaaatc acatattatg
     2101 tgcatccaac aactcaggcc agatgacatc cggcgcggtg ctggtatctg ttgccgtcac
     2161 cgcgtcaatg taatccagca ccgtgttaag tcggctggtt tctgcctgcg tcagattccg
     2221 tccggcctgt aattttagtt gaatcagatt gatggaagcc attgcagtat caatcagcga
     2281 ctggcgttgt gcttctgctg catcgactgc ggcgctatgt tgtgcctcgg tatccgtcac
     2341 ccatttctca ccatcccatt tatcgtatgg tgttaacggg acgatagtgg ttgtattatc
     2401 agggtaatca cccggagctg tgatttcttt tgattctcct gtttcggtgc taaagacgat
     2461 ttcaccgcga tggtctggca catattccca tgagttaaaa tctgcagaac ggcagattgc
     2521 ataaccagct ttgtatgcgc ctggagcatc taaacaggaa tatgccggaa tgccgacacc
     2581 aatagcaaga tattcagttg aagctgaaat atactcccga ctcatgacat caaagttata
     2641 aacagtaatt tctcctgcca tagtggcaat taattcactg tttaattctg cgttattcat
     2701 tatgcagccc tcacaatata gttaaaagcg acatttcgtg ggcgtgtttc attcgcacct
     2761 gcaggtttag tcgttgccgc aacaacacca ccctctgtaa tagccgggtt gggaattggg
     2821 taattatcga agtcaatgga cggagacagg ggcgtgattt gttttaaaat cgtggtgcct
     2881 gtaaaacttt ccagagtctg gtcgttagtt cgtgaagtcc agattcccca atagtggtta
     2941 tgactgacaa tagcaccgtc ctgaagcgtt aataatcccc ttccgttatc cactccacgc
     3001 ccgtcatccc agccccgaat aaactcaccg cgtaaatcag gtaatttatt tgtcggataa
     3061 gcctttgcca gttccgggta ttcttcagca gaaaaagctg ctccgttgca tttcagccag
     3121 cctgttggcg gagtggctga aggccacgga acaggcacac caacgggtaa tgctgagcct
     3181 tctcccaaac caaggttttc gagagctgtt ttcaccgtgc catccgattt gatatcgcca
     3241 aacggattct tgcggcttaa cagcagcgca cgaagcgcgg taagcagctg gtcatgccgc
     3301 cccttctcca ggctggcacc ggatgcctcc acaacgctgc aaagctcctc ctgcaacatg
     3361 tcaaagtagt catcatccag atcggtggca ggcgtgccgg tctgggggtt accacgggta
     3421 aaaccgttct tacccgcgcc gaacttatcc ttctgcgcgg ttttcgtgtc tatacgatgc
     3481 atggattact ccggatattt aaaaatcaca taggtatgcg acgggcagag tttgttaaga
     3541 acacactcga caacggtgtc cccccagaag cgcagcgcgg aatcacaggg atcgccacat
     3601 gtcatccagg tggtgttggt ggcggctggc atgttgacct gccagtaata ccgccattcc
     3661 ggcgcattca ccgcgtcagt acaggcagat gagcaggtga acgtgctttt gtcgtatcgc
     3721 gtgatggtgg cgtctggtct gcccaaggca gcaagctgtg caaggtaaaa atcctcattg
     3781 atgccgcccg ccaggttaac cttcgcatcc agccgttgct gacgctggcg aagggtctgc
     3841 gtccctgccg gaatacattc atccggcagg ccgcacagac gctcccagcg gtttatcagt
     3901 tcggtggtgg tgcgcggatc cagctcctgc atcagggcat ccgcacgctg atgaacacgg
     3961 gttaatgacg gtgccgcacc ggcaatcgcc ggatcgctgg ctgaccacgc cggaccgggc
     4021 ggcaacagtg ctgacaacag acggatgtaa tcatcgtttg tcacgtccat gaaatcgccc
     4081 ccagaaccgc cagttcattt ttcgcaatgg tgatattgtc cgccggtgca agcaactgat
     4141 ggctgtattc cccgttcgca ccggaaatcg cttcactgat acgtgacacc ttcagttctc
     4201 cctgcggata accatcacgc agcaggaacg aacgcaactc cgcggtgatg gcagcccgta
     4261 tttccggtgt gtccggcgtc acacggatat gaaaatccac cgtatgtgcc accggcctga
     4321 acacatacaa atcagagcct gccaccgggg ccagtggccc gatatgttgt cttgccgccg
     4381 tttccgttga ttcttccgga atgggattaa tcaggtcact gctggcaatc atcacaccga
     4441 cagtccccgt tcccatccag tgtcggtatg tccatgcgcg ggtaatgcca ggcacttctt
     4501 tagcccagac gacatagtcc ccgtcagccc cgccctgcgg cgtccagtaa taccgctcaa
     4561 tgacgcgggc gcgccacgtt tccagctctt cagtatcaaa tccgcctgtc agggtgtcag
     4621 ccacaccgga agacggcaga ccattcaccg gcgtgaccag gattaatgcc gtaccgtcgt
     4681 cagcgttacc gaccgcacct gcacttgagc aggcgatcgg cacgcgcagg acaccaccgg
     4741 agctgattgc atcgtcagtt gtcgtgtact gcaccaggtc atcgcgctga ataacactcc
     4801 cggcagtcac cttcaggcca tcgctgacac cttcccagcg catatacccg ctggcaaccg
     4861 tggccccctt gcgcggacac cgtttcatcg cagcatgtcg cgccagccag gactcatcgc
     4921 acaggtcagg cagcatattc attgccagat aatcgatgta accgtagacc gtatgcagcg
     4981 ccgccgcata cacctttgcc cgcacgtctt catccatgcg ccggagcgtg tcgctgacgt
     5041 ccagcctggc gaataaatcg ttacggagca tactgatatt ttctgccagc gtcgggcgct
     5101 gaaattcact gtccgccatg cgttatcgca ctccacagat catcaaaaga aatcattact
     5161 ggtccatcac gacgcctgag ggtgatactg ttacccagct cattaatccc ggtgcggcgg
     5221 atatccagat caatacggga caccacgccg tcatcagtca tccattgcag gcattcgcgg
     5281 atatatcccc ttaccgtctg caccagctga ttggtcagtt tgctgcgctg aagcaaccac
     5341 agtcgggagc cgtaacggtc attctgtacc gcaggccagg tatcccccca ccatcccatc
     5401 gggacgtcgg cattgtcatc aggctccgcc cgccgccagg tgaacaggga aatcaccacg
     5461 gcgcgggtca gcggatccag cggtgcgctg gcgcaggtgc gtttaccgtt caccgtcagc
     5521 cacagttcca tcatgcctcc atcgctttat caggtttgtc ggtgttactg ccctgaccgt
     5581 tctctctgtg acgatgcccg ttataggcaa gccgcatcgc tgacatggtg gtgccgccgg
     5641 agtcgcacag gtctttcacc tgtccggtca cttccagatc catttcaaaa cgtgcttcag
     5701 gtgcattgcg aaacgtgatc gttttccctg caccgtccac cacgatcccc tcccgggtca
     5761 gcgtcacaga ctgcccctga tcgtcataga cagccacctc acccgtttgc agccctttca
     5821 ggcggtagcg ccggtccgac accgtaacaa ccaccgcatg agaacggtcg ccatccggaa
     5881 acaacaccac cgcttccgca ccgctgtttg cccttgcggt aaaaccgtag ggttcaagat
     5941 gttcaacccc ggctttgggt tcaccggcaa tcagggacac atccacggtc tgacatttcg
     6001 tggcggcact gatgcttttc accactgccc gcccaatcag gccgagaagt tgtcgctgca
     6061 tggcttcaat cgtcctcatc agaacgggtc ctcctgtact ctggcttttt tctttttccg
     6121 cgcgccgggg gcttcgggtt caggcagata agcgtcaggc gggccgacac ggatttccgt
     6181 cagggtgccg ttctggtcct gagtaaacgt gacttccgag acaagcagtt cggtattgtc
     6241 gaaaccacag accggatcga agacaatcac ccgctggttg ggctgccaca gcgtaccgtt
     6301 accctgtcgc cagccctgca ccacataggt ggtttcatcc gtccgcgccg cccgttttcg
     6361 ggcttcaaag tcagcacgcg caatacagcc tgcccccgtg gcctgccctg tctgcctgat
     6421 atacatcgga cggtaacggg caataaatgc gtcctctgtg cgggcccgca gcgcggtggt
     6481 ggtggcctca ccgaaatcat cgtcgtttcc ggcacgctgc cccgccacct ggtaaacaga
     6541 aaaccgctcc cggatactct tctccgtatc acaggaaagg atgttttccc caagtaccag
     6601 cgcggtatgt gcccgcgttg agccaatacc gccaatcacc agcctgccgt gcgggtcgtc
     6661 gtaagccagc gcctgctgct gaccgagtat tttgttgatt acctcaatca ccgtttcacc
     6721 gtgatcaggc tgaacatcag gaataacacc cgacggcgca ccgctgttca ccacctcaat
     6781 gccgaaaggc gcagcaagcg cctgcgcaat ctgcaccagc gagcgtccgt taaactgtgt
     6841 cggttcggct gcacagtcaa tcaggtcagc cgtcagacta cgtccggcaa taccggtgct
     6901 gaccgaacgg gcatcgtaac gaacgggagt cgcctccacc cagccggtga tcaccagctc
     6961 atcaccaatc agcacttcca cttttgaacc gtttttaatg cgcggctgaa gcgtggtgat
     7021 accctcatct cccggccatt ggcgagtgat ctccacactg aaatcccgcg ccagccgttc
     7081 aataccggca ccgatgcgca ccgatgtcca gccattccac tcccggccat ttacccgtag
     7141 cgtgacattg tcgttcattg cactggcacc ttcagaggga tcaccggcac aaagccggga
     7201 tgcgtaatgg cattacgccg aataatgtcc gcgtcacgcg ccgcgttatc aaaccaggtc
     7261 gccgccagca ccagcgcggg taaaacctca tccggcgtgc gctgaatgat ccgtgcagac
     7321 tgttcaaggc gcgtgttgat atccgcattc agatctgctt tcacccggcg cagcgccaga
     7381 aacagcgcat cactggttgt acgggacaac tccttatcaa ttgccgtatt cagtgtgtcg
     7441 cgaatgtcgg tcagttcttc ccacgttggc aggtcaaccg tgtttttcac cgccggtgca
     7501 ttgttcagtg ccggatgcgt gacagaaggc cagccggtgc tctgcgcggg tgttgttgac
     7561 tgccctactg tggcattctg catcaccgcg gaagttgtgg gcgcaggcaa tcgtgtgacg
     7621 gcatacgccg cttcgctgat tgcggtcgta cgaagggtgc tggcaaccac gttacgctgc
     7681 tgcgtcgccg tggcggtagt tttactgtcc gttttccaga cgccgcgcgg ttgcagatcg
     7741 ctgccgaggc tgacaccgga aagcgttttg atcatggtga ccaggtcgct ggcgttacca
     7801 taaaggcgtt tcccggtacg ccacattttc tgcacctgct caacgaaatt tttgcctgac
     7861 gatggtggcg gcagaagtac cgagatatcc ccctgcaaca gcctggcggc atccgatacg
     7921 gcagaatcca ccactttcat cgcatcagaa acatacccaa gcattgtgct ggcattaccg
     7981 acgacgtcgt tctgcacaaa atctgccacg ccatcgatac tgaaaccact gaagctgtca
     8041 ctgatgcagt catccagtgc agaacaggat gacatcagcg tctgcgccgt cgccgcacct
     8101 gaagtggggt aagagagttc tcccgcttcg acaaacttca ggtcaaagcg gacaatacgc
     8161 ccttcactct tcgatgtgct gacccgaact tccccgtcaa cacagacttt cagctcaccg
     8221 taagtcggat ggacaagcgt gccgggaccg ggtttattca gcgcgtcaat caggcgatcg
     8281 cgctggtcaa agcagtcatc tcccaccaca taagctgtga tggacgggcg gaaagtgatt
     8341 ttccccaggt cttcggtata gggtttgtcg cggttcgggt attcgtgcgt ttccacacga
     8401 cgaccggttc ccgcactttc ttcttcaacc ttaaacggca caccgcgaaa tgacgcgtcc
     8461 tgaagtctgt ctttccacgt catataaact ccgtacataa aaaatcccac cggagtggga
     8521 ctcattaaca gattaatttt tcattacctg ccaaagcgcg tatagccaac atcatggctg
     8581 acatcaaaac cgctggatcg cgtttccata acccgcatac ccggaggcga attcacaaaa
     8641 gagaccttga tctcaccatc aacttttggc gcagaagctt tgttaatcat gaagggattc
     8701 gggcctgtgg caccggaggc gttgtttgac tgagccggat ctaccgcctg ataaggtgtg
     8761 tatccccgtg ccggtattcc cgtcccataa gcatcataag cacccgcgcc ccactgcgca
     8821 gagttaatgg catcgaccgt gtcaccggaa ctgtcggtaa accactcaat aattggcttc
     8881 agtttgtccc acatatcctg aaaccactta acaaccggtc cccagttatt gattaccatc
     8941 cccagcggcg accaggcaaa aaccttcttc agaagttccc agccagcctc aaaataagga
     9001 ccaatggttt cccagagctt cttgaaataa ggtccgacaa catcccagtt agtgataatt
     9061 aatcccgcag ccagggctat cgccgtcgca atcattccaa tcggcgtcat cgacatgatc
     9121 ctgctgacaa tactgatggc accgccaacg cccatcaatc ccagtttcag aatcgcaaga
     9181 ccggcagcaa gcccgacgac gccgcgaata acccggggat tttcatccgc aaacttcgtg
     9241 aatttttccc ctaactcccc cagccattgc gtgatatttt tggcgtcacc agaaaatgcg
     9301 ccgccaatag ccgcaaggcc gttagttgcg gtccccgtca ttgcctccca caggttggac
     9361 agcgtaccaa gctgggcctg aacacgttta ttcaggctgg cctgtttatt catcttctgc
     9421 tggatctgat cgtagccatc ctttccttta tcgattagtg cattgaccac ctgaagggtt
     9481 tcggcatcat caccaaatat tgccttaagt acacctgttc gcttaacgtc ggtcagtttt
     9541 cgcagctttg ccagttgcct gaacatgtta tcaagaccgc caaaacttcc tttgccgtca
     9601 gtaaaatcga gctgtacccc gagtttctgg cgggccatga ttttattgac gtccctgatt
     9661 ttcttaacgc ttaatccgga ctggataact tttcgcaggg cgttacctgc cgactccccg
     9721 ttcatcccca tctgatccat catgacgctg atgggggcaa ggctctgtgc agcctgaaga
     9781 ccgtccttgt tcaccatctt cagaacagag ctggttttag tgaagaagga caacatgttg
     9841 gtatcgtcaa cacccagata aaacgccttc tggatagtgt cgaacagccc catcatgtct
     9901 tctgacgccg ttccggtagc atcctgcatc tttgcagcaa actcagcagc cgcttccggt
     9961 gtttttttca gttgtaccgc aagataagct gtcgctttac ccacaccgcc aagaatgttt
    10021 tctgccggga tcccctgacg caccagcatc tgcatcatgt tctggaaatc agccgttgta
    10081 ccgggtagct ggttacccag gccaatagcc agtttattga tgtcctgaaa gctctttccg
    10141 acctcgccgt tcgcatccat catggcgact ttcagcccgg tggcggcgtt ttcctgatcg
    10201 gcataagatt tcagggaaag cgtcagaccc gctgccagtc cgccaccaag cgccagccca
    10261 ccctgtgacg cttcttccgc ctggcgttta aatccccgga ttttcttttg cattttcgac
    10321 agcgcgggag aaagcctgtc gacaccggtg atcaacgcct taagctcaaa ttccgccatg
    10381 tgtgcgtttc tcctgctcta tcctgtttgc ctgactgacc agcaaggtaa tttcactgat
    10441 cggcatattc agcaattcga aaggattaat gcgccagtaa ctggcgcagt caaagaagcg
    10501 atcagtgagg tattcagccg tcaggcctgg aggaaaaaac cagccacaag ccacgccgct
    10561 gcattcaggt ctgccggaga catctggtcg acagagcttt gcggcacttt cgccagccgc
    10621 acaatgtatt tcgacaccac atgcgccaga agtctgactg actcatcctg attcatctgg
    10681 tagggatacc ccagctcgcg gacatccttc ccggtgggtt catcaaactc cagtacggag
    10741 agtgtctcgc catgagcagt aatcggtttc tttaactcaa gctctttcat tactggtaat
    10801 ccccttcttc accgtggaac tcaagatcaa ccgtgccttc ttcggcatta tggttcgctt
    10861 cgccgtgcag ccaggcagac gacagtacat agacctgacc gttcgccagc tcggcagtga
    10921 tggtcatctc atcagacgag gtgattttgc ttaccggaaa attcttcggc accttgaagg
    10981 tccctttgac ataaggcgca cggtgagttt ccttgcggtc cactgaaccg tccaggccga
    11041 tgatgtcatc gttaaccgtt ttgttcatgg gcacctcaat gccgccggtc agcgatagct
    11101 gctgaccgtc aattttgaaa taacaggttc ccccgatacg ggccattatg cagactcctc
    11161 tgaatactga agacggaact gattaaccac ggcaaagaca cgcagctggt taacatagtc
    11221 aggcgggaac agcgtgttca ggcggttcgg atcgctggca tcacgctcca caaccaggta
    11281 ctgcttaaac agttcgtagt tttccacgat ccccgcacgc tcaagctgac ggtaggttgc
    11341 cagcagttcc cctttgatca ccgccggggt gacaatcgcc tgaccgggac caaagcgggt
    11401 accgtcactg gcaagcttgt gacgcccgta cttactggta atgacggatt tcagtttgcg
    11461 cagtacatac gcgctggtat gcagcgtctc actgtcgagg taactgttat ccgcaacccc
    11521 gtaagcgttt ttcctgtacg tggtgacatc acgctgaatg cgtagtaccc cgctttcgac
    11581 atacgccgtt gccacgccat gagacagcag ggtctgttgt tcggtcatcg tgaaccgttt
    11641 ccccttcggc gcaggcagca tacccaccag ctcaccggtc tgcgtgggac gtgccggatc
    11701 gttgcggata aacaccgctg cgcgggcggt acggcttgcc gccagctcgt cggcaggcgt
    11761 ctgggtctct ttttcgtacc ccgccagggt aatgtgctgc tggttaaact ggtcacctgc
    11821 ggtcaccagt tctgacagcg tgccgatctt tgccgtatac acatgaccat acagctgacg
    11881 cgcatagctc cagcgaccgc tggtatcgtt catctcggtc accagcgtgt taacggaggc
    11941 cgtgtcgttg aacggcaggc cgatataatc aaacggctca tccgccattg cagccaccgc
    12001 gccggtgaga accggagcac ccgttccggc ggtacccgtc gccacggcaa tctgtacgcc
    12061 cgctggcagc acttcgcccc caccaaagcc gtagtaattg aggctgacag gaatttcatt
    12121 cccacaaagc cccttatgac gcgcggtcag tgtgaccacg cctgccgaag atgaagccgt
    12181 aaacggcagg gtcggaacgg cattgatggc atcctagata ctgctggcaa tcatcgtgac
    12241 gttatcgccg ttagtcaccg gtgcctgcac gcgggtacgt cctacataca cattcaccgt
    12301 gccggtttcg gttgccgccc cggtcaccgt cagcgtaact gttgccgccg cacctgtgga
    12361 ttcaggaacg gcaatcacat acagctcgcc aaacgggtca gtctggcgat aagcctcgac
    12421 catacgcgcc agctgacttc ccgcaccaca aatctggcgt gcatagtctg ccgacgacat
    12481 cagtaccaga ctgttggcaa caatctctgc accgttattg gcatgaccaa tcagcagcga
    12541 tgctccgctg tcctgtgcag tattcgccgc ctggttatcc atttccgcat aaaacaacgg
    12601 aaccagcgta ttcgacggaa tggtgttaaa gcttatcgtc atcggtgttc acctttttat
    12661 tcacgcgccg gatatcaccc gctgcttcac ggcgcagcca gtagttgttc tcgtcaacat
    12721 ttcgcccttc ggcgggcaaa aggtcgccgc gggcagggtc aggcactgac cgccctttaa
    12781 caggtttgac aaacatgagg atcctcagga aggaagggtt atttaggtgt gatgttcgat
    12841 atcgccgtca ggcccgttac cgggctcgag ataatcaaca tcaatcgcca gcgtttgcag
    12901 ttcatccaga ctgttcagat catcctgctg gcgggtatcg tcttcagtca gctcgctgat
    12961 gaccgaaaaa tcgaactgat aaatcagctc atgacgattc agatccagca gcgtgccgcc
    13021 gtcataggta atcgggttac cgcacgcctc cgggttccag cccagcagag ccttaaagag
    13081 catctgccgg acatcgtcca ccacatcata cgaggcaaac tgaccacgct catcacgccc
    13141 attactcagt atgacaacca cggagaaacc ctctttcagc tcctgccagt agtcggtctg
    13201 gcttttgttt tctcccggag aatcatcacc cggtacaaca tatgccgccg ggagtttcag
    13261 ctttccgacc tccggcagat ttttgaactg ggccgcgcct gcaacccggt tttcaaaata
    13321 cggacaacgg gcacgcagtg cagcaataac aggagtcagt ttcatctgtg tcgtcgctcc
    13381 ggcttcagtg atttacgcaa ttcccgcgcc agaaaatagc gtgtccagct gcggttcttt
    13441 tcaagcgttt ccaccatgaa gttattacgt ggagccagtc gccagccgct gccaccggat
    13501 gcaccacgat gatgactacg acgacgtttt gctcctcccc ggacaccaaa aaacagaaac
    13561 gccggataga agtcaccaga gatcatccgg ttccccttcc cgttgcgctg gttaggggca
    13621 atgcgtgtca taaaaccggc tcgcttttta ctggctctcg gcaccatata accaatcgaa
    13681 cgagccaggc gtccggtctg ataaccgggg ttttcacccg gtgccgaccg cgcacggcgc
    13741 atcaccagcc gacgggcatc acgcatatga cgctgcccaa tcgtgacaaa cgcccgccgg
    13801 acacgagcgc ggttaaagcg catctcggcg ggctgctgaa catcaacgtg aaaaaaggga
    13861 gtcgccattg ctgcctccgt gactctgcct acattcgccc agctccgtac actccagcag
    13921 cagaaagcgc cgcgccccgt tcagatcacg ctgacgtttc acccggtaca cactgtcatc
    13981 acagaccacc tcataatcag cagtgatccc ccggcggtaa cgaatggtga tgtaatgggt
    14041 gatggcgtcc ccggtctgcg cggtttcctg ccaggtggtg gcactggtct ggataacctt
    14101 cgcccatgtc cggaacgtaa ccgggtattg aggctccacg ccaaagttat ccgcgggcat
    14161 atccacccgc tggcggatca ggacgcgttt attcagttca ccggggtccg gcagaatgta
    14221 ggttgcgctg gtctgcgcct gacgaatttt cattgcggaa agtacctgta cgggccgaca
    14281 agccagccaa aactctgcgg catgtcgagt ttctccactt ccgtaaccga cgagcggttt
    14341 tcgtaaaaat ggctgataag catcagcatc cccagacgaa tatcatccgg caggtgcagc
    14401 ccgtccggat cgctgtccgg aatggtttca tccggtgcat agagcttccg gttcagatac
    14461 gtttccgtcc gcttttgtgc cgcacaggcc agcagttgca gatggcggtc atcagcatcg
    14521 aaatcctcat ccagccggag ttgggcttta atctcttcca ttgtcagaag catactcagc
    14581 cctctttact ggtcgtggct tttttctctt ttgtcgcttt actgcttttt gcactggttc
    14641 cgcgctctgc taacccggcc tgaagtgcaa tctcctgcac ccgggcagga agcgccccgt
    14701 cgtcatactc accggcccga atgacctcaa cacgcatacc gtccggtgac catttcagat
    14761 cttgtttcag gatcatgatt cttcacccgt cagaacaggg gcgcggttcc gcgcccctga
    14821 gtgattacgc cactgcaatc ttcagcagtt tgatggcctg cgaatcgacc agcatgccgc
    14881 cggtgcgttt ggtggtataa aaaccgacaa acggtttatt ggtgtacggg tcacgcagaa
    14941 tgcgggtgcc gatacggtca acgatggtgt aaccccgttt gaagttacca aatgcaatgg
    15001 ctttcgcatc agcggcgata tccggcatct gttcgttttc agcgataccg taacccgcca
    15061 gagaggacgg ctgccccagc tccagccccg gacgccacag atagttaccc tcgctgtctt
    15121 tcagcagacg gatggcaaac aggctattgt tgttcatcat gaacttcgcg ccggtgcggt
    15181 gtgccttacg cagcgtgtaa atcagtttga taatggcgtc tgcggtcacc gccgtcgcgt
    15241 cgccggatac aatatgctga agtttgccga acgcccggac cttatcggtt tcatccgtgg
    15301 attcattcgc caggaaccct ttcggcttct tggtaccatc gccggtggta aaggcaattt
    15361 cttcctgttc ggcaaattcg gttgccagct cgctgttgat ccatgcttcc acgttgaaaa
    15421 aggcatcatc cagcattttc tgggt
//
LOCUS       DAJTOR010000091        15289 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTOR010000091 DAJTOR010000000
VERSION     DAJTOR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.091"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(21..464)
                     /locus_tag="OO249_002512"
     CDS             complement(21..464)
                     /locus_tag="OO249_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR5636770.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGCIKRQMFTKLRTTKYLKTAASADSASVQFEGRVQRIA
                     RIHHYGLSDRVSRKGIYTIYAKRQLLGFNVQSIELIERMFIKNLT"
     gene            complement(457..888)
                     /locus_tag="OO249_002513"
     CDS             complement(457..888)
                     /locus_tag="OO249_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5636771.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVASGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSNVEAVAEPDEPEEMWTVKRG"
     gene            complement(984..1412)
                     /locus_tag="OO249_002514"
     CDS             complement(984..1412)
                     /locus_tag="OO249_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HCR5636772.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSVLLRQRQRRIEELKRENEDLRRWADT
                     PLPADIIRLRERPALTGGAAYRQWLSASDAVSAGAGSAAH"
     gene            complement(1409..1786)
                     /locus_tag="OO249_002515"
     CDS             complement(1409..1786)
                     /locus_tag="OO249_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636773.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALASLIKNGPLWQELRASLLKTITRKENVFISWVIRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITHNQMAQQVAA"
     gene            complement(1788..2300)
                     /locus_tag="OO249_002516"
     CDS             complement(1788..2300)
                     /locus_tag="OO249_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5636774.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALEKCVKQQPPQK
                     VYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(2281..2496)
                     /locus_tag="OO249_002517"
     CDS             complement(2281..2496)
                     /locus_tag="OO249_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636775.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(2500..2703)
                     /locus_tag="OO249_002518"
     CDS             complement(2500..2703)
                     /locus_tag="OO249_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCR5636776.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(2703..3167)
                     /locus_tag="OO249_002519"
     CDS             complement(2703..3167)
                     /locus_tag="OO249_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCR5636777.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERFQDYDATASGAKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(3263..3913)
                     /locus_tag="OO249_002520"
     CDS             complement(3263..3913)
                     /locus_tag="OO249_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HCR5636778.1"
                     /translation="MSLSPARQHRLRVQAEQAVREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLTEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(3917..4975)
                     /locus_tag="OO249_002521"
     CDS             complement(3917..4975)
                     /locus_tag="OO249_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HCR5636779.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQISDFSAAAAETGA"
     gene            complement(4992..5825)
                     /locus_tag="OO249_002522"
     CDS             complement(4992..5825)
                     /locus_tag="OO249_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCR5636780.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTFMEVSLKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEVQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            5968..7734
                     /locus_tag="OO249_002523"
     CDS             5968..7734
                     /locus_tag="OO249_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HCR5636781.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDDQIEKLEKIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGRLCADGQYRQIVT
                     VEDAVRGACNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            7734..8330
                     /locus_tag="OO249_002524"
     CDS             7734..8330
                     /locus_tag="OO249_002524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636782.1"
                     /translation="MGKSKKNRAAATKQTQHKNQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILISTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRR"
     gene            8364..8765
                     /locus_tag="OO249_002525"
     CDS             8364..8765
                     /locus_tag="OO249_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5636783.1"
                     /translation="MYMTDAAQNQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGL
                     QIIPLSEVAAKDEFLNIKNVSRDDMMAAHRVPPQMMGIMPNNVGGFGDVEKASLEFVR
                     NELIPLQKRMMEINKLVSENIVKFDSYKLAT"
     gene            complement(8872..9753)
                     /locus_tag="OO249_002526"
     CDS             complement(8872..9753)
                     /locus_tag="OO249_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636784.1"
                     /translation="MKWAIEMNKLKRPVFKDYSAFKKLSANKRLKHHSVLNGQDTFIL
                     NAYRSYIKNKGYLNVNSCGVLSNNIAEALRYYYNNPAKCLSIIDDIRAANSNSLCPMC
                     GSMHSGTLDHVLPKENYPEFSLFTKNLVPACKCNTFKSTTIANSAGHRMLHPYFDNIL
                     KQRLICANFSKLGRTPTIDVKIIIDPMSPEYNNVKYHLDNVVIKNNIKGYLSEQWESF
                     YLYPELVIRGLNKSLSTYSDVYELLTRELLLLDEKHKGKNNWNSVFVAGLIRPDVIRW
                     ILCELHLGNRSPDGRLI"
     gene            complement(9737..11386)
                     /locus_tag="OO249_002527"
     CDS             complement(9737..11386)
                     /locus_tag="OO249_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5636785.1"
                     /translation="MLVKKNKNYSLYFLITNNQQISDSGNYIKIVPSRSDWNDFGYLS
                     RVEISIFNKKECKDIISLNGYIGFLNSEEERNGKAELIRRVKKKEESNLKDEGFNDYF
                     VMLQDMNNYRRLVRFFGVNESKYILNFINDVVSKKSDASAENLRKKAISSDIFNKSFI
                     RESESYFTFKNAGTVLSGIQYEELGNLSQHIKISYSNKVNEEDVSYVFNFDHEHDLPK
                     RISIIIGENGVGKSQTLREIALAAIKGKDNLVAVVDSNGVSIEERIQISRLIAFSPTN
                     ESKCSFPTDKRIKSLIWYKRLTLNRESKSKLNLNDLIVQLARSNDAIGYNQRWNIFVD
                     ALSVLNSNNDLVIKCGGENSPFVKLNELNSRGEESRLSLYSEIILSGEPLRLIENKCY
                     PLSSGEISFIKFCVQVCLYIENGTLLLIDEPETHLHPSFINKFMALLDNLLEKTGSSA
                     IIATHSVYLVREVFKEQVTILRRRDDGVIVSEKPRLSTFGANIGNISYFVFGESEPTQ
                     ILNKVKNNIIAKNMSWDDVESKYSEDLSLNVLTALRNEMGN"
     gene            complement(11679..11912)
                     /locus_tag="OO249_002528"
     CDS             complement(11679..11912)
                     /locus_tag="OO249_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HCR5636786.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(11923..12111)
                     /locus_tag="OO249_002529"
     CDS             complement(11923..12111)
                     /locus_tag="OO249_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636787.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            complement(12265..14679)
                     /locus_tag="OO249_002530"
     CDS             complement(12265..14679)
                     /locus_tag="OO249_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCR5636788.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREALSDQVWERYFFYESRDPVQHEMEQDKLISRAKLAHEQQR
                     FNPDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHVGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEEG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNPWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMRQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCL
                     LAENLNQQEKDKSADGNPRTDITSMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     QWRNLY"
     gene            complement(14676..>15289)
                     /locus_tag="OO249_002531"
     CDS             complement(14676..>15289)
                     /locus_tag="OO249_002531"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HCR5636789.1"
                     /translation="REVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRGLCRYNKS
                     GHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVVYCDPPYDG
                     TFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKVKR
                     SIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVDSSAVYGVRA"
BASE COUNT         3736 a         4134 c         3517 g         3902 t
ORIGIN      
        1 caattgaaaa caaataaaaa tcaagttagg tttttaatga acattctttc aattaactca
       61 atagattgga cattaaaacc taataactgc cttttcgcat agatggtata aatacctttt
      121 cgactgacgc gatcactcaa gccgtaatgg tgaatacggg caatgcgctg caccctacct
      181 tcaaactgta cgctggcaga atcggcgctg gctgcagttt tcaggtattt tgtggtgcgc
      241 agctttgtaa acatctgacg tttgatgcac ccctttttgc tgcgtgctgt taccctgcgc
      301 ggctcataac tgctgccatc aggattgcgc tgcatcctga tgttctgctg ctgtgtccgg
      361 cgcagttcct gcgccagctg gcgcatcatg cggcttcttg cggctggctc cagattcgcc
      421 aataatgcac tcagccagtc gtccaccttc tgcaattcag ccacgtttca ccgtccacat
      481 ttcttcaggt tcatcaggtt ccgctaccgc ttcaacgttc gacacactgc cgtcagtgct
      541 gaccagcaca cgctccgtca gttgcaggtt aaggctgata tcacagacat cgttgcgcag
      601 aatatccacc tcaaaggtga atagcttttc ccgtaacgcc gggttattga tcgcatcagg
      661 ctggttatcc cgcagccaca gcaaaaccgg ggccatcagc agattctggt cgccgctgaa
      721 atcttcaatc accacgttca gggtgtagcg gtactcccat gacatggagc tagccccgga
      781 ggcaaccagc gaaccgttat ccacaaacag atgcagtttg tccgggttat tacggacata
      841 aggcactgct ttattgaggg cgtggcgcag ggattgtggt ttgttcactg tttcgctcct
      901 gacacgcaat aatcatgtcc actttgtctg cacagaccgc ccaggcggcc tccgtttcat
      961 ccagcaacgc attcaaatca ccgttagtgc gcggcgctgc ctgctccagc cgacacggcg
     1021 tcactcgcgg acaaccactg acggtaagct gcacctccgg tgagtgccgg acgttcccgc
     1081 agccggataa tgtcagcagg caaaggagta tcagcccagc gacgtaaatc ctcgttctca
     1141 cgtttcagtt cctcaatccg gcgttgtcgt tgtctcagca gtacactggt ctgttctgct
     1201 tcggcataga gccgcgcctg ctcccggtta ttggtttcag tcagaatgga caggctgata
     1261 agctggctgt tgctctttgc cagtgcctgg cttttgctct gcagctcgtc tgcctgcgtg
     1321 ctgatggtct ggctggcatc agccagccgc cacgtctgcc agcccagcgc cgccaataat
     1381 aacgccagca caaccagcag caaccggttc atgcggctac ctgttgcgcc atctgattat
     1441 gggtgatcca gaaggcaata acggtcagca gataaaagac cagggtaatg gcccaccccg
     1501 tccaggcgag actgacgaca atcagcaatc gtatcaccca gctgataaat acgttttctt
     1561 ttcgggtaat tgtcttcagt aaagatgccc tcaactcctg ccagagcggg ccattcttaa
     1621 ttaacgaagc cagtgctacc ggaattaccg cccatgtcag taaacaggct acccaaacgc
     1681 cggacgctgc cagtaccgga aaaatcccct gcggatacac cattgctgcg attaacagcg
     1741 ccatccataa catcagaaac agcccgctga ttaatttctt tttcatttca gtttgctccc
     1801 tgtaaacacc aggccatctc ccgcgcacgg cggttatcca gcccctgatt aaaaacacct
     1861 ttcacataaa cccagcgcgg caactgtcgg cacgcatccg cccagcgccg ctgattgagc
     1921 aatttcacca gcgtggaact gcaggcattg cccgtaccca cgttgaaggc aaacgacacc
     1981 gcagcgtcat acaccttctg cggcggctgt tgcttcacac acttttccag cgcccgctcc
     2041 acacgcagca cgttggagat cagcccttct gctgcctgtc gctccgtaat ggttttgccg
     2101 ggaatgacgc ccgacgtatt accaataccg tcggtccaga cacccgcgct gcactgatac
     2161 ggctgcagac gacaaccttc gtaatcggca atcagtttca gtccctccac ggaggtgtga
     2221 agctgctgaa aacccggcag cgtggcagca atagccagca cggccccgac aaggcagcgt
     2281 ttaacgattg atggattcat agtcctcccg cgagatctgc ccgtcgcgca gaagctggta
     2341 ggctttgtgt ttgtagtacc agttgatagc cagcatcagc acaccaatca tcaggccgcc
     2401 cagcgttgag gcatccttga tggacaaatc gcccagccag gccagcacga cggcgatgca
     2461 atacgtgata aaggcgctga ttcgctcaag cgtcataatt cagtcccata gctggacggt
     2521 ctgcacggtg gtggtggtcg gaatgtccgg cagctccacc tgcagcccgt gaggtaaaaa
     2581 ggggccgtat tcggcaagcc ccggatttgc cttcagtacc tgctccgtga caccctgcgt
     2641 gcgcccgtaa tgacgccagc aaagcgcgtc caccgtgtca tactgatgcg cacgcacttt
     2701 catcagataa gctccactgt gcagtgcggc gcgtcctgca cccggctgat ggcccagcgg
     2761 gcgtcacgcc acaaatcacc gcttgcttcc gccagttctt cgccccgctt cgcaccggat
     2821 gccgtggcgt catagtcctg gaaacgttcg ttgagcatgg cgcgagccca gcagtaaacc
     2881 gcgttgaaat agtgctgaat gcgctcactt ttcccgtcca gctgttccgc cggaacctca
     2941 gccagcgacg cataccccag catctgctgg cgtctgcgaa actcatacag ctctgcgtta
     3001 acctccgaaa ttgccgacag tgcaacctgc tttaaacgcg gctgcgtcac cgtgccgtca
     3061 gtgcgcatga cactgcgaaa ctccgacagg tccacatcag gccagaacgg cgtatttcta
     3121 atgatttccg cctgttccgg tgcctgttct ggcgcaacaa acttcatgct gctttctcct
     3181 gaaataaagg gcggtggacg gggttttgat gtggctgtgc ctttcgccac cccgtgccgc
     3241 ccgtgcgcgg gggcacgttc tgtcagcggc tgtcattgcg cagtctgcgc tccagctgct
     3301 gtttgtcttt tttcacgcca cagcggggat cgagctgtaa cgcatggttg agatgattaa
     3361 gggcggaagc cggattgctt tcactcagga ccgcgccaat cgctttatgc agacgtgccc
     3421 gtgactggtc cggcatatcc agaccgtctg tcagctccag cgtctgcagc aacagatcgg
     3481 catcaaagcc ggtggcggca agcattgcgc tctgcgctgc atctgccatt tcctctgtca
     3541 gcacggtctg cacgttgcgg ttgcccagcg gcatcaccca tccatgacgc agggcatgac
     3601 gcccgatctc cagcgccccg gcataatctc cggcatcaat gcgccacaac atcacgtaca
     3661 tcagcacgtc atcctgttga gcgcctccgg cagccaggac accctccgcc caggcggcgt
     3721 acttcggcag cagctccact ttgatttccg cttttttgac cgtggactga acgcccttga
     3781 gacggcggcg gtcttccgcc agttgcagca gcatcaggtc atagcccgat gcgtggcgaa
     3841 cactgccacc ctcacggacg gcctgttcag cctgaacgcg caggcgatgc tgccgtgcgg
     3901 gactcaggct catggattac gctccggttt cggctgcggc ggcgctgaaa tcactaatct
     3961 ggatgttttc caccagtgcg gcgcagcggt agtcctcaac cacataggct tcgttaacgg
     4021 attcaaagtt ttcaatccgg tcacgtttcg ggttgtcgat aactgaacgg cggcgggtgt
     4081 cttcctgcca gtagatggac aggttatcca gacgggtgat cagcagcgca ttcggcggga
     4141 agaacggcgc acgcacggcc tgcaggccac ccatgcgttt ctgactgatg atcatatcgg
     4201 cagccagttt ttcactgttt tcctgctctt tgttgaccag cgggaaatac ttgtcagaca
     4261 gcagttcacg accgcaaatc accaccagat cgtcatcgtc ctggtagacc acgtcgataa
     4321 gctcattgac ggcatccatc accacggcgt ccaggttggc atattcgcca cctttcccga
     4381 ctttcactgc gcccggtgtg gtttcaccgc ccgtggtggt gctgcccatg acgtgatccg
     4441 gtgcatcctc acggattttc tgcagccagc ctttattcac atcctgcagc agcgggtttt
     4501 cgctacggtt ggaggttttc gcacgcttca cgccattaaa gccgatcatg atgcggtcca
     4561 gtgcctgacg tttcacgatg gcgtcacgga tacgcacctg aaaatcctga aacttcgccc
     4621 acaggtccag cttcgcgtag gtcagcaccg tgtcaaagtt ggtctgctcg catttatatt
     4681 ccacatcgac catcagcgtc ggatcgacag gttcacgctc tttcgcagtg gtatcagtgg
     4741 ttccggcaat ggtgctgcca actcccaacc ccagcagctg accggactgc tcagtcactg
     4801 gcgtgacgtt aatcagagtc aggaaagcgg cggactgctg gatctggtct tccagcgtct
     4861 gctgcacgga cggctctacg gtgaacttgc tggacagttc ttcaactgcc acaccgttca
     4921 gacgcgccag ttgctgcagg taagcgttaa aagcaaagcg ggtattcttc ttcatcaggt
     4981 tttgtgctcc atcagcaatt ggtcagagtg tcagcggggg cgttaccgcc tgttgcacgc
     5041 tggcggtagt cctggcggct gtcttcatga ctcagcttgt ccaccagttc gttaaaggcg
     5101 gtttgctgtg cctgcagggc agtctccagc tcagacaggc gttcttcctg ctcagacagg
     5161 gatttttcgg tgcgtgcgct caggttctgc tgctcagtgg cgaccagctc cacggcctta
     5221 tgcacatcag agaaccgggc gtcatcggac tgctcttttt tggtaaacag cgccgtgacg
     5281 cgggcaaaca gggacggttt gtcgtcctgg acttcttcca gttcgatcac cgtttcctct
     5341 gcggcggtaa agagattggc gggattctgc ttgcggtttg ccagcgggtt atgggctgca
     5401 ctggcgctga atgtcagcat ttcagtgccc agactggcag ggtcatcagt ggcagccagg
     5461 ccgactaggt aggctttgcc cgtatcagcg aacttcaggc tgacttccat aaaggtgaat
     5521 aatttctggc cttttttcac cagttccacc agggactccg ttggctcaac gtcagcatac
     5581 agcgccatct tgcctgccag cggaccttcc gtgatttctt cagcaaacag cgccgtcacc
     5641 ttgccgtagc ggttaaaggt gctgtccggc agataagact tgatgtgctc aaggttaatc
     5701 agcgcggtat acaccgccgg gttatagctg gctgccatct gttccagcca ttcacgctgg
     5761 atttcgcgtc cgtcggtggt ggcaccttcc accccgatgc gaaaacgctt tgctttcact
     5821 gtcatgagcc gtgctccgtt agaaaaaact tactggagcc ttatggttgc ggtgataggg
     5881 gcagtgaaac aatgcgcggt atttgtaccg acaaccacac aaaccgcagg cggggaaagc
     5941 cttcattcaa ggctgtaggt ttgtgccatg aacaccacac tgacacccgc agatctcgat
     6001 ccccgtcggc aggccatgct gctgtacttt cagggatacc gcgtagcccg cattgctgaa
     6061 atgctgggcg agaaagttgc aaccgttcac agctggaaaa aacgcgacaa gtggggtgac
     6121 tatgggcctc tggatcagat gcagctcacc accgccgcac gctactgcca gctcatcatg
     6181 aaggagcaca aagaagggaa agatttcaaa gagattgacc tgctggcgcg ccagtctgag
     6241 cgccacgcgc ggatcggcaa gtttaacaat ggcggcaacg aagccgactt aaaccctaac
     6301 gtcgccaacc gcaacaaagg cccacgccgt cagcctgaaa agaatgtttt caccgatgac
     6361 cagattgaga agctggaaaa aattttccat tcctccatgt tcaactacca gcgccactgg
     6421 tgggaagccg gaaaaaccaa ccgcatccgc aacctgctga agtcacgcca gatcggcgcg
     6481 accttctatt ttgcccgtga agccctgatt gacgccctgc ttaccggacg taaccagatt
     6541 ttcctttctg ccagtaaggc acaggcccac gtcttcaaac agtacatcat cgactttgcc
     6601 aaagaagtgg aggtggagct gaaaggcgat ccgatggtgc ttcccaacgg ggccacactg
     6661 tatttcctcg gcaccaatgc ccgcactgcc cagagttatc acggtaacct gtatctggat
     6721 gaatatttct ggataccgaa atttcaggag ctacgcaaag tggcttccgg tatggctatt
     6781 cacaagaaat ggcggcagac ctatttttcc acaccatcca gtctgaccca cagtgcttat
     6841 ccgttctggt ccggtgcgct gttcaaccgt gggcgcaaca aagctgacaa ggtggacatc
     6901 gacctgtccc acagcaatct ggcccccggc cggctgtgcg cagacgggca ataccgccag
     6961 atagtcaccg tggaagatgc ggtgcgcggc gcctgtaacc tgttcgacct tgaccagttg
     7021 cgcatggagt acagccctga cgaataccag aacctgctga tgtgcgagtt cgtggacgat
     7081 ctcgcgtccg tgttcccgct cagcgaattg caggcgtgca tggtggacag ctgggaagtc
     7141 tggaccgact ttcatgcact ggccctgcgc ccgtttggct ggcgcgaagt gtggatcggt
     7201 tatgacccgg caaaaggtac gcagaacggc gacagcgccg gatgcgtggt ggtggcaccg
     7261 ccagccgtgc caggcggtaa gttccgcatt cttgagcgtc accagtggcg cggaatggac
     7321 tttcgcgccc aggctgacgc catcaaaaaa ctgactgaac agtacaacgt gacctatatc
     7381 ggcatcgact caaccggcgt tggtcacggg gtttacgaga acgtgaaagc gttttttcct
     7441 gccgtccggg agtttgtcta caaccccaac gttaaaaacg ccctggtact caaggcctac
     7501 gacattatca gccaccgccg tcttgagttt gacgccgggc acaccgacat tgcgcagtca
     7561 ttcatggcaa tccgtcgcgc caccaccgcc agcggcaacc gcccgaccta tgaagccagc
     7621 cgcagcgaag aagccagcca tgccgatctg gcctgggcaa caatgcacgc actgtttaac
     7681 gaaccgctgc agggcgagtc cgccaatacc agcaatattg tggagatttt ttgatgggaa
     7741 agagtaagaa gaaccgcgct gcggcgacga aacagaccca gcataaaaac cagacttcag
     7801 ccgaagcatt cagcttcggc gatcccgttc cggttctgga ccgccgtgaa ctactggact
     7861 atgtggaatg cgtacagaca gatcgctggt atgagccgcc agtgagtttt gacggactgg
     7921 cgcgcacctt ccgcgccgcc gtgcatcaca gttcaccaat tgcggtgaaa tgcaacattc
     7981 tgatcagtac ctacatccct cacccgctgc tcagccagca ggctttttca cgttttgtgc
     8041 aggattatct ggtatttggt aacgcttacc tggagaaacg cacaaaccgg ttcggagagg
     8101 tcatcgctct tgagcctgct ctggcaaaat acacccgacg cggattagac ctagatacct
     8161 actggtttgt gcaatacggc atgaccacgc agccgtatca gttcacgaaa ggaagcatct
     8221 ttcatctgat ggaaccggac atcaaccaag agatctacgg cctgccaggt tacctttctg
     8281 ccattccgtc agccctgctc aacgagtccg ccacgctgtt ccgccgatag tattacatta
     8341 acggcagtca tgcaggcttc atcatgtaca tgaccgatgc cgcgcaaaac caggaggatg
     8401 tgaacaacct ccgcaatgcg atgaaaagcg ccaaaggacc aggtaacttc cgcaatctgt
     8461 ttatgtactc gcctaacggc aaaaaggacg ggcttcagat catcccgttg tcagaagtcg
     8521 cggcgaagga tgagtttctg aacatcaaga acgtgagccg ggatgacatg atggcggcgc
     8581 atcgtgtgcc accgcaaatg atggggatta tgcctaataa tgtcgggggg tttggggatg
     8641 tggagaaggc gagcctggag tttgtacgta acgagttaat accgttacaa aaaagaatga
     8701 tggaaataaa taaattggta agtgagaaca ttgttaaatt tgatagttat aaactagcta
     8761 cttaacgtaa tcactaaatg aggttcttta acacagctaa aaaatttatt aatagccagc
     8821 agtttttatg aatttcttca gcgtgcagat acacgctgaa gatgcaaaaa atcatatgag
     8881 acgaccgtct ggacttctgt tacctaaatg taattcacag agtatccacc taatcacatc
     8941 aggtcgaatt aacccagcta caaaaaccga attccaatta tttttacctt tatgtttttc
     9001 atccaacagc aacaactcgc gagtcaacaa ctcatataca tcgctataag tagaaagact
     9061 cttattcagt cctctaataa caagctcagg atataagtaa aacgactccc attgttcaga
     9121 caaatacccc ttaatattat tcttgataac aacattatca agatgatact taacattgtt
     9181 atattctggt gacattgggt caattatgat ttttacatca attgtaggtg ttcgccctaa
     9241 tttagaaaaa ttagcacata ttaatctttg ttttaatata ttatcaaaat atggatgcag
     9301 catccgatgg cctgcagaat tagctatagt agtacttttg aaagtattac atttgcaagc
     9361 aggaaccaaa ttcttagtga ataaagaaaa ttctggatag ttttccttgg gtaaaacatg
     9421 atccaaagtc ccgctatgca ttgaaccaca cattggacat agagagttag aattagctgc
     9481 tcttatatca tcaattatag ataaacactt tgcgggattg ttataataat atctcaaagc
     9541 ttcagcaata ttgttactca atacgccaca agaattgaca ttaagatacc ctttattctt
     9601 tatgtacgat ctatatgcat taagtataaa tgtatcctgt ccattcagaa cagaatgatg
     9661 ttttaatctc ttatttgcag atagtttttt aaatgcagaa taatctttaa aaacaggtct
     9721 ttttaattta ttcatttcaa ttgcccattt catttctcaa cgcagttaat acattaagtg
     9781 ataagtcctc gctatattta gattcaacat catcccaaga catattcttt gcaattatat
     9841 tgttttttac tttatttaaa atttgagttg gttcactctc accgaaaaca aaatatgaga
     9901 tatttcctat attcgcccca aaagtactca atctcggttt ttcgctaaca attaccccat
     9961 catctcgtct tctgagtatt gtcacttgct ccttaaaaac ctcccgaaca agataaaccg
    10021 aatgcgttgc tatgatagct gaagagcctg tcttttctaa taaattatca agcaatgcca
    10081 taaatttatt aataaatgaa ggatggagat gtgtttctgg ctcatctatt aatagaagag
    10141 tcccattctc aatatacaaa cagacttgta cacaaaactt aataaacgat atttccccac
    10201 tactcaaagg gtaacattta ttttcaataa gcctcaaagg ctcgccagag agaataattt
    10261 cagaatataa tgatagtcta gattcttcgc ctcgagaatt aagctcattt aatttaacaa
    10321 atggtgaatt ttcaccgcca catttgatta ctaaatcgtt gttactattt agaacactca
    10381 gtgcatcaac aaatatattc cacctttgat tatagccgat agcgtcgtta ctcctcgcta
    10441 gttgcactat taaatcattc aagtttaatt tggattttga ttcacgattt aaagtcaatc
    10501 ttttgtacca tatcaatgat tttattcttt tatcggttgg aaatgaacac ttactttcat
    10561 ttgttggaga aaaagcaatg agcctactaa tttgaattcg ttcttcaatg ctcacaccgt
    10621 tagaatcaac tacagccacc aaattatcct ttcctttaat cgcagccaat gcgatttctc
    10681 taagcgtttg gctttttcct acaccattct cgcctataat tatggaaatt ctttttggta
    10741 aatcatgctc gtgatcaaaa ttaaaaacat aagatacatc ttcttcatta actttattcg
    10801 aatagctaat cttgatgtgc tgtgataaat tacccaactc ctcatattga ataccggaaa
    10861 gaactgttcc tgcatttttg aaagtaaaat acgactcaga ctctctaatg aaagatttat
    10921 taaagatatc agaagagatt gcttttttcc tcaaattctc tgcactagcg tcacttttct
    10981 tagaaacaac atcattaatg aaatttaata tatatttgga ttcattcacc ccaaaaaatc
    11041 ttactagtct tcgataatta ttcatatcct gaagcatgac aaaataatca ttaaacccct
    11101 catcctttaa attagattct tcctttttct tcactcgcct tatcagttct gcttttccat
    11161 ttcgttcctc ctctgaattc aaaaaaccaa tataaccatt cagtgaaatt atatccttac
    11221 actctttttt attaaaaata gatatttcga ctctactcaa atatccaaaa tcattccaat
    11281 cgcttcttga tgggactatt tttatataat taccagaatc agatatctgt tgattatttg
    11341 taattaaaaa atataaacta taatttttat ttttcttgac taacattatt accctctcac
    11401 ttcattagtg atgatttatt ttggaattat tttacacact tatacctagc gcgcaatcgt
    11461 atccccgcca cgcctgcccg ctttatgtag aggttttcat gcacctgcat gatctacgca
    11521 aaagcccgcc agttctggcg ggccttagca aaaacgatcc tcaaacgatc atgcgatctc
    11581 atgcggcata gacatgcact acagagctaa cgcctcgcaa gggctcgttg ttcaaccttg
    11641 ctgacgccag aagctagttc agacgccagc aacgtttctt aatgcagcca gctgtcgtct
    11701 tcccacacct tctgcataat tttcatcact tgttttcttt cttcgtccag ttgcaatcca
    11761 gtaagttcca caccgttaga gctacctttg cggatacgaa ttaccgtttt gggatacaga
    11821 gggcgcagat tgcggtaaag ctcggattca agagcgtcca gggtagactg gctaatcttc
    11881 tgctctttat cgatcattat ttcaatgcgc ataaaagtca cctcagctga tgacatccat
    11941 tgagcggttg tattcgtggg ttctgatttt tgccatgagt tcatcagtca attcagaaac
    12001 ccactgcaga gccagcccct tctcttcatc actacactca ctagccgcta caagcttaag
    12061 aaaaaaatca atgcgctgga gcttcaaaga ctccaaaaaa tagtcctgca tctttcctcc
    12121 tatgacacca cacgcaatac tgtatgcata accactgttt atatttacag catataataa
    12181 tcttactgat gtaaaacgtt tttttacgtt catcagcctg atatgcctgg tattattaag
    12241 agcacgaatt gttaacccgc gtaattaata caggttccgc cactgatcat cttcctgcaa
    12301 acgctggttc cgatagaaga tacgcaggcc tgctcctgac ggaatactgc ctccgcgaag
    12361 gagtaaatcg acctctttct cgctgccatc aaatcctctg gacttcagct catacacgag
    12421 ctgcagtcgc tgatggtctg taattcgctg tttgtagtct ttacgccgtt tcggtttcac
    12481 caggcgtaac cttgctgcca gttcccggcg ctcttttttg ctcatactgt gcaggtaatc
    12541 gtgcaactcc ttgtcatcca tgctggtaat gtccgttctg gggttcccat cagctgattt
    12601 atctttctcc tgttggttca aattttcagc aaggagacag ttattgccac gagtccaagg
    12661 ggcgcaagcg ccctggtcgg ctgccgcctc ctgaacgtca acggctttac gaaccatttt
    12721 ccacttcact gcatgagtgc agatcttgcc ttctgcaatg ggtgaccaga tgccataaat
    12781 acgaataccg tgatcgccat aggcggtcgg ctcttcattg atttcataag cggttctgat
    12841 caagtgatat ttgcgaggaa ccagcacgcc gccctgcctc ataatgtagg tggcaaaaca
    12901 gccagcatca gcagcagcca ggatggcatc aaggcgcggg ttatccagta ccggcgcacc
    12961 tgcttttttg tcaccctgct gccttgccgc ctgaccagcc aacaaccgca gttcacggta
    13021 agcctgacgc cctggaatac caaagaagcg gaattgctga acacgatgca gagacgccca
    13081 gggattaacg tattcagcgt tatcacgcag ggatttaccc gtttccttgc tgatctcgcc
    13141 agccagacca cgcccgtcaa tgttcttact gatgtatttc gcgatgtagc ttgtcggcgt
    13201 tcctttgcgc gggtttatca gctcagattt aaagcgtgga ccagtgttat tacccagttc
    13261 ctcgcggtct tcacggatgg caaacttacg caacaatgca gtaatggcgc ggcggtcttt
    13321 tttgcgcata aaacacaaca ggtgccagtg aactgtaccg tcatgatgcg gctcagccac
    13381 ccgcacgcca taccagcgca acccggcttt gtgcatcgcc ttacgaaatg cagcaaacat
    13441 gccgaccaga taatcgctgc tttgtcttac cgtcgcattt gtccaggttg ggtttggtct
    13501 gccgttattg agcgtggaat ggaaacgtga cggacaggta atggtgtaga aaacggcgca
    13561 gtcaccgcgc atttccgcga taagctccag acctttaaca caggccatca tctcattgcg
    13621 gcggtgcgcc gggttgctgc tgctggcgtt taccacgtct tccatatcca gcgtgtcgcc
    13681 ctcttcgttc accagttcat gagaacggaa aaactccaat gacttgcggc gctgctcacg
    13741 tttatgcatc acggcttcat agctgacata gggagatgct tttttgctga ccaggcagac
    13801 agcacgcaac tgctcttccc gccattcgca acgcattttc cataatttcc ggtaccacca
    13861 gtcggcgcac aacatacgcg ccagcgaacc cggaatgagt tcatagggca cgggtttacg
    13921 gcggtttctt ttccgacgga gttgctcaaa cgcaggcggg atgacatcca gacgcagggt
    13981 ttccgctgcc accttttccc atgtcttgcg gatttcttct ggcttaacgt catcggtggc
    14041 atacaaatca ccacaagctg catcaaggca catgctcata tgcgcagcga caagggtaga
    14101 caggcgtttc acctgatcct gactcatttc aggcagaatc agcaggccgt ccagccctac
    14161 atggcttgcc ataaaacgaa aagatgcaga tagctggctg tcgcgtacat gctccagtcg
    14221 ttccagacat ggcttaatcg tctcacgtaa atagcgggaa taagcctttg gcctgcccag
    14281 gctgctgaag tattcaatac gttgcatcag cggcttgctg atatgggaag gctgggcgtt
    14341 gacgtccgcc agaatgacca tatccggatt aaaacgctgc tgctcatgcg ccagctttgc
    14401 ccggctaatg agcttatcct gctccatttc gtgctggaca ggatcacgtg attcataaaa
    14461 aaaataacgc tcccagacct gatcactcag tgcctcgcgg cgcaactgtt cctgctcgtt
    14521 atcggcagcg tacagagtga tcaggtttga aagcgcagaa accggcgcaa cttccgccgg
    14581 gtccagataa gggttaatgg cctttttcgg gctgttccat gagaacgctg cggcagcctc
    14641 gttaaagccg cagcagttgt tcatatcggc atggctcatg cacgtactcc gtacacggca
    14701 gaactatcca cgccacgcga ataatcaaat cccacccagc agcgcggccc ggaaacagca
    14761 atgatttctg ttgctgattt accctcgcca gctgccacac cgatgctgcg ttttaccttg
    14821 atatagtggt gagtaaaatt gcgatacagc gaacggatca gggatgtgtc actgttagaa
    14881 acaatgaccg gatgtccttc tgatgaccga tgttcaagaa cggatgctag gtgatactgg
    14941 tcatcttcag tgaaaccatc agtgtgatag ccggaaaacg taccgtcata cggtggatcg
    15001 caatacacca catcccccgc cttcaacatc gccagcgttt catcaaagct ggcgcagata
    15061 aacgttgctc gctgggcttt ttctgcaaat gcgcgaattt ctttttcagg gaaatacgga
    15121 tttttataat tgccgtaggg aatgttgaaa tgcccgctct tgttatagcg acataaacca
    15181 cggtaaccgt gacgattgag atacaggaaa tataccgctt tcatgaaatc agtaatttca
    15241 gttgagtaat taaactcctg ccttatgttg taataagcca cctccctgt
//
LOCUS       DAJTOR010000092        15261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTOR010000092 DAJTOR010000000
VERSION     DAJTOR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.092"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..270)
                     /locus_tag="OO249_002532"
     CDS             complement(<1..270)
                     /locus_tag="OO249_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5636790.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVA"
     gene            complement(275..460)
                     /locus_tag="OO249_002533"
     CDS             complement(275..460)
                     /locus_tag="OO249_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5636791.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            complement(457..3096)
                     /locus_tag="OO249_002534"
     CDS             complement(457..3096)
                     /locus_tag="OO249_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5636792.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3304..4293
                     /gene="ldhA"
                     /locus_tag="OO249_002535"
     CDS             3304..4293
                     /gene="ldhA"
                     /locus_tag="OO249_002535"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5636793.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4404..4826
                     /gene="hslJ"
                     /locus_tag="OO249_002536"
     CDS             4404..4826
                     /gene="hslJ"
                     /locus_tag="OO249_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5636794.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4823..5089)
                     /locus_tag="OO249_002537"
     CDS             complement(4823..5089)
                     /locus_tag="OO249_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5636795.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5363..8887
                     /gene="nifJ"
                     /locus_tag="OO249_002538"
     CDS             5363..8887
                     /gene="nifJ"
                     /locus_tag="OO249_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5636796.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DSYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9253..10386
                     /gene="ompN"
                     /locus_tag="OO249_002539"
     CDS             9253..10386
                     /gene="ompN"
                     /locus_tag="OO249_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR5636797.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10527..10961
                     /gene="uspF"
                     /locus_tag="OO249_002540"
     CDS             10527..10961
                     /gene="uspF"
                     /locus_tag="OO249_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5636798.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11518..12432
                     /gene="sitA"
                     /locus_tag="OO249_002541"
     CDS             11518..12432
                     /gene="sitA"
                     /locus_tag="OO249_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5636799.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12432..13259
                     /gene="sitB"
                     /locus_tag="OO249_002542"
     CDS             12432..13259
                     /gene="sitB"
                     /locus_tag="OO249_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5636800.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            13256..14113
                     /gene="sitC"
                     /locus_tag="OO249_002543"
     CDS             13256..14113
                     /gene="sitC"
                     /locus_tag="OO249_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5636801.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14110..14967
                     /gene="sitD"
                     /locus_tag="OO249_002544"
     CDS             14110..14967
                     /gene="sitD"
                     /locus_tag="OO249_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5636802.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         3839 a         3833 c         3696 g         3893 t
ORIGIN      
        1 agctacgagc ttttgcccgg cgagtttcgc gatatcatct acagacacat tattttgctt
       61 tgccagttgt tggtaactcg ctttacgttc ggcattaatg tcggttacta atttctctgt
      121 ttcagcatcc gttttcagcg caaccagata accataaaat gtttccccga cccgcccctg
      181 cgttctggct tcatccagag tcaatgccat tacattgctg cttaccagcc caacaagaaa
      241 cgcacaaaga agtaatgttt tcttcatcat cgcctcagta aagatcgcta cgagtttcaa
      301 gcagctcttc gacatctttg tctgccttga tgatgatctc atgctcaatt ttaacgttca
      361 tattgatagt gatcggttcc ttgggcgcag cgacttcaat acgcggcgta caggcagcca
      421 gcataaaaga tgacgtcaac gcagccagta aaattttcat tgtttttcct cacactcttt
      481 tccttgcgga cagtcatttc ccggcagacg tgcgttctgc tccaaccatg cctggagatt
      541 atcgccaaag cgtaaactgc gccacagcgt aaaaatattc tcttcatgat ggtaattaag
      601 gtttaccgta ccgcttttac catcaacgcg actggtacct gtaatgttgg cctgcatggt
      661 taataaaccg agattatcta aattaatatt tgtcgatgaa cggctaattt ccatatagcg
      721 caaccagtta attgctgaac ccgcagtcat attgtctttc accaccgcat ctgcggtgtc
      781 tttatccagc cgcaaggtca ttggcccgct atttgccagc caaccatctt tcactatcca
      841 tttttcgttg tttagccata acggcaatgc accactaaat gccccggaca tggcgaattg
      901 tttcggattg acggcgctaa ccagttcgct ggatgagaga ttattcagcc gcaacagcgc
      961 cgggtcatgt tgcggcatac gtaattgttt catcagtacg ttaccgccta acacatcgac
     1021 gctaacatca gtcaacagca agggttcttc ttcagtccac ggataacgcc cttgcaaatc
     1081 agccgtaata tttttcgctg tcaccagatt aatcacttcg gcaattcgca acgtaacggg
     1141 gccgcgagtc cccagatgcc aggctccatc ggcaaaacgg aaaggcagga caaaatcgac
     1201 accgttaacc tgattatctg gcatccaggc actaccgcct ttcaacacgc cgtgccctcc
     1261 cgcgcggaat ccttgttcag gcgcagcaga aaatgcaacc tgagcataca gttcaccatc
     1321 gcgtaagttc atcttccagt cgggtggcac cagcggctgg aatacagtca gtgattgttt
     1381 tggccaccag gcgttaccgc gcagacgaat accgtcccag cgaccattta cccgaaccgg
     1441 gccaatctca ccagcatgta aatcgccttt aaagaggaaa tacgtcggat cgcgcccatc
     1501 gacgctaaat tttaaggttg atggcggtaa cacactgccg ccagtgaaca gcgtttgccc
     1561 ggcgtccagt gacagcgcgc cgctgaagga gggatgctgt gcgtcacgta cccagacgac
     1621 gggcttgtcc agaattaatc gcggcttttc taccgtcatc gtaccgtatt gtaactggtc
     1681 gaaaccggtg gagaggtcgg tcagcgtaat cgtgctgtca tgccattcac cttttcctgc
     1741 gacatcccag gtggcattca tcggtgtaaa actgcctttt ccccagtagc gccactgcca
     1801 gcggcccgcg tcagggagaa aatcattcgc cagcccatcc atatgcagca cgaaatcgcc
     1861 cagttcattt tcatgcgcct gcaaaatggc ctgcaaacgt ccgtcaacac cacgttgggt
     1921 gacttttaca cccgctaaag gccagcggat ttcatcgatg tccagcgaat cgatgactct
     1981 tccctttgaa cgaagtaacg cgcctggctc aaaggccagc gttgggtcag tcagacttcc
     2041 acttagctgc gcgggtaagc gggcatataa aatgaggtcc gcctgtttcg cttcaccggt
     2101 cagctgcaga ggcagctggc tgttatccat gcttaatttt cctgggccaa aatttagcac
     2161 cgcgttgcct ttacccgctt gcccctgggt cagcacactc agtcgtccgc tgatcagagc
     2221 gttctcaagc cctgcctgcc agttgtcgac tttgacaccc agtcggccac tcaaaggaaa
     2281 acctgcatac ggccaactcc agcgaccatc gcttacggtc aattgttgac gagtaatttg
     2341 ccacggcaaa tcgagcaacg gatcgccgtt atcacgtgcc agcacaatca attgcccgct
     2401 attttcctgc cagtccagct ctgcatccac cagtgacggt tcctgcggca agtttaacgt
     2461 cgcagtagca tgcccactta caggaagtcc atccggcacg agcggcatag taaattcccc
     2521 caccagttta accggcggct gatattcaaa cgcgatgaca tccagttcgc tgactgtaag
     2581 ttgttgccct ttcagctggc cttgaaattt aactttttcg ccctgataac gcagttgctg
     2641 gatatcagag gttaatgcga gagagagttt tccctgccat tcctgccagg gagaaaaaat
     2701 cagtttatcg atattgatcc aggtgttagg cagcatggac tgccactgcg cgagggtttt
     2761 tggagcagct ggcgattgct ccgtctgcgg caatttcgcc agacaagcag aatcaagttc
     2821 taccgtgccg acgttcaata accagcggct gggatgtgaa aggctggcgt tggtgatatg
     2881 cgcaagctga caatctccca ccagataacg gagatcggga atgattaaac ctttacgcgt
     2941 aatgcgtggg ctttcatcta atgcaatacg tgtcccgagc ggtagccaga tgcccgccag
     3001 cgtgggaacc cacagcccga gcgtcatcag cagcgtcaac ggcacaagaa taatcagtaa
     3061 taacagcgcg agaacggctt tatatttacc cagcatgggt agttaatatc ctgatttagc
     3121 gaaaaattaa gcattcaata cgggtattgt ggcatgttta accgttcagt tgaaggttgc
     3181 gcctacacta agcatagttg ttgatgaatt tttcaatatc gccatggttt tcaattatat
     3241 ttgaaatttt gtaaaatatt tttagtggct taaatgtgat tcaacatcac tggagaaagt
     3301 cttatgaaac tcgccgttta tagcacaaaa cagtacgaca agaagtacct gcaacaggtg
     3361 aacgagtcct ttggctttga gctggaattt tttgactttc tgctgacgga aaaaaccgct
     3421 aaaactgcca atggctgcga agcggtatgt attttcgtaa acgatgacgg cagccgcccg
     3481 gtgctggaag agctgaaaaa gcacggcgtt aaatatatcg ccctgcgctg tgccggtttc
     3541 aataacgtcg accttgatgc ggcaaaagaa ctggggctga aagtagtccg tgttccagcc
     3601 tatgatccag aggccgttgc tgaacacgcc atcggtatga tgatgacgct gaaccgccgt
     3661 attcaccgcg cgtatcagcg tacccgtgac gctaacttct ctctggaagg tctgaccggc
     3721 tttactatgt atggcaaaac ggcaggcgtt atcggtaccg gtaaaatcgg tgtggcgatg
     3781 ctgcgtattc tgaaaggttt tggtatgcgt ctgctggcgt tcgatccgta tccaagtgca
     3841 gcggcgctgg aactcggtgt ggagtatgtc gatctgccaa ccctgttctc tgaatcagac
     3901 gttatctctc tgcactgccc gctgacaccg gcaaactacc atctgttgaa cgaagccgcc
     3961 ttcgatcaga tgaaaaatgg cgtgatgatc gtcaatacca gtcgcggtgc attgattgat
     4021 tctcaggcag caattgaagc gctgaaaaat cagaaaattg gttcgttggg tatggacgtg
     4081 tatgagaacg aacgcgatct gttctttgaa gataaatcca acgacgtgat ccaggatgac
     4141 gtattccgtc gcctgtctgc ctgccacaac gtgctgttta ccgggcacca ggcattcctg
     4201 acagcagaag ctctgaccag tatttctcag actacgctgc aaaacttaag caatctggaa
     4261 aaaggcgaaa cctgcccgaa cgaactggtt taatgttgca gctcccctgc aatccagggg
     4321 agctgattca gataatcccc aatgaccttt cattctctat tcttaaaata gtcctgagtc
     4381 agaaactgta attgagaacc acaatgaaga aagtagccgc gtttgttgcg ctaagcctgc
     4441 tgatggcggg atgtgtaagt aatgacaaaa ttgctgttac gccagaacag ctacagcatc
     4501 atcgctttgt gctggaaagc gtaaacggta agcccgtgac cagcgataaa aatccgccag
     4561 aaatcagttt tggtgaaaaa atgatgattt ccggcagcat gtgtaaccgc tttagcggtg
     4621 aaggcaaact gtctaatggt gaactgacag ccaaagggct ggcaatgacc cgtatgatgt
     4681 gcgctaaccc gcagcttaat gaactcgata acaccattag cgaaatgctg aaagaaggtg
     4741 cacaagtgga tctgaccgcg aaccagttaa cgctggcgac cgcgaaacag acattaactt
     4801 ataagctggc ggatttaatg aattaatagc tgccacagct cccggcggca agtgactgtt
     4861 cgctacagcg tttgccgttg ggtaatgcac acatcccaat cgccgtgcca tccagttgac
     4921 gggcaacaga aagcgaaccg ccgatcattg cacaatttgc ttctccacta ctggacatcg
     4981 acgcttttaa acctggcgct acgtgcgccg cagtcgcctg ctgaacaggt tcactactac
     5041 acgccgacaa caataaagcg gcacacccta cccaaaacgc tgctcgcatc ttttcttcct
     5101 ctgatcttca agccaaacga caccgccata aataataggc agcacagagg gcgacatcga
     5161 gagctgtcct gcgcgttgcc ccgcattttt acttttttat ggctattttt ttgccctctg
     5221 tttgatcaaa acattcatta cgctgatgtg ggggacacaa aagcgaaaat gcagaagaaa
     5281 gccatttgct aaaattgaaa gattactact gggcgcgcag caatttcgtg cgcccctcat
     5341 ttgcgcaatg taagggtgtc atatgattac tattgacggt aatggcgcgg ttgcttcggt
     5401 ggcgtttcgc accagtgaag ttatcgccat ctaccctatt acccccagtt ccacgatggc
     5461 agaacaggct gatgcctggg ccggaaacgg cttgaagaac gtttggggag acacaccacg
     5521 cgtggttgaa atgcagtcgg aagcgggtgc tatcgctacc gtgcatggcg ctttgcagac
     5581 gggtgccctt tcaacatcgt ttacgtcatc gcagggtttg ctgctgatga tcccgacact
     5641 gtacaaactg gcaggcgaac tcacaccgtt tgtcctgcat gtagcggcac gtaccgttgc
     5701 cacacatgca ctctctattt ttggcgatca ttccgacgtc atggcggtgc gccagacggg
     5761 ttgcgcgatg ttgtgtgcgg caaacgtcca ggaagcgcaa gactttgctc tcatttcgca
     5821 catcgcgacg ctgaaaagcc gcgtgccatt tattcatttc tttgatggtt tccgcacgtc
     5881 ccacgaaatc aataaaattg tcccgctggc cgatgacacg attcttgatc tcatgccgca
     5941 ggccgaaatt gatgctcatc gcgcccgggc gctcaacccg gaacatccgg tgatccgtgg
     6001 tacgtccgcc aatcctgact cttatttcca gtctcgcgaa gccaccaacc cctggtacaa
     6061 cgcagtctat gaccatgttg aacaggcgat gaatgatttc tctgccgcga caggtcgtca
     6121 gtatcagccg tttgaatatt acgggcatcc gcaagctgaa cgggtgatta tcctgatggg
     6181 ctcagccatt ggcacctgtg aagaagtggt tgatgaattg ctaacccgtg gcgaaaaagt
     6241 cggcgtgctg aaagttcgcc tgtaccgccc cttctcagcc aaacacttgc tacaaactct
     6301 accgggatcc gtacgcagcg tggcggtact ggacagaacc aaagaacccg gtgcccaggc
     6361 agaaccgctc tatctggatg tgatgaccgc actggcagaa gcctttaata atggcaagcg
     6421 cgaaactctg ccccgtgtca ttggtgggcg ctatggtctt tcatccaaag aatttggccc
     6481 ggactgtgta ctggcggtat ttgccgagct caacgcggct aaaccgaaag cgcgctttac
     6541 ggttggtatt tacgatgatg taaccaatct gtccctgccg ttgccggaaa acacccagcc
     6601 aaactcggcg aaacttgaag ccttgtttta tggccttggt agtgatggca gcgtttccgc
     6661 gaccaaaaac aatatcaaga ttatcggtaa ttccacgccg tggtacgcac agggctattt
     6721 tgtttacgac tccaaaaaag cgggcggcct gacggtttct caccttcgcg tgagcgaaca
     6781 gccgattcgt tccgcttatc tcatttccca ggctgatttt gttggctgcc accagttgca
     6841 gtttatcgat aaatatcaga tggctgagcg tttaaaacct ggcggcattt tcctgctcaa
     6901 cacgccgtac agcgcagatg aagtgtggtc gcgcttgccg caagaagttc aggccgtgtt
     6961 aaaccagaaa aaagcgcgct tctatgtgat taacgcggcg aaaatcgccc gcgaatgtgg
     7021 cctggcggcc cgtattaata ccgtcatgca gatggctttt ttccatctga cgcaaattct
     7081 gcctggcgat agcgccctcg cagaattgca gggtgcgatt gccaaaagtt acagtagcaa
     7141 aggccaggat ctggtggaag gcaactggca ggctctggcg ctggcgcgtg aatccgtaga
     7201 agaagttccg ttgcaaccgg taaatccgca cagcgccaat cgaccgccag tggtttccga
     7261 tgccgcccct gatttcgtga aaaccgtaac cgcagcgatg ctcgcagggc ttggtgatgc
     7321 cctccccgtt tcggcgctgc cgccagacgg cacctggccg atggggacca ctcgctggga
     7381 aaaacgcaat atcgccgaag agatccccat ctggaaagag gaactctgta cccaatgtaa
     7441 ccactgcgtc gccgcttgcc cacactcagc tattcgcgca aaagtggtac cacccgaagc
     7501 gatggaaaac gcccctgcca gcctgcattc gctggatgtg aaatcgcgtg atatgcgcgg
     7561 gcagaaatat gtcttgcagg tggctccgga agattgcacc ggttgtaacc tgtgcgtcga
     7621 agtttgcccg gcgaaagacc gtcagaatcc agagattaaa gccatcaata tgatgtcgcg
     7681 cctggaacat gtcgaagaag agaaaatcaa ttacgatttc ttcctcaacc tgccagaaat
     7741 cgaccgtagc aaactggaac gtattgatat tcgtacatcg cagctgatta caccgctgtt
     7801 tgaatattcc ggtgcttgct ccggttgtgg cgagacgccg tatattaaat tgctgactca
     7861 gctctatggc gaccggatgt tgatcgctaa cgccaccggc tgttcttcaa tttatggcgg
     7921 taacctgccc tctacaccgt ataccaccga tgccaacggt cgtgggccgg catgggcgaa
     7981 ctctctattt gaagataatg ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca
     8041 ccgtgtccgc gtgctgcgtc tgctggatca atttgccgat aaaatcccgg cggaattact
     8101 gacggcgttg aaatcagacg ctacgccaga ggttcgtcga gaacaggtcg cagctttacg
     8161 ccagcaactc aacgatgttg ccgaagcaca tgaactgcta cgtgatgcag atgcactggt
     8221 ggaaaaatca atctggctga ttggtggtga tggctgggct tacgatatcg gctttggcgg
     8281 tctggatcat gtattgagtt tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg
     8341 ctattccaac accggtggtc aggcgtcgaa agcgacaccg ctgggtgcag taactaaatt
     8401 tggcgagcac ggcaaacgta aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg
     8461 tcatgtttat gtggcgcaga tttctctcgg cgcgcagctg aatcagacgg tgaaagcgat
     8521 tcaggaagcg gaagcgtatc cgggaccatc gctgatcatt gcttatagcc cgtgtgaaga
     8581 gcatggttac gatctggcac tcagccatga ccagatgcgc caactcacag ctaccggctt
     8641 ctggccgcta tatcgcttcg atcctcgtcg tgccgatgaa ggcaaactgc cgctggcgct
     8701 ggattcacgc ccgccgtcag aagcactgga agaaacgtta cttcacgagc aacgtttccg
     8761 tcgtctgaat tcgcagcagc cagaagtggc agaacagtta tggaaagatg ctgcagctga
     8821 tttgcaaaaa cgctatgact tcctggcaca aatggccgga aaagcggaaa aaagcaacac
     8881 cgattaaatg ctctggataa ggattatcca attctaaaaa aaagcccgga cgactgttcg
     8941 ggcttgtctt tttatatgtt ggaaaatcag tggcaatgca atggcccaac agaaaataaa
     9001 atctgtgaca ataaaggcat ataacccgcg cagaataacg tatagaaaat taaaagtata
     9061 atttttattt tgtatgtatt atcttcgata cattcaccag acttattatc atttcagaag
     9121 aattatttga ttaaggtttt acttaaggcg taacaaatga tatttatcgg ctaactgaac
     9181 ttctccttta ggatgttttc actccccgga atggggataa aaggcaaata aaataactaa
     9241 aggatttatt caatgaaaag caaagtactg gcacttttaa ttcctgccct gctcgccgca
     9301 ggtgctgcac atgcagccga agtttataat aaagacggca acaaattaga tctgtatggc
     9361 aaagttgatg gcctgcatta tttttctgat aattcagcga aagatggcga tcagagctat
     9421 gcgcgtctgg gttttaaagg cgaaacccaa attaacgatc aactcactgg ctacggtcaa
     9481 tgggaataca atattcaagc aaacaacact gaatcttcaa aaaaccagtc atggacccgt
     9541 ctggcatttg ccgggctgaa atttgcagat tacggttctt tcgattacgg acgtaattat
     9601 ggcgtaatgt acgacatcga aggctggacc gatatgctgc ctgaatttgg cggtgactct
     9661 tataccaatg cagacaactt tatgactggt cgagccaatg gcgtcgcgac ttatcgtaat
     9721 actgatttct tcggtctggt aaatggtctg aacttcgcgg tgcagtatca aggtaacaac
     9781 gaaggagcca gtaatggtca ggaaggcacc aacaacggac gtgatgttcg ccatgaaaac
     9841 ggtgacggct ggggtttttc cacaacatat gatttaggca tgggctttag cgctggtgcg
     9901 gcatacacct cttctgaccg caccaatgac caggttaacc atactgcggc gggtggtgat
     9961 aaagcagacg cgtggactgc tgggctaaaa tacgatgcta acaatattta cctagcaacc
    10021 atgtattcag aaacgcgtaa tatgaccccg tttggcgaca gcgattatgc tgtggcaaac
    10081 aaaacccaga attttgaagt cactgcccag taccagtttg attttggtct gcgtccggca
    10141 gtctctttcc tgatgtctaa aggccgtgac ctgcacgctg ccggtggtgc agacaacccg
    10201 gcaggtgttg atgataaaga tctggttaaa tacgccgatg ttggcgcgac ttactatttc
    10261 aataaaaaca tgtccaccta cgttgactat aaaatcaacc tgttggatga agatgacagc
    10321 ttctacgctg ccaatggcat ctctaccgat gatattgtcg ctttaggtct ggtttatcag
    10381 ttctaaatcc tcctgcccgc tgttatggcg ggctttttct gcttattctt cctcctttga
    10441 tctaaattaa aaatgtgaac tccatcatta cacaaaaagt gtcatctggc gttacacttt
    10501 atgcggatac taaaacagga ggttttatga acagaacgat tcttgtccct atcgatattt
    10561 ccgattcaga attaactcaa cgcgtgatta gccacgttga ggcagaggca aagattgacg
    10621 atgcagaggt tcatttcctg acggtaatac cttcactgcc ctactatgcc tctctgggtt
    10681 tagcgtattc cgcagaatta ccggcaatgg atgacctgaa agcggaagcc aaatcgcaac
    10741 tggaagagat cattaaaaaa tttaaactgc caaccgacag agtgcatgtc catgttgagg
    10801 aaggctcgcc caaagaccgc attctggaat tggcgaagaa gatccccgct catatgatca
    10861 tcattgcttc ccatcgaccg gatatcacca cttatctgct cggttccaac gccgcagctg
    10921 tagtgcgtca cgcagagtgc tccgtgctgg ttgtgcgctg acactaacgc ccgcacattg
    10981 ctgcgggctt tttgattcat ttcgcaaatg tgctgacatt ttcccctcta atccgtacca
    11041 tacacgccac tgtttttata tcagatttct tatgctgggc attccggcat gacgcatact
    11101 cttctgatgc catataacga attgagtcgc ttttaaatgt cgcaaaatca agaaattagt
    11161 aagaaagaac aatacaacct gaacaagtaa gggcaaaaat cacaactatc tgactcataa
    11221 gtattttatt tatttttcaa tatgttaaac attatcagcg acaacaataa gctacgataa
    11281 cccactcttt ttttgcccca tttttgccat caacaatttt cctcataaag tcatttcaaa
    11341 gcctaatagc tgctctactt ttttcctcaa tatcatgtcg ccagcaggaa acaacctaat
    11401 ccctgtttta ggcctcaaag ctgttaatga taattatttt cattttgtat aggttatgct
    11461 ataaaacata gccgttgcta tgtttgttct aattttgctc actataggta ctaaattatg
    11521 cactcaataa aaaaagtaac catgctcttg gggtgcctcg ccctcacctg ctcgatcgca
    11581 tttcaggcaa gtgcaactga aaaattcaag gtcattacaa catttaccat catcgcggat
    11641 atggccaaaa acgtggctgg agatgctgca gaagtctcat ccataaccaa gcctggtgca
    11701 gaaattcatg agtatcagcc tacccctggc gatattaaac gtgcacaggg ggcacaactg
    11761 attctcgcca atggtatgaa tctggaattg tggttccaac gcttttacca gcatctcaat
    11821 ggggttccag aagtaattgt ctcttcgggt gtgacgccag tagggatcac cgaaggaccc
    11881 tatgagggca aacctaatcc ccatgcatgg atgtcgccag ataatgctct gatttacgtc
    11941 gataatattc gtgatgcgtt gataaaatac gatccggcaa atgcacaaac ctaccaacgt
    12001 aatgccgata cttataaagc caagattacc caaacccttg cccccctgcg taagcagatt
    12061 acggaactcc ctgagaatca gcgatggatg gtcaccagtg aaggggcttt ttcttatctc
    12121 gcacgcgatt tggggctaaa agagctttat ctgtggccga ttaatgccga tcaacaagga
    12181 acaccgcagc aggtacgtaa ggttgttgat atagttaaga aaaatcatat cccggcagtc
    12241 tttagcgaga gtacgatttc cgataaacca gcgcgtcagg ttgcgcgtga aaccggcgca
    12301 cactacggcg gtgtgctcta tgtcgattcc ctgagcacag aaaacggccc ggtacctacg
    12361 tatatcgacc ttcttaaggt tactaccagt acgctggtac agggaattaa agccgggaaa
    12421 agggagaaat aatgatgcaa tctgcaggca ttgtcgttaa tgatgttact gtcacctggc
    12481 gtaacgggca cacagcactg cgcgatgcat ccttcacagt accaagtgga tctattgccg
    12541 ctctggttgg ggtaaacggt tccggaaaat cgacgttgtt taaagcgatt atgggatttg
    12601 tgcgtctgac cagcggaaag atatctgttc tgggtattcc cacgcgacag gcgctacaga
    12661 aaaacctggt tgcctacgtc cctcagtcag aagaggttga ctggtcattt cctgtactgg
    12721 tggaagatgt ggtgatgatg ggccgctatg ggtatatggg tatgttgcgt atagccaaaa
    12781 agagggatcg tcagattgtc accgatgcgc tggaacgtgt cgatatggtg gattttcgcc
    12841 atcgtcaaat cggcgagctt tccggtggac aaaagaaacg cgtctttctg gcgagagcga
    12901 ttgcacagca gggtgatgtg atcctgctgg atgaaccttt taccggcgtt gatgtgaaaa
    12961 ccgaagcgaa aatcatcagc ctgttgaggg agttacgcgc ggaaggcaaa acgatgcttg
    13021 tctcaacgca caatctcggc tcagtcacga cattttgcga ttatacggtc atggtcaaag
    13081 gcaccgtcct ggcaagcggg ccgacagaca ccacttttac agccgagaac ctggagctgg
    13141 cttttagcgg cgtactgcgc catgtcacac ttaacggctc ggaagaaagc attattactg
    13201 acgatgaacg cccttttgtg gcacatcgac cgtcagcggt gcagagggaa gcaagatgaa
    13261 cgtgctactg gaacccttca gctatgaata tatgctcaat gcgatgtggg tctcggcaat
    13321 ggtcggcggt ctctgcgctt tcctgtcatg ctatctgatg ctaaaaggct ggtcgctgat
    13381 tggcgatgcg ctgtcgcact ctattgtccc gggtgttgcg ggggcatata tgctggggct
    13441 tccgttttct ctgggagcct tcttttccgg cggactggca gcgggcagca tgctgtttct
    13501 taaccaacgc acccgcttaa aagaagatgc catcatcggc ctgatcttct cgtccttttt
    13561 tggcctgggg ctgtttatgg tgtcattgaa cccgacatcc gtgaacattc agaccatcgt
    13621 cctgggcaat attctggcaa tcgatccggc ggatatcctg caactcacta tcattggcat
    13681 tctctcgatc atcgtgcttt ttttcaaatg gaaagatctt atggtgacct ttttcgacga
    13741 gaatcacgcc cgtgctattg gattacatcc gggcagatta aaaatcctct tcttcaccct
    13801 gctgtcggtt tcgaccgttg cggcgctgca aacggttggt gcctttctgg tgatctgtct
    13861 ggtggtgact cccggtgcaa cagcatggtt gttaaccgat cgttttcccc gcctgctcat
    13921 aattgctgta actattggca gtgtaaccag tttcctcggc gcgtgggtca gttactttct
    13981 ggacggcgcg acaggtggaa tcatcgtggt ggcgcagaca ctgctgtttc ttttagcatt
    14041 tgtatttgcg cccacccatg gacttttggc aaatcgtcgg cgtgcgcata aagcgctgga
    14101 ggatcgctca tgatggcatt attactggag ccactgcagt tcacctttat gagtcacgca
    14161 ctgctgattt cactggtagt ttccataccc tgtgcgctgc tgtcggtctt tctggtcctg
    14221 aaaggttggg cactgatggg tgacgcgatg agtcatgcgg tttttcctgg catcgtgctt
    14281 acctggattc tggggttacc tctggctaca ggggcattcg tcgccggagt attctgcgcg
    14341 gtggcgacgg gatacctgaa agacaacagc cgaatcaagc aagatacagt tatggggatt
    14401 gttttttctg gcatgtttgc cgcaggtctg atcttgtata ttgcaatcaa accagacgta
    14461 catcttgacc atattctttt cggcgacatg ctcgggataa ccatcggcga tataatccag
    14521 acggtgatta ttgccgggct ggttacactt gttattagcg taaaatggcg ggattttttg
    14581 ctgttcagct ttgattatca acaggcgcag gtaagcggtt tgcatacgag atggctgcat
    14641 tatgggctgc tgtgcatggt ttccctgacc attgtggcga cgctgaaagc agtaggtatc
    14701 attctctcta tttctctgct cattgcgcct ggcgccattg cagtactcct gacacaacgc
    14761 tttcatattg cgctcctact ggcgactggc atatcggtaa tagtgtcaat gactggtgtc
    14821 tggctgtcct tttttatcga cagcgcacca gctccgacga ttgtcgtctt attcgcggtc
    14881 gtgttcatca tgacgtttac cgttaccagc atcaacgcac gcacaaaggg aaacgcccat
    14941 acacaggatc tgttatcacc caattagcac aatatccgag gacagcatcg tccgccagcg
    15001 tccgccagcc tacagaagtt cggaaagcca tggaaggtcg ttgggaggag gtacgaattc
    15061 tatcatgcaa aaaatacgta aaatcgataa cgcctagaaa tcattcaata ctcgcactat
    15121 cgaaagttta ccagccaacc gcggcacgtt cctgcatacg acgtgccgca gcttctgtta
    15181 ttccacgata aaattcctaa ccgaattcaa atccttcaga ctattcagct cttccttcag
    15241 ctcccgctga cggcgataaa t
//
LOCUS       DAJTOR010000093        14936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTOR010000093 DAJTOR010000000
VERSION     DAJTOR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.093"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..450
                     /locus_tag="OO249_002545"
     CDS             <1..450
                     /locus_tag="OO249_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR5636803.1"
                     /translation="QEVNAALFKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIA
                     DPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMT
                     LGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(491..1423)
                     /locus_tag="OO249_002546"
     CDS             complement(491..1423)
                     /locus_tag="OO249_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5636804.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            1787..4573
                     /gene="polA"
                     /locus_tag="OO249_002547"
     CDS             1787..4573
                     /gene="polA"
                     /locus_tag="OO249_002547"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5636805.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNPLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(4955..5587)
                     /locus_tag="OO249_002548"
     CDS             complement(4955..5587)
                     /locus_tag="OO249_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5636806.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRHSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6169..6678
                     /gene="yihI"
                     /locus_tag="OO249_002549"
     CDS             6169..6678
                     /gene="yihI"
                     /locus_tag="OO249_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5636807.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            6867..8240
                     /gene="hemN"
                     /locus_tag="OO249_002550"
     CDS             6867..8240
                     /gene="hemN"
                     /locus_tag="OO249_002550"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5636808.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8430..8540)
                     /locus_tag="OO249_002551"
     CDS             complement(8430..8540)
                     /locus_tag="OO249_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5636809.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8651..10060)
                     /gene="glnG"
                     /locus_tag="OO249_002552"
     CDS             complement(8651..10060)
                     /gene="glnG"
                     /locus_tag="OO249_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5636810.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKKIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10072..11121)
                     /gene="glnL"
                     /locus_tag="OO249_002553"
     CDS             complement(10072..11121)
                     /gene="glnL"
                     /locus_tag="OO249_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5636811.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11295..12704)
                     /gene="glnA"
                     /locus_tag="OO249_002554"
     CDS             complement(11295..12704)
                     /gene="glnA"
                     /locus_tag="OO249_002554"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5636812.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13077..14900
                     /gene="typA"
                     /locus_tag="OO249_002555"
     CDS             13077..14900
                     /gene="typA"
                     /locus_tag="OO249_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5636813.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
BASE COUNT         3864 a         3745 c         3850 g         3477 t
ORIGIN      
        1 caagaagtga atgctgcatt gttcaaagag tatttaccgt tattacaaca aagtgagccg
       61 accattaaac aaccggtaag atggaagaac gcactcggcg aactaaatgc caatctggat
      121 atcagtattg ccgacccagc caaatcttca tcatccacaa acaaagatat caaatcgctc
      181 aattttgatg tgaagttacc gcttaatgtc gtcacagaaa ccgcaaaaca gcttaattta
      241 tctgaaggaa tggatgcgga aaaagcgcaa aagcgggctg ataaacaaat cagcgggatg
      301 atgaccttag gtcagatgtt tcagttaatc acgattgaca acaataccgc ctcgctgcaa
      361 ctgcgttata caccgggtaa agttgttttt aacggacagg agatgagcga agaagaattt
      421 atgtctcgtg ccggacgttt tgttcattaa aatactcact cgcctctgct cattcagagg
      481 cgagcaaaat ttacttgtcc tgccgctgtg atgacatcaa cgacgtcaga agtcggtctt
      541 tttcctgcca cagtgagttc agccattgct gaaaatggcg cttaaagctt ttgtcattga
      601 tgtagtcgcc atgtaactca tcggcaatag gctgtaaatc cacatgaacg acaattcgcg
      661 tcagtttacc gcttaacata tcgaagaacg gctgacggtt attgtccgga taacacagcg
      721 taacattaag cagtttatcg aattgtttac ccagtacatt tagcgccatc gcaatgcctg
      781 ccgcctttgg tggcaacaag ttttgaaaag tggagtgggt ttgctgatgt ttttcttgcg
      841 tgaaacggga gccttcaacg aaattgacaa tggtggtggg atgcaggcga aacttttcac
      901 aagaacgccg agtggtttca acatctttac cacgtcgctc cggatgacgt aataaataag
      961 cgcgggaata acgcttcata aacggcatat ccagcgccca gcacgccagg ccaagaaatg
     1021 gcacccaggc cagctgttgc ttgaggaaat atttattcat cggaatgtgc ttacgaaaca
     1081 gcacgcacag tacgacaata tctgcccaac tacggtggtt acaaataagc agataccagt
     1141 tcttcttact taacccttcc agcccgtgaa cttcccattg caggtgtggg ttcagatgca
     1201 gtaataccgc cagaccttca caccagcaat acatcataaa atcacaaaaa cgcgaaacct
     1261 ttcgccaaat gactggcaca ggtagcaata gttttacaat cccggcaatg atgatcggga
     1321 cggaacaaaa aatagtgacc aaaatggtca atacgatact cagcaaaagg gttatcgcag
     1381 cgagtattct cgtcataatg aatttattca aaaggttagc catagacagt gcgctaaaga
     1441 agcgcaaggc gcagatttta gcagaaaacg acccaaataa cggatgatcc ttaaggagaa
     1501 aaataattca tatctatcca cattagaaaa aatcccatta tctcaattat tagggatggc
     1561 tttattttta actacatgaa aaacaaagac aaacatcata ctgtaaaaag catgataata
     1621 aattaaaagc gatgtaaata atttatgcac aaagttatcc acatgacgat ttgcgagcga
     1681 tccagaagat ctacaaaaga ttttcacgaa aagcggtgaa aaactcatgt ttttatcctg
     1741 tctgtggcat cctttaccca taatctgata aacaggcacg gacattatgg ttcagatccc
     1801 ccaaaatcca cttatccttg tagatggttc atcttatctt tatcgcgcat atcacgcgtt
     1861 tcccccgctg actaacagcg caggcgagcc gaccggtgcg atgtatggtg tcctcaacat
     1921 gctgcgcagt ctgatcatgc aatataaacc gacgcatgca gcggtggtct ttgacgccaa
     1981 gggaaaaacc tttcgtgatg aactgtttga acattacaaa tcacatcgcc cgccaatgcc
     2041 ggacgatctg cgagcacaaa tcgaacctct gcacgcgatg gttaaagcga tgggactgcc
     2101 gctgctggcg gtttctggcg tagaagcgga cgacgttatc ggtactctgg cgcgcgaagc
     2161 cgaaaaagcc gggcgtccgg tgctgatcag cactggcgat aaagatatgg cgcagctggt
     2221 gactccaaat attacgctta tcaataccat gacgaatacc atcctcggac cggaagaggt
     2281 ggtgaataag tacggcgtgc cgccagaact gatcatcgat ttcctggcgc tgatgggtga
     2341 ctcctccgat aacatccctg gcgtaccggg cgtcggtgaa aaaaccgcgc aggcattgct
     2401 gcaaggtctg ggcgggctgg atacgctgta tgccgagcca gaaaaaattg ctgggttgag
     2461 cttccgtggc gcgaaaacaa tggcagcgaa gctcgagcaa aacaaagaag ttgcttatct
     2521 ctcataccag ctggcgacga ttaaaaccga cgttgaactg gagctgacct gtgaacaact
     2581 ggaagtgcag caaccggcag cggaagagtt gttggggctg ttcaaaaagt atgagttcaa
     2641 acgctggact gctgatgtcg aagcgggcaa atggttacag gccaaagggg caaaaccagc
     2701 cgcgaagccg caggaaacca gtgttgcaga cgaagcgcca gaagtgacgg caacggtgat
     2761 ttcttatgac aactacgtca ccatccttga tgaagaaaca ctgaaagcgt ggattgcgaa
     2821 gctggaaaaa gcgccggtat ttgcatttga taccgaaacc gacagccttg ataacatctc
     2881 tgctaacctg gtcgggcttt cttttgctat cgagccaggc gtagcggcat atattccggt
     2941 tgctcatgat tatcttgatg cgcccgatca aatctctcgc gagcgtgcac tcgagttgct
     3001 aaaaccgctg ctggaagatg aaaaggcgct gaaggtcggg caaaacctga aatacgatcg
     3061 cggtattctg gcgaattacg gcattgagct gcgtgggatt gcgtttgata ccatgctgga
     3121 gtcctacatt ctcaatagcg ttgccgggcg tcacgatatg gacagcctcg cggaacgttg
     3181 gttgaagcac aaaaccatca cttttgaaga gattgcgggt aaaggcaaaa atccactgac
     3241 ctttaaccag attgccctcg aagaggccgg acgttacgcc gccgaagatg ctgatgtcac
     3301 cttgcagttg catctgaaaa tgtggccgga cctgcaaaaa cacaaagggc cgttgaacgt
     3361 cttcgagaat atcgaaatgc cgctggtgcc ggtgctttca cgcattgaac gtaacggtgt
     3421 gaagatcgat ccgaaagtgc tgcacaatca ttctgaagag ctcaccttgc gtctggctga
     3481 gctggaaaag aaagcgcatg aaattgcagg tgaagaattt aacctttctt ccaccaagca
     3541 gttgcaaacc attctgtttg aaaaacaggg tattaaaccg ctgaagaaaa cgccgggtgg
     3601 cgcgccgtca acgtcggaag aggtactgga agaactggcg ctggactatc cgttgccaaa
     3661 agtgattctg gagtatcgtg gtctggcgaa gctgaaatcg acctacaccg acaagctgcc
     3721 gctgatgatc aacccgaaaa ccgggcgtgt gcatacctct tatcaccagg cagtaactgc
     3781 aacgggacgt ttatcgtcaa ccgatcctaa cctgcaaaac attccggtgc gtaacgaaga
     3841 aggtcgtcgt atccgccagg cgtttattgc gccagaggat tatgtgattg tctcagcgga
     3901 ctactcgcag attgaactgc gcattatggc gcatctttcg cgtgacaaag gcttgctgac
     3961 cgcattcgcg gaaggaaaag atatccaccg ggcaacggcg gcagaagtgt ttggtttgcc
     4021 actggaaaca gtcaccagcg agcaacgccg tagcgcgaaa gcgatcaact ttggtctgat
     4081 ttatggcatg agtgctttcg gtctggcgcg gcaattgaac attccacgta aagaagcgca
     4141 gaagtacatg gacctttact tcgaacgcta ccctggcgtg ctggagtata tggaacgcac
     4201 ccgtgctcag gcgaaagagc agggctacgt tgaaacgctg gacggacgcc gtctgtatct
     4261 gccggatatc aaatccagca atggtgctcg tcgtgcagcg gctgaacgtg cagccattaa
     4321 cgcgccaatg cagggaaccg ccgccgacat tatcaaacgg gcgatgattg ccgttgatgc
     4381 gtggttacag gctgagcaac cgcgtgtacg tatgatcatg caggtacacg atgaactggt
     4441 atttgaagtt cataaagatg atgttgatgc cgtcgcgaag cagattcatc aactgatgga
     4501 aaactgtacc cgtctggatg tgccgttgct ggtggaagtg gggagtggcg aaaactggga
     4561 tcaggcgcac taagattcgc ctgaacatgc ccttttttcg taagtaagca acataagctg
     4621 tcacgttttg tgatggctat tagaaattcc tatgcaacaa ctgaaaaaaa attacaaaaa
     4681 gtgctttctg aactgaacaa aaaagagtaa agttagtcgc gtagggtaca gaggtaagat
     4741 gttctatctt tcagaccttt tacttcacgt aatcggattt ggctgaatat tttagccgcc
     4801 ccagtcagta atgactgggg cgttttttat tgggcgaaag aaaagatccg taatgcctga
     4861 tgcgctatgt ttatcaggcc aacggtagaa ttgtaatcta ttgaatttac gggccggata
     4921 cgccacatcc ggcacaagca ttaaggcaag aaaattattc gccgtcctgc gtttcttcta
     4981 caggctgcat ctcgctaaac caggtatcca gtttctgccg cagcttgtcc acgccttgtt
     5041 tcttcaacga agaaaacgtt tcaacctgca catcaccgtt aaacgccagt acagcttcac
     5101 gcaccatatt caattgcgct ttacgtgcgc cgcttgccag tttgtccgct ttggtcagca
     5161 gcaccagaac ggcgatattg ctgtctaccg cccactcaat catctgctga tccaaatctt
     5221 tcagcggatg gcgaatatcc attagcacca ccagaccttg caggctatga cgtttttcga
     5281 ggtattcgcc gagcgcacgc tgccatttgc gcttcatctc ttccgggact tccgcataac
     5341 cgtacccagg caagtcaacc agacgcttgc cgtcagccac ttcaaacagg ttgataagct
     5401 gggtgcgccc tggggttttt gaggtacgag ccaggctttt ctggttagtc agcgtgttca
     5461 gcgcgctgga tttacctgcg ttggaacggc ctgcaaaagc cacttcaatt ccggtatcgg
     5521 aaggtaggtg gcgaatatca ggcgcactca tcacaaaatg cgtctgttga taattcaaat
     5581 tagtcaaagc ggtcgtctcc gtcagtcaaa gctttgtggc gattatacct gaaccataat
     5641 aaaaggcgga tttttcggcg tgagcgttgt aagtaaaaac catacgcttt gtgagacatt
     5701 gccgatagtc tttatgcgaa atagcagaga aaattctgcg atgcatgtca aataagctat
     5761 ataaatcagt gaattgcttt tatataaaac tctgaaaatg tgcaaatact gatggcggtt
     5821 gattgtttgt ttaaagcgaa ggcgtaaagt agcacccata gagcgaggac gctaacagga
     5881 acaatgactc aggatgaggg tcaggagcgc caggaggcga agacagagga ttgtcaggaa
     5941 gacaaacgtc cggagacgta attaaacgga aatggaatca acacggattg ttccggctaa
     6001 aggaaaaaca gggtgtgttg gcggcctgca aggattgtaa gacccgttaa gggttatgag
     6061 tcaggaaaaa aggcgacaga gtaatctgtc gccttttttc tttgcttgct ttctgttaga
     6121 ttccgccgca aatcgatact gaataaaacg gctaaaagac gaaccattat gaaaccatca
     6181 tcttcaaact cacgcagcaa aggtcatgca aaagcgcgtc gaaaaacacg cgaggagctg
     6241 gatcaggaag ctcgtgatcg caagcgtcag aaaaaacgtc gtggtcatgc gccgggcagc
     6301 cgcgcagcgg gcggaaacac cacgtcaggc agcaaaggcc agaacgcacc aaaagatcca
     6361 cgtattggca gtaaaacccc tattccattg ggcgtgactg aaaaagtcac caaacagcac
     6421 aaaccgaaga gtgagaaacc tatgctttca ccgcaggcgg agttggaatt actggaaacg
     6481 gatgagcgtc tggatgcgct gctggaacgt ctggaagcag gcgaaaccct gagtgccgaa
     6541 gagcaatcct gggtggatgc taaactggat cgtattgatg agttgatgca gaaactcggc
     6601 ctctcttatg acgatgacga agaagaggaa gaagacgaga agcaagaaga catgatgcgt
     6661 ctgctgcggg gcaactaacg gattgccgcc gtgggccttc ccgttctgct gataaccctt
     6721 ccggttatat gttatctggt gtggttattc gttaaactac agcggttgtc gcggcgacaa
     6781 aagtggctgc gcaaccggct gatgacccga aacgggcatc cgccggtacg ccgtagccgc
     6841 cagagacgcc atcggaagga gtgagcatgt ctgtacagca aatcgactgg gatctggccc
     6901 tgatccagaa atataactat tccgggccac gatacacctc gtacccgacc gcgctggagt
     6961 tttcagaaga cttcggcgaa caggcgtttt tacaagccgt ggcgcgctat cctgagcgtc
     7021 cattatctct ctacgtacat atcccgttct gccataagct ttgttacttc tgcggttgca
     7081 ataagattgt tactcgccag cagcacaagg ccgatcagta tctggacgcg ctggagcaag
     7141 aaatcgtcca tcgtgcaccg ctgttcgccg ggcgtcacgt cagccaattg cactggggcg
     7201 gcggaacgcc gacgtatctg aataaagcgc aaatcagccg cctgatgaag ctgctgcgcg
     7261 aaaacttcca gttcaatgcc gatgcggaga tttcgatcga agtcgatccg cgggaaatcg
     7321 aactggatgt actcgatcat ttacgcgccg aaggctttaa tcgcctgagc atgggcgtgc
     7381 aggacttcaa caaagaagtg caacgtctgg ttaaccgcga gcaggatgaa gagttcatct
     7441 ttgcactgct taaccatgcg cgtgagattg gttttacctc caccaacatc gacctgattt
     7501 acggcctgcc gaaacagacg ccggagagtt tcgcctttac cctgaaacgt gtggcggagc
     7561 tgaaccccga tcgtctgagt gtctttaact acgcgcatct gccgaccatt tttgctgctc
     7621 agcgcaaaat caaagatgct gacctgccga gtccgcagca aaaactcgat atcctgcagg
     7681 aaaccatcgc cttcctgacg caatcgggct atcagtttat cggtatggat cactttgccc
     7741 gtccggatga cgagctggcg gtggcccagc gtgaaggcgt gctgcatcgt aacttccagg
     7801 gctacaccac tcagggcgat accgatctgc tggggatggg cgtttccgcc atcagcatga
     7861 ttggcgactg ctacgcgcag aaccagaaag agttgaagca gtactatcag caagtggatg
     7921 aacaaggcaa tgcgctgtgg cgtggtattg cgctaacgcg tgatgactgt attcgccgcg
     7981 atgtgattaa gtcgctcatc tgcaacttcc gtctggatta cgcccctatt gagaaacagt
     8041 gggatttgca cttcgctgat tactttgcgg aagatctcaa gctgctcgcc ccgttagcaa
     8101 aagatgggct ggtggatgtg gatgagaagg gaatacaggt gacggcgaaa ggtcgcttgc
     8161 tgatccgcaa catttgcatg tgctttgata cctatctgcg ccagaaagcg cggatgcagc
     8221 agttctcacg ggtgatttga atagtgaatg gcgcttcgtt tacaaagtag aaaacgaaag
     8281 cgccatcaat gtgctgagaa caagattgcc tgatgcgcta cgcttatcag gcctacaaaa
     8341 cctgttgaaa atattgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
     8401 caaagagcac ttcatcagca atctgaaact caactaaaca gcccaatcat tgcggcacac
     8461 agcacggcag caacggcagt ttgcggtgtg tggctgtcaa ctgcgccact cgataccaga
     8521 ttaattattg attccagcat aatgactctc cccgttttcc gggcagatca tactgaactt
     8581 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8641 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     8701 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgcaac gcggtcgtca
     8761 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     8821 gcgctctgtc tgcccactgc gcaagaagcg tcgcccagct gtccggttgc atttgcgaag
     8881 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     8941 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9001 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9061 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9121 gttcgcgcag cggcggcaga tgaacgcgga taacgtttag gcggtggaac agatcctcac
     9181 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatccgca
     9241 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9301 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9361 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9421 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9481 cgataaacgg tgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9541 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9601 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9661 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
     9721 gcgccacggc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
     9781 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
     9841 gatggcgctg tttaatcttc ttgagcagcg ccagcccgtc cattcccggc atacggatat
     9901 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
     9961 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10021 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10081 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10141 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10201 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10261 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10321 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10381 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10441 gctgggatcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10501 taccacgcgt tcagccactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10561 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10621 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10681 accgccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    10741 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    10801 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    10861 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    10921 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    10981 caatttgcgg gagctttggg cgagcagttg ttgcgcggca gggttggcgt aatggatctc
    11041 caggttgtca tcgattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11101 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11161 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11221 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11281 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11341 cgcacgcggt catcttcttc gcgacgcaga gcgatgtacg catcaattgc ttcgtcagtg
    11401 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11461 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11521 ttgtccatgg cttcacccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11581 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11641 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    11701 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    11761 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    11821 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    11881 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    11941 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12001 tcgtcagctt ttttggtcat ggtattgaag cgggtagcca cttcgttctg accagcagtc
    12061 gctacttcgt ggtgatgggc ttcaaccacc agccccatct gttccatcac cagacacatt
    12121 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12181 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12241 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12301 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12361 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12421 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12481 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12541 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12601 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12661 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    12721 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    12781 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    12841 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    12901 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    12961 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13021 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13081 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13141 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13201 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13261 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13321 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13381 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13441 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13501 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13561 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13621 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13681 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    13741 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    13801 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    13861 gtatcgaaac cgacctggcg gaagctggcg atatcgttgc gatcacgggc cttggcgaac
    13921 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    13981 ttgatgagcc gactgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14041 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14101 acgttgcgct gcgcgtagaa gaaaccgaag acgctgatgc gttccgcgtt tctggtcgtg
    14161 gcgaactaca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14221 tatcccgtcc gaaagttatc ttccgtgaaa tcgacggtcg taaacaagag ccatatgaaa
    14281 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14341 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14401 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14461 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14521 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcgctgt
    14581 tcggtctgca ggatcgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14641 agattatcgg tattcatagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    14701 aactgaccaa catgcgtgct tccggtactg acgaagccgt tgttctggtt ccgcctatcc
    14761 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    14821 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    14881 gcgcaccgaa agactattaa tttcgttttt tagttataaa aaacctgcca tcgctg
//
LOCUS       DAJTOR010000094        14742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTOR010000094 DAJTOR010000000
VERSION     DAJTOR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.094"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            517..1434
                     /locus_tag="OO249_002556"
     CDS             517..1434
                     /locus_tag="OO249_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636814.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            1519..2391
                     /locus_tag="OO249_002557"
     CDS             1519..2391
                     /locus_tag="OO249_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5636815.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            2763..5609
                     /gene="ag43"
                     /locus_tag="OO249_002558"
     CDS             2763..5609
                     /gene="ag43"
                     /locus_tag="OO249_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5636816.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            5680..5838
                     /locus_tag="OO249_002559"
     CDS             5680..5838
                     /locus_tag="OO249_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5636817.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            5993..6811
                     /locus_tag="OO249_002560"
     CDS             5993..6811
                     /locus_tag="OO249_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5636818.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            7153..7626
                     /locus_tag="OO249_002561"
     CDS             7153..7626
                     /locus_tag="OO249_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5636819.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            7642..8118
                     /locus_tag="OO249_002562"
     CDS             7642..8118
                     /locus_tag="OO249_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5636820.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            8181..8402
                     /locus_tag="OO249_002563"
     CDS             8181..8402
                     /locus_tag="OO249_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5636821.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            8565..8939
                     /locus_tag="OO249_002564"
     CDS             8565..8939
                     /locus_tag="OO249_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5636822.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            8986..9360
                     /locus_tag="OO249_002565"
     CDS             8986..9360
                     /locus_tag="OO249_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5636823.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            9357..9848
                     /locus_tag="OO249_002566"
     CDS             9357..9848
                     /locus_tag="OO249_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636824.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            9860..10057
                     /locus_tag="OO249_002567"
     CDS             9860..10057
                     /locus_tag="OO249_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636825.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            10142..10702
                     /locus_tag="OO249_002568"
                     /pseudo
     CDS             10142..10702
                     /locus_tag="OO249_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            10774..11778
                     /locus_tag="OO249_002569"
     CDS             10774..11778
                     /locus_tag="OO249_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5636826.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            12060..12341
                     /locus_tag="OO249_002570"
                     /pseudo
     CDS             12060..12341
                     /locus_tag="OO249_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(12491..12578)
                     /locus_tag="OO249_002571"
     tRNA            complement(12491..12578)
                     /locus_tag="OO249_002571"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12542..12544),aa:Ser,seq:gga)
     gene            12812..13750
                     /gene="ghrA"
                     /locus_tag="OO249_002572"
     CDS             12812..13750
                     /gene="ghrA"
                     /locus_tag="OO249_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR5636827.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            13805..14542
                     /gene="ycdX"
                     /locus_tag="OO249_002573"
     CDS             13805..14542
                     /gene="ycdX"
                     /locus_tag="OO249_002573"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5636828.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            14566..14736
                     /gene="ycdY"
                     /locus_tag="OO249_002574"
                     /pseudo
     CDS             14566..14736
                     /gene="ycdY"
                     /locus_tag="OO249_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
BASE COUNT         3676 a         3740 c         3827 g         3499 t
ORIGIN      
        1 ggcataaagg aggtaccttt ctggcgttta taactctctt tatcggtgac gccggtaata
       61 cagggaatac cacaccgaca aacgaggtat taacagacga tatttcagtg tccataattt
      121 ttctgtccgg ttaaataacg tcagtctgct gccctctgac tgtaatcatc aaagccggaa
      181 tgcataccgg gttacaacgt aaaaaaagtg atgctggccc ccggtgcagc tggcgggcga
      241 aacatgttta cctttcaata ccccctgtct gcagtctcca ctcttccatc ctcttaagct
      301 acgtcggcca catgagtgca cttccatgat aacaggtatt cctgtcagca taacgaagcc
      361 ggttaccttc ccttcctcct gcatttcctg tcagaatgac tatacagcgc gcagattgcc
      421 tttttattga ccatcttaat ataccgttat aaacttcttt ttatcatatt attatcacct
      481 tattatgttt aatttttaag cagtcggaga aagaggatgg ttcagaaaat tttgtcggat
      541 aaggttatga atgaaagaac aaatgcgtat tactcttatt atcttggaga gcgtaatatc
      601 tccgtactgc cccttaatgt ttatgatccc ccggaacgtt ttatcgctta cataaaaaaa
      661 aacagagaaa atctgaacat cacactttca gattttgaac ttgaacaaat aatttctggt
      721 atgcgtctga aggccctggc cttcctggtc cccctggaaa aaatatcctg gatcgcgggc
      781 agtgaacgcg catgcttatt ttcctggtat ctgttgatgc agtttatcca gaataacagg
      841 gcaaaaataa gcgcagactt gcttcaaaag aataaactgt atcttaaaga agagtatctg
      901 gaagggaatg ccttcccgtc ggactcatcg acacagttca gacaaatact cagagttctc
      961 gatattcttt ctgataagaa tttgcgggat gagtggataa tccagaccaa agacagatgg
     1021 atgcgggcat ttaaatcaaa gagtcctttc agttacctcc tcccggagaa cgaacatgaa
     1081 tgcatatgga catggaatta tttgaaggga aaaaatattg cactggaaaa actggccagt
     1141 tttcctggtt ctgctgatat ttaccacgca attcaccttt catttgacat atgggttacc
     1201 tgtccactca cctcacctga cgatataaaa aatttcagaa acagtttcaa taaggccaag
     1261 gcgcagcgca agtacaaaaa aatgcaggaa gataaagtca atgtacagtt ttttcttgac
     1321 gcagaaacca gggcgcagct caaagaatta tcgagggtca gaagactcag taccggcgaa
     1381 atgctgcatg acctgattgt tgaagagtat aaaaggtacc gacattccag ataatttcag
     1441 acatatatta tctccgtgag tcccccgcca cctttccggt gtgcgggggt tttgtctttt
     1501 ttcaccggga atacatgtat gaatccgtct gatgccattg aggcaattga aaaaccgctc
     1561 tcctccctgc cttactcact ttcccgtcac atcctggaac atctgcgcaa actcaccagt
     1621 cacgaacccg tgattggcat tatgggtaaa agcggggccg gtaaatcctc actctgtaat
     1681 gcactgtttc agggggaggt caccccggtc agtgatgttc acgccggcac ccgggaagtg
     1741 cagcgtttcc gtctgagtgg ccatggtcac agcatggtta tcactgacct gcccggggtg
     1801 ggcgagagcc gggacagaga tgcagagtat gaagccctgt accgtgacat tctgcctgaa
     1861 cttgacctgg tactgtggct gattaaagcc gatgaccgtg ccctgtctgt ggatgagtat
     1921 ttctggcgac acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc
     1981 gacaaaacgg agccctgcca tgaatgggat atggccggta ttcagccttc tcccgcacag
     2041 gcacagaata ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg
     2101 gttgttgccg tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctaatg
     2161 acagcacttc ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc
     2221 acggaatctg tccggggtca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt
     2281 gacacggcgg agagcgtctg tgttgcctct gttgcccgta cggttctgcg tgccgtccgt
     2341 gacacggtgg tctctgttgc ccgcgctgta tggaactgga ttttcttctg aacagtctgc
     2401 cagggaaatg cctgataaca ttacgttaac caactgaaaa catgacacaa acccctcata
     2461 ctcctcctgt ttctcttgcg tgaccgcctt gtcgttaata gaataaaact atcgataaaa
     2521 cagatatcga tcgttgctat cgatcgttta tagtggttaa tggtctgcga aaaggcagct
     2581 gaatctcttc atcatgcaga acggaattgc acacaacgga ctgatacttc tctggcggat
     2641 gagagagggg aagcatttat gtccgggaaa aaccacttta tctgaccatg ctgttttgta
     2701 cctgccggta tccacttttg tgggtaccgg cttttttatt caccctctgt aaggaaaagc
     2761 tgatgaaacg acatctgaac accagctaca ggctggtatg gaatcacatt acgggcaccc
     2821 tggtggtggc ttccgaactg gcccgctcac ggggaaaacg cgccggtgtg gcggttgtgc
     2881 tgtctcttgc tgctgtcaca tcagtcccgg cactggctgc tgacacgatt gtacaggcgg
     2941 gagaaaccgt gaacggcgga acactgacaa atcatgacaa ccagattgtt ctcggtacgg
     3001 ccaacggaat gaccatcagt agcggtctgg agtatgggcc ggataacgag gccaataccg
     3061 gcgggcaatg gatacaaaat ggcggtatcg ccaacaacac tactgtcacc ggtggtggtc
     3121 ttcagagagt gaatgccgga ggaagcgttt cagacacggt tatcagtgcc ggaggcggac
     3181 agagccttca ggggcaggca gtgaatacca ctctgaacgg cggtgagcag tgggtacatg
     3241 aaggcgggat tgcaacgggt accgtcatta atgagaaggg ctggcagacc gtcaaatccg
     3301 gcgcaatggc aaccgacacg gttgtgaata ccggcgcgga agggggacca gatgcagaaa
     3361 atggtgatac cgggcagttt gttcgcggaa atgccgtacg taccactatc aatgaaaatg
     3421 gtcgtcagat tgtggctgct gaaggaacag caaataccac tgtggtttat gccggcggcg
     3481 accagacggt acacgggcat gcgctggata ccacactgaa tggcggttac cagtatgtgc
     3541 acaacggcgg tacggcgtct ggcactgttg taaacagtga cggctggcag attatcaagg
     3601 aaggtggtct ggcggatttc accaccgtta accagaaagg caaactgcag gtgaacgccg
     3661 gtggtaccgc cacacatgtc accctgaaac agggtggagc actggttacc agtactgcgg
     3721 caacggttct tggcagcaac cgtctgggca atttcacggt ggaaaacggt aaggctgacg
     3781 gagttgttct ggagtccggc ggtcgcctgg atgtactgga aggccattca gcgcagaaaa
     3841 cacgggtgga tgacggcgga accctggcag tgtctgccgg tggtaaggca acaggtgtca
     3901 ccatgacatc cggtggtgcc ctgattgcag acagtggtgc cactgttgag gggaccaatg
     3961 ccagcggtaa gttcagtatt gatggcacat ccggtcaggc cagcggactg ctgctggaaa
     4021 atggcggcag ctttacggtt aatgccggag gactggccag caacaccact gtcggacatc
     4081 gtggaacact gacgctggct gccgggggaa gtctgagtgg cagaacacag ctcagtaaag
     4141 gtgccagcat ggtactgaat ggcgatgtgg tcagtaccgg cgatattgtt aacgcagggg
     4201 agattcgctt tgataatcag acgacaccgg atgccgcgct gagccgtgct gttgcaaaag
     4261 gcgactcccc ggtaacgttc cataaactga ccaccagtaa cctcaccggt cagggtggca
     4321 ccatcaatat gcgtgttcgc cttgatggca gcaatacctc tgaccagctg gtgattaatg
     4381 gtggtcaggc aaccggcaaa acctggcttg cgtttacaaa tgtcggaaac agtaacctcg
     4441 gggtggcaac ctccggacag ggtatccggg ttgtggatgc acagaatggc gccaccacag
     4501 aagaaggtgc gtttgccctg agtcgcccgc ttcaggccgg cgcctttaac tacaccctga
     4561 accgtgacag cgatgaagac tggtacctgc gcagtgaaaa tgcttatcgt gctgaagtcc
     4621 ccctgtatac atccatgctg acacaggcaa tggactatga ccggattctg gcaggctccc
     4681 gcagccatca gaccggtgta aacggtgaaa ataacagcgt ccgtctcagc attcagggcg
     4741 gtcatctcgg tcacgataac aacggcggta ttgcccgtgg agccacgccg gaaagcagcg
     4801 gcagctatgg cttcgtccgt ctggagggtg acctgctgag aacagaggtt gccggtatgt
     4861 ctctgacgac aggggtgtat ggtgccgcag gccattcttc cgttgatgtt aaggatgatg
     4921 acggttcccg cgccggcacg gtccgggatg atgccggcag cctgggcgga tacctgaatc
     4981 tgacacacac gtcctccggc ctgtgggctg acattgtggc acagggaacc cgccacagca
     5041 tgaaagcgtc atcggacaat aacgacttcc gtgcccgggg ctggggctgg ctgggctcac
     5101 tggaaaccgg tctgcccttc agtatcactg acaacctgat gctggagcca caactgcact
     5161 atacctggca gggacttacc ctggatgacg gccaggataa cgccggttat gtgaagttcg
     5221 ggcatggcag tgcacaacat gtgcgtgccg gtttccgtct gggcagccac aacgatatga
     5281 gctttggtga aggcacctca tcccgtgaca ctctgcgcga cagtacaaaa caccgtgtga
     5341 gtgaactgcc ggtgaactgg tgggtacagc cttctgttat ccgcactttc agctcccggg
     5401 gtgacatgag catggggaca gccgccgccg gcagtaacat gacgttctca ccgtcccgga
     5461 atggcacgtc actggacctg caggccggac tggaagcccg tgtccgggaa aatatcaccc
     5521 tgggcgttca ggccggttat gcccacagcg tcagcggcag cagcgctgaa ggttataacg
     5581 gtcaggccac gctgaatgtg actttttgat aatcaggtca ctctccttca ggccccggtc
     5641 atcatgaccg gggcttttta ctttcaggga gcaacagata tgccgggttg tacatcacgt
     5701 ttattgcccg aaggcccttt ctcacgcgaa caggctgttg ccgtcaaaac agcttaccgc
     5761 aatgtgttta ctgaagatga ccagggaaca tattcccggc tggttatccg taatgctgaa
     5821 ggtcagttgc gctggtgatg ccggaacaca gcttaatact cagctcgcca gttaagggct
     5881 tctcagacaa taaacgtcat tcatttcatc tgtcaggccg cgtcttctcc gggagacgca
     5941 gctttttcat ttattacacc agtaaatcgt aaccaccgat aaggagcaaa ttatgcgact
     6001 agccagtcgt tttggttatg cgaaccagat acgccgtgac cgtccgctga cacacgaaga
     6061 actgatgcac tatgttccca gtattttcgg tgaagaccgg catacttccc gcagtaaacg
     6121 atatgcgtac attcccacca tcaccgtact ggaaagcctg cagcaggaag gttttcagcc
     6181 attcttcgcc tgccagaccc gcgtgcgcga cccgggccgc cggggataca caaaacacat
     6241 gctgcgtctg cggcgggccg gagagataaa cggagaacat gtccctgaaa ttattctgct
     6301 caactctcat gacggtacct ccagctacca gatgctgccg ggttacttca gattcgtctg
     6361 ccagaatggc tgcgtctgcg gtcagtctct gggggaagtg cgtgttccac accggggaaa
     6421 tgtggtggag aaagttatcg aaggggctta cgaagtggtg ggggtttttg accggattga
     6481 ggaaaagcgt gatgccatgc agtcgctggt cctgccgcca ccggcacgcc aggcgctggc
     6541 acaggcggca ctgacttacc gttatggtga cgaacatcag ccagtcacca ccgctgacat
     6601 tctgacacca cgacgcaggg aggattacgg taaagacctg tggagcgcat atcagaccat
     6661 ccaggagaat atgctgaaag gcgggatttc cggccgcagt gcaaaaggaa aacgtatcca
     6721 cacccgtgcc attcacaaca tcgacaccga tattaagctc aaccgcgcat tgtgggtaat
     6781 ggcagaaacg ctgctggaga gcctgcgctg atgcagcatg tccctgaagg cgtgattcag
     6841 gcaccagaat cacccgacgc cccggaggcg ctgccggggt gtcgttccat ttctgatgat
     6901 gacctgtgcg cctggtgtac ccgtctgctt taccgtcccg gagaaatcag tctgtgcaga
     6961 ctgagtatac aggcaatggc atctggccat ccgtctgtga ccggaacggt tacgcgtacg
     7021 gctgtccgga atttcagccg aatatcattc atccctgata ttaccgggtc atcacggtca
     7081 gtgatgaacg tgatgttgcc agctcccgac ctttactccc tctcatttat tcacattaca
     7141 aggatttcat acatgaaaac attgtctcag aacaccaccg cttcggcctg tgcaccggag
     7201 actggcctgc aacaactggt tgccactccc gtccctgatg aacagcgcat cagcttctgg
     7261 ccgcagcatt ttggcctcat tccacagtgg gtcaccctgg aaccccgtgt cttcggctgg
     7321 atggaccgtc tgtgtgaaga ctactgcggt ggtatctgga atctgtacac cctgaacaac
     7381 ggcggggcat ttatggcacc cgaaccggat gacgatgatg acgaaacatg ggtactgttc
     7441 aatgccatga acggtaaccg cgctgaaatg agcccggaag ccgccggcat tgctgcctgt
     7501 ctgatgacgt acagtcatca tgcctgtcgt acggagtgtt atgccatgac ggtccattat
     7561 taccggctgc gggattacgc cctgcagcat ccggaatgca gcgccattat gcgcatcatc
     7621 gactgaatgg aggcagggac aatgcagcag ctttcctttc tgcccggaaa catgacgccc
     7681 ggcgagcgca gtctcattct gcgggccctg caaaccctgg accgccatct tcatgaaccc
     7741 ggcgtggcct tcacctccac ccgtgcggca cgggaatggc tgattctgaa catggcggga
     7801 ctggagcggg aagagttccg ggtgctgtat ctgaacaacc agaatcagct gattgccggt
     7861 gaaaccctct tcaccggcac catcaaccgt acggaagttc atccccggga agtgattaaa
     7921 cgcgccctgt accacaatgc cgctgccgtg gtgctggcgc acaatcaccc gtccggtgaa
     7981 gtcacaccca gtaaggcaga ccggcttatc accgaacgtc tggtacaggc actgggcctg
     8041 gtggatatcc gggtgccgga ccatctgata gtcggtggca accaggtttt ctcctttgcc
     8101 gaacatggtc tgctttaacc cgtcacaacc acatcacacc tgttttcact tttatcttct
     8161 gtcttcagag gtatcacatc atgagaatca tcacccgtgg tgaagccatg cgtattcacc
     8221 aacagcatcc tgcatcccgt ctttttccgt tctgtaccgg taagtatcgc tggcacggca
     8281 gtgctgaagc gtatactggt cgtgaagtgc aggatattcc cggcgttctt gccgtgtttg
     8341 cagaacgccg taaggacagt tttggcccgt atgtccggct gatgagtgtc accctgaact
     8401 gatgttccgt cacccgggaa acgagtacga ccagatgacc tcagacgccg ctgtggatga
     8461 ggccattctc atcaatgcat acatcttcac cgaaaccggt cagcgcgccg gctgacctgt
     8521 catcgttaaa ccattcttaa ccacttttca gagaggattt tatcgtgtca gacaaactct
     8581 ccgggataac tcatcccgat gacaatcacg accgcccctg gtgggggctt ccctgcacag
     8641 tgaggccctg ttttggcgct cgtctggtgc aagagggtaa ccgcctgtat taccttgccg
     8701 accgcgccgg tatcagaggc cgcttcagcg acgcagatgc ataccatctg gaccaggcct
     8761 ttccgctgct gatgaaacaa ctggaactca tgctcaccag cggtgaactg aatccccgat
     8821 atcagcatac cgtcacgctg tatgcaaaag ggctgacctg cgaagccgac accctcggct
     8881 cctgtggtta cgtttatctg gctgtttatc cgacaccagc acccgcaacc acctcataac
     8941 cccgaagctc tcacctttag cactaccagt tacagagaac ttaagatgaa aacattatcc
     9001 gacacacatg tacgggaggt atctcgctgc ccgtctcccg tcaccatctg gcagacactg
     9061 ctcatccgac tgctggacca gcattatggc ctcacactga atgacacacc gtttgtcgat
     9121 gaacgtgtga ttgagcagca tattgaggca ggcatttcac tgtgtgatgc agttaatttt
     9181 ctcgtggaaa aatacgcgct ggtgcgtacc gaccagccgg gattcagcac ctgtccccgc
     9241 tctcagttaa taaacagtat tgatatcctc cgggctcgcc gggcgaccgg cctgatgacc
     9301 cgcgacaatt acagaacggt aaataacatt accctgggta agtatccgga ggcgaaatga
     9361 tgaaactggc cctgacactg gaagccgaca gcgttaacgt acaggcactg aacatagggc
     9421 gcattgtcgt tgacgtcgat ggtattgagc tcgctgaact gattaacatg gtctgcgata
     9481 acggctactc ccttcgtgtt gttgatgaat ctgaccagac ctcagcagaa tgcacgccac
     9541 catttgctac ccttaccggc atacgctgca gtaccgcaca tatcacggaa acggacaacg
     9601 cctggttgta ctcgctgtca caccagacca gtgacttcgg tgaatcagaa tggattcatt
     9661 ttacaggtag cggatatctg ttacgtaccg atgcgtggtc atacccggtt ctgcggctta
     9721 aacgcctggg gctgtcaaaa acgttccgtc gtctggtcgt cacactcatc cggcgttatg
     9781 gcgtcagtct cattcatctg gatgccagcg ctgaatgcct gccgggttta cccactttcg
     9841 actggtaacc aggaacaaca tgaaatcatt aaccacggaa acagcgctgg atattctgat
     9901 tgcgtggctg caggacaata tcgactgcgg atcgggaatt atctttgaca acgatgagga
     9961 taaaacggat tcagcagcac tgttgccctg tatcgaacag gcccgggagg atgtccgtac
    10021 cctgcgtcat ctgcagcttc tgcaccagaa ccggtgaagc tcactcacca gacttcattc
    10081 ctctgacgcc agcctgcaca gggctggcgt tttcatttat ctgcaaaaag gaatatcgat
    10141 tatgtcagat atcacaatct cccgtccgga agtggtcaac gggcatacgg acgttatctg
    10201 ctcaaccagc attcgccaca ttctggctgt acgaaagagt acactgttgc aaatcgacac
    10261 acttatccga caactggctg aaatctcggt gctgacggaa agcatcggag gtaaaactgc
    10321 cccggactgg gccatgaaac aggattttcg ctgcggctgc tggctgatgg agaaacccga
    10381 aacagcaatg aaagccatta cccggaatct tgatcgcgaa atctggcgtg acctgatgca
    10441 acgttccggg atgctgtcat taatggatac gcaggcccgt gatacatggt accggtcact
    10501 ggagtacgat aattttccgg aaatcagcga agcgaacatt ctgagcacat ttgaacaact
    10561 gcaccagaat aaggatgagg tgtttgagcg aggggtgatc aacgtcttca gagggctgaa
    10621 ctggaattac aaaaccaatt gcccctgtaa atttggcagt aaaattatcg tcaacaatct
    10681 ggtgagatgg gaccggtggg gataatgata tggtcacccc caccttaaca gcagtacgct
    10741 gttaaggtaa atccctttaa cggagagacc atcatgcaga acatcacact tattggtatc
    10801 gatctcggaa agcactcctt ccacattcat tgtcaagaca agtctggtaa ggctcttctg
    10861 cgtaaaaaat ttacccgtac taaactgatg cagtttctgg cgacatgtcc gccctcagtt
    10921 gttgtaatgg aagcctgtgc gggtgcacac tttatggctc gctgtatctc tgattttggg
    10981 catgaaacta agctgatttc tccacagttc gttcgcccgt ttgtcaaaag taataagaat
    11041 gattttgttg atgcagaagc catatgcgaa gcggcttcac gaccatcaat gcgttttgtc
    11101 cagccaagaa ctgagaccca gcaggccatg agggcgttac accgggtcag agaatcgctc
    11161 atcagagata aggtgaaaac aaccaaccag atccacggtt tcttgctgga atttggaatc
    11221 agcctgccga taggtgatgc tgtaataaaa cggttgtctc tcgtgttggc tgaacacgaa
    11281 atccccgaat atctgagccg ccttttggtg aggttgcata cccactactt ttaccttgtt
    11341 gagcaaatag cagagctgga atcagaatta agtcagtcaa tcaacgctga tgataccgct
    11401 cagcgcatta tgacaattcc tggtgttggt ccgattactg ccagcttact atcgtcgcaa
    11461 cttggtgacg ggaaacagtt ctcatgcagc agggattttg cagcttccac aggacttgtt
    11521 ccccggcagt atagcactgg aggaaaatcg actctgttgg gtatcagtaa gcgaggtgat
    11581 aaaaatttac gccggttact ggttcagtgt gcccgctcat tcataatgcg acttgaacgc
    11641 aagcatggaa aacttgctga gtgggttcgg gagcaactca acaagaagca ttcgaatgtg
    11701 gttacctgcg ctctggcaaa taaactggct cgaattgcct gggcaatcac tactcagaaa
    11761 aaagaatatc aggcctgata tagatgctga tataactcaa ttagtttatt ttaaacaata
    11821 agatacaagt tacctttctg gttttgcgaa gactgattat tgatgacatg aacggcaaat
    11881 cgacctgatt aaaaacctaa cagatgaaat ggctcactga agccgagggg atttttagga
    11941 ttatcaggtg cggaactcat cggggcgcgg gcatccttgc ccacagagac gccggataga
    12001 tttaagcaag ccaaccatta atcaaaaatc agtgttgcaa aaacgggggt gaccatagat
    12061 ttcatcttat caccggacaa caggctgacc gactcgccga tctggaaaga atgttgcacc
    12121 tgttcagcgg taaaccaatc cccgacaacc ggggaaacat cacccttcgt ctggatgatc
    12181 acattcagtc tgttcagggt aaagagagct atgaagatga gatgttcagc atcagatact
    12241 ttaagaaggg ctccgcgcat atcacgttca gaaaaccaga actggttgac agactgaatg
    12301 atattatcgc aaaacactat cctgatatgc tggcagtata gctttgccag tatgctcaaa
    12361 gatcggaaat tgtccgataa gatatgatcg cggggcatat aaaccggttt cagatggcat
    12421 acccgatggc atactgtctg gaaggaaaaa taaaaaagcg catgttaaac aataacatgc
    12481 acttataatc tggcggtgag ggggggattc gaacccccga tacgttgccg tatacacact
    12541 ttccaggcgt gctccttcaa ccgctcggac acctcaccaa attgttgttc ctgtcttgct
    12601 ggaacgggcg ctaatttagg gaaatcatga cctgaggtca acaaactttt tgaaaaaatc
    12661 gcgcgtttat tcaaacttca atcaatgtgt ggttttaata agcgaaatct gctttttttg
    12721 ccaccgacca cggatttgtt atgctggtgg cctttgtaga tcataacgat aagtgcgaat
    12781 aaatttcgca caacgctttt cgggagtcag tatggatatc atcttttatc acccaacgtt
    12841 cgatacccaa tggtggattg aggcactgcg caaagctatt cctcaggcaa gagtcagagc
    12901 atggaaaagc ggagataatg actctgctga ttatgcttta gtctggcatc ctcctgttga
    12961 aatgctggca gggcgcgatc ttaaagcggt gttcgcactc ggggccggtg ttgattctat
    13021 tttgagcaag ctacaggcac accctgaaat gctgaaccct tctgttccac tttttcgcct
    13081 ggaagatact ggtatgggcg agcaaatgca ggaatatgct gtcagtcagg tgctgcattg
    13141 gtttcgacgt tttgacgatt atcgcatcca gcaaaatagt tcgcattggc aaccgctgcc
    13201 tgaatatcat tgggaagatt ttaccatcgg cattttgggc gcaggcgtac tgggcagtaa
    13261 agttgctcag agtctgcaaa cctggcgctt tccgctgcgt tgctggagtc gaacccgtaa
    13321 atcgtggcct ggcgtgcaaa gctttgccgg acgggaagaa ctgtctgcat ttctgagcca
    13381 atgtcgggta ttgattaatt tgttaccgaa tacccctgaa accgtcggca ttattaatca
    13441 acaattactc gaaaaattac cggatggcgc gtatctcctc aacctggcgc gtggtgttca
    13501 tgttgtggaa gatgacctgc tcgcggcgct ggatagcggc aaagttaaag gcgcaatgtt
    13561 ggatgttttt aatcgtgaac ccttaccgcc tgaaagtccg ctctggcaac atccacgcgt
    13621 gacgataaca ccacatgtcg ccgcgattac ccgtcccgct gaagctgtgg agtacatttc
    13681 tcgcaccatt gcccagctcg aaaaagggga gagggtctgc gggcaagtcg accgcgcacg
    13741 cggctactaa taaagcatca ggattcctgc tatccttggc gggaattgaa tacaggagag
    13801 agttatgtat cccgtcgacc ttcatatgca taccgttgcc agcacacatg catatagcac
    13861 attgagtgat tacattgccc aggccaaaca aaagggcatt aaactttttg cgatcaccga
    13921 tcatggcccg gatatggaag atgctccgca tcactggcac ttcattaaca tgcgtatctg
    13981 gccgcgagtg gttgatgggg tagggatcct gcgcggcatc gaagctaaca ttaaaaatgt
    14041 tgatggtgaa attgattgca gcggtaaaat gtttgactcg ctggatctga ttattgccgg
    14101 ttttcatgag ccggtttttg caccacatga caaagcgacc aatacacaag cgatgatcgc
    14161 cactatcgcc agcggcaatg tgcatataat aagccatccc ggaaatccca aatatgaaat
    14221 agatgtgaaa gccgttgctg aagcagccgc gaaacatcag gtggcgctgg aaatcaataa
    14281 ttcttcattt ttacactcac gtaagggcag tgaagacaac tgtcgtgcgg tagccgcagc
    14341 ggtacgtgat gctggaggtt gggtggcatt aggctcggat tctcacaccg cgtttaccat
    14401 gggggaattt gaagagtgtc ttaaaatcct cgacgcggta gattttccgc cagagcgcat
    14461 tttgaatgtt tctccgcgcc gcttactgaa cttccttgaa tctcgcggta tggcaccgat
    14521 tgcggaattt gcagaccttt aattactagt aatggaaata tattaatgaa cgagttttct
    14581 atcctctgtc gtgtgctggg ttcgctctat taccgccaac cgcaagatcc tttactggtg
    14641 ccgctgttta ccctgattcg tgaggggaaa ctggctgcga actggccact ggagcaggat
    14701 gagttactga cgcgtttaca gaaaagctgt gatatggcgt aa
//
LOCUS       DAJTOR010000095        14559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTOR010000095 DAJTOR010000000
VERSION     DAJTOR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.095"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO249_002575"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO249_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5636829.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO249_002576"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO249_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5636830.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO249_002577"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO249_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5636831.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO249_002578"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO249_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5636832.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO249_002579"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO249_002579"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5636833.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO249_002580"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO249_002580"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5636834.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO249_002581"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO249_002581"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5636835.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO249_002582"
     CDS             complement(4598..5320)
                     /locus_tag="OO249_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5636836.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO249_002583"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO249_002583"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5636837.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO249_002584"
     CDS             6520..6738
                     /locus_tag="OO249_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5636838.1"
                     /translation="MQDLSLEARLAELESRLAFQESTIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO249_002585"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO249_002585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5636839.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO249_002586"
     CDS             complement(7472..7672)
                     /locus_tag="OO249_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5636840.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OO249_002587"
     CDS             complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OO249_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR5636841.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEE
                     DEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYY
                     GDATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHE
                     LLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHA
                     DTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO249_002588"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO249_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5636842.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELANHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OO249_002589"
     CDS             10175..12088
                     /locus_tag="OO249_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5636843.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OO249_002590"
     CDS             12088..13110
                     /locus_tag="OO249_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5636844.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OO249_002591"
     CDS             13104..13322
                     /locus_tag="OO249_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5636845.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OO249_002592"
     CDS             13376..14245
                     /locus_tag="OO249_002592"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5636846.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..>14559)
                     /locus_tag="OO249_002593"
     CDS             complement(14300..>14559)
                     /locus_tag="OO249_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5636847.1"
                     /translation="IDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTG
                     RDLKDAAVARAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
BASE COUNT         3598 a         3968 c         3587 g         3406 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtctag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggtgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaagcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg
     6961 aatcgccaca acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggcc cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggataa ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ttcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ctgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccggcgt tatcgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc aaggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcaacaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcaag cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gcgacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtgatatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc cctcgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atattatgcc gcgcagtcag tttcgcgaat
    13321 aacctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga agcgaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg aacattcacc ccctggcaac ctttaccaga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtagtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctg gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcggc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atcgaaacca catcgatgg
//
LOCUS       DAJTOR010000096        14556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTOR010000096 DAJTOR010000000
VERSION     DAJTOR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.096"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..422
                     /locus_tag="OO249_002594"
     CDS             <1..422
                     /locus_tag="OO249_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5636848.1"
                     /translation="LMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRL
                     AEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTL
                     SIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            438..920
                     /locus_tag="OO249_002595"
     CDS             438..920
                     /locus_tag="OO249_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5636849.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(997..1344)
                     /gene="rplS"
                     /locus_tag="OO249_002596"
     CDS             complement(997..1344)
                     /gene="rplS"
                     /locus_tag="OO249_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5636850.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1386..2153)
                     /gene="trmD"
                     /locus_tag="OO249_002597"
     CDS             complement(1386..2153)
                     /gene="trmD"
                     /locus_tag="OO249_002597"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5636851.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2184..2732)
                     /gene="rimM"
                     /locus_tag="OO249_002598"
     CDS             complement(2184..2732)
                     /gene="rimM"
                     /locus_tag="OO249_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5636852.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEDYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(2751..2999)
                     /gene="rpsP"
                     /locus_tag="OO249_002599"
     CDS             complement(2751..2999)
                     /gene="rpsP"
                     /locus_tag="OO249_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5636853.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3136..4497)
                     /gene="ffh"
                     /locus_tag="OO249_002600"
     CDS             complement(3136..4497)
                     /gene="ffh"
                     /locus_tag="OO249_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5636854.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4664..5455
                     /locus_tag="OO249_002601"
     CDS             4664..5455
                     /locus_tag="OO249_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5636855.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            5476..6762
                     /locus_tag="OO249_002602"
     CDS             5476..6762
                     /locus_tag="OO249_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5636856.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(6817..7410)
                     /gene="grpE"
                     /locus_tag="OO249_002603"
     CDS             complement(6817..7410)
                     /gene="grpE"
                     /locus_tag="OO249_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5636857.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            7533..8411
                     /gene="nadK"
                     /locus_tag="OO249_002604"
     CDS             7533..8411
                     /gene="nadK"
                     /locus_tag="OO249_002604"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5636858.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCKKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8497..10158
                     /gene="recN"
                     /locus_tag="OO249_002605"
     CDS             8497..10158
                     /gene="recN"
                     /locus_tag="OO249_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5636859.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10307..10648
                     /gene="bamE"
                     /locus_tag="OO249_002606"
     CDS             10307..10648
                     /gene="bamE"
                     /locus_tag="OO249_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5636860.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(10710..11000)
                     /locus_tag="OO249_002607"
     CDS             complement(10710..11000)
                     /locus_tag="OO249_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5636861.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10990..11427)
                     /gene="ratA"
                     /locus_tag="OO249_002608"
     CDS             complement(10990..11427)
                     /gene="ratA"
                     /locus_tag="OO249_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5636862.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            11598..12080
                     /gene="smpB"
                     /locus_tag="OO249_002609"
     CDS             11598..12080
                     /gene="smpB"
                     /locus_tag="OO249_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5636863.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12295..12657
                     /gene="ssrA"
                     /locus_tag="OO249_002610"
     tmRNA           12295..12657
                     /gene="ssrA"
                     /locus_tag="OO249_002610"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12818..14556)
                     /locus_tag="OO249_002611"
                     /pseudo
     CDS             complement(12818..14556)
                     /locus_tag="OO249_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         3588 a         3909 c         3431 g         3628 t
ORIGIN      
        1 cgttgatgaa caattccgat gcccgaaaaa cgtcggcgtt actatttctt gatggcgata
       61 atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagggtc ttgattgaaa
      121 tcgcaaaacg gttagctgaa tttggcgggc tgcgacataa agcataccgc ctgggcggcg
      181 atgaattcgc tatggtgctc tatgatgtac agtcggaatc tgaagtgcaa cagatatgct
      241 cagcactgac acaaatcttt aatctcccgt ttgatcttca taatggccat cagaccacca
      301 tgacattaag cattggttac gcgatgacca ttgagcacgc ctctgcggaa aaattacaag
      361 agcttgccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag ctggtgagat
      421 aacaaggata tatcgatatg ataaagcacc tggtagcccc cctgattttc acctcactaa
      481 tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc gccgctatgc
      541 aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca gatgccattc
      601 tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc caaaccatgg
      661 cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac accgataact
      721 atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc gtggccgatg
      781 catgggctat tggtggacaa attccacgca gcaatctcac cacacagggt ttaggaaaaa
      841 aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc cgcgtcgcag
      901 tggtgattac aaccccttaa aacacgagca aacagacaaa aaaagggcca gccaattggc
      961 cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct ctttgatacg
     1021 agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa cagcaccacg
     1081 acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac gctcaacgcc
     1141 ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt tacgaatagc
     1201 gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa cccatacttt
     1261 cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct gctcttgttc
     1321 aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac tgatatctcg
     1381 ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt tttgaactcc
     1441 gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc aggtcttcta
     1501 agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc atggttgccc
     1561 gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt atagtgcggg
     1621 caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg tcccagtacc
     1681 cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc accaccactg
     1741 agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac gcgctcatct
     1801 ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccaa ttcgctgacg
     1861 cccgcttgat caagcttgcg tccctgtggt gacagataaa tcacctttgc gccttcaccc
     1921 gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat taacatcccc
     1981 ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt gaagtcgcga
     2041 ggactccagc tctggatgct cagcaggcca tttttaactg cccggccagt taccccgtaa
     2101 tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca catagcgccg
     2161 tcttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc gattgaacga
     2221 gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag acgttccttg
     2281 ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattaga tccggtttcc
     2341 atcatatcga cgactttacc gagatcgtag tcttcagtgg ttactacctg gcagcccatc
     2401 aggtctttcc agtagtagtc accctcttca agctgaggca actgcgatga atccacgaca
     2461 atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt cagcttgatg
     2521 atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca ctgacccgcc
     2581 ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt ggaagaaaac
     2641 actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac gatgggatca
     2701 acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga ttaagctgct
     2761 ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc ctggccaacc
     2821 cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct agcgattggg
     2881 ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct gtcagcgaca
     2941 acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat agttaccata
     3001 acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc cggtgtcata
     3061 ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc aatcagcagt taataactct
     3121 gtcgcataag gtcatttagc gaccaggaaa tcccggcggc atcatcccct tcatgcttct
     3181 catcatcttc gccattccgc ccttcttcat tttcttcatc atgcgctgca tgtcgtcgaa
     3241 ctgtttcaga agacggttaa cgtcctgcac ctgcataccg caaccggcag caatacggcg
     3301 tttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca tcgagttgat
     3361 gatggcttcc atacgcacca gcactttatc gtccatctgt gacttgacgt tatccgggat
     3421 ctgccccatg cccggcagct tgcccatcag gctggccatg ccgcccatat ttttcatctg
     3481 gcgcagctgc tcaagaaagt cgttgagatc gaagccgtca ccttttttca gcttgctggc
     3541 taatttctct gcctgcgcgc ggtcaacttt gctttcgata tcttcgatca gcgacagtac
     3601 gtcgcccatg ccgagaatac gcgacgcgat gcggtccgga tggaacggct ccagcgcctc
     3661 agttttctcg ccaacaccga ggaacttgat cggtttgcca gtgatgtgac gaatagagag
     3721 cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac actacgccgg taagcggtaa
     3781 cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccggtcatgg cgtcaaccac
     3841 aaacagggtt tcaaccgggt taatcgacgc atggacttgt ttgatctcgt ccatcatcgc
     3901 ttcgtcaacg tgcagacgac cagcggtatc caccagcagc acgtcgtaga atttcagttt
     3961 ggcttctttc agcgccgcgt taacgatatc taccggcttc tgaccaacat cagaagggaa
     4021 gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg ccgggcgata
     4081 aacgtcggca gaaaccacca gcactttctt cttgtgcttc tcgcgcagga acttaccgag
     4141 tttaccaacg ctggttgttt taccggcacc ttgcaggccc gccatcagca cgaccgcagg
     4201 cggttgcgcc gccaggttca gggtctggtt ctcttcgccc atcgccgcaa ccagttcgtt
     4261 acggacgatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt catgaccaac
     4321 cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcga catccgcctc
     4381 cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag ttaggcgtcc
     4441 acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat caaacattgt
     4501 ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca cctcatcagt attttgccgc
     4561 agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt cacctgacgc
     4621 tatactgctt ctctttctta ttgctcaaac tgttgacatc actatgcccg tttttgctct
     4681 gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc tgctgcaaaa
     4741 aaacggcggc tggcggcgca tggctattat ttctgcggtc attgcgctgg tctgccacgc
     4801 aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc tcagcctgct
     4861 gaacgttggt tcattggtca gtttgatgat ctgtacggta atgaccattg tggcttctcg
     4921 caatcgtggc tggttgctgc tacccattgt ctatgccttt gcgcttatca acctggcgct
     4981 ggcgaccttc atgcccaatg aatacatcac ccatctggaa gcaacgcctg ggatgctggt
     5041 gcacattggc ttatcgctct tttcctatgc cacgctaatt atcgccgccc tgtacgcgct
     5101 gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcattta accaggaaat
     5161 gccgccattg atgagtatcg agcgtaaaat gttccacatc acgcagattg gcgtggtgct
     5221 gctaacgctc acgctttgca ctggcctgtt ctacatgcac aacctattta gcatggaaaa
     5281 tatcgacaag gctgtgctct ctatcgtggc gtggtttgtc tatattgtgc tgctgtgggg
     5341 acattaccat gaaggatggc gtggacgccg cgtcgtctgg tttaacgttg cgggcgcggt
     5401 aattctgaca ctggcctact tcggcagccg aattgtccag cagttaatca gctaaaccca
     5461 gaaaaggagt ttcccctgga acacatttct actactacgt tgatcattat tctgatcatc
     5521 atggtggtca tttcagccta tttttccggg tccgaaaccg gaatgatgac cctcaaccgc
     5581 tatcgtctgc gacatatggc gaaacagggt aatcgctcgg ccaaacgcgt cgaaaaattg
     5641 ctgcgtaagc cagaccgcct gataagcctg gtgttaatcg gcaataacct ggtcaatatt
     5701 cttgcctccg cgctcggcac tattgttggg atgcgtttgt acggcgatgc gggcgtggca
     5761 attgcgactg gtgtgctgac ttttgtcgta ctggtatttg ctgaggtatt gccgaaaacc
     5821 attgccgcgc tgtacccgga aaaagtcgct tatccgagta gttttctgct ggctccgctg
     5881 caaattttga tgatgccgct ggtctggttg ctgaatgcta tcacccgtat gctgatgcgc
     5941 atgatgggta tcaaaaccga tatcgtggtt agcggctctt tgagcaaaga agagttgcgc
     6001 actatcgtgc acgaatcacg ctcacaaatt tcccgtcgca atcaggatat gctgctgtcg
     6061 gtgctcgatc tggaaaaaat gaccgttgat gacatcatgg tgccgcgcag tgaaattatc
     6121 ggtattgata tcaacgatga ctggaaatcg attctgcgcc aactctccca ctcacctcac
     6181 gggcgcatcg tgctctaccg tgattcgctg gacgacgcca tcagtatgct gcgagtacgt
     6241 gaagcctggc gattgatgtc ggagaaaaaa gagttcacca aagaaaccat gctgcgcgcc
     6301 gcggacgaga tctattttgt gccggaaggt acgccgctca gcacgcagtt ggtaaagttt
     6361 cagcgcaaca aaaagaaagt cggcctggtc gtcaacgagt atggagacat tcaggggctg
     6421 gtgacggttg aagatattct ggaagagatt gtcggcgatt tcactacgtc gatgtcgcca
     6481 acacttgccg aagaggtcac gccgcaaaac gacggttcgg tgattatcga tggcaccgcc
     6541 aacgtgcggg aaatcaacaa agcctttaac tggcatctac cggaagatga tgcccgcacg
     6601 gttaatggcg tcattcttga ggcactggaa gagatccctg tcgcaggcac ccgcgtgcgt
     6661 attggcgagt acgatataga tattctcgac gtacaggaca atatgattaa gcaggtaaaa
     6721 gtttttcctg tgaaaccgct gcgcgagagt gtggcggagt aacgaaaacg gccctgcatt
     6781 cgcatgcagg gccgtgaatt attacgaaaa cagaaattac gcttttgctt tcgctacagt
     6841 taccatcgcc gcacgaatcg tacgaccatt cagcgtataa cccttctgca taatgcccag
     6901 tacgttacct ggcgcaacgt catcagattc caccattgcg atagcctgat gcacattcgg
     6961 gtccagcggc acgttagttt cggcgatcac ttcaacacca aacttacgca caacatccag
     7021 catcgacttc agcgtcagct caatgccttc aaccatcgca gacatatccg ggttagcttt
     7081 atcagccact tccagcgcac gatccaggct atcaatcacc ggcagcaatt cgttgatgaa
     7141 tttctccagc gcaaatttgt gggctttttc aatatccagt tcagtacgtc gacgcaggtt
     7201 ttccatttcg gcttttacac gcaaaatgcc gtcacgttca cgggtctggg cttcagccag
     7261 ctgagcttcg agattcgcaa ttttttcatc gcgcggatcc acctgctcag cagaagcttc
     7321 tggctcaact gcctcaatct cttcgtgctg atccatgata atttcttccg gggcttgccc
     7381 ctcaggcgtt ttctgttctt tactactcat gaatttctcc gcgttttttt cgcattcatc
     7441 tcgctaactt cgcttattat ggggatcagt ttcagggttt caagggaagc actcacattg
     7501 tcatcaatct tcgcaacaag gacctcggaa aaatgaataa tcatttcaag tgtattggca
     7561 ttgtgggaca cccacggcac cccactgcac tgacaacaca tgaaatgctc taccgctggc
     7621 tgtgcaaaaa aggttacgag gtcatcgttg agcaacaaat cgctcacgaa ctgcaactga
     7681 agaatgtgaa aactggcacg ctcgcggaga ttgggcaact agctgatctc gcggtagtcg
     7741 ttggtggcga cggtaatatg ctgggcgcgg cacgcacact cgcccgttac gatattaaag
     7801 ttattggaat caaccgtggc aacctgggtt tcctgactga ccttgacccc gataacgccc
     7861 agcaacagtt agccgatgta ctggaaggcc actacatcag cgagaaacgt tttttgctgg
     7921 aagcgcaagt ctgtcagcaa gattgccaga aacgcatcag caccgcgata aatgaagtgg
     7981 tgcttcatcc aggcaaagtg gcacatatga ttgagttcga agtgtatatc gacgagatct
     8041 ttgcgttttc tcagcgatct gatggactaa ttatttcgac gccaacaggc tccaccgcct
     8101 attccctctc tgcaggcggt cctattctga ccccctctct ggatgcgatt accctggtgc
     8161 ccatgttccc gcatacgttg tcagcacgac cactggtcat aaacagcagc agcacgatcc
     8221 gtctgcgttt ttcgcatcgc cgtaacgacc tggaaatcag ttgcgacagc cagatagcac
     8281 tgccgattca ggaaggtgaa gatgtcctga ttcgtcgctg tgattaccat ctgaatctga
     8341 ttcatccgaa agattacagt tatttcaaca cattaagcac caagctcggc tggtcaaaaa
     8401 aattattcta attttacgcc agcctcttta ctgtatataa aaccagttta tactgtacgc
     8461 aataacagta atggtttttc atacaggaaa acgactatgt tggcacaact gaccatcagc
     8521 aactttgcta tcgttcgtga gcttgagatt gattttcata gcggcatgac cgtaataact
     8581 ggcgagaccg gcgcgggtaa atctattgca atagatgccc tcggtctttg tctcggtggt
     8641 cgcgctgaag ccgacatggt gcgtaccggc gctgctcgcg ctgacctgtg cgcccgtttt
     8701 tctctgaaag atacgccagc ggctctgcgc tggctggaag aaaatcagct tgaagacggg
     8761 catgaatgtt tgcttcgtcg cgtaatcagc agcgatggtc gctcccgtgg tttcatcaac
     8821 ggtacagctg ttcctctgtc acaactgcgc gaactgggtc agttgctgat tcagatccat
     8881 ggtcagcacg ctcatcaatt actcaccaaa cctgagcacc aaaaattcct gcttgatggc
     8941 tatgccaatg aaacctctct actgcaggaa atgaccgcac gttatcagtt gtggcatcaa
     9001 agctgccgtg acctcgcgca tcatcaacag ttaagtcagg aacgcgccgc ccgtgcggaa
     9061 ctgctgcaat accaattaaa agaacttaac gaatttaatc cgcagcccgg agagtttgaa
     9121 caaatcgacg aagagtacaa acgtctggcg aacagcggtc aattgctgac caccagccag
     9181 aatgcattgg cattaatggc ctacggtgaa gacgcaaacc tgcaaagtca gctttacacg
     9241 gctaaacaac tggtgagcga attgattggc atggacagca aactgtccgg cgtacttgat
     9301 atgctggaag aagctaccat ccagattgct gaagccagcg atgaactgcg ccactactgc
     9361 gatcgtctgg atctcgatcc caaccgacta tttgaacttg aacagcgcat ctcaaaacag
     9421 atttcgctgg cacgtaaaca tcacgtcagc cctgaggcat tgccacagta ttaccagtcg
     9481 ctactggaag aacagcagca actggacgat caggccgact cacaagaaac gcttgcgctg
     9541 gcggtaacga aacatcatca gcaggcactg gaaatcgcgc gcgcattaca ccaacaacgc
     9601 cagcaatatg cagaagaact tgcacagctg atcaccgaca gtatgcatgc gctctcaatg
     9661 ccgcatgggc agtttacgat cgatgttaaa tttgacgagc atcacctggg cgctgacggt
     9721 gccgatcgta ttgagtttcg ggtaaccacc aacccaggtc agccaatgca gcctattgcc
     9781 aaagtcgcat ccggtggtga attgtcccgc atcgcactgg caacccaggt catcacggcg
     9841 cgtaaaatgg aaaccccggc actgattttt gatgaagtgg atgtagggat tagcggtcca
     9901 acagcggcag ttgtcggcaa actgctgcgt caactcggcg aatcaactca ggtgatgtgt
     9961 gttacccacc tgccacaagt cgcgggatgt ggtcatcaac actattttgt cagcaaagaa
    10021 accgatggtg cgatgacaga aacgcatatg caatccctga ataaaaaagc gcggttacaa
    10081 gagctggcgc gcctgcttgg tggcagtgaa gtcacacgta atacactggc gaatgcgaaa
    10141 gaactgcttg cagcgtaaac ttttttcctg cttcacggtc agagtaaaca gcaaaacgcc
    10201 gtaagaccgg aaagcaaaag gttttaaagt gatgaaaggt ctattatcat cggcatatta
    10261 cagatgagcc acgtactgct agggcccgaa aaggaatcaa atcactatgc gctgtaaaac
    10321 gctgactgct gcagcagcag tactattgat gttgaccgca ggctgttcca ctctggagcg
    10381 agtggtttac cgtcctgaca tcaaccaggg gaactatctg accgctaacg acgtatccaa
    10441 aatacgtgtt ggcatgacgc aacaacaagt tgcgtacgca ttgggtacac cgctgatgtc
    10501 cgatccattt ggtacgaata cctggttcta tgtcttccgc cagcaaccag gtcatgaagg
    10561 tgtaacgcag caaacgctga cgctgacctt taacagtagc ggtgtgttga ccaatattga
    10621 taacaaacct gcgctgagtg gtaactaata ataaagttgc tctcaaagac gttaaaaaag
    10681 gtgctcaatg agcacctttt ttctgtctgt tatttattcg ctgatttttc tgctcgttgc
    10741 ctgcgaagct ctttcggatc ggcaatgaga gggcgataaa tctccacccg atcgccatca
    10801 tgcacactat cgcttagttt tgccggacgg ctgtaaatgc cgactttgtt tttagttaaa
    10861 tcgatatcgg tacgcaattc cagcaagcca ctggcgcgaa tagcttcttc aaccgtcgcg
    10921 ccctcctgca gcgtcacgcg ctgcaggtac tgcttctcag gtagcgcata agccacctca
    10981 acggcaattt tacctggcac tgtaaacctc tttcgcacga accgtaaaag cctggaccat
    11041 attagacgcc agctctttaa acacgcgacc aaaggcgagt tcaatcaact tattggtaaa
    11101 ctcaaagtcg agatgaaact cgatacgaca cgcctcctgg ctcagcggcg taaacttcca
    11161 tccaccaatc aatttcttga acggcccatc caccagattc ataagaatac tttggttact
    11221 ggtcaactgg ttgcgggtag taaacgtttt gctgatccca gccttagaga catctaccgc
    11281 cgcagtcatc tgcccaggag tggactccag aatccgactt ccggtacaac ccggcaaaaa
    11341 ctgaggataa gactgaacgt cattcactaa ctgatacatt tgctccgcgc tgtagggtac
    11401 cagtgcggtc cggctaatct gaggcataac tatttccatc aacaaaaatc caacaaataa
    11461 tatcatttat cctgctgtaa aaaaaacgct atcccggcgc tgggtaacat cgggttcatg
    11521 ctaagataga gccttgtccc ccgcaggatt gatatggggt gttttcgatt tcagattacc
    11581 gatgattcac gacgcttatg acgaagaaaa aagcacataa acctggttca gcgaccatcg
    11641 cgcttaacaa gcgcgcccgt cacgaatact ttatcgaaga agagttcgaa gcgggacttg
    11701 ccctgcaagg ctgggaagtt aaatccctgc gcgcaggaaa agccaatatc agcgacagct
    11761 acgtccttct gcgtgacgga gaggcatttc tgtttggcgc taacatcacg ccaatggccg
    11821 tggcctccac gcatgtggtg tgcgatccta cccgtacccg caagttactt ctcaaccagc
    11881 gcgaactgga ctcattgtac ggtcgcgtca atcgagaagg ctataccgta gtggcgctct
    11941 ccctgtactg gaaaaatgcc tggtgcaaag tgaaaatcgg cgtcgccaaa ggtaaaaaac
    12001 agcacgataa acgttcagat atcaaagaac gcgaatggca ggtggataaa gcacgtatca
    12061 tgaaaaacgc ccaccgttaa acctgcactc caattattga ccagttcctc accgcgcctc
    12121 cctctccggc ggcgcgaatg aacatcttat tggctatcac atccgacaca aatgttgcca
    12181 tcccattgct taatcgaata aaaatcaggc tacatgggtg ctaaatcttt aacgataacg
    12241 ccattgaggc tggtcatggc gctcataaat ctggtatact tgccgttaca cattggggct
    12301 gattctggat tcgacgggat ttgcgaaacc caaggtgcat gccgaggggc ggttggcctc
    12361 gtaaaaagcc gcaaaaaata gtcgcaaacg acgaaaacta cgctttagca gcttaataac
    12421 ctgcttagag ccctctctcc ctagcctccg ctcttaggac ggggatcaag agaggtcaaa
    12481 cccaaaagag atcgcgtgga agccctgcct ggggttgaag cgttaaaact taatcaggct
    12541 agtttgttag tggcgtgtcc gtccgcagct ggcaagcgaa tgtaaagact gactaagcat
    12601 gtagtaccga ggacgtagga atttcggacg cgggttcaac tcccgccagc tccaccaatc
    12661 atgattggac ggtataagga caacaccaat aaaaacagga ggttagcagt ctcagcagga
    12721 caccgaccag acggtgaaga gacataaaag gatacgcaaa ggagccgcgg ctcctggtga
    12781 catgaaagcc cacagatgtg ggctttttcg ttgatggtca gaacgaccag ttcacaccag
    12841 ctaccccgtt ccacggggat tccacaccgg caccatggct ataccccacc ccaagatgcc
    12901 cgcttaacgt actgctgaat gaggctttaa tacctgcctg gtatattcca cgtctgcccg
    12961 acaaatcatt gacgaaatta ccgtcactat tcactttcac ccggttatca tcgacaaatt
    13021 ctttgcgcac agccgccttc agccacggct caacttccat accgttcccc agacgcatgt
    13081 tgtaactcag cgttgcaccc agttcacgat ataaacctgc gggtatcgac tgatttcgat
    13141 tccatgccat tggataaatg atattcaggg ttatcagcgg tgaccccccg ttaacgatgc
    13201 atacggcgtc aggttccagt taccatcggt aaatcgcatc ccggtttcaa tgtgaccgcc
    13261 cagcccgttg ctgcgataac tgccgttggt ggctccaccg ctgccaaccg tggagtcgcg
    13321 agcttcggta cctgctaata ccaataaatt accgccattt tccagcaaca tattggtcgc
    13381 taaattgccg gaaatggaaa aagtgccgta ctggtaagta ccgctgattt caataccgtt
    13441 agccgtgctc gtctggagag cggcaccgct gttctggacg atatctgtcg ctttgccatt
    13501 atcgttaact gtcagcgtac cgccttcatt gatctttgtt tttattgcct ctccgttagc
    13561 tgaaactgtt tgtattccgc cgtcgttaat cgttgtctca ttcgccaccc cctcgacgat
    13621 ttgttcgccg ccggtgagcg tcgtgcctgt cgcagtggct tttgttttga cgatctcccg
    13681 tccgcccgta ttgacctgtg ttttgtcaga agaggtgtct gactccacgg ttaacacgcc
    13741 gccatttgcc agcaggatat tgttcgccgc accctgctcg atgctgaacg cgacgccatc
    13801 cgcgcgtgtt cctgtgaccc gcgtcgccct ggtggttgca accaaagcgc cctggctact
    13861 ctgctgtatc cccgttgcgc tgcctttctc ccgcacatcg agtgtgccgc cgtcattaag
    13921 caccgagttt tcagccagac cgccctcatt aactacctgt gaacccccat taataatgga
    13981 accttccgct gtcccgtttg ccataatttg ttgtaggcca gagacgatat cggtattgat
    14041 tgccttacca taattctgaa cggtttgcgt gccaccattg atgtgtgttt tctctgttga
    14101 cccaccatca acaatttgtt caccactttc gatatttgcc tcagtggcta atccatatac
    14161 cgtttgcttg ccacctttga tatttgcttt atcagaagtg gcactggcat atattgtttg
    14221 ggtgccagca ctattgagta cagtgccaac atcttttcca taaacatcca ttttgccgtt
    14281 ggcattaata atcgtatcaa ctgcccggga accagtgacg actgttaatg agccagcgtt
    14341 ttccagcact acatttttag cttctgaatt cctgatgtag aaagcatcac cataactgtt
    14401 ggttcctttg ataagggttc cggaagttgt ggaagcaatt aatgcgccgc cggattgttg
    14461 ctcaacatgc ttagcctcac caccgtcctg aacctccaga acgccaccat tattaagtct
    14521 ggtggtatct gttttagcct ccttctggac aatcag
//
LOCUS       DAJTOR010000097        14547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTOR010000097 DAJTOR010000000
VERSION     DAJTOR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.097"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..460
                     /locus_tag="OO249_002612"
     CDS             <1..460
                     /locus_tag="OO249_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5636864.1"
                     /translation="INFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            830..1654
                     /gene="gadX"
                     /locus_tag="OO249_002613"
     CDS             830..1654
                     /gene="gadX"
                     /locus_tag="OO249_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5636865.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2022..2750
                     /gene="gadW"
                     /locus_tag="OO249_002614"
     CDS             2022..2750
                     /gene="gadW"
                     /locus_tag="OO249_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5636866.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSLGTFS"
     gene            complement(3113..6226)
                     /locus_tag="OO249_002615"
     CDS             complement(3113..6226)
                     /locus_tag="OO249_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5636867.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6251..7408)
                     /gene="mdtE"
                     /locus_tag="OO249_002616"
     CDS             complement(6251..7408)
                     /gene="mdtE"
                     /locus_tag="OO249_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR5636868.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7468..7746
                     /locus_tag="OO249_002617"
     CDS             7468..7746
                     /locus_tag="OO249_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636869.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7747..8274)
                     /gene="gadE"
                     /locus_tag="OO249_002618"
     CDS             complement(7747..8274)
                     /gene="gadE"
                     /locus_tag="OO249_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5636870.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9073..9645)
                     /gene="hdeD"
                     /locus_tag="OO249_002619"
     CDS             complement(9073..9645)
                     /gene="hdeD"
                     /locus_tag="OO249_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5636871.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9900..10232
                     /gene="hdeA"
                     /locus_tag="OO249_002620"
     CDS             9900..10232
                     /gene="hdeA"
                     /locus_tag="OO249_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5636872.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10348..10674
                     /gene="hdeB"
                     /locus_tag="OO249_002621"
     CDS             10348..10674
                     /gene="hdeB"
                     /locus_tag="OO249_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5636873.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10738..11385
                     /locus_tag="OO249_002622"
                     /pseudo
     CDS             10738..11385
                     /locus_tag="OO249_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(11427..11957)
                     /locus_tag="OO249_002623"
     CDS             complement(11427..11957)
                     /locus_tag="OO249_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5636874.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12113..12679)
                     /gene="slp"
                     /locus_tag="OO249_002624"
     CDS             complement(12113..12679)
                     /gene="slp"
                     /locus_tag="OO249_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5636875.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(13034..14151)
                     /locus_tag="OO249_002625"
                     /pseudo
     CDS             complement(13034..14151)
                     /locus_tag="OO249_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4012 a         3113 c         3247 g         4175 t
ORIGIN      
        1 catcaacttc tcccgcccgg cgggtcaggt aattgcacag tactatgaat tcctgcgcct
       61 cggtcgtgaa ggctatacca aagtacagaa cgcctcttac caggttgccg cttatctggc
      121 ggatgaaatc gccaaactgg ggccgtatga gttcatctgt acgggtcgcc cggacgaagg
      181 catcccggcg gtttgcttca aactgaaaga tggtgaagat ccgggataca ccctgtacga
      241 cctctctgaa cgtctgcgtc tgcgcggctg gcaggttccg gccttcactc tcggcggtga
      301 agccaccgac atcgtggtga tgcgtattat gtgtcgtcgc ggcttcgaaa tggactttgc
      361 tgaactgttg ctggaagact acaaagcctc cctgaaatat ctcagcgatc acccgaaact
      421 gcagggtatt gcccagcaga acagctttaa acacacctga taacataacg ttgtaaaaac
      481 cgaatgccca gcctttaaaa aaacagctgg gcattcggtt gcttattaat cgcaataata
      541 tattggctgt ctattcatcg tgttgataag atatatgtta tatgaatgtt tattaaatgt
      601 agcgattgcc tttaatgtca cgaaaaggga ttatttgctt gctattaatt tcactgcgtt
      661 cttaaccgcg tttttgtatt ataaatccag tcatcctgcc cgtagaacat cacacattat
      721 catcctgttc tcccgcttcg tttaaattta tttatcaatc aatttgactt aagagggcgg
      781 cgtgctacat taattaacag taatatgttt atgtaatatt aagtcaacta tgcaatcatt
      841 acatgggaat tgtctaattg cgtacgcaag acataaatat attctcacca tggttaatgg
      901 tgaatatcgc tattttaatg gtggtgacct ggtttttgcg gatgcaagcc aaattcgagt
      961 agataagtgt gttgaaaatt ttgtattagt atcaagggat acgctttcat tatttctgcc
     1021 gatgctcaag gaggaggcat taaatcttca tgcacataaa aaaatttctt cattactcgt
     1081 tcatcactgt agcagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa
     1141 gaatcttcgc tatgcagaaa tgctacgtaa aagagcatta atctttgcgt tgttatctgt
     1201 ttttcttgag gatgagcact ttataccgct gcttttgaac gttttgcaac cgaacatgcg
     1261 aacaagagtt tgtacggtta tcaataataa tatcgcccat gagtggacac tagcccgaat
     1321 cgccagcgag ctgttgatga gtccaagcct gttaaagaaa aaattgcgcg aagaagagac
     1381 atcatattca cagttgctta ctgagtgtag aatgcaacgt gctttgcaac ttattgttat
     1441 acatggcttt tcaattaagc gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt
     1501 catttacgtc tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc
     1561 gcagggattg ccgaaccgtg actcggcggc aagtattgtt gcgcaaggaa atttttacgg
     1621 cactgaccgt tctgcggaag gaataagatt atagaatttt actcagacat aaaaaaaacc
     1681 tggcataggg gaccgggaag aggatagtct gccgtctcca gactaataaa ccgctataac
     1741 actccctgtt ggcacgggaa actttgtgct ctcagtaagt taaatataac ttttactgga
     1801 aataagatca gccatttttt tataaacata agctatacgc tgtgcgaaaa tatattcgtg
     1861 ctgcatttac ttattatcaa ttaactgtta tgcaaaacta ctttgtggat aaattttggt
     1921 cctaccaaat ctggcagttt ttgcgctaag aaacagtctg gcaacatttc attagtatac
     1981 tgaaattgaa ataatcgcag tatgaaatat aagggataat catgactcat gtctgctcgg
     2041 tgatcctcat tcgtcgttca ttcgatattt atcatgaaca gcataaaata tcgctgcata
     2101 acgagagtat cgtgctgctg gagaaaaatt tggcagacga ttttgcgttt tgttcaccgg
     2161 atacgcgacg actggatatc gatgagctga cagtttgcca ttacttacaa aatattcgtc
     2221 agctaccacg caatttaggg ttacatagca aagaccgtct attaattaac cagtcacccc
     2281 ccatgccgct ggtgacggcg atttttgata gcttcaatga atccggggta aattcaccga
     2341 tactgagcaa tatgctctac ctttcctgtt tatcgatgtt ttctcataag aaagaactga
     2401 tccccttact tttcaatagc atcagcactg tttcaggaaa agttgaacgc cttattagct
     2461 ttgatatcgc caaacgttgg tatctgcgcg atatcgcgga aagaatgtat accagcgaga
     2521 gtctcatcaa aaaaaagttg caggatgaaa atacctgttt cagtaaaata ttactcgctt
     2581 ccaggatgtc gatggccaga cgattactcg agttacgtca aattcctctg catactattg
     2641 cagaaaaatg tggctatagc agtacgtcgt actttataaa cacatttcga caatattatg
     2701 gtgtaacgcc acatcagttt gcgcaacatt cgctaggtac cttttcctga catattttgc
     2761 atttgaatat tggtcaggat ctcacacctg cttcatgtga aactcttccc tgatgatttc
     2821 tgccgggcta ccggctagtt ctctttcgca gtttgccagg cggtataggt attttcagga
     2881 ggaaaagact cggcatgttt gggattatta agctgacaat tcatacgatt acgggaatgt
     2941 gggtcagtat tgtgctgttc aaattgatga cgaatggctg gagtggtttc tactttcagt
     3001 gctgtgtact aagcctggta ttcctgacgg ttagctggtt gttgagtggt gaatggttag
     3061 caggaaagag taaggctgaa ccttcacgtt caaccttact ctcatttaca cgttacgctt
     3121 ttttaaagcg ggcaaagaga tgttccacta caacgaaaaa gaccggaacg aagtaaattg
     3181 ccagcactgt tgcggcaaac atcccgccca tcacgccggt acctaccgcg ttttgcgcgc
     3241 cagaaccggc accatgactg ataaccagcg gcagcacgcc gagaataaag gccagagagg
     3301 tcatcaggat tgggcgtaaa cgcatccgcg ccgcttcgat gattgcccct atcggcgttt
     3361 tcccttcttt ctgcatcatc tcaacggcaa attcgacaat caggatggcg ttcttggcgg
     3421 aaagcccgat ggtggtcagc aaaccaacct ggaagtagac gtcattactt aagccgcgca
     3481 gatcggtggc cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc accgagaacg
     3541 gaattgacca gctctcatag agtgcggcga gggcgaggaa caccacgacc agtgaaatgg
     3601 catacagtgc aggagcctgg tttgaggata acgcttcctg atacgacagt ccggtccatg
     3661 agtagccgac gcctgccgga agtttagcga ccaggtctgc cataaatttc atagcgtcac
     3721 cggtactttt cccggccgcc gcttcaccta aaatctccat tgacgggatg ccgttgtagc
     3781 gttccaatcg cggtgaacca taggtccatt cggtagacga gtaggcagaa agcggtgcca
     3841 tcgtgccaga ggcgttgcgt acataccatt ggttgatgtt atccggcaac atacggaacg
     3901 gcgtgcctgc ctggacatac acttttttca cccgcccctg gttgaggaag tcgttcacgt
     3961 agctgctgcc gaaggcggtg gaaattgtct ggttgatatc agacagcgcc acacccattg
     4021 cctcagcttt cgcggcgttg acgttcactt tgaacatcgg cgtatcttcc aggccgttcg
     4081 ggcgtacccc ggtgacctgg tcaggcgatt gcgctgccag tgataacagc tcgtttcgtg
     4141 cctgggttag tttttcgtgc cccaggttac cgttgtccag cagttccata tcaaaacctg
     4201 acgcggtacc cagttcagcc accgcgggta agttgaacgg gaagacgacg gctttattga
     4261 tactgcttaa cgcaatcatt gcccgctgaa tgatcgcggt aaccgagttt tcctcaccga
     4321 cacgttcaga ccacggcttg agactgataa acgccaggcc gttgttttgc ccctggccgc
     4381 tgaagccaaa gccgccaacg gtaaacaccg actgaacatt atctttctct tttgtaagat
     4441 aataatccgt cacctgttgc agcactttcg tggtgttaac catggtggca ccggaaggta
     4501 actgcgcggt ggtcataaat accccctgat cctcttctgg taagaaagag gtcggtgtac
     4561 gcaggaacag caccgccatc ccggcgcaaa tcagcaggta gaccaccatg tagcgaccgg
     4621 tacaacgcaa cagcgagcgg gtgctatcgg tatagtgctg agttgatttt tcaaacagcg
     4681 tgttgaagcg tgcgaacagg gcgttaggtt tgtgaccgcc ttccggcgcg gctttcagaa
     4741 tggtggcgca cagggcaggg gtcaggctca ttgccacaaa tactgaaagc agcatggagg
     4801 agatcagcgt gatggagaac tggcggtaga tctccccggt tgcgccgctc ataaaggcca
     4861 tcggcataaa cactgcggaa agaacaacag caataccgac cagcgcacgt tggatctgcc
     4921 ccatcgattt atgcgtcgct tccttcggtg gtagcttatc ttccgcaatg acacgctcga
     4981 cgttctccac caccacgatg gcgtcatcca ccagtaaccc tatcgccagc accatcccga
     5041 acatagttaa cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac gtcccgagaa
     5101 taaccaccgg tacggcaatc gtcgggatga ttgtggcacg gaaattctgc aaaaacagat
     5161 acatgaccag gaagacgagg atgatagcct caaccagtgt tttaaaaact tcctgaatag
     5221 aaatttcgat aaacggcgtg gtgtcgtaag gataaaccgt cttcagactt gcggggaaat
     5281 aggctgataa gcggttcagc tcctctttga ctgcccgcga ggtatccagg gcgtttgctc
     5341 cggtagccag tttgatggcg atcccggcag caggtttgcc gttatagcgc gccacggtgg
     5401 aataatcttc cgctccaagt tcgacgcggg cgacatcacg cagcagcact tgcgaaccat
     5461 cttgctgaac tttcaacagg attttgccaa attcttccgg cgtttgcaga cgcgtctgca
     5521 caatgatcga ggcgtttagt tgctggtctg ccgcctgtgg catgccaccc agttgaccac
     5581 cggaaatctg gttgttttgc accttaatct gggaaataac atcggaaggt accagattgt
     5641 atttattgag tttttgcggg tccagccaga tacgcatggc gtactcggaa ccaaagagct
     5701 gtacgctacc aacgcccgcg gtacggctta gcgggtcttt gatattagac gctacatagt
     5761 ccgcgatatc gtactggttg aggctgccgt tatcagaaat aaacgccgct accatcagaa
     5821 tattactgct cgacttatca acgctaatcc cctgctgctg caccgcttca ggtaatgaag
     5881 gcatagcgag ttgcagttta ttttgcacct gaacctgtgc gatatcagga gatgtcccag
     5941 tctcgaaggt cagagtgata gaggcattac ccgccgcatc actggttgaa gacatgtaca
     6001 tcaggccatc aagcccattc atattttgct caatcacctg agtgaccgag tcttctaccg
     6061 tttgcgcatc ggcacctgga taggtagcgc tgacggtaat ggtcggtggc gcaatctgcg
     6121 gatactgcgc aaccggtaag ttcatgatcg ccagaccacc tgcaagcatc ataataatgg
     6181 caagtaccca ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa gtccccttaa
     6241 gcctgcaacg ttattgtttc gattcggtgc tggcgttttc ctggctggag gaaattgctc
     6301 gtgctttgat acccggacga atgcgttgca aaccggaaac gatcacccga tcgccagcct
     6361 gcaagccaga ggtgacaacc cactggtcgc cgatggcttt gctggcttca atttcgcgta
     6421 gctgcacaac atcgtcttta tccagaatga gcgccgttgc tttaccctgg gcgttgtggg
     6481 tgacgccttc ctgcggcacc agtaatacat tctggcggct accttcatcc actaatgccg
     6541 tgacgtacat gccaggcagc aagtcaccat ttgggttggg gaaaatcgcc cgtaacgtca
     6601 cggagcccgt ggtttcatcc accgtcgggt cggagaattt cagtgtgccg gtctggctgt
     6661 agcgtttacc attttccaga ttgagctgta ctggcgtact gccctgaacc tgtttgattt
     6721 gcccactggc gacctcttct ttcatgcgca agaaatcctg caccgactgc gtgagatcga
     6781 cataaatcgg gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta gcggtaacga
     6841 gtgcgccgac ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag gtgacattcg
     6901 cgtattgcag attgatcgtc gcctgttcaa cagccgcttt ggcgacggtg acattggctt
     6961 ctgcttcatt cagctgggtg cgcgcggtgt cgtaatcctg acggctaacg tagttggtct
     7021 tcagcaacga tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta gagagcgctt
     7081 tcgccagcga gcctttggcg gagtttagct cggcctgtaa aggtgcagga tcaatctgat
     7141 acagcgaatc gccctggttc actttatcgc cttcgataaa gttgcgttta atgataatac
     7201 cgcccacctg gggacgtatc tcggcaactt cataaggaac ggttctaccg ggcaattcgc
     7261 tcaacacatt gaccgaaccg ggggagagtg tgacgacacc gacctcaggc gtcatggcgg
     7321 cggcgttttc cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag aataacaacg
     7381 gtattaacag ctttcttctt ctgttcattt tagtccctga aaattcttga gaaataacgg
     7441 ttctttaaat tgcgcgcgag gagagatatg agggatttac agaccagcgg catagtgggg
     7501 ttgtcagcca gtaaggtggg atacaggcac agtgatcgac atggtgaggt caacgacaaa
     7561 aaaaatgttg ccgttctgcc aacagttcct gccagcattc gggcaacgaa gggttctact
     7621 ggtggataca cataccaggg gaataaagtc gttttagcct ctcctttagt aatcaccggg
     7681 ggtaatgaaa tcagcatctg catccctcgt catgccagcc atcaatttca gttgcttatg
     7741 tcctgactaa aaataagatg tgatacccag ggtgacgatg tcgctcatac gtcttagttt
     7801 caacttgtac ataatgttga atttctgaat ctttaatgtt tgttgcgaaa tattgagcgt
     7861 caacgtgata ttgctttcat ttttattttt cagaatcagt tgcataattt tgaactgatt
     7921 atgtgaaaaa cataattgtg aattcttatg gggcaagtgt ttaccataag taatctcaag
     7981 aagatttttg atactttctt tgcggctaac aaaataaata ggcgctttag cttttagttt
     8041 actgatgtga taaggtgcca aaacaataat tctctcggca tctaatttct ccagaaattt
     8101 aatcgcttct tcatcaagga tatgattgtg ataggtatca atgataacct tgaagggttt
     8161 attgcctact tttttgatct ctgacaggga attgattttt atcatttcgt gattatcttt
     8221 caactgccaa aagccctgta aaagaaaaga atctttcgtc atgagaaaaa tcataacttg
     8281 ctccttagcc gttatcgttt cgaacatgtc atccttttca ttatttacat ccttgtccga
     8341 atcgttgttc aatatagtaa acgcctataa gaactttacc cttcgtgaac taatacgtct
     8401 attaacacta atcaatactt cagatttatc catcacgcac tccatatatt gatcctaagt
     8461 aagattcctg gttgttatca gcttgtaaat agtaaacaaa aagttaacgt ttgcggatta
     8521 ctgcccaaga ataagttatc aataaccagt ttgtgatctc tgaagaatat tactaaagtt
     8581 aaaatgtaat ccgatttaaa tatcgagtct ccttgtttcg acttaagctg gcaattggat
     8641 tgccagcttt cttttaacgc attgaaatta cctgagtaaa taacgcattc atgtcttcag
     8701 gtttttatat agcgtctgga gacacggata tttatgcaat gtaatcaacg ccagttgcaa
     8761 aaaaatgaac ccggattact tatttaaccg cccatagcat caacgcttac ggaactcttt
     8821 gttcactttc ccacagcact tttaaaacat taaacctgac tacggaaaat atcagccatg
     8881 ataatgtttg aaatggctaa tttgccatgg agtgaaaaaa attagatgaa attcagtagg
     8941 ttgaaataat cactagcagg taattatttc aatgatagtg cgcaattgat ctacaacact
     9001 gcgtagcgga gagagtatta atcggatcat agtcacatca agtgactatg atccgggtga
     9061 caaccggggt aattattgct gcttaacgaa caaactggcg aagctgaaca ggctggcggc
     9121 gctaaatatc agttcaattc ccaccagtgt ggaaaccagc gttacagaca ccatcggcgt
     9181 tgcaccaagg aatatccagg caatgacgat atccagcaca ccaataacga gctgtagcca
     9241 gctgcctttc attgaacgct gacgatacca actcatcagg cgaataaccc ctgcaacaca
     9301 gaacaaaccg gcaataaatg ccgcaatggc aaaaatgccc agctccggtg cgcggatgaa
     9361 gaaatagccg atcaataaat aggcgactgc gacgaggaaa ccggataata ccggccagaa
     9421 attatgactg cggttgctga ataacccgac aataagcgca atacccgagc agattaataa
     9481 tgcacccact actgtgctta aaatatcgcc agagacgaac gggaaactga tacacagcaa
     9541 cccgacgata aacagcagca cggcaataaa ctggattgct ctgcgatgtt ttttaagcat
     9601 ctccagatca aacttcaaaa ttgttgcctt atctatatat aacatagaac caccctataa
     9661 aattaagaag aaaatcccct gctatcaatc tatgccaaaa acgcgtctaa gaatgcagtc
     9721 gatttaataa aaatttccta attgcagtat ctgatgcatc tgtaactcat tgtattgaaa
     9781 taaaaatatc tgattttgat attttccatc aacatgacat atacagaaaa ccaggttata
     9841 acctcagtgt cgaaattgat ttgtgacggc tctttcactt tatagttgag gatattacga
     9901 tgaaaaaagt attaggcgtt attcttggtg gtctgcttct tctgccagtt gtgagcaatg
     9961 cagcggatgc gcaaaaagca gctgataaca aaaaaccggt caactcctgg acctgtgaag
    10021 atttcctggc tgtggacgaa tccttccagc caactgcagt tggttttgct gaagcgctga
    10081 acaacaaaga taaaccagaa gatgcggttt tagatgttca gggtattgca accgtaaccc
    10141 cagctatcgt tcaggcttgt actcaggata aacaagccaa ctttaaagat aaagttaaag
    10201 gcgaatggga caaaattaag aaagatatgt aattccggga atgcgttaca tcgtacttcc
    10261 ttgcatattg aacaggccgg aatatcttct ttaaaagcag ctattcctcc tgttcatata
    10321 taatctatat attgaatggg ttacaaaatg aatatttcat ctctccgtaa agcgtttatt
    10381 tttatgggcg ctgtagcggc tttgtcactg gtgaacgcac aatctgcgct ggcagccaat
    10441 gaatccgcta aagatatgac ctgccaggaa tttattgatc tgaatccaaa agcaatgacc
    10501 ccggttgcat ggtggatgct gcatgaagaa acagtatata aaggtggcga taccgttact
    10561 ttaaatgaaa ccgatctcac tcaaattcct aaagtgatcg aatactgtaa gaaaaacccg
    10621 cagaaaaatt tgtatacctt caaaaatcaa gcatctaatg acttgccgaa ttaatgaggt
    10681 acaagtaaaa aggagtagca agttgagcca tcttgctgct cctttttgca tttttatatg
    10741 acagcagaat ttattatacg tcttatactc gcggcaattg cctgtggcgc tattggcatg
    10801 gaaaggcaaa tgtgaggcaa aggagcaggg ttacgcacac atgtattaat tggcatggga
    10861 agcgccctgt ttatgattgt ttcgaaatat ggttttgctg acgtgctgtc tttagatcac
    10921 gtcggactcg accccagccg tattgctgct caggtggtga cgggcgtcgg gtttatcggt
    10981 gcaggtaaca ttctcgttcg taaccaaaat attgtaggtt tgacgacggc agcggatatc
    11041 tggatgaccg ccgctatagg tatggttatt ggcagtggta tgtacgaatt gggtatgtat
    11101 ggttcagtga tgaccttgct ggtgctggaa gtcttccatc aattaacctt ccgcctgatg
    11161 aacaaaaatt accatttaca gctgacatta gtgaacggta atacggtatc aatgctcgac
    11221 tggtttaagc agcaaaaaat aaaaacggat ttagtttcgt tgcaggaaaa tgaagatcat
    11281 gaagtggtcg ccattgatat ccagcttcat gccacaacgt cgatagaaga tctgttgcga
    11341 ttattaaaag gtatggcagg ggtgaaaggc gtttcaatta gctaatctgt gtggcagcgt
    11401 agtcagactc accgtaagcc tgaaattcac accagataat caatatgctg atggcgtacc
    11461 agctcattga tccgcttaag attgaggatc atcatgatat tgtgtttatg gcagtagaac
    11521 gtttttagcg ttattttcag tttctccaga atatcctccg ttgtcatttg attcgcaatg
    11581 cagctcagaa cctgatgttg cttgcgcgaa agtgacacag ccgcttcagg cacagagcag
    11641 taactgccat tgatgagtgc gacaaaatcg atgatagtga tagtccgcgg aacaaaggtc
    11701 accggcactt tccccaggca gcgtttaata ttaaaggggg agaaaataat cacatggaca
    11761 ggttttaatc gttcaatttg agtgagaaaa ttagaatgaa atacattatt cattaatgta
    11821 tcaataatga caaaggcatt ctgatgcaac attccgtcga tctcttcttc gttctgtata
    11881 tgaacgacat tacctttgct caaaatgttt ttcatcgcgg tgaagaacat cgtatccctg
    11941 gtaattataa gaaacataac cgactcctgg tgcggacatt aattttataa tcagtttgcc
    12001 aaaggtaatt aacaggacag ggcttataat aataatagtt agcttgctgt gtattaattt
    12061 tatgtaatgc acgatgcaga tgctgagaaa gcatctgcat ctttcggtgg tgttatttga
    12121 ccagctcagg tgttacctga ctcaccgcat tggtgtagta aggcgcaccc cagcccggtt
    12181 ccggccagaa tgcaccatag ccgtaatccc ataggttata ggtggtatta accacttctc
    12241 tcaaatgcca cacctggatg ccctgcatat tcacttccag gaagttatac gggactttat
    12301 tgataaagcc aggttgttca ccctgaatgg taccgaggat ggtaacaaag tgattacgat
    12361 agttcactgg gtcaaggaag ccgctttgtc tggcgagcag tcggccctga tagttggctt
    12421 caatatcagg tttcgcatag ctatccaacg gtaatacagc gatttctaac aacgtatccg
    12481 ttttgccatt gataacgttg ataaccttcc caccaaagcg cgcttgttga ccaacatata
    12541 accccggctg gttatgaaca gcaacaaaac ttttttgaat atcaggttgg ttattgcctt
    12601 tgatattttg cggaattgaa ctacatgcgg caagcaaaaa tgaaaggctg aggatgagtg
    12661 caccttttgt catgttcatg ttactatcct tatcaacaaa tattaaaacc ataatagata
    12721 tttctttata gttttcatct gattctgagg cgatatgcct gatagtaacg taaacctgtc
    12781 tgatgtttat atcccttttg tttagttcaa ttccattgat tttggggcgt ggcataagta
    12841 tccatcgccc cataatataa tcctttgttt taaaattaca taaaatctat ttttattcaa
    12901 taattataaa gtgctaataa tcaatattat ggtattatat tctgataatg atttgataag
    12961 cgaattcttt tgccagatgg tatttctatt tccaccccat aatttatatc tttttcatta
    13021 ggaagtggcc tctttaaata aggcgtgtgt gacatttttt ttgggttaag cgtctcgaat
    13081 tgaaatactt cgaactgata ttcagacatt tctgcccatg tttgaatttt attgtatcga
    13141 gagagtgtcg tagaggcttt aacttgctta taacattgtt tttggaattc ttgctgatat
    13201 tttaatatgt cattgtatga aagattggtt atcctggact ctaaaaattt aattattgtc
    13261 gaagcaattt ccgcttttgc tcgtaaaaag ttgtcattaa tggttaataa tgttgaaaat
    13321 aaaccttcga tttgtttgtc aagatattgt ccatctttat ataatttggg ggggtagggt
    13381 gttctttatg taaaaaatag tgtcacctaa atttgcgtca gtgaaattta tattaagtag
    13441 atcttcgaaa taaaaccttg acaaggttag agcgaacccg gaaaggttct gtcctgtcat
    13501 attgaaatca ataaagttgg ttccggcaaa gactttcatc ctgtttttat tttcactgtg
    13561 ctcaatatat aacttcgtca ttttttgata caggctttga atatctttta tattagttgg
    13621 taagtcagct cgttgttcag caataaaatt agtatctgaa aatatggttt ggacactgaa
    13681 tgttatgggt attccctctt ctacgctaat tttagttatg attgccttgt cgggtttagg
    13741 attagcgaca acaatgattc gacattcagc acttttgttt ttttcatatc taatgttatt
    13801 aatctgatta agtattgatt gtgtctgttg cgtaagtggt gtcgaactta tcgtttcaat
    13861 gttattaata agacttacaa gttgaaccac aacttttgag tagtctgcat ttgtatatgt
    13921 ggtgttcgtg tggcatgcat taatttgttg gagttgttgt tgcgctaaat caaattgttc
    13981 agggttgtat ccttctccag agaatgtgga attaatgggt tgttgttctt cccaattatc
    14041 acaaggccac ttcggtgtgt ctggcgtttt aacattatcg gtatttgttg tgcagttata
    14101 aatattgccg tgatatgtat ttattatacc tggggtaaag tcaattgaca taacattcct
    14161 tattcatcat ctgttttatg ctaaggtttt taaattggat aattagaaca ggaatggatt
    14221 ttcttctaca cacaataaat ataatgcgtt atatcttcct gatataatta cctttaaatt
    14281 ccctttcatt tccacccggt aatatttgtg tataaaaaaa gcagtgatag tatcgtaaag
    14341 gaaaacgtta acaactatca aaaagcgctc attttaaaaa gttattatgt tctccgatag
    14401 tgtgtgaagt aatatttttg tgatgtttta gatgtgtaat aatgaaccag gttaaagtgt
    14461 atttaaaaat gtgttgtatt agcgactgaa atctgccttt taaaaaggag gagggtactg
    14521 agacaaagag tcattgcttc tccgata
//
LOCUS       DAJTOR010000098        14369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTOR010000098 DAJTOR010000000
VERSION     DAJTOR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.098"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..733)
                     /gene="fimH"
                     /locus_tag="OO249_002626"
     CDS             complement(<1..733)
                     /gene="fimH"
                     /locus_tag="OO249_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5636876.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGV"
     gene            complement(753..1256)
                     /gene="fimG"
                     /locus_tag="OO249_002627"
     CDS             complement(753..1256)
                     /gene="fimG"
                     /locus_tag="OO249_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5636877.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGETKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(1269..1799)
                     /gene="fimF"
                     /locus_tag="OO249_002628"
     CDS             complement(1269..1799)
                     /gene="fimF"
                     /locus_tag="OO249_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5636878.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(1809..4445)
                     /gene="fimD"
                     /locus_tag="OO249_002629"
                     /pseudo
     CDS             complement(1809..4445)
                     /gene="fimD"
                     /locus_tag="OO249_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
     gene            complement(4512..5237)
                     /gene="fimC"
                     /locus_tag="OO249_002630"
     CDS             complement(4512..5237)
                     /gene="fimC"
                     /locus_tag="OO249_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5636879.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQFAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(5274..5813)
                     /locus_tag="OO249_002631"
     CDS             complement(5274..5813)
                     /locus_tag="OO249_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5636880.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RWVTGGIANAQAWFSLTYQ"
     gene            complement(5878..6426)
                     /gene="fimA"
                     /locus_tag="OO249_002632"
     CDS             complement(5878..6426)
                     /gene="fimA"
                     /locus_tag="OO249_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5636881.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQIVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
     gene            complement(6907..7503)
                     /gene="fimE"
                     /locus_tag="OO249_002633"
     CDS             complement(6907..7503)
                     /gene="fimE"
                     /locus_tag="OO249_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5636882.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(7981..8583)
                     /gene="fimB"
                     /locus_tag="OO249_002634"
     CDS             complement(7981..8583)
                     /gene="fimB"
                     /locus_tag="OO249_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCR5636883.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTPPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            10039..10755
                     /gene="nanC"
                     /locus_tag="OO249_002635"
     CDS             10039..10755
                     /gene="nanC"
                     /locus_tag="OO249_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5636884.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSSDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            10775..11881
                     /gene="nanM"
                     /locus_tag="OO249_002636"
     CDS             10775..11881
                     /gene="nanM"
                     /locus_tag="OO249_002636"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5636885.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNREGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQHGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            11946..12926
                     /locus_tag="OO249_002637"
     CDS             11946..12926
                     /locus_tag="OO249_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5636886.1"
                     /translation="MDAIISPDYYYVLTIAGQSNAMAYGEGLPLPAREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLSFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLIMGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWREK"
     gene            complement(12934..13056)
                     /locus_tag="OO249_002638"
     CDS             complement(12934..13056)
                     /locus_tag="OO249_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5636887.1"
                     /translation="MISFVFLLLVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            13138..14010
                     /locus_tag="OO249_002639"
     CDS             13138..14010
                     /locus_tag="OO249_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5636888.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGSVERLFWDFDGKIIALPQVRKNYPY
                     EGFVEWHRNEVFRG"
     gene            14055..14234
                     /locus_tag="OO249_002640"
     CDS             14055..14234
                     /locus_tag="OO249_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636889.1"
                     /translation="MQDLAASLQLAFDSGAKRVLLPISWAMDIPTVPAELFTKFQVSF
                     YSEPVDAVYKALDVN"
BASE COUNT         4030 a         3312 c         3140 g         3887 t
ORIGIN      
        1 gtacgccgac gccctgcgct ggtgaaaacg acgcggtatt ggtgaaaatc gagttgcccg
       61 catctgcggt tgtgccggag aggtaatacc ccaggttttg gcttttcgca caataaacgg
      121 taagaggaat tggcactgaa ccagggtagt ccggcagagt aacggtgaca tcacgagcag
      181 aaacatcgca gccgccagta ggcaccacca catcattatt ggcgtaaata ttccacacaa
      241 actggaaatc atcgctgtta tagttgttgg tctgtcgcaa aataagcacg gcaattaatg
      301 agccagcttt aatcgccacc ccgcccgcac tgctcacagg cgtcaaataa agcgccaccg
      361 gccacggctt atccgttctc gaattataaa caacccgcgg cgtttcgctg gtggtcggaa
      421 atggatagct actgccacta tattttacgg tcccggaaaa attagataac acgccgccat
      481 aagccgagcc tcgttgcagt gtgacatagt ctgtaatggt ttccggataa tcgttatggc
      541 aaaagatttg cgtcgaaaga tctacgacca ggttttgccc cacattcacg acaggcgcaa
      601 ggtttacata aacattagcg ctgccaccgc caatagggat agcagtacca ttggcggttt
      661 tacaggcgaa tgaccaggca tttaccgacc agcccatcag cagtacagca aacagggtaa
      721 taactcgttt cattacaatt atctcttcgg gttcagctgt aggtataggt gatgctaatc
      781 actgcctgaa tggttccctg agtggctccg ccatttaccg tcaatgctct gacctgtaac
      841 gggaagtgcg ctgattgtga ggaatcatcc acctgaactg ttttggtttc gccagtattc
      901 aatgtgttgc cactgtcatc ctgtagctct aactggatgt tttgcgcggt cccctggttt
      961 ttataatatc cggtactgtc ggctgccccg ctgaagctgg cagtgaccct cgacgttccc
     1021 accggacaat tagtcaactc aagcgcaaca tcatgccagg ccgatgccgc cccggcagac
     1081 atcagactga aagaataaag atcgccgaga tcaaccgtgg cattggtggt ggaaactgtg
     1141 cacggtttgg cgacgacctt accgttcacc gtgatggtga catcggctgc ctgtattgtc
     1201 gcacttgcga gcgccaatat tgccgccaat aaatacccac gtttgcgcca tttcatgagc
     1261 atctccagtt actgatattc aagagtgaag gtagccgtgg cattgatatg ccccgcagtg
     1321 acaggcacct gtgtcgccat tagccgggcg taaaaattca acgtatttgg tttacccggc
     1381 gtcagggtcg tccacgaaat cgcggacgat ggagcattaa ggggtatctg attttgctgc
     1441 tcattcagaa gctgtattcc cagtcccgaa gccgctgaca ccgtattttc aagtgcaagc
     1501 aggttggcat tgtggctatc tgcaacgccg gtaaacccaa cctttacggc agaaacggcg
     1561 ttaccacagg gtgacagcaa aatacgaaat ggaacgacag gagtcgttgc gccaatgttg
     1621 ttaaattgct tcgccgcgtt ttccatcaga tcaacagtaa aattggttga ttcagcggcc
     1681 acactacagc cgttatctct gacatagccg cggatagtaa tcgtgctatc cgcagccaaa
     1741 acgcgactta ccgccagcca caaaaaagcg cacagaagat aaaaaggttt gtttctcatc
     1801 acgccccctt aacgacattc agctgatagc tgggttaata actgctgctg actctctggt
     1861 ggcagttgat aattggcgat acagtgagca ttttcctctt ctccccattt cacctgaact
     1921 tttcccgcta aaggcattcc gctgaggtaa acctgaccat tatccgcaac aatgccgcta
     1981 ctctggctac tctctgatgt caccatcgcc ccaaacggca gcggcttatt attgtgggtt
     2041 agcgtcatga gcagttttat cccaacgcgc gctttaaact ctgctcgcac gatcgcccca
     2101 cgagtgggaa caacgttagc gaccgcgtta tctaaatcga cgttatcagc cagggtattg
     2161 gtatccagcg ccactctgtt ttcccgatat tcggtggcat aaggcagcac ggcataaccg
     2221 cgccagtcgg tacgcacccc cgtctggttt tcgacttttg catcttttgc gccaggcgct
     2281 ttaacaagta ccaccgtatc gtttaacggc tgccccagcg ttacgccatt ggcatgagcc
     2341 agtaccccac cgctgactcc gtaatagagc tgcttaatat catcgctatg gctgtaaccg
     2401 atattggcat tgccgtaacc accgcgataa ttcagcgtgg cgtagcctgt acttccgcta
     2461 ttaccatcgc ctcccccggc atagccggtt tgcacgctat agctgaggtt gttgtcttcc
     2521 agcaacgtac cgtatacacc agccagattg gtcatccgac cgttgagatc gtgtgacacg
     2581 ctgtagctgg cactggcatg tcgccactga gatttactgt cagaacgcag ccagtggctg
     2641 aaaggaatat tgacgttaag cgctaacatc tgatcccgcc ctttttgcca ggcgtttttc
     2701 gtcaggctat agctgagcgt ccagttgata tcttcgaacg cagtatttaa tccagcctag
     2761 aattgctcat cgacattact cgttccccaa taagtttgat ggctaccact caaatacagt
     2821 gttgatgtgc gcccgagttg ctgagtaacg gtgagctgta atttcccgcg tttgttataa
     2881 gcgaggttgt aatagtcggt gaatttcggc ttaacctgaa taactccatc ctgtgtttcg
     2941 atgttgtagc cattcattcg actgtatgtt gtatcagcga aattaaaata tccgctggtc
     3001 gaataacggt aacccactaa ctgaatattc gtgcctgact cattgagcga tttgttatag
     3061 agaaaacgca ccgattgtcc gtcatgctga ctgtcatcgg gaagtgtgga attagcctgc
     3121 gtcatatcca cagacagagc gcccagtgcc cccatgtttt tcccgatacc gaaattaaaa
     3181 gcacgataac gatccgccag ttgcgttcca ccatatattg tccagccagc cggaaggccg
     3241 tggagtaatg tactttggaa aaagcggggt ttttcctgct gcgtatttcc actacggtat
     3301 tctcctgccg taatggaata acgagtatgc ccttcacgtt gcaaaagcgg gactgacgaa
     3361 tagggtacgg taaaaatctg cgtgctgccg tcagcctctt tgatcgttac ctgcaagtca
     3421 ccactattac ctgcggcata gatatcgttg atggtaaaag gccccggtgg caccgtacta
     3481 ttataaatgt catacccatt ttgtttaata gtgacctgtg cagtaccacg agcaataccg
     3541 tggatcaccg gggcaaatcc tctttggcta tcgggtaaca tattgtcatc tgaggccaat
     3601 tgtgcgccgc gaaagttaat accatcgaaa atatcgccct gggtataacc atcacccagc
     3661 gtcagccggg aacgtaacgg tattatgtct cgctcaagcc aggtattgat atgctgccat
     3721 ttatttttgc tacctgatga tctgtcgcta ctgttataac tccagctggt attgtcgcgt
     3781 aaacaccacg caccaatatt taacccactc tgtaggttta aatatgcata atggctgtta
     3841 cccccaatct gattctgtac actatttccg ctgaaattat aattgagcaa tccggcatta
     3901 ataccgggat cccataactc aggaggaata taaccacgcg cgcgattact cataaatgcc
     3961 tgagggatcg tcaggttcag tcgctgctga ccaacatcca gatgcgcagt agcgtcatgg
     4021 atcattgagg ttaatggcac gcaggcatca tctgccagca gattcatacc ggagacagaa
     4081 gccgcattca gccccatact ggcaagttgc gcgcgtgtca ggcagggaac aatcccttgt
     4141 tcactgtcgc ccgtattaaa tgtgacatca cgcgttgcca tataaccatt attcaaatag
     4201 atatcgacgc gatacgtccc tggcggtaat tcttgcccat tttcaaaacg cgataaatcg
     4261 gccacagcct ggggatcatc cgctaaaaag cgcggattaa aatagagttc ggcagatgac
     4321 aaaggtgcct gtgcggcaaa agcacaggcg acaacgagcc ggacaaaaaa accagccaaa
     4381 cgatgcttac gaatatgcaa gcattgtgtg tttcgctggt aaagtcttaa attcagatat
     4441 gacattattc ctccggttta aaattacccg gcagtcaatt ctttttattc aggcgagaaa
     4501 ttccccctgc gttattccat tacgcccgtc attttggggg taagtgcgcc ataatcattt
     4561 attgttcggt aagtaatatt gcttcctgca tcagaaggca atttaaccgt gctttcgccc
     4621 attggaggca ccaatgcatt ttcaagaacc cgggttccgg cattcaactc tgttaccgtc
     4681 agataatagg gtgtcgggtt aatcagcgtc agagaattcg cgctacgacg aaatcttaat
     4741 ttttctgcgg cctgatcggg tggcaacgct aatttagccg ggcgatagta cagtttaatg
     4801 cggctgataa ttgcgaactg tagcgtattc tcagtcaatt ttgatttatc cattgacgga
     4861 atcgctttaa cattcatcca gaataaactt tcccggtcct gtggcaattg gttatttgtt
     4921 gcatcaagaa tacgcaaggt attctctttt tttcccttca tcgcaaacag aggaggcgtc
     4981 acgataaaac gaccatcctt tacaccatcg gcattttcca tccatgattg aattaaatag
     5041 gtactatttt catcattatt tgtcacggca agttgcactt gtttttgccc tgccggataa
     5101 attacgcgag tcgcacctaa ggccactccc gcttcagcgc gtccggcaac catcattgcc
     5161 atgaacatca ggatacctgc cagcaagcag aatgttattt cctgcgattt ccttacattg
     5221 gcgtttttat tactcactgt cctgttcctg ttatcacatt atctgctgaa caattactga
     5281 taggttaaag agaaccaggc ctgggcattg gcgatgccgc cagtaaccca acgcccggta
     5341 gcacgatatt tggcgatgaa atgtagcgaa gtagagcctg agtaaagccg tttccagttt
     5401 actggaggac gattaatcgg tacgaggttt ccttcatcat caaacaacgc tacgccaata
     5461 ttggtggcta tccctggccc ctctcccacg gaaagcacat ccggattttt accatccgcg
     5521 acaccgtgaa acgccacacc cacacgttca ctcaccaccg tgctacattc ccgtaaatga
     5581 ataacaaaag gcaccggtgc gctatcttcc cctaccgcat gaaaccggtt actgctgatt
     5641 tgccccatat tgaccgtcat ttgtttatca ccggcttcaa tccggcaagt ttccgcaata
     5701 atgacgccct gaaattgcat atttccgccg ggcagcgtag tattccattt atttccggcc
     5761 agagcaaaca ttggcagcaa cgacgctaat agaaataatc tcttcctttt catcgttatt
     5821 tttatcgcac aagggtgggc atccctgccc gtaatgacgt ccctgaacct gggtaggtta
     5881 ttgatactga accttgaagg tcgcatccgc attagctgcg ccaggggttg cgacaccggt
     5941 tgcataataa cgcgcctgga acggaatggt gttagtaccg ttattcaggg ttgtttgctc
     6001 actgaatgtc gcaccatcca gcgtcagcgc agcacccgtt ctgtccagga tctgtacacc
     6061 aacgtttgtt gcgctacccg cagctgaact ctgcagagcc agaacgttgg tatgacccgc
     6121 atcaatcgcc gtacctaaaa aggcaactgc ggctttagat gcaacattgg tatcgcaatc
     6181 attcagctga atgttaaaac cgacagcaga actggttgct ccttcctgtg ccagcgatgc
     6241 ggtacgaacc tgtcctaact gaacgatttg atcaacagag cctgcatcaa ctgcgcaagc
     6301 ggcgttaaca acttcccctt taaagtgaac ggtcccacca ttaaccgtcg tggcagcggc
     6361 cagagccgct gtagaactga gggacagagc cgacagaaca acgattgcca gagttttaat
     6421 tttcatgctg ctttccttta aaaaaactat ttctaaatcg acatgggcag tcgttctgta
     6481 cactttgttt tgtcaacgag tttgactgcc aacactgcac agttttcccc caaaagatga
     6541 aacatttggg gccattttga ctcatagagg aaagcatcgc gcacaaactt tttcagttta
     6601 tttgttggct taatattcta ttgttatctt tatttataga tttttatatt gcatgaggtg
     6661 gtttttggag agaagaatga ggaagatgcg tcgagccaca gaaacgttag ctttacatat
     6721 agcggaggtg atgtgaaatt aatttacaag agaaataatt tacatatcaa acagttagat
     6781 gatttttgtc gttttttaaa atttttatgc ttgagaaaaa atacgtaact tatttatgat
     6841 atggacaatt tggccccaat tgtcttgtat ttatttattt tttaacttta ttatcaataa
     6901 gttaattcaa acctcttctc tttttaattt ttcgtttatg agattatttc tttcccataa
     6961 tccggcaaaa cgagcagcat tactggcggt ataacgtaca gtatggcgaa tatttcgatg
     7021 cccgagataa tcctgaatta aacgagtatc tgcaccacgc tccgccagtt cataaccgca
     7081 agcatgcctt aacatatgag gatgagtctg cgttacggtt ccagcttcaa taccggcatc
     7141 acgaataatg cgataggcct gctggcgaga aagccgactc ccgcggcgag aaataaatat
     7201 ggcgtcggtc cggtcagcgc ctttccagtt agcacgttcc agggtccagc gttccacagc
     7261 ttcacgctca tcaaaacgta acgggtgaac ggtagaaaat ccgttcttca gtcggcgaat
     7321 atttattcga ccttcattaa ggtcaagatc ctgataatgc agatcaagca gttcactaat
     7381 acgcatccca tgccgatatg ccaacagaat aagacaataa tctctggctc ccgttgcccc
     7441 gtaacaaacc gcctgcatca tggcctgaac ttctttaccg gtaagataac gacgtttact
     7501 cacaatagta gtactcctaa ctgagatata tttgaatgaa tacctatagg aaacctcaat
     7561 cggtcaaaat tagcccaata gataaaaaga taaccacaca cgaagtgatg tggctatcag
     7621 tcaattacat taaatatcat acaaaaataa aatatcactg atgtgacaac atctattttc
     7681 attgatttga ttcaatataa caatagattg tagtagatta gcttccagtt gcttaaaaaa
     7741 taaagaaaaa agtgcgaaaa caggagaatt taagtgatgt gtaaatttat gaatgatttt
     7801 gtttatttat tttgaagaac ataaaccatt gtattttgta tggatatggt taacttaatg
     7861 cttattatga taattaagaa ttatacttac aataacatca attaataaat ggttaatcat
     7921 ttttgatata tcgtaagaat aatgtagttt ttaacaccat ccctggtatc tcaactatct
     7981 ctataaaaca gcgtgacgct gtcgtcctct ggctctatcc cagatgccgt aaaaacgccc
     8041 tgcattgctg gcggtatacc agacagtatg acgaatattg cgatgcccaa gataatcctg
     8101 gataagtcgc gtatctattc ccatattcgc caaagcaaaa ccacacgaat ggcgtaacat
     8161 gtgcggatga atctccagtg acaacccggc attaccaccg gaagtcgaga taatatggta
     8221 aaactgttgc cgagaaagcg gattcccctt acgcgataaa aatacccact cgctctcagc
     8281 atgcgggtac gaagtacgga tactcaacca gttttttaaa gcctgaactt ctttattcaa
     8341 tagcgggggc gttgttgaaa agcctttttt taatcgatgg atatatatac actttgcctt
     8401 aagatcaata tccgaaatcc tcaatcgaca aatttcactc gcccggaaac catgaataaa
     8461 acaaagcaaa gtcagacaat aattacgtgc tgcatgaggc ccggtatttg ctgctttaag
     8521 gagtgattcg atttcactat gggtcaggaa gttccttttt ttgttatcag ccttattctt
     8581 catcgttttt cccttataat tacagacgcg cactagctgt gctgggttag caataatcca
     8641 acatttttat cctgattatg tttataaaag cgaacgtttg cttaataact aacgtaagtg
     8701 gaccagttct tctgagtgaa cttaaatggg gtagcaacag ttaattataa taacgttgcc
     8761 atacaaacgc tatgctcttg catgctatgt acctttatat atttatcaat cggatacgaa
     8821 tctaataata cccctcatca ttaatgcaac taatcttatc tatatatcat gtgatatgtt
     8881 ctgtaacaag taaaatcagc ataaaaaatt cagcaaaatt taatattcgt tcaactagcc
     8941 gtctcacata tgacattatt agtcaactcc atttgccaca aaatggctat ttcatggaac
     9001 gcagtaattt cttagtggct attatcatgc taattcttag gtccctagcg attattcctg
     9061 gctgattcgt cagcttacgc catgagattg ttacattcct ttttatcctg gagatatggg
     9121 ggaacgcagg agatgatttt ttcatctaca ttcgttggta aatatagctt tggtcactac
     9181 gacttaaacc ttcctcaatc acagccattc gccgggcgat acggcaaaca aactaagtga
     9241 aacatcatcc tgaaaacacc aacatcaaca agcctctcca gatcgacttc agaagtgacc
     9301 agttacaagc cacaaaccag gaaacatatt tcatgatgag aattatgctc agcctgatgg
     9361 cgggtaaaca ctgattttca ccaagctcac tgagttagct tgcaaagctt cccttgttta
     9421 gactccaaat aaattattta taaaacaaac atttaaacaa agatctttag tttttgaaga
     9481 ccaccgcaag tgttcgtctg gcttcacatg gcatcttcct cttagaaaaa gatcgacata
     9541 ttttgtgaca cgaattgcaa atctggtttt gttgtatgga ttgcgtgatt tttgatctgg
     9601 tataacaggt ataaaggtgc accaagatag tcaatgagac agggcatctc gcaatctatg
     9661 gcaaacatca cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag tctgaaagga
     9721 atatgaaaat gagatcaaca gacactctat tttatgactc tgggtaaaat ggattgagta
     9781 agtgatatag cttacgaaaa ttcaaatcaa ttaaacatca gaagagattt tatactcagg
     9841 tatttaatct ggatctctgt ttatttaaat aatgtgaaaa gagatttttc acaggagacc
     9901 ttatacaaaa aaatataaaa tacagctacc ggttgccaaa gacactataa gcctggcaaa
     9961 aaatattaca caacataaat gctaattgtt tatgcgggct ttgtattgct ttctgtatcc
    10021 tacaaatgag tgaaatttat gaaaaaggct aaaatacttt ctggcgtatt attactgtgc
    10081 ttttcgtccc cattaatttc tcaggctgcg acactggacg tacgtggtgg atatcgtagt
    10141 ggaagccacg cctatgagac tcgactcaaa gtcagtgagg gatggcaaaa tggatggtgg
    10201 gcaagcatgg aaagtaatac ctggaatacc attcatgata ataaaaagga aaatgccgca
    10261 ctcaatgatg ttcaggttga agttaattac gcgattaaac ttgatgatca atggacggtg
    10321 cgcccgggaa tgttaacgca ttttagcagc aacggcacac gctacggacc ctacgtaaaa
    10381 ctgtcctggg acgcgacaaa agatcttaat tttggcattc gctatcgtta cgactggaaa
    10441 gcttaccgac aacaagactt atccagtgat atgtctcgtg ataacgttca tcgttgggat
    10501 ggatatgtca cttaccatat taatagtgat ttcaccttcg catggcaaac gacgctatac
    10561 agcaaacaga acgattatcg ctatgcaaac cataagaaat gggcgacgga aaatgcattt
    10621 gttctacaat accatatgac gcccgatatt acgccataca tagaatatga ctaccttgac
    10681 cgtcagggtg tttacaacgg cagagataat ttatcggaaa acagttatcg cattggtgtg
    10741 tcatttaaac tgtagtagac aggagacagt cacaatgaat aaaacaataa cggcgcttgc
    10801 tatcatgatg gcttcatttg ccgcaaacgc gtctgtatta ccggaaactc ctgtgccatt
    10861 taaaagtggt accggagcaa ttgataacga cactgtctac attggtttag gtagcgcagg
    10921 tacggcatgg tacaagctgg atacacaggc caaagataaa aaatggacag cgttagctgc
    10981 attccctggt ggaccaagag atcaagcaac ctctgcattt attgatggca atctgtatgt
    11041 gtttggcggc attggcaaaa acagagaggg cttgactcag gtatttaatg acgtacacaa
    11101 atacaacccc aaaaccaata gctgggttaa attgatgtcg cacgcaccga tgggcatggc
    11161 gggtcatgtg acttttgtac acaacggcaa ggcttatgtt actggcggtg ttaaccagaa
    11221 tatcttcaat ggctattttg aagatctcaa cgaggctgga aaagattcaa ccgctgtaga
    11281 taaaatcaat gcctactatt ttgacaaaaa agcagaagat tatttcttca ataagtttct
    11341 gttgtctttt gatccctcaa cacagcaatg gagttacgct ggcgaatcgc cctggtacgg
    11401 aacggctggt gcggcggttg tgaataaagg tgataaaacc tggcttatta atggcgaagc
    11461 caaacccgga ttgcgaacgg atgccgtatt tgaacttgat ttcaccggta ataatttaaa
    11521 atggaataag cttgctcccg tctcatcacc agatggcgtc gctggcggtt ttgcggggat
    11581 aagcaatgat tctcttatat ttgccggagg ggccggattc aaaggttcac gagaaaatta
    11641 ccagcacgga aagaactatg cgcatgaagg cctgaaaaaa tcatatagca ctgatattca
    11701 tctttggcat aacgggaaat gggataaatc gggtgaatta tcgcaaggtc gggcctacgg
    11761 agtatcattg ccctggaata atagtctatt gattattggc ggtgaaactg caggcggcaa
    11821 agcggtgacg gattcagttt tgatctctgt gaaggataat aaagtcacag tacaaaacta
    11881 acgcttcagg gccccagtaa aggggccctg ctatcaattt atcatttcaa ttacgcgaaa
    11941 attatatgga cgcaataata tcgcccgatt attactatgt tcttaccatt gctggtcagt
    12001 ctaatgctat ggcgtatggc gaaggactgc cattaccggc cagggaagat gcgcctcatc
    12061 ccagaattaa acaattagcg agatttgcgc atacgcatcc cggaggcccg tcatgtcact
    12121 ttaacgacat tattccacta actcactgcc cacacgatgt tcaggatatg cagggttatc
    12181 accatcctct ggcaacgaat catcaaacac agtacggcac cgttggccag gcactgcata
    12241 ttgcacggaa attactgtcc tttattcctg ataacgcagg ggttctcatc gttccgtgtt
    12301 gccgtggcgg atcggctttt accgcgggca gcgaagggac atattcagaa cggcacggag
    12361 ccagccatga tgcttgtcgt tggggaacgg atactccgct ataccaggat ttagtcagca
    12421 gaacgcgagc cgcactggca aaaaatccgc agaacaaatt cctcggcgta tgctggatgc
    12481 aaggcgaatt tgacttaatg accagtgact acgcgtcaca ccctcaacac tttaatcata
    12541 tggttgaagc ctttcgtagg gatctaaaac aataccattc tcagcttaat aatattactg
    12601 acgcgccgtg gttttgcggc gataccacct ggtactggaa agaaaatttc cctcatgcgt
    12661 atgaagctat ttatggcaat tatcaaaata atgttttagc taatattatt ttcgtcgact
    12721 tccagcaaca aggtgaaaga ggactgacga acgcgcctga tgaagacccg gacgatttaa
    12781 tcatgggata ttacggttca gcgtaccgct caccggagaa ctggacgacg gcactgcgaa
    12841 gcagtcattt cagcgcggca gcccgtcggg ggattatttc tgacaggttt gtagaagcaa
    12901 ttttgcagtt ttggcgcgaa aagtgagcgt tcattaattt gatttattcg catccatcaa
    12961 cgcttttaac tcctccggcg taaacggtgg tttgcgtcgg tgcaacatcg catggcaatt
    13021 agggcacaca agcagcaaaa aaacaaatga gatcaaggta atatatcttc acttgcaggc
    13081 ttgaacagca gaaaatttgc gagcatgatc gcaattattt cactgctagg gatatcaatg
    13141 gcttccagta aatcgctaca gcaggcaatt gccaatataa aaatctggca taagggtgaa
    13201 cagcgcgcac cgcataaacc attgttattg ctatacgtat tagcgggata cctgaatgga
    13261 catccacgcc ttttcgatta tggatcggaa atctacgaac ctctgcacag tttactggaa
    13321 cgttttgggc cacagcgttc acagtaccga cctgatatgc cgttctggcg attacaaggc
    13381 gacggattct ggcaattaca caatgccgag ctttgctcaa cagcagggag cagccgacaa
    13441 ccgccagtga aggaactgaa cgaataccac gttgcaggcg gtttcgatga acagcactac
    13501 gccctggtaa ccggcaataa aaaactcatc aatactctag cgcaacagat actcgaagcg
    13561 cacttcaccg agagtattca ggaagaaatt gctgacgagc tgggttttga tctccagcaa
    13621 atccgtaaac agcgcgaccc gttatttcgc aaaaatgtat tacgcgctta taactaccag
    13681 tgcgcgatat gtggctttaa tatgcgccac gacgacacca ccgtcgcgct ggaagcggcg
    13741 catataaaat ggaaacagca cggcggtcct tgtgaaattc ctaatggcct ggcgctttgc
    13801 gcaattcacc ataaagcctt tgataaagga tcgattggcc tggacgaaaa tatgcgggta
    13861 ctggtatcag atgcagtaaa cggcggaggg agcgttgaga gattattctg ggattttgac
    13921 gggaaaatta ttgcgttgcc gcaggtgcgt aaaaattacc cttatgaggg gtttgtggag
    13981 tggcatcgga atgaggtttt tagggggtga ggttgataac tatcgcagta tgttcaatat
    14041 tgctgacata atcagtgcag gatcttgccg ctagtttaca actcgccttc gatagcggcg
    14101 caaaacgagt tctgttgccg atatcctggg ctatggatat tccaacagtt ccggcagagt
    14161 tgtttaccaa gtttcaggta agtttttact cagaaccggt tgatgcggtt tataaggcgc
    14221 tggatgtgaa ttaacacatt cctcagctta tttacaaaag gattattccc atttgatata
    14281 atatcagtgc caatttactt ttttcaatgg aatatatctc ggctaaataa caataaaact
    14341 tatcgctaat ggattaaata aacaatttt
//
LOCUS       DAJTOR010000099        14165 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTOR010000099 DAJTOR010000000
VERSION     DAJTOR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.099"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1987
                     /gene="yicI"
                     /locus_tag="OO249_002641"
     CDS             <1..1987
                     /gene="yicI"
                     /locus_tag="OO249_002641"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5636890.1"
                     /translation="FKSGNLSARVSKGEFWSLDFLRNGERITGQDTNNQRNYMFERLD
                     LGVGETVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVDHP
                     QSVSFEVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGL
                     WLTTSFTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPE
                     GMIRRLKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIY
                     DFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAHIYN
                     ELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLS
                     GFGFWSHYIGGFENTAPAHVYKCWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRF
                     FTQLKCRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPV
                     FTEAGDVQFYLPEGRWTHLWHNDELDGRCWHKQQHSFLSLPIYVRDNTLLALGNNDQR
                     PDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWT
                     LCLRNVVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(2040..3749)
                     /locus_tag="OO249_002642"
     CDS             complement(2040..3749)
                     /locus_tag="OO249_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5636891.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDAHLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(3870..5261)
                     /gene="xanP"
                     /locus_tag="OO249_002643"
     CDS             complement(3870..5261)
                     /gene="xanP"
                     /locus_tag="OO249_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5636892.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKTWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            5541..6746
                     /gene="gltS"
                     /locus_tag="OO249_002644"
     CDS             5541..6746
                     /gene="gltS"
                     /locus_tag="OO249_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5636893.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(6786..8867)
                     /gene="recG"
                     /locus_tag="OO249_002645"
     CDS             complement(6786..8867)
                     /gene="recG"
                     /locus_tag="OO249_002645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5636894.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(8873..9562)
                     /gene="trmH"
                     /locus_tag="OO249_002646"
     CDS             complement(8873..9562)
                     /gene="trmH"
                     /locus_tag="OO249_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5636895.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(9569..11677)
                     /gene="spoT"
                     /locus_tag="OO249_002647"
     CDS             complement(9569..11677)
                     /gene="spoT"
                     /locus_tag="OO249_002647"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5636896.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(11696..11971)
                     /gene="rpoZ"
                     /locus_tag="OO249_002648"
     CDS             complement(11696..11971)
                     /gene="rpoZ"
                     /locus_tag="OO249_002648"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5636897.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(12026..12649)
                     /gene="gmk"
                     /locus_tag="OO249_002649"
     CDS             complement(12026..12649)
                     /gene="gmk"
                     /locus_tag="OO249_002649"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5636898.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     DEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            12907..>14165
                     /gene="ligB"
                     /locus_tag="OO249_002650"
     CDS             12907..>14165
                     /gene="ligB"
                     /locus_tag="OO249_002650"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5636899.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRQQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDEMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     SVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVSL"
BASE COUNT         3170 a         3671 c         3904 g         3420 t
ORIGIN      
        1 gtttaaaagt ggcaacttaa gcgcgcgtgt cagcaaaggt gagttctggt cactcgattt
       61 tctgcgcaac ggtgaacgta ttaccggtca ggacacgaat aatcaacgaa attatatgtt
      121 tgagcggctt gatcttggcg ttggcgaaac agtttatggt ctgggagagc gttttactgc
      181 cctggtgcgc aatggtcaga cggtagagac ctggaaccgg gacggcggca caagtactga
      241 acaggcgtat aaaaatatcc cgttctatat gactaaccgt ggctatgggg tgctggtcga
      301 tcacccccag agtgtctctt ttgaagtggg atcggagaaa gtctccaaag tgcagttcag
      361 cgttgagagc gaatatctcg aatactttgt tatcgacggt ccgacgccga aagcggtact
      421 tgatcgttat acccgtttta ctggtcgtcc ggcgctgccg cccgcatggt ccttcggcct
      481 gtggctaacc acttcattta ccaccaacta cgacgaagcg acggtaaaca gctttatcga
      541 tggtatggcg gaacgcaatc tgccgctgca tgttttccac tttgactgtt tctggatgaa
      601 agccttccag tggtgcgatt ttgagtggga cccgctgact ttcccggacc cggaagggat
      661 gatccgccgc ctgaaagcga aagggctgaa aatctgcgtc tggattaacc cctatatcgg
      721 tcaaaaatcc cccgtcttta aagagttaca agagaaaggc tatttactca aacgcccgga
      781 cggttcgcta tggcagtggg ataaatggca gccaggtctg gcgatttatg actttaccaa
      841 tccggatgcc tgcaaatggt acgccgacaa actgaaaggt ctggtcgcga tgggcgttga
      901 ttgctttaag accgactttg gcgaacgtat cccaactgat gttcagtggt ttgacggttc
      961 cgatccgcag aaaatgcata accattatgc gcacatctac aacgaactgg tgtggaacgt
     1021 gctcaaggac accgttggtg aggaagaagc tgtcttgttt gcccgctcgg cctccgtcgg
     1081 tgcgcagaaa ttcccggtac actggggtgg cgattgttac gctaactacg aatcaatggc
     1141 ggaaagcctg cgcggtggtt tgtctattgg cctttcaggt tttggcttct ggagccacta
     1201 tatcggcggc tttgaaaata ccgctccggc gcacgtttac aaatgctggt gcgcgtttgg
     1261 tttgctctcc agccatagcc gtttacacgg tagcaaatct tatcgtgtgc cgtgggccta
     1321 cgatgatgag tcctgtgatg tggtgcgctt cttcacgcaa ctgaaatgcc gcatgatgcc
     1381 gtatctgtat cgtgaagctg cgcgtgcaaa cgcgcggggt acgccgatga tgcgggccat
     1441 gatgatggag ttcccggacg atccggcttg tgattacctt gaccgtcaat acatgttagg
     1501 cgacaacgtg atggttgcgc cggtgttcac tgaagcgggc gatgtgcagt tctacctgcc
     1561 ggaaggtcgc tggacacacc tgtggcacaa cgatgagctc gacggtcgtt gctggcataa
     1621 acagcagcac agcttcctga gtctgcccat ttatgtgcgt gataacaccc tactggcgct
     1681 gggcaacaac gatcaacgtc ccgattacgc gtggcacgaa ggcacggcat tccacctctt
     1741 taatctgcaa gacgggcatg aagccgtctg tgaagtgccc gctgctgacg gatcggtgat
     1801 ctttacttta aaagcagcac gtactggcaa cacgattact gtgactggtg cgggcgaggc
     1861 gaagaactgg acactgtgcc tgcgcaatgt tgtgaaagta aatggtctgc aagacggttc
     1921 gcaggctgaa agtgagcagg ggctggtggt gaagcctcaa gggaatgcgc tgacaattac
     1981 gttgtaatct attgatggaa tgccgggtac gtctgagtcg tacccggcga gattgatgtt
     2041 tactgcacca gctctgccga cgtaacttca ccgctggaaa cgatgccagc attcatggtt
     2101 tgagtcactt gctgctttgc ggcgttcacg gcatgcaatt gcccgctaac cgtaggtttc
     2161 agcggggcat tggcctgaat atctccactg gcggtaagct gaatattgcc gtcgccagtc
     2221 aacggtaatt caggccatcc ccattgttgc aaaatattca ccggcacacc gcgtccattc
     2281 aggctgatat gggtctgacg ttgtggcgtt tgtgaaacac tggcagtggc ttccagaatg
     2341 cctttttcag taaatgcact cagttcgctg atattcaccg tgctgctgtt ggcggtcagc
     2401 gccagcgacg ggcgacgaac atcaacacga ttgaatgtcg cggcggcggc attcagattc
     2461 gccgagccac tccagacgcc ccatttatga tcggtaacca gcgtcaggtt ggcaccgtaa
     2521 ccatcgagcg tggtgagctg ccacgggaag tcagggtcga tatcaatgat cagattgcgg
     2581 ctggcgctaa atctcttcag ttgcaggctg tttaaccaac cgggtgtcgt ttccatccac
     2641 aactgttgcc agtttttcgg caaggtatat tccagcccgg caattgccgc atcatcaagg
     2701 atcaacgttt tcccgtcacg cagccagttc cctgacgttc tgaccatacc cccttcccag
     2761 cggctggtga actggcgcag cgctacgccc tgcggggaaa attccgcgtt tataatcggg
     2821 tcaaataaat gcagcgaacc ataaatgaac tcgctagcgt tcatcgacag tttgccttct
     2881 tgtgtctgcc agtcatcttt actgaaggtc atgttgcgca agctgagatc gaggtcggtc
     2941 accgcccagt ccggaccttg caaatgagca tcgatcactt ccaggcgacc aatctgcaac
     3001 gacgggacag agcgtaatgg cgcaaagaag tcggttagcg atttttcgct ttgtagacgt
     3061 atatccgcca tgcgcaggtt ttccacttgc cagctgccgt cggcgttacg ctgcgcgttt
     3121 ccggttaatg tcccgcgggc gatgtcggca cccaggttag tcagcgtaac gcgatcgtta
     3181 tcaatactgc cttcaatcag tacattggtg gcaggaacat cattgagcga aagcgatccg
     3241 gcactaaact gaatctgcgc cttcgtaccc agcactttac cggcttttgg tgaccacgga
     3301 actacgccgc catttacccg ctgcgcgctc agtttccatt cgctattcgg gctattaaac
     3361 gccatatcac gcagttgcag acgatcggct ttgaacggta gcggcgcggt ctggtcggtg
     3421 agattcagcg tcccgttttc cagcaggatg gtatcgacat ggcgtggttc ggtcagttgc
     3481 cgactgctta gcgcaatgtc gacacttttg gccaccaggg tcgcgggctg accatcacga
     3541 ccaaacgtga cattctccag cacgatatga gatggcgcgg aaaaacggtg atccatcgcc
     3601 ccgaaggcca gatgatagtc gctattctcg gaaacccatg cgctgatatg ttctgctccc
     3661 cagcgggttt gcagaagaaa atagaggcca gcgatcacca ctaacagagc gatgagaatg
     3721 tagagaagca gcttcccaat aaatttcatg gtcttccatt ccgtgaaatg cacataagag
     3781 agttatgcac gatttacgcg caatcctcaa ggcgggaatg gtgaaagagg tgagactgcg
     3841 gcggtaatta tcttaccgcc gccagtgaat tactgttttt ctggtgggaa aatcagattc
     3901 agcacgatgg cagtaatacc gcccgcggcg atcccggagg agagcaggtt tttcagccat
     3961 tcaggggcaa actgcaaaat cagcggctgc tgagacacgc ccagaccaac cgccagcgac
     4021 agcgcgataa tcagaatcgc ccgacggttc agcggctcac gagaaacgat acgcacaccg
     4081 gaggcggcga tggtgccaaa cattacaagc gttgcgccgc ccagaaccgg ttctggaatg
     4141 tgttgtacaa aaccgctcac tgccgggaac agacccagca cgatcaacat cagcgcgacg
     4201 acaaaaccga catagcggct ggcaacacca gtcaactgga tcactccgtt gttttgcccg
     4261 aagcaggagt tcgggaaggt gttaaacacc gccgaaacaa acgagttcag gccgtttgcc
     4321 agcacgccgc ctttcaggcg tttcatgtac agcggaccgg acactggctg ttcggaaacg
     4381 tcagaggtcg acgtgatatc gccaatggtt tccagcgaag tgatcataaa gaccagcatc
     4441 agcggcagca gcagactcca ttcaatgcca agaccgtaat agagcggcgt tggcaccata
     4501 atcagttctt gcgtcatcgg ttcgttgctt tctggcaaca tgcccataaa ccacgccagc
     4561 gcatatccgg ccgccatcgc aatcaccagt gaggccacgc gtaagtaagg gttacgttga
     4621 cggttaagca ggataattaa ggctaagacc acgcctgcca gcagcagatt tttcggtgcg
     4681 ccgaaggtgt tatcgctcat ggctgcgtaa ccgccgccaa tggacgttaa cccaacctga
     4741 attagcgaca ggccgataat catcaccaca acgccagaaa ccagcggcgt aataatgcgg
     4801 cgcgccagat gcagaacgcg ggagatcacc atctcggtgc aacttgccag catcaacgtg
     4861 ccgaacaaag ccgccatcat ggtaggaaca tcagcaccac cggttttcag cgcggtaccg
     4921 cccataatca gcggggcaac aaagttgaag ctggtgccct gaatagacaa cagcccggag
     4981 ccaaccggac cccaggtctt aatttgaata atcgatgcca caccggaggc aaacagcgac
     5041 atactaataa tgtgttgcgt gtcttgtgcc ggtaaaccca gcgcctggca gattaatagc
     5101 gctggcgtga tcaccgcaac gaacatcgcc agcagatgct gacaggcggc aaacagggtt
     5161 tgaggaagcg gcggacgatc ttcaagacgg taaatcagtt cgctgttttg agtctgcgca
     5221 accggttgcg cattttctga ctcgagggtg gaaacagaca tcgaaaacaa tcccgtggta
     5281 gcaaagcggg cattttagct gaccgaaaga gaaaagcaaa cgtttgccac atcctcatca
     5341 aaatatttaa atgagataaa tgcacttttt tatataactt ttggtatttt tctgcttaaa
     5401 atccatgcca tttgttcatt aacagctgag aatttgaccc aggaaagcat aactcgatac
     5461 ccgtcatagt tcatgaagtt gccactgcgc gatgaagtat gacgagtatg aaagagtgat
     5521 gcggatacaa aggagtaact atgtttcatc tcgatacttt agcaacgctt gttgccgcaa
     5581 cgctgacgtt gctgctcggg cgtaagttgg tccattccgt ctcctttttg aagaaataca
     5641 ccataccgga acctgttgcg ggtggtttgt tggtggcgct ggcgctacta gtactgaaaa
     5701 aaagcatggg ctgggaagtc aactttgata tgtccctgcg cgatccgtta atgctggctt
     5761 tcttcgccac cattggcctg aacgccaaca ttgccagttt gcgtgccggt gggcgtgtgg
     5821 ttggcatctt cttgattgtg gttgttggtc tgttggtgat gcaaaatgcc attggcattg
     5881 gtatggctag cctgttaggg cttgatccgc tgatggggct gttggccggt tctattacgc
     5941 tttccggcgg tcacggtacg ggcgctgcgt ggagtaaatt gttcattgaa cgttatggct
     6001 tcaccaatgc gacggaagtg gcgatggcct gtgcaacgtt cggtctggtg ctgggcggct
     6061 tgattggcgg tccggtggcg cgctatctgg tgaaacactc caccacgccg aacggtattc
     6121 cggatgacca ggaagtcccg acggcgtttg aaaagccgga tgtgggacgc atgatcacct
     6181 cgttggtgct gattgaaact atcgcgctga ttgctatctg cctgacggtg gggaaaattg
     6241 ttgcgcaact tttggctggc actgcttttg aactgccgac cttcgtctgt gtactgtttg
     6301 ttggcgtgat tctgagcaac ggtctgtcaa taatgggctt ttaccgcgtc tttgagcgtg
     6361 cggtatccgt gctgggtaac gtaagcttgt cgttgttcct ggcgatggcg ttgatggggc
     6421 tgaaactgtg ggagctggct tcgctggcgc tgccgatgct ggcgattctg gtggtacaga
     6481 ccatcttcat ggcgttgtat gccatcttcg ttacttggcg catgatgggc aaaaactacg
     6541 atgcggcagt gctggctgcg ggtcactgtg gttttggcct cggtgcaacg ccaacggcaa
     6601 tcgccaacat gcaggcgatc actgaacgct ttggcccgtc gcacatggcg tttttggtgg
     6661 tgccgatggt cggtgcgttc tttatcgata tcgtcaatgc gctggtaatt aagttgtatt
     6721 tgatgttgcc gatttttgcc ggttaaccga tgaagcggaa gcggcggtct tctcaccgcc
     6781 gccttttacg cattcgagta acgttctgtc tccggcatcc agcgttctat caaggctttt
     6841 gcctgttgtg ggtaacgttc gtgaatatgt cgcgccaggc gctgaacttc cgggatcatc
     6901 gcctgatcgc gcagtaaatc cgccacttta aattcagcat tacccgtctg acgcgtgcct
     6961 aacaattcgc cagggccgcg aatctccaga tctttttgcg caatcacaaa accgtcgtta
     7021 ctgtcgcgca gcacttgcag gcgaatttgc gccgttttag aaagcggcgt tttgtagagc
     7081 agcacgcagt gagaagccac cgcgccacga cctacgcgcc cgcgcagctg gtgtaactgc
     7141 gccagaccca gacgctcagg gttttcgata atcatcaggc tggcgttagg cacatcaaca
     7201 ccgacttcaa taacggtagt ggcaaccagc agatgtagct caccttgttt aaacgacgcc
     7261 atcaccgcct gtttctcggc tggtttcatc cgcccgtgta ccaggccaac attcaattct
     7321 ggtagcgcca gtttcaactc ttcccaggta gcttccgccg cctgcgcttc cagtaactct
     7381 gactcttcaa tcaacgtaca aacccagtac gcctgacgac cttcagttat gcaggcatgg
     7441 cgcacgcggt caatgatgtc ggtacggcgg gtatcaggaa tagcgaccgt agtcactggc
     7501 gtgcggcctg gcggcagctc atctatcacc gaggtatcga gatcggcata cgccgtcatt
     7561 gccagcgtgc gggggatcgg cgtggcggtc atgatcaact gatgcggatg gaagccttgc
     7621 tgctggcctt tctcccataa cgccagacgc tgatgcacgc caaagcgatg ttgttcgtcg
     7681 ataatcacca gcgccagacc gttaaactgc acctgttcct ggaagatggc gtgcgtacca
     7741 acaatcatct gcacctgacc gctggcgatg gcttcctgct gagacagtcg cgctttgcct
     7801 ttctgcttac cggcgagcca gcccacttcg ataccgagcg gttcaaacca gttgcggaag
     7861 ttattggcgt gctgttcggc gagtaattcg gttggtgcca tcaatgccac ctgtttgccg
     7921 tgggcaatcg cacgcagcgc ggcgagggcg gcgaccagcg ttttaccgga acctacatcg
     7981 ccctgtacca ggcgcatcat cggcacatcc agcgccatat cgcgctcgat ctccgccact
     8041 acgcgtgcct gtgcgcccgt tggcttgaac ggtaaggctg cgaggagttt atttttcagc
     8101 gcatcattgg cgctcagcgg ctgggcatga aaacgctgtg ctccggcacg taaggctaac
     8161 atgctgaggt tgtgcgccag cagttcttcc agaatcagac gacgttgcgc cggatgctgc
     8221 ccggtttcca gatcgctaag ctgtagcgtc ggtggcgggc ggtgcaaagt gcgcaacgct
     8281 tccggtagcg tcatcattcc ttgtgacagt tccggcggca ggagttcttc aatggcgcag
     8341 gtgtcgagca gatccagcgc ctggtcggtt aatttacgca gcgtggcctg ctttacgcct
     8401 tccgttgttg gataaaccgg cgtgagcgtt tcctgtaatt ctggcgtgct gagatcgccc
     8461 tgcacgcggt attccgggtg gatcatctcc gcaccatatt taccgcgctt tgcttcgcca
     8521 taagccagta cacggcggcc cgtcgccagg ctatttttca ttgccgcgct gaaattgaaa
     8581 aagcgcatgg tgaggatgcc ggaaccgtcg ctgatctggc aggtcatcat ccgccgaccg
     8641 ccgaaggaga tattgcagtt cagcacttcg ccttccaccg tggcataaac gcccggcagt
     8701 agttctccga tggggtagag atgggtgcga tcttcgtagc gcaggggaag gtgtaagagt
     8761 agatcctgca cggtatgcag gttgattttc gccagtttgt tactaagtgc tgcgccaacg
     8821 cccgttaggg aactgagtgg gacagcatct aacaggcgac ctgtcatggc acttaccctg
     8881 cagcctgcat agtagcccac cagtcggcat cagcttcgat ctcgccttgc tgattgacgt
     8941 ggggataagg caggcctttg cgttttgcga ctttcgccag caccggatag ccgccttcaa
     9001 acaacaggcg ttgttgctct gcttccggca acatgctgtt ttcacgcagg tacatgcctg
     9061 cattttgccg ctgacgctgg gcttcgtaaa gaatgagggc tgaggcaacg gaaacattca
     9121 gcgactgcac catgccgatc atcggaatga tgatgtcctg atccgccagg gccaatgctt
     9181 cctgcgtgat gcccgttttc tcctgtccca tcaaaatgca ggtcgggcga gtgtaatcaa
     9241 tttcgcggaa atcgacagcg ttatcagaaa gatgggttgc cagaatctgc atgccctggc
     9301 ctttgagatg agcgacggca tcgccaatgg tgcggtgtgt tttcacctgt acccagctgt
     9361 tactacccgc cgctgccgaa gccatggtgc gcatgcggct accaggccag acggcgtgaa
     9421 cttcatgtac gccaacggca tctgcggtac gaataatcgc agaaacgtta tgaggtttgt
     9481 ggacctgctc catgcagacg gtcagatcag gctgccgcct ggcgagcatt tcgcagatgc
     9541 gtgcataacg tgttgggttc ataaaacatt aatttcggtt tcgggtgact ttaatcacgt
     9601 ctggcatcac gcggattttg cgcatgatat tcgccagatg cacacggtca cgagcggtca
     9661 gacgaataaa ggcgctgtag acgcgaccat ctttctcttc cgtattcaaa ctttgaatat
     9721 tcgaagtcgt ggtgttaatt gccgccgtca ggtttgccag cgcaccctga tgattgaaca
     9781 tctccacctt gatttcggtg atgaactcct gcgccgtctc tttatcccat tccacagcca
     9841 taaacttctc tggctctttc tggtagccac ggatgttacg gcaggattca tggtggatca
     9901 ccagaccttt accggggctg acgtgggcga taatcgggtc gccaggaata gggcggcagc
     9961 atttcgcaaa ggtgatcagc acgccatccg cgcctttgat cggcagatgc ccgtggcttt
    10021 gagtcgccgg tggaatggag gcgtccccat gttgtagatt tttcgcgacc accacgctca
    10081 ttgcgttacc aaggccgatt tctgccagca gatcgtcaag cgttgccagc ttcatgcgat
    10141 ccagctcgcg ctgaatattt tcctgcggga tttcattgag cttacggcta ccacccaaag
    10201 catggttgag cagacgacgg cccaggctta cagaatcatc acgcttgagg tttttcagca
    10261 actgacgaat tttggcgcgc gctttcgagc taacgacaaa gttcagccaa gcggcattcg
    10321 ggcgagcgcc cggagcggta atgatttcaa cggtctgacc gctagtaagc ggctgcgaca
    10381 gcgggtaagg ctggcggtcg acgcgtgcgc ccacgcaggc atggccgata tcggtatgca
    10441 ctgcataagc gaagtcgacg ggcgttgcac cggcaggcag ctcgacaatg cgcccttccg
    10501 gtgtgaaaac gtaaatctca tccgggaaga gatcggattt aacgctctcg ataaattcaa
    10561 acgaactacc ggcgctctgt tgcagctcca gcaggctttg catccagcgc tgggcgcgta
    10621 tttgtgcggt agtactggtt tcgccgtgct ctttataagc ccagtgcgcg gcaacaccca
    10681 tctccgccat ctgatccata tcttcggtac ggatctggac ctcaaccggc acgccgtgcg
    10741 ggccaatcat cgaggtgtgc aacgactgat agccgttcgc ttttggaatg gcgatatagt
    10801 ctttcacgcg gcccggacgc ggcttgtaca ggctgtgcat ctggcccagc acgcgataac
    10861 aggtgtcaga atcattgacg atcacgcgga aagcgtagat gtccatgatt gagtgaaaac
    10921 gctgctcttt gagcaccatt ttgcagtaaa tcgaataaag atgcttttcg cgaccactga
    10981 cgcggcacgg tattcccgct tcctgcaaac gcccttcgat ttcagaaaga atcttctgga
    11041 tcatctcttt acggttgccg cgcgcggctt tcaccacttc tttgattacg cgataacggt
    11101 tgggatacag cgcctcaaaa cccagctctt cgagttcggt tttaatgtgg tggataccta
    11161 aacggtgcgc cagcgggctg taaatttcga gagtttcacg ggcgatgcgg cgacgtttgt
    11221 ccgggcgaag tgagcccagc gtgcgcatgt tgtgggtacg gtcggcaagt ttgatgagga
    11281 tgacgcggat atcctgcacc atcgccataa tcatcttgcg aaagttttcg gcctgcgcct
    11341 ctttcttatc gcggaacttg agtttatcaa gtttcgacac cccctctacc agctcggcga
    11401 cgcttttacc aaaaagctgt tccatatcct ggtaggtggc gggagtatct tcaatcacgt
    11461 catgcagcag cgccgccatc agcgtttcat agtcgagttt catctcggcc agaatgcagg
    11521 caaccgctac cgggtgcgtg atatagggtt caccgcttga acgtgtttgc ccctcgtgag
    11581 catcacgtgc aacgagatac gcctgccgca gacgcttgat ttggtcttcc ggcaggtagg
    11641 tttgaatcag ttgattcagg ctttcaaaca gatacaaggg cgacccgctt tgtgattaac
    11701 gacgaccttc agcaatagcg gtaacggctt gtaattcagc ggcttcctgc tcttgctgtt
    11761 cctggcgttc gcgaacgtcg aggatctggt tgttgatcag accttcttcg atttcgcgca
    11821 gcgcgattac agtggtttta tcgttttctt ccggtaccag cggatccttt ccgcctacct
    11881 gcatctgacg agcgcgacgc gcggcgacca gtaccaggtc aaaacggtta ccaattttct
    11941 ctacagcgtc ctgaacagtt acgcgtgcca tacttaaaaa gctccacagg tgaagaaatg
    12001 actgggcatg atactgaaat caggttcagt ctgccaacaa tttgctgatt aaagcgtcat
    12061 gacgctgctt ttggcggctc atgcgcagac gttcggcgcg aataatggtc ttcaaatcgg
    12121 tcaacgcggt atcgaagtca tcattcacaa tcaggtaatc atattcgtcg taatggctca
    12181 tttctgcaac agcttgcgcc atacgctttg caatgacctc ttcgctgtcc tgaccgcgac
    12241 cgcgtagacg gcggtccagt tcaattttag acggcggtaa aataaagata ctccgcgcgt
    12301 gcggcatctt ctggcgaatt tgctgcgcgc cctgccagtc gatatcgaga aaaacatcga
    12361 caccggtcgc cagtacttgc tcaatggcct cacgcgaagt gccatagtaa ttaccaaaaa
    12421 cttctgcgtg ttcgaggaac gcatctctgc taatcatttc tttaaattca tcatgattaa
    12481 caaagaaata atgttcaccg tggacttcac caggacgcgg ttggcgtgtg gtgtgtgaaa
    12541 cagaaacctg ggtgtcatac aacggttggg tttttaataa agcctgaatc aggctggatt
    12601 tacccgcgcc actgggggca gaaacaatat aaagcgtgcc ttgagccatg agtatctttc
    12661 gtatgtgatt agcgaaataa agcctacata cgagcttatt atacacggcg cggccatgtg
    12721 acgtagccct tgctcacact ttttgcgcaa agttgttgcg ttttgcgtag ctgcttccgc
    12781 atttgcctgt gatttactgc aataaagacg attctcagga agcgtcctcg cagactccgt
    12841 ttttgccact cggctttgcc ttcaccacgt tgtatatctg ccagcctgac tacagcggag
    12901 gtgaagatga aagtatggat ggcgatatta ataagtatct tgtgctggca atcatctgtg
    12961 tgggcggtct gtccggcctg gtcgccagcc agagcacagg aagaaatttt ccgccagcaa
    13021 cagcaaataa aacagtggga cgatgactac tggaaggaag gaaaaagtga ggtggaagac
    13081 ggtgtttacg atcagttaag cgcccgtctt acgcagtggc aacgctgctt tgtgagcgag
    13141 ccccgcgacg agatgatgcc gcctttaaat ggtgcggtta tgcatccggt tgcccatacg
    13201 ggtgtacgca aaatggcgga taaaaacgca ttaagtctgt ggatgcgaga acgtagcgat
    13261 ctttgggtgc agccaaaagt tgatggcgtt gcggtaaccc tggtttatcg ggacgggaaa
    13321 ctgaacaaag caatcagtcg cggtaatggc ctgaaaggcg aggactggac gcagaaagtt
    13381 agcttaattt ccgctgtgcc gcaaaccgtt agcgggcctt tagccaacag tacgcttcag
    13441 ggggaaatat ttctccagcg cgaggggcat atccaacaac aaatgggggg aataaatgcc
    13501 cgcgcaaaag ttgctggctt gatgatgcgc caggacgata gcgacacgct gaattctctg
    13561 ggcgtttttg tctgggcatg gccggatgga ccgcagttaa tgtctgatcg tttaaaagag
    13621 ctggctaccg caggttttac tctaacgcag acgtataccc gtgcggtgaa aaatgctgat
    13681 gaagttgcgc gcgtacgcaa tgagtggtgg aaagcggaat tacccttcgt caccgatggc
    13741 gtagttgtac gagcggcgaa agagccagaa tcccgccatt ggctaccggg ccaggcagag
    13801 tggctggtgg cctggaaata tcaacctgta gctcaggttg ccgaagtgaa ggcgattcag
    13861 tttgcggtgg gtaagagcgg taaaatatcg gtggttgcgt cactcgcatc tgtcatgctg
    13921 gatgataaaa aagtccagcg ggtgaatatt ggttccgtca ggcgctggca ggagtgggat
    13981 attgcgcctg gtgatcagat tctcgtcagc cttgccggtc agggtattcc tcgcattgat
    14041 gatgtggtgt ggcgcggtgc agaacgtaca aaaccgacac cgccagaaaa ccgctttaac
    14101 tcgttgacct gctactttgc ttctgatgtt tgtcaggaac agttcatttc acgcttagtc
    14161 agtct
//
LOCUS       DAJTOR010000100        14051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTOR010000100 DAJTOR010000000
VERSION     DAJTOR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.100"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            278..637
                     /locus_tag="OO249_002651"
     CDS             278..637
                     /locus_tag="OO249_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5636900.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            639..1031
                     /locus_tag="OO249_002652"
     CDS             639..1031
                     /locus_tag="OO249_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5636901.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGVRAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(1082..2497)
                     /gene="gltX"
                     /locus_tag="OO249_002653"
     CDS             complement(1082..2497)
                     /gene="gltX"
                     /locus_tag="OO249_002653"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5636902.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            2758..2833
                     /locus_tag="OO249_002654"
     tRNA            2758..2833
                     /locus_tag="OO249_002654"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2791..2793,aa:Val,seq:tac)
     gene            2878..2953
                     /locus_tag="OO249_002655"
     tRNA            2878..2953
                     /locus_tag="OO249_002655"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2911..2913,aa:Val,seq:tac)
     gene            3000..3075
                     /locus_tag="OO249_002656"
     tRNA            3000..3075
                     /locus_tag="OO249_002656"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3033..3035,aa:Val,seq:tac)
     gene            3080..3155
                     /locus_tag="OO249_002657"
     tRNA            3080..3155
                     /locus_tag="OO249_002657"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3113..3115,aa:Lys,seq:ttt)
     gene            3275..3616
                     /locus_tag="OO249_002658"
     CDS             3275..3616
                     /locus_tag="OO249_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5636903.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLDVSLLNP
                     VEKTTHIDIYI"
     gene            complement(3607..4533)
                     /locus_tag="OO249_002659"
     CDS             complement(3607..4533)
                     /locus_tag="OO249_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5636904.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            4623..5621
                     /locus_tag="OO249_002660"
     CDS             4623..5621
                     /locus_tag="OO249_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5636905.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(5618..5836)
                     /locus_tag="OO249_002661"
     CDS             complement(5618..5836)
                     /locus_tag="OO249_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5636906.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(5838..7853)
                     /gene="ligA"
                     /locus_tag="OO249_002662"
     CDS             complement(5838..7853)
                     /gene="ligA"
                     /locus_tag="OO249_002662"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5636907.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIVGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(7924..8910)
                     /gene="zipA"
                     /locus_tag="OO249_002663"
     CDS             complement(7924..8910)
                     /gene="zipA"
                     /locus_tag="OO249_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5636908.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            9140..9901
                     /gene="cysZ"
                     /locus_tag="OO249_002664"
     CDS             9140..9901
                     /gene="cysZ"
                     /locus_tag="OO249_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5636909.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            10086..11057
                     /gene="cysK"
                     /locus_tag="OO249_002665"
     CDS             10086..11057
                     /gene="cysK"
                     /locus_tag="OO249_002665"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5636910.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11441..11698
                     /gene="ptsH"
                     /locus_tag="OO249_002666"
     CDS             11441..11698
                     /gene="ptsH"
                     /locus_tag="OO249_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5636911.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11743..13470
                     /gene="ptsI"
                     /locus_tag="OO249_002667"
     CDS             11743..13470
                     /gene="ptsI"
                     /locus_tag="OO249_002667"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5636912.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13511..14020
                     /gene="crr"
                     /locus_tag="OO249_002668"
     CDS             13511..14020
                     /gene="crr"
                     /locus_tag="OO249_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5636913.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLPGSVTVG
                     ETPVIRIKK"
BASE COUNT         3462 a         3613 c         3476 g         3500 t
ORIGIN      
        1 cgaaccgctg acctcttgca tgccatgcaa gcgctctccc agctgagcta tagccccgtc
       61 acgtaaagct tgtcgagttg acgggcggca tcatatgaat tccgcccgaa tgtgtcaacg
      121 gcaaattgca acgtgtagtt tcaatcgctg aaaaatcagg caaatgaaca attttggaaa
      181 accgctcgca ttcagtagtt attcatgtca cggtttcctg taaagtggtg ttataaaatg
      241 aactactaat agacccacat acattcaggg aattgttatg ttcaaggaga ggatgacacc
      301 agatgaactt gccagactga ccggctatag ccgccagacc attaataaat gggtacgcaa
      361 ggaaggctgg acaacgtcac caaaacctgg cgtccagggt ggcaaagccc gactggttca
      421 cgtcaatgaa caggttcgtg aatatattcg caatgctgaa cgtccagaag gtcagggaga
      481 agcgcctgcc ctttctggtg atgcgccgct tgaagtattg ctggtgacac tggcgaaaga
      541 gatgacgcca gttgaacaaa aacagtttac atccttgctt ctgcgggaag ggattatcgg
      601 attgttacaa cgcttaggga ttcgcgatag caaataatat gaaaagatta cgcaataaaa
      661 tgaccaccga agaactggct gaatgcctcg gtgtggctaa acaaactgtt aaccgttgga
      721 tcagagaaaa aggctggaaa acggaaaaat ttcccggcgt gaaaggcggt cgtgccagac
      781 ttattctggt cgatacgcaa gtttgcgagt ttattcagaa cacgccagcc ttccataaca
      841 caccaatgtt gatggaagct gaagagagaa ttgctgaata cgctcctgga gtacgcgccc
      901 cggcgtatcg acaaatcatc aacgcgatag ataatatgac agacattgag caggaaaaag
      961 tcgcgcaatt tttgtcacgt gaaggaattc gcaatttcct cgcccgtctc gatattgacg
     1021 aatcagcata aataaaaaac ggcaggatat tatctcctgc cgtttatctt ttatgcgcta
     1081 attactgctg gttttcgcgt tcagcaataa aagccagcgc tttgttgata cgctcaatac
     1141 tgcgggtcct accaattgcg tgaacggtaa catccagtgc cggagactgc cccgcaccgg
     1201 ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc tcatccgccg
     1261 tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg gccagtttgt
     1321 cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc gcggcgtcgg
     1381 catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc tctttcagcg
     1441 tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg ttacgggtat
     1501 cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc ggcggcagcg
     1561 cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca ctggcagatt
     1621 tgctgacggc attcagggtg aagtatttga tcatctcttc acgagtgaag atttcctgat
     1681 cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc aaataaccgt
     1741 catcacgata ctgcattacg ctgaccgccc cgtgacgttt ggacagtttt ttaccgtcat
     1801 cgccgttgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg gccttaagaa
     1861 tgttgatctg gcgtggcgtg ttgttgatat ggtcttcgcc acggataacg tgggtgattt
     1921 ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca tcggtacggc
     1981 gaataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg atctgatcgt
     2041 caaaaacaac agaaccttcc tgcgggttag caaaacgtac aacgcacggt tcatcatcag
     2101 cgtgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca cctttcgcca
     2161 tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta taagcagtgc
     2221 cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg gtctggtagt
     2281 acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg gcttcgatag
     2341 cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac tcaccgccgt
     2401 ggttacgtgc aaaaagccag gagtaaaggg cagtacgcgc gccgccaacg tgcagatagc
     2461 ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac gtttagaaag
     2521 atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc actcccgcct
     2581 gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt gacaaattgc
     2641 gcaacacggt ctattttgcg atcatcttgc ttaaatttac gacgaacgaa caatttcttt
     2701 agaaaatgcg ttgactcatt ttgaactctc cctataatgc gactccacac agcggggggg
     2761 tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg ttcgatcccg
     2821 tcatcaccca ccaactactt tatgtagtct ccgccgtgta gcaagaaatt gagaagtggg
     2881 tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg ttcgatcccg
     2941 tcatcaccca ccactttctc accagctaaa tttcttgtaa caatgtgaag taccgaagtg
     3001 ggtgattagc tcagctggga gagcacctcc cttacaagga gggggtcggc ggttcgatcc
     3061 cgtcatcacc caccacttcg ggtcgttagc tcagttggta gagcagttga cttttaatca
     3121 attggtcgca ggttcgaatc ctgcacgacc caccaatgta aaaaagcgcc ctaaaggcgc
     3181 ttttttgcta tctgcgattt gtaagattga tttttcttct caagttcctt gttcttctac
     3241 cgatattacc tcgtacagtg acgaggactg aacaatggcc actatctctt caacttcaat
     3301 tccttccata caaacacaga gtagtaacag ggcatcacaa ggtagcgatg ttgcttcaca
     3361 aatcgcgcgg ataagtcagc aaatcatcaa actcactcaa caaattaaag agattgtaga
     3421 cactagcggc agtgctgaag acaagcaaaa acaggccgaa ttgattcaac agcaaatcac
     3481 cctactggaa acacaactgg cgcagttgca gaaacagcag gcagaaaagg cgcaggagaa
     3541 agagcagcgt ttgtctctgg atgtatccct tctaaatcct gttgaaaaaa cgactcatat
     3601 cgatatctat atctgataca acggatcccc ttcccgacca gccattagcg cattgccctg
     3661 gtctcgtacc acgtcccata acgcttcagc ggcggtggaa agtgaacggt tcttcctgcg
     3721 caccagcatc aactggcgtt caacaaccgg cgtaatgcgc tttaccacca gtgggctacc
     3781 ttcaggcaat ggtagcgcca gtgcgggaag aatactgatg ccgatgcctg cggctaccat
     3841 cgggaacagc gtcgccggat gtccaatctc ctgcacaata ttcgcctgaa taccattgcg
     3901 cgccagcgct gcgtcaatca gcggtcggct gcctgacgcg taatcctgca acaccaattt
     3961 cgctccctgt aatgcctgcc agggtacata atcttccacg gccaaagcgc tatcgcgatg
     4021 gcaaagcaga aagaaaggct cggaaagaat cgcttcacat tgcagatcgc ccactgggcc
     4081 tggatcgatg acgatgccaa agtcgacatc tccctggcga atactttcca tcacccactg
     4141 ctgcggacga tcgtgcagga caaactgaat atctggatag cggcgatggc tttcagcaat
     4201 gcattgcgga ataagatgcg cggaaatggt ctggctggca gcgacccgaa cttttccgct
     4261 cagttgttgc cccatacgcc cggtatcgcg caacgtgctg ttaagttcat ccagcagtcg
     4321 ctcaagacgc aaagccaact gctgccctgc atctgtaagc accacttcac gcgtggttct
     4381 gtccagcagg cgaacaccag tatgattttc cagctccttc acactgtgac ttactgccga
     4441 ctggctcaag ccgatacgct ctcctgcacg actaaaactt ttctcctgcg ctactgtgac
     4501 gaaaaccttt aattgtttta aagaataatt catctgtttt cttcatgaat ggatgcaata
     4561 aatcaatttt atttctcaaa ctggaagaag cacaatagaa ccatcgatca tctggagtct
     4621 ttatgaaact ttttcgtatc ctcgatcctt tcaccttaac cctgatcacg gtggtgttgc
     4681 tggcctcttt ctttccggcc agaggcgatt tcgtcccctt ctttgaaaat ctgaccaccg
     4741 cagctattgc cctgctgttc tttatgcacg gcgcgaagtt gtcgcgtgag gcgattattg
     4801 ctggcggtgg tcactggcga ctgcatttgt gggtaatgtg cagcaccttc gtgctgtttc
     4861 cgattctggg tgtactgttt gcctggtgga aaccggtaaa tgtcgacccg atgctctact
     4921 ccggttttct ctacttgtgc actctcccgg ctaccgtgca gtctgcaatc gccttcacgt
     4981 caatggcggg cggtaacgtc gcggcggcgg tttgttctgc gtcagcatcc agcctgctgg
     5041 gaattttcct ttcaccattg ctggttggtc tggtgatgaa tgttcacggt gcagggggca
     5101 gccttgagca ggtcggtaaa attatgctgc aactgctgct gccgtttgtg ttggggcatc
     5161 tttcccggcc gtggattggt gactgggtgt cgcgcaataa aaaatggatt gcgaaaactg
     5221 accagacgtc cattctgttg gtggtttata cagcgttcag cgaagccgtc gttaatggta
     5281 tctggcataa agttggctgg ggatcattgc tgtttatcgt ggtggtcagc tgcgttcttc
     5341 tggctatcgt gattgtagtt aacgtcttta tggcacgccg actgggcttc aataaggcag
     5401 atgaaattac tatcgtcttt tgtggttcga aaaagagtct ggcaaatggc atcccgatgg
     5461 caaacattct gttccccaca tcggtgatcg gtatgatggt gctgcccctg atgattttcc
     5521 atcagatcca attgatggtc tgtgcggtgc tggcgcgtcg atacaaacgc cagaccgaac
     5581 agttacaggc gcagcaggaa agcagcgccg ataaagctta aagcggacgc ttcaggggct
     5641 ggaccagttg cgtcagcccc tcggttttaa tcagcagcgt gatttgcatt agctcaccga
     5701 gcttccctgc cgggaactca tctttgcggg caaaccacag cagatactcc tccggcaggt
     5761 cgattaagcg acgccctttg tatttgccaa acggcattat cgtattggct atttcaatca
     5821 gctgctcttt ttccatctca gctacccagc aaacgcagca tttccgcttc gtcgatgact
     5881 tcaatgccca gttcctgcgc cttcgccagt ttagatcctg cagcttcacc cacaatcacc
     5941 agatcggttt tcttcgacac gctgcccgcg actttcgcgc ccagttcgac cagtcgagct
     6001 ttagcgtcat cacgcgacat ctggcttaag ctgcccgtaa gcaccacggt tttaccagca
     6061 aacgggctgt caatctcttc cgcgttgata acgatcggcg caggccagtg aacaccttcc
     6121 gccaacagct cgctgatgac attgcggttg ctttcttcgg caaagaagtt gtgaacgtgg
     6181 gatgcaacga caatgccaac atcaggcacc ttttgcagct cttcaatcga agcggcttcc
     6241 agcgcttcca gcgtgccgaa atatgccgcc agacctgctg cggtggcctc gccgacttca
     6301 cggatgccaa gtgcatagag gaagcgagca aaggtggttt ctttcgcttt ttccagcgcg
     6361 ttaaccacgt tttgtgccga ttttggcccc atacgctcca gtccggtcag tttgcctgcg
     6421 gtgagtttga acagatctgc cggagtgtgg acatattctt tttcaaccag ctgatcgatg
     6481 attttgtcgc ccattccgtc aacatccatc gcacggcggg aaacaaagtg tttcagcgac
     6541 tctttacgct gcgcaccgca aatcaggcca ccggtacagc gggcaaccgc ttcaccttcc
     6601 acacgctcaa cgtcagaacc acataccgga caatgcgtcg ggaatacaac ctcacgggta
     6661 tcttccgggc gttcagaaag cacgacgtta accacctgcg ggatcacgtc gccagcgcgg
     6721 cgaatcacca ctttatcgcc aatgcgtaaa ccaagacgtt cgatttcatc cgcattgtgt
     6781 aaggttgcgt tactcaccag cacgccagca acatggacag gttccagacg cgcaacaggc
     6841 gtaatcgcgc cagtacgccc aacctgaaac tcgacgtcac gcacaaaggt catctgctcc
     6901 tgcgccggga atttaaacgc taccgcccag cgcggggcac gcgcgacaaa gccaagctgc
     6961 tcctgctgtt ccagtgagtt gaccttaatc accacgccgt cgatatcaaa gcccagcgtc
     7021 gggcggtctt cttccacttt gtgatagaac gccagcactt cttccgccga ttcacaaagc
     7081 gttacccgat cgctgaccgg caacccccac tttttaaatt gcagtaaacg gccaagatga
     7141 gtatccggca gctcgccacc ttccagaaca ccaacgccat agcagaaaaa agtgagcggt
     7201 cgcttcgctg taatacgcgg atcaagctga cgcagtgaac cagctgccgc attacgtggg
     7261 ttagcaaaaa ctttcccgcc cgtgcgtcgc gcatcttcgt taatcttttc gaaccccgcc
     7321 tgcggcagga acacttcacc acgcacttcc agacgcgccg ggatattctc tccatgcagc
     7381 ttcagcggaa tggcgcgaat agtacgcaca ttagacgtga tatcttcccc ggtggtgcca
     7441 tcgccacggg tcgcggcact gactaaaacg ccattttcat acagaatact gacggcaagg
     7501 ccatccagct tcagctcaca gcaccaggtg actttctcgt tgtttttcag acggtcctgc
     7561 acacgtttgt tgaaagcaag aaagctttct tcatcaaaaa cgttatccag tgacagcatt
     7621 ggtacttcat ggcgtatctg gctgaaagcc gccagcggcg cagcacctac acgttgggta
     7681 ggcgaatcag gcgtaatcag ttctggatgt ttagtttcca gctcgcgcag ttcgcgcatc
     7741 agcctgtcgt attcagcgtc gggaatttcc ggcgcatcca tcacatgata aagatattca
     7801 tgatggcgaa gcgtcgttcg cagttctgtc agttgttgtt cgattgattc catatcgcac
     7861 catcaatgct aaaaaccccc gacaagcggg ggttcgaaga agagttaatt tgccttaagt
     7921 gtatcaggcg ttggcgtctt tgacttcgcg gatgatgtcc tggtactcgc gcaatttctg
     7981 cggagtcatc atacggcgct gatcgtcaag cacgacaccg cccacttcat cggcaatatg
     8041 ctgcgcagat tgcagcatca gcttgaagtt ctgcagctcg tcaccgtaag acggtacctg
     8101 cataaagata gtgacacccg gagtagtgaa atccttcatt tcaggatcaa aggttcccgg
     8161 tttcaccata ttcgccaggc tgaataacgc cgggccgctg ccatccgggc taagatgacg
     8221 atggtaaata ttcatatcgc caaaaatgaa gcccgcttgt tgaatgctgt taagaagcag
     8281 ttcaccgttt agctcgctac cgtgatgcgc cgcgacgttc atgataatca ccgcttcttt
     8341 gcgcttcggt ttatccataa ctggagccgg ttccgctaca ggctcaggct gtggtgccgc
     8401 tacgggttct gcaggctgga aagcctgttg tgccggttgc ggtgctgaat gcacaggctg
     8461 cggcgctggc gcgacctgtg gcgaaactgg ctgctgcaac ggctgttcag gctgcggctg
     8521 atagacaggc tgctgcaccg gctgcgctgg acgcggagca tgttgcggcg gtacctgcgc
     8581 ttcaggcggc tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gttggtactg
     8641 gtgttgcggc gacggacgag cagcctcatg ctcctgagcg ttagccgggg catgattcac
     8701 gcggtgaaca cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc
     8761 gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc
     8821 tttacggctg gtccagaaac catgtaccag taaagcgatt atggcgatcg cgccaacaat
     8881 gattaatatc agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca
     8941 ccacggcaaa catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta
     9001 ttaactttca cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat
     9061 cctaactgtc cattgcgcaa ttacccggta aaatacgcag aattttccta ggattggtca
     9121 aaaggagctc atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta
     9181 ctattttgcg caaggctgga agctcgtctc gcaacctggg attcggcgtt tcgttatttt
     9241 accgctgctg gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacacagct
     9301 cgatgtctgg atcccgactc tcatgagtta cgttccggac tggctacaat ggctgagtta
     9361 tctgttgtgg cctctggctg tcatctctgt gctgttagtg tttggctatt tcttctccac
     9421 gattgctaac tggattgccg ctccgtttaa cggtttattg gctgaacaac tggaagcacg
     9481 attgactggc gctacaccgc cagataccgg gattttcggt atcatgaaag atgtgccgcg
     9541 aatcatgaaa cgcgaatggc aaaaatttgc ctggtatctg ccgcgcgcaa ttgtattact
     9601 aattctttac ttcatccctg gtattgggca aaccgtcgcg ccggtactgt ggttcctgtt
     9661 tagcgcctgg atgttagcca tccagtattg cgattacccc ttcgataacc acaaagtgcc
     9721 gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc
     9781 tttaaccagc ctgtttacga tgatcccgct gcttaatctg ttcatcatgc ccgttgccgt
     9841 ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat aaacacgcga tgtggcggta
     9901 acaatctacc ggttattttg taaaccgttt gtgtgaaaca ggggtggctt atgccgcccc
     9961 ttattccatc ttgcatgtca ttatttccct tctgtatata gatatgctaa atccttactt
    10021 ccgcatattc tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac
    10081 aggccatgag taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc
    10141 gcctgaatcg catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct
    10201 tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc
    10261 tgaaaccagg cgttgaactg gttgaaccga ccagcggtaa taccgggatt gcactggcct
    10321 atgtagctgc cgctcgcggt tacaaactca ccctgaccat gccagaaacc atgagtattg
    10381 aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt gctgacggaa ggtgctaaag
    10441 gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc cagtaatcca gagaaatacc
    10501 tgctgctgca acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc
    10561 cggagatctg ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg
    10621 gcggtacgct gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta
    10681 tctctgtcgc cgttgagcca accgattctc cagttatcgc ccaggcgctg gcaggtgaag
    10741 agattaaacc tggcccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc
    10801 tcgatctcaa gctggtcgat aaagtcattg gcatcaccaa tgaagaagcc atttctaccg
    10861 cccgtcgtct aatggaagaa gaaggtattc ttgcaggtat ctcttctgga gcagctgttg
    10921 ccgcggcgtt gaaactacaa gaagatgaaa gctttaccaa caagaatatt gtggttattc
    10981 taccatcatc gggtgagcgt tatttaagca ccgcattgtt tgccgatctc ttcactgaga
    11041 aagaattgca acagtaatgc cagcttgtta aaaatgcgta aaaaagcacc tttttaggtg
    11101 cttttttgtg gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac
    11161 tggtatttaa ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa
    11221 agctgaatcg attttatgat ttggttcaat tcttccttta gcggcataat gtttaatgac
    11281 gtacgaaacg tcagcggtca acacccgcca gcaatggact gtattgcgct cttcgtgcgt
    11341 cgcgtctatt aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact
    11401 ttagttccac aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac
    11461 cattacagct ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa
    11521 gggcttcact tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct
    11581 gtttaaactg cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg
    11641 cgaagacgag caaaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt
    11701 tcccgggttc ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta
    11761 gcatccccgg gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt
    11821 gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt tctgagcggt
    11881 cgtgccaagg catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt
    11941 gaagaaaaag aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag
    12001 caggaaatca tagccctgat taaagataaa cacatgacag ctgacgcagc tgctcatgaa
    12061 gttatcgaag gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt
    12121 gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt
    12181 atcgacctga gcgccattca ggatgaagtc attctggttg ccgctgacct gacgccgtcc
    12241 gaaaccgcac agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt
    12301 acttcccaca cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt
    12361 agcgtcacct ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag
    12421 gtttacgtca atccaaccaa cgaagttatt gataaaatgc gtgctgttca ggagcaagtg
    12481 gcttctgaaa aagcagagct tgctaaactg aaagatctgc cagctattac gctggacggt
    12541 caccaggtag aagtatgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt
    12601 aacggcgctg aaggcgttgg tctgtatcgt actgagttcc tattcatgga ccgcgacgcg
    12661 ctgcccactg aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctcg
    12721 caggcggtta tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac
    12781 ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt
    12841 aaagagatcc tgcgcgatca actccgcgct atcctgcgtg cctcggcttt cggtaaattg
    12901 cgcattatgt tcccgatgat catctctgtt gaagaagtgc gtgcactgcg caaagagatc
    12961 gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc
    13021 ggcgtaatgg tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt
    13081 gatttcttta gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt
    13141 aatgatatga tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag
    13201 caagttattg atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct
    13261 ggcgatgaac gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc
    13321 gccatttcta tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag
    13381 gtgttagcag agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac
    13441 aagttcattg aagaaaaaac aatctgctaa tccacgagat gcggcccaat ttactgctta
    13501 ggagaagatc atgggtttgt tcgataaact gaaatctctg gtttccgacg acaagaagga
    13561 taccggaact attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt
    13621 gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggt attgctatca aaccaacggg
    13681 taacaaaatg gtcgcgcctg tagacggtac cattggtaaa atctttgaaa ccaatcacgc
    13741 gttctctatc gaatctgata gcggcgttga actgttcgtc cacttcggta tcgacaccgt
    13801 tgaactgaaa ggcgaaggct tcaagcgtat tgcagaagaa ggtcagcgcg tgaaagttgg
    13861 cgatactgtc attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac
    13921 tccggttgtt atctccaaca tggacgaaat caaagaactg atcaaactgc ccggtagcgt
    13981 aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat
    14041 ggcgcccatc g
//
LOCUS       DAJTOR010000101        14011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTOR010000101 DAJTOR010000000
VERSION     DAJTOR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.101"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..1272)
                     /locus_tag="OO249_002669"
     CDS             complement(<1..1272)
                     /locus_tag="OO249_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR5636914.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQVAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPAARQGTNNTQIASTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNAPNFATTMTNALAGKQPKDATLTALAGLATAADRF
                     PYFTGNDVASLATLTKVGRDILAKSTVAAVIE"
     gene            complement(1275..1805)
                     /locus_tag="OO249_002670"
     CDS             complement(1275..1805)
                     /locus_tag="OO249_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCR5636915.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPGD"
     gene            complement(1798..2694)
                     /locus_tag="OO249_002671"
     CDS             complement(1798..2694)
                     /locus_tag="OO249_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR5636916.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCASFTISEGGRNE"
     gene            complement(2698..3027)
                     /locus_tag="OO249_002672"
     CDS             complement(2698..3027)
                     /locus_tag="OO249_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCR5636917.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            complement(3045..3611)
                     /locus_tag="OO249_002673"
     CDS             complement(3045..3611)
                     /locus_tag="OO249_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR5636918.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTSRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(3623..4258)
                     /locus_tag="OO249_002674"
     CDS             complement(3623..4258)
                     /locus_tag="OO249_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR5636919.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     PQKAPDGTAWTPRRRHVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNS
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(4251..4718)
                     /locus_tag="OO249_002675"
     CDS             complement(4251..4718)
                     /locus_tag="OO249_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5636920.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLLEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(4856..5263)
                     /locus_tag="OO249_002676"
     CDS             complement(4856..5263)
                     /locus_tag="OO249_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCR5636921.1"
                     /translation="MKLWPTLGVAFLLTAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            complement(5260..5652)
                     /locus_tag="OO249_002677"
     CDS             complement(5260..5652)
                     /locus_tag="OO249_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR5636922.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQMSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(5649..5972)
                     /locus_tag="OO249_002678"
     CDS             complement(5649..5972)
                     /locus_tag="OO249_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5636923.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(5975..6175)
                     /locus_tag="OO249_002679"
     CDS             complement(5975..6175)
                     /locus_tag="OO249_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCR5636924.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(6175..6669)
                     /locus_tag="OO249_002680"
     CDS             complement(6175..6669)
                     /locus_tag="OO249_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCR5636925.1"
                     /translation="MTIVARTEPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(6837..7583)
                     /locus_tag="OO249_002681"
     CDS             complement(6837..7583)
                     /locus_tag="OO249_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5636926.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKKQQK"
     gene            complement(7629..8501)
                     /locus_tag="OO249_002682"
     CDS             complement(7629..8501)
                     /locus_tag="OO249_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCR5636927.1"
                     /translation="MIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLKNR
                     KYQCEQVNYDTFISYPQLDAWSAHPDFQSRISAQIARQVALDRIMIGFNGTSHADESN
                     FSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQDAR
                     SSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIGGL
                     GVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVEDY
                     GKCAMVTGLKFADS"
     gene            complement(8498..8680)
                     /locus_tag="OO249_002683"
     CDS             complement(8498..8680)
                     /locus_tag="OO249_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCR5636928.1"
                     /translation="MNLVMSDITRNKLGCYMAQQVSLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKKIT"
     gene            complement(8704..9540)
                     /locus_tag="OO249_002684"
                     /pseudo
     CDS             complement(8704..9540)
                     /locus_tag="OO249_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
     gene            9695..11446
                     /locus_tag="OO249_002685"
     CDS             9695..11446
                     /locus_tag="OO249_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCR5636929.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPGQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPSVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            11446..12482
                     /locus_tag="OO249_002686"
                     /pseudo
     CDS             11446..12482
                     /locus_tag="OO249_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(12961..13551)
                     /locus_tag="OO249_002687"
     CDS             complement(12961..13551)
                     /locus_tag="OO249_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5636930.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADKHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKHAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNCGWFSPLTTDKNNT"
     gene            complement(13615..13926)
                     /locus_tag="OO249_002688"
     CDS             complement(13615..13926)
                     /locus_tag="OO249_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5636931.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
BASE COUNT         2841 a         3890 c         3870 g         3410 t
ORIGIN      
        1 ttcgataacg gcagcaacgg tcgatttcgc aagaatatcc cgcccgactt ttgtcagggt
       61 tgccaggctg gcgacatcat tccccgtaaa atacggaaac ctgtctgccg cagtagcaag
      121 cccggccagc gccgtcaggg tggcatcttt cggttgctta cccgcaagcg cgttagtcat
      181 ggtggtcgca aaattcgggg cgtttcccag cgccgccgct aactcgttca gcgtattcag
      241 tgcgtcaggt gacgaatcta caagggcggc aatcgcagcc ataacgaaag ccgtgcttgc
      301 gatttgggta ttattcgttc cctgtcgcgc agctggcgtt gttggcgttc cggtcagcgc
      361 aggactgttt aagggcgctt tcttgttcgt ttcatccatt accgccttaa ccgctttcga
      421 tgtcgcggcc agtgtttcag acgtgctgtt ggtggcacta ctgagctgga ctatcccttt
      481 ttgtgccgtc gtggcgtcct gagcggtata ttttgcgtta gcaaggtcat acgcggcctt
      541 tactgctttc ggcgttgcag cctgcgtttc agacgtgctg ttggtggcgc tactgagctg
      601 gacaaggcct tttcgcgtcg tgctggcatc ctgcgcagta tatttaccgt tagcaagatc
      661 atacgcggcc tttactgctt tcggcgttgc agcctgcgtt tcagacacgc tgttagtggc
      721 gctactgagt tgaacaaagc cttttgcggt cagcgaggcg tccgggtgac gtcgtgactg
      781 ttcatgctct ttcagtttgt catccacgta atccactgtg gccatcacca tggtgttgtc
      841 cacggtaagc gccacggtgg cagtgctgga tacggtcaga atggtgcgaa atgtttgtgc
      901 acgaccggac ccttcggcaa cggctggctt gtaactttcg gcagtatttc ccaccgcgat
      961 taaatcgccg tgctcatcaa atacaccaat ttcccggatc cagaatccgc ccgtttcagg
     1021 aggaataacc agctccgcaa taatgcggtt ctgatgtgtt gcgtccagga tgacgcgatt
     1081 aacagtatgt cgccacacct catgcaccag acgggtctgc ttactgtctg gtgtgggcaa
     1141 cgtgccgcca ccgtcgccca cggccatatg agtcaggcgg acaggcttac catctggcgc
     1201 ggctacctga gctaattttt ttgcacccgt atcggtgata atggttttaa attttcgtgt
     1261 tgtggtactc atgcttaatc gcctggataa atggtaataa cttcaccgtc ataagttgcc
     1321 gccgctgcga aaatatcccc cggaatttcc tgaatgatat tcagccctgt catgtggcgg
     1381 ctgaccgggc gggcatcagc aatcaaccgc tccatttcca gatacatttc ctccgtcacg
     1441 ccactgtcca gcgtgccgac ttcaacggta aatgttcccg gttctccgcc gaactcccac
     1501 cactcagaca cgcgaatgag gtatcccagc ggctcaatgg cccggcgcag tgcgctgatg
     1561 gtccctttgt gtcggtgtat cagccatgca tcacgaataa cctgtcgctt tgtctcttcc
     1621 ggccagttgc gatcccagcg gtcaacggaa aacgcccagg cgagataagg cagcagatgc
     1681 accgggcagg tgtccggcga ccacagcgtg ttgaggtcta ccggaatgtc tgtaatgcgt
     1741 gttccgacgg cttcggcaca acgcatgaaa ttgctggctg atggtggtaa caacgaatta
     1801 ctcattgcgt ccaccttcgc tgatggtgaa tgacgcacag cgcgccgcct gtatgtcgct
     1861 gatggccata ttctgtgtgg gttcgattat ctccacgcgt tgcacaccgt gcacatgcag
     1921 tgcggcagca atggcggaca acgccacgtc ctgaccgata agcccctgct cagccagcca
     1981 cttcctgaac gacgattcag ccgcggccag aataggttcg gattccgggc cggggtaaaa
     2041 gtacagtttt gcattcagcc gccatgtcac gattctggca ctctgtacgg tcaggcggtc
     2101 ggccaccggg cgggtatcct ctgcattcag aacggcgcga acggtattaa gcaacgcctc
     2161 cgttgctgtg ccgtcgccct cagtggacag gatggaaacc gtcacgttgg ccggagacgg
     2221 gctgatggcc cgcgcatcgc gtaccagacc gctggcgctg cgtgcaaaat actcgtatgc
     2281 acctgacggg ccagcaacac tcagaccgtc atatgcccgt tgcgcccgta gtctcagcga
     2341 ggtgtcactt tccatcactg catcggtggt atccgttgcc ggagtgataa ccaggcgctt
     2401 tgtgttcata ttgcccgcga ggttgtccag gtctgtcccg gcgctgtggc ttagcatgca
     2461 ggcgcgtgca ccctcattga cccgctggcg taacagcatt tcacgaaacg acatggtttg
     2521 agcgataacg ttaaggggtt ccgattccag ctccagcgcg gcggaaacgg cttcacgctg
     2581 ttcggcggga taggacgcaa tcatcatggc ctttgtgtca gccagaattg cctcaaagtc
     2641 aggctccgcg atgatggcgg gttccggtaa ctgtgaaagg tcaacggcag gcatgattta
     2701 ctccctcagc gtgatggtta attcaacatt ctgcatggtc tgcatgacag tgcccgacag
     2761 cgtcaccccg gcgcggcctc ctgccttcca gacaacgtcg atagcgtcca gggcaatgcg
     2821 gggttcccat cgtgtcagcg caatcacggc agcactcatg cattgcagac gcgtggtgtt
     2881 attcatgggt tcgtcaatca aatcaggcac aaggctgcca tattcccgtc gcataacccg
     2941 gcttgccagc ggggtggtca ggatgtccct gactgactgt ttcaggtgct ccatatcgtt
     3001 caggtttccc gttccgtccg ggttcattcc tgtgtagcgg gttgtcactg cgggcctcct
     3061 gtcgaatcgc tgccaccttt aacgccaccg tgtttatgcg tatgcactgt gatgccgttt
     3121 gaggtgaaat taccgccgct gtgcgtgata ttgccgctca tctttccccc ttttgtgacg
     3181 tcaagcgtcg ccgttctcag aaggtctgtg cattccacga cgggcgtatc cagtgtcacg
     3241 ctgacggatg cctgcagggt ggctgttttc atgccgctgg cgctcagtgc gcctgcgtcc
     3301 gcgtcgtagc ggaacaccgc gccatccggc gcgctgacca cgatttcttt caggcttttg
     3361 ccgggggccg gaatggcatc actccacagg ctgccaatta tcatggcggt ttccgggttg
     3421 ccgccaatgc aggcaattac cacctgttcg cctggtgatg gcggcagcca cacattgaag
     3481 gctcccgcgc gagaggtgtt ccagcgcagc caacctgttt ccagttcgcc gctgcgaacg
     3541 cgcacgcgcc aggacttctc atcaacttca gagatgatcc cggtgcggat gatattgctc
     3601 agtagccgca tgagttctgc gctcaccgta cagcctccgc aatccggccc agcaccgtgt
     3661 tataaatcag gcgctcatct gcctggctga tacccagcag ctcacgtacc gggtaatcgg
     3721 tgaaaatgcc cggcgcaacc tgatcgcgct caccgaactg atgaacgcgt gcaatacgtg
     3781 cggccacgcc gctgtaaccc accgtcacac cggaagcatc tgcacgggct ttcaggtagc
     3841 gggcggtgcg cagttttacg aacatgggga cgcgctttgt gctgtcctgg ttgatgcgcc
     3901 gggtgcgtat ttccagaaag cggtcgatgt catcccggta aaacgtgcgg atgctgtttt
     3961 tatcctcatc ccacccggtg atggttcgcc cgtatttccc cgtgtcgtga tgccagtttt
     4021 tcagcgtgcg tgcttcgtta ttccagataa agcgaatgcg ctcctgtatc cgggttacgt
     4081 ggcgtctgcg tggtgtccat gcggtcccgt ccggcgcttt ctgtggccgg atacgtgcct
     4141 gctgggcgcg gcgtaaatcc tgtgccagct ttctggcgat gttattgatg gcctgctgat
     4201 tcaggctgtc gcggatggcc tcaaaggttt catccacgcg agtgaatgcc ttatccatcg
     4261 ctttcacccc acgtcacatc ctggaataca tgcgaccagt cgccttcgga agatggcagg
     4321 cggggttttg gctccagcag gtgttctgcc tgcggtgtgc cctgactgct gcgcgtgatg
     4381 cgaacgcgtt cccgcagggg gagcgtaaac aggagatcgg cgctgtcatc gtcattgata
     4441 acggcggaaa atttgatgtc ctgattacgc tcagggttga gcaacaactg tggctgattt
     4501 tcggataacc acgccagcag cggcagcgtg aggtcgtcca gctccccggc gtaatccatg
     4561 acaaacatca ccatctgata gcggtaaaca aacgatgggg tttctccggt cgtttcaatg
     4621 ttgccgctct ccacgaaaat ggtgaatttt tccgggttag cctgacacca tcggcatgaa
     4681 cgggtcatgg cttcacgcag ggaatcagtt ttcagcatgg ttgttatcct cgttgttcag
     4741 tcgttgcagc ctgcgctgtt ccagtaattc aatggcccgt ttatccgcgt tacaggtttc
     4801 cagtgcatcc agaaggcggt cgccccatat accgagattt ccccatgtgg gagtgtcagg
     4861 gaagggggga ggcgttaccg gtatggtcag cgtctgcggt ataagccgga ctgacggcgc
     4921 tggcagtggc gcgttctgcg tgcctgcgca gcctgtcagt aaaacgagcg tcaggcaaag
     4981 cgtgggcgca ttcatctttt gcaatatcgt tgcgtagctg ttcacgtctt acctctccgt
     5041 cctgattgcg ttgctgattt tccacgcgga gttgcgccag cacctgctgc atatcctgta
     5101 ccccggcgct gatgatattc agggtgtcgg cggtactttt cagggtgctg gcctgcgctt
     5161 cgtttctggc gttctcccgg cccagcgacc acgacagacg catggatgtt ccccatccgg
     5221 cagtcagaag gaaagcgacg ccaagcgtgg gccagagctt catgccggat aggctccgtg
     5281 tggtaactga aaatgcggtc cgtctttcag ggtcttccag tcgccgcccc attccaccgg
     5341 aatattcagt tcccggctgg cctgtctgaa tgctgctgcg attttttcgt acagcggcca
     5401 ttcccatgac atctggctgc cgatataagc cacaacatcc acggcatgcc ccgtaaggtg
     5461 gcggctgttc atggtctggc tcttacccgt ggccaccagt tgcttctggc ggtaacggct
     5521 gcgcaaccct tcggtgatac caaaatccac ttccgagatt tccagtgccc gtcgggtcac
     5581 tttcaccaga tcaggattta caccctgcaa attcttttcg ctccggctgc tgaatttaaa
     5641 tgtgttgctc attcgtcctt ctccttcacc ctgcgattaa aggccgcaat aaccttgtcg
     5701 cgtgctttct ctgcacccat aaaaccgatt gatgcgccga taaacgtcac ggcatcttca
     5761 ggaaacccga agaagcgcaa cgacccggcc acggccatgg caagaacgcc gcacgccagc
     5821 gatcccgtta cggtctgaac cagtgttcgt ccgtcataaa ggctcatcag cgcggaaatg
     5881 ctgaccgccg cgcctactgc atacaccgtt ggcaggtggt caaagagcca cgcaataacc
     5941 tgctctgtga tccctgtttg aatggtgctc actgctactc cccccacaac tgaatcattt
     6001 ctcgtttctt cttctccggc tccggcatct ccacttcctg cccggcgtcc agaaatacct
     6061 gctgacagag tccggggttg gcatccagca ccttttcggt gacgccctgc gtcgtgccgt
     6121 agtaccggaa acagagcgaa tccacggtgt cgccttccag tgccttcact ttcatcagca
     6181 caactccgca aagattcgcg ggcggcacag aatgtcagag atggcccagc tcacatcacg
     6241 ccacaaatcc gatgtctgta tatccagagc gtctgcccgg cggtcgccct tgtccgtagt
     6301 gtctgcatca cgataacgct ccagaatcag ggcgcgcgtg gcggtataaa cagcattgcg
     6361 ccagtgccag agattgacgc tttctccgtt aattacgggt gccggaacat cggccagcgt
     6421 ctgatggcca gctgcctgct gttcctgctg ccatgcttcc agctcgcggg taacgtgtgc
     6481 cacagccccg gtggcagtat gcagcaggcg ggaggtggtc acgcgccccg gcagtcgtac
     6541 cgccagacgc agctcgcgca gcacaatatc cggccagaat gcacccgctg aaatgcgggt
     6601 atcgccatca tcggtatcgg tgatgtcgtc ctctgcgggt ccgggttcag ttctggcaac
     6661 tatagtcatg gggttcactc ctgaaaaaaa atcgggcggt gggtgcgcgg tgtaaacggt
     6721 cacggagtca aactggaaca ccgcgcacgc cgcccgctga cggggtcagt cgttaaccgc
     6781 gcttcgcctt ctgcgtcgcg gtggtttttc gtgttgcagg cttccgcgtt gtctttttac
     6841 ttttgctgct ttttgctgct ttcgtcctgc gcctgctgtg cgctggcgtc ttctggttcg
     6901 gctgcggaat cggctttttt cagggcgcgg gaaagggttg caatctcgcg tttcacacct
     6961 gcgttcgggt tcaggtgcat tgcttcgcgc agcagcttca gtgacagggc catgctgtcc
     7021 gcatcactca ggccacggcg ggcaaaggcg cacgctttgc ataatttggc gcgcacttcg
     7081 tccggcatgt cctggtcggt gacaatctcc cggagggtgt ccagtggttc gataaaggcg
     7141 gacaaatccg cgtcggcatc cgtcccggcc tgcgtcagta ccggattaca gatttcttcg
     7201 gtcagtaccg tggcagcagt acggccaaag ttatccggca tgatgaggtt gtgacggact
     7261 acatacgcgc cgatacgcag cgcaagcgga agatcgccac agtcaatcgc ccacaccatc
     7321 agcgtggcaa tcacttcatc ctgctgcccg ccgtcagcct ccagcgttcc ctcaatccag
     7381 ccggaaaaat ccggcaacaa ctcttttttg atggcggctt tcgcgcttct ggcctgtacg
     7441 cccttaagcc gggcctgtgc cagacgcaga cgatacagca cctcttcatg cgcggtacgc
     7501 gcggcgtggt ccacgccttc attcgcccgg cctgcgcgct gtgccatcac gttctgccag
     7561 tgttgttgtg caggagtaat catttttgct ctccgttaca ggcgggcatg atgcccgccg
     7621 tgagttgatt agctgtcggc gaacttcagg ccagtaacca tcgcgcactt gccatagtct
     7681 tcaacgacat aagcgtcatt gatggactgg taggtggcga tgcggttgta ttccggctcg
     7741 tctttcatca ggcgacgcat tgaacctttc tgccagtaaa tcgacaggtt gttgaacgag
     7801 gtgatcagca tcgttgcatc cgggaagaac ggcgcaagga acacgcccag cccgccaatg
     7861 gtgcgcgatg acaggatgag ctgcccggcg agtaattccg cattgggatt ctggccgctg
     7921 atgctgttca gcacgggcag acgcagcgag ttaaacaggt tgcgccccat aatcaccacg
     7981 aggtcgtcag cttccttgtg ccattcatct agcagggatg aacgcgcgtc ctgtaccagt
     8041 gcatcagcgt tcgcatactt acccgcgtgc gccacggtgt tgtccatgtt gcgggaggtc
     8101 agcgtcacgt cattcataac gcgttcgctg gcgtcggttc tgatgtgctc cagccagccc
     8161 acgttaacgt cctgaagcag cttgttggtg ctgaagttgg actcatccgc gtgagacgtg
     8221 ccgttgaaac cgatcatgat gcggtcaagc gccacctgtc gggcaatctg tgcgctgatg
     8281 cgggactgaa aatcagggtg tgccgaccag gcatcaagct gcggatacga aataaacgtg
     8341 tcgtagttca cctgttcaca ctgatatttg cggtttttca gatcaaccac gttattcggg
     8401 ttacggcgtt ttgtgccgtc ataactggta ttcgtgcgcg caatcggtcc tgtggtatcc
     8461 aggaggattt tttcgccttt ctggtcggtc acgccgatca cgttattttt ttcgtaaatt
     8521 cggtactttc ctttgaggcg ttttcaaaac gctgctgcac cgcgggttcc acggtaaatc
     8581 gcgacaccag tgcagatacc gggatattat taagcgacac ctgctgcgcc atatagcaac
     8641 ccagcttgtt gcgggtaata tctgacatca ccagattcat aaaaaatttg ctcctttgtc
     8701 taatcagaag tcggccagct ggtcggaggc tgcgcccgtt gcggtgaagc ggttctgcgg
     8761 atcgccgtcc tgcgtgcgca gtttttcctt cagtgctgcc agctctgtgg tcagtgacgt
     8821 aattttctgg cggtcctgct gatggcgggt ttccagcaca ttaaaacggt cgataatgtc
     8881 ggcctgtgac gttgcgacgc cttccaccgc ttcctgaata cgggaaaaac tggcgtcatc
     8941 cgctttgcgg ccacggccaa taatccccat tacgcggtta aaccactggg tgccttcttc
     9001 ctggcgttgt tctgccattt cgatgatttc agactcgatg gcttcggaga taagcggcgc
     9061 ttcaccctgg atactgttaa acgtcatcac cgcctaacgt tgctgtgccg tgaatttcag
     9121 gcgctcagtg cccaggcttg ccggggtgtc ggtcatcgcc agcccgacca gataggcgcg
     9181 cccgttaacg gagaactgcg ggtgcagttc gatactggaa tagattttct tgccgtccgc
     9241 aacaagctgc ttcatgcgct cggtcggttc gatttctgca tacagcgcag tacgtccggc
     9301 cagcggacct tccgtaatgt cttccgtact cagtgcggtg acatcgccca ttgcggaaaa
     9361 ttcgcttgac gggcatggcg agagatagtg ctcaacgttc acgcgggcag cgtaaacatc
     9421 cgggttgaag ttctcggcgg cttcacgcag atgcaccgga ctgatttcac ggccatcaac
     9481 agttgatccg gagacagcca cgcgaaactt tttgcgggat gtcttttttt cattagccat
     9541 agtttttgcc cctctgactg gttcttcagt catgatggca aagcgtaaca ggctgataca
     9601 aagggctttt gttgtaagaa aacggccaga acagagggtt aaggagaaca gtttcgcgcg
     9661 cgggtaatct tcctgtaatt actcaggggg agcaatgatt caggacgctt ttgtgcgcca
     9721 gcgtgcgcgg caactttact ggcagggtta tccgcccgca gaaatatcac gtctgatggg
     9781 aataaacccg aacacgattt atgcgtggaa aaaacgtgac cagtgggatg aaacgccacc
     9841 cgggcagcgt gtcacgcagt ccatcgatgc gcgcctcatc cagcttactg aaaaacagaa
     9901 taaaacaggc ggtgacttta aggaaataga cctgctgacc cggcagctta aaaagctgca
     9961 tgatggccag ccggatgtga tggccgcagg aaagaaaggc cgggcgaaaa aactcaaaaa
    10021 tcatttcacg ccggaacaga ttgccgcact gcgggaaaaa atcatcagca ggctggagtg
    10081 gcatcagcgg ggctggtttg actccctgac cctttgcagg gaagccggga tacgtaacag
    10141 gatgatcctg aaatcccgac agattggggc gacctggtat tttgcacagg aagcactgct
    10201 gatggcgctg cgtgacgatg tggcgcaacc ttaccagcgt aaccagattt ttttgtctgc
    10261 gtcgcgtcgt caggcgttcc agtttaaaag cattattcag aaggccgcgg ctgaagttga
    10321 tgtggagctg aaagggggcg ataaaatcat cctctccaac ggcgcagagc tgcattttct
    10381 cggcacttct gctgcgtcgg cacagtccta tacgggcaat ttttattttg atgaattttt
    10441 ctgggtcagt cgctttgctg aactgcgcaa ggtggctggc gctatggcaa ccctcagcgg
    10501 actgcggcgc acctacttct ccacgccatc caccgaaacg cacgaggcat acgcctactg
    10561 gaacggcgac cgctggaacg agaaaaaggc ctcgcataaa cgccagcgtt tttctgtgga
    10621 ctggaaaacg ctgcataacg ggcttatctg ccctgaccgg acgtggcggc aaattgtcac
    10681 gctggaagat gtggttaatc acggctggaa acacaccgat atcgacgaaa ttcgtgatga
    10741 aaacaccgaa gacgagttcc tcaatctcta tatgtgtgag tttgtccgcg aaggggaatc
    10801 ggcatttaac ctgaatatcc tgattggctg cggcgttgac ggatacgacg actggaaaga
    10861 ctggaaacct tttgctcccc gcccgatggg gaatcgtccg gtatggattg ggtatgacgc
    10921 aaacggcagc agtggcaacg gcgacagcgg cgctgtgtcc gtggtggttc ctccgtctgt
    10981 tcctggtggc cgttttcgaa cggtggagac gcgacgcgtt caggggctgg agtttgaaga
    11041 acaggccaga gtcattgaag agttcacgtg tcgctacaac gtggaacaca tcggcattga
    11101 tgtgacgggc gggaacgggg aggctgttta tcagatagtg aaacggtttt tccctgccgc
    11161 tattccgtac accttcacgc tgtcatcaaa acggtcgctg gtactgaaaa tgctgcaaat
    11221 aatgcgtgcc gggcggtggg aatacgatcg cgccgaacgc gagctggtcg cggcctttaa
    11281 cgccgtgcgt aaggtgaaaa caccgggcgg ctttatcact tacgaaacgg accgagcgag
    11341 ggggatcagc cacggcgacc ttgcgtgggc aaccatgctt gctgtcatta acgaaccgat
    11401 tggcggcgaa ggagaaaacg agcgtttcac ggttatggag ttctgatgag cagaaaaaat
    11461 aaaaaagtgc gcatgagttc acgcattgat ctcgctgatg cgctcaggaa agaatcatcg
    11521 ctcagtgcat tcacatttga tggtccttat cgcctgacct gctggacaat atgtactgtg
    11581 ctgataacgg gcggtggtat gaaaccccgg tggactggta cggtctggca agagctgccc
    11641 ggcaaacgtc ctggcatcag tctgcgcttt attttaagcg caatgtattg ctcggttgct
    11701 acatcccgca cccgctgctt tcccggcagg atttctcggc gctggcgctg gactggtttg
    11761 tgttcggtaa cgcattcctt gagcttcgaa gcaatatgct cggcgaaccg cttaaattac
    11821 ggcacgccct ggcgaaatac atgcgacgcg gaagcgatct tgaatcatgg aggtatgtgc
    11881 aggatggcaa ggatgcgttt cagtttcgcc caggcaaagt gtgtcacctg atgaatcctg
    11941 acattaacca ggaaatctac ggcatgccgg aatatctcgg cgcattactc tcggccagcc
    12001 tgtctcattc ggcggacatg ttcagaaaac tgtattacga caacggatcc cacgccgggt
    12061 gcatcatcta catcggtgcc gcgcaggtaa accgcgaaag catggactcc ctgaaagaaa
    12121 cgctacaggg tgcgcgtggt ggtggtgcgt ttaaaaacgt gctcattcat gcgcccaacg
    12181 ggggcaaaga gggggtgcaa attttgccgt tccagcagat caccgcaaag gatgagttca
    12241 tgaatgttaa ggcggcatcc cgtgatgatg tgctggctgc gcaccgcgtt ccgccgcaac
    12301 tgatgggggc gatgccgggt gaaaaaagtg cgtttggtga tgtggagaag gccgcgcggg
    12361 tttacgcaat taacgagctg atgcccgtca tggaggccat gaagcacatc aatgactggc
    12421 ttggcgaaga ggtgatccgc tttaaccctt acgcactgtt agacatccag cccacatcct
    12481 gacgcgcttc gcttgtctgc tgcttcgccg gggcataaaa aatttatgcc ccgactctcc
    12541 agctcctgta tcagtcagat aatttcacga cgccttccag tttatcgcca ccatcgacgg
    12601 tcagactctt acgcaatccc accgcgttga ctgcatgttc tcgccgcctc agtgcgattt
    12661 tgacggcctt acctttcacc ccatcaaatc agaatccctc acgtattttt cacgctcagc
    12721 gtgagaaata cggccattct gtcgtgtcgc tgcgacatcg ttaagggaac gctatttacc
    12781 ccctgaaacg cgggctgttc ccccgtcacc tgcgcgcaga aaaagcgcgt ttttttgtgc
    12841 acgcacggat ccttgacgga tccagccgcc acgcgggctg gaagggcaaa aattcgttca
    12901 aaaaaattgt gcaaatgtgc agcagggcga tgtgcgatta tcgccctggt ttgaggggga
    12961 tcaggtgtta tttttatctg tcgtcagtgg gctaaaccat ccacaattta catcgcatga
    13021 ctgatgcgtt ctgtcctcgt gtttctcctg ccacaatgct tttccgccag cctttatgat
    13081 ggctctttca acatcccgct cgtatcgcaa gggctgaaat acgtcattaa tgaaatattc
    13141 gcttatgtcc ggaagcgata ctgcaaaatt atttgtttgt tgcgccgtcc tgctatgcag
    13201 caaagcggca tgctttaatg cgtcctgtgc tttctggaat ttaagtgcat agtcagtggc
    13261 gattttttcc agttcagcga ttcggttgtc tttggtttcc agccaatcaa gtagcgccaa
    13321 tacttcagga tccccgacat ccaacactgt tacgcgtgat ttttcataat gtttgtcagc
    13381 aagagttcgg ccaattttga aatctccatc atcaccataa ccggagcagg cataaacaac
    13441 ctgtgctcca gatattcgct gaattgacat ttcctcgccg caaatagggc atttcggtac
    13501 tggcacaggt gaataacgtt cacgtaatgc ctgatagttg atttcgctca ttaagtcgtt
    13561 ctccttgttt atgttctgag gtggagagca gggcgaactc gccctgatgt tgtgttattc
    13621 aggaaataac gcccggatat ttccggccat ctgactggtt atctgtgcgg ttgatactgg
    13681 ctgtgatgcg gggcgttctg tcctggtttg tgtcactgat aacgcttcat caccagccca
    13741 tgcagccagt cgataagcct ctgccggatt cattttcaga agtgccagcc cggccagaaa
    13801 agccacgcgt tggccgcttt tgcgggcttc tggtgtaagg ctgtccagcc aggcgcatgc
    13861 ttctccttcg ttcttgacgg cggtgggctt cagatagaaa cttatccgtc tggttggagt
    13921 cgtcattggt ttactccttg tccattgcgt acagcccatt aaccagagca aactgtggca
    13981 ccccgtccgc gatgaaagtc gcattaactc c
//
LOCUS       DAJTOR010000102        13847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTOR010000102 DAJTOR010000000
VERSION     DAJTOR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.102"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..569)
                     /locus_tag="OO249_002689"
     CDS             complement(<1..569)
                     /locus_tag="OO249_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5636932.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAA"
     gene            complement(957..1703)
                     /gene="cutC"
                     /locus_tag="OO249_002690"
     CDS             complement(957..1703)
                     /gene="cutC"
                     /locus_tag="OO249_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5636933.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1717..2283)
                     /locus_tag="OO249_002691"
     CDS             complement(1717..2283)
                     /locus_tag="OO249_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5636934.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            2499..4232
                     /gene="argS"
                     /locus_tag="OO249_002692"
     CDS             2499..4232
                     /gene="argS"
                     /locus_tag="OO249_002692"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5636935.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            4409..4897
                     /locus_tag="OO249_002693"
     CDS             4409..4897
                     /locus_tag="OO249_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5636936.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDIEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(5017..5409)
                     /gene="flhe"
                     /locus_tag="OO249_002694"
     CDS             complement(5017..5409)
                     /gene="flhe"
                     /locus_tag="OO249_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5636937.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(5409..7487)
                     /gene="flhA"
                     /locus_tag="OO249_002695"
     CDS             complement(5409..7487)
                     /gene="flhA"
                     /locus_tag="OO249_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCR5636938.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGCEQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(7480..8583)
                     /gene="flhB"
                     /locus_tag="OO249_002696"
                     /pseudo
     CDS             complement(7480..8583)
                     /gene="flhB"
                     /locus_tag="OO249_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(8666..9310)
                     /gene="cheZ"
                     /locus_tag="OO249_002697"
     CDS             complement(8666..9310)
                     /gene="cheZ"
                     /locus_tag="OO249_002697"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5636939.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLAEARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(9321..9710)
                     /gene="cheY"
                     /locus_tag="OO249_002698"
     CDS             complement(9321..9710)
                     /gene="cheY"
                     /locus_tag="OO249_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5636940.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9725..10663)
                     /locus_tag="OO249_002699"
     CDS             complement(9725..10663)
                     /locus_tag="OO249_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HCR5636941.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFG
                     MPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI"
     gene            complement(10666..11478)
                     /gene="cheR"
                     /locus_tag="OO249_002700"
     CDS             complement(10666..11478)
                     /gene="cheR"
                     /locus_tag="OO249_002700"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5636942.1"
                     /translation="MRLPGCSRYSFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
                     LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYR
                     VWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNL
                     TPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMI
                     YFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            complement(11616..12182)
                     /gene="flhC"
                     /locus_tag="OO249_002701"
     CDS             complement(11616..12182)
                     /gene="flhC"
                     /locus_tag="OO249_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5636943.1"
                     /translation="MSEKSIVQEARDIELITLGARLQMLESETQLSRGRLIKLYKELR
                     GSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLYLEQC
                     PQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPS
                     RAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(12185..12535)
                     /gene="flhD"
                     /locus_tag="OO249_002702"
     CDS             complement(12185..12535)
                     /gene="flhD"
                     /locus_tag="OO249_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5636944.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVNDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13313..13741
                     /gene="uspC"
                     /locus_tag="OO249_002703"
     CDS             13313..13741
                     /gene="uspC"
                     /locus_tag="OO249_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR5636945.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         3555 a         3663 c         3234 g         3395 t
ORIGIN      
        1 gcggcctgac tttccagctc atgcgccgcg ttgtcatcca tttttatttc tggcggaaaa
       61 tgggcaatgc ctttatgaca atcgatacag gtttcgccgc ccttttgtgc tttgttatgc
      121 attttctgcg cagattcact ttgcgaggca atatccatgg catcaaaaga atggcaacta
      181 cggcacgttg cagagtcagt tgcttttaat tctttccata ctgtttcggc catttcctgg
      241 cgatgagttt cgaacttatc gtcactgtct attttgccgc taacaaattc atgataaata
      301 tctttagatg cttttaattt agcaaataaa taatccatcc ctgactttgg aatatggcaa
      361 tctgcacatt ccgcacgtat ccctttctgg ttcgaaaagt ggacagttcc ctgatattcc
      421 tcaaaaggtt tactcatcga gtggcaggaa aggcaaaatg ctgtatccga cgttttatgt
      481 aaggcttttt gcgccagcaa taacccgccg ccaccaacca caaccgctat cagcaaaaac
      541 aataacccaa tgcgtttttt ccctcgcata gattcctcta aataaattcc agaacaccga
      601 aatcattgga taattaaata cacaacgaat taaccgcttg cataactcgg cacaacactg
      661 cctgaaataa tcgataaaga atatgattta ttacaatgta atcattaatt gctaaggaat
      721 aaccctgttg ttatttagaa tatatatttg tcagttttag caatttaaac cccataatta
      781 atgtgaatat ataaaatatt tttagcacaa cgctatgttc actgtcaccc gctcaacaga
      841 tcacggttat cgtacgtttt ttatactgct cagggataaa aaaaggcccc tgtcgaaatt
      901 gcaggggcct ggtacgagca agcatcatat tgggcgacat gatgcaacgg taaaaatcat
      961 ttggcctgat ggcgttcaat gattcctttc atttcagcaa ccgccgcccc gtctacgaca
     1021 taacgcgaat actcgtccgc gtgtgcatct gatgacatgg acaatccttg attacgataa
     1081 cgcatcggtg aagcttgcca cgctcccgcg gagctatgga cttccagcac tccggcatcg
     1141 aggaagtggt gcaggttttc tgcacggact cctgctccgg ccataatgat tggagcatca
     1201 cgatgggcaa taagttccat aatttttgat aaaccttgca gtgcatctga tttttgccct
     1261 gatgtcagca cccgggcgat gcccaattcc gcaagattac tgagtgtatt taaagggtta
     1321 gcgcacatat cgaaggcgcg atgaaaagtc actgccagcg gaccggcagc agccattatt
     1381 ttttccattc gtggcatatc gacattcccg tcaacatcga gcacgcccgt caccagtcca
     1441 ggaaaaccta attcgcggac tgtgcgcaca tcctcaagaa tggcggcaaa ctcaccgtcg
     1501 ctgtagcaaa aatcaccacc gcgtgggcgg ataatcggat gcacagggat cgtcacccac
     1561 tggcgcacgg atttcagtac acccaacgac ggcgttaagc ccccctcttt tggggctgcg
     1621 cataattcaa ctctgtctgc gccgttttgc tgcgccgtta gtgcacattc catgctgtaa
     1681 cagcaaattt ccagtaatgc catttttact ccttaattac gccgactgct cgctggcaac
     1741 gatctcttca atggaccacg gatgaaactt aatagttgtt ttgccatcag tgaccgccag
     1801 cgtcggattc ggtaatcgct catactcacc ttttggcgaa ctggttttca ctgagatccc
     1861 cggtaggctg agtccttcat cagaaagcaa tgccagcgca cgggcattca gcgtttctgg
     1921 atcacccggc agaacgattt caatatgttc ccaaccttcg tgtgggtaac gtttttcccc
     1981 gggccacggt agctccacaa tggaaaactg ccagtgcgca acctgtaccg gttcatgcaa
     2041 tttaaacaga caaatcggtc tgccattgat catattttct gacaaaagct cgccacactg
     2101 ttcaaaaccg cgacgccagc gctcagcggt gacgttttga tggcaacgca aagaaatgtg
     2161 atcggcagtc agcggagtga tatccaaccc cagacggcgg gagagttcat ctaatgcgtg
     2221 ggtaaatcgc ggtaaatccg atgcaatatc ctgcagctcg tcgatagatt gccagttcgc
     2281 cataatcact cttcgtcttt cagtaaaagc gttaatttac cctgttgccc tgcgccaacc
     2341 aaccgctgat ttcacgccgc ttctgatgca atagtgaaaa cggcaatacg ccacgcgcac
     2401 gttgctgacg aaaacagcca tttgcagtat actcccgccc taatttcttt aactggtgcg
     2461 ggcaattttt gctcgcttca tcaatgtaag gtattccggt gaatattcag gctcttctct
     2521 cagaaaaagt ccgtcaggcc atgattgcgg caggcgcgcc tgcggattgc gaaccgcagg
     2581 ttcgtcagtc agcaaaagtt cagttcggcg actatcaggc taacggcatg atggcagttg
     2641 ctaaaaaact gggtatggca ccgcgacaat tagcagagca ggtgctgact catctggatc
     2701 ttaacggtat cgccagcaaa gttgagatcg ccggtccagg ctttatcaac attttccttg
     2761 atccggcatt cctggctgaa catgttcagc aggcgctggc gtccgatcgt ctcggtgttt
     2821 ctacgccaga aaaacagacc attgtggttg actactctgc gccaaacgtg gcgaaagaga
     2881 tgcatgtcgg tcacctgcgc tctaccatta ttggtgacgc agcagtgcgt actctggagt
     2941 tcctcggtca caaagtgatt cgcgcaaacc acgtcggcga ctggggcact cagttcggta
     3001 tgctgattgc atggctggaa aagcagcagc aggaaaacgc cggtgaaatg gagctggctg
     3061 accttgaagg tttctaccgc gatgcgaaaa agcattacga cgaagatgaa gagtttgccg
     3121 agcgtgcacg taactacgtg gtaaaactgc aaagcggtga cgaatatttc cgcgagatgt
     3181 ggcgcaaact ggtcgacatc accatgacgc agaaccagat cacctacgat cgtctcaacg
     3241 tgacgctgac ccgtgatgac gtgatgggcg aaagcctcta caacccaatg ctgccaggaa
     3301 ttgtggcgga tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg accgtcgtat
     3361 tccttgatga gtttaaaaac aaggaaggcg aaccgatggg cgtgatcatt cagaagaaag
     3421 atggcggcta tctctacacc accactgata tcgcctgtgc gaaatatcgt tatgaaacac
     3481 tgcatgccga tcgcgtgctg tattacatcg actcccgtca gcatcaacac ctgatgcagg
     3541 catgggcgat cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg gaacaccaca
     3601 tgttcggcat gatgctgggt aaagacggca aaccgttcaa aacccgcgcg ggtggtacag
     3661 tgaaactggc cgatctgctg gatgaagcct tggaacgtgc acgccgtctg gtggcagaaa
     3721 agaacccgga tatgccagct gacgagctgg aaaaactggc taacgcggtt ggtattggtg
     3781 cggtgaaata tgcggatctc tccaaaaacc gcaccacgga ctacatcttc gactgggaca
     3841 acatgctggc gtttgagggt aataccgcgc catacatgca gtatgcttac acgcgtgtat
     3901 tgtccgtgtt ccgtaaggca gaaattaacg aagagcaact ggctgcagct ccggtaatca
     3961 tccgtgaaga tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa gaaaccctca
     4021 ccgtggttgc ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac gatctggctg
     4081 gtctgttctc tggcttctac gagcactgcc cgatcctcag cgcagaaaac gaagaagtgc
     4141 gtaacagccg tctgaaactg gcacaactga cagcgaagac gctaaagctg ggtctggata
     4201 cgctgggtat tgagacagta gagcgtatgt aatcgatttt tcgtgagagt aaagcctgat
     4261 cagggttacc tgatcaggct tttttattgc catctaaatg tattctgaaa atggacatgc
     4321 cattgttttc tgactgttgg ataagaggcc agaagcgtaa tatccggccc cagggaaacg
     4381 ataacggttg aatttaagga ataccgcagt gtttaaattt cttgtattaa cattaggcat
     4441 tatctcttgc caggcttacg cagaagatac ggttatagta aacgaccatg acatttcagc
     4501 catcaaagat tgttggcaaa aaaattcaga tgatgatact gacattaacg tgatcaaatc
     4561 atgcctgcga caagaataca atctcgtcga tgcgcaatta aataaagcct atggtgaagc
     4621 ttatcgttat atagaacaag tgccacgcac aggtgtaaaa aaacctgata tcgaacaact
     4681 taacttgctt aaaaaatcac agcgagcctg gctggatttt agggacaaag aatgtgaatt
     4741 aatcctttca aatgaggacg ttcaggattt aagtgaccct tattctgaat cagaatggct
     4801 ctcatgtatg atcatacaga ccaatacgcg aactcgccag ttgcagctat accgtaactc
     4861 tgaagatttt tatccaagcc ctttgacaag aggataattc acatcttttt ggcatgtttt
     4921 gttgcaagct attcctgata aataattgca acaagacatc gagccttttt cactgagtta
     4981 ttaaacatac tcgcgagcgc gtaatttttt tgtccttcag cgataattca caatcacttc
     5041 attacgttgt acctttagcg gtggaattaa ccgcccaccg cctggcactt cccagataaa
     5101 tcgcaacggt tctgctgccg ctataccgga aaaggccacg gtggttccgc tctgcccctc
     5161 taattcgaca caacgagatt gcgaacacaa gcgaacccgc agtcctgaag gtgtcgggcc
     5221 gataagctga taacgccacg ctaccagcgt catcaatcct gaagcaggtt gtcgcgtaga
     5281 aagaggcgca gacgacatcg actcaccgcg atgatttagc gtaattccta cactacttgc
     5341 ctgccacatc ccctccccgg cggcttgcac cagcagcgga aacaataata ttgctaataa
     5401 ggttctcatt atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt atcagacagt
     5461 tccagattcg acaggaccac taactgcggc aagctgcggc gcaggaagcg agacaataat
     5521 ggtcgcagcg cgtggttcac caacaatact ggcggcgcac ccagcatctc ctgacgggat
     5581 agcgcttcct gagtttgcgc cagtaaacga tccgccagcc ctggctccag tcctcccccg
     5641 ccctgcagcg cctgtagtaa caaacgttcc agcggtgtat cgaggccaat aacatggact
     5701 tcatctttgc caggaaacca ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg
     5761 gcggttaatt catgtggatc gctttggatg ggcgcatgtt ccgccagcgt ttcgagaatg
     5821 gtgcgcatat cgcgaatcgg tactttttca tcgaggagat tttgcagcac tttatgcagt
     5881 gtggtgagcg tgacgacgcc aggaacgaga tcttccgtca gctttggcat ctcctgggcg
     5941 acgcgatcca acagctgttg cgcctcctga cgaccaaaca gctctgcggc atgctggcta
     6001 atgaggtggt taagatgcgt tgctaccacc gtgctggcct caaccactgt gtacccctga
     6061 atctgcgcct gttcttttag cgcactttca atccagatag cattcaggcc aaatgccgga
     6121 tcgacggtcg cctcaccagg taacgtcccg gcagcggttc cagggttaat cgccaaccag
     6181 cgccccggat aagcatcacc actgccaatc tccacgcctt tcatcaaaat gcgatagcgg
     6241 gcaggttgca gatccatatt gtcgcgaatg tgcaccactg gcggcagaaa tcccatctcc
     6301 tgggcaaatt tcttgcggat actgcgtata cggcccaaca actcaccatc ctgctggaaa
     6361 tcgaccatcg ggatcagtcg ataacccact tccattccca gagaatcttc cagttgtaca
     6421 tcgttccacg tcgcttcgac aacggtatta ttctctgcca tttttaccgg tttgggttcg
     6481 gcaggcgctt tttgttcgca tccgcgtatc caccaggcca gccccagcaa tccggcagtg
     6541 aacagcaaaa ataccaggtt cggcattcca ggcaccaagc cgagtaaacc gagcacggcg
     6601 gcgcttaaca acataacgct tgggttactg aaaagctgat tcaccatctg ctcgccaaca
     6661 tcctgatcgg tgctgacacg cgtaacgatg acccccgcgg cggtagaaat caccagcgcc
     6721 ggaatttgtg ccaccagacc gtcgccaatg gtcaatagcg tataactttc cgccgcgtgt
     6781 cccatgctca tgccatgttg cagcacgccg accagcaacc cgccgacaac gttaatgacc
     6841 atgatgagga tcccggcgat ggcatcgccg cgaacaaact tacttgcccc gtccattgag
     6901 ccgtaaaaat cggcttcctg agtcacttcg gagcggcgtt ttttcgcctc atcttcacca
     6961 atcaatccag cgttaaggtc ggcgtcaatc gccatctgct tacccggcat accatcgaga
     7021 acaaagcgcg cacccacttc tgcgatacgc ccggcacctt tggtaatgac cataaagttg
     7081 atgatcacga gaatgacaaa caccacgata cctatagcga aattgccacc aacgaggaag
     7141 tgaccgaacg cttcgaccac cttccctgcc gccgccgcgc cggtatgccc ttccatcaaa
     7201 atgatacggg ttgaagccac gttaagtgcc agacgcaaca acgtagtaaa caacagaatg
     7261 gtcggaaacg cagcaaactc aagcgtgcgc tgggtaaaca tcgccaccag caacaccatg
     7321 atcgacaagg caatattgaa ggtaaacaac aggtcgagta tgaatgcggg cagtggcagc
     7381 accatcatcg acaagatcaa caggatcaaa atcggtccgg caaggatctg ccattgtgtc
     7441 gatttcaggt ttgcgggcag gcgcagcatc gcggccagat tactcatggg tcggtttctc
     7501 gttaataaaa tccagggctt ccggcaccgg aagatgagta ggttgtacag ggcgctgtcc
     7561 accagccaga cgccagcgtt tcagttgcca gatccaggcc agcacttccg ccaccgcggc
     7621 gtacagttga cccgggattt gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag
     7681 cggcggcgct tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc
     7741 gaccagccct gcacctttag cgaccacttt cggtgcgctc attttgtttt cgtcatactg
     7801 caacgctacc gaatagtggg tcgggttatt gacaatgaca tccgctttcg gcacatcggc
     7861 catcatccga cgccgtgcgg cagctcgctg catctgacgg atccgtcctt taacatgggg
     7921 gtcgccttcg ctttgtttga actcatcacg aatatcctgc cgcgacattc gcagcttttt
     7981 caggtggctg aagatttgga aaaagacgtc aaatcccacc attggaatga caccaagcac
     8041 caccagcagt gcgcatagcc ctaccaaatc catcgcatta cccatggcgg taatcggaga
     8101 ctcggccagt aagcgcatca tctgcggcca gtgatgccag agaaaaaacc ccgtcacgct
     8161 gccaaccaag atggttttca aaattgcttt aagcaactcc gcgccagtct gtgccgagaa
     8221 catccgttta atgcccggta gcgggttgag tttggaaaac ttcggctgca aggattttcc
     8281 gctaaatacc agccccccca gcatgaccgg agaaatgagc gctaccagca ccacgccgct
     8341 aatcagcggt agcagcgcca gcatggcttc tctgatcagc agaataatct gcccgaggat
     8401 cagattcggg tctttgataa tactgtgatc aaaatgcagc ccagcggaga gcatgcccga
     8461 caatcgacgg gccagcgaca caccgccgaa ccagataaca cacacgccca ccagcaaaat
     8521 cagcagtgag gtcagttcac gggaacgcgg gatttagggc ttttgctgcc acacatcaag
     8581 catagtgtgc gtttgtcgga tgcggcgtca tcgccttatc agaccgcctg atatgacgtg
     8641 gtcacgccac atcaggcaat acaaatcaaa atccaagact atccaacaaa tcgtccacct
     8701 gatcctgact ggctaccaca ccggctttgc tggtatcgac ctgaggtcca ttaagcaaac
     8761 tctggttttc acgttttgga cgcgactcct gttccgggat gttttccaac agcaccatca
     8821 gcaactggcg ttcgatctcc tgaatgacat ccatcatccg cttaataacc tgcccggtga
     8881 gatcctgaaa atcctgcgcc atcatgattt ccagcagttg cgcgttagta aagctggtat
     8941 gcgcgggtac atctgccaga aattgtcgtg tatctgttac cagttcacgg gcctcggcaa
     9001 ggtcaatcgg atcggcaaac cagtcatccc aacgttgggt taacgctttt gctgatttct
     9061 ccatttgatc ctgatgcggt tgtgacgcct caacactgtt cagcgcccgc tccgcagcct
     9121 gggcggtcat ctgcacaaca tagtacaaac gatcgcgcgc atcggggatg gcttccgccg
     9181 cttcggcaat ggcctgatcc agccccagtt cccgcaaact gtcgcgcagc atacgcgtca
     9241 ggctgccgat gcgcgcaatg atatcgccag ctgaatgctc gtcagcaggt ttgattgatg
     9301 gttgcatcat agtcgcatcc tcacatgtcc agtttatcaa agattttgtt gagtttttcc
     9361 tccagcgtcg cggcggtaaa tggcttcacc acatagccac tggcccccgc ttgcgccgca
     9421 gcaatgatgt tctctttctt cgcttctgca gtcaccatta acactggcaa tgccgacatc
     9481 gcgccatccg cacgaattgt tttcagcaat tccaggccat ccatattggg catgttccag
     9541 tcggagataa caaatccata accgcctgcc tgcaacttat tgagagcgtc gacgccatct
     9601 tccgcttcct caacattatt gaatcccagc tctttcagca ggttacgcac tatgcgtcgc
     9661 atggtggaaa agtcatccac aaccaaaaat ttaagttctt tatccgccat ttcacactcc
     9721 tgatttaaat acgtatcgcc tgtccggcac taatttttgc caacatttgc tggcttacct
     9781 ggctaagatc gaccacttcg cagacaccac ccatattgat ggcctcgcgc ggcatgccga
     9841 acaccacgca acttgcttcg ttttgcgcaa gggtccatgc ccccgcctga cgcatcgcca
     9901 acattcccac cgggccgtcg tgaattttga tttgataatt tgcgccacta cgcgccagtt
     9961 ccatatgccg atcgcccggc gcaatatagg catgccccgg caagacacgt tctccgtctt
    10021 cggcttcttt aaccccgatc tggcaaagct tattaagtct gtcggcaaaa gagcgggtga
    10081 aaccgggcgg catatgttgg gtaattaaca atgccgggct ggaaagcggc aacggttgca
    10141 gtacgtgacg aattgcctca gttccacccg ttgaagcacc aatcgcaatc agtttttcag
    10201 aactcaacaa cggacccgcc ttcagcgttg tcggtgctga caatggctta tgtgctgcaa
    10261 ggctcgcctt tgctgccgta cgcacctttt cagcaatcat ttcgctatac gccagcattc
    10321 cttcgcgaat acccagttgc ggtttggtga caaaatctat tgcccccagc tccagcgcgc
    10381 gcagcgtgac ttctgatcct ttgccggtca aggaagaaac catcacaacg ggcattggac
    10441 gcaaacgcat taatttttcg aggaaatcca gtccgtccat ccgcggcatt tcaacatcca
    10501 gcgtcagcac atcgggattg aatttcttaa tcaagtcacg cgcgaccagc ggatcaggcg
    10561 cggtcgccac catttccatg tcgctatggc tgttgatgat ttctgtcatg atctggcgca
    10621 tcagtgccga atcatcgaca gataacaccc tgattttgct catcgttaat ccttacttag
    10681 cgcatacacc gtctgaccac gcagcgtgaa gcggcgctca aggtggctaa agttttcaga
    10741 gtgacccgca aacagcaatc cgtcgggttt aaggagcgga acaaagcggc gcaaaatctc
    10801 ctgctgggta ttttgatcga agtagatcat gacgttacga cagaagatcg catcaaacgg
    10861 ccccggcacg gtgtactgtt tcgccagtag attcagcggg gcaaaatcaa catagttcgc
    10921 cagctcctga cgcacgcgta ccagcccttc atgcggtccc gtccctcgca tgaaataccg
    10981 ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg cgataaatac cgcttctggc
    11041 tttttccagc acttcggtgt cgatatcact ggcaaacact ttccagcgtc cgggcgcggt
    11101 gcccaatgtg tcagccagcg tcatcgcaat gctgtacggc tcttcgccgg ttgaagccgc
    11161 cgcgctccat acgcgatact cgccagaacg gcgacgtgcg tgatccgcga gcagagggaa
    11221 atgatgtgcc tcacggaaaa atgccgtcag attcgtggtc agcgaattga taaacgcctg
    11281 ccactcaccg ctgtgctgat tagattccag caagttcaga taatgaccga aatccgtcag
    11341 tcccagcgaa cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt tatgatcggc
    11401 caggacgatc ccggctcgtt gatagatcaa ttgacttatc cgccgaaaac tgtaccgaga
    11461 acaaccaggt aacctaataa gataagcacg acatcatcct tccactgttg accatgacag
    11521 gatgttcagt cgtcaggcgt taacgcgcga ttggggcaaa aaaaagcagc ggtacgtcgt
    11581 taccgctgct ggcatgttgc gccacaccgt atcagttaaa cagcctgtac tctctgttca
    11641 tccagcagtt gtgggataat atcggcagga ttctgggaaa gtttacgtct ttttactgcc
    11701 cgggatggcg gttgacataa gctgcaggca aagctgccaa caggctggtg agcgtgggta
    11761 ataaaattgc cgccgcagca gttgcagctg gaaagttgca gtaatccact ttcaacaaac
    11821 cgcaccaatg tccaggcacg ggttaatgcc agcagtggtc cttcttctgc ttgtgggcac
    11881 tgttcaaggt ataaacggta ggctttgatc accgcatcga cgccattaca caaacctgtt
    11941 ttcagtaaaa actgccatgc attacagaac atcgaagcat gaacgttttg ttcccaggtc
    12001 ataaaccagt cggttgagaa tggcagcatg cctttcggcg gtgggcttcc gcgcagttct
    12061 ttataaagtt ttatcaggcg tccgcgactt aactgtgttt cgctttccag catctgcaaa
    12121 cgagcgccca gggtgatcaa ttcaatatcc cgcgcttcct gaacaatgct tttttcactc
    12181 atgatcaggc ccttttcttg cgcagcgctt cttcaggctg attaacatca ttcagcaagc
    12241 gtgttgagag catgatgccg gtatgaattt gctggagatc gttaacgcgg gaatcttgcg
    12301 tcaactgagt aatcgtctgg tggctgtcaa aacggaagtg acaaaccagt tgattggttt
    12361 ctgccagctt aaccatttgc ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat
    12421 ttatgccgag acgaaacata gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta
    12481 gtaaatatga caagttgatg tcataaatgt gtttcagcaa ctcggaggta tgcattattc
    12541 ccacccagaa taaccaactt tatttttatg cggtttcacc gcaccccgtg atgtcgccgg
    12601 gaagccccgg taaaaaataa ttagcattag aatagtgcga taagctgcaa taagcagaac
    12661 cacctttttg gtttaatatg tccttacaaa tagaaatggg tctttacact tatctaagat
    12721 ttttcctaaa tcgacgcaac tgtactcgtc actacacgca catacaacgg agggggctgc
    12781 gattttcaat aatgtgtgat gcagatcaca caaaacactc aattacttaa cataaatgta
    12841 taagccatac ttttgttttg ggtgtatttc aatctgttaa aaagtttttc gctacgctag
    12901 caagcaaaaa tgaaactgga ataatcgaaa tgggatgttg cgcacagtta aaataactca
    12961 ccgtaaataa tcatctgcta taaataatca ctttcatgca ataccagata agctattttt
    13021 aaacagacac ttaccgcaca acaaactaat taaatacaaa gaaaggcaac aatcaactta
    13081 atgttaatga aatgttttac atatattaac cacttgttta atgcggagaa tcagtacact
    13141 attttcttat aattacattt gaaattatat gatcaccggg agtgattgat taattgtctt
    13201 tactaataat cagactaata tttacctgtt tgaccgagtt gggattgcgt cgtttctcca
    13261 ttaggagtaa agctttaatg tcacctgaag ttcacagaat aaggaacagg ttatgagcta
    13321 tagcaatatt cttgtcgctg ttgcggttac accggaaagt cagcaactgc tggcaaaagc
    13381 ggtatctatc gccaggccag taaagggaca catcagttta attactctcg cttccgaccc
    13441 ggaaatgtac aatcaattag ccgcgccgat gcttgaagat ttacgcagcg tgatgcaaga
    13501 agaaacgcaa agctttcttg ataagttaat tcaggatgcg gggtatcccg ttgacaagac
    13561 gtttattgcc tacggtgagt taagcgaaca tattctggaa gtatgtcaca aacatcattt
    13621 cgatttggtg atttgcggta atcacaatca cagtttcttt tcgcgagcat cctgctcggc
    13681 gaaaagagtg attgcctcaa gtgaggtcga tgtgctgtta gttccactta cgggtgatta
    13741 accgctccta cgcaagcttt ggaaaggtag caactttatc gcgctgctgg ctttccgcgc
    13801 ttcgcggaac tatctgcttt aggtcgctaa tgaagcactc ctgccag
//
LOCUS       DAJTOR010000103        13589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTOR010000103 DAJTOR010000000
VERSION     DAJTOR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.103"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            308..643
                     /locus_tag="OO249_002704"
     CDS             308..643
                     /locus_tag="OO249_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5636946.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDTEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            803..1246
                     /gene="yjdN"
                     /locus_tag="OO249_002705"
     CDS             803..1246
                     /gene="yjdN"
                     /locus_tag="OO249_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5636947.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1379..2167
                     /gene="phnC"
                     /locus_tag="OO249_002706"
     CDS             1379..2167
                     /gene="phnC"
                     /locus_tag="OO249_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5636948.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2192..3208
                     /gene="phnD"
                     /locus_tag="OO249_002707"
                     /pseudo
     CDS             2192..3208
                     /gene="phnD"
                     /locus_tag="OO249_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
     gene            3334..4113
                     /gene="phnE"
                     /locus_tag="OO249_002708"
     CDS             3334..4113
                     /gene="phnE"
                     /locus_tag="OO249_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR5636949.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4134..4859
                     /gene="phnF"
                     /locus_tag="OO249_002709"
     CDS             4134..4859
                     /gene="phnF"
                     /locus_tag="OO249_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR5636950.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4860..5312
                     /gene="phnG"
                     /locus_tag="OO249_002710"
     CDS             4860..5312
                     /gene="phnG"
                     /locus_tag="OO249_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR5636951.1"
                     /translation="MHADIATRQHWMSMLAHSQPAELAARLNALNITANYEVIRTAET
                     GLVQIQPRMGGTGERFFAGDATLTRAAVRLTDGPLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5309..5893
                     /gene="phnH"
                     /locus_tag="OO249_002711"
     CDS             5309..5893
                     /gene="phnH"
                     /locus_tag="OO249_002711"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR5636952.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQSELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5893..6951
                     /gene="phnI"
                     /locus_tag="OO249_002712"
     CDS             5893..6951
                     /gene="phnI"
                     /locus_tag="OO249_002712"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR5636953.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVD
                     FQAELELLKRLQQEQNHG"
     gene            6944..7789
                     /gene="phnJ"
                     /locus_tag="OO249_002713"
     CDS             6944..7789
                     /gene="phnJ"
                     /locus_tag="OO249_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR5636954.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7786..8544
                     /gene="phnK"
                     /locus_tag="OO249_002714"
     CDS             7786..8544
                     /gene="phnK"
                     /locus_tag="OO249_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR5636955.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEGGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8655..9335
                     /gene="phnL"
                     /locus_tag="OO249_002715"
     CDS             8655..9335
                     /gene="phnL"
                     /locus_tag="OO249_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR5636956.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9332..10468
                     /gene="phnM"
                     /locus_tag="OO249_002716"
     CDS             9332..10468
                     /gene="phnM"
                     /locus_tag="OO249_002716"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR5636957.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLNAVAIGDVR
                     DGGDQLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10468..11025
                     /gene="phnN"
                     /locus_tag="OO249_002717"
     CDS             10468..11025
                     /gene="phnN"
                     /locus_tag="OO249_002717"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR5636958.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            11012..11461
                     /gene="phnO"
                     /locus_tag="OO249_002718"
     CDS             11012..11461
                     /gene="phnO"
                     /locus_tag="OO249_002718"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR5636959.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYPSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLNVSSKL
                     LAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11463..12221
                     /gene="phnP"
                     /locus_tag="OO249_002719"
     CDS             11463..12221
                     /gene="phnP"
                     /locus_tag="OO249_002719"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR5636960.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWPPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12368..12697)
                     /gene="yjdP"
                     /locus_tag="OO249_002720"
     CDS             complement(12368..12697)
                     /gene="yjdP"
                     /locus_tag="OO249_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR5636961.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12766..13083)
                     /locus_tag="OO249_002721"
     CDS             complement(12766..13083)
                     /locus_tag="OO249_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR5636962.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13336..>13589
                     /locus_tag="OO249_002722"
     CDS             13336..>13589
                     /locus_tag="OO249_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636963.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPY"
BASE COUNT         3059 a         4028 c         3747 g         2755 t
ORIGIN      
        1 aattttatat ttagtgtgtt gttttttatc agaataaata acgtcttctg atgcgtttaa
       61 aacgtcagaa agataaaaat atcatgtgaa taaaaaaaga acaggtagag cattaacatt
      121 atcttaaata ataaatagag gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga
      181 aaacgatcgt cgtctaaaat cagcagtacc cccgacaaac tcagggattt tgtgtataat
      241 tgcggccttt ttcggcaatc tgccgttttt tggcgctttt gccctgctga cttttgagga
      301 aatccacatg tcattaccac actgcccaaa atgcaactcc gaatacactt acgaagataa
      361 cggcatgtac atctgcccgg aatgtgccta cgaatggaac gacacagaac ctgcacagga
      421 aagcgacgag ctgatcgtta aagatgctaa cggcaatctg ctggctgacg gcgacagcgt
      481 taccatcatt aaagatctga aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa
      541 agtgaaaaac atccgcctgg ttgaaggcga ccataacatc gattgcaaaa tcgacggttt
      601 tggtccgatg aaactgaaat ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta
      661 agccggataa ggcgctcgcg ccacatccgg caacgatgcc gactgcctga tgcgacgctg
      721 accgcgcctt atcaggcttc cctctcatct gtataaattt cgaactacac ttaactggct
      781 tctcttaact gaggtcacca tcatgccgtt aagtccctac ctctcttttg ccggtaactg
      841 ttccgacgcg attgcctatt atcaacgtac gttgggcgcg gaactgctct ataaaatcag
      901 cttcggcgaa atgccaaaat cagcgcagga cagcgccgag aactgccctt ccggaatgca
      961 atttcccgat accgccatcg cccacgccaa cgtgcgcatt gccggaagcg acatcatgat
     1021 gagcgatgct ataccgtcag gaaaagccag ctactccggc tttacgctgg tgctcgattc
     1081 gcaacaggtc gaagaaggaa aacgctggtt tgataacctt gccgctaacg gaaaaattga
     1141 aatggcctgg caggaaactt tctgggcgca tggcttcggc aaagtcaccg ataaatttgg
     1201 cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg caataacccg ccgggaggcc
     1261 cgccctcccg cactgtcatc gaattcccgt taactcttca tctgttagtc acttttaatt
     1321 aaccaaatcg tcacaataat ccgccacgat ggagccactt ttttagggag gctgcatcat
     1381 gcaaacgatt atccgtgtcg agaagctcgc caaaaccttc aatcagcatc aagcgctgca
     1441 tgcggttgat ctgaacattc atcacagtga aatggtggct ctgcttgggc cgtcgggttc
     1501 cggcaaatcc acacttttac gtcacttaag cggtttgatt accggcgata aatccgccgg
     1561 cagccatatc gagctgctgg gccgcacagt ccagcgcgaa ggccgtctgg cgcgcgatat
     1621 ccgcaaaagc cgcgcccata ccggctacat cttccaacaa ttcaacctgg tgaatcgcct
     1681 gagcgtactg gagaacgtgc tgattggcgc gctcggcagc acgccgttct ggcgcacctg
     1741 ttttagctgg ttcacccgcg agcagaaaca acgcgcgtta caggcgctga cccgcgttgg
     1801 catggtgcat tttgcccatc aacgcgtttc caccctctcc ggcggacagc agcagcgtgt
     1861 ggcgattgcc cgcgcgctga tgcagcaggc gaaggtgatt ctggccgatg aacccatcgc
     1921 ctcgctggac ccggaatccg cccgcatcgt gatggacacc ctgcgcgaca tcaatcagaa
     1981 cgacggcatc accgtagtcg tcacgctgca tcaggtggat tacgccctgc gctactgcga
     2041 acgcatcgtc gccctgcgcc aggggcacgt tttctacgac ggcagcagcc aacagtttga
     2101 taacgaacgt tttgaccatc tctaccgcag cattaatcgc atcgaagaga acgcgaaagc
     2161 tgcctgacat ccccatcatt gaggaaaacg aatgaacgct aagataattg cctcgctggc
     2221 cttcaccagc atgttcagcc tcagcaccct gttaagcccg gcacacgccg aagagcagga
     2281 aaaggcgctg aatttcggca ttatttcaac ggaatcacag caaaacctga aaccgcaata
     2341 gacgccattc ttacaggata tggagaagaa gctgggcgtg aaggtgaacg ccttctttgc
     2401 cccagactac gcaggcatta tccagggaat gcgcttcaat aaagtggata tcgcctggta
     2461 cggcaacctg tcggcaatgg aagcggtgga tcgcgccaac ggccaggtct tcgcccagac
     2521 ggtcgcggcg gatggatcgc caggttactg gagcgtgttg atcgtcaaca aagatagtcc
     2581 gatcaacaac ctgaacgatc tgctggcgaa gcggaaagat ctcaccttcg gcaatggcga
     2641 tcctaactcc acctctggct tcctcgtccc cggttactac gtctttgcca aaaacaatat
     2701 ctccgccagc gactttaagc gcaccgtcaa cgctgggcat gaaaccaacg cgctggccgt
     2761 cgccaacaag caggtggatg ttgccaccaa caacaccgaa aacctcgaca agctgaaaac
     2821 ctccgcgcca gagaagctga aagaactgaa ggtgatctgg aagtcgccgc tgatcccagg
     2881 cgatccgatc gtctggcgca agaatctctc cgaaaccacc aaagacaaga tctacgactt
     2941 ctttatgaac tacggaaaaa cgccggaaga gaaagcggtg ctggaacgcc tgggctgggc
     3001 cccgttccgc gcctccagcg acctgcaact ggtgccgatt cgccagctcg cactgtttaa
     3061 agagatgcag ggcgtaaaaa gcaataaagg actgaatgag caggacaagc tggcgaaaac
     3121 caccgagatt caggcgcagc tggatgactt ggaccgcctg aacaacgcgt taagcgcgat
     3181 gagttcggtg agtaaagcgg tgcagtaatg tgctcgatcg gtcccctctc ccttgaggga
     3241 gagggttagg gtgaggggga aatgcgcacg ctgatgccct catcccggcc ctctcccaca
     3301 gggagaggga gaaaacatta aacggagcca aacatgcaaa ccatcaccat cgccccaccc
     3361 aagcgtagct ggttctcgct tctgagctgg gccgttgttc tcgccgtgct ggtcgtctcg
     3421 tggcagggcg cggaaatggc cccgcttacg ctgattaaag acggcggcaa catggcaacc
     3481 ttcgttgccg acttcttccc gcccgatttc agccagtggc aggattacct caccgaaatg
     3541 gccgtcacgc tgcaaatcgc cgtctggggc accgcgctgg cagtggttct ctccatcccc
     3601 ttcggtctga tgagcgccga aaacctggtg ccgtggtggg tttaccagcc cgttcgccgc
     3661 ctgatggatg cctgccgcgc cattaacgaa atggtcttcg ccatgctgtt cgtggtcgcc
     3721 gtcggtctcg gcccgttcgc tggcgtgctg gcgctattta tccacaccac cggcgtgctc
     3781 tccaagctgc tttccgaagc ggtggaagcg atcgaacccg gcccggtaga aggcattcgc
     3841 gccaccggcg ccaacaagct ggaagagatc ctttacggcg tgctgccaca ggtaatgccg
     3901 ctgctgatct cctactccct ctatcgcttc gaatccaacg tccgctcggc gaccgtcgtc
     3961 ggcatggtcg gtgcaggcgg gatcggcgtc accctgtggg aagcgattcg cggttttcag
     4021 ttccaacaaa cctgcgccct gatggtgctt atcatcgtca cggtcagcct gctggatttc
     4081 ctctctcaac ggttgcgtaa gcactttatc tgataagcga ggcattgata tctatgcact
     4141 tgtctacaca tccgaccagc tacccaacac gctatcagga gatagccgca aaacttgagc
     4201 aggagcttcg tcaacactat cgctgcggcg actatcttcc cgccgagcag cagctggcgg
     4261 cgcgctttga ggtaaatcgc cacaccctgc gccgcgccat cgaccaactg gtggaaaaag
     4321 gctgggtaca gcgccgtcag ggcgtcggcg tgctggtgct gatgcgcccg ttcgattacc
     4381 cgctcaacgc ccaggcgcgt tttagccaga acctgctgga tcagggcagc catcccacca
     4441 gcgaaaaact gctgtcggtg ctgcgcccgg catctggtca cgtcgctgac gcgctgggga
     4501 taaccgaggg ggagaacgtc atccacctgc gcaccctgcg ccgggtcaac ggcatcgcgc
     4561 tctgtttaat cgaccactac tttgcggacc tcaccctttg gccgacgctg caacgcttcg
     4621 acagcggctc gctgcacgat tttctgcgcg agcaaaccgg gattgcgctg cgccgcagcc
     4681 agacgcggat cagcgcccgc cgcgcccagg ccaaagagtg ccagcgtctt gaaatcccga
     4741 atatgtcgcc gctgctgtgc gtgcgcaccc ttaaccaccg tgacggtgaa agcagcccgg
     4801 cggagtactc cgtcagcctg acgcgcgccg acatgattga attcactatg gagcactgaa
     4861 tgcacgcaga tatcgcgacc cgccagcact ggatgtccat gctggcgcac agccaaccgg
     4921 ctgaactggc agcccgcctg aacgcgctga acatcaccgc caactatgaa gtgatccgca
     4981 ccgccgaaac cggcctggta cagattcagc cgcgaatggg cggtaccggc gaacgctttt
     5041 ttgccggcga cgccacgctg acccgcgccg ccgtacgcct gactgacggc cccctcggct
     5101 acagctgggt gctggggcgc gacaaacagc acgccgaacg ctgcgcgctg attgacgcgt
     5161 tgatgcagca atctcgccat tttcaaaact tatcagaaac ccttattgcc ccactggacg
     5221 ctgaccgtat ggcacgcatt gccgcacgcc aggccgaagt gaacgccagc cgggtcgact
     5281 tctttacgat ggttcgcgga gacaacgcat gaccctggaa accgctttta tgcttcctgt
     5341 gcaggatgcc cagcacagtt ttcgtcgcct gttaaaggcc atgagcgagc cgggcgtgat
     5401 tgtcgccctg catcagctca aacgcggctg gcagccgctg aatatcgcca ccaccagcgt
     5461 gctgctgacg ctggctgata acgacacgcc ggtgtggctt tccgcgccat taagtaacga
     5521 tatcgtcagc cagagcctgc gttttcatac caacgcgccg ctggtcaatc agtcggaact
     5581 ggcgaccttc gcggtaacgg atgaggcgat ttccagcgaa cagctcaacg ctctttccag
     5641 cggcaccgct gttgcgccgg aagcgggcgc gacgctgatt ttacaggtcg tcagcctgag
     5701 cggcgggcgc atgctgcgcc ttactggcgc gggtattgcc gaagaacgaa tgatcgctcc
     5761 gcagctgccg gagtgcattc tgcacgaact caccgagcgc ccgcacccgt tcccgctcgg
     5821 catcgacctg atcctgacct gcggcgagcg gctgctggct atcccgcgaa ccactcatgt
     5881 ggaggtgtgc tgatgtacgt tgccgtgaaa gggggcgaga aggagatcga cgccgcccac
     5941 gccctgcaag agagccgacg ccggggcaat accgatttgc ctgaactgag cgtcgcccag
     6001 attgaacagc agcttaatct tgcagtagat cgcgtgatga ccgaaggcgg cattgccgac
     6061 cgcgaactgg cggcgctggc gctgaaacag gccagcggcg ataacgtcga agcgattttc
     6121 ctgctacgcg cctaccgcac cacgctggcg aagctggcgg taagcgagcc actcgacacc
     6181 acggagatgc gtctcgaacg gcgtatttca gcggtttata aagatattcc cggcggtcag
     6241 ttgcttggcc caacctacga ctacacccat cgcctgctcg atttcaccct gctggcaaac
     6301 ggcgaagcgc cgacgctgac caccgcagac agcgaacaat ccccgtcgcc gcacgttttc
     6361 agcctgctgg cgcgtcaggg gctggcgaag tttgaagagg atagcggcgc acagccggat
     6421 gacatcaccc gcactccgcc agtttacccc tgttcacgtt cttcccgttt gcagcagttg
     6481 atgcgcggcg acgaaggcta tttgctggcg ctggcctact ctacccagcg cggttacgga
     6541 cgcaatcacc cgttcgcggg cgaaatccgc agtggttaca tcgacgtgtc gattatgccg
     6601 gaagagctgg gatttgcggt gaacgtcggc gaactgctga tgaccgagtg tgaaatggtc
     6661 aacggtttta ttgacccgcc ggatgagccg ccgcacttca cgcgcggcta cgggctggtg
     6721 ttcggcatga gcgagcgcaa agcgatggcg ctggttgacc gtgcgttgca ggctccggag
     6781 tacggcgagc acgcgacagg cccggcgcag gatgaagagt tcgtgctggc acatgccgac
     6841 aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc cccactacgt cgatttccag
     6901 gccgaactgg agctactcaa acgtctgcaa caggagcaga accatggcta atctgagcgg
     6961 ctacaacttt gcctacctcg acgagcagac caaacgcatg atccgccgcg ccatcttaaa
     7021 agcggtggcg atccccggtt atcaggtgcc gtttggcggg cgcgagatgc cgatgcccta
     7081 cggctgggga accggcggca ttcagctcac cgccagcgtg attggcgaaa gcgacgtgct
     7141 gaaggtgatt gaccagggcg cagacgacac caccaacgcc gtgtcgattc gcaacttctt
     7201 taagcgcgtg accggggtaa acaccactga acgtacggac gatgcgacgc ttatccagac
     7261 gcgccatcgc atccccgaaa cgccgctgac cgaagatcag atcattatct tccaggtgcc
     7321 gatccccgaa ccgctgcgct ttatcgagcc gcgcgaaacg gaaacccgca ccatgcacgc
     7381 gctggaagag tacggcgtga tgcaggtgaa actgtatgaa gatatcgccc gcttcggtca
     7441 tatcgccact acctacgcct atccggtgaa ggtgaacggg cgctacgtga tggacccgtc
     7501 gccgatcccg aaattcgata acccaaaaat ggacatgatg cccgccctgc aactgttcgg
     7561 cgcggggcgc gagaagcgca tctatgcggt gccgccgttt acccgcgtgg aaagtctcga
     7621 tttcgacgat cacccgttca ccgttcagca gtgggatgag ccatgcgcca tctgcggatc
     7681 gacccacagc tatcttgatg aagtggtgct ggatgacgcc ggaaaccgca tgtttgtctg
     7741 ctccgatacc gattattgcc gccaacagag cgaggcaaaa aaccaatgaa tcaaccgtta
     7801 ctttcggtca ataacctgac ccacctttac gcgccgggca aaggctttag cgatgtctct
     7861 tttgatttat ggccggggga agtgctgggc attgtcgggg agtccggctc cggaaagacc
     7921 acgctgctga aatcaatctc cgcgcgcctg acgccgcagc agggggagat tcactatgag
     7981 agccgttcgc tgtacgcaat gagcgaggcc gaccgccgtc gcctgctgcg caccgaaggg
     8041 ggcgtagtgc atcagcatcc gctcgacggc ctgcgccgcc aggtgtcggc aggcggcaat
     8101 atcggcgagc ggctgatggc gaccggggca cgtcattacg gcgatattcg tgccaccgcg
     8161 cagaagtggc tggaagaggt ggaaattccc gccaaccgga tcgacgacct gccgaccacc
     8221 ttttccggcg gcatgcagca acgtttgcag atcgcccgca acctggtgac gcatccgaag
     8281 ctggtgttta tggacgagcc gaccggcgga ctggatgtgt cggtacaggc acgcctgctc
     8341 gacctgctgc gcggcctggt ggtggagctg aacctcgcgg tagtgattgt cacccacgat
     8401 ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga tgaagcaggg gcaagtggtg
     8461 gagagtgggt taaccgaccg cgtgctcgac gacccgcacc atccgtatac acaactgctg
     8521 gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg gcgtaaacgc cttatccggc
     8581 ctacaaatgc gctccccgta ggtcggataa gacgcgtcag cgtcgcatcc gacacccgaa
     8641 ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa aaccttcatc ctgcatcagc
     8701 aaaacggcgt acgcctgccc gtcttacaac gtgcctcgct caccgtcaac gcaggcgaat
     8761 gcgtggtgct ccacggtcac tccggcagcg gcaaatcaac cctgctgcgc tcgctgtacg
     8821 ccaactatct acccgacgaa ggtcaaatcc agatcaaaca cggtgacgag tgggtagacc
     8881 tggtcaccgc gccagcgcgc aaagtggtgg aaatccgcaa aaccacagtc ggctgggtga
     8941 gccagtttct gcgcgtcatc ccgcgtatct cagcactgga agtggtgatg cagccgctgc
     9001 tcgataccgg cgttccgcgt gaagcctgcg ccgctaaagc cgcgcgtctt ctcacccgcc
     9061 tgaacgtgcc ggaacgcctg tggcacctgg caccatcgac cttctccggc ggcgaacagc
     9121 agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc cattctgctg ctcgacgaac
     9181 ctaccgcctc gctggacgcc aaaaacagcg ccgcggtggt ggaactgatt cacgaagcca
     9241 aagcccgtgg cgcagccatc gtcggcatct tccatgacga agctgtacgt aatgacgtcg
     9301 ccgaccgcct gcacccaatg ggagcctctt catgattatc aataacgtta agctggtgct
     9361 ggaaaacgag gtggtgcacg gttcgctgga ggtgcaggat ggcgaaatcc gcgcctttgc
     9421 cgaaagccag agccgcctgc cagaagcgat ggacggcgaa ggtggctggc tactacccgg
     9481 cctgattgag ctgcataccg acaacctcga taaattcttc accccgcgcc cgaaagtcga
     9541 ctggcccgcc cattcggcga tgagcagcca cgacgcgctg atggtggcaa gcggcatcac
     9601 caccgtgctg aacgcggtgg cgattggcga cgtgcgcgac ggcggcgatc agctggagaa
     9661 tctggagaag atgatcaaca ccatcgaaga gacgcagaaa cgcggcgtca accgcgccga
     9721 gcaccgtctg cacctgcgct gcgagctgcc gcatcacacc acactgccgc tgtttgaaaa
     9781 actggttcag cgcgaaccgg tgacgctggt gtcgctaatg gaccactcac cgggccagcg
     9841 ccagttcgct aaccgcgaga agtatcgcga atattatcag ggcaaatact ccctcaccga
     9901 tgcgcagatg cagcagtacg aagaagagca attggcgctc gccgcacgct ggtcgcagcc
     9961 gaatcgcgaa tccatcgccg ccctgtgccg cgcgcgacaa attgcgctcg ccagccacga
    10021 tgacgccacg cacgcccacg ttgccgaatc ccaccaactt ggcagcgtga tcgccgaatt
    10081 taccaccacg ttcgaagcgg cggaagcctc gcgtaagcat ggcatgaacg tgctgatggg
    10141 tgcgccaaat atcgtgcgtg gcggctcgca ctccggcaat gtggcggcca gtgaactggc
    10201 gcagcttggt ctgctggata tcctctcttc cgactactac cccgccagcc tgctggatgc
    10261 ggcgttccgc gtcgccgatg acgagagcaa ccgctttacg ctaccgcagg cggtgaggct
    10321 ggtgaccaaa aatccggcgc aggcgctgaa tcttcaggat cgcggggtga ttggcgaggg
    10381 taaacgcgct gacctggtgc tggcgcatcg ccagggcaat cacattcata tcgaccacgt
    10441 ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac tgatttggtt aatggggccg
    10501 tccggctccg ggaaagacag cctgctggcg gaactccgcc tgcgggaaca aacgcagtta
    10561 ctggtggcgc atcgctacat cacgcgcgac gccagcgccg gaagtgaaaa ccatatcgcc
    10621 ctgagtgagc gggagttttt tacccgcgcg gggcaaaacc tgctggcctt aagctggcac
    10681 gctaacggtc tgtattatgg cgtcggcatc gagattgacc tctggctgca cgctggattc
    10741 gacgtagtcg tcaacggttc acgcgcgcat ttgccgcagg cacgggcgcg ctatcaatcg
    10801 gcgctgctgc ccgtctgttt acaggtttcg ccggagatcc tgcgccagcg cctgaaaaac
    10861 cgtggccgtg aaaacaccag tgaaattaac gcccggctgg cgcgcgccgc ccgctatact
    10921 ccacaggatt gccatacgct caacaatgac ggcagcctgc gccagtcggt cgacacactg
    10981 ctgacgctga ttcatcagaa ggagaaacac catgcctgct tgtgagcttc gcccggccac
    11041 gcagtacgat accgacgcgg tttacgcgct gatttgtgag ctaaaacagg cggagtttga
    11101 ccaccacgcg tttcgcgtgg gctttaacgc caacctgcgc gacccgagca tgcgctaccc
    11161 gagcatgcgc taccatctgg cgctgctgga tggcgaagtt gtcggcatga tcggcctgca
    11221 tttgcagttt catctgcatc atgtcaactg gatcggcgaa attcaggaac tggtggtgat
    11281 gccgcaggcg tgcggtctga acgtcagcag taagttactg gcgtgggcag aagaagaagc
    11341 ccgccaggcc ggggccgaaa tgaccgaact ttccaccaac gtgaagcgcc acgacgcgca
    11401 ccgtttttac ctgcgtgaag gctacgagca gagccacttc cgcttcacca aggcgctgta
    11461 acatgagcct gaccctcacg ctcaccggca ccggcggcgc acagggcgtt ccggcatggg
    11521 gctgcgagtg cgtagcctgc gccagagcgc ggcgctcacc gcagtatcgc cgccagccat
    11581 gcagcggcgt agtgaagttt aacgacgcaa tcaccctgat cgacgccggg ctgcacgatc
    11641 tcgccgatcg ctggccgccc ggatcgttcc agcagttttt gctgacgcac taccatatgg
    11701 atcacgtgca ggggctattc ccgctgcgct ggggcgtggg cgatccgatc ccggtttacg
    11761 gcccgccgga tgaacagggc tgcgacgatc tgtttaaaca tccgggcctg cttgatttca
    11821 gccacacggt ggaaccgttt gtggtgtttg atttgcaggg gttacaggtc acgcccctgc
    11881 cgctcaacca ctcaaaactg accttcggtt atctgctgga aacggcacac agccgggtgg
    11941 cgtggctgtc tgacaccgca ggcttgccgg aaaaaacgct gaaattttta cgcaataatc
    12001 agccgcaggt aatggtgatt gattgcagcc acccgccgcg cgaggatgcg ccacgtagtc
    12061 actgtgattt aaacaccgtg ctggcgctga atcaggttat ccgctcgcca cgggtgattc
    12121 tgacccatat cagccaccag tttgatgcgt ggctgatgga aaacgcactg ccgtcggggt
    12181 ttgaggcggg atttgatggg atggagattg gggtggcgtg atgagaggga atgtgcgcgc
    12241 tggccccctc accctaaccc tctccccaga ggggcgaggg gaccgattgt gctcgatatt
    12301 gaatattgcg ctggttttct ccctctcccc attggggtga ggggcgatgc ctgctccata
    12361 cccaacctca tcgcccatac tcatcttcca ttctccgctc ttcatcttct agctgccgac
    12421 gctcctgatc aagctggcgc tggcgatcgt ccagctgcct gcggcgatct tcaaactggc
    12481 ggcggcggtc gtcatattgt ctgcgccgat cgtcgctcac ttcacgctgc cagccgtcgt
    12541 cgcgcgaatc ttcatagtct cgcccacggt cagggttata agcgtcgtta atcgcctgct
    12601 gaatattgcc aatggtgtcg tcgataatat cggcctgggc cggaacggtg gacagcgtga
    12661 gcagggtgaa taaaagaaat agcggaaagc gtttcattag ccaacctcaa aaagaaactc
    12721 tatccacatt aatcattact catccatgca agtagtggat gaatctcaat ttctccgctg
    12781 ctctattgcc gtaatcgcct ccacgcgttg ttgatgacga ccgccttcgt actgtgcgcc
    12841 cagccacgca tccacaatca tttttgccag ttcgaggcca accactcgtg aaccaaaagc
    12901 cagtgcaact tcactggcat aatgcggata atcagtgcgc tctgacgacc tggtgtcttt
    12961 atcaatcacc tcactcattg accatgaata aaaggctggc acagcaacgc agaatcctgc
    13021 ggtgccagcg acatgaccgc ccattgctcc ggtacgtttt ttaaaaagaa acaccggttg
    13081 cattgataaa ttgtgctcag tatttgccga agtacaatat atataataaa tatataaaac
    13141 acctcatatt atttacagag ataataaatt cgcgtaattt tctctcttca gcacaaatgc
    13201 ctaataaaat ggatcgttca gctcacactt ccacttatta aagtcgcaaa ttcccaatta
    13261 tgataacccc agctttaaat gacgtatttt gtatggatta catatttacg acataaatga
    13321 taaatggaga tttatatgaa taactctcgg ttattccgtt tgagcagaat tgttattgcg
    13381 ttaactgccg ccagcggcat gatggtaaat accgctaacg cgaaagagga agcgaaagcc
    13441 gccactcaat atacccaaca ggttaatcag aattacgcca aatcattacc gtttagcgat
    13501 cgtcaggatt ttgacgacgc tcagcgtgga tttatcgctc cgctgctgga tgaaggtatt
    13561 ctgcgcgatg ctaatggcaa accatatta
//
LOCUS       DAJTOR010000104        13585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTOR010000104 DAJTOR010000000
VERSION     DAJTOR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.104"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(242..1615)
                     /gene="cobA"
                     /locus_tag="OO249_002723"
     CDS             complement(242..1615)
                     /gene="cobA"
                     /locus_tag="OO249_002723"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5636964.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1634..2440)
                     /gene="nirC"
                     /locus_tag="OO249_002724"
     CDS             complement(1634..2440)
                     /gene="nirC"
                     /locus_tag="OO249_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5636965.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            complement(2567..2893)
                     /gene="nirD"
                     /locus_tag="OO249_002725"
     CDS             complement(2567..2893)
                     /gene="nirD"
                     /locus_tag="OO249_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5636966.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2890..5433)
                     /gene="nirB"
                     /locus_tag="OO249_002726"
     CDS             complement(2890..5433)
                     /gene="nirB"
                     /locus_tag="OO249_002726"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5636967.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRIFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5695..6876)
                     /gene="tsgA"
                     /locus_tag="OO249_002727"
     CDS             complement(5695..6876)
                     /gene="tsgA"
                     /locus_tag="OO249_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5636968.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7147..7719
                     /gene="ppiA"
                     /locus_tag="OO249_002728"
                     /gene_synonym="rotA"
     CDS             7147..7719
                     /gene="ppiA"
                     /locus_tag="OO249_002728"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5636969.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGAPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            7824..7991
                     /locus_tag="OO249_002729"
     CDS             7824..7991
                     /locus_tag="OO249_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5636970.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            7981..8583
                     /locus_tag="OO249_002730"
     CDS             7981..8583
                     /locus_tag="OO249_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5636971.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            8615..9178
                     /gene="pabA"
                     /locus_tag="OO249_002731"
     CDS             8615..9178
                     /gene="pabA"
                     /locus_tag="OO249_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5636972.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            9264..10484
                     /gene="argD/dapC"
                     /locus_tag="OO249_002732"
     CDS             9264..10484
                     /gene="argD/dapC"
                     /locus_tag="OO249_002732"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5636973.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(10551..12641)
                     /locus_tag="OO249_002733"
     CDS             complement(10551..12641)
                     /locus_tag="OO249_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5636974.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12692..13324)
                     /gene="crp"
                     /locus_tag="OO249_002734"
     CDS             complement(12692..13324)
                     /gene="crp"
                     /locus_tag="OO249_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5636975.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
BASE COUNT         3288 a         3494 c         3557 g         3246 t
ORIGIN      
        1 ttccctatgt attatatatt aattaatatt cattgattat atgaatataa gcagactgct
       61 cgttaagaac gatacaggaa tatttaattg gcgtcagtga gcgaatgact taattttgta
      121 agtcgctgta acggtgaata taacccatta gcagataata tttattaagg ggttatgttt
      181 tatttgctga atatataaaa agagtcatta agaaggccag aattctctgg cccgggttaa
      241 attaatggtt ggagaaccag ttcagtttat cgcgcaggcc aacaacccga ccaataataa
      301 ttagcgatgg actgttcatt tgctgagcaa gttcaccgag ttgcgtgagc gtaccgtcaa
      361 tcacgcgctg cgtgactgcc gttccgtttt cgacaattgc caccggcatt tcgccaggca
      421 taccgtgttc aatcagcttt tgctgaatag tcgcggcctg attcaacccc atatagaaca
      481 ccagcgtctg tttttctgcc gccaggtttt cccagtccag ctcgccaccg gtttttaagt
      541 gtccggtaat taagcgtacg ctctgggcat aatcgcgatg cgtgagtgga atacccgaat
      601 aggcagagca accagaagct gcggtaatac ccggaaccac cgagaacgga atacccgcgt
      661 tgcacagtgt ttccagctct tcgccaccac ggccaaaaat aaacggatcg ccacctttca
      721 gccgcaccac gcgtttgcct ttttgcgctt cccgcagcag gatctggtta atctcttcct
      781 gcggtacaca gtggtatccc gcgcgtttgc cgacgaaaac gcgatcagca tcgcggcgta
      841 ccagattcat aatatcgtca gaaaccagac ggtcgtagac caccacatct gcctgctgaa
      901 tttgttgcag tcctttcagc gtcagcagcc cggcatcgcc cggacctgca ccaaccagca
      961 ccacttcacc gcgatggtcg agcggttcgt tgattaactg ttcggtcgtt tcagtaatgg
     1021 ctttctgatc gttgtttgcc agcgactgcg ccaggcggtc gttaacgaac aatttctccc
     1081 agaaacggcg acgctcactc atcgtggtga actgttgttt cactcgcccg cgtaattgcc
     1141 cggcatattt cgccacctgg cccagatgca acggcagcag tgattcgagt ttttcgcgca
     1201 acaggcgcgc cagaaccgga gaggtgccgc cagaggagac cgctaccatt agcggtgagc
     1261 ggtcaataat cgacggcata ataaagctgg cggctttcgg cgcatcgact acgttacaga
     1321 agatgcgacg agcttcagcg gcttcgctga cgcgctggtt aagcgtgtca tcatccgtcg
     1381 ctgcaatcgc cagccagcag gtgtcgagaa ggctttcatc aaatggccct tcgacgaggg
     1441 ttaacatgcc tgcatctgcc catgcggtga actgtggaat aaacgctaat gcattcaccg
     1501 ttaagcgagc gcctgcgtct aacagcaacc ttgctttgcg ttccgcgaca tcaccaccgc
     1561 cgacaatcag acagtcgcga tcgcgtaatt ggcaaaatat aggcaaatga tccacgtaaa
     1621 aaccccttag taattaaccg gcagccgttt cagtttgatt aaatttgtcc gcaaccggac
     1681 gattcgattt cggcgtagca taccaataac ccaatcccat gaatacggca cctgataaag
     1741 tattacccag cgtcacccac agcaggttat gaccaatacc cgccagcgtg taggcttcgc
     1801 tgtggttgcc gaaccaggag agcgcgaaca gcgtcatgtt agcgatagag tgctcgtagc
     1861 cggacgcgat aaatgccagc agacaccacc agatagcgat aaatttcgcc gccccttcag
     1921 tgcgcagcgc catccagatt gccaggcaaa ccagccagtt acacaatgca cctttgaaga
     1981 agagtaccat tgccggtgca gtggttttag ccagcgcgac ggagtgaacg atgctggtat
     2041 ctaccggcag caggctaccg ccgccccagc tatagagcat ggcaacgaag acggaaccga
     2101 ccaggttacc cagccaggtt tgcggcagga ttgcccacat ttgcccgtgg ctgatgctgc
     2161 ccgcttttac cccaaaggtg aggaacatgg tgtgtccggt gaacagttca gaaccggcga
     2221 taatcaccag cgttaaggcg ataccaaagg tcgcgcccat caccagcgga cgtacggacg
     2281 ggtcgagcaa attaccgagc gtgaaaatca agatgatccc aagacccaca tacgcgcccg
     2341 ccatggcgga gctgacccaa aagccgagcg ggttatttgc cgacaggcgt gcaatgcgcg
     2401 cagcgttagc cgcacactta ttaatagtgt ctgtaaacat ttgattatcc tttaaaaaat
     2461 aaaaacaaaa aaataaaaca aaaagtttaa aaaatgtcct gcaatatacc gaaggtaatt
     2521 acaggaccat gcaaaaaggg gaggcattgc gcctcccgtt aaaacattaa ccgcgcagct
     2581 gcaccacgcc gtctttcact cgtgcttcgt aatgtttgac ggaaaactgt tcgtcttcca
     2641 tgcacaagcc atcacttaag cgaaaacgct gttttttcag cgggctggcg acccacagct
     2701 cgccctggtg ttccgcaatc agcccgcgtg acagcacgct ggactcgaag aacggatcga
     2761 tgttgctgat cgcaaacacc tggtcgctgt gatacgggcg gaaaatcgcg acctgctcgt
     2821 cgcctaacag cgcgcagacg ccggtttcag gcaggatgtc atcgattttg cagatgtctt
     2881 tccactggct catgcgttgt cctccaccag agttaccggg atacgttcat acggcgttgc
     2941 cggacggtgc tgttcgcgct ctggcaccat ctgcacgttc gggtcacgct tgtcgctgtt
     3001 gatgaagtgt ttgaagcgag tctgcgcgga cggtgtattg accgtttcag tccactcaca
     3061 cactaccgct tcacgcaggc gcgccatctc ttcttccaga tgagcgttca gccccagttt
     3121 gtcgtcaatg atcaccgctt tcaggtaatc gatgccgcct tcgaggtttt ctaaccacgg
     3181 cgcggtacgc gtcagtttgt cggcggtacg gatgtagaac atcatgaagc ggtcgagata
     3241 tttgatcagc gtttcgcgat cgatatcagc tgccagcaga tccgcatgac gcggtttcat
     3301 gccgccgttg ccgcaaacat acaggttcca gcctttttca gtggcgataa tacccacgtc
     3361 tttaccctga gcttctgaac attcacgggt acagccggag acaccgaact tcattttgtg
     3421 cggcgtacgg atgcctttgt agcggttttc cagttccacg ccgaggccga cgctgtcgcc
     3481 aacgccgtag cggcaccagg tgctacccac gcaggttttc gccatacgca gtgctttcgc
     3541 ataggcatga ccggtttcga agccggcttc aatcagctga cgccagatct ctggcagatc
     3601 gtctttctgt gcgccaaaca tcgccaggcg ctgggaaccg gtgattttgg tgtagagatt
     3661 aaattcacgc gcgatacgac ccactgccat tagcccttcc ggggtgattt caccgcccgg
     3721 agaacgcggg atcaccgagt aggtcccgtc tttctggatg ttagcgagga agttgtcgtt
     3781 agaatcttgc agcggagtat gttctggctt cagaatgtat tcgttccagc aggaggccag
     3841 cagcgaaccg acggtcggtt tacaaacttc acaaccgtag cctttgccgt gtttcgccag
     3901 cagttcttcg aaggttttaa tgccttcaac gcggatcaaa tggaacagtt cctgacgcga
     3961 ataagcaaag tgttcgcaca ggttgttgtt aacttcgatg ccctgtttcg ccagttccgc
     4021 gttcagtacc tgagtgacca gcgggataca gccaccgcag ccagtacccg ctttggtttc
     4081 agctttcagc gccgcaactg tgtggcagcc tttgttgatg gcagcaatca gatcaccttt
     4141 agtgacgtcg aagcaggagc agatttgcgc gctgtccggc agtttatcaa caccgataga
     4201 cggcttaccg ctacccgagt gcgccggcag aatcagagaa tccgggtttt ccggcagctc
     4261 gatagcattc agcaccagtt gcagcaagtt accgtagtcg ctggtatcgc ccaccagtac
     4321 cgcaccgagc agggttttgt tgtcttcgct gacgatcagg cgtttgtaga tctctttgct
     4381 ttcgtcgagg taaacgtagc tacgtgcgcc aggcgtgcga ccatgagcat caccaatacc
     4441 gcctacgtcc acgcccagca gtttcagctt ggcgctaagg tcagcacctt caaaggcgtt
     4501 ttcgctaccg agaatatggt caacggcgac ctgcgccatt ttgtagccag gtgctaccag
     4561 accaaatata cggttgttcc agcttgcgca ttcaccgata gcgtagatat ccggatcgga
     4621 agtttggcag gaatcattaa tgacgatacc cccacgcgga gcaacgtcca gaccacactg
     4681 ggttgccagc ttatcgcgcg gacggatacc ggtagagaag acgataaagt cgacttccag
     4741 ttcgctgccg tcggcaaaac gcatggtttt acgtgcttcg acaccttcct gcacaatctc
     4801 aagggtgttt ttgctggtgt gaacgcgcac gcccatactt tcgattttgc gacgcagctg
     4861 ctcgccaccc atctgatcaa gctgttccgc catcagcata ggggcaaatt cgataacgtg
     4921 ggtttcaata cctaagtttt tcagcgcgcc agcggcttcc agacctaaca ggccgccacc
     4981 aacaacggca ccgcgtttgc tgcgacgggc gcaggattca atggcgttga ggtcttcaat
     5041 ggtgcgatag acaaagcagt cctgagtatc agaacctttg attggtggga tccacgggta
     5101 ggaaccggtt gccatgatca gcttgtcata aaaaacggta cgtccggcgc tggagtgaat
     5161 caccttctcc tgacggttga tggtgatagc gcgttcgccg accagaactt tgatgccgtg
     5221 tttctcgtag aagccttcgc gcaccagcga cagctcttcg gcggtgtggt gagagaagta
     5281 agacgagagg tgtacgcggt cataagcgat gcgcggttct tcacagaaaa cggtaatatc
     5341 aaagttggcc gcatcagatt tatcaagaag atcttcgata aagcgatggc cgaccatacc
     5401 gttaccgata attgcgagtc tgactttgct catttttgcc tcgatttctt ttctattacc
     5461 gcctacctta acgattcagc aaccccgctt attgatgtaa atcaaattca cctttatata
     5521 ccccttaagg ggtatgttgc tgatttgtat aaattttctt aagtcacgga attgtctgac
     5581 atttcatgtt tgtgcaaaaa atagacaaaa tgaacgggtt tatagaaata gatgcacgta
     5641 gcggggttag agaggaaaag gccggacgac gatagtgccg tccggcatta gcaattaatg
     5701 agaggtcagg gtgttatgct gacggtgacg gctgacgaaa cctaacagga agcacatcac
     5761 aaagacgaca gcgtacagac cgtttgctgt aagcagtgcc gcctgcggac cgctatgttc
     5821 aacaatcggg ccggtaacca caaaggtcaa catagtaccg atggttccgc aggtcaggac
     5881 aaagttaacc agttttggcg acggtacttt ggtctgctgt gaacccaggg tgatgatggt
     5941 ggtatagatc gcgctggaga agaagcccag agcgagaatt gaccacgcca tatgtgctgg
     6001 tgttccggtg ttaaagacgt acatcagaat cgcagccaga ccagccagta cggtcagaat
     6061 gcgttgcaaa tcaaagaagc gaagaataaa gctgaacgcc cacatgccga ccatgtatga
     6121 catccagaag ttactcacca gcgtgcccgc gtcgttcagg ctcatgccca ggcctttggc
     6181 atactcaggc acccaggaga taaaacctaa ctgaccgagg atgtagcaca gcgccgcaac
     6241 agagagaaac agtacgccga tcccccactt ttctttctct accggagcat ccgtttttgg
     6301 tgcatgtttg cccagtgctg ggaactcaca gccgaaggtc agaataaaaa tagcgacata
     6361 caccagcccg atgcaggcat aaacccagta ccactcaatg ctgcgcgcca gtagaaacgc
     6421 ggcgatcatt gggaaaatca tcccagccat actgaagaag gagtcagtaa ataacaggcg
     6481 ggaaccgcgc tgacgccctt catacatttg tgttatcagg aatgtaccaa tcgacatagt
     6541 aatgccgctg accaccccga gaatgaacat cgccgtcgag aacagcgcca ggctgtggct
     6601 gaacatcaaa ccggcaaccg ccagcaccat caggagaaag ccaaaacgta actgcgtttt
     6661 caacgggacg atttccatca gccaggcgtt gaggaagata gagattaaaa tgccggcgtt
     6721 gaggaaggtg aaggtgttac tcatactgga aacaggcaga ttgaaataat cggcgatatt
     6781 tcccatcacc atcccggtga caataaccaa cgcaccagtc agtgcgtagg agagaaagct
     6841 aatccatgtg agcttgatgc gattgctgtt agtcatgatt ggcctgcgtt caaaaataaa
     6901 atggcatagc gggatatgcc gcgagcgggt gattttaggt gattttgtga tctgtttaaa
     6961 tgttttattg caatcggttg ctaaattgca ttttaagaag tgatcttgat cacggaataa
     7021 aaagtgatcg tcaggttaca tatatttcag atacgtaaaa ttaggtaaag ggatggcctt
     7081 gttcttgaag gctatttaga atctcttcac ttgctttttt tctgctctgt ttgttaagga
     7141 aatctcatgt tcaaatcgac cctggcggcg atggctgctg ttttcgctct ttctgctctt
     7201 tctcccgcag caatggcagc gaaaggggcc ccgcacgtat tgttgacaac ctcagctggt
     7261 aacatcgaac tggagctgga taaacaaaaa gcgccagtgt ctgtgcaaaa ctttgtcgat
     7321 tatgtgaaca gcggttttta taacaacact acctttcacc gcgtcattcc tggctttatg
     7381 attcagggcg gcggtttcac cgagcagatg cagcagaaaa aaccaaaccc gccaatcaaa
     7441 aatgaagccg ataacggcct gcgcaacacg cgtggcacca tcgcgatggc gcgtaccgct
     7501 gacaaagata gcgccaccag ccagttcttt atcaacgttg ccgataacgc cttccttgac
     7561 catggtcagc gtgatttcgg ttacgcggta tttggtaaag tggtgaaagg catggacgtg
     7621 gccgataaga tttcccaggt gccgactcat gacgttggtc cgtaccagaa tgtgccgtca
     7681 aaaccggtag ttatcctttc cgctaaagtc ctgccgtaat gatttctcgc gcgggcaatc
     7741 ttgcccgcgc ttctgctctc ccggcgtaac ccggatttgc cgcttatact tgtggcaaat
     7801 ggacacgttc agggaggcat caagtgaaga aactcaccga taagcaaaag tcccgtctct
     7861 gggagcttca gcgtaatcgt aatttccagg ccagtcgccg tcttgaaggc gtcgagatgc
     7921 ctttagtcac tcttactgcc gcagaggctt tagcgcgcct tgtagagctg aggaggcact
     7981 atgagcgata aattcggcga agggcgcgat ccgtatcttt atccaggcct tgatatcatg
     8041 cgtaaccggc tgaacatccg ccagcaacaa cggctggaac aggccgctta cgaaatgacg
     8101 gcgctgcgtg ctgcgaccat tgagcttggt ccgctggtgc gcggtttacc gcatttgcga
     8161 actatccatc gccagctgta tcaggatatt ttcgactggg cagggcaact gcgtgaagtt
     8221 gatatttatc agggcgatac gccgttctgc cactttgctt atatagaaaa agagggcaat
     8281 gccctgatgc aggatctgga ggaagaaggt tatctggttg gcctggagaa aacgaagttc
     8341 gtcgagcggc tggcgcatta ctattgtgaa atcaacgtgc tgcatccctt ccgggtggga
     8401 agtggtctgg cacagcggat cttcttcgag caactggcga ttcatgccgg atatcaactg
     8461 agctggcagg gtatcgaaaa agaggcctgg aatcaggcaa atcagagtgg ggcaatgggg
     8521 gatctcaccg cactgcagat gatatttagc aaagtggtaa gcgaagccgg ggaatctgag
     8581 taaaatagcg cggttctttc gtaccggagc cgccatgatc ctgcttattg ataactacga
     8641 ttcttttacc tggaacctct accagtactt ttgtgaactg ggggcggatg tgctggttaa
     8701 gcgcaacgat gcgttgacgt tggcggatat cgacgccctt aaaccacaaa aaattgtcat
     8761 ctcacccggc ccctgtacgc cagatgaagc cgggatctcc cttgacgtta ttcgccacta
     8821 tgccgggcgc ttgccgattc ttggcgtctg cctcggtcat caggcaatgg cgcaggcatt
     8881 tggcggtaaa gttgtgcgcg ccgcaaaggt catgcacggc aaaacctcgc cgattacaca
     8941 taacggtgag ggcgtatttc gggggctggc aaatccactt accgtgacac gctaccattc
     9001 gctggtggtg gaacctggct cattaccagc gtgctttgac gtgacggcct ggagcgaaac
     9061 ccgcgagatt atggggattc gccatcgcca gtgggatctg gaaggtgtgc agttccatcc
     9121 agaaagtatt cttagcgaac aaggacatca actgctggct aatttcctgc atctctgatt
     9181 tccgattgcc attcagtgat tttttatgca tattttgtga ttataatttc atatttattt
     9241 atgcgtaaca gggtgatcat gagatggcaa ttgaacaaac agcaattaca cgcgcgactt
     9301 tcgatgaagt gatcctgccg atttatgctc cggcagagtt tattccggta aaaggtcagg
     9361 gcagccgaat ctgggatcag caaggcaagg agtatgtcga tttcgcgggt ggcattgcag
     9421 ttacggcgtt gggccattgc catcctgcgc tggtgaacgc gttaaaaacc cagggcgaaa
     9481 ctctgtggca tatcagtaac gttttcacca atgaaccggc gctgcgtctt gggcgcaaac
     9541 tgattgaggc gacgtttgcc gaacgcgtgg tgtttatgaa ctccggcacg gaagctaacg
     9601 aaaccgcctt taaactggca cgccattacg cctgtgtgcg tcatagcccg ttcaaaacca
     9661 aaattattgc cttccataac gcttttcatg gtcgctcgct gtttaccgtt tcggtgggtg
     9721 ggcagccaaa atattccgac ggctttgggc cgaaaccggc agacatcatc cacgttccct
     9781 ttaacgatct ccatgcagtg aaagcggtga tggatgatca cacctgtgcg gtggtggttg
     9841 agccgatcca gggcgagggc ggtgtgacgg catcgacgcc agagtttttg cagggcttgc
     9901 gcgagctgtg cgatcaacat caggcattat tggtgtttga tgaagtgcag tgcgggatgg
     9961 ggcggactgg cgatttgttt gcttacatgt actacggtgt gacgccggat attctgactt
    10021 ctgcgaaagc gttaggcggc ggcttcccga ttagcgccat gctgaccacg gcggaaattg
    10081 cttctgcgtt tcatcctggt tctcacggtt ccacctacgg cggtaatcct ctggcctgtg
    10141 cagtagcggg cgcggcgttt gatatcatca atacccctga agtgctggaa ggcattcagg
    10201 cgaaacgcca gcgttttgtt gaccatttgc agaagatcga tcagcagtac gatgtgttta
    10261 gcgatattcg cggtatgggg ctgttaattg gcgcggagct gaaaccacac tacaaaggtc
    10321 gggcgcgtga tttcctgtat gcgggtgcag aggttggcgt aatggtgctg aatgccggac
    10381 cggatgtgat gcgtttagca ccgtcgctgg tggtggaaga tgcggatatc gatgaaggga
    10441 tgcaacgttt cgcccacgcg gtggcgaagg tggttggggc gtaaataaga cgcatcaggc
    10501 aaaatatgtc ggatgcggcg tgaatgcctt atccgatggt ttagcctgta ttacgccttc
    10561 gaatcccgca acttgcgact cagccaaatc ccgtgatgcg gtcgctgacg ccatgccatc
    10621 gacgaaatgg tgtgcatggt gttcagatga ccaataaccc gctgtaaatg ctgctccagc
    10681 gtacctgccg cgccttcgtg cggctgcatc tccggcgcat ccatgatatt ggcatcgcca
    10741 gaactacccg gttcgtcata ctccagtcgc tgctgacaac gctgaatggc gatttcacaa
    10801 gactgtaaat actcttgtgc cagttcaggt ggcaatgccc ggtgttcccg cgccagcgtg
    10861 gtcatggcat taatatgctc aacaataaac tggctgtgcg ttacccacag tttcatatct
    10921 gccagataat ggctgttaaa cgccggttcc tgcatcgcct gattcaatga gttatacaga
    10981 gtgttatgtg cctgatttac ccgcattcgc tgccaggcca gtggcgtagg ttgcggatcc
    11041 tcgctaagaa tcaagcgaat cgcttcctga taggcttcta aagcgtcatg ggcgttttta
    11101 cgcaataacc cgctctgcca ctgcggccac agccagacag taccgccgaa agcaattaaa
    11161 caaccaataa tggtatcgat aagacgcgga aggatgtatt gctcgccgtt caaccacaat
    11221 agttgcaggg tatacactgc ggtaatagta aaaccgaccg tcgcccagcc gtagtttttg
    11281 cgcaatatca ggtagctggc gagggtggta atcagcatca acgtcagggt gtaaccttcg
    11341 ggaattttaa agtgcagcgc cacgcccgca atgattaacc cgaccacggt tcccacggag
    11401 cgattcacaa tcctcagacg ggttgcgcca tagccatttt gtgtcaccag caataccgtc
    11461 atcaggatcc agtacgactt cggcagatgc agcgcggtgc ccatcaggct ggcaacgctt
    11521 aacatcacac tgagtcgtcc ggcattgcgt agcgccggag actttagtga cagataactt
    11581 ttcagcgcag gaagtaatgg cattcgccgc tgtttatcgg ccagtaagtc acgggcatag
    11641 agcggttttt gggtgcgcag cacgcgggcg atgcggctga aatgccagta gcagaattgc
    11701 ccaaccggat tatccggatg ctggcgggcg attttttcca gtgcgccaat ttgcttttcc
    11761 atcgtaaaac gcgttggcag gcgatggtaa agaatgtcat cagccagcac gcgcaggcga
    11821 gcggcgacgg tttgcgcatt ccagcggata acttcttccg catggctacg ctcgaccagc
    11881 ttttgcacct cttccggctg atgcaaactg accgaaatat gttcctgtaa atccagcgcc
    11941 tcctggaaaa tacgcagcat ccgcttgtag tcagtattat tttgcgcgga aagcatatgc
    12001 atttgctgat agcactgggt aattagatcg accgcttttt gctggcgcac cagcagcggc
    12061 ggcagcgctt tttcagggtc ggtgtgctgg gtaagcaggc tgtatttggc ttcacaataa
    12121 tctgccagtt cacggtacag cagacttagt gactcgcgca gcggttgttc gcgccagatc
    12181 cagaaccaaa accagttaaa caatccgtac cagagagtgc ccaacgcata gatgagcaac
    12241 ggttcccaga ccggcatgta tcccgccaga ctgagggtaa aaatggctgc cagcagcgat
    12301 gcgggaagca attttgcgtg caatggcccc aactcagcag tgacgccaag taccagcgtt
    12361 aatccggtca gcaaaaaggg caggggaaca tcttttgcca gtagcagctg tgtcagtaag
    12421 ctacaggtgg caaacagcga cgcaccaatg attaagcgtt tgaaaaaacg tttatgaggc
    12481 gtatcaaggc ccgcaatatt gcaacaggca ggaacgagag agaagagcag accgaatcgt
    12541 aattcgccaa gcattaaccc aacggccacg ggcaaacata gcaccagcgt ttgtcgaagt
    12601 gcatagttga tatcggggtg ataaatcagt ctgcgccaca tcgggggaaa caaaatggcg
    12661 cgctaccagg taacgcgcca ctccgacggg attaacgagt gccgtaaacg acgatggttt
    12721 taccgtgtgc ggagatcagg ttctgatctt caagcatctt cagaatgcgt cccacggttt
    12781 cacgagaaca gccgacaatc tgaccgattt cctgacgggt aattttgatt tgcataccgt
    12841 ccgggtgagt catagcgtct ggttgttttg ccagattcag cagagtctgt gcaatgcggc
    12901 ccgtcacgtc gaggaacgcc aggttgccca ctttctctga agtgacttgc agacgacgcg
    12961 ccatctgtgc agacagacgc atcagaatgt ccgggtttac ctgaatcaat tggcgaaatt
    13021 ttttgtacga aatttcagcc acttcacagg cggttttcgc acgtacccat gcgctacgtt
    13081 cctggccctc ttcaaacagg cccagttcgc caataaaatc accctgattc agataggaga
    13141 ggatcatttc tttaccctct tcgtctttga tcagcactgc cacagagcct ttaacgatgt
    13201 agtacagcgt ttccgctttt tcaccctggt gaataagcgt gctcttggat gggtacttat
    13261 gaatgtggca atgagacaag aaccattcga gagtcgggtc tgtttgcggt ttgccaagca
    13321 ccatgcgcgg ttatcctctg ttataagctt tctccagagc cagataacgc cgctgtctct
    13381 ggattgccga aatatgcttc ccgctacctg ggaaggggct atcaactgta ctgcacggta
    13441 atgtgacgtc ctttgcatac atgcagtaca tcaatgtatt actgtagcat cctgactgtt
    13501 ttagcatagc tttcgctttg tgtctcctgg tgtctcgctt cagcatgacc caggtcgcct
    13561 tccgttgcgc gatttggtta gtacg
//
LOCUS       DAJTOR010000105        13370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTOR010000105 DAJTOR010000000
VERSION     DAJTOR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.105"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..1862)
                     /gene="speC"
                     /locus_tag="OO249_002735"
                     /pseudo
     CDS             complement(1..1862)
                     /gene="speC"
                     /locus_tag="OO249_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            2260..2958
                     /locus_tag="OO249_002736"
     CDS             2260..2958
                     /locus_tag="OO249_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5636976.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            3064..3139
                     /locus_tag="OO249_002737"
     tRNA            3064..3139
                     /locus_tag="OO249_002737"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3097..3099,aa:Phe,seq:gaa)
     gene            complement(3288..3824)
                     /gene="yghD"
                     /locus_tag="OO249_002738"
     CDS             complement(3288..3824)
                     /gene="yghD"
                     /locus_tag="OO249_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HCR5636977.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(3826..5004)
                     /gene="gspL"
                     /locus_tag="OO249_002739"
     CDS             complement(3826..5004)
                     /gene="gspL"
                     /locus_tag="OO249_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCR5636978.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5001..5978)
                     /gene="gspK"
                     /locus_tag="OO249_002740"
     CDS             complement(5001..5978)
                     /gene="gspK"
                     /locus_tag="OO249_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCR5636979.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5981..6580)
                     /gene="gspJ"
                     /locus_tag="OO249_002741"
     CDS             complement(5981..6580)
                     /gene="gspJ"
                     /locus_tag="OO249_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCR5636980.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6577..6948)
                     /gene="gspI"
                     /locus_tag="OO249_002742"
     CDS             complement(6577..6948)
                     /gene="gspI"
                     /locus_tag="OO249_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCR5636981.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7008..7508)
                     /gene="gspH"
                     /locus_tag="OO249_002743"
     CDS             complement(7008..7508)
                     /gene="gspH"
                     /locus_tag="OO249_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCR5636982.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNAC"
     gene            complement(7512..7967)
                     /gene="gspG"
                     /locus_tag="OO249_002744"
     CDS             complement(7512..7967)
                     /gene="gspG"
                     /locus_tag="OO249_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCR5636983.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLARTVVPNLLGNKEKA
                     NRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7984..9207)
                     /gene="gspF"
                     /locus_tag="OO249_002745"
     CDS             complement(7984..9207)
                     /gene="gspF"
                     /locus_tag="OO249_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCR5636984.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAM
                     SDALQASGVYWLAGLLALLVLGQWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYIIASGEQRGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9207..10699)
                     /gene="gspE"
                     /locus_tag="OO249_002746"
                     /pseudo
     CDS             complement(9207..10699)
                     /gene="gspE"
                     /locus_tag="OO249_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10699..12759)
                     /gene="gspD"
                     /locus_tag="OO249_002747"
                     /pseudo
     CDS             complement(10699..12759)
                     /gene="gspD"
                     /locus_tag="OO249_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12789..>13370)
                     /gene="gspC"
                     /locus_tag="OO249_002748"
     CDS             complement(12789..>13370)
                     /gene="gspC"
                     /locus_tag="OO249_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HCR5636985.1"
                     /translation="CGARGIAFGARPGAVIEEGGKQQVYLQGETLGSHNAVIEEINRD
                     HVMLRYQGKIERLSLAEEERSTVAATNKKAVSDEAKQAVAEPAVSAPVEIPTAVRQAL
                     AKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIALNQQDFTDPR
                     AMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
BASE COUNT         2987 a         3869 c         3448 g         3066 t
ORIGIN      
        1 tatgcgcatc ctgggggttc atcaccactg acgggaaact ctgctggcgg aacatcgctt
       61 tttgtaggtc tttgacgtcg aaactgacat acagatcgtg catctcctga cacaactggc
      121 gcaaagtgta gtcgcgatag cgcaccggat atttgttata aacgctcggc aacacctcag
      181 ccagcggcga gtcatcctca atatgctgtt caaactgcgc caacatctct accagttgtg
      241 ccagcttctc gtggctttcc gccggagtta ataagaacag aatggagttg agatcgcact
      301 tctccggcac aatgccgttc tcacgcagat agtgcgccag aatcgtcgcc ggaacgccaa
      361 agtcgctata ttcgccggtt tcggcattga tacctggtgt agtgagtaac agcttgcacg
      421 gatcaacaaa atactgatcc gcggcatatc cttcaaagcc gtgccacttc gcccccggct
      481 caaaactgaa aaaacggcgg tcgctggcta acactgatgt cggataatcc tgccacaatt
      541 tgccatcaac aacgggcggg ataaacgggc ggaacagctt acagcgcgca agaatagcct
      601 tgcgcgactc aatacccaac tcaacgcact cagcccacag ccgacgccca ctctcccctt
      661 catgaatttt ggcgttaaca tccagtgcag caaacagcgg atagaaaggg ctggtagaag
      721 catggagcat aaaggcgtta ttcaaccgct tatgcgggca aaaacgcgcc tgtccgcgga
      781 tatggttatc ttttttatgg atctgcgacg tctgtgagaa tcccgcctgc tgtttgtgca
      841 ccgactgagt cacaaagatc tccggatcgt tttcgttaag ttctaatagc agcggcgagc
      901 tatccgccat catcgggata aactgttcgt aaccgaccca cgcggaatca aacagaatgt
      961 aatcacacag atgcccaacg gtatcgatca cctgacgggc gttatagaca gttccgtcat
     1021 aggttcccag ctgaataatc gccaggcgaa acgggcgcgg cagctcggct ttttctggcg
     1081 caacgtcgcg aatttgctgg cgcagatact cttcattaaa acagtgcgca tcaataccgc
     1141 caatgaaacc aaacgggttg cgtgaagctt ccagatagac cggcgtcgcc cccgcctgaa
     1201 tcagcgcgcc gtgatgattc gacttatggt tgttacggtc gaagagcacc agatcgccac
     1261 gcgttaacag cgcattcgtc accactttat tcgctgccga tgtgccgttc agcacaaaat
     1321 aggttttatc ggcatgaaag actttggctg cgaatttctg cgcatctttc gccaataagc
     1381 agatcgccca attttacgtc agcgttacac atatcggcgc gaaagacgtt ctcaccaaag
     1441 aaatcgtaaa aatggcgtcc ggcaggatgc tttttaaaaa acgcaccatg ttgatgtcca
     1501 gggcaagcaa aggtgctgtt gcccatctca acgtactgcg tcagcgtgtc ataaaacggt
     1561 ggcagcaaat tctcttcata ctgacaggct gcggattcca gctccagcca ctgctgctcg
     1621 ttgccgttga ttaccgccgt aacgcccgca ggtaattcaa cagcatgttc ggaatacaaa
     1681 aacaccggta gatgaaaacc ggtgcgctta agcaacgcaa gaatgccact gcgactatcc
     1741 gcagcggtaa tgacgactgc cacgacgtcc gtaaaatcag tatctcccaa cgccaccacg
     1801 cgacgatgag aagaaagtcg ggataccagt tcactactgg cggcaatatt cattgatttc
     1861 ataagcgcaa acccgtttcg gggaagtaag aataccggac aaggtggaaa accctgcccc
     1921 atgagatatg ggtcaaactg gtcaccagct ccgaccgcca gacatcagta aaagcagaaa
     1981 cgctctgatt ttactgttgt cctgcagtga gcgtgcgtta acttcaccgc atgagcagta
     2041 acatagaaag ggaaacgttt cgcgcgaaac ggcgataagc gagagaatgt aaggaggtgg
     2101 cgtgcatcgg gcaaactccg tacagagagg agaaaattcg cgcaatcatg gcacctttcg
     2161 ctaaagcgtg caagccagga ctttgcgacc agacaagcca tcaacaaggc aaaaagttca
     2221 taataagaaa atcaaacaat acagttaaca ggaactcttg tggtcatcgg cccttttatc
     2281 aacgctagtg cagtcttact gggtggcgtt ctcggcgcac tgctcagcca acgcttaccg
     2341 gaacgtatcc gcatctccat gacatctatt tttggtctgg catcgctggg gattggtatt
     2401 ttactggttg tgaaatgcgc caaccttcca gcgatggttt tagctacgct acttggcgct
     2461 ctaattggcg aaatttgttt gctggagaaa ggtgtcaata cagcagtcgc caaagcacaa
     2521 aatctgtttc gtcactcacg taagaagcca acgcatgaat cttttattca gaattatgtc
     2581 gcgattattg tcctgttttg cgccagcggc accgggatct tcggggcgat gaacgaaggg
     2641 atgaccggcg atccgagtat tttaatcgcc aagtcatttc ttgatttctt tacggcgatg
     2701 atcttcgcct gctcgctggg tattgcggtg tcggtaatta gtatcccatt actgatcatc
     2761 cagttaacgc tggcgtgggc tgccgcgctg atattaccgc tgaccacacc gtcgatgatg
     2821 gcagacttca gcgccgtagg cggtttattg ctgctggcaa ccggattacg catctgtggc
     2881 attaaaatgt tcccggtggt caacatgctt ccagcactcc ttctggcaat gccgctttcc
     2941 gccgcctggt ttgcctgaca atgcgtgcaa tagcggcaaa gtgatgatag attgtgcagt
     3001 ctgcagtaaa ttgaagaaat ttgattgacg agacgaggcg aatcaggttt aatgcgcccc
     3061 gttgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg
     3121 attccgagtc cgggcaccac taattcttaa gaacccgccc acaaggcggg tttttgcttt
     3181 tggatctgac aataaccttc acgaaaaaag tttgcttata aagtctgggg gaattactct
     3241 cgccacgtta acgagagtaa ttttattgat actaatctcc tgatacttta cccccgtcca
     3301 aactccagcc gctggacatt caccatccca gtcttctcag cagcactggc atcaatttgt
     3361 gtcacccgca gcgcatactt ttcatccagc gcgtttaacc agttcagcag gtcattaaac
     3421 accaccggat ctatccagac ctgaatattc tccccacgct cggctatccg cttgatgact
     3481 accgagtgcg cggaagcact gtcactgata acccgcgata cctgcgcagg cgttgttgtg
     3541 gcggattttc gcgccgcaat aatatccggc gcggcgctcc tcagtctggt gttcatcgcc
     3601 accagttgct gcaacattgt ctcctgttgt actatccgtt cgctcaacgg ctgccagatg
     3661 aggacgtaat atccgacgct aaacaggaac accaccgctg ccagtaacat gcccttttca
     3721 cgcggagaac gccccgccag gtgttgtgcc agccagtgtt cgccacggct taactggcgt
     3781 tcacgccatt gctgaaaata gtgaataaat ttatcgcgta acatgttatt tcctccgcaa
     3841 cgttacgccg ccggaaaccg catcaccctc tttctgtaac gcgtcctgtt gcacaacata
     3901 atcggttgcc agtgcactac gcagtttgtc gaaactggca aagttcgcgg cccgtagctg
     3961 gaggtgaagt atctggcgtt tttgatcaaa ggtaaaacca cgcatttcga tgtcggaaag
     4021 tgacgctgat ttcagggtgc tggctatcgc tgacaactcg gcgagcagcc gggtatcgtc
     4081 ggcctgtggg cgatattttt tcagcgccat cgtcacctga gagcgtaaat tcacaatccg
     4141 cttctgctcc gggaacaacg ttaagaactg ttcctccgcc tgggtgcggc tttgcgccac
     4201 ctgttcgctg acgctccata acgtcacgcc ccgctccact gccagcgcaa ctagaatcag
     4261 caatatcggc agaatcatca cccgccagcg cgcccactgt tttcggtagc tgacgcgagg
     4321 ctgccacggc cctgtcagca ggttcccttc cggttcgcca taagtggtaa cggcgggcag
     4381 agctgtaacg gtcaggcgtt cagcgtctgc gccagcccat gctgatagct tttccggtgc
     4441 aatgccgact acggttagcg aaagcggtaa atcctgctca ttgagctggg cgcggaacat
     4501 gaccggagcc agcgcccgcc cggcgctcca tccccggcat tcatcaatgc ggcagataac
     4561 ccgttgcgca tcgccagcca taaccccaca aggaatggac atccagtctg gcgcgacggt
     4621 agcgcgggtg atgccgtttt cctgcaacca ctgcgcaatg ttgcgcatat gctgttggtg
     4681 aatcactgct acggttgcca gttgctggtc gatattcaac ggggcgaaat gcagttcatc
     4741 gatatcctgg ttcagctctt cttccagtaa ggcgggcaga atggtcggta tctgcttacg
     4801 ggcgacatca ggcagttcaa cctgccagac gctgatccat tcgccgggaa tgtagagtcg
     4861 aatcgcatcg gtttgcagcc attgctggag ctgttcgtta gcaacgtcag accagatacc
     4921 gtgctccaca tcgggtgtac ggcgctgcca atggatggga tcagcggcgc aaagcgggaa
     4981 aaaaatctca aggatggaac tcactcactt tctcctgtct gatgccagag aaccgaaaag
     5041 tgttgtgggc ccatgcggac aattaacgag ttcatcgtca gttcaatctc attcacggtg
     5101 atatctgaac gcagccagaa gtaattgctg tccacgctca ggacggtttt tagctgtttt
     5161 ttagtacgct catcgacgtc agcaagtagc ggctgtgcaa gaaactgatc gacatcttcc
     5221 cagcccttcg ccggacgttg ttgtaataac gcccgcgcct gaacggggct taaccacggg
     5281 tcaaacagcg cctcaagaat cacactttgc gtgacgtcta aagtattgat gttgatttgc
     5341 tggcggatca tcggcagcgc acagaccagc ggtttcagtt tttgataaag cccggcgtcc
     5401 attccctgta ccacgcgcat ctcactgata tcagccagcg gttgattggc ggcgtagaac
     5461 ggcaccgaac gggcgagata ctcgctgtct tcacggccca gacgtgtctg cacgctgcgg
     5521 tcctcgtcaa taaactccca caggctttcg gctatcagtt cggcccgata agcaggcaca
     5581 tccaggcgcg agatcagggc aatcaactgt tgtaccgcaa gcggacgcga cgccgttgtc
     5641 ggctgagcaa gggcattcag gttaaagcaa gcctgtgcgt cacgcagggt gaccgcaatt
     5701 tgcccctgcg gcagagggaa gaaacgaggg ccggatgccc acggctgtgc cagatgcacg
     5761 cgcttttcat ttttcaggct cagactgagc gcgctcagcg ccaggctttc cgcactggcg
     5821 ctgtaccaca gcgcctgctg gtactcctgc tggctgcgcg ttcgcccaag ttgttgctgc
     5881 attcgcccgg aaagcgtgat ggttaccagc atcataaccg ccagcaatac cagcaccacg
     5941 accagtgcca ttccgcgttt tggtggtgag gtgatcatga ttattgcggc ccgcgtaaca
     6001 accagatgcg ttcaatctcg ccccattgcg gcgaatgcag ggtcatgcgt accgccacag
     6061 ggatcgcctg tactgacgac cagctctcct gccagcgcgt gccgtcgtag aactgcaaac
     6121 ggagcgaatc ggccggaatc aacttttgca ttgtcggctt cacgctgcct gcggcatcgg
     6181 tcagcggcca ggctaaccgt tcgagataac cgtcatgaat gcggtaaccg acggtgagca
     6241 gattgctgcg cggcagacgc atcaacgggt taaccacacc gccgcgcaca aaacgcattc
     6301 cttcactctc agacgccagc acgccagcgc cagccagtaa agctggttca cgctggccct
     6361 gatcgcctct taccggacgc ggcatcattt gggtcagatc gtgggtcaga aaactcatcg
     6421 tttgctgcat gaggtttagt ttttgatcgt gatcggcgac ggcgctattc acgcgcgtga
     6481 cgccgttagt cacctgctgc gccatcagtg ccagcgaggc aaaaatggca attgccacca
     6541 acatttccag taacgtgaaa ccagcgcgag tccttctcac tgttggcctc ccacggcgct
     6601 aaaccaggcg cgtcgtgact gaatcaccga cgaaaagtct tcgtgacggc tgacttcaat
     6661 atccaccgcc tggagcagcg cgttgcctgt attcaatggg attgttcgcc agtaccagcg
     6721 gcttcctgcc atatcgctct cgccctgatg cctgaccgcc cgctgctcac gggtcatcag
     6781 gtagagcagc gcagtctggt tttcagctac ccagcccgct accgtttttt cctcaagaac
     6841 gtgctgatta ctcagcgcac cgctggcaat ctgtaacacc gccgtggcag ccagcgcaaa
     6901 aatcgccagc acgagcatca cttccagcaa ggtaaatccg cgcttcatgg catcctctca
     6961 tcacattgat tgagggataa cgcgccatcg tgtgccagct ttaccgctca acatgcgttt
     7021 tgcgccgccg agtagaagcg cagcgtaaac ggcgtggcgg gttcaaaagg cgaaaaacgg
     7081 atctgcggcg tcttcttttt cgcctctttt tgcaactcca gttcaatatc gtgcagcgtc
     7141 aggcgacgac gttgcagctc cagcgcatat tccttctgcc agatatcact tccgggttgt
     7201 aacagcatct gcacctgttt cggcaccgta acctgtgccg ataaccgggt cgcagaaacg
     7261 ggcaaccact gcccctgacg acgctgcata aactgatagc caggcgggtc gattagcaca
     7321 ccgagtgtct gtccttcaat cactgccctg tccttaaact gcgcaaagcg agtcagaaaa
     7381 tcctgtgccg cttttttcgc gggcggttca gaatcggtcg caaacgtctg taccacgccc
     7441 gcactggcaa ggccgataag gaaaatcacc agcatgattt ccagaagtgt gaatccgcgt
     7501 tcaggcactg cttactgaaa ctcctgcaaa ttccagttac cgatatctgc gccagcgcct
     7561 tccccgtttt cctgaccatc cgcccccagg gtataaacat caaacagtcc tttttcaccc
     7621 gggctgagat actgataatc attgccccac ggatcctttg gcagtcgttt gatgtatcca
     7681 ccagtacggt agttgcggga gtccgccata ttggctggct gctggatcag cgcttccagc
     7741 ccctgttctg tggtcggata acgcccgtta tccagtcggt acatatctag tgcgttttcc
     7801 agcgccacga tatcgctgat ggctttttgc cgattggctt tctctttgtt gcccaacagg
     7861 ttaggcacca ccgtccttgc caggacgcca agaataacaa tcaccaccat cacttccagc
     7921 agggtaaaac ctgcccgtgg tttgtgtgtg cgggataacg aattcatgtg ataactccgt
     7981 aaattacatt ccaaccatat tattcagttg cagcatcggc tcgaggatgg cgatgacaat
     8041 aaacagcacc acgcccgcca tcatcaccac cagcgcaggc tcaaacagcc ccaacgccag
     8101 ccccacctgg gtatcaaatt cccgttcctg gttaacagcg gcctgctcaa gcatggtttc
     8161 cagttcgcct ctctgttcgc cggaagcgat aatgtacagc atcatcggcg ggaacaggcg
     8221 caactccgcc agcgcggcgc gcagactgct tccttcgcgg acgcgatctg ccgccagcag
     8281 cagttgttgt tcgacatagc gatttgccga cacggcggca gcggtctgaa tgccttccag
     8341 tagcggaaca ctgctggcgg tgaggatgct taatgtgcgg gaaaaacgcg ccgtattcag
     8401 cccgcgcgca acacggcccg tcacgggcag acgcagcaag gttttatccc agcgcaggcg
     8461 catagtagga tttttaagta gccattgccc cagcaccaga agcgccagca atcccgccag
     8521 ccagtaaacg ccgctggcct gtaacgcatc actcatagcg ataagcgcac gggtggagga
     8581 gggtagcgcg tgtccgagat gatcaaactg ttcgatgatt ttcggcacca ctgccgtcag
     8641 caaaatagtg accacgcccg ttgccaccac cagcataacc agcggataga gcatggcctg
     8701 caatagacgt gatttcaggc gctgtcgctg ttcggtgtaa tctgccaggc gattgagcac
     8761 cacgtcgaga tgcccggatt tttctccggc agcgaccatc gaacaaaaca gggaatcaaa
     8821 gacgcgggga tgttcgcgca ggctgtccga cagggtataa ccttcctgaa tccggctgcg
     8881 cagcgccatt ccgaggctct tcacatgcag tttttcactt tgctcactga ccgcctgcaa
     8941 gcaggtttcc aacggcattg ctgcctgcac cagcgttgcc agttggcgcg tgaacagcgc
     9001 cagatctgcc gccgccacgc gacgatgtgc gtgccgccga cgctgcaaca ttccccctgc
     9061 cgatgcattc atcctggctt caatgtgcac ggggataagc tctttaccgc gcaacaactg
     9121 gcgggcatga cgcgcggaat ccgcctcaat catgcctttg gttttgcgac cattacgctc
     9181 cagcgcctga tagtaaaaca gtgccattac gcctccatgg ttacccgcag aacttcatcg
     9241 agagaggttt ctccggcgag cactttctca atgccgttgc tgcggatacc cgcagagtgt
     9301 tgccggacat aacgttccag ctccagctcc ccggcctgac ggtggatcaa atcacgcaat
     9361 gtggcatcca ccacgtcagc tcatggatgg cagtccgtcc gcggaaacct ttgtgattac
     9421 aggcggggca gccctgcgga tggtacagag tgacggttcg cgcatcggta atgcccagca
     9481 ggcgtttttc ttcgtcagtg gcaggcgcgg cctgacggca gtcgggacac agcgtgcgga
     9541 ccagtcgctg cgccatcacg cccgtcagac tggaagagag caggaaaggc tccacgccca
     9601 tatcctgcaa acgcgtgatc gcccccaccg ccgtgttggt atgcagcgtg gaaaggacca
     9661 ggtgtccggt aagcgatgcc tgaacagcga tttctgcggt ttcggtatcg cggatttcac
     9721 cgaccatcac cacatccggg tcctgacgca aaatcgcgcg caggccacgg gcgaaggtca
     9781 tgccgacgcg ggtgttaacc tgtgtctgac cgatcccttc aatcatgtat tcgatggggt
     9841 cttcaaccgt gagaatgtta cgcgaatggt tgttcagctc ctgcaatccg gcgtacagcg
     9901 tggtgctttt gccggaaccc gtcggccccg tcaccagaaa gatgccgtgc ggtttgtgta
     9961 acagctggcg caactgcacg gtcagttgct ggctaagccc cagacgctcc agcgtcaggc
    10021 gggcctggtt tttgtccagc agtcgcagca ccacgcgctc gccccaggca gaaggcatgg
    10081 tggagacacg cacgtcaatc gcccggccgc ccagcagcag cgcaatacgt ccatcctgcg
    10141 gtacgcgctt ttcggcgata tccagccgtg ccatcacctt gatacgcgat accagcagcg
    10201 aggccagttt gcgccccggg cgcagcattt catgtaatgt gccgtcaacg cgaaaacgga
    10261 tcaccagact cttttcaaac gtctcgatgt ggatatccga agcgccttct ttaatcgcct
    10321 ctgccagcat ggcgttgatc agtttgatga tcggcgcatc gtcgtcactt tccagcagat
    10381 cttccgtttc tggcagttct tccgcgagag taaaaaagtc ctcagcagaa ccgagatctt
    10441 ccatcagctg gcgagcctcg gaagagtccc gctgccagac cgcattcagc cgctgttcaa
    10501 attcggcctc gtcgatttgc cgcagcgtaa agggcgcgtt cagcccccgc tgcagctcct
    10561 gcaaaacaga gagcgatagc ggatgaacgt ggaggatctc cagcgacgct tcgcaccatg
    10621 ccaccaggct aaaccgacgg ctgaaactgt agggcagacg cacggtcttg gcggtggttt
    10681 cctgtgctac aggcaccatt aacgcgttct cccggcattg aggaacgcgc gaacttccgg
    10741 cggtaaggcc tggttttgcg ctggcagtac cggctgtgcg gtgtgcggca tcaggcttaa
    10801 accttgctca tcacgataga tctgttcggc gcgcatatag ttatatttgc gctgcgacac
    10861 gccgtctgcc gccataccgt catgcagaat ggtcgggcgg ataaacacca tcaggttacg
    10921 tttttctttt ttatccgccg tcgatttaaa caggttacca atcaatggga tatcgcccag
    10981 cagaggcact ttcgccacgc tttctcccgc ctgatcgtcc atcagaccac caagcacaat
    11041 cagctcacca tcgttagcca gcacggtggt tttcagtttg cgctcaccaa acaccacgtc
    11101 gaggctggtc tgtccttcca ccttcgagac ttcctgctca atcaccatct gtaccgcgtt
    11161 accttcgtta atctgcggcg tgactttcag catgatgccg acttttttcc tttctactgt
    11221 attgaaagga ttgctgttat tggagccaac ggtagagccg gttaataccg gaacgtccta
    11281 gcccaccatg aagaaggctt cctggttgtc cagcgtagtg atgctcggcg tggagagaac
    11341 gttcgagctg gagtcgtttt taaccgcctg caccagtgcc atccagtcgc ctttcaccac
    11401 gccaaccgcc gtaccgctaa atccagaaag aagctgggcg agtgtggaga gatcgccgtt
    11461 ggtatccgga ttaatggtgg tagcgccgtt ttcactgatc accgtcgagc ctttctgcgg
    11521 tttcgcctga gaaatggctg cccccagcgt accgataggg atctgcgtac cattagcaaa
    11581 ctgcattaat ccggcatctt tcgatgccca ctgcacgccg aagttgatat tgctaccttc
    11641 ggcaacttcc acgatcaacg cctcgacgtg cacctgagca cggcgaatat ccagttgttc
    11701 aattacgctt tgcagcgact gcatgatgtc ctgcggcgcg gttacaatca gggcattact
    11761 gtgtttgctg gcggcgatgg atacaacctc acgcccgcta ccgaccgtgc cttccgcctc
    11821 ttctttagcc gccgtgagcg tgccgctgac ctgtttcagc acatcgacca gatcttcggc
    11881 tttgctgtat ttgagataga aaacctggct gttgccgctg cgttccattt ctgagtccag
    11941 ccgacggatc aggcggcgca ttttgtcccg cgtggccggg tcaccactga caatcacact
    12001 gttggtgcgt tcgtcggcga caatttgaga tttcagcgtc gcaggctggt tctcgccgct
    12061 gtttttcgtc aggctttcca gcacgcgggc gatttccgaa gccgaggcgt tatccagcgg
    12121 gatcacctct tcggtgcgat tacccgcgtg atccacgcgc tggatcactt ccgtcaggcg
    12181 ctccacaacg gaggcgcgtc cggtgagcat aatcacgttg gagggatcgt aattaacaac
    12241 gttgcctgag cctgcgctgt cgatcatctg gcgcagaatc ggtgccagct cgcgtaccga
    12301 cacattacgt accggcacta ctttggtgac catttcatcg cccgcgtagt tgtcgctgcc
    12361 ttcaccgacc agcggcagcg gctcgacttt cgcagcgctg gatttcacca ccttcagcac
    12421 gtcgttttcc atcggtacga cggcataccc ctgtgcttcc agcaggttaa ggaatagctg
    12481 gtaatactgg cgttcattga gcggggtcat ggtgcgaata ctcactttcc cctgtacgcc
    12541 cggccccata atgatggttt tattaaggtt agcgccgacg gtttcgatga acgatttcag
    12601 gtcggtatct ttaaaattag cggtgaaagt ggcttcttcc gcccagaccg gtgaactgca
    12661 taatgccgct gccagcacca gcggcagtaa acgctttttt gttttgaggc cagttgtctt
    12721 cttacgccag accgacaagg taatatcacg ccaaaacacg atgaatgatt ctcctggatt
    12781 aaatgcggtt agcgcagcgc gatggaaatg tcgtggcgcg cacccttgcg taaaaccgta
    12841 agttgaatgg aatccattga aggtaactgc cgcatcagag caatcattgc tcgtggatca
    12901 gtgaaatcct gctgatttag cgcaatggcg atatcgcctt ccttgaaacc gctggcatcg
    12961 aacagagaac gatctgcccc cggcttcact gcataaccga caattccctc cttacgcaca
    13021 ggcgtaagct ggatatagtt aaaaattttc tgcggatctt tcgccagtgc ctgacgcacg
    13081 gcagtcggta tctcaactgg cgcactgaca gcaggttcag caacagcttg ctttgcttcg
    13141 tcactgacag cttttttgtt ggtcgcggca acggtggaac gctcctcttc agccaggctc
    13201 aggcgctcta tttttccctg atagcgcagc atcacatggt cgcgattgat ttcctcaatc
    13261 accgcgttgt gcgagccaag cgtttcaccc tgcaaataga cctgctgttt accgccttct
    13321 tcaataaccg cgccgggtct ggcaccaaag gcgatcccgc gcgcaccaca
//
LOCUS       DAJTOR010000106        13369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTOR010000106 DAJTOR010000000
VERSION     DAJTOR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.106"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            6..200
                     /locus_tag="OO249_002749"
     CDS             6..200
                     /locus_tag="OO249_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5636986.1"
                     /translation="MPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            215..589
                     /locus_tag="OO249_002750"
     CDS             215..589
                     /locus_tag="OO249_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HCR5636987.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            685..894
                     /locus_tag="OO249_002751"
     CDS             685..894
                     /locus_tag="OO249_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HCR5636988.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDVMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            942..1202
                     /locus_tag="OO249_002752"
     CDS             942..1202
                     /locus_tag="OO249_002752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5636989.1"
                     /translation="MATLRELIIKIVSFRGTHIDAFFRRASSAAHTNARGLITVDLFC
                     CTEAIARQVPEAGSEVLSSVIASGREVLTRGLIAGDDVLTRE"
     gene            complement(1337..2938)
                     /locus_tag="OO249_002753"
     CDS             complement(1337..2938)
                     /locus_tag="OO249_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HCR5636990.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDADIAALTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKMIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDV
                     LMRLPEWPEERLAELLPLEGFTFSG"
     gene            complement(2945..3337)
                     /gene="tnpB"
                     /locus_tag="OO249_002754"
     CDS             complement(2945..3337)
                     /gene="tnpB"
                     /locus_tag="OO249_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5636991.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKVRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTQNTLLTQ"
     gene            complement(3324..3653)
                     /locus_tag="OO249_002755"
     CDS             complement(3324..3653)
                     /locus_tag="OO249_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5636992.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            3698..6958
                     /locus_tag="OO249_002756"
     CDS             3698..6958
                     /locus_tag="OO249_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5636993.1"
                     /translation="MTEDPCEDDFPVTTLKISANSQSFQSEIQRASRMGSEYYRTLQN
                     GGRHAAAAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATEHLISLADEWGSVNA
                     RLKQASQSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVT
                     EAISTGLKLSGAGVAESGSVITQFSQALAQGVLRGEEFNAVNENGDRVIRALAAGMGV
                     ARKDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSGSITKVENAFMAWVGE
                     VNEACGVTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTA
                     ARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNR
                     NIAARSAAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQAWESA
                     RQYALTIDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQ
                     YVLANPGWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLALMQEKAQSIQDVLA
                     GLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRL
                     PQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYIS
                     NALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTA
                     QGELKTLTEMQKQELLRNAAQIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQ
                     GERLRERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQE
                     AFYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNK
                     VEWRSWASSVLQEISKVLMNAAIVNGIKTAANSMSGAGGFIGSIGSWLGGAVANAKGG
                     VYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMN
                     GSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            6951..7292
                     /locus_tag="OO249_002757"
     CDS             6951..7292
                     /locus_tag="OO249_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5636994.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            7292..7990
                     /locus_tag="OO249_002758"
     CDS             7292..7990
                     /locus_tag="OO249_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5636995.1"
                     /translation="MQDIHEESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            8001..8744
                     /locus_tag="OO249_002759"
     CDS             8001..8744
                     /locus_tag="OO249_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5636996.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAPPGDILLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACM"
     gene            8642..9322
                     /locus_tag="OO249_002760"
     CDS             8642..9322
                     /locus_tag="OO249_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5636997.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGSFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            9563..13036
                     /locus_tag="OO249_002761"
     CDS             9563..13036
                     /locus_tag="OO249_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5636998.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPESFESSGSETVLGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKCLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTCRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTLGYFQITAVPRLAVYDPTV
                     QFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTVTVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSCVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGISIS"
     gene            13104..>13369
                     /locus_tag="OO249_002762"
     CDS             13104..>13369
                     /locus_tag="OO249_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5636999.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWH"
BASE COUNT         3037 a         3327 c         4182 g         2823 t
ORIGIN      
        1 ggggaatgcc gccgtataaa tacgcctgga agaaggatgg tcagccggtt gacgggcaga
       61 cgacagacac cttcagtaag ccaggtgcgc agtccgctga tgcggggaaa tatacctgcg
      121 tggtgaccga ttcggcagag aaagcacaga gtgtgacgtc tgttgaatgc accgtgacag
      181 tgagcgcagc tgccggataa ggggatgggt catcatgaaa aaggatctga aaacgctggc
      241 gctggccaga ctgtcagggt ttcgtcataa aacggtgaag gtgccggaat gggggaatgt
      301 cagcgtggtg ctgcgggagc cttcggcaga ggcctggtat ctgtggcagg aagtgctcaa
      361 tggtgatgga gaggatgacg ataccctgtc ggtggtggcg aaaacccgcc gtaacttgga
      421 agcggatgtg acgctgttct gcgatgtcct gtgtgatacg gacctgcagc gggtgttcac
      481 tccggacgac cgtgagcagg tgctggccgt ctatggtccg gtacatgccc ggttgctgcg
      541 tcaggcactg gaactgatcg ctgatgcaga gtcggccaga aaaaagtagc ccgcccggaa
      601 attcgctttc tgatgcgact tgcgctccgt ctggggcgca ccttatccga actgcgccac
      661 agcctgagtg cgagcgaggc gatgatgtgg atggagttcg acaggatatc cccgctgggt
      721 gatgagcgcg gggatatccg taatgcacag atcgtgaaag cggttttcgg ggcacagggg
      781 atgaatgttg cactgaagga cgtcatgctc tgctggggcg aggatgagga taagccggag
      841 gtggatccgt ttgcggcgct ggaagacgcg ctgagctttg cagcacagtc atgaatgatg
      901 agaaccgctg aggcggtttt tttacgcccg gagaaaggtg aatggcgacg ttacgtgaac
      961 tgattatcaa aattgtaagc ttccggggaa ctcatattga cgcgtttttc aggcgcgcgt
     1021 catcggcggc gcatacgaac gctcgtggtt tgataacggt tgatttattt tgttgcacag
     1081 aggcgatcgc caggcaggta ccggaggcgg gctctgaagt actgagtagc gtgatcgcgt
     1141 caggccgcga agtactgact cggggattaa ttgcaggtga tgatgtactg acccgggaat
     1201 gactccgggc tgattcaggt tcttattcgc tgtcagcccg cgtggttgct ggtctggctc
     1261 atttcctgca gtcaggagtt caactcccga ctgcaggtta tggcccggtg cacgaacacc
     1321 tgacggcagg tatcactcac ccggagaagg taaatccctc aagaggcagc aactctgcca
     1381 gtcgctcctc cggccactcc ggcagacgca tcaggacgtc tgtcaaccag gcatgcggct
     1441 ccagaccgtt gcgtttcgcg gtttccagca agctcattat ttgcgcggcg cgttctcccg
     1501 ccatctgcga accggcgaac agccacgatt ttctgcccag aaccacgttt ttgatggccc
     1561 gttcacacac attattatcc agcggcaccg caccatcttc caggaagcgg ctcagttcca
     1621 cgcgatgaga cagcgcatag gcaatagctt tgtgtaatgc ctttcccgga gaacattgcg
     1681 gttcctgctc ttcaagccat gaccacagtt cttccagtat cggacgggca taacgctgtc
     1741 gccactggcg gattttttcc acggggcggc tactgatctt cttctcaaga cggtacaacc
     1801 ccgcgatttt cttcacggct atggcagccc gtggatcttt actgatttta tacaggtcgg
     1861 cgaagcccct gcgggcatgg gcccagcatc cggccagcgt gatctccggc actttgtttg
     1921 ccagagtccg gtaagcttta tgtccgtcga caaccagcgt cccgccccag ccctgaagcc
     1981 agttttcagg atactcatgg ctacgtccgg tccggcagtc gaagcacaca actgacggtc
     2041 ccgtcctttc tccactgacg tatgcccaca gataaccgga gcaggattta ccgcctttct
     2101 tcgtgttcag gatcttcagg gtagtctcat ctgcatgcac caccggacgg tttatcaact
     2161 cgcgatggag taacgccgcc aggggagata atgcggcacc cgccgcgcca accatgtcag
     2221 ccatcgaact gacgggcagc ccgacatctg aacgggcaaa gacctgttgc tggcgataca
     2281 gaggcaggtg gtcaccgtat ttattgatga tcacctgtgc gatgactgat ggctcaacgg
     2341 cacttttcgg gaggatatgt gccggcattt caccgctgaa caccttctgg caacagggac
     2401 aactgtattg cggacggaca taacggttca ccacaaagtg agcgggaata tactccagct
     2461 tttcactcac cgcatcgcgg atatagtgca gcggctcgtc acattcaggg caatggtcag
     2521 tgtccggctg gatcattttt tccacccgtg gaagatgaac cggtaagggt ttgcgtatcg
     2581 ggcgtgaacg ggacgctttt tcatcttctt caggggattg cgggagcagt ttatccagat
     2641 gtgcggtaag cgcggcgata tcggcatcaa catcctcttc gaatagggag cgttgcatac
     2701 cagccagcgt ttcacatttt tttccgaagc gctgttgacg aaccagtttc agcatttctt
     2761 ccagaagctg gatgcgccgg cttttctcca gtaattcccg ctctttttcg gcattctccg
     2821 acatgacttc ttgtaccatg gcaagtgcca tagcacgcag tttttcgatg tcattcgtgg
     2881 tgttgagagc ggagatatcc atacagggga gtataccact ccagatacgg atattcatta
     2941 tttttcactg agttagcagt gtattttgcg tgttttcagg gcgcagttca ggttcattct
     3001 gccagaccca tttcgtcagg tcatgtccct taacctgctg ccagtcaaca ccggcaatca
     3061 gccagttaaa ctcgtcggga gtgaggtgcc aggcagcgtc atttgcacgt ggccagcgga
     3121 agctgccttt gtgcagacgg cgggtacaca accacacccc gtgtttatcc caccggagaa
     3181 ctttgatacg cgaacggact ttgttaacaa agacgaaggc ggctccctca tgccatgtcg
     3241 accggagttc atcctgtata tactgcgtca gtgagtcgat gccccggcgc atgtcaacag
     3301 gtttaattgc gatgaagaca ttatcaggac tgagcatatt tgagtgccct gaagacatcg
     3361 gtaagttgtg acggctgaca gcacatccgt actccaccgg gaaggaagag cgtgactggc
     3421 tcattcgtga cgggctgtgg tacatcagca tgcagtggtg gcgcaatatg aacgggaaga
     3481 acggtcggtt catttgcccg gcgttcagct tttgcgacat cctgcggcca ttcgcgaagt
     3541 gatgtgaagg gaatatcgtt gagttcacag tattgctgac gtgtaagacc actggcgcgc
     3601 caggcagcaa cgtgctgcct tttctgttca atggtccatc ggggttttga catcgattat
     3661 ctccggtaag ttggtgaatg gatgtcggta atactaactg accgaagatc cctgtgaaga
     3721 tgacttcccc gtaacgacac tcaaaatttc ggcaaattca cagtcattcc agtcggagat
     3781 ccagcgggcg tcccgtatgg gcagtgaata ttaccggacc ctgcagaatg gcggacgtca
     3841 tgccgctgct gccgcacggg aacagcgccg cgcccttgca gaactgaaca gtcagttgac
     3901 ggaaattcgc ggttctgctg tcggaatggc tggcgcattt gccggtgcct ttgccaccga
     3961 acacctgatt tcgctggccg atgagtgggg ttccgtgaat gcccgtctga aacaggcatc
     4021 tcagtcatcg gatgaattct cgtcatcaca gaaagtgctg atggatatca gtcagcgaac
     4081 aggtaccgcg ttttcggata atgcggccct gtttgcccgt tcggctgcct cgatgcgtga
     4141 atatggttac agtgctgatg atgtgctgaa ggtgacggag gccatttcga caggactgaa
     4201 actgtcaggg gctggagttg cggaatccgg ttcggtgatc acccagttca gccaggcact
     4261 ggcacagggt gtactgcgtg gcgaagaatt taatgctgtt aacgaaaatg gcgaccgggt
     4321 gatccgcgcg cttgctgcgg ggatgggtgt ggcccgtaaa gatctgaagg caatggcgga
     4381 tgacggaaaa ctgacagcgg ataaagtggt ccctgcgtta atcagccagc tggggatatt
     4441 acgtgatgaa tatgcggcca tgccggaaac ggtttccggt agtatcacga aggtggaaaa
     4501 cgcctttatg gcctgggtgg gcgaagtgaa tgaggcctgc ggggtgacaa aaacgctctc
     4561 cggcgtgctg aacggtgttg ccggacagat tgataatgtg gcaacagctg tgggggcgct
     4621 ggttgccgtc ggggttgccc ggtactttgg caatatggcc tccggagcga tgtctgccac
     4681 ggcaggactt gtgacggctg cacgtaatga agttgcactg gcggaagcac agttcagggg
     4741 aacgcagatt gccacggcgc gggcaagggc agccgtgtac cgtgctcagc aggccgtggc
     4801 ggcagcccgc gggacggaga tgcagattgc tgcagaggcc cgtctggcgg ccacacagga
     4861 acgcctgaac agaaatattg ctgccagaag cgccgcccag aatgcgctga acagtacaac
     4921 ggcggtgggc tcacgtctga tgagcggtgc gctggggctg gttggtggcg tacccggact
     4981 ggtgatgctg ggggctgcag catggtacac gctgtaccag aatcaggagc aggcctggga
     5041 gtctgcgcgc cagtatgcac tgacgataga tgaaatcgcg cataaaacgc cgtcaatgtc
     5101 tttgcctgaa gcctcagata atgaaggacg aacacgggcg gcgctgacag agcagaaccg
     5161 gctgattgat gaacaggcta gtcgggtgaa atccctgcag gaaaaaatcg caggatatca
     5221 gtatgttctg gcgaacccgg gctggacgac cggtgacgga ttcatgataa accatctgac
     5281 ctcggtgaag accgtaacgg aagggcttgc tcaggcaaca gagcagcttg ccgttgagca
     5341 gtcccgtctg gcactgatgc aggaaaaagc gcagtccatt caggatgtgc ttgccgggct
     5401 ggaagaccgt cgtgtggcgt taattcgtca gcaggcggca gagcagaata aggtgtacca
     5461 gtccatgctg gttatgaacg gtcagcatac ggaattcaac cgtctgctgg ggctgggtaa
     5521 tgaactgctt cagcagcggc agggactggt gaatgtgccg ttacggctgc cacaggccac
     5581 tctggatgat aaacagcaga gtgccctgac aaaaacagag cgtgagctgg ccctgtccag
     5641 actgaaaggg gaagaaaaag agcgtgtccg actggggtat gcggcggatg acctcggttt
     5701 tgtgggtgat ccgtatcagg aggcgagaca acgttatatc agtaatgccc tggaagcctg
     5761 gcgcaataac gaggcgaata aacccaaatc ccggggtgga aaatcagaga cggaaaaagc
     5821 ggaagacagt ttttcccggc tgctgaagca gcagaaagag caactggcac tggcggggca
     5881 gaatacagag ctggcgaagc tgaaatacca gactgcgcag ggcgaactga aaaccctgac
     5941 ggagatgcag aagcaggaac tgctgcgcaa tgcggcccag attgaccagc aaaaaatccg
     6001 ggagcagttg cggtcccggg aagagaccct gaagaatgat aatgtggctg ctcgtgcatc
     6061 aaatgaagcg gaactgctgg ggtacgggca gggagagcgg ctccgtgaac gcatgcggga
     6121 gttgcagcag atccgcgaca gtttccgcca gaaggatgcg gaccttcagt ctcagtatca
     6181 gaccggggat atcagtgagg atttttacag acaggctctg gcacagaacg cgcagtatct
     6241 gagtgaacgt ctgaaagagc aggaagcctt ttatgccgaa tcggatgtgc agcgtgcgga
     6301 ctggcagaaa gggctgcagg agggattcag taactgggtg gataatgcgt ccgattacgc
     6361 ctcacaggca gcacagcttg cgacggaggg tatctcaggg atggtgaata acatcacgga
     6421 gatgctgaac ggaaataaag tggaatggcg cagctgggcc tcatcagtgc tgcaggaaat
     6481 atcaaaagtt cttatgaatg ccgcgattgt caacgggatc aagacggcgg caaacagtat
     6541 gtccggagcg ggaggattta tcggcagtat tggtagctgg ctgggcggtg cggtggccaa
     6601 tgcaaaaggc ggtgtgtata cctcggcaaa cctgagtgcg tacagcaata gtattgtgga
     6661 tacgcccacg tactttgcct ttgcaaaagg ggcgggactg atgggggagg ccggtcctga
     6721 agccattatg cccctgaccc gggcggcgga tggctcgctg ggtgtgcgag cggtgggcag
     6781 tatgaacggc agtgcaggtc tggtgtattc cccggtctac catatcgcca ttcagaatga
     6841 cgggactaat ggccagatag ggccggaagc tgcgggcagc cttgtgcaac tgattgacca
     6901 gcgggtgcag gcggtgatgc tgtccatgcg acgtgacgga ggaatgctga gtggctgaga
     6961 taaaaacgct gcatctggtc ccgcgtgaag ggatgcaggt gagtgagaaa ccgtcggtgg
     7021 cgagggtacg gtttggtgac ggttatgaac agcgccgccc gacgggactt aatgcccgac
     7081 tgaagacgtt tcaggcggtg ttccgggtga cggatgaggc gacccggcga tggctggaag
     7141 agtttttatc gtggcatggt ggttaccgtg cctttttgtg gcgaccgccg aaacataacc
     7201 ggacggtgag ggtggtatgc cgggagtgga gcgtcacaga taacgccagg tacagtgatt
     7261 tcagttgtac gattgagcag gtggtgaact gatgcaggat attcacgaag aaagtctgag
     7321 cgagtcggtt aaatcagagc agtcaccgcg ggtggtactc tgggaaattg acctgacggt
     7381 gcagggtggt gagcggtatt ttttctgtaa tgagctgaat gaaaaagggg agccggtcac
     7441 ctggcagggg cgtaagtatg aggcataccc gattgacggc agcggctttg agatgaacgg
     7501 ccggggcagc agtgccagac cgtcgctgac ggtgtccaat ctgttcggtc tggtcaccgg
     7561 gatggcggaa gacctgcaga gtctggtggg ggccacggtg gtccgccgcc gggtgtatgc
     7621 ccgttttctg gatgcggtga atttcgttgc gggcaatccg gaggcggacc cggagcagga
     7681 gctgagtgac cgctgggtgg tggagcagat gtcgcagctg acagccatga cggcctcgtt
     7741 tgtgctggct acaccgaccg agacggacgg ggcgctgttt cccggtcgta tcatgctggc
     7801 gaacacctgt atgtggacct accgctctga tgagtgtggt tacacgggcg gggctgtggc
     7861 ggatgagttc gataaaccca ccacggatat ccgtaaggac agatgcagca agtgcatgcg
     7921 cgggtgtgaa ctgcgcagga atgtcggcaa ttttggcggt ttcctttcca ttaataaact
     7981 ttcgcagtaa atcccgattt atgacacaga ctgaatcagc gattctggcg catgcccggc
     8041 ggtgtgcgcc tgcggagtcg tgcggcttcg tgataagcac gccggagggg gagcggtata
     8101 tcccttgtgt gaatatttct gcagagccgg aggcgtattt tcgtatcgca ccggaagact
     8161 ggctgcgggc agagatgcag ggggagattg tggcactggt ccacagtcat cccggtgagc
     8221 tgccctggct gagcgaggct gaccggcggc tgcagataaa aagcgcactg ccctggtggc
     8281 tggtctgccg gggggaaatt cataaattcc gctgtgtgcc acatctgaca ggacggcgct
     8341 ttgagcacgg ggtgacggac tgttacacgc tgttccggga tgcataccat ctggcgggaa
     8401 ttgatatgcc ggattttcat cgcgaggatg actggtggcg caacggccag aacctgtacc
     8461 tggacaatat ggcggtcacc ggcttttacc gggtgcccct gtcctctgca ccgccgggcg
     8521 atattctgct gtgctgcttt ggtgcttcgg taccgaacca tgccaccatt tactgcggca
     8581 acggtgagct gcttcaccat ctgcctgaac aactgagtaa acgggagagg tattccgaaa
     8641 aatggcaacg acgaacgcat tctgtctggc gtcaccgcca ctggcacgca tctgccttca
     8701 cggggatttg caacgatttg gccgccgcct cagcctgtat gtgaacacgg cagcggaagc
     8761 cattcgcgcc ctgtcgatgc agatgccggg ctttcgccgt cagatgaacg aaggctggta
     8821 ccagatacgt attgccggtg atgacacggc accggaggcg gtgtatgccc gtcttcacga
     8881 acagctgggt gagggaacgg tcatccacat tgtgccgcga ctggccgggg ccggaaaggg
     8941 tggactgcag attgtgttgg gggcggcagc catcgtgggc tctttcttca ctgccggggc
     9001 atcaatggcg ttatggggtt cagccctggc agccggtagt ttttctgcca ccacgatgct
     9061 gttttcactt ggagccagca tgattctggg cggtgtggcc cagatgctgg ccccgaaggc
     9121 aaaaacaccg gattaccgcg caacggataa cggcagacag aacacgtact tttcctcgct
     9181 ggataacatg attgcccagg ggaacccgat gccggtgcct tacggggaaa tgctggttgg
     9241 ctcccgccgt atatcccagg acatcagcac ccgtgatgaa ggcgggggcg gaaaggtcgt
     9301 ggttatcggg cgacagggat aaaacataaa aaaatcccgc agtgatcgcg gagctgcggg
     9361 gacagacaaa tgaagatcaa tgtgaaggag ttgtttttgt tactcgggca aaaaaacact
     9421 aacgcagcga aattataagc gccacagtca gtgtgtgaaa atgtgaagat attcagaaat
     9481 tttattccgt catgacgcag gcacccggtg aggtgcctgt tgtttttgtg agtgaacaat
     9541 tatcacggta agaggtgatg taatgggcaa aggtggcggc agggcgcaca caccgcgtga
     9601 ggcgaaagac aatctcaaat ccacgcagat gatgagcgtg attgatgcga ttggtgaggg
     9661 accgatagaa ggcccggtga aaggcctgca gagtattctg gtgaacaaaa ccccgctgac
     9721 ggacacggac ggtaatcccg tgatacacgg tgtgaccgcg gtctggcgtg ccggggagca
     9781 ggagcagaca ccaccggaaa gctttgagtc ctccggctct gaaactgtac tgggtgtcga
     9841 agtgaccagg gcaaaaccgg taacacgcac cattacgtca gcgaacattg accgcctgcg
     9901 ggtgaccttc ggggtgcagt cactggtgga gaccacgtca aagggtgacc gtaatccgtc
     9961 ctctgtccgt ctgctgattc agttacagcg taacggtaac tgggtgacag aaaaggatgt
    10021 caccattaac ggcaagacca cctcacagtt ccttgcgtcg gtgattctgg ataatctgcc
    10081 tccccgcccc tttaacatcc ggatggtcag ggagacggcg gacagcacca cggaccagct
    10141 gcagaacaga acgctgtggt cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc
    10201 gaacacggcc attgtgggga tgcaggtgga tgcggagcag tttggtggtc agcagatgac
    10261 ggtgaactac catatccgcg gtcgcatcat ccaggtgccg tcaaactatg acccggaaaa
    10321 acgcacgtac agtggtatct gggacggcag tctgaaaccg gcatacagca acaacccggc
    10381 ctggtgcctg tgggacatgc tgactcaccc gcgctacggc atgggaaaat gtctgggggc
    10441 ggcggatgtg gacaagtggg cgctgtatgc catcgggcag tactgcgacc agacggtccc
    10501 ggatggtttc ggggggaccg agccgcggat gacctttaat gcgtacctgg cacaacagcg
    10561 taaggcgtgg gacgttctca gtgatttctg ctctgcgatg cgctgtatgc cggtatggaa
    10621 cggtcagacg ctgacgttcg ttcaggaccg cccgtcggat gtggtgtggc cgtacaccaa
    10681 cagcgatgtg gtggtggatg ataacggcgt gggattccgc tacagcttca gtgccctgaa
    10741 ggaccggcac acggcggtgg aggtgaatta caccgacccg cagaacggct ggcagacctc
    10801 cacggaactg gtggaagacc cggaagccat actgcgctac ggacgcaacc tgctgaagat
    10861 ggatgcgttt ggctgtacct gccgcggtca ggctcaccgt gcaggtctgt gggtgataaa
    10921 gaccggactg ctggaaacgc agacggtgga cttcacgctc gggtcacagg ggctgcgtca
    10981 cacaccgggt gacattattg aaatctgtga taacgactat gctgggacta tgaccggcgg
    11041 acgtgtcctg tccattgatg ctgccacccg caccctgacg ctggaccgtg aggttaccct
    11101 gccggagaca ggtacatcgg cggtgaacct gattaacggc agtggtaagc cggtgagtgt
    11161 ggacatcacc gcacaccccg cgccggaccg gatacaggtc agtaccctgc ctgatggtgt
    11221 ggagacatac ggggtgtggg gactctccct gccgtcactg cgccgtcgcc tgttccgctg
    11281 tgtctccgtc cgggaaaaca cggatggcac ctttgccatc acggcggtgc agcacgtacc
    11341 ggaaaaagaa gccatcgtgg ataacggtgc ccgctttgag ccgcagtcag gctccctgaa
    11401 cagcgtcatc ccaccggcag tgcagcacct gacggtggag gtgagcgcag ctgacggcca
    11461 gtatctggca caggcgaaat gggacacgcc gcgggtggtg aagggggtgc gcttcagtct
    11521 gcgactgacc agcggaagcg gagaagacag ccgtctggtg accaccgcta tcactgcgga
    11581 tacagagcat cgtttcagtg gtctgccgct cggggaatac accctgacag tcagggcaat
    11641 taacagttat ggccagcagg gcgaaccggc caccaccacc ttccggatta acgcgccagc
    11701 aaaacccgcc accattgaac tgacgctggg gtattttcag ataacagcgg taccgcgtct
    11761 tgcggtgtat gacccgacgg tacagtttga gttctggttt tcggaggcaa aaatcgcaga
    11821 cacatctcag gtggaaacct ctgcccgtta tctggggacc ggcagtcagt ggagtgtatc
    11881 cggcccgcac attaagcccg ggaaggattt ctggttttac gtgcgcagcg tcaacctggt
    11941 ggggaaatct gcttttgtgg aggccagtgg acgggccagc aatgatgcag aagggtatct
    12001 ggggctgttt cgggaaaaaa taggaaaact gcatctggct caggggctgt gggagctgat
    12061 agacaacagc cagcttgcgg atgagatggc ggagatgaag accaccatca ccgaaacccg
    12121 caatgaaatc acacagacgg tcagtaaaac gctggaggac cagagcgcca ccatacagca
    12181 gatacagcgc gtgcagaagg acacaaatga tgaccttgct gcactttaca tgctgaaggt
    12241 acagaaaaca aaaaatggca taccctatgt tgccggtatt ggagcgggga ttgaggatac
    12301 tgatggccag cccctgagca acatactgct gctggctgac cgtattgcga tgattaaccc
    12361 ggaggacggc aacaccacgc cgttatttgt ggcgcagggg aatcagttgt tcatgaacga
    12421 tgtgttcctg aagcggctgt ttgcggtgag tatcacctcg tccggcaatc ccccgacgtt
    12481 ttccctgacg ccggagggca ggctgaccgc aagaaatgct gatatcagcg gtaacgtgaa
    12541 tgcgaactcc gggacgctca acaacgtcac gattaacgag aactgccggg ttctgggaaa
    12601 actgtccgcg aaccagattg aaggcgatct cgttaaaaca gtgggtaagg cttttccgcg
    12661 ggactcccgg gcaccggagc ggtggccatc agggaccgtt accgtcagga tttatgacga
    12721 tcagcctttt gaccggcaaa ttgttattcc ggcggtggca ttcagcggcg ctaagcatga
    12781 gagagagcat actgatattt actcctcatg ccgtctgata gtgcggaaaa acggtgctga
    12841 aatttataac cgtaccgcgc tggataatac gctgatttac agttgcgtta ttgatatgcc
    12901 tgccggtcac ggtcacatga cgctggagtt ttcggtatca gcatggctgg tgaataactg
    12961 gtatcccaca gcaagtatca gcgatttgct ggttgtggtg atgaagaaag ccaccgcagg
    13021 catcagtatc agctgaattt tataacccat atacgggcgc cagaaatggc gcctttttta
    13081 ttgcagaaaa gcgagaggta attatgcgta aagtttgtgc agccattttg tccgcagcca
    13141 tctgcctgtc cgtatccggt gcgcctgcat gggcgtctga acatcagtcc acactgagcg
    13201 cggggtatct tcatgcccgt acgaacgctc ccggcagcga taatctgaac gggattaacg
    13261 tgaaataccg ttatgagttt acggacgcgc tggggctgat tacgtccttc agttatgcca
    13321 atgctgaaga tgagaaaaaa acgcactaca gcgatacccg ctggcatga
//
LOCUS       DAJTOR010000107        13344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTOR010000107 DAJTOR010000000
VERSION     DAJTOR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.107"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..186
                     /locus_tag="OO249_002763"
     CDS             <1..186
                     /locus_tag="OO249_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5637000.1"
                     /translation="CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELL
                     NRRLSESVPCMPHKKAG"
     gene            207..908
                     /gene="mlrA"
                     /locus_tag="OO249_002764"
     CDS             207..908
                     /gene="mlrA"
                     /locus_tag="OO249_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR5637001.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDDGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARRKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            968..1075
                     /locus_tag="OO249_002765"
     CDS             968..1075
                     /locus_tag="OO249_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5637002.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1056..1787)
                     /gene="yehW"
                     /locus_tag="OO249_002766"
     CDS             complement(1056..1787)
                     /gene="yehW"
                     /locus_tag="OO249_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5637003.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFVVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1792..2718)
                     /gene="yehX"
                     /locus_tag="OO249_002767"
     CDS             complement(1792..2718)
                     /gene="yehX"
                     /locus_tag="OO249_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5637004.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVCREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(2711..3868)
                     /gene="yehY"
                     /locus_tag="OO249_002768"
     CDS             complement(2711..3868)
                     /gene="yehY"
                     /locus_tag="OO249_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5637005.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3875..4792)
                     /gene="osmF"
                     /locus_tag="OO249_002769"
     CDS             complement(3875..4792)
                     /gene="osmF"
                     /locus_tag="OO249_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5637006.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQISGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5044..7341)
                     /gene="bglX"
                     /locus_tag="OO249_002770"
     CDS             complement(5044..7341)
                     /gene="bglX"
                     /locus_tag="OO249_002770"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5637007.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPFTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGSKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDMVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7537..9252
                     /gene="dld"
                     /locus_tag="OO249_002771"
     CDS             7537..9252
                     /gene="dld"
                     /locus_tag="OO249_002771"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5637008.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPSSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALQRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9291..10223)
                     /gene="pbpG"
                     /locus_tag="OO249_002772"
     CDS             complement(9291..10223)
                     /gene="pbpG"
                     /locus_tag="OO249_002772"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5637009.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10397..10984)
                     /locus_tag="OO249_002773"
     CDS             complement(10397..10984)
                     /locus_tag="OO249_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5637010.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11154..11732
                     /locus_tag="OO249_002774"
     CDS             11154..11732
                     /locus_tag="OO249_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5637011.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(11862..12623)
                     /locus_tag="OO249_002775"
     CDS             complement(11862..12623)
                     /locus_tag="OO249_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5637012.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(12676..>13344)
                     /locus_tag="OO249_002776"
     CDS             complement(12676..>13344)
                     /locus_tag="OO249_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCR5637013.1"
                     /translation="IEARLSALTNNQTKSLKLKPVALPKVASQLPDELGYSLLARRAD
                     LQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTL
                     PIFDSGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIE
                     RDTLRVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGG
                     GYKR"
BASE COUNT         3208 a         3734 c         3372 g         3030 t
ORIGIN      
        1 tgctggtcac ttgccgcagg agctaatcct agttttatcg caagccagat gggccacaca
       61 aacgcacaaa tggtattcaa tgtttacgga gcatggatga aagacaacaa tcacgaacag
      121 atagaacttc ttaacagaag actatctgaa agtgtcccat gcatgcccca taagaaagcg
      181 gggtaaaata aaaacttgca aaatcaattg gtttaccttt aatccctgtc acgttacgcg
      241 cgtggcagag gcgttacggg ttgctgaaac cgcaacggac agacgacggt catcgactgt
      301 tcaacgatgc cgatattgac cggatccgcg agatcaaacg ctggatcgac aacggcgtgc
      361 aggtcagcaa agttaaaatg ctgctcagta atgaaaatgt tgatgtgcag aacggctggc
      421 gcgatcagca agaaacatta ctgacctacc tgcaaagcgg caatctgcat agcctgcgaa
      481 cgtggatcaa agagcgcggt caggattacc ccgcccagac actcaccaca catctgttta
      541 ttcctctgcg ccgacggctt cagtgccaac aaccgactct ccaggcgctg ctggcgatcc
      601 tcgacggcgt actgatcaac tacatcgcca tttgtctggc ttcggcacgt agaaaacagg
      661 gtaaagatgc gctggtggtt ggctggaata ttcaggatac cacccgtctg tggctggagg
      721 gctggattgc cagtcaacaa ggatggcgca ttgatgtcct cgcccactcg ctcaatcaac
      781 tacgccctga actattcgaa ggccgtacat tgctggtgtg gtgcggtgaa aatcgaacct
      841 ccgcccaaca gcagcaactc accagttggc aagaacaagg ccatgatatt ttcccactcg
      901 gcatttaatg attcgttaac aaatgcgctt tactgtacaa tcctttcgtt aacataagga
      961 gtgcaatatg cgcatagcta aaattggggt catcgccctg ttcctgttta tggcgttagg
     1021 cggaattggt ggcgtcatgc tcgcaggtta tacctttatt ttgcgtgctg gctaagcgcc
     1081 tgcaccagcc tttcaaacag gcggtctgca atgatcgccg ccagtgccac cagtaacgcc
     1141 ccctggatca catacgcggt attaaatccg ctaagcccga tgatgatggg cgtacccagc
     1201 gtgctggccc ctaccgttga ggcgatcgtc gccgtaccaa tgttgataat caccgaagtt
     1261 cgcacgcccg ccagaatcac cggagccgcc agcggtagct cgaccttacg cagtcgctga
     1321 ccacgactca ttcccatacc tttcgcaact tctgtcacgc tggcatcaat cgctcccagc
     1381 ccggcaagtg tcgcctgcag gacgggcagc acaccgtaaa ggatcaaggc gataatcgct
     1441 ggttgcagac caaagccgat caccggaacg gcaatcgcca gcactgcgac aggcggaaaa
     1501 gtctgtccaa cggcggcaat agtttccacc agtgggcgaa attccgcgcc ccacgggcga
     1561 gtgacagcaa ttccggcacc agtgccaatg atcaccacaa acaaactcga aattcccacc
     1621 agccagaaat gagccagtgc cagagctgca aaactttctt gctgataaac gggtcgtggc
     1681 agttgtggga acaaggcagc aaacagcggc tggctgtaag gcagccagaa aatcagtgcc
     1741 acaaacaaag caatgagcca gaacagcgga tcgcgcaaca tcttcatacg cttacgcctc
     1801 ctccagcaga tcctgaaaat gcagcgtgcc gcaaggctgg ccctgcgtgt tcaccaccgg
     1861 cagcacctcg catccccgcg cgacaaacag ggagagcgca tcgcgtagcg tcatctcttc
     1921 tgccagtgcc tcaccttctg cccgttcttc gcgacacacg taatccgcca cactacgtaa
     1981 cgaaagcagg cgcacaccca gttcgctgcg cccaaaaaac tggcggacaa aatcgtttgc
     2041 cggacgagtc agcatcgtca gcggattgcc ctgctgcacc acttcaccgt gatccatcaa
     2101 taccagatgt tctgccagcc gtagcgcctc atcaatatca tgagtgacca gcacaatggt
     2161 acgccccagc aaacggtgaa tgcgcgtcat ctcttgttgc aacgcgccgc gcgttaccgg
     2221 gtccagtgcg ccaaaaggtt catccatcag taagacttgc ggatcggcag ccagtgcgcg
     2281 cgccactccc acacgttgct gctgaccacc ggaaagctga tgcggataac gctcacgtaa
     2341 atttgactcc agccccagta gcgccattaa ttcgtcgata cgatcgtcaa tccgcgcccg
     2401 cgaccatttt tgtaattgcg gcacggtagc gatgttttgc gccacgctcc agtgggggaa
     2461 caggccaata gattgaatag catagcccat ccggcggcgc aactccagta ctggcagcga
     2521 gcgaatttct tctccggcaa agcggatcac tccgctgtca tgctccacca ggcggttaat
     2581 cattttcagg gtggtggatt tgccggagcc agatgtgcca atcagcaccg aaaaactccc
     2641 ttcctgaaaa ttgagattga gatcgttaac ggctttttgt gcgccgaaca gtttgctgac
     2701 atggctaaat tcaatcatta cgtttcacct tcagcagtgc gataagcaaa tcgaacagcg
     2761 cgtcggtaag caccgccaga acaattaccg ggatcacccc cagcaacact aaatcaatgg
     2821 cgctgcttag cagcccctgg aaaaccagcg caccaaaacc gcctgcgccg attaacgccg
     2881 caatcaccgc catacctaca gtttgcacca tcaccacccg caggctgcgc agaaataccg
     2941 gtaacgccag tggtaactga acatgcagga atcgctgcgc cccgctcatg cccatcgctc
     3001 tggcactctc cagcacatcg cgcgggatct ggttcaaacc gactaccacg ccgcgcacca
     3061 gcggcagcaa ggcatagagc accagcgcaa tcagtgcggg tgtcattccg gttcctgcta
     3121 tgccgagctt ccccagccac ggaaaggccg tcaccagcgc ggcaagcggc gcaatcaaca
     3181 ggccaaagag cgccaccgaa ggcacggtct gaatgacatt gagcagagaa aaaattgccc
     3241 cctgccgagc agtggaaaag tagcaccaga tgcccaacgg cacaccaatc actaacgcag
     3301 gcagcaccgc accaaacagc aacgtcagat gttgtgccag cgcgtcgtca aacacatcct
     3361 gacggttggc gtattctttc attagtgaga gatcgttaag cgtgccggag tacagcaacc
     3421 acagcggaat aatggcaatc tgcatatgca acaaccagcg ccacagcgga tgcgtggaga
     3481 ttcggcggat ggcatcgcta caggccagca atgccagcgc cgcagccagc cagaaaccac
     3541 tgccgaggct ggtacgcgcc agcgcactgc cattttgcgc cagttgtgtc gccgcctttc
     3601 ccgcgcccca caccagcaat acgaagacga attgcgccag aatgagtgca caaatgctgc
     3661 cttttttacc gggaataaaa caggccgtca gccaggcgca accaacgccc accagcatcc
     3721 agatcgtttg cggccacagc tgccagagat gacgcccctc acccgaaact aaacgattag
     3781 gcgcgtaact gataaacggc agcgccgctg cgattgccgt cagcaacagc agcagcgcca
     3841 gaacaggatt aatacggaaa taagtcacgg gaaattactt cgtccaccct ttttgtttca
     3901 ggtagtcggc agccactttt ttggcatcca gtccttccac agcaatgctg gcattcagtt
     3961 gctgcaatgt ttttgcatcg aggctggcga agactggctg tagccactgt gccatttgcg
     4021 gatactcctt caacaccgac tcacgcacca ctggtgcagg cgcgtagata ggttgcacac
     4081 cttgcggatc gcttaaggtt tgcagcccca gcgccgctac cgggccgtca gtgccgtaag
     4141 ccattgcagc attaacgcca gagatttgct gggcagcggc tttgatcgtc accgccgtgt
     4201 cgccaccagc cagtgacagc aactgatcct gaccgagctt aaagccatag gctttttcaa
     4261 acgcgggtaa cgcatcggcg cgttcgataa actctgccga ggctgccagt ttgaaggtgc
     4321 cgccctcttt cagataacga ctcaggtcgg caagcgaagt gagtttattt ttctctgcca
     4381 catcctgacg cacggcgata gtccaggtgt tatttgcagg cgcgggcgtc agccagatta
     4441 acttgttgtg ctccgaatcg agctttttga ctttctcgta accttgctgc gcgtttttcc
     4501 acgctgcatc gttttcatct ttaaagaaga aagcgccatt gccggtatat tccggataga
     4561 tatccagttc accggaagta atcgccccgc gcaccacagg agtcgttccg agttgcactt
     4621 tattcaccgt cggtacgccg tggctttcca gcacctgcaa gatgatgttg ccgagtagcg
     4681 cgccttcggt atcgattttt gaaccgactt taacggggga agccgcctgt agcggcaggc
     4741 tcacggctgc caacataacc agtgaacctg cccagacctt tgagagtggc atgatgcttt
     4801 cctcattctt ttactgttgt tttcagcgaa ttaagagaaa agcatagttg ataatgacag
     4861 agttagcctg agcggaagga ttcagttgca gaatcagata aatacttaaa gggcatattt
     4921 tgcccggcgt ttcttgccgg atgcggcgta aacgccttat ccggcctaca aaggtcgaaa
     4981 attcagcaag ttaagatatg aacatcaggc aaagggcgca aacgtcgcgc ccttactaaa
     5041 gcattacagc aactcaaact cgcctttctt aacgcgtgcg gaatcagtgc cgataaagac
     5101 attgaacttg ccaggctcgg cgtcatattt catctgttga ttccagaact tcagcgcctc
     5161 gatatcaatc gggaagctga cagtctgagt ttcgcccggt ttcagggtga ttttctcaaa
     5221 gcctttcagc tgcttcactg gacggctcat ggaagccgtc acatcctgca agtacatctg
     5281 tactaccgtg gccccttcgc gtttaccggt gttcgtcacc tgcacgctgg ccgtcacttt
     5341 gccgtcacgc ttcatggtcg gcgcagaaag tttcacatca gagacggtga aagtggtata
     5401 gctcagacca tagccgaacg gataaagcgc cccgttagct tcatcaaaat aacgcgaagt
     5461 gtatttgttc ggcttgtcgg cattatacgg acgaccggtg ttcagatggc tgtagtacac
     5521 cgggatctgc ccgacagaac gcgggaagga catcggcagc ttgccggacg ggttgtaatc
     5581 gccaaacaac acatcggcaa ttgcattacc gccttcagtc cccgcaaacc aggtttccag
     5641 aatcgcatca gcctgctgat cttctttcac cagcgccagc ggacgcccgt tcatcagcac
     5701 cagcaccagc ggtttaccgg tggctttcag cgccgcaatc aagtcacgtt ggctttgcgg
     5761 aatagtgata tcggtacggc tggaggcctc gtgcgccatc ccctgtgctt caccgaccac
     5821 agccaccacc atatcagatt gtttcgccgt ctgcaccgct tcatcaatca tctcttgcgg
     5881 cgagcgcgga tcgactttga ccgcttcttc atactgattc aggaaatcga taatgccttt
     5941 gtcactggta acgttcgccc ctttggcata cagcacttta ccgttttcac cgacggcatt
     6001 tttaatcccg gtcagtacgg tcacggattg atcggcaaca ccggctgcgg accagctgcc
     6061 catcacgtcg cgtttactgt ccgccagtgg cccaaccacc gcaatggtgg ccgatttttt
     6121 cagcggtaac gtttcgagac ggtttttcag caacaccagg ctttcgcgcg ccacttcacg
     6181 tgcttcttta cggtgcaggc ggctttcggc attggtatcc accgggtcag actctttcga
     6241 ccccaaatgg ctgtatgggt cgttaaacaa tcccatatca tatttaacgt tcagtacatg
     6301 gcgggcagca tcgtccagct cttccatcgt cactttgccg gatttgatca acccaggcag
     6361 atacttcgag taatactcgt cgctcatact catgttgatt ccggatttca gcgccacgcg
     6421 caccgcatct tccgggtctg ccgccgtgcc atgtttaatc agctctttga ttgcaccgtg
     6481 atcggaaacg gtgatgcctt taaagcccca ctggtcgcgc agaacatctt tcagcagcca
     6541 ggaatcggag gtggctggcg tgccgttcag cgagttcagc gccaccatca ccgcgccgct
     6601 gcctgcgtcc agccccgctt tgtacggcgg catataatca ttaaacaggc gctgcggact
     6661 catatcgacg gtgttgtact ctttaccgcc ttctaccgcg ccgtatgcgg caaagtgttt
     6721 gacgctggtc atcaccgagt agcgatctgc cgggctttta ccctgcatcg cttccaccat
     6781 ggttttaccc attgttgagg tgagatacgt atcttcgcca aaaccttcgg aagcacgtcc
     6841 ccagcgcgga tcgcgcgaga catcgaccat cggtgcccag gtcatattca ggccatcatc
     6901 tgccgcttca taagcagaga cacgtccgac cgttttcact gcatcgaggt taaaagacga
     6961 ggccagaccg aggctaatcg ggaacaccgt gcgctgaccg tgcagcacgt cgtaagcaaa
     7021 gaaaagagga attttcaggc ggcttaattc catcacctga tcctgcatgg cgcggatatc
     7081 ctgacgggtt acggtgttga aaatcgcccc aacctgaccg tctttgatca tctcgcggat
     7141 cgcctctttc gggttatccg ggccgacgct gattaagcgc agctgaccaa ttttctcatc
     7201 aactgtcatt ttcttaagca attcggtgac gaacgcatcc cgcgcttcgg gcgtgaatgg
     7261 atggttgccg aataaatcat ccgccagtgc tggctgcagg gccagactca ccgcgattcc
     7321 tactgaacat agccatttca tatggatttt tcttcctctg ttgcccgacg aggcagcgaa
     7381 aatggtgcaa aaaccgtagt ttgccataag catgatggag agagaaaaag aatgctcagt
     7441 ttattgtctg aattttcaaa atattcactc gctgaattgt catacaaggc gctattctag
     7501 tttgtgatat tttttcgcca ccacaaggag tggaaaatgt cttccatgac aacaactgat
     7561 aataaagcct ttttgaatga acttgcccgt ctggtcggtc attcacacct gctcaccgat
     7621 cccgcaaaaa cggcccgcta tcgcaagggc ttccgttctg gtcagggcga cgcgctggct
     7681 gtcgttttcc ctagctcact actggaattg tggcgggtgc tgaaagcctg tgtcaccgcc
     7741 gacaaaatta ttctgatgca ggctgccaat acaggcctga ccgaaggatc gacgccaaac
     7801 ggtaacgact atgatcgcga tatcgtgatc atcagcaccc tgcgtctcga caagctgcac
     7861 gttcttggca agggcgaaca ggtgctggcc tatccgggca ccacgctcta ttcgctggaa
     7921 aaagccctca aaccgctggg acgcgaaccg cactcagtga ttggatcatc gtgtataggc
     7981 gcatcggtca tcggcggtat ttgtaacaac tccggcggct cactggtgca acgtggcccg
     8041 gcgtataccg aaatgtcgtt attcgcgcgt ataaatgaag acggcaaact gacgctggtg
     8101 aaccatctgg ggattgatct gggcgaaacg ccggagcaga tccttagcaa gctggatgat
     8161 gatcgcatca aagatgacga tgtgcgtcac gatggtcgtc acgcccacga ttatgactat
     8221 gtccaccgcg ttcgtgatat tgaagccgac acgcccgcac gttataacgc cgatcctgat
     8281 cggttatttg aatcttctgg ttgcgccggg aagctggcgg tctttgcagt acgtctagat
     8341 accttcgaag cggaaaaaaa tcagcaggtg ttttatatcg gcaccaacca gccggaagtg
     8401 ctgaccgaaa tccgccgtca tattctggct aacttcgaaa atctgccggt tgccggggaa
     8461 tatatgcacc gggatatcta cgatattgcg gaaaaatacg gcaaagacac cttcctgatg
     8521 attgataagt taggcaccga caagatgccg ttcttcttta atctcaaggg acgcaccgat
     8581 gcgatgctgg agaaagtgaa attcttccgt ccgcatttta ctgaccgtgc gatgcaaaaa
     8641 ttcggtcacc tgttccccag ccatttaccg ccgcgcatga aaaactggcg cgataaatac
     8701 gagcatcatc tgctgttaaa aatggcgggc gatggcgtcg gtgaagccaa atcgtggctg
     8761 gtagattatt tcaaacaggc cgaaggcgat ttctttgtct gtacgccgga ggaaggcagc
     8821 aaagcgtttt tacaccgttt cgccgctgcg ggcgcagcaa ttcgttatca ggcggtgcat
     8881 tccgatgaag ttgaagacat tctggcgttg gatatcgctc tgcagcgtaa cgacaccgag
     8941 tggtatgagc atttaccgcc ggagatcgac agccagctgg tgcacaagct ctattacggc
     9001 cattttatgt gctatgtctt ccatcaggat tacatagtga aaaaaggcgt ggatgtgcat
     9061 gcgttgaaag aacagatgct ggaactgcta cagcagcgtg gcgcgcaata ccctgccgag
     9121 cataacgtcg gtcatttgta taaagcaccg gagacgttgc agaagtttta tcgcgagaac
     9181 gatccgacca acagtatgaa tccggggatc ggtaaaacca gtaagcggaa aaactggcag
     9241 gaagtggagt aaaaattacg gatggcatag tatcgccatc cgtcattcac ttaatcgttc
     9301 tgtgccgtct gccccgccgc cgccatttgg gcggcttttt gttttttata gctcaacgct
     9361 gctgctggaa caggcatcac tttaccggtt tcaatccagg tacgcaggcg gctggcatcg
     9421 gcaaaatggg tatatttgcc aaacgcatcc atcactacca gcgccaccgg tttattattg
     9481 ataaccgtac gcatcaccag acaatggccc gccgcattgg taaaaccggt tttggttaac
     9541 tgaatattcc agttatcgcg atacaccaga tggttagtat tgcggaacgg cagcgtatac
     9601 gtcgggttag agaaggttgc catatcttcc cgagtagtac ttaactgccc gatcaacgga
     9661 tattgtttgc tggcaatgag cagtttggtt aagtcacggg cagttgaaac gttatgcacc
     9721 gacaatccgg taggttcaac aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca
     9781 ttcattgcct taataaaggc tttgtaacca ccgggataat ggtgtgcaag gcttgccgcc
     9841 gcgcggtttt ctgaagacat cagcgccagc aacagcatat ctttacggct gatttcgcta
     9901 ttcagtcgta cgcgcgaata gacccctttc atctccggcg tctggctgat atccactttt
     9961 agtttttcat ccagcggcag tcgtgcatcc agcacaacca tcgcggtcat taatttgctg
    10021 atagacgcaa tcggacgcac cagatccggg tggttcgagt agatcacttt gttggtattc
    10081 agatcaacaa tcatcgcgct accggaggca atttcaggtt gtgaagcggt gatagcggct
    10141 gccgttttcg caacggcctg cggtgccaaa ggcacagcca gcatcagggc caggctaaat
    10201 aaagaaactc gaaatttcgg catgatgagc attcagatag tggttcacgc gcacaggttg
    10261 cgcaccgccg gagtaaggat ttactgaggc tagcgacgcc atcataacga gcaaaaagtg
    10321 cgatcgtcaa aggagaatcg tgaggaaatg ctgcattgct gacatttacg ccagcaatgc
    10381 aacgtcaaag aactttctag aacaaacgat aaccgtagcc ccatagtata acggttaggg
    10441 cgagcagcac ttccagtacc agcacgccaa tcgccagcgt cgaactagag aagctaaggc
    10501 cttcctcttt gttgatattc aggaagctcg gaataccaag gtaaagcagg tagccggtgt
    10561 aaaacagcgc caccgtgccg accagcgcac acaaccagac cagtggataa agcgccacca
    10621 aaccgcttaa aaacagcgga gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg
    10681 acggacgctg cggataatta cgcgccatcc accagatgac ccgccccatt accgcaaccc
    10741 cagccagcat aacgccataa aacaggacag ccagcgccag tccggtaaac caggataact
    10801 tcaggatagt gccatcgcca aaattccagc caatctgtgt agtgccaatg aaggcgcaaa
    10861 tcaccggaat cgccgccatc agcaaaacgt ggtgggtgta atggtgagaa atcgtttcgt
    10921 tttcgcgatt aatcacctgc atttcacgat cgggatggga aaacagtccc cagacatggc
    10981 tcataccgcc cccttgttgt gagttcatga acctgacagt tcaagtataa gtcagcttgt
    11041 gattattttt tgttcgctca tgtaatttgc accgtctgat gtacgtcctt tttcagcgtg
    11101 aatgatttcc agggtgtatg cttacaggca accaaaggga gacagactgg cctatggatc
    11161 tcaatacact catctcacaa tatggttatg ccgcgctggt gatcggtagc ctggcggaag
    11221 gtgaaaccgt gactttgctg ggaggcgttg cggcacatca ggggctatta aagttcccgc
    11281 tggtggtact ttctgtggcg cttggcggca tgattggcga ccaggtgctt tatctgtgcg
    11341 ggcggcggtt tggcggcaag ctgttacgcc gtttctcgaa acatcaggat aaaattgagc
    11401 gggcgcagaa acttatccaa cgccatccgt atctgtttgt cattggtacg cgctttatgt
    11461 atggctttcg ggtgattggc ccgacgctga ttggtgccag ccagctgccg ccgaaaatct
    11521 ttctgccgct gaatattctc ggcgcatttg cctgggcgtt gatttttacc actattggtt
    11581 acgctggtgg tcaggtgatt gcgccgtggt tgcacaatct cgaccagcat ttgaagcact
    11641 gggtctggtt gattctggtc gtggttctgg tggtgggcgt gcgctggtgg ctgaaacggc
    11701 gcgggaagaa aaagccggat catcaggcgt aaaaccattg ccctgggtaa ggcgttcgcg
    11761 ccgcatccga catccggtgc accatgcctg atgcgacgct tgtgcgtctt atcatgccta
    11821 ccaaaatttt acccattgaa ataaccacgc acctcactgc gctattctgg gttgaactgc
    11881 ggattcgcca acataaagcc gccatccact atcaacgact gcccggtggt gtaatttgcg
    11941 ccctctgagc acagccacgc taccaggctg gcaatctcat gcgttgcgcc aaaacgccgc
    12001 agaggaatcg aaggctccgc gtcgggcttc acgtcgctgt catccatgcc attcattggc
    12061 gtggcgatcg ccccaggcgc gactgcgttc accaaaatct tatgcctgac cagctccagc
    12121 gccatcgctt tggttaaccc accgagcgca tgtttagcgg ctgtgtaggc gctggcatcc
    12181 ggcagcggcg tatgttcatg taccgatgta atgttgatga tgcgaccgcc ctgcccttgt
    12241 ttcaccatct ggcgggccgc aatttgtgag cataaaaatg caccatcgac atcaacggta
    12301 aaaatcttgc gccactcatc gaaagccata tcaagaaacg gcgctttggt cattgcgccc
    12361 gcattattca ccagcacatc aatacgcccc agccgttgaa tgagtttctc cagcgcctgc
    12421 gcaccttctg gcagtttacc gagatccagc tgcacgatct ccgcacgtac gccgtggcta
    12481 actacctcac gcgcggtatc ttttgcccct tcttcatctg agtgccaggt aatgccaata
    12541 tcaaaccctt gctgcgccag taataacgcg cactctttgc cgatccccga atcggaggcg
    12601 gtaataatcg caacctgtgc catcgagttc tccacttaac gctgaataaa cgttaagtat
    12661 agaaggcgca tatcatcagc gtttgtaacc cccgcccaac gcaccagtga gttgaatgga
    12721 ggcatccagc cactgccctt gcaataacag gccattggcc cgctcacgca gcgcggggat
    12781 tctggcttcg ctgacgcggg aaccagcaat gatgcccgcg ttaaagcgcg cctgcgcaag
    12841 acctaccaca cgcaaggtat cgcgctcaat ttgcgcctga tgctggtttt tctccgcaag
    12901 tgtctgaacc tgactggctg cccgcgccac gtcattcacc gcttcaacca ccgctttgtt
    12961 gtagctggcg atagacaagt tgctttcggc ttttgcgata tcgagattgg cgttaagacg
    13021 accactatcg aaaatgggta gcgtcaggcc tgccgtaacg cccatttgct gcgcggaatg
    13081 acggaacaga tcgcttaagt gcaacgcatc ctgttgcagg aaggccatca ggttgatgtc
    13141 aggataaaat gccgcttttg ccgcatcaat ggtgcttagc gatgactcaa cgtaccagtg
    13201 cgccgcctgc aaatctgccc gccgggccag taaggagtac cccagttcat caggaagctg
    13261 gcttgccact ttcggcaacg cgaccggttt aagcttcaat gactttgtct ggttatttgt
    13321 aagtgcgctt aaccgtgcct caat
//
LOCUS       DAJTOR010000108        13343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTOR010000108 DAJTOR010000000
VERSION     DAJTOR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.108"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(102..3323)
                     /gene="carB"
                     /locus_tag="OO249_002777"
     CDS             complement(102..3323)
                     /gene="carB"
                     /locus_tag="OO249_002777"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5637014.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3341..4489)
                     /gene="carA"
                     /locus_tag="OO249_002778"
     CDS             complement(3341..4489)
                     /gene="carA"
                     /locus_tag="OO249_002778"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5637015.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(4944..5765)
                     /gene="dapB"
                     /locus_tag="OO249_002779"
     CDS             complement(4944..5765)
                     /gene="dapB"
                     /locus_tag="OO249_002779"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5637016.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(5932..6846)
                     /gene="rihC"
                     /locus_tag="OO249_002780"
     CDS             complement(5932..6846)
                     /gene="rihC"
                     /locus_tag="OO249_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5637017.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(6912..7862)
                     /gene="ispH"
                     /locus_tag="OO249_002781"
                     /gene_synonym="lytB"
     CDS             complement(6912..7862)
                     /gene="ispH"
                     /locus_tag="OO249_002781"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5637018.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(7864..8313)
                     /gene="fkpB"
                     /locus_tag="OO249_002782"
     CDS             complement(7864..8313)
                     /gene="fkpB"
                     /locus_tag="OO249_002782"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5637019.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(8382..8876)
                     /gene="lspA"
                     /locus_tag="OO249_002783"
     CDS             complement(8382..8876)
                     /gene="lspA"
                     /locus_tag="OO249_002783"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5637020.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(8876..11692)
                     /gene="ileS"
                     /locus_tag="OO249_002784"
     CDS             complement(8876..11692)
                     /gene="ileS"
                     /locus_tag="OO249_002784"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5637021.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(11735..12676)
                     /gene="ribF"
                     /locus_tag="OO249_002785"
     CDS             complement(11735..12676)
                     /gene="ribF"
                     /locus_tag="OO249_002785"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5637022.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYVVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(12684..12902)
                     /locus_tag="OO249_002786"
     CDS             complement(12684..12902)
                     /locus_tag="OO249_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5637023.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            13005..13268
                     /gene="rpsT"
                     /locus_tag="OO249_002787"
     CDS             13005..13268
                     /gene="rpsT"
                     /locus_tag="OO249_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5637024.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3108 a         3773 c         3369 g         3093 t
ORIGIN      
        1 aaaagattta cttaatgggc aaaaaaaacg gtcctcatca gagaaccgta ttacttattc
       61 gatcaaatta gcggatgaaa aatatccgcc atgacacgct attatttgat ctgcgcgtgc
      121 atttcctgca ccgaaattac tttttcggtc gcgtcggcat tcagcgccat cgcggtagcg
      181 aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc
      241 acgcgggagt cttcaatcgc gcgacggcct gaagtggtgt tgatgatgta ggtatattcg
      301 ccattcttga tgcggtcctg aatgtgcgga cggccttcat gcaccttgtt caccagacgc
      361 ggattaatgc ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag
      421 ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg
      481 gaaagcaacg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggca
      541 aacgcttcag cgaaggtgcg gcccacgccc atcacttccc cggtggagcg catttctggc
      601 cctaacagcg ggtcaacacc cgggaattta ttgaacggca gcaccacttc tttcaccgag
      661 tagtacggcg ggataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc
      721 acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacggta
      781 cgcgccgcac gcgggttaac ttcaatcagg tagacttcgt tgtttttcac cgcaaactgc
      841 acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc
      901 acatcctgaa tttcctgact taaggtgtag gctggcagag aacatgcgga gtcaccggag
      961 tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg
     1021 cagatggcat ccacgtcaac ttctaccgcg tcatcaagga agtgatccag caacactggc
     1081 gcatcgttag acacgctgac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag
     1141 acgatttcca tcgcccgacc gccgagaacg taagacggac gtaccaccag cgggtagcca
     1201 atctctttcg ccttctctac cgccatttca atagcggtaa cggtggcgtt cgccggttgt
     1261 ttcagtttca gacgctcaac cgcatgctgg aagcgttcac ggtcttctgc acggtcgata
     1321 gcatccgggc tggtgccgat aaccggtacg ccagcagctt ccagcgcgcg cgccagtttc
     1381 agcggggtct gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt
     1441 tccagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg
     1501 gtggagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcgcgcagc
     1561 gccagcgagg cgtgtacaca gcagtagtcg aattcgatac cctgaccgat acggttcggg
     1621 ccgccgccga gaaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct
     1681 tcataagtgg agtacatgta agcggtgtcg gtggcgaact ctgccgcaca ggtatccacg
     1741 cgcttataaa ccgggtgcag gtcatactgg tcgcgcagct tgcggatttc cgcttcgcgc
     1801 acgcccgcca gttttgccag acgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg
     1861 aattcagcgt tcaggccagt gatgcccact tccgctactt tctcttccag acgcaccagt
     1921 tcttcaatct gtaccaggaa ccagcggtca atattggtca ggttgaacac gccgtccaca
     1981 gacaggcccg cacggaaggc atcggcgatg taccagatac gctctgcgcc agcatctttc
     2041 agttcgcgac ggattttggt taacgcttcc gggtcatcga ggctcacttt cgggtcgaat
     2101 ccagtcgcac cgacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga
     2161 ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcacca
     2221 gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg
     2281 aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtcgag ggtgtaaccc
     2341 accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc
     2401 gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc
     2461 accgcaaact ggacattgga accaccggtt tcaacaccga tttcacgcag caccgccatc
     2521 gaggcgttac gcatgatttg atattctttg tcggtcagcg tttgggctgg cgcgacagtg
     2581 atggagtcac cggtatggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg
     2641 cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac
     2701 tcatcaatca gcagctcttt ggttggggag agatccagac cgcgggcgca aatttcttca
     2761 aactcttcgc ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga
     2821 ataatgcacg ggaagcccac gtcagcggca accgccagcg cttcttccat cgtgtgtgcg
     2881 ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg
     2941 tcttctgctt tatcaatcgc atcggcggtc gcaccaatca tagtcacgcc gaactcttcc
     3001 aacacgccct gacgctccag ctccagagcg cagttcagcg ccgtctgacc gcccatcgtt
     3061 ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtactacttc ccagtgaatc
     3121 ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag
     3181 ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag
     3241 tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt
     3301 tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt
     3361 actgctcaat taactcgata aagtggtcga acaacggcgc tgcgtcgtgt ggcccagggc
     3421 tggcttccgg gtgaccctgg aagctgaacg ccggtttatc agtgcgatga atgccctgta
     3481 acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat
     3541 ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa
     3601 ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca
     3661 gcgccagcag ctgatgaccg aggcagatgc cgaataccgg aatatcggtt tcgaggaatt
     3721 tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga
     3781 agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg
     3841 tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat
     3901 aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca
     3961 acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat
     4021 ccatgccatt cagacctggg aacgcgcggg ctttttctaa cgccaacgcc gcatccgggt
     4081 tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct
     4141 tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt
     4201 cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg
     4261 catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat
     4321 aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg
     4381 tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac
     4441 cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa acaccctcca
     4501 gagaatattc actcacttta tttgcatatt aattcacaat gatgacgtaa atcaatgcaa
     4561 atctgcttac cgacgaattt ctggcaaacg gcggcattct ggagatatgg gcgctaaaag
     4621 tcaacttaat ggcgggattt tttatattat tttgtggctc atgcatgttt tacaggcatt
     4681 aagcgacaaa aagatcatat aacctgttct ggaaaacgct tgcacagcaa gaagattgca
     4741 tgaaataaaa gcgagcaggg aaaagtggac caaatggtca aattttacat taaaacaaca
     4801 aaaacagaag acaaaacatg taatttttgg cacttataga caatttaaaa acataacact
     4861 aaaaataaaa gggccaaaat taaagccctt tataatcaat aaattaaatg tgttattttt
     4921 gcaccataac aaatattttg tggttataaa ctattgagat caagcacatc tcgcatatca
     4981 aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcgcc gttagcaaat
     5041 gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
     5101 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtgccaggc
     5161 acacgttcac cggtgtggct ttcacgactg tagaccgcgc aatctttcag atctttatca
     5221 agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
     5281 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct
     5341 ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc
     5401 gcaatatcgg cggcagcgtc acgaatcgcc tgtttaccgg cttcgtcaaa ccccgtagta
     5461 ccgatcacca tccctttgcc atgctggcga caaaaagcga gatggttcag cgtaccttcc
     5521 ggacgggtaa aatctataaa cacatcaaaa tcatctttta ccgcatcgag gctgctttgc
     5581 acggtaacgc ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa
     5641 gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag cgccgcctga
     5701 atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca
     5761 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
     5821 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
     5881 agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag gttacgacgc
     5941 cagagccagc acctcagcca cccactgctg gaagcctttc acatccagat ccaatgccac
     6001 ctttacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt
     6061 aaattcgccc tgagtttcca ctgccacaaa acacggtttg agagtgaaca attccgggcg
     6121 caccagccag gcaatagcgc aaagatcgtg catacgcagt ccactttgca tactgccgct
     6181 acggtagtgg ctaaacaggg cgtggagcat ttttccggtg cgattcaact ccggcaaagt
     6241 agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc tcatgacgat
     6301 ttcgatgccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc
     6361 ggcgtttggt gtgcagttgc cgcgaccagc agaaccgccc atgatcacca gacgacgaat
     6421 atacggttta cattccgggc attgtgaaag taacagcgca atattggtta acgggccgat
     6481 ggccaccagg gtaacgggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc
     6541 tggtatcccg agcggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc
     6601 gccgtgcaca gatgccgcat cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc
     6661 gagcggaatc tccgcattcc agaaatgcag cagttgcagg gcgttgcggg tggttttctc
     6721 aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gtgcaaaaat
     6781 cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa
     6841 atgcatgttt tctccataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg
     6901 ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa
     6961 acaatgtttt cttcacggcc ttccagcgga atggcttcac caccgcccag ctgctgcaaa
     7021 cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg
     7081 catttaacct ctttcaccca ctcttcctgg atgtctttcg catcgtcaat caaaaacgcg
     7141 cgtttgccca tacgctgggc cagctccgcc agacggttgg agttggagga gtttttcgaa
     7201 ccgaccacca acacaacttc cgcctgttct gccagggcgc gtaccgcttc ctgacggtta
     7261 gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc
     7321 agcgcgtcga tcacatcaga cgtgtcatcc accgacagcg tggtctgggt cataaaggag
     7381 agcttctctt cgtttttgac cgtcagtttc cacacatcgt ccggcgattc gaccagatac
     7441 attccccctt ccgggttact gtactggccc attgtccctt ccacttccgg gtgcccggcg
     7501 tgaccgatga gaatagattc ttcgccacgg cgactggcgc gggcgacttc catatgcact
     7561 ttggtcacca gcggacaggt cgcgtcaaat accgtcaaat cgcgactttt tgcttcgtta
     7621 cgtaccgcct gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggtacttcg
     7681 ctaatctgct caataaagat agccccacgc tcacgcaagc tatcgaccac atagcggtta
     7741 tgtaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca
     7801 atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc
     7861 atgttacgcc tccaatgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg
     7921 cccggccaga ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac
     7981 gccaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc
     8041 gcctgcatcc ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc
     8101 aaacgccgca tctggctcca acgagaaggt ggttttatcg cccactttca gccccaacag
     8161 gtgttgctcc agcccttcag aaagagaagc atcacccagg cggaacagcg ccggtttacc
     8221 gttgttgcgg gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag
     8281 gacggcgcta ttgctctgta cagattcaga catgcaggtt tgctcttata tttgaggatt
     8341 tttgccggat gcggcgtaaa cgccttatcc ggcagggttt attattgttt tttcgctcta
     8401 gaaggcaaaa aaccttccag cacaatcagt gccgcaccga cacagatggc agtatcggca
     8461 aggttgaagg tggcgaagtg ccagtcgccg acgtagaagt cgatcatatc gacaacgaag
     8521 ccgtgccaca ggcggtcgaa caggttgccc agcgcgccgc caataatcag cgcgtaagcg
     8581 atattgttta gcttctgcgt ggccttcgag cgatacatca tcactgccag gatcacgcta
     8641 ataccaatcg caataccggc aaagaaccaa cgctgccagc cgccgctatc ggcaaggaaa
     8701 ctaaacgccg cgccatagtt acgcgcataa tgcagattaa gcgacgggaa cagcgggacc
     8761 gtatccccca gagcaaagtt ctggaggatc aggtatttgc tgcccagatc gataatcagc
     8821 acgactacca ccagccacag ccagcgtagc cctgttgaac agatcgattg actcatcagg
     8881 caaacttacg tttttcaccg tcaccggcga cgttgctgac acagcggccg cagatttctg
     8941 cgtgttccgc caccttgccg acatcctggg tgtagtgcca gcagcgtggg cacttctcac
     9001 cttcggcttt actcaacgcg actttcagcc ctttgagtac ttcgctctgc tgagcatcag
     9061 caggtgcgtc gtgatagtct gcaacggtag cgccggaggt caacaggaca aatcgtaatt
     9121 catcgcccag cgcggtcagt ttcgccgcca gttccggttc tgcatacaag gttactgccg
     9181 cttccagcga gccacccact ttcttgtcgg cacgcgcttg ctcaatgact ttgttcactt
     9241 cgccacgcac tttcaacagc tcgtcccaga acgcatcgtt catcgcttcg ctgtctgcca
     9301 ggccaaacag gccttcgtac cactcaccgg tgaagacgta tttttcacgt tcgcccggca
     9361 ggtagcccca cacttcatca gcggtgaagg agaggattgg tgccatccag cgcaccagcg
     9421 cttctgcgat gtgatacagc gcagtctggc agctacgacg cgccacactg tccgctttgg
     9481 cggtgtactg acggtctttg atgatgtcga ggtagaagga acccatctca acggagcaga
     9541 agcgcatcag acgctgtacc acttcgtgga aatcgtatgc ttcgtacgcc ttgaggatgt
     9601 cttcctgtgc cgctttcgca caacctacgg cccagcgatc cagtaccacc atctcttccg
     9661 gtttcaccat atcttttgct ggatcaaaac cgttcaggtt tgccagcagg aagcgcgcgg
     9721 tgttacggat acgacgatag ctatcggcag cacgtttcag gatctcgtca gaaacggcca
     9781 tttcaccggt gtagtcggtt gatgccaccc acagacgcag aatatccgcg cccagtttgt
     9841 tcatcacatc ctgcggcgaa acggtattgc cgatggattt agacatcttg cggccctgac
     9901 catccacggt aaagccgtgg gtcagtacct gacgatacgg cgctttgcct ttcatcgcgg
     9961 tggagatcat tagggaagac atgaaccaac cacggtgctg gtcagaacct tccagataca
    10021 tgtccgctgc gtgaccggca aattccggac gcacgtcaac aacagaagag tgggtagatc
    10081 cggagtcaaa ccatacatcc aatgtgtccg gcactttcac gtactgatca gcttcatcgc
    10141 cgaggatctc tttcgcatca agatcccacc acgcctggat gccgtcaact tcaacgcgtt
    10201 ttgccacttc ttccattagt tcgagagtac gcggatgcag ctcttccgtg tctttgtgca
    10261 cgaacagtga catcggtacg ccccaggtgc gctgacggga gatacaccag tcaggacggt
    10321 tagcaaccat cgactcgata cgcgcctggc cccagtccgg gatccactgc acgcctttga
    10381 tctctttcag tgactgcgca cgcagacctt tctgatccat gctgacgaac cactgcggcg
    10441 tcgcgcggaa gatgatcggc gttttgtgac gccagcagca cggatagctg tgctgcattt
    10501 tctcaacgtg cagcagcgcg cctttttcct gcagcagcgc aacgacgatg tcgttcgctt
    10561 tgaagacgtt cacgccatcc agcgtcggat aagtgcccgg cagataagtg ccgtccgggc
    10621 caaccgggtt agcggtttcc aggccgtatt tctgaccgat cacatagtcg tccgggccgt
    10681 ggccaggcgc ggtgtgaacg gcaccggtac cggcatccag ggtaacgtga tcgccgagga
    10741 ttgccggaac gtcgaagccc ataaacggat gggtaaagcg cagcaactca agctccgcac
    10801 cttttaccgt gccgagaatg gtgtaatcgg tcacgccgat acgctgcatt acgctttcaa
    10861 ccagatcttt cgccagaatc acggcctgac cgtcgatctg caccagcgca tagtcgaaat
    10921 caggtgcaat agagattgcg cggttcgcag gcagagtcca cggcgtagtg gtccagatca
    10981 ccagcgagat tgggccgtta acgttgctta cggcaaattt tgctttcagt gcatcctgat
    11041 cgaccgcctg aaaagcaacg tcgatggacg gagaagtttt gtcgtaatac tcaacttccg
    11101 cttccgccag cgcagaacgg cagtcaacgc accagtgtac cggcttcgcg cctttgtgca
    11161 ggtgaccgtt gccgatgatt ttgcccagcg cgcggatgat gttggcttca gttttgaagt
    11221 ccatggtcag gtacgggtgc gaccagtcgc ccagcacgcc cagacggata aagtctttgc
    11281 gttgaccgtc aacctgggtc gccgcgtatt cgcggcactt ggcgcggaac tcggcggcgg
    11341 tgaatttctc acccggctta ccgtattctt gctctacttt cagctcgatc ggcagaccgt
    11401 ggcagtccca gccaggcaca tacggcgagt cataaccgga aagccctttg gacttcacga
    11461 taatgtcttt cagaatcttg ttaaccgagt gaccaatatg aatgctgcca ttcgcataag
    11521 gagggccatc atgcagaatg aaggtttttt tgcctttttt agccgcacgg atgatgccgt
    11581 acagatcatc atcagtccaa cgcgccagca ttcccggttc gcgcttggcg agatcgccac
    11641 gcatcgggaa ccctgtttcc ggcaaattca gggttgattt atagtcactc atcagattct
    11701 cggttccgtg tttcggtttg attacataac aggcttaagc cggttttgtt agcccaaaaa
    11761 attcgcgggc ggttaattca tcacgcgcaa tctgcgcttt cagttcgtcc agcgacgcaa
    11821 atcgctgctc attgcgtatt tttttacgca gcactacttg tatatggcga ccgtaaaggt
    11881 ccattgcaac atctaacaaa tgcacttcca gctgctggcg aataccggca accgttgggc
    11941 gtgttccgat gtttgccacg ccgggtaacg gcttttcacc gaggcccagc acttctacca
    12001 cataaacccc tttcaccggg gaaacctgac ggcgtagcgg tacattcgcc gtcgggaaac
    12061 ctatagtgcg ccctaattca tcaccgtgga ctacacgccc ggagatggca aacgggtgcc
    12121 ccagtaaact ttctgccaga gccagattgt catccgccag cgcctgacgc acagcggtgc
    12181 tgctaatacg cacgccacct tcgcaaaaag tttgcgtact agtgatatcg aagccgtatt
    12241 ccatgccagc tttctgtaat aacaagaaat cgccttcacg accagcgcca aagcggaaat
    12301 catcccctac ggcaagaaat tttacgcgca aatgcttcac cagaagatcg ctgatgaaat
    12361 tttgcgcggt taacgccgcg aaacgcctgt cgaaacgcac gcacagcacg taatcaacgc
    12421 cacactctgc aaggtaacgc agtttttccc gcagccgggt cagtcttgcc ggggctttat
    12481 cggtagcaaa cagttccagt ggttgaggtt caaaaagcat caccatcacc ggtaagttgc
    12541 gcttgcgccc ttcttcctgc aagccctgta acagcgcgcg atgaccgcga tgcacgccgt
    12601 cgaaattacc aatagtcagc acacaccctt cttgcggggc ctggctgaga ttatgtatgc
    12661 cgcgtatcag cttcatgtct ggctcaaaac agtgaaaatc gtccgagtat accttgtaca
    12721 gcggtcaagg ttaaccggcg attgagtacc gaaatcagaa agaagtggct tttcattgcc
    12781 atggcgcaaa tcacgggaag aaagtgaccg cctgctgcaa tttttatcgc ggaaaagctg
    12841 tattcacacc ccgcaagctg gtagaatcct gcgccatcac tacgtaacga gtgccggcac
    12901 attaacggcg cttatttgca caaatccatt gacaaaagaa ggctaaaagg gcatattcct
    12961 cggcctttga attgtccata tagaacacat ttgggagttg gaccttggct aatatcaaat
    13021 cagctaagaa gcgcgccatt cagtctgaaa aggctcgtaa gcacaacgca agccgtcgct
    13081 ctatgatgcg tactttcatc aagaaagtat acgcagctat cgaagctggc gacaaagctg
    13141 ctgcacagaa agcatttaac gaaatgcaac cgatcgtgga ccgtcaggct gctaaaggtc
    13201 tgatccacaa aaacaaagct gcacgtcata aggctaacct gactgcacag atcaacaaac
    13261 tggcttaatc gccaatttgc tgaagctttg tgaaaaagcc cgcgcaagcg ggttttttta
    13321 tgcctgctgc ttttatgatg cgt
//
LOCUS       DAJTOR010000109        13342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTOR010000109 DAJTOR010000000
VERSION     DAJTOR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.109"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            101..916
                     /gene="mepH"
                     /locus_tag="OO249_002788"
     CDS             101..916
                     /gene="mepH"
                     /locus_tag="OO249_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5637025.1"
                     /translation="MAQINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1044..1625
                     /gene="sodB"
                     /locus_tag="OO249_002789"
     CDS             1044..1625
                     /gene="sodB"
                     /locus_tag="OO249_002789"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5637026.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1771..2940)
                     /locus_tag="OO249_002790"
     CDS             complement(1771..2940)
                     /locus_tag="OO249_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5637027.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAAAFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3106..3195)
                     /gene="ynhF"
                     /locus_tag="OO249_002791"
     CDS             complement(3106..3195)
                     /gene="ynhF"
                     /locus_tag="OO249_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5637028.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3494..4519
                     /gene="purR"
                     /locus_tag="OO249_002792"
     CDS             3494..4519
                     /gene="purR"
                     /locus_tag="OO249_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5637029.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4516..5448)
                     /locus_tag="OO249_002793"
     CDS             complement(4516..5448)
                     /locus_tag="OO249_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5637030.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5561..6772
                     /locus_tag="OO249_002794"
     CDS             5561..6772
                     /locus_tag="OO249_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5637031.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            7063..8211
                     /gene="cfa"
                     /locus_tag="OO249_002795"
     CDS             7063..8211
                     /gene="cfa"
                     /locus_tag="OO249_002795"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5637032.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8251..8892)
                     /gene="ribE"
                     /locus_tag="OO249_002796"
     CDS             complement(8251..8892)
                     /gene="ribE"
                     /locus_tag="OO249_002796"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5637033.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9107..10480
                     /gene="mdtK"
                     /locus_tag="OO249_002797"
     CDS             9107..10480
                     /gene="mdtK"
                     /locus_tag="OO249_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5637034.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10521..11777)
                     /locus_tag="OO249_002798"
     CDS             complement(10521..11777)
                     /locus_tag="OO249_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5637035.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12085..12161
                     /locus_tag="OO249_002799"
     tRNA            12085..12161
                     /locus_tag="OO249_002799"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12119..12121,aa:Val,seq:gac)
     gene            12166..12242
                     /locus_tag="OO249_002800"
     tRNA            12166..12242
                     /locus_tag="OO249_002800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12200..12202,aa:Val,seq:gac)
     gene            12350..12655
                     /locus_tag="OO249_002801"
     CDS             12350..12655
                     /locus_tag="OO249_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5637036.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12781..>13342
                     /locus_tag="OO249_002802"
     CDS             12781..>13342
                     /locus_tag="OO249_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR5637037.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEA"
BASE COUNT         3289 a         3447 c         3173 g         3433 t
ORIGIN      
        1 ataaaagcta ttaactttct cttcttctat gcattagaat catcaagttt tgtaaatcag
       61 acgcaggcat gatagacctg ccttaacaga gggacgctca gtggcgcaga taaaccgtat
      121 ttcgatcacg ctctgtgctt tactttttac caccctgcct ttaacgccta tggcccatgc
      181 ttcaaagcaa gccagggaga gttctgctac cactcatatc accaaaaaag cagataaaaa
      241 gaaaagcacg gcaaccacca aaaagaccca gaaaacagcg aaaaaagccg ccagtaaaag
      301 tacgaccaaa agcaaaaccg cttcttccgt taaaaaatct tccattaccg cttctaaaaa
      361 cgccaaaact cgcagcaaac acgccgtcaa taaaacggcc tcagccagct ttaccgaaaa
      421 gtgtaccaaa cgtaagggct ataaatcgca ttgtgtgaaa gtcaaaaatg ccgcgtcagg
      481 aactcttgcc gacgcgcaca aagcgaaggt gcaaaaagct acgaaagtgg caatgaataa
      541 actgatgcag caaattggta agccatatcg ttggggtggc agctcaccgc gtaccggttt
      601 tgattgcagc ggcctggttt attacgctta taaagatttg gtgaaaattc gtattccgcg
      661 tacggcgaat gaaatgtatc acctgcgtga tgcagcgcca atcgaacgta gtgaactgaa
      721 aaacggcgac ctggtctttt tccgtactca gggacgcggc acagccgatc atgtcggcgt
      781 gtatgtcggc aacggcaaat ttattcagtc accgcgcaca ggtcaggaaa ttcaaatcac
      841 ctctctcagt gaagactact ggcagcgcca ctatgttggc gctcgtcggg taatgacccc
      901 aaaaacactt cgctaaaact ttaccctgtt gttacggcaa cagggtaagt tcatcttttg
      961 tctcaccttt taatttgcta ccctatccat acgcacaata aggctattgt acgtatgcaa
     1021 attaataata aaggagagta gcaatgtcat tcgaattacc tgcactacca tatgctaaag
     1081 atgctctggc accgcacatt tctgcggaaa ctatcgagta tcactacggc aagcatcatc
     1141 agacttatgt cactaacctg aacaacctga ttaaaggtac cgcgtttgaa ggtaaatcac
     1201 tggaagagat tattcgcagc tctgaaggtg gcgtattcaa caacgcagct caggtctgga
     1261 accatacttt ctactggaac tgcctggcac cgaacgccgg tggcgaaccg actggaaaag
     1321 tcgctgaagc tatcgccgca tcttttggca gctttgccga tttcaaagcg cagtttactg
     1381 atgcagcgat caaaaacttt ggttctggct ggacctggct ggtgaaaaac agcgatggca
     1441 aactggctat cgtttcaacc tctaacgcgg gtactccgct gaccaccgat gcgactccgc
     1501 tgctgaccgt tgatgtctgg gaacacgctt attacatcga ctatcgcaat gcacgtcctg
     1561 gctatctgga gcacttctgg gcgctggtga actgggaatt cgtagcgaaa aatctcgctg
     1621 cataataact gatggcaaat gcagcattgc ctgatgcgct tcgtttacca tgccaacgcg
     1681 gatgaggcgt taacagcgca ttcggcaaga taaatcgcac gttgtcagca actgtaacgc
     1741 agaaggttat ccttctgcgt ttttgtttaa ttagctgtta gcaacgcaaa ctgtttcagg
     1801 ttgttttctg gctgacataa acaccagcaa taatgccagt cccgcgacaa tcgctcccat
     1861 caccggcaca aagctgtatc ccagcccagc ggaaattacc gcaccaccag cagctgctcc
     1921 cagcgcattt ccaagattaa aggcaccaat attgactgat gaagacagac ctggcgcttc
     1981 actggcgaca cgcatcacgc gcatctgtaa cggcggtacg accgcaaagg ctgctgcgcc
     2041 ccacaccacc atgctaatag ctgcgccgaa ctcattgcgg gccaggaacg ggattgccag
     2101 cataatcacc atcaacaaca acaaaaagcc tttcaacgtg ccgttaactg aacgatctgc
     2161 cagtttgccg ccgggatagt taccgataga gaatccgaca ccaatcagca ccagcattgc
     2221 cgtgacgaac accggtgttg cgtgggtaat actttgcagt accggagaga tataggtgta
     2281 gagagtaaac attgcaccag ctcccagtac cgtcgtcagc aatgcagaca acacctgcgg
     2341 acgcattaat accgccagct cttttttcac ttcaggtcgt gcccctgcac cacctttagg
     2401 taatgagaag aacagactta ccattgaaat cactcccagc cccgccgttg ccagaaatga
     2461 catccgccag ccgatggttt cacccaacca ggtcgccgcc ggcacgccac cgatatttgc
     2521 cagggttaac cccataaaca tagtggcaac tgcgctggcc tgtttatgtt ttggctccac
     2581 gcttgcggcc acgactgaac ccaaaccaaa aaatgctccg tgattcaggc tggtcaaaat
     2641 gcgtgaaagc atcagggtca tataatccgg cgcgatggcg gaaagtacgt tgccgagcgt
     2701 gaaaattgcc atcaggaaaa tcaacgcact gcggcgggca cgatgagaaa gtagaagcgt
     2761 catcagcggc gcgccaacca ttacgccaac tgcataggca ctgattaaca ttccggcagc
     2821 gggaatcgag acatccacac cgcgcgcaat gacgggcaac aagcccattg gcgagaactc
     2881 cgttgtcccg ataccaaacg cgccaatcgc cagagccagc aacggatagt taattttcat
     2941 gccttatctc cacctcttcg cgtcattacg cgatattcat taaagtggcg aaagcatgac
     3001 agcaatcaca aaaaaatgaa aataacaaaa agagaaaaca cttttgccat tttgctaaca
     3061 aacaggaagg agatgcgagg gagaacgcgc tccctcgaga ggaaatcagt gcagcgcggc
     3121 agtcaaaccc acggccacga tcaaaccgag gacgataatc gttgttacca gtgaaaattt
     3181 aaggtcggtg ctcatcaagt tttctccttt tttattacca cacaaatagt gatattacgc
     3241 atttttacac actgtgatga aaaaatctcc cgtcatttat aatgataagt gtttttacca
     3301 cttccccttt tcggcaagat cggccaaaat tccacgctta cactatttgc gtactggcca
     3361 ttgacccctt cctgacgctc cgtgtcgttt ttccggcgta ccgcaacact tttgttgtgc
     3421 gtaaggcgtg taaaggcaaa cgtttacctt gcgattttgc aggagctgaa gttagggtct
     3481 ggagtgaaat ggaatggcaa caataaaaga tgtagcgaaa cgagcaaacg tttccactac
     3541 aactgtgtca cacgtaatca acaaaacacg tttcgtcgct gaagaaacgc gcaacgccgt
     3601 gtgggcagcg attaaagaat tacactactc ccctagcgcg gtggcgcgta gcctgaaggt
     3661 taaccacacc aagtctatcg gtttgctggc gaccagcagc gaagcggcct attttgccga
     3721 gatcattgaa gcggttgaaa aaaattgctt ccagaaaggt tacaccctga ttctgggtaa
     3781 tgcgtggaac aatcttgaga aacagcgggc ttatctgtcg atgatggcgc aaaaacgcgt
     3841 cgatggtctg ctggtgatgt gttctgagta cccagagccg ttgctggcga tgctggaaga
     3901 gtatcgccat atcccaatgg tggtgatgga ctggggtgaa gcaaaagctg acttcaccga
     3961 tgcggtcatt gataacgcgt tcgaaggcgg ctacatggcc gggcgttatc tgattgaacg
     4021 cggtcaccgc gaaatcggcg tcatccccgg cccgctggaa cgtaacaccg gcgcaggccg
     4081 ccttgccggt tttatgaagg cgatggaaga agcgatgatc aaggtgccgg aaagctggat
     4141 tgttcagggt gactttgaac ccgaatctgg ttatcgcgcc atgcagcaaa tactgtcgca
     4201 gccgcatcgc cctactgccg tcttctgtgg tggcgatatc atggcaatgg gcgcactttg
     4261 tgctgctgat gaaatgggtc tgcgcgtccc gcaggatgtt tcgctgatcg gttatgataa
     4321 cgtgcgcaac gcccgctatt ttacgccggc gctgaccacg atccaccagc caaaagattc
     4381 gctgggtgaa acagcgttca acatgctgtt ggatcgtatc gtcaacaaac gtgaagaacc
     4441 gcagtccatt gaagtgcatc cgcgcttgat tgaacgccgc tccgtggctg acggcccgtt
     4501 ccgcgactat cgtcgttaat cacccgttgc gggagtctct tccggctccc gcagccactc
     4561 cttattcagc gtctcactat cgccgagata ctcaagcagc caggttaacg caggcgacat
     4621 atcattttgc tgccatgtca gacaacatgc cgaatccggg aaggggtttt ccaattcaag
     4681 tgctacccac ttcccctcat taagccacgg tttggcgaaa tgtgttggca ccatccctat
     4741 gcataatcct gccgagatac aggttgccga tgattcccag tcaggcacga caactctttt
     4801 ttggttatcc agtaaccagg taatacgttt aggtagcgtt cgcgaggtgt cttcgcgcac
     4861 caacgacggc cagttgcgca acgtatcatc gctgaatggg ccatccatca acgccagcgg
     4921 gtggtggctg gcaacaacgc aactccagct tagcatcccc atatcccgga aggcataacg
     4981 accgcctacc ggaatcgcgc gtgttgcgcc aatcgccagt tccacgcgcc cgtcggaaag
     5041 cgcatcccag acaccgttga acacttcctg aaagacaaga agttcgacat catcaaaatg
     5101 gcgataaaaa tcaacgatca tctgccgtgt acgttctggc ctgacaatat tatccactgc
     5161 gatagctaac tgaccgcgcc agccgttcgc tatctgctga cattgctggc gggtgatctg
     5221 catttttttg acaacagagc gcccttcttt gagaaaccac gctccagcag cggtcagctc
     5281 cacatcacgg tgccgtcgtt caaagagcgg caccgccagc cactcttcca gctgacgcac
     5341 ggtatagctg accgcagaag gaacgcgatg cagctcctgt gccgcagcgc taaaactacc
     5401 attacgcgct accgcatcaa caacttcgag tgaatattct gaccacatag tctgcctgca
     5461 aaatttttga aatcagtcat caaatattac cgtttcacaa cactaatttc actccctaca
     5521 ctttgcggcg gtgtttaatt gagagattta gagaatatac atgcaacctg ggaaaagatt
     5581 tttagtctgg ctggcgggtt tgagcgtact cggttttctg gcaaccgata tgtatctgcc
     5641 tgctttcgcc gccatacagg ccgacctgca aacgcctgcg tctgctgtca gtgccagcct
     5701 tagtctgttc cttgccggtt ttgccgcagc ccagcttctg tgggggccac tctccgaccg
     5761 ttatggtcgt aaaccggtgt tattaatcgg cctgacaatt tttgcgttag gtagtctggg
     5821 gatgctgtgg gtagaaaacg ccgctacgct gctggtattg cgttttgtac aggctgtggg
     5881 tgtctgcgcc gcggcggtta tctggcaagc gttagtgacg gattattatc cttcacagaa
     5941 agttaaccga atttttgcga ccatcatgcc gctggtgggt ctatctccgg ccctggctcc
     6001 tctgttagga agctggctgc tggtccattt ttcctggcag gcgattttcg ccaccctgtt
     6061 tgccattacc gtggtgctga ttctgcctat tttctggctc aaacccacga cgaaggccgg
     6121 taacaatagt caggatggtc tgacctttac cgacctgcta cgttctaaaa cctatcgcgg
     6181 caacgtgctg atatacgcgg cctgttcagc cagttttttt gcatggctga ccggttcacc
     6241 gttcatcctt agtgaaatgg gctacagccc ggcagttatt ggtttaagtt atgtcccgca
     6301 aactatcgcg tttctgattg gtggttatgg ctgtcgcgcc gcgctgcaga aatggcaagg
     6361 caagcagtta ttaccgtggt tgctggtgct gtttgctgtc agcgtcattg cgacctgggc
     6421 tgcgggcttc attagccatg tgtcgctggt cgaaatcctg atcccattct gtgtgatggc
     6481 gattgccaat ggcgcgatct accctattgt tgtcgcccag gcgctgcgtc ccttcccaca
     6541 cgcaactggt cgcgccgcag cgttgcagaa cactctacaa ctgggtctgt gcttcctcgc
     6601 aagtctggta gtttcctggc ttattagtat cagcacgcca ttgctcacca ccaccagcgt
     6661 gatgttatca acagtagtgc tggtcgcgct gggttacatg atgcaacgtt gtgaagaagc
     6721 tggctgccag aatcatggca atgccgaagt cgctcatagc gaatcacact gatctatatc
     6781 gatatactta tacttaggct gctaacaaaa ttttgttgta tgattgaaat tagcggccta
     6841 tactaatttc gagttgttaa agctacgata aatattatgt ttttacgggg acaggatcgt
     6901 tcccgactca ctatggatgg tcatttcggc aagggttcct cctttccctc tgttctacgt
     6961 cggattatag actcgcggtt ttttctgcga gatttctcac aaagcccaaa aagcgtctac
     7021 gctgttttaa ggttctgatc accgaccagt gatggagaaa ctatgagttc atcgtgtata
     7081 gaagaagtca gcgtaccgga tgacaactgg taccgtatcg ccaacgaatt acttagccgt
     7141 gccggtatag ccattaacgg ttctgccccg gcggatattc gtgtgaaaaa ccccgatttt
     7201 tttaaacgcg ttctgcaaga aggctctttg gggttaggcg aaagttatat ggatggctgg
     7261 tgggaatgtg accgactgga tatgtttttt agcaaagtct tacgcgcagg tctcgagaac
     7321 caactccccc atcatttcaa agacacgctg cgtattgccg gcgctcgtct cttcaatctg
     7381 cagagtaaaa aacgtgcctg gatagtcggc aaagagcatt acgatttggg taatgacttg
     7441 ttcagccgca tgcttgatcc cttcatgcaa tattcctgtg cttactggaa agatgccgat
     7501 aatctggaat ctgcccagca ggcgaagctc aaaatgattt gtgaaaaatt gcagttaaaa
     7561 ccagggatgc gcgtactgga tattggctgc ggctggggcg gactggcaca ctacatggca
     7621 tctaattatg acgtaagcat ggtgggcgtc accatttctg ccgaacagca aaaaatggct
     7681 caggaacgct gtgaaggcct ggatgtcacc attttgctgc aagattatcg tgacctgaac
     7741 gaccagtttg atcgtattgt ttctgtgggg atgttcgagc acgtcggacc gaaaaattac
     7801 gatacctatt ttgcggtggt ggatcgtaat ttgaaaccgg aaggcatatt cctgctccat
     7861 actatcggtt cgaaaaaaac cgatctgaat gttgatccct ggattaataa atatattttt
     7921 ccgaacggtt gcctgccctc tgtacgccag attgctcagt ccagcgaacc ccactttgtg
     7981 atggaagact ggcataactt cggtgctgat tacgatacta cgttgatggc gtggtatgaa
     8041 cgattcctcg ccgcatggcc agaaattgcg gataactaca gtgaacgctt taaacggatg
     8101 tttacctatt atctgaatgc ctgcgcaggt gctttccgcg cccgtgatat tcagctctgg
     8161 caggtcgtgt tctcacgcgg tgttgaaaac ggccttcgag tcgctcgcta aaggctattc
     8221 tatcgccccc tctccggggg cgatttcaga tcaggcttct gtacctggtt gattcatggc
     8281 attttctcgt gccgccagca cacgttctac cgtatctacc actgcctgag tttgtggatc
     8341 aatttcaatg ttgacgcgtg cgccgagttt tttcttccca agagtcgtgc gttccagagt
     8401 ttccggaatt aaatggacgc aaaaacgcgt tggcgtgact tcgccgacgg tcaggctaat
     8461 accgtcgatg ccaataaatc ctttgtacag gatatatttc atcaactgac tatcctggac
     8521 tttaaaccag atctggcgat tattttccga ggttaatatt ttcgccactt cagcggtggt
     8581 cataatatga cctgacatta agtgtccgcc aatttcatca ctgaatttcg ccgcacgctc
     8641 aacgtttacc caatccccca cttttaaatc gccaagattg gtaatgcgta acgtttcttt
     8701 catcaggtca aaactgacat ggttgccgtt aatttccgtc acggtcaggc agcaaccgtt
     8761 atgcgccacg gaagcaccgg tttccaggcc gtccagcatg tggtcgggta actccaccac
     8821 atgcgtacga aaatttggtt tctcgtcaat cgacaccagt tttgcggtgc cctgtacaat
     8881 ccccgtaaac atacttacaa ctcctgaaat cagttaagac attctgttca gcacaatagc
     8941 aggtggcaaa cgcccttacc agtgaagggg taagaatgac tattttttca ctggagaatt
     9001 aataaatcct cgctacaata gactgaattt cccctgcttc ttctttttgc tgcccattca
     9061 ggcggctttt tagtctctca tataactaca aataaaaggt gttcacgtgc agaagtatat
     9121 cagtgaagcg cgtctgttat tagcattagc aatcccggtg attctcgcgc aaatcgccca
     9181 aactgcgatg ggttttgtcg ataccgtgat ggcgggcggc tatagtgcca ccgacatggc
     9241 ggcggtcgct atcggtactt ctatctggct tccggcgatc ctctttggtc acggactgct
     9301 gctggcatta acgccggtta tcgcgcaatt aaatggttcc ggtcgacgtg agcgcattgc
     9361 gcatcaggtg cgacaaggtt tctggctggc aggttttgtt tccgttctca ttatgctggt
     9421 gctgtggaat gcaggttaca ttatccgctc catggaaaac atcgatccgg ctctggcgga
     9481 caaagccgtg ggttatctgc gtgcgttgtt gtggggcgcg ccgggctatc tgttcttcca
     9541 ggttgcccgt aaccaatgtg aaggtctggc aaaaaccaag ccgggtatgg taatgggctt
     9601 tatcggcctg ctggtgaaca ttccggtgaa ctatatcttt atttatggtc atttcggtat
     9661 gcctgagctc ggtggcgttg gttgtggcgt ggctactgcg gcggtgtatt gggtcatgtt
     9721 ccttgccatg gtttcttaca ttaaacgcgc ccgctccatg cgcgatattc gtaacgaaaa
     9781 aggcaccgcg aaacccgatc ctgcggttat gaaacgactg attcaactcg gtttaccgat
     9841 tgcgctggca ctattctttg aagtgacact gtttgccgtc gtggctctgt tagtgtctcc
     9901 gctcggtatt gttgatgtcg caggacacca gattgccctg aactttagtt cactgatgtt
     9961 tgtgcttcca atgtcgctgg cggcagcggt aactatccgc gtaggttatc gtctgggtca
    10021 gggctcaacg ctggatgcgc aaaccgctgc gcggaccggg cttatggtgg gtgtctgtat
    10081 ggcaaccctg acggccattt tcacggtttc actccgggag caaatcgccc tgttgtacaa
    10141 cgacaatccc gaggttgtaa cgctggctgc gcatttgatg ttgctggcgg cggtatatca
    10201 gatttctgac tcaatccagg tgattggcag tgggattttg cgtggttata aagatacgcg
    10261 ttccattttc tatatcacct ttacagctta ctgggtgctg ggcttgccaa gcggctatat
    10321 tctggcactg accgatctgg tcgttgaacc tatggggcca gcaggcttct ggataggctt
    10381 tattatcggc ctgacatcgg cagccattat gatgatgttg cgtatgcggt tcctgcaacg
    10441 tctgccgtca gttatcattc tgcaacgagc atcccgctaa taaagacaag gcgcaacctt
    10501 cacgggttgc gcctgtgttt ttacgcaggc tggagcgttg cgccaatccc gtcttcgtct
    10561 ggctgtaatt tcagagcgtt gcacagagtt tgccgactgg catctgttat ctcaacgtgt
    10621 atatcaccgg taacttcccc tttcacattg atgaagtcag gtgagatttc tttcgcactg
    10681 gtttgcaggc aaatcgctcc ctctccgcgc aggcgtttca ctttcagttg cacatgcgtt
    10741 gccgcaccag tgatcagcca cagttttccg ccattcagat tgatgttacc aacaataaaa
    10801 gtgacaccgt caccttttac cgtactgccg tcgagaatca acacaccgcc ctgctgtaca
    10861 gtaatcgttg attgttgcag atctgcgcca tagccaataa tcattgaagc gccgtccagt
    10921 aatgcaacac tcccggtcag ttcaaccggt gcgcgatctt cctctacgac aaaattttcg
    10981 tccgcgctgg tgggtgacaa caggatcact gttccacctt gtattaccgc attttccagc
    11041 gtaccggcat gcacttcagc ccgaccaccc acattcacgg acaggttttg cgtcttaccg
    11101 gaactgtaga gctgaaggcc atccgtcccc agacgataaa tggatccatt ctcaacaatg
    11161 gtgtcagagt cttcaccacc gagattattc atggtgccac cagcaagcaa cgtatcactg
    11221 gctttcccgg ccagcataaa aacgccgccc tgattaatct cacaattgct ggcttcccca
    11281 cctggataca ccaacaattc accaccttca tctaccacga ccgctgaggt tgtcccatta
    11341 accaacaggc cgccctcttg atcgagaatg attttttccg cgcgatgtcc ttccagtaca
    11401 cgcaggttac cgccattttc cagcaacaaa ccgcaggcat aaccctgatc aacgctgaat
    11461 tcaccttcgg gatggcggcc attaaccgta gcgagcgtag agagactaat tgcgcctccc
    11521 tcggactgaa caatatctgt ggcaatggca ccgtcgctga ccgatagcgt gccattgtta
    11581 ccgtactgtg tgcatttggc gatcccaccg gttgccactt tcaaccagcc agcccccgtg
    11641 actacggtat tttcggctct tccgccagct tcaacaatca actcgccttc agtaagttgc
    11701 gtcgcgccaa tgacctcgcc ggtcttaaca atttgcacat tcccgtcgct catcaagttt
    11761 ttcgcatcag atcccatgat ttattccttt gctgcatctg tgtgccttta ttgctaccta
    11821 aatgtaaagg ctacggagga tttatccacg acagatttga gatggtggca aacaactctg
    11881 tttaaactct gatacacgaa ttattgggtt gtatcagatg taaatgcgat cctgaataaa
    11941 aatcgccctt gcaaatcaac aaaatatagc caatttgctt aaaagctctg caaacggtcg
    12001 aaatagcgca gaaaattacg ttttgcctct tgccaccttc ccactctgcc gctaatattc
    12061 gtccccgttg tcacctacaa cgttgcgttc atagctcagt tggttagagc accaccttga
    12121 catggtgggg gtcgttggtt cgagtccaat tgaacgcacc atcctgcgtc cgtagctcag
    12181 ttggttagag caccaccttg acatggtggg ggtcggtggt tcgagtccac tcggacgcac
    12241 cagattttct taatctggtc ttctcctttt tccctctgtt tcttctctgt atccaatacg
    12301 ttaaaagatt tacactgtct tcgtatgcgt tatcagaagg agaatcgcta tggcaacttt
    12361 gttacaactt cattttgctt ttaatggccc atttggcgac gcaatggctg agcagcttaa
    12421 accgcttgct gagtcgatta atcaggaacc tggttttctg tggaaggtat ggacagaaag
    12481 tgaaaagaac cacgaagccg gtgggatcta tctttttact gatgaaaaaa gcgcccttgc
    12541 ctatctggaa aaacatactg cccgacttaa aaatctcggc gttgaggaag ttgtcgccaa
    12601 agttttcgat gtcaatgagc cactgagtca aatcaatcag gcaaaactcg cctgacagaa
    12661 tttaatcaag ggcggttagc gcccttttca tccctgtctg aaatttctca aattctaaaa
    12721 atctcaacca aacttatctg ataacactaa attcgaaaga atgcgtacag gtaagtaaca
    12781 atgaaaaaaa ttgctattgt gggtgccggg cctacgggga tctacacctt attctcgctt
    12841 ctacagcaac aaactccact ttctatttct atcttcgagc aggctgacga ggccggtgtc
    12901 gggatgccat acagtgatga ggaaaactca aaaatgatgc tggcaaatat tgccagtatt
    12961 gaaataccgc cgattaattg tacgtatctc gaatggctac aaaagcaaga agccagccat
    13021 ctccagcgtt atggcgttaa aaaagaaacc ttgcacgatc gtcagttttt accgcgaatt
    13081 ctgctgggcg aatatttccg cgatcaattt ttacgattag tagaccaggc acgacagcaa
    13141 aaatttgcag tggctgttta tgaatcatgc caggttaccg atctgcaaat tacaaatgct
    13201 ggcgtcatgc tcgctacaaa tcaggattta cccagcgaga cgtttgattt agcggtgatt
    13261 gccacgggtc acgtctggcc tgatgaagaa gaagcaacca gaacgtattt tcccagtccg
    13321 tggtcaggcc tgatggaagc aa
//
LOCUS       DAJTOR010000110        13314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTOR010000110 DAJTOR010000000
VERSION     DAJTOR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.110"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..310
                     /locus_tag="OO249_002803"
     CDS             <1..310
                     /locus_tag="OO249_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5637038.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAILLIN
                     "
     gene            509..790
                     /gene="ppiC"
                     /locus_tag="OO249_002804"
     CDS             509..790
                     /gene="ppiC"
                     /locus_tag="OO249_002804"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5637039.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(877..2352)
                     /gene="ilvC"
                     /locus_tag="OO249_002805"
     CDS             complement(877..2352)
                     /gene="ilvC"
                     /locus_tag="OO249_002805"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5637040.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2502..3392
                     /gene="ilvY"
                     /locus_tag="OO249_002806"
     CDS             2502..3392
                     /gene="ilvY"
                     /locus_tag="OO249_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5637041.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(3389..4933)
                     /gene="ilvA"
                     /locus_tag="OO249_002807"
     CDS             complement(3389..4933)
                     /gene="ilvA"
                     /locus_tag="OO249_002807"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5637042.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4936..6786)
                     /gene="ilvD"
                     /locus_tag="OO249_002808"
     CDS             complement(4936..6786)
                     /gene="ilvD"
                     /locus_tag="OO249_002808"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5637043.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6851..7780)
                     /gene="ilvE"
                     /locus_tag="OO249_002809"
     CDS             complement(6851..7780)
                     /gene="ilvE"
                     /locus_tag="OO249_002809"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5637044.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7800..8063)
                     /gene="ilvM"
                     /locus_tag="OO249_002810"
     CDS             complement(7800..8063)
                     /gene="ilvM"
                     /locus_tag="OO249_002810"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5637045.1"
                     /translation="MMQYQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8060..9706)
                     /locus_tag="OO249_002811"
     CDS             complement(8060..9706)
                     /locus_tag="OO249_002811"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5637046.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9709..9759)
                     /locus_tag="OO249_002812"
     CDS             complement(9709..9759)
                     /locus_tag="OO249_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637047.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            complement(9846..9944)
                     /gene="ilvL"
                     /locus_tag="OO249_002813"
     CDS             complement(9846..9944)
                     /gene="ilvL"
                     /locus_tag="OO249_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5637048.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10296..11816
                     /locus_tag="OO249_002814"
                     /pseudo
     CDS             10296..11816
                     /locus_tag="OO249_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(11841..12179)
                     /gene="maoP"
                     /locus_tag="OO249_002815"
     CDS             complement(11841..12179)
                     /gene="maoP"
                     /locus_tag="OO249_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5637049.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            12298..13137
                     /gene="hdfR"
                     /locus_tag="OO249_002816"
     CDS             12298..13137
                     /gene="hdfR"
                     /locus_tag="OO249_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5637050.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(13233..13308)
                     /locus_tag="OO249_002817"
     tRNA            complement(13233..13308)
                     /locus_tag="OO249_002817"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13273..13275),aa:Trp,seq:cca)
BASE COUNT         3091 a         3600 c         3449 g         3174 t
ORIGIN      
        1 tgtcgtgctc aacggaaaaa ttaatggcaa cgcatgtatc gtcgttccat tatcaaccac
       61 tcgcgatcat gacaagttaa atcgtggaat gcatgttgaa atagccagta atgttatcaa
      121 tggcctgcaa ttctttgatc agcagattcg ttgggccaaa gccgacctgg tacaacaagt
      181 cagtcgaaac cgattaaata gagcaaggac ttacagaggg tatcttaatc aatgtttgcc
      241 acatgagttg gttgcagaca tacagagggc ggtcatcaag tcaatcaatg ccatattgct
      301 cataaattga caataaaata accatccccg ataatgactt tgtagcccat agaggcccta
      361 agttttctcc ccttgaggga cctgcgaagc ccggattttc cgggcttttg ccatatatgt
      421 accagcccct ccctgactga caattacgtt agcatctgcg gatcatgcta aaatcgccgc
      481 ctgacaatta ttcactcaag gcacgatcat ggcaaaaaca gcagcagcac tgcatatcct
      541 tgtaaaagaa gagaaactgg ctctggatct tctcgagcag attaagaacg gtgccgattt
      601 cggcaagctg gcgaagaaac actccatttg cccatcaggc aaacgcggcg gtgatttagg
      661 tgaattccgc cagggtcaga tggttccggc gttcgataaa gttgtgttct cttgtccggt
      721 actggagccg accggcccgc tgcacaccca gttcggttac cacatcatta aggtgctgta
      781 ccgtaactaa tagcaaggcc ttctccagga gaaggccttg agtgttttct ccctctccct
      841 gtgggagagg gtcggggtga gggcatcagc gcgcacttaa cccgcaactg caatacgttt
      901 catatccgtc atatagccgc gcagtttctt acctacctgc tcaatcgcat ggcagcgaat
      961 cgcttcgttc acatcacgca gttgcgcgtt atctaccgca ccttccggaa tagctttacc
     1021 cagatcgccc ggttgcagct ctgccataaa cggtttcagc aacggcacac aagcgtaaga
     1081 gaacagatag ttaccgtact cagcggtatc agagataacc acgttcattt cgtacagacg
     1141 cttacgggcg atggtgttgg caatcagcgg cagctcgtgc agtgattcat aatatgcaga
     1201 ctcttcaatg atgccggaat cgaccatggt ttcaaacgcc agctcaacgc ccgctttcac
     1261 catcgcgatc atcagtacgc ctttatcgaa gtactcctgc tcgccgattt tgccttcata
     1321 ctgcggcgcg gtttcgaagg cggttttgcc ggtctcttca cgccaggtca gcagtttctt
     1381 atcatcgttg gcccagtccg ccatcatacc ggaggagaat tcgccggaga tgatgtcgtc
     1441 catatgtttc tggaacaggg gtgccatgat ctctttcagc tgttcagaca gcgcgtaagc
     1501 acgcagtttc gccgggttag agagacggtc catcatcagg gtgatgccgc cctgcttcag
     1561 cgcttcggtg atggtttccc aaccgaactg aattagtttt tctgcgtatg ccggatcggt
     1621 gccctcttcc accagcttgt cgaagcacag cagcgagcca gcctgcaaca taccgcacag
     1681 gatggtttgc tcacccatca ggtcagattt cacttccgca acgaaggacg attccagcac
     1741 gcccgcacgg tgaccaccgg ttgctgccgc ccatgcttta gcgatcgcca tgccttcgcc
     1801 tttcggatcg ttttccgggt gaacggcaat cagcgtcggt acgccgaatc cacgtttgta
     1861 ctcttcacgc acttcggtgc cagggcattt cggcgcaacc atgactacgg tgatgtcttt
     1921 acggatctgc tcgcccactt cgacgatgtt gaaaccgtgc gagtaaccca gcgccgcgcc
     1981 gtctttcatc agtggctgta cggtgcgcac tacgtcggag tgctgcttgt ctggcgtcag
     2041 gttaaccacc aaatccgcct gcgggatcag ttcttcgtaa gtgcccactt taaaaccgtt
     2101 ttcggtcgct ttacgccagg atgcgcgctt ctcggcaatc gcttctttac gcagagcgta
     2161 ggagatatcg agaccagaat cacgcatgtt caggccctgg ttcagcccct gtgcgccaca
     2221 gccgacgata accacttttt taccctgaag gtagctcgcg ccgtcggcga attcgtcgcg
     2281 ccccataaag cgacatttgc ccagctgtgc cagctgctgg cgcaggttca gtgtattgaa
     2341 gtagttagcc atggtgattc ctcgtgatgt tgtgcttctt attgttcggt tcgcgtttgc
     2401 gagattgaat tcactatatg acaggaaatt tattgcggaa attgatatat tcacaacgtc
     2461 acattgcaat ttctgcaacg tcaacattga gggctgctcc attggattta cgcgatctga
     2521 aaaccttcct gcatctggcg gaaagccgcc attttggccg cagcgcgcgg gcgatgcacg
     2581 ttagcccatc cacgctctca cggcagattc agcgcctgga agaagatctt ggtcagccgc
     2641 tatttgtgcg cgataaccgc acggtgacgc tgactgaagc gggcgaagag ctgcgcgttt
     2701 tcgcccagca aacgctgttg cagtatcagc agttgcgcca caccatcgat cagcaagggc
     2761 cgtcgctctc tggcgaatta catatcttct gctcggtgac cgctgcctac agccatctgc
     2821 caccgattct cgaccgcttc cgcgcggaac acccgtcggt ggagatcaaa ctaaccactg
     2881 gtgatgcggc agatgcgatg gaaaaggtag tcactggcga agcggatctg gcgattgccg
     2941 ggaaaccgga gactctgccc ggcgcagtgg cgttttcgat gctggagaat ctggcagtag
     3001 tgctgattgc gcccgcgctg ccctgcccgg tgcgtaacca ggtttcgtca gagaagccgg
     3061 actggtctac agtgccgttt atcatggccg atcagggacc ggtacgccgc cgcattgaac
     3121 tgtggtttcg acgcaataaa atcagcaacc cgatgattta cgccacggtt ggcgggcatg
     3181 aagcgatggt atcgatggtg gcgctcggct gtggcgtggc gctgttgccg gaagtagtgc
     3241 tggaaaacag ccccgaaccg gtgcgtaacc gcgtgatgat tttagagcgc agcgatgaaa
     3301 aaacgccgtt tgaactgggc gtttgtgcgc agaaaaagcg gctgcatgag ccgctgattg
     3361 aggcgttctg gaagattttg ccgaaccatt aacccgccaa aaagaacctg aacgccggat
     3421 tattggtttc gtcgtggcaa tcgtagccca gctcatttag ccgggtttcg aaatccggtt
     3481 catggtcgcc gagttcgaac gccgccagta cgcgcccgta gtcggtgcca tggctgcgat
     3541 agtggaacaa agaaatgttc cagtgcgtac ccagcgtgtt gaggaagcgc agcagcgcgc
     3601 ccggtgattc cgggaattcg aagctgtaga ggcgttcctg caacggatgt gacggacgcc
     3661 cgccgaccat atagcgcaca tgcaacttcg ccatttcgtc gtcggagaga tccaccacgc
     3721 tgtagccgcc gtcgttgagc atctgcaaaa tttctttgcg ctcttcgagg ccgcggctca
     3781 ggcgcacgcc gacaaagatg caggcgtttt tggcatcggc aaaacggtag ttgaactcgg
     3841 tgaccgctcg cccgccaagc agttggcaga atttgaggaa gctgcctttt tcttccggaa
     3901 tggtcaccgc caacaacgct tcacgctgtt cgcccagttc gcagcgttcc gagacgtagc
     3961 gcaggccgtg gaagttcacg ttggcaccgg aaagaatatg cgccagccgt tcgccgcgaa
     4021 tgttgtgctg ggcgatatat tttttcattc ccgccagcgc cagcgcgcca gagggttccg
     4081 ccaccgcgcg cacatcttcg aataaatcct tcatcgccgc acagatcgca tcactatcga
     4141 cggtgatgat gtcgtcgaga tactcctggc ataaacggaa ggtttcgtca ccgatgcgtt
     4201 ttaccgctac gccttcagca aatagcccta cgcgcggcag atcaaccgga tgacccgcat
     4261 ccagcgctgc tttcaggcag gcggagtctt ccgcttctac ggcgatcact ttgatttgtg
     4321 gcatcagttg tttgatcagc accgcaacgc ccgccgccag accgccgccg ccgactggca
     4381 caaatacgcg gtcgagatgg gcgtcctgct ggagcagttc cagcgccagc gtgccttgcc
     4441 cggcaatcac catcggatgg tcgaacggcg gcacccaggt gaacccctgc tgctgtgaca
     4501 gttcgatcgc tttagctttc gcttcgtcaa agttcgcgcc atgaagcagc acttcgccgc
     4561 cgaatccacg taccgcatcg actttgatgt cggcggtggc ggttggcata acgatcaggg
     4621 ccttcacgcc taaccgcgca gaagaaaacg cgacgccctg cgcgtggtta cccgcagaag
     4681 cagtgatcac gccgtgcgct ttttgttctt ccgtcaggcc cgccatcatg gcgtatgcgc
     4741 cgcgcagctt aaagctgtgc actggctggc gatcttcgcg cttcaccaga atgacgttat
     4801 caagacgcga cgacagtttt tccatttttt gtagcggcgt aacctgcgct gcctcgtaaa
     4861 ccggcgcgcg cagcactgct cttaaatatt cggcaccttc cggagcacca gacaggggtt
     4921 gcgagtcagc cattattaac cccccagttt cgatttatcg cgcaccgcac ctttgtcggc
     4981 gctggttgcc aggctggcgt aggcgcgcag ggcaaaggaa acctgacgtt cacggttttt
     5041 cggcgtccag gctttgtcac cccgagcttc ctgcgcttca cgacgcgccg ccagttcggc
     5101 atcgcttacc tgtaactgaa tgccacggtt cgggatgtcg atagcgatca ggtcaccatc
     5161 ttcaatcaga ccaatgctgc cgccgcttgc cgcttccggt gagacgtggc cgatggaaag
     5221 accagaggtg ccgccagaga aacgaccgtc ggtgatcagc gcacaggctt taccgagacc
     5281 cattgatttc aggaagctgg ttgggtagag catttcctgc atgccagggc cgcctttcgg
     5341 gccttcatag cggatcacca ccacatcgcc cgcgacaact ttgccaccga ggatcgcttc
     5401 taccgcgtcg tcctggcttt cgtacacttt tgccgggccg gtgaatttga ggatgctgtc
     5461 atcgacgccc gcggttttca cgatgcagcc gttttcagca aaattaccgt agagcaccgc
     5521 caggccgccg tctttgctgt aggcgtgttc cagcgagcgg atacagccgt ttgcgcgatc
     5581 gtcgtccagc gtatcccaac ggcaatcttg cgagaatgcc tgggtggtac ggatacccgc
     5641 agggccggcg cggaacatat tttttaccgc gtcatcctgg gtcagcataa cgtcgtattg
     5701 ctccagcgtt tgcggcaacg tcaggccgag cacgtttttc acatcacggt tcagtaaccc
     5761 cgcgcgatcc agttcgccga gaataccgat aacaccacca gcacggtgaa catcttccat
     5821 atggtatttc tgggtgctcg gcgcaacttt acacagctgt ggaaccttgc gggaaagctt
     5881 atcgatatca ctcatggtga agtcgatttc cgcttcctgc gccgccgcca gcaggtgaag
     5941 tacggtgtta gtcgatccac ccatcgcgat atccagcgtc atggcgtttt caaacgccgc
     6001 cttactggcg atattacgcg gcagtgcact ttcgtcgttt tgctcgtaat aacgtttggt
     6061 caattcaaca atgcgtttac cagcattaag gaacagctgc ttacggtccg cgtgggttgc
     6121 cagcagcgag ccgttgcctg gctgcgacaa acccagcgct tcggtcaggc agttcattga
     6181 gttagcggta aacatcccgg agcaggaacc gcaggttggg caggcggaac gttcaacctg
     6241 atcgctctgg gagtcagaaa ctttcgggtc tgcgccctgg atcatcgcat caaccagatc
     6301 gagtttgatt atctgatcgg acagtttggt tttcccggcc tccatcgggc cgccggaaac
     6361 aaagatcacc ggaatattca ggcgcaggga agccatcagc atccccgggg tgattttgtc
     6421 gcagttagag atgcagacca tggcgtcggc gcagtgggcg ttgaccatat actcaacgga
     6481 atcagcgatc agttcgcgag atggaagtga ataaagcatc cccccgtggc ccatggcaat
     6541 cccatcatcc accgcaatgg tgttgaactc tttggcaacg ccgccagccg cttcaatttg
     6601 ttcggcgacc agtttaccga gatcgcgcag atggacgtga cccggtacaa attgggtgaa
     6661 cgagttcaca accgcgataa tcggcttgcc aaaatcggcg tcggtcattc cggtggcgcg
     6721 ccacagcgca cgagcacccg ccatattacg accatgagtg gtggtggcgg aacggtactt
     6781 aggcatactt tatttactcc cagtgtctgt ctcgtaaatg ggacggtgcg tgccgtccca
     6841 ttttttgtat ttattgatta acttgatcta accagcccca tttatcttcg gtttcgccag
     6901 tgaagaggcc gaagaaggct tgctgaatgc gtttggtaac cgggccacaa cggccttcgc
     6961 caacctgaat accgtctacg ctgcgcactg gcgtgatttc tgccgcagta ccggacataa
     7021 acacttcatc cgccaggtac agggattcgc gcgacagcac ctgctcacgc acttcaattc
     7081 ccagctcttt cgccagtttg atgatggcat cacgggtaat acccggcagc gcggatgagg
     7141 taaacggtgg ggtgaacagc acgccgtctt tcacttcaaa caggttttca cctgcgcctt
     7201 cagagatgta accgttcaca tccagcgcga taccttcctg ataaccgtgg cggcgcgctt
     7261 cgctacccac cagcagggaa gagaggtagt taccaccggc ttttgccgcc gtcgggatgg
     7321 tgtttggtgc tgcgcggttc caggaggaaa ccatcgcatc gatcccctgc tccagcgctt
     7381 ctgcgcccag atacgctccc cacgggaaag cagcgataat cacgtcggtt gagtatcccg
     7441 ctggcgggtt tactcccatg ccaacatcac cgacgaagat cagcggacgg atataggcgc
     7501 tggtgagatt atttttgcgg atcacgtcac gacaagcttc catcagctca tcaatgctct
     7561 gcgaaaccgg gaagcgatag attttggcgg agtcatgcag acgctgcata tgctcacgat
     7621 ggcggaatac aaccggtcct ttgtgcgagt cgtagcaacg gatgccttca aaaaccgaag
     7681 tgccatagtg cagcgcgtgc gacatcacat gcaccttcgc gtcttcccag cgaaccatct
     7741 ccccattgaa ccaaatgtaa tcagctttct tcgtggtcat ttttatattc cttttgcgct
     7801 caggcgcgga tttgttgtga tgtggttgtg ctctggcaga tggcaacgtg tgcgacgtcc
     7861 accagtttat ttaactgact aaacagtaag tcgaccgacc gtgggctggc aacggtcaat
     7921 tcgatattta tattttgtgc atcgctggcg gcggccatat tcattgagca gacgtggaaa
     7981 ccacgatgac gcaccacgcg taaaacacgt tctaaggttt ccggattgaa gcgagccgat
     8041 acattgacct gatattgcat catgataatt tctccaacat ttctgaatta ctggcgccag
     8101 gcggcaccag cggccagacg ttctcaagtt cgtcgattga gacatgaagc aggtatggcc
     8161 catcactgtt cagcatggtg tcgagtgccg cttcaacctg gtctttacgg gtgatgtgtt
     8221 ggccagggat gccgaaggcg ctggctaaca tgaggaaatc ggggttatca gtaagggtgg
     8281 tttcgctgta tcgttcctga aaaaacagtt gctgccattg tcgaaccatc cctaaccgtt
     8341 ggttatcgag taagacgatt ttcaacggta actgcttgcg ttttacggtg cccagctctt
     8401 gcacattcat catgaaagag ccgtcaccgg agatacagac gacggtatcg ttcggtcgcg
     8461 cgacttgtgc gccaaccgcc gctggtaaac caaaacccat agtgcctaag ccgctggagg
     8521 tgatgaaatt ttccgggcga gtgtgggcga tgtgttgcgc agcccacatc tggtgctgcc
     8581 ccacatctgt ggtcacgacg caatccgcag gtttacgatc cgacagttgt tttaacaaca
     8641 acggcgcgta gatagcgtcg ccgggatggt cgtaacgcca ggcatgttca tcacgcagct
     8701 gcgcgcagta ttgctgccag tcattgatat ttaacggctg ctgtaattct ggtaacagag
     8761 catttaaatc accttgtaat gccacatgtg cctgacgcag cttgttcatt tctgccgggt
     8821 cgatatccat atggataacg ctggcgtgtg gtgcgaaggt gttcagtttg ccggtcaccc
     8881 ggtcatcaaa acgtgcaccc acggcgatca gcaagtcgca ctcctgcacc gcgaagttcg
     8941 ccgctttggt gccatgcatt cccagcatgc ccagatagta cggataatct gcttcaactg
     9001 cgcccagccc tttcagcgtg caggtggcag gcatttttgt ggtagcgaga aattctcgta
     9061 aagcaggaac tgcctgcgcc atacccacgc caccaccaac gtacagcatc ggtttttgcg
     9121 cttttgccag catctggcgc gcttgctcaa cttcggcatg tgggaaagtc acttcgtttt
     9181 caacggtggt gaaccacggt tccaggtcac cgctggctag ctggatatct tttgggatat
     9241 cgaccagaac cggaccagga cgacctgagc tggcaacgtc gaatgcttca gccataatgc
     9301 gcggcaactc ttccagcgac tgcaccagaa agctgtgctt ggtacaggct aacgacaatc
     9361 ccaggacatc cacttcctga aatgcatcag tgccgataaa cggtgcggac acttgaccgg
     9421 tgatggcaac aacagggata gaatctaaca gtgcgtccgc aagcccggtt atcaggttgg
     9481 ttgcgcccgg accagacgtg gcgatacata cgccagtttt gccggtagca cgggcataac
     9541 cgatagccgc cattgccgca ccctgctcat gtcggcacag caagtgctcc acgccgccgt
     9601 catacaatgc atcgtaaacc ggcataattg cgccacccgg ataaccgaaa acggtgttca
     9661 caccctgtgc ccgcaacgca tgtaccaccc actgtgcgcc attcatagtt agttccccgt
     9721 gctgaatgtt gagaaacaga attttgtgct gttattcatt gtctgctcct cggttatgtt
     9781 tttaaggtca aaaaaaaccc ccggaccttt cggtgcgggg gtcttagttc gttaaggctt
     9841 gatttctaag cctttcctcg tccaagtgca gccccgcacg gtgggataat aatcaccacc
     9901 acgctaatca cgaccaggct aatcactcgt agaagggctg tcattttgtc ttttcttgca
     9961 tcttgttcga aggaatgcct aaagagttac cataggtttt gtaaatagta caagatattt
    10021 tttggccgat ttttatgatg cgtttaataa tttcaatata agttgttgtt ttttaaaaat
    10081 aaaatagtga gtaaaaaaat tcaatttcag tatgaatgtt gttcccttcc tcgtagttca
    10141 tcctttgtcc gctgcatttt tgcgagcatc attccaccgc cagaacaact caacttcact
    10201 tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta cagcagcgat
    10261 atcttatgca cataatggtg actcaaggag ggcttatgtc actgtcaatt gttcataccc
    10321 gcgcagccct gggagtaaat gcgcccccga tcactgttga ggtacatatc agtaaaggtc
    10381 tacccggctt aacgaaggtg ggcttaccag aaacaacggt aaaagaagct cgcgatcgcg
    10441 tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc accatcaacc
    10501 tggctccagc tgatctgcca aaagaagggg gacgatatga tttacctatc gccattgcgt
    10561 tgctggcggc ctcagaacag cttacagcca ataagttaga tgaatatgga ttagtcggag
    10621 aactggcgct tacaggcgct ctgcgtggcg ttcccggcgc aatctccagt acaactgaag
    10681 ctattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaagatgaa gtggggctaa
    10741 ttaacggtga aggatgcctg atagccgatc atctgcaagc tgtctgtgcg tttctggaag
    10801 gtaagcacgc tctcgaacgc ccgaaaccaa ctgatacagt atcccgggcg ctacaacatg
    10861 atctcagtga tgttatcggt caggaacaag gaaagcgagg actggaaatt accgccgctg
    10921 gcgggcacaa ccttttactg attgggccgc cgggaacagg taaaacaatg ctcgccagcc
    10981 gtattaatgg cctgttgcca gatttaagca atgaagaggc actggagagt gctgcgatat
    11041 taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac
    11101 ctcatcacag tgcatcgtta actgcgatgg taggcggtgg cgcaattcca gggcccggtg
    11161 aaatttcgct ggcgcataac ggcgtgcttt ttcttgatga gttacctgaa tttgaaaggc
    11221 gtacactgga tgccttgcga gagccgattg aatccgggca gatccatctt tcacgcacgc
    11281 gagcaaaaat aacctatcca gcccgtttcc agcttgtcgc ggcgatgaat cccagcccta
    11341 ccggacatta tcagggaaac cataaccgct gcacaccaga gcaaacgctg cgttatctca
    11401 accgactctc tggtcccttt ctcgaccgct tcgatctctc actggagatc ccattaccgc
    11461 ctcccggtat tttgagtaaa acggtagtgc cgggagaaag cagcgccacc gttaaacaac
    11521 gcgtaatggc cgccagagag cgccaattta agcggcagaa taaactgaac gcctggctgg
    11581 atagtccgga aatacgccaa ttctgcaaac ttgagagcga agatgcgatg tggctggaag
    11641 aaacgctgat ccatctgggg ttatcgattc gtgcctagca gcggttattg aaagttgcac
    11701 gaaccattgc tgatattgat cagtctgaca ttatcacacg tcaacatttg caggaggcag
    11761 ttagctatcg agcgattgac cgtttgctca tccatctgca gaaactactg acataaaaaa
    11821 agggcatttc gcccttttta ttaatcgtca gaatcggtgt agtcttcagc accttcaacc
    11881 tgcggtttac cgccggaaag ggtgtgaaaa cgttttggac gcttaatacg cgtcatatac
    11941 ttggaccaca cgcgttctgc ttctgtcact ggctcacgtt cgccacggca tactgctacg
    12001 aagagtttct cttcctcggt aaccggctcg cgtttgccaa gatccaactc attgaaggca
    12061 taaccatgac gctcaagcag ttgtgcctct ttgatggtga aatcaccatg acgagagaat
    12121 ccacgtggat aatgtttatt gtcgaaatat cgattagtcg tcgtaaagct ttccgccatc
    12181 ctgcacgctc ctaattcttt gaccgagcta gttatggcgc ggagtattag ttacgcttga
    12241 cagagtgtaa aacaaaacat ttaaatcata acgacaaata attttgcgga gagcactgtg
    12301 gatacggaat tgttaaaaac tttcctggaa gttagccgaa cgcgtcactt tggtcgagcg
    12361 gctgaatcgc tctatctgac ccagtcagca gtgagctttc gaatcagaca actggaaaat
    12421 caactgggtg tgaacctttt cacccgccac agaaacaata tccgtttaac cgctgccggt
    12481 gaaaaactac tgccttatgc agaaacgctc atgagcacct ggcaggccgc ccgcaaggag
    12541 gtggcgcata cctcacgaca taacgagttt tctatcggtg ccagcgcctc attgtgggaa
    12601 tgtatgctta atcagtggct gggacgtttg tatcaaaatc aggatgccca tacaggctta
    12661 cagttcgaag cgcgaattgc ccaacggcag tctctggtaa aacagctgca tgaacgccag
    12721 cttgatcttc ttattaccac tgaagcgccc aaaatggacg aatttagtag tcagttgctg
    12781 ggatatttca ctttagcgct ttataccagt gccccttcaa aactaaaggg agatcttaat
    12841 tatctgcgac ttgagtgggg gccagatttt caacagcatg aggcaggttt gatcggtgct
    12901 gacgaagtgc ccattctgac aaccagttct gctgaactgg cacagcaaca gattgcgatg
    12961 cttaatggtt gcacctggct acccgtcagc tgggcgcgta aaaaaggcgg cctgcatacc
    13021 gttgtcgata gcacaacact ttcacggccg ctttatgcca tatggctgca aaatagcgat
    13081 aaaaatgcgt tgattcgcga tcttttgaaa attaacgtgc tggatgaagt gtattaatat
    13141 gaatggctgg caaggatgcc ggtagaagga tttacttcgg agagggttat ttcagataaa
    13201 aaaaatcctt agctttcgct aaggatgatt tctggcaggg gcggagagac tcgaactccc
    13261 aacacccggt tttggagacc ggtgctctac caattgaact acgcccctaa ttag
//
LOCUS       DAJTOR010000111        13282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTOR010000111 DAJTOR010000000
VERSION     DAJTOR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.111"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(97..1020)
                     /gene="dmlR"
                     /locus_tag="OO249_002818"
     CDS             complement(97..1020)
                     /gene="dmlR"
                     /locus_tag="OO249_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCR5637051.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            1123..2208
                     /gene="dmlA"
                     /locus_tag="OO249_002819"
     CDS             1123..2208
                     /gene="dmlA"
                     /locus_tag="OO249_002819"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCR5637052.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMG
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            2458..4068
                     /gene="yeaV"
                     /locus_tag="OO249_002820"
     CDS             2458..4068
                     /gene="yeaV"
                     /locus_tag="OO249_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCR5637053.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVIPAGTVFKGDN"
     gene            4100..5224
                     /gene="yeaW"
                     /locus_tag="OO249_002821"
     CDS             4100..5224
                     /gene="yeaW"
                     /locus_tag="OO249_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCR5637054.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTSANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            5280..6245
                     /gene="yeaX"
                     /locus_tag="OO249_002822"
     CDS             5280..6245
                     /gene="yeaX"
                     /locus_tag="OO249_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5637055.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(6299..7414)
                     /gene="rnd"
                     /locus_tag="OO249_002823"
     CDS             complement(6299..7414)
                     /gene="rnd"
                     /locus_tag="OO249_002823"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5637056.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7495..9180)
                     /gene="fadD"
                     /locus_tag="OO249_002824"
     CDS             complement(7495..9180)
                     /gene="fadD"
                     /locus_tag="OO249_002824"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5637057.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGVGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAVVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(9385..9966)
                     /gene="yeaY"
                     /locus_tag="OO249_002825"
     CDS             complement(9385..9966)
                     /gene="yeaY"
                     /locus_tag="OO249_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5637058.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGRSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10006..10701)
                     /gene="tsaB"
                     /locus_tag="OO249_002826"
     CDS             complement(10006..10701)
                     /gene="tsaB"
                     /locus_tag="OO249_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5637059.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(10759..12669)
                     /locus_tag="OO249_002827"
     CDS             complement(10759..12669)
                     /locus_tag="OO249_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5637060.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            12801..13145
                     /locus_tag="OO249_002828"
     CDS             12801..13145
                     /locus_tag="OO249_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5637061.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3171 a         3533 c         3324 g         3254 t
ORIGIN      
        1 cgcgattaaa ggtgaatatg ctaaccaatc tgtagcggct tagaaaggag aaaatcaggt
       61 tttaacccga tatcaacccg ataattgaat cattaatcag gcatgctcca gtgaaaaatt
      121 cgggtagtgc tcggcaaaat actggcgtaa aaattctacc gttatccgca ctttcgctga
      181 cgtcgccagc cttgaaacat aaacggccca gacgttcgct ggctggtaat attccggtag
      241 cacttgcact aaatgaccac tggcaatgtt ttcgctaaca tcccaccagg aacgcagagc
      301 aatcccctgc ccatcgagac accactgatg gacaatttct ccatgattag acgacagtgg
      361 gccggtgact ttaatcgcat ggtggccttc tttattgcgt aattgccaga caccaaaagg
      421 atgatcgcgc tctttaatga ccaggcacgg caatgcagaa agatcggtta aatgtttcgg
      481 cgcgccatgt tgcgcaataa attccggcga cgcgcagaga atgcggtaat tggtcgccag
      541 tttgcgggcg attaaattag gggcgatgtc atcgccaatg cgaatatcga gatcgacacc
      601 ttcattgacc aaatcgacca gccggtcttc cacatcaaaa cgtaattcca gttgcggata
      661 cgccttcgcc agcgctgata acgccggagc caccacctgt cgtccaaaac caaagctgct
      721 gataatacgc agcatcccct gtggcacctg acgcacgtca gaaagttcgt ccatcatctg
      781 accgacatcc tgcaaaatcc gctgcgccca ttcataaatc cgctctcctt cttcggtaat
      841 ggtgacgcgg cgggtggtgc ggtgtaacaa caccacgttt agcgtttgct ccagcaaggc
      901 gatgcgcttg ctgacgaacg ccggtgaaac gcccagttct tccgccacgg cggcaaaacc
      961 agcccgacga gccaccagca taaagacgcg caaatcattc agcagcggta aattattcat
     1021 gattcgtgtt ttatgtttca ccagttacgg ggattaattc cttttccgtc aattatagga
     1081 tggtgatgtt gtcaattttg atggtcagga agtgagaacc caatgatgaa aacgatgcgt
     1141 attgctgcga tcccgggaga cgggattggt aaagaagtcc ttcctgaagg gattcgcgtg
     1201 ttacaggctg ccgctgagcg ctggggcttc gacttgagtt ttgagcaaat ggggtgggcg
     1261 agctgcgagt attacagcca tcacggtaaa atgatgccgg acgactggca tgagcaactt
     1321 agccgtttcg acgccatcta ttttggtgcc gtcggctggc cggataccgt tccggaccat
     1381 atttcgttgt ggggttcgct gctgaaattt cgtcgtgaat tcgaccagta catcaacctg
     1441 cgcccggttc gtctctttcc tggcgttccc tgcccgctgg cgggaaaaca gcctggcgac
     1501 atcgattttt acgtggtcag ggaaaacacc gaaggcgaat attcctcgct cggcggtaga
     1561 gtgaatgaag gtacagagca tgaagtcgtc attcaggaat cggtatttac acgtcgtggt
     1621 gtcgatcgca ttttgcgtta tgccttcgaa cttgcgcaaa gccgcccacg taagacgcta
     1681 acttatgcca ctaaatcgaa cggtttagcc atcagcatgc cgtactggga tgagcgagtg
     1741 gaagcaatgg ccgagaatta cccggagatc cgctgggaca agcagcatat tgatattctc
     1801 tgcgcgcgtt ttgtgatgca gccggaacgc ttcgatgtgg tggtggcgtc caatttgttt
     1861 ggcgatatcc tttccgatct tggcccggcc tgcaccggca ccattggcat tgccccatcc
     1921 gccaacctga atccggaacg cactttcccg tcgctcttcg agcctgtcca cggttccgcg
     1981 ccggatatct acgggaaaaa tattgctaac cctatcgcca cgatttgggc cggggcaatg
     2041 atgctcgatt ttctcggcaa tggcgatgag cgtttccagc aagcgcataa cggtattctg
     2101 gcagcaattg aagaagtgat tgctcacggg ccgaaaacac ctgatatgaa aggcaatgcc
     2161 accacgccac aggttgccga cgcgatttgc aaaattattt tgcgttaagg tcaaaccagt
     2221 ttatttgaac cgcgtcactg acgcggtttt ttattcgttc tttgcagtaa ataacctgcg
     2281 tcatttcacc ttttattgtt tccgtttcgt gttttatggc tttccgcatt cttaattgtt
     2341 taatttatgt aatattcaaa taatcaattt attaaacaca acatacaact cattaccctg
     2401 caaataatta caactggcaa aagccagaga cgggctttta tgcccaggag atgactcatg
     2461 atgagcaatg ttaagaaaaa agatgtgccg ctgataagca tcagcctggt ggccattctt
     2521 ttcatcgcag ctgcattaag ccttttccca caacaatcgg ccgacgcggc caacgcaata
     2581 tacacttttg ttacgcgtac gttaggttcc gccgtacagg tattggtttt gctggcaatg
     2641 ggactggtga tttatttagc caccagtaaa tacggcaata ttcgtcttgg cgaaggaaaa
     2701 ccggaataca gcacgctctc ctggctgttt atgtttattt gtgccggttt aggttcttct
     2761 acgctttatt ggggggttgc tgaatgggcc tattattatc agacacctgg attaaatatc
     2821 gcaccgcgtt cacaacaggc actcgaattt agcgttccct actctttctt ccactggggc
     2881 atcagcgcct gggcaactta tacgctggcc tcattaatca tggcttatca ctttcatgtg
     2941 cggaaaaaca aaggtctgag cctttccggc attattgctg ctattaccgg cgttcgcccg
     3001 caaggcccat ggggaaaact ggttgatttg atgttcctga tcgccactgt cggcgcactg
     3061 actatttccc ttgttgtcac cgcagcaacc tttactcgtg gactttccgc actgacaggt
     3121 ttacccgaca acttcaccgt gcaggcattt gtgatcctgc tttctggcgg cattttttgc
     3181 ctaagctcgt ggattggtat caacaacggt ttgcaacgtc tgagcaaaat ggttggctgg
     3241 ggcgcgttcc tgctgccatt actggtgctg attgtcggcc caaccgaatt tattaccaac
     3301 agcatcatca atgccatcgg cctgaccacg caaaacttcc tgcaaatgag cttattcacc
     3361 gatccgcttg gcgatggttc atttacccgc aactggaccg ttttctactg gctgtggtgg
     3421 atctcataca cccctggcgt agcaatgttt gtcacccgcg tttcccgcgg tcgtaagatt
     3481 aaagaagtta tctggggact gatcctcggc agcaccgtcg gttgctggtt cttcttcggc
     3541 gtaatggaaa gctatgccat tcatcagttt atcaatggcg taatcaacgt cccacaggtg
     3601 ctggaaacac tgggcggcga aacagctgta cagcaagttc tgatgtcgtt gccagccggt
     3661 aaattgttcc tcgccgcata cctgggcgtg atgattattt tccttgcctc gcatatggat
     3721 gcagtggcct acaccatggc ggcgaccagt acgcgtaatc tccaggaagg tgacgatcct
     3781 gaccgtgggc tgcgtctttt ctggtgcgtg gtgatcactc tgatcccgct ttccatcttg
     3841 tttaccggtg cttcgctgga aacgatgaaa accaccgtcg tgctcacagc ccttcccttc
     3901 ctcgtcattt tactggtgaa agtcggcgga tttattcgct ggctgaaaca ggattacgcc
     3961 gacattccgg ctcatcaagt tgaacattat ctcccgcaga caccggttga agccctggaa
     4021 aaaacaccag tgatccctgc gggaaccgta ttcaaaggcg acaactgagc gccatcatcc
     4081 taacgataaa ggtatcccta tgagcaatct gagccctgac tttgtactac ccgaaaattt
     4141 ttgcgctaac ccgcaagagg cgtggaccat tcctgcccgt ttttataccg atcagaacgc
     4201 gtttgaacac gaaaaagaga acgtcttcgc caaaagctgg atttgcgtcg ctcacagcag
     4261 cgaactggcg aatgccaatg attatgtgac gcgtgagatc attggcgaaa gcatcgtgct
     4321 ggtacgcggt cgtgataagg ttttgcgcgc gttctataac gtgtgtccgc accgtggtca
     4381 tcagttgttg agcggtgaag gaaaagcaaa aaatgtgatt acctgcccgt atcacgcatg
     4441 ggcattcaaa ctcgatggca atctggccca tgcacgtaac tgcgaaaacg tcgccaattt
     4501 cgatagcgac aaagcgcaac tggttccggt gcgtctggaa gaatatgccg gattcgtctt
     4561 catcaacatg gaccccaacg ccaccagcgt agaagatcag ttacccggtc tgggcgcgaa
     4621 agtgctggaa gcctgcccgg aagtccacga tctgaaactg gcggcccgct ttaccacccg
     4681 cacgtctgcc aactggaaga acattgtcga taactatctc gagtgctatc actgtggtcc
     4741 ggcgcatcca ggtttctccg actccgtaca ggttgatcgt tactggcaca ccatgcacgg
     4801 taactggacg ctgcaatacg gtttcgccaa accgtccgag cagtcgttta aatttgaaga
     4861 gggtacggat gcggcattcc acggtttctg gctgtggccg tgcacgatgc tgaacgtcac
     4921 cccgatcaaa gggatgatga cggtcattta tgaattcccg gtggattctg aaactaccct
     4981 gcaaaactac gatatttact tcaccaatga agagttaacc gacgagcaaa aatcgctgat
     5041 tgagtggtat cgcgatgtgt tccgtccgga agatttacgt ctggttgaaa gcgtacagaa
     5101 agggctgaaa tcgcgtggct atcgtggtca ggggcgcatc atggccgaca gtagcggtag
     5161 tggcatttcc gaacatggta tcgcccattt ccataatctg ctggcgcagg tgtttaagga
     5221 ctaatgacat cggcggcggt attttccgct gctgggctga tttttgatgg agtacagcaa
     5281 tatcagacta tcaaatgttt gaagtacagg tgagccaggt tgaacccctt accgaacagg
     5341 tgaaacgctt cacgctggtg gcaaccgatg gcaaaccgct gcctgcgttc accggaggaa
     5401 gtcacgtcat tgtgcagatg agcgatggtg ataaccagta cagcaatgcg tattcactac
     5461 tgagttcgcc gcatgacacc tcttgttatc agattgccgt tcggctggag gaaaactcgc
     5521 gcggcggttc ccgctttttg catcagcagg taaaagtggg caatcggtta acgatttcaa
     5581 cgcctaataa cctgtttgcg ctaattccct cagccagaaa gcatctgttt atcgcgggcg
     5641 gtattggtat cacccctttc ctgtcgcaca tggcagagct gcaacacagc gacgtcgact
     5701 ggcagctaca ttactgctcg cgaaatccag aaagttgcgc atttcgtgat gagctagtcc
     5761 agcatccgca ggctgagaaa gttcacttgc atcactcatc aaccggaaca cgactggaat
     5821 tagcgcgatt attggcggat atcgaacctg gcacacacgt ttatacctgt ggtcccgagg
     5881 cgctaaatga agcggtaaga agtgaagctg cgcgtctgga catcgccgcc gatacgctgc
     5941 actttgagca atttgctatc gaagacaaaa ccggcgatgc ctttaccctg gtgcttgccc
     6001 gttccggaaa agagtttgtg gtgccggaag agatgactat tttgcaggtt attgaaaata
     6061 ataaagccgc gaaagtggaa tgtttatgtc gtgaaggggt atgcggaacc tgcgaaacag
     6121 caatactgga aggtgaagct gaccatcggg atcagtattt tagcgatgaa gagcgtgcca
     6181 gccagcaaag tatgttgatc tgttgttcgc gtgcgaaggg taaacgcctg gtgttggatt
     6241 tgtagtttgc caaagccgga tgtggcgctg agcgcgccca gtccggcttc ggaagatttt
     6301 actgcggata ttcctgcaat aaattgtgta atgcttccgc catcagctca ccacgccagc
     6361 cggaaatcag ctccggcaaa ttgttctgcg gtttcagttt ccagtgccag ttcagcagtt
     6421 ggttgatttg ccgacgcgat gccagcaatt cggcgctgat cttatgcgtt tcgctcacgt
     6481 cagtaatcag cgacttaatc gctttaaacg ctttacgata accaggcatg tccatcaggt
     6541 taagcatcgg ctgcggtaag gcctcttccg gcaatgtctg cgctttttcc accagcgtca
     6601 gcagcgtttt accgtgaaag cggatttcgc taccggataa acccaggctg tccagttcgc
     6661 ctaaacttcc cggcatataa cgcgcgaccg accacaaatg ctcttcacgc acgacaaagt
     6721 tcaccgccag atcacgctct cgcgccttgc gcagtcgcca gtcggctaac agttgcagac
     6781 aggccagttg gcgtgtgcgt aactgccagg cattggtgat atcacgccag gcatcttccg
     6841 gcgcaacgac ttcctgacga cgcatttgca tcaggcggca ttcatccagc gccgcaggta
     6901 gccagccgga ggcctccgtt tctaccataa gcttggcggt gatcggtaac agataccaga
     6961 catccgccgc tgcgtattca cactgacgtt cggtcagcgg tctggccagc cagtcggtgc
     7021 gcgattcact cttgtccagc gtaacgccgg aatactcttc caccatggaa gcgaaacccc
     7081 atgacatcgg gcgtccgcag aaggcagaaa ggatttgcgt gtcaatcaag ggttgtggta
     7141 attcgccaaa gacattgagg aacacttcca gatcttcact gcctgcatgg agaaattttg
     7201 tgatggacgg atcgcgcagg atcgctttca gcggtgacca gtcggtgatc ccgagaggat
     7261 caattagcgc cagatgctcg ccatcgaaaa gttgaatcaa ccccagctgc gggtaataag
     7321 tgcgcgtacg aacaaattca gtatccaggg ctatcgccgg aaaggcacgg acggcttcac
     7381 acaaagaagc cagcgcatcg tccgtggtaa tcatttggta attcaaattg ttttctcttt
     7441 agtgggcgtc aaaaaaacgc cggattaacc ggcgtctgac gactgactta acgctcaggc
     7501 tttattgtcc actttgccgc gcgcttcgtc acgtaattct cgtcgcaaaa tttttccgac
     7561 gttagatttc ggtaactcat cacgaaactc caccagcttc ggtactttgt atcccgtgag
     7621 ctgacggcgg caaaaagtca ccagtgactc ttcggtaagc gatggatctt ttttcactac
     7681 gaagattttc accgcttcac cactggagcc ggaaggtacg ccaacagccg cgacttcctg
     7741 tacgccagga tgctgcatga cgacatcttc aatctcgttg ggatagacgt taaaaccgga
     7801 aaccagaatc atgtcttttt tacgatcgac aatgcgcagg aatccttctt catccattac
     7861 cgcgatgtcg ccggtgtgta accagccatt tttgatgatt tcatcggtag catcgggacg
     7921 ctgccagtaa cccagcatca cctgcggtcc tttgacacaa agctcacccg gttgacctgg
     7981 tggtacttca ttatcatcat catccaccag tttggcttcc gtcgacggca ccggcaaacc
     8041 gatgctacca ctatgataat caatatcata tgggttaacg ctgaccagcg gcgcacactc
     8101 ggtaaggcca tagccttcca gcagatactg tccggtcagt ttcacccaac gctctgccac
     8161 cacttgctgc actggcatcc caccgcctgc ggaaagatgc agactggaga aatccagctg
     8221 ctggaactct ttattgttca gcaacgcatt gaacaaggtg ttaacgccag tgatagcggt
     8281 aaacggatat ttcgctaact cttttaccaa ccctggaata tcacgcgggt tagtgataag
     8341 caggttctgc ccacccagtt cgataaacag caggcagtta atggtcaggg caaaaatgtg
     8401 atacagcggc agtgccgtta ctaccagttc tttacctgga tgcagcaacg gcccataggt
     8461 ggcgttgacc tgttccaggt tcgccagcat attgcggtga gtcagcatcg cgcctttcgc
     8521 cacacctgtg gtgccgccgg tgtattgcag gaaggctaaa tcttccggca ccagttcggg
     8581 tttgacgtac tgcatccggt agccgttatg cagtgcgcta cgaaatgaaa tggcatctgg
     8641 cagatggtat ttcggcacca aacgcttgat gtatttaaca acgaaattga ctaccgtgcc
     8701 ttttgccgta gatagctgat cgcccatacg ggtcagaatt acgtgctgaa cggcggtttt
     8761 atcaaccact ttttccagtg tgtgagcaaa gttagacacg ataacaaccg ccgatgcgcc
     8821 gctatcgtta agctgatgct caagctcacg cggggtatac aacgggttaa cgtttacgac
     8881 gatcatcccg gcacgcaaaa tgccaaacag cgccaccgga tattgcaata aattaggcat
     8941 catcaacgca acgcgatcgc ctttcttcag ccccacccct tgttgcaaat aagcggcaaa
     9001 cgcgcgactg cgttcttcca gcttgcggaa ggtcatcacc tcccccatat tcacaaacgc
     9061 aggctgatcg gcgtagcgcg cgaccgactg ctcaaacata tctaccagag attgataacg
     9121 gtcagggttg atctccgtcg gaacgtccgc gggataacgg ttaagccaaa ccttcttcaa
     9181 ttcttcacct ctaaaatgcg tgttcgtcgt catcgcaacc ccaaatgata tacatgccgt
     9241 taacataata ttaactcatc ataccagctt gataattacc caacgaaaag gttgcgaagc
     9301 gcgtcactat ttatttttat ctttaccgta agaatgcaga aacagcggac cagccgctgt
     9361 ttctttttct ttaaaaacaa gcaattattc agttacaact gtttgtactc tcgcgggacc
     9421 gggattatac cagccccatc cgccgtagcc atagggccag ccacgaccgc cataaaacca
     9481 tggatcaatc ggctgaggcg gcataatcac ctgctgggtt aaatgccaac gtttgtaacc
     9541 cgttacttgc atcaccataa atttataggg cgtattgccg attttgccgt caaccgcacc
     9601 agtgattggc ccgactaccg taaccagttg tccacggaaa tccaccgggt ccagaaaacc
     9661 gttcacatcg gcataaatgc gaccgcgaga aggttctccc agcgtcggtc tggctccgct
     9721 gtccagcggt acggtagcaa tttccaggcg ggttttccct tgctggtttt gtaccgcaac
     9781 cactttgcca ccaaagcgtg cctcctgacc aacgtacagc tgcggcgcac tcatcacccg
     9841 aactaaatct tgttgcggcg tgggactgcg gcctttaatg gcgtccggca cagtgacaca
     9901 accgctcagc attagcgcaa acgtacctgc cagtatgcct ttgataacat tcttttgaac
     9961 cgccatgatg cgactccttt ttttctcaga gcatactctt aagattcatt ctttgcccgg
    10021 aagtttcttc catgcgacgt tgttacgtaa ataaaccggt tcggcatgtt ccaccgccac
    10081 cgttttaccc tcggcaaata tctgacacgc aatcggcagc atatcttccg tcgctggcag
    10141 taaaacttct ccgtcgcgca aaaccagccc gctctcttta ccgagatccg gccaggcttg
    10201 ccagcccgtc cctaccgtta cccattcgcc ggaaagctgt tgcattcgtt catgaacgag
    10261 ttcgggtttg agtacggctt cggtttcttc accgcgccag ataccgtttt catcacgctg
    10321 atattcggcc cagtaaactt cgcccatccg cgcatcaatg gcagccagca cgcgggttgc
    10381 gccgtttttg cgccacgccc cttgcgccat tgtcattagc gtggagacgc caatcatcgg
    10441 cagctctgcg ccaagtgcca gcccttgcgc aatgccaata ccaatgcgca cgccggtaaa
    10501 gctaccgggg ccgcgcccgt aagccagagc gttaatatca gtcagggaag ttccgctggt
    10561 ggtcaggata tcctgcacca tcggtaagat tcgttgagta tgttcacgag ggcaaagctc
    10621 aaaatgagcg ttgacagtac cgtcgttcca cagggcgaca gagcaggcct ctgtcgcggt
    10681 atcgatagcc agaattcgca tgggtcttcg tgcttagatc aataaaaagg cgcgcatcat
    10741 accatactcc gtaacaaatt acctggagga tggtatcgca aggaaacgaa ccgcacgggc
    10801 aatgtcacgg gtgcgcggcg cgggcggcag actggcgaga aacgtcgcgc cgtaagggcg
    10861 catcaccagc cgattgtcac aaatcaccaa aaccccgcga tcgtcggcgt cgcgaatcag
    10921 tcgccctacc ccctgcttga gagtaatcac cgcatccggc agttgtactt catcgaacgg
    10981 gtcgccaccg cgcaaacggc aatcttccat gcgcgctttt aacagcgggt catccggtga
    11041 ggtaaacggc aacttgtcga taatcaccaa tgacaatgta tcgccacgca cgtccacccc
    11101 ttcccagaaa ctactggttg ccacaagaag cgcgttaccg gcgctgacaa attgctgcaa
    11161 cagttgccct ttgctggttt ccccctgcaa caaaacggga agcgtcatgg tagcgcggaa
    11221 ctgctcggcc agatcacgca tcatggcgtg cgaggtacaa agcataaaac aacgaccgtt
    11281 gttagcttcg atgatcggtc gcagcattgc cgctaactgg cgagcagaac ctggctggtt
    11341 ggtttgcggc agattgcgcg gcacacagag taacgcctgg cggctgtaat caaacgggct
    11401 tggcaatagc aacgactccg cctgttctat cccaagccgc gaggtgaaat gatgcagatc
    11461 gtcgttcacc gacagcgttg ctgaggtgaa gatccagcta ccaggctttt gcgccattaa
    11521 ctctttgaat ttatccgcca cgctgagcgg cgtgagagcc agagtaaaat ggcgcgaagt
    11581 gcattcgtac cagtagctgt agcccggctg attgatctct tttagccgct tcagccgtgt
    11641 gcgatacaac gtagcgcgct caaatgccgc atccagcaag gcggaacgcc ccagtgacag
    11701 tttcgccacg tcataacaaa gttccagggt gtcatcgagc agtaaaaatg cccgctgaat
    11761 ttgcggatta gctaacagct cacgcaggtt accgcgataa cctggctcac cgagttgcag
    11821 acgaaaatcc tgcgcactct gggcaagacg atcagcgcac ttttgtaact gctgggtgtc
    11881 ttttaattcg gtgcggtagg cgatggtgat gtcttttgcc aggtcgagca gttgtcgact
    11941 ggagagtgac tgaccaaaat actggctggc aatgtccggt agctggtggg cttcgtcgaa
    12001 gatcatgacg tccgcttccg ggatcagttc gccaaatcca ctctctttaa ccaccatatc
    12061 cgccagaaag agatgatggt ttaccaccac cacatcggcg tccatcgctt ttttacgtgc
    12121 tttgaccaca aagcaatctt tatacatcgg gcagtcgctg ccaagacagt tgtcgttggt
    12181 gctggtaacc agcggccacg cctgtgaatc ttccgccacg ctgacgcagg tgctgatatc
    12241 accatcgact gtttgattag accaggagcg cagcaggatc acatcgctta agatttgtac
    12301 cggcagatcg ccccccgcca gcgcctgctg ttcgagacgt tcgaggcaga ggtagtttga
    12361 gcgccctttc agcagcgcca cgttgcccgt atatttcaat gcctttgaga ctgttggcaa
    12421 atcgcggctg tagagctgat cctgcaacgc ttttgagccg gtcgagataa tgactttctt
    12481 tttcgcccgc agcgcaggag ccaggtaagc gtaggttttg cccgtaccgg ttcctgcttc
    12541 caccaccagc ggctggcctt tttctatcgc ctgggtgacg gctaccgcca tctgtcgctg
    12601 tggttctcgc ggcttaaagc ctggtatcgc tttcgccagc tgaccgtctg gtgcaaaatc
    12661 gtccgtcaca ctaccccctg ttgatttgaa cggggattat gtcaggatga gggcgctttc
    12721 gccagttgaa gtggtgacgg caacctcaca ttgtggcagt ctttgcagca cgaaaatgga
    12781 agtataatga ggacaaaatg atgactatcg ttcgtatcga tgctgaagcc cgctggtctg
    12841 atgtagtaat ccacaacaac acgctctact acactggtgt accggaaaac ctcgacgccg
    12901 atgcttttga gcaaaccgcc aacacgctgg cgcagattga cgccgtgctg gaaaaacagg
    12961 gcagcaataa atcgagcatt ctggatgcca ccattttcct ggctgataaa aacgacttcg
    13021 cggcgatgaa taaagcgtgg gatgcctggg ttgtggcggg tcatgcgccg gtgcgctgca
    13081 cggtgcaagc ggggctgatg aacccgaagt ataaagttga aattaagatt gtggctgcgg
    13141 tgtaagcttt atcgaagcaa aataagtcag acgataattt atcgataata ctggtcggtt
    13201 ttacataaat cgaccagaga ttaagatatt actgttgatg ctcctgctaa ttcattattt
    13261 atttttataa tttcaatttt ag
//
LOCUS       DAJTOR010000112        13189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTOR010000112 DAJTOR010000000
VERSION     DAJTOR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.112"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..182)
                     /locus_tag="OO249_002829"
     CDS             complement(<1..182)
                     /locus_tag="OO249_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR5637062.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWL"
     gene            complement(290..530)
                     /locus_tag="OO249_002830"
                     /pseudo
     CDS             complement(290..530)
                     /locus_tag="OO249_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(757..1578)
                     /locus_tag="OO249_002831"
     CDS             complement(757..1578)
                     /locus_tag="OO249_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5637063.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            complement(1575..1955)
                     /locus_tag="OO249_002832"
     CDS             complement(1575..1955)
                     /locus_tag="OO249_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5637064.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(1956..2939)
                     /locus_tag="OO249_002833"
     CDS             complement(1956..2939)
                     /locus_tag="OO249_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5637065.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLAEDKSLLPFFS
                     DERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLGQLAHQNLFAWMIDVIRHAMNGSQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQNPPQEKTVVSI
                     AVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKA
                     DDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            complement(3013..3285)
                     /locus_tag="OO249_002834"
     CDS             complement(3013..3285)
                     /locus_tag="OO249_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637066.1"
                     /translation="MAHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(3453..3608)
                     /gene="hokD"
                     /locus_tag="OO249_002835"
     CDS             complement(3453..3608)
                     /gene="hokD"
                     /locus_tag="OO249_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5637067.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(3846..4511)
                     /locus_tag="OO249_002836"
     CDS             complement(3846..4511)
                     /locus_tag="OO249_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5637068.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(4686..5111)
                     /locus_tag="OO249_002837"
     CDS             complement(4686..5111)
                     /locus_tag="OO249_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5637069.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRLSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            complement(5127..5897)
                     /locus_tag="OO249_002838"
     CDS             complement(5127..5897)
                     /locus_tag="OO249_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR5637070.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRKGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(5919..6661)
                     /locus_tag="OO249_002839"
                     /pseudo
     CDS             complement(5919..6661)
                     /locus_tag="OO249_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(6668..7630)
                     /locus_tag="OO249_002840"
     CDS             complement(6668..7630)
                     /locus_tag="OO249_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5637071.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASYDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(7653..8078)
                     /locus_tag="OO249_002841"
     CDS             complement(7653..8078)
                     /locus_tag="OO249_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5637072.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDPPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(8062..8385)
                     /locus_tag="OO249_002842"
     CDS             complement(8062..8385)
                     /locus_tag="OO249_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5637073.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            8510..8986
                     /locus_tag="OO249_002843"
     CDS             8510..8986
                     /locus_tag="OO249_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637074.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKKAIVAFILSGNATEPSWVDYDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            9305..9457
                     /locus_tag="OO249_002844"
     CDS             9305..9457
                     /locus_tag="OO249_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5637075.1"
                     /translation="MDTIELGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LTRNTD"
     gene            9469..9789
                     /locus_tag="OO249_002845"
     CDS             9469..9789
                     /locus_tag="OO249_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637076.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLDLMDIIDKGFGLLMKKKGIRIEPLENYFATKSINS
                     FDSK"
     gene            complement(9767..10042)
                     /locus_tag="OO249_002846"
     CDS             complement(9767..10042)
                     /locus_tag="OO249_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637077.1"
                     /translation="MSTENDEIINSLIRQINNFDKALQHAAARSDITLLAISFLASVM
                     DKNEVVRQSLVDYIDSLQPGTFNHESFNHEKEHVKSVINSLILNQKN"
     gene            10604..10792
                     /locus_tag="OO249_002847"
     CDS             10604..10792
                     /locus_tag="OO249_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCR5637078.1"
                     /translation="METLLPNINTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            10789..10980
                     /locus_tag="OO249_002848"
     CDS             10789..10980
                     /locus_tag="OO249_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCR5637079.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            11073..>13189
                     /locus_tag="OO249_002849"
     CDS             11073..>13189
                     /locus_tag="OO249_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5637080.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RTNQQHSENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSV
                     NQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQSGGGNCPDCG
                     AVMGDATYQETFDEENQVEVQENDPKEMEGAEHPHKENAGSAQDHASDSETGETADPL
                     IAMNGHHVITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDVIKRWLKQSREA"
BASE COUNT         3390 a         3454 c         3031 g         3314 t
ORIGIN      
        1 tccagccact tcaggtaatc atcgtcgccc ttccactggt tatcccagcc ctcaggcttc
       61 actttaaagt acggcgggtc cgtgactatc aggtcaacag aattttcggg taacgaccgg
      121 ataaattcca ggcagtcggc gttgattaac tcacaactgg atatttttac agtattaagc
      181 atggatcatt aagcctgtct ctgataggct cattctgctt ttgcgcaaag cagtgggcct
      241 gaggtttgct tgtgaaccca acgcatgagc agatggctgg tgggtgcccc taacacccac
      301 cagccgccca tttaccacaa ataaaaaagc cttcactgcg gaaggcgtct gtaacaaccg
      361 aactgatagt ctgccagacc cgccataacc agctgggtca gtattaactg gcagcgttcg
      421 cgtgaaaggt aagtattctg tgcaatctcc ccgactgtcg ccggttcggt aacgcttaat
      481 tcattaaaca ccactctggc ggtttctgtc atatcctgct gttttagcat gtctttttcc
      541 cttttccggt taacgtgaca caccaataac tcttgtcgaa aaagccagca agctgaaaga
      601 caggtattca ccgccaccag cgcgtttact gtactgacgc gatttcagtc ataaaaaacc
      661 cgccaggcgg cggggtgtaa aaaatcttct aacgtcaggc ataaaacgcc catcgttaga
      721 gcaaatttac cacagattcg ggaaaaatca acaacactat cgcgttaccc tctttaactg
      781 ccgctccgcc catgcctctt caatgtcaaa ccgaaccacc aacgtatcgt aaaagcgttt
      841 cactgatttt ttccacgtat caagcgtgat agcactcgtc actttgcata tggcattaaa
      901 tgcctccgtt gatggtagtc tttcacagcc acgaccacca caacgctggc agtctctgat
      961 aacaggcata ccacgtttta ccgactcttc acgatgaatg gcgacaccac gcccacggca
     1021 atccttacag gcggtggaaa cctcaccctt tccgccacac tccggacagg caacttttac
     1081 cacctccctg acttttttcc attcttccca gtaagacgga tacacacctt tcgtacactt
     1141 tgcccatacc ggcggcttac catccggata ctggaccttg tttgtaaaaa ctacgctttc
     1201 aataaatttt tccccatagc aacaagggca ctgcttttta ctcgctgcgc tgcgggcata
     1261 atcctcaaaa gcgtacgaag ccataatgcg catcactacc ggttttattt ctgccgggag
     1321 ttttcttaac gccgccacgc gatcacaccg actgagtgca tattctgtca gcaattctgt
     1381 tgcccgctct ctgtcattca tactaatgcc cattttccca aggaacgcag aaaaccccat
     1441 ctcagcccga ttctgtgtca tgccctgcgc ggccatcaca tcagtgatac tcagcgcatc
     1501 tttcgacgtt gaggccgatg catcagtcag gccgggggat tttggggagt agtatttcgg
     1561 taaatcttcc agtttcattt tttgacctgc ccttcaagca ttatggggta aatcttcacc
     1621 cccagacgtc caccagatac tggctgagca cgaacgatat tgatttcatc aaactgctca
     1681 tcgtccatta gcaaccccgc atgcgtcagc gcatccagcg gcgctttcag aatattgtcc
     1741 aggtcacggc ggcgcttatc cggtggttct gcaataattt ttattgccaa ccttccggac
     1801 aggcttaatt tcagccgctg ctggcgaaca ataagcgcca ctgcccggcg ataacgctcc
     1861 ccggcttttg atacaaaata tgtgctgcca cgacgacgcc agtaagtgtt caccgttggc
     1921 gggtaaggca aaacaaattc tatgcgttca gtcatttatg ctttccactt cagaacaccc
     1981 gaatttctcg cgtgcattaa aaaacgaatc agcaacaaca gctggctgcc gtgtttttct
     2041 tcaaaatctt ttaccccggc gtgtagttcg ctatggcatt tacggcacag cggaataaca
     2101 aacaaatcat cagcctttgt tcccatccct cccagtccat gaccaatgat gtgatgcgga
     2161 tcatctgcct gattgccaca cgtcatgcat ttctgcgttt ttacccaacg cgtgtataca
     2221 ggcatctctt cccgttgtga tttctggcgc tggagatact gagccggtga ctccggatca
     2281 acggcaatgc tgaccaccgt cttttcctgt ggcgggtttt gctggtgggc gtgaggcagc
     2341 ggcgcaagat tttttgtgcg ctgcttcagt atgctggtgg cggtctgctc tcccggtacg
     2401 atgtcgcttt cacggtacat tgagcggatt ttttccgcac gcaaccccag cgaacgacgt
     2461 aataccgctt ccggtagcgc gtccgccacc tgattgcgga ccgcccacca ggataattca
     2521 gccagcgata attcccgttc ctgcgagcca ttcattgcat ggcgtatgac gtcaatcatc
     2581 catgcaaaca ggttttggtg agcaagttgc ccgagtgatt cggaggtctg gtcgcgcagc
     2641 tggttgtcgc agtgccagca caacaccatt gcgccggtac cataacggtg aatgacggtt
     2701 tcactgtggt gataatcgcc gtgtggccac tggcaggatt taacatggcg cagtaaccag
     2761 tcagacaatg cgccagcgcc accagcagca cgaatcactc gttcgtcgct gaaaaatggc
     2821 agtaatgatt tatcctccgc cagcggctgg cgaacggcag gaacgacccc ggacggcaga
     2881 ttacgcatgc ttttcggttc cggctccacc agtacccggg tattgtggaa taccggcatg
     2941 gattcacggc ccggcttaac gatcaccagc ccgagttccg gtaccagaac aggtcgaagt
     3001 aatacccgca cgttacctcc agatgcgttg ctggaatgtg cgggacggac gcggtgggcg
     3061 ttcagagtaa ggaagcctga cggagattat ccagtgacga taatcgaggc tgagggcttt
     3121 cttaatctcg tatccgcgtc tgcggtagtt atgaattagc cattcggcct gttcttcagt
     3181 acatggtggg tgttggtacc agtcggtttt aaatgcgtgt gaacgccgcc catgccggat
     3241 ggcaaggtcg gtatcagaat tgtgaaattt ggttttgtgc gccatctgtt ttctctgctg
     3301 gcgcagcagg tgtcaggtgt tcaggctgac gtgcgaattg taaaccagaa tgccagaaaa
     3361 aaacaaaacc cgccgaaccg ggttaagtgc gggtgcgttg aggatgcctg acacatcaga
     3421 ggtggcgagg gatttctccc ccgccaggtc tcttactcct caggttcgta agctgtgaag
     3481 acagcgacct ccgtctggcc ggttcggatt cgtacctcgc agaggtcttt cctcgttacc
     3541 agtgccgtca ctatgacggt taaacagatg acgatcaggg cgattaacat cgccttttgc
     3601 tgcttcatag cctgcttccc cttgcctttc ggcacgtaag aggctaacct acatttgtga
     3661 gacatagatt gggcctcaga ttaatgttaa gcgtcttgca ggacgcgaaa tgttaactgg
     3721 ggcttttctc tatctgcctt tcagtgttca tgcctgagac agatagcctc aagcacccgc
     3781 agccattcta cttaactcac gtcacctcgc caatatgaaa tcaatcagaa aggtgatcca
     3841 taaaatcact ccttctcttc ttttccgtag tggagttggc caattttgat aagagggcgt
     3901 ccctgagatt tgcggtgtag attggtatcg cgcagagaat acacacagcc acaatattcc
     3961 tgctgataga atttttcgcg cttgctgatt tcaatcatac gggacgagcc gccctgcttg
     4021 cgccagttat aatcccagta caccataccc ggataatgcg caacagctcg ccgcccacac
     4081 tcgttaacct gctgcatatt tttccagcgt gaaatgccca gtgaactgct gatcacactg
     4141 aaaccatttt cagcagcgta caacgctgtc cgctcaaaac gcatgtcaaa acacatggta
     4201 caacggatcc ccctctcagg ctcccattcc attcctttgg cacgttcaaa ccagttgtcg
     4261 gtgtcgtaat cagcatcgat aaacggcacg ccgtgttgtt cagcaaagcg aatattttca
     4321 tccttacgaa ttaaatactc tttctgagga tgaatgttcg ggttgtagaa aaagatggtg
     4381 tagtcgattc ccgaggcctg aagcgcctcc atcacttcac cggaacatgg agcacagcaa
     4441 gagtgcagta gtagtttgtt tgccccgttt gggagctcca atttaggccg tttgaaatca
     4501 gcaatagtca taaatatttt tattggggtc atgaaaatag cacagagtgt agcatcagag
     4561 cagggctatc gggaatatat gtctaaatct ggtaatatct ggttttgacg caaagcggac
     4621 aaccacgctg gctctaccct gcgccatgaa aatgtcaatt cacatctgaa ctaatgctct
     4681 ttaatctagt aacgtctaaa atacctaaca tttccttgat aaaatgccag tacacgctgc
     4741 atagcttcgc tcttccggca ctcgcgacag attatattca ggcgcctgtc gtagcggcgt
     4801 atttcgccgt ctggtaacga ccagataagg tccggatcaa ccactgcagg tttcttcacc
     4861 tttgcccttg agagtttttt gcgagcattt tgccagtcct tacgcgcctg ttcagacggg
     4921 aataacccgt aaccagagtt gtatacatcg ccactggcaa ccagctctct ggcgagaaca
     4981 ctcatcagat atcttgtcgc acctgtcttg gcttccagtt gccgcaacgt ctcgcgaccg
     5041 ctcagacgta caagttcaac aacctgccct ttaatttttt cccgctcttc ttgtgtaaat
     5101 acttttgcca taagcgcctc cggcaatcac ttttccgata caacacggcg ggaagaatca
     5161 gtaatctgtc gaacaatatc ccggtgcttg ttcagctccc gcagcgcggc gcagactcgc
     5221 tcccacttct gaacatcact tttcgccctg cgcagcgcca ggtttgccct gcgaagggac
     5281 ggaaaaatca gctcatctgc ttgcgtttcg gtaaacgatg gcaacggctg cacaatgtcc
     5341 gccacagttt ctgttttaat ttcttcctgt gttgcggctt cccggactgg taacgcagca
     5401 cctgctggct gaggaaaggc cttaccatca ctttccgtta ccagcgcggc tttcggctct
     5461 gctggtaaat tatcgcccgg catgcagtaa cgaaatttac cgttctgatt aacgcgtgcc
     5521 agccgccccg ttgcggttac caccgccagc gtggaagcaa ccttgcgagt actgacaccg
     5581 aacttacccg ccagttcctc acacgtttta gccccatcct gaccgataaa ctcaatcatc
     5641 atgtctgcgg taactttttg ttcgacctcc ccggtcagca tatcctgtgc ttcagatttt
     5701 actggccgct cttcggttac ccgggattca ccttcgccag ccagaaacca ggtgtgacca
     5761 gttttatcaa cgacgccttt tcttttgagt tcccacagct cgttgacagc ctcttcacga
     5821 ctgattccaa ggcgagctgc caccacatgt gaagaggctt ttttcagtgc tttcagtgcg
     5881 tcagatacgg tttccattaa aatttcctcc ggacaaaatt acttcacaac cctcatattg
     5941 ctgacatttg gacgccagct atcccagtta aacgtcaccc atcgaccacc gttcatggtc
     6001 atgcggtcca taatcctctc accaagaagc gtactcattg cggcatgatt caggtttgtt
     6061 aacatcccga cactgcacag tgatgctgtc cggcgatcaa ttatctggtg caataccacc
     6121 tgctcgtttt tcgtctcccg ctgaacgcct atttcatcca ggaccagcaa atcaaccccg
     6181 caaagctcct gtatttttcc ccggatttgc cgttgtcgta gctgtcatgc aacacgctca
     6241 tgacgtcaga cacggtgacg ataatcacgc tgcgcccctt caccatcagc cggttgccca
     6301 tcgccgctgc aaggtgattt ttcccggtgc cggttttacc gctgaacaca aaattcgtgc
     6361 acccggtcat cagttcgtca gctatggatt tggcctggct cagcgcgtat ttttgcccgt
     6421 cgttctgcac ctgataattt gcaaacgagc atttgctgtg cagaggctgg atgcccgaac
     6481 gattcaggat tttttccacc cgcaactggc gattctggcg gttaatctcc tcgctgcgtt
     6541 ttcgtccttc agcaagttgc cattcccgcc actcctccac cgtccggtac ggtggaaccg
     6601 acccctgtgg tgcaagtctg cgaatacgtt caagaacccc aactgccgca atgtttttca
     6661 tgacacgtca ccccctgaat cccggcggta tttcagtgtc cggttcagaa atgtgattca
     6721 cacaacgctg cgcaggcgaa cgccccaggc ggataaccag ttcatcccat ttttcccgga
     6781 gttttgccgg actcatgatg ttttttaccc agaacgaatc ccgctggaga cgcccaaaca
     6841 tttcacaaat ttgtctgtga gttctgccat ccagcatccg cattgtgcga acgtcattgg
     6901 cccatgctgt ccagttgggt tctttcggtc tagtgatctc gccatcatag ctggccgcct
     6961 gctcgtaaag actcacgatt cgtccccaga tccactgtgc gcacaccaaa tcttcctgac
     7021 ttccccactg gcgttttttc gcactgaaca caaccgcgtc agggtgtcgg gttaaaaaat
     7081 cctgttcagc cgtctgcggg tccggttgcg aagcgtccgg acaagaagat cttttatctg
     7141 acggatcagg ttttaatact gacggatcgg ggtcaatcat cgccccccta atcggcagtt
     7201 ttttatcaac agttgatcca tcaaaatttg acgggtcaac cgttgagggg tcaatatttg
     7261 acgggtcaac tgttaacggg tcattttttg ccgggctaat ttttcttttc ggtttatatg
     7321 actcacgcgc cgccgccgca gctgcttcga gtttttccac attaagccga tagatattgc
     7381 ttacattacg cccaccgacc ttacgctctt ccttcgtcag ccagccctct ttcgccagtt
     7441 ctgcaatagc cgatttcact gtggattcac ttcttgcacc gatctgacgc cggatagttt
     7501 caatggcagg ccatgacacg ccctcgtcat tgctgtagtc tgcaagacgg gccataaccg
     7561 ccaccctgga taagatcatg ccggtgaagg cgcacccttc ccagacaaga ccatgaagct
     7621 tgctgctcat aaaacccccg aacaccgtgc ttttagtgca tcaccacagc attccctgcc
     7681 gggccgccgc gattcatctg gtcatacaaa acaaccgctg acgcaacaaa atcatcgaca
     7741 tccttcacca gccgatccct ccgttcgacg atctcacggt aatattcaga actgtggctg
     7801 cgcatacggg ccaccagcaa aggcggcatc gccttttcga tcgccggtaa cagagcctgc
     7861 attttttcaa cagcatcagg ggggtcttta tccagccaac ggaaaatttt ctgggtatta
     7921 cgggccaggg cttccggatg gctgtcgtcg tacagttcag ggaacgtcat ccccagttcg
     7981 aaataagtcc gggctatttc agctgcagga actttctcac catcagggta tgcccaggca
     8041 ttcatcgcca tgcggatgtg ctcatgtttg attttcatga atcatttgcc tcttgatgct
     8101 tcgggtatga tcgttttcgt catttggttg cttcatcgac atattctgcg aataacatga
     8161 cgagcgtcgt aagtatgtcc aatcaacatc aggacgaagt tcttcacaca ggacaccacc
     8221 ttttgttgct cgttcaatcg caggacatct ctcagcaggt aactgacgta cacctttgat
     8281 ccattgattt acgcttggag gagatacacc taaaagccta gccattgctg attgcccacc
     8341 gacaacagca caagctcgtt tgaatgaata gttatctttt ttcatcgaat gaactccaaa
     8401 aaacacgcaa caatattagg cttagcctaa tgaaattgtc aataggctat gcctaataca
     8461 tcgagagtag ggattgccta acgcgatgcg cataggagac tattaagcaa tgcttagtgg
     8521 taaagactta ggccgagcga tagagcaggc cattaacaaa aaaattgcat caggagccgt
     8581 caaatcaaag gcggaaatcg cacgtcattt caaagtccaa ccaccatcaa tccatgactg
     8641 gattaagaaa ggttcgataa gtaaagacaa acttccagaa ctatggcgtt tcttttctga
     8701 tgtggttggt ccagagcatt gggggcttaa cgaatacccc ataccaaccc catccacttc
     8761 agatacaaaa agtgaacttt tagacataaa cagcctttat caagccgcct ctgatgaaaa
     8821 aaaagcaatt gtggctttca tcttatctgg aaatgctacg gagcctagtt gggttgatta
     8881 tgacgttcgc gcctacattg ccgcaatgga aatgaaggta gctaactatc tgaaaaatca
     8941 agaatcaaaa cggaaaagcc agaacatcac caagacagga acttaaactt atatggtccg
     9001 acgggaaatt cctagttccc gttagttaac tcctactacc tctcccacaa accatcacct
     9061 attaggttgc gcccaaatta ttaggcatag cctattgaca agtaattagg catttcctat
     9121 agttttccca taccaaccca tcccgtccca cacaatacag ggcaatacct cgagttacca
     9181 ggcagtggtc aggggttaag tagccagccc gaggcgtaag aacatgacgg cagggttcaa
     9241 ctttaataac tatgcagcag gtttttgttc cgctaccccg gcgttaaggg gaaatgaggt
     9301 cagcatggat actatcgagc ttggcaacag cgaatctctg gtatgtggcg tgttccccaa
     9361 ccaggacggt acgttcaccg cgatgacgta taccagaagc aaaacgttta aaactgaagc
     9421 tggcgcgcgt cgctggttaa ccagaaacac tgactgatga ggttgacgat ggaatttaaa
     9481 gatttaccag taccattcca ggaaatggca tcgaatgtgg ttcgctctca actggcgact
     9541 cttgacctga gtaccgtaga aaaagaaacc atcgacaata tatccggtaa cgtacgccga
     9601 gcctttatcg ggctgtacga agagaagcag ctctctgata accaggattt acatgaaaaa
     9661 tacttcctgg atctaatgga catcattgat aaggggtttg gcttgttaat gaaaaagaaa
     9721 gggattcgaa tagaacccct tgaaaattac tttgcaacaa aaagcattaa ttcttttgat
     9781 tcaaaataag agaattaatt acagacttaa catgctcttt ctcatgattg aagctctcat
     9841 gattgaaagt gccgggttga agcgagtcga tataatcaac aagactctgt cgtacgactt
     9901 catttttatc cataacagat gcaagaaatg aaattgctaa aagagttata tcactacgcg
     9961 ccgcagcatg ttgcaatgct ttatcaaaat tattaatctg gcgtatcagg gagttaatga
    10021 tttcatcatt ttcagtcgac atttcaccct cctgagggtt ggtgattaag gagttctcca
    10081 cgggtgaggt ggagtgcgtg cgccggacac gggtgagcat ccggcactga cagtttactg
    10141 aaaggatatt tccctgaaaa gtcagaccat aacgcgaaag cgcccggcga ggtagctggt
    10201 tcatagatag cctgtcgtta aattttcgtc gaccgtgcgt ttccggttgt ggcaatccgc
    10261 gaaatggcgc ggcggtaagt atggcggggt tattccttcc ccgttgagga caccgagttg
    10321 tcaggttgac catacgctta agtgacaacc ccgctgcaac gccctctgtt atcaattttc
    10381 tggtgacgtt tggcggtatc agttttactc cgtgactgct ctgccgccct ttttaaagtg
    10441 aattttgtga tgtggtgaat gcggctgagc gcacgcggaa cagttaaaac caaaaacagt
    10501 gttatgggtg gattctctgt atccggcgtt aattgttaac tggttaacgt cacctggagg
    10561 caccaggcac cgcatcacaa aattcattgt tgaggacgcg ataatggaaa cgttattacc
    10621 aaacattaat acgtctgaag gttgttttga aattggtgtc actatcagta accctgtatt
    10681 tactgaagat gccattaaca agagaaaaca cgaacgggag ctattaaata aaatatgcat
    10741 tctttcaatg ctggcccgtt tacgtccgat acaaaaagga tgctggcaat gaatacagca
    10801 tttgcacttg ttctgacagt ttttcttgtt tccggagagc cagttgatat tgcagtcagt
    10861 gttcacagga caatgcagga gtgtgtgact gcagcaaccg aacagaaaat tcccggtaac
    10921 tgttacccgg tcgataaagt tattcaccag gataataacg aaatcccggc aggtctttaa
    10981 aacagttccg taataaatat ccggtttcat tcttatatgc cagcaatggc agggatttgt
    11041 tcacccttaa atctgtaatg aggtaaaaca aaatgagtaa agtctttatt tgcgccgcca
    11101 ttccggacga acaggcaata aaggaagaag gtgccgtcgc tgtagccact gccattgaag
    11161 ccggtgatga acgtcgcgcc cgcgcaaaat ttcactggca attcctggaa cattatccgg
    11221 ctgctcagga ctgcgcttat aaatttcttg tctgcgagga taaacccggt ataccccgcc
    11281 ctgccctcga ttcctgggat gctgaatata tgcaggaaaa ccgctgggat gaggagtctg
    11341 cttcctttgt cccggttgag actgaatcag atccgatgaa cgtcactttt gacaagctgg
    11401 cccctgaagt acagaacgct gtcatggtta agttcgacac atgtgaaaac atcaccgttg
    11461 atatggttat tagcgcacag gaattgttgc aggaagacat ggcaacattc gacggacata
    11521 tcgttgaagc gttgatgaaa atgccagaag ttaacgccat gtatccggag cttaagctgc
    11581 atgccatcgg gtgggttaag cataaatgta agcctggtgc caaatggccc gaaattcagg
    11641 cagagatgcg catctggaaa aaacgtcgcg aaggtgaacg caaggaaacc ggaaaataca
    11701 cgtctgttgt tgatctcgcc cgcgccagaa ccaatcaaca gcacagtgaa aattcaacag
    11761 gaaaaatcag cccggtcatt gctgccattc atcgcgaata caagcagaca tggaaaacac
    11821 tggatgacga actggcctac gctctctggc ctggtgatgt ggatgccgga aacattgacg
    11881 gcagcatcca tcgctgggca aaaaatgaag ttatcgacaa cgaccgcgaa gactggaagc
    11941 gtatctcggc atcaatgcgc aaacagcctg atgcccttcg ctacgaccgc cagactattt
    12001 ttggccttgt ccgtgaacgt ccgatcgaca ttcacaaaga ccctgtggca ctgaacaaat
    12061 acattactga atacctgact acaaagggcg tgtttgaaga tgaaggaaga aatcagagcg
    12121 caactgatac tctctcgtcg ccagtaccag aaactgatgc agtggaaacg gcaattccgg
    12181 gcaacgaaaa aaccgaatgc aaagtggaag tcgaaccatc tgtagagcgt gaggggccgt
    12241 tctacttcct cttcaccgac aaggatggcg aaaaatacgg tcgcgcaaac aaactttctg
    12301 gtctggaaaa agcactagcc ttgggagcta cggaaatcac aaaagaggaa tacttcgcac
    12361 gtaaaaacgg cacgtactca ggttcacaac aaaatactgg tgcatctgac acgaccgcac
    12421 aaccagagcc ggtaaaagtt accgctgacg aagtaaacaa aattatgcag gcagccaata
    12481 tcagccagcc tgacgccgat aagttgcttg ctgcctctcg cggagaattt gttgcaggga
    12541 ttagcgaccc gaatgatccg aaatgggtaa aggggattga aacccgcgat tctgtaaacc
    12601 agaaccagca agaatcggaa cagaacgacc agaaagcgga acaaaacagc ccaaatacgc
    12661 aacaaaacga gccagaaacg aaacaacctg agccagtagc gcaacaggaa ccggaaaaag
    12721 tctgcaccgc ctgcggtcaa agcggtggcg gcaactgccc tgattgtggc gcggtgatgg
    12781 gtgacgcaac ataccaggaa acattcgatg aagagaatca ggttgaagtt caggaaaatg
    12841 atccgaagga aatggaaggc gctgaacatc cacacaagga gaatgctggt agcgctcagg
    12901 atcacgccag cgatagtgaa actggcgaga cggcagatcc cttaattgcg atgaacggtc
    12961 atcacgttat cacatccacc agcaggacgt gtgaccatct aatgatcgac cttgaaacca
    13021 tgggaaaaaa tcctgatgcc ccgattatct caataggtgc aatatttttc gatccgcaaa
    13081 ccggagatat gggaccggaa tttagtaaga ctatcgatct ggaaactgct ggcggagtca
    13141 ttgatcgtga cgtcattaaa aggtggctga agcaatcacg tgaagcgca
//
LOCUS       DAJTOR010000113        13087 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTOR010000113 DAJTOR010000000
VERSION     DAJTOR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.113"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            176..1150
                     /gene="cysB"
                     /locus_tag="OO249_002850"
     CDS             176..1150
                     /gene="cysB"
                     /locus_tag="OO249_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5637081.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QTRYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1445..1609
                     /locus_tag="OO249_002851"
     CDS             1445..1609
                     /locus_tag="OO249_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5637082.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1612..1779
                     /locus_tag="OO249_002852"
     CDS             1612..1779
                     /locus_tag="OO249_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637083.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1893..1988
                     /locus_tag="OO249_002853"
     CDS             1893..1988
                     /locus_tag="OO249_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637084.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2152..4827
                     /gene="acnA"
                     /locus_tag="OO249_002854"
                     /pseudo
     CDS             2152..4827
                     /gene="acnA"
                     /locus_tag="OO249_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
     gene            complement(4891..5481)
                     /gene="ribA"
                     /locus_tag="OO249_002855"
     CDS             complement(4891..5481)
                     /gene="ribA"
                     /locus_tag="OO249_002855"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5637085.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5651..6415
                     /gene="pgpB"
                     /locus_tag="OO249_002856"
     CDS             5651..6415
                     /gene="pgpB"
                     /locus_tag="OO249_002856"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5637086.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLLWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6564..6872
                     /gene="lapA"
                     /locus_tag="OO249_002857"
     CDS             6564..6872
                     /gene="lapA"
                     /locus_tag="OO249_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5637087.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            6879..8048
                     /gene="lapB"
                     /locus_tag="OO249_002858"
     CDS             6879..8048
                     /gene="lapB"
                     /locus_tag="OO249_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5637088.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNRARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8241..8978
                     /gene="pyrF"
                     /locus_tag="OO249_002859"
     CDS             8241..8978
                     /gene="pyrF"
                     /locus_tag="OO249_002859"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5637089.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8978..9304
                     /gene="yciH"
                     /locus_tag="OO249_002860"
     CDS             8978..9304
                     /gene="yciH"
                     /locus_tag="OO249_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5637090.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9430..9648)
                     /gene="osmB"
                     /locus_tag="OO249_002861"
     CDS             complement(9430..9648)
                     /gene="osmB"
                     /locus_tag="OO249_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5637091.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9917..10666)
                     /locus_tag="OO249_002862"
     CDS             complement(9917..10666)
                     /locus_tag="OO249_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5637092.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(10756..10929)
                     /locus_tag="OO249_002863"
     CDS             complement(10756..10929)
                     /locus_tag="OO249_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637093.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(11076..13061)
                     /gene="pdeR"
                     /locus_tag="OO249_002864"
     CDS             complement(11076..13061)
                     /gene="pdeR"
                     /locus_tag="OO249_002864"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5637094.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKSEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         3329 a         3081 c         3291 g         3386 t
ORIGIN      
        1 tcgcgaaagc ggcccttttt tattgcatat tattttttct tcacacctat acactaaggc
       61 tataaatgat atagtggtta tagttagcac cttttttatt attaaatcgt attagtcacc
      121 cgccaggtgt gacgaaaaaa cgatgttctg atggcgtcta agtggatggt ttaacatgaa
      181 attacaacaa cttcgctata ttgttgaggt ggtcaatcat aacctgaatg tctcatcaac
      241 agcggaagga ctttacacat cacaacccgg gatcagtaaa caagtcagaa tgctggaaga
      301 cgagctaggc attcaaattt tttcccgaag cggcaagcac ctgacgcagg taacgccagc
      361 agggcaagaa ataattcgta tcgctcgcga agtcctgtcg aaagtcgatg ccataaaatc
      421 ggttgccgga gagcacacct ggccggataa aggctcactg tatatcgcca ccacgcatac
      481 ccagacacgc tacgcattac caaacgtcat caaaggcttt attgagcgtt atcctcgcgt
      541 ttctttgcat atgcaccagg gctcgccgac acaaattgct gatgccgtct ctaaaggcaa
      601 tgccgatttc gctatcgcca cagaagcgct gcatctgtat gaagatttag tgatgttacc
      661 gtgctaccac tggaatcggg ctattgtagt cactccggat cacccgctgg caggcaaaaa
      721 agccattacc attgaagaac tggcgcaata tccgttggtg acatatacct tcggctttac
      781 cggacgttca gaactggata ctgcctttaa ccgcgcaggg ttaacgccgc gtatcgtttt
      841 cacggcaacg gatgctgacg tcattaaaac ttacgtccgg ttagggctgg gggtaggggt
      901 cattgccagc atggcggtgg atccggtcgc cgatccggac cttgtgcgcg ttgatgctca
      961 cgatatcttc agccacagta caaccaaaat tggttttcgc cgtagtactt ttttgcgcag
     1021 ttatatgtat gatttcattc agcgttttgc accgcattta acgcgtgatg tcgttgatgc
     1081 ggctgtcgca ttgcgctcta atgaagaaat tgaggtcatg tttaaagata taaaactgcc
     1141 ggaaaaataa tttcttgcgt tattttcggc accttttatg tagcgaaggt gccggaatat
     1201 attctctttt gttacttatt tttataccgt gcacaatatt tttatccctc atatttatag
     1261 ggtaaattac ctgtaaagta gtgtctaatt acccgcctcg caaattccag ttttcaaata
     1321 tctatttcag gtcaaaagat tgaattattc actggagacg attcgtaaat tcgctggatt
     1381 tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc aatcgttcaa
     1441 cgtcttgctg ataagtgatg gcgattatat gaggttagca atgccttctg gaaatcagga
     1501 accccgccgc gatcctgaat taaaacgtaa agcctggctg gcggtttttc ttggttctgc
     1561 acttttctgg gtggttgtcg cactgctgat ttggaaagtg tggggataac tatggtcggt
     1621 caggagcaac tggagtcgtc accattatgc cagcatagtg acaatgaaac agaaacgaaa
     1681 cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca gcaagccttt
     1741 atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt cttattaccc
     1801 atttttaatg aattaaaggg cttttaatac accgcagcaa taacagcttg agttatctca
     1861 acacaaaata ataaccgtta agggtgtagc ctatgatcaa cacaaatatg aaatattggt
     1921 cctggatggg cgcgttttct ctgtcgatgc tcttctgggc cgaactcctc tggatcatta
     1981 ctcactgatc cttgaccccg ctgcggcggg gttgtcattt gctttgccac aaggtttctc
     2041 ctcttttatc aatttgggtt gttatcaaat cgttacgcga tgtttgtgtt atctttaata
     2101 ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt aaggaggagc tatgtcgtca
     2161 accctacgag aagccagtaa ggacacgttg caggccaaag ataaaactta ccactactac
     2221 agcctgccgc ttgctgctaa atcactgggc gatatcaccc gtctacccaa gtcactcaaa
     2281 gttttgctcg aaaacctgct gcgctggcag gatggtaact cggttaccga agaggatatc
     2341 cacgcgctgg caggatggct gaaaaatgcc catgctgacc gtgaaattgc ctaccgcccg
     2401 gcaagggtgc tgatgtagga ctttaccggc gtacctgccg ttgttgatct ggcggcaatg
     2461 cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg ttaacccgct ctcaccggtc
     2521 gacctggtca ttgaccactc ggtgaccgtc gatcgttttg gtgatgatga ggcatttgaa
     2581 gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt atgtgttcct gaaatgggga
     2641 aagcaagcgt tcagtcggtt tagcgtcgtg ccgccaggca caggcatttg ccatcaggtt
     2701 aacctcgaat atctcggcaa agcagtgtgg agtgaattgc aggacggtga atggattgct
     2761 tatccggata cactcgttgg tactgactcg cacaccacta tgatcaacgg ccttggcgtg
     2821 ctggggtggg gcgtgggtgg gatcgaagca gaagccgcaa tgttaggcca gccggtttcc
     2881 atgcttatcc cggacgtagt gggctttaaa cttaccggta aattacgtga aggtattacc
     2941 gccacagacc tggttctcac tgttacccaa atgctgcgca aacatggcgt ggtggggaaa
     3001 ttcgtcgaat tttatggtga tggtctggat tcactaccgc tggcggatcg cgccaccatt
     3061 gccaatatgt cgccagaata tggtgccacc tgtggcttct tcccaatcga tgctgtaacc
     3121 ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg ttgagttggt cgaaaaatat
     3181 gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac cgatttttac cagtacgtta
     3241 gaactggata tgaatgacgt tgaagcgagc ctggcagggc caaaacgtcc acaggatcgc
     3301 gttgcactgc ccgatgtacc aaaagcattt gccgccagta acgaactgga agtgaatgcc
     3361 acgcataaag atcgccagcc ggtcgattat gttatgaacg gacatcagta tcagttacct
     3421 gatggcgctg tggtcattgc tgcgataacc tcgtgcacca acacctctaa cccaagtgtg
     3481 ctgatggccg caggcttgct ggcgaaaaaa gccgtaactc tgggcctcaa gcggcaaccg
     3541 tgggtcaaag cgtcgctggc accgggttcg aaagtcgttt ctgattatct ggcaaaagcg
     3601 aaactgacac cgtatctcga cgaactgggg tttaaccttg tgggatacgg ttgtaccacc
     3661 tgtattggta actctgggcc gctgcccgat cctatcgaaa cggcaatcaa aaaaggcgat
     3721 ttaaccgtcg gtgcggtgct gtccggcaac cgtaactttg aaggccgtat ccatccgctg
     3781 gttaaaacta actggctggc ctcgccgccg ctggtggttg cctatgcgct ggcgggaaat
     3841 atgaatatca acctggctgc tgagcctatt ggtcatgatc gtaaaggcga cccggtttat
     3901 ctgaaagata tctggccatc ggcacaggaa attgcccgtg cggtagaaca agtctccaca
     3961 gaaatgttcc gtaaagagta cgcagaagtt tttgaaggca cagcagagtg gaaggaaatt
     4021 aacgtcacac gatccgatac ctacggttgg caggaggatt caacctatat tcgcttatcg
     4081 cctttctttg atgaaatgca ggcaacacca gcacctgtgg aagatattca cggtgcgcgg
     4141 atcctcgcaa tgctggggga ttcagtcacc actgaccata tctctccggc gggcagtatt
     4201 aagcccgaca gcccggcggg tcgatatcta caaggtcggg gtgttgagcg aaaagacttt
     4261 aactcctacg gttcgcggcg tggtaaccat gaagtgatga tgcgcggcac cttcgccaat
     4321 attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg ggatgacgcg gcatttacct
     4381 gacagcgatg ttgtctctat ctatgatgcc gcgatgcgct ataagcagga gcaaacgccg
     4441 ctggcggtga ttgccgggaa agagtatgga tcaggctcca gccgtgactg gacggcaaaa
     4501 ggtccgcgtc tgcttggtat tcgtgtggtg attgccgaat cgtttgaacg aattcaccgt
     4561 tcgaatttaa ttggcatggg catcttgccg ctggaatttc cgcaaggcgt gacgcgtaaa
     4621 acgttagggc taaccgggga agagaagatt gatattggcg acctgcaaaa tctacaaccc
     4681 ggcgcgacgg ttccggtgac gcttacgcgc gcggatggta gccaggaagt cgtaccctgc
     4741 cgttgtcgta tcgacaccgc gacggagttg acctactacc agaacgacgg cattttgcat
     4801 tatgtcattc gtaatatgct gaagtaacaa gtatttgctt gccggttatt ttgcttccgg
     4861 caagcaaatt aattccacaa tgcgagaggg ttatttgttc agcaaatgac ccattttctc
     4921 ggctttggta tcgagataat gttcgttatt ggggttacga cctacaatca atggtacgcg
     4981 ttcaacaata ttaatccctg cttcggtcag aatttcgact tttttcgggt tattggttaa
     5041 caagcggact tcattgacgc caaggagttt gaacatatca gcgcaaagag tgaagtcgcg
     5101 ctcatcagcg gcgaagccta actggtggtt agcctctacg gtatcgtaac cttgatcctg
     5161 cagtgcgtaa gcgcggattt tattcagcag accaatgtta cgaccttcct gacggtgata
     5221 cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat gccgcttcga gctggaagcc
     5281 acaatcgcag cgcaagctga acagggcgtc accggtcaga cattcggaat ggacgcgcgc
     5341 aagtaccggg gtatgcccgg aaatatcgcc atagactagc gcgacatgat cgtgtccggt
     5401 tgccagttct tcaaatccca ccatcaggaa atcgccccat ggggttggca gtttggcttc
     5461 tgccacacgt ttaagctgca tgaaattctc cagataatgc tggttctgta ttggcttatt
     5521 ttgccataac gagaaggggt tcacctaatc acgacgcgtc gatcgttcac ggaatggcac
     5581 aaatctgtca atttttcctg gaactggcgt tttcagttat gattgtggga cttatcaaaa
     5641 aggagaggcc atgcgttcga ttgccagacg taccgcagtg ggagctgcac tattgcttgt
     5701 catgccagta gccgtatgga tttctggctg gcgttggcaa cctggagaac aaagttggct
     5761 actaaaagcg gctttttggg ttactgaaac tgtcacccag ccctggggcg tcattacaca
     5821 tttgatttta ttcggctggt ttctctggtg tctgcgtttt cgcattaagg ctgcctttgt
     5881 attatttgcc attctggcgg ccgcaatcct tgtgggacaa ggcgttaaat cctggatcaa
     5941 agacaaagtc caggaaccac gaccttttgt tatctggctg gaaaaaacac atcatattcc
     6001 ggttgatgag ttctacactt taaagcgagc agaacgcgga aatctagtga aagaacagtt
     6061 ggctgaagag aaaaatatcc cacaatattt gcgttcacac tggcagaaag agacggggtt
     6121 tgcctttcct tccggtcaca cgatgtttgc tgccagttgg gcactgctgg ccgttggttt
     6181 gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg ctgctctggg caacgggagt
     6241 catgggaagc cgcctgctgc tcgggatgca ttggccacgc gatctggtag tagctacatt
     6301 gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg caacgaattt gtgggccatt
     6361 aacaccacct gcggaagaaa atcgcgaaat agcgcaacga gaacaagaaa gttaacgctg
     6421 gttgattttc cgaatttagc ccttaaatca tcaacaatgc gcgtggatgc catttcgcag
     6481 acggcgcgaa aatggtactt taaagggcta ttgcggtaag ttgaccataa tttattcgct
     6541 ctaaccacat aacgggaagt aatgtgaaat atttactcat tttcttactg gtgttagcga
     6601 tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca acaggtgacg tttaattatc
     6661 tgttagcgca aggggagtac cgtatttcca cattgctggc ggtattgttt gctgcggggt
     6721 ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg agttcgtgtt tccctggcgc
     6781 gcgctgaacg taaaataaag cgactggaaa accagctttc acccgcgact gacgtggctg
     6841 tagtgccgca ctcgtcagcg gcgaaggaat aactttctat gctggagttg ttgtttctgc
     6901 ttttgcctgt agccgctgcc tatggctggt atatgggccg cagaagtgcg caacaaaaca
     6961 agcaagatga agccaaccgc ttgtcgcgtg attacgtagc gggggttaac ttcctgctta
     7021 gtaatcaaca ggataaagcg gtagacctgt ttctcgatat gcttaaagag gatacaggca
     7081 ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc gcgtggcgaa gttgatcgcg
     7141 ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct gacctatgaa cagcgtctgt
     7201 tggcgattca acaactaggg cgtgattaca tggccgccgg gttatacgac cgcgcggaag
     7261 acatgttcaa tcagctgacc gatgaaactg acttccgcat tggcgctctg caacagttgc
     7321 tacaaatcta ccaggctacc agtgagtggc agaaagcaat tgatgttgcc gaacgcctgg
     7381 tgaagctggg taaagataaa cagcgcgtcg aaattgccca tttctactgt gagttagccc
     7441 tgcagcatat ggccagcgac gatctcgatc gtgccatgac cttgctaaaa aaaggggcgg
     7501 cggcagataa aaacagagcc cgcgtatcca tcatgatggg acgcgtgttt atggcgaaag
     7561 gagaatacgc caaagccgtc gaaagtttgc aacgcgtgat atcccaggac agagaactgg
     7621 tcagcgaaac gctggaaatg ttgcaaacct gctatcagca gttgggtaaa actgccgaat
     7681 gggcagaatt cctgcaacgc gcggtggaag agaacaccgg tgccgatgct gaattgatgc
     7741 ttgctgatat catcgaagcg cgcgacggta gtgaggccgc acaggtctat attacgcgcc
     7801 agcttcagcg tcatccgacc atgcgtgtgt tccataagtt aatggattac cacttaaatg
     7861 aagcggaaga agggcgtgcc aaagagagcc tgatggtgct gcgtgacatg gttggcgaaa
     7921 aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg ttttaccgca tacaccctct
     7981 actggcattg tccgtcttgt cgggcctggt caaccattaa accgattcgc ggtcttgatg
     8041 gcctgtaatt tttaaaaaaa tccgacttta gttacaacat gctagttatt gatgttccat
     8101 tgtgctccgg caacgacgga gcagaaaaag cctgccaggg gaaaaatcgc aactgttaat
     8161 tttttatttc cacgggtaga atgctcgccg tttacctgtt tcgcgccact tccggtgccc
     8221 atcatcaaga aggtctggtc atgacgttaa ctgcttcatc ttcttcccgc gctgttacga
     8281 attctcctgt ggttgttgcc cttgattatc ataatcgtga tgacgcgctg tcctttgtcg
     8341 acaagatcga cccacgcgat tgtcgtctga aggtcggcaa agagatgttt acattgtttg
     8401 ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga tatctttctt gacctgaaat
     8461 tccacgatat tcctaacact gcagcgcacg ctgtcgctgc tgcagctgac ttaggcgtgt
     8521 ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat gaccgcagcg cgtgaggcac
     8581 tggttccgtt tggcaaagat gcaccgcttt tgattgctgt aacagtgttg accagcatgg
     8641 aagctagcga cctggccgat cttggcgtga cactgtcacc tgcagattat gcagaacgtc
     8701 tggcggcact gacgcaaaaa tgtggccttg atggtgtggt gtgttctgct caggaagctg
     8761 tgcgctttaa acaggtattc ggtcaggagt tcaaactggt tacaccgggc attcgtccgc
     8821 aggggagtga tgctggtgac cagcgccgca ttatgacgcc agaacaggcg ttggcggctg
     8881 gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca gcgcaaacac
     8941 tgaaagcgat caacgcctct ttacagcgga gtgcatgatg agtgattcca acagccgtct
     9001 ggtctactca acggaaaccg gacgtattga tgagccaaaa gcggccccag tacgccctaa
     9061 aggcgatggt gtggtgcgta ttcagcgtca gaccagtgga cgtaaaggta agggcgtttg
     9121 cctgattacc ggtatcgatc tcgatgatgc cgaactgaca aaacttgcag cggaactgaa
     9181 gaaaaaatgc ggctgcggcg gagcagttaa agatggagtt attgaaatcc agggcgataa
     9241 gcgtgattta ttaaaatcac tacttgaagc gaaagggatg aaggtaaaac tcgcaggcgg
     9301 ttaacataaa aagccacgga tatatccgtg gctttcgaat attttactgt gtgtattatt
     9361 cagttatttc gtactgaaca aaatcaagcg tattataatt gcgagtcgat aatcgacatt
     9421 tatttacgat tatttaccga cctggtgacc aataacacca ccgacagctg caccacctaa
     9481 tgtacccaac gtactgccat cggtcagtac tgcaccgcct aatgcccctg cacccgcgcc
     9541 gattgcggtg ttgcggtccc gtttagacca gttagaacag gcactcagag acattgccaa
     9601 agtaattgcc agaacagccg cggtcatttt tttgctcgtt acaaacataa tactctctcc
     9661 tgaatttatg attcacggaa gtaagctctc tataactata atagctaaga ataagcctgg
     9721 tgaaattatt ccgtgaaatc tgctcgcgcg cagggttata tcacgcaggt gataatgact
     9781 tcctgttata tcgctgataa taattttata tcttaagcgt gttaataaca ggtaaatagt
     9841 cttaattatc aaccaggaat catcttagag cggatgattt gccaaactgc aaatcatccg
     9901 tagagaaggg aaatggttaa atgtcaacga cgtgaacggt gagtttactg tgctcaagat
     9961 gcatcagatc gctggcgcgt attttcgaat cggtaatcac gcgattgaat cgctcaacgg
    10021 gaccgatgga gtatgaatgt acagcaccaa atttcgagct gtcagtcagg actatcactt
    10081 cgcactcttt ttccagcacg gcattgacca catcggtacg catcatatcg cgaccggtga
    10141 atccagtttc aggttgccag ccatcaatac caataaatgc tttgctgaaa tgcacctgct
    10201 ggatgcactg gcgtgtcaac gggccaacca tactttcgct ttttttctgg tacacgccac
    10261 cgagcaaaat gacttcacaa ggcgcgtctt tcagcaaatg cgcgatgtag ctgctgaccg
    10321 tgatgatagt gacatttttc ttctgctccc ccagagtccg ggcgagcagg gcattgctgc
    10381 tgccattttc gataaagatg gtttcgcccg gttgaaccag tgacgcggca aactcggcga
    10441 gttcacgctt cagcgtatag ttgctcatca tacgggtttc gacgtcatca ctatcaagcg
    10501 aaactgcaaa gccatgtgca cggcggaggt aactcagttt ttcgagggtg ttgagatcct
    10561 ggcgaatggt aacttcagaa actccagtgg cttttgccag atcggttacg ctaacctgac
    10621 cctggtcaat gaccatctgt agaatagttt gttgtcggga attcatcgct gtaattctta
    10681 taacgttata atactaaata aaaaaatact gacgcgccgg gcatcctcaa ataggaaaac
    10741 ctgaaggcac gattgttaaa tctcccacgg tgccttcggg atgtctggcg tagattcagc
    10801 aacctggcgc aagagttctg ctttaagtat ctcgagatta tggatcgcag agtggtaatc
    10861 gcctgccaca aggatatcta acacaatatc aatacgttcg gcaacgcgct gggcatcaaa
    10921 ttcagacatc aaggacatcc ttatagcaaa agtggaaaat gaataatgca aaaattgccg
    10981 ctaaaagaga agtgtttaac agcaacggct aattatcatc caggaatacg atatataatg
    11041 acgggatata gcgctaagta tatatattca tctacttatg cgcgcttcag atagcgttta
    11101 taccagcgtt cgaaggcgac ggcgggcatc ggtttggcaa acaaaaatcc ttgccgctca
    11161 ttgatcccgt tcttggttaa aaaagcatct tcctttgcac tctctacacc ttcggcgatc
    11221 acctgaagat tcaatgcctg ggccacagcg acgatcgccc ggaccagtga ctgcgagaca
    11281 ggttgtttgt gaatatctcg aacaaaaacc tggtcaagtt tgatggcatc gatcggaaag
    11341 cgcgccagtt gcgaaagtga agagtagccg gtaccaaaat catccagatg cacttgcgca
    11401 cctagttggc taaattgttg aataacagac agtgccagtt catcattctc aatcagacaa
    11461 ctctctgtca gttcaacatc tatagggcag tattcaaaat tgagttcctg gagaacctgt
    11521 ttcagggcgg tgaaaatggt ttgatcggcg agctgacgtg cagaaatatt taccgccact
    11581 cgcaggttta tgcctttatc ccgccacttt gccacctggc gtacgacatc gagaatcacc
    11641 caacggccta aaggcacaat tagccctgac tcttcggcgt aggaaatgaa gtccaacggt
    11701 ggaatcaacc cacgttcagg tgactgccaa cgtactagtg cttccagact gcgcacttcg
    11761 ccacgccagg tgattttcgg ttgatagtga ataaccaact gatcgttttc cagtgcttta
    11821 cgcaagttgg tatccagcca gagatattca aatacccgtt gattcatttc tggggtaaaa
    11881 acgcaaaatt gtcctcgtcc gccttccttc gctgtgtaca ttgctgtgtc ggcgtgacga
    11941 ataatagccg tgctgtctga accatgttcg ggagagagtg caatacctac tgaacagctg
    12001 gtataaactt caattaaacc aatgcgaaag gggagccgta agcgggtcaa aattcgtgat
    12061 gccattgctt ccagcgcgct ttgtgaggtg ttggatgcca gtaccagaaa ctcatcccca
    12121 cctggacgcg ccaacacctg gtcatgttcg agacagctta aaatagccaa tgacacgtcg
    12181 cgtaataact ggtcaccaaa caaatgccca taggcgtcgt tgaccttttt gaaattatcc
    12241 aaatcaagat acacaacccc aactttattg ttatctgcat gattaatagc gtgatcgatt
    12301 aaatcctgca ttgcgttacg attcggcagt ccggtgatac tgtcggtatt tgccagaata
    12361 cgcagtcgct cctgagcgcg gcgctcttcg gtaatgtcgg tgccggaaca gattaaaaaa
    12421 atctcgcttt tgccactgcc gctgtggaca aatttattgc gaaacagaaa cagccgctgg
    12481 cctttacatg ttggtatcca cagttcgact tcatatgcat tgccgcttcg aaaaaataca
    12541 cggttattgc gcctggatgc cgcagcttca cgacggctca taaacagttt aaacacgctt
    12601 tggccaatga cgtcgtgttc tttcaaccca gtgtaatctt cacataaccg attgaagcgt
    12661 tggatattcc cccggctgtc gagaatcact attgcggaat gtgcttcaga aactacctgc
    12721 tcagcaaagg aaagcccgtg tgataaatca cgagcaactg ccggtgtgtc atgccatgca
    12781 gacgcgttgc cagcccattc ccgtttattg atttttcgtc ctaccagatg cacagaaaga
    12841 tcgctctcat cgacggtcag actcatcatc aggctggagg tgatgaccgt catttccctg
    12901 atgcgagcag cctgttcggc agataactgc aaagtacgat caggttctgt ggtttcggtg
    12961 gtagaaaagc gcaaaacatc gctgctttcc gtcaaccgcc agtatggatt gtgcgatccg
    13021 agaaagttgt acaacgttgt ggactcccta acggttttca tgatgcatat cctccggtta
    13081 aataaaa
//
LOCUS       DAJTOR010000114        12792 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTOR010000114 DAJTOR010000000
VERSION     DAJTOR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.114"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..243)
                     /locus_tag="OO249_002865"
     CDS             complement(<1..243)
                     /locus_tag="OO249_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5637095.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRI"
     gene            complement(313..1260)
                     /locus_tag="OO249_002866"
                     /pseudo
     CDS             complement(313..1260)
                     /locus_tag="OO249_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            1608..2480
                     /gene="rluF"
                     /locus_tag="OO249_002867"
     CDS             1608..2480
                     /gene="rluF"
                     /locus_tag="OO249_002867"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5637096.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2613..2885)
                     /locus_tag="OO249_002868"
     CDS             complement(2613..2885)
                     /locus_tag="OO249_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5637097.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(3137..4486)
                     /gene="lysC"
                     /locus_tag="OO249_002869"
     CDS             complement(3137..4486)
                     /gene="lysC"
                     /locus_tag="OO249_002869"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5637098.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            5011..6660
                     /gene="pgi"
                     /locus_tag="OO249_002870"
     CDS             5011..6660
                     /gene="pgi"
                     /locus_tag="OO249_002870"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5637099.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            7122..7364
                     /locus_tag="OO249_002871"
     CDS             7122..7364
                     /locus_tag="OO249_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637100.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            7478..8116
                     /locus_tag="OO249_002872"
     CDS             7478..8116
                     /locus_tag="OO249_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5637101.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            8113..8850
                     /locus_tag="OO249_002873"
                     /pseudo
     CDS             8113..8850
                     /locus_tag="OO249_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            8850..10946
                     /locus_tag="OO249_002874"
     CDS             8850..10946
                     /locus_tag="OO249_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5637102.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIGGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNVGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(10993..11271)
                     /locus_tag="OO249_002875"
     CDS             complement(10993..11271)
                     /locus_tag="OO249_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637103.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            11541..11939
                     /gene="psiE"
                     /locus_tag="OO249_002876"
     CDS             11541..11939
                     /gene="psiE"
                     /locus_tag="OO249_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5637104.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(11983..>12792)
                     /locus_tag="OO249_002877"
     CDS             complement(11983..>12792)
                     /locus_tag="OO249_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR5637105.1"
                     /translation="SPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKT
                     GGRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVI
                     NLTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAA
                     FAMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFH
                     NGFSYWIYGCMGVLAALFMWKFAPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         3125 a         3284 c         3311 g         3072 t
ORIGIN      
        1 gatgcgacca aaacgctgga taatttccgc taccgtatga tttacctctt tggcgctgga
       61 aatatcggtc ggccaaaact gatagccttt atgaccttca tattgaccat cgccaccgtg
      121 aatatctacc atctgtacgt ttgcgccttg tgctaataat tcctctacaa tcgccagacc
      181 aataccggat gcgccgccgg tgacaataat gattttatcc tgcaaattta accacgtttg
      241 cataacagac tcctgatttt agataatagg gccccgccgt aatttttatt acgctaaaca
      301 tcgggaagat ttttatttca gtaatagttc agctgtgctg ctattcgtta tcagacaatt
      361 aatatatttt ccacgcagtg caccaacaat tccgctgtat ttttcttcac tcatggcaat
      421 gccaatggaa tatcgtgcct gctttaattt attcatttcg atagagagtg ttttttcgct
      481 catattcgtt tcaaccattg cgccgtgaat atcaaaaaag cgcgagcaaa tatcgtcagc
      541 aacctggcgg gcattcaggt cgtcactctc ttcaccacca taaaacgcat gccagttagc
      601 gccgtcgcga atggccggtg agccaattcc caccagggcg acatccagat tatctcagta
      661 ggcagagatg gttttaaagt gctgagactg catgatccca ttacgaatta atgggttatc
      721 cagcagagcc ggaaaatccg cgagatgcga ttcccctttc agcttcgctg ccgcgctgta
      781 ggttaatgtg tttacatgat agcggctttc gagtttaccg gacggtccgc caataattgg
      841 tacgcagatt aactgccgcg attgccccgc ctgcggcaag ttttcaacca gtgcgctcac
      901 cgcgcgaccc caggaaaaac cgacaatatc gccaggctcc agcaagcgat ccagcagttg
      961 cgcgccgtgt aaccccatca tcgccagttg agtctcttca tcctcatcat ttcccgacac
     1021 caccacaacg tctttcaggc caaacttttg cttcagttgc tgctccagcc agagattttc
     1081 gttgtagtca tagttgatgg cgatggtgac aattccctga tcgcggcctc gtttaagcaa
     1141 gcggctgatg gtggtgcggt aaatacccag ttcgcgcgcg atttgcgcct gcgtcatatc
     1201 ctgttcgtaa tagagttggg caatcttcac aatcaaacgg atatcgtcac tgttttccat
     1261 tgccgcctcc tggttctgca catttgtgca aatatgcttt ctcaccgctt gatctgatat
     1321 aaccccatca tgagggcctt tcaaagcgat ttttcactct ttgcatgggg agatatagcg
     1381 ctttctcttg cacatttgtt tctctgcgta gtagtcatca gcacaggtgt gcagcgattc
     1441 gtaatatgtt gattatcctt attttgccag gcaaatttgc atcgtacttc cggagacact
     1501 tttgtgataa aaggcgtggc agatcacatt taatttcgac gttgccgcat aaccctgttt
     1561 ttccccgcta agaggtacgg tatattccgc gttttacagg agaatttatg ctgcccgact
     1621 catcagtccg tttaaataaa tacatcagcg aaagcggaat ttgctcacgc cgcgaagcgg
     1681 atcgctatat cgagcaaggc aatgtgttcc ttaatggcaa gcgagccacc attggcgatc
     1741 aggtgaaacc cggcgacatt gtgaaagtaa acggtcagtt gattgagcct cgggaagccg
     1801 aagatttggt acttatcgcc ctgaacaagc ccgtcggtat tgtcagcacc accgaagatg
     1861 gcgagcgcga taacattgtc gatttcgtta accacagcaa acgcgtgttc ccgattggcc
     1921 gcctggataa agactcccag gggctgattt tcctcaccaa tcacggcgat ctggtgaata
     1981 agatcctccg tgcgggtaac gatcatgaga aagagtatct ggtgacggtc gataaaccga
     2041 ttaccgatga atttattcgt ggtatggggg cgggggtgcc gatcctcggg acggtgacga
     2101 aaaagtgcaa agtcaaaaaa gaagcgccgt ttgtcttccg cattaccctg gtgcaggggc
     2161 tgaaccgtca gatccggcgc atgtgcgagc atttcggcta tgaagtgaaa aagctggaac
     2221 gtacgcgcat tatgaacgtc agcttaagcg gcattccgct gggggagtgg cgcgatttaa
     2281 ccgacgatga gttaatcgac ctctttaagc tcatcgaaaa ttcctcttcc gaggcaaaac
     2341 ctaaagcgaa ggccaaaccg aaaacagcgg gcatcaaacg tccagtcgtt aagatggaaa
     2401 aaacggcgga aaaaggcggt cgcccggcgt ccaacggtaa gcgttttacc tcgccggggc
     2461 gtaaaaagaa gggacgctga ttaacgcctc agactgatga aaacgtccaa aaagagctcg
     2521 gacagtcccc tcgccccttc ggggagaggg ttagggtgag gggaagattg tgtccacaca
     2581 gacgagtgaa aatctcaggc gttgattgtt cattagcgcc tgccgcgcgt cgacgtattt
     2641 gctgaccagc taaatgacgc ttcaggatcc ggtttataag cctgcttttt cttcaattgg
     2701 cgggcttttt tcgcttcagc ctcacgctcg accattaatt tatcgatgta ctctttctta
     2761 atgctgttgg tttcggagtt ggtcagtacg cgaccatgcg caatccgcgc ccgatccagc
     2821 agtgttttaa gttcacgctg ctcgcgttca gtcatctctt tttgggtaat acgggggagt
     2881 gccatgatgg tgccctcagt gagccagaga gttcagtgta cgggaattcg tttgcgagca
     2941 gaactgataa tattcatttt gccttctctt aactatttat ctctttcgat aattaattag
     3001 agatgcagat aaaaaaatta aaggcaatta ttctcgataa agacgttggt taacagagta
     3061 caggctcgtt tattgataag catagtgaca agaaaatcaa tacggcccga aatatagctt
     3121 ccaggccata cagtatttac tcaaacaaat tactatgcag tttttgcacc acctgctcgg
     3181 catcttcgcc gggcaccagg aagcacaggt tatggctgga tgcgccgtaa caaatcatgc
     3241 gaatgttgaa cggttccagt acgccgaata cctctttgcc aacgccgcag gcttttgaca
     3301 ggtcattgcc aatcaacgcg accagcgcca gaccttcttc tacttccacc cggcacagtg
     3361 cggaaagctc catcagcaaa gattgcgtca gcaacgtatc gccagtggag gttgaaccgg
     3421 tggtatcaag ggttaatgcc acgctcactt ctgacgtggt gattaagtct accgaaatat
     3481 tatgccgcgc gaggatgccg aaaacttccg cgaggaagcc gcgagaatgc agcatattca
     3541 ggctgtgcaa agtgagcaga gtctgattgc gacgaagcgc cagcgcgcgg aacagcggag
     3601 gattttcagt tttattgcac accagcgtac cacctgcgcg tgggtctttg ctggagccga
     3661 caaagaccgg gatatcgctg cgtactgcgg gtagcaacgt tgccggatgc agtacttttg
     3721 caccaaaagt tgccatctct gccgcttcgg caaacgcgat ttcatcaatg cgttttgctg
     3781 cggaaactac gcgtggatcg gtggtgtaga tgcccgggac gtcggtccag atatcaacac
     3841 gagatgcgtg taaagcctcc gccagcaagg ctgccgtata atcgctgcct ccacggccaa
     3901 gcgtcgttgt acgaccttta ttttcgctac cgataaatcc ctgggtgatc actaagccgt
     3961 cattgaggcg tgggagcagc tgcagcgcgg ccagttccgc cagcgcggct acatctggct
     4021 ctgcacgacc aaatcggtcg ttggtacgca tcactttacg tacatcaaac cactgtgcct
     4081 gaacatcgcg ttcgcgcagg atttcgacaa acagcagggt cgacatcagc tcgccgtggc
     4141 tgaccagctc atctgtcagc gccggagacg ttgccagcgc cgccgcttct gccagaacag
     4201 taatgttctc cagcagacgt tcaatctctt cacggataac gttcgggtaa cgcagacgtt
     4261 ccagaatggc aaactggatg ttgcgaatag cgtcgagttt ttcgaatcgc tcgccaggtt
     4321 ccagcccttc cgctaaagcg accagcagat tagtgatacc agcagaagcc gagaggacta
     4381 ctaaacgcac gttggcatca gaaagcacaa tatcagcgct gcggttcatg gcgtcaaaat
     4441 cagctacgct ggtaccgcca aatttggaga caacaatttc agacataact acctcgtgtc
     4501 aggggatcca ttttcagcct tggcacaagg gaagagcgga agacgggtgg gcgcagagcg
     4561 atacttcgct actattttca cccagaagtg ctccaccact tgcgaaacgc ccgactgcga
     4621 acgcttctgg tgacaaccca ggggattcag cccctgtagc cgatgatgaa cgtggccagc
     4681 cgttcaatca cctcggcgat gcaccccctc aggtgttatc acaggactgg ctcctccaac
     4741 accgttactt gggcaacgcg cctcttctgg cctgcgctag cgcaggtagt acatttataa
     4801 ataaagggtg agcggggcgg ttgtcaacga cggggtcatg cggatttttc atccactcct
     4861 ggcggtcagt agttcagcta ataaatgctt cactgcgcta agggtttaca ctcaacatta
     4921 cgctaacggc actaaaacca tcacattttt ctgtgactgg cgctacaatc ttccaaagtc
     4981 acaattctca aaatcagaag agtattgcta atgaaaaaca tcaatccaac gcagaccgct
     5041 gcctggcagg cactacagaa acacttcgat gaaatgaaag acgttacgat cgccgatctt
     5101 tttgctaaag acggcgatcg tttttctaag ttctccgcaa cattcgacga tcagatgctg
     5161 gtggattact ccaaaaaccg catcactgaa gagacgctgg cgaaattaca ggatctggcg
     5221 aaagagtgcg atctggcggg cgcgattaag tcgatgttct ctggcgagaa gatcaaccgc
     5281 actgaaaacc gcgccgtgct gcacgtagcg ctgcgtaacc gtagcaatac cccgattctg
     5341 gttgatggca aagacgtaat gccggaagtc aacgcggtac tggagaagat gaaaaccttc
     5401 tcggaagcga ttatctccgg tgagtggaag ggttataccg gcaaagcaat cactgacgta
     5461 gtgaacatcg ggatcggtgg ttctgacctc ggcccgtaca tggtgaccga agcactgcgt
     5521 ccgtacaaaa atcacctgaa catgcacttt gtttctaacg tcgatgggac tcacatcgct
     5581 gaagtgctga aaaaagtaaa cccggaaacc acgctgttct tggtagcatc taaaaccttc
     5641 accactcagg aaactatgac caacgcccat agcgcgcgtg actggttcct gaaagcggca
     5701 ggtgatgaaa aacacgttgc aaaacacttt gcggcgcttt ccaccaatgc caaagccgtt
     5761 ggcgagtttg gtattgatac tgccaacatg ttcgagttct gggactgggt cggcggccgt
     5821 tactctttgt ggtcagcgat tggcctgtcg attgttctct ccatcggctt tgataacttc
     5881 gttgaactgc tttctggcgc acacgcgatg gacaagcatt tctccaccac gcctgccgag
     5941 aaaaacctgc ctgtcctgct ggcgctgatt ggcatctggt acaacaattt ctttggtgcg
     6001 gaaactgaag cgattctgcc gtatgaccag tatatgcacc gtttcgcggc gtacttccag
     6061 cagggcaata tggagtccaa cggtaagtat gttgaccgta acggtaacgt tgtggattac
     6121 cagactggcc cgattatctg gggtgaacca ggcactaacg gtcagcacgc gttctaccag
     6181 ctgatccacc agggaaccaa aatggtaccg tgcgatttca tcgccccggc tatcacccat
     6241 aacccgctct ctgatcacca ccagaaactg ctgtctaact tcttcgccca gaccgaagcg
     6301 ctggcgtttg gtaaatcccg cgaagtggtt gagcaggaat atcgcgatca gggtaaagat
     6361 ccggcaacgc ttgactacgt ggtgccgttc aaagtgttcg aaggtaaccg cccgaccaac
     6421 tccatcctgc tgcgcgaaat cactccgttc agcctggggg cgttgattgc gctgtatgag
     6481 cacaaaatct ttactcaggg cgtgatcctg aacatcttta ccttcgacca gtggggcgtg
     6541 gagttgggta aacaactggc gaaccgtatt ctgccagagc tgaaagatga caaagaaatc
     6601 agcagccacg atagctcgac caatggtctg attaaccgct ataaagcgtg gcgcggttaa
     6661 tcatcatgcg tgcctgatgc cgatatgtag gccggataag gcgtttacgc cgcatccggc
     6721 aaccggagcc tgatgccaat atcgacatta atgcatagta tgaggagtta aataacctta
     6781 aaaacaatgc cgacaataag tcggcattgt tttatcagat aaatcccctt gtctgtgatt
     6841 taacggaaat cataccgtga ggttaatcct aaaatagatt tttaatcgtt gtttatttcg
     6901 gagaatacgc agattaatta cttttgtttt tattttaagt ttatgatttt tattgttatt
     6961 taaatataag ttgaagctta tatttgatat tcattccaat tatcctaaaa cgccatcgct
     7021 aattccccgc gccgtaattc gcatgcttta gttgtgtata ctcgatcccg cccgaaatgt
     7081 ttttgggtaa atctccattc attcaatgaa gggaaattgt tatgaaaaaa gttctgtatg
     7141 gcatttttgc catatctgcg cttgcggcga cttctgcgtg ggctgcacct gtacaggtgg
     7201 gcgaagcggc agggtcggca gcaacgtcgg tttcggcggg tagttcctcc gcgaccagcg
     7261 tcagcaccgt aagctcggcg gtgggtgtcg cgctcgcggc aaccggtggc ggtgatggtt
     7321 ctaataccgg gaccaccaca accacgacca ccagtaccca gtaataaagt atgtatcccc
     7381 aaaataattt gagtcattgc atctgtggct tgaagtatga agggatttac cataaccaca
     7441 ctccggtgtg gttattctgc ccctctggag aagagtcgtg aagcgacctg cactcattct
     7501 tatctgcctg ctattacagg cctgttcagc cacgactaaa gagctgggca attcactgtg
     7561 ggacagtctg ttcggcacgc caggcgtaca gctgacggac gatgatattc aaaatatgcc
     7621 ctacgccagc cagtacatgc agcttaatgg cgggccgcaa ttgtttgtgg tgctggcctt
     7681 cgctgaagac ggacaacaaa aatgggtcac tcaggatcaa gccactcttg tcacacaaca
     7741 tggtcgtctg gtgaagactt tgcttggcgg cgacaacctg attgaagtga ataaccttgc
     7801 cgccgacccg ctgattaaac ccgcacaaat tgttgatggt gcaacatgga cgcgcacgat
     7861 gggctggacc gagtaccagc aggtacgcta cgccaccgca cgctcagtct tcaaatggga
     7921 tggcaccgat accgtcaaag tcggcagcga tgaaaccttg gttcgcgtgc tggacgaaga
     7981 agtctccacc gaccaggcgc gctggcataa ccgctactgg atcgacagcg aagggcaaat
     8041 tcgccagtcg gaacagtatc tcggcgcgga ttatttcccg gtgaaaacca ctctcatcaa
     8101 ggcggcaaaa caatgattaa acaaactttt gtcgcgttgc ttttgagtgt gggagcgtca
     8161 tcggtcttcg cggcaggaac cgtcaaggtg ttcagcaatg gcagcagtga ggccaaaaca
     8221 ctaacaggcg cagagcattt aatcgatctg gtaggctaac cgcggctggc aaacaactgg
     8281 tggcccggtg cggtgattag cgaagagctg gcaacggcgg cagcattgcg tcagcagcag
     8341 gcgttgctga cacggctggc tgaacaggcc gcagactcca gcgccgacga tgccgccgcc
     8401 attaacgcct tacgccagca aattcaggcg ttgaaagtga cgggcaggca aaaaatcaat
     8461 cttgatcccg atatcgtgcg cgttgccgaa cgcggtaacc caccgttgca gggcaactac
     8521 acgctgtggg tcggaccacc gccgtccacg gtcacgttgt tcgggcttat cagccgtcct
     8581 ggcaagcagc cattcactcc aggtcgcgac gtggcgagct atctctccgg gcagaacctg
     8641 cttagcggtg cggatcgcag ctacgcgtgg gtggtttacc cggacggacg cacgcaaaaa
     8701 gcgccggtgg cttactggaa caagcgtcac gtagagccga tgcccggcag cattatttat
     8761 gttggcctcg cggactccgt ctggagtgag acgcctgatg cccttaacgc cgacattctt
     8821 cagactctga cgcagcggat accccaataa tgaaaaaaag acatctgctt agcttactgg
     8881 cgctgggcat tagcatggct tgctacggcg aaacatatcc tgcgcccatt ggcccgtcgc
     8941 agtcggattt cggtggcgta ggattattac aaacgcccac cgcacgcatg gcgcgggaag
     9001 gggagttgag cctgaactat cgcgataacg atcagtaccg ttattactca gcttcagtgc
     9061 aactcttccc gtggctggaa acaacgctgc gctacaccga cgtgcgcacc cggcagtaca
     9121 gcagcgtcga agcgttctct ggcgatcaaa cgtataaaga taaagccttc gatctcaaac
     9181 tgcgtttgtg ggaagagagt tactggctgc cgcaagtggc ggttggtgcg agggatatcg
     9241 gcggtacggg gctgtttgat gcggaatatc ttgttgccag caaagcctgg gggccgttcg
     9301 attttacgct cggcctgggc tggggttatc tgggcaccag cggtaatgtg aaaaatccgc
     9361 tctgttcagc cagtgataaa tattgctatc gcgataacag ctacaaacag gcgggatcta
     9421 tcggcggtag ccagatgttc cacggtcctg cctcactgtt tggcggcgtg gaataccaga
     9481 cgccctggca accgctgcgc ctgaaactgg agtatgaagg caataattat cagcaggatt
     9541 ttgccgggaa gctggagcaa aaaagtaagt ttaacgtcgg tgcgatttat cgcgttaccg
     9601 attgggccga cgttaacctt agctatgaac gtggcaacac ctttatgttt ggcgtcacgc
     9661 tgcgcaccaa ctttaacgat ctgcgcccgt cttacaacga taacgcccgc ccgcaatatc
     9721 aaccgcagcc gcaggatgcc attttgcagc attcggtggt ggcgaatcag ttaacgctgt
     9781 tgaaatacaa tgtcggactt gccgatccgc agatccaggc gaaaggcgat acgctgtatg
     9841 tcaccggcga gcaggtgaaa tatcgtgatt cgcgcgaagg gatcatccgt gctaatcgga
     9901 tcgtgatgaa cgatctgccg gatgggatca aaacgatccg cattacggaa aatcgtctta
     9961 acatgccgca ggtgacgacg gaaaccgatg tcgccagcct gaaaaatcat ctcgccggag
    10021 agccgttggg ccacgaaacg aagctggcgc aaaaacgcgt cgagccagtg gttccgcagt
    10081 ccaccgagca gggctggtat atcgacaaat cacgctttga tttccatatc gatccggtgc
    10141 tgaaccagtc ggtcggtggc ccggaaaact tttacatgta tcagctgggc gtgatgggaa
    10201 tggcagattt gtggctgacg gaccatctgc tgaccaccgg cagcctgttt gcaaatcttg
    10261 ccaacaacta cgacaagttt aactacacca atccgccgca ggactcgcac ttaccgcgcg
    10321 ttcgtaccca tgtgcgcgag tatgtgcaga acgatgtcta tgtgaataac ctgcaagcca
    10381 actacttcca gcatctgggc aacggcttct acggtcaggt ctatggaggt tatctcgaaa
    10441 ccatgtttgg cggtgcgggg gccgaagtgt tgtatcgtcc gctggacagt aactgggcgt
    10501 ttggtctgga tgccaactac gttaaacagc gcgactggcg tagtgcaaaa gatatgatga
    10561 aattcaccga ctacagcgtg aaaaccggac atctgaccgc ctactggacg ccatctttcg
    10621 cccaggacgt gttggtgaaa gccagcgtcg ggcagtatct ggcaggggat aaaggcggca
    10681 cgctggagat cgccaaacgc tttgacagcg gcgtggtggt gggtggctat gccacgatca
    10741 ccaacgtttt gaaagaggag tacggcgaag gggacttcac caaaggcgtg tatgtctcgg
    10801 taccgttgga tctcttctcg tctggcccga cacgcagccg tgcggcgatt ggctggacgc
    10861 cgctgacgcg tgacggtggt cagcaacttg ggcgtaagtt ccagttgtat gacatgacca
    10921 gcgaccgtag cgtcaatttc cgctaagtca tgggaaaggt gccagttttc gcactcactg
    10981 gcaccttcat tcttaatggc ctgtcgagcc atcaggaacg cagttcaccc ccggtttgcg
    11041 atctggtgtg taaaggcgaa aacattctgt cttcctgtcc gtcgatatcg gttgcaggtc
    11101 tggttgcctg agcgactggg ccatcattgg gctggagaca ttgctttgcg gtaggtcggc
    11161 tttatcagca gtaccgagcg gaccagcata agcaggaaga acagagactg ataacatcaa
    11221 agcagcaaaa taaggcttca tttttaccac ctttatcagg ttacgtttca tttgttccag
    11281 aggaacattg tcgatttttc gcgcattgct ggtggctggg aatcacctga atgggtgatt
    11341 tttgaattac cggcttgggt gcggtttgtc ttgccggatg cgccgccagg cgcggcttat
    11401 ccggcctacg ggtaggtata tccggctttg gtggaggcgc gctccaaatc caggttgaac
    11461 aaaacataca caaaaaatat agatctccgt cacatttttg cgttatacag gaagctcgcc
    11521 actgtgaagg aggtactgct atgacgtcac tctctcgtcc gcgcgtggag tttatctcca
    11581 ccattttgca gaccgtactc aatcttggcc tgctgtgcct cggcctgatt ttggttgtct
    11641 tcctcggcaa agaaacggtg catctggcag atgtgctgtt cgcgccagaa caaaccagca
    11701 aatatgagct ggtggtttac tttctctatt tcgaatttat cgcgctgatt gtgaagtact
    11761 ttcagtccgg ttttcacttc ccgctacgtt actttgtcta tattgggatc accgccattg
    11821 tgcgcttgat catcgtcgat cataaatcgc cactggatgt gttgatctac tcggctgcga
    11881 tcctgttact ggtgatcact ctgtggctgt gtaattcgaa acggctgaag cgggagtaaa
    11941 aagacagcac gccgaactgg cgcggcgtgc tggacaggaa gattacagcg tagcagtttg
    12001 ttgtgttttc ttcgtttccg gttcccagag ggcttccagc tcctcaaggg ttttaccttt
    12061 ggtttccggg gcaaatttcc acataaatag tgctgccaga acgcccatac acccgtaaat
    12121 ccagtaggag aaaccgttgt ggaaatgggc caccagccag gagtttttgt ccatcatcgg
    12181 gaaggtccag gagacgaagt agttcgccag ccactgggcc gccaccgcga ttgccagcgc
    12241 cttaccacga atagcattcg ggaagatttc cgacagcagt acccagcata ccggacccca
    12301 ggacatggca aaggcagcaa catagaacag catcgacagt agcgccacaa tacccggtgc
    12361 ctgagtgtaa aacgcggtac cgaggctaaa cataccgatt gccattccga gtgcgccgat
    12421 aatttgcagt ggcttacgac caaatttatc caccgtcata attgccagaa cggtgaaggt
    12481 gaggttgata actccgacaa taatggtctg caacagcgcg atatccgtgc tggcccccag
    12541 cgttttgaac acttccggcg cgtagtacag caccacattg atgccgacaa attgctggaa
    12601 gatggagagc ataacgccga tgacaatcac gcccacgcca aacatcagca gacgaccacc
    12661 ggttttgcgg ccatgatcca gggagtgttt aatttcctgt actgcctgag ttgcaagcgt
    12721 gttgcccata attttgcgca ggatactttc cgcctgttct tgcttgccgc gtgacatcag
    12781 ccagcgagga ct
//
LOCUS       DAJTOR010000115        12784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTOR010000115 DAJTOR010000000
VERSION     DAJTOR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.115"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(89..775)
                     /locus_tag="OO249_002878"
     CDS             complement(89..775)
                     /locus_tag="OO249_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5637106.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1356..2144)
                     /gene="phoH"
                     /locus_tag="OO249_002879"
     CDS             complement(1356..2144)
                     /gene="phoH"
                     /locus_tag="OO249_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5637107.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2766..4037)
                     /gene="efeB"
                     /locus_tag="OO249_002880"
     CDS             complement(2766..4037)
                     /gene="efeB"
                     /locus_tag="OO249_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5637108.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(4043..5170)
                     /gene="efeO"
                     /locus_tag="OO249_002881"
     CDS             complement(4043..5170)
                     /gene="efeO"
                     /locus_tag="OO249_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5637109.1"
                     /translation="MTINFCRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITAQAEDLAQLRGVLGLD"
     gene            complement(5228..6056)
                     /locus_tag="OO249_002882"
                     /pseudo
     CDS             complement(5228..6056)
                     /locus_tag="OO249_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            complement(6725..8233)
                     /gene="putP"
                     /locus_tag="OO249_002883"
     CDS             complement(6725..8233)
                     /gene="putP"
                     /locus_tag="OO249_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR5637110.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(8392..8601)
                     /locus_tag="OO249_002884"
     CDS             complement(8392..8601)
                     /locus_tag="OO249_002884"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637111.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            8656..12618
                     /gene="putA"
                     /locus_tag="OO249_002885"
     CDS             8656..12618
                     /gene="putA"
                     /locus_tag="OO249_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5637112.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNCNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTYETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(12658..>12784)
                     /locus_tag="OO249_002886"
     CDS             complement(12658..>12784)
                     /locus_tag="OO249_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HCR5637113.1"
                     /translation="HYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         3165 a         3475 c         3175 g         2969 t
ORIGIN      
        1 tttaaagaag agctcgtacg aaggtacggg ctttttttcg tatattgcac acaccggggg
       61 gatgagaagc cccgaccggg atacttagtt aatgcaccgg ttcactcttt gtcgccgcga
      121 ctaatgggcg aattttgtca cgagtctccc agaccggagg aatttcctcc aatttagtga
      181 cagaagctcg tacaaaaggc acccgtgcaa gagtcataat tgtatccaac caacggtcac
      241 gaataatgcg atccgccctt tcatgactcg ggtcatccag ctcaataaca cagactattt
      301 tattgttctt ctttgaaacc agaacataat ccattgccat gccattataa attgcaaaga
      361 aacgctttct ttcttcttcg ctaaactcag gttgttctgg aaagttgatc aagcgcccca
      421 gagagacctg gcaatggata tgacaataat gtccaaacca tttttccaga ttatcgaaat
      481 acattttttc acaacgagtg attaaccact cttttctttc caataaaata ccttcggcaa
      541 ccattccctg aaataactca tctcgctctt gccaacgaga gtcacgatag gttttctgcg
      601 gtatatagct ccttgtcgac gccttagcgg caggggaagt tgcagatgaa gggacgggtt
      661 gcgatttagc gatgattacc gatgaggcaa cttttcggag tgtcaatttt ctggcaacag
      721 caaaaccggc gataaatgca attgtcaaac aagttacaaa agccataaag ctcataatta
      781 attttcacct atccatgttg attatatttc attaccaatg ttactcgctg cagcgtgtta
      841 catgccaaca atgcaacgat caatatatag ttagtatcat gttttgatta ttacaaccat
      901 ccctatttag ccattgcggc ttgccttcca caaaaaaata acaacacaca atattagcaa
      961 agaattaatt aagattaacc ttacatttat aatattatcc ctaaagaata aatcagagga
     1021 atatattcgg tgttaaacag tcagtcaaag tatagcccat tgttgattaa acctcataag
     1081 caaaattggc accagatctc ctgtacaggt tgaatggccg aaaaaagata ggcagatgct
     1141 catttttttg gcttctggaa gaaaaactct ccgcgctggc agtattacac tggtctgatg
     1201 ctatgacgaa aaatgcctgg taagagtatc taaaccagac tccagttcag cttatcaaca
     1261 cgctcaaagt cgcccccctg aacgctaacc tgaagtgaaa atctgttgaa ataaaacaaa
     1321 gcccggttcg cccgggctct gcaccgataa cacacttaac tgtatgcatg cagcgtacgt
     1381 tggcaaagtg ccgaacgtac gcaatcctct tttccgaacc ggacgatccc aaccatttca
     1441 tcttcttcga aacgttccag cgcgtcactt aatccggagc acacgccgcg aggcaaatcg
     1501 cattgcgtga tatcaccgtt gacgataacc gtcacgttct ccccgaggcg ggttaaaaac
     1561 attttcattt gcgcggcagt cacattctgc gcctcgtcaa gaatgacgac tgcattttca
     1621 aaggtacgtc cacgcatata ggcgaacggc gcgatttcca ccttccctat ttccggtcgc
     1681 aggcagtact gcataaagga agcccctaag cgccggacca gcacgtcgta gaccgggcga
     1741 aaatagggag caaacttttc tgcgatatca ccaggtaaga agccaagatc ttcatcggct
     1801 tgcagaactg gacgggtgac gataatcctg tcgacatcct tatgtatcag ggcctctgcc
     1861 gcttttgctg cgctgatcca ggtttttccg cacccggctt cgcccgtggc gaatatcagc
     1921 tgcttactct caatagcctt cagatagtgc aattgcgctt catttcgcgc gaggatgggc
     1981 gaggtatcgc gactgtcgcg ggccatacca atggcttcta cgccgctcat ctgcacaagc
     2041 gaggtgaccg attcttcttc acgctgctta tggctgcgcg aatcccgtct cagcacacgt
     2101 tttgcctcgc gacgagcttt gatcactgct ttttgtcttc ccatggagag caccttgagt
     2161 tgtttgtatt catcacacgc gccgttggca gcgcgattat gcgcacgaac atcagagggt
     2221 tggcttcctt gtaagccata gtttgctttt ggataaaatg ccgaaaaacg gctacgcgca
     2281 ccgtttacgg cgtcggtaac acatgaaaag aaaggatgag gttgaaaatg caaagtgacg
     2341 agatgactac cggaggagaa aactccgcga gtggtggcgc gttgattatc taaaacatgt
     2401 ccagtacagg acgttaccat ccgcgatctc catagtgact gactatcact gccgggaact
     2461 tccgctgcta cttaataagt acaacagatc tcgcatttta ttgcaacaat atatttactt
     2521 atatttaact ataaaacact atttcagtga cattagtttc tactggaaag atgacagagt
     2581 gatgacagtg atgaaaaaag ctgtgtgctt tcagcaggat ttgcatcagg catcagtgca
     2641 cgatcgccgg atgcggcgtg aacgccttat ccagcctacc aaacccgcac tgattgtagg
     2701 catgataaga cgcgcaagcg tcgcatcagg catctgcgca caaatgcctt atccgcctaa
     2761 aaacattaaa cccgcaataa cgcgcttccg agataatcgt tcgcgtcctt cactcccggc
     2821 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     2881 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     2941 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     3001 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     3061 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     3121 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     3181 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     3241 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     3301 ttatcattct ggctatcggg attggcagtg ccgtctttaa agcccagcaa attaatcggc
     3361 gtctctttgc ctttactacg cgctgcgtga tcggaaataa acccttcccg cttccagcgc
     3421 acgctgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggat caccgtgtcc
     3481 tgggtgttgg cgcaaatttg tagcaacaca tcaccatggc ataacgccgc atccagcgag
     3541 tcgttgggga aacgcgtcat cttctgcaac tttttcggca tctgtggcgc aaggccaaac
     3601 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     3661 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgcc
     3721 gctccgccct gagtcagaaa agcaaaacgc tgagtcaaca agcgaaacaa ccgttcaaga
     3781 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     3841 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     3901 gaaagcgtac ccggcgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     3961 agcgccagcg cacctatcac tttcagtaaa cgtcggcgtg acggttcatt cacgccgttt
     4021 tcatctttat actgcataac gcttaatcca gtcccagcac accgcgaagt tgcgccagat
     4081 cttccgcctg cgcagtaatc ggtcctttca gtgcattccg gtcggcatcg gtcaatttgt
     4141 cgtaggtttc aaaaccgtct ttagtacggt atttcgccag aatggtatcg acctttttaa
     4201 agttggcatc gacttttgcc agcagttccg ggttggcttt ttgcagttgt ggacgcagca
     4261 aatcgacaat tttctgcgag ccttcaacgt tagcctggaa atcccacaga tcggtgtggc
     4321 tgtagcgatc ttcttcaccg ctaattttgc tggctgccac ttcctcaatc agtccggctg
     4381 cgccgccgac cacttttgaa ggtgggaaag ccagttcact gatgcgtttt tgcaaatcga
     4441 ccacatctgt ataaagctgc tcagcgtact gatccatccc tttggtggtg ttgtcgccaa
     4501 acaatgcttt ttccagacgg tggaaaccag tgaattttgg gtcggcggct ttttgctcgt
     4561 aatcatcttc acgggcgtca atgctgccat ccagatcgga gaacagttca gcaatcggtt
     4621 caatgcgctc atagtgctgg cgcgtcggcg catacagtgc tttcgctttt tcgatatcgc
     4681 cagctttaat cgcgtcggta aaggctttgg tgtcggtcac cagctgcgtg gtttccgcca
     4741 tgacatacgc tttatatgca gtaattgcac caccaagact taacagcgca tcactttgcg
     4801 ccgcatccgc cgttgcctca cctttgacga tcaacttccc tttcgggtta gtcagcagac
     4861 cgcaggtcat atcgtattcg ccaggctgta aattcgccgt cattttctgg ctaaaaccag
     4921 gggcgatatt ttcccgctct tccaccacca tcacgccttt gaggatctcc cactccagcg
     4981 ccttctggct atggttctga ataatgaact gtgttttccc ggcgttaacc gtaatggtca
     5041 tcggttcgca ctgcttatcc gtcacggtca ctttgacctg cggcacatca gcggcgttag
     5101 ccataaaagc agaagaaaac agcgcagcca cgctcaactg caatgcgtta cggcagaagt
     5161 taatggtcat gacgagacta tccctttaaa gtatgttgta actaagcaag agtttgcgtc
     5221 gtatttgtta cgcggagcga gacgctgtcg cccctgcgcg tggtggcaga gcaaatgcca
     5281 ccagcgccgg gatgagataa ataaaccaga cggcgacttc gctgacgctc ggcgcttcct
     5341 gatagccaaa aatcccttcc atcagcgtgc caaacagcga gtgagttgag agcaccgcac
     5401 tcatatcgaa ggcgatttcc tgaaagtggt tccacaatcc ggcttcatga aatgcgcgaa
     5461 tggcaccagc tgccagccct gcggcgacga agagaataaa caggctggtc catttaaaaa
     5521 atgcaccaag attgaggcga ataccgcccc agtagagcag aaagccgagc accacggctg
     5581 tagcaagacc gagcattgca cccagcggcg gccagatccc gacatcttgt tgaaatgccg
     5641 ccagcaggaa aaagaccgac tccagccctt cccttgcaac ggcaaaaaag accatcatca
     5701 ccagcgccca gccatgatga tttccacgct acaatgcgct atcgactgcc tgttccagtt
     5761 gcacttacgt tgcgcgacac tttgcgcatc cagaaaacca tccaggtaag gatcaccacg
     5821 gcgatcaccg ccacgatacc ttcaaacagt tcctgttctt tttgcggaaa ttcgccggtg
     5881 gtttcgttaa tgaagatccc caggcccagg cacaacgcag cggcaagcaa cacgccaatc
     5941 cacatcacac caatccatcg gcctcgctgg gtacgcttaa gatagctggc aatcaaactg
     6001 acaatcagcg cggcttcaag tccttcgcgc aacataatga gaaacggaac aaacatgcca
     6061 cccaccctta aacgtttttc tgagtcaatc tatgcaaaga aaagtaaatc gcagtgatag
     6121 cgattatcat tacggtaaag aaaaatacaa gcggaatgtt tataaaatga tgttggaatg
     6181 taaacaaaga gaggtaatgc caggggatat aaacgattat tccagggtag aaagatggga
     6241 ttgtttgcaa agaaccatgt gttcttgccg gatgccgcgt aaacgccata tccagccgac
     6301 aaaactgtcc agactcaatg catcagagct tccatgtaaa actgataagc gggtgtatca
     6361 agcaatttcg tccatccgcg attatcaaat ctaacttttt gcccggaaca taataacagc
     6421 tcgggtgttg gtggcgtcaa aatcgctgaa gcgttccccg aaggccggga cagcccacat
     6481 ggatgtgggc tgagggcgcg tcttacagga cgttacctcg cgcccgaccc ggtagccgaa
     6541 ggggataagc tgaaggtacc gcgcagcggc gattttgttc gccagagccc gggggtgcac
     6601 agggggcggc ggcgattggc cgccccctgc gcgctccctg caccattcaa tttatatggc
     6661 gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc gcaggctaag
     6721 tcccttagct ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca tcggcctcgg
     6781 caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac actacaatcc
     6841 cgatactgcc gaagatgaag cccggaataa tttcgtacag tcccagccag ccgaactgct
     6901 tccagacgat aaccgtcagc gcaccgatga tcatccccgc cagtgcaccg ttacgcgtca
     6961 tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca aagcctgccc
     7021 acgcgtagct cactaagccc agcacgcggt tttccgggtt tgccgccagc gcaatcgcca
     7081 ccagcgccac caccagcacc atcacacgcc ctacccacac cagctctttc tggctggcat
     7141 gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac accagcagtt
     7201 ggcaacttaa ggttgacatt accgccgcca gaattgccga cagcagaatc ccggcaatcc
     7261 acgggttaaa cagaatttgc gccagttcga taaacacgcg ctcggcgttc tggtttaccg
     7321 cgcctgctac cgccgggtgc tcattaaagt aagcaatccc aaagaagccg acagccaccg
     7381 cccctgccag gcagaggatc atccaggtca tactgatacg acgcgcatgg acaatgctgt
     7441 ggtgagaatc cgccgccata aaacgcgcca gaatgtgcgg ctgcccgaag taacccagcc
     7501 cccaacccat cagtgagata atggcaacaa agttcagacc tttgagcata tcaacgtttt
     7561 cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca ctgataatga
     7621 cgataaccgg tgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca gtgtcagtcc
     7681 agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgccgca gctcccgccc
     7741 acagagccgt ttcgtagctc atgccaaagg tactttcaaa cagacgcgcg cctgccacaa
     7801 tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag ataatgcgca
     7861 aaatgcggct tttatcttca aaacgcccgg tgaaataatc cggcaatgtt aaggcgttat
     7921 tattgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg atccacgcgc
     7981 ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa accgcgccag
     8041 gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat gccgtcacaa
     8101 atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt gatcgccagg
     8161 cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc aacatcggtg
     8221 tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa acttgccgtt
     8281 atatctgcaa ccggaacggg gtaacagagt ttatttttta ccagggcgac cgtatcctgc
     8341 cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat tttagtgctc
     8401 agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg cacctttcaa
     8461 aaatgttaac tgccgcagag aaaaagtctg agttattttt ttaatccctg tcatatcaat
     8521 ttcttttatt aacatttcat tcatttttaa gcttgctacg caggtcacat ttaacatggt
     8581 tgcacaaagt tgcaacatca tggatatttc acgataacgt taagttgcac ctttcagaac
     8641 aacaggagta atggcatggg aaccaccacc atgggggtta agctggacga cgcgacgcgt
     8701 gagcgtatta agtctgccgc gacacgtatc gatcgcacgc cacactggtt aattaagcag
     8761 gcgatttttt cttatctcga acaactggaa aacagcgata ctctgccaga gctacctgcg
     8821 ctgctttctg gtgctgccaa tgagagcgat gaagcaccga ctccggcaga ggaaccacac
     8881 cagccattcc tcgactttgc cgagcaaatt ttgccccagt cggtttcccg cgccgcgatc
     8941 accgcggcct atcgtcgccc ggaaaccgaa gcggtttcta tgctgctgga acaagcccgc
     9001 ctgccgcagc cagttgctga acaggcgcat aaactggcat atcaactggc cgataaactg
     9061 cgtaatcaaa aaaatgccag tggtcgcgca ggtatggtcc aagggttatt gcaggagttt
     9121 tcgctgtcat cgcaggaagg cgtggcgctg atgtgtctgg cggaagcgtt gttgcgtatt
     9181 cccgacaaag ccacccgcga cgcgttaatt cgcgacaaaa tcagcaactg taactggcag
     9241 tcacacattg gtcgtagccc gtcactgttt gttaatgccg ctacctgggg gctgttgttt
     9301 actggcaaac tggtttccac ccataacgaa gccagcctct cccgctcgct gaaccgcatt
     9361 atcggtaaaa gcggtgaacc gctgatccgc aaaggtgtgg atatggcgat gcgcctgatg
     9421 ggcgagcagt tcgtcacggg tgaaaccatc gcggaagcgt tagccaatgc ccgcaagctg
     9481 gaagagaaag gtttccgtta ctcttacgat atgctgggcg aagctgcact gactgccgca
     9541 gacgcacagg cgtatatggt ttcctatcag caggcgattc acgccattgg taaagcgtct
     9601 aacggtcgtg gcatctatga aggtccgggc atttcaatca agctgtcggc gctgcatccg
     9661 cgctacagcc gcgcccagta tgaccgggta atggaagagc tttacccgcg tctgaaatca
     9721 ctcaccctgc tggcgcgtca gtatgatatt ggtattaaca tcgatgccga agaggccgat
     9781 cgtctggaga tctccctcga tctgctggaa aatctctgtt tcgagccgga actggcaggc
     9841 tggaacggca tcggttttgt tattcaggct tatcaaaaac gctgcccgtt ggtgatcgat
     9901 tacctggttg atctcgccac ccgcagccgt cgccgtctga tgattcgcct ggtgaaaggc
     9961 gcgtactggg atagtgaaat caagcgtgcg cagatggacg gccttgaagg ctatccggtt
    10021 tatacccgca aggtgtatac cgacgtttct tatctcgcct gtgcgaaaaa gctgctggcg
    10081 gtgccgaatc taatctaccc gcagttcgcg acgcacaacg cccatacgct ggcagcgatt
    10141 tatcaactgg caggtcagaa ctactatccg ggtcagtacg agttccagtg cctgcatggt
    10201 atgggcgagc cactgtacga gcaggtcaca gggaaagttg ccgacggtaa acttaaccga
    10261 ccgtgtcgta tttatgcccc ggttggcaca tatgaaacgc tgctggcgta tctggtgcgt
    10321 cgcctgctgg aaaacggtgc taacacctcg tttgttaacc gtattgccga cacctctttg
    10381 ccactggatg aactggtcgc cgatccggtc actgctgtag aaaaactggc gcaacaggaa
    10441 gggcaaactg gattaccgca tccgaaaatt cccctgccgc gcgatcttta cggtcacggg
    10501 cgcgacaact cggcagggct ggatctcgct aacgaacacc gtctggcctc gctctcctct
    10561 gccctgctca atagtgcact gcaaaaatgg caggccttgc caatgctgga acaaccggta
    10621 gcggcaggtg agatgtcgcc cgttattaac cctgcggaac cgaaagatat tgtgggcttt
    10681 gtgcgtgaag ccacgccgcg tgaagtagaa caggcgctgg aaagtgcggt taataacgcg
    10741 ccaatctggt ttgccacgcc tccggctgaa cgcgcggcga ttttgcatcg cgctgccgtg
    10801 ctaatggaaa gccagatgca gcaactgatt ggtattctgg tgcgtgaagc cggaaaaacc
    10861 ttcagtaacg ctattgccga agtgcgcgaa gcggtcgatt ttctccacta ctacgccgga
    10921 caggtgcggg atgatttcgc taacgaaacc caccgtccat tagggcctgt ggtgtgtatc
    10981 agtccgtgga acttcccgct ggctattttc accgggcaga tcgctgccgc actggcggca
    11041 ggtaacagcg tgctggcaaa accggcagaa caaacgccgc tgattgccgc gcaagggatc
    11101 gccattttgc tggaagcggg tgtaccccct ggcgtggtgc aattgctgcc aggtcagggg
    11161 gaaaccgtgg gcgcgcaact gacgggtgat gatcgcgtgc gcggggtgat gtttaccggt
    11221 tcaaccgaag tcgctacgtt actgcagtgc aatatcgcca gccgcctgga cgctcagggt
    11281 cgccctattc cgctcatcgc tgaaaccggc ggcatgaacg cgatgattgt cgattcttca
    11341 gcactgaccg aacaagtcgt cgtggatgtg ctggcctcgg cgttcgacag tgcgggtcag
    11401 cgttgttcgg cactgcgcgt gctgtgcctg caagatgaga ttgccgacca cacgttgaaa
    11461 atgctgcgcg gtgcaatggc cgaatgccgg atgggtaatc cgggtcgcct gaccaccgat
    11521 atcggtccgg tgattgatag cgaagcgaaa gccaatattg agcgccatat tcagaccatg
    11581 cgtagcaaag gccgtccggt gttccaggcg gtgcgggaaa acagcgaaga tgcccgtgaa
    11641 tggcaaagcg gcacctttgt cgccccgacg ctgatcgaac tggatgactt tgccgaattg
    11701 cacaaagagg tctttggtcc ggtgctgcat gtggtgcgtt acaaccgtaa ccagctacca
    11761 gagctgatcg agcagattaa cgcttccggc tatggtctga cgcttggtgt ccatacgcgt
    11821 attgatgaaa ccatcgccca ggtcactggc tcggcccatg ttggtaacct gtatgttaac
    11881 cgtaatatgg tgggcgcagt ggttggtgtg cagccgttcg gcggcgaagg gttgtccggt
    11941 accgggccga aagcaggcgg tccgctctat ctctaccgtc tgctggcgaa tcgcccggaa
    12001 agtgcgctgg cagtgacgct cgcgcgtcag gatgcagagt atccggtcga tgcacagttg
    12061 aaagccgcat tgactcagcc gctaaatgca ctgcgggaat gggcagcaaa tcgtccagaa
    12121 ttgcaggcgt tatgtacgca atatggcgag ctggcgcagg caggaataca acgattgctg
    12181 ccggggccga cgggtgaacg caacacctgg acgctgctgc cgcgtgagcg cgtgttgtgt
    12241 attgccgatg atgagcagga tgcgctgact cagctcgccg ccgtgctggc ggtgggcagc
    12301 caggtactgt ggccggatga cgcgctgcat cgtcagctag taaaggcatt gccatcggca
    12361 gtcagcgaac gtattcaact ggcgaaagcg gaaaatataa ccgctcaacc ctttgatgcg
    12421 gtgatcttcc acggtgattc ggatcagctt cgcgcattgt gtgaagccgt tgctgcgcgg
    12481 gatggagcaa ttgtttcggt gcagggtttt gcccgtggcg aaagcaatat ccttctggaa
    12541 cggctgtata tcgaacgttc gctgagtgtg aataccgctg ccgctggcgg taacgccagt
    12601 ttaatgacga tcggttaaac aggtagccgg aggatgttac accctcctcc ggcatcttta
    12661 acgtggtcga atcccctcaa taataatccg ctgcacgttt tcaaccgttt gattgaaaaa
    12721 tacctcatcg cgcaacgttg cgcctgtcac cgcctccacc tgaggggcga aatcggcgta
    12781 atgt
//
LOCUS       DAJTOR010000116        12766 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTOR010000116 DAJTOR010000000
VERSION     DAJTOR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.116"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..467
                     /locus_tag="OO249_002887"
     CDS             <1..467
                     /locus_tag="OO249_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCR5637114.1"
                     /translation="ERPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDV
                     PEVVSGEDVTQAEQPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPG
                     ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(476..874)
                     /gene="vapC"
                     /locus_tag="OO249_002888"
     CDS             complement(476..874)
                     /gene="vapC"
                     /locus_tag="OO249_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5637115.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(874..1101)
                     /gene="vapB"
                     /locus_tag="OO249_002889"
     CDS             complement(874..1101)
                     /gene="vapB"
                     /locus_tag="OO249_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5637116.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            1183..1602
                     /locus_tag="OO249_002890"
     CDS             1183..1602
                     /locus_tag="OO249_002890"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5637117.1"
                     /translation="MMSIAQVRSAGSAGNYYTNKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTDDDGRTVRNGADR"
     gene            1589..6460
                     /gene="traI"
                     /locus_tag="OO249_002891"
     CDS             1589..6460
                     /gene="traI"
                     /locus_tag="OO249_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR5637118.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSYAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRYVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAAARMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQA
                     IAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQV
                     PRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYAAIVRDYTGRTP
                     EAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLST
                     WENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTI
                     RVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHID
                     LAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGW
                     TDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDS
                     PARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDA
                     QFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGD
                     IPDNSVQPGAGNGEPVMAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
                     QAVREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
                     VRDLQKEKTPGGD"
     gene            6480..7226
                     /gene="traX"
                     /locus_tag="OO249_002892"
     CDS             6480..7226
                     /gene="traX"
                     /locus_tag="OO249_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5637119.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7281..7841
                     /locus_tag="OO249_002893"
     CDS             7281..7841
                     /locus_tag="OO249_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5637120.1"
                     /translation="MTEQKRPVLTLKRKTEGTATVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7972..8184
                     /locus_tag="OO249_002894"
     CDS             7972..8184
                     /locus_tag="OO249_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5637121.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            8428..8889
                     /locus_tag="OO249_002895"
     CDS             8428..8889
                     /locus_tag="OO249_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5637122.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8935..9144
                     /gene="hha"
                     /locus_tag="OO249_002896"
     CDS             8935..9144
                     /gene="hha"
                     /locus_tag="OO249_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5637123.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            9183..9773
                     /locus_tag="OO249_002897"
     CDS             9183..9773
                     /locus_tag="OO249_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5637124.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQTTLLTEREVSFMKGLFRIVDMTRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            10013..10273
                     /gene="repA"
                     /locus_tag="OO249_002898"
     CDS             10013..10273
                     /gene="repA"
                     /locus_tag="OO249_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5637125.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            10497..10571
                     /gene="tap"
                     /locus_tag="OO249_002899"
     CDS             10497..10571
                     /gene="tap"
                     /locus_tag="OO249_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5637126.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10564..11421
                     /gene="repA"
                     /locus_tag="OO249_002900"
     CDS             10564..11421
                     /gene="repA"
                     /locus_tag="OO249_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5637127.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(12474..>12766)
                     /locus_tag="OO249_002901"
     CDS             complement(12474..>12766)
                     /locus_tag="OO249_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5637128.1"
                     /translation="RLVFQFPIREQAAAFLGVVPVEKYSGTSVRGCPKLSKIGPPEIR
                     AKLYLASLCGLRFNPLMKAMYERLCLRGKAKMCAIGALMRKLVHWCYGVLKT"
BASE COUNT         3100 a         3215 c         3787 g         2664 t
ORIGIN      
        1 aggaacgacc gaaggtcgct ccggagttta ttccgcgtga catcaacccg gaaatggaga
       61 accgtctgag tgccgtactt gccgcaaggg aagcagaagg tcgtcagatg gccagcctct
      121 tcgaaccgga tgtcccggag gttgtttccg gagaagacgt gactcaggct gaacagccgc
      181 aacagccggt gtctcctgtc atcaacgata agaagtcaga ttcaggtgtg aatgttccgg
      241 caggggggat cgagcaggag ctgaaaatga aaccggaaga agagatggaa cagcaactgc
      301 cacccgggat cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc
      361 aggaaaatca tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg
      421 tgcaccggga acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc
      481 tccagtcttc agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa
      541 taatcagccc ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc
      601 gtccctgacg ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt
      661 aatccagaac atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca
      721 tctggctttt ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattc
      781 tcccctggtt caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa
      841 tgcagatgtt ggtatcgagc ataaacttca gcatcagaat gactcccttt cctgcatgcc
      901 gggctgttcc ctgttatcca taaaatcggc gctgacgctg tgtccgtcga accattcgtc
      961 ccatgtctct ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg
     1021 ttttacgttt tccggcaatg caaccgcttt tggcagtctg accgcttggc tgcggttgct
     1081 gagaaatacg gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcca
     1141 ttgtgggatt gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag
     1201 gtcagatcgg ccggaagtgc cggtaattat tataccaaca aggataatta ctatgtgctg
     1261 ggcagcatgg gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt
     1321 gtcgataagg atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta
     1381 agccgcatgc aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct
     1441 cccaaaagtg tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat
     1501 aaccaggccg tggattttgc tgtccgtcat cacccgtgtg gaggcactgg cctccacacg
     1561 gatgatgacg gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt
     1621 taaccacgac accagtcgcg atcaggaacc acagttacac acgcatgcgg tggtgactaa
     1681 tgttacgcag cataacggcg aatggaagac actgagcagt gacaaagtgg ggaaaacggg
     1741 gttcattgag aatgtgtacg ctaatcagat tgcctttggc aggctctacc gggaaaaact
     1801 gaaagagcag gttgaggcgc tgggctatga aactgaagtg gtcggtaagc acggtatgtg
     1861 ggaaatgccg ggtgtaccgg tggaggcctt ttccggacgc tcacagacta tccgggaggc
     1921 cgtcggggaa gacgcctcgc tgaaatcccg ggatgtggcg gcgctggata cgcgtaaatc
     1981 caaacagcac gtcgatcctg aagtcagaat ggccgagtgg atgcagacgc tgaaggaaac
     2041 cgggttcgac atccgggcat atcgtgacgc agcggaacaa cgggcatata cccgcacgca
     2101 gacacccgga cccgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggccat
     2161 tgccggatta agtgaacgca aagtgcagtt tatgtatacg gacctgctgg ccaggacggt
     2221 cggcatactg ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc
     2281 catcagccgt gagcagctta tccccctcga tcgtgagaag gggctgttca cgtccgggat
     2341 tcatatgctc gatgagctgt cagtccgggc actcagtcgt gacatcatga aacagaaccg
     2401 ggtgaccgta catccggaga aaagtgtccc ccggacggcc ggttacagct atgccgtcag
     2461 tgttctggca caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg
     2521 gcagcgtgag cgggtggctg aactggtcat gatggcccgg gagcaggggc gggaggtgca
     2581 gattatcgct gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg
     2641 tgaactgata accggacgtc gtcagttgct ggaaggcatg gccttcacgc cgggcagtac
     2701 cgttatcgtc gaccagggcg aaaaactctc cctgaaagag acgttaaccc tgctggatgg
     2761 tgccgcacgt cataacgtac aggtcctgat aactgacagc gggcagcgaa ccggtacagg
     2821 cagtgcactg atggccatga aggatgccgg ggtgaacaca tatcgctggc aggggggaga
     2881 acagcgaccg gccaccatca tcagtgaacc ggaccgttat gtccgctatg cccggctggc
     2941 cggagatttt gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggagt
     3001 acgggaacag gccatactga cacaggccat tcgcagtgag ctgaaaacac agggcgtgct
     3061 cggacacccg gaggtaacca tgaccgccct gtcaccggtc tggctggaca gccggagccg
     3121 ttatctgcgg gatatgtacc gcccgggaat ggtgatggag cagtggaacc cggagacacg
     3181 cagtcatgac cgctatgtga cagaacgtgt gacggcgcag agtcacagcc tgaccctgcg
     3241 gaatgcgcag ggcgaaacgc aggtggtgcg tatttcttcc ctggacagca gctggtcgct
     3301 gttccggccg gaaaaaatgc cggtggcaga cggtgagcgt ctgagggtga cagggaaaat
     3361 tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc
     3421 gatgacggtt gttgtgccgg gacgggctga accggccacc ctgcctgtga gcgattcacc
     3481 gttcacggca ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagcga
     3541 cagtgcgacg gtttttgcct ccgtcacaca gatggcaatg gacaatgcca ccctgaacgg
     3601 tctggcccgc agtggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc
     3661 ggaaaaactt gcccgccatc cttcctttac ggtggtttct gagcagataa aggcgcgtgc
     3721 cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc
     3781 acagcaggcc attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat
     3841 ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttgctgacct
     3901 gggaggggaa atcaatgcgc agataaaacg gggtgattta ctgtatgtgg atgtggcaaa
     3961 aggctatggc acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct
     4021 tcgccatatt ctcgaaggta aggaggcggt cacgccgctg atggagagag tacctggcga
     4081 actcatggag aaactgacat caggacagcg cgccgccgcc cgtatgatac tggaaacgtc
     4141 cgaccgtttc acggtggtgc agggctatgc cggtgtgggt aagaccacac agttccgggc
     4201 ggtgatgtca gccgtgaaca tgctgccgga gagtgaacgt ccccgtgtcg tggggctggg
     4261 gcccacacac cgtgcggtcg gggagatgcg cagcgccggc gtggatgcac agacactggc
     4321 gtcctttctg catgacacgc agctgcagca gcgcagcgga gaaacgccgg atttcagcaa
     4381 cacgctgttc cttctcgatg agagctctat ggtgggcaat accgacatgg cacgggcata
     4441 cgccctgatt gcggccggtg gcggtcgggc tgtggccagc ggtgacacgg accagctgca
     4501 ggccatcgcg cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggt
     4561 catcatgaag gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat
     4621 taaccgggat gtggaaaggg cgctgtccgg gcttgagcgt gtgaaaccgt ctcaggtgcc
     4681 acgtctggag ggcgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga
     4741 agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt ttccggatgt
     4801 ccccatgaca ctgtatgcag ccattgtccg cgactatacc ggcaggacac cggaagcacg
     4861 ggagcagacg ctgattgtca cgcacctgaa tgaggaccgg cgcgtactga acagcatgat
     4921 tcatgatgca cgggaaaagg ccggagaact ggggcaggtg caggtcatgg tgcctgtcct
     4981 gaacacagcg aatatacgcg acggggagct gcgtcgtctc tccacctggg agaataaccc
     5041 ggatgccctt gccctggtgg ataatgtgta tcaccggatt gccggtatca gcaaggatga
     5101 cgggctgata accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc
     5161 ggtggctgaa ggcgtcacgc tgtacacccc ggacaccatc cgggtgggga ccggtgacag
     5221 gatacgcttc acgaagagtg accgggagcg cggttatgtg gccaacagcg tctggacggt
     5281 gacagcggtt tccggtgaca gtgtcacact ttctgacggt cagcagaccc gtgtgattcg
     5341 ccccggtcag gagcgggcag agcaacatat tgacctggcc tatgccatca ccgcccacgg
     5401 tgcacagggg gcgagtgaaa cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa
     5461 actgatggcc ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca
     5521 ggtgtacacc gataaccgtc agggctggac ggatgccatc aacaatgccg tacagaaagg
     5581 aactgcccac gatgtgtttg agccgaaacc ggaccgggag gtcatgaatg cagagcggct
     5641 gttcagtacg gcgcgggagc tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc
     5701 ggggctggcc gggggagaca gtcctgcacg gtttattgct ccgggacgta aatatccgca
     5761 accgtatgtg gcactgccgg cgtttgaccg taacggcaag tccgccggta tctggctgaa
     5821 cccgctgacc acggatgacg gaaacgggct gcggggattc agcggtgaag gtcgggtaaa
     5881 aggcagcggg gatgcgcagt tcgtggccct gcagggcagc cgtaacggag aaagcctgct
     5941 ggctgataat atgcaggatg gcgtccggat tgcccgtgat aatcctgaca gtggtgtggt
     6001 ggtaagaatc gccggtgaag gccgtccgtg gaatcccggt gccatcaccg gtggccgcgt
     6061 gtggggggat atcccggaca acagcgtcca gccgggagcc ggaaatggcg aaccggtcat
     6121 ggcagaggta ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg
     6181 cgcagatgaa attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac
     6241 agagcaggct gtcagggaaa ttgccgggca ggagcgtgac cgggctgcca taactgaacg
     6301 ggaagccgcg ctgccggaga gtgtactgcg tgaaccacaa cgggtgcggg aagcggtccg
     6361 ggaagttgcc cgggaaaatt tgctgcagga gcgtctgcag cagatggagc gggatatggt
     6421 tcgtgacctg cagaaagaga aaaccccggg cggagactga tacaggaaga caaacactga
     6481 tgacaacgga taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct
     6541 ggttgcagtc atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac
     6601 tggtgctgat ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt
     6661 ttctggccgg gcgaggagcc tttccgcttt ttgccctggt gtgggggctg aatctgtccc
     6721 gtcatgcgca tatccggcaa ccggccatta accggctgtg gggatgggga attattgccc
     6781 agttcgcata ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg
     6841 cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg
     6901 ccatactcct gatggccctg tgggggcctt tgtccggcac cagttacggt attgccgggt
     6961 tgctgatgct ggcagtcagc aaccggctgt accgggcgga agacagagcg gaacgtctgg
     7021 cgctggtcgc ctgcctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
     7081 cggcggtggc cggtcttgtg atgacggtgc tgaccgttgg tctggtgttg tgtgccggaa
     7141 aatcattacc ccgtttctgg cccggagact ttttcccgac gttctatgcc tgtcatctgg
     7201 ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttggccgcaa gaggatgatt
     7261 cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgga
     7321 agacagaggg aacagcgact gtccgcagcc ggaaaaccat catcaatgtc accacgccac
     7381 caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc ggcccgtgaa gcagagctgg
     7441 cggcaaaaaa agcgcaggcc agacaggcgc tgtccattta tctgaacctg cccacgctgg
     7501 atgaggctgt gaataccctg aagccctggt ggccgggatt atttgacggt gacacgcccc
     7561 ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc
     7621 cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc
     7681 tgtgtgccat gaaagccggt gcctgccggt atgacacgga agggtatgtg acggagcata
     7741 tttctcagga ggaggaagcg tatgcgggag cgcgtctggc gaaaattcgt cgccagaacc
     7801 ggataaaggc agaacttcag gccgtgcttg atgagaaata aaaaaagcct cccccagaga
     7861 gaggaggcag ggaaataagg cttaaaagga aataacttcc gcttccgaaa ggaagccagt
     7921 ccactgaatt atttatcaca ataatactga caactaaagg gggacaagaa aatgaatgga
     7981 ttcagaaaca gttcacggaa cggccaggtc tggcgttacc agcgtgccgg cgggcgggcc
     8041 gttattctgg aagtcagtgg acgctggatg gaagcggcag aagcatggcg acgggctgcc
     8101 tgtgttgcgc ctaggacaga ctggcagcag tttgcccgga aaagggctga acactgccac
     8161 cggcgctgcc ggggaagggg gtaacgaaaa aagcagcctt cattactcca ggagtcagaa
     8221 ggctgaaagg agtttatgag atttgcttct taatatccgg tcgggcagta tgaccggaca
     8281 gttctgagta gcacagagtc ttttccgcat taagtcgatt ttttatattt tgtgattaat
     8341 ttcacaaaat aaggtgttat tcaatgtgtg ctgcactatt caggggggct gaaatacagg
     8401 tgccatttat ctgatatgga gaataacatg aggaaatata ttccactggt attatttatc
     8461 ttttcatggc cggtattgtc tgcagatatt catggacggg ttgttcgtgt actggacggt
     8521 gataccatcg aggtcatgga ctcactgaag gcggtgcgga tcaggctggt aaatattgat
     8581 gcaccggaaa agaaacagga ttatggccgg tggtcaacag acatgatgaa atcactggtt
     8641 gcaggaaaaa cggttaccgt cacatatttt cagcgagatc gttatggtcg tattctcggg
     8701 caggtttatg caccagacgg aatgaatatt aatcagttta tggtccgggc cggggctgcc
     8761 tgggtatatg agcagtacaa cacagatccc gtgctacctg tccttcagaa cgaagccaga
     8821 caacaaaaac gtgggctctg gtcagatgct gatccggttc ccccctggat atggaggcac
     8881 cgtaaataaa gcgatggcaa tcctatcatt catctgttct cagcgggaat aaaaatggca
     8941 aaaacaaaac aggaatggct gtatcagtta cggcgatgtt catcagtcaa tacgctggaa
     9001 aaaattattc ataaaaacag agacagttta tcaaacagtg aacgtgaatc atttaattca
     9061 gcagcagacc accggctagc ggaattaatc accggaaagt tatatgacag gataccaaaa
     9121 gagatatgga aatatgtaag gtaataattc agcaataatt aattgccagg agcagataaa
     9181 taatgaagtt gattattttt attctcattg ttctgattat tgcagcgcta cttatccgga
     9241 ttatcctcag aagtgtaaat cagcattctc ctttgctgat gcaattgcat gcggcaggta
     9301 tacggaccgg tgatgcagag cgaatactgt ccagcggtga atactggcaa cgtcagacga
     9361 cgctgctgac agaaagggaa gtcagtttta tgaaaggact gttcagaatt gtggatatga
     9421 cgcggtggta tctgtgtccg caggtacggg tcgcggatat cgtccagctg aacgggaata
     9481 tccggccacg atcacgccag tggtggcagt tattcaggat ggtgtctcag tggcatgttg
     9541 atgtggttat cgttgagctg cgttcgttca gtattgttgc agcagtagag ctggatgatg
     9601 ccagccattt acgaccggaa cgcagacgcc gggatattct tctggaagag gttctgaggc
     9661 aggctggtat tccgttgctc agaagccacg atgccagaaa actgctgcag atgaccggag
     9721 aatggctgaa tacaacaggg gctgatcagc agtcaccgga acatcgtagc tgacgccttc
     9781 gcgttgctca gttgtccaac cccggaaacg ggaaaaagca agttttcctc gctcccggcg
     9841 tttccataac tgaaaaccat actatttcac agtttaaatc acattaaacg acagtaatcc
     9901 ccgttgattt gtgcgccaac acagatcttc gccacaattc tcaagtcgct gatttcaaaa
     9961 aactgtagta tcctctgcga aacgatccct gtttgagtat tgaggaggcg agatgtcgca
    10021 gacagaaaat gcagtgactt cctcattgag tcaaaagcgg tttgtgcgca gaggtaagcc
    10081 tatgactgac tctgagaaac aaatggccgc tgttgcaaga aaacgtctta cacacaaaga
    10141 gataaaagtt tttgtcaaaa atcctctgaa agatctcatg gttgagtact gcgagagaga
    10201 ggggataaca caggctcagt tcgttgagaa aatcatcaaa gatgaactgc agagactgga
    10261 tatactaaag taaagacttt actttgtggc gtagcatgct agattactga tcgtttaagg
    10321 aattttatgg ctggccacgc cataaggtgg cagggaactg gttctgatgt agatgtacag
    10381 gagccagaaa agcaaaaacc ccgataatct tctttaactt tggcgagtca gaaagattac
    10441 cggggcccac ttaaaccgta tagccaacaa tcaagctatg cggggagtat agttatatgc
    10501 ccggaaaagt tcaagacttc tttctgtgct cgctccttct gcgcattgta agtgcaggat
    10561 ggtgtgactg atcttcaaca aacttattac cgccaggtaa agaacccgaa tccggtgttc
    10621 acaccccgtg aaggtgcccg gacgcttccg ttctgcggga aactgatgga aaaggcggtg
    10681 ggcttcacct cccgttttga tttcgccatt catgtggcac atgcccgttc gcttggtctg
    10741 cgtcggcgca tgccaccggt actacgtcgc cgggccattg atgcgctgct gcaggggctg
    10801 tgttttcact atgacccact ggccaaccgc gtccagtgtt ccatcaccac gctggccatt
    10861 gagtgcggac tggcgacgga gtctgctgcc ggaaaactct ccatcacccg ggccacccgg
    10921 gccctgacgt tccttgcaga gctgggactg attacctacc agacggaata tgacccgctt
    10981 atcgggtgct acattccgac cgatatcacg ttcacatctg cactgtttgc tgccctcgat
    11041 gtatcagagg aggcagtggc cgccgcgcgc cgcagtcgtg tggaatggga aaacagacag
    11101 cgcaaaaagc aggggctgga taccctgggt atggatgaac tgatggcgaa agcctggcgt
    11161 tttgttcgtg agcgtttccg cagttaccag acagagctta agtcccgtgg aatgaagcgt
    11221 gcccgtgcgc gtcgtgatgc ggacaggcag cgtcaggata ttgtcaccct ggtgaaacgg
    11281 caactgacgc gtgaaatctc ggaagggcgc ttcactgcca gtcgtgaggc ggtaaaacgt
    11341 gaagtggagc gtcgtgtgaa ggagcgcatg attctgtcac gtaaccgtaa ttacagccgg
    11401 ctggccacag cttccccctg aaagtgacct cctcagaata atccggcacg cgccggaggc
    11461 attcgcacgc ctgaagcccg ccagtgcaca aaaaatcagc gctacataca aaaaacaacc
    11521 tcatcatcca ccttctggtg catccggtcc cccctgtttt tgatacaaaa cacgcctcac
    11581 agacggggaa ttttgcttat ccacattaaa ctgcaaggga cttcaccata aagttacaac
    11641 cgttcacgtc ataaagcgcc agccgtcagc cctacagggt gcaatgtatc ttttaaacac
    11701 ctgtttatat ctcctttaaa ctacttaatt acattcattt aaaaagaaaa cctgttcact
    11761 gcctgtcctg tggacagaca gatatgcacc tcccaccaca agcggcgggc cccgaccgaa
    11821 gccggtttag ttacaacatg caaacataaa cccctaaaaa acccaagtcc gacgcagaac
    11881 tgaaaccaca aagcccctcc ctcataactg aaaagcggcc ccgccccggc ccgaagggcc
    11941 ggaacagagt cgcttttaat tatgaatgtt gtaactatat tatcatcgct gtcagtcctc
    12001 tggctggacg tatcgagtac acgctcgtaa gcggccctca cggccctcac ggcccgctaa
    12061 cgcggagata cgccccgact gcgggtaaac ccttgtcggg ctccgaccgc gcacagaagc
    12121 tctgtcatgg ctgaaaacgg gtatggcttg gcagctacca tgttctgtca agaaaaatcg
    12181 cttgaactcc gacgtacagc cccctaagta ctgccagcaa gctgtcagca ctaagtcccc
    12241 gatacagcgc ggcgtagccg cgctaacggg gttaaaaggg cttacctata cttcatcatc
    12301 gctgaaagcc cgcatggcct cgcagggcag agatgggata ggtgaaacct atcaatacgt
    12361 accggcttac gccgggcttc ggcggtgtta ctcctgtacc atatgtaact acatcatgcc
    12421 gccttacatg cgctacgcgc atatgaagac aaatagccca catcaaacgg tttttaggtc
    12481 ttcagtacgc catagcacca gtgcacgagt tttctcatca gcgcaccaat ggcacacatt
    12541 ttggccttac ctctcaggca taatcgctca tacatcgctt tcatcaatgg gttaaatctc
    12601 agaccacaca acgaagccag atacagtttc gcccggattt ccggcggacc aatttttgac
    12661 agtttcggac accctctgac cgaggtgccg gaatattttt caacgggaac gacacctaaa
    12721 aatgcagccg cctgttcacg tataggaaat tgaaaaacca gacgat
//
LOCUS       DAJTOR010000117        12628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTOR010000117 DAJTOR010000000
VERSION     DAJTOR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.117"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            268..1365
                     /gene="mltA"
                     /locus_tag="OO249_002902"
     CDS             268..1365
                     /gene="mltA"
                     /locus_tag="OO249_002902"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5637129.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1442..2248
                     /gene="tcdA"
                     /locus_tag="OO249_002903"
     CDS             1442..2248
                     /gene="tcdA"
                     /locus_tag="OO249_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5637130.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2299..2742)
                     /gene="csdE"
                     /locus_tag="OO249_002904"
     CDS             complement(2299..2742)
                     /gene="csdE"
                     /locus_tag="OO249_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5637131.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVLGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2742..3947)
                     /gene="csdA"
                     /locus_tag="OO249_002905"
     CDS             complement(2742..3947)
                     /gene="csdA"
                     /locus_tag="OO249_002905"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5637132.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4139..4366
                     /locus_tag="OO249_002906"
     CDS             4139..4366
                     /locus_tag="OO249_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5637133.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4717..5634
                     /gene="gcvA"
                     /locus_tag="OO249_002907"
     CDS             4717..5634
                     /gene="gcvA"
                     /locus_tag="OO249_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5637134.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5653..6048
                     /locus_tag="OO249_002908"
     CDS             5653..6048
                     /locus_tag="OO249_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5637135.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6041..7141
                     /gene="rlmM"
                     /locus_tag="OO249_002909"
     CDS             6041..7141
                     /gene="rlmM"
                     /locus_tag="OO249_002909"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5637136.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENSGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7185..7916)
                     /gene="fucR"
                     /locus_tag="OO249_002910"
     CDS             complement(7185..7916)
                     /gene="fucR"
                     /locus_tag="OO249_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5637137.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICYELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7974..8396)
                     /gene="fucU"
                     /locus_tag="OO249_002911"
     CDS             complement(7974..8396)
                     /gene="fucU"
                     /locus_tag="OO249_002911"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5637138.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8398..9816)
                     /gene="fucK"
                     /locus_tag="OO249_002912"
     CDS             complement(8398..9816)
                     /gene="fucK"
                     /locus_tag="OO249_002912"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR5637139.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERTHAWFFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILIVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     SADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9813..11558)
                     /gene="fucI"
                     /locus_tag="OO249_002913"
     CDS             complement(9813..11558)
                     /gene="fucI"
                     /locus_tag="OO249_002913"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5637140.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEVLQERL"
     gene            complement(11591..>12628)
                     /gene="fucP"
                     /locus_tag="OO249_002914"
     CDS             complement(11591..>12628)
                     /gene="fucP"
                     /locus_tag="OO249_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5637141.1"
                     /translation="IITGLFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETA
                     ANPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPE
                     QLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSR
                     LARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIG
                     RFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIF
                     SLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFI
                     FARFRSQTATN"
BASE COUNT         3023 a         3162 c         3343 g         3100 t
ORIGIN      
        1 ttgaaccgac gaccttcggg ttatgagccc gacgagctac caggctgctc caccccgcgt
       61 ccgtggatgc gcactatact ccgctcgcgt tttcatgcaa cctttttttt accccgatca
      121 cggatttagc atgattttga acaaaaacaa accactaaag cctatttttt gtgcaaaatt
      181 tgcgtcaggg agaggctgct catttcatta aacgcggcgg tttgttatct tcgttgcgcc
      241 ttatttttta acctgaagaa gagaacaatg aaaggacgtt gggtaaagta ccttcttatg
      301 ggcacggttg tggcaatgct tgccgcctgc tcttccaaat caaccgatcg cggacagcaa
      361 tataaagacg ggaaatttac ccagcctttc tctctggtga accagccaga tgccgttggc
      421 gcgccgatta acgccggtga ttttgccgag caaattaacc atatccgtaa ttcgtcaccg
      481 cgtctgtatg gcaaccagag taatgtttat aacgcggtgc aagagtggct gcgcgcaggc
      541 ggtgataccc gcaatatgcg ccagttcggc attgatgcct ggcagatgga aggtgccgac
      601 aactatggta acgtgcagtt tacaggctat tacacgccgg taattcaggc gcgccatacc
      661 cgccagggcg agttccagta tcctatttac cgtatgccgc caaaacgtgg tcgtctgccg
      721 tctcgtgcgg agatctacgc gggggcattg ggcgataaat acatcctcgc ttacagcaac
      781 tctctgatgg ataacttcat tatggatgtg cagggtagtg ggtatatcga ctttggtgat
      841 ggcagtccgc ttaacttttt cagctatgca gggaaaaacg gtcatgccta tcgcagcatt
      901 ggtaaggtgc tgatcgaccg tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt
      961 cgtcattggg gcgaaacaca cagtgaagcc gaggttcgcg agctgctgga acagaacccg
     1021 tctttcgtct tctttaaacc gcaatctttt gcaccggtga aaggggcaag tgcggtgccg
     1081 ctggttggcc gtgcttctgt tgcctccgat cgttcaatta ttccgccagg tactaccttg
     1141 ctggcagaag tgccgctgct ggataataac ggcaaattta acggtcagta cgaattgcgt
     1201 ctgatggtgg cgctggatgt cggtggcgca atcaaaggcc aacacttcga tatctatcaa
     1261 gggatcgggc cggaagccgg acatcgcgca ggttggtaca accactatgg acgtgtctgg
     1321 gtgctgaaaa ccgccccggg cgcaggtaac gtctttagcg gctgatgtgt tattctgtat
     1381 accgttttta cggatacgac agggtgaggg ataacctcac cctttttaat tctgaggtgc
     1441 tatgtctgtg gtaattagtg atgcatggcg tcagcgtttt ggtggcacag cgcgtctgta
     1501 tggtgaaaaa gcgttgcaac tgtttgctga cgcgcatatt tgtgtggttg gtatcggtgg
     1561 tgtcggttcc tgggcggcgg aagcgctggc gcgcacgggg attggcgcaa tcacgcttat
     1621 cgatatggat gatgtgtgcg tcaccaatac caatcggcaa attcacgctc tgcgcgataa
     1681 cgtcgggctg gcaaaagcgg aagtcatggc ggagcgcatt cgccagatta acccggagtg
     1741 ccgtgtaacg gtggtggatg atttcgtgac gccggataac gtagcgcaat atatgagtgc
     1801 cggttattcg tacgtgattg atgccattga tagtgtacga cccaaagcgg cgctgattgc
     1861 ttattgtcgg cgcaataaaa tcccgctggt cacgaccggt ggcgcgggtg ggcagattga
     1921 cccgacgcag attcaggtta ccgatctggc gaaaacgatt caggacccgc tggcggcgaa
     1981 gttgcgcgag cgcctgaaaa gcgattttgg cgtagtgaaa aacagtatag gtaagctcgg
     2041 cgtggattgc gtgttttcta ctgaagcgct ggtgtacccg cagtcagacg gtacggtgtg
     2101 tgcgatgaaa gccacggcag aagggccgaa gcggatggat tgtgcatctg gatttggcgc
     2161 ggcaacgatg gtgaccgcca cctttgggtt tgttgcggtt tctcatgcgc tgaagaagat
     2221 gatggcgaaa gcagcgcgtc agggttaaac aatcaggtcg gatgcgacgc gaacgtctta
     2281 tccgacctgg ttctgtgctt aaacctgctt cgccgcagcg ataattgcct cgcttaacgc
     2341 attcaacccc tggctgcgtg aggcgctaag ctgcccacgc aatcccagct catcaaacaa
     2401 tgccagcggc gactgtgcct gtaattcggc ggcggttttc ccctcaacgg cagtcaacaa
     2461 caccgccaac aggccgagca caatgcgccc ttcgctgtcg ccaaagaagt gcattttacc
     2521 gttttcagcc acggtatatc ccagccagac gcggttttcg catccggcaa tctctttagc
     2581 ctgcgctttt aactcgtctg gcaaagccgg aagctgtttc cccagcatga tcaactggcg
     2641 atatttatct tcccattgcg tcaacggtgc gaaggtattg cgtaacgttt ctgcggttac
     2701 ggttgtgccg aacggatgtc cggcgaattg cgggtttgtc attaatccac caataattcc
     2761 agcgcgcggt caacggcatt caccagcgca tccacatcac tctttgtatt atatggcgca
     2821 aaagaggcgc gcagtgtgcc ggttacgcct aattctgcca gtagcggctg agcacaatgt
     2881 tgcccggccc gcaaggcaat accgtactcc gccagtagcg tcaccatatc gctgtgatga
     2941 acgccagcaa aatcaaaggc cagcaggctg gaatcctggc agcggaatga acgaaagcct
     3001 ggacgtttcg ccagcgcatc ttccgccagc gttgctaagc tatggctcca gttttcggcc
     3061 tggttgatat cgtaatctgc cagccattcc agcgccgcgc ttaatcctat gacaccagcc
     3121 acatttggcg ttcctgcttc cagtttccac ggcgcagatt gagtcgtgaa gccgtcaaaa
     3181 ctcacttcgt gaatcatttt gccgccaccc agccagggcg acatcgcttc cagcagttct
     3241 gatttgccat acagcacgcc gatacccgtc gggccataca gtttgtgacc agagaaagca
     3301 tagaaatcga tatccagttg ctgaacatcc gcggggaaat gcaccgcccc ctgagcacca
     3361 tcaaccatca ccaccatccc tgctgaatga gcaaaggtaa tcgctcgcgc cagatccggg
     3421 caaccgccag taacgttcga catctgactc aacgccagaa tccgactacg gggagtaatc
     3481 agttctggca acaaatcgac atccggcagt cgctgcgcat taagcggcaa tttcaccact
     3541 ttggctccag tttgttgggc gaccatcagc caggggacga ggttggcgtg gtgttctgcc
     3601 acgctgacaa taatctcatc gcccggttgc agacgcggac gcgcatagca ttgtgccacc
     3661 atgttgatgg attcagtggt gccgcgcgtc cagacgatag ttttatcatc cggtgcattc
     3721 agtaattgcg ccactttctc tcgtgcagct tcataacgcg cggtcaggcg ttgggcttcg
     3781 gcaaactggc tgcgatggac gtttccggcg ctcaggctgt aaaactgtcg agtggcttca
     3841 accacggctt caggtttaag cgcggtcgcg gcgctgtcga gatagacgcc cgcatcctgt
     3901 agtgcgggaa actgggcgcg aaactgcgcg ggattaaaaa cgttcatggt actcctcggc
     3961 ttgatgcacc gatcgtgacg caaaattcac tttgatacaa ggagagtgct aaccatcaga
     4021 attatctgga tatcctggcg aagccgcgta agtaacatat gctgaatagt gttaggcgtt
     4081 aattttagcc tgttaatcaa tactattaag atttattaat cgctttaagg agaagatgat
     4141 gaaaaagact gccgcaatta tttctgcctg tatgctgact tttgccctga gcgcctgttc
     4201 cggttcgaac tatgtgatgc acaccaatga cggacgtacc atcgtctctg acggcaaacc
     4261 acagactgat aacgataccg gtatgatttc gtataaagac gctaatggca acaaacagca
     4321 gatcaaccgt actgacgtga aagagatggt cgaactggat cagtaattcg cgatcgcaag
     4381 gtaaaaaaaa gcaccgcaat taggcggtgc tacattaatc actatggaca gacagggtaa
     4441 atgtacagga agtgaaaaaa ggtagctttg ctaccatggt ctgaatcgca gaccaattgc
     4501 aaacacaaca acacaacatc acaaccgtaa gccaaaagtt caccagaaca cgcattccga
     4561 taaaactttt cgttccggct caggaagtgc cgccactata ggtatttgct ggtagaagct
     4621 caacggacaa tttataatgg ctcagattaa aaaaactaat aggttacata gtgtgatcta
     4681 attgttaaat tcatttaaca tcaaagttta atagccatgt ctaaacgatt accaccgcta
     4741 aatgccttac gagtttttga tgccgcagca cgccatttaa gtttcactcg cgcagcagaa
     4801 gagctttttg tgacccaggc cgcagtaagt catcaaatca agtctcttga ggattttttg
     4861 gggctaaaac tgttccgccg ccgtaatcgt tcactcctgc tgaccgagga agggcaaagc
     4921 tatttcctcg atatcaaaga gatattttcg caattaaccg aagcgacgcg taaactccag
     4981 gcccgtagcg ccaagggggc gttgacggtc agtttactcc ccagtttcgc cattcattgg
     5041 ttggttccgc gactttccag ctttaattca gcttatccgg gaattgacgt tcgaatccag
     5101 gcggttgatc gtcaggaaga taagctggcg gatgatgttg atgtggcgat attttatggt
     5161 cggggcaact ggccggggct acgggtggaa aaactgtacg ccgaatattt attgccggtg
     5221 tgttcgccgc tactgctgac tggcgaaaaa cccttgaaga caccggaaga tctggctaaa
     5281 catacgttat tacatgatgc ttcgcgccgt gactggcaga catatacccg acagttgggg
     5341 ttaaatcata tcaacgttca gcaagggcca atttttagcc atagcgccat ggtgctgcaa
     5401 gcggctatcc acgggcaggg agtggcgctg gcaaataacg tgatggcgca atctgaaatc
     5461 gaggccggac gtcttgtttg cccgtttaat gatgttctgg tcagtaaaaa cgctttttat
     5521 ctggtttgtc atgacagcca ggcagaactg ggtaaaatag ccgcctttcg ccaatggatc
     5581 ctggcgaaag ccgctgctga acaagaaaaa ttccgctttc gttatgaaca ataatttacg
     5641 tagggtacga ccatgaccag ccgttttatg ctgattttcg ccgccattag cggcttcatt
     5701 tttgtggctc tgggcgcttt tggcgcgcat gtgttaagta aaaccatggg agccgttgag
     5761 atgggctgga tccagaccgg cctcgaatac caggcgtttc atacgctggc gatcttaggt
     5821 ctggcggtgg caatgcagcg tcgcatcagt atctggtttt actggagtag cgttttcctc
     5881 gcgttaggca cggtgttgtt cagcggcagc ctttattgcc tggcgctgtc ccatctgcgt
     5941 ttgtgggcgt ttgtcactcc ggttggcggc gtgagcttcc tcgcgggctg ggcgttaatg
     6001 ttagttggtg ctatccgttt aaagcgcaag ggcgtaagtc atgaataagg ttgtattgct
     6061 gtgccgtccg ggctttgaaa aagagtgcgc cgcagaaatt accgataaag ccggccagcg
     6121 ggaaattttc ggttttgccc gcgtgaaaga gaattcgggt tatgtcattt atgaatgtta
     6181 tcaacctgat gatggcgata agttaatccg tgagctgccg ttcagttcat taatttttgc
     6241 ccgccagtgg tttgtggtgg gggaactcct gcagcatttg ccgccagaag atcgtattac
     6301 ccccattgtc ggcatgttac agggcgtagt agagaagggc ggtgaactgc gtgttgaagt
     6361 tgccgatacc aacgaaagca aagagttgct gaaattctgc cgtaaattta ctgttccgct
     6421 acgcgctgcc ttgcgcgatg ccggggtgct ggcgaactat gaaacgccga agcgtccggt
     6481 tgtgcatgta ttctttattg caccaggctg ctgctatacc ggttactcat acagcaacaa
     6541 taattcgccg ttctatatgg gcattccgcg cctgaaattt ccggcagagg cgccgagtcg
     6601 ttccacgctc aaactggaag aggcatttca tgtgtttatt cctgcggatg agtgggatga
     6661 acgcctggcg aacgggatgt gggcggtgga tttaggcgct tgccctggcg gctggaccta
     6721 ccaactggtg aagcgcaaca tgtgggttta ttccgtcgac aacggcccga tggcgcaaag
     6781 tctgatggat accgggcagg tgacgtggct gcgggaagac ggtttcaaat tccgtccgac
     6841 acgcagcaat atctcctgga tggtatgcga tatggttgaa aaaccggcta aagttgcggc
     6901 attgatggcg cagtggctgg ttaatggctg gtgccgtgaa accattttca atctcaaact
     6961 gccgatgaaa aaacgctacg aagaagtatc acacaatctg gcgtatattc aggcacagct
     7021 tgatgaacat ggcataaatg ctcagattca ggcgcggcag ttgtatcacg atcgcgaaga
     7081 agtgacggtg cacgtccgcc gcatctgggc tgcggtgggt ggtcgtcgcg acgagcgata
     7141 acaggcaagg ccggggcgtc gtcttcggcc tgctaccctt tccgtcaggc tgttaccaaa
     7201 gaagttgcaa cctggcgctc atcagaaata atgtgcgtta cctcatccag atgcccgatg
     7261 cgggcttccc ctgaacgatt aaatttactt ttatcaatca ataacaacga ttgcgcggca
     7321 cgttttaata gcatcgattt gtaatcagcg ttgatcgcat tggagtccca cagtgcgccg
     7381 ctgctatcga tcccttcaca agaaaaaata aacagatcga tttccagcga tttaagttgg
     7441 gaaatcagcg agggattgac gtaacagcca tatttgcgct caagtgtgcc gccggaactg
     7501 atcagttgaa tgcgttcgcg tttaccgagt tcatagcaaa tcggatggct attggtgaag
     7561 acctgaatgt tgatgtcagg caactggcgt gccagatacc agcaagttga actggcgtct
     7621 aaggctatca ccatcccttc ttcaatccac gcgagcgcct cgcgcgcgat atctgcttta
     7681 tgcgcataat ggcttttcag cctgatgtga aagggatcgc cactgtcttg attttgacgg
     7741 tggatatatt tagcgcgtcc atgattgcgc agaattttac cctgcgtctg taattcattg
     7801 agatcgcgac gaatggtttc tttactgacc tttagctgtt cagagagagc ttccgtggtc
     7861 aggctggtat ggttcagcag caggtcgact atcgcttgct ggcgtgccgc tttcatattt
     7921 ttcaccccat cattcgataa ccgcccgcgt catgcgggcg taccggtatg agattacggt
     7981 gttacccctt tttttaaaag aatattcccg tacttcgctc gttcgcctgt gataacgatc
     8041 gcaaaggctt tttgcgcccg ttcataaaac gcaaaacgat tgatgcggat gatgtcagga
     8101 cacggggctt gtagtgaaag cgcattacgg taacgtcgtt ctacttcagg atcgagagtg
     8161 tcaccttcta ccgccgccat catcaccagc ggcggtgcat aactgtccag ttcaaataac
     8221 gggataatcg cctggagcaa gtcgctcacc aacaggccat cagcgcggat cacctgcggt
     8281 cccatcgaat gggcgggaaa gtgagcatcg gaaaaaataa tttcatctcc atgtcccatc
     8341 tctgccagca ctttcaatag ttcgggagaa attaacggcg aaattgtttt cagcatttca
     8401 cacttcctct ataaattcag gttcagtttg cgggtagaaa taacggtact gataatgaat
     8461 ctgtgcgcgg gcttcttcag ggctgttaaa ttcccctacg ccataccaac cgaacagcgc
     8521 agctcctgcg acggtcgttt cggcgtcgtc gagaactttt accggaatat caagcatatt
     8581 ggctttaatc tgattccaca atgtgttgcg acttcctcca ccgactaaca ataattcaga
     8641 ggccttaaag tgcccgattt tttccagcat ctgtagattg cgctgtaatt gcgcagttaa
     8701 cccttccagc gccgcgcgat agaaatgccc ccgcgtggta ttaagcgtca ctccttgcca
     8761 gccagcgttc tgacacgaca ataaatcaca ctgcattttt acgccatccg cgctaggcgc
     8821 gataagacga gcttcttcaa tcaacatttg ccagggtgtt tcagccgtcc agaacagttt
     8881 tctcacccat tccagcacgc cggatgccag ccattgcata cctgggttat acaaccctgc
     8941 ctggctatcc agttcgcagg tggaaccggc gtactgactt aacagcgaag tatcaacctg
     9001 ggcgctacgg actattaaaa tttcccatgt accggaagag agcacgggtt cattttggtc
     9061 ggcaccagcg ccaaaaaggg cgaactgggt atcgtgacct gcggaaatca ccggtatgcc
     9121 aacgggtaag ccgagcattg ctgcggcgct gttctgtagc gtaccaatct gttcacccgc
     9181 ttccaccaga cgagggaaga gtcggcgtgg aataccagtg gcttgtaaaa tttgcggact
     9241 gaaatcgcgt tgctggatat ccagcatctg gctggttccg gccatcgtga tatcagtagt
     9301 gaattcgccg gttaaacggt ggttaatcag cgacgaaata aagaaccagg cgtgcgtgcg
     9361 ttccagcagt tgtggatgat tttctttcaa ccacaccaac ttatataacg tattgaaact
     9421 aaaggctccg acgccagaaa tagcctgcaa ccgctgtgcg gagatcaact gttcaatatt
     9481 gtccataacc gctgctgttc gcggacattt ccagctaatg atcggataga gcagattgcc
     9541 ttgcttatct accagagcgc catccacacc aaaggtggtg acggcgatac cgcggatgtg
     9601 gcattcagtc agttcactat tgatttgccg acagcaatca gcaaagcgtt gcaaaatggc
     9661 gtctaaagac cactggtgcc aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt
     9721 tgaggcgcgg gcaacaattt ttccctgccg attaaccgcg atggccctga cattggtcgc
     9781 gccacagtcg agtaccagga taacttcttg tttcataatc gctcctgcaa tacttcaata
     9841 tccatgccgt gcgcagccca ggcagaagga cgatacactt tggtctcttc aacgttgtgc
     9901 atacataccg ggatacgcag catggaggcg agagtgataa agtcagcgcc aacgtggccg
     9961 atggtcagaa ccccatggtt agcgccccag ttcgccatta ccgagtacac atccgtaaac
    10021 ggccctttac cggtgaggcg cggtgcaaac caggtggttg gccaggttga gttggtgcgt
    10081 ttgttgagga tgtcatgcac atccttcggc aattccacgc tccagccttc cgcgatttgc
    10141 agtaccggtc ccaggccttt gatgatgttg acacgagtca tggtgaacgg gacgccgcct
    10201 tcggtaagga agcgggaaga gtaaccgccg ccacggaagt attcgtggat cgccgggcac
    10261 cattcggtag cggcgaggca agcgtcagcc tcttgctgag agatttccca gtgcggcttc
    10321 atcgtcgggt taccttcgct gtcgcgttgt ttacaggaac cgtccagcgc agcagaacct
    10381 gagttgatca aatggatgat gccgtgttct gccagtccat cgagtttatg ccccgttaca
    10441 cgctcaattg cttctggtga ccagtaggta cgcacatcgg caaatacctg agcggtgccg
    10501 gtgagctggt gacccattag cattgccacg ccgttaagac tgtcgttttc ggtcgccacg
    10561 acaaagggtt cgcgcacgcc attccagtca aatgaactgt tgaggatcgc ttcggcggtg
    10621 tcaccattgg gatattgatc ggtccagtga cgttgcccct ggaagcccgc agcgatggcg
    10681 ttgtagccaa gtgattcttc cacgcgacca atatcggcca gtttgctgtt gccttgcatc
    10741 atgtcgcgga tacacatcgc catcagtaaa ctttcgcgca gaactgcgcg gctttgctcg
    10801 gcattgcgtt gatactgttt gttattttca tcttcgccat agcggaagtt tttatcagcc
    10861 caggccagtg ccatttccag ttcggcttcg tcgtaaatct tctgatcgat acggcgacgc
    10921 agttcggtca tatccaccgc ctggactttc attcccagcc aggattcaaa gaagttgtga
    10981 tcaacgatgg aaccggcgat acccatcgaa acgccgccca gcgacagata gcttttacct
    11041 ttcatgctgg cgacggccaa accggcgcgg gcaaagcgca gcagtttttc ttcaacatcg
    11101 gcaggaatcg atgtgtcatc ggcatcctga acgtcatgac cgtaaatgga gaatgccggg
    11161 atgcctttct ggctgtgagc tgccagagcc gctgccaggt aaacagcgcc gggacgttca
    11221 gtgccgttaa agccccaaat ggctttcggg cgggttggat ccatgtcgat ggtttcactg
    11281 ccatagcacc agcaaggcgt taccgtaatg gtgaggccta cattctggct gctgaatttt
    11341 tcttcgcaag cagcggcttc agccataccc gcgatacagg tatcggaaat gacacactcg
    11401 acggcagctc cgcaggcatg gcgcagtttc tcggtcagca gtgcggccgt agctttcgcc
    11461 atattcattg tttgttcttc aagcgactca cgaacaccca tgcgacgacc gtcaataacc
    11521 gggcggatac caattttcgg taagctgatt tttttcatta cagattcctc actttattcg
    11581 gaaaatatgt tcagttagtt gccgtttgag agcggaaacg ggcaaagata aagatgaccg
    11641 cgaagcagag tgcggggatc agttcagcag tggggatgtt gcccgccgcg tcactgacaa
    11701 aacccatgac cggagtgaca ataccgccgc caataatggt cataacgatg aaggacgaac
    11761 cgtatttggt gtcctggccg agattcttaa tgcccagcga gaagattgtt gggtactgaa
    11821 tcgacataaa ggcgctgcat aaagtcaggg ctattaagcc cacatgaccg ccagcgaagg
    11881 ctgagatcag gcacagtgcc atagcgatta atgcgtaggc ggccaggact ttgtgtggtg
    11941 cgaagcgact gatgagccag gtaccggtga aacgaccaat aaagaagcac accatggttc
    12001 cggttaaata gttagcggca aagcctgcag tcatacctgg aatttcttct acagcgtagc
    12061 gaatcaaata gctccagcag gccgtttgtg cgccaacgta gcagaattgc gctaatactg
    12121 cccagcgcca gtggcgaata cgcgccaggc gagaaagcga tgcggagaac gatccttgtt
    12181 tggcgtcact gtgattatca ctctgcaatg ccgggaattt cgtcagcatg atcagcaggg
    12241 cgaccagtaa cacgatagcc acgatgatca tataaggtgt ctgtaccgat aataccaggc
    12301 tgtgtttata cgcactcaat tgctctggag acattttatc gagaacgtct tgcgattgat
    12361 gtggcacgtt agacaaaata agactttgcc caaagacaac cgcgataatt gcgccaaacg
    12421 agttaaatgt ttgcgcaaga tttaagcgga agtgaccact gctttccggc cctaataccg
    12481 taacaaaagg gtttgcggca gtttccagac aacctaatcc ggctgcaata ataaataggc
    12541 caactaaaaa taaggtgtag ttcattattt ctgcagcggg ccagaataat gcagcgccca
    12601 gggcatataa aaataaccca gtaataat
//
LOCUS       DAJTOR010000118        12561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTOR010000118 DAJTOR010000000
VERSION     DAJTOR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.118"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..456)
                     /locus_tag="OO249_002915"
     CDS             complement(<1..456)
                     /locus_tag="OO249_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5637142.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETG"
     gene            complement(486..1202)
                     /locus_tag="OO249_002916"
                     /pseudo
     CDS             complement(486..1202)
                     /locus_tag="OO249_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            1498..2274
                     /gene="exuR"
                     /locus_tag="OO249_002917"
     CDS             1498..2274
                     /gene="exuR"
                     /locus_tag="OO249_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5637143.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            2619..3281
                     /locus_tag="OO249_002918"
     CDS             2619..3281
                     /locus_tag="OO249_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5637144.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            3285..3668
                     /gene="mzrA"
                     /locus_tag="OO249_002919"
     CDS             3285..3668
                     /gene="mzrA"
                     /locus_tag="OO249_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5637145.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3815..4183
                     /locus_tag="OO249_002920"
     CDS             3815..4183
                     /locus_tag="OO249_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR5637146.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            4221..4526
                     /locus_tag="OO249_002921"
     CDS             4221..4526
                     /locus_tag="OO249_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5637147.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            4529..4933
                     /locus_tag="OO249_002922"
     CDS             4529..4933
                     /locus_tag="OO249_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5637148.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4923..5222
                     /locus_tag="OO249_002923"
     CDS             4923..5222
                     /locus_tag="OO249_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5637149.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            5408..5800
                     /locus_tag="OO249_002924"
     CDS             5408..5800
                     /locus_tag="OO249_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5637150.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSKFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5870..6773
                     /locus_tag="OO249_002925"
                     /pseudo
     CDS             5870..6773
                     /locus_tag="OO249_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            7066..7431
                     /locus_tag="OO249_002926"
     CDS             7066..7431
                     /locus_tag="OO249_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5637151.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7673..8029
                     /locus_tag="OO249_002927"
     CDS             7673..8029
                     /locus_tag="OO249_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5637152.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGVWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(8080..8976)
                     /gene="yhaJ"
                     /locus_tag="OO249_002928"
     CDS             complement(8080..8976)
                     /gene="yhaJ"
                     /locus_tag="OO249_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5637153.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            9081..9782
                     /locus_tag="OO249_002929"
     CDS             9081..9782
                     /locus_tag="OO249_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5637154.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9805..9969
                     /locus_tag="OO249_002930"
     CDS             9805..9969
                     /locus_tag="OO249_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637155.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(10103..11413)
                     /locus_tag="OO249_002931"
     CDS             complement(10103..11413)
                     /locus_tag="OO249_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5637156.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLEDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11441..12561)
                     /locus_tag="OO249_002932"
                     /pseudo
     CDS             complement(11441..12561)
                     /locus_tag="OO249_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3219 a         3171 c         2997 g         3174 t
ORIGIN      
        1 gcctgtctcc agaacacctt tggtggtttg agcaaacatc aggtttttag ccagagaacc
       61 gttagttaac gcatcggaag caaagatttt ggttgcggta aaagtggttg ccgcatcagc
      121 tttaatttct tcaccgcccc aggttactgt cagcggaaca atcttgctag catccaggct
      181 ttgaaccagt tgcgccgggg aaactaactg catttttaca tctttggtaa cgtcgttaga
      241 ccagattttg gtcatcaact ggtaagattg cagaccctgc cccggcagat attgcatttc
      301 tactgctttc ggcaacgcgg tgttatcggt ctgtgtcata tccagagcgg catcaacgtt
      361 ggccgttaca gtaatatcct tttgtactgc caatgcggag cctgcaacag aaaacatcgc
      421 tgcgaccaga agagattttg caaatacctt tttcattact attccttcgt ttattacaat
      481 gataattaat ggaccgccag ttcaaactcg ccattatctt tggtgatcca gttattatat
      541 ttgacaacga ttttgtcgta gccagatatg ctttcaagat ttctttcctg accagggaag
      601 atgtttttat ttacggtctg tatcttacaa ttttctttgc tattattttt acaataagcg
      661 atcgttaaaa tctttaagcg ttgattccct gcattaacta atttttgggc agcatttact
      721 tctaacttag caataggttg ctgaggtgga acactaacaa caatccccca aattaagcta
      781 acagttaact gcatagagaa atctttttta tctgtggcaa cgtcatcaat actgggaacc
      841 gcttcaaatt taacgcgtta atttttttct ttttgctctg gttccattgc aacaaagcgc
      901 acagttttgc tactaccggc tggtaaagcg aatttaggtg gcataacaac cagatgattt
      961 gcatcaccgg atttaatctc aacttcattt tcctgaggcg tcgatggatt atcgatacgg
     1021 aatactgtcg ccttgatgta ctgaacttca ttacttttag atattacgcg aacgttgcct
     1081 tcatcctgac tattaatgga aacagccata ggataaacag tcatatttga ctgtgccaaa
     1141 aaaggagcga acacgaataa aagcggcgca agccgtgata agagattaat tttcgccacc
     1201 atataaattt gatcttacaa tattgtttcc aaaaagttgg tgtattagag atgtagattt
     1261 attgtaagtc aatataaaaa agcattttta gttaatatta aatttaaagg ctgggaatta
     1321 acttgctgaa atttagtagc tttcacaatg acgatactta ttttttagga atttataaat
     1381 attcacaata ttaattcaat acattaattc aatcaccaaa tttatatatg accaatggca
     1441 tacccatgaa taaagtggta tgataacttc gtccgcattc cactccggga gacgcatatg
     1501 gaaatcactg aaccacgccg tttgtatcaa caacttgccg ctgacctgaa agagcgcatt
     1561 gaacagggcg tctatctggt gggtgataaa ctgcctgccg aacgctttat tgccgatgaa
     1621 aagaacgtca gccgaacggt agttcgtgaa gccatcatca tgctggaagt tgaaggctat
     1681 gtggaggtgc gtaaaggttc aggtattcat gtggtttcca accagccgcg ccatcagcag
     1741 gcggctgaca ataatatgga attcgccaat tacggtccgt ttgagttgct tcaggctcgc
     1801 cagctcatcg aaagtaatat tgccgagttc gcggcaactc aggtaacgaa acaagacatc
     1861 atgaaactga tggccattca ggaacaggcg cgcggcgaac aatgcttccg tgattccgag
     1921 tgggatttac agttccacat tcaggtagcc ctggcgacgc agaactccgc cctggcggcc
     1981 atcgttgaaa aaatgtggac ccagcgtagc cataacccgt actggaaaaa actacacgaa
     2041 cacattgatt cccgtaccgt tgataactgg tgtgatgacc acgatcaaat tctcaaggcg
     2101 ttaattcgca aagatcccca tgctgctaag ctggcaatgt ggcagcatct ggaaaacacc
     2161 aaaatcatgt tatttaacga aaccagcgac gatttcgagt tcaatgccga ccgctatcta
     2221 ttcgccgaaa acccggtagt gcatctcgat acggccacca gtggcagtaa atgaaactcc
     2281 tgtccgacag gcgctgcgac gcgcctgttc gcttcgtcgg taagcgaaaa gtataaagtg
     2341 tcagcctgtg taaatcctct cgccaccctc ccctgcattc agcaaaatca gactccacgg
     2401 acatgtaatt ttgataacga acaacgttga cctttgttac aattagattc aatttgaatt
     2461 tatgtttttg aatgctttct tatctcacga tttaacaggg aatagttcag gctgtgttga
     2521 tgtatcaaac ccgcagaaca taccaaaaca gcaataacat tgcggtagtg catcttttaa
     2581 aaccagcgtg gcgttaaccg attcaccagg aataatgaat ggaacttttg acccaattgc
     2641 tgcaagccct gtgggcgcag gattttgaaa ccctggccaa tccatcgatg attggcatgt
     2701 tgtattttgt cttgtttgta attttgttcc ttgaaaacgg cttgcttcca gcggcctttt
     2761 taccgggcga cagtttactg gtattggtcg gcgtgttgat tgcgaaaggc gcgatgggtt
     2821 acccgcaaac gattctgctg ctgaccgttg ccgccagcct cggctgctgg gtcagctata
     2881 ttcaggggcg atggctgggc aatacccgca ccgtacaaaa ctggctatct catttacccg
     2941 cgcattatca tcaacgcgca caccatcttt ttcataaaca cggtttatcg gcgctgttaa
     3001 ttggtcgctt tattgcgttt gtcagaacac tgctgccgac gattgccggg ttatcagggc
     3061 tgaataacgc gcgctttcag tttttcaact ggatgagcgg tctgctgtgg gtattgatcc
     3121 tgacaactct gggttacatg ctcggcaaaa cgccggtatt tttaaagtac gaggaccagc
     3181 tgatgtcatg cctgatgctg ctcccggtgg tgctgctggt gtttggcctg gcaggttctc
     3241 tggtcgtgtt atggaaaaag aaatatggaa atcgggggta agggatgcaa atacctcgca
     3301 tgtcgcttcg ccagctagcc tggtccggca ctgttttact tctggtcggc acgctgttgc
     3361 tggcctggtc cgcggttcgc cagcaagagt ctacgctggc gattcgtgcc gttcatcaag
     3421 gcacaacgat gccagacggt ttttcgatct ggcatcacct tgacgctcat ggcattcctt
     3481 tcaaaagtat cacccccaaa aacgacactc tgttaattac atttgactcc agcgaccaga
     3541 gcgccgccgc aaaagcggtc ctcgacagaa cattgcccca tggctacatc attgcgcagc
     3601 aggacaataa cagtcaggct atgcagtggc tgacccggtt acgggataac tctcatcgct
     3661 tcggataact tcctgttatc cgaaacatat cactcacttt ggtgatttca ccgtaactgt
     3721 ctatgattaa tgaggcggtg gttaacacta ccgcttcgtt gtctggacaa tcgttccttt
     3781 gtaacaggtc cgaatcacaa tggaaggttc aagaatgaaa taccgcatcg ctttagctgt
     3841 ttctctcttt gctcttagtg ccggtagtta tgccactacc ctgtgtcagg aaaaggagca
     3901 aaatatcctt aaggagatca gctatgccga aaaacaccaa aaccagaatc gtattgacgg
     3961 tctgaataaa gccctgagtg aagtccgggc caactgttca gatagccagc tgcgtgccga
     4021 tcatcacaag aaaatcgcaa agcagaaaga tgaggtggcg gaacgccagc aagatttagc
     4081 cgaggcgaag caaaaaggcg atgccgataa gattgccaaa cgcgaacgga aactggcaga
     4141 agcgcaggaa gagctgaaaa agctggaagc gcgcgactac taactcacaa tagtcactac
     4201 ttactcacct ggagaaaact atgtcgaaag aacacactac ggaacatctg cgtgctgagt
     4261 tgaaatccct ttccgatacg ctggaagagg tgcttagctc atctggcgag aagtcgaaag
     4321 aagagttgag taagattcgt agcaaagcgg agcaggcact gaaacagagc cgttatcgcc
     4381 tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt cgcggcggcg cgtgctgatg
     4441 agtatgtgcg tgaaaatccg tggaccggcg tgggaattgg cgctgcaatc ggtgtagtgc
     4501 tcggcgttct gctgtcgcgt cgttaattat ggcggacact catcacgcgc aagggcccgg
     4561 taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc atggttgaaa tggtggaaac
     4621 acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa gctaatctct ttcaactttt
     4681 actgatgctg ggcctgacga tgcttttcgc tgcatttggt cttatgagcc tgatggtgct
     4741 aattatttgg gcggttgatc cgcaatatcg cctgaatgcg atgattgcca ccaccgtggt
     4801 gttgctgcta ctggcactga ttggcggtat ctggacgtta cgtaaatcgc gtaaatccac
     4861 cttgctgcgc catacacgcc atgagctggc aaacgatcgg caactgctcg aggaggagtc
     4921 ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca actgcttagc cagatccagc
     4981 aacaacggct ggatctttcc gccagtcgtc gtgaatggct ggaggcaaca ggcgcttacg
     5041 atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc gctggttggc agtagcgtga
     5101 tggcgatctg gacgattcgc catcctaata tgctggtccg ctgggccaga cgcggttttg
     5161 gcgtatggag cgcctggcgt ctggttaaaa cgaccctcaa gcagcaacag cttcgcggtt
     5221 aacttccctc tggccggagc cattccggcc ctatcactca aattttttga agatttttga
     5281 cagttttcct tgctaacaat catcattcac cacgtttatg attctctcca tcgacagcaa
     5341 cgacgctaat accgcgccct tgcacaaaaa aacaatcagc agcctgagtg gcgcagtgga
     5401 gagtatgatg aaaaaattag aagatgttgg tgtactggta gcgcgcattt taatgccgat
     5461 tctgtttatt accgcaggct ggggaaaaat caccgcttac tctggtaccc aacaatatat
     5521 ggaagcaatg ggtgtcccgg gttttatgct gccgctggtg attctgctcg agttcggtgg
     5581 tggtctggca atcctgttcg gtttcctgac tcgcaccaca gccctgttta ctgcgggctt
     5641 tacgctgctg acggcatttt tatttcacag caagtttgct gaaggcgtca actcgctgat
     5701 gttcatgaaa aacctgacaa tttctggcgg attcctgctg ctggcaatta ccggtccggg
     5761 cgcgtatagc atcgaccgcc tgctgaataa aaagtggtaa gcacgcgcta tacttaacga
     5821 taaaaaacga ggaggaagct cctcgttttt gctattggag gagagaaaaa tggggcaact
     5881 gattgacggc gtctggcatg acacctggcc ccactggcac aggcggtttt atcgcagaga
     5941 aagatcgtta tcatctctat gtttcactcg cctgcccgtg ggcgcaccgc acgctgatca
     6001 tgcgcaaact caaaggactg gaaccgttta tttccgtttc cgtagtgaac ccgctgatgc
     6061 tggaaaacgg ctggaccttt gatgacagtt ttccgggagc aaccggagat acgctctatc
     6121 aacatgaatt tctgtatcag ctttatctcc acgccgatcc acactacagc ggacgagtta
     6181 ctgttcccgt gctgtgggac aaaaagaacc acaccattgt cagcaacgaa tcagcagaaa
     6241 tcatccgcat gttcaatacc gcgtttgatg cgctgggcgc aaaagcgggt gattactacc
     6301 caccagccct gcaaacgaaa attgacgaac ttaacggctg gatttatgac accgttaaca
     6361 acggcgtgta taaagctggt tttgccacca gccagcaagc ctacgacgag gcggtggcga
     6421 aagtgtttga atcgctggcg cgactggagc agattttagg ccagcatcgt tacctgaccg
     6481 gcaaccagct aaccgaagcc gatattcgcc tgtggactac gctggtgcgt tttgatccag
     6541 tgtatgtgac ccacttcaag tgtgataagc accgcatcag cgattacctg aatctgtatg
     6601 gcttcctgcg cgatatctac cagatgccgg ggatcgccga aactgtcaat ttcgatcaca
     6661 tccgtaatca ttacttccgc agccataaga ccatcaaccc tacggggatt atttcaattg
     6721 gtccgtggca ggatctcgat gaaccgcatg gacgagatgt tcgcttcggt taaaaaaagg
     6781 caccttacga ggtgcctctg ataattagct aagtgcatgt tgttcatgtc ggatgcggcg
     6841 tgaacgcctt atcaaaccta caaaacgttt atattcaata tgttaaaatg accgcgtagg
     6901 ccagataagc gtagcgcatc tggcgatttt gcgcttgtca tcagtcttag cgcaccttca
     6961 cggcacttta ttaattcctt gaacgaatat ttactgccat ttcattcacg tctattctta
     7021 atttgctgct ttaaaaacaa gtgattgagc aaaattgagg caaaaatgga ctggtatctg
     7081 aaagtactaa aaaattatgt cggtttccgg ggtcgtgcgc ggcgcaaaga gtactggatg
     7141 tttattctgg tcaacatcat ctttacgttc gtgctggggc tgctggataa aatgttaggc
     7201 tggcaacgcg ccggtggcga aggcatcctg acgacaatct acggtattct ggtgttttta
     7261 ccgtggtggg cggttcagtt ccgccgcctg cacgacaccg accgctcggc gtggtgggca
     7321 ctgttgttct taatcccgtt tatcggctgg cttattatca tcgtctttaa ctgccaggcg
     7381 ggtacgccag gcgaaaaccg ctttggacca gatccgaaac tggaacctta aaacaaatct
     7441 gctcgccgga tgtctgactc atccggcttt tatcatcatg ttgatgaatg aataatttac
     7501 taatgtttat ttaaaatatt tcaatgtcgt tatataaatt acctataaaa aacaaccatg
     7561 tatttatctt aaatatcaaa acctttataa catccgaaaa agaaagatac gaatagcatt
     7621 tacacaatac ccggaataga ctttcgtcac ttttaattaa agggatgttt ttatgcagtg
     7681 gtatttagcc gtattaaaaa attatgttgg tttctctggt cgcgcacgtc gtaaagagta
     7741 ctggatgttt actctgatta acgccatcgt cggcgctatt atcaatgtca ttcaattgat
     7801 tttaggttta gagtttccat tcctttctct catttacctg gcagcaacaa tcattccggt
     7861 cattgctctg tgtgttcgtc gtctgcacga tactgaccgt tcaggtgtgt gggcgcttct
     7921 ctatctggta ccgatcattg gttggttagt tctgtttgta tttgcctgtt tagaaggcaa
     7981 ttctggcagc aatcgttacg gaaacgatcc taagtttggt tcaaattaat cttagaattg
     8041 gggcgatatt tcgccccttt ttattaacaa taataaatat tattttccgc taaaaagttt
     8101 gggaatttcc cgcagacacc aggatttcgc ttcccccata ctgtcacgac gccaggccat
     8161 aataatatcg atctcgctgg tcgattccgg gctgacgaca cgcaaccgcc cttccgcaat
     8221 atctttttcg accatcggat acggcatcgt cgccacgcca agccccgcca gtaatgcctg
     8281 acgtttatct tcaatcgtgc tcaccgttaa gcgcggctgt ttgtccagca gctgtacggt
     8341 caacaccggg cgctcacgag cggtatccgc taccgcaatt ccacgatatt tcacgcgcgt
     8401 cacttcagat aacggttccg gctcctgatg aatcgggtga tcaggcgcgg caacgtagac
     8461 gttcattaag gtatagagct tgcgcgagtt gatctccgac gaggaacgaa aatgcatatc
     8521 cggcgcgata acgatatccg cccgcccctg ctccagtcgt tcccacgccc ccgccagcac
     8581 ttcggtgatg attgccagtt gggtattggc ttttgccgcc agtttgtcga ttaacgggaa
     8641 aaaggcaggt gtcggtacca gcgcctcggt cacaatggtg agatgcgttt cccaaccgcg
     8701 cgcgagggct tccgcatcgg tagtcagttt atctgctgct tccagcaaaa cgcgccctcg
     8761 ctccagcaac atcctcccga cattggtgaa tttggtgcga tggcccgagc ggtcaaacag
     8821 caccacatcc agctcttctt ccagtttttg catggtgtag ctaagtgcgg aaggcacgcg
     8881 tcccagctca tccgccgccg ccgcaaaact gccccggcga tcgatcgcat ccataacccg
     8941 tagtgcttcc agcgttaatg ccctttcttt ggccatttcg ttctcattca ggaaatttga
     9001 acataccggg cagaatatct ggctaacaat gtagcgtcca gccccttacc ataaaaggaa
     9061 gtaaagagag gtcaagaatt atgattacta cccgaactgc caggcagtgt ggacaagcag
     9121 actacggatg gttgcaggcc cggtatactt tttcctttgg acactacttc gacccgaaat
     9181 tgttaggcta tgcctccctg cgtgtgctta accaggaagt gctggcccca ggtgccgcct
     9241 ttcagccgcg aacctatccc aaagtcgata ttttaaatgt gattctggat ggagaagcag
     9301 agtatcgcga cagcgaaggc aatcatgttc aggccagcgc cggtgaggcg ttgctgctct
     9361 ctacccagcc gggtgtcagc tacagcgaac acaatctcag caaagacaaa ccgttaacac
     9421 gaatgcagct ttggctggac gcctgcccgc agcgagagaa tccgctgatt caaaaactgg
     9481 cgcttaatat gggcaagcag caattaatcg cctcgccaga gggtacgatg ggaagcctgc
     9541 aattacgcca gcaagtgtgg ctgcaccata tcgtgctcga caaaggcgaa agtgcgaatt
     9601 tccagttgca tgggccacgc gcgtatttgc aatcgattca cgggaaattt catgcgctta
     9661 cgcatcatga agagaaagcg gcgctgacct gcggtgatgg ggcgtttatt cgtgacgagg
     9721 ctaacattac gctggttgcc gattccccac tgcgcgcttt gctgatagat ttgcctgtct
     9781 agttgttttt acagggagat gatgatgagt aaaaaatcgg ccaaaaagcg ccagccagtg
     9841 aagcccgtgg tggcgaaaga acctgctcgc accgccaaaa attttggcta tgaagagatg
     9901 ttgagcgagc tggaagccat cgtcgcggat gctgaaacgc gtttagccga ggatgaagct
     9961 accgcgtaaa gacaggttgc cggatgcggc gaaaacgcct tatccggcct acaaaatcca
    10021 acaacattaa cgaattaaac aacccgtagg ccggataagg tgcgccagca tcgcatccgg
    10081 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
    10141 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
    10201 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
    10261 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
    10321 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
    10381 cataacgccc atcctccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
    10441 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
    10501 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
    10561 ccacaggcat tgttgcggtg atcccctggt tacccgagcc ggagttactc atagccggaa
    10621 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
    10681 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
    10741 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
    10801 gaataaagcg gatcgccgca aacgggactt cattgacgaa cttcaggatc tcagccagcg
    10861 tcgttctgga aagcaccgtc agcggagact cttgctcgcc ctctgccaca cacgcctgct
    10921 gggtaaacac cacactattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
    10981 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
    11041 tgcaaggttc ctggatctta acggagactt tccccgccgc cagcagtgct ttggcatcgg
    11101 caattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
    11161 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
    11221 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
    11281 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
    11341 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
    11401 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaacg
    11461 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
    11521 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
    11581 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
    11641 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
    11701 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
    11761 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    11821 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    11881 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    11941 cgtagaaaaa gacggtgaca aataaaatgc caaacgcgat attcattgcc cgcaatgctt
    12001 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    12061 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    12121 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    12181 acacgcccag cgcagcgact accagcagct tggtcagcac catgccggtc gaaattttga
    12241 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    12301 aaaagggact gtctgacagc accccttccg tcacgccgaa ggtatgcaga taggaagcac
    12361 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    12421 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    12481 ctttacattc tgtgtgattc tgccagcgta ttaataaaca accgctgaaa cagatacatt
    12541 gccgggtaac caatcaccga t
//
LOCUS       DAJTOR010000119        12490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTOR010000119 DAJTOR010000000
VERSION     DAJTOR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.119"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..448)
                     /gene="rarD"
                     /locus_tag="OO249_002933"
     CDS             complement(<1..448)
                     /gene="rarD"
                     /locus_tag="OO249_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5637157.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGS"
     gene            complement(500..973)
                     /locus_tag="OO249_002934"
     CDS             complement(500..973)
                     /locus_tag="OO249_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5637158.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATATYMVG"
     gene            1138..2007
                     /gene="pldA"
                     /locus_tag="OO249_002935"
     CDS             1138..2007
                     /gene="pldA"
                     /locus_tag="OO249_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5637159.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2140..3969
                     /gene="recQ"
                     /locus_tag="OO249_002936"
     CDS             2140..3969
                     /gene="recQ"
                     /locus_tag="OO249_002936"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5637160.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMCKLERFGKPFMALIRAHVDGDDEE"
     gene            4033..4653
                     /gene="rhtC"
                     /locus_tag="OO249_002937"
     CDS             4033..4653
                     /gene="rhtC"
                     /locus_tag="OO249_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5637161.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(4715..5335)
                     /gene="rhtB"
                     /locus_tag="OO249_002938"
     CDS             complement(4715..5335)
                     /gene="rhtB"
                     /locus_tag="OO249_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5637162.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5446..6468
                     /gene="pldB"
                     /locus_tag="OO249_002939"
     CDS             5446..6468
                     /gene="pldB"
                     /locus_tag="OO249_002939"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5637163.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6476..7276
                     /gene="yigL"
                     /locus_tag="OO249_002940"
     CDS             6476..7276
                     /gene="yigL"
                     /locus_tag="OO249_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5637164.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7352..8251
                     /gene="bioP"
                     /locus_tag="OO249_002941"
     CDS             7352..8251
                     /gene="bioP"
                     /locus_tag="OO249_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5637165.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTLITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8139..9092)
                     /gene="metR"
                     /locus_tag="OO249_002942"
     CDS             complement(8139..9092)
                     /gene="metR"
                     /locus_tag="OO249_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5637166.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9328..11589
                     /gene="metE"
                     /locus_tag="OO249_002943"
     CDS             9328..11589
                     /gene="metE"
                     /locus_tag="OO249_002943"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5637167.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(11874..12119)
                     /locus_tag="OO249_002944"
     CDS             complement(11874..12119)
                     /locus_tag="OO249_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5637168.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12109..>12490)
                     /locus_tag="OO249_002945"
     CDS             complement(12109..>12490)
                     /locus_tag="OO249_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637169.1"
                     /translation="PFNPDLAGGPIENLTTDGVTINREGIAIVEKHIARFGHDPVNEV
                     MINRLKDIEKGKIPPEQVDLNFYTHECREYQRYCNLGWETGQPDGDAGYALWNHTHTA
                     TLEDYKLKGELNDLYHQDALDYDN"
BASE COUNT         2928 a         3143 c         3340 g         3079 t
ORIGIN      
        1 gcgaaccaaa agtccacaac tgaaccaaca cgccacatat cgccagaatc accgccagcc
       61 attgcatccg gcggaatcgc tcgccgagga aaatcatccc cagcacaatg ttcaccagcg
      121 ggttaataaa gtaaccaagg ctcgcttcca gcatatggtg attgttcacc gcccagataa
      181 acagtagcca gttgccacca atcagcacgg cagagactgc cagcataaaa attttctgtg
      241 gcgtctgaat cagcgttttt aaataggacc actggcggca aatgctcatc agcaccacca
      301 taaagaaaaa cgaccagatc acgcgatgcg tcaggatctc atcggcgggc acgtagtaaa
      361 tcagcttgaa gtatgctggc gcaatacccc aaataaaata agcggcaaga gcgagtaata
      421 cgccctgccg cgtttgtttt gcatccatcg ggaatactca tttttaattg gtaacagcag
      481 tttacctgct tttatgtctt caacctacca tataggtggc ggtggcggtg gcactggcaa
      541 tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac
      601 gcaacaggct actggtagca gtaaaacgct cgcctctgcc tgggcgcaga taatcaacgc
      661 gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt
      721 cgtggcgggt taaggtactt cccacgcaca ccagaccggc ggcgacatcc agcgccgacg
      781 caatgactcc gccgtgcaaa atgctttgcg cccagttacc caccatcatc ggctgatttt
      841 taaaggccag ctgtgcgaac tctttttcgt aacgctccag ctccatccct aacgcgcggt
      901 taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca
      961 gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgcttc
     1021 tttgttgttg gtttctactt taggaaggga taactaacgg caatggagtt aagtatgtaa
     1081 aatagcccgc agaaaaacat tcaccttatc aataattcgt tacggagaac acgaccgatg
     1141 cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa
     1201 gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat
     1261 atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatc
     1321 tacacccaaa ccagcgatct gaacaaagaa gcgattgcca gttacgactg ggcggaaaat
     1381 gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccactgtg gcgtgggatt
     1441 ttaggcccga actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc
     1501 aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt
     1561 tttgccaccg attaccgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac
     1621 cacgactcta acgggcgttc cgacccgacc tcccgcagct ggaaccgcct ttatactcgc
     1681 ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat
     1741 actgacgata acccggatat caccaaatat atgggttact accagcttaa aatcggctat
     1801 cacctcggtg atgcggtgtt gagtgcgaaa ggacagtaca actggaatac cggttacggc
     1861 ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt
     1921 tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgta
     1981 ggagttatgc taaacgactt gttttgatga acggttgagt ggttggcaaa tctggaaccc
     2041 agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga
     2101 aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg
     2161 aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc
     2221 cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct ggtcgtcatg
     2281 cccaccggtg gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt
     2341 accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca actgcaagcc
     2401 aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgagcagca acttgaagtg
     2461 atgacaggct gccgcaccgg gcaaattcgt ctgctttata tcgccccgga acgcctgatg
     2521 ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc cgttgatgaa
     2581 gcgcactgta tctcccaatg gggccacgat ttccgcccgg aatatgccgc gcttggtcag
     2641 ttacgccagc gtttcccgac cctgccgttt atggcgctga ctgccacagc cgacgacacc
     2701 acgcgccagg atatcgtgcg cttgctgggg ctgaacgatc cgctgattca aatcagcagt
     2761 tttgaccgtc cgaatattcg ctacatgctg atggagaagt tcaaaccgct cgatcagttg
     2821 atgcgctacg tgcaggaaca gcgcggtaag tcaggcatta tctactgcaa cagccgcgcg
     2881 aaagtagaag acaccgctgc gcgcctgcaa agcaagggaa ttagcgcggc ggcctatcat
     2941 gctgggctgg aaaataatgt tcgcgccgac gtgcaggaaa aatttcagcg cgatgacctg
     3001 caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc
     3061 gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc
     3121 gggcgtgatg gcctgcccgc ggaagcgatg ctgttttacg atccggccga tatggcgtgg
     3181 ctgcgccgtt gcctggaaga gaagccgcag gggcagttac aggatatcga acgtcataaa
     3241 ctcaatgcga tgggcgcttt tgccgaagcg caaacttgcc gtcgtctggt attgctgaac
     3301 tattttggcg aagggcggca agagccgtgc gggaactgcg atatctgcct cgatccgccg
     3361 aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat
     3421 cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc
     3481 cgcgactatg gtcatgacaa actgaaagtc tatggcatgg gccgtgataa aagccatgaa
     3541 cattgggtga gcgtgatccg ccagctgatt cacctcggcc tggtgacgca aaatattgcc
     3601 cagcattctg ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct
     3661 ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc
     3721 ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc
     3781 gatgaaagta atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct
     3841 gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgtgcaag
     3901 ctggaacgct ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac
     3961 gaagagtagt cagcagcata aaaaagtgcc aggatgaaga ctccgtaaac gtttcccccg
     4021 cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt
     4081 atgagccccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa
     4141 gaagcgatga tgggcgtgct gggtattacc tgcggcgtta tggtttgggc agggattgcg
     4201 ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct gattatggtt
     4261 ggcggtggcc tgtatctctg ctggatgggt taccagatgc tacgcggtgc actgaaaaaa
     4321 gaggctgtat ctgcgcctgc accacagatt gagctggcga aaagcgggcg cagtttcctg
     4381 aaaggtttac tgaccaatct cgctaatccg aaagcgatta tctactttgg ctcggtgttc
     4441 tcattgtttg tcggtgataa cgttggcact accgcgcgct ggggcatttt tgcgctgatc
     4501 attgtcgaaa cactggcgtg gtttaccgtc gttgccagcc tgtttgccct gccgcaaatg
     4561 cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc gttatttgcc
     4621 ggatttggca ttcatttgat tatttcgcgg tgatgccaga cgcgtcttca gagtaagtcg
     4681 gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc gatgctaaca
     4741 gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc atctgctttg
     4801 gtcctttaat ccatagagca atccgttgag caagggtggc gtaaccgatc atcacaataa
     4861 tatcgactac aatagtggtg acgccgagca cgatatactg catcagttgc ggctgctgtg
     4921 gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga ttggtgagat
     4981 tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggca agcgatttaa
     5041 ggtcaattgc accagcggcg cgccactgct ggattcccag ccaaatcaag taagccgcgc
     5101 ctgcccactt caacacttca aacgcaatca ctgagcggga aaatagcgtc cccaaaccca
     5161 cgccaaccag cacaatatga atcgccagtc cggtctgaag cccagcaata gacgccaccg
     5221 cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca gagcctggcg
     5281 acaggcttaa aatgatcgat gtcagcaggt aggcaaacca ccattctaag gtcatgatga
     5341 actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca
     5401 cgctaaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa
     5461 aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc gctgactgat
     5521 ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc ggtgcgcttt
     5581 gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg gcgtattgag
     5641 agctacgtaa aatatgcgga actggcctat gacctgttcc atttggggtt tgatgtctta
     5701 atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg
     5761 catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca gcaggaggtc
     5821 cagcccggtc cgtggcgtaa acgctatata ctggcacatt cgatgggcgg tgcgatctcc
     5881 acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg
     5941 tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa ctgggccgaa
     6001 gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg cgcgttgccg
     6061 tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt acgcttctat
     6121 gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg cgaaagtatt
     6181 ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgccaac gcttctcttg
     6241 caggctgaag aggaacgcgt ggtggataac cgcatgcatg accgtttttg tgaactccgc
     6301 accgccgcgg gccatcctgt cgaaggagga cggccgttgg taattaaagg tgcttaccat
     6361 gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc tccacgccat cgttgatttt
     6421 ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat ttcttatgta
     6481 ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata cgttatcccc
     6541 ttacgccaaa gaaactctga agctgctcac cgcgcgcggc atcaactttg tgtttgcgac
     6601 cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatta agtcttacat
     6661 gattacctcc aatggtgcgc gcgttcacga tctggatggt aatctgattt ttgctcataa
     6721 cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc cggacatcat
     6781 tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg aagagatgcg
     6841 cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag cctggattac tggagccgga
     6901 aggcgtcagc aaagtgttct tcacctgcga ttcccatgaa caactgctgc cgctggagca
     6961 ggcgattaac gctcgttggg gcgatcgcgt caacgtcagt ttctctacct taacctgtct
     7021 ggaagtgatg gcgggcggcg tttcaaaagg ccatgcgctg gaagcggtgg cgaagaaact
     7081 gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgacg ccgaaatgct
     7141 gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga aagaccttca
     7201 tcccgagctg gaagtgattg gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa
     7261 actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac ttcgctacaa
     7321 tggatactcg ttaatcaaag agttttccat tgtggcgcta cttatcatca ccacgattct
     7381 gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg atagctattt
     7441 tgcggtgctg gtgcgcgttg gcctggcggc actcgttttt ctgccgtttc tgcgtacccg
     7501 tggcaatagc ctgaaaacag tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt
     7561 gatgtatatg ctgagtttcc gcgcttatct ctatctgacg gtttccgaac tgctgctgtt
     7621 caccgtactg acgccgctct acatcacgct gatttatgac atcatgaata agcgccgtct
     7681 gcgctgggga tatgctttta gtgccttgtt ggcggtgatt ggtgccggga ttattcgcta
     7741 tgatcaggtc accgaccatt tctggactgg cttgttgctg gtgcaactct ccaatatcac
     7801 ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga tgccgcagca
     7861 taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg catggttctt
     7921 gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc tggtgtttct
     7981 tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcga cgcaggtgga
     8041 cgccggaacg ctgggaatta tgaataatat gcacgttccg gcagggctgc tggtaaacct
     8101 ggctatctgg caccaacagc cgcactggcc aacgttgatt acaggcgcgc tggtgatcct
     8161 ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga
     8221 tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg taactggctg acgctgctcg
     8281 ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt tttggtcacc
     8341 accagaccct ggcgctcaaa actctctact acccaatgcg gtagcgcggc aatacccatc
     8401 cgcgcggcaa ccatctgaat caacaataag gtgttatcga cgcttttcag tgacggactg
     8461 acgcctgccg gctgaagaaa atgccgccag acatccagtc gactacgctg caccggataa
     8521 attaatagcg tctcgctggc gagatcttcc ggtgtaatac gcgtttttgc cgccagtgga
     8581 tggtcaggtg ctaacaccag acgcacttca tagtcgaaca tcggcgaata atgcagacca
     8641 ctgcgcggca gaatatcgga cgtcattacc agatccagct ctccctgttg caaggcgggc
     8701 tgcgggtcaa atgtcacgcc cgatttaaag tccatctcga cctgcggcca gttcttatgg
     8761 aaattttcta acgcgggcgt cagccactgt atacagctat ggcactcaat ggcaatgcgc
     8821 agacgcgtct gctgcggttc attgcaggct tgcagtgcct ggctaatttg tggcagcacc
     8881 tggtttgcca gttgcaacag gatttctccc tgcggtgtaa agcgaagcgg ctggctttta
     8941 cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg agacagggcg
     9001 gattgcgttt gatgcaacgt cgccgcagcg gctgcgagtg agccgcagtt ccgcaacgct
     9061 tgtagcgttt tcagatgttt tacttcgatc atgaaagtcc ttcacttcgg catgaataat
     9121 ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc tggacggcta
     9181 aaatccttcg tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc cggtcactta
     9241 cttcagtaag ctcccgggga tgaataaact tgccgccttc cctaaattca aaatccatag
     9301 gatttacata taattagagg aagaaaaatg acaatattga atcacaccct cggtttccct
     9361 cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaga gttattgggc agggaactcc
     9421 acgcgtgaag aactgctggc ggtagggcgt gaattgcgtg ctcgtcactg ggatcaacaa
     9481 aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgtactg
     9541 accaccagtc tgctgctggg taacgttccg gcgcgtcatc agaacaaaga tggttcggta
     9601 gatattgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg
     9661 gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc ggaattcgtt
     9721 aaaggccaac agttcaaact gacctggacg cagctgctgg aggaagtgga cgaggcgctg
     9781 gcgctgggcc acaacgttaa gcctgtgctg ctggggccgg ttacctggct gtggctgggg
     9841 aaagtgaaag gtgaacagtt tgaccgcctg agcctgctga acgacattct gccggtttat
     9901 cagcaagtgc tggcagaact ggagaaacgc ggcatcgagt gggtacagat tgatgaaccc
     9961 gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg
    10021 ctccagggac aggtgaaact gctgctgact acctattttg aaggcgttac gccaaatctc
    10081 gacacgatta ctgcgctgcc tgttcaggga ctgcatgtgg accttgtaca tggtaaagat
    10141 ggcgttgctg aactgcacaa gcgcctgcct tctgactggc tgctgtctgc gggactgatc
    10201 aatggtcgta acgtctggcg cgccgatctt actgagaaat atgcgcaaat taaggacatt
    10261 gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac
    10321 ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccttacaa
    10381 aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg
    10441 gcagagtgga gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca taatccggcg
    10501 gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa
    10561 gtgcgtgctg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt
    10621 ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat
    10681 ctcgacgcca acaactaccg cacgggcatt gcggaacata tcaagcaggc cattgttgag
    10741 caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg
    10801 gaatactttg gcgagcacct cgacggattt gtctttacgc aaaacggttg ggtacagagc
    10861 tacggttccc gctgcgtgaa gccaccgatt gttattggtg acgttagtcg cccggcaccg
    10921 attaccgtgg agtgggcgaa gtatgcgcaa tctctgactg ataaaccggt gaaagggatg
    10981 ctgactggcc cggtgaccat tctctgctgg tcgttcccgc gtgaagatgt cagccgtgaa
    11041 accatcgcca aacaaattgc gctggcgctg cgtgatgaag tggcggacct ggaagccgca
    11101 ggaattggca tcatccagat tgatgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt
    11161 agcgactggg atgcatatct ccagtggggc gtggaggcct tccgtatcaa cgccgccgtg
    11221 gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg
    11281 gattccattg cggcactgga tgcagacgtc atcaccatcg aaacctcgcg ttccgacatg
    11341 gagttgctgg agtcgtttga agagtttgat tatccgaatg aaatcggtcc aggcgtctat
    11401 gacattcact cgccaaacgt accgagcgta gaatggattg aagccttgct gaagaaagcg
    11461 gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc
    11521 ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaatttgcgt
    11581 cggggatgat gaatattaac gagtcactgt aaattgcagt acgatgaaag aggcaaaact
    11641 gcctgatacc aggcctacat aatttatgca atttattgaa tttttgagtt ttgttggccg
    11701 gacaaggcgt tcacgccgca tccggcatga acaacgcgca ctttgccaat aatctgatgg
    11761 cttccatcac cggaagccat tttgctatct gcgcccgttc agatacagaa ataaccgaat
    11821 catttttacg caaaatataa tcctgagcga tttgttcagt aatcaacgct acgttattta
    11881 ttctcacttt tcacgaaatt gtatcccttt tcagtaacgt ggtagtgagg catagactta
    11941 tgtccgctat cctcaataat aaccaaaccc tcgttagcta atttgttaac aatgttaaca
    12001 acattaccga atcctggaat ttgccttatg gatagtgtgc gatcgagatt catccagttc
    12061 cagtttatat ctttctcagc cataactttc agtacgttaa tttcagtttc agttatcata
    12121 gtcaagtgca tcctggtggt ataagtcatt aagttctccc ttaagtttat aatcttctag
    12181 agtagcagta tgcgtatgat tccagagtgc atagcctgcg tcaccatcag gttgtcctgt
    12241 ttcccaacca agattacagt aacgctgata ttcccgacac tcatgagtat agaagttaag
    12301 atctacttgt tctgggggga tttttccttt ctcaatgtct ttcagtctat tgatcatcac
    12361 ttcatttaca gggtcatgac caaatctggc tatgtgtttt tcaacaatag cgataccttc
    12421 cctgttgatt gttacgccat ctgtcgtcag attttctatt ggaccaccgg caaggtcggg
    12481 attaaatggt
//
LOCUS       DAJTOR010000120        12468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTOR010000120 DAJTOR010000000
VERSION     DAJTOR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.120"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..770)
                     /gene="ychF"
                     /locus_tag="OO249_002946"
     CDS             complement(<1..770)
                     /gene="ychF"
                     /locus_tag="OO249_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5637170.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFM"
     gene            complement(887..1471)
                     /gene="pth"
                     /locus_tag="OO249_002947"
     CDS             complement(887..1471)
                     /gene="pth"
                     /locus_tag="OO249_002947"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5637171.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            1749..2027
                     /gene="ychH"
                     /locus_tag="OO249_002948"
     CDS             1749..2027
                     /gene="ychH"
                     /locus_tag="OO249_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5637172.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2082..3761)
                     /gene="dauA"
                     /locus_tag="OO249_002949"
     CDS             complement(2082..3761)
                     /gene="dauA"
                     /locus_tag="OO249_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5637173.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVIIYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPISGRLAFFPNRRAAMADL"
     gene            complement(3886..4833)
                     /gene="prs"
                     /locus_tag="OO249_002950"
     CDS             complement(3886..4833)
                     /gene="prs"
                     /locus_tag="OO249_002950"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5637174.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(4984..5835)
                     /gene="ispE"
                     /locus_tag="OO249_002951"
     CDS             complement(4984..5835)
                     /gene="ispE"
                     /locus_tag="OO249_002951"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5637175.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(5835..6458)
                     /gene="lolB"
                     /locus_tag="OO249_002952"
     CDS             complement(5835..6458)
                     /gene="lolB"
                     /locus_tag="OO249_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5637176.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            6672..7928
                     /gene="hemA"
                     /locus_tag="OO249_002953"
     CDS             6672..7928
                     /gene="hemA"
                     /locus_tag="OO249_002953"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5637177.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7970..9052
                     /gene="prfA"
                     /locus_tag="OO249_002954"
     CDS             7970..9052
                     /gene="prfA"
                     /locus_tag="OO249_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5637178.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9052..9885
                     /gene="prmC"
                     /locus_tag="OO249_002955"
     CDS             9052..9885
                     /gene="prmC"
                     /locus_tag="OO249_002955"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5637179.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            9882..10274
                     /locus_tag="OO249_002956"
     CDS             9882..10274
                     /locus_tag="OO249_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5637180.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10278..11087
                     /locus_tag="OO249_002957"
     CDS             10278..11087
                     /locus_tag="OO249_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5637181.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11123..11977
                     /gene="kdsA"
                     /locus_tag="OO249_002958"
     CDS             11123..11977
                     /gene="kdsA"
                     /locus_tag="OO249_002958"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5637182.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12126..12233)
                     /locus_tag="OO249_002959"
     CDS             complement(12126..12233)
                     /locus_tag="OO249_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5637183.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3096 a         3177 c         3249 g         2946 t
ORIGIN      
        1 tgcataaact cgtcacgttc ttcgtcgtcc agttcggcaa tgtctgcttc aacagcagca
       61 caaaccggaa ccacaacaga accttctttc gccgcgattt cacgcacctg gtcaagatat
      121 gggttgtttt caaaaccgtc ttcgttgacg ttggcgatgt acattgttgg tttcagcgtc
      181 aggaagctca ggtaacgaat agccgctttc tcttcagcgc ttaaatccag cgcgcgcagc
      241 atacctgcat tttccaactg gggcaggcat ttttccagga ccgccagctc agctttcgcg
      301 tctttatcgc cacctttggc tttcttctgt acgcgatgaa tcgcacgttc gcaggtgtcg
      361 aggtctgcca gcgccagttc ggtgttgata acttcaatat cgtcagccgg gttaactttg
      421 ccggaaacgt gaatgatgtt gtcattttca aagcagcgaa caacgtgacc gatcgcttcg
      481 gtttcacgga tgttggtcag gaactggtta cccagacctt cgcctttcga tgcgcctttt
      541 accagaccgg cgatatcgac aaattccatg gtcgtgggaa gcgtacgctg cggttttacg
      601 atttcagcca gttgatccag gcgaggatca ggcattggta cgacgcctgt gttcggctca
      661 atggtgcaga atggaaagtt ggccgcttca ataccggctt tggtcagcgc gttgaacagg
      721 gtagatttcc cgacgttggg caaaccgacg ataccgcatt tgaatcccat gatttaaatc
      781 accttaatat cttaataatc aacctgttat tgctaacaga ttgcagaaat ggaaataact
      841 ttgcctatta tacacggcac tcggcaaaaa tgccgcagac aacgacttat tgcgctttaa
      901 aggcgtgcaa tcggttcgtt gctttggtca agccatctgt aaaccacatt tcagtacaac
      961 gcgccgcttc gtcaatggct tcatcaatta acttctgttc actaacaggc ggtttgccta
     1021 acacaaaacc gacaacttta tttttatcgc ccggatgacc gattccgatg cgtaaacggt
     1081 gaaagttagg gttattaccc aatttactga tgatgtcttt cagtccattg tgaccaccat
     1141 ggccaccgcc caatttaaat ttggcgacgc caggaggcag atccagttcg tcgtgggcca
     1201 ccagaatttc gtccggatta atgcggaaaa aactggccat cgccgcaacg gctttgccgc
     1261 tgagattcat aaatgtagtc gggactaaca ggcggacatc ttcgcctcca agagtgactc
     1321 gcgaagtata accaaagaat ttagcctctt cgcgcagcgg agcgcgcaaa cgctctgcca
     1381 gtaagtcaac gaaccaggca ccagcattat gtcgcgttgc ggcgtattca gcaccggggt
     1441 tcgccaggcc gacaatcaat ttaatcgtca cgtttttttg tcctgagtgt gtacataact
     1501 ggcgcgtagt ttactggttg cgaccccgct tgacaaaaaa ctgcgtatca aatgcagata
     1561 acgtaataat tgcctgagtg ggctattaga aagtcaaggt gttcaggcgt ttatttgtaa
     1621 agttttgttg aaataagggt tgtaattgtg atcacgcccg cacataaccc acagggtgtt
     1681 gtctatactt tacacataag gaagaggggt attccctgtt acaacccaga aagttccgga
     1741 ggtgacatat gaaacgcaaa aacgcttcgt tactcggtaa cgtgctcatg gggttgggtc
     1801 tggtggtaat ggtggtcggc gtggggtatt caatcctcaa ccagttacca cagtttaata
     1861 tgccccagta tttcgcacat ggtgcagtgc taagtatttt cgtcggtgcc attctctggc
     1921 tggcgggtgc ccgtgttggc gggcatgaac aggtgtgcga ccgttactgg tgggttcgcc
     1981 actatgacaa acgttgccgc cgtagcgata atcgccgtca tagctaacaa ataatgcagt
     2041 ttgctgacca gtcagtttgc gctgactggt caatctcgta cttataaatc cgccatcgcc
     2101 gcgcgacgat tcgggaagaa cgcgaggcgt cccgagattg gttgaatgcc tgcgcgagcc
     2161 atagtgcgca gtggctggaa ttccacgttg cacacgcgca gttcacatcc ttcgggcaga
     2221 cgcttcacaa aacgctggaa cgcatcaaga ccaccagcat caagtaccgg aacggcatcc
     2281 cacttcagaa tcacaatccg tttgccttca agacgtgact ccaggtccgt gaacaagcct
     2341 tcagcagcag caaaaaacag cgggccaata acgcgcagta ccagaacatc gtctggaaca
     2401 tctacgacta ccggtgccag gcgagtcata cgtgcgatac gacgcataaa cagcagcgat
     2461 gccagcacga tcccgacgct gatggcaata accatatcaa acagcacggt cagcgacatg
     2521 cacagcagca tgacaatgat gtcatctttc ggtgcatgac gcagcaagtc gaccacttta
     2581 tgcgcttcac tcatgttcca cgccaccatc aacagcaggg ctgccatagc ggaaagcggc
     2641 agccaggaga gcagcggtgc cagtaccagc agggcaagaa taaccagaat agagtggatc
     2701 accgctgaga taggggaagt tgccccggca cggacgttag cggcagaacg cgcgatggca
     2761 gctgtagcgg taataccacc aaagaatgga gcgataatat tccccagtcc ctgtccaacc
     2821 agttcgctgt tcgccttgtg tttcgtcccg gtcataccat ccagtaccac ggcgcagagc
     2881 agagattcga ttgcgccgag cattgccatt gagaatgccg caggcagcag tgtgcgaata
     2941 gaatcccagg ttagcgtgaa ttctgaatta ggcagatccc acggcagcac cagttgcggc
     3001 agcagttgcg gaataccgtt accctgagaa ccatcggcca ggacgtagtg gaattgcgaa
     3061 ccgatggtag caacatgtcc gccgagcagg ttaacaatcc ccatcaccgc gcaaccagcc
     3121 agcaaggccg gaaggtgacc gggtaaacga atgcccagac gcggccaaaa aacaagaata
     3181 cctagcgtca caatgccaat ggcagcatca cccacattaa tggtcggcag cgccataaat
     3241 aatgcgccga ctttttgtag ataatgttcc gggacatggg ccatttgcag accgagaaaa
     3301 tctttaatct gcatggtacc gatggtgatc ccgatacccg aggtgaaacc taaggtgacg
     3361 gaaaccggaa tatactcaat caggcgacca aagcgtgcca gacccatcag aatcaaaaag
     3421 atccccgaca gcaaggccgc aaccagcagt cctgccagtc caaactgttg cgacacggga
     3481 tagataatta ccacaaatgc cgcagtcgga ccggaaacgc taaagcgtga cccacccgtc
     3541 agagcaatga caatccccgc aacagctgcg gtatataaac cgtactgggg tgccacacca
     3601 ctaccaatag ccaacgccat cgccagcggg atagcaataa tcccgacggt tatcccggca
     3661 atcaggtcac gggtaaaccg tgcggcagta tatttttctt tccagcaagc gtcgatcaga
     3721 gcgcggaaag gcatcacatg tgaggaaaat attttgttca caataatgtt tcatccgtga
     3781 gcgcatcatc tgtcaactaa atggcaggtg aaggaggcat aggtcataca aatggatatt
     3841 acagacaaaa aaacccgccg cagcgggtct ttgagccggg ttcgattagt gttcgaacat
     3901 ggcagagatc gattcttcgt tgctgatacg acgaatcgct tcggccagca tacctgacag
     3961 ggtcagagta cgcacgttcg gcagtgattt gatttcatcg ctcagcggaa tggtatcgca
     4021 gacaacgact tcatcaatta cagagttacg caggttgttc gccgcgttgc cagagaagat
     4081 cgggtgagtc gcgtacgcaa atacacgttt agcaccacgt tctttcagag cttcagcagc
     4141 tttacacagc gtaccgccag tgtcgatcat atcatcgacc agtacgcagt cacgacctgc
     4201 aacgtcaccg atgatatgca tcacctggga aacgttcgca cgcggacgac gtttgtcgat
     4261 gattgccata tcggtatcgt tcagcagctt agcgatagcg cgggcacgca caacgccgcc
     4321 gatgtccgga gaaaccacaa ttgggttatc cagattcagc tgcagcatgt cttccagcag
     4381 gatcgggcta ccaaatacgt tatcaaccgg aacgtcgaag aaaccctgaa tctgttcagc
     4441 gtgcagatcc actgtcagca cacggtcaac accgacgctg gagaggaagt ctgcaaccac
     4501 tttcgcagtg attggtacac gagcggaacg gacgcgacgg tcctggcgcg catagccaaa
     4561 gtaggggata acagcggtga tacgacctgc ggaagcacga cgcagggcat caaccataac
     4621 gactaattcc atcaggttgt cgttagtagg ggcacaagtg gactggatga tgaaaatatc
     4681 accaccgcgt acattttcat taatttgtac gctgacttcg ccatcgctaa agcgacctac
     4741 agcggcgtcg ccgagtgaag tgtacaggcg gttggcaata cgttgtgcta gttccggggt
     4801 ggcgttacca gcaaaaagct tcatatcagg cacgagaaga acctcaggca tgcgtccatt
     4861 ggtggaaaga atctgccgaa aactgtgcgg gccaggcatg atcctttcca ggcagtgtat
     4921 taaagagcgc gatgcaacgt ctggaacaag gtgacgttgt caccgaaact cagcttgccc
     4981 ggcttaaagc atggctctgt gcaatgggga aagattaacg cctttcgcca caaagccatt
     5041 gagccattcc ggggcttgct ctagcacctg gcgggcttca gactctgtat caaattcagc
     5101 aaagacacag gcccctgtcc cagtcaggcg cgacggggcg tattctaaca gccaggaaag
     5161 caccgcatca acctcgcgaa aacgttttct tgcgataacc tcgcaatcat tgctgaattc
     5221 acattttagc aacgtttcta ttgacctttt tggcgtattg cgcgggagtt caggatcttt
     5281 aaaaatcacc ggagtcggaa tacttacacc agggtgcgcc accagatacc acttctctgg
     5341 cggatccacc ggcgttagta tttcaccaac gccttcggca aacgcggcat gcccccgaac
     5401 aaagacagga acatctgcgc ccagcgtcag ccccatttcc gccagctcat ccatgcttag
     5461 cccgcattgc cagagatgat ttaatgccac caggaccgtc gcggcattgg atgaaccacc
     5521 gccgagaccg ccgcccatcg gcaaacgctt gtcaatgctg atattcgcac cgcttcccgt
     5581 cggaagacgc ccgctgtctg ccgcagtttt catcaacaat cgcgctgcgc gaacgatcag
     5641 gttatcttca tgttccacgc cttcaacggg cgttaacaga cgaatatccc catcgtcacg
     5701 aagctcaatg ctgatggtgt cgccgtaatc aagaaactga aacagcgttt gcagcgtgtg
     5761 gtaaccatcc gcacgctgac cggtaatgta taaaaacaga ttaagttttg ccggagaggg
     5821 ccactgtgtc cgcattattt cactatccag ttatccattt ttaacttgat gcgttgacca
     5881 ccgtcggtga gttccatatt ggctggcatc gcaggatgcg ttttggtgtc ataaccacca
     5941 taaacaacct tccagttttt gccattctgg ctgtaggtaa tttcgctcag gcggtactgg
     6001 tcgtccagtt tgtagtcggt tgcatcaccc ggtaaaccta aaatccactg gcgcaagctg
     6061 ttgagcggaa ttggcattcc ggtcaatttg ccaatcatct cttcggcgtc atcggcggta
     6121 taacgctgac ctttattgtc gactaactgc acgttacccg gttgagcatt cagctccagt
     6181 tccgtgctgc ccaatgggtt agtgagcagc agacggtagc gatcctggcc ggtttgctgc
     6241 cagaaaaagc gggcgtacac tttttgttgg tcagaaatat aagcgaacgc gccgcgagtc
     6301 tgatactgat taagattgcg cacgtcttgc tgatgctgac gccattgtgg cgaatccggg
     6361 cttttgccag gacctttggg cgtggtaacg gaacaggcag tgagcacaag agcagccagc
     6421 ggtagcaggc ggataagacg aaaatcgggc aggggcatag tgatgacaag tccttgagat
     6481 acgttgcagt tataaccctt aatgctagcg ttaccgtccg ctatcgtcta tgttcaagtt
     6541 gtcttaattg ccagaatcta acggctttcg gcaattactc caaaaggggg cgctctcttt
     6601 tattgatctt acgcatcctg tatgatgcaa gcagactaac tctatcaacg ttggtattat
     6661 ttcccgcaga catgaccctt ttagcactcg gtatcaacca taaaacggca cctgtatcgc
     6721 tgcgagaacg tgtatcgttt tcgccggata agctcgatca ggcgcttgac agcctgcttg
     6781 cgcagccgat ggtgcagggc ggcgtggtgc tgtcgacgtg caaccgcacg gaactttatc
     6841 ttagcgttga agagcaggat aacctgcaag aggcgttaat ccgctggctt tgcgattatc
     6901 acaatcttaa tgaagaagat ctgcgtaaaa gcctctactg gcatcaggat aacgacgcgg
     6961 ttagccattt aatgcgtgtt gccagcggcc tggattcact ggttcttggg gagccgcaga
     7021 tcctcggtca ggttaaaaaa gcgtttgccg attcgcaaaa aggtcatatg aaggccagcg
     7081 aactggaacg catgttccag aaatctttct ctgtagcgaa acgcgttcgc actgaaacag
     7141 atatcggtgc cagtgctgtg tctgtcgctt ttgcggcttg tacgctggcg cggcagatct
     7201 ttgaatcgct ctctacggtc acagtgttgc tggtaggcgc gggcgaaacc atcgagctgg
     7261 tagcgcgtca tctgcgcgaa cataaagtac agaagatgat tatcgccaac cgcactcgcg
     7321 aacgtgccca aattctggca gatgaagttg gcgcggaagt gattgccctg agtgagatcg
     7381 acgaacgtct gcgcgaagcc gatatcatca tcagttccac cgccagcccg ttaccgatta
     7441 tcgggaaagg catggtggag cgcgcattaa aaagtcgtcg taaccaacca atgctgttgg
     7501 tggatattgc cgttccgcgc gatgttgagc cggaagttgg caaactggcg aatgcttatc
     7561 tttatagtgt ggacgatctg caaagcatca tttcgcacaa cctggcgcag cgtaaagccg
     7621 cagcggttga ggcggaaact attgtcgctc aggaaaccag cgaatttatg gcgtggctgc
     7681 gagcacaaag cgccagcgaa accattcgcg agtatcgcag ccaggcagag cacgttcgcg
     7741 atgagttaac cgccaaagcg ttagcggccc ttgagcaggg cggcgacgcg caagccatta
     7801 tgcaggatct ggcatggaaa ctgactaacc gcttgatcca tgcgccaacg aaatcacttc
     7861 aacaggccgc ccgtgacggg gataacgaac gcctgaatat tctgcgcgac agcctcgggc
     7921 tggagtagca gtacatcatt ttcttttttt acagggtgca tttacgccta tgaagccttc
     7981 tatcgttgcc aaactggaag ccctgcatga acgccatgaa gaagttcagg cgttgctggg
     8041 tgacgcgcaa actatcgccg accaggaacg ttttcgcgca ttatcacgcg aatatgcgca
     8101 gttaagtgat gtttcgcgct gttttaccga ctggcaacag gttcaggaag atatcgaaac
     8161 cgcacagatg atgctcgatg atcctgaaat gcgtgagatg gcgcaggatg aactgcgcaa
     8221 agctaaagaa aaaagcgagc aactggaaca gcaattacag gttctgttac tgccaaaaga
     8281 tcctgatgac gaacgtaacg ccttcctcga agtccgagcc ggaaccggcg gcgacgaagc
     8341 ggcgctgttc gcaggcgatc tgttccgtat gtacagccgt tacgccgaag cccgccgctg
     8401 gcgggtagaa atcatgagcg ccagcgaggg tgaacatggt ggttataaag agatcatcgc
     8461 caaaattagc ggtgatggtg tgtatggtcg tctgaaattt gaatctggcg gtcatcgcgt
     8521 gcagcgtgtt cctgctacgg aatcgcaggg tcgtattcat acttctgctt gtaccgttgc
     8581 ggtaatgcca gaactgcctg acgcagaact gccggacatc aacccagcag atttacgcat
     8641 tgatactttc cgctcgtcag gggcgggtgg tcagcacgtt aacaccaccg attcggcaat
     8701 tcgtattact cacttgccga ccgggattgt tgttgaatgt caggatgaac gttcacaaca
     8761 taaaaacaaa gcgaaggcgc tttcagtgct cggtgctcgc atccacgctg ctgaaatggc
     8821 aaaacgccaa caggccgaag cgtctacccg tcgtaacctg ctggggagtg gcgatcgcag
     8881 cgaccgtaac cgtacttaca atttcccgca ggggcgcgtt accgatcacc gcatcaacct
     8941 gacgctctac cgcctggatg aagtgatgga aggtaagctg gatatgctga ttgaaccgat
     9001 tatccaggaa catcaggccg accaactggc ggcgttgtcc gagcaggaat aatggaatat
     9061 caacactggt tacgtgaagc aataagccaa cttcaggcga gcgaaagccc gcggcgtgat
     9121 gctgaaatcc tgctggagca tgttaccggc agagggcgta cttttattct cgcctttggt
     9181 gaaacgcagc tgactgacga acaatgtcag caacttgatg cgctactgac acgtcgtcgc
     9241 gatggtgaac ccattgctca tttaaccggg gtgcgagaat tctggtcgtt gccgttattt
     9301 gtttcgccag cgaccttaat tccgcgcccg gatacggagt gtctggtgga gcaggcactg
     9361 gcgcggttgc ctgaacaacc ttgccgtatt cttgatctcg ggacgggtac cggggcgatt
     9421 gcgcttgcgc tggctagcga gcgcccggac tgcgaaatta tcgctgtaga tcgtatgcct
     9481 gatgctgtct ccctggcaca acgtaatgcc cagcatctgg cgatcaaaaa tatccacatt
     9541 ctgcaaagcg actggtttag cgcgctagcc gggcagcagt ttgcgatgat tgtcagcaat
     9601 ccgccgtata ttgacgagca ggacccacat cttcaacaag gcgatgtccg ctttgagccg
     9661 ctcactgcgc tggttgcggc agacagtgga atggcagaca tcgtgcatat catcgaacag
     9721 tcgcgtaacg cgctggtatc cggcggcttt ctgcttctgg aacatggctg gcagcagggc
     9781 gaagcggtgc gacaagcatt tatcctcgcg gggtatcatg acgtcgaaac ctgccgtgac
     9841 tatggtgata acgagcgcgt aacgctcggc cgctattatc aatgacaagt ttttctacac
     9901 tgcttagtgt tcatcttatt agtatcgcgc tttctgttgg gctattaacc ttacgtttct
     9961 ggctacgtta tcagaagcat ccacaggcat ttgctcgctg gacgcgcatt gtgccgccgg
    10021 ttgtcgatac ggtgttattg ttaagcggca ttgcgttgat ggctaaagcg cacatcctgc
    10081 cattttccgg gcaggcacag tggctgactg aaaagctgtt tggagttatc atttatatcg
    10141 ttttgggttt tattgcactc gattatcgtc gtatgcacag tcagcaggcg cgcattattg
    10201 ccttcccgct ggcgttggtg gtgctgtaca tcatcattaa actcgccacc acaaaagtac
    10261 cgttactggg gtaagtcatg agatcgttag ctgatttcga atttaataaa gcgccattgt
    10321 gcgaaggcat gatcctggct tgcgaagcaa tccgccgcga ttttccctcg caagatgttt
    10381 acgacgaact ggagcgtctc gttagtctgg cgaaggaaga aatcagccag cttctgcctt
    10441 tagaagagca gctggaaaaa ctgatcgcgc tgttttacgg cgactgggga tttaaagcct
    10501 cacgcggcgt ttatcgtctt tccgatgcat tatggctgga ccaggtgtta aagaatcgac
    10561 agggcagtgc ggtatcatta ggtgcggttt tattatgggt cgcgaatcgt ctcgatttgc
    10621 cgctgctgcc ggtgattttc cctacgcagc tgatattgcg cattgaatgt ccggatggcg
    10681 aaatttggct gattaatcct tttaacggtg aatcgttaag cgaacatatg ctggacgtat
    10741 ggttaaaggg aaatatcagc ccgtcggcgg aactgtttta tgaagacctt gatgaagctg
    10801 ataacattga ggtaatccgc aaattgctgg atacactcaa agcctcgttg atggaagaaa
    10861 atcagatgga gctggcgtta cgcaccagcg aagctttatt acaattcaac cctgaagatc
    10921 cctatgaaat tcgcgatcgc gggttgattt atgcgcaact ggattgcgaa cacgttgcgt
    10981 tgaacgattt aagttatttc gttgaacagt gtccggaaga cccgatcagc gaaatgatcc
    11041 gtgcgcaaat aaataacatc gcgcataaac atattgtgct gcattaatta atcgacattt
    11101 tactcaagat taaggcgatc ctatgaaaca aaaagtggtt agcattggcg acatcaacgt
    11161 agcaaatgac ctgccgttcg tactgtttgg cggtatgaac gtgctggaat ctcgcgatct
    11221 ggcgatgcgc atttgcgagc actacgtaac tgtgacccag aaactgggta tcccttacgt
    11281 gttcaaagcc tcttttgaca aagccaaccg ctcctccatc cactcttatc gtggaccggg
    11341 cctggaagaa gggatgaaaa tcttccaaga gttgaagcag acttttggcg tgaaaattat
    11401 caccgacgtt cacgaaccaa gtcaggcaca gcccgttgct gatgtcgtgg atgtgattca
    11461 gttgccggcg tttcttgctc gccagactga cctggttgaa gccatggcga aaaccggtgc
    11521 ggtaattaac gtcaagaaac cacagtttgt cagcccggga cagatgggta atatcgttga
    11581 taaattcaaa gaaggcggca acgaaaaagt gattctttgc gatcgtggtg ctaacttcgg
    11641 ctatgacaac ctggttgtcg atatgctggg cttcagcatt atgaagaaag tgtctggtaa
    11701 ctcgccggtg attttcgacg tgacccacgc actgcaatgc cgcgatccgt ttggcgcagc
    11761 ttccggtggt cgtcgtgctc aggtggctga gctggcacga gccggtatgg cggtaggtct
    11821 ggcggggctg tttattgaag cgcatccgga tccggaacat gcgaaatgtg atggtccatc
    11881 cgcgctgccg ctggctaaac tggaaccgtt cctcaagcag atgaaagcga ttgatgatct
    11941 ggtgaaaggt ttcgaagaac tggataccag caagtaatct tttttgcttg aaaaataaag
    12001 tcttagcgtt ctgcgttaag acttttttca tgggtgccag atacaaaaaa ggccgcaggc
    12061 tgttacccct gcggccggtt tcgggcgcat attgccatca cggcagcctg acgcccgttt
    12121 tcaccttact tccggttacg ccaccagccg acaatcgctg cggtaataat tcccgccagg
    12181 atcggtgctg ccaggtcgtg ccagaaaatc atggcaaact gcgcgagcgt catatagccg
    12241 ccttgttgta atgacaacat tttgcggcta ttcttgaatt gttctggttc aagattagcc
    12301 cccgttctat tgtcaggtta aacctctcaa cgtgcggggg ttttctcttt ccagcaacca
    12361 atgccaccag ggataaagcc cccgcaacat tgcgcctcac cggataacgc cggcttgatg
    12421 tggatactac gtctcaattc atcttcactt catccctgaa atgtttgc
//
LOCUS       DAJTOR010000121        12408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTOR010000121 DAJTOR010000000
VERSION     DAJTOR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.121"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO249_002960"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO249_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5637184.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OO249_002961"
     CDS             1946..2230
                     /locus_tag="OO249_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5637185.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3302
                     /locus_tag="OO249_002962"
                     /pseudo
     CDS             2410..3302
                     /locus_tag="OO249_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3302..5446
                     /gene="fadJ"
                     /locus_tag="OO249_002963"
     CDS             3302..5446
                     /gene="fadJ"
                     /locus_tag="OO249_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5637186.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTVQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTTLEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            5649..6134
                     /gene="sixA"
                     /locus_tag="OO249_002964"
     CDS             5649..6134
                     /gene="sixA"
                     /locus_tag="OO249_002964"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5637187.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            6809..7069
                     /locus_tag="OO249_002965"
     CDS             6809..7069
                     /locus_tag="OO249_002965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5637188.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDT"
     gene            7076..7372
                     /locus_tag="OO249_002966"
     CDS             7076..7372
                     /locus_tag="OO249_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5637189.1"
                     /translation="MTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSY
                     KSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7454..10096
                     /gene="yfcU"
                     /locus_tag="OO249_002967"
     CDS             7454..10096
                     /gene="yfcU"
                     /locus_tag="OO249_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCR5637190.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQIRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQINYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            10116..10868
                     /locus_tag="OO249_002968"
     CDS             10116..10868
                     /locus_tag="OO249_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5637191.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQVDEEQSRKG"
     gene            10884..11393
                     /locus_tag="OO249_002969"
     CDS             10884..11393
                     /locus_tag="OO249_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5637192.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            11390..11878
                     /locus_tag="OO249_002970"
     CDS             11390..11878
                     /locus_tag="OO249_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5637193.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            11875..12408
                     /locus_tag="OO249_002971"
                     /pseudo
     CDS             11875..12408
                     /locus_tag="OO249_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         3141 a         2964 c         3277 g         3026 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttcgtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgttgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgttgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgttgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatggttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgcgcgat ttaatgcccg taccgcctgc agtagcagaa
     2941 tattctaccg gcttgcggat gggtgacacc gcagagcaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgcgtg gatgattacc cagacattgc atgaacttcg ccgtcgcggc ggtggatttg
     3241 gtttagttac cgcctgtgct gccggtgggc ttggcgcggc aatggttctg gaggcggaat
     3301 aatggaaatg gcatcagcgt ttactcttaa tgttcgtctg gacaacattg ctatcatcac
     3361 catcgacgta ccggatgaga aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg
     3421 cgccattatt aagcaactcc gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc
     3481 gaaaccggac aattttatcg ccggagcaga catcaacatg atcggcaact gcaaaacggt
     3541 gcaagaagcg gaagctctgg cgcggcaggg ccaacagttg atggcggaga ttcatgcttt
     3601 gcccattccg gttatcgcgg ctattcatgg cgcttgcctg ggtggtgggc tggagttggc
     3661 gctggcgtgc cacggtcgcg tttgtactga cgatcctaaa acggtgctcg gtttgcctga
     3721 agtacaactt ggattgttac ccggttcagg cggcacccag cgtttaccgc gtctgatagg
     3781 cgtcagcaca acattagaga tgatcctcac cggaaaacaa cttcgggcga aacaggcatt
     3841 aaagctgggg ctggtggatg acgttgttcc gcactccatt ttgctggaag tcgctgttga
     3901 gctggcgaag aaggatcgcc catcttcccg ccctctacct gtacgcgagc gtattctggc
     3961 ggggccgtta ggtcgtgcgc tgctgttcaa aatggtcggc aagaaaacag aacacaaaac
     4021 tcaaggcaat tatccggcga cagaacgcat cctggaggtt gttgaaacgg gattagcgca
     4081 gggcaccagc agcggttatg acgccgaagc tcgggcgttt ggcgaactgg cgatgacgcc
     4141 acaatcgcag gcgctgcgta gtatcttttt tgccagtacg gacgtgaaga aagatcccgg
     4201 tagtgatgca ccgcctgcgc cattaaacag cgtgggaatt ttaggtggtg gcttgatggg
     4261 cggcggtatt gcttatgtca ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat
     4321 caacccgcag ggcataaatc atgcgctgaa gtacagttgg gatcagctgg agggcaaagt
     4381 tcgccgtcgt catctcaaag ccagcgaacg tgacaaacag ctggcattaa tctccggaac
     4441 gacggactat cgcggctttg cccatcgcga tctgattatt gaagcggtgt ttgaaaatct
     4501 cgaattgaaa caacagatgg tggcggaagt tgagcaaaat tgcgccgctc ataccatctt
     4561 tgcttcgaat acgtcatctt taccgattgg tgatatcgcc gctcacgcca cgcgacctga
     4621 gcaagttatc ggcctgcatt tcttcagtcc ggtggaaaaa atgccgctgg tggagattat
     4681 tcctcatgcg ggtacatcag cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca
     4741 gggcaaaacg cccattgtcg tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc
     4801 gccttacatt aatgaagcta tccgcatgct gaccaaaggt gaacgggtag agcacattga
     4861 tgccgcgcta gtgaaatttg gttttccggt aggtccaatc caacttttgg atgaggtagg
     4921 aatcgacacc gggactaaaa ttattcctgt actggaagcc gcttatggag aacgttttag
     4981 cgcgcctgca aatgttgttt cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg
     5041 tcggggtttc tatctttatg gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcccgc
     5101 catttacccg ctcattggcg cacaagggca ggggcgactc tccgcaccgc aggttgctga
     5161 acggtgtgtg atgttgatgc tgaatgaagc agtacgttgt gttgatgagc aggttatccg
     5221 tagcgtgcgt gacggggata ttggtgcggt atttggcatt ggttttccgc catttctcgg
     5281 tggaccgttc cgctatatcg attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg
     5341 actggccacg cagtatggtt cccgttttac cccttgcgag cgtttggtcg agatgggcgc
     5401 gcgtggggaa agtttttgga aaacaactgc aactgacctg caataagaag gtcaaagcta
     5461 tatgaatccg cgctgaatgg cggagtgttg gtcaaaatgt aaacgcatat tgactatact
     5521 tacgccattg aggtaaaaaa cagcgtttca ttcggtgaat ggataaggca caatgccggc
     5581 caccgttttc tttctctggt ttcagatgaa agaaaatggg cgaatctggt taacaaaagc
     5641 ggtgcaatat gcaagttttt atcatgcgtc acggcgacgc agccctcgat gccgccagtg
     5701 attcggttcg tcctctgacc actaatggtt gtgacgaatc tcgcctgatg gcgaactggc
     5761 tgaaaggtca aaaagtggaa atcgaacgcg ttctggtgag cccgttcctg cgagccgagc
     5821 aaacactgga agaagtaggg gattgtctga acctgccttc cagtgcagaa gttctgccgg
     5881 agttaacgcc ctgcggcgat gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg
     5941 aaggtgttgc ttcggtactg gtgatctctc acttaccgtt agtcgggtat ctggttgccg
     6001 agttatgtcc gggcgaaacg ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc
     6061 ttgatgaaag cggaaacggt acattcaact ggcagatgag cccgtgtaat ctgaagatgg
     6121 caaaagctat ctgatgtgtt ccaaattatg cccaatgagg tagattcatt gggcataatt
     6181 gctcatcagg cagcggatat ctgtcaacct cactactatc gaagactctc cttatctata
     6241 aataaaacac tttctacatt cattcggaaa ttattcttct gcacgtatct tgattttgcc
     6301 caaagttaca tgttgaaatg aataatgtct gtttgacact cattgtcatt tattaataca
     6361 gataatagag ttgaataatc aggcaggatg taattgaaac ttgattaaat tctggctata
     6421 gtgtttatat attatattgt caaaagtgga taaatgccaa tcgcattata tattcattgt
     6481 ttgtttttta agctgttgat attcattatt gatatacttt ctattttttc taaatactta
     6541 ttccgtattt tttaaggatg tccttaagat aatctttctt ctgtcaatat attaattaaa
     6601 tctacattta ctctttagta aaattaaatt gcatcagggg tgtggaaaca tcactatgcg
     6661 gccccacacc aacgaataca attttctacg acctgttctt tttggatcgc atgccgtatt
     6721 caatggctgg gtataaacat tggctattga ctgaatcaat gtattgcaat gtttatattt
     6781 ttaattattt atagtttaag gacaaataat gagtaagttt gttaaaacag ctattgctgc
     6841 ggcaatggtg atgggcgtgt tcacctctac ggcaacaatc gctgcgggta acaacggtac
     6901 tgcacgtttt tacggcacca ttgaagactc agtgtgttct atcgttccgg acgatcacaa
     6961 actggaagtt gatatgggtg atatcggtgc cgaaaaactg aaaaataacg gcaccaccac
     7021 gccgaaaagt ttccagattc gtctgcaaga ttgcgtattt gatacttagg aaacaatgac
     7081 cactaccttt actggtaccg tttcttctgc aaatagcggc aattattaca ccattttcaa
     7141 taccgatacc ggtgcggcat ttaacaatgt tagcctggcg atcggtgact ctctgggtac
     7201 ctcttacaaa agcggcatgg gtattgacca gaaaatagtg aaagacactt ctactaataa
     7261 aggcaaagcg aagcagacac tgaactttaa agcctggctg gtcggcgcag ctgatgcgcc
     7321 agatctgggt aatttcgaag caaataccac cttccagatt acttacctgt aaggcatacc
     7381 cccatagcga cactgctatg ggggatttaa atggattttg accgaacaag aataaaaacg
     7441 gatagaaacg tgtatgcctg accattctct ttttcgatta cggattcttc cgtggtgcat
     7501 tgcgctggca atgtcaggga gttatagcag tgtctgggct gaagacgaca ttcagtttga
     7561 ttcccgtttt ctggaattaa aaggcgacac aaaaattgat ctgaagcgtt tttccagcca
     7621 gggatatgtt gagcccggaa aatacaattt acaggttcaa ctaaataaac agccattggc
     7681 ggaagagtac gatatttact ggtatgctgg tgaagatgac gcgagcaaaa gctatgcttg
     7741 tctgacaccg gaactggtag cgcagtttgg tttaaaagaa gacgtggcga aaaatctgca
     7801 atggagccac gatgctaaat gcctgaaatc cggtcaactg gaaggcatgg aaattaaggc
     7861 tgatttaagc cagtccgcat tagtcatttc actgccacag gcttacctcg aatatactta
     7921 tcccgactgg gatccgcctt cacgttggga tgacggcatc tccgggatcg tcgcggacta
     7981 cagcatcaac gcacaaaccc ggcacgaaga aaatggcggt gatgatagta acgagatcag
     8041 cggcaacggg acggtcgggg ttaacctggg gccgtggcgt atgcgtgctg actggcagac
     8101 taactatcaa catactcgca gtaatgatga cgatgaattc agcggcgatg aaactcaaaa
     8161 aaaatgggag tggagtcgct actatgcctg gcgggcgtta ccatcattaa aagccaaact
     8221 ggcgctgggc gaggattacc tcagatccga tatttttgat ggttttaact atgttggtgg
     8281 tagtgtcagt actgacgatc aaatgttgcc tcccaatctg cgcggctacg cgccagacat
     8341 ttccggcgtg gcacacacca cagcaaaagt gaccgtcagc cagatggggc gtgtgattta
     8401 cgaaacgcag gttccggctg gaccgtttcg tattcaggat cttggtgatt ctgtctccgg
     8461 tacgttgcat attcgcattg aagaacagaa cggccaggtg caggaatatg acatcagcac
     8521 cgcctcgatg ccatacctca ctcgtccagg tcagattcgc tataagatca tgatgggccg
     8581 tccgcaagag tggggacacc atgtcgaggg tgaatttttt tctggtgctg aagcttcctg
     8641 ggggatcgct aacggctggt cgttatatgg cggcgcactg ggagatgaaa actatcagtc
     8701 tgcggcgctt ggcgtcggtc gcgatttgtc tacattcggc gcggtcgcgt ttgatgttac
     8761 tcactcgcac accaaactgg ataaagacac cgcttatggc aaaggttcgc tggacggtaa
     8821 ctccttccgt gtgagttatt ccaaagactt tgaccagctc aacagccgcg ttacttttgc
     8881 tggatatcgc ttctcggaag agaactttat gaccatgagc gagtatctgg atgccagtga
     8941 cagcggaatg gtacgcacgg gcaacgacaa agagatgtac accgccactt ataaccagaa
     9001 cttccgcgat gcgggtgttt cggtttatct caactatacc cgccatacct actgggatcg
     9061 cgaggagcag ataaactaca acatcatgct ctcgcactat ttcaatatgg gcagtattcg
     9121 taatgtcagc atctcgatga ctggctaccg ttacgagtat gacaaccagg ccgacaaagg
     9181 catgtacatt tcgctcagta tgccgtgggg cgacaacagt accgttagct ataacggtaa
     9241 ctatggcagt gggacggaca gcagtcaggt cggttatttc agccgtgtcg atgacgcgac
     9301 tcactatcag ttgaacgtcg gcaccagtga caaacacacc agcgttgacg gctattacag
     9361 ccatgatggt tcgctggcgc aggttgacct cagtgcgaac taccatgaag ggcaatacac
     9421 ctctgcgggc ttgtcgttac agggcggcgc aacgcttact gcccacggtg gcgcacttca
     9481 ccgtacccag aatatgggcg ggacacgctt gttgattgat gccgatggcg ttgccgatgt
     9541 tccggtggaa ggtaacgggg ctgctgttta taccaatatg tttggtaaag ccgtcgtttc
     9601 tgacgtcaat aactattacc gcaatcaggc gtatatcgac ctcaacagat tgcctgaaaa
     9661 cgctgaagca acccagtcgg tggtgcaagc cacgctaact gaaggtgcca ttggctaccg
     9721 taaatttgct gtcattagcg gacaaaaagc gatggcggtg ctgcgcctga gcgacggcag
     9781 ccatcctccg tttggcgcag aagtaaaaaa tgataacgag cagacagtgg gccttgtcga
     9841 tgatgacggc aatgtttatc tggctggggt gaaacctggc gaacacatga gtgtgttctg
     9901 gagtggtgtt gcgcattgcg atatcaacct gccggacccg ctgcctgccg atctgtttaa
     9961 cggcttgtta ctgccatgcc agcataaagg caatgtagca cctatcactt cgccggcggt
    10021 caaaccggcg attcaggaac agacacagcg ggtgacgcca acggaacctc cgacttcaat
    10081 ttcagtaaac cagtaacgtg attaaggaat gatccatgtt taatctgact aacactgcaa
    10141 aaatcgttgt cccggcactg gcgctgctgg cgacagcggt cagtttctcc agccacgctt
    10201 cggtgacgcc cgacaggaca cggctggtgt ttaacgaaag cgacaagtcc atcagcgtca
    10261 cgctgcgcaa caacgatccc acactgcctt atctggcgca gagctggata gaggatgcaa
    10321 agggcaacaa aatcagctct ccgcttgcgg tgcttccgcc agtgcagcgc atcgatgcga
    10381 tgatgaacgg ccaggtgaag gtgcaggggc tgccggatat caacaaactg ccggccgacc
    10441 gcgagagcgt gttctatttc aacgtgcgcg aaattccgcc gaaatcaaat aaaccgaaca
    10501 cactgcagat tgcgctgcag acgcgtatca aactgttctg gcgaccgaag gcgctggaga
    10561 acaccagcat gaaaagtccg tggcagcata aggtgacgct catccgtaac ggccaggact
    10621 tcaccgtcaa taacccgacg ccgtactacg tgatcatcag caacgccagc gctaaaaaag
    10681 gcggcaaccc gccggcggga ttcacaccgc tgatgatgga gccgaaaacc tccgcaccgc
    10741 tgaatgcgaa gatggacagc gtgccggtgc tgacttacat caacgactac ggcgcgcgga
    10801 tgccgctgta cttcaaatgc accggcaaca gctgccaggt ggatgaagag cagagccgca
    10861 aaggctgatg ccgggaggga cttatgagtc acaagtttgt agcgggcgca gggttgctgg
    10921 tgctggccgg atggggcatc agcggtttgg ctgttgctgg cagtgatatc aaacaggtgg
    10981 atctgacgct gcgaatccta gtcgatggac cgccgccttg ctccgtgaag ggaagcgctg
    11041 tgaaatttgg caatgtgata atcaacagga tcgatggtac caattaccaa caggatgcga
    11101 aatacaccct caactgcggt aataaagtca gcgatgactt acggatgcag ctcaaaggca
    11161 ataccgccgt gatcaacgga gaaacggttc tcagcacgga tatcgccggc ctgggggtgc
    11221 ggatagaaaa cgccgccgac aactccctgt ttgcagttgg tgaaaataac tggacgcctt
    11281 ttaatgtcaa cactcagcct cagctgaaag cggtgccggt aaaacagagc ggggtgcaac
    11341 tgactccgtc ggagtttaac gccacgatga ccatggtggt ggattaccaa tgaaaaaatt
    11401 cctgctcctt ttactgtgcg ctggttgcgg ggctgtgctg gcggcggaag atgacatcac
    11461 attccacggt acgctgatgt cgccgcccgc ctgtagcatc agcggcgggc aaaccatcga
    11521 agtgcaattt cgcgatctca ttatcgacag cattgatggc aattatggtc gtcagaaagt
    11581 ggactacgaa ctgagctgcg actcggatgt ccgcgatccc gagtggaata tgacactctc
    11641 ctggacgggg actgccacct cctacaacga ctctgctatt gagacggatg taccgggctt
    11701 cggaattgag cttcagcatg acggtcagcg ttttaagctc aacgagccgc tttccattaa
    11761 cgccaccgat tttagccaga agccaaagct ggaggcggta ccggtcaaag cggctgatgc
    11821 ggtgctgacg gacacgacct tttctgctta cgcaacctta cgggtggatt accaatgaaa
    11881 acagcacaca tttattcacc cggttatgcg ctgttagcag gcctgctgct gaccgccagc
    11941 acaggagcac aggcagtcga caacaacctg catttttacg gcaacctgct gagtaaatcc
    12001 tgcacgctgg tggtggatgg tgccaacctg gcagaaatcc attttcccac cgtcagcaac
    12061 cgggatttga tggtatctgg gcagtccgcg catctgccgg ttgtgtttaa gctgaaagac
    12121 tgcaaagggc cggcgggata caacgtcaaa gtcacgctga gcggcaccga agacagcggt
    12181 cagccgggtt ttctggcagt cgatgacacc tccaccgcgc agggcgtggg gatagggatg
    12241 gaaacgctcg acggcaagcg ggtggatatt aacaacacca ccggggcgac ctttgcgctt
    12301 accaacggca acaatgatat taatttccgg gcctggatct aggcgaaaag cggtcgtgat
    12361 gtaaccatcg gtgatttcac cgccagcctg acagcaactt ttgagtat
//
LOCUS       DAJTOR010000122        12401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTOR010000122 DAJTOR010000000
VERSION     DAJTOR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.122"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1317
                     /locus_tag="OO249_002972"
     CDS             <1..1317
                     /locus_tag="OO249_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR5637194.1"
                     /translation="AQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTREN
                     FVQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLIQETERRR
                     RERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFE
                     AERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTK
                     SIAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAET
                     KAVELTVRAKAEKEAAEMQAAAIVKLAEATRKKGLAEAEAQRALNDAINVLSDEQTSL
                     KFKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQA
                     LSAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1556..1615
                     /locus_tag="OO249_002973"
     CDS             1556..1615
                     /locus_tag="OO249_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5637195.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(1735..3168)
                     /gene="hldE"
                     /locus_tag="OO249_002974"
     CDS             complement(1735..3168)
                     /gene="hldE"
                     /locus_tag="OO249_002974"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5637196.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3216..6056)
                     /gene="glnE"
                     /locus_tag="OO249_002975"
     CDS             complement(3216..6056)
                     /gene="glnE"
                     /locus_tag="OO249_002975"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5637197.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMMLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6079..7380)
                     /gene="ygiF"
                     /locus_tag="OO249_002976"
     CDS             complement(6079..7380)
                     /gene="ygiF"
                     /locus_tag="OO249_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5637198.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7622..8242
                     /locus_tag="OO249_002977"
     CDS             7622..8242
                     /locus_tag="OO249_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5637199.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKERWMN"
     gene            8306..9544
                     /gene="cca"
                     /locus_tag="OO249_002978"
     CDS             8306..9544
                     /gene="cca"
                     /locus_tag="OO249_002978"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5637200.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9701..10522)
                     /gene="bacA"
                     /locus_tag="OO249_002979"
     CDS             complement(9701..10522)
                     /gene="bacA"
                     /locus_tag="OO249_002979"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5637201.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10613..10981)
                     /gene="folB"
                     /locus_tag="OO249_002980"
     CDS             complement(10613..10981)
                     /gene="folB"
                     /locus_tag="OO249_002980"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5637202.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11086..11703
                     /gene="plsY"
                     /locus_tag="OO249_002981"
     CDS             11086..11703
                     /gene="plsY"
                     /locus_tag="OO249_002981"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5637203.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11716..>12401)
                     /gene="ttdR"
                     /locus_tag="OO249_002982"
                     /gene_synonym="dan"
     CDS             complement(11716..>12401)
                     /gene="ttdR"
                     /locus_tag="OO249_002982"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCR5637204.1"
                     /translation="VDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQV
                     HFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQSL
                     QELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGIML
                     RSEWDVLPFLESGKLVQVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLVAWCQQRLG
                     KPDEGYQVM"
BASE COUNT         2991 a         3535 c         3096 g         2779 t
ORIGIN      
        1 gcgcagacgt tggggcaacg cacgctatca ccagaagact tacgtatgtt ggttgaagat
       61 aaatttgtcg atgccctccg tgcaacagct gcacaaatga ccatgcatga gttacaggat
      121 acccgcgaga acttcgtgca gggagtacaa aatactgtcg cagaagacct gtcgaaaaac
      181 ggtcttgagc tggaaagcgt ttcacttacc aactttaacc agacctcgaa agaacatttc
      241 aatccgaaca atgcctttga cgccgaaggt ttaaccaaac tgattcagga gacggagcgc
      301 cgtcgtcgcg aacgtaacga agttgaacag gatgtagaag ttgcggtgcg tgagaaaaac
      361 cgtgatgcac tttcgcgcaa gttggagatt gaacaacaag aagcgtttat gacgcttgag
      421 caggagcagc aggttaaaac ccgtactgcc gaacagaatg cacgtattgc ggcttttgaa
      481 gctgaacgtc gtcgtgaagc agagcagacg cgaattctgg ctgaacgaca aattcaggaa
      541 acggagatcg agcgcgagca ggccgtccgc tcaagaaagg ttgaggctga acgtgaagtt
      601 cgtattaaag agatcgaaca gcagcaggtc accgaaatcg ccaaccagac gaaatcgatc
      661 gctattgccg ccaaatcgga acaacagtcc caggcagaag cgcgtgctaa ccttgcactc
      721 gcagaagcag taagtgcgca acaaaacgta gaaaccactc gccagaccgc agaagccgat
      781 cgtgctaaac aagttgccct aatcgctgcc gcgcaggatg cagaaaccaa agcggttgaa
      841 ctgaccgtgc gggcgaaagc agaaaaagaa gccgcagaga tgcaggcggc ggctatcgtt
      901 aagttagccg aagctacacg taaaaagggt ctggcggaag cagaagcaca acgtgcgctg
      961 aacgatgcta tcaacgtact ttctgatgaa caaaccagcc ttaaattcaa actggccttg
     1021 ttgcaggcgc tacctgcggt aatagaaaaa tccgttgagc cgatgaaatc tattgacggt
     1081 atcaagatta ttcaggtcga tggtctgaat cgtggcagcg ctgcgggtga tgcaaacacg
     1141 ggtaatgtgg ggggcggaaa cctggcggaa caagcattat cagccgctct ctcttaccgc
     1201 acacaggcac cgctgattga ctccttgctc aatgaaattg gcgtttcagg cggctcactg
     1261 gcggcattga cttcaccctt aacctcaaca actcccgtcg ccgaaaacgt agaataaatt
     1321 ttcagctatg aacgtccctg gcacaaaaag ccggggattt tttatatctg cgttccgcta
     1381 aaaactgcaa atgatcaggt cgttgcaacg ttttgcgtga ccgctcgggg aaggcaaaac
     1441 tgcctctggg aaagcgttgc tcgaggtccg gcgctcatca acaatcgggg agtcagcaaa
     1501 gggctgaaac gggaaagccc ctcccgagga aggggcctta cagaaggaaa gggttatgat
     1561 gaagctcgtc atcatactga ttgtgttgtt actcgtaagt ttcgcagctt attaacagcc
     1621 aatcagaggg gggagaaatc ctccctcacc cttgttcctt tactttaggt tgaaaaaaca
     1681 acagcgtcaa taggcctgcc tgctacgaag cgagatctgt gaaccgcttt ccggttagcc
     1741 ttttttatcc tgttggatct tcttgatgat gttagtggtc gagcaaccgt cttcgaagtt
     1801 gagcaccagc acttcgccgc cgttggccca gacttcttta ctcccggcga tctcttctgg
     1861 tttatagtcg ccgcccttca ccagtaaatc tggcaggatc ccggcgatca agcgctgcgg
     1921 cgtgtcttct tcaaacgaca ccacccagtc gaccgcttcc agtgcgccca gcacaatcat
     1981 acgctgttcg agcgggttaa cggggcggga atcccctttc agccgtttgg tggaggcatc
     2041 gctgttcaca gcgacaatca gacggtcacc cagtttgcga gcatttgcca gataggagac
     2101 gtgcccggca tgcaggatgt caaagacacc gttggtcatt accacctttt cgccgcgttt
     2161 acgcgctgcc gctacggcca gcttcagttc ctcttcggtc atcacgccaa agccggtatc
     2221 tgcgcgccca cgcactgcat tttccagctc gatcggcgaa accgtggagg ttcccagttt
     2281 gccgaccacc acgccagccg ccgcattggc aaagaagcag gcttcttcca gcgaattacc
     2341 cgctgccagc gttgccgcca ggacgccaat caccgtgtcg cccgcaccgg taacgtcata
     2401 cacttcctgc gcctgggttg gcatatgcag cggcgcttta cccggttgca gcagcgacat
     2461 accctgttcg gaacgggtca ctaacagagc cgagagttcg taatcggcaa tcagtttcat
     2521 gccgcgctca acaatctctt cttcggtctt acatttaccg acaacagctt caaattccga
     2581 gagattcggc gttaacagcg tagcgccgcg gtagcgctca aaatcggtac cttttggatc
     2641 aatcagcacc ggaacgcccg ctttacgcgc cagttggatc atctgctgta cgcttgccag
     2701 cgcacctttg gcgtagtcag aaagcaccag cgcgccaatc gaactcagcg cctgattaat
     2761 ccgctcgtgc agcggctgcg gatcaacgcc ttcgaaacct tcttcgaaat ccagacggat
     2821 cagctgttgg ttgcgggaaa gtacccgtaa tttggtgatg gtcggatgcg tcggtacaga
     2881 aacgaagtcg catttgacgt tgacgtcggc cagagattta ctcagcgcgc gcgctgcatc
     2941 gtcaatgccc gtcaacccga ccaggcgtgc attagcaccg agagaagcga tattcatcgc
     3001 cacgttagcc gcgccgcccg gacgttcttc gatggtattc actttaacca cgggcaccgg
     3061 cgcttccggc gagatacggc tggtgggacc gtaccagtaa cgatccagca ttacatcacc
     3121 aaccaccatc actcctgcac gttcaaactc tgacagcgtt actttcattc ctgtctcctg
     3181 agagattcaa aatttgcgcg cgataatacc atacttcatt cttccaccag ccacttctgc
     3241 cagcttgccc gcaccagatc acgctctgcg gtgaagcaat cctccgacac atggcccggc
     3301 aattcctgta atgccagatg atgaagttca tcgcgcagcg tcgtgtaagc acgggtcagc
     3361 gccatcgctt cctgctcttc cataatgtcg ttttgcgcca gcagctccag aatacgcacg
     3421 ttgtctgacc agcgcgttaa cttcggtttt tcatgagcgt agcgcaacac cagatattgg
     3481 gtaataaatt cgatatcggt aattccgcct tcatcggctt tgatatcaaa gcgattacga
     3541 tgtttattgc cgagatgagc gcgcattttc tcgcgcattt cccgcacttc cgtttgcaga
     3601 gttttacctt cacgcggcag catcataatc tcgcgacgca ctgcgtcaaa gtgcgcggtg
     3661 agctgcggat cgccgtacac tacacgcgca cgcaccagcg cctgatgctc ccacgtccag
     3721 gcctcgtttt tctgataatc agcaaatgct tctgcggatg tcaccagcat tcccgccgcc
     3781 ccggacggac gcagtcgtgc atccacttca tacaaaatgc cggaagaggt tcgcgtattg
     3841 aacagatgca taatgcgttg cgccagacgc agataaaact gtcgcccgtc gatttcccgc
     3901 tcgccgtcag tcatcgcatc cattgggcaa tcatggagga agatcaggtc aagatcggaa
     3961 ctgtagccta actcccagcc gcccagcttg ccgtagccga ccaccgcaaa accacggcct
     4021 tcgcgatcgt tgaggtgatt tggcttaccg tagcgggcca ccatttgaac ccacgcctgc
     4081 tgaacgacgg catctatcat agcctctgcc agccaggtta agtgatcgct cactttcatc
     4141 accggtagcg taccagcgat atccgctgcg gcgatgcgta acagctgcgc ctgtttgaac
     4201 tgacgcagcg cctcaagctg ttgctcttcg tcatcttccg gcacgcgcag caaatactgg
     4261 cgcaactcat cgcggtaggc atcggtcgcc gtcggctggt aaagagtgtt tggatcgagc
     4321 aattcatcca gcaataatgg ataacgcgcc agctggctgg caatcatcgg cgatgcggca
     4381 cacagagaaa tcaaatgttt aagcgccgca gggaattcac tgagcaattc taaataggtg
     4441 gtgcgggtaa caatccccac cagcaaggcg gtaatgcgcg acagcgtaac ggcagcgtct
     4501 tcacgcgcac agacatcact tagcagatgt ggcatcagat ggtcgagcac ctgacgtcct
     4561 cgcggcccga tagtacgctt atccagctct ttgcggaaat cagcaatcag cgttaggacc
     4621 tgtttgcgat catcctcgct aagatgcgcc agcactggcg tagtgtcatc ttcctgcaac
     4681 gcatcctgcc acagctcacg ccactgttcc gacagcgact cttcctgagt ttcactttcg
     4741 tcgtcgccaa tcaattcatt aaacacccgg cgcacattgg tcatatgtgc ggtcagcgcc
     4801 ccggtcagtt gcggccagtc agcaaagtcc atcgcccacg ccagccgcgc acggttaagc
     4861 tcatcagaag gaagcgtttg ggtttgttcg tcgttaatgc tttgcagcag gttttccaga
     4921 cgccgcagga acagatatgc cactcgcaat tgttcagcat cgttttcaga aagcagatgc
     4981 agcgcggcaa tgacgctgag cgttggtagt aaagagcgcg actgcagcga cggttcgcgc
     5041 ccaccgcgaa tgagctggaa cacctgaacg ataaattcaa tttcgcgaat gccgcctgcg
     5101 ccgagtttga tattgtcggt caaaccacgt cgacgcactt cacgggcaat catccctttc
     5161 atgttgcgca gcgactgaat cacgctgaaa tcgatgtaac gacggaaaac aaacgggcgc
     5221 agcatcgcac gcaactcgtt agcatagacg ccttcgctat cgcccataat ccgcgccttg
     5281 accatcgcgt aacgctccca gtcgcgcccc tgctcctggt aataatcctc caacgcggca
     5341 aagctcagca ccagcgggcc actttcgcca aacggacgca gccgcatatc cacgcgatag
     5401 acgaagccat cctgcgttgg ttgatccaac actttaatca gccgctgccc catgcgggta
     5461 aaaaactgcg cgttatccag ttcccggcgt ccgccctgcg tacaaccatg ttccggccag
     5521 gcaaaaatca gatcgatatc tgaggagaaa ttcagttccc caccgcccag cttacccatg
     5581 cctaaaatca gcagcggttg cgcttcgccc tgcgcattgc acggcgttcc ccactcgcgg
     5641 cagcaagcgt catacagcca gtcacgcgcg gcgacaatca gcgtctccgc cagatggctg
     5701 agctgctgca atatactctc ttcagtaacc aaagccagcg tttgcgccca ggcgatgcgc
     5761 accataatgc gccgccggaa tagccgcagc tcgcgcatta acccggcttc gtcactcaca
     5821 ttgctgagcg cctcctgcaa ccatgccgcg taatgctgcc attcatcagc ctgcggcgat
     5881 tggctttcca gttccgtcag ccactctgga tgcgcaatca cgctgtcctg cacaaaatca
     5941 ctgaaagtaa gtactgactt cgcctgtgcg ctaagtgatt tctcggctaa aggctctggc
     6001 agccgctcaa caacggtctg ccagtactgc tgtaacggtg aagagagcgg cttcataagc
     6061 gattttatcc ttgcctgatt aacgttttcc gctgtgcaac cagaacggtt cctgattgtt
     6121 tgcctcatta cggaaatgtt caatttcaat gcgttgcccg gtcgcaatag cgtgacgcag
     6181 cccctgccag ttctccagcc aggcttgcgc gacgacagga tcatagtaac ccgccagcaa
     6241 cagtattgag tcaatatcac gcgtcagacg tggcaactgg tcacggtagc gatcgcccaa
     6301 cggctggcag aaaacgcttt tcagttcagc ggcatggcgg gaaagatgga tatcggcaaa
     6361 gcgtttgaag gaatcgctca ttttactctg cgctttggca tctaaaaacg gctgccaggc
     6421 tttgcttacc agccattcgg tcaacgccag cttcgccatt gccgttttgg tagagtagac
     6481 cgccgtcacg gcagaaaccg cagaagcgat ggtcgcttcg cattgagtca gcaaatcgcg
     6541 taagtgagtg ctcgctttgc gcggcacaat accaccgaac agcatcagtg tatgacggac
     6601 cagaccaatg gctgccagca cctgttcttt cgccgcatcg ttgccgcgta cccacagttc
     6661 ttcatgatac tgccattgcg ctaacgccag ctcgagcgcc gcttccagcc cctgttccac
     6721 atcggctttt gccgcaacat gcaaaatggt ggtcggtttg atttcacgcg ccggattgcc
     6781 ctgcgccaga tgatagccac gcgccgcttt gctcaggctg ccctggcgta atccggtttg
     6841 cgataccagt tggttcgcca gtttcagcac cgcgcgcgtg tcgccgctaa gcagttccag
     6901 ttccagctca cagataggtt cagcaaattc ccccgctttc acctccccct gatcgagagc
     6961 gatttcaatt cggctgtcat cgaccgccac cagccatttt tcgcgataaa aatcggtgct
     7021 gaacagcggt tgtacgcggg aagcgagatc ggcgggcaat tcgccgttcg gccagacttc
     7081 cgtcggtaac tgcgccaggt cgagcgtcgg ttcgctcaac gccacgttat attccgggcg
     7141 ctggtgtaag ccgcctgtca ctcttcctgc aactttcatg gtcatctcat agcgaccgtt
     7201 ttcgccacga atacgtaagc ccatatcgtg cccacgcagc cagttatccg gcgtttcgta
     7261 gtaaatattc agcaactgca cggggtcatg gtgctcgccg cccagcgtat tgagatggtc
     7321 acgcaacgcc tcaacggcac tgtgattaac aataaacttt aattcgattt cctgagccat
     7381 ggcctttact tatcggttat gtcacataag tgacgatgaa cggcgaactt aatgcgatct
     7441 tttttgtcag tagatagtat tttgcgccaa attgccatgc aacgggcaat ttgacgggcg
     7501 taaaagtttg aagcagtggc aagacctgac acagaggatt ccgattgatg tcgaatgctt
     7561 tgcgtagcga cactgatgcc actactatcg ttccactttc catgacaata acgacagcct
     7621 gatgccaaaa ttacgcctga tcggattaac tttactcgca cttagcgcca ctgccgtctc
     7681 acacgctgaa gaaacgcgct atgtttctga tgaactgaat acctgggtcc gtagcggtcc
     7741 gggagatcat tatcgcctcg tgggcacggt taacgccggc gaggaagtga ccttattaca
     7801 aactgacgcc aacaccaatt atgcccaggt gaaagacagc tctggccgta ccgcctggat
     7861 cccgttgaaa caacttagca ctgagccaag cctgcgctcc cgtgtgccag atctggaaaa
     7921 tcaggtcaaa accctgaccg ataaactcac caatatcgat aacacctgga atcagcgcac
     7981 cgcagaaatg cagcaaaaag tggcgcagag cgacagcgtg atcaacggat taaaagaaga
     8041 aaatcagaaa ttgaaaaacg agctgattgt cgcgcagaaa aaggtcgatg ccgccagcgt
     8101 acagctggat gacaaacagc gcaccatcat catgcaatgg tttatgtatg gcggtggcgt
     8161 gctggggctt ggcttgctgc tcggtctggt actgccacac ctgatcccaa cgcgcaaacg
     8221 caaagagcgc tggatgaact aaatcgcctt cttcgttgca ctgtcttatt attaggaatt
     8281 gtgtatttga cgaagggatg atggcgtgaa gatttatctg gtcggtggtg ctgttcggga
     8341 tgcattgtta gggctaccgg tcaaagacag agattgggtg gtggtcggca gtacgccaca
     8401 ggagatgctc gacgcgggct accagcaggt aggccgcgat tttcctgtgt ttctgcatcc
     8461 gcaaacgcat gaagagtatg cgctggcacg taccgaacgg aaatccggtt ccggttacac
     8521 cggttttact tgctatgccg caccggatgt cacgctggaa gatgatctta agcgtcgcga
     8581 tctgaccatt aatgcgctgg cccaggacga taacggcgag attatcgacc cgtacaacgg
     8641 tctgggcgat ctgcaaaatc gtctgttgcg ccatgtttcc cccgcttttg gcgaagatcc
     8701 gttacgcgtt ctgcgcgtgg cgcgttttgc tgcgcgttat gcccacctcg gttttcgtat
     8761 tgccgatgaa actctgacgc tgatgcgcga gatgacccat gcgggtgaac tggaacacct
     8821 gacgcctgaa cgggtatgga aagagacgga aagcgccctc accacccgca atccccaggt
     8881 gttcttccag gtactgcgcg attgcggtgc acttcgcgtt ttattcccgg aaattgacgc
     8941 cctgtttggc gttccggccc ctgccaggtg gcatccggaa atcgacaccg gtattcatac
     9001 cttaatgacg ctctcaatgg cggcgatgct gagtccgcag gttgatgtcc gtttcgcaac
     9061 tttatgccac gatctcggta aaggactgac gccgccagaa ctttggccgc gtcatcatgg
     9121 gcatggcccg gcgggtgtta agttagtgga acaactatgc cagcgtctgc gcgtgccaaa
     9181 tgaaattcgc gatttagcca gactggtggc tgagtttcac gatctcatcc acaccttccc
     9241 aatgctgaac ccaaaaacca tcgtcaaatt atttgattcc atcgatgcct ggcgtaaacc
     9301 gcagcgtgtc gagcaactgg cgctgaccag cgaggctgac gtacgcggca gaaccggttt
     9361 tgaatcagcg gactacccgc aaggccgttg gttgcgcgaa gcctgggaag tggcgcagtc
     9421 agtgccgaca aaagcggtcg ttgaagcggg atttaaaggt gtggagattc gcgaggagct
     9481 gacccgacgg cggattgcgg cggtagccag ttggaaggaa caacgttgcc caaagcctga
     9541 ctgagtataa aactaaacgt taacacgaac catcccctcg ccccttcggg gagagggtga
     9601 ggggaacaga ccagcaccgg tgcgaacaat cacccccacc ccggccctct ccctacagag
     9661 cgagggagaa aaccaggcct gctcccgaac cttttctcaa tcaaaagaac acgacgtaca
     9721 ccgcagccgc cacaataaag cgataaatgg cgaacgggat aaacgaaatg cgcttaatca
     9781 attgcaggaa ggttttaatc gctatcagcg ccaccacaaa agcggtgata aacccaacgg
     9841 caaacatcgg gatatcgccg gttgtcagga agccccagct tttgtagaga tcgagcgccg
     9901 ttgcgcccat catcatcggt accgccagca ggaacgagaa ctcggaagca gcgtaacggc
     9961 tcacgcccat cagcatccca cctgaaatgg tggccccgga acgggagaaa cccggccaca
    10021 gcgccagaca ctggaaacag ccaatcataa acgcctgacg ataggtcata tcatcaagac
    10081 ccggcgcacg cggctctttc ggcttcaggc attcggcggc aatcagcaac aaaccgccaa
    10141 cgaccagtgc atacatcaca tttatcgggt taaacaatga cttaatcgtg tcgtggaaca
    10201 acagccccag taccaccgcc ggaatcatcc ccagcaaaat gtggatcagc gttaaacgac
    10261 ctttgctttc tccttcgtgc tgcaacgggc ggccaaagtg gatgccaatc aggccaaaca
    10321 gacgccgcca gaacatcact actaccgcca gaattgatcc taactggatc acaacttcaa
    10381 aggttttcgc cgtgtcgccc tcgaacccca acaagtgacc gacaataatc atatggcccg
    10441 tgctggatac cggcagaaat tctgtcaatc cttcgaccac acccaatatt gccgctatca
    10501 gcagcgagtg catatcgctc atcaataaac ccctaaatta ttaaaatgta ccgcttgtcc
    10561 ggactactgc agtaagacca ggttataacc gtttggttta acagctgtaa aattaattat
    10621 tttctttcag attattgcca cgctcaatga ttacaccaac attcgccgcc cgcgccactg
    10681 cgcctggctt gctgagtttg atgcgcaccc acggcgagtt gaagcgtgct aacagcagct
    10741 ccgccacctc ttcagccacg cgttccacca gcgcaaaacg cgccccctcg acgtggctga
    10801 ccaccgtttc tgcaatgtca gcgtaactga ggcaatccgc cacatcatca cttttcgccg
    10861 ctttacggtt atcccatgcc atttcgatat cgaacactaa cttctgttcg atggtctgtt
    10921 cccagtcgta aacaccaata gtggtgatta ccgaaagttg ctctataaat acaatatcca
    10981 tcacgtcctg cctgcttttt ggctaaccgg ataccacttc cggcgaaatg tgcgtattat
    11041 ccacagattc atcgttgaac acgaattttc aaaacggaac agcttatgag tgcaatcgcg
    11101 cctggaatga tcctcatcgc gtacctctgc ggctccattt ccagtgccat tctggtttgc
    11161 cgcttgtgtg ggctgcccga tccgcgaacc agcggctccg gcaatccagg cgcaaccaat
    11221 gtgttacgta tcggtggcaa gggagcagcc gtagcagtac tgattttcga cgttctgaaa
    11281 ggaatgttgc ccgtctgggg cgcgtatgaa ttaggtgtca gccccttctg gctaggctta
    11341 attgccatcg ccgcctgtct tggacacatc tggcccgttt tcttcggatt taaaggagga
    11401 aaaggcgttg ctaccgcttt tggtgccatc gcacccattg gctgggatct caccggagta
    11461 atggcgggaa cctggttact gaccgtgcta ttgagcggat actcgtcgct gggagcgatt
    11521 gtcagtgcac tgattgctcc gttttatgtc tggtggttta agccacaatt caccttccca
    11581 gtttcgatgc tctcttgcct gatcctgctg cgtcatcatg acaacatcca gcgtctgtgg
    11641 cgtcgtcagg agacaaaaat ctggacgaaa ttcaaaagaa agcgcgaaaa ggatcccgag
    11701 tgatttctgg tggatctaca tgacctgata gccttcatcg ggcttgccca gccgttgctg
    11761 gcaccatgcc accagaaatt ccacgcagac acgtaatttc atgctgcgat agagcggctc
    11821 ccggtaaaca gcccagatat tggcgctttg tgcatactcc ggcaatactt gcaccagttt
    11881 gccactctcc agaaacggca acacatccca ctcggaacgc agcataatcc ctttgccctc
    11941 cagcgcccat tgcaacacta tctcgccgct attggaggaa agatgcccgc taaccttcac
    12001 cgattttttc tcctgaccgt tccccaactc ccatattccg tgggtcatat cgcgctcttt
    12061 ggtcaccaga cagtcatgac gacttaattc ctgtaatgat tgtggctgcg gatatttttg
    12121 cagatactca ggtgctgcgc ataatattct tttatttttc gttaacagat gcgcaatata
    12181 ataatcagga atttcgtcat taatacgaat atccagatca atattatcct gcactaaatc
    12241 aatttgccga tcgaacaatt caaaatgcac ctgtaactca ggataattgc gcataagttc
    12301 ggtaatggct ggcgcaatat ggctgcgccc aaaaccaaaa ctgcaaccaa tacgaatcat
    12361 cccctccggg cgcgttttga tttgcgtgac gtcatcgacc a
//
LOCUS       DAJTOR010000123        12215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTOR010000123 DAJTOR010000000
VERSION     DAJTOR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.123"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..565
                     /locus_tag="OO249_002983"
     CDS             <1..565
                     /locus_tag="OO249_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5637205.1"
                     /translation="DGLVNNAGVNFPRLLVDEKAPAGQYELNEAAFEKMVNINQKGVF
                     LMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSCYAATKAALNSFTRSWSKELGKHG
                     IRVVGIAPGILEKTGLRTPEYEEALVWTRNITVEQLREGYTKNAIPIGRAGRLAEVAD
                     FVCYLLSERASYITGVTTNIAGGKTHG"
     gene            575..982
                     /locus_tag="OO249_002984"
     CDS             575..982
                     /locus_tag="OO249_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5637206.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            982..1476
                     /locus_tag="OO249_002985"
     CDS             982..1476
                     /locus_tag="OO249_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5637207.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            1542..2339
                     /locus_tag="OO249_002986"
     CDS             1542..2339
                     /locus_tag="OO249_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5637208.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2350..3192
                     /locus_tag="OO249_002987"
     CDS             2350..3192
                     /locus_tag="OO249_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000437910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5637209.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITTALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLSRF
                     FAPA"
     gene            3226..4455
                     /gene="sorE"
                     /locus_tag="OO249_002988"
     CDS             3226..4455
                     /gene="sorE"
                     /locus_tag="OO249_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5637210.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            4838..5527
                     /gene="pepE"
                     /locus_tag="OO249_002989"
     CDS             4838..5527
                     /gene="pepE"
                     /locus_tag="OO249_002989"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5637211.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEDNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(5618..7249)
                     /locus_tag="OO249_002990"
     CDS             complement(5618..7249)
                     /locus_tag="OO249_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5637212.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTAALTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(7469..11157)
                     /gene="metH"
                     /locus_tag="OO249_002991"
                     /pseudo
     CDS             complement(7469..11157)
                     /gene="metH"
                     /locus_tag="OO249_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            11357..12181
                     /gene="iclR"
                     /locus_tag="OO249_002992"
     CDS             11357..12181
                     /gene="iclR"
                     /locus_tag="OO249_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5637213.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2809 a         3303 c         3250 g         2853 t
ORIGIN      
        1 cgacggtctg gtcaataacg ccggggtcaa tttcccgcgt ctgctggtcg atgagaaagc
       61 gcctgccggg cagtatgaac tcaacgaagc tgcattcgaa aaaatggtca atatcaacca
      121 gaaaggcgtt tttctgatgt cgcaggcggt ggcgcgacag atggtcaaac aacatgatgg
      181 cgtgattgtg aatgtttcct cagaaagtgg actggaaggc tcagaaggcc aaagctgtta
      241 cgccgctacc aaagccgcgc ttaatagctt cacgcgatcc tggtcgaaag aattgggtaa
      301 acacggtatc cgtgtggtcg gtatcgcgcc ggggattctg gaaaaaacag gactgcgcac
      361 gccggaatat gaagaagcgc tggtgtggac gcgcaatatc accgtcgagc agctgcgtga
      421 aggctatacc aaaaacgcca ttcctattgg gcgcgccgga agattagcag aagtcgctga
      481 ttttgtttgt tatctgctgt ctgaacgcgc cagctatatc accggagtaa ccactaacat
      541 tgcgggcggc aaaacgcacg gctaaggagg ctttatggtc aatgccattt tttgcgccca
      601 cggcaaatta gcttgcgcca tgctggaatc ggttcagatg gtgtacggcg acgccagggt
      661 cgaggcagtt gaatttgtgc ccggcgagaa cgccggagac atcgtagcaa agctggaaaa
      721 gttagtaagc attcacaatc aagatgagtg gctgattgcc gtggatttac agtgcggcag
      781 cccgtggaat gcggcagcga tgcttgcgat gcgcaatcct cgtctgcggg taatcagcgg
      841 cctttctctg ccgctggcgc tcgaactggt tgataaccag gacagcatga atgttgatga
      901 actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc gttgtctgga aacaactggc
      961 aacggcagag gaggatttct gatgaacatt acgctcgccc gcattgatga ccgcctgatc
     1021 cacggtcagg tcactaccgt ctggtcgaaa gtggctaacg cccagcgcat tattatctgc
     1081 aatgacgaag tttataacga tgaagttcgc cggacattat tgcgtcaggc tgcgccaccg
     1141 ggaatgaagg ttaacgtggt caatattgaa aaagccgtcg ctgtttatca caacccgcaa
     1201 tatcaggacg aaaccgtttt ttatcttttc actcgcccac aggatgcctt agcgatggtt
     1261 cgccaagggg taaaaataga caccctaaat attggcggca tggcctggcg gcccggtaaa
     1321 aaacagttaa ccaaagcggt ttcattagat gatgatgata ttaacgcatt tcatgagtta
     1381 aataatcttg gcgtcatact ggatttacgc gtcgtagcgt cagatccctc aatcaatatt
     1441 atcgacaaaa taaacgaaca gttaatcgca aattaaataa tagcgcttgt gtacatatgc
     1501 tttaacaggc agggatgacg acacttaaaa ggtgaaaaat catggaaata agtaccctac
     1561 aaatcattgc tatatttctt ttttcctgta ttgccggaat gggcagcgtg ctggatgaat
     1621 ttcagactca tcgcccgtta attgcctgta cggtgattgg tttaattctc ggtgatttaa
     1681 aaaccggaat aatactcggt ggtacgctgg aattgatagc tctcggctgg atgaacgtcg
     1741 gcgcggcgca atctccggat tctgcactcg ccagcataat ctccgccatt ctggttatcg
     1801 ttggtcagca aagcatcgcc accggaatcg ccatcgcgtt gcctgtggct gcggcaggcc
     1861 aggtgctgac tgtgtttgcc cgtaccatca ccgtagtgtt ccagcacgcg gcggataaag
     1921 cagcggaaga agcacggttt cgcactctcg atattctgca tgtctccgcg tttggcgtgc
     1981 aggcgctgcg cgttgctatt ccggcactga ttgtctcact gttcgtcagc gccgatatgg
     2041 tgagcaatat gctgagcgcg ataccggaat tcgtgacgcg cggtctgcaa attgctggcg
     2101 gttttatcgt ggtggtcggt tacgccatgg tgctacgtat gatgggcgtg aaatatttga
     2161 tgcctttctt tttcctcggt ttcctcgcag gtggctacct cgatctcagt ctgctggcgt
     2221 tcggtggcgt cggcgtgatc atggccctgg tctacatcca gctaaatcca cagtggcgta
     2281 aagctgaacc tcaactccag gccaccgcct ccaccgccct tgaccaactt gacgattaac
     2341 ggagccatca tggaacagag aaaaattaca cgcagcgatc tggtgagcat gtttctgcgc
     2401 tccaacctgc aacaggcgtc ctttaacttt gaacgtattc acgggctggg tttttgctac
     2461 gacatgatcc ccgccatcaa acgactttac ccattaaaag aggatcaggt tgcggcgctc
     2521 aggcgacacc tggtgttctt caataccacg ccagccgtat gtggcccggt catcggcgtc
     2581 actgccgcca tggaagaggc gcgggccaac ggcgcggaaa ttgatgacgg taccattaac
     2641 ggcatcaaag tcggtctgat gggaccattg gcaggagttg gcgatccact ggtctgggga
     2701 acgctgcgcc cgattaccac cgcgctcggc gcatctctgg cactttcggg caacattctc
     2761 ggcccgctgc tgttcttctt tattttcaat gcggtgcgtc tggcgatgaa gtggtatggc
     2821 ctacagctcg gctttcgcaa aggggtgaat atcgtcagcg atatgggcgg gaatgtgctg
     2881 caaaaactca ccgaaggcgc gtcgattctc ggactgtttg tgatgggcgt gctggtcacc
     2941 aaatggacgt caatcaacgt accgttggtg gtttcacaaa cgcatgccgc cgatggctcc
     3001 accgtcacca tgaccgtgca gaacattctc gaccaacttt gccctggttt gctggcgctc
     3061 ggtctgacgc tgcttatggt tcgtctgctc aacaaaaaaa ttaacccggt atggctgatt
     3121 ttcgccctgt ttggcttagg gattatcggt aatgcgctgg gcttcctgtc cagattcttc
     3181 gccccggcat gactgccggg gccatcgctc aacatgaggt ggtttatgaa aacaacagct
     3241 ctgcgtcttt atggtaaacg tgatttacgc ctggaaacct ttgaccttcc tgaaatgcag
     3301 gaggatgaaa tcctcgcgac ggtggtcact gacagcctgt gcctctcttc ctggaaagag
     3361 gccaatctgg gtgaaaacca taaaaaagta cccgacgatg tggcgaccaa ccccatcatc
     3421 atcggccacg agttttgcgg cgatattctg gccgtgggta aaaagtggca gcacaaattc
     3481 cagccgggtc agcgttatgt gattcaggcc aacctgcaac tccccgaccg cccggactgc
     3541 cccggctact ccttcccgtg ggtgggcggc gaagccacgc atgtggttat tcccaacgag
     3601 gtcatggaac aagattgcct gctggcatac gacggcgaaa cctattttga aggctcactg
     3661 gttgaaccgc tttcctgcgt gattggcgcg ttcaacgcca actatcatct tcaggaaggt
     3721 agttataacc acacgatggg gattcgcccg caagggcgca tgctgatcct cggcggcacc
     3781 ggaccaatgg gactgttggc gattgattat gcgctacatg gacccgttaa cccgtcgcag
     3841 ctcgtcatta ccgataccga caacgataaa ttgagttatg cgcgcaagca ctatccatca
     3901 gaaccgcaaa cactgattca ttatctcaat gccgccgatg cagcatttga tacgctaatg
     3961 gcgctgagtg gcggtcacgg cttcgatgat attttcgtct ttgtgcctaa tgaaggactg
     4021 gtgactctcg cctcttcctt gctggcgaca gatggttgcc tgaatttctt cgccggaccg
     4081 caggataaac atttcagcgc gccaattaat ttctacgatg tgcattatgc atttacccac
     4141 tacgtgggca cgtcaggcgg caataccgac gacatgcgcg cggcggtcaa actgattgaa
     4201 gagaaaaaag tgcaggccgc aaaagtggta acacatattc ttgggctgaa tgccgcgggc
     4261 gaaaccacgc ttgaattgcc tgccgtcggc ggcggcaaaa agctggtgta taccgggaaa
     4321 tacctgccgc tgacgtcact cacgcagatt caggatcaag cactggcggc gattctggcg
     4381 cgtcatcagg ggatctggtc cggtgaggcg gagcaatatc tgctcactca tgcagaggca
     4441 atttcccatg attaatcgcg ctacttaact ctgcatgtcg ctggctggtc gccgcggcaa
     4501 cttcgcgacc tgatgtgcgg ctgattgctg aagcggccca atgtgcacga gaaggataaa
     4561 aaaacatcag cgccttaagg ggcgctcaaa ttggtggcaa agtacacatt gtttatgccg
     4621 gatgcggaat aaagccttat tcggcctaca taatctcgaa aattcaatat attgcagtta
     4681 tctggtaggc ctggtaagcg tagcgcatca ggcaatgctg tgtttgtcat cagtctcagc
     4741 tcccggtgca atacgtgaca tcgtgagcca tgtcagtaag tggttaaatt cgcagtggta
     4801 gcatgccttc cgtgatttct tactaaccgg agaagtaatg gaactgcttt tattgagtaa
     4861 ctcgacgctg ccgggtaaag cctggctgga acatgcactg ccgctaattg ctgaacagtt
     4921 gcagggtcgc cgctcagcgg tgtttatccc tttcgctggc gtaacgcaga cctgggatga
     4981 ttacacagcg aaaacggctg cggttctcgc tccgctgggt gtttctgtca ccggtattca
     5041 tagcgttgtc gatcccgttg ccgcgattga aaatgctgag atcgtgattg tcggcggcgg
     5101 gaatactttc cagttgctga aacagtgccg cgagcgcggg ctgctggcac caattactga
     5161 cgtggttaaa cgtggtgcgc tgtatattgg ctggagcgca ggcgctaacc ttgcttgccc
     5221 aaccattcgt accaccaacg atatgccgat tgtcgatccg caaggtttcg atgcgctaaa
     5281 tctgttcccg ttgcaaatca acccgcactt caccaacgcg ctgccggaag gccataaagg
     5341 tgaaacccgt gagcagcgta ttcgcgaact gctggtcgtc gcgccagaac tgacgattat
     5401 tggtctaccg gaagataact ggatcacagt gagtaaaggt cacgctacgc tgggtggccc
     5461 gaacaccacc tatgtgttta aggccggtga agaagcggtt ccgctggaag ctggtcaccg
     5521 tttttaagtg aattgccgga tgcatgatcc ggcatcttgt tcatagtgcc tgatgcgacg
     5581 cttgacgcgt cttatcaggc ctacaggatt cacaatatta atactcgtcc cgtccttcat
     5641 cttcatccga ctgttccagc acactgtaag ccaccgaaca aaacagcgag ttcaggcgct
     5701 gcatatctcc cagtaagcct aaatgtagcg aactggtttc aatgctctgc acgttttgct
     5761 gatgcaggcg atcgacgtgg gcgtgggaat agcggcgatt aagaatgcga aagcgatgct
     5821 tactacggcg caaacgacga gcgctggtga catcgccaga gaagaacacc gacattgcca
     5881 gctttaaatt actgagcaat tgctcataga gcgcatccag ttccttcaac ccatcaagcg
     5941 aaaatgcccg ccgtgctgcc agtgatttgt cagcaatttc gctgcccatg cgctcgacga
     6001 tatcggaggc ctgttcaagg ttgagcgaca tttcaataat ctccgcccag cggcgcgact
     6061 cttcctccgc cagctcttct tttggcatcc gcgccagata cagcttaatg gcggtgtaga
     6121 gaacgttgat atcatctgcc agcttacgca gctctttctc ctgccgtggc tcaccgtgca
     6181 tcactttatt caacccttcc atcatctgtt ccatggcgtc gccaatgcgc agggtttcgc
     6241 gcgcggcgtt cgccagagca agcgtgggcg tatccagcgc gctgacatcc agatgtttag
     6301 gccgtagctg ggtatccagt tccggttcat cgcgaataat cgttttgcaa aaccgtgcca
     6361 tcgggtcaac aaatggcagc atgaccaggc aacgtacaag gttgtagaag acgtggaaat
     6421 agatcaccag ttccgctttt ggcaatgaca gcttccccat tgtctctgcc agcaaatgga
     6481 caaacggcag gataatcagg ctacccacca gcttaaacag cagactaccc agcgcgacac
     6541 ggcgggctgc ggcattggcg gcactgttgt tgagcatcgc cagcaggccg gaaccgaggt
     6601 tagcaccaat caccagacag agcgccacgg ggaaggagat aatgcctgcg gcggtcagag
     6661 ccgcggtcag cagtacagca gcaaggctgg agtagctgat aatggcgaac atcgcgccaa
     6721 tcagcgcatc cagcagaata tcgccggtca gcgaggcaaa gatcacctga acgccgtttg
     6781 cctgggtgat cggcgttacg gcctgcacaa tcaactccag cgccagcaaa atcagcccaa
     6841 gaccaatacc gacgcggccc agttgcccgg cgcgtgactg tttgcgtccg aggaaaaaaa
     6901 tcacgccgat aaaaataagt aacggtgaca gccaggataa gtcgaaggtg agaatacgcg
     6961 ccattagcgc cgtcccgaca tctgccccca gcacaatgac cagagccggt gcgagggcta
     7021 ccagatcctg agcgacgaac gaggtcacca gcatggtggt ggcattactg ctctgtacca
     7081 gtgcggtaac gccgatcccc gcgcaaaagg cgagcggctt tttttcgacg ctccggctaa
     7141 ggacagtacg caaacgcgcg ccgaagacgc gcattacgcc ggttcgaaca atatgagtcc
     7201 cccagaccag cagggcgacg gcagaaagca ggtgaagcag cgttaacacg agttcaggtt
     7261 ctccttatcg ttatatgttc ctgaagcaat tgaatgacca aaggaccgga aacacgctga
     7321 ctggcatctt tactgtgcct tttgacattc gtttttccgg ctaagattat tccagcctga
     7381 ttccagtata agtgtttcat attaataaag agacagggca ctcattgagc gccctgtttg
     7441 ttgtaaggaa aagtgacaga tttgtgaatc agtccgcgtc ataccccaga ttcggtgcca
     7501 gccagcgctc aacatctgag acgctcatac ctttacggcg ggcatagtct tcaacctgat
     7561 cgcgctgaat ttgcgccacg gcgtagtact tgctgtccgg gtggctgaaa taccaacccg
     7621 aaaccgatgc accgggccac atggcgaaag attctgtgag tttcatgccg gtatgttttt
     7681 ccacttccag cagctcccag atggtggctt tttccgtatg ttccgggcag gccgggtagc
     7741 ccggtgccgg acggatgccc tggtagtttt cgcggatcag ttcttcgttg ctgaggttct
     7801 cgttcggcgc atagccccag tagactttac gcacacgctc atggagatac tccgcaaagg
     7861 cttcggctaa acggtcggca agcgctttca ccatgatttt gttgtaatca tcgtgctgcg
     7921 cttcaaaggc atcagccagt gcgtcctctt ccagcccgcc agtcacggca aatgcgccga
     7981 tgtaatctgc tttaccagaa agcttcggcg caacgaagtc agcgagacag tagttagcga
     8041 atcccgtttt ttcggtctgt tgacgcagat ggtggctgac attgatcaca tgggtgcgcg
     8101 tttcgtcacg gtagatttca atgtcatcgc ccacacggtt tgccgggaac aggcccacca
     8161 cgccacgcca cgcggattca gcgttttctc ggcgcttaat ttatccagca tgtcgttggc
     8221 gtctttaaac agccgctgcg cctcaacgcc caccacttca tcttccagaa tgcgcggata
     8281 cttcccggcc agcgaccagg tcataaagaa cggtgtccag tcgatgcaat tacgcagagt
     8341 ttcgatgctg gcttcaactt cctgcacgcc gagacggtgc gccaccggcg gcgtgtaagc
     8401 ctgccagtca aaagcgaagt cgttatcgcg cgccgcttcc agcgtgaccg gtggtgtgcg
     8461 cggtttcttg cgcccgtgct gaatacgtac ggtttcgtac tccttgcggg tacgagcgac
     8521 aaaatcatcg cgctgagtat cggaaagcag cgccgccacc acaccaacgg tgcgcgaggc
     8581 attctgcaca tacaccgtcg ggccgctgta gttctgctca attttcaccg ccgtgtgcgc
     8641 ttttgaggtg gtcgcgccac cgatcagtaa cggaatggtg aagccctgac gctccatctc
     8701 tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa ggccaatcaa
     8761 atcagcattc acttctttag cagtacggag aattttttcc gcaggcacca tcacgccgag
     8821 atcgacaatt tcgtagttgt tacattgcag caccacgcca acgatatttt taccgatatc
     8881 gtggacgtcg cccttcacgg tggcgatcac catcttgccg ttggttttgc cctgctcttt
     8941 gctggcttca ataaacggtt cgagatacgc caccgcctgt ttcatgacgc gcgccgattt
     9001 gaccacctgt ggcaggaaca ttttgccttc gccaaacaga tcgccgacca cgttcatgcc
     9061 gtccatcaac gggccttcaa tcacttcaat cgggcgcgta gcctgctggc gggcttcttc
     9121 ggtatcctgc tcgataaact cggtaatgcc tttgaccagc gagtattcca gacgtttatt
     9181 cacttcccac gagcgccact ccgcctgctg ggcgttatcg gtgtcgtcgg ttttgctgcc
     9241 gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcgtcacgac gattaagaat
     9301 cacatcttcc accgcgtcgc gcagttcagc gggtaggtcg tcgtaaatcg ccagttgccc
     9361 cgcgttgacg atccccatat ccatgccatt gcgaatagcg tagtagagga acactgcgtg
     9421 aatggcttcg cgcaccggat cgttgccacg gaatgagaaa gaaacgttag atacgccgcc
     9481 ggaaatcagc gcgtgcggca gttcgcgttt gatgtcttca cacgcgccga taaagtcctg
     9541 cgcgtagttg ttgtgctctt caataccggt ggcgaccgcg aagatgttcg ggtcgaagat
     9601 gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc gacggcaaat
     9661 ctcgattttc cgtgcgcgag tatcggcctg tccctgttca tcaaaggcca tcaccaccac
     9721 tgccgcaccg tagcgacgca acaatttcgc gtgatggata aaggcgtcga cgccctcttt
     9781 catcgagatt gagttaacaa tgcctttgcc ctgaatacac ttcagccctt tttcgataac
     9841 gtcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt caccggcaat
     9901 cagattgaga aaacgcacca tcgccgcttc ggcatcgagc atcccttcat ccatgttgat
     9961 atcgataatc tgcgcgccgt tttccacctg ctgacgcgcc acatcctgcg cctcgctgta
    10021 tttctcttct ttgatcaggc gcttgaactt agcagagccg gtgacgttgg tgcgttcacc
    10081 cacgttcaca aacaggctat cgtcgccaat gttcagcggc tccaggccag acaaacggca
    10141 ggctacggga atttccggca gtttgcgcgg cgctaatcct tctactgcac gactcatcgc
    10201 tgcaatatgt tgcggcgtgg tcccgcagca accaccgacg atattgagaa agcccgcttg
    10261 cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtatt caccaaaggc
    10321 gttaggcagc ccggcgttcg ggtgggcggt gacgtagcat tccgcaatcc gtgatagttc
    10381 ctgcacgtac tggcgcagtt catcgggccc cagcgcacag ttcaggccaa aggttagcgc
    10441 ttcggcgtgg cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga gcgtgcgccc
    10501 ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccca gcgcttcaaa
    10561 ctccgttttc accgcaaaaa ccgccgcttt ggcgttaagg gtgtcgaaaa cggtttcaat
    10621 caggatcaga tccgcaccac cttccaccag cgctttggtg gattcgcgat aggccgccac
    10681 cagctggtca aaagtgatgt tgcggaatgc cggatcgttg acgtccggag aaatagacgc
    10741 cgtgcggttg gttgggccga gaacaccggc aacgtagcgc ggtttctccg gcgtgcgcgc
    10801 ggtccactcg tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga tttccgccga
    10861 cagggattcc atctggtaat ccgccatcgc aatggtcgtg gagttgaagg tgttggtttc
    10921 gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca cttccggttt
    10981 gctgagcacc agcaggtcgt tgttgccttt gagatcgcat ggccagtcgg caaagcgttc
    11041 accacgaaaa tcggcttcgt tcagtcgata actctggatc atggtgccca taccgccgtc
    11101 cagcaccaga atacgttcat ttaactgcgc acgcagttgt tccactttgc tgctcacact
    11161 tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac tggcataaac
    11221 gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt aactcatcgg
    11281 atcagttcag caactattgc attagctaac aataaaaatg aaaatgattc ccacgataca
    11341 gaaaaaggag actgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa aacccgccgt
    11401 tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga aattactgga
    11461 gtggattgcc gaatccaatg gcagtgtggc actcacggaa ctggcgcaac aagccgggtt
    11521 acccaattcc acgacccacc gcctgctaac cacaatgcaa cagcagggtt tcgtgcgtca
    11581 ggttggcgaa ctgggacact gggcaatcgg cgcacatgcc tttatggtcg gcagcagctt
    11641 tctccagagt cgtaatttgt tagcaattgt tcaccctatc ctgcgcaatc taatggaaga
    11701 gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag cgattattat
    11761 cgaccaggta cagtgtacgc atctgatgcg aatgtccgcg cctatcggcg gtaaattgcc
    11821 gatgcacgct tccggtgcag gtaaagcctt tttagcccaa ctgagcgaag aacaggtgac
    11881 gaagctgctg caccgcaaag ggttacatgc ctatacccac gcaacgctgg tgtctcctgt
    11941 gcatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg acgatgagga
    12001 acatgcgctg gggctacgtt gccttgcagc gtgtattttc gatgagcacc gtgaaccgtt
    12061 tgccgcaatt tctatttccg gaccgatttc acgtattacc gatgaccgcg tgaccgagtt
    12121 tggcgcaatg gtgattaaag cggcgaagga agtgacactg gcgtacggtg gaatgcgctg
    12181 actttttctg gcgggcagag gcaatattct gccca
//
LOCUS       DAJTOR010000124        12194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTOR010000124 DAJTOR010000000
VERSION     DAJTOR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.124"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(156..494)
                     /gene="osmE"
                     /locus_tag="OO249_002993"
     CDS             complement(156..494)
                     /gene="osmE"
                     /locus_tag="OO249_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5637214.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            696..1523
                     /gene="nadE"
                     /locus_tag="OO249_002994"
     CDS             696..1523
                     /gene="nadE"
                     /locus_tag="OO249_002994"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5637215.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            1753..2640
                     /gene="cho"
                     /locus_tag="OO249_002995"
     CDS             1753..2640
                     /gene="cho"
                     /locus_tag="OO249_002995"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5637216.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(2600..3175)
                     /gene="ves"
                     /locus_tag="OO249_002996"
     CDS             complement(2600..3175)
                     /gene="ves"
                     /locus_tag="OO249_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5637217.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3378..3848)
                     /gene="spy"
                     /locus_tag="OO249_002997"
     CDS             complement(3378..3848)
                     /gene="spy"
                     /locus_tag="OO249_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5637218.1"
                     /translation="MRKLTALFVASTLALGAANLAHAAAPADAKPMMHHKGKFGPHQD
                     MMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIVSDTFDKAKAEAQIAK
                     MEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE"
     gene            complement(4178..5146)
                     /gene="astE"
                     /locus_tag="OO249_002998"
     CDS             complement(4178..5146)
                     /gene="astE"
                     /locus_tag="OO249_002998"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5637219.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5139..6482)
                     /gene="astB"
                     /locus_tag="OO249_002999"
     CDS             complement(5139..6482)
                     /gene="astB"
                     /locus_tag="OO249_002999"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR5637220.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6479..7957)
                     /gene="astD"
                     /locus_tag="OO249_003000"
     CDS             complement(6479..7957)
                     /gene="astD"
                     /locus_tag="OO249_003000"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5637221.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKLRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGRANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQLETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(7954..8988)
                     /gene="astA"
                     /locus_tag="OO249_003001"
     CDS             complement(7954..8988)
                     /gene="astA"
                     /locus_tag="OO249_003001"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5637222.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(8985..10205)
                     /gene="astC"
                     /locus_tag="OO249_003002"
     CDS             complement(8985..10205)
                     /gene="astC"
                     /locus_tag="OO249_003002"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR5637223.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIMAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGDFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            10651..11457
                     /gene="xthA"
                     /locus_tag="OO249_003003"
     CDS             10651..11457
                     /gene="xthA"
                     /locus_tag="OO249_003003"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5637224.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            11624..>12194
                     /locus_tag="OO249_003004"
     CDS             11624..>12194
                     /locus_tag="OO249_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5637225.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITL"
BASE COUNT         2945 a         3312 c         3008 g         2929 t
ORIGIN      
        1 tttctttgac cttataaatt gtctctcctt gacctgcggt tatgaccctc ttcacatttc
       61 gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa aaaaaccggc gcaatggccg
      121 gtttccgttg ttactcaagc tttcaaacga agtgattact tcgcagcctg tggatcagtg
      181 tcgtattcag cacaggtctg ataaccggag ttgatgacat gtccggtatc atctaacgcg
      241 acaaagtagg tttctgcttt accatcacgt tgacccagga tgtaggtctg gcaagtacca
      301 cgagcatgga tcatgctcac ttcagacgaa ggtttacccg caatttgtgc aacctgcgcc
      361 cggctcatgc cttttttgac gtctttcacc acaggctgta caaactggtc tttggtacga
      421 tcataagccg tacaacccgc cagcatggtt aataccgccg ctgcactcag aattcctgcc
      481 atattcttgt tcatattccg tcctcttgtt tatcagcgtg ttagataagc ctggaataca
      541 ttgggcgctt tttcaagccc gtgaacgaaa cggctccgct ttcagaggat tcctgtatga
      601 cgttttatcc accattcaac ccgctgtcgc ttgtcgtttc agtagcgacg ggttagcttt
      661 aaggaagttt tgtcttttct gtctggaggg gttcaatgac attgcaacaa caaataataa
      721 aggcgctggg cgcaaaaccg cagattaatg ctgaagagga aattcgtcgt agtgtcgatt
      781 ttctgaaaag ctacctgcaa acttatccgt tcattaaatc actggtgctc gggatcagcg
      841 gcggtcagga ctccacgctt gccggtaagc tgtgccagat ggcgattaat gagctgcgcc
      901 aggagaccgg caacgaatca ctgcaattta ttgccgtacg cctgccctat ggtgttcagg
      961 ccgacgaaca agattgccag gatgccattg cctttattca accggatcgc gtattaaccg
     1021 ttaatatcaa gggcgcggta ttggccagcg aacaggcgct gcgcgaagcg ggcattgaac
     1081 tgagcgattt tgtccgtggc aatgaaaaag cgcgtgagcg gatgaaagca caatatagca
     1141 ttgcgggtat gaccagcggt gtcgtggtgg gcaccgatca tgcagcagaa gctattaccg
     1201 gattcttcac taaatatggt gacggcggta cggacattaa tccgctgtat cgtctcaaca
     1261 aacgtcaggg taaacagtta ctggcggcat tagcttgccc ggaacacctt tataagaaag
     1321 cgccaacggc cgatctggaa gatgatcgcc cttctctgcc agatgaagtg gcactcggcg
     1381 tgacctatga caatatcgac gactatctgg aagggaaaaa cgtacctcaa caggtcgcca
     1441 gaacaataga gaactggtat ctgaaaaccg aacataaacg ccgtccgcca attaccgttt
     1501 tcgatgattt ctggaaaaag taataatttg cacatattgg attgtgcgaa aaagagtaat
     1561 ttgttcacgc cggatgcggc gtgaacgcct tattcgacct ataaaactat gcaaattcaa
     1621 tacattgcag gagtcgaata ggcctgacag gcgtagcacg tcagacggtg taacctttgt
     1681 catcgacccg cctctttttt aatcgcttcc cgcctgttac actggataga taaccagcat
     1741 tcggagtcaa cagtggtacg gcgtttaact tctccgcggc tcgaatttga agctgcggca
     1801 atttatgaat atcccgaaca tttacgttca ttccttaatg acttacccac ccgaccaggg
     1861 gtgtatctgt ttcatggtga aagtgacacc atgccgctct atatcggcaa aagcgttaac
     1921 atccgcagcc gcgtcctttc tcatttacgt accccggatg aagccgccat gctacggcaa
     1981 tcccgacgga tcagctggat atgtaccgcc ggtgaaatcg gcgctctgct ccttgaagcg
     2041 cgattaatca aagaacaaca gccgctgttt aataaacggt tgcgccgcaa tcgtcagctc
     2101 tgtgccctgc aattaaatga aaagcgcgtc gatgtggtgt atgccaaaga ggtggatttt
     2161 tcacgagccc ccaacctgtt tggcctgttt gccaataggc gcgcagcttt gcaagcattg
     2221 cagaccatcg ctgatgaaca aaaactttgt tatggcctgc tgggactgga accgttaagt
     2281 cgcggtcgtg catgttttcg ttcagcgcta aaacgttgcg ccggagcatg ctgcggtaaa
     2341 gagagccatg aggaacatgc gctacgcttg cgccagtctc tggagcgttt gcgggtggtg
     2401 tgttggcctt ggcaaggggc ggtggcgctg aaagaacagc acccggaaat gactcaatat
     2461 catattattc aaaactggct gtggctgggg gcggttaatt cgctggaaga cgcgacaacg
     2521 ttaattcgga cacccgccgg gtttgatcac gacggttata aaattctttg taagccgctg
     2581 ctttccggta actatgaaat tactgaactt gatccggcga atgaccagcg agccagttga
     2641 tttcactgaa caacagttta ccttgcggtt gtaaaagccg cagggtgtgc ctgccatcga
     2701 accagcacgc gccctggtcg gtagtgagca atttatcgcc cagttgccac gccccgttaa
     2761 tcacaaacac caccccgccg cgtgagccaa aggtagtaaa cgtacgttcg gcaattctga
     2821 ctttcgcctt acagacatcc aggcgtgtca tgatgttgaa atccatcgac atctgccctt
     2881 ccgtcagttt cgcttttacc acctggtccg ctgcaaaggc aaaaggctgt aacggtttta
     2941 aggtatggtt aaagcggtct gcgctttcaa ggagcatctc gccgccttcc agcaacgtca
     3001 ctatcctttc catgccggga aacaaagaaa actccccatt cgccgcgatg gatgcaatgc
     3061 tggcacgcca gtaaaaatca cgttttgccg gtgggaaagt gcaaatttcg cgcgtttcac
     3121 cggcagcatt tcgccacaga ttcaccgaca ttttacgcat atcaaagtat tccatactcg
     3181 ctcccggccc gttcttttca ttgttatcgt tcaggtaacc gtggatggtt atcggcaact
     3241 cgggtgattt acgttagtgg tgatcaggct ttttttacaa ctgtttgaga agagaaaaga
     3301 aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa
     3361 gttttttggc tgaaagatta ttcagcagtt gcaggcattt taccttttgc cgctggacgt
     3421 tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg cgtcaggatg
     3481 ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg ctgttcttcc
     3541 atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctgac aatgatgtca
     3601 tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg gcctttcatg
     3661 atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa catcatgtcc
     3721 tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc cggtgctgcg
     3781 gcatgggcca ggttagccgc gccaagagcc agggtagagg caacaaacag tgcagttaat
     3841 ttacgcatat tctatatcct tcctttcagt tatttattac ggctttctta agtagcgtgc
     3901 cgtgttgaca agattaactt tactgacttt agcgtcaatt aatcagagca acggtaaaac
     3961 aatgaaagtg taaaaaacac ttttgcgcca attatggaga aaaaaagaaa atttgatgga
     4021 gagtgatgag agaatattac aacacaatga ttttgcagag attatgaaga actatactgg
     4081 atgactggtg ataaataaag caaataacca ggattaatct gtattaattt ataagaaagc
     4141 aacttaatac ccgcagaatg atttctgcgg gtaagtatta gcttattttt tcgagcatta
     4201 atcccgcgcg taatcccaac gctaccaacg gattagggaa taacacatac tctacatcat
     4261 gggttacggt aaaacgttcc tctccgtcct gcgccagcaa tgttcctttc tcaaacggca
     4321 taaaattcag cgtgtcactt gccatatgca tttcgaagga cggcgagtga cgagtaattt
     4381 gcgaaaccac ccgataacgg agcggcggtg ttctcacgat accgacactc tcaccagata
     4441 gcagcgcagc aattgcgctg gcagttactg caaactggcg aagatcgttt tgcccaaagg
     4501 gcaacgcttt gccaagttcc agcgtacagg ccagcgcgcc aaaatgtctg gcgctgaaat
     4561 gggtaaacgt accaccaggt tcctgatgga acaccagcgc ctccagcccc gccgcaccca
     4621 gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg cggtaataca ccgaactgcg
     4681 gatgcaagga gccacgaatt gcggtatgta gatcaaggtg ccagcgcaca gattctttgc
     4741 cctggtcata aaaatcttcc aggcactgtt ccagttcgcg cgcccgacag gtttctccgc
     4801 tttcagcaaa tagctgccaa cgaccgccaa acattcgatt catatcgcta tggcaataac
     4861 gtttcccttg cttcagcgca ggaggattcc cgaggatcac cagcaaccgc caacgtaacg
     4921 ggatctcgcc gtgagatatc gcgccaagca acgcgtccag catttccaca ggtgccgtct
     4981 cattaccgtg gatccccgca gaaatcacca gcgcgccttg cggtggcgtt aatggcgtca
     5041 gttccagcac accatcgccc agccagcgcc agcgaacgcc gttgatttct cgctcggtga
     5101 taaccggttt tttacccgtt aaggtcagag caagaaaatt atccattgcc ccctccctcg
     5161 cgctggaacg gataaaccga accgagattc agtaattggc tcaatacatc cagtgcttcc
     5221 cgcccttcgc gcagcaattg cgggtcggcc agatcggcag cagtaagacg atcgcggtag
     5281 taacgatcca cccagtcatt gagcgcatta aacaaggtat cgttcatcat caccgccgga
     5341 ttcaccgccc ggcgttcttc ttctgtcaat accacccgca accgcaggca cgccgggccg
     5401 ccgccattcg ccatgctttc acgtaagtca aagactttta gttcgctaat cgggttgtca
     5461 gcggcaagga gttcattgag ataaccccat actccggcgt gttcccgaca ctcctgaggc
     5521 agcaccaaca tcatggaacc atcatcgcgg ctcaacagtt ggctgttaaa cagataggta
     5581 gacaccgcat ccgacacgga aacctgagtt gccggaactt ctatcgccat aaaaccgttc
     5641 acccgcgccc gcaggtttgc cagtaactgt gactggcgag cgaacgcctg ttggtggcaa
     5701 aacagcacct ggcggttact tacggcaatc acgtcattat gaaaaacacc ctggtcgata
     5761 acgtccgggt tttgctgggc gaaaatcacc tgttggggat tcacctgatt aagccttgcc
     5821 accgcctcgc tggcttcgcg agtctgtcgc gccggataac gggaaggccg ggtatcattg
     5881 ccttcttctc gcccgtagac aaaaagttgc atacccggct caccgtaatg accgccgaga
     5941 cgattgtggt ttgccgcccc ctcatcaccg agcaacgcta cctgcggcaa cgccgaatgg
     6001 acgctaaatt tctcttcgtc gttaaaaata gcttttaaca gcgattcagt gacgggcgct
     6061 tccagcgaac ggtgaaattt attgttcagg ttggcaacgg tgagatgcac tttgccatcc
     6121 agcgtatcgg cagatggcgc gatcgttgcc gcattggcta cccacattgg cgaagcggag
     6181 ctgacgctgg aaagccagtg cggtgcctgg cgtgcaactt tttccagtac ctgctcatcg
     6241 ctaccactga atcccaactg acgcagcacc ggaataaacg gacgctcgtg cggcgggatc
     6301 acggcctggg ggaatcctgc atcggcaagg gttttcattt tcagtaagcc ctgtttcgcc
     6361 gccagtcgcg ggttagacac ctgaaaacgg tgacgggtag aggcttcatt accaaacgac
     6421 aggccagcgt aatgatgcgt cagccctacc agcccgtcga aattaacttc ccaggcgttc
     6481 atcgcaccac ctcatcggaa aaatccagcc cggggttaag cgttgcgggc aaagttaacg
     6541 agtccgactc caggctcgcc ataggccatg cgcagtaatc tgcggcatac caggcgctgg
     6601 ggcgatggtt accggatgca ccaatgccgc cgaatggcgc ggtactggca gcaccagtaa
     6661 gcggtttgtt ccagttaaca atccccgccc gcgcctccag caacagttga tcgaattttt
     6721 cccgttcggg ggaaaccaga ccgcaagaga gtccgaagcg agtgttattc gccattagaa
     6781 tcgcttcctc gaaagaatca taacgccaga cgcgcaataa cggtccgaac acctcttcat
     6841 ctggtacgcc agcaacgcct gtcatttcaa tgatccccgg cgtcagcaac gatgtctcta
     6901 attgtaataa gcgcggcgca agcagggttc gtccgcccat cgcttccagt tgctgccagg
     6961 cagtaaccac ctgctgtgcg gcctgttcag aaatcagccc gccaataaac ggctgcggtt
     7021 cgtcatccca gttgcccggc gttaatcgct ggctgacggc aaccagacga gcaagaaacg
     7081 catcgccctg cgctccgctt ttgagcaata aacggcgggc gcaggtgcag cgttgcccgg
     7141 ctgtgacaaa cgccgactga atggtcagat ggacagccgc gtcgatatcc gccacctcat
     7201 cgataattag cggattatta ccgcccatct caagggcgag aattttctcc ggctgaccgg
     7261 agagctggcg atgcagctgg tagccggtat tggcgcgacc ggtaaacagc aaaccgtcga
     7321 gatcctccag cgcactcagc gcctgacccg tttcacgccc gccctgcacc aggttcagca
     7381 cgcccggcgg caagccagcc tgctgccata aacgcattac cgcttcgcca ctccacggtg
     7441 tcagttcgct gggtttaaag ataatggtgt tacctgccag caatgccgga acgatatgtc
     7501 cgttcggcaa atgaccaggg aaattatacg gcccaaacac cgccagcacg ccgtgcgggc
     7561 gatgtcgcag gctcgccgca ccgtccggca tttcactacg ctgctcgccg gtacgaacgt
     7621 gatacgcctt aattgatatc gcgattttat tgatcatcgc cgtcacttcg gttgccgctt
     7681 cccagcgcag cttacccgtt tctctggcaa taatcgcggt taattcggct ttattgcttt
     7741 ccagcaatcc ggcaaagcgt tcgacaacga cctgacgttc agccaatgag agccgcgccc
     7801 agcgcggaaa cgccgcacgg gctgcccgac aagcctgttc gacctgagcg gcatcggcat
     7861 cattgccttg ccataacacc tcgcccgata ccggattacg cttcacacgc gatgcgccct
     7921 ggcccgttat ccagtcaccg ttaatccata aagtcatgct gttttctcca ctgcgcacag
     7981 gcgcaccaga cgaacgcgat cccctgcgtg gcatttgagg gcatccagtt gtgcggcggt
     8041 taaaatcaaa cgctcggttg ccggatcggt acgcgccagc accacgcgga aatggtgata
     8101 attttcattg gcgaccaggc aggccggaaa atcgccctgc gcaggctgcc cttctgccac
     8161 ttccaccagc cgacttttac ggatggcgcg cacgcggtcg atgtcacact caagcgttgg
     8221 cccgccgtcg aagatgtcga tatagttgcg gtaacgaaaa ccttctttct ccagcaccgc
     8281 gcgggcaggc gcggtttgcg gatgtacctg accgatgacg tcctgggctt cctgggataa
     8341 aaagtgggta tagatcggat gtttcggcat cagttctgca ataaatgcct tttgcccggt
     8401 gccgcagaga aaatcggcgc ggctaaaatc catcgaaaag aagcgtttac cgaggctttg
     8461 ccagaacggt gaatagccgt gttcgtcaat caccccgcgc atttcagcaa ccactttgtc
     8521 attaaacttg tcgcgaaaag ccgccataaa cataaagcgc gatttcgaca gcaaatagcc
     8581 gttgccctct ttgcgccagt ccgggtcgag aaacagcgtg cacagctcgc tgctgccggt
     8641 gtgatcgtta ctaagaaaca gcgtcggcaa tgcgttatag acattcagct cttttgaggc
     8701 gtgaaccaac gtgccgaccc gatagttgtg ccagggatcg ttcagcccaa ccgccacctc
     8761 aatggcgcaa atccccgcca cggtgcctgt ctcgctatct tccagcacga acacatagcc
     8821 ctgctcgctt ttgggcagtt cgccttgcca ggttttgatt gccctttcga tacgcgcgga
     8881 aagcgtggct tcattagcgg gaagcgacgt caggccgccg cccgttttgc tggcaagctg
     8941 catcagcgcc gagacatctg agcgctcaac gggacggatg accatcatga tgaacctcgg
     9001 ctaacaaaat gttcgcaagc agctgcaaag cgatccagtc cggtcgtcac ctcttcttcg
     9061 ctgacattga gcgcaggcgc aaaacgcacc acgttgccac ccgcaatcag taccataacg
     9121 cctgctttcg ccgcctcctg agagatctgt ttcgcttgtc cggcgtaatc ggcattcagt
     9181 acacagccaa tcaacaaacc taagccgcga acttcactga acaaaccgta gcggtgatta
     9241 atggtattaa gacgctcaac aaaccagtcg tgacgctgtt taacgccatt aagcatctct
     9301 ggtgtgttga tgagctccag cactttgccc gccaccgccg aggccagcgg attgccgcca
     9361 taggtggtgc catgagtgcc aacggtcatc acgctggcgc actcttcggt tgttaacaac
     9421 gcaccgaccg ggaaatcgcc gcccagcgct ttggcggtag ttaacagatc aggtgtcacg
     9481 ccgtagtgca tataggcata cagttccccg gtgcgcccga cgccggtttg tacttcatca
     9541 aaaatcaaca gcgcattgtg gcggtcacac aattcacgca gaccttgtaa aaacgcgttg
     9601 ctggctggca ccacaccgcc ttccccctgg atgggttcga caatcaccgc acaggtggcg
     9661 tcgtcaatca gcgcgctggc agagttaata tcgttatatg cagcatgacg gatatctggt
     9721 ggcagtggcg caaaatcctg tgaataggct ggctgcccac ccgcactgac agtaaacagc
     9781 gtgcgaccat gaaacgcatt tttaaacgcc atgataccgc tcttatggct gccgtagcgg
     9841 tcgtgagcga atttacgcgc cagttttagc gccgcttcgt tggcttccgc accggagtta
     9901 caaaagaaga cgcgatcggc aaacgtggcg tcgatcaatt ttttcgccag tcgcagtacc
     9961 ggctcgttgg tgtaaccgtt gccggtatgc cagaacttac tcgcctgttc gttcagcgct
    10021 tcacgcagtt ccggatgcgc atggcccagc gcgttcaccg caatgccacc cgcgaagtcg
    10081 atatactctt tcccctgctg atcccacaag cgcgaacctt cgccacgtac cggtataaag
    10141 ggtgccggag cgtaaacagg tatcatccat tcatcaaagt tttcacgcgt aattggctga
    10201 gacatagcga cctctacagt aaataattcg ttatttatat gttaacaata agtaatgttt
    10261 gcgctgtaaa tgtagattgc agggttcgtg ccagccagtg ataaaagtgc ataaacggcg
    10321 gaggctaact ggaaatcaag gagttataac caaaccatat gcatttaaag tgcatataaa
    10381 gtgaatacgt ttgcgatgtg ggtgaataaa aagaataaaa aacgcaatgt tatgcagaag
    10441 taaaatataa ttctggaatt gtgatcattg acgaaattta ctggaaatta ctgcgccatt
    10501 ctgacgcagc gcgcaccaaa agcgggcatt ttttgcgcca tcgttgacat cattaacaac
    10561 catcgatcaa atcacttaac aacaggcggt aagcaacgcg aaattctgct accatccacg
    10621 cactctttat ctgaataaat ggcagcgact atgaaatttg tctcttttaa tatcaacggc
    10681 ctgcgcgcca gacctcacca gcttgaagcc atcgtcgaaa agcaccaacc ggatgtgatt
    10741 ggcctgcagg agacaaaagt tcatgacgat atgtttccgc tcgaagaggt ggcgaagctc
    10801 ggctacaacg tgttttatca cgggcagaaa ggccattatg gcgtggcgct gctgaccaaa
    10861 gagacgccga ttgccgtgcg tcgcggcttt cccggtgacg acgaagaggc gcagcggcgg
    10921 attattatgg cggaaatccc ctcactgctg ggtaatgtca ccgtgatcaa cggttacttc
    10981 ccgcagggtg aaagccgcga ccatccgata aaattcccgg caaaagcgca gttttatcag
    11041 aatctgcaaa actacctgga aaccgaactc aaacgtgata atccggtact gattatgggc
    11101 gatatgaata tcagccctac agatctggat atcggcattg gcgaagaaaa ccgtaagcgc
    11161 tggctgcgta ccggtaaatg ctctttcctg ccggaagagc gcgaatggat ggacaggctg
    11221 atgagctggg ggttggtcga taccttccgc catgctaatc cgcaaacagc agatcgtttc
    11281 tcatggtttg attaccgctc aaaaggtttt gacgataacc gtggtctgcg catcgacctg
    11341 ctgctcgcca gccaaccgct ggcagaatgt tgcgtagaaa ccggcatcga ctatgaaatt
    11401 cgcagcatgg aaaaaccgtc cgatcacgcc cccgtctggg cgaccttccg ccgctaattt
    11461 agcagctctc ctggctcaaa ctgggtcagg agaattaacc ttgagaaaaa tcaacaaact
    11521 gtcagtaatg atttgttgcc tgccgtcctt tgttataccg tcactgcgtt tttagttgtc
    11581 tgaccacttc tctattatca agtttgatat aggaaactcc acgatgaatg ctgagcgtaa
    11641 atttcttttc gcctgtctta tttttgcgct ggtcatttac gctatccacg ctttcggttt
    11701 attcgatctg ctcaccgatt taccccactt acagacactc attcgccaga gcggattgtt
    11761 cggttatagc ctctatattc tgttattcat cattgccacc ctctttctgt taccaggaag
    11821 catactggtg atcgctggag gaatagtttt tggcccgctc ttagggacac tactctcatt
    11881 aattgccgcc acgctggcct cctcgtgctc attcctgctg gcgcggtggc tgggacgtga
    11941 tttactgctg aaatacgtgg gccatagcca taccttccag gccattgaaa aaggcattgc
    12001 gcgtaacggt attgattttc ttattctgac ccgcttaatc ccgttgtttc cttacaatat
    12061 tcaaaattac gcttacggat taaccaccat cgccttctgg ccttataccc ttatttcggc
    12121 actcacgacc ctgcccggta ttgttattta taccgtgatg gcaagcgatc tcgccaatga
    12181 aggcattacg ctgc
//
LOCUS       DAJTOR010000125        12179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTOR010000125 DAJTOR010000000
VERSION     DAJTOR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.125"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(17..523)
                     /gene="mug"
                     /locus_tag="OO249_003005"
     CDS             complement(17..523)
                     /gene="mug"
                     /locus_tag="OO249_003005"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR5637226.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            648..723
                     /locus_tag="OO249_003006"
     tRNA            648..723
                     /locus_tag="OO249_003006"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:681..683,aa:Ile,seq:cat)
     gene            complement(777..1541)
                     /gene="nfeF"
                     /locus_tag="OO249_003007"
     CDS             complement(777..1541)
                     /gene="nfeF"
                     /locus_tag="OO249_003007"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR5637227.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1829..2452
                     /gene="nfeR"
                     /locus_tag="OO249_003008"
                     /gene_synonym="yqjI"
     CDS             1829..2452
                     /gene="nfeR"
                     /locus_tag="OO249_003008"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5637228.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2606..4126)
                     /gene="aer"
                     /locus_tag="OO249_003009"
     CDS             complement(2606..4126)
                     /gene="aer"
                     /locus_tag="OO249_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR5637229.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSIIASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDVSADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4543..5922
                     /gene="ygjG"
                     /locus_tag="OO249_003010"
     CDS             4543..5922
                     /gene="ygjG"
                     /locus_tag="OO249_003010"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5637230.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLLAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQSELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5964..6296)
                     /locus_tag="OO249_003011"
     CDS             complement(5964..6296)
                     /locus_tag="OO249_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR5637231.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6515..7498
                     /gene="ebgR"
                     /locus_tag="OO249_003012"
     CDS             6515..7498
                     /gene="ebgR"
                     /locus_tag="OO249_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5637232.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7682..10774
                     /gene="ebgA"
                     /locus_tag="OO249_003013"
     CDS             7682..10774
                     /gene="ebgA"
                     /locus_tag="OO249_003013"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5637233.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLGNLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            10771..11220
                     /locus_tag="OO249_003014"
     CDS             10771..11220
                     /locus_tag="OO249_003014"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5637234.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11283..>12179
                     /locus_tag="OO249_003015"
     CDS             11283..>12179
                     /locus_tag="OO249_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5637235.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGS"
BASE COUNT         2924 a         3290 c         3069 g         2896 t
ORIGIN      
        1 ttttcggtgg cgttttttat cgcccacgca ctaccagcgc ctggtccagc tcgcgatacg
       61 cttcaaccag tttctctagt gaaacgcgac ttaaaccgct gggatttggc agcacccaaa
      121 tctgcgtcga accaatggtg agcgtttgtt tcccccactg tgcaccgcgc tggctgaatc
      181 cctgttcata tgcttgtttg cccagaatcg ccaacgcctg cggctgataa tcttcaattt
      241 tttcaatcag cttacgccca cctgcgtgca gctcctgctt tgaaacttca ttggcttgca
      301 ccgttggacg gtctaccagt ttggtgacgc cacaacgata atccagcaga tgctgtgcct
      361 cctgcggctt caactgacgg tcggtaaacc cggcctgata tatcaccttc cagaagcgat
      421 ttgctggatg agcaaaggga aaaccagtcc cggcggatga aagcccaggg ttgataccgc
      481 aaaacacgac ccgtaaccct ggagccaaaa tatcctcaac catatttact cctttctaac
      541 atcaccagga aaagtataac ggattgaaaa tacattgttt ataaaaacag caggcgcacg
      601 gtaatggctg gattgcgaca cggagttact ttataatccg ctaccatggc cccttagctc
      661 agtggttaga gcaggcgact cataatcgct tggtcgctgg ttcaagtcca gcaggggcca
      721 ccagatatag caaaggctga cgagaaatcg tcagcctttt tctttttata tctcagttac
      781 tttgcgtgcc agtaagccgc tgcacgtacc cgctgtgggt catactgttc cgcttcaaag
      841 cggcggctta aattcttaac gactttacct tcgccggtta tccagatgaa gtaatcatcg
      901 gcagggattt gcatctgcgc cagacgcgca tctaccgcct gctcatcatg tgccagccat
      961 tcgatattaa aaccatcaag gtgcgcgaga taatcctgac aggcgttatc ccgcacgcta
     1021 accagcgcac taacttgcgg tttaacggca agtttgctca acgtttccag gcggcggcgc
     1081 agtgcgggca ttccggattc atcgcagaca tacagctgat acgcgtaatc ttccggcacc
     1141 accagcgaac cgcgcggacc agccaccgta agtttatcgc ccggttgcgc ctgcatcgcc
     1201 cagccgctgg cgaccccacc gtcgtgaata aagaaatcga tcgccagttc atggcgtagt
     1261 tcgtcataca gcggcgtata gtcacgcgac ggtgggcgtg gtccttccgg ccagacgatg
     1321 ccctcttccg ttaccgttgg cggcacaaag tgagcgtcag gttgaggaaa gaagagtttg
     1381 ctgtgatcgt caaagccgcg cgatgtaaaa ccgtccagcg cctcgccgcc gaggacaatg
     1441 cgctgaaaac cggcgctgat gcgctcaacg cgtaacacag tcagttcacg gaagcgcaga
     1501 tcattgcgaa cgcgctgcgg gtagcggggg gagttattca ttgttatcgc cttcgtgatg
     1561 gtaatcagat atatctaaat aattttcgta aatgataatg attgttaatc atgataaatg
     1621 caagcgatta gtagaactga tatgtctata gtctgataag acgaaccgcc tcttctcagg
     1681 catcattact caacgccgga tgcggcgtga acgccttatc cggcctacgt gtgagatgag
     1741 tccacattta aaaatcattt ttattcttgc aagagtgctc atatcagata taaattagat
     1801 atatctaatc aagaaaaagg aggctgatat gagccatcat cacgaagggt gttgtaaaca
     1861 tgaaggccag ccacgccatg agggctgctg caaaggtgag aagtcagaac acgagcactg
     1921 cggacacggt caccagcatg aacacggtca atgctgcggt ggtcgccacg gtcgcggcgg
     1981 cggtcgtcgg caacgtttct ttggtcacgg tgaattgcgt ctggtgattc tggatattct
     2041 ctcgcgcgat gacagccacg gttacgaatt gattaaagcg attgagaatc taacccaggg
     2101 gaattacacc ccaagcccgg gcgtcatcta cccaacgctg gattttctgc aggagcagtc
     2161 gctgattacc atccgcgaag aggaaggtgg taagaagcag attgcgctga ccgaacaagg
     2221 cgcgcagtgg ctggaagaaa accgcgaaca ggtggagatg attgaagaac gcatcaaagc
     2281 gcgttgcgtt ggcgcggcgc tgcgccagaa cccgcaaatg aagcgggcgc tggataattt
     2341 taaagcggtg ctggatttac gcgtcaacca gagcgatatc agtgatgcac aaataaaaaa
     2401 gatcattgcg gtgatcgacc gcgccgcttt tgatattacg caactggatt aatcgccgca
     2461 tccgccagtg gcgcggtgca attgccggat gcgacgcttg acgcgcctta tccggcctac
     2521 acccactaca caccccgcag gcctgataag atgcgccagc atcgcatcag gcattgtgct
     2581 ccaaccgccg gatccggcat accgattaat gcagcaccgt caccgcgtct tccagtcggc
     2641 tggcgcggtg tttcaccatc gccgacacct gcgcactctc ttccaccagc tcagcatttt
     2701 tctgggtgat caggttaagc tcatccactg cacgggtcag gctggaaagc ccatcggcct
     2761 gttccagcgt tgaatggcta atctgggcga tcaactgggt gacgtttttc acctgtgcca
     2821 caatatcttc catcgtccgt ccggcggcgt gtacctgctg cgaaccggat tgcaccttat
     2881 cagcactgac atcaatcagc ttgcgaatat cgttggcagc attagcgctg cggctggcta
     2941 aatgacgcac ttcccctgcc accactgcaa aacctttgcc ctgttcgccg gcacgcgccg
     3001 cttccaccgc cgcattcagg gccagaatat tggtctgaaa cgcaatatcg ttaatcagcg
     3061 aagtaatggt gccaatgcgc tgggtactgt cggcgatatc gtccattgtc ttgatcaccg
     3121 tggtcatcgc ctccccaccc tgcactgccg cattactggc gatgatagaa agtttatcgg
     3181 cagccgacgc cgtggcagag ttctgtttca ccgacgccgc catttggttc atggtcgcca
     3241 ccgtttgctg aacgttatca actgtctgct gggtatgttc gttcagttca tcggtgcctt
     3301 tcgccagcgt ctcactgcca tttctgacac tggacacctg gcttgagaca tcgttaatta
     3361 gccaacggca catcaggcca agctgcccta ccgcgcgtaa tgtcagcccc agctcatcgc
     3421 tgcgattcag gtgctcaaca ctattacgct ctccggtcgc caccttcagt gcctgacggg
     3481 cgacattttc tatcgggcgc acaatctgcc attcaaaaca agcgcttgcc aacaatacca
     3541 ctaacacaca gaggaaatac gtcaccaccg gggcagcaac aaaccaaagc atggccgcca
     3601 gcaagataaa catcagggtc atcactccac gcgcccgcca gcgaagcggt aatgaaggca
     3661 gtttacccag ccagccttta cgcaccacca ggcctttatg aatacgctta ctggtacgtc
     3721 cggcgttcag cgctttgtac agcggctcca ccgccgcaat ctcttcatcc gtcgcccggg
     3781 tacgaatcga catatagcca ctgatttttc cctcgcgcac catcggtacc gcattggccc
     3841 gcacccaata atggtcgcca tttttgcggc gatttttcac gatgccgctc cagggctccc
     3901 cttttttcag ggtgaaccac atatccgcaa acgccgcttt tggcatatcc gggtgacgca
     3961 ccatgttgtg cggctgccct tgtaactctt gcaaggtaaa gccgctcacc tgcacaaaag
     4021 tgtcattagc atgagtgata tagctttgca gatcggtagt ggacatcaga gtggtatcgt
     4081 ccgccagcgg ggtattttgc tgggtgacat acggatgaga agacatgttc gcgtcctatg
     4141 caggttatat ggttgttaac ttcttgtcag agtttatgtc ggccccgctg cggttatctt
     4201 taaccgatta atttgattta gatcgcaatt tgcgattaaa gcacaaatct aattccttga
     4261 tttaaaatac tttcactctg ttactatacg aaaacgttaa ttatcttgcc caaaatcagg
     4321 caattatcgc cctgaaaacg tgcatttgca cagcaatcat caaatccata cccgacaaaa
     4381 accgtgcaaa ataacaacga atgttaacaa atagcattaa atactgcgca aatgataacc
     4441 acatttgtgt ttatcccgat tttcgcgatc gcagccggag tggcgcaatc cctgcaatac
     4501 ttaaatcggt atcatgtgat acgcgagcct ccggagcata ttttgaacag gttaccttcg
     4561 agcgcatcgg ctttagcgtg cagcgcccac gccctgaatc tcattgagaa gcgaacgctg
     4621 gatcatgagg agatgaaagc acttaaccga gaggtgattg aatacttcaa agagcatgtc
     4681 aatccggggt ttttagagta tcgcaaatct gttaccgccg gcggggatta cggagccgta
     4741 gagtggcaag cgggaagttt aaatacgctt gtcgacaccc agggacagga gtttatcgac
     4801 tgcctgggag gttttggaat tttcaacgtg gggcaccgta atccagttgt ggtttccgcc
     4861 gtacagaatc aacttgcgaa acaaccgctg cacagccagg agctgctcga tccgttacgg
     4921 gcgatgttgg cgaaaaccct tgctgcgcta acgcccggta aactgaaata cagcttcttc
     4981 tgtaatagcg gcaccgagtc cgttgaagca gcgctgaagc tggcgaaagc ttaccagtca
     5041 ccgcgcggca agtttacttt tattgccacc agcggcgcgt tccacggtaa atcacttggc
     5101 gcgctgtcgg ccacggcgaa atcgaccttc cgcaaaccgt ttatgccgtt actgccgggc
     5161 ttccgtcatg tgccgtttgg caatatcgaa gccatgcgca cggctcttaa cgagtgcaaa
     5221 aaaaccggtg atgatgtggc tgcggtgatc ctcgaaccga ttcagggtga aggtggcgta
     5281 attctgccgc cgccgggcta tctcaccgcc gtacgtaagc tatgcgatga gttcggcgca
     5341 ctgatgatcc tcgatgaagt acaaacgggc atggggcgca cgggcaagat gttcgcctgc
     5401 gagcatgaga acgtacagcc ggatatcctc tgccttgcca aagcgctcgg cggcggcgtg
     5461 atgccgattg gcgcgaccat cgccactgaa gaggtgtttt cagttctgtt cgacaaccca
     5521 ttcctgcata ccaccacctt tggcggcaac ccgctggcct gtgcggcggc gctggcgacc
     5581 atcaatgtgt tgctggagca gaacttactg gctcaggctg agcaaaaagg cgatatgttg
     5641 ctggacggtt tccgtcaact ggcgcgggaa tatcccgatc tggtacagga agcgcgtggt
     5701 aaagggatgt tgatggcaat tgagtttgtt gataacgaaa tcggctataa ctttgccagc
     5761 gagatgttcc gccagcgcgt actggtggcc ggaacgctca ataacgccaa aacgatccgc
     5821 attgaaccgc cactgacact gaccattgaa cagtctgaac tggtgatcaa agcggcgcgt
     5881 aaggcgctgg cggccatgcg agtaagtgtc gaagaagcgt aatatcaaat cggatggcga
     5941 tacgacgtcg ccatccgatc tttttatacg acgcgtacgc ccgcaggcat catccgctcc
     6001 ggcgttaaca acacgctttc actgccgtca tcggtttccg cacacaacag catgcattcc
     6061 gacgtttcac cacgcatttt cgctttttgc agattgcata agaccaccac cgttttcccc
     6121 atcagctctt cttcgctgta gtacggcacc aggctggtca cggtttgcag tgttttttcc
     6181 cccacatcaa cctgtacgat gtacagcttg tcggcgtttt catggcgttt cacttccaca
     6241 atctttccga cgcgcatttc cagacgtgca aaatcagcgt aagccacggt ttccattgtt
     6301 tccttccttc ggataaaatt ttactaaact atagcaaaag tttttctcaa tcctgtaggc
     6361 taaaaatgga gaatgcaggc gtgatcacat tcgtgagccg ctgtgttacc gttacagcgt
     6421 caaagaaacg cgctttattt actgaaaaca ggtgacccga taagtacttc ctctacaatg
     6481 ggggcgcaca tcagggaaag taaaaaaggt aaacatggca acactaaaag acatcgcaat
     6541 cgaagctggc gtatccctgg cgacagtatc cagggtctta aatgacgatc cgacattgaa
     6601 tgtgaaagaa gagacgaaac atcgcattct cgagatcgcc gaaaagctgg agtacaagac
     6661 cagtagtgcc cgcaaactcc agacaggtgc agtcaaccaa caccatattc tggctatcta
     6721 cagctaccaa caggagctgg agatcaacga tccttactat ctggcgatcc gccacggcat
     6781 tgaaactcag tgcgaaaagc tgggcattga gctcaccaac tgttatgaac acagcggatt
     6841 accagacatt aaaaacgtca ccggtatttt aattgtcggc aaacccacgc ccgcccttcg
     6901 cgccgccgcc tgtgcgttga ccgacaatat ctgttttatc gactttcacg aacccggcag
     6961 cggttacgat gcggtggata tcgatctggc acgcatcagt aaagaaatca tcgacttcta
     7021 tatcaatcag ggcgttaatc gtattggttt tattggcggt gaagatgagc caggcaaagc
     7081 ggatattcgt gaggtcgcct ttgcggaata tggccgactg aaacaagtgg tacgcgaaga
     7141 ggatatctgg cgcggaggct tttccagttc gtcgggttat gaactggcaa aacagatgct
     7201 ggcgcaggaa gactatccga aggcactgtt tgttgcttcc gattccattg ctatcggcgt
     7261 actgcgggca attcatgaac gaggcctgaa catcccacag gatatttcgc ttatcagcgt
     7321 taacgatatc cccaccgcgc gatttacctt tccgccgctc tccaccgtgc gcatccattc
     7381 cgagatgatg ggaagtcagg gcgttaacct ggtgtatgaa aaagcccgcg atggtcgcgc
     7441 gctgccgctg ttagtcttcg ttcccagcaa attaaaactg cgcggcacga cccgttaaat
     7501 ccccttacac actgtccggc aatcgttttt gccggacagt gctgccgttt attttcgtga
     7561 tccagttaaa gtaaatgcat ttatctgcta ctttttagta aaaattttac taaactcccc
     7621 agcaattaca caaactacca tcaccatgaa tggttccgat ttctctctac cgggaggccc
     7681 tatgaatcgc tgggaaaaca ttcagctcac ccacgaaaac cgacttgcgc cgcgtgcgta
     7741 ctttttttca tatgattctg ttgcgcaagc gcgtaccttt gcccgcgaaa ccagcagcct
     7801 gtttctgccc ttaagcggtc agtggaattt ccactttttt gaccatccgc tgcaagtacc
     7861 agaagccttc acctctgagt taatggctga ctgggggcat attaccgtcc ccgccatgtg
     7921 gcaaatggaa ggtcacggca aactgcaata taccgacgaa ggttttccgt tccccatcga
     7981 tgtgccgttt gtccccagcg ataacccaac cggtgcctat caacgtattt tcaccctcag
     8041 cgacggctgg cagggtaaac agacgctgat taaatttgac ggcgtcgaaa cctattttga
     8101 agtctatgtt aacggtcagt atgtgggttt cagcaagggc agtcgcctga ccgcagagtt
     8161 tgacatcagc gcgatggtta aaaccggcga caacctgttg tgtgtgcgcg tgatgcagtg
     8221 ggcggactct acctacgtgg aagaccagga tatgtggtgg tcagcgggga tcttccgcga
     8281 tgtttatctg gtcggaaaac acctaacgca tattaacgat ttcactgtgc gtaccgactt
     8341 tgacgaagcc tattgcgatg ccacgctttc ctgcgaagtg gtgctgggaa atctcgccgc
     8401 ctcccctgtc gtcacgacgc tggaatatac cctgtttgat ggcgaacgcg tggtgcacag
     8461 cagcgccatt gatcatttgg caattgaaaa actgaccagc gccagctttg cttttactgt
     8521 cgaacagccg cagcaatggt cagcagaatc cccttatctt taccatctgg tcatgacgct
     8581 gaaagacgcc aacggcaacg ttctggaagt ggtgccacaa cgcgttggct tccgtgatat
     8641 caaagtgcgc gacggtctgt tctggatcaa taaccgttat gtgatgctgc acggcgtcaa
     8701 ccgtcacgac aacgatcatc gcaaaggccg cgccgttgga atggatcgcg tcgagaaaga
     8761 tctccagttg atgaagcagc acaatatcaa ctccgtgcgt accgctcact acccgaacga
     8821 tccgcgtttt tacgaactgt gtgatatcta cggcctgttt gtgatggcgg aaaccgacgt
     8881 cgaatcgcac ggctttgcta atgtcggcga tatcagccgt attaccgacg atccgcagtg
     8941 ggaaaaagtc tacgtcgagc gcattgttcg ccatatccac gcgcagaaaa accatccgtc
     9001 gatcatcatc tggtcgctgg gcaatgaatc cggctatggc tgtaacatcc gcgcgatgta
     9061 ccatgcggcg aaagcgctgg atgacacgcg actggtgcat tacgaagaag atcgcgatgc
     9121 tgaagtggtc gatattattt ccaccatgta cacccgcgtg ccgctgatga atgagtttgg
     9181 tgaatacccg catccgaagc cgcgcatcat ctgtgaatat gctcatgcga tggggaacgg
     9241 accgggcggg ctgacggagt accagaacgt cttctataag cacgattgca ttcagggtca
     9301 ttatgtctgg gagtggtgcg accacgggat ccaggcacag gacgaccacg gcaatgtctg
     9361 gtataaattc ggcggcgact acggcgacta tcccaacaac tataacttct gtcttgatgg
     9421 tttgatctat tccgatcaga cgccgggacc gggcctgaaa gagtacaaac aggttatcgc
     9481 gccggtaaaa atccacgcgc gggatctgac tcgcggcgag ttgaaagtcg aaaataaact
     9541 gtggtttacc acgcttgatg actacaccct gcacgcagag gtgcgcgccg aaggtgaaac
     9601 gctcgcgacg cagcagatta aactgcgcga cgttgcgccg aacagcgaag cccccttgca
     9661 gatcacgctg ccgcagctgg acgcccgcga agcgttcctc aacattacgg tgaccaaaga
     9721 ttcccgcacc cgctacagcg aagccgggca ttctatcgcc acttatcagt tcccgctgaa
     9781 ggaaaacacc gcgcagccag tgcctttcgc accaaataat gcgcgtccgc tgacgctgga
     9841 agacgatcgt ttgagctgca ccgttcgcgg ctacaacttc gcgatcacct tctcaaaaat
     9901 gagtggcaaa ccgacatcct ggcaggtaaa tggcgagtcg ctgctgaccc gcgagccaaa
     9961 gatcaacttc ttcaagccga tgatcgacaa ccacaagcag gagtacgaag ggttgtggca
    10021 gccgaatcat ttgcagatca tgcaggaaca tctgcgcgac tttgccgtag aacagagcga
    10081 tggtgaagtg ttgatcatca gccgcacagt tattgcaccg ccggtgtttg acttcgggat
    10141 gcgctgtacc tacatctggc gcatcgctgc cgatggccag gttaacgtgg cgctttccgg
    10201 cgagcgttac ggcgactatc cgcacatcat tccgtgcatc ggtttcacca tggggattaa
    10261 cggcgaatac gatcaggtgg cgtattacgg tcgtggaccg ggcgaaaact acgccgacag
    10321 ccagcaggct aacatcatcg atatctggcg cagcaccgtc gatgccatgt tcgagaacta
    10381 tcccttcccg cagaacaacg gcaaccgtca gcatgtccgc tggacggcac tgactaaccg
    10441 ccacggcaac ggtctgctgg tggttccgca gcgcccaatt aacttcagcg cctggcacta
    10501 tacccaggaa aacatccacg ctgcccagca ctgtaacgag ctgcagcgca gtgatgacat
    10561 caccctgaac ctcgaccacc agctacttgg cctcggctcc aactcctggg gcagcgaggt
    10621 gctggactcc tggcgcgtct ggttccgtga cttcagctac ggctttacgt tgctgccggt
    10681 ttctggcgga gaagctaccg cgcaaagtct ggcgtcgtat gagttcggcg cagggttctt
    10741 ttccacgaat ttgcacagcg agaataagca atgaggatca tcgataactt agaacagttc
    10801 cgccagattt acgcctctag caagaagtgg caacgctgcg ttgaagcgat tgaaaatatc
    10861 gacaacattc agcctggcgt cgcccactcc atcggtgact cattgactta ccgcgtggag
    10921 acagactccg cgaccgatgc gctatttacc gggcatcgac gctattttga agtgcattac
    10981 tacctgcaag ggcagcaaaa aattgaatat gcgccgaaag agacattaca ggtagtggaa
    11041 tattatcgtg atgaaactga ccgtgaatat ttaaaaggct gcggagaaac cgttgaggtc
    11101 catgaagggc aaatcgttat ttgcgatatc catgaagcgt atcggtttat ctgcaataac
    11161 gcggtcaaaa aagtggttct caaagtcacc atcgaagatg gttatttcca taacaaataa
    11221 caactacggc ggcaaaagga gtttgccgcc accgctaccc tacacatttt cggagatgtg
    11281 ttatgtctga taccaaacgt aatacaatcg gcaaattcgg cttgctctcg ctgacttttg
    11341 ctgccgttta cagctttaac aacgttatca ataataatat tgagcttgga ctggcctcgg
    11401 caccgatgtt tttcctcgcg acgatttttt attttattcc cttctgtctg atcatcgcag
    11461 aatttgtttc gttaaataaa aactcagaag ccggtgtcta cgcgtgggta aaaagttcgc
    11521 tgggcggacg ttgggcattt attactgcct atacctactg gttcgtaaac ctgttctttt
    11581 tcacctcact gttgccgcgc gttattgctt atgcttcgta tgccttcctc ggatacgaat
    11641 atattatgac gccggttgcc accaccatta tcagtatggt gctgttcgcc ttctccacct
    11701 gggtttccac caacggggcg aaaatgttgg ggccaattac ctccgtcact tcaacgctga
    11761 tgctgctgtt aacgctctcc tacattttac tggcaggtac ggcgctggtt ggcggcgtac
    11821 agcctgctga ccccatcacc gttgacgcga tgatcccgaa cttcaactgg gcgttcctcg
    11881 gcgttaccac ctggatcttt atggccgcag gtggcgcgga gtccgtcgct gtgtacgtta
    11941 acgacgtcaa aggcggttcg aaatcgttcg ttaaagtgat catcctcgcc gggattttta
    12001 tcggcgtact gtattccgtc tcctcggtgc tgattaacgt cttcgtcagc agcaaagagt
    12061 tgaaatttac cggcggatcg gtgcaggtat tccatggcat ggcggcgtat tttggtctac
    12121 cggaagcgtt gatgaatcgc tttgtcggtc tggtgtcctt taccgcgatg ttcggttcc
//
LOCUS       DAJTOR010000126        11956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTOR010000126 DAJTOR010000000
VERSION     DAJTOR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.126"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..413)
                     /locus_tag="OO249_003016"
     CDS             complement(<1..413)
                     /locus_tag="OO249_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5637236.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYVLTTQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVTKAGYIALAPDGLSSVG"
     gene            569..1609
                     /gene="gpr"
                     /locus_tag="OO249_003017"
     CDS             569..1609
                     /gene="gpr"
                     /locus_tag="OO249_003017"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5637237.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1649..2143)
                     /locus_tag="OO249_003018"
     CDS             complement(1649..2143)
                     /locus_tag="OO249_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5637238.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2334..3218
                     /gene="yghA"
                     /locus_tag="OO249_003019"
     CDS             2334..3218
                     /gene="yghA"
                     /locus_tag="OO249_003019"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5637239.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3490..3915)
                     /gene="exbD"
                     /locus_tag="OO249_003020"
     CDS             complement(3490..3915)
                     /gene="exbD"
                     /locus_tag="OO249_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5637240.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3922..4656)
                     /gene="exbB"
                     /locus_tag="OO249_003021"
     CDS             complement(3922..4656)
                     /gene="exbB"
                     /locus_tag="OO249_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5637241.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4908..6095
                     /gene="metC"
                     /locus_tag="OO249_003022"
     CDS             4908..6095
                     /gene="metC"
                     /locus_tag="OO249_003022"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5637242.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6235..6894
                     /locus_tag="OO249_003023"
     CDS             6235..6894
                     /locus_tag="OO249_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5637243.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6934..7833)
                     /gene="yqhC"
                     /locus_tag="OO249_003024"
     CDS             complement(6934..7833)
                     /gene="yqhC"
                     /locus_tag="OO249_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5637244.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8027..9190
                     /gene="yqhD"
                     /locus_tag="OO249_003025"
     CDS             8027..9190
                     /gene="yqhD"
                     /locus_tag="OO249_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5637245.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAADNYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9295..10122
                     /gene="dkgA"
                     /locus_tag="OO249_003026"
     CDS             9295..10122
                     /gene="dkgA"
                     /locus_tag="OO249_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5637246.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            10322..11248
                     /locus_tag="OO249_003027"
     CDS             10322..11248
                     /locus_tag="OO249_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5637247.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11299..11556
                     /gene="yqhH"
                     /locus_tag="OO249_003028"
     CDS             11299..11556
                     /gene="yqhH"
                     /locus_tag="OO249_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5637248.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
BASE COUNT         3091 a         3040 c         2980 g         2845 t
ORIGIN      
        1 ccaacggaac ttaagccgtc aggtgccagg gcgatatacc ccgccttcgt cactcgccgt
       61 gccacatctt cgatatacgg attcagtcca cgattctcat gcaccaccac cacggctggc
      121 gttttgccgc tcatttttgc gggtttcacc agataacccc gtacctcgcc gtgaccattt
      181 ggcgaaggat acgtgatgta ctcagcaaca atctccgggt cggtaaactc tacctgagtc
      241 gtcagcacat aatttggctt gagcaaatca aacaacgcta atgccgtcat cccgcccacc
      301 gcatacttcg ccgcaaggtt gaggaactca cgtttcgaaa ttttcccgtg agcgtaatag
      361 tcgtagtaat ccaacaactc ttgtgggaaa tctttggcgg ttaaacgcgg catcgttgca
      421 ttcctcaact ggtgtttttt aagcaaagca taagcgcgtg tttttgccca gtttttcgtc
      481 actctgtgag ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt
      541 cgaaattgaa cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg
      601 gcagatgcag taccgctatt gcggaaaaag tggtttacgc ctgcccgcgt tatcgctcgg
      661 tttatggcac aatttcggtc acgttaacgc gctggaatca cagcgtgcaa tcctgcgtaa
      721 agcgtttgat ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg
      781 aagcgcagaa gagaactttg gtcgcctgct gcgggaggat tttgccgctt atcgcgatga
      841 actgattatc tctaccaagg ctggctacga tatgtggccc ggcccttacg gctctggcgg
      901 ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta
      961 tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc
     1021 tgcgctggct catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc
     1081 gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgcttttaat
     1141 tcatcaacct tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc tgctggatac
     1201 cctgcaaaat aacggcgtgg gctgtattgc ctttactcct ctggctcagg gattgctgac
     1261 cggaaaatat ctcaacggca ttccgcaaga ttcacggatg catcgtgaag ggaataaagt
     1321 tcgtggtctg acgccgaaaa tgcttaccga agccaacctc aacagcctgc gcttattgaa
     1381 tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa
     1441 agatgatcgc gtgacgtcgg tattgattgg tgtcagccgc gcggagcaac tagaggagaa
     1501 cgtgcaggcg ctgaataatc tgacatttag caccgaggag ctggcgcaga ttgatcagca
     1561 tatcgccgat ggcgagctga atctgtggca ggcgtcttcc gataaatgac ctgttaataa
     1621 cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat
     1681 agcccatcac aaatgcagag agcacaaacg tcagatggat aatgacgtac cacatcagtt
     1741 tgttatccgg gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg
     1801 ccacaatcga cgctgctact ttgtttttca gcgacgttgc gtccattttc cccagccagt
     1861 tcagcttctc tttgttctcg gagatatcca gctgcgagac gaaattctca taaccggaaa
     1921 acatcaccat caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga
     1981 ggatcaaatc tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga
     2041 agaacttcag cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca
     2101 gccagcgaga agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag
     2161 ttaaaacggg caacagtata tcgcaattga gtaaacagca cgcaacagca aatcaacata
     2221 tacgttaaac ctttgtcgca taattctgag gtttagggat cttcctggtt cccttatcaa
     2281 cgcatcgaga gcgtctaccc ttaaaagacg ttgaacctga agggagaaaa acaatgtctc
     2341 atttaaaaga cccgactacg cagtattaca ctggtgaata tcccaaacag aaacaaccga
     2401 cgccaggcat ccaggcgaag atgacaccgg taccggattg cggcgagaaa acctatgttg
     2461 gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc
     2521 gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg
     2581 tggaagaaga agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg
     2641 ttctgctgcc aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc
     2701 acaaggcgtt aggcgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc
     2761 cggatattgc agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg
     2821 cgctgttctg gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtatcatca
     2881 ccacttcgtc aatccaggca taccagccaa gtccgcattt actggactat gcggctacga
     2941 aggcggcgat tctgaactac agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc
     3001 gggtgaatat tgtcgcgcca ggccctatct ggacggcact gcaaatttcc ggcggacaaa
     3061 cgcaggataa gatcccgcag tttggtcagc aaacgccgat gaagcgtgcg gggcaaccgg
     3121 cggaactggc ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag
     3181 aagtgcacgg tgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag
     3241 caaccgggca tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct
     3301 acaaacgcat gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc
     3361 atcaggcatt gagcacctaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaacg
     3421 cacgaagatt caacatgttg caatagattt tgtaggcctg atgagcgtag cgcatcaggc
     3481 aattctactt tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc
     3541 cgcctgatgc agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttatccgc
     3601 tcggaagaag atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat
     3661 tgtttcatcg gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag
     3721 ataaaccggt ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt
     3781 cacatctacc gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac
     3841 gtcgataaac ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt
     3901 aagatgcatt gccatcggat attatcctgc gcgtaatttt tgtgcgacac gaaccggatg
     3961 cgcagcggcg ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc
     4021 aacatcaccc agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac
     4081 gaccgcagga atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc
     4141 cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat
     4201 gatgccccag accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag
     4261 atagccgtta ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa
     4321 gctggtacgt tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt
     4381 ctgcgcttca ttgaacaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc
     4441 gttggcctgg tttaaggaac gcgcttcagc cagcagttgc tgctcgcgct ttaggcgacg
     4501 cttctgattg aagaactcta cgctcttact gaagaagatt gcccaggtga ctacggaggc
     4561 caaaataagc ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca
     4621 gacggaaagg tctgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca
     4681 caaaatctgc gcataataat atcaaaacga cgtcgaattg atagtcattc tcattactat
     4741 ttgcatactg ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca
     4801 gcacgccatt tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata
     4861 ttcatgctag tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa
     4921 agcttgatac tcaactggtg aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa
     4981 atagcgtgat tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg
     5041 cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt
     5101 tttccttaca acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct
     5161 gcggggcggc agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt
     5221 tgatgaccaa cactgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac
     5281 tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc
     5341 agccaaacac taaaatcgtg tttctggaat cgccaggctc catcaccatg gaagtccacg
     5401 acgttccggc gattgttgcc gccgtacgca gtgtggtgcc ggatgccatc attatgatcg
     5461 acaacacctg ggcagccggt gtgctgttta aggcactgga ttttggcatc gatgtttcta
     5521 ttcaagccgc caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt
     5581 gcaatgcccg ttgctgggag cagctacggg aaaacgccta tctgatgggc cagatggtcg
     5641 atgccgatac cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc
     5701 aacatcatga aagcagtctg aaagtggctg aatggctggc agaacatccg caagtagcgc
     5761 gagttaacca ccctgctctg cctggcagta aaggacacga attctggaaa cgagacttta
     5821 caggtagcag cgggctgttt tcctttgtgc ttaagaaaaa actcagtaat gaagagctgg
     5881 cgaactatct ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat
     5941 cgttgatcct ggcgaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg
     6001 attttagcgg gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg
     6061 ccgatctgga cgccggtttt gcgcgaattg tataacattg ccacttttgg acaattttgc
     6121 agacatttaa ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga
     6181 atcaggagta caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct
     6241 gttattcaag atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct
     6301 catattgtta gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg
     6361 ctgcccgcct catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc
     6421 cagggggtta tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc
     6481 tgctggctaa gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg
     6541 ctggcacagc ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt
     6601 ctgctggcgc tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg
     6661 gcgggaattt ccggtctacc aaaccgccgc ttccagtttt tcaactggtt aagtggattg
     6721 ctgtgggtca gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa
     6781 cgccatgaag atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct
     6841 ggcttgttag gcatgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt
     6901 tccccgttcc cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct
     6961 tgccgcatct tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga
     7021 tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat
     7081 catcatccgc gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt
     7141 gacagactta aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg
     7201 ctcgacgctc aggttttcgg tgtatttatt ctcaatccgt ttcagcacgc ggctaatcag
     7261 actgaagtga gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag
     7321 cacgtagtac agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg
     7381 ctccatcacg tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga
     7441 gttaatcccg ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa
     7501 cagttcctgt aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga
     7561 cgtggcatag gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc
     7621 atcataacga aacacgcgtt cattgatata accgatttta tgcccggaaa agagaattat
     7681 gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc gtctcaccat acaacaaacg
     7741 cacatcgggc aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc
     7801 cgccagcaag cggcaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg
     7861 gcaatttgat tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga
     7921 cattggcaga aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg
     7981 cagatcgttc tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa
     8041 tctgcacacc ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca
     8101 aattcctcac gatgctcgcg tattgattac ctacggtggc ggcagcgtga aaaaaaccgg
     8161 cgttctcgat caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat
     8221 tgagccaaac ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa
     8281 agtgactttc ctgctggcgg ttggcggcgg ttctgtactg gacggcacca aatttatcgc
     8341 cgcagcggat aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa
     8401 agagattaaa agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga
     8461 atccaacgca ggcgcggtga tctcccgtaa aaccactggc gacaagcagg cgttccattc
     8521 tgcccatgtt cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc
     8581 gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac
     8641 caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctgat
     8701 cgaagatggt ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat
     8761 gtgggcggcg acgcaggcgc tgaacggttt gattggcgct ggcgtaccgc aggactgggc
     8821 aacgcatatg ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact
     8881 ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgat accaagcgcg ctaagctgct
     8941 gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc
     9001 cgcgattgcc gcaacccgca atttctttga gcaattaggc gtgccgaccc acctctccga
     9061 ctacggtctg gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat
     9121 gacccaactg ggcgaaaatc atgacattac gttggatgtc agccgccgta tatacgaagc
     9181 cgcccgctaa gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag
     9241 ttcacaacac ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct
     9301 aatccaaccg ttattaagct acaggatggc aatgtcatgc cccagctggg actgggcgtc
     9361 tggcaagcaa gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat
     9421 cgctcgattg ataccgccgc ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa
     9481 aatgcctcag tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac
     9541 aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac
     9601 ctctacttaa tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc
     9661 atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc
     9721 catcacctgc aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa
     9781 cttcatccgc tgatgcaaca acgccagcta cacgcctgga acgcgacaca caaaatccag
     9841 accgaatcct ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt
     9901 cgcgatctgg cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg
     9961 gatagcggcc tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt
    10021 gatgtctggg atttccgtct cgacaaagac gaactcggcg aaattgcaaa actcgatcag
    10081 ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct aacatgcaaa ttctcccggt
    10141 ggcggtaatg ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc
    10201 gttgttactc ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga
    10261 agctcttaaa agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca
    10321 gatgaaaata atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcgcagcc
    10381 tccttcacag accgtagaac aaacagtccg gcagatttat cagaactata aatcagatgc
    10441 cagtacgcct tatttcggtg aaaccggaga gcgggcgata acttcagcgc gtattcaaca
    10501 ggcgcttacc ctgaacgaca atcttacgct gccgggcaat attggctggc tggattatga
    10561 tccggtttgt gattgtcagg attttggcga tctggtgcta gaaagcgttg cgataaccca
    10621 aactgacgcc gatcatgccg atgccgttgt gcgctttcgt atctttaaag atgataaaga
    10681 aaagaccacg cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgacgatat
    10741 tgtcagcaat catggcagtg tcttacaagc agttaatagc gagaatgaaa aaacgctggc
    10801 cgctttagct tcgttgcaaa aagaacagcc ggaagccttt gttgccgaac tctttgaaca
    10861 tattgctgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt
    10921 taatgccttc tataaaacca ccttcaagac ggccaataat cccgatgaag atatgcaaat
    10981 agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc tattttcacg
    11041 cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc atgttcgttt
    11101 tacgctgacc aatggcaaca acgaagagca agaactggtt ttacagcggc gcgaaggcaa
    11161 gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc
    11221 aaaaactgcc gccagattaa agcaatgagc tgaattaaat aacaattagc cggaacaata
    11281 aataaaaggg aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc
    11341 tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc agctggcgag caatgtgcaa
    11401 acattaaatg ccaaaatcgc ccggcttgag caggatatga aagcactacg cccacaaatc
    11461 tatgctgcca aatccgaagc taacagagcc aatacgcgtc ttgatgcaca ggactatttt
    11521 gattgcctgc gctgcttgcg tatgtacgca gaatgataaa aaaatccccg gcagcatgtc
    11581 agttgccggg ttataggctg tttgctaaaa aaagccaaac ataaggacat gattatgcga
    11641 cgctggcaca cctgtttttt tattctgctg ttctgtggct tcagcggaaa cgtgttgtgc
    11701 aaagaatcga ttaatgaagc gatagaacgt ttgtaccaac cattgaccag taatgacgat
    11761 ataccaataa ccgatgaatt cagtctcgat ttgcgaagta atatgagcgc tactcctggc
    11821 aaaatgacac cctggctgaa taataatctt ttgtgtcgct gtgtcgatga cgctttcgtt
    11881 tctgcagaaa taatccaaag cacacaccta aatggcggcc gaacgcatgt actgttgcag
    11941 ctggttatca aacaaa
//
LOCUS       DAJTOR010000127        11926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTOR010000127 DAJTOR010000000
VERSION     DAJTOR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.127"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            216..1325
                     /gene="livK"
                     /locus_tag="OO249_003029"
     CDS             216..1325
                     /gene="livK"
                     /locus_tag="OO249_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5637249.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1373..2299
                     /gene="livH"
                     /locus_tag="OO249_003030"
     CDS             1373..2299
                     /gene="livH"
                     /locus_tag="OO249_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5637250.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2296..3573
                     /gene="livM"
                     /locus_tag="OO249_003031"
     CDS             2296..3573
                     /gene="livM"
                     /locus_tag="OO249_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5637251.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3570..4337
                     /gene="livG"
                     /locus_tag="OO249_003032"
     CDS             3570..4337
                     /gene="livG"
                     /locus_tag="OO249_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5637252.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4339..5052
                     /gene="livF"
                     /locus_tag="OO249_003033"
     CDS             4339..5052
                     /gene="livF"
                     /locus_tag="OO249_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5637253.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRAASGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5449..6120
                     /locus_tag="OO249_003034"
     CDS             5449..6120
                     /locus_tag="OO249_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR5637254.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENRHLPGDRRRHVRRAGSGH
                     HDADL"
     gene            6050..6253
                     /locus_tag="OO249_003035"
                     /pseudo
     CDS             6050..6253
                     /locus_tag="OO249_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
     gene            6250..7095
                     /gene="ugpE"
                     /locus_tag="OO249_003036"
     CDS             6250..7095
                     /gene="ugpE"
                     /locus_tag="OO249_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5637255.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATTTF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            7097..8167
                     /gene="ugpC"
                     /locus_tag="OO249_003037"
     CDS             7097..8167
                     /gene="ugpC"
                     /locus_tag="OO249_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR5637256.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIVLSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            8164..8907
                     /gene="ugpQ"
                     /locus_tag="OO249_003038"
     CDS             8164..8907
                     /gene="ugpQ"
                     /locus_tag="OO249_003038"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5637257.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVVGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(8894..9334)
                     /locus_tag="OO249_003039"
     CDS             complement(8894..9334)
                     /locus_tag="OO249_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5637258.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            9454..11187
                     /gene="ggt"
                     /locus_tag="OO249_003040"
     CDS             9454..11187
                     /gene="ggt"
                     /locus_tag="OO249_003040"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5637259.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAVAAPPVSYGVEEDIFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAQPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(11225..11509)
                     /locus_tag="OO249_003041"
                     /pseudo
     CDS             complement(11225..11509)
                     /locus_tag="OO249_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
     gene            complement(11709..11864)
                     /locus_tag="OO249_003042"
     CDS             complement(11709..11864)
                     /locus_tag="OO249_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637260.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT         2708 a         2955 c         3386 g         2877 t
ORIGIN      
        1 aaaggcactt ttttctgttt atctatcaat aaattcagaa tattatctgt tcttaatcga
       61 ctgaaagata gggattttaa tcactattat cacaaaatac tgcgctaaac ccttaatcag
      121 acaggcaaaa acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataaaaaca
      181 aagcaacaca acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca
      241 tcgcagggat gattgcactg gcaatttcac acaccgctat ggctgacgat attaaagtcg
      301 ccgttgtcgg cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg
      361 cgcgtcaggc gattaaagac attaatgcca aagggggaat taagggcgac aagctggttg
      421 gcgtggaata tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg
      481 ttaatgacgg cattaaatac gttattggtc atctgtgttc ttcttctacc caacctgcat
      541 cagatatcta cgaagacgaa ggtattctga tgatctcacc gggagcgacc aacccggagc
      601 tgacccaacg cggttatcaa cacattatgc gtactgccgg gctggactct tcccaggggc
      661 caacggcggc aaaatacatt cttgagacgg tgaagcccca gcgcatcgcc atcattcacg
      721 acaaacaaca gtatggcgaa gggctggcgc gttcggtgca ggacgggctg aaagcggcta
      781 acgccaacgt cgtcttcttc gacggtatta ccgccgggga gaaagatttc tccgcgctga
      841 tcgcccgcct gaaaaaagaa aacatcgact tcgtttacta cggcggttac tacccggaaa
      901 tggggcagat gctgcgccag gcccgttccg ttggcctgaa aactcagttt atggggccgg
      961 aaggtgtggg taacgcatca ttgtcgaata ttgccggtga tgctgccgaa ggcatgttgg
     1021 tcactatgcc aaaacgctat gaccaggatc cggcaaacca gggcatcgtt gatgcgctga
     1081 aagcagacaa gaaagatccg tccgggcctt atgtctggat cacctacgcg gcggtgcaat
     1141 ctctggcgac tgcccttgag cgtactggca gcgatgagcc gctggcgctg gtgaaagatt
     1201 taaaagctaa cggtgcaaac accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc
     1261 ttaagggatt tgattttggt gtcttccagt ggcacgccga cggttcatcc acggcagcca
     1321 agtgatcatc ccaccgcccg taaaatgcgg gcgggtttag aaaggttacc ttatgtctga
     1381 gcagtttttg tatttcttgc agcagatgtt taacggcgtc acgctgggca gtacctacgc
     1441 gctgatagcc atcggctaca ccatggttta cggcattatc ggcatgatca acttcgccca
     1501 cggcgaggtt tatatgattg gcagctacgt ctcatttatg atcatcgccg cgctgatgat
     1561 gatgggcatt gataccggct ggctgctggt ggcggcggga ttcgtcggcg caatcgtcat
     1621 tgccagcgcc tacggctgga gtatcgaacg ggtagcctat cgcccggtgc gtaactctaa
     1681 gcgcctgatt gcgctcatct cggcaatcgg catgtccatc ttcctgcaaa actacgttag
     1741 cctgactgaa ggttcgcgtg acgtggcgct gcccagtctg tttaacggtc agtgggtggt
     1801 ggggcatagc gaaaacttct ctgcctctat taccaccatg caggcagtga tctggattgt
     1861 tactttcctc gccatgctgg cgctgacgat tttcattcgt tattcccgta tggggcgcgc
     1921 ctgccgtgcc tgcgcggaag atctgaaaat ggcgagcctg cttggcatca acaccgaccg
     1981 ggtgattgct ttgacctttg ttattggcgc ggcgatggcg gcagtggctg gcgtactgct
     2041 cggtcagttc tacggcgtca ttaaccccta tatcggcttt atggccggga tgaaagcctt
     2101 taccgctgcg gtgctcggtg ggattggcag cattccaggg gcgatgattg gtgggctgat
     2161 cctggggatt gcggaggcgc tctcttctgc ctatctgagt acggaatata aggatgtggt
     2221 ttcatttgcc ctgctgattc tggtgctgct ggtgatgccg accggtattc tgggtcgccc
     2281 ggaggtagag aaagtatgaa accgatgcat attgcaatgg cgctgctctc tgccgcgatg
     2341 ttttttgtcc tggcgggcgt ctttatgggc gtgcaactgg agctggatgg caccaaactg
     2401 gtggtcgaca cggcttcaga catccgttgg cagtgggtgt ttatcggcac ggcggtggtc
     2461 tttttcttcc agcttttgcg accggctttc cagaaagggc taaaaagcgt ttccggaccg
     2521 aagtttattc tgcccgcgat tgatggctcc acggtgaagc agaaactgtt cctcgtggcg
     2581 ctgttggtgc ttgcggtggc gtggccgttt atggtgtcac gtgggacggt ggatatcgcc
     2641 actctgacca tgatctacat tatcctcggt ctcgggctga atgtggttgt tggtctttct
     2701 ggtctgctgg tgctggggta cggcggtttt tacgccatcg gcgcttacac ctttgcgctg
     2761 ctcaatcact attacggctt gggcttctgg acttgtctgc cgattgccgg gttaatggca
     2821 gcggcggcgg gcttcctgct cggctttccg gtgctgcgtc tgcgcggtga ttatctggcg
     2881 atcgttaccc tcggcttcgg cgaaattgtg cgcatattgc tgctcaataa caccgaaatt
     2941 accggcggcc cgaacggaat cagtcagatc ccgaaaccga ctttcttcgg ccttgagttc
     3001 agccgtaccg cccgtgaagg cggttgggac acgttcagta atttctttgg cctgaaatac
     3061 gatccctccg atcgcgtcat cttcctgtat ctggtggcgt tgctgctggt ggtgctaagc
     3121 ctgtttgtca ttaaccgcct gctgcggatg ccgctggggc gtgcgtggga agcgttgcgt
     3181 gaagatgaga tcgcctgccg ttcgctgggc ttaagcccgc gtcgtatcaa gctgaccgct
     3241 tttaccatca gtgccgcgtt tgccggtttt gccggaacgc tgtttgctgc gcgccagggc
     3301 tttgtcagcc cggaatcctt cacctttgct gaatcggcgt ttgtactggc gatagtggtg
     3361 ctcggcggta tgggatcgca gtttgcggtg attctggcgg caattttgct ggtggtgtcg
     3421 cgcgagttga tgcgtgattt caacgaatac agcatgttaa tgctcggtgg tttgatggtg
     3481 ctgatgatga tctggcgtcc gcagggcttg ctgcccatga cgcgcccgca actgaagctg
     3541 aaaaacggcg cagcgaaagg agagcaggca tgagtcagcc attattatct gtgaacggcc
     3601 tgatgatgcg cttcggcggc ctgctggcgg tgaacaacgt caatcttgaa ctgtacccgc
     3661 aggagatcgt ctcgttaatc ggtcctaacg gtgccggaaa aaccacggtc tttaactgcc
     3721 tgaccggatt ctacaaaccc actggaggca ccattttact gcgcgatcag catctggaag
     3781 gtttaccggg gcagcaaatt gcccgcatgg gcgtggtgcg taccttccag catgtgcgtc
     3841 tgttccgtga aatgacggtg attgaaaacc tgctggtggc gcagcatcag caactgaaaa
     3901 ccgggctgtt ctctggcctg ttgaaaacgc catccttccg tcgcgcccag agcgaagcgc
     3961 tcgatcgcgc cgcgacctgg cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg
     4021 cgagtaacct ggcctatggt gaccagcgcc gtcttgagat tgcccgctgc atggtgacgc
     4081 agccggagat tttaatgctc gacgaacctg cggcaggtct taacccgaaa gagacgaaag
     4141 agctggatga gctgattgcc gaactgcgca atcatcacaa caccactatc ttgttgattg
     4201 aacacgatat gaagctggtg atgggaattt cggaccgaat ttacgtggtc aatcagggga
     4261 cgccactggc aaacggtacg ccggagcaaa tccgtaataa cccggacgtg atccgtgcct
     4321 atttaggtga ggcataagat ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac
     4381 tacggcaaaa tccaggcact gcatgaggtg agcctgcata tcaatcaggg cgagattgtc
     4441 acgctgattg gcgcgaacgg ggcggggaaa accaccttgc tcggcacgtt atgcggcgat
     4501 ccgcgcgccg ccagcgggcg aattgtgttt gatgataaag acattaccga ctggcagacg
     4561 gcgaaaatca tgcgcgaagc ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg
     4621 atgacggtgg aagagaacct ggcgatgggc ggcttttttg ccgaacgcga ccagttccag
     4681 gagcgcataa agtgggtgta tgaactgttt ccacgtctgc atgagcgccg tgttcagcgg
     4741 gcaggcacca tgtccggcgg tgagcaacaa atgctggcga ttggtcgcgc gctgatgagc
     4801 aacccgcgtt tgctgctact tgatgagcca tcgctcggtc ttgcgccgat tatcatccag
     4861 caaattttcg acaccatcga gcagctgcgc gagcagggga tgactatctt cctcgtcgag
     4921 cagaacgcca accaggcgct aaagctggcg gatcgcggct acgtgctgga aaacggccat
     4981 gtggtgctct ccgataccgg cgatgcgctg ctggcgaacg aagcagtgag aagtgcgtat
     5041 ttaggcgggt aataacacgt tgattgataa ggagtcaaaa gactcctttg agacaggtga
     5101 caaatgtaaa attgcctgat gcgctgcgct tatcaggcct actgggtgag ttgcaatata
     5161 ttgaatttgc acgatcttgt aggccggata aggcgtttac gccgcatccg gcatgaaacg
     5221 atgagtaatc tgtagagttt gattcagacc ttctattttt ccgcttatcc gtgccccatc
     5281 tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac cttactatct
     5341 tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt accccgcgag
     5401 cataaaacgc gtgaattcgc gcattcggta caacaagaga gataaacgat gaaaccgtta
     5461 cattatacag cttcagcact ggcgctcgga ctggcgttaa tggggaatgc acaggcagtg
     5521 acgaccattc cgttctggca ttctatggaa ggggaactgg gtaaagaggt ggattctctg
     5581 gcccaacgtt ttaacgccga aaatccggat tacaaaattg taccgaccta taaaggcaac
     5641 tacgaacaga atttaagcgc ggggattgcc gcatttcgta ccggcaacgc tccggctatt
     5701 ttgcaggttt atgaagttgg caccgccacc atgatggcat cgaaagccat taaaccggta
     5761 tatgacgtgt ttaaagaggc ggggattcaa ttcgatgagt cgcagtttgt gccgacggtt
     5821 tccggttact actccgacag caaaacgggc cacttactct cccagccatt caacagctcg
     5881 actcccgttc tctattacaa caaagacgcc ttcaagaaag cggggttaga cccggaacag
     5941 ccgccgaaaa cctggcagga tctggcggac tatgccgcga aactgaaagc ctccggcatg
     6001 aagtgcggct acgccagcgg ctggcagggc tggatccaac tggaaaatcg acaccttccc
     6061 ggtgatcgac gccgccacgt ccggcgggcc ggttcaggcc accacgacgc tgatttataa
     6121 gatctaccgc gaaggtttta ccggactgga tctggcttcg tctgccgcac agtcggtggt
     6181 gttgatgttc ctcgtcatcg tgctgacggt ggtgcagttc cgctatgttg aaagcaaggt
     6241 gcgttaccaa tgattgagaa ccgtccgtgg ctgacgatat tcagccatac catgctgatc
     6301 ctcgggatcg cggtgattct cttcccgctg tacgtggcgt ttgtcgcggc gacgctggat
     6361 aaacaggccg tctatgccgc gccgatgacg ctcatccccg gcacacatct gctggaaaat
     6421 atccacaaca tctgggttaa cggggtaggt acgaatagcg cgccgttctg gcggatgttg
     6481 cttaacagct ttgtgatggc gttcagcatt acgctcggca aaattaccgt ctcgatgctc
     6541 tcggcatttg ccattgtctg gtttcgtttt ccgctacgta acctcttctt ctggatgatt
     6601 tttatcaccc tgatgctgcc ggttgaagta cgtatcttcc cgacggtgga agtcatcgcc
     6661 aacctgaaga tgctcgacag ctacgccggt ttaacgctgc cgctgatggc ctcggcgacc
     6721 accacgtttc tgtttcgcca gttctttatg acgctgccgg atgaactggt ggaagcggcg
     6781 cggatcgacg gcgcatcgcc aatgcgcttc ttttgcgaca tcgtttttcc gctctccaaa
     6841 actaatctgg cggcgctgtt tgtgatcacc tttatttacg gctggaacca gtatttgtgg
     6901 ccgttgttga ttattaccga cgtggatctc ggcaccaccg tggcagggat caaagggatg
     6961 atcgccactg gcgaaggcac cacggaatgg aactcagtga tggcggcgat gttgttaacc
     7021 ctgatcccac cagtagtgat tgttttagtg atgcagcgtg ccttcgtgcg cggcctggtc
     7081 gatagtgaga aataagatgg caggactgaa attacaggca gtaaccaaaa gctgggatgg
     7141 caaaacccag gtcattaaac cgctgaccct tgatgtggct gatggcgaat ttatcgtgat
     7201 ggtcgggccg tctggctgcg ggaaatcgac gctgctgcgt atggttgccg ggctggagcg
     7261 ggtgaccgaa ggcgatatct ggatcaacga ccagcgcgtg actgaaatgg agccgaaaga
     7321 tcgcgggatt gcgatggtgt tccagaacta cgcgctttat ccgcatatga gcgtcgaaga
     7381 aaacatggcg tgggggctta aaattcgcgg catgggcaag cagcaaattg ccgagcgcgt
     7441 taaagaagcg gcgcgcattc tggagctgga cggtctgctc aaacgtcgcc cgcgtgagct
     7501 atccggcggt cagcgtcagc gtgtagcgat gggccgcgcg attgtgcgcg atccggcggt
     7561 gttcctgttt gatgagccgc tctctaacct cgatgccaag ctgcgcgtgc agatgcgtct
     7621 tgaactgcaa cagttgcacc gtcgcctgaa aacgacttca ctctacgtta ctcacgatca
     7681 ggttgaagcg atgacgctcg cccagcgagt aatggtgatg aacggcggcg ttgccgaaca
     7741 gattggcaca ccagttgaag tctacgaaaa gcccgccagc ctgtttgtag cgagttttat
     7801 cggcagtccg gcgatgaacc tgctgacagg ccgcgtgaat aacgaaggca cgcatttcga
     7861 actggacggc ggtattgagc tgccgctaaa cggtggctac cgtcagtatg ccgggcgtaa
     7921 aatgactctc ggcattcgcc cggaacatat tgtgctaagc tcgcaggcag aaggcggcgt
     7981 acctctggtg atggacacgc tggagatcct cggcgcagat aacctggcgc acggacgctg
     8041 gggcgagcag aagctggtgg tgcgactggc gcatcaggag cgcccgacgg caggcagcac
     8101 gctgtggctg catctggcgg aaaatcagct gcatcttttt gatggtgaaa caggacaacg
     8161 agtatgagta actggcctta tccccgcatc gtcgctcatc gtggcggcgg taagctggcc
     8221 ccggaaaaca ccctggcggc aatcgacgtc ggggcaaaat acggtcataa gatgatcgaa
     8281 tttgacgcga agttatcgaa agatggcgag atcttcctgc tccatgacga caatctcgaa
     8341 cgcaccagca acggctgggg cgtcgtgggt gaactgaact ggcaggattt actgcgcgtg
     8401 gatgcgggca gttggtacag caaagcgttt aaaggtgagc cgctgccgtt gctttcgcag
     8461 gtggcggaac gctgtcgcga acatgggatg atggcgaata tcgaaatcaa acccaccact
     8521 ggcactggac cattaacggg caaaatggtg gcgctggcgg cacgccaact gtgggccggt
     8581 atgacgccgc cgctgctgtc atcgtttgag attgatgctt tagaagctgc acagctggcg
     8641 gcaccggaac tgccgcgtgg tttattgctt gatgagtggc gcgacgactg gcgcgaactg
     8701 accgcgcggc tgggctgcgt ctctattcat ctcaatcata agttactcga taaagcgcga
     8761 gtgatgcagt tgaaagacgc cggattacgg attctggttt ataccgtcaa taaaccccag
     8821 cgcgcggcag agttgctgcg ctggggcgtg gattgcatct gtaccgatgc gattgacgtg
     8881 attggtccga actttacggc ccaatagttt tcaacggaat gtcaggctgc ggcgtgctgg
     8941 gctgattcag catgtcgccg tttctcctct ccggcagcat atgctgctga ttcaacgaac
     9001 tatccggatt acggttgctg tttaacatcc cgccgttggt gttgggcagc atttgctgcc
     9061 gggcgggatt tcgctccccc ggctgtgact gcaacacccg ctgagaatta ttgtttatct
     9121 ggttttctaa atgctgctgt tgcaactgag tttgcgtttt cagttgctga ttcagcatcc
     9181 ctttttgctg gatttgctga gtttgcatct gggtttgcat ccgctgctgg ctgggtatct
     9241 gataccccgg ctggttaggg ttgttcagag tattaatggg ctgtgcaaag ccgacaaacg
     9301 gcaggagtgc cgtaagaatc agaagtcgtt tcatcgcgta tcctcctctg aagatatcct
     9361 ttaagtttac tcgcttcccg acaaaacgat gattaattca gagttatata ccaggcttag
     9421 ctggggttgc cccttaatct ctggagaata acgatgataa aaccgacgtt tttacgccgg
     9481 gtagccattg ctgctctgct ctcaggaagt tgttttagcg ccgtcgccgc gcctcctgtt
     9541 tcttacggcg tggaggaaga tatcttccat ccggtacgcg cgaaacaggg gatggtggcg
     9601 tctgtcgatg ccactgccac acaagtgggg gtagatattc taaaggaggg cgggaatgcc
     9661 gttgatgccg ccgtggcggt gggctacgcg ctggcggtaa cgcatccgca ggcagggaat
     9721 ctgggcggtg gtggttttat gctgatccgc tcgaaaaatg gcaataccac cgctatcgat
     9781 ttccgcgaga tggcacccgc taaagcgacc cgcgatatgt tccttgatga ccagggcaac
     9841 ccggacagca aaaaatcact cacttcgcat ctggcttccg gcacgcccgg tacggtcgct
     9901 ggtttctcgc tggcgttgga taagtacggc actatgccgt tgaacaaagt ggtgcagccc
     9961 gcgttcaaac tggcacgcga tggttttatc gttaacgatg ctttggcaga cgatctcaaa
    10021 acctacggta gcgaagtgtt gccgaatcac gaaaacagca aagctatctt ctggaaagag
    10081 ggcgaaccgc tgaaaaaggg cgacaagctg gtgcaggcga acctggcaaa gagcctggag
    10141 atgattgccg aaaacggccc ggacgaattc tataaaggca cgatagcgga acagattgcc
    10201 caggagatgc agaaaaacgg tggcttgatc actaaagaag atttagcggc gtataaagcg
    10261 gtcgaacgca caccgataag tggcgattat cgcgggtatc aggtttactc catgccaccg
    10321 ccatcctccg gcgggatcca tatcgtacaa atcctcaata ttctggaaaa cttcgatatg
    10381 aagaaatacg gctttggcag cgccgatgcg atgcaaatca tggcagaagc ggagaaatac
    10441 gcctacgccg accgctcgga atatcttggc gacccggatt ttgtcaaagt accgtggcag
    10501 gcgctgacca ataaagccta tgccaaatct attgccgatc aaattgatat caataaagcg
    10561 cagccatcca gcgaaattcg ccccggcaag cttgcgcctt atgagagtaa tcaaactacc
    10621 cattactcag tggtggataa agatggtaac gcggtggcgg tgacctatac gctgaacacc
    10681 accttcggta cgggcattgt cgcgggcgag agcggtattc tgcttaataa tcagatggat
    10741 gatttctccg ctaaaccggg cgtaccgaac gtttacgggc tggtgggcgg tgatgccaac
    10801 gccgtcgggc cgaataaacg tccgctgtcg tcaatgtcac caacgattgt ggtgaaagac
    10861 ggtaaaacct ggctggtcac cggtagccct ggcgggagcc ggatcatcac tacagtgctg
    10921 caaatggtgg tgaatagcat cgattatggc atgaacgtcg ccgaagcgac caatgcgccg
    10981 cgtttccacc atcagtggtt gccggacgag ctgcgtgtcg aaaaagggtt tagcccggat
    11041 acgctcaagc tgctggaagc aaaaggtcag aaagtggcgc tgaaagaggc gatgggcagt
    11101 acacaaagca ttatggttgg gccggacggt gagttgtacg gcgcatccga cccgcgctcg
    11161 gtggatgatt taacggcggg gtactaaggt tagcggccct cttcgtggga agagggctat
    11221 tttgtcaggg caagccgaag gtagcctttt ttatttcgta atcctgtaga tattcttcca
    11281 gcggctttgc cgttcccaga gaatgaattt caccactgtc tttatcaata ataaaaggtg
    11341 cgttaccagc taagcgcgcg gcctcatctc cagtttcgag aaattctcgt gcttcgaaac
    11401 agaaatacca gccctagcta aagcgtccat gtagagtaat gacgaccggg agatctgcat
    11461 catcaaggta atggttcgct ttcgcgaatg cgtcgtgata agtaatcata attataataa
    11521 atattattgt tgagtgttat attattattc ctgtgtgata cattgagcaa agacgcgttc
    11581 atcttcgtca aagatattat taaatgctgg ttttgaaaaa agttcatcgg ataagatata
    11641 gtcacgaaat aagtatctgc taatatttta tgatttcttt ttcagaacgt cgaacgggat
    11701 tttcttattt aagatatcag aatcttatga ttcgggtaaa attctaaata aatcaacatg
    11761 tcattaaaat catcatagcc attagctatt tcggctaaaa tagagactac atgtcttcgg
    11821 tccatctcac ttaaggagtg tagttccgtt gtaagttttt ccatagcttg cactgctaaa
    11881 tttcgaacaa ggaattttct gctggtaatc tctaaaaaga tggcat
//
LOCUS       DAJTOR010000128        11869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTOR010000128 DAJTOR010000000
VERSION     DAJTOR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.128"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(32..346)
                     /locus_tag="OO249_003043"
                     /pseudo
     CDS             complement(32..346)
                     /locus_tag="OO249_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            complement(461..859)
                     /locus_tag="OO249_003044"
     CDS             complement(461..859)
                     /locus_tag="OO249_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HCR5637261.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQLESAPGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1099..3513)
                     /gene="gyrB"
                     /locus_tag="OO249_003045"
     CDS             complement(1099..3513)
                     /gene="gyrB"
                     /locus_tag="OO249_003045"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5637262.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3542..4615)
                     /gene="recF"
                     /locus_tag="OO249_003046"
     CDS             complement(3542..4615)
                     /gene="recF"
                     /locus_tag="OO249_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5637263.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLVEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4615..5715)
                     /gene="dnaN"
                     /locus_tag="OO249_003047"
     CDS             complement(4615..5715)
                     /gene="dnaN"
                     /locus_tag="OO249_003047"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5637264.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5720..7123)
                     /gene="dnaA"
                     /locus_tag="OO249_003048"
     CDS             complement(5720..7123)
                     /gene="dnaA"
                     /locus_tag="OO249_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5637265.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7420..7500
                     /locus_tag="OO249_003049"
     CDS             7420..7500
                     /locus_tag="OO249_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637266.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7730..7870
                     /gene="rpmH"
                     /locus_tag="OO249_003050"
     CDS             7730..7870
                     /gene="rpmH"
                     /locus_tag="OO249_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5637267.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            7887..8246
                     /gene="rnpA"
                     /locus_tag="OO249_003051"
     CDS             7887..8246
                     /gene="rnpA"
                     /locus_tag="OO249_003051"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5637268.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8210..8467
                     /gene="yidD"
                     /locus_tag="OO249_003052"
     CDS             8210..8467
                     /gene="yidD"
                     /locus_tag="OO249_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5637269.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8470..10116
                     /gene="yidC"
                     /locus_tag="OO249_003053"
     CDS             8470..10116
                     /gene="yidC"
                     /locus_tag="OO249_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5637270.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10222..11586
                     /gene="mnmE"
                     /locus_tag="OO249_003054"
     CDS             10222..11586
                     /gene="mnmE"
                     /locus_tag="OO249_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5637271.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            11829..>11869
                     /locus_tag="OO249_003055"
     CDS             11829..>11869
                     /locus_tag="OO249_003055"
                     /inference="COORDINATES: protein motif:HMM:NF019665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5637272.1"
                     /translation="MNILHICVTSKWF"
BASE COUNT         2713 a         3250 c         3064 g         2842 t
ORIGIN      
        1 ggtatcgctt agtttttcaa tttcctatac gtgaaccgac ttcgcgagag agtttttcca
       61 ggaaacgata gtcgtcataa ctgagagcag tttgcgccac ggtgctacca tcagcggcct
      121 tctgtaccag cgcgccgtta taagtaatgc agtagtcgcc cggctgttcc atatgcagct
      181 ctttcaggta gttgtgaacg cctgcataag ggcgacccgt cgttagcacg acattcacgc
      241 cacgggcgcg agctgcggca atcgcatttt taacggcggg tgaaatggtg tgatcgggca
      301 gcagaagggt gccatccata tcgatagcaa tgagtttgat agccatgagt tccccaggta
      361 gattagttcc tgacccatgc taacgcgatt ccgctcaaaa atcagtacaa cacccgaggg
      421 aaaaggggga tgcaacgcgg gttgcatgct ccccatttgc ttagcggaag agtttccctt
      481 tcagcagttc catacctgcg gaaagcagat cgttattggc ttgtgaagac acttcacctt
      541 gcggtgagag cgcatcaata atcttcggca attgttctgc cagtaaactg gaagccgtac
      601 tggtatccac gccaagtttt tgcccgagat cggacaccgc atttgtgccg ggcgccgatt
      661 ccagttgctc gccgctaact gattgattgc tctgctgatt actcaaccag gttgagagaa
      721 tggcccctaa gccgccactt tgcagttttt ccagcagcac ctgaatgccg ccctgctcct
      781 caacccaact taaaatagcc tgatatttcc ccgcatcgcc tttcagaaag gcaccgacaa
      841 cttcatcaaa aagccccatg ataatcacct gtaaagcgtt acgtgttgac ccaaaaagta
      901 tagatctgcg ggatgataat tgcggattgc agaaataaaa agggcggaga tgatctccgc
      961 ccttttttta tagcttcttg ccggatgcgg cgttaacgtc ttacctggcc tacaaaatca
     1021 ttaaaattca atacattgca agattttcgt aggcctgata agcgtagcgc atcaggcacg
     1081 ctcgcatggt taacgccatt aaatatcaat attcgccgct ttcagggcgt tctcttcaat
     1141 aaacgcacga cgcggttcta cggcgtcgcc catcagcgtg gtgaacaaca ggtcagcggc
     1201 aatcgcatcc ttaacggtaa cgcgcagcat acgacgactt tccgggtcca tggtggtttc
     1261 ccacagctgt tccgggttca tctcgcccag acctttataa cgctggatgg agagaccgcg
     1321 acgagactct ttcaccagcc agtccagcgc ctgctcgaag ctggctaccg gctgacgacg
     1381 ctcgccacgt tcgataaatg catcttcttc cagcaagcca cgcagtttct cacccagcgt
     1441 gcagatacga cgatattcgc caccagtgat aaattcgtga tccagcggat agtcagtatc
     1501 cacaccgtgg gtacgcacgc gaacaatcgg ctcgaacagg ttttgttcgg cattggtgtg
     1561 gacatcaaac ttccactggc tgccgtgctg ttctttgtcg ttcagttcgc tgaccagcgc
     1621 gttcacccag cgggtaacgg tctgctcatc agagaggtcg gcttccgtca gcgtcggctg
     1681 atagataagc tctttcagca ttgctttcgg ataacgacgc tccatgcggt tgatcatttt
     1741 ctgcgtcgcg ttgtactcag acaccagttt ctctaacgct tcgccagcca atgccggtgc
     1801 gctggcgttg gtgtgcagcg ttgcgccgtc cagcgcgata gagatctggt actgatccat
     1861 cgcttcgtcg tctttaatgt actgttcctg cttgcctttc ttcactttgt acagcggcgg
     1921 ctgagcgatg tagacgtgac cgcgttcaac gatttccggc atctgacgat agaagaaggt
     1981 caacagcagc gtacgaatgt gcgagccgtc gacgtccgca tcggtcatga tgatgatgct
     2041 gtgataacgc agtttgtccg ggttgtactc gtcacgaccg ataccacagc caagcgcggt
     2101 gataagcgtc gccacttcct gagaagagag catcttatcg aagcgcgctt tctcaacgtt
     2161 gaggatttta cccttcagcg gcagaatcgc ctggttcttg cggttacgcc cctgcttcgc
     2221 agagccgccc gcggagtccc cttccaccag gtacagttcg gaaagcgccg gatcgcgttc
     2281 ctggcagtct gccagtttgc ccggcaggcc cgctaagtcg agcgcacctt tacggcgggt
     2341 catttcacgc gcgcgacgcg ccgcttcacg ggcacgggca gcatcgataa ttttgccaac
     2401 cacgattttc gcgtcggttg ggttttccag caggtattct gccagcagtt cgttcatctg
     2461 ctgttcaacc gccgatttca cctcagaaga aaccagtttg tctttggtct gggaggagaa
     2521 tttcgggtcc ggcactttta cggaaacgac cgcaatcagg ccttcacgcg catcgtcacc
     2581 ggtggcgctg actttggctt ttttgctgta gccttctttg tccatgtagg cgttcagggt
     2641 acgggtcatc gccgcacgga agcctgccag gtgagtaccg ccgtcacgct gcggaatgtt
     2701 gttggtaaag cagtagatgt tttcctggaa gccatcgttc cactgcaacg ccacttcgac
     2761 gccaataccg tctttttcgg tggagaagta gaagatattc ggatggatcg gcgttttgtt
     2821 cttgttcaga tattcaacga acgccttgat gccgccttca tagtggaagt ggtcttcttt
     2881 gccgtcacgc ttgtcgcgca gacgaatgga aacgccggag ttgaggaacg acaactcacg
     2941 cagacgtttc gccagaattt catattcgaa ctcggtcaca ttggtgaagg tttcgaggct
     3001 gggccagaaa cgcaccatgg tgccggtttt ttcagtctcg ccggtaaccg ccagcggagc
     3061 ctgcggtaca ccgtgttcgt agatctgacg gtgaatttta ccctcgcgct ggataaccag
     3121 ctccagtttt tgcgacaggg cgtttactac cgaaacacca acgccgtgca gaccgccgga
     3181 cactttatag gagttatcgt caaatttacc gcctgcgtgc agaacggtca tgatcacttc
     3241 cgccgccgat acgccctctt ccgggtgaat accggtcgga atgccgcgcc cgtcatcctg
     3301 tacagagaca gagttatcgg cgtgaatggt gacgataatt tctttacagt gacccgcgag
     3361 cgcttcgtcg atagcgttat ctaccacctc gaataccatg tggtgcagac cggtgccgtc
     3421 atccgtgtcg ccgatataca tacccgggcg cttacgcacc gcatccagcc ctttcaggac
     3481 tttgatactg gaggagtcat aagaattcga catcaacgtt tctcgctcat ttatacttgg
     3541 gttaatccgt tattttaccc ttttccacgg taaacatctt cgaattttcg tccgacatgt
     3601 ctataacgtg ttcagcactg atcgcgctga caaagacctg tgattgcgtc gcttttaagc
     3661 ggctggcaag cagcccgcga cgctcatcat caagctcaga ggcaaaatca tctatcaggt
     3721 agagacaccg ccgcccgctt tcacgggtga ggaactctcc ttgcgccaga cgtaaggcgc
     3781 acatcaacag cttaagctgc ccacgcgata aggtatcttc caccggcgca ccgtcggcgc
     3841 gaatgcgtaa gtccgcttta tgcgggccat gcgcggtgta ggttagctgg cgatcgcgtt
     3901 caaaattacg ttccagcacc tcagcatatt ctgtctcttt ctcccagccg cgctggaaag
     3961 agaaagtcag agaaaactca gggagaaatt gcttacaagt atcggccata tcagccgcga
     4021 taccggcgct atactccgcg cgccaggtgc tgatttgctc caccagcggg atcagctctt
     4081 tatcccacgg gcgtagctgt tcgtaacgtg tcacctggcg cagcgccgca ttgcgctgct
     4141 tgagcaatcg cttgagattg ctccaggcgg tgaaaaatcc gggttcgttg tgaaagcatg
     4201 cccagtcgag aaatgctctt ctgtatttgg ggccgccgtt gagtaaagta aacccttctg
     4261 gcgttatcag ctgcatcggc atcaggtgcg ccagctccgc gactttatgc ccgtcagtac
     4321 cgtcgatgcg gactttgctg tcgccttgtt tgtctttggt taagccaatc gccgtctcgc
     4381 gctcttcgcc ctgtaatcgc ccatggagaa caaatgcctc ctgctcatgg cgaatgacgc
     4441 gaccaatctg caaactgcga aacgcccgac catggccgag cgtatagatg gcttccagca
     4501 cactggtttt gccactgccg ttggcaccta ccagaaagtt aaagccggga gataaggcga
     4561 gatccgcggt ttcgatattg cggaaatcgc ggatcaacaa gcgggtgagg gacattacag
     4621 tctcattggc atgacaacat aagccgcgct ctggctggcc gcatcttcaa tctgcacgct
     4681 ggaaaccgaa tcggtcagca tcatgcggac gttttcgcat ttcagcgcgt tcagaacatc
     4741 cagcacatag ctgacgttga agccgatttc catctccgca ccgctatagg taacgtcgag
     4801 gatctcttcc gcttcttcct gttccgggtt gttggcagtg attttcagct ggttttcgct
     4861 gacataaaga cgtacgccgc ggaatttctc gttagagaga attgccgcac gggcaaacgc
     4921 ctgcttgagc agatcgcagc cagcttccag atgtttgtcc gggttcttcg gtagaacgcg
     4981 gcggtaatcc gggaagcgac catccaccag tttggaggtg aagataaagt cgccaacgtg
     5041 ggcacgaatg ttattgctgc caatctgcac gcgcagcgga ttgtcgccgc cgtcgagcat
     5101 acgcatcagt tcaatcacgc ctttacgcgg cacgatcacc gaatggcttg gcaaagattg
     5161 accaattggc attgaacaga ctgccagacg gtgaccatcg gtcgctacgg tgcgcagttc
     5221 ttcgccttcg gtttcaaaca gcataccgtt taagtaatag cgaacatcct gatgcgccat
     5281 cgaaaactgg gtcgcttcaa tcagacgctt catcgtcgcc tgcggcaggg taaattcgac
     5341 ttcgctctgc cagtcatcga ggttcgggaa atccgccgct ggcagggtag acagcgaaaa
     5401 acggctacgc ccggagcgca ccagcatccg ttcaccttcc agctgcacgg caatttccgc
     5461 gccttcaggc agaccacggc agatatcaaa gaatttgcgc gccggaacgg tcgttgctcc
     5521 tggctcgtgt ggctgaacca gcgcaacacg tgccaccatt tccatctcga gatcagtacc
     5581 ggtcagcgac aacgtaccgt cagcaacctg taacagcaga ttaccgagaa tcggtagcgt
     5641 aggacgacca cctaacggac cgctcacctg ttgtagcggt tttaataaat gctcacgttc
     5701 tacggtaaat ttcataggtt tacgatgaca atgttctgat taaatttgaa aaatcttctt
     5761 tgatatcgtg gctctcttca cgcaactgct cgatcttacg gcaggcatga agcaccgtcg
     5821 tgtggtcacg accaccaaac gcatcgccaa tctccggcag actgtggtta gtcagctctt
     5881 tcgccagcgc catcgccatc tggcgcggac gcgccaccga gcgggatcga cgcttggaaa
     5941 ggagatccgc gactttgatc ttgtagtact ccgccaccgt cttctgaata ttgtcgatgg
     6001 tgaccagttt ttcctgcaat gccagcaagt cgcgcagcgc ctcacgcacg aagtcgatgg
     6061 tgatcgcccg tccggtaaag ttggcattgg caatgacgcg gttcagcgcc ccttccagct
     6121 cacgtacgtt agatcgtaga cgcttggcga taaagaacgc cacttcgccc ggcaaacgaa
     6181 tgtcgttttc gtcggccttt ttcatcagga tcgccacacg ggtttccagc tctggcggtt
     6241 cgatcgccac agtcagtccc caaccgaagc gggatttcaa acgatcctca acgccgttga
     6301 tctctttcgg atagcgatcc gaggtgagaa tgatctgttg attaccttcc agcagggcgt
     6361 tgaaggtatg gaaaaactct tcctgagatc gttctttatt agcaaaaaac tgaatatcgt
     6421 cgatcagcag tgcatctacg gaacggtagt agcgtttaaa ctcttcgatc gcgttgtttt
     6481 gcagggcttt aaccatgtcc tgaacaaagc gctcggagtg catataaacc actttggcat
     6541 tcggcttgcg cgccataatg ccgttaccca ccgcatgcag taggtgagtt ttacccagac
     6601 ccgtgccgcc ataaaggaac aacgggttat aggcaccgcc agggttatcc gccacctggc
     6661 gagccgccgc gcgcgccagt tggttagatt taccttcaac gaagttatca aacgtgtgtt
     6721 tgacgtttac gttagaacga taggtcggtt ctgccggggc cgggacgtta tcccaacctg
     6781 agcgcgtaga aggcgcagca cgttgcggct gcgtttgcgc cacctgtgca ggggccgcga
     6841 cgttgctcgt cactgccgct tgtggcgttt gcgtcaccgg tttggtgccg acttcaaaac
     6901 gcagctgtgg ggcatccgct ccgcagaaac tggttagcag tccattgata ttattaaggt
     6961 acttgtcccg tacccaatcg aggacaaaac ggtttggcgc gtacagggcc agcgtgttat
     7021 cgctcagttc cgcctgcaat gggcgtatcc acatactgaa ttctgtggct ggtaactcat
     7081 cctgcaatcg ggcaagacac tgctgccaaa gcgaaagtga cacggcggac tccactcgaa
     7141 caaaagtcga taatgactaa ggctgaaaca ttcatgattg ttgacgtacg tcgaaaagac
     7201 cctgcttgag ggtgacgcac gaaccgctgt ctgcggttat atgcccgatc aagatcctgc
     7261 aaaacgatcg ggaccgcgga tcatagccta aactgcgcaa gagatcttct gtttctcaca
     7321 gattttcccg atttatccac aggactttcc agaactcgct aagtgtaaac gatcctgccg
     7381 cgaggcgggc acgatttacg ccacatattg aaaaatttaa tgaccataga caaaaattgg
     7441 cttaatcgat ctaataaaga tccaggacga tccttgcgct ttacccatca gcccgtataa
     7501 tcctccaccc ggcgcgccat gctggtttcc actggtgtga ggtcgtacat tttccctgcg
     7561 aaaaggtgcg gaaaagcgcg gtaaataagg aaagagaatt gactccggag tgtacaatta
     7621 ttacaatccg gcctctttaa tcacccatgg cttcggtgtc catcgtttca tttttcggcg
     7681 gatatccaat aaagccattg aatttattca agtttaggta gaaatcgcca tgaaacgcac
     7741 ttttcaaccg tctgtactga agcgcaaccg ttctcacggc ttccgtgctc gtatggctac
     7801 taaaaatggt cgtcaggttc tggcacgtcg tcgtgctaaa ggccgcgctc gtctgaccgt
     7861 ttctaagtaa tcaagctaac ccctgagtgg ttaagctcgc atttcccagg gagttacgct
     7921 tgttaactcc cagtcaattc acattcgtct tccagcagcc acaacgggct ggcacgccgc
     7981 aaattaccat tctcggccgc ctgaattcgc tggggcatcc ccgtatcggt cttacagtcg
     8041 ccaagaaaaa cgttcgacgc gcccatgaac gcaatcggat taaacgtctg acgcgtgaaa
     8101 gcttccgtct gcgccaacat gaactcccgg ctatggattt cgtggtggtg gcgaaaaaag
     8161 gggttgccga cctcgataac cgtgctctct cggaagcgtt ggaaaaatta tggcgccgcc
     8221 actgtcgcct ggctcgcggg tcctgatagc cctcattcgg gtctatcaac gcctgattag
     8281 tccgctactc gggccgcatt gtcgtttcac tccaacctgt tcaagctacg gaattgaggc
     8341 attgcgcagg tttggagtga taaaaggcag ttggttgacg gtgaaacgcg tattaaaatg
     8401 ccacccttta caccctggtg gtgacgatcc cgtcccgccc ggaccatttg ataccagaga
     8461 acactaacga tggattcgca acgcaatctt ttagtcatcg ctttgctgtt cgtgtctttc
     8521 atgatctggc aagcctggga gcaggataaa aacccgcaac ctcaggccca acagaccacg
     8581 cagacaacga ccaccgcagc gggtagcgcc gccgaccagg gcgtaccggc cagtggccag
     8641 gggaaactga tctcggttaa gaccgacgtg cttgatctga ccatcaacac ccgtggtggt
     8701 gatgttgagc aagctctgct gcctgcttac ccgaaagagc tgaactctac ccagccgttc
     8761 cagctgctgg aaacttcacc gcagtttatt tatcaggcac agagcggtct gaccggtcgt
     8821 gatggcccgg ataacccggc taacggcccg cgtccgctgt ataacgttga aaaagacgct
     8881 tatgtgctgg ctgaaggtca aaacgaactg caggtgccga tgacgtatac cgacgcggca
     8941 ggcaacacgt ttaccaaaac gtttgtcctg aaacgtggtg attacgctgt caacgtcaac
     9001 tacaacgtgc agaacgctgg cgagaaaccg ctggaaatct ccacctttgg tcagttgaag
     9061 caatccatca ctctgccacc gcatctcgat accggaagca gcaacttcgc actgcacacc
     9121 ttccgcggcg cggcgtactc cacgcctgac gagaagtacg agaaatacaa gttcgatacc
     9181 attgccgata acgaaaacct gaacatctct tcgaaaggtg gttgggtggc aatgctgcaa
     9241 cagtatttcg cgacggcgtg gatcccgcat aacgacggta ccaacaactt ctataccgct
     9301 aatctgggta acggcatcgc cgctatcggc tataaatctc agccggtact ggttcagcct
     9361 ggtcagactg gcgcgatgaa cagcaccctg tgggttggcc cggaaatcca ggacaaaatg
     9421 gcagctgttg ctccgcacct ggatctgacc gttgattacg gttggttgtg gttcatctct
     9481 cagccgctgt tcaaactgct gaaatggatc catagctttg tgggtaactg gggcttctcc
     9541 attatcatca tcacctttat cgttcgtggc atcatgtacc cgctgaccaa aacgcagtac
     9601 acctccatgg cgaagatgcg tatgctgcag ccgaagattc aggcaatgcg tgagcgtctg
     9661 ggcgatgaca aacagcgtat cagccaggaa atgatggcgc tgtacaaagc tgagaaggtt
     9721 aacccgctgg gcggctgctt cccgctgctg atccagatgc caatcttcct ggcgttgtac
     9781 tacatgctga tgggttccgt tgaactgcgt caggcaccgt ttgcactgtg gatccacgac
     9841 ctgtcggcac aggacccgta ctacatcctg ccgatcctga tgggcgtaac gatgttcttc
     9901 attcagaaga tgtcgccgac cacagtgacc gacccgatgc agcagaagat catgaccttt
     9961 atgccggtca tcttcaccgt gttcttcctg tggttcccgt caggtctggt gctgtactat
    10021 atcgtcagca acctggtaac cattattcag cagcagctga tttaccgtgg tctggaaaaa
    10081 cgtggcctgc atagccgcga gaagaaaaaa tcctgattcg gtgagttttc gctaaaataa
    10141 gggcggtcag ttgaccgcct tttttctttt cgtagggcgg ataagcaccg cgcatccgcc
    10201 acacaaagca acaggaacat catgagcgat aatgacacta tcgtagccca ggccacgcct
    10261 ccgggacgtg gcggcgttgg catcctgcgc atctccggct tcaaagcccg tgaagttgcc
    10321 gaaaccgtgc tgggtaaact gcctaagccg cgctacgccg attatcttcc gtttaaagac
    10381 gccgacggca gcgtgctcga tcaggggatt gcgctatggt tccctggccc gaactcgttc
    10441 accggcgaag atgtgctgga actgcaaggt catggcggtc cggtgatcct cgacctgctg
    10501 ttaaaacgta ttctgaccat tcccggcctg cggattgctc gccctggtga gttttccgaa
    10561 cgcgcgtttc ttaacgataa acttgactta gcccaggccg aggcgattgc cgatcttatc
    10621 gacgccagtt cggaacaggc ggcccgttcg gcacttaact cgctgcaagg cgcattctcc
    10681 gcacgggtta atcatctggt agaagccctc acccacttgc gcatttacgt cgaagcggca
    10741 attgatttcc ccgatgaaga gatcgatttc ctctccgacg gaaaaattga agcccagctc
    10801 aatgacgtta ttgccgatct tgatgcagtg cgtgctgaag cacgtcaggg tagtttgttg
    10861 cgcgaaggga tgaaagtggt gattgccgga cgtcctaacg ccggtaaatc gagcctgtta
    10921 aacgcgctgg cggggcgtga agcggcaatc gtaaccgata tcgccggaac tacgcgtgac
    10981 gtgctgcgtg agcatatcca cattgacgga atgccgctgc atatcatcga taccgccggg
    11041 ctacgtgaag ccagtgacga agtagaacgt attggtatcg agcgcgcgtg gcaggaaatt
    11101 gaacaggccg accgcgtgct gtttatggtc gatggcacca caacagacgc cgtggatccg
    11161 gcagagatct ggccggaatt tattgcccgt ctgccagcga aactgccgat caccgtggtg
    11221 cgcaataaag ccgatatcac cggcgaaacg ctgggaatga gtgaagtgaa cggtcacgcg
    11281 ttaattcgtc tctcggcaag gactggtgaa ggcgtggacg tgctgcgtaa ccatctcaaa
    11341 cagagcatgg gctttgacac caacatggaa ggcggcttcc tggcgcgtcg tcgccaccta
    11401 caggcgctgg aacaggcagc ggaacatcta caacagggca aagcgcaact gttgggagcc
    11461 tgggcaggtg aactgctggc ggaagagttg cgtctggcac agcagaactt aagcgaaatc
    11521 accggggaat ttacttcaga cgacctgctg gggcggattt tctccagctt ctgtattggt
    11581 aagtaaccgc gcttacgaag ccgtattctg actgtcagat gcggcttcgc ttcattgtta
    11641 gcactcctgt tattcctcaa cccttttttt aaacattaaa attcttacgt aatttataat
    11701 cttaaaaaaa gcatttaata ttgctccccg aacgattgtg attcgattca catttaaaca
    11761 attttagaat agacaaaatt cttgagtatt atactgtagc ctcgtgtctt tcgaggaata
    11821 agtgcattat gaatatctta catatatgtg tgacctcaaa atggttcaa
//
LOCUS       DAJTOR010000129        11837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTOR010000129 DAJTOR010000000
VERSION     DAJTOR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.129"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..192
                     /locus_tag="OO249_003056"
     CDS             <1..192
                     /locus_tag="OO249_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5637273.1"
                     /translation="RGAFFISALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGT
                     NTLLACCCLLRPEEVNSEY"
     gene            182..355
                     /locus_tag="OO249_003057"
     CDS             182..355
                     /locus_tag="OO249_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5637274.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(404..1476)
                     /locus_tag="OO249_003058"
                     /pseudo
     CDS             complement(404..1476)
                     /locus_tag="OO249_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1548..4292)
                     /gene="torS"
                     /locus_tag="OO249_003059"
     CDS             complement(1548..4292)
                     /gene="torS"
                     /locus_tag="OO249_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCR5637275.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPMLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSTVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4375..5364
                     /gene="torT"
                     /locus_tag="OO249_003060"
     CDS             4375..5364
                     /gene="torT"
                     /locus_tag="OO249_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5637276.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMIWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(5337..6029)
                     /gene="torR"
                     /locus_tag="OO249_003061"
     CDS             complement(5337..6029)
                     /gene="torR"
                     /locus_tag="OO249_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5637277.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQT
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGECYFLAADVC"
     gene            6149..7321
                     /gene="torC"
                     /locus_tag="OO249_003062"
     CDS             6149..7321
                     /gene="torC"
                     /locus_tag="OO249_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5637278.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            7321..9893
                     /gene="torA"
                     /locus_tag="OO249_003063"
                     /pseudo
     CDS             7321..9893
                     /gene="torA"
                     /locus_tag="OO249_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            9890..10489
                     /gene="torD"
                     /locus_tag="OO249_003064"
     CDS             9890..10489
                     /gene="torD"
                     /locus_tag="OO249_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5637279.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(10582..10887)
                     /gene="cbpM"
                     /locus_tag="OO249_003065"
     CDS             complement(10582..10887)
                     /gene="cbpM"
                     /locus_tag="OO249_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5637280.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10887..11807)
                     /gene="cbpA"
                     /locus_tag="OO249_003066"
     CDS             complement(10887..11807)
                     /gene="cbpA"
                     /locus_tag="OO249_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5637281.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2823 a         3061 c         3143 g         2810 t
ORIGIN      
        1 cgtggagcct tttttatatc tgccttattt ttcttcaacg ctgtatgtat agtaagcgat
       61 aacctgttga ttattgaatc tttcggggag atggcttata acatttctta cctgaccagg
      121 gtaccgggaa ccaacacctt actggcgtgt tgctgtcttt taagaccaga agaggttaac
      181 agtgaatatt gaagagttaa aaaaacaagc cgaaacggaa atcgccgact ttatcgcgca
      241 aaaaatcgcc gagctgaaca agaatacagg gaaagaagtc tctgaaattc gcttcaccgc
      301 acgagaaaaa atgaccgggc ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg
      361 tgaacatcac cccgtgcgag gtgatgttcc gcttgttgct aatttagtga tcaatcattg
      421 gcgcttgtgg aattaagcgt cggtacaatt cctccggcac cgggctttgc catactcccg
      481 catacattgc gtaaccaatc accgcgaaca taatccccag aaccagtagc gtcattaacc
      541 agccagacaa cgcaaaagct tttttatttg ccgcaggttt ttgcagtgaa aaggtcaatg
      601 ttgaggctac cggacatgac tctacgcaag tcatacagcc ggtacattcc actgttcgta
      661 cctgaattaa tttatctacc gggatccgtg atgggcaatt ttttgcgcat ttgccacagc
      721 cgatacaact ttcggcattg cgacgaatct taaacggcga taatagcgaa accacgccca
      781 tcagcgcgcc atatgggcaa agataacgac accaggcatg gcgaataaac aggctggcaa
      841 tcagcaaaac ggtcacgctg attaatgtcg cggtccccat atgacgaaag aaatcgagca
      901 tttttacgtc catcaccacg ctgtagggcg acaacataaa atagtgaatc gcctgagcgg
      961 gcatcaataa cgcgatatag agaaaaaaac tcaacagcaa atacttcacg ccgcgcagag
     1021 gaatatccag ccagcgggga aggacacatt gccgaccaaa cagtttgtta ccgagatcgc
     1081 cgattaattc agaaagcgta ccaaccgggc ataaccatga gcaaaaggcc tttttgagta
     1141 atagactgat gacgataaaa gcgaccaata acagcatcgc ggagcgtgga cggacggtaa
     1201 ctgacctgtt gtaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc
     1261 gatacctccc ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca
     1321 ataccagaac gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt
     1381 acgccaggtc gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg
     1441 ccgccgctgc cagcgggttc tttttttctc tgccatgatt ttgccagtcc gttaagttgc
     1501 ataccaaatg ccactatttt agttgttctt aactggctga tattgattca aatcgcgttc
     1561 aggtctttct tatgcaacca tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt
     1621 ggaagggggg cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcgatacgc
     1681 attcccaaac tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta
     1741 tcctgactgg cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat
     1801 aataccagcc attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc
     1861 gatacatcca gcgattgatc gttattgact tgcagttgga gatagtgcgc cagtaactga
     1921 ccgagcactt cacgcggcac cggtttaggg ataatcccgc ggaataatga actggtacgc
     1981 tggcgcagcg tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat
     2041 tgctgtgcca gttgtcgggc aagcgtaatg ccgtcgacat ccggcagatc aaaatccacc
     2101 agtgcggcag caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca
     2161 acagcaacaa cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc
     2221 gggttatctt caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt
     2281 tttggcacgg gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc
     2341 tccggcgtgc tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata
     2401 gtcagtccca gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt
     2461 tctgccagtt tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc
     2521 tcgccatcag tgcggctacg taaaacgatc tgcccttcgt cagtaaaacg cagggcgttg
     2581 ctcaacaggt tggttataac ctgacgaata cgtcgcggat cgcccattaa cgcggtcggt
     2641 acatcgtcgg caattgctgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt
     2701 aattgcaggg tactttccag cagcgggcgc ggttcaaagg gctcatcgct gaccgaaaca
     2761 ttcttgccac ctgcttcgat agccgaataa tcgagaatat cgttgaggat ggtcagcaac
     2821 gattcgccag agtcggtaat cgcccgcaaa tcatcacgct gggcgttaag tgcggggtta
     2881 tctgccagca gttgagcagt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc
     2941 atcgccgcca gaaaggccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgt
     3001 cggtgttcta tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc
     3061 tgttcacggt ggcgattcag tgcatgaaca gtgctgcgaa acgcatccat cagccgcccg
     3121 atggtatcca gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc
     3181 agcagccgtt gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc
     3241 acgcgccaaa ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg
     3301 tattgcccgc gtgcactggc tttttccaga tgcgccagtc cgtgctgatt acgcagctca
     3361 atggtgtcga ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt
     3421 tgtgcgagag tttgtaggtg attgctgatt tcactgtcct gctgatacag cgccagcaaa
     3481 tcgctatatt ggctaacggt agttaacgtt gttgcgacct gcgcacgaac acccggatct
     3541 tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc
     3601 attggtgcat ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc
     3661 agagcgctaa ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc
     3721 cgatcgagtg cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg
     3781 gcctgggtcg ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg
     3841 atctcatcgg cggcagcgac tatctgctga ctgagttgct gttgttgctg gcgtagttgc
     3901 agacgccgcc ccaccagttc cccttgctga cgtaacgaac gggagatctc ctgctcctgt
     3961 tgttcaatag cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc
     4021 ttcaggcttt gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca
     4081 ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc
     4141 atattcatgg taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc
     4201 aggttgtacc atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc
     4261 atccagagtc ttcgggtcag ggttaaattc acggtcggtg cactttaggt gaaaaaggtt
     4321 gagtcgcaaa gcagaatgca tctagcataa agccttatta ttgattaggc tatcatgcgc
     4381 gtactgctat ttttacttct ttcccttttc atgttatcgg cattttcggc tgataacctg
     4441 ttgcgctggc atgatgcgca gcattacacg gtgcaagcct ctacgccgct taaagccaaa
     4501 cgcgcatgga aactgtgcgc gctttatccc agcctgaaag attcatactg gttatcgttg
     4561 aactatggta tgcaggaggc aggcggctac agccagttgg ctacccagca agcacaaatc
     4621 gaccagtgta aacagtgggg cgcagaggcc attttgctcg gtagtagcac tacgtcattt
     4681 cccgacctgc aaaagcaggt agcaaatctg ccggtgatcg aactggtaaa tgctcttgat
     4741 gatcccctgg tgaaaagccg cgttggtgta ccctggtttc agatgggcta tcaacctggg
     4801 cgatatttgg tgcaatgggc gcacggtaaa ccactgaacg tgctgttgat gcccggaccc
     4861 gataatgccg ggggcagtaa agagatggtc gagggttttc gcgcagccat tgccggaagc
     4921 ccggtgcgta ttgttgatat tgcgcttggt gataacgata ttgaaatcca gcgtaacctg
     4981 ttgcaggaga tgctggaacg ccacccagaa atcgacgtcg ttgccggaac agccattgcg
     5041 gcagaggcgg caatgggtga agggcgtaac ctgaaaacgc cgcttaccgt ggtgtcgttt
     5101 tatctttcac atcaggtgta tcgcgggctg aagcggggaa gagtgattat ggctgccagc
     5161 gatcaaatga tctggcaggg ggaactggcg gttgagcagg ccatcaggca attacagggg
     5221 caatcggtgt ctgataatgt cagcccaccg attttagttc tgacgccgaa aaatgccgac
     5281 cgtgaacata ttcgccgctc gctgtcacca gggggatttc gtccggtcta tttttatcag
     5341 cacacatcag cggctaagaa ataacattca ccatgttgcg tcaccagtaa atccgcgctg
     5401 agtttatgac gtaaacgacg aattaacaca tcgacggtgc gcaggtcagg gttttccacc
     5461 cgacgcgcag aaagcatacg tagcagacgt tcacggctga gaatttcgcc cggattcgtc
     5521 acaaatgcca ccaacatttc atactctgcg cgggtcagtt taatcggctc gccatcccgc
     5581 tccagcgtat ggcgcgacac attcaggcaa taaccggcaa agcgatagca gttgtcctga
     5641 gtgtgcggtt gagcttgtcg cgcgaggtcg attcgccaga gcagattttt cacccgtact
     5701 accagttcgc gcagttccag cggtttggtg acgtaatcgt ctgcgcccat ttccagccca
     5761 acaatacggt caatccgatc gctgcgtccg gtaaccagaa taatccccac cgttgagcgt
     5821 tctcgcaggg cgcgggttaa catcaggcca ttttcatcgg gtaagttgat atccagcaga
     5881 attaaatcta ccggctgagt ctgcataatt tcccgcagcc cggcaccgct cgctgtaacg
     5941 gaaacggtat acccctcctg agtgaagtaa gattgtaatc gcgcctgggt aaccggctca
     6001 tcttcaacaa taacaatgtg atgtggcatc agagggtttt actcattctg ttcatattct
     6061 gccgtaagcc gttcatcctg accagtgccg ctgttcatat ttgctcatta agatcgcttc
     6121 actaaaccat aattttacag gggctattat gcggaaactc tggaacgcgc tacgccgacc
     6181 cagtgctcgt tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct
     6241 gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg
     6301 ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc
     6361 cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa
     6421 gcgcaaactg gaagcgagca atgatatcta ccagaccttt attgctcact ccattgatac
     6481 acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa
     6541 agaaaacaac tcggcaacct gccgctcctg ccataactac gatgcgatgg atcatgcgaa
     6601 gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat
     6661 cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca
     6721 gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat
     6781 taagccaatt tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc
     6841 ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac
     6901 ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc
     6961 ctcgattcgt ggtgatgtgc agcagcaggt aaaaacgctg gagaaaacca ccgttgccga
     7021 caccaatacc gagtggagca agttgcaggc cactgcgtgg atgaagaaag gtgatatggt
     7081 gaacgatatc aaaccgatct gggcttatgc ggattcgttg tacaacggca cctgtaacca
     7141 gtgccacggc gcaccggaaa tctctcactt tgatgctaac ggctggattg gcacgctcaa
     7201 cggcatgatt ggctttacca gtctcgataa acgtgaagaa cgcaccttgt tgaaatatct
     7261 gcaaatgaat gcgtctgaca tcgcaggtaa ggctcacggc gataagaagg aagaaaaata
     7321 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
     7381 ttaacagtcg ccgggatgct ggggccgtca ttgttaacgc cgcgcagtgc gactgcggcg
     7441 caagcggcgg atgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc
     7501 cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat
     7561 ccgtcgaaaa tgattgccgg attgccggat tgccggattg ccggattgcc ggattgccgg
     7621 atcacgtaca caacgcggcg cgtattcgtt atccgatggt acgcgtggac tggctgcgta
     7681 agcgccatct cagcgatacc tcccagcgcg gtgataaccg ttttgtgcgc gtgagctggg
     7741 atgaagccct cgacatgttc tatgaagaac tggaacgcgt gcagaaaact cacgggccga
     7801 gtgccttgct gaccgccagt ggttggcaat cgacggggat gttccataac gcttcgggga
     7861 tgctggcgaa agctattgcc ttgcatggta atagcgttgg tacgggcgga gattactcta
     7921 ccggtgctgc gcaggtgatc ctgccgcgcg tagtcggttc gatggaagtg tatgaacagc
     7981 aaacctcctg gctgctggta ttgcagaaca gcaaaaccat tatgctgtgg ggctccgatt
     8041 tgctgaaaaa ccagcaagcg aactggtggt gcccggatca cgatgtttat gaatattacg
     8101 agcaactaaa agcgaaagtc gccgccggtg aaattgaggt catcagcatc gatccggttg
     8161 tcacatccac ccatgagtat ctggggcgcg agcatgtgaa gcacattgcg gttaacccgc
     8221 aaactgacgt gccgctgcaa ctggcgctgg cgtatacgct gtacagtgaa aacctgtacg
     8281 acaaaaactt tctcgctaac tactgtgtag gttttgagca gttcctgccg tatctgctgg
     8341 gtgagaaaga cggtcagccg aaagatgccg catgggctga aaaactgacc ggcattgatg
     8401 ccgaaaccat tcgtgggctg gcgcggcaga tggcggcgaa cagaacgcaa attattgctg
     8461 gctggtgcgt gcagcgtatg cagcacggtg aacagtgggc gtggatgatt gtggttctgg
     8521 cggcgatgct ggggcaaatt ggcctgccag gtggtggttt tggttttggc tggcactaca
     8581 acggcgcagg cacgccgggg cgtaaaggcg ttattctgag tggtttctcc ggctctacgt
     8641 cgattccgcc tgttcacgac aacagtgact acaaaggcta cagcagcacc attccgattg
     8701 cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac ggtaaatcgg
     8761 taaaactgcc gccactgaaa atgtgtattt ttgccggaac taacccattc catcgccatc
     8821 agcagatcaa ccgcattatt taaggctggc gcaagctgga aacggttatc gccatagata
     8881 accagtggac ctcaacctgc cgctttgccg atatcgtact gcctgcgacc acgcagtttg
     8941 agcgtaacga tctcgaccag tacggcaacc actccaaccg tggcattatc gccatgaaac
     9001 aggtggtgcc gccgcagttc gaggcgcgca acgactttga tattttccgc gagctgtgcc
     9061 gtcgctttaa tcgcgaagaa gcctttaccg aagggctgga cgaaatgggc tggctgaaac
     9121 gcatctggca ggaaggtgta caacaaggca aaggacgcgg cgttcatctg ccagcgtttg
     9181 atgacttctg gaataacaaa gagtacgtcg agtttgacca tccgcagatg tttgttcgcc
     9241 accaggcatt ccgcgaagat ccggatctcg aaccgctggg cacgccgagt ggcctgattg
     9301 agatctactc gaaaaccatc gccgatatga actacgacga ttgtcagggg catccgatgt
     9361 ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc gcagaagtat ccgttgcatc
     9421 tgcaatctgt gcatccggat ttccgacttc actcgcagtt atgtgagtcg gaaactctgc
     9481 gtcagcaata tacggtagcg ggtaaagagc cagtattcat taacccgcag gatgccagcg
     9541 cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa cgctcgcggt caggtgttgg
     9601 caggggcagt agtttctgac cgctatgcac ccggcgtggc acgaattcac gaaggggcat
     9661 ggtacgatcc agataaaggc ggcgagcctg gtgcgctgtg caaatacggt aaccccaacg
     9721 tgctgaccat cgacatcggt acttcgcagc tggcgcaggc gaccagtgcg cacactacgc
     9781 tggtggaaat tgagaagtac aatggagcag tggagcaggt gacggcgttt aacggccctg
     9841 tggagatagt ggcgcagtgc gaatatgttc ccgcgtcgca ggtgaaatca tgaccacgct
     9901 gacagcacaa cagattgcct gtgtttacgc ctggctggcg cagttgttct cccgtgaact
     9961 ggacgatgaa caactgacgc aaatcgccag tgcgcagatg gcggaatggt tttcgttgct
    10021 gaaaagcgaa ccgccgctgg ctgcggcggt gaatgagctg gaaaactgta ttgccacgct
    10081 gacagtacgt gacgatgccc gtctggaact ggccgctgac ttttgcggcc tgtttttgat
    10141 gactgataag caggcggcgc tgccgtatgc atcggcctac aaacaggacg agcaagagat
    10201 taaacgcttg ttagttgagg ccgggatgga aaccagcggc aatttcaacg aaccggcaga
    10261 ccatctggcg atctatctcg agttgttgag tcatctgcat ttttcgctgg gagaggggac
    10321 cgttcctgcg cgaagaatcg acagtttgcg gcaaaaaaca ctgacggcgc tgtggcaatg
    10381 gttaccagag tttgctgcgc gttgccatca gtatgacagc tttggttttt acgcggcact
    10441 aagccagtta ttgctggtgt tagtggagtg cgaccaccaa aacaaataac gtcgtttatg
    10501 cgcctgaaaa gacgcgttta gcgtcgcatc aggcattatg gcgcagttgc cggatgcgtc
    10561 atcacatccg gcaaacacac atcacggatg agctacaaac cgggaaagcc gctggcgtag
    10621 caggcggttt tcctgcttca ggtgtgcaat atcatccatt aacgtcagcg ccaccgcgat
    10681 ccccggccag tccagagcca gttcatgacg caggcgtacc gcgcgttgca ccacaatggc
    10741 ggcatggtcg tcaaataccc aggttgtttc ctggatctca cgcggttcaa ccacccccaa
    10801 accgacaatt tcattcaact cctcttcaga gatgccggta tgcaggcaaa attcggtaat
    10861 agtaaaagtc accgtaacat tagccatcat gctttccccc aatctttacg tggatcaaaa
    10921 gacgactggg cgtctgccag ttgctgccac agcgcggcag tgttttcatc cggtttcggc
    10981 ggcatcacga ttttcagtac cgcatacaga tcgccggtct gttttttgct caccagacct
    11041 ttgcctttaa cgcgcaatcg ttgcccggcc tggctgcctg gcgggatagt cagcaaaatg
    11101 ctttctttca gtgttggaac ggtgacttta gcacccagcg ccgcttccca cgggctaacc
    11161 ggcaccacaa tttccagatc ctggccgaca atatcgaaca gcggatgtgg cgcaatatga
    11221 atcaccagcc acaaatcgcc atttggacca ccgttttcgc ccggcgtccc ctggcctttc
    11281 agacggatgc gttgaccatt gccgacgccc gccgggatct tcacattcag cgttttcggg
    11341 atttcctgtt cgatcatgcc aaaggcgtta taaaccggca ggttatagct gatggtacgc
    11401 ttatgctcag tcagcgtttc ttcgaggaat accgccactt cgatttcaat atcgtggccg
    11461 cgtgcggcgg ggcgttgacg gctctggcgg gcatgctgac cgaaaattga cgagaagata
    11521 tcgtcaaaat cttcggcatt aaaactctga ccgtcgccat gatggaactg acggttaaat
    11581 tgcggatcgt tgcgatgttg ccacatctga tcatactcag cgcgacgttg ttcatcactt
    11641 aacacttccc aggcttcagc gacctctttg aagcgggctt cggcatccgg ttctttgctg
    11701 acatcaggat ggtatttacg ggctagtcga cgataggcgg tcttgattgt cttgagatcg
    11761 tccgtcggtt tcacgcccat gatggcgtaa taatccttta attccatagc gttatctcgc
    11821 gtaaatcaac acaaatt
//
LOCUS       DAJTOR010000130        11748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTOR010000130 DAJTOR010000000
VERSION     DAJTOR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.130"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            440..1096
                     /gene="pphA"
                     /locus_tag="OO249_003067"
     CDS             440..1096
                     /gene="pphA"
                     /locus_tag="OO249_003067"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCR5637282.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1097..1288)
                     /locus_tag="OO249_003068"
     CDS             complement(1097..1288)
                     /locus_tag="OO249_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5637283.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1393..1629)
                     /locus_tag="OO249_003069"
     CDS             complement(1393..1629)
                     /locus_tag="OO249_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5637284.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1747..3186)
                     /gene="rsmF"
                     /locus_tag="OO249_003070"
     CDS             complement(1747..3186)
                     /gene="rsmF"
                     /locus_tag="OO249_003070"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR5637285.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3266..5899)
                     /gene="yebT"
                     /locus_tag="OO249_003071"
     CDS             complement(3266..5899)
                     /gene="yebT"
                     /locus_tag="OO249_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR5637286.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLNNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVSGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISATGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5868..7151)
                     /gene="yebS"
                     /locus_tag="OO249_003072"
     CDS             complement(5868..7151)
                     /gene="yebS"
                     /locus_tag="OO249_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5637287.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7281..7778
                     /gene="msrC"
                     /locus_tag="OO249_003073"
     CDS             7281..7778
                     /gene="msrC"
                     /locus_tag="OO249_003073"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5637288.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7875..8573
                     /gene="proQ"
                     /locus_tag="OO249_003074"
     CDS             7875..8573
                     /gene="proQ"
                     /locus_tag="OO249_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5637289.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8593..10641
                     /gene="prc"
                     /locus_tag="OO249_003075"
     CDS             8593..10641
                     /gene="prc"
                     /locus_tag="OO249_003075"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5637290.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            10833..11714
                     /gene="htpX"
                     /locus_tag="OO249_003076"
     CDS             10833..11714
                     /gene="htpX"
                     /locus_tag="OO249_003076"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5637291.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
BASE COUNT         3015 a         2894 c         3034 g         2805 t
ORIGIN      
        1 cgtgccacgg ctggctgatg gatgttcgat agcgcgagtt tgaatgaaaa tcagccggag
       61 atgattttac ataattgcta cggaattatt caatacagga attgcttgcg tctgcatgga
      121 ttgacctgaa attttcccga aaatttctct aaaaaactcg aaaaaaatgg taactggttg
      181 aatgtattaa tatgcaatgg tacgtgtcag ggattaaaag atgaacgtaa atttattcaa
      241 cgcattaatt ttaaagggtt ttattgtttg ttgacgaaaa caggaatcgt gttcggtctc
      301 tttttatctg ttaaaagcca gaagcatttc cttcgctgac tttatagtca accataacac
      361 acactctact gtctgagtcc agcgtttttt aacattcttg ttaagattat gtgatcttta
      421 gcacgggagg aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa ttgcgggtca
      481 tcaatggcga catatctggc tttctggcga tatacacggt tgtcttgagc agttgcgccg
      541 caaattatgg cattgtcgtt ttgatccgtg gcgagattta cttatctcag tgggagacgt
      601 tatcgatcgt gggccgcaaa gtttacgttg tctgcagtta ctggaacaac attgggtttg
      661 tgcggtaaga ggcaatcatg aacagatggc gatggatgcg ctggcatccc agcagatgtc
      721 tttgtggttg atgaatggcg gcgactggtt tattgcgctg gcagataatc aacagaaaca
      781 agcgaaaacg gcgctggaaa aatgtcagca tttgcccttt attcttgaag tacacagtcg
      841 caccggcaag catgttattg ctcatgcgga ttatccagat gatgtttatg aatggcaaaa
      901 ggacgttgat ttgcatcagg tcttgtggag ccgctcgcga ttaggtgaac gccaaaaagg
      961 gcagggaatt acaggtgctg atcatttctg gtttggtcat acaccgttgc gacatcgcgt
     1021 ggatattggc aacctgcatt atattgatac cggtgctgtc tttgggggcg aactgactct
     1081 tgtgcaattg caatgattaa aaatcaccgt actcctgtgc aggtcgccag aaaccatcta
     1141 taaaatcctc aatcggaaaa caaccgccct ggcggatccg ttgatcgctc atagaataaa
     1201 gacactgctg ttccgtgttg tagacatcca caacaatatc ttcacaaccg ccatccaggt
     1261 agcaaacaaa aagtaccagc gcgaacattt catccccgaa gtgtggtgcc gtaccgttaa
     1321 gtttaggaga gattttacaa cgggggaata accaggacaa ataacccgcc atgccgacgg
     1381 gttctttttg gatcaggcaa gacgcataat ccaggcatca gattgttgat cgtaacgggt
     1441 acggtaaagg acagagtgtt cgccatttag cagggcaggg atactggttt cagcatccca
     1501 ggcatcgagt tgcatacata aatcggggtc agatttacaa ggaatggtta acgttcgatc
     1561 acccggcgat tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact
     1621 ggttttcatc ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca
     1681 ccatttttca gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt
     1741 gcgctgtcag gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct
     1801 gtttttcaat cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac
     1861 caacacgtca tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc
     1921 ctccgcttcc tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat
     1981 aactgcttca tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa
     2041 gcgagaaaat cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc
     2101 tttgtcacgc tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc
     2161 ctggcgaatt tgtccagctt cgcgatcttt caccgggctg aacggaaaat taccgacttt
     2221 gtatttgggg gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa
     2281 gccttcgcag tcgtaaattt gtgggaaaac atgcaaaaag ccttcttcgg tcagcgcttt
     2341 gtttgcacca gggaagagat cgccaagtgg taaaaactct actgcgtcgg ggtaagtctc
     2401 tttcagccac aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac
     2461 cagcgtacca ccaggacgta atgcatgaaa ggcgctgtcg ataagctccc gttgtgtagc
     2521 tgcgatttcc tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac
     2581 cacgccttcg ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc
     2641 cgcaccaaac acgcggccat caaaatgtgt gagcgcaaca ttactgatgc cacagcggct
     2701 gatattggca tgtaacactt ttacccgact ggcggaaaac tcattggcaa ggattgcccc
     2761 ttcgttattc atccgcgcgg caatttgcgt cgtttttgag cctggcgcag cagcgacatc
     2821 catcacccgc tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact
     2881 ggcttcctga atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc
     2941 atcttcattg tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt
     3001 ccagccataa ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac
     3061 gcgaatgctg cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa atgagagcgt
     3121 cgaaggcata gcttcgcgca tttgtgtcag aaacgcgtcc gggaaataaa cggtgtgttg
     3181 ggccacgagc atgtaccact ggcaaaaaaa caggcgcgca gtttagcata aacgctccgg
     3241 cgcaggcacg ccggagcagt gggcattatt tgggaagcgc agttccccat tcacgccact
     3301 ctttcggttc actttcctgc aacaggaagt gtttgccttc ctgggctttc ggtgccagtg
     3361 gcgtacccgg aggcgtggcg aaggcgatgc cgccacggat aaactggtta aaggtgccgg
     3421 ttttcactac gccgcccgtc agaccaaagt ccagactgta acccgatgcc aaccagaaga
     3481 cggaattgtt acgcaccagg tgttgatagc gtttactgat gcgcatcgca atcatcacgc
     3541 gatctgacaa tgtccccagc gtcattccgg taaccgtacc tacttccaga ccacggaaca
     3601 gcacaggcgt accaatacct aacgaaccgg cttccggtgc ttcgacaata atgcttaagc
     3661 catccaggta acgcgaatca gtaatagtag cttcttgcaa ctcaaagtca cggcgaggat
     3721 taccccgacc tggttcgaca ttgatatacg gctggaggat agtatctaga tgctcaacgc
     3781 ccgtggctga aatttgcggt gtgaccaccg agaagcgcgt accgccgcgg gcgaaggtct
     3841 ggacatactc cggatagagc accgcctttg cctgtacttc attgcgcgcg gtaatcagat
     3901 ccagcgtctg gatttgcccg atatcaatac cgagatagcg aattggcatc ccgaccgcca
     3961 gttttccggc atcgaaagcg tgaagcgtaa tctgcccact aaccgcacgg gccgctgttt
     4021 cggaagcata cagaattcgt ttgtcgcctt tacgctgact ggcgctggca ccgctgaggt
     4081 tatcgaagct aatggctccc tttaatgctc tggagagcgg ggatgcctgt acggtcaggc
     4141 cactaccatt cagctgaact ttcgccccgc cttctgccca gaacacgcta ttgctggtca
     4201 gaaggttgcg atactccggc ttaatatgca gatcgatatc aaacgcgtta gctcgcggac
     4261 gcacggtaat cacttcacca acttcaaatt tacggtacag cactaccgat cctgcctgca
     4321 catccggcag cgtctctgca ctcaaactca cggttgtggt gggtaaatcg ctaaggctgt
     4381 tctccagcgc tttttccaga ttggcataca gtggatagct ggctttcatc tcacctttat
     4441 cgcctggcag aatacgtatc ccgccgttaa tccattctga ggcgctggca ccaagaaact
     4501 caacgccatc cagccccacc ttcacgtcga cacggctgtt gacgacaaat ttgctatcgc
     4561 cttttaccag ttcgcgatgc tgaggctcga tggcgacggt aaaggtaacg cctttgctgg
     4621 tgagtttacg atcaataacc tggcctacct gcacgccgtg aagaatgagc ggctgacccg
     4681 tatcaatacc gtaactttcc ggcgcggtca gggtcagcgt cagaacatca ggttcatgca
     4741 gcagtgcttt ttcgcctggc acaacaacga actctttgcg tggctcgcca tcgccgggta
     4801 ccaactcgaa ggttttgcca gtcagcaggg cgctgagatt ggcatcgcta agggataatt
     4861 tcggattacg taattcgatg cgggtatttt cacgaagcag ggtaacgacg ctgggatcaa
     4921 ctgtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtt agctgtccga
     4981 cttccagtcc ctgatacatt aatggcgtcg agtcggcggt taatccggcc ccactcggca
     5041 gttccagttt gattattacg ccacgctggc tatgggcgag gtcttcatac agaccaaagg
     5101 tatcttccgc ctcggcaggt ttcgactctt ctggtgaatc gaaggcaatc gcaccgttaa
     5161 ccagtgccgc caggctttcc agtttcacct tcgcgccact gatactgaca ttggcatcaa
     5221 cgccggaaac gttccagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     5281 tcaggacatc aatcaccacg ccttgtttgt tgggattgat ggcatagtcg tagacttttc
     5341 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agatcggggg
     5401 cttgcaggtg gatcatcaga tcgccattgt tcagccgata tttcggttgg gtatcgagtg
     5461 cgacaaagtg atcctgctct ttacctgtac ccggcatcat gccgatatag ttcccaccga
     5521 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcacc agccagaatt
     5581 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     5641 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     5701 gaacaggcgt acggcccggg acaataccat ccgccgacat aaagtcgatg gtgacggtat
     5761 tacttcggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     5821 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     5881 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5941 agaatagtca aaattacggc tgcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     6001 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc
     6061 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     6121 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     6181 accagaatac tggcgataaa cacgattccg gcgaccgcaa tgttgctact tgccagcgac
     6241 ataatcccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     6301 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     6361 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gcgaccacgc
     6421 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     6481 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     6541 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     6601 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     6661 attacccact ctttaagacg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     6721 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     6781 cagaaaaaga ccatcgcccc cgttatcgta tcgccctgtt tggtcatttg ccagatgcct
     6841 tgcatgacgt ttgcgtcgat acgaatacct aacagccaga tatgcaacag cggttcgccc
     6901 caggcaaacg gcatcagcaa cagcatggtg aaagccattg ccgccaggcg cgttagcgac
     6961 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ctgatgagaa
     7021 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     7081 ggcagttcct cgccgattga cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     7141 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     7201 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     7261 gacaaacaag tgattatata atgaacaaaa cagaatttta cgcggattta aatcgcgact
     7321 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     7381 tgttatacga gcgtctcact gacgtaaact gggcaggttt ttatttgctt gaggacgata
     7441 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     7501 gggtgtgcgg cactgcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     7561 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     7621 tgaaaaatca gattattggt gttcttgaca tcgatagtac cgtcttcggt cgctttacag
     7681 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     7741 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     7801 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     7861 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7921 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7981 tcggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     8041 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     8101 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     8161 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     8221 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     8281 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     8341 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     8401 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagctctg aaggtgaaag
     8461 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     8521 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     8581 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     8641 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     8701 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     8761 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     8821 ctgctcgatt acagccacaa cgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     8881 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8941 aatctggcgc aaaagcgccg ttttgagcgt taccagtacg ctttgtcggt actggaaaag
     9001 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     9061 aaaaacgagg ctgagttgaa cgcgctgtgg gacagtaaag tcaaattcga cgagttaagc
     9121 ctgaagctgg caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     9181 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     9241 gcgtttgcgc gtgaaatcga cccgcatacc aactatcttt ccccgcgtaa taccgaacag
     9301 ttcaacactg aaatgagttt gtcgctggaa ggtattggcg cagtgctgca aatggatgat
     9361 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     9421 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt
     9481 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     9541 ctggaaattt tacctgctgg taaagggacc aagacccgca ctgtaacatt gacccgtgaa
     9601 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     9661 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     9721 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     9781 ggtggggcgt tgactgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     9841 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9901 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9961 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
    10021 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
    10081 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
    10141 ggcagtacgc aacgtaaagg cgtaacgcca gacatcatca tgccgacggg taatgaagaa
    10201 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat tgatgccgcg
    10261 acttatgtga aatcaggcga tttaacggcc tttgggccgg agctgctgaa ggaacataat
    10321 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
    10381 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
    10441 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg cgaaggtaaa
    10501 ccggagttga agaagctgga tgatctaccg aaagattacc aagagccgga tccttatctg
    10561 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
    10621 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
    10681 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
    10741 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
    10801 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
    10861 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10921 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10981 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    11041 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcag
    11101 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    11161 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    11221 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    11281 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    11341 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    11401 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    11461 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg
    11521 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    11581 aaccgcaaga agcaaccagc atgatggctt tctgcattaa tggtaagtcg aaatcgctca
    11641 gtgagttgtt catgactcac ccgccgctgg ataaacgaat tgaagctctg cgtacgggtg
    11701 aatacctgaa gtaatatgaa gtaaatacta aaaagtgcgt cctgatcg
//
LOCUS       DAJTOR010000131        11291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTOR010000131 DAJTOR010000000
VERSION     DAJTOR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.131"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..416
                     /locus_tag="OO249_003077"
                     /pseudo
     CDS             1..416
                     /locus_tag="OO249_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            587..934
                     /locus_tag="OO249_003078"
     CDS             587..934
                     /locus_tag="OO249_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5637292.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            924..1286
                     /locus_tag="OO249_003079"
     CDS             924..1286
                     /locus_tag="OO249_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5637293.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            1283..1780
                     /locus_tag="OO249_003080"
     CDS             1283..1780
                     /locus_tag="OO249_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5637294.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(1788..2972)
                     /gene="emrD"
                     /locus_tag="OO249_003081"
     CDS             complement(1788..2972)
                     /gene="emrD"
                     /locus_tag="OO249_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5637295.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(3391..3480)
                     /gene="tisB"
                     /locus_tag="OO249_003082"
     CDS             complement(3391..3480)
                     /gene="tisB"
                     /locus_tag="OO249_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5637296.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            4045..4143
                     /gene="ivbL"
                     /locus_tag="OO249_003083"
     CDS             4045..4143
                     /gene="ivbL"
                     /locus_tag="OO249_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5637297.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            4249..5937
                     /gene="ilvB"
                     /locus_tag="OO249_003084"
     CDS             4249..5937
                     /gene="ilvB"
                     /locus_tag="OO249_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5637298.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQDIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            5941..6231
                     /gene="ilvN"
                     /locus_tag="OO249_003085"
     CDS             5941..6231
                     /gene="ilvN"
                     /locus_tag="OO249_003085"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5637299.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            6305..6895
                     /gene="uhpA"
                     /locus_tag="OO249_003086"
     CDS             6305..6895
                     /gene="uhpA"
                     /locus_tag="OO249_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5637300.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            6895..8397
                     /gene="uhpB"
                     /locus_tag="OO249_003087"
     CDS             6895..8397
                     /gene="uhpB"
                     /locus_tag="OO249_003087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5637301.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            8407..9726
                     /gene="uhpC"
                     /locus_tag="OO249_003088"
     CDS             8407..9726
                     /gene="uhpC"
                     /locus_tag="OO249_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5637302.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            9864..11255
                     /gene="uhpT"
                     /locus_tag="OO249_003089"
     CDS             9864..11255
                     /gene="uhpT"
                     /locus_tag="OO249_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5637303.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
BASE COUNT         2489 a         2933 c         3195 g         2674 t
ORIGIN      
        1 ccacgcaatg atagtcattg aggatttgaa ggtcagcaac atgtcaaagt cagcagcggg
       61 tacggtcagc ctgccggggc gcaatgtccg ggcaaaatca ggtttaaacc gttcgatact
      121 ggatcagggc tggtatgaaa tgcgccgcca gcttgagtac aagcagctct ggagtggcgg
      181 tcaggtgctt gctgttccgc cagcgttgcg cgtgctgtgg tcatacagcg aaagaaaatc
      241 gcctgtcaca aagtaaattc agatgccagg tatgtggata tacagcgaac gccgatgtaa
      301 atggcgctcg taacatttta gcggcggggc acgccgttct tgcctgtgga gagatggtgc
      361 agtcaggccg cccgttgaag caggaaccca ccgaaatgat tcaggcgaca gcctgaacgt
      421 agcagggatc ctcgtccttc agggcgtgga ggatgtcaat ttgcgctatg atacccatct
      481 gacctccttg tagcggatgg caaaagcata tcaggaaacg ggcggacgaa attcttaaaa
      541 aaaaccgcta aagatagagc gttagtgaaa ttgcaaagga gcaacaatga aaatttcccg
      601 ccttggagaa gcgcccgatt accgcttctc gctggctaat gagcgtactt ttctggcgtg
      661 gatccgtacc gcactaggat ttctggcggc gggggttggg cttgatcaac ttgcgccaga
      721 tttcgccacg cccgtcattc gtgaactgct ggcgttgttg ttgtgtctgt tttccggcgg
      781 gttagcaatg tatggctacc tgcgctggtt acgcaatgaa aaagcgatgc gtctgaaaga
      841 ggacctgccc tacaccaaca gcttattaat cattagctta atattgatgg tcgtggcggt
      901 gatcgttatg ggactggtgt tgtatgccgg atagccgcaa agccagacgc attgccgacc
      961 caggactgca gccggagcgc acatcactgg cgtggtttcg taccatgctg ggctacggcg
     1021 cgttgatggc gttggctatc aaacacaact ggcaccaggc gggcatgtta ttctggattt
     1081 cgattggcat tctcgccatc gtggcgctga tcctctggca ctacacccgt aatcgcaatt
     1141 taatggatgt cacgaatagc gatttttccc aatttcacgt agtccgtgac aaatttttga
     1201 tctccctcgc ggtgttatct ctcgcaatac tgtttgctgt aacgcatata catcaactta
     1261 tcgtatttat tgagagagtc gcatgacagg aagtcaggtc attgatgctg aagaggacag
     1321 gcataaactg gttgtagagt acaaggatgc tctgcaacct gccgattttt atcacaattt
     1381 caaacagcgg ggcatccgct ccgtacagct cattccctat cttgaatttg atgaccgggg
     1441 cgatctgacg gccgcttcgg tgacagcaga gctgtggggg aaatttttaa ttgccctgtt
     1501 cgagtgttgg gtgcgggcag atatcagtcg tatctcgatt gagctttttg acgccacctt
     1561 acaaaaatgg tgtggcggcg aaaacccgca accgcgccgt gactgccagg cgtgcgactg
     1621 gcatcgcctg tgcccgcacg ctcggcagga gacgccagac agcgtgcttt gtgcgggcta
     1681 tcaggcgttt tattcttact ccgcaccgca tatgcgggtg atgcgtgatc tgataaagca
     1741 gcatcgttct cccatggagc tgatgacgat gctgcggtga cgtgcgctta aacgggctgc
     1801 ccctgatgcg acatccgcgt cgccagcggc agccagcaca gcacgatcaa caatcccatt
     1861 aaggtcatca ataaccccag gctaccctga ccggtttgcg gcaacatcgc agagagcgac
     1921 gccagcacgc cggaaccaat gttttgcaga ccgccgacca gcgcgccagc cgtgcccgcc
     1981 aggaagggga acggctccat cgcgccgctg gtcgccagcg gaaacagcat cccggcaccg
     2041 aaaaagaaca gcgcggcggg aacgagcagc gtccagacat tcatcacgcc aaaccagtcg
     2101 gggatccaca tcagcaagcc agccagcagg cagcagataa ccgactgcca cattaacgtg
     2161 gagaagcgtt tattgggacg tccggcaaac catgcgccaa aaaacgctgc cggaatcggc
     2221 agaataaaca aaatactgac cgtcatactg ctcagcccta acaccgcgcc catcagcacg
     2281 ccggagcagg cttcaaaggc ggcaatcccg gccagaccgc caatcagcat cagcaaataa
     2341 cagttaaaac cgctgttacc gaaaagcgtt ttataactgg taagcaggcg cgtgcgcggt
     2401 gcatcgaccg gacgcgtttc cggcatccag cgggccatac tgaaggtcac accaacacaa
     2461 agaaccaaca aaaagagata acaggcgcgc cagttccaca tcgtatccag cagaccgccg
     2521 attagcggtg cgagcaacgg actgacgaga atccccatgt ttaacaggct gttagcatgg
     2581 cgcaactgtg tccgttcata taaatctcgc ggcaaagtac gcgccattac gccgccaacg
     2641 ccggtgccca tcccctgcat cgcgctggcg gcaatcaata ccgtcaaact ggaggtcgtg
     2701 accgcgacca gcgttgccag cataaaaatg gacattccga cgaggatcac cggtcggcga
     2761 cccacacggt cggaaatcgg gccataaaac agctgtgaga caccgtaagt cagcagataa
     2821 gcgcccatta cgctctgcac cgccccttca cggacgttga gatcgcgcgc catatcggca
     2881 atagctggaa tataaatggt ttgcgccatc tgaccgacgg ccacgagtaa taccaacatc
     2941 aataacaaat tgacgtttct ttgccttttc attatcacgg atgcttttat aaaaataatt
     3001 agaataagtc actacgcatt cccataaatg cgggagagga atctatcaca ttgatgaatg
     3061 ttagccagat atacgccagg aatggggaat tgtttagcgg cagaatatgt aaacaaaagc
     3121 ggcaataaat gttgtcggga gggattacgt gcgaactgta accctctccc caaaggggcg
     3181 agggggaaac caccgttccc tttcagaaaa tgagctaaac attgcacggg acggtcccct
     3241 cgcccctttg gggagagggc tagggtgagg ggcacaggca gcgcaggtgc aaacattctg
     3301 ctggtagaag aagcccccaa ctccaacccg cacgctaaat ataaaagggg agcggttacc
     3361 cgctcccctt tggtgcgact tgaatctgaa ttacttcagg tatttcagaa cagcatcaag
     3421 cagttgcagt gctgcaacaa tgagtttgag gataagaatg gcgatatcca ccaggctcat
     3481 acgcgtctcc tgtggttcag gagcactgcc agctgacgta cctttccgct gcctgttgcc
     3541 agctgttgtg cacgcggtat ctcaaggaga aacgttgtgc ttgttgacat aacacagtgt
     3601 gctcacgcgg ggttaaggcg ctgacggcac cacccgtttc agccaggact tagtgcgccg
     3661 ggtaacgatg cggcccccgc ataatacatt gcgtacagtg atattataga agtttactgt
     3721 ataaataaac agtgatattt ggacaaaacg caaactgtga tcgatactca actatgccct
     3781 aaatacgtca aaattcgtgc cgaaattgcg cgttctgcgc ggaacacgta tactttcagt
     3841 gttgacataa tacagtgtgc tttgcggtta ccagctgcag gcggctgatg aaacctcgct
     3901 ccggcggggt tttttgttac ctgcaattca gtacaaaacg tgatcagccc ctcaattttc
     3961 ccttgctgaa aaattttcca ttgtctcccc tgtaaagctg tgcttgtata aatattgtta
     4021 aacacaaaac caacaaggtc cccaatgact acttccatgt tcaacgcaaa actactacca
     4081 actgcgctat ccgccgcagt ggtcgtcgtg cgtgtggtgg tggtcgtcgg caatgcgccg
     4141 tagggactgg aacaacacac gattccaaaa ccccgccggc gcaaaccggg cggggttttt
     4201 cgtttaagca cctcccggaa agtcggccca gaagaaaagg actggagcat ggcaagttcg
     4261 ggcacaacat cgacgcgtaa gcgctttacc ggcgcagaat ttatcgttca tttcctggaa
     4321 cagcagggca ttaagattgt gacgggcatt ccgggcggtt ctatcctgcc tgtttacgat
     4381 gccttaagcc aaagcacgca aatccgccat attctggccc gtcatgaaca gggcgcggga
     4441 tttatcgctc aggggatggc gcgcaccgac ggtaaaccgg cggtctgtat ggcctgtagc
     4501 ggaccgggtg cgactaacct ggtgaccgcc attgccgatg cgcggctgga ctccatcccg
     4561 ctgatttgca tcaccggtca ggttcccgcc tcgatgatcg gcaccgacgc cttccaggaa
     4621 gtggacacct acggcatctc tatccccatc accaaacaca actatctggt cagacatatc
     4681 gaagaactcc cgcaggtcat gagcgatgcc ttccgcattg cgcaatcagg ccgcccaggc
     4741 ccggtgtgga tagacattcc taaggatgtg caaacggcag tttttgagat tgaaacacag
     4801 cccgctatgg cagaaaaagc cgccgccccc gcctttagtg aagaaagcat tcgtgacgca
     4861 gcggcgatga ttaacgctgc caaacgcccg gtgctttatc tgggcggcgg tgtgatcaat
     4921 gcgcccgcac gggtgcgtga actggcggag aaagcgcaac tgcctaccac catgacttta
     4981 atggcgctgg gcatgttgcc aaaagcgcat ccgttgtcgc tgggtatgct ggggatgcac
     5041 ggcgtgcgca gcaccaacta tattttgcag gaggcggatt tgttgatagt gctcggtgcg
     5101 cgttttgatg accgggcaat tggcaaaacc gagcagttct gtccgaatgc caaaattatt
     5161 catgtcgata tcgatcgcgc agagctgggt aaaatcaagc agccgcatgt ggcgattcag
     5221 gcggatgttg atgacgtgct ggcgcaattg atcccgcagg tggaagcgca accgcgtgca
     5281 gagtggcacc agttggtagc ggatttgcag cgcgaattcc cgtgtccaat cccgaaagcg
     5341 tgcgatccat taagccatta cggcctgatc aacgccgttg ccgcctgtgt cgatgacaat
     5401 gcgattatca ccaccgatgt gggtcagcat cagatgtgga ccgcgcaagc ttatccgctc
     5461 aatcgcccac gccagtggct gacctccggt gggctgggca cgatgggttt tggcctgcct
     5521 gcggcgattg gcgcggcgct ggcgaacccg gatcgcaaag tgttgtgttt ctccggcgac
     5581 ggcagcctga tgatgaatat tcaggagatg gcgaccgcca gtgaaaatca gctggatgtc
     5641 aaaatcattc tgatgaacaa cgaagcgctg gggctggtgc atcagcaaca gagtctgttc
     5701 tacgagcaag gcgtttttgc cgccacctat ccgggcaaaa tcaactttat gcagattgcc
     5761 gccggattcg gcctcgaaac ctgtgatttg aataacgaag ccgatccgca ggctgcattg
     5821 caggatatca tcaatcgccc tggcccggcg ctgatccatg tgcgcattga tgccgaagaa
     5881 aaagtttacc cgatggtgcc gccaggtgcg gcgaatactg aaatggtggg ggaataagcc
     5941 atgcaaaaca caactcataa caacgtaatt ctggagctca ccgttcgcaa ccatccgggc
     6001 gtaatgaccc acgtttgtgg cctttttgcc cgccgcgctt ttaacgttga aggcattctt
     6061 tgtctgccga ttcaggacag cgacaaaagc catatctggc tactggtcaa tgacgaccag
     6121 cgtctggagc agatgataag ccaaatcgat aagctggaag atgttgtgaa agtgcagcgt
     6181 aatcagtccg atccgacgat gtttaacaag atcgcggtgt tttttcagta accgctcaag
     6241 gcttgaacaa catcgcgctt atcgttaagg taagcgcgtt ttttttaccc gccaggacaa
     6301 gaccatgatc accgttgccc ttatagacga tcacctcatc gtccgctccg gctttgcgca
     6361 gctgctgggg ctggaacctg atttgcaggt agttgccgag tttggttcgg ggcgcgaggc
     6421 gctggcgggg ctgccggggc gcggtgtgca ggtgtgtatt tgcgatatct ccatgcccga
     6481 tatctccggt ctggagctgc taagccagct gccgaaaggt atggcgacga ttatgctctc
     6541 cgttcacgac agtcctgcgc tggttgagca ggcgcttaac gcgggggcac gcggctttct
     6601 ttccaaacgc tgtagcccgg atgaactcat tgctgcggtg catacggttg ccacgggcgg
     6661 ctgttatctg acgccggata ttgccattaa actggcatcc ggtcgtcagg acccgctaac
     6721 caaacgtgaa cgccaggtgg cggaaaaact ggcgcaagga atggcggtga aagagattgc
     6781 cgccgaactg ggcttgtcac cgaaaacggt acacgtccat cgcgccaatc tgatggaaaa
     6841 actgggcgtc agtaacgacg tagagctggc gcgccgcatg tttgatggct ggtgatgaag
     6901 acgttgttct cccgcttaat taccgttatt gcctgctttt ttatcttctc tgccgcatgg
     6961 ttttgcctgt ggagtatcag cctgcatctg gttgagcgcc ctgatatggc ggtgctgtta
     7021 tttccgtttg gtctgcgtct ggggctaatg ctgcaatgcc cgcgcggata ctggccggtg
     7081 ctgctgggcg cggagtggct gctgatttac tggctaacgc aggcggtcgg tttaacccat
     7141 tttccgttat tgatgatcgg tagtttactg acgttactgc ccgtagcgct gatctcgcgc
     7201 tatcgccatc agcgtgactg gcgcaccttg ctgttacagg gggcggcgtt aacggcggcg
     7261 gcgttgttgc aatcgctgcc ctggctttgg cacggcaaag agtcgtggaa tgcgctgttg
     7321 ctgactttaa ctggcggcct gacgctggcc ccgatatgtc tggtgttctg gcactatctc
     7381 gccaataaca cctggctgcc gctcggcccg tcactggttt ctcagccaat caactggcgc
     7441 gggcgacatc tggtctggta cttgctgctg tttgttatca gtctctggct ccagttggga
     7501 ttgccggacg aactgtcgcg ctttacgcca ttctgtctgg cgctgccgat tatcgcgctg
     7561 gcctggcact atggttggca aggggcgctg attgcgacgt tgatgaacgc catcgcgctg
     7621 atcgccagtc aaacctggcg cgatcatccg gtggatttat tgctctcgct gctggtgcaa
     7681 agtctgacag ggttgttgct tggcgctggc atccagcggt tgcgtgaact taaccagtcg
     7741 ctgcaaaagg aactggcgcg caatcagcat ctggctgaac ggttgctgga aaccgaagag
     7801 agcgtgcgcc gtgatgtggc gcgtgagctg catgatgata tcggtcagac catcactgct
     7861 attcgtactc aggcgggcat tgttcagcgg ctggcggcag ataacgccag cgtgaagcag
     7921 agcgggcagc tcatcgaaca actatcgctg ggcgtttacg acgcggtgcg ccgtttgttg
     7981 ggtcggttac gtccgcgcca gttggatgat ctcaccctgg agcaggccat ccgctcactg
     8041 atgcgggaaa tggagctgga agggcgcggt attgtcagcc atctcgaatg gcgaatcgat
     8101 gaatcagcgt taagcgaaaa ccagcgcgtg acgctgtttc gtgtctgcca ggaagggctg
     8161 aacaacattg tgaaacatgc tgatgccagc gcggtcaccc tgcaaggctg gcagcaggat
     8221 gaacggttga tgctggttat tgaagacgat ggcagcggtt tgccgccggg ttccgggcaa
     8281 caaggttttg gcctcaccgg aatgcgcgag cgcgtaacgg cgctgggtgg cacattacac
     8341 atttcctgtc tgcacggcac gcgtgtcagc gtttctctac ctcaacgcta tgtctaaggt
     8401 ttgatgatgt tgccgtttct gaaagcgcct gccgatgcgc cattaatgac tgataaatat
     8461 gaaattgatg cccgctatcg ctactggcgt cggcatattc tgctgaccat ctggctgggt
     8521 tacgcgctgt tttacttcac gcggaaaagt tttaacgccg ccgtaccaga aatccttgct
     8581 aacggcgtgc tcagccgtag cgatatcggc ctgttagcga ccctgtttta cattacctat
     8641 ggcgtgtcga agtttgtctc cggcattgtc agcgatcgct caaatgcccg ttattttatg
     8701 gggatagggc ttatcgccac gggcattatc aacattctgt ttggcttctc gacgtcgcta
     8761 tgggcgtttg ccgtactctg ggtgctgaac gcctttttcc agggctgggg ttcaccggtg
     8821 tgtgcgcgtc tgttaacggc ctggtattca cgtaccgagc gcggtggttg gtgggcatta
     8881 tggaacacgg cgcataacgt cggcggcgca ctcattccca ttgtgatggc agcggctgca
     8941 ctacattacg gctggcgtgc cgggatgatg attgctggtt gtatggcgat cgtcgtggga
     9001 atttttctct gctggcggct gcgcgatcgc ccgcaggcgt taggtttacc ggcggtcggt
     9061 gaatggcgac acgacgcgct ggagatagct caacaacaag aaggggcagg gctgacgcgt
     9121 aaagagatcc tcaccaaata tgtgttgctg aatccgtata tctggctgct ttcgttttgc
     9181 tatgtgctgg tctatgtggt ccgggcggcg atcaacgact ggggcaattt gtatatgtcc
     9241 gagacgctgg gcgtcgatct ggtcacggcg aatacggcag tgacgatgtt tgaactgggt
     9301 ggatttatcg gtgcgctggt agcgggttgg ggctcggaca aattgtttaa cggcaaccga
     9361 gggccgatga atttgatttt cgccgccgga attttgcttt cagtcggctc cctgtggctg
     9421 atgccatttg ccagctacgt gatgcaggca acctgcttct tcaccattgg tttttttgtc
     9481 tttggcccac agatgttaat cggtatggcg gcggcagagt gttcccacaa agaggcggca
     9541 ggggcggcga cggggtttgt cggcttgttt gcttatctgg gggcgtcgct tgctggttgg
     9601 ccgctggcga aagtactcga tacctggcac tggagcggat tttttgtggt tatctctatc
     9661 gccgccggga tttccgcact gctgttactg ccctttttga acgcccagac accgcgcgaa
     9721 gcgtgatgca tctcaccttt tcacttcata tccggcaaaa ctaagaaatt ttccaggttt
     9781 tgcctggacg ctatctcagg cctgatttgc tgctgatttt tacaatgcat gcctcacgca
     9841 ggtattcatt tcaggagtaa cccatgctgg ctttcctaaa ccaggttcgc aagccgaccc
     9901 tggaccttcc gctcgaagtg cggcgcaaaa tgtggttcaa accgttcatg caatcctacc
     9961 tggtggtctt tatcggctac ctgacgatgt acctgattcg caagaacttt aacatcgcgc
    10021 agaacgatat gatttcgacc tacgggttga gcatgacgca gctggggatg atcggcctgg
    10081 gtttctccat cacttatggc gtgggtaaaa cgttggtttc atactacgcc gacggcaaaa
    10141 acaccaaaca attcctgccg ttcatgctga tcctgtctgc catttgtatg ctgggcttca
    10201 gtgccagtat gggcagcggc tcggttagcc tgttcctgat gattgccttc tacgccttaa
    10261 gcggcttttt ccagagtacc ggcggttcgt gcagttactc caccatcacc aaatggacgc
    10321 cgcgtcgtaa acgcgggaca ttcctcggtt tctggaatat ttctcacaac cttggcggtg
    10381 caggcgcagc aggtgtggcg ctgttcgggg caaattacct gttcgatggc catgtcatcg
    10441 gcatgtttat cttcccgtcg attatcgcgc tgattgtcgg ttttatcggc ctgcgttacg
    10501 gcagcgactc cccagaatct tatggcctcg gcaaagctga agaactgttc ggcgaggaga
    10561 tcagcgaaga ggacaaagag acagaatcta ccgatatgac caagtggcag atctttgttg
    10621 agtatgtgct gaaaaacaaa gtgatctggc tgctgtgctt cgccaacatt ttcctctatg
    10681 tggtacgtat tggtatcgac cagtggtcaa ccgtatacgc gttccaggaa ctgaaactct
    10741 ctaaagcggt ggcgattcag ggctttacgc tgtttgaagc tggtgcgctg gtcggtacgc
    10801 tgctgtgggg ctggctctct gacctggcga acggtcgccg tggcctggtg gcctgcatcg
    10861 cgctggcgct gattatcgcc acgctcggtg tgtatcaaca tgccagcaac gaatatatct
    10921 atctggcttc tctctttgcg ttgggtttcc tggtctttgg cccgcaattg ttgattggtg
    10981 tggctgctgt tggctttgta cctaaaaaag cgattggcgc tgccgatggt attaaaggca
    11041 cctttgctta cctgattggt gacagctttg ccaagttagg tctgggaatg attgccgatg
    11101 ggacgccggt attcggcctt accggctggg caggcacctt cgccgcactt gatatcgccg
    11161 cgattggttg tatctgcctg atggcgatag tggcggtgat ggaagaacgc aaaatccgcc
    11221 gcgagaaaaa aattcagcag ttgacagtgg cataaacgta actggtgact tttgcccggc
    11281 atgacgccgg g
//
LOCUS       DAJTOR010000132        11172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTOR010000132 DAJTOR010000000
VERSION     DAJTOR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.132"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            63..661
                     /locus_tag="OO249_003090"
                     /pseudo
     CDS             63..661
                     /locus_tag="OO249_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(690..1178)
                     /locus_tag="OO249_003091"
     CDS             complement(690..1178)
                     /locus_tag="OO249_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5637304.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(1191..3998)
                     /locus_tag="OO249_003092"
     CDS             complement(1191..3998)
                     /locus_tag="OO249_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5637305.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVAFGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGCFYDKD"
     gene            complement(4036..4140)
                     /locus_tag="OO249_003093"
     CDS             complement(4036..4140)
                     /locus_tag="OO249_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR5637306.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVS"
     gene            complement(4149..4523)
                     /locus_tag="OO249_003094"
     CDS             complement(4149..4523)
                     /locus_tag="OO249_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5637307.1"
                     /translation="MKDIDTETRNNTVADNVTAVEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            complement(4579..5089)
                     /locus_tag="OO249_003095"
                     /pseudo
     CDS             complement(4579..5089)
                     /locus_tag="OO249_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            complement(5089..6273)
                     /locus_tag="OO249_003096"
     CDS             complement(5089..6273)
                     /locus_tag="OO249_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCR5637308.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIADKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKNGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            6431..7540
                     /locus_tag="OO249_003097"
                     /pseudo
     CDS             6431..7540
                     /locus_tag="OO249_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            7789..10908
                     /locus_tag="OO249_003098"
     CDS             7789..10908
                     /locus_tag="OO249_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5637309.1"
                     /translation="MIGQFLSATEILAKNYVRNKMVKNPFYSNLKWNFIEKNIIRLTS
                     SPVKSVLCISAFSFVLLYVGYLNELFIKNNLLHYFPFRHSLTEWQTTILSGQLTIIGI
                     VYPLVIGLVSVLFQKKADRKIAQTAYQRYSGFMLAGLSGLFLSGFILLSVLIKTVFGS
                     YLYGIACLISILWLLINIVLSIWFFIVSLEILDDVKRQIIIKRYIAFEIVMPHICNKI
                     SAKLRLYPIYQKHNYSNLEITQADYKGEYISVASSYSKEDELSLYHRPFQLTLNLINY
                     QLKKKNHFASFVIGDNRTKETESTGKILFSVKNIKPDSLLIKILKQCFYRAPIKGGDF
                     SVSLTMQAITADTYMYLRDSDLISFDNAISALINNFNNLCDLYFFQDDNTNNNFLLIT
                     TELFERSFQYEFSDEVYKISNNSMDKINLSERFFELCLWSGVRIINNRKHLISNELCI
                     YMGITRSQWSILTEWFRNNQSLLNASLRSRYNRILRTYITVWEQYQESINFRFCNTEN
                     SDLFELFCKTQLQELPSMIIDATQTRDPSTIDTAVDLINRWQHSMNIDSHSVEKYSYK
                     GQLFNPGFFISKKLNFNSDREWFNIAIINALTDMRICTCLYLTSRINTSDKLMTHYIK
                     LILEGKLIDQTGGYETPTEEIDNASQLIKILIRICLWTWSENMEHNGWMNSLARRLRD
                     YDKTDMVMGRVYSNVFDCGFIDMEQSWVQLLLIFSNKNDSVSKEIKEAIENNYITYRE
                     KQRLIGVLSKICNSIEYTKIKLTLTLDDLQTKKENLRKLLQEHINMLKKDLDMRLQDA
                     AIDVHRLDSTARKTSEHLRKRIKKTLPLSLFKSIDFKQASDCFTKHKISIKIDKEPYA
                     EGIESIPYINEGDIQADLILKDIQRIILSNLFSTGCSQHTVIEDFNMLIDHIKSSADL
                     AGKLVLVMSKEIFQQYNRMLFDNPNLRELMRKNDDGSMNITTESGTRKVYFLPFVNQP
                     FSLVVKDNYFTKLIIREYDNNKLVNVTSENIKSDSDKFKLTLNYELNIVFEGNADLKI
                     AHSQRVTSE"
     gene            11044..>11172
                     /locus_tag="OO249_003099"
     CDS             11044..>11172
                     /locus_tag="OO249_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5637310.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFST"
BASE COUNT         3058 a         2792 c         2507 g         2815 t
ORIGIN      
        1 ttaaataatg gccgtttctg gcctgcatgg catgatttgc gcttttggac gggagattca
       61 gtgtgctgat tggctatgta agggtatcaa caaatgacca gaatacagac ctgcaacgaa
      121 acgctcttgt ttgtgcagga tgtgaacaaa tatttgaaga taaattaagc gggacaaaga
      181 cagaccgacc gggattaaaa cgcgctttaa agcgccttca aaaggtgaca cgctggttgt
      241 ctggaaactg gatcgcctcg ggcgaagcat gaaacatctg atttctctcg tcggggaact
      301 acgggagcga gggattaatt ttcgcagtct gaccgacagc atagacacat cttctccaat
      361 ggggcgtttt ttcttccacg tgatgggtgc cctggctgaa atggaacgtg aattaattgt
      421 tgaacgtaca ctggccgggc tggcggcagc gcgcgcaagg gggcgcacag gcggacgtcg
      481 accgaagctg acaaaagaac agcatgagca aatagcaagg ctaatcaaaa acgggcacga
      541 cagaaaacaa ctggcaataa tttacggcat tggcacatcg acgatttatc gttatcaccc
      601 tgtaggcgat atacagactg aagaaacaac cgggcagact caggaaaatg aaaaccgcta
      661 atctgaccat tagcggtttt tgtgttaaat cagaacagcc ctttaactga actggccgcg
      721 ctgttaagag atgatgtcac cttatctttg aagccggaca gcatatcact gaacgatgag
      781 gattgcaggc gctcccgcaa atcctcatca cagcgttcaa gagtcagtga aaattctatc
      841 tttttcgcct taccgtagcg atcaaactcg gagcgggtcg tattcgtttc agtcaggaca
      901 tacatgccgt aaatctgccc gacgccatca atcaaaggcc agggtcgtcc tgtatacgcc
      961 tgcgtagtca gcagcgacag cgacacttcg ccacctgtaa tttcaggata aagcacacca
     1021 gaaagaacga tgcgatcatc acctgcaccg atatactgcc agcttgctga acggttaacg
     1081 cgttcatttt tcacatgccg ccagcttttg ttttgctgta actgctgatg cggcagcgtg
     1141 cgcagctcaa aaacaaacat gccgtagatc atcatcatgg ccatgactcc tcaatcttta
     1201 tcgtaaaaac agccacgccc ggcacgggcg cgccgttcca tttctgccct gaccatttca
     1261 ccgaccagtt tcgccagttc gcggggattc tgcgtaacaa cgttatgcag atgaacatga
     1321 atttcaccac caaatccgaa ggcaacaggc tcccggttac gggaagttac aggaactgat
     1381 gccactggag atcgtatagc ctccgccacc gggcgggagc tggccgcaac aacagggacc
     1441 agcgccggag gcagcggagc cggaaccacg ggtgtgatat taattgcggg ggcaggctta
     1501 ctgacctgcg caatcttccg ctcctgccac tccccacgaa cagcaagtgc tcggggcagg
     1561 tttttaaaga caatatcgcc ggggccaatg cgttttttcg tctcctcaac cagcttacct
     1621 gtgttatcag caattttgct gagtctgcgc agcgtaccgg tattgctgtc tgtgagcggt
     1681 ttattgtctt tggggttatc acctccggtg ccattgccat tttccacagg cttcggcgga
     1741 ttgattttcg ccagatcccc ctgaagcaag gcaaccttgt cctgaagaat ggccgcacgc
     1801 tgtgcgtctt cgattttctt gcgcgccctt tccgcttcat ccggaagcac accaagcttt
     1861 tcaagtatcc acgccagcgt atccagcaac atttttgcag gtgtcagaac aagttgtaac
     1921 gcaccgccaa gaacgttacc gaatatctcg ccagcactgg tacatttatc cagagtttcc
     1981 ttgctggact ccatcggtga cagcagcgat ttaaaccagt taaacacctg gctgatccca
     2041 ctcccgattg cgtcaaaaac aggaccaaac cgttcaaagg tttcgcgcaa cggggtcagc
     2101 ctttccataa tcccgctgaa caccccggca aaaaatgccc tgatgggatc ccagtatttc
     2161 cagataagaa cggcagctcc ggcaagcgca gccacgataa gaccaaccgg actgaacaac
     2221 gccccgatag cgcctcccag taaagaaacg gaacccgtca ccattcccca tagtgctggc
     2281 agaaccctga cagcattcat tgatccggtc aggagggaaa aaccaagacg cagttttgcc
     2341 agcgggccag caagcacacc aatagccagc gacaacgagc caaccgttgc agtcattgcc
     2401 agcaatgcac cgcctgcaat cagtagctgg cgcgtcagtg cgggatgggc ctgcgccagc
     2461 gccgtcacct ttgataccac acgcgtgagc cactgcgtga cagaacgcag cggaccgtca
     2521 atcagatctg caatgcggat gcgcaaccct tcccatgcac tgctgagtga tttcagatcg
     2581 ccgtcaaggt tgttggccat aacctttgcc gtgcgttcag cctcaccgcg cgcgccttca
     2641 agttcttttc tcagtttggg taaggagccg tcacctgccg catcaacgag ggccataaat
     2701 gatgtgaaag cctcttctcc ggcaatgtcc ttaaagaacg atacccggtc aacttccccg
     2761 tatttgcggg tagctttata aaggtcagcc agcacatcct ccatcgggcg cattttgccc
     2821 ccggcatccg agacagacac gccaagctct ttcagcgcct ctgctgccgc ctttggcggt
     2881 gatgccagac gagccaggct ggcacgcatt gccgtaccag catcactccc cctgataccc
     2941 atattcgcca gcacgcccgc catcgctgcg gcctgctcca gagatattcc cagcttaccc
     3001 gccaccggac ctgcatattt catggtttca cccagtgcgc gaaggtcagt gttggtacgg
     3061 gtaaacgctg cggtgagtgt gtcgccgacc cggtccatct ggtcagcaga aagaccgaac
     3121 tgcgtcagaa tatttgagcc aatatccgcc gtctcaccga ggtccatacc gccagccgtt
     3181 gccatgctca gtacgccagg gagcgcagcc tgaatggcct gtggtgtaaa gccagccatt
     3241 gcaagaaatg cctgtccact ggcggcatcg cctgcggtga actgcgtttc agagccaagt
     3301 tttaacgcct gctcacgcag cgccttaaac tgcgggctgt tctggtcgat tcgcgtcagt
     3361 gcctgaacgc gggacatctc tttgccgaac ccgatcgcag gctgcaaaaa acgcccggca
     3421 gcatagccgc ccgccgctgc cgcaccaatt gccagcgcac cacctgtttt cagttttccc
     3481 gctgtttcct gcgcgcgcga ataccgctca cgcgcccgtg ttacacgcgc aagcgcctgc
     3541 cgttcgcgtt caagctggtt gttgtactgt tcggtgcgtc tgatggcctg ctggatggtg
     3601 ttatcgctgc ctgtcaggga aatgccgtgg cgtttcagct ctccgccaag ctcccgcatt
     3661 ttctgaattt cccgtgtgcg cgattcattc aggcgttcaa gccgggtgct taactgctgc
     3721 atcagctttt gttgtttttc gctgagcact gtacccgtgc gttgtaactg attaagggcg
     3781 ttaagctggc gtcgtgcttt cacgatgcca gcatccgctt tactgacagc gtcacgggcg
     3841 cgctcaaatg atcgcgcctg acgctcgaga tttttgatcg ccccctgcgt tcgctggatg
     3901 gagtcaccaa actgccccat caggcggcgg gcgttttcgg caggccgggt cagcctgtca
     3961 acggcgctga aagcgacccg gatatcaaga gtcttcattg tctgcattcc cgctacgaag
     4021 tgccgcccgc tcacgtcagc taaccacttc gccgggcgtc atcatgaaga tttcggcggg
     4081 cgaccagtta aaaatggcgg caatatccgc caccagatct tcgatgtgct caaagcacac
     4141 cagggtgatt acgctgccgt ctcctgcacg ctcttcgcgc cagagtctgg ctcgctcata
     4201 aaatttacag ccacagcgca caactgaata aaatcgcgtg acgacatttt tttaatcatc
     4261 acttcatcca gtcgtggcga ggtcacgcga ggcaacagcg tgaacatggt atccgctttc
     4321 agattcagca catcagacag cgacagacca cgcagggatc cagcctgctc aatagccccg
     4381 gtgatctcca catacgtgat tttttcgcca ccacgctcaa ttggtcgggt cagttttacg
     4441 ccacgttcga cagccatatc ctcaactgcc gtcacattat ccgccacggt gttattccgg
     4501 gtttcagtat cgatgtcttt catctgttgt ctccttttca gtcagaggcg acgcactgcg
     4561 ccgccagcat attacttatc agccaagccc aagcgcggaa cggatgcgat cgggcacaat
     4621 gtccttgccg tccttccggt aaatgaagtt cagcaggtca atctcccaca acgggcgatc
     4681 gttaacactc agcttgtagt aggtgtttta atggcgtaag tgtgtgatgt ggcttcgccc
     4741 tgtttggctt cccccatatc aatttccgtc acacgtccgc gcatttcgac ttcatacagg
     4801 tcgctttctg catcggtgta gtattcaccc gcaaaacgca gcagcgtgcc gtcaatcgtg
     4861 ccgccatact taaggaacag ctcacgaact gcgcccccat gacaaagctc gcatcaagcg
     4921 cggagtcgtc cagaccgaga tcaatactta ccgcacccat catgccacca ccccggtagc
     4981 tgtcggtttt gcgcgtcagc ttaggcagag tgacggacgt cacattaccc acttcgtttt
     5041 caccatccac aaacagcgta aaaaagcgaa gatgttttgg cacagccatc aggcacctcc
     5101 cagcaccgca aatgcgggtt caaagtattc atcagtaaac gtctggtaaa gctccatgtc
     5161 ttccagtggc ggaacgggcg tatatttgta gcgaatacgc acacgtccct gacgtaaatc
     5221 cgtggtgctg ttatccacca cgtcatacca gcactccgcg ccaatcagtt tcccggcagt
     5281 caccagtgaa tccagttttg ccctgatggc actgataaca tccttcacat tcgcaggcgt
     5341 cagtggactg tcgatggttt caaactgcgc ttccgcaatt gaatcagcca gcacctgtgc
     5401 ggttcgggta tacacctcaa agatgtaggc gttcgtttcc ggtgtgcggt taccccagaa
     5461 gcggaacccg ttgcgacgaa taatggtcgt gatttctttg ttgttgaggc tgttggcatc
     5521 gctgtcttcg gcctgcaacg accagaacac atgcctcgac atccccagca catttttaac
     5581 cggaacgttg gacagcgatt tgtgccatcc ctgttcatgg tcaatgtacg cacgaaggcc
     5641 gcacgcatag gcaggcgcgg ggaacgtttc gtttttgcca tttttcgggt tgtaggcgat
     5701 gaagtccggc cataagagca tcacctcacg ttcgttgaat ttctggcggt aggtaattgc
     5761 ctcggccatc gtgttacagc catgacatga ggcatacaca aacgcgcgca gtttatctgc
     5821 aatcacgcac agggattttg ttaccgcctc cgtgtccagc tccggcgcgg ccagaatacg
     5881 cggacggtat ccgatgcttt catcctgctc tgcaacaagc agcgcataca tccccgtata
     5941 gctgccgtca tcctcagaac caccgataac cagttgatcc tgcgtctttc cgtcttcttc
     6001 tttgtgttca gccacgcgaa cgacgatcac ctttgtgctc acctggtctg cgatggcctt
     6061 aagcgcacga taaagcgtcc ccgttgtccc gcattttccc agcacgtcat tgacgcgggt
     6121 cagcagtgtg ggcttgttca gcgggaacag cttcgcgtcc gcatcatccg ccgttgccac
     6181 gataccgata acgctggaat caacatcgtt aatcgctgtt accaggtcgg tattctccgt
     6241 aacacgggca ccatgaaaac gagtttcact catagcttca gccccttgta tccgttaaat
     6301 gattcagcaa caatcatcac ccaccacgcg cgtaatctca cccctgcgcc gttctcccgc
     6361 cacggcgaca acaaaaagca gtaaccccct ccgcacgcac atgcgaccat gccgcacagg
     6421 gagggagcag atgaccgaca ccaccatgca attgctcagt cagggcacgg cccccgtgaa
     6481 aatgccggat tttgatattc tctcggaggg taaaacgctg tcaggcgtgg cggagcgcct
     6541 gatgagcctg taactgaccg acaaccgggg atttgaggcg gaccagctca ccatcacgct
     6601 ggatgatgcg gatggtcagt tgcagctacc gccacggggc gcgcgtctga cggttctcat
     6661 tggctggaaa ggggaaccgc tgacagaaaa aggcacttac attgttgatg aaatcgctca
     6721 cgaaggaccg ccggacaggc tgactgtttc agccagaagc gcagattttc gggatgaatt
     6781 taacgttaaa cgtgaggtgt cctggcatga tgtgaccgtt gagcgtgtgg tatccgccat
     6841 cactcatcgg tacggtctga aaccgcaaat cagcgaaatg ctgatggata tcgaaatcga
     6901 ccacgccgac cagaccgaag aaagcgacat gtcctttctt acgcgcatgg cggaaatgct
     6961 gggagcaatc gccacggtaa aaagcggcaa tctgttattc atcatgccag gtggtggcgt
     7021 gaacgcacag gggcagccgt tgccctcgtt cgccattaca cgcagcagcg gcgatcgcca
     7081 tcagttccgc attgctgacc gcgaggcgta tacgggggta cgcgcctact ggcttgatct
     7141 taattacggg aaaaagaaaa aagtcagcgt gaaacgccgc aaaccgccaa aacccaaaaa
     7201 ggagaaaagc agcagtcggg aaggcgacta tatggaaggt gcggaaggca acgtgtttgt
     7261 gttacgcaag acttatcaga acgagcaggc agcaagacgc gcagcggcgg caaagtggca
     7321 gcagctacaa cgcggagccg catcattctc catcacgctg gcgcgtggtc gtgcagaact
     7381 ctatcccgaa atgcatggca cagtgacagg ctttaaaagc gagattgatt atcaggactg
     7441 gactattgca aaagccgagc acaccattga taacagcggc tttaccacgc agcttgaact
     7501 tgaagcaaaa atcccggaat ggatagcaga aacagaataa atctcttaaa aatattattt
     7561 ttgagatgtg cttctacact gacagtaaga acaaaagata atgtatcagc ccactagatg
     7621 tcagaggttg gggcatcaac cagatgctaa caataatacg gatgtttttc ccacatgaca
     7681 gaggaagtca agtataatct tcacagtcca tcccgtccaa gggaatatga ctcaatagta
     7741 aaagttccag tttagttagt agacccacac tttatagaag agtaattcat gattggacaa
     7801 tttttaagtg caacagagat tttagcaaag aactatgttc gtaataaaat ggttaaaaat
     7861 ccattttatt cgaatttaaa atggaatttt attgaaaaaa acatcataag attaacatcc
     7921 tcaccggtta aatcagtact ttgcatatca gctttttcat ttgtactact ttatgttgga
     7981 tatttaaacg aattatttat aaaaaataat cttttacatt atttcccgtt taggcattca
     8041 ttaaccgaat ggcaaacaac cattttaagt ggccaattga caattattgg tattgtatac
     8101 ccccttgtta taggtttagt aagtgtctta ttccaaaaaa aggcagatag aaaaatagcc
     8161 caaactgctt atcagcgtta ctcaggattc atgcttgctg gactcagcgg gcttttctta
     8221 tcaggattta tacttctcag tgtactaatt aaaactgtct ttggaagtta tctttatggt
     8281 atagcttgtc taatcagcat attatggctt ttgataaaca tagttctttc tatttggttc
     8341 tttatcgtga gtcttgaaat tcttgatgat gtaaaacggc aaataattat aaagcgatac
     8401 attgccttcg aaatagtaat gccccatatt tgtaacaaaa tatcagccaa gcttaggtta
     8461 taccctatct atcaaaaaca taactattca aatttagaaa tcacgcaagc cgattacaaa
     8521 ggagaataca tatctgtggc tagcagttat tctaaggaag atgagttaag tttatatcac
     8581 cgtccttttc aacttactct taatcttatt aattaccaac taaaaaagaa gaatcatttt
     8641 gcgtcatttg tcattggtga taatcgtaca aaagaaacag aatccacggg taagatactt
     8701 tttagcgtta aaaatatcaa accagacagc ttattaataa aaatacttaa gcaatgtttt
     8761 tatagggctc cgattaaagg aggagatttt tccgtaagtc ttacaatgca ggctataaca
     8821 gcagatacgt atatgtatct gcgggattct gatctcatta gttttgacaa tgctatctct
     8881 gcattaatta ataactttaa taatctctgc gatttgtatt tttttcaaga tgataatacc
     8941 aacaataact tcttactgat cactacagaa ttatttgaac ggagttttca atatgagttt
     9001 tcagatgaag tttataaaat atcaaataat tccatggata aaattaatct ttctgaaaga
     9061 ttttttgaat tatgtctttg gagcggagtg cgtattatca ataatagaaa acatcttatc
     9121 agcaatgaac tttgtatcta tatgggaatt actcgttcac aatggtcaat attaacagaa
     9181 tggttccgaa acaaccaatc actcttaaat gcctcgcttc gttcacgtta taatcgtata
     9241 ttaagaacct atatcactgt ttgggagcaa tatcaagaaa gcataaactt tcgtttctgc
     9301 aatacagaga attcagattt atttgaatta ttttgcaaaa cgcaactaca agaacttcca
     9361 tcgatgatta ttgatgcaac acagactcgc gatccttcta ctattgatac agccgtcgat
     9421 cttattaacc gatggcagca ctcaatgaat attgacagcc attcggtaga aaaatacagt
     9481 tacaaaggcc agctttttaa cccgggtttt ttcatatcca aaaaactaaa cttcaattca
     9541 gacagagagt ggttcaatat tgccatcata aatgcgttga ctgacatgcg tatatgcaca
     9601 tgtctttatc tgacatcaag aatcaatact tctgacaaac taatgacaca ttacattaaa
     9661 cttattttag agggtaaact tattgaccaa accggtggat atgaaactcc cactgaagaa
     9721 attgataatg caagccaact aatcaagata ttaattagaa tctgcctgtg gacatggtct
     9781 gaaaatatgg agcacaatgg gtggatgaat agtttagcaa ggcgtttacg tgactatgat
     9841 aaaacagaca tggtgatggg cagagtttac agtaatgttt ttgactgtgg atttattgat
     9901 atggaacaat catgggttca gctattatta attttttcta acaagaacga tagcgtatca
     9961 aaagaaataa aagaagcaat agaaaacaac tatataacat atcgtgagaa gcaacgactc
    10021 attggagtat tatcaaaaat ttgtaactcc atcgaataca ctaaaatcaa gctgacttta
    10081 acattggacg atctccaaac aaaaaaagaa aacttaagaa agttgcttca agagcatatt
    10141 aacatgctaa agaaagatct tgatatgaga cttcaggatg ctgccataga tgtacatcga
    10201 ctagatagca ctgcacgtaa aacatcagaa catcttcgta aacgcatcaa gaaaacgctc
    10261 cctctttcac ttttcaaatc tattgatttt aagcaggcta gcgattgttt cacaaaacat
    10321 aagatatcta ttaagataga taaagaaccg tatgccgaag gaatagaatc catcccttat
    10381 atcaatgaag gtgatattca agcagactta attctcaagg acatccagag aataatactt
    10441 tcaaatttat ttagcactgg ctgttcgcaa cataccgtaa tagaagattt caatatgttg
    10501 attgatcaca taaaatcatc tgctgacttg gctggcaaac tggttcttgt gatgagtaaa
    10561 gagatctttc agcaatataa tcgtatgctt ttcgataatc ccaacctcag agaactaatg
    10621 agaaaaaatg atgatggctc tatgaacata actactgaat ccggtacccg taaggtttac
    10681 ttcttaccat ttgttaatca accattttct cttgtggtaa aggataacta cttcactaaa
    10741 ttaattatta gagaatatga taataataag ttagtaaatg tcacttcaga gaatatcaaa
    10801 agtgactcag ataaattcaa gctaactctt aactatgagt tgaatatagt atttgaagga
    10861 aacgctgatt taaaaatagc tcactcgcaa agagtgacat cagaataata tctttgacgg
    10921 cgctccttag agtgccgttc gtttattaac taatgctaaa aatcattata tagagaaagt
    10981 catgtttaaa tcttagagaa tacaacctag aatagtgtaa gcactacaat aagagaggtc
    11041 gctatgttcc gttgtccgct ttgtggcgca tctgcccgta tccgcaccag tcgtccggaa
    11101 aatgattcaa acaccgtgag acaaaagtat tatcagtgta acaatctaga atgcggcgta
    11161 tgtttctcaa ca
//
LOCUS       DAJTOR010000133        11097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTOR010000133 DAJTOR010000000
VERSION     DAJTOR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.133"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            90..1067
                     /gene="csiD"
                     /locus_tag="OO249_003100"
     CDS             90..1067
                     /gene="csiD"
                     /locus_tag="OO249_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5637311.1"
                     /translation="MNALIAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKPLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1087..2355
                     /gene="lhgO"
                     /locus_tag="OO249_003101"
     CDS             1087..2355
                     /gene="lhgO"
                     /locus_tag="OO249_003101"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5637312.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFNDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRATRSVDALHAAFNQ"
     gene            2378..3813
                     /gene="gabD"
                     /locus_tag="OO249_003102"
                     /pseudo
     CDS             2378..3813
                     /gene="gabD"
                     /locus_tag="OO249_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            3827..5093
                     /gene="gabT"
                     /locus_tag="OO249_003103"
                     /pseudo
     CDS             3827..5093
                     /gene="gabT"
                     /locus_tag="OO249_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5331..6692
                     /gene="gabP"
                     /locus_tag="OO249_003104"
     CDS             5331..6692
                     /gene="gabP"
                     /locus_tag="OO249_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5637313.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYVPVKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTDLLAIGIICTVPIMAA
                     GIS"
     gene            6751..7413
                     /gene="csiR"
                     /locus_tag="OO249_003105"
     CDS             6751..7413
                     /gene="csiR"
                     /locus_tag="OO249_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5637314.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7414..7863)
                     /gene="kbp"
                     /locus_tag="OO249_003106"
     CDS             complement(7414..7863)
                     /gene="kbp"
                     /locus_tag="OO249_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5637315.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(7947..8105)
                     /locus_tag="OO249_003107"
     CDS             complement(7947..8105)
                     /locus_tag="OO249_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5637316.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8288..8587
                     /locus_tag="OO249_003108"
     CDS             8288..8587
                     /locus_tag="OO249_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5637317.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8597..9121
                     /gene="ygaP"
                     /locus_tag="OO249_003109"
     CDS             8597..9121
                     /gene="ygaP"
                     /locus_tag="OO249_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5637318.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDRIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9168..9572)
                     /gene="stpA"
                     /locus_tag="OO249_003110"
     CDS             complement(9168..9572)
                     /gene="stpA"
                     /locus_tag="OO249_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5637319.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            10240..10689
                     /gene="alaE"
                     /locus_tag="OO249_003111"
     CDS             10240..10689
                     /gene="alaE"
                     /locus_tag="OO249_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5637320.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(10726..11070)
                     /locus_tag="OO249_003112"
     CDS             complement(10726..11070)
                     /locus_tag="OO249_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5637321.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
BASE COUNT         2616 a         2816 c         2953 g         2712 t
ORIGIN      
        1 catttttcag aaatgtagat atttttagat tatggctacg aaatgagcat cgccatgtca
       61 ccctacatct cataagagga tcgcttctga tgaatgcact gatcgccgta caaaataacg
      121 ctgtcgattc aggccaggac tatagcggat tcactctcat cccgtcggcg caatccccgc
      181 gtctgctgga actcaccttc accgaacaga cgaccaaaca gtttctcgaa caggttgccg
      241 aatggcccgt gcaggcgctg gagtacaaat cgtttctgcg ttttcgggta ggcaaaattc
      301 ttgacgatct gtgtgcgaat cagctgcaac cattgctgtt gaagcccctg ctaaaccgtg
      361 ctgaaggtgc gctgttgatc aatgcggtgg gtatcgatga tgtggcgcag gcagatgaga
      421 tggtgaagct ggcaacggcg gtagcgcatc tgattggtcg ctcaaatttc gatgcgatga
      481 gcggtcagta ttacgcgcgt ttcgtggtga aaaatgtcga taactcagac agctatctgc
      541 gtcagccgca ccgcgtgatg gaactgcaca acgacggcac gtatgtcgaa gagatcaccg
      601 attacgtgct gatgatgaaa atcgatgagc aaaacatgca gggtggaaat tcgttgctgc
      661 tgcatctcga tgactgggaa catctggacc actatttccg ccatccactg gcgcgtcgcc
      721 cgatgcgctt tgccgcgccg ccgagcaaaa acgtcagcaa agatgttttc catccagtgt
      781 tcgacgttga tcaacagggt cgcccggtga tgcgctatat cgaccagttc gtccagccaa
      841 aagattttga agaaggcgtg tggttgagcg agctttccga tgccattgaa accagcaaag
      901 gcattctttc tgtacccgtt cccgttggca aattcctgtt gataaacaac ctgttctggc
      961 tgcacggtcg cgaccgcttt actccgcacc cggatctgcg ccgtgaactg atgcgtcagc
     1021 gtggctattt cgcttacgcc actcaccact accagacgca tcagtaagcg caaaggaatt
     1081 gagcggatgt atgattttgt gattattggc ggcggcatca tcggcatgtc gaccgccatg
     1141 caactgattg atgtttatcc ggatgcccgc attgcgttgc tggaaaaaga gtccggcccg
     1201 gcctgtcacc agacgggcca caacagcggc gtgatccatg ccggggtcta ttacacgccc
     1261 ggcagcctga aggcgcagtt ttgcctggcg ggaaaccgcg ccaccaaagc cttttgcgat
     1321 caaaacgaca ttcgctatga caactgcggc aagatgctga tcgccacgtc cgatctcgaa
     1381 atggaacgga tgcgcgcgtt gtgggaacgc acggcggcga acggtatcga gcgcgagtgg
     1441 ttaaacgccg aggaactgcg cgagcgcgaa ccgaatatca ccgggctcgg cggcattttt
     1501 gtgccgtcca gcggcattgt cagctatcgc gaagtcacag cggcaatggc aaaaatcttc
     1561 caggccagag gcggtgagat tatttataac gccgaagtca gcgccctcag tgagcataaa
     1621 aacggcgtgg tcgtccgaac ccgtcagggc ggcgaatatg aagcatcgac gctgattagc
     1681 tgttccgggc tgatggctaa ccggctggtg aaaatgctcg gcctcgaacc gggttttatt
     1741 atctgcccgt tccgtggcga gtatttccgc cttgcgccgg agcataacca gattgttaac
     1801 catctgattt accccatccc tgacccggca atgccgtttt tgggcgtgca tctcacccgc
     1861 atgatcgacg gcagcgtgac cgtcgggcca aacgcggtgc tggcttttaa acgcgaaggc
     1921 tatcgcaagc gcgacttctc atttaacgac acgctggaaa ttttaggctc gtcggggatt
     1981 cgccgggtgc tgcaaaacca tctacgctca ggactgggcg agatgaaaaa ctcgctgtgc
     2041 aaaagcggct atctgcggct ggtgcaaaag tattgtcccc ggctttcgtt aagcgatctc
     2101 cagccctggc ccgccggtgt gcgggcgcag gcggtatcgc cggacggcaa gctgattgac
     2161 gattttctgt ttgtcaccac cccgcgcacg atccacacct gcaatgcgcc ctccccggca
     2221 gcgacatcag caattcctat tggtgcgcat attgtcagca aggtacaaac gctgttggca
     2281 agccagagta accccggacg cacgctgcga gcgacacgta gtgtggatgc cttacacgcc
     2341 gcatttaatc aataaccttt gaaaacagga cgtaacaatg aaacttaacg acagtaactt
     2401 attccgccag caggcgttga ttaacgggga atggctggac gccaacaatg gcgggatcat
     2461 cgacgtcacc aatccggcga acggcgacaa gctgggtagc gtgccgaaaa tgggcgctga
     2521 tgaaacccgc gccgctatcg acgccgccaa ccgcgccctg cccgccaggc gtgcgcttac
     2581 cgccaaagaa cgcgccaaca ttctgcgcaa ctggttcaat ttgatgatgg agcatcagga
     2641 cgatttagcg cgcctgatga ccctcgaaca gggcaaacca ctggccgaag cgaaaggcga
     2701 aatcagctac gctgcctctt ttattgagtg gtttgccgaa gaaggcaaac gtatttatgg
     2761 cgataccatt cccggtcatc aggccgataa gcgcctgatt gttatcaagc agccgattgg
     2821 cgttaccgca gctatcacgc cgtggaactt cccggcggcg atgattaccc gtaaagccgg
     2881 tccggcgctg gcggcaggct gcaccatggt gctgaagccc gccagtcaga caccgttctc
     2941 tgcgctggcg ctggcggagc tggcgattcg cgcgggcatt gcggctggcg tgtttaacgt
     3001 ggtcaccggt tcggcaggcg cggtcggtaa tgaactgacc agcaacccgc tggtacgcaa
     3061 actgtcgttt accggttcga ccgaaattgg ccgacagtta atggaacagt gcgcgaaaga
     3121 catcaaaaaa gtgtcgctgg agctgggcgg caacgcgccg tttatcgtct ttgacgatgc
     3181 cgatctcgat aaagccgtgg aaggcgcgct ggcctcgaaa ttccgcaacg ccgggcaaac
     3241 ctgcgtctgc gccaaccgtt tggcgtgtat gaccgctttg ccgaaaaatt gcagcaggcg
     3301 gtgagcaaac tgcacatcgg cgacgggctg gataaaggcg tcaccatcgg gccgctgatc
     3361 gatgaaaaag cggtagcaaa agtggaagag catattgccg atgcgctgga gaaaggtgcg
     3421 cgtgtggttt gcggcgctaa agcggatgaa cgcggcggca acttcttcca gccgaccatt
     3481 ctggtggacg tgccggccaa cgccaaagtg tcgaaagaag agacgttcgg ccccctcgcc
     3541 ccgctgttcc gctttaaaga tgaagccgat gtgattgcgc aagccaatga caccgagttt
     3601 ggccttgccg cctatttcta cgcccgtgat ttaagccgcg tcttccgcgt gggcgaagcg
     3661 ctggagtacg gcatcgtcgg catcaatacc ggcattattt ccaatgaagt ggccccgttc
     3721 ggcggcatca aagcctcggg tctgggtcgt gaaggttcga agtatggcat cgaagattac
     3781 ttagaaatca aatatatgtg catcggtctt taactggaga atgcgaatga acagcaataa
     3841 agagttaatg cagcgccgca gtcaggcgat tccccgtggc gttgggcaaa ttcacccgat
     3901 tttcgctgac cgcgcggaaa actgccgggt gtgggacgtt gaaggccgtg agtatcttga
     3961 tttcgcgggc gggattgcgg tgctcaatac cgggcacctg catccgaagg tggtggccgc
     4021 ggtggaagcg cagttgaaaa aactgtcgca cacctgcttc caagtgctgg cttacgagcc
     4081 gtatctggag ctgtgcgaga ttatgaatca gaaggtgccg ggcgatttcg ccaagaaaac
     4141 gctgctggtt acgaccggtt ccgaagcggt ggaaaacgcg gtaaaaatcg cccgcgccgc
     4201 caccaaacgt agcggcacca tcgcttttag cggcgcgtat cacgggcgca cgcattacac
     4261 gctggcgctg accggcaagg tgaatccgta ctctgcgggc atggggctga tgccgggtca
     4321 tgtttatcgc gcgctttatc cttgcccgct gcacggcata agcgaggatg acgctatcgc
     4381 cagcatccac cggatcttca aaaatgatgc cgcgccggaa gatatcgccg ccatcgtgat
     4441 tgagccggtt cagggcgaag gcggtttcta cgccgcgtcg ccagccttta tgcagcgttt
     4501 acgcgctctg tgtgacgagc acgggatcat gctgattgcc gatgaagtgc agagcggcgc
     4561 ggggcgtacc ggcacgctgt ttgcggtgga gcagatgggc gtggcgccgg atcttaccac
     4621 ctttgcgaaa tcgatcgctg gcgggcgtca ccgggcgcgc ggaagtgatg gacgctgttg
     4681 caccgggcgg tctgggcggc acctatgccg gtaacccgat tgcctgcgtg gctgcgctgg
     4741 aagtgttgaa ggtgttcgag caggaaaatc tgctgcaaaa agccaacgat ctggggcaga
     4801 agctaaaaga cggattgttg gcgatagccg aaaaacaccc ggagatcggc gacgtacgcg
     4861 ggctgggagc gatgattgcc atcgagctgt ttgaagatgg cgatcacagc aaaccggacg
     4921 caaaactcac cgccgggatc gtggcacgcg cccgcgataa gggcctgatt cttctctcct
     4981 gcggcccgta ttacaacgtg ctgcgcatcc ttgtactgct caccattgaa gacgctcaga
     5041 tccgtcaggg tctggagatt atcggtcagt gttttgatga ggcgaagcag tagcaccgtc
     5101 cccatgcagg aggcgacgct gcgcgtcttg tccggcctac gagaatccag gtcggataag
     5161 gcgtttacgc cgcatccgac aatctgtacg tgaacaggaa taaatctatg tcggccggat
     5221 aatgcggagc cgctctccgg caaaaagaat caataacaat taaacgcgtg acccggcgcg
     5281 ggaaatgtcg gggcgctctc ccaagtgaca cactttcgag aggattcagg atggggcaat
     5341 catcgcaacc acatgagtta ggcggcgggc tgaaatcacg ccacgtcacc atgttgtcta
     5401 ttgccggtgt tatcggcgca agtctgtttg tcggttccag cgtcgccatc gccgaagcgg
     5461 gcccggcagt attactggcc tatctgttcg ccgggctact ggtggttatg attatgcgga
     5521 tgttggcgga aatggcggtt gccacgcccg ataccggttc gttttccacc tatgccgata
     5581 aagccattgg tcgctgggcg ggctatacca tcggctggtt gtactggtgg ttttgggtac
     5641 tggttatccc gctggaagcc aacatcgccg ccatgatcct gcactcgtgg gttccaggca
     5701 ttcccatctg gttattttcc ctcgtcatta cccttgcctt aactggcagt aatttattaa
     5761 gcgttaaaaa ctacggcgaa tttgagttct ggctggcgct gtgcaaagtc atcgctatcc
     5821 tggcctttat tttccttggt gcagtcgcaa ttagcggttt ttacccttat gccgaagtga
     5881 gcgggatctc aagattgtgg gatagcggcg gctttatgcc caacggcttc ggtgcggtat
     5941 taagcgcgat gttgatcacc atgttctcgt ttatgggcgc agaaattgtc accattgccg
     6001 ccgcggaatc cgacacgccg gaaaaacata ttgtccgcgc cactaactcg gttatctggc
     6061 gtatttctat cttctatttg tgctctattt ttgtcgtcgt ggcattaatt ccgtggaata
     6121 tgcccggact aaaagccgtc ggttcttatc gctcggtact ggaattgctc aatattcccc
     6181 atgcgaaatt aatcatggac tgtgtgatat tactttccgt aaccagctgc ctgaactcgg
     6241 cgctgtatac cgcttccagg atgctctact ccttaagccg tcgcggagat gcgcctgcgg
     6301 taatgggtaa aatcaaccgc agtaaaaccc cgtacgtggc ggtgttactc tccaccggcg
     6361 cggcattctt aacggtggtg gtgaactatt acgtgcctgt gaaggtattt aaatttctga
     6421 tcgacagctc cggcgctatc gccctgctgg tttatttagt catcgccgtt tcacagttgc
     6481 ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg cttgcgcatg tggctttatc
     6541 cctggctcac ctggctggtc atcggcttta ttacctttgt gttggtagtg atgctattcc
     6601 gcccggcgca acagttagaa gtgatctcca ccgacttatt agcaataggg attatctgta
     6661 ccgtgccaat tatggcggct ggaataagct gataatgtgg caaaaaacac ccgttcataa
     6721 tacgcgctga taataatcag gagtcacacc atgaccatta cgtctctgga tggctatcgc
     6781 tggctgaaga acgatattat tcgcggtaat tttcaaccgg atgaaaaatt acggatgagt
     6841 ttgctgacat cgcgttatgc acttggcgtt gggccgttgc gggaagcact ttctcaactg
     6901 gtggcggaac ggctggtcac ggtggtgaat caaaaagggt atcgggtggc gtctatgtca
     6961 gagcaggagc tgctcgatat tttcgatgcc cgcgccaata tggaagcgat gttagtgagt
     7021 ctggcgattg cccgcggtag cgatgagtgg gaggcagacg ttctcgcaaa agcgcatctg
     7081 ctcagtaagc tggaagcctg tgacgccagc gagaaaatgc ttgatgagtg ggatctgcgt
     7141 catcaggcgt ttcatacagc aattgtggcg ggctgtggtt ctcactattt gctgcaaatg
     7201 cgtgaacggt tgtttgatct ggcggcgcgc tatcgattta tctggctgcg gcgaacggtg
     7261 ctttcggtag aaatgctgga ggataaacac gatcagcacc agaccctgac tgcggcggta
     7321 ctggcgcgag ataccgcgcg cgccagtgag ttaatgcgcc agcatttact gacgccaatt
     7381 cccattatcc agcaggcgat ggctggcaat taactactct tccggaatac gcaacacttg
     7441 ccccggataa attttatccg ggctttttag catcggttta ttcgcttcga agattttatt
     7501 gtacagatta gcgttaccgt agacctgttt ggaaatggca ctcagagtgt cgccagactt
     7561 aacggtataa aactggctgg cagtggctgg tgtcgccgtt ttcacctgat catcaacact
     7621 ggcaataccg gaaatattcc ccaccgcaac aaggattttc tccttcgcct cctgactcag
     7681 gccgtcacca gtgaccgtcg ctttgccgtc ggcaatttga atgttcactt tatcggcatc
     7741 aggtataccg gttttgctca gatgctcctg caccttcttc gcctgatcgt ctttatcgtg
     7801 ctgacctgta accgcgtccc agagtttttc tccggcatct ttcacaaaat tgaacagacc
     7861 catagctacc tcattcgtta acggaaacac tcagaaagtg tagcagagga acgcggcgcg
     7921 ttaacgccgc gggggggagc acaggtttaa tcgcgggttt gcacccagaa cgcgtgaatc
     7981 aaaccgggga tatacccaag cagtgtcagc agaatattaa taatgaacgc ccaaccgaac
     8041 cctttaccga gcagcacgcc gagcggcggc agaatgatgg tgatgacgat tctccagaaa
     8101 cccatatgta ctccctataa gaaaattact cattgtttaa aaagagattt tatctcttaa
     8161 gcgtagtaat tttagcggag cctgccagtt tcagcttacg cttttactct catttacgct
     8221 gctgaggctg gtatatgttg catttttagt ttagagttta cttaatttag aaaatactta
     8281 aataaatatg actgaactcg cgcaattaca ggccagtgcc gaacaggcag cggccttatt
     8341 gaaagcaatg agccatccta aacgattgct gattctgtgc atgcttagcg gttcccccgg
     8401 caccagcgcg ggagagctga cgcgcattac cggactgagt gcctctgcga catcacagca
     8461 tctcgctcgt atgcgggacg aagggcttat cgacagccaa cgggatgccc aacgcattct
     8521 atattccatt aaaaatgagg cggtaaatgc cattatcgcc accctgaaaa atgtctattg
     8581 tccgtaagga gtcactatgg ctttgacaac catttcgccg catgatgcac aagaattaat
     8641 cgcacgcggc gcaaagttaa tcgatattcg tgacgctgat gaatatcttc gtgaacatat
     8701 tcctgaagca gatctggctc cattatccgt gctggaacag tcaggtcttc cacctaaatt
     8761 acgtcgcgag caaattatat tccactgcca ggcaggtaaa cgcaccagta ataacgccga
     8821 taaattagca gcgattgccg cccccgcaga aatattttta ctcgaagata ggattgatgg
     8881 ctggaaaaga gctggattgc cagtagcggt aaataaatct caacccttgc cgttaatgcg
     8941 ccaggtgcag atcgctgcgg gtggtttaat attaatcggc gttgtactgg gttataccgt
     9001 aaatagcggt ttcttcttat taagtggctt tgttggtgcc gggttactgt ttgcaggaat
     9061 cagcggtttt tgtggaatgg caaggttgtt agataagatg ccgtggaacc aacgagcttg
     9121 agaagcgacg ccggacgcgc cctagcagcg acatccggcc tcagtaatta gatcaggaaa
     9181 tcgtcgagag atttaccttc tgccagcgcc tgagcaattg gcttcggtgt acggccctga
     9241 ccggtccagg tcttagtttc accgttaaca tcagtgaatt tatatttcgc cggacgcggc
     9301 tggcgttttt taccagcacg tggtgcagca gcagagctat tacccaataa ctcttccggg
     9361 ttaattccgt cagctttcat cagctccagc caggtgctaa ttttttcctg acgctcagcc
     9421 agttcacgct gctgctgttc ttcttcttca cgtctttctt tagtgacaac cctgaatttt
     9481 tcgagcattt cttcaagaac gtcaatggag aattcgcgag ccatcgcacg gagggttcga
     9541 atgttattta aactttgtaa cattacggac ataataaaga aaacctttta acgccaaaac
     9601 aaaaaagtat ttcgtcgcgt agtgtaagcg atcacttatt atatttccac aaaatttaat
     9661 ctatttacag tcctgcgcga gattatttca gctgttattt tctgaaactc tcagaataat
     9721 ttcaagatta tatattagcc attttgatga gtaatttagg ttattggtga acagagtggc
     9781 gctctctgct cgctaactcc gcaagcctga taaaaccgaa gtgaataaaa atgcaatata
     9841 acagaataaa agaaaacacc cctcactggt gctttctgct gttaatgtac attttctggc
     9901 atcaaaaatg ctgtaaatga cccggtcacg acaaacaccg aattctctac gcttatagcg
     9961 ttacctcacc cccaaacttt cattagtgaa attaaaggca aggtcatatc caattgattt
    10021 aattgaaaat aaatcatatc ggcatgtaaa aatccacaat gtacaaaaaa cgagctgtta
    10081 cggaatattt tatctacaaa aaactgacta aataaaagtt tttcactaat tgattagtca
    10141 tagccagcga tatacgctat gcgaaaatgc agatggcaat gagatccact gctttcatct
    10201 ccattaacat cccattacgc tttattaagg agcattagca tgttctcacc gcagtcacgc
    10261 ttgcgtcatg cagttgcaga tacgttcgcg atggttgttt actgttctgt cgtgaacatg
    10321 tgtattgaag ttttcctctc cggaatgagc ttcgaacagt ctttttattc cagattggta
    10381 gcgattccgg tgaacatctt aattgcatgg ccatacggta tgtaccgcga tctgtttatg
    10441 cgcgcggcac gcaaagttag cccgtcgggc tggataaaaa atctggctga tatcctggct
    10501 tatgtgacgt tccagtcacc ggtgtatgtg gcgatcttgt tagtggtggg cgcagactgg
    10561 catcagatta tggcggcggt cagttcaaac atcgttgttt cgatgttgat gggggcggtt
    10621 tatggctact tcctcgatta ttgccgccga ctgtttaaag tcagccgtta ccagcaggta
    10681 aaagcctgac tcttcttttc gcgactggca tcgccagtcg cgtctttaac tggcttcgcc
    10741 aaataaacca ttcaaataac gttcaagcgc aacacgcgaa ctgaagccat gcgggatacc
    10801 gtaatgctcg tgtcgctcgc ctgctaaata gagcggaaat tgctggtaat gatcgcaagt
    10861 tttaatatcg gaagcgggtt cagccagtgt tgaactgcgt tcttttaatg ttggatgaat
    10921 taccagagcg gtacgtccca ttcgcgcttc gcgattaaca tagacataat tttccccacg
    10981 gcggtaacca tacgctttct gggttaccac atcgacagta aaaccgactt tttcaagtac
    11041 gcgcgccacc tcgtctggtc gtaaatacat atttgatcct cgttattttc aaccgcg
//
LOCUS       DAJTOR010000134        11097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTOR010000134 DAJTOR010000000
VERSION     DAJTOR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.134"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..668
                     /locus_tag="OO249_003113"
     CDS             <1..668
                     /locus_tag="OO249_003113"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5637322.1"
                     /translation="GAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLA
                     PMAKALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKA
                     IGLEIPELSGKLKGHAQRVPVKTGSVTELVSILGKKATAEEVNNALKQATTNNESFGY
                     TDEEIVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKF
                     AKL"
     gene            complement(710..2150)
                     /gene="aldA"
                     /locus_tag="OO249_003114"
                     /pseudo
     CDS             complement(710..2150)
                     /gene="aldA"
                     /locus_tag="OO249_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(2305..3147)
                     /locus_tag="OO249_003115"
     CDS             complement(2305..3147)
                     /locus_tag="OO249_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5637323.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLRQKLPVCEPLV
                     CKRA"
     gene            complement(3419..7321)
                     /gene="hrpA"
                     /locus_tag="OO249_003116"
     CDS             complement(3419..7321)
                     /gene="hrpA"
                     /locus_tag="OO249_003116"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5637324.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            7522..8127
                     /gene="azoR"
                     /locus_tag="OO249_003117"
     CDS             7522..8127
                     /gene="azoR"
                     /locus_tag="OO249_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5637325.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(8178..9494)
                     /locus_tag="OO249_003118"
     CDS             complement(8178..9494)
                     /locus_tag="OO249_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR5637326.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWPFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWPVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(9484..11097)
                     /locus_tag="OO249_003119"
                     /pseudo
     CDS             complement(9484..11097)
                     /locus_tag="OO249_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT         2751 a         3024 c         2649 g         2673 t
ORIGIN      
        1 ctggtgcgaa gaaggtgttg atttccgccc ctgccggtga aatgaaaact atcgtttata
       61 acgtcaatga cgacactctg gatggcaacg acaccattgt ttccgtggcg tcatgcacca
      121 ctaactgtct tgcgccgatg gccaaagcct tgcatgacag tttcgggata gaagtcggca
      181 cgatgacgac cattcatgcc tatactggca cccagtcact ggtggatggc ccacgtggta
      241 aagatttacg tgcttcacgc gcagcggcag aaaatatcat tccccacact acgggggcgg
      301 caaaagccat tggtctggag atcccggaac tgagcggcaa actgaaaggt catgcgcaac
      361 gcgtgccggt gaaaacaggt tcggtcactg aactggtatc gattctcgga aaaaaagcga
      421 ctgccgaaga ggtgaataac gcacttaaac aagcaaccac caataacgag tcatttggtt
      481 ataccgatga agaaatagtc tcttccgata tcattggcag ccatttcggt tcggtgtttg
      541 atgccacgca aacggaaatt accgccgtgg gcgatttaca actggtgaaa acggtcgcct
      601 ggtacgataa cgaatatggc ttcgtcacgc agcttattcg caccctcgaa aaattcgcta
      661 aactctgacg cgcacaggcg gaggaaaaaa cctccgcctc ttttactcat tacgactgta
      721 aataaaccac ctgggtctgc agatattcat gcaggccatg tttaccatct gcgccgccaa
      781 taccggattt acgccatccg gcgtggaagc cttgcatagc ttcgaagttt tcacggttga
      841 tgtaagtttc accaaacttc agccctttaa tggctttcat cgcgacgttc agattttggg
      901 tatagatttg atgaggtcag gccgtaatca ctgtcattag ccattgagat agcctcttcc
      961 agcgtgtcaa atgcgaccac cggcagaacc gggccaaagg tttcctcatg cataatcgac
     1021 atttcctggc gaacatccag cagcaatgtc ggcggataat aatatccttt cccctctacc
     1081 gctttgccac ccaacgccac tctcgcccct tcttctactg cgcgcgccac tttttgctca
     1141 accctttcca acgccgcggc gttaatcaac ggccccatcg caatgtcgtt gcgttcagcg
     1201 gggttaccaa attgaaccgc ctgcatcgct tcacccagcc gattgacgaa ctgatcataa
     1261 atgcctttct gtacataaac acgttctgca cagttacaca cttgcccact attaatgacg
     1321 cgtgaatcaa cgatggcttt gactgccagt tcaagatcgg catcgtccat tacgatagct
     1381 ggtgctttac cccccaattc cagacacact ttggtgatgt ttttcgccgc agtcgccatg
     1441 atcttctcac ctgcagagac gctgcctgtc atactgacca ttgcgacctt tgggttaccc
     1501 gccagttctt gcccaacggt ttcaccacgc cccagtacaa ggttaaacac gccgcgcgga
     1561 aggcctattt catcgacgat tttggcgaat gcaatcgcat tgtttggcgt aaattcacta
     1621 ggtttaatga cgatggtatt accggtcaaa agagcgggag ccattttgcg ggcaatgagg
     1681 aagaacggga agttccacgg cagaatgccg gtagtcacac caagcgcacg tttaaacaaa
     1741 agaatatttt ctcctggacg atcgctttga ataatctcgc cctcgtaacg ccgtgcccac
     1801 tccgccatgt aatcgatata gtcggcagta aaagccactt cgacttcagc cagctgctgg
     1861 atcttgcccc cttcttcaac aatcagcgca ctgatttcac tggcgcgttc gcggatcccg
     1921 gcggagattt tgcgcaacca actggcgcgt tcaatagcag gcaacgcttc ccattctggt
     1981 tgtgcacgtt ctgctgcatc gattgcctta cgggcatcct cggcctgacc atcgggtatg
     2041 cgggaaatga cagcctctgt agcagggttt accacatcaa tccatgcgtc tccacgccag
     2101 gtaacaaact gtccatcgat atacatagga tgttgaacgg gtactgacat gggcgactcc
     2161 tgtgatttat atgtttgttc ttggtaaatt cattgttaat taacatcaat catcatagag
     2221 acggcatcgt gaaaggacaa ttctgtgaag ggcttcataa aaacaagtgt aaaaggaata
     2281 aaaggcagaa ataaaagtca ggagtcatgc ccgtttgcaa acaagtggtt cacaaacggg
     2341 caatttttga cgtaacgagc gactttccat cgcctcgatg agcaccgcat cgtgtttaag
     2401 cgtttgccat gcatggatga cttctgccgg aaaatcaacg tgaacgataa aatctcgccc
     2461 gcggggaccg tagccatcgc tatcatcgcg taaacgcggc agctcgccag tgagtagtga
     2521 gagataacgc tcaaccggcc ataatccttg taactgatta ataaagatta cactgtctgc
     2581 gttatttcct aactgaggaa cgaatccggg ataacttaac cagcgtggtg catcgggatt
     2641 gtccccagtc atacggcgga acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac
     2701 aacgatagcc gtatgaactc gttctacggc ctgattaaat agcgcgatgc taaagcgtgc
     2761 gttttcaccg cagtttgttg actggtcttc aatccagatt ttttcatgcg gaatgtgcca
     2821 gaactgatga gcgatatccg ccaggatggt cgcttctgct ctgccagtgg tgcggatagt
     2881 gttgtagtgc ggatgctgtg cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc
     2941 accactaatc agtaaaggaa tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt
     3001 cggcataacc gcattgcctg caaggatcac gcaatcggcc tgatacggca cctcaccgga
     3061 gaaatcatcc tgcgccagcc actgtccgat aacatttatg gcatctatag ttgccggcga
     3121 aagcgtcgga aacggggtaa tgttcatggt tcctccttat cgttccctta agaatactgc
     3181 gtgagataat agtgagaagc tgagcactct gaaaggagga agtcgtatgt ccttaaccgc
     3241 cactccgcaa gcctgacata tcttttaagg aaatgctgta gataaacatt attcatcttg
     3301 cagcagagaa ttgtattttt ctgaattcct gaatttttac ccttaccgtt ttagaattaa
     3361 taaaaaaccc ggcgctaaat gcttaccggg ttttagattt attaggcaaa tagcagggtt
     3421 aaccgctaat ctgctccatc gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac
     3481 caagttgttg agcgaagtaa ctaacgcgca gctcttctat catccaacgg atctctttca
     3541 cgtcttcatc ctcacgacgt gcaggcggca gtttgttgat ccattgctgc cacgcctgct
     3601 ggacgttttc gactttcagc atctgtgcac gatcgcgatg tggatcaacc gccagttttt
     3661 ccagtcgttt ttcaatcgcc tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt
     3721 taccagtgac aaaaccgcga tataccaacc cgcccatctg cgctttaatg tcagaaagcc
     3781 ccagtgccat ggtcatatcc acccgccctt tcagacgttt gttgatattg aacactgccg
     3841 taagaatttg ctcgacctgc ttcgcaatat ctaccaccgt gtcgttcagt tcggcacgca
     3901 ctttctcatg cagcgcagca aagccttctt ccgtccagac cgggccacca ttggcgtcga
     3961 tcagttgatc cacaccgcag gagatacagt cgtcgatcag ctccagcact ttgccatacg
     4021 ggttgaagta cagtcccagc ttggctttgt tcggtaactt ttcatgtaaa tacttgatcg
     4081 gcgagggaat atttagcagc agtagacggc gaagaccatt ccacattgcc tgtttttgct
     4141 ccagcgggtt atcaaacagt ttgatcgcca cgctgtcgcg ttcatccacc agcgccggcc
     4201 acgccttcac tttatagttg ccacgcttct gttcgtaact ttccggcagc tgaccaaaac
     4261 tccagatatg taagccgctc tgctcgatac cgtcatccgc caccgcggac agcgtttcct
     4321 gcactttgcc tttcagcgca tctttcagat cttgtagcga gcgcccttct tttagcttct
     4381 tgtttttgtc atccaccacg cggaaggtga ttttcaggtg atcgggcacc tgatcccagt
     4441 gccagtcttc gcggtcaacg gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt
     4501 cgagcaacgg taactccagc ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg
     4561 caggcacaaa attacggcgt accggtttcg gcaacgattt aatcagagca atcaccaatt
     4621 cgcggcgcag accggggatc tgccattcaa acccgctttc ctcaacctgg ttaagtaacg
     4681 gcagcggaat atgtacggtc acaccgtccg cgtctgcgcc tggctcaaac tgatagctca
     4741 aacgcagctt gagattgccc tgatgccaga agttcgggta atccagcttg ctgatttttt
     4801 ccgccccttc tttgatcaac atgctctttt caaagttgag caaatcaggc gtttcgcggc
     4861 tgactttttt ccaccagcta tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct
     4921 ggtcgtagaa ctcaaacagc gtttcgtcat caaccagaat atcgcggcga cgtgatttgt
     4981 gttccagctc ttccacttcg gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg
     5041 tctgccagtc accttccacc agcgcatggc gaataaagag ttcacgacat aacgccggat
     5101 cgatctggct gtagttgacc ttgcgcgcgg caacaatcgg caaaccataa acagtgactt
     5161 tttccgttgc catcaccgcg ccctgcgccc gttcccagtg cggttcgctg taggtacgtt
     5221 taatcaaatg ctgagcgaca ggctccaccc attccgggtc gatacgcgca gcaatgcgcc
     5281 cccacaggcg gctggtttct accagttccg ccaccattac ccatttcggc ggttttttga
     5341 ataaaccaga accggggaag atggagaaac gcgcgttacg tgcgccggta tattcttgtt
     5401 tatcggcatc tttcatgccg atatgggaaa gtaaacccgt cagcaacgca atgtgaattt
     5461 cgcgatactc cgccggttcg ctgttaaccg gaatgccaag ttctttcacc acctgacgca
     5521 actgggtgta gatatcctgc cattcgcgca cgcgcagata gttgagataa tcggtacgac
     5581 acaggcgacg gaaggcgttt gaagaaagcg ccttttgctg ctcgccaaga taattccaca
     5641 gatttacaaa cgcgaggaag tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg
     5701 atgcctgctg tttgtccatc ggccgctcac gcgggtcctg aatggagagc gcggacgtga
     5761 taatcatcgc ctcacgcacg cagccatgtt tctgcgcttc cagcaccata cgcgccagac
     5821 gaggatcgac cggcagttgc gagagctggc gaccgagcgg cgtcagttta taggcgctgg
     5881 cctgttcatc cgtggtgatc gcgcccagtt cttcgagcag acgcacgcca tcctggatat
     5941 tgcgtttatc cggtgcttcg acaaacggga acgcagcgat atcgcccagc cccagcgcgg
     6001 tcatctgcaa aataaccgag gccaggttgg tacgcagaat ctccggatcg gtaaactccg
     6061 ggcgcgagag gaaatcgtct tcggaataga gacgaataca gatcccttcg gacacacgac
     6121 cacaacggcc tttacgctga ttggcagacg cctgtgaaat gggttcaatc ggcaaacgct
     6181 gcactttggt gcgatagctg tagcggctga tacgcgccgt accggggtcg ataacgtatt
     6241 taatccccgg tacagtcagc gaggtttccg cgacgttggt cgccagcaca atgcgtcgcc
     6301 cgctgtgcga ctggaacacg cggttctgtt cgctattcga aagccgcgca taaagcggca
     6361 agatttcggt atggcgtaag ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc
     6421 gctcgccgct cataaagatc agaatgtcgc cagggctttc ctggctcagt tcgtctacgg
     6481 cgtcaaaaat cgcctgcaac tgatcgcgct cggtgtcatc ggcttcttca acaatcgggc
     6541 gatagcgcac ttccaccgga taggtccgac cggagacttc gataatcggc gcattattaa
     6601 agtggcgaga aaagcgttcc gggtcgatag tcgcggaagt gataatgatt tttaggtcag
     6661 gacgccgcgg cagcaactct ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt
     6721 cgtgtgcttc gtcaataatg atggtgtcgt actgcatcag caggcggtct tgctggatct
     6781 ccgccagcag gataccgtcg gtcatcagct tgaccatcgt gttatcactt acgtgatcgc
     6841 tgaaacgcac tttgtaaccg atgcaaccgc ctggctccgt tttcagctct tccgcaatac
     6901 ggttcgccac cgttcttgcc gccagacgac gcggctgggt atggccgatc agtcctttaa
     6961 tcccgcgccc cagctccata cagattttcg gtagctgggt cgttttaccg gaaccggttt
     7021 ccccggcgac gatcaccacc tggtgatcac gaatcgcttc gagaatatcc tgttttttct
     7081 gactaactgg caaattgtcc ggataagtga tttccggtcg tgccgcttca cgcagcagga
     7141 ctttccctgc cgcctggtca atctctttcg ccatctcctg gaaaatggcc tgttgtgcat
     7201 caggattttt aaccttctta acaccatgca gacggcgaga aaaacgcagt ctgtcgcgca
     7261 gcatcaacga atccagccgc tgctgcaaag cagtaaaggt caatttttgt tgttctgtca
     7321 taacgttagt gggcagtagt ctgcctgatt aattttcttt taaatgatag atatagagta
     7381 ccacatcgcg gctttctatg cgttgttcaa taaattcgaa cataggcttc gatttattgc
     7441 gctatctctg tggcaggatt gtgaataaag tgtcagcaag caacggggca tcgccaattc
     7501 aaacatctat aaggaaacac catgagcaag gtattagttc ttaaatccag catcctggca
     7561 gggtactctc agtctaatca gttgtccgat tattttgttg aacaatggcg cgaaaagcac
     7621 tccgctgatg aaatcaccgt tcgcgacctg gctgcaaatc cgattccggt actggatggc
     7681 gaactggttg gcgctctgcg tccgagcgat gcgccgctga ctccgcgtca gcaggaagct
     7741 ctggcgcttt ccgatgagct gattgccgag ctgaaagccc acgacgttat cgttattgcg
     7801 gcaccgatgt ataacttcaa catctcaact cagttgaaaa actattttga cctggttgca
     7861 cgcgcaggcg ttactttccg ctataccgag aacggtccgg aaggtctggt aacgggtaaa
     7921 aaagccatcg ttattaccag ccgcggtggg atccacaaag atggaccaac ggacctggtg
     7981 acgccgtatc tgtccacgtt cctcggcttt atcggcatta ccgatgtgaa atttgtcttc
     8041 gccgaaggga tcgcatacgg tccggaaatg gcagcgaaag cgcagtctga cgcgaaagcg
     8101 gccatcgaca gcattgttgc tgcataaccg gacacgatct tgatgtggac tgtagttcag
     8161 tccacatctc aatccactta cctgttttcc cataatttca gcatcgcttt gtgatcttcc
     8221 ttaagcacaa tatgcgaacg tctggctcga atataactaa tcgcttcatc aacggtttta
     8281 cagtgtccgt aacataacaa ccatgccgcc actaccagcg cactgcgcga taatcccaac
     8341 gcgcaatgga ccagaacgct gccttgctct tcgcgtaatg tttccagcat cgccacggcc
     8401 tgtcggagct ccccctcttc cggaaccacc agatccagca tcggtacaca gaaatagagt
     8461 cgatcttttg tcgctcgtcc ccgagggaat tcaaaggtga cgtccagaac cgcattctgt
     8521 gccggaatat gtcgtggaaa cgcccctaaa taaacaccag cagtaatttt gctcaccggc
     8581 tccaggcgac gacaaaacca acgcatagac agccacatcc cgatgcgcca gggcaatgtc
     8641 agccagtaaa cggcgggcgg gagtttcccc tgactatctt tccctgttgt tatcgcgcca
     8701 agcccaccgt acccacggcc aataatgagt agcgataata ctggccaaca taaccagact
     8761 ggccaccata actgaatcat caccattagc tccatcaaca caatgcacga gcccgcgcct
     8821 acgacatatg gcagtgctat tttgattcga cgttgatcag gtttctgata attccaacga
     8881 cgatcgacgg gcaccatcca gtcaatcaac atacctaccg ccagccctgt gatgacatca
     8941 ataaaatgat gctgccaggt cgtcagagta gaaatagcga tgagtaaaaa ccatccgcca
     9001 cagactttac gccacctctc agccagatgc tgacgaaagt gacgccaaag tagccagcag
     9061 agaataatat gcagcgaagg agactggtta taaggcagat caaacagttc aagttgcgaa
     9121 aatggccatc ccgtcacccc actcacttca ggacggataa aactaaactt cagcggatag
     9181 agcaaaaaac cgcagcaggc cattaccgtt gccagaataa gccggtggac aagtcggcgc
     9241 tgttcgaatg tcgtgctaca aacgaacagc gaaaatccat ataaaagatc cagactccag
     9301 taaggaacaa tagtccaggg aagaaaaggg atcgccgttt cccaaccgaa tacctgactg
     9361 gggatatcat ggctgttaag gtcctgaacc gcggtgaact gattaagaga tccataggtg
     9421 aagaaaaaaa acggggccag caacaataac cagccagcgc cttgtagcaa tacgttccga
     9481 cgttcagtta tcacgacgca ccgccatcga aaccgtaaaa ataccccact catcaatccg
     9541 ttgtgtgcat ttatcaaatc cggcatcacg cacgagtgaa tccatctccc cttgcgaacg
     9601 cacgcgcatt acccacggtt taccatcttt atgactggtt aacaccccgg caatcatctc
     9661 cagttgaggg tgccacggct gcccggtgta gatgagaatg ccgaccggtt cgatggcatt
     9721 ggcaagacct gcgagtgagt tttttacctg ctcattttcg ggaaaaagct catacagtcc
     9781 agagacaatt gccagcgtag gccgcggagt taacgcgctg agttcctccg ggttaaacgc
     9841 atcgccctgt tcaaaacgca cccgcccaga cattccccgt tgggcaatca tctcttgccc
     9901 ctgtgcaaca tttaactcgc tgtaatcacg taacaaaata tcgcttacgg caggctcgtt
     9961 tgccagcgca tccagtacat agcgcccatg ccctgcggca atgtcaacca cgcggacggc
    10021 taaacctttg gcgtggagat cggcaacggc ttgtttaatc agtatttgta aatgggtttt
    10081 gcgctggcga ataccgcgcc agccaacact gttcaggtag attttgtcga ttaagcgccc
    10141 gaatgcgtta ctaccttgcg gttgattttg atagacataa tccagcgaac tgcctgaatc
    10201 aaagccggtg ctcattccga gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc
    10261 ctttcgcata aagcgatacg ctaaatcaac cggcgataat agcacgggtc caccagaaag
    10321 tagccgccat tgatccgctg atggtcctgt gcagtcttca tgctgataat caaatttttg
    10381 cgatttgtta gcatataaac gactaataaa gctttgcatt ttttcaaatg ccagcgccct
    10441 gttctcttca cccaacgtgt cgtgataaaa gcctggcagc agatgcagct ctttcagagg
    10501 gctacgtaat ctctgataaa aatcaatctg cggttggcga tgcaccacat agtcatcgcc
    10561 tgatatcaga agttgcgtgg ggagcgtaat cgccgccgca tctctaataa tacgttcaga
    10621 cgttttatag agatcgagca agatattgac ggcaatcgcc cgtgtgatca gcggatcatt
    10681 attgaaactc gccacccgtt gccgatcgtg ggtcaaatag cgtcctttca cataggaatt
    10741 aataaaaaac agaccacgca gacgatgcca taacgccagc gcaggacgtg ccagcggcac
    10801 atacaattta accttaaagg ccggagaagc cagcaccagc ccgcgaattg caggcgcata
    10861 atcatgaacc tatgtggcaa ccagcactgc gccgacgctt tgcgcgatca caaccacctc
    10921 ttccagtcca acctagctgt cgctggcagc aaaacggaca aattcatcga catcctgcac
    10981 tgaacgcgca agagatgggc tataaccacg cggccccgaa gtttttccat gcccccgagc
    11041 atcccatgca taaaaagcag tatctggcat cgccagttca tcaacgatat gttgtag
//
LOCUS       DAJTOR010000135        10992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTOR010000135 DAJTOR010000000
VERSION     DAJTOR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.135"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(169..375)
                     /locus_tag="OO249_003120"
     CDS             complement(169..375)
                     /locus_tag="OO249_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5637327.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            687..776
                     /gene="kdpF"
                     /locus_tag="OO249_003121"
     CDS             687..776
                     /gene="kdpF"
                     /locus_tag="OO249_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5637328.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            776..2449
                     /gene="kdpA"
                     /locus_tag="OO249_003122"
     CDS             776..2449
                     /gene="kdpA"
                     /locus_tag="OO249_003122"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5637329.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALSADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2472..4520
                     /gene="kdpB"
                     /locus_tag="OO249_003123"
     CDS             2472..4520
                     /gene="kdpB"
                     /locus_tag="OO249_003123"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR5637330.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADIAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSITNDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4529..5072
                     /gene="kdpC"
                     /locus_tag="OO249_003124"
                     /pseudo
     CDS             4529..5072
                     /gene="kdpC"
                     /locus_tag="OO249_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            5065..7749
                     /gene="kdpD"
                     /locus_tag="OO249_003125"
     CDS             5065..7749
                     /gene="kdpD"
                     /locus_tag="OO249_003125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR5637331.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTDAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNALGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7746..8423
                     /gene="kdpE"
                     /locus_tag="OO249_003126"
     CDS             7746..8423
                     /gene="kdpE"
                     /locus_tag="OO249_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR5637332.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8639..8896)
                     /locus_tag="OO249_003127"
     CDS             complement(8639..8896)
                     /locus_tag="OO249_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637333.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            9019..>10992
                     /locus_tag="OO249_003128"
     CDS             9019..>10992
                     /locus_tag="OO249_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5637334.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRV
                     SMNPQEANYAYSRGEVELVRLPDAEGRIAAEGAL"
BASE COUNT         2466 a         2802 c         3004 g         2720 t
ORIGIN      
        1 aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatattt ttcctgaata
       61 tatttcagct gggtaatacc atatctaaat atttacctga acatcttcct aataaaaaaa
      121 ccgccagcat ctccactagc ggttttcaat gtattcaaaa ctcggcaatc agtaaaaatc
      181 accggttgcc ttttcggcct gttccatcca aaccggttta tcgctggttt tcgaccagac
      241 gcggtgcagg tagctgtaaa agcgggcgcg gtctttccag aaaagcatga aaggcagcgc
      301 cagaacgccc gctgcaaccg catatgtacg gcgtaaaaag ataagccatg caggatattc
      361 tctgtagagt tccatacttt tctccccctt ttgtatctac ccggtgaata gcaccggaaa
      421 aatgaatttg tttatctgat gaaaatagta ccgccttttg tgtaatttta ctactcatcc
      481 gaccacttat ttttgcttat tgatggttta tttacattca ctttgtaatt aagttgcaca
      541 aaagttaaat taatgctaaa cattagttaa accatggctt ttgccatttt tatacttttt
      601 tacaccccgc ccgcagattt ttgcgaaatc tttgcagcca gaattctact cttccggtat
      661 cacttttagg ccactggagg tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg
      721 tgtttttatt actgggttat ttggtttatg ccctgatcaa tgcggaggcg ttctgatggc
      781 tgcgcaaggg ttcttactga ttgccacgtt tttactggta ttaatggtgc tggcgcgtcc
      841 tttaggcagc gggctggcgc ggctgattaa tgacattcct cttccaggta cagcgggcgt
      901 tgaacgtata ctctttcgtt tacctggcgt ctctgaccat gagatgaact ggaagcaata
      961 tctttgtgcc attctcggcc tgaacatgct ggggctggcg gtgctgtttt ttatgttgct
     1021 cggtcagcac tatctgccgc ttaatccaca gcagttgccg gggctgtcgt gggatctggc
     1081 gctgaatacc gccgtcagct ttgtcacgaa taccaactgg caatcttata gcggtgaaac
     1141 cacgttgagc tatttcagcc agatggcagg cttaacggta caaaactttc tttctgccgc
     1201 cagcgggatt gcggtgattt ttgccttcat ccgtgcgttt actcgccaga gcatgagcac
     1261 actcgggaat gcctgggtcg atctgctacg catcacgtta tgggtgctag tccctgtggc
     1321 gttgttgatc gcactgtttt ttattcaaca aggtgcgctg caaaactttc tgccctatca
     1381 ggcagtgaat accgttgaag gagcgcaaca gctgttaccc atgggaccag tagcttctca
     1441 ggaagcgatc aagatgctcg gtactaacgg cggtggcttc tttaatgcca actcgtcgca
     1501 tccgtttgaa aacccaaccg cactgaccaa cttcgtgcag atgctggcga tcttcttgat
     1561 cccaacagct ctgtgctttg cctttggtga agtgacgggc gatcgccgcc aggggcgcat
     1621 gttgctgtgg gcgatgtcag tgatttttgt catctgcgta ggcgtggtta tgtgggcaga
     1681 agttcagggt aatcctcatc tgctggcgct cagcgcagac agcagcatca atatggaagg
     1741 taaagagagc cgtttcggcg tgctggtcag tagcctgttt gcggtcgtga ctacggcggc
     1801 gtcctgtggc gcggtgattg cgatgcatga ttcgtttacc gctctcggcg gcatggtgcc
     1861 gatgtggcta atgcaaattg gtgaagtggt gttcggcggt gtcggttctg gtctttacgg
     1921 catgatgctg tttgtcctgc tggcggtgtt tattgccggg ctgatgattg gtcgtacacc
     1981 ggaatatctg ggtaaaaaaa tcgacgtacg cgagatgaaa ctgactgcac tggcgattct
     2041 ggttactcca acactggtac tgatgggcgc ggcgttggcg atgatgaccg acgccggacg
     2101 tagcgccatg cttaaccctg gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcgtc
     2161 cgccgctaac aacaacggca gcgcctttgc cggattaagc gccaactctc cgttctggaa
     2221 ctgtttactg gcgttctgca tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc
     2281 aattgccggt tcgctggtga gtaaaaagag ccaacccgcc agctccggca cgctgccaac
     2341 gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg ttgctggttg gcgcactgac
     2401 ctttatccct gccctggcgc ttggtccggt ggcggaatat ctctcctgat gatattgagt
     2461 gagcactgaa tatgagtcgt aaacaactgg cgctattcga accaacactt gtcgttcagg
     2521 cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt
     2581 ttatcgtctg gatcggcagt ctgctgacca cctgtattag catcgcgatg gcaagcggtg
     2641 cgatgcctgg caatgcgctg tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac
     2701 tgttcgctaa tttcgccgag gcgctggcag aaggccgcag taaagcgcag gccaacagtc
     2761 ttaaaggggt gaaaaaaact gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg
     2821 cggcggacaa agttcctgcc gatcaactgc gtaaaggtga catcgtgctg gtagaagctg
     2881 gcgatattat cccctgcgat ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg
     2941 ccatcaccgg ggaatcggca ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca
     3001 ccggcggcac gcgtattctt tctgactggc tggtgattga gtgtagcgtt aaccccggtg
     3061 agacatttct ggatcggatg atcgcgatgg tggaaggcgc acagcgacgc aaaacaccga
     3121 acgagattgc cctgactatc ctgctgattg ctctgaccat cgtcttttta ctggcaaccg
     3181 ccacgctgtg gccgttttcc gcgtggggcg gtaatgccgt gagtgtaacg gtactggtgg
     3241 cgctgctggt ctgtctgatc ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg
     3301 ccgggatgag tcggatgctg ggcgcgaatg tgattgccac cagcggacgt gcagttgaag
     3361 cggcaggtga cgttgacgtt ctgctactgg ataaaaccgg caccatcaca ctcggtaacc
     3421 gtcaggcgtc ggagtttatc cctgcgcagg gcgtggatga aaaaacgctg gctgacgccg
     3481 cacaactggc ttcgctggct gatgaaacgc cggaaggccg cagtattgtg atcctcgcca
     3541 agcaacgttt taacctgcgc gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt
     3601 ttactgcgca aagccggatg agcgggatca acatcgacaa ccgcatgatc cgtaaaggtt
     3661 ctgtcgatgc cattcgccgc catgttgagg ctaacggcgg tcacttccct gccgatgttg
     3721 atcaaaaagt cgatcaggtt gcgcgtcagg gggccacgcc gctggtggtg gtggaaggtt
     3781 cccgtgtgct gggcgttatt gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct
     3841 tcgcccagct gcgcaaaatg ggcattaaaa cggtgatgat taccggcgat aaccgtctga
     3901 ctgccgccgc cattgctgcg gaagcgggcg tcgatgattt tctcgccgaa gcgacaccgg
     3961 aggccaagct ggcattgatt cgtcagtatc aggcggaagg ccgtttggtg gcgatgaccg
     4021 gcgacggcac caacgatgct ccggcgctgg cgcaagcaga tatcgcggtg gcgatgaact
     4081 ccggcaccca ggcggcgaaa gaggcgggca atatggtcga tctcgactct aacccgacca
     4141 agttgatcga ggtggtgcat attggcaaac agatgctgat gacccgtggc tcgctgacca
     4201 ccttcagcat taccaacgat gtggcgaaat acttcgccat tattccggcg gcgttcgcgg
     4261 caacgtatcc gcagttaaat gcgctgaaca tcatgcgcct gcattcgccc gactccgcca
     4321 ttctcagtgc agtcattttc aacgccttga ttatcgtctt tttgattcct ctggcgttaa
     4381 aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt gcgccgtaac ttatggattt
     4441 acggtctggg tgggctgctg gtgccgttta ttggtatcaa agtcattgat ttactgctga
     4501 ccgtttgcgg tctggtgtga ggtttaccat gcgtggctta cgtccggcat tatcgacatt
     4561 tatctttctg ttattgatta ctggcggcgt ttacccgctg ctgaccaccg tgctggggca
     4621 atggtggttt ccctggcagg ccaacggttc gttgattcgt gaaggtgata cggtgcgggg
     4681 ttcggcatta atcgggcaga attttaccgg caacggcaga aatgccctat aatccacagg
     4741 cttctggcgg gagcaatctg gcggtcagta accctgagct ggataaacaa atagccgcac
     4801 gcgttgctgc attacgggcc gctaacccgg atgccagtac gaacgttccg gttgaactgg
     4861 tgacggcatc ggcaagcggg ctggacaata atatcacccc gcaagcggcg gcctggcaga
     4921 tcccacgcat cgcgaaagcg cgtaatctca gcgttgaaca gctcacgcag ctgatcgcaa
     4981 aatacaacca acaaccgctg gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac
     5041 tcaatctggc gctggataag cttgatgaat aacgaaccct tacgtcccga ccccgatcgt
     5101 ctgctggaac aaactgccgc gccgcatcgg gggaagctga aagttttctt cggtgcctgt
     5161 gcaggcgtcg ggaagacctg ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg
     5221 ctggatattg tggttggcgt ggtagaaacc cacgggcgaa aagataccga cgcaatgctg
     5281 gaagggctgg ctgttctgcc gccaaaacgc ctggcgtacc gtgggcggca catctgcgag
     5341 tttgacctcg atgccgccct cgcccgccgt ccggcgctga tcttaatgga cgaactggcg
     5401 cacagtaatg cgctaggttc tcgtcaccct aaacgctggc aggacatcga agagctgctg
     5461 gaagcaggca ttgatgtttt cactaccgtc aacgttcagc atctggaaag tctgaatgat
     5521 gtggtcagcg gcgtcaccgg aattcaggta cgggaaaccg tgcccgatcc ttttttcgat
     5581 gccgccgacg acgtggttct ggtggacttg cccccggacg atctgcgcca gcggctgaaa
     5641 gaaggcaaag tctatattgc cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa
     5701 ggcaatctga tcgccctgcg cgaactggca ctgcgccgta ctgccgatcg cgttgatgag
     5761 caaatgcgcg cctggcgggg gcatcctggc gaagagaaag tctggcacac gcgcgacgcg
     5821 atccttttat gcatcggcca taacaccggt agtgagaagc tggtccgcgc ggcggcgcgg
     5881 ctggcgtcac ggctgggtag cgtctggcac gcggtgtatg ttgaaacccc tgccctgcac
     5941 cgcttaccgg aaaaaaaacg tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg
     6001 ggcgcggaga cggcaacact ttctgatcca gcggaagaaa aagcagtagt gcgttatgcc
     6061 cgtgaacata atctcggcaa gattattctc ggtcgcccgg cctcgcgccg ctggtggcgt
     6121 cgggaaacgt ttgctgaccg actggcgcgc atcgcccccg atctcgatca ggtgctggtc
     6181 gcgcttgatg aaccacccgc ccgcacgatt aacaacgcgc cggatagccg ctcttttaaa
     6241 gacaagtggc gggtacagat tcagggatgc gtggttgccg ccgcgttatg cgccgttatc
     6301 actttaattg ccatgcagtg gctgatggcg tttgatgccg ccaacctggt gatgctgtat
     6361 ctgcttggcg tggtggtggt ggcgctattt tatggacgct ggccttcggt ggttgctacc
     6421 gtcattaatg tggtgagttt cgatctcttt tttatcgccc cacgcggcac gctcgccgtc
     6481 tctgatgtgc aatatctgct gaccttcgca gtgatgttaa ccgtcgggct ggtgatcggg
     6541 aaccttactg ctggcgtgcg ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca
     6601 cggcacttat atgaaatgtc gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc
     6661 gccaccatcg aacaatttat tgcctccacg tttcatgccc gcagtcaggt gttgttgccc
     6721 gatgacaacg gtaaattgca gccgttaaca catccgcaag gaatgacgcc atgggacgat
     6781 accatcgcgc agtggagttt tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta
     6841 cccggtgtac cgtaccagat tttgccgcta aaaagcggcg agaaaaccta cgggctggtg
     6901 gtggtggagc cggggaatct gcgccagttg atgatcccgg aacagcagcg cctgctggag
     6961 acgtttacgc tgttagtcgc caatgccctt gagcggctga cgctaaccgc cagcgaagaa
     7021 caggcgcgga tggcaagcga acgtgaacag atccgcaacg ccctactggc agcgctttcg
     7081 catgatttac gcacgccgct cacggtgctg ttcggtcagg cagaaatctt aacgctcgat
     7141 ctggcaagcg aaggatcgcc ccacgcccgc caggccagcg agattcgtca gcatgtgctg
     7201 aacactaccc gactggtgaa taatctactg gatatggcgc gaattcagtc cggcggcttt
     7261 aatttgaaga aagagtggtt aacgctggaa gaagtggtcg gtagcgcgct gcaaatgctg
     7321 gaaccgggtt tatcgtcgcc catcaatctt tctctgccag aaccgctgac cttaatccac
     7381 gttgacgggc cactctttga acgggtgctg attaatctgc tggagaacgc ggtgaaatat
     7441 gcgggtgcgc aggccgaaat tggtatcgat gcccacgttg agggcgaaaa tctacaactg
     7501 gatgtctggg ataacggccc cggtcttccg ccaggccagg agcagacgat atttgataag
     7561 tttgctcgcg ggaataaaga gtcggcagta ccgggggtag ggcttggact ggcaatttgt
     7621 cgggcgatag tggatgtaca cgggggcact attaccgcgt tcaaccgacc ggaaggtggt
     7681 gcctgttttc gtgttacact tccccagcaa actgcccctg aacttgaaga atttcatgag
     7741 gatatgtgac aaacgttctg attgttgaag atgaacaggc tattcgtcgc tttctgcgca
     7801 cggcgctgga gggcgacggg atgcgcgtct atgaggccga aacgctgcaa cgcggcttgc
     7861 tggaagcggc tacccgtaag ccagatttga ttattctcga tctcggcctg cccgatggtg
     7921 atgggattga gtttatccgc gacctgcgcc agtggagcgc ggtgccggtg attgtgcttt
     7981 ccgcacgcag cgaagagagc gacaaaatcg ccgcgctgga tgccggagcg gatgattatc
     8041 tgagtaagcc gtttggcatt ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc
     8101 actccgctac cgccgcgccc gatccgctgg tgaaattttc cgatgttacc gtcgatttag
     8161 ccgcccgcgt gattcaccgg ggtgaggaag aggtgcatct cacaccaatt gagttccacc
     8221 tgctggcggt gctgctcaac aatgccggaa aagtactcac ccagcgccag ctccttaacc
     8281 aggtgtgggg gccaaacgcg gtcgaacaca gtcactattt gcgtatttat atgggacatc
     8341 tgcgacaaaa actggaacag gatcccgccc gcccacgcca tttcattact gaaaccggta
     8401 ttggctatcg gtttatgctt tgaatattaa ttttaataca gcctgccttt tattaattaa
     8461 agccgtaata ataaatacgg ctttttatct taaacaacac acaaaaataa caattcaata
     8521 ttttatatta ctgagtaaaa agctcatcat taaataaatt aagattgatc attttttatt
     8581 gatcaccttc acagttcaac cgtatttcct ggatagaata tcttcacctt cattcacatc
     8641 aggaaaggta aattaaatgg aaaataacag ccgcactatg ccccatataa ggcggacaac
     8701 tcatattatg aagtttgctc atcgcaatag cttcgacttt cacttcttca atgcccgtta
     8761 gtctaccgac taagggcact tcagcgtaca ggtcttcctg actctctgta ttacaggcat
     8821 atgcctgtat tccgcagtgc tcattctctg ttaacccttg tattatcagc caccggactg
     8881 gtaagggttt ctgcattctg aaaaaagcaa tctacctgat ttatttatca gcggggtaac
     8941 tttgcttttt tccggcacga tcgatttctc attcgagaaa ttgaggacct gctattacct
     9001 aaaataaaga gatgaaaaat gtcaaaatta aaaattgcgg ttagtgattc ttgcccggac
     9061 tgttttacca cgcagcgaga atgtatctac attaatgaaa gtcgtaatat cgatgtggcg
     9121 gcaatcgttt tatcgctcaa cgatgttaca tgcggaaaac tcgatgaaat cgatgccacg
     9181 ggttatggca tcccggtatt tattgctact gaaaatcaag aacgtgtacc cgcagagtat
     9241 ttgccctgta tttcgggtgt ctttgagaat tgcgaatcgc gacgagaatt ttatggtcgt
     9301 cagttagaaa ccgctgccag ccattatgaa actcaactgc gcccaccttt cttccgcgca
     9361 ctggtcgatt atgtcaatca aggtaacagc gcgtttgatt gccctggtca tcagggcggc
     9421 gaatttttcc gtcgccatcc ggcggggaat cagtttgtgg aatactttgg tgaggcgctg
     9481 ttccgttccg acttgtgcaa cgcagacgta gcaatgggtg atctgctgat tcacgaaggc
     9541 gcgccatgca ttgcacagca acatgcggca aaagtgttca atgccgataa aacatacttc
     9601 gttctaaatg gcacttcatc ttctaacaaa gtggttttaa acgccctgct gacaccgggt
     9661 gatctggtgc tgtttgaccg caataaccac aaatctaacc accacggagc gttgctacag
     9721 gctggtgcaa caccggttta tctggaaacc gcacgtaacc cgtatggctt tatcggtggc
     9781 attgatgcgc actgttttga agaaagttac ctgcgtgagc tgatcgcgga agtcgcaccg
     9841 cagcggacaa aagaggctcg tcctttccgc ctcgccgtta ttcagctagg cacctacgac
     9901 ggtacgattt ataacgcccg ccaggtggtg gataaaattg gtcatctgtg tgactacatt
     9961 ctgtttgact ccgcatgggt cggctatgag caatttatcc cgatgatggc tgactgttca
    10021 ccgctgttgc tggatcttaa tgagaacgat ccgggcattc tggttacgca atctgtgcat
    10081 aaacagcagg ctggtttttc tcaaacttca caaatccata aaaaagacag ccacatcaaa
    10141 gggcaacagc gttatgtacc gcacaaacgc atgaacaacg cctttatgat gcatgcctcc
    10201 accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat gcatgaaggt
    10261 gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg gcattaatgc ccgcaagctg
    10321 atcctcgata actgtcagca tattcgtccg ttcgtacctg aactagtgga tggtaaaccc
    10381 tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc gcttcttcca gtttgtacca
    10441 ggggaacact ggcactcttt tgaaggctat gcagagaatc aatactttgt cgatccatgc
    10501 aaactgttgc tgacaacccc aggtattgat gcacgtaacg gcgaatatga agcgttcggt
    10561 gtacccgcga cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc
    10621 gatcttaact ccatcctctt cctgctgact ccggcagaag atatggccaa acttcagcaa
    10681 cttgttgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa
    10741 gtgctgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac ccttcgtcag
    10801 ttatgccagg aaatgcatga tttgtatgcc cgtcacaacg tgaaacaacc gcaaaaagag
    10861 atgttccgta aggaacactt cccacgcgta agcatgaatc cacaagaagc caactacgcc
    10921 tattcacgcg gtgaagtgga gctggttcgt ctgccggatg ccgaaggccg tatcgctgcc
    10981 gaaggtgcgc tt
//
LOCUS       DAJTOR010000136        10898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTOR010000136 DAJTOR010000000
VERSION     DAJTOR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.136"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(228..2087)
                     /gene="gss"
                     /locus_tag="OO249_003129"
     CDS             complement(228..2087)
                     /gene="gss"
                     /locus_tag="OO249_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5637335.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2292..3158
                     /gene="yghU"
                     /locus_tag="OO249_003130"
     CDS             2292..3158
                     /gene="yghU"
                     /locus_tag="OO249_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5637336.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPICVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3281..3529)
                     /gene="hybG"
                     /locus_tag="OO249_003131"
     CDS             complement(3281..3529)
                     /gene="hybG"
                     /locus_tag="OO249_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5637337.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3542..3883)
                     /gene="hypA"
                     /locus_tag="OO249_003132"
     CDS             complement(3542..3883)
                     /gene="hypA"
                     /locus_tag="OO249_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5637338.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3876..4364)
                     /gene="hybE"
                     /locus_tag="OO249_003133"
     CDS             complement(3876..4364)
                     /gene="hybE"
                     /locus_tag="OO249_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5637339.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4357..4851)
                     /locus_tag="OO249_003134"
     CDS             complement(4357..4851)
                     /locus_tag="OO249_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5637340.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4851..6554)
                     /gene="hybC"
                     /locus_tag="OO249_003135"
     CDS             complement(4851..6554)
                     /gene="hybC"
                     /locus_tag="OO249_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5637341.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6551..7729)
                     /gene="hybB"
                     /locus_tag="OO249_003136"
     CDS             complement(6551..7729)
                     /gene="hybB"
                     /locus_tag="OO249_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5637342.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTREELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7719..8705)
                     /gene="hybA"
                     /locus_tag="OO249_003137"
     CDS             complement(7719..8705)
                     /gene="hybA"
                     /locus_tag="OO249_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5637343.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8708..9826)
                     /gene="hybO"
                     /locus_tag="OO249_003138"
     CDS             complement(8708..9826)
                     /gene="hybO"
                     /locus_tag="OO249_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5637344.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10015..10302)
                     /locus_tag="OO249_003139"
     CDS             complement(10015..10302)
                     /locus_tag="OO249_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5637345.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10421..10898)
                     /locus_tag="OO249_003140"
                     /pseudo
     CDS             complement(10421..10898)
                     /locus_tag="OO249_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
BASE COUNT         2659 a         2991 c         2692 g         2556 t
ORIGIN      
        1 caaagtgaaa tatcattttt taacgataaa aagagtgtta tttcttgtgt atcagacaat
       61 aagcattcat tcaaaacctt tagaaataat caaaacacag gtattccact ggttaatata
      121 ttcacatatg aaatgaataa atattagaac atataaatat tgaatatttt gattaatccc
      181 cgtactgatt attcttcata atcagtacgg ggttgctaca acatgtatta ctttttcacc
      241 acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc accgcgcaaa
      301 cacgtcccac cgtagttgcc gcccacggtg aaggtacata cctgaatgta tttaccgtcc
      361 actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa tttaccgctg
      421 gttttgtcca gcacctcttc atgatggctg accaggtcga tattgctacc acagcgaccg
      481 gcaatcggtt tcactgcgta acctgttttc accaattcat cattaacagt gaaatcggta
      541 tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat cgctttgttg
      601 ccggggatca ccgtccacag cggctcaaag actagcactt ccgggcgcag caatacgtcg
      661 ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc aaactcacgg
      721 tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt ccacacgcag
      781 ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc cagttcatcc
      841 aatccacgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc cataaactgc
      901 gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg acgtgcacga
      961 ctgtgtttcc aggcaccagc caattcgtta atcagccctt ccgccggatt gaagccgttg
     1021 cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt atgacaggag
     1081 gcggagtcgg cgttgtactc gtaaaccttc aggccacgct catccatgca gaaatccata
     1141 cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg tggccagagg
     1201 attttcggaa tgtcgaacag cgccagcagg ttgtcatctt tcagtacctt gtcggttgcg
     1261 tgaagataca tcaggtgcag ctcgttggtg gctttaatta gctcctgctc ggcactctcg
     1321 gtaatggtgt agtaatgata aggatcctga ttgatcacct gaccgttggc ctgcacatag
     1381 gcgttttgca gcggatcttt ttcatccagc catttaccgt caaactggcc tttgttttcc
     1441 aggcgcgctc cgctgatttt cagcagctcg cctgcaattt ccggctgcgg taagctgtat
     1501 tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa agtgtctttc
     1561 agggtatagc agccgttttc gaccaccatc tccagctcac gcgtccactg ttgcccttgc
     1621 ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgcaattgg
     1681 gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc
     1741 gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg caggatgttg
     1801 tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat acccacgtca
     1861 gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac gcattgccac
     1921 ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc
     1981 tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc
     2041 aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta
     2101 acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt
     2161 aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa
     2221 aaaattcaca ttctgtacag caagtgacct gccacactgc ttcaacacta ccactcagaa
     2281 ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat
     2341 ccgctggcgg cgcgttcgcc aatatcaatc gcccggtttc tggtccgacg catgaaaaaa
     2401 cgctgcccgt tggcaaacac ccattgcaac tttattcgct gggaacgccg aacggtcaga
     2461 aagtaacgat tatgcttgag gagctgctgg cgctgggcgt tactggtgca gagtacgacg
     2521 cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc gaagtgaacc
     2581 caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc tgtgtgtttg
     2641 aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatt
     2701 tggcaaagcg tactgaaacg atgaactggc tgttctggtt acagggcgcg gcaccgttcc
     2761 tcggcggtgg ttttggtcac ttttaccatt acgcaccggt aaagattgag tacgccatca
     2821 accgctttac catggaagcc aaacgtctgc tcgacgtgct ggataagcaa ctggcgcagc
     2881 ataagtttgt tgcgggcgat gagtacacca ttgcggatat ggcgatttgg ccgtggtttg
     2941 gcaacgtggt gttaggtggt gtgtatgatg ccgctgagtt tcttgatgcg ggcagttata
     3001 agcatgtaca acgctgggcg aaagaagtag gcgaacgtcc ggcggtgaaa cgtgggcgta
     3061 ttgttaaccg caccaacgga ccgctgaatg agcagttgca tgagcgccat gacgccagtg
     3121 atttcgagac gaatacggaa gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa
     3181 gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca atgcctgatg
     3241 cgacgctgcc gcgtcttatc aggcctacga aagctaatca tcacgcgctg gtaatgtcgt
     3301 aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg atgatgctca
     3361 tggcaaatcc gacgtgtacc agcacccact ggcccagtag atcggcaggg ttaccttcac
     3421 aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc gcaagctggt
     3481 gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt attaactccg
     3541 gttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct
     3601 cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat
     3661 cccagcacca ggcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgccaccg
     3721 ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagcg
     3781 cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt
     3841 ggataatttc aacggcgctc tggcaaagcg acaactcatg cattttcgcc actcctgcga
     3901 ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattcgtgac tggcagcgaa
     3961 aggatcattc gtgcgcagtc atccgtcagg cgttggccct cttcaatcga catactgtgc
     4021 gaaagcggtg acatcagcga acaggagagg tattgcgaaa caccgtccag ttcgccaacg
     4081 gtaaaggtca tggtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc
     4141 ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca
     4201 caccccgtcc actgaccttc gaacagcgta aaatcagaaa catacaccgg cattgaagga
     4261 tgcagaaaag agagatcgtg catcgaacgc cgggcaattt cttcaaacgc tgcctgtact
     4321 tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgaatcg cctcccgtgg
     4381 gatggcttcc acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg caggttcaat
     4441 cattgcttca accgtcggcg ttaagccgat gtgtggctcc agcgattccg ggatcacgcc
     4501 gaccagggtc agctttttcg gaaactcacc ggtgaagcgc agggccgaca atacgtcggc
     4561 caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg
     4621 caggatcatc atcgttcccg gcgtgttctt tttcgacaca atggcatccg caataatcag
     4681 atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat
     4741 ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat
     4801 ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc
     4861 ttcactgaaa ccacttcgtt gccgtcggca tccactacgt gtaccgcaca ggccatgcac
     4921 gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc agcaaccggt
     4981 gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cggaccagag
     5041 ttccaggttg atggaacaac tgcctggtag ttactgataa taccgtcttt aataaccatc
     5101 cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt
     5161 gccggaatgt tcggtttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt
     5221 gcactgtatt gattttgcag gatatcctgc aattcgcagc agtgaacggt acgaccaata
     5281 atacggccca gcgtggagtg cagctgcgcc acttccagcg tgttgccagt cagtttctga
     5341 taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc cgccagtttc
     5401 accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc
     5461 acccaggaat atttcccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc
     5521 cacggtgcct gcggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatgcct
     5581 ttgatcaggt attcatcgga atgagaagtg atcggacgat acgaggacag atccgcattc
     5641 tcaatgtagc cgcccgggaa caggaagctg ccgtttttac tgtcggtcgg gaattccggc
     5701 acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcggca
     5761 ataactgcgg tatcaacctt ataaacctgt tcaacaaagt cgctcagctt gtcgatgaaa
     5821 gacttgatgt acatcaggcg ctcaaggttc agcacgccca aaccgtcgag gttgatcggg
     5881 ttcgcgacac cacctaccgc caggttctga atgtgcggcg ttttaccgcc cagcagcgcc
     5941 acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc
     6001 aggttcactt ccggcggcag tttcatcgcc gggtgacccc agtagccatt ggcgaaaata
     6061 cccaactgac cgctggcaac cagatctttg atcttgttct gaactttagt gaactcttcc
     6121 ggactgttca gatgccaggt cgaaacgcct ttcagcattt cggaggcttt ggttgggtca
     6181 gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaacg
     6241 atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc
     6301 ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta
     6361 gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc
     6421 acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca
     6481 ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg atcaatagta
     6541 attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag
     6601 gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata gccacaaaac
     6661 caatagaaat caacagttct tccctggtcg ggaagtaggc gtaaccgccg cccgggttga
     6721 atgccaccag cgaataggtc agacgccagg ttgcacaacc taacagtgcg ctcagtgctg
     6781 acaggaacag catgcgggaa tcattacgca gcttcgccac acgcagaacg accagcggga
     6841 agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta
     6901 acgacagctt gtcgcgatag ataagctcgc caaagcgcag cacgataaaa atcgccagca
     6961 acacactgat ggtgttggtc agcttgacga acagactctt ttcatccgga ccgttgccac
     7021 gcagacccgc ctgcaccagc gaaccttcaa agatgacaat cgagaagccc atgatgaacg
     7081 ccgtcagcag cgagaacagc ggcaacattt catagctctg ccacaacgga tgcaccttgt
     7141 agcccgccga gatcatcagt gaccccattg aagactggtg catggtcggc aacagcgcgc
     7201 caagtgcaat gatgaagaac atcaccttgt tcagacgctt gagagaaacc ttccagccca
     7261 gacgttcaaa cagtgccgga gcaaactcca gtgccatcac gccgatatag atggtcatac
     7321 agaccgccgt ctcgaacagt accgagttca cgttgaagtg acccggaatg tagaagtacg
     7381 gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacccagt gagtaaccaa
     7441 acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt
     7501 ataccgccca cgccagcgcc cagccgccac aggcaaagcc ggtaccaatc agcaggtcaa
     7561 acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag cccagaccga
     7621 acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga
     7681 ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct
     7741 ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca cggttaagcc cgccagcaca
     7801 gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga acgcgcaccg
     7861 gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc
     7921 agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc
     7981 gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgcca
     8041 ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accgaaaatc
     8101 accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc gagacgttcc
     8161 acacctttct ggttgcacag ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg
     8221 tacttcggca cgttgtacgg acaggcgacc atgcagtaac ggcaaccggt gcacacgtct
     8281 ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca gtgcagagac cgggcacaca
     8341 gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccgttc
     8401 tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cctgaatgat gttattggta
     8461 tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg
     8521 aagttgatat cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg
     8581 tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca
     8641 gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg
     8701 ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac
     8761 gcaccgccat cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag
     8821 cgcctgcaga gacgttaccg ccctctttag cgttaacatc cggtttctgt gaacgcggag
     8881 tctgattttc gacgttggca agctgatgga tgcctttatg gaagccgata ccttcttcgt
     8941 tacagccata gcatgggtga ccaatcgcta ccggccacac gccgccaaca tcgcagaatt
     9001 gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaggcacc
     9061 agccttcacg gtggccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg
     9121 ggcgacgttc gcagtgttcg tgaatcagac ggccataggc gaaggtcgga cgatttttgt
     9181 catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt
     9241 gcgggttcgg cgggcagccc ggaatattga taacggtttt gcctggcaga acttcttgca
     9301 ggctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac gcagagcagg
     9361 aaccgatagc gataatcgct gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg
     9421 gctcaccagc aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacaa
     9481 ccaacacata ctgccctttg tacttctcaa gtgcgttatg tttgttctct tcgacctgat
     9541 gaccgaaggc ggcggaaagc acttcgtgat actccagaga gatagtctcc agcacgaggt
     9601 tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgcg
     9661 cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag
     9721 cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc atgaaatcac
     9781 gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg
     9841 ttgcgaagac ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc
     9901 catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg gcgctaaaac
     9961 gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt
    10021 ttcgaggcga taaccggcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa
    10081 gaaatccatt accatctctt tatccagtcc atagcggcgc gtcagaatac ccgcttccca
    10141 ggcgctaagc tggcgatctc cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa
    10201 accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc
    10261 ttttaatccc gccattaagc ctttaccaaa atggttattc atcgttcacc tcattcgcta
    10321 tatattatca tatatagcaa tattttaggc aaagaacgac ttttgcgcac gctatttttt
    10381 catgcttgtt taagacgtaa tcccgtaaga gataaaccca tcaggagaga tatttatcga
    10441 accatttcag cgtcctttgc caggcaagat cggcggcaga tttgtcataa cgaggcgtgg
    10501 aatcattatg gaatccgtga ttaacccccg gatagatata cgcctcatag accttattat
    10561 tggctttcaa cgccgcctca taagcgggcc agccctcgtt gattcgggtg tccagttccg
    10621 cgaagtggag tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgac
    10681 cataaaacgg caccgcgcag gccagttccg gatacgcgac agccgccgcg ttcgatacac
    10741 caccgccata gcaaaatccg gtaataccca cttatgcctg tcgcttgcgg atagcgttgc
    10801 ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac ctgctgttgc
    10861 agctcacgac ctttatcatc atttcccgga taacctcc
//
LOCUS       DAJTOR010000137        10883 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTOR010000137 DAJTOR010000000
VERSION     DAJTOR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.137"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..683)
                     /locus_tag="OO249_003141"
     CDS             complement(<1..683)
                     /locus_tag="OO249_003141"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5637346.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSL"
     gene            complement(892..1551)
                     /locus_tag="OO249_003142"
     CDS             complement(892..1551)
                     /locus_tag="OO249_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5637347.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(1878..2234)
                     /locus_tag="OO249_003143"
     CDS             complement(1878..2234)
                     /locus_tag="OO249_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5637348.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(2301..2595)
                     /gene="yebG"
                     /locus_tag="OO249_003144"
                     /pseudo
     CDS             complement(2301..2595)
                     /gene="yebG"
                     /locus_tag="OO249_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            2729..3907
                     /gene="purT"
                     /locus_tag="OO249_003145"
     CDS             2729..3907
                     /gene="purT"
                     /locus_tag="OO249_003145"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5637349.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(3963..4604)
                     /gene="kdgA"
                     /locus_tag="OO249_003146"
                     /gene_synonym="eda"
     CDS             complement(3963..4604)
                     /gene="kdgA"
                     /locus_tag="OO249_003146"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5637350.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4641..6452)
                     /gene="edd"
                     /locus_tag="OO249_003147"
     CDS             complement(4641..6452)
                     /gene="edd"
                     /locus_tag="OO249_003147"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5637351.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATSGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRRFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(6687..8162)
                     /gene="zwf"
                     /locus_tag="OO249_003148"
     CDS             complement(6687..8162)
                     /gene="zwf"
                     /locus_tag="OO249_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5637352.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8499..9368
                     /gene="hexR"
                     /locus_tag="OO249_003149"
                     /gene_synonym="yebK"
     CDS             8499..9368
                     /gene="hexR"
                     /locus_tag="OO249_003149"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5637353.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9496..>10883
                     /gene="pyk"
                     /locus_tag="OO249_003150"
     CDS             9496..>10883
                     /gene="pyk"
                     /locus_tag="OO249_003150"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5637354.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQG"
BASE COUNT         2617 a         2809 c         2739 g         2718 t
ORIGIN      
        1 aggctgacgt aataggtatc gtctttttct tcgtagatca gtttatcttg cgatgctggc
       61 gtaccgatgg cgtgacgcca gacctgataa ggcagcagcg tcaccggatg cttgcgaaca
      121 tagtagaaag tccaggagtc atttgcccag acaaagctgg gttcaacgtt atccagcagt
      181 tccgggtacc agttaccagt ttccagatta cgaaaacgaa tgccgtactg gcgtcgggaa
      241 agaaaatctt ctgccagcgc cataatggtg ttatcgggcg taatcgccat tccgcccatc
      301 gaataaaact cactatgagc tgcgcgctta ttggcatcga gcaatgtttc ccactcatcc
      361 cactcttcac tgaatgccga ttgacgctgg tagatagcat attcacagcc tggttcataa
      421 atatgccgat agcggtagcc atttttgatg tagggcgcag aaacttctcg ttgcggaatg
      481 cggtcgatga tttcctttaa gatgcgatcc tgcaaggctt gttgtgaggc catcacccga
      541 tgaccgtaac tattttcttg ttgcaggtag tccaggactt ctggctgaga acgcgtatcg
      601 tcccgcagcc agtagtaatt atcgatgcgc gtatcgccat gaagcgtcat ggcgtgggga
      661 atgcgggcgg cttttggtag catgttattg ttctttctgg ttgaaacatc ttataagggt
      721 ggcaaaactc accggggatg caagcgaaac aggggagtca ttgcttagat gatgacaggt
      781 aatggcgcgg atatcgaatg ttatgcagac agagaaaatc agcctgttca aatggctgtg
      841 cgattctgga tagcccgaaa tagtcaactt caggctatcc agagagcgga attattccgc
      901 caaagtgcgt ttttgctgtt cgagatcgcg ttcaatgccg tcacgaacat cctgggggat
      961 tttcagcgcg tcacccagtg cattcaggta actgcgttcc ataaaatggt caatatcaat
     1021 agccgcgcaa ctcaggaaat agatttccag cgcctcttct tcattgcgga ccccggtagc
     1081 caagcgttgt ggatccagcg gttgttcgat tgcctgctca atgagtacac gcccctgctc
     1141 ttccacgccg gcttcacgca attgctggtc gattgccgca cgttctttgg catcaatatg
     1201 accatcactt ttagcggcaa agaccagcgc aaggatcaaa cgttctgtac gctcatccag
     1261 cggcgtactt tgcgcgccaa actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt
     1321 gtatttattc cacagcaccg tacccgctac cgctccgccg ccaaccagta acgcgttggt
     1381 gccatatttc gtaagaagtt tacgtgctga tttatttgcg accagcagcc cagccagtcc
     1441 gcctaatgcg cctggcacta acagtttgcc caatccctga tccgcagacg aggagataga
     1501 agaactgctt tgcccaagca gggattgcag ttgatttaac cagttagcca tatttgctcc
     1561 tcaataacca tttttagtgc ctgtagcata gcggatgagg atgtcagaaa atgtatatcg
     1621 agacaaaaga tgcgcaaata agcggggcgg taggccgtta ttcaaagaaa ttcgcgccag
     1681 gtaaggtatt tactgtatat caggtgataa gggtatttta ggtgaaagta gtttgtcgtt
     1741 ttgagtggca ttgtttgatg ctgattgccc gatgcgacgc tgttgcgtct tctcaggcct
     1801 acgattgtgg tgcgttctgg ggcggacaag gcactcgcgc cgcatccgcc aacggtcaat
     1861 cgaattatca gaagccatta acgccgctga tattccgcca ttcccgcttt gcagtccacg
     1921 ctgacctggt aatgaatatc ggcactttta ccacgcacgg ttagcggtac tgaccattta
     1981 tcatctttac cctgaatgtc ctgcaaactg acccaagcca cgggatcggc ctgaccgaca
     2041 attttttgat catctgccca acgcgccacg cgattttgtt gataatcacg ttttacgctc
     2101 gcggcaattc cggcagcatc cagaccttca cactttggga aagtgaccga cttgctggtt
     2161 tcattattgg cagcgaaaac tgatgcgcag gcagaaacca acaacagccc taaaaacgcc
     2221 cctctttttt tcatgttttt ctccatagca caatgattca ggagaaagca tggtacaaat
     2281 tgtcaggagc gcaagttgct tcaggccgcg tgtgaggcat cttcctcttc ggattcagca
     2341 ccgacgacct gtgggtgggg acggtaattt gccggttttc agaatggtgc tcagcacatc
     2401 tttttgttct gccagccata gcgaaagggc ttcacgttgc tcgtcttcca tttgcactgg
     2461 agaattagtc agccaggtgt caagcagatc cgccgtatca agcattttgt cataagcatc
     2521 ggcttccttt ttgctggtaa acgacatttt ctcttcgccc tcacgaatga ctacgtattt
     2581 aacttcaacc gccatttgca gcctctcata ataactgtga ttttatacag tgtatttctt
     2641 ttcggttgag aaatcaaaat caacaataaa gacacacgca aacgttttcg tttatactgc
     2701 gcgcggaatt aatcagggga tattcgttat gacgttatta ggcactgcgc tgcgtccggc
     2761 agcaactcgc gtgatgttat taggctccgg tgaactgggt aaagaagtgg caatcgagtg
     2821 tcagcgtctc ggcgtagagg tgattgccgt cgatcgctat gccgacgcac cagccatgca
     2881 tgtcgcgcat cgctcccatg tcattaatat gcttgatggt gatgcattac gccgtgtggt
     2941 tgaactggaa aaaccacatt atatcgtgcc ggagatcgaa gctattgcca ccgatatgct
     3001 gatccaactt gaagaggaag gactgaatgt tgtcccctgc gctcgcgcaa cgaaattaac
     3061 gatgaatcgc gagggtatcc gtcgcctggc ggcagaagag ctgcagctgc ccacttccac
     3121 ttatcgtttt gccgatagcg aaagcctttt ccgcgaggcg gttgctgaca ttggctatcc
     3181 ctgcattgta aaaccggtga tgagctcttc cggcaagggg cagacgttta ttcgttctgc
     3241 agagcaactt gctcaggcat ggaagtacgc tcagcaaggc ggtcgcgccg gagcgggccg
     3301 cgtaattgtt gaaggcgtcg ttaagtttga cttcgaaatt accttgctaa ccgtcagcgc
     3361 ggtggatggc gtccatttct gtgcaccggt aggtcatcgc caggaagatg gcgactaccg
     3421 tgaatcctgg caaccacagc aaatgagccc gcttgccctt gaacgtgcgc aggagattgc
     3481 ccgtaaagtg gtgctggcac tgggcggtta tggtctgttt ggtgtcgagc tgtttgtctg
     3541 tagtgatgag gtgattttca gtgaggtctc ccctcgtcca catgataccg ggatggtgac
     3601 gttaatttct caagatcttt cagagtttgc cctgcatgta cgtgccttcc tcggacttcc
     3661 ggttggcggg atccgtcagt atggtcctgc agcttctgcc gttattctgc cacaactgac
     3721 cagtcagaat gtcacgtttg ataatgtgca gaatgccgta ggcgcagatt tgcagattcg
     3781 tttatttggt aagccggaaa ttgatggcag ccgtcgtctg ggtgttgcgc tggcgaccgc
     3841 tgagagtgtt gttgacgcca ttgaacgcgc gaagcacgcc gccggacagg taaaagtaca
     3901 gggttaaacc cggcaaaaaa acgctacaaa aatgcccgat cgtagatcgg gcgttttgac
     3961 ttttacagct tagcgccttc tacagcttca cgcgccagct tagtaatgcg gtcgtaatcg
     4021 cccgcttcca gcgcatctgc cggaaccagc caggaaccac cgatgcacag cacgcttttc
     4081 agcgccaggt agtcacggta gttagccgga gaaataccac ccgtcgggca gaaacggacc
     4141 tgggagaacg gacccgcgat cgcctgcagg gctttcacgc cgccgttagc ttcagccggg
     4201 aagaatttga actctttcaa accgtagtcc atacccagca tcagttcgga aacagtgctg
     4261 atccccggaa ttagaggaat agtcccttcg gtagcagctt tcagcagcgg ctcggtcaga
     4321 cccgggctaa ttgcgaactg tgcacccgct tcagtgactt ctgccagctg ctgtggattc
     4381 agcaccgtac cggcacccac aatcgcttca ggcacttctt tggcgatagc acggatagcg
     4441 tcaactgcac actcggtacg cagagtcact tccagaacgc gcaccccacc agcaaccaac
     4501 gcttttgcca tcggcactgc gtgttccagt ttttttacca cgataaccgg tacaaccggg
     4561 ccggtggtca ggattgattc tgcacttgtt ttccagtttt tcatcagagt tttctctcgc
     4621 ctgattacaa atttgtcgtc ttaaaaagtg atacaggttg cgccctgttc ggcaccggac
     4681 agtttttcac gcaaggcgct gaataattca cgtcctgtcc ccacgcgtga cgcgctcagg
     4741 tcaggaatgt gcggttcgcg agcagccagt tccgcttcgt ctaccagcag cgtcagttcg
     4801 cctgtctgtc cattcacacg aatgatgtcc ccgtcgcgca cttttgccag cagcccgcca
     4861 tcgtaggctt ctggtgttac gtggatagct gacggcactt tacctgaagc gccggagagt
     4921 cgtccatcgg taactaacgc aattttgaaa cgccggtcca ataatacacc aagtggcggc
     4981 atgagtttat gtaattctgg cattccgttc gcttttggcc cctgatgacg gacaacaacg
     5041 acacaatcgc ggtccagcaa acccgcttca aaggccggca taacgtcatg ctggctttca
     5101 aaaacaaccg ctggcgcttc aatcacctgg ttctcaaccg gcacggcaga ggttttcata
     5161 accgcacggc ccaggttacc gcttaacact tttgtcccac catgatgaga gaaaggttgt
     5221 tcgaaggaag cgatcacatt gctgtcgagt gatttttccg ccccttcccg ccagtccagt
     5281 tcaccattat tcagccatgg ttcaagggta taacgagaca gaccaaagcc tgccaccgta
     5341 ttgacatctt catgcagcag gcctgctttg agcagttcac gcaccagaac cggtacgcca
     5401 cctgccgcct ggaagtggtt aatatcggcc ggaccgttcg ggtagagacg tgccatcagc
     5461 ggtacaacat cagaaaggtc agagaagtca tcccagttaa tctgaatacc ggccgcgcgc
     5521 gccatcgcca ccaggtgcat ggtgtggtta gtggaaccac tggtcgccag cagtgcaacg
     5581 ataccgttca ccaccacttt ctcatcgatc atcttaccga tcggcatcca ttcattacca
     5641 ttaccggtca tgcgtgtaac ctgacgcgca gcggcagcgg tcaaagcatc gcgcagcgga
     5701 gaatccggat gaacaaaaga agagcctggc gactgcatcc ccataaattc caccaccatc
     5761 tggttggtgt tggcagtacc gtagaaagta catgttcccg gcgcatggta agacgcggct
     5821 tctgactcca gtaaggccat gcggtccact ttaccttcgg cataaagctg gcgaatacgc
     5881 actttttctt tatttggcaa accgcttgcc atcggtccag acggcacaaa caccgcaggc
     5941 aaatgaccaa acgacagggc tgccatcgtc agacccggga caatcttgtc gcacacaccg
     6001 aggaacagag caccatcaaa catgttatgg gacagcccca ccgccgcaga catcgctatc
     6061 acttcgcggc ttagcagcga caattccatt ccatcctgcc cctgggtgac accatcacac
     6121 atcgccggaa caccgcccgc aacctgacca accgcattcg cttcatgcag ggctttacga
     6181 atgatttctg gatagtgttc ataaggctgg tgcgcggaga gcatgtcgtt ataggaggtg
     6241 atgatggcga tattgttacg caacatgctt ttcaaagagg ctttgtcttc tggctggcag
     6301 gcagcgaaac cgtgtgccag gttaccgcat gccaactgcg aacgatgaac ggtcgacgtt
     6361 ttcgcttgtt ctatccgggc gagataagca gagcgagtct cgcgcgaacg ttcaatgatt
     6421 cgatttgtta cgcgtaacaa ttgtggattc ataaaggctc ctgaaattga gttgtcagag
     6481 caggatgatt cacaacgcgt ttcattcaga ggatttatga gtgaaacgcc tgtaaccgga
     6541 gctcataggg caaaaacgtt tcagtcagtg taataaaaaa agcctcgtgg gtgaatccgc
     6601 acgaggcctg aaagtgtaaa aattgttcta caatctgtgc aagatcatgt taccggtaaa
     6661 ataaccataa aggataagcg cagatattac tcaaactcat tccaggaacg accatcacgg
     6721 gtaatcatcg ccaccgaggc aacgggtccc caggttccgg cctgatacgg tttcggcgca
     6781 tcattgtcca tcgcccacgc ctcagtaatg gagtctaccc atttccaggc ttcttccact
     6841 tcatcacgac gtacaaacag tgcctgaata ccacgcatgg tttccagcag cagacgttca
     6901 taggcatccg ccagatgcgt ctgattaaag gtttctgaat agctcagatc cagcttggtg
     6961 atttgcaggt tatgtttgtg gtcaaggcca ggaactttat tcagtacctg gatatccacg
     7021 ccttcatcag gttgcagacg gatagtcagt ttattctgcg gcagatcctg ccacgactct
     7081 ttaaacagat tcagttcagg tgttttgaaa tagaccacga cttcagaaca tttggtcggc
     7141 agacgtttac cagtacgcag gtagaatggc acaccggccc agcgccagtt atcaatgtcg
     7201 acgcggatcg ccacgaaggt ttctgtattg ctgctcttgt tcgcaccctc ttcttccaga
     7261 tatcccggca cttttttgcc ctgggcgaag cccgcagtat attgcccacg tacggttttt
     7321 tcgcgtacgt tggagcggtc gatgcggcgc agggacttca gtactttcac tttttcatcg
     7381 cggatgctgt ctgcgctcag gtcagatggc ggagacatcg caatcatgca gagaatttgt
     7441 agcaggtggt tctgaatcat gtcacgcatc tgaccggctt tatcaaaata gccccagcgc
     7501 ccttcgatcc ccacttcttc tgccacggta atctcaacat gatcaatggt gcgattgtcc
     7561 cagttattca caaacaggga gttagcaaaa cgcagcgcca acaggttcag caccgtttct
     7621 ttaccaagat agtggtcgat acggtaaacc tggcactcct cgaagtattc gccaacctga
     7681 tcattgattt cctgcgaggt cgccagcgac gtccccagcg gtttctccat gactacgcgt
     7741 gccggtttag cattcagttt tgcctcgcca agccctttgc aaattgcgcc aaaagtgctg
     7801 ggcggcatgg caaagtagtt aatggtgata cgattttttt gatccagcat cgcgccgaga
     7861 cggttgaatg cagcagtgtc attgacatcg agattacaaa aatccagacg tgcactcagg
     7921 gtgtcccata aaccttcatc aatggtttct ttcatgaaag tttcgagcgc ctcgcggaca
     7981 actttggtat atgccgcttt atcccagtca gcacgcccta cgccgataat ccgggtgtcc
     8041 gggttgagct gaccggcttt ttccagttga tacagggaag gcagcaattt acgacgcgca
     8101 aggtcgcctt tcgcgccgaa aatgaccagg tcacaggcct gggctgtttg cgttaccgcc
     8161 atgtcattct ccttaagtta actaacccgg tacttaagcc agggtatact tgtaattttc
     8221 ttacggtgca ctgtactgct tttacgagct tgcgaaaact gtaaacgctt atccacccgt
     8281 gcgattacgg gaaaagcgcg caaagcgcgg caaaactgat aaaaaatcat cgttttccat
     8341 tcggtaaaaa tctgacactg atcatgttat gaaaaaaaat aacaactttt ttatctgctt
     8401 ttgtcattaa cacggcacac aggcgtaata tctgacaaaa ctgcatttcg atttctttca
     8461 gtgcggaaat cgtcattacc cgtgagtctc tttacatcat gaatatgctg gaaaaaatcc
     8521 agtctcagct ggaacatttg agcaaatcag agcgcaaagt tgccgaggtc attctggctt
     8581 cgcccgataa cgcgatccat tcgagtattg ctgctatggc actggaagcc aatgttagcg
     8641 aaccgacggt gaatcgtttc tgtcgcagca tggacacgcg cggttttcct gattttaaac
     8701 ttcatctggc acagagtctg gcgaatggca ctccctatgt taatcgcaat gtcaatgaag
     8761 atgacagcgt tgaatcatac acagggaaaa tatttgagtc cgcaatggca acgcttgatc
     8821 atgtccgtca ttcactggat aaatctgcca tcaaccgcgc cgtcgacttg ctcactcagg
     8881 caaaaaaaat cgcctttttc ggattaggct cttcagccgc cgttgcccac gatgcgatga
     8941 ataagttctt tcgttttaat gttccggtgg tgtactccga tgatatcgtg ctgcaacgca
     9001 tgagttgtat gaattgtagc gacggagacg tggtggtgct gatttctcac actggaagaa
     9061 caaaaaatct ggtcgagctg gcgcagctgg ctcgcgaaaa cgacgcaatg gtgattgccc
     9121 tcacctctgc gggtaccccg ctcgcccggg aagcaacgct ggcaattacc ctcgacgtac
     9181 cggaagatac tgacatttat atgcccatgg tttctcgact tgcacagctg accgtgatag
     9241 atgtgctggc gacaggattt actttgcgac gcggtgcaaa attcagagat aacttgaagc
     9301 gggtcaagga agcgctgaag gaatcgcgtt ttgataagca gttacttaat ttaagtgacg
     9361 atcgctaaaa acgactgtca ctgtcctaat cttatacgac atccgaatga gattaattta
     9421 tcgccatcgc ggcgttattt cattcggatt tcatgttcaa gcaacacctg gttgtttcag
     9481 tcaacggagt attacatgtc cagaaggctt cgcagaacaa aaatcgttac cacgttaggc
     9541 ccagcaacag atcgcgataa taatcttgaa aaagttatcg cggcgggtgc caacgttgta
     9601 cgtatgaact tttctcacgg ctcgcctgaa gatcacaaaa tgcgcgcgga taaagttcgt
     9661 gagattgccg caaaactggg gcgtcatgtg gctattctgg gtgacctcca ggggcccaaa
     9721 atccgtgtat ccacctttaa agaaggcaaa gttttcctca atattgggga taaattcctg
     9781 ctcgacgcca acctgggtaa aggtgaaggc gacaaagaaa aagtcggtat cgactacaaa
     9841 ggcctgcctg ctgacgtcgt gcctggtgac atcctgctgc tggacgatgg tcgcgtccag
     9901 ttaaaagtac tggaagttca gggcatgaaa gtgttcaccg aagtcaccgt cggtggtccc
     9961 ctctccaaca ataaaggtat caacaaactt ggcggcggtt tgtcggctga agcgctgacc
    10021 gaaaaagaca aagcagacat taagactgcg gcgttgattg gcgtagatta cctggctgtc
    10081 tccttcccac gctgtggcga agatctgaac tatgcccgtc gcttggcacg cgatgcagga
    10141 tgtgatgcga aaattgttgc caaggttgaa cgtgcggaag ccgtttgcag ccaggatgca
    10201 atggatgaca tcatcctcgc ctctgacgtg gtaatggttg cacgtggcga cctcggtgtg
    10261 gaaattggcg acccggaact ggtcggcatt cagaaagcgt tgatccgtcg tgcgcgtcag
    10321 ctaaaccgag cggtaatcac ggcgacccag atgatggagt caatgattac taacccgatg
    10381 ccgacgcgtg cagaagtcat ggacgtagca aacgccgttc tggatggtac tgacgctgtg
    10441 atgctgtctg cagaaactgc cgcagggcag tatccgtcag aaactgttgc agccatggcg
    10501 cgcgtttgcc tgggtgcgga aaaaatcccg agcatcaacg tttctaaaca ccgtctggac
    10561 gttcagttcg acaatgtgga agaagctatt gccatgtcag caatgtacgc agctaaccac
    10621 ctgaaaggcg ttacggcgat tattaccatg accgaatcgg gtcgtaccgc gctgatgacc
    10681 tcccgtatca gctctggtct accaattttc gccatgtcgc gccatgaacg tacgctgaac
    10741 ctgactgctc tctatcgtgg cgttacgccg gtgcactttg atagcgctaa tgacggcgta
    10801 gcagctgcca gcgaagcggt taatctgctg cgcgataaag gttacttgat gtctggtgac
    10861 ctggtgattg tcacccaggg cga
//
LOCUS       DAJTOR010000138        10845 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTOR010000138 DAJTOR010000000
VERSION     DAJTOR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.138"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            296..1171
                     /locus_tag="OO249_003151"
     CDS             296..1171
                     /locus_tag="OO249_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR5637355.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKNMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1179..1955
                     /locus_tag="OO249_003152"
     CDS             1179..1955
                     /locus_tag="OO249_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR5637356.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2124..4385
                     /locus_tag="OO249_003153"
     CDS             2124..4385
                     /locus_tag="OO249_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5637357.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
     gene            4454..5629
                     /gene="senB"
                     /locus_tag="OO249_003154"
     CDS             4454..5629
                     /gene="senB"
                     /locus_tag="OO249_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR5637358.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            6088..6438
                     /locus_tag="OO249_003155"
     CDS             6088..6438
                     /locus_tag="OO249_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5637359.1"
                     /translation="MPSGRHTARIRTGHDSGTVSPFVHSTLSPLVPVYPVFITVIADH
                     DKPQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEELSNLVLLHPNCHR
                     QLHSCEAGSLKKGL"
     gene            6581..6802
                     /locus_tag="OO249_003156"
                     /pseudo
     CDS             6581..6802
                     /locus_tag="OO249_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6823..6885)
                     /locus_tag="OO249_003157"
                     /pseudo
     CDS             complement(6823..6885)
                     /locus_tag="OO249_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            7336..8748
                     /gene="ospC1"
                     /locus_tag="OO249_003158"
     CDS             7336..8748
                     /gene="ospC1"
                     /locus_tag="OO249_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5637360.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQTLFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(8852..9000)
                     /locus_tag="OO249_003159"
                     /pseudo
     CDS             complement(8852..9000)
                     /locus_tag="OO249_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            9076..10773
                     /gene="ospD3"
                     /locus_tag="OO249_003160"
     CDS             9076..10773
                     /gene="ospD3"
                     /locus_tag="OO249_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5637361.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGKLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKI"
BASE COUNT         3370 a         2286 c         2291 g         2898 t
ORIGIN      
        1 ttttcttcga catgtacatc aggcgcggaa ctcatcgggg cgcgggaatg aaatcccatt
       61 cagactccgg atagattcaa gcaagcccac ttatcgtcaa aatcggtgtt gcaaaaacgg
      121 gagtgaccat agatccgttt tccaagcgga ccccataata tcggctggta aacatcactc
      181 gctagtgcat tctttttgtt ggtaatgata attatttaca tttattcttt gatgttttat
      241 cataattaat gttttatcaa taaatatatg gaaatatatt gtttcagatg ggaatatgag
      301 aaagttcatc cttatttcta tgataacgtt agctagtgtt agtctgggag cctgcagaca
      361 gaatgtcacc ataaagcagg atgcgccagg gcagaaagct ttcctgattg acgggcagat
      421 ttacgatttg cacagtggca aaattatttc tagcagcgag cttttggcgg atcttgctac
      481 cgctcgacat cttattattg gtgaaaaaca cgataatgcc gagcatcatc agattgagct
      541 ttggcttatt caaaatctgc tcatccagcg accacagggg agtgtgttgc ttgagatgtt
      601 gacgtcagac caacagcccc gagttaacca agttaaatgt tggctgaaag ataatcctgt
      661 ggttcgagat agccgtgttc aggaactact taactggcaa aaagggtggt cctgggagat
      721 gtatggcgac atcgtcatgc agctattacg tgggccttac ccgctgctca atgccaacat
      781 tggccgggag cagatgttgg cactgtacaa aaaaaacgaa tttcctaaag gaaaaaaatc
      841 cactgcacct gtggtacaag aagctctgcg tgaaaccatc atagctatgc atgaaggcaa
      901 ccttgaaagc cagcaactga cgtcaatgct ctctgttcag cagcagcggg atcgctacat
      961 ggcccgccag cttctgagtg ccccggtacc gtcgttgttg attgcaggtg gttatcacgc
     1021 gtcaaaaaac atgggcgtac cgttacacat ggaagatctt gccaccggca cccatccagt
     1081 ggtgttgatg cttgcagaaa aaggaatgaa catcacagta gatcatgctg actacgtgtg
     1141 gtttgtagcg ccagatacaa caaaaaggta aaccatggat gatgaatatt ctccacttcc
     1201 cacagtcggt aaaatggagc agctggttta tctgttctct actgcttcat ggactgattt
     1261 tccttacgtt tatctggcgt ttttccgaag ttcagcccgc tatgtctcct gcaccggcga
     1321 tcatgctgca atgggcagaa attatagagg ctccttcttc tccactttcg cttccggtgg
     1381 gaatcgctca acaggagtcc gcagtgacag aagaaaagca acaaactgag gacagacaac
     1441 aacgtcctgt cactgaagac agtgatgcaa ccattgaaat aactaggaag aaaaagtcat
     1501 ccgatggaga aaagaaaaag acacgtccac ctcgtaagat aaaggctcaa accagcgaca
     1561 gcaaccccac agccgtttcc agtaatgctg cacctcaggc tctggtcgaa tcgtcccgta
     1621 ttgctgcgcc ttttaacagt gactcaacaa agcgagataa ttctgaggca tcttgggaaa
     1681 gcagagtaaa aggccacctt aaccgttata aacgttaccc tggcgatgcc agaaaacgtg
     1741 cacgaaccgg cacagccgtt gtgactttta ccgtaaacac ggaaggcact attgtcagta
     1801 gtttcttgga aatctcgtca ggaacccttt ctcttgaccg cgaagctatt gctgttttag
     1861 aacgcgctca accacttccc aaaccaccgc ctgaaatatt ggagggagga ttatttaaag
     1921 taaaaatgcc gattaccttt aagttgaagg agtaaaggga taattggccg ttcaagttca
     1981 tttaactgaa atttaatttt catttttatg ctgaacattt tatcaaactt ttccactgtc
     2041 gaaaaaatca aaaagacagt agatcataat taataaacaa atagttatac ttatctttat
     2101 taatattata tccaaggaaa tttatgaacg ttataaaact ggctatcggc tcaggcatat
     2161 tattgctcag ctgcggtgct tactcacaat ccatcagtga aaaaactaat tccgacaaaa
     2221 aaggagcggc agaattcagt ccgctcagcg tttctgtcgg gaagacgacc agtgagcagg
     2281 aagctctcga gaaaacaggc gcgaccagtt cccggacaac ggacaaaaac ctgcaatcac
     2341 ttgacgcaac agtgcgtagt atgcctggta cttatactca aatagatcct ggtcagggag
     2401 caatcagtgt gaatattcga ggcatgagcg gatttggtcg tgtaaacact atggtcgatg
     2461 gtattaccca gagtttttac ggaacctcta cctccggaac aacggcgcat ggttcaacta
     2521 acaatatggc tggcgtactt atagatccta acttactggt agcagttgat gttacacgcg
     2581 gtgacagcag tggctctgaa gggatcaacg cccttgccgg tagtgcaaat atgcgtacta
     2641 ttggcgttga cgatgtaata tttaacggta atacatatgg ccttcgttca cgtttctctg
     2701 tcggtagtaa tgggctggga cgcagcggaa tgatcgccct tggtggaaaa agcgacgctt
     2761 ttacggatac gggaagcatt ggcgttatgg ctgctgtgag tggcagttct gtgtactcta
     2821 atttctcaaa tggttctgga attaacagca aagagtttgg ttatgataaa tatatgaagc
     2881 agaaccccaa atcccaactg tataaaatgg atatcagacc agacgaattt aacagcttcg
     2941 aactttccgc tcgaacctat gaaaataaat ttacacgtcg tgatataacc agtgacgact
     3001 attacattaa atatcattac accccttttt ctgaattaat tgactttaac gtaacggcca
     3061 gtaccagtcg cggtaatcaa aagtatcgtg atggctcgct gtatactttc tacaaaacct
     3121 cagcgcaaaa tcgttctgac gcgctggata tcaacaatac cagccggttc actgtcgcgg
     3181 acaatgaact ggagtttatg ctgggcagca aactgatgcg tacccgctat gaccggacca
     3241 ttcactcagc ggcgggcgac ccgaaagcga atcaggaatc gatcgagaac aatccgttcg
     3301 caccctccgg ccagcaggat atttcagcgc tgtataccgg gctgaaggtt acgcgcggca
     3361 tctgggaggc agatttcaat ctcaactaca cacgtaacag gatcacaggg tacaagcccg
     3421 cctgcgattc acgcgttatc tgcgtgccac agggtagcta cgatattgac gataaagagg
     3481 gtggcttcaa cccttcagtt cagctttctg ctcaggtaac accatggctt cagccgttca
     3541 ttggctacag caaatccatg cgcgccccga acatccagga gatgttcttc tctaattcag
     3601 gaggcgcatc catgaaccca ttcctgaagc ctgaacgtgc agaaacctgg caggcgggtt
     3661 ttaacattga taccagagat ttactggtcg aacaggatgc cctgcgcttt aaggctctgg
     3721 cgtaccgcag caggatccag aactacatct acagcgagtc ttatctggtt tgttctggag
     3781 gtcgtaaatg cagtatggct gaggtgattg gcaatgactg ggagggcatt agcgatgaat
     3841 acagcgacaa tatgtacatc tacgttaact cggcaagcga cgttattgca aagggcttcg
     3901 aactggagat ggattatgat gcaggttttg cttttggccg actctctttc agccagcagc
     3961 aaacagacca gccaacctcc atcgccagca cctactttgg cgcaggggat atgaccgaac
     4021 tgcccagaaa atacatgacg ctggatactg gtgttcgctt cttcgataac gcgttgaccc
     4081 tgggcactat cataaaatac acaggcaagg ctcgtcgcct gtcgcctgat tttgagcagg
     4141 acgaacatac cggcgcaata atcaaacagg atttgccgca gatcccaacg attatcgatc
     4201 tctatggtac ttacgagtac aaccgcaacc tgacactgaa actttcggta caaaacctga
     4261 tgaacagaga ttattcggag gcgctgaata agctcaacat gatgccaggt cttggtgacg
     4321 agagccaccc agccaattcc gcgcgtggca gaacatggat atttggcggg gacattcgtt
     4381 tctgatgaca atttccgcca ggttcagaca atacgttttc tctcttatgt caattttatt
     4441 gcaggaacga aaaatgaata ttttcacttt atccaaagca ccgctatact tgttaatttc
     4501 actattttta cccacgatgg ccatggctat cgatccacct gaacgcgaac tttcgcgatt
     4561 tgccctgaaa acgaattacc ttcagtcccc tgatgaaggc gtctatgaac tggcgtttga
     4621 taatgccagt aaaaaggtgt ttgcagcagt caccgatcgt gtaaatcgtg aagccaataa
     4681 aggctatctg tattcgttta attcagattc gctgaaagtc gaaaataaat acacgatgcc
     4741 ataccgggca ttttcgctgg cgataaatca ggataaacat cagctctata tcggacacac
     4801 ccagtcagcg tccctgcgta tcagtatgtt tgacacccca accggtaaac tggtaagaac
     4861 cagcgacagg ttaagtttta aagcggcaaa cgctgcagat tcgcgttttg agcatttgcg
     4921 ccatatggtt tacagccagg attccgatac cctgtttgtg agttatagca atatgctgaa
     4981 aacggccgag ggcatgaagc ctctgcataa gctgttaatg ctcgacggga cgacgcttgc
     5041 cttaaaaggc gaggttaagg atgcttacaa aggtacagcg tatggtctga cgatggatga
     5101 aaaaacacag aaaatctacg ttggcggaag agattacatc aacgaaattg atgcgaaaaa
     5161 tcagacgctg ctgcgtacca tcccgttgaa agatccgaga ccacaaatca caagtgtgca
     5221 gaatctggcg gtggactccg cttctgaccg tgcctttgtg gtggtattcg accatgacga
     5281 tcgttccggt acaaaagatg gcctctatat ttttgactta cgcgacggta aacagcttgg
     5341 ctatgtgcac acaggagccg gagctaacgc ggtgaaatac aatccgaaat ataacgaact
     5401 gtatgtcacc aacttcacta gcggcaccat cagcgtagtg gatgccacca aatacagcat
     5461 cacccgtgaa tttaacatgc cggtctaccc aaaccagatg gtgttgtcgg acgatatgga
     5521 taccctttac attggcatca aagaaggctt taaccgcgat tgggatcctg atgtgtttgt
     5581 ggaaggagct aaagaacgta ttctgagcat tgatttgaaa aagtcgtgat cgggggaaaa
     5641 aacatgggat aaaaactccg ttattccact aaactggccg gactaggaaa agcaaacatc
     5701 tctgtcctgc cggtacgcct gttacgccgc tccatgaggc tgaggaatct ccagaaaacg
     5761 agacaggcac agcattgtac gatcaactga tttgtgccaa taattcaatg aaatcacagc
     5821 tatgcctgtt cacgtatagg aaattgaaaa actaagcgat accactgtcc gcgtcttatg
     5881 cctcgtgcgg taagctgttt ttcacatcac gccggtgttt ttttctttct gtggacggtt
     5941 ttaatactgt aatcggccgt tttttatact taccccgatt cccgggcgca cttatcctgc
     6001 atcaccccgg gatatacctc agcagactga tatcccgcgc gtttttcttc agcccctcca
     6061 cattcagtcc cgcgatggcc cggcggtata ccatccggcc gccacacagc acgcattcga
     6121 acgggtcacg actcaggaac tgtttcacca tttgtgcata gcacactttc gccactggtt
     6181 ccggtttatc ctgtttttat aacggtaatt gcagaccatg acaaaccgca gccgtcaggc
     6241 tgcctactcg agtcacaagg ctctctgtgt ccgatatgcc ggcagcgcat tacccatgaa
     6301 actggctgga acgtacacca taaggtgaag aaagttatgg ggggcggtga agaattatct
     6361 aaccttgtct tgttacatcc aaattgccat cgacagttac acagttgtga agccggctct
     6421 ctcaaaaagg gcttataaag gcttgagcgg tgtaccggga aaccggtatg caccgttctt
     6481 aggggagggc agtccagcaa tgaactgtcc ttacccgaca aacatccaaa acgaacggaa
     6541 cgtgctggaa tccgcatata acccgttgta aagtgaggaa atggacacct cacttgctca
     6601 tgagaacgcc cgcctgcggg cactgttgca gacgcaacag gacactatcc accagatggc
     6661 tgaatacaac cgcctgctct cacagcggat ggcggcttat gcttccgaaa tcaaccgact
     6721 gaaggcgctg gttgcgaaac tgcagcgtat gcagttcggt aaaagcccag aaaaacttcg
     6781 cgcaaaaacc gaacggcaga ttccattttc acgggcgatc tacgccacac aggctctggg
     6841 ttgcagtgac tgctccatta aggcgatttt gaactcataa gggaagtaac aaaaaaactg
     6901 caaaagtaca ataattatat atggccaaac taacctctat taaaaaaaca tcagtttttc
     6961 acacttagtt gagcttcatg aaaaaatcat cgaggatttc attgcagaat acacattcca
     7021 ctaactggaa gcactccata agaggaatca gagaaagtat cgttttttta tagtaaaatt
     7081 cattgctgtt caataaaatt gatataatta ttaaattatg gaaataattt cacaaaaaag
     7141 attaacttga gttcttaagc atcggatttt agatattttg aaatatctgg catcacataa
     7201 catcaaaagt atataatacc cttatctaat tacatgcagt atattgtata tatactcaaa
     7261 aattgtaatt cataccctac tatatcttgt ggggaaagat attaattaaa actgttttca
     7321 tataaggttc attttatgaa tatatcagaa acactgaact cagcaaatac ccaatgcaat
     7381 atagattcta tggataacag attacataca ttgtttccaa aagtgacatc agtgcgaaac
     7441 gctgcacaac aaactatgcc agatgaaaaa aatttaaaag atagtgcaaa tattattaaa
     7501 gatttcttta ggaaaactat agcagcacaa agttatagta gaatgttctc tcaaggctct
     7561 aactttaaat ctttaaatat agcaattgat gcaccatcag acgctaaagc ctcatttaag
     7621 gctattgagc accttgacag attatcgaag cattatatat ctgaaataag ggaaaaactt
     7681 catcctcttt ctgcagagga actcaatttg ctttcgctaa ttattaattc tgatttaatc
     7741 ttcagacatc aaagtaattc tgatttgtct gataaaattt taaacattaa gtcattcaat
     7801 aaaattcagt ctgaaggaat atgcacaaaa cgaaacacat acgctgatga tataaaaaaa
     7861 atagctaatc atgactttgt gttttttggc gttgaaatct ctagccatca gaaaaaacac
     7921 cccctgaata caaaacatca cactgttgat tttggtgcaa atgcgtatat cattgatcat
     7981 gactctccat atggatatat gacattaacc gatcactttg ataatgctat tccacctgtt
     8041 ttttaccatg agcaccaatc atttttagat aaattttcag aggttaataa agaagttagt
     8101 cgatacgtac atggaagtaa aggaattata gatgtaccaa tattcaatac taaagatatg
     8161 aagttagggc tcggattata cctgattgac tttattagaa aaagtgaaga ccaaagcttc
     8221 aaggagtttt gctatggaaa aaatcttgcc cctgtggatc tggatagaat cataaacttt
     8281 gtttttcagc cagagtacca tatacctagg atggtaagta cagaaaactt caaaaaagtt
     8341 aagattagag aaatatcctt agaggaggct gttacagcat ctaattacga agaaattaac
     8401 aagcaggtca ctaacaaaaa aattgctctc cagactcttt ttctttcgat tactaatcaa
     8461 aaagaggatg tcgccttata tatattatct aattttgaga taactagaca agatgttatt
     8521 tccataaagc atgagttgta tgatattgag tatctactta gcgctcataa ttcaagctgt
     8581 aaagtacttg agtattttat caataaggga ttggttgatg taaacacaaa gttcaaaaaa
     8641 actaatagtg gggattgtat gttggataac gcaataaaat atgagaatgc agaaatgata
     8701 aaactattat tgaaatatgg tgcaacatct gacaataaat atatttaatc aaaattgaat
     8761 atcgtttagc aaaaggcagt atttgcacaa aatatcgata tattgtttat tgtcagtatg
     8821 gctcaatgtg ataatagttg gaaagtttga tgggtttcgc cccgttgtag cggtagtcga
     8881 gctggaaggt cttcaggcac tgcttacagc gatagagcag cccccctgaa ctggaatggc
     8941 cgttccggat accccctgag tttcagagta acggggacaa accacatcaa tctttgccat
     9001 caatcatcca aagggcaaag agtacaacaa cactaagtct gcgtcacaac ccatcaatga
     9061 aaggaatata tacatatgcc atcagtaaat ttaatcccat caaggaaaat atgtttgcaa
     9121 aatatgataa ataaagacaa cgtctctgtt gagacaatcc agtctctatt gcactcaaaa
     9181 caattgccat atttttctga caagaggagt tttttattaa atctaaattg ccaagttacc
     9241 gatcactctg gaaaacttat tgtctgtcga cacttagctt cctactggat agcacagttt
     9301 aacaaaagta gtggtcacgt ggattatcat cactttgctt ttccggatga aattaaaaat
     9361 tatgtttcag tgagtgaaga agaaaaggct attaatgtgc ctgctattat ttattttgtt
     9421 gaaaacggtt catggggaga tattattttt tatattttca atgaaatgat ttttcattcc
     9481 gaaaaaagca gagcactaga aataagtaca tcaaatcaca atatggcatt aggcttgaag
     9541 attaaagaaa ctaaaaatgg gggggatttt gtcattcagc tttatgatcc caaccataca
     9601 gcaactcatt tacgagcaga gtttaacaaa tttaacttag ctaaaataaa aaaactgact
     9661 gtagataatt ttcttgatga aaaacatcag aaatgttatg gtcttatatc cgacggtatg
     9721 tctatatttg tggacagaca tactccaaca agcatgtcct ccataatcag atggcctaat
     9781 aatttacttc accccaaagt tatttatcac gcgatgcgta tgggattgac tgagctaatc
     9841 caaaaagtaa caagagtcgt acaactatct gacctttcag acaatacgtt agaattactt
     9901 ttggcagcca aaaatgacga tggtttgtca ggattgcttt tagctttaca aaatgggcat
     9961 tcagatacaa tcttagcata cggagaactc ctggaaactt ctggacttaa ccttgataaa
    10021 acggtagaac tactaactgc ggaaggaatg ggaggacgaa tatcgggttt atcccaagca
    10081 cttcaaaatg ggcatgcaga aactatcaaa acatacggaa ggcttctcaa gaagagagca
    10141 ataaatatcg aatacaataa gctgaaaaat ttgctgaccg cttattatta tgatgaagta
    10201 cacagacaga tacccggact aatgtttgct cttcaaaatg gacatgcaga tgctatacgc
    10261 gcatacggtg agctcattct tagcccccct ctcctcaact cagaggatat tgtaaatttg
    10321 ctggcctcaa ggagatatga caatgttccc ggacttctgt tagcattgaa taatggacag
    10381 gctgatgcaa tcttagctta tggtgatatc ttgaatgagg caaaacttaa cttggataaa
    10441 aaagcagagc tgttagaagc gaaagattct aatggtttat ctggattgtt tgtagccttg
    10501 cataatggat gtgtagaaac aattattgct tatgggaaaa tacttcacac tgcagacctt
    10561 actccacatc aggcatcaaa attactggca gcagaaggcc caaatggggt atctggatta
    10621 attatagctt ttcaaaatag gaattttgag gcaataaaaa cttatatggg aataataaaa
    10681 aatgaaaata ttacacctga agaaatagca gaacacttgg acaaaaaaaa tggaagtgat
    10741 tttctagaaa ttatgaagaa tataaaaatc tgaatattat ctcgtggtga tacactgaat
    10801 ttgaggtaac cggctcattt aaaccgtctg gtctgttacc tccgg
//
LOCUS       DAJTOR010000139        10802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTOR010000139 DAJTOR010000000
VERSION     DAJTOR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.139"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..252
                     /locus_tag="OO249_003161"
     CDS             <1..252
                     /locus_tag="OO249_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5637362.1"
                     /translation="MAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIR
                     RLGQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            481..1071
                     /gene="uidR"
                     /locus_tag="OO249_003162"
     CDS             481..1071
                     /gene="uidR"
                     /locus_tag="OO249_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5637363.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1459..3270
                     /gene="uidA"
                     /locus_tag="OO249_003163"
     CDS             1459..3270
                     /gene="uidA"
                     /locus_tag="OO249_003163"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5637364.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLEWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3267..4640
                     /gene="uidB"
                     /locus_tag="OO249_003164"
     CDS             3267..4640
                     /gene="uidB"
                     /locus_tag="OO249_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR5637365.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            4679..5944
                     /gene="uidC"
                     /locus_tag="OO249_003165"
     CDS             4679..5944
                     /gene="uidC"
                     /locus_tag="OO249_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5637366.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQRGLFDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(6170..7678)
                     /locus_tag="OO249_003166"
     CDS             complement(6170..7678)
                     /locus_tag="OO249_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5637367.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7779..8954)
                     /gene="manA"
                     /locus_tag="OO249_003167"
     CDS             complement(7779..8954)
                     /gene="manA"
                     /locus_tag="OO249_003167"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5637368.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9153..10799
                     /gene="fumB"
                     /locus_tag="OO249_003168"
     CDS             9153..10799
                     /gene="fumB"
                     /locus_tag="OO249_003168"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5637369.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2690 a         2533 c         2793 g         2786 t
ORIGIN      
        1 atggcatttg acctgggtga aaaaaatatt cgggtgaatg gcattgcgcc gggggcaata
       61 ttaaccgatg ccctgaaatc cgttattaca ccagaaattg aacaaaaaat gttacagcac
      121 acgccgatca gacgtctggg tcaaccgcaa gatattgcta acgcggcgct gttcctttgc
      181 tcgcctgcag ccagctgggt aagcggacaa attctcaccg tctccggtgg tggggtacag
      241 gagctcaatt aatacactaa cgaaccggta aacagccgta tgtgttgttt accgggatag
      301 cgtgtgcaat tcatcaacgt ctctgctaaa caactggcag ccaaatcacg gctattggtt
      361 aaccaatagc tggatgaaaa gcatacgaat agagtgtgcc ttcgcactat tcaacagcaa
      421 tgataggcgc tcacctgaca acgcggtaaa ctagttattc acgctaacta taatggttta
      481 atgatggata acatgcagac tgaagcacaa ccgacacgga cccggatcct caatgctgcc
      541 agagagattt tttcagaaaa tggatttcac agtgcctcga tgaaagccat ctgtaaatcc
      601 tgcgccatta gtcccgggac gctttatcac catttcatct ccaaagaagc cttgattcag
      661 gcgattatct tacaggacca ggagagggcg ctggcccgtt tccgggaacc gattgaaggg
      721 attcattttg ttgactatat ggtcgagtct attgtctctc tcacccatga agcctttggg
      781 caacgggcgc tggtggtcga aattatggcg gaagggatgc gtaacccaca ggtcgccgcc
      841 atgcttaaaa ataagcatat gacgatcact gaatttgttg cccagcggat gcgtgatgcc
      901 cagcaaaaag gcgagataag cccagacatc aacacgtcaa tgacttcacg tttactgctg
      961 gatctgacct acggtgtact ggccgatatc gaagcggaag atctggcgcg tgaagcgtcg
     1021 tttgctcagg gattacgcgc gatgattggc ggtatcttaa ccgcatcctg atcctctcat
     1081 ttcgcgggct ggtgataact gtgcccgcgt ttcatacttt aatttccctg tacaaaatac
     1141 aatctagccg ggatcggaga attccccccc aaaatattca ccgtagccat atgtcatgag
     1201 agtttatcgt tctcaagatg ctcgaacgaa cgttcgattg cttattttat ggcttctgtc
     1261 aacgctgttt taaagattaa tgcgatctat atcacgctgt gcgtattgca gattttggtt
     1321 ttttgatcgc ggtgtcagtt ctttttattt ccatttctct tccatgggtt tctcacagat
     1381 aactgtgtgc aacacagaat tggttaacta atcagattaa aggttgacca gtatttttat
     1441 ttgaatgagg agtcccttat gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa
     1501 ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga tcagcgttgg
     1561 tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt taacgatcag
     1621 ttcgccgatg cagatattcg taattatgtg ggcaacgtct ggtatcagcg cgaagtcttt
     1681 ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac
     1741 ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta tacgccattt
     1801 gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg
     1861 aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga tgaaaacggc
     1921 aagaaaaagc agtcttactt ccatgatttc tttaactatg ccgggatcca tcgcagcgta
     1981 atggtctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac gcatgttgcg
     2041 caagactgta accacgcgtc tgttgactgg caggtggtag caaatggtga tgtcagcgtt
     2101 gaactgcgtg atgcggatca gcaggtggtt gcaactggac aaggtaccag cgggactttg
     2161 caagtggtga atccccacct ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc
     2221 acagccaaaa gccagacaga gtgtgatatc tacccgctgc gcgtcggcat ccggtcagtg
     2281 gcagtgaagg acgaacagtt cctgattaac cacaaaccgt tctactttac tggctttggt
     2341 cgtcatgaag atgcggattt gcgaggcaaa ggattcgata acgtgctgat ggtgcacgat
     2401 cacgcattaa tggactggat tggggccaac tcctaccgaa cctcgcatta cccttacgct
     2461 gaagagatgc tcgagtgggc agatgaacat ggcatcgtgg tgattgatga aactgcagct
     2521 gtcggcttta acctctcttt aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg
     2581 tacagcgaag aggctgtcaa cggggaaact cagcaggcgc acttacaggc gattaaagag
     2641 ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc caacgaaccg
     2701 gatacccgtc cgcaaggtgc acgagaatat ttcgcgccac tggcggaagc aacgcgtaaa
     2761 ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat
     2821 accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa
     2881 agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctagc ctggcaggag
     2941 aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc cgggctgcac
     3001 tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac
     3061 cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt
     3121 gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aggggatctt cacccgcgac
     3181 cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat gaacttcggt
     3241 gaaaaaccgc agcagggagg caaacaatga atcaacaact ctcctggcgc accatcgtcg
     3301 gctacagcct cggtgacgtc gccaataact tcgccttcgc aatgggggcg ctcttcctgt
     3361 tgagttacta caccgacgtc gctggcgtcg gtgccgctgc ggcgggcacc atgctgttat
     3421 tggtgcgggt attcgatgcc ttcgccgacg tctttgccgg acgagtggtg gacagtgtga
     3481 atacccgctg gggaaaattc cgcccgtttt tactcttcgg tactgcgccg ttaatgatct
     3541 tcagcgtgct ggtattctgg gtaccgaccg actggagcca tagcagcaaa gtagtgtatg
     3601 catatttgac ctacatgggc ctcgggcttt gctatagcct ggtgaatatt ccttatggtt
     3661 cacttgctac cgcgatgacc caacaaccac aatcccgcgc ccgtctgggc gcggctcgtg
     3721 ggattgccgc ttcattgacc tttgtctgcc tggcatttct gatagggccg agcattaaga
     3781 actccagccc ggaagagatg gtgtcggtat accatttctg gacgattgtg ctggcgattg
     3841 ccggaatggt gctttacttc atctgcttca aatcgacgcg tgagaatgtg gtacgtatcg
     3901 ttgcgcagcc gtcattgaag atcagtctgc aaaccctgaa acggaatcgc ccgctgttta
     3961 tgttgtgcat cggtgcgctg tgtgtgctga tttcgacctt tgcggtcagc gcctcgtcgt
     4021 tgttctacga gcgctatgtg ttaaatgata ccgggctgtt cactgtgctg gtactggtgc
     4081 aaaacttggt tggtactgtg gcatcggcac cgctggtgcc ggggatggtc gcgaggagcg
     4141 gtaaaaagaa taccttcctg attggcgctt tgctgggaac ctgcggttat ctgctattct
     4201 tctgggtttc cgtctggtca ctgccggtgg cgttggttgc gttggccatc gcttcaattg
     4261 gtcagggcgt tactatgact gtgatgtggg cactggaagc tgataccgta gaatacggtg
     4321 aatacctgac cggcgtgcga attgaagggc tcacctattc actattctca tttacccgta
     4381 aatgcggtca ggcaatcgga ggttcaattc ctgcctttat tttgggatta agcggatata
     4441 tcgccaatca ggtgcaaacg ccggaagtta ttatgggcat ccgcacatca attgccttag
     4501 taccttgcgg atttatgcta ctggcattcg ttattatctg gttttatccg ctcacggata
     4561 aaaaattcaa agaaatcgtg gttgaaattg ataatcgtaa aaaagtgcag cagcaattaa
     4621 tcagcgatat cactaattaa tattcaataa aaataatcag aacatcaaag gtgcaactat
     4681 gagaaaaata gtggccatgg ccgttatttg cctgacggct gcctctggcc ttacctctgc
     4741 ttatgcggcg caactggctg acgatgaagc gggactacgc atcagactga aaaacgaatt
     4801 gcgcagggcg gataagccca gtgctggcgc gggaagagat atttacgcat gggtacagag
     4861 aggattgttc gatttcaata gtggttatta ttccaatatt attggcgttg aaggcggggc
     4921 atattatgtt tataaattag gtgctcgtgc tgatatgagt acccggtggt atcttgatgg
     4981 tgataaaagt tttggctttg ccctgggggc agtaaaaata aaaccctgtg aaaatagcct
     5041 gcttaaatta ggtcgcttcg ggacggatta tagttatggt agcttacctt atcgtattcc
     5101 gttaatggct ggcagttcgc aacgtacatt accgacagtt tctgaaggag cattaggtta
     5161 ttgggcttta acaccaaata ttgatctgtg gggaatgtgg cgttcacgag tatttttatg
     5221 gactgattca acaaccggta ttcgtgatga aggggtgtat aacagccaga cgggaaaata
     5281 cgataaacat cgcgcacgtt cttttttagc cgccagttgg catgatgata ccagtcgcta
     5341 ttctctgggg gcatcggtac agaaagatgt ttccaatcag atacaaagta ttctcgagaa
     5401 aagcataccg ctcgacccga attatacgtt gaaaggggaa ttgctcggct tttacgcgca
     5461 gctcgaaggt ttaagtcgta ataccagcca gcccaatgaa acggcgttgg ttagtggaca
     5521 attgacctgg aatgcgccgt ggggaagcgt atttggcagt ggtggttatt tgcggcatgc
     5581 aatgaatggt gccgtggtgg ataccgacat tggctatccc ttttcattaa gtcttgatcg
     5641 taaccgtgaa ggaatgcagt cctggcaatt gggcgtcaac tatcgtttaa cgccgcaatt
     5701 tacactgaca tttgcaccga ttgtgactcg cggctatgaa tccagtaaac gagatgtgcg
     5761 gattgaaggc acgggtatct taggtggtat gaactatcgg gtcagcgaag ggccgttaca
     5821 agggatgaat ttctttcttg ctgccgataa agggcgggaa aagcgcgatg gcagtacgct
     5881 gggcgatcgc ctgaattact gggatgtgaa aatgagtatt cagtatgact ttatgctgaa
     5941 gtaagaaata acgccggaga gaaaaacctc cggcgtttca gattgttgac aaagtgcgcg
     6001 ttttttatgc cggatgcggc gtaaacgcct tatccagcct acaaaaactc ataaattcaa
     6061 agtattgcag gaaaaggtag gcctgataag cgtagcgcat caggcaatct ctggtttgtt
     6121 ttcagatgaa aacgccgaag tgaaaattct ccggcgtttt ggccgtgaat tactgctgcg
     6181 gaattgccgg tacagccgga acgttaagag ctggcatcgc aaacatgcca acaaaatctt
     6241 ctaacgacat tttctgccca tttaacgtta tctgaccgtt agtatattgc aggctggtgg
     6301 tgatggtatt gtcctgcaag gtggtcagac ggaacatctg ccccattgct gatgcacctt
     6361 caacttgctg tttcgccagt tttttcgctt gatcttcctg ataaccttcc agcttcgcta
     6421 cctgagtcat aaactcagtt gccatatcca ccggaatggt cagtttcgca tccagagatt
     6481 taaccgaacg atctacttcc tgcgccagcg tttgcggcgc ttctttagtc gttgccggat
     6541 ctttcaggaa cagcgacaga ttcagcgcac tttcaccctg actgtttttc cagcttagcg
     6601 gcgcgacagt aatcaccgga tcgcctttca acatcagcgg cagggcgcta aagaaggctt
     6661 ccgtcacttt ctcctgataa agttcgggat tgttggcaat ttctggctgt gccagcagcg
     6721 cctgagtttg cgcgttatat tgctggctga actgatgcca ggcttcacca tcaatctggc
     6781 cgacttttaa agtcagcttg ccgctgccca gatcctgatt ctgtaccttc aggctgttta
     6841 gcgagtaatc cagttggcta ttgatcgttt taccgtcatt gaccagatcc gatttaccgc
     6901 tgatctccat gccttccagc agtgccagtt ctttgccttc cactgaaatg gtcatttttt
     6961 ccagtgacag tttttggttt cctacgcgct caccaaaact tgccagcgtg ctggaaccgt
     7021 cggttttcag attattaaag gtcaactgca ctttctggtt gtattcgtta actgcgtcta
     7081 tccgaccact ttgcgcctcc ccggaaaggg agatggcttt gccgtctctg tcagcattta
     7141 actggaactc gccgccgcta aaggcgactt tttcatcctt ttgctcgtaa ttcagtggct
     7201 tgagcgaaat atcggaactg gaatcaccgc tgtaaccaat gcgcgagtta atctcaaaag
     7261 gcgtttcacc ttttgccata tcaaacagtg gtttgcttac ttcgttatta accagcgtgg
     7321 tttgaattga tgccatcgac gggatcaggt tcagtttttt aagctgggca agcgggaagg
     7381 gaccatgatc aaccgattcg ttgaagatga cgctctgacc gcttttaatc cacggatttt
     7441 ctttcccggc aatgggtttc accaacagtt gcaactggct gctgaatacg ccgcgatgat
     7501 agttttgata actcacttcc aggttggatt caggagctgt cagttttagt tgcgcgttcg
     7561 cctgcgcgac catgtcttcg agatgggttt caatcttctt gcctgtatac catgcgccgc
     7621 ctgtccagac tacgcctagc gcaacaatga cgcctaccgc taccagcgat ttattcataa
     7681 tgattatcca taaaatgaaa tcaggcggac tggccgcctg aaggtgttat aagcctttaa
     7741 taagcttagc aagagatgtt aattttttca gtaagctctt acagcttgtt gtaaacacgc
     7801 gctaaacggc cgtggccttt gacagtcacc ggtgattcgt tggcggcaat aaacgctgat
     7861 tcacccggtt taagctgtaa ctgctgagaa cctttccaca acgttgcatc gccttcgacg
     7921 cagaacaaaa tggcggcact ctgctggcta atggtggttt ctttatcact aaggtcatgc
     7981 agcgagaagg caaaatcatc cactggaatc gggaagtcca gttctgcacc ttgtttcacc
     8041 ggctgggtca acaactggtt agccggtttg gcttcgaatt tcacattggc aaccagttcc
     8101 ggaatatcaa tgtacttagg cgtcagaccc gcacgcagca cgttatcgga gttcgccatc
     8161 acttccagcg ccacgccttg caggtaagcg tgcggtgttt cagcgaacag gaacatcgct
     8221 tcgccaggat tcaatttcac cacattcagt aacagcgggg agaacaggcc gctgtcttcc
     8281 gggtaaaatt cagaaattaa acgaatcgtt tgccacggtt caccctgctg actgtcgagc
     8341 gccgatttta aaatcgccag cgcgcgggat ttttcttcac cctgcatatt caacaggctg
     8401 gcgaacagtt cgcttaaacg ttcggcatta ggctgttgta aaaagtgagc aattgccgga
     8461 tgtgcacctg cgactggctg gagtagggag acaatctcgg aaaactcacg aaacgcgttc
     8521 atcgcaagga aaggcgtcag cgcaaaaacc agctccggct tgtggttagg atctttatag
     8581 ttacgctcgg cggcatccat cgggatacct gcggcatttt ctttggcaaa accgatttca
     8641 gaattgcgtt tgtttggatg aacctgaatg gagagcggct gtgctgcgca taatactttg
     8701 aacaggaaag gcagttcgcc aaagcgtttg gcaacggcct ctccgagcag agtcgattta
     8761 tcactctcaa tcacatcacg cagtgaaacg atatctccgg cggcattctg cactcgtgaa
     8821 ctgcttttcg gatgtgcgcc catccacagc tcggccatcg gctggctgga cggattttcc
     8881 ataccataaa gttcagtcaa cgccgttttg ctgccccagg catagttttg cactgagtta
     8941 atgagttttt gcatgatcaa tccctgtttt aatgtggaaa ttaatcccac tattaaagca
     9001 agaatcctac gggaagtaac ctggagccgc aaaaagtcgt actagtctca gtttttgtta
     9061 aaaaagtgtg taggatattg ttactcgctt ttaacagggc aacggaacac ccgcccagag
     9121 cataaccaaa ccaggcagta agtgagagaa caatgtcaaa caaacccttt cattatcagg
     9181 ctccttttcc actcaaaaaa gatgatactg agtattacct gctaaccagc gaacacgtta
     9241 gcgtatctga atttgaaggg caggagattt tgaaagtcgc acccgaagcg ttaactctgt
     9301 tggcgcgtca ggcgtttcat gatgcgtcat ttatgctgcg tccggctcac caacaacagg
     9361 tggccgacat tctgcgtgac ccggaagcca gcgaaaatga taaatatgtg gcgctgcaat
     9421 tcctgcgcaa ctccgacatc gcggcgaaag gtgttctgcc aacctgtcag gataccggca
     9481 ctgcgattat tgttggtaaa aaagggcagc gtgtatggac cggtggctgt gatgaagcgg
     9541 cgctggcgcg cggtgtctat aacacttata tcgaagataa tttgcgctac tcgcaaaacg
     9601 cgccgctgga tatgtataaa gaggtgaata ccggcaccaa tctgccagcg cagatcgatc
     9661 tttatgccgt tgatggcgac gagtacaaat tcctctgtat cgccaaaggt ggtggttcgg
     9721 caaacaagac gtatctctat caggaaacca aagcgttact aacgccgggg aaactgaaaa
     9781 attacctggt tgagaagatg cgcacgctgg gtacggcggc ctgtcctccg tatcatattg
     9841 cgttcgtaat tggtggaact tctgcagaaa ctaaccttaa aacagtgaaa ctggcttccg
     9901 ctaaatacta tgacgaactg ccaacggaag ggaatgagca cggtcaggca ttccgcgatg
     9961 tggaactgga aaaagaattg ctgatcgaag cgcaaaatct cggtctgggt gcgcagtttg
    10021 gtggtaaata cttcgctcac gacatacgcg tgattcgcct gccacgtcac ggcgcatcct
    10081 gtccggttgg tatgggtgtt tcctgctctg ctgaccgtaa tatcaaagcg aagatcaacc
    10141 gtcaggggat ctggatcgaa aaactggaac ataatccagg taaatatatc cctgaagagc
    10201 tgcgtaaagc gggagaaggc gaagcggtgc gcgttgacct taaccgtccg atgaaagaga
    10261 tcctcgcaca gttgtcgcag tatcccgttt ctacacgctt atcgcttaac ggcacgatta
    10321 tcgtcggtcg tgatattgct cacgccaaac tgaaagagcg gatggataac ggtgaagggc
    10381 tgccgcagta cattaaagat catccgattt actacgcggg tccggccaaa acgccggaag
    10441 gttatgcctc cggttctctt ggcccaacga ccgccggacg gatggattct tatgtcgatc
    10501 aactgcaagc gcagggcgga agtatgatca tgctggcgaa aggcaaccgc agccagcagg
    10561 tgacggatgc ctgtaaaaaa cacggcggct tctaccttgg cagtatcggt ggtccggccg
    10621 ctgtattggc gcagggaagt attaagagcc tggaatgtgt tgaatatccg gaactgggaa
    10681 tggaagccat ctggaaaatt gaagtggaag atttcccggc gtttatcctt gtggatgata
    10741 aaggaaatga cttcttccag cagatacaac tcacacagtg cactcgctgt gtgaaataaa
    10801 ca
//
LOCUS       DAJTOR010000140        10744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTOR010000140 DAJTOR010000000
VERSION     DAJTOR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.140"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(90..239)
                     /gene="hokB"
                     /locus_tag="OO249_003169"
     CDS             complement(90..239)
                     /gene="hokB"
                     /locus_tag="OO249_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5637370.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            638..2251
                     /gene="trg"
                     /locus_tag="OO249_003170"
                     /pseudo
     CDS             638..2251
                     /gene="trg"
                     /locus_tag="OO249_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(2289..3211)
                     /locus_tag="OO249_003171"
                     /pseudo
     CDS             complement(2289..3211)
                     /locus_tag="OO249_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3428..4771
                     /locus_tag="OO249_003172"
     CDS             3428..4771
                     /locus_tag="OO249_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5637371.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGPLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GGPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEYRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4996..6651
                     /gene="opgD"
                     /locus_tag="OO249_003173"
                     /gene_synonym="mdoD"
     CDS             4996..6651
                     /gene="opgD"
                     /locus_tag="OO249_003173"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5637372.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6791..7015
                     /locus_tag="OO249_003174"
     CDS             6791..7015
                     /locus_tag="OO249_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5637373.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            7078..7614
                     /gene="rimL"
                     /locus_tag="OO249_003175"
     CDS             7078..7614
                     /gene="rimL"
                     /locus_tag="OO249_003175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5637374.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7609..8589)
                     /locus_tag="OO249_003176"
                     /pseudo
     CDS             complement(7609..8589)
                     /locus_tag="OO249_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8713..9705
                     /gene="tehA"
                     /locus_tag="OO249_003177"
     CDS             8713..9705
                     /gene="tehA"
                     /locus_tag="OO249_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5637375.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9702..10295
                     /gene="tehB"
                     /locus_tag="OO249_003178"
     CDS             9702..10295
                     /gene="tehB"
                     /locus_tag="OO249_003178"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5637376.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10598..10720
                     /locus_tag="OO249_003179"
                     /pseudo
     CDS             10598..10720
                     /locus_tag="OO249_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT         2749 a         2502 c         2722 g         2771 t
ORIGIN      
        1 aaaacatgca ctaactgaag tgtaaaaggg gtgccatgag aaaatgaatc tgctagtcag
       61 atgcgcgggg aaaatccccg cgcttgccct tacctggacg tgcaggccat gagcgcagca
      121 acctccttat caccgtcccg gaaccgcagt tcgtagagcg tttgtcgggt caggagtgtg
      181 aatgtcagaa tcgtaatgca gataatgagc agacacacca ccagagggtt gtgcttcata
      241 gcctttctcc ttgccggatg gcgggtaaga ggctaagatc tgaattgcta ggttcattcg
      301 ttggcctcgg ttgatagaaa tatcggtcgg ggccttcgtc tttctgattc ccggttagcc
      361 tgaaaacaga aagtctcagg cacccgcagg catcctatga ggtttcctta gggacgaaaa
      421 taatcacttc acgaaattgc gtgctgtttt ccagaatttt tcgtcattcg gattagccag
      481 tttagccatt cgttactctc ttcattccaa tagcattaat tttctctgca ataattgttg
      541 taaaaatgtg acgcaaagag gtttttggtc ataagtaatt accgtcaagt gccgatgact
      601 ttctatcagg agtaaacctg gacgagagac aacggtaatg aatacaactc cctcacagcg
      661 attaggtttt ttgcatcaca tcaggctggt tccgttattt gcctgcattc ttggcggtat
      721 cttagttcta ttcgcattaa gttctgctct ggcaggccga tcgcgatcag cgtgatgtta
      781 ctgcggagat tgagattcgg accgggttag cgaacagttc agattttttg cgttcagccc
      841 ggatcaatat gattcaggcc ggggctgcga gtcgtattgc ggaaatggaa gcaatgaagc
      901 gaaatattgc gcaagccgaa tcggagatta aacagtcgca gcaaggttat cgtgcttatc
      961 agaatcgacc ggtgaaaaca cctgctgatg aagccctcga cactgaatta aatcaacgct
     1021 ttcaggctta tatcacgggt atgcaaccta tgttgaaata tgccaaaaat ggcatgtttg
     1081 aagcgattat caatcatgaa agtgagcaga tccgaccgct ggataatgct tataccgata
     1141 ttttgaacaa agccgttaag atacgtagca ccagagccaa ccaactggcg gaactggccc
     1201 atcagcgcac ccgcctgggt gggatgttca tgattggcgc gtttgtgcgt gccctggtca
     1261 tgacgctgat aacatttatg gtgctacgtc ggatcgtcat tcgtccactg caacatgccg
     1321 cacaacggat tgaaaaaatc gccagtggcg atctgacgat gaatgatgaa tcggcgggtc
     1381 gtaatgaaat cggtcgctta agtcgtcatt tacagcaaat gcagcattca ctggggatga
     1441 cagtagggac tgttcgacag ggcgcggaag agatttatcg tggcaccagc gaaatttcag
     1501 ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc ggcggctatc gaacaaactg
     1561 ccgccagcat ggagcaactc actgcgacgg tgaaacagaa tgcggataac gcgcatcatg
     1621 ccagcaaact ggcgcaagag gcttctatta aagccagcga tggcgggcag acggagtaaa
     1681 aacgatgggc gctatctcca cgagttcgaa gaaaatttct gagatcaccg ccgtcatcaa
     1741 cagtattgct ttccagacga atattctggc actgaatgct gccgttgaag ccgcgcgagc
     1801 gggtgagcaa gggcgtggat ttgccgttgt cgccagcgaa gtacggacac tcgcaagtcg
     1861 cagcgctcag gcggcgaaag agattgaagg cttgatcagt gaatcagtca ggttaattga
     1921 cctggggtcg gatgaggtgg caacggccgg gaaaaccatg agcactattg ttgatgccgt
     1981 cgcgagtgtc acacatatca tgcaggaaat cgccgccgcc tcggatgaac aaagtagagg
     2041 cataacgcag gttagccagg cgatttctga aatggataag gtgacgcaac agaatgcttc
     2101 tctggtagaa gaggcctcag cggcggcggt gtcccttgaa gaacaggcgg cacgattaac
     2161 tgaggcggtg gatgtattcc gtctgaacaa acattctgtg tcggcagaac ctcgcggagc
     2221 gggtgaacca gttagtttcg ctacggtgtg aaaatgttca aggagggatc gacagatccc
     2281 ttcacctttc agaacggcat tgattttcga atagcgttaa tcatcaactg gcaaccagaa
     2341 gagaacatcg catctacgcg ggtcagtatt ccaatcggtt cgcctgcacc atgtcccgga
     2401 acaggctggg ccaccagcgt ggcatgacgc aggtcgtctt ttaccgcgcc agaagggaca
     2461 aaccacacgt agtcgtattc aaccgtaagt tgacgagata gcgaagcaga cagcgtttcg
     2521 atacaacccg aaggaatttt acatccctgg ctctgtacta atgcatctga atgctggcgt
     2581 ggcgcagtgc cttctggtga tacaacgacc ggccatttca gcactcggct tagcgttacg
     2641 ttctcctgaa gtagcgggtg attagggcgg acaaccagct tcagcgattc aagaaacagc
     2701 agttcgtaat taagcccggt catcagttca ggatctgaca tccgaccaat gccgatatcg
     2761 atttccccgg ttttcaaacc cgccagaatc atagggttac tcattgtcgc aacttgcaag
     2821 gtcgtctctt tttgttgctg atgaaactga cctataaccg aaggtaatat ccccagtgcc
     2881 gcagtaggta gtgcaccaat cctgacgaca tcattattaa gaccttcttt acgatgaagc
     2941 gactgtccgg cagtgttgat ggcgtcaaga actctgactg catgcgttaa aaattgttcg
     3001 ccgggtaagg taagttgcgc cccctgacga ccacgctcaa acaagcgagc gcccgtcagc
     3061 tgctacagtt cattcaatgt cttagagagc gcaggttgac tcaaattaag ggtttcagcc
     3121 gcgcccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     3181 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     3241 tgacaagtga agttgtttga ttatgataac ttgattgcaa aatataatta acaattaaag
     3301 caattgtgtt acagcaaaat ggataatatt gatgttttcg cggcgagatc acagtttgta
     3361 aattcttccc gccagagtga atgcggttac ctacactcca gattactgac cactggaggc
     3421 agacactatg gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat
     3481 gtcagccatg taccagcaag aagttccgca gtacggcccg ctgctggaac tggtagctga
     3541 tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga
     3601 tgagctggcg cgactgaatg ttgaacgtca tggggcgatt cgcgttggga ctgcacaaga
     3661 gcttgctact cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga
     3721 tctctcgcag gcaggggggc cggtacattc gacagcattt cggcccattg atgatgcttc
     3781 tctggcgcgt aatcccttcc gcgtttttac ctccttactc cgccttgagc ttatcgagaa
     3841 cgaaattttg cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg
     3901 ttgtcgacaa ctgttagagg aatatgagca gcagggcggt tttaacgaaa cacaggcaca
     3961 ggaatttgtg caagaggccc tggaaacgtt tcgctggcac cagtcagcaa cggtagatga
     4021 agaaacctat cgcgccttgc acaacgaata tcggttgatt gctgatgtgg tctgttttcc
     4081 tggatgccat atcaaccacc tgacgccacg tacgctggat attgaccggg tgcagtcgat
     4141 gatgcctgaa tgcggaattg aacccaaaat tctcatcgag gggccgccgc gccgcgaggt
     4201 accgatttta ctacgccaga ccagctttaa agcactggaa gagacggtgt tgtttgcggg
     4261 gcagaaacag ggcacgcata ccgcgcgctt tggtgaaatt gagcagcgtg gcgtggcatt
     4321 aacgccgaaa gggcgacaac tgtatgatga tcttctgcgt aacgctggaa ccgggcagga
     4381 taatctcact caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt
     4441 tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt cgggtgaggc
     4501 gcatcgtcag gcgattcatc ttggagacga tccacagcct ttaattgaac gtggttgggt
     4561 agtggcgcaa cccatcacct atgaagattt cttgcccgtt agcgcggcgg ggatcttcca
     4621 gtcaaatctg ggtaatgaaa cgcagacacg cagtcacggt aatgccagtc gcgaagcatt
     4681 tgagcaggcg ttgggttgtc cggttttgga tgagttccag ctttaccagg aagcggaaga
     4741 acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt
     4801 gcctgatttt acaaacgaat caggctcatc ccatcgacat aaaaaaaatg ccgatttatg
     4861 catattctct cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat
     4921 aaataagcac tttcttgccg ctgaaaacga ccagcgcggg accattcaca acaccagaag
     4981 gactcacttt caggtatgga tcgtagacga tttattaaag gttcaatggc tatggccgcc
     5041 gtgtgcggta ccagcggcat tgcttctctt ttttctcagg cggcattcgc ggcagattct
     5101 gatattgccg acgggcaaac ccagcgtttt gacttctcca ttctacagtc aatggcgcac
     5161 gacttagcgc aaacagcgtg gcgtggtgcg cctcgtccgt tacctgacac gctggcgaca
     5221 atgacgccgc aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac
     5281 gttgagaacc gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc
     5341 gttcgtatgt tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg
     5401 gagttgttca aatacaacga tgcgggtgtt gatacaaaac aattagaagg gcaaagcgat
     5461 ctcggctttg ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtagta
     5521 tcatttctcg gcgcgagtta tttccgcgcc gttgatgata catatcaata cggtttgtcg
     5581 gcccgcggcc tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttacc
     5641 gccttctggt ttgatacggt aaaaccgggg gcaactacct ttaccgttta tgcgttgctc
     5701 gatagcgcca gcattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg
     5761 attatggatg tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcattgcg
     5821 ccgatgacca gtatgttcag ctgcggtact aatgaacgtc ggatgtgcga tacaattcat
     5881 ccgcaaattc atgactctga tcgtctgtcc atgtggcggg gcaacggcga gtggatttgc
     5941 cgtccgctga ataatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa
     6001 gggtttggtt tattgcaact ggatcgtgac ttctcccatt atcaggacat tatgggctgg
     6061 tataacaaac gcccaagtct gtgggtggaa ccgcgtaaca agtggggtaa gggcaccatc
     6121 ggcctgatgg aaatcccaac aacgggcgaa acgctggata acattgtctg tttctggcag
     6181 ccagaaaaag ctgtaaaagc aggtgatgag tttgcattcc agtatcgtct gtactggagt
     6241 gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc
     6301 ggtttcccgg aaggttgggc gccaggtgaa cactatcccg aaaaatgggc gcgtcgtttt
     6361 gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg
     6421 attacgcttt ccagtgggga agcgaagcaa atcgaaattc tctatattga acccatcgat
     6481 ggttatcgta ttcagtttga ctggtatccg acttcggact ccactgatcc ggtcgatatg
     6541 cggatgtatc tacgttgtca gggggacgct atcagtgaaa catggctgta tcagtatttc
     6601 ccgccagcgc cggataaacg tcagtatgtt gacgaccgcg tgatgagtta attgtttttt
     6661 cttcggcacc ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc
     6721 ctaagttaaa tgagtaatct gatggtgtgt atttcagata caccttgtca gccactaaca
     6781 gggagtgcgt atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc
     6841 tcgctttatg tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga
     6901 aggtttcgac ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca
     6961 gttaaaagat gagatgctca aaatcctgca gcaggagagc gtcaaagggg tgtaaacttt
     7021 cctaagccgc cagccaggcg gcttttttaa caacagcatg gattgactgg agataagatg
     7081 actgaaacga taaaagtaag cgaatcactt gaattacatg ctgttgcgga aagtcacgtc
     7141 acacctcttt atcagttaat ctgtaaaaat aaaacctggt tacagcagtc gctaaactgg
     7201 ccgcagttcg ttcagagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg
     7261 catcaacgcg gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt
     7321 atctcgttta atcgtattga accactgaat aaaactgctg aaatcggcta ctggctggac
     7381 gaatctcatc aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac
     7441 gctcagtctg gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tccgcaaagc
     7501 aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt gcctgaaaca ggctgagttc
     7561 ctgaatgatg cctatgatga tgtgaactta tacgcgcgta ttatcgattc ataataaccc
     7621 tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg
     7681 aaccgtgtcg ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catggttttc
     7741 aattatcacc gcacctgaaa tacggctctc accttgtatt acaacgtgct cgtcgagcag
     7801 aatcggttca ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca
     7861 attaccttcc acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc
     7921 atcttcttct atgccagctt tcacctgcgc gtgaccgtag actttagcgc aatcgcatag
     7981 ccagacgttg ttctcttcat taccttcaac actggcaaaa tcaaaaactt cggcgcgatg
     8041 ttcaataaaa gcataccgga cgacggcatc gccataaatt tgtgcctggt ggacaatacg
     8101 cgaggcgcta acccttgcgc gatcataaat ttggagcagg agttgatggt cgggcgttaa
     8161 gccttgtgcg gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca
     8221 ttgcccgtaa actaatgagt catgaatggt gacactgtca cttatatatg caccctgact
     8281 gatttcacta ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagcacgct
     8341 ggtgccggtg atacgcgtat taccagaaat caccgttccg ccgaacgcaa tagcattctg
     8401 gtcgtaaatc cagcaatttc cttcctatgc taacaccgtt tcgcgatcga tccagccgcc
     8461 agcggttcct gctatcacat cgttaaaatc gctaatggcg ataatctgcc gtaataacac
     8521 acttttttta gtgccatctt cctgataact aaaggctcgc tgttcttcac ttagacgata
     8581 tttgcgcata gcgttttctc acaggtgact tactataacc gtagcaaatt ctgcggctct
     8641 ggctatgctc gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa
     8701 atgagtaaga agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt
     8761 gtgttgggga cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc
     8821 agccactggt taggggatgg gctggtgatt ctggcgatga tcatctgggg attattaact
     8881 agcgcattta ttacccgact catacgcttt ccgcatagcg tgctggcgga agttcgccat
     8941 ccagtgctga gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt
     9001 tttgttccgt ggtttcgccc actggcggtg tgcctgttca gttttggtgt cgtggttcag
     9061 ttggcttatg ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct
     9121 accatgcctg gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt
     9181 ggtgcgttgg gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg
     9241 ctaagccttg aaccggtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggca
     9301 ctgcggacat cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggttg
     9361 agcgtcaacg gcggcgaggg tgatacgctg gcgaaaatgc tttttggtta tggactgctg
     9421 caactgctgt ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca
     9481 ttctggagtt tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt
     9541 ggcagcgaaa atggattttt ccatacgctg gcggtaccgc tgtttatctt taccaatttt
     9601 attattgcaa tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttagtc
     9661 agaaccgagc gcgctgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa
     9721 actattttac tgataaatat gaattaaccc gcacacactc tgaagtactg gaagcggtga
     9781 aagtggttaa accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt
     9841 acctggcagc caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca
     9901 atgtagagcg cattaaatcc attgaaaatc tggataattt acacacccga gtcgttgatc
     9961 tgaataacct cacatttgat agacagtacg attttattct ttcgactgtg gtactgatgt
    10021 tccttgaggc taaaaccatc ccagggctga ttgccaatat gcaacgttgc actaaacttg
    10081 gtggttacaa cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct
    10141 tcccgtttgc cttcaaagag ggagaattac gtcgatatta cgaaggctgg gagagggtga
    10201 aatacaatga agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac
    10261 tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgc taagaataag gcgcgtgaaa
    10321 attccccacg ctgagatgat ttactgttct tcttttcggt aagcatattt tttatcgaag
    10381 ggatgtgaaa ttaatcacag tagtcgaagt tttttagcag cttaacttac tgaaatttaa
    10441 ctactgatga ttgacttagc ccctttttcg gcattgacta tgtcgtctga aaaggggctg
    10501 aaaaatttat tttcaccaac actttttttg ccacaacacg aagcggcgct ttttgctata
    10561 acttaaaaag taatataatc atctcaggaa actattcatg cgtaccacat catttgcgaa
    10621 agttgcagct ttatgcggct tgttggctct gtctggttgt gcctctaaaa tcacccagcc
    10681 agataaatat tctggttttt taggtaatgc tgccaactta ctgatttagt gtatgatggt
    10741 gatt
//
LOCUS       DAJTOR010000141        10662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTOR010000141 DAJTOR010000000
VERSION     DAJTOR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.141"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            311..1609
                     /gene="kgtP"
                     /locus_tag="OO249_003180"
     CDS             311..1609
                     /gene="kgtP"
                     /locus_tag="OO249_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5637377.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1606..1929)
                     /locus_tag="OO249_003181"
     CDS             complement(1606..1929)
                     /locus_tag="OO249_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5637378.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OO249_003182"
     CDS             complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OO249_003182"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5637379.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYKAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OO249_003183"
     CDS             complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OO249_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5637380.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLHGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVGHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OO249_003184"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OO249_003184"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5637381.1"
                     /translation="MTENTVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREALAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OO249_003185"
     CDS             complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OO249_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5637382.1"
                     /translation="MNTVCTHCQSINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7529..8566
                     /locus_tag="OO249_003186"
     CDS             7529..8566
                     /locus_tag="OO249_003186"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5637383.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAELVQSITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OO249_003187"
     CDS             complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OO249_003187"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5637384.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9608..9991
                     /gene="grcA"
                     /locus_tag="OO249_003188"
     CDS             9608..9991
                     /gene="grcA"
                     /locus_tag="OO249_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5637385.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="OO249_003189"
     CDS             complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="OO249_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5637386.1"
                     /translation="MTPTLLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2653 a         2704 c         2740 g         2565 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgcc tgatgcgacg
       61 ctgcgcgtct tatactccca catatgccag attcagcaac ggatgcggtt tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aatttatcct taagctcctc aataaccatt
      181 ttcctgctaa ctaaattcat ggttaatgtt gcataataat acgtaacaaa tgtataacat
      241 tttctttacc aaaagaaata aacaaaagtg accaacaaaa gcatcggatt acggcaggag
      301 acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac
      361 tcgtcgccgc atttgggcga ttgtgggggc ctcttcaggt aatctggtcg agtggttcga
      421 tttctatgtc tactcgttct gttcactcta cttcgcccac attttcttcc cttccgggaa
      481 cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc tgatgcgccc
      541 aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct
      601 gttatcggtg tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga
      661 aactataggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc agggattatc
      721 cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg
      781 caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac tgctagccct
      841 actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag agtggggatg
      901 gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca
      961 attagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat ctctgaaagg
     1021 attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc
     1261 gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata catcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt
     1561 tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat ccttccagct
     1681 ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca aactgacaga
     1741 gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag
     1981 gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac
     2041 cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga tggtggtatg
     2101 ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agctccagtt gtggatcgtg
     2161 gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt
     2221 gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc
     2281 tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac ggctcaggaa
     2341 aagacgcaga ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc
     2401 ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga cctttttccc
     2461 ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga agtatggcgt
     2521 acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct tgttcaacag
     2581 actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc
     2641 ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat cgttcttgat
     2701 ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat
     2761 attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga taagatgata
     2821 acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct
     2881 ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg
     2941 ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct gcgccatgcg
     3001 gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg
     3061 atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag ctttatacaa
     3121 cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga caatgcaaat
     3181 gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg cgggagcgta
     3241 aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg
     3301 atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag
     3361 gatcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt
     3421 agtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt tagcgtaagc
     3481 ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggctagcgcc
     3541 accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc
     3601 gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt gagatccgag
     3661 cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc acgcgtgacg
     3721 ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat ttcccgatcg
     3781 tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag
     3841 tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag ttgtggctca
     3901 tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct
     3961 tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc ttcaaacggc
     4021 gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga
     4081 atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc
     4141 tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt
     4201 ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg
     4261 acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg
     4321 aaaaccggat cgtgcccaac cacaacccgc aactcctgag cgccagcacg gttagccata
     4381 ctttgcacca acaggccgtg gatccgcgcc tgcggccagg ccatttttac gcgatcgaaa
     4441 atggcgtttg ccgcttgctg gacttcattg gctgtacgca agtacagcat gacgccctga
     4501 acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg ataaccaatc
     4561 tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg gagcgtgttc
     4621 atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg
     4681 gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc
     4741 aacgccggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat atgcataaaa
     4801 gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct gaataaacgt
     4861 cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg
     4921 cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg
     4981 gcgctgggcg aatgaataac catcagcgcg tcaaaatcct ggctgtggag cagaatatcc
     5041 agcgttttaa tatagtgctc actgctggcg tcatcgcgta gatcgagcgg gttagatatt
     5101 gccacatgtt ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc
     5161 agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc agcaccgttg
     5221 ctgataatca tcagccggtc gccatgtagc gggcgcatat ggctaagggt ttccaccgcc
     5281 gaaaacagct cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agccgcatcc
     5341 catgccgggt ccattcctgc cgtggtgttg agcagtcgct gtgccgccgg gctacgtccg
     5401 cttttaatca ccagaatcgg tttattacgc gaggcactac gggccgccga aacaaagcgt
     5461 cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgtgcc
     5521 agatagtcaa gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag
     5581 gaaaagccca tcttacgctg ttgcgcccag tcgaggatgg tgttggagac ggcagccgat
     5641 tgcgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga
     5701 ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat gttatggcgc
     5761 agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt
     5821 ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat
     5881 aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc caacacaccc
     5941 aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc
     6001 aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc aattaccgct
     6061 atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga
     6121 agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac ccagatgttg
     6181 cgttttccct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgctgc
     6241 cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg
     6301 gccttcagcc agggcttcac gcaggcgata ggcagaaagc cgggaaagat cgacggaaat
     6361 gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt ccggccaggt
     6421 accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga tcacttcccg
     6481 ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg ggttttgtac
     6541 cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg
     6601 caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat
     6661 cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt
     6721 ctctggcaaa agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg
     6781 tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gtgttttcgg tcataagaga
     6841 gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa
     6901 gattaaagag attcgttcag ccagctatcg aacggagctt tcggtactgc gccattaagc
     6961 atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag
     7021 cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta
     7081 cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt
     7141 gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag tgtttcaccg
     7201 gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca
     7261 tcttcgatcc gatcgtcggg aatgcgattg atggactgac aatgggtaca aacggtattc
     7321 ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat
     7381 atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc aataggtatg
     7441 atagtcgcag gctttggtac gaaatcatgg agaaccgcgc ggacttcggg taatctgcgc
     7501 gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa
     7561 ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg
     7621 taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc
     7681 ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc cgtggcgcac
     7741 ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg
     7801 taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa cccgcgtcta
     7861 cggcgaaaac gcctgtcagg cactgttcca gagccgtccg gaagcaattg ttcgcgcctg
     7921 gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg
     7981 caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca cggaacatca
     8041 cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag
     8101 ccaggcaggc gcgcaggatt gcgttctggc actggaaaac gaatctaacc cgcataacct
     8161 gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg tgcaggatgc
     8221 ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag agctcgttca
     8281 gtcgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg caggttacac
     8341 cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc tgccagcgaa
     8401 aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa
     8461 cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata tctctgtcgc
     8521 aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa tcagccgggt
     8581 ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg gtaatactgg
     8641 catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg
     8701 gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac
     8761 atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa
     8821 aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag taaacgtctc
     8881 ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca cagtattgag
     8941 cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc gggtgaagcc
     9001 cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa tggcaatgcc
     9061 gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag gatcctggcc
     9121 gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa agacatcttt
     9181 ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt
     9241 aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta attcgttagc
     9301 catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt ctacagaaca
     9361 aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg gtaaaatttc
     9421 acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat cgatgtgtgg
     9481 tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata cactcaagca
     9541 acaatggttt taccaattag ccgcgacagg ctgaacaaat caaataattt tgccggggag
     9601 gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc tgctgaactc
     9661 tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa aagcaggtta
     9721 tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc
     9781 agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct
     9841 gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat
     9901 ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt
     9961 tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag
    10021 agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc
    10081 aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg ctgaaacaga
    10141 tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag caaaacgctg
    10201 acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga cagtgccgta
    10261 acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc ccaaaagctg
    10321 attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt ccacgccagc
    10381 cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg gtcaatcact
    10441 gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc cagactcatc
    10501 cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct aagggcgaga
    10561 atattgttcg gtcttggcgt catagcggta atcagggtgt aagtccaaaa agcacttaaa
    10621 agggtcggtg tcacttgtta tctcctctgt tttggtagac ag
//
LOCUS       DAJTOR010000142        10470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTOR010000142 DAJTOR010000000
VERSION     DAJTOR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.142"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..185
                     /locus_tag="OO249_003190"
     CDS             <1..185
                     /locus_tag="OO249_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5637387.1"
                     /translation="KLGIILQEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITN
                     KCLHNSCIIKICSICY"
     gene            complement(253..1503)
                     /gene="icd"
                     /locus_tag="OO249_003191"
     CDS             complement(253..1503)
                     /gene="icd"
                     /locus_tag="OO249_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5637388.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1675..2328
                     /gene="rluE"
                     /locus_tag="OO249_003192"
     CDS             1675..2328
                     /gene="rluE"
                     /locus_tag="OO249_003192"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5637389.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2338..2799
                     /gene="nudJ"
                     /locus_tag="OO249_003193"
     CDS             2338..2799
                     /gene="nudJ"
                     /locus_tag="OO249_003193"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5637390.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2853..3959
                     /gene="mnmA"
                     /locus_tag="OO249_003194"
     CDS             2853..3959
                     /gene="mnmA"
                     /locus_tag="OO249_003194"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5637391.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3995..4636
                     /gene="hflD"
                     /locus_tag="OO249_003195"
     CDS             3995..4636
                     /gene="hflD"
                     /locus_tag="OO249_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5637392.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4640..6010
                     /gene="purB"
                     /locus_tag="OO249_003196"
     CDS             4640..6010
                     /gene="purB"
                     /locus_tag="OO249_003196"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5637393.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6178..6849
                     /gene="phoP"
                     /locus_tag="OO249_003197"
     CDS             6178..6849
                     /gene="phoP"
                     /locus_tag="OO249_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5637394.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6849..8309
                     /gene="phoQ"
                     /locus_tag="OO249_003198"
     CDS             6849..8309
                     /gene="phoQ"
                     /locus_tag="OO249_003198"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5637395.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8385..9506
                     /gene="roxA"
                     /locus_tag="OO249_003199"
                     /gene_synonym="ycfD"
     CDS             8385..9506
                     /gene="roxA"
                     /locus_tag="OO249_003199"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5637396.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9555..>10470)
                     /gene="pepT"
                     /locus_tag="OO249_003200"
     CDS             complement(9555..>10470)
                     /gene="pepT"
                     /locus_tag="OO249_003200"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5637397.1"
                     /translation="LGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEI
                     MTALAVLQQKNIPHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFE
                     NFNAASVNIKIVGNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLA
                     GMKGTVERADMHYIIRDFDRKQFEARKCKMMEIAKKVGKGLHPDCYIELVIEDSYYNM
                     REKVVEHPHILDIAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHG
                     KHEFVTLEGMEKAVQVIVRIAELTAQRK"
BASE COUNT         2574 a         2680 c         2642 g         2574 t
ORIGIN      
        1 ttaagcttgg tattatcctt caggaaaaag aatatcctat tactatttat aacattgcac
       61 cattcaacaa gaatttatgg agtttactca aaagcagtca agagtgtcct ccaggagaaa
      121 gcaaaataac aaataaatgt ttacataata gttgcattat aaaaatatgc tcgatatgct
      181 attaataata tccacataaa taaaacaacg ggcgtgttat acgcccgttt caatatttaa
      241 ctcatggaga gattacatgt tttcgatgat cgcttcacca aactctgaac atttcagcag
      301 cttagcgcct tccatcagac gttcaaagtc ataagttacg gttttcgcgt tgatagcacc
      361 ttccatacct ttaacaatta aatcagccgc ttcagtccac cccatatggc gcagcatcat
      421 ctcagcggag aggatgatag agcctggatt tactttgtcc tgaccggcgt atttcggcgc
      481 agtaccgtgg gtggcttcaa acagggcgca ttcgtcaccg atgtttgctc caggagcgat
      541 accgataccg ccaacctgcg ctgccagggc gtcagaaatg tagtcaccgt tcaggttcat
      601 acaggcgata acatcatatt cagccggacg cagcaggatc tgttgcagga atgcatcagc
      661 aatcacgtct ttaatgacga tctctttgcc ggtgttcggg tttttaactt tcagccacgg
      721 gccgccgtcg atcagttcac cgccaaactc ttcacgcgcc agctggtagc cccagtcttt
      781 aaacgcgcct tcggtgaact tcatgatgtt gcctttgtgc accagagtca cagagtcacg
      841 atcgttagca attgcgtatt cgatcgctgc acgaaccaga cgtttggtgc cttcttccga
      901 acacggctta ataccgatac cacaatgttc cgggaagcga attttcttca cccccatctc
      961 ttcacgcagg aatttaatca ctttctcggc gtcggcagag tctgctttcc attcgatacc
     1021 cgcataaatg tcttccgagt tttcacggaa gataaccata tcggtcagtt cagggtgttt
     1081 aaccgggctt ggagtgccct gatagtaacg taccggacgc aggcagatgt agagatccag
     1141 ttcctggcgc agggcaacgt tcagagagcg aataccgcca ccaacaggag tggtcagcgg
     1201 acctttaatg gcaacgcgat attcacgaat cagatcaagg gtttcagcag gcagccagac
     1261 atcctgacca taaacctgtg tggatttttc accggtgtaa atttccatcc aggagatttt
     1321 acgctcgcct ttataggctt tctcgactgc agcgtcgacc actttcagca tggctggggt
     1381 tacatctaca ccgattccat caccttcaat gtaagggata atcggatttt caggaacgtt
     1441 gagtttgccg ttttgcaggg tgatcttctt gccttgtgcc ggaacaacta ctttactttc
     1501 cattcacctc tccttcgagc gctactggtt tgctcgtctg ccaccacgtt gcatatgcgt
     1561 ttgcgtcctg cgatacggat gctttagagc aattttttgt taatgatttg taattggctt
     1621 gtcaatacta ccctattgtt tgccgtcatg aaagatgcgc gttattaggc tataatgcgg
     1681 caattcataa tctctgaaaa taccatgcaa aaaacttctt ttagaaatca cctggttaag
     1741 cgattcagct cgcaacgttc taccaggcgt aaacctgaaa accagcccac gcgtgtgatc
     1801 ctgttcaata aaccctacga tgttcttccg cagttcaccg atgaagccgg acgcaaaaca
     1861 ttaaaagaat tcatcccggt tcagggcgtt tatgcagcag gtcgtcttga ccgcgatagc
     1921 gaagggttac tggtgctgac caataacggc gcgttgcagg cgcgtttaac ccagccgggc
     1981 aaacgcaccg ggaaaatcta ttatgtgcag gtggaaggta ttcccacaca agacgcactt
     2041 gaagccttgc gcaatggcgt aaccttaaat gatggcccta ccctgcccgc cggcgcggag
     2101 ctggttgacg aaccagcgtg gttatggccg cggaatccac caattcgtga acgcaaaagt
     2161 attcccacca gctggctgaa gatcacctta tatgaaggac gtaatcgcca ggtgcgccgc
     2221 atgaccgccc atgttggttt ccccacgctg cgactgattc gttatgcgat gggtgattac
     2281 actttggata accttgccaa tggcgaatgg cgagaagcaa cagaataagg atgcacaatg
     2341 tttaaaccac acgttaccgt tgcttgcgta gtgcacgcag agggtaaatt tttagtcgtt
     2401 gaagagacga ttaatggtaa agcgttatgg aaccaacctg ccgggcatct ggaagccgat
     2461 gaaaccttag tggaagctgc cgcccgtgag ctatgggaag aaaccggcat cagcgcgcaa
     2521 ccgcaacact ttattcgtat gcatcagtgg attgcgccag ataaaacgcc gtttttgcgt
     2581 ttcctctttg ccattgagct tgagcaaata tgcccgaccc agcctcatga cagcgatatc
     2641 gactgctgtc gttgggtcag cgccgaagaa attttaaagg cgtcaaatct tcgttcgccg
     2701 ctggtggcgg aaagtatccg ttgttatcaa agcgggcaac gctatccgct ggagatgatt
     2761 ggcgatttta actggccttt tacaaagggt gtcatctaaa aggctgcgtg atagaatacg
     2821 ccgccttgaa gttcaatgtc gtgagtgatc caatgtctga aaccgcaaaa aaagtaatcg
     2881 tcggcatgtc cggcggtgtc gattcctccg tttctgcctg gctgttgcaa caacagggat
     2941 atcaggttga aggcctgttt atgaagaact gggaagaaga cgacggtgag gaatattgca
     3001 cagcggcagc ggatctggct gatgcccaag ctgtctgcga caagctcggc attgaactgc
     3061 acaccgttaa ctttgctgcc gagtactggg acaacgtctt cgaactgttc cttgccgaat
     3121 ataaagccgg tcgcacgccg aatccggata ttctgtgcaa caaagagatc aaatttaaag
     3181 ctttcctcga atttgccgcc gaagatttag gtgccgatta tatcgctacc ggtcattacg
     3241 tacgtcgggc cgatgtcgat ggcaagagcc gcctgctgcg tggtctggac agcaataaag
     3301 accagagcta cttcctttat acgctcagcc atgagcagat tgcgcaaagc ctgttcccgg
     3361 tcggcgaact ggaaaaaccg caggtgcgta agattgctga agatcttggt ctggtcaccg
     3421 cgaagaaaaa agactctacc ggcatttgct tcattggcga acgtaaattc cgcgagttcc
     3481 tgggccgtta tctcccggcg caaccgggca aaatcattac cgtcgatggc gatgaaattg
     3541 gtgagcacca ggggctgatg tatcacactc tcggtcagcg taaaggtctg ggtatcggtg
     3601 gcaccaaaga aggcaccgaa gaaccgtggt atgtggtgga caaagacgtc gaaaacaaca
     3661 ttctggttgt tgcgcagggc catgaacacc cgcggctgat gtctgtcggg ttgattgccc
     3721 agcagttgca ctgggtcgat cgcgaaccat tcaccggtac tatgcgttgc acggtaaaaa
     3781 cccgctatcg ccagaccgat atcccttgca ccgtcaaggc gctggacgat gatcgcattg
     3841 aagtgatttt cgatgaaccg gttgccgccg tgacgccggg ccagtctgcc gtcttctata
     3901 acggtgaagt gtgcctcggt ggcggtatta ttgagcagcg tctgccgctg ccggtctgat
     3961 tattatcttt acttaaaaca aggaagcagt gaacgtggca aagaattact atgacatcac
     4021 cctcgccctg gccggtattt gtcagtcggc acgcctggtg caacaactcg ctcaccaggg
     4081 gcattgtgat gccgatgcgc tacacgtctc actcaacagt attattgata tgaaccccag
     4141 ctcgacgctg gcggtttttg gcggtagcga agccaacctg cgcgtcgggc tggaaaccct
     4201 gctcggcgtg ctcaatgcca gcagtcgcca gggcttaaac gccgaattaa cccgctacac
     4261 actcagcttg atggtgcttg agcgcaaact ctcctcagcg aaaggcgcgc tcgacactct
     4321 gggcaaccgg atcaacggcc tgcaacgcca gctcgaacac ttcgatttac agtccgaaac
     4381 gctgatgagc gcgatggctg ctatctatgt tgatgtgatc agcccgcttg gcccgcgcat
     4441 tcaggtcacc ggttcccctg ctgtactgca aagcccacaa gtgccggcga aagttcgcgc
     4501 aaccctgctg gcaggcattc gcgccgccgt gctctggcac caggtcggcg gcggacgtct
     4561 gcaactgatg ttttctcgta atcgcctgac cactcaggca aaacaaattc ttgctcattt
     4621 aaccccggag ttgtgatcta tggaattatc ctcactgacc gccgtttccc ctgtcgatgg
     4681 acgctacggc gataaagtca gcgcgctgcg cgggattttc agcgaatatg gtttgctgaa
     4741 attccgtgta caagttgaag tacgttggct gcaaaaactg gccgcgcacg cagcgatcaa
     4801 ggaagttcct gcttttgctg ccgacgcaat cggttacctt gatgcaattg tcgccaattt
     4861 cagcgaagaa gatgccgcac gcatcaaaac catcgagcgt accactaacc acgacgttaa
     4921 agcggttgag tatttcctga aagaaaaagt ggcggagatc ccggaactgc acgcggtttc
     4981 tgaattcatc cactttgcct gtacttcgga agatatcaat aacctctccc acgcattaat
     5041 gctgaaaacc gcgcgtgatg aagtgatcct gccgtactgg cgtcaactga ttgatggcat
     5101 taaagatctc gccgttcagt accgcgatat cccgctgctg tctcgtaccc acggtcagcc
     5161 agccacgccg tcaaccatcg gtaaagagat ggcaaacgtc gcctaccgta tggagcgcca
     5221 gtaccgccag cttaaccagg tggagatcct cggcaaaatc aacggcgcgg tcggtaacta
     5281 taacgcccac atcgccgctt acccggaagt tgactggcat cagttcagcg aagagttcgt
     5341 cacctcgctg ggtattcagt ggaacccgta cactacccag attgaaccgc acgactacat
     5401 tgccgaactg tttgattgcg ttgcgcgctt caacaccatt ctgatcgact ttgaccgtga
     5461 cgtctggggt tatatcgccc ttaaccactt caaacagaaa accattgctg gtgagattgg
     5521 ttcttccacc atgccgcata aagttaaccc gatcgacttc gagaactccg aagggaatct
     5581 gggcctttcc aacgcggtat tacagcatct ggcaagcaaa ctgccggttt cccgctggca
     5641 gcgtgacctg accgactcca ccgtgctgcg taacctcggc gtgggtatcg gttatgcgct
     5701 gattgcgtat caatccaccc tgaaaggcgt gagcaaactg gaagtgaacc gtgaccatct
     5761 gctggatgag ctggatcaca actgggaagt gctggctgag ccaatccaga cagttatgcg
     5821 tcgctatggc atcgaaaaac cgtacgagaa gctgaaagag ctgactcgtg gtaagcgcgt
     5881 tgacgccgaa ggcatgaagc agtttatcga cggtctggcg ttgccagaag aagagaaagc
     5941 ccgcctgaaa gcgatgacgc cggcaaacta cattggtcgc gccatcacca tggttgatga
     6001 gctgaaataa actcgtatca gtgccggatg gcgatgctgt ccggcctgct tattaagatt
     6061 atccgctttt tattttttca ctttacctcc cctccccgct ggtttattta atgtttaccc
     6121 ccataaccac ataatcgcgt tacactattt taataattaa gacagggaga aataaaaatg
     6181 cgcgtactgg ttgttgaaga caatgcgttg ttacgtcacc accttaaagt tcagattcag
     6241 gatgctggtc atcaggtcga tgatgcagaa gatgccaaag aagccgatta ttatctcaat
     6301 gaacatttac cggatattgc gattgtcgat ctcggattgc cagacgagga cggtctgtca
     6361 ctgattcgcc gctggcgtag caatgatgtt tcactgccga ttctggtatt aaccgcccgt
     6421 gaaagctggc aggacaaagt cgaagtatta agtgctggtg ctgatgatta cgtgactaaa
     6481 ccatttcata ttgaagaggt gatggcgcga atgcaggcat taatgcggcg taatagcggt
     6541 ctggcttcac aggtcatttc gctacctccg tttcaggttg atctctctcg ccgtgaatta
     6601 tctattaatg acgaagtgat caaactgacc gcgttcgaat acaccattat ggaaacgttg
     6661 atacgcaata atggcaaagt ggtgagcaaa gattcgttaa tgctccaact ctatccggat
     6721 gcggagctgc gggaaagcca taccattgat gtactgatgg gacgtctgcg caaaaaaatt
     6781 caggcacaat atccgcaaga agtgattact accgttcgcg gccagggcta tttgttcgaa
     6841 ttgcgctgat gaaaaaatta ctgcgtcttt ttttcccgct ctcgctgcgg gtacgttttc
     6901 tgttggcaac ggctgcggta gtactggtgc tttcgcttgc ctacggaatg gtcgcgctga
     6961 tcggttatag cgtcagtttc gataaaacta cgtttcggct gttacgtggc gagagcaatc
     7021 tgttctatac ccttgcgaag tgggaaaaca ataagttgca tgtcgagtta cccgaaaata
     7081 tcgacaagca aagccccacc atgacgctaa tttatgatga gaacgggcag cttttatggg
     7141 cgcaacgtga cgtgccctgg ctgatgaaga tgatccagcc tgactggctg aaatcgaatg
     7201 gttttcatga aattgaagcg gatgttaacg ataccagcct cttgctgagt ggagatcatt
     7261 cgatacagca acagttgcag gaagtgcggg aagatgatga cgacgcggag atgacccact
     7321 cggtggcagt aaacgtctac ccggcaacat cgcggatgcc aaaattaacc attgtggtgg
     7381 tggataccat tccggtggag ctaaaaagtt cctatatggt ctggagctgg tttatctatg
     7441 tgctctcagc caatctgctg ttagtgatcc cgctgctgtg ggtcgccgcc tggtggagtt
     7501 tacgccccat cgaagccctg gcaaaagaag tccgcgaact ggaagaacat aaccgcgaat
     7561 tgctcaatcc agccacaacg cgagaactga ccagtctggt acgaaacctg aaccgattgt
     7621 taaaaagtga acgcgaacgt tacgacaaat accgtacgac gctcaccgac ctgacccata
     7681 gtctgaaaac gccactggcg gtgctgcaaa gtacgctgcg ttctctgcgt agtgaaaaga
     7741 tgagcgtcag tgatgctgag ccggtaatgc tggagcaaat cagccgcatt tcacagcaaa
     7801 ttggctacta cctgcatcgt gccagtatgc gcggcgggac attgctcagc cgcgagctgc
     7861 atccggtcgc cccactgctg gacaatctca cctcagcgct gaacaaagtg tatcaacgca
     7921 aaggggtcaa tatctctctc gatatttcgc cagagatcag ctttgtcggt gagcagaacg
     7981 attttgtcga ggtgatgggc aacgtgctgg ataatgcctg taaatattgc ctcgagtttg
     8041 tcgaaatttc tgcaaggcaa accgacgagc atctctatat tgtggtcgag gatgatggcc
     8101 ccggtattcc attaagcaag cgagaggtca ttttcgaccg tggtcaacgg gttgatactt
     8161 tacgccctgg gcaaggtgta gggctggcgg tagcccgcga aatcaccgag caatatgagg
     8221 gtaaaatcgt cgccggagag agcatgctgg gcggtgcgcg gatggaggtg atttttggtc
     8281 gccagcattc tgcgccgaaa gatgaataaa tatgtccata cttcacgcat tacgttaagc
     8341 atccgttata atcggttgca gataccagcc tgtggatgct taacatggaa taccaactca
     8401 ctcttaactg gcccgatttt cttgaacgtc actggcagaa acgcccggtg gtgttaaaac
     8461 gcggctttaa taattttatt gacccgatct ctccagacga gttggcgggt ctggcgatgg
     8521 aaagcgaagt tgacagtcga ctggtcagtc accaggatgg caaatggcag gtcagccacg
     8581 gcccgttcga aagctacgat catctcggtg aaaccaactg gtcattactg gtacaggcag
     8641 tgaaccactg gcatgagccg accgccgcgc tgatgcgacc gttccgtgaa ctaccggact
     8701 ggcgtattga tgatctgatg atttcttttt ctgtacctgg cggcggcgtc ggcccgcatc
     8761 tcgatcagta cgacgtgttt atcattcagg gtaccggacg tcgtcgctgg cgagtgggcg
     8821 aaaagctgca aatgaaacag cactgcccac atccggatct gttacaggtc gatccgttcg
     8881 aagccatcat cgatgaagag ctggagcctg gtgatattct ttatattccg ccaggattcc
     8941 cgcatgaagg ctacgcgctg gaaaatgcga tgaactattc cgtgggcttt cgcgcgccaa
     9001 atacgcggga actgattagt ggatttgccg attatgtgct gcaacgtgaa ctgggcggca
     9061 actactacag cgatcctgat gttccacctc gcgctcatcc tgcggatgtt ctgcctcaag
     9121 agatggataa actgcgtgag atgatgctcg aattgatcaa ccagccggaa cactttaagc
     9181 aatggtttgg cgagtttata tcccagtcac gtcatgaact ggatatcgcg ccgccggaac
     9241 cgccttatca gccagatgaa atctacgatg cgctgaaaca aggtgatgtg ctggtgcgcc
     9301 tgggtggtct gcgcgtattg cgcattggcg acgacgtgta tgccaatggt gagaagatcg
     9361 attccccgca ccgtccggca ctggatgcac tcgccagcaa cattgcgctg actgcggaga
     9421 attttggcga tgcgctggaa gatccgtcat tcctcgcgat gctcgcggcg ctggtcaata
     9481 gcgggtattg gttcttcgaa gggtaagttt ggatttaagc cggatgcggc atcacatgcc
     9541 gcatcccttt tcgcttactt ccgttgcgcc gttaactcgg caatacggac gatcacctgc
     9601 accgcttttt ccataccttc cagagtcaca aactcatgct taccatgata gttgtaaccg
     9661 ccagtgaaca agttcgggca cggtaatccc ataaacgaca actgcgcgcc gtcggtaccg
     9721 ccgcggatcg gtttcagttc cggttcaata tcgcagtcac gcattgcctg ctgggcgata
     9781 tcgagaatat gcggatgctc aaccactttc tcgcgcatat tgtagtaact gtcttcaatc
     9841 accagttcaa tgtagcaatc aggatgtaac cctttgccca cttttttggc gatctccatc
     9901 attttacatt tacgcgcttc aaactgttta cggtcgaaat cacggatgat gtagtgcata
     9961 tcggcccgtt caacggtgcc tttcatgccc gccaggtgat agaaaccttc atagccttct
    10021 gtcatttccg ggctttcatc cgccggaact tccgcatgaa tacgtgccgc cagcgacagc
    10081 gcatttacca tcactccttt cgccgtgccc ggatgaacat tgttaccgac aattttgata
    10141 ttgaccgatg cggcgttgaa gttttcaaac tccagttcgc ctacgccacc accgtcaaca
    10201 gtgtaagccc agcgggcatc gaaggcatca acatcaaaat gtttcgcccc tttgcccact
    10261 tcttcatccg gggtaaaggc gacgcgaata tcaccatgcg gaatgttttt ctgttgcaat
    10321 accgccagcg cggtcatgat ttctgcaata cctgctttgt catcggcacc taacaaggtt
    10381 ttaccatcgg tggtaatcaa tgtctgaccc agtagctgat gcagcaccgg gaacataacc
    10441 ggtgataaaa cttcatcgcc gatacccagc
//
LOCUS       DAJTOR010000143        10322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTOR010000143 DAJTOR010000000
VERSION     DAJTOR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.143"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..429
                     /locus_tag="OO249_003201"
                     /pseudo
     CDS             1..429
                     /locus_tag="OO249_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            512..769
                     /locus_tag="OO249_003202"
     CDS             512..769
                     /locus_tag="OO249_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637398.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1327..2094)
                     /gene="fecE"
                     /locus_tag="OO249_003203"
     CDS             complement(1327..2094)
                     /gene="fecE"
                     /locus_tag="OO249_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR5637399.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2095..3051)
                     /gene="fecD"
                     /locus_tag="OO249_003204"
     CDS             complement(2095..3051)
                     /gene="fecD"
                     /locus_tag="OO249_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR5637400.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3048..4046)
                     /gene="fecC"
                     /locus_tag="OO249_003205"
     CDS             complement(3048..4046)
                     /gene="fecC"
                     /locus_tag="OO249_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR5637401.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4043..4945)
                     /gene="fecB"
                     /locus_tag="OO249_003206"
     CDS             complement(4043..4945)
                     /gene="fecB"
                     /locus_tag="OO249_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR5637402.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4990..7314)
                     /gene="fecA"
                     /locus_tag="OO249_003207"
     CDS             complement(4990..7314)
                     /gene="fecA"
                     /locus_tag="OO249_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR5637403.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7401..8354)
                     /gene="fecR"
                     /locus_tag="OO249_003208"
     CDS             complement(7401..8354)
                     /gene="fecR"
                     /locus_tag="OO249_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR5637404.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8351..8872)
                     /gene="fecI"
                     /locus_tag="OO249_003209"
     CDS             complement(8351..8872)
                     /gene="fecI"
                     /locus_tag="OO249_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR5637405.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9170..9352
                     /locus_tag="OO249_003210"
                     /pseudo
     CDS             9170..9352
                     /locus_tag="OO249_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9346..9450
                     /locus_tag="OO249_003211"
                     /pseudo
     CDS             9346..9450
                     /locus_tag="OO249_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9790..10047
                     /locus_tag="OO249_003212"
     CDS             9790..10047
                     /locus_tag="OO249_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR5637406.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2409 a         2742 c         2831 g         2340 t
ORIGIN      
        1 cgcaagaaaa ccagtgggct gggccatctc gttctcgccg gacagcaggc ttaccatgaa
       61 agcactggaa atggcatggg aaacccgtgg taagcccgtc ggggtgatgt tccaagcgat
      121 caaggcagtc attatacgag caggcagttc cggcagttac tgtggcgata ccagatcaga
      181 cagagtatga gccggcgtgg aaactgctgg gataacagcc caatggagcg cttcttcagg
      241 agtctgaaga actaatgggt gccagcgacg ggctatgtaa gcttcagcga tgcagctcac
      301 gcaataacgg actatatcgt tggatattac agcgcactaa gaccgcacga atataatggt
      361 gggttaccac caaacgaatc agaaaaccga tactggaaaa actctaacgc ggaggccagt
      421 tttagttgac cacaacagac tacctgaagg gagccgcggt cgcctagcag ttgcagtagc
      481 aggagatcat ccagccgcag tacaggtcac gatgactctg gttaatgata ccggctttga
      541 ccccgtattt tccggctcta tcgctgaatc atggcgtcag cagccgtgca caccatccta
      601 ttgttgtgac tgggaggctg ccaccatgct tcgcgctttc cctctggcga aaaagggaga
      661 aggacgggcc cgtctgcctt cactttatgc cagcttcggt aagctgggtg agacaccgac
      721 tcatgaagat atcattgata acaatcgatc catcaactgg cctgtataac gtggctgccg
      781 gtgattaaga aagctgcacc tacctaagta gtagcaaacg cacacttttt agaaaaatcg
      841 atggtcagaa actggattag caattccgtt ccagggttgc ttttgattta cgttggcgtc
      901 tgatcattga tttatcctca aaagcccaac ctcattggta atgaaccagc tccgtgaatg
      961 tccgctctgg cacagagcga aattttttga tctccccccc tgaaatctaa acttagtcat
     1021 gtcacgtttt tgggtttcta aaattttaac ttcgcgtttt tcgttgccgt aagggttata
     1081 cagaaatgtc cgttaagcag agttcaaaat tgattgccgt gatcacgact ggtttgaaag
     1141 ccgcgcccaa gcctgtacag ctctggtttg cgttgattat gaacctgtca gcctaaagca
     1201 agcggatgga cgatgagtat tggtaatctt tcagagtccg gaaaagttca gccccagtct
     1261 gaacaggctt gctggcgcca gtccagtttc attcagtcgt ggtttggttc ttacggcctg
     1321 tgcaatctac ctcattaggc acatcggcct gccagatacc ggctcggggt gtatttccgc
     1381 ttccacgctg aatactgttc tcagcaatcc tggggtcatc acctcttctg gtgtgccttg
     1441 cgccataaca tgtccgtttg ccattaccac cagttgatcg cagtaccggc tagcctgatt
     1501 aaggtcgtgc agcacagcga ccaccgtttt cccctgagtc cggagttcgc ccatcaaccg
     1561 catcaggtcc acctggtgat tgatatcaag ataggtggtt ggctcatcaa gtaatacaac
     1621 gggcgtattc tgggccagga ccatcgccag aaatgcgcgc tggcgctgac cgccggaaag
     1681 ctcggttaac cgacgaacgg caagatgatt gatccgggtc tggttcatgg cgacattaac
     1741 tcgtgcattg tcttcagcgg agagacgccc ccagagtgac agccagggat tacgaccata
     1801 cgaaaccagc tcctggactg tgatcccctc tggcgttaaa tggtgctgag gcagcagcga
     1861 aagcctgcgg gccaactggc gtgatgagag catatttatg ggattatcgc cgagaaatac
     1921 ggtgccagac tgcggcatta aaagccgcga aaaacagttt aacagcgtcg atttcccgca
     1981 accgttaggg ccgatcaggg cggtgatctt ccccgttggc agtgagagtg aaacgtcgtt
     2041 aagtaccttg tctgtcccgt aactgaccgt cagattttca gttcgtaaag tcatttatcg
     2101 cattctcaca agcaaccaga caaaccacgg cgcaccgata atggcggtca gcacgccaac
     2161 cgggagctcc agtgggggat gaataattct cgccagcaga tcggcaacca ccaacagcaa
     2221 cgcacctgtc agggctgaaa caggcagcag tctgcggtga cgtccaccgg tgatgctacg
     2281 catcatatgc ggcaccacga gaccaataaa gctaatcggg ccgcaggcgg ccacgccggt
     2341 agatgtcatg gcgacagcta gtaacaaagc ccagaatcgg gtatggggca ccgacacacc
     2401 gagcgtggtg gcgcgcgcat cgccgagtgc aaggaggtcg agatcgcggc aaaaactcag
     2461 gctcagcggc agaaataaaa tcatcagcgg gatggcaatc ttcacaaagc tccagtcacg
     2521 gccccataag ctgccggtca gccacagcag ggcgttgttc acatcctgcg ggcgcgagag
     2581 catcagataa tccgtcaggc tggcccagca tgcagaaagc gccacgccgg tgagcgccag
     2641 cttcatcggc tggtgggtct ttgccagcat cttcagtaat atcaaccccg ccatgccgcc
     2701 cgcaaaggcc agcagcggca gcaccatcac gggcagtgac ggcataagaa gtagagcccc
     2761 cacagaggcc aggctggcgg catggttaac gccgagaata tccggtgatg ccagagggtt
     2821 gcgcacaatc ccctgtatca gcacgcccgc cacggcgagg gctgcaccga caaacagtgc
     2881 cagcagcaag cgcggcagtc ggtactccat caatacataa taatgctcgc gtccggcctg
     2941 ccagtcggtc agcagcgcgc gccacggcac ggggatcact cccatatgga gtgataacag
     3001 cgcacagccc gccagggcaa gggtgatgaa aataaccagc gcaattttca tcctcgcctc
     3061 ctcacaagcc agacaaagca agggctgcca atcagcgcca gcactgcgcc tgcgggcaga
     3121 tctccgggga aggccagcgc gcgtgcgagt acatctgcca gcagcatcag cgtggccccc
     3181 agcagcatgc tcactggcag tacgttgcgc tgatcgaagc ctgcccagaa gcgcgccaga
     3241 tgtggcacca gcagaccgat aaacgccacc ggacctgcca cgctgacgca cgcaccaacc
     3301 agaagcagca ctaacatatt gatgaccaaa cgtagcctcg tcaggttcac tcccagcgta
     3361 tgggcggtgc tgtcgctgag gttgagcagg ttcagttgat tcgccagcag taacacgaca
     3421 gggactgcag tgaccaccac cggcaagagc tgccagacat cctgccagcg ggcgtgggac
     3481 actccgcctg ccagccagta aaagatgccg taagcatgat cttcggccag cagcagggtg
     3541 atgcgggtaa ggcccataca aaaggccgac agcgcgatac ccgcgaggat cagtttgttt
     3601 ctgtcatggg tatgacgaaa tccgcctcct gcggtcatga ccagcagcca gctcacgccg
     3661 cccccgcatg ccgcgatgaa cgacagagaa tagcctgcaa tcggcgtcgg actcagcgcg
     3721 ctggtaagcg ccatagccag cgccgcgccg ctgttaatgc cgagcagtga aggagaggcc
     3781 attgggttgt gggtcagggt ttgcagcagc gtgcccgcga gcgccaggct tgcgccgatc
     3841 agaacggcga ccaggcttcg tggcaaacga aggttttgca ccagcgcttc tggtagcgtt
     3901 ggcgtgtgtc caggcagcag ggcgcgggtt gcatctgctc cggaaacagg aatggccgag
     3961 tagcaaaaca gactcagcca gaaaataata ataagtgctg caacgggaag cccccacagc
     4021 agcatcgggt gttttatcgc ggtcatttca caacggtaag cggctgatgg tggaagattt
     4081 ttaccgtgtc agcggcaata cgctctgcag caaaaatacc gcgcatccgc gcccaggtgt
     4141 tactgtcgac cgaagcaacc tgctgcttct gcgcggcggt taacatctgc cagagcggat
     4201 cttgttgcca gcgtttaaca atgctctctt cgcgatagtg ggcaaccagc agccaggcag
     4261 gattgaccgc cagcagttgc tccaggccga tggacggcat ggacgaaccc gccatcgcag
     4321 cgggaacgtt cagccccaga gaggccagca cgctgccggt ccaggtctcc tgagtatgca
     4381 ggttgaattg ctgttcgcgt gatgtgccaa aggccacgcg tgtccctttg ggaagctggc
     4441 tggcccactg cgccatcctc tctttatgtt gttccagacg tgcctgcatc tctcgctttt
     4501 tacccaccat ttcgccgatg atagccgcag attgcaaatt ttcagcgtag gtttcgttgc
     4561 gggacttaag cagcagtacc ggcgcgattt gctgcaaggc gatgtaaacc cccgcatggc
     4621 gactgctgtc ggcaatgatc aggtctggtt tcagagcggc aatggcttcc aggctcggct
     4681 gcgcgcgcgt tccgacggac tgccacggtt tcaggtgcgc acgcacttcg ggcaggatgc
     4741 gttttgcatc gttatcgtcg gcaataccga tcgggctgac gtccacggcg gccagcgcat
     4801 cggcgaacga gagttccagc accacaatcc gttgtggcgt tttttcgagt gtaaacgtgc
     4861 cgtgttcgtc ctgaaccatg gcggcaaagg cgtggctgat caccagcagc aggcctgcaa
     4921 aaagaaaacg gataaatgcc aacataatca cattccagct aaaagcccgg caagccgggc
     4981 gttaacacat cagaacttca acgacccctg catatacagc gtgcgcggct ggcctgcata
     5041 gatgcctttg ttgttgtcgt cataagagcg gatgaagtag tcctggtcga agatgttttt
     5101 cacaccgaat gccaggttca gatctgccat ctgcgggcca aagtcatacg ccacgcgtgc
     5161 accccagagc atgaagccgg gaatgcggcc ggtactgccg tcggcgctct ctttcaccgt
     5221 attggcgtta tccgcaaact ggctggactg gaaatcgctg ttcagattga acgtccagtt
     5281 tcctggcttg tagtccacgc ccagcgtgcc tttatgtttc ggggagaatg gtaccagatt
     5341 gccgtaggtg tcgcctttct cgcggatttc cgcgttcaca tacgcatagc tggcgtagat
     5401 ggaaacgtta tcaagcgttg gcgttagcgt acccagatcg taacgtgcct gcgtttccag
     5461 cccggtatgg cgcgttttgc cacgtgcagt gacggtgtcg ttggtctggt tggagtcgta
     5521 ctgattgtta aagttaatca ggaacagccc catttccgcc gtcagcgcgc cgtcgtcgta
     5581 gcgggtaccg agttcccagg ttcgcgcttt ttccggttcc acattgccgc tttgcacagc
     5641 cttgccaatc tggctgtact gtacggtgcc gaacgagcct tcagtgtttg cataaagatt
     5701 ccagctgtca gtcaggtgat agagcacgtt caacgccgga agcggtgcgt tatagctcac
     5761 ttcttcgtgc gtgcctgtga tggcgttgtt ctggtatgac tcgatatgtt cgaaacgcat
     5821 acccggcgtg atggtccagt tgccgatgtc gattttgtca tccagatacc aggcgtgcgc
     5881 ctcggtgccg gaacgcgtat cgcggtcgta agggcttgag ccggacggca actgcccgct
     5941 gctggtggcg gtgtagtaac gcatttcatg cgttgattca ttcacatagc gatagcccac
     6001 gcccacttcg tgcgcggaag ggccgatcat aaagatctgg ctgtagcgtg gctcaatacc
     6061 gcgcacccag tagttacgcg gcgagagggt gatgcgtttg ccttgctcca ggtagccgct
     6121 gcgcagggtt tgggtgtaga acccctgaat gttgaattta tgctggctgt ctggctggaa
     6181 ctggtagccc aggctcgcca gcttgcgacg accccagaag cggtcatacg ggcgggtgga
     6241 ttgccagcga tcggcgtcgt aatccgcgcg agacaggcca ccgggcatgt cggcttcacc
     6301 gtcgtaatat tgcagcaggc tgttgaaggt gtgcacctca tccggcgcat atttgctttt
     6361 cagcatcagg tcgtcgatgc gggtggcgct gtgctcgcgc cagtcactgc cgcgcgtgcc
     6421 ggagtagagc agcgcggtgc caaaaccgtt gtccgctgtg ccgcccacca tcaggttgtg
     6481 cgtctctttc gggttgtttt gtgaagaggt tgggctgagc tgaccttcca cgcccgcctc
     6541 gataccaaag tcctgcggaa tggcacgggt aacaaagttc accacgccgc ccacgctctg
     6601 cggtccgtaa cgcaccgcac caccaccgcg taccacgtca atggcatcca tgttgccgag
     6661 cgaaatggga gccagtgaaa gctgcggctg accgtaaggg gcgaagggga cggggatgcc
     6721 gtccatcagg acggtcgagc ggctggcgag gcgcgggttc aggccccgga tgccaaagtt
     6781 catcgccagg tcgtggctgc cggtgccgtt gttttccggc gcgctgacgc cagggatgcg
     6841 gttaagtacc tcacgcatgg tggttgcgcc ggttttggcg aaatcctcac ggcggatcac
     6901 gtcacgcgcg ccagcatgtt caaatacgtc gttttcacgc gcatcaccca gccagtcgcc
     6961 gaccacggtc agggcatctt cttttggtgc gggcgcgggc tccagcgtcc agctgttatt
     7021 tcccagcggt tttacctgca gtccgctgcc gtccagcagt tgttgcaggc cgctctcgac
     7081 gtcgtaatcg ccgtgcaggc cgttgctctg cttgccgcgc gtcaggctgg cgtcaaccga
     7141 gagggtaaat ccgctgtgtg cggcatactg attgagcgct ttatcgagcg atcccggtgc
     7201 gatattaacc tgtgcagcaa aagcggaaaa cgagagaccg gccagcggca gcaggctcag
     7261 gcgaatggcg ttaaccaaag gtgttgtttt acgaaaaacg cgtaacggcg tcataccttc
     7321 cccatcatca tttttgttgt gttcagctat gagtcgaacg agaagggtaa aaaggacaat
     7381 cgaaataaga attattttcc ttacagtggt gaaatgttta tccagtaccg cgtaatagac
     7441 tgaattttaa cgggaagcgt ttgcgcgata acgttcagga tcgcatcggt atttttcagc
     7501 gggaacgtcc cgctcaggcg cagcccggca acggcgggat cgcagcgcag cacgccgttg
     7561 cggtaacggg ttagcgtggc tatcacctca cccagcggtt tatcgctgaa gctcaggatg
     7621 tccttcgtcc agcttgtact ctcgtcatcc agcggtttca ctgcgccaaa ctcagaggcg
     7681 ctgaactgca ggctttcacc agcgttcacg atgcgttttt gcgcgggggc actggcgaga
     7741 agcacttcca cagcgtgctg ctgaacgtca agctgcgtga aattatcctg ctggcggacg
     7801 gtaaattctg tccctaaagc agtgagctgg ccctgacggg tcaggacacg gaaggggcgt
     7861 tgcagggcat ctttcgcggt agtaatggcg atttcaccgt accagagccg gacggtgcgc
     7921 tgatgcgcat caaaacgcac atccgccgcg ctttgggtat tcagcgtgag cagggagcca
     7981 tcttccagtt gctgacggct gactgtgcct ttggcggtgc ggtaatctgc ccgcagacct
     8041 tcgccggttt ccgactgcca gagctgccag cctccgccag cgccgagcaa cagcagtaat
     8101 cctttcatca cgtgacggcg ggtgaggcgg gtatcgtgca acgcccggct ggcaacgtcg
     8161 ccaggcacac cgccaagctg gttgcgcagg ttttcaacct gctgccaggc ccactggtta
     8221 tcctgatcct gttcatacca ctgttgccag cgcgcttcct gttgtgggct gacgcgctcg
     8281 ccgcttagca cggcatacca gtgggaagct gaacgcagcg cctgacggcg ggaatcggtt
     8341 aacaaaggat tcataaccca tactccagac ggaacagcag gcagtgctcg acggctttcg
     8401 ccacgtattt tttcacggag ctgatggaaa caccgagttt gtgcgcaatc tcgctgtatg
     8461 tcagaccatc cagttgcgaa agcagaaacg cttcacgtgt tttgccgttt agcccgtcca
     8521 gcatgctgtc gaggagttgt agggtctcga gttggctttc gcgttcctca ggtgaaggcg
     8581 ctcccccctc cggcataagc gccagcatct ccagatacgc tttttccagc gcgtttcggc
     8641 gaaacaggtc caccatcacg cgtttggcga tagtgcagag gaaggagcga ggatcgcgga
     8701 tcgtcgagag cgtttcgctg accattaccc gcaaaaaagt gtcctgggca atgtcatctg
     8761 catcaaaagc agactggagt ttgcgcgtca gccagctttt caaccagccg tgatgtgtgc
     8821 cataaagcga ctcgaacgtt aaggaagctg tggtagtggc gcggtcagac atgcggagtg
     8881 catcaaaagt taattatcac gtagtcatat taatatgaga atggttatca ttacaattgg
     8941 aaataaaatt gtttccaata gacattttta acatgttgtt tttctaagtg ttataaggta
     9001 ggtataaaat gggatggagc ctctgcttct gacatgtgtc ggtcagaatg actcatgatg
     9061 tggtctgcta ttattgacat cctcactgcc ctaaaggatg gggatttcgg taatgatctt
     9121 acccagcaat agtggacacg cggctaagtg agtaaactct cagtcagagg tgactcacaa
     9181 gacaaaacag tttcaaccag taaaaaaacg taaacaccat tcgcatgaat ttcgtagtta
     9241 agatctgaag cttgcagaat gcatcggtgt tactgccgga gtccgtgaac ttagtctgca
     9301 tgaatcacca cagctttaca tctgtcgcag taaacagcaa aatcagcaaa cttacttaat
     9361 tatatcgagt gcgattacaa tcgctggcga tggtacagtt ggtgtggcgg tctcagtccg
     9421 gaacaatttg aaaatcataa tctcacttaa aacggcatcc attttacaaa agatggccgt
     9481 ctgtgtattt gtaatgaata ccttctacat gtttagagtg cataatccac gacaaggcga
     9541 tatcctaaaa attagctagc aggcaacttt gatggcatag ataatgtttt tagtaaaaaa
     9601 gagttgactg aatataatac agcctcttcc tacataagaa ggaatgtgct cacaaaaaaa
     9661 aaccattagt ctgcttcctg ttcttacaat aatcagtatt atgtatttta tttatatagc
     9721 aaaatatgct tgttacacct gtcataatta ggcatggtaa aattattgac tgaaccctgg
     9781 gggagaaaga tgcacagtga ttattataat atggtttttg gtgaaaaatt ggccaatatt
     9841 ttatatgaag ctaactctca atttttttac gagagaaatg tcatagaaga ggcagtcaac
     9901 gctttattct gtgagcgtga aataataaac aacaagaaca taataaagaa gctgatgttc
     9961 tttttgtcag atgttaatca caccaagaaa gatgttgttc agagcgcact taatattatc
    10021 attgacatta catcaggtga tatttgagat tttacataaa agataatctc tcaaggagtc
    10081 ttatctgaaa acgaaatata tagtccctct cgttttgtaa tatcgattgg ctttgttgaa
    10141 tcggttacgt accccggagc cggtcatatt ttcaggtaat acacacgctt tcagacattt
    10201 cggctttcgt catttttgta tcatgggcat agcttctgct acctgaccat cataatcacg
    10261 cagcttcagt gaagcagtga ccacctgttt aacccggtgt atcgttatga tcttatccag
    10321 ca
//
LOCUS       DAJTOR010000144        10320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTOR010000144 DAJTOR010000000
VERSION     DAJTOR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.144"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            121..987
                     /gene="folD"
                     /locus_tag="OO249_003213"
     CDS             121..987
                     /gene="folD"
                     /locus_tag="OO249_003213"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5637407.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQACIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            989..1201
                     /gene="ybcJ"
                     /locus_tag="OO249_003214"
     CDS             989..1201
                     /gene="ybcJ"
                     /locus_tag="OO249_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5637408.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1309..1830
                     /locus_tag="OO249_003215"
     CDS             1309..1830
                     /locus_tag="OO249_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5637409.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMEMLWWRRR"
     gene            complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OO249_003216"
     CDS             complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OO249_003216"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5637410.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3425..3919
                     /gene="ppiB"
                     /locus_tag="OO249_003217"
     CDS             3425..3919
                     /gene="ppiB"
                     /locus_tag="OO249_003217"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5637411.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3922..4644
                     /gene="lpxH"
                     /locus_tag="OO249_003218"
     CDS             3922..4644
                     /gene="lpxH"
                     /locus_tag="OO249_003218"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5637412.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4762..5271
                     /gene="purE"
                     /locus_tag="OO249_003219"
     CDS             4762..5271
                     /gene="purE"
                     /locus_tag="OO249_003219"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5637413.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5268..6335
                     /gene="purK"
                     /locus_tag="OO249_003220"
     CDS             5268..6335
                     /gene="purK"
                     /locus_tag="OO249_003220"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5637414.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETVLTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YMGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6472..7363)
                     /locus_tag="OO249_003221"
                     /pseudo
     CDS             complement(6472..7363)
                     /locus_tag="OO249_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(7360..8175)
                     /locus_tag="OO249_003222"
     CDS             complement(7360..8175)
                     /locus_tag="OO249_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR5637415.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8186..9445)
                     /locus_tag="OO249_003223"
     CDS             complement(8186..9445)
                     /locus_tag="OO249_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR5637416.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGGCLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9455..10320)
                     /gene="fdrA"
                     /locus_tag="OO249_003224"
                     /pseudo
     CDS             complement(9455..10320)
                     /gene="fdrA"
                     /locus_tag="OO249_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2272 a         2930 c         2770 g         2348 t
ORIGIN      
        1 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
       61 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
      121 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
      181 caaaaagttc aggcgtgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
      241 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
      301 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg
      361 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
      421 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
      481 gacggtttcc atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
      541 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctt
      601 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg
      661 ctcgcaggtt gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
      721 gaaaatgccg atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
      781 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
      841 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccg
      901 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgcttca ggcgtgcgtt
      961 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     1021 cgttgagctg tgcgatttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     1081 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     1141 caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     1201 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     1261 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     1321 attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     1381 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     1441 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     1501 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     1561 gggcttattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     1621 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     1681 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     1741 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     1801 atggaaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     1861 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     1921 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     1981 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     2041 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     2101 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     2161 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     2221 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     2281 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgtct gcttcaggtt ctcttcgctg
     2341 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     2401 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     2461 cggtcaacca tcaccattcc ggagtgcatc cagtagttca catactgacc atcatgggca
     2521 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     2581 tggatatcaa agtggttacc cagctgcttg cagttcatgg ccgaacattc aatgtgccag
     2641 ccaggacgac ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     2701 cacagaacga agtccatcgg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc
     2761 tgcagctggt cgagatcctg acgcgacagc acgccataag tcggatcggt cgggacgtcg
     2821 aacatcacgt cgccgttgtc agccacataa gcgtgacctt tggcgatcag ttgttcagtg
     2881 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     2941 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     3001 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     3061 acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     3121 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     3181 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     3241 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     3301 catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     3361 aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg aacaggatgc
     3421 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgatgataa
     3481 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     3541 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     3601 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc tgaaaaatac
     3661 ccgtggtacg ctggcaatgg cccgtactca ggctccgcac tctgcaactg cacagttctt
     3721 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     3781 ctactgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt
     3841 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     3901 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     3961 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc tcgcaaggcc
     4021 gacgcgctgt atattcttgg cgatctgttt gaagcgtgga ttggcgacga cgatcccaat
     4081 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     4141 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagtggc
     4201 atgacgttat tgccggaaga aaaggtgctc gaactttatg gccgccgggt gttgattatg
     4261 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     4321 aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     4381 gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac
     4441 gttaaccaaa acgcagtggt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     4501 gggcataccc atcgcccggc ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc
     4561 gtggtactgg gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt
     4621 gagctgattc attttccgtt ttaaaaaaac cacaactttg ctgattttac agccacgcaa
     4681 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     4741 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     4801 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     4861 aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     4921 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca
     4981 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     5041 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     5101 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     5161 gcacaaattc tcgcgactca cgataaagag ctacaccagc gtctgaatga ctggcgcaaa
     5221 gcccagaccg acgaagtgct ggaaaatccg gacccgcgag gtgcggcatg aaacaggttt
     5281 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
     5341 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg
     5401 tgattaccgc tgagatcgaa cgctggccgg aaaccgtatt aacccgcgag ctggcgcgtc
     5461 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc
     5521 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
     5581 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
     5641 gctatgacgg tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag
     5701 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
     5761 ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
     5821 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc
     5881 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatatgggc gtgatggcga
     5941 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
     6001 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
     6061 cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc
     6121 tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt
     6181 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
     6241 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
     6301 gcggcgtgat ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat
     6361 gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg
     6421 cgtcaagcgt cgaatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga
     6481 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6541 cgcgggttta ctacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga
     6601 accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg
     6661 ctgcggcgtt ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc
     6721 ggcattaatc tgctcggcga gcaacgcagt ggcgagatct ttatcaatca ccgcttcact
     6781 ccctgcccca tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc
     6841 ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc
     6901 caccacccgg cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag
     6961 tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc
     7021 cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gtatctgcgg
     7081 ctgtgcgctc aagctctgcg ccagcatata gcctcatccc ctggctttcc gccaccagca
     7141 catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc agcagcccca
     7201 cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg cgtgccagcg
     7261 cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc tcaccgcgct
     7321 ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa
     7381 ttgttggcca agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag
     7441 cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgga caaagtgcag
     7501 gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt
     7561 ggtaacgagt tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa
     7621 agcgccataa taccaagccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt
     7681 taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc
     7741 ctgaaaacag tgacgaaact ggcgtaattc agcggtcagc ggatcgcttg ccgccatcgc
     7801 cagtggtccg aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg
     7861 aggatgcgaa ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa
     7921 acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc
     7981 gcataacgca tcgaattgcg cgcggcgaag cacccatcct ccggggccga aaccactacc
     8041 ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc aggttaatcg cccgccgcca
     8101 cactcccgct aaccgccagg actggcgata attcggtgcg ctactgctgg caagcagagg
     8161 atggatgatc gtcattgcgc gttcctcaac caatccccat gctttcagcc agtgcttcca
     8221 gcgcctgttc aaagcacgcc agcggtgccc gcacggtgcc tgcgccaatc tgcccgatcc
     8281 ccgcctcttt atgggcgata ccggtattga tgagtggcgt aatgccggtt tctaccacgc
     8341 gacgaatatc cagccccagg cacgcgccct gaaaatccca gcctgggatc tgcaactgca
     8401 tattgcgttc aaggtaaatt tccgccatct cttcagatac cgccctggcc gcttccatgc
     8461 cacccgcacc gacaaagcgc gttacgccag gcgctgcgat cattgccgca cctccgatac
     8521 caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc
     8581 cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt aaaccagcgt tcacccagcc
     8641 cgcttacccg aatcccgaac atattgccgt tgcgggtcat agccgtaacg atgctgcctg
     8701 cgcggatcat cgcgccagca tccatcgccg ccttgcagta ggccatcgcg aggttgagga
     8761 agaactgatc ggtcacgctg aggaaatcga tcacttccgc aatgtgctgt ttatcatgat
     8821 cgaggcgagc aatttgtggg gccagcgtac gcattaacag tgctgaggaa gcaatattgc
     8881 gttgatggaa ctcatcgccc atcgtaatgc cctgcgccat catcgccgtg agatcgatac
     8941 cgcgctccat gcgccccagc gccgcgctta atactggcat taacacatcg cgcatccagc
     9001 gatggcgagt caggacatct tcgccgtaag cgccaaaacg catcactttg ccgatacctt
     9061 cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc agcatcggca
     9121 tactggcaga agtaatgccg cccattggcc cgacggcatt cacatggtga caaggaatga
     9181 agttcacttc cccctgctcc agcatcgcca gtgcctgcgc ttcatctttc gcccagcctt
     9241 cgaacagaca tccgcccacg cacgcccctt tcatgggtcc ggtcatctcc tgccagcgca
     9301 ttggcggccc ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata agtgaaaaag
     9361 ccggttgcac atccagccag tgtggacgag cacgacgaat ttgttcgata accgcagcat
     9421 tggcttgcgc cactgatgta aacatgggaa ccccttattg caaacgttct aataaacgag
     9481 ccagtttttt attgccgccg gcgactggcg accattggta atgcacaacc ggtttgctgg
     9541 cgctttgtag ctccagcgca aagctgcgta atccgatgtt aatcacggcg acgttttcca
     9601 gtaatgacgg tgtatgttgc tgtgtggcag gcgagagcgg ataaattaac gctgccgcca
     9661 gcaaggtggc ttccggcagc gaactcacta ccgcgatccc cgcatcttcc agcgtggcga
     9721 tttgctgcga gcggcattgc gggtcacgtt cagtgcctgt caccgtggca atggcataca
     9781 gtggttgatt atctgaacgc gcggcacagg ctttttgcta ggcgcttacc agcgaggcgg
     9841 caggatcggc agtcgcaccg aagccaatca cgacatcaag tagcaatacg cgcacttgtg
     9901 gtttagcgcc gagatcggca attaactggt tgcgtaaggc tgggtcgatc atcggatggg
     9961 gacgcccgac ggtgtagaaa tcatcgccga ggtcaataat ctggtgacca tcggcgtcga
    10021 gcatcatgcc gtgatgatgg gtatcgtcgg cttccacgcc aaggtgtcca gcaagtaatc
    10081 ccgccgcttc ggcagccagc gtaccgccgg tatacaaacc gcaaataaat ccgctgctga
    10141 caggcgctat tgcgttacat cgcgccgtga cgcgtgaaag caggcaagcc aggcgtgcgg
    10201 cctcatccag cgaggaggca aaccagacat tctcgtcgcg ggccaccgcc ggggtataac
    10261 ctaaaaacag cgccaccatc ggtttgccgg ttgctttcat ggcattaaca attttcagac
//
LOCUS       DAJTOR010000145        10291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTOR010000145 DAJTOR010000000
VERSION     DAJTOR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.145"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            119..748
                     /locus_tag="OO249_003225"
                     /pseudo
     CDS             119..748
                     /locus_tag="OO249_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004129194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            745..1359
                     /locus_tag="OO249_003226"
     CDS             745..1359
                     /locus_tag="OO249_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR5637417.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1416..2873)
                     /gene="pepD"
                     /locus_tag="OO249_003227"
     CDS             complement(1416..2873)
                     /gene="pepD"
                     /locus_tag="OO249_003227"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5637418.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3134..3592
                     /gene="gpt"
                     /locus_tag="OO249_003228"
     CDS             3134..3592
                     /gene="gpt"
                     /locus_tag="OO249_003228"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5637419.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3684..4928
                     /gene="frsA"
                     /locus_tag="OO249_003229"
     CDS             3684..4928
                     /gene="frsA"
                     /locus_tag="OO249_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5637420.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4986..5387
                     /gene="crl"
                     /locus_tag="OO249_003230"
     CDS             4986..5387
                     /gene="crl"
                     /locus_tag="OO249_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5637421.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5426..6481)
                     /gene="phoE"
                     /locus_tag="OO249_003231"
     CDS             complement(5426..6481)
                     /gene="phoE"
                     /locus_tag="OO249_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5637422.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6769..7872
                     /gene="proB"
                     /locus_tag="OO249_003232"
     CDS             6769..7872
                     /gene="proB"
                     /locus_tag="OO249_003232"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5637423.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7884..9137
                     /gene="proA"
                     /locus_tag="OO249_003233"
     CDS             7884..9137
                     /gene="proA"
                     /locus_tag="OO249_003233"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5637424.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9252..9327
                     /locus_tag="OO249_003234"
     tRNA            9252..9327
                     /locus_tag="OO249_003234"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9285..9287,aa:Thr,seq:cgt)
     gene            complement(9342..>10291)
                     /locus_tag="OO249_003235"
     CDS             complement(9342..>10291)
                     /locus_tag="OO249_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5637425.1"
                     /translation="KKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHE
                     ENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIK
                     VPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRR
                     VAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRK
                     MRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRR
                     YAHLAPNHLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
BASE COUNT         2465 a         2540 c         2683 g         2603 t
ORIGIN      
        1 ctattagcgg ttaccagcgc cccaccagac ccggtgacat caccccccgg atcatggacg
       61 actgggggca gtaatcatgc cgggtctggc tggctaacac cccattataa ggggtacgaa
      121 taaagtaacg gggcagtcta ttttacagcg gcatattgcc tcgttctcgc atcatggttt
      181 gcctcaaggc agtgacgacg cgctacggta tattgcggaa catgacgatg cgctggcgtt
      241 tgcgtgtatt aatcgccaga tgatcgcttt gcgcataatg caacaagtta aggccacggg
      301 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcaaatcg gcgatcaaca
      361 gggctggttg catcgtaaag gtgccacgcc ggatgacaac ggtctggtga ttattcccgg
      421 ttcacgtggt gattactcct ggattgtaca gcctgttgcg aacgaaaaaa cgctccactc
      481 gctggcgcat ggggcagggc gtaaatgggg gcgcaccgag tgtaaatggc gtctggcagc
      541 gaaatacacg gtgacgcaac tctcgcggac tgaacttggc agtcggataa tttgtcgcga
      601 taaacaactc atctttgaag aagcgccaca agcctataaa tcggctgaaa gcgtggtgca
      661 atgtctggtg caggccggtt taattattcc tgtcgcgcgg ctgcgtccgg tgctaacgct
      721 caaaaacagt ggagggaaaa aaggatgatc ttgctacaac tctcctcagc gcaggggccg
      781 gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc taattaaaga agctgcccga
      841 caagacgtcg cggtaacggt gttggaaaca gaaacgggcc gctactctga cacgctgcgt
      901 tcagctctgg tttctctgga tggcgataac gcatgggcat taagcgaaag ctggtgtggc
      961 actattcagt ggatttgtcc gagtccgtat cggcctcatc atgggcgcaa aaactggttt
     1021 ctgggcattg ggcgttttac cgctgatgag caggagcaat ccgatgcgat ccggtatgag
     1081 acgctgcgtt cgtcggggcc gggcggtcaa catgtcaata aaaccgactc ggcggtacgc
     1141 gccacgcatt tggcatccgg tattagcgtg aaggttcagt cagagcgtag tcagcatgct
     1201 aacaagcggc tggcacgatt gctgattgcc tggaagctgg agcagcagca acaggaaaat
     1261 agcgcggtgc tgaaatcgca gcggcgaatg ttccatcacc agattgaacg tggcaacccg
     1321 agacggacat ttacagggat ggcttttatc gaaggataat gaaggatgaa actgccggaa
     1381 ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat ttctttcagc
     1441 agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg ctcatccgga
     1501 gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg atacggtttt
     1561 ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt cttgttgaac
     1621 aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc cggctgccag
     1681 ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag cgaatccagc
     1741 atgctcacca cgtagtcttt accgctgtcg atcagtgaac ggatcaggca gtgaatttct
     1801 acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcaac cacacctttg
     1861 gctacatcgg agttgcggat cacaccgttc ggggtggcgt tcagcagacg aataaaggta
     1921 tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag caacaaggcc
     1981 agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt attcaccaga
     2041 gatttcagtg cgtcgacttt atcagctgcg acagcaatgg tcgcaaaggc ttcacgcggg
     2101 atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag ttcttctgca
     2161 tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac gtggatttca
     2221 ccgccggagt gaccgccttt cagacctttt aaggttaact tgaaggtttc aaaaccagct
     2281 ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc cgcacaaccc
     2341 atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc ctgcaaccag
     2401 ttgctctgta agccaaaagc accgtccata ccggcttctt cggtcatggt cagcagcact
     2461 tccagcggac cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga ggccatacca
     2521 atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc atcaatataa
     2581 ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg cggcaccata
     2641 tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt agcaggttta
     2701 cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa
     2761 ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgcggaat agaacagatt
     2821 ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga cacgttaagt
     2881 ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag aaaatgttgt
     2941 caacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag
     3001 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3061 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3121 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3181 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3241 ccgtggcggt ctggtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3301 taccgtttgt atttccagct acgatcacga caaccagcga gagcttaaag tgctgaaacg
     3361 cgcagaaggc gatggcgaag gcttcatagt tattgatgac ctggtggata ccggtggtac
     3421 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3481 ggctggtcgt ccgctggttg atgactatgt tgttgatatt ccgcaagata cctggatcga
     3541 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     3601 acgcctggca ctgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     3661 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     3721 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     3781 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     3841 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     3901 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgatactgtg attggctacc
     3961 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4021 gtgcggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4081 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4141 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4201 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4261 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4321 tgtatgaacg ttattttgcg ccgcgcggca ttgtgatgct gactattgat atgccgtcgg
     4381 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4441 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4501 gtttcggcgc taacgttgcc gtgcgtctgg catatcttga atcgccgcgt ctgaaagcgg
     4561 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     4621 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     4681 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctgggacgtc
     4741 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     4801 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     4861 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     4921 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     4981 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5041 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5101 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5161 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5221 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5281 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5341 aaccggcgga tgattttcgt gacgagccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5401 gatgcatcac atccggcaat atctattaaa actgatacgt catcccaatc gcgacaatat
     5461 catcattatt aatattcaat ttgttatcgc tatccagttg gttgatttta taatcaacaa
     5521 atgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttaaccagat
     5581 cttcaccacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     5641 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     5701 agccgccagt tattggcgtc attttgcgag tttcagaata gaaagttgcc agataaatat
     5761 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     5821 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     5881 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     5941 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6001 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6061 cggtctgcgc cgaggagtcg ccaccaaatt ccggaaacat atcggtccag gcttccacgt
     6121 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6181 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctctgcttta ttaccggcaa
     6241 actctgcttc ccaacgacca taaccagtca gttgatcgtt aatttgtgtt tcgcctttaa
     6301 aaccaaaacg gatataactc tggtcgccat ctttactgtc gttatcactc atataatgca
     6361 tggctttaac tttgccatag acatccagtt tattaccgtc tttgttatat atttctgcgg
     6421 cctgtacgga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6481 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6541 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaataata ttcttaaaat
     6601 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctaggc ttgctgatcc
     6661 cgcgcaacaa aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta
     6721 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
     6781 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
     6841 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
     6901 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacttggg ttacccggaa
     6961 ctgccagcga ctatcgcctc gaaacaactg ctggcagcgg tagggcagag tcgactgatt
     7021 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc
     7081 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
     7141 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
     7201 aaagtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
     7261 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg acccgcgcag caatccgcag
     7321 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
     7381 agcgtttcag gcctcggaac tggcggcatg agtaccaaat tgcaggccgc tgacgtggct
     7441 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
     7501 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
     7561 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     7621 actgccgcca ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     7681 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     7741 cacggtgtca gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa
     7801 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     7861 attacccgtt aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct
     7921 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     7981 atgaactgga agcacaaagc gaaatcattc tcaacgctaa cgcccaggat gttgctgacg
     8041 cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     8101 tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg
     8161 taatcgatgg cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     8221 gagttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     8281 gcctgaaaac cggtaatgcg gtgatcctgc gcggtggcaa agaaacgtgt cgcactaacg
     8341 ctgcaacagt ggcggtgatt caggacgccc tgaaatcctg cggcttaccg gcgggtgccg
     8401 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
     8461 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
     8521 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     8581 tagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     8641 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     8701 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcactggcgc
     8761 agttgcaggc aggccctgcg aaggtggttg ctgttaaagc cgaagagtat gacgatgagt
     8821 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     8881 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ctgcgatatg cgcaacgccc
     8941 agcgttttgt taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta
     9001 ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg
     9061 cgcgtggccc aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt
     9121 acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc
     9181 cattgacgca tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat
     9241 gtcctttcag ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg
     9301 taggttcgac tcctattatc ggcaccattt aaatcaataa gttacacatc attagtacgt
     9361 tccttatttc ttgactggga caaatttggg accgatgggt tcagaatcga gtctatttgc
     9421 cgtgcgtgtt cggtaaggtg attaggtgcg aggtgagcat atcgacgaac catttcgata
     9481 gactcccagc ctcccatttc ctgtaacact gataacggga ctccggcttg aaccagccaa
     9541 cttgcccagg tgtgtctcaa gtcgtgaaat ctgaaatcat caatacctgc tcgtctcagc
     9601 gccgctttcc aggctgtgtt tgcgtcatac cgcatcttcc tgactgttgg cgctttcgtt
     9661 ccgtctggtt tggtacagct ttccttatac acaaataccc aacggtgatg attcccgatt
     9721 tgttttttca atacgcgaca tgcagtatca ttcagcgcaa cgccaattgc gcggtttgat
     9781 ttactctctt ccgggtttat ccatgccacc cggcgctgca tgtctatttg ttgccattca
     9841 aggttgatga tgttcgagcg tcttaagcct gttgcaagtg caaattcaac aacagacttt
     9901 aatggctccg gacattcatc aatcagcctt tgtgcttcat gaggctccag ccagcggatc
     9961 cgtttattct ttggttgagg cactttaata attggtgcct tatccagcat tttccattca
    10021 cgctctgcgg ctcttagcag ggcctttata aatgaaagat gcgtagcctt cgttgcaacg
    10081 gacgctggtt ttggcgtgta ttctggaaca ggtttccctt tttttctgca tgcttctgcc
    10141 ctgagtttcc agttttcctc atgacgccgg ttcgtcattt tctgcattgc tgaataaatt
    10201 tttgattcag taatgtctct tagttgcatt cctgcgaaat gttgaagcca gaatccgatc
    10261 cggcttttgt catcgtccag tgatttttta t
//
LOCUS       DAJTOR010000146        10285 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTOR010000146 DAJTOR010000000
VERSION     DAJTOR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.146"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            669..1034
                     /locus_tag="OO249_003236"
     CDS             669..1034
                     /locus_tag="OO249_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5637426.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1074..1778
                     /locus_tag="OO249_003237"
     CDS             1074..1778
                     /locus_tag="OO249_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR5637427.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGE
                     NEGVDHKVIDAFSPAG"
     gene            1845..3263
                     /gene="dcm"
                     /locus_tag="OO249_003238"
     CDS             1845..3263
                     /gene="dcm"
                     /locus_tag="OO249_003238"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5637428.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3244..3714
                     /gene="vsr"
                     /locus_tag="OO249_003239"
     CDS             3244..3714
                     /gene="vsr"
                     /locus_tag="OO249_003239"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5637429.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3703..4623)
                     /gene="yedA"
                     /locus_tag="OO249_003240"
     CDS             complement(3703..4623)
                     /gene="yedA"
                     /locus_tag="OO249_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5637430.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4796..5713
                     /locus_tag="OO249_003241"
     CDS             4796..5713
                     /locus_tag="OO249_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5637431.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5792..5974
                     /locus_tag="OO249_003242"
     CDS             5792..5974
                     /locus_tag="OO249_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5637432.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6145..7839
                     /gene="yedQ"
                     /locus_tag="OO249_003243"
                     /pseudo
     CDS             6145..7839
                     /gene="yedQ"
                     /locus_tag="OO249_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(7836..8651)
                     /locus_tag="OO249_003244"
                     /pseudo
     CDS             complement(7836..8651)
                     /locus_tag="OO249_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(8949..9165)
                     /gene="yodD"
                     /locus_tag="OO249_003245"
                     /pseudo
     CDS             complement(8949..9165)
                     /gene="yodD"
                     /locus_tag="OO249_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            9328..9516
                     /gene="dsrB"
                     /locus_tag="OO249_003246"
     CDS             9328..9516
                     /gene="dsrB"
                     /locus_tag="OO249_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5637433.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9560..10183)
                     /gene="rcsA"
                     /locus_tag="OO249_003247"
     CDS             complement(9560..10183)
                     /gene="rcsA"
                     /locus_tag="OO249_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5637434.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
BASE COUNT         2626 a         2428 c         2670 g         2561 t
ORIGIN      
        1 gccgtagaaa tcaactttat tgccatcttt attgtaaatt tcagccgcat ttgctgcgcc
       61 agcaactaat aacgccggga ccagcattgc cagaactttt cttttcatta tgtattccct
      121 tgtgattata atcttcatga atatatcaat aagtgccgtt atccaaaaaa aagcacattt
      181 ggatactatt ctatgaagtt catttcattt taagtattac atgcaacaaa tgtatttaaa
      241 agatttcaaa atgtttctaa tctatttaat ataattcaga gatatgaatt ataaatattc
      301 atgaaaatta taaatgtata tcaatatgtt atgagttgag cggaaacata gggggtatca
      361 atgattgttg cgccaaacgg ctggtgtgat caggcgcaca ttaatgaagg cataaaaaaa
      421 agccggcatc atgccggccc aaaaaatata aacaagacga taaaaggcta ataacggaag
      481 catcatgaca cagaataata aaaaggtata attgtaatca gtgctaatat attgattaaa
      541 tgaattaata aagccgatat caaagataaa taaaatcgct caaaacgatt tttcctgtat
      601 tcactgccgt tgcgcaaaat ttatctattt gttcaaaaaa tgattgagtc ttgactggct
      661 catccaatgt ggaaaaatgt gacttttatc acataatagt gctaagtctg aattttccgg
      721 gttatctcaa aatggaatac ggttcgacaa agatggaaga gagactctct cgcagccctg
      781 gcggcaaact ggctttgtgg gctttctata catggtgtgg ctatttcgtc tgggcaatgg
      841 cgcgttacat ctgggtgatg agccggatcc ccgatgcccc cgtgtcggga tttgaaagcg
      901 atttaggctc taccgcggga aagtggttag gggcactggt cggattttta tttatggctc
      961 tggtcggggc attgttaggc agcattgcct ggtacacccg acctcgtccc gcacgtagca
     1021 gacgctatga ataaaaactg ctaaggtggc gactctttgg caacggagcg cgtatggact
     1081 tacaacactg gcaggcacag ttcgaaaact ggttgaaaaa ccatcatcag caccaggacg
     1141 cggcccatga cgtgtgtcat tttcgccgcg tctgggctac ggcacagaag ctggcggcag
     1201 atgacgatgt cgatatgctg gtgattttaa ccgcctgtta ttttcacgat attgtgagcc
     1261 tggcgaaaaa tcatccgcaa aggcagcgtt cttcaatcct ggcggcagaa gagacgcgtc
     1321 gcctgctccg tgaagagttc gagcaatttc cggcggagaa aatcgaggcc gtttgtcatg
     1381 tcattgccgc tcacagtttc agcgcgcaaa tcgccccctt aacaacggaa gctaaaatcg
     1441 tccaggatgc ggatcggctg gaagccctgg gggcgattgg cctggcgcgc gtgtttgccg
     1501 tttcaggggc attgggcgtg gcgctgtttg atggtgaaga tccgttcgca cagcatcgcc
     1561 cgcttgatga taaacgctac gcgctggatc atttccagac taagttgctg aagctaccgc
     1621 aaaccatgca aaccatgcaa accgcaagag gcaagcagct ggcgcagcac aacgcgcatt
     1681 ttttagtcga gtttatggcg aagctcagtg ccgaactggc gggggagaat gaaggtgtcg
     1741 atcacaaggt gatagatgcg ttttcacccg cgggataagc gcgtggccct aaatggctgt
     1801 aattatgtta acctgtcggc catctcagat ggccggttaa atctatgcag gaaaatatat
     1861 cagtaaccga ttcatacagc accgggaatg ccgcacaggc aatgctggag aaactgctgc
     1921 aaatttatga tgttaaaacg ctggtggcgc agcttaatgg tgtgggtgag aatcactgga
     1981 gcgcggcaat tttaaaacgt gcgctggcga atgactcggc atggcaccgt ttaagtgaga
     2041 aagagttcgc ccatctgcaa acgttgttac ccaaaccacc ggcacatcat ccgcattatg
     2101 cgtttcgctt tatcgatcta tttgccggaa ttggcggcat ccgtcgcggt tttgaatcga
     2161 ttggcggaca atgcgtgttt accagcgaat ggaacaaaca tgcggtacgc acttataaag
     2221 ccaaccatta ttgcgatccg gcgacgcatc attttaatga agatatccgc gatatcaccc
     2281 tcagccataa agaaggcgtg agtgatgagg cggcggcgga acatattcgt caacacattc
     2341 ctgaacacga tgttttactg gccggtttcc cttgtcagcc attttcgctg gctggcgtat
     2401 cgaaaaagaa ctcgctcggg cgggcgcacg gttttgcctg cgatacccag ggcacgctgt
     2461 tttttgatgt ggtacgcatt atcgacgcgc gtcgtccggc gatgtttgtg ctcgaaaacg
     2521 tcaaaaacct gaaaagtcac gaccagggta aaacgttccg catcatcatg cagacgctgg
     2581 acgaactggg ctatgacgtg gctgatgcag aagataacgg gccggacgat ccgaaaatca
     2641 tcgatggtaa acattttctg ccgcagcacc gtgaacgcat cgtgctggtg ggttttcgtc
     2701 gcgatcttaa tctgaaagcc gattttactc tgcgtgatat cagcgaatgt ttccctgcac
     2761 agcgagtgac gctggcgcag ctgttggacc cgatggtcga ggcgaaatat atcctgacgc
     2821 cggtgctgtg gaagtacctc tatcgatatg cgaaaaaaca tcaggcgcgc ggtaacggct
     2881 tcggttatgg aatggtttat ccgaacaatc cgcaaagcgt cacgcgtacg ctgtctgcgc
     2941 gttattacaa agatggcgcg gaaattttaa tcgatcgcgg ctgggatatg gccaaaggtg
     3001 agaaagactt tgacgatccg ctgaatcagc aacatcgtcc acgtcggtta acgcctcggg
     3061 aatgcgcgcg cttaatgggt tttgaagcgc cgggagaagc gaaattccgc attccggttt
     3121 cggacactca ggcctatcgc cagttcggta actcggtggt cgtgccggtc tttgccgcgg
     3181 tggcaaaact gcttgagcca aaaatcaaac aggcggtggc gttgcgtcag caagaggcac
     3241 aacatggccg acgttcacga taaggccact cgcagcaaaa atatgcgcgc gattgccacg
     3301 cgtgatacgg cgattgagaa gcgcctcgcc agtctgttaa ccgggcaggg cctggcgttt
     3361 cgcgttcagg acgccagtct gcccggaagc ccggattttg tcgttgatga atatcgctgc
     3421 gtgatattta cccatggctg cttctggcat catcatcact gctatctgtt taaagtgcct
     3481 gcgactcgaa ccgagttctg gctggagaag ataggtaaaa atgttgaacg cgatcgccgc
     3541 gatatcagtc gtttgcagga actcggctgg cgcgtactga ttgtctggga gtgtgcatta
     3601 cgtgggcgcg agaagctgac ggatgaagcg cttaccgagc gtctggaaga gtggatctgc
     3661 ggcgaaggtg ccagcgcgca gatcgacacg caggggattc atttactcgc ttgatgcgtc
     3721 ctgaataact ggcgcaacta cgggttttgc cgggaagaga tattttccca acgtgaccag
     3781 taccaccgcg aagacaatta cgccgagcgc cagccattca atcttcgaca gcgtctctcc
     3841 gcccagtccc gtacccagca agaccgcgac caccgggtta acgtaggcgt agctggtggc
     3901 gagagccgga ctgacattac ggattaaata catataagcg ttgatggcga taatcgaacc
     3961 aaacagcgcc agatagccga ccgcaaggaa gcctgaaagg gaagggagcg ccgtcagttt
     4021 ttcacctgca atcatcgacg cgatcattaa caccacgcct gccgccagca tctcaatcgc
     4081 acccgccatc atccctacag gtaaggtaat gcgcgagcca taaaccgagc caaacgccca
     4141 gctaatcgag ccgattaaaa tcagaatcgc gccccacgga ttgccgctta aatttccacc
     4201 gctattgagc atgatgattc cggcaagccc aatggcgata cccacccatt ccaatttgcg
     4261 cgttttaatg ccaaacaggc ggctgaagca cagggtaaag aggggcacgg ttgcaaccac
     4321 aacggcggcg atgccggaag gaacattttg atgctcggca accgtcacca tgccattacc
     4381 gacagccagc aataacaggc caatcagcgc ggcattgagc agcggacgta gcggggggag
     4441 cttgtgtccg cgcagtagca aaaatgccat caataaaata ccggctgcca ggaatcgaac
     4501 gcctgccatc attaacggag gccagctttc cacgccaatc cgaatgacaa aataggttga
     4561 gccccaaatg atatacaacg caaacagcgc gccaaaaagc ggtaacaact ggcggaaacg
     4621 cataatccct cacggtggaa ataaaaaggt ggttcatagt aaacgtgaaa atcattctgc
     4681 tggcgagaga tataattgca cttgattgtt aaaaaaatgt tgacctgtga agcaagtatc
     4741 agagctgccg tttttgcttc atacttacac ccttcaacaa taaaaatgag agggaatgct
     4801 tttggccggg agtagtttac tgacgttgct cgatgatatc gccacactac tggacgatat
     4861 ctccgtgatg ggcaaactgg cggcgaagaa aaccgccggt gtattagggg atgacttatc
     4921 gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg gaacttcccg tggtctgggg
     4981 cgtggcgaaa ggatcgctga ttaataaagt gattctggtg ccgctggcgc tgatcatcag
     5041 tgcgtttatc ccgtgggcga ttacgcctct gttgatgatt ggtggcgcgt ttctctgctt
     5101 tgaaggagta gagaaagtgc tgcatatgct ggaggcgcgt aaacataaag aagatccggc
     5161 gcagagccag cagcgtctgg agaagctggc ggcgcaggat ccgctgaagt ttgaaaagga
     5221 caaaataaaa ggggcgattc gtaccgattt tatattgtct gccgaaatcg tcgccatcac
     5281 gctggggatt gtggccgaag cgccgttgct taatcaggtg ctggtgcttt caggcatcgc
     5341 gctggtagtg accgtgggcg tctacggtct ggtaggggtt atcgttaaga ttgatgacct
     5401 ggggtattgg ctggcggaaa aatccagcgc gctgatgcag gcattaggta aagggttatt
     5461 gatcattgcg ccctggctga tgaaagcgtt atcgattgtc ggcacgctgg cgatgttcct
     5521 cgtcggcggc gggattgtgg tacatggtat tgcgccgctg catcacgcca ttgaacattt
     5581 cgccgggcag caaagtgcag tggtggcgat gatattaccg actgttttaa atctgattct
     5641 tggatttatc atcggcggca tcgtggtgct gggagtgaaa gccgtagcga aaatacgcgg
     5701 tcaggcacat taatactcgc acaggctatt atgcaaaatt cgccatcttc aactaaggtg
     5761 aacaagtttc actcgaaaaa aggaggcgag tatgagcttt atggttagtg aggaagttac
     5821 ggttaaagag ggcggcccgc ggatgattgt caccggatac tccagcggta tggttgagtg
     5881 tcgttggtat gacggttacg gggtcaagcg ggaagctttt catgaaaccg agcttgttcc
     5941 gggggagggg agtcgttctg cggaagaagt ttgaaagggt atcattaaca ccaggccgga
     6001 tcacttccgg cctggtgtgc caatgcctgc aatgaatgat agtcgcgtct tatcatgcca
     6061 ccattataaa attatcgctg ttttgcggta cgctatcgcc aggttatgcc agaatcataa
     6121 aaaagcaggt tgggagtcgt cagggtgcag cacgagacaa aaatggaaaa ccagagctgg
     6181 ttgaaaaaaa tcgcacgccg cctggggcct ggtcatgtcg ttaatctctg ctttatcgtg
     6241 gtattgcttt tttccacctt gctgacctgg cgtgaagtgg tagtgctgga agatgcctat
     6301 atctccagtc agcgtaatca tctggaaaac gtagccaacg cgctcgataa gcatttgcag
     6361 tataacgtcg acaaactgat ctttttgcgt aatggcatgc gcgaagctct cgtagcgcca
     6421 ctggatttca cttcactgcg taatgctgta accgagttcg aacagcatcg cgacgagcac
     6481 gcctggcaaa ttgaactcaa ccgacgacgc accctgtcag tcaatggcgt atcggatgca
     6541 ttagtcagcg aggggaatct cctgtctcgc gaaaatgaaa gcctcgacaa tgaaattacc
     6601 gctgcactgg aagttggtta cttgctgcga ctggcgcaca acacctcgtc gatggttgaa
     6661 caggcgatgt atgtctcgcg tgccggattt tacgtttcga cgcagccgac cttgtttacg
     6721 cgcaatgtac caacgcgtta ttacggctat gtcacccaac cctggtttat cggccattcg
     6781 caatgagaaa atcgtcaccg cgcggtacgc tggtttactt cgcaaccgga acacgccagc
     6841 aatactgaac cgcaggttac cgtcagtgtt ccggtagaca gtaataacta ctggtatggc
     6901 gtgctgggga tgagtattcc cgtgcgtacc atgcagcaat ttttaagaaa cgccatcgat
     6961 aaaaacctcg atggtgagta tcagctctat gacagtaagc tgagattttt gacctcttcc
     7021 aatcctgacc atccaacagg gaatattttt gatcctcgtg aactggcctt gctggcgcag
     7081 gcgatggaac atgacacgcg gggcggcatt cgtatggaca gtcgctatgt tagctgggaa
     7141 cgtctggacc atttcgacgg tgtgctggtg cgcgtccata cgctaagcga aggcgtgcgc
     7201 ggcgatttcg gcagtatcag cattgcatta accctgctgt gggcgctctt taccaccatg
     7261 ttactcatct cctggtatgt gattcgccgg atggttagca acatgtatgt tctgcaaagc
     7321 tcgttgcagt ggcaggcgtg gcacgacacc ttaacccgtt tatataatcg tggcgcactg
     7381 tttgaaaaag ctcgtccgct cgcgaaattg tgtcagacgc accaacatcc tttttctgtc
     7441 attcaggtcg accttgacca ttttaaagcg attaatgacc gctttagtca tcaggcgggc
     7501 gaccgtgttc tttctcatgc tgccggatta attagcagtt ccttgcgtgc gcaggacgtt
     7561 gccgggcggg tcggtggtga ggagttttgt gtgattctgc caggcgcgag tctgacggag
     7621 gctgcggaag tcgcagaacg tattcgcctg aagttaaatg aaaaagagat gttgattgct
     7681 aagagtacga cgatacgcat cagtgcctcg ttgggggtaa gtagcagcga ggaagccggt
     7741 gattatgatt ttgaacaact ccagtcactg gctgaccgtc ggctttatct cgctaaacag
     7801 gccgggcgta atcgggtatg cgcgagcgat aacgcttaac gagcggagaa aaaatggtcc
     7861 agcccttcac gccatccttc cggtccttca cgctgcgttc gccagacgcg ggccggatcc
     7921 tcatcatgca gatgcacccc ttcacggttt agccctttca caatcaccgc gtaatccatt
     7981 acctccagta agggcgcatc gtttggccca tcgcccaggc caagtgtggt tgggcgtttg
     8041 cctgataatt gttgataggt cgcgataatc cagttggcgg cctgatcttt tccggcagag
     8101 gcatccagga cgtgccagaa gcgcgcaccc tgcataaact gtaagcccag ttcgttcaga
     8161 cgagcggtaa attgtgccat acgctcgtca ctgtcgcgcc agattagcgt taccgacgcc
     8221 tcatgcagct gcgtcagcgc cgcctggcta cggcttaatc ccgtccattc ggcgatggtt
     8281 gcatcgtcga catcatcgaa agtcgtgaat ttaaaatgtt ctttctcgcg tagcgtattt
     8341 aaaacctggc tgatttcgcc atggctaata cctgagatga tgcgtggaaa accgtctata
     8401 tcctgccatt gttcagcaag ctggatcact gcgccatttt cggcaatcag tggtacccct
     8461 tgtaacccca acgttttttg caagtacagc atttccgctg aagttttact gctacagaga
     8521 atgacgggaa cattcgcttc gcgtaaacgg ctgagctagg gggcagccgg ttgccagtca
     8581 taactatgac tgtccagcag ggtgccatca agatcgctaa aaaccagtag tggttgttga
     8641 attgaaaaca tggtcgcgca gttctcccct taccaggatg attctcaaaa cagcacaaat
     8701 aatcaaagat gattttttcg ggagcgatcc cgttgacgcc agagccacca aggggtgacg
     8761 cacgtcacat ttctattcat aagtagcgtt aatcattcat atcgcgaata ttttcttgtc
     8821 agcgaaaaaa attgcggata aggtgatgaa cacatcagat ttcctggtgt aacgaatttt
     8881 ttaagtgctt cttgcttaag caagtttcat cccgaccccc tcagggtcgg gattttttta
     8941 ttgtgcattc aacgattcac ttcagagacg ctgaagtcat gaatatccag ttcgaaggca
     9001 tccgccaatt cacgccatgt atgatattca cgtccatcga cgctaacgtg ttcagaatcg
     9061 ttctggctgc gatgaactta tttcattgat cgccgccagc agggcatcga catcgacgct
     9121 gacctcacgt tttgcggtat cgctgtactc ttttgcggtt ttcatcatct ttcctcgcaa
     9181 ccgttactct gttgttgccg ccagtcgtta agtccgcctg gcatctctta agtatagacc
     9241 gtcgagaaaa tcagcactcg cgcgcgcctg gcgctgccag aaaaacgaaa ttgttctaca
     9301 ctggcacaaa gccacaggag gaaaacgatg aaggtgaatg atcgggtaac agtcaaaacg
     9361 gatggcggtc cgcgtcgtcc tggcgtggta ctagcagttg aggagtttag tgaaggcaca
     9421 atgtacctgg tttcgctgga agactacccg ctcggcatct ggttctttaa tgaagcaggg
     9481 catcaggacg gtatctttgt ggagaaagca gagtaatctt attcagcctg actggtggga
     9541 aaccaccagt cagaatgtgt tagcgcatgt tgacaaaaat accattagtc acattatccg
     9601 tcagtcggac gacatggtag ataacctgtt tattatgcgt tttgatctta cgtttaatat
     9661 tacctttatg cgatgaaacg gtcttggctt tgatattcat ttggtcagag atttgaatgg
     9721 ttccctgacc tgccatccac attcgcaaca tactcgattc ggttcggctc aatgataacg
     9781 tcggcatatt taaaaaagag gttatcgttg tctctttttt cagaatatcg ccaaggatat
     9841 cgtcgagaga ttccggttta atcgatttag aacttatcaa taaatttttt ctgaccaata
     9901 gatattcatc aaaatgaaca ttggcaattg ccataaaaac gataaataac gtattgggat
     9961 gttgattaat gatgtgcttg atgtgctgac tgttagaagc atcatggatg aaacagtcct
    10021 cattaataaa caccactgaa gggcgctgtg aatcacaagc tatggcaagg tcatcaacgg
    10081 tttcaatgtc gttgatttct ctttttttaa cccctctact caacagatac ccggttaaac
    10141 ctagtcgggt gtaactacat aaatccataa taatcgttga catggcatac cctcactcaa
    10201 tgcgtaacga taattcccct tacctgaata tttcatcatg actaaacgga acaacatggg
    10261 tcacctaatg cgccactctc gcgat
//
LOCUS       DAJTOR010000147        10243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTOR010000147 DAJTOR010000000
VERSION     DAJTOR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.147"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            18..1337
                     /gene="potE"
                     /locus_tag="OO249_003248"
     CDS             18..1337
                     /gene="potE"
                     /locus_tag="OO249_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5637435.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPCFELKNKHG"
     gene            1413..1562
                     /locus_tag="OO249_003249"
                     /pseudo
     CDS             1413..1562
                     /locus_tag="OO249_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            1686..2336
                     /locus_tag="OO249_003250"
     CDS             1686..2336
                     /locus_tag="OO249_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HCR5637436.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(2377..2871)
                     /locus_tag="OO249_003251"
     CDS             complement(2377..2871)
                     /locus_tag="OO249_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637437.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     RLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(3086..4726)
                     /gene="pgm"
                     /locus_tag="OO249_003252"
     CDS             complement(3086..4726)
                     /gene="pgm"
                     /locus_tag="OO249_003252"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5637438.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMEAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(4752..5297)
                     /gene="seqA"
                     /locus_tag="OO249_003253"
     CDS             complement(4752..5297)
                     /gene="seqA"
                     /locus_tag="OO249_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5637439.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5482..6246
                     /gene="ybfF"
                     /locus_tag="OO249_003254"
     CDS             5482..6246
                     /gene="ybfF"
                     /locus_tag="OO249_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5637440.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6386..6679
                     /gene="ybfE"
                     /locus_tag="OO249_003255"
     CDS             6386..6679
                     /gene="ybfE"
                     /locus_tag="OO249_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5637441.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            6819..7349
                     /gene="fldA"
                     /locus_tag="OO249_003256"
     CDS             6819..7349
                     /gene="fldA"
                     /locus_tag="OO249_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5637442.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7559..7645
                     /gene="uof"
                     /locus_tag="OO249_003257"
     CDS             7559..7645
                     /gene="uof"
                     /locus_tag="OO249_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5637443.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7638..8084
                     /gene="fur"
                     /locus_tag="OO249_003258"
     CDS             7638..8084
                     /gene="fur"
                     /locus_tag="OO249_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5637444.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8169..8495)
                     /locus_tag="OO249_003259"
     CDS             complement(8169..8495)
                     /locus_tag="OO249_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637445.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFPDQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8545..9951)
                     /gene="chiP"
                     /locus_tag="OO249_003260"
     CDS             complement(8545..9951)
                     /gene="chiP"
                     /locus_tag="OO249_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5637446.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPAYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2519 a         2496 c         2543 g         2685 t
ORIGIN      
        1 ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc gttcagttaa
       61 ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg
      121 ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc tcaatggcac
      181 tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggcggt atgggcggtt
      241 atgcggagta tgctttcggt aagtccggca attttatggc gaactacacc tacggtgtct
      301 cgctgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc
      361 tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggagtg ctgtggattt
      421 gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc attactgtgt
      481 ggggagtcat tattccggtc gtcggtctgt gcattattgg ctggttctgg tttagtccga
      541 cgctgtacgc taactcatgg aacccgcacc atgtgccgtt ctttagcgct gtaggttctt
      601 ccatcgccat gacgttgtgg gctttcctcg gtctggagtc agcctgtgcc aacgccgaag
      661 tagtggaaaa cccggaacgt aacgtgccga ttgcagttct tggcggtact ttaggtgcgg
      721 cggtgattta tatcgtctcc accaacgtga ttgccgggat tgtgcctaat atggagctgg
      781 caaattcaac ggcaccattt ggcctggctt tcgcacagat gttcacacca gaagtgggta
      841 aagtcattat ggcgctgatg gtgatgtcct gctgcggttc actgcttggc tggcagttca
      901 ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa attttctccc
      961 gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt attcagagtg
     1021 gattggcact gatgaccatt agcccgtcac tgaacagcca gttcaacgtg ctggttaacc
     1081 tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta gtcattattc
     1141 agaaggtcgc taatgtgccc ccctcaaaag cgaaagtagc aaactttgtt gcctttgttg
     1201 gtgcgatgta cagtttctat gcgctgtact catccgggga agaagccatg ctgtacggtt
     1261 ctatcgtgac cttccttggt tggacactgt atggtctggt atcaccatgc tttgaactga
     1321 aaaataaaca cggttaataa aacaaagtaa aaaagggcga ttgaatgatc gccctttctg
     1381 tttgttggca aatagttcgt tatttatgcc gcatccggca gatgtagggt ctaaacgcga
     1441 tcgaacacag caactatttg cgtaattata tggggcatct gcgacaaaaa ctggaacagg
     1501 atccggcccg gcctcgccat tttattactg aaaccggtat tgggtatcgg tttatgcttt
     1561 gaaaaaagtg gcaatgtatg gataaagtca ttttttattc aaatataagg aacttaatat
     1621 ttaaaaaatg ttccatacaa ttcctcatca taaatgacac cgtagcacac aaggagcaag
     1681 tagcgatgcg tgaaatgaat cagttagtaa atactgaaga ttcagcctgg ccaataatcc
     1741 agaactggtt aaaagacgcc acaaaccata ccgaactctt accggttaat aaaaatttag
     1801 ccgaaaccgc actttatcaa ttacaggtga caacaaaatc accgatgggt gcgcttgtct
     1861 atggctccgg aggtctgctg attgataatg gttggttacg catagcagga tctggtcacc
     1921 cccggttgcc acgagatcca gtaagttgga cacaacgccc tgaattcgca ggcgtgcggg
     1981 ctttacccat cgctgacgat gtcgcagggg gaatttttgc tcttaatggt ggcgatcttg
     2041 gggaagatac tggctgcgtt tattattttg cgccagatac gctgaattgg gaaagtctgg
     2101 aagttggata ttctgagttt cttcagtggg cattatccgg tgatttagat accttctatg
     2161 aaaatgtacg atggcagcaa tggcgagaag acgtaataaa attatcggca acggaagcct
     2221 tcacgttcta ccctttctta tgggtacaaa gtgaagaagc aagaacaaga aaagttattt
     2281 cgcttactga actctgggaa atgcagtatc aaatgaaaga aacattcacg caataagaca
     2341 tgttcaggtc cgggtttcat tcccggactc actattttaa tgtgtattta tcgctttcaa
     2401 ttcttgctgg caattttctg actggcagcg tcgtaactca ataatcccga catgcatcat
     2461 cgagccaaaa aatccgcttt cgctgacata atcctcattg actatcggtc gagtgccggg
     2521 cttgccctgg caaacccgca taaaaaatga ggtgtagcat tgccaaccgg gatttaaacc
     2581 gaccttttct tgtgctgaca tattatgaag ataagtaagt tctttttcgt tcccgcctga
     2641 caactgccag cggcgggtat tatcaggtaa gttatcacca cggcaatttg tcttacaccg
     2701 ggaaggtgca atatttaccg caatgctacc cgttaatctt ctggttttat ctttaggggt
     2761 cagtaaccag tcgccagcta atgatgattg ttcaggttgg gcgcatgcag tcagcagtaa
     2821 tgtggcggta attagtgaca ctatgacgac cagactcctg ttggtcttca ttgggatttc
     2881 cttgagctac tcagtttgtt gacaaagtgt tcgttgtttt tgccggatgc ggcgtgaacg
     2941 ccttatccgg cctacaaaat ctcgtaaatt caatatattg caccatcctc gtaggcctga
     3001 taagcgtagc gcatcaggca attctgtgtt tgtcatacgt aaaaaagggc gatcttccga
     3061 ccgccctttt tcttatttaa tgtgtttacg cgtttttcag aacttcgcta acaatctcaa
     3121 ccgcttcttt ctcaatctgc ttgcgatgtt cttcaccgag gaagctttcg cagtagatct
     3181 tatatgcgtc ttccgtgcct gacggacgcg cggcgaacca gccgttgtca gtcatcactt
     3241 tcagaccgcc gatagaagca ccattgcccg gtgccgccgt caggcgcgcg gtgatcgggt
     3301 cgcctgccag ggtgctggcg ctcaccattt ccggagacag cttagacagc gccgcttttt
     3361 gtgcggaagt cgcagctgcc tgcaaacggt tgtagctcgg cgcaccaaag cgttttgcca
     3421 gttcgttgta gtgttcctgc gggttcttac cggtgacagc ggtgatttcc gccgccagca
     3481 gacacatgat gatgccgtct ttgtcggtgg accacggcgt gccgtcgaaa cgcaggaagg
     3541 aagcccctgc gctctcttcg ccgccaaagc cgaagctgcc gtcgaacaga ccatcaacaa
     3601 accatttgaa acctaccggg acttctacca gcttgcggcc caagtcattg accacacggt
     3661 cgatcatcgc agaagaaacc agcgttttac cgacagcaac atctttgccc cactgcggac
     3721 gatgctggaa caggtagttg atcgccaccg ccaggtagtg gttcggattc atcaaacctg
     3781 ccggagtgac gataccgtga cggtcataat ccgggtcgtt agcaaacgcc agatcgaact
     3841 tatcacgcag tgccagcagg cccgccatcg cacactcgga ggagcagtcc atacggatcg
     3901 cgccgtcttt atcaaggtgc ataaagcgga aggtttgatc gacctgatcg ttaacgatgg
     3961 tcaggttgag gttgtaatac tcgccaatac gcttccagta ttcgataccg gaaccgccca
     4021 gcggatcaac gcccagcgtc aggcccgctt tctgaatcgc ctccatatca acgatatcgg
     4081 ccagaccttc cacaaacggc tgcaccagat cctgctcttt cacatgaccg gatgccatcg
     4141 cttcgtcgag ggagatacgc ttcacgcctt tcaggccatc ggccagcagt gcgttggccc
     4201 tgtcttccac cactttggtg acgttggtat cagccgggcc accatttggc ggattgtact
     4261 tgataccacc atcttccggc gggttatggg acggtgtaat cacgataccg tctgccagcg
     4321 gaccaccttt tttattgtga accaggatgg cattggaaac ggcaggcgtt ggagtgaagc
     4381 cattgttttc ctgcacaatg acatcaacgc cgttcgctgc cagcacttcc agtacggaaa
     4441 tgaacgcagg ctcggacagg gcgtgagtat ctttacccac atagcaaggg ccagtgatgc
     4501 cgtttttcgc acgttcttca gcaattgcct gagcgatcgc cagaatgtgc ggctcgttaa
     4561 agctgtggcg cgctgcactg ccacggtgac cggaagtacc gaatttcacc gcgtgctccg
     4621 cattccctgc ttctggtttc agtacataat attgcgccgt cagttgggcg acgttaatca
     4681 aatcactctg ttgtgcaggt tggcctgcac gattgtggat tgccattgct ttgtcctttg
     4741 tctgcaacgt tttagatagt tccgcaaacc ttctcaatca attccgccgg gaattgcatc
     4801 gactgcatga tgtgttcgat catgctgcat ttacggccgg tgttggtgtt ggtgatcacc
     4861 caatacggcg tgcctggcac atgtttcggc ttggtctgat taccattttt cagcagcgtt
     4921 tgttcatctg ccgcaaagta aacgcgtgta cgaccgtgca acgattccgt tgcttcggca
     4981 aacgcctggg cgtcaagaga atatagtgta gacaacagca gcataaagcg attgaccgct
     5041 cgcttttgct ctgcgtattc gtcagaaagc agaagttcac gcattgcgcg aaccttgtct
     5101 ttaatcgttt tgaccggctt cgcttcgacg atagcaggtg acgcaacgcg aacctctttc
     5161 gtcaccggag cagcaggctg tgatgcggcg gaaaatttca acatacgccg taaaatgtcg
     5221 gatgcgctct cgccgatatg cttagtgtgg ctggcaatat agctgtagag ttcatcatca
     5281 acttcaatcg ttttcatctt aatccagtgc agtgtcttag ctgaatacaa gtcgccaaga
     5341 gtatatgaat aaatcccggc agcggatagc gtcaagcctg gcttgcagaa aaactcggaa
     5401 taaaccttat ttgttgcagc cctgtggcac aatggtcaac ataataccct aacccgacaa
     5461 cgcgaaaaaa agaactttgc catgaaattg aatatccgcg cgcaaactgc acaaaaccag
     5521 cacaataatt ctcccatcgt tcttgtccat ggtctgtttg gtagcctcga caaccttggc
     5581 gtactggctc gcgatctggt aaacgatcac aatatcatcc aggttgatat gcgtaaccac
     5641 ggtctttcac cgagagatcc ggtaatgaat tacccggcga tggcgcagga tcttgttgat
     5701 actctggatg cacagcagat cgacaaagca acatttatcg gtcactccat gggcggtaaa
     5761 gcggtaatgg cacttacggc actggcccgc gatcgcatcg ataaactggt ggcgatcgat
     5821 atcgcaccgg tcgactatca cgtacgccgt catgatgaga ttttcgcggc tatcaacgcg
     5881 gtcagtgaat cggacgcaca aactcgccag caagcagcag caataatgcg ccagcatctt
     5941 aatgaagaag gggtgattca gtttctgttg aaatcttttg ttgacgggga gtggcgcttt
     6001 aacgtgccgg tattgtggga tcagtatccg catattgtgg gttgggagaa aatcccggca
     6061 tgggatcacc ctgccctgtt tatccctagc ggcaattctc cgtatgttag cgagcagtac
     6121 cgtgatgatt tactggcaca atttccacag gcacgggcgc atgtgattgc aggcgcgggt
     6181 cactgggtcc atgctgaaaa accggatgcg gtattacgcg ctatccgtcg ctatctcaat
     6241 gattaacgca actgattaaa aacccagcgt ccgcgtcgct ttccgcgcgt gggcgttggc
     6301 gtgactcatc gcgctgatgt attatggcgc gctatccatc cgggcagagg cctggctgat
     6361 tgtttccccc gaagtcacca agatcatggc caaagaacaa acggaccgta cgacattaga
     6421 tctgttcgcg cacgagcgtc gaccgggacg accgaaaact aatccgcttt cgcgcgatga
     6481 acagctgcgt attaataaac gcaaccagct aaaacgcgac aaagtacgtg gccttaagcg
     6541 tgtcgaactg aagctgaacg cggaagctgt cgaggcgctg aacgagctgg cggagtcgcg
     6601 caatatgagc cgtagcgaac tgatcgaaga gatgttaatg cagcaactgg cggcgctgcg
     6661 tagtcagggc atcgtttaaa tttccacttt catgtagcac agtgtgcagt cctgctcgtt
     6721 tgctgattcc gaaagactgc ctgttctgct atgattgcct ttatccgtgg gcaattttcc
     6781 acccccattt caataagttt caagaggtta tttcactcat ggctatcact ggcatctttt
     6841 tcggcagcga caccggtaat accgaaaata tcgcaaaaat gattcaaaaa cagcttggta
     6901 aagacgttgc cgatgtccat gacattgcaa aaagcagcaa agaagatctg gaagcttatg
     6961 acattctgct gctgggcatc ccaacctggt attatggcga agcgcagtgt gactgggatg
     7021 acttcttccc gactctcgaa gagattgatt tcaacggcaa actggttgcg ctgtttggtt
     7081 gtggtgacca ggaagattac gccgaatatt tctgcgacgc attgggcacc atccgcgaca
     7141 tcattgaacc gcgcggtgca acgatcgttg gtcactggcc aactgcgggc tatcatttcg
     7201 aagcatcaaa aggtctggct gatgacgacc actttgtcgg tctggctatc gacgaagacc
     7261 gtcagccgga actgaccgct gaacgtgtag aaaaatgggt taaacagatt tctgaagagt
     7321 tgcatctcga cgaaattctc aatgcctgat gtgatgcggc gtagactcat gtctacgccg
     7381 tattaataga taatgccaat caaaataatt gctacaaatt tgtaactttt gctgttgtac
     7441 ctgtacaatg tcccggtgtt caagtggcct tgccgttgta aatgtaagct gtgccacgtt
     7501 tttattaaca atatttgcca gggacttgtg gttttcattt aggcgtggca attctataat
     7561 gatacgcatt atctcaagag caaattctgt cacttcttct aatgaagtga accgcttagt
     7621 aacaggacag attccgcatg actgataaca ataccgccct aaagaaagct ggcctgaaag
     7681 taacgcttcc tcgtttaaaa atcctggaag ttcttcagga gccggacaac catcacgtca
     7741 gtgcggaaga tttatacaaa cgtctgatcg atatgggtga agaaattggt ctggctacgg
     7801 tatatcgcgt actgaaccag tttgacgacg ctggtatcgt cacccgccac aattttgaag
     7861 gcggtaaatc cgtatttgaa ctgacacagc aacatcacca cgatcacctg atctgcctcg
     7921 actgcggcaa ggttatcgaa tttagtgatg attccatcga agcgcgtcag cgtgaaattg
     7981 ccgcaaaaca tggcattcgc ctgactaacc acagtctcta tctttacggt cactgtgccg
     8041 aaggcgattg ccgcgaagat gaacatgcgc acgaaggcaa ataagccagc ctgaatgaga
     8101 aaaagccaac ctgcgggttg gcttttttat gcaagggaaa taagaaaagc gagagttaca
     8161 gctctcactt atttgttatt actgcgaatt tcctgccaaa ctttatcaca atcggctttc
     8221 accgcctgat catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgg
     8281 acactttcct ggtcgggaaa atgctgatgt tgtttctctt tcttcagcac gtttaacacg
     8341 ctctggcagg cttcaatttt ttctggcgaa ccttgtgcgg tgttgataca ggcgctgtat
     8401 gcctctttca gacgactgtc ttcctgtggc gcggttgatt gcgcacaagc taccagcccc
     8461 gatgccatta tggcgatgag aatcagtttt ttcatagcaa attcctcaaa cgtgcggcag
     8521 gcacaaaacc tgccgcgtcg ggcatcagaa gatggtgaat ggtgcgatta ccataaattt
     8581 cacgtcacgc tcatcctgga agatgttgcc gtaaccaccg ccccagcttg ggatgtcgga
     8641 gtggttgtcg tattcggtga agtgcagttt gaacatcgtg cctttggcgc gaccgtcctg
     8701 aatggtgtag accgcatcca ggctgtatgc agactcttca atagtccggt ttttgtcgta
     8761 gtacgcatcc ggattgctct gccaggtcgc aggtttagca tcccatgcgt aaacgtagga
     8821 agcgccgatg gcgaagcctg gaagattcca gtttttcagg tcatacatcg caccgaagaa
     8881 gaccgctttt tcgccgttag cgttgaagtc ggaacggtta tcccaccaga tatccaggcg
     8941 gccgttggag gaagcgtagg ctggagtcat acgttgcagg aagtatccct gctgaccgtc
     9001 agccttaacc caggtgcctt cgaggcgcaa atccactacg tcagccgccc ggtaaccaaa
     9061 ggtcaacgct tgcagccagg cggtgccgtc ataaaggtcg ttgacgctgc gatcgtcaac
     9121 tttatcgcgc gtaccataga actggtagct ggtggttaac gggctaccgg cgatatcaaa
     9181 tttgtagctg gctttggcaa aatattgatc gatataccct tccgcctgac caaatgccgc
     9241 ttccagtacg aagttatttt tgaagtcgta tttcgcccca agggagtgca gataatcaac
     9301 tttggtggtt ttatcgttct gataaaactc atccatttcc agatgccacg gcgctttgta
     9361 ttcgttggtc cacatgtagg agaaactcaa cgcaccagca tcgccgtaat caaaattcgc
     9421 cccggcttcc gccccctgat aagtacctgg cataaagctc cagtgcggcg ctaacagcgt
     9481 ttgaccagtt ggctgaatgt aacctgccct cgcccaaacc ggaccgtatt taaatttggc
     9541 cgcagcttta tacaggctta taccgctttt gtcgccagac cagtcttcgt cataggcttt
     9601 attacttttt gaaaacgcga tttcgttcgg gtgggagctg tcgccgtttt ccgccatttc
     9661 aatcgccgta aacacagcaa tatcaaggcc gaacatatca gcagcataac cggactgaaa
     9721 atcgaggttg gcattccagg tggagtgaga aaggttggtt ttgtatttgt cgccgtcggt
     9781 aacatcttta cggtcgcgtt cacgctgcca gtaatatata ccgccggtta aggttgaatc
     9841 gtcgatgaat ccttcggcgc gagcctccgg cattgccata aagcccgaca ttgctgtaac
     9901 accggcgata gccagcgcca gcgtactacg tttgccacta aacgtacgca tgggttaatc
     9961 ctctttgacg tataaattgc tgcaccaaag gtgaacagcg aaaaaaataa aaattaaaaa
    10021 agtagcattg agaaaagtct cgctgactac aggaatagac tatttttcgc gacgcgaatt
    10081 atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata ttttgatgat
    10141 ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa agcgtaaatt
    10201 tccagcaaga tgaaagcggt tacattttat ttaattatgg ttt
//
LOCUS       DAJTOR010000148        10232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTOR010000148 DAJTOR010000000
VERSION     DAJTOR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.148"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(28..1926)
                     /gene="espL1"
                     /locus_tag="OO249_003261"
     CDS             complement(28..1926)
                     /gene="espL1"
                     /locus_tag="OO249_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCR5637447.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSELINQFARQLITLLD
                     TSDLSSMLSYCITQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAC
                     FLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQLVYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPVGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLIYNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDETIDIFLNALTTPIAQELLNKKIVMDILAMKTRDGELGLFAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAHGTPVLYIAMSKGNEDVVLSYISTL
                     NIFAKKYSFSQRQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     ADELKTYL"
     gene            2448..4376
                     /gene="thrS"
                     /locus_tag="OO249_003262"
     CDS             2448..4376
                     /gene="thrS"
                     /locus_tag="OO249_003262"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5637448.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4380..4922
                     /gene="infC"
                     /locus_tag="OO249_003263"
     CDS             4380..4922
                     /gene="infC"
                     /locus_tag="OO249_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5637449.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQRVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            5019..5216
                     /gene="rpmI"
                     /locus_tag="OO249_003264"
     CDS             5019..5216
                     /gene="rpmI"
                     /locus_tag="OO249_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5637450.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5269..5625
                     /gene="rplT"
                     /locus_tag="OO249_003265"
     CDS             5269..5625
                     /gene="rplT"
                     /locus_tag="OO249_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5637451.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5742..5866
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5748..5792
                     /gene="pheM"
                     /locus_tag="OO249_003266"
     CDS             5748..5792
                     /gene="pheM"
                     /locus_tag="OO249_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5637452.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6075..7058
                     /gene="pheS"
                     /locus_tag="OO249_003267"
     CDS             6075..7058
                     /gene="pheS"
                     /locus_tag="OO249_003267"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5637453.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7073..9460
                     /gene="pheT"
                     /locus_tag="OO249_003268"
     CDS             7073..9460
                     /gene="pheT"
                     /locus_tag="OO249_003268"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5637454.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9465..9764
                     /gene="ihfA"
                     /locus_tag="OO249_003269"
     CDS             9465..9764
                     /gene="ihfA"
                     /locus_tag="OO249_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5637455.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(10068..10208)
                     /locus_tag="OO249_003270"
     CDS             complement(10068..10208)
                     /locus_tag="OO249_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR5637456.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT         2701 a         2307 c         2615 g         2609 t
ORIGIN      
        1 taagttggca gcattaccaa tagttggtta taaatacgtc tttagttcat cagcactaaa
       61 actcaggctt tggctgatta catttatagc agcataatat gtttctacag ttttataatg
      121 attatgatga atggctatat gaacagctga catgttgtca tgatttttag cggccaacag
      181 tgtgaataac tgacgttgac taaaagaata tttttttgca aaaatattca gcgtcgatat
      241 ataagataac acgacgtctt cattaccctt gctcatggcg atgtataata caggggttcc
      301 atgtgctgta gcgcctttta ataaatccat gatgttagct ttgctcaact tgtatttaaa
      361 ggcgatgcca ttaattttag agaggaaccg tgtgacacac aaagggtgat tattttccat
      421 tgcagcaaac aaccctagtt caccatcacg tgttttcatt gctaaaatat ccatcactat
      481 ttttttgttt aaaagctctt gtgctattgg agtggttaat gcattcagaa agatatcgat
      541 tgtttcatcg aacccataca acatggagat atagagccca ggtgtttcgt tagcactttt
      601 agtttgcagt aatttaacca gattatcttc atgaatgata tgattgtata tcagtgactg
      661 aatttcttgc ataaatattt ttacgttgtc agtatgtccg ttcatcatag ccagaaataa
      721 agcaggtgta ccaacgctat tctgcgcagt tattatttca atgatttctt tttcagggag
      781 tatgggcaac tgtttgaata atgtaataag ctgttgagtc tggcctgtgg ccattattaa
      841 caacattgcc gatggatgta taggggcaaa tggcattcgt gatgcaatac ccgcgaaaat
      901 agcccctgga actcccacag gtaatagggg cagaatagca attgcacagt tgcgaacata
      961 cctatcagcc caggtctgag catcgggacc tgaactgatg aacttatcaa gcgaaagaaa
     1021 gccccgtgct gtcctgtggg tgcctctgac ggcaacgttg gtatcgttcg ggtcgtagac
     1081 ggacactaca tagtttaatt gtccatacac taactgcttt attcgtagcc ttactgacaa
     1141 agtatgattg tctatatata aaatcgcgga catatgactg acattgtttg ccatcattcg
     1201 acaaaagaaa atatctaaag cgaagccaaa atccttactc gcgataacgc gactccctgc
     1261 aggaggatgg cgaagtaatg ctctgaactc cgcatcccgg actaagggga catgctgctg
     1321 tatggattca acggaggagt aatgtttaat caggttgact ttgccttttc catttctcaa
     1381 tgaatcaagg gcgtattgca atgacagatg gcgacaaaaa atagcgtcag aaggagattg
     1441 caaaaaacat gcactaccat tgagatttac tgttggttgt atagtttttt ctctatggat
     1501 ttttttagac agtttcatac aatggttacg atcttcggca gttaactgtt cctgctcaat
     1561 ataatccagt atgcttcgca aatggttagc cttaataggc gtggtttcca gaaataaggg
     1621 aatgatttct tttttatagt gtaatgtaat aagtatattt tctgcatgga tgggtgaagt
     1681 ggcaaagtta atgataaaaa gggcagtgga gtaacaattt tgtgatattt ttcgacagtt
     1741 tgcggtaaat tcctgagtaa tacagtatga cagcatcgaa cttaagtcag aggtatcaag
     1801 tagggtgatg agttggcgcg caaattgatt aataagctct gatgaaggcg ttggtatgtc
     1861 gctatcgatg tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg
     1921 actcataatc tattcacctt atatatactg gaggttttga ggatgatgat taattattat
     1981 cgccagaacg aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg
     2041 atatgaccac ttcgtcatta atctcatgat cgtttaaata ctaccgccaa atcacgcaac
     2101 gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac ctttcagacg
     2161 caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact acaaacgaat
     2221 tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag tgttaacatc
     2281 gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg attgcgaacc
     2341 aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca aatttttctt
     2401 tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg cctgttataa
     2461 ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg gatgttgcgc
     2521 tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat ggcgaactgg
     2581 ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc gccaaagacg
     2641 aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat gcgattaaac
     2701 aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt ttttattacg
     2761 acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag aagcggatgc
     2821 atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg cacgcagcgc
     2881 gtgaaacttt cgccaaccgt ggggagagct acaaagtctc cattcttgac gaaaacatcg
     2941 cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg tgccgcggtc
     3001 cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg gcaggggctt
     3061 actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg gcgtgggcag
     3121 acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa cgcgaccacc
     3181 gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg ggtatggtat
     3241 tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt cgttctaaac
     3301 tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt gtcctgtggg
     3361 aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct tctgagaacc
     3421 gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc aaccaggggc
     3481 tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc caccgtaacg
     3541 agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccaa gatgacgcgc
     3601 atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc cgtttagtct
     3661 atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc actcgtcctg
     3721 aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg gcggttgcgc
     3781 tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc tacggtccga
     3841 aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt acagtacagc
     3901 tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac aacgagcgta
     3961 aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggaacgtttc atcggtatcc
     4021 tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag gttgttatca
     4081 tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa ctatcaaatg
     4141 cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa atccgcgagc
     4201 acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg gaatcaggca
     4261 aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta aatgaagtga
     4321 tcgagaagtt gcaacaagag attcgcagcc gcagtcttaa acaattggag gaataaggta
     4381 ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc gaaattcgcg
     4441 cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg agtctgagag
     4501 aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc cctaacgccg
     4561 agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag agcaagtctt
     4621 ctaaggaaca gaagaaaaag caaagagtta tccaggttaa ggaaattaaa ttccgtcctg
     4681 gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt ctcgaagagg
     4741 gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac cagcaaatcg
     4801 gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagtg gtcgaatcct
     4861 tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag aagaaacagt
     4921 aaggccttca agtagcactg tctgtggagc cttcgggctt cacaggcttt gttcgccttt
     4981 gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt aagaccgtac
     5041 gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac aagcacgcta
     5101 acctgcgtca cattctgacc aaaaaagcga ccaaacgtaa acgtcacctg cgtccgaaag
     5161 ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac gcataagccg
     5221 ttaacgtttt taacttttta attagaatat agatacagga gagcacatat ggctcgcgta
     5281 aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc taaaggctac
     5341 tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa agctggtcag
     5401 tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg gattgcacgt
     5461 atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa tggcctgaaa
     5521 aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt cgacaaagta
     5581 gcgttcaccg ctctggttga aaaagcgaaa gcagctctgg cataagccag ttgaaagagg
     5641 gagcttgtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta tcgccgtagc
     5701 cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg aatgctgcta
     5761 ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg tgagaagaga
     5821 aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc gcgttttgaa
     5881 aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggatac ggccagcgcg
     5941 gagaaaccgg agcgtattta agtacgtgag aatttcgagc actgcccggg accaaaatgg
     6001 caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc accactgaca
     6061 caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag gcggccatta
     6121 gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg ggtaaaaaag
     6181 ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag cgtccggcag
     6241 ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat gcgcgtaaag
     6301 cggaactgga aagcgcagcg ctgaatgcgc gtctggcggc ggaaacgatt gatgtctccc
     6361 tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc atcgaccgta
     6421 tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa atcgaagacg
     6481 attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc gctgaccacg
     6541 acactttctg gtttgacact acccgcctgc tgcgtaccca gacctctggc gtacagatcc
     6601 gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgc gtttatcgta
     6661 acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg attgttgata
     6721 ccaacatcag ctttaccaac ctgaaaggca ctctgcacga tttcctgcgt aacttctttg
     6781 aggaagattt gcagattcgc ttccgtcctt cctacttccc gttcaccgag ccttctgcag
     6841 aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggttgc gggatggtgc
     6901 atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt ttcgccttcg
     6961 ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg cgttcattct
     7021 tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga ttatgaaatt
     7081 cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgatg cgctggcgaa
     7141 tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg gtagcttcca
     7201 cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg acaaactgcg
     7261 tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgtg gtgcgccaaa
     7321 ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc cgggtgattt
     7381 caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt gctccttctc
     7441 tgaactggga atttctgacg atcacagcgg cattatcgaa ctgcctgcag atgcgccgat
     7501 tggcactgac atccgcgaat acctgaaact cgatgacaac accatcgaaa tcagcgtaac
     7561 gccaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg ccgtgctgaa
     7621 tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg acgacacgtt
     7681 gccgattaca gtcgaagcgc ctgaagcctg cccgcgctat cttggccgtg tggtaaaagg
     7741 cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc gttgcgggat
     7801 ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac tgggccagcc
     7861 gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga tggcaaaaga
     7921 gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg acactctggt
     7981 catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg aacactctgg
     8041 cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc cgctgtctat
     8101 caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt atgagcgtgg
     8161 cgttgatccg gcactgcagc acaaagcgat ggaacgtgcg acccgtctgc tgatcgacat
     8221 ctgcggtggt gaggctggtc cggtaattga tatcaccaac gaagcaacgc tgccgaagcg
     8281 tgcaaccatc actttacgtc gtagcaaact ggattgcctg atcggccatc atattgctga
     8341 tgaacaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag gcaaagacga
     8401 gtggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag atctggtcga
     8461 agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac aggcaagcct
     8521 gattatgggt actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa cgctgctcaa
     8581 cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag tgcagcagat
     8641 gatccatcca ggcgttgaag ccttactgct gccaagcccg atctctgttg aaatgtcagc
     8701 aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc agaaccgtca
     8761 gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata ctcaggcacc
     8821 gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc gttacgaaga
     8881 gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg atcttgaatc
     8941 cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag cgaatccggc
     9001 actgcatccg gggcaatccg cagcgattta tctgaaaggg gaacgtattg gttttgttgg
     9061 agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc tggtgttcga
     9121 actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga tttctcgctt
     9181 cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg cagcggatat
     9241 tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact tatttgacgt
     9301 gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc tgatcctgca
     9361 agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca aatgtgtaga
     9421 ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg cttacaaaag
     9481 ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat gccaaagaac
     9541 tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa caggtgaaac
     9601 tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga cgtaacccga
     9661 aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga cccgggcaga
     9721 agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat ctaactaaaa
     9781 aggccgctct gcggcctttt ttctttcaat aactaattga tttataatgt tttatatctc
     9841 ttcgttccac gatgcgtcca catttagtgg ggggagggga gtaagcccgc tacattgtgg
     9901 gctttgtctt atagtctgat cactgtttca agtcggtgag gacaccaaaa agaacccccg
     9961 cagtttttac gctgcgggtt tgttgttcat gtctgtgaga tagggtgcct tatcgacctt
    10021 accctggcaa ccgattgacg ggggattgct cccccgtcgc ggtttcctta ctgcttacac
    10081 tgtaagaacg ccgcaaactc cgctccccag aagctcatcc gtatttcgca cagcgaaccg
    10141 tgcagcatcc agatgatgag gattgccgtc acgcagaacg tgatggccgt aagcgatttt
    10201 tgcgacatag cgcttgctcc tttttcgggg ag
//
LOCUS       DAJTOR010000149        10215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTOR010000149 DAJTOR010000000
VERSION     DAJTOR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.149"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            143..1999
                     /gene="kup"
                     /locus_tag="OO249_003271"
     CDS             143..1999
                     /gene="kup"
                     /locus_tag="OO249_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5637457.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSLTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2166..2585
                     /gene="rbsD"
                     /locus_tag="OO249_003272"
     CDS             2166..2585
                     /gene="rbsD"
                     /locus_tag="OO249_003272"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5637458.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2593..4062
                     /gene="rbsA"
                     /locus_tag="OO249_003273"
                     /pseudo
     CDS             2593..4062
                     /gene="rbsA"
                     /locus_tag="OO249_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            4067..5032
                     /gene="rbsC"
                     /locus_tag="OO249_003274"
     CDS             4067..5032
                     /gene="rbsC"
                     /locus_tag="OO249_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5637459.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5057..5943
                     /gene="rbsB"
                     /locus_tag="OO249_003275"
                     /pseudo
     CDS             5057..5943
                     /gene="rbsB"
                     /locus_tag="OO249_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6069..6998
                     /gene="rbsK"
                     /locus_tag="OO249_003276"
     CDS             6069..6998
                     /gene="rbsK"
                     /locus_tag="OO249_003276"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5637460.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7002..7994
                     /gene="rbsR"
                     /locus_tag="OO249_003277"
     CDS             7002..7994
                     /gene="rbsR"
                     /locus_tag="OO249_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5637461.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(7960..9387)
                     /gene="mdtD"
                     /locus_tag="OO249_003278"
     CDS             complement(7960..9387)
                     /gene="mdtD"
                     /locus_tag="OO249_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5637462.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     RATWHWIFLINIPIGIAGLLYARKHMPNFTTAQRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9410..10102)
                     /locus_tag="OO249_003279"
     CDS             complement(9410..10102)
                     /locus_tag="OO249_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5637463.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KTCQALLRSPDK"
BASE COUNT         2487 a         2458 c         2702 g         2568 t
ORIGIN      
        1 caaagggggg tatttttctt tgagccaggt taatgtggcc gcatttagga gtacgatttt
       61 gccgttaatc gtgcatactg tgcgcttttt tgtgggccaa gggactaagc acacatttca
      121 tatttcaacg aaagactagt ctatgagcac tgataataag caatcattgc ccgcgattac
      181 cctcgcggcg attggagttg tctacggcga tattggtacc agcccgttat atacacttcg
      241 tgaatgtttg tccggccagt ttggttttgg cgttgaacgc gatgccgtgt ttggcttttt
      301 atcgctgatc ttctggctgc taatctttgt ggtttccatt aaatatctca ccttcgtgat
      361 gcgggcagat aacgccggtg agggggggat cctgacgttg atgtcgcttg ccgggcgtaa
      421 tacgtcggcg cgaaccacat caatgctggt gattatgggg ctaatcggcg gcagcttttt
      481 ctatggtgaa gtcgtcataa cacccgctat ttcggtgatg tcagccattg aaggtctgga
      541 aatcgtcgcc ccgcagctgg atacctggat agttcccctc tcaattatcg ttctcacttt
      601 attatttatg attcaaaaac atggcacggc tatggtcggt aagctgtttg cgccgatcat
      661 gctgacctgg tttttgattc tggcaggact ggggttacgt agcattattg ctaacccgga
      721 agtgctgcat gcgctgaatc caatgtgggc ggtgcatttc ttccttgaat acaaaacggt
      781 ttcttttatt gcattagggg cagtggtgtt gtcgattacg ggggtcgagg cgctgtatgc
      841 tgatatgggg cactttggta agttccctat tcgcctggcg tggttcaccg tcgtattacc
      901 ttccttgacc cttaattact tcggtcaggg agcgctgttg ttaaagaacc cggaagcgat
      961 taagaacccg ttcttcctgt tggcaccgga ctgggcgctg atcccgctgc tgatcatcgc
     1021 cgcactggcg acggtaattg cctcgcaggc ggttatctct ggcgtcttct cattgacgcg
     1081 tcaggcggta cgtctgggat atttgtcgcc gatgcgcatt attcacacct ccgaaatgga
     1141 gtcagggcaa atctatattc cgtttgtgaa ctggatgctc tatgtcgcgg tcgtgattgt
     1201 gattgtcagc tttgagcact ccagcaacct ggcggcggcg tacgggattg cggtgaccgg
     1261 aaccatggtg ctgacgtcta ttctctcgac taccgtggca cgtcagaact ggcactggaa
     1321 taagtatttt gttgcgctga tcctgattgc tttcctttgt gtcgatattc cattgttcac
     1381 cgctaacctc gataaactgc tctccggcgg ttggttgcca ttgagcctcg gtacggtgat
     1441 gtttatcgtg atgaccacct ggaagagcga gcgtttccgc ttgctgcggc ggatgcatga
     1501 acatggtaac tctctggaag cgatgattgc ttcgctggag aaatcaccgc ctgttcgcgt
     1561 gcccgggacc gcggtgtata tgtcgcgtgc aatcaacgtc attccctttg cgctgatgca
     1621 taaccttaaa cataacaagg tattgcatga gcgggtgatt ctgttaactc tgcgtaccga
     1681 agacgcgcca tatgtccata atgtccgtcg ggtacagatt gaacaactgt cgctcacttt
     1741 ctggcgcgtg gtggcaagtt atggttggcg agaaacgcca aacgtggaag aagttttcca
     1801 ccgctgcggt ctggaaggat taagttgccg gatgatggaa acctccttct ttatgtcgca
     1861 tgagtcgaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc tgctgcaacg
     1921 taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta tcgaactggg
     1981 tactcaggtc gaaatctaac gccagacgcc tcctttctcc ataagggggc gtttttgttt
     2041 tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta acgtcacgat
     2101 ggttttccca actcagtcag gattaaactg cgggtcagcg aaacgtttcg ctgatggaga
     2161 aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc tcccgtctgg
     2221 gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa agtacaacgc
     2281 gtatcgatat ggcattaacc cagggtgtac cttcttttat gcaggtgctg ggcgtcgtta
     2341 caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa cataatccgc
     2401 aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag ggaaatacca
     2461 ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa agtcaggcgg
     2521 taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct ggcgtgacgt
     2581 tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc cttcccgggc
     2641 gtaaaagccc tctcgggcgc tgcgttaaat gtctatccgg gccgcgtgat ggcgctggtg
     2701 ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat ctatgctcgc
     2761 gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc gaaatcttcc
     2821 caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca gttgaccatt
     2881 gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat tgactggaaa
     2941 accatgtatg ccgaagcgga taaattgctg gctaaactta acctgcgctt taaaagcgac
     3001 aagctggtgg gcgatctttc catcggtgac cagcaaatgg ttgaaatcgc caaagtgctg
     3061 agctttgagt cgaaagtcat cattatggat gaaccgaccg atgcgctgac cgataccgaa
     3121 accgaatccc tgttccgcgt catccgcgaa ctaaaatcgc aaggccgcgg tattgtctat
     3181 atctcccacc gcatgaaaga aatcttcgag atttgcgatg ccgttaccgt ttttcgtgac
     3241 gggcaattta ttgctgagcg cgaagtggca tcactgaccg aagattcgct gattgagatg
     3301 atggtgggtc gcaagctgga agatcaatat ccacacctgg acaaagcgcc gggagatatc
     3361 cgcctgaaag tcgataatct ctgcggacct ggcgttaacg atgtctcttt tactttacgc
     3421 aaaggcgaaa ttcttggcgt ctctggtttg atgggcgcag gtcgtaccga actgatgaaa
     3481 gtgctctacg gcgcactacc gcgcaccagc ggttacgtca ccctggatgg gcatgaagtc
     3541 gttacccgtt caccgcagga tggcctggca aacggcattg tgtatatctc cgaagaccgt
     3601 aaacgtgacg gtttagtgtt gggcatgtca gtaaaagaga acatgtcgct gacagcgctg
     3661 cgctacttca gccgcgctgg cggcagtttg aagcatgccg atgaacagta ggctgtgagt
     3721 gatttcattc gtctgtttaa tgtgaaaaca ccgtcgatgg aacaggcaat tggtctgctt
     3781 tccggtggca atcagcaaaa agtgttgatc cttgatgaac ctacccgtgg cgtagatgtc
     3841 ggcgcgaaaa aagagatcta tcaactgatt aaccagttca aagccgatgg cttgagcatc
     3901 attctggtgt catcggagat gccagaagta ttaggcatga gcgatcgcat catcgtcatg
     3961 catgaagggc atctcagcgg ggaatttact cgtgagcagg ccacccagga agtgttaatg
     4021 gctgccgctg tgggcaagct taatcgcgtg aatcaggagt aaaaaaatga caacccagac
     4081 tgtctctggt cgccgttatt tcacgaaagc gtggctgatg gagcagaaat cgcttatcgc
     4141 tctgctggtg ctgatcgcga ttgtctcgac gttaagcccg aactttttca ccatcaataa
     4201 cttattcaat attctccagc aaacctcggt gaacgccatt atggcggtcg ggatgacgct
     4261 ggtgatcctg acttcgggca tcgacttgtc tgtaggttct ctgttggcgc taaccggcgc
     4321 agttgctgca tctatcgtcg gcattgaagt caatgcgctg gtggctgtcg ctgctgctct
     4381 cgcgttaggt gccgcaattg gtgcggtaac cggggtgatt gtagcgaaag gtcgcgttca
     4441 ggcgtttatc gctacgctgg ttatgatgct tttactgcgc ggcgtgacca tggtttatac
     4501 caacggtagc ccagtgaata ccggctttac tgagaacgcc gatctgtttg gctggtttgg
     4561 tattggtcgt ccgctgggcg taccgacgcc agtctggatc atggggattg tcttcctcgc
     4621 ggcctggtac atgctgcatc acacgcgtct ggggcgttac atctacgcgc tgggcggcaa
     4681 cgaagcggca acgcgtcttt ctggtatcaa cgtcaataaa atcaaaatca tcgtctattc
     4741 tctttgtggt ctgctggcat cgctggccgg gaccattgaa gtggcgcgtc tctcctccgc
     4801 acaacccacg gcggggactg gctatgagct ggatgctatt gctgcggtgg ttctgggcgg
     4861 tacgagtctg gcgggcggaa aaggtcgcat tgttgggacg ttgatcggcg cattaattct
     4921 tggcttcctt aataatggat tgaatttgtt aggtgtttcc tcctattacc agatgatcgt
     4981 caaagcggtg gtgattttgc tggcggtgct ggtagacaac aaaaagcagt aataacgact
     5041 acaggacatc ttgaatatga acatgaaaaa actggctacc ctggtttccg ctgttgcgct
     5101 aagcgccacc gtcagtgcga atgcgatggc aaaagacacc atcgcgctgg tggtctccac
     5161 gcttaacaac ccgttctttg tatcgctgaa agatggcgcg cagaaagagg cggataagct
     5221 tggctataac ctggtggtgc tggactccca gaacaacccg gcgaaagagc tggcgaacgt
     5281 gcaggactta accgttcgcg gcaccaaaat tctgctgatt aacccgactg actccgacgc
     5341 agtaggtaat gctgtgaaga tggctaacca ggcgaacatc ccggttatca ctcttgaccg
     5401 ccaggcaacg aaaggtgaag tggtgagcca cattgcttct gataacgtac tgggtggcaa
     5461 aatcgccggt gattacatcg cgaagaaagc gggtgaaggt gccaaagtta tcgagctgca
     5521 aggcattgct ggtacatccg cagcccgtga acgtggcgaa ggcttccagc aggccgttgc
     5581 tgctcacaag tttaatgttc ttgccagcca gcagattttg atcgcactaa aggtttgaac
     5641 gtaatgcaga acctgttgac cgctcatccg gatgttcagg ctgtattcgc gcagaatgat
     5701 gaaatggcgc tgggcgcgct gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc
     5761 gtcggatttg acggtacacc ggatggcgaa aaagcggtga atgatggcaa actagcagcg
     5821 actatcgctc agctacccga tcagattggc gcgaaaggcg tcgaaaccgc agataaagtg
     5881 ctgaaaggcg agaaagttca ggctaagtat ccggttgatc tgaaactggt tgttaagcag
     5941 tagttttaat caggttgtat gacctgatgg tgacataaat acgtcatcga cagatgaacg
     6001 tgtaatataa agaaaagcag ggcacgcgcc accctaacac ggtggcgcat tttatggaca
     6061 tcccgaatat gcaaaacgca ggcagcctcg ttgttcttgg cagcattaat gctgaccaca
     6121 ttcttaatct tcaatctttt cctactccag gcgaaaccgt aaccggtaac cactatcagg
     6181 ttgcatttgg cggcaaaggc gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata
     6241 tcgcgtttat tgcctgtacg ggtgatgaca gcattggtga gagcgttcgc cagcagctcg
     6301 ccactgataa cattgatatt actccggtca gcgtgatcaa aggcgaatca acaggtgtgg
     6361 cgctgatttt tgttaatggc gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg
     6421 ctgccctttc cccggcgctg gtggaagcgc aacgtgagcg tattgccaac gcgtcagcat
     6481 tattaatgca gctggaatca ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc
     6541 aaaataagac tatcgttgcg cttaacccgg ctccggctcg cgaacttcct gacgaactgc
     6601 tggcgctggt ggacattatt acgccaaacg aaacggaagc agaaaagctc accggtattc
     6661 gtgttgaaaa tgatgaagat gcagcgaagg cggcgcaggt actgcatgaa aaaggtatcc
     6721 gtactgtact gattacttta ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc
     6781 agcgcgttcc tggattccgg gtgcaggctg tcgataccat tgctgccgga gataccttta
     6841 acggcgcgtt aatcacggcg ttgctggaag aaaaaccatt gccagaggcg attcggtttg
     6901 cccatgctgc agctgcgatt gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc
     6961 gtgaagagat cgacgcattt ttagacaggc agaggtgacg cttggctaca atgaaagatg
     7021 ttgcccgcct ggcgggcgtt tctacctcaa cagtttctca cgttatcaat aaagatcgct
     7081 tcgtcagtga agcgattacc gccaaagttg aagcggcgat taaagaactc aattacgcgc
     7141 catcagccct ggcgcgtagc ctcaaactca atcaaacaca taccattggc atgttgatca
     7201 ctgccagtac caatcctttc tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg
     7261 aacgcggtta tagtctcgtc ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca
     7321 atctggaaac gctgatgcaa aaacgcgttg atggcttgct gttactgtgc accgaaacgc
     7381 atcaaccttc gcgtgaaatc atgcaacgtt atccgacagt gcctactgtg atgatggact
     7441 gggctccgtt cgatggcgac agcgatctta ttcaggataa ctcgttgctg ggcggagact
     7501 tagcaacgca atatctgatc gataaaggtc atacccgtat cgcctgtatt accggtccgc
     7561 tggataaaac tccggcgcgc ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg
     7621 gtctcaacat tcctgatggc tatgaagtca ctggtgattt tgaatttaac ggcgggtttg
     7681 acgccatgcg ccaactgtta tcacatccgc tgcgtcctca ggccgtcttt accggaaatg
     7741 acgctatggc tgttggcgtt taccaggcgt tatatcaggc agagttacag gttccgcagg
     7801 atatcgcggt gattggctat gacgatatcg aactggcaag ctttatgacg ccaccattaa
     7861 ccactatcca ccaaccgaaa gatgaactgg gggagctggc gattgatgta ctcatccatc
     7921 ggataaccca gccgaccctt cagcaacaac gattacaact tactccgatt ctgatggaac
     7981 gcggttcggc ttagatttac gctgtctttt gatcaaatta ttaccatcgg ttgttttcag
     8041 aagcatgaac attgctgctg aagcaacagt aataatgccc atcgtgataa acgtatagtg
     8101 gaattgttcg acagtcgttg tgccttccat cccttcataa acgcgaagga cggccgcact
     8161 tacagcaacg cctaaactaa tcgacagttg ctgcgtgacc gccagaacac tgttaccgct
     8221 gctggcgttg tcatcggtca gatcggcaag tgtgatggta ttcatcgcgg taaattgcgt
     8281 cgacatagcc atccctaata taaacaacgg caagatcagc atccatatcg ccattgccgg
     8341 tgattgcaaa gagaactgag cgatcattag cccaataatc accgtgatcc ccactaacgt
     8401 atggcgatag cccagacgac gtaagacttg ggtaaccatc gattttgcaa taatggaacc
     8461 taacgctgtc ggtgccatca tacagccggc aataaacgcc tgataaccaa atcctacctg
     8521 taacatcaat ggcataagga acggtacact accggtcccc agacgggttg caatattgcc
     8581 tacgataccg atcgagaaag tgcgggtttt aaataaatct aatgaaatta atgggtttgg
     8641 cgtgtgtcgc gcatggagaa tatagagaag cagtaacccg atgctggtga caattaccgt
     8701 caaggcaatc cagctggcga caatcttttc cccgaatagc tctattccgc ttgagaagag
     8761 aacaaggctg aggccaaaca gcaaaaagcc agtgatatcg aatctgcgtt gtgcggtggt
     8821 gaaattgggc atatgtttgc gcgcgtaaag aaggcccgca atacctatgg ggatattgat
     8881 taaaaatatc cagtgccagg ttgccctggt gaccagcacg ccgccaagaa cggggcctaa
     8941 aattggcccc accagacccg gcatggcgac aaaattcaat acaggaagaa gttcattacg
     9001 aggataagca cgcagtaagg ccagccgagc aacaggcatc atcattgcgc cgcctatccc
     9061 ctgaataacc cggaagacaa ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga
     9121 acccaacgtg aacagactca cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc
     9181 tagccatccg cttaccggaa taaacatcgc caccgtcagc gtataactga tgatggctga
     9241 ttgcatcgcg agaggagaac gattaaggct atgagcgatt gcgggtaagg cggtattcag
     9301 aatagtggca tcaagtgcct gcatgaagaa ggccatcgcc gcgatccacg gcaaacccgc
     9361 catactacgc ttctttttat cgctcattca atgtcctgtt atcgggttat cacttatcag
     9421 gtgagcgtag cagcgcctga caagttttaa atgccgcgtc gccatcgctt tggataatcg
     9481 catcgacaat cgcctggtgc agatccagct ttatcactgt gtcgctggta attgacgtga
     9541 agtaagtgtg ataaaccgaa tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc
     9601 tcatttcata gatatgctca tgccaggcca tatcgacttc gatccagcgt tcacggcgaa
     9661 agttcttttt taatgccgcc atttcggcca ttaacgtatt gagatgcgcc ttctgttccg
     9721 cggtgccaac cgttgctgcc agtaggcagg cttgcggctc cagacagata cgcataacca
     9781 gaaagtgatc gatgacctga tgaaagttct cttctgtcat ccaccaggta agcaattcct
     9841 gatcaagaaa attccagttt gattgtggca tgacccgggt accaattcgc ggtcgcggta
     9901 aaaccatccc ttttgccgtt aacgttttga ccgcttcgcg taccgctgta cgactcactc
     9961 caaattgctc gcccagctca atctcaccag gcaaaatggt gccgggttca tattcacctt
    10021 ttaagatgcg ttgcgccagc ttctcagcca gaacatacga aaggtttttc tgtgcagcta
    10081 actgttgtgc gcttaaaggc attactaatc ttcctttttc tttttattcc tccttagtat
    10141 gccaccagga agtgtgatta cggttgcaaa aacggcaaat tgcttgtttt atggcacatt
    10201 aacggggctt ttgct
//
LOCUS       DAJTOR010000150        10156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTOR010000150 DAJTOR010000000
VERSION     DAJTOR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.150"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(27..1022)
                     /gene="gapA"
                     /locus_tag="OO249_003280"
     CDS             complement(27..1022)
                     /gene="gapA"
                     /locus_tag="OO249_003280"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5637464.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTDAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1364..1777
                     /gene="msrB"
                     /locus_tag="OO249_003281"
     CDS             1364..1777
                     /gene="msrB"
                     /locus_tag="OO249_003281"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5637465.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1819..2091
                     /locus_tag="OO249_003282"
     CDS             1819..2091
                     /locus_tag="OO249_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5637466.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2252..2893)
                     /gene="pncA"
                     /locus_tag="OO249_003283"
     CDS             complement(2252..2893)
                     /gene="pncA"
                     /locus_tag="OO249_003283"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5637467.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2904..3920)
                     /gene="ansA"
                     /locus_tag="OO249_003284"
     CDS             complement(2904..3920)
                     /gene="ansA"
                     /locus_tag="OO249_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5637468.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4087..5943)
                     /gene="sppA"
                     /locus_tag="OO249_003285"
     CDS             complement(4087..5943)
                     /gene="sppA"
                     /locus_tag="OO249_003285"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5637469.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDHNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPSAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6104..6655
                     /locus_tag="OO249_003286"
     CDS             6104..6655
                     /locus_tag="OO249_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5637470.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAILEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6772..7815
                     /gene="selD"
                     /locus_tag="OO249_003287"
     CDS             6772..7815
                     /gene="selD"
                     /locus_tag="OO249_003287"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5637471.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQACVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7820..9781
                     /gene="topB"
                     /locus_tag="OO249_003288"
     CDS             7820..9781
                     /gene="topB"
                     /locus_tag="OO249_003288"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5637472.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            9908..>10156
                     /locus_tag="OO249_003289"
     CDS             9908..>10156
                     /locus_tag="OO249_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637473.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMM"
BASE COUNT         2547 a         2485 c         2687 g         2437 t
ORIGIN      
        1 tttttagatc acagtttcat ctcaacttat ttggagatgt gagcgatcag gtccagaact
       61 ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa gttgtcgttc
      121 agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt gaaatcggta
      181 gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc agcagcggct
      241 ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac ggtcaggtca
      301 actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt gccattcagt
      361 tctggcagta ctttacctac agctttagca gcatcggtag aggacgggat gatgttctgg
      421 gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt tttctgagta
      481 gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt atcgttgata
      541 actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac gatgtcctgg
      601 ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc tttagacgga
      661 ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt ttcgtcagtc
      721 aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca tttcaggtta
      781 gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag atgaccgtct
      841 ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag catgtatgcc
      901 atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga acgtttctga
      961 gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac tttgatagtc
     1021 atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt acaaaaacct
     1081 tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg tgcctaagca
     1141 ttacgcgact gactcgcctc actcttcctt tgggcttgaa accacatggg gtcggcgccc
     1201 cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta ggtgcagcca
     1261 tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt tttgttagaa
     1321 tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta ataaaccttc
     1381 ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc agaatcatgg
     1441 gacagagccg ccatttacgg ggcgtttact gcataacaag cgtgacggcg tatatcactg
     1501 tttgatctgc gatgccccgc tgtttcattc cgaaaccaag tatgattccg gctgtggctg
     1561 gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag acttgtcaca
     1621 tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg ggcatgtctt
     1681 tcccgacggg ccgcaaccaa caggcgaacg ttattgtgtt aactctgctt ctttacgctt
     1741 cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag caacttattc
     1801 cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac gcctgaggta
     1861 taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ctgatggcgt tgcgttgacc
     1921 gaggaacaaa aagagaattg cctgcaactg gtgatgctgt ggcaagcccg ccataatacc
     1981 gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag caaacagcag
     2041 ttaaaagaag attttggtat ctcagcaaag cctattgcta tgtttaagtg aatgagttat
     2101 ctagtattta agcctgttat taagggaggg caaatatact tgttcggacg tcgtgtttta
     2161 gctgcaagta ggttaaatgc aacactgaca agtattatct ggcctacaat gtcattgagg
     2221 aaatgtaggc cagagatgaa ttcaactaat attacccctg tgtctcttcc cagtctgcca
     2281 gcgtatatag cgttgcccca gctgctgaca tctccataaa cgcgtgtgca ctgtcctggg
     2341 gctggatatt cacgccacga caaccatcgg taatcacgtt caccttataa cctaactgta
     2401 acgcgtccag cacggtaaac ttcacgcaat agtcagtagc caggcccata acgatcaatt
     2461 catcgatttc atgatcgcgt aaccagtcat cgagagaggt tttctgccga cggccgttat
     2521 caaaaaaggc actgtaactg tcaactaaag gattttcgcc tttatggaac accgctgcga
     2581 tcgctttttg gttcagtaac ggatgtaatt gtgcgccttc actgttctgc acacagtgat
     2641 ctggccagaa ggtttgtggc aaaccgtcga gttggcctgg cgtataaggc tctacaccgt
     2701 gctgactggc aaaactgccg tgattcgccg gatgccagtc ctggctggcg ataaccgctt
     2761 ctccgcgcga ctggcaccag tcaataaggc ggttagcaac ctccaccgta ctgtcacctt
     2821 ccggtacggc gagcgcgcca ccagcacaga aatcattttg taaatcgacc agtaacaggg
     2881 cgcgatgggg cattgcagtc tccttaatca tccggcgtca gttcgccgcg caggttttgg
     2941 ctcatggcct tgcgaatggt ttcagtatcc agttcctggc tcagcaggta atgcagtttg
     3001 gttagcgtgg cttctacagt catatctgca ccgccaataa cgccggcatg ggcgagggcg
     3061 ttaccggtgg cataaccacc catattcact ttaccggaca tacattgtgt caggttgacc
     3121 accacaatac cgcgatcgct ggcttcttgt aattcctgca ggaaggcttt gttttgtggc
     3181 gcattaccca cgccatagga gcgcagaatc aatgctttca ctggttggcg cagaaaattg
     3241 cgcacgacat cagcagaaat ccccggataa atcgtcacta cgccaattgg ttgtggcgtg
     3301 attggatgaa cgattaattc cccttcaccg tgcggggcgg gtggcgtatt caaacgacga
     3361 atatggatac cagcttccag taacggagga aggtttggag aggcaaacgc atcaaaacca
     3421 tcggcatggg ctttggtagt gcggttgccg cgatacaatc ggttattgaa aaagagcgtt
     3481 acttcgttga tcggataatt cgccgcaacg tacaacgcat tcagcagatt aatttgtccg
     3541 tcagagcgta actcagccag cgggatttgt gaccctgtca caatgaccgg tttaccgaga
     3601 ttctcgagca tgaacgacag cgcagaggcg gtatacgcca tcgtgtcggt accgtgtaga
     3661 atgacaaaac catcatagtc gtcatagtgc gctttaatat cttcagcaat atgctgccag
     3721 tcatccggcg tcatatcaga agaatccatc aacggcgtat attcatgaat ggtgaaatct
     3781 ggcatctccg ggcgatggaa ttccggcatc agcgccagtt ggcgttgtag atgacctgac
     3841 accggaatat aaccctgctc ggaacgctgc atcccgatgg tcccgcccgt gtaggcgacg
     3901 taaattgatt tcttttgcat gatattgata tgaatgatat cgaaagagca aaagtatata
     3961 cgtgaggcag gaggagcgca cctgatacta tgcggataag gaaatatggg gcgagacagc
     4021 gggaagagga ttcgtcgagg taaaaaataa tgtggccgac gatctgtcgg ccactcagta
     4081 caagacttaa cgcacgttgg cgcaggtcag gcaaaacgca taacggtttt gtgggtcgtt
     4141 aaacgcggcc agcttgtcgc tttcactttt aacggtagag gctaccgagg caagcggtgc
     4201 aggtaacatg gcctggaacg catctggcaa cattgcccgg acagaaccag acatgttgtc
     4261 catcactttg tcgaagaagg tcggttcatc aacgtagtat tccagatgcc actgtttcac
     4321 ttttgcgagc tctgctgctt tggcaaccgc atcatcgaaa tccccgagac tatcgaccag
     4381 cccgttagct tttgcatcct gaccggtcca gacgtggccc tgggcgattt tatcaatttg
     4441 ctccggcgtc gaatgacgcg catcagcaac cagcgtgata aagcgtttat agccattctc
     4501 aatgcttaat tgcatcatct gctgcgcttc cggcggcagt gccctggtga tagaaacatc
     4561 cgccagcggt gaagttgaga caccatcagt atgaacacca atcgaatcca gactattttc
     4621 tacggtggtg atcacgccga agataccgat agaaccggtc agggtgctgg ggttagccac
     4681 aatgtaatta gctggcgtgg aaatccagta accaccagat gccgccatgc cgcccatcga
     4741 tacaaccaca ggcttacccg ctgcccgggc tgctgccagt tcagcgcgaa tcacttcaga
     4801 cgcggtaacg ctgccgcctg ggctattaac acgcaggaca atcgctttca ctttcgggtc
     4861 aaggcgagcg tcgcggattt gtgccgcagt ggtatcaccg ccaacattcc cctgagtttc
     4921 ctcgccatcc ataattgcgc cattagcgaa gacgacaccg atgctgtcac cggtatctgc
     4981 cggcgttttc aatgcgtaat cgtaataact gatggcgcga taatttttat cagtcttact
     5041 ccagccgaac tctttggtca gagctttttc gatttcggca ctcgatgcca gtgcatcgac
     5101 cagcttgttt tccagtgcat atttcgcggt atcgccaccg gttttggtta aaccctcaag
     5161 caacccttgc gcgctaggga atacctgctg agcagggatc tgtcggttag cggcaacagt
     5221 attcagatag ttttgccaca gctcaccaat ccagcggctg tcagcttcgc gggctgccgg
     5281 tgacatatca tcacgaataa acggttcaac ggcagattta tacgtaccca cgcggaacac
     5341 atgggtggaa actttcagct tatccagcaa cgatttgtag tacagaccgt tggtagcaaa
     5401 accgtgcaga tcaaccacgc cttgcggtga cagccaaatt ttattggcga aactggcgag
     5461 ataatattgc ccctggctgt agttctcgcc aacggcataa accggtttcc cgctgtcacg
     5521 aaactctttc agggctttgc cgatgtactg catagacggc tggtcgccgc ctgcgaagtt
     5581 tttcagatcc atcacgatac cggtgatatt gtggtcgtcc ttcgcctggc gaatggtgtt
     5641 gacgatatca aacagtgagt tttcctgcag acgatcggaa ctggcaccaa gcagctggcg
     5701 gcttaattta ctaaaccgct gagaactgtc gggtttatcg acgatcacac cggaaatgtc
     5761 cagcagcagt gcgccacgac tggccgtttc tttcgaatca ccaccactga cctgcatcca
     5821 gatccccaca ccaaccagta cgaggaaaat aaagaacagg ttaagtacca tttcacggac
     5881 gaaattcagc agacgccacg tccatttaaa aaatccggca ataaatcgcc aaagggttcg
     5941 catgtattct cccaacttaa ggtcacacct gtcacaggcg caatagatgt ggttatcgta
     6001 atgacccaac cgacacttgt cagcaggaat tgcggactgt gctgtaacaa aatccactct
     6061 cgtgttaatt ttgtgaataa atatcacgac aggagttaat caaatggatg cactcgaact
     6121 attgatcaat cgccgtagcg cctcccgcct ggctgaaccc gcgccaacgg gtgaacaact
     6181 gcaaaacatc ctgcgtgcgg gtatgcgtgc gccggaccat aagtccatgc aaccgtggca
     6241 tttttttgtg attgaagggg aagggcgcga gcgtttcagc gccatactgg aacagggggc
     6301 gattgctgcc ggtagtgatg acaaagctat cgacaaagcc cgtaatgcgc cgttccgcgc
     6361 accgctcatc atcacggtgg tggcgaaatg cgaagaaaat cataaagtcc cgcgatggga
     6421 acaggaaatg tctgccggat gcgcggtcat ggcgatgcaa atggcagccg ttgcccaggg
     6481 atttggcggc atctggcgca gtggcgcatt aactgaaagt ccggtagtgc gtgaagcatt
     6541 cggttgccgt gagcaggata aaattgtcgg ttttctctac ctcggcacgc cgcagttgaa
     6601 agcatctacg tcgattaacg tcccggaccc gacgccgttt gtaacttatt tctgataatt
     6661 gtcgcgaaac tgtctggatt gtgagcaaag gcgcaggaat tcagacactc tcacttatca
     6721 cttcacggaa tgagggttac catacccgga ttgcattgac aggagatgtc catgagcgag
     6781 aactcgattc gtttgaccca atacagccac ggagctggtt gcggctgtaa aatttcccca
     6841 aaagtgttgg aaaccatcct gcacagtgag caggcgaagt ttgttgatcc gaatttgctt
     6901 gtgggtaatg agacccgcga cgatgcggcg gtgtacgatc tgggcaatgg caccagcgtt
     6961 atcagtacca ccgacttctt tatgccgatc gttgataatc ctttcgattt tggccgcatt
     7021 gcggcgacta acgccatcag cgatatcttc gcgatggggg gcaaaccgat tatggcgatt
     7081 gcgatcctcg gctggccgat taacaaactt tccccggaaa ttgcccgcga agtaaccgaa
     7141 ggtggacgct atgcatgccg tcaggcgggt attgcgctgg ctggcggtca ctccatcgat
     7201 gcgccggagc cgatttttgg tctggcggta acggggatcg taccgaccga gcgggtgaag
     7261 aaaaacagca ccgcacaagc cggatgcaaa ctgttcctga cgaaaccgct ggggatcggc
     7321 gttcttacca cggctgagaa aaaatcactg ctgaaaccag aacatcaggg actagcgacg
     7381 gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg ccaacatcga aggcgtaaaa
     7441 gcgatgaccg acgttacggg ctttggtctg ctgggccatt tgagcgaaat gtgtcagggc
     7501 gcaggtgtgc aggcgtgcgt tgactatgac gcgatcccaa aattgccggg ggttgaagag
     7561 tacattaagt tgggcgcagt gcctggcggc accgaacgta actttgccag ctacggtcat
     7621 ctgatgggtg aaatgccgcg tgaagtgcgc gatttgctgt gcgatccgca aacttctggc
     7681 ggtttgctgc tggcggtcat gccggaagca gaaaatgagg tcaaagctac agccgccgag
     7741 tttggcattg aactgacggc aattggcgaa ctggtgccag cgcgcggcgg tcgtgccatg
     7801 gttgagattc gttaattcaa tgcggttgtt tattgccgaa aaaccgagtc tggcgcgcgc
     7861 cattgctgat gtcctgccca aaccgcaccg gaaaggcgat ggctttatcg agtgcggtaa
     7921 tggtcaggtg gtgacctggt gtatcggtca cctgcttgag caggcgcagc cagacgccta
     7981 cgacagccgc tatgcgcgct ggaatcttgc ggatttgccg attgtcccgg aaaagtggca
     8041 attacagccc cgaccctccg tgaccaaaca acttaacgtc atcaaacgct tcctgcatga
     8101 agccagcgaa atcgttcacg ccggggaccc ggatcgtgaa gggcaactgc tggtggatga
     8161 agtgctggac tatctgcaac tggcaccgga aaagcgccag caggtgcagc gttgcttgat
     8221 aaacgacctg aacccgcagg cggttgagcg ggcgatcgac cgtcttcggt ccaacagtga
     8281 gtttgtgcca ttgtgcgttt ctgcgctggc gcgagcgcgt gccgactggc tgtacggcat
     8341 caatatgacc cgtgcgtata ccattctcgg tcgcaatgcc ggttatcagg gcgtactttc
     8401 cgtgggacgc gtgcagacac ccgtgctcgg gctggtggtg cgccgcgatg aagagattga
     8461 aaacttcgtg gcgaaagact tctttgaagt caaagcacat atcgtgacac ctgccgatga
     8521 gcggtttacc gctatctggc aaccgagcga agcgtgtgaa ccgtaccagg atgaagaagg
     8581 gcgcttgtta catcgtccac tggcggagca tgtggttaac cgcattagtg gtcaaccagc
     8641 aattgtcacc agctataacg ataaacggga atcagaatcc gcgccgctgc ctttttcgct
     8701 ttcggcgttg cagattgaag cggcaaaacg ctttggtctg agcgcgcaga acgtgcttga
     8761 tatctgccag aagctgtacg aaacgcacaa gctaatcact tatccgcgtt ccgattgtcg
     8821 ctatttgcca gaagaacatt ttgccggacg ccacgcggtg atgaatgcca tcagcgttca
     8881 tgcaccagat ctgttgccgc aaccagtggt agatccagat atacgcaacc gctgttggga
     8941 tgacaaaaag gtcgatgcac accacgccat catcccgacc gcacggagtt ctgcgatcaa
     9001 cctgacggag aacgaagcga aggtctataa cctgattgcc cgtcagtact tgatgcagtt
     9061 ctgcccggat gcggtgttcc gcaagtgtgt tatcgaactg gacattgcca aaggcaaatt
     9121 tgtcgctaaa gcgcgttttc ttgctgaagc aggctggcgc acgctgttag gcagcaaaga
     9181 gcgcgatgaa gaaaacgacg gtacgccatt gcctgtggtg gcgaaaggcg atgagttgct
     9241 gtgtgaaaaa ggtgaagtgg tagagcggca aacccagccg ccgcgccatt ttaccgatgc
     9301 aacactgctt tcggcgatga ccgggatcgc gcgctttgtg caggacaaag atctgaaaaa
     9361 gatccttcgt gcgaccgatg gtctggggac agaagcaacc cgtgccggga ttattgaact
     9421 gctgttcaag cgtggttttc tgaccaaaaa agggcgctat atccactcta ccgacgccgg
     9481 aaaagcgtta ttccattcgc tgccagaaat ggcgacgcga ccggacatga ccgcgcactg
     9541 ggaatcggtg ctgacgcaaa tcagcgaaaa gcagtgtcgc tatcaggact ttatgcagcc
     9601 gctggtgggg acgctatatc agcttattga tcaagccaaa cgtacgccgg tgcggcagtt
     9661 tcgcggcatt gtggctccgg gcagtggtgg cagtgctgat aagaaaaagg ctgcaccgcg
     9721 taaacgtagt gcgaaaaaaa gtccgccagc agatgaagtc ggaagcgggg cgatagcgta
     9781 agcgagtgaa tctttcgtgc tattcgagtc atattctgaa atatccagcg aatcaagaaa
     9841 attcgttgga tatttttttg catggataaa attatcgcct ctaaagtatg taataacagg
     9901 gaatgtgatg aaaaaagttt tattacaaaa ccatcctggg agcgagaagt attcttttaa
     9961 tggctgggaa atatttaata gtaattttga acggatgatt aaagaaaata aggccatgct
    10021 gctttgtaag tgggggtttt atttaacatg tgttgtcgct gtaatgtttg tgttcgcagc
    10081 gataacatcc aacggtttga atgaaagagg cctgattacc gcgggatgct cttttcttta
    10141 tctattaatt atgatg
//
LOCUS       DAJTOR010000151        10126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTOR010000151 DAJTOR010000000
VERSION     DAJTOR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.151"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..226
                     /locus_tag="OO249_003290"
     CDS             <1..226
                     /locus_tag="OO249_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637474.1"
                     /translation="EEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEK
                     TYTAELSSIAGTPIEAGPFDTVVLFKINYN"
     gene            378..1424
                     /locus_tag="OO249_003291"
     CDS             378..1424
                     /locus_tag="OO249_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5637475.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTCM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPDGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(1421..2212)
                     /gene="nei"
                     /locus_tag="OO249_003292"
     CDS             complement(1421..2212)
                     /gene="nei"
                     /locus_tag="OO249_003292"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5637476.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2248..2982)
                     /gene="pxpA"
                     /locus_tag="OO249_003293"
     CDS             complement(2248..2982)
                     /gene="pxpA"
                     /locus_tag="OO249_003293"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5637477.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(2972..3904)
                     /gene="pxpC"
                     /locus_tag="OO249_003294"
     CDS             complement(2972..3904)
                     /gene="pxpC"
                     /locus_tag="OO249_003294"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR5637478.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3898..4554)
                     /gene="pxpB"
                     /locus_tag="OO249_003295"
     CDS             complement(3898..4554)
                     /gene="pxpB"
                     /locus_tag="OO249_003295"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5637479.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIELLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4577..5320)
                     /gene="ybgI"
                     /locus_tag="OO249_003296"
     CDS             complement(4577..5320)
                     /gene="ybgI"
                     /locus_tag="OO249_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5637480.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5591..7072
                     /gene="dtpD"
                     /locus_tag="OO249_003297"
     CDS             5591..7072
                     /gene="dtpD"
                     /locus_tag="OO249_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5637481.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(7114..8532)
                     /gene="phrB"
                     /locus_tag="OO249_003298"
     CDS             complement(7114..8532)
                     /gene="phrB"
                     /locus_tag="OO249_003298"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5637482.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACSNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8529..9038)
                     /locus_tag="OO249_003299"
     CDS             complement(8529..9038)
                     /locus_tag="OO249_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5637483.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(9323..9697)
                     /locus_tag="OO249_003300"
     CDS             complement(9323..9697)
                     /locus_tag="OO249_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637484.1"
                     /translation="MFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIKYQKY
                     ENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLIFMADFGESRYFKPAKI
                     AMNKNNLIIRTSDVEIAFKITK"
     gene            complement(9682..>10126)
                     /locus_tag="OO249_003301"
     CDS             complement(9682..>10126)
                     /locus_tag="OO249_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5637485.1"
                     /translation="IDKPAPVFNRDLNSDPYYLAVNNCYSYALNRYGNPGSRIFGGGG
                     LQPGELSGKEFSKLTCGSIFEASKNDGAKDLDNGSCPSGYHKAQLFIRPHNFIGMGGD
                     YHWYRQDANGEWSDKQGVGAIRFRGKDPFPPIDYPEKCGTIFLPN"
BASE COUNT         2358 a         2749 c         2434 g         2585 t
ORIGIN      
        1 tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa
       61 cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt
      121 ggtctacgaa aaaacctata cagctgaact ttcgtccatt gcaggtaccc ccatcgaggc
      181 tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc
      241 ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcctct tttccttgat
      301 tactgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa
      361 taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc
      421 tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga
      481 tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc
      541 tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca
      601 ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca
      661 gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct
      721 ggggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag
      781 atattcgcct ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg
      841 ttctggtcac ctgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct
      901 ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta
      961 cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg
     1021 cagtgctcca gtctggtcag ctcatcacca tcgaactacc ggaatggctg ctggcgatgg
     1081 cgtatatggc aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg
     1141 cactacgccc actaccgcaa atcctgctgt cgatttttgc tctgctggct atttgtgcag
     1201 gtatggcgtg gggactgacc cggtttatgc atattgattt tatgactgcc tacctcgcca
     1261 ccagtcccga cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg
     1321 cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg gggccagcca
     1381 ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc ctagtgctgg cagcctggac
     1441 accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca cagcgttcgc
     1501 acagttcgcc atcacgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct
     1561 catccacctg cccccgcgta gcgtaagaaa accgaggaat ctccagtaac gcgtgtgcga
     1621 gtgcatccag ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct
     1681 gccagaggat ctccacccgc aaataattgc caagcccagc cagaaacgcc tgatcgagca
     1741 gtaatcctgc aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc tttaccacct
     1801 ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg
     1861 tcagttgttc cggcctcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt
     1921 cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa
     1981 ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaagtcg ttggaaaaat
     2041 gagttaacaa cgccttacca cgcgttccca catgggtaac gtgttgaccg ataagttgtg
     2101 attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga
     2161 tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat
     2221 ccttttttca ttctttatta atcggggtta tgctgcgaca acaatctcct tttcggcaaa
     2281 tgtagagcgt agtcggcggg cgaacgccag tgcgtgctcg ccgtcgccat gcaggcagac
     2341 tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac
     2401 catctctagc gtttgcgcca gcgcctgttc ttcgttttca atcaacgcgc ctggctgact
     2461 tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt
     2521 tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaac
     2581 caatgctgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt
     2641 cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc
     2701 acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg
     2761 cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc
     2821 aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gggcatcgcc
     2881 tgcatgaaat ccacaagcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct
     2941 ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgtagc cgccacgcta
     3001 attgttcgaa ataacgttgt tgatcttgcc gcgctttcag tgcctcttcc agtgaacact
     3061 ggacaaaatg aatcggctga ccgagcggaa tttgcgccag atggtacata tcagcctcaa
     3121 tgatacaggc aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg
     3181 gctgcccgtt atgcggcacc tgcaccacac ccggtaacaa accgtgagat aacagttcgc
     3241 gatcggtggt gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg
     3301 ggctaagctg ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat
     3361 attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg
     3421 cttccataaa atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc ttcagtaaac
     3481 ggccttccag cccgccaatc cccacgttga gatcggtgct gcatgacccc attaccggcg
     3541 gaacatcaat accacccgcg accgccagat aactgcgcat gccgtgctgc gggcgtttaa
     3601 gcgttaaacg ctggcctgct ttcatcggca agcgccagcc ggtccagacg gcgttatcat
     3661 ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt tcgaactcaa
     3721 ccgttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagca
     3781 ggttagcaat gcgtaacgca ggcatatcca gtgcgccgca gtggctgata cccgactggc
     3841 gaaaaccgtg acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca
     3901 gcatactccc tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg
     3961 ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc
     4021 cggcgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg
     4081 aacgagcaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag
     4141 atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa
     4201 ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc
     4261 gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc
     4321 gctctcctcc caccagcgtt gcaaaagctc tatggcatcc agcgccagcg actcaggatt
     4381 acgcaaaatc accgtgatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc
     4441 caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc
     4501 cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga
     4561 tccctctgat tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg
     4621 tattttcatt cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac
     4681 ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt
     4741 cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc
     4801 cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg
     4861 gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg
     4921 tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg
     4981 ccgccagctg tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat
     5041 acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga
     5101 ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac
     5161 gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg
     5221 tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca
     5281 gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct
     5341 gatagctaca ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac
     5401 atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc
     5461 taatgtatat ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt
     5521 tttaacttta aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg
     5581 tggaattatt atgaataaac acgcatcaca gccgcgcgct atttactatg tcgttgcgct
     5641 gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac
     5701 caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct
     5761 ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat
     5821 ggcggtgatg ctgggggcgt tattgatggc gatcggtcat gtggtgttgg gtgccagtga
     5881 gatccatccg tcattcctct atctgtccct ggcgataatt gtttgtggct atggtctgtt
     5941 taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga
     6001 tggcggtttc tcgctgatgt atgcagcggg taacgtgggg tctattatcg cacctattgc
     6061 ctgtggctac gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg
     6121 tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg
     6181 cgttaataaa aaagtactgc gtgcgacaaa ctttctcctg cctaactggg gatggctgct
     6241 ggttctgctg gtggcaacgc ctgcgctgat taccgtattg ttctggaaag agtggtcggt
     6301 atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa
     6361 agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag
     6421 tatgttgttc tgggccttcg cacaacaggg cggcagctcg attagccttt atatcgaccg
     6481 cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa
     6541 tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc
     6601 aggtaatcgt accgtgcgca tctggggaaa atttgctctt ggtctcggcc tgatgagcgc
     6661 cgggttctgc attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct
     6721 gccactgatg gtattaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt
     6781 tgccatgtcg caaattacgc gcattgaaat ccccggtgtg accggcgtat taaccggcat
     6841 ttatatgctg ctttccggcg cgattgcgaa ctatctcgcg ggcgtgattg ccgatcagac
     6901 atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga
     6961 agtatttgat caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg
     7021 gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat aataggccag
     7081 caccatacct gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta
     7141 cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag
     7201 catcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc
     7261 gcgcagctcc ggtaaccatc gacgaataaa ctcgccctca cggtcaaatt tctcgccctg
     7321 ggttgtcggg ttgaaaatac gaaaatacgg cgctgcatcg gttccggttg aagcggccca
     7381 ctgccagcca ccgttattgg ctgccaaatc accatcaatc agctgcgaca tgaaatatcg
     7441 ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg taatcatccg
     7501 tagccggtta tgcatccagc cagtgctgtt aagctgacgc atggcagcat cgacaatcgg
     7561 gtatcccgtt ttgccttcct gccaggcctg taaatgtgcg ggattgctct gccactgcac
     7621 acgatccgtc caggcaataa acggacaatg tttacacaac gaggggtaat acgtcatcag
     7681 atgacggtag aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc
     7741 cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc
     7801 cgttgccagg ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg
     7861 ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc
     7921 ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg
     7981 cgagggctct atcgatccgc tactacgaac ttttggcgca gcgacgcact ccggcatccc
     8041 ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg
     8101 attaccggtc atcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac
     8161 cacgttacgc agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg
     8221 atagttataa aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac
     8281 actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc
     8341 tatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg
     8401 cgtcgcccac tggcgtggtg tagcgatata caacgccagc acgcgtgcag acgaattgct
     8461 gcaggcggca gccagtgcga gattatcgtg caaacgtaaa tcctggcgaa accagaccag
     8521 atgggtagtc ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa
     8581 taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc
     8641 agcggcgcaa gaagtggttg ctcgccacgg cgataactgt cgataagcgc agccagctgc
     8701 tggcgttctg tggaatcaat atgcgggcga aaataaccct gaacgtgcat caaaacattg
     8761 gtgtgatcgc gcgggttggc ggggtgagaa agcaaaacaa ctacgcgttg gcggtactgg
     8821 ttgtaatagt catccagatt ttgccactgg tgaatatcag ccacgaacgg gccaagtttg
     8881 cggtactccg gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga
     8941 agcgccccgc gcgttaagcc atgttctttc agtcggtgta actcatgaag ggcaaagata
     9001 cggattaggg tgctgtcatc aaatcgttgt agattcatta ctgctcctga acggtataaa
     9061 ggagtaagtg tagacgatgg taaatgaatt ttatggggca gagtttttgc ggggaagtat
     9121 tttgttggta aaaatattat caacttcatc tgagcgagaa ttattctact atttcccgag
     9181 ctgcattcat tttttgtatt ttttattttt agaaaataac agttctcaaa gcttaattac
     9241 ctcactatta attgtatttc aaactaacaa tcgcactcca ttttctctct ttaaaaaaca
     9301 taaacactgt taaaacaaat atttatttcg ttatcttaaa tgctatttct acatcactcg
     9361 tccttataat caaattattc ttattcatag ctatttttgc aggtttaaaa tagcggcttt
     9421 caccaaagtc agccatgaaa attagactac aagttctccc agcctttgca catgagaaaa
     9481 accacccatc aactctattt attcctgaac ctttaacacc ataataaatc agaatttctg
     9541 ggtattgttc atccttcacg ttttcatatt tttggtattt aattgaaaac caagagtcta
     9601 tactgccaac atcttcaaaa aatattggag ctgatgtttc cgcatgaaca tatgcagaaa
     9661 atgctaacaa aatggcaata cttagtttgg taaaaatatc gttccacact tttcaggata
     9721 atctattggg gggaagggat cttttcctct aaatctaatt gctcccactc cttgtttgtc
     9781 agaccattcg ccatttgcat cctgacgata ccagtgataa tcccctccca tgcctataaa
     9841 attgtgagga cgaataaaca attgagcttt atggtaacca gatggacaac tcccattatc
     9901 taaatccttg gcaccatcat tttttgacgc ttcaaaaata ctaccgcaag tgagtttaga
     9961 aaactctttt ccagataatt ctccaggttg taatcctccg ccgccaaaaa tcctactccc
    10021 agggtttcca tacctattta gagcatatga ataacaatta tttacggcaa gataatacgg
    10081 atctgaattt aggtctcgat tgaatactgg agcaggttta tcaatt
//
LOCUS       DAJTOR010000152        10105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTOR010000152 DAJTOR010000000
VERSION     DAJTOR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.152"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            203..355
                     /gene="hokA"
                     /locus_tag="OO249_003302"
     CDS             203..355
                     /gene="hokA"
                     /locus_tag="OO249_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5637486.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(543..755)
                     /gene="cspA"
                     /locus_tag="OO249_003303"
     CDS             complement(543..755)
                     /gene="cspA"
                     /locus_tag="OO249_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5637487.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1036..1326)
                     /locus_tag="OO249_003304"
     CDS             complement(1036..1326)
                     /locus_tag="OO249_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR5637488.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1759..2469
                     /locus_tag="OO249_003305"
     CDS             1759..2469
                     /locus_tag="OO249_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5637489.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2519..3493)
                     /gene="ghrB"
                     /locus_tag="OO249_003306"
     CDS             complement(2519..3493)
                     /gene="ghrB"
                     /locus_tag="OO249_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5637490.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKPSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3597..4256)
                     /locus_tag="OO249_003307"
     CDS             complement(3597..4256)
                     /locus_tag="OO249_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5637491.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4409..6742
                     /gene="bisC"
                     /locus_tag="OO249_003308"
     CDS             4409..6742
                     /gene="bisC"
                     /locus_tag="OO249_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5637492.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVHVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVHAAKIRELAALFHQNTTMLMAGWGMQRQQLGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRCSAVLSSMQGSLPGGSDAVDK
                     IPVARIVEALENPGCAYQHNGMNRLFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDALARGIQD
                     GDTVRLWNARGQILAGVVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6711..7151)
                     /locus_tag="OO249_003309"
     CDS             complement(6711..7151)
                     /locus_tag="OO249_003309"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5637493.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7148..7711)
                     /gene="tag"
                     /locus_tag="OO249_003310"
     CDS             complement(7148..7711)
                     /gene="tag"
                     /locus_tag="OO249_003310"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5637494.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7869..8567
                     /locus_tag="OO249_003311"
     CDS             7869..8567
                     /locus_tag="OO249_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5637495.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8795..9997
                     /locus_tag="OO249_003312"
     CDS             8795..9997
                     /locus_tag="OO249_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5637496.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2423 a         2435 c         2591 g         2656 t
ORIGIN      
        1 ttttaggatt atatcaagga gaagaaacaa acttattaag ctagaatagc tacgggtgct
       61 tgagactgtt tgtctcaggt attcaccgaa aggcagacag agaaaagccc cacctgacta
      121 taaatcaaag tgaggctacc ctatgcctga acaccataag gttagcctct tactcgttgg
      181 aaatcaacac agggggctgg gaatgccgca aaaatacgga ttactttcat taatagtgat
      241 ttgtttcacg cttttatttt tcacctggat ggtaagagat tcactgtgtg aattgcatat
      301 taaacagggg cgttatgagc tggcggcatt tttagcctgc aatttaaaag agtaagagtc
      361 ttcggcggga aattattccc gcctttctta cggcgttgcg cattctcatt gcacccacat
      421 ttattcttca caaaaataat aatagatttt actacgcgat cgattattta tttcctgaaa
      481 aaaaataaaa aaaatccccg ccaaatggca gggatcttag attctgtgct tttaagcaga
      541 gattacaggc tggttacgtt accagctgcc gggcctttag cgccgctttc gatggtgaag
      601 gacactttct gaccttcgtc cagagatttg taaccatcgt tctggatagc agagaagtgt
      661 acgaacacat ctttagagcc atcgtcagga gtgatgaagc cgaagccttt gtcagcgttg
      721 aaccatttta cgataccagt cattttaccg gacatagtgt attaccttta ataattaagt
      781 gtgcctttcg gcgatatggc gtgctttaca gattttgaag cgttaaagga atgtgcacta
      841 cgaggggtat caacgataac tcttgaaggg acttgcctta ctacactgct ttaatggtct
      901 gtacgtcaaa ccgttgatgt gcattaagcc acgcattggc gggtgatgca acaattattt
      961 ttcatattta tgattaatcg gttggaaaac ggtcctgtca tcaggaccgt aaacagcaat
     1021 aaagtggata aaagtctatt ccatcaactg cttacttaat gccgggttgg cttgaatcaa
     1081 acgcatcaat tttagttcgg cacttgaagg cttcacgcgt ctggattccc attcctttac
     1141 catggcgact gatatgccca aaacccgggc gaaatcatcg atttttaacc ctgtcccttt
     1201 tcgtaactgc tcaatttcgg tacaggacgt tgttctgtgc gtcagggtaa ttttctgcgt
     1261 ttcatcttta aaaacaatct gttccaggct gctcaacagc tcatgcattg gatctttata
     1321 ttccattgaa aactcctcaa atcacactgc gggatcgtga attgcatcga agctcattaa
     1381 gaatagtcgg gaaaagcaga cacagcgggg tgaagtcagg gcatgctctg gctatgattc
     1441 gattcagcca gtcatttagc gtgacttata gccacctaac gatctgataa atgccgatat
     1501 atctcttacc actcctgttt tgtaaagatt tcgaaaatct aaagttctgc gaaggcgtaa
     1561 atctcgggct acaaaaaagc aacggcaata tcatttttta acaatgaaac aacaaattaa
     1621 ccttgtttca ttttctccat gattttacca ggaagattac gtagaaaggg tatcctgcaa
     1681 gcctgtcctg gactatcctt gtcacgtcag acacgcgtgt gtcgttgtgc gctttttttg
     1741 ggtgaaagga gtaagaaaat ggcgacagga aagtcctgct ctcgctggtt tgcgcctctt
     1801 gcggcgttat taatggtagt tagcctgagt gggtgttttg ataaagaagg cgatcagcgt
     1861 aaagcgttta tcgacttcct gcagaataca gtgatgcgta gcggtgaacg tctaccaacc
     1921 ctgactgccg atcagaaaaa acagtttggt ccttttgtct ctgattacgc gattctgtat
     1981 ggttattctc agcaggtgaa tcaggcgatg gattccggtc tgcgcccggt cgtagacagc
     2041 gttaacgcaa ttcgcgtacc gcaggattac gttacgcaaa gcggtccgct gcgtgaaatg
     2101 aacggttcgc tgggtgtgct ggcgcaacaa ctgcaaaatg cgaagttgca ggccgatgct
     2161 gcgcactctg cgctaaaaca gaccgatgat ttgaaaccgg tctttgatca ggcattcacc
     2221 aaagtggtaa cgacgccagc tgatgcattg caaccgttga ttccggttgc acaaactttc
     2281 acgcaacaac tggtgatggt tggagactac attgctcaac aaggtactca ggtgagcttt
     2341 gtagcaaatg gtatccagtt cccgacttcg cagcaggcaa gtgaatataa caaactgatt
     2401 gcgccgttac cggcacaaca tcaggcgttt aatcaggcct ggactacagc agttactgca
     2461 acccaataaa gagtaaaagc ccgagcgatt atcgggcttt acttcacgca gtcgcggctt
     2521 agtccgcgac gtgcggattc acacagttct tctcaacctt tccttgtaac gcatcaatca
     2581 aattatccac ggcacaggcg gccatgccat aacgcgtctc atgggtggca gatccaatat
     2641 gcggtactgc gacgacgttg gccattgaga gcaacggcga atctacggac agtggctctt
     2701 gttcgaagac atccagcccg gcggcgtgaa tttcgccttt ctgcaatgct gcgatcagtg
     2761 cattttcgtc aaccaccggg ccacgtccgg cattaatgaa aatggcggag ggtttcattt
     2821 tggcgaattg ttctgcgcca aacagatgat gcgtctcatc agttaacggc aggatcaggc
     2881 aaacgaaatc tgactcttgt aacagagtat ccaaatcgca gtagcgggcg ttgaagcgtt
     2941 cttctgcttc tttatggtgg cggcgcgcgt tatagaggat gggcatgttg aagccaaagt
     3001 gcgcacgttg tgccagcgcc atgccgatcc gtcccatccc gacaatgccc agtgttttat
     3061 ggtgaacgtc agtgccgtac cagtccgggc ctatgctcgc ggtccattcg cctgctttta
     3121 cccgttctgc tacctccaca acccgacgag cggtagacaa caccagcgcc atcagcgtat
     3181 cggcgacggt ttctgttaat acggttggcg tgtgcatcag cagaattttt cgggcggtaa
     3241 gcgcatcgac atcaaaattg tcatagccga cggagatcgt tgatgtggca cgcagtttcg
     3301 gcattttttc cagcaatgcg gcatctacat tctcgtttga acccagtaaa ccttcagctt
     3361 cggcaaaaat tgctgcattt tgttcgacgg tttgtgggct gaggtttgcc acctggtgaa
     3421 cggtgaaatg ctcttgcagg cgttgcagta aatcatcagg taaggctttg tagaggataa
     3481 cggacggctt catgcttctc tccattcact gataaggcgc tcaccttagc ctgaaatatg
     3541 atgaaggaaa actgaagaat gccaggtgat ttctgcatca cctggcatga aagggattac
     3601 agcgggctta aggtaatttc tacacggcgg ttttgcgcct taccttctgc ggtgctgttg
     3661 ctggcgattg ggttagccgg gccaaggccc tgagtacgga tgcggctggc gtccacgccc
     3721 tgggtgatca acgcgctggc aacggaatcc gcacgctgct gggagagacg catgttcagg
     3781 tcgtgacccc ccgtgctgtc ggtataacca atcacgttaa ccgccgtttt cggatactct
     3841 ttcagcacca ttgccacgcc ggtcagggtg ttagcgcctg ccggtttcag ggtcgcgctg
     3901 ctgctgtcga aggtcacatt gttcggcata ttgaggataa tgttatcccc gctgcgggtt
     3961 acgctaacac cagtgccgcg cattttgtca cgcagcttcg cttcctgcac atccatgtaa
     4021 taacccacgc cgccgcccag agctgcgcct gctgctgcgc caatcagcgc gcctttaccg
     4081 cgatctttct tcgaagaaga gagcgcacca atacccgcgc ccacgagaga gtccagacct
     4141 gcgccgatag cagatttgcc tgcttcgcgt tcgccggtgt aagggttagt tgtgcagcca
     4201 gataccgcca gagcgccact cactacggcg gcaataagat aaacacgttt cttcattgtt
     4261 aatccttaat aaccttttta ttctttgcca cgggttccgt ggcgggagat tatgccgcgt
     4321 gaacatgaag attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa
     4381 tacatctaac ctttcaggag tcttcggttt ggccaactca tcctcacgat attccgttct
     4441 gactgccgcc cactgggggc ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc
     4501 gcgtggcgcg ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt
     4561 tcacagcaat acgcgggtac gatttccaat ggtgcgcaaa ggctttctcg cgtcaccgga
     4621 aaacccgcaa ggcattcgtg ggcaggatga atttgttcac gtgagttggg atgaggcgct
     4681 ggatcttatt caccaacaac ataaacgcat tcgtgaggct tatggtccgg catcgatttt
     4741 tgctggttcc tacggctggc gttcaaacgg cgtgctgcat aaggcctcga cattattaca
     4801 acgctatatg gcgctggcag gcggttatac cgggcatctg ggggattatt cgaccggtgc
     4861 ggcacaggcg atcatgccgt atgtcgtggg tggcagcgaa gtttatcaac agcaaaccag
     4921 ttggccgctg gtgctggaac atagcgatgt cgtggtgttg tggagtgcta acccactcaa
     4981 tacgctgaaa attgcgtgga atgcatctga tgagcagggg ctttcttact tttccgcact
     5041 gcgtgacagc gggaaaaagc tgatctgtat tgatccaatg cgatcggaaa ccgtcgattt
     5101 ctttggcgat aaaatggagt gggtggcacc gcacatgggc accgatgttg cgctgatgct
     5161 ggggatcgcc catacgctgg tggaaaatgg ttggcacgac gaagcgtttc tggcgcgttg
     5221 caccacaggt tatgccgtct tcgcctctta tttgctgggc gagagtgacg gaatagccaa
     5281 aaccgccgaa tgggcggcag ggatttgtgg ggttcacgca gcgaaaattc gcgagttggc
     5341 ggctcttttc caccaaaaca cgaccatgct gatggctggc tggggaatgc agcgccaaca
     5401 gcttggtgag caaaaacact ggatgatcgt cacgctggcg gcaatgttgg ggcaaatcgg
     5461 cacacccggc ggcggttttg gtctttctta tcattttgcc aatggtggta atcccacgcg
     5521 gtgttctgcg gtgctctctt ccatgcaggg tagcttgccg ggtggcagcg atgcggtaga
     5581 taaaatccct gttgcccgta ttgttgaagc actggaaaac cctggctgcg cataccaaca
     5641 caatggcatg aatcgcctct tcccggatat tcgctttatc tggtgggcgg gtggcgccaa
     5701 cttcacccat catcaggaca ctaatcgcct gatacgagct tggcaaaaac cggagctggt
     5761 agttatctcc gaatgcttct ggacggcggc ggcaaaacac gcggatatcg ttctgcctgc
     5821 gactacctct tttgagcgta atgatctcac catgaccggc gattacagta atcagcatct
     5881 ggtgccgatg aagcaagtgg tgccgccacg ctatgaagcg cgtaatgatt ttgatgtttt
     5941 tgccgagtta agtgaacgct gggagaaggg cggttatgca cgttttacgg aaggaaaaag
     6001 tgagttgcaa tggctggaaa cgttttataa cgttgcccga cagcgcgggg caagccagca
     6061 ggttgaattg ccgccatttg ctgagttctg gcaagccaat cagttaattg agatgccgga
     6121 aaacccggac agcgagcggt ttattcgctt cgctgatttt tgccgcgatc cgcaggcgca
     6181 tccattaaaa accgccagcg gtaagattga aatcttctcg cagcgtattg ccgattacgc
     6241 ttatccggat tgcccggggc atccaatgtg gctggagccg gacgaatggc agggcaatgc
     6301 cgaaccggaa cagttacagg tactttctgc tcatccggcg catcgtctgc acagccagct
     6361 gaattacagt tctctgcgcg aattgtatgc ggtggcaaat cgtgagcctg tcaccattca
     6421 tcctgacgat gccctggcgc gcggcattca agatggcgat actgttcggc tgtggaacgc
     6481 acgcgggcaa attcttgccg gagtggtcat tagcgaggga attaaacctg gcgtgatttg
     6541 tattcacgaa ggggcatggc cggatttgga tttaaccgct gacggtattt gtaaaaacgg
     6601 cgcagtcaac gtgctgacca aagatctccc cagctcgcgg ctggggaatg gctgtgcggg
     6661 taatacggcg ctggcatggc tggaaaaata caacggtccg gaactgacac ttacagcgtt
     6721 tgaaccaccg gccagctcat aatccaggtg ggtagttggg tttcatcctg ccatgcgcaa
     6781 tcgacaatgt gaaaaccctg cgcctggtaa aaatttatcg ccggttgatt tttttgataa
     6841 acctccagca tcaggtgggg atggcgctgc tgcacatatt gcatcagcgc cttaccaata
     6901 ccgcgcctga cggccttcgg tgcgacaaac atcgctgcca gaaatcggcc ttccataatg
     6961 ctgacaaaac cgagaagctt actgtcttct tcccagaccc agttttgcgc attggcaaga
     7021 tacgcatccc gcaccagcgg aatgcaatca cgccagtaat tcgcttttat aaagggatgt
     7081 ccccaggttg tactttccag ccacagttcg aggatcgcgg ggagttctga acgttgcgct
     7141 tcccgaatca tggtttattt cccggatagc aacagcagcc aaccacatga tcattcacca
     7201 gcccacatgc ctgcataaag gagtaacaga ttgtggtacc gacaaactta aaaccacgtt
     7261 ttttcagtgc tttagatagg gcgtcggagg cgggcgtaga tgtggggatt tcgctcaacg
     7321 ttgtggcttg tgtcacctgt ggctgatgat ttacaaacga ccagacaaag tcggcaaacg
     7381 gttcgccgtt ctgttccatt tgcaggtacg cccgcgcatt accaataatt gcctgaattt
     7441 tcccgcgatg gcggataatc ccggcgtcct gtaccagtct ttcgacatcc tcttcctgca
     7501 ttgctgcgac cttcaccgga tcgaactgat gaaagcaggc gcgatagttt tcgcgttttt
     7561 tgaggacggt gatccacgat aacccagcct gctgcccttc aaggcagatc atttcgaaca
     7621 gttttttact gtcagtttca ggcacgcccc actcattatc atggtaggca atataaagcg
     7681 ggtcctgact cacccagccg caacgttcca tactttccct cgctatgtac ccgatcgtaa
     7741 aaatttcagc cgcttttctt gacgcatcgg tgaaaaagtc aatatttaat acagggctac
     7801 tatgcccgat atccttcata caatgacaat aatattgccg aattcggctc ttccctggag
     7861 tcgtgttaat gatcataaaa aaaagcggtg gtcgctggca gctaagcctg ctggcgagcg
     7921 tggtaatcag cgcctttttt ctcaacacag cttacgcctg gcaacaagaa tatatcgttg
     7981 atacgcaacc cggacattcc acagagcgtt acacctggga tagtgatcat caacctgatt
     8041 acaacgatat tttgtcgcaa cgtattcaaa gtagccaaag ggcgctggga ctggaagtca
     8101 atctggcgga agaaactcct gtggatgtga ccagcagtat gagtatgggc tggaattttc
     8161 ctttatatga acaggttaca accggcccgg tcgcggcatt acattacgat ggcacaacca
     8221 cctcgatgta taacgagttt ggcgacagta ctaccacgct gaccgatccg ttatggcatg
     8281 ccagcgtgag tagcttaggc tggcgtgttg actcccggct tggcgatctc cgaccctggg
     8341 cgcaaatcag ctataaccag caatttggcg agaatatctg gaaggcgcaa tcaggcctga
     8401 gccggatgac ggcgacaaac cagaacggca actggctgga tgtcaccgta ggcgctgata
     8461 tgttgctcaa tcaaaatatt gccgcctatg ccgcgctaac tcaggcagaa aataccacta
     8521 ataatagcga ctatctgtat acgatggggg ttagcgccag attttaacgt aacagtcaca
     8581 attgaaacca ttgaataaca atagttgtgg tgatagtggg tgctaactta caaataataa
     8641 atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga
     8701 tggcatttca tgccgttttt ccccaggcat aaaatgcact tcgttatggt tgtcggcaga
     8761 gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac
     8821 ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac
     8881 ctggagcctg tttaatggcg cgctttctgc caagctggat gcgccggtaa gccaggtcgc
     8941 tttctctttc ggcttgttaa gtctggggct ggcaatttca tcttctgttg cggggaaatt
     9001 acaggaacgt tttggcgtta aacgcgtcac tatggcttcc ggcattttgc taggattagg
     9061 cttcttcctg acagcgcatt ctgacaacct gatgatgctg tggttaagcg ccggtgtgct
     9121 ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt
     9181 cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc ttggtagcct
     9241 gggtttcaaa tttatcgaca cgcagctgct ggaaacggtg ggtctggaaa aaacatttgt
     9301 gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc
     9361 accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga
     9421 gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag
     9481 cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt
     9541 ggtttccgca gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt
     9601 gctgggcatt ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat
     9661 ttcgctggtg ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc
     9721 agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt
     9781 cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt
     9841 cggtatcggt agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac
     9901 cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca
     9961 gccagagcag aaaatgttgc gtgaggcgca tatttagaaa tatgttgcta ctgaaataaa
    10021 tacgaggagg gagacttcaa agtctccctt ttttatggac gcatattaaa agaacaataa
    10081 tattcatacc acaaatatat tttat
//
LOCUS       DAJTOR010000153        10048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTOR010000153 DAJTOR010000000
VERSION     DAJTOR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.153"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..206
                     /locus_tag="OO249_003313"
     CDS             <1..206
                     /locus_tag="OO249_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637497.1"
                     /translation="STLKSPSYTKSVSWQHYRHICCYRRKLTDAELEACYRYDKQFWE
                     HYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(323..1666)
                     /gene="gdhA"
                     /locus_tag="OO249_003314"
     CDS             complement(323..1666)
                     /gene="gdhA"
                     /locus_tag="OO249_003314"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5637498.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSESEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVGAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1902..2174
                     /locus_tag="OO249_003315"
     CDS             1902..2174
                     /locus_tag="OO249_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5637499.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2140..2547)
                     /gene="nudG"
                     /locus_tag="OO249_003316"
     CDS             complement(2140..2547)
                     /gene="nudG"
                     /locus_tag="OO249_003316"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5637500.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPTDIPLLEAFMALRAARAAD"
     gene            2634..3245
                     /locus_tag="OO249_003317"
     CDS             2634..3245
                     /locus_tag="OO249_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5637501.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFISTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3254..4561)
                     /gene="ynjE"
                     /locus_tag="OO249_003318"
     CDS             complement(3254..4561)
                     /gene="ynjE"
                     /locus_tag="OO249_003318"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR5637502.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4628..5281)
                     /locus_tag="OO249_003319"
     CDS             complement(4628..5281)
                     /locus_tag="OO249_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5637503.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5281..6816)
                     /locus_tag="OO249_003320"
     CDS             complement(5281..6816)
                     /locus_tag="OO249_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5637504.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPM
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCTLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALAAKWVGYVRQ
                     GLR"
     gene            complement(6789..7955)
                     /locus_tag="OO249_003321"
     CDS             complement(6789..7955)
                     /locus_tag="OO249_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5637505.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WDDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(7965..8513)
                     /locus_tag="OO249_003322"
     CDS             complement(7965..8513)
                     /locus_tag="OO249_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5637506.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8513..9220)
                     /locus_tag="OO249_003323"
     CDS             complement(8513..9220)
                     /locus_tag="OO249_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5637507.1"
                     /translation="MMKMQSRKIWYYCITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9235..9912)
                     /gene="ydjY"
                     /locus_tag="OO249_003324"
                     /pseudo
     CDS             complement(9235..9912)
                     /gene="ydjY"
                     /locus_tag="OO249_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
     gene            complement(9917..>10048)
                     /locus_tag="OO249_003325"
     CDS             complement(9917..>10048)
                     /locus_tag="OO249_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5637508.1"
                     /translation="LQLCLAGLALFILVQLAKLYARHKHVDLSASRRSPLTHPKNEG"
BASE COUNT         2400 a         2740 c         2591 g         2317 t
ORIGIN      
        1 caagcacctt aaaatcacca tcatacacta aatcagtaag ttggcagcat taccggcata
       61 tctgctgcta tcgcagaaag ttaacggatg ctgaacttga agcgtgttat cgttacgaca
      121 agcagttttg ggagcactac agtaagaaat gccgttggcc cataagaaat gttccctggg
      181 gagcatcatc cgttaaatac tcataaacgc ctgaaatttt gccgggggcg ctgcgcttgc
      241 ccggcctaca ttagagcgta actgcaatga attgtgccca tttgtaggcc tgataagcgt
      301 agcgccatca ggcatttaca acttaaatca caccctgcgc cagcatcgca tcggcaacct
      361 tcacaaaacc ggcaatgttc gcgccctgca cgtagttggt ttgctcacct tcaccaccat
      421 gctcaacaca ggcatggtgg atatccagca tgatgtgatg caaacgtgcg tcaactttct
      481 cggctttcca gcccaggcgc gcagcgtttt gcgccatttc aaggcctgac gttgctacac
      541 cgccagcatt agccgcttta cccggtgcaa acagtacgcc tgcctgctgg aacagttcag
      601 tcgcttcgat ggtggtcggc atatttgccc cttcggcgac ggctttaacg ccattggcga
      661 taagctgatg cgcggcgcca acatccagtt cgttctgggt ggcgcaaggc agggcgatat
      721 caaccggtac agaccacggc tgttggcctt cgagatagac cagaccaaat tctttggcgt
      781 aatctgccac tcgaccatcg cggctggctt tgatttcgat aagacgtgcc agtttctctt
      841 tcgtgaatcc gctttcatca actacagtgc cgctggagtc tgacgcagtg atcacacgag
      901 caccaaattc catcgctttt tcgatagcgt actgggcgac gttgccggag ccagaaacgg
      961 aaacgcgcat cccttcaaaa cccataccgt ggcgttttaa cattgcttct gtgaaataaa
     1021 ccagaccgta gccggtagct tccgggcgaa taagactacc gccaaatgaa aggcccttac
     1081 cggtgaagac gcaggcggta ttgttggaga gctttttcat catccccgcc ataaagccga
     1141 cttcacgacc accaacgccg atatcacctg ccggaacgtc agtatccgcg cccagatgac
     1201 gataaagctc ggtcatcagc gcctggcaga aacgcatcac ttcgctttcg cttttacctt
     1261 tcgggtcaaa atcgctaccg cctttaccac cgcccatcgg cagggtagtc agggcatttt
     1321 tgaaggtttg ttcgaagccg aggaatttga gaatggagag gttaactgac ggatggaagc
     1381 gcataccgcc tttgtacggg ccgatggctg agctgaactg cacacgccat gcacggttga
     1441 cctgtacctg gttgcgatca tcaacccata ccacgcgaaa ctggatcacg cgctccggtt
     1501 caaccagacg ctccagtaat gacatctggc gatattttgg attttgttca agaaaaggcc
     1561 agagtgtggt cattacttca cgaacggctt gcgcgaactc ggtttgattc gggtcgcgct
     1621 tttggacatg gttgaggaat gactccagag aatatgtctg atccatagat ataaaaccct
     1681 tatatattaa tacgattgtg cttatgttgt ttatgtcatg tgcttttgca gttttcgact
     1741 ataccatcaa gaatgtgacg gtaagcaaga aaaaaatgac ccaggaaagc aaattaataa
     1801 cgagagtaat ctcataacca aatgcgataa ctcagacgct aaagttttgc cacccggcta
     1861 ccgttatcag ttgcagagac aataacgtta aggagttccc tgtgagtcga gcattgttcg
     1921 ccgtagtatt agcatttcca ttaattgcac tggctaaccc gcattatcgt ccggacgtgg
     1981 aggtaaacgt accgccagaa gtgttcagtt caggcgggca gtcagcgcag ccctgtactc
     2041 agtgctgcgt ttatcaggat caaaattatt ctgaaggggc ggtaattaaa gccgaaggaa
     2101 tactgctgca atgccagcgg gatgacaaaa cccttagcac taatccgctg ctctggcggc
     2161 gcgtaaagcc ataaacgcct ctaatagtgg aatgtcagta ggggccagcg gatattgcag
     2221 cgcctcttca ggtgagcacc agaccagcgc ctgatgttca tgtgcctgta acgtcccgtg
     2281 gaagtcgggt acgtgccagg catgaagatg gataatccgg cccgaaactt ctcgctgatg
     2341 gctggcaaca tactcaccca cagttgcttc gatgcccaat tcttcgttta actcacgcac
     2401 cagtgcctgc tgctggcttt catccagctc gactttacca ccggcaaact cccataatcc
     2461 cgcctgatcg ctctgggcgg ggcgttgcgc gagtaaaatt ttgccatcac gttcaatgat
     2521 ggcggcaaca acttcaatca ttttcataaa ttacacacgg gcagataagc gaaaaatgcc
     2581 ataatatccg cactgttgct caatcagaaa ggctttatat ttaaggagtg cctgtgctag
     2641 accgccatct tcatccccgg attaaaccgt tgttgcatca gtgcgtgcgg gttcttgata
     2701 aaccgggcat tacgccagat ggtttaacat tagtgggatt tgccatcggc gtgctggctc
     2761 tgccgtttct ggcgctgggc tggtatctgg cggcgctagt cgtcattttg ttgaacaggc
     2821 tgcttgatgg tctggacggt gcgctggcgc ggcgcagaga gcttaccgac gcgggcggct
     2881 ttcttgatat ttctctcgat tttctctttt acgcgctggt gccgtttggc tttattcttg
     2941 ctgcaccgga gcaaaatgcg ctggcaggcg gctggttgtt gtttgcgttt atcagcacgg
     3001 gcagcagttt tctcgccttt gctgcactgg cagcaaaaca tcagattgat aaccccggtt
     3061 atgcacataa gtcgttttac tatctggggg gattaaccga aggcacagaa acgatcttac
     3121 tgtttgtgct gggatgttta ttccctgcat ggtttgcgtg gatcttcggt gcgttgtgct
     3181 ggatgacgac ctttacgcgg gtgtggagtg ggtatctgac gctgaagtcg ctccagcgtc
     3241 agtaattgca atgttatttg ctactgtccg ggccgcgttc accggttgct accggatttt
     3301 ttggatcgct gctccattcg taccagccgc cgtcatacac ggaaacattc ttccaaccca
     3361 tggcgcgtgc gtacataaag gtttcggacg cgcgccagcc ggtgccgcag tagaatgaaa
     3421 cttgctgctc tggtttgata ttccacgctt tccacatagc ggtaatatca tcggcgctgc
     3481 gcatggtgcc atccgggtta tgaaaatctt ccatatgcgt cgagtcgcta ccagcgtgtc
     3541 cccaacgtgc tccggctatt tcaccttttg gtttaatata gctgtaaccg ctggtcgtac
     3601 cgataaattc tggccacgaa cgaatgctca ccagcgatgc atcctggcga tgcagcagtc
     3661 cacgggcctg ttccatatcg agcatcaact gcggttgtgc cgggatcttc acgccgaaat
     3721 ccggttccgc tttcactttc ggtggcgttc ctcgctcaac aggcagtccc gcgtcggacc
     3781 aggtttgcca gccgccatcc agcagacgca catctttcac gcctgcgtag agcataattt
     3841 gcgccacacg cgctccagcg tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc
     3901 gaatgccgtg ttttgccagc atcgctttta gttgttcatc agaaactttg ttccacagcg
     3961 gttcgctttc cacttcgttg gtatcgatgt agtcagcacc gggaatatgg ctgataaggt
     4021 aaagcttagg agcgccccag gccgcttcaa tgactttcca gtcaccggca ggtttcgccg
     4081 taacctcttt accttgttgc aggtcgtgca gccattgcgg ataaaccagc tgctcaaagt
     4141 gcggcagttt ttgtagacgg gaaggttcgc ttagcgcgtc actcagaatg gagatatgcg
     4201 taaaacctgc tttttgcagt cgcgttttga cggcgtcgac atctttgtca ttaccgtaca
     4261 gcgccaccgg ggcatcggct ttcaggttat gttgcttgat ccacgcgttg agctgttcgg
     4321 tgctcatttt gtcgagccag ctggcagaga ggtttaaggc ggcaggttca tgaccagaag
     4381 ggccatttaa ggtttgtggc cagccgttat aaaacgcgct ggggcgagta tctatcgctt
     4441 tgccattttg ttgttgaagc tggtcaagtg taagaggctt cgccagttca gcggcccacg
     4501 aagaagcgca agccagccct aaagccattg ccagcgcggt catttgagaa acacgtttca
     4561 tcgaaaatcc cgacattgta gttttgagtg cgccattctg aactgacgca ttgagaaaaa
     4621 ctttgcgtta tcgcagtttg ttgtaatttt ctgaccactg cgccatatcc agaacagaac
     4681 tatcagcagg aacatcctgg agatcgtgcg ttacctgaac gacggggatc gccagttcgc
     4741 gaacttcgct gaacacccac tggcgaaaat tatcgcgcag agccacatca agacggctga
     4801 atggctcatc caggagtaac gcttttggct gagcgagaag ggcgcgcagc agggccaccc
     4861 gcgctcgctg accgcctgat aatgtcgcag ggtcctggtg ataagtttca gccagtcccg
     4921 ctcgatcaag cgcgtctttc acggcattgc gcctggcagt ccccttgagc gtcgacggta
     4981 gcgccagcag taaattttgc ccgacgctga actggtcaaa taacagtgca tcctggaaaa
     5041 gaatgccaat ctgacgctgt gcggtgggta gcatgtcaat ccgttgctca ttgagccata
     5101 gctcacctgt acaagaaaac tgtccagcca gtgcaccaat catccatgaa aacagagtgg
     5161 attttccaca gccagacggc cccattaacg tgacgatgtc acctttatca accgtaaagt
     5221 taacgtttgt cagcaagcgg ctttctggta aacgtagcga aacatttttc acgcagagca
     5281 ttagcggagt ccttgtctga cataacctac ccattttgcg gctaacgcgg tcagggcaaa
     5341 aataataagc ggtaacaaca gttgccataa agcctgggcg gcgagaatac cgttgctgcc
     5401 gccgctgctt aatgccaccg cctcagtggt gagcgtcgga aaacgccccg cgcccagcca
     5461 cagcgtcggc atatactgcg caatactgac tgaaaatccc accgcgaagg caatcagcac
     5521 agggcgcaac atgagtgtgc atttcacgta aaagaagatt ttggcccgcg accagcccag
     5581 tgtttgcgca atcaaaatta accgtgaatc aatgcgctgc caggcgggtt gcaggataaa
     5641 cagcatccac ggcattaccc acagcagatg cccccagacc accgccgtcc agcttccatc
     5701 caggttcagc catagcgcca gcgtgtactg gcccgccacc agtggcagag cgggcaatag
     5761 aatgggcaac catagccaca actggcgacg ctgtggcccc cattccagcc acagcagtaa
     5821 caggagcaaa gcgatgaatg ttgctaccag ccccattgtc agactgttga tgagtgcttc
     5881 actattgatc gtcgactgat ccgcgaggat cgccagcaga accacacaca gcacaccggt
     5941 taagggtaaa aaactcgcca gcgtagtgcc tggtaataaa ggtgtggcag gcttgcgaat
     6001 gccatctacg cggggaatag tgcgccgcca gctgcgccat agcagatagc tcagcaaaac
     6061 gtaggcggcg agtaacagca tcagcagcag actggcgagt gcgcctttcg tttgttgatc
     6121 cgcgtcgccc tgagttaacc actgccagct aattactgcc agcatcggtg gattacctgg
     6181 cccaagaata atcgccacat ccacgaccga aagcgaccag gcaacaatcg ccagcatcgc
     6241 cattgccagc gaaggcgcga cggagggcaa cagcagccag ttcaggcatt gccagcggct
     6301 gtagcccagc gaatccagca caatgacctg ctgcaacagc cgtttttcgc tcaatactgc
     6361 cgctaagatc cacagcagaa atgcgctttc tttcactgca agggtgaggc ccagaccgat
     6421 gccaaatcgg tccattggcg gagtgaaata cgggaaatag tcatatagca gccctccgtc
     6481 agcaaagagc agcagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg
     6541 cagacgggcg cacatacgct gccatttcgg cccaggccat agcgccacaa tcaccagcag
     6601 ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc
     6661 atgcggtaac tgtggatcgg caaataacgc ctgccagtgt gtcaacgaca acgcaggcga
     6721 tatcagcgtg agagctgccg ggatcagcgg tgcataaatc accgccacca tcgcccacag
     6781 caaaaagatt aatgcgtacc gtaacggcgt aaccattctt gttccagtgc attcacccat
     6841 cctgcgtgcg gttcagccag taccggcggc agatcctgcg gcattcttga ttgcaatatt
     6901 tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag aaggatcgtc ccagacaacg
     6961 ggatctgctt tacgcagttg cgcatcgggt gagagcagaa aattggcaac taccttcgcc
     7021 gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat gcacgttgcc aatcatcccc
     7081 tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc cgctggcgat tttttgctgc
     7141 gcatgcgcgg ggttaaaggt cagcgacagg cgcaatgtgc cggctttcag cagagcatcc
     7201 atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg atgcagtgca
     7261 tcaagatatt gccacaaggg cgcagtgaca cgggcgaaag tcgcatcgtc cggcgcttct
     7321 tttaatgcgg cgggatctgg cgtcagcata atcagcaact gttcaagaaa cgccgtgccg
     7381 gtaaagtccg gtgggcgcgg ataagtaacc gtgccgggat tggctttagc aaactccagt
     7441 aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaacgtcagt
     7501 tgtgcgccgc cccagggcga ttccgcacct tgtgtcggca ctgaaaaatc ttcccgcacc
     7561 ggcagctgtg tgtcgacata gcgccagttg ggcagagtct ccgcccagcc cgtttgtaat
     7621 aaattggcct cttttaaggt gcggaagttt tcgccgttca cccagagcag atccaccgag
     7681 ccgcccgttt tacgaccggc agcagcttcg gtctgaatgc gcttcaccgc gtctgcggca
     7741 tccgcgagac gaacaatctt caggtttata gcgtaatggg ttttcatctc gccgctaacc
     7801 cagtcgagat agcggttaat tgcggtatcg ccgccccagg cgttaaacca gacggtttgc
     7861 cctttggcct catttttaat ttcctgccag tcactggcat gtgttgccaa agaaaacagc
     7921 gataacaatc ccagcaacca cccacaatgg cgcatcttct ctccttaaga acgtttttcc
     7981 ggaatacggc acagcccctg agcgggaatg tccatggcag aattaaaacg agccgacagg
     8041 ttttgcaggc cgatcaatgc cgtcagttcg gtgagcgcct gagcgtcaaa atgcgcagcc
     8101 agtcgggtac gcagggcatc atcgactgtt ggcggtgtga cgcttgcggc ttcggcgtac
     8161 tccagcgcca gccgttcttc atcgctaaag agcgggcttt gccgccagtc agccactgcc
     8221 agcagtttat cggtgctgcc ggtacgctcg gcgactttca tactggtgat atccacacaa
     8281 aactcacaca ggcacatttg cgcaatgcgc gcgctgacaa gcgatcgcac taccggatcg
     8341 agcggtgagc gtttgcgctc cagccaccca acaaacattg acaccaaata aaagataaac
     8401 gggatccgcc cccaccagcg aatcgggctt agcacctcgc cgtaatggcg acgatgcagc
     8461 cacgcctgcg gacgtaaaat gaaaggaatt ttgctaagcg gcggtaaacc catcagtcat
     8521 tcctcttctg gcgctcaagc catattttct tcgccataaa aatcaccacg gtcagggcaa
     8581 acagaataaa cagtccggtg acaaaccaga aagtaccgcc tgttaacatg ctgcccgccc
     8641 aggaataaac aatagtcgcc ggtaattgac caagcccggt ggcgataaaa aacgagcgaa
     8701 aacgtattga agtcagaccg gcagcatagc tgattggatc gaaagggaca aaaggcaata
     8761 accgacagac caggatggtg tgtttgccgt agcgagtgaa aaagccgtcc atactgtcaa
     8821 gcacggtttt gccggttaat ttttccacca cttcgcggcc catcactctg gcgataaaaa
     8881 agcacagcgc cgcgccggcc atcgaactgg tccacgacag caagccgccc cagaacgcgc
     8941 caaacagcga cgcattggca aaggtgatca aaaacgcagg tagcggtgca gcaatggcct
     9001 gtaaaatcat caacaagaac gagacaactg ccgccagtgc gccgtaagac tgaataaagc
     9061 gttctatacc ctgttggtcc acggcggcaa acgccgcaac gctgcgattg ataaactcat
     9121 ggacgccggg aagcagcgcc catgcgagca gcatagcgaa caacaggatg atgagggtta
     9181 tacagtagta ccagatttta cgcgattgca tcttcatcat cctttatcct ggctttattc
     9241 ggtgatttta aaggtaaccg ttgccagcgt gttatccgcc gggagaactg aggcattgcc
     9301 tttgaatttc acttcgccgc gtttttccac cgcaccataa gtgtatgttg cattgctgac
     9361 gatgccgacc gggcagctat ccagacacac cagtcaaccc gttttcttct cttctgctgc
     9421 cgttaaatta ccgccaaagc gcatgtccag tttcttgcca ttactgtcaa caatcacttc
     9481 atcgaaggaa tacgcttttg ccgccccttg ccagttgacc gaaatatcca gtttgctgcc
     9541 tgtgacatga gtcgtttctt tattatccat cgtcatgttt tcgcccggcg taccacctgc
     9601 ctctttcagg gcttcataaa atgctttcgg ggtcgcataa gccataaaca gcgatttatg
     9661 tccgttggag ccatctttaa acacaatacc gtgacgagtg tcgtcggtga ggaaacgacc
     9721 attaatttgc accagcatgg ttaccgtatg gttattggca tcgacgcgca gcggttggct
     9781 gttcgaaagt ccgtcgtatt ccacttttgc cgccgaattc tctttttgat cgcagccgct
     9841 aagcccggca acagccagta agcagagtgc agccagtcct tttttccatg acactgaata
     9901 atgttgcgac atcgttctat ccttcatttt ttgggtgagt aagcgggctg cggcgcgaag
     9961 cagacagatc cacatgtttg tgtcgggcgt agagttttgc gagctggacg agaataaaca
    10021 gcgccaggcc cgccagacag agttgtaa
//
LOCUS       DAJTOR010000154         9955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTOR010000154 DAJTOR010000000
VERSION     DAJTOR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.154"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            259..456
                     /locus_tag="OO249_003326"
     CDS             259..456
                     /locus_tag="OO249_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637509.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(406..1599)
                     /locus_tag="OO249_003327"
     CDS             complement(406..1599)
                     /locus_tag="OO249_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5637510.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1735..3459
                     /gene="iucA"
                     /locus_tag="OO249_003328"
     CDS             1735..3459
                     /gene="iucA"
                     /locus_tag="OO249_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5637511.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3460..4407
                     /gene="iucB"
                     /locus_tag="OO249_003329"
     CDS             3460..4407
                     /gene="iucB"
                     /locus_tag="OO249_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5637512.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4407..6149
                     /gene="iucC"
                     /locus_tag="OO249_003330"
     CDS             4407..6149
                     /gene="iucC"
                     /locus_tag="OO249_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5637513.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6146..7483
                     /gene="iucD"
                     /locus_tag="OO249_003331"
     CDS             6146..7483
                     /gene="iucD"
                     /locus_tag="OO249_003331"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5637514.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVITLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7489..9684
                     /gene="iutA"
                     /locus_tag="OO249_003332"
     CDS             7489..9684
                     /gene="iutA"
                     /locus_tag="OO249_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5637515.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWIIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2396 a         2564 c         2644 g         2351 t
ORIGIN      
        1 cataaaaaac atgtcagtga aaaagaaacc tggcgttggg ttgaaggata taataaagca
       61 acagaactgg ctcgccaatg ccctgatacc catgtattgt caatttctga tcgtgaaggc
      121 gatttttatg acctgtttga acgcgcagag cagactcccg gaataaaagc tgactggctt
      181 gtcagaatga aatttaataa cagagcaacg ctgaatatta atggtaaacg ggatcatagg
      241 ttgttgcacg aaagaatcat ggagattaca ccgcagcaac tggttgaatt cactatacct
      301 gatgggcggg ggcagcaagc cagaacactg cctgacatgc tttcatcaga cgcaggtcct
      361 ctctcatatc ctctgattta ctccagtaag gtgatgatca tatctttatt tgatagtacg
      421 gtaatgccag ataaatacag acacaatggt aaataatata gttgcaaata atgctgccat
      481 aatcagcgcg attgcgacac ctgttttcga taccagcggc accagcaaag cgcacagtcc
      541 atagccaagt gtgtgactgg tagcgagcca gcccgcgcct gcgccatcag ccagcttatc
      601 attcagtaga agttgataag cgggtgttgc cagagctgct ccaaatgaca gcactgcaca
      661 gcccgggtaa aacaaccata gctgttcgga gagcatgata gccagaccac cactcatcaa
      721 cactccggca ctcaggagca gggccaccgg agtcaggcgc tgcggacgga ctaccccgaa
      781 ctgcgcgata agcgcagcta ctgcggacag acccaacagc caggccacct gttggctaat
      841 ggcggtggta tccgtgacga actggcgagt aagggcaggc gaaagtccaa gctgcatcat
      901 gctcaccgcc gcagccagta atattgcgca aagcagataa ggcagacaat cccgcttcag
      961 actgacgctc ttgcactccg gtgtgggctg tggtggtgtg ccgggcaacc gcagcagcat
     1021 cagcagcgcc agcactggtg ctgccatcag tagccccagt ggcgccagag gatgaatggc
     1081 cagcattgct gccgcgcaca gcggcccgaa tagcctgccg caactcaggc cggagctgat
     1141 ggttgccaga gcggccatgc gattcccttc tccagctctc tgcaatgccc agacctgaca
     1201 ggctggcacc attgccgaca cggtcagccc gtaggcgatc cgggcgatga ttaatattcc
     1261 cagtcccacc gctgttgtta tggcgctggt agccatcagc acgcttccca gccctagcag
     1321 ggtaaagctt atgccatatc ctcccagagc ccacaacacc acaggtttac tgccagtacg
     1381 ggaaatttgc tttccccacc acggggacga tggcagaaac agcattgagc caagcatcag
     1441 cagggcagcc catacagaca gactcagatt tgtctgtatg accagaacag ggagcacaac
     1501 cagcagaccg ttctgtccga taccgagaag cccggcactg aacgcaagtg gccagcagga
     1561 cagtgttttt tggggcgtat cttcgatccc tggtgacatt ataatgcttc aactccatgt
     1621 attaattgtg tttatttgta aaattaattt atctgacaat aacatttatt gttgataatg
     1681 agaatcatta tcgacataat tgttattatt ttactgtgtg ggagctgttt gactatgacc
     1741 ctgccctctg aaaaaccagc cacagatgtg gctgcgcagt gcttcctgaa tgcactgatt
     1801 cgtgaaacca cagactggaa actgacagaa tacccgccag acgaattgct catcccgctg
     1861 gatgagcaga aatcgctcca tttcagagtg gcttatttct ccccaaccca gcatcaccgc
     1921 tttgcatttc ctgcacgtct ggtcacggca tcaggcagtt atcctgtcga ctttaccact
     1981 ctctcccggc ttattattga taagctacgc catcaactgt ttctgcccgt tcccctctgc
     2041 gaaactttcc accagcgcgt gctggaaagc catgtccata cgcaacaggc aattgatgcc
     2101 cgtcatgact gggccgctct gcgtgaaaaa gcgttgaatt ttggcgaggc tgagcaggcg
     2161 ctgctgacag gacacgcttt ccaccctgcg cctaagtctc atgaaccgtt taaccggcgg
     2221 gaggctgaac gctacctgcc tgatatggca ccccacttcc cactgcggtg gttttcggtg
     2281 gataaaacgc aaatcgccgg tgaaagtttg catctcaacc ttcaacagcg gctgacgcga
     2341 tttgccgcag agaatgcgcc tcagttactc aacgaattaa gtgacaacca atggctgttc
     2401 ccgttgcacc cgtggcaggg ggaatatctt ttgcagcagg ggtggtgcca ggcacttgtt
     2461 gctaaagggc tgattaaaga cttaggtgag gccggcacgt cgtggctgcc gaccacctct
     2521 tcccgttccc tctactgtgc caccagccgc gatatgatca agttctccct gagcgtacgg
     2581 ctgaccaact ccatccgtac tctgtccgtg aaagaagtga agcgtgggat gcgcctggca
     2641 cgcctggctc aaaccgacgg ctggcagatg ctacaggtcc gcttcccgac tttccgggta
     2701 atgcaggagg atggctgggc cgggctgctc gatcttaacg gcaacatcat gcaggaaagt
     2761 ctgtttgccc tgcgtgaaaa tctgctggtg gatcagccga aaagccagac taacgtactg
     2821 gtctccctga ctcaggccgc acctgatggc ggtgattcgc tgctggtttc ggcggtaaaa
     2881 cgcctgagcg atcgcctcgg tatcactgtg caacaggccg cccatgcatg ggtcgatgca
     2941 tactgtcagc aagtgctaaa gccgctgttt acggctgaag cggattacgg cctggtgctg
     3001 ctggcgcatc agcaaaatat tcttgtccag atgcttgggg atctgccggt cggatttatt
     3061 taccgtgact gtcagggcag cgcttttatg cctcacgcga cagactggct cgattctatt
     3121 ggcgaggcgc aggcggaaaa tattttcacc catgagcagt tgctgcgcta ttttccttat
     3181 tacctgctgg ttaactccac ttttgctgtg accgctgcgc tgggggctgc cgggctggac
     3241 agcgaatcga atctgatggc tcgtgtacga gcatcgctgg ctgaagtgcg tgatcaggtg
     3301 actcataaaa cctgtctcaa ctacgtgctg gaaagtccgt actggaacgt aaaaggtaac
     3361 tttttctgtt atctgaacga tcataacgag aacaccattg ttgacccttc ggtgatctat
     3421 ttcgatttcg ctaacccgct gcaggctcag gaggtctgaa tgtctgaggc aaacattatt
     3481 cacagcagat atggactgcg ctgtgaaaaa ctcgacaagc ccctgaatct tggctgggga
     3541 ctggacaata gcgcggtgtt gcactgtccc ggggagctgc cgacagggtg gctgtgcgat
     3601 gcgctggatc agatatttat cgccgcacca caactgtcag cagtggctct cccctgggcc
     3661 gaatggcgtg aggagccaca ggcgctgacg ctttttgggc aggtaaaaag cgacattatc
     3721 catcgtactg ccttctggca gttaccgtta tggttgagtt caccggcaaa ccgggcctcc
     3781 ggtgaaatgg tttttgatgc agagcgtgag atttatttcc cgcagcgccc accccgtcca
     3841 cagggtgagg tttaccgccg ttacgatcca cgtattcgca ggatgctgag tttccgcatt
     3901 gccgatcccg tttctgatgc agaacgtttc acccgctgga tgaatgatcc gcgcgttgag
     3961 tatttctggg agcaaagtgg ctcactggag gtacagaccg cctatctgga gcgccagctc
     4021 accggtaaac atgcattccc gctgattggg tgcttcgacg atcggccatt cagctatttc
     4081 gaaatctact gggcggcgga agaccgcatt ggtcgtcact attcgtggca gccctttgac
     4141 cgtggcctgc atctgctggt tggtgaacag caatggcgcg gggcccacta tgtgcaaagc
     4201 tggctgcgcg ggctgacaca ttacctgctg ctggatgagc cccgtacgca gcgcacagta
     4261 ctggagccac gcaccgataa ccagcgcctg ttccgccatc ttgagcctgc gggataccgg
     4321 acaattaaag agttcgactt cccacataag cgctcgcgca tggtgatggc ggatcgtcat
     4381 cacttcttca cggaggtcgg tctgtaatga atcacaagga ctgggatttt gttaaccgtc
     4441 ggctggtggc aaaaatgttg tctgagatgg agtatgagca ggttttccac gcggaatctc
     4501 agggcgatga ccactactgc attaacctgc cgggagcaca atggcgcttc atcgctgaac
     4561 gtggtatctg gggctggctg tggattgatg cccaaacgtt gcgctgcacg gacgaaccag
     4621 tactggcgca gacgctgctg atgcagctaa agccggtgct gtcaatgagt gatgccactg
     4681 ttgcagagca tatgcaggat ttgtatgcca cgctactggg cgacctgcaa ctgctgaagg
     4741 cccgtcgcgg actgagtgcc agtgacctga ttaatcttga tgccgatcgt ctgcaatgcc
     4801 tgctgagcgg tcatcctaaa ttcgttttta ataaaggtcg ccgtggctgg ggcaaagagg
     4861 cgctggaacg atatgcgcca gagtatacca acaccttcag actgcactgg ctggcggtaa
     4921 aacgtgaaca tatgatctgg cgctgcgata acgatctgga tattcagcag ttgttgactg
     4981 ccgcaatgga tccgcaggag tttacacgct tcagtcaggt ctggcaggaa aacgggctgg
     5041 atcacaactg gctaccgctg ccggtacatc cgtggcagtg gcagcaaaaa atcgccacag
     5101 acttcatcgc tgattttgtc gaaggcagga tggtgtctct cggcgagttt ggcgaccagt
     5161 ggctcgccca gcagtcgctg cgtaccctga ccaacgccag ccgccgggga gggctggata
     5221 tcaagctgcc gctgaccatc tacaacacct catgctaccg ggggatccct ggcagatata
     5281 tcgctgccgg accactggct tcacgctggc tgcaacaggt ttttgcgacc gacgccaccc
     5341 tggtgcaaag cggcgcagtg atccttggtg aaccggcggc aggctatgtg tcccatgaag
     5401 gctatgccgc gcttgcccgg gctccctatc gctaccagga aatgcttggt gttatctggc
     5461 gggagaatcc gtgccgctgg ctgaaaccgg atgaaagtcc tgttctgatg gcaacactga
     5521 tggagtgcga cgaaaacaat cagccgctgg caggcgcata tatcgaccgc tccggactgg
     5581 acgctgaaac ctggcttacg caattgttcc gggtggttgt ggttccgctg tatcacctgc
     5641 tttgccgcta cggtgtcgcg cttattgcac acggacaaaa tataacgctc gccatgaaag
     5701 agggggtacc acagcgtgtt ctgctgaaag acttccaggg cgatatgcgg ctggtgaaag
     5761 aagcgtttcc cgaaatggac tctttgcctc aggaggtgcg tgatgccaca tcccgtctga
     5821 gtgcggacta cttaatccat gatttgcaga cgggtcactt cgtgacagta ctgcgtttta
     5881 tttcgccact gatggttcgt cttggcgtac ctgaaaggcg attttatcaa ctgctggcag
     5941 cagtgttgag tgattacatg aacaaacatc cacaaatggc agagcgtttt gcgcttttct
     6001 cactcttcag gccacaaatc attcgcgtgg tgctgaaccc ggtaaaactg acctggccgg
     6061 acctggatgg cggcagtcgc atgctgccga attaccttga gaatctgcaa aatccgctgt
     6121 ggctggtaac tcaggaatat gaatcatgaa aaaaagtgtc gattttattg gtgtcgggac
     6181 agggccattt aatctcagca ttgctgcatt gtcacatcag atcgaagaac tgaactgtct
     6241 cttctttgac gaacatcctc atttttcctg gcatccgggt atgctggtac cggattgtca
     6301 tatgcagacc gtctttctga aagatctggt cagtgcagtt gcacctacaa atccctacag
     6361 ctttgttaac tatctggtga agcacaaaaa gttctatcgc ttccttacaa gcaggctacg
     6421 tacagtatcc cgtgaagagt tttctgacta tctccgctgg gctgctgaag atatgaataa
     6481 cctgtatttc agtcataccg ttgaaaatat tgatttcgac aaaaaaagtc gattgtttct
     6541 ggttcagacc agtcggggag aatattttgc ccgcaatatc tgccttggta caggaaaaca
     6601 accttattta ccaccctgtg tgaagcatgt gacacaatcc tgtttccatg ccagtgaaat
     6661 gaatcttcgt cggccggacc tgagtggaaa acggataacc gtggttggtg gaggacagag
     6721 tggtgcagac ctgttcctta atgcattacg cggggaatgg ggagaagcgg cggaaataaa
     6781 ctgggtctca cggcgtaata attttaacgc actggatgag gctgcttttg ctgatgagta
     6841 ttttacacct gaatatattt caggcttctc cggactgaag gaagatattc gccatcagtt
     6901 actggatgag cagaaaatga catcggatgg catcactgcc gattctttac tgaccattta
     6961 tcgtgagttg taccaccgtt ttgaagttct gagaaaacca agaaatatcc gtctgctacc
     7021 cagtcgctcg gtaataactc tggaaagtag tggtccgggc tggaggttat tgatggaaca
     7081 tcatctggat cagggcagag agagcctgga aagtgatgtg gtgattttcg ccacaggtta
     7141 ccgttctgca ttgccacaaa tacttccctc actgatgccc ttgatcacca tgcacgataa
     7201 gaacaccttt aaagtgcgtg atgacttcac tctggaatgg agtggcccga aagaaaataa
     7261 catctttgcg gtcaacgcca gcatgcaaac tcatggcatc gccgaacccc agctcagcct
     7321 gatggcctgg cgatctgcac gtattcttaa tcgcgtactg gggcgtgatt tattcgatct
     7381 cagtatgccg cccgcactga ttcagtggcg cagcggcagc cggaaaaaac cgcagccgga
     7441 ggctgcttct ttaactcact atacagcaaa tattcaggaa taacgatgat gcgcaaaaag
     7501 tatatgcccc gggctcttgg tccgctgctt cttgtcgtgc tgtcaccagc tgtcgcccag
     7561 caaaacgatg ataatgagat catagtgtct gccagccgca gcaatcgaac tgtagcggag
     7621 atggcgcaaa ccacttggat tatcgaaaat gccgaactgg agcagcagat tcagggcggt
     7681 aaagagctga aagacgcact ggctcagtta atccccggcc ttgatgtcag cagccagagt
     7741 cgaaccaact acggtatgaa catgcgtggc cgcccgctgg ttgtcctgat tgacggtgtg
     7801 cgcctcaact cttcacgttc cgacagccga caactggact ctgtcgatcc ttttaatatc
     7861 gaccatattg aagtgatctc cggcgcgacg gccctgtacg gtggcgggag taccggaggg
     7921 ttgatcaaca tcgtgaccaa aaaaggtcag ccggaaacca tgatggagtt tgaggctggc
     7981 acaaaaagtg gctttaacag cagtaaagat cacgatgagc gcattgccgg tgctgtctcc
     8041 ggcggaaatg accatatctc cggacgtctt tccgtggcat atcagaaatt tggcggctgg
     8101 tttgacggta acggcgatgc caccctgctt gataacaccc agaccggcct gcagcactcc
     8161 aatcggctgg acatcatggg aaccggtacg ctgaacatcg atgaatcccg gcagcttcaa
     8221 ctgataacgc agtactataa aagtcaggga gacgacaatt acgggcttaa tctcgggaaa
     8281 ggcttttccg ccatcagcgg gagcagcaca ccatacgtca gtaaggggct gaattctgac
     8341 cgcattcccg gcactgagcg gcatttgatc agcctgcagt actctgacag tgatttcctg
     8401 ggacaggaac tggtcggtca ggtttactac cgcgatgagt cgttgcggta ctacccgttc
     8461 ccgacggtaa atgcgaataa acaggcgacg gctttctcct cgtcacagca ggataccgac
     8521 cagtacggca tgaaactgac tctgaacagc caactgatgg acggctggca aatcacctgg
     8581 gggctggatg ctgagcatga gcgctttacc tccaaccaga tgttcttcga tctggctcag
     8641 gcaagtgctt ccggagggct gaacaaccat aagatttaca ccaccgggcg ctatccgtca
     8701 tatgacatca ccaatctggc ggccttcctg caatccagct atgacattaa tgatattttt
     8761 accgttagcg gtggcgtacg ctatcagtat actgagaaca gggtagatga tttcatcgac
     8821 tacacgcagc aacagaagat tgctgccggg aaggcgatat ctgccgacgc cattcctggt
     8881 ggttcggtag attacgataa ctttctgttc aatgctggtc tgctgatgca catcaccgaa
     8941 cgtcagcagg catggttcaa tttttcccag ggggtggcat tgccggatcc ggggaaatat
     9001 tatggtcgcg gcatctatgg tgcagcagtg aacggccatc tttccctgac aaagagcgtg
     9061 aacgtcagcg acagtaagct ggaaggcgtg aaagtcgatt cttatgaact gggctggcgc
     9121 tttaccggtg acaacctgcg gactcaaatc gcggcatatt actcgctttc caataagagc
     9181 gtggaaagga ataaagatct gaccatcagt gtgaaggacg acaggcgccg tatttacggc
     9241 gtggaaggtg cggtggacta cctgatcccg gatactgact ggagtaccgg tgtgaacttc
     9301 aatgtgctga aaaccgagtc gaaagtgaac ggtcaatggc aaaaatatga cgtgaaggaa
     9361 tcaagtccat cgaaagcgac agcttacatt aactgggcgc cggaaccgtg gagtctgcgt
     9421 gtacagagca ccacttcttt cgacgtaagc gatgcagagg gtaacgatat taatggttac
     9481 actaccgtcg attttatcag tagttggcag cttccggtgg gaacactcag cttcagcgtt
     9541 gagaacctct tcgaccgtga ctataccact gtctggggac agcgtgcacc tctgtactac
     9601 agcccgggtt acggccctgc ttcactgtac gactacaaag gccggggccg aacctttggt
     9661 ctgaactact cagtgctgtt ctgacgggga tgcttttgca acaagggtat gctgtaaaca
     9721 acatggtcct tggtggccat gttgtttaca gccagtataa tatacagcct taacaagata
     9781 ttccgtctcc ggaatggact gttcaccact gaataacatt atttctgttt tatggatctt
     9841 tgactgaaat ttatcgcata tcctcatttc attgtccgtc tgaaaactaa ccttacaagt
     9901 aagcgtcgtc tcagcaccgt ctggcagatc ctgaaattcc tgagaggatg atctg
//
LOCUS       DAJTOR010000155         9863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTOR010000155 DAJTOR010000000
VERSION     DAJTOR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.155"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO249_003333"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO249_003333"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5637516.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO249_003334"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO249_003334"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5637517.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3389)
                     /locus_tag="OO249_003335"
                     /pseudo
     CDS             complement(2698..3389)
                     /locus_tag="OO249_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3519..5279
                     /locus_tag="OO249_003336"
     CDS             3519..5279
                     /locus_tag="OO249_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5637518.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5685..6542
                     /gene="focA"
                     /locus_tag="OO249_003337"
     CDS             5685..6542
                     /gene="focA"
                     /locus_tag="OO249_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5637519.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6597..8879
                     /gene="pflB"
                     /locus_tag="OO249_003338"
     CDS             6597..8879
                     /gene="pflB"
                     /locus_tag="OO249_003338"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5637520.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9071..9811
                     /gene="pflA"
                     /locus_tag="OO249_003339"
     CDS             9071..9811
                     /gene="pflA"
                     /locus_tag="OO249_003339"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5637521.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2528 a         2482 c         2352 g         2501 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtttc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgtccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgaag atggaataat ccagtatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgagg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgttttccc ccgctttcat atgaatgatt tcactacaac gaatacaggc atagtccgcc
     2821 gactccggca ccactttgta acgctgcgta caatcactgg gtaaaataag taaacccggt
     2881 ttcaccttgt catcttcaaa gaaatagaca ctgatttgcc cgttggtttc gagaatcgcc
     2941 agacgtacct gccccagctg ctccacgcca cgcaatcgta gctccataaa gaactcaaat
     3001 tccgtcatgt tggagttatt gagtttcgac caggccagct cgccatcttc aataataaca
     3061 accggcttac cttccagtag atcttccagt ttttcactgt gcgccatcaa ccacattacc
     3121 aggcggtata acaacgccag agtgataaag acgataagta ccggcaccat cggcacatca
     3181 tcataaaacg ccacatctcc cgccgctgaa cccagcgtga gaatgattaa cacttcaaac
     3241 agcgacatct gtcgcacacc gcgtcttccg gtcattttga ggaacaaaaa aaccagtaca
     3301 aaggtataaa gactacgtag tgcaacttcg ccaaggaaat caaaaggcac tttatcaaat
     3361 gccatacggt ggagatcgaa cgccttcatt tttatgctct tatcgtggtt tattcgctaa
     3421 aagcatagtc aacagttcat cggcatggcg gcggtgatag cgcctttgag ataaaacccg
     3481 tattattgcg cgctttccgt acgactaaag tgattttcat gacgcaaaca tttatccccg
     3541 gcaaagatgc cgctctggaa gattccatcg ctcgcttcca gcaaaaactt tcagacctcg
     3601 gctttcagat tgaagaggcc tcctggctga atcctgtgcc taacgtctgg tctgtacata
     3661 ttcgcgacaa agagtgcgca ctgtgtttta ccaacggtaa aggcgcaacc aaaaaagcgg
     3721 cgctggcttc tgcacttggt gaatatttcg agcgtctctc aaccaactac ttttttgctg
     3781 atttctggct gggcgaaacc atcgccaacg gtccgttcgt gcattatccc aacgaaaaat
     3841 ggttcccact gaccgaaaat gacgatgtgc cagaaggact actcgatgac cgtctgcgcg
     3901 cgttttatga tccggagaat gaactgaccg gcagcatgct gattgaccta caatccggta
     3961 acgaagatcg tggtatttgc ggcctgccgt ttacgcgtca gtccgacaat cagaccgttt
     4021 atattccgat gaatatcatt ggtaacctgt acgtctccaa cggtatgtcc gcaggtaata
     4081 cccgcaacga agcacgcgtt cagggattgt ctgaagtttt cgaacgctac gtgaaaaacc
     4141 gcattattgc tgaaagcatc agcctgccag agatcccggc agacgtgctg gcgcgttacc
     4201 cagcggtggt tgaagcgatc gaaacactgg aagcagaagg tttcccgatc ttcgcatatg
     4261 atggttccct tggcggccag tatccggtga tttgcgtggt actgttcaat cctgctaacg
     4321 gtacctgctt tgcctctttc ggtgcgcatc ctgattttgg cgtagcactg gaacgtaccg
     4381 tgaccgagct gctgcaaggt cgtggcctga aagatttgga tgtgtttact ccgccaacct
     4441 tcgatgatga agaagtcgct gaacatacca acctcgaaac gcactttatc gattccagcg
     4501 gtttaatctc ctgggacctg ttcaagcagg atgccgatta tccgtttgtg gactggaatt
     4561 tctccggcac cacggaagaa gagtttgcta cgctgatggc tatcttcaac aaagaagata
     4621 aagaagttta tattgccgat tacgagcatc tgggcgttta tgcttgccgt attatcgtgc
     4681 ctggcatgtc cgatatttat ccggctgaag atctgtggct cgcgaataac agtatgggca
     4741 gccatttacg tgaaacgatt ctttcgctac caggcagcga gtgggaaaaa gaagattacc
     4801 tgaacctcat cgagcaactg gatgaagaag gttttgatga ctttacccgc gtgcgtgagc
     4861 tgttgggtct ggcgaccggg tcggataacg gttggtacac cctgcgtatc ggtgaattaa
     4921 aagccatgct ggcgctggct ggtggcgatc tggaacaggc tctggtctgg accgaatgga
     4981 cgatggagtt taactcatca gtatttagtc cggaacgcgc caactattat cgctgcctgc
     5041 aaacgttgtt attactggca caggaagaag atcgccagcc gctgcaatat ctgaatgcgt
     5101 ttgttcgcat gtacggcgca gatgccgtgg aagccgccag tgcggcaatg agcggcgaag
     5161 cggcgtttta cggcctgcaa ccggtagata gcgatctgca cgcgtttgct gcacatcagt
     5221 cgttgttgaa ggcctacgaa aagctgcagc gcgccaaagc agcattctgg gcaaaataaa
     5281 atcaaatagc ctacgcaatg taggcttaat gattagtctg agttatatta cggggcgttt
     5341 ttttaatgcc ccgctttaca tatatttgca ttaataaaat aattgtaatt ataaggttaa
     5401 atatcggtaa tttgtattta ataaatacga tcgatattgt tactttattc gcctaatgct
     5461 cccttttaat taactatttt agcggaggat gcggaaaaaa ttcaactcat ttgttaattt
     5521 ttaaaattta tttttatttg gataatcaaa tatttactcc gtatttgcat aaaaaccatg
     5581 cgagttacgg acctataagc caggcgagat atgatctata tcaatttctc atctataatg
     5641 ctttgttagt atctcgtcgc cgacttaata aagagagagt tagtgtgaaa gctgacaacc
     5701 cttttgatct tttacttcct gctgcaatgg ccaaagtggc cgaagaggcg ggtgtctata
     5761 aagcaacgaa acatccgctt aagactttct atctggcgat taccgccggt gttttcatct
     5821 caatcgcatt cgtcttctat atcacagcaa ccactggcac aggcacaatg cccttcggca
     5881 tggcaaaact ggttggcggc atttgcttct ctctggggct gattctttgt gttgtctgcg
     5941 gagctgatct ctttacttcc accgtgttga ttgttgttgc taaggcgagt gggcgcatca
     6001 cctggggtca gttggcgaaa aactggctaa atgtctattt tggcaacctg gtcggcgcac
     6061 tgctgtttgt acttttaatg tggctttccg gcgagtatat gaccgcaaat ggtcaatggg
     6121 gactaaacgt cctacaaacc gccgaccaca aagtgcacca tacttttatt gaggccgtct
     6181 gtcttggtat cctggcaaac ctgatggtat gtctggcagt atggatgagt tattctggcc
     6241 gcagcctgat ggacaaagcg ttcattatgg tgctgccggt cgcgatgttt gttgccagcg
     6301 gttttgagca cagtatcgca aacatgttta tgatcccgat gggtattgta atccgcgact
     6361 tcgcatctcc ggaattctgg accgctgtcg gttctgcacc ggaaaatttt tctcacctga
     6421 ccgtgatgaa cttcatcact gataacctga tcccggttac gatcggtaat attatcggcg
     6481 gtggtttgtt ggttgggttg acatactggg tcatttacct gcgtgaaaac gatcaccatt
     6541 aatggttgtc gaagtacgca gtaaataaaa aatccactta agaaggtagg tgttacatgt
     6601 ccgagcttaa tgaaaagtta gccacagcct gggaaggttt taccaaaggt gactggcaga
     6661 atgaagtaaa cgtccgtgac ttcattcaga aaaactacac tccgtacgag ggtgacgagt
     6721 ccttcctagc tggcgctact gaagcgacca ccaccctgtg ggacaaagta atggaaggcg
     6781 ttaaactgga aaaccgcact cacgcgccag ttgactttga caccgctgtt gcttccacca
     6841 tcacctctca cgacgctggc tacatcaaca agcagcttga gaaaatcgtt ggtctgcaga
     6901 ctgaagctcc gctgaaacgt gctcttatcc cgttcggtgg tatcaaaatg atcgaaggtt
     6961 cctgcaaagc gtacaaccgc gaactggatc cgatgatcaa aaaaatcttc actgaatacc
     7021 gtaaaactca caaccagggc gtgttcgacg tttacactcc ggacatcctg cgttgccgta
     7081 aatctggtgt tctgaccggt ctgccagatg catatggccg tggccgtatc atcggtgact
     7141 accgtcgcgt tgcgctgtac ggtatcgact acctgatgaa agacaaactg gcacagttca
     7201 cttctctgca ggctgatctg gaaaacggcg taaacctgga acagactatc cgtctgcgcg
     7261 aagaaatcgc tgaacagcac cgcgctctgg gtcagatgaa agaaatggct gcgaaatacg
     7321 gctacgacat ctctggtccg gctaccaacg ctcaggaagc aatccagtgg acttacttcg
     7381 gctacctggc tgctgttaag tctcagaacg gtgctgcaat gtccttcggt cgtacctcca
     7441 ccttcctgga tgtgtacatc gaacgtgacc tgaaagctgg caagatcacc gaacaagaag
     7501 cgcaggaaat ggttgaccac ctggtcatga aactgcgtat ggttcgcttc ctgcgtactc
     7561 cggaatacga tgaactgttc tctggcgacc cgatctgggc aaccgaatct atcggtggta
     7621 tgggcctcga cggtcgtacc ctggttacca aaaacagctt ccgtttcctg aacaccctgt
     7681 acaccatggg tccgtctccg gaaccgaaca tgaccattct gtggtctgaa aaactgccgc
     7741 tgaacttcaa gaaattcgcc gctaaagtgt ccatcgacac ctcttctctg cagtatgaga
     7801 acgatgacct gatgcgtccg gacttcaaca acgatgacta tgctatcgct tgctgcgtaa
     7861 gcccgatgat cgttggtaaa caaatgcagt tcttcggtgc gcgtgcaaac ctggcgaaaa
     7921 ccatgctgta cgcaatcaac ggcggcgttg acgaaaaact gaaaatgcag gttggtccga
     7981 agtctgaacc gatcaaaggc gatctcctga actacgatga agtgatggag cgcatggatc
     8041 acttcatgga ctggctggct aaacagtaca tcactgcact gaacatcatc cactacatgc
     8101 acgacaagta cagctacgaa gcctctctga tggcgctgca cgaccgtgac gttatccgca
     8161 ccatggcgtg tggtatcgct ggtctgtccg ttgctgctga ctccctgtct gcaatcaaat
     8221 atgcgaaagt taaaccgatt cgtgacgaag acggtctggc tatcgacttc gaaatcgaag
     8281 gcgaataccc gcagtttggt aacaatgatc cgcgtgtaga tgacctggct gttgacctgg
     8341 tagaacgttt catgaagaaa attcagaaac tgcacaccta ccgtgacgct atcccgactc
     8401 agtctgttct gaccatcact tctaacgttg tgtatggtaa gaaaactggt aacaccccag
     8461 acggtcgtcg tgctggcgcg ccgttcggac cgggtgctaa cccgatgcac ggtcgtgacc
     8521 agaaaggtgc tgtagcgtct ctgacttccg ttgctaaact gccgtttgct tacgctaaag
     8581 atggtatctc ctacaccttc tctatcgttc cgaacgcact gggtaaagac gacgaagttc
     8641 gtaagaccaa cctggctggt ctgatggatg gttacttcca ccacgaagca tccatcgaag
     8701 gtggtcagca cctgaacgtt aacgtgatga accgtgaaat gctgctcgac gcgatggaaa
     8761 acccggaaaa atatccgcag ctgaccatcc gtgtatctgg ctacgcagta cgtttcaact
     8821 cgctgactaa agaacagcag caggacgtta ttactcgtac cttcactcaa tctatgtaat
     8881 tagatttgac tgaaatcgta cagtaaaaag cgtacaataa aggctccacg aaagtggggc
     8941 cttttttagc acgagagcct tttttgtcag ctatctatac tttaaggtga ctgccaaaac
     9001 agactcgacg tagccttcga gctgcgcacc aacacggcct cagatgggcc acatctggag
     9061 aaataccgca atgtcagtta ttggtcgcat tcactccttt gaatcctgtg gaaccgtaga
     9121 cggcccgggt attcgcttta tcaccttttt ccagggctgc ctgatgcgct gcctgtattg
     9181 tcataaccgc gacacctggg acacgcatgg cggtaaagaa gttaccgttg aagatttgat
     9241 gaaggaagtg gtgacctatc gccactttat gaacgcttcc ggcggcggcg ttaccgcatc
     9301 cggcggtgaa gcaatcctgc aagctgagtt tgttcgtgac tggttccgcg cctgcaaaaa
     9361 agaaggcatt catacctgtc tggacaccaa cggttttgtt cgtcgttacg atccggtgat
     9421 tgatgaactg ctggaagtaa ccgacctggt aatgctcgat ctcaaacaga tgaacgacga
     9481 gatccaccaa aatctggttg gagtttccaa ccaccgcacg ctggagttcg ccaaatatct
     9541 ggcgaacaaa aatgtgaagg tgtggatccg ctacgttgtt gtcccaggct ggtctgacga
     9601 tgacgattca gcgcatcgcc tcggtgaatt tacccgtgat atgggcaacg ttgagaaaat
     9661 cgaactcctc ccctaccacg agctgggcaa acacaaatgg gtggcaatgg gtgaagagta
     9721 caaactcgac ggtgttaaac cacctaagaa agagaccatg gaacgcgtga aaggcattct
     9781 tgagcagtac ggtcataagg taatgttcta atttccccgt aaagcggcaa ctttattgag
     9841 ttgccgcttt tttattccgc gct
//
LOCUS       DAJTOR010000156         9858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTOR010000156 DAJTOR010000000
VERSION     DAJTOR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.156"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..2036)
                     /gene="fdeC"
                     /locus_tag="OO249_003340"
                     /pseudo
     CDS             complement(1..2036)
                     /gene="fdeC"
                     /locus_tag="OO249_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
     gene            3152..3253
                     /locus_tag="OO249_003341"
     CDS             3152..3253
                     /locus_tag="OO249_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637522.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            3617..3880
                     /locus_tag="OO249_003342"
     CDS             3617..3880
                     /locus_tag="OO249_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR5637523.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            3880..4020
                     /gene="ykgO"
                     /locus_tag="OO249_003343"
     CDS             3880..4020
                     /gene="ykgO"
                     /locus_tag="OO249_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCR5637524.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(4055..4282)
                     /locus_tag="OO249_003344"
                     /pseudo
     CDS             complement(4055..4282)
                     /locus_tag="OO249_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5105..5647
                     /gene="ecpR"
                     /locus_tag="OO249_003345"
                     /gene_synonym="matA"
     CDS             5105..5647
                     /gene="ecpR"
                     /locus_tag="OO249_003345"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HCR5637525.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINCRFLRKDIKKDKITDQEMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            5673..6308
                     /gene="ecpA"
                     /locus_tag="OO249_003346"
                     /gene_synonym="matB"
     CDS             5673..6308
                     /gene="ecpA"
                     /locus_tag="OO249_003346"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HCR5637526.1"
                     /translation="MTHWDITSSIQLGKKCNEKKVLAIALVTVFTGMGVAQAADVTAQ
                     AVATWSATAKKDTTSNLVVTSLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKL
                     TSRLITNTLTQLDTSGSTLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYN
                     ASNRTTAQDGFTFTIISGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            6366..7034
                     /gene="ecpB"
                     /locus_tag="OO249_003347"
                     /gene_synonym="yagY"
     CDS             6366..7034
                     /gene="ecpB"
                     /locus_tag="OO249_003347"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HCR5637527.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            7060..9583
                     /gene="ecpC"
                     /locus_tag="OO249_003348"
                     /gene_synonym="yagX"
                     /pseudo
     CDS             7060..9583
                     /gene="ecpC"
                     /locus_tag="OO249_003348"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
     gene            9573..>9858
                     /locus_tag="OO249_003349"
     CDS             9573..>9858
                     /locus_tag="OO249_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637528.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLY"
BASE COUNT         2657 a         2433 c         2289 g         2479 t
ORIGIN      
        1 cagcgtttgt tgtaatgtgc ccagctgata ctgctcgctg aaagcacgca cacacgcaca
       61 acaacgtttc cggcacgggt cgaagaaacc gtagcgctgt aaacgcctgg ttttatctcc
      121 gaaattgtac caatggtgac accattagtg tcttgcggaa caaatcgcaa gcggctggcc
      181 tctcccgtca ccgggttacc atcagtatcc accgcagtaa gcgtcagcgt gtgggattgc
      241 tgaccatcag caaccacatc tcctgagagt tcattcacgc tcagagtaga cttcgacaca
      301 tccatcatcg tggcgcgcaa ttctgcagtg acggttttgc tcatgccatc aacgctaaca
      361 gtaatcgttg cctcacctga ttgcgttccg gtagtgaaga cagactgata caccccagct
      421 tcagtttcag tgaactcacc cagtgttggc tttgcctgtg atttagtggc cttcagggaa
      481 cgagtcacaa tatttccagc cggtttgaaa gttagttcag tcttgatctg atctttcatg
      541 cccgtgactg gctggccctc ggcgtcgcgc agagacagca ccagcggttt ttgctcatta
      601 ccgttagcaa gcatttgaat acggctctga ccgtcaagcg ttaacgccgt gcgatcggcg
      661 ctcataccag ctccggtaat gaccacctct gtctgcacgc gttttgaggt attgcctttg
      721 ttatcgtagg caaccgcaga aatcgcataa taattgtctt tgcctggacg ataagccggg
      781 agcgttactt gccactgact accctgaccg gtaattttgc caccttcagc cagtaatgac
      841 ggcgcttccc actgcacatt tttcagtccg tgagttgctt tgctgaccac aagcccgagg
      901 gaaagtgtct gacagccctt gccttcaata cgctcaggca gagcaatacg gatcacttca
      961 gatttgcggt actcaagaac gatgttgtta ttacgctcaa ccaggtcata gcggctgcct
     1021 gctagcattc gacgctcgcg aatgctgtct gtatcgagtt gtttctccag aggttcgcca
     1081 atccgataat taacttccag gccaaagcga gtgtcattct caccactctt gccctgctta
     1141 tgcccggcac tcagggtcag aagaggcaca ggcgtgtaat tcacttcagc ggaaatcgca
     1201 tgtggatctt tctggcgttt atctttgcca aacagcccaa cttcatcgcc ataatactgc
     1261 tcatacatca ggcttgcgcc aagctgcggt caggcgggta aatagccctc agcacgaata
     1321 tcccagccat tcgccgggcg ttcctgataa tcctcaatat ccggcgattt tttccagcca
     1381 gaagcccgaa tataaccatt ggcgctcagt ttcagataat cgcgccagta ttccgcacca
     1441 acaccaatgc gggtatgact acgggataaa tcatggtcga taaaggtgtt cacccccccc
     1501 attcagtcat ttcctgaaaa atgacgccag ccaaaaccaa tatttgactg agtacgatcg
     1561 tctgtacgat gtattgcccc ctgagtgaac aacatatttg tcggcgtatc ataaatcgga
     1621 taaagcattt ccagcgaaga atccttcagc gagaaatctt tatcgacatt cagtttgacg
     1681 cgcgctgtac catatttccc gagccactcc tgtatttcct ggttagcttt agcggtggcc
     1741 attccggtaa taaaattacg tgtcgcatcg ctatctggct gactgcttaa aaatgtcccg
     1801 gcatttgcgg caaacgacgc gacatttttc tccacgttat tatcagcagt taccgtagta
     1861 tttcccatgc tcaaccgtgg ctgaaccgca tgctgcgcac gtgccgccat tactggggta
     1921 aaggtgacag cgagtggaaa aagaacctga acagagatat ttgcccacgc cacgcagcgg
     1981 gccagaactg aataacgaaa tcgtggttgt ttatgacctg ttttataacg tgacattgag
     2041 ataatttcct gatgaacgaa ttaacgaaca taggcagaca caaataatgg cgtctgcttg
     2101 tcagaaaaac cggggatttc ccggtttcct tttccgcaga aaaggaaata cctttatgta
     2161 agaaagtgca ccgccacctg atatatccag tgtctgtagc cactcatcag gtggttgggc
     2221 gcgggattat atttttattt atgggtaaaa atttagcttt ttgtgcaagt cattaaatag
     2281 atacctaagt tatatttctt actctgcgga cagtgaataa ttcattattc agactgaata
     2341 ataatgacct gcctttaatt agcaactatt ggaaatacgt cgaagtaggt atatacgcca
     2401 ggataaaccg atatatttat tatggatatt cattttcctt catcggactg taaattgcgg
     2461 ggatatcgaa cttacactac tcatcgcaac actcaaacag atttatgaat ttcgttcata
     2521 acccctgaca aatcctgccg tctaatcgag cttttccctt tacgttatgc ttctcatgaa
     2581 cgagaacaca acttattcat gaattaacca ttccggataa actatggaat caaattcagg
     2641 catcgaaagg aactggctgg tttccgttaa tagattaaat atctattact ctcccgagag
     2701 taaaactatc aaatcaatga gtaaccgata cagcctcaca aattctttca gataattaac
     2761 ccgtcactgc ggcacgttct ctcgtaaggc gtgcctgcag tgtaatccag tatgtatccg
     2821 gcatagttga cggtttaata gaactcaatt cctctccctg gtcaccaaca acgatatctt
     2881 gtataacacc ccaggagata cgacattatg atgagatgcg atatgctcct cagcaccaat
     2941 caacgccagg actgggccag tcttgagatc agactcattc ccgggactga cttcagtcga
     3001 catatatcaa tgacgttggt gcaataaaag atagccgatg taatacatct attcaaattt
     3061 gtgatacatg tcaaaaaact ggttgaccaa actcgttatt tatataaggg cacttacgaa
     3121 gtgcgctctt ttttaaagcg aggaagtacc aatgaaagag aataaagtac aacaaatcag
     3181 tcataaacta attaatatcg ttgtttttgt cgcaattgta gaatacgcct atttatttct
     3241 ccatttctat taataacggc aataaactgt tcacttcagt gatatttaaa atatgcattc
     3301 tctccctttt tgtaagtaat tattatatcc gtgggagagg aatacacgct gtcaggtaat
     3361 caatcatact gcgataaatc atcggccagt aaagtggaga taacctccat tgtcgaaaaa
     3421 tccatactct cagcgaaacc atcatcaatc actcatccag gcgtttatgg gagcgtcgcc
     3481 aacggctgtt aacaatgcca gacttccccg ttgcggaaat tccacatacc actaatggtc
     3541 acagggattg ggtgttgaaa tgatccggat gagcatgtat ctttatggtt atgctataac
     3601 ataacaggta aaaatgatga agcccaatat ccatcctgag tatcgtactg tggtgttcca
     3661 cgacaccagt gttgatgagt actttaaaat cggctcgact atcaaaacag accgtgagat
     3721 tgagctggat ggcgtaacgt atccgtacgt gacaattgat gtctcttcta aatcgcaccc
     3781 gttctataca gggaagctga gaacagtggc atcagaagga aatgttgcac gattcaccca
     3841 acgttttggt cgttttgtta gcacgaaaaa ggggtcgtga tgaaagttct taactctctg
     3901 cgtaccgcaa aagaacgcca tccagactgt cagattgtga agcgaaaagg acggctatat
     3961 gtgatttgta aatctaatcc acgttttaag gccgttcagg gtcgtaagaa aaaacgttga
     4021 ttcaaaattc gacggattaa cgatatttgt ctgattaata atcagatcgg attaatgttg
     4081 gtgtgtttat aacaccaaca ttaattttcc tggggatata ttcttcctgt tcatttgagg
     4141 ccaactgcct gacgtttctc tacgaatatt ccattatctt aatgttgact tgttgaccag
     4201 cttcgcccct gtatgctggc atcaactctc ttttagactg aacacgccac tcagtctcct
     4261 ccctttgcgg cgcagcctgc attttcactc aaactgttaa gatgataaat gtggtaaatc
     4321 tgttggtact aacataaaaa cgtttacgcc acaggaacag tctgatccac cggtaacccc
     4381 gtcgccgacg ttcgagtgcc agttagagta acgcgcacag ataactgcac tcagtgccct
     4441 gacaaagagg ccatcgttcc tgtgacagct ggcagccttc gtttaacttc acttaatctg
     4501 gctcttgagg gcttaccgaa cagatgacgt acatacgccc gttcaatttt ccatcactta
     4561 ttgtaatgaa cacctataac cattttgtgc ggcatgttaa tccattaaaa tttcttactg
     4621 attggcaaat catcttcaat gacagctcat tatagtttta tattctatcc cttaccctta
     4681 aaacttgttt ttttactagt ccatcacaca gcgcattaag actattccta acacttcagg
     4741 gcaaagttcc tgaccaatat aaaatgcaag taagaattga acgttatatt gccaataacc
     4801 ttatgaaacc aaatgtcttt ttcttcttat caaaaaaaca atattttcag tttttctaaa
     4861 tattgactta accattgaat tccttttccg ttcacatatt gacactcatc aaaaaaaaaa
     4921 cataaattta agcccaatcg aaaataatta aacttaatct cgtttaacct ttattgatat
     4981 gtactacgta tcttatttac ttccggttta ctaaggaaac tgaatgcacc tgtaaaaatt
     5041 acaggtttgg aaagtagtga catggcaaag tgattacagt agggactatg aggttaaaaa
     5101 ccatatggaa tgtcaaaacc gttctgataa atacatctgg tctccccatg acgcctactt
     5161 ctataaagga ctatctgaac tgattgtgga tatcgacaga ttaatttatc tatcgttgga
     5221 gaaaattaga aaagatttcg tgtttatcaa tctcaatacg gattctttat ctgaatttat
     5281 aaaccgtgat aatgaatggt tatccgcggt aaaggggaaa caggtcgtat tgattgcggc
     5341 cagaaagtca gaagccttag caaattattg gtactacaac agcaatatta ggggcgtggt
     5401 atacgctgga ctgagtcgtg atattagaaa agaactggcc tatgtgatta attgcaggtt
     5461 cctgagaaaa gatattaaga aagataaaat cacggaccag gaaatggaaa ttatccgcat
     5521 gacggcccag ggaatgcaac ctaaatcgat tgccagaatt gaaaattgta gtgtgaagac
     5581 agtgtatacc catcggcgta atgctgaggc caagctgtac tcaaaaatat ataagttggt
     5641 tcagtaaact ccaggcaagt tagttttaaa aaatgactca ctgggacatc acgtcctcaa
     5701 ttcaactcgg gaagaaatgc aatgaaaaaa aggttctggc aatagctctg gtaacggtgt
     5761 ttaccggcat gggtgtggcg caggctgctg acgtaacagc tcaggctgta gcgacctggt
     5821 cggcaacagc caaaaaagac accaccagta atctggttgt gacgtcactc ggtagcttgg
     5881 cgttccagta tgccgaaggc attaaaggtt ttaactcaca gaaaggtcta tttgacgtgg
     5941 ctatcgaggg tgactcaacg gctaccgcct ttaaactgac ctcacgtctt atcaccaaca
     6001 cattaactca gttggatacc tcaggttcca cactgaatgt gggcgtggat tataacggcg
     6061 cggcagtcga aaaaactggc gataccgtga tgatcgatac cgccaacggc gtactgggcg
     6121 gcaaccttag cccgctggct aacggttaca atgccagcaa tcgtaccacc gcacaggatg
     6181 gtttcacctt caccatcatc agcggcacca ccaatggtac caccgcagta accgattaca
     6241 gcactctacc ggaaggcatc tggagcggcg acgttagcgt acagttcgac gcgacctgga
     6301 ccagttaatc tctctgatgt accagcaggg gtagcccccc tgctttattc ctggacggaa
     6361 ttgttatgaa aaagcacctt ctgcctctcg ctctgctgtt ttccggaata tctccggccc
     6421 aggcgctgga tgtcggcgat atatcatcgt ttatgaacag tgacagcagc acgctgagca
     6481 aaacgatcaa aaacagtacc gacagtggtc gccttatcaa tatccgtctc gaacggctct
     6541 cttcaccgct tgacgacggg caggttatct caatggacaa gccggatgag ttgctactca
     6601 ctcccgccag cttgctgcta cccgcccaag ccagcgaagt gatccgcttc ttctataagg
     6661 gacccgcaga tgaaaaagag cgctactacc gcattgtctg gtttgatcag gccctcagtg
     6721 atgcgcagcg cgataatgcc aaccgcagcg ctgtggccac tgcttccgcc cgcatcggca
     6781 ccattctggt cgtcgcccct cgtcaggcga actaccactt tcagtacgcc aacggctccc
     6841 tgacaaatac aggaaatgcg acgctgcgga tcctcgccta cggcccctgc ctgaaagccg
     6901 ccaacggtaa ggagtgtaaa gagaattact acctgatgcc gggcaagtcg cgtcgtttta
     6961 cccgcgtgga cactgcggat aacaaaggac gggttgcact ttggcagggt gataagttca
     7021 ttcccgtgaa atagatagct gtgcagatgg ataacgacaa tgcctttacg acggttctcc
     7081 ccaggactga aagcccagtt tgccttcggc atggtctttt tgttcgttca gcccgatgcc
     7141 agcgctgctg acataagtgc gcagcaaata ggtggggtga ttattccgca ggccttcagt
     7201 caggcgcttc aggacggcat gagcgtcccg ctctatattc atctcgccgg tagtcagggt
     7261 cgccaggacg atcagcgaat cggcagcgct tttatctggc tggatgatgg acagctacgc
     7321 atccggaaaa tacagctgga agagagtgaa gataacgcca gtgtcagcga acaaactcga
     7381 cagcagctga tggctctggc gaacgccccg ttcaatgagg cccttaccat cccctgactg
     7441 acaacgcgca gttggatctc agcttgcgcc aactgctgct gcagctggtg gtcaagcgcg
     7501 aagcgctggg caccgtacta cgctcacgta gcgaagacat cgggcagtcc agtgttaaca
     7561 ccctcagcag taatctgagc tataacttgg gcgtctataa caaccagttg cgtaacggcg
     7621 ggagcaacac atccagctat ctgtcgctga ataacgttac tgcgctgcgc gaacatcatg
     7681 tggtgctcga cggctcgctg tacgggatcg gtagcggtca acaggacagt gaattatata
     7741 aagcgatgta tgaacgcgat tttgccggtc accgatttgc cggtggaatg ctcgacacct
     7801 ggaacttgca gtcattaggg ccgatgaccg ccatttcagc agggaagatt tacggccttt
     7861 cctggggaaa ccaggccagc tccaccatct tcgacagcag ccagtcagcc acgccagtga
     7921 tcgccttttt accggcggcg ggcgaagtac atctcacccg tgatgggcgg ctactaagcg
     7981 ttcagaactt cactatgggc aatcatgaag tggatacccg gggtctaccg tacggtattt
     8041 acgatgtgga agttgaggtg atcgttaacg gtcgcgtgat cagcaaacgc actcaacggg
     8101 tcaataagct gtttagccgg ggcgcggcgt cggtgcacca ctggcgtggc aggtatgggg
     8161 cggtagcttt catatggatc gctggtcgga aaacgggaaa aagacgcgac cagctaaaga
     8221 gagttggcta gcaggtgcct cgacctccgg ctcattgagt acgcttagct gggcggcaac
     8281 gggatatgga tacgataatc aggcggtggg tgaaacccgt ctgacgctgc cgcttggggg
     8341 ggcgatcaac gttaacctgc aaaatatgct ggccagtgac agctcatgga gcagcatcgg
     8401 cagcatcagc gctactctac cgggaggctt tagttcgctg tgggttaacc aggaaaaaac
     8461 ccgtattggc aatcaattgc gacgtagcga tgccgacaac cgtgcaatcg gcggcacact
     8521 caacctgaac tcactgtggt cgaagctggg cacattcagc atcagctaca atgatgaccg
     8581 ccgttacaac agccattatt acacggcaga ttactatcaa aatgtctaca gcggtacctt
     8641 tggttcgctt ggcctgcggg ccggtattca gcgctataac aacggcgaca gcaacgccaa
     8701 tacagggaaa tatatcgctc tcgatctctc gctaccactg ggcaactggt ttagcgcagg
     8761 gatgacccat caaaacggct acaccatggc aaacctgtca gcacgcaaac agtttgatga
     8821 agggaccatt cgcactgttg gtgccaatct gtcacgtgcc atctccggcg ataccggtga
     8881 tgacaaaacc ctcagcggtg gggcgtatgc acagttcgac gctcgttacg ccagcggaac
     8941 gctgaacgtc aatagcgcgg cggacggcta cgtcaatacc aacttgaccg ccaatggcag
     9001 cgtcggctgg cagggtaaaa acattgctgc cagcgggcgg actaatggca acgctggggt
     9061 gatattcaac accgggctgg aggacgacgg tcagatcagc gccaaaatca acgggcggat
     9121 tttcccgctt aacggcaagc gtaactatct cccgctctct ccctatggaa gatatgaggt
     9181 ggagttacag aacagcaaaa actcactcga cagttacgat atcgtcagcg gccgcaaaag
     9241 tcgtctgact ctctatccag gcaatgtcgc tgtcattgag ccagaggtga agcagatggt
     9301 taccgtctcc ggtcgtatcc gtgcggaaga cggcacactg ctggctaacg cacggattaa
     9361 caaccatatc ggccgaaccc gaaccgatga aaacggcgag tttgtcatgg acgtggataa
     9421 gaaatacccc actatcgatt ttcgctacag tggcaataaa acctgcgaag tggctctgga
     9481 actcaaccag gcgcgcggtg ccgtctgggt cggtgatgtg gtctgcagcg gcctctcatc
     9541 gtgggcggcg gtgacgcaga caggagaaga gaatgagagt taacctacta ataacgatga
     9601 taattttcgc gctaatctgg ccagtaactg cgctcagagc ggcagtgagc aaaacaacct
     9661 gggcggatgc accggcacgc gagtttgtgt ttgtcgaaaa caactcagac gacaactttt
     9721 tcgtcactcc tggcggggcg ctggatccgc gcctgaccgg tgccaaccgc tggaccggtt
     9781 taaaatacac tggttcagga accatctatc agcaaagcct cggctacatt gataacggtt
     9841 acaacaccgg cctttata
//
LOCUS       DAJTOR010000157         9854 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTOR010000157 DAJTOR010000000
VERSION     DAJTOR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.157"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            6..81
                     /locus_tag="OO249_003350"
     tRNA            6..81
                     /locus_tag="OO249_003350"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO249_003351"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO249_003351"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1225)
                     /locus_tag="OO249_003352"
                     /pseudo
     CDS             complement(468..1225)
                     /locus_tag="OO249_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(1233..2336)
                     /locus_tag="OO249_003353"
     CDS             complement(1233..2336)
                     /locus_tag="OO249_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5637529.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     VLLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2346..3516)
                     /locus_tag="OO249_003354"
                     /pseudo
     CDS             complement(2346..3516)
                     /locus_tag="OO249_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3584..4645)
                     /locus_tag="OO249_003355"
     CDS             complement(3584..4645)
                     /locus_tag="OO249_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5637530.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LLGFSYADADGKFSGIDVDVDVDVDVDVDVDVCRSVAAAVFGDDTKVKYTPLTAKERF
                     TALQSGEVDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGAT
                     VCIQAGTDTELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALR
                     IKLSNPAEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDE
                     KAANPATPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIK
                     RGQNNLWNNGGIQYAPPVR"
     gene            complement(5076..5297)
                     /locus_tag="OO249_003356"
     CDS             complement(5076..5297)
                     /locus_tag="OO249_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637531.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGCLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIAMSK"
     gene            complement(5550..8654)
                     /gene="acrF"
                     /locus_tag="OO249_003357"
     CDS             complement(5550..8654)
                     /gene="acrF"
                     /locus_tag="OO249_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCR5637532.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTNDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALSV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFLPVFFVVIRR
                     CFKG"
     gene            complement(8666..9823)
                     /gene="acrE"
                     /locus_tag="OO249_003358"
     CDS             complement(8666..9823)
                     /gene="acrE"
                     /locus_tag="OO249_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR5637533.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYNSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     GTVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2610 a         2553 c         2365 g         2326 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcaaattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtcttcc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggcccagt cttccgactg agccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc tcgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcaaaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg taaaaaattc atcaggtgca
      541 gcttgttcca ctatctcccc acgatccata aaaattaccc ggtcagcgac ggtacgtgca
      601 aaccccatct catgtgttac acacaacatt gtcatacccg actgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctccgga tcgaggccga cgttggttca tcaaacaaca
      721 taattttcgg cttcatacac agcgaacgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttcttaggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtaa aacggtcaga tgagggaaga gattgaaatg ctgaaatacc attcccactt
      961 cttgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctacgacaa
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aaccagaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat ggacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgttggct ataacgtgac atgctgaaac aaaagatcca gtagatcagc
     1321 gcggcgaaaa catacccttc cgtcgacata cccaaccagg cgggatcaac ggttgcctgc
     1381 tgaacgctac tgaaaagatc gaacaagccg atgatgatca ccagactggt atctttgaag
     1441 agtgcgatga tggtatttac cagcccagga attaccagct tcaacgcctg tggcagaata
     1501 accagcccct gagttttcca gtaccccaac gccagcgact ctgccgcttc atattgccct
     1561 ttaggtaggg cctgtaatcc gcctcgcacg acttccgcaa catatgctga ctgaaacagg
     1621 atcacgccaa ccagcgcgcg gatcagtttg tcgatactgg tgccttctgc cataaacaac
     1681 ggcagcatga ccgaagacat aaacaatacg gtaatcagcg gtacgccgcg ccagaactcg
     1741 ataaaaatga ccgataagat acgcacaatc ggcatatggg agcggcgacc taacgccagt
     1801 aagatccccc acggtagcgc ccccgcaatc ccaacggatg caataattaa agttagcgtc
     1861 agcccgcccc attgccgggt ttcaacccgc tcaagaccaa aaaaaccgcc atacatcagc
     1921 caccagacaa tcagtgggta aatcaccgcc cagaccgcaa tatagcgacc gcgatgcggg
     1981 agtattttcc agaacattgg tgcgattgac acaaggccaa tcagtaatac taggttaatt
     2041 cgccagcgtt ggtcgtgtgg gtaaagtcca tacataaact gaccaaatcg ttcgtggatg
     2101 aagacccaac aggcaccggc ttttgtgcag tcggcacgcg ttgagccaac ccagttagcc
     2161 tgcaaaaatg cccagttcag caacggtggg atcagttccc acatcaacca tatgcagcca
     2221 atagtcagca ggctattgct ccagctggag aacagatttt ttcgcaccca caccatcgcg
     2281 cggcttgagt tatggctcgc tgggcgcgag ggctgagaca gcaatacttt tgtcatcatg
     2341 actccttagc gttcaacgat cgcgatgcgg cggttataga tattcatcag cagcgagata
     2401 gtcaggctga taatcagata gaccgacatg gtcatggcta tcgtctcgat ggcttgcccc
     2461 gtctggttca gcacggtgcc agcaaacagc gaaaccatat cgggatagcc aatagcggcg
     2521 gcaagagagg agtttttgac gatgttgaga tactggctgg ttaacggcgg aataatcact
     2581 cgcagtgcct ggggaataat gacctggcgt agcgtaaccc cagtgaccga gccgcttcat
     2641 gttgaccata aggcactgcc tggatcccag cgcggataat ctcggcgata aatgcagagg
     2701 tataaaccga aagtgccagc gttaaggctg ccagttcagg aattaaaacc attccgccgc
     2761 ggaagttaaa gcctcgtagg gctgggacat cccagtgtag tgctgcgcca aaaagccatt
     2821 gcgccagtaa aggcaaaccg atgatcaaca ctgcggcgaa cggccaggtg cggcgcagtt
     2881 gtccggtctt tatctggtat gttttattaa agcggaatag cccaacagaa aggactatag
     2941 ccataacaac agctaggata aacgcaataa atccgtctcc cagctgcggt gacggaatat
     3001 aaagcccacg attgctcaaa aaggccagat caaacgcgct cacagcctgg cgcgtggtcc
     3061 gggcaaattg cgcaacacgg caaagtacca gaagaagatt tgcagcagcg ggggaatatt
     3121 acggaagatc tcaatataaa ttgtggatag ctttcgtagc agtcagttat ccgaaagtct
     3181 cgtcagaccg ataaagaagc ctaaaacaga agcgaacaca atacacaatg cagaaaccag
     3241 tagcgtattg agtaagccga caataaaaac gcgtccgtag gtgtcgccct gctggtaatc
     3301 gatcaaatgc tggacaatac cgaagccagc gccgcgatcc agaaaggcaa aacctgaagt
     3361 aatgccacga ttactgagat tcgttacagt gttgtgaaat aaccaaccaa caattccgac
     3421 aacagcaaca acggcaagga tctggaataa ccaggcacga accgtagggt tggcaaagga
     3481 gagtgagcct ttaacggttg agcggcgatg agacatagaa acctcggaaa gcataacgct
     3541 gaagagagcg ccgccgtagc gacgctcgtt acctcacacc gacttaacgc acgggcggcg
     3601 cgtactgaat accgccgtta ttccagagat tattttgccc acgtttaatt ttcagcgggc
     3661 tttctgaacc tacgttacgc tcgaaaattt ccgagtagtt acccacctgt ttgatgatgt
     3721 tataggccca tttattatcc aacttcagat ccttgccgta atcgccttct ttacccagca
     3781 gatgtgccat atcaggcgtt gctggattag ccgctttttc atcgacgttc tgggaattga
     3841 tgcccatctc ttcagcattc agcatggcga aaagcgtcca gcgtacaatc gagaaccatt
     3901 catcatcgcc acgacgaact accggcccaa gcggttcttt agagataacc tccggtaaga
     3961 caatccattc agctgggttg cttaatttga tgcgcagggc atacagttgc gactgatccg
     4021 aggccagcgt atcgcagcga ccagattcca gtgccttcgc tgattcgtca gagcgatcga
     4081 aagtcaccgg tgtgtacttc atattgtttg ccttgaaata gtcggcaacg ttgagttcgg
     4141 tatcagtacc cgcctgaata cagacggtag cgccatccag ttctttcgcg ctttttagcc
     4201 ccgctttatc gtgcgtcagg aagccaatgc cgtcgtaata agtgacgcca gtaaatgcca
     4261 ttcccatccc ggcgtcgcga gatgaagtcc aggtcgtatt acgggagagc aaatccactt
     4321 cccctgactg taaagccgtg aagcgttctt ttgctgtgag tggggtatat ttcactttcg
     4381 tgtcgtcacc aaatacagca gcggcaacac tacgacaaac atcaacatca acatcaacat
     4441 caacatcaac atcaacatca acatcaatac ctgaaaactt accgtctgca tcggcataag
     4501 agaacccaag taatccatca ctgatcccgc attgcacaaa accttttttc tgaacggcat
     4561 caagcgtcgc gccagcatgc gcctgatttg caacggcaag cagcacgctg gcggcagcca
     4621 gtgtggctat catcatcttt ttcataatgc ttcctgtgag gcgaaaatta tcgttatagg
     4681 acgtttggga aacgtctttt acctgtctgt ggccatcctc ttataagcaa aagtagtgcc
     4741 agcttttgcg cacgatttcg cgtcgcaata agctgagtgt agacaggaaa taataaaaac
     4801 agcgccccgc aatggtgcga aaatgaaaca tgcgccacat tttggcgcaa aatatttacg
     4861 cgtgttttac taagcagaat aatgattgtt agcgtgagta tatttctggt gggaagtgtg
     4921 aaagttaaat tgatgcgtta tttcgaaagc agcgaaataa cgcgatataa catgctaaat
     4981 cagcagagaa ttagccagca gaatatagca atattttatc ttatgtaaag atactgtatc
     5041 gttaaacaga gcgcgaaact ccgcgccctg ttcagttatt tcgacatagc gatgatcccc
     5101 atcgtcaggc cagcaacagc cgctgccccg ccggcaatag ccccagccgt ttcccagtta
     5161 tcctgagtcg tgcctttcat acaggtattg gtgtgaacca gacaaccctg atcgtcataa
     5221 accggaacgc agggcgaatc gtgagcacag cccgccagca atgcggtgag caatgccaca
     5281 ggaattaatc ttttcatggt gttacctcag taataaacgc taacgcttaa taagttaact
     5341 ctcttattaa cgagctgaat gccattttat caccggaggt tataaatatt gcggaatgaa
     5401 taatgacaaa ttatgtgaat tgtaaaaata gtgaagggag cgggaactat agaaagtttt
     5461 taaagagtga gtggaagaat atcttattaa tttttatggg tttcactgaa aataataaag
     5521 gcacccgaaa gtgcctttat gtttctgatt tatcctttaa agcaacggcg gatcaccaca
     5581 aagaacaccg gtaggaagaa gattgccagc aacgttgcag agaccattcc gcccattacc
     5641 ccgataccca ctgcgttctg cgcgccactg cctgcaccgt tactgatagc tagcggtaat
     5701 acgccgagaa taaaggcgag agaggtcatc aggataggac gcagacgcat acgtactgcc
     5761 attagtgtcg cttcaacaac acctttaccc tctttctcca tgagatcttt agcgaactca
     5821 acaatcaaaa tagcgttttt ggccgacaag ccaattgtcg ttagcaagcc caccataaag
     5881 tagacgtcat ttttttgatt aaagagtgtc gccgccagca gcacgccgac aatccctaac
     5941 ggcacgacca acataaccga gacaggaatt gaccagcttt catagagtgc agcaaggcac
     6001 aggaaaacaa ccacaaagga aattgctact agagcgggag cctggtttcc cgataagcgt
     6061 tcctgatacg acatacccgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
     6121 ttttccatca acgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
     6181 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
     6241 gtggtaaagg ccgagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
     6301 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
     6361 ccacggtcga tgaagtcgtt aacgtaagtc ccacccaacg ccgttgaaat ggtctgattg
     6421 atgtcagaaa gtgaaacact taatgcctgc gccttttcct ggtcaacttc cagtttaaac
     6481 tgcgcggtgt cttccaggcc attaggacgc acgctgacta agctggcagg atgttgcgcc
     6541 gccataccaa gcaactggtt acgggcctgg gttagggcat cgtgacccag cccagcctga
     6601 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
     6661 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
     6721 gcttccgcac tgttttcatc accattacgc tcttcccacg gtttcagact gacgaaggcc
     6781 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttca
     6841 acgttcgctt tctcgttctt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
     6901 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
     6961 tcaggtaaga aggaagacgg aagacgtaaa aacaacacca ccattcctgc aacaatcagc
     7021 gcatagatca gtaaatatcg tcctgtggaa ccgaggattt tgccgacgct gttggtgtag
     7081 tggttaacgc tatgatcgaa ggtggtatta aaccagccga agaaaccgcc cttattttcg
     7141 tgatgctcag cagagacggg tttaagcagc gttgcacata acgcaggggt aagaatcaat
     7201 gccaccagaa cagaaagtgc cattgccgaa acgatggtga tagagaactg gcgataaatt
     7261 gccccagtag aaccgccgaa gaatgccatc ggaataaata ccgctgacag caccatcgcg
     7321 atacccacca gtgcgccctg aatttgcgac atcgattttt ccgtcgcttc ttttggcggg
     7381 agcttatcct ccatcatcac gcgctcgacg ttctccacca ccactatcgc atcatcgacg
     7441 agcagcccga tggcaagcac catcccgaac atcgttagtg tgttgatgga gtaaccaaaa
     7501 gcggcgagga tggcaaacgt ccctaacaac accacgggta ccgcaatggt ggggatcagc
     7561 gttgctcgca tattctgcaa gaacagatac atcaccagga acaccagcat aatggcttcg
     7621 aacagcgttt ttaccacttc gtgaatagaa agctggacga atggcgtggt gtcataaggg
     7681 tagagaacct tcattccctg cgggaagaat ggctgtaatt ccgccagttt tgccttaatg
     7741 gctttcgcgg tatcgagagc attcgcgccg gttgccagct taatccccag gcccgccgcc
     7801 ggttttccgt tgatacgagc gataacgtta tagttttcac cgccaagttc aacccgtgcg
     7861 acatctttca ggcgtaccac cgagccgtca ctgtttacgc gcagggtcac tttgccgaat
     7921 tcttccggat tttttaaccg cgtttgagca ataatcgagg cattcaactg ttgccctggt
     7981 aacgctggcg ttccgcccaa ctgtccggca gcgatctgat cgttctgtac cttcaactgg
     8041 ttaatcacat caaccggtgt cagtttatat ttgtttagca gatcggcgtc cagccagata
     8101 cgcatcgcat actgtgcgcc gaaaagctgt acgtcaccga cgccattcag acggctaagc
     8161 gtatctttaa cgttagaggc cacatagtcc gagatatcgt cctgtgtggt gcctgggtta
     8221 tcagagacaa aacccgccac catcaaatag ctgctactgg acttttcaac actgatcccc
     8281 tgctgctgaa cctcctgcgg cagcaacggc gtggcgagct ggagtttgtt ctgcacctgc
     8341 acttgcgcga tatcaggatc ggtcccggac tggaaggtaa gggtaattgt cacgctaccg
     8401 gcggaatcgt tggtggagga catatacatc aggttatcga taccattcat attctgttcg
     8461 ataacctgcg tcaccgtatc ctgcacggtc tgcgcatcag cacctggata gtttgccgaa
     8521 acagaaaccg caggcggtgc aatggttgga tactgagcga cgggcaattg taggatcgcc
     8581 agtgcgcccg ccatcatcag aataatggcc agcacccatg caaatatcgg tcgtcgaata
     8641 aaaaagtttg ccatgtcaga ttaccttact tcgatgcagt atctgcgggg gtatcggtag
     8701 tggctttaac ctgctctccc ggacgcgctt tttgcaggcc gctgacaatg acttgatcgc
     8761 cagatttcag accttcgcta atcaaccatt tatcgccaat cgcctgactg gcaacgacag
     8821 ggcgcgcttc cacctgactt ttatcgttaa caatcagcac ggttgcatca ccacgcggtg
     8881 tgcggctaac gccttgttgc gggataagaa tggcgtcagg ttggacgcct tcatcaatcc
     8941 gtgcacgcac atacataccc ggcaaaagcg tatgttgcgg gttagggaag acagcacgta
     9001 gggttatgga gccggtgctt tcatcaacgg tcacatcaga gaattgcagc gtacctttca
     9061 ggggataggt ttgaccgttt tccatgacca actctacgtt gctggtggcg ttttccttat
     9121 gcaaatttcc ttgctctacg gattgcttca gcctcataaa atcgttgctg gattgggtca
     9181 catcaacgta gataggatcg agctgctgga cagtcgccag ttcagtcgtt tgcccattag
     9241 tgacaagagc gccttcggtc acggtcgatt tgccgatacg tccgctaatt ggcgcagtca
     9301 ctttggtata agcgagattg atgcgagcgc tttcgactgt gccttttgcg gcaatcacgg
     9361 cggcatcggc ctggcgagca tcagcaatgg cctggtcgta ctcctgctgg ctgatgtatt
     9421 tcgtacccac gagcggaacg taacgtttta ccgtcaaatg cgcgatggcg gcggcggctt
     9481 cacttttcgc cagttcgcct ttcgcgctgt tataatttgc ctgataggtc gcgggatcga
     9541 tctggtacag ggactggcct gcttgtacat cgctgccttc agtgaaatta cgattcagta
     9601 cgatcccgct aacctgtggg cgaacttcgg ctatacggta agcattggtg cggcctggta
     9661 attcagtctt aacttctaac ggggccgttt tcacaatatg aacggtaacc tgcggttcac
     9721 cgacgtgagc tttctcttct cccttatcgt tacaaccggc gattaaagcc gcggagatca
     9781 gaataaagga gggcaggagg aaaaacctgg catgtttcgt cattactatt cctcaaaaaa
     9841 ccaaaagcgc gtta
//
LOCUS       DAJTOR010000158         9758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTOR010000158 DAJTOR010000000
VERSION     DAJTOR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.158"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(190..531)
                     /locus_tag="OO249_003359"
     CDS             complement(190..531)
                     /locus_tag="OO249_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5637534.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDRDK"
     gene            complement(534..4304)
                     /gene="tamB"
                     /locus_tag="OO249_003360"
     CDS             complement(534..4304)
                     /gene="tamB"
                     /locus_tag="OO249_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5637535.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAQEEVVEPKIENPQPDEKPLGETLKDLFSRPVL
                     PEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDSNQG
                     IVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGPVDM
                     DLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMRTAV
                     KGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTLNGI
                     NTAKEFPEWPSKLNGLIKTSGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGNSYM
                     QWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKVRGT
                     VEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNINLV
                     TLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPWSLT
                     RDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLKPFM
                     PETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTLNLT
                     AELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIFTRG
                     EKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMRSTL
                     AGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFEATP
                     NLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNLIVH
                     VGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGELLF
                     SGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSYLLR
                     GQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQVVVS
                     GYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(4301..6034)
                     /gene="tamA"
                     /locus_tag="OO249_003361"
     CDS             complement(4301..6034)
                     /gene="tamA"
                     /locus_tag="OO249_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5637536.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSCGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6240..6878
                     /gene="msrA"
                     /locus_tag="OO249_003362"
     CDS             6240..6878
                     /gene="msrA"
                     /locus_tag="OO249_003362"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5637537.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNEPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            7201..8544
                     /locus_tag="OO249_003363"
     CDS             7201..8544
                     /locus_tag="OO249_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5637538.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(8606..8812)
                     /locus_tag="OO249_003364"
     CDS             complement(8606..8812)
                     /locus_tag="OO249_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5637539.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            9137..9691
                     /locus_tag="OO249_003365"
     CDS             9137..9691
                     /locus_tag="OO249_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5637540.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
BASE COUNT         2256 a         2671 c         2402 g         2429 t
ORIGIN      
        1 catactcacc gttcaaagcg tgcccccgtt ttacccctgc acatttgtga acgccagcag
       61 agttttttat gcgattgcag atatggaaag cggctcggag agaaaagcaa aagatggaaa
      121 agttacaaaa aaaaatcgcg agatacatcg cataaaacaa cgccacccga aggtggcgtg
      181 cttttattat tacttatccc tgtctaacca gtcgccgctt tcaattagct ttaatccctc
      241 gacgggtcgt tgataaacgt acatccatgc actcccatac ggcgtctgaa tcaactggcg
      301 cgcgtattca ccgcccctgg tgcgcaaggc atcaagttcg gccagcgtgg cgttgtcaat
      361 acgataaact tcaccgtgta ccgttccgtt ccccggaact gcgcctggat agtggcccag
      421 gctatacaac tggtagttat cgatactgaa atcgcccagt aactgggcat tggtcatcca
      481 gtgactgttg ccttgtttgt ggcgtaaact gccgtagaca aatattcgca ttgctaaaac
      541 tcgaactgat agagcaaatc cagtgcctgg tctacaccag acacggcttc cagatatagc
      601 ttaggcatca ggcgataacg taacgtgagt gttgctatag agtcaaatat acccacgccg
      661 tatttcactt gcaggcctgg caatacatag ccgctgacca ctacctggga ggagtcgcct
      721 actccctggg tgtcgagcgc taaattgctt acgccaaacg tctcgccgat tttacccaca
      781 atctggccac tttgcgcaac ccccagacca atcagcatcg aggtcattgc cgcactgtcg
      841 ctctgatcgc tctccagccc ttgtccacgt agcaaataag acaatgcagc ttgttgcgac
      901 atcgccgggt cagagaagat ctccgctttc ggttcgtccg ccagaccagt gacgcgaact
      961 ccggcgatta cgtcgtcttc tgtagcatcc gggttacgaa tagcttcaat attaagatac
     1021 ggttgatctg gcggaccaga gaacagtagc tcacccttac gcacaatcag atcctgacca
     1081 taggcatgga agcgcccttc agggatgttg atctgcccgt tcaggcccag cccttgtttg
     1141 tcctgaacga cgttaagatc gcccgtcagc cgcgctttca ggccaaaggc gtcaatgcgc
     1201 acattgttgc caacgtggac aatcaggtta ctgttaatcg gaatcgacgc cgttttcggc
     1261 tcttccggtt gcaggttatc gttaagcatc accacatcgc tggagacgcc tactgcgctt
     1321 tccggcagat cgtgcaccac gatgcgcgcc cacgggacat ccacgcgacc atcgagggta
     1381 aacaggtttg gtgtagcctc gaatacaaca tctggcgata catccattcg taccatcggc
     1441 ggcacggtga tccgcacttt actgcctttc gccgttactc gtgcccgcca gttttcaatt
     1501 tggctccagt cggcatcacc gttcaggtag atttcaccct gctgggtccg tactgtaccg
     1561 gcaagcgtcg agcgcatacc gttaaagttg accgcaagct ggctcggctg catatcaaac
     1621 ggcataaagt tgccgtcgat atccacaccc gtaacctgaa gctgaccaaa caactgcggg
     1681 ctttgcacat caccacccag acgcaagttg gcactcacca tccccgctgc tttttcccca
     1741 cgagtaaaga tggggtttat catcgcaagg ttgaagttac ggatattgac gttgccacca
     1801 agattacggc ggccttgcgg atcggtcacc agcacctgtc catcaaactg gccgttattg
     1861 gtcaggcgga tggtccagcc caattcggca cggttgttac gcaattccgc cgtcagattc
     1921 agtgtctgaa acgccaccgg cagcgccgca tcgttgacgg tttgcgttac ctgcacgtta
     1981 cgccccgaaa gggtgatact gccctgcggc agcccctctt tcgtggtgtc ccaggcaaca
     2041 tccgctttac ccgtgaagat accgctggcc tgagtggttt ctggcataaa cggtttcagc
     2101 atggcgaagt cgaagcggtt gagattcacc accgcacgcc cttcggcccc cgcatcgata
     2161 gtttgcggca cgcacagttc cgcattcggg ttaagccagc agtgtggccc gatgctgatt
     2221 ttttgctcct tattgcggta atccagcgca atatcgcggg tcagcgacca cgggccgacc
     2281 agcgtctgga agcgggtatt gctaagagtt cccttccagc gttcttcttt gcgatcaaaa
     2341 cttcctgcca gattaagctg cccggagaca ggctcgccct gaatccgcaa ctgtagctcg
     2401 tgctgctttt cgctgccttt ggcattcagg gtgacgaggt tgatatttac atccggctgc
     2461 gaaatttgct caacgcgtac gtcgagtttc ccggcgatct gatcggtgga tttaatgtcg
     2521 ccttccacgc gaacctgcgt cacggaaagt tcctgccagc gcaggccgcg cgcggtgata
     2581 tctgccagta gttgtggcgc ttccaccgtg ccgcgtactt tcaccagccc tttcgctgta
     2641 ccgccaagcc ccggcagcgc gttatccagc cccggcgcgt tgatggtggc atcaagattg
     2701 agatctttta cccccagctc gcctttcact tcggcactgt ttggccccag ttccagatga
     2761 agcccaggga tcatccactg catataactg ttgcctttca gcgtgccgtc aacgttcact
     2821 ttgttctgtt tgacgttacc ggtcagcttc agttctggca cctccatctg ccaggtgccg
     2881 ccgtacaggc taccgctggt tttaatcaag ccattgagtt tcgacggcca ctccgggaac
     2941 tctttggcgg tgttaatgcc attaagcgtt agctcaccgc gccaactaat ggcctgctgc
     3001 cagtcgagca acgccttgag ttcagttttc ccttccagcg ccgcgacggt gagtttgtcg
     3061 agattgacct gctgttcatt acctttggca tcaagggtaa tggtggctgg cgggatctcc
     3121 tgtcccttca ctgccgtacg catagagagc gtgtaatcgg tcattttgcc ggtaagtttc
     3181 agtttcagat catccgcctg atactgcttc tcaccagtga acggccagta aagctgtttg
     3241 ctgttcactt ccacgttgag cggcaatccg gcttccgcca gtcgcgtctg ggcgcgtaaa
     3301 tccatatcca ccggaccgga aagattaacg ccaatctcca gctgttcgcg cagcgcgccg
     3361 cccactttta gcttcacttt ttcacctttc aacggctcca cgttcagtgt gctgttgagg
     3421 gtgatatcca ccggccagtt gtctgacagc tgcgccgtgc cgctggcgtt gacgatcccc
     3481 tggttggaat cgatatccag ggcgtccagt ttagtattgc cgtcaatgct gctcactttc
     3541 agcagcatgg tgcgcacggt gatgtccgtg tcgcccgtca cgcgtaactg ctcgccttta
     3601 aactcttcaa tgttcaggtt aagcggcaaa tgcaagtcgg tcatttccgg caataccggg
     3661 cgagaaaaga gatctttcag cgtttcgccg agcggctttt catccggctg cggattttca
     3721 attttcggtt cgaccacttc ttcctgcgcc actttcggca gagcaatcag caggcctttc
     3781 agcgacgtcg gtttcagggt cagggttttc tcctgccagt tcaggccgga ggtgaagtcc
     3841 atcaccgaca ccgtggtgtc atcaatcttg atgttgacgt tgtccagcgc cacccgtgtc
     3901 agggtgatgg gatacggcgt ggagagatcc agcggaccgc tatcttcctc ttcttcaacc
     3961 tgttcagaag gaggcatttt tttactgtcg atgttgacct gaatgtcttt cagcgccagg
     4021 tcattaatac aaacgctact gttccacagg cactcaagac cgaccgccag atgcagatta
     4081 cccgctttta ccgccacgcc tggctgctca taacgaacgt cagacaaggt gagatcgcgc
     4141 cagccgccgg tgaccttgcc aatatccagt cctggcaccc agcgatccgc cgctttaaat
     4201 acaagatgca ggccgctggt ggtgcccacc agaaacgcca ccgatcccag caacagtaag
     4261 ataacgataa ccacgccgag gctgattttt ttccataaac tcataattct ggccccagac
     4321 cgatgtaaaa ctgtaacccg tgttcgtctt tatccgcgac cggtacggca aaatcgagtt
     4381 tgattggccc gaccggcgat tcccagcgca cgccgacccc ggtaccggtt ttaaagtcgc
     4441 tgcggcgaat atcgcttacc gcttcgccac tatcgacaaa caccgcgccc caccattttc
     4501 cggtcacgtt gtactggtat tccagcgatc cggttatcaa cttcgaggcc cctttcaggt
     4561 caccgttggc gtatttcggg gcgatagatt tgtatttgta gccgcgaata ctgcgatcgc
     4621 ccccggcgaa aaaacgcaga tccggcggta ctttgtcgaa atcaccggtt tcaatccagc
     4681 ccagcgtgcc gcgtgtaacg aaacgatggc gatcgtacag tgtgcggatc cagacgttct
     4741 gcgcctggaa aacggagaaa tcgacatctg agccccaggc cgtgttggag tagtcgatag
     4801 agtagcgttg cgagtcgccc caggttggca tcaggccacc acaagaacgc gtgcggctaa
     4861 tcatcacccc aggataaaac agcatcgtgg tgttggtaat ttcaccctga gtaaagtggt
     4921 cgagactcca gcgcaggtta atggcacgct gccaaccgct ggagagatcc cagtagcgag
     4981 aagccaccag cgtagtggag tcagattcgg tatcgttcag gtcagtgcgc ttaaaaccgc
     5041 cctgcaccaa ataatattgt tccagtggat tcttcagcag cggcatttta tagctgaagt
     5101 cgagggtctg ttccggcgcg gaaatactgg tactggtggt caaactgtga ccgtatgagt
     5161 tcatccacgg ctttttccac gtcgctttca cgcgcggtcc cacgtccgta gagtaaccga
     5221 ccccggtttc gatggtgttt tctgttcgcg gcgaaaccac gcccgtcaat ggtaatactt
     5281 tcgtttcgcg cgctttatca aattgtggag ccaccaccac cgagttaaac cagccggtag
     5341 cagaaagtcg gcggttcagt tccgccagat ctttcgattc atactcatcg ccctctttaa
     5401 acggcaccag attttgcagg tattcatcgc ggatttgtga tccttcaaag gtcacatgcc
     5461 caaagcggta acgttcgcca ctgttataat caatatccca gaaggcttta tgcaggccga
     5521 gcgcaatgcc cagctgcgct ttggtaaatt cgctatcgaa ataaccttta cgcaacgcaa
     5581 tactggttaa ggactttttg aaattttcat aatcgccctg gttcagtacc gtaccaatag
     5641 ccgggcgagt atcgagcaat ttcaaatagt ctttatcggt ccgcgcgccg ccgcgcaata
     5701 ccacatcggt gccgccaatt aacaccggca cacctggcgt gactttggcg atcaatacct
     5761 gccgcccttt ctttggcggt ggacggagat caaattcaat ggtcggctgg taataaccca
     5821 gcgctttcag accttcacgg atggcatcat cgacgcgtgc gcgaaagcgg cggtctggcg
     5881 tcacttcatc actttcaatc gtagaaagct gcgcacgaac gttcttttcc agctgtcccg
     5941 ataacccctc gacctgtaga cggacgttcg cggcgacggc agatccgctt aagcagagta
     6001 agcttacaca gcataactgt cggatatagc gcacattttc tcctgaatat ccttttttcc
     6061 tgcccctgga acgccgttaa acggcttaac aaaaatccag taatatggat taaaaagcag
     6121 actaaacccc aaatatttct tatgttttac tttagaccta ttcacggtgg ttattgtgtg
     6181 caaatacgcc tcttgttaca accttaaccc caatgaccga ttttcgggag agcgacacca
     6241 tgagtttatt tgataaaaag catctggttt cccccgccga tgccctgcct ggacgtaaca
     6301 ccccgatgcc cgtagccacg ctgcatgcgg tcaacggtca ctcaatgacc aatgaacctg
     6361 acggaatgga gattgccatt tttgcgatgg gttgtttctg gggtgtggag cgtctgttct
     6421 ggcagttacc cggcgtttac agcaccgccg caggctatac cggcggctat acgccaaatc
     6481 cgacttatcg ggaagtgtgc tccggtgata cgggtcatgc cgaagcggta cgcattgttt
     6541 acgatccttc cgtcatcagc tatgagcagt tgctacaggt attttgggag aatcacgatc
     6601 ccgcccaggg aatgcgtcag ggcaatgacc acggcacgca gtatcgttca gcgatttatc
     6661 cgctgacccc agaacaggat gccgcagctc gcgccagtct ggaacgtttt caggcggcga
     6721 tgcttgccgc cgatgacgat cgtcacatca ccacggaaat cgctaacgcc acaccgtttt
     6781 attatgccga agatgaccac cagcaatatc tgcataaaaa cccatatggt tactgtggaa
     6841 ttggcggaat tggcgtctgt ctgccgccgg aagcatagcg ttacgggtac aaatgtagtt
     6901 tgttgataaa gtgctctttg tctatgcctg atgcggctta aacgacttac caggcctaca
     6961 aaatcgtcca aattcaatat attgcaagga ctgcgtaggc ctgatcggca tagcgcatca
     7021 ggcagtattg catttatcag cagtctgaat tttaaccctc tggcgacttt acagtacctt
     7081 acgctatact agccactgaa aattccggtt cactttcttc gaatcggctt tcaatgtgta
     7141 tttcacacaa attaatcaac tttcccttcc gaggatctgg cctgaaaggt cggataagat
     7201 atgttaaaca gtattttagt catactctgc ttgatcgctg taagtgcgtt cttctcgatg
     7261 tccgagatct cgcttgccgc ctcacgcaaa atcaaactta aactgctggc tgatgaaggc
     7321 aatataaatg cccaacgcgt tctgaatatg caggaaaatc ccggcatgtt ctttaccgtg
     7381 gtccaaatcg gtctgaacgc agtggcgatt ctcggcggta tcgtcggtga tgcggcattt
     7441 tctccagctt ttcacagcct gttctcccgc tatatgtcgg cagagctctc tgagcaactg
     7501 agctttattc tctctttctc gttagtgact ggcatgttta tcctgtttgc ggatttaacc
     7561 ccgaaacgca tcggtatgat tgcgccagaa gcggtggctt tgcgtatcat caacccgatg
     7621 cgcttctgcc tgtacgtttg caccccgctg gtgtggttct tcaacggcct ggcgaacatg
     7681 atcttccgta ttttcaaact gccaatggta cgtaaagatg acatcacttc tgatgacatc
     7741 tacgcggtag tggaagccgg tgcgctggcg ggcgtgttac gtaaacagga acacgagctg
     7801 attgaaaacg tctttgagct ggaatcccgt accgttccgt cttcaatgac accgcgtgaa
     7861 aacgtgattt ggtttgatct ccacgaagat gagcaaagcc tgaagaataa ggtggcggaa
     7921 catccgcact ctaagttcct cgtctgtaat gaagatattg accacatcat cggttatgtc
     7981 gattctaaag acctgctgaa ccgcgtgctg gctaaccaaa gcctggcact gaacagcggc
     8041 gtacaaattc gcaacacgct gattgtgccg gatacgttaa ccctttcaga ggcgttggaa
     8101 agttttaaaa ccgcaggtga agacttcgcg gtgatcatga acgagtacgc gctggtggtg
     8161 gggatcatca ccctcaacga cgtgatgacc acgctgatgg gcgatctggt cggtcagggg
     8221 ctggaagagc agattgtcgc ccgtgatgag aactcatggc tgattgacgg cggcacccca
     8281 attgacgacg tcatgcgcgt gctggatatt gacgagttcc cgcagtcggg caactacgaa
     8341 accatcggcg gctttatgat gtttatgctg cgtaagatcc cgaaacgcac cgattcggtg
     8401 aaattcgccg gctacaaatt tgaagtggtg gatatcgata actaccgcat cgaccagctg
     8461 ctggtgaccc ggatcgacag caaggccacc gccctttcgc caaaactgcc tgacgctaaa
     8521 gataaagaag aaagcgtcgc gtaaccccca gaaacatcaa cggctcctga atcaggagcc
     8581 gtcttattac tgcatagcac tttggttaag ccatctctgt ttgcagacgc ataacctgac
     8641 ggttaacttc ggacatcact gacaaatgca gcttatcctt cacttttggg ataagaatct
     8701 tacccttatc aaattcaaaa gcgccaacgt ccttgatgta taaccgtcca cggaacagga
     8761 tctttacgta cttcgccacc tgaagtgggt tgtaccgttg gaaaattttc attgttatct
     8821 cctgctgagt attacgcctg tgcggtgcca caatcggccc aactattatg agacgcaaat
     8881 tttaatgctt agtgactata gactatccgg gcaatgtttc caccgcgtat aacttttttt
     8941 acctaaaggt tacaattatt cagaattatc tttttaccga agcgcggtct tcagtataag
     9001 cattcatttt tcatatgatt tgtgcgcttg accgcaaact ggcatcacac ttgcgggaaa
     9061 ttcgataaat agcacatatg attaaaactc agatccaagt ggtcggatca cctgcatatc
     9121 ataagaagga aacaccatga ccctacgcaa gattctggca ctcacctgcc tgctgttgcc
     9181 gatgatggct tccgcacatc agttcgaaac cggtcagcga gtgccgccga ttggcattac
     9241 cgatcggggc gagttggtgc ttgataaaga tcagtttagc tacaaaacct ggaacagcgc
     9301 gcagttagtg ggaaaagtgc gagtactgca acatattgct ggtcgcacct ctgcaaaaga
     9361 gaaaaacgcg acgctgattg aagcgattaa atcagcaaag ttaccgcacg atcgttacca
     9421 gaccaccacc attgttaaca ccgacgacgc aattccgggt tccggcatgt ttgtgcgcag
     9481 cagtctggag agtaataaaa agctttatcc ctggtcgcag tttattgtcg atagcaatgg
     9541 cgtcgcgcgc ggtgcctggc agctggatga agaaagttcc gctgtagtgg tgctggataa
     9601 ggatggtcgc gtgcaatggg ccaaagacgg ggcgctcaca caggaagagg tgcagcaagt
     9661 gatggacctg ctgcaaaagt tgctcaaata atggcaataa cataaattct gcaaaaataa
     9721 ttttccccta tcgttatcta tgggataggg gaattagg
//
LOCUS       DAJTOR010000159         9693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTOR010000159 DAJTOR010000000
VERSION     DAJTOR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.159"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            45..728
                     /locus_tag="OO249_003366"
     CDS             45..728
                     /locus_tag="OO249_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5637541.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            772..5622
                     /gene="ehaG"
                     /locus_tag="OO249_003367"
     CDS             772..5622
                     /gene="ehaG"
                     /locus_tag="OO249_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HCR5637542.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALDGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSQVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            5990..7645
                     /gene="lldP"
                     /locus_tag="OO249_003368"
     CDS             5990..7645
                     /gene="lldP"
                     /locus_tag="OO249_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5637543.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7645..8421
                     /gene="lldR"
                     /locus_tag="OO249_003369"
     CDS             7645..8421
                     /gene="lldR"
                     /locus_tag="OO249_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5637544.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8418..9608
                     /gene="lldD"
                     /locus_tag="OO249_003370"
     CDS             8418..9608
                     /gene="lldD"
                     /locus_tag="OO249_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5637545.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADTHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         2469 a         2434 c         2506 g         2284 t
ORIGIN      
        1 tctaccggca ttgatcgctg gtcggaggaa ataaggattt ttacatgaac ttgaagaaga
       61 tttttttcag tgctgtcact gtttcagttc tctgcgcatt aacgggatgt gactacattg
      121 aagaaggcaa gccagaaagt agtttattga aacaacaaga agaacataac aataaaattg
      181 atctacttga aaaacaacaa gcacagctaa agagtcaact ggaaactatt caaaaacaac
      241 aaactggcat aataagcagt acaaagactt taactcacgt aattaaatcc gttaaagatc
      301 aacaaaacac ttttattttt accgagttta acccagcaaa aacgaagtat ttcatcctga
      361 acaatggttc ggtggcttta gcgggtcgag tgttatccat tgacgccaca gagaatggca
      421 gtgttattca tatttcactg gtcaacttat taagtacacc tatctcaaat attggtttta
      481 atgcgacatg gggtggtgaa aaacctgtcg atgccaaaga gtttgcccgg tggcaacaat
      541 tacttttcaa cacatcaatg aaatccacat tgaaattatt accaggtcaa tggcaagaca
      601 ttaatttgac cctgaagggt gtctcgccca ataacctggg atatctgaag ttagccatca
      661 acatggaaaa tattcagttc gacaatcttc cctctgctga aaatcggcag aaaagaagca
      721 aaaaataaat gcagttagta cacttataac aaggaatagc gagcatcatt aatgaacaaa
      781 atatttaaag ttatctggaa ccctgcgaca gggaattata ctgttaccag cgaaacggca
      841 aaaagccgtg gcaagaaatc tgggcgcagt aagctgttaa tttctgcgct ggttgcgggt
      901 ggaatgttgt cgtcgtttgg ggcattggcg aatgccggga atgacaacgg tcagggtgtt
      961 gattacggta gtggatcagc tggcgacggc tgggttgcta taggcaaagg ggcgaaagca
     1021 aatactttta tgaacaccag tggttccagt actgctgtgg gttatgacgc tatagctgaa
     1081 ggccaatata gctctgccat cgggtcaaaa acccatgcga ttggtggtgc atcaatggcc
     1141 tttggggtta gtgcaatatc agaaggcgat agaagtatag cactgggtgc ctcttcgtat
     1201 tcattgggcc aatactcaat ggccctcggc cgttattcaa aagcattggg taaattgtct
     1261 attgctatgg gggactcttc caaagcggaa ggagcaaacg ccattgccct gggaaatgcc
     1321 actaaagcta ctgagattat gagtattgct cttggcgaca ccgccaatgc gtcaaaagcg
     1381 tattcaatgg cgctgggagc aagtagcgtc gcatctgaag aaaacgcaat tgccctgggg
     1441 cgtagcagtg tagctagcgg tactgacagc ctcgcatttg gcagacaatc acttgccagc
     1501 gcagcgaacg ctattgcgat aggtgctgag accgaagccg ctgaaaatgc aactgctatt
     1561 ggcaataatg cgaaggcaaa agggactaat agcatggcaa tggggttcgg aagccttgcc
     1621 gataaagtca atactatcgc attaggaaat ggcagccagg ctctggcaga taatgcaatc
     1681 gccataggcc agggcaacaa agctgatggc gtggatgcca tcgctctggg taatggtagc
     1741 cagtcgagag gcttaaacac cattgcctta ggcacagcca gtaatgcaac tggtgataag
     1801 agtcttgcgc ttggtagtaa tagcagtgcc aacggtatta actctgtcgc gctgggcgca
     1861 gattccattg cggatttaga caataccgtc tctgtcggca atagttcatt aaaacgcaag
     1921 atcgttaatg tgaaaaatgg cgcgatcaag tctgacagtt acgatgccat taatggttca
     1981 caactttatg ccattagcga ctcggtagca aaaaggcttg gaggaggggc tgcagtagat
     2041 gttgatgacg gtactgttac agcaccaacc tacaatttaa aaaatggtag caaaaataac
     2101 gtaggggctg cgctcgctgt acttgatgaa aacaccctgc aatgggacca aaccaaaggc
     2161 aaatacagcg ctgctcatgg tactagtagc ccaactgcca gcgtaatcac cgatgttgcg
     2221 gatggcacga tttcagcctc cagtaaggat gcggttaacg gttcccaact gaaagctacc
     2281 aatgacgatg tcgaagccaa caccgccaat atcgctacta ataccagcaa cattgccacg
     2341 aatacggcaa atattgccac caataccacc aatatcacca acctgacgga ttccgttggt
     2401 gaccttcagg ctgatgccct gctctggaac gaaactaaaa aggcattcag tgcagctcac
     2461 ggccaggata ccaccagcaa aatcaccaac gttaaagatg ccgacctgac ggctgacagc
     2521 actgatgctg ttaacggctc tcagctgaaa accaccaacg atgctgtggc gacgaatacc
     2581 accaatatcg ccaataacac ttccaatatt gccactaaca ccaccaacat ctctaatctg
     2641 actgagacgg tgactaatct tggtgaggat gcgctgaaat gggataagga caatggtgta
     2701 ttcacggcag ctcatggcac cgagaccacc agcaaaatca ccaacgttaa agatggcgac
     2761 ctgacgactg gcagcaccga tgccgttaac ggctctcagc tgaaaaccac caacgatgcc
     2821 gtggcgacga ataccaccaa tatcgccact aacaccacca acatctctaa tctgactgag
     2881 acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtcttcact
     2941 gcagctcatg gcaacaatac cgccagcaaa atcaccaata tcctggacgg cacagtcact
     3001 gcaaccagtt ccgatgccat taacggtagc cagctttatg acttaagcag caatatcgcc
     3061 acctacttcg gcggcaatgc ttctgtgaat actgacggtg tgtttaccgg tccaacctac
     3121 aaaatcggtg aaacaaatta ttataacgtc ggcgatgcac tggctgcgat taactcctca
     3181 tttagcacgt ctctcggcga tgctctgctt tgggatgcca ccgcaggtaa attcagtgcc
     3241 aaacacggta ctaatggtga cgcaagcgtg atcactgatg tcgcagatgg tgaaatttca
     3301 gactccagtt ctgacgcagt aaacggctca caactccacg gcgtgagcag ttatgttgtt
     3361 gatgcgctgg ggggtggtgc cgaagtcaat gcagacggca ccatcactgc gccgacgtac
     3421 accattgcta atgctgatta cgataatgtc ggtgatgccc tgaatgctat cgataccact
     3481 cttgacgacg ctctgctctg ggatgcggac gccggtgaaa atggtgcatt tagcgccgct
     3541 cacggaaaag ataaaactgc cagtgtaatc actaacgtcg ctaacggtgc aatctctgct
     3601 gccagcagcg acgcgattaa cggctcacaa ctctatacca ccaataagta catcgctgat
     3661 gcgctggatg gtgacgcaga agtcaacgct gacggcacca tcaccgcacc gacttacacc
     3721 attgcgaacg ccgagtacaa caacgtcggt gacgctctgg atgcgcttga tgataacgcc
     3781 ctgctgtggg atgagactgc caatggcggt gctggagcct acaatgccag ccatgacggt
     3841 aaagccagca tcatcactaa tgtcgctaat ggcagtatta gtgaggacag taccgatgca
     3901 gtgaacggtt ctcagttgaa tgcgacgaat atgatgattg agcagaacac ccaaattatc
     3961 aatcagctcg ctggtaacac cgacgcaacc tatatccaag aaaacggtgc gggtattaac
     4021 tatgtgcgta ctaacgacga cggcttagcg ttcaacgacg ccagcgcaca gggtgttggc
     4081 gctacagcta taggttataa ctctgtcgcc aaaggcgata gcagcgtagc tattggtcag
     4141 ggcagctaca gcgacgttga tacgggtatc gccctaggta gcagctctgt ttccagccaa
     4201 gtgattgcca aaggctcccg tgacaccagc ataacggaaa atggcgttgt tattggttac
     4261 gacaccacgg atggcgaact gctcggtgca ttgtctatcg gtgatgacgg taaatatcgt
     4321 caaatcatca acgtagccga tggttccgaa gcccatgacg ccgttacggt tcgtcaattg
     4381 cagaatgcga ttggtgcggt cgcaaccacg ccgactaaat acttccacgc taattcaacg
     4441 gaagaagatt cactggcagt gggaactgac tcgctggcaa tgggtgcgaa aaccatcgtg
     4501 aatggcgata aaggtattgg tatcggttat ggtgcctacg tggacgcgaa tgcacttaac
     4561 ggcattgcca ttggtagcaa tgcgcaagtc attcatgtca acagtattgc gataggtaat
     4621 ggttctacga ccactcgtgg cgctcaaacc aattataccg cctacaacat ggacgcaccg
     4681 cagaactctg tcggtgaatt ctcagtcggt agtgcggatg gtcaacgtca gatcacaaac
     4741 gtcgcagctg gttcagcgga taccgatgcg gttaacgtgg gtcagttgaa agtcactgat
     4801 gagcgcgtag cgcaaaatac ccagagcatt actaacctga acaatcaggt cactaatctg
     4861 gatactcgcg ttactaatat cgaaaacggt attggcgaca ttgtcaccac cggtagcacc
     4921 aagtacttca agaccaacac cgatggcgta gatgccaacg cccagggtaa agatagcgtt
     4981 gctattggtt ctggttccat tgctgccgct gacaacagcg tcgcactggg taccggttcc
     5041 gttgcagagg aagaaaatac aatctctgta ggttcttcta ctaaccaacg ccgtatcacc
     5101 aacgtagctg ccggtgttaa tgccaccgat gcggttaacg tgtcgcagct gaaatcttct
     5161 gaagcaggcg gcgtgcgcta cgacaccaaa gctgatggtt ctatcgacta tagcaatatc
     5221 accctcggtg gtggcaacgg cagtacgact cgtatcagca acgtctccgc tggcgtcaac
     5281 aacaacgacg cggtgaacta cgcgcagttg aagcaaagcg tgcaggaaac gaagcaatac
     5341 accgatcagc ggatggttga gatggataac aaactgtcta aaactgaaag caagttgagc
     5401 ggtggtatcg cttctgcaat ggcaatgacc ggtctgccgc aggcttatac accgggtgcc
     5461 agcatggctt ctattggtgg cggtacttac aacggtgaat cggcagttgc tttaggtgta
     5521 tcgatggtga gcgccaatgg tcgttgggtc tacaaattac aaggtagtac caatagccag
     5581 ggtgaatact ccgccgcact cggtgccggt attcagtggt aatgatccat taagttagtg
     5641 tgactaaggg caaacgccaa attgcctgat gcgttacgcg catcaggcaa gataaggcgc
     5701 tccgggtcaa caatctaagg gcaattctct gatgaggatt gcccttttct ttaccagaca
     5761 tctcccccca caagaattgg ccctaccaat tcttcgctta tctgacctct ggttcacaat
     5821 tccccaatta aaactcacat caatattgcc aatacacaac atttagttaa ccattcattg
     5881 tcattatccc tacacaacac aattggcagt gccactttta cacaacgtgt gacaaggaga
     5941 tgagcaacag actcattaca cgatgtgcgt ggactccagg agacctgcaa tgaatctctg
     6001 gcaacaaaac tacgatcccg ccgggaatat ctggctttcc agtctgatag catcgcttcc
     6061 catcctgttt ttcttctttg cgctgattaa gctcaaactg aaaggatacg tcgccgcctc
     6121 atggacggta gcaatcgccc ttgccgtggc tttgctgttc tataaaatgc cggtcgctaa
     6181 cgcgctggcc tcggtggttt atggcttctt ctatggtttg tggcccatcg cgtggatcat
     6241 tattgcagca gtgttcgtct ataagatctc ggtgaaaacc gggcagtttg acatcattcg
     6301 ctcgtctatt ctttcgataa cccctgacca gcgtctgcaa atgctgatcg tcggtttctg
     6361 tttcggcgct ttccttgaag gagccgcagg ctttggcgca ccggtagcaa ttaccgccgc
     6421 attgctggtc ggcctgggtt ttaaaccgct gtacgccgcc ggactgtgcc tgattgttaa
     6481 caccgcgcca gtggcatttg gcgcgatggg cattccaatc ctggttgccg gacaagtaac
     6541 aggcatcgac agttttgaga ttggccagat ggtggggcgt cagctaccgt ttatgaccat
     6601 tatcgtgctg ttctggatca tggcgattat ggacggctgg cgcggtatca aagagacgtg
     6661 gcctgcggtc gtggttgcgg gcggctcgtt tgccatcgct cagtacctta gctccaactt
     6721 cattgggccg gagctgccgg acattatctc ttcgctggta tcactgctct gcctgacgct
     6781 gttcctcaaa cgctggcaac cagttcgcgt attccgcttc ggtgacttgg gggcgtcaca
     6841 ggttgatatg acgctggccc acaccggtta caccgcgggt caggtgttac gtgcctggac
     6901 accgttcctg ttcctgaccg ctaccgtaac actgtggagc atcccgccgt ttaaagccct
     6961 gttcgcatcg ggtggtgcgc tgtatgagtg ggtgatcaac attccggtgc cgtacctcga
     7021 taaactggtt gcccgtatgc caccagtggt cagcgaggct acagcctatg ccgccgtgtt
     7081 taagtttgac tggttctctg ccaccggcac cgccattctg tttgctgcac tgctctcgat
     7141 tgtctggctg aagatgaaac cgtctgacgc tatcagcacc ttcggcagca cgctgaaaga
     7201 actggctcta cccatctact ccatcggcat ggtgctggcg ttcgccttta tctcgaacta
     7261 ttccggactg tcatcaacac tggcactggc actggcgcac accggtcatg cattcacctt
     7321 cttctcgccg ttcctcggct ggctgggggt attcctgacc gggtcggata cctcatctaa
     7381 cgccctgttc gccgcgctgc aagccaccgc agcacaacaa attggcgtct ctgatctgtt
     7441 gctggttgcc gccaatacca ccggtggcgt caccggtaag atgatctccc cgcaatctat
     7501 cgctatcgcc tgtgcggcgg taggcctggt gggcaaagag tctgatttgt tccgctttac
     7561 tgtcaaacac agcctgatct tcacctgtat ggtgggcgtg atcaccacgc ttcaggctta
     7621 tgtcttaacg tggatgattc cttaatgatt gttttaccca gacgcctgtc agacgaggtt
     7681 gccgatcgtg tgcgggcgct gattgatgaa aaaaacctgg aagcgggcat gaagttgccc
     7741 gctgagcgcc aactggcgat gcaactcggc gtatcacgta attcactacg cgaggcgctg
     7801 gcaaaactag tgagcgaagg cgtgctgctc agtcgacgcg gcggcgggac gtttattcgc
     7861 tggcgtcatg acacatggtc ggagcaaaac atcgtccagc cgctaaaaac gctgatggcc
     7921 gatgatccgg attacagttt cgatattctg gaagcccgct acgccattga agccagcacc
     7981 gcatggcatg cggcaatgcg cgccacacct ggcgacaaag aaaagattca gctttgcttt
     8041 gaagcaacgc taagtgaaga cccggatatc gcctcacaag cggacgttcg ttttcatctg
     8101 gcgattgccg aagcctcaca taacatcgtg ctgctgcaaa ccatgcgcgg tttcttcgat
     8161 gtcctgcaat cctcagtgaa gcatagccgt cagcggatgt atctggtgcc accggttttt
     8221 tcacaactga ccgaacaaca tcaggctgtc attgacgcca tttttgctgg tgatgccgac
     8281 ggggcgcgta aagcaatgat ggcgcacctt agttttgttc acaccaccat gaaacgattc
     8341 gatgaagatc aggctcgcca cgcacggatt acccgcctgc ccggtgagca taatgagcat
     8401 tcgagggaga aaaacgcatg attatttccg cagccagcga ttatcgcgcc gcagcgcaac
     8461 gcattctgcc gccgttcctg ttccactata tggatggtgg tgcatattct gaatacacgc
     8521 tgcgccgcaa cgtggaagat ttgtcagaag tggcactgcg ccagcgtatt ctgaaaaaca
     8581 tgtccgactt aagcctggaa acgacgctgt ttaatgagaa attgtcgatg ccggtagcac
     8641 tggcaccggt gggtttgtgt ggcatgtatg cgcgtcgtgg cgaagttcag gcagccaaag
     8701 cggcggacac gcatggcatt ccgtttactc tctcgacggt ttccgtttgc ccgattgaag
     8761 aagtcgcgcc agccatcaag cgcccaatgt ggttccagct ttatgtactg cgcgatcgcg
     8821 gctttatgcg taacgcgctg gagcgagcaa aagcagcggg ttgttcgacg ctggttttca
     8881 ccgtggatat gccgacaccg ggcgcacgct accgtgatgc gcattcaggt atgagcggcc
     8941 caaatgcggc aatgcgccgc tacttgcagg cggtgacgca tccgcaatgg gcgtgggatg
     9001 tgggcctgaa cggtcgtcca catgatttag gtaatatctc agcttatctc ggcaaaccaa
     9061 ccggactgga agattacatc ggctggctgg ggaataactt cgatccgtcc atctcatgga
     9121 aagaccttga gtggatccgc gatttctggg atggcccgat ggtgatcaaa gggatcctcg
     9181 atccggaaga tgcgcgcgat gcagtacgtt ttggtgctga tggaattgtg gtttctaacc
     9241 acggtggccg ccagctggac ggtgtactct cttccgctcg tgcgttgccc gctattgcag
     9301 atgcggtgaa aggtgatatc gccattctgg cggatagcgg aattcgtaac gggcttgatg
     9361 tcgtgcgtat gattgcgctc ggtgccgaca ccgtactgct gggtcgtgct ttcctgtatg
     9421 cgctggcaac agcgggccag gcgggtgtag ctaacctgct aaatctgatc gaaaaagaga
     9481 tgaaagtggc gatgacgctg actggcgcga aatcgatcag tgaaattacg caagattcgc
     9541 tggtgcaagg gctgggtaaa gagttgcctg cggcactggc tcccatggcg aaagggaatg
     9601 cggcatagtc gtttgcgccc ccgttacttt attcgtaccc cttataatgg ggtgttagcc
     9661 agccagaccc ggcatgatta ctgcccccag tcg
//
LOCUS       DAJTOR010000160         9581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTOR010000160 DAJTOR010000000
VERSION     DAJTOR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.160"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(272..1636)
                     /gene="tdcG"
                     /locus_tag="OO249_003371"
     CDS             complement(272..1636)
                     /gene="tdcG"
                     /locus_tag="OO249_003371"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5637546.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGALSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1708..2097)
                     /locus_tag="OO249_003372"
     CDS             complement(1708..2097)
                     /locus_tag="OO249_003372"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5637547.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(2111..4405)
                     /gene="tdcE"
                     /locus_tag="OO249_003373"
     CDS             complement(2111..4405)
                     /gene="tdcE"
                     /locus_tag="OO249_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5637548.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFEAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4439..5647)
                     /gene="tdcD"
                     /locus_tag="OO249_003374"
     CDS             complement(4439..5647)
                     /gene="tdcD"
                     /locus_tag="OO249_003374"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5637549.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5673..7004)
                     /gene="tdcC"
                     /locus_tag="OO249_003375"
     CDS             complement(5673..7004)
                     /gene="tdcC"
                     /locus_tag="OO249_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5637550.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(7026..8015)
                     /gene="tdcB"
                     /locus_tag="OO249_003376"
     CDS             complement(7026..8015)
                     /gene="tdcB"
                     /locus_tag="OO249_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5637551.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(8114..9052)
                     /gene="tdcA"
                     /locus_tag="OO249_003377"
     CDS             complement(8114..9052)
                     /gene="tdcA"
                     /locus_tag="OO249_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5637552.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            9241..>9581
                     /gene="tdcR"
                     /locus_tag="OO249_003378"
     CDS             9241..>9581
                     /gene="tdcR"
                     /locus_tag="OO249_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5637553.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         2558 a         2437 c         2185 g         2401 t
ORIGIN      
        1 tttctcgctc aatattttgt agggcttatt atttttttcc gagccgcatc aaggcgatat
       61 gcggtctacg tttttgcagg cgagttatta gaataaagaa atgatacgcg cgaggtcaga
      121 gaaaatgttc acaatcaaga ctttgaaata acggagatgg ataagaattt tctactaaat
      181 taatcgcaag agaatattca ttctctattt gcggcagatc acaaaaaaag gtgcacattt
      241 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
      301 ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
      361 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg
      421 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
      481 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
      541 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg
      601 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
      661 acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg
      721 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
      781 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
      841 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct
      901 gacgacgcag cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag
      961 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt
     1021 caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca
     1081 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
     1141 acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
     1201 cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc
     1261 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
     1321 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
     1381 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa
     1441 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgccccatac agatcgacca
     1501 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
     1561 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
     1621 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
     1681 ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
     1741 cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc
     1801 ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc
     1861 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
     1921 cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc
     1981 ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
     2041 ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
     2101 tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac
     2161 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
     2221 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattatgttg acgttgaggt
     2281 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
     2341 tagttttacg tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg
     2401 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
     2461 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
     2521 gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca
     2581 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
     2641 agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
     2701 gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca
     2761 cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc
     2821 atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
     2881 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
     2941 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg
     3001 gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
     3061 agagcagcgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
     3121 ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca
     3181 gatcgtcatt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
     3241 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc
     3301 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
     3361 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
     3421 caaattccgg tgtacgcagg aagcgtacca tacggatctt catgatgaag tgatcgatca
     3481 gttcctgtgc ctgctgctca ttgagtacgc cagcttcaaa gtcgcgctca atgtagatat
     3541 cgaggaacga tgccgtgcgg cccagcgaca tcgcgccgcc attttgcgat ttcactgccg
     3601 ccagataagc gaagtagagc cactgcaccg cttcttgcgc attctgcgcc gggcgagaga
     3661 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
     3721 ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact
     3781 ggagatcggc aaattgcagt tcgcgttcac gtaccagata actgatgcca tacagcgcta
     3841 cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca
     3901 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
     3961 gggttttacg cagatcggta aacagatatt caaattcact gtccatttca cggccatagg
     4021 cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtagcgca cgtttcaacg
     4081 gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat
     4141 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt
     4201 cgatacggat accttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg
     4261 cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
     4321 taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat
     4381 cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt
     4441 atgcaaattc tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt
     4501 tttcttcgtt agtcggaata acagcgcaaa tgacacgcgc attttcactg gaaacaattc
     4561 gctcaccaca ggagttagag cgattattca tttctgtatc aatctctacg cctaatacag
     4621 ccaaatgttc catgaccaga cgacgaatta agcttgaatt ctctcctatt ccgccggtga
     4681 atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc
     4741 ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt
     4801 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
     4861 ctacgcgttc caagtcaccc aagctctggt tggtttggct ggcgacccag gacatcgcac
     4921 caaaatcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca
     4981 ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca ttgccaagat
     5041 gcgccacaac caggccggag tcatcttccg ctagattcag caaagaatgt gcgcgctggg
     5101 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
     5161 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
     5221 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
     5281 aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgacct
     5341 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
     5401 ttaaggccac actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
     5461 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
     5521 cattttctga gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat
     5581 cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
     5641 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg
     5701 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
     5761 agagacggtg ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc
     5821 ggtgcgccca tggcttcaat aaggtcgagg atgttcgggt tggcgtaggc aacaacccag
     5881 gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt
     5941 ttgtcgcctt tataaccgaa cttcagaatc agaccattca agccttccag cgtccccaga
     6001 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
     6061 agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa
     6121 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaac
     6181 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
     6241 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
     6301 ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa
     6361 atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
     6421 tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc
     6481 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
     6541 aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc
     6601 ggtgcaaatc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
     6661 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
     6721 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
     6781 agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac
     6841 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
     6901 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
     6961 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
     7021 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
     7081 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
     7141 aatgcagcac atgccagagc acctgcgcct tcggtgacga ctttattgcg ctgaattaag
     7201 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
     7261 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
     7321 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
     7381 ttttctgact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
     7441 ataccagcaa ttaaaccacc accaccaatt ggcacaatca cgttatcgac atcatagaga
     7501 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
     7561 ggtgggataa aaatacgacc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
     7621 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
     7681 gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
     7741 agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
     7801 gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
     7861 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag
     7921 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatatca
     7981 tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
     8041 atatgtagat aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa
     8101 taaaaacaaa taactaacca acttctatta atagccttcg tctacaccca ttataagatg
     8161 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
     8221 accataccgc ggcatattgt gccacaggta atgtttcttc aactggaata gtaataaatt
     8281 gatttgaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga
     8341 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
     8401 catttctttg taacgtagta agaagctcgc tgtagtaccc catattggtt tgtggcaaca
     8461 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
     8521 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
     8581 tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
     8641 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
     8701 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
     8761 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
     8821 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
     8881 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
     8941 cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga
     9001 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
     9061 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc
     9121 gaccactcac aaattaactt tcattacttt ccagggtatc gttattacaa ttaaatatct
     9181 ttaaatatca acaagttaaa acataaaaat cagcataaac cctgattttt atcaaaaaaa
     9241 atgacgggga taaccatatt ttatggcgat aacatcattc gttatgtggt taatacaaaa
     9301 aaggggctga gaccatattt caagcaatta ccggataatt accaggcaaa atttgagtta
     9361 aatttaatga gcaagttctc aaattttatt ataaataaac cattttcagc gataaatact
     9421 gcggcacgtc acattttttc acgctatttg ttggagaaca aacatttatt ttatcaatat
     9481 tttaaaattt cgaatacagg tattgatcat ctcgaacaat tgattaacgt caactttttc
     9541 tcttctgaca ggacgtcatt ttgtgaatgc aatcgttttc c
//
LOCUS       DAJTOR010000161         9544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTOR010000161 DAJTOR010000000
VERSION     DAJTOR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.161"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..501
                     /gene="fklB"
                     /locus_tag="OO249_003379"
     CDS             <1..501
                     /gene="fklB"
                     /locus_tag="OO249_003379"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5637554.1"
                     /translation="GIADALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEG
                     VKYLEENAKKEGVNSTESGLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVA
                     RGEPAEFPVNGVIPGWIEALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEV
                     ELLEIL"
     gene            810..2222
                     /gene="cycA"
                     /locus_tag="OO249_003380"
                     /gene_synonym="dagA"
     CDS             810..2222
                     /gene="cycA"
                     /locus_tag="OO249_003380"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5637555.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFLG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(2267..2929)
                     /gene="ytfE"
                     /locus_tag="OO249_003381"
     CDS             complement(2267..2929)
                     /gene="ytfE"
                     /locus_tag="OO249_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5637556.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYNMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(3037..4002)
                     /locus_tag="OO249_003382"
     CDS             complement(3037..4002)
                     /locus_tag="OO249_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5637557.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIVVRAKPEKPLTESVSES"
     gene            complement(4110..4970)
                     /gene="qorB"
                     /locus_tag="OO249_003383"
     CDS             complement(4110..4970)
                     /gene="qorB"
                     /locus_tag="OO249_003383"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5637558.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLTDSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            5059..5439
                     /locus_tag="OO249_003384"
     CDS             5059..5439
                     /locus_tag="OO249_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5637559.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(5568..7511)
                     /gene="cpdB"
                     /locus_tag="OO249_003385"
     CDS             complement(5568..7511)
                     /gene="cpdB"
                     /locus_tag="OO249_003385"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5637560.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDRHIAFASPDE
                     NRSVLTAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            7701..8441
                     /gene="cysQ"
                     /locus_tag="OO249_003386"
     CDS             7701..8441
                     /gene="cysQ"
                     /locus_tag="OO249_003386"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5637561.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            8653..>9544
                     /locus_tag="OO249_003387"
     CDS             8653..>9544
                     /locus_tag="OO249_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637562.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKE"
BASE COUNT         2326 a         2303 c         2444 g         2471 t
ORIGIN      
        1 ggtattgccg atgcgctgga aggcaaacat ccggctgttc cggttgatgt ggtgcatcgc
       61 gcgctgcgtg aaatccacga acgcgccgat gccgttcgtc gtcagcgttt ccaggcgatg
      121 gctgctgaag gtgtgaaata cctggaagaa aacgccaaaa aagaaggtgt gaatagcacc
      181 gaatctggcc tgcaattccg cgtgatcaac cagggtgaag gcgcaattcc ggcacgtacc
      241 gaccgcgttc gtgttcatta caccggtaaa ctgatcgacg gcaccgtgtt tgacagctcc
      301 gttgctcgtg gtgaacccgc tgaattcccg gttaacggcg tgatccctgg ctggattgaa
      361 gcactgactc tgatgccggt aggttctaaa tgggaactga ctatcccgca ggaactggca
      421 tatggcgagc gcggcgcagg tgcatccatc cctccgttca gcaccctggt gtttgaagtc
      481 gaactgctgg aaatcctcta agcagcgcat tctgttcccc tcgaaccaga ggggagcagg
      541 cattcagcaa taaacccttc agtttgccaa acggcgctat tttgtgttgc aaagaccccg
      601 taagcgtgta tttttgtgag atgtttcgcg ttatcaccgt gatatgacac tcactttaaa
      661 cataaaatta acattagatc taaatcttag tattcatccc gcgtattgtt acctaatatc
      721 gatgagtccc gatacagatt cgtcgtatca tagactgact aaaggccgta gagcctgaac
      781 aacacagaca ggtacaggaa gaaaaaaaca tggtagatca ggtaaaagtc gttgccgatg
      841 atcaggctcc ggctgaacag tcgctacggc gcaatctcac aaaccgacat attcagctta
      901 ttgccattgg cggtgccatt ggtacggggt tgtttctggg gtctggcaaa acgattagcc
      961 ttgccgggcc gtcgatcatt ttcgtttata tgatcattgg ttttatgctc tttttcgtga
     1021 tgcgggcaat gggggaattg ctgctttcga atctggaata caaatctttt agtgacttcg
     1081 cttccgattt actcgggccg tgggcaggat atttcactgg ctggacttac tggttctgct
     1141 gggttgtaac cggtatggca gacgtggtgg cgatcacggc ttatgctcag ttctggttcc
     1201 ccgatctctc cgactgggtc gcctcgctgg cggtgatagt gctgctgctg acgctcaacc
     1261 ttgccaccgt gaaaatgttc ggtgagatgg agttctggtt tgcgatgatc aaaatcgtcg
     1321 ccatcgtgtc gctgattgtc gtcggcctgg tcatggtggc gatgcacttt cagtcaccga
     1381 ctggtgtgga agcgtcattc gcgcatttgt ggaatgacgg cggctggttc ccgaaaggtt
     1441 taagtggctt ctttgccgga ttccagatag cggttttcgc tttcgtgggg attgagctgg
     1501 taggtacaac agctgcggaa accaaagatc cggagaaatc actgccacgc gcgattaact
     1561 ccattccgat ccgtatcatt atgttctacg tcttcgcgct gattgtgatt atgtccgtga
     1621 caccgtggag ttcggtagtc ccggagaaaa gcccgttcgt tgagctgttt gtgttggtag
     1681 gtttgcctgc ggctgccagc gtgatcaact ttgtggtgct gacctctgcg gcgtcttccg
     1741 ctaacagcgg cgtcttctct accagccgta tgctgtttgg tctggcgcag gaaggtgtgg
     1801 caccgaaagc gttcgctaaa ctttctaagc gcgcagtacc agcgaaaggg ctgaccttct
     1861 cctgcatctg cctgctcggt ggcgtggtga tgctgtatgt gaatcctagc gtgattggtg
     1921 cgttcacgat gattacaacc gtttccgcga ttctgtttat gttcgtctgg acgattatcc
     1981 tttgctcgta cctggtgtac cgcaaacagc gtcctcatct gcatgagaag tcgatctaca
     2041 agatgccgct cggcaagctg atgtgctggg tatgtatggc gttctttgtg ttcgtggtcg
     2101 tgttgctgac actggaagat gacactcgcc aggcgctgct ggttaccccg ctgtggttta
     2161 tcgcactggg gctgggctgg ttgtttattg gtaagaagcg ggctgctgaa ctgcggaaat
     2221 aaccgcatta tcatgctgga tggcgcaatg ccatccagct tttagatcac tcacccgcca
     2281 gcgcgcgtgg gaacagtaca ttgttttcca gactgatgtg atccatcagg tcatcaatca
     2341 gttcattaat gccgttatac atcgctttcc aggtggtgca ggcttctggc ggcggtgtga
     2401 cgttattggt agtgtgtttg atcacttcca gcagttcgcc cgcttcatcg tgctcgcttt
     2461 ccattacgct gattggcccc attgcctggc tgcccatgcc ttgtttgatc atcgggaaga
     2521 ggatctgctc ttctttcatc atgtggctgg aaagctcttc atgcagcatg gtcaggtatt
     2581 ttgtcagccc ttttggcacg ctcggtttgt cggcgtgaac gcgctcgact ttagtcgctt
     2641 gcaggatcag ctccggcagt tgctcgcggt gacgatcgtg gtagcgcacg atgatatggt
     2701 caatgatttc tgccagcggg gcgctacgcc agtctttctc aatcggttgt tcagcaagct
     2761 ttgccagttc agcttcaatg acctcaacat ccagttcttt acgtgccgcc gcgcgcgcca
     2821 gcgtctgctt accgccacag cagtaatcca tattatattt acgaaacaga gctgaagcgc
     2881 gaggaataga gagcgccagt tcacctaaag gttggtcgcg ataagccata gctgatacct
     2941 cattcttaat aagataagat gtattttaaa tacatcttta aggcaaaagc tataaccctt
     3001 acgtagcaga ggcttattta actcacagcc agtatgtcaa ctttctgaga cagattcagt
     3061 taaaggcttt tccggttttg ctctgactac aatgaccacg ccaaccacta acagcgcgat
     3121 accgctcagc gtcatcagcg gcggcatttg ctggcgtatc aaaaaggtgt acagcaaacc
     3181 tgccagtgtt tcgaaaacga tcagcggccc gagaatcact gtcggtaatc gctggctggc
     3241 gacgttccag cagagtgcgc caacccatga gcaaagcacg gctatcgcaa ccatcagact
     3301 aataaacacc agcggacggg ggccaaaagg tagggagaag tccggcgttt gcgtattcag
     3361 ccagtaacag gcgacgagat agccgatgag agaaaccggc agtgtgacca gcgcctgcgc
     3421 cgtcgcccac atcatcggat gtttgtcggg attctcccgc agccagcggg cgttgcgcag
     3481 ggcataccat gcccagcaga ccacggaaac taacgctagc acgatgcctg aggtataacg
     3541 tgcccagtca aaatcgggga gtccgtggtt taactcagca atattcacac acgccaggcc
     3601 gatgccaata caaaccagtg ccggggcgag ttttccccac gcgagtttgc cgtcgcgctg
     3661 gctataaagc agattggcaa aaacgggaat caccaccggc agggtgccga taatcatcgt
     3721 ggaaacaggc gcaccagtac gttgaatggc actggcaagg cagaagtaat agatgaggtt
     3781 gcccatcata gtgagcatca aggcggtaag ccagtcgcga cgcgccaact gacgcagacg
     3841 cacgcgtccc agccaggcaa tgggcagcgc aattaaccct aacgccagat aacgccccat
     3901 cgactgcaac atcgccgggt attccggtac gatcaacggc ccgacaaaaa taagccccca
     3961 catcaaccct gctaacaggg cgtacagcac gccgctaatc attactggca tccattggtc
     4021 tgtcagaaga aagttcagga agcagacatg attgctcccg gcatcccgtc attattagag
     4081 aggatgcggg aggatgccac tttaattaac tagttattaa cattaaaaag atggcttacg
     4141 ctttcggcta acgttgtcgt tgggcggcca atcaatttgc taagcgtttt gctgttatca
     4201 aacagaccac ctttcgatgc gccaacgtca gaatccgtca gcatatccgc cagtccgtcg
     4261 ggcagtccga cgcttttcag tgccgcggcg aaatcggctt cgctcagatt ttgataggta
     4321 acctgtttgc cgctctgttt ggtcagctct gccgctaact gtgtcaacgt ccaggcacta
     4381 tcgcccgcca gttcgtaaac cttgccttcg tgaccggctt cgctaatcac gcgtgccgca
     4441 gctgccgcat aatctgcccg cgttgctgag gcgattttgc catcgcccgc cgcaccgata
     4501 aatacgccgt gttccagtgc tgccggggcg ctggcgaggt agttttcgct gtaccagccg
     4561 ttgcgcagca gggtgtaaac gatgccagaa tcagccaaca ttttctccgt ctcaatgtgc
     4621 tcatcggcga ggccgagcgg ggaggtatct gcatgtagca ggctggtata agcgataaat
     4681 ttcacgccag ccgcctttgc ggcattaata acattacgat gctgcggggc acgttgaccc
     4741 acttcgctgg aagagatcag cagtagtttt tccactccct gaagtgcaga tgtcagtgcg
     4801 gcttcatcgc cgtagtcagc ctgacgcacg gtaatgcctt gtgctgccag agcctgggct
     4861 tttgccggat tacgaacgat agccactatt tggctggcag gaaccgtttt catcaaggat
     4921 tcaataatat agtgaccaag ttggccagtg gcaccagtaa tagcgatcat gggaagtctc
     4981 catcgttttg tcttgtgttt aaatacgcta acacctaaac ttacttttag taagtacgta
     5041 caaaaaggta agtatgaaat gagtcaggtt agcctgtcgc aacaactgaa agagggtaat
     5101 ctctttgcgg aacagtgccc gtcgcgcgag gtgttgaaac acgtcaccag ccgttggggg
     5161 gtgttgattc tggtggcgct acgcgaaggt actcatcgct ttagcgacct gcggcgcaaa
     5221 atcggcgggg tgagcgaaaa gatgcttgcg cagtcgttac aggcgttaga acaggatggt
     5281 tttcttaacc gtatcgcgta tccggtggtg ccaccgcatg tggaatatag cctcacgccg
     5341 ttgggcgagc aggtgagcga aaaggttgct gcactggcgg actggattga gttgaatttg
     5401 cccgaggtgt tggcggtgcg ggatgaacgt gcggcataac tgccggatgc gctgagctta
     5461 tctggtctac ggtgcatggc tggtagggcg gataagatgc gccagcatcg catccgccaa
     5521 taaatgccgg atgcggttga taatttatag accgaaaaag aagtgtttta cttactcaaa
     5581 tccacctgat aaatcgcaaa cccgatatca tcggtcgcga ctttattcat cggatattgc
     5641 cctttctctt taataaacgc tgcggcttta tctgacgggg aagtttcgaa acggatatcc
     5701 agtttcttat cgccagctat cggtgctaaa cgccagttgt tatctgccgc cgggtgaatt
     5761 tctcccgcac gtttcgactc atcagcaatc cacgctgtca gcaccgagcg gttctcatcc
     5821 ggtgaagcaa aagcgatatg tctgtcgccc gtaccggcaa atttgccgcc gtaagcgcga
     5881 tagttattgg tggcaacgag gaacatagcg ttcggatcaa tcggcttgcc attaaaggtc
     5941 aggttcttaa tccgctctgc attggcatta atcatctggc actcgccgtc atagcgggcg
     6001 ggttgggtaa catcaatctg ataattcaca ccatcaataa catcaaagtt ataagtgcgg
     6061 aaaccatccc agttgatgag tgactgtggt ttcgtgctgt taggatcaat ctggttaaac
     6121 tgtccggcgg agcactccag ccactctttc acctctttac cgctggcttt cactacaatc
     6181 agcgtattgg ggtagagata aagatcggcg gcattacgga aggtcaactg gcctttttcc
     6241 acctccacat agcttgccgg gtcatttttg cgaccaccaa ctttaaacgg tgcggcagct
     6301 gaaagcaccg gcagttttgc cagatccgga tcgccctgaa tgtaatgttc gacatacgct
     6361 ttttgcgcgt tgttcacgac ttgcacggtc ggatcgtcct gcaccagcgc cagatagcta
     6421 tacatattgt cggcagattt accgattggc ttgctgacga actggcgtgt ggcatcgtga
     6481 tcggctttga gtgtttctac cagcttgctg tcttccgccg cgagggattt tttattggcg
     6541 atgtcgtaaa tcggccgtgc ttccgctttc gcctgcgtca cctgccattt accgctgtca
     6601 ttactgagtt gtaagtcgac caccccaaga tgatcgcccc acatgcccgg cattaccgcc
     6661 ggaacaccat tcagcgtgcc tttggtgata tcagcctctt cgatatcagc aaaatcttta
     6721 cccgggaaaa cggcgtgagc atggccaaac ataatggcgt taacgcccgg aatttcactg
     6781 aggtaataaa ctgagttttc cgccattact ttatacggat cggcagacag cccggaatgc
     6841 gccagaacga caacgacatc ggcacctttc tcgcgcattt caggcacgta tttgcgcacg
     6901 gtttcggtaa tatcattcac cgtcactttt ccggataaat tagctttatc ccagcccatg
     6961 atttgcggcg gcacgacgcc aatatagcca atcttcagcg tctgtttttt tccgtcttta
     7021 tcgaccactt cggtatcttt aattaaatac ggtgtaaaca ttggctgttt ggttctggcg
     7081 tcaatgacgt tggcatttac ataagggaat ttcgctcccg ccagcgcatt tttcaggtaa
     7141 tccagaccgt agttaaattc atgattgccg agtgtaccga ccgtataatc cagcgtattc
     7201 agcgccttat aaaccggatg aacatcacct gcttttaatc ctttcgccga catgtaatcg
     7261 gccagcggac tcccctgaat caaatcgccg ttatcgacca gtacgctgtt tttcacttca
     7321 ttgcgggcat cgttaatcag gcttgccgta cgtaccagtc cgaatttttc cgtggcggtg
     7381 tctttgtaat aatcgaaatc catcatgttg ctatgcagat cagtggtttc catgatacgc
     7441 agatcgaccg tcgctgcatt cacactggcg gcaatcagcg tggccaggag cgttgcgcta
     7501 aacttaatca tcagggacat ccttttatca tcgggaatac gaaagaaaag ggagaataaa
     7561 cgtcttactt atagaacagt gaagaatgcc acaattttac gctttgaaaa tgatgacact
     7621 atcacagttg gcgcattcat taacgatagg gtataagtaa aacaataagt taacaccgct
     7681 cacagagacg aggtggagaa atgttagatc aagtatgcca gcttgcacgg aatgcaggcg
     7741 atgccattat gcaggtctac gacgggacga aaccgatgga cgtcgtcagc aaagcggaca
     7801 attctccggt aacggcagcg gatattgccg cacacaccgt tatcatggac ggtttacgta
     7861 cgctgacacc ggatattccg gtcctttctg aagaagatcc tcccggttgg gaagtccgtc
     7921 agcactggca gcgttactgg ctggtagacc cgctggatgg tactaaagag tttattaaac
     7981 gcaatggcga attcaccgtt aacattgcgc tcattgacca tggcaaaccg attttaggtg
     8041 tggtgtatgc gccggtaatg aacgtaatgt acagcgcggc agaaggcaag gcctggaaag
     8101 aagagtgcgg tgtgcgcaag cagattcagg tccgcgatgc gcgcccgccg ctggtggtta
     8161 tcagccgttc ccatgcagat gcggagctga aagagtatct gcaacagctt ggcgaacatc
     8221 agaccacgtc catcggctct tcgctgaaat tctgcctggt ggcggaagga caggcgcagc
     8281 tgtacccgcg cttcggacca acgaatattt gggacaccgc cgctggacat gctgtggccg
     8341 cagctgccgg agcgcacgtt cacgactggc agggtaaacc gctggattac actccgcgcg
     8401 agtcgttcct gaatcctggg ttcagagtgt ctatttacta aattcagatg gcagaaacag
     8461 tgtatttcct gattctgcca tcctgatttc tcccaaccta aaaagttata aataaaaaga
     8521 gattgtattt aaagtgcaaa aattcaattg ctaataagtt acattttaat aatgagcgtt
     8581 ttttgatagt ttacttctat aatgagatat ttaatggcga cataaaataa ccaaataaaa
     8641 taaggtagcc atatgttacc caggatcaga cacaataatt ttattggtgc ggtggagtta
     8701 ttcgtaaaat catcgcatac aaaaacacat tcaaacaatt tttttaataa tattcagcat
     8761 gcatttaaga aaaaagattg ggtttcgaat tatgatagcc tgttaacttt gtgggagttt
     8821 tttcgttgcg caactcagat tgataaagat agttatcaag tgctttcatc aaaaaatgaa
     8881 actgttaaag ccatggataa gtttcttatc agtttcagcc ttaaagataa cggtgccgaa
     8941 tatacaatga cattgcgagg cagtggattt gaatacgaag aaatccccat aacaataaat
     9001 gagtataact cattcatgga tttcaaaaac cgtgaatttc cgttagaaca aaacagaaga
     9061 ttgtatgctt gggatattct gcagaaaaaa cagtctgaca taccgaaaag gattaaaggt
     9121 tatattcacc aggcgattgg tgacgtgtct ttaggttatg ccttattgga ggatatagta
     9181 agcaaattaa aacggggtaa atttgaactc caaataccag gaggtggaat taaagaatgc
     9241 gatggatggt atatttacga aaaaataatt gatgataatt ttgccatagt catagaatct
     9301 ttgggctttg cgcttaaaat ctatggaggg gatgaacgtt ttcgtaatgg ttcctctgtt
     9361 gttttggagg atgaagatta ctctcttatt tataattttc tggttaatgc aggttgccaa
     9421 caagtcgaat tagccgaaca agtggatgcg atagtaagtg cgaatctggc cgtagatagt
     9481 gatattaccg aagaggagat ttgcgagaaa tataaatcaa ccattgaggc gtttaaaaaa
     9541 gagc
//
LOCUS       DAJTOR010000162         9535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTOR010000162 DAJTOR010000000
VERSION     DAJTOR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.162"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..853)
                     /locus_tag="OO249_003388"
     CDS             complement(<1..853)
                     /locus_tag="OO249_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HCR5637563.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            1196..2026
                     /locus_tag="OO249_003389"
     CDS             1196..2026
                     /locus_tag="OO249_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5637564.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            2058..2993
                     /locus_tag="OO249_003390"
     CDS             2058..2993
                     /locus_tag="OO249_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5637565.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            3086..3328
                     /locus_tag="OO249_003391"
     CDS             3086..3328
                     /locus_tag="OO249_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5637566.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            3318..4769
                     /gene="panF"
                     /locus_tag="OO249_003392"
     CDS             3318..4769
                     /gene="panF"
                     /locus_tag="OO249_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5637567.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            4781..5662
                     /gene="prmA"
                     /locus_tag="OO249_003393"
     CDS             4781..5662
                     /gene="prmA"
                     /locus_tag="OO249_003393"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5637568.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            5991..6956
                     /gene="dusB"
                     /locus_tag="OO249_003394"
     CDS             5991..6956
                     /gene="dusB"
                     /locus_tag="OO249_003394"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5637569.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            6982..7278
                     /gene="fis"
                     /locus_tag="OO249_003395"
     CDS             6982..7278
                     /gene="fis"
                     /locus_tag="OO249_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5637570.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            7364..8248
                     /gene="yhdJ"
                     /locus_tag="OO249_003396"
     CDS             7364..8248
                     /gene="yhdJ"
                     /locus_tag="OO249_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5637571.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            8332..8511
                     /locus_tag="OO249_003397"
     CDS             8332..8511
                     /locus_tag="OO249_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5637572.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(8514..9176)
                     /gene="acrS"
                     /locus_tag="OO249_003398"
     CDS             complement(8514..9176)
                     /gene="acrS"
                     /locus_tag="OO249_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR5637573.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         2419 a         2274 c         2318 g         2524 t
ORIGIN      
        1 aaatttttcc ttcactacca cagacaccaa taatacgagt tacgacttct ttcatggcgg
       61 cctttgcggg ctgcatatag tggcgtggat catttgccgc aggattgttg tagaaatatc
      121 cttttaacgc atcggcaaac gcaattttca attctgtcgc gacattcact ttacaaatac
      181 cgcggcgaat acagtgctga acgtcctgtt ctgataagcc cgaagcacca tgcaacacca
      241 gtggaatctc cactcgctga cgaatctgtt ccagccgcac aaaatccagt cgtggttcat
      301 gcgcatataa accatgtgct gtgccgattg ctacggccag ggagtcaatt tgggtacgtt
      361 gaacaaactc tgcagctgcg gctggtgagg tataagcgga atctttttca tccaccagca
      421 gatcgtcctc ctggccgcca agacgcccta gctcggcctc aacgctaaca tcaaagcgat
      481 ggcaaaaggt ggtaacttct tgcactcgcg caatgttgtc ttcaaatggc aaatgggagc
      541 catcaatcat gacagaacgg atccctgcgc gaaccttgtt aaaaatgtca tcactctctt
      601 catgatggtc gagatgcagg actaacggca ggttgtactc gcgagccagc gattgtacta
      661 acgaaattaa acttcccacg ccaccataac gataagtacc gggtgttccc gccagcataa
      721 cgggagagcg aagttgtgca gcgctctcaa caactacctg taaagtttca aggttatgaa
      781 tgttgaaagc ggggacggca tatccctctg actgggcttt tttcagcatg tttttgcttg
      841 aaatgatgta catcgcagct cctgttattt gtcgttcgcc ttacaaaatg aagaaagaga
      901 atattcactt tcattttgaa agcaaactag cacagcaaaa gcggcaaaaa gcgtgataca
      961 tgtcgaatat catcaaaata acttacaaaa aaataactta atttgttttt cactttacaa
     1021 gtgaaaccaa taacatctct gcataaatat gaataaattc atttattttt caatgaatta
     1081 atctattttt gttgcgattt ttgacccatg attttctttt cgaattgtcg atattattga
     1141 aggataactt tcaaagaaaa atacaagggg ctatagtgtt acgcattaaa ggccaatgaa
     1201 gagtgttaac gtgccgagaa aatcgtctaa cctactgaaa cgcagattaa aaattgcaga
     1261 aattgttgcc gcccaaggtg aaatcaaagt tgatgactta tctgcgcaac ttggtgtctc
     1321 tggcgtgacg atccgcggcg atctcagtta tctggagcaa cagggatatt taaagcgatc
     1381 atttggcggt gctattgcga caccactctc ctctgaacca gaagcgatac aaccgatcgc
     1441 gccattgtct ctggcacaac aaatggaaat agcacgacat tgcgcacggt taattcgcga
     1501 tcgtgacacg ctatttcttg gacatgggac aatctgccgt aagatcattc cattgctcag
     1561 cgaagtgaaa aaactgcgtc tgatcaccaa cgatcccgaa catgcgctgc tggcaaatca
     1621 gtttattgat ggcgaaataa tactggctgg cagtgaaatg ctgcgccccg acaccgcact
     1681 ggccggaaaa ccgcttgaac aggcacttca gcatttcacc attacacata gcattctgga
     1741 aatcaaccat ctggatgctg atggtacttt aaatatcaac gttcccagac tggccgctgc
     1801 ctggcagctc tgtttcgatc atgcccagaa taagacggtt atcgttgccg ccgatcctgc
     1861 gccatcaacg gctgctatcg ggcatttgca tcaggcagaa aatgtgatcg tcagccgcag
     1921 cattaacgaa caatatcagc aacaattgca gtcggctgat ttcgttatcc tttataccag
     1981 taatgagtgc tttacctggt ccaatcgcga aaggcttcag gaatgagagg ggttttgtga
     2041 caataactat ctgcgctatg ggcgaattgc tggccgagtt tttgtcccgc aacccacatc
     2101 aaaaattcac tcagcctggg gagtttatcg ggccatttcc cagcggtgcg ccagcaattt
     2161 ttgctgctca ggtggcaaaa ctgtcccatc gggccatctt cttcggatgt gttggtaatg
     2221 atgattttgg ccgactcatt atagagcgcc tccgtcatga aggtgtcatt accgatggga
     2281 tccatgttat gaacaatgcc gtcacaggta cggcgttcgt gagttatcaa aatccccagc
     2341 agcgcgattt cgtctttaat atccctaaca gcgcctgcgg cttgtttact gctgagcaca
     2401 ttgataagga tctgctaaaa cagtgtaacc atctccatat tgtgggctca tcattgttct
     2461 catttcgcat gatcgatgtc atgcgtaaag caataacgac gatcaaatcg gctggcggca
     2521 ccgtttcttt cgatcccaat attcgcaaag agatgctgag cattcctgaa atggcgcagg
     2581 ctctcgatta tttgattgaa tatacggata ttttcatccc cagcgaaagc gaactccctt
     2641 tcttcgcgcg tcacaaaaat ctgtcagaag aacagattgt tagcgatctt ctccacggcg
     2701 gcgtaaaaca tgtggcgata aaacgcgccc agcgtggggc cagctattac aagcttaaaa
     2761 acggtacatt acacgcccag catgttacag gccacgatat cgaaattatc gatccaacgg
     2821 gtgcaggcga ctgctttggc gcaacgttta tcactctttt cttatccggt ttcccggcac
     2881 ataaggcact gcaatatgca aatgccagcg gcgcgctcgc cgtaatgcgg caaggtccga
     2941 tggaagggat atcctcactg gctgacattg aagacttttt gcagcagcac tgaacggcaa
     3001 ttttctgtca gattattttc cctgcctgaa gagttgccgt agaattgccc gtttctcgta
     3061 tccccccatg aggaggaacc cttccgtgga cactcgtttt gttcaggccc ataaagaggc
     3121 gcgctgggcg ctggggctga cccttttgta tctggcagtt tggttagcag ccgcttactt
     3181 acctgacgtt gcccccggtt ttaccggctt tccgcgctgg tttgagatgg cctgcatcct
     3241 gacgccgctg ctgtttattg gactgtgctg ggcgatggtg aaatttatct atcgcgatat
     3301 cccactggag gatgacgatg cagcttgaag tgattctacc gctggtcgcc tatctggtgg
     3361 tggtgttcgg tatctctgtt tatgcgatgc gtaaacggag caccggtacc ttccttaatg
     3421 agtatttcct cggcagccgc tctatgggcg gtattgtgct ggcgatgacg ctcaccgcga
     3481 cctatatcag tgcaagttcg tttatcggcg ggcctggagc tgcttataaa tacggcctgg
     3541 gttgggtatt gctggcgatg attcagcttc ctgcagtctg gctttcgctc ggtattctcg
     3601 gcaagaagtt cgcgattctt gcgcgccgct acaatgcagt gacgctgaac gacatgctgt
     3661 ttgcccgcta ccagagtcgt cttctggtgt ggctggcgag tttgagtttg ctggttgcgt
     3721 tcgttggggc aatgaccgtg cagtttatcg gcggtgcgcg cctgctggaa accgcggcgg
     3781 gtattcctta tgaaaccggg ctgctgattt tcggtatcag cattgcgttg tataccgcct
     3841 ttggcggctt tcgcgccagc gtgctgaacg acaccatgca agggcttgtg atgctgattg
     3901 gcaccgttgt gctgcttatt ggcgtggtac atgccgcagg tggtttaagt aatgcggtag
     3961 agacgctgca aaccatcgat ccgcaactgg ttacgccaca aggcgctgac gatattctgt
     4021 cgcctgcctt tatgacgtcg ttctgggtac tggtgtgctt tggcgtgatt ggcctgccgc
     4081 atactgcggt gcgctgtatc tcttataaag acagcaaagc ggtacaccgg gggatcatca
     4141 tcggtacgat tgtagtcgca atcctgatgt tcggtatgca cctggccgga gcgttaggtc
     4201 gtgcagtgat ccccgatctc accgtaccgg atctggtgat cccgacgtta atggtaaaag
     4261 tgctgccacc gtttgctgcc gggatctttc tggctgcacc tatggctgcg atcatgtcga
     4321 caattaacgc ccaattgcta caaagttccg ctacgatcat taaagatctc tatctgaata
     4381 tccgtccgga tcaaatgcaa aacgagacgc gtctgaagcg gatgtcggcg gtaattacgt
     4441 tagttctcgg cgcgttgctg ctgcttgccg cctggaagcc gccagaaatg atcatctggc
     4501 tgaatttgct ggccttcggt gggttggaag ccgttttcct gtggccgctg gtgctgggtc
     4561 tttactggga acgcgccaac gccaaaggcg cgctaagtgc gatgatcgtt ggcggcgtgc
     4621 tgtatgccgt actcgcgacg ctgaatattc agtacctggg cttccaccct atcgtgccct
     4681 cgttactact aagtttgctg gctttcctgg tcggaaaccg tttcggtaca tccgtcccgc
     4741 aagctaccgt tttgactact gataaataaa gagttttgcc atgccttgga tccaactgaa
     4801 actgaacacc accggcgcga acgctgaaga tcttagcgat gcgctgatgg aagcgggtgc
     4861 cgtttctatc acttttcagg atacccacga tacgccagta tttgagccgc tgccgggcga
     4921 aacgcgcctg tggggcgaca ccgatgtgat tggtctgttc gacgctgaaa ccgatatgaa
     4981 cgacgtggtg gcgattctgg aaaaccatcc gctgctcggc gcaggcttcg cgcataaaat
     5041 cgaacaactg gaagataaag actgggagcg cgaatggatg gataatttcc acccgatgcg
     5101 ctttggtgaa cgactgtgga tctgcccaag ctggcgtgat gtgccggacg aaaacgccgt
     5161 caacgtgatg ttagacccag ggctggcgtt tggtacgggt acccatccaa ctacctctct
     5221 gtgcctacaa tggctcgaca gcctcgattt aactggtaaa acagtcatcg actttggctg
     5281 cggttccggc attctggcta tcgcggcgct gaaactgggt gcagcaaaag ccattggtat
     5341 tgatatcgat ccgcaggcga ttcaggctag ccgcgacaac gccgaacgta atggcgtttc
     5401 tgaccgtctg gaactctact taccgaaaga tcagccagaa gaaatgaaag ccgacgtggt
     5461 ggtcgcgaac atccttgcag gcccattacg tgaactggca ccgttaatca gcgtcctgcc
     5521 ggtttcaggc ggtttgctgg gcctttccgg tattctggca agccaggcag agagcgtttg
     5581 tgaagcttat gccgatagct tcgcactgga cccggtcgtg gaaaaagaag agtggtgccg
     5641 tattaccggt cgtaagaatt aaccttcgca tcgccgtagg gtgacgcggg gcgaagtgcg
     5701 agcaagctca caaaaggcac gtaaatttgc cggttattta cgcaaatttg cgtgccaaaa
     5761 ttttcattca taaagaaaaa ttgagaactt actcaaattt ctttgaatgt aaattttagt
     5821 cactattttc taatatgatg atttttatga gtaattatcg caccacgctc attttaaatg
     5881 caattctttg atccatctca gaggattggt caaagtttgg cctttcatct cgtgcaaaaa
     5941 atgcgtaata tacgccgcct tgcagtcaca gtatggtcat ttcttaactc atgcgcatcg
     6001 gacaatatca gctcagaaat cgcctgatcg cagcgcccat ggctggcatt acagacagac
     6061 cttttcggac gttgtgctac gagatgggag ccggattgac agtatccgag atgatgtctt
     6121 ctaacccaca ggtttgggaa agcgacaaat ctcgtttacg gatggtgcac attgatgaac
     6181 ccggtattcg caccgtgcaa attgctggta gcgatccgaa agaaatggca gatgcagcac
     6241 gtattaacgt ggaaagcggt gcccagatta ttgatatcaa tatgggttgc ccggctaaaa
     6301 aagtgaatcg caagctcgca ggttcagccc tcttgcagta cccggatgtc gttaaatcga
     6361 tccttaccga ggtcgtcaat gcagtggacg ttcctgttac cctgaagatt cgcactggct
     6421 gggcaccgga acaccgtaac tgcgaagaga ttgcccaact ggctgaagac tgtggcattc
     6481 aggctctgac cattcatggc cgtacacgcg cctgtttgtt caatggagaa gctgagtacg
     6541 acagtattcg ggcagttaag cagaaagttt ccattccggt tatcgcgaat ggcgacatta
     6601 ctgacccgct taaagccaga gctgtgctcg actatacagg ggcggatgcc ctgatgatag
     6661 gccgcgcagc tcagggaaga ccctggatct ttcgggaaat ccagcattat ctggacactg
     6721 gggagttgct gcccccactg cctttggcag aggttaagcg cttgctttgc gcgcacgttc
     6781 gggaactgca tgacttttat ggtccggcaa aagggtaccg aattgcacgt aaacacgttt
     6841 cctggtatct ccaggaacac gctccaaatg accagtttcg gcgcacattc aacgccattg
     6901 aggatgccag cgaacagctg gaggcgttgg aggcatactt cgaaaatttt gcgtaaacag
     6961 aaataaagag ctgacagaac tatgttcgaa caacgcgtaa attctgacgt actgaccgtt
     7021 tctaccgtta actctcagga tcaggtaacc caaaaacccc tgcgtgactc ggttaaacag
     7081 gcactgaaga actattttgc tcaactgaat ggtcaggatg tgaatgacct ctatgagctg
     7141 gtactggctg aagtagaaca gcccctgttg gacatggtga tgcaatacac ccgtggtaac
     7201 cagacccgtg ctgcgctgat gatgggcatc aaccgtggta cgctgcgtaa aaaattgaaa
     7261 aaatacggca tgaactaatt caggttagct aaatgcttga ttaaaaaggc gctactcggc
     7321 atggggaagc gcctttttta taggtgtcac aaagggagtg accatgagaa caggatgtga
     7381 accgacccgg tttggtaatg aagctaagac cattattcac ggtgatgcgc ttgccgaact
     7441 taaaaagatc cccgccgaaa gtgtcgatct gatctttgcc gacccaccat ataacatcgg
     7501 taaaaatttt gatggtctga tcgaagcctg gaaagaagat ctgtttatcg actggctgtt
     7561 tgaagtgatt gcagagtgcc accgcgttct gaaaaagcag ggcagcatgt acatcatgaa
     7621 cagcacggaa aacatgccct ttatcgatct ccagtgccgc aagcttttta ccatcaaaag
     7681 tcgcatcgtc tggtcatatg acagttctgg agtacaggcg aaaaaacact acggctccat
     7741 gtacgacccc atcctgatga tggtgaaaga cgcaaagaac tacacattca acggtgatgc
     7801 tattctggta gaagccaaaa ccggatcgca gcgcgcgttg atcgattatc gcaaaaatcc
     7861 tccacagcca tacaatcatc aaaaagtacc gggtaacgtc tgggattttc cgcgcgtgcg
     7921 ttatttgatg gatgaatatg agaaccaccc gacgcaaaaa ccggaagcct tactgaaacg
     7981 cattattctc gcctcttcca acccaggcga tatcgttctc gacccgtttg ccggtagctt
     8041 tactaccggt gccgtagccg tctccagcgg gcgaaaattc attggtattg agatcaacag
     8101 cgagtacatc aaaatggggc ttcgacggct ggatgtcgcg tcgcattact ctgcggaaga
     8161 actggcgaaa gtgaaaaaaa gaaagaccgg caacctgtca aaacgaagcc ggttaagcga
     8221 agttgacccc gatctcattg caaagtaaag gaagtgtaag cctggttttc agattattca
     8281 tttcgtgtat atttcctgcc agacttggtt aaacatgcac aggctcaggt aatgattcgc
     8341 aagtattggt ggctcgtcgt tttcgctgtc ttcgtttttc tgtttgatac tttactgatg
     8401 cagtggattg aactgctgac aacagaaaca gacaaatgcc gtaatatgaa ctcagttaat
     8461 ccactaaaac tggttaactg tgacgaactg aattttcagg acagaatgtg aatttacatg
     8521 acacttaatt cattcgtttg atgaattaat ttcgttatgt tttcatctgg catgaacatt
     8581 cttaatacgt tatcgaccag cgcgggtgct tgtttataaa gatcataacc cgccatattc
     8641 attaaccagt tttgaacaat tccgctgaag gcaccattaa taataatcat cacaacatct
     8701 aaatcgaggt tatttgctac acaaccttgt tgctgacacg cctgcaatac ttcgcggaga
     8761 gtctgcggat taaagcccat cttttcgcgt atcactccct cggccagcat ctcatcatta
     8821 aattcacatt tgtgatataa gattttcagc aacgcctgct ggcggggaat tttggcaata
     8881 tattgcaagc cgacaatcaa tttttcacgc aattgttgaa acgggtcatg ctctaatcca
     8941 gccgtcaagt gttcctggat taactcccgc aatgaaggct gttgcaacca catctcatta
     9001 aacagttgag tcttgttttc gaagtgccag tagatagcgc cacgcgtaac gttagcggcg
     9061 tcggcaatgt cgttgagcgt cgtcttgctt acgccatgct gcgcaaactg ggcgatggca
     9121 gtttcaatca gttcttgccg ggtcttcaga gcttcggctt tggttctttt tgccatgatt
     9181 aattattcag gaaataaata tattcgacac agggtgagaa aatagcgaag gttaatctat
     9241 catctaatgt gtatttatac gagagggtaa cattgagttg ctataaatcg tcaaataaac
     9301 aatgtatatt atttacctaa aatacattcg ccacatgaat atatatttca atttacgagg
     9361 ttttaattct gcctctttca acccgcgtca aaataaaaca gtagaatatt aatctttttt
     9421 tgtgtttatg tgccttgaga cgccagtatt cataactatt ccttacatcg acgaatgata
     9481 atttgtagga tagcaaactg tatttttctt tctgcgagtt aacgcgttgc ctttc
//
LOCUS       DAJTOR010000163         9396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTOR010000163 DAJTOR010000000
VERSION     DAJTOR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.163"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(313..891)
                     /gene="mocA"
                     /locus_tag="OO249_003399"
     CDS             complement(313..891)
                     /gene="mocA"
                     /locus_tag="OO249_003399"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCR5637574.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITVQKRYTKI"
     gene            994..1764
                     /gene="yqeC"
                     /locus_tag="OO249_003400"
     CDS             994..1764
                     /gene="yqeC"
                     /locus_tag="OO249_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCR5637575.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKM
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1812..3437
                     /locus_tag="OO249_003401"
     CDS             1812..3437
                     /locus_tag="OO249_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCR5637576.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHINRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3478..4410)
                     /gene="arcC"
                     /locus_tag="OO249_003402"
     CDS             complement(3478..4410)
                     /gene="arcC"
                     /locus_tag="OO249_003402"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5637577.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNVLREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4458..5843)
                     /gene="hyuA"
                     /locus_tag="OO249_003403"
     CDS             complement(4458..5843)
                     /gene="hyuA"
                     /locus_tag="OO249_003403"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCR5637578.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5896..7107)
                     /locus_tag="OO249_003404"
     CDS             complement(5896..7107)
                     /locus_tag="OO249_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCR5637579.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(7165..8361)
                     /gene="dpaL"
                     /locus_tag="OO249_003405"
     CDS             complement(7165..8361)
                     /gene="dpaL"
                     /locus_tag="OO249_003405"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCR5637580.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8419..>9396)
                     /gene="ygeW"
                     /locus_tag="OO249_003406"
     CDS             complement(8419..>9396)
                     /gene="ygeW"
                     /locus_tag="OO249_003406"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR5637581.1"
                     /translation="TRTRFSYASALNLLGLAQQDLDEGKSQIAHGETVRETANMISFC
                     ADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQCDIDHPTQSMADL
                     AWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFGMDVTLAHPEGYD
                     LIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKVMEERTELLRAND
                     HEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADISGVSCKEGEVTE
                     GVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQERVK"
BASE COUNT         2195 a         2292 c         2462 g         2447 t
ORIGIN      
        1 cttagcttat acagatatca ttgagtgacc ggtgttgaga aatccggtgg tttaggggga
       61 tagagcaaca atcgcaccac tcttgccagg tttggcggga aatataaaaa aatgtgaggc
      121 ggctcgaaac ttaagggctt tttgtgaatg aactcacagg tgctaaatga gaattaaaat
      181 cactcctgaa aattattatt gccagcccat atatcacttt ttctcttttc tcaaaatcag
      241 tgattcctta tcattatgat aaagagtgct ggagggatta aatatttgaa cgcaatcgtt
      301 taactacgtt ttttaaattt tagtatacct tttttgcact gtaataaagt cgtccggagt
      361 atcaatatct aaaattattt ctgcattttc tacttcgacg gaataatgag ctcccataag
      421 gagtgcctga cgcatattgg tgatattggg gcgcttgatt gcctgcatca ggcatggttt
      481 tgacactaaa atcggatggc cggggatgcc attatggagt ggcagtattg cgccatcatt
      541 tcgtaacgac cagatttttc tgaaaatatc gatggtgagg gttggcatgt caccgtgggt
      601 gagaaaacaa tgttctgttt gcactgcggg tactgcggcc tttactgatg ttagtaaacc
      661 ctgcgcataa tctgggttgt ggataatagt gatattgctc tggttcacat aacgttcgtg
      721 cagttcatta ccacgatagc cggtgactaa aataattcgg ctacaaaact gcaacgcatt
      781 tttgatactt gtatcaagaa ttgtcccctg ttgccagggt aacatcattt tccattgccc
      841 cattcttgat gataatccgg cagcggtaat tatacagtcg atggctgaca tagtggttcc
      901 attatttata ggggctaata acgcctgata attgcgttac ttcctgatgg attaagttgg
      961 ggataattta tcccagagag gtcataaaga ctcgtgaaaa gtatagttga cccatcggca
     1021 ttattcattg atttaggtgc gcagaaacgt cccactgtga tttctgttgt tggtgctggg
     1081 gggaaaacca gcttgctttt ttggctggca gaactgctcc aggctagcgg caggcgtgtg
     1141 ttaatcacga cgacaacaca tatgtttatg ccaacgtctc actggcccgt ggttttctgt
     1201 cgtgatcccg ccatgcttcc tcatgcgtct cttacatccc ccatttcatt ttgttttcac
     1261 agctggaaag cgaaccaggg aaaagtgcag ggatttacgc cagaagcgat tgatgcactg
     1321 gtgcaacgac cagagtgtga cgtaattctc attgaagcag atggctcgcg tgcaatgccg
     1381 ttaaaagcgc ctgatgagca cgaaccttgc atacccaaaa gcagttgctg cgtgattgct
     1441 gtgatgggag gacatatttt gggcgcgaaa atgagcacag aaaatgtcca tcgctggtcg
     1501 cagtttgctg atattactgg gttaacacct gatgcaacct tgcaactgag cgatctcgtt
     1561 gcgctggttc gccaccctca gggggcgttt aaaaacgtac cgcaaggttg tcggcgagtc
     1621 tggttcatta accgtttttc tcaatgtgag aatgcgattg cacaaagcga gctccttcaa
     1681 ccgctgcaac aacacgacgt agaggcaatc tggctaggcg atatacaaga acatcctgca
     1741 atcgcgcgca gatttgtgaa ttagcggccc gggtattccg ggttactgaa aggatatcca
     1801 ttgaggttgg tatgaatatt ttcacagagg ctgcaaaact cgaagagcaa aattgtccgt
     1861 ttgcgatggc gcaaattgtt gatagccgag gctcgactcc ccgccattct gcacaaatgt
     1921 tagtgcgcgc cgatggttct atcgtcggta caattggtgg cggaatggtt gagcggaagg
     1981 tgattgaaga gtcacttcag gcattgcagg aacgtaagcc gcgattattc catggacgta
     2041 tggctcgtaa cggtgcggat gctgtcgggt cagattgtgg aggtgcaatg tcagtattta
     2101 tcagcgtcca tggtatgcgt ccacgtctgg tgttgattgg cgcggggcat atcaaccggg
     2161 cgatagccca gagtgccgcg ctattaggat ttgatattgc cgttgccgat atttatcgcg
     2221 aaagcctcaa tcctgaacta ttcccaccat caaccacgct tctccatgct gagtcgtttg
     2281 gtgcggcagt ggaagcactg gatattcgcc ctgataattt tgtcctgatt gccacgaata
     2341 atcaggatcg tgaagccctc gacaaactca ttgaacagcc cattgcatgg ttggggttgc
     2401 tggcaagtcg ccgcaaggtt cagcttttcc tgcgtcaatt gcgtgagaaa ggcgtggctg
     2461 aagaacatat tgcccgttta catgcgcccg ttggttacaa cataggtgcg gaaacgccgc
     2521 aggagatcgc catcagcgtg ctggcagaaa tattacaggt gaaaaataac gcgccgggtg
     2581 ggctgatgat gaaaccttct catccttccg gacaccagct ggtggtgatt cgcggtgcgg
     2641 gagatatcgc cagtggtgtg gcgctacgtc tgtatcatgc gggttttaaa gtgatcatgc
     2701 tggaagtgga aaaaccgaca gtgattcgtt gtaccgtggc gtttgcccag gccgtgttcg
     2761 atggcgaaat gacggtcgaa ggcgtcactg ctcgcctggc aaccagctct gcggaagcga
     2821 tgaaacttac cgaacgcgga ttcatccctg tgatggtaga tcccgcctgt tcattgcttg
     2881 atgaactgaa accgctttgc gtggtggacg ctattctggc gaaacagaat ttgggaacgc
     2941 gggcagatat ggcaccagta acaatcgcgc ttgggccggg ctttactgca gggaaggatt
     3001 gtcatgcggt aattgaaaca aatcgcgggc actggctcgg tcaggtgatt tactctggtt
     3061 gtgcgcagga gaataccggt gttcctggca atattatggg gcataccacc cgacgggtga
     3121 tccgtgctcc tgctgcaggc attatgcgat ccaacgtgaa attaggcgat ctggtgaaag
     3181 agggcgatgt gattgcctgg attggtgagc atgaaattaa agcaccgttg acggggatgg
     3241 tgcgtggctt gttgaacgac ggcctggcag tggttggtgg ttttaaaatt ggtgatatcg
     3301 atcctcgtgg tgaaaccgct gatttcacca gcgtttctga taaagcccgg gcgattggcg
     3361 gcggcgtact tgaggcgtta atgatgttga tgcatcaggg cgtgaaagcg acaaaagaag
     3421 tgctggaagt ggcttaaatg aaaatggccg gatggtatta cccacccggc cttaaaatta
     3481 ctgacaaatc accgttccgg ttttgccttc aatcccttct ttcgctttgc tcaatacggt
     3541 aatcagtgct tcacgacctg cgcgagagcg agcaaacgag gcggcagctt ccactttagg
     3601 cagcatagaa cctttcgcaa aatgaccttc ttcgataaag cgttcagcat cacttaatga
     3661 caggcgatcg agccactgtt cattctcttt accgaagtta atggccactt tttctaccgc
     3721 agtcagaatg atcagcatat cggcatcaat catttctgct aaacgggcgc tggcccagtc
     3781 tttatcgata accgcgctgg caccacgcag atggttacct tcacgaataa cagggatacc
     3841 accaccgcca acggtgatca ccacctggcc tgcatctacc agagctttaa cagtttcttt
     3901 ttcaatgata tcaaccggtt ttggcgaggc aacaacacgg cgatagccgc gaccagcatc
     3961 ttctttcaga gtgtaaccct gttttgtcag ttgctctgct tcttgctcgg taaagaacga
     4021 accgatcggc ttggtcgggt tgaggaatgc cggatcgtta gcgtcgactt cgacctgagt
     4081 aaccagcgtt gctacaggtt tattaatgcc ccgagaaagc agttcttctc ttaacacgtt
     4141 ttgtagatcg taaccaatat aaccctggct taaggcaaca cagacagaca tcggcagcat
     4201 tggcgagtgt gcttcagttt ttgcggcagc ttcaaaggcc tggttaatca tgcccacctg
     4261 cggcccgtta ccatgagtga cgataacttc atgtccttgg gcaattaaat caacaattgc
     4321 ctgagaagta atttttaccg ctttcatttg tccggccagg tcatcgccca gcgcattccc
     4381 gcccagggcg agaacaattt tcttactcat ttttcctcac tcatcattct tgattgtgta
     4441 tagatccctg acagtggtta gagcacggga gggacaaacg gtttgcgtcg caggaaacga
     4501 cctcgcccgg ctttgcctgt aaaggtgccg tcacagaaaa tcgtttcacc acgggataag
     4561 gttctgacaa tcgcgccctg acaggtaaaa ccctcccatg gcgagtagtc ggcgttgtcg
     4621 tggagatggc gatgctgaat ttgttggctc tgacgtgggt cgataatcac cacgtcgcca
     4681 tcggaaccgg gcgctaataa tcctttttgc ggccacaggc caaacagcct ggcgggcatt
     4741 gcgctggtta attcaacaaa gcgttccggt gttatacgtc ccgtcatcac gccactggaa
     4801 aacagtaact gcatgcggtt ttccacaccg ggtaagccat ttgggcagcg actgaaatcg
     4861 cctttagaaa tttgcaggcg ttgagccatc gagaaggtgc agtgatcggt cgccaccacg
     4921 tcaatcgcac catcgctgat gccacaccac agtttgtcct gctcgcgtac gttacgcagc
     4981 ggtgggctaa gaatgaactt catgccatct tctgtatcgt aactgcgttc gtccaacagg
     5041 agatattgtg ggcaggtttc aacccagact ggctggtgat tcgcacgggc aagacgcaga
     5101 taatccagac ctaagccgtt agacaggtgc acgatataga gcggggcgtt accggcaatt
     5161 tgtgccaggt taatcatgcg ggcgatggct tccgcttcgc attccagagg gcgactcaag
     5221 gcatgatagc gcggcgcggt taaccctgcg gcgataaatt ccgcccgctt gctggcgata
     5281 gccgcatcat tttccgggtg cacggtggtc agcgcgccgg attcatgcaa gcggcgtaat
     5341 gcctgcaaaa cctcgtcatc gttgagtttg tattgatagg ttaaatagag tttaaaactg
     5401 ctcagtcctt cctcgaccat catcgggatt tcgtcgagga ttgcgtgatt aatgtgctgg
     5461 atcacaccgt gaaagctgta atcgatgacc gctttatggg cggcataacc acgataaacc
     5521 tccagttgat ggcgtaaccg acagccgttt gggccaaatc ccatatggtc aataatggtt
     5581 gttgtaccgc cacacgcagc tgcgcgggta ccggtaaaaa aatcatcaca actgcgcgcg
     5641 atgccgacat caatattgaa atgcgtatgg acatccacgc cgcctgggaa aacgtaacag
     5701 ccagtggcat caatttcttc atacgggagc tgcggcgaaa tattgttgcc caactggcga
     5761 acaatcccgc tttcaatcag caaatcctgt ttggcttgtc catctgcgtt aacgacagtg
     5821 ccgtttttga tcaatacgcg catagcaaac tccaaaaacc ctccggcgaa ccggagggga
     5881 ataagtgaag agtaattact ctgttgccag ccagcttaac gggattgcag cgtacatcgc
     5941 ggcacaggtc accaggtgag atttccaggt tttttcgtta ggtgcatgcg cttcaggttc
     6001 tttacccggg ccaaagccga tcaccggaat gccgtgacgg cccatgatag atacgccgtt
     6061 agttgagaag gtccacttat caacaaccgg cgctttgcca aacagccctt cgtaggcatt
     6121 caccagtgct ttaacggtga agtgatcttc ttccactttc caggtcggga agtagcattc
     6181 ggttgggtaa accaggccag tccaggacgg acggtcgtag ttgtacatag aaacaaccgc
     6241 gttagctttc tgtactgcag gcagggcgcg gatttcgtcc agcgcgcctt cccaggtttc
     6301 gccccaggtc agacggcggt caatagagac ggcgcagcta tctgctacag cgcaacggct
     6361 tggggatgtg aagaagattt cagaaacggt gagggtgcct ttgccgagga attcgtcata
     6421 acccagacgt tgggagagtt cttgtaattc gccaagaatc ggacccattt tgaaaatggc
     6481 gttgtcaccg cgttctggcg cagaaccgtg gcagctaaca ccctgaacat caatacgaat
     6541 ttccatacga ccgcgctgac cacggtatat ctggcagtcg gttggttcgg tactgaccac
     6601 aaattccggg cgaatgccgg attgttcaat aatgtactgc cagcacagac cgtcgcagtc
     6661 ttcttcctgc acagtaccgg taaccagcag ggtatattca tcttccagac cgaggtcttt
     6721 aatgatttta ccggcataaa ccatagatgc catgccgcct tcctggtcgg aagtaccgcg
     6781 accaccgatt agctcatcag tttccatgcc ttcgtacgga tcgaagtccc agtttttgat
     6841 gttgccaatg ccgacggtat cgatatgagc gtccattgcc accagacgcg ggccgtggcc
     6901 gatataaccg agaacgttgc ccatcgggtc gatttcaact ttatcgaagc cgactttttc
     6961 catctcttct ttaatacgat gtactacgcg tttttcgtcg cagctttcac tgggaatagc
     7021 aaccatgtcg cgcaggaagc gagtcatgtc cgcctggtaa tcttttgctt tttcaagaat
     7081 cagtttgaat ggaatattct tagccattat ttttctccag attttttact ccccgttttg
     7141 cagggagtag ttccaatagg gtgattaagg tgctacagcg tgtttgcctt cccagacaac
     7201 ttcgcggtag tgcttcacgt cggtgtcgcc ttcagtactg ataaccagca ctacggcatc
     7261 tttgttcagc gccagttttt ccatcaggct ttgacgttgc gggtgataat gaaccgctgc
     7321 gagaacgccc aaaccgacag cgccggattc accggagatg atgcgcgggt cgttgccgta
     7381 cggattaccc agcacgcgca tacctaatgc ggcaacgctg tcctggcagg agatgaattg
     7441 ggtggcgcag ttacgtagga tttcccagcc cagcgggtta ggttcgccac aggccaggcc
     7501 cgccatgatg gtggccatat caccgccaac gttgacgatg tcgcctttga cgccggagcg
     7561 ataaatacag tcagctttgt caggttcaac aataatgctg tgcagatttt gcgggctgta
     7621 gacgtcgacc agataaccca gcacaccacc ggccattgct ccgacaccgg cttgcagcag
     7681 aacgtgcgtc ggggttacgc ccatttcacg catttgctcg acggcttcat ctgccagggt
     7741 tgcgtagcct tgcatgatcc aggttgggat tttggtgtaa ccttcccatg ccgtgtcctg
     7801 taccacttcc cagccgtgct gctgcgcgtg ttgcatggtc aggcgaacgg tatcgtcata
     7861 gttcatatcc gtgacgatgc actcggcacc gaggttcaga atggcgtcaa cgcgttcctg
     7921 agcagaacct ttcggcatgt aaatcaccgc attctgtccg agttgctgtg ctgcccacgc
     7981 cacaccgcgc ccgtggttgc cgtcggtggt ggtcgcgaaa gtcatttttt cgccgatggc
     8041 atttttcagg tgctcaaatg acagcgtttc gatatcaaga tgatattttt cacacaataa
     8101 ttgagcgatg gcgtacgcac cgccaagcat tttgaaggcg ttcagaccga atcgttttga
     8161 ttcgtctttg acgagaattt tcttcacacc aaaaaggttt gcgagatcgt ccagcgcaca
     8221 aagcggtgtt gggcgataac cagctatttt ctggtggaac tggcgcgcca gtttggcctg
     8281 gctttgcgaa aagagcggtg atgtttcgcc gttgaaaaat ttgttatcgg cgatatcaat
     8341 cttcaatgag aaaacggaca tatcctatcc ttttggcctc tggaaataga gggccgacca
     8401 gctgatcggc ccgaggggtt atttcacgcg ttcttgcgct tctttcagca gttgctcgag
     8461 cagtgcacca ggtttggcgt atttacggga caggatcatc gcggcgatga tataaggctt
     8521 ccagctggct tctttgtagg tagcgatacg gtatttttcg aatacgcctt cagtcacttc
     8581 accttctttg caggatacgc cgctgatatc agctggcagg cagtgcatgt acagggcttc
     8641 gccatcacgg gtcagttcca tcatctcttc agtacaatgc cagtctttgt gttgcgcgtt
     8701 ctgtgccaga cactgttttt ccagtgcttt taagccttcg tggtcgttcg cacgcagcaa
     8761 ttcagtacgc tcttccatca ctttgtaagg tgcccatgac ttcggataaa cgatgtctgc
     8821 gtctttgaag gcttcttcca tgctggtgac ctgacggaag ctaccaccgg aggctttagc
     8881 attgtttttc gccacttcaa ccacatccgg gatcaggtcg tagccttccg gatgggccag
     8941 ggtgacatcc ataccgaagc gagtcatcag accgatgatg ccttgtggta cagagagcgg
     9001 tttgccatag cttggagagt aggcccaggt catggcgatt tttttacctt tcaggttttc
     9061 cagtgaacca aagtgttcac gtaaccacgc gaggtcagcc attgactgag tcgggtggtc
     9121 aatatcgcat tgcaggttca ctaaagccgg acgctgtggc agtacaccct gcttgtaacc
     9181 gtcatcaagt gcagcgccaa cttcacgcat ataggcgttg cctgcgccca gatacatatc
     9241 gtcgcgaata ccaatagcgt cggcgcagaa ggagatcata ttggcggttt cacgcacggt
     9301 ttcgccgtga gcgatttgtg atttgccttc gtcgagatct tgttgtgcaa ggccgagcag
     9361 gttaagcgcg gaagcataag agaagcgggt acgggt
//
LOCUS       DAJTOR010000164         9318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTOR010000164 DAJTOR010000000
VERSION     DAJTOR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.164"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(671..1273)
                     /locus_tag="OO249_003407"
     CDS             complement(671..1273)
                     /locus_tag="OO249_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637582.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSPEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            complement(1368..1574)
                     /locus_tag="OO249_003408"
     CDS             complement(1368..1574)
                     /locus_tag="OO249_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5637583.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            1715..1981
                     /locus_tag="OO249_003409"
     CDS             1715..1981
                     /locus_tag="OO249_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637584.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            2315..2458
                     /locus_tag="OO249_003410"
                     /pseudo
     CDS             2315..2458
                     /locus_tag="OO249_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            3203..3772
                     /locus_tag="OO249_003411"
     CDS             3203..3772
                     /locus_tag="OO249_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5637585.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            4032..4433
                     /locus_tag="OO249_003412"
     CDS             4032..4433
                     /locus_tag="OO249_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637586.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            4421..4855
                     /locus_tag="OO249_003413"
     CDS             4421..4855
                     /locus_tag="OO249_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5637587.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            5210..5590
                     /locus_tag="OO249_003414"
     CDS             5210..5590
                     /locus_tag="OO249_003414"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5637588.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            5587..5934
                     /gene="tnpB"
                     /locus_tag="OO249_003415"
     CDS             5587..5934
                     /gene="tnpB"
                     /locus_tag="OO249_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5637589.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            5984..7369
                     /locus_tag="OO249_003416"
     CDS             5984..7369
                     /locus_tag="OO249_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5637590.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(7608..8966)
                     /locus_tag="OO249_003417"
     CDS             complement(7608..8966)
                     /locus_tag="OO249_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5637591.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT         2215 a         2414 c         2527 g         2162 t
ORIGIN      
        1 ggaaaatgaa gtctgaaaag gagcacaatg aaaagaggcg tgtcccgtaa atataacgaa
       61 tcatcgcagg atatacatta tcaggtgata ccagagggca ttaaactgtc gccacggata
      121 agaatataca acatttctga taacaaaaca tcataacgtg tttcagatat gttccggtat
      181 cagtctgacg gatattattt cccggtaaaa caaaccggct actgtaacgg ttaactgtat
      241 ctgcataaat tatttatgat gaaagaacag tattacggat tcaaatatca tcctgtttgc
      301 attcaggcgg acattcccgg atgccggaaa gaatatcctg caggctgagt gagtctcttc
      361 cacagtgccg ggatcgctgt cgccacgtac atattgtcac caggatgggt atgtaccgca
      421 gcatgccatt tctggtgaat aaccgcacct gcacattcag tctgctgaga acctggcgtg
      481 tatcgggctg taaagaacat ccctgtccgt ggctcctctg gtggtgttgc ctgctcagca
      541 atgacaggat gcccttaaga cgcccgtcat ggctcagtat gatgacgacg aacacgctct
      601 gcaggtgagc atgggggccg ggagcatcat catttccgta ttttgtcaca ccttccggaa
      661 gcaaccggca tcagttgcgg ctatcgtcaa ccgttgtgcg gtatgccagg agcacgtact
      721 gatccatcag atccgtcagt gctgcgctct gcctctgacg gttgatgatc cattcctcca
      781 gctctttttc tgatttccgt ttcaggttct ctttccagtc ttcagggctg tttccggtca
      841 ttaaatcaag ccagcaaagc cagaccagtt tgaggcgcaa ctccgtgcca ccaaactgac
      901 ggaagaggct gatcagttta tttgccagag aacgctgcct cagacgttca cattcagaca
      961 ggagaatgcc ttccagcatc ggatgatgaa ccagcagatg ttcactggtg cataaattca
     1021 ctatcagatc atcatccagg ggcggcgtgg cccgtttgtc agggccataa atcagccggt
     1081 gcaggctctg gggtacgatg gcaatctccg gtttttcacc attgaatgac acccaaccgg
     1141 gcgtcagatt atggaagtag tagatattcg gatgacacac gccccaggat acggcgtgcc
     1201 gggtgaagcc ggtcagccat ttctggtagc tttcgtggag ccattcgtag cgggtctgat
     1261 actgttgttt cattttcata ttcctcacgg ggatataaca ggcagcagaa tgccgccggc
     1321 aggccgggga ttacgtctgc tgcgggtgaa cggataacgg tcagaaatta cgggcggggc
     1381 tgtgcaatac gcgcctgtag ccaggcttcc acttcacttt tcagccagcg ggagctgcgg
     1441 cccagcttga tgggagccgg aaaggcccca tccctgatga gcctgtaaaa ccacttatct
     1501 gtcaggccgg tcagttgagt gataaatgcc atgtcgacca tctggtcatc catcagtgaa
     1561 actggggtag ccatgagaat gttcctccgg atgttgttaa accagccggt gtggtcatga
     1621 aaacagcgcg ccaccttcac cggcataaca accggggaga aacgtaaaaa ataatcggga
     1681 atgtgaccgc caggagataa acggggaggt tgcggtggct gagccgtacc ggtataccgg
     1741 gtggttgttg cgaaaagatg cttccgcacg cacgcgcgat aaagagggga tagttttcgt
     1801 gaggacaggg agagaagcag gcgttctgat aattgcagag ttgttttcgg aggtagttac
     1861 agtgagtcag cccgttcctg gcgaggagga gactgatatc accgatggga taaggctgtc
     1921 tgattggtat tataaatata agaagtcatc agagagtaac gtaaaggatt tgccagagta
     1981 agctaagaga gtaaatctct ccggtaacag agcgctgtga atcgttcatg tatcagagtg
     2041 cagatacatg aacgctgatg cgtaaaatca gcagtgacac gccagctccc cctgtgccca
     2101 ttcctgtata agacatgaac tgagcgtatt aatgctcctg ccctgagact gagccggtga
     2161 gtgaggggct gagaaatccc tcttgacact gctctaaacc agaaatactt ccccggttgt
     2221 tgcggtgcgt ccggatcccc gataacggaa cactaaggct gtattgcggg acaccaggta
     2281 agtggcagcc cgtaatacgt caggagacgc gggaaaagtg aattcccaca cgcgttataa
     2341 acttaccctt gcggagctgg aagcctttaa ctctgccgtt gacaaccggc tggcagaact
     2401 gacaatgaac aaactttacg atcgcgcgcc ggcttccgtc tggaaatacg tcacctgaat
     2461 attgaagcag cgctacgatg actccggtgt tatctgcacc ggaggaccgg gaacattaag
     2521 cggtatagcg acaacaaaga attcgaaatc aaaagggaat ggtgccggag gcgtgggcgg
     2581 taataggctg attttcaggt attaccggct tacaacgtgt ggttgatatt gaggggctgt
     2641 gagtttcgcg tatttgtcag aaacccagat gagttttcct gtatgggacg aaataaggca
     2701 gcggcaatcc ctggaaaatt taatatggta tgaaggagaa cctgtcggtt acgcttaata
     2761 tacacggtac actgtcagct caggggatac atgaaccgtt atctcaccag ctacacagta
     2821 tacacacact gacgctgaca gtagtcagca ggaccacaat aatcagcgcc agcagtatgg
     2881 taacgatacg gtctgtattc cggtagtagc cgaaggggtc gacagaacca caatacgggc
     2941 aacacactgt attatgagta atcgcgtttc cacagtccct gcacttcttt ttgcgtatca
     3001 ccatcactcc ctcctcacag ccttacccgt aaccggtttt ttacattaaa aaacgacgct
     3061 gaaaattcaa ccgtcagtcc ggagacgacc gttcgggtta tcacagagtg cctgagacag
     3121 tgtcctgccg gaggtcactt cagggactct gcgtattttt tacgacgtgg ctattccgta
     3181 tgaaccatac ggagatttaa ccatgaccta caaatacaac cccttctggc agcaacgtat
     3241 tcgtgagacg gtgcggcacg cactgaatgt tcatccccgc ctgacggcat tgcgggttga
     3301 cctgcgtctc ccggatgtac cggcagcaac ggacgcagct gtgatatccc gcttcatcaa
     3361 tgccctgaaa gcccgaatcg acgcttacca gaaacgtaag catcgggaag gtaaacgcgt
     3421 gcatcccaca accctgcatt acgtctgggc ccgggagttt ggggagtgca aaggtaaaaa
     3481 acactatcac ctgatgctgc tggtcaaccg ggatacctgg tgtcgtgccg gtgattaccg
     3541 cgctccggag tctctggccg ggatgattaa acaggcgtgg tgcagtgccc tgggagtgga
     3601 tgtcgggtgc catgccacgc tggtgcattt tccggcctgg ccggcggtgt ggctggcgcg
     3661 taatgatgac accggctttc agcaagtgct ggaacgtgct gactatctgg cgaaggagca
     3721 taccaaagct cactgcaccg gtgagcgcaa ttttggctgt agccggagtt gagcacgact
     3781 ggcgcgccaa tatccggcaa accaaaagta tgggttgttg tctggcggca acgatgtatc
     3841 cactcgccca ctgactgttt tttacgtcag tgttccattg tgtgacaacc acagtgctgt
     3901 aacagcaacc agcctgcttt acccaaactc cagtccagat tttaactgaa ccgccattca
     3961 caccctgacc acgctgcccg taccgggacg gctgttgcct gcgtgtcgtc tggcggctga
     4021 ggtatatgac tatgtacgca aaatccttta tcgctcttga tggcaacgga cgtctgacgg
     4081 gcgcccgtac tgcacaggcc gcaccttatg ctaactacac ctgccacttg tgtggcagtg
     4141 cactcagata ccatccgcaa tacgaaactg aacttccctg gtttgaacac actgacgaca
     4201 ggctgacaga gcacggtcaa cagtgccctt atgtcaggcc ggagcgcaga gaaatacagt
     4261 tgattaaacg tctgcagcaa ttcgtaccgg atgccttacc cgtggtgcgt aaagccagct
     4321 ggcactgcag acaatgtcac cacgattatt atggggagca gtactgcaca cactgccaga
     4381 ccggaggatt cagtattccc cggacaactc aggaggaaat atgcgaattc tgaactgtta
     4441 tatggcgaat gacagcaaag gccattttgt tacggcgaaa gaagctgcga agcacaaccg
     4501 acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta acacttcagc ggggcaatga
     4561 cggacaacca ccgtggtttg aacatgacca gatgactgtc gctgaaaaaa tcctgctgcg
     4621 atgtacctgg cttgacccgg cagagaaaga ggcccgtcgt ttgcatctgc agggcatgac
     4681 ggttccggat tatacggtga aggtgagaaa gtggttttgt gtgatgtgtg acgaagatta
     4741 tgagggggaa aagtgctgcc cacgctgcgg taccggggta tacagcaggg cgtgggggcg
     4801 gcaggaggtg ccgtcggaag atgccagggc tgataatccg ttacagaggc tgtaatggtt
     4861 gcctccggag cagtttgcgg tgatgccttt cccgggaatg gattgccagt gcggggactg
     4921 tggagcttaa ttccggtgtt ggtggccttt ccgttgattt tatgccaaca gccccctgtg
     4981 actgacaggc tgcctcgtca ttccattcgt atctccagta acagggggtt gtattgtatt
     5041 ttattgtgcc ggttcaggtg tgcgtttccc ggcgcgtctg caccggctta accaaattca
     5101 acagggataa aatagtggta agcgtacagc ctgaaccgtc tggtcagaat ctgacgaatt
     5161 agacaaagtg gtgtccacca aataagtagt gggaaccaaa gtgtcagata tgcagaaaaa
     5221 tgtgactccc ggcaggcgaa aaggctgccc taattatcct cccgaattta aacagctgct
     5281 cgttgctgcc tcctgtgaac ccgggatatc catctcaaaa cttgctcttg aaaatggcat
     5341 taacgccaat ctgttgttca aatggcgaca acaatggcgc gagggaaagc tgctattacc
     5401 ttcttcagag agcccccagc tacttcctgt gactctcgat gcagctgccg aacagccaga
     5461 atcgctcgca gaggacccgg aaaccctcag tatcagctgt gaggtaacgt tccggcacgg
     5521 gacgctccgc ttcaatggca atgtcagcga aaagctcctg actctgctga tacaggaact
     5581 gaagcgatga tcccgttacc ttccgggacc aaaatttggc tggttgccgg tatcaccgat
     5641 atgagaaata gcttcaacgg cctggctgcg aaagtacaaa cggcgctgaa agacgatccc
     5701 atgtccggcc atattttcat tttccggggc cgcagcggca gtcaggttaa actgctgtgg
     5761 tccaccggcg acggactgtg cctcctgacc aaacggctgg agcgtgggcg cttcgcctgg
     5821 ccgtcagccc gtgatggcaa agtgttcctt acgcaggcgc agctggcgat gctgctggaa
     5881 ggtatcgact ggcgacagcc caagcggctg ctgacctccc tgaccatgct gtaaatctct
     5941 ttatcctggt tgtcacagaa taagcccggt aaaatacggg cttatgaacg acatctcttc
     6001 tgacgacatc ttcctgctga aacagcgcct ggccgaacag gaagcgctga tccacgccct
     6061 gcaggaaaag ctgagcaacc gggagcgcga aatagaccat ctgcaggcgc agctggataa
     6121 actccgccgg atgaacttcg gcagtcgttc cgaaaaagtc tcccgccgta tcgcacaaat
     6181 ggaagccgat ctgaaccggc ttcagaaaga gagcgatacg ctgactggta gggtgtatga
     6241 cccggcagta cagcgtccgt tgcgtcagac ccgcacccgt aagccgttcc ctgaatcact
     6301 accccgtgac gaaaagcgac tgttgcctgc ggcgccgtgc tgcccgaact gcggcggttc
     6361 actgagctat ctgggcgagg ataccgccga acagctggag ttgatgcgta gcgccttccg
     6421 ggttatccgg acggtacggg aaaaacatgc ctgtactcag tgcgatacca tcgtgcaggc
     6481 acctgcacct tcgcggccca tcgagcgggg tatcgccgga ccggggctgc tggcccgcgt
     6541 gctgacctcg aagtatgcag agcacacccc gctgtattgc cagtcagaaa tatacggccg
     6601 gcaaggtgtg gagctgagcc gttcactgct gtcgggctgg gtggatgcat gctgccggct
     6661 gctgtctccg ctggaagagg cgcttcatgg ctatgtcatg actgacggca aactccatgc
     6721 cgatgatacc ccggtccagg tactgctgcc gggtaataag aagacgaaga ccgggcggtt
     6781 gtgggcgtat gttcgtgatg accgcaatgc cgggtcagcg ttggcacctg cagtgtggtt
     6841 cgcttacagc ccggacagaa aaagcatcca tccgcagact catcttgctt gcttcagcgg
     6901 tgtgctgcaa gcggatgcgt acgccgggtt caacgagctg tatcgcaatg gtgggataac
     6961 ggaagctgcc tgctgggctc atgcccgccg aaagatccac gatgtgcacg tccgcatccc
     7021 gtcagcactg acggaagaag ccctggagca gatcggtcag ttgtacgcca tagaggcgga
     7081 tataagggga atgccggcag agcagcggct tgctgaacgt cagcgaaaaa cgaaaccgct
     7141 gttgaaatcc ctggaaagct ggttgcgtga aaagatgaag accctgttct tcggctctgg
     7201 ccatggtggt gagcggggag cgctactgta cagcctgatc gggacgtgca aactgaatga
     7261 cgtggatcca gaaagctacc ttcgccatgt gcttggcgtc atagcagact ggccggtcaa
     7321 ccgggtcagc gaactgcttc cgtggcgcat agcactgcca gctgaataac acatccccgt
     7381 caatacggcc ctcgctgtac gcttactttt gttcggactg cttatggcct ttttccactg
     7441 gtggcgcagg aaagttaacc ggctgccacc atgggatgac gtgtaaaccg tgcaggcatg
     7501 ttttcacgtg gtgacgcttt cattgggggt ctacgccgga accccaatat tgttcacata
     7561 catgaatttg aaaaactaag cgatactgct gtccgtacct tataaactta tttcaacgct
     7621 tccggcagcg tcacaatcca cagttcactg tcagactccg gtttctccag tggcttcact
     7681 ctgagggtcg tttcaacctg cttgtcgtca agcgtcagct ttccttccgc gttcacccga
     7741 taatccttga atgtcttgcc ctcgaccgga tgttgcatcg ccaccttcac gccaaagtcg
     7801 ttatcgttcg ccaccgccag cgttttgctg tcgatcagcg ccaacccttc agccttctcc
     7861 tgctgccagc ccaaggagcg cagatcaaca acctgcgttt tctgcgcaag ggtaatgccg
     7921 cgctgtgaca gggttttctc atcatcaaac tccggatact cacccggctt gtcgaagcct
     7981 gacaggtcgc tcgccttatt gagatcgacc tcgtaaatca ggttacgcat tctgttgttt
     8041 ttatctcttc cctgttcgat cagcaggatg tgctggttat cgagcgccac gatgtctccg
     8101 attttagcgt cgctgttttt gctgtaggcc gcgctgtcga taggataacc gtacatcgcg
     8161 gtttttccgc ttgccggatc gaagctcacc agacgcgtaa acagcgcctt tttcttgctc
     8221 ttagcgtcga tatccagcgt actttgcacg gcgacaataa tacgcccgtc cggcatacgg
     8281 gtgaggcctt caaagccgcg gttcgcctga cgccatttga ggatatttgg caaaccgccc
     8341 gcaatcgctt tttccccttc cgccgcctgc ggaccgtgga ttgccaggat tttcccttta
     8401 ctgtcgatgt taatcaggaa cgggccatac tcatcgcaca gccagtaacc tcctttacca
     8461 tccggagtga tcccttccgt atccagcccg cgattatccc ccttcagtct atgtaacgtg
     8521 tcgctgaagg ccacttcatt ggtggagcca atcacgtcgc ttgccagcgg cagaccgttg
     8581 atcgcgcctt tatcgtcatg cagaggacgg ggatcgatgg cctccgcttt gccgttttgt
     8641 acacgaatcg tcatcagcag cggcgcgaaa tctggcgtaa cgaaaatttt agtttcgttt
     8701 ttcccctctt tcggtgaatc cgcgttcgga ccgcgatcgg taatggttgc gaatgtcagg
     8761 gcatcgccct gtttaccggt gaacagcagg ccagaaccta tcccaaccgg aaggccgttc
     8821 gggaatgcac tggcaaatgc gccggcgtag ttcacgtgcg tgccttctgg aaaactgacg
     8881 acatagcgct cagccgtagg ttgtgccgcc agggcagaga aagagagcgt acaaccgatt
     8941 agcacgggaa taatttttct tttcatattg ttagcatcta taaagtgaag atcacactac
     9001 accataaaga agaaacgagt caggaaaatg acatcgaatg gcaacatgcg cgggcctgag
     9061 gccaaagaaa tgtaaaacac tgttgttatt tgtaacaacg gaaacattta cggaaattat
     9121 ttgtctttct tcgtgatcaa aaatgctgtg tcttgttgga ttggagtgat gagggctgcg
     9181 tttaggaaac gccctatatt gtacggttgc attatcgcgg taggggtaat gcacgtgtca
     9241 gtatgatgag ctgaccacac tcacgtgggt ggactggtat aacaatcgac gattgctgga
     9301 aaggcagggc catatccc
//
LOCUS       DAJTOR010000165         9176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTOR010000165 DAJTOR010000000
VERSION     DAJTOR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.165"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..67)
                     /locus_tag="OO249_003418"
     CDS             complement(<1..67)
                     /locus_tag="OO249_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5637592.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMR"
     gene            75..506
                     /gene="tnpA"
                     /locus_tag="OO249_003419"
                     /pseudo
     CDS             75..506
                     /gene="tnpA"
                     /locus_tag="OO249_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(637..1960)
                     /locus_tag="OO249_003420"
                     /pseudo
     CDS             complement(637..1960)
                     /locus_tag="OO249_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1960..3576)
                     /locus_tag="OO249_003421"
     CDS             complement(1960..3576)
                     /locus_tag="OO249_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5637593.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMIWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGGWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3872..4588
                     /locus_tag="OO249_003422"
     CDS             3872..4588
                     /locus_tag="OO249_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5637594.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4585..6246)
                     /locus_tag="OO249_003423"
     CDS             complement(4585..6246)
                     /locus_tag="OO249_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5637595.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLFWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAPALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6442..6870)
                     /gene="ibpB"
                     /locus_tag="OO249_003424"
     CDS             complement(6442..6870)
                     /gene="ibpB"
                     /locus_tag="OO249_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5637596.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6982..7395)
                     /gene="ibpA"
                     /locus_tag="OO249_003425"
     CDS             complement(6982..7395)
                     /gene="ibpA"
                     /locus_tag="OO249_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5637597.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7389..7461)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7701..8033
                     /locus_tag="OO249_003426"
     CDS             7701..8033
                     /locus_tag="OO249_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5637598.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSHVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8035..9045)
                     /locus_tag="OO249_003427"
                     /pseudo
     CDS             complement(8035..9045)
                     /locus_tag="OO249_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
BASE COUNT         2334 a         2417 c         2287 g         2138 t
ORIGIN      
        1 gcctcatttc acgctcctgt tgaccaccgg gtcttaactg gaatttaaat gcttgtagtc
       61 gtttcacagg ccaaatgata ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa
      121 ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccaaatac cgacgtcaga
      181 tttttgacta tgacgcgaca gaaaaactac gcacttactt ttcaaatgta tgtgctgatt
      241 ttgaagctga actggtttaa atggatggcg aaccagatca cgtccatttg ttaattaact
      301 atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta tcgggtaggt
      361 tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc gttttgtgga
      421 gtcctggcta ttttgccaat agctgcggag gtgcgccaat atccgtcatc cgccaataca
      481 ttgaacaaca gcaaacacct ggttaggtgg aaaaccgcgc cttatatccc cgccctgaag
      541 gacggggttt tacggcgcac cggataaatt tggttatgcg ccgtcagccg tgctaaagaa
      601 tacggaccag cacccaacgg atgccagtcc acacaattaa cgtaactctg gccagtacgc
      661 tttgttggcc tccagcaact catccagaat agctttggcg accgaggcgc tcggtaccgt
      721 tttcgataac gtaatcgcct gccacaggtg ctgatatgaa cgctgttccc aggcgtccac
      781 gaccagtttt tccaccgcca cttgctgact cattaaccct ttctgaaact gcgggatatc
      841 gccgaccacc agtggttctg gtccgttgtg cccgaccaga cacgggatct cgaccatcgc
      901 ttcatcatca aagttatgga ttgccccgtt gttaggcaca atcagcaaca tccgctcctg
      961 agtgttgaag gcaattgccg ccgccagatc gacgatgtat gacgcatgtt cgtcaatctc
     1021 cagcttggcc gccgctgaat ttcctgccgc tgtaatggcg cggcaagcat cgaaaacctg
     1081 tttctcgcga tgttccattc acctcattcg cgcgggtatg ttcagggttg gaatgctgaa
     1141 caacataatc cgggaagaga taatatttca gataggtgtt cggcaatgta tccgggtcca
     1201 gtgcctggac atcccgcgct tttgcatagg tgtcgttcca gctcgcttca gtatgtgtat
     1261 cttgctgcgg tggaacataa ccgtgcttag agacatattg ccgcagctgg ggcatcaggt
     1321 cgttgccctg caaatcttca attgatgtcc accagccaaa gtggttcagg ccgtagtagc
     1381 gcacgcgcat ctgtttgcga tcttgcagcc caacaatttg cgccatccgg ctttcaatac
     1441 cgattggcat gtcacagatg ttgaggattt tcgcattcgg gcgcagacga cgcgtggctt
     1501 ctgcgacaat tgctgccggg ttggagtagt tgagcatcca ggcatttggt gaatattttt
     1561 ccatataatc caccagttcc aggacgccgc caatggaacg catgccgtac gctattccgc
     1621 cgggtccgca agtttcctga ccaacaacgc cgtggcgcag cgggattttt tcatccagtt
     1681 cgcgcatcgg gtatttgccg acgcggatgt gcgccatgac aaaatcaacg tcgctgaatg
     1741 ccacttcagg atcggtggtg taactaaacg caatgtccgg cgctttctct ttaaggatga
     1801 ctttacaggc ttcggcaatc acttcctgcc gcgcaccatc gttatcataa aatttcagtg
     1861 cacgaagcgg gaaacggtcc tgattcgcca ggagcatcaa cacgatgcct ggcgtaaagg
     1921 tgcttccacc gcctgcgaca accactgaga atttggtcat tatactgcct ccgtaatggc
     1981 aacattttct gctgattgat gagaattaat taagctatcg agctgttcac gcagctggga
     2041 tacatgcaga ccaatgatca cctgaatggc atcgccacta cggaagacgc cgtgcgctcc
     2101 cagcttttta aagacttcgt catccagcgt ttgtgacatg tcatgcagtg caatacgtaa
     2161 acgcgtcgcg caattgttaa tgctggagat attgccgacc ccgcccaggg cttgcaggat
     2221 accggcagcc tgatccagct ctttttttgg ctctgcagcg gtggtttggc ctcgcgaggc
     2281 tttgtattcg gcttttgagt agagtttcac ttccgcatct tcacgtcccg gcgtgcacat
     2341 gttgaattgc agaatcagtg tgcggaaaac cacgaagtac agcagggtaa agcacaaccc
     2401 aatggcgatt tgggtcagca tcatatccgc gtggttgctg aacatcggga tccagttttg
     2461 cggtaaaacc tggtcaatca gacctccgcc catgttgccc accacaccaa agagatacat
     2521 cacggtcgac attgaggccg ccagcacggc gtgtaccgca aacagcaacg gtgaaatgaa
     2581 caggaaggta aattccagcg gttcggtaat tcccaccagc atggcggtta aggttgcggg
     2641 aatcagcaag cccgccactt ttacccgatt ttccggtgcg gcagtgaagt acatcgctaa
     2701 agaaatgccc acggcaccaa agatttttga gttaccgtgc agggcaaaac cgccttccgg
     2761 gaacaacgat ttcagcggct cggcgctcag actgaactct tgcagatgct gcgcccagta
     2821 catctgaatg ccgccttcaa cagctgccgg accaaagata aactgtccgt agatgaagtg
     2881 gtgtaaaccg gttgggatca gaatacgttc gaggaaggtg taaacccacc ccccaagcgc
     2941 acccgccgaa cgcaggaacg cttgcagaga ttcaatcccc atttgtactt ttggccaacc
     3001 gagcaacgtc agccaggcac aggggatcat caccaggaag gcgataatca ccacataaga
     3061 cgtcccctgg aaaatgccga gaaaaaccgg cagtttttta tcgaacagac ggttatgcag
     3121 cgccgtcaca atgccggaaa tgataattgc gccgataatg ctggtatcga gggttttaat
     3181 cccggccatc attgtcagac cgctacctgc caccgcgtcc tgagtgaaat cgacgccgaa
     3241 gtagcttccc cagatcattc ccatcgcgtt gatgaaatag ttccaggtca ggaaactcac
     3301 catcaccgcc agacaagcac gcccctgcgc ttgcttagca aggccaatgg gtaaaccgac
     3361 agcaaaaatc agcggcatat tacggaatac cgtccaaccg ccctcttcaa taatgtgtac
     3421 gatttgcgcg aataaactgt tcggatcggt cagtgattcc ccgacaaaca tcgggttttg
     3481 cagcaagatg gcaagaccca ccacaatccc ggcgaaggga aacagcagca ctggcgtgaa
     3541 catcgcgccg ccaaagcgtt gaatttgact gagcatttcc acattctctt ttctcaattc
     3601 tgtaagtagg aagcctgatt ggttatgcgg caggcctgga gcgaggatag gaagatgcga
     3661 tcggcagaac atgacgaccc aatgggattc gtgatcgctt tcatgctttt cgttgggcgg
     3721 tagagatcta cgttttcgcg aaaaagtaga catctgtgag cactggtaca cagcatcgaa
     3781 cacatcttta aaaaaaagat gttttttcaa tcgattaagc agaacttgtg ggcgcattac
     3841 ccgggcttgc aggcaaaaaa gagatctaga gatgatctac aaaagcattg cggagcggtt
     3901 aagaattcga cttaattccg cggatttcac gctaaacagc cttcttcccg gtgaaaaaaa
     3961 gttggcggaa gaatttgcag tatcccggat gaccatccgt aaagcgattg acctgctggt
     4021 agcgtggggg ctggtggtcc gccgccacgg cagcggcact tacctggtgc gcaaagatgt
     4081 gctgcatcaa accgccagcc tgaccggact ggtggaggtg ttaaaacggc agggaaaaac
     4141 ggtcaccagc caggtgctga tttttgaaat catgcctgcg cctccggcca ttgccagcca
     4201 gttacggatt caaatcaacg agcagatcta cttctcccgt cgcgttcgtt ttgtggaagg
     4261 gaaaccgctg atgctggaag acagctatat gccggttaaa ctgttccgta atctttcgct
     4321 gcaacatctg gaagggtcga agtttgaata tattgaacaa gagtgtggga ttttgattgg
     4381 cggtaattat gaaagcctga cgccggtgct cgccgataga ctgctggcgc ggcaaatgaa
     4441 ggtagcggaa cacacgccac tgctgcggat cacctcgttg tcatatagcg agagcgggga
     4501 gtttttgaat tattcagtga tgttcagaaa tgccagcgaa taccaggtgg agtaccattt
     4561 acggcgactc cacccggaaa agagttaacc gatactccag aagagcaccg ccagtaattg
     4621 gggggtgata attcgcagaa acatcactaa cggatagaca gtggcgtaag agagcgccgg
     4681 cgcaccactg gttggatgaa gattattagc aaacgccagc gccggaggat cggtcatgga
     4741 acctgccagc atcccgcaca tggtcaggta attcattttg gctaacatcc gcgccagaat
     4801 gccaacagta atcagcggaa cggcggtgat cagggcacca taaccaatcc agcttagccc
     4861 ttcgccattg accagggtat tcacaaaatc cccaccagat ttcagaccaa cgaccgagag
     4921 gaacagcacg atccccagct cccgcagcgc gaggttggca cttggcggca taaaccagaa
     4981 cagcttgccg atactgccga tacgcccgag gatcaacgcc ataatcagcg gaccgcccgc
     5041 cagccccagt ttcaacgcgg ccgggaaccc aggcacaaag acgggaatag aacctaacaa
     5101 tacgccaagc ccgatgccaa taaacactgg cagcatctga acctgttgca gtttttgctg
     5161 cgcattcccc agcacattgg caacggcatc aattgcggac ggacgcccca ccagattgag
     5221 gatatcgccg aactgcaggc tgatatcgcc actggcgacc agttcgaccc cggcacggtt
     5281 caggcgcgag ataacaacgt catagcgttc tttaaagtgc aggtcgcgaa tacgttttcc
     5341 gagcacgttt tcattggtga ccaccacacg ctcgacgcgc aaatcagtgc ctttcgtgga
     5401 cagcgaagta tcgacctcct gaccaatcac cagttgcgca ttatgtaaat ccgctggctg
     5461 ccccaccaga tgcagcaaat cgcccagttg gataatggta tctggcgaag gaacttttag
     5521 ggtttcttcg cgtttcagac gcgagcagat aattttgtcg ccgttgagaa tcggtacatc
     5581 tttaatagct aaatcatgca ggttagggtt ctcaacgcga atattgatag tcttgatcag
     5641 cgcgccgcca ttggtgcgtg aagactcgtg ctgctgagct tctgtctcga cattgacgcg
     5701 gaaaataacc cgcaacatcc acatggtgaa caaaatcccg caaatgccga atggatacgc
     5761 catcgcgtaa ctcatcccca tctgatcgac catttccatt ggtgtaccca ggtcgcgcaa
     5821 gatctgctgc cctgccccca gcgctggcgt attggtaacc gcaccggaga aaatccccag
     5881 cactaccggc agtggaatat caaacagttt atgcaggatg gcggtaacca gaccaccgat
     5941 gatgacgatc agaacagcaa acaggttgag gcgtaatccg gagacgcgca atgaggcaaa
     6001 gaagcccggc cctacttgaa tcccgatggt ataaacgaac aggatcaggc caaattcctg
     6061 aataacatgc agcatatcgc tacttaatgt catccccgcc tgagaaacaa aatggccgac
     6121 gatgatccca ccaaacagca cgccgccaat acctaatcct atgccgcgaa atttgacgtt
     6181 gccgataaac aaaccgacga ctgccaccaa agccagaata ctgaccgtta atgctatatc
     6241 actcatcgtc catttctctt gagataacat tttaattgct acggattatg gctgaatcgg
     6301 tgaagcagat ttgccttata gcgcacatta tcacgggaaa gaacgtgccg aaatatctta
     6361 aacagtcgaa atttaacgca ttgttgaaga agaaagacaa aaaaagcccc accagaatgg
     6421 cggggcaaag agaatagcta gttagctatt taacgcggga cgttcgctga tagcgatacg
     6481 ctgcgctgcg atgggttcag gctcattacg aattaaatca atatgcagta aaccgtttac
     6541 gaaggttgcg ccagagactt ccatattttc agccagcgta aagctcaggc taaatggctg
     6601 attcataagc ccttgatgca gccatttttt ctcttctttt ggctgctccg gcgtgccttt
     6661 tacgctcagg cgcgtacctt ccagttgaat ctctaaatct tcctgacgga aacctgccag
     6721 cgcaagggta atgcggtagt ggttatcgtc gcttttctca atgttgtacg gcgggaagct
     6781 ctggctttca ccggcgtttt gcagtgcgtt ggccagtttg tcaaaaccga tccattgacg
     6841 catcagtggg gataaatcga agttacgcat agtcatttct ccttctaaga agcgagtaag
     6901 tacctgcaaa tccgaagatt cgcatatgct ccctgacggc gagcatggag atgtcaggcc
     6961 gcgtcaggcg gccttaggga attagttgat ttcgatacgg cgcggttttt tcgcttccgg
     7021 aatcacgcgt tcgagatcga tatacagcaa accatttacc aggttagccc cacgaacatg
     7081 aatgttctca gctaactgga atttgcgttc aaagttgcgt tcagcgatgc cctggtacag
     7141 ataggtgcgc tctttttgtt cgtcggcgtg agcacctttc accaccagca gattatcctg
     7201 ggcggtaatt tccagttcgc tctcagcaaa accagccaca gcgatagcaa tgcggtaatg
     7261 gttttcgtct accagttcaa cgttatacgg agggtagccg ccattactct ggctctggtt
     7321 gttttctaag tggttaaaca aacggtcaaa tccaatagca gaacggtaaa gcggggataa
     7381 atcaaagtta cgcataatca atagctcctg aaatcagcga gaatgtaata ccttccacaa
     7441 tggacaggtc aggtagccag aacacccatc aggcgcgtcc tcatcggcta cgatgtaaaa
     7501 atgggtctgg aaatgaactt ttcaagcctt atcagcggac ttttttttgc agtttatggt
     7561 ctattgcata gactgagggg gcagcacgaa acgttaaagt gcgatagccg ccacagtgcg
     7621 acgtaatgat gatgttattt tcagcaaggg atcgctataa ctcatcaggc caacacccac
     7681 gatcaacaga tgaatttatt atgataagaa atgtgttgtt agcgttcatg atatgcagcg
     7741 gaatgacatt actcggagga tgctccagcg tgatgtccca taccggaggg aaagaaggaa
     7801 cgtatcccgg cacccgcgcc agcgcaacca tgattggcga cgatgaaacc aactggggca
     7861 ctaagtcgct ggcgattctc gatatgccgt ttaccgctgt gatggacacg cttttactgc
     7921 cgtgggatgt gttccgcaaa gacagctcgg tgcgctcaca cgttgaaaaa agcgaagcca
     7981 acgcgcaagc caccaatgcg gttattccac cggccagaat gcctgataac tgatttagcg
     8041 atcagtttcg gtgatccaca gctggccgcc ttccatccac gccagccatt gaccatccgg
     8101 cgagaagacc acggcgtccg cagaaggtgg attggcgtgg ttttcggtta aatactcaac
     8161 ctcgccactt tgcgcatggg cacaagcaat tcgattatcc agcacaaagc ccaaccattc
     8221 tcctgacgga tgccagttaa atgcagactg aatatccgtt ttgttatggg ttaactggcg
     8281 cggctcgccg ccctgtggcg agataagcca cagttgcaca atgccgttat catcacgcat
     8341 taaaaacgcg atttgcgtac cctgcggatt acagcgcacc cagtggcgcg ggacgttgac
     8401 taaccccgga taagcccgat ggtgggtaaa ggttaaacgt cgctgcacga cgccgcgcgg
     8461 tggcgcgggc agggtggttt ccgttccact taacggcgca tcacctgccg ctttccagcc
     8521 agcttcatct tgcggtaact caacgataaa cagctccggc actttctcgc cctttggcga
     8581 aagtgtgtcg ccaataaacg ccagcgcgtg atttcctacc catccttctt cataagcacg
     8641 attgatttca tcgctgccag gctgcggcgt gggcgtggtt ttactcacca gcacgcacca
     8701 gtggctaccg ctgtattcac gcggatgctg cttttgtacg ttgaccgggc caaacggcgc
     8761 agcaacaccg acgtttcgca aatccagcgc cggatcgagt tcatgcatta catggtcgtt
     8821 ataggtaaag ctcacccttt caccgttcgg gctaaagaca tgcacatggc tgccgccgcg
     8881 cagcgctcct ggcgtatacg gcgcggtaat atccattgca tcgagattgc tcaccttgcc
     8941 gccttcagca atgactccgc gtcgatgatg gaaatcgtaa taccatgttt catcaggatt
     9001 ttccgggcca tggatgaaaa catatttctc tgacttcgga tgaaccacgg cagcctgacg
     9061 gctgcaaatt gaaaaccgcc cctgttcgtc ctgttgttgc cgcacgctcc gcagaatatg
     9121 tatgctctga ggacctgttt tattaaggac tcatcctctt atgttgaccc gcaaaa
//
LOCUS       DAJTOR010000166         9149 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTOR010000166 DAJTOR010000000
VERSION     DAJTOR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.166"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..868
                     /gene="maeB"
                     /locus_tag="OO249_003428"
     CDS             <1..868
                     /gene="maeB"
                     /locus_tag="OO249_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5637599.1"
                     /translation="LIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYF
                     QIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSVVKNVFGY
                     RDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFGIEPRVAL
                     LSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDRMPDSPLK
                     GSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASVRRIVNMV
                     ALAVVEAQTQPL"
     gene            1160..1495
                     /gene="eutS"
                     /locus_tag="OO249_003429"
     CDS             1160..1495
                     /gene="eutS"
                     /locus_tag="OO249_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5637600.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            1508..1987
                     /gene="eutP"
                     /locus_tag="OO249_003430"
     CDS             1508..1987
                     /gene="eutP"
                     /locus_tag="OO249_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5637601.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            1962..2663
                     /gene="eutQ"
                     /locus_tag="OO249_003431"
     CDS             1962..2663
                     /gene="eutQ"
                     /locus_tag="OO249_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCR5637602.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGVLHVRHEGQTMIAKAGDVMFIPKGSSIEFGMTSS
                     VKFLYVAWPANWQSL"
     gene            2660..3469
                     /gene="eutT"
                     /locus_tag="OO249_003432"
     CDS             2660..3469
                     /gene="eutT"
                     /locus_tag="OO249_003432"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCR5637603.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQKPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVLEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGERNDY"
     gene            3459..4475
                     /gene="pta"
                     /locus_tag="OO249_003433"
     CDS             3459..4475
                     /gene="pta"
                     /locus_tag="OO249_003433"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5637604.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSFIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YNIAQRLGGYRAVGPLIQGLAVPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            4514..4807
                     /gene="eutM"
                     /locus_tag="OO249_003434"
     CDS             4514..4807
                     /gene="eutM"
                     /locus_tag="OO249_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCR5637605.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            4914..5201
                     /gene="eutN"
                     /locus_tag="OO249_003435"
     CDS             4914..5201
                     /gene="eutN"
                     /locus_tag="OO249_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCR5637606.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            5213..6616
                     /locus_tag="OO249_003436"
     CDS             5213..6616
                     /locus_tag="OO249_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCR5637607.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            6627..7463
                     /gene="eutJ"
                     /locus_tag="OO249_003437"
     CDS             6627..7463
                     /gene="eutJ"
                     /locus_tag="OO249_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCR5637608.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVAELLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            7453..9075
                     /gene="eutB"
                     /locus_tag="OO249_003438"
     CDS             7453..9075
                     /gene="eutB"
                     /locus_tag="OO249_003438"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HCR5637609.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAYEDDCVT
                     RLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSN
                     ADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAV
                     IGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAPGGLIFQS
                     ICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQV
                     TMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDC
                     CYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLNL
                     RPSPEFERWLESMGIMANGRLTKRAGDPSLFF"
BASE COUNT         2098 a         2383 c         2717 g         1951 t
ORIGIN      
        1 ccttatcggt cgtccgaacg tgatcgaaat gcgcattcag aaactgggct tgcagatcaa
       61 agcgggcgtt gattttgaga tcgtcaataa cgaatccgat ccgcgcttta aagagtactg
      121 gaccgaatac ttccagatca tgaagcgtcg cggtgtcact caggaacagg cgcagcgggc
      181 gctgatcagt aacccgacag tgatcggcgc gatcatggtt cagcgtgggg aagccgatgc
      241 aatgatctgc ggtacggtgg gcgattatca tgaacatttt agcgtggtga aaaatgtctt
      301 tggttatcgc gatggcgttc acaccgcagg tgcaatgaac gcgctgctgc tgccgagtgg
      361 taacaccttt attgccgata cctacgtcaa tgacgaacca gacgcagaag agctggcgga
      421 gatcaccttg atggcggcag aaactgtccg tcgttttggt attgagccgc gcgttgctct
      481 gttgtcgcac tccaactttg gttcttctga ctgcccgtcg tcgagcaaaa tgcgtcaggc
      541 gctggaactg gtcagggatc gtgcgccaga tctgatgatt gatggtgaaa tgcacggcga
      601 tgcagcgctg gtggaagcga ttcgcaacga ccgtatgccg gacagccctt tgaaaggttc
      661 tgccaatatt ctggtgatgc cgaacatgga agctgcccgc attagttaca acttactgcg
      721 cgtttccagc tcggaaggtg tgactgtcgg tccggtgctg atgggcgtgg cgaaaccggt
      781 tcacgtgtta acgccgatcg catcggtgcg tcgtatcgtc aacatggtgg cgctggccgt
      841 ggttgaagcg caaacccaac cgctgtaatt cttttttaac tctcacgctt accctgaata
      901 ttcagggtaa gcggtttggc tgcaatatat tagtaaagct tattactgag tttgcgaata
      961 ataaaaaagc agtctatata atatctcgat attattttat ttatattcat gcgttgcata
     1021 tgaaagttta tgcaccacag cgaatatctc tccattctta gtgatctacc tcacctttta
     1081 aacgcgcttg ccgaattttg ttatttactc tgacgaaaaa ttgtcacgat acacgaaagt
     1141 ttttcacagg cggcgactca tggataaaga acgcatcatt caggaatttg tgcccggcaa
     1201 acaggtcaca ctggcgcatc tcatcgcgca tcccggtgag gaactggcga agaaaatcgg
     1261 cgttccggat gcgggcgcaa ttggcattat gacgctaact cccggcgaaa cggcgatgat
     1321 cgccggtgat ttagcgctga aagccgctga tgtgcatatc ggttttctcg accgcttcag
     1381 cggtgcattg gtgatttatg gctcggtggg cgcagtagaa gaagcgttat cacaaaccgt
     1441 cagcggttta ggtcgcttat taaattatac gctttgtgaa atgaccaaaa gttaatacga
     1501 ggcattcatg aaacgaattg cgtttgtggg tagtgtcggg gcaggaaaaa caacgttatt
     1561 taatgcgttg caggggaatt ataccctcgc cagaaaaaca caggccgtgg aatttaatga
     1621 taagggcgat attgatacgc cgggtgaata ttttagccat ccccgctggt atcacgcctt
     1681 aattaccacg ctacaggatg tagacatgct gatctatgtc catggcgcaa atgacccgga
     1741 aagtcggttg cctgccgggt tgctggatat tggcgtcagc aaacggcaaa tcgccgtcat
     1801 cagtaaaacg gacatgccag atgctgacgt cgccgcaacg cgaaagttat tgcttgagac
     1861 gggtttcgaa gagccgattt tcgagctaaa cagccacgac ccgcaaagcg tgcagcaact
     1921 ggtggattat ctggcatctc tcaccaaaca ggaggaagca ggtgaaaaaa ctcatcacag
     1981 cgaatgatat tcgtgaagca cacgcgcgcg gcgaacaggc aatgtccgtg gttctgcgcg
     2041 ccagcattat taccccggaa gcccgtgaag tggcggatct actgggtttt accatcaccg
     2101 aatgtgatga atcgatcccg gtaacggcgt ccgtgcccgc cagcgtgcca gccgacaaaa
     2161 ccgaaagcca gcgcatccgc gaaaccatca ttgcccagct gccggaaggc cagtttaccg
     2221 aaagcctggt cgcacagctg atggaaaaag tgatgaagga aaaacagtcg ctggagcagg
     2281 gcgcaatgca gccgagcttc aaatcggtga ccggcaaagg cggcatcaaa gtgattgatg
     2341 gcagcagcgt caagtttggt cgtttcgacg gcgcagagcc gcattgcgtg ggcttgactg
     2401 atcttgtgac cggagacgac ggcagcagca tggccgccgg ttttatgcag tgggagaacg
     2461 cattcttccc gtggaccctg aactacgacg aaatcgacat ggtgctggaa ggggtgctgc
     2521 atgttcgcca cgaaggccaa accatgatcg ccaaagcagg cgacgtgatg tttatcccga
     2581 aaggctccag catcgaattt ggtatgacat ccagcgtgaa attcctgtat gtcgcctggc
     2641 cggctaactg gcaatcccta tgaaagattt catcaccgaa gcatggctaa gagcgaacca
     2701 tacgctcagc gaaggcgcag agatccatct ccctgcggac agtcgcctga cgccctctgc
     2761 ccgggagttg ctggaaagcc gccatctgcg catcaagttt attgacgagc agggccgcct
     2821 gtttgttgac gatgaacagc agaagccgca gcccgttcat gggctgacca gtagcgatga
     2881 acatccgcag gcgtgctgcg aactgtgccg ccagccggtg gcaaaaaagc cggatacgct
     2941 gactcacctg tcggcggaaa aaatggtcgc caaaagcgat ccgcgtctgg gttttcgcgc
     3001 ggttctcgac agcaccattg cgctggcagt gtggctgcaa attgaactgg cggaaccgtg
     3061 gcagccgtgg ctggcggata tccgttcgcg tcttggcaac attatgcgcg ccgatgcgct
     3121 gggggaaccg ctgggctgcc aggcgattgt cgggctttct gacgaagatc tgcatcggct
     3181 ttctcatcag ccgctgcgct atctcgacca cgatcatctg gtgctggaag ccagccacgg
     3241 gcgcgatgct gcgttgctca atctgctacg caccaaagtg cgggaaaccg aaaccgtagc
     3301 ggcgcaggtc tttatcaccc gcagctttga agtattacgc ccggacattt tgcaggcgct
     3361 gaaccgcctt tccagcacgg tctacgtgat gatgatcctg agcgtaacga agcagccgct
     3421 aacggtgaag cagatccaac aacgactggg ggaacgcaat gattattgaa cgttgtcgtg
     3481 aactggcgtt gcgagcgccc gccagagtgg tttttccgga tgcgttagat caacgtgtgc
     3541 tgaaagccgc gcaatattta catcaacaag gtctggcaac gcccattctg gtcgccaatc
     3601 cgtttgaact tcgtcagttt gcgctcagtc acggcgtggc gatggacggg ctacaggtga
     3661 ttgatccgca tggcaacctc gcaatgcggg aagaatttgc tcatcgctgg ctggcccgcg
     3721 cgggcgaaaa aacgccgccg gatgcgctgg aaaaacttac cgaccctctg atgttcgccg
     3781 cagcaatggt cagcgccggt aaagcggatg tctgtatcgc aggcaacctc tcttccacgg
     3841 caaatgtgct gcgtgccgga ttacgcatta tcggcttgca gccaggctgt aaaacgctct
     3901 ctttcatttt cctgatgttg ccacagtaca gcggcccggc gttgggcttt gccgattgca
     3961 gcgtggtgcc acagccgacg gcggcgcagc tggcggatat cgcgcttgcc agtgccgaaa
     4021 cctggcgcgc catcaccgga gaagagccgc gcgtggcgat gctgtcgttt tccagcaatg
     4081 gtagcgcccg tcacccctgt gttgctaacg tccagcaggc gacagaaatc gtccgtgagc
     4141 gcgcaccaaa gctggtggtg gatggcgagt tacagtttga cgccgccttc gtgccggaag
     4201 tggcggcgca aaaagcgcct gccagcccgc tacagggcaa ggccaatgtg atggtttttc
     4261 cgtcgctgga agccggaaat attggttaca acatcgcaca acgactcggc ggatatcgtg
     4321 ccgtcgggcc actgatacaa ggacttgccg tgccgatgca cgatctctct cgtggttgta
     4381 gcgtgcagga aattatcgag ctggcgctgg tggcagctgt gccgcgtcag acagaagtga
     4441 accgcgaaag cagtttacaa acactggttg aatgaacggt cccgttctgg accctgataa
     4501 agaggaaagc acaatggaag cattaggaat gatcgaaacc cggggcctgg ttgcactgat
     4561 tgaggcttct gacgcgatgg tgaaagctgc gcgcgtcaag ctggttggtg ttaagcagat
     4621 tggcggcggc ctgtgtactg ccatggttcg tggtgatgtt gcagcctgta aagccgcgac
     4681 cgatgcaggt gccgcagcgg cacagcgtat cggcgagctg gtatctgtac acgttatccc
     4741 gcgtccgcac ggcgacctgg aagaagtctt cccgatcggc ctgaaaggcg atagcagcaa
     4801 cctgtaatca ccctgtgtga cgtcgattcg ggcgttacgc tctctccttt ttcattttac
     4861 cttctgcgga gggtaggggg agcttttacc tgaaatacgc cacggaggcg ggtatgaaac
     4921 tggcagtcgt cactggacaa attgtttgta ccgtacgcca tcacggactg gcgcatgaca
     4981 aattgctgat ggtggaaatg attgatccac aaggtaatcc tgacgggcaa tgcgccgtcg
     5041 ccatcgacaa tattggcgcg ggaaccgggg agtgggtgtt actggtgagt ggcagttccg
     5101 cccgccaggc gcataaaagc gaaacgtcac cggtcgatct gtgcgtgatt ggcattgtcg
     5161 atgaggtggt gtctggcggt caggtaattt tccacaaata aggcagaaca tcatgaatca
     5221 acaggatatt gaacaggtgg tgaaagcggt actgctgaaa atgcaaagca gtgacacgcc
     5281 gcccgccgcc gttcatgaga tgggcgtttt cgcgtccctg gatgacgccg ttgcggcagc
     5341 caaagtcgcc cagcaagggt taaaaagcgt ggcaatgcgc cagttagcca ttgctgccat
     5401 tcgtgaggca ggcgaaaaac acgccagaga tttagcggaa cttgccgtca gtgaaaccgg
     5461 catgggccgc gttgaagata aatttgccaa aaacgtcgct caggcgcgcg gcacgccagg
     5521 cgttgagtgt ctctctccgc aggtgctgac cggcgacaac ggcctgactt taattgaaaa
     5581 cgcgccctgg ggcgtggtgg cttcggtaac tccgtccact aacccggcgg caaccgtaat
     5641 taataacgcc atcagcctga ttgccgcggg caacagcgtc atttttgccc cgcatccggc
     5701 ggcgaaaaaa gtctcccagc gggcgatcac gctgctcaac caggcgattg ttgccgcagg
     5761 cgggccggaa aacttactgg ttactgtggc aaatccggat atcgaaaccg cgcagcgttt
     5821 gttcaagttt ccgggcatcg gccttctggt ggtgaccggc ggcgaagcgg tagtagaagc
     5881 ggcgcgtaaa cacaccaata aacgtctgat tgccgcaggc gctggcaacc cgccggtagt
     5941 ggtggatgaa accgccgacc tcgcccgtgc cgctcagtcc atcgtcaaag gcgcttcttt
     6001 cgataacaac atcatttgtg ccgacgaaaa ggtactgatt gttgttgata gcgtagccga
     6061 tgaactgatg cgtctgatgg aaggccagca cgcggtgaaa ctgaccgcag aacaggcgca
     6121 gcagctgcaa ccggtgttgc tgaaaaatat cgacgagcgc ggaaaaggca ccgtcagccg
     6181 tgactgggtt ggtcgcgatg cggcgaaaat cgcggcggca atcggcctga acgtgccgca
     6241 agaaacgcgt ttgctgtttg tggaaaccac tgcagaacat ccgtttgccg tgaccgaact
     6301 gatgatgccg gtgctgcccg tcgtgcgcgt cgccaacgtg gcggatgcca ttgcgctggc
     6361 ggtgaaactg gaaggcggtt gccaccacac ggcggcaatg cactcgcgca acatcgaaaa
     6421 catgaaccag atggcgaatg ctattgatac cagcattttc gttaagaacg gaccgtgcat
     6481 tgccgggctg gggctgggcg gggaaggctg gaccaccatg accatcacca cgccaaccgg
     6541 tgaaggggta accagcgcgc gtacgtttgt ccgtctgcgt cgctgtgtat tagtcgatgc
     6601 gtttcgcatt gtttaaggag taacagatgg cgcacgacga acaatggctc accccacgtc
     6661 tgcaaacggc ggcaacgctg tgtaaccaga cgcccgccgc gacggaatct ccgctgtggc
     6721 tgggggtgga tctgggcacc tgcgatgtgg tgtcgatggt tgtcgaccgc gacggtcagc
     6781 cggtggcggt atgcctcgac tgggccgatg tggtccgcga cggcatcgtc tgggatttct
     6841 tcggcgctgt caccattgtt cgtcgccatc tcgacacgct cgagcagcaa ttcggtcgcc
     6901 gttttagcca tgcggcgact tcatttcctc caggtacgga cccgcgtatt tccatcaacg
     6961 tgctggagtc tgctggactg gaagtgagcc atgtgctgga tgagccaacc gcagttgcgg
     7021 agctgctgca actggacaac gccggtgtag tggatatcgg cggcggcact accggcattg
     7081 ccatcgtgaa aaagggcaag gtgacgtact cggcggatga agcaaccggc ggacatcaca
     7141 tctctctgac tctcgccgga aatcgccgta tttcgctgga agaggcggag cagtacaagc
     7201 gcggtcacgg tgaagagatt tggcctacgg tgaaaccggt gtacgaaaaa atggcggaca
     7261 ttgtcgcccg gcatattgaa gggcagggca ttactgattt atggctggcg ggcggctctt
     7321 gtatgcaacc gggcgtggcg gaactgtttc gcaaacaatt cccggcgtta caggtgcatt
     7381 taccgcagca cagtttgttt atgaccccgc tggcgatcgc cagtagtggg cgagagaaag
     7441 cggaggggct atatgcaaaa tgaattgcag accgcgctct ttcaggcgtt cgataccctg
     7501 aatctgcaac gggtaaaaac atttagcgtt ccaccggtga cgctttgcgg tccgggctcg
     7561 gtgagcagtt gcggacagca agcgcaaacg cgtgggctga aacatctgtt cgtgatggca
     7621 gacagctttt tgcatcaggc agggatgacc gccgggctga cgcgcagcct ggctgttaaa
     7681 ggcatcgcca tgacgctctg gccatgtccg gtgggcgaac cgtgcatcac cgacgtgtgt
     7741 gcagccgtgg cgcagttgcg tgagtcaggc tgtgatgggg tgatcgcatt tggcggcggc
     7801 tcggtgctgg atgcggcgaa agccgtggcg ttgctggtga cgaacccgga tagcacgctg
     7861 gcagagatgt cagaaaccag cgttctgcaa ccgcgcttgc cgctgattgc ctatgaagat
     7921 gactgcgtga cgcggctgat tcaggacgat gttaacgaaa cggcctacaa ccagattaaa
     7981 aactggagca tcagcgaact gcgtgagtat gtgctgagcg atgaaactag cgtggacgac
     8041 attgccttta cccgcaaagg gctgacctcg gaagtggtcg cggcggtagc gaagatttgc
     8101 tccaacgcgg acctgatcta tggcgcgaag aaaatgccgg taatcaaaaa ggccaatacc
     8161 accatcggta ttccgggcac cttcagcgcc cgtttgcagc caaatgacac tcgtgacgac
     8221 gtgcaaagta tcgccgcgca aatctacgaa gggctttcct tcggggtggg cgatgcggtg
     8281 atcggcgtca acccggtaac tgacgacgtg gaaaacttaa gccgcgtgct ggataccatt
     8341 tatggcgtga tcgacaaatt caacatccca actcagggct gcgtactggc gcacgtcacc
     8401 acccagatcg aagcgattcg tcgcggcgcg cctggcggac tgattttcca gagtatctgt
     8461 ggcagcgaaa aagggctgaa agagtttggc gtggaactgg cgatgctcga cgaagcgcgc
     8521 gcagtgggtg cagagttcaa ccgtatcgcc ggggaaaact gcctctactt cgaaaccgga
     8581 caaggctctg cgctatccgc tggcgctaac ttcggcgctg accaggtgac gatggaagca
     8641 cgtaactatg ggctggcgcg tcattacgat ccgtttatcg tcaacaccgt ggttggcttt
     8701 attgggccgg agtatctcta caacgaccgc cagattatcc gtgctggctt agaagatcac
     8761 tttatgggca agctgagcgg catctctatg ggctgtgact gctgctacac caaccacgct
     8821 gacgctgacc agaacctcaa cgaaaacctg atgatcctgc tcgccaccgc aggctgcaac
     8881 tacatcatgg ggatgccgct gggtgatgac atcatgctca actaccagac caccgcattc
     8941 cacgataccg ccactgtgcg tcagttactc aacctgcgcc cgtcaccaga gtttgaacgc
     9001 tggctggaaa gcatgggcat tatggcaaac ggtcgcctga ccaaacgggc gggcgatccg
     9061 tcactgttct tctgatgacg cggagataac acatcatgga tcaaaaacag attgaagaaa
     9121 ttgtacgcag cgtgatggcg tcaatggga
//
LOCUS       DAJTOR010000167         9089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTOR010000167 DAJTOR010000000
VERSION     DAJTOR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.167"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            52..936
                     /locus_tag="OO249_003439"
     CDS             52..936
                     /locus_tag="OO249_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5637610.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO249_003440"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO249_003440"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR5637611.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO249_003441"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO249_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5637612.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="OO249_003442"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="OO249_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5637613.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="OO249_003443"
     CDS             5160..6443
                     /locus_tag="OO249_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR5637614.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="OO249_003444"
     CDS             6590..8065
                     /locus_tag="OO249_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5637615.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPSQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATRGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIADRIRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            8159..8245
                     /locus_tag="OO249_003445"
                     /pseudo
     CDS             8159..8245
                     /locus_tag="OO249_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            8246..>9089
                     /locus_tag="OO249_003446"
     CDS             8246..>9089
                     /locus_tag="OO249_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5637616.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGREVAK
                     FSVPL"
BASE COUNT         2393 a         2181 c         2107 g         2408 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaggtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacatcatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta ccctcgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 cagtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgcct gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc acggtcaggc
     2221 caccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagttta tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgc tcaacgacac
     2581 ctacgcctgc gcccaaggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg ccgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gcctggtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg aggctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcgtttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacgga acccggcgat gagaacaacc aggacatctc
     3781 cgcgctggtt gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aattcgttga
     3901 gatgtttaaa gcaccgataa aagtgctgca tcccttgtta accgccactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tggaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct acaacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc atgaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccatatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caagcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccaa gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tagtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacatta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca ttcttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgt tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgaccagg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcaggagc tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgcgacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgccct gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggatcgt atacgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actaactgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc ggcattttat
     8281 cgccgcaagt gtcattgtgc tgacatcttc cttccttatt tttgaacttg tcgccagcga
     8341 cagagcaatg agtgcctatc tgcgctatat cgtgcagaaa gcagactcct cctttcttta
     8401 tgataagtat cagaatcaga gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca
     8461 gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt ctgccaataa
     8521 tacccatggc ttaaacctga ctgctcataa atacccgggc ttacgcggca cactacaaac
     8581 cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg cttttgatca
     8641 ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga tggccgaaga
     8701 gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg agccggttaa
     8761 tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta ttggtatcga
     8821 tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca tttaccagga
     8881 tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtgtatgttg cagggcagct
     8941 aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct tttacactca
     9001 tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg attcggggcg
     9061 tgaagtggcc aaattcagtg tgccacttc
//
LOCUS       DAJTOR010000168         8776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTOR010000168 DAJTOR010000000
VERSION     DAJTOR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.168"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            186..251
                     /gene="yobI"
                     /locus_tag="OO249_003447"
     CDS             186..251
                     /gene="yobI"
                     /locus_tag="OO249_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5637617.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            390..1001
                     /gene="ruvA"
                     /locus_tag="OO249_003448"
     CDS             390..1001
                     /gene="ruvA"
                     /locus_tag="OO249_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5637618.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            1010..2020
                     /gene="ruvB"
                     /locus_tag="OO249_003449"
     CDS             1010..2020
                     /gene="ruvB"
                     /locus_tag="OO249_003449"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5637619.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2070..2855)
                     /gene="znuB"
                     /locus_tag="OO249_003450"
     CDS             complement(2070..2855)
                     /gene="znuB"
                     /locus_tag="OO249_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5637620.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLALDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2852..3607)
                     /gene="znuC"
                     /locus_tag="OO249_003451"
     CDS             complement(2852..3607)
                     /gene="znuC"
                     /locus_tag="OO249_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5637621.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3686..4618
                     /gene="znuA"
                     /locus_tag="OO249_003452"
     CDS             3686..4618
                     /gene="znuA"
                     /locus_tag="OO249_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5637622.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4634..5956
                     /gene="mepM"
                     /locus_tag="OO249_003453"
     CDS             4634..5956
                     /gene="mepM"
                     /locus_tag="OO249_003453"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5637623.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6076..7047
                     /gene="lpxM"
                     /locus_tag="OO249_003454"
                     /gene_synonym="msbB"
     CDS             6076..7047
                     /gene="lpxM"
                     /locus_tag="OO249_003454"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5637624.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRRPGEIQPYKRKDLYPIK"
     gene            complement(7136..>8776)
                     /locus_tag="OO249_003455"
     CDS             complement(7136..>8776)
                     /locus_tag="OO249_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5637625.1"
                     /translation="IDGLPHVDFSRVDKFFTYADAFRESLPIISGELYFEAHQGCFTS
                     ESATKAHNRNMENKLHDAEFFTTITNNMTSILRSEFDEIWKEMLTLQFHDILPGSCIS
                     RVYLETEKEYIKLEAKTEKIISDTQSTLLSKIDTSSYKDPHILFNTTCFARNEWININ
                     NNWLKARVNSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSENGDLISLYDKRYGK
                     EYITENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIY
                     HYNSSYLRFAFTLTQDSPLVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVID
                     RPTHSDDSFAFAKDEIPAHHWVDLSDRETGIALIAKNKYGYRVKDQTLKLTLLRSQHK
                     PGEVVKTDNYDNFLINNFADIGKQKFIFSLFPHHGDYRVGGVINQAYIMNHPLQVVPV
                     KPHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESHGLEISAMLSWVCNYH
                     YVQYVDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2397 a         2026 c         2134 g         2219 t
ORIGIN      
        1 accttaaaat caccatcata cactaaatca gtaagttggc agcattaccg gcagcaccta
       61 tacatgtaaa gacgatatct ccaatttcat attcagcagg gtaattaata ttcacgttgt
      121 cattccattg aaatagatac gaatatacgt aatagcataa aaaaattatt ttttcaggag
      181 gtgcaatgta tatttttatt acgcatttct tcactgaata cgtaatattt aaatatttgc
      241 ttccaatata acctgtagaa taaatcatac tgcgccattt ttcagttcat cgagacacct
      301 cgcaagtttt cttcatcctt cgctggatat ctatccagca tttttttatc atacagcatt
      361 atctttgatt cattacgcag gagcgtcatg tgataggcag actcagaggc atcatcattg
      421 aaaaacaacc cccgctggtg ttaattgaag tgggcggcgt aggctatgaa gtgcatatgc
      481 cgatgacctg tttttatgaa ctccctgaag cgggtcagga agcgatcgtt ttcacccact
      541 ttgtggtgcg tgaagacgcg caactgctgt acggttttaa caataaacaa gagcgcacat
      601 tgttcaaaga gttgatcaaa accaacggcg tcggcccgaa gttggcgctg gcgatcctct
      661 ccggaatgtc agcgcagcag ttcgttaatg ccgttgaacg tgaagaagtg ggggcactgg
      721 tgaaactgcc gggtattggc aaaaaaaccg ccgaacgctt gattgttgaa atgaaagacc
      781 gatttaaagg tttgcatggc gatctgttta cccctgccgc cgacctggta ctcacatcac
      841 ctgccagccc ggcgaccgac gatgctgaac aggaagcggt tgccgcgctg gtggcgctgg
      901 gctataaacc acaagaagca agccgcatgg tgagcaaaat cgctcgccct gacgccagca
      961 gtgaaacatt aattcgcgaa gccctacgcg ccgcgttatg aggtaaagga tgattgaagc
     1021 agaccgtctg atttctgccg gtaccacttt gccggaagat gtagcagatc gcgccattcg
     1081 ccctaaatta ctggaagagt atgttggtca gccgcaggtt cgttcacaga tggagatttt
     1141 catcaaagca gcgaaactgc gcggcgatgc cctcgatcat ttgttgattt ttggtcctcc
     1201 ggggttgggt aaaactacgc ttgccaacat tgtcgccaat gaaatgggcg ttaatttacg
     1261 cacgacttct ggtccggtgc tggaaaaggc gggcgatctg gcagcgatgc tcactaacct
     1321 tgaaccgcat gacgtgctgt ttattgatga gatccaccgt ttatcgccag ttgttgaaga
     1381 agtgctgtac ccggcaatgg aagactacca actggatatc atgattggtg aaggtccggc
     1441 ggcacgctcc attaaaattg atttgccgcc gtttaccctg attggcgcaa ccacgcgcgc
     1501 aggttcgctg acatcaccgt tgcgcgaccg ttttggtatt gtgcaacgtc tggagtttta
     1561 tcaggtgccg gatctgcaat atatcgttag tcgcagtgca cgctttatgg ggcttgagat
     1621 gagtgatgac ggcgcgctgg aagttgctcg tcgcgctcgc ggtacgccac gtattgccaa
     1681 ccgtctgctg cgtcgagtgc gtgatttcgc cgaagtgaag cacgatggca ccatctcggc
     1741 agatatcgct gctcaggcgc tggacatgtt gaatgtcgat gctgaaggtt tcgattatat
     1801 ggaccgcaaa ttgttgctgg cggtaatcga taagttcttt ggtgggccgg taggtctgga
     1861 taacctggcg gcggctattg gcgaagaacg tgaaaccatt gaggatgtgc tggaacctta
     1921 tttgattcag caaggctttt tgcagcgtac accgcgtggg cgtatggcga cgacgcgggc
     1981 gtggaatcac tttggcataa cgccgccaga aatgccgtaa gtcggattgg tcgctcgcgc
     2041 cgcatccgac aaatgtgttc agcgatagat tagctggcct gctttttcat catactgata
     2101 ataaataaca gtgctgcaca tagcaccacc gacggacccg ccggcgtatc gtaaaatgcg
     2161 gaaaaggtta aaccgcccgt tacggcaacc atccccacca acacagcgac acctgccatc
     2221 tgttccggcg tgcgggcaaa gcgacgtgca gtagcagcag gaataatcag cagtgaagta
     2281 ataatcaacg cgccaacaaa tttcatcgct acaccaatcg tcaatgccgt caccagcatc
     2341 aacaacaatt tcacgcgctg taatttcaca ccatcaacaa acgccagatc cgggctaatc
     2401 gtcatcgaca gcaaattgcg ccattgccag aacaaaatag ccaccacgat gaccacgcca
     2461 atcgcaatag cgatgagatc ttctggcgtc actgccagca aatcaccgaa caggtaagcc
     2521 atcaaatcaa cacgaatgtt agacatcaga ctaacgacca ccaggcccag cgacagggca
     2581 ctgtgcgcca taatccctaa taacgtgtca agcgccagct gtggacgttt ctccagccat
     2641 accagaccgc ccgccaggag cagcgtaacg gcaattaccg catagaatgg attcacgtcc
     2701 agcaacaaac caaacgcgac gccaagtaat gaggcatgag ccagcgtatc accgaaataa
     2761 gacatacgac gccagactac aaacgaaccc agcggacccg cggcacaggc gagcatgatc
     2821 ccggctaacc aaccgggaaa taataattca atcatgagcg atcatttccc cgacgcaaaa
     2881 caattcgtcc ctgtaaatcg tgacgatgat tatgatgatg gcgatagata cccagttgtt
     2941 cagcaccacg aggaccaaac atagaaataa actccggatg tagggaaaca acttccggtg
     3001 tgccggaaca acaaatgtgg tgattcagac acagcacttc atcggttttt gccattacca
     3061 gatgcagatc gtgagagacc attaaaacgc cacaatccag ttcgcgacgc aactggtcaa
     3121 taaggtcata taacgccacc tggccattta catccacgcc ttgagtgggt tcatccagca
     3181 ctaataattg cggtcgattt aacaatgctc gcgctaacag tacacgctgt gtttcgccac
     3241 cagagagctt ttgcatcggt gcgttaatca gatgcccggc ctggacacgt ttcagtgcag
     3301 gcaaaatatc ttctttatgt gtgccagggc gtaagcgtaa aaaacggttt acggtcagtg
     3361 gcaacgtggt gtcgagatac agcttctgcg gtacatagcc gatgcgtagt tttccgttgc
     3421 gcttgataac cccttcatcg ggtgttacca gcccgagcac tacccgtacc agtgtcgact
     3481 tacctgcgcc atttggccca agtaaagtca aaatttttcc aggtttaagt tccagcgaca
     3541 catcagagag gacgcggcgt tggccaaaag aaaccgagac attttccagg gaaaccagac
     3601 ttgtcatgtt aattttagtc ttgcagtagt catgagatgt tataatatca cacttctcat
     3661 attcattacg atgattggtc gcattatgtt acataaaaaa acgcttcttt tcgcagcatt
     3721 atccgccgct ctctggggag gtgcaacaca ggccgcagat gccgccgttg tcgcttcgct
     3781 taaacccgtt gggttcatcg cttctgccat tgctgatggt gtaacagaaa cggaggtttt
     3841 actgcctgac ggcgcttcag aacatgatta ttcactgcgt ccatcggatg taaaacgctt
     3901 acagaacgcg gacttagtcg tttgggttgg cccggagatg gaagcgttta tgcaaaaacc
     3961 ggtaagcaaa ttactaggag cgaagcaggt aacgattgcg cagcttgaag atgtgaaact
     4021 gctactgatg aaaagtattc acggcgatga tgatgatcac gaccacgcgg aaaaaagtga
     4081 agaagatcac catcacggcg atttcaacat gcatctttgg ctttccccag agatagcgcg
     4141 ggctacagcg gttgcaatcc atggaaaatt agtggaactt atgccgcaaa gtcgagccaa
     4201 acttgacgcc aacctgaagg attttgaggc acaattagcc tcaaccgaaa cgcaggttgg
     4261 taacgagctc gcgccgctca aggggaaagg ttatttcgtt tttcacgatg cttacggcta
     4321 tttcgaaaaa cagttcggac tgacaccgct tggtcatttt accgttaacc ctgagattca
     4381 acctggcgcg cagcgtttac atgaaataag aacacagttg gttgagcaaa aagcaacctg
     4441 cgtttttgct gagccacagt tcaggccagc ggtcgttgag agcgtcgcac gagggacatc
     4501 cgttcgtatg ggaacgttgg atcccttggg gacgaatatc aaactgggta aaacaagcta
     4561 ttcagaattc ctgagtcaat tagccaacca gtatgcgagc tgcctgaaag gagattaatg
     4621 aggaagtgat tacgtgcaac agatagcccg ctctgtcgcc ctggcgttta ataatttacc
     4681 gcgaccacac cgcgttatgt tggggtcgct caccgttctt actctggccg tcgctgtctg
     4741 gcggccctat gtttatcacc gcgatgccac gccaattgtc aaaaccattg agctggaaca
     4801 gaacgaaatt cgttcgctct tacctgaagc cagtgagccg attgatcaag ctgcacaaga
     4861 agatgaagcc attccccagg acgaactgga tgacaaaatc gccggtgaag cgggcgtgca
     4921 tgaatatgtt gtttccactg gcgatacgct aagcagcatt ctcaatcagt atggtattga
     4981 tatgggtgat atcacccaac tggctgcggc cgacaaagaa ttgcgtaacc tgaaaatcgg
     5041 tcaacaactc tcctggacat taaccgcgga cggcgaactg cagcgcctca cctgggaagt
     5101 gtctcgtcgt gaaacccgaa cctatgaccg tactgccgct aacggtttta aaatgaccag
     5161 cgaaatgcag caaggagagt gggttaacaa tctgctgaaa ggtaccgtcg gagggagctt
     5221 tgttgccagc gccagaaacg ccggtttaac cagcgccgaa gtgagcgcag tgattaaagc
     5281 catgcagtgg caaatggatt tccgcaaact gaaaaaaggc gatgaatttg cggtgttaat
     5341 gtctcgagag atgcttgatg gtaaacgtga gcaaagccag ctgctgggcg tacgtttgcg
     5401 ttcagaaggt aaagattatt acgcaatccg cgctgaggat ggcaaattct acgaccgtaa
     5461 cggtactggt ctggcgaaag gattcttgcg attcccaacg gcgaaacagt tccgtatctc
     5521 gtctaacttt aacccgcgtc gtactaatcc ggtgaccggt cgcgttgcac cacacagagg
     5581 tgttgatttc gccatgccgc agggtacgcc agtgctttca gtgggtgacg gtgaagtggt
     5641 ggttgccaaa cgcagtggcg cagcaggtta ttatgtggct attcgtcatg gtcgcagcta
     5701 caccacgcgt tatatgcact tgcgcaagat tctggtgaaa ccgggacaga aggtgaaacg
     5761 tggcgaccgt atcgcgcttt ccggtaatac cggacgttca accgggccgc atctgcacta
     5821 tgaagtatgg ataaaccagc aggccgtaaa cccgctgacg gcaaaactgc cgcgtaccga
     5881 agggctgacc ggctccgatc gtcgcgaatt cctggcgcag gccaaagaga ttgtgccgca
     5941 gctacggttt gattaattaa catccattcg cagccggtac gcagtcagta ccggcttttt
     6001 ttatttggtg cggggcaagt tgcgccgcta cactatcacc agattgattt ttgccttatc
     6061 cgaaactgga aaagcatgga aacgaaaaaa aataatagcg aatacattcc tgagtttgat
     6121 aaatcctttc gccacccgcg ctactgggga gcatggctgg gcgtagcagc gatggtgggt
     6181 atcgctttaa cgccgccaaa gttccgtgat cccattctgg cacggctggg acgttttgcc
     6241 ggacgactgg gaaaaagctc acgccgtcgt gcgttaatca atctgtcgct ctgctttcca
     6301 gaacgtagtg aagctgaacg cgaagcgatt gttgatgaga tgtttgccac cgcgccgcaa
     6361 gcgatggcaa tgatggctga gttggcaata cgcggaccgg agaaaattca gccgcgcgtt
     6421 gactggcaag ggctgcagat catcgaagag atgaggcgta ataacgagaa agttatcttt
     6481 ctggtgccgc acggttgggc cgtcgatatt cctgccatgc tgatggcctc gcaagggcag
     6541 aaaatggcag cgatgttcca taatcagggc aacccggttt ttgattatgt ctggaacacg
     6601 gtgcgtcgtc gctttggcgg tcgtctgcat gcgagaaatg acggtattaa accattcatc
     6661 cagtcggtac gtcaggggta ctggggatat tatttacccg atcaggatca tggcccagag
     6721 cacagcgaat ttgtggattt ctttgccacc tataaagcga cgttgcccgc gattggtcgt
     6781 ttgatgaaag tgtgccgtgc gcgcgttgta ccgctgtttc cgatttatga tggcaagacg
     6841 catcgtctga cgattcaggt gcgcccaccg atggatgatc tgttagaggc ggatgatcat
     6901 acgattgcgc ggcggatgaa tgaagaagtc gagatttttg ttggtccgcg accagaacaa
     6961 tacacctgga tactaaaatt gctgaaaact cgcagaccgg gcgaaatcca accgtataag
     7021 cgtaaagatc tttatcctat caaataaaaa aaagcctctc aaattattga gaggctgtac
     7081 tcgataaaaa atctcaatga gtgttcccac tattttgagt ttttaggtta gtacgctatt
     7141 gagttaatcg tagcgtgata atttcaaaag gtttgaactc aagattgcaa tattgattgc
     7201 aataaaaact gtcatctaac ttattttctt gaagatcgac atattgaacg tagtgataat
     7261 tacataccca cgaaagcatg gctgaaattt ctaatccatg actctcatat aatcttacga
     7321 ttataccgtc gccgtcttca gctaatttta ttgtttcgat gataacgctg ttagtatcga
     7381 tagcaaaaaa tgatagtgaa gggggtaatt cacccgggtg cggtttgaca gggacgacct
     7441 gtaagggatg attcattata taggcttgat taataacacc acccacacgg taatctccat
     7501 gatgaggaaa taaggagaat atgaattttt gtttaccaat atctgcaaaa ttattaatca
     7561 ggaaattatc ataattgtct gtcttaacaa cttccccagg tttatgctgc gaacgcagca
     7621 aagttaattt aagcgtctgg tctttgacac gatagccata tttgtttttt gctattaaag
     7681 ctatgccagt ttctcgatca gaaaggtcga cccagtgatg tgctggtatt tcatctttgg
     7741 caaaagcgaa cgaatcatcg ctatgcgttg gtctgtcaat tacaccgaac tgaatttgac
     7801 attgtgcaag agatgtttgt actgacaccg ggaatttgac tttgagagaa acattaggtt
     7861 cgtgccaatc aataaaagtt tggacatcaa cgagtggact gtcctgtgtc agggtaaatg
     7921 cgaagcgaag ataagaggaa ttgtagtgat aaatcagcgt catcgtagtt ttaggcccgc
     7981 tgattacagt tgtcattttt tcagcgagta aaacatatga ttccccatca cgataatttg
     8041 aagcaaaatc ccaggccgcg aaaaaacctg catcttcgtg gtatgcacgg atctcactgt
     8101 gcatattttc agtaatgtat tctttaccat atcttttatc atatagcgaa attaaatcac
     8161 cgttttctga aaaaagaagt ttaatgtaat tattttcaat acttcgagat tctgccttca
     8221 gaccatttac gatcttattc tttggatcaa tcacagcata accataagag tttactcgtg
     8281 ctttgagcca gttgttgttt atattgatcc attcgttacg agcaaaacag gtggtattaa
     8341 ataaaatgtg cggatcttta taagaagaag tatcaatttt acttaataaa gtagactgcg
     8401 tatcagaaat aattttctcg gtttttgctt ctagtttaat atattccttt tcagtttcaa
     8461 gataaactct tgaaatacat gatccgggaa ggatgtcatg gaattgcaag gtcagcatct
     8521 ctttccatat ttcatcaaac tcactgcgaa gaattgatgt catattatta gtaatagttg
     8581 taaaaaattc agcatcatgt aatttatttt ccatatttcg attatgagct ttagttgctg
     8641 actctgatgt aaagcaccct tgatgagcct cgaaataaag ttcacctgaa ataatgggca
     8701 agctttcgcg aaaagcgtcg gcgtaagtga agaacttatc aacacggctg aaatcaacat
     8761 gaggtaagcc atcaat
//
LOCUS       DAJTOR010000169         8755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTOR010000169 DAJTOR010000000
VERSION     DAJTOR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.169"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..760
                     /gene="ppsR"
                     /locus_tag="OO249_003456"
     CDS             <1..760
                     /gene="ppsR"
                     /locus_tag="OO249_003456"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5637626.1"
                     /translation="AVMSQFPVTISSITLPFVENESRARAVKDQIDAIYHQTGVRPLV
                     FYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQEMKLDPTPIAHRTHGLNPNNLNKYD
                     ARIAAIDYTLAHDDGISLRNLDQAQVILLGVSRCGKTPTSLYLAMQFGIRTANYPFIA
                     DDMDNLVLPASLKPLQHKLFGLTIDPERLAAIREERRENSRYASLRQCRMEVAEVEAL
                     YRKNQIPWINSTNYSVEEIATKILDIMGLSRRMY"
     gene            916..1962
                     /gene="aroH"
                     /locus_tag="OO249_003457"
     CDS             916..1962
                     /gene="aroH"
                     /locus_tag="OO249_003457"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5637627.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARTSHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            2094..2285
                     /gene="hemP"
                     /locus_tag="OO249_003458"
     CDS             2094..2285
                     /gene="hemP"
                     /locus_tag="OO249_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5637628.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2289..3725)
                     /locus_tag="OO249_003459"
     CDS             complement(2289..3725)
                     /locus_tag="OO249_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5637629.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNIELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLIQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(3788..4501)
                     /gene="ydiV"
                     /locus_tag="OO249_003460"
                     /pseudo
     CDS             complement(3788..4501)
                     /gene="ydiV"
                     /locus_tag="OO249_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            complement(4747..5211)
                     /locus_tag="OO249_003461"
     CDS             complement(4747..5211)
                     /locus_tag="OO249_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5637630.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(5289..6038)
                     /gene="btuD"
                     /locus_tag="OO249_003462"
     CDS             complement(5289..6038)
                     /gene="btuD"
                     /locus_tag="OO249_003462"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5637631.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            complement(6038..6589)
                     /gene="btuE"
                     /locus_tag="OO249_003463"
     CDS             complement(6038..6589)
                     /gene="btuE"
                     /locus_tag="OO249_003463"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5637632.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(6652..7632)
                     /gene="btuC"
                     /locus_tag="OO249_003464"
     CDS             complement(6652..7632)
                     /gene="btuC"
                     /locus_tag="OO249_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5637633.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISLGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELLIGVVTATLGAPVFIWLLLKARR"
     gene            complement(7841..8170)
                     /locus_tag="OO249_003465"
     CDS             complement(7841..8170)
                     /locus_tag="OO249_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637634.1"
                     /translation="MNDDLERRVSCLESDVTEIKNNLITLTTRSESFATKSDVLEIRE
                     GLRLEMAESRQSLKSEMADLRQSLKVEMAEHRTELQKSFANQTWLLTGIVLSAMAVLV
                     AVVTVIK"
     gene            complement(8278..8640)
                     /locus_tag="OO249_003466"
     CDS             complement(8278..8640)
                     /locus_tag="OO249_003466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637635.1"
                     /translation="MKFHLDELKRMATLDNFARFLNESSTNEKCLSCGDTDMYMYLTN
                     IVEVGPEPKTAEECDLGTFVMLDYIGPFTGYPGYEGHDRENIHNYEFRLTCNRCGFVH
                     RYSARAFMNWVGKQGDEK"
     gene            complement(8643..>8755)
                     /locus_tag="OO249_003467"
     CDS             complement(8643..>8755)
                     /locus_tag="OO249_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5637636.1"
                     /translation="LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGA"
BASE COUNT         2300 a         2340 c         1999 g         2116 t
ORIGIN      
        1 cgcagtaatg tcacaatttc ccgtcactat cagcagcatt acgctgccgt ttgtcgaaaa
       61 tgagagccgt gcacgggcag tgaaggatca gattgacgca atttatcacc agacaggtgt
      121 gcgcccgctg gtcttctact ccatcgtgtt gccggagatt cgcgccatca tcttgcaaag
      181 tgaaggcttt tgccaggata tcgttcaggc gctggttgcc ccgctacaac aagagatgaa
      241 actggatcca acgccgattg ctcatcgtac ccatggcctt aaccctaata atctcaataa
      301 atatgatgcg cgcattgcgg cgattgatta cacccttgcc cacgatgacg gcatttcgtt
      361 gcgcaatctg gatcaggctc aggtgatcct gctcggtgtt tctcgctgtg gtaaaacccc
      421 caccagtctg tatctggcaa tgcaatttgg tatccgcacg gcaaactacc cctttattgc
      481 cgacgatatg gataatctgg tgctacccgc gtcgctcaaa ccgcttcagc ataaattgtt
      541 cggcctgact atcgacccgg aacgtctggc ggcgattcgc gaggaacgtc gggagaacag
      601 tcgctatgcc tcgcttcgtc agtgcagaat ggaagtcgcg gaagtggaag ccttgtaccg
      661 taaaaatcag atcccgtgga ttaacagtac caattattcg gtagaagaga ttgccaccaa
      721 gatcctcgat atcatgggcc ttagtcgccg aatgtactag agaactagtg cgctggtttg
      781 ttttttgtta tcatgctaac cacccggcga ggtgtgacac acctcgcact tgaaatcagc
      841 tgcgattggt ttatcgtgat gcgcatcact tcccggtagt cctgccgtag aagcaacaaa
      901 tttctgagac ttgtaatgaa cagaactgac gaactccgta ctgcgcgtat tgagagcctg
      961 gtaacgcccg ccgaactcgc gctacggtat cccgtaacgc ctggcgtcgc cacccatgtc
     1021 accgactccc gccgcagaat tgaaaaaata ctgaatggtg aagataagcg actgttggtc
     1081 attattggcc cctgctcgat ccacgatctc accgctgcaa tggagtacgc cacccgtctg
     1141 cagtcgctgc gcaaccagta ccagtcacgg ctggaaatcg taatgcgcac ctattttgaa
     1201 aaaccacgaa ctgttgtcgg ctggaaaggg ctaatctccg atccggattt aaacggcagc
     1261 tatcgggtaa atcacggtct ggagctggcg cgcaaattac ttttacaggt aaatgagctg
     1321 ggtgtcccta ccgcgactga gtttctcgat atggtgaccg gtcagtttat tgctgattta
     1381 atcagttggg gcgcgattgg cgctcgtact accgaaagtc agatccaccg cgaaatggct
     1441 tcagcactct cctgtccggt aggttttaaa aatggtaccg atggcaacac gcggattgcc
     1501 gtggatgcta tccgcgcagc ccgcaccagc catatgttcc tctcgccaga caaaaatggt
     1561 cagatgacca tctatcagac cagcggcaac ccgtatggcc acattattat gcgtggcggc
     1621 aaaaaaccga attatcatgc cgatgatatc gccgcagcct gcgatacgct gcacgagttt
     1681 gatttacctg aacatctggt ggtggatttc agccacggta actgccagaa gcagcaccgt
     1741 cgccagttag aagtttgtga ggatatttgt cagcaaatcc gcaatggctc tacggcgatt
     1801 gctggaatta tggcggaaag tttcctgcgc gaaggaacgc aaaaaatcgt cggcggtcag
     1861 ccgctcactt acggtcaatc cattaccgac ccgtgtctgg gctgggaaga taccgaacgc
     1921 ctggtcgaaa aactcgcctc tgcggtagat acccgcttct gaatgcgtgc ccatgcctga
     1981 tgggatgggc atttctgcgc aacttgttgt cttctcaaca cattactgct tgctctggcc
     2041 tgccataata ttgataataa gaatcattgt tatatcaatt attattaatt tttatgcgtt
     2101 atacggatag cagaaaactc acgcctgaaa cggatgccaa tcacaagacc gcttccccgc
     2161 agcccatccg gcgaattacc agccagacac tgttaggtcc ggatggcaaa ctgattatcg
     2221 atcatgacgg gcaagaatat ctgctccgta aaactcaggc tggcaagctg ctgttaacta
     2281 agtagccttt aactcgagca gctgacttcc agccgtttac cccagtcagg tggacgactg
     2341 acatagtcat catctctgtc gctgaaagga tttcgcaacg cctcatgcag gcggtgcaat
     2401 tccatcatat cacccttttc tgccgcttca atcgcccgtt gcgccaacca gttgcgcaat
     2461 accagagctg ggttaacgct ttgtatcagt tgctgacgct cactatcgct aacctcgtct
     2521 tgttgcaaac gcccccgata acgggcaaac cagtcatcaa atgccgcacg atcaataaac
     2581 tcatcacgta gcggtgacgc cgcgctgtgc tgctcggtca gactcagcat gcggaatgtg
     2641 cgggtataat cgctgcgctc tcgcgccatc agactgaata attcattcag tagcgcgtta
     2701 tcctcttttt gctccgtcat gaagcccagt ttctgccgca tccgttgtcc ataatgcgtc
     2761 aacaaaacct gctgatagct gtccagggcc tcattcaggg catctacggc aacaaatggt
     2821 gacaatgtct gcgccagacg ctgtaaattc cacaacgcga cagcaggttg attatcaaag
     2881 ctataacgcc cttgatgatc cgagtgatta caaataaaac cgggttcgta atcatcaaga
     2941 aaaccaaacg gcccgtaatc aagcgtcagt cccagcagcg acatgttgtc ggtattcatc
     3001 accccatgtg caaagccgac cgtctgccat tgggcaatta acgacgcggt acgtgcgaca
     3061 acatcgctaa accagagacg gtatttgtcc tcatcatctt caagatgtga ccagtaatga
     3121 cgaatagcaa agtcagctaa ctgacgaacc ttttccgact cgcggcggta gtaaaaatgt
     3181 tcgaaatgac caaagcgcag atgacttggt gccacacgca tcagcatcgc gcctggctcc
     3241 gccgtttccc gatacactgg cgaatcgctg gtgacgatac ttaacgcgcg ggtcgtcgga
     3301 atgcccagat aatgcatcgc ctcactggcg agactttctc gtatcgtcga acgtaaaacc
     3361 gcccgtccat cacccattcg cgaataaggc gtcaggccag cacctttcag atgccagtcc
     3421 attgtagtgc catcagcaag cagttgttcg ccgagtaaaa tgccgcgccc atcacccagt
     3481 tggcccgccc agacgccgaa ctgatgacca ctgtaaacct gcgccagtgg tgacatacca
     3541 ggcagtaggg cttcgccgcc ccagacacct gcgccatttt taaacagcga cgatggaata
     3601 ctcagcgtgt tagccagttc gatattatgc caaatcagcc gggcattatt taaaggcgta
     3661 ggggaaagtg ctgtataggt ttctggcaat tcatcgcgcc agcgggtaac aaaagacagg
     3721 gtcatagatc ctcctgtttg atagtgtaga cggttactct cgtcttaaac accagcaaac
     3781 agaagggtta tcgctgaacc aacgtcgtta tctgatttat gggaacggca ggccacaggc
     3841 atccctggag agcatggaaa tcgaaggggg taatctgtgc caatatttct gccgtatcaa
     3901 tgcctcccgc aatgatgcaa ttgcaacaag gggaaatttg cgcctagatc gcacgaataa
     3961 acggttcgaa ggagcggtgc gtgatttgtt gctgaataaa acttttatcc agcatgacgc
     4021 gggtgaaaag accatcaaaa accgctttca ttgtgctatt gcctgctccc aaattaccta
     4081 acacaagggg atacacttgc gataaggata aaagaccgcg attatctttt ccttcattga
     4141 gatgcgggta attctcatta atcaacagtt caataaacgg atattttaat agttcgcctg
     4201 cgtaattatc cctctctaat aataatgtag ccacttgtgg cgttaaatta agccaggcaa
     4261 agagtttatg ttgaataaaa aaatgttggc atgattttag taattccaac tgctctgaaa
     4321 ataattgcca atgttgctcc tcggtaagtt gcgctataac ccgactcgtc gggatacgca
     4381 ccgtgccatc ttcactggag aaatgggtaa ttaactcaac gccgacaagg acttgctggt
     4441 tatctcttat cggcaggaaa tagcaatcag aatgataaag attctccaaa aaaatcttca
     4501 tggtaaccgt ccctctcgaa ggatgttttt cagtatccgg ctaaaaggga taaggccata
     4561 agacataaca attacagaag gagtaacttt catttgttcc atgttaacca ctttttcagg
     4621 ggtctttttt tagattatcc tgattataaa cgaataatcc atttacggaa tttttgtctg
     4681 caaaatacta ctgtattcag gagtaaatgg acggcgatgt atcatcgccg tccatttttc
     4741 gacatactag attcgtctcg cctgccagaa atttttctgc caatagacat tatcaagtga
     4801 ggaacgcatc actcccttgc tggtagaggc gtggataaat tggttgttgg tatcgtaaat
     4861 acctacatgc aaaccatttt gtccggaccc cgttttgaaa aagaccaggt cacctggcag
     4921 caacttgtct ttatcaattt gcgtgccaat agaggcttgt tgtttggttt cacggggcag
     4981 ctgcaaatcg aaacgatcgc gcatcgtcac aaccacaaat cccgaacagt ccacaccgcg
     5041 ccgcgtcatg ccaccataac gatacggcgt gccatgccag ctttgtagct ggtcgttcaa
     5101 accggcaata acggtaatcg aatcagaaag tctggcattt ggcggcggtg ctttatggtg
     5161 gctacacccg gccagaaaca gtgctgtgat caaaataagg cagaaacgca ttccgtacgg
     5221 ttcctctgtt ttttattctt gcattaattt agcgtcgtaa ttacccgatt ttcaagatac
     5281 taatgaaatc agatggtcaa aatcagcatt ctgtgacctt cgatatccag acggcgaaaa
     5341 ttcatcccat aggcctgcgc cagatttggc ggcgtgagca cctcttctct gcgtccactg
     5401 gccagcattt ttccaccttt tagcaaccac gcccgatgcg catgacgcaa cgtgtggttg
     5461 agatcgtgac tgctcatcac aatcgccagt ccttgctgac acagcgcgct cagaattttg
     5521 tctaacgcac tttgttgcgc aacatcaaga ctgttcatcg gctcatcaag aagcagcaat
     5581 tggcctgcgg gattggcttg tggtgtgatt tgcaacacca ccgcagcaag acgtacgcgt
     5641 tgccattcac cgccggaaag ttgattggtg ctacgaccga gtttgtcatc aagagccagc
     5701 gcccctgcga catcattcag tagttcggta cgcgttttat cgtgctgatg cagtgtcagg
     5761 tagtgccaga ccggcattgc aaacggcggc gtctgctgtt gtgaaagata ggcgcgatgc
     5821 agcgcgagtt ttgttgcgga ccatgcttcc aggggttgcc ccgcgaactg aatgcttccc
     5881 ttaccgctgg tcattccggc cattcgtgca agcaaggtac tcttacccgc gccattcggc
     5941 cccaccaggt gcaggatctc cccagcccga acctcgccag aaagtggccc caggcgggta
     6001 gattccgcaa catcctgtaa ctgcatcaca atagacatta ttttgccaac gccagtttaa
     6061 tgctttccat cacaatgggg tcttccggcg tcatatccgg ggaaaaacgc tggatgacca
     6121 atccgtccct gccaactaaa aatttctcaa aattccataa aatatcatca gggtacagcg
     6181 gtgcacgacc tttgctgacc atacgggcat agaatccgct ctcttccggc gcgactgcgg
     6241 tcggcgctgc ggcaatcaat ttttgataca gcggatggcg tccttcgccg ttaacttcaa
     6301 tcttactgaa catcgggaac gtcacccccc atgtggtggt acagtaagtt ttaatctctt
     6361 catcgctgcc cggttcttgt tccagaaact ggttgcacgg gaatcccagc accacaaaac
     6421 ctcgatcggc ccaggctttc tgaatattct ccaactgctc atattgcggc gttaagccac
     6481 actttgaggc gacattgaca atcaacagca cattaccggc gtacttctcc agcgtggtca
     6541 cttcaccgtc gatatctttc actacggtcg tcagaatgga atcttgcatc gtttctcctg
     6601 ggtgtggtca gtaaaaatct tagcttttaa tcatagaccg tctttttgcg gctaacgtct
     6661 tgcttttaac aataaccaga taaacaccgg cgcacccaac gttgcggtga ccacgccaat
     6721 aagcagctct gcggcagcta atgccaagcg cgctacaata tcggccagca gcaatgcgct
     6781 cgcccctgcc agcgcgcagc cgggaagtaa tacgcgatga tcggttaaac cacacaaccg
     6841 aagaatatgt gggatcacca gaccaataaa gccgatagca cccgccagcg ccacactgac
     6901 gccaaccatc cagccggtcg cggccaccag cacattgcgc cagaaccaca ggggtaaacc
     6961 cagttgccgc gccgagatct cgccaagtgc taacatattc atcggcctgg actgacaaca
     7021 gatccacaac aacacgggga tcaatgccag catcagccag ctttgccgcc agtctacgcc
     7081 gccaaaaccg cccatcatcc agtacatcag ctgacgtaaa tcaacagagg tggaaaagta
     7141 gatagcccac gtcattagtg cgctacagat aatccctaat gcaacgccag ccagcaataa
     7201 ccgactggtc gaaagatgac gacgggcgaa acgtaagagt attaaagtaa tgataagcgc
     7261 gccagcaatc gcacacagcc ctagcgccca gttggggagt tgcccttgcc caagcaatac
     7321 cgcggcgata agccccacgc ctgcgccgtt agagacgcca agtagtccag gttctgccag
     7381 aggattttca aacaacgcct gcattacagc gccggatata gccagcgccg caccaaccaa
     7441 caatacagcc agcgtacgtg gcaggcgaat ttgccagacg aacagttcgc cacgaggagt
     7501 aaaccagtca cctagcgaga tccattgttc accggcgcaa aggcttaaaa gaagcgccag
     7561 cagcatcaaa actgacaggc ataataacca gcgaatattt tgtcgctgtt gttggcgggc
     7621 aagtgtcagc atggtatccg ttctgctgaa gtgtcatggc gttgatttta cggtgactct
     7681 tcgacagtga aaagaaaaaa ggccgcagag cggccataaa cacaagtaaa aatcaacaag
     7741 ttagataatt atcaaagact tacagacaca caaaaacaca gccaaccaca acaaataaca
     7801 gggatgtggt cactttgtgg atcatatcgc cgccataaat ttacttaata acagtaacca
     7861 cagccacaag cacggccatc gcagacagga caatacctgt gagtaaccaa gtctgatttg
     7921 caaatgattt ctgaagttca gtacgatgct cagccatctc aactttcaga gactggcgta
     7981 aatcagccat ctcagacttc agggattggc gcgattctgc catctcaagc ctcaaccctt
     8041 cgcgaatctc cagtacatca gatttggtag caaaggattc gcttcttgtg gtgagagtaa
     8101 tcaggttgtt tttgatttca gtcacatcac tttccaggca actgacccgc cgttcaaggt
     8161 cgtcattcat gccatcacct ccatcattac cacttcctgt tacttggttg ttggatacat
     8221 tataggcatt ctctttcttt tttctgaacc gcgaaatatg tgcaacattc tctgccatca
     8281 cttctcatca ccctgcttgc ctacccagtt cataaaggcc cgggccgagt aacgatgaac
     8341 aaacccacat ctgttacagg taagtcggaa ttcatagtta tggatatttt cccgatcatg
     8401 tccctcatat cccggatacc cagtaaatgg accgatgtaa tcaagcataa caaaagtgcc
     8461 taaatcacac tcctcagcag ttttcggctc aggccccacc tcaacaatat tcgtcaaata
     8521 catgtacata tccgtatcgc cacacgacaa acatttttca ttcgtagacg actcattgag
     8581 aaaacgcgca aaattatcga gcgttgccat tcttttaagt tcatctaaat gaaacttcac
     8641 tcttaagcac ctcctagtgg attaaacctc accgcatcct gcaggtaatc cggcgcaaga
     8701 tgggcataaa tcatcgttgt ctgaatcttt gcgtgcccca gaattttctg gagcg
//
LOCUS       DAJTOR010000170         8653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTOR010000170 DAJTOR010000000
VERSION     DAJTOR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.170"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..271)
                     /locus_tag="OO249_003468"
     CDS             complement(<1..271)
                     /locus_tag="OO249_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5637637.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(365..927)
                     /locus_tag="OO249_003469"
                     /pseudo
     CDS             complement(365..927)
                     /locus_tag="OO249_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1131..1337)
                     /locus_tag="OO249_003470"
     CDS             complement(1131..1337)
                     /locus_tag="OO249_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5637638.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1444..2007)
                     /locus_tag="OO249_003471"
     CDS             complement(1444..2007)
                     /locus_tag="OO249_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5637639.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3382..5217)
                     /locus_tag="OO249_003472"
     CDS             complement(3382..5217)
                     /locus_tag="OO249_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637640.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5318..5605
                     /locus_tag="OO249_003473"
     CDS             5318..5605
                     /locus_tag="OO249_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5637641.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5577..7106
                     /locus_tag="OO249_003474"
     CDS             5577..7106
                     /locus_tag="OO249_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5637642.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7276..8494)
                     /locus_tag="OO249_003475"
                     /pseudo
     CDS             complement(7276..8494)
                     /locus_tag="OO249_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         2682 a         1956 c         1617 g         2398 t
ORIGIN      
        1 tacaccagac atcgttatta agtagcagca ccataagata aggagtggca tcgttagcca
       61 cagcttccct actccagagg taatataaag gggtgggctc gactgattta tctttacgtc
      121 gcttacactg caaatgttca gaaattagtc tatgcagctc accagtaaaa tcagcaatca
      181 gagaaggaat tgccttattc ataccagggc aaggtattaa tttaaactgt aataatttaa
      241 ttttaggatg tgtagcagca gcccgataca gagttgcaag gacacacttt tgccagaggg
      301 cgttacagga aagcttaatg tttgattctg tatacataat aaatcacctt atagttacaa
      361 taggtcaaaa accgctgtag ccaaagttac gctggcctga tgctttagtg tcgagcttcg
      421 tcaaataatc cagacgtacc aataagcgct ggtactgctc tgggaaatta ggatcgttaa
      481 tatccaggat gtaacgtcca ttagcaggga aatgaactaa tccctggtga tcttccagtt
      541 ccaaccctaa ggcactgtac caggctccgg taattaaccc tctcagggtg tcagagcgct
      601 catagtttcc cagatggtaa taggcgtctt tgttaaaaaa aggcagacgt gataatgaca
      661 tttaaaggac tgcgtatatt ctttcgccca gataatacaa accgacgttg cgtaggtgcg
      721 tttgccttgt tgttttttac ggtgttgatc tgcattaatt ttcgcattca gcgagttacg
      781 gaaacgagat atctctccgg ggatcaggtt agggaaacag gtaatgatat ccccgttgtc
      841 aatcacagga gggtaatgga gatccccccg tattgctgtt attctggaag tgtttttaat
      901 attcgtattg atagttctca gagatatatc acactctaga tattttaatt aagtatcagt
      961 tcgatatcgg catattgtac actacctcag gtagatgatg agatcagata cagggatcaa
     1021 ctgacggtga gcgcagtccc cacattttat ccagggctta ctgcatattc ccaacagcca
     1081 ttcgttagcc aggtagacga ataccctgat ttgccgctgg ttttgctttc ctatcgaagg
     1141 gcccatacat catcacgccc acgaaacaac cgacgaaata acgcaacttt ctcatccgtg
     1201 gataatacga aaacgcactg cacagggctc tgcggtttac gtcgccattc aatcccatga
     1261 gcttcaagta atgagaccag ccgggtattt tctgcccgca gtactgctag ttcatcgctg
     1321 tcagatttat aaaccatatg cgttatcact tgtacctgtc tcccggcggc accacgactg
     1381 aaattcgtgt gaaggcaaat ccacgcgata acttacgata gttcggacat aagcccccat
     1441 aattcagtta tgtcgtggtc gaccccggtt acttttttcg ggaagcctgc tacggcccag
     1501 aataataccg tgttctttct gatcctgttt agccagatag ctaagggcgt aacgtaagtc
     1561 gtctaccgca gatttatcac tatagtgaat cacgtgatcg atacgcaccg ggtatttgtc
     1621 tttagcccgg cacagatgaa aataaccctc cccttccgtg atccgcctcc agatatctcc
     1681 cagttgcctg gatatctgat aagagttctt gtgacgttgt ccatccaggt agccgataaa
     1741 atgaatatga agccctttgt ctggtgtata ttccattacc cagtaatatc ccgccaacat
     1801 cgtctgggtt tcgctaagta aacggtatat ttccatacac atactgtgct tacaggaatg
     1861 cccgaaactg ggcgtgtctt tcctgtaagc aaaatcaatt ctgaaaggta acagtttaga
     1921 aaaacgttga aacataccat ccatgtgttc attcacgtct ttcagaatca tgaaatccat
     1981 tttgtaattg ggattggcat tatacatttt atagtcctta ctttataaaa ctcacaggaa
     2041 gagagaatta cgtaaatact gagagtagag aaacgccgta ccacggcatt aataaacgaa
     2101 gtaaaccagc agcacagaca acctgataaa actgttttgt attaccgcat tactgtgatg
     2161 aggtaatact tcagaataca gtgagactct cttaaactga gaaccagtac gaattacaat
     2221 ggaaaaacac acctcataga attgagtttc aggaagcacc actaaattct ttacaggaag
     2281 taatctgaca ttcacaaaac tcagtatcgg ctagctacag gcaacatcgc tattaatatt
     2341 gtcgaaccat taagtaaagc cgagagagac cacgggtatt taatcattag cataaaagaa
     2401 taatggtata cagaacatac ccacacacca taagaagatt gttccataca gaaaagatca
     2461 tatttaccta tacctataaa ctgtaacgga gtcataacac gctctaatag tgttccgata
     2521 cgtaaccagt gttaagtacg tacaggacaa cattacacac ataattcacc atacagcttc
     2581 ccccatatgc ttcactgcgt agtgtatgga gatatatgca ttacacacat acagaatact
     2641 tagcataaag aggagtgaca ttaccacgta aagagataag ttatacaaca aacatacaaa
     2701 caatacggca cagacacaca ataccataac tccaactaat aatactaatt aaccttaatt
     2761 atttatggta tatgaaataa atgtgattct cccggaacta actaacctaa ccagacagat
     2821 gatcactgta taaaaacttc agttaatact catttgtttt gtcgactcag tatataatga
     2881 gtgaccagta atcaggataa tcacacttac ataacctgta aaaaagaaca agaagaataa
     2941 gccactaaca taatgcaaaa tggcatctga atcgatcgca gtttggcatt cgaatcgatc
     3001 atgatttggc ccaatccgtg atcgaataga gcggaatacg caacataatt caccagacaa
     3061 gagcttacac cgaattacag attatgtatt aatgtcaaaa ctggcatatt ttacagatac
     3121 aatgaacatg caacccatca atctaatagg ggcggtatgt acatctgccc atgaatggga
     3181 tggcgctaag aatacaaaac aattagacat agatcacaaa aacacaccat tctaattgaa
     3241 aaacaaaaca aacaaataaa tcatactatt atagaatttt ctcttgccag acatagataa
     3301 tacccctgct aattttgcta atggttagca aaattagcag ggtatataaa gattattgac
     3361 ccaaaacaca ctcagatgaa ttcacgacag ctcaggaggg ttattcctga tgtcatcttc
     3421 acaaataatg gttaacgcat catatatcat tctgcgtctg ttcctgtttt cggccttatc
     3481 gccatacaac atcatcacat ttttaatttc atcgaccggt gatttcacat agtgaactaa
     3541 tacaccaagc ttctcaacac attgcagtaa agtatatttc ttactgcctg tgaaaagccc
     3601 tatcatagtt tctttacgtt cgcgactgga taatactttg ttttccataa tgacttctgc
     3661 cagcgtctta ccatcataaa catactcttt atctatcaga atatttatct tatccagcac
     3721 cttctctacg tcatcaagca aacctgtaat atatgggggc gcaatatatg gtttcgtact
     3781 gatcttttca agcatacaat tatacatctt aataacacgc atcagggtaa aattgttcgc
     3841 atcatactgt atttcaccct ccttatatac aggatcaata agtggtattt tttcaataaa
     3901 tgcatcctga taggctttta catggtcaag ccaataaaac aacgagccat actttatatt
     3961 ttttctattt actttaacct ccagtgctgt ccgaagcaac gataatgccc ccctgacata
     4021 tcctgaaggt aattcaccaa acaatccttt tctttccatg tcatcataat gctgcagcac
     4081 atcattaatt ttatttttcc ttatcagaca aatgtacacc gcaagtgttt cgtagcagct
     4141 ggtcacatga acagcctcat tctgcaactg cccaaaatag cttataatct ttttcagata
     4201 tgcgtcatca caaccattct tatcattaat attaaataca agatctttca gacgggaaat
     4261 atattcctga gatttatcat caagtgaaac actataacga gacaatgttc cttctgcact
     4321 gttttgaaac ttataaaaca tttccagtaa tttttttgca taaggcgttg tatcaaagct
     4381 ggtaaagtca taaagatatt gcgcagcgta accatacaaa ccatgaatcc agagtccttc
     4441 tctcaccaac actctgagat aatatgcaag gctttgaagt atcttcgtct ttcgttgtat
     4501 atcaagtaag acacttaatg aataaaatgc attatagaat ttatcaatat tcctgagcac
     4561 tttctccagt tcatccttat ccagcttctt ctcccggtaa tcctttttat attcattttt
     4621 gatagccatg atatattgtt gcatcaggct cttgcttttc ccgttatcct tccgtatagt
     4681 tcctgtatat ttccccagag catacagctt tccattttca tgaaaatctt ccttgttgaa
     4741 tcccagttca gccagatatt cccgaagata acgtttaaca gcagcttcat cgctttctgt
     4801 atcagcgtga tcaagacaag gacaatacga tttctgtaaa aaattataaa tatgataata
     4861 aaaagcagag tcattctctg gtctgtatgt attatacata tctacaacaa gaaaaataac
     4921 cccaaaagtg cgcagacaat agctgcgaag gaattcaggt tttagtgtgg aatgcactgg
     4981 ataagaattc atcatgtaat aaaaatgttc atatcgggca tatatgatcc gccgttgctc
     5041 ctgactgaac accccttcag catcaataat acgactgatc tcattataac gttgatccca
     5101 gaatttttga tgcatttcaa aattggtact cttccgcttg tgtgccggat tctcgccttc
     5161 cttaaacaac cccaaaacag tacaaatatc atacaacata tttgtaaatg tgcgcatatg
     5221 ctgtgctctc cggttggttt gcattttatc gcagaggaca ttctgagctt aacgtatcaa
     5281 actgacacaa cgacatgcaa tgcatatgga gaacaatatg tcaaatcaaa ccggacagct
     5341 ttacacgcct tcacgcatcc tgcgtatggc tgaacttacc cgactgctgg gtatatcaag
     5401 atctactatt tacgtgaaaa taaatccggc ctcgaaatac cacgacccta cctttcccaa
     5461 accaataagg cttggtagtg catccgtagg ctggcgggca tctgccatcg atgagtggct
     5521 gatgttacat acggcaccag cctggtctga acctgaaaac aaactaaagg aacttaatga
     5581 tgacgacaaa actatcactc cctgaacttg acacaactaa actgcctgac aggtgccagg
     5641 cattacttga tgaaatgcat gaagaaacag gaatacgccg tgaaatactt ctgtctgtta
     5701 tgctgacggt catggctgcc tcggtccagg atacgcatga agttgaactt tccggagggc
     5761 aacgtaccag ccttcagata tttatgtgtc tttcatcagc atcaggcagt ggaaaaacct
     5821 ctgcctgcgc aaaattaatc gctcctgttc atgaaacaga agaagaacta catcaggcat
     5881 atatcgacga taaaaaaaac tatgaccgaa tgatggaaat gtggacaaca gataaaaaaa
     5941 tcctggagcg gagatataaa aaggaaattg aaagatcccc ggaaaatgct gctgctgcac
     6001 gagcagcact tgaaaagtgt atagcaaata aacccgaacc tcccgtacaa caggttctca
     6061 tcgtgaatga cgcgacacca gaagggatag ctttgaaact cagccagtca ccctctcttc
     6121 tgttgctgtc tgatgaagga ggtactattc tggataagcg tttcgaacgc aaatcggcac
     6181 tgtacaacac attatggagt gggcaaccag tcaacgttga acgggcatcc agacccgggt
     6241 tccggctgaa tgatgtccgt ctcactatac ttatactgac tcaaccggta atatttaata
     6301 agttttttac tctcactggc gatcagatcc ggggcaatgg ctttctggcc cggttgctat
     6361 tttgcgaacc cggagcaatt aaaataatga cgacagaacc gcatactgcc actcctgttg
     6421 tgacgcaaca atgtgcaagc tgttttcgta agaaaagttt tggcagtcag atcagagact
     6481 ctctgagagc atccagagaa agacgtgcaa aaagtaaaga gcgcatctgc atgacattat
     6541 caaatgcagc atcccgcgca ctggagcgct ttcacaaaga aaatatggat actgtcagac
     6601 agaatcctca tatgggtaca tttgaagaca ttattgtcag aaaaagagag caggttgtca
     6661 ggatagcagc acttctggaa ctggagaaag atccagactc gacagtaata acgctggaaa
     6721 gtactaatag cgccatttat ctcattgaat tttatttcaa gcatcttata tataaacttg
     6781 aatcccttcg ggaaatatcc ccggctgaga aacttgataa atggcttcag aaaagaatta
     6841 tcacaacagc aggttacata ttccagaaaa gttatatttt acaatatgcc ccatatgctc
     6901 tcaggaaaaa atgtgttctg gatgaagcac ttgacatact ggcggaacaa aggaaaataa
     6961 ggattgatga taacctggtt gtttatatag gaaacacgat tacaccatcc gaactggcaa
     7021 aaaaactaaa tattcctgca ttcgatgccg gagttttcat ttgtgatcat caaaacatac
     7081 tgaagtatca ccgaaacaga ctatagatac tatcggacaa aaaataaatt atcacaagtg
     7141 tattattcgt tataataatg aataatcgtt atctgagtgc aatgccaggt tttcattacg
     7201 aactggcatt gcatttattt ttcaaaaaag aaagcagtaa ccatttggct actctgagta
     7261 tgccagtcca tgaaactaag cgaattcctt agtgttttta gcaagttgag acatcacata
     7321 atccgcccat gcctgcatca tcggtacacc gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cgg
//
LOCUS       DAJTOR010000171         8649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTOR010000171 DAJTOR010000000
VERSION     DAJTOR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.171"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            159..1070
                     /gene="ttdA"
                     /locus_tag="OO249_003476"
     CDS             159..1070
                     /gene="ttdA"
                     /locus_tag="OO249_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR5637643.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1067..1672
                     /gene="ttdB"
                     /locus_tag="OO249_003477"
     CDS             1067..1672
                     /gene="ttdB"
                     /locus_tag="OO249_003477"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5637644.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRGKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1721..3184
                     /gene="ttdT"
                     /locus_tag="OO249_003478"
     CDS             1721..3184
                     /gene="ttdT"
                     /locus_tag="OO249_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5637645.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVFGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(3227..4240)
                     /gene="tsaD"
                     /locus_tag="OO249_003479"
     CDS             complement(3227..4240)
                     /gene="tsaD"
                     /locus_tag="OO249_003479"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5637646.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4477..4692
                     /gene="rpsU"
                     /locus_tag="OO249_003480"
     CDS             4477..4692
                     /gene="rpsU"
                     /locus_tag="OO249_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5637647.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4803..6548
                     /gene="dnaG"
                     /locus_tag="OO249_003481"
     CDS             4803..6548
                     /gene="dnaG"
                     /locus_tag="OO249_003481"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5637648.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6743..8584
                     /gene="rpoD"
                     /locus_tag="OO249_003482"
     CDS             6743..8584
                     /gene="rpoD"
                     /locus_tag="OO249_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5637649.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2086 a         2311 c         2316 g         1936 t
ORIGIN      
        1 tgccattccc tgctctggcg caatattcat tcttattttc gcgttgattc acgaactgtt
       61 aattacatat tcacggtggc aaaaaatata aaaccacatt tttagtggta gtttatggcg
      121 gtgaattttt ccagacaaat acaaaaactg gagttgccat gatgagcgaa agtaataagc
      181 aacaggcagt gaataagttg acagagattg tcgctaactt taccgccatg atttctaccc
      241 gaatgcctga tgacgtggtg gataaactaa aacagctaaa ggatgccgaa acgtcgtcga
      301 tggggaaaat tatctaccat acgatgttcg acaacatgca aaaagcgatt gacctgaatc
      361 gtcctgcctg tcaggacacc ggggagatta tgttcttcgt taaagtcggt tcccgcttcc
      421 cactgcttgg cgagctgcaa agcatactca aacaagccgt ggaagaggca accgtcaaag
      481 cgccactacg tcacaatgcg gtagaaattt ttgacgaagt aaacaccggc aaaaataccg
      541 gtagcggcgt accgtgggtc acctgggaca tcatccccga caatgacgat gcggaaatcg
      601 aagtttacat ggcaggtggc ggctgcacgc tacctggccg ctcgaaagtg ttaatgccgt
      661 cagaaggcta cgaaggcgtg gtgaaattcg tcttcgaaaa tatctccacc ctcgccgtaa
      721 acgcctgtcc accggtactg gtgggcgtgg gcatcgccac ctcggtggaa accgccgccg
      781 tactctcgcg taaagccatt ttgcgcccga ttggcagccg ccatcccaat ccaaaagcgg
      841 cagaactgga gctacgcctg gaagaaggac tcaaccgtct ggggattggt ccacaagggc
      901 tgactggcaa cagttcagtg atgggcgtac atatcgaatc tgccgcccgc catccgtcaa
      961 ccatcggcgt tgctgtctct accggctgct gggcgcatcg tcgcggcaca ctgctggttc
     1021 atgccgatct cacctttgaa aatctgtctc acacccggag cgcgttatga aaaagatcct
     1081 gacaaccccg atcaaagctg aagatctgca agatattcgc gtcggcgatg tgatctacct
     1141 gaccggtacg ctggtgacct gccgtgacgt ttgtcaccgc cgcttgatcg aactgaaacg
     1201 tccgatccct tacgatctca acggcaaagc gattttccac gctggcccca tcgtgcgcaa
     1261 aaacggcgat aaatgggaga tggtttccgt tggcccgaca accagtatgc gtatggaaag
     1321 ttttgaacgt gaatttatcg agcagaccgg cgtgaaactg gtggttggca aaggcggaat
     1381 ggggccgctg accgaagaag gctgccagaa attcaaggcg ctacatgtga ttttcccggc
     1441 aggctgcgcg gtgctggcgg caacccaggt ggaagagatt gaagaagtgc actggacaga
     1501 gctcggaatg ccggagtcgc tgtgggtctg ccggggcaaa gagttcggcc cgctgattgt
     1561 ctctattgat acccacggca acaatctgat agccgaaaac aaaaagctgt tcgccgaacg
     1621 ccgcgatccc atcgtggaag agatctgcga gcacgtccat tacatcaaat aaccctcccg
     1681 gagaggctcg ccccctctcc tttttcgcag gcataacacg atgaaacctt ccactgaatg
     1741 gtggcgatac cttgcgccgc tggcggtcat cgccattatt gctctaattc cggttcccgc
     1801 agggctggag agtcatacct ggctctactt tgccgttttt actggcgtga tcgttggact
     1861 gatcctcgaa cccgtgccgg gtgccgtggt ggcgatggtg ggtatctcca tcatcgccat
     1921 actctctccc tggctgctgt tcagccccga acagctcgcc cagccaggct ttaagttcac
     1981 cgccaaatcc ctctcctggg cagtttttgg tttttctaat tcggttatct ggctcatttt
     2041 cgccgccttt atgtttggca caggctatga aaaaaccggg cttggacgcc gcattgcgct
     2101 gattctggtg aaaaagatgg gacatcgcac gctgtttctc ggctatgcgg tgatgttctc
     2161 cgagctgatc ctcgcacctg taacaccgtc caactcggcg cgtggtgcgg ggattatcta
     2221 tcccatcatc cgtaacctgc caccgctcta tcaatcacaa ccaaacgaca gcagttcgcg
     2281 cagcattggc tcgtacatca tgtggatggg gattgttgcc gactgcgtga ccagcgccat
     2341 tttcttgacg gcgatggcac caaacttgct gttaattgga ctgatgaaaa gcgcatctca
     2401 cgccacgctg agttggggcg actggttcct cgggatgttg ccgctcagta ttttgctggt
     2461 gctgctggtt ccctggctgg cttatgtgct gtacccgccg gtactgaagt ctggcgatca
     2521 ggtgccgcgc tgggcagaga cggaactgca ggcaatgggc ccgctctgtt cgcgtgaaaa
     2581 acggatgctg gggctgatgg taggcgcgct ggtgctgtgg attttcggcg gtgattatat
     2641 cgatgccgcg atggtcggtt acagcgtggt ggcactgatg ctgcttctgc gcattatcag
     2701 ttgggacgac attgtcagta ataaagcggc gtggaacgtt ttcttctggc tggcctcgct
     2761 tatcaccctc gctaccggac tcaacaacac tggttttatt agctggtttg gcaaactgtt
     2821 agcaggcagc ttaagcggtt attcgccaac gatggtgatg gtggcgttga ttgtggtgtt
     2881 ttatctgctg cgctactttt tcgccagcgc cacggcgtat acctccgccc tcgcaccgat
     2941 gatgattgcc gctgcgctgg cgatgccgga aatcccgctg ccggtgttct gcctaatggt
     3001 tggcgcggca attggtctgg ggagcattct tacaccatac gccaccggac ccagtccgat
     3061 ttactacggt agtggttatc tgccaacggc ggattactgg cgactggggg cgatttttgg
     3121 gctgatattc ctcgtgttgc tggtgattac cggcttactg tggatgcccg tggtgttgct
     3181 ttaagtgagg acggtgccgg aaattgctct ccggcaccca atagttttac gcagccggta
     3241 actccgccag cggccagcgc ggacgcacgc taacgccgag atccgccgtc gcgcctgctt
     3301 taaaccgcac cattccggca taggcgatca tcgcgccgtt atcagtacaa aactccggac
     3361 gcgcgtagaa cacttcgccg cggcgttttt tcatcatttc cgccagcttc gcccgtaacg
     3421 tgcggttagc actcacgccg cccgccatga ccagtcgctt aaagcccgtc tgatccaacg
     3481 ctcgcttaca cttaatcatc aacgtatcga ccaccgcatc ttcaaaggcg cgggcgatat
     3541 cagcacgcgt ctggtcgtcg gtgccgttgt cacgaatcgt gttcgccgca aaggttttca
     3601 gaccagaaaa gctgaaatcc agccccggac ggtcggtcat cggacgcggg aaaacaaagc
     3661 gcccggcagt accctgagcc gccattttcg acagtaacgg tccgccagga taatccagcc
     3721 ccagcagctt cgcggtttta tcaaacgctt caccggcggc atcatcgata gactcgccga
     3781 gcagctcgta ctgaccaatg ccagtcacgc tgattaactg cgtatgaccg ccggacacca
     3841 gcagcgcgac aaacggaaat tccggcgggt tatcttccag catcggcgct aacagatgcc
     3901 cttccatatg gtgtaccggg attgccggaa cgttccaggc aaacgccaga gaacgcccca
     3961 cggttgcgcc aaccagcagt gcgccgacta atccagggcc tgcggtatag gccacagcat
     4021 caatgtcttt tgccgttaag ccagactcct ttagcgccgc ctggatcaac ggtacggttt
     4081 tgcgcacgtg atcgcgggag gccagttcag gcacgacgcc gccgtagtca gcgtgcaatt
     4141 tcacctgact atacaattgg ttggctaaca aacctttttc atcgtcgtaa atggcgatgc
     4201 cggtttcatc gcaggaagtt tcaataccca gtacacgcat gacttgtttt acctcgcttt
     4261 attaccgcgc agtgtaggac caatgcgggt tgatgtaaaa ctttgttcgc ccctggagaa
     4321 agcctcgtgt atactcctca cccttataaa agtccctttc aaaaaggccg cggtgcttta
     4381 caaagcagca gcaattgcag taaaattccg caccattttg aaataagctg gcgttgatgc
     4441 cagcggcaaa ccgaattaat caaaggtgag aggcacatgc cggtaattaa agtacgtgaa
     4501 aacgagccgt tcgacgtagc tctgcgtcgc ttcaagcgtt cctgcgaaaa agcaggtgtt
     4561 ctggcggaag ttcgtcgtcg tgagttctat gaaaaaccga ctaccgaacg taagcgcgct
     4621 aaagcttctg cagtgaaacg tcacgcgaag aaactggctc gcgaaaacgc acgccgcact
     4681 cgtctgtact aattccccga gagcgttgct ctccgatcag accgagttgt agttgtaagg
     4741 ccgtgcttcc gaaaggaatg cgcggcttat tttcgtttat gaattgctaa aaatcggggc
     4801 ctatggctgg acgaatccca cgcgtattca ttaatgatct actggcacgc actgacatcg
     4861 tcgatctgat cgatgcccgt gtgaagctga aaaagcaggg caagaatttc cacgcgtgtt
     4921 gtccattcca caacgagaaa accccgtcat tcaccgttaa cggtgagaaa cagttttacc
     4981 actgctttgg atgtggcgcg cacggcaacg cgatcgactt cctgatgaac tacgacaagc
     5041 ttgagttcgt cgaaacggtc gaagagctgg cagcaatgca caatcttgaa gtgccatttg
     5101 aagcaggcag cggccccagc cagatcgagc gccatcagag gcaaacgctt tatcagttga
     5161 tggacggtct gaatacgttt taccaacaat ctttacaaca acctgttgcc acgtctgcgc
     5221 gccagtatct ggaaaaacgc ggattaagcc acgaggttat cgctcgcttt gcgattggtt
     5281 ttgcgccccc cggctgggac aacgtcctga agcggtttgg cggcaatcca gaaaatcgcc
     5341 agtcattgat tgatgcgggg atgttggtca ctaacgatca gggacgcagt tacgatcgtt
     5401 tccgcgagcg ggtgatgttc cccattcgcg ataaacgcgg tcgggtgatt ggttttggcg
     5461 ggcgcgtgct gggcaacgat acccccaaat acctgaactc gccggaaaca gacattttcc
     5521 ataaaggccg ccagctttac ggtctttatg aagcgcagca ggataacgct gaacccaatc
     5581 gtctgcttgt ggtcgaaggc tatatggacg tggtggcgct ggcgcaatac ggcattaatt
     5641 acgccgttgc gtcgttaggt acgtcaacca ccgccgatca catacaactg ttgttccgcg
     5701 cgaccaacaa tgtcatttgc tgttatgacg gcgaccgtgc aggccgtgat gccgcatggc
     5761 gagcgctgga aacggcgctg ccttacatga cagacggccg tcagctacgc tttatgtttt
     5821 tgccggatgg cgaagaccct gacacgctgg tacgaaaaga aggtaaagaa gcgtttgaag
     5881 cgcggatgga gcaggcgatg ccactctccg catttctgtt taacagcctg atgccgcaag
     5941 ttgatctgag tacccctgac gggcgcgcac gtttgagtac gctggcactg ccattgatat
     6001 cgcaagtgcc gggcgaaacg ctgcgaatat atcttcgtca ggaattaggc aacaaattag
     6061 gcatacttga tgacagccag cttgaacgat taatgccaaa agcggcagag agcggcgttt
     6121 ctcgccctgt tccgcagcta aaacgcacga ccatgcgtat acttataggg ttgctggtgc
     6181 aaaatccaga attagcgacg ttggtcccgc cgcttgagaa tctggatgaa aataagctcc
     6241 ctggacttgg cttattcaga gaactggtca acacttgtct ctcccagcca ggtctgacca
     6301 ccgggcaact tttagagcac tatcgtggta caaataatgc tgccaccctt gaaaaactgt
     6361 cgatgtggga cgatatagca gataagaata ttgctgagca aaccttcacc gactcactca
     6421 accatatgtt tgattcgctg cttgaactgc gccaggaaga gttaatcgct cgtgagcgca
     6481 cgcatggttt aagcaacgaa gaacgcctgg agctctggac attaaaccag gagctggcga
     6541 aaaagtgatt taacggctta agtgccgaag agcgatcggg aagcccccga cagccgcact
     6601 gagaggcagc ggcaaatata taagtacgcc ctcgtaatta tcgttggcgg taaacaaccg
     6661 ttggatttca gcgttaacgg ctgaaggaca tcgggtcaat cgcccaacac caacctcatg
     6721 aaataagtgt ggataccgtc ttatggagca aaacccgcag tcacagctga aacttcttgt
     6781 cacccgtggt aaggagcaag gctatctgac ctatgccgag gtcaatgacc atctgccgga
     6841 agatatcgtc gattcagatc agatcgaaga catcatccaa atgatcaacg acatgggcat
     6901 tcaggtgatg gaagaagcac cggatgccga tgatctgatg ctggctgaaa acaccgcgga
     6961 cgaagatgct gccgaagccg ccgcgcaggt gctttccagc gtggaatctg aaatcgggcg
     7021 cacgactgac ccggtacgca tgtacatgcg tgaaatgggc accgttgaac tgttgacccg
     7081 cgaaggcgaa attgacatcg ctaagcgtat tgaagacggg atcaaccagg ttcaatgctc
     7141 cgttgctgaa tatccggaag cgatcaccta tctgctggaa cagtacgatc gtgttgaagc
     7201 agaagaagcg cgtctgtccg atctgatcac cggctttgtt gacccgaacg cagaagaaga
     7261 tctggcacct accgccactc acgtcggttc tgagctttcc caggaagatc tggacgatga
     7321 cgaagatgaa gacgaagaag atggcgatga cgacagcgcc gatgatgaca acagcatcga
     7381 cccggaactg gctcgcgaaa aatttgcgga actacgcgct cagtacgttg taacgcgtga
     7441 caccatcaaa gcgaaaggtc gcagtcacgc tgccgctcag gaagagatcc tgaaactgtc
     7501 tgaagtattc aaacagttcc gcctggtgcc gaagcagttt gactacctgg tcaacagcat
     7561 gcgcgtcatg atggaccgcg ttcgtacgca agaacgtctg atcatgaagc tctgcgttga
     7621 gcagtgcaaa atgccgaaga aaaacttcat taccctgttt accggcaacg aaaccagcga
     7681 tacctggttc aacgcggcaa ttgcgatgaa caagccgtgg tcggaaaaac tgcacgatgt
     7741 ctctgaagaa gtgcatcgcg ccctgcagaa actgcagcag attgaagaag aaaccggcct
     7801 gaccatcgag caggttaaag atatcaaccg tcgtatgtcc atcggtgaag cgaaagcccg
     7861 ccgtgcgaag aaagagatgg ttgaagcgaa cttacgtctg gttatttcta tcgccaagaa
     7921 atacaccaac cgtggcttgc agttccttga cctgattcag gaaggcaaca tcggtctgat
     7981 gaaagcggtt gataaattcg aataccgccg tggttacaag ttctccacct acgcaacctg
     8041 gtggatccgt caggcgatca cccgttctat cgcggatcag gcgcgcacca tccgtattcc
     8101 ggtgcatatg attgagacta tcaacaaact caaccgtatt tctcgccaga tgctgcaaga
     8161 gatgggccgt gaaccgacgc cggaagaact ggctgaacgt atgctgatgc cggaagacaa
     8221 gatccgcaaa gtgctgaaga tcgccaaaga gccaatctcc atggaaacgc cgatcggtga
     8281 tgatgaagat tcgcatctgg gggatttcat cgaggatacc accctcgagc tgccgctgga
     8341 ttctgcgacc accgaaagcc tgcgtgcggc aacgcacgac gtgctggctg gcctgaccgc
     8401 gcgtgaagcg aaagttctgc gtatgcgttt cggtatcgat atgaacaccg accacacgct
     8461 ggaagaagtg ggtaaacagt tcgacgttac ccgcgaacgt atccgtcaga tcgaagcgaa
     8521 ggcgctgcgc aaactgcgtc acccgagccg ttctgaagtg ttgcgtagct tcctggacga
     8581 ttaatcggta ggccggatca ggcgttacgc cgcacccggc actagtccct ctgcacaaac
     8641 gccaccttt
//
LOCUS       DAJTOR010000172         8623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTOR010000172 DAJTOR010000000
VERSION     DAJTOR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.172"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..190
                     /locus_tag="OO249_003483"
     CDS             <1..190
                     /locus_tag="OO249_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637650.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="OO249_003484"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="OO249_003484"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5637651.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="OO249_003485"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="OO249_003485"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5637652.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO249_003486"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO249_003486"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5637653.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /gene="dgkA"
                     /locus_tag="OO249_003487"
     CDS             4544..4912
                     /gene="dgkA"
                     /locus_tag="OO249_003487"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5637654.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="OO249_003488"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="OO249_003488"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5637655.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5703..7028
                     /gene="dinF"
                     /locus_tag="OO249_003489"
     CDS             5703..7028
                     /gene="dinF"
                     /locus_tag="OO249_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5637656.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7144..7353
                     /locus_tag="OO249_003490"
     CDS             7144..7353
                     /locus_tag="OO249_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5637657.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO249_003491"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO249_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5637658.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8228..8623
                     /locus_tag="OO249_003492"
                     /pseudo
     CDS             8228..8623
                     /locus_tag="OO249_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
BASE COUNT         2033 a         2047 c         2310 g         2233 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattct acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacgg cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcact ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catggctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gtaatgttga ttttctgttt tgtaggtaag gtgttatgtt gccttgtcgt
     8041 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac
     8101 tcgctattta tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt
     8161 ttgatagtga ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag
     8221 atgaatcatg ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc
     8281 aaatcaattc gataatgaag atgcaatcaa agataaatta gataatagag gttcaatcaa
     8341 aggtaaatta aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga
     8401 tataattatt gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc
     8461 acgtaaaaaa atgtgttttt aattgagcaa ataaatgatc tcaaaatgtg ggtcaataag
     8521 tatattgacg attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt
     8581 gaccgggcta tttttcattt cgcgattaat agtatatgta atc
//
LOCUS       DAJTOR010000173         8491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTOR010000173 DAJTOR010000000
VERSION     DAJTOR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.173"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..66
                     /locus_tag="OO249_003493"
     CDS             <1..66
                     /locus_tag="OO249_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5637659.1"
                     /translation="RYQAKYVSAIWQKLIPSQADF"
     gene            125..328
                     /locus_tag="OO249_003494"
     CDS             125..328
                     /locus_tag="OO249_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCR5637660.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENELPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            328..1353
                     /locus_tag="OO249_003495"
                     /pseudo
     CDS             328..1353
                     /locus_tag="OO249_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1381..1470)
                     /locus_tag="OO249_003496"
     tRNA            complement(1381..1470)
                     /locus_tag="OO249_003496"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1434..1436),aa:Ser,seq:cga)
     gene            1564..2361
                     /gene="mtfA"
                     /locus_tag="OO249_003497"
     CDS             1564..2361
                     /gene="mtfA"
                     /locus_tag="OO249_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5637661.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2462..2537
                     /locus_tag="OO249_003498"
     tRNA            2462..2537
                     /locus_tag="OO249_003498"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2495..2497,aa:Asn,seq:gtt)
     gene            2851..>8491
                     /locus_tag="OO249_003499"
     CDS             2851..>8491
                     /locus_tag="OO249_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5637662.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYILGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFNNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAESWLPSWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     VGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVMVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGVAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLKALNTNENGIAEVTLKGTVLGVYTAEAILLNGKSDTKIVNIVPDTSNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGMHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTKPADTQLSQSTSNTNDSGVAEVTLKGTVLGVHT
                     VEATLPNGNGYTTTVNIAPDTSNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNGVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEKTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGETTSLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQLTATLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTAS
                     MTGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGM"
BASE COUNT         2433 a         2031 c         2065 g         1962 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagctatc tggcaaaaac tgatcccgag tcaggctgat
       61 ttttaatgtt caaccctaat tgccgctaac cgtatatagt tagcggcggt tatgagatat
      121 agctatgagc agcttatttt taaccgaaga tgaattgcta atattaacgg gctgcaaata
      181 tgcaagccac cagcgaaaat ggttaatgga aaacgagctt ccgttctata ccaatcgtag
      241 tggcaaaccg attgtcagcc gggatctatt tacctgcaat aaaactttac caccacgcga
      301 ggtagagccg aattttggtg cgatctgatg ggaagacgaa ggaaaaatcc tgagcacgaa
      361 aaattacctc caaatgtata cccaaataaa tatagttatg tatggaaacc aacatccagc
      421 gaatctgtaa cactaaccgc catcaaggat ggtttagctg ctttatggaa aaagtatgag
      481 gaaactgtaa ataatcgcga tcgtgcaatg acattcggtc gcttgtggga aaaattcctc
      541 gccagcgcct attacagtga cctcagtcca agaacacaaa aagattatct gcaacatcaa
      601 aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca gaacacatcc
      661 gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat gaagtcccgc
      721 gtttacagtt gggggtatga gcgagggtac gtgaaggcta acccatgtgc aggtgtaagt
      781 aaattcaagg ccaaaaaccg cgaacgatat gtaaccgaca aagaatacca ggcagtatta
      841 agcgttgcac ctcttcctgt ttttatcgca atggaaattg cctatctgtg tgcagcgagg
      901 gtttccgatg tgttatcgct gaaatgggag cagattggaa acgacgggat ctttatccag
      961 caagggaaaa caggaaaaaa acagataaaa gcatggagtc cacgattaca ggcggcgatc
     1021 gaaaaagcaa aacagttacc aacatccgcc tatgtaatca gcaatcaata cggcaaccga
     1081 tatatgtaca aaggctttaa cgaaatgtgg gtagaagcaa gaaatcgcgc aggcaaaatt
     1141 tcaggtattt taaccgactt cacctttcat gatctgaagg cgaaaggaat ttcagactat
     1201 gaaggaagca gtcgggataa gcaacttttc tctggtcaca aaaccgaggg gcaagtgcta
     1261 atctatgaca ggaaggttaa agtttcaccg acacttgatg tcccgttacc tgaaaatatt
     1321 ccaagtaatt ccacgtgtga tttttgtcac tgacttaatg atgtataagt gattgaattt
     1381 tggcggagag agggggattt gaacccccgg tggagttgcc cccactccgg ttttcgagac
     1441 cggtccgttc agccgctccg gcatctctcc gttcagatgg ttgccatgat gccaggaaat
     1501 ttggcatttt aacagtccct gtccgtgcaa ttttgttcaa gtgacgagtt tgcgagcaaa
     1561 acgatgatta agtggccctg gaaagtacaa gaatcagcac atcaaactgc ccttccctgg
     1621 caggaagcac tatcgatccc ccttttaacg tgtctgacgg aacaggaaca aagcaaatta
     1681 gtcactcttg ccgaacgttt tttacagcaa aagcggcttg ttcctttaca gggctttgaa
     1741 ctggattcat taagaagctg ccggatagca cttctatttt gcctacccgt tctggagtta
     1801 ggactggaat ggctggatgg ttttcatgaa gtcttaattt atcctgcgcc atttgtggtc
     1861 gatgatgaat gggaagacga tatcggtctg gtgcataacc aacgtattgt tcagtcaggt
     1921 cagagctggc agcaagggcc tatcgttttg aactggttgg atatacaaga ttcttttgat
     1981 gcttctggtt ttaacctgat tattcatgaa gttgctcata agctggacac ccgtaacggc
     2041 gatcgcgcca gcggagttcc ctttattccg ttgcgtgagg ttgctggctg ggaacacgat
     2101 cttcatgctg caatgaacaa cattcaggaa gaaatcgaat tggttggcga gaatgcggcg
     2161 agcattgatg cttatgcagc cagcgatcct gctgaatgtt ttgccgtact ttctgaatat
     2221 ttctttagcg ccccagaact ttttgctcct cgtttccctt cattgtggca acgtttctgc
     2281 caattttatc aacaagatcc tttgcagaga ctgcatcacg ctaatgatac agactcgttt
     2341 tcggcgacga atgttcatta attaacaact ttgcatatta attaatcaat tgaaatgact
     2401 tatgaaattt agtgttgaca gacaaggtac cgctaagtaa tatgcgcccc gttcacacga
     2461 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     2521 tccagtcaga ggagccaaat tctaaaaatt cgctttttta gcgcaatgtc actgacctta
     2581 gttgaacatt gttttttaac ggatagcggg tttttaacat cttaagcgcc ctcgaccttt
     2641 atggttgagg gcgttttgct atgaacgcca tcaccatttt cccctcgatt ataaaacttg
     2701 agttattcag tagtctcccc tcttgcaact cacacccaaa actgcctaac gaaaagttat
     2761 taattttcaa tcatattgct atcagtattt acattttttc gctgtgctag aaagggcgca
     2821 tttatgttag ctcgttcagg gaaggtaagc atggctacga agaagagaag tggagaagaa
     2881 ataaatgacc gacaaatatt atgcgggatg ggaattaaac tacgccgctt aactgcgggt
     2941 atctgtctga taactcaact tgcgttccct atggctgcgg cagcacaagg tgtggtaaac
     3001 gccgcaaccc aacaaccagt tcctgcacaa attgccattg caaatgccaa tacggtgccc
     3061 tacatccttg gagcgctgga atcggcccaa agcgttgccg aacgtttcgg tatttcggtg
     3121 gctgagttac gcaaactcaa ccagtttcgt acgtttgctc gaggttttaa taatgtccgc
     3181 cagggtgatg aactggatgt cccggcacaa gttagtgaaa aaaaattaac cccgccgccg
     3241 ggtaatagca gtgacaacct cgagcaacag atagccagta cttcacagca aatcgggtct
     3301 ctgctcgccg aagatatgaa cagcgagcaa gcggcaaata tggcgcgtgg atgggcctct
     3361 tctcaggctt caggcgcaat gacagactgg ttaagccgct tcggtaccgc aagaatcacg
     3421 ctgggcgtgg atgaagattt tagcctgaag aactcccagt tcgattttct ccatccgtgg
     3481 tatgaaacgc ctgataatct ctttttcagt cagcatactc tccatcgtac tgacgagcgt
     3541 acgcagatta acaacggctt aggttggcgt catttcactc ccacatggat gtcaggcatc
     3601 aacttctttt tcgaccacga tcttagccgt taccactccc gcgccggcat tggcgcggag
     3661 tactggcgcg actatctaaa attaagcagt aacggctatt tgcgactgac caactggcgc
     3721 agcgcacctg aactggacaa cgattatgaa gcacgcccgg ccaatggctg ggatgtacgc
     3781 gcagaaagct ggctaccctc ctggccgcac cttggcggta aactggtcta tgaacagtat
     3841 tatggcgatg aagtggccct gttcgataaa gacgatcggc aaagtaatcc tcatgccata
     3901 accgctggac ttaactatac ccccttcccg ctgatgacct tcagcgcgga gcaacgccag
     3961 ggtaaacagg gcgaaaatga cacccgtttt gccgtcgatt ttacctggca acctggcagc
     4021 gcaatgcaga aacagcttga cccgaatgaa gtcgctgcac ggcgtagcct tgcaggcagc
     4081 cgttatgatc tggtggatcg caacaacaat atcgttctgg aatatcgcaa aaaagaactg
     4141 gttcgcctga ccctgacaga ccccgtgaca gggaagtcag gagaagtgaa atcactggtt
     4201 tcgtcgctac aaaccaaata tgccctgaaa ggctataacg tcgaagccac cgcactggaa
     4261 gctgccggtg gcaaagtggt cacaacgggt aaagatattc tggttaccct gccggcttac
     4321 cggttcacca gtacgccaga aaccgataac acctggccga ttgaagtcac cgccgaagat
     4381 gtcaaaggca atttgtcgaa tcgtgaacag agcatggtgg tcgttcaggc acctacgcta
     4441 agccagaaag attcctcggt atcgttaagt acccaaacat tgaacgcgga ttcccattca
     4501 accgccacac tgacttttat tgcgcatgat gcagcaggta atcctgttgt cgggctggtg
     4561 ctctcgacgc gtcacgaagg tgttcaggac atcacccttt ctgactggaa agataatggt
     4621 gacggaagct atacccagat cctgaccaca ggagcgatgt ctggcacgct gacgctgatg
     4681 ccacagctga atggtgtgga tgcggctaaa gcccccgccg tggtgaatat catttctgtt
     4741 tcgtcatccc gaactcactc gtcaattaag attgataagg accgttatct ctccggcaat
     4801 cctatcgagg tgacggtaga actgagagat gaaaatgaca aacctgttaa ggaacaaaaa
     4861 cagcaactga ataacgcagt cagcatcgac aacgtgaaac caggagtcac tacagactgg
     4921 aaagaaaccg cagatggcgt ctataaggcg acctataccg cctataccaa aggcagtgga
     4981 cttactgcga agctattaat gcaaaactgg aatgaagatt tgcataccgc tggatttatc
     5041 atcgacgcca acccgcagtc agcgaaaatt gcgacattat ctgccagcaa taatggtgtg
     5101 ctcgccaatg agaatgcagc aaacaccgtc tcggtcaatg tcgctgatga aggaagcaac
     5161 ccaatcaatg atcataccgt cacgtttgcg gtattaagcg gatcggcaac ttccttcaac
     5221 aatcaaaaca ccgcaaaaac ggatgttaat ggtctggcga cttttgatct gaaaagtagt
     5281 aagcaggaag acaacacggt tgaagtcacc cttgaaaatg gcgtgaaaca aacgttaatc
     5341 gtcagttttg tcggcgactc gagtaccgcg caggttgatc tgcagaagtc gaaaaatgaa
     5401 gtggttgctg acggcaatga cagcgccaca atgaccgcga ccgtccggga tgcaaaaggc
     5461 aacctgctca atgacgtcat ggtcactttc aatgttaatt cagcagaggc gaaactgagc
     5521 caaaccgaag tgaatagcca cgacgggatc gccacagcta cgctgaccag tttgaaaaat
     5581 ggtgattata gggttacggc ctctgtgagc tctggttccc aggctaatca acaggtgaat
     5641 tttatcggtg atcaaagtac tgctgccctg accctcagtg tgccttcagg tgatatcacc
     5701 gtcaccaaca cagctccgca acatatgact gcaaccttgc aggataaaaa tggcaaccca
     5761 ctaaaagata aagaaatcac cttctctgta ccaaacgacg tcgcaagtcg gttctcgatt
     5821 agcaacagcg gaaaaggcat gacggatagc aacggggttg caatcgcctc cctgaccggc
     5881 acgttagcgg gcacgcatat gatcacggct cgtctggcta acagcaatgt cagcgataca
     5941 cagccaatga cgtttgtggc ggataaagac agagcggttg tcgttctgca aacatcgaaa
     6001 gcggaaatca ttgggaatgg cgtggatgag acaactctga cagcaacagt gaaagatcct
     6061 tttgataacg tggttaaaaa tctgtcagta gtcttccgca cctcccccgc agacacgcaa
     6121 ctgagtctga aggcgcttaa tactaatgag aacggtattg ctgaagttac ccttaagggc
     6181 acggttttgg gtgtttatac agccgaagcc atactgctta acggtaaaag cgatacgaaa
     6241 atcgtcaata ttgtgcccga taccagcaac gcgcaggtca ccctgaacat cccagctcaa
     6301 caggtggtga cgaataacag cgacagcgtg cagctgacgg cgacggtgaa agatccgtcg
     6361 aatcatccgg tggcgggaat aacggtgaac ttcaccatgc ctcaggacgt ggcggcaaac
     6421 tttacccttg aaaataacgg tattgccatc actcaggcca atggcgaagc gcatgtcacc
     6481 ctcaaaggta aaaaagcggg catgcatacg gttaccgcaa cgttgggtaa caataatgcc
     6541 agcgatgcgc aacctgtcac attcgtggcg gataaggaca gcgcagttgt cgttctgcaa
     6601 acatcgaaag cggaaatcat tgggaatggc gtggatgaga cgactctaac agcaacagtg
     6661 aaagatcctt ttgataacgc agtaaaagat ctacaggtta ccttcagtac caagccggca
     6721 gatactcaac ttagtcagag cacaagcaat actaacgaca gtggtgttgc tgaagttacc
     6781 cttaagggca cggttttggg cgttcataca gtcgaagcca cacttcctaa cggcaacggc
     6841 tatacaacga cagtcaatat tgcgcccgat accagcaacg cgcaggttac gctgaacatc
     6901 cctgctcaac aggtggtgac gaataacagt gacagcgtgc agcttacggc gatggtgaaa
     6961 gatccgtcga atcatccggt ggcggggata acggtcaact tcaccatgcc acaggacgtt
     7021 gcggcaaact ttaccctcga aaataacggc attgccatca cccaagccaa tggggaagcg
     7081 catgtcacgc tgaaaggtaa aaaagcgggt acgcatacgg ttaccgcaac gctgggtaat
     7141 aacaatacca gtgattcgca gccggtaacg tttgtggcgg acaaaacctc ggctcaggtt
     7201 gtcctgcaga tgtcaaaaga tgagatcaca ggtaatggcg tcgataacgc aacgctaact
     7261 gcaacggtta aagatcagtt cgacaatggg gtgaataatc ttccggtaac attcagctca
     7321 gcctcttcag gactcaccct gaccccggga gtaagtaata ccaatgagtc tggcatcgcg
     7381 caggccactc tcgcaggcgt tgcctttggt gagaagacgg ttactgcatc actggctaat
     7441 aatggtgcca gcgacaacaa aactgtgcat tttattggcg acacagcggc ggcaaaaatt
     7501 atcgagttga cgcctgtccc agacagcata atcgccggta ccccgcagaa cagctccggc
     7561 agcgtcatca ccgccacagt cgttgataat aatggctttc cggtgaaagg tgtgactgtg
     7621 aacttcacca gcaacgcagc gacagccgaa atgacgaacg gcggtcaagc cgtgacgaac
     7681 gaacagggta aggctaccgt cacttatacc aatacccgct cctcgataga atcaggagcg
     7741 agaccggata ccgttgaggc cagtctggaa aatggtagct ccacgcttag cacatcaatt
     7801 aatgtcaacg ctgatgcgtc tacggcacat ctcaccttgc tacaggcact ttttgataca
     7861 gtctccgcag gcgagacaac cagtctgtat attgaggtga aggataatta cggcaacggt
     7921 gtcccccagc aggaggtaac cctcagcgtt tcaccaagtg aaggcgtgac ccccagtaat
     7981 aacgctatat atactaccaa ccacgacggc aatttttacg caagctttac cgctacaaaa
     8041 gccggggttt atcaattgac ggcaaccctc gaaaatggcg attcgatgca acaaacagtg
     8101 acctatgtgc cgaacgtcgc gaatgctgaa atcacgctgg cagcctcgaa ggatccggtg
     8161 attgccgaca ataacgatct cacgacacta acagcaacag tcgctgatac agagggcaat
     8221 gcgatagcca acactgaggt aacatttact ctgccggaag atgtgaaggc gaacttcacg
     8281 ctgagcgatg gcggtaaagc gattactgat gctgaaggca aagcgaaagt cacgctgaaa
     8341 ggtacaaaag caggcgctca tactgttaca gcatcgatga ctggcggtaa gagtgagcag
     8401 ttggtggtga actttattgc ggatacgctc actgcgcagg ttaatcttaa cgttaccgag
     8461 gacaatttta tcgctaataa cgtcgggatg a
//
LOCUS       DAJTOR010000174         8460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTOR010000174 DAJTOR010000000
VERSION     DAJTOR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.174"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            98..274
                     /locus_tag="OO249_003500"
     CDS             98..274
                     /locus_tag="OO249_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5637663.1"
                     /translation="MKRLNKLRGWRKVSRSAILREAVEQYLERQQFPVRKAKGGRQKG
                     EVVGVDDQCKEHKE"
     gene            493..735
                     /locus_tag="OO249_003501"
     CDS             493..735
                     /locus_tag="OO249_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637664.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1065..1994)
                     /gene="fdhE"
                     /locus_tag="OO249_003502"
     CDS             complement(1065..1994)
                     /gene="fdhE"
                     /locus_tag="OO249_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5637665.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1991..2626)
                     /gene="fdoI"
                     /locus_tag="OO249_003503"
     CDS             complement(1991..2626)
                     /gene="fdoI"
                     /locus_tag="OO249_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5637666.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2623..3525)
                     /gene="fdoH"
                     /locus_tag="OO249_003504"
     CDS             complement(2623..3525)
                     /gene="fdoH"
                     /locus_tag="OO249_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5637667.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3538..6588)
                     /gene="fdnG"
                     /locus_tag="OO249_003505"
     CDS             complement(3538..6588)
                     /gene="fdnG"
                     /locus_tag="OO249_003505"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6001..6003),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5637668.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVANYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6782..7615
                     /gene="fdhD"
                     /locus_tag="OO249_003506"
     CDS             6782..7615
                     /gene="fdhD"
                     /locus_tag="OO249_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5637669.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7769..>8460
                     /locus_tag="OO249_003507"
     CDS             7769..>8460
                     /locus_tag="OO249_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCR5637670.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYVDWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKIFH"
BASE COUNT         1996 a         2150 c         2234 g         2080 t
ORIGIN      
        1 gtaacgttgg ttttttctgc tcattattag ctccttctga tgccattcta tttcaggaag
       61 gagtgtccgt taaactcagg ctacctcaga agaggcaatt aaacggctga acaagcttcg
      121 gggctggcgt aaggtttcac gatctgcaat tttacgcgaa gcggttgagc aatatctgga
      181 gcggcagcaa tttccggtgc gtaaagcaaa gggaggcagg caaaaggggg aggtagttgg
      241 tgttgatgat cagtgtaagg agcataagga atgaaaaaaa tttttcattc ttgaatataa
      301 aaaaacagat gcccttattc tggtattaat acaaggctgt tttacttgaa cttataataa
      361 ctgcaactgt tacatcgtat ctggaaaacg cctcgcaaaa ataaaaaatg atgcgtaaat
      421 gagctttctt taaagattcg gcaagggtac ttttctctcc atagattcaa aaatgatatt
      481 ggcgagatat ttatgaactc tctggcaggt atagatatgg gcagaatttt actcgattta
      541 tcgaatgagg tgattaagca acttgatgat ctcgaggtgc agcgtaatct tcctcgtgcg
      601 gacctattaa gggaagcggt agatcaatac ctgataaatc aatcgcaaac agcaagaacc
      661 agtgttcctg gcatctggca agggtgtgag gaagatggtg tcgaatatca gcgtaagctg
      721 cgcgaggaat ggtaatggga aaaaatgatg tcaatcagat tgctgacaac gtgcgcgttg
      781 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aagtcatgca aattcaatat
      841 attgaagttt attggtaggc ctgataagcg cagcgcatca ggcaatcata cgcttgtcat
      901 cagtgacata cccataacct tatcgatgtg ttggagggaa gaacgaggca aacagacaat
      961 tgcggcatga tcttgaatgg gtaacgcgga atacgagaga tggtgtcgtc acaccctatg
     1021 agctgtaatc cgggcgacca gcgccacccg gaaaacgtca gagattactc cccttcaccc
     1081 ggaaacagga acgggttgat ggaactgcgg gcatagcctt cttgctccat tcgcgcgtcc
     1141 agtaccagag aagcgaggtc atctgccacg gcttcaactt tcggttcttt ttcctgataa
     1201 agaatcttca ggtaagtgcc gcagtcatcg cagctttcgg ctttaatcgc ggcctgttcg
     1261 tcatccagcg accagtaatg cagtttgccg ctctgttcac agttgctgca ttttacgcgc
     1321 actacgtgcc attcggtttc acacaggttg cagtgcaggt aacgcagacc ctgagtggtg
     1381 ccaatttgca ccatgctgga caccggcata gaaccacaaa ccgggcaata ttgacgttgc
     1441 tcgccgtatt cagcgcgggc tttgccgggg atcagattgg ccatctgcgc ccagtagagc
     1501 gacagtgcag cccagataaa cggcgcttta tcgctgctga cggatgagaa atcagaggca
     1561 aacagtgcgc tggccatatc ttccagctcc tgagtcgatg ccttctccag attctcaatc
     1621 actgccagcg ccgggccgct catttcaggt ttcagctcag caatcagcgc catcagcagc
     1681 ttttgccagt gcttatcacg cggcagaacg tgaatatcca gcgggggctt gccctgtgcg
     1741 ctggcttctt taatgcgcgc ggtcagatcc atctccagcg gatggtcgta cagcaccact
     1801 tcctgggcgt gggcgataag cgcagcaaag cgcaggtaat catccagcgg attattttct
     1861 gccagctcgc gcagacgctc ggcgcggcgg ttgtataaat tcttgagccg agggaacaat
     1921 aacggcggaa tcatatccgc cgtacgtttc tcgctcgaac ccagctcatc ttgcgggatt
     1981 atgcgaatac tcattcagct ttcttttccg ttgtcttgcg gacctcacgg taccagcgcg
     2041 ggtgatgttt cttcgcccag gcgctggtaa cccatccttc caccatggcg gtaatcgtgc
     2101 ctttcaccca aagggcggcg tagatatgca ccatgataac cacaattaac gccactgcgg
     2161 caaatgaatg cagcattaac gcgaatcgga tcaccgggat tgagaaagca ggcgcaaaat
     2221 aaggacgcca gataatcacg ccgctcacca gcagcagaac caggaaaata atcgccgccc
     2281 agaaaacgca tttctgaccg aagttataac gcccggtgtc acctacttcc tcgttgacga
     2341 cgatcttacg aatattcttc gcccaaaaga tatcatcccg attgattagg ttgtgatgcc
     2401 agtaacggaa aaacatgatg atgaacgagg caaacataac cacgccgaca aacgggtgca
     2461 gaattcgcgc cagctgcggt gtgcccatga tttgcatcaa ccagttgaag gacgggaaca
     2521 aaaagcccag cccgctcacc gccgctagga tgaagcagaa ggcggtgatc cagtggttga
     2581 tacgttccgg cgcggtgtag cgcacgatgg tgtcacgtcg tttcatttgc gctcctcgtc
     2641 tttctcttcg tgcagattat tctcttcctc atccgcacgg ttcggaccga cacccacgta
     2701 gtggaagata ctggctgcga aggtagccgc aaagccaaca gctgcgagcg gtttccagat
     2761 gcctttccag aatttcacgg tttcgctgat ttccgggttc tccggcaagc catgatacag
     2821 atttggcttg tcagcatggt gcagcacgta cataacgtgt gtaccaccga cgcctgccgg
     2881 atcgtacagg cccgcattgt cgtaaccgcg ggttttcagc tccgccacgc gctcgctcgc
     2941 cagcgttttc atcgactctt tcgtaccgaa gtgaatcgcg cccgttgggc aggtcttcac
     3001 gcaggctggt tcttgcccaa ccaccacgcg gtcaacgcac agcgtacatt tgtagacgcg
     3061 gttgtcttcc gggttgaggc gcggaatgtc gaacggacag cccgcaatgc aataaccgca
     3121 gccaatgcac tgctcggact ggaagtcgac gataccgttg gcatactgaa taattgcacc
     3181 ttccgccggg cacgctttca gacagcctgg atcggaacag tgcatacagc cgtctttgcg
     3241 gatcagccat tccagtttgt cgttctgctc cacttccgag aagcgcatca ccgtccacga
     3301 tttggcactt aaatcattgg ggttgtcgta caccccaatg ttattgccga cggtatcgcg
     3361 aatgtcgttc cactctgaac acgccacctg acaggcttta cagccgatac aggtggtgac
     3421 gtcgatgagt ttcgccactt cttcctggaa gtcccgcgcc tgaggcgcgg gggtcagacc
     3481 gttagtcgcg gaacgacgaa tgatatcttg agattgataa gccatatgtc gtctccgtta
     3541 caccttttcc acattcacaa ggaaggactt aaactccggc gtctgcgtgt tcgcatcacc
     3601 aacgaatggc gttaaagtat tggcaataaa gcctttttta gctacgcctt catagcccca
     3661 gtgaatcgga ataccgatgg tatcgatatc tttgccgttc gctttcagcg tgcgaatacg
     3721 tttggtcacc accgctttgg ctttgatata gccacggttg gaggagactt tcacggtatc
     3781 gccctgggca atgccaagtt tattcgccag cgactccccg atttccacaa actgctctgg
     3841 ctgcaaaatt gcgttcaaca gcgcgtgttt ggtccagtag tggaagtgct cggtcagacg
     3901 ataggtggtt ccgacatacg ggaacttatc ggctttaccc aatgcttcgg cgtcgtcttt
     3961 aaagatacgt gcagccgggt tcgagataac gtttggatgc agcgggttag ttcccagcgg
     4021 cgtttcaaac ggctcgtagt gttccgggaa cggaccttcc gccatcttat cgagggcaaa
     4081 cagacgcccc atgccttcct gctgcatgat aaacggcccg acgccgctac ccggaggcgc
     4141 tgcgctgtag tccggaatat cccagccggt ccacttagtg ccgtcccatt tcagcaactg
     4201 acgcttcgga tcccacgggt taccctgcgg atctgcggag gcgcggttat acagaatgcg
     4261 gcggttaagc ggccatgccc atgcccagcc cagcgtgtta ccgaggccag acggatcggc
     4321 gttatcacga cgcgccatct ggttgccttc cggcgtccag ctaccggcga aaatccagca
     4381 gccacaggag gttgtaccgt catcgcgcaa ttgggcgaac gagctaagtt gttggccttt
     4441 cttgacgata accgcaccgg ttgccggatc ggtaatatcg gccagcgcct taccgttgct
     4501 ctccatcgct acttcttccg acttcggctc atgcggaatg gcgtagttcc aggtcatgtt
     4561 cagcacctga tccgggttcg cgccaccctg ttcggcatac atcttgcgca agcgcaggaa
     4621 gataccggag aggatctcac cgtcagtcag cgcaatcccc ggggcgtccg cacctttcca
     4681 gtgccactgc aaccagcgac ctgagttaac gattgaaccg ttctcttctg cgaagcaggt
     4741 cgatggcaga cggaacactt cggtctggat cttcgacgag tcaacttcgt tcagctcacc
     4801 gtggttctgc cagaagttag aggtttcagt gttcagcggg tcgatggtga cgaggaactt
     4861 cagtttcgac agacaaccga tcactttgtt tttgttcggg aatgaggcaa cagggttaaa
     4921 accctggcag atatagccat tgaccttgcc ctctttcatc atctcgaagt attgcaggac
     4981 gtcgtagcct ttatcccact tcggcaacca gtcaaagccc cagctatttt ccgccgtcgc
     5041 tttatcacca aagaaggcct tcatcataga gacgaagaat ttcgggtagt tgccccagta
     5101 gtttacctgg ccttccagca gcggttttgg cgtgttggcg gtaaggtagg tttgcagatc
     5161 ggtctgcttc tcgcttggca gcgtcatgta acctggcagg ctctgcgaca gcagccccag
     5221 gtccgtcagc ccctgaatat tggagtgacc gcgcagggcg ttaacgccgc cgcctgccat
     5281 ccccatattg ccgagcagca gctggatcat cgccatcgta cgaatgtttt gcgcaccaac
     5341 ggagtgttgc gtccagccga gggcatacag gaacgaggca gttttatcgt gagcactggt
     5401 ttctgcgatg tattcgcaga ctttcaggaa cgcgtctttt ggcgtaccac agatgttttc
     5461 aaccacatct ggcgtgtagc gggaaacgtg ctgtttcagc aagttccaca cgcagcgcgg
     5521 atgttgcagc gtggtatcgc gtttggcgaa gccgttttcg tccagttcgt aagtccagga
     5581 ggatttatcg tacttgcgtt tttccgcgtc atagccggtg aaaaggccat cttcaaagcc
     5641 gtaatcctca cgcacgatca ggctggcgtt ggtataggct tcggtgtatt cgcggttgaa
     5701 tttttcattg ttcagcaggt acagcaatac gcctgacagg aaagcaatgt cagtaccgga
     5761 acgaataggg gcatagtagt tagctaccgc cgccgtacgc gtaaagcgag gatcgatcac
     5821 aatcagcttc gcgccgttgt gaattttggc ttccatcgcc cagcggaacc cgaccgggtg
     5881 agcttcagcg gcgttaccgc ccatcaccac gacgaggttg gcgttcttga tgtcgaccca
     5941 gtggttggtc atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg
     6001 tcagacacgc gcctggttgt cgaccgcgag catacccagc gcgcgggaga atttttgcgt
     6061 taaatagccg gtttcgttac tcgacgcgga agcacacagc atcccggtgg agagccagcg
     6121 gttaacagtc acgccttcgg cgttttgcgc aatgtagtta gcatcgcggt cttctttcat
     6181 cagtttggcg atgcgatcaa acgcctcttc ccaactgatt tgctgccatt tatcagaacc
     6241 tggcgcacgg tattccggaa acttcagacg gctttcggag tggatgaaat ccaccaggcc
     6301 agcgcctttc ggacaaagtg caccgcggtt gaccgggtga tccggatcgc cttcgatatg
     6361 gaagatagat gctttggcgt tttttgctcc gtcaccgagg ctgtacatca acagcccaca
     6421 gcctacggaa caataggtgc aggtattacg ggtttcgcgg gtgcgcagca gtttatactg
     6481 ccgggtttcc gcgagtgcta cgctgggtgc aaaacctagt gccgctgccg tggtgcctgc
     6541 cataccgcca gcgcagatct taaagaactg ccttctgctg acctgcatgg attgctcctt
     6601 gtttcgacat tgtcacgtcc catttacatt cgcttgctgc gtgtgcaggg agtgggagtt
     6661 atttttcttt gcggaagggg ccgcaaaggt ccagaattgg ctcgatttcc ctccatccag
     6721 gaagggtttg taacagaata ccataatgtt ggtgtgtgtg ttcttatctg gttaagagaa
     6781 agtgaaaaaa acacagcgaa aagaaatcga aaatgtgaca aatatcacag gtgttcgtca
     6841 aattgagtta tggcggcgtg atgatttaca acacccacgg ttagatgagg tcgcggaaga
     6901 agttcccgtt gcgctggtct acaacggcat ttcgcatgtg gtgatgatgg cgtcgcccaa
     6961 agaccttgag tactttgcgc tcggtttttc gctttccgag gggattatcg aaagtccgcg
     7021 cgatatcttc ggcatggatg tcgttccttc ctgtaatggt cttgaagtac aaattgagct
     7081 ttccagccgc cgctttatgg ggttgaagga gcgccgccgg gcgctggcgg gacgtacggg
     7141 ctgtggcgta tgcggcgtgg agcaacttaa tgacatcgga aaaccggtgc agccgctacc
     7201 gttcacccag acgtttgatc tcaacaaact ggatgatgca ttacgtcatc tcaacgattt
     7261 tcagccagtg gggcaactga ctggttgtac tcacgccgct gcctggatgt tgccatctgg
     7321 cgaactggtc ggcgggcatg aagacgtggg tcgccatgtg gcgctggaca aactgttagg
     7381 ccgccggtca caagaagggg aaagctggca gcaaggtgcg gtactggttt ccagtcgtgc
     7441 cagttatgaa atggtgcaaa agtcggcgat gtgcggcgta gagattttgt ttgcggtgtc
     7501 tgccgcgacc acgcttgctg tagaagtggc tgagcgctgt aatctgacac tggtaggttt
     7561 ttgtaaaccg ggtcgggcaa cggtttatac ccatccgcag cgtttgagca attaattaaa
     7621 ataagtcctg cgatatatat taaattgcag gacttattca tttcgtgaat tttattattt
     7681 ttatttataa ataaaacgcg tgtagtggcg ttttcttttt tcatttatta tgttcactct
     7741 cttatctata tatttataag gcaattaaat gaaaaggaat ttattatctt ccgctattat
     7801 agtcgccatc atggccctcg gtctgacggg ttgtgatgat aaaaaagccg aaacagaaac
     7861 gcccccgcct gccaatagtc aacctgccgc accagctcct gaagctcccg ttgcaaaagc
     7921 agaagctaaa cctgaaacac ctgcgcaacc ggtggtcgat gaacaagcgg ttttcgacga
     7981 aaaaatggat gtctatatca agtgctataa caagttacag atccctgtac agcgcagtct
     8041 ggcgcgttat gttgactggc tgaaagattt taaacagggg cctaccggtg aagagcgtac
     8101 tgtttatggc atctacggca ttagtgaatc caacctcgct gagtgtgaaa aaggcgtaaa
     8161 aagtgctgtg gcgttaacgc ctgcgctgca accaattgat ggcgtggcgg tgagttatat
     8221 cgatgctgcc gtggcgttgg gtaataccat taacgaaatg gataaatatt acacccaaga
     8281 aaattataaa gacgatgcgt ttgcgaaagg gaaaacgctg caccagacat tcttaaaaaa
     8341 tctggaagcc tttgaacctg tagcagaatc ttatcatgcg gcgattcagg aaattaacga
     8401 taagcgccag cttgcggaac tgaaaaatat tgaagaaaga gaagggaaaa tattccacta
//
LOCUS       DAJTOR010000175         8303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTOR010000175 DAJTOR010000000
VERSION     DAJTOR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.175"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(96..1766)
                     /gene="hscC"
                     /locus_tag="OO249_003508"
     CDS             complement(96..1766)
                     /gene="hscC"
                     /locus_tag="OO249_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR5637671.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEIHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKQEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(1850..2785)
                     /gene="rihA"
                     /locus_tag="OO249_003509"
     CDS             complement(1850..2785)
                     /gene="rihA"
                     /locus_tag="OO249_003509"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5637672.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTDNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(2903..3628)
                     /gene="gltL"
                     /locus_tag="OO249_003510"
     CDS             complement(2903..3628)
                     /gene="gltL"
                     /locus_tag="OO249_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5637673.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(3628..4302)
                     /gene="gltK"
                     /locus_tag="OO249_003511"
     CDS             complement(3628..4302)
                     /gene="gltK"
                     /locus_tag="OO249_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5637674.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNIFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(4302..5042)
                     /gene="gltJ"
                     /locus_tag="OO249_003512"
     CDS             complement(4302..5042)
                     /gene="gltJ"
                     /locus_tag="OO249_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5637675.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            complement(5212..6120)
                     /gene="gltI"
                     /locus_tag="OO249_003513"
     CDS             complement(5212..6120)
                     /gene="gltI"
                     /locus_tag="OO249_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5637676.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            6575..>8303
                     /locus_tag="OO249_003514"
     CDS             6575..>8303
                     /locus_tag="OO249_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR5637677.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGAGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYV"
BASE COUNT         2071 a         2050 c         2076 g         2106 t
ORIGIN      
        1 tatgctctcg gcaaaatcgc gatgttgttt agttgacgat gtgttttttg cgccgatgcc
       61 ggatgcagcg tttacgccgc atccggcagc cgtgtttatg ggatctcaat ggctaaataa
      121 tcgctcgccc gccgccgaac ttcatccacg cgggccatgt cgtttgactg tagcgccgca
      181 tcaaaatcgg tgatcacccg gccaatctct tctcgctcgt cacccagcgc tcgcgcccac
      241 aactcttcct gtttggcttt aaaggtgcga ttgatgagca tatcgcgcgg ataaattttc
      301 aatgcggata accgcgtccg actctcttca atctgctgcg cgctcaatgt taccgggctg
      361 tggttaatca ctctggactt aacgctgccg tcttccagaa gcacgtcaac ttcaagcaac
      421 ccgttgatat cataactaaa gcgaatatca atcgactgat aagccccggt tttcttcaac
      481 ggcacatcga aggattcaac cagaatgtta tttttaactt tgtggttttc tccctgatag
      541 acgttaaccg taattgaatc ctgctccggg tgcatggtcg agtaagtttc tacacgcgac
      601 accggtactg tggtgtttcg ttcaataatc ggcgagaaaa tgccggaaac gccctggcgg
      661 ttaacttcaa cgcccaacga gtaagggcaa atatcagtga ggattacctc ttcaatatct
      721 tcactgcgta agcggcaggc ggcctggatt gctgcgccca gcgcgacaat ggtgctcgga
      781 tcgtaacttt gatacggtaa tttgccaaac agacgcacgg cgattcgctg caccagcggc
      841 atttgtgacg cgccgccaac cagcaccaga ctgtcgattt gactcggctt cagacgcgca
      901 tcgcgcaacg cctgttcaat cggcactcgc aagcgattga gcagcggcaa ccacaaatct
      961 tccagttcgt tctcgtaaaa ttcgcattcc cgcgtttctt cctgatactg ccagcgaatg
     1021 tgcaatggcg attgattgct acattttgcc gcttccacac aggcgtacaa ggctgccagt
     1081 tcactctcgt taagcgtggt cctggcaact tccgcgcgtt ttaaaacctc atcgaccagc
     1141 atatgggtaa aatcttcgcc accgaggaag ctatcgccag cggaggcgtg aatttcaatc
     1201 accggcgtgg cgtactcaag caccgtaacg tcaaacgtgc cgccaccgag atcaaaaacc
     1261 agcgagcggg tattttgttg cgtatgcagg ccatatgcca tcgcagctgc tgtgggttca
     1321 ttaattaagc gtaccgcatt taacccggct aactccgcag ctaaacgggt atgcttgcgt
     1381 tgttcatcgc tgaaataagc cggaacggag atcaccacat ctttaatcgg acgttgcaga
     1441 aattcttcgg catcttcttt taatgagcgt aataccaaag aggacagttc tggcgcgtta
     1501 aatgtgtcgc tgcctaaccg ccagttggta ttactgccca ttgcgcgttt aaataacgct
     1561 gccgttttat ccggatgcga agtacgccgt gatacagccg gttttccgac taaaatatga
     1621 ttattttcat ccatgctaat tatggatggt gttaaatatt caccgaactt atttggaatt
     1681 aattgcgcgg caccgtcttt ccagacggca attaaactat tggtagtgcc gagatcgata
     1741 ccaatggtga gttctgcatt atccattgca tttatatctt gaaagaaaaa gagataaaca
     1801 gattaagacc acccaggtaa cgcgtggtga tcttaatcaa tgacgtgtgt taagcgtaaa
     1861 atttcagacg atcagccagt aaatcaacaa agccctgacg atcaacatcg accattacag
     1921 tggcattcgg tttattgtca gtcagataat aataatcaac aaccgtcata ccctgggtat
     1981 atttcccctg tgtttccacg ccaacccagc gctcaacagt ggtaaacaac tcgggtttca
     2041 acagccaggc gatggtgcat gggtcatgca gcggtgcacc gacaaagccc catttttcgt
     2101 ctttatgata ttcgaggaag aaatccagca gttcggcaac aatggttgaa acagggttgc
     2161 caatcgcgcg gaaacgctcg gtgtcttcaa tgtggatttg cgctttatga gtaacatcca
     2221 gaccggccat caccactggg atccctgact ggaagacaat ttctgccgct tccgggtcaa
     2281 cgtaaatatt aaattcagcc gcaggcgtcc agttaccaag ccccattgcg ccgcccataa
     2341 tcacgatacg ggcaattttg ctatgcagtt ccggatggct attgagcagc aaggcaacgt
     2401 tagtttgcgg tccggtagac acaatcgtga caggttccgc actttcgcgc agcgttttcg
     2461 ccatcagctc taccgccgta cagttttgcg gtgcgaacgt cggttccggt aatgccgggc
     2521 catccagacc gctttcgccg tgcacattgt ccgcgataat caactcacgc attaacggtt
     2581 ttaccgcgcc gcctgctacc ggaatatcgg tgcgattaag caaggtcagc atacgcagaa
     2641 cattgcgtaa ggttttttct ggtgtctggt ttccggcgga agacgtaatt gctttgacat
     2701 caagctctgg tgaggcgagg gcgagaacta ttgcgatagc gtcgtcatga cctgggtcgc
     2761 aatctaacag aattggcagt gccattgttg ctccttgttg tgtgcttctt tgcgacaagg
     2821 gtaacgccag gatgtaacag atacgagggg cgaaacgata aagcgtgaga tggcgcgcac
     2881 ttgggtatgc gcgccagagt gattaatgca ggattttcgc gaggaagtct ttcgcgcggt
     2941 ccgatttcgg atcatcgaag aaagcgtctt tcggcgagtc ttcgacaatt ttaccctcgt
     3001 ccataaagat cacccgattc gccactttgc gggcaaagcc catttcgtgg gtcaccacca
     3061 tcatggtcat tccttcgttc gccagttcca ccatcacgtc cagcacttcg ttgatcatct
     3121 ccggatccag cgccgatgtc ggttcgtcga acagcatcgc aataggatcc atacacaacg
     3181 cgcgagcgat tgccacacgc tgctgctgac cgccggaaag ctgcgccgga aatttattgg
     3241 catgtgcgga aagtcccacg cgctccagca atttcaatgc tttttcacga gcgggcgctt
     3301 tatcgcgttt aagcactttc acctgcgcca gagtcaggtt ttcaataatc gacaaatggg
     3361 ggaacaactc aaaatgctgg aacaccatcc cgacgcggga acgcagcttt gccagatcgg
     3421 ttttcttgtc attaaccacg ataccatcga cggtgatttc accttgctgc actggttcga
     3481 ggccgttgac ggttttaatc agcgttgatt tgccggaacc agacgggccg caaaccacca
     3541 ccacttcgcc ttttttcact tcggttgagc agtcggtcag cacctgaaag tgaccatacc
     3601 attttgaaac atttttcagg gtaatcatta tgctgtcctt cttttcaagt agctgaccaa
     3661 caacgacgcg ctaagactaa taacgaaata aacaaatccg gcaaacagga tcatctcaac
     3721 ctgcgtacca tcacgctcac caatggttga ggcggtacgg aagaaatcgg ccagagataa
     3781 cacatacacc agtgaggtat cctggaacag tacgatgccc tgagtgagca gaagcggcac
     3841 catcgcgcgg aacgcctgcg gcagaataat cagtttcatc gactgccagt gagtcattcc
     3901 caacgccagc gcggcgctcg actgaccacg agaaatactt tgaataccag cacggataat
     3961 ctctgaatag taggccgctt caaacatcga aaacgccacc atcgccgaaa ttaaacggat
     4021 atcatttttt ggtgataatc ccagcacgtt ttgcagaaaa cctggcacga tcaggtaaaa
     4081 ccacagcaaa accataacta aaggaatcga gcggaatatg ttaacgtagg ctttggcaaa
     4141 ccacgctacg ggcgcaaagc tggacaagcg catcaccgcc agcatggtgc cccacaaaat
     4201 accaatcacg actgccgtga cggtgatttt cagcgtgatc accagcccgt cgagcagata
     4261 tggcagggaa gggacaatgg aactccagtc aaactcgtac attatttgcc ccccatgttg
     4321 ccaggcaggc gaactttacg ttcaaccagc gtcattacca gcatgataat cgcgttaatc
     4381 aacacatacg ccagcgtaat agcggtaaac gactcccagg cgtgggctga gtaatcaagc
     4441 aatttacccg cctgcgccgc catatccacc agaccaatag tcgaggcgat ggcggagttt
     4501 ttcaccaggt tcatcatctc ggaagtcatc ggcgggacga taacgcgata agcattaggc
     4561 agcagtacgt atcgataagc ctgcggtagc gtcaggccca tcgccagcgc ggcatttttt
     4621 tgcccacgcg gcaacgactg aatggcggcg cgtacctgtt cgcaaacacg ggcggcggta
     4681 aacagcccca ggcagagcat ggatgaaagg aaaaactgaa tattgggatc cagctcggcc
     4741 ttaaaccaca tgccgatttt ctccggcaac agctccggga tcaccagata ccaggtaaag
     4801 aattgcacaa tcaacggcac gttgcggaac agttcgacat acaacgtacc cagaccggag
     4861 agaaaacggt ttggaacggt acgtaaaatg ccgaaaaatg aaccgacgag gaaagcgata
     4921 atccaggcgc agatcgacaa agcgatcgtc acctgaaaac cactccagat ccaaccaaga
     4981 taggtggtgt tgccgaacgg ggcttgttgt aaaaaaatac cccagttcca gtctatagac
     5041 ataaatctac tccagaaaaa agagggtagc agcgttaact gctaccctcg aagattgtta
     5101 cccagcgtat tgcggttttc aggcccgatg gggaacgacc atcaggcgta tagtctgtcc
     5161 gtgctacgta acaatcgaga gggctggaat ttccgcccct ggttcttgta attagttcag
     5221 tgctttgtca ttcggttctt tgaacagtgc tttcatttcg tctgacagtt cgaaattcat
     5281 gttcaggttt ttcggcggaa ttggattttt gaaccattta tcaaaccatt tttccgcttc
     5341 accggaagtc tgcacctgag cgatagtgtc atccatcagc tttttgaact gcggatcatc
     5401 tttacgcaac atgcaaccgt aggcttcctg agactgcggc ttgccgacga tatcccagtt
     5461 gtctggtttc ttcgctttcg cacgttcacc ggccagcagg gcatcatcca tcataaaggc
     5521 aacggcacga ccgctttcca gggtgcggaa agagtcaccg tgatctttgg cgctgatgat
     5581 gcgcatattc attttttgct cttcattcag tttgttgagc aaaacttcag aggtagtgcc
     5641 ggaagtgacg actacggctt tgtctttcag gtcggcaaaa tctttgatat cgccgccctt
     5701 tttggtcagc aggcgcgtac cgaccacgaa aatagtatca gagaaagccg cctgtttttg
     5761 gcgttcgacg ttgttggtgg tagaaccaca ttcaaaatcg aaagtgccgt tttgcagcag
     5821 tggaatacgg ttttgtgagg taatcggaat cagttttacc tgcaagtccg gtttgttgag
     5881 tttctttttc actgcttcaa caatggcgtt ggagtaatcc tgcgagtaac ccaccacttt
     5941 ttgctgattg tcgtaataag agaaaggtac tgaagattca cggtgaccga cgacaatcac
     6001 accgtttttg gcgattttgt ccagcgtact gcctgctgcc ggggcggcgt catctgcctg
     6061 tgccagtcct gcggaaagcg ccagggcgag gattgctgtg gcaggtttac gtaattgcat
     6121 atccaactcc tttatcttct gcgttaagaa cgcatggata cccgttgtga gtgtttgtgt
     6181 tgttatcgtc tgcaacttta ttgtgcagtg ttgtgcctgt taggtaacat ttagtttggc
     6241 taaatgtaaa gatattgctg ttttattgtt tgtttttgcg agatgcgccg caccattccg
     6301 aagcaaaatt cttaaaccgc acctttttgg tgctaccgct gatttgtggt ggtgcaacct
     6361 ggttgcgtcg cggctgattt catcgcttac acagtaaata aagcaactta catgccaaaa
     6421 aatacttagg ggcaaattaa ttacgattgt aattttaggc tgaaaaagtc atatttcgga
     6481 cagaattatc ttttcggcat gggacgtttg ccgggatgat aactgcacac gggaaaaatg
     6541 ctttttacct gtcttttcaa ggaagaaaca ccttatgtct gcatcttcgg ttaagccgtt
     6601 aaatgttcaa ttacccgcaa taacccttat cctttttgca ctctgtattg ggatattttg
     6661 ttatctcgca caatggatga gttatgaaga ggtcgatcaa tccgcactta tccatctcgg
     6721 cgcaaacgtt gcgcctctta ctttgtcggg tgaaccctgg cgcttattga gcagtatctt
     6781 tctgcacagt agtgtttctc atctgctgat gaatatgttt gcattcctgg tcgtgggagg
     6841 cgtggcggaa cagattctgg ggaaatggcg actcctgatt acctggttat tctccggcgt
     6901 ctttggtggg ctcatcagcg cctgttatgc gttacgcgat agtgatcaga tagtcatcag
     6961 cgttggggca tccggggcaa ttatgggaat agctggcgct gcgatagcaa cacagcttgc
     7021 ttcaggtgcg ggcacatacc ataaaaacca gcggcgagta ttttctctgt tgggtatggt
     7081 ggcgctgaca ctgttgtatg gtgcccggca aacaggaata gataatgctt gtcacattgg
     7141 cggcctgatt gcgggtggcg cgttgggttg gctgagcgcg cgtttatctg ggcaaaaccg
     7201 actcgttacg gaaggcggga ttattgttgc gggcagtctt cttctgaccg gggctatctg
     7261 gcttgcgcag cagcagatgg atgagtcagt tttacaagtc aggcaaagcc tgcgtgaagc
     7321 gttttatcca caggagattg aacaagagcg acggcaaaaa aagcagcagt tagcggagga
     7381 acgcaacgcc ctcagggaaa cattatccgc tccggtaagt cgtgaacagg ccagtggtga
     7441 tttgctcgct gagattgccg atatccatga tatggcgatc agtcgggatg gtaatatgtt
     7501 gtatgccgca attgaaaaca cgaacagcat tgttgttttc gacctcggac aaaagaaaat
     7561 cctgcatacc tttacagcgc ccatagcgaa agaaaagtca gtcaaacatt gtggtggctg
     7621 taaagatcag ggcgtcagat cgctggcatt aagcccggat gaaaagttga tttatgcgac
     7681 ttcatttgaa gcgaatgcgt tatcggtcat taacgtggcg acaggggaga ttattcagtc
     7741 gattaccacc ggtgcacatc ctgacagcct tatcctctcg cgtgatggca caaaagcctg
     7801 ggtgatgaat cgcaccagta atagtgtgtc agcgattgat ctggtgactt atcagcatgt
     7861 ggcggatatc ccgctggaga aatacgacgg ggcggggacg agcggtaaac caggcgcctg
     7921 ggttatggca ctttccccgg atgaaagaac attactggtt ccgggagcag gcagaggtaa
     7981 catcgtgcgg atcaatacca tcacgcatca gaaagaagac tttcccgcag gtgatgcgcg
     8041 tggaacgata tcggcgatgc gttttcgacc tgaaaacggc gaggttattt ttgcagatag
     8101 tcaggggatt tcacgtataa gcgtagggga tcaacaagcc agcattatga cgcaatggtg
     8161 tagcaggagc gtttattccg ttgagggtat tagcccggac ggtcaatatt tagcgttggt
     8221 gtcatatggc ctgcaaggtt atgtcatcct gctcaatatt aatgccgggc agattattgg
     8281 cgtttatcct gccagctacg tta
//
LOCUS       DAJTOR010000176         8204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTOR010000176 DAJTOR010000000
VERSION     DAJTOR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.176"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..825)
                     /locus_tag="OO249_003515"
     CDS             complement(<1..825)
                     /locus_tag="OO249_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5637678.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDY"
     gene            917..1003
                     /locus_tag="OO249_003516"
                     /pseudo
     CDS             917..1003
                     /locus_tag="OO249_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1304..1393
                     /locus_tag="OO249_003517"
                     /pseudo
     CDS             1304..1393
                     /locus_tag="OO249_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            1417..5931
                     /locus_tag="OO249_003518"
                     /pseudo
     CDS             1417..5931
                     /locus_tag="OO249_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            5932..7581
                     /locus_tag="OO249_003519"
     CDS             5932..7581
                     /locus_tag="OO249_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5637679.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIHTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            7586..>8204
                     /locus_tag="OO249_003520"
     CDS             7586..>8204
                     /locus_tag="OO249_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637680.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQV"
BASE COUNT         2003 a         1971 c         2304 g         1926 t
ORIGIN      
        1 gtagtccggc gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt
       61 gttataaata ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt
      121 cagctgattg ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg
      181 tagttggttt tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg
      241 taatctgtta tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt
      301 aagctggttg ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc
      361 caggctattg agaagggctg agagtgactg caaggaagat ggaagagaag gtaaacatgt
      421 taaaactctg ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct
      481 gctcagtctg ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga
      541 gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga
      601 gcgtaatctg agtaaactta aattgagggc tggctcttga aaagccagac atattttcag
      661 taatcgaact gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca
      721 gatgcggtga taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa
      781 accacaaaag gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc
      841 attgtttata tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgctcc
      901 tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt
      961 gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt
     1021 catcaagcca gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag
     1081 catggttgta aattcaacta acccagtgcg ttgacaaaaa attagcgcaa gaagacaaaa
     1141 aatcaccttg cgctaatgct ctgtatcagg tcactaatac cagctaagta gttgattcat
     1201 agtgactgca tatgttgtgc tttgtcgcat tttgtagtct attatttaat ctgtaacaac
     1261 gataatacaa tgaattttaa ttggtttttg tgattttgtc cttcttctga catggaagga
     1321 cacccgatgg atacccaacg attccaatcc caatttcatt ggcatttatc gtttaaattt
     1381 tctggcgcga tagcagcgtg cttgtctctg tctcttgtgg gaacagggct tgctaatgct
     1441 gatgattcgc ttccttccag taactatgcg ccgcccgccg ggggaacatt ctttttgctt
     1501 gctgacagca gttttagcag cagtgaagag gcgaaagtgc gactggaagc gccggggcgt
     1561 gattatcggc gctatcagat ggaagagtac ggcggcgtgg acgttcgcct gtatcgtatt
     1621 cctgacccga tggcattttt gcgccagcag aaaaacctgc atcgcattgt ggtgcaaccg
     1681 caatatctgg gcgacgggct gaacaatacg ctgacctggc tgtgggataa ctggtacggc
     1741 aaatctcgcc gcgtaatgca gcgtactttc tcttctcagt cacggcagaa tgtgactcag
     1801 gcattacccg aattacatct cggcaatgcc attattaaac cttcccgtta tgtacagaac
     1861 aaccagtttt ccccgctgaa aaaatatccc ttggtggaac agttccgtta tccactatgg
     1921 caggctaaac cggtcgagcc gcagcaaggg gtaaaactgg aaggcgcatc cagcaatttc
     1981 atctcgccgc agccgggtaa catttatatt cctctcggcc aacaagagcc gggactgtac
     2041 ctcgtcgagg cgatggttgg tgggtatcgg gcgacgacgg tggtgtttgt ttccgatacc
     2101 gtggctctta gcaaagtgtc aggcaacgag cttctggtgt ggaccgcggg taaaaaacag
     2161 ggtgaagcga agcccggctc agagatcttg tggactgacg gtcttggcgt gatgacccgc
     2221 ggtgtgaccg atgacagcgg taccttgcag ttacaacata tatcgccaga acgttcatac
     2281 attctgggta aggatgctga aggcggcgtt tttgtctccg agaacttctt ctacgaaagc
     2341 gaaatctaca acacccgctt gtatattttt accgatcgcc cgctatatcg cgcaggcgat
     2401 cgtgtcgatg ttaaagtgat gggccgcgag ttccacgatc cgttgcattc atcccccatc
     2461 gtcagcgccc cggcgaagct ttcggtgctg gacgctaacg gcagtctgtt gcaaaccgtc
     2521 gatgtcacgc tggatgcgcg caatggcggg cagggaagtt tccgcctgcc agaaaatgcc
     2581 gttgccggag gttatgagtt acgtcttgct taccgcaatc aggtctatag cagcagtttt
     2641 cgcgtggcaa actacatcaa gccacatttc gagattggtt tagctctcga caaaaaagag
     2701 ttcaaaactg gcgaagcggt cagcggcaaa ctgcaactgc tctacccgga tggcgagccg
     2761 gtaaaaaatg cccgcgtgca gttaagtttg cgcgctcagc aattatcaat ggtcggtaac
     2821 gatttgcgtt atgccggacg tttccccgtg tcgctggaag gcagcgaaac ggtgtccgac
     2881 gccagcggtc atgtggcgtt aaatctcccc gccgccgata aaccgagccg ttatttgtta
     2941 accgtctccg ccagtgacgg cgcggcgtat cgtgtcacca ccaccaaaga gatcctcatt
     3001 gaacgcggtc tggcgcatta ctcattaagt actgccgcac aatacagtaa tagcggcgag
     3061 tcggttgtgt tccgttatgc cgcgctggaa tcttcaaaac aggttcctgt tacgtatgaa
     3121 tggttgcgtc tcgaagaccg cacgagccat agcggagagc taccgtcagg cggcaaatcc
     3181 tttaccgtca atttcgctaa acctggcaac tacaatctga cattacgcga taaagacggc
     3241 ttaattctcg ctgggttaag ttatgccgtc agcggtaagg gcagcacggc gcatacgggt
     3301 acggtagata tcgtggcgga taaaacgctg taccagccag gcgaaaccgc gaagatgctg
     3361 attacctttc cggagccaat tgatgaagca ttattgacgc tggaacgcga tcgcgtggaa
     3421 cagcagtcgc tgcttttgca tccggcaaac tggctaacgc tacaacgttt aaacgatacc
     3481 cagtatgaag cccgggttcc agtgagcaat tcctttgcgc ctaacatcac tttttcggtg
     3541 ctgtataccc gtaatggtca gtacagtttt cagaacgccg ggatcaaagt tgccgttcct
     3601 cagcttgata tccgggtgaa aacggacaaa acccattacc agccaggtga actggtcaat
     3661 gtcgaattaa cctcgtcgct gaaaggtaaa cctgtttctg cgcagctaac ggtaggcgtg
     3721 gtcgatgaaa tgatctacgc gctgcaacca gaaatcgcgc cgaatatcgg caaatttttc
     3781 tatccgctgg ggcgtaacaa tgtgcgtacc agctccagtc tgtcgtttat cagctacgac
     3841 caggcgctct ccagcgagcc ggttgcgcct ggcgcaacta accgcagcga gcggcgagta
     3901 aaaatgcttg aacgtccacg gcgtgaagag gtggataccg cggcatggat gccgtcactc
     3961 acaaccgata aacaaggcaa agcgtatttc acgttcctga tgcctgattc gttaacccgc
     4021 tggcgtatca ccgcgcgtgg gatgaacggc gacgggctgg tcgggcaggg gcgtgcttat
     4081 ctgcgttcgg aaaaaaatct ctacatgaag tggagtatgc caacggtgta tcgcgtgggc
     4141 gacaaaccgg cggcaggact gtttatcttc agtcagcagg ataacgaacc ggtggcgctg
     4201 gtgactaaat ttgcaggcgc tgagatgcgc cagacgctga cgctgcacaa aggggcgaat
     4261 tatatttcgc tggcgcagaa tattcagcaa tctggcttgt taagtgcaga actgcaacaa
     4321 aatgggcaag tgcaggacag cattagcaca aaactgtctt ttgtggacaa cagcaggccc
     4381 gttgaacagc agaaaaatgt catgctcggc ggtggcgaga acgcgctgat gttgcccgag
     4441 caggcgagta atatccggct acaaagtagt gaaacgccgc aggagatttt ccgcaacaat
     4501 cttgatgcgt tagtcgatga accgtggggt ggggtgatca acaccggtag ccgtctgatc
     4561 ccgctcagtc tcgcctggcg ttcgcttgcc gatcatcaaa gtgccgccgc taacgacatt
     4621 cgtcagatga ttcaggataa ccgtctgcgg ctgatgcaac tggcggggcc cggagcgcgc
     4681 tttacctggt ggggtgaaga tggcaatggt gacgccttcc ttacggcatg ggcatggtac
     4741 gccgactggc aggccagcta ggcgctcggc gtaacgcaac aaccggaata ctggcagcat
     4801 atgctcgaca gttatgccga gcaggcggat aacatgccgt tattgcatcg ggcgctggtg
     4861 ctggcgtggg cacaggagat gaatctgccg tgcaaaacgt tgttgaaagg gttggatgaa
     4921 gctatcgccc ggcgcggaac taaagatgaa gatttctctg aggaagacat ccgcgatatc
     4981 aatgacagcc tgatcctcga tacaccggaa tctccactgg cagatgcggt ggcaaacgtc
     5041 ttaaccatga cgttgctgaa aaaagcgcag ttgaagtcca cggtgatgcc acaggttcag
     5101 caatatgcgt gggataaagc ggcaaacagc aatcagccgc tggcgcacac ggttgtgctg
     5161 ctcaatagcg gtggtgacgc tacccagacg gccgctattt taagtggttt gaccgctgag
     5221 caatccacta ttgagcgcgc gctggccatg aactggctgg cgaaatatat ggcgacaatg
     5281 cctccagttg ttttgcctgc gcctgcgggc gcatgggcta aacataagtt aactggaggg
     5341 ggcgaatact ggcgttgggt tggtcagggc gtgccggaca ttctctcttt tggtgacgaa
     5401 ttatcgccgc aaaatgtgca ggtccgctgg cgtgagccgg caaaaatggc tcaacaaagt
     5461 aacattccgg tgaccgttga acgccagttg tatcggctta tccctggtga agaagagatg
     5521 agctttattc tgcaaccggt gaccagcaat gagattgaca gcgatgcgct gtatctcgat
     5581 gacatcacgc ttaccagcga gcaggatgca gttctgcgct acggtcaggt ggaagtaccg
     5641 ctgccaccgg gagccgacgt tgagcgcaca acatggggca tttcggtcaa taaacccaac
     5701 gccgcgaaac agcaggggca attgctggaa aaagcgcgaa atgaaatggg cgaactggcc
     5761 tatatggtgc cggtgaaaga actgacggga acggtcactt tccgccattt gctgcgcttc
     5821 tcgcaaaaag ggcaattcgt tctgcctcct gctcgttatg tgcgttccta tgcacctgca
     5881 cagcaaagtg ttgcggcagg gagtgaatgg accgggatgc aggtgaaata agtgaactgg
     5941 cgcagaatcg tctggctatt ggcgttggtt actcagccaa cgcttgcgga ggagacgccg
     6001 ttacaactgg cactgcgtgg tgcgcagcac gatcaactgt atcagctgtc gtcatcaggc
     6061 gtgaccacag tctcagcatt acctgactcg ctcaccacgc cgttagggag tttgtggaaa
     6121 ctgtatgttt acgcctggct ggaggatacc catcaaccgg agcagcccta ccagtgtcgc
     6181 ggaaactccc cggaagaggt ttattgttgt caggctgggg agagcataac gcgcgatacc
     6241 gcgctggtac gttcctgtgg gctctatttt gcgccgcagc ggttgcatat tggcgcagat
     6301 gtgtgggggc aatactggca acagcgtcag gcgcctgcgt ggttagcgtc tcttactacg
     6361 ctgaagccag aaacctcggt aacggttaaa tcgctgcttg attcattagc gacgttacct
     6421 gcgcaaaaca aagctcagga agtcttactg gatgtggtgc tggacgaggc gaaaatcggt
     6481 gtcgcatcca tgctcggtag tcgggtacgg gtgaaaacct ggagctggtt tgccgatgat
     6541 aaacaggaaa tccgtcaggg tggttttgcc ggctggttaa ccgatggtac accgctgtgg
     6601 gttactggca gtggcaccag caaaacggta ctaacccgtt atgcgacagt gcttaatcgg
     6661 gttttgccag tgccgacgca ggttgccagt gggcaatgtg tagaggtgga gctgtttgcc
     6721 cgctatccgc tgaagaaaat cacagcggaa aagagtacga cggcagtaaa acccggcgta
     6781 cttaatgggc gctatcgtgt gacgtttact aatggtaacc acattacgtt tgtcagccac
     6841 ggtgagacga ccttactgag cgaaaagggc aaactgaaat tgcagtcgca tctcgatcgt
     6901 gaagagtacg ttgccagagt tctggatcgc gaggcgaaaa gtacgccccc tgaggcagcg
     6961 aaagccatga ccgtggcgat tcacactttt ttacagcaaa acgccaatcg ggaaggcgat
     7021 tgcctgacca tccccgacag cagcgccacg cagcgtgttt ctgcttcgcc agcgaccacg
     7081 ggggcgcgaa cgatgaccgc ctggacgcag gatctcatct atgcaggcga tccggttcat
     7141 tatcatggca gtcgagctac cgaagggaca ctttcctggc ggcaagccac ggcccaggcg
     7201 gggcagggag agcgttacga ccagatactc gcgtttgctt atcccgacaa cagccttagc
     7261 cgctggggag cgccacgctc aacctgccag ttattaccca aagcgaaagc ctggctggcg
     7321 aaaaaaatgc cgcagtggcg gcgtatatta caaggcgaga cggggtacaa cgaaccagac
     7381 gtgtttgcgg tctgtcgttt agtctcaggt ttcccctata ccgatcggca gcagaaacgg
     7441 ctgtttattc gtaacttctt tacgcttcag gatcggctgg atttaaccca tgagtatctg
     7501 cacctggcct ttgatggtta tcccaccggg cttgatgaga actatatcga aacgctgacc
     7561 cgacaattat taatggacta atgctatgcg aaagattttt cttccgttgt tactggtggc
     7621 actttcgcct gtcgctcaca gtgaaggtgt gcaggaagtc gagattgacg caccgctctc
     7681 tggctggcat ccggcagagg gcgaagatgc cagtttttca caatcgatca attacccggc
     7741 gtcgtccgtc aacatggcag atgatcaaaa tatctcagcg cagatccgcg gcaaaataaa
     7801 gaattatgct gcggcgggta aaattcagca gggccggctg gtggtcaacg gtgccagtat
     7861 gccgcagaga gttgaatccg atggttcatt tgcacgccct tatattttca ctgaaggcag
     7921 caacagcgtg caggtcatca gcccggatgg gcaaagccga caaaaaatgc agttttactc
     7981 aacgccgggt acaggaacga ttcgtgcacg tttacggctg gttctctcgt gggatacgga
     8041 caataccgat ctcgatctcc atgttgtcac gcccgatggc gaacacgcct ggtacggtaa
     8101 caccgtgctg aaaaacagtg gtgcactgga tatggatgtc acgacggggt acggacccga
     8161 gattttcgcc atgccagcgc cagttcacgg acgctatcag gtgt
//
LOCUS       DAJTOR010000177         8166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTOR010000177 DAJTOR010000000
VERSION     DAJTOR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.177"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..657)
                     /locus_tag="OO249_003521"
     CDS             complement(<1..657)
                     /locus_tag="OO249_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR5637681.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     T"
     gene            885..1493
                     /locus_tag="OO249_003522"
     CDS             885..1493
                     /locus_tag="OO249_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5637682.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1591..2982
                     /gene="selA"
                     /locus_tag="OO249_003523"
     CDS             1591..2982
                     /gene="selA"
                     /locus_tag="OO249_003523"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5637683.1"
                     /translation="MTTETRSLYSQLPAIDRLLHDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2979..4823
                     /gene="selB"
                     /locus_tag="OO249_003524"
     CDS             2979..4823
                     /gene="selB"
                     /locus_tag="OO249_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5637684.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFSDEPWW
                     VRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            5013..6164
                     /gene="yiaY"
                     /locus_tag="OO249_003525"
     CDS             5013..6164
                     /gene="yiaY"
                     /locus_tag="OO249_003525"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5637685.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAYQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            6329..7867
                     /gene="aldB"
                     /locus_tag="OO249_003526"
     CDS             6329..7867
                     /gene="aldB"
                     /locus_tag="OO249_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5637686.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT         1904 a         2087 c         2298 g         1877 t
ORIGIN      
        1 tgtgcgcccg aagcggtcca ccagcccggt cagtacccgg tacggcggca gcggcgcagg
       61 cagcacctca tccgcttccg gcacccgctc acaccagccg agcagccacc acggcccctg
      121 cggactgttt gtcgccagat aacgatgcgg acttaagcgg agttcttccg gcagcgcctg
      181 ccagagtgcg gccagccggt gaccttcatc cagtttcgcc acgccgccgc gcaccagcca
      241 cagtgactcg ctgcggctgt aaccgtcctc accgggaaac aggtgctcaa aatacaggct
      301 tctgccgccg ttatcactga gtatcagtgt gttatcgcgc agctgtaagc ggatatccgc
      361 aggcattttc cagccggggc cgaggctccc caccggcgcg ggcgtttttg tccggtaact
      421 gctgtaggtg cgggagagga tgaacggcag cgggccgggc agggcgatgt cggtttcacc
      481 gggaaggacc tttgcaccga gcaggggatt gaccggatgg ccggacgtca ctccgccggg
      541 gcacaccgaa caggccacgc cggtgggggc accaatgcgc accccggctg aaccctgaac
      601 aatgctaccg ccatactgcg tcatgtcgcc ctgacgcgct gccggttttc cgctcatttc
      661 tcatccttgt tttatcgggt tatttgtctg ctgacaggca aaaccctaca gatttataag
      721 aaagcctgaa gtgagatatc catcagagtg aaataattag gaaaatattt atatcaccat
      781 gataaatatg tgatttattt ggaagtaaca aaccatcaac attttctgcc ctttttccca
      841 tcaccaggcc atactgatat ttttcctcgc acaaaggact cggcatgaaa ctcgtcggta
      901 gctacaccag cccgtttgta cgcaaacttt ctattctgtt gttagaaaag ggcataactt
      961 tcgaatttat taatgaactg ccctataacg cggacaacgg cgtggcgcaa tttaacccgt
     1021 taggaaaagt gccggtgctg gtgaccgaag agggcgaatg ctggtttgat tcgccgatca
     1081 tcgctgaata tattgagtta atgaatgtcg ctccggcgat gttgccgcgc gatccgctgg
     1141 agtcgttgcg ggtgcgcaaa attgaggcgc tggcggatgg cattatggat gccgggctgg
     1201 tatcggtgcg tgaacaggcg cgtccggcgg cgcagcagtc tgaagatgaa ttgttacgcc
     1261 agcgggagaa aatcaaccgc agtctggatg tgctggaagg atatctggtc gatggcacac
     1321 tcaaaaccga tacggtcaat ctggcgacta tcgccattgc ctgtgctgtc ggatatctca
     1381 atttccgccg cgttgcgcca ggttggtgtg tcgaccgccc acacttagtc aaactggtcg
     1441 aaaacctgtt tagccgcgaa agttttgctc gcaccgaacc gccaaaggct tgatgcgcga
     1501 tatgtcctcc tgacccatct cacgttacaa tccgtggtta tgttaaacgc ccttctccat
     1561 gtgagagggc cttgattagc caggtttcct atgacaaccg aaacgcgttc cctctatagt
     1621 caacttccgg ctattgatcg cttattgcac gatagctcct tcctttcttt gcgtgatact
     1681 tatggtcaca cccgcgtggt ggaattgttg cgtcagatgc tcgacgaagc gcgagaagtg
     1741 attcgtgaca gccagacgct tcctgcgtgg tgtgaaaact gggcgcaaga agtcgatgcc
     1801 cggttgacga aagaggcgca gagcgcgctg cgtccggtga tcaacctgac gggaaccgtg
     1861 ctgcatacca accttggacg agctttacag gcggaagccg cggtggaagc cgttgcgcag
     1921 gctatgcgtt cgccagtgac cctcgagtac gatctggacg acgctggacg cggacatcgc
     1981 gatcgggcgc tggcgcaact gctgtgccgt attacggggg cggaagatgc ctgtatcgtc
     2041 aataacaatg cggcggcggt gttattgatg ttggcggcca ctgccagcgg gaaagaggtg
     2101 gtagtatctc gcggcgaatt ggtggagatt ggcggcgcgt ttcgtattcc cgatgttatg
     2161 cgtcaggcag gctgcaccct acacgaagta gggaccacca accgcacgca cgcgaatgat
     2221 tatcgtcagg cggtgaatga aaataccgca ctgttgatga aagtacatac cagtaactac
     2281 agcattcagg ggttcaccaa agcgatagat gaagcggaac tggtggcgct cggcaaagag
     2341 ctggatgttc ccgtagtgac tgatttaggc agtggctcgc tggtcgatct tagccagtac
     2401 ggtttgccga aagagccaat gccgcaggag ttgattgcgg cgggcgtcag tctggtgagc
     2461 ttctccggcg acaagttgtt aggcgggccg caggcaggga ttatcgttgg taaaaaagag
     2521 atgatcgccc gcctgcaaag ccacccgctg aagcgtgcat tacgcgcgga taaaatgacc
     2581 ctcgcggcgc tggaagccac gttgcgtctt tatttacacc ctgaagctct gagtgaaaaa
     2641 ttaccgaccc tgcgcctgct tacccgcagc gcagaggtca ttcaaatcca ggcacaacgt
     2701 ttacaggctc cccttgccgc acattacggc gcggagtttg cggtacaggt tatgccatgt
     2761 ctttcgcaga ttggcagtgg ttcgctgccg gttgatcgcc tgccgagcgc ggcattaacg
     2821 tttacacccc atgatggacg cggtagccac cttgagtcat tagccgcccg ctggcgtgaa
     2881 ttgccagtgc cggtgattgg tcgtatttat gacggacgat tgtggctgga tttacgctgc
     2941 cttgaagatg agcaacggtt tttggagatg ttgttgaaat gattattgcg actgccggac
     3001 acgttgacca cggcaaaaca accttattgc aggcgattac tggcgtaaat gctgaccgtc
     3061 tgccggaaga aaaaaagcgc ggcatgacca tcgatctcgg ctatgcctac tggccgcagc
     3121 cggatggtcg cgtgcctagt tttatcgacg tacccggtca tgaaaagttt ctttccaaca
     3181 tgctggcggg cgttggtggt atcgatcacg cgctgttggt ggtggcgtgc gatgacggcg
     3241 tgatggcgca gacccgtgag catctggcga ttttgcagct gaccggtaac ccgatgctga
     3301 cagtggcgct gaccaaggcc gatcgcgtgg acgaagcgcg tgttgatgag gttgaacgcc
     3361 aggtaaagga ggtgctgcgg gaatacggtt ttgctgaggc aaaactgttt atcaccgcag
     3421 caaccgaagg tcggggaatt gatgccctgc gcgagcatct gcttcagtta ccaggacgtg
     3481 aacacgccag ccaacatagt ttccgcctcg cgatcgaccg cgcatttacc gtaaaaggtg
     3541 ccgggctggt cgtcaccggt acggcgttaa gcggggaagt gaaggtaggc gattcactct
     3601 ggctgactgg tgtaaataaa ccgatgcgtg tacgtgcgct gcatgcacaa aaccagccaa
     3661 cagaaaccgc ccatgccggg cagcgtatcg cgcttaacat cgcgggtgat gcggaaaaag
     3721 agcagattaa ccgtggcgac tggctgcttg ccgatgcgcc gccagagccg ttcacacggg
     3781 tgattgtcga gcttcaaacc catacaccgc tgacccagtg gcagccgctg catattcacc
     3841 acgccgccag ccacgtcacg ggacgcgttt cactgctgga agataacctt gctgaactgg
     3901 tcttcgacac cccgttatgg ctggcagata acgaccgcct ggtattgcgc gatatctctg
     3961 cccgcaacac gctggccgga gcgcgcgtcg tgatgcttaa cccgccgcgt cgcggtaaac
     4021 gtaaaccaga gtatctgcaa tggctggctt ccctcgccag ggcacagaat gatagcgaag
     4081 cgttagccgt tcacctgcaa cgcggcgcgg ttaatcttcc tgatttcgcc tgggcgcgcc
     4141 agctcaacgg cgaagggatg cgcgaactgc tgcaacagcc tggctatatt caggctggtt
     4201 atagcttgtt gaatgcgccg gttgccgctc gctggcaacg gaaaattctc gacacattag
     4261 cgacttatca tgagcaacat cgcgatgaac ctgggcctgg gcgcgaacgt ctgcgacgta
     4321 tggcgttgcc aatggaagat gaagcgctag tactgttgct gattgaaaag atgcgcgaaa
     4381 gcggcgacat cctcagtcat cacggttggc tgcatctgcc ggatcacaaa gcgggcttca
     4441 gcgaagagca gcaggccatc tggcaaaaag cagagccgct gtttagtgac gagccgtggt
     4501 gggtgcgtga tctggcaaaa gagacgggta ccgacgagca ggcaatgcgc ttgacgctaa
     4561 gccaggcggc gcagcaagga ataattaccg cgatcgtcaa agatcgttat taccgtaacg
     4621 atcggattgt cgaatttgcc aatatgatcc gcgatctcga tcaggagtgt ggttcaacct
     4681 gcgcggcgga tttccgcgat cgcttaggcg taggccgaaa gctggcaatt cagattctgg
     4741 aatattttga ccgcattggc tttacgcgtc gtcgtggaaa tgatcattta ttgcgcgacg
     4801 cattattatt tccggaaaaa taaggaaatg attaattaat ttttaaaata aattaataca
     4861 aatttcttat gattttaaaa aaagcacatt gtttaatgaa tacaatgtgc tttttattag
     4921 attaattttg ataattaata ataatgatca tgttttcgaa tgaaaatcct cagtaagctt
     4981 cccacccttt tttacacttt caggagtgtg ttatggcatc ttcaactttc tttattcctt
     5041 ctgtgaatgt catcggcgct gattcattga ctgatgcaat gaatatgatg gcagattatg
     5101 gatttacccg taccttaatt gtcactgaca gtatgttaac gaaattaggt atggcgggcg
     5161 atgtgcaaaa agcactggaa gaacgcaata tttttagcgt tatttatgat ggcacccaac
     5221 ctaaccccac cacggaaaac gtcgccgcag gtttgaaatt acttaaagag aataattgcg
     5281 atagcgtgat ctccttaggc ggtggttctc cacacgactg cgcaaaaggt attgcgctgg
     5341 tggcagccaa tggcggcgat attcgcgatt acgaaggcgt tgaccgctct gcaaaaccgc
     5401 agctgccgat gatcgccatc aacaccactg cgggtacggc ctctgaaatg acccgtttct
     5461 gcatcatcac tgacgaagcg cgtcatatca aaatggcgat tgttgataaa catgtcactc
     5521 cgctgctttc tgtcaatgac tcctctctga tgattggtat gccgaagtca ctgaccgccg
     5581 caacgggtat ggatgcctta acgcacgcta tcgaagcata tgtttctatt gccgccacgc
     5641 cgatcactga cgcttgtgca ctgaaagccg tgaccatgat tgccgaaaac ctgccgttag
     5701 ccgttgaaga tggcagtaat gcgaaagcgc gtgaagcaat ggcttatgcc cagttccttg
     5761 ccggtatggc gttcaataat gcttctctgg gttatgttca tgcgatggcg taccagctgg
     5821 gcggtttcta caacctgcca cacggtgtat gtaacgccgt tctgctgccc catgttcagg
     5881 tattcaacag caaagtcgcc gctgcacgtc tgcgtgactg tgccgctgca atgggcgtga
     5941 acgtgacagg taaaaacgac gcggaaggtg ctgaagcctg cattaacgcc atccgtgaac
     6001 tggcgaagaa agtggatatc ccggcaggcc tacgcgacct gaacgtgaaa gaagaagatt
     6061 tcgcggttct ggcgactaat gccctgaaag atgcctgtgg ttttactaac ccgatccagg
     6121 caactcacga agaaattgtg gcgatttatc gcgcagcgat gtaatcatca tttccacaac
     6181 ggctggcaaa ttgtcagccg ctttttcaac tatctctgta acccttgccc gtaaattcgt
     6241 gatagccgtc gtaaagctgt taccgactgg cgaagatttc gccagtcacg tctacccttg
     6301 ttatacctca caccgcaagg agacgatcat gaccaataat cccccttcag cacagattaa
     6361 gcccggcgag tatggtttcc ccctcaagtt aaaagcccgc tatgacaact ttattggcgg
     6421 cgaatgggta gcccctgccg atggtgagta ttaccagaac ctgacgccgg tgactgggca
     6481 gctgctgtgc gaagtggcgt cttcgggcaa acgagacatc gatctggcgc tagatgctgc
     6541 gcacaaagtg aaagataaat gggcgcacac ctcggtgcag gatcgcgcgg cgattctgtt
     6601 taagattgcc gatcgaatgg aacaaaacct cgagctgtta gcgacagctg aaacctggga
     6661 taacggcaaa cccattcgcg aaaccagtgc tgccgacgtg ccgctggcga ttgatcattt
     6721 tcgctatttc gcctcgtgta ttcgggcgca ggaaggtggg atcagtgaag ttgatagcga
     6781 aaccgtggcc tatcatttcc atgaaccgtt aggcgtggtg gggcagatta tcccgtggaa
     6841 cttcccgctg ctgatggcga gctggaaaat ggctcccgcg ctggcggcgg gcaactgtgt
     6901 ggtgctgaaa cccgcacgtc ttaccccgct ttctgtactg ctgctaatgg aaattgtcgg
     6961 tgatttactg ccgccgggcg tggtgaacgt ggtcaatggc gcaggtgggg aaattggcga
     7021 atatctggcg acctcgaaac gcatcgccaa agtagcgttt accggctcaa cggaagtggg
     7081 ccaacaaatt atgcaatacg caacgcaaaa cattattccg gtgacgctgg agttgggcgg
     7141 taagtcgcca aatatcttct ttgctgatgt gatggatgaa gaagatgcct ttttcgataa
     7201 agcgctggaa ggctttgcac tgtttgcctt taaccagggc gaagtttgca cctgtccgag
     7261 tcgtgcttta gtgcaggaat ctatctacga acgctttatg gaacgcgcca tccgccgtgt
     7321 cgaaagcatt cgtagcggta acccgctcga cagcgtgacg caaatgggcg cgcaggtttc
     7381 tcacgggcaa ctggaaacca tcctcaacta cattgatatc ggtaaaaaag agggcgctga
     7441 cgtgctcaca ggcgggcggc gcaagctgct ggaaggtgaa ctgaaagacg gctactacct
     7501 cgaaccgacg attctgtttg gtcagaacaa tatgcgggtg ttccaggagg agatttttgg
     7561 cccggtgctg gcggtgacca ccttcaaaac gatggaagaa gcgctggagc tggcgaacga
     7621 tacgcaatat ggcctgggcg cgggcgtctg gagccgcaac ggtaatctgg cctataagat
     7681 ggggcgcggc atacaggctg ggcgcgtgtg gaccaactgt tatcacgctt acccggcaca
     7741 tgcggcgttt ggtggctaca aacaatcagg tatcggtcgc gaaacccaca agatgatgct
     7801 ggagcattac cagcaaacca agtgcctgct ggtgagctac tcggataaac cgttggggct
     7861 gttctgatat aagaagctgg tcgcattggg tattcattgc ctgatgtgac gcttacgcgt
     7921 cttatcatgc ctacgggaac ctgaccgtag gccggataag gcgtacacgc cgcatccggc
     7981 ataaacaaag cgcacgttgt tacaatttac gttttaaacc tacgtgcgca ataatccaga
     8041 ctacgccaac agaagtgcgt ttctggggcg tgttttgtca ggagtttaat ctcgcgtttc
     8101 gatggtgagc gggcgaacta ggccgtaaat caccattggt gatccgaggc caatcagcaa
     8161 taaacg
//
LOCUS       DAJTOR010000178         8162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTOR010000178 DAJTOR010000000
VERSION     DAJTOR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.178"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(88..624)
                     /gene="rcdA"
                     /locus_tag="OO249_003527"
     CDS             complement(88..624)
                     /gene="rcdA"
                     /locus_tag="OO249_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5637687.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            708..1916
                     /locus_tag="OO249_003528"
     CDS             708..1916
                     /locus_tag="OO249_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5637688.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            1916..2731
                     /gene="ybjI"
                     /locus_tag="OO249_003529"
     CDS             1916..2731
                     /gene="ybjI"
                     /locus_tag="OO249_003529"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5637689.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            2817..3101
                     /locus_tag="OO249_003530"
     CDS             2817..3101
                     /locus_tag="OO249_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637690.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(3142..4372)
                     /gene="mdfA"
                     /locus_tag="OO249_003531"
                     /pseudo
     CDS             complement(3142..4372)
                     /gene="mdfA"
                     /locus_tag="OO249_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            4657..5253
                     /gene="ybjG"
                     /locus_tag="OO249_003532"
     CDS             4657..5253
                     /gene="ybjG"
                     /locus_tag="OO249_003532"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5637691.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5311..6069
                     /gene="deoR"
                     /locus_tag="OO249_003533"
     CDS             5311..6069
                     /gene="deoR"
                     /locus_tag="OO249_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5637692.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6116..7318)
                     /gene="dacC"
                     /locus_tag="OO249_003534"
     CDS             complement(6116..7318)
                     /gene="dacC"
                     /locus_tag="OO249_003534"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5637693.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKDVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            7565..8162
                     /gene="gstB"
                     /locus_tag="OO249_003535"
                     /pseudo
     CDS             7565..8162
                     /gene="gstB"
                     /locus_tag="OO249_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
BASE COUNT         1969 a         2085 c         2021 g         2087 t
ORIGIN      
        1 caacctaact tattgttttt aatctaactt attgttttca ttaacccgtt tacataaatg
       61 ggttttttgt tatctaaaat ttatctacta ccctgcaacc ctctcaacca tcctcaaaat
      121 ctcctcgcgc gatagcggct tacggtcggt gacaaaatgc agcgtcatcc cctcaataaa
      181 cgcatcaagc gcgcgggcgg ttccgggttc aaaccattgt tcgagcgttt gctgactgcg
      241 ctgcatccag ttttgcatta ccgtttttaa tagcggtttt cggctagcca gcgcgtagag
      301 ctggtacatc agctccatat tatccggcgt tgcaacctgt gagctgtaga tcatatcggt
      361 gatagcctgg catgcgcctt gagcatcact aacatcgcta aaaaatgcct gatattgccg
      421 ggacataatc tcagtaaaac tgctgaacgc ctccagcaac aactcatcaa ttcctgaaaa
      481 atagtaggtc atcgatccca acggtacccc ggcaagggta gcaattttgc ggtgcgtaac
      541 agcatgtatt ccgtaaagtt tcaccgcctc cagcgtggcc tggataattt tttctcgccg
      601 ttgcggatcg ttagcgcgac gcatgtcatt tcctctgtct gcaattgtgt acaaatgtac
      661 acaaccttgc taatgttgtc ttccctcttc ttattttgac gtgatctatg accgtaaatt
      721 cttcacgtaa tgcattgaaa cgccgaacct gggcgctgtt tatgttcttc tttttgccag
      781 gcctgttaat ggcgtcctgg gcaacccgta cgcctgctat ccgcgatatt ctctctgtct
      841 cgatcgctga aatgggcggt gttctctttg gtctgtcgat cggttcgatg agcggtattc
      901 tctgctcggc gtggttagtg aaacgctttg ggacacgtaa tgtcatcctg gtcacgatgt
      961 cctgcgcatt gatcgggatg atgatattaa gtctggcact ctggctgaca tcgcccctgc
     1021 tctttgccgt tggtctcggc gtctttgggg caagttttgg ttctgcggaa gtggcgataa
     1081 acgttgaagg tgccgccgtt gagcgagaaa tgaataaaac ggttttgccg atgatgcacg
     1141 gtttttatag cctgggcacg ctggcaggcg ctggtgtcgg gatggcactg acggcctttg
     1201 gcgttccggc aacggtgcac attttattgg cggcgctggt aggtatcgcg cctatttata
     1261 tcgccattca ggcaatccct gacggtacgg gcaaaaatgc tgccgatggc acccagcatg
     1321 gcgaaaaagg cattcctttt tatcgcgata tccagttgct gttgattggt gttgtagtgc
     1381 tggcgatggc ctttgccgaa ggttctgcca acgactggtt acccttatta atggttgacg
     1441 gtcacggttt tagccctact tccggctcgc tgatttatgc cggttttacc ctggggatga
     1501 ccgttggacg cttcactggc ggttggttca tcgaccgtta cagtcgcgtt gccgtggttc
     1561 gggccagtgc actaatgggg gcgttgggta ttgggctgat tatttttgtc gatagcgcct
     1621 gggtcgctgg ggtgtctgtt gtactctggg gactgggtgc ctcgctgggc ttcccgctga
     1681 ccatttctgc cgccagcgat accggccccg atgcaccaac ccgcgtcagt gtggtagcaa
     1741 cgaccggtta tctggctttc ctcgtcgggc cgccgctgct gggctatctc ggcgaacatt
     1801 atggattacg tagtgcaatg ctggttgtac tggcgctggt tattctcgcg gctattgtcg
     1861 cgaaagccgt cgccaaaccc gataccaaaa cgcagacggc gatggagaat agttgatgag
     1921 cattaaatta attgcggtag acatggatgg tactttctta agcgatcaaa aaacctataa
     1981 ccgtgagcgg tttatggctc agtatcagca aatgaaagca caaggcattc gctttgtggt
     2041 cgccagcggg aatcaatatt atcagttgat ctctttcttc cctgaaattg ctaatgaaat
     2101 tgcctttgtg gctgaaaacg gcggctgggt agtgagtgaa ggcaaagatg tttttaatgg
     2161 cgagctgtcg aaggatgcct ttactactgt cgtggaacat ttgctgacgc gcccggaagt
     2221 ggaaattatt gcctgcggaa aaaatagcgc ctatacgctc aaaaaatatg acgatgccat
     2281 gaaaacggtg gcggaaatgt attatcaccg tctggaatac gttgataact ttgacaactt
     2341 agaagatatt ttctttaagt ttggcctgaa tctttccgat gaactgattc cacaagtaca
     2401 aaaagcatta catgaggcca tcggcgatat tatggtgcca gtccacaccg gcaacggcag
     2461 catcgatctg attatccccg gcgtacataa agccaatggc cttcgccaac tgcagaaatt
     2521 atggggaata gacgacagcg aagtggtggt ctttggcgat ggcggtaacg atattgagat
     2581 gctgcgtcag gcaggcttta gttttgcaat ggaaaatgcc ggcagcgcgg tcgtcgcagc
     2641 ggcaaaatac cgggcaggct ccaataaccg tgaaggcgta ctggatgtga tcgataaagt
     2701 gcttaaacac gaagcgccat ttgaccaata aaaacggcag cacgcccggc aacacaatac
     2761 ctgaatgaag gctgcataat tcgcggaaat taacccgctg cattatttca ggcgaaatga
     2821 ttatgaaaaa ttgtctactg ttgggcgcgc ttttaatggg ctttactggt gtggcgatgg
     2881 cgcaaagtgt caccgtcgat gtgccgagtg gatacaaagt ggttgtagtt cctgattcag
     2941 tcagcgttcc acaagcggtc agcgtcgcga ctgtgccgca aaccgtctat gtggctcctg
     3001 cgccagctcc ggcttaccgc ccgcatcctt atgctcgcca tcttgccagc gtaggtgaag
     3061 ggatggttat cgaacaccag attgacgacc atcaccatta attcgagaat gcctgatcgc
     3121 acaaagcagt caggcatttt tttacccttc gtgagaattt cccatctgtt tatcttttaa
     3181 aaagataacc atcagcgaca gccacaaaat tccgttgaca aggttgaaga gattaaactg
     3241 tccgttgccc ccgttcagcc aggcatgttt gctgatttca ataccaacgg taaagatcag
     3301 catttgcagc attcccatcg ccgcagaaac cgtaccttta ctcatatcgc tggcaaacag
     3361 ggttaatcgc accagtcccg cattcgccag accaataccg aaagcataaa tacttaaccc
     3421 ggcagtcatc cataaatacg cgtgcgatga gataaccgtt gccgcagcag cgaccaacag
     3481 accaatcata atcggccagc cgcccataat tatcagcgaa cgtacggtgc ggcacgaggt
     3541 cagacgcgct aacagcaagt tacccgcaat taacgccccg aaaataggca cttgcagcaa
     3601 gccatattca tagctgctca actgctcgcc ggtaatgatg ataatcggcg actgggcgat
     3661 ccacgccagc aatggcaggc taacgaatcc cagcgccagc gcccccgcca caaagcggcc
     3721 gttcttcagc accagcttat agtcacgacc gagttctttc agtgacagtt tctcgcctat
     3781 acgcgtggcg gtttcaggca tagctcgttg cagaccgaaa aaggagatcg ctgccaatgc
     3841 ggcaaacaaa acaaacatcc cctcccaggg cagcacatgg atccacgccg cgcccaccag
     3901 cggaccaagt agcggagcaa tcagcgccac gttcgccatc agcgcggtga tcttgataca
     3961 aaccgcctct tcgaaagatt cctgaattgc ggcgtatccc acagcgccaa tgaaacagag
     4021 gcttatgccc tgcaagaagc gcaacagagt gaattgttca atgttttgcg ccagtaatat
     4081 tgccagacag gtgatgataa accacaccac ccccgccagc atcaccggac ggcgaccaat
     4141 acgatccgac agcggcccca ggagccattg taaaaacatc ccgcccgcca gatatgcggt
     4201 cattgaagta ggaacccaat caatgcccgc ctgatattgt tccaccacgg ccaacatacc
     4261 gggttgaatc atatctgccg atataggttg aaaattcgta aagcaccaga cagagaggga
     4321 aaagtaacgc ctgacgtcca agcctggcac cggaagctaa tttattttgc atgcaatttc
     4381 ttcgccaata ataatcgcgc agagtttaat aaaagcgcag ctaacgagaa agcgaatttt
     4441 gtagctgaaa ccacggttaa gcacattctt acattattgc gagtatagct acgctttctt
     4501 taagttttat ttaacctatg cccgttacaa tcacccaccg taaacaggcc gcttgaggga
     4561 aataagacga tgccgcttta cccagtttaa cctgcacttt attctcaacg acttgcctgt
     4621 attggctccc ttttaatcac tttgcgtcgg gaagttatgc tggaaaattt gaatctctct
     4681 ctattctctc ttattaacgc gacgccagac tcggctccgt ggatgatctc gttggcgatt
     4741 tttattgcta aagatttgat taccgtggtg ccgctgctgg ccgcggtgct ttggttgtgg
     4801 gggcttacag cacaacggca actggtgata aaaatcgcta tcgcgctggc ggtcagcctg
     4861 tttgtgtcct ggacgatggg acatcttttt ccgcacgacc gaccctttgt cgaaaatatc
     4921 ggctataact tcctgcatca tgcggcggat gactcattcc caagcgacca cggtacggtg
     4981 attttcacct ttgcactggc atttttatgc tggcatcgcc tgtggtccgg ctcactttta
     5041 atggtgctgg ccgtcgtcat tgcctggtcg cgcgtttatc ttggcgtcca ctggccgctg
     5101 gatatgctcg gtggattgct ggcaggtatg attggctgcc ttagtgccca gattatctgg
     5161 caagcgatgg ggcataaact ctatcaacgt ctgcaatcgt ggtatcgctt ctgttttgca
     5221 ttaccgatcc gcaaaggctg ggtgcgtgac tgatctctgc taaaaagtgt agtattgagc
     5281 ggctcgcttc aataactatt cagagggatt atggaaacac gtcgcgaaga gcgtatcggg
     5341 cagctgctgc aagaattaaa acgcagcgat aagttacatc ttaaagacgc cgccgccctg
     5401 cttggggttt cggagatgac gattcgtcgc gatctgaaca accacagtgc gcccgtcgtt
     5461 ttgctcggcg gctatattgt tctggaaccg catagtgcca gccattacct gttaagcgat
     5521 caaaaatccc gcctggtgga agaaaaacgc cgggcggcaa aactggctgc gacactggta
     5581 gaacccgatc agaccctctt ttttgactgt ggcaccacca cgccgtggat tattgaagcg
     5641 attgataatg aaatcccttt taccgccgtt tgttattcgc taaatacctt tctggcgctg
     5701 aaagagaaac cccattgccg cgcatttctg tgcggtggtg aatttcacgc cagcaacgcc
     5761 attttcaaac ccatcgattt tcagcaaacg ttgaataatt tttgcccgga tatcgctttt
     5821 tattccgcgg cgggcgtgca tgtcagtaaa ggcgctacct gttttaatct tgaagagttg
     5881 ccggtaaaac actgggccat gtcgatggcg caaaagcatg tgctggttgt cgaccacagt
     5941 aaatttggca aggtccgtcc ggcgcgcatg ggtgacctga aacgctttga tattgtggtg
     6001 agtgattgtt gcccggaaga tgagtatgtg aagtacgcgc agacgcaccg tattaagttg
     6061 atgtattaat gacgtataac cggatgacgt ttcgcgccat ccggttatca gaagattaag
     6121 agaaccagct gccgaaccac tgatggaatt tcatcatcac gaaatcccac atccgaccaa
     6181 agaatccgcc ctcttccaca ttttccatca cgatcagcgg acgctgctca atggatttac
     6241 cgttaagctg gaaatcaatg gtcccgacaa cctgaccttt tttcagcggt gcggtaagct
     6301 gcggttccgt taacgtgtag ctcgccttca ggtttttcag ctgcccacgc ggtatggtca
     6361 ctgagcccgc ttcgcctgcc ccgagattca cttcgctctt atcaccaaac cagacgcgct
     6421 gagtcacaaa ggtggcatca ggtttaattg gcgtcacggt ttcaaagaag cggaaacccc
     6481 aggtcaataa tttctcagac tcattaaaac ggatacggtc agttttcgcc cccaacacta
     6541 cggagattaa acgcatatcg ccctgggtag ccgaagcaac cagattatat cccgcgcctg
     6601 ccgtggttcc tgtcttcatg ccatcaacat tcagattgct gctccacagc agacggttac
     6661 ggttaggctg acgaattttg ttgaaggtga actctttctc tttatgaatg gcgtactctt
     6721 ccggcacatc gtggatcaat gctttaccca gcaatgccat atcgcgcgcg gtactgaact
     6781 gccccggcgc atccaggccg tgcaccgtct ggaaggtagt gttggtcaga cccagttttt
     6841 tggcataacc attcatcaga ccaataaatg actcctggct cccggcaacg taatcggcca
     6901 gcgcaataca ggcgtcatta ccggactgga taatcacatc tttgttcaag tctgccaccg
     6961 aaacctgatc gcccggtttg aggaacatta ccgatgaacc acgcagtgcc ggatttcccg
     7021 tcgcccaggc atctttaccg accgtcacca tatcggtgag tttaatctta tcggccttaa
     7081 gcgcctgccc aaccacatag ctggtcatga ttttagtcag gctcgcggga tccagtttct
     7141 catccgcatt gccttctgcc agcactttac cgctggcgta atccattaaa atccatgcac
     7201 gcgcatccac gctcggcgct tcaacggttt gttcggccgc gaatgccgtt ggggcaaaaa
     7261 ggaataaaaa tgcagaaccc gctgcaagac cacgaaggag agaggagtat tgcgtcataa
     7321 atgccacccg agtatccatt caaaaaaaat acgttatatc gccgtatgat aagttcggtg
     7381 attaataacg cacacacaaa cttaaagaaa ccgtaagttg gtaaagtttt taaagtttat
     7441 gcaatatcct gccgcttcgc tgcttctggt gtcatttttt tgccagctgt tgcttaaaag
     7501 ttaactttac ttcaccatcg gagtcctgtt cagcctgtgg ctgcctgtcg gaaaaggggt
     7561 gagtatgatt acgctgtggg gtcggaataa ttcaaccaac gtaaaaaagt attgctgacg
     7621 ctcgaagaac tggaactacc ttatgagcaa attctcgcgg gccgtgagtt tgggataaat
     7681 cacgatgctg attttctggc gatgaaccct aacgggctgg tgccgttgtt gcgtgacgac
     7741 gaaagtgatc tcattctttg ggaatcaaac gccattgtcc gctatctggc ggcacagtac
     7801 gggcaaaaac gcctgtggat cgactcaccg gcacgtcgtg cggaagcgga aaaatggatg
     7861 gactgggcaa accagacgct cagtaatgcc catcgaggga tcctgatggg attagtcaga
     7921 acaccaccgg aagagcgcga tcaggccgcc attgatgcca gttgcaaaga gtgcgacgcc
     7981 ctgtttgccc tgctcgatgc ggaactggca aaagtaaaat ggttctctgg cgacgagttt
     8041 ggtgtgggcg atatcgctat cgcaccgttc atctacaatt tgtttaacgt tggcctgacc
     8101 tggacaccgc gtccaaatct gcaacgctgg tatcagcaac tcactgaacg ccccgcagtg
     8161 cg
//
LOCUS       DAJTOR010000179         8147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTOR010000179 DAJTOR010000000
VERSION     DAJTOR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.179"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..260
                     /locus_tag="OO249_003536"
     CDS             <1..260
                     /locus_tag="OO249_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5637694.1"
                     /translation="VVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRF
                     YRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(399..1028)
                     /locus_tag="OO249_003537"
     CDS             complement(399..1028)
                     /locus_tag="OO249_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5637695.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(1151..2797)
                     /gene="fumB"
                     /locus_tag="OO249_003538"
     CDS             complement(1151..2797)
                     /gene="fumB"
                     /locus_tag="OO249_003538"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5637696.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGIAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDTCHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2875..4215)
                     /gene="dcuB"
                     /locus_tag="OO249_003539"
     CDS             complement(2875..4215)
                     /gene="dcuB"
                     /locus_tag="OO249_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5637697.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4786..5505)
                     /gene="dcuR"
                     /locus_tag="OO249_003540"
     CDS             complement(4786..5505)
                     /gene="dcuR"
                     /locus_tag="OO249_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5637698.1"
                     /translation="MSNVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5502..7133)
                     /gene="dcuS"
                     /locus_tag="OO249_003541"
     CDS             complement(5502..7133)
                     /gene="dcuS"
                     /locus_tag="OO249_003541"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5637699.1"
                     /translation="MRHSLPYRMLLKRPMKLSTTVILMVSAVQFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVVSGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYSLKTANNYKEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            7314..7544
                     /locus_tag="OO249_003542"
     CDS             7314..7544
                     /locus_tag="OO249_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCR5637700.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            7556..7828
                     /locus_tag="OO249_003543"
     CDS             7556..7828
                     /locus_tag="OO249_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5637701.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            8055..>8147
                     /gene="ghoS"
                     /locus_tag="OO249_003544"
     CDS             8055..>8147
                     /gene="ghoS"
                     /locus_tag="OO249_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5637702.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLM"
BASE COUNT         2023 a         1969 c         2084 g         2071 t
ORIGIN      
        1 cggttgtgtt agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat
       61 taggtatgca gtttattatg attgctctac caactctgtt tttcatggta acgctgattc
      121 tctacttccg tttctatcgc ctcaatggtg acacgctgcg caggatccag atccatctgc
      181 tggataaata tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag
      241 tgagtgatgt gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt
      301 ttatcaggcc acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg
      361 aattcacgct gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag
      421 cgccgtagct ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc
      481 agcgcgcaga acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca
      541 tccgctccct gacccattgc cagcgccgcc caccactcga ttaattcata cattgcgctt
      601 atcgccagcg ccacgcagca gactaggaac gccaccattt tacgtccgcg cacgtacatc
      661 ccgcgaacga gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaagtgcccc
      721 agcttgtcat acggattacg gctcaacccc agccattcct gcacctcaaa accaacgggg
      781 actttcgcgt aggtgtattg tccgccgacc atcaggatga tggcgtgaag gaaaatgagc
      841 gtatagagca gcggcgttaa cggataacgt ctggcggtgg caagcagtag cggcacgaca
      901 ataatcaccg gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta
      961 taaatcagga ttaatgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg
     1021 cgggtcatgg tgagttcgca aatcagggaa aatactgact attcaccagc aagcgtgaaa
     1081 ttacaatcgc aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg
     1141 gccgaagagg ttacttagtg cagttcgcgc actgtttgtt gacgatttgc tggaagaagt
     1201 cgttaccttt gtcatcgacc aagataaacg ccgggaaatc ttctacttcg attttccaga
     1261 tagcttccat acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg
     1321 ccagtaccgc cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg
     1381 tgtcggtaac ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg
     1441 attgcagcag atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg
     1501 atggataacc ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt
     1561 actgcggaag ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc
     1621 ggcccacgat aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct
     1681 gggcgaggat ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg
     1741 cctggcgcag ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga
     1801 taccttcgcg gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc
     1861 cgaccgggca ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt
     1921 atttaccgcc aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt
     1981 ccagctggac atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt
     2041 aatagtgagc gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa
     2101 tcacaaacgc gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga
     2161 cgaggaagtt tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg
     2221 ttttgttggc agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta
     2281 ccgcgtacag gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt
     2341 ccagcgccgc attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt
     2401 tcgacagcgc ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga
     2461 tgatcgcgat gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggaat
     2521 ttcttaagaa ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa
     2581 tagccgcaac ctgtttctgg tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt
     2641 gctgcgccag cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt
     2701 cggcaacgct aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg
     2761 ggaaaggtgc ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta
     2821 tttgttattc gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa
     2881 gaacccgtac atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa
     2941 ccccggcaga ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg
     3001 gtcaaactga atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca
     3061 agccggtgct gaagccacga tgtatgccgg atcaacgccg atcgccaacg cgaccggaac
     3121 aatcgccgcc agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat
     3181 ggcataggcc cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat
     3241 atgcgcaccg aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat
     3301 cataccggaa cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat
     3361 aataatcagc gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg
     3421 tttgccgccg aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat
     3481 tgccccgagg aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatccagcag
     3541 cgtcgcggta tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa
     3601 ctcttcgtct ttatccagat ctttaccacg gaaccagctg aagataccga tcgccaggat
     3661 accgattaac gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc
     3721 atcaaaggtg acattaccca gcatcgcaac cagagacacg accgcaaccg acaccggact
     3781 ggcgataatc cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat
     3841 gttgttctta atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt
     3901 accgcaaaga atggtcagcg tacaggtcac aaacggcgcg acaattgaga catatttcgg
     3961 gttgcggcgc agcaacttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg
     4021 caaggtcgcc gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt
     4081 acctggctga aggtggaaga caaagaccag aatgaccaga ccgataccgc ctaataaacc
     4141 cagcgcgata cccccctttc tggcaccata aaacagacat atcagtatta tgataagttg
     4201 gatagtaaat aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga
     4261 tcacagtcac gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg
     4321 tcgttaccgg ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa
     4381 tttatgcact gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata
     4441 gtttgtactg cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc
     4501 tattttttgt tctcagggca gatatcatta ggtttaaaga ttaatcttat ctacccattt
     4561 gtgggtgaaa atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt
     4621 tgaaattaaa aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata
     4681 attatatttg atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt
     4741 ttgcattacg gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg
     4801 tttcagtagt gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac
     4861 gccataatgg atactggtga acaagatgtg gcagttgacc agccagatga ggtatttacg
     4921 gcaggaaaca cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc
     4981 ctgatgcgcg tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg
     5041 caagcgacga ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc
     5101 ctggtcataa tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc
     5161 ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag
     5221 cgaatcttta atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca
     5281 acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc
     5341 gagcaatatc aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag
     5401 cgtcgaggct gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag
     5461 ctccgcgacc attgcgtcgt catcgataat taatacattg ctcatctgtt tgacctctcc
     5521 ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg
     5581 atgctgccgc cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg
     5641 cttccttttg tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc
     5701 ggtccatcat cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta
     5761 atttcgccac cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat
     5821 gtggtaatca gcgtcgcgac ctggtcctca ctgccgctgt ctggcagcag gctttcactg
     5881 tttaaaatca gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca
     5941 gcgataaccg gagatttgat cttacccagc agagagccaa tctcttcctt atagttattg
     6001 gctgttttga gactgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga
     6061 atcacatgca atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg
     6121 accagaccgt cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa
     6181 atggcaccga tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg
     6241 tctttaatcg taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca
     6301 tctaccacct gtgaccatga gtggcttagc gtcgacagtt tctcatcgtc ctgcgactta
     6361 cggtaattca gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc
     6421 acggcaacga cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg
     6481 gagatttcgt agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag
     6541 gtgccaatca gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg
     6601 tcattgattt gttgggtcac gcggcttaac tcaaggccgc tcaccaccac gccaatttgt
     6661 ttatgatttt catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga
     6721 ttgatagcga cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc
     6781 tgaccaatac gctgggcttc aggatgcgag tagcgaagac tttgcatatc cgtaacgaca
     6841 ataaacagca gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc
     6901 tgcggttttt tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact
     6961 gccagtgcct tgttggccag cccgtctcgt gtcatatcac tgatttgcga gaagtaaatc
     7021 agatgcacca ccaatagcac cgagaactgt accgcgctga ccattaagat cactgtggta
     7081 ctcaatttca tcggacgttt gagtaacatg cggtagggca atgaatgtct catcagcttc
     7141 cttgtgtgac aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa
     7201 gaatgattgg ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa
     7261 gattccaggg ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc
     7321 aggcgctact ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca
     7381 acactgcgat atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc
     7441 tcaaacgaaa gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga
     7501 ttgatacgtg cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga
     7561 aatacgcgaa ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga
     7621 aattgtcttt gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga
     7681 aagcctgaaa gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg
     7741 tcgggaaaaa cgaaaaatta tcccactatg cccatttgcg aaacacgaat ttgataaaac
     7801 gcgggagtat gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg
     7861 ctctccgcgg agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc
     7921 agagagcgcg tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc
     7981 ctatatgaga atcatcaatc ggggttaata agttttgcgt ccccagagca ttcaatattg
     8041 ataggagtca tattatggaa ggtaaaaaca agttcaatac ttatgttgtt tcttttgatt
     8101 atccatcatc ttattcctca gtgttcttaa ggttaagatc attgatg
//
LOCUS       DAJTOR010000180         8069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTOR010000180 DAJTOR010000000
VERSION     DAJTOR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.180"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..257
                     /locus_tag="OO249_003545"
     CDS             <1..257
                     /locus_tag="OO249_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5637703.1"
                     /translation="AIAGSTAQGGVIHFYGQIVEPACDVSTQSSPVEMNCPQNGSVPG
                     KTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEYN"
     gene            353..886
                     /gene="ymdB"
                     /locus_tag="OO249_003546"
     CDS             353..886
                     /gene="ymdB"
                     /locus_tag="OO249_003546"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5637704.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            828..2309
                     /gene="clsC"
                     /locus_tag="OO249_003547"
     CDS             828..2309
                     /gene="clsC"
                     /locus_tag="OO249_003547"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5637705.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLVAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2317..3474)
                     /gene="mdoC"
                     /locus_tag="OO249_003548"
     CDS             complement(2317..3474)
                     /gene="mdoC"
                     /locus_tag="OO249_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5637706.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3868..5403
                     /gene="mdoG"
                     /locus_tag="OO249_003549"
     CDS             3868..5403
                     /gene="mdoG"
                     /locus_tag="OO249_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5637707.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGGAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5396..7939
                     /gene="mdoH"
                     /locus_tag="OO249_003550"
     CDS             5396..7939
                     /gene="mdoH"
                     /locus_tag="OO249_003550"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5637708.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKHWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2021 a         1924 c         2129 g         1995 t
ORIGIN      
        1 ttgccatcgc aggaagcacc gcgcaagggg gcgtgatcca tttttatggc caaattgtgg
       61 aaccggcatg tgacgtcagc acccagtcat cacccgtaga aatgaactgc ccacagaatg
      121 gttctgttcc gggaaaaacc tattccagca aagcgttaat gagcggcaat gtcaaaaacg
      181 cgcaagtagc ctcagtaaaa gtgcagtata ttgataaaca gaaaaagctg gctgtaatga
      241 acatcgaata taactaagtt tctgaacaac gcacgttgct gagtagaaaa atgcgattta
      301 accaaaaagc ctgctgtaca cttaagaaac aagaaggtgt taaggaggcg ttatgaaaac
      361 gcgtattcat gttgtgcagg gtgatattac caaactggcc gttgatgtga ttgtgaatgc
      421 ggctaatccg tcattaatgg gaggcggcgg cgtcgatggg gccattcatc gcgcagcggg
      481 tccggccctg ctggatgcct gtttaaaagt caggcaacag cagggcgatt gccctacggg
      541 gcatgccgtt attacgcttg caggcgatct tcccgctaaa gccgtagtac acaccgtcgg
      601 gccagtctgg cgtggtggtg aacaaaacga agaccagctt ttgcaggatg cctatctcaa
      661 tagtctgcga ctggtggcgg caaacagcta tacgtcagtg gcatttcctg caatcagtac
      721 tggggtttat ggttaccctc gtgcggcagc ggctgaaatc gcagtaaaaa ccgtttcaga
      781 atttattacc cgtcacgctt tacccgaaca ggtatacttt gtctgttatg atgaagaaaa
      841 cgcccacctc tacgaaagac tccttaccca acaaggagat gaatgatttg ccccggctgg
      901 cgagcgcggt gctgccactg tgttcgcaac atcccggtca gtgtggtctt ttccctctgg
      961 agaaaagtct ggatgcgttt gccgcccggt atcgtctggc cgaaatggca gagcatacgc
     1021 tggatgttca gtattacatc tggcaggacg atatgtcggg tcggttactg ttttccgccc
     1081 tgttagtcgc agcaaagcgt ggcgttcgcg tccgtttgtt gctggacgac aacaatacgc
     1141 ccggacttga cgatatttta cgcttgcttg acagccatcc ccgcattgaa gtccggcttt
     1201 ttaatccttt ctcgtttcgc ttgctgcgtc cgcttggtta tatcaccgac ttttcccgtc
     1261 tcaatcgccg tatgcacaat aaaagtttca ctgtcgatgg cgtggttacc ttggtgggag
     1321 gacgaaatat tggtgatgcc tattttgggg caggggaaga gccacttttt tcggatttag
     1381 atgttatggc aataggaccc gtggtagagg acgttgccga tgatttcgcc cgctactggt
     1441 attgcaaatc ggtttcaccc ttacagcagg tgctggatgt cccggaaggt gaaatggcgg
     1501 atcgcatcga gttacccgcc tcctggcata acgatgccat gacgcatcgt tacttacgcc
     1561 aaatggaatc cagtccgttt ataaatcatc tggttgatgg aacgttgccg cttatctggg
     1621 cgaaaacacg tttattaagt gatgatccgg cgaaagggga gggcaaggca aaacggcatt
     1681 cactgttacc gcagcgcctg ttcgatatca tgggctcacc cagtgaacgc atcgatatta
     1741 tctcttccta ttttgtaccg acacgcgcag gtgtggcgca actcttacgg atggtgagaa
     1801 aaggggtaaa gattgcgatc ctaaccaatt ctcttgccgc taacgatgtt gctgtcgtcc
     1861 atgccggata cgcgcgctgg cgcaaaaaat tgctccgcta tggcgtggaa ttatatgaac
     1921 tcaagccgac gcgtgaacaa agtagtacgt tacacgatcg cggcataacc ggtaattccg
     1981 gtgccagcct gcatgctaaa acctttagca tcgatggtaa aacggtgttt atcggctctt
     2041 tcaatttcga tccacgttcg acattgctca atactgaaat gggcttcgtg atagagagcg
     2101 aaacgctggc acagttaatt gataaacgct ttatacagag ccagtatgat gcggcctggc
     2161 agctccgtct ggacaggtgg ggacggatca actgggttga tcgtcatgca aagaaagaga
     2221 ttattctcaa aaaagaaccc gccaccagtt tctggaagcg ggttatggtc agactggcgt
     2281 cgatattgcc cgtggaatgg ttattgtaaa tgtggcttaa cgggctggtg ctttatcgtt
     2341 ctcacgctta acaaccggtt taccagaaaa caaaaacttc agtaacggga tgcgtaaatg
     2401 aatttcatac agaattatcg caatccctac tacgaatatc aggccacaga gaaaaccaag
     2461 ccagttggag gtgatgtgcg gtgtaatgta tgcgccgaaa aacagcgtta acgggtggtg
     2521 aaccagatag ataaatagcg atgcgttaac aaagtaagtc acccgcgctg actggaagtt
     2581 aagcaaacgg tggccaaagg agaagaccac attcaccatc cacagaccga ggaccatggt
     2641 gatcaccgac tcggtttcgt acatccaggc atcgccactg ccatagcgtt ggttgagtaa
     2701 ataagcgaca aatgccaatg ctgctgcaag ggtacagcca cgagacggcg tggtaaacaa
     2761 ggctttaaga tgagggaaaa tgaaagccag tgcaccgagg ataaagaacg gcaaataaaa
     2821 tagcgtttgc atgacaataa aattgaacat gccattactc agaatgggcg gatacacaat
     2881 aaaaatcgtt cttcttatta ccgcatagcc gatgccgagg cataaaaaaa tcaccgatag
     2941 ttttaccatc gagaattttt tattcgtttt atcagaattt tctaaattat ttctgatgcg
     3001 tttaaatatc catacgcaca gcgtcgtcat gactactaac accagtaaaa accacaggtg
     3061 tgatattaat tcccaggcca acgtattata tttgtcatac aatgacagcc caggccaact
     3121 ttccgctttc cctttgacgt attgcagcat aataaattgc ggcaatgtca gtaaggggat
     3181 ggctgttaac atcgggatac ctacacgttc gacacgtact ttccaccatt ttttcaaggg
     3241 atagcgtaaa aaaagcatgt aggaaaagta gccggatata acgaaaaata ccagcatgcg
     3301 gaacgagtgg atgaagtcat taaaaagggt cagccacaat gacggttcgg cgctattcac
     3361 atgccatgta tggctcgaat agattaaaga aatatgaaaa gggatcccta acaacatcag
     3421 ccaggcgcgg atggagtcga ggaaatattc acgttgcgcg ggtactggat tcatatatgg
     3481 ttaactaatc tcggattttt cgtcttatcc ctgtcgggtt atgcctttag gcttgttgcc
     3541 atagcgacac cgacctgacc gcgccaggcg caggcttcaa ggtttttatg catagcataa
     3601 tcgctaccac taaccagaat ggaagcgtct gtaagacggt tgataaataa aattgctggc
     3661 aaaccctaca cgaagtcgat gcttctgtct ttaggagaag cacggaaagt gaaaacggtt
     3721 gcaatcaggt gcttaatcca tgagccagcg tgctgaacga taccgggatt ctgttgtcgg
     3781 aatggctggt tatccattaa aatagatcgg atcgatataa gcacacaaag ggggaagtgc
     3841 ttactaatta tgaaacataa actacaaatg atgaaaatgc gttggttgag tgctgcagta
     3901 atgttaaccc tgtatacatc ttcaagctgg gctttcagta ttgatgatgt cgcaaagcaa
     3961 gctcaatcct tagccgggaa aggctatgag gcgcccaaaa gcaacttgcc ctccgttttc
     4021 cgcgatatga aatacgcgga ctatcagcag atccagttta atcatgacaa agcgtactgg
     4081 aacaatctga agaccccatt caaactcgag ttctaccatc agggtatgta cttcgatacc
     4141 ccggtcaaaa taaatgaagt gactgccacc gcagtcaaac gaatcaaata cagcccggat
     4201 tatttcactt tcggcgatgt tcagcatgac aaagacacgg taaaagacct tggttttgcc
     4261 ggttttaaag tgctttaccc gatcaacagc aaagataaaa acgatgaaat cgtcagcatg
     4321 ctcggggcca gctatttccg cgtgattggt gcaggtcagg tttatggcct ttctgcccgc
     4381 ggcctggcaa ttgataccgc cttgccatcg ggtgaagaat ttccacgctt caaagagttc
     4441 tggatcgagc gtccaaaacc gactgataaa cgtttaacca tttatgcatt gcttgactcg
     4501 ccgcgcgcga caggtgctta caaattcgtg gttatgccag ggcgtgacac ggttgtggat
     4561 gtgcagtcga aaatctatct gcgcgataaa gtcggcaaac tggggggtgc accgttaacc
     4621 agtatgttcc tgtttgggcc gaaccaaccg tcgcctgcaa ataactatcg tccggagttg
     4681 cacgactcta acggtctctc tatccatgcc ggtaatggcg aatggatctg gcgtccgttg
     4741 aataacccga aacatttagc ggttagcagc ttctcgatgg agaacccgca aggcttcggt
     4801 ctgttgcagc gcggtcgtga tttctcccgc tttgaagatc tcgatgatcg ttacgatctt
     4861 cgtccaagcg catgggtgac tccgaaaggg gagtggggta aaggcagcgt tgagctggtg
     4921 gaaattccaa ccaacgatga aaccaacgat aacatcgtcg cttactggac gccggatcag
     4981 ctgccggagc cgggtaaaga gatgaacttt aaatacacca tcaccttcag ccgtgatgaa
     5041 gacaaactgc atgcgccaga taacgcatgg gtgcaacaaa cgcgtcgttc aacgggggat
     5101 gtgaagcagt cgaacctgat tcgccagcct gacggtacta tcgcctttgt ggtcgatttt
     5161 accggcgcag agatgaaaaa actgccagag gataccccgg tcacagcgca aaccagcatt
     5221 ggtgataatg gtgagatagt tgaaagcacg gtgcgctata acccggttac caaaggctgg
     5281 cgtctggtga tgcgtgtgaa agtgaaagat gccaagaaaa ccactgaaat gcgtgctgcg
     5341 ctggtgaatg ccgatcagac gttgagtgaa acctggagct accagttacc tgccaatgaa
     5401 taagacaact gagtacattg acgcaatgcc catcgccgca agcgagaaag cggcattgcc
     5461 gaagactgat atccgcgccg tacatcaggc gctggatgcc gaacaccgca cctgggcgcg
     5521 ggaggatgat tccccgcaag gctcggtaaa ggcgcgtctg gaacaagctt ggccagattc
     5581 acttgctgat ggacagttaa ttaaagacga cgaagggcgc gatcagctga aggcgatgcc
     5641 agaagcaaaa cgctcctcga tgtttcccga cccgtggcgt accaacccgg taggccgttt
     5701 ctgggatcgc ctgcgtggac gcgatgtcac gccgcgctat ctggctcgtt tgaccaaaga
     5761 agagcaggag agcgagcaaa agtggcgtac cgtcggtacc atccgccgtt acattctgtt
     5821 gatcctgacg ctcgcgcaaa ctgtcgtcgc gacctggtat atgaagacca ttcttcctta
     5881 tcagggttgg gcgctgatta atcctatgga tatggttggt caggatttgt gggtttcctt
     5941 tatgcagctt ctgccttata tgctgcaaac cggtatcctg atcctctttg cggtactgtt
     6001 ctgttgggtg tccgccggat tctggacggc gttaatgggc ttcctgcaac tgcttattgg
     6061 tcgcgataaa tacagtatat ctgcgtcaac agttggcgat gaaccattaa acccggagca
     6121 tcgcacggcg ttgatcatgc ctatctgtaa cgaagacgtg aaccgtgttt ttgctggcct
     6181 gcgtgcaacg tgggaatcag taaaagccac cgggaatgcc aaacactttg atgtctacat
     6241 tcttagtgac agttataacc cggatatctg cgtcgcagag caaaaagcct ggatggagct
     6301 tatcgctgaa gtcggtggcg aaggtcagat tttctatcgc cgccgccgtc gccgcgtgaa
     6361 gcgtaaaagc ggtaatatcg atgacttctg ccgtcgctgg ggcagccagt acagctacat
     6421 ggtggtgctg gatgctgact cggtaatgac cggtgattgt ttgtgcgggc tggtgcgcct
     6481 gatggaagcc aacccgaacg ccgggatcat tcagtcgtcg ccgaaagcgt ccggtatgga
     6541 tacgctgtat gcgcgctgtc agcagttcgc gacccgcgtg tatgggccac tgtttacagc
     6601 cggtttgcac ttctggcaac ttggcgagtc gcactactgg ggacataacg cgattatccg
     6661 cgtgaaaccg tttatcgagc actgcgcact ggctccgctg ccgggcgaag gttcctttgc
     6721 cggttcaatc ctgtcacatg actttgtaga agcggcgttg atgcgccgtg caggttgggg
     6781 ggtctggatt gcttacgatc tcccgggttc ttatgaagaa ttaccgccta acttgcttga
     6841 tgagctaaaa cgtgaccgcc gctggtgcca cggtaacctg atgaacttcc gtctgttcct
     6901 ggtgaagggt atgcacccgg ttcaccgtgc ggtgttcctg acgggcgtga tgtcttatct
     6961 ctccgctccg ctgtggttta tgttcctcgc gctctctact gcattgcagg tagtacatgc
     7021 gttgaccgaa ccgcaatact tcctgcaacc acggcagttg ttcccggtgt ggccgcagtg
     7081 gcgtcctgag ctggcgattg cactttttgc ttcgactatg gtgctgttgt tcctgccgaa
     7141 gttattgagc attttgctta tctggtgcaa aggaacgaaa gaatacggcg gcttctggcg
     7201 cgttacatta tcgttgctac tggaagtgct tttttccgtg ctgctggctc cggtacgcat
     7261 gctgttccat acggtcttcg ttgtcagcgc gttccttggc tgggaagtgg tgtggaattc
     7321 accgcagcgt gatgatgact ccacttcctg gggtgaagcg ttcaaacgcc acggctcaca
     7381 gctgctgtta gggttagtgt gggctgttgg gatggcgtgg ctggatctgc gtttcctgtt
     7441 ctggctggca ccgattgtct tctcgttgat cctgtcaccg tttgtttcgg tgatttccag
     7501 ccgtgccacc gttggtctgc gaaccaaaca ctggaaactg ttcctgatcc cggaagagta
     7561 ttcaccgccg caggtgctgg ttgataccga tcgcttcctt gagatgaatc gtcaacgctc
     7621 ccttgatgat ggttttatgc acgcggtgtt taacccgtca tttaacgctc tggcaaccgc
     7681 aatggcgacc gcgcgtcacc gcgccagtaa ggtgctggaa atcgcccgtg accgccacgt
     7741 tgaacaggcg cttaacgaga cgccagagaa gctgaatcgc gatcgtcgcc tggtgctgct
     7801 aagcgatccg gtgacgatgg cccgtctgca tttccgtgtc tggaattccc cggagagata
     7861 ttcttcatgg gtgagttatt acgaagggat aaagctcaat ccactggcat tgcgtaaacc
     7921 ggatgcggct tcgcaataaa aacgtagttg cctgatgcgc tacgcttatc aggcctacat
     7981 cgttcctgca atttattgat tttgcaagat tttgtaggtt gggtaaggcg ttcacgccgc
     8041 atccggcaaa aacaacgagc caataaaaa
//
LOCUS       DAJTOR010000181         7910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTOR010000181 DAJTOR010000000
VERSION     DAJTOR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.181"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(123..953)
                     /locus_tag="OO249_003551"
     CDS             complement(123..953)
                     /locus_tag="OO249_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5637709.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OO249_003552"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OO249_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5637710.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OO249_003553"
     CDS             2379..2729
                     /locus_tag="OO249_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5637711.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OO249_003554"
     CDS             2855..3415
                     /locus_tag="OO249_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5637712.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OO249_003555"
     CDS             3418..6384
                     /locus_tag="OO249_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5637713.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OO249_003556"
     CDS             6451..6828
                     /locus_tag="OO249_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5637714.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OO249_003557"
     CDS             complement(7029..7688)
                     /locus_tag="OO249_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5637715.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2242 c         2133 g         1782 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
//
LOCUS       DAJTOR010000182         7903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTOR010000182 DAJTOR010000000
VERSION     DAJTOR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.182"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(122..853)
                     /gene="dnaQ"
                     /locus_tag="OO249_003558"
     CDS             complement(122..853)
                     /gene="dnaQ"
                     /locus_tag="OO249_003558"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5637716.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            918..1385
                     /gene="rnhA"
                     /locus_tag="OO249_003559"
     CDS             918..1385
                     /gene="rnhA"
                     /locus_tag="OO249_003559"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5637717.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1382..2104)
                     /locus_tag="OO249_003560"
     CDS             complement(1382..2104)
                     /locus_tag="OO249_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5637718.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2138..2893
                     /gene="gloB"
                     /locus_tag="OO249_003561"
     CDS             2138..2893
                     /gene="gloB"
                     /locus_tag="OO249_003561"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5637719.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            2965..4323
                     /gene="mltD"
                     /locus_tag="OO249_003562"
     CDS             2965..4323
                     /gene="mltD"
                     /locus_tag="OO249_003562"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5637720.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4372..4968)
                     /locus_tag="OO249_003563"
                     /pseudo
     CDS             complement(4372..4968)
                     /locus_tag="OO249_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(4999..5799)
                     /locus_tag="OO249_003564"
     CDS             complement(4999..5799)
                     /locus_tag="OO249_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5637721.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6040..6954
                     /locus_tag="OO249_003565"
     CDS             6040..6954
                     /locus_tag="OO249_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5637722.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(6951..7744)
                     /gene="dkgB"
                     /locus_tag="OO249_003566"
                     /pseudo
     CDS             complement(6951..7744)
                     /gene="dkgB"
                     /locus_tag="OO249_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
BASE COUNT         2106 a         1898 c         1919 g         1980 t
ORIGIN      
        1 cgttgggaac gaggcgaata ttacggattg cctcaccctt cgtcaacggt ttttctcatc
       61 ttttgaatcg tttgctgcaa aaatcgccca agccgctatt tttagcgcct tttacaggta
      121 tttatgcccg ccagaggcag cttccgccct tcttctccac cagatcaaga cgggcttcat
      181 gagctgcaag ctcttcatct gtcgcaaaaa caacgcgtaa cttacttgcc tgacgtacaa
      241 tgcgctgaat tgttgcttca ccttgttgct gctgtgtctc tccttccatc gcaaaagcca
      301 tcgacgtttg accaccggtc atcgccagat aaacttccgc aaggatctgt gcatcgagta
      361 atgccccgtg tagcgttcgt ttactgttat ctatttcgta gcgggcacat aacgcatcga
      421 ggctgttgcg cttaccagga aacattttcc tcgccaccgc aaggctatcg gtgaccttac
      481 agaaggtatt ggtcttcgga atatcgcgct taagcaacga aaactcgtag tccataaagc
      541 cgatatcgaa cgctgcgtta tggatcacca actccgcgcc gcggatatag tccatgaact
      601 catcggctac ttcggcaaac gtgggcttat cgagcaaaaa ttcatcggca ataccatgta
      661 cgccaaaggc ttccggatcc accagccgat cgggtttgag ataaacatgg aagttattgc
      721 ccgtcaggcg acggttcacc acttcaacgg caccaatctc aatgatcttg tggccttcat
      781 agtgcgcacc aatctggttc ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa
      841 ttgcagtgct catagcggtc atttatgtca gacttatcgt tttacagttc gattcaatta
      901 caggaagtct accagagatg cttaaacagg tagaaatttt caccgatggt tcgtgtctgg
      961 gcaatccagg acctgggggt tacggcgcta ttttacgcta tcgcggacgc gagaaaacct
     1021 ttagcgcagg ctacacccgc accaccaaca accgtatgga gttgatggcc gctattgtcg
     1081 cgctggaggc gttaaaagaa cattgcgaag tcattttgag taccgacagc cagtatgtcc
     1141 gccagggtat cacccagtgg atccataact ggaaaaaacg tggctggaaa accgcagaca
     1201 aaaaaccagt aaaaaatgtc gatctctggc aacgtcttga tgctgcccta gggcaacatc
     1261 aaatcaaatg ggaatgggtt aaaggccatg ccggacaccc ggaaaacgaa cgctgtgatg
     1321 aactggctcg tgctgcggca atgaatccca cactggaaga tacgggctac caagtcgaag
     1381 tttaagcttg tggtttacga cattgccggg tggctccaac cgcttggcga attcgtggct
     1441 tgtttttact ctgtttcatc gggtttagcg ttaaaggaat agtccgtttc cgggcaacaa
     1501 taagttgtaa gcaaccaagc gcaggaatat gcgcattcaa tagttttcct ccgtgtttgt
     1561 tccacgggag aacgtggaaa cggctggcgt gcagcacttc aaaattcaac aaagagagcc
     1621 agtccagttg ccgcatcaga gtaaacatcc ggctgttata gggcgaggtt tttcgcaata
     1681 ccggcacaag tttgcgtaat cccattaaac taatgggatt gaagccacta atcaccagcc
     1741 agccatcatc aatcaatacc cgatcggttt cacgcaataa acgatgcgga tcggtgcacc
     1801 acggcaatgt atgtgccagt agacaaacat caacggattt atcggcaaaa ggaagatgaa
     1861 gtggatccgc ctgcacctgg acgggcattc cttgcgcaga aacattcact tgatgagaaa
     1921 ccgcgcacgc ttcgcaattg atttctgcgc ttaaattgcc aatcttaagc agatgaaaac
     1981 catacatttt agtgaaccat gggttgagct ggcgctccag cgccttgcga taaagctctc
     2041 cccagggcaa atcgccccag cgatcaggag ccacgacagt ttgagggact cttgccggtt
     2101 tcatcacaac cttccgtttc acactaagag gtaatctatg aatcttaaca gtattcccgc
     2161 ctttgatgac aattacatct gggttttgaa tgatgaagca ggtcgctgcc tgattgtcga
     2221 tcccggagac gcagagccag tattaaacgc cattgccgcc aataactggc aaccggaggc
     2281 catatttctc acccaccatc atcacgatca cgttggcggc gtaaaagaac tggtggaaaa
     2341 gtttccgcaa attgtggtgt atggtccaca agagacacaa gataagggaa caacacaggt
     2401 agtcaaagat ggcgaaactg ccttcgtttt ggggcatgaa tttagtgtaa ttgccacacc
     2461 gggtcacact ttaggacata tctgttactt cagtaaacct tatctatttt gcggcgacac
     2521 gctgttttct ggtgggtgtg gtaggttgtt tgaagggaca gcatcacaaa tgtatcaatc
     2581 acttaataag ttaagtgcgt tacctgacga tacattggta tgttgtgctc atgaatatac
     2641 gttatcaaat atgaagtttg ctttgagtat tcttccgcac gatttgtcca taaatgatta
     2701 ttatcgtaaa gttaaggagt tacgggcaaa aaatcaaata acactacccg taattctgaa
     2761 aaatgagcgg caaattaatg tttttttaag aacggaagat attgatttaa ttaatgtaat
     2821 taatgaagaa acattattgc aacaacctga agagcgtttt gcatggttaa ggtcaaagaa
     2881 agataggttc tgataaaact ttcttgtcat cggctccgtt cgccgttatg atcggtcgtc
     2941 ttttaagcaa ctattgacac acacatgaag gcaaaagcga tattactcgc ctctgtcctg
     3001 ctcgtgggtt gccagagtac cggcaacgtt caacagcacg cacagagcct ttctgcagct
     3061 ggtcaagggg aagcagcaaa gtttacaagt caggcacgat ggatggacga tgggacgtct
     3121 atcgcgccag atggtgactt gtgggctttc attggcgacg agctaaagat gggaattccg
     3181 gaaaatgacc ggattcgcga acagaaacag aaatatttac gcaataagag ctatctccac
     3241 gatgtaactt tacgggcaga gccgtatatg tactggatag cagggcaagt taaaaaacgt
     3301 aacatgccta tggaactggt actactaccc atagtggaga gcgcttttga tcctcacgca
     3361 acgtctggcg ccaatgccgc aggcatctgg cagatcattc cgagcacggg gcgcaattat
     3421 ggtttgaaac agacccgcaa ttatgacgcg cgtcgcgatg ttgttgcttc aacaactgcc
     3481 gcgctgaaca tgatgcagcg tctgaacaag atgtttgacg gcgactggct tctgaccgta
     3541 gcggcttata acagcggcga aggtcgggtc atgaaggcaa ttaaaacgaa caaagcgcgt
     3601 gggaaatcca cggacttctg gtcgttaccg ttgccgcagg aaacgaagca gtacgtgcct
     3661 aaaatgctgg cattgagtga tattctcaaa aacagcaagc gttatggcgt acgtctgcca
     3721 acgaccgatg aaagccgtgc tctggcgcgt gtgcacctga gcagcccggt tgaaatggcg
     3781 aaggttgcag atatggcggg gatttccgtc agcaagctga agacattcaa cgctggcgta
     3841 aaaggctcca cgctgggcgc aagtggcccg cagtacgtga tggtgccaaa gaagcatgca
     3901 gatcaactgc gtgattctct cgcttcaggc gaaattgctg ctgtacagtc gacgctggtt
     3961 gccgacaata cgccgcttaa cagccgtgtt tacaccgtac gctctggcga cacgctttca
     4021 agcatcgctt cacgtctcgg cgtaagcacc aaagatttgc agcagtggaa caaactgcgc
     4081 ggatctaagc tgaagccagg ccaaagtttg acgattggtg caggcagtag cgcacagcgg
     4141 ttggcaaaca acagcgatag cattacgtat cgtgtgcgca aaggcgattc gctttcaagc
     4201 attgctaaac gccacggcgt gaacatcaaa gatgtgatgc gctggaacag tgacactgcg
     4261 aatctgcaac caggcgataa gctgacgttg tttgtgaaaa acaacaacat gccagattcc
     4321 tgacaaacca gataataaaa aaggcaccga ttcccccggt gcctttttta tttatgccgc
     4381 tttatgcgct tctaccatga tgatatcact ggtgaaagag ccatcagtct gcaaggaaaa
     4441 atacgttctc acctctgtcg atgcgctctg ttggtaaatg cgaatagcgt ctactaacgc
     4501 ttctggcgta cgcattctca cgacccatga agaaaattcc agcggtaact tatctgtaat
     4561 taaattatca actatcagat tggcttcatt gattaacgtc aaccacgcac cgctggcgta
     4621 gtttcgtacg tgagaggtat cgcgtaatgc ttctaccgcc tgtaaccaga tgtcgcgcac
     4681 tgggtgaccc ggagacatta catccatcac aatcagccta ccgccaggtt tcaatatcct
     4741 attcacttct cgcagtgctg caccaacatc atgccagtga tgggcagaat aacggctgat
     4801 aacaatatca aatgcgttat cggcaaatgg cagacttgcg gcatatccct ggcgggtggt
     4861 gatatttttc agttgccggg cttcggcagc ttgtgccaca acatccagca tgtgggccga
     4921 taagtcatac gccaccaccg cgctcacgtt ttgcgcagcg ccaaacgttg gtctgccctg
     4981 tggcagacct gacatacctt atttatcagg cttgccggga ctgaattcaa cgagtagcgg
     5041 attgtgatcg gaagcgcgcg taaccagtac agaagcttca ctgacgttca gaccacggta
     5101 gaaaacaaaa tcgagcgggc gaccaaacgc ccgacggcgc tgatcatcgg taaaacgcac
     5161 ctggcgcagc gacatttccc gcgcaaagcg atataacgcg ttcatccttc tacggctcca
     5221 ggcattgaaa tctcccgcca taatgaccgg gccgctgtgg tgagctatct gatcaccaat
     5281 aggaagtaac tgcttactat agacatccac gcccagactg aagttgacgg cgtgtatatt
     5341 aaccaccatc aacaggcggg tgtcaggtaa tggatagacc gtcaccagtg ccgacttcgc
     5401 cagacgcaaa atgggttctc gttcacgtaa cgggcagcaa tacactggat gtgccgccga
     5461 aagggtcatt acgccagaag gatgttgtgg cagcacgaaa gcgggtacct gatcggcggc
     5521 aagatagtta gcggtcgcaa actgtactaa ctctggcgtt gtctgcgctt cctgcaataa
     5581 caccaggtgt gcatctttgc cgtagttctt taataccgac aaccattcag cgcgttgctg
     5641 tttgtatatg ttccacacca ggatccgaat acgctcttcg gtacttaacg gttccccagg
     5701 tggtaatgcc tggccgatgc tcgcaaaaga ccccggcggt aagatccttt ccgcaggttg
     5761 tccggcaaca tagcgcatgg cataggtgtt ttttcgcact tttgactttg aaacctctat
     5821 tacgtcaacc cttccgcact cgggagggtt ctactattat agggatttta gctcactctt
     5881 tcaacgagca attgtatatt gttatgtaag caagtgctta caccggtcag aattttgctc
     5941 attatggaga acattaaatc gcatgacata tcacgcatcc tgatggttta atgacaaata
     6001 aattcaaaat atctcttcta ttttgcaaca ggagcaaaaa tgaaagccac gtcggaagaa
     6061 ctcgccattt ttgtttcggt cgtcgaaagc ggcagcttta gccgggcagc ggaacaatta
     6121 gggcaagcaa actcagcggt aagccgggcg gtgaaaaagc tggagatgaa acttggcgtt
     6181 agcctgctta atcggaccac gcgacaactt agcctgacgg aagaaggcga gcgttatttt
     6241 cgtcgcgtac agtcaatttt gcaggagatg gcagcggcag aatcagaaat tatggagacg
     6301 cgtaatacac cgcgtggact gttacggatc gatgccgcaa ctccagtggt gctgcacttt
     6361 ctgatgccgt taattaagcc tttccgtgaa cgctatccgg aagtcacttt gtcactagtc
     6421 tcctccgaaa cgattattaa tttgatcgaa agaaaagtgg atgtcgcgat acgcgctggt
     6481 acgttaacgg attccagctt acgtgccagg ccgttattta acagttatcg aaaaattatc
     6541 gcctcccccg attatatttc ccgctacggg aagccagaaa cgatcgacga tttaaagcaa
     6601 catgtttgcc tgggattcac tgaacccgct tccctcaata cctggccgat agcctgtagt
     6661 gatggacaat tacatgaggt gaaatacggt ttgtcatcca atagtgggga aacactgaaa
     6721 cagctttgcc tgagtggcaa cgggattgcg tgtttgtccg actacatgat cgacagagaa
     6781 atcgctcgcg gagaattggt ggagttaatg gcagataaag tgttgccagt ggaaatgcca
     6841 ttcagtgccg tctattacag cgaccgtgcg gtaagtacgc gcatccgggc ttttatcgat
     6901 ttccttagcg agcatgtaaa aacagctccc ggaggagctg tcagagaggc ttaatcccat
     6961 tcaggagcca gaccttccgg gctaaccagg cggtcgttgc aatccagtgc ggcgatcgct
     7021 tttttatctt cggcatcaag ctgtaaattt tgtgccttaa gattactttc caggttttta
     7081 cgtttagtag aagaaggaat tactgagtaa ccttccccca tagcccacgc cagaatcact
     7141 tgtgccggag tcgcattgtg tttagctgcg atacgagcaa taacctcatc tttcagggcc
     7201 ttaccatacg ccagcgtcat ataggaagta atatgtatgc cgtgctgttt agcccaggca
     7261 accactttac ggttttgcag ataaggagag agttcaatct ggttagtagc gatgttttca
     7321 gcgccaacag cagcaatcgc cttttccatc aatgggatcg tgaagttgga aataccgatc
     7381 tcacgcgtca gcccttgttt tttggcttcc agcagcgcct gcataacccc ttcaacagag
     7441 acttcatcgt ttggtgacgg ccagtggatt agcgtcagat caacataatc ggtacgcaat
     7501 ttttgcaggc tctctttcag acttgggatc aatttgtctt tgctgagatt ttcaatccag
     7561 attttagtgg tgatgtagag ttcatgacgt ggcacgccac tttctgcaat cgcctgacct
     7621 actgcggctt cgttatcata gatttgtgcg gtatcaattg cgcgataacc aagttcaagc
     7681 gccgttttca cagatgaaat aacaacgtag tctttcaacc taaaccaaat gcagggatag
     7741 ccataatatg cctcttttaa tgctattttc gttaactgag gtgattatgg gcttcagact
     7801 gttgaggaaa aaagcgtaaa atgcagagga tttttgcgat tctggcaata atagatatac
     7861 agacgaaaaa aaacctcgag cgttaactcg aggcttctta ata
//
LOCUS       DAJTOR010000183         7866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTOR010000183 DAJTOR010000000
VERSION     DAJTOR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.183"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(498..860)
                     /locus_tag="OO249_003567"
     CDS             complement(498..860)
                     /locus_tag="OO249_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5637723.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            1145..1354
                     /locus_tag="OO249_003568"
     CDS             1145..1354
                     /locus_tag="OO249_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637724.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1366..1953)
                     /gene="mtlR"
                     /locus_tag="OO249_003569"
     CDS             complement(1366..1953)
                     /gene="mtlR"
                     /locus_tag="OO249_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5637725.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1953..3101)
                     /gene="mtlD"
                     /locus_tag="OO249_003570"
     CDS             complement(1953..3101)
                     /gene="mtlD"
                     /locus_tag="OO249_003570"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5637726.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(3331..5244)
                     /gene="mtlA"
                     /locus_tag="OO249_003571"
     CDS             complement(3331..5244)
                     /gene="mtlA"
                     /locus_tag="OO249_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5637727.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLT
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5781..6143
                     /locus_tag="OO249_003572"
     CDS             5781..6143
                     /locus_tag="OO249_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5637728.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6146..7282
                     /locus_tag="OO249_003573"
     CDS             6146..7282
                     /locus_tag="OO249_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5637729.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGGLQSLTVVPGTDGVQGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT         1964 a         1909 c         1903 g         2090 t
ORIGIN      
        1 ttgtccattg tattattggt gatttatgtt gctttacatt aatgacagtg atgtgtgtcg
       61 attaaatatt gttttaagca ttcatcataa aaatgtatca ttcgtttcaa aagatgtctg
      121 tgtttgtatg aatatattat tttcgtggat tctaatgatg attattaata aatgtcaaat
      181 atgaaagaaa ccatgtctgg gaaaaaattt acagataatt tattgtatga atatgatgat
      241 taaaatttca caattaaatc cgaaaaaaag agacgtaact gcttgttaat taataaatga
      301 tatcttctat atatttttat tgtttatagg tggctatttc ggtaaaaatg cattttcaga
      361 atatcaacca gcggtaaatc gacgtaaagg gagtgaattg tttggtaatt aggcgagtag
      421 acaggagagt ccccgcattg accagctaag gcaagccaga tgcgggttga tatgatcgaa
      481 ccagtcgtct gaggcgctta aaacgcagtc ttagagaatc cagtcatctc ctgcaggccc
      541 atttcacggc ccagcgcggt cattgggtgc acaaccacca gtccgcgaac acttttcttc
      601 agcttgccca tatcagcctg ctcttttttg gtaattgcgc gctgaaacgg catcttcatc
      661 agcttctgtg cttctttact cagtttttgg ctatgaacct cacgcaggcg agcaatttct
      721 gcttccagcg tggctttctc tttttccagc tcggcatatt tgtctgcggc ttcaaccagc
      781 gacagatcgg cctgctggtg gcggatggcg tccaggcgat cgctgagacg tttgatttcg
      841 tttttttcga cttctttcat gtgatggctt ctgataaaga taaatttata gcccctagta
      901 tgcgcgacca ggtgctttgt tcacaactga tgacattgta atagttctgc aacgtaatca
      961 ttaacatgct caaaaactac gcaattcgtt gattaattaa gaaatctata gaaaaaagat
     1021 aataaacagc agcggatgat tattgaggat tcagcacgtt ttccttaatg tcatctttac
     1081 cgggaaccta aactaaaatt ggtggccgcg cattcccggc gcggggtaat acggagatat
     1141 catcatgggc aaattaggtg aaaacgttcc gcttcttatc gataaagccg tagatttcat
     1201 ggcatcaagc caggcgttcc gggagtatct gaaaaaactt cctccccgta acgcgattcc
     1261 gtccggaata cccgatgaaa gcgtgccgtt atatctacaa cgtctggagt attatcgtcg
     1321 gctttatcgg ccgaagcagg tagaggggca gtaagcagac gttgcttact tttgaaaggc
     1381 tttttttaag ctgattttgg aaatcagctc agtcagtgaa aggaccattg ttgatcgcac
     1441 agcctgttga tagcgctgaa tttgcattgc atacaaactg gagtctgctg gttcaaactg
     1501 tggcggcggt ggtaacgccg ccacgcagtg cagttcacca aagggtccaa ggatttcgtc
     1561 gtcggtaaag gcgtactcgt tgccgtcgtg atttagctct tcacgcaatg ccatcagcag
     1621 ttccgcatct tcgtattcct ggcggttaat gacgcccaac ccgtaaatga gttttaaacg
     1681 cacagaaaga tcgcccagcg gaccgtcgcc gtcgagtaac ggttctacag catacttcac
     1741 cgcgtaatcg tctttgcgga atacctgaag caccagaaga tttaccgcct cggtcaaaag
     1801 ctcgacggcg gtgatcagaa agcttcgcac ggttttgcca gcattcagac gctcaagcac
     1861 acggttttca aaggcctggg tttgttccat tgttgcctgc atatctgaca atctgttatt
     1921 cgggcgcagg gtgtcctgcg cctggtccac cattattgca ttgctttata agcggttacc
     1981 gcctcggata caacctcact gttggcatca agaccggaaa tctgtgccag cgccgcctgc
     2041 ggacctttgt cagcgatcag tgctgccagt tcctgagcct gcggatcatc ttcactgcgg
     2101 aagtgcattg caccagcaat cccctgaatc agattcttgt gcggaaggct gtattccagc
     2161 gtaccgagca gtggcttgat cagacggtcg ccagcactca gtttacgcag cggctgacgg
     2221 cctacgcgct ctacatcatc tttcagatac gggttctcaa aacgaccgag gattttctgg
     2281 atgtacgccg catgtttgtc tgcgtcaaag ccgtagcgct tgatcagtac cgcaccactt
     2341 tcttccatcg caccttttac caccgcgcgg attttctcgt cgagaatagc gtcacgaatg
     2401 gtctgatgac cggccagttt tccgaggtac gcggttatag catgacccgt gttcagggtg
     2461 aagagtttac gttcgacaaa tgccatcagg ttgtcggtta actccatgct tgggatgttc
     2521 ggcagtgcgc ctttgaactg cgttttatcg acaatccatt cgctgaaagt ttctaccgtc
     2581 acttccagcg gatcgttagt tgccgaagcc gaaggcggta cgatgcggtc aacggcggaa
     2641 tcgacaaagc caacgtgttc ttctacccac gctttggcgt cttccggcag ggcgttcatc
     2701 acatggcctt tcagctgcgt ggtaccgcgt accatgtttt cacaggcgat gatgttcagc
     2761 ggggattcat taccttgttc tttgcgtttc accagccctt tggcgatagc cggagcaata
     2821 cgttccagca caaccgggcc aacggcggta gtgactaaat cgacctgagc aatcagatca
     2881 acgacatcat caccaatgct gctgacagca ttgacgccgg aaacggtatc tacctgctcg
     2941 gtttcaccaa ccacatgaac ctgatagcta tgacgggcat tcagggcatc aagtaccacc
     3001 tgattgacat cggcaaacgt cagttggata cccgcgtctg ccagcagttt accgataaag
     3061 ccacgaccga tattacctgc gccaaaatgt aatgctttca tagtattaac cttcatcaat
     3121 gtttttaccc gagagggctg gggtgaggct tttccctcac ccaaaccctc tccgtgagga
     3181 gaggggcagc gccggatgcg gcgtaagcgc cttatccggc ctacaagaac gtgcaaattc
     3241 aacaaatcgt gatgcatcct gtaggcctga taagcgtagc gcatcaggca attaacccca
     3301 ccttctccat gtggagaggg tgggattgga ttacttacga cctgccaaca gttccagcac
     3361 ttcatccacg ctggtggtgt gtgccagacg ctcgatgacg gactcatcat ccagtgcatt
     3421 ggtcaggctg gtgataacct gaatgtgctc gttgttacgg gctgcaatac caatcaccag
     3481 acgggcaatg tcatcttcct cttcaccgaa gcgcacgcct tccgggtact ggcagaacac
     3541 cacgcccgtt ttcagtacgc gatctttcgc ttcaaccgta ccgtgtggca ccgcgataga
     3601 ctcacccaga taagtcgggg tcagtttttc acgatccagc atcgcctgaa cgtattccgg
     3661 ctcaacgtaa ccgcctttca ccagctgctc gccagcaaaa cgaatcgctt cttctttggt
     3721 tgctgctttg cgaccgagga agatgttctc cgcgcccagt ttgaacaggt tagcactgga
     3781 atcgtcaaag ctgtctttca gactgtcttt cactttcact tcgttttcag tatggcgctg
     3841 ggcagcaacc agacgttcgg ttaggctggt gtacaggccg ctgtcgagga agttggtcag
     3901 cgaaatatgc tgtgcctgcg gaacctggcg catagcgcgt tcggtcaggt cacggtgagt
     3961 gatgacgagg tccacatctg gcggcaggtt gttgatcgcg ctgttagtga ccgaaatctg
     4021 cgacagacct gcatcctgaa ttttcttacg cagcacgcct gcgcccatcg cacttgaacc
     4081 cataccggca tcacaggcaa cgatgatttt acgtacgtgg ctcaggtcgt tagtcacatc
     4141 gcccgcagac agcggagatg tgcctttaga ctcagctttc atgtcctgca tacgacgagt
     4201 tgctgcttca atatcatctt cttctttcac tttgctggtt ttcagcaaaa tagcagagac
     4261 aacgaaggag acagccatcg ccgcacacac acccgcgatg ttagcgaaat aagccccttt
     4321 tggtgtcatc gccagtacag caaggataga acccggagat gccggagaaa ccagaccacc
     4381 gcccaggata gtcagcgtga acacgccagt cataccgccg aggatgactg cgaggatcag
     4441 acgcggattc atcagcacat acgggaagta gatttcgtgg ataccaccca ggaagtggat
     4501 gattgccgca ccgcccgcag actgtttagc gctaccacga ccaaagaaca tgtacgccag
     4561 cagcacgccc atacctggac ctgggttagc ttcaatcagg aagaagattg atttacccag
     4621 ttcatgggac tgctgaatac ccagcggcga gaagataccg tggttaatgg cgttgttgag
     4681 gaacaggatt ttcgccggct caacaaagat agacgccagc ggcagcatgt catggacaac
     4741 catgaagtta acgcccgcag ccagcatttt ggacagggct tcaacaatcg ggccaatgcc
     4801 gaggaatgcc agaatagcga ggatcatccc gatgatgcct gcggagaagt tattcaccag
     4861 catctcaaaa ccggatttga tcttaccgtc tacccagcgg tcgaagtgct taatgcacca
     4921 gccgcccagc ggacctgcaa tcatagaacc gaggaacatc ggcatgtctg cgccgacgat
     4981 aacgcccatg gtggtgatgg caccgactac gccgccacgt tcgccgccta ccagcttacc
     5041 accggtataa ccgatcagca gcggcaggag ataagtgatc atcggcccga ccagcttcgc
     5101 cagcgtctcg ttcgttaacc accctgttgg aataaataac gcggtgatga taccccacgc
     5161 gataaacgcg ccgatatttg gcatcaccat gttgctgagg aaacgaccaa agctttgcac
     5221 tttgatctta atatcggatg acataaaaac accccttctt atgtttgctg tcgcgcaggc
     5281 tggaagcccg aggttttttg ttaatgtggc ggcagaggta gccggacctt gtagatactg
     5341 cgaaatctgg cactgaatcg gttaactgtc cagtcgacgg ccttttgtgt gatattaatc
     5401 acataaatat aggaggtaga gaggtaattg acgtgacgtt catcacaaaa cgcttgtgta
     5461 aaaaagcaac aaggcggcga ttttcgctaa aaatggcatt taaatgtgag tagtgtcaca
     5521 tttttgtttt gatggtttgt tgaatctttg tgatctgaat cacaagatat tttcttacgg
     5581 cgttcacctg atgtgatcta cagcatgtta tgccttcgtc atacaatcgt tatgtaacta
     5641 agcaactcat cacctctaaa aattattctt tggtgttatg gcgagaatcg cgaacccgga
     5701 ttattgtcaa aatatcagcc tgactccctt ctggctgcca gcccaaaaac aacactgata
     5761 aacgtgaatc cgaggcagat atgtttctaa actatttcgc gttgggagtg ctgatcttcg
     5821 tctttctggt gattttttat ggaatcatcg cgatacatga catcccttat ctgattgcca
     5881 aaaagcgcaa tcacccccat gccgacgcta ttcatacggc gggctgggtg agcctgttta
     5941 ctctgcatgt tatctggccg tttctgtgga tctgggcgac gctctatcaa ccggagcgtg
     6001 gctggggtat gcagtcacat gttgcgtcgc aggagaaagc gactgacccg gaaatcgcct
     6061 cactttctga ccgaatttcc cggctggagc atcaactcgc cgccgagaaa aagactgact
     6121 attccacgtt cccggagatc taattatgga tctattgatt gttttaactt acgtggcgct
     6181 ggcgtgggcg gtctttaaaa tcttccgcat tccggtaaat cagtggacgc tggcgacggc
     6241 ggcgctggga ggcgtgtttc tggtgagtgg tttgattttg ttgatgaact acaaccaccc
     6301 ttacactttt accgcgcaaa aggcagtgat agcgatccct atcacgccac aggtgacggg
     6361 aattgttact gaagtcactg acaagaataa tcagcttatt caaaagggcg aggtgctttt
     6421 taagctcgac ccggttcgtt accaggcgcg agttgacaga cttcaggctg acctgatgac
     6481 ggcgacgcat aatataaaga cgctgcgtgc gcagctcact gaagcgcagg ccaacaccac
     6541 ccaggtttca gcggagcgcg accgtctgtt taaaaattat caacgttact tgaaaggcag
     6601 ccaggcggcg gtgaatccgt tctcggaacg tgacatcgac gatgcgcggc aaaatttcct
     6661 cgcgcaggat gcgctggtga aaggctcggt ggcggagcag gcgcagatcc agagccagct
     6721 cgacagtatg gttaacggcg agcaatcgca gattgtgagc ttaagagcgc aacttactga
     6781 agcaaaatat aaccttgagc agactgtcat tcgcgcgccg agcaatggct acgttactca
     6841 ggtactgatc cgcccaggta catacgcagc tgccttgccg ctgcgtccgg tgatggtctt
     6901 catccccgag caaaaacggc aaattgtcgc ccaatttcgg caaaactcgc tgttacgtct
     6961 gaaacccggc gatgatgcgg aagtggtgtt taacgcgcta cctgggcagg tgtttcacgg
     7021 caaactgact agtattttac ctgtcgtgcc aggcggttct tatcaggcgc aggggggatt
     7081 gcaatcatta acggtcgtgc ccggcacgga cggtgtgcag ggaaccattg aactggaccc
     7141 taacgatgat atcgatgcct tacccgacgg catctacgcc caggtggcgg tttactccga
     7201 ccatttcagc catgtttcgg tgatgcggaa agtgctgcta agaatgacca gctggatgca
     7261 ttatctttat ttggatcatt gagggggagg ggggagatta tggaactttg ctccttgtaa
     7321 ggactaatta tttttgcata ttacttcatt agctgcgatg atttttcttt aatattcatt
     7381 atgttaataa gttctggatg agtgtgctct cattggattt tttatccgtc ttgttctgcc
     7441 gcaggatgag actatacatc gtcgagatcg caatgatgat atgagttgtt cctcctgcga
     7501 cggctctaat caaaaaagag caggagtgct tttccatcta ttgcgatgat ggctttatta
     7561 tctgaaggat gacagtcaca tttaaaaaat atttaatcta tagatcacaa tgtagggtca
     7621 tggaatatat gtatacaact attttcttct tcacttttaa ttatcgtatt cattataaaa
     7681 ctaagttaaa aaaactcctg gagagtgagt tattaatata tttttaaata aaattaagtt
     7741 atcattcatc gatgcatttt atagaatcat caatggccat aatagtacta ttaactctca
     7801 attttatatt tttatcatca ctacaaatat taactgaatt tatatcaatg atttttttat
     7861 tcttat
//
LOCUS       DAJTOR010000184         7808 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTOR010000184 DAJTOR010000000
VERSION     DAJTOR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.184"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            258..401
                     /locus_tag="OO249_003574"
     CDS             258..401
                     /locus_tag="OO249_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5637730.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            414..623
                     /gene="cspE"
                     /locus_tag="OO249_003575"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             414..623
                     /gene="cspE"
                     /locus_tag="OO249_003575"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5637731.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            789..1598
                     /gene="rlmA"
                     /locus_tag="OO249_003576"
     CDS             789..1598
                     /gene="rlmA"
                     /locus_tag="OO249_003576"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5637732.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1595..2161)
                     /gene="mntP"
                     /locus_tag="OO249_003577"
     CDS             complement(1595..2161)
                     /gene="mntP"
                     /locus_tag="OO249_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5637733.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2589..3047)
                     /locus_tag="OO249_003578"
     CDS             complement(2589..3047)
                     /locus_tag="OO249_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5637734.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLERIYKLIVLTQ"
     gene            complement(3102..3953)
                     /gene="manZ"
                     /locus_tag="OO249_003579"
     CDS             complement(3102..3953)
                     /gene="manZ"
                     /locus_tag="OO249_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5637735.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3966..4766)
                     /gene="manY"
                     /locus_tag="OO249_003580"
     CDS             complement(3966..4766)
                     /gene="manY"
                     /locus_tag="OO249_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5637736.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4829..5800)
                     /gene="manX"
                     /locus_tag="OO249_003581"
     CDS             complement(4829..5800)
                     /gene="manX"
                     /locus_tag="OO249_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5637737.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6263..>7808
                     /gene="yoaE"
                     /locus_tag="OO249_003582"
     CDS             6263..>7808
                     /gene="yoaE"
                     /locus_tag="OO249_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5637738.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDVDNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDED"
BASE COUNT         1983 a         1911 c         1938 g         1976 t
ORIGIN      
        1 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaaatacgcc
       61 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
      121 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
      181 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac
      241 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
      301 atgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
      361 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
      421 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
      481 ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
      541 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
      601 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
      661 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
      721 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
      781 gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
      841 gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat gtcaatctgc
      901 tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
      961 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
     1021 tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt ggtgaagggt
     1081 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt ctggatgttt
     1141 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt tgtgtcgctt
     1201 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
     1261 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
     1321 cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
     1381 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt
     1441 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttgcct
     1501 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
     1561 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
     1621 tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttccgctttt ttcccaataa
     1681 ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag gttgcacaac
     1741 caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca acacccacag
     1801 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgtcgac
     1861 ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
     1921 cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca aaccggctgg
     1981 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa
     2041 ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg
     2101 atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca
     2161 tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
     2221 catgaacaac aagccgtgat ggttttcgcg ttcatgcagg catagcgatt gatgtgcata
     2281 tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
     2341 gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa
     2401 catattttga gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac
     2461 gaattcattt agatttatat atcaatgaaa cgttaaaata aataacaaat aagggaagag
     2521 gtaatatagc ctaagctatg tctgattatt aaaaatacac taaacatagc tttggctaaa
     2581 ttcataactt attgagttaa aacgataagc ttataaattc tttccagatt gtcgatattt
     2641 cgaacgcgga ttaacagccg acgctgttct aattgcatca ccaacacgcc atcttccgac
     2701 aagttcatcg ctttgattcg gctatattca atccagacat tggcgaagaa aaaacctttt
     2761 tgtttaaaga tgatcttcgg aacgcggatc cagaatatat aaaaacccat cagagccagt
     2821 gcgcttaata accatgtcgt tattaatgca ccatgattcg tgacgttgtt atagataaga
     2881 atgacaatca atccgacgaa gataacgcta tcgatgcgac cacgccggag caaaggaatt
     2941 gccagcaggg tggggccgtt acggcggggc atgatgaact gatcgtagat cgcgaaggcc
     3001 aggagtgcgg cgatgaaaag aatcagtacc aggtccgtga ttgtcattaa tcctccagat
     3061 aaaaaaacgg ggccaaaagg ccccggtagt gtacaacagt cttacagtcc cagcaggccg
     3121 caagcgtaac cagcgatacc gatgacgaag aagccaacga tgatccacag cgggttaact
     3181 tttttgcgca gtagccacat acaagcaaag gtcagcagca gtggtaccag gcctggcatt
     3241 aactggtcca gaatagtctg gacagtagta acgtgttctt tgcccgtctg gtcagtaatg
     3301 cgagagacaa ccagcgggat gttgacatgt gtccacttgt taaccaatgc ccccatgaca
     3361 aacaggccga ggatagacgc cccttccgtc agtttttgca ggaagccacc acccatatct
     3421 ttaacgatat cgataccttt ggagtaacca tacgctacgc cgtagtaacg ggttgccaga
     3481 cgcaccaggt taaacaggat gaagaacagc agcggaccta acaggctgcc gctcatcgcg
     3541 ataccggcac ccagtgctgc aaataccgga cgtacggttc cccagaagat cgggtcgcct
     3601 acaccagcca gcggccccat caaaccgact ttgataccgt tgatagcacc gtcgtcgatc
     3661 tctgcgccat tagcacgctg ttcttccagc gccagggtta cgccgagaat cggcgcagcc
     3721 acgaacggct gggtgttaaa gaactccagg tgacggcgaa tagcttgttt acgagcttcg
     3781 ttgttctcag ggtagaggcg acgaattgcc ggtaccatag agaagcagaa acccagtgcc
     3841 tgcatacgtt cgaagttcca tgaaccctgg aagaggttag aacgcaggaa gacgccacga
     3901 atatcacttt gagtgagttt tttctcggtg gtagtttgag ttgtatcaac catttcgctc
     3961 acctgttagt ccagttcgtt atcgagatcg ttgttaccag cagcctgagc aggcgcaccg
     4021 gctacgcggt tgtatttcgg gctaagttgg atgtagagca ctgccataac agtaccgatc
     4081 acacccagag caaccaggtt aaagttggtg aatgctgcgg ttacgaagcc gaggtagaag
     4141 aacggcatca ggtagccagc acgcatcatg ttgataacca tcgcataacc aaccaccacg
     4201 atcatgccac cggcgatatt cagaccattg gtcaccactt ccggaatcgc attcagcatg
     4261 ttctgtactt cgctggtacc aacagacagc gcaacgatga cggccggaat agccacacgc
     4321 attgcttgca ggaacagaga agaaacgtgg atccaggaaa tcgctgtcag gttgccgtta
     4381 tcagcagcct tatccgcagc gtgctggaaa gcaacggtaa tagtacgaac gatgatggtc
     4441 agtacctggc ccgcagcggc cagagggatt gccagtgcga tacctgcacc aatgctctga
     4501 tgacctgcga taaccagaat ggtagaaatg atagaagcca gagcggcgtc aggcgcaact
     4561 gcagcaccga tgttcatcca gcccagcgcg atcatttcca gcgtaccacc gataataata
     4621 ccggttttca tatccccaag aacgataccc accagggtac acgcgattag cggacggtga
     4681 aactgaaatt catcgaggat tgatcccata cctgcgatac aggctacgat aaataccagc
     4741 acaatttgaa gagtggtaat ctccattgta cttctcctgt ttacgtaaga cttaagtgtg
     4801 aaaactgagt gataatcaac acaatacgtt acttatcgat tttgctgatc agatccatca
     4861 ttttcagttt cggatcggtg gaaaccttac ggacttccag ctcaataccg cgcgcattca
     4921 gtttcttgaa cgcctcgata tctttttcat caaccgaaac cgcgttattc acctgggttt
     4981 taccctgacg gaatgccata ccaccgacgt taacagaggt gattttcacg ccgccttcaa
     5041 cgagacgctc tacatctgtt gggttggtaa ataacagcat tacgcgttcg ccagcatatt
     5101 tcgggttgtt gtagacgcga atcattttgg caacatcaac tacgtgtgct gttacgcccg
     5161 gaggtgcaac ctgggtgagc agtgtcttac gcacggtatc cgcagccact tcatcactaa
     5221 caacaataat acgggagaca ttggtttctt tggtccagcg ggtggcgacc tgaccgtgaa
     5281 tcagacggtc gtcgatacgc gcaaggccaa taaccatgta gtcgtttggc cccattggtt
     5341 ttgccggagt tggagccgct tttggtgctg cggcagcggg tgctggcgcg gctttttcaa
     5401 ccggtttggc tttcagtgct ttcacacctt cacggcctgt ttctactgcc agcgcaacca
     5461 gttcatcaaa gcttgggtca tcatcacgag ccattaacgt ttccacgagc attggaatgt
     5521 taacgcctgc aatgacttca taatgctctt tgtcgacgac aatgcggctg gcagcattga
     5581 acgggctgcc tccccatgta tcaacgagaa acagcacgcc tttagtggtg tcgagttttg
     5641 ccaactgagc gttgtacttt tcaatcagcg tttcggcatt ttcacctgga acgaaatcga
     5701 tccagccgac gttttcctgc tcgcctaaca gcatttctgc cgttttaagc aactgctctg
     5761 cagcccaacc atgtgtgcct ataacaatag caatggtcac ttgctacctc ctttattatc
     5821 gttaacacct caacgtgcca gatgtatttt tgaatcgtcg tccacaatcg aatcgattca
     5881 gataagggct acaaccaaaa acccctgcgt ttcgcgattt attttagata tcgaaaaaat
     5941 tattttatgt gatgaagatc cgtaatttaa ctttcgatta gcagaaattt cgaaaggtaa
     6001 aatatccttg tcacattcgt ttgcaaagga aggtaaatct ttgccaaatc agaggcgtct
     6061 ctgatatgtt taactcccgt ttaacaacca tggagtatag ggcagtagcc cgcagtatgg
     6121 atcgtcaccg acgtcatttc agcatcaggc cttttaacgc ctgcctttct ggcactctat
     6181 gccgcacctt tcgtttgcat tttgtcgtta cgcctgcatt atttctggcg tcgaatagct
     6241 attccttaag caggagcttg tcatggaatt cttaatggac ccctcaattt gggcggggct
     6301 actcacgctt gttgttctcg aaattgtgct gggtatcgat aacctggtct tcatcgccat
     6361 tcttgctgac aaactgccgc caaaacaacg cgataaagcg cgtttgctgg ggttatcact
     6421 ggcgctgatt atgcgtctgg ggctgctgtc gctgatttca tggatggtca cgctgaccaa
     6481 accgctattt accgtcatgg atttctcctt ctccggacgc gacctgatta tgttgttcgg
     6541 ggggatattc ttgctgttca aagcaacaac cgaactgcat gaacggctgg aaaaccgcga
     6601 tcatgattcc ggccacggta aaggctacgc cagtttctgg gtggtcgtca cacagattgt
     6661 catccttgac gccgtcttct cgttggatgc ggtaattact gcagtaggga tggttaacca
     6721 tctgccggtg atgatggcgg cggtagtgat tgcgatggcg gttatgttgc tggcatccaa
     6781 accgctgacg cgattcgtta accagcaccc cacggtggtg gtgctctgtc tgagcttcct
     6841 gttaatgatt ggtcttagtc tggtggcaga aggtttcggt ttccacattc cgaaaggtta
     6901 cctgtatgcc gcgattggct tctcgatcat catcgaagtg tttaaccaga ttgcgcgtcg
     6961 caactttatt cgccaccagt cgactttgcc gctgcgagcg cgtactgccg atgccatcct
     7021 gcgtttgatg ggcgggaaac gtcaggccaa tgttcagcac gatgtcgata acccgatgcc
     7081 gatcccggaa ggtgcatttg ccgaagaaga acgttacatg attaacggcg tactgacgct
     7141 ggcgtcgcgt tctctgcgcg ggatcatgac gccgcgtggt gaaataagct gggttgacgc
     7201 taatctcggg gtcgatgaaa tccgcgagca actgctctct tcaccgcaca gtctgttccc
     7261 ggtatgtcgc ggtgaactgg atgaaatcat cggtattgta cgtgctaaag aactgctggt
     7321 ggcgctggaa gagggcgttg atgtggcggc gattgcttcg gcgtctccgg cgattatcgt
     7381 cccggaaacc ctcgatccga tcaacctgtt gggcgtgctg cgtcgtgctc gcgggagctt
     7441 tgttatcgtg accaacgagt ttggtgtggt acaaggtctg gtcacgccgc tggatgtgct
     7501 ggaagccatt gcgggtgaat tcccggacgc tgacgaaacg ccggaaatca ttactgatgg
     7561 tgacggctgg ctggtaaaag gcggtacaga tttgcatgcc ttgcagcagg cgcttgatgt
     7621 tgagcacctt gccgatgacg atgatatcgc gacggtcgcg ggcctcgtga tctcggcaaa
     7681 tggtcacatt ccccgtgtgg gcgatgtgat tgatgtaggg ccactgcata tcaccatcat
     7741 tgaagccaat gattatcgtg ttgatctggt tcgcattgtt aaagagcaac cggcgcacga
     7801 tgaagatg
//
LOCUS       DAJTOR010000185         7768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTOR010000185 DAJTOR010000000
VERSION     DAJTOR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.185"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..588
                     /locus_tag="OO249_003583"
     CDS             <1..588
                     /locus_tag="OO249_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HCR5637739.1"
                     /translation="GDNKDATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHF
                     QAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQ
                     FEKLSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQL
                     ISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            951..1988
                     /gene="dusA"
                     /locus_tag="OO249_003584"
     CDS             951..1988
                     /gene="dusA"
                     /locus_tag="OO249_003584"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5637740.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2122..2364
                     /gene="pspG"
                     /locus_tag="OO249_003585"
     CDS             2122..2364
                     /gene="pspG"
                     /locus_tag="OO249_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5637741.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2529..3512)
                     /locus_tag="OO249_003586"
     CDS             complement(2529..3512)
                     /locus_tag="OO249_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5637742.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGSGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3595..5010
                     /gene="dnaB"
                     /locus_tag="OO249_003587"
     CDS             3595..5010
                     /gene="dnaB"
                     /locus_tag="OO249_003587"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5637743.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5063..6142
                     /gene="alr"
                     /locus_tag="OO249_003588"
     CDS             5063..6142
                     /gene="alr"
                     /locus_tag="OO249_003588"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5637744.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6395..7588
                     /gene="tyrB"
                     /locus_tag="OO249_003589"
     CDS             6395..7588
                     /gene="tyrB"
                     /locus_tag="OO249_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5637745.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1883 a         1857 c         2030 g         1998 t
ORIGIN      
        1 ggggataata aagatgcgac tcccattgaa caatgtactt ttgatgtaga aactaagaat
       61 ggccttccct ccacggttca gctattttat gaggaatcta aggataatga acctttagcg
      121 aatatacatt ttcaagcaat aggttctggt tttttaacgt ttgttaatgc ctgccaggaa
      181 catgatgaca acagcttaaa attatttgct tcgctgttaa tttcactttc atattctagt
      241 gcctacgcag atttatcaga aacagtgtat attaatgaaa ataatgagag ctacctgaaa
      301 gctcagtttg aaaaattatc tcaacgtgat atgaagaagt acctgggaga gatgaagcgt
      361 ctggctgatg ggggagaaat gaattttgat ggctatctgg ataagatgtc acatctggtg
      421 aatgaaggga cgctcgatcc tgatatttta agcaaaatgc gagatgctgc accacaatta
      481 attagcttcg cgaagtcgtt tgacccaacc tcaaaggaag agattaaaat acttacagac
      541 acttctaaat taatttatga tttgttcggg gttaaatcgg agaaataata tgtgaagttc
      601 ttcgatagta tggaaggcat tatataaaag gacccaatat ttattgggtc cttttttctc
      661 tatcaatgct attagcaggg agatatatca ccaaagttta atgtgtgatt ttttatttat
      721 cgtcgaacct ggattgttta tcattggcct taacaaagtt aacggctaat aagattattt
      781 ccatcacttc gtgagagctt catgccttga gaggatctca attttctttt gcaatgagac
      841 aggcgcttcc tgttgttatg gtatagtacc ccgctattga gcctcctgaa tagtgatgct
      901 gaataacata accccatgat atatcgataa aataatctct acatttgaaa atgcacggta
      961 attctgaaat gcaaaaaatc aaccaaacca gcgcaatgcc tgaaaaaact gacgttcact
     1021 ggagtggtcg gtttagcgtt gcaccaatgc tcgactggac ggacagacat tgccgctatt
     1081 tcttgcgtct gctttcccgc aatacgttgc tgtataccga aatggtgacc acaggggcga
     1141 ttattcacgg taaaggtgat tacctggcgt acagtgaaga agaacatccg gtagcgttgc
     1201 aactcggcgg tagcgatccg gcggcgctgg cacagtgtgc gaagctggca gaagcgcgtg
     1261 gatatgatga gatcaacctg aatgtcggct gcccgtctga ccgggtgcag aacggcatgt
     1321 ttggtgcgtg tctgatgggt aatgcgcagc tggttgccga ttgcgtgaaa gcgatgcgcg
     1381 atgtggtgtc gattccggtg acggtgaaaa cgcgtattgg catcgatgac caggacagct
     1441 atgaatttct ctgcgatttc atcaacaccg tttccggcaa aggcgagtgt gggatgttca
     1501 tcatccatgc acgtaaagcc tggctttcgg ggttaagccc gaaagaaaac cgtgaaatcc
     1561 cgccgctcga ttatccgcgt gtgtatcaac tgaagcgtga ctttccgcat ctgacaatgt
     1621 cgattaacgg tggtatcaag tcgctggaag aggccaaagc acacctgcaa catatggatg
     1681 gcgtgatggt cgggcgcgag gcgtatcaga atccgggtat tctggcggcg gtagaccggg
     1741 agatctttgg ttcctcggat accgatgcct atccggtggc ggtagtgcgc gccatgtatc
     1801 cgtacattga gcgtgaactc agccagggga cgtatctcgg ccatattacc cggcatatgt
     1861 tgggcttgtt ccagggtatt cctggcgcgc ggcagtggcg gcgttattta agtgaaaatg
     1921 cccataaagc gggtgcagac attaatgtgc tggaacacgc gctcaaactg gtggcggata
     1981 agcgttaact tttcaccaaa aagtagtcaa attcaccacg ccctgcgcac cgtcgcgggg
     2041 cgttttgctg ttaaatcaat agattatttt tggcatgatt cttgtaatgc cagcaagaga
     2101 tttcatattt gggagagcat catgctggaa ctactttttg tgattggctt ttttgtcatg
     2161 ctgatggtca ccggcgtttc gttgctgggc attatcgccg cgctggttgt ggcgacggcc
     2221 attatgttcc tcggcggtat gctggcattg atgattaagt tgctgccgtg gttactactg
     2281 gcgattgcgg tggtgtgggt tattaaggcg attaaagcac caaaagtgcc taaatatcag
     2341 cgttatgacc gctggcgtta ctaagggatt gtgcggatga tcacaaccta aggttttatc
     2401 cttagaacaa aataggaatt gataatcaaa tctgtcacta ttgcgcctct aacagattca
     2461 tcgtgctgta ccctacatac agccgactat aaaaagaaag ggcttcccag gtggaagccc
     2521 tatttctttt atggaatcag caggctggaa ccttgcgtcg cccggctttc cagaatctca
     2581 tgcgcacgct gcgcatcctt cagcggatat ttctgctgct cggcgacatc gaccttaatc
     2641 acaccgctgg caatcaaaga gaacagttca ttactggcct cggttaattc ctcccgtgtg
     2701 gtgatatagc cttgcaggga agggcgtgtc acatacaacg agcctttttg attgagaatg
     2761 cctaagttca caccggtaac cgcacctgat gagttgccaa aactgaccat taagccgcgg
     2821 cgttgcaggc aatccagcga ccgttcccag gtgtctctgc ccacggaatc gtacactacg
     2881 cgcactttct taccgccggt gatctctttc aatcgctcga ccagattctc ttcacgatag
     2941 ttaataacct gccacgcgcc ggcttttagc gcgctctgcg ctttttgcgc ggttcctacg
     3001 gtgccgataa gtttcgcgcc cagagctttt gcccactggc aggcaatcaa gccaacgccg
     3061 ccagccgctg cgtggaacag gaactgttca tcgggtttaa tttcataggt tttgcgcagc
     3121 agataataaa ccgttaagcc tttcaggaaa gatgctgcag cttgctcaaa agaaatagcc
     3181 gcaggcagaa tcgccgcttt atccgcatta atgttatgca cagagctgta agcgcctaac
     3241 gccgactgcg catagactac acgatcgcct gccttaatat gctttacacc actgccgact
     3301 ttactcacga tgcctgccgc ttcggtacct gatccgctgg gtagtgatgg cggcgggtaa
     3361 aggccgctgc ggatatacgt gtcgataaaa ttgatgccga tggctttatt ttcgacctgg
     3421 atttcattct ctgccggatc ggcaggagtg aactctacgg cttgaagtac ttccgggcca
     3481 ccgtgcttgt gaaattcaat tcgtgttgcc atgtgtcctc cagaacgtat cgtcagggtc
     3541 tgcttcatat ggtaaagttt cgacccattc tttatctcgg taactccatt cactatggca
     3601 ggaaataaac ccttcaacaa acagcaggct gaaccccgcg aacgcgatcc acaagttgcc
     3661 gggctgaaag tgcctccgca ctcgatcgaa gcggagcagt cggtgttggg cggtttaatg
     3721 ctggataacg aacgctggga cgatgtagcc gagcgtgtgg tggcagacga tttttacacc
     3781 cgcccacacc gccatatctt tactgaaatg gcgcgtttgc aggaaagcgg tagtcctatc
     3841 gatctgatta cccttgcgga atcgctggaa cgccaggggc aactcgatag cgtcggtggt
     3901 tttgcttatc tggcagagct gtcaaaaaat acgccaagtg cggcgaacat cagtgcttat
     3961 gctgacatcg tgcgtgaacg tgccgttgtc cgtgagatga tctcggttgc gaatgagatt
     4021 gctgaagccg gttttgatcc gcaggggcgt accagcgaag atctgctgga ccttgctgaa
     4081 tcccgcgtct ttaaaattgc cgaaagtcgt gcaaacaaag acgaagggcc gaagaacatc
     4141 gccgatgtgc tcgacgcaac cgtggcgcgt attgagcagt tgtttcagca gccacacgat
     4201 ggcgttaccg gagtaaacac cggttatgac gatctcaaca aaaaaaccgc tggcttgcag
     4261 ccgtcggatt tgatcatcgt cgccgcgcgt ccgtcgatgg gtaaaacaac atttgcgatg
     4321 aacctcgtcg aaaacgcggc gatgttgcag gataaaccag tacttatctt ctcgctggag
     4381 atgccttcag aacagattat gatgcgttct ctggcgtcgc tgtcgcgcgt tgaccagact
     4441 aaaatccgta ccgggcagct cgatgatgaa gactgggcgc gcatttccgg caccatgggt
     4501 attttgctcg aaaaacgcaa tatctatatc gatgactcct ccggcctgac gccaacggaa
     4561 gtgcgttccc gcgcacgacg tattgcccgt gaacacggcg gcatcgggct tatcatgatc
     4621 gactacctgc aactgatgcg cgtaccggcg ctttccgata accgtacgct ggaaattgca
     4681 gaaatctctc gctcgctgaa agcactggcg aaagaactga acgtgccggt ggtggcgctg
     4741 tcccagttga accgttctct ggaacaacgt gccgacaaac gcccggtcaa ctccgacctg
     4801 cgtgaatctg gctctatcga gcaggatgcg gacttgatca tgtttatcta tcgtgatgag
     4861 gtgtatcacg aaaacagtga tttaaaaggc atcgcggaaa ttattatcgg taaacaacgt
     4921 aacggcccaa tcgggacggt acgcctgacc tttaacggtc aatggtcgcg cttcgacaac
     4981 tatgcggggc cgcagtacga cgacgaataa taattatttt atgaattagg caattaaagc
     5041 aaacacttat caaggaacac aaatgcaagc ggcaactgtt gtgattaacc gccgcgctct
     5101 gcgacacaac ctgcaacgtc ttcgtgaact ggcccctgcc agtaaaatgg ttgcggtggt
     5161 gaaagcgaac gcttatggtc acggtcttct tgagaccgcg cgaacgctcc ccgatgctga
     5221 cgcctttggc gtagcccgtc tcgaagaagc tctgcgactg cgtgcggggg gaatcaccaa
     5281 acctgtactg ttactcgaag gcttttttga tgccagagat ctgccgacga tttctgcgca
     5341 acattttcat accgccgtgc ataacgaaga acagctggct gcgctggaag aggctagcct
     5401 ggacgagccg gttaccgtct ggatgaaact cgataccggt atgcaccgtc tgggcgtaag
     5461 gccggaacag gctggggcgt tttatcatcg cctgacacag tgcaaaaacg ttcgtcagcc
     5521 ggtgaatatc gtcagccatt ttgcgcgcgc ggatgaacca aaatgtggcg caaccgagaa
     5581 acaactcgct atctttaata ccttttgcga aggcaaacct ggtcaacgtt ccattgccgc
     5641 gtcgggtggc attctgctgt ggccacaatc gcattttgac tgggtgcgcc cgggcatcat
     5701 tctttatggc gtctcgccgc tggaagatcg ctccaccggt gccgattttg gctgtcagcc
     5761 agtgatgtca ctaacctcca gcctgattgc cgtgcgtgag cataaagccg gagagcctgt
     5821 tggttatggt ggaacctggg taagcgaacg tgatacccgt cttggcgtag tcgcgatggg
     5881 ctatggcgat ggttatccgc gcgccgctcc atccggtacg ccagtgctgg tgaacggtcg
     5941 cgaagtaccg attgtcgggc gcgtggcgat ggatatgatc tgcgtagact taggtccaca
     6001 ggcgcaggac aaagccgggg atccggtcat tttatggggc gaaggtttgc ccgtagaacg
     6061 tatcgctgaa atgacaaaag tgagcgctta cgaacttatt acgcgcctga cttcaagggt
     6121 cgcgatgaaa tacgtggatt aatcgttctg taatatttga ttgtctgtgc cggatgcggc
     6181 gtgaatgcct tatccggcca ataaaatcct aaaaattcaa taagttgatg ttctttcatg
     6241 ctcttataaa ggtcgtgcct ctggcggatg ttcgtttgtc atgagtctca ctctgttgct
     6301 aattgccgtt cgctcctgaa catccactcg atcttcgcct tcttccggtt tattgtgttt
     6361 taaccacctg cccgtaaacc tggagaacca tcgcgtgttt caaaaagttg acgcctacgc
     6421 tggcgacccg attcttacgc ttatggagcg ttttaaagaa gaccctcgca gcgacaaagt
     6481 gaatttaagt atcggtctgt actacaacga agacggaatt attccacaac tgaaagccgt
     6541 ggcggaggcg gaagcgcgcc tgaatgcgca gccccatggc gcttcgcttt atttaccgat
     6601 ggaagggctt aacagctatc gccatgccat tgcgccgctg ctgtttggtg cggaccatcc
     6661 ggtactgcaa caacagcgcg tagcaaccat tcaaaccctt ggcggctcag gggcattgaa
     6721 agtgggcgcg gatttcctga aacgctactt cccggaatca ggcgtctggg tcagcgatcc
     6781 tacctgggaa aaccacgtag caatattcgc cggggctgga ttcgaagtga gtacttaccc
     6841 ctggtatgac gaagcgacta acagcgtgcg ctttaatgac ctgttggcga cgctgaaaac
     6901 attacctgcc cgcagtattg tgttgctgca tccatgttgc cacaatccaa caggtgccga
     6961 tctcactaat gatcagtggg atgcggtgat tgaaattctc aaagcccgcg agcttatccc
     7021 attcctcgat attgcctatc aaggatttag tgccggtatg gaagaggatg cctacgctat
     7081 tcgcgccatt gccagcgctg gattacccgc tctggtgagc aattcgttct cgaaaatttt
     7141 ctccctttac ggcgagcgcg tcggcggact ttctgttctg tgtgaagatg ccgaagccgc
     7201 tggccgcgta ctggggcaat tgaaagcaac agttcgccgc aactactcca gcccgccgaa
     7261 tttgggtgcg caggtggtgg ctgcagtgct gaatgacgag gcattgaaag ccagctggct
     7321 ggcggaagta gaagagatgc gtactcgcat tctggcaatg cgtcaggaat tggtgaaggt
     7381 attaagcaca gagatgccag aacgcaattt cgattatctg cttaatcagc gcggcatgtt
     7441 cagttatacc ggtttaagtg ccgctcaggt tgaccgacta cgtgaagaat ttggtgtcta
     7501 tctcatcgcc agcggtcgca tgtgtgtcgc cgggttaaat acggcaaatg tacaacgtgt
     7561 ggcaaaggcg tttgctgcgg tgatgtaatg caggaaagca ggctggagtt acccagcctg
     7621 cagtgaaatt aaactgtcgt cgctttcact atttctttgt agatgatttt tttgatgccg
     7681 ttgttctacg tgagagataa taaaacgttg tcagttcttt tattgttaag tttatcccaa
     7741 ttaactggaa ttcctttccg gtaatgct
//
LOCUS       DAJTOR010000186         7644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTOR010000186 DAJTOR010000000
VERSION     DAJTOR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.186"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            564..3977
                     /locus_tag="OO249_003590"
     CDS             564..3977
                     /locus_tag="OO249_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5637746.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRN
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIAQIARLQKIVEELGVVTDAATV"
     gene            4041..5675
                     /locus_tag="OO249_003591"
     CDS             4041..5675
                     /locus_tag="OO249_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5637747.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5684..6754
                     /locus_tag="OO249_003592"
     CDS             5684..6754
                     /locus_tag="OO249_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5637748.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPPIYYQDEILDRLARIEKFKEK
                     IEISLNHLEIQFLSLQKRLIGF"
     gene            complement(6879..7595)
                     /locus_tag="OO249_003593"
     CDS             complement(6879..7595)
                     /locus_tag="OO249_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637749.1"
                     /translation="MLSETKKRIPAHLFRMIKDELYELVLTDDPKGKLYVRDSEKIEE
                     GVSTTEFVVGYGAISMVKKSESMATKGLVGLERVDLIREVVFENGYYDWGCVVNDVLP
                     NICKGNARIPIFHFLNHAKLINPDGSIINETELSGGVLSRLNITPISFQSQGWDKRRS
                     ETVPEVKVGVNELYLAYDFSFFLRMMPYMEPGLIKRDMGELLKILKKHIDEAMNTQVL
                     SSNFCKLVCIYDYIKNSHRY"
BASE COUNT         2222 a         1739 c         1793 g         1890 t
ORIGIN      
        1 tcctgttctc ccaaatatca tggtattgat acgggagatt atgccgtaca ggtacgacac
       61 gttatgtcgt gccaaagggg agtggataga aaaaatggcg aagatgcgaa aagttgatta
      121 atgcattgaa aaaaatgaat taataatgaa cttgggtaaa agcgccatca aaatacgaca
      181 cgacctgtcg tattttgtgt tagccgggaa aagatatctg ctacttacta aaatgattta
      241 gtgctgaaaa tatttataag ccaataaata gacaggtatt acggagtaac gcccaggcta
      301 ttttaacgag cctgctgttt tattggctca aaaaacatat cccatagatg atcagccata
      361 cccgcgttag tgagcaaggt atcccttgag tagccagggc tgaagacgcc cattgatggg
      421 cgaaaaccaa ggttctggcc gttaaagcgg ctatgtcgtt aaacacatca ccaccatatg
      481 ccatccggct cacttcgtcg gccaaattag tgtgatattc tttccagaat ttctgttgta
      541 tccacaacat aaggcgatga ttgatggagc agtctctcaa ttttgaaatg ttgcgcagcc
      601 agtggccgga actggcagaa ctcgcatgta tggcagagcg ctatgttcac tccgatccgg
      661 aaagctgcct ggttaagctg cgcaactaca ccgaattgat ggtgcgctgg ttgtatcgtc
      721 aggagcggtt gccggaaggt attaaggcta atctttacga tttaatgaac gctgatgtct
      781 ttaccagcat gatgccggaa gccatcatca tgaaaatgga tgcgctgcgt atccatggca
      841 accgtgccgc gcacggcgga cgtatcaaag ctaaagatac ttactggctg ctcaaagaag
      901 cgtatttgtt gggaatttgg ctgtatgttc gctacgccca cggtaatgtt gatgactgcc
      961 ccaaatttac actccctcca ttaacacaat cttcaggtcg tgcagatgaa aaacgtctgg
     1021 aagatgcaat caaggctcag gatgaaagcc gtgagcgcga actggcgcta caacgcgcac
     1081 tacagcaaga acaggaaaag gccgaacacc tcactcaacg cctgaatgaa gccagagcgc
     1141 gtaaccagca tgttgccgat attctctcta ttgatgaagc ggaaacccgc cgtcgcctga
     1201 ttgactctcg cttacttgca gccgactgga atgtagggga agaacttaaa aacacggatc
     1261 aagtcacaca agaacatcca gtcaaagagc aacccaccac ctctggtgac ggttatgcag
     1321 actatgtctt gtgggatgag gcacacaaac cgttggcggt ggtggaagca aaaaaaacca
     1381 gcgtcaatgc cgagcaagga cgaattcagg cccggctgta tgcagactgg ctggaaaaag
     1441 aatacggtca gcgtccggtc atcttctaca ccaacggcta tgatatctgg ctgtgggatg
     1501 accataaaac tcatggttat cccccgcgtc gggtgtttgg cttctacagc aaggaaagcc
     1561 tgcaatatct tattcagcag cgtgaaaccc gtctgccgct gaacagagtg ccacacgtaa
     1621 aagataacga aggtaaggcc gttgccggac gtttgtacca gctcgaaacc attgcccgcg
     1681 tcagtgaacg gtttaccaac aagtaccgac aatcgttaat tgttcaggct acgggtacag
     1741 gcaaaacccg cgtggcaatt gcactaagca aactgatgat tgatgcccgc tgggtaaaac
     1801 gcgtcctgtt tctttgcgac cgtaaagaac tgcgtaaaca ggcggcaaat gcctttaatc
     1861 aattcaccaa tgagccgctg tatgtggtcg ggaaatcgaa aaaagcagac aggcagaatg
     1921 ccagaatcta catcgctacc tatcccggta tgatgaaaat catggaccat ttcgatgtcg
     1981 gttatttcga tttgatcatc gccgatgaat cccaccgctc tatctacaac gtctacggcg
     2041 atctgtttaa gtattttgat gccctccaga ttggcctgac ggccacgcca attgacatgg
     2101 tgagtaaaac cactttcggc ctgtttggtt gtgaaggacg tattcctacc gccaactaca
     2161 gcctggaaga tgccatcgcc gataacaatc tggttcccta tgaggttgtc acacacacaa
     2221 cagaattcct gcgcgaaggt atcaaacggg agaaattaag cgacgcacaa atccgcgagc
     2281 tggaagaaca aggcatagat cccaatacgc tggagtttga tggcaaggcg ctggatgagg
     2341 cgatctacaa caaagacacc aaccgctata tcctgcgtaa cctgatggaa aatggcctga
     2401 aagatcggga cggccagttg cctggtaaaa ccatcatttt cgcccgtaac cacaaacacg
     2461 cgctgctgtt gaatgaattg ttcgacgata tgtacccaca gtttgccggg cgcttctgcc
     2521 aggtcatcga caactacgat ccccgcgccg agcaactgat tgatgatttt aaagggctgg
     2581 atgaaagcac caacaaagag ctgaccatcg ctatctccgt tgacatgctc gataccggta
     2641 ttgatgtccc ggaaattgtg aatctggtct ttgccaaacc ggtcaaatca aaagtgaagt
     2701 tctggcagat gattggccgt ggtacgcgcc tctgtccggg gttgtacggt tacgacgata
     2761 acggcaaacc gctggataaa cagaaattcc gcattttcga tcactggggc aattttgagt
     2821 atcacgagct gcataccgaa gaggcggaag tcacagcgac aaaatcgctt gcgcaaaaac
     2881 gcttcgaagc gtgggttatg ctgggggccg ctgcgcagcg taaattcgac aaacaggcgg
     2941 tggatttagt tgctcaccag cttcgcgagc aaatcaacgc actggatgaa aagtcgattg
     3001 ccgtacagga aaaatggcag cagaaagcgc agtacagtga tgaaaaggtg ctgcgccagc
     3061 tttctccgaa aacacagcag gatctgctat ctgttctggc cccgttgatg cagtggctgg
     3121 acgtgcgcgg gcaaagcgat gccatacgct ttgatatgga tattctggcg gcgcaaactg
     3181 cccgttatac caacccggaa gagctggatg ttctctggcc gatcatcgtc gaaaaagtgg
     3241 agcgcctgcc gccgcatttg gcgcaggtgc agcaacaggg acaacgaatc aatcagcttc
     3301 gtaatttaag ctggtggaag caggccagcc tggaagagct ggaggatatt cgcattcatt
     3361 tgcgcggtat catgcacctg atggaaaaag acgcgacgcc taaatttggt tcgatacagg
     3421 tggatattac cgaagatgcg aacctgatcc agacggagac ccgcaaaact aatatccgct
     3481 cgattgattt caaactctat cgccagcagg ttcagggggc gctggagccg ctgttccagc
     3541 aaaacccggt tctgaagaaa atccgcaacg gcgagccagt cacgcaaaac gagctggatg
     3601 agctggcgaa gctggtgctg atccagaacc ccaacgttga tattcgtgcg ttgaaagagt
     3661 tttatccgca ggcaaccgcc agcctggata aacttttgcg taccatcatc gggatggaca
     3721 gcgacgcggt ggaagtgcgt tttgcccagt tcgccgctga taacagcctg accagccaac
     3781 aactgcgctt cctgtcattg ctgaaaaacc acattcgcga ttacggcacc attgaaatgc
     3841 ggcagctctt tgaacagcct tttacgcata tccacaacga aggcgttacc ggtgtgttcc
     3901 ctgatatagc gcaaatcgcc cgcctgcaaa agatagtcga agagctgggt gttgtgaccg
     3961 acgcagcgac ggtataatac acgcatgaca agccccggca gcggggcttt tgtatttaaa
     4021 acaggatata aacgctaaca atgattactg gcgacttaaa aagtaaaatc gacggactat
     4081 gggaagattt ctgggtgggt ggtatcacca acccgctgac cgtaatcgaa cagatcacct
     4141 atctgatgta ttcccggatg ctggataccc aggaacaacg cgacgaaaag cgtaaacaaa
     4201 tcgcgggtat tgattttaaa ccgcgttttg cgccagaaca gcaggagttc cgtttcagtc
     4261 actatagcaa ccttggctcg gatgagatga tggaagtggt gcgcgatggc gtattccagc
     4321 atttccgtca gctcggccag gccgatgctt cgaaggtgac gctgctgggc aactttatga
     4381 aagatgctcg tctggagatt gttaagccgt cattgctgac caaagcggtt gaggtaatca
     4441 aaaacctgcc attggatcgc ggcgacacca aaggcgacct ttacgaatac ctgttaagca
     4501 agctgacaac tgccggaatc aacggacagt tccgcacacc gcgccacatt atccgcacga
     4561 tggttgaaat gatggagccg aacccggccc gcggcgagac gatttgcgat cccgcctgtg
     4621 gcaccggtgg ttttctggca accagctatg aatatctgct ggagaagtac agctcgctgg
     4681 aatccattca tactgagatt ggcaccaacg aacgtggcga gctggaagag caaaaaatct
     4741 ttaccggcga tctgctgaca ccgtggcgta accatgtgga taacaacatg ttccacggtt
     4801 acgactttga caccacaatg ctacgtatcg ccgccatgaa cctgattatg cacggcgtgg
     4861 atgcgcctga tatccactat caggacacaa tgagccagag tttcagcaca aacttcccgc
     4921 aggccagtaa aaacgccttc aacctgattc tggcgaaccc gccgtttacc ggttcactgg
     4981 acgaggaaga tatcgactcc acgctgtcgg caatggtgaa aaccaaaaaa accgaactac
     5041 tgttcctggc gcgtattctg caaatgctga aagtgggcgg gcgcagtgcc actatcgtgc
     5101 cgcagggcgt gctgtttggc tctagcaagg cgcaccagtc acttcgcaaa acgctggtgg
     5161 aagataacca actggaaacg gtgatcaatc tgccttctgg tgtatttaaa ccttacgctg
     5221 gcgtggcgac ggcgatcctg atctttacca aaggcggtca aacggatgag gtctggttct
     5281 acgatctaca aaatgacggc tacagcctgg atgataagcg caacccgata aaagacaacg
     5341 atctgccgca tctgctggca agctggaagc attaccgtac tttacgcggg ctaccggttg
     5401 ataactttat gggtaagaag ttagcctcgt tgcttaaaca gcagtacccg gaagggatta
     5461 atgctggcgt tgattttaaa gatcgcacgc aggcggcgtt tgttgtaccg aaagcggata
     5521 ttgctgcgca gaaatacgat ctatccatca accgttataa agaagtcgtg tatcaggcgg
     5581 aggaatatga agatccgaag gtgatattga agcggttaaa ggatctggaa aaagagattc
     5641 tggcggattt ggatgagctg gaggggatgc tgtgagttcg gcgatggtga agttagggga
     5701 tgttattaat gttcactacg gaaaagcatt aaaagctgat cagcgtgtat cgaatggaag
     5761 tgtccatgtg tttggtagtt caggaattgt cggcaaccac gataaaacgc tttgcagcta
     5821 tcccacgata attattggcc gcaaaggcag cgttggtgct attacctggg ctccaagcgg
     5881 aggctggatc attgatactg cttattacgt tgagatcaaa gataataata agttagatct
     5941 ccgatacttg ttttatattc tgagcggaat tgatctcact aagaaaacga ttaccacaag
     6001 tattcctgga ttaaatagag acgatctcta tgatacattt attaaattac caccttttga
     6061 agagcagaaa cgaattgtgg atcttctcga taaagctgag ggtatacgcc aaaaacgtga
     6121 gcaatccatc aaactggctg atgatttttt gcgtgctacg tttgctacaa tgtatgggaa
     6181 tcctattaca aaccccaaaa aatggcctgt ccatcttatg ggagaaatta ttgaatttaa
     6241 aggtggaaat caaccaccta aaagtgattt tatttttgaa cctaaacagg gttatattag
     6301 attagtacaa attagagatt ttaaatccga taaatatgcg acgtacattc cgcaagaaaa
     6361 agcaaaacgt attttcgaag ttgatgatgt tatgattgct cgttatgggc cacctgtgtt
     6421 tcaaatactt agaggtttat ccggcagcta caatgtagct ttaatgaaag cttcacccaa
     6481 agaaaatatt aggaaggggt ttatattcta tcttctacaa cttccggaat atcatgatgt
     6541 tgttgtaaaa aactctgaaa gaacagcagg acaaaccggt gtaaatcttg aactattaaa
     6601 caaattcaat gttcccctcc cacctatata ctatcaagat gagatattag atagattagc
     6661 tagaattgaa aaatttaaag aaaaaataga aatatcatta aatcatttag aaatacaatt
     6721 tttatcacta cagaagagat taataggttt ttaaatcctt agtatcgaaa taaacattaa
     6781 tttattatat taaattaaga tattttagga aattaaggcg tcactcttag gtaacgcctt
     6841 tttttactat ctcgccccct aagaaggtta tatcttactt aatacctgtg actatttttg
     6901 atataatcat aaatacacac taatttgcaa aaattacttg acaatacctg agtattcata
     6961 gcttcatcta tatgtttttt caatattttg agaagctctc ccatatcgcg cttgattaat
     7021 ccaggctcca tataaggcat cattcttaaa aagaaactga aatcataagc aagatataac
     7081 tcattgaccc ccaccttgac ctcaggaacg gtttcagagc gtcgtttatc ccatccttga
     7141 gactgaaaac taataggagt gatatttaat cgtgataaaa cgccacctga gagttctgtt
     7201 tcattaatta tactcccatc aggattaatt aatttagcgt gatttaagaa gtgaaaaata
     7261 ggaatgcgcg cattgccttt gcatatattt ggcaaaacat cattaacaac acatccccaa
     7321 tcataatatc cattttcaaa tacaacctct cggatgagat ctacacgctc cagtccaacc
     7381 aatcctttag ttgccatcga ttccgatttt tttaccatcg aaatagcacc atatcccaca
     7441 acaaactcag ttgtactgac cccttcttca attttctctg aatccctcac atataatttt
     7501 cctttcggat catcagttaa gaccaactca taaagttcat cttttatcat tcggaataaa
     7561 tgtgctggaa tccttttttt agtttcactc agcacctcaa atatttcttt ataggatggt
     7621 gcctgaacat attttaccgg gatt
//
LOCUS       DAJTOR010000187         7474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTOR010000187 DAJTOR010000000
VERSION     DAJTOR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.187"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..341
                     /locus_tag="OO249_003594"
     CDS             <1..341
                     /locus_tag="OO249_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5637750.1"
                     /translation="QTEAFLNAYGLSRFAPLGYDPRDLPIRDLAGYRKKGNHDGDPII
                     FYTFPAAFEQEIAKGFNTKQFAEVLKNAGMLTPPTSGRGYQGRVREDGRQIRVYVLNF
                     MAEESSQPEE"
     gene            716..1150
                     /locus_tag="OO249_003595"
     CDS             716..1150
                     /locus_tag="OO249_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR5637751.1"
                     /translation="MTAQISAYGRLVADPQTRTTTNGNNMAMARLAVSLPCNAAEAGE
                     STFWLGVIAFGKQADALAKHQKGDLVSVAGNMQLNQWTGQDGGTQQGYQVVADSVISA
                     RTVRPGGKAGQRGQATDALRRAQQPSRDEYDQRPPFDDETPF"
     gene            1161..1802
                     /locus_tag="OO249_003596"
     CDS             1161..1802
                     /locus_tag="OO249_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637752.1"
                     /translation="MSTQPIIIEFGSYRWPRHLAIHEADHAIAAWFFGIQEVEIALSG
                     NNRVAKTYLSGDVDECGAVAVWHANYPRNVAEVKSYTLSNDDTRLRLIDQVCRAMVIS
                     LAGIVAQSRYTGDDINALMETSGKADMKNVRDLSEVYRVAGGEDADIQRISLSRAEAL
                     IALKWWDVIALAKILENRNHMPASNFQRIMGDIDKPVPKPMTLAELDELVGEE"
     gene            1799..2149
                     /locus_tag="OO249_003597"
     CDS             1799..2149
                     /locus_tag="OO249_003597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637753.1"
                     /translation="MKPYILAAGLISSAPALSAIHCGGFTIQPDRYRTTINGDVVHIV
                     DAQFYGAPRDFSQAAIKLLPDAITTTDRQYRIETQGGNALLELMTRENPPRVLNRERC
                     DAGLTSFGWYTSAP"
     gene            2843..3454
                     /locus_tag="OO249_003598"
     CDS             2843..3454
                     /locus_tag="OO249_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637754.1"
                     /translation="MRDRTRSYTTDLPRIGLPFLANMRSRLADAEPGTQLYTQTESGT
                     LYAFRQADSYAMTINGVTRAIRTTTTKAGYGVREWYACPHCMKRAAKLYIGKKDIGCR
                     ACWKLHYKSQSADILDRMRMKIRQQRCAIWGSNDLANNLFNDIRMFPKPKGMRWATFD
                     RKRAELSVMEMAYWQAFSPVVDRITGVIERKAGKAARGIGLTL"
     gene            3992..4534
                     /locus_tag="OO249_003599"
     CDS             3992..4534
                     /locus_tag="OO249_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCR5637755.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSSINDRLQTLN"
     gene            complement(4527..4886)
                     /locus_tag="OO249_003600"
     CDS             complement(4527..4886)
                     /locus_tag="OO249_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637756.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            5320..6144
                     /gene="dinD"
                     /locus_tag="OO249_003601"
     CDS             5320..6144
                     /gene="dinD"
                     /locus_tag="OO249_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5637757.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            6436..7053
                     /locus_tag="OO249_003602"
     CDS             6436..7053
                     /locus_tag="OO249_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5637758.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7050..7474)
                     /gene="ligB"
                     /locus_tag="OO249_003603"
                     /gene_synonym="yicF"
                     /pseudo
     CDS             complement(7050..7474)
                     /gene="ligB"
                     /locus_tag="OO249_003603"
                     /gene_synonym="yicF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+;
                     internal stop; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
BASE COUNT         2001 a         1834 c         1908 g         1731 t
ORIGIN      
        1 agcagacgga ggcgtttttg aatgcctatg ggctgagtcg attcgcgccg ctgggttatg
       61 acccgcgcga tttacccatc cgtgatttag ccgggtacag gaagaagggg aaccacgacg
      121 gcgacccgat aattttctac accttcccgg cagcgtttga gcaggagatt gcaaagggct
      181 tcaataccaa acagtttgcc gaagttctaa agaatgcagg aatgcttaca ccaccaacga
      241 gcgggcgagg ctatcagggg cgagtaaggg aggatgggcg acagattcgc gtctatgtac
      301 tcaatttcat ggcagaggaa agcagccagc cagaagaata attatttctt cacatgcgta
      361 gttttagtgt tgtctcggtt gtctttgttg tctcaatttg taattaagat tgatttataa
      421 ggattttatt ttaatttctg agacaacacc gagacaacat ttagccattt tgagacaaca
      481 gggcgaaagt gtgtttttag tctggcaggc agtgagacaa catttatgcc gggtgagaca
      541 acacgaaatt gcaaatgttg tctcagcaaa acccagacgt ggcgcggcct gtagagccgt
      601 gagacaacaa agacaacaga gacaacacta tttttgctta ctatgaaaaa aaatcggcag
      661 gtgtcccaca ttaagaaatc gtcattagca aaactaataa tccagtgagg ggaaaatgac
      721 agcacaaatt tcagcatacg ggcggctggt ggcagatccc cagacccgca ccacaacgaa
      781 cggtaacaac atggctatgg cgcggctggc ggtgtctctc ccctgtaatg cggcagaggc
      841 gggagagtca accttctggc tgggcgttat tgcttttggg aagcaggccg atgcgctggc
      901 aaagcaccag aaaggcgacc ttgtgagcgt ggcaggcaat atgcaactca accagtggac
      961 agggcaggac ggcggtacac agcaggggta tcaggtagta gccgacagtg tgatcagcgc
     1021 cagaacggta cgccccggcg ggaaggcagg acagcgggga caggcaacgg acgcgctgag
     1081 acgtgcgcag cagccatccc gtgatgaata cgaccaacgc ccaccattcg acgatgaaac
     1141 gccattctga gagggaagat atgagtacgc aacccattat tattgagttc ggcagctatc
     1201 gctggcccag acatcttgca atacatgaag cagatcatgc gatagccgca tggtttttcg
     1261 gcatacagga ggtagaaatt gcgttatcgg gtaataaccg agtggcaaaa acctacctta
     1321 gcggcgatgt tgatgaatgt ggagctgtcg cggtatggca cgccaactat ccacggaatg
     1381 tggctgaggt taagtcttac accctctcga atgatgacac caggttacgt ctgatagacc
     1441 aggtatgccg ggctatggtt atctctctgg ctggtatcgt cgcgcaatca cgctatacgg
     1501 gggatgatat taacgcactg atggaaacgt ccgggaaagc agacatgaaa aatgttcgtg
     1561 atttatcaga ggtataccgg gtggctggtg gggaggatgc tgatatacag agaatatccc
     1621 tttcccgtgc tgaggcgctg attgccctga aatggtggga tgttatcgcg ctggcgaaaa
     1681 tactggagaa ccgtaaccat atgcctgcca gcaacttcca gcgcattatg ggtgatattg
     1741 acaagcctgt accaaagcct atgacgctgg ctgaactgga tgaattggtg ggtgaagaat
     1801 gaagccatac attctggcag ccggcctgat ctcctcggca ccggcactat ctgctatcca
     1861 ttgtgggggc tttaccatac agccagaccg ttatcggaca acgattaacg gcgatgtggt
     1921 tcatattgtt gatgcgcaat tttacggcgc accgcgagac tttagccagg ccgccataaa
     1981 gttattgccg gatgccatta ccactaccga taggcaatac cgcatagaga cgcagggagg
     2041 caatgccttg cttgagttga tgaccagaga aaacccgcca cgtgtgctta atcgtgagcg
     2101 ctgcgatgcc gggttaacaa gtttcggttg gtacacttct gcgccgtgat gttcgttccg
     2161 agcgcagcct aaaatattgg actgttcttg acggatttaa aactctaatg gagccaagtc
     2221 cttgccagta gcgggctagg agcctaattg tgattcaggt cgtcaaaaat attcaggttt
     2281 gcggcattgg ttcgcagtac gcagtccggt acgcggcaaa actgcgcatt taacgtgcgt
     2341 accacctgac gaattctaac gccggcattg caaacagatg cgtgcgcaaa atggctaaga
     2401 cgtctgatat tgctggcttt ggctcaatca gtttggaggt ggcggtatca gtacgatgct
     2461 aatttttgct aaggttttag tacgcaactc aggtacgcac tctcaccaga tacgaaaccg
     2521 attcgcagtt gcgtaccagc ctgtaaggcg cattatcgct gggtttctac agtcgaatgc
     2581 agtacgcaaa gtggaaaatc ctccgaatac gaaacccgag cattgcgcag taaattgcga
     2641 attgcgcagt taacaaaata tctccgcaaa acgaaacgga tatcaatgcg caaaccatcc
     2701 tcaacccccg cgcggttact ggctttattg atgtgaaatt gcaaaattgg tgcgcaattt
     2761 acagtaaaat acgcatatta tttcgtattc aatttgcgca ccgttcgcag ttaatgcgca
     2821 gtaattcgca ggaggccagc cagtgagaga cagaacgcgc agctatacca ccgacctgcc
     2881 ccgtataggg ttgccattcc ttgcgaacat gcgtagcagg ctggcggatg ctgaaccggg
     2941 tacgcagctt tatacccaga ccgaaagcgg aacgttgtat gccttccggc aagccgacag
     3001 ctacgccatg acaatcaacg gcgtgacgcg ggctattcgc accacaacca cgaaggccgg
     3061 gtatggcgtc cgggaatggt acgcttgccc ccactgtatg aagcgggctg caaagctgta
     3121 tatcggcaag aaagacattg gctgccgggc gtgctggaaa cttcactata aaagccagag
     3181 tgccgacata ctggacagga tgcgcatgaa gatacgccag cagcgatgcg cgatatgggg
     3241 cagcaatgac cttgccaata atctgtttaa tgacattcgc atgttcccaa aaccaaaggg
     3301 gatgcgctgg gctacgtttg acaggaagcg ggcagagtta tcagttatgg agatggctta
     3361 ctggcaggca ttcagcccgg tagtggacag aatcacgggc gttattgagc gtaaagcagg
     3421 aaaagcagcc agaggcattg gcctgactct ataaaagcaa aacgcccgca cggggcgtca
     3481 gagaacggga tagacttctg cgacaatttg cattcgccgc caatatagca agccgtccac
     3541 atcaagcaat aaactatcac cgggaggggg aggccaaatc tctgcaatcc tgagccttaa
     3601 ggactgcccg ccccatcgtt ttttcatacc cgcgaaaaat gaaatttaat ccgggcgtgt
     3661 ttcatccttc cagagggtat gacgtatgac accacgcgaa atagccttat tgaccatcgc
     3721 caaacttgag catggaggcc accagcttac acaggcagat caacgggaga tagaacgctc
     3781 agttaatgcc gatatagccc ggcgcgacag gttccgcgaa atgatgcgag cacctgccta
     3841 ccagaggaag aagccagcgc cgcgcaggta gatacgggat tattccgcat gtaacttatg
     3901 cgtattaagc aaatttcctt tattcacctt tacagaattg tgagtacgtc agtatcgtta
     3961 tccttccgat tcgcatttat tcaggatttg catgtaccag ctaaagataa ccatccgaga
     4021 cagcaaacca cctatatggc ggcgtgtgct ggttccagag caaatcccct ttagtgaact
     4081 tcacgcggtg atccagttgg cttttggctg gaatgacgaa cacctgtata tgttcgagaa
     4141 agggcgtaaa ggtgatcccg gtagcgagta tcgcgtatgg ggtgaggatg aaagcatggg
     4201 taatgcggca atcacaccac tatgggcagc gctccagaat gatggtgaca agctggttta
     4261 cacgtatgac tttggcgact ggtgggattg cgtcattgtg ctggagaagc aaacacacga
     4321 tacgagcaat cagcccatta gctgcctgcg tgggaaaggc accaccccgg tggaaaactc
     4381 aggcggcttg catggttaca atgaattgct attacaggcc agagagtctg ataatcctga
     4441 gcaggccgag atccacaatt tcctgatgct ggatattgag cgccgggttt acgacctgag
     4501 cagcatcaac gacagattgc agactcttaa ctgacccgca tatattcgcc tgatattgcg
     4561 ttatcagcgc cgccagtatc gcgcataaca gcgcactgac cgggcaaacc gtagcgcacg
     4621 accgttgcgt tagtctcacc gccgcgccgt atctggcgcg attcatacac tgtcacccgc
     4681 tcaagcagtc caccagcaac catgctttcc agtgtgcgcc gggttgattc gagctggtga
     4741 cgcttatcga acgacaccat gccatgaagc aggtaggcca cacccgacac atcgaacggc
     4801 ggcgcaccaa tctcaccagt cacccattcg aggttatccg gttcaaagta gctaagtatc
     4861 tcttttttgc ggctggtcat tctcatagct ggcaggttcc ttattgtggg atagcactat
     4921 catacaataa ggcgcaaaag ataagaggcc agaacagcaa gcatgagagc ataaaacgag
     4981 cagccaaaga cagcgcctca aaataccgtg tacacaaggg tgtacctact gagaaaacgc
     5041 attacaagaa acacagcaac cacgggcgtt taaagccact actcaataaa cagattcaac
     5101 tcgtaacgcc aattcttact tttcagcctt cacaaatgcc gcccactcaa acagagcggc
     5161 atttttcttc cccgcaactt caattctgtt ttgcgtgcct gctccagatt ttgcgatgtt
     5221 tttttgccca gcacactgag aacgtgagat actcacaact gtatataaat acagttacag
     5281 atttactttc tttgcaatcg atatcacatg gagtgggcaa tgaacgaaca tcatcaacct
     5341 tttgaagaaa tacggcatta tggcactgaa gggcaagaat tttggtcagc tagagaattg
     5401 gcaccgttac tggattatcg ggactggcgc aattttcaaa aggtactggc ccgcgcgact
     5461 caagcgtgcg aagccagcaa tcaagctgca tctgaccatt tcgttgaaac caccaaaatg
     5521 gttgtattag gttcaggggc tcaacgagaa cttgaagatg ttcatctctc ccgctatgcc
     5581 tgttacctgg tagtacaaaa cggcgaccct gcgaaaccgg ttattgcggc agggcaaact
     5641 tattttgcta tccagacccg acggcaggag ctggctgacg atgaagcttt caggcaactt
     5701 cgtgaagacg aaaagcgtct ttttctgaga aatgaattga aagaacataa caaacaattg
     5761 gttgaggcag cgcagcaagc tggggtagcg acagctatcg atttcgccat atttcagaat
     5821 catggttacc gggggctgta tggtggatta gatcagaaag ctattcatca gcggaagggg
     5881 ctgaaaaaga atcagaatat cctggatcat atgggttcaa cagaactggc ggctaatctc
     5941 tttcgagcta cccaaacaga agaaaaactc aagcgggatg gcgtgaattc aaaacagcaa
     6001 gcaaacacca cacactttga cgtgggtcgc aaggtgaggc aaaccattca ggaacttggc
     6061 ggaaccatgc ctgaggagtt gccgaccccg caggtcagca tcaagcagtt ggaaaacagt
     6121 gtaaaaatta cagagaagaa atagcagaaa ctaaaaactc tttttcttaa ttgagatacc
     6181 cgatgcgtaa gccaaagtcc aggtgcagtg aacacaatgg ctaaacattg cacctttctt
     6241 ttccacccct caattttaac ctattttttc ttatgcattt tctcagacaa gaactcagaa
     6301 taatgcatct acgctacaga aaatagcgat ttcacataac tacaagttat caatttcctc
     6361 ttaccttaaa aaatctcaat cgtgacaatg cgcacaaatc gctaccctgc cagacagatt
     6421 tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcatcac tgccgaagcc
     6481 atgacggggg cgctggcggc cggacgacgg cgcatggata catttggcgt aattattatt
     6541 gcgaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg ccactatccg
     6601 ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc agtactcacc
     6661 actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct cgatgcgctc
     6721 ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg gcacggccca
     6781 attattgccg ttgtcgcggc ggtaaccacc ggcgtgtttg gcggcgtttt gcgcgatatg
     6841 ttctgtaaac gcatcccact ggtattccag aaagagttgt atgctggtgt ctcatttgcc
     6901 tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt ggtgattatc
     6961 tccaccctgg tattcggctt ttttgcccgc ttactggcgc tacgtcttaa gctgggatta
     7021 ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat ctgctgggca
     7081 gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat aacctgtctg
     7141 gcgcgcccag atccagtccc cggcagctgc tgtcagaact gctccgtgct aaataaaagt
     7201 tgcgactagg accgttcatc actggcatta agcgccgccc gggttagcgg tattcccatt
     7261 gccatcaccc agcgagtaaa aggctgctta cgagccagat taaactgatg ccataactgc
     7321 gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt taataaaagc
     7381 caagaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc ggcctcacca
     7441 atgccatcca gcccaagaac ctgttttgac ccca
//
LOCUS       DAJTOR010000188         7399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTOR010000188 DAJTOR010000000
VERSION     DAJTOR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.188"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..1459
                     /locus_tag="OO249_003604"
                     /pseudo
     CDS             1..1459
                     /locus_tag="OO249_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1470..2543
                     /locus_tag="OO249_003605"
     CDS             1470..2543
                     /locus_tag="OO249_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5637759.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVITHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2791..3831
                     /gene="pstS"
                     /locus_tag="OO249_003606"
     CDS             2791..3831
                     /gene="pstS"
                     /locus_tag="OO249_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5637760.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3918..4877
                     /gene="pstC"
                     /locus_tag="OO249_003607"
     CDS             3918..4877
                     /gene="pstC"
                     /locus_tag="OO249_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5637761.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4877..5767
                     /gene="pstA"
                     /locus_tag="OO249_003608"
     CDS             4877..5767
                     /gene="pstA"
                     /locus_tag="OO249_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5637762.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5858..6631
                     /gene="pstB"
                     /locus_tag="OO249_003609"
     CDS             5858..6631
                     /gene="pstB"
                     /locus_tag="OO249_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5637763.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            6646..7371
                     /gene="phoU"
                     /locus_tag="OO249_003610"
     CDS             6646..7371
                     /gene="phoU"
                     /locus_tag="OO249_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5637764.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1860 a         1719 c         1931 g         1889 t
ORIGIN      
        1 cagatgttga tgtcagcgtg ggtgaattgc gcgatgaaga cggatttaca ccggacgtcc
       61 ttcaggcgca aatacttcat ggtttttccc acgggatcac tttatatgga ggtatgcagg
      121 ctgctgaaaa ttatggttct gcagctctgg gtgtcggtaa agatcttggc gctttgggcg
      181 caatttcttt cgatgtgaca catgctcgtg cgaattttag ccatgatgat acagaaacgg
      241 gtcagtcata tcgctttctc tattcaaaac gatttgacga cacagacact agcttgcgcc
      301 tggttggcta tcgttactcc accgagggct actataccct caatgagtgg gcatcgcggc
      361 gcaacagccc tgaagacttt tgggaaacag gtaaccgacg tagtcgcgtg gagggaacgc
      421 taacgcagtc gttggggaga gattatggca atttatacct gacattaagc cggcaacaat
      481 actggcatac cgatgatgtc gaacgattaa tgcaatttgg ctacagcagt agctggaagc
      541 gtctctcgtg gaacgtctcc tggagttatt ccaatactgc cagacagggg acggggaaca
      601 accatgccag tgataacacc agtgagcaga tctacatgct ctctttatct gttcctttat
      661 cgggctggtg gggtaatagt tacgccacct attctgtttc gcaaaacgat aattccggta
      721 gctcacatca agcggtacgg cgctggaaag aaataacctt tcatggaatt taatgcagtc
      781 ctataacagt catgatgatg aggttggcgg taatatgtcc ctgacctatg atggctctta
      841 tggcacggtg aacggcagct ataactacag ccaaaattcc cagaggctga attatggtat
      901 cagaggggga attctggcac acagcgaagg ggtaacgtta agtcaggagt taggtgaaac
      961 tattgctctt gttaaagcac ctggggccgc cgggttagaa atagataata tgcgcggtgc
     1021 tgcgacggac tggcgcggct atacggtcaa gacacagcta aacccttatg atgaaaatcg
     1081 ggtagcaatc agcgataact atttctcgaa gtcgaatata gaacttgata ataccgtcgt
     1141 tacgatggtt cccacgcgtg gtgcagtggt taaagcggag tttgtgactc atgtgggtta
     1201 tcgcgttctc ttcagagtgt taaatgcaaa tggtaaaccg gtaccttttg gagccattgc
     1261 tgcgatacaa gatgcaagtt tggcagattc aggaattgtc ggtgaccgtg gcgaacttta
     1321 tctttctggt ctaccagaaa aaggacaggt tacgttatcc tggggagaaa acgcctcaac
     1381 aaaatgcatc ttcaattatt cactttcgac accagaaagt gagagcggat taattgaaca
     1441 gggtgtgaca tgtcattaaa aggattttta tgaacaagta tatcaaacag tggtgctttg
     1501 ctgtgtttat gctctcgtta agtagtgtag cacttgcagc tcctaaaggt atctgtaccc
     1561 ctgataatgg cgtatttcac agtacgcttg atttctcggg atatcttatt accgcgaacg
     1621 aaaataaggt tggaacgaca tttaacacca ctgtaactaa tggatcctca tatcctggac
     1681 gctgtcattg tgatactggt aacgtaggag aattccccta tatctattat acgtcaaaaa
     1741 taaaccaggc attaacttat gctggggttc actctaatat taattattat gatcttaatc
     1801 cgaaccttga tgttgggatt gctatagata ttcttggggt tgggtatgtg aatgcacctt
     1861 ttgaatatca tgctaacaac ccgtctggta atacaaagta caattgcaac cgcattgaac
     1921 ctttaagtat atctagtggg gctaaagcga tagtatattt ttatatcaag aagacatttg
     1981 caggaaaatt gattattcct gaaacaaaga tagtgacatt gtatggaaca attagccgtg
     2041 acaccccggt ggattactca cagccgatgg ctgatgttta tattcggggc gatattacag
     2101 ctccgcaaag ttgcgaaata aataatttac agccagttta ttttgatttt aaagaaatac
     2161 cagctgcaga tttttcatct gttgttggaa gtgcggtaat aacacataaa attactaaaa
     2221 cggtcactat tgagtgtgaa aacctgggga tactgaatac cgatgatatc agtacgtctt
     2281 tttatgctac cgaacccaat acggacaatt caatggtcgt aacttcaaac tctaacgtgg
     2341 ggataaaaat ttacgacaaa aataataagg aaatcaaagt gaacggtggt gagttgccaa
     2401 cagacatggg taaatcaaca gtgtatggtg aaaaatcagg tagtgtaact ttttcagctg
     2461 ctcctgcgag ccttactgga gctcgcccag ccccggggca atttactgca acagcgacga
     2521 taactgttga gattgtgcgc taataggtct gacaacgatt gttcatcaca tcgttgtcat
     2581 ctcacccatt tctttatcac ttttaaatgc atcatgacaa aaagatgaca tgtgggattt
     2641 tcatcttcac atttaaaatc agtaagttat ttggtaattc ctttctgtca taaaaatgtc
     2701 atattcctta catataactg tcacctgttt gtcctatttt gcttctcgta gccaacaaac
     2761 aatgctttat gaatcctccc aggagacatt atgaaagtta tgcgtaccac cgtcgcaact
     2821 gttgtcgccg cgaccttatc gatgagcgct ttctctgtgt ttgcagaagc aagcctgaca
     2881 ggtgcaggtg caaccttccc tgcgccggtg tatgccaaat gggctgacac ttaccagaaa
     2941 gaaaccggta ataaagttaa ctaccagggt atcggttctt ccggtggcgt aaaacagatt
     3001 atcgctaata ccgttgattt tggtgcctct gacgcgccgc tgtctgacga aaaactggct
     3061 caggaaggtc tgttccagtt cccgaccgtg attggcggcg tggtgctggc ggttaacatt
     3121 ccagggctga agtctggcga actggtgctg gatggtaaaa ccctcggcga catctacctg
     3181 ggcaaaatca agaagtggga tgatgaagcc atcgccaaac tgaatccggg tctgaaactg
     3241 ccttcacaaa acattgctgt agtacgccgc gcagatggct ccgggacttc cttcgtcttc
     3301 accagctacc tggcgaaagt gaacgaagag tggaaaaaca acgttggtac tggctctacc
     3361 gtaaaatggc cgatcggtct gggcggtaaa ggtaacgacg gtatcgccgc gttcgttcag
     3421 cgtctgccgg gtgcaattgg ttatgttgaa tatgcttacg cgaagcagaa caacctggcg
     3481 tacaccaaac tgatctccgc tgatggtaaa ccggttagtc cgaccgaaga aaacttcgct
     3541 aatgcagcaa aaggtgcaga ctggagcaaa accttcgctc aggatctgac caaccagaaa
     3601 ggcgaagatg catggcctat tacctctacc acgttcattc tgatccacaa agatcagaag
     3661 aaaccagaac aaggcacaga agtgctaaaa ttcttcgact gggcgtacaa aaccggggct
     3721 aaacaggcga acgacctgga ttacgccagc ctgccggata gtgtagttga acaggttcgc
     3781 gctgcgtgga agaccaatat taaagacagt agcggtaagc cgctgtacta ataaaactcc
     3841 aggccgggta cggtgtttta cgccgcatcc ggcattacaa aatgactttg taaacgcgtt
     3901 taactgaaga gtaacttatg gctgcaacca agcctgcttt taacccaccg ggtaaaaagg
     3961 gcgacataat tttcagcgtg ctggtaaaac tggcggcgct gattgtgcta ttgatgttgg
     4021 gtggcattat tgtctctctg atcatctcct cctggccgag cattcagaaa tttggtctgg
     4081 ctttcctatg gaccaaagag tgggatgcac cgaacgatat ctacggggcg ctggtgccga
     4141 tctacggtac gttggtgact tcgtttatcg cgctgctgat cgccgtcccg gtgagtttcg
     4201 gtatcgccct gttcctgact gagcttgcgc ctggctggct gaaacgcccg ctgggtatcg
     4261 ccattgagct gctggcagcc attccaagta tcgtttacgg catgtggggc ctgtttatct
     4321 ttgcgccgct gttcgccgtt tactttcagg agccggtcgg caatatcatg tcgaatatcc
     4381 cgattgttgg cgcgctgttc tctggccccg catttggtat cggtatcctc gcggcaggcg
     4441 tgatcctcgc catcatgatt attccgtaca ttgcggcggt aatgcgtgat gtgttcgaac
     4501 aaaccccggt gatgatgaaa gagtcggcct acggtattgg ctgcaccacc tgggaagtta
     4561 tctggcgtat cgttcttccg ttcaccaaaa atggtgttat cggcggcatc atgctggggc
     4621 tgggccgcgc gctcggtgaa accatggcgg tgacctttat catcggtaac acctaccagc
     4681 tcgacagcgc ctcgctgtac atgccgggca atagtatcac ctctgcgttg gcgaacgaat
     4741 ttgcggaagc ggaatccggt ctgcacgttg ccgcactgat ggaactgggc ctgatcctgt
     4801 ttgtgattac cttcatcgtc ctcgccgcat cgaagtttat gattatgcgc ctggctaaga
     4861 atgagggggc acgctaatgg ctatggttga aatccaaacc actgcggcgc tggctgaatc
     4921 tcgccgcaaa atgcaggcgc gtcgccgcct caaaaaccgt attgcgctga cgctctcgat
     4981 ggcgacgatg gccttcggcc tgttctggct gatctggatt ttaatgtcca ccatcactcg
     5041 cggtatcgac ggtatgtcgc tggcgctgtt cactgaaatg acgccgccgc ccaatacgga
     5101 aggtggtggt ctggcgaacg ccctggcggg tagcgggctg ttaattttgt gggccacggt
     5161 attcggtacg ccgctgggca ttatggcggg gatttatctg gcggaatatg gtcgtaaatc
     5221 ctggctggca gaagtgattc gcttcattaa cgacattctg ctctccgcgc cgtcgattgt
     5281 ggttggtctg tttgtttaca ccatcgtggt ggcgcagatg gagcacttct ctggctgggc
     5341 gggcgtgatt gccctggcgc tgttgcaggt gccgattgtt atccgcacca ccgagaacat
     5401 gctgaaactg gtgccggaca gcctgcgtga agcggcttat gcgctgggta caccgaagtg
     5461 gaagatgatc tctgcgatta cgctgaaagc gtcggtgtcc gggatcatga ccggtatcct
     5521 gctggcgatt gcccgtattg ctggtgaaac cgcgccgctg ctgtttaccg cgctctccaa
     5581 ccagttctgg agcacggaca tgatgcagcc gatcgccaac ctgccggtga cgatctttaa
     5641 gtttgcgatg agcccgtttg cggaatggca acaactggcc tgggccgggg tattgattat
     5701 taccctgtgc gtactgctgc tgaacattct agcgcgcgtt gtttttgcga agaataaaca
     5761 cggttaacac tttgccggat gcggcgtgaa cgccttatcc ggcctacggt aagcctgatt
     5821 agcgaagtgc atcaggcacg atgaggaaaa gattgcaatg agtatggttg aaactgcccc
     5881 gagtaaaatt caggttcgta atttgaactt ctactacggc aaattccatg ccctgaaaaa
     5941 catcaacctg gatatcgcta aaaaccaggt tacggcgttt atcgggccgt ccggctgcgg
     6001 taaatcgacg ctgctgcgta ccttcaacaa aatgtttgaa ctgtacccgg agcagcgtgc
     6061 ggaaggtgaa attctgcttg atggcgacaa catcctgacc aactctcagg atatcgcact
     6121 gctgcgtgcg aaagtgggca tggtgttcca gaaaccgacg ccgtttccga tgtccatcta
     6181 cgacaacatc gcttttggcg ttcgtctgtt tgagaagctc tcccgtgccg acatggacga
     6241 gcgcgtgcag tgggcattga ccaaagccgc attgtggaac gaaaccaaag ataaattgca
     6301 ccagagcggt tactctctct ctggtggtca gcaacagcgt ctgtgtattg cgcgtggtat
     6361 cgccattcgc ccggaagtgc tgctgctcga cgaaccgtgt tcggcgctcg accctatctc
     6421 taccgggcgt attgaagagc tgatcaccga actgaagcag gattacaccg tggtgatcgt
     6481 cacccacaac atgcagcagg ctgcgcgttg ttccgaccac acggcgttta tgtacctggg
     6541 cgaattgatt gagttcagca acacggacga tctgttcacc aagccagcga agaaacaaac
     6601 agaagactac atcaccggtc gttacggttg attcaggagt gcgttatgga cagtctcaat
     6661 cttaataaac atatttccgg ccagttcaac gccgaactgg aaagtatccg cacgcaggtg
     6721 atgaccatgg gcggcatggt ggagcagcag ctttctgatg caatcaccgc gatgcataac
     6781 caggacagcg atctggcgaa gcgcgtgatc gaaggcgaca agaacgtcaa catgatggaa
     6841 gtggcgatcg atgaagcctg cgtgcgcatt atcgccaaac gtcagccgac ggcgagcgac
     6901 ctgcgactgg ttatggtgat cagtaaaacc attgccgagc tggagcgtat tggcgacgtg
     6961 gcggacaaaa tctgccgtac tgcgctggag aaattctccc agcagcatca gccgttgctg
     7021 gtaagtctgg agtcgctggg ccgtcatacc atccagatgc tgcacgacgt gctggacgcg
     7081 ttcgcgcgga tggacattga cgaagcggta cgtatttatc gtgaagataa aaaagtcgat
     7141 caggaatacg aaggtattgt tcgtcaactg atgacctaca tgatggaaga ttcgcgtacc
     7201 attccgagcg tacttactgc gctgttctgc gcgcgttcta tcgaacgtat tggcgaccgc
     7261 tgccagaata tttgtgagtt tatcttctac tacgtgaagg ggcaggattt ccgtcatgta
     7321 ggcggtgatg agctggataa actgctggcg ggaaaagata gcgacaaata attcaccaga
     7381 caaatcccaa taacttaat
//
LOCUS       DAJTOR010000189         7183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTOR010000189 DAJTOR010000000
VERSION     DAJTOR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.189"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            167..274
                     /locus_tag="OO249_003611"
     CDS             167..274
                     /locus_tag="OO249_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637765.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(327..1085)
                     /locus_tag="OO249_003612"
     CDS             complement(327..1085)
                     /locus_tag="OO249_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5637766.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVTYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1372..2301
                     /gene="pfkB"
                     /locus_tag="OO249_003613"
     CDS             1372..2301
                     /gene="pfkB"
                     /locus_tag="OO249_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5637767.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2402..2692
                     /gene="ghoS"
                     /locus_tag="OO249_003614"
     CDS             2402..2692
                     /gene="ghoS"
                     /locus_tag="OO249_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5637768.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            2798..3658
                     /locus_tag="OO249_003615"
     CDS             2798..3658
                     /locus_tag="OO249_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5637769.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(3699..4235)
                     /locus_tag="OO249_003616"
     CDS             complement(3699..4235)
                     /locus_tag="OO249_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5637770.1"
                     /translation="MTYQQAGHIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4382..5050
                     /gene="hxpB"
                     /locus_tag="OO249_003617"
     CDS             4382..5050
                     /gene="hxpB"
                     /locus_tag="OO249_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5637771.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5213..5791
                     /locus_tag="OO249_003618"
     CDS             5213..5791
                     /locus_tag="OO249_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5637772.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKH"
     gene            5936..>7183
                     /gene="tcyP"
                     /locus_tag="OO249_003619"
     CDS             5936..>7183
                     /gene="tcyP"
                     /locus_tag="OO249_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR5637773.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLIS"
BASE COUNT         1804 a         1748 c         1772 g         1859 t
ORIGIN      
        1 attttcgata tatatgttga tgcataatat tattttttca taaaacgcag aaacagtgac
       61 caggtgtaca acgccaccca acggctgtaa taacaaaaaa tcccttgatg cccgtccctt
      121 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggaatgc cgactaaacg
      181 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
      241 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
      301 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
      361 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtaac gttgtaagtc
      421 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
      481 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
      541 catgcataag ccccggaggc atagcccagc ggctgagttt cactggcatt gtcggtatat
      601 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
      661 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
      721 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
      781 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
      841 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
      901 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
      961 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1021 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     1081 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     1141 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     1201 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     1261 atatatattt atctgcaaaa ttttaaataa agctccaata aatcatattg ttaatttctt
     1321 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     1381 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     1441 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     1501 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     1561 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     1621 gaagccaaag actggacccg gcagaattta catgtacatg tggaagcaag cggtgagcag
     1681 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     1741 caagttctgg aaattgaatc cggggccatc ctagtcataa gcggaagcct gccgccaggt
     1801 gtgaagctgg aaaaattaac ccaactgatt tccgcttcgc aaaaacaagg gatccgctgc
     1861 atcgtcgaca gttctggcga agcgttaagt gcagcactgg caattggtaa catcgagttg
     1921 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccggac
     1981 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     2041 tcccttggtc cacaaggagc gctgggtgtt gatagtgaaa actgcattca ggtggtgcca
     2101 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     2161 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     2221 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     2281 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     2341 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     2401 tatggccagt ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt
     2461 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     2521 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     2581 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     2641 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     2701 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     2761 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     2821 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     2881 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     2941 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3001 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     3061 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     3121 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     3181 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     3241 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     3301 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     3361 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     3421 tttcgaccct gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca
     3481 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     3541 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caaggttatt
     3601 tggcggtcag catttggtta ttgctcagca gtcactggat agattattag cagcatgata
     3661 tgggttgaag ataatggccg ctctgtgcgg ccttttgatt aaataaaccc aagcagagaa
     3721 aagaacacat aacctgcagc aatacaaata accggcagga tatacagtga gaaaaactgc
     3781 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     3841 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     3901 gcctggcgac tcattcgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     3961 aaaatcaccc agaacataac gttcacgcca ccattaaaat tgggcgtcgg ggagttatac
     4021 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag
     4081 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     4141 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     4201 aaaacagcaa tgtgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     4261 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     4321 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     4381 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     4441 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat
     4501 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     4561 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     4621 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     4681 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     4741 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     4801 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     4861 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     4921 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     4981 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     5041 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     5101 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     5161 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     5221 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     5281 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     5341 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     5401 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     5461 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     5521 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     5581 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctag ttccccaaaa
     5641 gggcaaccaa ctggaacgtt ttatctgtat ggcattattt gtctggtcgg tatggatgcc
     5701 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaataatca ataccttgca
     5761 gatccagttt catcggctta ttaagcatta ggttgaatac taaaaagaca aaaatcacct
     5821 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     5881 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     5941 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6001 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     6061 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     6121 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     6181 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     6241 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     6301 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     6361 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag
     6421 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     6481 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     6541 gaaactgctg aaggatgatg cgccgaaagg tgaacgcgtc ttagccgcta tcgataccct
     6601 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     6661 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     6721 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gcagtgcatg gcattctgct
     6781 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     6841 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     6901 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     6961 tcagaacggc tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt
     7021 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     7081 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     7141 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tcc
//
LOCUS       DAJTOR010000190         7138 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTOR010000190 DAJTOR010000000
VERSION     DAJTOR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.190"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..466)
                     /locus_tag="OO249_003620"
     CDS             complement(<1..466)
                     /locus_tag="OO249_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCR5637774.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFH"
     gene            complement(475..744)
                     /gene="fliQ"
                     /locus_tag="OO249_003621"
     CDS             complement(475..744)
                     /gene="fliQ"
                     /locus_tag="OO249_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5637775.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(754..1490)
                     /gene="fliP"
                     /locus_tag="OO249_003622"
                     /pseudo
     CDS             complement(754..1490)
                     /gene="fliP"
                     /locus_tag="OO249_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            complement(1490..1855)
                     /gene="fliO"
                     /locus_tag="OO249_003623"
     CDS             complement(1490..1855)
                     /gene="fliO"
                     /locus_tag="OO249_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5637776.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(1858..2271)
                     /gene="fliN"
                     /locus_tag="OO249_003624"
     CDS             complement(1858..2271)
                     /gene="fliN"
                     /locus_tag="OO249_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5637777.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMHRLSR"
     gene            complement(2268..3272)
                     /gene="fliM"
                     /locus_tag="OO249_003625"
     CDS             complement(2268..3272)
                     /gene="fliM"
                     /locus_tag="OO249_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5637778.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(3277..3741)
                     /gene="fliL"
                     /locus_tag="OO249_003626"
     CDS             complement(3277..3741)
                     /gene="fliL"
                     /locus_tag="OO249_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5637779.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALNTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(3846..4973)
                     /gene="fliK"
                     /locus_tag="OO249_003627"
     CDS             complement(3846..4973)
                     /gene="fliK"
                     /locus_tag="OO249_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5637780.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(4970..5413)
                     /gene="fliJ"
                     /locus_tag="OO249_003628"
     CDS             complement(4970..5413)
                     /gene="fliJ"
                     /locus_tag="OO249_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR5637781.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERLSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(5432..6800)
                     /gene="fliI"
                     /locus_tag="OO249_003629"
                     /pseudo
     CDS             complement(5432..6800)
                     /gene="fliI"
                     /locus_tag="OO249_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
     gene            complement(6800..>7138)
                     /gene="fliH"
                     /locus_tag="OO249_003630"
     CDS             complement(6800..>7138)
                     /gene="fliH"
                     /locus_tag="OO249_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5637782.1"
                     /translation="ASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
BASE COUNT         1606 a         1963 c         1939 g         1630 t
ORIGIN      
        1 tgtgaaaggt atcgaccagc agtgaaatca accataaatg accgttaaat gtcaggaaca
       61 gcagtaacgc cagcatatcc atgatacgcg ctaaaacggg catattgaga tggctggccg
      121 gatcgacaaa cgtcgcaaat gacagcccca tttgcagacc gataatttcg ccagcggttc
      181 gcacagcggc aaaggcaaat tgcatggtaa aaccaagcgc aatgccgatc aggatctgct
      241 gcacggccag ccacagagca aagaacgaaa aaacaggaac atcgttggca ggtaatgatg
      301 gggcaatgac gaacgtgatc atcattgcca gccccagttt tacccgtttc ggcacgctgc
      361 gttcgctcag aattggtgcg gtagaaatga gcgccagcac gcgcagtaac ggccagaagt
      421 acaggcttaa ccaggaaagc cattgatcgc ttgtcacctg catcattgta cggcctaccc
      481 gatgatatac ggcaggttag tgaacaaggt gcggacgtaa tccagcaaca gattgagcat
      541 ccacggtccg gcaataataa tggcaataaa tacggcgatg atttttggaa taaacgacag
      601 cgtcatttcg ttgatctgcg tggcggcctg caaaatactg atgataaggc ccgtgaccag
      661 cgccaccagc aacagcgggg cagccagtgc cagcgcgact ttcatcgctt cagtccccat
      721 catcatgacc gattcaggtg tcattttgcc tctctagctg taaaagctct gcgccagcga
      781 accgaccagc aattgccagc catccaccag tacaaacagc atcagtttaa agggcagagc
      841 aatggtggcg gggggaccat catcatcccc aatgccatca acacgctggc aatcaccagg
      901 tcgataatca aaaaaggaat gaaaatcgtg aagcctatct gaaatgcggt tttcaactcg
      961 ctggtcacgt aggccgggcg caaaatgcgc atcggcacgg cttcaggtcc ctgcaacggg
     1021 ccggtattcg ctagtctggc aaacaaccct aaatctgcct cacgggtctg acgcagcata
     1081 aactcacgta gcggctgcgc ccctttttcc agcgcctcct gcattgatat tttctcttcg
     1141 ctgaatggct ggtacgcatc gacataaatt ttgtcgatca ccggtgacat aataaaaaag
     1201 gtcaaaaata gtgccagccc cagcaatacc tggttaggtg gcgcggaggg cgttcccagc
     1261 gcgttacgca ataaaccaaa aacaatgatg atgcgggtga aactggtcat catcagtaaa
     1321 attgccggaa taaacgtcaa cgaggtgatg aacaccagcg tctgcaccgg gagcgaccag
     1381 ctttgtccac cgccaggcag cggctggctg gtgatacccg gcagttgcgc gaaggcgagg
     1441 ggcgtaacca gccagagaag gacaggtgcg acagacaaca aacgacgcat caggatctcc
     1501 cactacgctt aagcaaattt ttcatgaccg actgaaaatc ggtctgcggt atctcttctg
     1561 ttggtgctga agggggaagt ttatgcagca gattgatttg ccctgcggta acgccgagca
     1621 ccagccgtgc atcttccaca tcgaccacca caacccgttc acgcgcgccc agcgaagcac
     1681 tggcgctaat tttcagaccg ttaacgccag tgcgtttcgg ggcaaatccc aaccgtttta
     1741 cctgccaggc agcagcgagg atcagggcaa taatggcgat gagtgcgccg ctgacctgca
     1801 gcagtggcgc agcagaaacc ggcgtggaag attgcacagt agcgtggtta ttcatcacta
     1861 acggctcagg cgatgcattc gctcagacgg agtaatgata tcggtgatcc gcacgccata
     1921 tttatcggca acgaccacca cttcgccctg ggcgattaaa taaccgttga tcagaatatc
     1981 cagtggttcg cccgccagac cgtccagcgc cacgacggac ccttgcgtca gacgcaacag
     2041 ctctttgatg gtcatccgcg tacggcccag ctcgacggtc agcttgaccg gaatatccat
     2101 tatcaggtcg atatcctgca acgttccgct gacatcacca ccgccaaatt gctggaacac
     2161 cgcgtcggca gcgcttttgc tgctggttga tttttgttcg ctcaacgctt cagcccacag
     2221 atcgtccatt gcgccgttgt tgtcatcggc cggattattc atgtcactca tttgggctgt
     2281 tcctcgttca gagaatttaa aatcgggtta atcaaatgtt ctatccgtaa cgcatactga
     2341 ccgttgagag tgccgtactg actggtcagc accgggacgc cgtcaacatg ggcgatgatg
     2401 cgatcaggtt tttctatcgg caggacgtcg ccgggtttca gttttaaaat ctgcgacagg
     2461 cgtagcgaga tatcggcaaa gttggcgacc agctccagct gtgaatgctg cacctggcgc
     2521 accaggttat cgcgccagtt ctgatcttca ttacgcgagt tttccagcgg cgggttaacc
     2581 aacaattccc gtagcggctc gatcatgctg aatggcagac agatattaaa ttcgccggtc
     2641 aggttgccaa tctccacatg gaacggcgtg ttaaccacaa tgtcgttcgg cgaggtggtg
     2701 atattggtaa atttcacctg catttccgaa cgcacgtact caacttccag cggattaatc
     2761 gccttccagg cgtcgctata gccttcaagc gccagcttta acatgcggtt gatgacgcgc
     2821 tgttcggtat gggtaaactc gcgaccttcc actttggtcg ggaagcgtcc atcgccgcca
     2881 aacaggttat ccacggcgat aaacaccaga ctcggtgaaa acaccaccag ccctgtgccg
     2941 cgcagcggtt tcagatggat aaggttcagg ttggtcggca ccggcaggtt gcgggcaaat
     3001 tcatggtacg gctgaatgcg gatggccccg acggttatat ccgggctacg acgcagcagg
     3061 ttgaacagcc ccatacgaaa atggcgggca aagcgctcat taatgatttc cagcgcctgc
     3121 aaacgttcgc gcacaacccg tcgttgggta ttcggatcgt acggacgaat gtcactttcg
     3181 ccgctaacac tggctgtcgg ttcgtctttg acttcgctgt caccattcaa cagcgcatca
     3241 atttcagctt gagaaagaat actatcgccc atgtcgttat cgcagaataa aagcggtata
     3301 cagcacgtcg gtgacatcct gtttcggttg cccggcaaca agcggggtgg aaagcgtggt
     3361 tttaatctcg gcaatcaggt ttttcttgcc ttcttctgtc gccagtacgg cagcatcctg
     3421 acgcgaaaac agtaacagca aacggctacg gacttccggc aaatactcac tcagccgcga
     3481 gcgggtagct tcatctttca ggcgcagggt tatgccgata taaagcacgc gatccgcatc
     3541 gcccaaattg accgtgaagg tattcagcgc gtagaagacc ggggagggta cgacgcgttg
     3601 ctgcgctttg tcgtcagcgg caacctgatg cgaatgccag tagctgtaac ctgcgctggc
     3661 acaggccgcg agggtaatga ataccagaat cgggatccaa agcgatcgct tgcttttctt
     3721 gcttatcgcg taatcagtca tgtgttgcgg tcttcctgtg tcgctactgc ttatcggcta
     3781 attatcctgc gttttgagcg caacaaagcg gggaaaagac gcggattacg gtgctacctc
     3841 tgacgttagg cgaaaatatc aacgccgctg ttgcctgtta cacgcccttg taaagagacg
     3901 ggaaccggaa gcgtatcgtc gtcttccccc gccagaggtt catggtttac tgtgcgttgg
     3961 ctttgctgtt gttgggaagc ggcctgctgc tgaccactaa agctttcgcc actgatgttg
     4021 ctttgcccta actgaatgcc actttcggcc agctgcgtac gcagtaccgg aagcgctgct
     4081 tccagcgcgg cgcgtacatg ctgatgcggt gaaaccatct ggatttgcgc ctggttatca
     4141 tccactttga gggagatttg cacttcacct aaatcctgcg ggtgcagacg caactctgca
     4201 ctttgttgcc cctggcgggt gaacagcgaa atatgctggc ttaatgattg ttgccattcg
     4261 tgagaaccca gcggtgcact caaaacaggc gcggcgacgg tgggcagtgg ctgtgtctgg
     4321 tgtggagtaa ttagcgggct ggcggcagcg gtcaccggtg acggtgtgct gatgacttcc
     4381 gctttactct gggcttctgc taccagcggt gttaatggct gagctggcgt gccgggggca
     4441 tcatcaggct gtgctgttgt gagttgcgca gaagtcagtt ttgtgaagag cgttggtttc
     4501 tctgccggta acacggttga cggcacatca gtcactttgg gcgtattgtc aaaacccggc
     4561 aacatcgcaa aaagggcgct caggcttgcg gtgacgtctt cattcagatc atccgctttt
     4621 tcgtcttttg tcgtattttg gtcagccacc gcagccaacg tcatcgtctg agcggtggtt
     4681 aacggtgttg atgtggattg ttcgtcgttg atgacaggca gtgtttcatc cacggggatc
     4741 agtaaatccg cttgttgcgc gtcggcaaga atatcgctga tcagcggctc gccttttgtc
     4801 gtgggcttat ctgttgccac cagcaactgg ggggccgctt tgtcggtagt tgtctcgcct
     4861 gctaatgctt cgctcaacaa cgcgagaaaa tcttgtgcag catcgctggc tttgccgcca
     4921 ggcaatgtgg tggtgtcaac gtcggcggta attaagggcg ctaagcgaat cattcaggtt
     4981 tcctcatggc ggcgcgctgg gcgaactcat ccatcttttt ctgatcgagg cggttttctg
     5041 caagcagtgc cgccgtggat agccgttcct gcagtgtctg ccaggcctgc aaacgttgtt
     5101 tcttttctcg ccagctgttc agggcaatgt caactttctg cgtccactga ttaagttgct
     5161 ggcgatgctg agtaatggct ttttccagcg tctggataaa ctgctgatag ttgatccagc
     5221 ggttgctggt catcccggca ctcatatcgc tgttgaggtt attgcgatat tcattctgat
     5281 aatcgatcag cattttgagc tgttcttccg cctgctgaca cccgcgacgc atttcaccca
     5341 gcaggcgcgc ggcatcctct acctcttttt ctgccagatc tttcagggtc gccagcgcgc
     5401 catgttctgc catctgccgt tatctcctgg gttatgacac tgtcgggaaa atacgctcca
     5461 gcccctggag agacgcttcc cagtccgcgc gttcaaaaat gccttgttgc aaatagccct
     5521 ccagctgcgg ccacagggcg atggctttgt cgagcatcgg atcgctgcct ttggcatacg
     5581 cgccgacgct aaccagatcg cggttacgct gaaaactcga caacagctgt ttgaaggtgc
     5641 gcactcgcgc gtaatgttgc tcactgatca acgccgtcat tgcgcggctg atcgacgctt
     5701 caatatcgat agccggatag tgcccggctt ccgccagtcg gcgagacagc acaatgtgac
     5761 cgtcgaggat tgcccgcgca gagtcggcaa tggggtcctg ctggtcatcg ccttcagtga
     5821 gcacggtata aaacgcggta atcgatccgc cgccgctaat gccatttccg gcacgctcga
     5881 ccagtgccgg taatttggca aacaccgacg ggggataacc tttagtggcg ggtggttcgc
     5941 caatcgccag cgcaatttca cgctgggcca tcgcgtagcg ggtgagggag tccataatca
     6001 gcaacacatg ctgaccacga tcgcgaaaat cttcggcaat gcgcgtggca taggcggcac
     6061 cctgcattcg caggagagga gaaacatccg ccggagcggc aatcaccacg gagcgtgcac
     6121 gcccttcggc accgaggata ttctcaataa aatcttttac ttcgcgtccg cgttcaccaa
     6181 tcaaacccac gacaatgaca tcggcgcggg tgtaacgtgc catcatcccc agcagcacac
     6241 ttttaccaac gccggacccg gcaaacagcc ccatacgctg cccacgccca acggtaagca
     6301 gggcattgat tgggcgcacg ccggtgtcca gcacatgttc aatcggtgta cgttgcaacg
     6361 ggttaaatgg cggggtaatc agcgcaccgg tttccgtcgt atcgggggag gccatcgagc
     6421 ggtttaccgc tgccgtccag aacacgacct aataacgccg gaccgagcgg taactgtttg
     6481 ccgctttgca gcccttctgc cgaaatgttt ttggcataaa cacgcgcgcc gggcaggaca
     6541 ccttcgactt cctccagcgg cattaaaaac agccgttgac cgttaaagcc aacgacttca
     6601 ctttctactt cgtgcgtttc agtgccgttc tggcgctcaa tcacacaggt tgcgccgagc
     6661 ggcaattgta atccggtggc ttccagcacc agcccggtag cgcgggttaa tcgcccgtag
     6721 cgacgtaccg caggcaactg cgccattttg gcttcaaagt tatccagcgt ggttagccag
     6781 cgagtcaggc gcgtggtcat tacaccactc ctggtgctgc cagacggcag agttcttgcc
     6841 agcgagtggc gacactggcg tcgagatcgc cttcatcggc ggagacttta cagccgccag
     6901 gatggagggt gggatcgccc cgcaagcgcc agccatgcaa acttaaggta gcgccgagca
     6961 tatcatccac acgttgcaga tcatccgggt gcacgcgcag ctgtggttta ccgctgaata
     7021 acggttcttg ctgcaacaac tgttggatct gtttgatgag tgccgagtta tccactgttg
     7081 gcgtctgacc gatgacctga cgtgctgcct ccagcgccat ctgcatcagg cgcgaagc
//
LOCUS       DAJTOR010000191         7105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTOR010000191 DAJTOR010000000
VERSION     DAJTOR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.191"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(826..1356)
                     /locus_tag="OO249_003631"
                     /pseudo
     CDS             complement(826..1356)
                     /locus_tag="OO249_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(1699..2349)
                     /gene="zinT"
                     /locus_tag="OO249_003632"
     CDS             complement(1699..2349)
                     /gene="zinT"
                     /locus_tag="OO249_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5637783.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2606..3241)
                     /gene="msrQ"
                     /locus_tag="OO249_003633"
     CDS             complement(2606..3241)
                     /gene="msrQ"
                     /locus_tag="OO249_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5637784.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(3242..4246)
                     /gene="msrP"
                     /locus_tag="OO249_003634"
     CDS             complement(3242..4246)
                     /gene="msrP"
                     /locus_tag="OO249_003634"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR5637785.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4355..4768)
                     /gene="hiuH"
                     /locus_tag="OO249_003635"
     CDS             complement(4355..4768)
                     /gene="hiuH"
                     /locus_tag="OO249_003635"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5637786.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4901..5572
                     /gene="hprR"
                     /locus_tag="OO249_003636"
     CDS             4901..5572
                     /gene="hprR"
                     /locus_tag="OO249_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5637787.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5572..6930
                     /gene="hprS"
                     /locus_tag="OO249_003637"
     CDS             5572..6930
                     /gene="hprS"
                     /locus_tag="OO249_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5637788.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASLGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYLNKHNVFRITLPQ
                     RN"
BASE COUNT         2005 a         1463 c         1604 g         2033 t
ORIGIN      
        1 gctgagaaaa aagcgtgctt tcccaatttg gggcaaatca cgtctgaatg acacctgaaa
       61 acaggtggtg gactcaagaa ggcttggaat attcttcttt aatgttatgt aattcattga
      121 tttttcgtgt acgattttaa gacatttttt ccaagaaaaa tttttaactc tttgattttt
      181 cgactctgta tcatcggtct cgaaaaccgg agtaggggca actctaccgg gggggcaaat
      241 ccccctctct ccgccacttt atcaatgact tatctcccga cttcccgcct tacttttcct
      301 aaacagaaca atcgtagaat attcttgaag ggttagatcg tcactgtttt ctgttcgata
      361 ctgtgacatt cagcacttga ttcgctatgg atctgacagg aagctttcga gcgaaaatgt
      421 gcagttattc agtcgttttc ttatcgctca ccattattct tttagacatt catcctatga
      481 aactgccgca aagttggtgg tgggaactga agttgcgtag agaaggggtc aataattgag
      541 tcaaaaaagg aacgcggccg tcagattaaa gccgcgggac aaagtccata atttccaaga
      601 gttgttgaat ttcccagctt ttttgaaagc ctttgtatca tactaacaag taaaaaaggt
      661 ttaaatacca aaattaacct gtatttttga taagtcttac cattgtacga tttacacagt
      721 ttctgctcct ccttgttgat agtggaaagg gtgcattgtg cacttgcgta aacatttatg
      781 acgcgttgat gtccatggtt cacatttcct tgtcagggga tcgacttatt tgcggcgagg
      841 catcattcgt aacagagtat tatctttctg aatgtagtga tgaaagagtg ctgcgccagc
      901 gtgagctgcg attaaaaagt aacctatatt tgcccaggtt tcgtgaatat tttatatcag
      961 tgctttaatt tcgctgtttg gggtaacaaa gggagacaca ttgaaaccaa ggaaactcca
     1021 cgattttcca ctgtaagcca tcaaagcaat ccccagcaga ggaagtgcaa ggaaggttat
     1081 ataaagcatg atgtgcatta gcgaagccgc tttcatttgc caggcaggtg gcggtggcac
     1141 aatagaagga tcactataac ggtgttttat aatcaggcgt gaaaacatta acacccaaac
     1201 gaatataccc gcattgtaat gtgtttctcg catcagcaga taagtactac tacctttagg
     1261 aaaccagcca cggagttcca tcgcggcata ggttattgca acgaaaagca gcgttatcca
     1321 atgtaaataa atttgagttt ttgagaatcg gttcattttt gtagctcctg taatttttac
     1381 agtcaagcta tcccacgaac cttaaaaatt ccttatacag atgtggtttt aagaatttgt
     1441 tatttaaaaa attgatagtg cacattgtaa ccagccaata taagcaatgg cgtgagcaca
     1501 ggtatctttc tctgttggcc gtattgtgta tgggatccgt tattggttaa ctcaatgcgg
     1561 gttagcttat taatatacag acacgtcata gtgatgagcg tgtctgtagc tagcttgcgt
     1621 tcagtggcgt ttgtggatgg accagtggca gttatgatga cttactaaag cggcatcgag
     1681 gcattatcat gagaatactc aatgagacat catttcctcg accacttctt cgctactcaa
     1741 ctgatatgga taatacgttg gccagttttc catttcattt aataaagatt gctgtgaatc
     1801 attacccata aaaatgtgaa aatgagatga ttttcgtggt gcaattatat gatcgctaaa
     1861 ttgtatatat ttaggggctt tgctttcagg atctttacat tcgaataagt aacgaacgcc
     1921 tttcttgcct gatttatagg tgagtatttt gtatccatcg taatcatatt tacaggatgt
     1981 tgtttcatta tttctatgga attcaacaat gccgtcctca atgccaatca tctcgatatc
     2041 tgttgcataa cctttgtgat aataatcttt aatttcagca aatgttttag ttttatctgc
     2101 atccgctttc ttctgaaaga cggggtcaag tttgccactt tgcagtaaag gataaacgga
     2161 ttgccagacc ccatcccagt cactgagcgt tcggttttgt acattggcat catcaaaaac
     2221 accattagca gctttttgtt cgacctctgt taagggtttg ccgtgtgagt gatgaccatg
     2281 cgaaaaggca ggagcgctaa caataaagac acctaacgca acagccagtt tgtgaagacg
     2341 aatcgccaaa acagtttcct ccagagtcat tgtatatgat gtgtaatgtt atattgtaac
     2401 atatggcaaa atgcaacaac tcttaggaga tgagagcata gttttctcag gtgtacttat
     2461 ctatcttatt gaaataaaat gaattatatt gataatttga ggttgatgac aaacgaaaca
     2521 taatctgatg tgatctgcgt aggccggata gggcgctcgc gccgcatccg gcagggataa
     2581 cgcgtacttt gtcggcaata tgaagttata cagataattt gttgtgaacc tgcttgcgta
     2641 accggttaaa aagggaacgc aacttcttat accgtaaggc taaaagcagt acagccagcc
     2701 cagcgtagat gagcggctgc ggtgaaataa tcttcactga ccacagataa tgtattgggg
     2761 ccaggatcgc gacaagatag acgaaattat gcaactgttg ccagtgcttg ccgagttttc
     2821 gctgcatcgc ctgggttgaa gtaaacgcta aagcaagcaa aataacccag ctgataattc
     2881 ctaacgttaa ataaggccgg gtaattaact ccttgcctaa taacgccaga ttattcacgc
     2941 ccagctccag caatgcgtaa ctggttaaat gcagtgtcgc ccaggcaaag caccataagc
     3001 ctaacaggcg gcgagtgcgt atcaataacg gctgttttgc gtagcgtgcc agaggtgtga
     3061 ttaacaaggt cgccagcaaa aatttcagtg cagtgcgacc ggtaaaatgc tgaatatctt
     3121 tcaccggatc ggcacccagt ccaccgtgat tgatcgccca gaccagccag agaaacggca
     3181 acaatccggc aagatgcagg ctaactttca gccatgtcac ctgttttgct gtcagacgca
     3241 ttcagaaatt ctcccgcaaa tccaggccac gatacagcga tgccacctgg tcggcgtaac
     3301 cattaaacag tagcgttggc tggcgctgaa catcgaggat gccgcctgaa ccaataaatc
     3361 gttcggtagc ctgtgaccag cgcgggtgat caacatgcgg attaacgttg gcgtaaaaac
     3421 cgtattcgtc aggcgctgcc agattccagg tggttggcgg acgctcgcgg gtcagcttaa
     3481 tactgactat cgatttaatc cctttaaagc catatttcca cggcacaatc agtcgtaccg
     3541 gcgcgccatt ttgtggcggt aacgccttgc cataaacacc cacggtcatc agtgtgagcg
     3601 gatgcattgc ttcgtcgaga cgcaatcctt cgacataagg atatttcagc ccgccgccga
     3661 taaagcggtc ctgctggcct ggcatttgtt ccggtgcata aattgtttcg aaagcgacat
     3721 acttcgcatt gctggtgggt tcggcaagcg ccagcaattt gtgcagcgga aaaccaatcc
     3781 acggcaccac catcgaccat gcctccacgc agcgcatacg ataaatacgc tcttccagcg
     3841 ggaagcgacg ggttaaatca tcgtgatcga gggtcaatgg ttttgccact tcgccgctga
     3901 ttttcagtgt ccatggatcg gttttcaggc taccagcatt agcggcggga tcggctttat
     3961 ccagcccgaa ttcatagaag ttgttataac cggagacttt atctactggc gtcagtggca
     4021 ggttattttg ccaggcggca ggcttgctga actccagcgg ttttccggcg ggggcgggcg
     4081 ggcgatcgtt ccctttaaac cagctaagca gatcggcatg cgcagcgtga ggcaaagaaa
     4141 gtgcagctgc actgatgccc agtgctttta acacctgccg acgcttcata aagaataccg
     4201 actcggccgt aacatctgat tcttttaaaa attgattctt tttcataagc catcacactt
     4261 ttttgtcgtt agccagcaag agccgtttac tgttaatctg tcagggagtg tcctccctaa
     4321 cagtagcaga gatctgcttt tacatattta tgcattaact gccacgatag gttgaatacc
     4381 catattggct aagtaataaa ggcacatgat aatgctcgtt cactttatta atatgaaatt
     4441 caacggggat ctcagggaag aaactttcaa gattttgttt cttgaaatag tccccggttt
     4501 taaatacgac acggtaatcg cccgtagttg cagtttgctc gggccacagt gctttaattc
     4561 gtccatcctt atctgttttg gcggtattaa gttgtaacca gccgttatcc gccttctttt
     4621 caagagtgac tgtcacgtca gcagcgggct ttccggtttg ctggttcaaa atgtgcacgc
     4681 taagaatgtt ttgttgtgcg gcataaacca aagaaggtaa tgaaaatgct gccgttgcta
     4741 ccgagagtac taaataacgc tttaacatgt ttatatcctt gtcatgtgaa tgagttttga
     4801 ggatagcgaa aaagcggcca gggtaacctt acagcagcat tacaattttg taatgaacca
     4861 ggctgtttct ataacatatg atttatggca tattattttc atgaagattt tacttattga
     4921 agataatcaa aggacccagg agtgggtaac gcaggggctt tccgaagcgg gttatgtcat
     4981 tgatgccgtt tctgatggca gagatgggct ttatcttgcg ctgaaggatg attatgcatt
     5041 gataattctg gatattatgc ttccgggtat ggatggctgg cagatcttac aaacgttaag
     5101 aacagcaaag caaacccctg ttatttgcct tactgcaagg gattctgtcg atgacagagt
     5161 cagagggctg gacagtggag caaatgatta tctggtaaaa cctttttcat tttctgagtt
     5221 gctggcaagg gttcgggcac aattaaggca acatcacgct ttgaattcaa cattagaaat
     5281 cagtggctta agaatggact ctgtaagtca aagtgtgagc agggacaata tcagtattac
     5341 actgacgcgc aaggagtttc agttactttg gctactggcc tccagagctg gcgaaattat
     5401 acccagaacg gttattgcga gtgaaatttg gggaatcaac tttgatagtg ataccaatac
     5461 ggtggatgtc gccattcgca ggctccgcgc aaaagttgat gatccttttc ctgaaaagct
     5521 aatcgccaca atccggggga tgggctattc attcgtagcg gtaaaaaaat aatgaaaaga
     5581 ttgtctataa ccgtccgttt aaccttgctt tttatattgc tgctgtctgt tgctggcgct
     5641 ggaattgtct ggactctcta taatggcctg gcaagtgagt tgaaatggcg cgatgataca
     5701 acactcatta accggacagc acagatcaag cagttgttaa ttgatggggt aaatccagat
     5761 acgttacctg tatactttaa ccggatgatg gatgttagtc aggatatctt gatcattcat
     5821 ggtgatggca tcaataaaat tgttaaccgg acaaatgtca gtgatgacat gttaaataac
     5881 atacctgcta gtgagacaat cagcgcagct ggcatttaca gaagcattat taatgatact
     5941 gagattgatg ctttacgaat taatattgat gaagtttcgc catcattaac ggttactgtg
     6001 gctaaattgg cttcagctag acataacatg cttgaacagt ataaaattaa tagcattata
     6061 atttgcattg tcgccattgt actttgctca gtattaagtc cgctgttaat cagaacggga
     6121 ttacgagaga tcaaaaagtt gagtggtgta acggaagcgc tgaattataa cgatagccga
     6181 gagcctgttg aggttagcgc attaccgaga gaactaaaac ctcttgggca ggcgttgaat
     6241 aaaatgcatc aagccttagt caaagatttt gaacgcctaa gtcaatttgc tgacgatctc
     6301 gctcatgaac ttagaacgcc cattaatgca ttactgggtc agaatcaggt tacgctcagt
     6361 caaaccagaa gtatcgctga atatcaaaaa acaattgccg gtaacattga agagttggaa
     6421 aatatttcgc ggttaacaga gaacatactg tttcttgccc gggcagataa aaacaatgtt
     6481 ttggtgaaac tggactcgct ttctctcaat aaggaagtcg aaaatttgtt ggattatctt
     6541 gaatatcttt cagacgagaa agagatttgc tttaaggtcg agtgcaatca gcaaatcttt
     6601 gcggataaaa ttttactgca acgaatgtta tcgaatctta ttgttaatgc cattagatat
     6661 tcaccagaaa aatcgcgtat tcatataacc agttttcttg ataccaacgg ctatcttaat
     6721 attgatatcg ccagtcttgg aacgaaaata catgagcctg aaaaactctt ccgtagattt
     6781 tggcggggag ataattcgcg tcattccgta ggtcagggac ttggtctttc tttagtcaaa
     6841 gcgattgccg aattgcatgg gggaagtgct tcatatcact atctcaataa gcataatgtg
     6901 ttccgaatta cgttaccgca aagaaattaa catagggctt cagtacgcca taaattaacc
     6961 gtgaaaaaac gcagccacgt cgtgagtact aacgcggctg cgattaatta tgcgcttaca
     7021 ttcaaacgta acagggatta acccgcgtaa gctgccagca tttcctgcgc cgccagttta
     7081 cccaacgcat tcgctgcgaa aggac
//
LOCUS       DAJTOR010000192         7019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTOR010000192 DAJTOR010000000
VERSION     DAJTOR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.192"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..337
                     /locus_tag="OO249_003638"
                     /pseudo
     CDS             1..337
                     /locus_tag="OO249_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            334..2334
                     /locus_tag="OO249_003639"
     CDS             334..2334
                     /locus_tag="OO249_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR5637789.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSGRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTAV
                     GFYRLAHVLVQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            2459..2920
                     /locus_tag="OO249_003640"
     CDS             2459..2920
                     /locus_tag="OO249_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR5637790.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASISATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(2961..3431)
                     /locus_tag="OO249_003641"
     CDS             complement(2961..3431)
                     /locus_tag="OO249_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5637791.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3478..4197)
                     /gene="yehT"
                     /locus_tag="OO249_003642"
     CDS             complement(3478..4197)
                     /gene="yehT"
                     /locus_tag="OO249_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5637792.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(4194..5879)
                     /gene="btsS"
                     /locus_tag="OO249_003643"
     CDS             complement(4194..5879)
                     /gene="btsS"
                     /locus_tag="OO249_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5637793.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLQLPWRDEA"
     gene            6101..6166
                     /locus_tag="OO249_003644"
                     /pseudo
     CDS             6101..6166
                     /locus_tag="OO249_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            6394..6642
                     /locus_tag="OO249_003645"
     CDS             6394..6642
                     /locus_tag="OO249_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5637794.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(6758..>7019)
                     /locus_tag="OO249_003646"
     CDS             complement(6758..>7019)
                     /locus_tag="OO249_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5637795.1"
                     /translation="QHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQDAVD
                     LEIATEEENSLLEAWKKYRVLLNCVDTSTAPDIEWPEEPDTM"
BASE COUNT         1786 a         1794 c         1721 g         1718 t
ORIGIN      
        1 gagttattaa tgaaagtaca gttgggcggc gggaccaata tcgccagtgc cgtggagtat
       61 ggtcggcaac ttattgaaca accagcgaaa agcgtcatta tcctcgtgag cgatttttac
      121 gaagggggtt catcatcatt actgacgcat caggtgaaaa agtgtgtcca gagcggcatc
      181 aaagtgcaaa gtgctgggac tggcagcgct cgatagcaca gcaacacctt gctatgaccg
      241 cgatacggcc caggcgctgg ttaatgtcgg cgcacaaata gccgccatga caccgggcga
      301 gctggcatca tggcttgcgg agaatcttca gtcatgaatt cactacgtcc ggaattatta
      361 gaactgacac cgcaggccct gacggcgtta agcaatgccg gttttgttaa gcgcagtctt
      421 aaggaactgg aaaatggcaa cgtcccggag atcagccatg agaacggcgc tttaatcgcc
      481 accttcagtg acggtgtccg tacccagctg gcgaacggcc aggcactgaa agaggctcag
      541 tgcagctgcg gggccagcgg tatgtgccgt catcgcgtga tgctggtgtt aagttatcaa
      601 cgactttgtg ctaccgccca gcctacggga aaagaagagg aatgggatcc ggcaatctgg
      661 ctggaagaac tggctaccct tcccgatgcc acccgcaaac gcgcacaggc gctggtcgct
      721 aaaggcatca ccattgagtt gttctgtgcg ccgggtgaaa ttccctctgc ccgcttaccg
      781 atgagcgatg tgcgttttta ttcccgcagc agtattcgtt tcgcccgttg tgattgtatt
      841 gaaggcacac tttgcgaaca tgtcgtactg gcagtacagg cctttgtcca ggcaaaagcg
      901 cagcaagcgg aattgactca tttaatctgg cagatgcgca gcgaacacgt cacatcatct
      961 gacgatccgt ttgccagcga agaaggcaaa acgtgtcgtc aatatgttca acaactaagc
     1021 caggcattat ggctgagcgg catcagccag ccgcttattc actacgaggc cgctttcagc
     1081 cgcgcgcagc aagcagcgga acgctgcagc tggcgatggg tgagtgaatc gctacggcaa
     1141 ctgcgcgcca gcgttgatgc cttccacgcc cgcgccagcc actatcatgc cggagaatgc
     1201 ttacgtcagc ttgcggcatt aaacagtcga ttaaactgcg cacaagagat ggcccggcgc
     1261 gacagtgttg gtgaagttcc tcctgtgccg tggcgcacgg tcgttggctc tggcattgcc
     1321 ggagaagcaa agcttgatca tctgcggctg gtgtctttag gtatgcgttg ctggcaggat
     1381 attgagcatt atggtttacg catctggttt accgatcccg acaccggcag tattttgcat
     1441 ctttcgcgta gttggccgcg aagtgaacag gaaaactcac cggcagctac gcgtcggctg
     1501 tttagttttc aggctggcgc actggcgggt gggcaaattg tttcacaagc agcaaaacgc
     1561 agtgccgatg gcgagctgct gttagctacc cgcaaccgct taagcagcgt tgtgccgctg
     1621 tcgccagacg cctggcaaat gttgagcgcg ccgttacgcc agccgggcat tgtggctttg
     1681 cgggaatatt tacgccagcg tccccccgcc tgcatacggc ctcttaatca ggtggataac
     1741 ttatttattc tgccggtcgc tgagtgtatt tcactcggtt gggacagcgg ccgccagacg
     1801 ctggatgcgc aggtaatcag cggcgaaggt gaagataatc tgctgacgtt atcccttccg
     1861 gcatctgcct gttctccttt tgccgttgaa cgcatggcgg cgcttttgca acaaacagac
     1921 gaccccgtga gtctggtttc tggctttgtc agctttgtag aggggcaatt gacactggaa
     1981 ccacgggtga tgatgacaaa aacccgtgcc tgggcgctgg acgcggaaac tgcgcctgtg
     2041 gcaccgctac cttctgccag cgttttgcct gtgccgtcta ccgctcatca gttgctgatg
     2101 cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca ggaacagagc
     2161 gctattagtc aggcagagtt gctggcgaat gacctcaccg cggtgggttt ttatcggctg
     2221 gcacatgtgt tggtacaatt tcgtaataca gaaagcgagg cacgggtaga agcaatgaat
     2281 aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg atgaaatagt
     2341 gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta ttaggcatat
     2401 cctatatagc ccctggtgag agtcaccagt ttcttgatta aataaaatgg agttttacat
     2461 gaaggctttc aataagctgt tttccctcgt tgttgcatct gttctggttt tctctcttgc
     2521 tggctgcggt gacaaagaag aatcgaagaa attcagcgcc aatctgaacg gcactgaaat
     2581 tgccattacc tatgtctaca aaggtgacaa ggtgcttaag caatcttctg aaaccaaaat
     2641 tcaatttgcc tccattagtg caaccaccaa agaagatgct gccaagacac ttgagccgtt
     2701 aagcgccaaa tacaaaaaca tcgcgggtgt tgaagaaaaa ttaacctata ccgataccta
     2761 cgcgcaggaa aacgtgacta tcgatatgga aaaagtggat tttaaagccc tgcagggtat
     2821 ttcaggaatc aacgtttctg ctgaagatgc caaaaaaggt atcactatgg cgcaaatgga
     2881 actggtgatg aaagccgctg gttttaaaga agtgaaataa tctgtcggcg gccatgtttc
     2941 gtatggccgc catacccgtc ttagcttttc ttcacatgct ggcgcgctgc cagtccacgc
     3001 agaaaataac gtaaaaattg atcgccgcat tcgcggaagt ttttatgatc cggtgcgcgc
     3061 atcatcgctg taatttccgg catcgaaacg cggaactgct gttcggtgag gatagccaga
     3121 atgtcatcgg ttttcagcga aaacgcgatg cgtaattttt tcagcacgat gttgttatta
     3181 atgcgacgtt ccggctccag tgccggagca gactcatcct tgccgcgttt ttcataaatc
     3241 aggccattga ggaatgacga caaaacaatg tccggacaac gctgaaaacc ctcttcgtct
     3301 tctttacgta gccagacggc gatctgttcc gcggtggctt cgacattacc cagcgccaga
     3361 atacgcacca ggtcattatt attggctttc aaaatgtagc gcacgctgcg cagaatatcg
     3421 ttacttagca tgaggccttc aggtgttgat gaggcaaaaa gccattttag cagtctttta
     3481 caggccaatc gcctctttta agcttttcag ataacggcgg ctgaccggca cggttaagcc
     3541 attacgcaaa atcaactcgg cctggccgtt atcttccaga cgaatctcct gtaaatgcgc
     3601 gaggttaacc agatactgac gatggcagcg cagtagtggt gtacgacttt ccagggtacg
     3661 taatgtcaat tcggtaaagc cctctttccc ttcgtggctg gtaacgtaga caccgctcat
     3721 ccgactgctg acaaatgcca catctttcat ttgcagcaaa taaatccgac tatgccccgt
     3781 acaagggata aatttcagcg cctgttgatt ttccggtaac agcgaaacat cctgcttgct
     3841 gcgctcctga cgcaatcgcg ccagcgtttt ctccagtcgc gcttcatcaa ttggcttcag
     3901 cagataatca aaggcatgtt cttcaaaggc tttaatagcg tattcgtcaa acgcagtgag
     3961 aaaaacaata tacgggcgat gttccgggtc aagcatcccc accatttcca gaccactgat
     4021 gcgcggcatc tggatatcga gaaacagcac atccgggcgc agtttatgca ccgcgccgat
     4081 cccttccacg gcgtttgaac actctccaac gatttcaata tcgctctgct cctgcaaaaa
     4141 tacacgcagg ttctcccgtg ctaacggttc atcatcgaca attaagactt taatcatgcc
     4201 tcgtccctcc atggtagttg taacgttatt cgggtgtaac tatcaggctc acaggcgacg
     4261 cttattccat agtcatcgcc aaaccgttca cgtaaacgct tatccaccag attcatcccc
     4321 agcccactgg cattggttac cggttgatac aaaccggcat tgtcttcgat ctccagcatc
     4381 aaatgttgcc cctcacgtcg ggcgctgatt gccactcgcc ctgtatccag cagttgtgat
     4441 gtcccatgtt taatggcgtt ttccactatc ggttgcaggg taaacgcggg caattgctgc
     4501 tgggataatt cttgcggaat agcaatgttg acctgcaacc gcgactggaa gcgcgccttt
     4561 tcaatttgca gataagcgtt cacatgttca atttcgtcgg cgagagtaac aaactccgaa
     4621 ggccgcttta agtttttgcg gaaaaaagtg gaaagatact gcaccagctg gctggcctgt
     4681 tcgctgtcgc ggcggatcac cgctttaatg gtgttaagcg cattaaacaa aaaatgagga
     4741 ttcacctggg cgtgaagcag tttgatttct gactgggtga gcatcgcttt ttgccgctca
     4801 tattgcccgg caagaatctg cgccgaaagc agttgcgcaa tcccctcgcc cagcgtgcgg
     4861 tttattgaac tgaataaacg gtttttggct tcatacaatt tgatggtgcc catcacccgc
     4921 tgattttcac cacgcaacgg aattaccagc gtcgacccca gtttgcattg cggatgcaaa
     4981 gagcaacgat aaggtacttc gttgccatca gcgtagacca cttcaccggt ttcaatcgct
     5041 tttaaggtgt aagtcgaaga aatcggtttg ccgggtaaat ggtggtcgtc accaattcca
     5101 gtaaaggcca gcaatttctc tcgatcggta atcgcgactg caccaatatc cagctcctga
     5161 tacagcacct gtgccacttt catgctgttc acttcgttaa acccctggcg caaaatgcct
     5221 tccgtcgagg cagccacttt cagcgcagtg gcagaaaaag ccgaagtgta tttttcaaac
     5281 atcgcgcgtt tatcgagcaa tatacgcata aacagcgccg cgccgacggt attggtgacc
     5341 atcattggtg cagcaatatt actcaccaga cgcaccgcat cttcataagg tcgggcgatc
     5401 gccaggatga tcaacatttg caccatttca gcgacgaacg tgacggcacc ggcggtaatg
     5461 gggttaaaga ctttatcagt acgcccgcga cgaatcagga tgctgtgtac caggccgccg
     5521 agcaatcctt caacgatggt cgagatcatg caacttagcg cggtcatgcc ccccatcgaa
     5581 tatcgatgta acccgccggt caggccaacc agcccaccga cgaccggacc gccgagtaag
     5641 ccgcccatta ccgcgcctat cgcacgggta ttggcaatag aatcgtcaat gtgcaaccca
     5701 aaccaggtgc ccatgatgca gaagatggaa aagacgatgt agcagagaaa tttatgcggc
     5761 agacgaaccg tgacctgcat taacggtatg aataatggcg ttttactcat taaccacgca
     5821 atgactaaaa aaacgcacat ctgctgaagc agcagcaaca ccagattaaa atcgtacata
     5881 cccgcaaacc acacttccct ttaaaacgcg taacatacat tgcctgcgtt taactttctt
     5941 tgaactcttg cagaaaaatg agaattcgtg agtacgatca ctcaaaatcg cctggcaaaa
     6001 ataaaatcac cctatagatg cacaaaaaac gggcaaaact acctggttcg caaaactgcg
     6061 tctaaagtta aaccgggacc tcgcgagcaa gggtgagacg atggcgcttt acacaattgg
     6121 tgaagtggcg ttgctttgtg atattaaccc tgtcacgtta cgcgcgtgac gcaacatttt
     6181 aaaaaatcga cgaagatcgt ccaggagcgc cgtttttcaa gggttggata gatgattcgt
     6241 ttaccatgtc gacgaacttc gacgaatttc gccgataaaa agtgtcccat acatgcccca
     6301 ttgatgcacc ataaacaata tcagaaattg atactggtta tccatacaga taaaaataac
     6361 acctgtacat aactacagtt acaggagggt gttatgcgtg ttgagatctg tatcgctaaa
     6421 gaaaaaatca ctaaaatgcc aaacggtgct gtggatgcgt taaaggaaga attaacccga
     6481 cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc
     6541 ctttctgtta cacgcaccgc agataaggat tctgcaaaaa catttgttca ggagactctg
     6601 aaagatacct gggagtctgc tgacgagtgg tttgttcact aataaacacg taaaatcggt
     6661 aacggctgga aatcattcaa tactcgcact atcgaaagtt taccagccaa ccgcagcacg
     6721 tcctgcatac gtcgtgtcta cggtttttct ttttcgctta cattgtgtct ggttcttccg
     6781 gccactcaat atcaggtgca gttgatgtat caacacagtt cagcaacacc cgatacttct
     6841 tccaggcttc cagcaacgag ttttcttcct ccgttgcgat ctccagatct acagcatcct
     6901 gaagtggcgc aatatgctca ctgaattcct ggatgtagaa ctgtgtggtg acggtcttcc
     6961 agccattcgg ctcctgctgt atcgaagcat accaggctat ttcaatatcg ctatgctgc
//
LOCUS       DAJTOR010000193         7016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTOR010000193 DAJTOR010000000
VERSION     DAJTOR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.193"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..237
                     /locus_tag="OO249_003647"
     CDS             <1..237
                     /locus_tag="OO249_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637796.1"
                     /translation="LFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILNTLN
                     KMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(206..1942)
                     /gene="aceK"
                     /locus_tag="OO249_003648"
     CDS             complement(206..1942)
                     /gene="aceK"
                     /locus_tag="OO249_003648"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR5637797.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASELWYSV
                     SPGDVFLEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2223..3520)
                     /gene="aceA"
                     /locus_tag="OO249_003649"
                     /pseudo
     CDS             complement(2223..3520)
                     /gene="aceA"
                     /locus_tag="OO249_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(3550..5151)
                     /gene="aceB"
                     /locus_tag="OO249_003650"
     CDS             complement(3550..5151)
                     /gene="aceB"
                     /locus_tag="OO249_003650"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR5637798.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFGDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            complement(5420..6349)
                     /gene="metA"
                     /locus_tag="OO249_003651"
     CDS             complement(5420..6349)
                     /gene="metA"
                     /locus_tag="OO249_003651"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5637799.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6506..6953
                     /locus_tag="OO249_003652"
                     /pseudo
     CDS             6506..6953
                     /locus_tag="OO249_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         1683 a         1802 c         1823 g         1708 t
ORIGIN      
        1 ctgtttggat ttaccgctga agatattatg gatttttggc aacacaaagc gccacaaaaa
       61 tactctgcct ttgagttggc ttttgaattt ggtcaccggg ttattgctga attaatcctt
      121 aatacattaa ataagatggc tgaaagcttt ggctttacgg ataaccctcg atacattgcg
      181 gagaaaaatt atatggaagc tttactcaaa aaagcatctc cccataccgt acgctaaatc
      241 tttgcctgcg ccgatacgca taaacatctt ccacatgccc ttcacgtatg cggttttgta
      301 gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac agcggaccaa
      361 tacgcgggtc ggcgcatagc cagtggcgaa actcttccag gaaaacatcg cccggcgaga
      421 cgctgtacca cagttcgctg gcaagttcgt cttccggata gcgcggcggc gggatgtcgc
      481 ggaaattcac ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc acacgcccgt
      541 gacgggtgac accgaagttt ttaaacagca tgtcgccagg aaaaatgtta gcagcggcaa
      601 gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg caactgctga ccttccactt
      661 gttccagcca gatattgagc ggcaccatcc gccgctcgat atagagatgg cgaattacaa
      721 tctgctcacc aagatcggtg attttttccg ctgcttcctg gagcagtaat tccattaatg
      781 ccggggaaat atgccgcttc tccagcacaa agttttcaaa ctcctgggtg tccgccattc
      841 ggcccacacg gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga gcggcagaca
      901 tctctttctg cggcgcgaac ttgtctttga tgactttgaa tacccgatca aagcccggca
      961 gcgtaaacac caacatcacc attccacgaa tacccggcgc ttcaatgaac tgctcattac
     1021 agccctgtag ataaacgaga tattcgcggt agctttcggt tttggcgtgc ttctggcagc
     1081 cgatagccat atacaattca gcagtggttt tacctggcag aatttcccgt agccactcga
     1141 ccagcgctgc gggcagcggt gcataaacca taaaataaga acgcgcaaag ccaaaaacaa
     1201 tgctcgcttc ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg tcgtccgtct
     1261 ggtggatcgg cagtaagaag ggcaacgtgc cggaaggcgt gatcagtttg cccaccagcc
     1321 aggcggcttt attgcggtaa aacagttcgt tcgccacctg taaatgactt tccgcgaggt
     1381 tgtctgtccc cagcgtttcc gtcagatggc gaatgatgta atgaatgtca cggcttttat
     1441 tctgccagcg caggcgcagc ggcaggtcgc tgataacgcg catcagtaga gattcccagc
     1501 cgtgatcggg gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag cggcgctctg
     1561 gctgagagct aaagataaaa agccgctcgg gagtaagcga gcggtggtca aataaccgac
     1621 agtacacgga gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc ggcaacagcc
     1681 gcgtgtaatg ctctttaacg cgcagtaaaa atgccgcgtc cgtactttgg ccgttagtaa
     1741 tgcagcgcag ttgctccacg accagaccaa cgtgatgatc gtaaagatgg atacggtttt
     1801 tcatcgccag ctggacagca tgccagtcgg cctgttcgaa acgctgctgc gccccggaag
     1861 tcacttcgag gaatcgacca tactgagcgt cgaagccttg caaaatggtt tgagcaatca
     1921 gtaattccag gccacgcggc atttagcgcc ctcatcagga gcagagaatt gccggatgcg
     1981 gcgtaaacgc cttatccagc ctacgttcgg caacggttgt aggcatgata agacgcgcaa
     2041 gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc gtaaatgcct tatccggcct
     2101 acgttcggca acggctgtag gcatgataag acgcgcaagc gtcgcatcag gcatcgtgca
     2161 ccgattgccg gatgcggcgt gaacgcctta tccggcctac agtcagcaac ggttgttgtt
     2221 gcttagaact gcgattcttc agtggagccg gtcagcgcgg tgactgaaga cgtgccgccc
     2281 tgaataatag tcgtcacttt atcgaagtaa cctgtaccca cttcctgctg gtgagatacg
     2341 aaggtatagc catctttcgc ggcggcaaat tccggctgct gcactttctc aacgtagtgc
     2401 ttcataccct cgccctggtc ataggcgttt gccaggtcaa acatgttgaa ccacatgctg
     2461 tggatacctg ccagggtgat gaactggaac ttgtagccca tatccgacag ctgctgctgg
     2521 aagctggcaa tagttttgtc gtcgaggttt ttctgccagt tgaacgacgg cgagcagtta
     2581 taagccagca gtttgcccgg atatttcgcg tggatagctt gtgcaaagcg acgcgccagt
     2641 tccagatccg gcgtggaggt ttcacaccag accaggtcag catatggcgc atacgccagg
     2701 ccacggctga tcgcttgctc aatgcccgca tgagtacgga agaagccttc actggtacgc
     2761 tcgccggtaa taaattcgct gtcatacggg tcgcaatcgg aggtgatcag atccgcggca
     2821 tcggtacgag caaccagtag ggttggaacg cccgtcacgt cagctgccag acgcgccgcg
     2881 accagtttct gaatagcttc ttgagttggc actaaaactt tgccgcccat gtgaccgcat
     2941 ttcttcactg acgccagctg atcttcgaag tgaactgccg ctgcaccggc ttcaatcatc
     3001 gctttcatca gttcaaaggc attcaggaca ccgccaaaac cggcttccgc atcggcaacg
     3061 atcggcagga agtaatcgac atagcgcgga tcgcccggct caatgcccgc ggaccattgg
     3121 atctgatccg cacggcggaa agtattgttg atccgctcca ccacagctgg caccgagttt
     3181 gccggataga gcgactgatc cggatacatg ctggccgcca ggttagcgtc cgccgctacc
     3241 tgccatcccg acagatagac tgcttcaata cccgctttcg cctgttgcag cgcctgaccg
     3301 ccagtcagtg caccgaggct gttgatgtag cttttttcga ctcgccatgc agcagacgcc
     3361 acattctcgc cgcgcccagt tgcgccaggg tgcattcagg attgactgaa tcgcgtaatt
     3421 tcaccacatc ttccgcgctg tatgggcgag taatgccttc ccaacgcggt tgagtccact
     3481 ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat gtgcagatgc tccatagtta
     3541 tgtggtggtt tacgctaaca ggcagtagcc tggcagggtc aggaaatcaa ttaactcatc
     3601 ggaagtggtg atctgttcca tcaagcgtgc ggcatcgcca aaacgcccct gggagaaacg
     3661 ttcttcgccc agttcgctgg caatgacttt catctcttcg cccagcatct ggcggaacaa
     3721 ggctttagtt accggtttgc cattgctcaa cattttttga tgatggatcc actgccagat
     3781 tgaggtacgg gaaatttcag ccgtcgccgc atcttccatc aggccataaa tcggcacaca
     3841 gccgttgcca gagatccacg cttcgatgta ctgcacggct acgcgaatat ttgcgcgcat
     3901 accttcttcg gtgcgttcac cgtcacaagg tgccagtagc tgatcggcag taatcggcgc
     3961 gtcttgttcg cgcatcactt caagctgatt tttacgggag ccgagaatgt cgttgaatac
     4021 cgccattgcc gtatccgcaa ggcctgggtg agcgatccat gtgccatcgt gaccgttatt
     4081 ggcttccagc gatttatccg cttttacttt gttgagcacc tggttattgt gctcttcatc
     4141 tttgctcgga ataaacgccg ccatgccgcc catcgcaaaa gcaccgcgtt tatggcaggt
     4201 tttaatcaac aggcgtgagt aagcattcag gaatggttta tccatcgtca ctgcctgtct
     4261 gtctggcagg acgcgatcgg gatagttttt caacgttttg atatagctga agatgtaatc
     4321 ccaacgaccg cagttcagac caacaatatg gtcacgcagc gcgtgaagga tttcatccat
     4381 ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc ttgatggtgc cgcgcggcag
     4441 attaaagcga tcttctgcat agctgaagac ttcgctccac caggccgctt cctgccagga
     4501 ctgggttttc ggcagataga aatagggacc actgcccttt gccaacagag cctgatagtt
     4561 gtggaagaaa tagagcgcaa aatcaaacag gctgccgggg attgcctcac cacgccaggt
     4621 gacatgtttt tccggcaagt gcagaccgcg tacccgacaa atcaaaaccg ctggattggg
     4681 cttgagctgg taaattttgc ctgcttcatt ggtatagctg atggtaccgt taactgcatc
     4741 acgcaggtta atttgcccgt cgatcacttt gttccagtcc ggggccagtg aatcttcgaa
     4801 atcggccata aagactttca cattggcgtt gagcgcgttg atcaccatct tgcgctctac
     4861 cgggccagtt atctctacgc ggcggtcttc taagtccgca ggaatcccgc gaattttcca
     4921 atcagcatcg caaatggaag ctgtttccga aataaaatca ggcaacgttc cgttatcaat
     4981 atcttgctgc tgctgaatgc gcgctgccag aagtttattt cgttgtggcg taaaatgcgt
     5041 caccagctca gtcagaaatt ctaccgcttc ggcagtaaga atttgcttct cctgctcgcc
     5101 atacggcctt gtgaaagcca gttcatcggt tgttgttgcc tgttcagtca tcgtgcagct
     5161 cctcgtcatg gatccgaaaa cttccccact gtgaacgaag gatcgttgtg cgcttttcgt
     5221 tcagcacaac taagactact catttaaaat gcaaaatcaa aaacaatttc cattttaatt
     5281 gaaaatgctt gataacttat tgataacaat gtgaataaaa tttattcacc tcctgaggtg
     5341 cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt aaggtgctga atcgcttaac
     5401 gatcgactat cacagaagat taatccagcg ttggattcat gtgccgtaga tcgtatggcg
     5461 tgatctgata gacgtaatag ttgagccagt tggtaaacag caaattaccg tgactacgcc
     5521 agctcgctcg cggtgtattt tgcggatcat tgtgcgggaa atagttatac ggtacttccg
     5581 ggtctagtcc ggcttccaca tcgcggaaat attcctgcgc cagcgtttgc gcatcatatt
     5641 cgggatggcc cgtcacaaag gcaatgcgct tatctttgct ggcaaacaga tatgcatccc
     5701 cttcttccgt ctctgccaga atttccagat cggtgtaatc acgaatcaac gctgccggaa
     5761 agtcagcata gcgcgaatgt ggtgccagga atgaatcatc aaagccacgc gtcagaagcg
     5821 catgaggatg gagaatatga tgctcgtaaa cgccagagag tttgtcggtg cgagtttgct
     5881 taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc ccagcagaca aacagcgtcg
     5941 aggtgacgtg atctttcgac cactccagca cctgtttgat ctgcggccag taagcgacat
     6001 cattaaactc caccaggccc agcggcgcac cggttacaat cagaccgtca aagttctgtt
     6061 cctgaatatc ttcaaagtta cagtagaagt tgttcagatg ctctgcgggc gtgttgcgcg
     6121 attcacggga atcgatgcgc aacagctgaa tatcgacctg caaaggtgag tttgaaagca
     6181 ggcgcagaaa ctgattttca gtttcaatct tcttcggcat caggttaagg atcagaacct
     6241 taagtggacg aatttcctga ccagacgcac gagaagttgt catcacaaag acgttttctt
     6301 cacgcaagaa attgacggcg ggtagctcgt ccggcacacg aatcggcata acctgattac
     6361 ctcactacat acacttatac gtttagacat ccagatagct gaagataacc agaaaatttg
     6421 ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa ggaaaagact acgttagaat
     6481 ataagaatga ctaaaaggag agaaaatggt tattagtatt cgccgctcac ggcatgagga
     6541 aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat gccacacacg attttttatc
     6601 agcagagtat cgggccgagc tggaggtgct agctagttcg ttccttcctg ccggaagcgc
     6661 cgttgtggat cgcggttaat gaacgggatc agccggttgg gtttatgttg ttaagtgggc
     6721 agcatatgga tgcgctgttt atcgatcctg atgtgtgcgg ctgcggcgtg ggtcggatgc
     6781 tggtggagca tgcgctctcg atggcaccgg aactgacaac caacgttaat gagcaaaatg
     6841 agcaggcggt tgggttctat aagaaggtgg gttttaaggt tacgggacgc tctgaggtgg
     6901 acgatttggg gaaaccgtat ccgttgctga atctggcgta cgtgggggaa taagccgccc
     6961 ctgccgtgcc tgcccaggct ggagaagacg cgccagcgtc gcacccggca tttttt
//
LOCUS       DAJTOR010000194         6942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTOR010000194 DAJTOR010000000
VERSION     DAJTOR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.194"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..528)
                     /locus_tag="OO249_003653"
                     /pseudo
     CDS             complement(1..528)
                     /locus_tag="OO249_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            671..793
                     /locus_tag="OO249_003654"
                     /pseudo
     CDS             671..793
                     /locus_tag="OO249_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1055..1621)
                     /gene="ipgB2"
                     /locus_tag="OO249_003655"
     CDS             complement(1055..1621)
                     /gene="ipgB2"
                     /locus_tag="OO249_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5637800.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1903..2217)
                     /locus_tag="OO249_003656"
     CDS             complement(1903..2217)
                     /locus_tag="OO249_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637801.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSAQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2295..2405)
                     /locus_tag="OO249_003657"
                     /pseudo
     CDS             complement(2295..2405)
                     /locus_tag="OO249_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2729..3406)
                     /gene="ospD1"
                     /locus_tag="OO249_003658"
     CDS             complement(2729..3406)
                     /gene="ospD1"
                     /locus_tag="OO249_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5637802.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGVPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(3777..3914)
                     /locus_tag="OO249_003659"
                     /pseudo
     CDS             complement(3777..3914)
                     /locus_tag="OO249_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3996..4294)
                     /locus_tag="OO249_003660"
                     /pseudo
     CDS             complement(3996..4294)
                     /locus_tag="OO249_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4883..5042)
                     /locus_tag="OO249_003661"
                     /pseudo
     CDS             complement(4883..5042)
                     /locus_tag="OO249_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5046..6200)
                     /locus_tag="OO249_003662"
                     /pseudo
     CDS             complement(5046..6200)
                     /locus_tag="OO249_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6220..6567)
                     /gene="tnpB"
                     /locus_tag="OO249_003663"
     CDS             complement(6220..6567)
                     /gene="tnpB"
                     /locus_tag="OO249_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5637803.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(6567..>6942)
                     /locus_tag="OO249_003664"
     CDS             complement(6567..>6942)
                     /locus_tag="OO249_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5637804.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLR
                     LKGKLTPALLQILIREIKGSSH"
BASE COUNT         1773 a         1631 c         1506 g         2032 t
ORIGIN      
        1 cgccacacca actgtgccgt cgccagcgat tgtaatcaca ttcgatataa ttaaacactg
       61 ttgcccgcat tatttcccgg ctgataaagt gttctccgtg gagacattcc actttcagcg
      121 aatgaaagaa gctttccacg caggcattat cgtagcagca gccctttgcg ctcatactgc
      181 cacgcagatt atgccacttc agcagcgcct gataatctgc tgaacagtac tgacttcctc
      241 ggtccgagtg aacgataacg ttccggggtc tcttacgccg ccacagcgcc atctgcaggg
      301 catcgcaggc cagttgcgcc gtcaggcgtg gtgacatgga ccagccaata acggcacgtg
      361 accacaggtc aatgaccaca ggtcaatgac cacagccaga tacagccagc cttcatctgt
      421 acgtaagtac gtgatgtctc ccgcccactt ctggttcgga ccactggcgt aaaaatcctg
      481 ctccagcaga ttttctgaca caggcaggcc atgtgcgcgg taactgaccg tattgctggg
      541 taagatcaat ttgctcactg gtatttacta cgtgcatcga atgagtgatc aagagccgta
      601 tgacgcgaga gtgtcacgta cggttccgtg agcagccgga gagtgaaact ctctccggct
      661 gactcgactc gcaggttccg acagcggtga cgaacatgca gcggtgttgt tctcgctgat
      721 cggcacatgc cgtctgaaca atgtggagcc agaaaaatgg ctgcgttacg ttattgaaca
      781 tatccaggac tgaccggcaa accgggtacg cgatctgttg ccttggaaag ttgatctgac
      841 ttctcagtaa atatcaatat gattctgacg agccgcttac ctttgagcag agaaagggga
      901 gagggatccc acatccatga gctaggaaaa tacaaaaact ggctatacaa caattcaata
      961 tattaggact tacttgcgag gagaaatata gcaccgctca ggcaattcct gggtatttac
     1021 ttttcttagt ttctttactc gggatgttct gtcatcagaa aggcgattct aaatttgtaa
     1081 tataatcact catattccca cgtaatttct ttagatcaat atattgcaca tttttctcaa
     1141 aaaactgatc ggaaataaga tttgcaacat tgcttgttgt atcattttta acgcttctat
     1201 tcatcccttc acaaagttcg tccattttct ttttgaaata actgcttcct gatacaagat
     1261 gatttatgga actttgtgca gcattgatgt ctagagaaat gtgataatct ttctcaacct
     1321 gtttaaatat ctgtttacgg agattatcgc cgatttttcg gctgtgttct gcacaaaact
     1381 tatcaataca ttggtttacc acccgggata cgtagacttt tttttcatca tttttccaat
     1441 ctgtaatatt ttcaccgatt tgttctgaaa cagagctcaa aatactagta tttattttgg
     1501 ttgatgatat ttttactatc cttttcccca ttgtacagcg gataatctca tcaacctttc
     1561 ctgaaaaata tcttttgtga gataaactga ttccaaaatt attaaaagat gttccaagca
     1621 ttataatcac tttgtcatgt aaaatagtaa cattgtcatt attttagctg attcaaacaa
     1681 ttctgtaaat gctttgagtt ctttactccc ttggctcaat gatggaccgt ctggaatccc
     1741 agagactaaa ctctctgaga aagagagtat gaaaaaactt ggggcagtcc ctaaatgaag
     1801 ggaccaacag cggtaaaata accggcatca ttcataatga tgccctgtta gttgtagtta
     1861 tgaagattta cgtcattaaa attgctgttc gtagtgtcag tcctatggtc tggcatcagc
     1921 tgaggatcgt agccgataca tcactggctt cacgacattc gtgttgaggc tattcatgaa
     1981 aactccacgc tgaaaacgcc tttttgtata aacggtcacg gtatgcccgg agccactgtt
     2041 gctgatgaat tcgataaaac cttggcgttt cttgaagcta tcgttaatgc ggataatgaa
     2101 acaacgattg gtgagattcg ctcttttgcc gacaccctgg gcgctgtccg gttcaatcgc
     2161 aacaaaatca accggcagct gagcaaaccc aaccttgcct ctctggcgct ggagcatgaa
     2221 gtgatttagc tgggacgtag ccactgacag cttctataaa agtatgatcc tacccacgta
     2281 atatgaacac aggcttaagt gaggttctgg ttttcaaatt gttcaggact gagacctcca
     2341 caccaactgt gccgtcgtca gcgattgtaa tcgcacttga tataattaaa cactgtatat
     2401 ttcatttgtg gagtctccag tctgaggggg ctttatgcat tccgtattgg gcactatgat
     2461 gccagaaatc tgcaaggctc cacaccttcc tgtatttccc ttcctctatc acagtgcact
     2521 ttacttaata gggtgggatg tgttcattac atttattgtg ttttttatga ttgtttttgc
     2581 gtccagtgtc gttcgtcatg ggaaactggt tctatgggta gtatcgctca gtctagttaa
     2641 ctgtttgttt ctttttagaa attaagcaga tcacacaatt cggggagagc tccttctaag
     2701 tgcaattctt tgcagctatt tagcacactt aaaacaactc ttgtattttg tcagaaataa
     2761 tattaaacat gtcttgttca ttattgttaa gtctgtctgc tatttctgta agttttatcc
     2821 cactatgatg gcatatattt atatatgcca tcatagcctc gatattccct ttccctgcgg
     2881 ccagaaataa tcctggtacc ccctcattat ctcttacatc caatagttta atcgtttctt
     2941 ttggtgataa atcaaaagtt ttaataaaat ctccataagc ttgaatcgca gcggaatgac
     3001 cttcttgaag agctatgaac aaagcagatt ccccagcatt atttcgtgct gcaagaactt
     3061 ctagtttttc ctctttgggt atttcggctg ccaacagcga ttcaccgtat ttacgcgctg
     3121 catcagcatt tccatgccaa atcaatgaaa acaaattatg gcggttatac tccttattat
     3181 tctttgtcgg aatggatatg ctctgtattt tggccaattt tctgaaagaa acaccactat
     3241 atcccccccc tgatttttct tcattgatgg cgtgggatat agcggtattt gtcacgttaa
     3301 tgatattacg tttcattcct ttattttcat tagcagtatt gctgcctatt attaggtgca
     3361 tatttttgtt gtttgctgga tgtaatccat agttatttat tgacattttt gttcatcctt
     3421 tttgctaaaa aaaacaataa cctatttttg taatgtaata gatattagtc tattgctaat
     3481 ctaaaaaaac cattgccaca ttgaatggct atattcacca ataaattctt tcgatttcgt
     3541 atattgacat atcgtagata tatcttatcc ctaagattca ataatcgagt ccgtttacca
     3601 ttctgtgcaa gcgccttcac tcagaagtga ccgtcaaggc gatcaccaaa cttgataata
     3661 aagcagctca ttgccatacg ccagtcctta aggggcgttg tccatttctg tgatgctgcc
     3721 tagatttaac tgcctgctcc ggcttatcta catctaaata tgtgagccta ataccgctac
     3781 actttggcca gactatgttt ttctccggga agtgggctaa ccgtctccat aaagtgacga
     3841 acttttttca acagtggcca tattgaacgg cactgatgat tacgggttat tgtctcatga
     3901 agagccggcc acatcactga cagcagtgac ttcaggcctt cagcaccaga cagcctgaac
     3961 cagttaatcc atcgcccgac agatttagta atctagggct ggtttttctt cagctgttgc
     4021 ggttcgaaag tgccattacg atcacgcaga gtacggattt ccagaggttc atctccagta
     4081 gtgataatct tcgttaagca accattgcgg gaattgctgc cagatctaag ctgatttttc
     4141 tcgcacccaa gatgatgagt catattagca ttgagtacgg tttcattttc agcatccaat
     4201 caaactgact gagatcttca ggatttttga gacttttggc tagttcatta gctaaagccc
     4261 gcaacttatt agatgtcaac tgtttttcat tcataaattc accttcattt gatatcaaat
     4321 taaatagttc aacatcagag aattatacaa acttatgtac aggctcctat gatcttcgaa
     4381 tcctgaagat tatagtgaaa ataagttttc acgttgaaat cccacatagt tagtatatga
     4441 taaataatca tggatgacct gattaacctc ttggggtagt gcttttatag aagaataaat
     4501 attctactaa tttaatacat cacctaaaat agtattgttt tttatggttg gttgaaggtc
     4561 gtggtgatga tgttagtcct tttgagaata ttccagtgcc agatcttact tataataaag
     4621 gggcttggca gtttggataa actattgttc ttattccact aatacaaact ttttgtaaag
     4681 tatattaaat atacttcatt tcatactgaa atccccggaa gtattcaatc aaacaattac
     4741 accataaaag gaagaggggg ccctaattaa gttgtcaagc atgtttatga tacctgcagg
     4801 gtataaattt tctggctgca agcagacaca tataaaacgt ctttattgat cctacccaca
     4861 taatatggac acagtcgtaa gtgaggctct ggttttcaaa ttgttccgga ctgagaccac
     4921 cacaccaacg gtgccgtcgc cagcgattgt aatcgcactc gatataacta aacactgttg
     4981 cccgcactat ttcccgatgg ataatagtgt tctccgtgga gacattccat tttcagcgag
     5041 tgaattttca tctgttgctg tatccagtca tacagtgact gcatcagtgg cgcggctctg
     5101 gcttttcttg ccgccagacg ctgttctgct gaacatcccc ggacttctgc ctcgatggca
     5161 tacagttcac cgatacgcag cagggcttcc gttattctgc cggattcgta caacgcccgg
     5221 taaccaccgt aagcatcggc ctgaagcact ccgctgtaac cggccagatg attctgtgga
     5281 tggatacctt tccggtccgg tgagtacgcg aaccagaccg ccggaggcat ttccgaaccg
     5341 gcgttacggt catcacggac atagacccac agccgggcag ttcttgtttt accactgccc
     5401 ggctcctgga ccggaacggg gatatcatcc gcatgaactt taccgggcat cagcacatac
     5461 tggcgcagga cgtcatacag cggctccagc agttcagcaa cggcaccggt ccagcgaccc
     5521 agtgtggcac ggctcagctc cacgccctga cgacggtata tttctgactg gcggtataac
     5581 ggcagatagc ctgcatattt tccagcgaca acatgggcca gaagccccgc tccggcataa
     5641 ctgcgtgcaa tgggttttga aggcaccggt gcctgcacga tatggtcaca ccggcaacag
     5701 gccagtttcg gacgttgtgt ttcgataacc ttaaaggcgc tgctgataag ctccagctgc
     5761 tctgacacat cacatcccag agaactgagt tcgccaccac aggccgggca gcattcctct
     5821 tccggacgga tgacccgggt ttcacgggga agtgaggcag gtaacggttt acgggctgaa
     5881 gactggcgca ggggagatgg cagtaccggg tcatattgct cacccagcgt ttccgccatt
     5941 tcttcctgaa gtgcgctgat tcgctcctgt gcttccagta tctgccgttc ggtttttgcg
     6001 cgaagttttt ctgagcttta accgaactgc atgcgctgca gtttcgcaac cagcgccttc
     6061 agccggttga tttcggaagc ataagccgcc acccgctgtg agagcaggcg gttgtatttc
     6121 gccatctggc ggatggtgtc ctgttgcgtc tgcaacagtg cccgcaggcg ggcattctca
     6181 tgagcaagtg aggtgtccat atcctcactt tacaacgggt tatatgcgga tgccagcgcg
     6241 ttccgttcgt ttcgggtgtt tccagtcgat gccctcaaga agcatggata actgggccgg
     6301 agtaaggtgc accttgccgt cacgggtgac cggccagacg aagcggcccc gctccaggcg
     6361 tctggtaaag aggcacagtc cgtcactgtc agcccacaac acttttatct ggtcaccccg
     6421 gcgtccgcgg aagatgaaca gatgcccgga gaacgggtca tccttcagga cgttctgaac
     6481 ttttgaggcc aggccgttaa agccatttcg catatcggtg atacctgcaa ccagccagat
     6541 acgcgaacct gcagggaaag atatcatcag tggctgctcc cttttatttc gcggataagt
     6601 atctgtaata acgccggcgt cagtttacct ttaagcctga gagttccggc cggcagaacc
     6661 agctcacagc acagactgtc ggacggtgta tttatctgct ctggttcctg tgccggagcc
     6721 gggattttat tatccggctc cggcgttaac gtcacgggaa gcagtgcagg catatttttt
     6781 ccggaaggca gcaggccacc tttccggtat tgatggcgcc agttgaagag caggttatcg
     6841 ttgattccgt tttcacgggc gatctgcgcc acacaggctc cgggctgcag tgactgctcc
     6901 actaaggcga ttttaaattc ataaggaaaa ttaggccgcc gg
//
LOCUS       DAJTOR010000195         6837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTOR010000195 DAJTOR010000000
VERSION     DAJTOR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.195"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..297
                     /locus_tag="OO249_003665"
     CDS             <1..297
                     /locus_tag="OO249_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5637805.1"
                     /translation="LPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVT
                     FANVFTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(448..3771)
                     /gene="mscM"
                     /locus_tag="OO249_003666"
     CDS             complement(448..3771)
                     /gene="mscM"
                     /locus_tag="OO249_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5637806.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(3793..4761)
                     /gene="psd"
                     /locus_tag="OO249_003667"
     CDS             complement(3793..4761)
                     /gene="psd"
                     /locus_tag="OO249_003667"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5637807.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(4858..5910)
                     /gene="rsgA"
                     /locus_tag="OO249_003668"
     CDS             complement(4858..5910)
                     /gene="rsgA"
                     /locus_tag="OO249_003668"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5637808.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6005..6550
                     /gene="orn"
                     /locus_tag="OO249_003669"
     CDS             6005..6550
                     /gene="orn"
                     /locus_tag="OO249_003669"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5637809.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDSFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            6761..6836
                     /locus_tag="OO249_003670"
     tRNA            6761..6836
                     /locus_tag="OO249_003670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6794..6796,aa:Gly,seq:gcc)
BASE COUNT         1577 a         1839 c         1838 g         1583 t
ORIGIN      
        1 cttccttacc tgttcctcgc gctggctttc ccattcttta aagcacgtca ggatctcgac
       61 agaccatttg tgattttcaa aacgcgtatg tcggcaatga ttgcgacggt ggttgtcgta
      121 ctggtggtga catttgcgaa cgtcttcacc attattccgc ctgtggttga agccggagac
      181 tgggacagca cattgtggat gattggcggc cctgtcttct tctcgctgtt agcgatggcg
      241 atttaccaga actattgcag tcgcatggcg aataaacctg agttagctct cgactgatca
      301 tatgaaatcc tgcaactcat cgtctctccg gcgatgagtt gtcaggaagt aatgcgtatg
      361 gatgatactc tccagggccg gtgataactg aagatgatgt taagctgcta aatgagccac
      421 gttgacatca tttggcagct tcatgcatta caaacttccc gcctgacgac ctttgcccgc
      481 agacgtcagc gttctccccg tttgtttacc gttgaggctt tccagacgca tctggaaggg
      541 cgggaatggc atatcgatac cgtgggcatg gaagccagcc agaatcagct ggtggatctc
      601 atggcgtagc ggcatacggt gacccatctc agcggcataa atacgcagct cgaaaatctg
      661 aatcccttgt tgcagatcca ccaggaagac ttccggtgcc gggttgtcga tcaccagcga
      721 gcagcgacgc gctgcggtga gcaggatttc cgtcacttct tcactgttgg catcagcagg
      781 ggccggaatc gtcaacacta cgcgcgtaac cgagtcagag agcgaccagt tgataaactg
      841 ctcggtaata aacgccttgt tcggcacgat tatctcttta cggtcccagt cgctgatggt
      901 ggtggcgcgg gtgttaattt tcgtcacgct accggtgaga tcgcgaattg tcaccgtatc
      961 gccaatgcga atcggttttt cgaacaggat aatcagacca gagataaagt tggcgaaaat
     1021 ttcctgcaaa ccaaaaccga gaccaacacc gagcgcggcg accagccact gcaatttcga
     1081 ccattcaata ccaatcatcg agaagccgac cagcccgcca atcagcatca gcagatattt
     1141 ggtgatggtg gtgatggcgt agcctgtacc cggcgttaaa tccaggtgct gcaaaatcgc
     1201 cagttccagc agcgcgggca ggttgcgcac cagctgcgtg gtgatgataa acaccagaat
     1261 ggcaatcagc accgcaccga gggtaattgg ctccagactt tctacgcctt gtaccgtgga
     1321 ggtgacatcc cacagcgaaa tattttcgag gaagccgaaa gcggaatgga tttctgacca
     1381 cagcacaatg acagaaagca gggcgatcaa catcaaaatt gagcgcacca gccgcaagga
     1441 ttgcgcactg atggcatcga gatcgacttc gctttcatcg acttcaattg ctccttccgg
     1501 gctactgtga tgatgcgctt cctcttcgcc acgcgcacgt tgcgctaaca tctctgcccg
     1561 gcgatgcttc gcccggtcaa acgccagcct gcggcgctgg atcagcatcc agcggcggat
     1621 aacgtgataa accaccagta gcaggaacca gatggcaacc gaggtttcaa gcctcgctaa
     1681 cagcgcctgt gccgttgcca gataacccac agccgacgcc agaatggcaa ccaacggcgc
     1741 gccaatcatc atgttccaca gcatatggtt ggtaatgttg tcgccgctgc cctctttgtt
     1801 gagatacagt gggatcccgg cctttttcag gctgagggtg accaccgcca gcgcaccgca
     1861 aatgaggata aagcaaagcc gtcccagcga accggagaat tcacggtcgt cgaggttatc
     1921 gaacatcatc agcgccataa tcagcggcac aataagcccg atgctcatca ggtagtagcg
     1981 catcccacgg gaaacacgtt cgcgcggcca gccaaaatga gcgataaaca agccgttcgg
     2041 gcgggcaaag gtggcgcaaa tcatcactac ccacagcagc ggcacggtag ccgttacacc
     2101 atcgccaatc gcgaccgcca gcggataagg ccacgcctcg cgcaagccgt aacccagcgt
     2161 catccacagc accggtaacg gtgacgcgac gagaatcgac cagaaaagag tgcgcaacgt
     2221 cagccagaag tgatcctgag tcactttgcc aactttcgcc gccgaacgtt caagaaaacg
     2281 ggtgaaatag cggcgcgagt aaatactgca accgaccaga atcaacgcgc caaacagcgg
     2341 caaaattgtc tctttgctgg tcagcatcat cacactggct ttgcccaact gactgaaggt
     2401 gtccagcgaa atgagacgac gcagatcctg ggcgatttcc agcggccagg cgatggtcat
     2461 cgggcgcacg tcagaggtcc agaacagata gcggtgcgtt gcttcgttca cctctttcag
     2521 cgcatcctcc agttgcccgt tggagacttt cagcttggtc agttccaata gtagcgtgtc
     2581 gccaccctgc aacaatgagt tcagcaactc acgctgagtg cgcagttgtg cttccagaat
     2641 acggttttgc tcggcggtca gcggctgacc gtcggcctga tggatttgcc gtagcagcgg
     2701 ctgtttatta agcagatcct cataacgtaa ccgttgcaca cgcaactgcg ccatttcggt
     2761 atcaagctgt tgtggttttg gcatttctgg cagccgtgcc acctgcgccc gcagcgcttc
     2821 gccgagcaga ttggacgatc ccagccattg cgactgttca cgcagcgtat tcaacgcctg
     2881 ccggacctgt aacgtctggc tggcagcctg acgctgttgc gaggcaacga gatccatccg
     2941 ctgcgcctgt tgattcaaag ccgccgatag ttcgcggtta attttgaatt gcgcgacgat
     3001 atctttcggc aaatcggcgc tgttttctgc cagcaattcg gtactttcca gcgcccgctc
     3061 cgcctcaagc tgacgttggc tgtttaattg attacgcaag gcctgcaaat acgcatccag
     3121 ttgctggctc tctttttccg ccagctctga gcgtaagcgc gctaattcct ggcggttatt
     3181 ggcagacagc tgcgccagct ccagttcatc aacgagcgcc ttaagacgtg cagagtcaga
     3241 ctgcaacgcg aaattttgtg cctgattgag cggagtattg ccggtaagcg ttcccaggcg
     3301 gcgctcgatc tcatttaact gacggcgggc gtcggtttgc tgttgcggca gttgattcag
     3361 cgaatcggca atctcgcggg tgcgctcctg ctcttgctgg gcctgacggc ttttatccag
     3421 caactggctg ctgacctgga gaatttcctg attcagcgcg tcggtagaca ttcccggcga
     3481 cacgctgcgc ggctcgtcac gcatgttgtt taattgtgcg cgcagagtag cggagagttt
     3541 cggataatta tcgataactt gctgatattg tttgatgcgc tcaagggaac cttttcgttc
     3601 ctcaagcgca tttaaggcag actggagcgc ctctacgact tccggctgtg cgggtttcgc
     3661 cgcttttgcc tgctccagtt cctgagtgat ttgtttgcta tcgggggccg tcgcggcgta
     3721 cgccccccaa ctgaggcacc aggccatcag aaaagtgata atcaggcgca cgtcagcgtt
     3781 tcctttgatg gattagacct ggtctttttt atcgtcaacc aatgggctgg cgtcgtgttc
     3841 tgcttcgatc tcttcagcag gaagtggggc gggttcagcg tctggcgtaa caaaggtttc
     3901 ggtagatact gccagcggct ggccaatttt cgtgacagac aggctttcca gttgctcaac
     3961 cagattcact ttacccggtg caaacaggtt gataacggtg gaaccgagtt taaagcgacc
     4021 catttcctgg cctttcagca gtgccacaga accgtcgttt tccccggcag gccaggtcca
     4081 gcgcttgatg ataccttcgc gcggcggcgt aatggtgccc gcccagaccg tctcaatgct
     4141 gccaacaatc gtcgctccga ccagaatctg cgccattggg ccaaattcgg tatcgaaaag
     4201 gcaaatcacg cgttcgttac gggcaaacag attcggcacg ttctgagcgg tgagatggtt
     4261 aacggagaag agatcgcccg gcacgtagat catctcacgc agaataccgt tgcacggcat
     4321 gtgtacgcga tggtagtcac gcggggagag gtaagtggtc acaaacgtac cgttgcggaa
     4381 caggtccgcc atcagatagt tgcctgccag cagggcttcg aggctgtagt tgtggccttt
     4441 ggcttgcagg attttatctt cttcgatttt acccaactgg ctgataacgc catcggcagg
     4501 catgaccagt acattcggat cggtatcgat tgggcgtact tcgtcacgca gcggacggac
     4561 aaagaattcg ttaaaggtgc ggtagctggc ggtgtccggc ttttgcgcct ctttcatgtc
     4621 gaccttgtag tatttaacga acagatcgat aaccagtttt gtcagccatc ctgcccgctt
     4681 gcttgcgccc caacccgcca ggcgagtaag ccatagtttc ggcagaatgt actgtagcga
     4741 aagtttaaat gaatttaaca aggtagcctc caggccattg ttttgtcgtt cctgatccgg
     4801 ccaacatgcc ggatcctgaa aaaaagggga cgattctaac gacggttagc ttaattgtca
     4861 gtcatccgta tcagaaaagt ttttacgcgt ttttacctgc gccatgcttt ccagaatacg
     4921 gtgatagttt tcgaaacggg tttccgcgat tttcccttcc tcaaccgctt cccggatagc
     4981 gcagcccgga tcggtatcgt gtttgcaatc gcgatattta cacagaccta aatagtcatg
     5041 gaattcgaca aagccctgag tgatttgttc cggctccagg tgccagaggc cgaactcacg
     5101 cacaccaggg gaatcaatca catcaccgcc gtgcgggaag tgatacagcc gagcggcggt
     5161 cgtggtgtgc tgaccgagcc ccgagttgtc agagacatcg ttggtcagga tctctttttg
     5221 cagccccagc aacgcattca gcaggctgga tttaccgacg ccagactgcc cggcaaaaat
     5281 gctgatgcgc ccggtcaacg cctcttccag cggttttagc ccatcctgag tatggctgga
     5341 aaccatcaat acgcgataac cgatattgcg gtagatatcc atctgctcgt tgacgaacgc
     5401 catgccttcg tcgtccagca ggtctatctt gttgagcaca ataatcggct caatctgcaa
     5461 ggtttcgcag gccaccaggt aacggtcgat aatattgagt gacagctccg gcaaaatggc
     5521 ggagacaatg acaatctggt cgatgttggc ggcaataggt tttacgccgt cgtagaaatc
     5581 cggacgcgtc aacaccgagg tacgctcatg caccgcttcc acgatccctt tgacatttac
     5641 gccttccgcc gccggtttac ccggacgcca gactacacgg tcgccggtca ccagcgaacg
     5701 gatggtacgg cgaatattgc agcggtgaac gtcgccatcg gcggattcca catcagcgtg
     5761 cataccaaag cggctgatga cgataccttc atcaggctcg ccaaacagat tgtcgtcgta
     5821 gtcgggcttc tccttagacg ttttaagacg acgctggtga ttggcgttca cgcggcgctg
     5881 ctggcctttg gagagtttat ttttactcaa tcgtacagac tcctggtcgc ccctgatggg
     5941 caaaacatct atgatacacg caattgtgga tcaatatagt cactgtgaat gggtggaaaa
     6001 tagcatgagt gccaatgaaa acaacctgat ttggatcgat cttgagatga ctggtctgga
     6061 tcccgagcgc gatcgcatta ttgagattgc cacgctggtg accgatgcca acctgaatat
     6121 tctggcagaa gggccgacca ttgcagtaca tcagtccgat gaacagctgg cgctgatgga
     6181 tgactggaac gtgcgcaccc ataccgccag cgggctggta gagcgcgtga aagcgagcac
     6241 gatgggcgat cgggaagctg aactggcaac gctcgaattc ttaaaacagt gggtgcctgc
     6301 gggaaaatcg ccgatttgcg gtaacagcat cggacaggac cgtcgtttcc tgtttaaata
     6361 catgccggag ctggaagcct acttccacta ccgttatctc gatgtcagca ccctgaaaga
     6421 gctggcgcgc cgctggaagc cggaaattct ggatagtttt accaagcagg ggacgcatca
     6481 ggcgatggat gatatccgtg aatcggtggc ggagctggct tactaccgcg agcattttat
     6541 caagctgtaa aatttttcct ggtcacgtaa gcgccggaat ccacttgcgg cggcgtgatt
     6601 ttgacgctaa attgaacgtc ttgctgataa tttcagcact tgagataaaa acgcaaaaaa
     6661 aacttttttg gggggttgca gagggaaaga tttctcgtat aatgcgcctc ccgtaacgac
     6721 gcagaaatgc gaaaattacg aaagcaaaat taagtagtac gcgggaatag ctcagttggt
     6781 agagcacgac cttgccaagg tcggggtcgc gagttcgagt ctcgtttccc gctccaa
//
LOCUS       DAJTOR010000196         6803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTOR010000196 DAJTOR010000000
VERSION     DAJTOR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.196"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            176..904
                     /gene="mpaA"
                     /locus_tag="OO249_003671"
     CDS             176..904
                     /gene="mpaA"
                     /locus_tag="OO249_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5637810.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPSSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(879..1844)
                     /gene="ycjG"
                     /locus_tag="OO249_003672"
                     /pseudo
     CDS             complement(879..1844)
                     /gene="ycjG"
                     /locus_tag="OO249_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
     gene            1963..2469
                     /gene="tpx"
                     /locus_tag="OO249_003673"
     CDS             1963..2469
                     /gene="tpx"
                     /locus_tag="OO249_003673"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5637811.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2513..4054)
                     /gene="tyrR"
                     /locus_tag="OO249_003674"
     CDS             complement(2513..4054)
                     /gene="tyrR"
                     /locus_tag="OO249_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5637812.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4202..5263)
                     /locus_tag="OO249_003675"
     CDS             complement(4202..5263)
                     /locus_tag="OO249_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5637813.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREICRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5260..6657)
                     /locus_tag="OO249_003676"
     CDS             complement(5260..6657)
                     /locus_tag="OO249_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5637814.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
BASE COUNT         1625 a         1936 c         1662 g         1580 t
ORIGIN      
        1 tacgacttac atctaagtca gcataattct aatatctgca catcaataaa aatgcaaaca
       61 acttccggca aaaaaagtga tacaagatcg atgacatagc gttgaagaaa aggatttgat
      121 aatccgcaaa atttgcacaa cactgcctgt aatacttcct gaaccagaga cgatcatgac
      181 cgtaacccgc ccacgcgccg aacgcggcgc atttccgccc ggaacagaac attacggacg
      241 ttcattattg ggcgcgccgt taatctggtt tcctgcccct gcagccagcc gcgaaagtgg
      301 tttgattctg gctggcactc acggtgatga aaactcttcg gtcgtcacgc tctcctgtgc
      361 tctgcggacg ttgacacctt ctttgcgccg ccatcatgtg gtgctgtgtg tgaatcctga
      421 cggctgccag ctggggttac gggccaatgc taatggtgtg gatttaaaca gaaactttcc
      481 ggcggcgaac tggaaggaag gtgaaacggt ttatcgctgg aacagcgccg ctgaagaacg
      541 tgatgtcgta ttactgacag gtgacaaacc tggctcagaa cctgaaaccc aggcgctgtg
      601 ccagcttata caccgcattc aacctgcctg ggtggtctca ttccacgatc cactggcctg
      661 tattgaagat cccagacaca gcgaattagg cgaatggctg gcccaggcgt ttgaattgcc
      721 tctggtaacc agcgtcggtt atgaaacgcc tagctctttc ggcagttggt gtgcagatct
      781 caacttgcat tgcatcaccg cagagtttcc gcccatctcc tccgacgaag ccagcgaaaa
      841 atatctcttt gcgatggcaa acttgctgcg ctggcatcct aaagatgcaa ttcgcccgtc
      901 gtgaactgaa gcgccggttc cacatctacc gccagccagg tcggtccgtc aagatcggcg
      961 aaactgacct gcggcaccag cggtaaagcg gcgctaatgg cacgagaggt acacaacatg
     1021 cagcccagca tcagactgaa accttgtgca cgcgcttcag tcgccagcgc cagcgcttcc
     1081 gtcagaccac cggttttatc gagcttaatg ttaaccatct catagcgccc tttcagcgcc
     1141 ttcagattgc tacgtgtatg acaactctca tcagcacaaa tcggcaacgg atgaataaaa
     1201 ttctccagcg ccgcatcgtc ctgcgcagga aggggttgtt caagcatcgc aacgcctaaa
     1261 tccgccaata gctggcaacg cgccgccaac ccttatgcac gccaggattc atttgcatca
     1321 acgatcagcg tcgcatcggg cacagctgtg cgaattgcca ccatccgctc actaataaga
     1381 tggttatcca gcttcacttt cagtaatttc gcgcctgcct gccagagtgt tgatgcacta
     1441 ttggccatct gatcaggcgt accgatgact accgtctgtg cagtgataac tgtctcggga
     1501 agcgttatgc cgatcaaatc agccagcgat tgctgctgtt ttcgcgcggc cagatcccac
     1561 aatgcacaat ccagcgcatt acgtgctgcg ccggcaggga gaattttttg caactcctcc
     1621 cgtgtcagcc ctttctctaa ttgcgacacg acgctcataa tttgcgccat taccgaggca
     1681 tcactttctc cataacgcgg atacggcgtg cattcgccgg tgcctttaat accctcttct
     1741 tccagttcaa ccaccaccac gcgcgcttca ctgcgacttc cccgggcaat cacaaacggg
     1801 gtatgtaagg gctaggcttc ctcaaatacc ttaacggttc tcatctttac tccttgttgt
     1861 cgcggtggtc tgcggattaa gcgcaaatag cgtttgctgt gttattgaca gttagcataa
     1921 actaggtgtg acgttaacta tatgtaaaca ggaaagataa tcatgtcaca aaccgttcat
     1981 ttccagggca acccggttac agtcgccaat tccatcccgc aggcgggtag caaagcgcag
     2041 acttttactc tcgtggcaaa agatctgtct gacgtcaccc tcggtcagtt tgcgggtaaa
     2101 cgcaaagtgc tgaacatttt cccgagtatt gataccggtg tttgcgccgc atcagtacgt
     2161 aagtttaacc aactggcaac cgagatcgac aacaccgttg tgctgtgtat ttctgccgat
     2221 ctgccgttcg cccagtctcg tttctgcggc gcagaaggtc tgaacaacgt tatcaccctc
     2281 tccactttcc gtaacgctga atttctgcaa gccttcggtg tggcaattgc tgatggccca
     2341 ctgaaaggtc tggcagcgcg tgccgttgtg gttattgacg aaaatgacaa tgtgattttc
     2401 agccagctgg tggatgaaat caccaccgag ccggattacg aagcagctct ggctgtactg
     2461 aaagcataag ttaatatgcc tgatggtgtt gcaccatcag gcatattcgc gcttactctt
     2521 cgttcttctt ctggcttaag ccatattccc gcaacttatt ggcaatcgcg gtatgtgaaa
     2581 cgccgagacg ttttgccagt ttgcgcgtgc tgggataatt gcgataaagc tgggttaata
     2641 ccgagcgttc aaaacggctg gtgatttcgt ccagcgaacc ttccatcgca tcttcgccca
     2701 cggctaccgt tgcggcgtca taatccggca acaaaatatc ctgtggacgc agctcataac
     2761 cgtccagttg tgtcagtgcg cgatatatag cgttctttaa ctgccgcaca tttcccggcc
     2821 acgcataacg cgtaagtaca gtattcaggt cagcggccag tttcggacgc ggcacgccct
     2881 gctcgtcggc aaagcgggcg acgaacagct cagttaacgg catgatgtcc tgcggacagt
     2941 cacgtagcgg cggcagattg agcgtcaaca cgttcagacg ataatagaga tcttcacgga
     3001 acatgccttt ttgcaccagt tcgaccagat tcttctgcgt agcgcaaatc acccgcacat
     3061 cgacatgcac ctcatggtct tcgccaaccc gacggaaagt gccatcatta agaaaacgca
     3121 gtaatttcgc ctgcatccgt ggtgacattt cccctatttc atccaacagc accgaaccac
     3181 cgttcgcctg ctcaaagaat cctttcttcc cttccggagc atgaccaaac agttcactct
     3241 cgaccgcatc ttccggtata gacgcacagt tcagcgccag gtaaggtttg cccgctctgg
     3301 ggcttgcctg atggcaggcg taggcaaaga gatctttacc tgtacctgtg tcacccgtaa
     3361 tcagcagcgg cgcgcttagc atcgccagtt tctgcgcctg ttcgacaaca tgcttcattt
     3421 tcgggctgac ggcgacaatt tgactgaagg cgctgacgtc ctgggcggcg acattttgca
     3481 actggcggcc catacgaatc gttgatcgca acatcaccac cgcaccggtc aggacgtgtt
     3541 gatcattttc atcctgaaga taaacaggcg taatctccat caggaaattc tgcccattaa
     3601 taacgacatg ctcgttatgc gaatcttgcg gttcgctttc cagccaacgt aaaaaattaa
     3661 agccgttaat caattgtgcg gcggtatggt tgcgcaggcg atccaatttt tgcccaaaaa
     3721 gctgacagct cgccgggttc gccatatcca ctttgctttt catatcgaca gagagcacag
     3781 gttcgggcaa cgcctccagt aacgcgctca acgccagatg ctcacgttcg gaaggcatcc
     3841 acgggacagt acgcacatcg gtaacacctg caatacggcg tatttcggcc atcagactgc
     3901 tgaaactctc aaactccagt tcagcaaaat tgaggtagat tcgcccaatg ggatcaatct
     3961 caataccgcg taaatcaatg cctcttagca cgagtagatc gagtaattcg cgggtcagac
     4021 cgagtcggtc ttcacaaaag acttccagac gcatgggaac cttcacctga aaaaagaaca
     4081 attaatatga tgatatgaca ctatttggat agcaggaagg tttctgtcaa caatcattga
     4141 cacaaagacg gaaaaacggg cacttcggcg taaagatatc cggtcagcac ccgatattgc
     4201 attatttttc gctgggcgtt ttgccttttt gcagcgtttc tttcacctga ccgataagct
     4261 gacgacggaa atccccgagg cgaggtttgt catcgtcaat ccacggcagc gggcggcaaa
     4321 gctccatagc tttaatcccg agtcgtgccg tcagaagtcc agcaccaatt ccctgagctg
     4381 cgcgggtaga caaacgagca gcgagatctt gcgacatcca gtccatcccc acttcgcgca
     4441 ccagttcgct ggctccggca aaagcgatat tcagcaatac cagcttaaac agacgcaaac
     4501 ggctgtaata ccccagttca atgccataca gcgtggcgat gcgattaatt aaacgcagat
     4561 tgcgccaggc gataaacgcc atatcgacca acgccagcgg gctgaccgca atcatcaacg
     4621 ttgattccgc cgccgaacgg cagatttcgc gccgcgcctg ggcatctaaa actggctgga
     4681 ccaaatgcgc atacaaactg accacttcac ggtcgttttg cgtttcatgg attgaggcat
     4741 accagcgttg cagcgctgga tgagactgat caatacccgc ctgctgcgcc agtttttcgc
     4801 aaaatgcgcg gcctttgccc gtgccgtggc tgtgcaacaa atcgcgcgct tcgtcgcgtt
     4861 catgggcgcg ctgtcgcaag cgccataagc gccgccactc tgttaccaca gaacctacgc
     4921 cagcgccgat aatcaatgcc ccagcggcac atccacccag cgccacccag tcctgagttt
     4981 gccaggcatt cattgtccac tgtacaccct gcccgacaac gcttgcgcca aacagagcca
     5041 gcccgcccat caccattttg cgccacaggc tgcgtttcgg acgtaacgct gcatccatta
     5101 ccgcttcaac ttgcccctct tcttcaggcg cttcatcgag cgtggccggg gcaaaatttt
     5161 gcgcctgatt ttcgtcaaag gtctgctgcg ccctgaattt tggattctga tcgacctcca
     5221 gcggaccgtc gaaatcaata cgtggtttta acggttcggt catcgcaatt tatctcctat
     5281 taaaaattcc agcgcggcat caagacgaat atgcggcagc ggtttgtcga catccatcac
     5341 ctgcgggcga aacgcttcaa actggaaccc ttgtttgtcc cagaacgcct gaccgggcaa
     5401 acgcgcagga acttcaccgg gataaacggt gagtggcgca ccatcgctga gccggttacc
     5461 gcgcaatgcc ggaattttct cgccgttgac gtcaataatg ccgctggtgg tcgcctgcac
     5521 cgaggccagc cccaggcaat ccatactaat cccttcaaac gccgcatttt gccaggcatc
     5581 ctgaatcagt tgttgcagta atgaaaccat attggcgtgc tgatcgatgg tcacatggtc
     5641 cgctttagtg gcagcaaaca atagcttatc gataaccggc gaaaacaaac gccggaacaa
     5701 ggtacgctgc ccgtagtgga aactttgcat cagctgcgtc agtgccagac gcatatcatt
     5761 aaatgcctgt ggcccactgt tgagaggttg caggcaatca accagcacaa tctggcggtc
     5821 aaagcgcaga aaatgattct tatagaaccc cttcaccacc ttctcgcagt aataattaaa
     5881 ccgctcgcgc agcattccgg cattggtgtg cttatcggcc tgcgccagtt tggactcgcc
     5941 ccaggtatcg acatccggcc acgggaagaa ttgcagcgcg ggcgcacctg ccatatctcc
     6001 tggcaagaca aagcgccctg gctgaataaa gtgcagcccc tgctgtttac agtggtggag
     6061 ataatcggtc cacgcggcgg caatgtctgc cagccggttt tcgtcggcag gtgctagcgg
     6121 gtccagccct tcgcacatca ttcgccattt gaccgaccat tcgccgcgct gaccattgag
     6181 taagcccgtc atctggcgtg accagcttaa atagtcctgc gccagcatcg gcaggtcgag
     6241 caaccattcg ccgggataat ccacaatctc cagatacagc gtggaggtgt ccttgaagtg
     6301 gcgtagcagc gaatcgttcg atttaaaacg tagcgccagg cggatttcac tgacgccgcg
     6361 cgttggcgtc ggccaggcgg gaggatcgcc atataactgc gccagtcctt catcatatgt
     6421 gaagcgcgga atgccaaagt cacgctgagg aatgcgtttt acgcccagca ggcgctcttc
     6481 acgcaccgcg cttaacagcg gcaagcgtgc tccggcgtga atattgagca actgattgac
     6541 catagcagtg ataaacgctg ttttgccgct gcggcttagt ccggttacgg cgaggcgcag
     6601 atgtctgtcg acaccccgat tcaccagcgc attaagttca tttttaagtc gcttcattgc
     6661 tgtcctgtgc tgctctgaag accagaaaaa aatcattcgt aaggatatga atgtgggggc
     6721 caggccattg attatcaact gcccgtgttg tcatcgacgg gcgaactcca taagatgtgc
     6781 agcattcacg ttcggacgga gtt
//
LOCUS       DAJTOR010000197         6800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTOR010000197 DAJTOR010000000
VERSION     DAJTOR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.197"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            41..247
                     /locus_tag="OO249_003677"
                     /pseudo
     CDS             41..247
                     /locus_tag="OO249_003677"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            992..2905
                     /locus_tag="OO249_003678"
                     /pseudo
     CDS             992..2905
                     /locus_tag="OO249_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3033..3755)
                     /locus_tag="OO249_003679"
     CDS             complement(3033..3755)
                     /locus_tag="OO249_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5637815.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            3902..6529
                     /gene="gyrA"
                     /locus_tag="OO249_003680"
     CDS             3902..6529
                     /gene="gyrA"
                     /locus_tag="OO249_003680"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5637816.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDAAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
BASE COUNT         1633 a         1672 c         1965 g         1530 t
ORIGIN      
        1 aggctatgga gatggtcctt ccactgcggc gggtggcttt atgtatctcg gcttaagtga
       61 agttaccttt gatattgccg acggaaaaac gctggttatc ggcaatacag agaatgacgg
      121 agctgttgac tctattgctg gtaccgggtt gatcaccaaa acaggttccg gcgatctggt
      181 acttaatgca gataacaatg actttactgg cgagatgcag attgaaaacg gtgaagttac
      241 cctgggccgc agcaactccc tgatgaatgt cggcgatacg cattgccagg acgatccgca
      301 agactgctac ggtctgacga tagggagtat tgataagtac cagaatcagg cagagctaaa
      361 cgttggctcc acccaacaaa cctttgtgca ctcattgacg ggctttcaga atggcacttt
      421 aaatatcgat gctggtggca atgttactgt taatcaaggt agttttgctg gcaccatcga
      481 aggtgctggt cagctcacca ttgcgcaaaa cggcagctac gtgctgtcgg gggcgcagtc
      541 gatggcgcta accggcgata tagtcgttga tgatggtgcg gtgctttcgc tggaaggcga
      601 cgcggcagat ctcgccgctc tccaggacga tccgcagtcg atcgtgttaa acggcggtgt
      661 gctcgatctc tctgatttct ccacctggca gagcggcaca tcatacaatg atggccttga
      721 agtcagtggc agcagcggaa cggttatcgg cagtcaggat gtggtagatc ttgcaggtgg
      781 cgacaatttg catatccgcg gcgacgggaa agatggcgtc tacgtggtgg tcgatgcgag
      841 cgacgggcag gtaagtctgg caaacaataa tagttatttg ggcacaacac aaatcgcctc
      901 cggtacgctg atggtgagcg acaactcgca gcttggagat acccactata accgccaggt
      961 tatctttacc gataagcaac aagaaagcgt gatggagatt actgccaatg tcgatactcg
     1021 ctctacgacg actgagcatg ggcgtgatat tgaaatgcgc gccgacggtg aagtggcagt
     1081 tgatgcgggg gtagacacgc agtggggcgc actgatggct gacagcagcg ggcagcatca
     1141 ggatgagggt agcacattta ctaaaacggg ggcgggtacg ctggagctga ccgccagcgg
     1201 tacaacgcag tcggcagtac gtgtcgaaga aggcaccctg aaaggcgatg ttgcggatat
     1261 ctttccttat gcttcgtcac tgtgggtcgg tgatggggcg acgttcgtta ctggcgcgga
     1321 tcaggatatt cagtctatcg atgctacttc cagcggcact atcgatatca gcgatggtac
     1381 ggttttgcgt ctgaccgggc aggatacttc cgtcgccctt aatgcctcat tgtttaacgg
     1441 cgatgggacg ctggtgaatg ctaccgatgg cgtgacgttg acaggtgagc ttaataccaa
     1501 ccttgaaact gacagcctga cctatcttgc cgacgtgacg gttaatggtg atctgaccaa
     1561 tacgtccggt gcggttagcc tgcaaaatgg cgtcgctggc gatacgctaa cggtaaacgg
     1621 tgattatact ggcggcggta cgctactgtt cgacagcgaa ttaaacggcg atgactcggt
     1681 aagcgatcaa ttggtgatga acggtaatac tgctggcaac acaactgtgg tggttaactc
     1741 cattacaggg attggtgagc cgacatcgac aggcattaaa gtggttgatt tcgcagctga
     1801 tcccacgcag tttcaaaaca atgcacagtt cagtctggct ggcagtggct acgtcaacat
     1861 gggagcgtat gactacacgc tagtggaaga taacaacgac tggtatctgc gatcgcaaga
     1921 agtaacgccg ccatcgccac ctgatccaga cccggaccca acacccgatc ctgacccaac
     1981 acccgatcca atccccgctt accagccggt gctgaatgcc aaagttggcg gttatctcaa
     2041 taacctgcgg gcggcaaatc aggcgtttat gatggagcga cgcgatcacg ctggtggcga
     2101 tggtcagacg ctgaatttac gtgttatcgg cggacgttat cattacacgg cggcagggca
     2161 actggctcag caggaagaca cttctacggt gcagcttagc ggcggcttgt ttagcgggcg
     2221 ctggggcacg gatggcgagt ggatgcttgg ggcggttggt ggctacagtg ataatcaggg
     2281 cgacagccgc tcgaatatga ccggaactcg cgccgataac cagaaccacg gttatgccgt
     2341 tgggctgacc tcaagctggt atcagcacgg taattagaag cagggggcct ggctggatag
     2401 ctggctgcaa tacgcgtggt ttaacaatga tgtttccgaa catgaagatg gcgtggatcg
     2461 ttaccattcg tcggggatta tcgcctcgct ggaagccggg tatcagtggt taccggggcg
     2521 tggtgtggtg attgaaccgc aggcgcaggt gatttatcag gacgtgcagc aggatgattt
     2581 taccgccgct aaccatgcgc gcgtgtcaca atcgcagggg gatgatattc agacgcggct
     2641 gggtttacac agcgaatggc gtaccgcggt tggtgtcaca ccaacattag atctgaatta
     2701 ttatcacgat ccccatgcga cggaaattga agaagatggc agcactatca gtgacgatgc
     2761 ggtgaagcga cggggtgaaa taaaagtggg agtcacgggc aatatcagtc agcgagtgtc
     2821 cctgcgcggc agcgtggcgt ggcagaaagg gagtgatgat tttgcccaga cggcagggtt
     2881 tttgtcgatg acggtgaaat ggtaatcact gtctagtcgg cacggttgcc ggatgcggcg
     2941 taaacgcctt atccggccta cggggagcat ttgtaggcct gataagacgc ggcaagcgtc
     3001 gcatcaggca ttggctcggc caaacaacaa cctcacttat tctgcgtgtg cagcatatag
     3061 ttcacatcca cgccggggcc gagtttaaaa gtattagtga gcgggttgta atgtagccca
     3121 gttatatgtc gctctttaag gcttgtctga tctacccagc caagcagttc tgccggttta
     3181 ataaacttct tcacatcatg cgtgcctttg ggcaccatgc gcaaaatata ttccgcgcca
     3241 accaccgcca tcagccatga cttgccgttg cggttaagcg tcgagaaaaa gacatcgccg
     3301 cctggtttca ccagttgcgc acaggctctg accactgact gcggatcggg gacgtgctcc
     3361 agcatctcca tgcaggtcac cacatcatac tgcccggcat gttttgccgc gtgctcttcc
     3421 acggtttcct gcacataatc cacctgaatg ccgctttcca gcgcgtgcag ttttgccacc
     3481 tgcaatggct caaagcccat atccagaccg gtcaccgtcg cgccttcgcg cgccatactc
     3541 tcggccagaa tgccgccgcc acaaccgaca tcgagcacct ttttgccaaa taaaccgcca
     3601 gcacgctcgg caatatagcc cagacgcagc gggttaatgc ggtgcaacgg tttgaactca
     3661 ccttcaagat cccaccagcg ggaggcgacg gcttcaaatt tagcgatctc ttcgtggtct
     3721 acgttatggt tttccggcga tttttcggca ttcattggca cttctactcc gtaattggca
     3781 agacaaacga gtatatcagg cattggatgt gaataaagcg tataggttta cctcaaactg
     3841 cgcggctgtg ttataatttg cgacctttga atccgggata cagtagaggg atagcggtta
     3901 gatgagcgac cttgcgagag aaattacacc ggtcaacatt gaggaagagc tgaagagctc
     3961 ctatctggat tatgcgatgt cggtcattgt tggccgtgcg ctgccagatg tccgagatgg
     4021 cctgaagccg gtacaccgtc gcgtacttta cgccatgaac gtactaggca atgactggaa
     4081 caaagcctat aaaaaatctg cccgtgtcgt tggtgacgta atcggtaaat accatcccca
     4141 tggtgacgcg gcggtttatg acacgatcgt ccgtatggcg cagccattct cgctgcgtta
     4201 catgctggta gacggtcagg gtaacttcgg ttccatcgac ggcgactctg cggcggcaat
     4261 gcgttatacg gaaatccgtc tggcgaaaat tgcccatgaa ctgatggccg atctcgaaaa
     4321 agagacggtc gatttcgttg ataactatga cggcacggaa aaaattcccg acgtcatgcc
     4381 aaccaaaatt cctaacctgc tggtgaacgg ttcttccggt atcgccgtag gtatggcaac
     4441 caacatcccg ccgcacaacc tgacggaagt catcaacggt tgtctggcgt atatcgatga
     4501 tgaagacatc agcattgaag ggctgatgga acacatcccg gggccggact tcccgacggc
     4561 ggcaatcatt aacggtcgtc gcggtattga agaagcttac cgtaccggtc gcggcaaggt
     4621 gtatatccgc gctcgcgcag aagtggaagt tgacgccaaa accggtcgtg aaaccattat
     4681 cgtccacgaa attccgtatc aggtaaacaa agcgcgcctg atcgagaaga ttgcggaact
     4741 ggtaaaagaa aaacgcgtgg aaggcatcag cgcgctgcgt gacgagtctg acaaagacgg
     4801 tatgcgcatc gtgattgaag tgaaacgcga tgcggtcggt gaagttgtgc tcaacaacct
     4861 ctactcccag acccagttgc aggtttcttt cggtatcaac atggtggcat tgcaccatgg
     4921 tcagccgaag atcatgaacc tgaaagacat catcgcggcg tttgttcgtc accgccgtga
     4981 agtggtgacc cgtcgtacta ttttcgaact gcgtaaagct cgcgatcgtg ctcatatcct
     5041 tgaagcatta gccgtggcgc tggcgaacat cgacccgatc atcgaactga tccgtcatgc
     5101 gccgacgcct gcagaagcga aaactgcgct ggttgctaat ccgtggcagc tgggcaacgt
     5161 tgccgcgatg ctcgaacgtg ctggcgacga tgctgcgcgt ccggaatggc tggagccaga
     5221 gttcggcgtg cgtgatggtc tgtactacct gaccgaacag caagctcagg cgattctgga
     5281 tctgcgtttg cagaaactga ccggccttga gcacgaaaaa ctgctcgacg aatacaaaga
     5341 gctgctggat cagatcgcgg aactgttgcg tattcttggt agcgccgatc gtttgatgga
     5401 agtgatccgt gaagagctgg agctggttcg tgaacagttc ggtgacaaac gtcgtactga
     5461 aatcaccgcc aacagcgcag acatcaacct ggaagatctg atcactcagg aagatgtggt
     5521 agtgacgctc tctcaccagg gctacgttaa gtatcagccg ctttctgaat acgaagcgca
     5581 gcgtcgtggc gggaaaggta aatctgccgc acgtattaaa gaagaagact ttatcgaccg
     5641 actgctggtg gcgaacactc acgaccatat tctgtgcttc tccagccgtg gtcgcgtcta
     5701 ttcgatgaaa gtttatcagt tgccggaagc cactcgtggc gcgcgcggtc gtccgatcgt
     5761 caacctgctg ccgctggagc aggacgaacg tatcactgcg atcctgccag tgaccgagtt
     5821 tgaagaaggc gtgaaagtct tcatggcgac cgctaacggt accgtgaaga aaactgtcct
     5881 caccgagttc aaccgtctgc gtaccgccgg taaagtggcg atcaaactgg ttgacggcga
     5941 tgagctgatc ggcgttgacc tgaccagcgg cgaagacgaa gtaatgctgt tctccgctga
     6001 aggtaaagtg gtgcgcttta aagagtcttc tgtccgtgcg atgggctgca acaccaccgg
     6061 tgttcgcggt attcgcttag gtgaaggcga taaagtcgtc tctctgatcg tgcctcgtgg
     6121 cgatggcgca atcctcaccg caacacaaaa cggttacggt aaacgtaccg cagtggcgga
     6181 atacccaacc aagtcgcgtg cgacgaaagg ggttatctcc atcaaggtta ccgaacgtaa
     6241 cggtttagtt gttggcgcgg tacaggtaga tgactgcgac cagatcatga tgatcaccga
     6301 tgccggtacg ctggtacgta ctcgcgtttc agaaatcagc atcgtgggcc gtaacaccca
     6361 gggcgtgatc ctcatccgta ctgcggaaga tgaaaacgta gtgggtctgc aacgtgttgc
     6421 tgaaccggtt gacgaggaag atctcgatac catcgacggc agtgccgcgg aaggggacga
     6481 tgaaatcgct ccggaagtgg acgttgacga cgagccagaa gaagaataat tttacttctt
     6541 catgccaaaa gggagctatc tcccttgttt gaattgaaaa gtccaggctg caaagtctgg
     6601 gcttttgtcg tattagggca cggtaaagtt tggctgtgcc cgtaaaaaat ggctggctat
     6661 acacaaggaa tgtggcaatg agtggtgaaa aaaaggcgaa aggctggcgg ttctatggtc
     6721 ttgtaggttt tggcgcaata gtactgcttt ccgctggcgt ctgggcgttg caatatgctg
     6781 gcagtgggcc agaaaaaacg
//
LOCUS       DAJTOR010000198         6790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTOR010000198 DAJTOR010000000
VERSION     DAJTOR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.198"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..83)
                     /locus_tag="OO249_003681"
     CDS             complement(<1..83)
                     /locus_tag="OO249_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HCR5637817.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVG"
     gene            complement(77..1315)
                     /gene="preT"
                     /locus_tag="OO249_003682"
     CDS             complement(77..1315)
                     /gene="preT"
                     /locus_tag="OO249_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5637818.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(1712..1951)
                     /locus_tag="OO249_003683"
     CDS             complement(1712..1951)
                     /locus_tag="OO249_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5637819.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(1954..2673)
                     /gene="sanA"
                     /locus_tag="OO249_003684"
     CDS             complement(1954..2673)
                     /gene="sanA"
                     /locus_tag="OO249_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5637820.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(2823..3707)
                     /gene="cdd"
                     /locus_tag="OO249_003685"
     CDS             complement(2823..3707)
                     /gene="cdd"
                     /locus_tag="OO249_003685"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5637821.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(3837..4532)
                     /locus_tag="OO249_003686"
     CDS             complement(3837..4532)
                     /locus_tag="OO249_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5637822.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYGSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(4529..4927)
                     /locus_tag="OO249_003687"
     CDS             complement(4529..4927)
                     /locus_tag="OO249_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5637823.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            5166..6116
                     /gene="dusC"
                     /locus_tag="OO249_003688"
     CDS             5166..6116
                     /gene="dusC"
                     /locus_tag="OO249_003688"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5637824.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            complement(6503..6586)
                     /gene="yohP"
                     /locus_tag="OO249_003689"
     CDS             complement(6503..6586)
                     /gene="yohP"
                     /locus_tag="OO249_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5637825.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         1770 a         1731 c         1684 g         1605 t
ORIGIN      
        1 ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa acttcacgcc gcaaaaagta
       61 atcgaaagat ctttcgttaa catgagcaag ctccctctaa atagtgatga atcgcctcgg
      121 cggcttcttt cccggttttc acggcataga ccatggtttt gtcaccctca acaatatcgc
      181 cagcagcaaa gacttgcggg tcgcgggtct ggtaattttg tgttttgatg gtgttacgct
      241 gcggctctaa ctccgcaaag gcatctagtc tggcatgctg accgacggcg agaataattt
      301 tatctgccgc catcgtcagt tcgcccgata accgtacatg cttaaacgtg actttattgc
      361 cttccacggc tactggcgtg aatccatcga tgatcgaaac gcccagctcc cgggcgctgg
      421 taaactcttt ttcgcttgcc ggaaactcat ctaactcttc acgcgctacg caagttaccg
      481 cctgacagcc gagaactttc agcgtgctgg ctacgtccat cgtgacatca ccgccgccga
      541 taattaatgc gctttgtgga atgctgatat cgccttgcgc ctgtcgtgca cgttgcaaga
      601 agtcgacggc aatctcaacg tcactatgct caaacagcgg tagcccggaa ccgcttgata
      661 accccacggt gaccagtacc gcgcggtttt ctgctttcag ctgctcaagg gtgagtgtgt
      721 taccgacttc gttgttgcac ttaatggtca cgcccatttt ttcaatacgg gcgatctctg
      781 catccagtac tgattgcggt aaccggaatt gcggaatgcc gttacgcaac cagccaccgg
      841 gttgtgcttc tttctcatag atcgtgacgt cataaccctg gtttgtcagt gtcacactgg
      901 cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt gccgagcgtt ttagtaccgg
      961 gctgataaat ttccatcccg gtttgttgtt caaaatcagt aacaaaacgc tgtaagcggc
     1021 cgatatcaat gggagcatca acaccggcac gggtacaacc gctttggcat aatttctccg
     1081 tcgggcacac tctggcacaa acggcaccga gggcattatt ttcgcgaatt gtctcggcag
     1141 cgcctttaaa attacgaaag tagattgagc gaataaattt ccccggatcg gtctgtgccg
     1201 ggcaagcctg actacaggga gcgtcgtgac ataataaaca gcgagaggct tctttaatcg
     1261 ccagtaacgg cgtaaaagcc ggagtgagtt catccagata attttgttgc ggcatgtgat
     1321 cttccttaat gctatgagta attaaggcga acgctaaata tactcgtcat acttcaagtt
     1381 gcatgtgctg cgtctgcggg ttattcggcc catccatggg cctcacccct tatggggccg
     1441 ctgcaggcag cgttcaaatc cgctccatgc ggatttgtcg ctcaccccag tcacttactt
     1501 atgtaagctc ctggggactc actcacttgt cgccttcctg caactcgaat tatttagagt
     1561 ataaatattg cgtgctgata ttaaggctat gcgcgtgggg aaatttatac tgtgaaagga
     1621 ttcacaaata gctgcggttc agaatgttat atccgcagct tattgagtga agtaataatt
     1681 attctcaata attaacagaa aagagaaaat attaacgata gaaaataaga taaacaatca
     1741 tgccgataga taaaattccg aagccgatat atgccaccat ataagcgaaa aagagtccgg
     1801 gattttttgc tcgccagaca tacaccggtt ccggtgcatt tttaacccgt ttatcaatcg
     1861 cgcctgcgcg ccagcctttc catgcttcgc ggaaacaaaa tatcggaata aggaaaaaaa
     1921 cggcaacgac aaaaaactgc tgtacatcca taactacttt ccttgtttct tttgtaattc
     1981 aagtaactgt tcgggtgtga cggcgggata cccctgcgca tcttccggca cctggtgcat
     2041 agccggaata gggaccagcg gccctaaaaa acgcggttca cgtttaaaaa tataaaggtc
     2101 agccagcgca ccgaaacggg cggcaaattc gcgaatacgt actgacagca tatctttcgg
     2161 tgacggtacg gcataacact gagcctgaat ccccatatgc agcgcaataa ataatgcccg
     2221 ctcacagtgg aaacgttggg tgataataat gaaatcatta gtatcgaaaa ctttgcgtgt
     2281 acgcacgatg gaatccagcg tacgaaagcc tgcgtaatcg agaacaatat ctgatgggtc
     2341 gacaccagca gcgattaaat ctttgcgcat ggtcatcggc tcattataac tttgcaatgc
     2401 gttatcgccg ctcagtaata gataatttac cttaccgctg ttataggcat taatcgctcc
     2461 ttgaatgcgg tagcgataat actgattaat tacgccagta cgataatatt ttgctgttcc
     2521 gagcaccaca ccgacctggc ggtaggggag atcctgcaat tcgtcgtaga tataaggcgc
     2581 ggttttccag ctcatccagc gatcgaggcc gagcacagtc aacaacagca agccgatcag
     2641 gactaacagg ctgaggaaca cgcgctttaa catgcaggag actcgatcta aggaaaattt
     2701 ccatcaggct actttacccg ctggttttgc gcaagaaacg gcaaactgtt tgccgggaat
     2761 ttcgacacca ggagagggct ggcaagccgg acaaggcgtt cacgccgcat ccggcaccag
     2821 gcttaagcga gaagcactcg gtcgatactg tgacagccga gagctttcag cgttgcggag
     2881 gtggcatccc actgaatcaa cggcgcatcg gctttttctg ccagaaccgc gcgctggata
     2941 tccgggtaat cataaccctt gagattcaac agaattaacg ctccctgcaa cggtggcaga
     3001 gtcgggttga atgcggcgtt ttcagcgtag ctgccactga aaatacgacc gtctttacat
     3061 tccagcgcga caccgcttgg cgacttactg taaggcatgt gcgaacggtt tgccgctgca
     3121 atcgccgcct gagaaagcgc atcaccagtc agcgcatagc cgtgatcctg ctcgtccatc
     3181 agcagcgttt taatctccag atctttcggc ccaaaggcat ccggcagata gtcacgcagc
     3241 gcgtgtgcct cgcggcccgg cagatgaata cgcagatcca gaccgctgtt cagttcattc
     3301 ataaactgac ggcagtgacc acaaggcgtg tagttaacgg tgatggctgc aagcgctttt
     3361 tcaccactca accaggcgtg gctgatcgcg ctttgttcgg catgaacggt ttgctgcatt
     3421 gtcgcaccaa taaattccat attggcaccg aaataccagg ttccgctcac accgcgcgca
     3481 attgcgccaa cattaaaatt cgacaatggc gtacgcgcac aggccgctgc cagcggaagt
     3541 agtgcgaatg ccagcgcgtc ttcgtccagc cccgttgcgc tcttcagcga tgagacttgc
     3601 tccccggtca acaaagcggg gaagtacttg tctgccagaa taggttccag tgcagattgc
     3661 aagttatccg caagttgggc aaaagcggtt tgaaaacgtg gatgcatggc gttgcctcat
     3721 aatcatgtaa tgggataaca gtttacgaac ccgttatggc tttatatgtg atctgaatct
     3781 cattaattat gcaacgcgtg aaacttacat caaaaatgcg cgacgcatcg caaattttag
     3841 cccattactg ccagaataat cgggaaaagg aacggtgcga tcagcgaagt aattatcccg
     3901 cataacacca gcgctagcga actaaatgca ccttcctgat aatccagctc ggcgcagcgc
     3961 gccgtaccga gggcgtgcga ggcagtcccc attgccagac cgcgcgcagc tttggtacga
     4021 atacgcatcg cattaagcaa tgtatggcca aataccgcgc cgaggatgcc gacgaaaatc
     4081 acgcaaactg cgctgattgc cggaataccg ccaatactgc cgccaaccgc cattgcaata
     4141 ggcgtggtga ctgatttcgg caggattgac gcggcgattt ccggtgaagc gcccatcaac
     4201 aatgccacgg aagtccccgt caccatcgca accacgctgc cgataaaaca gatggtgatg
     4261 atcgatttcc agcgcgcgcg gatctggtgt agctgctcat ataaaggata ggccagcgcg
     4321 acgaccgccg gttgcagcag atcgtttagc acttcgctac ctttaaagta gctgccgtaa
     4381 gagatgccgg tcagcattaa aaaagggatg atcaccacca ttgctaccag caacgggtta
     4441 agcaagggaa acttataccg tgccgccagt ttgcgggcgg caaaaaagac gatcaatgtc
     4501 agtggtaatg accaccagat attagccatc attcttctga tcctttctga cctacgactt
     4561 tacgttcacc gtgcaccagt tgcgaactcc agctcaccac cagaaaaacc accagtgtac
     4621 tgacagcaca ggaaaccact accgggccaa actgtgcgcg gagcaaatca aaatattgca
     4681 tgacgcctac gccaatcggc acaaacaata gcgccatata gcgaatcagt acgtagcacc
     4741 ccggattgac ccattttgcc ggaagaattt gcaaggccag caggacaaac aggatcagca
     4801 tcccgatgat gctgcccgga atggttaccg gtagcaggga agcaatgaaa atgcctgcat
     4861 acaggcaggc ataaatcagg acgaaagcgc gtaaatattg ccagataatg ttcagtgtct
     4921 tactcataat caacacccca gatgagatga attcatcata caattaattc gtaaaacgtg
     4981 ctaccgatca cattaagaaa tcagcgctta ttttttcgct ccccggcatt acgcaatgcg
     5041 caacgaactc aggcatacat caagcataga gagggattac agtttgcagc aatccctctt
     5101 tgcagacctt taatccgttg accataggcg ctaacatagc gcctcatttt tttgcgggtg
     5161 aagatatgcg tgtgttactg gctccgatgg agggtgtgct tgactctctg gtgcgtgaat
     5221 tgctgaccga agttaacgac tacgatctgt gcatcaccga gtttgtccgc gtggtggatc
     5281 aactgctgcc ggtaaaagtt tttcatcgca tttgccctga gctacaaaac gccagccgga
     5341 caccatccgg tacgctggtg cgcgtgcagt tgttgggtca gttcccacaa tggctggcag
     5401 agaatgccgc ccgtgcggtg gaattaggtt cctggggcgt ggatctcaat tgcggctgcc
     5461 cgtcgaaaac ggtcaacggt agcggtggtg gggcgacgtt actcaaagat cctgaactca
     5521 tctaccaggg tgcaaaagcg atgcgtgaag ctgtaccggc gcatttgccc gtcagcgtga
     5581 aagtgcgtct gggctgggac agcggtgaga agaaatttga aatcgccgat gcggttcaac
     5641 aggctggtgc tacggagctg gtggtgcatg gacggacaaa agagcagggt taccgcgcgg
     5701 agcatattga ctggcaggcg attggcgaga ttcgccagcg gctgaatatt ccggtgattg
     5761 ccaacggtga aatctgggac tggcagagtg cgcaacaatg tatggcgatc agcggctgcg
     5821 acgcagtgat gattggtcgc ggggcgctca atattcccaa cctgagccgg gtggtaaaat
     5881 ataacgaacc gcgaatgccg tggccggagg tggttgcttt gctgcaaaaa tatacccgtc
     5941 tggaaaagca gggcgatacc gggttatatc acgttgcgcg gattaaacag tggttgagtt
     6001 atttgcgtaa agaatacgat gaagcaacgg aattatttca gcatgttcgg gtgttgaata
     6061 attcccctga tattgcaagg gctattcagg caattgatat cgataagctg cgataatagt
     6121 ggaatcgcag tttatcagag acaatgaatc gcttattcag cttcatcatc ttgttgcgct
     6181 cctttcatga gctaagccac atattgccac tggcgcaagg agcgcgcagg gggaggacaa
     6241 tcgcctcctc cccctgcccc ctgggctctg gcgaacaaaa tcgccgctgc gcggtacctt
     6301 cagcttatcc ccttcggcta ccgggtcggg cgcgaggtaa cgtccttgta agacgcgccc
     6361 tcagcccaca tccatgtggg ctgccccggc cttcggggaa cgcttcagcg attttgacgc
     6421 caccaacacc ggagctgttt gcaaatcaag tacacaaaat atctagttcc tgacctgaca
     6481 acttatatgc catatctgat ttttaaaata tcatcttaaa tacgccagtc accaccagta
     6541 gcccaatcag gaaaataatc aatacagccc agagtataat tttcattggc gttcctttat
     6601 ttgtgagtgt tgaataagta taggaaaaaa cgaagccctt tgccgttatc cataaacgat
     6661 taagataaat cggaagatat cattcactta gtctatattt ttctgggtat gtcgttttat
     6721 tgctactgat gtattgccac gcgaaactaa aaaatatatc gcaacacacg gtaatctccg
     6781 cgcaccgttt
//
LOCUS       DAJTOR010000199         6732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTOR010000199 DAJTOR010000000
VERSION     DAJTOR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.199"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..649
                     /locus_tag="OO249_003690"
     CDS             <1..649
                     /locus_tag="OO249_003690"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5637826.1"
                     /translation="TYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
                     QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGH
                     AAFVDAGKLLPNIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAE
                     LLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            740..1987
                     /gene="tnaB"
                     /locus_tag="OO249_003691"
     CDS             740..1987
                     /gene="tnaB"
                     /locus_tag="OO249_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5637827.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2119..3294
                     /gene="mdtL"
                     /locus_tag="OO249_003692"
     CDS             2119..3294
                     /gene="mdtL"
                     /locus_tag="OO249_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5637828.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3269..4228
                     /gene="yidZ"
                     /locus_tag="OO249_003693"
     CDS             3269..4228
                     /gene="yidZ"
                     /locus_tag="OO249_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5637829.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            4385..5134
                     /locus_tag="OO249_003694"
     CDS             4385..5134
                     /locus_tag="OO249_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5637830.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5156..5722
                     /gene="yieF"
                     /locus_tag="OO249_003695"
                     /gene_synonym="chrR"
     CDS             5156..5722
                     /gene="yieF"
                     /locus_tag="OO249_003695"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5637831.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5776..>6732)
                     /gene="adeP"
                     /locus_tag="OO249_003696"
     CDS             complement(5776..>6732)
                     /gene="adeP"
                     /locus_tag="OO249_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5637832.1"
                     /translation="TIFRVRYWMIANIPVSLRVGITSGIGLFIGMMGLKNAGVIVANS
                     ETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLGWMLGDVHYNGI
                     VSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVTDKAGLADEKGK
                     FPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAVVVGLLFLLVIF
                     LSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAVMMPFSFSITEG
                     IALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1584 a         1712 c         1707 g         1729 t
ORIGIN      
        1 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat
       61 gggcggcttg ctgtgcatga aagacgacag cttctttgat gtgtacaccg agtgcagaac
      121 cctttgcgtg gtgcaggaag gcttcccgac atatggcggc ctggaaggcg gcgcgatgga
      181 gcgtctggcg gtaggtctgt atgacggcat gaatctcgac tggctggctt atcgtatcgc
      241 gcaggtacag tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg
      301 cggtcacgcg gcattcgttg atgccggtaa actgctgccg aatatcccgg cagaccagtt
      361 cccggcacag gcgctggcgt gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat
      421 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga
      481 actgctgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
      541 tgaagccttt aaacatgtga aagagaacgc ggcgaatatt aaaggattaa cctttaccta
      601 cgaaccaaaa gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca
      661 gagtggctat aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc
      721 ttcctctaaa ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg
      781 tgttatggtt atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct
      841 tgccggtgcc tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct
      901 tcattccggg ttattgttat tagaagcaaa tttaaattat cctgtcggct ccagttttaa
      961 caccatcacc aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc
     1021 cttcgttctc tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga
     1081 aacgatatca atgaatttgg gctatcacgc taatccacgt attgtcggga tctgcacagc
     1141 cattttcgtt gccagcgtat tgtggataag ttcgttagcc gccagtcgca ttacctcatt
     1201 gttcctcggg ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt
     1261 cgattactcc atcctgcgcg atgccaccag caccactgcg ggaacgtctt acttcccgta
     1321 tatctttatg gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag
     1381 cctgattatt tgctatggaa aacgcaaaga taagttaatc aaaagcgtgg tatttggttc
     1441 gctgctggcg ctggtgattt atctcttctg gctctattgc acgatgggga atattccgcg
     1501 cgaaagcttt aaggcgataa tctcctcagg cggcaacgtt gattcgctgg tgaaatcgtt
     1561 cctcggcacc aaacagcacg gcattatcga gttttgcctg ctggtgttct ctaacttagc
     1621 tgttgccagt tcgttctttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt
     1681 taagattgat aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc
     1741 tgcgttgttg tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccgggct
     1801 gtgcgccacc atctgggcgg tcattattcc cgcagtgctt gcaatcaaag ctcgcaagaa
     1861 gtttcccaat cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct
     1921 ctttggtata accgtgattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt
     1981 tggctaaatc cttcaagaag ccagccattc gctggcttct tgcctctcag gaaatcactt
     2041 atgtccaaat ggcaactcgc ctgatcctcc ttcaccacgt atgctttgcg tcaccttact
     2101 atcaggacgc tttagcccat gtcccgcttt ttgatttgta gttttgccct ggttttactt
     2161 tatcccgccg ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat
     2221 gccagcgaag cgcagttgca tattgcgttc tccgtatatc tggcggggat ggcagctgcg
     2281 atgttatttg ccggtaaagt ggccgatcgt tcagggagaa agccggtcgc catacccggc
     2341 gcggcgctat ttattattgc ctcggtgttc tgttcactgg ctgaaaccag cacgttattt
     2401 cttgcaggcc gatttctaca ggggttgggc gcaggctgtt gttacgtagt ggcgttcgcc
     2461 attttgcgcg acacgctgga tgatcgacgt cgggctaaag tgctgtcatt actcaacggt
     2521 attacctgca tcattccggt gttagcgcca gtgctcggac atctgattat gcttaaattc
     2581 ccgtggcaga gtctgttctg ggcgatggca atgatgggca tcgcggtact gatgttgtct
     2641 ttgtttattt taaaagaaac gcgcccagcg tcccccgctg cttcggacaa accacgagaa
     2701 aatagcgagt cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc
     2761 gtttcggtga tcctcacttt cgtcaataca tcgccggtat tgctgatgga aatcatgggt
     2821 tttgagcgcg gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt
     2881 tcattctcca cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctcg
     2941 caggtgttat tccttgcagc ggggatcacc cttgccgttt caccttccca tgcggtttct
     3001 ctgtttggta tcacactgat ttgcgccggt ttctcggtag gttttggcgt agcgatgagt
     3061 caggcgttag gaccattttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg
     3121 caggtttgcg gttcgtcact gtggatttgg ctggcagcgg tggttggtat cggcgcatgg
     3181 aatatgctga tcgggattct gattgcctgt agcatagtga gcctgttgct gattatgttc
     3241 gtcgcgcctg gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa
     3301 tctgctgctc tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg
     3361 gatgaacgtg accccttcag cggtgagtaa gtcgctggca aagttaagag cgtggtttga
     3421 cgacccgctc tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat
     3481 ggagcaaaat ctggcggaat ggatgcaaat gagcaatctg ctgctggata aaccgcacca
     3541 ccagacaccg cgcggcctga agtttgagtt ggcggcggaa tcgccgctga tgatgatcat
     3601 gcttaatgcg ctgtcgaaac ggatctacca acgttacccg caggcgacca tcaaactacg
     3661 taactgggat tacgattcct tagatgccat tactcgtggt gaagtggata ttggtttttc
     3721 cggtcgcgaa agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga
     3781 ttatgaagtg ctgtttagtg atgtaccctg cgtctggtta cgcaaagatc atccggcact
     3841 gcatgaaacg tggaatctgg acaccttttt acgttatccg catatcagca tttgctggga
     3901 acagagcgac acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat
     3961 tgctatgagc ctgccggaat ttgagcagtc actgtttatg gctgcgcaac ccgacaatct
     4021 gctactggcg accgcgccgc gctactgtca gtactacaat caactccatc aactgccgtt
     4081 ggttgctctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag ttccttttac
     4141 cctgctgtgg cataaacgga acagtcataa tccgaagatc gtctggttac gggaaaccat
     4201 taaaaacctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc caatttcaca
     4261 ataaaatgta aaaaagttgt aataagcttg tctgaatcga acttttagcc gctttagtct
     4321 gtccatcatt ccagtaaatg attactctta tattcataat ggaccattaa gcatggagcg
     4381 aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg
     4441 tctcccctcc cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt
     4501 tctggcgtcc agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt
     4561 gccggaaatc gtcaccctgc cgaaaggtaa accggttttc agtgataaaa atttgccttc
     4621 gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg
     4681 tggcctcgat atggaactac agcgtgcgac gcgcgggttt catagcccac acgcgcccga
     4741 taaccacacc ttttccagca atgaatcgct atggatcagt aaacaaaacg atcctaacga
     4801 agcgcgggcg cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt
     4861 gaatgacgat ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca
     4921 tgtaaatcat gtagaagcgt tatgcgacgc ggaggacgtg ctggtgtggt ccgtggcggt
     4981 cacgcccgcg attgaaaagc tcagtgtctg ggagttagat ggcaaacaca gctggaaaag
     5041 cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca
     5101 gctctctacc gtgaagtctt tttcgccaaa ttgatagaca accacaggag tcattatgtc
     5161 tgaaaaattg caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat
     5221 ggttgcacgt accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc
     5281 cattgccgac attcccctgt atgacgctga cgtacagcag gaagaaggtt ttccagcaac
     5341 ggttgaagct ctggcggaac agatccgtca ggctgacggt gtggtgatcg tcacgccgga
     5401 atataactac tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc
     5461 ggatcaaccg ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg
     5521 cggcgcgcgc tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat
     5581 gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatcggc aaaccggaga
     5641 agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt
     5701 tattcagcga gttaagatct aaataaaaaa cccgccagta atcatgcatg gcgggttttt
     5761 aatgcgctat cgattttaat gagcgtcgat aaatacaatc ttcaggataa acagcagcgc
     5821 aacgatgatt acgcacgggc taaggtcacg cagacgcccg gtaccaatct tcatcacgca
     5881 gtaggagata aagcccagcg caataccttc ggtaatcgaa aagctgaacg gcatcatcac
     5941 ggcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact
     6001 tgaggtcatc aacacgccaa cgtagatcaa cgcgccagct gcagcgtagc ctggcaccat
     6061 tcccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt
     6121 cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga
     6181 agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt
     6241 catgcgcggg aatttcccct tctcatccgc cagacctgct ttatcggtca cgccaatcag
     6301 cgtaccggag gagtcaaaca agttgaccaa catgaaagag aaaatcaccc ctgccagccc
     6361 gaggttaaac gacccggcta aatctacatg acccacaact gtcattacgc tcggcggcgc
     6421 agaaacgatg ccattgtagt gcacatcacc cagcatccag cccagcagcg tcgtcaccac
     6481 gatagaaacc agcaccgctg cgtgaatgtt gcgcgaggcc agaatagcaa tgatgaagaa
     6541 gccgaggata cccagaagta cgctgtgaga agtcagatta ccgatgctca ccagcgtttc
     6601 cgagttagcg acaatcacac ctgcgttttt cagccccatc atgccgataa acaggccgat
     6661 accgctggta atacccacac gcagacttac cggaatgtta gcaatcatcc agtaacgaac
     6721 gcggaaaatg gt
//
LOCUS       DAJTOR010000200         6695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTOR010000200 DAJTOR010000000
VERSION     DAJTOR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.200"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            80..1996
                     /gene="dnaK"
                     /locus_tag="OO249_003697"
     CDS             80..1996
                     /gene="dnaK"
                     /locus_tag="OO249_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5637833.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2085..3215
                     /gene="dnaJ"
                     /locus_tag="OO249_003698"
     CDS             2085..3215
                     /gene="dnaJ"
                     /locus_tag="OO249_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5637834.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGQHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3319..3471)
                     /gene="mokC"
                     /locus_tag="OO249_003699"
     CDS             complement(3319..3471)
                     /gene="mokC"
                     /locus_tag="OO249_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5637835.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            4057..5223
                     /gene="nhaA"
                     /locus_tag="OO249_003700"
     CDS             4057..5223
                     /gene="nhaA"
                     /locus_tag="OO249_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5637836.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5289..6188
                     /gene="nhaR"
                     /locus_tag="OO249_003701"
     CDS             5289..6188
                     /gene="nhaR"
                     /locus_tag="OO249_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5637837.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(6226..>6695)
                     /locus_tag="OO249_003702"
     CDS             complement(6226..>6695)
                     /locus_tag="OO249_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5637838.1"
                     /translation="RFNYNLNTILLVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHI
                     RFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDG
                     TNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT         1705 a         1577 c         1735 g         1678 t
ORIGIN      
        1 ggcagttgaa accagacatt tcgcccctat tacagactca caaccacatg atgaccgaat
       61 atatagtgga gacgtttaga tgggtaaaat aattggtatc gacctgggta ctaccaactc
      121 ttgtgtagcg attatggatg gcaccactcc tcgcgtgctg gagaacgccg aaggcgatcg
      181 caccacgcct tctattattg cctataccca ggatggtgaa actctggttg gtcagccggc
      241 taaacgtcag gcagtgacga acccgcaaaa caccctgttt gcgattaaac gcctgattgg
      301 ccgccgcttc caggacgaag aagtacagcg tgatgtttcc atcatgccgt tcaaaattat
      361 tgctgctgat aacggcgacg catgggtcga agttaaaggc cagaaaatgg caccgccgca
      421 gatttctgct gaagtgctga aaaaaatgaa gaaaaccgct gaagattact tgggtgaacc
      481 ggtaactgaa gctgttatca ccgtaccggc atactttaac gatgctcagc gtcaggcaac
      541 caaagacgca ggccgtatcg ctggtctgga agtaaaacgt atcatcaacg aaccgaccgc
      601 agctgcgctg gcttacggtc tggacaaagg tactggcaac cgtactatcg cggtttatga
      661 cctggggggt ggtactttcg atatttctat tatcgaaatc gacgaagttg atggcgaaaa
      721 aaccttcgaa gttctggcta ccaacggtga tacccacctg ggtggtgaag acttcgacag
      781 ccgtctgatc aactacctgg ttgaagaatt caagaaagat cagggcattg acctgcgcaa
      841 cgatccgctg gcaatgcagc gcctgaaaga agcggcagaa aaagcgaaaa tcgaactgtc
      901 ttccgctcag cagaccgacg ttaacctgcc gtacatcact gcagatgcga ccggtccgaa
      961 acacatgaac atcaaagtga ctcgtgcgaa actggaaagc ttggttgaag atctggtaaa
     1021 ccgttccatt gagccgctga aagttgcact gcaggacgct ggcctgtccg tatctgatat
     1081 cgacgacgtt atcctcgttg gtggtcagac tcgtatgcca atggttcaga agaaagttgc
     1141 tgagttcttt ggtaaagagc cgcgtaaaga cgttaacccg gacgaagctg tagcaatcgg
     1201 tgctgctgtt cagggtggtg ttctgactgg tgacgtgaaa gacgtactgc tgctggacgt
     1261 taccccgctg tctctgggta tcgaaaccat gggcggtgtg atgacgacgc tgatcgcgaa
     1321 aaacaccact atcccgacca agcacagcca ggtgttctct accgctgaag acaaccagtc
     1381 tgcggtaacc atccatgtgc tgcagggtga acgtaaacgt gcggctgata acaaatctct
     1441 gggtcagttc aacctggatg gtatcaaccc ggcaccgcgc ggcatgccgc agatcgaagt
     1501 taccttcgat atcgatgctg acggtatcct gcacgtttcc gcgaaagata aaaacagcgg
     1561 taaagagcag aagatcacca tcaaggcttc ttctggtctg aacgaagatg aaatccagaa
     1621 aatggtacgc gacgcagaag ctaacgccga agctgaccgt aagtttgaag agctggtaca
     1681 gactcgcaac cagggcgacc atctgctgca cagcacccgt aagcaggttg aagaagcagg
     1741 cgacaaactg ccggctgacg acaaaactgc tatcgagtct gcactgactg cactggaaac
     1801 tgctctgaaa ggtgaagaca aagccgctat cgaagcgaaa atgcaggagc tggcacaggt
     1861 ttcccagaaa ctgatggaaa tcgcccagca gcaacatgcc cagcagcaga ctgccggtgc
     1921 tgatgcttct gcaaacaacg cgaaagatga cgatgttgtc gacgctgaat ttgaagaagt
     1981 caaagacaaa aaataatcgc cctataaacg ggtaattata ctgacacggg cgaaggggaa
     2041 tttcctctcc gcccgtgcat tcatctaggg gcaatttaaa aaagatggct aagcaagatt
     2101 attacgagat tttaggcgtt tccaaaacag cggaagagcg tgaaatcaaa aaggcctaca
     2161 aacgcctggc catgaaatac cacccggacc gtaaccaggg tgacaaagag gccgaggcga
     2221 aatttaaaga gatcaaggaa gcttatgaag ttctgaccga ctcgcaaaaa cgtgcggcat
     2281 acgatcagta tggtcatgct gcgtttgagc aaggcggcat gggcggcggc ggttttggcg
     2341 gcggcgcaga cttcagcgat atttttggtg acgttttcgg cgatattttt ggcggcggac
     2401 gtggtcgtca acgtgcggcg cgcggtgctg atttacgcta taacatggag ctcaccctcg
     2461 aagaagctgt acgtggcgtg accaaagaga tccgcattcc gactctggaa gagtgtgacg
     2521 tttgccacgg tagcggtgca aaaccaggta cacagccgca gacctgtccg acctgtcatg
     2581 gttctggtca ggtgcagatg cgtcagggtt tctttgccgt gcagcagacc tgtccacact
     2641 gtcagggccg cggtacgctg atcaaagatc cgtgcaacaa atgtcatggt catggtcgtg
     2701 ttgagcgcag caaaacgctg tccgttaaaa tcccggcagg ggtggacact ggagaccgca
     2761 tccgtcttgc gggcgaaggt gaagcgggcc agcatggcgc accggcaggc gatctgtacg
     2821 ttcaggttca ggttaaacag cacccgattt tcgagcgtga aggcaacaac ctgtattgcg
     2881 aagtcccgat caacttcgct atggcggcgc tgggtggcga aatcgaagta ccgacccttg
     2941 atggtcgcgt caaactgaaa gtgcctggcg aaacccagac cggtaagctt ttccgtatgc
     3001 gcggtaaagg cgtcaagtct gtccgcggtg gcgcacaggg tgatttgctg tgccgcgttg
     3061 tcgtcgaaac accggtaggc ctgaacgaga agcagaaaca gctgctgcaa gagctgcaag
     3121 aaagcttcgg tggcccaacc ggcgagcaca acagcccgcg ctcaaagagc ttctttgatg
     3181 gtgtgaagaa gttttttgac gacctgaccc gctaacctcc ccaaaagcct gcccgtgggc
     3241 aggcctgggt aaaaataggg tgcgttgaag atatgcgagc acctgtaaag tggcggggat
     3301 cactccccgc cgttgctctt actcggattc gtaagccgtg aaaacagcaa cctccgtctg
     3361 gccagttcgg atgtgaacct cacagaggtc ttttctcgtt accagcgccg ccactacggc
     3421 ggtgatacag atgacgatca gggcgacaat catcgcctta tgctgcttca ttgctctctt
     3481 ctccttgacc ttacggtcag taagaggcac tctacatgtg ttcagcatat agggggcctc
     3541 gggttgatgg taaaatatca ctcggggctt ttctctatct gccgttcagc taatgcctga
     3601 gacagacagc ctcaagcacc cgccgctatt atatcgctct ctttaaccca ttttgtttta
     3661 tcgattctaa tcctgaagac gcctcgcatt tttgtggcgt aattttttaa tgatttaatt
     3721 atttaacttt aatttatctc tttatcgcaa ttattgacga caagctggat tatttttgaa
     3781 atattgtcct aacaaataac gccgactgac aagaaattaa ttattacttt tcctaattaa
     3841 tccctcagaa atcctcacct taagctatga ttatctgagc ttagggtcac tcgtcagcgc
     3901 ttacagccgt caaaaactca tctcaccgct gatggcgaaa attctacaaa ggctcgtaaa
     3961 aaacgaatta ttcctacact ataatctgat tttaacgatg attcgtgcgg ggtaaaatag
     4021 taaaaacgat ttattcacct gaaagagaaa taaaaagtga aacatctgca tcgattcttt
     4081 agcagtgatg cctcgggagg cattattctt atcattgccg ctatcctggc gatgatgatg
     4141 gccaacagcg gcgcaaccag tggatggtat cacgactttc tggagacgcc ggttcagctc
     4201 cgggttggtt cactcgaaat caacaaaaac atgctgttat ggataaatga cgcgctgatg
     4261 gcggtatttt tcctgttagt cggtctggaa gttaaacgtg aactgatgca aggatcgcta
     4321 gccagcttac gccaggccgc atttccagtt atcgccgcta ttggtgggat gattgtgccg
     4381 gcattactct atctggcctt taactatgcc gatccgatta cccgcgaagg gtgggcgatc
     4441 ccggcggcta ctgacattgc ctttgcactt ggagtgctgg cgctgttggg aagtcgtgtt
     4501 ccgttagcac tgaagatctt tttgatggct ctggctatta tcgacgatct tggggccatc
     4561 attatcatcg cattgttcta cactaatgac ttatcgatgg cctctcttgg cgtcgcggct
     4621 gtagcaattg cggtactcgc ggtattgaat ctgtgtggtg tacgccgcac gggcgtctat
     4681 attctggttg gcgtagtgtt gtggactgcg gtgttgaaat cgggggttca cgcaaccttg
     4741 gcgggggtaa ttgtcggctt ctttattcct ttgaaagaga agcatgggcg ttctccagcg
     4801 aagcgactgg agcatgtgtt acacccgtgg gtggcgtatc tgattttgcc gctgtttgca
     4861 tttgctaatg ctggcgtttc actgcaaggc gtcacgctgg atggcttgac ctccattctg
     4921 ccattgggga tcatcgctgg cttgctgatt ggcaaaccgc tggggattag tctgttctgc
     4981 tggttggcgc tgcgtttgaa actggcgcat ctgcctgagg gaacgactta tcagcaaatt
     5041 atggcggtgg ggatcctgtg cggtatcggt tttactatgt ctatctttat tgccagcctg
     5101 gcctttggta gcgtagatcc agaactgatt aactgggcga aactcggtat cctggtcggt
     5161 tctatctctt cggcggtaat tggatacagc tggttacgcg ttcgtttgcg tccatcagtg
     5221 tgacaggacg gtttaccggg gagccataaa cggctccctt ttcattgtta tcagggagag
     5281 aaatgagcat gtctcatatc aattacaacc acttgtatta cttctggcat gtctataaag
     5341 aaggttccgt ggttggcgca gcggaggcgc tttatttaac tccacaaacc attaccggac
     5401 agatccgggc tctggaagag cgcctgcaag gcaaattatt taagcgcaag ggacgtggtc
     5461 tcgaacccag cgagctggga gaactggtct atcgctatgc cgataaaatg ttcaccttaa
     5521 gccaggaaat gctggatatt gtgaactatc gcaaagaatc caatttattg tttgacgttg
     5581 gcgtggctga tgcactttcc aaacgcctgg tcagtagcgt gctgaatgct gcagtggtag
     5641 aaggcgagcc gattcatctt cgctgcttcg aatccaccca cgaaatgcta ctggagcaat
     5701 taagccagca taaactggat atgatcattt ccgactgtcc gatagactct acgcagcagg
     5761 aaggcctgtt ctccgtgaga attggcgaat gtggcgtgag tttctggtgt acaaatccac
     5821 caccagaaaa accgttcccg gcttgtctgg aagaacggcg acttttgatt cctgggcgac
     5881 gttcaatgtt agggcgcaaa ttgcttaact ggtttaactc ccagggatta aacgtagaaa
     5941 tcctcggcga gtttgatgat gccgcgttga tgaaagcttt tggtgcaatg cacaatgcaa
     6001 tcttcgttgc cccaacgctt tatgcatatg acttttatgc cgataaaact gtcgtagaaa
     6061 ttggtcgcgt cgagaatgtg atggaagagt accatgctat ttttgctgag cggatgattc
     6121 agcacccggc ggtacagcga atctgcaata cggattattc tgcgcttttt agtccagcgg
     6181 cgcgttaatc ggcagctccc ccaaagttaa agtggaggag atagattagt tgtacataac
     6241 cacgattttg actcggctca ttatttgccc gcttgagaca ttatttccat atgtacgcgg
     6301 gcgaataaat agaggaatct gattacttcc ttcatgggga tgctgaaaag agtagtaatt
     6361 gcttttacca tactggattt cattcgtacc atcattggag agttgaaagc ctacttgacc
     6421 cgcaccgtcc tcactgtttt ctaactcatt ttttaatatg ctggttcctg actcctgtat
     6481 cgtcactggt tcaaagtaat attcaacatc actgggttca aaaactaatt caccttgctc
     6541 gccctggatg cagtcaaatc ttatatgatg aaactgataa ttttcattat tgatacgaga
     6601 cagtacaaat cgtccagtgt cgatggattg atcttcaaaa ccacaggtca ttaaatttac
     6661 cagtaaaata gtatttaaat tataattaaa gcgga
//
LOCUS       DAJTOR010000201         6564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTOR010000201 DAJTOR010000000
VERSION     DAJTOR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.201"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..357
                     /locus_tag="OO249_003703"
     CDS             <1..357
                     /locus_tag="OO249_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637839.1"
                     /translation="AITALNFYQGDVPSVMIRIQPENMSPLIIDISTGEHDNYIIQTL
                     DVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTAPTHFNKTCITSDC
                     WQILFFSPDHFNNDFY"
     gene            379..1557
                     /locus_tag="OO249_003704"
                     /pseudo
     CDS             379..1557
                     /locus_tag="OO249_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
     gene            complement(1945..2321)
                     /gene="rnpB"
                     /locus_tag="OO249_003705"
     ncRNA           complement(1945..2321)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO249_003705"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2354..3499)
                     /gene="garK"
                     /locus_tag="OO249_003706"
     CDS             complement(2354..3499)
                     /gene="garK"
                     /locus_tag="OO249_003706"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5637840.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTVL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(3596..4486)
                     /gene="garR"
                     /locus_tag="OO249_003707"
     CDS             complement(3596..4486)
                     /gene="garR"
                     /locus_tag="OO249_003707"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5637841.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4516..5286)
                     /gene="garL"
                     /locus_tag="OO249_003708"
     CDS             complement(4516..5286)
                     /gene="garL"
                     /locus_tag="OO249_003708"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR5637842.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(5302..6538)
                     /gene="garP"
                     /locus_tag="OO249_003709"
                     /pseudo
     CDS             complement(5302..6538)
                     /gene="garP"
                     /locus_tag="OO249_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
BASE COUNT         1794 a         1705 c         1432 g         1633 t
ORIGIN      
        1 gcaattactg cattgaattt ctatcaaggc gatgttcctt ctgtcatgat ccgaatccaa
       61 ccggaaaata tgagtccatt aatcatagat atttctacag gagaacatga taattatatc
      121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
      181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
      241 acccaaaaag aagccatctt aacagcccct acgcacttta ataaaacctg cattacttca
      301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
      361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
      421 ttagtccgtc gaaaaatgaa tgaccttcct gaaatcccca tagaaaataa tctcgaagca
      481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
      541 gcgttacaac caggtggaaa atgtattatc actggtgatt ttcaaaaaga attagcgtac
      601 ttacaaaatg taattcttta taatgactcg tttcttcgtc tggatttttt aggttataac
      661 gcccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
      721 aaccaggaaa tatctacagg caatatcaat gttaattgtc cattaaaaga tatttacaat
      781 gaaatcagga ggttgaacac aacctttagt tgtgggactg gagatatagt tgatctatcc
      841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
      901 aatactattc taaatccttt taaattggat tcaacaaatt ttactaatgc caacatgttt
      961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
     1021 ataacgcctg ttttaataag tattagcgat atgtattctg aagaaaaaat caagtttgtc
     1081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
     1141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
     1201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
     1261 gagatgaaag aattctcatc aaaaaaaaac atatgattac atcgatataa tcattgaaga
     1321 ttatgaaaat atcaaagaaa atgcttatct ggtcgtcccc caaattaatt atactatgga
     1381 tttaaacata gaagactcca gctcagaaga gttaataccc tcgagaaaga cgaaaattct
     1441 tcggacaatg gctttgaagt cgaagaatat aacgcatatg aagcatataa ctcagagaat
     1501 ctatatttta ccagagagga atatacgtat gattacgacc tttcaaatgc aatatagact
     1561 taataacttc ttacaaaatc tataattaat accgtagata ttcattcatc ttaccctgtg
     1621 caccaaaagc aaaaactcac tgacgcgggt ttgaaactga tggcaaccgc aaaaatgcct
     1681 gatgcgctac gcttatcagg cctacgccat ctctgcaata tattgaattt gcgtgctttt
     1741 gtaggcagga taaggcgttc acgccgcatc cggcatgaat aaagcgcact caacaatctg
     1801 aaacccgccg gaacggttta ttacgtacat caggtgaaac tgaccgataa gccgctttct
     1861 tttgggtata gtgtcgtgga cagtcattca tctttctgcc cctccaaaag caaaaacccg
     1921 ccgaagcggg tttttacgta aatcaggtga aactgaccga taagccgggt tctgtcgtgg
     1981 acagtcattc atctaggcca gcaatcgctc actggctcaa gcagcctacc cgggttcagt
     2041 acgggccgta ccttatgaac ccctatttgg ccttgctccg ggtggagttt accgtgccac
     2101 ggactgttac cagccgcgcg gtgcgctctt accgcaccct ttcaccctta cctgatcccg
     2161 cttgcgcggg ccatcggcgg tttgctctct gttgcactgg tcgtgggttt cccccccagg
     2221 cgttacctgg caccctgccc tatggagccc ggactttcct cccctccgcc cgtctccccc
     2281 gaagaggacg acgacgaagc ggcgactgtc tggtcagctt cggcgcgcag tatagagggt
     2341 ttgcgcgccc ttgtcacccc gcgttgcgca ttccaatcgc cagtgtcgcg gcgatattac
     2401 gtgaagcacg gcagatattg tcataagccc cgcggaatgc ttcgtccaac gtacctatgc
     2461 tggtcaatac gctgaagacc gcatcaatgc catgctgatg tacaacgcca acatcatcgg
     2521 tcaggctacc cgcaatgcca atcaccggtt tatggtactt cttcgccacg tttgcgacac
     2581 caatcggtac cttgccgtga atactctggc tgtcaatacg cccttcaccg gtgatcacca
     2641 gcgtacaatc gtgaatatgt tcttccagat tcagcaccgt agtgacgatt tcaataccac
     2701 ttttcagttc cgcaccaaga aacgccatca gcgccgcgcc cataccgccc gcagctcctg
     2761 cgccggggac atctttcaca tcaacatgca gcgctttttt aatgacctcg gcatagtgag
     2821 agaggttatt gtccagctca acaatcatcg cttcactggc tcccttttgt gggccaaaga
     2881 tgcgcgatgc gccgttatcg cccaccagcg gattggtgac atcacaagcg acgcgaatga
     2941 cgcaatcttt taagcgcgga tcgaggccgg aaatatcaat atcattcaga gtattaagac
     3001 taccgccgcc aaaaccaatt tcattgccgt tggcgtcgca taatttcgcc cccagcgcct
     3061 gtaccatgcc tgcgccgcca tcatttgtag cgctgccgcc aatgccgata ataatgtttg
     3121 tcgcaccgct ctccagcgcc tgcaggatta actcgcctgt gccgcgtgaa gtggtcacga
     3181 gtggatcgcg tttttccgca ggtaccagct ccagcccact ggccgccgcc atttcaataa
     3241 acgcggtttt gccatcgccg gagatccccc aactggcatt cactttctcg cccaacggcc
     3301 ctgtaaccca ggcgtgacgt tcggaaccct gggtggctgc aatcatcgct tccaccgttc
     3361 cttcgccacc gtcggcaacc ggaacagaaa cgtactgtgc atcaggaaaa atttcccgaa
     3421 atcctttttc tatcgcctgc gcaacctcgc tggcagataa actttcttta taagagtctg
     3481 gggcgattac gattttcata cctatgcctg ttaccacatg acgccggagg gcgtttctct
     3541 tattcggcgt ggattccagg cccggattgc aatacgccat ccgggcacga cgtcattaac
     3601 gagtaacttc gactttcgcc agtttttcgt agtagcacgc cagagcgcta tgatccgccg
     3661 ttcctaaacc atctgcacgc agtgcctgca tcatctccat aaccgcagct gtgagcggca
     3721 gttgcgcgcc gacgccgtga gaagtatcca gcgcattcgc cagatcctta atatgcagat
     3781 caatgcggaa gcccggcttg aagttgcggt ccatcaccat cggcgctttg gcatccagca
     3841 cggtactgcc cgccagccca ccgcgaattg cctgataaac caggtccggg ttaacgcccg
     3901 ctttagttgc cagcgttaac gcttctgaca tcgcagcaat attcagcgcc acaatgacct
     3961 gatttgccag tttggtgacg ttacctgcac cgatttcccc ggtatgcacc acggaacccg
     4021 ccatcgcttt catcaaatca tagtatttgt cgaaaatagc cttgtcgccg cccaccatca
     4081 ctgacagcgt accgtcgatg gctttcggtt caccgccgct caccggagca tccagcatat
     4141 caatgccttt cgctttcagc gcttcgctga tttcacggct tgccagcggt gcgatagaac
     4201 tcatatcgat caataccgta cctggcttcg cgccttcaat aatgccattc tcacccagcg
     4261 ccacctcttt cacatgaggg gagtttggca gcatggttat gatgacgtcg cactgttcag
     4321 cgatcgcttt agccgtagac gctgtttctg cacctgcagc aatcacgtca gcaatagctt
     4381 ctgggttacg gtcagcaacc accagcgagt aacctgcttt cagaaggttt ttactcattg
     4441 gtttacccat aatccccagg ccaataaaac caactttcat agtcatatca atcatctctc
     4501 ttgttgcggt ggtggttatt ttttaaaggt atcagccagt ttctgagtgg cagagcggaa
     4561 gacgccaaga tcgctgccga cagccacaaa cgtcgcgccc cattccagat aacgacgcgc
     4621 atcggcttcg accggcgcga gaataccgct gggtttgccg tgcgcactgg cacggttaaa
     4681 aatatgctga attgcttttt gtacatccgg atgcgaggca ttgccgagat ggcccaatgc
     4741 cgcagccaga tcgctggggc cgacgaagat accgtctaca ccttcggtag cggcaatagc
     4801 atcgacgtta tctacgccct gctgactttc tatctggacc agaatagtga tgttcttgtt
     4861 cgactgagcg aaataatccg ccacggtgcc aaacatattg gcgcggtgag aaacggagac
     4921 gccgcgaatg ccttccggcg ggtaacgagt tgatgccacc gcctgctctg cttcctcttt
     4981 tgtttctaca aaaggaatca ggaagttata gaaaccgata tccagaagac gcttaataat
     5041 taccggctcg ttggtcggca ctcgcactac tggcgcgctg gcgctgcctt tcagggccat
     5101 taactgcgga ataaacgtgg aaatatcgtt tggcgcatgt tcgccatcca gcaccagcca
     5161 gtcaaaccca gccaaaccaa gaacttcagt gctaatcggg ttagagagtg ctgaccagca
     5221 accaatttgt acctgtttcg cagccagtgc ggctttgaat ttattcggga aaacatcgtt
     5281 attcatcgct tatacctttg cttatttctg caattccata cgtttaatgt cgccaactac
     5341 gaagaggtag cagaccatcg ccatcagcgc tgaacatccc acgaaaacca gtgctgcatt
     5401 gaaggagtgc agttcactta ccaggtagcc aatcaccagt ggagtgacaa tggaggcaac
     5461 attgccaaag acgttaaaga cgccgccaca gaggccaaca atctctttcg gcgcggtgtc
     5521 agaaatcacc ggccagccca gagcaccaaa tcctttgcca aaaaaagcca gcgccatcag
     5581 catgaccacc agcgtggtgt tgttggtgta gttacataag atgatggtgg aagccagcaa
     5641 cattcccagc acaatcggta gcttacgtgc cagggtcagg gataaaccgc gtttgatcag
     5701 ataatccgag aagacacctc ccagcacgcc gcccgcaaaa ccacacagtg ccggaatcga
     5761 ggcgaccaga cccactttca gaatcgacat gcctttttcc tgcaccagat aaatcgggaa
     5821 ccaggtgagg aagaaccagg tgatggtgtt gataaaatat tgtccgaaaa atacgcccaa
     5881 catcatgcgg ttagagagca attgcttgat gtaatgcagt ttgggaccgc ttgctgccgc
     5941 gctgcccggc tttttgtggt ccatatcgac caccgcgcca ttttcagaga taaacttcag
     6001 ctcttccgca gacatacgtg ggtgatctgt cgggttataa atcaacttga tccacagcgc
     6061 cgtcagcaca aaaccaatca cccccataac ggtgaagacg tgctcccagc cccaggcgaa
     6121 agtcagccag ccaagcagag gcgaaaagag cgccagcgag aaatattgcg ccgagttaaa
     6181 gatggcggag gcagtaccac gttctttcgt cgggaaccag gcggcgacaa tacgggcgtt
     6241 cgccgggaat gatggcgctt ccgagaagcc gagcataaag cgcataaaga acatagagat
     6301 ccctgcccag gccagcggga acatatcaac aaagccttgc aggaaggtga atagcgacca
     6361 gaaaaagagg ctgtaggtgt aaactttttt cgagccaaac ttatcaagca gccagccgcc
     6421 gggaatttgc atcagcaggt aggacttcgg taccagcaat agacagcgtt gcacaatcgg
     6481 cgtagttaac ggcggtaaca ataaaaataa tcagtaatat taaatagcgg gtatgcacgc
     6541 ctttcttttt tacgtcaacg gtgt
//
LOCUS       DAJTOR010000202         6508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTOR010000202 DAJTOR010000000
VERSION     DAJTOR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.202"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(623..2332)
                     /locus_tag="OO249_003710"
     CDS             complement(623..2332)
                     /locus_tag="OO249_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5637843.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="OO249_003711"
     CDS             complement(2764..3483)
                     /locus_tag="OO249_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5637844.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3608..4171)
                     /locus_tag="OO249_003712"
                     /pseudo
     CDS             complement(3608..4171)
                     /locus_tag="OO249_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4155..4413
                     /locus_tag="OO249_003713"
                     /pseudo
     CDS             4155..4413
                     /locus_tag="OO249_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4522..5100)
                     /locus_tag="OO249_003714"
     CDS             complement(4522..5100)
                     /locus_tag="OO249_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637845.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTCIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKDLYKCV"
     gene            complement(5521..5758)
                     /locus_tag="OO249_003715"
                     /pseudo
     CDS             complement(5521..5758)
                     /locus_tag="OO249_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5945..6374)
                     /locus_tag="OO249_003716"
                     /pseudo
     CDS             complement(5945..6374)
                     /locus_tag="OO249_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1786 a         1490 c         1188 g         2044 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcaccct ctggatgtga cctttatcca
      421 tgaagaagat ttggccatac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtag
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaacatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt gactcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgaga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaatat cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgagtagct
     2641 cagcagttta tgagccgaat gctctgtcac ggtcaggaat caggctgcat acgctgtgcc
     2701 aaatctaatc gtagttattc taaccagttt tccagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tactctgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagcgtaag ggtcttctcc gatcatggtg
     3601 ggaaggctta gaacgccata ttcagtttcc catagtggaa catcactccc agcttaaagc
     3661 gctcccggtt gcggtagccc ttggctttta ttctcagcag cctgatcttg ctgttcagtg
     3721 cttccgcatt tccgtttgat acgccatggc gcatggcgtt caggataccg tacaaccttt
     3781 tccctatcgt tttagccaca ttcttcatca tggggacgtc actgttggcc gccagcgaca
     3841 gccatctctg ccagtcgttg cgtctttcct cgctccatgg tctgctccag atatcctttg
     3901 ccagttcttt cagcgcccag cacaggctcg tcagcttcat ctgcgcacgc agccatatca
     3961 gcttttcctg cctggattcg gtcatccatt tatcgctgtg ctgccacagg aagcgagttc
     4021 cctttgcctg acgacgactt tcaacaggaa gatgcggatg ttcattctgg cgggttttat
     4081 ccaccacctc acccggttgt ttcgccacat gaaagcgatc gaaggctatt ttctcaaccg
     4141 cattgggtaa gtggatgcgc gctgctctta gtttgctgag ccagtttagc attgccttct
     4201 acatgaccga tgcctgagcc ggtataccga actttattgt cctcaaccag tcatgttata
     4261 agcaagaaat gcactcagcg gatagagcga cataatctga acctgcggac acatcttaag
     4321 cggcttaccc gcaaaaccat atgcttctca aagtctgatg atatgcacta taagatcatc
     4381 ggctggtatc tgacaatcaa ccaccaccac taaatctgcg tcacgacctg caactgagtc
     4441 tggttgaaca ggctgaggcc ctgctgaagc aacgcgaacc tctcgactct tgttggattt
     4501 taccaaatag tttttctaaa attaaacaca tttatataag tcctttgctg aacaaaacgc
     4561 agcccgccat cctttttcat tcataaattc agcaatggca gataaaactt tttcatcaag
     4621 catataaaaa ataatgcaag tagactcaat atgcctcata caaataaggc cgcacatatc
     4681 cgtaatatat ttgtcaatgc tgctataatt tgcatggtaa tatgccatgc tattatctga
     4741 tgattttaca tatgcagtat gacatatttt tccttcacga ttaacaaaac tgacaaaacc
     4801 actttcacca ggagtaatat ataaagatga atttaacgtg cgctcactaa tattcattaa
     4861 ttgttgaaat tcaatggaat agttttcagt taatgtatct attatcttgg ctttgtcttt
     4921 ctttgttatc tcattgtctt tttcggcaag aaaaattgcc actttatgac atgcattcag
     4981 ctcgttaatt ttacctgata aaactgcttt tcttaccaca tcgttgaact catcgatagt
     5041 gtgtttttta ttatatatat catgaaaaat atagcctaaa gacttaaacg agactttcat
     5101 tttatattct ccttaaatat taacaggata tatatatcac attttcctct atattttcag
     5161 aaaccagcta tcattttccc cattactggt acgtcgcttc agatatttcc gcagcaagcg
     5221 ataaccctgc gcactatgag gatgccattc attataatgt ttgaacacct tcgcgagact
     5281 actttactgc tgttaacacg tcaggttcga acataacact gatttaatca tatttcatcg
     5341 ttttgacgaa gttttctgcc attccatttt gcaccgccgt gtgtttgggg gcaaggccga
     5401 ccatctgtgt ttcattcgcc agatataagt gatgagatgg cattctccct ccttcacggc
     5461 agaagcattt tgaccgttta ggggatgcgg acgattttca tgcctgctaa gatactttcg
     5521 ttactgatgc tgtccgtttt ccaccatttc atcatcactg attggcgttg tgtttttggt
     5581 cagcactcca gctttacgtt tctcttttaa ccggtaactc tctcctttga tattcagcgt
     5641 gtagcagccg atctaggatc gctgttgcca gcacgtgatc tccgaacatt tctccccagt
     5701 ctgcgacccc tttgtttgac gtcagtatga tgctcgcttt ttcatatcga cagttcatcg
     5761 ataggaattt aaatccccaa aagattaaaa aagcaccgaa gaatgcgcgt ttattcaaca
     5821 caacctgatc atcaagtaat cggtatagac acttaatatt gcttacattg attgattcaa
     5881 tgtatctctg atggtttttt gctccgttcc cctcaacatg gacgcaactt cccctctgcg
     5941 actctcagcc gcaccaccgt attcgagcca gcggctcatg catcataacc tgctctgcca
     6001 gacggtaacc cctcttcagc cccgtaaacc gcatctgacc tccgcacagc tcgcacttca
     6061 gcgggtcaac cttcagtcac tgctgataca tccctctcct tgtgatttgc ctcgccgttt
     6121 ttctcactgt ctccgttatg gcgtacacca catcttccag taatcgccgc ttcaccggac
     6181 tcaggacccc gtatcaccat ccggaacccc ttatccgcca catgccagga gaacctttcc
     6241 ataaactcgt cgccactcat cacaaggttt tcttctcgtt ttgtccggtg gctgttgtaa
     6301 cgcaggccaa tttcatcctg accggcataa tgttccaggc ggctcatctg caccggctgc
     6361 ttctccagcc agttaagcca tcggtttccc tgctgaaaaa tatcggcaaa acggggaagc
     6421 atcagaaagg cggaataacg ccatccggcc agtgaaccgt accgcattca gggcaataca
     6481 tgccgtcggc gttgataccg gcaggaat
//
LOCUS       DAJTOR010000203         6436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTOR010000203 DAJTOR010000000
VERSION     DAJTOR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.203"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            299..1669
                     /gene="glmU"
                     /locus_tag="OO249_003717"
     CDS             299..1669
                     /gene="glmU"
                     /locus_tag="OO249_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5637846.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQESNTGILISNGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            1831..3660
                     /gene="glmS"
                     /locus_tag="OO249_003718"
     CDS             1831..3660
                     /gene="glmS"
                     /locus_tag="OO249_003718"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5637847.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            3820..4641
                     /locus_tag="OO249_003719"
     CDS             3820..4641
                     /locus_tag="OO249_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5637848.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            4628..>6436
                     /locus_tag="OO249_003720"
     CDS             4628..>6436
                     /locus_tag="OO249_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR5637849.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDE"
BASE COUNT         1702 a         1414 c         1671 g         1649 t
ORIGIN      
        1 acgcgtgatc cgtcctgaag agcgttcaca tagatcctgc tgatataaaa cacccctgtt
       61 ttcccgttta ttcattgatc gaaataagag caaaaacatc cacctgacgc ttaaatgaag
      121 gtactgcctt aattttctgc agacaaaagg cgtgacgatg gtcgaaaatg gcactttcgt
      181 cagcggggat aatccgttat tgaacaattt atcctctgtc catttcacga tgaaaaaaat
      241 gtagtttttt caaggtgaag cggtttaaat tcgttctcaa attacagtca ggacgcgtat
      301 gttgaataat gctatgagcg tagtgatcct tgccgcaggc aaaggcacgc gcatgtattc
      361 cgatcttccg aaagtgctgc atacccttgc cgggaaagcg atggttcagc atgtcattga
      421 tgctgcgaat gaattaggcg cagcgcacgt tcacctggtg tacggtcacg gcggcgatct
      481 gcttaaacag gcgctgaaag acgacaacct taactgggtg cttcaggcag agcagctggg
      541 tacgggtcat gcaatgcagc aggccgcacc tttctttgcc gatgatgaag acattttaat
      601 gctctacggc gacgtgccgc tgatctctgt cgaaacactc cagcgcctgc gtgatgctaa
      661 accgcagggt ggcattggtc tgctgacggt gaaactggat gatccgaccg gttatggacg
      721 tatcacccgt gaaaatggca aagttaccgg cattgttgag cacaaagatg ccaccgacga
      781 gcagcgtcag attcaggaga gcaacaccgg cattctgatc tctaacggcg cagatatgaa
      841 acgctggctg gcgaagctga ccaacaataa tgctcagggc gaatactaca tcaccgacat
      901 tattgcgctg gcgtatcagg aagggcgtga aatcgtcgcc gttcatccgc aacgtttaag
      961 cgaagtagaa ggtgtgaata accgcctgca actctcccgt ctggagcgcg tttaccagtc
     1021 cgaacaggct gaaaaactgc tgttagcagg cgttatgctg cgcgatccgg cgcgttttga
     1081 tctgcgcggt acgcttactc acgggcgcga tgttgaaatt gatactaacg ttatcatcga
     1141 gggcaacgtg actctcggtc atcgcgtgaa aatcggcacc ggttgcgtga ttaaaaacag
     1201 cgtgattggc gatgattgcg aaatcagtcc gtataccgtt gtggaagacg tgaatctggc
     1261 agcggcctgt actattggcc cgtttgcccg tctgcgtcct ggtgctgagt tgctggaagg
     1321 tgcacacgtc ggtaactttg ttgagatgaa aaaagcgcgt ctgggtaaag gctcgaaagc
     1381 tggtcatctg acttacctgg gcgatgcgga aattggcgat aacgttaaca tcggcgcggg
     1441 aaccattacc tgcaactacg atggtgcgaa taaatttaag accattatcg gcgacgatgt
     1501 gtttgtcggt tccgacactc agctggtggc cccggtaaca gtaggcaaag gcgcgaccat
     1561 tgctgcgggt acaactgtga cgcgtaatgt cggcgaaaat gcattagcta tcagccgtgt
     1621 gccgcagact cagaaagaag gctggcgtcg tccggtaaag aaaaagtaat tctggccggg
     1681 taatccggtc atctgggatg aggagataac ataatctccc tcccacaagc agtaactata
     1741 aaaataaccc cactctctac aaggctcggg gcgcccgaaa aaacgggcat acaggttgac
     1801 cgacaacgat ataaatcgga atcaaaaact atgtgtggaa ttgttggcgc gatcgcgcaa
     1861 cgtgatgtag cagaaatcct tcttgaaggt ttacgtcgtc tggaataccg cggatatgac
     1921 tctgctggtc tggccgttgt tgatgcggaa ggtcatatga cccgcctgcg tcgcctcggt
     1981 aaagtccaga tgctggctca ggcagcggaa gaacatcctc tgcatggcgg caccggtatt
     2041 gctcacactc gctgggcgac ccacggtgaa ccttcagaag ctaacgcgca tccgcatgtt
     2101 tctgaacaca ttatggtggt gcataacggc atcatcgaaa accatgaacc gctgcgtgaa
     2161 gagttaaaag cgcgtggcta taccttcgtt tctgaaaccg acaccgaagt gattgcccat
     2221 ctggtgaact gggagctaaa acaaggcggg actctgcgtg aggctgttct gcgtgctatc
     2281 ccgcagctgc gcggtgcgta cggtacagtg atcatggact cccgtcaccc ggataccctg
     2341 ctggcggcac gttctggtag tccgctggtg attggcctgg gtatgggcga aaactttatc
     2401 gcttctgacc agctggcgct gctgccggtg acccgtcgct ttatcttcct tgaagagggc
     2461 gatattgcgg aaattactcg ccgttcggta aacatcttcg ataaaactgg cgcggaagta
     2521 aaacgtcagg atatcgaatc caatctgcaa tatgacgcgg gcgataaagg tatttaccgt
     2581 cactacatgc agaaagagat ctacgaacag ccgaacgcga tcaaaaacac ccttactggg
     2641 cgcatcagcc acggtcaggt tgatttaagc gagctgggac cgaacgccga cgaactgctg
     2701 tcgaaggttg agcatattca gatcctcgcc tgtggtactt cttataactc cggtatggtt
     2761 tcccgctact ggtttgaatc gctggcaggt attccgtgcg acgtcgaaat cgcctctgaa
     2821 ttccgctatc gtaaatctgc cgtgcgtcgt aacagcctga tgatcacctt gtcacagtct
     2881 ggcgaaaccg cggataccct ggcgggcctg cgtctgtcga aagagctggg ttaccttggt
     2941 tcgctggcaa tctgtaacgt tccgggttct tctctggtgc gcgaatccga tctggcgctg
     3001 atgaccaacg cgggtacgga aatcggcgtg gcgtccacta aagcattcac cactcagtta
     3061 actgtgctgt tgatgctggt ggcgaagctg tctcgcctga aaggtctgga tgcctccatt
     3121 gaacatgaca ttgtgcatgg tctgcaggcg ctgccgagcc gtattgagca gatgctatct
     3181 caggacaaac gcattgaagc tctggcagaa gatttctctg acaaacatca cgcactgttc
     3241 ctgggccgtg gcgatcagta cccaatcgca ctggaaggcg cattgaagct gaaagagatc
     3301 tcctacattc acgctgaagc ctacgctgct ggcgagctga aacacggccc gctggcgctg
     3361 attgacgcgg atatgccggt tatcgtcgtt gcaccgaaca acgaactgtt ggaaaaactg
     3421 aaatccaaca ttgaagaagt tcgcgcgcgt ggcggtcagt tgtatgtctt cgccgatcag
     3481 gatgcgggtt ttgtaagtag cgataacatg cacatcatcg agatgccgca tgtggaagag
     3541 gtgattgcac cgatcttcta caccgttccg ctgcagctgc tggcttacca tgtcgcgctg
     3601 atcaaaggca ccgacgttga ccagccgcgt aacttggcaa aatcggttac ggttgagtaa
     3661 tcactatcat agataaaaaa gggcttgtgg gcggacaata aagtcttaaa ctgaacaaaa
     3721 tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca
     3781 gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca
     3841 ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa
     3901 gactatattc catggctaac agtacaagaa gttccttctt caggtcgttc ccaccgtatt
     3961 tattctcata agacgggacg agtccatcat ttgctatctg acttagagct tgctgttttt
     4021 ctcagtcttg agtgggagag cagcgtgcta gatatacgcg agcagttccc cttattacct
     4081 agtgatacca ggcagattgc aatagatagt ggtattaagc atcctgttat tcgtggtgta
     4141 gatcaggtta tgtctactga ttttttagtg gactgcaaag atggtccttt tgagcagttt
     4201 gctattcaag tcaaacctgc agcagcctta caagacgagc gtaccttaga aaaactagaa
     4261 ctagagcgtc gctattggca gcaaaagcaa attccttggt tcatttttac tgataaagaa
     4321 ataaatcccg tagtaaaaga aaatattgaa tggctttatt cagtgaaaac agaagaagtt
     4381 tctgcggagc ttttagcaca actatcccca ttggcccata tcctgcaaga aaaaggagat
     4441 gaaaacatta tcaatgtctg taagcaggtt gatattgctt atgatttgga gttaggcaaa
     4501 acattgagtg agatacgagc cttaaccgca aatggtttta ttaagttcaa tatttataag
     4561 tctttcaggg caaataagtg tgcagatctc tgtattagcc aagtagtgaa tatggaggag
     4621 ttgcgctatg tggcaaatta atgaggttgt gctatttgat aatgatccgt atcgcatttt
     4681 ggctatagag gatggccaag ttgtctggat gcaaataagc gctgataaag gagttccaca
     4741 agctagggct gagttgttgc taatgcagta tttagatgaa ggccgcttag ttagaactga
     4801 tgacccttat gtacatcttg atttagaaga gccgtctgta gattctgtca gcttccagaa
     4861 gcgcgaggag gattatcgaa aaattcttcc tattattaat agtaaggatc gtttcgaccc
     4921 taaagtcaga agcgaactcg ttgagcatgt ggtccaagaa cataaggtta ctaaggctac
     4981 agtttataag ttgttacgcc gttactggca gcgtggtcaa acgcctaatg cattaattcc
     5041 tgactacaaa aacagcggtg caccagggga aagacgttca gcgacaggaa cagcaaagat
     5101 tggccgagcc agagaatatg gtaagggtga aggaaccaag gtaacgcccg agattgaacg
     5161 cctttttagg ttgaccatag aaaagcacct gttaaatcaa aaaggtacaa agaccaccgt
     5221 tgcctataga cgatttgtgg acttgtttgc tcagtatttt cctcgcattc cccaagagga
     5281 ttacccaaca ctacgtcagt ttcgttattt ttatgatcga gaatacccta aagctcagcg
     5341 cttaaagtct agagttaaag caggggtata taaaaaagac gtacgaccct taagtagtac
     5401 agccacttct caggcgttag gccctgggag tcgttatgag attgatgcca cgattgctga
     5461 tatttattta gtggatcatc atgatcgcca aaaaatcata ggaagaccaa cgctttacat
     5521 tgtgattgat gtgtttagtc ggatgatcac gggcttttat atcggctttg aaaatccgtc
     5581 ttatgtggtg gcgatgcagg cttttgtaaa tgcttgctct gacaaaacgg ccatttgtgc
     5641 ccagcatgat attgagatta gtagctcaga ctggccgtgt gtaggtttgc cagatgtgtt
     5701 gctagcggac cgtggcgaat taatgagtca tcaggtcgaa gccttagttt ctagttttaa
     5761 tgtgcgagtg gaaagtgctc cacctagacg tggcgatgct aaaggcatag tggaaagcac
     5821 ttttagaaca ctacaagccg agtttaagtc ctttgcacct ggcattgtag agggcagtcg
     5881 gatcaaaagc catggtgaaa cagactatag gttagatgca tctctgtcgg tatttgagtt
     5941 cacacaaatt attttgcgta cgatcttatt cagaaataac catctggtga tggataaata
     6001 cgatcgagat gctgattttc ctacagattt accgtctatt cctgtccagc tatggcaatg
     6061 gggtatgcag catcgtacag gtagtttaag ggctgtggag caagagcagt tgcgagtagc
     6121 gttactgcct cgccgaaagg tctctatttc ttcatttggc gttaatttgt ggggtttgta
     6181 ttactcgggg tcagagattc tgcgtgaggg ttggttgcag cggagcactg atatagctag
     6241 acctcaacat ttagaagcgg cttatgaccc agtgctggtt gatacgattt atttgtttcc
     6301 gcaagttggc agccgtgtat tttggcgctg taatctgacg gaacgtagtc ggcagtttaa
     6361 aggtctctca ttttgggagg tttgggatat acaagcacaa gaaaaacaca ataaagccaa
     6421 tgcgaagcag gatgag
//
LOCUS       DAJTOR010000204         6393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTOR010000204 DAJTOR010000000
VERSION     DAJTOR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.204"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..208)
                     /locus_tag="OO249_003721"
     CDS             complement(<1..208)
                     /locus_tag="OO249_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR5637850.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDN"
     gene            687..2465
                     /locus_tag="OO249_003722"
     CDS             687..2465
                     /locus_tag="OO249_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCR5637851.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(2505..2984)
                     /gene="xdhC"
                     /locus_tag="OO249_003723"
     CDS             complement(2505..2984)
                     /gene="xdhC"
                     /locus_tag="OO249_003723"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5637852.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2981..3859)
                     /gene="xdhB"
                     /locus_tag="OO249_003724"
     CDS             complement(2981..3859)
                     /gene="xdhB"
                     /locus_tag="OO249_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5637853.1"
                     /translation="MFDFASYHRAATLADAINLLANNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAAMSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3870..6167)
                     /gene="xdhA"
                     /locus_tag="OO249_003725"
     CDS             complement(3870..6167)
                     /gene="xdhA"
                     /locus_tag="OO249_003725"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5637854.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAIAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGTFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
BASE COUNT         1595 a         1511 c         1616 g         1671 t
ORIGIN      
        1 agttgtcgcg gaatacggaa atacctaatc cactattaaa gactttggtt gagatgtttt
       61 cagcacgcag tgcttttaat gctgcggcaa cgtccagtac ttgtttcagt tcatctggcg
      121 tctgttccca cgttaacaaa aaatctttct cgtgaaggtg agaggtcagc gaattgatat
      181 ccttaatcag ctcattaaca gttttcatca taaagtcccg gtagtggaat gacaggcaaa
      241 gtggtttatt tcactgtgac ttgccaaagt gattggtgtt ttcacagcta tacctgggac
      301 taacaataag tgtgccagaa ttatccgcgc ggctgcaaaa gactaatttg acggataatg
      361 tgtggggttg atcacaaatt gatgccagct tctggttgtt tgtaaaatac gaaaacgttt
      421 ggcttttttt atcgcgttat ttatttgctg ctaagtatca gaaataagat aaaggggcag
      481 gctatctctc ttcagtttga taaactcacc gccgtaattc tcatattgat atagaaatgt
      541 gatatctctt ccataatttc attagaaaat gataaaaatc ttatgttttt atcaaagcca
      601 atttcgcctg agttaatatc atcatgatct gtgaaccatc aacgtcttca atgatcgact
      661 tttaagtaag tctggaaggt aaagctatgg agcttgctac tacgcagtca gtattgatgc
      721 aaattcaacc gacaattcag cgttttgcca gaatgcttgc cagcgttttg cagcttgagg
      781 ttgagatcgt tgatgaaaac ttgtgtcgcg tcgccggaac gggcgcgtat gggaagtttc
      841 ttggtcgcca gttgagcggc aactcacgcc tgctccgcca cgtcctggaa acgaaaactg
      901 aaaaagttgt gacacagtct cgcttcgatc ccctttgcga aggttgcgat agtaaagaaa
      961 attgccgtga aaaagcattt ctgggtacgc ctgtcatttt acaggatcgt tgtgttgggg
     1021 tgataagttt gattgccgtt acccacgagc aacaagagca tatcagtgat aatttacgcg
     1081 aattttctga ttatgttcgc catatatcca ccatttttgt ttcgaaactt ctggaggatc
     1141 aggggccagg agataacatc agtaaaatat tcgcgaccat gatcgataat atggatcagg
     1201 gcgtattagt tgttgatgat gaaagtcggg ttcagtttgt taatcagact gccttaaaaa
     1261 cacttggtgt tgtacaaaat aatattattg ggaaacctat ccgtttcaga ccattaacat
     1321 ttgagagtaa ttttactcat ggacatatgc agcatattgt ttcgtgggac gataaaagtg
     1381 aattaatcat tggtcaattg cataacattc agggccgaca attattttta atggcatttc
     1441 accaatcgca taccagtttt tctgtagcaa atgcacctga tgaaccacat attgaacaat
     1501 tggttggcga gtgccgtgtt atgcggcaat taaaacgact cattagccgt attgcaccca
     1561 gcccatccag cgttatggtg gttggtgaaa gcggcacggg taaagaagtc gtcgcccgag
     1621 caatccataa gttgagcgga agacggaata aaccctttat tgctatcaac tgtgccgcga
     1681 ttccggagca gcttctggaa agcgaactgt tcggttatgt taaaggcgca tttactggcg
     1741 cttctgccaa cggtaaaaca gggttgattc aggcggcgaa tacgggcacg ctgtttctcg
     1801 atgaaatagg tgatatgcca ttaatgttgc aggctaaatt actgcgcgct attgaggcgc
     1861 gtgaaattct gccgattggt gccagtagcc caatacaagt cgacattcgc atcatttctg
     1921 caactaatca gaatttggcc cagttcattg ccgaaggtaa attccgcgaa gatctcttct
     1981 accgacttaa tgttatcccg ataactctgc caccgctgcg tgaacgtcag gaagatattg
     2041 aactattggt gcattacttt ttacatctgc atacccgtcg tctgggatcg gtttatcctg
     2101 gcattgctcc cgatgtcgtc gaaatattgc gtaagcatcg ttggcccgga aacctgcgcg
     2161 agttaagcaa tttgatggaa tatctggtta acgtagttcc ttcaggtgaa gttatcgaca
     2221 gcacgctatt gccgccaaat ctgctgaata atggcacaac ggagcaaagt gatgtaacag
     2281 aggtcagtga ggcgcacctg tcactcgatg atgcgggcgg cacggcgctg gaggagatgg
     2341 aaaagcaaat gatccgcgag gcgctttcac gtcataacag caagaagcaa gttgctgatg
     2401 aactgggcat cggcattgct acgctctatc gcaagattaa gaaatatgag ttgttaaaca
     2461 cataacctca gtgaatccgg cctgaattca ggccggatat ccttttactt cgttttctcg
     2521 caatccagaa ctgtatttac aatcatctga taccccgtgc agcgacaaag atttcccgcc
     2581 agtccgcgac gaatttccgt aatggttaat ggcttctcgc gtggtttcgc cagcattgcc
     2641 gtggtagcca taatcaggcc aggcgtacaa aacccgcact gcactgcgcc ggatttcgca
     2701 taagcctgct gaacatgaga aagttttccg cctttcgctt caccttccag cgtgcggatc
     2761 tcttttcctt cagcccaggc ggcaaggtat aagcaactgt ctattgctgt gccgtcgacc
     2821 aacaccgtac aggcaccaca ttcacccacg cagcaccctt gtttgacact tagcagtcct
     2881 tgttcgcgga gtaattccga gagcggcgtg cctggtgcgg cgtgaagctg aaaaggcatc
     2941 ccgttaatgg tgcattcgat ggtaattgtt tcgctgtgat tcattgcaat tttccccccg
     3001 ccgcggcgac ggcttcgcta atcacttttt tggtcatcgt ctggatgaga tgcagacgaa
     3061 actctttact ggcccgccat gaagaacgcg gggcgacatc ttgcaggaca gattcgctga
     3121 tagcttccag cgtttgcagg tttaatggcg cattttgtgc agtctgttcg gcatgttggc
     3181 agcgaatcgg cgttggcgcg gcaacaccaa atgccaggcg taattcgctg aaattgccgt
     3241 tatccagtcg gcaatgtgcg gcgcagccaa tcgttgaaat atccattgcg tcgcgcatgg
     3301 catatttaaa atgcgcgctg cccgcgtgtt ctttcggctg tggcggaaaa tgaaaggcga
     3361 cgaggatttc gtcatgctca agagacactt tgcccggccc ggtgtgaaag ccattaatcg
     3421 ggacgaaacg aacaccgcgc ggggagtgga tctccagttt cgcgtcataa attagcgttg
     3481 gcgtggcaga atctgcgctg gtggcaccgt tgcaaatatt tccaccgtag gtagcgacgt
     3541 tacggatctg cagtccagca atggacatgg ccgcagcaca taacgccggg agatgacgtt
     3601 gagttatagg atcttctatt agctgggtaa atgtcgttgc agagccgata cgtagcgagc
     3661 catcttccgc cagcgtaatt ccccgcagct ccgccagatt atgaatatca acaatatggc
     3721 gataacggtc attgtgatgg tggagctgaa tcagtacgtc agtgccaccg gcgagcagtt
     3781 tggcctgcgg gttgttagcc agcaggttga tggcatcggc aagggttgct gcgcgatggt
     3841 aagaagcaaa atcaaacatg atgttatcct caaatcaatc ctgccagatg gaactcttca
     3901 tataaccgtt ttggcgtcag cggcagtgta ttgattgcaa caccggtagc catcttcacc
     3961 gcgttacgaa tagcagcggc aacaggaatt attggtggct cacccagtga cttatgtccg
     4021 tatgcggatt gcggctcatt gatttcgacg aacgcgcttt ccagttgtgg cagatccggc
     4081 atggtcggca ttttgtaatc cagcagattg gggttacgga ccacgccgct tttagcatcg
     4141 atgatcatct cttcaaatag cgcccagcca atgcccattc ccattccgcc gtgtacctga
     4201 ccttctgcca gcagtggatt aagaatatgc cctgaatcat gaacgttgag gatgcggttg
     4261 atggtgactt tgcacagcgc aatatcgacc gtcagatcaa caaaggtaca gccaaacgcc
     4321 ggtgggttag tggtggtttt gatggagctt tcagcagaga gctgcccgcc gcgttcaggg
     4381 tggtagaaag cgtccatcgc caaatctttt aacgacatta acggctcttc cggtcgttca
     4441 accagcacga tatggccttt tatcagggtc agattcatcg ctgactgatg tagcatgact
     4501 gcggcgtgag cgatgatttt ctcttttaat aatagtgccg cactgcgcag cgcaggcgcg
     4561 gcaacatagc tctggcgtga ggcaaatgtg ccgggatcga acggcgtaac gtcggtatct
     4621 tgagttgaaa taacgcgaac gtcgctgacc ggaaccccca cggtttctgc caccatttgc
     4681 gagaagacgg tgtcggcacc ctgaccgatt tccgtcgcgc cgctttgcac gttgatggtt
     4741 ccatcctgat tcatcagaag gcgcgcgcct gctatttcta cgccgacagg ccaggtgtta
     4801 gaggtgtagc taaaacaggc gacgccaacg ccgcggcgca aattgccttg ctggttctgg
     4861 cattctgcac ggcgtttttc ccattcaaag attttccggc ctttttcaag acactccggc
     4921 aaccctgcgc tgtaaatacg tttgcccgtg agcggattag catctccttc gcgggcggcg
     4981 ttgcgtaaac gaatttcaac aggatcaata cctaacgctg tcgcggcgtc atcaagcata
     5041 gactcaacgg caaatacgac ttgtggcgcg ccataaccac gcatcgcacc agccgagggg
     5101 aggttggtat agcaggtctt tgaactgtaa gcgtaggcac aacgaggata aaggtaagcg
     5161 actttattcc cccccgcaga agcgatggag tgcccgtgag atgcataagc gccggtgtta
     5221 gacagaacat ccagactata acctttcaat gttccgtcgc ggttcacgcc catttgcccg
     5281 tcaatggtaa aagcgtggcg ggtacgggtt gcgaggaaac actcttcacg gctaagggaa
     5341 actttcaccg gaatgccgcc aagcttgctg gtcaggaatg ccgccattgg ctcttccagt
     5401 acatcctgtt tattaccaaa accgccaccg acaaatggtt tgatgactcg tacgcatgac
     5461 cagggaatat ccagcgcctg accaaccacg cggcgaacaa tgtgcgggat ctgggtgctg
     5521 gaaacgatgg taattcgcga gtcatcctcc atccacgcca gcgatgttac gctttccatg
     5581 tgacaatgtt gaataacggg ggtctgatag tgcccctgta cctggtagtc ggcggcatcg
     5641 attgtttgtt ggacattacc cgtcgacatc gtgctttgtt tcagtaaatt gccaccgtta
     5701 tggattggtg cagcgtcttc tgccagcgcc gcttctggcg tggtgataac gggtaattct
     5761 tgccactcaa tgctgaccaa ttgcgccgct ttttctgccg tgagttcatc gcgggccacg
     5821 acgatggcaa tggcgtcacc atgatgacga acatggcgag ttagcagtgc gcgatcggcg
     5881 gtatcgcgct tgttttcgtc aagtgtccag gcatgcccag ctgtagcgaa tggaatatca
     5941 ggcacatctt cccaggtaaa aatcgccagt acgcccggca aacttctggc ttgttcatca
     6001 ttaatactta cggcataacc atgtgcgata gggctacgta catatttcgc ataacacatg
     6061 cccgccataa cataatcgtc agtatatcgt gcccgcccgg tgaccttagc aatggcatcg
     6121 acgcgcatgc atgattcacc cgtagcggtt gcttcccgcg cttccataga aatcccctcg
     6181 atttgaacat ttggttctta tggcaaagat ttagtctaag caggcaagat ttacgccaga
     6241 aacgacgata agcgttaggg gaggtaggaa gtgtgatgac gttaaaaaaa cacagatatg
     6301 atacgaaaca ggagtctgaa taggggcagt gatacgtatc aatatgagtg gcgataaaat
     6361 caatgtgata aattgggatt attacgcatc agg
//
LOCUS       DAJTOR010000205         6309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTOR010000205 DAJTOR010000000
VERSION     DAJTOR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.205"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            275..616
                     /gene="raiA"
                     /locus_tag="OO249_003726"
     CDS             275..616
                     /gene="raiA"
                     /locus_tag="OO249_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5637855.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    719..844
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            720..767
                     /gene="pheL"
                     /locus_tag="OO249_003727"
     CDS             720..767
                     /gene="pheL"
                     /locus_tag="OO249_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR5637856.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            867..2027
                     /gene="pheA"
                     /locus_tag="OO249_003728"
     CDS             867..2027
                     /gene="pheA"
                     /locus_tag="OO249_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5637857.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2070..3191)
                     /gene="tyrA"
                     /locus_tag="OO249_003729"
     CDS             complement(2070..3191)
                     /gene="tyrA"
                     /locus_tag="OO249_003729"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5637858.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLKQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3202..4272)
                     /gene="aroF"
                     /locus_tag="OO249_003730"
     CDS             complement(3202..4272)
                     /gene="aroF"
                     /locus_tag="OO249_003730"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5637859.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDLETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4480..4845
                     /locus_tag="OO249_003731"
     CDS             4480..4845
                     /locus_tag="OO249_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5637860.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4995..5513
                     /locus_tag="OO249_003732"
     CDS             4995..5513
                     /locus_tag="OO249_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5637861.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAKQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5503..>6309
                     /gene="dgcN"
                     /locus_tag="OO249_003733"
     CDS             5503..>6309
                     /gene="dgcN"
                     /locus_tag="OO249_003733"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCR5637862.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINT"
BASE COUNT         1712 a         1610 c         1418 g         1569 t
ORIGIN      
        1 agtcagttgc ttaactgact ggcattacag cccttgagct gccgtttttt tattctgtta
       61 gttgtgaaac tgaagcgatt tagtcactat cgatctcatc aaatatggct cgctttgaga
      121 tattcctcaa gtaaaaaaac acctcttcct gcgatttctc acaaaaaaga ttcgttgaca
      181 aaaagtgaca aaattatgag attcccatca cacattttga catcaggaac ggtatgctga
      241 attcaccaag acgggaagac aagaggtaaa atttatgaca atgaacatta ccagcaaaca
      301 aatggaaatt actccggcca tccgccaaca tgtcgcagac cgtctcgcca aactggaaaa
      361 atggcaaaca catctgatta atccacatat cattctgtcc aaagagccac aagggtttgt
      421 tgctgacgcc acaatcaata cacctaacgg cgttctggtt gccagtggta aacatgaaga
      481 tatgtacact gcaattaacg aattgatcaa caagctggaa cggcagctca ataaactgca
      541 gcacaaaggc gaagcacgtc gtgccgcaac atcggtgaaa gacgccaact tcgtcgaaga
      601 agttgaagaa gagtagtcct ttatattgag tgtatcgcca acgcgccttc gggcgcgttt
      661 tttgttgaca gcgtgaaaac agtacgggta ctgtactaaa gtcacttaag gaaacaaaca
      721 tgaaacacac accgtttttc ttcgcattct tttttacctt cccctgaatg ggaggcgttt
      781 cgtcgtgtga aacagaatgc gaagacgaac aataaggcct cccaaaccgg ggggcctttt
      841 ttattgataa caaaaaaggc aacactatga catcggaaaa tccattactg gcgctgcgag
      901 agaaaatcag cgcgctggat gaaaaattat tagcattact ggcagagcgg cgcgaactgg
      961 ccgtcgaggt gggaaaagcc aaactgctct cgcatcgccc ggtacgagat attgatcgtg
     1021 aacgcgattt actggaaaga ttaattacgc tcggtaaagc gcaccatctg gacgcccatt
     1081 acattactcg cctgttccag ctcatcattg aagattccgt attaactcag caggctttgc
     1141 tccagcaaca tctcaataaa attaatccgc actcagcacg catcgctttt ctcggcccca
     1201 aaggttctta ttcccatctt gcggcgcgcc agtatgctgc ccgtcacttt gagcaattca
     1261 ttgaaagtgg ctgcgccaaa tttgccgata tttttaatca ggtggaaacc ggccaggccg
     1321 actatgccgt cgtaccgatt gaaaatacca gctccggtgc cataaacgac gtttacgatc
     1381 tgctgcaaca taccagcttg tcgattgttg gcgagatgac gttaactatc gaccattgtt
     1441 tgttggtctc cggcacgact gatttatcca ccattaatac ggtctacagc catccgcagc
     1501 cattccagca atgcagcaaa ttccttaatc gttatcctca ctggaagatt gaatataccg
     1561 aaagtacgtc tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt
     1621 tgggaagcga agctggcggc actttgtacg gtttgcaggt actggagcgt attgaagcga
     1681 atcagcaaca aaacttcacc cgatttgtgg tgttggcacg taaagccatt aacgtgtctg
     1741 accaggttcc ggcgaaaacg acgttgttaa tggcgaccgg gcaacaagcc ggtgcgctgg
     1801 ttgaagcgtt gctggtgctg cgcaaccaca atctgattat gacccgtctg gaatcacgcc
     1861 cgattcacgg taatccatgg gaagagatgt tttatctgga tattcaggcc aatcttgaat
     1921 cagcggaaat gcaaaaagca ttgaaagagt taggtgaaat tacccgttca atgaaggtat
     1981 tgggctgtta cccaagtgag aacgtagtgc ctgttgatcc aacctgatga aaaggtgccg
     2041 gatgatgtga atcatccggc actggattat tactggcggt tgtcattcgc ctgacgcaat
     2101 aacacgcggc tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg
     2161 cggaaactgt caataaacgc ctgcttatcg ccctgcttca gcaactcaat cgcctcgccg
     2221 aaacgcttat agtaacgttt gattaacgcc agattacgct ctgacgacat aataatgtcg
     2281 gcataaagct gcggatcctg agcaaacagt cgcccgacca tcgccagctc aaggcggtaa
     2341 atcggcgaag agagcgccag aagttgctca agctgaacat tttcttctgc cagatgcagc
     2401 ccataagcaa aagtagcaaa gtggcgcaga gcctgaataa acgccatatt ctgatcgtgc
     2461 tcgacagcgc taatacgatg cagccgagcg ccccagacct gaatttgctc cagaaaccat
     2521 tggtatgctt ccggcttacg tccatcacac cagaccacaa cttgctttgc caggctaccg
     2581 ctgtccgggc cgaacatcgg gtgtaacccc agtaccgggc catcgtgcgc cgccagcatg
     2641 gcctgtaatg gtccattttt cactgatgcc agatcaacca gaatacaatc tttcggtaaa
     2701 ggcggtaatt tgccgataac ttgctcagta acgtggattg gcacactaac aatcaccatt
     2761 ccggcatcgg caacaatatc agccgctcga tcccagtcat gttgctccag aatccgcacc
     2821 tgataacccg agagtgtcag catcttctcg aacaggcgtc ccatctgacc gccaccgccg
     2881 acgataacca ccggacgcag tgacggacaa agtgttttaa atcctttgtc gttttcactg
     2941 gagtaagatt cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg cggtaccccc
     3001 agagcttccg cctctgcacg acgcgaggcc aacatagatg cctcgcgctc cggaacataa
     3061 ataggcagtc caaagcggct tttcacctcg cccacttcag caaccagttc cagacgcttc
     3121 gctaataaat tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa tgcggtcaat
     3181 tcagcaacca taataaacct cttaagccac gcgagccgtc agctgcccgt tcagatcctg
     3241 atgaatttca cgcagcaagg catcggtcat ttcccagcta atgcaggcat cggttacgga
     3301 tacaccgtat ttcatttcac tgcgcggttg ctcagaagac tgattgccct cgtggatatt
     3361 actttcgatc atcagaccaa taattgagcg attgccatct ttgatttgag caaccacgga
     3421 ttctgccacc gcaggctgac ggcgataatc tttattggaa ttaccgtggc tgcaatctac
     3481 catcagagac gggcgcagtc ccgcctgttc catctctttt tcacattgcg caacatccgc
     3541 agggctatag ttcggcgctt taccaccgcg caggatgaca tgcccgtccg gattcccctg
     3601 agtttgtagc aacgcaacct gccctgcctg gttaatgcca acaaaacggt gcggctgggc
     3661 ggcggcgcgc atagcgttaa ttgctgttgc cagactgccg tcggtgccgt ttttaaaacc
     3721 aaccggcatg gaaagcccgg aggccatttc acggtgagtt tgcgattccg ttgtacgagc
     3781 accaattgct gaccagctaa acagatcgcc caggtattgc gggctattcg gatctaacgc
     3841 ttccgtcgcc agtggcagtc ccatattcac cagctcaagc agcaatttac gcgcgatctg
     3901 cagcccggct tctacatcaa aagagccatc catatgggga tcgttaatta accctttcca
     3961 gccgacagtg gtacggggtt tttcaaaata gacgcgcatt accagataga ggctatcgct
     4021 gacctctgcg gcaagggctt taaatcgacg agcatattcc agagcagttt ccagatcatg
     4081 aatggaacaa ggaccacaaa ctaccagcag acgaggatcg cgcccggcga taatatctga
     4141 aatggtttta cgcgagtcag caatctgggc ttcttgttgc aggctcaatg gaaaagcggc
     4201 cttcagttgt tccggagtca ttaaaacctg ttcgtcggta atatgtacgt tattcagcgc
     4261 gtctttttgc atgatggcga tcctgtttat gctcgtttgc gatagttgat cctcagcgag
     4321 gatgacgtaa cgataacaca taaagtaaag ttttcaatcc atatttcgta cgcttttctt
     4381 tacacgaaca atttactcgc gctttgatgc ttatttcgta caaataaatt tacaccccct
     4441 ttaaaaacaa tccgctatgc tttgaaaaag gagaaagaaa tgatgaaaaa gtttatcgcc
     4501 cccttattgg ctttactggt tagcggatgt cagattgatc cttatactca cgcgccaacc
     4561 ttgaccagca ccgactggta tgatgtcggt atggaagatg cgatatcggg cagcgccata
     4621 aaagatgacg atgcatttgg cgattcacag gcggatcgcg gtctatacct taaaggatat
     4681 gccgaaggac aaaagaaaac ttgccagacc gattttactt atgcccgggg actttccggt
     4741 aaaagctttc ctgccagctg taacaatgtt gaaagtgcca gccaactgca tgaagtatgg
     4801 caaaaagggg ctgatgaaaa tgccagcaca atacgattaa attaattaaa accccacaaa
     4861 taaattaaac ataagatttt actcattgtg ttttatttct cacattgatg acgatcgcat
     4921 gaaaaaatga tattttaatg agcattctga aaaaatatcg atgcatttcg agcgaagaag
     4981 gtggggatcc ctgaatgcgt ttttctcacc gactgttctt acttctgatt ctcctgctga
     5041 cgggagcacc aatactcgct caggagcctt ctgacgttgc gaaaaacgtg cgtatgatgg
     5101 tttccggtat tgtcagttac acccgctggc ctgcgctatc agggccacca aagttatgta
     5161 tcttttcatc ttcacgtttt agtaccgccc tacaagagaa tgccgcgaca tctttgcctt
     5221 atctgcctgt catcattcac actcaacaag aggcgatgat ttcaggctgc aatggttttt
     5281 attttggcaa cgagtcaccg acattccaga tggaattaac cgaacaatat ccgtcaaagg
     5341 cgttattatt aattgccaaa caaaataccg agtgcattat tggtagcgcc ttttgtctga
     5401 tcatccacaa taatgacgtc agatttgccg taaacctgga tgccttatcg cgtagcggtg
     5461 taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa aaatgatgga taacgataat
     5521 tctcttaata agcgccccac gtttaaaaga gcattacgca acatcagtat gaccagcata
     5581 tttatcacta tgatgctgat ctggttgctg ctttccgtga cctcggtgct gaccctgaaa
     5641 cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa tgacttacag tctggaagca
     5701 gctgtcgttt ttgccgatgg ccctgcagca actgaaacac tggcagcgct gggccagcaa
     5761 gggcaatttt caactgcaga agtacgtgat aagcagcaaa atattctggc gtcctggcat
     5821 tacacccgta aggatccagg cgatactttc agcaatttca taagccactg gctcttcccc
     5881 gcccccatca ttcagccgat tcgtcacaat ggtgaaacca ttggcgaagt acgcttaacc
     5941 gctcgcgaca gttcaatcag ccattttatc tggttttcgc tcgccgtact gaccggttgt
     6001 attctgctgg catcaggcat cgcaattacc ctcacccgcc atttgcacaa tggcctagtg
     6061 gaagcgctga aaaatatcac cgatgtcgta catgatgtgc gttccaaccg caatttttcc
     6121 cgacgagttt ctgaagaacg tatcgctgag tttcaccgct tcgctctcga cttcaacagt
     6181 ctgctggatg aaatggaaga gtggcagctt cgtttacagg ccaaaaatgc gcagcttctg
     6241 cgtaccgcgc tacatgaccc attaaccggg ctggctaacc gcgcagcgtt tcgtagcggt
     6301 atcaatacg
//
LOCUS       DAJTOR010000206         6294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTOR010000206 DAJTOR010000000
VERSION     DAJTOR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.206"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(86..622)
                     /gene="ssb1"
                     /locus_tag="OO249_003734"
     CDS             complement(86..622)
                     /gene="ssb1"
                     /locus_tag="OO249_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5637863.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            876..3698
                     /gene="uvrA"
                     /locus_tag="OO249_003735"
     CDS             876..3698
                     /gene="uvrA"
                     /locus_tag="OO249_003735"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5637864.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3733..4089)
                     /locus_tag="OO249_003736"
     CDS             complement(3733..4089)
                     /locus_tag="OO249_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5637865.1"
                     /translation="MTISELLQYCMAKLGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLRPEEKRKLLVQL"
     gene            complement(4093..4509)
                     /locus_tag="OO249_003737"
     CDS             complement(4093..4509)
                     /locus_tag="OO249_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5637866.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLANMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4620..5333)
                     /gene="aphA"
                     /locus_tag="OO249_003738"
     CDS             complement(4620..5333)
                     /gene="aphA"
                     /locus_tag="OO249_003738"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5637867.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5735..5957
                     /locus_tag="OO249_003739"
                     /pseudo
     CDS             5735..5957
                     /locus_tag="OO249_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1593 a         1556 c         1568 g         1577 t
ORIGIN      
        1 ccataacgga ggcttctttt ttatggctgg cagatgcttt aatcatccac cttaaaacaa
       61 tataacctat tgttttaatg acaaatcaga acggaatgtc atcatcaaag tccatcggcg
      121 gctcgttaga cggcgctgcc ggagcggact gctgcgggcg agactgcgcg ccgccgctga
      181 actgattgcc accctgcggc tgctgaggct gaccccaacc gccctgcggc tgaccaccac
      241 cgatattacc acctgccgga gcgccaccac cctgacgacc acccagcatc tgcatggtgc
      301 cgccaacgtt caccacgact tctgtggtgt agcgatcctg accggattga tcggtccatt
      361 tacgggtacg cagctgacct tcgatataaa cctgagaacc tttacgcaga tattcgctgg
      421 ccacttctgc cagtttgccg aacagcacaa cgcggtgcca ttcagtctgt tctttcatct
      481 cgccggtcgc tttatcacgc caggattcgg aagtagccag cgtaatgttg gcaactgcgc
      541 caccatttgg catgtagcgt acttccgggt cctgacccag attaccaacg agaataacct
      601 tgtttacgcc tctgctggcc atgttcgtgt ctcctgaaaa aaatcgttct gaataagtgt
      661 aaacgcgcga ttgtaccatt accaatagcg cttttactat gttgtgacct cggttccggg
      721 aaacaaacct ggccagacat tgttacacaa cactccggat aatgcattcc aatactgtat
      781 attcattcag gtcaatttgt gtcataatta accgtctgtg atcgccggta gcaccatgcc
      841 accgggcaaa aaagcgttta atccgggaaa ggtgaatgga taagatcgaa gttcggggcg
      901 cccgcaccca taatctcaaa aacatcaacc tcgttatccc ccgcgacaag ctcattgtcg
      961 tgaccgggct ttcgggttct ggcaaatcct cgctcgcttt cgacacctta tatgccgaag
     1021 ggcagcgccg ttacgttgaa tccctttccg cctacgcgcg gcagtttctg tcactgatgg
     1081 aaaagccgga cgtcgatcat attgaggggc tttctcctgc catctcaatt gagcagaaat
     1141 cgacgtctca taacccgcgt tctacggtgg ggacaatcac cgaaatccac gactatttgc
     1201 gtttgttgtt cgcccgcgtc ggcgaaccgc gctgtccgga ccacgacgtc ccgctggcgg
     1261 cgcaaaccgt cagccagatg gtggataacg tgctgtcgca gccggaaggc aagcgtctga
     1321 tgctactcgc gccaatcatt aaagaacgca aaggcgaaca caccaaaacg ctggagaacc
     1381 tggcaagcca gggttacatc cgtgctcgta ttgatggcga agtctgcgat ctttccgatc
     1441 cgccgaaact ggaactgcaa aagaaacaca ctattgaagt ggtggttgat cgcttcaagg
     1501 tgcgtgacga tcttacccaa cgtcttgcgg agtcgtttga aaccgcgctg gagctttccg
     1561 gtggtacagc ggtagtggcg gatatggacg acccgaaagc ggaagagctg ctgttctccg
     1621 ccaacttcgc ctgcccaatt tgcggctaca gtatgcgtga actggagcca cgactgtttt
     1681 cgtttaacaa cccggcaggt gcctgcccaa cctgtgacgg ccttggcgta cagcaatatt
     1741 tcgatcctga ccgcgtgatc caaaaccccg agctgtcact ggctggcggt gcgatccgtg
     1801 gctgggatcg ccgcaacttc tattacttcc agatgctgaa atcgctggca gatcactata
     1861 agttcgacgt cgaagcgccg tggggcagcc tgagcgcgaa cgtgcataaa gtggtgttgt
     1921 acggttctgg caaagaaaac attgaattca aatacatgaa cgatcgtggc gatacctcca
     1981 tccgtcgtca tccgttcgaa ggcgtgctgc acaatatgga gcgccgttat aaagagacag
     2041 aatccagtgc ggtacgtgaa gaattagcca agtttatcag caatcgccca tgcgccagct
     2101 gcgaagggac gcgtctgcgt cgggaagcgc gccacgttta tgtcgagaat acgccgctgc
     2161 ctgctatctc cgacatgagc atcggtcatg cgatggaatt cttcaacaat ctcaaactag
     2221 ctggtcagcg ggcgaagatt gcagaaaaaa tccttaaaga gatcggcgat cgtctgaaat
     2281 tcctcgttaa cgtcggcctg aattacctga cactttcccg ctcggcagaa acactttccg
     2341 gcggtgaagc ccagcgtatc cgtctggcga gccagattgg tgcgggcctg gttggcgtta
     2401 tgtatgtact ggacgagccg tctatcggcc tgcaccagcg cgataacgag cgcctgttgg
     2461 gtacgcttat ccatctgcgc gatctcggta ataccgtgat tgtggtggag catgacgaag
     2521 acgcaattcg cgccgctgac catgtgatcg atatcggtcc tggtgcgggt gttcacggcg
     2581 gtgaagtggt cgcagaaggt ccgctggaag cgatcatggc ggtgcctgaa tcgttgaccg
     2641 ggcagtacat gagcggtaaa cgcaagattg aagtgccgaa gaaacgcgtt ccggcgaatc
     2701 cagaaaaagt gctgaagctg acaggtgcac gcggcaacaa cctgaaggac gtgacgctca
     2761 cgctgccagt cggtctgttt acctgcatca caggggtttc aggttccggt aaatcgacgc
     2821 tgattaacga cacactgttc ccgattgccc aacgccagtt gaatggtgcg accatcgccg
     2881 aaccggcacc gtatcgcgat attcaggggc tggagcattt cgataaagtg atcgatatcg
     2941 accaaagccc aattggtcgt actccacgtt ctaacccggc gacctatacc ggcgtgttta
     3001 cacctgtgcg cgaactgttt gcgggcgtac cggaatcccg tgcgcgtggt tatacgccag
     3061 gacgtttcag ctttaacgtc cgtggcgggc gctgcgaagc ctgtcagggc gacggtgtga
     3121 tcaaagtgga gatgcacttc ctgccggata tctacgtgcc gtgcgatcag tgcaaaggta
     3181 aacgctataa ccgtgaaacg ctggaaatta agtacaaagg caaaaccatc cacgaagtgc
     3241 tggatatgac catcgaagag gcgcgtgagt tctttgatgc ggtgccagct ctggcgcgta
     3301 agctgcaaac gttgatggac gttggtctga cgtacattcg cctggggcag tccgcaacca
     3361 cactttctgg tggtgaagcc cagcgcgtga agctggcgcg tgagctgtca aaacgcggca
     3421 ccgggcagac gctgtatatt ctcgacgagc cgaccaccgg tctgcacttc gccgatattc
     3481 agcaactcct cgacgtgctg cataaactgc gcgatcaggg caataccatt gtggtgattg
     3541 agcacaatct cgacgtgatc aaaaccgctg actggattgt cgacctggga ccagaaggcg
     3601 gcagtggcgg cggcgaaatc ctcgtctccg gtacgccaga aaccgtcgcg gagtgcgaag
     3661 cttcgcatac ggcacgcttc ctcaagccga tgctgtaatc gttaaggccg ctttctgagc
     3721 ggccttttcc tttcagagtt gtaccagcaa tttacgcttt tcttccggtc gtaaattcac
     3781 tgcctgctga taagacgcat ccaccagata atagatttgc gaatccggca gcgaaccgtc
     3841 gagatacacg gtgctccagt gcgctttatt cagatggcgg ctaggacgca catcgctgtg
     3901 ctgctgacgt agcagctccg ccagctccgg gctggttttc agcgaaacag ctgggcgatt
     3961 ttctacttct ttcaccatcg caaacagtac atcttccact ttgatctgcg tcgctttcca
     4021 gtcgttatgc acgctctgtt ctgcgcctag ttttgccatg caatattgta gcaactccga
     4081 aatggtcatt ttttactccc cttgtagtgt cgcgatgatg cgacgcgatc cgccgtggat
     4141 gcgatgttcc cccagccaga tgccttgcca ggtgccggtc tgaatacgcc ctttatgtaa
     4201 cggcaataca agcgatgttc cctgcattga ggatttgata tgagaaggca tatcgtctgc
     4261 tccctcatag tcatgctcat aatttccgtt gtcgggaacg gtgcggagga aaaaacgctc
     4321 catgtcgtgg cgtacggtgg gatcgcagtt ctcattaagt gtcagagagg cggaggtatg
     4381 ttgcagcaac agatgcagta agccgatgtt aacgcgcggc atattagcca gctgattcag
     4441 aatttcatcc gttaccagat gaaacccacg agatttggcg ctaagcgtga gcgtcttttg
     4501 ataccacatg tgctgctcct tgataaaact ctcttaatca gtttgcagca agacagcgaa
     4561 aggataaagg tgtgattaaa aaaacagcat tcgggagagc atcacgctct cccgctctgt
     4621 cagtattctg aattgacgat cacctcttca ccaaacgcac ccgcttgtgg caagggtttg
     4681 taggtagagt tggaggcgcg cagaatgcgg ataccacgag cgccgacatc gcgtgcggcg
     4741 gtaatatcat tatcagaatc gccataaaaa attcggatat ttttatcctg cagccattgc
     4801 gattttgtat tttgccctgg tttatcgccc gcaaagatca ccggattcat gttggtggca
     4861 ggaatatgaa aattatccgc cagcgttttt gaaaccgttt ctgttttcgt cggactacga
     4921 ccagtcacaa agaagatcgc gtcaccgcgg cgtacatgca tatcaatcag ctggcgagcg
     4981 acctcttttg gaatgctgaa ttcatcccag ccattgttca ttttttccca gaacacagga
     5041 tttttcagat aatcttcgct ttctggcgag aaggtttttt tgccgcgcca gaagcccggg
     5101 ctggaaaaaa gtaccgtgtc atcgatatca aaccccaccg ccattggcgg acgccctgcg
     5161 aggctatttt caatttgtgc gaccgaaacc caatgaattg gtgcctgttc agcaagcctg
     5221 gcaacgttag taccagggtt aagcggtgaa ggagatgagg ccagggcaac agcggaactg
     5281 tttagagcga acaataagca aacggcactg attgcctgtg tgatcttgcg catatttttc
     5341 cctaaatagt cagtatgttg aaacttttta ttgtgagatt tgttgcaaaa accatctgac
     5401 cataacgcca gctgcagggg aaaggaagga ttatctgcgg tttttgtgat gaaaagcagg
     5461 atgattatat ttaacccagt aaatacgttc ctgtcttttt ttgtataaaa aatgtttatt
     5521 actcgatgta aggatacatt aattttacgt ttattatgat gaatcctctg caaatgagcg
     5581 aaatttgata gaacatttat ctgctgtacg atttaatgaa cttaatgata cagcattaca
     5641 aaaacaatcg aagtttataa agatgattcc tgattgacca gaaccctatc ctacaatggt
     5701 gattaatttt attactcgcg atggagatga tgatatgaat atatcatata tgaatagcaa
     5761 caaaacgaca tctttacctg ttgagctaga tgcactaaat aataaagata tctcttatgc
     5821 caaagatttt tcttatgtca aagatttctt tttatacata gaaacacagt taaaaattgc
     5881 aaaagatttt tgtagacttg gagaagaagt atcaagttct attgcaagta aagtttttca
     5941 cgcatttatt gatttagtta acaaaatcag gggtaagaaa gactttatgt atatttgcac
     6001 gctttgctgt tttgccgagg aggttaaagg tgattattct cattacagga cctttttatt
     6061 tgatattggt aatcaatata aggttaaact tacacaaagc ggaaaaaaag agttctcttt
     6121 aacattagaa tttaacgata ctataattga atctcagaaa gtcacaggca ataaggcaaa
     6181 gcatattctt gaggatatag aaatattcta tcgtaacaaa cccgatactt attattaaat
     6241 ttaataatgg ttagatgtgc agaaattatt actccacacg aaaaaatagg gaaa
//
LOCUS       DAJTOR010000207         6052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTOR010000207 DAJTOR010000000
VERSION     DAJTOR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.207"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..236)
                     /gene="yneK"
                     /locus_tag="OO249_003740"
     CDS             complement(<1..236)
                     /gene="yneK"
                     /locus_tag="OO249_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5637868.1"
                     /translation="MVTPISISNYIFLPDDFPVRNIAPQVKEVLKNFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDT"
     gene            complement(314..1195)
                     /locus_tag="OO249_003741"
     CDS             complement(314..1195)
                     /locus_tag="OO249_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5637869.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTIRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            1296..2684
                     /gene="sad"
                     /locus_tag="OO249_003742"
     CDS             1296..2684
                     /gene="sad"
                     /locus_tag="OO249_003742"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCR5637870.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2748..3674
                     /gene="glsB"
                     /locus_tag="OO249_003743"
     CDS             2748..3674
                     /gene="glsB"
                     /locus_tag="OO249_003743"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5637871.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3674..4033
                     /locus_tag="OO249_003744"
     CDS             3674..4033
                     /locus_tag="OO249_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5637872.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            4172..5590
                     /locus_tag="OO249_003745"
     CDS             4172..5590
                     /locus_tag="OO249_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5637873.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            5817..>6052
                     /locus_tag="OO249_003746"
     CDS             5817..>6052
                     /locus_tag="OO249_003746"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5637874.1"
                     /translation="MKTLNRRDIPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNE"
BASE COUNT         1440 a         1395 c         1619 g         1598 t
ORIGIN      
        1 gtgtcgttac cccgaaaaga agtcctctga atatatgata agttatcaag gttgttaaat
       61 atcttttttg tggctgagtt ggtgtttaaa gacgtgcgcc actcttcatc acatattatg
      121 gtactgagtg catcaataaa attctttaaa acttctttta cctgaggtgc aatattacgt
      181 acgggaaaat catcaggaag aaatatgtaa ttactgatgc ttattggcgt aaccatattt
      241 gccccatata gtcataagtc atcggaacat cactatatag ggcatttgag ctgcaagaac
      301 tatcaaaaat agctcattgg taaccctgtt ttgccgagtc aggcacatcc agtagttgaa
      361 taaatgcctc aagcggacgc gttttcgcac cacgacgcca gaccagccag gttgttaacc
      421 aacgccattg ctcagctaac ggccacgctt caacctggtg atgccccggc atactttcca
      481 gcatagagcg tggaataagc gcaatgcctg ctcctgcgac cacacaggcc aacatcccgt
      541 gatacgactc catctcgtga atagttcccg gagcggcacc gtcagcatga aaccagctct
      601 cgaagtggcg acgatacgaa caattggcgc ggaaggcata aatgttactg ccgttaacct
      661 gactggcgcg gattactggc gcatgacctt gtggcgtgac gatcatcagc tcctcgcggt
      721 ataccggtat cccgtcaatg gcagtatggt taatgggccc atcaataaat gccgcattca
      781 gttttccctc cagcactcct tccagcatag tgccggaagg gccggtggaa agtgaaaact
      841 gaattttggg ataacgatgg ttgaattccg ccagcgtggc cggaatgcgc actgctgcgg
      901 tgctttccag cgaaccaaga gaaaataaac cttgcggctc atcgccagcg acaacgctcc
      961 gcgcttcatc cactaacgcg agaatttgtt ggctatagcg taaaaagtta tgcccggcag
     1021 gagagagacg taaacgctga ttctcgcgaa taaacagatc tacccccagt tctgtttcca
     1081 gctggcgtag acggatagtg agattcgacg gcacgcgaag cactattgct gctgcctggg
     1141 ttatgctgcc agcttcggca accgcgttga acatctccag ttgggtcaga tccataatat
     1201 tctcgattcg tgaataagtg gcttaatatt attcatttta aagcaagagt aaatctgcgt
     1261 atcttcatac catgactcat aaaggagata ccccgatgac cattactccg gcaactcatg
     1321 caatttcgat aaatcctgcc acgggtgaac aactttctgt gctgccgtgg gctggcgcta
     1381 acgatatcga aaacgcactt cagctggcgg cagcaggctt tcgcgactgg cgcgagacaa
     1441 atatagatta tcgtgctgaa aaactgcgtg gtatcggtaa ggctctgcgc gcccgtagcg
     1501 aagaaatggc gcaaatgatc acccgtgaaa tgggcaaacc aatcaatcag gcgcgcgctg
     1561 aagtggcgaa atcggcgaat ttgtgtgact ggtatgcaga acatggtccg gcaatgctga
     1621 aggcggaacc tacgctggtg gaaaatcagc aggcagttat tgagtatcga ccgttgggga
     1681 cgattctggc gattatgccg tggaactttc cgttatggca ggtgatgcgt ggcgcggttc
     1741 ccatcattct tgcaggtaac ggctacttac ttaaacatgc gccgaatgtg atgggctgtg
     1801 ctcagctcat tgcccaggtg tttaaagatg cgggaatccc gcaaggcgta tatggctggc
     1861 tgaatgccga caacgacggt gtcagtcaaa tgattaaaga ttcgcgcatt gctgctgtca
     1921 cggtgaccgg aagtgttcgt gcgggagcgg ctattggcgc acaggctgga gcggcactga
     1981 aaaaatgcgt actggaactg ggcggttcgg atccatttat tgtgcttaac gatgccgatc
     2041 tggaactggc ggttaaagcg gcggtagccg gacgttatca gaataccgga caggtttgtg
     2101 cagcggcaaa acgctttatt atcgaagagg gaattgcttc tgcatttacc gaacgttttg
     2161 tggcagctgc ggcagccttg aaaatgggcg atccccgtga tgaagagaac gctctcggac
     2221 caatggctcg ttttgattta cgtgatgagc tgcatcatca ggtggagaaa accctggcgc
     2281 agggtgcgcg tttgttactg ggcggggaaa agatggctgg ggcaggtaac tactatccgc
     2341 caacggttct ggcgaatgtt accccagaaa tgaccgcgtt tcgggaagaa atgtttggcc
     2401 ctgttgcggc aatcaccgtt gcgaaagatg cagaacatgc gctggaactg gctaatgata
     2461 gtgagttcgg cctttcagcg accattttta ccaccgacga aacacaggcc agacagatgg
     2521 cggcacgtct ggaatgcggt ggggtgttta tcaatggtta ttgtgccagc gacgcgcgag
     2581 tggcctttgg tggcgtgaaa aagagtggct ttggtcgtga gctttcccat ttcggcttac
     2641 acgaattctg taatatccag acggtgtgga aagaccggat ctgacgcgac atattcagct
     2701 ctgatatact cgcaggtctt ttcagacctg cggtccagga gtagaaagtg gcagtcgcca
     2761 tggataatgc aattttagaa aacatcttgc ggcaagtgcg gccgctcatt ggtcagggta
     2821 aagtcgcgga ttatattccg gcgctggcta cagtagacgg ttcccgattg ggcattgcta
     2881 tctgtaccgt tgacggacag ctttttcagg ccggagacgc gcaagaacgt ttttccattc
     2941 agtctatttc caaagtgctg agtctcgttg tcgccatgcg tcattactcc gaagaggaaa
     3001 tctggcaacg cgtcggcaaa gatccgtctg gatcaccgtt caattcttta gtgcaactgg
     3061 aaatggagca gggtattccg cgtaatccgt ttattaatgc tggtgcgctg gtggtctgcg
     3121 atatgttgca agggcgatta agcgcaccac ggcaacgtat gctggaagtc gtgcgcggct
     3181 taagcggtgt gtctgatatt tcatacgata cggtggtagc gcgttccgaa tttgaacatt
     3241 ccgcgcgaaa tgcggctatc gcctggctga tgaagtcgtt tggcaatttc catcatgacg
     3301 tgacaaccgt tctgcaaaac tactttcatt actgcgctct gaaaatgagc tgtgtagagc
     3361 tggcccggac gtttgtcttt ctggctaatc aggggaaagc tattcatatt gatgaacctg
     3421 tggtgacgcc aatgcaggcg cggcaaatta acgcgctgat ggcgaccagt ggtatgtacc
     3481 agaacgcggg ggagtttgcc tggcgggtgg ggctaccggc gaaatctggc gttggtggcg
     3541 gtattgtggc gattgttccg catgaaatgg ccatcgctgt ctggagtccg gaactggatg
     3601 atgcaggtaa ctcgcttgcg ggtatcgccg tccttgaaca attgacgaaa cagttagggc
     3661 gttcggttta ttaatgcagt ctctcgatcc actcttcgcg cgtttatccc gttcaaaatt
     3721 tcgctctcgc tttcgtctgg gcatgaaaga gcgtcagtat tgcctggaga aaggcgcgcc
     3781 agtcatcgaa caacatgcgg cggattttgt cgctaaacgt cttgctccgg cattaccggc
     3841 taatgacggt aagcaaacac ccatgcgcgg gcatccggtg tttatcgctc agcacgccac
     3901 cgcgacctgc tgtcgcggtt gtctggctaa gtggcataac attccgcagg gtgtttcgtt
     3961 aagcgaagaa caacaacgtt atatcgtggc ggtaatctat cactggctgg ttgtacagat
     4021 gaatcagcca tgagaattgc ggaaaacgat aaaatagcat tcccagggta cctttatttg
     4081 ccgccgctgt tagcgaacat gctatccttt atgagaattt acttattacg ttgagataat
     4141 tgccacccgc aattgtatgg acagataatt aatgcatgta caccccattt caactttccg
     4201 tttgttccag gaaggtcatc tgctacgtaa tagcatcgct atttttgcgc taaccacgct
     4261 gttctatttt attgttgcag agttacggct ggttcacgaa ctttctcttt tctggccgct
     4321 gaatggcgta atggcggggg tgtttgcccg ctatgtctgg cttaatcgac tgcattacta
     4381 tgcgatcagt tatgtggcga tgctggttta tgatgccata accaccgaat ggggactggt
     4441 ttcactggtt atcaatttct ccaatatgat gtttattgtt accgtcgcct tactggtcgt
     4501 gcgggataag cgtctgggga aaaataagta tgagccagta agtgccttac ggctatttaa
     4561 ttactgtctg attgccgcat tattatgcgc tattgtcggg gcgattggtt cggtcagtat
     4621 tgatagtctg gatttctggc ctttgcttgc ggactggttc agtgagcaat tctcaacggg
     4681 cgtgttgatc gtgccttgta tgctgacgtt ggctattcct ggagtgctgc cgcgctttaa
     4741 agcagaacag atgatgcccg ccatcgcgct tattgtgtcg gttattgcct cggtagtcat
     4801 tggcggagcg gggagtctgg cgttcccgct gcctgcatta atctggtgtg cagtgcgcta
     4861 tacgccgcag gtaacatgtc tgttgacctt tgtcaccggt gcggtggaaa tcgtactggt
     4921 ggcaaattcg gtgattgata tctcggtcgg ttcgccgttc tccattccgg aaatgttctc
     4981 cgcacgtctc ggtattgcca cgatggcgat atgcccaatt atggtttctt ttagcgtggc
     5041 agcgatcaat tcgctaatga agcaagttgc gctgcgagcc gactttgatt ttctgactca
     5101 ggtttactca cggtccggtc tttatgaggc gctgaaaagt ccatcgctga aacagacaca
     5161 acatctgacg gtcatgctgc ttgatatcga ctatttcaaa agcattaacg ataactatgg
     5221 acatgaatgt ggcgataaag tgttaagcgt gtttgcccgg catattcaga agattgtcgg
     5281 tgataagggg ctggtggcgc gcatgggcgg cgaagaattt gctgttgcag tgccgtcggt
     5341 gaatcctgtc gatggtctgc taatggcgga aaaaatccgt aaaggcgttg aactgcaacc
     5401 attcacctgg caacaaaaaa cgctttatct cacggtaagt attggcgtcg gtagtggtcg
     5461 cgcatcgtac cgaacgctga ccgatgattt taataaattg atggtcgaag ccgatacgtg
     5521 tctgtatcgc tcgaagaaag atgggcgcaa ccgtaccagc actatgcgtt acggtgagga
     5581 agttgtctga catctttatt cattccgcgc gatttgaatg caatgtgttc taccacttta
     5641 tgatccagga aaaggcgtat ttcctcgatt aaaccatgat ccgcgccaca cttttctcat
     5701 aaaactgctc attaatcatc aggagttgat caaaacaggg gttcggcgta ttaaattttg
     5761 ctaacctgtc gtaccaattc agacgggttg tatgactaat cagaagggaa cccattgtga
     5821 aaacactaaa tcgtcgcgat attcccggtg cacagtatcc agaacgtatc attcagtttg
     5881 gtgaaggtaa cttcctgcgc gcctttgttg actggcaaat cgatctcctg aatgagcaca
     5941 ccgatctgaa ttctggcgtg gtcgttgttc gtccgattga aacttcattc ccgccgtcac
     6001 tgagcacgca ggatggtctg tacaccacca ttatccgtgg cctgaatgag aa
//
LOCUS       DAJTOR010000208         6036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTOR010000208 DAJTOR010000000
VERSION     DAJTOR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.208"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(127..276)
                     /locus_tag="OO249_003747"
     CDS             complement(127..276)
                     /locus_tag="OO249_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637875.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            1465..2280
                     /gene="sul2"
                     /locus_tag="OO249_003748"
     CDS             1465..2280
                     /gene="sul2"
                     /locus_tag="OO249_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5637876.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2367..2669
                     /locus_tag="OO249_003749"
     CDS             2367..2669
                     /locus_tag="OO249_003749"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637877.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2845..4338)
                     /locus_tag="OO249_003750"
     CDS             complement(2845..4338)
                     /locus_tag="OO249_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCR5637878.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(4382..4771)
                     /locus_tag="OO249_003751"
     CDS             complement(4382..4771)
                     /locus_tag="OO249_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637879.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4989..5429
                     /locus_tag="OO249_003752"
     CDS             4989..5429
                     /locus_tag="OO249_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5637880.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1432 a         1663 c         1583 g         1358 t
ORIGIN      
        1 aaaaaaaagg aatatttcgt tttcacgagt aatacccggg tattaaaaat ccactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt
      181 ctcttcagta acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaatcc agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag
      301 gttgttagcg ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat
      361 actggcttac tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga
      421 aaaaaggcag caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg
      481 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg
      541 ggacggaaat ttcctggaag ataccaggaa gacacttagc aaagaagaga taaggccacg
      601 gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca
      661 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
      721 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      781 ccgcggtttc tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg
      841 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa
      901 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg
      961 agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga
     1021 aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca
     1081 actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga
     1141 gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc
     1201 ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc
     1261 tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag
     1321 agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa
     1381 acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg
     1441 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1501 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1561 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1621 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1681 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1741 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     1801 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     1861 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     1921 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     1981 cttgatcccg gcatgggttt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2041 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2101 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2161 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2221 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2281 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
     2341 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
     2401 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     2461 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     2521 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     2581 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     2641 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     2701 ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca ctagcggctc
     2761 cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca gcattttgat
     2821 tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct cagccggtgg
     2881 cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct tcaggtgatc
     2941 aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca tggcgccgcc
     3001 gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt gcgcccatgt
     3061 catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga ccttgttgcc
     3121 cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg cgaacacccc
     3181 gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc ttgcaatgaa
     3241 atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct tgagctggta
     3301 gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg ggcggctgat
     3361 gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca cgccggcgtg
     3421 caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac tggtcggcag
     3481 agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac ggtaagtgat
     3541 cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg ccagatagct
     3601 gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca cccggtgggc
     3661 gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc agcggaaacg
     3721 cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt ggaagtgaac
     3781 attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg tcttggccac
     3841 ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt aaacgatgcc
     3901 cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct gaaacgggaa
     3961 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     4021 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     4081 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     4141 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     4201 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     4261 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     4321 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     4381 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
     4441 cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg ttattgcgtg
     4501 caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa ttaatattct
     4561 tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat gcagtataag
     4621 atgaaccact actattatca tggccaccat taccaccacc gttaaaacta cctcccgaac
     4681 ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac
     4741 cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact tttaaaaaca
     4801 gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact acgcccgtca
     4861 aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca gcctcagacc
     4921 ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca ggagacactg
     4981 tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga aagtcgcgct
     5041 cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc
     5101 gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc acttctgaga
     5161 caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg gaaaatgctc
     5221 agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc ctaatgcttg
     5281 aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac
     5341 aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg ggattctggt
     5401 cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt accataacat
     5461 ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac cggtaaaaca
     5521 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5581 gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg taagtcctgt
     5641 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5701 aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta
     5761 caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa aagtgatgta
     5821 taagtgatgt atgaactgaa ggagcgatgt aattctgtag tcaggaagaa taacccgttc
     5881 atgcaggcat gaacgataca gtgaggaacg ctttttttgt cacgatctcc ccttaacctg
     5941 cgtaaatcac ccgccatggt tgattatttt tcaacctcaa aatggtcata cacctaacgc
     6001 agccgcgatt ttcatcgcaa ctgcatgaat taaaag
//
LOCUS       DAJTOR010000209         5969 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTOR010000209 DAJTOR010000000
VERSION     DAJTOR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.209"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..144
                     /locus_tag="OO249_003753"
     CDS             <1..144
                     /locus_tag="OO249_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5637881.1"
                     /translation="CRNDSLMIGRESFSVDRITPDDGGCSYIRLRREGAGNVRAGGYY
                     EGA"
     gene            131..718
                     /locus_tag="OO249_003754"
     CDS             131..718
                     /locus_tag="OO249_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5637882.1"
                     /translation="MRGLENAIRNLNSLDTRMVPQASAWAVNRVAAKVVSVATRQVAR
                     NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLPAIKLGTTCRRAGDG
                     LKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRKNRLPVEVVKIPLAG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            715..1116
                     /locus_tag="OO249_003755"
     CDS             715..1116
                     /locus_tag="OO249_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5637883.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1127..1867
                     /locus_tag="OO249_003756"
     CDS             1127..1867
                     /locus_tag="OO249_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5637884.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTLATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1926..2312
                     /locus_tag="OO249_003757"
                     /pseudo
     CDS             1926..2312
                     /locus_tag="OO249_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
     gene            2321..2649
                     /locus_tag="OO249_003758"
                     /pseudo
     CDS             2321..2649
                     /locus_tag="OO249_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            2621..5662
                     /locus_tag="OO249_003759"
     CDS             2621..5662
                     /locus_tag="OO249_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5637885.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAAGDAERSSARMQ
                     RFMERQTQAARQTMHAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEEFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAVGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIGINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5662..>5969
                     /locus_tag="OO249_003760"
     CDS             5662..>5969
                     /locus_tag="OO249_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5637886.1"
                     /translation="METFRWKVRPDMNVVSEPKVVAVKLGDGYEQRRPAGLNNLLSTY
                     SVTIRVRKGEHPPLKAFLERHGGVRAFQWTPPYDWKPIRVACRKWSASVGALWVTVTA
                     "
BASE COUNT         1447 a         1367 c         1939 g         1216 t
ORIGIN      
        1 tgtcgcaatg attcgctgat gattggccgg gagtctttca gcgtggatcg tattactcct
       61 gatgacggcg gatgtagtta tatccggttg cggcgtgaag gagcaggaaa cgtaagggca
      121 gggggatatt atgagggggc ttgagaatgc catccgcaat ctgaacagtc ttgatacccg
      181 tatggtgccg caggccagcg catgggcagt aaaccgggtt gctgcaaaag ttgtctcggt
      241 tgccacccgg caggtcgcca ggaataccgt tgcgggagat aaccaggtga aggggggccc
      301 cctgaaactg gtgcgtcagc gcgtccgggt atttaaagcc agtccggcag gaaaaatgta
      361 tgcccgtatc cgtgttaacc ggggcaatct gcccgccatt aagcttggca caacatgccg
      421 gcgtgccggt gacggactga aggtggggaa atactttttt cgtggtgcgt ttatccagca
      481 gctggcgaac ggtcgctggc atgttctcag gcgtcttcct gaagcgtgtt ttgaaacagg
      541 aaaggacagt aaggggcggc ccagaaaaaa tcgtctgccc gtggaggttg tgaaaattcc
      601 gctggccgga ccgctgacac aggcatttga ggatgcccgc gaccgcatca ttgccgcgga
      661 aatgccgaaa cagctggggt atgccctgaa acagcaactg aggatacatt tgggatgaac
      721 aggcacacac aaatccgcca ggcagtactg gcaagcctga agggcgaatg cggggggaat
      781 gtcgtgcttt ttgacgggct tccggcattt attgatgcgc aggaactgcc cgccgtggcg
      841 gtgtggctga gtgatgcgca gtacaccgga aaaatgacgg atgaggacga ctggcaggct
      901 gttctgcata ttgccgtttt cgtccgtgca caggcgccgg attctgagct ggatacgtgg
      961 atggagaaca ccatatttcc ggccatgaag gatattccgg tgctttccgg actcatcgac
     1021 accatgatcc cactcggttt taactatcaa cgtgataatg agatggccac ctgggcgatg
     1081 gcggaaatca cgtaccagat cacgtacacg aattaaggag gtggcaatga ccacaccaaa
     1141 tccactggca aagacgaaag gtgcgggaac gacgttctgg atgtacaccg gcaagggcga
     1201 tgcgtttgcg aaccctttgt cggacactga ctggctgcgt cttgcgatgg tgaaggatct
     1261 gcaacctggc gaaatgaccg ctgatgcaga agatgacact tatctcgatg atgaagatgc
     1321 agactggaaa acgacaaccc aggggcagaa atccgtcggt gatactttgg cgacgctggc
     1381 ctggcgtccg ggtgacagcg ggcagaaaaa actggttcag ttgttcgatt ccggtgaagt
     1441 ctgcgcgttt cgtatcaaat accccaacgg tacggttgat gttttccgtg gctggctgag
     1501 ttcactgggt aaaaccatta cctcaaaaga ggtgatgacc cgtactgtaa aaatcagcgg
     1561 tgtggggcgt ccgtatctgg cagaggaggg cgctgaaacc gtgggtgtta ccggactgac
     1621 ggtggcaccg gcatctgtca gtgtcaaagc gggagcaacc accacactga cctttacagt
     1681 aaaacctgac ggggccagtg acaaagcgat cagtgtgcat tcgtcagatc ctcagactgc
     1741 ttcggtgacc ctgagcgggc ttgtggccac ggtgaaaggc gtgaagcagg gcagtgtcag
     1801 catcgtgggc atgacctctg acggagagtt tgtggcagtg gctgcggtga ccgtcagcgc
     1861 accataacag gacgatactc atcattgccc cggttatccg gggctttttg catccggaga
     1921 acatgatgtt tctgaaacag gatacgttta attatgaaaa acagtccgtg gtgctcagtg
     1981 agctgtccgg gctgcagaga attgaatatc tgacgtttgt tcagcagcga acggcaaagt
     2041 ttgatgcaca ggagggagaa ctgccggagg ctgaacgaca gattgctttt ctgcgtatgg
     2101 gaatggatat caatgcctgg ctggtttccc gctcactgtg gaatgctgag cagtctcagg
     2161 atgttgagac gctttgagca tccattatga caacatggtc gtatgatgcg ctgggcgcgg
     2221 gggcggagag ggttctgtcg ctgagcggta tggggaccat tgagaatgcc ggggatgatg
     2281 atcatgaggc gctgacgccg gaaaagtcct gacgcgggaa atgcagtttg tcatgcggct
     2341 tgcccgggag ttccggcggg cagactggcg gcggatgctg tcggaaatgt cggccactga
     2401 gcttggtgag ggggcgatta tttccggatg cagagcttca gtgatgtgtg gatggatgcg
     2461 cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggcagcag tgatgctgcg
     2521 gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac ggacgaagaa
     2581 ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga tagccaacct
     2641 ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc gtatcaaaaa
     2701 ccttctgaat ggtgcagccg gtgatgcaga acggtcttct gcccgtatgc agcgttttat
     2761 ggagcgtcag actcaggcgg cccggcagac aatgcatgcg gcttcttcgg ctgcaacagc
     2821 agcatcagcc catgcgcaga cggtggagaa gaatgcacgg gctcatgaac gcatggcccg
     2881 tgaggtggaa caaacccgtc tgcgcgtgga tgccctgaat cagaaaatgc gcgaggaaca
     2941 ggcgcaggcc agggcactgg cggaggcgca ggataaagcg gctgccgcct tttatcgcca
     3001 gattgacagt gtgaaacagg ccggtgcggg gcttcaggaa ttacagcgta ttcagcagca
     3061 gatccgacag gccagaaaca gtggcggggt tggtcagcag gattatctgg cgctgatttc
     3121 ggagatcacg gcgaaaaccc gtgccctgac gcaggcagag gaacaggcca cccggcagaa
     3181 agcggcgttt atccgccagc ttaaagagca ggcaacccgc cagaatctgt cgtcttctga
     3241 gttgcttcgc gccagggcgg ctcagctggg ggtaagcagt gctgcagaag tgtatatccg
     3301 caaaatggag cgggcaggaa aagccacaca ttcgctgggg ctgaaaagtg cggcagcccg
     3361 gcgggagctg ggggtgttaa tcagtcagat ggcgcgcggc aattttggtg cgctgagggg
     3421 atccgggata acgctggcta accgtgccgg atggacaggc gcactgatgt cgccgaaagg
     3481 catgatgact ggcggcgtta ttggcggact tgtcgcggcg gtcctgggtc tgggtaaagc
     3541 ctggcatgac ggccggaagg agggcgagga atttaaccgt cagctggcgc tgacgggaca
     3601 ttatgccggt gtcactgtcg ggcagttgtg gaaactcagc cgggccatat ccgggaatgg
     3661 tatcacgcaa catgcggcag ccggtgcgct ggcacaggtg gtgggcagtg gggcatttca
     3721 tggaaacgat attggtatgg tggcgaaagc tgccgcacag atggagcgat cggttggcca
     3781 gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg taaatgccgc
     3841 gaaggcgctg gacaatgcgc tgcatttttt gactgccact cagcttgagc agatacgcgt
     3901 ccttggggag caggggcggt ccagtgatgc ggcccggatc gccatgtctg cgctggcaga
     3961 ggaaaccggt aaacgcacat ctgatattga taataatctc aatgcgctgg gtagtacgct
     4021 gcaaaccttg tctgactggt ggaagcagtt ctgggatgcg gccatgaata ttggtcgtga
     4081 agactcgctg gatgcgcaga ttgatgcgtt acaggaaaaa attcagcgcg cgaaaaaata
     4141 tccgtggaca aacgcctcca cacaggtgga gtacgatcag cagcgtctta acgatcttca
     4201 ggagaaaaaa cgccggaagg atttgcagga tgcaaaagcg caggcagaac ggaattacca
     4261 ggagcaacag aaacgccgga atgctgaaaa tgccgcgctg aaccggatga atgaaacgga
     4321 agctgcacga catcagcggg aaattgcgcg tattaatgcc atgcagtacg ccgaccaggc
     4381 tgtcagggat gcggcgatac agcgtgaaaa tgaacgttac gaaaaagcca ttaagaaaaa
     4441 tacacgggca acccgtaatg atgaggccac ccggttattg ctgcagtaca gtcagcagca
     4501 ggcacaggtg gaagggcaga ttgctgctgc cagacagtca gcaggcattg ccactgaaag
     4561 gatgactgaa gcgcataaac agcttctggc cctgcagcag cgcatcagcg acctggacgg
     4621 gaaaaagctg acggcagatg aaaagagtgt gctggcccgt aagaatgagc tgattcaggc
     4681 gctgacgctg ctggatgtga aacagcagga gctgcagaaa cagacagcgc ttaacgacct
     4741 gagaaaaaaa acggttcagc tgaccagcca gctggcagac aaagaacgtg cactgcgtga
     4801 gcagcacaat ctggatattg ccactgcagg tatgggggat aagcagcggc agcgctacca
     4861 ggcacagttg cgcatccggc aggaataccg gcaacagttg caacagcttg agaatgacag
     4921 tcgccagaaa ggcacttacg ggacggagga ctaccggagg gctgaggagg tgctgaaggg
     4981 gagcctgaag cgacaactga atgaaaacaa acgctactgg caggaactgg aagtggcgca
     5041 gggcgactgg aaaaacggtg ccatgcgggc gtttcagaat tttacggcgg atgcggataa
     5101 tgcggtggga acggcagaac agatgtttac agtggcattc agcagtgccg gtaatgcact
     5161 ggcgacattc tgtaccaccg gtaagctgaa ttttaaatcc ttcacctctt ccctgttgtc
     5221 agatatggcc agaattatgg cacagatggc catgatgcag gcggtaaagg gcgtcggctc
     5281 tttattcggc ttcacgacta atgctgatgg cggtgtttac cagtctgctg atttgagtcg
     5341 ctacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag gcgcgggtgt
     5401 gatgggggaa gcgggacctg aagccattct gccactgcgt cgcggtgctg atggtaagct
     5461 gggggttgtg gcggatactg gtggttcagg catggtgatg tttgccccgc agtacaacat
     5521 cgggatcaat aacgacggca cgaacgggca gataggtccg gcagcactga aggtggttta
     5581 tgacctcggg aaaaaagcgg ccgcggactt tatgcaacag caggcccgtg atggtggccg
     5641 gttaagtgga gcataccggt aatggagacg tttcgctgga aggtgcgccc ggatatgaat
     5701 gtggtatcag agccaaaagt ggtggcagtg aagctgggtg atggttatga acagcgtcgc
     5761 ccggcgggac tgaataatct gctgtcgact tacagcgtga cgatacgtgt tcgtaaaggt
     5821 gaacacccac ctttaaaagc ctttctggaa cggcacggtg gcgtccgcgc atttcagtgg
     5881 acgccacctt atgactggaa accgatcagg gtggcttgtc gtaaatggtc ggcaagcgtg
     5941 ggggcgttat gggtgactgt aacggccga
//
LOCUS       DAJTOR010000210         5802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTOR010000210 DAJTOR010000000
VERSION     DAJTOR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.210"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(100..2202)
                     /locus_tag="OO249_003761"
     CDS             complement(100..2202)
                     /locus_tag="OO249_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5637887.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPSDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAVGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2444..3505
                     /locus_tag="OO249_003762"
     CDS             2444..3505
                     /locus_tag="OO249_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR5637888.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3618..5117)
                     /gene="ansP"
                     /locus_tag="OO249_003763"
     CDS             complement(3618..5117)
                     /gene="ansP"
                     /locus_tag="OO249_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR5637889.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5384..>5802
                     /locus_tag="OO249_003764"
     CDS             5384..>5802
                     /locus_tag="OO249_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HCR5637890.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLTLENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWL"
BASE COUNT         1540 a         1495 c         1381 g         1386 t
ORIGIN      
        1 acattgtctt gctgaaatta tgcaacaaaa tattgctatt ttataccagc aatacaatcg
       61 ttaaagtaaa tgcccggcaa ataccgggca tcaaggtaat tactcaaatc tccacgcaat
      121 attcatgccg acaccataat ttcgtccggg cgaaggttcg taatatcgcc cgtttgactc
      181 attgacaatg acagaaccaa cgtattcttt atcgaataaa ttatcgacac gaccaaataa
      241 atccacagtt aaattgtggt aattgtattt atacccggtg aataagccga cgagagtata
      301 agacggcgct tttgccgtat tttcatcatc tgccataata tcgcccatat aacgcgcttc
      361 tgtgcctgca taccaaccat cttccggtac ataacctatc gacgcaaagc ccatattacg
      421 ggcgatccct ggcatccgat taccgttaca atcctgttca ttgcaaacat tgctgcgata
      481 ggtcgcatca agccaggtcc aggacgcgtt tacgcgaaaa tctcctgcga aacgttgatc
      541 ccatgccagt tcagcgcctt gacgacgggt ctttccggca tttttgtaag tcgtacgccc
      601 accgctactg ctatcgacaa caatttcatc atcagtgtcg gtctgaaaca atgcgagact
      661 aagcagccca tcaccaatac gcgttttact gccgatctca attgtatcgt tggtggatgg
      721 ttttaaacca aagttcatac cgctttgccc atcagcacga taagacagct cattaatcgt
      781 cggcgtttca aaacctcgcc cgactgccag atagatattc caggcatcgg tcattgcata
      841 ttttaacgaa ccggcaggta gccatttatg ataactggca tcaccgctgt catcgccgtt
      901 acccggagta acgtaatggt cgttggaatc aaaccacacg gagctgtagc gcacgccagc
      961 atccagcgac agtttttcgc tcagctgcca ctgcgtctgt aaatagggat cgatgttcca
     1021 catcagattg cgttcgtcgc gacgcaactc acctttttgc ccgtactccg gcatgccgct
     1081 attcaggcgg aagttattgt agcccttgcg gttttcactc atgttttcgt agttcaggcc
     1141 ggtagtgaac gtgaccggaa cgcccagttc accacggtgt gtccagcggc tgtctatccc
     1201 ctggtaatgg cgttgcaggg taatcacgcc gcccgcatgt gacgggttaa gttgtggtgc
     1261 catgggtatt gactggtact gggtcgtttc tcgctctccg gcatacatca tcacactcat
     1321 atcatcttgc gcgctcaggc tacgctcata gcgcaaccct gcctgagttt gcttgatggt
     1381 ttttcgcgtg tcgtactgtt ctgcacgagg cgcttgttgt ggattagcct tccattctgc
     1441 tttggttagc ccacctgggt catctgcttt gatatccaca ctattgaaaa tcagacttaa
     1501 tttgctggct tcatcaatgc gtacgcccag tttggcattg gctaaatttt tctgtgcgcc
     1561 actatggtca cgatagccgt gggtcgtaaa acgcgtggtt gagacggtgt aatcgacatc
     1621 gctaggctgt gtgccgtctc ccgttgcgcc cgttgctttc agcccatagc gccagctgcc
     1681 aaaactgccg tagtaactac tggcttcaat ggttggtggc tgttgtccgg tctgggtggt
     1741 gacattcatt accccaccag acgcgttgcc atacagggca gagaaggggc cacgcagcac
     1801 ttccacattt tgcacactgc ttaaatcgat gttggatgtt tgcccttgcc cgtcgggcat
     1861 ggtggcggga ataccgtcca catacaggcg aataccgcga ataccgtaag tggagcggga
     1921 gccaaatccg cgaatcgaca gctgtaaatc ttgcgcatag ttctgccggt tttgtacctg
     1981 caaaccaggc acgccggtca gtgattcgga caagttaatg cgcggtgttg ccaggcgcat
     2041 ctcctcgcca tccaccacgc ttactgctgc tggggtatcc agttctgaaa ccacctgcgg
     2101 tgcggcactg acaatcatag tctgttcatc agcggcaaaa acaacgggag aaaggacaag
     2161 cagtgcgggc aaaacggtct gtcggacgga aaaaatcttc atgaaaaaag ccaggttaag
     2221 aatgggaaaa cgctgtcatg gtaataaaat tgtaaactta tgcaaaacga aacggcacga
     2281 tacgttaatt aattgagaaa attgtagtcg taacggcaag aaatgctcca catttgagaa
     2341 aataatgatt accattccca tttataacaa gagcgtgacg atgattacgc ttagcgaagc
     2401 attgtgaagc agcaaaaata ttggttcatc aaagggagtc gtcatgcatt tacgtcatct
     2461 gttttcatcg cgcctgcgtg gttcattact gttaggttca ttgcttgttg cttcatcatt
     2521 cagtacgcag gccgcagaag aaatgctgcg taaagcggta ggtaaaggtg cctacgaaat
     2581 ggcttatagc cagcaagaaa acgcgctgtg gctcgccact tcgcaaagcc gcaaactgga
     2641 taaaggtggc gtggtttatc gtcttgatcc ggtcactctg gaagtgacgc aggcgatcca
     2701 taacgatctc aagccgtttg gtgccaccat caataacacg actcagacgt tgtggtttgg
     2761 taacaccgta aacagcgcgg tcacggcgat agatgccaaa acgggcgaag tgaaaggccg
     2821 tctggtgctg gatgatcgta agcgcacgga agaggtgcgc ccgctgcaac cgcgtgagct
     2881 ggtagctgat gatgccacga acaccgttta catcagtggt attggtaaag agagcgtgat
     2941 ttgggtcgtt gatggcgaga atatcaaact gaaaaccgcc atccagaaca ccggtaaaat
     3001 gagtaccggt ctggcgcttg atagcaaagg caaacgtctt tacaccacta acgctgacgg
     3061 cgaattgatt accatcgaca ccgccgacaa taaaatcctc agccgtaaaa agctgctgga
     3121 tgacggcaaa gagcacttct ttatcaacat cagccttgat accgccaggc agcgtgcatt
     3181 tatcaccgat tctaaagcgg cagaagtgtt agtggtcgat acccgtaatg gcaatattct
     3241 ggcgaaggtt gcggcaccgg aatcactggc tgtgctgttt aaccccgcgc gtaatgaagc
     3301 ctacgtaacg catcgtcagg caggtaaagt cagtgtgatt gacgcgaaaa gctataaagt
     3361 ggtgaaaacg ttcgatacgc cgactcatcc aaacagcctg gcgctgtctg ccgatggcaa
     3421 aacgctgtat gtcagtgtga aacaaaaatc cactaaacag caggaagcta cccagccaga
     3481 cgatgtgatt cgtattgcgc tgtaatagat atggcgaggg tgaaaacggt gagacttgcc
     3541 tgccttttat cacggcgtat ccggcacttt cgtcagtaat ctgaatttgc ccggcacgta
     3601 gccgggcaat tttgcaatta actcgccgtt tcaggcttaa acacaatttc ctgtttttct
     3661 tcatcttcct cgacgactgg cgcagtgctg tgaatttcag caacgcgttt gcgcacacca
     3721 aaccagccta taaccagcag aataccgata atcggcagcg ccgcgatagt ataagtcccg
     3781 ttcgggtaat cgaacgccat cagcacaagg acactcagta aaaacagtaa tgtcagccag
     3841 gaagtgaagg gcgcgccagg cagtttaaaa ctgacatccg ctgctttgcc ttctttaatc
     3901 gctttacgca ggcgcatctg gcacacgatg ataaacgccc atgaagcgat gattcccagc
     3961 gacgcgaagt tcaacacaat ctcaaatacg cgcgacggca ccagatagtt gaggaatacg
     4021 ccgacgacat acacaaccag tgtcgctaga atcccggcat acggcacatg ctgacgactc
     4081 attttcgcca taaaactcgg tgcggaaccg cccatcgcca ttgagcgcag aatacgtccg
     4141 gtgcagtaca gacctgagtt caggctggag agggcagcgg tcagcaccac aatgttcata
     4201 atgctgccga tatatggcac acccagttta gagaaaaacg tcacgaacgg actttgcccc
     4261 gcctgatacg cgctccacgg caataacata accagcaaca ccacggagcc gacgtaaaac
     4321 aggccaatac gccaaatcac actgttaatg gctttaggca ccatggtctg cggatcttta
     4381 cattctccgg cagctgtacc caccatttca atggaggcaa aagcaaacac tacgccctga
     4441 atcaacacca gcgcaggcag caaaccgtgg gggaagaagc cgccattatc ggtgattaaa
     4501 tgaaagccag tggtgttgcc atccagcggc tgaccactac cgaggaacac tgtacccacg
     4561 accaaaaagg tcacaatggc gagcacttta ataagcgcaa accagaactc catctccgca
     4621 aaccatttca caccgatcat attcatggtg ccaacgatgg taagtgcagc gagcgcaaag
     4681 acccactgcg gcacgccgcc aaacgcaccc cagtaatgca tatacagagc gacggcggta
     4741 atatcaacaa tccccgtcat cgcccagttg atgaagtaca tccagccagc aacataagcg
     4801 gctttctcac ccaaaaactc acgggcataa gagacaaaac tgccactgga agggcggtgt
     4861 agcaccagct cacccaatgc acgcagaata aaaaacgaaa acaagccaca aattaaataa
     4921 accagtgcca gtgctggacc cgccatttgc agtcgggctc ctgcacctaa aaacaagccg
     4981 gtgccaatcg cgccgccaat ggcgatcatc tgtacctggc gattgcccat cgctttgtga
     5041 tacccctctt cgtgggcatt aagccagcgg cgtttcgcgg cgtgttgatc tgaagtgtcg
     5101 gtgtcgtgtt tactcattgc tctccctgat tgctttaatg aaaaagtcat ataagttgcc
     5161 atgaacaatg tttattcatc ctggcgatga tagttatcac cattattctt tatgggtata
     5221 aaactatcgg cgcagcatcc tacccgttat cgatatacga tgcaaaacat ccccttacaa
     5281 tcctgaaggg gattcataca actgacgaaa aaatgacaaa tacttttgct ggttaacctg
     5341 tgtactgtcc tacacttaat ctttaaaaga ttgtgagggg catatgatta aggtgtatgg
     5401 cgtacccgga tggggctcta caatcagtga gctgatgcta acactggctg atattcctta
     5461 tcagttcgtt gatgtcagtg gttttgacca cgagggaact tcacgcgagt tattgaaaac
     5521 cctgaacccg ttgtgtcaga ttccgacact gacgctggaa aatgacgaga ttatgacgga
     5581 gacagcggcg attgcattga tggttctcga tcgtcgcccg gaccttgcgc caccggttgg
     5641 gcgcgccgaa cgtcagcagt ttcaacggct attggtctgg ctggttgcta atgtctatcc
     5701 aacattcact ttcgccgatt accctgagcg ttgggctcct gacgcacctg aacagttaaa
     5761 gaagaatgtt attgaatatc gtaaatcgct ttatatctgg ct
//
LOCUS       DAJTOR010000211         5791 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTOR010000211 DAJTOR010000000
VERSION     DAJTOR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.211"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..831
                     /gene="pdeC"
                     /locus_tag="OO249_003765"
     CDS             <1..831
                     /gene="pdeC"
                     /locus_tag="OO249_003765"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR5637891.1"
                     /translation="GSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQPIIDIKT
                     EKCIGAEALLRWPSEQEQVMNPAEFIPLAEKEGMIEQVTNYVIDNVFRDLGDYLATHA
                     DRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMTPIILAF
                     RQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHIIELAH
                     SLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPARELTRGQ
                     "
     gene            complement(834..1157)
                     /gene="soxS"
                     /locus_tag="OO249_003766"
     CDS             complement(834..1157)
                     /gene="soxS"
                     /locus_tag="OO249_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5637892.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1243..1707
                     /gene="soxR"
                     /locus_tag="OO249_003767"
     CDS             1243..1707
                     /gene="soxR"
                     /locus_tag="OO249_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5637893.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYRRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2253..3602
                     /gene="ghxP"
                     /locus_tag="OO249_003768"
                     /gene_synonym="yjcD"
     CDS             2253..3602
                     /gene="ghxP"
                     /locus_tag="OO249_003768"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5637894.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3754..5403
                     /locus_tag="OO249_003769"
     CDS             3754..5403
                     /locus_tag="OO249_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5637895.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5557..>5791)
                     /locus_tag="OO249_003770"
     CDS             complement(5557..>5791)
                     /locus_tag="OO249_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5637896.1"
                     /translation="MFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1258 a         1370 c         1595 g         1568 t
ORIGIN      
        1 gggagtcttg ttctgctatt actctggcta cgtattcgac aaaactattt gtctcccaaa
       61 cgcaaattgc aacgcgctct agaaaaacat caactttgtc tttattacca gccaataatc
      121 gatatcaaaa cagaaaaatg tatcggcgct gaagcgttgt tacgttggcc tagtgagcag
      181 gagcaagtaa tgaatccggc agagtttatt ccgctggcag aaaaggaggg gatgatagaa
      241 caggtaacta attatgttat tgataatgtc ttccgcgatc tgggcgatta cctggcaaca
      301 catgcagatc gctatgtttc tattaacctg tcggcctccg attttcatac gtcacggttg
      361 atagcgcgaa tcaatcagaa aacagagcaa tacgcggtgc gtccgcagca aattaaattt
      421 gaagtgactg agcatgcatt tcttgatgtc gaaaaaatga cgccgattat tctggctttc
      481 cgccaggctg gttacgaagt ggcaattgat gattttggta ttggctactc taacttgcat
      541 aaccttaaat cattgaatgt cgatattttg aaaatcgata aatcgtttgt tgaaacgctg
      601 accacccaca aaaccagtca tttgattgcg gaacacatca tcgagctggc gcacagcctg
      661 gggttaaaaa cgatcgctga aggcgtcgaa actgaggagc aggttaactg gctgcgcaaa
      721 cgcggcgtgc gctattgcca gggatggttt tttgcgaagg cgatgccgcc gcaggtgttt
      781 atgcaatgga tggagcaatt acccgcgcgg gagttaacgc gcgggcaata aaactacagg
      841 cggtggcgat aatcgctggg agtgcgatca aactgccgac ggaaaacgcg ggagaaggtc
      901 tgctgcgaga cataacccag gtccattgcg atatcaaaaa tcggacgctc ggtggtgcgc
      961 aactcaacgg cggccagtaa caggcgacgt tggcgaatgt aatcgccaag cgtctgatgc
     1021 gtcaccgtgc ggaacattcg ttgcaagtac cactttgaat agcctgattt ttttgcgact
     1081 acatcaatgt taagcggctg gtcaatatgc tcgtcaatcc atgcgataag atcctgaata
     1141 attttctgat gggacataaa tctgcctctt ttcagtgttc agttcgttaa ttcatctgtt
     1201 ggggagtata attcctcaag ttaacttgag gtaaagcgat ttatggaaaa gaaattaccc
     1261 cgcattaaag cgctgctaac ccccggcgaa gtggcgaaac gcagcggtgt ggcggtatcg
     1321 gcgctgcatt tctatgaaag taaagggttg attaccagta tccgtaacag cggcaatcag
     1381 cggcgatata gacgtgatgt gttgcgatat gttgcaatta tcaaaattgc tcagcgtatt
     1441 ggcattccgc tggcgaccat tggtgaagcg tttggcgtgt tgccagaagg gcatacgtta
     1501 agtgcgaaag agtggaaaca gctttcgtcc caatggcgag aagagttgga tcggcgcatt
     1561 cataccttag tggcgctgcg tgacgaactg gacggatgta ttggttgtgg ctgcctttcg
     1621 cgcagtgatt gcccgttgcg taacccgggc gaccgcttag gagaagaagg taccggcgca
     1681 cgcttgctgg aagatgaaca aaactaaagc gccacaaggg cgctttagtt tgttttccgg
     1741 tctttgtctt tctctctatc ccgctggtac acaggagggt ttcccccgac gtcaacacac
     1801 ctcattcgag cacgtggtgg aggttccggt tggtgttgat gctttaattg tatgtcaccg
     1861 acgtttcttc gccagtgtaa aagtatactt tttaaccgca atatttttgt catctcagac
     1921 gattttttat cgcaatcctg aacggtatac ggctcgataa cgctgcaatc ttgcgcaccg
     1981 acgataacgt ttgcgcatca attgcctgtt ttttcatcgt caagacaata aaagagaaaa
     2041 aagcagcaaa cttcggttga aaaagccgct atgatcgccg gataatcgtt tgcttttttt
     2101 accacccgtt ttgtatgcgc ggagctaaac gtttgctttt ttgcgacgca gtaaattgtc
     2161 gcaaacctgg agcaggaaga taacgtttcg ctggcagggg attgtccgcc acgcatcttg
     2221 acgaaaatta aactctcagg ggatgttttc ttatgtctac gccatcagcg cgtaccggcg
     2281 gttcactcga cgcctggttt aaaatttcac aacgtggaag cactgtccgt caggaagtgg
     2341 ttgccgggtt aacaacgttt ctggcgatgg tctactcggt catcgtcgtt ccaggtatgt
     2401 tgggtaaagc aggcttcccg cctgcggcag ttttcgttgc aacctgtctg gttgccggac
     2461 tcggttctat cgtgatgggt ctgtgggcta atctgccgtt ggcgattggt tgcgccatct
     2521 ccctgacggc gtttaccgca ttcagcctgg tgctggggca acatattagc gtacctgtcg
     2581 cgctgggtgc cgtgttcctg atgggtgtgc tgtttacggt aatttctgcc acgggtatcc
     2641 gtagctggat tttgcgcaac ttgccgcacg gtgtggcgca cggcacgggg atcggtatcg
     2701 gtctgttcct gctgctcatt gccgctaatg gtgttggtct ggtgattaaa aacccgcttg
     2761 atggtctgcc cgttgcgctg ggtgatttcg cgaccttccc ggtgattatg tcactggtag
     2821 gtctggcggt gatcatcggc ctggaaaaac tgaaagtccc tggtggcatt ctgctgacca
     2881 ttatcggtat ctcaattgtc ggtttgatct tcgatcctaa cgtccatttc tccggcgttt
     2941 tcggcatgcc ttcattgagc gatgaaaacg gcaattcact gattggcagc ctggacatta
     3001 tgggcgcgct gaatcctgta gtcctgccaa gcgttctggc gctggtgatg acggcagtat
     3061 ttgatgccac cggaactatc cgtgccgtcg ccggccaggc gaacctgctg gataaagatg
     3121 ggcagatcat cgacggtggg aaagcactga ccactgactc catgagcagc gttttctctg
     3181 gcctggtggg tgctgctccg gcagcggtat acatcgagtc tgcggcgggt acggcggcgg
     3241 gcggtaaaac cggtttgacg gctatcaccg ttggcgtgct gttcctgctg attctgttcc
     3301 tctctccgct ctcttacctc gttccggggt atgcaacggc tccggcgctg atgtacgttg
     3361 gcctgctgat gctgagcaac gtggcgaaaa tcgactttgc tgattttgtt gatgcgatgg
     3421 cgggtctggt tacggcggta ttcatcgtgc tgacctgtaa catcgtaaca ggcatcatga
     3481 tcggcttcgc gactctggtg attggtcgtc tggtttccgg cgaatggcgc aagttgaaca
     3541 tcggtacggt cgttatcgcc gtggcgctgg tgaccttcta tgcgggtggc tgggctatct
     3601 aatctttcat cggattctga aaacgggtgg caatggctgc ccgtttttat tttctccgca
     3661 cattgtgtga gcttttgcga tattctgaaa aaatgagaat tcaggcataa cgcctttctc
     3721 agatcataaa aaaacatcgc aaacagggaa cgcatggaaa tcttcttcac catactgata
     3781 atgaccctcg tggtctcgct gtccggggtg gtcactcgtg tcatgccctt tcagatcccg
     3841 cttccgctta tgcaaatcgc catcggtgcg ctactggcgt ggccgacgtt tggtttgcat
     3901 gtggagtttg atcctgaact ctttttagtc ttgtttatcc cgccgttgct gttcgctgat
     3961 ggctggaaaa cgccgacccg tgaatttctc gaacatggtc gagagatttt cggcctcgcg
     4021 ctggcgctgg tggtggtcac cgtggtcggc attggcttcc ttatttactg ggtggtgccg
     4081 ggcattccgc tgatccccgc ctttgcgctg gcggcggtac tttctccgac cgatgctgtg
     4141 gcgctctccg gaattgttgg cgaagggcgc atcccgaaaa aaatcatggg cattttgcag
     4201 ggcgaagcgt tgatgaatga cgcctccggc ctggtgtcgt tgaagtttgc cgtggcagtg
     4261 gcgatgggga cgatgatctt caccgtcggc ggtgcgacgg tcgaatttat gaaagtagcc
     4321 attggtggta ttcttgccgg ttttgtggtg agctggctgt atggtcgctc gctgcgattc
     4381 ctcagccgct ggggcggtga tgaacccgcg acgcagatcg ttctgctgtt cttgctgcca
     4441 ttcgcttctt atctgattgc cgaacatatt ggcgtttccg gcatcctcgc tgcggttgcc
     4501 gccgggatga ccatcacccg ctccggtgtg atgcgccgtg cgccgctggc aatgcgcctg
     4561 cgcgcaaaca gcacctgggc gatgctggaa tttgtcttta acggcatggt gttcctgctg
     4621 ttaggtctgc agctgccggg tattctggag acgtcgctga tggcggcaga aatcgaccct
     4681 aacgtcgaaa tctggatgct gtttaccgat attattctga tatatgcggc gctgatgctg
     4741 gtccgtttcg gctggctgtg gacgatgaaa aagttcagca accgcttcct gaagaagaag
     4801 ccgatggagt ttggttcgtg gaccacacga gaaatcctga tcgcgtcttt cgccggggtg
     4861 cgtggggcga tcactctggc cggtgtgctc tctatcccgc tgctcttgcc ggatggtaac
     4921 gtcttcccgg cgcgctatga gctggtgttc ctggcggctg gcgtcattct cttctcgctg
     4981 tttgtcggtg tggtgatgtt gcctattctg ctacaacaca ttgaagtcgc ggatcattcg
     5041 caacaattga aagaggaacg tattgcgcga gcggcaacgg cagaagtggc gattgtggcg
     5101 atccagaaaa tggaggagcg tctggcggcg gataccgaag agaatatcga taaccagctg
     5161 cttacggagg tcagttctcg cgtcattggt aacctgcgtc gtcgcgccga tggacgtaac
     5221 gacgttgaaa gttccgtgca ggaagagaac cttgagcgtc gcttccgtct ggcggcattg
     5281 cgttctgaac gtgctgaact gtaccacctg cgcgccacgc gggagatcag caacgaaacg
     5341 ctgcaaaaat tactgcacga tctcgatttg cttgaagcgt tgctaattga ggaaaatcag
     5401 taagccgaaa gcgacgtaac caggcccgag gtaatctgaa aaaggtgcaa tcttttgcac
     5461 ctttaacagg ttagctcggg ctaacattca tgattctaaa acaaataagt tatctctctt
     5521 gtaaagtcaa ttcattggct gttcttcgac ttaacctcaa tctgcgtgtt gcataatatc
     5581 tgcagtaatc ttcatagcga ctgcattaat attatcctca gtttgtgaca tgccagtatg
     5641 agaggtacaa tcttcttcaa agaatttctg tagtgttacc agggtatcac tatcaatggg
     5701 gatattttcc tctccacgga gtatggaaat gcccattccc tggacgctag cctcatcaaa
     5761 attaacccga tagagacggg tattaaacat a
//
LOCUS       DAJTOR010000212         5744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTOR010000212 DAJTOR010000000
VERSION     DAJTOR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.212"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..382)
                     /locus_tag="OO249_003771"
                     /pseudo
     CDS             complement(1..382)
                     /locus_tag="OO249_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(568..3876)
                     /gene="icsA"
                     /locus_tag="OO249_003772"
                     /gene_synonym="virG"
     CDS             complement(568..3876)
                     /gene="icsA"
                     /locus_tag="OO249_003772"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5637897.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISHSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4405..5607
                     /gene="virA"
                     /locus_tag="OO249_003773"
     CDS             4405..5607
                     /gene="virA"
                     /locus_tag="OO249_003773"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5637898.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQLNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1777 a         1298 c          949 g         1720 t
ORIGIN      
        1 gcttccccat gtgatttgcc tcgccacttc accgggctca ggaccccgta ggatcttcac
       61 cgaaaaaaat gcatcaccag ttgctttatt atttgtttta ggtagacctg ttactaattt
      121 atacctactc tgaccaagtc tggataagaa aggcacacct gcacatttct acttggaaat
      181 gttttctttt accattttgc tcaaaaattt caacatccaa tattcctcgt acagaactac
      241 tcaagtcctg aatagaaaaa gttccagctg gtacagtaga gtcatatatt gctcgacctt
      301 gttgagatac aacaacacgt gcattagttt ctgcaatacc agtaatctga ggcgcataac
      361 cacgaatatt tggcggtagc attcgttcat cgctttctaa tgcaattcct gtgtaacgcc
      421 aagcaacagc gcattctggc gcatgatctg gtaaacacgt ttggcattga tcgcaggcat
      481 accatcatgt gcacaaaaac ccaccgagct tttacacggt gggtcccaga gaaatgcagg
      541 acatcaacac gccctgcatt tttattatca gaaggtatat ttcacaccca aaataccttg
      601 ggtgtctctg taactgttat tacccaattg ctgagatacg tttccccaga tgcttaggtt
      661 atctgcaaga cgccccccca caccagtttt gagttcaatc acattacggt tgcctatctg
      721 gtgatttgac atgccattca taattacacc atattgcttt gagctccatt tccaattcac
      781 ttccaaaaat ggttcgaact tacgaagagt ctccttatcg atacaactat gactaattaa
      841 ggatgtacgc aatcccaatt ttgcttgaat attattgacc ccagaggatg tgaccatagt
      901 gccattgtgt tcaatatagt cgtttgcttt taccccatgc ctagttatct ggacttgtgg
      961 attgagatac aatgcgttgt caatattatt atgagtagtc cagcgtatgg ttgggtagat
     1021 atagccagct tccaaagctc ctgttattcc tttgctggaa tatttctcac cagttaaccc
     1081 atctcctttg actgtattat taaaccaacc gtactgcaac caagcatcca tatatagacc
     1141 tgttcgttct ttttcatcct gaaaccaaga actgtacaaa ccggcagtat aaccgctcac
     1201 agttcctcgt gaactataac tagtatactt attatgagtt ttgctatcct gatatccata
     1261 agctcccata atacccacat gcaagccatc atgatcagtg aagtttgtct tcaaaatatc
     1321 tccaccaatc tgataaacca tcctgttagc tgtggttttt aattgtctat cagctaaatt
     1381 atgctcatta tgtccaccaa cagtacgaat ccacatggtg gtttcgctga gctgacctgt
     1441 agtaggatca acaatagtgc taccgtgtcg gtcatagaga gaaggactaa acatcgtgtt
     1501 cattgctatc aggttagcaa tataactgcc agcttcaggg cgccatgtgt gaatactacc
     1561 ttcacccaaa ttaagtgaag atacaagctg tgaactagct cggcgactac tcatttgagt
     1621 agactcttga ttgctcatct gttttgattc ttgattatcc atctgactag ttagatacca
     1681 cttattggta ttcagtccag atacagtgcc ctgtttcagg cgatagtcat aactaccagc
     1741 aacaatacgg cctttctgaa taaaagcatt cttatcagaa gaatctgtcg aaataatttg
     1801 aacaccttca agagttttat ctccagagcc tccctcattt gtcactgcaa cataggtaat
     1861 accagacgca tggcctgtaa tttccagccg gtcagtttta ctgttgtcat tgccgagaac
     1921 cgcgcccaaa gataatattc cgccctttcc atgccaatta cctttctgaa cataggtctg
     1981 accgacattt gacgaactat tattgagaat aacatgaccg tttttctcca gagtcatgtt
     2041 acctgtgaca atgacggggt caggcaaaaa aggggcatta atattattga ttagcacagt
     2101 cccactattt aataaagtgt taacagtttg gttcatgcct gaaaaattca aggtagcacc
     2161 tttattaaca ataacaccag cggtacgtgt catagcttca actgtcccca tttttagaat
     2221 gcctgcattg atgttggtga aaccagagta ggtatttttt tccgccaaaa tgagagttcc
     2281 ctcccctgat tttgttaggg attttccgtc ccagctattt atattatgat ggctgagatt
     2341 atctgccaga atagtactaa cagtaagttc agcgttttct ttcagattga actctccatg
     2401 actgtcgcca tctgtatcat tccagctcaa accgtagatc aatttattct ggtcactgat
     2461 atagcttttt gtatagtcaa tagcaccagt agtgagtaac ggattgatga tagatatatt
     2521 agaaacactg aaacctgtta tccctttatc tgcacttagc agtttatagt ccttctgcgc
     2581 ccagttattt ataatagctt ccacttttac attgttgcca aatgacatat ttccagaagc
     2641 ttccagacta ctgattccag ctaaatttaa agaactgttc tcaccaaaag aaacgtcacc
     2701 tgaaacagtg aatttgttac ctgatagtga aacagttgta caatcaccag cacataaatc
     2761 accttcaata gttggtgtgg tcccagtgta atcattgttg ataattttca gaatggatga
     2821 attgttaacc tgtacatcac cagtaatgac agaaccttca tggagtatta atgaattgtt
     2881 tccaccagtg atgtggatag cattacctgc tacattttgt gttccatcat cttctttacc
     2941 tgaaattata tatccgctat tagcaatggt catattgcta ccatttatag catcccccca
     3001 atgtccacca ttaccgccta aaatagctcc attgttgatt atagacaggt tatctcctgt
     3061 gatagcatta ccaccgtagc catcataacc ataaccgtaa gcagcacctc cgttgcctcc
     3121 agcgaacgtg cccttattga ttacagagag gctgcttcct gtgatggcat cacctccatt
     3181 accgccatca ccctcaccat aattgttacc tccattacca ccggaaatag tccctttgtt
     3241 gattatgggt agattgactc ctgtgacggc atcacctcca ttccccccat cactatcact
     3301 ataactatcg ccaccatgtc cacctgaaat aatttctcca tttattataa acagattgtc
     3361 tcctgtaaca gcctcaccac catcaccgtt atggtcagca ccgctaccgc cgctaccacc
     3421 aagaatcatg ccttgattga ttatagacaa gtcagatccg gtaatagagt caccaccatt
     3481 accgccacaa ctattaccat cattattatc accagcacca ccatgacctg cgctaatcat
     3541 aatatcagag ttaatccgta gcttagttct agatgcatga gaggggttgg aagaagttat
     3601 ataccacgca tccattccat cttcaccagc tcccaaggga gaaagtccat caacaggtgt
     3661 taataatttt tcataattgt cctctgaaaa atgaagttct tgagtacccg aaagaggaat
     3721 agcaaaagct attggccccc cgagcaacag ggatgcacca acaacaaaag gagaaaaaga
     3781 caatttgcat gttttttcct ttaccgtagg taattctccg gcccccccct gtgaacattg
     3841 ggtcatatta caaaaaaatt tgtgaatttg attcatgcac tatattatca gtaagtggtt
     3901 gataaacccc taaaaagatt ccgttttatc cggaataaag ggacgatata tgcaaaaaca
     3961 tattaaacaa agcctcaacc aaaacatgag agctgtgtta tatatcaatg caacgggaga
     4021 aatttacctg tgtcttgctt tgaaaaaaag agggggaaag ctatatgttc gataataaat
     4081 taataatttt actatgatag tctgcttcag accgcaactt ttcagttctt tctgtacgct
     4141 tgcccaaagt ttaaccactt tgtttttcta tacacccaat ggttctatct gccccttgat
     4201 gttaatcccc aaagccaata acatgaatgt attcatatca caactttcct accagactct
     4261 ccaatgcaat aactgcataa ttgtttttag tggaaatgta tcgttttctt aaagagaaga
     4321 ataacattcc attttattat gttcccaatt taattatatt aatatatatt ggcatcaata
     4381 ggaaaataca tcaggagaaa tcaaatgcag acatcaaaca taactaacca tgaaagaaat
     4441 gactcatcat ggatgtcaac tgtcaaatca actacagaag taagttggaa taaattgagt
     4501 ttttgtgacg ttttactgaa aataataact ttcggaatat attctccaca cgaaactcta
     4561 gccgaaaaat atagcgaaaa aaaactaatg gatagtttct caccatcact atcacaagat
     4621 aaaatggacg gagagtttgc ccacgcaaac atagatggca tttcaatacg gctatgcctt
     4681 aacaaaggca tatgttcggt tttttatctt gatggagata aaatacaatc cactcaactt
     4741 tcatcaaaag aatataataa cttgttaagt tcactccccc ccaaacaatt caacttagga
     4801 aaagtacata ctattacagc tccggtatca gggaatttta aaactcacaa gcctgcacca
     4861 gaagttattg aaacagccat taattgctgt acatcgataa taccaaacga tgattacttt
     4921 cccgttaaag acactgattt taatagtgtt tggcatgata tttacagaga tattcgagca
     4981 tctgacagca atagtacaaa aatatatttc aacaatattg aaataccact aaaattaatt
     5041 gcagatctaa ttaatgagtt aggaattaat gaattcattg atagcaaaaa agagcttcaa
     5101 atgctaagtt acaaccaatt aaataaaata atcaattcaa acttccccca acaagaccta
     5161 tgttttcaaa cagaaaaact tttgtttact tcactctttc aagaccctgc atttatatcc
     5221 gcactaacat ctgcattctg gcaatctctt cacatcacgt cttcctctgt ggaacacata
     5281 tatgcccaaa ttatgtccga aaacatagaa aacaggctta atttcatgcc tgaacaacga
     5341 gttattaaca attgtggaca tattataaaa atcaacgcag ttgtccctaa aaacgacact
     5401 gcaatctctg cctctggggg gagggcttac gaagttagct catcaatatt accatcgcac
     5461 ataacatgta atggtgttgg tattaataaa atagaaacaa gctatctcgt tcatgctgga
     5521 acacttccaa gcagtgaagg gcttcgcaat gctatccctc cagaaagtcg tcaagtatca
     5581 tttgccatca tatctcctga tgtttaagcc aatattatat tggctgccag actgtaaaaa
     5641 aacgttcttc agaacagctc tataaactat taaaccaaag ctaaaaggat ggggaccaat
     5701 agaatctcag cccatccttt ttatatcatt atgaaccgcc ccgg
//
LOCUS       DAJTOR010000213         5731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTOR010000213 DAJTOR010000000
VERSION     DAJTOR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.213"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(530..901)
                     /locus_tag="OO249_003774"
     CDS             complement(530..901)
                     /locus_tag="OO249_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637899.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(962..1459)
                     /locus_tag="OO249_003775"
     CDS             complement(962..1459)
                     /locus_tag="OO249_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637900.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1535..2323)
                     /gene="aadA1"
                     /locus_tag="OO249_003776"
     CDS             complement(1535..2323)
                     /gene="aadA1"
                     /locus_tag="OO249_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5637901.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2381..2905)
                     /gene="sat2"
                     /locus_tag="OO249_003777"
     CDS             complement(2381..2905)
                     /gene="sat2"
                     /locus_tag="OO249_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5637902.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(3000..3473)
                     /locus_tag="OO249_003778"
     CDS             complement(3000..3473)
                     /locus_tag="OO249_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5637903.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3805..4341
                     /locus_tag="OO249_003779"
     CDS             3805..4341
                     /locus_tag="OO249_003779"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR5637904.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            4456..4782
                     /locus_tag="OO249_003780"
     CDS             4456..4782
                     /locus_tag="OO249_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5637905.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(4970..5209)
                     /locus_tag="OO249_003781"
     CDS             complement(4970..5209)
                     /locus_tag="OO249_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5637906.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1492 a         1376 c         1273 g         1590 t
ORIGIN      
        1 ttcccaggcc acttccctaa tattccagcc gctagaaaca agatttcgta cgacttcatc
       61 aacttttttg ctttccaact tgtcacaaag tgcataggca agagctttgg cggcagacac
      121 gtgcgtttcc tcttaaacat aggtcgaacg gatacccgag gcaaagcgga gcattagtcc
      181 caaagcatct ccggtagatc cggaggctcc atactgaagg catggcatct tgggcattga
      241 tatttgaaac ttctctctag atagcaggga tttcttggcc ttgcctcatg ctgatagctt
      301 gtaaatatca cacgatcctt agccccacaa agtgagcata ctccgtagct ttcatttata
      361 gaaatcgtcg ttagattgtc gcagcatgtg cacgttccga ctatttgctc aggaagcatc
      421 gccatacctt ggcctaggta aagaagatca gactcgtagc cacatcggtg gcaactgagc
      481 ttgtacgcaa tgcccatctg gttgtctcac ctgaatcgta acgtcagagc tattcatcca
      541 tccattcggc atcaatgtat gccactaagc cgaccagctg accgtttgga ctgaagtatg
      601 ttgcctgcac cccacttgac atctcatcaa taccaccgca ctgcgctagg cgccggtccc
      661 acttgcccac agaagcgtca ggatcggctg agttccccaa gacccttgtg agccatgcct
      721 cctttaactc aacctctcga atgattgcgt cggccagctc ctccgcgtta tctagttcga
      781 catagtctgg aattcggacg tacgttctga acacagctgg atacttactt gggcgattgt
      841 catacatgac atcaacaatg tacccgtttg tgtaaccgtc tcttggaggt tcgtatgaca
      901 ctagtggttc ccctcagctt gcgactagat gttgaggcct aacattttat tagagagcag
      961 gctagttgct tagatacatg atcttcaggc cgttatctgt cagggcaagc gaaaattggc
     1021 catttatgac gaccaatgcc ccgcagaagc tcccatcttt gccgccatag acgccgcgcc
     1081 ccccttttgg ggtgtagaac atccttttgc cagatgtgga aaagaagttc gttgtcccat
     1141 tgttggcaat gacgtagtag ccggcgaaag tgcgagaccc atttgcgcta tatataagcc
     1201 tacgatttcc gttgcgacta ttgtcgtaat tggatgaact attatcgtag ttgctctcag
     1261 agttgtcgta atttgatgga ctattgtcgt aattgcttat agagttgtcg tagttgcttg
     1321 gagaaatgtc gtagttggat ggggagtagt catagggaag acgagcttca tccactaaaa
     1381 caattggcag gtcagcaagt gcctgccccg atgccatcgc aagtacgagg cttagaacca
     1441 ccttcaacag atcgcgcata gtcttcccca gctctctaac gcttgagtta agccgcgccg
     1501 cgaagcggcg tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct
     1561 cgcctttcac gtagtgaaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
     1621 cttgtccaag ataagcctgc ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     1681 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
     1741 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     1801 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     1861 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     1921 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
     1981 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
     2041 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
     2101 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
     2161 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
     2221 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
     2281 ctgatagttg agtcgatact tcggcgatca ccgcttccct catgatgttt aactcctgaa
     2341 ttaagccgcg ccgcgaagcg gtgtcggctt gaatgaattg ttaggcgtca tcctgtgctc
     2401 ccgagaacca gtaccagtac atcgctgttt cgttcgagac ttgaggtcta gttttatacg
     2461 tgaacaggtc aatgccgccg agagtaaagc cacattttgc gtacaaattg caggcaggta
     2521 cattgttcgt ttgtgtctct aatcgtatgc caaggagctg tctgcttagt gcccactttt
     2581 tcgcaaattc gatgagactg tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa
     2641 tgtgttcgat agaggctaga tcgttccatg ttgagttgag ttcaatcttc ccgacaagct
     2701 cttggtcgat gaatgcgcca tagcaagcag agtcttcatc agagtcatca tccgagatgt
     2761 aatccttccg gtaggggctc acacttctgg tagatagttc aaagccttgg tcggataggt
     2821 gcacatcgaa cacttcacga acaatgaaat ggttctcagc atccaatgtt tccgccacct
     2881 gctcagggat caccgaaatc ttcatatgac gcctaacgcc tggcacagcg gatcgcaaac
     2941 ctggcgcggc ttttggtaca aaagccgcgc caggtttgcg aatccgttgc tgccacttgt
     3001 taaccctttt gccagatttg gtaactataa tttatgttag aggcgaagtc ttgggtaaaa
     3061 actggcctaa aattgctggg gatttcagga aagtaaacat caccttccgg ctcgatgtct
     3121 attgtagata tatgtagtgt atctacttga tcgatcaggc ttttgtatat ctccccacca
     3181 cctgaaacaa tgacatgatc cgttattttc tttaggttgg ttaaagcatc tttaattgat
     3241 ggaaagatca atacgttctc attgtcagat gtaaaacttg aacgtgttac gaccgcatac
     3301 tttcggttgg gtaatgctcc cattgattca aaagtcttgc gtccaaccaa cagccattgg
     3361 ttataggtaa tagctttaaa caggagctgt tcacctttgg cactccatgg aatatcaggg
     3421 ccattcccga taactccatt cttcgatata gctaccatta gtgatagttt cacaattctt
     3481 cctcagaggt taacatttta ttagtaagca tgctcgtttt gtcacccgct gatgcttacc
     3541 gttaattaat ataatcactt gtattaaata gactttcttt aaaaatacaa gacactctgt
     3601 tattacaaat cgtgcatgcc gtctatcctg cttgcacgat gcacattatc tcacccaaag
     3661 aactcataac attttaattt atttagcatt aaattaaaac tctaccggta aacaagcatc
     3721 tctaggcgta aaaaacaggc acttttttaa atcccagcta gaataggctg tataggcaga
     3781 cagttgcaag acaaggagcg ttttatgtct aacagtccat ttttaaattc tatacgcacg
     3841 gatatgcgac aaaaaggtta tgcgctgaaa actgaaaaaa cttacctgca ctggattaag
     3901 cgttttattc tgtttcacaa aaaacgtcat cctcagacca tgggcagtga agaggtcagg
     3961 ctgtttttat ccagcttagc aaacagcaga catgtagcca taaacacgca gaaaatcgct
     4021 ttaaatgccc tagctttttt gtacaacagg tttttacaac agccgttggg cgatattgat
     4081 tatatccctg caagcaagcc tagacggcta ccctctgtta tctctgcaaa tgaagtgcaa
     4141 cgcattttgc aggttatgga tactcgcaac caagttattt ttacgctgct gtatggtgca
     4201 ggtttgcgca ttaatgaatg cttgcgtttg cgggttaaag attttgattt tgataatggc
     4261 tgcatcactg tgcatgacgg taagggtggg aaaagcagaa acagcctact gcccacgcgc
     4321 ctaatcccag caataaaata actcattgag caagcgcggc ttattcagca agacgacaac
     4381 ttacaaggcg tagggccatc gctgcctttt gctttagatc acaaataccc ttctgcttat
     4441 cgacaagcgg cgtggatgtt tgtctttccc tccagcacgc tctgcaacca cccgtataac
     4501 ggcaaattat gccgccatca tctgcatgac tccgttgcgc gaaaggcatt gaaggcagcc
     4561 gtacaaaaag caggcatcgt tagcaagcgt gtcacttgtc atacatttcg tcactcgttt
     4621 gctacgcatc tattacaagc ggggcgtgat attcgcactg tgcaagaact cttagggcat
     4681 aacgatgtta agaccacgca aatctatacg catgtgttgg gtcagcattt tgccggcacc
     4741 accagtcctg cggatggact gatgctactt atcaatcagt aaaagtagat aaatatcttt
     4801 atgtagccct gagtagcccc tgatactttt tatggtgaaa aagataaaca atcatagagg
     4861 ggataaatat gagccagttc taccattgtt cagataagtt taagcaagat actgtcaatc
     4921 acagtgacca ttcatggtca ttacacttcc tccagttgcc agacaatgcc taacataaaa
     4981 acgcaaaagc ccaaaagtca gagcttaggt gttctaggcg ttttctaagc gctgaagaaa
     5041 tacgtcttac cgtagattgc gtgcgtttct gaagcacatt ttccttatac atggctttat
     5101 cccaggcagc ttcatcaagc tcttggagta acaagccagt aataatgcga cactctcggg
     5161 tttgtagcga gccgctggtg agatcggagt tatagcggaa aggggtcatt tgtaattatt
     5221 tgtgggtgga ggatagttaa cttattattg tagttggaag aggctgcttt ccaaacatta
     5281 gcacctcgta atgcttgtta ctgtatatta atttatgctc ttagcgcatg ttaatgggct
     5341 attacaatgc ataatgtcca catcaagata actgtggctt aacacctgta caagcgaaaa
     5401 aaatcaatca cctgtccgga aatacgggat cactgtaaag tgttggtgag gcagtctagg
     5461 agggggattt aatttgggat actgatgaaa ggattatgat cagttggata taggatcgta
     5521 ctaagctgag cggcaggtta gctagattag ttaaccagat aagtgaaatc tagttccaaa
     5581 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
     5641 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
     5701 ctattttgtc cgcccacagg gctgccgacc g
//
LOCUS       DAJTOR010000214         5704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTOR010000214 DAJTOR010000000
VERSION     DAJTOR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.214"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..183)
                     /locus_tag="OO249_003782"
     CDS             complement(<1..183)
                     /locus_tag="OO249_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR5637907.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGE"
     gene            709..1404
                     /gene="minC"
                     /locus_tag="OO249_003783"
     CDS             709..1404
                     /gene="minC"
                     /locus_tag="OO249_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5637908.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1428..2240
                     /gene="minD"
                     /locus_tag="OO249_003784"
     CDS             1428..2240
                     /gene="minD"
                     /locus_tag="OO249_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5637909.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2244..2510
                     /gene="minE"
                     /locus_tag="OO249_003785"
     CDS             2244..2510
                     /gene="minE"
                     /locus_tag="OO249_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5637910.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2989..3108)
                     /locus_tag="OO249_003786"
     CDS             complement(2989..3108)
                     /locus_tag="OO249_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5637911.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3069..3254)
                     /locus_tag="OO249_003787"
     CDS             complement(3069..3254)
                     /locus_tag="OO249_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637912.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            3355..3528
                     /locus_tag="OO249_003788"
     CDS             3355..3528
                     /locus_tag="OO249_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637913.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVRATYLIPDI"
     gene            3530..3874
                     /locus_tag="OO249_003789"
     CDS             3530..3874
                     /locus_tag="OO249_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5637914.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3878..4213
                     /locus_tag="OO249_003790"
     CDS             3878..4213
                     /locus_tag="OO249_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5637915.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4270..>5704)
                     /locus_tag="OO249_003791"
     CDS             complement(4270..>5704)
                     /locus_tag="OO249_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5637916.1"
                     /translation="VVTHLTNSDSIINLSYDDGQTFTQGKTLTVKGNYVGNNGQLNIR
                     TVLGDDKSATDRLIVEGNTSGSTTVYVKNAGGSGAATLNGIELITVNGDESPAEAFRQ
                     GDARIAAGAFEYQLKQQGKNWYLTSYQSVNEEDNSSEGNSEPTETPTPVLRPEAGSYV
                     ANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTS
                     HRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYCAGLY
                     ATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKS
                     FGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQE
                     KNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGNLNPAAS
                     VWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT         1558 a         1325 c         1325 g         1496 t
ORIGIN      
        1 ttcgccgaga tatttccttg ttaactctgg ggaaatacca tctgagttca cacaaatata
       61 cgggtcgcaa acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc
      121 cattgatgag aacatcatcc ccacacctga caatccgata taaaaaatac gcatcatatt
      181 catagcacat cctgataatc caaccgtaca acacttatgc tcatttcatc atcaatccgt
      241 aatcaaaacc gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct
      301 gcggtttcta tgattatgat agagcaattc tatggcggat attaagataa cgggtatctg
      361 ttctttaatg atgctgcaaa aaatggaaag ttaagaaagc ctggcgtggt gaaaaaatac
      421 acttgcctca atactggata tttaaacagg tctggagatg ctgtcacgat tagggtgcgg
      481 aaaaaggaag cgtaaataag aacaatcaaa agtacgacgg caatgggttg attgacaagg
      541 gtatttttta agctatgaat cagcgccatt tatcacagaa tagactttta ctctgaataa
      601 atgggagggt gacttgcctc aatataatcc agactataac atgccttata gtcttcggaa
      661 catcatcgcg cgctggcgat gattaatagc taattgagta aggccaggat gtcaaacacg
      721 ccaatcgagc ttaaaggcag tagcttcact ttatctgtgg ttcatctgca tgaggcagaa
      781 cctaaggtta tccatcaggc gctggaagac aaaatcgctc aggcccccgc atttttaaaa
      841 catgcccccg ttgtactcaa cgtcagtgca ctggaagacc cggtaaactg gtcagcgatg
      901 cataaggcgg tgtcggcaac cggtttgcgg gttattggcg taagtggctg caaagatgcg
      961 caacttaaag ccgaaattga aaagatgggg ctgcctatcc tgacggaagg aaaggaaaaa
     1021 gcgccgcgtc cagctcccgc accacaggct ccagcgcaaa atacaacgcc ggtcacaaaa
     1081 acgcgtttaa tagatacccc ggtgcgttcc ggtcagcgta tttatgctcc acaatgtgat
     1141 ctgattgtta caagccacgt tagcgctggg gccgaattga ttgccgatgg gaacattcat
     1201 gtctatggca tgatgcgcgg tcgtgcgctt gcaggggcaa gtggtgaccg ggaaacgcaa
     1261 atattttgta cgaacctgat ggcggaactg gtgtccatcg caggtgaata ctggctgagt
     1321 gatcaaatcc cagcagaatt ttatggcaaa gcggcgcgac tgcagttagt cgaaaacgct
     1381 ttgaccgttc aaccgttaaa ttgatccctt tttaacaagg aatttctatg gcacgcatta
     1441 ttgttgttac ttcgggcaaa gggggtgttg gtaagacaac ctccagcgcg gccatcgcca
     1501 ctggtttggc ccagaaggga aagaaaactg tcgtgataga ttttgatatc ggcctgcgta
     1561 atctcgacct gattatgggt tgtgaacgcc gggtcgttta cgatttcgtc aacgtcattc
     1621 agggcgatgc aacgctaaat caggcgttaa ttaaagataa gcgtactgaa aatctctata
     1681 ttcttccggc atcgcaaaca cgcgataaag atgccctcac ccgtgaaggg gtcgccaaag
     1741 ttcttgatga tctgaaagcg atggattttg aatttatcgt ttgtgactcc ccggcaggga
     1801 ttgaaaccgg tgcgttaatg gcactctatt ttgcagacga agccattatt accaccaacc
     1861 cggaagtctc ctcagtacgc gattctgacc gtattttagg cattctggcg tcgaaatcac
     1921 gccgcgcaga aaatggcgaa gagcctatta aagagcacct gctgttaacg cgctataacc
     1981 caggccgcgt aagcagaggt gacatgctga gcatggaaga tgtgctggag atcctgcgca
     2041 tcaaactcgt cggcgtgatc ccagaggatc aatcagtgtt gcgcgcctct aaccagggtg
     2101 aaccggtcat tctcgacatt aacgccgatg cgggtaaagc ctacgcagat accgtagaac
     2161 gtctgttggg agaagaacgt cctttccgct tcattgaaga agagaagaaa ggcttcctca
     2221 aacgcttgtt cggaggataa gttatggcat tactcgattt ctttctctcg cggaagaaaa
     2281 acacagccaa cattgcaaaa gaacggctgc agattattgt tgctgaacgc cgtcgcagcg
     2341 atgcagaacc gcattatctg ccgcagttgc gtaaagatat tcttgaggtc atttgtaaat
     2401 acgtacaaat tgatcctgag atggtaaccg tacagcttga gcaaaaagat ggcgatattt
     2461 ctattcttga gctgaacgtg accttaccgg aagcagaaga gctgaaataa gcccgctgta
     2521 aaaacgcatt tatcttcaag gcagagtcat ttctgccttg agtttttcat ccctctcatc
     2581 cacgttgtgg taaagcggcg agtattcttg ctgatactcc tcattgctat ttccacctcc
     2641 ccctttacac cttaaggctg ttcccacgca cagaacaaaa tgtttatgcc tggttgagta
     2701 aataacctca ttgttagtgt ggatacactg ggggatgata accccgcaat ttcattattc
     2761 attgttctcc cataggatga ggtttcccgc cgtaactggc gggctttttt tgccccagaa
     2821 ttttcccttt tagccccctg taaacgaaaa ctgcgccgaa gcgcagttta atcgttgatg
     2881 gtcagaacga ccagttcacg atatatactg cgggtatcga ctgatttcga ttccatgcca
     2941 ttggataaat gatagtagcg tctaagtgac ctaagctctg ctgcaacatc agcccgcagg
     3001 caccagacca ggggattcat cctgaagaga cagcgcaggt gtattgtgtt caccactcac
     3061 caaagacatc atgatgaaat gatgatatcc cgcataagaa agaggcattt tttaaacgca
     3121 gtgcgctgaa gtgtagttgg ataaaaaatc actcttttca gataatactg tcgtacgctt
     3181 atctttcgac aatgaggccg tcggcaaaat aaaatgacta atgtaatcgt tgctgatgaa
     3241 tatcttctgc tcacataaaa atcacacaat aatattgata aatcagatta tcttaccttt
     3301 acaacattgc gttaacagtc aatgacactt cttgaataaa aatattgcca atcaatgagt
     3361 tattcctcat ttaagattat tctcattcac gtaaaaaaca taattcctat catcacagcc
     3421 acgctcattt tgaattatct gaataatagc gagcgttctt tagttaagca gatcttaatt
     3481 gaagacgaaa ttattgtgcg tgcaacttac ttaataccgg acatctaagt tgaagaaaaa
     3541 aatacttgcc ttcgggctta tctcagcatt gttctgctcc acgccagcga tggcggatat
     3601 gaatcgcacc acgaaaggtg cattgctagg cgcaggcgtt ggtttactta ctggcaatgg
     3661 tgttaatggc gtactgaaag gtgcagctgt tggcgctggt gttggtgcag taacagaaaa
     3721 aggccgcgac ggtaaaaatg cgcgtaaagg tgcaaaagta ggtgccgccg ttggtgctgt
     3781 cactggcgtt ctgaccggca atggcctcga aggggcgatt aaaggtgccg tgattggcgg
     3841 tacgggtggt gcaattctgg gtaaaatgaa ataaggaatg atgatgaaaa aatttgcttt
     3901 actggcgggc ctgtttgttt tcgcaccaat gacctgggcg caggactaca atattaaaaa
     3961 cggtttaccg tcagaaacct atattacttg cgcagaagcc aacgaaatgg caaaaactga
     4021 cagcgcacag gttgcagaaa ttgttgcggt aatgggtaat gccagcgttg ccagccgtga
     4081 tttaaaaatt gagcaatctc cggaactgag cgccaaagtg gtagaaaaac tgaatcaggt
     4141 ttgcgctaaa gacccgcaaa tgctgttgat cacggcaatt gatgacacca tgcgtgctat
     4201 cggcaaaaaa taatatttca tatgtaccgc gtcgatgaat acgacgcggt tttttagcta
     4261 acggcgggat cagaacttat atttcaggcc caccatcact gcggtgtcat tgtagccatt
     4321 atcacccagc tgcacgccca cattccccca gacgctggcc gctggattca ggttgccatt
     4381 tacgccaagt ttaatttccc ccaaattact cgccccatct tgttcgactt tcacaccgtt
     4441 cattgagaca gcaaaatctt tactgttatg gatccagttc atttcaacaa aaggttcaaa
     4501 gtcgtcaccg tgcggaccgc tgtttttctc ctgagtacga ataaaggtgc ggaaaccaag
     4561 tcgggtctgg atgttgttat ttgcgtcatt ctcaatacgt gagccattgg cttcggtgtg
     4621 cgcattatga tcgacgccca tccagatagc ctgtgcctgt ggctggaata tccacgtgta
     4681 tttcgccgct tccagaccaa aggatttatt cagggcaaag ccgtaacccg cttccagcga
     4741 gacggttgcg cctttcgcac tataggattc ataggctaat tcatcccctt tcaccgagtt
     4801 tttaaaccag ctatattgcg cccaggcgtc aatgtatgcg ccttttttac tgatgtcatc
     4861 ggcaaaccaa gtggcataca gccctgcgca atagcctctg acactccctt tcgaacgata
     4921 atccgacacg ctggaatgag ttgagttgta gtcgcgggca taaccagcca tcactcccaa
     4981 acgccaactg tcactgtcgg taaaaccacc ggttaacaga tcggccccta actgcgagac
     5041 gtagcgatgc gaggtcgttc tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc
     5101 aatttgacgt agccaaagtc ggcttgaacg ctcctgttca gttacaggat cgatgtagcg
     5161 catttcaccc gcacggtcgt tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt
     5221 ggcgacgtaa cttccagctt cggggcgcag gacgggggta ggcgtttctg ttggttctga
     5281 atttccctct gagctgttat cctcctcatt cactgactga taacttgtca aataccagtt
     5341 tttgccttgt tgttttagtt gatattcgaa agctccagct gcaatccttg catcaccttg
     5401 tctgaaggct tctgctggag attcatcgcc attcacagtt atgagttcga tcccgtttag
     5461 cgtggccgcg ccgcttcctc cagcattttt cacatagacg gtagttgaac ccgaagtatt
     5521 accctcaaca ataagtctgt ccgtagccga tttatcatca cctaatacgg tgcggatatt
     5581 aagctgacca ttattaccga cataattacc ttttacggtt aatgtttttc cttgggtaaa
     5641 tgtttgacca tcatcatacg aaagattgat aatactgtct gaattagtca gatgggtaac
     5701 aacg
//
LOCUS       DAJTOR010000215         5673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTOR010000215 DAJTOR010000000
VERSION     DAJTOR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.215"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..564
                     /locus_tag="OO249_003792"
     CDS             <1..564
                     /locus_tag="OO249_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5637917.1"
                     /translation="GEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVT
                     GMAEDMQSLVGGTVVRRKVYAHFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVS
                     ASFVLSTPTETDGAVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKC
                     SKCLSGCKFRNNVGNFGGFLSINKLSQ"
     gene            570..1313
                     /locus_tag="OO249_003793"
     CDS             570..1313
                     /locus_tag="OO249_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5637918.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFYREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            1250..1882
                     /locus_tag="OO249_003794"
     CDS             1250..1882
                     /locus_tag="OO249_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5637919.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TCFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1943..5341
                     /locus_tag="OO249_003795"
     CDS             1943..5341
                     /locus_tag="OO249_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5637920.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRVLQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTAETTYRFTQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPVAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGAGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGNVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            5408..>5673
                     /locus_tag="OO249_003796"
     CDS             5408..>5673
                     /locus_tag="OO249_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5637921.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQITRYSDTRWH"
BASE COUNT         1289 a         1438 c         1770 g         1176 t
ORIGIN      
        1 ggtgagccgg tcacctggca ggggcgacag tatcagccgt atcccattca ggggagcggt
       61 tttgaactga atggcaaagg caccagtacg cgccccacgc tgacggtttc taacctgtac
      121 ggtatggtca ccgggatggc ggaagatatg cagagtctgg tcggcggaac ggtggtccgg
      181 cgtaaggttt acgcccattt tctggatgcg gtgaacttcg tcaacggaaa cagttacgcc
      241 gatccggagc aggaggtgat cagccgctgg cgcattgagc agtgcagcga actgagcgcg
      301 gtgagtgcct cctttgtact gtccacgccg acggaaacgg atggcgctgt ttttccggga
      361 cgtatcatgc tggccaacac ctgcacctgg acctatcgcg gtgacgagtg cggttatagc
      421 ggtccggctg tcgcggatga atatgaccag ccaacgtccg atatcacgaa ggataaatgc
      481 agcaaatgcc tgagcggttg taagttccgc aataacgtcg gcaactttgg cggcttcctt
      541 tccattaaca aactttcgca gtaaatccca tgacacagac agaatcagcg attctggcgc
      601 acgcccggcg atgtgcgcca gcggagtcgt gcggcttcgt ggtaagcacg ccggaggggg
      661 aaagatattt tccctgcgtg aatatctccg gtgagccgga ggcgtatttc cgtatgtcgc
      721 cggaagactg gctgcaggca gaaatgcagg gtgagattgt ggcgctggtc cacagccacc
      781 ccggtggtct gccctggctg agtgaggccg accggcggct gcaggtgcag agtgatttgc
      841 cgtggtggct ggtctgccgg gggacgattc ataagttccg ctgtgtgccg catctcaccg
      901 ggcggcgctt tgagcacggt gtgacggact gttacacact gttccgggat gcttatcatc
      961 tggcggggat tgagatgccg gacttttatc gtgaggatga ctggtggcgt aacggccaga
     1021 atctctatct ggataatctg gaggcgacgg ggctgtatca ggtgccgttg tcagcggcac
     1081 agccgggcga tgtgctgctg tgctgttttg gttcatcagt gccgaatcac gccgcaattt
     1141 actgcggcga cggcgagctg ctgcaccata ttcctgaaca actgagcaaa cgagagaggt
     1201 acaccgacaa atggcagcga cgcacacact ccctctggcg tcaccgggca tggcgcgcat
     1261 ctgcctttac ggggatttac aacgatttgg tcgccgcatc gaccttcgtg tgaaaacggg
     1321 ggctgaagcc atccgggcac tggccacaca gctcccggcg tttcgtcaga aactgagcga
     1381 cggctggtat caggtacgga ttgccgggcg ggacgtcagc acgtccgggt taacggcgca
     1441 gttacatgag actctgcctg atggcgctgt gattcatatt gttcccagag tcgccggggc
     1501 caagtcaggt ggcgtattcc agattgtcct gggggctgcc gccattgccg gatcattctt
     1561 taccgccgga gccacccttg cagcatgggg ggcagccatt ggggccggtg gtatgaccgg
     1621 catcctgttt tctctcggtg ccagtatggt gctcggtggt gtggcgcaga tgctggcacc
     1681 gaaagccaga actccccgta cacagacaac ggataacggc aaacagaaca cctgtttctc
     1741 ctcactggat aacatggttg cccagggcaa tgttctgccg gttctgtacg gtgaaatgcg
     1801 cgtggggtca cgcgtggttt ctcaggagat cagcacggca gacgaagggg acggtggtca
     1861 ggttgtggtg attggtcgct gatgcaaaat gttttatgtg aaaccgcctc cgggcggttt
     1921 tatcgtttat ggagcatgac gaatgggtaa aggcagcagt aaggggcata ccccgcgcga
     1981 agcgaaggac aacctgaagt ccacgcagct gctgagtgtg atcgatgcca tcagcgaagg
     2041 gccggttgaa ggtccggtgg atggattaaa aagcgtgctg ctgaacagta cgccggtgct
     2101 ggacagtgag gggaatacca atatatccgg cgtcacggtg gtgttccggg ccggtgagca
     2161 ggagcagaca ccgccggagg gatttgaatc ctccggttcc gagacggtgc tcggtacaga
     2221 agtgaaatat gacacgccga tcacccgcac cattacgtct gcaaacatcg accgtctgcg
     2281 ctttaccttc ggtgtacagg cactggtgga aaccacctca aagggggaca ggaatccatc
     2341 ggaagtccgc ctgctggttc agatacagcg taacggtggc tgggtgacgg aaaaagacat
     2401 caccattaag ggtaaaacca cttcacagta tctggcctcg gtggtggtgg ataacctgcc
     2461 gccgcgcccg ttcagtatcc ggatgcgcag gatgacgccg gacagcacca cagaccagct
     2521 gcagaacaaa acgctctggt cgtcatacac cgaaattatc gatgtgaaac agtgctaccc
     2581 gaacacggca ctggtcggcg tgcaggtgga ctcggagcag ttcggcagcc agaaggtgag
     2641 ccgtaattat catctgcgcg ggcgtgttct gcaggtgccg tcgaattata acccgcagac
     2701 gcggcaatac agcggtatct gggacggaac gtttaaaccg gcatacagca acaacatggc
     2761 ctggtgtctg tgggatatgc tgacccatcc gcgctacggc atggggaaac gtcttggtgc
     2821 ggcggatgtg gataaatggg cgctgtatgt catcggccag tactgcgacc agtcagtgcc
     2881 ggacggcttt ggcggcacag agccgcgcat cacctgtaat gcctggctga ccacgcagcg
     2941 taaggcgtgg gatgtgctca gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa
     3001 cgggcagacg ctgacgttcg tgcaggaccg accatcagat aaggtgtgga cctataaccg
     3061 cagtaatgtg gtgatgccgg atgatggcgc gccgttccgc tacagcttca gcgccctgaa
     3121 ggaccgccat aatgccgttg aggtgaactg gattgacccg gataacggct gggagacggc
     3181 gacagagctt gtggaggaca cgcaggccat tctccgttac ggtcgtaacg tcacgaagat
     3241 ggatgccttt ggctgtacca gccgggggca ggcacaccgc gccgggctgt ggctgattaa
     3301 aacggagctg ctggagacgc agaccgtgga tttcagcgtc ggcgcagaag ggcttcgcca
     3361 tgtgccgggt gatgttattg aaatctgcga tgatgactat gcggggatca gcatcggcgg
     3421 gcgcgtgctg gcggtgaaca gccagacccg gacgctgacg ctcgaccgtg aaatcacgct
     3481 gccatcctcc ggtaccacgc tgataagcct ggttgacgga agtggcaatc cggtcagcgt
     3541 ggaggtccag tccgtcaccg acggcgtgaa ggtaaaagtg agccgtgttc ctgacggcgt
     3601 tgccggatac agcgtatggg ggctgaagtt gccgacgttg cgccagcgcc tgttccgctg
     3661 cgtgagtatc cgtgagaacg atgacggcac gtatgccatc accgccgtgc agcatgtacc
     3721 ggaaaaagag gccatcgtgg ataacggggc gcactttgac ggcgaccaga gcggcacggt
     3781 gaatggtgtc acgccgccag cagtgcagca tctgaccgcc gaagtcactg cagacagcgg
     3841 ggaatatcag gtgctggcgc gatgggacac gccgaaggtg gtgaagggcg tgagcttcct
     3901 gcttcgcctg accgtggcag cggatgacgg cagtgagcgg ctggtcagca cggcccggac
     3961 ggcggaaacc acataccgct tcacgcagct ggcgctggga cggtacacgc tgacagtccg
     4021 ggcggtaaat gcgtggggac agcagggcga tccggcgtcg gtatcgttca ggattgccgc
     4081 accggtagca ccgtcgcgga ttgagctgac gccgggctat tttcagataa ctgccacgcc
     4141 gcatcttgcg gtttatgatc cgacggtaca gtttgagttc tggttctcgg aaacgcggat
     4201 taccgatatc aggcaggttg aaaccacagc ccgctacctt ggcgcggggc tgtactggat
     4261 agccgccagt atcaatatca aaccgggcca tgattattac ttttatatcc gcagtgtgaa
     4321 caccgttggc aaatcggcat tcgtggaggc cgtcggtcgg gcgagcgatg atgcggaagg
     4381 ttacctggat tttttcaaag gcaagataac cgaatcccat ctcggtaaag agctgctgga
     4441 aaaagttgat ctgacggagg ataacgccag cagactggat gagttttcga aagagtggaa
     4501 ggacgctaac gataaatgga atgcgatgtg gggcgtcaaa attgagcaga ccaaagacgg
     4561 caaacattat gtcgcgggta ttggcctcag catggaggac acggaggaag gcaagctgag
     4621 ccagtttctg gttgccgcta accgtatcgc gtttattgac ccggcaaacg ggaatgaaac
     4681 gccgatgttt gtggcgcagg gcaaccagat attcatgaat gacgtgttcc tgaaacgcct
     4741 gacggctccc accattacca gcggtggaaa tccgccggca ttttccctga caccagacgg
     4801 aaagctgacc gctaaaaatg cggatatcag cggtaatgtg aatgcaaatt cagggacgct
     4861 caacaatgtc acgattaatg aaaactgtca gattaagggg aaactgtcag ccaatcagat
     4921 tgaaggcgat attgtcaaaa cggtcagcaa gtctttcccc cgcacgaaca gttatgccag
     4981 tggcaccatc acggtaagaa tcagtgatga tcagaaattt gaccggcagg tcatgatacc
     5041 gccagtgtta ttccgcggtg gtaagcatga gaatttcaac agtaataacc aacagtcata
     5101 ctggtattca acctgccggt taagagtgac ccgcaatggt caggagattt ttaatcagtc
     5161 cacgacggat gctcagggcg tattttcctc agttatagat atgcctgccg gacaggggac
     5221 gctgacactg acattcaccg tatcttcatc aggagcgaat aactggacac caacaaccag
     5281 tatcagcgat ctgctggttg tggtgatgaa aaaatccaca gcaggtatca gtatcagctg
     5341 aattttataa cccagaacgg gcgtcagaaa tgacgccttt tttattgcag aaaagcgaga
     5401 ggtaattatg cgtaaacttt atgccgccat tttgtccgca gccatttgtc tggccgtatc
     5461 cggtgcgcct gcatgggcgt ctgaacatca gtccacgctg agcgcggggt atcttcatgc
     5521 ccggacgaac gttcccggca gtgatgatct gaacgggatt aacgtgaaat accgttatga
     5581 gtttacggac acactgggga tggtgacgtc gttcagctat gcaggagaca agaatcgcca
     5641 gattacccgt tacagcgata cccgctggca tga
//
LOCUS       DAJTOR010000216         5669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTOR010000216 DAJTOR010000000
VERSION     DAJTOR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.216"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(21..1940)
                     /gene="dhaR"
                     /locus_tag="OO249_003797"
     CDS             complement(21..1940)
                     /gene="dhaR"
                     /locus_tag="OO249_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5637922.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            2168..3277
                     /gene="dhaK"
                     /locus_tag="OO249_003798"
     CDS             2168..3277
                     /gene="dhaK"
                     /locus_tag="OO249_003798"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5637923.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLGNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWDKLGESNVTEQNSNC"
     gene            3249..3881
                     /gene="dhaL"
                     /locus_tag="OO249_003799"
     CDS             3249..3881
                     /gene="dhaL"
                     /locus_tag="OO249_003799"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5637924.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            3892..5310
                     /gene="dhaM"
                     /locus_tag="OO249_003800"
     CDS             3892..5310
                     /gene="dhaM"
                     /locus_tag="OO249_003800"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5637925.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLYTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDYIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1419 a         1453 c         1399 g         1398 t
ORIGIN      
        1 ttagaaatcg tttctgtctt tcatacccgg cgcttaaact gccctgcatc aatgccatgt
       61 tgcttcattt tccgccacag cgtagtgcgg ccgattccaa gtaaagccga catttcctga
      121 atgcgaccgc ctgtgacctg ggctgcgtta ataattgcct ctttttcaac ttccgcaaat
      181 gacagactgg tggaaaggcg ggtggcgctg acatcatctg ttgcctgctc ggtaaacaga
      241 tgttccggca aatcactgac gcgaatgcgc ccattatcac tactcagagc aagattctcg
      301 atgacgctgt aaagttcaaa atcgttgcct ggccatgcac aagaaaccag gcgagcgagg
      361 gcatcgtcat caattttcag ccgcgtagag aagcgttttt caagactgcg taatttgtta
      421 ttcaccagcg ccggaatgct gccacgccgc atacgcagag gcgggatggt aatttcaaat
      481 gcatgcagcg cgtaatacag ctggcgacta aaacgatttt gttccaccag cattgcgagg
      541 tccgcggtcg ttgttgcaat cactttgaca tcaattggta ttaaacgccg cgcatccagt
      601 cgcgtgataa caccctgctt gataacctga agcaaagcag actgtaactc caccgccaga
      661 tattcaatct tttcaagaaa cagcgtgccg ccgtgcgcca gttccagccg actcagacgg
      721 ccattttcat tgtccgtacg atcgccacca ataaattctt ccgccagtgc cgcatcacca
      781 tataactcac aattgacggc gatataagga cctgcagcac gctcgctttc attatgaatt
      841 gcctgactta gcagtgcctt gcccacgccc tcttctccac aaagcaggac aggaaagcta
      901 ctgcgcgccg cctggcgacc aaaatgaatc aagcggcggg tttgcggatc gtcctgtggc
      961 atatgagcaa aggtatggct gacttttcct aattgactgg tcatcaactg ccgcatctgt
     1021 tccacaggat ggagcaacaa aataaagctg gttccctgcg tttctattat cggtttaagg
     1081 gttatcaccg catcaataaa ctgatgctga ctttcaaagg ttgcttctac gtgtttgagc
     1141 ggatgtgcct gttttattgc ttgttgcaat acggcgggta acgttaagag ttcagtgatt
     1201 gcccgtccct gacttgccgt cgcgtcaagg cgcaagaccc gcgccgcctg ggcattgata
     1261 aattgcaaat taccctgctc gtcccagcta atcacgccat catccatact ttctaacagg
     1321 gcattaagtt gatttaaatg acggttagtt tcagcgagca aactgtccgt cagcagtaaa
     1381 tttccgacct cgcgggcgat tgccaacgtc aacggcaaat cagctgcggt agtttgctca
     1441 accggacacg ccagcgctat tgttcccgtc aatcggccct tgctgtcaaa caacggcgtt
     1501 gcacaaaagg cccagttcca gagtgcctgt ttgaaatgtt gatcggccat cgttttcacg
     1561 gcctgaccag agatagccgc taacgatagc gcacaagtac caataattcc ctcggcgcaa
     1621 tacgtgccgt catggaatcc aagtgcactt agctgctgca aggtttgcgg atcgccatta
     1681 cggctgagaa tgcaggcggt ttcatcgagg ataaacagcg cacactctcg cggtgccata
     1741 tattcccagg catcttccag cgcagcctgc ccgagcgtca gcatcgcttt cttacgccga
     1801 taaatagaag caaatgtcac gccctgggcc tgatgtggta cgctccatgt ctcccgtttc
     1861 atcagcttat tgcatcgctc ccaggaggtt gcaattaagg gagatatgcc ccgaccatcg
     1921 ttgttaaaag cgccactcat atcccgcatc ccttatgaca aaaggtgttc cggctcgcaa
     1981 ttttaagtat taatacacgt aaaagtttca atatggaaca ttcattaaag catatgtttc
     2041 actatgaaac ataatttggg ctaattttct tttcccttgc ctgatgcaca atggattcat
     2101 cggcagtcag tggtcgccgt gtcgttgaac atcatccatg ccctaccgta attgctggag
     2161 caaaataatg aaaaaattga tcaatgatgt gcaagacgta ctggacgaac aactggcagg
     2221 actggcgaaa gcgcatccat cgctgacact gcatcaggat ccggtgtatg tcacccgagc
     2281 tgatgcccct gttgcaggaa aagtcgccct gctgtcgggt ggcggcagcg gacacgagcc
     2341 gatgcactgt gggtatatcg gtcaggggat gctttcgggg gcctgtccgg gcgaaatttt
     2401 cacctcaccg acgcccgata aaatctttga atgcgccatg caagttgatg gcggcgaagg
     2461 tgtactgttg attatcaaaa attacaccgg cgatattctt aactttgaaa cagcgaccga
     2521 gttactgcac gatagcggcg taaaagtgac cactgtggtc attgatgacg acgttgcggt
     2581 aaaagacagt ctttatactg ccgggcgacg cggcgttgcc aacaccgtat taattgaaaa
     2641 actcgtaggc gcagcggcgg agcgtggcga ctcactggac gcctgtgcgg aactggggcg
     2701 taagttgaat aatcaaggcc actcaatagg tatcgctctc ggtgcctgta ccgttcctgc
     2761 cgcgggcaaa ccttctttta ccctggcgga taatgagatg gagtttggcg tcggcattca
     2821 tggtgagccg ggtattgacc gccgctcctt ctcttccctt gatcaaaccg tcgatgaaat
     2881 gttcgacacc ctgctgggaa atggctcata ccatcgcact ttacgtttct gggattatca
     2941 acaaggcagt tggcaggaag aacaacaaac caaacaaccg ctccagtctg gcgatcgggt
     3001 gattgcgctg gttaacaatc ttggcgcaac tccgctttct gagctgtacg gcgtctataa
     3061 ccgcctgacc acacgttgcc agcaagcggg attgactatc gaacgtaatt taattggcgc
     3121 gtactgtacc tcactggata tgaccggttt ctcaatcacc ttactgaaag ttgatgacga
     3181 aacgctggca ctctgggacg ccccggtcca caccccggcc cttaactggg ataaattagg
     3241 agaaagcaat gtcactgagc agaactcaaa ttgttaactg gctcactcgt tgtggcgata
     3301 ttttcagcac cgagagcgag tatcttaccg gactggatcg cgaaattggc gacgctgacc
     3361 acgggctaaa tatgaaccga ggctttagca aagtggtgga aaaactccct gctatcgcag
     3421 ataaagatat cggtttcatt ctcaagaata ccggtatgac gctgctttcc agcgtcggtg
     3481 gtgccagtgg tccgctgttc ggtaccttct ttatccgcgc cgcacaggcg acccaggcac
     3541 ggcaaagcct gacactggaa gagctttatc agatgttccg cgatggcgcg gacggcgtaa
     3601 tcagtcgcgg gaaagccgaa cctggcgata aaaccatgtg tgatgtgtgg gtgccggtgg
     3661 tggaatcgtt acgtcagtcc agcgagcaaa atctctctgt tccggtggcg ctcgaagctg
     3721 ccagtagcat cgccgaatcc gctgcacaaa gtacgattac gatgcaagcc cgcaaaggcc
     3781 gcgccagtta tctcggtgaa cgcagtattg gtcaccagga tcccggcgcg acctcggtga
     3841 tgtttatgat gcaaatgttg gcgttagccg caaaagagta aggaattggt gatggtaaac
     3901 ctggtcatag tttcacatag cagccgactg ggagaaggtg tcggtgaatt agcccgtcag
     3961 atgttaatga gtgatagttg taaaatcgcc attgccgcgg gaattgacga tccacaaaat
     4021 cctattggta ccgatgccgt caaagtgatg gaggccatcg aatctgttgc tgatgccgac
     4081 catgtgctgg tcatgatgga tatgggtagc gcattattga gtgctgaaac tgcgctggaa
     4141 ttgctggctc ccaagatcgc cgcaaaagta cgtttgtgtg ctgcgccgtt ggtcgaaggt
     4201 acactggcag caacggtcag cgcggcctcg ggagcggata tcgacaaagt tatctttgac
     4261 gctatgcatg cgctggaagc caaacgtgaa caactgggtt taccgtcctc cgacactgaa
     4321 atctctgaca catgtcctgc gtacgatgaa gaagcccgtt ctctggcggt ggtcataaaa
     4381 aaccgtaacg gcctgcatgt acgtccggcc tcccggctgg tttatacctt atcgacattt
     4441 aatgccgata tgttgctgga aaaaaacggc aaatgcgtca caccagagag tattaaccag
     4501 attgcgttac tacaagttcg ctataacgat acgctgcgcc tgattgcgaa agggccagaa
     4561 gctgaagagg cactgatcgc tttccgtcag ctggctgaag ataactttgg tgaaacggag
     4621 gaagtcgctc cacctactct gcgtcccgtt ccgcctgttt cgggtaaagc cttttattat
     4681 caaccagttt tatatacggt acaggcaaaa tcaaccctga ccgtggaaga agaacaagat
     4741 cgattacgcc aggctattga cttcacgtta ttagatctga tgacgttaac agcgaaagca
     4801 gaagccagcg ggcttgacta tattgccgca atcttttctg gtcaccatac actgttagat
     4861 gatccggaac tgctggcggc ggcaagcgaa ctccttcagc atgaacattg cacggcagaa
     4921 tatgcctggc agcaagttct taaagaactt agccagcaat accagcaact ggatgatgaa
     4981 tatctacaag ctcgctatat tgatgtggac gatcttctgc atcgcaccct ggtccacctg
     5041 acccaaacga aagaagaact cccgcagttt aactcgccaa ctattctact ggcggagaac
     5101 atttatcctt ccacagtact gcaactggat ccggcggttg taaaaggtat ctgccttagc
     5161 gccggaagtc cggtatccca cagcgcccta atcgcccgtg aactggggat tggctggatt
     5221 tgccagcagg gtgagaaact gtatgcgata caaccagaag aaacgctaac gctggacgtt
     5281 aaaacgcaac gtttcaaccg tcagggttaa gccactgttc ggatggcatc gttctgatgt
     5341 catccggcaa tttactcagc actaatggtt tttaactacc tgttatattt ttcaccaatt
     5401 tatgatgatt tttcttcgct aatcacgaac ccaacgccgc tggctggcta ataaacagac
     5461 gttatttgat cactctattt cgccgttatt tatcccttta attccctttt ctaaaatgcc
     5521 tgacagttcg cagaatgaga tttcgatcat gcagctagtg cgatcctgaa ctaaggtttt
     5581 ctgatacttg aataccgttt tattcggttt cgccaaagga gaatgattga tgaaatcccc
     5641 cgcaccttct cgcccgcaaa aaatggcgt
//
LOCUS       DAJTOR010000217         5592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTOR010000217 DAJTOR010000000
VERSION     DAJTOR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.217"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..87)
                     /gene="dsbG"
                     /locus_tag="OO249_003801"
     CDS             complement(<1..87)
                     /gene="dsbG"
                     /locus_tag="OO249_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Involved in disulfide bond formation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5637926.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEK"
     gene            459..1022
                     /gene="ahpC"
                     /locus_tag="OO249_003802"
     CDS             459..1022
                     /gene="ahpC"
                     /locus_tag="OO249_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5637927.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1193..2758
                     /gene="ahpF"
                     /locus_tag="OO249_003803"
     CDS             1193..2758
                     /gene="ahpF"
                     /locus_tag="OO249_003803"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5637928.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2879..3307)
                     /gene="uspG"
                     /locus_tag="OO249_003804"
     CDS             complement(2879..3307)
                     /gene="uspG"
                     /locus_tag="OO249_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5637929.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLDRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            3528..4766
                     /locus_tag="OO249_003805"
     CDS             3528..4766
                     /locus_tag="OO249_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR5637930.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEEAQKAVSGLVNAMPGGTI"
     gene            complement(4997..5407)
                     /gene="rnk"
                     /locus_tag="OO249_003806"
     CDS             complement(4997..5407)
                     /gene="rnk"
                     /locus_tag="OO249_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5637931.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
BASE COUNT         1456 a         1332 c         1454 g         1350 t
ORIGIN      
        1 tttttcaatc gcttttactg gagcaggaag ttcctctgcg aaggcgattg caggaagcag
       61 agccagtaaa agtatctttt ttaacattaa tttgtccttt tcagtcagtg caaaagtcga
      121 gtaaaaggca taacctatca ctgtcatagg taagagctta gatcaggtga ttgccctttg
      181 tttatgaggg tgttgtaatc catgtcgttg ttgcatttgt aagggcaacg cctcagcctg
      241 caggcaggcg ctgaagatac caaagggtag ttcagattac acggtcacct ggaaaggggg
      301 ccattttact ttttatcgcc gctggcggtg caaagttcac aaagttgtct tacgaaggtt
      361 gtaaggtaaa acttatcgat ttgataatgg aaacgcatta gccgaatcgg caaaaattgg
      421 ttaccttaca tctcatcgaa aacacggagg aagtatagat gtccttgatt aacaccaaaa
      481 ttaaaccttt taaaaaccag gcattcaaaa acggcgaatt catcgaaatc accgaaaaag
      541 ataccgaagg ccgctggagc gtcttcttct tctacccggc tgactttact ttcgtatgcc
      601 cgaccgaact gggtgacgtt gctgaccact acgaagaact gcagaaactg ggcgtagacg
      661 tatacgcagt atctaccgat actcacttca cccacaaagc atggcacagc agctctgaaa
      721 ccatcgctaa aatcaaatat gcgatgatcg gcgacccgac tggcgccctg acccgtaact
      781 tcgacaacat gcgtgaagat gaaggtctgg ctgaccgtgc gaccttcgtt gttgacccgc
      841 agggtatcat ccaggcaatc gaagttaccg ctgaaggcat tggccgtgac gcgtctgacc
      901 tgctgcgtaa aatcaaagca gcacagtacg tagcttctca cccaggtgaa gtttgcccgg
      961 ctaaatggaa agaaggtgaa gcaactctgg ctccgtctct ggacctggtt ggtaaaatct
     1021 aaatttcctt cgtctttcgc gctaccaact ccggcgcgaa atacttcgga aattcaccta
     1081 attcttcggg tgctgcggca cccgattttc ttccccgcac tatgatgcaa gctgcatcca
     1141 ggtagccgta gaggccgctt gcatgatgat gtttaaagcc caggagataa acatgctcga
     1201 cacaaatatg aaaactcaac tcaaggctta ccttgagaaa ttgaccaagc ctgttgagtt
     1261 aattgccacg ctggatgaca gcgctaaatc ggcagaaatc aaggaactgt tggctgaaat
     1321 cgcagaactg tcagacaaag tcacctttaa agaagataac agcttgccgg tgcgtaagcc
     1381 gtctttcctg atcaccaacc caggttccaa ccaggggcca cgttttgcag gctccccgct
     1441 gggccacgag ttcacctcgc tggtactggc gttgctgtgg accggtggtc atccgtcgaa
     1501 agaagcgcag tctctgctgg agcagattcg ccatattgac ggtgattttg aattcgaaac
     1561 ctattactcg ctctcttgcc ataactgccc ggacgtggtg caggcgctga acctgatgag
     1621 cgtactgaac ccgcgcatca agcacactgc aattgacggc ggcaccttcc agaacgaaat
     1681 caccgatcgc aacgtgatgg gcgttccggc agtgttcgta aacgggaaag agtttggtca
     1741 gggccgcatg acgttgactg aaatcgttgc caaaattgat acgggcgcgg aaaaacgtgc
     1801 ggcagaagag ctgaacaagc gtgatgctta tgacgtatta atcgtcggtt ccggcccggc
     1861 gggtgcagcg gcagcaattt actccgcacg taaaggcatc cgtaccggtc tgatgggcga
     1921 acgttttggt ggtcagatcc tcgataccgt tgatatcgaa aactacattt ctgtaccgaa
     1981 gactgaaggg cagaagctgg caggcgcact gaaagttcac gttgatgaat acgacgttga
     2041 tgtgatcgac agccagagcg ccagcaaact gatcccagca gcagttgaag gtggtctgca
     2101 tcagattgaa acagcttctg gcgcggtact gaaagcacgc agcattatcg tggcgaccgg
     2161 tgcaaaatgg cgcaacatga acgttccggg cgaagatcag tatcgcacca aaggcgtgac
     2221 ctactgcccg cactgcgacg gcccgctgtt taaaggtaaa cgcgtagcgg ttatcggcgg
     2281 cggtaactcc ggcgtggaag cggcaattga cctggcgggt atcgttgagc acgtaacgct
     2341 gctggaattt gcgccagaaa tgaaagccga ccaggttctg caggacaaac tgcgcagcct
     2401 gaaaaacgtc gacattattc tgaatgcgca aaccacggaa gtgaaaggcg acggcagcaa
     2461 agtcgttggt ctggaatatc gagatcgtgt cagcggcgat attcacaaca tcgaactggc
     2521 cggtattttc gtgcagattg gtctgctgcc gaacaccaac tggctcgaag gcgcagtcga
     2581 acgtaaccgc atgggtgaga ttatcattga tgcgaaatgc gaaaccaacg tcaaaggcgt
     2641 attcgcagcg ggtgactgta cgacggttcc gtacaagcag atcatcatcg ccactggcga
     2701 aggtgccaaa gcctctctga gtgcttttga ctacctgatt cgcaccaaaa ctgcataaga
     2761 agaagtaaga ttcacctgca attgcttagc cgccggggtc aaacctagcg gcttttttat
     2821 ggcattaaaa agccctgccg ggatgttcca ggcagggcgg cataaatact tttatctgtt
     2881 agcgaacgac cagtacaggc agattggcgt ggcggatcac gctcgaggcg ttagaaccta
     2941 acagatgggt cgaaatcgat gggttgcgag aaccgataac tacaacatca gcccccagtt
     3001 cttctgccaa ctcattgact tcatcccgca cgctaccaaa acggacatgt tgtttaatgc
     3061 gggaaggatc gatggtgaag tggctgacca tcgtttgcag acgttcttct gcttcatgtt
     3121 gcagatgctc ttcaaaacga cgcacatcag cggcaaaacg gtccaggctc aggctggctg
     3181 acccgggtag tacgtgaagt agatgaataa ctccgtcatc ctgggtgagg aattcagcgt
     3241 ggcgaacagc tttgtcgctc aattccattt caaatacatc aactggcata atgattgtct
     3301 tatacataac cctttctccc tgttaatcat gaacaaatca ttcgccatga ttataatatt
     3361 tatccctgat atttgtctgg ttctttttcc ttacgaacgg tttctgtgat taatatattc
     3421 tcactgaaca ccaggaattc tcccaaaacc tgcggtaccg cccgttttcc cgctgtgata
     3481 gctaccctta aagactgact cttttttgaa ctgtctctgg aggttgcatg aaagcattga
     3541 cttatcacgg cccacatcac gttcaggtag aaaatgttcc cgatccgggc attgaacagg
     3601 cagatgatat tattctgcgt attacggcaa cggcgatctg tggctctgat ctccatcttt
     3661 atcgaggcaa aataccccag gttaaacatg gcgatatttt tggtcatgaa tttatggggg
     3721 aagtcgttga aaccggaaag gacgtaaaaa atttgcaaaa aggcgaccgg gtggtaattc
     3781 cgtttgtcat tgcttgtggc gactgttttt tctgtcgaat gcagcaatat gccgcctgcg
     3841 aaaataccaa tgcgggtaaa ggcgctgcgc tcaataaaaa acagatacca gctcccgcgg
     3901 cattgtttgg ttatagtcac ctgtatggtg gcgttcctgg tggacaggcg gaatatgtcc
     3961 gcgtccctaa agggaatgtg gggccgttta aagtaccgcc tttgctttca gatgataaag
     4021 cccttttcct ttctgatatt ctgccaacgg catggcaggc agcaaaaaat gcgcagatcc
     4081 aacaaggttc aagcgttgca gtctatggtg ccggtccagt gggattgttg acaatcgcct
     4141 gtgcacggtt gctcggtgcg gaacagattt ttgttgttga tcatcatccc taccgcttga
     4201 gtttcgccgc tgaccgctac ggcgcgatcc cgattaattt cgatgaagat agcgatccgg
     4261 cacagtcaat tattgaacaa acggcaggtc accggggcgt ggatgcagta atagacgccg
     4321 tcggttttga agcgaaaggc agcaccacgg aaacggtgct gactaacctg aaactggagg
     4381 gcagcagcgg taaagcgttg cgccagtgta ttgcggcggt caggcgtggc ggcattgtta
     4441 gcgtaccggg cgtctacgct ggatttattc acggtttcct gtttggtgac gcctttgata
     4501 aagggttgac gtttaaaatg ggacagaccc acgttcacgc atggctggga gaattattac
     4561 cgttaattga gaaaggatta ctgaaaccag aagaaattgt tacccactat atgccgtttg
     4621 aagaggccgc ccggggatat gagattttcg aaaaacgtga agaggagtgc cgtaaggtga
     4681 ttctggtacc cggtgcacaa agcgcagagg aggcgcagaa ggcagtttca ggtctggtga
     4741 atgcgatgcc ggggggaaca atatgatcgt caggagtggt tttcgaggta aaggacagcc
     4801 atgacgataa tcgccgccat aatcagaaat cctatcagga tgtaaaatgc ttctgccatg
     4861 gttattccca caaacgaaac ggaataattt tgcagcaaaa gtgaacagtg agaaccagga
     4921 aaaattgctg attttgcgta aagaggatgc gagtgcatcc tctgggcaaa gcgagttatc
     4981 gcttgtgctg atgggattaa agcaggtagt cgccagcagc ttctggctgg tattcgagtt
     5041 ccagcacttc aaggtgggtt gcaacgccgc ccggaagttc ccagtgaatg gaatcgccaa
     5101 cgcgcagccc cagcagtgcg gcacctaccg gagccataac ggaaagctga gtattgctat
     5161 cggtcatttt tgccggatac accagcgtgc gcacacgcac ttcgccatcg ctaagattgc
     5221 ggaatttaac ccggctgttc attgtcacca cgtcgtgtgg catctcttct ggcgaacaca
     5281 tttgggcgcg atccaactct gcgtttaacg cgtcggcgat tggcaaacca gcataggcgg
     5341 gttgctccag cagaatatcg atgcgttcgg catccaggtc gttaatgatg atagttggtc
     5401 tggacatttt tactccatgt cgtcggtact gcgagtgtcg cagataaaca tacccaaaag
     5461 aaaaccctca ccgtcaggcg gcgagggttt aactcacatg atgatactga ctgttgctca
     5521 ctctttgaag tgatttgcgt cacattcagg gaattcctca atgcagcgca ttatgtataa
     5581 ttcttaatcg tc
//
LOCUS       DAJTOR010000218         5583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTOR010000218 DAJTOR010000000
VERSION     DAJTOR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.218"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            28..243
                     /locus_tag="OO249_003807"
     CDS             28..243
                     /locus_tag="OO249_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5637932.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            236..622
                     /locus_tag="OO249_003808"
     CDS             236..622
                     /locus_tag="OO249_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5637933.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            630..1316
                     /locus_tag="OO249_003809"
     CDS             630..1316
                     /locus_tag="OO249_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5637934.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="OO249_003810"
     CDS             complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="OO249_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5637935.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1999..3204
                     /gene="tet(B)"
                     /locus_tag="OO249_003811"
     CDS             1999..3204
                     /gene="tet(B)"
                     /locus_tag="OO249_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5637936.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="OO249_003812"
     CDS             complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="OO249_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5637937.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4043..4414
                     /locus_tag="OO249_003813"
     CDS             4043..4414
                     /locus_tag="OO249_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5637938.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELKLTKKTILEIALQYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4424..>5583)
                     /locus_tag="OO249_003814"
     CDS             complement(4424..>5583)
                     /locus_tag="OO249_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5637939.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1542 a         1031 c         1214 g         1796 t
ORIGIN      
        1 ccactattaa gtcatgtagc tggttttatt tcaattgaac gttttcaaag tctagctaca
       61 gaaggaaaaa tgttatcgct atcttggtgg gaaaacgaat acgcagttct gcaatggaaa
      121 aatcatgttt tacatgcgaa agctcaacaa gaagggcgag agtcaatatt tgatttttac
      181 aaaattagta ttgctcatat tactcgcgaa tattcattta aaaaggacaa ggataatgtt
      241 tgatgttcac gttgttttag ataatcaaat aggacaatta gcattactag gaaaaacatt
      301 aggtaataaa ggtattggat tggaaggggg agggatattt acggttggtg atgaatgcca
      361 tgctcatttt cttgttgaac aaggaaagga agctaaaata gcgctagagc aagctggact
      421 gttagtactt gcgatccgga caccattaat tcgtaagtta aaacaggaaa aaccggggga
      481 acttggcgaa atagcacgag tattggcgga gaataacatt aatattttag tgcaatacag
      541 tgaccatgct aaccaactga tattaataac ggacaatgat agtatggctg catctgttac
      601 gctcccttgg gcaataaagt gaacttgcga tggctaattt aatacgaaaa gaggttacct
      661 ttgagtcctc aatagccgcg ataggggcgg ctatgtctga catttcacga gttaaaatac
      721 tcagtgcttt gatggatggg cgagcttgga cggccactga gctaagttct gtggcgaata
      781 tatcagcttc aacggcgagc agtcatttat ctaaattatt agattgccag ctaatcacag
      841 tagtagctca aggcaagcat cgttattttc ggctagcagg aaaagatatt gctgaattga
      901 tggaaagtat gatggggatc tccttaaacc atggcgtaca tgccaaagtt tccacgccag
      961 tgcatttacg aaaagcacgt acttgctatg atcatttagc tggcgaagtt gccgttaaga
     1021 tctatgattc cctttgtcaa cagcaatgga tcactgaaaa tggttcaatg atcacattaa
     1081 gtggtattca atattttcat gaaatgggaa ttgacgttcc ttccaaacat tcacgtaaaa
     1141 tctgttgtgc gtgtttagat tggagtgaac gccgtttcca tttaggtggg tacgttggag
     1201 ccgcattatt ttcgctttat gaatctaaag ggtggttaac tcgacatctt ggttaccgtg
     1261 aagttaccat cacggaaaaa ggttatgctg cttttaagac ccactttcac atttaagttg
     1321 tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg
     1381 gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt
     1441 ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa
     1501 atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa
     1561 aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg
     1621 tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg
     1681 taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa
     1741 catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt
     1801 aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac
     1861 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatcat
     1921 taattcctaa tttttgttga cactctatca ttgatagagt tattttacca ctccctatca
     1981 gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat tacgttactc
     2041 gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg tgaatttatt
     2101 gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc gttaatgcag
     2161 gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg
     2221 ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc aagtgcgctt
     2281 tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg ggctgtcgcg
     2341 gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt cggttggtta
     2401 ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt tgcaggagag
     2461 atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc tttccttgtg
     2521 gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac cgaagtaggg
     2581 gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc cattttgttg
     2641 attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg ggtgctattt
     2701 accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc gggtcttggt
     2761 cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa atggggcgaa
     2821 aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt tttagcgttt
     2881 atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg tgggatcgct
     2941 ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca aggtgcttta
     3001 cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt actgtttgct
     3061 gttatttata atcattcact accaatttgg gatggctgga tttggattat tggtttagcg
     3121 ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca agctcagggg
     3181 agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata
     3241 taatgaccct cttgataacc caagagggca ttttttacga taaagaagat ttagcttcaa
     3301 ataaaaccta tctattttat ttatctttca agctcaataa aaagccgcgg taaatagcaa
     3361 taaattggcc ttttttatcg gcaagctctt ttaggttttt cgcatgtatt gcgatatgca
     3421 taaaccagcc attgagtaag tttttaagca catcatcatc ataagcttta agttggttct
     3481 cttggatcaa tttgctgaca atggcgttta ccttaccagt aatgtattca aggctaattt
     3541 tttcaagttc attccaacca atgataggca tcacttcttg gatagggata aggtttttat
     3601 tattatcaat aatataatca agataatgtt caaatatact ttctaaggca gaccaaccat
     3661 ttgttaaatc agtttttgtt gtgatgtagg catcaatcat aattaattgc tgcttataac
     3721 aggcactgag taattgtttt ttatttttaa agtgatgata aaaggcacct ttggtcacca
     3781 acgcttttcc cgagatctca tctattgaaa cagcttgata gcctttttca acaaacaata
     3841 ttcgtgctga gttaaccagt gattgatagg tactcttaaa attttcttgt tgatgatttt
     3901 tattttccat gatagattta aaataacata ccgtcagtat gtttatggta tcatgatgat
     3961 gtggtcgtga caatcttaag aacatttagg ttattttatg tatattgaac agcattctcg
     4021 ctatcaaaat aaagctaata acatccaatt aagatatgat gataagcagt ttcatacaac
     4081 ggttatcaaa gatgttctat tatggattga acataattta gatcagtctt tactgcttga
     4141 tgatgtggcg aataaagcgg gttataccaa gtggtatttt cagcggctgt tcaaaaaagt
     4201 aacaggggtc acactggcta gctatattcg tgctcgtcgt ttgacgaaag cggctgttga
     4261 gttgaagttg acgaaaaaaa ctatccttga gatcgcatta caatatcaat ttgattccca
     4321 acaatctttt acacgtcgat ttaagtacat ttttaaggtt acaccaagtt attatcggcg
     4381 taataaatta tgggaattgg aggcaatgca ctgagagatc ccctcataat ttccccaaag
     4441 cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc agccacgagt aagtcttccc
     4501 ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc taagcgaact gttgagagta
     4561 cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt ttctgagcat
     4621 gaacgcccgc aagccaacat gttagttgaa gcatcagggc gattagcagc atgatatcaa
     4681 aacgctctga gctgctcgtt cggctatggc gtaggcctag tccgtaggca ggacttttca
     4741 agtctcggaa ggtttcttca atctgcattc gcttcgaata gatattaaca agttgtttgg
     4801 gtgttcgaat ttcaacaggt aagttagttg ctagaaccca tggctccttt gccgacgctg
     4861 agtagatttt aggtgacggg tggtgacaat gagtccgtgt cgagcgctga ttttttcggc
     4921 ctttagagcg agatttatac aatagaattt ggcatgagat tggattgctt ttagtcagcc
     4981 tcttatagcc taaagtcttt gagtgactag atgacatatc atgtaagttg ctgataggtt
     5041 tccagttttc cgctcctagg tctgcatatt gtacttttcc tcttactcga cttaaccagt
     5101 accaacccag cttctcaacg gatttatacc atggcacttt aaagccagca tcactgacaa
     5161 tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat tggtcatgag
     5221 ctttctttga acattgctct gaaagcggga acgctttctc ataaagagta acagaacgac
     5281 cgtgtagtgc gactgaagct cgcaatacca taagtcgttt ttgctcacga atatcagacc
     5341 agtcaacaag tacaatgggc atcgtattgc ccgaacagat aaagctagca tgccaacggt
     5401 atacagcgag tcgctctttg tggaggtgac gattacctaa caatcggtcg attcgtttga
     5461 tgttatgttt tgttctcgct ttggttggca ggttacggcc aagttcggta agagtgagag
     5521 ttttacagtc aagtaatgcg tggcaagcca acgttaagct gttgagtcgt tttaagtgta
     5581 att
//
LOCUS       DAJTOR010000219         5557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTOR010000219 DAJTOR010000000
VERSION     DAJTOR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.220"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(107..3964)
                     /gene="sigA"
                     /locus_tag="OO249_003815"
     CDS             complement(107..3964)
                     /gene="sigA"
                     /locus_tag="OO249_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5637940.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEVGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4325..5329)
                     /locus_tag="OO249_003816"
                     /pseudo
     CDS             complement(4325..5329)
                     /locus_tag="OO249_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5326..5557
                     /locus_tag="OO249_003817"
                     /pseudo
     CDS             5326..5557
                     /locus_tag="OO249_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1386 a         1255 c         1239 g         1677 t
ORIGIN      
        1 cagaaaaaga aagctaataa tgagagcaag agcgatatag tctgtcacag ggtaatacat
       61 attctctccg gtagaagaag ggccgcaaac gcggcccggg ctgttatcag aaagagtaac
      121 ggaagttggc gttgatcgcg ttatccacgt tgtatttacc aaatgccgat ttctcaaact
      181 caagaccaaa gcgaagatta tcgcgaattt cggcgttgag accaacattc atgagaatac
      241 gaccgtcttt ttcacctttg atacgtttct caccggacgc atcacgcagt acggtttcac
      301 cgttggcaaa caggtcaaac tggtagccaa ggccggcgcg ggctgtgact ttccagtcct
      361 taccggagaa ggatttaccc acatcaacac cggtacgccc aatcagcgga ttaaagtcct
      421 tatccttcat ggtcacgctc attccgcgat cctgccagtc gaaccgttta cctgatacgg
      481 ccccataaac cagttctgcc tgcggctcaa tccaggagtc ttctgttaca tgatagcggt
      541 agcctgcttc agcacccaca tacaaggaat gagaactgta gtcttttgtt ccgagcccag
      601 caaaggtcgc cgagtactca ttatcatggt gaacatactt actaatcagg tcgatatagg
      661 caccagagtc aaatatagcg gaagcataca gacctgcccc gacggacttc gtcttgccac
      721 tgaatgcatt actgctggca tgactgtcgg tatacgtcat agtcagacca gtgaaaaggt
      781 caagtccatc cagctcatgt tttttatcca caccaatctg cacatgggtg tagttgtcac
      841 tgtatccacc agaagctgaa cctgctccgc tcatgatgcg ggcccatgca ccgacctcgc
      901 cattggtgtc acgcagatcc cccattcgtt tattcaggtt gttgacttct gtcaggaagg
      961 ctttgtaatt accggacata tacgattttg ctgcactgga agatgctgta ttttcagcca
     1021 ccttatatcc ggtcagggtc cacgtggtat gttcctgatc ctctttcgtg ctgatcacag
     1081 gagatatgtt gctgaaacct atggcctgag tactggcttt aaatatctct ttattgcttc
     1141 cttttggggc tgttatcagg tcaacgttta atcctgagtt attatttccc actttctgta
     1201 tatcgaccga cagactgtta ttgcttccgg ttaactgatt tttaacaatg agtttatccg
     1261 attgatttac agactggctc agatcagccc gcataatcac agcagtatta gtggcatcca
     1321 gtgtgtttac tgtcacagta tggaaacccg aattctctat gttactaaac aacaaaagac
     1381 tgtcgcgggt ttttaatgat tgcagggctg actgtccgtt tactctccat aaggtgttat
     1441 acatgctggc tgaggcagca ggagcatcaa tacctccggt ataagaagta tcaaagccac
     1501 ccagcaaacc tgtagccagc ggtgtgtgtg tggttttgtc caggttatct tctttatact
     1561 ggtcaacacc aaacagaata gatgcttttt tgtctgattt tatattacca ttgacccagg
     1621 cataattttg agcctttaac gtggcaccat cttctgtcat attatatcca tctgacatat
     1681 agattgccgg gctgtattta ttattttctg taggggtgcc tgttaatgtc agggttccgg
     1741 ccagttcaat agctttatct acatacattt ttccctgggc ggtaacagta gctcctttgt
     1801 tgataatcag gtctgtatta attaaggata atgcaccagt catagtaaca gttgatttat
     1861 caccaacagt tattttgctg ttttgagcac tcagactgcc gttgaaacta ctttcgcctg
     1921 ctgaatcccc ggagcgaagc tgttgtctga atccaaaacc ttcgccagta atgtttcttc
     1981 ctgagaacat gtcaatgtat gcagttttat ttcccagaac aatttgagag tttttagcct
     2041 ctatgtgtcc ttttacatta gcatcccggc ctatactcag ggttgcgttt tctaaattta
     2101 atttccggaa attaaattta cgactttccc agtcgggctg ggaaaaagag gctatctgat
     2161 tgttggactt atattccgta ttgtaacgtt tatttacagt gctttcctgg tcctttatgg
     2221 cagcagaata gtctttttga cataaaaaag gaattggaca gttgttattg ccttctttaa
     2281 atatggcatg ttctgtcgca tgtccttgta atgttaactg tgcattccgg acagagatat
     2341 cgtttttgat atcgactgag ccatcaaaga tcagattggc attattgtta tgttgctggg
     2401 tagtattgat aatatgattt atgcttatat tacctgatac attcccatgg tacatatatg
     2461 catcagtatt agtaagtgat aatgttgatt gcttcacgtt tgagttcgtt acggttgttc
     2521 ccgcatctgt tgctgcgatt ttctgaaatg actggtcata gccgtttaga tccagtgttc
     2581 cgccatttct ggtaaaatag atttcgccat taccgctttc gcttaaagcg tctttggcat
     2641 ttatttttac cgtaccttta cctccggcca tgtaaatttt attgaaggct ttttcagcac
     2701 taaggatgac agtcccatta cctattttaa ggttatttcc ctgtgctatt tttacatcca
     2761 gagtaccact acctatttta tgcagattat ctccggcaac gcccttaaca ttccattcaa
     2821 cggtggtatt attatcaata tctatcccgg cacctttata agacttatct tttccgtaca
     2881 ctttatatgt ctggttctta tcgaatataa gcccgccaat gccaagatcg gtatcctgct
     2941 tgagcgttaa ttcccctcct cctgagacag acaggtcctt atcttttata atcgatgata
     3001 actcttgttt tttaccatcc agctgcatag tgtttgtgaa attctttttt aaattgttta
     3061 ccagaatatt attaaataac gagtagtttg tccaggctat attggtacta ctgataaaac
     3121 caactgtact ggtaacacca tggaaaaccc atttttgttg ttctttatca tatagataag
     3181 aaccagaacc actatcgcca aaaattggtg agtttttaaa aggggttctg ttggtataaa
     3241 tgtaactctt gtttttccag tccagattaa aaagacttgc gcttaacaag tcgggattat
     3301 agttttgtcc aaacagatat tgtttgccgt ttaatatcgt tgaggttgtt ccggcacctg
     3361 ctctgaagcc aattatttgt tccttaccgt tgtagtttac gccatatctt ttcatcgcat
     3421 cttcgggaga aagtgagaag tcggcactct ccgtaacccc tgttgtttcc acgacgaatt
     3481 tattcagacg atgaaccgaa aagtcgccag atgatactct gttacttgct ttatatgctg
     3541 tctgatccca tgactgtgtt gttattgcat gatgctgtgt accgttatga gtcgcagtaa
     3601 cactgtacgc acctccaatt gatgttgttg cacctttgtt ggaaacagca gaaaaatcag
     3661 gtacaggtag ttctggaaaa tgaaatatct ttccatcttt catcattaat tgaacatttg
     3721 ttgcacccgg tctgaactct cctctgtttt gagcaaggtc aagataatcg cgggtccata
     3781 tattatttat atccagcatt gctgcaaatg ctgtttcacc ataacttcca tatattgagg
     3841 ataaaataat accgaggata actttctttc ttgatgtacc gacactaact ttccgggtca
     3901 gttcagaaac agcaactaat ccacctgtaa tatgactata tttcagtgaa taaattttat
     3961 tcatacgtga actccgatat ttctgagttg tggttatggg atagcattta attacaacgg
     4021 ggaactatca gtcatggtac gtaactgata agggtataat gataatcact gtcagttata
     4081 tttgtatgac agatgtaaca aaatgaaaaa aaagataagc acaagtgatt gattggtcag
     4141 atattgatca cctggtgttt gtattttgac ctttgcgatc aaaatttgat ctttgttgtt
     4201 tccggtcaat cctttacagg ttttctgtat tatccggggg agttaagtct gtcgatgtcc
     4261 gacagacttt tgatgatgga aacaacatgc agcgataatg tgcatgctgt tttgcaggtg
     4321 gaactcagaa ggtgaactgg aactgtgcga ccgcaccgta agtgttgtcc gtgccgatca
     4381 ggcctgcaac atcaaggtga accacatcaa acggtgagat ccccaccccg gccgtgaatg
     4441 cactgccgtc gttaccggcc agattctgac ggtaaccggc gcgcagctgt gcccatttcc
     4501 aggcattaaa ttctgcgcca atggcggcaa actgacggtt gctgtcggag gtgaaaccgc
     4561 tggccggggt cagatccaca tcaaatgcgg tggtgaacat cgcgttgtgc caggagacac
     4621 cggccgtcac ctgcgggcgg atcctgaagg tttccgtgat accgttcact tcttttgtct
     4681 caatactacg ggggatcagg ttctgtgcga ccagtcccag cgtccagttg tcatccaggt
     4741 tcgtactggc accgatgtcg gcgttgatgc catttttcgt gttgtggtag cggtcgcctt
     4801 tgaaggcgct gctgtcataa tttctgacca gcacgttgta gttgaacagg tcgacgcgct
     4861 ggaatttggg ggggaagccc agagaccatt tctgcccggc cgtttccagt tccgtagcca
     4921 gagcaatacc cacatcagtg atcagtgccg cgcgggcaaa ggcctgtgat gtcaggcggc
     4981 ttttgtccac ctcctgcccg gtgctgtttg ccacctcttc caggtaagtg aggtcagcat
     5041 cagagacttt accgtcgaca ctgacggtgc cgtgtgattt aaccatcagt gcggcagaga
     5101 ggcgatcacc gggaagggcc gccacagcag aaacgcccag ctgtgcggcg gcatgtgtgt
     5161 tacggaagtc ccggaggtgt tctttcaggc gtgcggcagc ctcaggaaca ccgtggttac
     5221 tttcgatggc gcggtcgaag gccttccact catcactgat ccggtcggcg ttgtcggtga
     5281 tattatccgg attggagagc tgcgcgccga cggacggaag caggatgctg ggctcatgcg
     5341 cgacgcaaaa tccacgatgt atatatcagt actaaaagcg cgacggcgga agaagctctg
     5401 aaactaatcg gtgaactgta cgccatagag cacgatatac gcgggttacc ggtgtctgaa
     5461 cgcctggcgg tcaggcaaat gcagagtaga ccgctcctga cttccctgta taagctgatg
     5521 caggagaaag aacacacgtt atcgaagaaa tgtcgtc
//
LOCUS       DAJTOR010000220         5430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTOR010000220 DAJTOR010000000
VERSION     DAJTOR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.222"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO249_003818"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO249_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5637941.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO249_003819"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO249_003819"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5637942.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO249_003820"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO249_003820"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5637943.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OO249_003821"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OO249_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5637944.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1343 a         1290 c         1475 g         1322 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagtggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 cgcggttaac gacggcaacc catgcaccac gttctgtata gtactcggca acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggttgcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgtg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 atgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggt ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tcccgctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tggtggtaca tcctggcgcg ggtaacctgg atggcacggt actgaatgcg
     1261 ttgcttcatt attatccgcc cattgccgat gtaccgcgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaaaggcg tgaaattact cgtgagtatg aagcggtggc aattggtcat
     1441 atgaccgctg gcggtacagt agatgaacca atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atcccatggg caaaccggcg gtcactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaaccggac gtacgcacca gatccgcgtg
     1621 catatggcgc atattaccca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcgtttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ctatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cagaagatat ggtggagttg attgaggtta tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tctacccgta tcggcggcgt gagcttgcca ccttacgact
     1981 ctctcaacct tggcgctcat tgtggtgata acccggagca cgtagaggag aatcgcaagc
     2041 aactttttgc tgcgggaaat ttgccttcaa aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagctcact ggcgaacctt atgcctcaaa acgggcggat gcctcttata
     2161 gcaatacgcc cggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgattcggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgaggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcagc aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaaa
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gacgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg agtttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaacat tctgttgcag gtactggatg atggtcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatttgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtaa
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
     5341 ctcgtttttg tctataagtt agacggaaaa gactatattt aagatgtttt gcctgaaaaa
     5401 tgagcgaacg ataaagtttt tatatttttc
//
LOCUS       DAJTOR010000221         5394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTOR010000221 DAJTOR010000000
VERSION     DAJTOR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.223"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1075
                     /locus_tag="OO249_003822"
     CDS             <1..1075
                     /locus_tag="OO249_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR5637945.1"
                     /translation="VEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETA
                     RLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPV
                     SLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELG
                     QQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPE
                     LFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQA
                     YPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYE
                     GVAMEYAFLVALAQGTLPATPAD"
     gene            complement(1072..1734)
                     /gene="exoX"
                     /locus_tag="OO249_003823"
     CDS             complement(1072..1734)
                     /gene="exoX"
                     /locus_tag="OO249_003823"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5637946.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWSFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(1758..2414)
                     /locus_tag="OO249_003824"
     CDS             complement(1758..2414)
                     /locus_tag="OO249_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR5637947.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLACDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(2516..2746)
                     /gene="holE"
                     /locus_tag="OO249_003825"
     CDS             complement(2516..2746)
                     /gene="holE"
                     /locus_tag="OO249_003825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5637948.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            2885..3259
                     /gene="yobA"
                     /locus_tag="OO249_003826"
     CDS             2885..3259
                     /gene="yobA"
                     /locus_tag="OO249_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5637949.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3263..4135
                     /gene="copD"
                     /locus_tag="OO249_003827"
                     /pseudo
     CDS             3263..4135
                     /gene="copD"
                     /locus_tag="OO249_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            4148..4489
                     /locus_tag="OO249_003828"
     CDS             4148..4489
                     /locus_tag="OO249_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5637950.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(4871..>5394)
                     /locus_tag="OO249_003829"
     CDS             complement(4871..>5394)
                     /locus_tag="OO249_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5637951.1"
                     /translation="NCIREAADQLPAWFPLAMDLALVTGQRREDITNMRFSDIYDDRL
                     HIRQIKTGMMIAIPLSLSLPVAGLRLGAVVEQCRMVSRGDYLISAGIRKNSPDGSIHP
                     DGLTKKFVAARKLTGIQFSENPPTFHEVRSLAGRLYKETCGEEFAQRLLGHTSEKTTK
                     MYLDEREKTYLLL"
BASE COUNT         1336 a         1282 c         1347 g         1429 t
ORIGIN      
        1 ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa attaaccgca agacccggga
       61 agtcattggt attgcctttg atgatccagc ctatgtgacc tggattgcct acaatccaga
      121 acctgaaacc gcgcgattgc gttatggtta ttcttccatg accacaccag acactttgtt
      181 tgaactggat atggataccg gtgagcgtcg tgtattaaaa caaacggaag ttcctggttt
      241 tgatgcggcg aattaccgca gtgaacacct gtggatagtc gcccgtgatg gcgtcgaagt
      301 tccggtttcg ttggtctacc atcgcaaaca ttttcgcaaa ggacacaacc cgttgctggt
      361 gtatggctat ggttcttacg gcgcaagtat tgatgccgat ttcagtttta gccgcttgag
      421 tttgttagat cgtggctttg tctacgccat tgtccatgtt cgcggcggtg gtgagctggg
      481 gcaacaatgg tacgaagacg gaaaatttct gaagaagaaa aatacgttta atgattatct
      541 tgatgcctgc gatgcattgt taaaactggg ctatggctct ccttcgcttt gttatgcgat
      601 gggcgggagt gcggggggca tgttgatggg cgttgcaatt aatcaacgcc cggaattatt
      661 ccacggcgtt atcgcccagg taccgtttgt tgatgttgta acaacgatgc ttgatgaatc
      721 aattcctctt accactggtg agtttgaaga gtggggtaac ccgcaggatc cgcaatatta
      781 cgagtacatg aaaagctaca gcccgtatga caacgtcacc gcacaggctt atccgcattt
      841 actggtaacg accggtttac acgattctca ggtgcaatat tgggaaccgg caaaatgggt
      901 cgctaaattg cgcgagctga aaaccgataa ccatctttta ttgctctgta ccgacatgga
      961 ctcaggccat ggcggtaaat ctggccgctt taaatcgtac gaaggcgtag cgatggaata
     1021 tgcttttctg gtcgcgctgg cgcagggaac attacccgct acgcctgcgg attaagtatt
     1081 ttccagataa tgtttcagtg ttaaacgcag ctccgggctc atgctgtcca ggttattaaa
     1141 tgaccagcgc agatagcccg gatcgcgttc ggcaacgtcg gaaaccgctt tgccacggta
     1201 tttgccaaag gtgaaggtcg tcatcaacga cggacgtccg gtgatatcgg ccatctgttc
     1261 tgccgtccag ccggaggtgt tcataatatc gataagcaac gcggcggtga tataacagtc
     1321 atacaacgcg cggtgatgat gcaggcccgg cggcgtctgt acattgagct tccgtgtttt
     1381 atataacgcc atattgctgt acttgatccc tggccacaaa cgacgggcca gtttcatagt
     1441 gcaaatccac tcaccgggca tctcaggcag tacgcggcgg tcaaagctgg cgttgtgcgc
     1501 gacataccat tcactaccgt aatagtgtgg gatcacatct tcaatccacg gtttatcggc
     1561 gaccatggct tcggtgatgc gatgaatcgc catcgcttgt ggactaatag gacgatcggg
     1621 gcgcaccagg tggctcatgg ggttgacgat ttttccgtca atgacatcaa cagaggcaat
     1681 ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg ataatgcgca acatggcatg
     1741 ctccaggccg aaaaagccta gcgtaatgga atgatatcgc cttgccaacc ctgtgatgaa
     1801 cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa cgatgagacg accatattca
     1861 tcccatagtg cactgttacc acgggcattt gccatcagca cggcaatcga gtactgatgt
     1921 gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg gttctcccag acattgccct
     1981 gtagtaaaca gtgaacaggt cgggtccata tccatacctt gcggttgttc atcgaccacc
     2041 gtgatggttc tggaacgacg gcccagacat gcaccatgac tctgatggta gatcccaggc
     2101 gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa agcgatcgtt atattcaacg
     2161 ggaaggccgg caatgatggt cattcgccag gtcgttgcgg cataacaaag cgggtctaac
     2221 agtgagagat cgggtggggc aggaagggca cgtcgcgaat aatcacacgc cagtaaagaa
     2281 agcgatggaa aaactaacag ctgacattgt tgcctggcgg cggccctcac aaactccaga
     2341 tgatgagcta cctgttcggt gagtgaggtt ttacgaggct catattgcgc tgctgcaact
     2401 ttccagaacg acataatgac ttccttttca aaatgactgc aaattatggg tgcggtttag
     2461 aaaatactca taatttagca ggcgttatgt aagaaagtgt aactctgtat aagttttatt
     2521 taagtttggg ctcgtaaggt aaacgtgaca gtttgaccga agccaaacgg tgggcaataa
     2581 gcctctcgcg aaaccagctg cgcaaatgtt caggctgttc acgttcaacc gcttcagcga
     2641 tcaccggcat attgtagcgt tctttaaatg ccaccccggc cgccgccaaa tcgacattca
     2701 ctttatccat ttctgtttga tccagtttag ccagattctt cagcattctt aatctcctca
     2761 tcattcgcgg gcaaaagtta ctaagagtga gccaggatgg caagccggaa ttgatgatcc
     2821 tcgactcaga cattagacaa ggttatgctc tgtaaaaaag ttattacaaa aagtaataga
     2881 taatatggct tcaactgcac gctcccttcg ctacgcgctg gcaatcctga ccacttcgtt
     2941 ggtgacacct tccgtctggg cacatgcgca tttaacgcat cagtatcctg cggcaaacgc
     3001 gcaagtgaca gctgcaccgc aggcaatcac tttaaacttc tcggaaggtg ttgaaacagg
     3061 attcagtggt gcaaaaatca cggggccaaa aaacgaaaat attaaaacat taccggcgaa
     3121 gcgaaatgag caggaccaaa agcaactgat tgttccactg gccgattcgc tgaaacccgg
     3181 aacctacacc gttgactggc atgttgtttc ggtggacggg cataaaacga aaggacacta
     3241 cacctttagc gtgaaataaa tcatgctggc gtttacctag atcgcgctgc gatttatcca
     3301 tttcacctcg ctgatgctgg tttttggctt cgcaatgtac ggtgcctggc tggccccctt
     3361 gacgattcgt cgcttgctgg cgaagcgttt tctgcgttta caacagcacg ctgccgtctg
     3421 gagtttgatc agtgccactg caatgctcgc cgttcagggc gggttgatgg gaacgggatg
     3481 gacagatgta ttttcgccaa acatttggca ggctgttttg caaacgcagt ttggtggcgt
     3541 ctggttatgg caaattgttc tcgccctcgt aacgttgatc gtcgccctta tgcaaccgcg
     3601 gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa tttattctgc tggcgggagt
     3661 ggggcatgcg acgctgaatg aaggggtaac ggcgaaaatc catcagacta atcacacgat
     3721 tcacctgatt tgtgcggcgg cgtggtttgg gggattactg ccggtgctct ggtgtatgca
     3781 actcatcaaa ggccgttggc gacaccaggc tattcaggcg ctgatgcgtt tttcatggtg
     3841 cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg cttagtgcat tgttgattac
     3901 cggatttccc cccacgctta cgacttactg gggccaactc ttattgctca aagccatcct
     3961 ggtgatgatt atggtggtca ttgcgctggc taatcgttat gttcttgtac cgcgcatgag
     4021 gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg actaaactcg aatgggcgat
     4081 aggtgcggta gtgctggtga tcatcagcct gcttgcgact ctcgaacctt tttgatggat
     4141 tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta ctcaccagtt ctgcagcggc
     4201 gctggctgca ccgcaggtga ttaccgtcag tcgttttgaa gtgggtaaag acaaatgggc
     4261 gtttaatcgc gaagaggtga tgctgacttg ccgaccgggt aatgctttgt atgttattaa
     4321 tccgagtacc ctcgtgcagt atcctttaaa cgatatcgca caaaaggaag ttgccagtgg
     4381 gaagaccaat gcccaaccca tttcggtgat tcagattgat gatcctaaca atcccggcga
     4441 aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa ctctgttaat tacctaaaat
     4501 agccttttga tttccaataa aaaaaccgcc tcagttcttt caccagaacg ggcggtttta
     4561 acatttcagc tgatgaccac cacgcttttt attgaccatt ttgcacgcaa actggaaaac
     4621 ctggcgtcgt catctattct taaagggcaa ggcaactaag cctgcattaa tgccaacttt
     4681 tagcgcacgg ctctctccca agagccattt ccctggaccg aatacaggaa tcgtattcgg
     4741 tcttttttta attgcatttg aaatcaatta gttgcaaatg tctccccgaa atttactcga
     4801 atttctgtat tccggtcttt tttggttata tcacaaccaa aatacattta acaatccatt
     4861 tacgttaaaa tcagagcagt aagtacgttt tttctctctc atcaagatac atttttgttg
     4921 tcttctccga tgtgtggcca agtagacgct gagcaaattc ttctccacat gtttctttgt
     4981 acaatcgtcc agccaggctt ctgacttcgt gaaaagttgg tgggttttca ctgaactgga
     5041 tacctgttaa ttttctggct gcgacaaatt tctttgtcag gccgtccggg tgaatgctgc
     5101 cgtcagggct gttttttcta atcccggcac tgattagata atccccccta cttaccatgc
     5161 ggcactgttc aactactgca ccaagtcgta aaccagcgac aggaaggctg agtgacaggg
     5221 ggatagcaat catcattcct gtcttaattt gcctgatgtg gagacgatca tcataaatat
     5281 cactgaaccg catattcgtt atgtcttcgc gacgttgtcc tgttacaagg gctaaatcca
     5341 tagctaatgg gaaccatgcc ggaagttgat ctgctgcctc cctgatgcag ttgt
//
LOCUS       DAJTOR010000222         5344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTOR010000222 DAJTOR010000000
VERSION     DAJTOR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.224"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..904
                     /gene="adeQ"
                     /locus_tag="OO249_003830"
                     /gene_synonym="yicO"
     CDS             <1..904
                     /gene="adeQ"
                     /locus_tag="OO249_003830"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5637952.1"
                     /translation="LRIGITSGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLL
                     GILGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDL
                     SGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSV
                     AGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGA
                     LIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTG
                     RWRDLNLCVVVVAALFALKIILVD"
     gene            957..1409
                     /locus_tag="OO249_003831"
     CDS             957..1409
                     /locus_tag="OO249_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5637953.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRISDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1620..2810
                     /gene="nepI"
                     /locus_tag="OO249_003832"
     CDS             1620..2810
                     /gene="nepI"
                     /locus_tag="OO249_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5637954.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRCYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(2851..3144)
                     /locus_tag="OO249_003833"
     CDS             complement(2851..3144)
                     /locus_tag="OO249_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637955.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3366..4184
                     /gene="nlpA"
                     /locus_tag="OO249_003834"
     CDS             3366..4184
                     /gene="nlpA"
                     /locus_tag="OO249_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR5637956.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLHTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4211..5110)
                     /locus_tag="OO249_003835"
     CDS             complement(4211..5110)
                     /locus_tag="OO249_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5637957.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRPEK"
     gene            complement(5221..5316)
                     /locus_tag="OO249_003836"
     CDS             complement(5221..5316)
                     /locus_tag="OO249_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR5637958.1"
                     /translation="MVEIWSYHALFWLAIGMLGIAMICLLFIKDI"
BASE COUNT         1273 a         1216 c         1373 g         1482 t
ORIGIN      
        1 tttacgtatt ggtatcacca gcggaattgg attatttatc gccttaatgg gattaaaaaa
       61 tactggcgtt attgtcgcca ataaagacac gctggtgatg attggcgatt taagttctca
      121 cggcgtgttg ttaggtattt tagggttttt tattataacc gtgttgtcat cacgtcattt
      181 tcatgccgcg gtgctggttt ctatagtggt gacgtcttgc tgtggattat ttttcggtga
      241 tgttcatttt agcggcgtct attccattcc gcctgatatt agcggcgtca ttggtgaagt
      301 agatttgagc ggcgcgttaa cacttgaact cgccggtatc attttctcct ttatgctgat
      361 caacctattt gattcatcag gaacattaat tggtgtaact gataaagcgg gcttaataga
      421 tggtaacggt aaattcccca atatgaataa ggcgctgtat gttgatagcg tcagttcggt
      481 ggcgggtgcg tttatcggca cctcgtctgt tactgcctat attgaaagta cttctggtgt
      541 ggcagtcggt ggccgcacgg ggctgactgc ggttgtggtt ggcgttatgt tcctgttggt
      601 tatgttcttc tcaccgctgg tggcgatggt tcctccttac gcaaccgccg gagcgttaat
      661 ctttgttggc gtgctgatga cttcgagcct ggcgcgcgtt aactgggatg attttaccga
      721 atcggtgcct gcgtttatta ccacggtgat gatgcccttt actttctcga tcaccgaagg
      781 gattgcactc ggctttatgt cgtactgcat catgaaagtg tgcaccgggc gctggcgcga
      841 tctgaacctg tgtgtggtgg tggtcgcagc tctgtttgca ctgaagatta ttctggtgga
      901 ttagtaactg gcgagggttt ccagatatca tgagttctga ttacgcagga gaactcatga
      961 tctggataat gctcgccacg ctggcggtag tgtttgtggt tggttttcgg gtgctgacat
     1021 ccggggccag aaaagcgatt cgccgtatca gtgatcggct gaatatcgat gtcgtacccg
     1081 tggagtcgat ggtcgatcaa atgggaaagt cagccggtga cgaattttta cgttatttgc
     1141 atcgcccgga tgagtcgcac ctgcaaaacg ccgcgcaggt gttgctcatc tggcaaattg
     1201 tcattgtcga tggtagcgaa cagaacctgc tgcaatggca tcggatttta caaaaagccc
     1261 gtcttgccgc gccgattacc gatgctcaag tcaggctggc gctgggtttt ctgcgcgaaa
     1321 ccgaacctga aatgcaggat attaatgctt ttcagatgcg ctataacgcg ttctttcagc
     1381 ctgccgaggg cgttcactgg ctgcattaaa accgcttcgt cttatcagcc tacagaccgc
     1441 gcctgacctg tcgggtggat aagacgttca cgccgcatcc ggtaatcttt gccctgttcc
     1501 gtttcgatct taactccagc tcaagcaaca ccgtaaaagt tgcaaaaacg ttaaatgcat
     1561 cacacatttc aatgttaaag tggtcggctt ttcccctgaa acatgccacg ggtaacacca
     1621 tgagtgaatt tattgccgaa aaccgcggcg cggatgccat cacccgaccg aactggtcag
     1681 ccgttttctc ggtggcgttt tgtgtcgcct gtctgattat cgttgagttt ttgcccgtca
     1741 gtttgttgac gccaatggcc caggatttag gcatttcgga aggggttgcc gggcaatcgg
     1801 tgaccgtgac cgcctttgtg gcaatgtttg ccagtttgtt tattacccag acaattcagg
     1861 ctactgaccg ctgctacgtt gttattttgt ttgccgtttt gctgacgctc tcctgcttgc
     1921 tggtttcctt tgctaactca ttcagtttgc ttttaatcgg tcgtgcctgt ctggggctgg
     1981 cgctgggcgg gttctgggcg atgtcggcgt cgctgaccat gcgtctggtg ccgccgcgta
     2041 cggtgccgaa ggcgctgtcg gtgatcttcg gcgcggtttc tattgcgctg gtgattgccg
     2101 cgccgttggg cagtttttta ggcgagctta tcggttggcg caatgtcttt aatgcggcgg
     2161 cggtgatggg cgtgctgtgt attttctgga ttatcaaatc attgccttca ctgccaggcg
     2221 aaccctcgca tcagaaacaa aatactttcc gcttattaca acgtccgggt gtgatggcag
     2281 ggatgatcgc catcttcatg tctttcgccg ggcagtttgc tttcttcacg tatattcgcc
     2341 cggtgtatat gaacctggcg ggattcggcg tggatggctt aacgctggtg ctgttgagtt
     2401 ttggtatcgc cagctttatt ggtacgtcgc tttcgtcgtt cattcttaaa cgttcggtaa
     2461 aactggcctt agcaggcgcg ccgttaatac tggctgtgag tgcgttggta ctgacgttgt
     2521 ggggaagcga taaaatcgtt gctaccggcg tggcgattat ctgggggcta acttttgcat
     2581 tggttcccgt cggctggtca acgtggatca cccgctcgct ggccgatcag gcagaaaaag
     2641 ccgggtctat tcaggtggcg gttattcagc ttgctaatac ctgtggcgcg gcaatcggcg
     2701 gttatgcgct ggataatatt ggtctgactt cgccgttgat gttgtccggc acattgatgt
     2761 tgctgactgc attgttggtt actgcaaagg tgaaaatgaa gaaatcctga tatcaggtaa
     2821 agaagcgttg gtcagtagtt gttgctgttc ttacatatcc gggcattcta ccttacccaa
     2881 cgcttcccaa taattgctct gattattacg ttccatcgcc acgatggcct cgcgtatatg
     2941 ttgtttattg ttttcatctg acaacattaa cttttcccac gcttcatctg ataatgacgc
     3001 ctgtggcgtg caaattttac gtaaatcttg taataccgtg gttttctctt ttgcggactg
     3061 cagtgagcta aaaggcgact cggcgtatac gatgcctggc atagcgaaaa aagtgaaaat
     3121 aagcagtagt gtcgttggtt tcatggattc aaccttttac aaatgtgtat cgaatgaagc
     3181 ataagtgtag tttgctttca tcgctctcat cattctgtac gaaacgcgct tgcgtggtca
     3241 gtaagaagtg ccagacttta tattccactt ttattccttt ttattcttat cgatagcgtt
     3301 tcgtttttta aaccgcagcg accttaccgc tatagtcagg taatcattaa taaaaggata
     3361 aaaaaatgaa actgacaaca catcatctac ggacaggggc cgcattattg ctggccggaa
     3421 ttctgctggc aggttgcgac cagagtagca gcgatgcaaa acacattaaa gttggcgtta
     3481 taaatggcgc agaacaagat gtcgcggaag tcgctaaaaa ggtggcaaaa gagaaatatg
     3541 gcctcgatgt tgaactggta ggatttagcg gttcgttgct accgaacgat gcgacaaatc
     3601 atggcgaact tgatgcgaat gtcttccagc atcgaccctt tcttgaacaa gataatcagg
     3661 cgcatggtta taaactggta gcggtgggaa atacgtttgt cttccctatg gcgggttatt
     3721 ccaaaaaaat cacaaccgtg gcgcaaataa aagaaggcgc gacagtggcg atccccaacg
     3781 acccgaccaa ccttggtcgc gcacttttac tgttgcaaaa agagaagcta attactctga
     3841 aagagggaaa agggttatta cataccgcgc tggatataac cgataatcct cgtcatttgc
     3901 agattatgga actcgagggg gcgcagcttc cgcgcgtact ggatgatccc aaagttgatg
     3961 tagcgattat cagcaccact tacattcagc agacagggct ttctccggtg cacgacagcg
     4021 tatttattga agataagaat tcgccgtatg tgaatatttt ggtggcacgg gaagataata
     4081 agaatgcaga aaacgtgaag gaatttctgc aatcttatca atcacccgaa gtcgctaaag
     4141 cggcagaaac catttttaac ggtggcgcgg tgcctggctg gtaattctca ctcatgccgc
     4201 gccggacgat ttatttttct ggccggcgac gggaatccat tgaaatcaaa atcaccgatg
     4261 acagaatcag cagcgttccc agccagtccg gtaatgtgaa cgtgatcccc agcaacagca
     4321 aagagagtag cgcgctactt aatggttctg cacagctcaa aatgctggct tttggaccgc
     4381 caattaattg tgctcctttc aggtacagac taaatgtcag ggacgtacca atgaccacca
     4441 aataaaaaaa cgccagaatc aaactgccgt taacgacaaa gtttgttcct tgtctggcat
     4501 aaaaaggcaa cagaatcaga ccgccaatca gcatactcca gccgacgact ggtaacgtgc
     4561 catagcgggc aattagcgtt gagggatagg tggtatagaa tgcagcagca aatgccgagg
     4621 caatgcccca gaacaacgcg gcaggagaga gcgataatga cgtcggatta ccgtgtgtca
     4681 ccaataaaaa agtcccgacc agcgatgtca aaatagcgca gaaaaccaga atgcccgggc
     4741 gcgatttacg caccagtgag aaccaggcga cgataatcgt cggtgagagg aattgcagca
     4801 ccgttgccgt ggctgcgttc gatttttcga tggttagcaa aaaagtgagc tgtacagtta
     4861 gcgcgccaac cacggaaaaa atcagcaggc taatggcatc tttatgattg ttaataatag
     4921 aaaagatttt atcgccatga acaaatgaca gcgtcagtag aatcaaaccg gcgaatatca
     4981 aacgcgtcat agtcaaaaac tgcgacgaca tctggctttg ctccatgatg tattgcgcgc
     5041 agacccctga acttccccac aacacggcgg caatcagaac gttcagcatc ccctttctgg
     5101 tggaacccat ttttccctct gcatactgtt ttgtcgttta tgtatgcagg catgatagca
     5161 aaaatggcga ggatggctct gtttgcgcca cgccgatttt tcttagccca gactggtgag
     5221 ctaaatatct ttaataaaca gcaggcaaat catcgcaata cccaacatcc ctatcgccag
     5281 ccagaacaac gcatggtagc tccagatttc aaccataatt ccgtcaacag agccggcgat
     5341 aatc
//
LOCUS       DAJTOR010000223         5328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTOR010000223 DAJTOR010000000
VERSION     DAJTOR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.225"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(890..1831)
                     /gene="fabY"
                     /locus_tag="OO249_003837"
                     /gene_synonym="yiiD"
     CDS             complement(890..1831)
                     /gene="fabY"
                     /locus_tag="OO249_003837"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5637959.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1876..2313)
                     /gene="dtd"
                     /locus_tag="OO249_003838"
     CDS             complement(1876..2313)
                     /gene="dtd"
                     /locus_tag="OO249_003838"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5637960.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2310..3183)
                     /locus_tag="OO249_003839"
                     /pseudo
     CDS             complement(2310..3183)
                     /locus_tag="OO249_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
     gene            complement(3177..3776)
                     /gene="yihX"
                     /locus_tag="OO249_003840"
     CDS             complement(3177..3776)
                     /gene="yihX"
                     /locus_tag="OO249_003840"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5637961.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3917..4804)
                     /locus_tag="OO249_003841"
     CDS             complement(3917..4804)
                     /locus_tag="OO249_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5637962.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPAGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4794..>5328)
                     /locus_tag="OO249_003842"
     CDS             complement(4794..>5328)
                     /locus_tag="OO249_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5637963.1"
                     /translation="YISSQLVLSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLY
                     LSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVR
                     YTGLSFAYQFSAILGGLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTD
                     EQETASYRYIREQSHEN"
BASE COUNT         1435 a         1342 c         1254 g         1297 t
ORIGIN      
        1 tgataaatga agaaaaaccg tattcatcgc cattaccacc accttaccta tattactcat
       61 taatgtatgt gcgaaaatta cgccggaaaa tcattaaaaa aaagcggatt tttgcatgtt
      121 gttttgtttt tgcgaggcgt tttccagaaa taataagagt gttgcagttg tttcatttat
      181 tactcaaaaa tggtttagtt atcgcccagg atgaattatg ggtgctatga atatattata
      241 ttcagagata tttatttgca taatctgaat ggtgtagccg aaaaacgtca gcatcctaac
      301 agcaccagga tttaaggtga aattaatctt tcatcacaga ccggtcagta ctgccaccaa
      361 ccggtcttta cttgctcatc gttataattt tgttggtaac agttcgggaa agttgttttt
      421 ctaaattaac aacagaataa ctttcttgtt taatgagatt aatattggca accattatag
      481 cctccttccc acggtttaag agcaaacata caacataatg attcataaca tacagagcag
      541 taaatgaatg atactgacaa tgatgccagc ccattatctt tgcataatgt ttgtgagcta
      601 tagattttaa aaatatttat gaggaaagta tggcaccaga ggaggttttg actatcaaaa
      661 atcgctcaaa cctgaactaa gatgatgagt taagcattat ctgcgataat tgccactcag
      721 ctctacaaat aatatttaaa tgctcctgaa cagtgatgta gcaagacacg tatggtgcca
      781 acgaagaata aaaatttata gagttcttat tcaatattac cggcatagac agggaatgat
      841 gtaaatgacc ggaatgaatg agagaggcag caccgcccct ctccataagc tactcttctt
      901 cgttcccgcc ctcttcatac gggccaaatg gcttcgcggg cagaacgata tacgtgcctt
      961 caaacactgc acccggcgtc tcgtcgccaa agatttcaac ttgcatctgc acccgtgctt
     1021 ttcgtccgcg cgccagacgg tcgagatcgc cgcttaaggc accaaggtcg gctaccgcat
     1081 gaggtttacc gctaatcggc ttgctgtagc ggatatgcgc atcggccaga ataatcgttc
     1141 cgccgaggtg gcgttcgcgc agcatcagcc agataagccc ccaaccggtg agcgttgcca
     1201 gcgagaataa actcccggca aacagcgtat ggtgcggatt ctgattaccg gtttccggca
     1261 tggtggtgat aaatttttgc ccggtatatt gctgaatgcg cacgcccatt ttttcactaa
     1321 gcgggatgtg ttcgtaccac gcctgttgca gctgcgcgca ccagtcgccg cgatgcaaaa
     1381 tatcatccag agtggcaaca ggtttaatca tcaaaaaatg gcgaatcggc gtggtggttg
     1441 gtgtggtgat ttctccctga ttaacaaacc ccagcttggc gaaaaactcc accgcgtctt
     1501 cacgggcgct acaggtcacg cgcttaacgc cttcctgacg cgccaccgac tccagggtca
     1561 tcgccatcaa cgtccccagc cctttgtcct gcacgtcggg atgaacggcc ataaagcgaa
     1621 tggacgcttc attgtcggca ttaatataca gtcggcctac cgccaccaga ttcccctgct
     1681 cgtcgacgac catctgatga tgcgccatcg catcccacgc gtcgcgttcc gaaccttttg
     1741 gttgatgcag gggcttacgc aacatttccc agcgaaactg atagtaacgc tctaactctt
     1801 cttctgtttg tggaacccga aggtgataca tagcgatact ctctcttgtt tcccgtgaca
     1861 accctggaag ctggctcata cctgcaacca gaatgtcacg gggccatcat tgaccagcga
     1921 tacctgcata tcggcagcga agcgtcctgt ttgcgtgttc atctcttgct ggcggcagcg
     1981 ttcgacgaaa tagtcatata acgcctctgc gcgatccggt gatgcgcctt tggagaaact
     2041 tgggcgcatc ccccgttcgg tatctgcggc gagggtaaac tgggaaacca ccagtacact
     2101 gccgcccgcc tgttgcacgt tgagattcat cttgccttcg gcatcgctaa agatgcggta
     2161 gccgagcaca cgctcgcaca gacggtttgc tttctgttcg tcgtcatcct tttcgacacc
     2221 caataacacc aaaagtcccg cgccaatttc gcccgtcact tctccctcca cggtgacgct
     2281 ggcacgggtt acgcgttgaa ttaatgcaat catggttcgt cgtcttcttc ttgttcagct
     2341 gcttgtttta gtttgcggta ttccccgaga gtgacagtaa tttccgcgcc aagcaagacg
     2401 atacaccatg tccagtagac ccaaacaaag agaatgggga tcaccgccag cacaccgtaa
     2461 atgagctgat atgacgggaa catggtgata taaagcgcga aacctttctt tcctgcttcg
     2521 aacaggagtg cggcgacaaa cgcgccgaca atcgcgtcgc ggttaggtac gcggatggta
     2581 ggaacaatgc tgtacagcaa ccagaaggag atccacgaca acagcaacgg aaaaatacgc
     2641 agcacgttgt cgatgacagt attgagatcg ctcgcccagc gcagggagag caaataggaa
     2701 ctgatcgcca gactggcccc tgccagcagc ggccccagcg ttaaaatcat ccagtacacg
     2761 gcgaacgagt aaatttttgg tcgcgctcgt ttactgcgcc agatggtatt caacgcgcta
     2821 tcgatggagt acatcaacaa taacgcggtg acgatcagcc cgcacgcccc aacggcggtc
     2881 atcttgttgg aattggcaac aaattgttcg atataccgct gaataacatc gccagtagca
     2941 ggcagaaagt tggcaaaaat aaagtgacgt aactgaatgc tgacgtcgga aaacatggga
     3001 aaagcggcga aaagcgcaaa aacaacggca accagcggca ctaatgagag caacgaccac
     3061 ataggcaagg ttacctgcca gggttgtcat gttgtcctca tcaatacgtt gccagagtag
     3121 ttttagccag gcccatagtg gacgggtacg gtgcctggct ttgtcctgaa tggtttttag
     3181 cataacacct tcgcgaaata gtccgggatg gtggttttat ctttcaccag aatactggta
     3241 atgcccagct gattggctcc ttctatatta tcggcgttat cgtcgaaaaa gaccgtatcg
     3301 ctgggtgaaa aaccttccgc ctgcaaaaca tgctggtaaa ttcgtgcttc aggtttgcgc
     3361 atccccagat cttgcgacag atagatatgg tcagcagcat cacgaatttc cgggtattct
     3421 tccggccaga aggtggtatg caggcggttg gtattggaaa gcaccaccac gcgatgcccc
     3481 tgctcacgca gtttatgcat gatggcgatc acttccgggc gcagcgcaac aaacaccgcc
     3541 tgccagccgt gagagaactg ctcgtagctt agcggtagag ccatctcatg acacagcgcc
     3601 tctgcgaacg cttcgtcgct aatttcccca cgctcatgct gatgaaacgc ctcccccata
     3661 tgaaaactct tcttaagcgt agccagtgga acacgcgtta aatcgctcca ggctcccagc
     3721 acacggttaa agtcgatatc gacaatcaca ttacctaaat caaagatata gagcattttt
     3781 gcctcctttc gcgccacaag aaattaactg tagcgggaaa ggatgggttt ggctatgtgg
     3841 caataagggt aatcttgtga tgctgattgc gaaaaagcag agtaatttca ctggcttttt
     3901 cgcaatagat gaatggttaa gattcaggtg taaattgcca acgagtttcg atgaccagcg
     3961 gcctatctgg tgtcaccaat tccggcgtta gatttattgc gttaggtgcc tgagtcagcg
     4021 ggttaataca ggtggcatcg ggctgtttat caaataccac cagactgttt gcagaagaga
     4081 tcatggtcat cgcgagttta ccaggccaca gtaacttaac cttcaccccg ttataaaaac
     4141 caaaacagtc atcccaggga ccggcttgag gagagatacg atttccagtt ggcagttcat
     4201 cactacccgc ttcttcttgc cagtccgcat caaaaagcac ctgcaacgat tctgtatttt
     4261 gcggagtcag ctttttcgca aaccacggat gccagccagc ggaggccggg aatgtatcgg
     4321 catatgaatg gacttccagt tgcaaaacca gcgcatcgtt ctccagcaaa aaagtctgaa
     4381 tcacttcccc ttgccagggc cacggtgaag ccaggggcat acgtaaagtc agggaatctg
     4441 ctgttttgtc gatgatttcc cacgttgagt aacaagccat accatgcaac gcatgtggtg
     4501 gtttattggc gggtaaagaa taacattgcc caccagcatt cagcgtcgca ttccccagtc
     4561 gccctgccca cggcaccatt gggaaacagc catactgaaa agctctgcgc tgcggctgcc
     4621 attggcgtaa tacttcagaa ccaaatgcct ttaacgaggt tatcctggca ccatcatgcg
     4681 gataaatctg caggtgaata ttgccttttt ccagcgtcac taactctcct gcaggcccag
     4741 gccatggcgc gtcaaacgcc tgttgtagcg ccaggtaatg ctcgtttttt cgttcagttt
     4801 tcatgagatt gttccctgat gtagcggtaa gaagcggttt cttgttcatc ggtcggctca
     4861 atcagccaga cgcaaacaaa ggaaagaact gagacagcaa ataaaaacag agcgacatac
     4921 cacggttttc ctccccctag cgccagcaaa cttgaggcga taagggggct tagtccgcca
     4981 agaatcgcgg aaaattgata agcaaaagaa aggccggtat atcgtacttt ggtaccgaac
     5041 atgcgggtga aaagtgttgg ctgcacggca aacatcatcg tcggacctaa gttgtagccg
     5101 aggatcatac tgcaccagat gatgagcgta cttttgctat ccagtaataa gaagaaagga
     5161 aacgcgaaca ggacacaaaa tcctgctccc gacaaataca gacttttttg acctatacga
     5221 tctgataacc atccaaccaa tggacaactg aaaataccga tcgccgaggc aatcaacata
     5281 cccgtcaggg gaatatctcg cgacaatact aattggctgg aaatatag
//
LOCUS       DAJTOR010000224         5278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTOR010000224 DAJTOR010000000
VERSION     DAJTOR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.226"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1124
                     /locus_tag="OO249_003843"
     CDS             <1..1124
                     /locus_tag="OO249_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5637964.1"
                     /translation="EPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKT
                     ATGVIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNV
                     TFPSTMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKA
                     GAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQE
                     QAPTLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHD
                     SKFDLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIK
                     AIFGDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1160..1363)
                     /gene="ydfZ"
                     /locus_tag="OO249_003844"
     CDS             complement(1160..1363)
                     /gene="ydfZ"
                     /locus_tag="OO249_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5637965.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1541..2225)
                     /gene="ydfH"
                     /locus_tag="OO249_003845"
                     /pseudo
     CDS             complement(1541..2225)
                     /gene="ydfH"
                     /locus_tag="OO249_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
     gene            complement(2314..3060)
                     /gene="ydfG"
                     /locus_tag="OO249_003846"
     CDS             complement(2314..3060)
                     /gene="ydfG"
                     /locus_tag="OO249_003846"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5637966.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3197..5242
                     /gene="dcp"
                     /locus_tag="OO249_003847"
     CDS             3197..5242
                     /gene="dcp"
                     /locus_tag="OO249_003847"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5637967.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1314 a         1262 c         1374 g         1328 t
ORIGIN      
        1 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
       61 cggcgaccgg acagttaatg ctcgatcacc ctatggtcgc tgccgacgtg caaaatcccc
      121 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggcccgccgt aaagcggcag
      181 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
      241 gtgacgttgt cacttcatac gcgaaagctg ttgatgtaaa actggcacaa tggatcgaag
      301 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
      361 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgcgggcgtt gcctgtgaac
      421 ctttccgcca gtgggtaata gaagataact ttgttgccgg acgtccagaa tgggaaaaag
      481 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
      541 acggcagtca ttcattcctg gcgtatttgg gttatcttgc cggatatcag cacattaatg
      601 actgcatgga agatgaacat tatcgttatg cggcgtatgg cttgatgttg caggaacaag
      661 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgaac
      721 gctatagcaa tccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggtagccaga
      781 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
      841 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
      901 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
      961 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     1021 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     1081 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     1141 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     1201 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     1261 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     1321 tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc
     1381 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     1441 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     1501 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     1561 cgctgttttc ctggcggatc tggccacgga ttcgctgatt tcctgcaaat gttgcgtcat
     1621 tgcacgttct accgcatcgc catcacgttt ttgcagggca gagaaaatat caagatgctg
     1681 gcgtaacagc atttctggtg gagaaacatg gtcgaaactc atatagcgca cgcgatcaac
     1741 ggtcgctttc aggttctcaa tggtatccca cgccagttga cagtcggcaa tctgcgtcag
     1801 gagttgatgg aagttgtcat caagttcaaa aaatcatcca gttgcttgtg ctcaatggca
     1861 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     1921 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     1981 tgggccatgg aaattttgtt gacgtagctg ccacgctgtg gacgaatttg aatcaggccg
     2041 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     2101 gaaacttctt tttccgacaa cggtgtgcct ggagcaatca ggcaatggac aatgtcgcga
     2161 cgaaggatac gataaatttg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     2221 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     2281 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     2341 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     2401 tgacacccac caaacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     2461 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     2521 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcaggt ccgtacgcag
     2581 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     2641 aggccagcta cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     2701 acccggtaag acggcgcgcg tcatatatac caggcctttg ttgttggtat caatcatcgt
     2761 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     2821 atttaccagg atatcaatat tgcaccactc ggcaggaagc gatgccagca tctcttcaat
     2881 agcggcgcgg ttgcgaacgt ccagttgggc gatgtacaga ttatctccca gttcgtcttt
     2941 taactcctgc aaccgttcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     3001 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     3061 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     3121 taatactggt ttgtaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     3181 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactaccgta
     3241 tctggctccc cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     3301 aatgcagcaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     3361 caacaatact attctggcac tggaacaaag cggagaatta cttacccgcg ttaccagcgt
     3421 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     3481 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     3541 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     3601 ggtggaggtg attcatcaac gttttgtcct tgccggagcc aaacttgcgc aagctgataa
     3661 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     3721 attactggca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     3781 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     3841 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg agatgcgcga
     3901 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     3961 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     4021 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     4081 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg ctagcgatga
     4141 attagcctcc atacaggcgg ttatcgataa gcagcaaggc gggtttagcg cgcagccgtg
     4201 ggactgggca ttttatgccg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     4261 gctcaagcca tattttgaat taaacacggt gttgaatgaa ggtgtattct ggaccgcgaa
     4321 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt cctgtctacc atcctgacgt
     4381 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     4441 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     4501 gcttaatgaa acgcatccgg taatttataa cgtttgcaat tatcagaaac ccgctgccgg
     4561 tgagcctgcg ttgttactct gggatgatgt cataacctta ttccatgaat ttggtcatac
     4621 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     4681 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     4741 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     4801 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     4861 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     4921 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     4981 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     5041 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     5101 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     5161 tctggaacgc ctgtatcgac aatggcgcgg taaggctcct cagattatgc cgatgctgca
     5221 acatcgtggc ttgaatatat aaacaccacc aatggctggc ctggagatat cgccaggc
//
LOCUS       DAJTOR010000225         5229 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTOR010000225 DAJTOR010000000
VERSION     DAJTOR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.227"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..356)
                     /locus_tag="OO249_003848"
     CDS             complement(<1..356)
                     /locus_tag="OO249_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5637968.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGF"
     gene            complement(361..1011)
                     /gene="hyfE"
                     /locus_tag="OO249_003849"
     CDS             complement(361..1011)
                     /gene="hyfE"
                     /locus_tag="OO249_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5637969.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(1023..2130)
                     /locus_tag="OO249_003850"
                     /pseudo
     CDS             complement(1023..2130)
                     /locus_tag="OO249_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(2142..2252)
                     /locus_tag="OO249_003851"
     CDS             complement(2142..2252)
                     /locus_tag="OO249_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR5637970.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(2269..3216)
                     /gene="hyfC"
                     /locus_tag="OO249_003852"
     CDS             complement(2269..3216)
                     /gene="hyfC"
                     /locus_tag="OO249_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5637971.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(3227..5229)
                     /gene="hyfB"
                     /locus_tag="OO249_003853"
                     /pseudo
     CDS             complement(3227..5229)
                     /gene="hyfB"
                     /locus_tag="OO249_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
BASE COUNT         1460 a         1476 c         1368 g          925 t
ORIGIN      
        1 aagaaaccgt agtaatcgca cagtgtaacg ggtgaaagct cgccgtgtgc cacttcatga
       61 cgcatgtagc caatcgagta aacaccggtg agaaagccaa tcacaccaag aatggcgagg
      121 aacaaaccgc ccagaccatc aatatgaagc cacagtcccg cagcgaatat ttctcctgca
      181 tcagcggcag tttggaccac ccagagtgcc agaatcagca gcagtgtgat ccctaagcta
      241 tgtaatacag tcaccgtaca agtcgcagag agcccccgtt tccggcaggc aaaacagagc
      301 agcgaaaaaa gcagcggcgt gagcaggagt aaagcgaaca tcacagaata actcatctca
      361 ttatcccttc agcgcggtca agttgttcac gtccagcgtg ccgtgggtac gccatatttt
      421 tcttgccagt aacaccatca caatgacggc gaagatggcg tcggtagcga tacctatttc
      481 caccagttcc ggtgctcgcc aggcaagaag cgccagcacc agatgggagc cgttttccat
      541 caggcagtaa ccaaaaattt gccgcaggat attgcgctgg ctgacaatgc acagcaggcc
      601 aagcagaaaa tgacctaacg ctaccgccag cgccggtttc agcccggtag ccatcggtag
      661 cttcacgggc tgaacgacaa atgcgcaaag caggacaatt aacgccgcga gcagtgccat
      721 cattgccgga ccgaataacg ctttttccgg gatgttctgg ggaatatttc gtgcagcgta
      781 agtcatgatt aacggtacca gcagcacttt ggtgataaag gcgctggcgg accagatcag
      841 cagttgctct gcggcgaaca ggcacgagag agtggcgaaa atagacacca gcaccagcga
      901 ctggcaggcg taaaatccgc atgacaggcg atagcttttg acgctaatca caaacagcga
      961 tgtcagcatc atcagtcccg ccagattatt tacgatcata gaaccgatca ttcatcatct
     1021 ccttactgca accaggtcgc ggcgattacg ctggaaatca gcgacatcac aatcagtaca
     1081 atcaacacca ggcgcattga ccctggcagc ggtgcggcat cggcgacggc ctcgctcggt
     1141 ttgccaggca caacgcgacc aaaccagcga ataaaccagg cgaaactggc gaccgattca
     1201 atcatcagca gaatcatggc gggcagcagg atccagtact ccactgacaa cgcaaaaccg
     1261 gcggcaaaca gcgggaattt actgaagaag ccgttgaacg gcggcacacc ggtaatcgcc
     1321 agcgctgcca cgcagaaacc cacgcctggc aacggcaggg agtgcaatac gccacgcaga
     1381 cgcggcaaca agcgcgtgcc gcagctgtaa ctcagcgcac ctgctacaag gaaaaacagg
     1441 cttttagcga acgcgtggtt gacgatgtag gcgatgctgc cctccagtgc cagccgccca
     1501 ctttcaccat cgatgcggcg tggagatagg ctctgatcgg tgttggcgct tccattgcgt
     1561 ccggtagcca cgcttgcatc ggtagctggg ccgatttccc ccacgcggca aacaggatgc
     1621 cgccataaac cagataacgt gcgtcgccat gtaactcgct catcgcgcta agcacaaacg
     1681 ttccggtctg caaaaacagc gtggcggcgg caagatacaa ccccaacgaa ccgatatggg
     1741 tgataagcaa cgcttttagt gttgaacgct gcgctttatc gctctggtaa taactgatca
     1801 acgcccagga gcagccgccc taatttcaaa aaacaacaac tgaccgagca gcgtcgagga
     1861 gagtaccagt cccgccatcg cgccgataaa caccagcaaa aatgcgtaat aacgattcgt
     1921 gccgttatgc gggtgttcgc gatttttatc cgtcagataa cccgtggagt agatcgtgac
     1981 cagcaaaccg agaaacacca ccacaaacag aatcagcgta ctcacgcgat caatgaccag
     2041 gccaaacaat gccacatccc ccacgttgac caacgtcaac gtgacggcaa ctttatcggc
     2101 ctgataaaac gcggagatca gcgacaacat gcacagcgtg gtcagtgcgg cgaacaaaac
     2161 ccccattcgg cggcccgacg ttgtggcgaa aacgaaacga ccagtgcgcc gataaaaggc
     2221 agcattaacg tcgtcagagc aagattttcc atattccgtc agtgctcctt acagaccggt
     2281 taaccagaag acccatgcaa gcgcagcaag gctgaagcca agccaggtca catggtgaat
     2341 gagtaaaaaa cgcccgcgtg ccagcgtgtt ttctgcgatt gaggccagta caaaaatcag
     2401 caaaaccttg agcagcgtaa cgacaagtga agtcagcagg caggcgagag aaagttcttg
     2461 cgcgcgccca aagggcagaa acagggccac aaacagtgct gccatcacga cctgtttcag
     2521 ccccagcccc catttcgcta gcgccagccc ggcaccggaa tattcggtca gcgggccttc
     2581 ctgtaattcc tgttctgctt cagcaacatc aaagggaatt tttcccatct caatgaagca
     2641 ggcaaaacca caggccagta acgccagtac ggtggttagt ggcgagttcc agcccgttgc
     2701 cagcgtattg ctgatcatct cgatatgcgt ggaacctgct atcagcgcca ataccagcag
     2761 tgagagaatg agcatgggtt cgaccagaat gccgagcgtc aactcgcgac tggcaccgac
     2821 tcccgcaaac gggcttccgg tatccagtcc ggaaagagca aagaaaaaac gaaacagggc
     2881 aagaagatag ataagggtga tcagatcgcc gccgcccgca aaaggggaaa cggtaataaa
     2941 aagtggtagg gccatcgcca gcaccagcat gctgctgatt aatacccacg gcatcaggcg
     3001 gaacatcaga cctgaagata taggcgcaac ttcctggcgt ttaaacagtt tgtggatctc
     3061 gcgataatcc tgccagatcc ccggcccgcg gcgggagtgc atacgcgcgc gtatctgccg
     3121 ggaaataccc gtaaaaagcg gggttagcag cagcagaatc acggcctgtg ctaacgcaaa
     3181 aatgaccaga tatccgtcgc aaagagtttg tctcatggtg atttccttag acggcaatag
     3241 cgattagcag cacaaccagt gcggcaacta gatacaggca atagagacga aagtcgccgc
     3301 tttgcagatg ctgtatttct ttggccagcc gttgggtggc gctcacgatg gggcggatca
     3361 cccgctcatc ccagaagggt tctgtgctct gagccctggc ggtgacgtgc gcaagatctt
     3421 tatttagcct cagcgtaggg tcgagctgtt gtcgtagacg aaataacgca gagaagacca
     3481 cacgcagcgg ctgcatcacg ccattgcctg acggagccat cgcgttttcc cagccgtagc
     3541 cgcatgccca ggcatctcct gtgcgacgaa acgctgcgcg gcgcgaacga caccacagcc
     3601 agtaaaggcc aggcagcaaa ggcatcgcca gtagtaacag caacagtaat gaaggagtga
     3661 cccgtgtatg aaacgtgccg ggtacaagtg ctattccgct ggcgacagtg acgggagggg
     3721 tattggtaaa cgcatgggca atatgcatta tcttcggtgc cagccaactg gcactaagcg
     3781 caattaatac gcagagtgcc gcgagcagta gcatggcgac gatcatcgta tttggcactt
     3841 cctgtgcctc ttcagcgtgt gtactgcgcg gcgcaccaca gaaagtaata ccgtacattt
     3901 tgacgaagca cattactgcc agcccaccag tgactgccag cattaccata gcaataggac
     3961 ccgcaagttg tagcgctacg gcttccacac gacttagtga gaacagcgac tgccaggtgt
     4021 accattcgct gataaaacca ttcagcggag gaatggctga tatcgcgagg caaccaatca
     4081 ggcatgctgc ggctgtccac ggcatccgtt tcgccagtgc ccccattttt tccatgtcgt
     4141 gggtatgcaa acgcgaaata atcgcgcccg cgccgagaaa tagcagccct ttgaacagcg
     4201 catggttgag cagatgaaac agtgcgccga gcagtccaac cacggtgagc agcgggtcgt
     4261 gcagtgacag accgtccatc gccacaccga ctgccagcaa aataatgccg acgttttcga
     4321 cggtactcca ggccagcagc cgtttgatat cctgttccgc cagcgcatat agcacgccca
     4381 ggagcgcgga gattgcgccg atcgccatca ccagaatgcc ccaccacaga ggcaaacccg
     4441 tttgcgccag cagatccatc gctactttca ggatgccgaa aataccgatt ttgaccatta
     4501 cgccagacat caacgccgaa gcgtgcgatg gtgcggcagg gtgagcgcgc ggcaaccagc
     4561 tgtgcaacgg catcatcccg gctttcgcgc caaaaccgaa aaaggccagc agaaacatcg
     4621 ccgacgccag ccccggagaa agtgaaagcg tgcgaaactg gcaaaatcga ggctgccgct
     4681 ttcgcgcccc atcagcaaga aggcgatcat aatcagcacc gatccggcgt gggcgataaa
     4741 aaagtagagc atgccagcgt tgatcgacgt tttatcctgc ctggcaatga ccagaaacca
     4801 ggaagacagc gacatcattt caaacagcac gatgaaccaa aaagcgttgt ccatcaccag
     4861 cagggcaacc atcgatgcga tgaaaagatt cataaagaag cccatcgccg ccgcgccttt
     4921 gccctcgtat tcgcgcatat aagtcaatga atagagcgaa caaaccacca ccagcaagga
     4981 gataaccagc accataaata ccgagaggct atccattcgc agggttaaat cggcaaagtc
     5041 aaaaggggtg gcaaaacggg cgactaaagt tacaccagca tgtaattgcg taattccgct
     5101 gatgatgcca atcacgccgc ccaccagcga tgtgatgccg gaaagcttaa tagccagtct
     5161 gtccagaccg agtaaaaaca gcgaagccag actggcaaac agaaagagga tcagcgacca
     5221 ggttaataa
//
LOCUS       DAJTOR010000226         5197 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTOR010000226 DAJTOR010000000
VERSION     DAJTOR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.228"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..107
                     /locus_tag="OO249_003854"
     CDS             <1..107
                     /locus_tag="OO249_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR5637972.1"
                     /translation="EQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(157..1905)
                     /locus_tag="OO249_003855"
     CDS             complement(157..1905)
                     /locus_tag="OO249_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCR5637973.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLVLLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPDIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFLITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(1905..2975)
                     /locus_tag="OO249_003856"
     CDS             complement(1905..2975)
                     /locus_tag="OO249_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5637974.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQRPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQMD"
     gene            complement(2965..4416)
                     /locus_tag="OO249_003857"
     CDS             complement(2965..4416)
                     /locus_tag="OO249_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5637975.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4427..4873)
                     /locus_tag="OO249_003858"
     CDS             complement(4427..4873)
                     /locus_tag="OO249_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5637976.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1315 a         1341 c         1312 g         1229 t
ORIGIN      
        1 aagagcaact tcaggacgca aactcaagct ggatggtcga agactctttc ccgagagcat
       61 tacgcgagta caacgaaatg gttgatgact ataatagcct gcgttaaacg tttttgatga
      121 taaatgcagt gtcggatgcg gcgcatctga cacagttcac cccttgtgaa gtaactccat
      181 aaatgcctca tatcctgtta acccggcaat aacctctgcc ggatgctgac acaatatgct
      241 tgccagatag ctaaatattt ttagctcatg acgcgcatcg gcggcggcgc aggcgatcaa
      301 gacatggtta atgacttcgt tattcacctc aactggttgg gcgagggtaa taagaaatcc
      361 acgaaaacgt cgctcctgtt cgctccagca atgtgggatg gcgaggcgat taacaatcag
      421 gttttcacct tcgccttcgc gggcgatgat gcgttgtgct tcatcggcgg taatatggtg
      481 ttgtgcgact aattgcgcac aaatttgccg ggtaatatgt tgccagcttt caccgcgtac
      541 attcgaataa tgaaaacttc cgggggcaga gaaaaaacgc tccggttgct ggcgaataaa
      601 tgccgtcgcc agaaaatgtt ttatttgttc gataccggca gccgtaatga tattttttac
      661 ggtgatgtaa ttatttaccg cgtcttccag taaataatgg ctattgttaa tgatcaataa
      721 cggctcaatc tcttcgcgaa tggcaacaag ttcgcttaag ctacgggcaa taattacccg
      781 acaatttaaa acatcgcgct caatggcgag ctgattaata gtggcaatcg cgttctggtc
      841 cgagagcaaa atgatcggct ggcgttcgtt ttgatgccgc tccagcgcac aggcaaaata
      901 caaaccgatc agatcgctgt cgaaaagggg aatatcgagc tgttcacgca gtagcgtaat
      961 aaagtgcaga ctcatatcaa aggccgcagg ccaggctgct ttcaggttat tcatgctact
     1021 ttggcggtgc tcagcaatcc acaccggtgc ggcaaggcaa cgtaataaat gcccggtaat
     1081 attctcaacc aactgcgtat caataatatt caatgacgcg atgtctggca ccctttgcag
     1141 caaccccagc acatgatcgg ctgaaattac ctgcgcttgc tgatgctgtt tctctatcaa
     1201 acctgtcagg gttcttacag cgttatcacc aagaaacatg ccgctttgtt caacgatttg
     1261 ttttatctcg tcaccgggat attgcggcca ctcatcggtc agttgattac gcagggcgta
     1321 aatcgccaga atcaggctgg agagataatc accctgcatg agcggccagc ggtgttggtt
     1381 gtcgcaggcc gtggataaat gctgaaggtt gtctcgtgtt acgcccgcca gcggaattaa
     1441 aaacggatct ttgcgcagca agttcgccag caagataacg cgcttctctt ctgtctcatc
     1501 aatgaaatgg cccaaaccgg ggcggctggc caggcaacaa gcgcgttcat aacgctgttt
     1561 taaccggggc agacgctctg cgacccacgt ttctggcaaa ttaagcgccg aggcgagttg
     1621 cgcacgggga gtaaaggtat tcagtaataa cagtaccagc agccgatagt cgttatcgtg
     1681 cttttgcagc aactgaaaaa agctgcggcg ctcgaagatt tccagctgat agcccgcact
     1741 accactggcg gaaatgcggg ctttgccgtt aagggtaaag ttgagatagt caatatcacg
     1801 caggatggtc ctgcctgaaa cgccagtctg ttgcgccagc tcaccaggcg tgcgcggctg
     1861 ttgctccagc agatcgacaa tctttaactg gcgttcgttt agcattaatc catctgacgg
     1921 aactggctaa acgcgttgac tttctccgcg gttaaggtgg tcagaaaatc ccgtatcaac
     1981 gtgagcagag catcgtaatc ggcttttgaa atcataccgc tgttagcgtg cagatagcgg
     2041 gttggcaggc acagcgcaac aaccggacgc ccgccgccca tcacgttgta gcgcccgcca
     2101 tccgtcgcac cggttttcat ggtggaaaat tgcagcggta aatcgttatg tgcggcacag
     2161 ctttttaacg ctgctaccag tttctggttg gggaagtagc gcttgtcaaa cagcatcagc
     2221 cccggcccct ggcccagttt cagcgggtat ttaatgttat caatgcccgg aacatcgcct
     2281 gcgacagcgg tatccagcac gatcactaca tccggtttaa tgtgttccgc cgaggtttgt
     2341 gccccgcgta gcccaacttc ttcttccacg ctgccgacgc catacagcgt aatttcggga
     2401 ttattcaccg tctgcaatag ttcagccatc attgcgcagc cgatgcggtt atccagcgcc
     2461 ttgccgacca ctttatcttc gccccagcag gcaaaattgg cttccgggct aataaaatcg
     2521 cccatcgcca caccgcgttt ttccacctca tcgcgactgt ttgcgccaat atcaatgaac
     2581 atctcatcaa atgacagcgg ccgttgcctt tgcttttccg ttaacgcatg aggcgcgacg
     2641 gaaccaatca caccagggat tttcactccc ttgtgtgtgc gtatggttac ccggtggttg
     2701 agcatcgact gattccacca gccaccaatg gtggtgaaac gcagaaaacc gctctcgtcg
     2761 atgtgggtga ccataaagcc gacttcatcc atatgcccga caacggcaac ttttggacct
     2821 ttattcccct tacgggcaac aaagctgccc agaccatcaa aggtgatctc attaacgcaa
     2881 ggttccagcg tgtttatcag aatgtcgcga acttcctgtt catcgccgct gacggcgctg
     2941 gcttcgcaca actgttgcag taactcaatg ttcatggaca agctcctgtt gcgcggcttt
     3001 cgctttgcgg cgtaaccaca atcctttcag gacgataatg aatgaaatgt tcagcgcgag
     3061 gccaatcgcc agcaccagat aaaacgaacc caccggcgac atcaggccga tcagcgggtc
     3121 aaagataccc aaaccaggtg ccagacgttt gattccgaaa gcgatcacca gcacgccggt
     3181 aatgccaccc gccagcgtat tggcggtaat catcggcaac ggagctgcca gagcgtaagg
     3241 tatagccggt tcggttgcga ctgtggcacc gaccacaatg gcactactgg cagcctcttt
     3301 ttcctgtcgg gtaaacagtt tgggcgcaat gaacgtcgca agacccgccg caacgggcgg
     3361 catcaacgcg acaacgccga cgatggcgta ccagtcataa atgtgttttt ccagcagtga
     3421 gaagcagaag aaccaggcgg ttttattgat ggggccgccc atatcgaacg ccagcatcgc
     3481 accgaccaga aacgctgctg caaacttcat tgatggagga atggtattca ggaagtggag
     3541 cagcccgccc atgaggtcgg acatcaccgg cccgataacg tagtaagtga gcacgccgaa
     3601 aaccagcaga gtaacgaacg ggatcagcat tgaaccgagc agcggttgta atgctttgcc
     3661 aaggcgcact ttgcggaacc agaagacgaa atagccaatc gccagcccca gcaccaccgc
     3721 gccgaggaat cccgcacccg actgggtgcc gagcagcgct ttgtcgttcg ccagatagca
     3781 aaccagaaac gccggagcga aagccggttt atcggcaatc gacgaggcaa tataagcgcc
     3841 catgatgggg atcataaagg tgaagccaag gtaaccgatg gattcaacta cccaggtaaa
     3901 cgacggcgcg cctttcgaca tatcggtata cggcaaaccg aactgcacca gcatgttggc
     3961 aagcgccacc aggataccac cgccgatgac aaacggcagc gccgcagaga cgcccgccat
     4021 caggtggctc atcacgctgc cgctttgcat ttcctgtttg ccaagcttca cgccactatc
     4081 tgcggcaaaa agctgcgagt ttgtcggtaa ttcgctgaaa atctggtcga tatttttcaa
     4141 cgcctgggag atggcaatct cataaacttt cttcccggta aaccgcgcgc gatcatcacc
     4201 gctcaggcca cgcccggtag cgagaatgac gtaatcggca gcggcaatct cttcgctgga
     4261 taagcggttt tcaacgccac tggacccttg agtttccact tttatggtat ggccgagaga
     4321 acgggctttc tgttccaggg cttccgccac catgtaggtg tgagcgatcc cggcggggca
     4381 gttggtgatc gcgacaatac gtaaggatga ctccatcgcc acttccttat gagctgaggg
     4441 tttgatttaa caaggcaagc acttggtcgg tatcgccctg ttgcagttga tgaataaatt
     4501 ccttgtgaat aattttgcga cacagtgtgc cgatgatttt gacctgatcc tcttcgccgc
     4561 tttgcggcac gccgaggcag atccagcaat tgacgtcttc gccatcgctg gctttccagt
     4621 caatagcctg cgctttgcgg gcgaataata cgaacggttg tttaacgcag gcgcttttac
     4681 cgtgcggcac ggcgacaccg gaaccaaatc cagtagagtg cattttttcg cgcagcagca
     4741 gggtttgcag gaactggtgt gagtcggtga taaaaccgtt ttgtaacgct attgtcgcca
     4801 actgcttcag aacggaataa gcgccattgc cctgaatatt caggtcaata cagcttgcag
     4861 taagagctgc cattaatcgg actccataaa atatatttta atcaattgta atggtggagc
     4921 gattaagtga tgagaggcga tatgtcggta aacaatgaca atttgagaca gagtgaaagg
     4981 tcagatttgc ggagtaatgc acataatggt tatttaaata aaccacatga atcattaatg
     5041 ggttattcat tatttttgtg atttactcac aatgtatcag ggaaatataa cttaccggga
     5101 gatgtaatat gtttaatttt tcaaatcgaa atttaaaata ttgtgccgga ggcatctccg
     5161 gcacattggg caattacggc aggtaaaaca cttcttg
//
LOCUS       DAJTOR010000227         5186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTOR010000227 DAJTOR010000000
VERSION     DAJTOR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.229"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1076
                     /locus_tag="OO249_003859"
     CDS             <1..1076
                     /locus_tag="OO249_003859"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5637977.1"
                     /translation="EPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTCYKE
                     KVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALA
                     VQTGKSINPEFNIGCMIAMYPIYPLTCVPNDMMMATKAMHRRYWFTDVHARGYYPQHM
                     LNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVS
                     NPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRI
                     DYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGT
                     LERIRKASFYWYRDLIANNGENI"
     gene            complement(1133..1351)
                     /gene="marB"
                     /locus_tag="OO249_003860"
     CDS             complement(1133..1351)
                     /gene="marB"
                     /locus_tag="OO249_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5637978.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1383..1766)
                     /gene="marA"
                     /locus_tag="OO249_003861"
     CDS             complement(1383..1766)
                     /gene="marA"
                     /locus_tag="OO249_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5637979.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(1786..2220)
                     /gene="marR"
                     /locus_tag="OO249_003862"
     CDS             complement(1786..2220)
                     /gene="marR"
                     /locus_tag="OO249_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5637980.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2432..3097
                     /gene="marC"
                     /locus_tag="OO249_003863"
     CDS             2432..3097
                     /gene="marC"
                     /locus_tag="OO249_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5637981.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(3122..4312)
                     /gene="ydeA"
                     /locus_tag="OO249_003864"
     CDS             complement(3122..4312)
                     /gene="ydeA"
                     /locus_tag="OO249_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5637982.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4462..>5186)
                     /gene="yneK"
                     /locus_tag="OO249_003865"
     CDS             complement(4462..>5186)
                     /gene="yneK"
                     /locus_tag="OO249_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5637983.1"
                     /translation="PKPVTMGRVFHTGVERVLFLFLNDFIEQFPMIHLGVPIKKAHTP
                     HIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWSNSEVHRWFVNKDPNLITA
                     ILDITDGELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDFFLFFANML
                     LHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHEDLVKNR
                     PRSPVRPPIPATAKTPDLPERH"
BASE COUNT         1392 a         1170 c         1273 g         1351 t
ORIGIN      
        1 tggaacctgt ggtgacgctt tcacattttg agatgcctta tcatctggtg acaaaatatg
       61 gtggctggcg aaaccgtaaa ctgatcgact tttttatccg cttcgcatca acggtcttca
      121 cgtgctataa agaaaaagta aagtactgga tgacgtttaa cgaaatcaat aatcaggtga
      181 atttcagcga aagcctgtgt ccatttacta attccggtat cttgtattcg ccagaggaag
      241 atatcaatga gcgcgaacaa ataatgtacc aggcggtaca ttacgagtta gttgccagtg
      301 ccctggcggt acagactgga aaatcgatca atcctgaatt taacattggc tgtatgatcg
      361 ccatgtatcc catctatcct ctgacgtgtg tacccaacga tatgatgatg gccacgaaag
      421 caatgcatcg tcgttactgg tttactgatg ttcatgctcg tggatattat ccgcaacata
      481 tgctgaatta ctttgccagg aaaggattca acctcgatat cacaccagaa gataacgcga
      541 ttcttgccag tggttgtgtc gactttatcg gctttagcta ctacatgtct tttacgacac
      601 aattttcgcc cgataacccg caactggatt atgttgaacc acgagatttg gtcagcaacc
      661 cttatatcga tacatccgaa tggggatggc aaattgatcc ggcagggcta cgttattcac
      721 tcaactggtt ctgggatcat ttccagttgc cgctgtttat tgtcgaaaat ggatttggtg
      781 cggttgacca gagacaagct gacggcacgg tgaacgatca ctatcgcatt gattactttg
      841 cttcccatat tcgggaaatg aaaaaagccg tagttgaaga tggtgttgac ttaattggct
      901 acaccccgtg gggctgcatt gacctggttt ctgccggaac aggggaaatg aaaaagcgct
      961 acggaatgat ttatgtcgat aaagacaacg aagggaaggg aacgctggaa cggatacgta
     1021 aagcgtcgtt ttactggtat cgggatctca tcgccaacaa tggcgaaaat atttgagact
     1081 tgaggaagat attacggaga tgtcaggctc tgtgatcaga gcctgacaat aactacatag
     1141 cgtgttgatt ataatagggc acgccgagcg catccgactt atcactgcca gtacccatgt
     1201 gatttaaatc aaacggtggt tgttcctgcg atgggggaac aaccacgaca ttggcacaag
     1261 aagtaacaac tggctgcgtg gtttgttccg caacgccctg cgcggaaaag agaataagcg
     1321 cagctgctat tgcggatgaa agtggtttca tgattgcctc agtgacgttg tcacgttatc
     1381 aactagctat tgtaatgatt taatggatgt aaaaagcgcg attcgccctg catattggtc
     1441 atccggtatt tatgtggcgg aacatcaaag taatttttga aggttcgggt cagagtttgt
     1501 tgcgactcga agccatatcg ttctgccaga tagagtatcg gctcgttact ttccttcagc
     1561 ttttgcgcga tttccgtcat cttacggctg cggatgtatt ggcctaatga atgaccggtt
     1621 tcttttttaa acatccgttg caggtgccat ttagagtaac ctgaacgctc tgacactttc
     1681 tccagtgaca gtggcgattc caggttgtcc tcgatccagt ccaaaatgct atgaatggta
     1741 atagcgtcag tattgcgtct ggacatcgtc atacctcttt tttgtttacg gcaggacttt
     1801 cttaagcaaa tgctcaagtg ttgccacttc gtccgccgtc aggttttttg ttaattcttg
     1861 atgcaggtcc tggccaacta attgatggca ttgttcgcat attgccgcgc cgctggtggt
     1921 aagttttacc agtacgccgc gcttatcatt cgggttcggc aacctttcaa cccagccttt
     1981 acagaccagg cgatccagca tacgggtcag tgctccaagg tcgacagaca acactttttt
     2041 cagttcaacc ggagtaatac acgccgcgca gcggatagag cagagcacct taaactgtgc
     2101 cgcggtaata tccagcggag acagatactc gttaagcaga cgatctttct tctgattaac
     2161 catatggatt aagcgaccca atggaataat ttcattgaac agatcgctgg tacttttcac
     2221 attagttgcc ctggcaagta attagttgca gggggtaata ttgcccaggc aagtataagt
     2281 caaataaatg aattgaccga tgccacgttt tgctaaatcg gttcaagttt tggtcaatgc
     2341 gatgcaaggt tagctaaaga gtgacacttt atccttgttt accccttata cttttcgctg
     2401 ataatccaga tacactggat aacaaccacc aatgttagat ttgtttaaag caattggcct
     2461 ggggctggtg gtgttactgc cgttagctaa cccattaaca actgtcgcgt tatttcttgg
     2521 cctggcaggc aacatgaaca gtgccgaacg taatcgtcag tcgttgatgg cctcggtgta
     2581 cgtatttgcc atcatgatgg tggcgtatta cgccgggcaa ctggtgatgg atacatttgg
     2641 catttcgatt cccggtctgc gaattgcagg cggcttaatc gtcgcgttta tcggttttcg
     2701 gatgctcttt ccgcaacaga aagcgattga ttcaccggag gcgaaaagca agtcggaaga
     2761 gctggaagat gaacccagtg ccaatattgc ctttgtgccg ctagcaatgc caagtaccgc
     2821 tggtccggga accatagcga tgattattag ctcggcgtcc acagtgcgtc agagttcaac
     2881 cttcgctgat tgggtactga tggttgcgcc gccgctgatc tttttcctgg tggcggttat
     2941 tttgtgggga agcctacgca gctccggcgc aattatgcgg ctggtgggca agggtggcat
     3001 tgaagcgatc tcccgtctga tgggattcct gctggtatgt atgggtgtgc agtttattat
     3061 taacggcatc ctggaaatca ttaaaacgta ccattaaaaa aggcccgaat gggcctttca
     3121 actattgcgt ctgttcttcg agtgtcactg gccagcggcg aaatataatg attgaccata
     3181 ttaacgcggc aaaagcaggc accgcgccca cataaccaat catcgacatt gaccagtgca
     3241 aactcacctg attacctacc aacgcacccg ccccgattcc aatattaaat atgccggaga
     3301 atagcgccat cgcgacgtcg gtagcatctg gtgccagcgc cagcacttta acctgcatac
     3361 caagcccgat gatcatcatc gcgatccccc agaaaatact cagcaccccg aggtgtattt
     3421 cactgttcgc cgcaggtagc agcagtgcca gacacaccag caacagcgca atcgccgtac
     3481 tcaccaacgc agacgcatac tgattaccca gtttaccgaa aatcacgctg ccaataatgc
     3541 ccgcaccacc gagtaataac agtaatgccg tggcaaagtt ggcgctgaat cccgcaatgt
     3601 tttgcacaaa aggctcgata tagctgtatg ccgtgtaatg ggcggtgaca accaccacag
     3661 ttaacaaata aatgctcatc aatgccgggc ggcggaacaa tagcgggagg cttttcaatg
     3721 aaccggaatg ctcactgggc agtaagggaa gtaacttaat caggcacaaa agggtgataa
     3781 gcgccccaat accaatcgcg aagaaggtca ttcgccaacc gaaatactgc cccacaatgc
     3841 gcccgagagg taaacctaag accatcgcca gtgctgtacc ggtggcaatt aaactcaagg
     3901 cctgtgcccg cttcccggcc ggagccatac ggatcgccag agacgccgta atcgaccaga
     3961 aaatcgcatg tgcaaaagcc acaccaatgc gactgatcac cagaacggta aagctccacg
     4021 ataaaaacga cagtacgtgg ctggcaataa acaccacaaa caggcagatc agtaatttgc
     4081 gccgttcaac ctgactggtc attaacataa aaggcaatga cattagcgct actacccatg
     4141 cgtaaatggt caacatgatg ccgacctgag cggtttgcat gtgaaaactt tgcgcaatgt
     4201 cagagagcag gccaacaggg acaaattcgg tggtgttgaa gatgaaggcg gcgactgcca
     4261 gcgtaacgac ccgtagccac gccactttgc gggaaacagt gtttgttgtc ataacagatt
     4321 atcgggtgag ttgcgaaaaa gtgatgaaat gatcttaaaa cggttaatga tgtgaataca
     4381 atgcatttgt gatctggatc gcgttttccg tttctctgac gctgattctg ttccgcgtca
     4441 gagagtcggt aacatcacgg tttaatgacg ttcagggagg tctggcgttt tggcggttgc
     4501 gggtataggt ggtctgacag gggagcgagg cctgtttttc accaggtctt cgtgagtaac
     4561 ttttggcggc gtttcatttt ttacaaacag tttgagggtc ctgcgcgcca tatgcttgat
     4621 aattttatca tcacttaatg atgctggcat caacacgtca ttcagatcac tttcattggg
     4681 attcataaat tggtgatagt gcaataacat attggcgaaa aataaaaaaa agtcctgaaa
     4741 atgaggagat tcttgttctg ctaaaaggga taaaaagcag atgcagactt cgggtagtac
     4801 atgtttgtta gaacctaatg aatccatcag gctttgcaat aaatcctctt ttaattcgcc
     4861 gtctgttatg tcgagaatgg cggtgatcaa gttaggatct ttattgacga accatctgtg
     4921 aacctcagag ttactccata atcgggggag tatgacaaaa ttaccgcgag agataaaatt
     4981 caggacaagc aaatcaaact ggcgtaagta atctgcagcg gtatggtgat ccggaggcag
     5041 gggttcaata tgtggtgtat gcgctttttt gatggggacg ccaaggtgga tcattggaaa
     5101 ttgttcaata aaatcattca gaaacagaaa gagcacacgc tcgacgccgg tgtgaaatac
     5161 gcgccccata gtgacaggct ttgggt
//
LOCUS       DAJTOR010000228         5146 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTOR010000228 DAJTOR010000000
VERSION     DAJTOR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.230"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            99..2096
                     /gene="betT"
                     /locus_tag="OO249_003866"
     CDS             99..2096
                     /gene="betT"
                     /locus_tag="OO249_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR5637984.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2159..3436
                     /locus_tag="OO249_003867"
     CDS             2159..3436
                     /locus_tag="OO249_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5637985.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
     gene            complement(3716..4878)
                     /locus_tag="OO249_003868"
     CDS             complement(join(3716..4617,4617..4878))
                     /locus_tag="OO249_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HCR5637986.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYTSGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRTRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            4945..>5146
                     /locus_tag="OO249_003869"
     CDS             4945..>5146
                     /locus_tag="OO249_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5637987.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVKNANVPKGSFYYYLKSKEDY"
BASE COUNT         1214 a         1228 c         1306 g         1398 t
ORIGIN      
        1 gcattacgat taacgccgca tagccagttg aactttgcta ctttgtgaga ggtagtacct
       61 tctatccagt gcgaatttaa ttaatggaat aaatgattat gagtgaaaat gatacaatcc
      121 caaagaagtc tacaagtcag attaacaaag cggtattctt tacatctgct ttgctaattt
      181 tccttcttgt cgcctttgcc gccgtattcc cggatgtcgc cgacaaaaat tttaaactac
      241 ttcagcaaca aatcttcacg aatgccagct ggttctacat ccttgctgtg gccctgattt
      301 tactgagtgt cacgttcctt ggactctcac gctacggtga tatcaagctg ggcccggacc
      361 atgcgcagcc tgatttcagc taccactcct ggtttgcgat gcttttttcg gcagggatgg
      421 ggatcggcct gatgttcttt ggcgttgccg aacctgtaat gcattatctt tcgccacccg
      481 ttggcactcc agaaaccgtt gcggcagcta aggaagcaat gcgtctgacc tttttccact
      541 ggggactgca cgcatgggca atttatgcca ttgtggcgct gattctggcg ttcttcagtt
      601 accgtcacgg tctgccttta actctgcgct ccgcactcta tcccattatt ggcgatcgca
      661 tatacggacc tgtaggacat gcggttgata ttttcgctgt tataggcacg gtctttggcg
      721 ttgcgacatc actgggttac ggtgttttgc aggtgaatgc cggtttgaac catcttttcg
      781 gggtgcccat caatgaaacg gtgcaggtaa ttctgatcgt ggtcatcacg gggttagcga
      841 cgatttcagt ggtgtccggt ctggataagg gaatacgtat cctgtctgaa ctcaatctgg
      901 gtctggcttt gttgctcctg gcgctggtcc tgtgtctggg accaaccgtg cttctgctga
      961 agtcatttgt ggaaaatacg ggcggttatc tttcggaact ggtgagtaaa acgttcaacc
     1021 tttacgcgta tgagcccaag tcgagcaact ggctgggggg ctggacatta ctgtactggg
     1081 gatggtggct ttcatggtcg ccgtttgtgg ggatgttcat cgcacgggtc tcccgcgggc
     1141 gaaccattcg cgagtttgtc accggcgtgc tgtttgttcc cgcgggtttt acgctaatgt
     1201 ggatgacggt gtttggtaac agcgcgatct atctcattat gaaccagggg gccacagacc
     1261 tcgccaatac tgttcagcag gatgtggcgc tggccctgtt taatttcctg gagcatttcc
     1321 cgttctcttc tgtgctgtca ttcattgcaa tggcgatggt catcgtcttc tttgtaacgt
     1381 ctgctgattc gggggcaatg gttgtggata ctctggcatc aggtggagtg gcaaacacac
     1441 ccgtctggca gcgaatattc tgggcctcgc tcatgggcat tgttgcaatt gcgcttctcc
     1501 ttgccggagg gctaagtgcg ctgcaaacgg tgacaatagc gagtgcattg cccttctcag
     1561 tgatcttact aatatctata tacggacttt taaaagcttt gcgccgggat ttgaccaagc
     1621 gtgaaagcct gagcatggcg acaattgctc ctacggctgc acgtaaccca attccttggc
     1681 agagaaggtt acgcaatatc gcgtatctgc cgaagcgatc tcttgtgaaa cgttttatgg
     1741 acgacgttat ccagcctgcc atgacgctgg ttcaggagga actgaacaag caggggacga
     1801 taagccacat tagtgatgca gtcgacgatc gtattcgtct tgaagtcgat ttgggcaacg
     1861 agctgaattt tatatatgaa gtgaggcttc gcgggtatat ctcaccgacc ttcgcgctcg
     1921 ccgcaatgga taatgatgag cagcagactg aacaacatcg atattatcgc gctgaggttt
     1981 atctcaaaga aggcggtcaa aattatgatg tgatgggctg gaaccaggaa cagctgatta
     2041 atgacatact ggaccagtac gaaaaacacc tgcacttcct gcacctggtt cgttaatagc
     2101 aacatgccgt cctgggggcg gcaattatta tcccggccgc aatatgaggg aatgcagaat
     2161 gatttcacgc tggaaatgga tgctgaagca gacaattaaa aaactatggt tcagggcaac
     2221 gttattcgca attgtcgcga taataacggc ccttttatca attcttttta aatcaatgat
     2281 acctgagtcg gtttccgtga aggttggtgc ggaagcagtc gataacattc tgaacatact
     2341 ggcatcgagt atgctggcag tgaccacatt ttcgctgagt atcatggtca cagcctacgg
     2401 ttcagccact actaatgtga ctcccagagc tacgcgttta gttgttgaag acgtcaccac
     2461 acaaaatgta ctggccacct tcatcggttc ttttctcttc agtctggtag ggattattgc
     2521 cctcaatatg ggagcttatg gagaaagggg gagagtcatt ttattcattg tcacactggt
     2581 tgtcattgcc ttaatcctca tcacattgct tcgctggata cagcatttga cctctctggg
     2641 gagggttggt gagacaacgg cacaagtaga acaggcggcc atcgaaacat ttattgcgag
     2701 agcaagaaat ccctgtctcg gtggatatcc atggcttgag aacaatgaac agccgaaagg
     2761 aacggttgca gtttatccga agaagattgg ctatgttgaa tatattgata tggtgaaact
     2821 tagcaagctg ctgaccaatg atccccgtca tgtatacctc gtggcgcagc caggcagttt
     2881 catacatccg tccatgccag ttttgtacct gagtcagggc caggagtcgt caatcaacac
     2941 cgatttactt gagacgatta ttgtctcgga tgcacgttca tttgctcagg atcctcgatt
     3001 ttgtcttagc gtcatggccg aaatagcctg ccgggccctt tcccctgcag tgaacgatcc
     3061 tggaaccgcc attgatgtca ttggcagagg tgttcgtata ctttccactt acgcgcagaa
     3121 taaatctgat gaaatagaag tgaaatatcc ttcagtacat gttgcaccac ttcagaataa
     3181 cgatctactg gaagactttt tctcacctgt cgcgcgcgat ggtgccagta tgagggagat
     3241 tcagataaga gtccttaaag gactgtcgat gctgagtaaa ggttggcctg agatatttgc
     3301 tgaagccgca cataccctgg catttgaaac attagagcat gcaactcgtg ctgaccatat
     3361 agattctgat agatatctaa taaaatcaac ttattataat ttatttagtg gcgaatattc
     3421 taataaaaaa acatagctgc agagcaagca attcggttgt tggcaagtca agggcaaagc
     3481 attcgcgtga gaatgggcgt atgctttgtg ccaaaagcaa acgttgctaa ttctgcactg
     3541 ttcagcgatg gcgcagtaga gacaggcact gccactgtcc agtcggcaaa agcgaacact
     3601 ttgccactgc ggggataaac tgattgaagc tctcgcgcaa aatcacttat tcttttgcgt
     3661 taataaaatg taggagatgg gtcattgatc ctacccacgt aatatggaca caggcctaag
     3721 cgaggttctt gttttcaaat tgttccggac tgaggccgcc acaccaactg tgccgccgcc
     3781 accgattgta atcacattcg atataattaa acaccgttgc ccgcattatt tcccggctga
     3841 taaagtgttc tccatggata cattccactt tcagcgaatg aaagaagctt tccacgcagg
     3901 cattatcgta gcagcaacct tttgcgctca tacttccacg cagattatgc cgcttcagtt
     3961 gcgcctgata atctgctgaa cagtactggc ctccacggtc cgtgtgaacg ataacgttcc
     4021 ggggcctcgt acgccgccat agcgccatct gcagggcatc gcaggccagt tgcgccgtca
     4081 tgcgtggcga cattgaccag ccaataacgg cacgtgacca caggtcaatg accactgcca
     4141 gatacagcca gccttcatct gtacgtaagt acgtgatgtc tcctgcccac ttctggttcg
     4201 ggccactggt gtaaaaatcc tgctccaaca gattttctga tacaggcagg ccgtgtgcgc
     4261 ggtagctgac cgggctgaac ttccgggagg cctttgccct cagtccctga cggcgcaggc
     4321 ttgccgccac ggtttttacg ttaaaggggt aaccctgagc acgcagttca tccgtcaggc
     4381 gtggggcacc gtaacgctgt tttgaccggg taaaagccgc gaggacaacg ctgtcgcagt
     4441 gttggcggaa ctgctgacgc gtgcttatcc ttgtccgccg ctgacaccac gtataccagc
     4501 cgctgcgggc cacccggagc acgcggcaca ttgctttgat gctgaactca gcctgatgtt
     4561 tttcaataaa gacatacttc atttcaggcg cttcgcgaag tatgtcgcgg ccttttggag
     4621 gatagccagc tcttcatccc gttctgccag ctggcgtttg agacgtgcaa tctcggtaga
     4681 catctccagt tcacgttcag aagacgtctg ctgattttgc tgtttactgc gccagttgta
     4741 gagctgtgat tcatacaggc tgagttcacg ggctgcggca gtaacaccga tgcgttcagc
     4801 aagcttcagg gcttcactgc gaaattcagg cgaatgctgt ttacggggtt ttttactggt
     4861 tgatactgtt tttgtcatgt gagtcacctc tgactgagag tttactcact tagccgcgtg
     4921 tccactattg ctgggtaaga tcacatgaca ggcgaaaatc gtggccgggg aaggccgcga
     4981 aagacagaaa gtacctatgc agatactcgt aatgatctga tccgcagtgg ccttgaactg
     5041 ctgacgcaga atggttttct tgcgacggga gtcgatgcca ttgttaaaaa tgccaatgtc
     5101 cctaaaggct cattttatta ctaccttaag agtaaagagg attatg
//
LOCUS       DAJTOR010000229         4764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTOR010000229 DAJTOR010000000
VERSION     DAJTOR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.231"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..897
                     /locus_tag="OO249_003870"
     CDS             <1..897
                     /locus_tag="OO249_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCR5637988.1"
                     /translation="GPRQFTVSFDETLKPFVRDASGSRLKDLPKPNKSDDESRSNDAV
                     NRYKLLKKDARIVAAQQVARQESAMCLRRRWSPENFKLFLVEHPLVRHLTRRLIWGVY
                     SAENQLQACFRVAEDNSYSTADDDLFTLPEGGISIGIPHVLEISPTDAAAFGQLFADY
                     ELLPPFRQLDRNSYALTGAERNASELTRWAGRKCPSGRVMGLANKGWIKGTPQDGGWI
                     GWMIKPLGRWSLVMEIDEGFAVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEA
                     QFSVIDAITASELINDIEALFE"
     gene            907..1149
                     /locus_tag="OO249_003871"
     CDS             907..1149
                     /locus_tag="OO249_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5637989.1"
                     /translation="MDKELPWLADNAQLELKYKKSKTPLSHRNWPGEPVSVITESIIQ
                     TLGDELLQKAEKKKRLSGVMKIFHWSGSPPSRRPST"
     gene            2673..4538
                     /locus_tag="OO249_003872"
                     /pseudo
     CDS             2673..4538
                     /locus_tag="OO249_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1222 a         1224 c         1220 g         1098 t
ORIGIN      
        1 ggcccacgcc agttcaccgt cagctttgat gaaaccttaa aaccgtttgt gcgtgacgct
       61 tccggcagcc gcctgaaaga cctgcccaaa ccaaacaaaa gcgatgatga atcgcggtcg
      121 aacgatgcgg ttaaccgcta caaattgctg aaaaaagatg cgcgtatcgt cgccgcccag
      181 caggtagcaa ggcaggaatc cgccatgtgc ctgcgtcgcc gctggtcgcc agaaaacttt
      241 aagctcttcc tggttgagca tccgttggtt cgccacttaa cccgccgtct gatttggggc
      301 gtttatagcg ccgaaaacca gctacaggcg tgctttaggg tggcggaaga taacagctac
      361 agcaccgctg acgatgatct tttcaccctg ccggaaggcg gtatctctat cggcattcct
      421 cacgttctgg aaatatcacc gacggatgct gccgcctttg gtcagctttt tgccgactac
      481 gaactgctac caccattccg tcagcttgac cgtaacagtt acgccctgac aggagccgag
      541 cgcaacgcca gcgaactgac ccgctgggca ggcagaaaat gcccgagcgg tcgggtcatg
      601 gggctggcga ataaaggctg gataaagggc accccgcagg atggaggctg gatcggctgg
      661 atgatcaaac ccctgggccg ctggtcgtta gtcatggaaa tcgatgaagg ttttgcggta
      721 ggcatgtcgc cagccgaact cagcgctgag cagctcttaa gcaagctgtg gctatgggaa
      781 ggcaaagcag aaagctatgg ctgggggagt aattcaacac aggaagcgca gttctccgta
      841 atcgatgcca tcaccgccag cgagctaatt aacgatattg aagccctgtt tgaataagga
      901 aagagcatgg acaaggaatt accgtggctg gcggataacg cccaactgga actgaaatat
      961 aaaaaaagca aaacgccgct cagtcatcgc aattggccgg gcgaaccggt gtctgttatc
     1021 actgaaagca tcattcagac actgggtgat gaattgctac aaaaagctga gaagaaaaaa
     1081 agattgtctg gcgttatgaa aatttttcac tggagtggca gtccgccatc acgcaggcca
     1141 tcaacttgat cggcgaacac aaaccctcag tcccggcccg gacaatggcg gcgctagcct
     1201 gtatcgcgca aaatgacagc caacagttgc tcgacgaaat cgtccaacaa gaggggctgg
     1261 aatatgcgac tgaggtggta attgcacgcc tgtttattgc gcggtgttat gagagtgatc
     1321 ctctggtagt gacattgcag tatcaggacg aggattatgg ctatggttat cgctcagaaa
     1381 cctataacga attcgatctc cgactgcgta agcatctctc tctggcagag gaaagctgct
     1441 ggcagcgttg cgccgacaaa ctcattgccg cactaccagg aataaccaaa gttcgccgcc
     1501 cttttattgc gctgatcctc ccggaaaaac cagaaatagc caatgagttg gtaggccttg
     1561 aatgcccgcg aactcatttt cattctaaga agtggttaaa agttgttgct aatgacccca
     1621 cagcggtgag aaaactcgaa cactactgga gccaggatat atttagcgat cgagaagcca
     1681 gctacatgtc gcatgaaaac cacttcggct acgcggcctg cgccgccctt ttgcgcgaac
     1741 aaggactggc agccattccg cgcctcgcga tgtatgccca taaagaagat tgcggcagtc
     1801 tgctggtaca aattaaccat ccgcaagtca tccgcacctt gctactggtg gctgataaaa
     1861 acaaacccag cctgcaacgt gtagctaaat accataaaaa cttcccccat gcgacgctcg
     1921 ccgcactggc agaactgctg gcgttaacag aaccaccagc ccgccctggt tatccaatca
     1981 tcgaagacaa aaagctgcct gcacagcaaa aagcacgcga tgaatactgg cgtacgctgt
     2041 tacaaacgct gatggcatcg cagccacaac tggcagaaga ggtgatgcag tggttaagta
     2101 ctcaagccag ggcagtgctg aatagttatt tatcggcacc gcccaaaccg gttattgata
     2161 gtaccgataa cagcaatctg cctgaaatcc tcgtttcgct accgtggcgt agtaagaaaa
     2221 aaatgacagc tccacgtctt gatttggcac cgctcgaatt aactccgcaa gtttactggc
     2281 aaccaggcga acaagagagg cttgccgcca ctgagtctgc ccgttatttc agcacggaat
     2341 ctcttgcgca acgcatggaa caaaaaagtg gacgagttgt attacaggaa ctgggttttg
     2401 gggatgatgt atggctgttt ctgaattata tactccccgg aaaactggat gctgcacgca
     2461 attcactcat tgttcagtgg cattactacc aggggcgggt tgaagagatc ctgaatggct
     2521 ggaactcccc ggaagcacaa ttagcagaac aggcgctccg cagcggtcac atagaagcgt
     2581 taattaacat atgggaaaat gacaactact cacgttatcg tccggaaaag agtgtctgga
     2641 acctgtattt attggcacag ttgccgcgtg agatggcttt gaccttctgg ctgcgtatca
     2701 atgagaaaaa gcatctgttc gcgggtgagg actattttct cagtatcctc ggattggatg
     2761 cgctaccagg tctgctgttg gctttttcac atcgtccaaa agaaacattt ccgttaattt
     2821 taaattttgg cgcaacagaa ctggcgctgc ctgttgcccg cgtctggcga cgtttcgcgg
     2881 cgcagcgtga tctggctcgc cagtggattt tacaatggcc ggaacatacg gctagtgcac
     2941 ttatccctct tgtctttacc aaacccagcg ataatagcga agccgcatta cttgccctgc
     3001 gtttactgta cgaacaggga catggcgaat tgctacaaac cgtggcaaac cgctggcagc
     3061 gtacagatgt atggtctgcc ctggagcagt tgcttaaaca gggtccaatg gacatttacc
     3121 cggcacgcat tccaaaagcc cctgatttct ggcatccggc aatgtggtcc aggcctcgac
     3181 ttatcactaa taatcagcct gttaccggtg acgctctgga aattatcggc gaaatgctgc
     3241 gctttaccta ggggggacgt ttttatagcg ggctggaaca actgaaaacg ttctgccagc
     3301 cacaaacgct ggccgctttt gcctgggatc tcttcactgc ctggcaacaa gcgggggccc
     3361 ccgcaaaaga caactgggca tttctggcat tacgtctctt tggtgacgaa agcacggcac
     3421 gggatctgac gacacagatc ctcgcctggc cacaagaagg caaatctgcc cgtgctgtca
     3481 gtggtctgaa tatacttacc ctgatgaata atgatatggc gctgatgcag ctgcatcatg
     3541 tatcacaacg ggcgaaatca cgccccttac gtgaaaacgc agcggaattt cttcaggtgg
     3601 tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc ccaaccctgg
     3661 gccttgatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt accgttcgct
     3721 tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag aaaagcgttc
     3781 cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc cgactaaaag
     3841 ggctaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt gaagctgccc
     3901 tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt aatcatccct
     3961 ttacccgtct ggttacccag cgattaatat ggggggttta tccggcaaat gaaccgcgtt
     4021 gtttactcaa agcctttcgt gtggccgcag agggggagtt ctgcaatgcg caagatgagc
     4081 caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa atgacagtag
     4141 aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg ccttttcgcc
     4201 aattgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac agcctgactc
     4261 gctgggaagg taaatccgct accgttgggc agcttatggg aatgcgatac aaaggctggg
     4321 agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg gtccttaagt
     4381 tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc ttccgttctc
     4441 ttcgagtgta ccgtgacaat aaatccgtca cttttgccga acttgatgtg tttgatttga
     4501 gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa acacagcggg cagtgatgca
     4561 actgcccgtt atcaacacga cttaccagcg gggaaagcga tgatcgtgca aaaagagctg
     4621 gttgctattt acgattatga ggtccctgta cctgaaaatc cgttttcctt cagacttgag
     4681 atccataaat gctctgaatt atttacaggt tccgtctatc gactggaacg gttccggcta
     4741 cgtccaacat ttcatcaacg tgat
//
LOCUS       DAJTOR010000230         4759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTOR010000230 DAJTOR010000000
VERSION     DAJTOR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.232"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..1252)
                     /locus_tag="OO249_003873"
     CDS             complement(<1..1252)
                     /locus_tag="OO249_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGCS family amino acid carrier protein"
                     /protein_id="HCR5637990.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPRAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMA"
     gene            1531..2484
                     /gene="tal"
                     /locus_tag="OO249_003874"
     CDS             1531..2484
                     /gene="tal"
                     /locus_tag="OO249_003874"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5637991.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2599..3186
                     /gene="mog"
                     /locus_tag="OO249_003875"
     CDS             2599..3186
                     /gene="mog"
                     /locus_tag="OO249_003875"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5637992.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSVLTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(3221..3787)
                     /gene="satP"
                     /locus_tag="OO249_003876"
     CDS             complement(3221..3787)
                     /gene="satP"
                     /locus_tag="OO249_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5637993.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3936..4649)
                     /gene="msyB"
                     /locus_tag="OO249_003877"
     CDS             complement(3936..4649)
                     /gene="msyB"
                     /locus_tag="OO249_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5637994.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(4675..>4759)
                     /locus_tag="OO249_003878"
     CDS             complement(4675..>4759)
                     /locus_tag="OO249_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5637995.1"
                     /translation="RCVSKITFSGHTVNSSDMATLKIIGDD"
BASE COUNT         1258 a         1230 c         1209 g         1062 t
ORIGIN      
        1 aggccattat gatatctgcc agttgccaca tcagcggaag gcttagcaag gtgccgccga
       61 tgaccgttgc gaaggtgcag atccgcaaac accagatcgc tttaggggtg ttcaggcgta
      121 aaaagaagag attgttttcg gcataaatgt agttggcaac gatggagctg aaggcaaaca
      181 gaataaccac gagggtaaca aactcagcac cccaggaacc cattagcacc cgcatcgcct
      241 tctggataag ctgaatacct tccagcggca tgtaggttgt gtcgttaccc gccagtaata
      301 tcagcatggc gcttgccgta cagatgacca gggtgtcgat aaaaatgcca atcatctgga
      361 caatcccttg cgctgccgga tgcggaggcc aggacgccgc tgccgctgcc gcgtttggcg
      421 tcgaacccat tcccgcctca ttggaaaaca tactgcgctg aaaaccgtta gtaatcgcct
      481 ggcttaaggt atatcccgcc gcgccgcctg ccgcttcctg ccagccaaaa gcactctcaa
      541 aaatagacca aatgacgtgg ggaagttgcc cgatatttat tacgcaaatc accatgctgg
      601 tcagcaccca gattatcgcc atcaacggga caaacccctg catgagccgg gcgacgccat
      661 gaagaccgcg agtgattgcc agcagagcaa agacagcgag aataatgcct gtcaccagcg
      721 ggggaaaatc aaaagaaaaa ctcagggctc gggcaacggc gttcgcctga actccgctga
      781 aaattatgcc gtaggcgatg agcaaaaaga cggcgaacag aacgcccatc caacgcatcc
      841 ccagcccgcg cgccatatac catgccggtc cgccacgaaa ctgcccattg acgtcacgtt
      901 ctttataaag ttgtgcaagg gaacattcgg caaacgaggt cgccatgccg ataaacgcgg
      961 caacccacat ccaaaagacg gctctaggtc caccggtggt aatagccagc gcaacgccgg
     1021 ccaggttgcc gctacccacg cgcgccgcaa gactggtaca caatgactga aatgaggtta
     1081 aaccgcctgg ctgtggatga atgctatttt taagactttt gccaaactgg cggatgtagc
     1141 gaaactgcac aaatccggtg cgaaaagtga accaacaacc tgcgccgaag agcaggtaaa
     1201 tcattaccga tccccaaagg acgctgttaa taaaggagaa aaaatctggc atgcatatcc
     1261 ctcttattgc cggtcgcgat gactttcctg tgtaaacgtt accaattgtt taagaagtat
     1321 atacgctacg aggtacttga taacttctgc gtagcataca tgaggttttg tataaaaatg
     1381 gcgggcgata tcaacgcagt gtcagaaatc cgaaacagtc tcgcctggcg ataaccgtct
     1441 tgtcggcggt tgcgctgacg ttgcgtcgtg atatcatcag ggcagaccgg ttacatcccc
     1501 ctaacaagct gtttaaagag aaatactatc atgacggaca aattgacctc ccttcgtcag
     1561 tacaccaccg tagtggccga cactggggac atcgcggcaa tgaagctgta tcaaccgcag
     1621 gatgccacaa ccaacccttc tctcattctt aacgcagcgc agattccgga ataccgtaag
     1681 ttgattgatg atgctgtcgc ctgggcgaaa cagcagagca acgatcgcgc gcagcagatc
     1741 gtggacgcga ccgacaaact ggcagtaaat attggtctgg aaatcctgaa actggttccg
     1801 ggtcgtatct caactgaagt tgatgcgcgt ctttcctatg acaccgaagc gtcaattgcg
     1861 aaagcaaaac gcctgatcaa actctacaac gatgctggta ttagcaacga tcgtattctg
     1921 atcaaactgg cttctacctg gcagggtatc cgtgctgcag aacagctgga aaaagaaggc
     1981 atcaactgta acctgaccct gctgttctcc ttcgctcagg ctcgtgcttg tgcggaagca
     2041 ggcgtgttcc tgatctcgcc atttgttggc cgtattcttg actggtacaa agcgaatact
     2101 gataagaaag agtacgctcc ggcagaagat ccgggcgtgg tttctgtatc tgaaatctac
     2161 cagtactaca aagagcacgg ttatgaaacc gtggttatgg gcgcaagctt ccgtaacatc
     2221 ggcgaaattc tggaactggc aggctgcgac cgtcttacca tcgcacctac actgctgaaa
     2281 gagctggctg agagcgaagg ggctatcgaa cgtaaactgt cttacaccgg cgaagtgaaa
     2341 gcgcgtccgg cgcgtatcac tgagtccgag ttcctgtggc agcacaacca ggatccaatg
     2401 gcagtagata aactggcgga aggtatccgt aagtttgcta ttgaccagga aaaactggaa
     2461 aaaatgatcg gcgatctgct gtaatcattc ttagcgtgac cgggaagtcg gtcacgctac
     2521 ctcttctgaa gcctgtctgt cactcccttc gcagtgtatc attctgttta acgagactgt
     2581 ttaaacggaa aaatcttgat gaatacttta cgtattggct tagtttccat ctctgatcgc
     2641 gcatccagcg gcgtttatca ggataaaggc atccctgcgc tggaagaatg gctgacatcg
     2701 gtgctaacca cgccgtttga actggaaacc cgcttaatcc ccgatgagca ggcgatcatc
     2761 gagcaaacgt tgtgtgagct agtggatgaa atgagttgcc atctggtgct caccacgggc
     2821 ggaactggcc cggcgcgtcg tgacgtaacg cccgatgcga cgctggcagt agcggaccgc
     2881 gagatgcctg gctttggtga acagatgcgc cagataagcc tgcattttgt accaactgcg
     2941 atcctttcgc gtcaggttgg tgtgattcgc aaacaggcgc tgatccttaa cttacccggt
     3001 cagccgaagt ctattaaaga gacgctggaa ggtgtgaagg acgctgcggg taacgttgtg
     3061 gtacacggta tttttgccag cgtaccgtac tgcattcagt tgctggaagg gccatacgtt
     3121 gaaacggcac cagaagtggt tgctgcattc agaccgaaga gtgcaagacg cgacgttagc
     3181 gaataaaaaa atccccccga gcggggggat ctcaaaacaa ttagtgggat tcaccaatcg
     3241 gcagaacggt gcgaccaaac tgctcgttca gtacttcacc catcgccaga tagattgcac
     3301 tggcaccaca gatcagacca atccagccgg caaagtggat gattgcggcg ttaccggcaa
     3361 tgttaccgat cgccagcagg gcaaacagca cagtcaggct aaagaaaacg aattgcagaa
     3421 cgcgtgcgcc tttcagcgtg ccgaagaaca taaacagcgt aaatacgccc cacagaccca
     3481 ggtagacacc aaggaactgt gcatttggcg catcggtcag acccagtttc ggcatcagca
     3541 gaatcgcaac cagcgtcagc cagaaagaac cgtaagaggt gaatgcggtt aaaccgaaag
     3601 tgttgccttt tttgtactcc agcagaccag caaaaatttg cgcgatgccg ctgtagaaaa
     3661 tgcccatggc aagaataata ccgtccagag cgaaataacc cacgttgtgc aggttaagca
     3721 gaatggtggt catgccgaag cccatcaggc ccagcggtgc cggattagcc aacttagtgt
     3781 tgcccataat tcctcaaaaa tcatcatcga atgaatggtg aaataatttc cctgaataac
     3841 tgtagtgttt tcagggcgcg gcataataat cagccagtgg ggcagtgtct acgatctttt
     3901 gaggggaaaa tgaaaatttt ccccggtttc cggtatcaga cctgagtggc gctaaccatc
     3961 cggcgcaggc aggcgatttg cagtacggct ggaatcgtca cgcgataggc gctgccgctg
     4021 accgctttaa ccccatttag tgccgcacct acagggcctc ccagccccgc gccgcgcagc
     4081 aaaccatgcc caagtacgct cattgctgcg tgggtgcgta aaatgcgggt cagttggctg
     4141 gaaagcaaat gcgacacgcc ttttgccaat aatttgtctt tcatcagcag cggcagcagc
     4201 tcttccagct cattcaccct cgcatcgaac gcgtgcagaa actcctgctt atgttcctcg
     4261 tccattttct tccaggtatt acgcagaaat tgttccagta actgttgctc aatttcaaac
     4321 gtagacatct ctttgtcggc tttcagcttc aatcgttttg aaacatcgag caaaatggcc
     4381 cgatacaatt taccgtgtcc gcgcagtttg ttggcgatac tatcgccacc aaaatgctgt
     4441 aattctccag caatcagctg ccagttgcgg cgatgttgct cgggatgccc ttccatcgat
     4501 ttaaacagtt cgttgcgcat cagtacgctg gagaggcgag ttttgccttt ttcattatgg
     4561 gtgagcaatc gggcgaaatt tgccaactgt tcctcactac aatgctgaag aaaatccaga
     4621 tctgaatcat tcaggtaatt aacattcatt ttttgtggct tctatattct ggcgttagtc
     4681 gtcgccgata attttcagcg tggccatatc cgatgagttc accgtatgac cggaaaaggt
     4741 gatttttgag acgcagcgt
//
LOCUS       DAJTOR010000231         4672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTOR010000231 DAJTOR010000000
VERSION     DAJTOR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.233"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            227..1006
                     /gene="deoC"
                     /locus_tag="OO249_003879"
     CDS             227..1006
                     /gene="deoC"
                     /locus_tag="OO249_003879"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5637996.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            1133..2455
                     /gene="deoA"
                     /locus_tag="OO249_003880"
     CDS             1133..2455
                     /gene="deoA"
                     /locus_tag="OO249_003880"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5637997.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDARALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            2507..3730
                     /gene="deoB"
                     /locus_tag="OO249_003881"
     CDS             2507..3730
                     /gene="deoB"
                     /locus_tag="OO249_003881"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5637998.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            3810..4529
                     /gene="deoD"
                     /locus_tag="OO249_003882"
     CDS             3810..4529
                     /gene="deoD"
                     /locus_tag="OO249_003882"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5637999.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1130 a         1195 c         1282 g         1065 t
ORIGIN      
        1 ccattgtaag aagtttcgtg atgttcactt tgatcctgat gcgtttgcca ccactgacgc
       61 attcatttga aagtgaatta tttgaaccag atcgcattac agtgatgcaa acttgtaagt
      121 agatttcctt aattgtgatg tatatcgaag tgtgttgcgg agtagatgtt agaatactaa
      181 caaactcgca aggtgaattt tattggcgac aagccaggag aatgacatga ctgatctgaa
      241 agcaagcagc ctgcgtgcac tgaaattgat ggacctgacc accctgaatg acgacgacac
      301 cgacgagaaa gtaattgctc tgtgtcatca gaccaaaact ccggtcggca ataccgccgc
      361 tatctgtatc tatcctcgct ttatcccgat tgctcgcaaa acgctgaaag agcagggcac
      421 cccggaaatc cgaatcgcta cggtaaccaa cttcccacac ggtaacgatg acatcgaaat
      481 cgcgctggca gaaacccgtg cggcaatcgc ctacggtgct gatgaagttg acgttgtgtt
      541 cccgtaccgc gcgctgatgg cgggtaacga gcaggttggt tttgacctgg tgaaagcctg
      601 taaagaggct tgcgcggcag cgaatgtact gctgaaagtg atcatcgaaa ccggcgaact
      661 gaaagacgaa gcgctgatcc gtaaagcgtc tgaaatctcc atcaaagcgg gtgctgactt
      721 catcaaaacc tctaccggta aagtggcagt gaacgcgacg ccggaaagcg cgcgcatcat
      781 gatggaagtg atccgtgata tgggcgtaga aaaaaccgtt ggtttcaaac cggcgggcgg
      841 cgtgcgtact gcggaagatg cgcagaaata tctcgccatc gcagatgaac tgttcggtgc
      901 tgactgggca gatgcgcgtc actaccgctt tggtgcttcc agcctgctgg caagcctgtt
      961 gaaagcgctg ggccacggtg acggtaagag cgccagcagc tactaagcaa gatgctttac
     1021 gcctgatgcg ctgcgcttat caggcctacg agacgtatct acccgtaggc cggataaggc
     1081 gtagacgcat ccggcaaaag ccgcctcata ctcttttcct cgggaggtta ccttgtttct
     1141 cgcacaagaa attattcgta aaaaacgtga tggtcatgcg ctgagcgatg aagaaattcg
     1201 tttctttatc aacggcattc gcgacaacac tatctccgaa gggcagattg ccgccctcgc
     1261 gatgaccatt ttcttccacg atatgacaat gcctgagcgt gtctcgctga ccatggcgat
     1321 gcgagattca ggaaccgttc tcgactggaa aagcctgcat ctgaatggcc cgattgttga
     1381 taaacattcc accggcggcg tcggcgatgt cacttcgctg atgttggggc cgatggtcgc
     1441 agcctgcggc ggctatattc cgatgatctc cggtcgcggc ctcggtcata ctggcggtac
     1501 gctcgacaaa ctggaatcca tccctggctt cgacattttc ccggatgaca accgtttccg
     1561 cgaaattatt aaagacgtcg gcgtggcgat tatcggtcag accagctcac tggctccggc
     1621 ggataaacgt ttctacgcga cccgtgatat taccgcaacc gtggactcca tcccgctgat
     1681 cactgcctcg atcctggcga agaaactggc ggaaggtctg gatgcgctgg tgatggacgt
     1741 gaaagtgggt agcggcgcgt ttatgccgac ctacgaactc tctgaagccc ttgccgaagc
     1801 gattgttggc gtggctaacg gcgctggcgt gcgcaccacc gcgctgctca ccgacatgaa
     1861 tcaggtactg gcctccagtg caggtaacgc ggttgaagtt cgtgaagcgg tgcagttcct
     1921 gacgggtgaa tatcgtaacc cgcgtctgtt tgatgtcacg atggcgctgt gcgtggagat
     1981 gctgatctcc ggcaaactgg cgaaagatga cgccgaagcg cgcgcgaaat tgcaggcggt
     2041 gctggacaac ggtaaagcgg cagaagtctt tggtcgtatg gtagcggcac aaaaaggccc
     2101 gaccgacttc gttgagaact acgcgaagta tctgccgaca gcgatgctga cgaaagcagt
     2161 ctatgctgat accgaaggtt ttgtcagtga aatggatgcc cgcgcgctgg ggatggcagt
     2221 ggttgcaatg ggcggcggtc gtcgtcaggc atctgacacc atcgattaca gcgtcggctt
     2281 tactgatatg gcgcgtctgg gcgaccaagt agacggtcag cgtccgctgg cggttatcca
     2341 cgcgaaagac gaaaacagct ggcaggaagc ggcgaaagcg gtgaaagcgg caattaaact
     2401 tgccgataaa gcaccggaaa gcacaccaac tgtctatcgc cgtatcagcg aataacggta
     2461 tactgatctg atcatttaaa tttgaagcac tgagtacgga gaacatatga aacgtgcatt
     2521 tattatggtg ctggactcat tcggcatcgg cgctacagaa gatgcagaac gctttggtga
     2581 cgtcggggct gacaccctgg gtcatatcgc agaagcttgt gccaaaggcg aagctgataa
     2641 cggtcgtaaa ggcccgctca atctgccaaa tctgacccgt ctggggctgg cgaaagcaca
     2701 cgaaggttct accggtttca ttccggcggg aatggacggc aacgctgaag ttatcggcgc
     2761 gtacgcatgg gcgcacgaaa tgtcatccgg taaagatacc ccgtctggtc actgggaaat
     2821 cgccggtgtc ccggttctgt ttgagtgggg atacttctcc gatcacgaaa acagcttccc
     2881 gcaagagctg ctggataaac tggtcgaacg cgctaatctg ccgggttacc tcggtaactg
     2941 ccactcttcc ggtacggtca ttctggatca gctgggcgaa gagcacatga aaaccggcaa
     3001 gccgattttc tatacctccg ctgactccgt gttccagatt gcctgccacg aagaaacctt
     3061 tggcctggat aaactctacg aactgtgcga aatcgcccgt gaagagctga ccaacggcgg
     3121 ctacaacatc ggtcgtgtta tcgctcgtcc gtttatcggt gacaaagccg gtaacttcca
     3181 gcgtaccggt aaccgtcacg acctggctgt tgagccgcca gcaccgaccg tgctgcagaa
     3241 actggttgat gaaaaacacg gtcaggtggt ttctgtcggt aaaattgcag acatctacgc
     3301 caactgcggt atcaccaaga aagtgaaagc aactggcctg gacgcgctgt ttgacgccac
     3361 catcaaagag atgaaagaag cgggtgataa caccatcgtc ttcaccaact tcgttgactt
     3421 cgactcttcc tggggccacc gtcgcgacgt cgccggttat gctgcgggtc tggagctgtt
     3481 cgaccgccgt ctgccggagc tgatgtcttt gctgcgcgat gacgacatcc tgatcctcac
     3541 tgctgaccac ggttgtgatc cgacctggac cggtactgac cacacgcgtg aacacattcc
     3601 ggtactggtt tacggcccga aagtaaaacc gggttcactg ggtcaccgtg aaaccttcgc
     3661 ggatatcggc cagactctgg caaaatattt tggtacttct gatatggaat atggcaaagc
     3721 catgttctga taatgggtgc ggcttgatgc cctcaccccg gccctctccc acagggagag
     3781 ggtgaaaaca tgttgataag gataaaacaa tggctacccc acacattaat gcagaaatgg
     3841 gcgatttcgc tgacgtagtt ttgatgccag gcgacccgct gcgtgcgaag tatattgctg
     3901 aaactttcct tgaagatgcc cgtgaagtga acaacgttcg cggtatgctg ggcttcaccg
     3961 gtacttacaa aggccgcaaa atttccgtaa tgggtcacgg tatgggtatc ccgtcctgct
     4021 ccatctacac caaagaactg atcaccgatt tcggcgtgaa gaaaattatc cgcgtgggtt
     4081 cctgtggcgc agttctgccg cacgtaaaac tgcgcgacgt cgttatcggt atgggtgcct
     4141 gcaccgattc caaagttaac cgcatccgtt ttaaagatca tgactttgcc gctatcgctg
     4201 actttgacat ggtgcgtaac gcggtagacg cggctaaagc actgggcatt gatgctcgcg
     4261 ttggtaacct gttctccgct gacctgttct actctccgga cggcgaaatg ttcgacgtga
     4321 tggaaaaata cggcatcctc ggcgtggaaa tggaagcggc tggtatctac ggcgtcgctg
     4381 cagaatttgg cgcgaaagcc ctgaccatct gtaccgtgtc tgaccacatc cgcactcacg
     4441 agcagaccac tgccgctgag cgccagacca ccttcaacga catgatcaaa atcgcactgg
     4501 aatccgttct gctgggcgat aaagagtaat tgtgtttcgc tgaaaggcga ttgtcgtgtg
     4561 aagccggagt gggagactgc tccggctttt tagtatctat tcatcaatag caactgattt
     4621 atagatttac tattattttt catatggata gaaaataaaa tctattcaag ta
//
LOCUS       DAJTOR010000232         4655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTOR010000232 DAJTOR010000000
VERSION     DAJTOR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.234"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            50..1849
                     /gene="cysJ"
                     /locus_tag="OO249_003883"
     CDS             50..1849
                     /gene="cysJ"
                     /locus_tag="OO249_003883"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5638000.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDIEAADEFLSELRVERRYQRDVY"
     gene            1849..3561
                     /gene="cysI"
                     /locus_tag="OO249_003884"
     CDS             1849..3561
                     /gene="cysI"
                     /locus_tag="OO249_003884"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5638001.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPHHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3636..4370
                     /gene="cysH"
                     /locus_tag="OO249_003885"
     CDS             3636..4370
                     /gene="cysH"
                     /locus_tag="OO249_003885"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5638002.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
BASE COUNT         1150 a         1217 c         1328 g          960 t
ORIGIN      
        1 gcgctgacag ggcgcagaaa cagctttgct tactggaaca taacgacgca tgacgacaca
       61 ggtcccacct tccgcgttgc ttccgttgaa cccggagcaa ctggcacgcc ttcaggcggc
      121 cacgaccgat ttaacgccta cccagcttgc ctgggtttct ggctatttct ggggcgtact
      181 caatcagcag cctgctgcgc ttgcagcgac gccagcgcca gccgcagaaa tgccgggtat
      241 aactattatc tccgcctcgc aaaccggcaa tgcgcgccgg gttgctgaag cattacgcga
      301 tgatttatta gcagcaaaac tgaacgttaa gctggtgaac gcgggcgact ataaattcaa
      361 acaaatcgcc agcgaaaaac tgctcatcgt agtgacgtca acgcaagggg aaggggaacc
      421 gccggaagaa gccgtcgcgc tgcataagtt cctgttctcc aaaaaagcgc caaagctgga
      481 aaacaccgcg tttgccgtgt ttagcctcgg cgatagctct tatgaatttt tctgccagtc
      541 cgggaaagat ttcgacagca agctggcgga actgggtggt gaacgcctgc tcgaccgtgt
      601 cgatgccgat gttgaatacc aggctgctgc cagcgagtgg cgcgcccgcg tggttgatgc
      661 gcttaaatcg cgtgcgcctg tcgcggcacc ttcgcaatcc gtcgctactg gcgcggtaaa
      721 tgaaatccac accagcccgt acagcaaaga cgcgccgctg gtggctagcc tctctgttaa
      781 ccagaaaatt accgggcgta actctgaaaa agacgttcgc catatcgaaa ttgacttagg
      841 tgactcgggc ctgcgttacc agccgggtga cgcgctgggc gtctggtatc agaacgatcc
      901 ggcactggtg aaagaacttg tcgaactgct gtggctgaaa ggcgatgaac ctgtcaccgt
      961 cgagggcaaa acgttgcctc tgaacgaagc gctacagtgg cacttcgaac tgaccgtcaa
     1021 caccgccaac attgttgaga attacgccac gcttacccgc agcgaaacac tgctgccgct
     1081 ggtgggcgat aaagcgaagt tacagcatta cgccgcgacg acgccgattg tcgacatggt
     1141 gcgtttctct ccggcgcaac tggatgccga agcgctgatt aatctgctgc gcccgctgac
     1201 cccgcgcctg tattccatcg cctcctcgca ggcggaagtc gagaacgaag tacacgtcac
     1261 cgttggtgtg gtgcgttacg acgtggaagg ccgagcccgt gccggtggtg cctccagctt
     1321 cctcgcggat cgcgtggaag aagagggcga agtccgcgta tttatcgaac ataacgataa
     1381 ctttcgcctg cccgctaacc cggaaacccc ggtgattatg attggcccag gcaccggtat
     1441 tgcgccgttc cgcgccttta tgcagcaacg cgccgccgac gaagcgccag gtaaaaactg
     1501 gctgttcttt ggtaatccgc actttacgga agacttcctg taccaggtgg agtggcagcg
     1561 ctacgtcaaa gatggcgtgc tgacacgtat cgatcttgcc tggtcgcgcg accaaaaaga
     1621 aaaagtttac gtacaagaca aactgcgcga acagggcgcg gagctgtggc gctggatcaa
     1681 tgatggtgcc cacatttatg tctgcggcga cgctaatcgc atggcgaaag acgttgagca
     1741 ggcacttctg gaagtgattg ccgaatttgg tggcatggac atcgaagcgg cggatgaatt
     1801 tttaagtgag ctgcgcgtag agcgccgtta tcagcgagat gtctactaat gagcgaaaaa
     1861 catccagggc ctttagtggt cgaaggaaaa ctgacagacg ccgagcgcat gaagcttgaa
     1921 agcaactacc tgcgcggcac cattgcggaa gatttaaacg acggtctgac cggcggcttt
     1981 aagggcgaca acttcctgct gatccgcttc cacggcatgt atcagcagga tgaccgcgac
     2041 atccgcgccg aacgtgctga acagaagctg gagccgcacc acgcgatgct gcttcgctgt
     2101 cgtctgccgg gtggggtgat taccactaaa cagtggcagg cgatcgacaa atttgccggt
     2161 gaaaacacca tctatggcag cattcgcctg accaaccgcc agacattcca gttccacggc
     2221 attctgaaaa agaacgtcaa accggtgcac cagatgctgc actcggtcgg tcttgatgcg
     2281 ctggcgacag ctaacgacat gaaccgtaac gtactctgca cctcgaaccc ttacgagtcg
     2341 cagctgcacg cggaagcgta cgagtgggcg aagaagattt ctgagcatct gttgcctcgt
     2401 acccgcgcgt atgcggagat ctggctcgac caggaaaaag tcgccactac tgatgaagaa
     2461 ccgatccttg gtcagaccta cctgccgcgt aagttcaaaa ccacggtagt gatcccgccg
     2521 cagaacgata tcgatctgca tgccaacgac atgaacttcg tggcaatcgc cgaaaacggc
     2581 aagctggtag gcttcaacct gctggtgggc ggtgggcttt ctatcgaaca cggcaacaag
     2641 aaaacctacg cccgcacggc gagcgagttt ggctatctgc cgctggagca tacgctggcg
     2701 gtggccgaag ctgtcgtgac aactcagcgt gactggggta accgaaccga tcgtaaaaat
     2761 gccaaaacca aatacacgct ggagcgcgtg ggggttgaga cgtttaaagc ggaagtggag
     2821 cgtcgcgcgg ggatcaaatt tgaaccgatc cgtccatatg agttcaccgg acgaggcgat
     2881 cgtattggct gggttaaggg cattgatgat aactggcacc tgacgctgtt tatcgaaaat
     2941 ggtcgcatcc ttgattatcc ggggcgtccg ctgaaaaccg gcctgctgga gatcgcgaag
     3001 atccacaaag gcgatttccg cattacggcg aaccagaatc tgatcatcgc cggtgtaccg
     3061 gaaagcgaga aagcgaagat cgagaagatc gccaaagaga gcgggttaat gaatgccgtc
     3121 acgccgcagc gtgaaaactc gatggcctgc gtgtcattcc cgacttgccc gctggcgatg
     3181 gcggaagcag agcgtttcct gccgtctttt atcgacaaca tcgataattt aatggcgaaa
     3241 catggtgtca gcgatgagca tatcgtgatg cgtgtaacag gctgcccgaa cggttgtggt
     3301 cgcgcgatgc tggcggaagt gggcctggtg ggtaaagcgc cgggtcgcta caacctgcat
     3361 cttggcggca accgcattgg gacacgtatc ccacggatgt ataaagaaaa catcaccgag
     3421 ccggaaatcc tggcgtcgct tgatgaactg atagggcgct gggcgaaaga gcgcgaagcg
     3481 ggtgaaggct tcggcgactt tacggtgcgt gcgggcatca ttcgcccggt gctcgatccg
     3541 gcgcgcgatt tgtgggatta accatcagcc cggtcttgta ggcctgataa gaacgcgtga
     3601 gcgtcgcatc aggcaaggca aacagtgagg aatctatgtc caaactcgat ctaaacgccc
     3661 tgaacgaact gccgaaggta gatcgcattc tggcgctggc ggaaactaac gccgaactgg
     3721 aaaaactgga cgctgaaggc cgcgtagcct gggcgctgga taatctgccc ggtgaatatg
     3781 tactttcttc cagctttggc attcaggcgg cggtgagcct gcatctggtg aatcaaattc
     3841 gcccggatat tccggtgatc ctcaccgata cgggttactt gttcccggaa acctaccgct
     3901 ttattgacga gttaacggac aaactcaagc tcaacctgaa agtgtaccgt gctaccgaaa
     3961 gcgcagcctg gcaggaagca cgctacggaa aactgtggga gcagggcgtt gaaggcattg
     4021 aaaagtacaa tgacatcaac aaagtcgaac cgatgaaccg ggctctgaaa gaacttaacg
     4081 cgcaaacctg gtttgctggc ctgcgccgtg aacaatccgg cagtcgcgcc aatttaccgg
     4141 tgctggcaat tcagcgtggc gtatttaaag tgctgccgat tatcgactgg gataaccgaa
     4201 ctatttatca gtacctgcaa aaacatggcc tgaaatatca cccattatgg gatgaaggat
     4261 atttatcggt cggtgatacc catacaaccc gtaaatggga acctggcatg tcggaagaag
     4321 aaacacgttt ctttggctta aaaagggaat gtggattgca cgaagggtaa atattgccgg
     4381 ataaattaaa cgcctctgtc agaaatgatg ggggcgtttt tattttgcga gcagtattcc
     4441 tgattttaaa cgtgaacgcc gctggaagaa ttatcaggta tagaatggcg tcgggtgctt
     4501 gaggctgtct ggcgttaagc attcgcgagg ttccagatgg acaaaagccc caggcgatat
     4561 ttctatcaac ctgaggccag cgttcgaacc caaacaattc gaatgttagt ctcttctttg
     4621 cagggaggca agacatgctg acaaaatatg ccctt
//
LOCUS       DAJTOR010000233         4540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTOR010000233 DAJTOR010000000
VERSION     DAJTOR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.235"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..592
                     /locus_tag="OO249_003886"
     CDS             <1..592
                     /locus_tag="OO249_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5638003.1"
                     /translation="SAYLVGDPLEFGKKLSNAAADYFIANKIDQPKIAVINCEAFEVC
                     VQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLNAIMGESGGATL
                     GAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKMGNAVFAQTLKV
                     INKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            615..2126
                     /locus_tag="OO249_003887"
     CDS             615..2126
                     /locus_tag="OO249_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5638004.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGGRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2151..3149
                     /locus_tag="OO249_003888"
     CDS             2151..3149
                     /locus_tag="OO249_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5638005.1"
                     /translation="MSASSLSLPLGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3182..4276
                     /locus_tag="OO249_003889"
     CDS             3182..4276
                     /locus_tag="OO249_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5638006.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            4288..>4540
                     /locus_tag="OO249_003890"
     CDS             4288..>4540
                     /locus_tag="OO249_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5638007.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHG"
BASE COUNT          999 a         1074 c         1379 g         1088 t
ORIGIN      
        1 ctcggcgtat ctggtcggcg atccactgga atttggtaaa aaactgagta acgctgccgc
       61 cgattatttt attgccaata aaattgacca gccgaaaatt gccgtcatca attgcgaagc
      121 ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa gtattaaaat cccgcgttcc
      181 cggcgcgcaa attgtcgcta atcaggaagg gactgtttta gataaagcga tttccgttgg
      241 tgaaaaactg attatctcca cgccggatct caacgccatt atgggggagt cgggcggtgc
      301 gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag gccggaaaaa ttgctgtttt
      361 cggttcggat atgacaaccg aaattgctca ggagctggaa aacaatcagg tgctgaaagc
      421 ggtagtggat atttccggta agaaaatggg caatgctgtt ttcgcgcaaa cattgaaggt
      481 tatcaataaa caagccgacg gtgaaaaagt gattcaggtg ccaatcgatc tctataccaa
      541 aacggaagat ggtaaacagt ggctggcaac gcacgttgat ggtctgccct aatcgctgat
      601 tcgcagaggt ccctatgttc acggcaacag aggcagtccc ggtagcaaaa gtggtggcag
      661 gaaataagcg ttaccccggc gtcgttgcgc tggataacgt taacttcacg ctcaataaag
      721 gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa atcgactctc attcgaatgc
      781 ttaccggcag cgaacgcccg gatagcggtg atatctggat tggcgagacg cgactggaag
      841 gtgacgaagc tacgttgact cgccgtgccg ctgaactggg gggtcgtgcg gtttatcagg
      901 aattaagtct ggtggaaggg ctgacggtgg cagaaaacct ctgccttggt caatggcctc
      961 gccgcaacgg catgattgat tacctgcaaa tggcgcagga tgcccaacgt tgcttacagg
     1021 cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac gctaagcccg gcgcaaaagc
     1081 aactggtgga aattgcacgg gtgatgaagg gcgagccgcg cgtggtcatt cttgatgaac
     1141 ccaccagctc gcttgccagt gcagaagttg aactggtgat cagtgcggtg aaaaagatgt
     1201 cggcactggg cgtggcggtg atttatgtca gccaccggat ggaagaaatc cgccgcattg
     1261 cctcctgtgc caccgttatg cgcgatggtc aggtggcggg cgatgtgatg ctcgaaaaca
     1321 cctccacgca tcatattgtg tcgctaatgc tagggcgtga tcacgttgat attgcgccgg
     1381 ttgcccctca ggaaattgtg gatcaggccg tactggaagt ccgtgcgtta cgccataagc
     1441 ccaagctgga ggatatcagc tttacgttac gtcgcggcga agtgcttggc attgctggcc
     1501 tgctgggggc agggcgcagt gaattgctga aggcgattgt tgggctggag acgtatgaac
     1561 agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc cgattacggc gacatgctga
     1621 aacgcggcat tggctatacg ccagaaaacc gcaaagaagc ggggatcatt ccctggttgg
     1681 gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat cagcgccaac ggagtgctgc
     1741 aatggtccac catccgccgc ctgaccgaag aggtgatgca gcggatgacg gtcaaggccg
     1801 ccagtagcga aacgcccatc ggcacgcttt ctggtggcaa tcagcaaaaa gtggtgatcg
     1861 gtcgttgggt ctatgccgcc agccagattt tgttgctcga cgagccaacg cgcggcgtcg
     1921 atatcgaagc caaacagcag atttaccgta ttgtccgtga gctggctgcc gaaggaaaaa
     1981 gcgtggtgtt tatctccagt gaagtggagg agttgccgct ggtgtgcgac cgcatcctgt
     2041 tgttacagca cggtacgttc tcgcaggagt ttcactcgcc ggtcaatgtg gatgagctga
     2101 tgtccgccat tctgtctgtg cactgatgaa ttttaccgag gaacctgaag atgtctgctt
     2161 cgtcattatc attgccgctg ggcaagagcg tctcgctcaa acaatttgtc agtcgccata
     2221 ttaatgagat cggtttgctg gtggtaatcg ccattcttta tctggtcttc tccctgaatg
     2281 cgcctggctt tatctcattg aataaccaga tgaacgtgct tcgcgatgct gccaccatcg
     2341 ggatcgccgc ctgggcaatg acgctgatta ttatctccgg tgaaattgat gtcagcgttg
     2401 gaccgatggt ggcttttgtc tcggtgtgcc tggcattttt gctgcaattt gaagttccgt
     2461 tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct gatgggaacg ctcgccgggg
     2521 tgctgcgcgg cgtgtttaac gtgcccagtt tcgttgccac actggggctg tggagcgccc
     2581 tgcgcggaat ggggctgttt atgaccaacg cactgccagt gccgattgac gaaaacgagg
     2641 tgctggactg gctgggcgga caatttctcg gtgtgccagt atccgcgctg atcatgatgg
     2701 tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc cttcgggcgc tcggtttttg
     2761 ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat taacgttcgt cgagtgcgca
     2821 ttcttatctt taccctttcg ggattattag cggcggtgac cggcattttg ctggcggcac
     2881 gcctcggttc cggtaacgca ggtgccgcaa acggtctgga gtttgacgtc atcgccgcgg
     2941 tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc cctgttcggt acattgcttg
     3001 gtgtgctggt gattacgcta atcggtaacg gtctggtgct gctcggtatt aactcctttt
     3061 tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt gctggcgaat atcttgctga
     3121 cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc cctggcgcgg gtgcggcagg
     3181 gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa acgatgctgg cagcttattt
     3241 accaggaaat tcaaccgtcg atctgcggga agttgcggtg ccgacgccgg ggattaacca
     3301 ggtactgatc aaaatgaaat cctccgggat ttgcggaagc gatgtccact atatctatca
     3361 tcaacaccgt gccacggcgg cggcacccga taaaccgtta taccagggat ttatcaacgg
     3421 tcatgaacca tgcgggcaga ttgtggcgat ggggcaaggc tgtcgccatt ttaaagaggg
     3481 tgatcgcgtg ctggtgtatc acatttctgg ctgtggtttt tgcccgaact gccgccgcgg
     3541 ctttcctatc tcttgtactg gcaaaggaaa agcggcttac ggctggcagc gtgacggcgg
     3601 tcatgccgaa tacttgctgg cggaagaaaa agatctgatc ctcctgccgg atgcgcttag
     3661 ctacgaagat ggtgcgttta tcagttgcgg cgttggtaca gcgtatgaag gaattttgcg
     3721 cggcgaagtt tccggcagtg ataacgtgct ggtggtcggt ctggggccag tcggcatgat
     3781 ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc atcggcgttg atatgctgcc
     3841 ggaacgtctg gcgatggcaa aacagttagg ggtgatggat cacggctatt tagcaaccac
     3901 cgaaggtctg ccgcagatta tcgccgaact cacccacggt ggcgcggatg ttgcgctcga
     3961 ttgttctggt aatgccgcag gtcgcttgct ggcactgcga tccaccgctg attggggacg
     4021 ggtggtttac atcggagaaa ccggaaaagt ggagttcgag gtcagcgccg atctgatgca
     4081 ccatcaacgg cggattatcg gctcctgggt gaccagtctg ttccatatgg aaaaatgcgc
     4141 ccatgatctg acggactgga aactgtggcc gcgtaacgcc attacccatc gcttctcgct
     4201 ggaacaggct ggagatgcct atgcgctgat ggcgagcggc aaatgcggga aagttgtgat
     4261 taacttcccg gattaaggag ttagcggatg accatctata ccttatctca cggaccgcta
     4321 aagctggatg tctctgacca gggcggcgta atcgaaggct tctggcgtga cacgacgccg
     4381 cttttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg cttcctgttt tccgctggtg
     4441 ccgtttgcaa accgggtgag cggcaatcgt tttgtctggc aggggcgtga gtatcaactg
     4501 caaccgaatg ttgagtggga tgcgcactat ctacacggcg
//
LOCUS       DAJTOR010000234         4530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTOR010000234 DAJTOR010000000
VERSION     DAJTOR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.236"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(21..779)
                     /locus_tag="OO249_003891"
                     /pseudo
     CDS             complement(21..779)
                     /locus_tag="OO249_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
     gene            complement(910..2274)
                     /gene="sdaA"
                     /locus_tag="OO249_003892"
     CDS             complement(910..2274)
                     /gene="sdaA"
                     /locus_tag="OO249_003892"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5638008.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(2458..3036)
                     /locus_tag="OO249_003893"
     CDS             complement(2458..3036)
                     /locus_tag="OO249_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5638009.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVIGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(3040..4401)
                     /gene="pabB"
                     /locus_tag="OO249_003894"
     CDS             complement(3040..4401)
                     /gene="pabB"
                     /locus_tag="OO249_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5638010.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVTKILRQ
                     LEK"
     gene            4475..>4530
                     /locus_tag="OO249_003895"
     CDS             4475..>4530
                     /locus_tag="OO249_003895"
                     /inference="COORDINATES: protein motif:HMM:NF015646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5638011.1"
                     /translation="MFAGLPSLTHEQQQKAVER"
BASE COUNT         1137 a         1171 c         1164 g         1058 t
ORIGIN      
        1 ccgttaaact caggctacct caaacttagc agcaccgcca gcggtaattg tgttggaaga
       61 tagtgccatg ccagcgccgt agcccccgga ccattaacgt taatggtaaa aggaaagcgt
      121 tgcgaagaaa cctgatagtt ttcattgtct tcaggttgcg gaagggaatc taccagccca
      181 ttaccataga gcaaatgccg tttgtccacc gtcaggctgg cgctactgat ttgctgcagt
      241 tgtggctcaa ggagcattgc cgtcagtaag tcgatgttaa tcatctccat gaccccagca
      301 ttgctactgc ccgctgcagg cgtccattga atcaaaaccg gactgccttt aatcagggca
      361 cggtcgatcg tcaggcgtaa aagtggttgc ggtgcaggaa gttcagccag aatgtccacg
      421 acgggcacat tgcgataacc aaaaatgctg gagcaataga gtgtgccgtc ttgcaccagg
      481 ccaatggatc gcacagtttg gagttttgcc gcctgtttac gtaatggcaa atgggcgaca
      541 gagcagggca gaccaatcag cggaagcaag acttcgctac ctgcctgtag gggaagcagt
      601 actttatcta attcctctac agcatgattg gcgaattgta ctacccgttg ttgatttaag
      661 ttacgctgtg aaataaatcg cacgctcagg gtcgaagcga gcgtaacgag tgcgcaaatc
      721 gtacaaacaa tcattcgatt acgtcgatag gttttaatga tccgttgtgc tttttgcatg
      781 cgggccaccc tgtaagcgta tggcgacaaa cgcctgacgc aacagtggat gtgtagtggg
      841 gaatattaca gcagacgaga aagcgggtat aaattcgccc atccgttgca gatgggcgag
      901 taagaagtat tagtcacact ggactttgat tgccagacca ccgcgtgagg tttcgcggta
      961 tttggcgttc atgtccttac cggtttcgta catcgtttcg atgaccttat ccagcgagac
     1021 gcgcggtgca ctggtgcggc gcagagccat ccgtgcggcg ttaatcgcct tcacagaggc
     1081 aatggcatta cgctcaatgc acggcacctg aacctgccct gcaaccgggt cgcaggttaa
     1141 accaaggttg tgttccatgc caatttccgc cgccacgcaa acctgttccg ggctaccgcc
     1201 cagcagttct gcaagacccg cagcagccat tgaacaggca acacccactt cgccctggca
     1261 accaacttcc gcaccggaaa tagaggcgtt cattttatac aatgcaccaa tcgcgcccgc
     1321 tgccataaag taacgggtat agatgtccgg gctgaccgat tcaataaagt ggtcatagta
     1381 agccagcact gccggaacga taccgcaggc accgttggtt ggcgcagtta ccacacgacc
     1441 accggcggcg ttttcttcgt taactgccag cgcaaacatg tttacccagt caatgacatt
     1501 catcggatcg ttagacagtt tatcgctgga aaccagcatc cggcgcaggg cagacgcacg
     1561 acgtggcacg cgcagcgggc ctggcagtac accttcggtg ttcatcccgc gatcgataca
     1621 tgcctgcatg gtttgccaga catgcgcgaa atactcgtcg atctctttct tgctgtgcag
     1681 cgccagttcg ttctgcatag cgagaccaga cagcgaatag ccggtttcat tacagtacgc
     1741 gagcagttcg gtggcagatt tgaacggata cggcacgctc acttcgttgg cagcatcctg
     1801 accaaagtgt tcttcatcga cgataaaacc gccgccgatg gaataataag ttttgctgta
     1861 gacgacttca tcgccgttat aggcgtggat ttgcataccg ttttcatgca gcggcaggtt
     1921 gccgttatga aaacgcatcc cgttgtcgcg cgggaaatcc acttcatgcc gtccctgtgc
     1981 cagcagcaga cgttcgcgct cttctacgtc gcgaataaaa ccgggaatac tgtcgatatc
     2041 cacggtggca ggttcgttac ctgcaagacc cataataatg gcgatatcgg tgtggtggcc
     2101 tttacccgtc agcgacagtg aaccataaac gtccacggca acgcgagtaa cgctatccag
     2161 taagcctttt tcgaccagat catcgacgaa ctgtttaccc gccttcatag gccctacggt
     2221 atgggaagat gagggaccaa tccccacctt aaacatgtcg aatagactaa tcacgataat
     2281 actcctgaca aggtgactgg acttccagta acgaactaat aacagcgcat agtgtaagag
     2341 ggaacgcggc gactggctta actattcaca tgaattaaac taatcaaaaa ccgcgattta
     2401 actaatattg cgatgattgt ctcaagtagt gttttgtaga tgtaaagata agtatagtca
     2461 gggtttcaca ccaatttgca gcgccagctc acgaattatg cctgcggtca ttccccatac
     2521 aaaatactgt tcgtaccagg acagccatac ccgatgtgaa tcgccacggc ggtagatatc
     2581 taaagggtga taacgaccca gatgtaatgc ctgggcgagc ggcatttcaa acaccgccga
     2641 gacttcatct tcactggcgc gatacggcag atcgggcggg ataatgccga tcactggggt
     2701 tacctgatag ccagttacgc tatcgacggg cggcagcacg ccgataactt caacggcgga
     2761 aggcggtata gcgacctctt cttcagcttc gcgcagcgcg gcggcgataa ctgatgcgtc
     2821 cgtgtcatcg actgcacctc cagggaatgc cacttgtcca gcgtgtttac gcagatgaat
     2881 tgaacgctga gtcagcaaca accccggttg cggtcgacgg acgatgggga ttaacacggc
     2941 agcctgacga tgatttaggg tttcccggtt aatttgcggg cgcaaaagtt gaaagcgaga
     3001 taaaaaatca tcaagcgtca ggctacggta ttccacgtct tacttctcca gttggcgtaa
     3061 tatcttagta actttatcaa aagtttcctg atattccgct tcttcctggc tatcggcgac
     3121 aattccaccg cccgcagagc agtatatttg tccgttaatg gcagtcagcg tgcggatagt
     3181 aatgctggta tccatgttgc cgcaaaagct caaatagcca atgctgccgc accaggcatt
     3241 acgtcgctgc ggttccagtt cgtcgataat ttccatagcc cgtactttcg gagccccggt
     3301 tattgagcca ccaggaaaag ctgcgcgcag cagatcgctg gcgtgtaact gttctggtag
     3361 tcgcgccgtt atagtgctga ccagatgatg cacggcaggg aagggttcca ccacgaagag
     3421 ctctggtact tttaccgaac cggctacggc aacacgaccg atatcattac gcattaaatc
     3481 gacaatcatc agattttcgg cacgatcttt cgctgagttc gccagttttt ctgcttgttt
     3541 gctatcttcc tgaggatcgg gcaggcgtgg tagcgtgcct ttaatcgggc gggtctggat
     3601 ttcactatta tcacaaagaa taaaccgctc tggcgaaagg cttaaaattg caccctgttc
     3661 aagacgtaaa aaagcgctaa atggcgcgcg gttggcctga ttaagctgaa ggaatgcctg
     3721 ccattcatcg ccagaatagg tcgcatgaaa acgctgggcg agattcacct gatagcaatc
     3781 accgctgtgc agatattcct gtacctggcg aaatttttcg ccgtactgct cgcgggtcat
     3841 attggattgc cagtcggaag tgagcgtgaa atcttcctgc ggcgagaatt gctggctttc
     3901 cagccaggcc cgacgggcat tgacatcatt atgactcagc aaagaaactg tatgacgctg
     3961 gtggtcgaca atgagcgccc aatcgtagat acccactgcc atatccggca gaacgatatc
     4021 ttgttccgca atttctggca gtgactcaaa acggcggccc agatcgtagc caaacaaccc
     4081 cagtgcgccg ccctgaaatg gcaaatcttc gttatgcgtt gggcgaatgt ctgcgcgatc
     4141 cagcacctgc tggagcacct gtagcgggtc atcagtggtc gttgtgcgtt tttcgctttc
     4201 actaacaacg gtttctttac cgaaagtggt taaagtgcaa atcggctcgg cgaccacaat
     4261 atcaaagcgg ctatacggat gatcggcata gccggagtgt aaaagcatcg cccacggcag
     4321 gtggcttaag cgggagaaat aaaattcagc ggcgtcctga cgccagggta aagtaatcac
     4381 agcgggagat aacgtcttca tcagtcctga ctctactggc tatgtgccgg gtagcatgaa
     4441 ataattagcg ccaacaattt agcaggagtt aacaatgttt gcaggtttac cttcactcac
     4501 ccatgaacag cagcaaaaag ctgtcgagcg
//
LOCUS       DAJTOR010000235         4490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTOR010000235 DAJTOR010000000
VERSION     DAJTOR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.237"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..750)
                     /locus_tag="OO249_003896"
     CDS             complement(<1..750)
                     /locus_tag="OO249_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5638012.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWFNSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIPSAVMAACLWQLPGIRTH"
     gene            complement(743..3022)
                     /locus_tag="OO249_003897"
     CDS             complement(743..3022)
                     /locus_tag="OO249_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638013.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQIALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQRNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLANAVTGCYLTPYSEKHLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASIADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DANFINALPDLRAVMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(3033..4121)
                     /locus_tag="OO249_003898"
     CDS             complement(3033..4121)
                     /locus_tag="OO249_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5638014.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1040 a         1203 c         1223 g         1024 t
ORIGIN      
        1 atgggtacga atgccgggta actgccacaa acaggccgcc atcaccgcag aggggatcac
       61 cgaatcgacc atcgagccgc tttgatcaac cagtaagacc agttgccatt gttcgctttg
      121 gcgtttaatg cggctgttaa agcgggggga ttcgatatac aacttgccgt gttgcgggtg
      181 ccagtgttgc aggttggcgc gcagagtact tttgaaatca aagtttcgcg ccagtggaat
      241 aaatgagcgg cgacggcgat cgcggacacc agaaaaagcc tgacgaactt cctttgccag
      301 tcgcgccata atttcttcaa caacctggcg cacaatcctg cgggcggcag ccagtacttc
      361 ggggttcatc agatgtttgg tgtgcaaaac ggcgcgtagc aggctttcgg aaggctgcat
      421 acgttccagc acgtcgagat ttgtcaccac atcttcaatg ccgtagcgca gtacggcatc
      481 gctttccagc cgctcaatca cctgttgcgg aaacagcgtg tggatactgt tgaaccactc
      541 aggagtggtg agatttgagc cacctaatcc accagagcgt tcaccacgct ggagccgttc
      601 aggatcgcgc ccatacagcc actccagcgc gtgatctatc tgccgggcgt tgtcatccag
      661 cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat cgccagcgtt gtagctcacg
      721 ggtggtcaga agatcgttca gttcagacat ggaaaactcc ccagtgttgc agcgatgcca
      781 gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg cggtggacaa tgcaacggca
      841 tttgcagtgc cgaaacggga agttgcgcca gttgataatg ctcaagcacc tgatgcgcca
      901 gcgtcccgcg ttctcgtggc ggtagccagg ccatcaccgc gcgtaaatcg ggcagggcat
      961 tgataaaatt ggcatcactc agttgattca aatggctgct gaaaccggcg ataaatgcag
     1021 gttgacaggc cagttgatga cgggctaatg ccagcaaacc gtgcagcgcc tcgccggatt
     1081 gtgccgggga taactgcgcc agcatcgtca gcgccgcttc ggcactggcg ttgggatgtt
     1141 ccagacgcat tagtgcgcca agagccgcgc cgcgatccag cgccggggca tgaatcgcct
     1201 gactgcgtcg ctccagtaag gctaccgccg cagaaagcga aacgccggat aaattaacgc
     1261 cgctatgtag atcgcgcaga atatggcaaa gcgcttgcca gctatgcagg tgagcgtgaa
     1321 actccttttc atccggtctg ccgttagatt cacacagcca cagcgtgcga tcgatagccg
     1381 cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc gctaatttca tccagccgcc
     1441 ataaggcata tagcacttcc agcgcctggc ccatttcggc aaattgattt tcctgggcga
     1501 ttaataatgt gagttgctcc agtaattgtt gactgaagga cgccagaccc gctaacgccg
     1561 cctggctaag acatgtggtg atactgccga tgccacccgc gtccagcata tcggcttcta
     1621 atttatggcg tgcggcttcc tgaagtgttg caccaaagca tgcggcctca atcaatgccg
     1681 catgctggct aagcgggcgg gttaatttcc agtgttcttc accgttgcct gcaagtgtca
     1741 gtgtacttcc ctgctggcgt acaatcccag ggatctccag tattgccagc cgatgtaaca
     1801 cctgactttg cgccagccca tcgggggtaa agcgatttaa ggtaagctcc gccggaagag
     1861 agattgcggt acgttccagt tcgcaggtga catcttttgg cagaggcggt tgtggcgtgg
     1921 aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc tatcaacgtt agcagaatcg
     1981 gatcggtatc gggatgaata acgccgcgat agctccacgg caacggtgca ttcagggctt
     2041 ctttaatcag cgagcctgct atggcatcca gccagtcagt gcgtaacggt agtgtatgac
     2101 cgcgcaactg tgccagtgcc attgcatgca gatgtgcggc agccatatcc gcgatcgaag
     2161 caggcaagtt gtgctggcgc aaacgggtga gaaccgtttt tagcagttgt tcaccggcct
     2221 gctgtaagcc ccactgccag caccagtttt gccagaccgg ggcaggcata ccggaaaggt
     2281 atcctgccag cacatcaagg tgcttttcac tgtagggggt gagataacaa cctgtaacgg
     2341 catttgccag cgagggcaat tctggcttgt taatttcctg cggacattcg cgccacattt
     2401 ttgccagtgc aggagcgtgc cagccaccgc agacgactaa cacgtcgcca ttatttcgct
     2461 gcaccgccca tgcaatccag cgagccataa aggcttcgcg ctgacgattg agtgcatcgc
     2521 cggggaaatc gccccgcagt tgggcaaaat agtgcgccag cgcagagggt aatgcaattt
     2581 gctggctttc atcttcgaac aagtgatccc acagggtgtc gctgttatcc atgcgggtgg
     2641 cacgcagcag caaggcctgg ctttcctctt gcgtatcagg cgaatcgtcc acttcttcac
     2701 tctgcgccca gcaaggcaaa tcgatgaagt aagtttgtgc ctgaatacga cgtgcggctt
     2761 gtagcgcctg ccactccggc gaaaattcag caaatggcgt ccaggcacca cgcccggggg
     2821 ctgcaccgtc ctgatactgg caataactgt aaatagctac cggaagctgg tgggctaaaa
     2881 acagttcgtc tacccggtca ttaaaatcag ccgggccttc aatcaatacg tatcgtggcc
     2941 gctggctttc gattaacgat ttcaccagcc gggcgcaggc cggactatga tgccggatgc
     3001 cgacaattaa cggctcgctc atgcatcttt cctcacggca ggcggtggcg ggcttgataa
     3061 taagcctgcc agtgagcttc tttatgtgtg gcgactcgtt gttcgaagta acggcgcaga
     3121 cgagcgcgat cttcttcgtt atctttgacg atggttccgg caatgcagtc caccaggtcg
     3181 gctggctcac ctgcgcgatt cgctaaaaac caggcgcgga cgcctacggc gtgggccacg
     3241 ttcacggctt cagcggtgga cataatcgct gttagtgtat ccattgacgt tttcttttcg
     3301 ccgttggcac gcagatcgcg gaaggtgcgg accagtaact ccagtaccgc gtcaggcact
     3361 ttatgcggaa taccgctatg agccagcaaa cgcgccgaag cactggcgac cagttccagc
     3421 tcctgggcga aatccataat cggaaagaca gtttcaaaat cgaagcgacg ctttagcgca
     3481 gcactcattt cattgacgcc gcgatcgcgg gtattggcag tggcaataat attaaagcct
     3541 tctcgcgcat agagctgact ggcttcacca gtgagttccg gcaccgtcat caccctgtca
     3601 gaaagcatac ctaacagaca gtcttgcact tccagcggtg tacgcgttat ctcttcaaaa
     3661 cggacgattt tgccgtcgcg cattccctgg taaagcgggg cagggaccag cgcttccgtt
     3721 gatggaccat gattgataag cagggcgtag ttccagccgt acttgatttg atcttcggta
     3781 gtagatgcgc cgccctggat ggttaacccg gcatcgccgc taatagcggt tgccagtaat
     3841 tcagacagga gagattttgc ggtgccaggt tcccccacca acatcaaacc gcgtccggtg
     3901 gcaagagtga ctaacatacg ttcgacagcg gagggactaa tcaccacttt acggctaata
     3961 ttttgcgcgc tatcgccaag gataaaagca cgggccgcag gcaaacttaa ctgccaaccg
     4021 ggcgggcaag gtgcgttatc attttgtttt aattttgcca gttcatcggc gtataacaca
     4081 gcagcaggcg gacgctgcag atgattattc tgcggtgaca tatattcatc cctgaaatgg
     4141 attaatgaaa taaacgatat tttacgcgca ataaatgttt ggcgcattag ttgtttgagc
     4201 gaaaaatatc tggaaagcag atcacagtaa ataaaaaaac ctgctggtga ggcaggttct
     4261 tttatttact actttcaggc ctaacaccac agcctgtgca acatcgtgtt ccaacgaacg
     4321 aattataacc acggctggac cccgttgcaa ttaagataat gagatttata atccagaccg
     4381 ttgggacgtt gcgccggatc gaatctattt caagtcctgg ctaccggacg ggtggtgctg
     4441 caggtggtgc cctgatccgg agtgcaacat cgtggtccaa catacgtaaa
//
LOCUS       DAJTOR010000236         4385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTOR010000236 DAJTOR010000000
VERSION     DAJTOR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.238"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            117..446
                     /locus_tag="OO249_003899"
     CDS             117..446
                     /locus_tag="OO249_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5638015.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            694..939
                     /gene="nrdH"
                     /locus_tag="OO249_003900"
     CDS             694..939
                     /gene="nrdH"
                     /locus_tag="OO249_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5638016.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            936..1346
                     /gene="nrdI"
                     /locus_tag="OO249_003901"
     CDS             936..1346
                     /gene="nrdI"
                     /locus_tag="OO249_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5638017.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1319..3463
                     /gene="nrdE"
                     /locus_tag="OO249_003902"
     CDS             1319..3463
                     /gene="nrdE"
                     /locus_tag="OO249_003902"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5638018.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENVQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3473..>4385
                     /gene="nrdF"
                     /locus_tag="OO249_003903"
     CDS             3473..>4385
                     /gene="nrdF"
                     /locus_tag="OO249_003903"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5638019.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMAKISLGQREEL
                     KSFAFDLLLELYDNELQYSDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSRIGN"
BASE COUNT         1080 a         1116 c         1123 g         1066 t
ORIGIN      
        1 ttacctgatt gcgcattcac aacgctttca gaaaagtcca taaagccgcg atgaacctca
       61 gttaagaaaa ttatggtcta cactgaaaat tacatcgaat tctaatgggg gatcatatgt
      121 ttaaccgacc gaaccgcaac gacgttgatg atggcgtgca ggatatccag aatgatgtca
      181 atcaattagc tgacagcctc gaatctgtat tgaagtcctg gggcagcgac gccaaagggg
      241 aagctgaagc cgcacgcagt aaagctcagg cgttgctgaa agaaaccagg gcgcgaatgc
      301 atggtcgtac tcgcgtccag caagccgcgc gcgatgccgt tggctgcgcg gattcttttg
      361 ttcgtgaaag accctggtgt agcgtgggta cagcagctgc ggtaggtatt tttatcggcg
      421 cactgttaag catgcgcaaa tcgtagtgca aaaatgataa taaatacgcg tctttgaccc
      481 cgaagcctgt cttcggggtt tctttttgcc tggtgaatca caaaaatccc cctcccccgt
      541 cacgctcata tccagggtaa tttcgatcac tatttgctat atattgtgta gttgaatctt
      601 ttttcaacta catctagtgt ctctgtatca acagagagac aacccgacgc gtatcatcgc
      661 gccgtatctt cattttaaac ggaaatacga atcatgcgca taactattta cactcgtaac
      721 gattgcgttc agtgccacgc caccaaacgg gcgatggaaa accgggggtt tgattttgaa
      781 atgattaatg tcgatcgcgt tcctgaagca gcggaagcgt tgcgtgctca gggctttcgt
      841 cagttgccgg tagtgattgc tggcgatctt agctggtctg gtttccgtcc ggacatgatt
      901 aaccgtctgc atccagcgcc acacgcggcc agtgcatgag ccagctcgtc tacttctcca
      961 gcagctccga aaacacgcag cgttttatcg aacgtttagg tctgcccgcg gtgcgcatcc
     1021 cgctcaatga gcgggaacgg attcaggtag acgagcctta catcctgatc gtgccctctt
     1081 acggcggcgg cggtacggct ggcgcggtgc cacgacaggt aattcgcttt ttaaacgacg
     1141 agcacaaccg ggcgttgctt cgcggcgtta ttgcttctgg taatcgcaac tttggtgagg
     1201 cgtatggccg cgccggagat gtgattgccc ggaaatgcgg cgtgccgtgg ctgtaccgtt
     1261 ttgaactcat gggtacgcaa agcgatatcg aaaacgttcg taaaggagta accgaatttt
     1321 ggcaacgaca accgcagaat gcctgacgca ggaaacgatg gattaccacg cgctgaatgc
     1381 gatgcttaac ctctacgata gcgcaggtcg cattcagttc gataaagacc gccaggccgt
     1441 tgacgccttt atggcgacgc atgtgcgtcc gaacagtgtg accttcagta gccagcagca
     1501 gcgcctgaac tggctggtca acgaaggtta ctatgatgaa agcgttctta atcgctactc
     1561 tcgcgacttt gtcattacgc tgtttgccta cgcacacacc agcggttttc gtttccagac
     1621 attcctcggg gcatggaagt tttacaccag ctatacgttg aagacattcg acggtaaacg
     1681 ttatctggaa gattttgccg atcgagtcac gatggtggcg ctgacgctgg cacaaggcga
     1741 tgcgacgctg gcgttgcaac tgaccgatga aatgctgtca ggacgctttc agccagccac
     1801 gccgacattc ctcaactgcg gtaagcagca gcgcggcgaa ctggtttcct gttttctgct
     1861 gcgtattgaa gacaatatgg agtcgattgg ccgggcggta aattccgcac tgcaactgtc
     1921 gaaacgcggc ggcggcgtgg catttttgct gtcgaatctg cgagaagcgg gcgcgccaat
     1981 taaacgtatt gaaaatcaat cttctggcgt aattccggtg atgaaaatgc tggaagacgc
     2041 attttcctat gccaaccaac tcggcgcgcg tcagggggca ggtgcagtct atttacatgc
     2101 tcatcatccc gatattctgc gttttctcga tacaaaacgg gaaaatgccg acgaaaaaat
     2161 ccgcattaaa acactgtcgc tgggcgtggt gatcccggat atcactttcc atctggcaaa
     2221 agagaatgtg cagatggcgc tgttttcgcc ctatgacgtt gagcgagttt atggcaagcc
     2281 gtttgccgat atcgccatca gcgaacacta tgacgaactg gttgccgatg aacgcatccg
     2341 caaaaaatac ctcaacgccc gtgatttctt ccagcggctg gcagaaatcc agtttgagtc
     2401 cggctatccc tacatcatgt atgaagacac ggtaaaccgt gctaaccctg tcgccgggcg
     2461 cataaatatg agtaatctct gctcagagat tttgcaggtc aactgtgcct cagagtatga
     2521 cgagaatctc gactatgccc gcacaggcca tgatatctcc tgcaatttag gttcgttgaa
     2581 tattgcgcac accatggatt cccccgattt tgcccgcacg gtagagactg ccgtgcgcgg
     2641 tttaacggcg gtgtcagata tgagtcatat ccgcagcgtg ccgtccatcg aagccggaaa
     2701 tgccgcctca cacgccatcg gactggggca gatgaattta cacggctatc tggcgcgaga
     2761 aggcatcgct tatggttcgc cggaagcact ggatttcacc aatctctatt tctatgccat
     2821 cacctggcac gcactgcgta cctcgatgtt gctggcgcgc gaacgcggtg aaaccttcgc
     2881 cgggttcaaa cagtcacgtt atgccagtgg tgaatatttt agccaatatc tgcaaggtaa
     2941 ctggcagccg aaaacggcga aagttggcga actgtttgcc cgcagcggta ttacgttacc
     3001 tacccgtgag atgtgggcgc aactgcgcga cgacgtgatg cgctacggca tttataacca
     3061 gaatcttcag gcggtgccgc caaccggttc tatctcttat atcaaccatg ctacgtcgag
     3121 tattcatccg attgtggcga aagtagagat acgcaaagag ggcaaaacag gacgcgttta
     3181 ctaccctgcc ccgtttatga ctaacgagaa tctggcgctg tatcaggatg cttacgaaat
     3241 cggcgcagaa aagatcatcg acacctacgc cgaagcgact cgccatgtcg atcaggggct
     3301 gtcgctgacg ctgtttttcc ccgataccgc caccactcgc gatatcaaca aagcgcagat
     3361 ttacgcctgg cgcaagggta tcaaaacgct ctattacatt cgcctgcgtc agatggcgct
     3421 ggaaggcact gaaattgaag gctgcgtctc ctgtgcactt taaggaatat ctatgaaact
     3481 ctcacgtatc agcgccatca actggaacaa gatatctgac gataaagatc tggaggtgtg
     3541 gaatcgcctg accagcaatt tctggctacc agaaaaggtg ccgctgtcga acgatattcc
     3601 tgcctggcag acattaactg tcgtagaaca acaactgacg atgcgcgttt ttactggcct
     3661 gacgctgctc gacacgctgc aaaatgttat cggcgcgcct tcgctgatgc cctatgcact
     3721 cacgcctcat gaagaagcgg tattatcgaa tatcagcttt atggaagcgg ttcatgcccg
     3781 ctcttacagt tcgattttct cgacgctgtg tcagaccaaa gatgtcgatg ccgcctacgc
     3841 ctggagtgaa gaaaacgcac cgttgcagcg aaaagctcag attattcagc aacattatcg
     3901 cggtgatgat ccgctgaaaa agaaaatcgc cagtgtgttt cttgaatctt ttttgttcta
     3961 ttccggtttc tggctgccga tgtatttttc cagccgcgga aagctgacca ataccgcaga
     4021 tctgatccgt ctgattatcc gcgatgaagc agtccacggt tactacatag gctataaata
     4081 tcagaaaaac atggcaaaga tatctctggg acaacgtgaa gagttgaaga gtttcgcctt
     4141 cgatttgttg ctggaactct acgacaacga gttgcaatac agcgatgagc tgtacgccga
     4201 aaccccgtgg gctgacgatg tgaaagcgtt tctctgttac aacgccaata aggctttgat
     4261 gaatctgggc tacgaaccgt tatttcccgc agaaatggcg gaagtgaatc cggcaatcct
     4321 cgccgcgctt tcgccgaatg ccgatgaaaa tcacgatttc ttttccggtt cacgtatagg
     4381 aaatt
//
LOCUS       DAJTOR010000237         4336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTOR010000237 DAJTOR010000000
VERSION     DAJTOR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.239"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            253..615
                     /gene="queD"
                     /locus_tag="OO249_003904"
     CDS             253..615
                     /gene="queD"
                     /locus_tag="OO249_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5638020.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            693..1961
                     /locus_tag="OO249_003905"
     CDS             693..1961
                     /locus_tag="OO249_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5638021.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMGMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            1952..2212
                     /locus_tag="OO249_003906"
     CDS             1952..2212
                     /locus_tag="OO249_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5638022.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFSITYRFG"
     gene            2229..2804
                     /locus_tag="OO249_003907"
     CDS             2229..2804
                     /locus_tag="OO249_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR5638023.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(2952..3812)
                     /locus_tag="OO249_003908"
     CDS             complement(2952..3812)
                     /locus_tag="OO249_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5638024.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGIQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(3809..>4336)
                     /locus_tag="OO249_003909"
     CDS             complement(3809..>4336)
                     /locus_tag="OO249_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5638025.1"
                     /translation="FEEAVLLETAADLRFAPEFVARHIAEWQHQNPLDLIITGSQSSE
                     GQNGQTPFLLAEMLGWTCFTQVERFTLDALFITLEQRTEHGLRCCRVRLPAVIAVRQC
                     GEVALPVPGMRQRMAAGKAEIIRKTVAAEMPAMQCLQLARAEQRRGATLIDGQTVAEK
                     AQKLWQDYLRQRMQP"
BASE COUNT         1001 a         1151 c         1108 g         1076 t
ORIGIN      
        1 agataaggtt aacggggtga acggtgtgga ttaaagacgg gatagcgata actaataacc
       61 aaatcgacta acctgtttta gcaatagtct ttaacaacaa aatagattaa ccaacccaat
      121 gaaaaacaaa tgaatttagc caatcattaa gataaatcag cgattttgcg caacaaagtc
      181 gttttagata atgcgaaaaa acagcctttc cggtactcta cggcggtttt atcgtcctgt
      241 agagaaatta tgatgtccac cacgttattt aaagatttca ccttcgaagc cgctcaccgc
      301 ttaccacacg tcccggaagg gcataaatgt ggtcgcctgc acgggcattc ctttatggtg
      361 cgactggaaa ttaccgggga agtcgatccg catacgggct ggattatcga tttcgctgaa
      421 ctaaaagcgg cgtttaagcc aacttatgag cgcctcgatc accattatct caatgatatt
      481 ccaggtctgg aaaacccaac tagcgaagtt ttagcaaaat ggatttggga tcaggttaaa
      541 cccgttgtgc cgctgttaag tgcggtgatg gtaaaagaaa cctgcaccgc tggttgtatc
      601 tatcgcggcg aatgataaga gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta
      661 gggtattgtg ttcaggatat ctggggcatg acatggaaga cgactgcgac attattatta
      721 ttggtgccgg tattgcaggc accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg
      781 ttttgttact ggaacgcgct gaaatccccg gcagcaaaaa tctttccggc ggacggttat
      841 atacccatgc actcgcggaa ctcctccctc agtttcatct gaccgcgcct cttgaacgac
      901 gcatcactca cgaaagcctt tccctgttaa cgcccgatgg cgtaacgacg ttttccagct
      961 tacagcccgg cggtgaatcc tggagtgtat tacgtgcacg gttcgatccg tggctggttg
     1021 ccgaagccga aaaagaaggt gtcgaatgta tccccggagc gacggtggat gcactgtatg
     1081 aagaaaacgg cagagtctgt ggcgttattt gtggtgacga tattctccgc gcccgttatg
     1141 tggtgctggc agaaggtgcc aacagcgtcc tggctgaacg tcacgggtta gtgactcgtc
     1201 ctgctggcga agcgatggcg ttggggatca aagaagtgct gtcgctggaa acatccgcta
     1261 ttgaagaacg ttttcatctg gagaataacg aaggcgcagc gttgctgttc agcggcggaa
     1321 tctgtgatga cttacccggc ggcgcatttc tttatactaa tcaacaaacg ctctcgttag
     1381 ggattgtttg cccgctctct tcccttacgc aaagtcgtgt tccggcaagc gagctgctga
     1441 ctcgctttaa agcgcatccg gcagtgcgcc cgcttatcaa aaacacggaa tcactggagt
     1501 atggtgcgca tctggtgcca gaaggtggct tgcacagtat gccggtgcaa tacgccggta
     1561 acggctggca gctggtgggc gatgcgttgc gcagttgcgt caataccgga atttccgtgc
     1621 gcggcatggg tatggcgctg actggcgcgc aggcggcaca aacgctgata agcgcctgcc
     1681 agcaccgcga gccgcaaaat ctgtttgcgc tttatcatca caacgtagag cgcagcctgc
     1741 tatgggatgt tctacaacgt tatcagcatg ttccggcgct tttgcaacgc cctggctggt
     1801 atcgggcgtg gcctgcgtta atgcaggata tttcccgcga tttatgggat cagggtgata
     1861 aacctgttcc accgctgcgc cagttattct ggcgtcattt acgtcgtcat ggcctgtggc
     1921 atctggcggg cgatgttatc aggagtctgc gatgtctgta gcccataatc tctggcgcgt
     1981 tgctgatgcg ccgcacattg ttccggctga ctccgttgag cgtcagatgg tacaatggtt
     2041 gattaacgcc tgtccggcag gtcttttttc gctcacaccg gaaggtgact tacgcgttga
     2101 ttatcacggc tgcctggagt gtggcacctg ccgtttgctg tgcgacgaat ccacactaca
     2161 acagtggcgc tatccgcctt ccggattcag catcacctac cgctttggat aaataataag
     2221 gataatttat gcccctctta cacctgctcc gccagaatcc ggtgattgct gccgttaaag
     2281 ataatgccag cctgcaactg gcaatcgact ctgaatgcca gtttatttcc gtgttgtacg
     2341 gcaatatctg caccatcagt aatatcgtta aaaagattaa gaacgccggg aaatatgctt
     2401 ttattcatgt tgatttgctg gaaggcgcgt caaataaaga agtcgtgatt cagtttttga
     2461 aactggtgac cgaggcggac ggcattatca gtaccaaagc ctcaatgttg aaagcggcaa
     2521 gagcggaagg ttttttctgt attcatcgcc tgtttattgt tgattcaatt tcgtttcaca
     2581 acattgataa gcaagttgcg caatcgaatc cggattgtat tgagatcctg ccaggctgta
     2641 tgcccaaagt gctgggctgg gtgacagaga aaatccgcca accgctgatt gccggtgggc
     2701 tggtgtgcga tgaagaagat gcgtgtaatg cgattaacgc gggtgtcgtg gcgctttcca
     2761 ccacgaatac cggggtctgg acgttagcga aaaaattact ttgacgggat aatcgtaacc
     2821 aattgaattt ggtttgattt gtaggccgca cgccacatcc gacattcagc gcctgatgcg
     2881 acgcttgacg cgtcttatca ggcctacaag tccccaggcc agaaccgtag gcaggatcat
     2941 ccggcaacac attattggca atctgcgtga atgtttgtca ccagtgcctc cagcaccact
     3001 ttccagtcat caaccacgcc aacatctgcc tgtgaaaata ccgcagcgct ggcgtcatga
     3061 ttaatcgcca ccacaaactt gctattgcgt acgcccgcca ttaacgctgc tgcgccggaa
     3121 gcgccaacaa caatacacac ctcaggtgcc agcaaatgtc cggaaatacc aatcactttt
     3181 tcagcatcaa cgccaccgtt catcactcgc gcccgactgt agcccacttc ggctcccagc
     3241 ttttctgcca gcatagcgat ttcctggtta tctgcctctc cgccctgccc caccaccagc
     3301 acccgtctgg cctcagctaa tgggtcgcga gtaacatttt tcagatcctc tgtgctaacc
     3361 agccagtcgg gaagcgcacc cgggacaatg ttcagttgtt gtattccaga gggcaacgtg
     3421 gcgtttttcg ctgctcccgc ctgacgcgcc agagaaaggc atagcgggcg tttttctgtt
     3481 tgcagcgtcg ccgttagcgc atttccccag tgggatttgc gcacgcttac ggttggaata
     3541 tctaacgaag tcacctggca gatgcttgca ccatgtaaac gccaggccag ccgggtcgac
     3601 agttcatcgc caaacgttcc cggcggaaag agaacaacgt ccgcaggcgt tcgctgccac
     3661 tgttccacca acgcatccag cacctgttcc gccactacag gctgaggttc aatctgccag
     3721 tgcgccaggg tacaaccact gaaatcctgc gccgccagcc agcttgctat tgcggcattc
     3781 tcctgattta ttgtcacaat tgcgatattc atggctgcat cctttggcgt aaatagtcct
     3841 gccatagctt ttgagctttt tctgctaccg tttgcccatc aatcagcgtc gctccgcgtc
     3901 gttgctctgc tcttgccagt tgcagacact gcatcgctgg catttcggca gcaacagttt
     3961 tacggataat ttctgctttc cccgcagcca ttcgctggcg cattcccggt actggcaagg
     4021 ccacttcacc gcactgacgc acggcaatca ccgcaggcaa gcgaacccgg caacaacgca
     4081 acccatgttc agtacgctgt tcgagggtga taaacagcgc gtcgagagtg aaacgctcca
     4141 cctgggtgaa gcaggtccag cccagcattt ccgccagcaa aaatggcgtt tgcccattct
     4201 gcccttcgct gctttggctg ccggtaatta tcagatccag cggattctga tgctgccatt
     4261 cggcaatatg gcgagcgaca aattccggcg caaagcgcag atctgccgcc gtctccagca
     4321 atacagcttc ctcaaa
//
LOCUS       DAJTOR010000238         4309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTOR010000238 DAJTOR010000000
VERSION     DAJTOR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.240"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..965)
                     /locus_tag="OO249_003910"
     CDS             complement(<1..965)
                     /locus_tag="OO249_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR5638026.1"
                     /translation="MIRKSATGVIVALAVIWGGGIWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELA"
     gene            complement(1120..1746)
                     /gene="dsbA"
                     /locus_tag="OO249_003911"
     CDS             complement(1120..1746)
                     /gene="dsbA"
                     /locus_tag="OO249_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5638027.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(1763..2749)
                     /gene="srkA"
                     /locus_tag="OO249_003912"
     CDS             complement(1763..2749)
                     /gene="srkA"
                     /locus_tag="OO249_003912"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5638028.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(2826..3095)
                     /locus_tag="OO249_003913"
     CDS             complement(2826..3095)
                     /locus_tag="OO249_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5638029.1"
                     /translation="MKCKRLNEVIEFLQPAWKKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            3165..3749
                     /gene="mobA"
                     /locus_tag="OO249_003914"
     CDS             3165..3749
                     /gene="mobA"
                     /locus_tag="OO249_003914"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5638030.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            3731..4258
                     /gene="mobB"
                     /locus_tag="OO249_003915"
     CDS             3731..4258
                     /gene="mobB"
                     /locus_tag="OO249_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5638031.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALYARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGARHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         1167 a         1017 c          963 g         1162 t
ORIGIN      
        1 tttgctaact cagggtgtgc agcaagctgc ttctgcatcg caatgttata ctggatgata
       61 aattggcgca ctgctgaggg atcaatagat tcagcaatca atgaccactc accactaccg
      121 agttgctgat tcccttttgt taacttatca agttcgtagg tcagattgac attgatataa
      181 tctttgtctt taacgcgatc cagcctggtt tttgcaacga aagcatcgat ttgggcaacg
      241 tcttccccct gattgataat attaacctga ttaaatttcg actcgctttc accaaccgga
      301 aatttctttt cttctaatcg tgtcagagaa tcaatggtaa aactttttgc cgttactttg
      361 ttcattggtg ataattgcag aacaatatct tcgactttgc cttcaatatc caggttagac
      421 aatgaactac catcaccatt aaaagtaaac tggccctcac caaaagaaaa ctttccatac
      481 tctgcagggg caacatctaa tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc
      541 gcttccacgg gcgatttatt tttcgcggcg ataaatagcg gttgtgtcag ttcattattc
      601 actaagttca cttttgccgc tgccagtgct gggatgacat taccatgcat taacattgtg
      661 atgggcaacg gaccgtgctc aacatccaca tcaaatgcaa ctttctggcc tggcttgata
      721 ttgagatcgg gtgcaccgtt atcaaatgaa atttgcattt gaaaatggga attaaaaaag
      781 cctttgtcaa agtttttata acttaacgtc atatcgtagg catgttcacc tttcttttta
      841 gcatcgttaa aatctttaat aaatttttcg acaccaggct gaatttgcgt acctgtgtac
      901 catatgccac caccccagat tacggctaac gcaacaataa cacctgtagc tgacttacgt
      961 atcatataaa tattccctga taaaacagca aaactttgaa tatccactta tgctgaaata
     1021 atgtccagcg gcaggatgta ttatcagaat gtatttatat ttattaaata ataaaaaaag
     1081 cccgtgaata ttcacgggct ttatgtaatt tacattgaat tattttttct cggacagata
     1141 tttcactgta tcagcatact gctgaacaaa aacatccata ttgctggtat ccataccctg
     1201 cggattcagc tgatatttac cgttaacaaa catcgccgga acaccgcgca attgcacgtc
     1261 agctgcagct ttttcctgct gagctaccag agatttcacc acgaagctgt tccacgccgc
     1321 gtcgtactct tcacctttaa tacctgcgtt gataaataca tcgcggatat cagaagcaga
     1381 acgaatggtc tgggttttct gtacgccttc aaacagcgga acagtcactt tgtcttccac
     1441 gcccagcgcc atcgccacag cccatgcctg agtcagatct ttgcccaggt caccacccat
     1501 gaagttgacg tggtatttag tcatcttcac gccttccggc agttttttct tcacattatc
     1561 agaaatatgc agaacttctt caaactgata gcagtgcgga cagaagaaag agaaaaactc
     1621 cagcacttgc ggcgcgccag ctaccggttt ttccagggta gtgtactgtt taccatcttc
     1681 atactgcgcc gccgatgcgc taaacgctaa aactaaacca gccagcgcca gccaaatctt
     1741 tttcatgatc tactctctcc gattaataca taggtgttaa ttgtaaaggg ggttcttgta
     1801 gaacttttgc ctgttctata aaagtcgccg tttgtcgcag ccagtaatct tccccggtta
     1861 accacgggaa atttttcggg aacgcgggat cagcccaacg ccgcattagc caggcaagat
     1921 aataaaccaa acgcatggcg cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact
     1981 cgctaaattc ttcataagct tcaataatag tttccagttg catccgctgc tcggctttat
     2041 cgccattgag caacatccac aaatcctgaa tagctggacc attacgtgca tcatccagat
     2101 caacaaacat tggaccatcg cgccagagaa tattcccggc gtggcagtct ccatgtaagc
     2161 gcagaacggt ggaatcttcc cgccagtgcg ctgtaacggc ggcgatcagc tcatctgtcg
     2221 ctttcaggaa tgccgctttc aacccggaag gtatcagtgt agcgtcctca aacagcttgc
     2281 gtggctcaat gagatattca ttcagaccga tggtcgggcg atggataaaa agctgtttgc
     2341 gccccgtctg gtgcatacgc cctaaataac gcccaaccgc ttccatctga tcgatattat
     2401 cagcttcgaa ctggcgacca ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat
     2461 tcaataaagt ctgaccgtta aaggccacag gtgctgcgac cggaacttca tcatttacca
     2521 actgcaacgc aaattgatgt tcttcgagga tttgatcggc tgtccaacgt tcagggcgat
     2581 aaaatttgac gacaatacgt cgacgatctt cgtcctgaaa ttgatagaca cggttttcat
     2641 agctgttaag cggggtaaga ccggaatcca cccggatccc ttgctcaaat agagcatcca
     2701 tgatggtatc cgggtgtagt gtctggaaag taaaagcgct gttgttcatc ccatcatccg
     2761 gaaaacatta cgaatgatga aggatatcat ttcacgatga ttttggtggc gccgcttaca
     2821 agcttttact ctttaattac gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca
     2881 taatctttct gcaaacccgg gatcaccgca tctttcgcgg aatcacgcat tttcaggtga
     2941 tagatcagaa tgtcatccgt caaatccgcc agttcgccgt caaaacctga ctctttcgcc
     3001 agtttctgca aaaattgcaa caggttaagg tctggctctt ttttccaggc tggctggagg
     3061 aattcaataa cttcattcag acgtttacat ttcatagtga tgctccttac tcttgagaca
     3121 gacacgttag cagggtcaat cccacaataa aagaggcgat atcggtgaat ctgatgacga
     3181 cgataacagg cgttgtgctg gcaggcggta aagccagacg aatgggtggc gtagataaag
     3241 gattgcttga attaaacggc aaaccattat ggcaacatgt cgctgacgcg cttatgacgc
     3301 agctctctca cgtcgtggtt aatgccaatc gtcatcagga aatctatcag gcaagcggtc
     3361 tgaaagtgat tgaagattca ctggcggatt acccaggccc tctggcagga atgctttcag
     3421 taatgcagca ggaagctggt gagtggtttt tgttttgccc gtgcgatacg ccttacattc
     3481 cccctgattt agcagcccgg cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc
     3541 atgacggtga acgcgatcac ccgactattg ctctggtaaa ccgcgctatt gagcctttat
     3601 tactggaata tctgcaagca ggagaacgcc gggtaatggc atttatgcgt ctggctggcg
     3661 gtcatgcggt tgatttcagc gatcataaag atgcatttgt taacgtgaat acgccagagg
     3721 agcttgcccg atggcaggaa aaacgatgat accgttactc gcctttgccg cgtggagtgg
     3781 taccggaaaa actacgcttt tgaaaaaact gatcccggca ttatacgcca gagggatccg
     3841 tccagggctg attaagcata cgcaccatga tatggatgtt gataagccag gcaaagatag
     3901 ctatgagctg cgcaaggctg gcgcggcaca aaccatcgtt gccagccagc aacgatgggc
     3961 cttgatgacg gaaacaccag acgaagaaga gctggatcta cagtttctcg caagtcgaat
     4021 ggatacctca aagctggatt tgattctggt cgaagggttt aagcatgaag agatcgcaaa
     4081 gattgtgctg tttcgcgatg gagccagaca tcgacctgaa gaattagtga tagacaggca
     4141 tgttattgct gtagccagtg atgtgccgct taatcttgat gtcgcattac tggatattaa
     4201 tgatgttgag gggctggctg attttgtggt ggagtggatg caaaagcaga acggataagc
     4261 aagagtaggc tggatcagac gcagccagtg tcgcatccga cctttttct
//
LOCUS       DAJTOR010000239         4302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTOR010000239 DAJTOR010000000
VERSION     DAJTOR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.241"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            55..2217
                     /gene="ybiO"
                     /locus_tag="OO249_003916"
     CDS             55..2217
                     /gene="ybiO"
                     /locus_tag="OO249_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR5638032.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGLWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLVGVRAPVQTY"
     gene            complement(2277..3203)
                     /gene="rlmF"
                     /locus_tag="OO249_003917"
     CDS             complement(2277..3203)
                     /gene="rlmF"
                     /locus_tag="OO249_003917"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5638033.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3479..3738
                     /locus_tag="OO249_003918"
                     /pseudo
     CDS             3479..3738
                     /locus_tag="OO249_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            4003..>4302
                     /locus_tag="OO249_003919"
     CDS             4003..>4302
                     /locus_tag="OO249_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5638034.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT          998 a         1139 c         1076 g         1089 t
ORIGIN      
        1 ccccccccag catctatact tatctttttg ctctgttttc tcactggagg agtcatgcgg
       61 tggatcctgt tcatcctctt ctgcctgctg ggcgcacctg cccacgcggt atccataccc
      121 ggcgttacaa ccacaacgac aacggactca acgactgaac cggccccgga accggatatc
      181 gaacaaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
      241 aaagaattga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
      301 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
      361 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatattacc
      421 ggctccccgc ataagccgtt taatccacaa acattcagca atgcgctgac ccatttttca
      481 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgctg
      541 tatcgcaaaa tgggcctgtg ggcgcggcaa aaaaatcgtg agcgcagtaa ctggttgcag
      601 cttccggcga tgattatcgg ggcgtttatt atcgacctac tgttactggc actgacatta
      661 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
      721 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagctgt actacgcctg
      781 attttttgcc caaacgtggc tgaactgcgc ccgttcacga ttcatgacga gaccgcccgt
      841 tactggagtc gtcgcctgag ctggttaagc agtttgatag gttatggcct gattgtggcc
      901 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
      961 atgctgtgca tgaccgtctg ggcgttgtac ctgatctttc gcaataaaaa agagattacc
     1021 caacatttgc tcaactttgc ggagcattcg ctggcctttt tcagcctgtt tatccgcgcc
     1081 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcgtgctgtt tttcttttcg
     1141 ttgttcgatc cgggcaacag cctgaaattt atgatgggtg caacggtgcg cagcctggcg
     1201 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc
     1261 accctctcgc cacatactca gcgtaactat ccggagttgc aaaaacggct gaatggctgg
     1321 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcggt aatgttgctg
     1381 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
     1441 accgtagata tcctgatccg tatcgcactt attctttttt tctcggcggt tggctggacg
     1501 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
     1561 agcgcccgta cgcgtaccct gctgacgctg tttcgtaacg cgctggcggt gattatcagt
     1621 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
     1681 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt gaaagatatc
     1741 attaccgggg tgtttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
     1801 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
     1861 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
     1921 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
     1981 aatcaggcac tgaaagatgc ggtagcggaa ttgatggaaa acgaagaaat tcgcgggctg
     2041 attattggtg aaccgaattt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
     2101 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
     2161 caggtgaaaa aacatttcga cctggtgggc gttcgcgcgc cagtgcagac ttattaggtg
     2221 ctgcctactc cgggcgcgac cccggctgaa ccgttaccgc cgggggagcc aacgctttaa
     2281 cgctggcgat tgacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
     2341 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
     2401 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
     2461 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa ggcgacttca
     2521 ccaccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
     2581 agccccaggt tacggcgttt acgctcacta cctgcccgcg ctgcagcggc ggaatcgtgg
     2641 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tatggatgat gccgttaaaa
     2701 attgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
     2761 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
     2821 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
     2881 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
     2941 tgaatataat ccgcccggcc gggtactggt gggcagagaa aaccgtcggg gatatcccag
     3001 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
     3061 ttggcaaagt ctacgctttg ctccccggcg ggtgtaagcg tgaggaattg cctgagttca
     3121 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
     3181 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
     3241 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
     3301 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt
     3361 tgtaaaaata tatcgttcaa aaaataagca catctctgtc aattttttgc gtcatttgca
     3421 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
     3481 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc agaatctctc tcacggctta
     3541 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgcgg taccgtttcg
     3601 gcaactggcg catcaaacct aagcgatctg gaggataaac tggcagaaaa agcgcgcgaa
     3661 caaggcgcga aaggttatgt cattaattcc gcaggcggaa atgaccagat gttcggtact
     3721 gcaaccatct acaaataacc ccaccaacgt aactgcattc gcctctggat gcagttttcg
     3781 cctgttttgt aatcaaaaaa catcaaatat gaactcaatg taaataaatg tatttctttt
     3841 tcgcgcaatg ggtgatagaa aatcgcgcca agtgataatg cttatcaaaa ttattatcac
     3901 ttgcacgaac actatcacgg gattaacagt ggcatcgcat ccgcagagat gctttctcgt
     3961 gacagtgaaa atttcaacat ataagaaaaa gtcacctgca aaatggaaaa caatcgcaat
     4021 ttccctgcca gacaatttca ttcgctcacg ttctttgccg gtctttgtat tggcatcacg
     4081 cctgtggctc aggcactcgc cgccgaaggg caaactaacg cggatgacac gctggttgtc
     4141 gaagcatcaa cgccttcgct ttatgcgcca caacaatctg ccgatccgaa attctcgcgt
     4201 ccggtagcgg atactacccg cacgatgacg gtgatttctg aacaagtgat taaagatcag
     4261 ggcgcaacca accttaccga cgcgctcaaa aacgtccccg gc
//
LOCUS       DAJTOR010000240         4238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTOR010000240 DAJTOR010000000
VERSION     DAJTOR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.244"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..200)
                     /locus_tag="OO249_003920"
     CDS             complement(<1..200)
                     /locus_tag="OO249_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5638035.1"
                     /translation="MGNYIRPLSDAVFTIASDDQWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRF"
     gene            complement(446..898)
                     /locus_tag="OO249_003921"
     CDS             complement(446..898)
                     /locus_tag="OO249_003921"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5638036.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(895..1947)
                     /gene="dinB"
                     /locus_tag="OO249_003922"
     CDS             complement(895..1947)
                     /gene="dinB"
                     /locus_tag="OO249_003922"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5638037.1"
                     /translation="MKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTAN
                     YPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLD
                     EAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQ
                     FVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFG
                     RILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKV
                     KPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGLH
                     VTLLDPQMERQLVLGL"
     gene            complement(2006..2776)
                     /locus_tag="OO249_003923"
     CDS             complement(2006..2776)
                     /locus_tag="OO249_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638038.1"
                     /translation="MIYSNQLRYTGKDGVYFHLRDNVDAFLTLSATENMEFDSPMQVT
                     TQNMQSGQTVTDNVQRAPRTITISGVVVVGESGSILISRDTKLVENFIDTLENWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATG
                     KTATTNDGGATSKKNTGNTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSV
                     KNGESTFTASANEQASKVLKSGNALRKYSVNPNKKGTY"
     gene            complement(2934..>4238)
                     /locus_tag="OO249_003924"
     CDS             complement(2934..>4238)
                     /locus_tag="OO249_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR5638039.1"
                     /translation="TTGLDIAELQSQFAGFGASAKESMGIQGSEELFRNMIGYSRLMG
                     RSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKSEQELFDAMK
                     KGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSEN
                     GLQDFTRSLTTLLNSLGGSGKSLGESLGSLMTSMSSGVDSLTDISYRVRAFFDEVTLA
                     YRGLTDEQKKVVDALGSGLINSLKVLAGVIAVNKVVGVTRSIYGLMTAITRLGQVADT
                     TADNVNRKNGKPSRLNPMKMLLAGSAIYAGYERLASTPERMEAQAKLPELAKESGLKP
                     AFTMDDVKGWFNSAAMNLANFQHPELNNIKQDSALTSADIQSLRDEISALSKRIQEPV
                     KVSLGGEVAIKPDETSFMTFSSNIYDQYAEATLLSSSFPEDD"
BASE COUNT         1135 a         1005 c          874 g         1224 t
ORIGIN      
        1 taaaaacgac caacagagaa gaacgctgcg ccaatcggat agccgcgtcc gggatgtaaa
       61 tctggcatcc ccactacgcg ctgcatgttg ggtaaatttg ccgtggtgtg taattgttgg
      121 atcgctaaac tctcgatcca ctggtcatca gatgcgatgg taaataccgc atcggataag
      181 ggacgaatat aattgcccat ataccattcc gtaaatagac tcgtcatact tcaagttgca
      241 tgtgttgcga cttgaattat tgagagtaac aattaaaaaa tcaggaaatg gcagacgaat
      301 ccagggcaag aaaaaaataa ttaatcctgg aaaggtctgc aaagagtcat tgataaagga
      361 tttgtcatga tttacctcct tgcagtttgt ggaataagaa aacgcgcaca tactaggctt
      421 gcgttatttt cattgcaagc tggatttaat gttgctgttt ataacgcata taaaaattaa
      481 taaaccactc tccccgacat tcaacgcgtt gctgcttaac tgtctgaaaa ccataacgtt
      541 caaaaaaggg ttttgcggtt atgcttgcgt ccaccgtaag ttcggattca gacttaatca
      601 aaggttttaa caaagcgctg gcaaccccac ggcgggtgta ttcagggtca acaaataaca
      661 tatcgatata gtgttcaacg caggtaataa agccgaccag ttttgcatta atgactgcaa
      721 cccgcacttg tgatttcgcg agtttctcct tccagcgaga ttcgtcaatc tgcgcccagg
      781 cggcaatttg ttgtggtgaa taatgctgac tggcagtcat cgtaaccgct ctaatgaaaa
      841 tagcgcatag ttgctgaaaa tcgccaggct gatagtctct tatttgtatg ttattcataa
      901 tcccagcacg agttgtcttt ccatttgcgg gtcaagcaac gtcacatgca gccccaccag
      961 tcgcacaccg cgcccgccgc ggcgttcatc ccaggtttta cgcgcggtgg cgattaagtc
     1021 agctttattc agccgcggcc agacgtgctc ctgagtggtt tgctgaaaat catcaaactt
     1081 taatttcacc ccctggcgag caatcagtaa atcaggtttc acctttgcca gacggcgttc
     1141 aagttccgga tacagccgct cgataatcgc ttcacattca gaccagtggt ggatatcttc
     1201 cgccatcgtg cgttccacgc cgacggattt tcgcaaccgt tcgctgttaa cgtcgcgctc
     1261 gtcaatcccc tgactacgct cccacaaaat gcggccaaat ttgccaaagc gtttaagcag
     1321 catcaccaga tcacactttt gtacatcacc gcaggttcgt agccccatcg cttccagttt
     1381 tgccgccgag actttgccga cgccggggat ttttgccagt ggtaaggttt gtaaaaatgc
     1441 cggaacttct gccggcgtaa tcacaaactg gccgttgggt ttattcatgt cggaggcgat
     1501 tttggcgaga aactttacgg gtgccacgcc cgcagacgcc gtcagttgca gctcgttgaa
     1561 aatcgtctgg cggatttcct gggcgatgag ggtcgcagaa ccgtggcaat ggacgctatc
     1621 ggtgacgtcg agataagcct catccagtga caacggttca atacgcgagg tgtagcgcga
     1681 gaagatttcg cggatatgat ttgaggcttc tttgtaggcg tcaaagcgcc ccggaagcaa
     1741 ggtgagatgc gggcataatt tgagcgccat ccctgtcggc atagcgctac gtacgccaaa
     1801 tttacgcgcg ggataattgg cggtactgat caccccccga cgttcgcggc tgccgccaat
     1861 agcaataggg atatcgcgca gggcgggatt gtcgcgcatc tccaccgccg cgaaaaagca
     1921 gtccatatcc acatgaataa tcttcacttt cttatacacc ctgtatattt atacatgcaa
     1981 agagattata cacacaaaag caaaatcaat aagtgccctt cttattcgga ttcacagagt
     2041 acttccgtaa agcattaccc gacttaagca cctttgaagc ttgctcatta gctgaggctg
     2101 tgaaggtgct ctcaccattt ttaacgctaa cgctcttaga gcacgttaca cgggctttta
     2161 gcgcttcatc tgataactca cttgccgaat agctaaaaag ctctttacag gtcagcatag
     2221 ggcttccaag gctcgttgta gtgttgccag tattcttttt actggtagca ccaccatcat
     2281 tcgtggtagc agtcttgcca gttgcagccg aaatatcagt ctgacccaca atagctttga
     2341 agttaatttc ctgaaaagtt agctggattc ttagaccatt tgaaatacca acatctttag
     2401 aggctttaaa gctcgtgata atggaatcat caattttaat tccgtcttta cagatgaccg
     2461 aaataatctg cttctggtca cgccagtttt ccagagtgtc gatgaagttc tccaccagct
     2521 tagtatctcg tgagataagg atactaccgg attcaccaac tacaacgaca ccactaatag
     2581 tgattgttct gggtgctctt tgcacattat ctgtgacggt ttgccctgat tgcatgttct
     2641 gtgtagtcac ctgcataggg ctgtcaaatt ccatgttttc agttgctgat aaggttaaga
     2701 aggcatctac attatctctt aagtggaagt aaacgccatc tttgccagtg taacgaagtt
     2761 gattagagta aatcattaaa ttctcctttg atttcaaagg cttagtgtat tttaatcatt
     2821 tttaaccaaa cctttgatat ataagtattt tctctgttaa gcgtgagcta cgtgaaaaat
     2881 aatttttggt gtgggtatta actttgcttt attcacgtag cattcgacta ttattaatca
     2941 tcttctggga atgatgaact taatagcgta gcttctgcat attggtcgta gatattactg
     3001 ctaaaggtca taaagctggt ttcatctggt ttaatggcaa cttcaccacc aagtgaaact
     3061 ttcacaggct cctgaatacg tttgctcaaa gcggagattt catctctcag gctttggata
     3121 tctgcactcg ttaatgctga atcttgctta atgttgttta attctggatg ctggaagtta
     3181 gcaagattca tagcagcact attaaaccac cctttaacat catccatagt gaaagcgggt
     3241 ttaagtccag actctttagc cagctctggt aatttagctt gtgcttccat tcgttcaggt
     3301 gtagaagcta atctttcata acctgcatag atagcactac cagctaatag catcttcata
     3361 gggtttagtc ttgatggttt cccattcttt ctattcacgt tatccgctgt agtatcagct
     3421 acctgaccta atcttgtgat agcagtcata agcccgtaaa tacttctggt tacaccaaca
     3481 actttattca cagcaataac accagccaag acttttaaac tattaatcaa gccagaacct
     3541 aaagcatcaa caactttctt ttgttcatcg gttaaaccac gataagctaa tgtcacctca
     3601 tcaaagaatg ctctcactct gtaactgata tcggtcaggc tatccacacc acttgacata
     3661 gaagtcatca agctaccaag agattcacca agactcttac cacttccacc taaactgttt
     3721 aaaagcgttg ttaggcttcg tgtaaaatct tgcagaccat tttcagaacc tctgaagata
     3781 ctatcaagcg tggtattcca agagtttttc aggttcccca gttgtgcttg tgtgctttct
     3841 gaaatagctt tccagccacc tttagctgtg atttgtttat tcagttcctg agtgaccttc
     3901 tgaagagtat cggcagattt taaagccccc ttcttcatgg catcaaataa ttcctgttca
     3961 ctctttccgg ttgctttagc gaatacctga accataccag gaacagcttc agctagttgc
     4021 cccttaagct cttcagccat aatctgaccc ttaccagcca tctgtgataa tgctgtcagt
     4081 gctcgcttaa tctcttcctc acttcgaccc attaaacgag aatagccaat catatttcta
     4141 aaaagctctt cagaaccttg aatacccata gactctttag ctgaagcacc aaaaccagca
     4201 aactgtgatt gcagttcagc gatatctaaa cccgttgt
//
LOCUS       DAJTOR010000241         4205 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTOR010000241 DAJTOR010000000
VERSION     DAJTOR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.245"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(24..1238)
                     /gene="ydcO"
                     /locus_tag="OO249_003925"
     CDS             complement(24..1238)
                     /gene="ydcO"
                     /locus_tag="OO249_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCR5638040.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVARATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAEILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTVASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITEMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNQY"
     gene            1291..1827
                     /gene="sutR"
                     /locus_tag="OO249_003926"
     CDS             1291..1827
                     /gene="sutR"
                     /locus_tag="OO249_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCR5638041.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1900..3861
                     /gene="rlhA"
                     /locus_tag="OO249_003927"
     CDS             1900..3861
                     /gene="rlhA"
                     /locus_tag="OO249_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR5638042.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMNYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVAEPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3953..4183)
                     /locus_tag="OO249_003928"
     CDS             complement(3953..4183)
                     /locus_tag="OO249_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCR5638043.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1094 a         1095 c         1054 g          962 t
ORIGIN      
        1 cgtctttcag ggcgtgtagg atgtcaatat tggtttctgt cagcgattaa attcaacacc
       61 acgtaacaaa cgcctccggc aattaatccc cagaacgcag aaccaatccc gaccagcgtc
      121 aatccacttg ccgttaccag aaatgccacc accgccgcgt cgcgctcacg ctcattatgc
      181 agcgcctgat acaaactgcc gccaatggta ctcaacagcg ccagacctgc cagcatctgg
      241 atccaactta cgggcagggc agccatcatc tcggtaatgg cactaccaaa cagacctgcg
      301 agcaaataga aaatgcctgc aacggcagct gccagccaac gttgatcttt atccggatgc
      361 gcttccgggc tttggcaaat agccgcggtg attgccgcaa taccgacgga ataaacgccg
      421 aaaggggaaa aaaccagtgc cagcaatcca gtaaatacaa ttaatggcga aacaggagcc
      481 gaatatccag ctgctttcat tgctgcgata cccggtgcgt tttgcgatgc caccgtcacc
      541 agaaaaaggg ggagtgcaac gctcaggctg tgagcaaacg aaaaatcagg ggtaatataa
      601 gtggggagaa cgggtttaaa gacaacatca gttgtgacaa cgtcaccttg cgcgatgacg
      661 atcacgatcc caataatcat cgcggcaatt accgcatagc gcggcgcaac ggccttggtt
      721 gccagccata ccagcaacat acttccacac aacgtaaatt gaccgtccag actggcaaac
      781 gcctgtaaac caaagcgtaa taaaatctcg gcaagcattg ccgccgcaag cgagtgcgga
      841 ataatgcgca tcagacgagc aaagagtccc gttatgccgc agaggactat taacgcgttg
      901 gtgacaataa aaacgccgat ggcttcgtta agtgttagtc cctgcaatcc ggtgaccaac
      961 aaagccgcgc caggcgttga ccatgcggtg agaacaggta cgcgatacca taatgtcaga
     1021 gtcagcgtac tgacgcccat tgccagcccc agcgccgtca tccagccaga gatttgtgca
     1081 gtggtggctc tggcgacaat cgctgcttgc cagattattg ccgctgaact ggcgtagcca
     1141 attaataccg ccagaaaccc cgccagtagc gtgggtggag gaatagaaaa cagacgcata
     1201 gtcacctcgt acgttatagc gtacaggcac cgtagcattt gtccgttata acgcacaagt
     1261 gataaacttc cgttttgccg gaggagtcgc atggaaaatc tcgctcgctt tttatccacc
     1321 acacttaaac aactacgcca gcagcgcggc tggagtcttt cgcgactggc agaagcgacg
     1381 ggcgtttcta aagcaatgtt agggcagatt gagcgtaatg agtccagtcc gacggtcgcg
     1441 acattatgga aaattgccac cgggttgaac gtaccatttt caacatttat ttctccgccg
     1501 cagtccgcta cgccttcagt ttatgatcca caacagcagg cgatggtgat cacttcgctg
     1561 tttccttatg atccacaact ctgctttgaa cacttctcga ttcagatggc atctggtgca
     1621 ataagcgaat cgacgcccca tgagaagggg gtaatcgaac atgtcgtcgt cattgatgga
     1681 caacttgatc tgtgcgttga tggggaatgg cagaccctta attgtgggga aggcgttcga
     1741 tttgctgcag acgtcacgca tatctatcgt aatggtgggg agcaaaccgt acattttcat
     1801 tccctcatcc attacccgcg cagttaagcg ggaaaactat ttcgcaacgt cgcgcttgtg
     1861 gctaaaatag ccgccatttt tcagctactg gataagaatg tgaccgtatc ttctcatcga
     1921 cttgaactgt taagcccggc acgcgatgcc gccattgccc gcgaagctat tttgcacggt
     1981 gccgatgctg tttatatcgg cggccctggt tttggtgccc gtcataatgc cagtaatagc
     2041 ctgaaagata ttgccgagct ggtgccgttt gcccatcgtt atggtgcaaa aattttcgtc
     2101 acgcttaaca ccattttgca tgatgatgag ctggaacccg cgcaacggct gattactgac
     2161 ctctaccaga ccggtgtcga tgcgctgatt gttcaggata tggggattct ggaacttgat
     2221 attccgccga ttgaactgca cgccagtacg cagtgcgaca ttcgtacagt tgaaaaagcg
     2281 aagttcctct ctgatgttgg cttcacgcag attgtgctgg cgcgagagct gaatcttgat
     2341 cagatccgcg cgattcacca ggctacggac gcgaccattg aattctttat tcatggggca
     2401 ctgtgcgtgg cctattcggg tcagtgctac atttctcatg cgcaaacagg gcgtagcgcc
     2461 aaccgtggcg attgctcgca ggcgtgccgt ttgccataca cattgaaaga cgatcagggg
     2521 cgggtggttt cctatgaaaa acatctgctg tcgatgaaag ataacgatca gactgccaac
     2581 ctcggcgcgc tgattgatgc tggtgtacgc tccttcaaga ttgaagggcg ttacaaagat
     2641 atgaactacg tgaagaatat caccgcccat tatcgccaga tgcttgatgc cattattgaa
     2701 gaacgtggcg atctggcgcg cgcttcatca ggtcgtactg aacatttctt tgttccatcg
     2761 acggaaaaga ctttccaccg tggtagcaca gattattttg tgaatgcccg taaaggcgat
     2821 attggcgcgt tcgattcgcc gaaatttatc ggcctgccgg tgggtgaagt attgaaagtg
     2881 gcgaaagatc atctcgatgt tgccgtcacc gagccactgg caaatggtga tggcctgaac
     2941 gtgatgatta aacgtgaagt catcggtttt cgtgccaata cggtcgagaa aaccggagaa
     3001 aatcagtacc gcgtctggcc caatgaaatg ccagcagatt tgcacaaaat tcgtccacat
     3061 cacccactaa accgtaatct tgatcataac tggcagcagg cactgacaaa aacctccagc
     3121 gaacgtcggg tggcggtaga cattgaactg ggcggctggc aggaacaact gattctgacc
     3181 ctcaccagtg aagagggtgt cagcatcacg catacgctgg acgggcagtt cgacgaagcc
     3241 aataacgccg aaaaagcaat gaacaatctg aaggatggtc tggcaaaact ggggcaaacc
     3301 ctctattacg cccgcgatgt gcaaattaat ttgccggggg cgctgtttgt accaaacagt
     3361 ctgttaaacc agttccgccg tgaagctgct gacatgctgg atgctgcgcg tcttgccagt
     3421 taccagcgcg gcagccgtaa accggttgct gagcctgcgc cggtttatcc gcaaacgcat
     3481 ctgagtttcc tcgcgaacgt atacaaccag aaagcgcgtg aattttatca tcgctatggt
     3541 gtgcagctga ttgacgcggc gtatgaggca catgaagaga agggcgaagt cccggtgatg
     3601 atcaccaagc attgtctgcg ctttgccttt aatctgtgcc cgaaacaggc gaaaggcaat
     3661 atcaaaagct ggaaggcgac gccaatgcaa ctggttaacg gcgatgaagt attaacgcta
     3721 aagtttgatt gccgcccatg cgagatgcac gtcattggca aaatcaaaaa tcacatactg
     3781 aaaatgccgt taccgggaag cgtagtggca tccgtaagtc cggatgagct gctgaaaaca
     3841 ttgcctaagc gaaaagggta aaacgccagt tttctggtta ctcacaactc attgaatctg
     3901 catgatattg cctgccgggt aaggcgtcac gccgcatccg gcatcaaacg actcaacgtt
     3961 gtcccgcttc tggtttgcgc gatttttgcc agtaaaaatg ctcgcgcaaa ccttccgccg
     4021 actcttccgc cacagcacgc aattcatcgc tgtccgcttc atgacgcagc tgatgatcca
     4081 cattctttac ccacacaaat tcatgtcctt tgtgccctgc catgagttgt cctgagaaca
     4141 gcgcacacgt taataagaca accgataacg ccttcgtaaa catcctgcca cctttttctt
     4201 acctt
//
LOCUS       DAJTOR010000242         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTOR010000242 DAJTOR010000000
VERSION     DAJTOR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.246"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO249_003929"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO249_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5638044.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO249_003930"
     tRNA            complement(324..399)
                     /locus_tag="OO249_003930"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO249_003931"
     tRNA            complement(406..480)
                     /locus_tag="OO249_003931"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO249_003932"
     tRNA            complement(597..681)
                     /locus_tag="OO249_003932"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO249_003933"
     tRNA            complement(690..765)
                     /locus_tag="OO249_003933"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO249_003934"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO249_003934"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5638045.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO249_003935"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO249_003935"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5638046.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO249_003936"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO249_003936"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5638047.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNVVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1079 a         1041 c          932 g         1131 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctaggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc acattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tag
//
LOCUS       DAJTOR010000243         4176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTOR010000243 DAJTOR010000000
VERSION     DAJTOR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.247"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(43..213)
                     /locus_tag="OO249_003937"
                     /pseudo
     CDS             complement(43..213)
                     /locus_tag="OO249_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO249_003938"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO249_003938"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5638048.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO249_003939"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO249_003939"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5638049.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDSQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO249_003940"
     CDS             2894..3634
                     /locus_tag="OO249_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5638050.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3669..>4176
                     /gene="ruvC"
                     /locus_tag="OO249_003941"
     CDS             3669..>4176
                     /gene="ruvC"
                     /locus_tag="OO249_003941"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5638051.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLAR"
BASE COUNT         1033 a         1030 c         1176 g          937 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagat cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggctaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggtcacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaatcga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgtccgga aatggctcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgagttta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgcgatg
     1741 ggcgcactgc gcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttattgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gcagagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 attgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgacagtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcatgc
     3241 attcagcaaa tgtggcggta acctcggtac agatggttcc gtagcctatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga tgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacctatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg caccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgagattg cagcgactct ctgatgaggc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctatggcg
     3721 tcatccgcca ggtaggcagg caactgtcct acctgggtag cggatgcata cgcaccaagg
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc agattatttc gccattgaac aagtctttat ggccaaaaat gccgactcgg
     3901 ctctgaaact ggggcaggcg cgcggcgtgg cgattgtggc ggcggtgaat caggaattgc
     3961 cggtatttga atatgcggca cgtcaggtaa agcaaacggt ggtaggtatt ggtagtgccg
     4021 aaaaaagtca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgtta gctatcgcga ttacccattg tcacgtcagc cagaatgcaa
     4141 tgcagatgag cgaatcgcgg ctgaacctgg cgagag
//
LOCUS       DAJTOR010000244         4099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTOR010000244 DAJTOR010000000
VERSION     DAJTOR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.248"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..178
                     /locus_tag="OO249_003942"
     CDS             <1..178
                     /locus_tag="OO249_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5638052.1"
                     /translation="HDVLTGSDDGRHSNTSLAWGAGVQFNPTESVAIDVAYEGSGSGD
                     WRTDGFIVGVGYKF"
     gene            243..1647
                     /locus_tag="OO249_003943"
                     /pseudo
     CDS             243..1647
                     /locus_tag="OO249_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            1596..1748
                     /locus_tag="OO249_003944"
                     /pseudo
     CDS             1596..1748
                     /locus_tag="OO249_003944"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1725..1904
                     /locus_tag="OO249_003945"
                     /pseudo
     CDS             1725..1904
                     /locus_tag="OO249_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            2619..3266
                     /locus_tag="OO249_003946"
                     /pseudo
     CDS             2619..3266
                     /locus_tag="OO249_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            3428..3850
                     /locus_tag="OO249_003947"
     CDS             3428..3850
                     /locus_tag="OO249_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5638053.1"
                     /translation="MAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVT
                     WLSPEGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIA
                     TEEETSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(3905..>4099)
                     /locus_tag="OO249_003948"
     CDS             complement(3905..>4099)
                     /locus_tag="OO249_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCR5638054.1"
                     /translation="TILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHF
                     ENVKTKVQGTVVMFSASGKK"
BASE COUNT         1089 a         1047 c         1137 g          826 t
ORIGIN      
        1 gcacgatgtg ctgaccggaa gtgatgacgg tcgccacagc aacacgtctc tggcgtgggg
       61 agctggcgtg cagtttaacc cgaccgaatc cgtggccatt gatgtcgctt atgaaggctc
      121 cggcagtggt gactggcgca ctgacgggtt catcgtgggt gttggttata aattctgatt
      181 agccaggtaa cacagtgtta tgacagcccg ccggttcagg cgggcttttt tgtggggtga
      241 atatggcagt aaaaatttca ggtgtactga aagacggcac aggaaaaccg gtacagaact
      301 gcacaatcca gctgaaagca aaacgtaaca gtaccacggt ggtggtgaac acgctggcat
      361 ctgaaaatcc ggatgaagcc gggcgttaca gcatggacgt tgagtacggt cagtacagcg
      421 ttattctgtt ggtggaagga ttcccgccgt cacatgccgg gaccatcacc gtgtatgaag
      481 attcccgacc cggtacgctg aatgattttc tcggtgccat gacggaggat gatgcccgtc
      541 cggaggcact gcgccgtttt gagctgatgg tggaagaggt ggcgcgtaac gcgtccgtgg
      601 tggcacagaa cacggcagcc gcgaagaagt cagccagtga tgccagcaca tcagcccgtg
      661 aggcggcaac ccatgcgact gatgctgcag gctcagcacg cgcagccagc acgtcagccg
      721 gacaggccgc gtcgtcggct cagtcagcga cttccagcgc aggaacggca tcaacaaagg
      781 ccactgaagc atcaaaaagt gctgccgctg cagagtcctc aaaaagcgcg gcagctacca
      841 gtgccagtgc cgcgaaaacg tcagaaacga atgctgcagc gtcacaacaa tcagcagcca
      901 cttctgcatc caccgcgact acgaaagcgt cagaagctgc cacctcagcc cgggatgcgg
      961 cggcttcaaa agaggcggca aaatcatcag aaacgaacgc atcctcgagc gccagtagcg
     1021 cagcttcctc ggcaacagcg gcaggaaatt ccgcgaaggc ggcaaaaacg tcagagacga
     1081 acgccaggtc ttctgaaacg gcagcgggac agagcgcctc ggctgcggca ggctcaaaaa
     1141 cagcggctgc gtcgtctgcc agtgcagcgt caacaagtgc cgggcaggcc tcagccagtg
     1201 ccaccgccgc cggaaaatcg gcagaaagcg ccgcatcgtc tgcttcaaca gccacaacga
     1261 aggctggcga agccactgaa caggccagcg cagcagcgag gtctgcttcc gcagcgaaga
     1321 catccgaaac gaacgcgaaa gcgtcggaaa ccagcgcaga atcctcaaaa acggctgccg
     1381 catcgtcagc cagttcggcg tcgtcatcgg cctcatcggc gtctgcttca aaagatgagg
     1441 cgaccagaca ggcgtcagca gcgaagggta gcgccacgac ggcatccacg aaggcgacag
     1501 aggcagctgg cagtgcgacg gcggcagctc agagcaaaag tacggcggaa tctgcagcaa
     1561 cgcgcgccga gacagcggca aaacgggcag aggatattgc atccgccgtg gcgcttgagg
     1621 atgcgagcac gacgaaaaag gggatagtac agctcagcag tgcgaccaac agcacttccg
     1681 agtcactggc ggcaacgcca aaagccgtta aggccgcgta tgacctggct aacgggaaat
     1741 acaccgcaca ggatgcaacg acagcacaga aagggataat ccagctaagc agcgcgacca
     1801 acagcacgtc tgaaacgctg gcggcaacgc caaaggcagt aaaagcagcc aatgacaatg
     1861 ctgagaaacg tctgcagaaa gatcagaacg gtgcggatat ccctggcaaa gacaccttta
     1921 cgaaaaatat tggtgcctgc cgtgccttcg gtgggtcagt aagcacaaca acaggaaact
     1981 ggacgactgc acagtttatc gagtggctgg attctcaggg agcatttaac catccatact
     2041 ggatgtgcaa gggttcctgg tcttatggca ataataaaat cattactgat actggctgcg
     2101 gtaatattca tctcgccgga gctgtcattg aagtaatggg gataaagtca gcgatgacga
     2161 tccgcattac cacaccgacc acctccactg gtggtggaac aactaacgcc cagtttacct
     2221 atattaatca cggaacagat tattcacctg gctggcgaag ggactataac tccagaaata
     2281 agccaacggc atcagagatc ggggcgttac cgtcaggtgg aacagcagta tcatcagttg
     2341 atctgtcttc aaaaggtcgg gtaaccgcgc tgacagacaa tacgcagggg gcaacaggtc
     2401 ttgagttata cgaggtgtat aacaacggat atccaacagc gtatggaaat atcattcacc
     2461 tgaaagggat gacagccgtt ggcgaaggtg agttactcat cggctggagt ggtacaagcg
     2521 gtgctcatgc tccggcattt attcgttcac gacgggatac gaccgacgca aactggtcgc
     2581 cgtgggcgca gctttacacc tcggctcatc ctcctgcaga gttttatcca gtcggtgcac
     2641 caatcccgtg gccatcagat accgttccgt ctggttatgc cctgatgcag gggcagactt
     2701 ttgacaaatc tgcttacccg aaacttgcag ccgcttatcc gtcaggcgtg atccctgata
     2761 tgcgtggctg gacgattaag ggcaagcccg ccagtgatcg agccgtattg tctcaggaac
     2821 aggacggcat taaatcacac acccacagcg ccagcgtatc cagtacggat ttggggacga
     2881 aaaccacatc gtcgtttgat tacggcacta aatccacgaa taacactggt gcgcataccc
     2941 atagtgttag cggtacggct gcttcagccg gtgcacatac ccattcgatg acatttgttt
     3001 caggtggttc cagtggtgct ccgggaagtg gatcacctga ttattctaaa tacagtgtta
     3061 acacttcttc tgcaggcgct catacgcact ctgtatcggg tactgctgca agcgcaggtg
     3121 cacacgcaca tactgtcggt attggtgctc atacgcactc cgttgcgatt ggttcacatg
     3181 gacataccat caccgttaac gctgctggta acgcggaaaa caccgtcaaa aacatcgcat
     3241 ttaactatat tgtgaggctt gcataatggc attcagaatg agtgaacaac cacggaccat
     3301 aaaaatttat aatctgctgg ccggaactaa tgaatttatt ggtgaaggtg atgcatatat
     3361 tccgcctcat acaggtctgc cagcaaacag taccgatatt gcaccgccag atattccggc
     3421 aggcttcgtg gctgttttca acagtgatga ggcatcgtgg catctcgttg aagaccatcg
     3481 gggtaaaacg gtttatgacg tagcgtcagg ggacgcgtta tttatttctg aactcggtcc
     3541 gttaccggaa aatgttacct ggttatcgcc ggaaggggag tttcagaagt ggaacggcac
     3601 agcctgggtg aaagatgcag aagcagaaaa actgttccgg atccgggagg cggaagaaac
     3661 aaaaaacagc ctgatgcagg tagccagtga gcatattgcg ccacttcagg atgctgtaga
     3721 tctggaaatc gcaacggagg aagaaacctc attgctggaa gcctggaaaa aatatcgggt
     3781 gttgctgaac cgtgttgata catcaactgc acctgatatt gagtggccta cgaaccctgt
     3841 cagggagtaa tcattgggat tatgccgcag cacgtcttaa gcaagaacgt gctgcggttg
     3901 gatgctattt tttccctgaa gcggaaaaca ttactacagt accttgaacc ttggttttaa
     3961 cattctcgaa atgctctgag agtatatgtg ttaagccttc ttcggaatct tttgtgtttg
     4021 aaaagatgcc tttctgattg taaatgcgca tcagtttttg accgaagcta ttgtgcacaa
     4081 ctccatcgcc aagaattgt
//
LOCUS       DAJTOR010000245         4093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTOR010000245 DAJTOR010000000
VERSION     DAJTOR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.249"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(4..220)
                     /locus_tag="OO249_003949"
                     /pseudo
     CDS             complement(4..220)
                     /locus_tag="OO249_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(476..1456)
                     /locus_tag="OO249_003950"
     CDS             complement(476..1456)
                     /locus_tag="OO249_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5638055.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(1456..2655)
                     /locus_tag="OO249_003951"
     CDS             complement(1456..2655)
                     /locus_tag="OO249_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5638056.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(2977..3315)
                     /locus_tag="OO249_003952"
                     /pseudo
     CDS             complement(2977..3315)
                     /locus_tag="OO249_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3382..>4093)
                     /gene="istB"
                     /locus_tag="OO249_003953"
     CDS             complement(3382..>4093)
                     /gene="istB"
                     /locus_tag="OO249_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5638057.1"
                     /translation="AAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMYTRMAA
                     FPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAMGYE
                     AVRAGIQVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQKEA
                     KLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIKGES
                     YRLRQKRKAGVIAEANPE"
BASE COUNT         1096 a          963 c          889 g         1145 t
ORIGIN      
        1 atattacatc atggtcgagt ttcctgattt cccgggccca gtaatgagat gccccgccag
       61 cctccatcgc aacagtcatt ttaggctgtt gcaccagcca gttaagcagt tgattgcgag
      121 aaagtttggt ctttcctgcg ggtttgccat gagcattgat actgaaaaga taaaagttcg
      181 ttttagccag atcaatgccg gtgattttct gttcagacat ggtatcccgc ttacttttgt
      241 ggttaaagtt tatccatcat ggcgcaattg aaggctgccg tgaaagaggg ggaagactat
      301 ctcatcggtt actgttaaac ggctaagaga aactatcttt ttcaagcaaa gaagatacgg
      361 tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa acgctaataa aagcaaaatt
      421 tcttttaaaa cagccaatta aaaactggtg agttgctggc gaaaaacttg aattttcaca
      481 gcgaattcag attatctttt agtacaagag caatcatacg atctaactta tcctgaactt
      541 ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc ctttactcgt tttcgagcaa
      601 atttatcttt actatcaaat ttccataaca aagttacaac cgctttatcc tttactcctg
      661 aatcaagcaa tgaagacatt tccttggtta taagccgcat aatactgttt ttaacctcgt
      721 cttcagaaag attgtcatca gcagtaataa gctcaatact cggcgagata ttcttcacaa
      781 cttcatcaat agatatatca cccagcctca gtcgttcact taattcagcc agttgcctgt
      841 aatcggcgta tgtcagctca gactgaacag gaaaaagaga cacaaaatct ttaggtacgg
      901 acgctgcttg gagggcacga gtcaccttag ctgccgacaa tccctctctt tctgcaattt
      961 gcttctggtt cataccggcc tctttgagac gtactaaacg caacccaatt tcacgaatat
     1021 tatgttcaag tgacgtctgc aaatctttcg ccagatgctg agcttcagaa acagtaagct
     1081 catcatccgt tacaagtaca cgtaatccaa ccttgcatag caaagctgct gcccgacgac
     1141 gggaaccatc caaaatctca ataagatcgc cagtccgaat cccaatacat ggatagaatt
     1201 gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc cggcgtcagg gctgtctgtt
     1261 cgcggccatt cacctcttca accacaaaag tttgagtgtc cactttgtca tgatcaacct
     1321 tgatctcagt aaattttgcc tttcggccag tattgagggt aaatacatga actggagccg
     1381 atatttcttc agtattattc aggatatttg tattcagcgt acggccaata gtcggtcggt
     1441 gctttctgtt ttccatcagt agttggccct tataaattca atacgatcaa aaactgcttt
     1501 agcgaaatct tctgctgcca tacgtgcatt tttcaaagct tcaccactgc caacataagt
     1561 aacaggattc gcagatatca cggtatcaaa cgactcgccg cttctctcaa aaccatcaag
     1621 acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc tcttttgtca ggctatggca
     1681 gtatttatga tctgacttat tcgccagttt cgacatgaag ccaatatttg cctgcaaacg
     1741 gcatgaacat ccatcctgtt caataatctg tacaagttca ggtaaacgag ccaaatattt
     1801 aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt gtaaacatga tatctgcggc
     1861 agcaatggca tttttcaaaa atgcgtccag atgcgggcct gtatcaataa ggatgaagtc
     1921 gaaatcatgc ttcagcttat caataatatt ctcacgcaat atcgcgtgtt tgttttgtcc
     1981 taatagatgc tcctcgcaca gagtatccca gtttgatgca ataaaggcgt cgtcaataga
     2041 agccggcata acataaacgc cagggataac tgacggaacg ataaaatctt ccagtaattc
     2101 ctcacgactc acgttctgca acatagcctg cggtgcggtt gtatcaacca gccccaccgc
     2161 atgcaagtag ttcagaaaca tagtggctga tgactgggga tctaaatcaa ggagcagaat
     2221 acgtaaatct tctgacagca ggtgaggatg agcacgcagg gcgtgagcta cggaaacact
     2281 actcacggtt ttagacacac cgccttttag actaccgatg aatatggaat atgcctcact
     2341 ataacgatcg cgatatttag gaatgccacg atgtgcatag atatcgataa tgttctggat
     2401 ggttaatgca tattttgtcg cggttcctgc tgggcgtttt tcaaactgat agccctgagc
     2461 ctccatctca ccgactgcac catccacgct tgcgcgcgtt aattttggaa gcttagctac
     2521 agcagacttt gaatagactt gataaaaaac atcagcctga aactcctgct tttgctcctg
     2581 aatctgtttt gttagggcga gcaacatttt atctgcacgc tgcgcaacct tacttaactg
     2641 ctcaaaacta gtcattcccc ctccgtgcta tgtaggtttt taacttatta caaaaaaaac
     2701 tgcattagca gggtaatgca tgcaagtgca tattgtacat gcttatatta aaaatgcgcg
     2761 cattgctgat ttattaatca tcggaatgaa tattactgta agatagtatg cctttccggg
     2821 agaaagacgc tgatttcccc cgaataatcc agttcaccac cacactccgg acaacagctt
     2881 tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt ccggtaacgg acggcgatga
     2941 cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag tagaacatgt gcttttcaat
     3001 gaaggagccg atgacttttt cgtggatttc cacggaacgc gagaagcaga ttgttttacg
     3061 tgccagccgc ttgatgcggg tgcggagggt ccggttgtta cgttcaatac gttgcgtgaa
     3121 gattttgccg gtcaggtgtt tctccttcgg cacttctctg gcgtagctgc cccagtcatc
     3181 gctggtcacc atgccgatac agaacggagt caacagggct aacagttccc gacaggtctc
     3241 atcattccgt ggcccgaaag tgtaagccaa cacaccgcca gttttagtgt tgtacgcata
     3301 ccagagccag tgttgtgtca acgacggatg aaaagtgatc cacttatatc tccaccaacg
     3361 gcccaatatt gatccaccgt tttactcagg attagcttct gctataaccc cggcctttcg
     3421 tttctgtctg agtcgatagc tttctccttt gatttgaacg acatgtgagt ggtgtaagat
     3481 acggcccagc atcgctgagg tcagtgctgc atcaccggcg aacgtttgat cccactgccc
     3541 gaacggtaga ttggatgtca ggatcattgc gctcttttca taacgtttag cgatgacctg
     3601 gaagaacagt tttgcttctt tctgactgaa cggcagatag cctatttcat caatgatgag
     3661 caggcggggg gccattactc cacgctgaag cgtcgtttta taacggccct gacgttgtgc
     3721 cgtagataac tgaagtaaca gatctgctgc tgttgtgaag cgaacttgga tacctgcacg
     3781 gactgcttca tagcccatcg cgattgccag atgggttttc cccacacctg atggccccag
     3841 taatacgata ttttcattac gttctatgaa gctgagtgag cgtaacgact ggagttgctt
     3901 ctgcggtgct ccggtggcga atgtgaagtc atactcttcg aacgttttca ccgccgggaa
     3961 ggctgccatt cgggtataca tcgcctgttt acgttgatga cgtgccagtt tttcttcatg
     4021 aagcagatgc tccaggaagt ccatataact ccattcctgg tctactgcct gttgtgacag
     4081 cgcaggcgct gcg
//
LOCUS       DAJTOR010000246         4088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTOR010000246 DAJTOR010000000
VERSION     DAJTOR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.250"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..178
                     /locus_tag="OO249_003954"
     CDS             <1..178
                     /locus_tag="OO249_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5638058.1"
                     /translation="HDVLTGSDDGRHSNTSLAWGAGVQFNPTESVAIDIAYEGSGSGD
                     WRTDGFIVGVGYKF"
     gene            243..1650
                     /locus_tag="OO249_003955"
                     /pseudo
     CDS             243..1650
                     /locus_tag="OO249_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422246.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            1736..2248
                     /locus_tag="OO249_003956"
     CDS             1736..2248
                     /locus_tag="OO249_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638059.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            2324..2536
                     /locus_tag="OO249_003957"
     CDS             2324..2536
                     /locus_tag="OO249_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5638060.1"
                     /translation="MPEKFFRTDADNNDVPMTAASWMALSEATEQAMFAKGVEINTRQ
                     LQMKAEVEALTDLKAIRSYVVGWPAG"
     gene            complement(2678..3003)
                     /locus_tag="OO249_003958"
                     /pseudo
     CDS             complement(2678..3003)
                     /locus_tag="OO249_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(3139..3387)
                     /locus_tag="OO249_003959"
     CDS             complement(3139..3387)
                     /locus_tag="OO249_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5638061.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(3742..>4088)
                     /locus_tag="OO249_003960"
     CDS             complement(3742..>4088)
                     /locus_tag="OO249_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5638062.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT         1093 a          879 c         1148 g          968 t
ORIGIN      
        1 gcacgacgtg ctgaccggaa gtgatgacgg tcgccacagc aacacgtctc tggcgtgggg
       61 ggctggcgtg cagtttaacc cgaccgaatc cgtggccatt gatattgctt atgaaggttc
      121 cggcagtggc gactggcgca ctgacggttt catcgtgggt gtcggctata aattctgatt
      181 agccaggtaa cacagtgtca tgacagcccg ccggttcagg cgggcttttt tgtggggtga
      241 atatgacagt aaagatttct ggtgtactga aagacggcac aggaaaaccg gtacagaact
      301 gcacaatcca gctgaaagca aaacgtaaca gtaccacggt ggtggtgaac acgctggcct
      361 cagaaaatcc ggatgaagcc gggcgttaca gtatggacgt tgagtatggt cagtacagcg
      421 ttattctgtt ggtggaggga ttcccgccgt cacatgccgg gactatcacc gtgtatgaag
      481 attctcaacc cggtacgctg aatgattttc tcggtgccat gactgaggat gatgcccgtc
      541 cggaggcact gcgtcgcttt gaactgatgg tggaagaggt ggtgcgtaac gcagaggagg
      601 cgaagaagaa tgccggagag gcggagacgt cagcgaggaa tgccggcata tcagccagtc
      661 aggcagaaga gagcgctgca aatgctgaca cttcagcagg ggatgcatcg gagtcagccc
      721 ggcaggcggc agaaagtgca gcctcagcaa agcagtcaga ggaggcgtcc tcgtcctcgg
      781 cctctgcggc cgctcaaaaa gccagtgagt cattacaaag tgcaacagat gctgagttgt
      841 caaaaaagac ggcagaaagt gcagccggta atgcagtcag ggatgcaacg accgcaacag
      901 aaaaagcccg ggagtcagca gaaagcgcac agtcagcgga acaaagcagg atagcggcgg
      961 aagaggccgt aaaccgaatc cccaccgtgg tgggacctcc cgggccaaag ggggaaccgg
     1021 ggcccgcggg tcctcagggg ccgaagggag ataaaggaga gcgtggcgac accggcccgg
     1081 caggggccaa aaggtgacaa aggcgatccg ggagtggctg gaccagcagg tccggcaggt
     1141 gcgccggggc cgaaaggcga taaaggtgat ccgggagtag caggtccagc aggtccggcg
     1201 gggccacagg gaccgaaggg agacacagga gcccccgggc aaggaacaga actgcttact
     1261 actgccaata catggactca ggcacaaact tttaatggtg gtattaatgg caatttgacg
     1321 gtgaccggaa acggctcatt taacgatatt cagatccgtt cggataaacg taacaagcga
     1381 aatctggtaa aactggataa tgcgttagat cgtctggagg cacttactgg ttatctttac
     1441 gagatacagt actctgccga cggttggcaa acgtcggttg gtttaattgc tcaggatgca
     1501 caaaaagcat tgcctgaact ggtaactgaa gacgcagacg ttatatctgg tgaaaaacgt
     1561 ctgcgtctta actacaacgg cataattgca ttgttagtcg agggctttaa aacacttcgt
     1621 catgagatta aagaactccg ggagaagtaa acgacagctg ttgtagtttc tggtttctac
     1681 tgaatttaaa ttgtggggtg acactcaccc cacgcattca gaagggggag atgagatggg
     1741 ggtaacatcg ggatgggtag gctcttcggc taagagcgaa acaggtgagc agtggatggg
     1801 agccgccgga accaaactgg gactgagtaa accttttatg atgagccaaa tggttgggcg
     1861 aactatgggc tgtaaaattg caactgagta ctataaatgg aaatcctctg acaaggttga
     1921 taactggggc gcagttggcg ctgactggcc tttagaagaa aaaagtaaag gtacaattac
     1981 aaacgccgca agctgcggat cagggaggct ggtaggggct gtcgttacac tttctcactt
     2041 tttgacgaac tctacaccga cagctgctgt ttatttagcc ggtggtaaag caggtaacat
     2101 caccgtaaac gtaggtggtg ctacacaaac catgatttat cagggcgttg ttagtgggtt
     2161 ccagtattac tggtcaggtt ctgttagttc cgctttcgtg gaggcaatga aaaagacggg
     2221 agtaccccag gatctaaaaa ttagttaaat ggtaaataaa taattttaaa aacttcacgt
     2281 tctacatacc ggagacgccg gatatatagg atattgtttt aagttgccag agaaattttt
     2341 ccggacggat gctgataata atgatgtgcc aatgacggca gcatcatgga tggcactgag
     2401 tgaagcgaca gagcaggcga tgtttgcgaa gggagtggag attaatacac ggcagttgca
     2461 gatgaaagcc gaggttgagg cgctgacaga cctgaaggcg atccgcagtt atgttgtcgg
     2521 atggcctgct ggctgaataa aaaaacggga ccacgaccag tcccggaacc atgagtttta
     2581 gggtattagt ttgttatcat agttagcgtg ctaagtatgc catgtcaggt tgattagtga
     2641 agtgatgttg ttcgcatttt tgcacggcgg agaatattca gatttttgga aatccatatt
     2701 tttcccgtgc gcgattacat gcttcattta cgatgctatt ttcgccggac attgcgaatt
     2761 ccctgcatgt ggacggtttt tgtaaacaga gcaatatgcg ttttctccgg ggggggccgg
     2821 caagagcgcc acatcgggga tttttctgat tggtgccgcg catacagcga tgaaaaggag
     2881 atatttgctc agtgagactg accgaaacct ttccgccagc atcgtcagct tctgcccagt
     2941 aaaaagagac gcggaaaatg cacaacaggc accacacgtc atgcatggat tcagattgct
     3001 cataattcac cagtacagct ataaatcgta aagagaacag cggtacatcg tatgtaagaa
     3061 cgtaccgcgg ctggctggtg aacttccgat agtgcgagta ttgaatgatt tccagccgtt
     3121 accgatttta cgtgctaatt agtgaacaaa ccactcgtca gcagactccc aggtatcttt
     3181 cagagtttcc tgaacaaaag ttttagctga atctttatcg gcggtgcgcg taacagaaag
     3241 gccatcgttg ctggtagctt ttacgatcac ctctacatcg tcataacgtt tactgatgcg
     3301 tcgggttaat tcttccttta acgcatccac agcaccggtt ggcattttag tcattttttc
     3361 tttggctatg cagatttcaa tacgcataaa agtccctcta cactgtgttt gtatacagta
     3421 tcatttttaa ctgtatggat aaacagtatt aagggtgaag gaaagtgatt ttttggggca
     3481 tgcatggggc aaaaatattt gttgtggggc attttggggc atgattggga cactttgtta
     3541 catatgaact ttgccgattt ttatatgaca tggaaataca acgctttgat tcttctaaat
     3601 aaaaaacatg ctcttgggcg ttctttagtg atttttaaaa attccgtatc acgcagttaa
     3661 agtggcgggc atactcttca aggctggtga tccccaaccg cacccattta ggatgcgacc
     3721 attgcggtag accgatgtaa atcataacgc gtgaaggatc tcttccacgc tacgcacacg
     3781 ggcgatgcgc gggtagatat gattaatgct gttattgtgt tgctcggcgc tggcggcact
     3841 acaggcgtct tcggcaatca ccagattaaa gccaagttcc caggcattgc gggcggtgga
     3901 ttcaacaccg atattggtcg agatcccaca taacactatt gtatcgatac ctcggcggcg
     3961 cagttgcaac tccagatccg taccgtaaaa cgcaccccac tgacgtttga tgatttcgat
     4021 atcactgtca gttgcaccta atgcagctgg atgttgccac cagttttcgg gcaacacttt
     4081 agcggggg
//
LOCUS       DAJTOR010000247         4078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTOR010000247 DAJTOR010000000
VERSION     DAJTOR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.251"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..1046)
                     /locus_tag="OO249_003961"
     CDS             complement(<1..1046)
                     /locus_tag="OO249_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5638063.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1647
                     /locus_tag="OO249_003962"
     CDS             1537..1647
                     /locus_tag="OO249_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5638064.1"
                     /translation="MLIGYVRVSTNDQNTDLRRNALNCAGYELIVERMGA"
     gene            1927..2922
                     /gene="pgl"
                     /locus_tag="OO249_003963"
     CDS             1927..2922
                     /gene="pgl"
                     /locus_tag="OO249_003963"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5638065.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2963..3915)
                     /locus_tag="OO249_003964"
                     /pseudo
     CDS             complement(2963..3915)
                     /locus_tag="OO249_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1094 a          828 c          957 g         1199 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctatcc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atatcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattat cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaacac cgatctacga cgtaatgcgt tgaactgtgc aggatatgag
     1621 ctgattgttg agcgaatggg agcttgattg gaagttgtta gggtatggtg tttacggtcg
     1681 ttgatatggc gaacaaacgc ggggagcgtc acgctcccta aatggtcgcg aaaatatctg
     1741 tggcattgtc aaatttgtga cggtgactaa tattttaatg atatgccatt cgaaatggtg
     1801 tgctaacgta ttgatttaat tgtgacgcga tgttgtattt tatcgcgttt tgttgtctgt
     1861 ttttcattcg cttgatgttg ctatttacat gaaaaataaa gaatttattg tttttgaggt
     1921 gtgtttatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg
     1981 aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg
     2041 cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt
     2101 cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac tgacctttgc cgcagagtct
     2161 gcgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta
     2221 ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc
     2281 gtcgtcgatg tggtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac
     2341 cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat
     2401 gatggtcatc tggtggcgca ggaccctgcg gaagtgacta ccattgaagg ggccggcccg
     2461 cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc
     2521 tcagtggatg tctgggaact gaaagatccg cacggtaata tcgaatgtgt ccagacgctg
     2581 gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca tatcaccccg
     2641 gatggtcgcc atttatacgc ctgcgaccgt accgccagcc tgattaccgt tttcagcgtt
     2701 tcggaagatg gcagtgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc
     2761 ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa atctcatctc
     2821 atctcggtat acgaaattgt tggcgagcag ggactactgc atgaaaaagg ccgttatgcg
     2881 gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttaaccggcg
     2941 cagtctctcc tgcgccggtg tattaaccta tctcctgtaa cgcatgtctc tggcgttcga
     3001 cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc aaggcaaaaa
     3061 agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc aatgtccgac
     3121 gtataacggg ttccaccagt gggataccaa taatcgaaga atagggcgca tggggcattg
     3181 ccagaccagg aagcgccgaa atccccagtc ctgcctccac tagtcctaat gacgtcgaaa
     3241 gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcggccagc tgttgttcta
     3301 tcaacagtcg gttgccgctg gaggagcgta cgccaatcat tttgtaaccc actaactcct
     3361 gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggca gcacgaacgg
     3421 ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg acgttgttca tgtttatgcc
     3481 aaagtctgat tcgttacaca gcaccgattc catgcaatta ttcgtgcctt gttccagaat
     3541 ccgcacttta atattgggat acagctcatt aaatttaccg attgccagcg gtaaaaaata
     3601 aaatactgcg gtcggaatac acgctaacgt caccatacca cgatgataag cattcatatc
     3661 acgaatatta aaaagcgttt catcgaattt ctttattaat tcccgcgcct ccgggagcaa
     3721 cctttttcct gcttttgtta acgtaacctt gcgagttgta cgctcaaata gttgaatatg
     3781 taaatcttcc tccatctttt ttattcttcg cgttaatgca ggttgcgtaa tattgagtaa
     3841 tttagcggca ttgttaaacg aactggactc tgccagtatg acaaatgcct tcatacttga
     3901 taattcatgc ttcattatta ctccggaaaa tggaagcgac gattttgggt ggctggccgt
     3961 taaaaatttt aactgcattt agccaactta aattaatgaa aaaatgttat taatcgttga
     4021 gctaaagtca ttagagatgc tttgccctta atgtaaccat atcgcaataa gggtaatg
//
LOCUS       DAJTOR010000248         3945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTOR010000248 DAJTOR010000000
VERSION     DAJTOR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.252"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..1627)
                     /gene="gcl"
                     /locus_tag="OO249_003965"
     CDS             complement(<1..1627)
                     /gene="gcl"
                     /locus_tag="OO249_003965"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5638066.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKTVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTYFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALM"
     gene            complement(1717..2532)
                     /gene="allR"
                     /locus_tag="OO249_003966"
     CDS             complement(1717..2532)
                     /gene="allR"
                     /locus_tag="OO249_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5638067.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(2610..3092)
                     /gene="allA"
                     /locus_tag="OO249_003967"
     CDS             complement(2610..3092)
                     /gene="allA"
                     /locus_tag="OO249_003967"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5638068.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3322..>3945
                     /gene="allS"
                     /locus_tag="OO249_003968"
     CDS             3322..>3945
                     /gene="allS"
                     /locus_tag="OO249_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5638069.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTK"
BASE COUNT          997 a         1074 c          953 g          921 t
ORIGIN      
        1 ccattaaggc tttcgcctgt tcaaaggctg gcgcaatatc ttccggtttg aagacgcgaa
       61 tcgctttaca acctaaacct tccgctactt ttacgtggtc gacgccgtaa ccgttcactt
      121 cgctggagtt gatattctcg aaagcgagtt gcacgcagta gtccatgtca aaggcgcgtt
      181 gtgactgacg aatcaggccg agataggcgt tattcaccag cacatggatg tacggaatgt
      241 tgaactgcgc gccaacggct aactcttcaa tcaggaactg gaagtcaaag tcgccagaaa
      301 tcgccaccac attgcgtttc ggatcagcgg cacaaacccc tagcgcagcc ggaatcgtcc
      361 agcctaacgg accagcctga ccacagttga tccagtggcg gtctttaaag acatgcagca
      421 tttgtgccgc agcgatttgt gacagaccga tggtggtgac ataacaaaca tcgcgaccaa
      481 aggctttgtt catctcttca tacacgcgct gcggtttcac cggcacgttg tcgaagtagg
      541 ttttgcgcag caaagtgcgt ttgcgctgct ggcagtcggc gacccattct ttacgacacg
      601 gcagacgacc cgctttttgc atctcctgcg ccacttcaac cagcagtgtc agcgccgctt
      661 tagcatcaga gacaatgccg agatccggac acagcacgcg accaatttgc gtcggctcaa
      721 tatcgatatg aacgattttg cgcccttcgg tgtatttctc taccgaacca gtatgacggt
      781 tagcaaaacg gttaccgata ccaaacacca tgtcggacgc cagcaacgtt gcgttaccgt
      841 aacgatgcgc ggtttgcaga ccgaccatcc cggccatcag ttcatgatcg tccgggatac
      901 agccccagcc catcagcgtt gggatcaccg gaacgctggt cagttcagca aactgttgta
      961 acagtgcagc tgcgtcagca ttgattaccc cgcccccggc aacaatcacc ggacgttcgg
     1021 cctggattaa catttctaca gttttttcga tctgcatacg gctggcagca ggtttgtaga
     1081 ccggcagcgg ttcgtacatg tcaggatcaa actcgatttc cgcaacctga acgtcgaacg
     1141 gtaaatccac cagtaccgga cccggacgac cagaacgcat caggtgaaat gcctgttgca
     1201 gcacgcgagg caccagcgcc gcttcacgaa ctgtaaccgc cattttgctg accggtttag
     1261 caattgcttc aatatctacg gcctgaaaat cttctttatg cagacgggcg cgcggtgcct
     1321 ggccggtaat gcacaaaata ggaatggaat cagcagaagc ggaatagagc gcggtgatca
     1381 tgtccgtgcc cgcaggaccg gaagtcccca gacatacgcc gatattccct gccgttgcgc
     1441 gggtataacc ttccgccatg tgcgaagcac cttccacatg acgcgccaga atgtgacgaa
     1501 taccgccgtg cttacgcatc gctgagtaga acggattgat tgcagctccc ggaacaccga
     1561 aggcggtggt gataccttct ttctccagca catacattgc cgcgtcaacg gctctcattt
     1621 ttgccatttt attcctatct ctatttattg gaaaattttt ccaactttca aattttgtca
     1681 aaaaaacccg ctccgcagag cgggatgcga cagacattat ggatgtgctt tcagtcccaa
     1741 cgccgtgctg atatcgcggg cggtgtctct gaccagctca ccctgactga caaaacgatc
     1801 ttctgtcagt cttgatgaag gcccggagat ggagatagcg gcaacaacgc taccgacatc
     1861 atcgtaaatt gctgaagcta tgcaattcag acctacaaca tgctcttctt tatctacggt
     1921 atagcccagt tcacgcgctt gttccaggtc cttcagcaag gtgggcatat ccacaagcgt
     1981 agttggcgta aactgctgca aaccggtttg cagaatgatg ctcatcaact cctcttccgc
     2041 cagcggataa agcagcgctt tgcccgcacc ggaagcatgc agcggcagac gactgcccag
     2101 tggcgcacac atcctgacca ttgatttaca ctctaactga ccaattaata ctgcttcatt
     2161 gccgttacgg atcgcgacat tgaccgtttc gccggaaagt aacatcaggc ggcgcataaa
     2221 cggcccggcg acggagagga catcgcggtt atggatgtac gccgcaccga cgttaaagac
     2281 acctaatcct atatgccacc agcctaattg actgtcctga tagacaaaat ccgctgcttg
     2341 taaaaccttc agcaagcgaa aggtcgtgga gagcggcaaa tccagattga gagaaatatc
     2401 gctaaccgac gaacttcccc cacttttttc cagatattgc agaatcgcaa ttccccgctc
     2461 taacgcctgt gcgcccttct gtgccacagg ctccgcctgt cctggcctgc cgcgccgtct
     2521 aacttccgtc atacctacgt taactccgac ctacaagtga gcataaacaa tgaacaatta
     2581 gtttatctta tgcaagtcgg ttgcaggcgt cacgcaaaac agagttcctg ttcaggaata
     2641 ctttcaacat cacagttgtc actgccgccg cgatcgatgg tcagaaaatc ggtgacgcgc
     2701 tgccaggcga aaagtgggtg atgccagacg ttacgatggt aattcactcc ctgttcgccg
     2761 ttggtgataa acgcccgcag cgttgacagg tctggtttgt cgtcgcctaa cgccacgacc
     2821 accacaaaca cttcaccttt catcgggata aaggcctgag tgcccagcgg atgacgttcg
     2881 agttcgtgaa tggtcagcgg cagattcgcc ggttgcgcgc ggttaatgct aataagcgta
     2941 cggtcttgct caagaatctc aaccagcgcc aaatcgtggt aacgctccac caggccattg
     3001 ttaatatgga aaaaatcccg ttgctgagtt tcgattacgt cgccataagc actaaaggct
     3061 tcctgactta acggtaatac ctgaagtttc atctttttgt ccttgtaaca taattccgtt
     3121 caaccaccgc cgaccggtca gcttgggttc aataacaggc aatctaaaac cttaagagaa
     3181 caacatcaat acaaaagttg gaatattttt ccaaaactag atttagtcca caaattgaac
     3241 agggttatca tgtaaaaata ctgataaatc agaggataag aaaatcacgc aacttgcgga
     3301 tttaaaaggg gatacctgaa gatgttcgat ccagaaacct tgcggacttt cattgcggtt
     3361 gctgaaacag gaagtttttc aaaagcggca gaacgattat gtaaaaccac ggcgacgatc
     3421 agttatcgca ttaaacttct ggaagagaat accggagtag cgctgttttt ccgtacgact
     3481 cgtagcgtaa cgttgacagc ggctggcgag catctacttt cccaggccag agactggctg
     3541 agctggctgg aaagtatgcc cagcgagctg caacaggtga atgatggcgt ggaacgccag
     3601 gtgaatattg tcatcaacaa cctgctctat aacccccagg ccgtcgccca gttgctggcg
     3661 tggctgaatg aacgttaccc ctttacccag tttcacatct cccgacaaat ctatatgggc
     3721 gtctgggact cgctattgta cgaaggtttt tcgctggcta tcggcgtcac gggaactgag
     3781 gcgctggcaa atacctttag tcttgatccc ttaggatcgg tgcaatggcg atttgtcatg
     3841 gcggcggatc atccgctggc gaacgttgaa gagccgctaa cagaagcgca gttgcgtcgc
     3901 tttccggcgg tcaatattga agacagcgcc cgcaccttaa ccaaa
//
LOCUS       DAJTOR010000249         3921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTOR010000249 DAJTOR010000000
VERSION     DAJTOR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.253"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..266
                     /locus_tag="OO249_003969"
                     /pseudo
     CDS             1..266
                     /locus_tag="OO249_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(320..474)
                     /locus_tag="OO249_003970"
                     /pseudo
     CDS             complement(320..474)
                     /locus_tag="OO249_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(557..1735)
                     /gene="ltrA"
                     /locus_tag="OO249_003971"
     CDS             complement(557..1735)
                     /gene="ltrA"
                     /locus_tag="OO249_003971"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5638070.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVKHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNTIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            complement(1769..2254)
                     /locus_tag="OO249_003972"
     CDS             complement(1769..2254)
                     /locus_tag="OO249_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5638071.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            complement(2242..2526)
                     /locus_tag="OO249_003973"
     CDS             complement(2242..2526)
                     /locus_tag="OO249_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5638072.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            3199..3921
                     /locus_tag="OO249_003974"
                     /pseudo
     CDS             3199..3921
                     /locus_tag="OO249_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1013 a         1033 c          893 g          982 t
ORIGIN      
        1 tattgcttcc ggcacaacat gttaaagctt atcagcggtg ccagaaaaat gattataacg
       61 acgcacaggc aatagctgag gcctgccaac atggaactat acgacccgta ccaataaaaa
      121 ctctggagca acaggacgtc caaacttttt tgaatatgcg tcggttggtt tcaatggaaa
      181 ggacacaact gataaatcac attcgtggac ttcttgctga atacggcatt gtgttcagta
      241 aaggcgcagc agaattacgc caaaagtaag cggctcgtca gaaccgtatt gatatttact
      301 gagagctcag atcaactttc tagggcaaca gatcacgtac ccggtttgcc ggccagtcct
      361 ggatatgctc aatgacgtaa cgcagccatt ttctggttcc acattgttca gacggcatgt
      421 gccgatcagc gagtacaaca ccgccgcatg ttcgtcatcg ctgtcggaac ccgcgagtcg
      481 agtcagccgg agagaatttc actccccggc tgctcacgga accgtacgtg acactctcgc
      541 gtcatacggc tcttgatcac tcatttgatg cacgtagtaa ataccagtga acaaatagct
      601 tcggatactc cttccttacc agcgtcagct ttcgctgagc cttccggttc gaaagtctgt
      661 atttagactg catccacttg agcagacgtg actgcattgc actccacagt cgatagttga
      721 aatttctgga ccagaacttt ccgtagtact cgatccagcc tctcactatc gtattgtagc
      781 gcctcgcaaa atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg
      841 tttctgttat tttgcgcatt gctgcatttg acgcacccgg catgcatttt cggtaccgtt
      901 cgcctttgaa attcttcagc gtacgcactt tgaagtcata tccgagaaag gtgaaactcg
      961 ttgcgacgtt tctcctttta aacgtgtcaa tgtaggcgat attcgttttc cctgcattca
     1021 ggaccagccc gacttccgag aagcgctccg ataacctgtt ctttagcctt gttgcatcac
     1081 tcattcgtga acaatgtact acaatatcgt cagcgtacct ctcaaacggt accccccgat
     1141 attctctttc catccacaaa tcaaatgcat agtgaaggaa caagttagca agtagcggac
     1201 taatgacccc gccctgcgga gtgcctcgtg ttcgggttat taattctcca ttttcacaac
     1261 tctgcattgg cgcttccatc cagcgtcgac agtacaggat gacccattta ggcatcccgt
     1321 ggtgttccag agctttgagc accagatcat gtttcacatg gtcgaaaaac gcactgatgt
     1381 ccacttccag tatccagcta taacgccagc accggatggc acattgtttc agagcgtcat
     1441 gagctgattt gcctggtcgg taaccatatg aatcagcatg gaaaatcggg tcaagctttt
     1501 cttccatgaa aagttttatc gctccctgag caattcgatc ggatactgtt gggatcccca
     1561 gaatgcgttc cttgccattc gacttcggga tccgtttttc cagcactggt ggcggaaacc
     1621 atgttcccga acatagccgg ttccagatct tgtatagatt gccatcacgt tgctggtcga
     1681 acattttcaa agtctgcccg tcgcatccgg ggggctccct tgttgcgacg cacatccaga
     1741 tatgatgccc agaccagtgc attactccct attgagggag tttaagaaac aatgtttgca
     1801 cctgagtttt tgatggctta aaaccatgat gaatataaaa ctgttttgcg ttttccgtaa
     1861 gcgcatgaac cataattgca cgcacaccga tattttcggc aaccctgtaa caacgacgaa
     1921 ctgcatcatg aagtaaatcc gcgccaagcc cttttcctcg aaatgacaca tctacagcca
     1981 ggcgtgcaag tataattaca ggaatggggt ctggcatatt gcgccgaagg ttacctgtag
     2041 cttctgtatg attgacacta ccagtggcca gtgagtaaaa accgactatt tgttgcgtac
     2101 cttttctgca tacaacaaaa gtccgggtgg ctccgattgc ctgatttttt agccccttct
     2161 gctttaacca gtcatcaagt acagcttcac cgctgacaaa ttcagcgacc tgatgaaatg
     2221 aggataaagg ttctggtgca gttacacatc ccattgaggt ttccttgcca gcaatttttc
     2281 gatggcagga tcatctgcaa ccggagcatc aagcatttca atgaactctt catattgcct
     2341 gtcattaaaa ttaaacaccc gccggtcgag aataacatct tcagcagctt tacaggccat
     2401 ttccagaatg aaatctgtac gcgacttatg aagaatttct gcagcagcat caatgagtat
     2461 ccgctgtgat tctcttgctc tgagattgag ctgaacacct gatttcataa agcctccgta
     2521 tatcatttgc tttacaacaa tagcatattg acaatatata gcaaatgatg tacacaaagt
     2581 aacagattac agatgccgcg tcagcggcat ttacctctgt ggacgtaacc gcactcaaag
     2641 gggatatcgg gtggcgcagg ggcaccgccc ccctacgctc ccacggaacc gtacgtgaca
     2701 ctctcgcgtc atacggctct tgtcgttgaa ccattcaagt atcgtaagct aagcaccagt
     2761 gtgcaaacag cgtcgggcag tcgtgacgaa ttcgcagtaa ccactcgaat gcacgcacca
     2821 gactacctcg cgctcgcttg tgtttgcggg caatccactt ggccaagtgc aggtcaacct
     2881 gcgccattac cgggtatagc ttcgattggt aacctcaaac gcctcgactg acccccttcg
     2941 ctccatgccc attacaggca cttcctcact actacgaagc catctgcccc tgtgtctcgc
     3001 atcggtactc aggcgcttgc agtgcctctg ctccgcgccg ctcccttagc atcgagacga
     3061 caggttccca cgttccgtac gagagcctgt gttgcgttca tgctgcctat atgccggatg
     3121 ccgccctgaa gtggcacact gaatttggcc acctgaacag aggtgatatg ctcacctcag
     3181 aacaacacag gtgctccaat gaaaaaaaga aattttagcg cagagtttaa acgcgaatcc
     3241 gctcaactgg ttgttgacca gaaatacacg gtggcagatg ccgccaaagc tatggatgtt
     3301 ggcctttcca caatgacaag atgggtcaaa caactgcgtg atgagcgtca gggcaaaaca
     3361 ccaaaagcct ctccgataac accagaacaa atcgaaatac gtaagctgag gaaaaagcta
     3421 caacgcattg aaatggagaa tgaaatatta aaaaaggcta ccgcgctctt gatgtcagac
     3481 tccctgaaca gttctcgata atcgggaaac tcagagcgca ttatcctgtg gtcacactct
     3541 gccaagtgtt cggggttcat cgcagcagct acagatactg gaaaaaccgt cctgaaaaac
     3601 cagacggcag acgggctgta ttacgcagtc aggtacttga gctacatggc atcagccatg
     3661 gttcggccgg agcaagaagc atcgccacaa tggcaacccg gagaggctac cagatgggac
     3721 gctggcttgc tggcaggctc atgaaagagc tggggctggt cagctgtcag cagccgactc
     3781 accggtataa acgtggtggt catgaacatg ttgctatccc taactacctt gaaaggcagt
     3841 tcgccgtgac cgagccaaat caggtgtggt gcggtgatgt gacctgtatc tggacgggta
     3901 agcgctgggc gtacctcgcc g
//
LOCUS       DAJTOR010000250         3909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTOR010000250 DAJTOR010000000
VERSION     DAJTOR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.254"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..760
                     /gene="pgrR"
                     /locus_tag="OO249_003975"
     CDS             <1..760
                     /gene="pgrR"
                     /locus_tag="OO249_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5638073.1"
                     /translation="LRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNR
                     PSGTIRITTVEHAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGG
                     EMDKDMIAIRIGPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWR
                     LIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKF
                     TPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            1097..2710
                     /gene="mppA"
                     /locus_tag="OO249_003976"
     CDS             1097..2710
                     /gene="mppA"
                     /locus_tag="OO249_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5638074.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMKQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRIMYIVKH"
     gene            complement(2761..3792)
                     /gene="ynaI"
                     /locus_tag="OO249_003977"
     CDS             complement(2761..3792)
                     /gene="ynaI"
                     /locus_tag="OO249_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5638075.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1100 a          863 c          908 g         1038 t
ORIGIN      
        1 attgcggctt ctgacgcgaa ccacgcgcag cgttgttcca actgaagcgg gcgagcatct
       61 tttgtctgtt cttggcccta tgttgcatga catagattca gccctggcat ccctgagcga
      121 gctgcagaac cgcccatccg ggacaatacg tattactact gtagaacatg cagcaaaaac
      181 gatattgtta ccagcaatgc gcacattcct gaaatcgcat cctgaaattg atattcagct
      241 caccattgat tatggtttga ccgatgtcgt ttctgaacgt tttgatgcag gcgtccgtct
      301 gggtggggag atggataaag atatgatcgc cattcgaatc gggccagata taccaatggc
      361 tattgttggc tcaccggatt atttttctcg ccgaagtgtt ccaacgtcag tgtcacaatt
      421 aatagatcat caggcaatta atttgtatct tcccacatcg ggtacagcaa atcgctggag
      481 attaatacgc ggtggacgtg aagttcgtgt tcgcatggaa ggtcagcttt tactgaatac
      541 gatagacctg atcattgatg ctgcaattga tgggcatgga ttggcgtatc taccttatga
      601 tcaggttgag cgggctatta aagaaaaaaa actgatacgt gttttggata aattcacacc
      661 agatttaccc ggttatcacc tgtactatcc acaccgtcga catgctggct cggcattctc
      721 attatttata gataggctga agtataaagg tgctgtttag cactacttgc tgatacatta
      781 atttaatttc tctcttaacg tattctcagt tcctttcaac gttttggtta ttttttattc
      841 ttcgtacaat ggcgacagat gctgattttg ataccgaaaa ctggtttgaa cgtgcgaagc
      901 ctgaacgtag tattcggtgt tctatgtgct ttgccgcatt ttggcgtcga ttatttacac
      961 taaatctgat ttaatatatt gatacataaa acattttatg cttcctgtgt cacttttttg
     1021 atggaagttg tttgcatttc tttaaggcga aacaaataat tacgcatcaa ttttaatgtc
     1081 ggttagaggg aaacttatga agcactctgt ttcagtcacg tgttgtgcgc tgttggtcag
     1141 cagcatttct ctttcgtatg ctgcagaagt tccgagcggc acagtactgg cagagaagca
     1201 ggagctggtg cgccacatta aagatgagcc tgcgtcgctg gatcccgcta aagccgtggg
     1261 cctgccagag attcaggtca ttcgcgatct gtttgaagga ctggtgaatc agaacgagaa
     1321 aggggagatt gtccccggcg ttgcgactca gtggaaaagt aatgacaacc gtatctggac
     1381 ttttaccctg cgcgataacg caaaatgggc ggatggcaca ccggtaacgg cgcaagattt
     1441 tgtctacagc tggcagcgtc tggtggaccc gaaaacgcta tcgccatttg catggtttgc
     1501 cgcgctggcg ggaatcaaca acgcacaggc gattattgat ggtaaagcta cgcctgacca
     1561 gcttggcgtc accgcagttg atgcccatac cttgaaaatt cagcttgata aaccgttgcc
     1621 gtggtttgtg aatttaaccg ctaactttgc cttcttcccg gtgcaaaaag ccaacgtaga
     1681 aagcggtaaa gagtggacga aacccggaaa tctgatcggc aatggcgctt atgttcttaa
     1741 agaacgcgta gtcaatgaaa aactggtcgt ggtaccgaat acccattatt gggataacgc
     1801 caaaacggta ctgcaaaaag tgaccttcct gccaattaat caggaatccg cagccactaa
     1861 gcgttacctc gcgggggata ttgatatcac cgaatccttc ccgaaaaata tgtatcagaa
     1921 gctgttgaag gatattccgg ggcaggttta tacgccgccg cagctcggga cctattatta
     1981 tgcgtttaac acgcaaaaag ggccgacggc agatcagcgc gttcgcctgg cattaagtat
     2041 gacgatagat cgccgcctga tgaccgaaaa agtgttggga acgggcgaaa agccagcgtg
     2101 gcattttaca ccagatgtta ccgccggatt tacgccggaa ccttcgccgt ttgaacaaat
     2161 gagtcaggaa gaactgaatg cgcaggcaaa aactttgttg agcgcagctg gttatggtcc
     2221 gcaaaaaccg ctgaagctga cgcttttgta taacacttca gaaaaccatc aaaaaattgc
     2281 gattgctgta gcatcgatgt ggaaaaagaa ccttggcgta gatgttaaat tgcaaaatca
     2341 ggaatggaaa acctatatcg atagccgtaa caccggcaat tttgatgtta tccgcgcctc
     2401 atgggtgggg gattataatg aaccctccac tttcctgaca ttattaacgt caacgcattc
     2461 aggaaatatt tcacgcttta acaatccggc atacgacaaa gttctggccc aggcatcgac
     2521 ggaaaatacc gttaaagcgc gtaatgccga ttacaacgcg gcagaaaaaa tcctcatgaa
     2581 gcaagcaccg attgcaccaa tttatcaata taccaatgga cgattaatca agccgtggct
     2641 gaaaggttat cctattaata acccggaaga tgtggcgtac agccggatta tgtatattgt
     2701 gaagcattga tgtgatggga actggcgtta cccttgtgga taacgccagt gatgtctgat
     2761 ttagcgcccc tgttccggcg gtgtaatgtt atccatatac agcgtctggc tggggaaggc
     2821 aaagtccgcg ccgtgtgact gcacaatatc gataatcttc aaataaacgt cttgttgtgc
     2881 agcaagccat tcagcccata ccgtggtttt ggtaaagcaa taaaccataa tattcaatga
     2941 cgagtcggca aactggttga aataaaccag taaggtttgt cgctggtcga tggccgggtg
     3001 atttttcagc atctcacgta cggcttcgac aataacgccc acttttgccg catcctcata
     3061 acgtaaacca atggtcgtgg taatgcggcg gttggtcatt cgtcctgggt tttctacgct
     3121 gatcgacgaa aacagcgagt tcggtacgta caatggacga ttatcaaagg tcgtaatttt
     3181 ggtaattcgc cagccaattt ccgctactgt accttcgata tttctgtccg gtgaacggat
     3241 ccagtcgcca atactgaaag gacggtcgaa atagagcatg atcccggaaa agaaattact
     3301 cagaatatct ttaccggcca taccgacagc cagaccacca ataccaccaa aggtcagcaa
     3361 gccagaaagg ctcatgccga aatgttcgcc ataaagcaga acaagcacca caataatggt
     3421 gattttgata atgcgcgaca taatccgcgc actggtgata tcgcgacctt ttttaatctg
     3481 ctgtttttca aactgattaa tcagcaaaaa tagcttaatc gtcagaataa ccgcaatcag
     3541 ggacgtacag ataaaatcga taacgcctgg ggtgataaat ttgagtttat agttttctat
     3601 aacataatta ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac
     3661 cgcatggaat aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag
     3721 aatcaatgct gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag
     3781 ttcagcgatc attgttttat caggctcctc cagataattg tcgtcatgcc ggaaccccct
     3841 ggcggggcta ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag
     3901 caacattag
//
LOCUS       DAJTOR010000251         3874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTOR010000251 DAJTOR010000000
VERSION     DAJTOR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.255"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(37..594)
                     /gene="ymfL"
                     /locus_tag="OO249_003978"
     CDS             complement(37..594)
                     /gene="ymfL"
                     /locus_tag="OO249_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HCR5638076.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAKLPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDIEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(638..838)
                     /locus_tag="OO249_003979"
     CDS             complement(638..838)
                     /locus_tag="OO249_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5638077.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTQGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            929..1603
                     /locus_tag="OO249_003980"
     CDS             929..1603
                     /locus_tag="OO249_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5638078.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPIGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALHPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            3133..3669
                     /locus_tag="OO249_003981"
     CDS             3133..3669
                     /locus_tag="OO249_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HCR5638079.1"
                     /translation="MSFIQTLTGKKFNYINSTIDDIYIEDIAGALSNICRFAGHLPDF
                     YSVAQHSVLCSQLVAPEFAFEALMHDATEAYCQDIPAQLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPLLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            3660..>3874
                     /locus_tag="OO249_003982"
     CDS             3660..>3874
                     /locus_tag="OO249_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638080.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     LENKVIPVSTELPPANESVLLFDANGEG"
BASE COUNT         1179 a          841 c          811 g         1043 t
ORIGIN      
        1 tcagttgcga gcgacggtag tgtgttgtta aactgttcat gcgttagttt ctccacaacc
       61 agaagcaatc gacgccacga cgcccggagc tgcacactcg cgggcgtcat tactttctga
      121 aatgcaaaaa attttgtaga caagtgctgc atgctcctgc agcttcgaaa ttgagagata
      181 cagctcgtcg ttaattgctg tcttctcatg cggttccact acaccgtctt cgattgctga
      241 acgaatctgt tttgaataac tgccgatctg ttcaatgact tccagcagac gctggttaat
      301 atcggcgttg tccacatcct cgatgtcagg aagagacaca aagacgccat ttgcagactg
      361 cgccacagca tcagcaatga agtgagtgcc accagcacgc tgtaaaacca ttgcccatcc
      421 cagcgggaaa atctgatcgc catctgcacg aaggcggttg aataaagcgt tttctgttac
      481 atcgagccag tcagccgctt cagcgtaacc acccggcaac tttgcgatag tttttctgac
      541 agctttcacg taccactcag gctgtttttc tattttccag tgatgcttac ccacgattag
      601 cctcatcgtt ctgtggttaa aaattgaaag tgttctgcta atctttcgga tagatatccg
      661 gtcttaagtc agatttcgta attgcacctg acgtgcattg ctcaagtttt ttagccagca
      721 caaaactggc ttttttatag ccattgaaaa ccagccgtaa gtagccttgt gttgagccaa
      781 cttttccggc caactcaccc tgctgttctt tggttaaaga gtcccaatac gctttcatac
      841 aatatgtacc tccagtgtac acatcacatg attgaaatga accttcaaga tacttgtacc
      901 ttagcggtac aagggtttta atttcgttat gaaaacaatc catgacatcc ggcggtctaa
      961 cgccagaaaa ctgagagatg gtgttggcgg gaattcttcc tttgccacta tgattgatcg
     1021 cgagccaacc cagaccagca ggtttatggg agatggtgct actaaaaata tcggtgacag
     1081 catggcacga cacattgaaa aatgtttcga cctgcctatc ggatggctcg atcaagaaca
     1141 ccagacaacg aacatcacaa aaaaacctga tgtttcaatc actaacaaac aaataacgtt
     1201 agtccctgtc atatcatggg tacaggccgg agcatggaaa gaagttggct attctgaggt
     1261 tgatttgagc acagcagaaa cttatccctg ccctgtaccc tgtggcgaaa tgacttatat
     1321 cttgcgggtg attggtgatt caatgattga tgagtaccgt ccgggagaca tgatttttgt
     1381 tgatcctgaa gtccctgctt gccacggtga cgacgttatt gcgttgatgc acgatacagg
     1441 cgaaaccacc ttcaagcgat tgatagaaga tggaacacag cgttatctca aagcattaca
     1501 cccaaactgg cctgagccct acattaagat taacggtaat tgctctataa ttggcactgt
     1561 gattttctcg ggaaaaccaa gaagatacaa aataaaggcc taatcaatat ttatgaacct
     1621 gcttcggcag gtttttttat acttgacaat gtaccccatg gacacacaat gtacccccat
     1681 ggacacacaa tgtaccaaaa cgaaacaaca aacaggcagg atgcccacga agtagccgcc
     1741 tggggcatat gaagtccaag atgattcgtt agcaacaaaa cgcccatagg acgcttcgct
     1801 ctttaacaat ttgaatattt tcagcgtcaa tgatctgttt agctccctac aagtaaatgt
     1861 agcgtagcac catgataaat cctgctagtg tttagttaaa aatcgcgcga gagcaagaac
     1921 gacgtgaggg caggcaatac tgatagtgtt attgctatta gtttatcaaa aatatttaga
     1981 cgacccagat gaatattttt aatacatact ccccctaatt taacaggagt aaatgttgat
     2041 gaataacgag acatctatac tcaacaattg tatgatatga acaattatat aacataatgt
     2101 aggaaaaata aaaatggatt ggcatgacct tatatcaaag gtaattatcg gacttgttac
     2161 aggctcaatc gcagcttact taactgcgcg atatgcactc ggccgttatt ataaagaaaa
     2221 atggtgggac aaaagaatag atttgtatac gtaattagtt gactgtgttt atcttctaaa
     2281 aaacacagct gactattggt ataaacatga gttacatcac gcttcacaat tcccaaccta
     2341 ctttagttac aaatcaaaag aagaaaatga aatccttagc aaatctttct ctaatgagct
     2401 tgaaaaactc agtagaatag gtgagctatc ttcattattc ttaacagaag agtgtgggga
     2461 taaaatccgt tcattcattg aaaatgaaga aaatctattt atacaatggg tgtttgatag
     2521 tatagagttg atagatgccc acaagtcaag acttgataaa ttaaaagtat tactagacga
     2581 tattatctct gaagcaaaga gagagcttaa ggtcaataaa aaacataaca gcctaaagaa
     2641 tataaagtgg tccataacta tgccagacca aaaaaatgat gagccaatta attatcacga
     2701 caatcattaa aacactttag aacccgctgg gcgggttcta ttcaaatata ctttgcacat
     2761 cacaaataaa actataacgt ccttcttctt ttcctaatat cttatatcta cttatttata
     2821 agcggcttac agatcttcaa cagataaacc taatgaaggc gacaatgttc tgatgaatag
     2881 ccaccttttt actcaactga aagcgcgttc tgtcccttgt cattaagtgc cagttcgtta
     2941 agtccaaaac ctccggaacg cgctttcaat tgagtgggga aaataactgg cgatggcagc
     3001 cgcccgttgc actaagtgcc tctggatggg gcatttactg aaacgaaacc aaaactttta
     3061 atcgccttat ggcgagggat tcgtgcaacc aaaaatcgtg cggtgcagcg caccaattaa
     3121 ggagaactaa cgatgagttt tatccagact cttaccggta agaagttcaa ttatatcaac
     3181 tccaccattg acgatattta tatcgaggat atcgccggtg cgctttcaaa tatctgccgc
     3241 ttcgcgggac acttgccgga tttttacagc gtggcgcagc attccgttct gtgcagtcaa
     3301 cttgtggcgc cagagttcgc ctttgaagcg ttaatgcatg atgcaacaga agcgtattgt
     3361 caggacatcc ccgcgcaact gaaacgcctt cttcctgact ataaacggat ggaagaaaaa
     3421 atagacgccg taatccgtga gaaatacggg ttacccccgg ttatgagcac gcccgtgaaa
     3481 tatgccgatc tcatcatgct ggcaaccgaa cgccgcgatc ttgggcttga tgatggctct
     3541 ttctggcctc tactagaagg tatcccagca acagagatgt tcaaagttat tccactgtcg
     3601 ccaggccatg cctacgggat gtttatggaa cgttttaacg aattatcgga gttacgcaaa
     3661 tgcgcatgaa tgttttcgaa atggaagggt ttcttcgcgg gaaatgtgta ccgcgagatc
     3721 tgaaagtgaa tgaaacggat gctgaatacc tggtgcgtaa attcgatgcg cttgaagcta
     3781 aatgtgcggc actggaaaac aaagtaatac cagtgtcaac tgaactgcca ccagcaaatg
     3841 aaagtgtttt gttattcgat gctaacggag aagg
//
LOCUS       DAJTOR010000252         3837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTOR010000252 DAJTOR010000000
VERSION     DAJTOR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.256"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(521..712)
                     /locus_tag="OO249_003983"
     CDS             complement(521..712)
                     /locus_tag="OO249_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5638081.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            780..1103
                     /locus_tag="OO249_003984"
     CDS             780..1103
                     /locus_tag="OO249_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5638082.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            1066..1809
                     /locus_tag="OO249_003985"
                     /pseudo
     CDS             1066..1809
                     /locus_tag="OO249_003985"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1781..2341
                     /locus_tag="OO249_003986"
     CDS             1781..2341
                     /locus_tag="OO249_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5638083.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENILNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            2348..2581
                     /locus_tag="OO249_003987"
     CDS             2348..2581
                     /locus_tag="OO249_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5638084.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLVEQVDHLSRLFITNSR"
     gene            complement(3051..3518)
                     /locus_tag="OO249_003988"
     CDS             complement(3051..3518)
                     /locus_tag="OO249_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638085.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     AFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          913 a          877 c         1145 g          902 t
ORIGIN      
        1 accaggggga aacgcctggt atctttaagt cctgtcgggt ttcgccacct ctgacttgag
       61 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc caccggcgcg
      121 gcctttttcg ggctcttgtt ttttgctcac atgtttcttt tggctttatc ccctgattct
      181 gtggataact gtgttaccgt ctttgagtgc gctgataccg ctcgccgcag ccgaacgacc
      241 gagcgtagcg agtcagtgag cgaggaagcg gaagagcgcc ggaagctaca ttttctcctt
      301 acgcatctgt gcggcatttc acacccggca tggtgcactt ttcatacaat ccgcactgat
      361 gccgcataag taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
      421 cccgacaccc gctaaaacct gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      481 ctcacaggcc agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccatcat
      541 ccccgaagcg tgcgaggcag ctgcggtaaa gctcgtcagc gtggtcgtga agcgcttcgc
      601 agatgtcagc ctggtcgtcg gcgtccagtt cattcagttt ttccagcagg gtcagcgtct
      661 ggcttctgat gaagcgggcc atgttaaggg cggttttttc ctgtttagtc acagttctgt
      721 ttctccatgt gtggggagtc tgtcggaggt ttttaatgat accggtgaaa cgcgaaagga
      781 tgctcacgat acgggtcacg gatgaggaac atgcgcggct gctggcacgt tgtgagggaa
      841 aacaacttgc gtcatggatg cgtaaggtct gcctaggggc accgccatcg aaaacgtcag
      901 gtcttccgac actggcaccg ccgctgttgc gccagttcgc ttcggtcggc aataacctga
      961 atcagatagc ccggaagata aacagcggac agtggtcggg gcatgaccgg gttcatgtgg
     1021 tggctgcact gatggccatc ggacgtgaac tgagtgagct gagggatgag gtccgtaaac
     1081 agggagagcg ggatgatagt taagtttcat gccaggggaa aaggcggtgg cagtggtccg
     1141 gttgattacc tgctgggccg ggagcgtaac cgggaagggg cgagagttct gcggggtgct
     1201 cccgaagagg tgcgggaact catcgatgcc acgccgtttg cgaaaaaata cacctcgggg
     1261 gttctgtctt ttgcagagca gaccctgcca ccgggagagc gtgaaagggt gatggagagc
     1321 tttgagcggg tactgatgcc cggtcttgaa aaaaatcagt acagcatcct gtgggtggag
     1381 caccaggaca aggggcgtct tgagctgaat tttgtcatcc cgaatatgga gctggcgagc
     1441 ggaaaacggc tccagcccta ttacgaccga gcggaccgtc cgcgaatcaa tgcctggcag
     1501 acactggtga atcaccatta cgggctgcac gacccgaacg caccggagaa caggcggaca
     1561 cttgtcaccc cgaataatct gccgaaagcg aaacaggagg ccgcagaagc gattacgagg
     1621 ggactggagg ctctttacca tgccggagcg ctaaaaacgc gtcaggatgt cactgaggcg
     1681 cttacagcgg caggttttga ggttgtgagg acaaccagga gcagtatcag tatcgccgac
     1741 ccggagggcg ggcgtaatct caggctgaaa ggggcgctgt atgagcaatc ttttacaaat
     1801 ggggacagac ttcgagaaga aactgaaaga gcgtgccgtc tctacagaga acatactgaa
     1861 cagcgagttc aggaagctcg aagcgtctgt agacagggaa ctgaacttaa acgggcagaa
     1921 aatcagagac gctatcagcg cacacacagc agcggtgaaa gaacaactgg agacgctgaa
     1981 cagtgccgtg aacgcgcagt tcagcgccac agagaacgaa ctggcccggc ggcaggaaga
     2041 gctgctctgg aagctggtca aagggcggat actgtacccg tcgctgacgg cgctttgcgt
     2101 cacgggcggg atttttctgg cgtcatgggg gctgattcag tggcaggaaa gccggattgc
     2161 ggcgaacatc ctggctatca gggaccagga agagacgctg gcgaaactcc gggaaaaaac
     2221 atggggtgtg acgtatcacg agggcaggaa cgggaaattt ctggtattgc cgtccggggt
     2281 gaagggggaa aacaactgga cggtggagaa gaagaacgca gtaagactgg tcagggagta
     2341 agtgagtatg acagagctgg aaatgcactt actgaacgcc ttagagcaat tgcagcagga
     2401 ctatacgcag cggctgaaag actgggagag cgccttcacg gatttgcagc atatgttcga
     2461 gctgacacga caggagaacg ggcaactgag ggaacagtgc gacgacttga gtcagcaggt
     2521 gcagagatta gtcgagcagg tggaccactt gagtcggtta tttatcacga acagcagata
     2581 agggatgaac aggaaaggca ggcacgcctg gaacgggagc gtgcgcggtc ctggcatgaa
     2641 gagcaggaac gggaagcacg ggtatggcgt ggcccgtcaa tagggatatg acggtgagtt
     2701 gttccgggag gttgccagat ggtaccggaa cgcgttaaaa acgcatccgg gcgcgattct
     2761 ggcgtgtcgt ttttgttgct gtgttttgcc tctttgccag atcgcgctca ttcctcagcc
     2821 tcatcccgcg atgtctgcgt atgtcatcaa ctgaaaagat ggaaaaaaaa cagtgtcatg
     2881 aggtttacgc aggcaaaacc agcgttattc acatggctga atagctatgc atacggttaa
     2941 aatttatcag gtgcgatcgc ggcagctttt cgggtggttt gttgctattt ttacctgtca
     3001 gcagccgtga tcgcgctgaa cgcatttcag cggtgcgtac aattaagata ttacgttaaa
     3061 ttgtgaggga atggtcccca tatactgact ttatcatagg gatagggttg gtagtgggtc
     3121 gattgggttt cggcattgtt tccgttaatg ccaattgcat ttttgtattt gatgcaacag
     3181 aaggcgtgag attctcctgg gcctggaaag gctaagtgtt tagaattgat ggcaaaatat
     3241 cttccgtccg atttttgaag tagttgttgt ttggctgtct cccaaagggt gctgttacaa
     3301 acttcttgaa tttgtagttt ttgcagggct cttattactg cgttgtcatg ttccaattct
     3361 ttaggggagt gtattattct ctcttttaat aagctgttta ccacagggtt tagttgttgt
     3421 ctaaagcacg cagataatgc cattgctaca caattttgtt cccgaggaag catgtttggc
     3481 ggggtatgcc gtgtttgatg tgatatgtct attctcatag aaggtctctg gcttatagca
     3541 tcaacaaata cggctataaa acaaaatttt catagagtga ttcaagatct tcttgagatc
     3601 cttttttgcg gcgcgtaatc tgctgcctgc aaacaaaaaa accaccgcta ccaacggtgg
     3661 tttgtttgcc ggaatctaga gctaccaact ctttcttccg aaggtaactg gcttggtgga
     3721 gcacagatac caaatactgt ccttctagtg tagccgcagt taggccacca cttcaagaac
     3781 tcttaatatc tcaatccacc ttgtctagtt accaatggct gctgccagtg gcgttaa
//
LOCUS       DAJTOR010000253         3784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTOR010000253 DAJTOR010000000
VERSION     DAJTOR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.257"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            115..618
                     /gene="yeaK"
                     /locus_tag="OO249_003989"
     CDS             115..618
                     /gene="yeaK"
                     /locus_tag="OO249_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5638086.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
     gene            complement(619..723)
                     /locus_tag="OO249_003990"
     CDS             complement(619..723)
                     /locus_tag="OO249_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638087.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            892..1338
                     /locus_tag="OO249_003991"
     CDS             892..1338
                     /locus_tag="OO249_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5638088.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1295..2116)
                     /gene="nimR"
                     /locus_tag="OO249_003992"
     CDS             complement(1295..2116)
                     /gene="nimR"
                     /locus_tag="OO249_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR5638089.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2213..3409
                     /gene="nimT"
                     /locus_tag="OO249_003993"
     CDS             2213..3409
                     /gene="nimT"
                     /locus_tag="OO249_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR5638090.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     RSYPVL"
     gene            3449..>3784
                     /locus_tag="OO249_003994"
     CDS             3449..>3784
                     /locus_tag="OO249_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5638091.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFTTFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWL"
BASE COUNT          872 a          961 c          953 g          998 t
ORIGIN      
        1 ttacatgatg cgtataaagc ttccgatgag cgtttatatg tcaataagca gaacaaaaac
       61 agccgttcat gataaccttc tgtggttgtt tgcttgtaat ctcaggagcg tgaaatgact
      121 gaaatggcta aaggaagcgt gacgcatcag cggttaatcg ctttattatc acaagaaggt
      181 gctaacttcc gcgtcgttac ccatgaagcg gtaggtaaat gtgaagcggt gtcagaaatt
      241 cgtggcaccg ctttaggcca gggagctaag gcactggtat gtaaagtcaa aggcaatggc
      301 gtaaatcaac atgttctggc gatcctcgct gccgatcaac aggccgatct gagccaactt
      361 gccagccata taggcggatt acgcgcttcg ttagccagcc ctgctgaagt cgatgaactc
      421 acaggctgtg tcttcggcgc gatcccccct ttcagcttcc atccaaaact caaactggtt
      481 gccgaccctc tactcttcga acgatttgat gaaatcgcct tcaatgcagg gatgctggat
      541 aaatccgtta ttttgaaaac cacggattat ttgcgcattg cgcaaccaga actggtcaat
      601 ttccgccgca ctgcgtaact agcctgtccg ttcaataagc agaacggaca aaaccagcac
      661 aacagcaatg gcaaaaaacg atgacgtgat aatcagtgtc tcgacgaaca tttgatcgtt
      721 catagcatgc ccccgggaga tagctgtttt cacgttattg ttagcgtgca caaatggcag
      781 tttgatgaca gttcgcctga tttttatgca aaaaacgtaa agattttcta cttccttcct
      841 gcagcaagcg taaagtaagc aggcttatta ttttttggca aggaaaccac gatgtttgat
      901 gtcactttgc tgatcctgct cgggttagct gcgctgggct ttatcagtca taacaccact
      961 gtcgccgttt caattctggt gttgatcatt gtccgcgtca caccgttaag cacctttttc
     1021 ccgtggattg aaaaacaggg gctaagtatc gggataatca ttctgacgat tggcgttatg
     1081 gcccccatcg ccagcgggac gctaccgccc tcaaccttga ttcactcgtt tctgaactgg
     1141 aaatcgctgg tggcaattgc agtaggcgtg attgtctcct ggctgggtgg gcgcggcgtg
     1201 accttaatgg gcagccagcc gcaactggtc gccgggctgc tggtaggcac tgttttaggc
     1261 gtggcgctgt ttcgcggcgt accggtcggc ccgcttattg ccgcgggtct ggtttcgctg
     1321 attgtgggga aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt
     1381 tcttaaacat agtaataaag gccgttgttg aatcataccc cagcgtatga gcaactttct
     1441 gtacagtatc gcctttcact aatccctgaa gtgccataat cagctgtaac tgctgacgcc
     1501 actggcgaaa actcaacccc gtctctttta cgatcagtcg cgcaagattg cgttcactca
     1561 tcgcaaaaaa gccagcccat tgccctaatg acccccactc gacgggccct ttcgccatca
     1621 tctccaccat cgtgcggatt ttaggatgag aagaaacggg caggtgcagc tgttgttgtg
     1681 gctgctgagg gagttcatca aacaggacct gtattaatct gcgggtcatc ggctcagcac
     1741 gctgagtcgt cgtcctgttt gccagggtta aaatcaattc ccggcataag ggcgatattt
     1801 tcaatgtgca acatgttgtc ggcattgtca ctgcgctggg ttcgataaac agaaaacaga
     1861 gttcagcgtt agctgtcact tgattgctat gttccacccc gcccggtatc caaaccgcat
     1921 attgcggcgg caccatccac aaagcatttt ccaccgtaca ggtaattgca ccatgtaatg
     1981 ctagtatcag ctgtcctttg cggtgctgat gtacaggact ggttaactca tccgttccgg
     2041 cgtgaataca aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag ccattgagat
     2101 tcaacctgtg catcattttg tccgaattta gcgataattt gtcattttag cttgattcaa
     2161 cataacaata aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc atatgacctg
     2221 ttcaacttca ttaagcggca aaaacaggat tgtccttatc gctggcattc tgatgattgc
     2281 cacaacatta cgcgtcacct ttaccggcgc agcaccgtta ctggatacga ttcgttccgc
     2341 ttactcgctg acgacagcgc aaaccggctt attgaccacc ctgccattat tggcctttgc
     2401 gctaatctca cctttggccg ccccggtagc gcgacgtttt ggtatggaac gtagcctgtt
     2461 tgccgcgtta cttttgatct gtgctggtat cgcaattcgc tctctccctt cgccttactt
     2521 attatttggc ggtacagcgg tcattggcgg tgggattgca ttaggcaatg tcttactgcc
     2581 aggattaatt aaacgcgatt tccctcattc cgtcgccaga cttaccggcg catattccct
     2641 gacaatggga gctgcggcgg cacttggatc ggctatggta gtgccgctgg ctttgaacgg
     2701 ttttggctgg caaggcgcgt tgctcatgct gatgtgtttt cctctgctgg ctcttttttt
     2761 atggctgcca cagtggcgaa gccaacaaca tgcaaatttg agtacctcgc gcgccttaca
     2821 tactcggggt atctggcgtt cgccgcttgc ctggcaggtc acattgtttc ttgggagcaa
     2881 ctcactggtc tattacgtga ttattggctg gcttccggcg atcctcatca gtcacggcta
     2941 tagcgaagca caggcgggtt cactgcatgg tttgctgcaa ctagccacag cagctcccgg
     3001 tttgctgatc cctcttttct tacatcatgt gaaagaccag cgtggtattg cagcgttcgt
     3061 tgccttgatg tgcgcagtgg gcgctgttgg gctctgcttt atgccagcgc acgcgatcac
     3121 ctggactctg cttttcggtt ttggttccgg cgcaacaatg atactggggt tgacgttcat
     3181 tggtcttcgg gcaagttctg cgcatcaggc ggcggcactc tcggggatgg cacaatccgt
     3241 cgggtatttg ctggcagcct gtgggccgcc gctaatgggt aaaatacacg atgctaacgg
     3301 taactggtcc gtaccactga tgggtgttgc aatactttca ctactgatgg cgattttcgg
     3361 actttgcgcc gggagagaca aagaaattcg ctcatatccg gtgctatagt gacgtgacaa
     3421 atcatgcgtg aaagggagaa caaacacgat gaatattcag tgcaaacgcg tttatgatcc
     3481 ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa
     3541 aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa cggaactgcg
     3601 caaagccttt cacggcgaag tcgtcgattt tacaaccttt cgcgagcaat atcttgcaga
     3661 actggcgcaa cacgagcaag aaggaaagcg gctggcggac atcgccaaaa aacagccgct
     3721 gaccctgctc tacgcagcaa aaaacaccac gcagaaccat gcgctggtgc tggccgactg
     3781 gcta
//
LOCUS       DAJTOR010000254         3724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTOR010000254 DAJTOR010000000
VERSION     DAJTOR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.258"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            37..264
                     /locus_tag="OO249_003995"
     CDS             37..264
                     /locus_tag="OO249_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5638092.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(265..639)
                     /locus_tag="OO249_003996"
     CDS             complement(265..639)
                     /locus_tag="OO249_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5638093.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="OO249_003997"
                     /pseudo
     CDS             complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="OO249_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2118..3038)
                     /gene="lpxL"
                     /locus_tag="OO249_003998"
     CDS             complement(2118..3038)
                     /gene="lpxL"
                     /locus_tag="OO249_003998"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5638094.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3263..3724
                     /locus_tag="OO249_003999"
                     /pseudo
     CDS             3263..3724
                     /locus_tag="OO249_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
BASE COUNT          915 a          952 c          945 g          912 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc attattagtc gcactatacc ggggcagggg
      121 catggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ctggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 attcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg ctgcgcgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcgtatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg cccggcgatc tggttgctcg agttgtaaac caacaatgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgct gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga ccggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggtggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ccaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctga agacaatacc ggaccatatt cagggcggag
     1801 tgaccggtaa cgccaagctg ctcaacgtag aggggtaaga agggcattac cagactgaag
     1861 gcggcaccgg taagaaaaca gcctagccag gcgacgatca ggtttcgttt ccagtttata
     1921 ggggtgtcat tttcacaggg tgacatagca atccgctgtt ggtgcgccag gcgcggtgaa
     1981 cataagaaga aaagataagc acactaatta tgcgcccgac ttccaggggg cgcaatccag
     2041 agagctttta tcgctaaatc agggggattt gctgtggtaa tgccggatgc cattctgaag
     2101 catccggcag gggagattta atagcgtgaa ggaacgcctt ccgggcgtgt tttaaagcga
     2161 cggtgtaacc acatatactg ctctggtgcc atcatgatgc atttttcgac cactttgttc
     2221 atccacgcgg cggtagtttc ggcatcatcc agtggcggag aacactctgg cggcagcata
     2281 atcaattgat accctttgcc atctggctta cggcgtggaa cgaagggcac cagacatgcg
     2341 ccggacatcc gtgccagcat ccaggttccg gtcgtggtcg cagcctgctc aacggcaaat
     2401 aacgggacga aaacgcttga gcgcgggccg taatcatgat ccggtgcgta ccagaccact
     2461 tcgccttttt tcagggcttt aatcatgcct tttaaatctt tgcggtcgag catcgattta
     2521 tttgagcgca aacggcccca ggtttgtagc cagtcaatca gtggattatc gttcgggcgg
     2581 taaacgccaa tccccggttc ctgcatacca aactgccgcg cacccagctc cagtgtcaga
     2641 aaatggatgc caactaacag gatgccgcgt ttttgcgcct gcacgtcacg aatgtgttcc
     2701 atgccgatca cttccgtcca gcgcgcgatt cggcggtccg gccagaacca cgccatgccg
     2761 gtttccatca ggcccatgcc aacggattcg aaattcttca ccaccatttt acggcgttct
     2821 tgttcgctca tttccgggaa gcacagttcc aggttgcgat gcacaatttt tgcgcgtcgt
     2881 ttcataaaac gtaacgccag ttttcctaat ccacaaccga ggcggtagat aaccgggtag
     2941 ggcaattgca cgactaacca aagtacgcca atacccaacc aggttaacca ataacgcgga
     3001 tgaagcagtg cggtggagaa cttgggtaga ttcgtcatat caatcctgtt tttcaaccta
     3061 ttcgggcaat tgtatgtatt gtcgcatttt ttcgcccgca accaaaattt gtggctgaag
     3121 actgggcgaa attgccgcgc ttgtaaataa caaataattt ttaatgcgca aatgtagcgt
     3181 aaaatgtgtg gatgttaatt atcgataatt gctatatcat gccgcggatt tttactttcc
     3241 catctcgcag gaaccgtaca ccatgccagt gttacacaac cgcatttcca acgacgcgct
     3301 aaaagccaaa atgttggctg agagcgaacc gcgaaccacc atttcgtttt acaagtattt
     3361 ccacatcgcc gatcctaagg cgacccgtga cgctttatat cagctgttta ccgcgctgaa
     3421 tgtttttggg cgagtgtatc tggcgcatga gggcattaac gcgcaaatca gcgtacctgc
     3481 gagcaatgtt gaaacatttc gcgcgcagct ctgccttcga cccggcttta gagggtttac
     3541 gcctgaatat cgcgttggat gatgacggga aatccttctg ggtactgcgc atgaaggtac
     3601 gtgatcgcat tgttgccgac ggtattgacg atcctcactt tgatgccagc aatgttggtg
     3661 agtatctgca agcggcggaa gtgaacgcca tgcttgacga tcccgatgca ctatttatcg
     3721 acat
//
LOCUS       DAJTOR010000255         3694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTOR010000255 DAJTOR010000000
VERSION     DAJTOR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.259"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(152..571)
                     /locus_tag="OO249_004000"
     CDS             complement(152..571)
                     /locus_tag="OO249_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5638095.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            651..905
                     /locus_tag="OO249_004001"
     CDS             651..905
                     /locus_tag="OO249_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5638096.1"
                     /translation="MNQKTLEDVIKTVRVAVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            902..1327
                     /locus_tag="OO249_004002"
     CDS             902..1327
                     /locus_tag="OO249_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5638097.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDALKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            1399..2469
                     /locus_tag="OO249_004003"
     CDS             1399..2469
                     /locus_tag="OO249_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR5638098.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRYVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQAMTASPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            2510..2932
                     /locus_tag="OO249_004004"
     CDS             2510..2932
                     /locus_tag="OO249_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5638099.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRILSFYQGNFQEVMA"
     gene            2929..3234
                     /locus_tag="OO249_004005"
     CDS             2929..3234
                     /locus_tag="OO249_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCR5638100.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQETASVQD"
     gene            3221..3676
                     /locus_tag="OO249_004006"
     CDS             3221..3676
                     /locus_tag="OO249_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5638101.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSAPDVLALLKELEVKDKRIAELTDALTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
BASE COUNT         1016 a          780 c         1005 g          893 t
ORIGIN      
        1 gcattctgtg gggcggggtg ggtggcaggt atataatgta ctttgcgttc attgttgtaa
       61 agtgcttatg gtacattttg tgtgtaaaaa aatgagatgg gataaagtga agcacaaacc
      121 cggaggaagg cgctaccgga tttatgctgg tttaagaggc tttttgtttt ttctttcgtg
      181 ctaactcttc gtaaattgca ttgtacttct gttttttctc ttcaagagtt tttaaaagtt
      241 catctgtctc actgtcaggg agctcgtcca gaaggtcaat gatgattttt tgtcttggat
      301 ttaactcctg atagaaacgt acctgtccac tttcttctgt atcctctccc aaaagatagg
      361 ttggtgttgt tcctattagt gttgctaatt cccttaattt ctcccggcga ggaattgttt
      421 cgccattaaa ccatttgcta accgcttttg gtgttaattt cattcgacgg gcaatttctg
      481 cctgccttcc atgttgttca taaccagcgt tttcacaggc tagcgcaagc ctactggcga
      541 actctttacg cgctttatct tcatgaacca taagttcaat gatattcgct cttgaatgta
      601 ctgtcagttc tgttatagca tgtactcaaa gttcacattg tgagggtgat atgaaccaga
      661 aaacacttga agatgtaatc aaaactgttc gcgttgctgt tgtggccgac gtttgtggtg
      721 tcagccaaag agcaatctat aaatggatgg ataacggaaa attgcctcgc acagaatata
      781 ccggcgaaac aaattacgct gaaaaaatcg ctcttgcatc aaacggatta ttttctgccg
      841 atgcaatttt aactattggc aggaataaaa ctactacgaa aaagctgatg ggagttgatt
      901 catgaaaatc aagcatgaac acatccgcat ggcgatgaat gcctgggcgc atccggacgg
      961 cgaaaaagta ccggctgcga aaattaccaa agcgtatttc gagctgggaa tgacgttccc
     1021 ggaactgtat gacgacagcc atccggaagc cctggctcgc aatactcaga aaattttccg
     1081 ctgggtggag aaagacaccc ctgatgcgct taaaaaaatt caggcgttgt taccagctat
     1141 cgaaaaagca atgccacctc tgctggtggc ccgaatgcgc agccatagct cagcctattt
     1201 tcgggaacta gtggagacgc gggaacgact ggtgagagac gctgatgatt ttgtcgcagt
     1261 ggcgatcgct ggtttcaacc agatgaatcg tggtggccct gcaggaaata ttgtggctgt
     1321 gcattgactc gcaatattca taccggatca cttccggcaa tttgtgagta aaaagattcg
     1381 gtatcaaaag aggtgagtat ggctaacgcc tggctcagat tatggcatga catgccaaat
     1441 gaccctaagt ggcgaacaat tgccagggtg tcagggcagc caattgcaac agtgatggca
     1501 gtgtatatcc acctcctggt gagcgcgtca cgaaatgtca cgcgaggtca cattgatgtc
     1561 acgacagaag atttggcaag tgcgctcgac gtgacagaag aggtaattga ttcaattttg
     1621 cagacgatgc aggggcgggt acttgatggt gatttaatca ctggatggga aaaacgccag
     1681 gtgctgaaag aggacaacgg caatatttcg caaaccgcaa aatctcctgc agagcgcaag
     1741 agggcgcagc gagagaggga aagaaagcgg gaacaaaatg gcgattgtca cggcgcgtca
     1801 cgatatgtca cgcacatgtc acgacgagtc acgacagata aagatacaga taaagataca
     1861 gatcaagaag atcaaaacac tatggtccat ggcgtaaaaa acgccacgaa ccaggcaggg
     1921 gatgttcaga ccgtcaatcc tggtcagcca gcaggcacga caccggaagc cgattcagcg
     1981 tatgcgctga aagccgattc gggcgctgtg cagcaggcga tgaccgcaag tccggagcaa
     2041 tcacaccaac tgcagcagcc tgaagccgat tccgccattc agcgggaagc cgatcgggta
     2101 gtcccggaaa acaccgggca gtctgtggga cgagtggatt atccggatgt gttcgaacag
     2161 gtctggcggg agtacccgtt gcgtgccggg gcaaacccga agaaatccgc tttcagtgcc
     2221 tggaaggcca gattacgcga gggggtgcca ccagaggcca tgctggatgg cgtgaggcgt
     2281 tacgcaagat acctggcggc taccgggaaa acgggaacgg aatttgttca gcgagcgacg
     2341 acgttttttg gaccggaccg gaattttgag aacccctggt tgctcccggt aagcggcacg
     2401 aacaaccagc gttgtgtgaa tcatatttct gaaccggata acgaaattcc gccgggcttc
     2461 agggggtaag tgttaatttc tggtcatgag gtaattttca ggagggcttg tggcaaaagt
     2521 ttttacacaa gaagagcggg aaaaaattaa agggcaggtt cttgaactcg tacgccagag
     2581 tgggcgcgag acgttacgac aactggaagc taaaactggg gcaacaagat atctgatgag
     2641 cgttctggcc agagagctgg ttgccagtgg cgatgtatac aactctggtt acgggttatt
     2701 cccgtctgaa caggcgcgta aggactggca aaatgcccgt aaaaagctct caagggcaaa
     2761 gctgaagaaa ccatctgcgg ttgatccgga ccttatctgg tcattacctg acggagaaat
     2821 acgtcgctac gacagtcgcc taaacataat ctgtcgcgag tgccggaaga gcgaagttat
     2881 gcagcgcata ttgtcgtttt atcagggtaa ttttcaggag gtgatggcgt gagggtgaga
     2941 gtctatatcg ccggtccaat gaccgggtat aaaaatttca accgtgaggc gtttcacaat
     3001 gcggaagagg aactgaaacg ggaagggcat accgtcttaa acccggcagt acttccggac
     3061 gggctgacac agccgcagta catggatatc tgcatggcga tgattcgttg tgtggatgcg
     3121 atttacatgc tgaaaggctg gcagcggtca gcaggcgcta aggcagaact ggcgctggcg
     3181 gagaaactgg ggcatgcggt gatttttcag gagacagcca gtgtgcaaga ctaattacca
     3241 ggcattacgt gaacgttatt caccaattca ggtgccggaa tgtcctgttt gtggcgatga
     3301 aatgtcgata cagcgcatat tttccagaac gcatattgtg tatgcctgca caggtgaggg
     3361 ggatgatgga tattttaaaa ctggtcgaac ttttgcggat gagcattacc tgaaatcgcg
     3421 cgtaaccgtc gttgatgtta gcgctcctga cgtactggct ttactgaaag agctggaagt
     3481 taaagacaaa cgcattgcag aattgacaga tgcgcttaca caaatgatta atgcgcacaa
     3541 aaccacaatt cgttttggtc atgaacgcat aactgaatgt ggtggtgatt gcgactcgcc
     3601 ggaaaagatg atttcagaaa atccggatat cagaatggca gaggctgttt tgagagcagg
     3661 aataaaaact gaataattaa atttagcaca gcaa
//
LOCUS       DAJTOR010000256         3684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTOR010000256 DAJTOR010000000
VERSION     DAJTOR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.260"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..441
                     /locus_tag="OO249_004007"
     CDS             <1..441
                     /locus_tag="OO249_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5638102.1"
                     /translation="RFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGV
                     RISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVI
                     AEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            449..1777
                     /locus_tag="OO249_004008"
     CDS             449..1777
                     /locus_tag="OO249_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5638103.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGSYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1824..3149)
                     /gene="rimO"
                     /locus_tag="OO249_004009"
     CDS             complement(1824..3149)
                     /gene="rimO"
                     /locus_tag="OO249_004009"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5638104.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            3362..3684
                     /gene="bssR"
                     /locus_tag="OO249_004010"
                     /pseudo
     CDS             3362..3684
                     /gene="bssR"
                     /locus_tag="OO249_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
BASE COUNT          960 a          819 c          911 g          994 t
ORIGIN      
        1 cgttttacca ccatcatgaa agaaaatggc ctgaaggcga acagcctctg tgttgagatc
       61 actgaaaccg tgatcgagcg aattaatgaa catttttatc tcaatattga acaactgcgt
      121 aaacaagggg tacggatatc gattgatgac tttggcaccg gtttgtcaaa cctgaaacgt
      181 ttttatgaaa ttaatccaga cagcataaag gtggactcgc aatttaccgg cgatattttc
      241 ggtactgcgg gaaaaattgt gcgcattatt ttcgatctgg cacgctataa ccggatcccg
      301 gtgattgcgg aaggcgtaga gagcgaagac gttgcacgcg aattaattaa attaggatgt
      361 gttcaggctc aggggtatct gtaccagaaa cctatgccat tctccgcctg ggataaaagt
      421 ggaaaattag taaaagagta gtttacgtat gtccagaatc aataagttcg tacttacagt
      481 cagtctgctg atttttatca tgatttcagc agttgcctgc gggatctaca ctcaaatggt
      541 aaaggaacgg gtgtatagcc tgaaacagtc cgttattgat actgcttttg cggtggcaaa
      601 tattgctgaa tatcggcgta gcgtggcaat tgatcttatc aacacgctaa atcccacgga
      661 ggaacagctg ttggttggtt tgcgcacagc ttacgccgac tcggtttccc cctcttattt
      721 gtacgatgtc ggtccttatc tgatttccag tgacgaatgt attcaggtaa aggagttcga
      781 gaaaaattat tgtgcagata ttatgcaggt tgtgaagtat cgacatgtca aaaatacagg
      841 gtttatctct tttgacggta aaaccttcgt ctattacctc tatccggtaa ctcacaatcg
      901 tagtctgata tttttgcttg gtctggagcg tttttcttta ctgtcaaaat cgctggcgat
      961 ggacagcgag aacctgatgt tctctctatt taagaacggt aaaccggtga ccggtgatga
     1021 atataatgct aaaaacgcca tcttcaccgt ttcggaagcg atggagcact tcgcctattt
     1081 gccgaccgga ttgtacgtat ttgcgtataa aaaagatgtt tatttgcggg tctgtacatt
     1141 gattattttc tttgccgcat tggtggctgt gatatcgggt gccagttgcc tctatctggt
     1201 acgcagagtg attaatcgtg gtattgtgga gaaagaagcc atcattaata accattttga
     1261 acgcgtactg gatggcgggc ttttcttttc ggctgccgat gtcaaaaaac tctacagtat
     1321 gtataactcg gcgttcctgg acgacctgac caaagcaatg ggcagaaaat cctttgacga
     1381 agatttaaaa gcgctgccgg aaaaaggcag ttatttgtgc ctgtttgacg tcgataaatt
     1441 caaaaacatt aacgacacct tcggtcattt gctgggcgat gaagtgttga tgaaagtggt
     1501 gaaaatcctt aaatcacaga tcccggtaga taaaggtaaa atctaccgct tcggcggtga
     1561 cgaatttgcg gtgatttata cgggtggaac gctggaagag ttgctatcga ttctaaaaga
     1621 aatcgttcat ttccaggtgg gaagcattaa tttaagtacc agtatcggtg tagcgcattc
     1681 aaatgaatgt accaccgtcg aacgcttgaa aatgctggcg gatgagcggc tgtataagag
     1741 taagaaaaac ggcagggctc agattagctg gcagtaatca ttattcgcag gccggacaaa
     1801 tgattttgcc cggcctgaat taattaaacc cggctacccc acaaatcgta ctcatcggcg
     1861 tgctcgactt tcacacgcag gatatcaccc ggcttaacgt tggtttcacc attgagataa
     1921 accgcgccgt cgatttccgg tgcatctgcc atgctgcgac caatcgcgcc ttcttcgtcc
     1981 acttcgtcga taatcaccag aatttcacgg cccactttct cttgcaggcg ctcggcggaa
     2041 atctgctgct gcagctgcat gaaacggttc cagcgttctt ctttaacttc ttccggaacc
     2101 tggtcaggca gggcattggc gtctgcacct tcaaccgggc tgtatttaaa gcagccaacg
     2161 cgatccagac gcgcttcttt caggaagtcg agtagcatct ggaaatcttc ttctgtctcg
     2221 ccagggaagc cgacaataaa ggttgagcgt agggtcagtt ccgggcagat ttcgcgccac
     2281 tgtttgatgc gcgccagttg gcgatctaca gaacccggac gcttcatcag tttgagaatg
     2341 cgcgggctgg cgtgctgcaa cggaatgtcc agatacggca ggattttgcc ttctgccatc
     2401 agtgggatga cgtcgtccac atgcggataa gggtaaacgt agtgcagacg tgtccagatc
     2461 cccagtttcg ataactgttc gcacaggctg accatgctgg tttttaccgg ctcgccgttg
     2521 tggaagccag tacgatgttt aacatcaacg ccataggcgg aagtatcctg cgagatcacc
     2581 agaatctctt taacgcccgc atctaccaga cgtttcgctt cacttaacac ttcgccaatc
     2641 ggacggctca ccaggtcgcc gcgcatagac ggaataatgc agaaggtgca gcggtgatta
     2701 cagccttccg aaattttcag ataggcataa tgacgcggcg tcagtttcac accttgttct
     2761 ggcaccaggc tcaggaatgg gttgtgtttc ggttttggca cgtagtgatg aacgtgctcc
     2821 agaacctgct catagctatg aggcccggtg atttccagca ctttcgggtg gacttcgcgg
     2881 atctgatctt cttttgcccc cagacaaccg gtcacaatta ccttgccgtt ttcattcaac
     2941 gcttcaccaa tggcttccag tgattcttgt accgcgctgt caataaagcc gcaggtgttg
     3001 acgatcacca tgtccgcatc gtcatagctc ggtaccacgt cataaccttc agtgcggagt
     3061 tcggtgagaa tacgctctga atcgacaagg tttttcggac agccaaggga aacaaagccg
     3121 attttcggct ggggagttac tttgctcata gcttaaaaaa tattcagtta caggaaaggt
     3181 cagggcaggg attctacaga gttctggata aaatttgtat cgcaatctca ttcgctggcg
     3241 gaggcgaagg aaatgtaaat tttgttaatt cggcgtgaag aattgatcct ggacagcatt
     3301 ttgctcaaaa aatagccata ctatttaatt gcaacaaggc tggaaagagg aggatcgaag
     3361 tatgttcgtt gacagacagc gaatcgatct gctgaaccgg ttgatcgacg cacgcgttga
     3421 cctcgctgca tacgtgcaac tgaggaaggc aaaaggatac atgtccgtca gcgaaagcaa
     3481 tcatctacga gataactttt ttaaaactga atcgcgaact gcacgataaa tcgctgcggt
     3541 tgaatcttca tctggatcag gaagagtgga gtgctcttca tcatgctgaa gaagcattag
     3601 cgacagccgc agtatgtttg atgagtgggc accatgattg cccgactgtt attaccgtca
     3661 acgccgataa gcttgaaaat tgtc
//
LOCUS       DAJTOR010000257         3651 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTOR010000257 DAJTOR010000000
VERSION     DAJTOR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.261"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            232..543
                     /locus_tag="OO249_004011"
     CDS             232..543
                     /locus_tag="OO249_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5638105.1"
                     /translation="MFNVITHPAALDELLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            543..830
                     /locus_tag="OO249_004012"
     CDS             543..830
                     /locus_tag="OO249_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5638106.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            complement(1367..2554)
                     /locus_tag="OO249_004013"
     CDS             complement(1367..2554)
                     /locus_tag="OO249_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5638107.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(2554..2919)
                     /locus_tag="OO249_004014"
     CDS             complement(2554..2919)
                     /locus_tag="OO249_004014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638108.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGG
                     MYCPECGTVHWPDGVAPPF"
     gene            3004..3333
                     /locus_tag="OO249_004015"
                     /pseudo
     CDS             3004..3333
                     /locus_tag="OO249_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3363..3446
                     /locus_tag="OO249_004016"
                     /pseudo
     CDS             3363..3446
                     /locus_tag="OO249_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            3446..3541
                     /locus_tag="OO249_004017"
                     /pseudo
     CDS             3446..3541
                     /locus_tag="OO249_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          949 a          952 c          856 g          894 t
ORIGIN      
        1 aaggtgaaat ctgtcaatca gtaccggctt acgccgggct tcggcggttt tactcctgtg
       61 ccatatgcaa caacggagtg ccgccttcca tgccgctaac gcggcatcaa aaaatccctc
      121 ttctccagtt gcccccctcc gaactcagag tgtattttca tttattgatg aaatttactg
      181 tttgcgcaat tttcctgttt tagtgtattt tgtcattctg gaggctataa tatgtttaat
      241 gtgattactc acccggccgc actggatgaa cttctagaat taccagacaa tctgcgtggt
      301 cgtatgacca gactgattga acggctggaa caggaaggta acaaaatgaa aatgcctcac
      361 agccgtgtaa ttggcggagg gctttttgaa ctgagagtcg gggataaaaa tatcgcaagg
      421 acactgtacg cttatgcaac gggtaacgaa atctatctgc tgcatgcatt tgtgaaaaaa
      481 acgcaaaaga caccaacgaa agctattgaa attgccagga tgcgtctgaa ggagatgaac
      541 tgatgaaagt aaaaggtgtt ccattcagcc aggtaaagca aaaactactc agtactccag
      601 aagctgtcaa agggtatgag gatgctgaca aagaactgga gatggtggaa atgttatacg
      661 aaatgagaga aaaagcggga ttaacaaagt cagcactggc cgaacgaatg gggttacgcc
      721 cgtcagccat cagtcgcctg gaaagtaacc ctttaggggc cagcatgaaa actttgacaa
      781 aatatgctca tgcctgtggc gcaaccatcg atattcacgc tgtctattga ggggtccgct
      841 tggaattcgg aaaataaacc acgttaagac tattttttag ataaatgact ttcaggaatc
      901 gccaagatgg cgggataaca gcggttttgg tgtcctaaat cgtacaattc tgtacatcca
      961 cgcaagggaa tgtacggaag taagccagac ggttatcgga cggtcagaac atttcgtaca
     1021 gcctttacgt tcagtgagtt agctggcctg gacgtaacca ctcgttagct caattctctt
     1081 agcgtgggaa aatttccgaa ttccaagcgg acccctttat ccttgccccg ctgaatgagg
     1141 aaacgctgtt ccgggggatt atgctgaacg tcttccgttc atcgatagga attaaaaccc
     1201 caaaaagatt aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctcatatg
     1261 aacggaaccg acgattaaac tggatggctc tgattgattc agggtatgaa tggcggtttt
     1321 ttgctccgtt tccctcaaaa tggacgctac ttaccctctg cggctctcag ccgcaccacc
     1381 gcatccgggc cagcggctca tgcatcagga cctgctctgc cagacggtag ccccgcttca
     1441 gccccgtaaa acgcatctga ctcccgcaca gcacgcactt cagcgggtca accttcagta
     1501 acctctgata catccctctc caggtgattt gcatcgccgt ttttctcact gtctccgtta
     1561 tgatgtacac cacttcttcc agtaaccgcc gtttcgccgg actcagaaaa ccgtagtacc
     1621 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccatg aactcatctc
     1681 cactcatcaa caggtattct tcccgttttg ttcggtgact gttgtaacgc agtccgattt
     1741 catcctgacc ggcataatgc tccagacgac tcaccggcac cggtggcttt ttcaggtaag
     1801 agccaaagta caccgccaca tgggtggcat tatccatcac ccgggatacg ttgacattcc
     1861 agccacggcg gtaatgcgtg tccaggaagc gattccattc ccgtttactg cttccttctg
     1921 ctgccagcgc atccggcatc accaggtcag ggtatttccg tgacagtaac cgtgttatcc
     1981 ggtagcgcca catgctcatc accttacggg cgtaaaaatg aagatttttc caggtgtggc
     2041 ccgacgtcac accaccggca gttgtcgata aatggatatg cggatgccac tgctggtcac
     2101 gcccccatgt gtggatcacc gtgaatatcc tcggctccac atctgcctga tggcagattt
     2161 ccagtatcac atccgctgca atgcggctca tctctgccag caaccaccgg ttgtggaaca
     2221 ccagggacca gtactggcag ggtaacgtga acacaatatg ctgccatggg cagtcgggga
     2281 ccaggctcag cagatactgt atccactgtg cgccagcctt caccccgcag tgcgggcagg
     2341 agcggctttt acaccggaag cagacctttt ttgtatggca acagtccggt gatgaacagc
     2401 accactgtgt ataccccatc agtgtggtcc cgcacgccat gattttggtc accgactcaa
     2461 tcaccaccgg acgtactgcc ccttccggct gcttctccag ccagttaagc cagcggtttc
     2521 cctgctgaaa gatatcggca aaacggggaa gcatcagaag ggcggggcga ctccgtccgg
     2581 ccagtgaacc gtgccacact ccgggcagta cataccgccg gcgctgatac cggaaagaat
     2641 ggtcgcaaat tcccgctccg tgcagcgggc aatttccgga tacccttcgt catcaacacg
     2701 tacaaaccag aagaccagtt ttttgtttcc ggcatccaca aagaacggaa tattcaggtc
     2761 tgcgcaacac tcaacggcat catcaaaact atcaaagcgc agaacttctg cgtcttcttc
     2821 gtcaaaaaaa tcatcttcgt gaagcttcac gacatagcgg ggaagtttgc ttctttgaga
     2881 ggcgggttta cgtttacggg gtttagctga acgggccata taaccacctg aaagacaatg
     2941 acattgcctg tttttataac ggtaattgca gaccatgaca agccgcagcc gtcaggctgc
     3001 ctactcgagc ttcagctcaa taaaggcgaa tatcgacata aattaccccg atggatcttt
     3061 tttgcaaatc agggtgaatt cacgaccggt gattacgaag aaatcatgaa taaggccagc
     3121 gccctgagtc tggtatcgaa tgcaatcctg tactggaata catcaagaat aagcaatatt
     3181 gttgaagggt tacgtgaaaa tggtgaggtt attgatgatg acgtattagc ccatatatca
     3241 ttactgccct ataaacacgt attacctaat ggtacttatt ttgttgatgg agaaccttac
     3301 ggtactgagc ctaccgaaag agcaaaaccg taagtttctg ttcaaatgct cccgctggtc
     3361 catccggaca gattatcgac catcaggctg tacaggcctg ggcggacagc ctcagtactg
     3421 acaatccgtt accggtgcca cgctgatgga acagaagtgg accagtaagg cgctttctga
     3481 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaatggt
     3541 acacgtatag gaaattgaaa aaccagacga taccgctgtc cacgccttat gccacgtgag
     3601 cttttctgct tttccctcca ctccggtgtt ttctctctct gcatggtcga t
//
LOCUS       DAJTOR010000258         3532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTOR010000258 DAJTOR010000000
VERSION     DAJTOR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.262"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..136)
                     /locus_tag="OO249_004018"
     CDS             complement(<1..136)
                     /locus_tag="OO249_004018"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5638109.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     A"
     gene            complement(277..2310)
                     /gene="metG"
                     /locus_tag="OO249_004019"
     CDS             complement(277..2310)
                     /gene="metG"
                     /locus_tag="OO249_004019"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5638110.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQESRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2442..>3532
                     /gene="apbC"
                     /locus_tag="OO249_004020"
     CDS             2442..>3532
                     /gene="apbC"
                     /locus_tag="OO249_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5638111.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHCLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGE"
BASE COUNT          852 a          897 c          928 g          855 t
ORIGIN      
        1 cagcatctga aaaacttttt atctggcttt gccctttggt gccaactttt ccccaactga
       61 tatgcaactc gtttccctgt tgctcaactg cccagaattt atgtgatttt tcgtcctgat
      121 agataaagtg tctcattctg catccctgta attttatcca gttgaatgca gatgctacca
      181 gtatttatgc gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa
      241 gaaaaggcgc tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt
      301 agcaccggca tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac
      361 catgccttca gagataccga agcgcatttt acgtggtgct aggttagcca ccataatggt
      421 gtgacgacca atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt
      481 gcgtttttca ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa
      541 ctctgcgttt tcaatcagcg ccacgcgcag gtcaacttta gcgaagtcgt caaaggtgat
      601 ggtttcctga atcggatcat ctgccagcgg gccagttacc ggcgcggcag cggcttttac
      661 ttcttcttta gaggcttcca ccagtgcttc aacctgcctc atatcgatgc ggttatacag
      721 cgccttgaac ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag
      781 ttccgtattg aggaatgctt ctgcacgctc ggtcagtttc ggcagtaccg gcttcaggta
      841 agtcatcagc acgcggaaca ggttgatgcc cattgagcaa attgcctgca ggtcggcatc
      901 gcggctttcc tgtttcgcca ccacccacgg agcctgttca tcgacatagc ggttagccag
      961 atcagccagc gccatgattt cgcgcacggc tttaccaaat tcacggcttt cccacgcttc
     1021 accaatcact tcagcggcat cagtgaaggt tttgtacaac tgcgggtcag ccagttcgct
     1081 tgccagcacg ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac
     1141 cactttgtta acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat
     1201 atcatcgatg cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt ctgcgtcaaa
     1261 atgattcagc caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc
     1321 gttcaccgtc acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc
     1381 cagcatggca ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta
     1441 cagctcggcg gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt
     1501 gtcgcacaga ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa
     1561 atatttgccc ggcgcgttcg gaatttcaaa accgaagtaa ggtgcgtcgc gggagatatc
     1621 ccactgttgc aggccagatt caaaccactc ctgcattttg tttgccacct gctcctgcaa
     1681 cgcaccgctg cgggtccatg cctgcaacat ttcgctgaaa gagggcagat caaagaagaa
     1741 gtgttcagaa tcacgcatta ccggggtagc gccagaaacc accgatttcg gctcgatcag
     1801 ttcagtcggg ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga
     1861 tttacatttc gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg
     1921 atcgtacagc tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta
     1981 gataagttct gacaactggc ggttctcttc gctgtgtgtc gagtgatagt tgtcatagct
     2041 gatgttaaag cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc
     2101 cggggtgata ccaagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc
     2161 gcagatgaag ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg
     2221 gatgtgctcc agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt
     2281 caccagaatt ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg
     2341 ggttagatag taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa
     2401 actatcagct gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg
     2461 ccaaatcacc agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc
     2521 caaccctgaa gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg
     2581 acacgttgca tgtggaactg gttatgccat tcgtctggca tagcgcgttt gaagagttaa
     2641 aagagcaatg tagcgccgaa ctgctgcgta tcaccggggc gaaagctatc gactggaaac
     2701 tgtcgcacaa tatcgccacc ttaaaacgcg tgaagaatca gccgggtatt aatggtgtga
     2761 agaacatcat tgcggtcagt tccggtaaag gcggcgtggg taaatcctcc acggcggtaa
     2821 acctggcgct ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct
     2881 acggcccatc aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg
     2941 gtacccatat ggcaccgatt atgtctcatt gcctggcaac caattcaatt ggttatctgg
     3001 tcactgacga caatgcgatg gtgtggcgtg gaccgatggc gagcaaggcg ttaatgcaga
     3061 tgttgcagga aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca
     3121 ccggtgacat tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca
     3181 ccaccccgca ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag
     3241 tcgaggttcc ggtgcttggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg
     3301 gtcaccatga accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccaca
     3361 cacagctgct ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca
     3421 cgccaacggt tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg
     3481 accgcgttgc agctcagctc tactggcagg gtgaagtcat tccaggcgag at
//
LOCUS       DAJTOR010000259         3530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTOR010000259 DAJTOR010000000
VERSION     DAJTOR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.263"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..510
                     /locus_tag="OO249_004021"
     CDS             <1..510
                     /locus_tag="OO249_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5638112.1"
                     /translation="SGFELNGKGTSTRPTLTVSNLYGMVAGMAEDLQSLVGGTVVRRK
                     VYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDGAVFPG
                     RIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNTGNFGG
                     FLSINKLSQ"
     gene            515..1258
                     /locus_tag="OO249_004022"
     CDS             515..1258
                     /locus_tag="OO249_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5638113.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            1195..1797
                     /locus_tag="OO249_004023"
     CDS             1195..1797
                     /locus_tag="OO249_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5638114.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1858..>3530
                     /locus_tag="OO249_004024"
     CDS             1858..>3530
                     /locus_tag="OO249_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5638115.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFSVQALVENTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMVWCR
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDAQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSCTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSV"
BASE COUNT          754 a          883 c         1153 g          740 t
ORIGIN      
        1 agtggttttg aactgaatgg caaaggcacc agtacgcgcc ccacgctgac ggtttctaac
       61 ctgtacggta tggtcgccgg gatggcggaa gatctgcaga gtctggtcgg cggaacggtg
      121 gtccggcgta aggtttacgc ccgttttctg gatgcggtga acttcgtcaa cggaaacagt
      181 gacgccgatc cggagcagga ggtgatcagc cgctggcgca tcgagcagtg cagcgaactg
      241 agcgcggtca gtgcctcttt tgtgctggcc acaccaacgg agacggatgg cgcggttttc
      301 ccggggcgta tcatgctggc gaatacctgt atgtggacct accgttctga tgagtgtggt
      361 tacacgggca gggcagtggc tgacgagttc gacaaaccaa cgacggatat ccggaaggac
      421 aaatgcagca agtgtatgcg cgggtgtgag ttgcgcaaca ataccggtaa tttcggcggt
      481 ttcctttcca tcaataaact ttctcagtaa atccatgaca cagacagaat cagcgattct
      541 ggcgcacgcc cggcgatgtg cgccagcgga gtcgtgtggc ttcgtggtga gaacgccgga
      601 gggggagcgg tatatccctt gtgtgaatat ttctgcagag ccggaggcgt attttcgtat
      661 cgcaccggaa gactggctgc gggcagagat gcagggggag attgtggcac tggtccacag
      721 tcatcccggt ggtctgccct ggctgagtga ggctgaccgg cggctgcagg tgcagagtga
      781 tttgccgtgg tggctggtct gccggggggc gattcacaag ttccgctgtg tgccgcatct
      841 caccgggcgg cgctttgagc acggggtgac ggactgttac acgctgttcc gggatgctta
      901 tcatctggcg gggattgaga tgccggattt tcatcgcggg gatgactggt ggcgtcacgg
      961 tcagaatctc tatctggata atctggaggc cacagggctg tatcaggtgc cgttgtcatc
     1021 agcacaaccg ggcgatgtgc tgctgtgctg ttttggttca tcggtgccga atcatgccgc
     1081 catttactgc ggcgacggtg agctgctgca ccatattcct gaacaactga gtaaacgaga
     1141 gaggtatacc gacaaatggc agcgacgcac acactccctc tggcgtcacc gggaatggca
     1201 cgcatctgcc tttacgggga tttgcaacga tttggccgcc gcatcgacct tcgtgtgaaa
     1261 acgggggctg aagccatccg cgcactggcc acacagctcc cggcgtttcg tcagaaactg
     1321 agtgacggct ggtatcaggt gcgcattgcc gggcgtgatg caggtgaaac cgaattgtct
     1381 gcccgtctta atgagccgct ggcaaatggt gcagtgatcc acatagtacc gcgtctggtg
     1441 ggagctaaaa gtggcggtgt gtttcaggcg gtgctggggg cagctgttat ggcggttgct
     1501 atatggatgc cgggggtagg aattatggcg agtaatctgc tgttttctct cggtgccagt
     1561 atgacgcttg gcggtgttgc acagatgctg gcaccgaaag ccagaactcc ccgtacacag
     1621 acaacggata acggcaaaca gaacacctgt ttctcctcac tggataacat ggttgcccag
     1681 ggcaatgttc tgcctgttct gtacggtgaa atgcgcgtgg ggtcgcgggt ggtatctcag
     1741 gagatcagca cggcagacga aggggatggt ggtcaggttg tggtgattgg tcgctgatgc
     1801 aaaatgtttc atgtgaaacc gcctgcgggc ggttttgtcg tttatggagc gtgaggaatg
     1861 ggtaaaggca gcagtaaggg gcataccccg cgcgaagcga aggacaacct gaaatccacg
     1921 cagttgctga gtgtgattga tgccatcagc gaagggccgg ttgacggtcc ggtggatgga
     1981 ttaaaaagcg tgctgctgaa cggtacgcca gtcctggaca gcgaggggaa gacaaacttt
     2041 tccggtgtta cggtggtgtt ccgcgccggc gagcaggagc agacaccgcc ggaggggttt
     2101 gaatcttccg gctcagagac tgtgctgggt acggaagtga aatacgacac gccgatcacc
     2161 cggaccatca cgtcggcaaa cattgatcgt ctgcgcctga ccttcagtgt gcaggcactg
     2221 gtggaaaaca cctcaaaggg ggaccggaat ccgtcggaag tccgcctgct ggttcagata
     2281 cagcgtaacg gtggctgggt gacggaaaaa gacatcacca ttaagggcaa aaccacttcg
     2341 cagtatctgg cctcggtggt ggtgggtaac ctgccgccgc gcccgtttaa tatccggatg
     2401 cgcaggatga caccggacag caccacagac cagctgcaga acaaaacgct ctggtcgtcg
     2461 tacaccgaaa tcatcgatgt gaaacagtgc tacccgaaca cggcgctggt cggcgtgcag
     2521 gtggattcag agcagttcgg taaccagcag gtgagtcgca attatcatct tcgcgggcgc
     2581 attctgcagg tgccgtcgaa ctataacccg cagacgcggc aatacagcgg tatctgggac
     2641 ggaacgctta agccagcata cagcaacaac atggtctggt gtcggtggga catgctcact
     2701 catccgcgct acggcatggg gaaacgtctt ggtgcggcag atgtggacaa atgggcgctg
     2761 tatgtcatcg gccagtactg cgaccagtca gtgccggacg gatttggcgg cacggagccg
     2821 cgcatcacct gtaacgctta cctgaccaca cagcgtaagg cgtgggatgt tctcagtgat
     2881 ttctgctcgg cgatgcgctg tatgccggta tggaacgggc agacgctgac gttcgtgcag
     2941 gaccgaccgt cggataaagt gtggacctat aaccgcagta atgtggtgat gccggatgat
     3001 ggcgcgccgt tccgctacag cttcagcgcc ctgaaggacc gccataatgt cgttgaggtg
     3061 aactggattg atccggacaa cggtcatgag acggcgacag agcttgtgga ggacgcgcag
     3121 gccattctcc gttacggtcg taacgtcacg aagatggatg cctttggctg taccagccgg
     3181 ggacaggcgc accgcgccgg gctgtggctg attaaaacgg aactgctgga aacgcagacc
     3241 gtggacttca gcgtcggcgc agaagggctt cgccatgtac cgggtgatgt cattgaaatc
     3301 tgcgatgatg attatgccgg tatcagcacc ggtggtcgcg tactggcggt gaacagccag
     3361 acccggacgc tgacgctcga ccgtgaaatc acgctgccgt cctcctgtac cacgctgata
     3421 agcctggttg acggaaatgg caatccggtc agcgtggagg ttcagtccgt caccgacggc
     3481 gtgaaggtga aagtgagccg ggttcctgac ggcgttgcag aatacagcgt
//
LOCUS       DAJTOR010000260         3519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTOR010000260 DAJTOR010000000
VERSION     DAJTOR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.264"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            392..1111
                     /locus_tag="OO249_004025"
     CDS             392..1111
                     /locus_tag="OO249_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5638116.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVGHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            1122..2549
                     /locus_tag="OO249_004026"
     CDS             1122..2549
                     /locus_tag="OO249_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5638117.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKNKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2542..3237
                     /locus_tag="OO249_004027"
     CDS             2542..3237
                     /locus_tag="OO249_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5638118.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
BASE COUNT          922 a          738 c          965 g          894 t
ORIGIN      
        1 atacaaacta aattgcttca attaaataaa atcccgacta ttacttgata cgtgattatt
       61 gtcggtgatt ttgtttgtac accatcagtg tatatctcaa taacccctga ataagtagct
      121 ctgaataggt ataagggatg tggccatttt taaatggatt tcttatgcca aaaatgatcg
      181 tcgatttctc cataaaatgt gagcgatgcc gaaagaaata aaattagtta ttgcatttga
      241 cgtttgggtg aaagattatc atttgtcata caaatgaggg gtagtatgtt gctagccaat
      301 taaaaaagaa cgccatattt attgatgatt gatcccccgg tgggggggaa acatcgggca
      361 taaatgggca tgaagtaatg gagtattagt tgtgaatgtc aatttctttg tcacctgtat
      421 tggtgacgcc ctgaaatcaa gaatggcacg agactccgtg ctgctactgg aaaaactcgg
      481 ctgtcgcgta aatttcccgg agaaacaggg atgctgcggt cagcctgcga tcaatagcgg
      541 ttatatcaaa gaagcgattc cagggatgaa aaatctgatc gccgcactgg aggataacga
      601 cgatcccatt atttcaccgg ctggctcttg cacctatgcc gtaaaaagtt acccgacgta
      661 tctggcggat gaacctgaat gggcatcacg tgccgaaaag gttgccgcgc gtatgcagga
      721 tctcacctct tttattgtta ataaattagg ggtagtcgat gtaggtgcca gtttgcaagg
      781 gagagctgtg tatcacccat cttgtagcct ggcccgtaag ctgggagtga aggacgagcc
      841 acttacgctg ctgaaaaatg tgcgtggact ggagctgttg acctttgctg aacaggatac
      901 ctgctgcgga tttggcggca cgttctcggt caaaatggcc gaaatatccg gcgagatggt
      961 gaaagaaaag gttgggcacc tgatggaagt ccgccctgag tatttaattg gtgctgacgt
     1021 gagttgcctg ctgaacatca gtgggcgatt acaacgggaa gggcagaaag tcaaagtgat
     1081 gcatattgct gaagtgttga tgagccgctg aggatataaa gatgtcgatc aaaaccagta
     1141 atacagattt taagactcgc atccgtcagc aaattgaaga tccgatcatg cgcaaagcgg
     1201 tggcaaacgc gcagcagcgt attggggcaa atcggcaaaa aatggtcgat gaattggggc
     1261 actgggagga gtggcgcgat cgggccgccc agatacgtga tcatgttctg agtaatctcg
     1321 acgcttatct gtaccagctc tcagaaaaag tgacgcaaaa cggcggtcac gtctggtttg
     1381 caaaaaacaa agaagacgct acccgctaca ttttacaggt tgcccaacgc aaaaatgccc
     1441 ggaaggtggt gaaatctaaa tcgatggtga ccgaagagat tggtgtcaat catgtgttgc
     1501 aggatgctgg cattcaggtg attgaaaccg atctgggtga atatattctc cagctggatc
     1561 aagatccgcc atctcatgtt gtggtcccgg caattcataa agatcgccat cagatccgtc
     1621 gagtgctaca cgaacgtctg ggctatgagg ggccggaaac gcctgaagcg atgaccttat
     1681 tcatccggca aaaaatccgc gaagatttcc tcagtgctga aataggtatt accggctgta
     1741 atttcgcggt ggcagagaca ggttcggtat gcctggtgac caatgaaggt aatgcgcgaa
     1801 tgtgtaccac gctgcctaaa acgcatattg cagtgatggg aatggagcgt attgccccca
     1861 cgtttgccga ggtagatgta ttgatcacca tgctggcgcg cagtgccgtt ggtgcacgtt
     1921 tgacgggata caacacctgg ctgacaggac cgcgcgaagc ggggcacgtt gatggtcctg
     1981 aagagtttca tctggttatt gtcgataacg ggcgttctga ggtgctggcc tctgaatttc
     2041 gggatgtgct gcgctgtatt cgctgcgggg cttgtatgaa tacttgtccg gcatatcgcc
     2101 atattggcgg tcatggatat ggctctattt atccagggcc aattggtgcg gtgatttctc
     2161 cgctacttgg cggctataaa gattttaaag atttacccta cgcctgctct ttatgcactg
     2221 cttgtgacag cgtgtgtccg gtgcgtattc cgctgtcaaa actgattttg cgtcatcgtc
     2281 gggtgatggc tgaaaaaggg atcaccgcaa aagcagagca acgggcgata aaaatgttcg
     2341 cttatgccaa tagtcatcca ggattgtgga aagtcgggat gatggccggc gctcatgcgg
     2401 caagctggtt tatcaatggc ggcaaaacac cactcaaatt tggcgcgatt agcgactgga
     2461 tggaagcacg cgatcttcct gaagctgacg gagagagttt ccgtagttgg tttaagaaac
     2521 atcaggcgca ggagaaaaag aatggataat cgaggcgaat ttttgaataa cgttgctcag
     2581 gcactgggtc gcccgctgcg acttgaaccg caagcagaag atgcgccgct taacaactat
     2641 gctaacgagc ggcttaccca acttaaccaa cagcagcgct gtgacgcgtt tattcagttt
     2701 gccagcgatg ttatgttgac gcgctgtgag ctgaccagcg aggcgaaggc ggcagaagct
     2761 gcaatacgtc tgtgtaaaga gctgggagat cagtcggtcg tgattagcgg tgacacgagg
     2821 ctggaggaat tggggattag tgaacgtttg cagcaggaat gcaatgccgt tgtttgggat
     2881 ccggcgaaag gtgccgagaa tatctcgcag gcagagcagg ctaaagtggg tgttgtgtat
     2941 gctgaatatg gtttaaccga atcgggaggc gtggttcttt tttccgccgc cgagcgcggg
     3001 cgttcattga gcctgctccc ggaatcttct ctttttatcc tgcgtaaaag cacaatcctg
     3061 ccgcgtgtag cgcaactcgc agaaaaattg catcagaaag cgcaggccgg tgaacgaatg
     3121 ccgtcctgca ttaacatcat tagcggccct agttcaacgg cggatattga gcttatcaaa
     3181 gtcgtcgggg ttcatggccc ggtgaaagcg gtgtatctga ttattgagga ttgctgagag
     3241 agatatgaga tctgaacata aacctgaaga aggcatacga ggttgaaaat agcgttaagg
     3301 acaacctgac ttaaccccgt tccgtcatgg ttatgctgct cttctgtaaa tatcctggca
     3361 ttagtaatgc caggatattt acagtgaaaa agacaggcat ccattcctga aaacgggttc
     3421 ccttcccggt cattgtcact tcagctttat acaggcactc tctatcagaa tgttgtttat
     3481 tactgcccag agttttgttg gtattcatct accattttt
//
LOCUS       DAJTOR010000261         3487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTOR010000261 DAJTOR010000000
VERSION     DAJTOR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.265"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..1948
                     /locus_tag="OO249_004028"
                     /pseudo
     CDS             1..1948
                     /locus_tag="OO249_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            complement(1991..2437)
                     /gene="ivy"
                     /locus_tag="OO249_004029"
     CDS             complement(1991..2437)
                     /gene="ivy"
                     /locus_tag="OO249_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5638119.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            2618..3388
                     /gene="yafV"
                     /locus_tag="OO249_004030"
     CDS             2618..3388
                     /gene="yafV"
                     /locus_tag="OO249_004030"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5638120.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT          778 a          892 c         1001 g          816 t
ORIGIN      
        1 gatgattatc aaaaaagagt acggctgact ggagttctca gcttatgccc agtctcgcgt
       61 gctgcaaaaa ctctccggcg tgagcgggat cctggcgatt accgtcggcg tgccgaactc
      121 attaggcccg ggcgagctgc tgcaacatta cggcactgac gagcagaaag atcactatct
      181 gccgcgtctg gcgcgtggtc aggagatccc ttgctttgca ctgacaagcc cggaagcggg
      241 ttccgatgcg ggcgcgattc cggacaccgg gattgtctgc atgggcgaat ggcagggcca
      301 gcaggtgctg gggatgcgtc tgacttggaa caaacgctat attacgctgg caccgattgc
      361 gactgtgctt gggctggcgt ttaaactctc cgacccggaa aagctgctgg gtggcgcaga
      421 agatttaggt attacctgtg cgctgatccc gaccaccacg ccgggcgtgg aaattggtcg
      481 tcgccacttc ccgctgaacg taccgttcca gaacggaccg acgcgcggta aagatgtctt
      541 cgtaccgatc gattacatca tcggtgggcc gaaaatggcc gggcaaggct ggcggatgct
      601 ggtggagtgc ctctcggtag gccgcggcat caccctgcct tccaactcaa ccggcggcgt
      661 gaaatcggta gcgctggcaa cgggcgcgta tgctcacatt cgccgtcagt tcaaaatctc
      721 tatcggaaag atggaaggga ttgaagagcc gctggcgcgt attgccggta atgcctacgt
      781 gatggatgct gcggcatcgc tgattaccta cggcattatg ctcggcgaaa aacctgccgt
      841 gctgttggct atcgttaagt atcactgtac ccaccgcggg cagcagtcga ttattgatgc
      901 gatggatatc actggtggca aaggcattat gctcgggcaa agcaacttcc tggcgcgtgc
      961 ttaccagggc gcaccgattg ccatcaccgt tgaaggggct aacattctga cccgcagcat
     1021 gatgatcttc ggacaaggag cgattcgttg ccatccgtac gtgctggaag agatggaagc
     1081 ggcgaagaac aatgacgtca acgcgttcga taaactgttg ttcaaacata tcggtcacgt
     1141 cggtagcaac aaagttcgca gcttctggct gggcctgacg cgcggtttaa ccagcagcac
     1201 gccaaccggc gatgccacta aacgctacta tcagcacctg aaccgcctga gcgccaacct
     1261 cgccctgctt tctgatgtct cgatggcagt gctgggcggc agcctgaaac gtcgcgagcg
     1321 catctcggcc cgtctggggg atattttaag ccagctctac ctcgcctctg ccgtgctgaa
     1381 gcgttatgac gacgaaggcc gtaatgaagc cgacctgccg ctggtgcact ggggcgtaca
     1441 agatgcgctg tatcaggctg aacaggcgat ggatgattta ctgcaaaact tcccgaaccg
     1501 cgtggttgcc gggctgctga atgtggtgat cttcccgacc ggacgtcatt atctggcacc
     1561 ttctgacaag ctggatcata aagtggcgaa gattttacaa gtgccgaacg ccacccgttc
     1621 ccgcattggt cgcggtcagt acctgacgcc gagcgagcat aatccggttg gcttgctgga
     1681 agaggcgctg gtggatgtga ttgccgccga cccaattcat cagcggatct gtaaagagct
     1741 gggtaaaaac ctgccgttta cccgtctgga tgaactggcg cacaacgcgc tggcgaaggg
     1801 cctgattgat aaagatgaag ccgctattct ggtgaaagct gaagaaagcc gtctgcgcag
     1861 tattaacgtt gatgactttg atccggaaga gctggcgacg aagccggtaa agttgccgga
     1921 gaaagtgcgg aaagttgaag ccgcgtaatt gaataacgga gccgaaaggc tccgtttctt
     1981 tatccgctaa ttatttaaaa ttaaagccat ccggatggtt ttccaggctg ccggtcaacg
     2041 ccgcgaacag caccgtttta ccatcaatcg aaagcgcatc gttcacattc agccaggtga
     2101 gtttctcttg cgacgttttc tcatcaatag tcgagaacag ccccgtcatc tgattagatt
     2161 tctcggacca catcacagcg atacgttgcg agccacagtc atgcggtttg cacgcgctca
     2221 tcacctgata cgtctcatct cccaacgtta cggtttgtgc gggagtataa gtaccgcctt
     2281 tcatcaccca ggcaggcagc ttatgccctt gtaccatctg attaaatgca gctttggtgg
     2341 tttcgccctt tgcaaggctg ctaatggtta aatcatcctg cgccattgca ctggtggcga
     2401 tgaccagagc ggcgactgtc gttattgcct taaacatcat tcctcccgag cttatcctgc
     2461 ccatgttatt aaccttctaa agcctggcga taacacgata atttgcaaat cagatatttc
     2521 caatatcagc atttgttatc aatttattct gcgctaaggc aactgcctga taaggtgtta
     2581 aagttataaa acccttcgca taaaggagtt gtacaccgtg cctggtttga agattacgct
     2641 tttgcagcaa ccactggtgt ggatggatgg ccctgccaac ctgcgtcatt ttgatcgtca
     2701 actggaaggt attaccgggc gcgatgtgat cgttctaccg gagatgttta ccagcggctt
     2761 tgccatggaa gcggcagctt cgtcgctagc acaagatgac gtagtgaact ggatgacagc
     2821 taaggcgcag cagtgcaatg cgctgatagc aggcagtgtt gcattacaaa cggagtctgg
     2881 ttcggttaac cgctttttgc tggttgagcc gggcggcacg gtacattttt atgataagcg
     2941 tcatctgttc cgcatggcag atgagcatct acattataaa gcgggcaatg cgcgagtgat
     3001 tgtggaatgg cgcggctggc gtattttgcc gttggtgtgc tacgacttac gttttccggt
     3061 gtggtcgcgc aatctcaacg attatgacct cgccatctac gttgccaact ggcctgctcc
     3121 gcgctctctg cactggcagg cattgctgac ggcccgcgcg attgagaatc aggcgtatgt
     3181 ggcgggatgc aatcgcgtcg gcagcgatgg caacggctgc cattatcgcg gtgacagccg
     3241 ggtgattaat ccacaaggcg agattatcgc tactgccgac gcgcatcagg caacgcgcat
     3301 tgatgcggag ctgtcgatga tggcattgcg ggaatataga gaaaagtttc cggcatggca
     3361 ggatgcggat gagtttaggt tgcggtgagt gggggttgtg tttaaggctg ggggagagtc
     3421 ggggtaagac tacgaaagcc cgctctccgc aaggactgac gccagatagt ttctgtccat
     3481 agctgct
//
LOCUS       DAJTOR010000262         3432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTOR010000262 DAJTOR010000000
VERSION     DAJTOR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.266"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(118..2187)
                     /gene="glyS"
                     /locus_tag="OO249_004031"
     CDS             complement(118..2187)
                     /gene="glyS"
                     /locus_tag="OO249_004031"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5638121.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLVESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2197..3108)
                     /gene="glyQ"
                     /locus_tag="OO249_004032"
     CDS             complement(2197..3108)
                     /gene="glyQ"
                     /locus_tag="OO249_004032"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5638122.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3203..>3432)
                     /locus_tag="OO249_004033"
     CDS             complement(3203..>3432)
                     /locus_tag="OO249_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5638123.1"
                     /translation="QKAQRVKVDPLRSLNMEALCKDQAAKRYNTGEQKIDVTAFEQFQ
                     GSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
BASE COUNT          776 a          949 c          898 g          809 t
ORIGIN      
        1 cataaaatta atatatgaga taatctttat tcagcagaag attattaaag gttgctgtat
       61 tatttagcga taataaaaaa agcctgccag atggcaggct ttttaataac ggcgttatta
      121 ctgcaacagc gaaatatccg caacgcgcag gaacagttcg cgcagtttct ccagcatggt
      181 cagacggttg agacgcaatt ctttgtcatc aaccatgacc atcactttat cgaagaaagc
      241 atcaaccggc tcacgcagct ctgccagttc gaccagcgca tcctggtaac gaccttccgc
      301 aaagtacggc tccagcttgt cacgtagcac cacaacctgc atcgccagtt taatttcttc
      361 cggctctttc agggtagagg cattcacgcg gtcgctcagc acttcgtcag atttcgccag
      421 aatgttagat acacgtttgt tcgccgccgc cagtgcagca gctgcatcca gggtacggaa
      481 atgcgatacc gctttcatac gggcatcgaa atcagccgga cgagtcggac gacgcgccag
      541 taccgcctgg atggtgtcaa cggtgtaacc ttcgtcctga taccaggcgc ggaagcgacc
      601 gagcataaag tcgataacat catcaaccac gttggcgtta gtcagcttat cgccatacag
      661 acgcactgct tcttcggtca gcgtttgcag atcaaggttg aggttcttct caacgataat
      721 tcgcagcacg ccaagtgcgg cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg
      781 ctgaccgata ccgaagatac ccgccagagt atccatctta tcagcaatcg ccagcgcaca
      841 agctaccggg ttggacggca ggtcatcacc ggcaaagcgc ggctgatact gctcattcag
      901 cgcgacggct acgtcttccg cttcgccatc gtgacgcgcg tagtgcatcc ccataacgcc
      961 ttgggtgtcg gtgaactcga agaccatgtt ggtcatcagg tcacacttag acagcaggcc
     1021 cgcacgggtt gcgtggttaa cgtcagcgcc aatctgttca gcaatccagc cagccagcgc
     1081 ctggatgcgg tcagttttgt cacgcagcgt acccagctgt tgctggaaca gcacggtttg
     1141 caggcgcggc aggttatctt ccagacgttt tttacggtcg gtattgaaga agaactcggc
     1201 atccgccaga cgtggacgaa cgactttctc gttaccagag ataatttgct gcggatcttt
     1261 cgattcgatg ttggcaacga agatgaagtt cggcagcagt ttgccatcgt tcgcatacac
     1321 cgggaagtat ttctggtcac ctttcatggt gtaaaccagg gcttcagccg gcaccgcgag
     1381 gaatttctct tcgaattttg cggtcagaac gaccggccac tccaccagcg aagccacttc
     1441 ttccagcagg ctttcgctta agtcagcgtt accgccaatc ttacgcgctg cttcttcggc
     1501 atcggcttta atcttcgctt tacgttcttc gtaatcggcg atgactttcc cgcgctcacg
     1561 cagaatttcc ggatactgat cggcgttgtc gatggtgaat tccggttcgc ccataaagcg
     1621 gtggccgcga atcacgcgat cggactgaat gcccagaata gttgccggaa tgactttgtc
     1681 gcccagcagc agggtcacgg tgtgcaccgg acgcacgaag tgcacgtcgc ttgcgcccca
     1741 acgcatcagt ttcgggatcg gcagtttcgc cagagaagtc gcaaccatat tcggcagcag
     1801 tgcttcggtg ctttcgccct tcacatgggc gcgatacagc agccattcgc ctttatcggt
     1861 agtcagacgc tcagcctggt caacggtaat accgcaacca cgcgcccaac cttctgccgc
     1921 tttgctcggt ttgccttcag cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat
     1981 ttcacgatcc ggttgcgctt ccgccaggtt agctactttc agcgccagac gacgcggagc
     2041 agcaaaccat tgaacggtgc cgtgtgcgag gccagcgtta tccagctcca cagtaaaatt
     2101 cgcagcaaag gactcaacca ggctgcgcag tgcttttggt ggcagctctt cagtgccgat
     2161 ttccaccaga aaagttttct cagacatagc cgcctcttac ttatctttgt tgcacatcgg
     2221 gaagccgagg gcttcacggg aagcgtagta tgcttctgcc actgctttgg tcagggtgcg
     2281 aatgcgcaga atgtagcgct gacgctcggt gacggagatg gctttacgcg catccagcag
     2341 gttgaagctg tgggcggctt tcagaatacg ctcgtaggct ggcagcggca gcggattttc
     2401 cagcgccagc agctgctgcg cttctttctc gtactgctca aagcaggtga acaggaagtc
     2461 cacatccgcg tattcgaagt tgtaagtgga ctgctccact tcgttctgat ggaacacgtc
     2521 gccgtaggtg gttttaccca gcgggccgtc gctccagacc aggtcgtaaa cgctgtctac
     2581 gccctgaatg tacatggcca gacgttccag accgtaggtg atctcgccgg taaccggttt
     2641 acactccaga ccaccaacct gctggaagta agtgaactgc gtcacttcca tgccgtttag
     2701 ccacacttcc cagcccagtc cccaggcacc cagagtcggg ttttcccagt tatcttccac
     2761 gaaacggatg tcgtgaatag tcgggtccat gcccagctct ttcagagaac cgaggtacag
     2821 ctcctgaata ttgtccggcg atggcttaat gaccacctgg aactgatagt agtgctgtaa
     2881 acggttgggg ttttcgccgt agcgaccatc ggtcgggcga cgagaaggct gaacataagc
     2941 agccgccatc ggttctggcc ccagcgcgcg cagacaggtc attgggtgag aggttcccgc
     3001 gccgacttcc atgtccaatg gttgaacaat ggtgcagccc tggcgagccc agtaatcctg
     3061 taaggtcagg atcaagccct ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc
     3121 tggatacgtg tggatttaaa ggaagggatc agtatacccg ctggatggaa gatatacagt
     3181 acgaaacggg aaaaagcagg gcttaacgca tggaaagatg caaaaaatgg ccatctgcgt
     3241 caaaagaaca gacaaactgc tctttacgga aggtataacc gcgcatttca tagcttccct
     3301 ggaactgttc gaaggcggtg acgtcgattt tttgctcgcc ggtgttataa cgttttgccg
     3361 cctgatcctt gcataacgct tccatattca acgaacgcag aggatcaacc tttacccgtt
     3421 gtgctttctg ca
//
LOCUS       DAJTOR010000263         3411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTOR010000263 DAJTOR010000000
VERSION     DAJTOR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.267"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(21..323)
                     /locus_tag="OO249_004034"
     CDS             complement(21..323)
                     /locus_tag="OO249_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638124.1"
                     /translation="MLEIVLSPVKAQQFTVTLDAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEELI"
     gene            complement(323..1033)
                     /locus_tag="OO249_004035"
     CDS             complement(323..1033)
                     /locus_tag="OO249_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638125.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FIVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(1030..3051)
                     /locus_tag="OO249_004036"
     CDS             complement(1030..3051)
                     /locus_tag="OO249_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5638126.1"
                     /translation="MAQVLDELVLSLGIDDRDFTAGEQAVHAALNRLTTVMEGVADTF
                     SQGQKKTSESLEKTGKDADKTARRMEDAGKRASRFFSGIRSEILALAGVSLTLGGLKN
                     LVTGFARDLNRLSVESDAFGMKARNLDGWLRAARANNVDEGEMSGAFSRLANAKAAFR
                     AGRSFDPVLQDLFQVAARAGISVDLNTDSTEAIMRKLAVIFPRLTKSEQTAYGNALGF
                     SYAGQQFLGSGHALKDVDEFTSRSEVTPGRTALARKLRKELVELDQTWSGIGMTISTA
                     LMPYALKLSHWLETLGDWMQQHPEEVNRFITTFLNDVESVASLANKAAGELGGWQNVI
                     ITLIGLKVASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDWLDSHGFF
                     WASDGTFFFNKKEMETYQTKLDAGEKPGNITRAQSPTVWQQGMLDTQASQATGREAAF
                     GESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPPGLLRSVA
                     ITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKLGGLIRFF
                     HGDLAKALAAYNWGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIRSLHPAPV
                     SQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
     gene            complement(3035..3217)
                     /locus_tag="OO249_004037"
     CDS             complement(3035..3217)
                     /locus_tag="OO249_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5638127.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT          797 a          993 c          815 g          806 t
ORIGIN      
        1 tattttacgt tgtctgtagc tcatatcagt tcctcaggta acagccagta gagccggtac
       61 cgggatccga gcccccgcca gtcagggtca ctattcccct ccgtgtcggt aaaaaacagg
      121 tccccctgaa acggcaggta tccgtaccgg acaatccggt tattattcag acacagcacg
      181 ccatacaggc acggttcacc attaacaatg atatcgatat acatccccgt agtacgctga
      241 ttcaggcgaa tggtgcagac ctgagcgtcc agtgtcaccg taaactgctg ggctttaacg
      301 ggagataaaa caatttccag catcaggtga tccccctgtt cgtggcactt cgtctgtcag
      361 cgtctgacgg ttgtgtcacc gacgccgtaa ctggctgcgt tttaaccgat gcggcccctt
      421 ttgctttatc gttgtccgtg ggagacgact tatccgtact tcccactgac acctctcctg
      481 tattcattac ggtctgaaat accgcagtaa ccgtcagtaa ggtcggacca ttattacttc
      541 gcattcggta gtcatatttc acaaggtcgc aggatgtcca tgtcttgtcc ggcgtctcaa
      601 tatcgtagag tcctgccgtg gtacgcatcc gttcaagcgt ttccagcaca ttcgtccgcg
      661 aggtggtgga aaaatttgtc aggttcggga cggatccgga aaaggccgtc cacccctcta
      721 caatgaaagt cacatgcagt tccggcgatc tctggatttt attaaaggtg gtataagccc
      781 cctgctccac ggggacggtg gaaacagaag cctccgcgcc cacctcaacg acaacaaaag
      841 aatccgggga gaaaggtttg ccacccttct gatgggcacc tgccggatca ttccgtgcgt
      901 aataaatacc gaatgacggt gccagtacac tattaatgag tcccaggaca ccgccgccac
      961 gaactgcact cagtacgtta ctttcactga gcgaaaagtt attcagggaa atattatcga
     1021 aagaaaaact catcctgtta ccccgctgga ataaaccgac acaagcgccg aattcgtgat
     1081 acgccgacgt gcgtcatcgg taataccctt cacattgtcc gacgttgtgg tgacattcag
     1141 tgtcccgata tgcgtggttt ccgttacggt ggactgcgat acaggcgcag gatggagcga
     1201 ccgtatggcc attgccgcac ccggataagg caggttcgcc agcacccgcg gaatatagtt
     1261 gcgggtctcc tccggagcag cagccagtcc cttacgctga acatttccct ctccccagtt
     1321 atatgccgcc agagccttag ccagatcgcc atgaaaaaaa cgtatcaggc caccaagttt
     1381 tctcgcggcg gcatcagcgg ctttttcggg atcaaaggca tcgttccccc tcagaccaaa
     1441 ttcactggcc gtcttcggca tgaactgaaa cagtcccatc gcaccagcag gtgagacggc
     1501 aaactgatta ccacgggatt cggtgatcgc aacgctgcgc agcagtccgg gcggcaggtt
     1561 atatttttcc tccagttgag acagtttcgg ttgcagccag cctaacaggg cttccccggt
     1621 cttcgtcggt cgctggcggt tttgcatggc attaccgagt ttttcctgcg tcgcacgcat
     1681 accctgtagc caggattcac cgaaggctgc ttccctcccg gttgcctgag aagcctgagt
     1741 atccagcatg ccctgctgcc atactgtggg tgattgtgcc cgggtgatgt tgccaggctt
     1801 ttctccggca tccagttttg tctgatacgt ctccatctct ttcttattga aaaagaaagt
     1861 cccgtctgaa gcccagaaaa aaccatgtga atccagccag tccttatttt ccctgccaac
     1921 aatggatgtc agtaatccgt caacaaccgg gtaaagcgcc gttatcgcaa aaagaaggcc
     1981 tccggggcca ctgagggctt ttgtcagccc ccgcacccat gacgccactt tcagcccgat
     2041 cagcgtaata atgacattct gccagccccc cagttctccg gcagccttat tcgccaggga
     2101 ggccactgac tcaacgtcat tcagaaatgt ggtgataaac ctgttcactt cctccggatg
     2161 ttgctgcatc cagtcaccga gtgtctccag ccagtggctg agcttcagcg cgtacggcat
     2221 caacgcggtg ctaatcgtca tcccgatgcc ggaccaggtc tgatcgagct caacaagctc
     2281 ttttcgtagc ttacgggcca gcgctgtacg ccccggcgta acctccgaac gggaagtaaa
     2341 ttcgtccaca tctttcagcg catgaccgga gccaagaaac tgctgtccgg cataactgaa
     2401 gcccagcgcg ttgccgtagg cagtttgttc ggactttgtc agtcgcggaa aaataaccgc
     2461 cagcttgcgc atgatggctt cagtgctgtc agtatttaaa tcaacactaa tgcccgccct
     2521 ggctgcaacc tgaaacaaat cctgtaacac aggatcaaag gaccttccgg ctctgaaggc
     2581 cgcttttgca tttgcaagcc gggaaaatgc cccagacatt tccccttcat caacattatt
     2641 tgcccgcgca gcccgtaacc atccgtccag atttctggcc ttcatgccaa aagcatcgga
     2701 ttccacggac agacggttca gatcacgggc aaaacctgtt accaggttct tcagcccccc
     2761 cagcgtcaga ctgacacccg caagagccag aatctcgcta cggatccctg aaaaaaaacg
     2821 ggacgcccgt ttcccggcat cttccattcg acgggccgtt ttatcagcat cttttccggt
     2881 tttttccagt gattcactgg ttttcttctg tccctgactg aatgtgtcag cgacaccttc
     2941 catcaccgtc gtgagccggt taagggcagc atgtactgcc tgttccccgg cagtaaagtc
     3001 cctgtcatca atcccgaggg acagaacaag ctcatcaagt acctgcgcca tttctgttct
     3061 cctgtatcac ctgttcgtta tgggcatcca catgaattat ttccagcaga tcccataagt
     3121 cctgcacacc aaggacagaa tccagttcag cttttgaagc cttaccggag gagataacgg
     3181 tcgcaatggt acggggaaca ttaacgtaat ccaccacccc gaacggtctg tcggggtcga
     3241 gataacgcgg ggggatatct agctgacgac ggtagcgaaa aaatccacat gcagcttaaa
     3301 gacctccgca cgcagattaa gacgggtaat gatttcttca atatcctctt caacaagcgg
     3361 tctccggatg ctgcgttttt tcggatcggg aacaaactgc acacagtcca t
//
LOCUS       DAJTOR010000264         3365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTOR010000264 DAJTOR010000000
VERSION     DAJTOR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.268"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..297
                     /locus_tag="OO249_004038"
     CDS             <1..297
                     /locus_tag="OO249_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5638128.1"
                     /translation="TKRRELEAFIQQTIQKANKLTPSTTEPKSTRIKQIKTNKKEAVT
                     SERKKRAEHLKPSSSGDEAKVIPFNAVEADDQEDYSLPTYVPELFQDPPEKDES"
     gene            294..1961
                     /locus_tag="OO249_004039"
     CDS             294..1961
                     /locus_tag="OO249_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5638129.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            1964..>3365
                     /locus_tag="OO249_004040"
     CDS             1964..>3365
                     /locus_tag="OO249_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5638130.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAF"
BASE COUNT          968 a          687 c          811 g          899 t
ORIGIN      
        1 actaaacgca gggagcttga ggcgtttatt cagcaaacca ttcagaaagc gaataagtta
       61 acgcccagta ctactgagcc caaatcaaca cgcattaagc agattaaaac taataaaaaa
      121 gaagccgtga cctcggagcg taaaaaacgt gcggagcatt tgaagccaag ctcttcaggt
      181 gatgaggcta aagttattcc tttcaacgca gtggaagcgg atgatcaaga agattacagc
      241 ctacccacat acgtgcctga attatttcag gatccaccag aaaaggatga gtcatgagtg
      301 ctacccggat tcaagcagtt tatcgtgata cgggggtaga ggcttatcgt gataatcctt
      361 ttatcgaggc cttaccacca ttacaagagt cagtgaatag tgctgcatca ctgaaatcct
      421 ctttacagct tacttcctct gacttgcaaa agtcccgtgt tatcagagct cataccattt
      481 gtcgtattcc agatgactat tttcagccat taggtacgca tttgctacta agtgagcgta
      541 tttcggtcat gattcgaggt ggctacgtag gcagaaatcc taaaacagga gatttacaaa
      601 agcatttaca aaatggttat gagcgtgttc aaacgggaga gttggagaca tttcgctttg
      661 aggaggcacg atctacggca caaagcttat tgttaattgg ttgttctggt agtgggaaga
      721 cgacctctct tcatcgtatt ctagccacgt atcctcaggt gatttaccat cgtgaactca
      781 atgtagagca ggtggtgtat ttgaaaatag actgctcgca taatggttcg ctaaaagaaa
      841 tctgcttgaa ttttttcaga gcgttggatc gagccttggg ctcgaactat gagcgtcgtt
      901 atggcttaaa acgtcatggt atagaaacca tgttggcttt gatgtcgcaa atagccaatg
      961 cacatgcttt agggttgttg gttattgatg aaattcagca tttaagccgc tctcgttcgg
     1021 gtggatctca agagatgctg aacttttttg tgacgatggt gaatattatt ggcgtaccag
     1081 tgatgttgat tggtacccct aaagcacgag agatttttga ggctgatttg cggtctgcac
     1141 gtagaggggc agggtttgga gctatattct gggatcctat acaacaaacg caacgtggaa
     1201 agcccaatca agagtggatc gcttttacgg ataatctttg gcaattacag cttttacaac
     1261 gcaaagatgc gctgttatcg gatgaggtcc gtgatgtgtg gtatgagcta agccaaggag
     1321 tgatggacat tgtagtaaaa ctttttgtac tcgctcagct ccgtgcgcta gctttaggca
     1381 atgagcgtat taccgctggt ttattgcggc aagtgtatca agatgagtta aagcctgtgc
     1441 accccatgct agaggcatta cgctcgggta tcccagaacg cattgctcgt tattctgatc
     1501 tagtcgttcc cgagattgat aaacggttaa tccaacttca gctagatatc gcagcgatac
     1561 aagaacaaac accagaagaa aaagcccttc aagagttaga taccgaagat cagcgtcatt
     1621 tatatctgat gctgaaagag gattacgatt caagcctgtt aattcccact attaaaaaag
     1681 cgtttagcca gaatccaacg atgacaagac aaaagttact gcctcttgtt ttgcagtggt
     1741 tgatggaagg cgaaacggta gtgtcagaac tagaaaagcc ctccaagagt aaaaaggttt
     1801 cggctataaa ggtagtcaag cccagcgact gggatagctt gcctgatacg gatttacgtt
     1861 atatctattc acaacgccaa cctgaaaaaa ccatgcatga acggttaaaa gggaaagggg
     1921 taatagtgga tatggcgagc ttatttaaac aagcaggtta gccatgagaa actttcctgt
     1981 tccgtactcg aatgagctga tttatagcac tattgcacgg gcaggcgttt atcaagggat
     2041 tgttagtcct aagcagctgt tggatgaggt gtatggcaac cgcaaggtgg tcgctacctt
     2101 aggtctgccc tcgcatttag gtgtgatagc aagacatcta catcaaacag gacgttacgc
     2161 tgttcagcag cttatttatg agcatacctt attcccttta tatgctccgt ttgtaggcaa
     2221 ggagcgccga gacgaagcta ttcggttaat ggagtaccaa gcgcaaggtg cggtgcattt
     2281 aatgctagga gtcgctgctt ctagagttaa gagcgataac cgctttagat actgccctga
     2341 ttgcgttgct cttcagctaa ataggtatgg ggaagccttt tggcaacgag attggtattt
     2401 gcccgctttg ccatattgtc caaaacacgg tgctttagtc ttctttgata gagctgtaga
     2461 tgatcaccga catcaatttt gggctttggg tcatactgag ctgctttcag actaccccaa
     2521 agactcccta tctcaattaa cagcactagc tgcttatata gcccctctgt tagatgctcc
     2581 acgagcgcaa gagctttccc caagccttga gcagtggacg ctgttttatc agcgcttagc
     2641 gcaggatcta gggctaacca aaagcaagca cattcgtcat gacttggtgg cggagagagt
     2701 gaggcagact tttagtgatg aggcactaga gaaactggat ttaaagttgg cagagaacaa
     2761 ggacacgtgt tggctgaaaa gtatattccg taagcataga aaagccttta gttatttaca
     2821 gcatagtatt gtgtggcaag ccttattgcc aaaactaacg gttatagaag cgctacagca
     2881 ggcaagtgct cttactgagc actctataac gacaagacct gttagccagt ctgtgcaacc
     2941 taactctgaa gatttatctg ttaagcataa agactggcag caactagtgc ataaatacca
     3001 aggaattaag gcggcaagac agtctttaga gggtggggtg ctatacgctt ggctttaccg
     3061 acatgacagg gattggctag ttcactggaa tcaacagcat caacaagagc gtctggcacc
     3121 cgcccctaga gttgattgga accaaagaga tcgaattgct gtacgacaac tattaagaat
     3181 cataaagcgt ctagatagta gccttgatca cccaagagcg acatcgagct ggctgttaaa
     3241 gcaaactcct aacggaacct ctcttgcaaa aaatctacag aaactgcctt tggtagcgct
     3301 ttgcttaaag cgttactcag agagtgtgga agattatcaa attagacgga ttagccaagc
     3361 tttta
//
LOCUS       DAJTOR010000265         3362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTOR010000265 DAJTOR010000000
VERSION     DAJTOR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.269"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..1015)
                     /gene="maeB"
                     /locus_tag="OO249_004041"
     CDS             complement(<1..1015)
                     /gene="maeB"
                     /locus_tag="OO249_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5638131.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATA"
     gene            1304..2254
                     /gene="tal"
                     /locus_tag="OO249_004042"
     CDS             1304..2254
                     /gene="tal"
                     /locus_tag="OO249_004042"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5638132.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            2274..>3362
                     /locus_tag="OO249_004043"
     CDS             2274..>3362
                     /locus_tag="OO249_004043"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5638133.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTGYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNT"
BASE COUNT          841 a          862 c          870 g          789 t
ORIGIN      
        1 tggcggttgc gccaacgtcc agcgcgccac ggaagatgaa cgggaagcac aggacgttgt
       61 tcacctggtt cggatagtca gaacgaccgg tgcaaatgat ggcatccgga cgcacttctt
      121 tcgccagcgg cggcagaatt tccggttccg ggttcgccag cgccaggatc attggcgcac
      181 gagccatttt cttcaccatt tcctgggtca gcactttcgg gccggaacat ccgaggaaaa
      241 tatccgcgcc ttcaatcaca tcatcgaggg tacgtttgcc gtcgtccacc accgcatatg
      301 cggctttggt ttccgccatg tttggctcac ggccctgata gataacgcct tttgaatcgc
      361 aaaccacgat gttatgtttt tgcagaccca gcgctaccag caggttcata caggcgattg
      421 ctgcggcacc cgcgccggaa accaccatcc gcacgtcgga gatgtttttc tccaccacgc
      481 gcaagccgtt gaggatggcg gcagtgctga tgattgccgt gccgtgctga tcgtcgtgga
      541 ataccggaat attcatccgc tcgcgcagtt tctgttcaat atagaaacat tctggcgctt
      601 taatgtcttc gaggttgatt ccgccgaagg tcggttcgag cgcagcgaca acttcaataa
      661 atttgtccgg gtcgagttca tcaacttcaa tgtcaaatac atcaatcccg gcgaatttct
      721 taaacagaac gcccttgcct tccatcaccg gtttgccagc cagcgcgcca atgttgccta
      781 accccagcac cgccgtaccg ttagagatca ccgccaccag gttaccgcgc gcggtatatt
      841 tgtaggcttt taacgggtct ttttcgattt caagacaagg tgcggcaacg cctggtgagt
      901 aggccagcgc cagatcgcgc tgcgttgcca gaggcttggt tggagaaacc tggattttcc
      961 ctggaactgg aaattcatgg aaatcaagtg cactttgttt taactggtca tccatttggt
     1021 tgttcctttc acgtaacgtt cacaaataaa gtgtgtgggc aacagcccct gcccacaacg
     1081 tggcgcacat tattaccccg ccggagtcta cagactttga gcaagtccaa actctcacca
     1141 ttaatataat gttttggtaa taatcctata acactgatgt tacctgctta atccagcaat
     1201 accatgcctg tctgctatgc ttttttgatg cgtttagcga aatttctcag aagtgtgaat
     1261 taacgcactc atctaacact ttacttttca aggagtattt cctatgaacg agttagacgg
     1321 catcaaacag ttcaccactg tcgtggcaga cagcggcgat attgagtcca ttcgccatta
     1381 tcatccccag gatgccacca ccaatccttc gctgttgctc aaggctgccg gattatcaca
     1441 atatgagcat ttaatagacg atgctatcgc ctggggtaaa aaaaatggca agacccagga
     1501 acaacaggtg gtcgcagcgt gtgacaaact ggcggtcaat ttcggtgctg aaattctcaa
     1561 aatcgtaccc ggtcgcgtgt caacagaagt tgatgcacgc ctctcttttg ataaagaaaa
     1621 gagtattgag aaggcgcgcc atctggtgga cttgtatcag caacaaggcg ttgagaaatc
     1681 acgcattctg atcaaactgg cttcgacctg ggaaggaatt agcgcggcag aagagctgga
     1741 aaaagaaggt attaactgca acctgacgct gctgttttct tttgcccagg cgcgggcctg
     1801 tgcggaagca ggcgtttttc tgatttcgcc gtttgtcggg cgtatttatg actggtatca
     1861 ggcacgcaag ccgatggacc cgtatgtggt ggaagaagat ccgggcgtta aatcggtgcg
     1921 caatatctac gactactata agcaacacca ctatgaaacc attgtgatgg gcgcgagctt
     1981 ccgtcgcacc gaacaaatcc tcgccttaac cggctgcgat cgactgacta tcgcaccgaa
     2041 tttgctgaag gagctgcagg aaaaagtttc gccagtggta cgtaaattaa tcccaccttc
     2101 tcagacgttc ccacgcccag ctcccatgag cgaagcggag ttccgttggg agcacaatca
     2161 ggatgcgatg gcggtagaaa aactgtctga aggcattcgt ctgttcgccg ttgatcaacg
     2221 caaactggaa gatcttcttg ccgccaaact ataaaccagc cacggagtgt tatatgtccc
     2281 gaaaagacct tgccaatgcg attcgcgcac tcagtatgga tgcggtacaa aaagccaact
     2341 ctggtcatcc cggcgcgccg atgggcatgg ctgatattgc cgaagtgctg tggaacgatt
     2401 ttcttaaaca taaccctacc gacccaaccg ggtatgatcg cgaccgcttt attctttcca
     2461 acggtcacgc gtcgatgctg ctctacagtt tgctgcatct gaccggttac gacctgccgc
     2521 tggaagagct gaagaacttc cgtcagttgc attcgaaaac cccaggccac ccggagattg
     2581 gttatacgcc cggtgttgaa accaccaccg gtccacttgg acaaggtttg gcgaacgccg
     2641 tcggactggc gatagcagag cgtacactgg cggcgcagtt taatcagccg gatcatgaaa
     2701 ttgtcgatca cttcacctat gtgtttatgg gcgacggctg cctgatggaa ggtatttccc
     2761 acgaagtctg ttcgctggcg ggcacgctag gactgggcaa gctgattggt ttttacgatc
     2821 acaacggtat ttcgattgat ggagaaaccg aaggctggtt taccgacgat acggcaaaac
     2881 gttttgaagc ctatcactgg catgtgatcc atgaaatcga cggtcacgat ccgcaggcgg
     2941 tgaaggaagc gatccttgaa gcgcaaagcg tgaaagataa gccgtcgctg attatctgcc
     3001 gtacggtgat tggctttggt tcgccgaata aagcaggtaa ggaagaggcg cacggcgcac
     3061 cactggggga agaagaagtg gcgctggcac ggcaaaaact gggctggcac catccgccat
     3121 ttgagatccc taaagagatt tatcacgcct gggatgcccg tgaaaaaggc gaaaaagcgc
     3181 agcagagctg gaatgagaag tttgccgcct ataaaaaggc tcatccgcaa ctggcagaag
     3241 agtttacccg acggatgagc ggtggtttac cgaaggactg ggagaaaacg actcagaaat
     3301 atatcaatga gttacaggca aatccggcga aaatcgctac ccgtaaggct tcgcaaaata
     3361 cg
//
LOCUS       DAJTOR010000266         3346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTOR010000266 DAJTOR010000000
VERSION     DAJTOR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.270"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1089
                     /gene="nadB"
                     /locus_tag="OO249_004044"
     CDS             <1..1089
                     /gene="nadB"
                     /locus_tag="OO249_004044"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR5638134.1"
                     /translation="VVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPDISSG
                     DGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPD
                     FDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLGID
                     LTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLECL
                     VYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIV
                     RTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRG
                     LHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(1074..1811)
                     /gene="trmN"
                     /locus_tag="OO249_004045"
     CDS             complement(1074..1811)
                     /gene="trmN"
                     /locus_tag="OO249_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5638135.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1943..3277
                     /gene="srmB"
                     /locus_tag="OO249_004046"
     CDS             1943..3277
                     /gene="srmB"
                     /locus_tag="OO249_004046"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5638136.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT          834 a          913 c          893 g          706 t
ORIGIN      
        1 gttgtaggcg cgtgggtatg gaaccgaaat aaagaaacgg tggaaacctg ccacgcaaaa
       61 gcagtggtac tggcaaccgg cggtgcgtcg aaggtgtatc agtacaccac caatccggat
      121 atttcttctg gcgatggcat tgctatggcg tggcgcgcgg gctgtcgggt tgccaatctc
      181 gaatttaatc agttccaccc taccgcactg tatcacccac aggcacgcaa tttcctgtta
      241 acggaagcac tgcgcggcga aggcgcttat ctcaagcgcc cggatggcac gcgttttatg
      301 cccgattttg atgagcgcgg cgaactggcc ccgcgcgata ttgtcgcccg cgccattgac
      361 catgaaatga aacgcctcgg cgcagattgt atgttcctcg acatcagcca taagcccgcc
      421 gattttattc gccagcattt cccgatgatt tatgaaaaat tgctcgggct ggggattgat
      481 ctcacacaag aaccggtacc gattgtgcct gctgcacact atacctgcgg tggtgtaatg
      541 gttgatgatc atgggcgtac ggacgtcgag ggcttgtatg ccattggcga ggtgagttat
      601 accggcttac acggcgctaa ccgcatggcc tcgaattcat tgctggagtg tctggtctat
      661 ggctggtcgg cggcagaaga tatcaccaga cgtatgcctg atgcccacgg cgtcagtacg
      721 ttaccaccgt gggatgaaag ccgcgttgag aaccctgacg aacgggtagt aattcagcat
      781 aactggcacg agctacggct gtttatgtgg gattacgtcg gcattgtgcg cacaacaaaa
      841 cgtcttgagc gcgccctgcg gcgtatcaat atgttgcaac aggaaataga cgaatattac
      901 gcccacttcc gcgtgtcgaa taatttgctg gagctgcgta atctggtgca ggttgcggaa
      961 ttgattgttc gctgtgccat gatgcgcaag gagagtcgtg gcctgcattt cacactggat
     1021 tatccggaac tgctcaccca ttccggtccg tcgatccttt cccccggcaa tcattacata
     1081 aacagataaa aagcctgggt cagcgccgta tacgcttcgg aatagttctg gtctggccca
     1141 cgaatgacta agcgatcgct aaagcattct cccgcctgcg gggagaatgc cagcagcacc
     1201 cgatgcggca gtcgcgcttc gttttccgcc acatccgtcc gcaaacgtaa atgccagccc
     1261 atgcttaatg ccagctccgt aaaaccatta ccaatctgct ctggcagcac tacgcagaaa
     1321 aatccctctt cggtaatgca ctccgccgca caggtcagca gcgatgggtg atcaagcgta
     1381 gtggtatagc gagcctgttc ccgttgaggg atcgcgcact ctactccctg ctgatagtaa
     1441 ggtgggttac tgatgattaa atcgaagcgt actgtctgct gtgtgagcca ctgctgaata
     1501 tccgccgtat ggacgttaat ccgctctgcc cacggggact ggttgatatt ttcctgcgcc
     1561 tgcgcggcag cttcactttc cagctcaaca gcatcaatta tcacgctgtc actggttcgc
     1621 tgcgccagca tcagtgccag caacccgcta cccgcgccga tatcaaggca acgtttcacc
     1681 ccagccaccg gtgcccatgc gcccagcaaa atgccatccg ttcccacttt catcgcacag
     1741 cgatcgtgag caacaaaaaa ctgtttaaaa gtaaatccat tacgacgaag cacggatgta
     1801 gactgtgaca tgaaaataaa accttgcagg aaaaacggcg atagcaccgg gtgagaacaa
     1861 tacccgagaa gcgatatcca tacaaacaga tgaagattgc agccgtaacg tctataatca
     1921 gcgccccaca cagaggtaga acatgactgt aacgactttt tccgaacttg aactcgacga
     1981 aagcctgctg gaagccctcc aggataaagg tttcactcgc ccgaccgcca ttcaggctgc
     2041 cgccattccg cctgcgctcg atggccgtga tgtactcggt tctgcgccga caggcaccgg
     2101 taaaacggcg gcgtatctgc tgccagcgtt gcagcacctg ctcgatttcc cgcgtaagaa
     2161 atccggtccg ccgcgtattt tgatcctcac cccaactcgc gagctggcga tgcaggtggc
     2221 cgatcatgcc cgcgaactgg cgaaacatac gcatctggat atcgccacca ttaccggcgg
     2281 cgtagcctat atgaaccacg cggaagtgtt cagcgaaaat caggacatcg tggtcgccac
     2341 gaccggacgt ctgctgcaat acataaaaga agagaacttc gattgccgcg cggttgaaac
     2401 gctgatcctc gacgaagcag accgtatgct ggatatgggc ttcgctcagg atatcgaaca
     2461 tattgctggc gaaacgcgct ggcgtaaaca gaccctgctc ttttcggcaa cgctggaagg
     2521 cgatgcgatt caggactttg ccgagcgtct gctggaagat ccggtggagg tttctgccaa
     2581 tccgtccacc cgtgagcgca aaaaaattca tcagtggtat taccgcgccg atgacctgga
     2641 acataaaacc gcattgctgg tgcatctgtt aaaacagccg gaagcgaccc gctcaattgt
     2701 gtttgtgcgt aagcgtgaac gtgtgcatga gctggcaaac tggctgcgcg aagcgggcat
     2761 caacaactgc tatctcgaag gtgagatggt acagggcaag cgtaatgaag cgattaaacg
     2821 cctgaccgaa ggccgcgtaa acgtactggt agcaaccgat gttgccgcgc gcggtatcga
     2881 cattccagac gtcagccacg tctttaactt cgatatgccg cgcagtggtg atacttattt
     2941 gcaccgtatc ggacgtaccg cgcgcgccgg tcgtaaaggc accgcgattt cgctggtgga
     3001 agcccatgac catctgctgc ttggcaaagt tggccgttat attgaagagc cgataaaagc
     3061 tcgcgtcatt gaggaactac gcccgaaaac gcgtacgcca agcgagaagc agaccggcaa
     3121 gccatcgaag aaagtactgg caaaacgcgc cgagaagaaa aaagctaaag agaaagagaa
     3181 gccgcgggtg aaaaaacgcc atcgcgacac caaaaatatt ggtaagcgcc gtaaaccgag
     3241 cggaacgggc gtgccaccgc aaacgacaga agagtaatct taattccggg tttaagcccg
     3301 gaattaaaag tgctggcaaa cgcaaaactg cctgatgcgc tacgct
//
LOCUS       DAJTOR010000267         3325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTOR010000267 DAJTOR010000000
VERSION     DAJTOR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.271"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..837
                     /gene="tauA"
                     /locus_tag="OO249_004047"
     CDS             <1..837
                     /gene="tauA"
                     /locus_tag="OO249_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5638137.1"
                     /translation="NTFAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVA
                     ASQQVPIEVFLLASKLGNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHW
                     GIKLGQVEIVNLQPPAIIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAP
                     TLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPE
                     GDVPGLVKGNTYLTPQQQTAELTGPVNKVIIDTAQFLKEQGKVPAVANDYSQYVTSRF
                     VQ"
     gene            850..1617
                     /gene="tauB"
                     /locus_tag="OO249_004048"
     CDS             850..1617
                     /gene="tauB"
                     /locus_tag="OO249_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5638138.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1614..2423
                     /gene="tauC"
                     /locus_tag="OO249_004049"
     CDS             1614..2423
                     /gene="tauC"
                     /locus_tag="OO249_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5638139.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTP"
     gene            2438..3289
                     /gene="tauD"
                     /locus_tag="OO249_004050"
     CDS             2438..3289
                     /gene="tauD"
                     /locus_tag="OO249_004050"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5638140.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGITAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRVTILGD
                     KPFYRAG"
BASE COUNT          709 a          850 c         1028 g          738 t
ORIGIN      
        1 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
       61 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      121 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      181 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggc
      241 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      301 cactggggga ttaaactagg gcaagtggag attgtgaacc tgcagccgcc cgcgattatc
      361 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
      421 ctggaaaaag acggcaaggt gttgaccgat tctgaacagg tcgggcagtg gggtgcgcca
      481 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
      541 gcgttcgcta aaagcgccat cgatgctcag caaccgtaca ttgctaaccc agacgcgtgg
      601 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc ggaaggtgac
      661 gttccggggc tggttaaggg gaatacctat ctgacgccgc agcaacaaac ggcagaactg
      721 accggaccgg tgaacaaagt aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
      781 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
      841 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
      901 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
      961 tgcggtaaaa ccacgttgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1021 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgcggggt ggtttttcag
     1081 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1141 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc aggtgctgaa aaaagtgggg
     1201 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg gtggtcaacg tcagcgggtg
     1261 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1321 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1381 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     1441 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcc gctcaacttt
     1501 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     1561 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     1621 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     1681 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     1741 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     1801 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     1861 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     1921 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     1981 gtccggtgtc gccgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2041 cctcgaagat cttactgatc tatttagcga tttttgcacc ggtggcgatg tcggcgctgg
     2101 cgggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2161 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2221 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2281 cgcgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtgc
     2341 tggcgggaat cgcggtgatt gcgattatcg cctttctttt agaactgggt ttgcgcgcgt
     2401 tacagcgccg tctgacgccc tagcatggag aagtacaatg agtgaacgtc tgagcattac
     2461 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     2521 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tgtttctgcg
     2581 cgatcaagcc attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     2641 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     2701 taacgataat ccgccagata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     2761 acccgcaggg gcgattctgg cagctaaaga attaccttcg accggcggtg atacgctctg
     2821 gaccagcggt attacggcct atgaggcgct ctctgttccc ttccgccagc tgctgagcgg
     2881 gctgcgtgcg gagcatgatt tccgtaaatc gttcccggaa tacaaatacc gcaaaaccga
     2941 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3001 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3061 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3121 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3181 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3241 gcatcgggtg acgattcttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3301 acggttccct cgcccccttg gggag
//
LOCUS       DAJTOR010000268         3224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTOR010000268 DAJTOR010000000
VERSION     DAJTOR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.273"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..222)
                     /locus_tag="OO249_004051"
     CDS             complement(<1..222)
                     /locus_tag="OO249_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5638141.1"
                     /translation="MKACLLLFFYFSLIDQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNI"
     gene            complement(234..>3224)
                     /locus_tag="OO249_004052"
     CDS             complement(234..>3224)
                     /locus_tag="OO249_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5638142.1"
                     /translation="EHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRIT
                     TPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIIRYRYDNPHS
                     DLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYR
                     AYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRTTQGEL
                     TRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSE
                     DEGLVTHWHYDEADRLTHRTVNGETAEQWQYDKRGWLTDISHLSEGHRVTVHYRYDEK
                     GRLTGERQTVHHPQPEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGM
                     KLGDTPLVDFTRDRLHRETLRRFGRYELSTAYTPAGQLQSQHLNSLQYDRDYTWNDNG
                     ELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTL
                     SMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGGIRTDDERTHRYHYDSQHRLVHYT
                     RTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTI
                     QNDRSRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADTLQQSGGEDGGSVVFPPVL
                     VQMLDRLESEILADRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIHLYHCDHRGL
                     PLALISTEGATAWCAEYDEWSNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYD
                     PLQGRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDLIHSVADEINIP
                     GVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNTSKGQNSFA
                     SQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMAITTKDLGNW
                     ITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          604 a          907 c          873 g          840 t
ORIGIN      
        1 aatattattt ttagtaaagc agtaatattg atatagtcga aatgatgcct ctgaatcacc
       61 atgttgtgat ttatacttca gtttcatcag cttttcttca cttaaatcat aggtcattgc
      121 tgtagatccc atcacacttt cgttttcttt tattttcaca tcagcaccat gcaattgatc
      181 aataagagaa aaataaaaaa atagtaacaa gcatgctttc atacatctac aacctatctt
      241 atatcactgg gtttttctgg cgtaccttgc atattagaaa tggggtgccc agatggccca
      301 tatgttatcc aattaccaag gtcttttgtt gttatagcca ttagatcctt atttgaatta
      361 aggttcgttg gtgctgttag cactgtatcc atttttaact taccattagt tcctcgcccc
      421 caccacgtcc atagcgtgtc aggtcctatt acatttgtat gtaactcttt agctaattgg
      481 ctagcaaatg aattttgtcc tttacttgta ttacaagaaa ataaccaaac tgtcatccca
      541 tctttataat tcccatcaaa tttaattaga tatgctagat ctttagctgt catgatactg
      601 cgcgttgcgg attcaataga tgtgggggta ccatgccccc cgattgtgaa aacgcctggg
      661 atattaatct catcagctac agaatggata agatcacttt cggggtataa atttatatca
      721 accaaacctt gcggatctac atttatgacc ggattcaacg gatactgata aaaattccat
      781 ccccccttca gcccaatcgg atcctgagtg atataccgcc cctgcagcgg gtcataatag
      841 cggtggcggt tgtaatacag gccggactcc tcatcatact gctgacccgg caggcgaatg
      901 agctgctgca gatgatgcgg gttctcatca ctcagcaggt tgctccattc atcatattct
      961 gcgcaccacg ctgttgcccc ttccgtgctg atgagcgcca gcggcaggcc gcgatggtcg
     1021 cagtggtaca ggtggatttt tcgcgccggc gtgtacaccg ggtccatctg gctttgcatc
     1081 tgcgccaccg tcaggccgca cgatgccagc cagcggcggc tttcctcact cacccggtca
     1141 gccaggattt cactttccag ccggtcgagc atctgcacca gcaccggcgg gaacaccaca
     1201 ctgccaccgt cttcgccgcc ggactgctga agggtatccg ccaggctgcg gcgctgcgtt
     1261 ttcgccagct caccggtggc ggtttcaact ctgatgagtg gcgtgaagct ccccggctga
     1321 taaatcgtct ggatgcggct cctgtcgttc tgtattgtgg tcagccggtc gccgtcccag
     1381 ccgtaccagg tcacttccgg tttccgtgac agcgacatcc agcccgtcag gtcacgttca
     1441 cgccgccaca cccgttttgc caccctgcgg cccagcgggt cgtaaagata gcgactttcg
     1501 accagcggct ctgcatattg tgtccgcgtg tagtgcacca gccggtgctg actgtcgtaa
     1561 tggtaccggt gggtgcgctc atcatccgtg cggatacccc cttccgggat gaggtcggtt
     1621 ttctctgtca gcctgccgtg acggtcatac cggtaaagat agtgcgcgtc acgggcgata
     1681 cggttatccg gccacatgct gagggtgctg tccgggtgca gctccgggtc gggcaggcgg
     1741 ttacctgccg ggtctgtggc atacgggatg cggatatcaa ggttcgctgc ggtggtgtga
     1801 acgccggtca gcctgccggt ggtgctgtag ctgtaactcc gggtctggcg cgggctgctg
     1861 atgcggatga gttcgccgtt gtcgttccag gtgtaatcgc ggtcatactg caggctgttc
     1921 aggtgctggc tctgtaactg cccggcaggg gtgtaagcgg tggagagttc ataacggccg
     1981 aagcggcgca gcgtttcccg gtgcaggcgg tcgcgggtga aatccaccag cggtgtgtcg
     2041 ccgagcttca tacctgccag ccagccgctg ccgtaggtca gccattccac ggcgggcagg
     2101 ctgtccggta tacagcggtt cgccagtccc tgcgcgttgt acgcatgtct ggtctcatgc
     2161 tgccagagca gtgcttccgg ctgcgggtga tgcaccgtct ggcgctcacc ggtcagccgg
     2221 cctttctcat catacctgta atgcaccgtc acccggtgcc cttcgctgag atggctgatg
     2281 tctgtcagcc agcctcgttt gtcatactgc cactgctctg cggtttcgcc attcacggtg
     2341 cggtgcgtga ggcggtctgc ttcgtcatag tgccagtggg tgaccagccc ctcatcctcg
     2401 ctgcggataa gtttgccggt caggtcgtgg tgataacgct gtgtgcggcc gtcaaagccg
     2461 gtttcctgta tcagccggtc gagtacatcg taacggaagg tggtgtggct gccgttttca
     2521 ctggtcaggc ggatgacccg tcctgcagca tcgtattcca tactgcgcgt cagctcgccc
     2581 tgcgtggtac ggatggcctt tccccacgca tcgtactgtg tcccgtttct gctgccgtcc
     2641 ggggcaatga cggcggtcag gtcaccggcg gcgttgtatt cataccgcgt ttcatggccc
     2701 tgcgtgtctt tcacggcaat taactgtcca cggctgtcgt atgcgcggta ctgactcagc
     2761 ccttcctcgc ggtgcaccgc cgtcatctgc ccgaagcggt catggtcata acgggttaca
     2821 taaccggaac agtcggtgaa gctcagcaac tgaccgtaac ggctccacgt catggttttc
     2881 cggctaccgg tggcatcttc cgttgcgcag ggtaagtcac tgtgtggatt atcataacgg
     2941 tagcggatga tatcgccgtc aggggcagtt tcctgaatca gacggcccca ttcatcatat
     3001 tcccggcgta tttccagccc gtcaggcccg gtggctgacg ttaactggct gtggtggtta
     3061 tagtaaaacg ccgatgccct gccatccggc gtggtgatgc gcgtgatgag gcccgtcacc
     3121 acatccgggc tgtattctgt tgtccggcct gcggcatccg tctgtgtcct gagcctgccc
     3181 acggcgtcaa actgactctg cgtgacgctg ccgtccgcgt gttc
//
LOCUS       DAJTOR010000269         3217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTOR010000269 DAJTOR010000000
VERSION     DAJTOR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.274"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..1010)
                     /locus_tag="OO249_004053"
     CDS             complement(<1..1010)
                     /locus_tag="OO249_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5638143.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSKNYGCSLDNTDVNWCSVVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETIINPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GYLGIMTEGENFLCRLLTIEDEVLNVLLRENFNEQLDTNINTIISNLNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NG"
     gene            complement(1272..2862)
                     /locus_tag="OO249_004054"
                     /pseudo
     CDS             complement(1272..2862)
                     /locus_tag="OO249_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT         1031 a          591 c          503 g         1092 t
ORIGIN      
        1 ttaccattgc aattcgtttc atgatttcca gctaatacat gaacattttc attgatactc
       61 attatgcgtt ttaacaaatc gatgataaat tgatcgccac agatattact aaatcgatcg
      121 ccggtttggt cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aactaaataa
      181 ggctgaagac tttctaaaac attatctctt ctgttcaggt tactgattat tgtattaata
      241 ttagtgtcaa gttgctcatt aaagttttcc cttagcaata cgttgagaac ttcatcttct
      301 atagtaagca accggcaaag gaaattttct ccttcagtca tgattcccaa atatcccgta
      361 gcaaaaagcg cgtacaaaac tgcaccaact gaaccatcgg tatccccaaa ataggtcggc
      421 ctggtacttg tgggatcaac aggtgaacaa ttggagaaat agattctgtc agcgaactgg
      481 gggtttataa ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc
      541 cagatgggtt tgtacgactc agtaactgca acatcattag cattatcatt tactgtcata
      601 ttattaagta aataaacaac actgcaccaa ttaacatcag tattatcaag agagcaaccg
      661 taatttttac tattaattgt cacaataaac tgataattat tatccctacg tagaatatga
      721 gcacgcatgc ccgatagcat actatatttt tctgaatgat ccgtttgtat ggtaaatgaa
      781 attttgtcat cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc
      841 acaacctcac cgagcattgt cctggcaata cttgatatat catcacctat accatttgca
      901 atatacccaa acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt
      961 gcgattgtcg ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc
     1021 atattattca ccgcgaggaa tacatagtta acattttatc ttatgacata cacctcatgt
     1081 tcgatggaaa aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat
     1141 ttcttatttc atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgtct
     1201 ctacaatcga ttaagaaaac ctatgaaata ctggcaggat taaaaagata taccacagca
     1261 ctcatggagt tttaatgtgg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca
     1321 cgttcgttag gagctatctt ccaggaattt agccccatgg tataattatc aattttttga
     1381 tgctgatcta cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt
     1441 ccacatttga agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattg
     1501 tcatatacag gtgtggatgc tatagcattg cttctaatta gatggataaa acgatcatcc
     1561 atgtgttgtt tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct
     1621 ttcatctgat taaatggaac ctgcaaagca cctagacaat aacattcatt ttcttcattt
     1681 cttattattc cgtgatgact gcttaaaact ttcgtttctg agtcataatt acaaacatcg
     1741 agcgttttaa ttaagttata cgtatcaccg gcagagagtt tatgatttgc tagtcgtgca
     1801 gtatagttac cattgaaatt gatttcataa tttccagcca gaacgacgtc atttttattt
     1861 atacgactat ctttattact ctccatattc cgcatagaat ttagcaaagt aaggatatat
     1921 ttatcaccaa aaatagtact gaatctatcg cctgtatgat ccccaagaaa gatacaaggg
     1981 gtaatcgcat cgccttttaa aaaaacatgt tgatgcaact tattcaagat catgtctctc
     2041 gtatctaata aagtaaagag tttttccaat tttttatttt catatgcgaa tgaagccatg
     2101 tcttcgtgtt ttaataattc acataataaa ctccacccct tttccctgat acctatatgt
     2161 cctgaaacga gcaatgcata aagtacagcg ccaactcttc catctgtatc gccaaaataa
     2221 gccgtgttgg gattttgtgg gttaatcaca ccacactgtt tataaaaatc tatttttgaa
     2281 aacacagaat taacatagct agcaaactta tccttaggaa acgtctcttt agagacatcc
     2341 caacgctagt cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata
     2401 gaaaggccat taactttata aattatgtct gatttatctc cggagaaact aataaaataa
     2461 taaccatcct tacactcaat cgtaacggta atgggagacg acaattttat tacatggtta
     2521 ttgtctggta taattgtaaa gataaacgtg tcagtagtat tatcggcgaa actattatat
     2581 tcttgaatat ctatagatgg ctcattattg taaaaccata tttttttcta tctgagaact
     2641 atattcattt accttccagc gaccatgttg caggaaatag atgatatgca ggcctaaatc
     2701 gttatctatt tgcgaatgtt tatccagctt atcaatttca actatctttg aacgatgttt
     2761 tttatcaacc ttgccatggt gttgatgatt gttaacttga tctgaatgct tatcttgatg
     2821 gcttgaagag atgtgcttaa atatattgtg aaatactttc ataaataacc cgcaatattt
     2881 ataacttgtt agtaacaaaa taaacgatac atgtctgaaa gacatattta cgacgataca
     2941 aagtaataat acttatatat ccgatgattt taagcaccga gactatccgg tcttttacta
     3001 aataatggaa aacaatccat ccctggaaga taattgaaaa aaacaacaga ataagaatta
     3061 atcttaggat aaacatttat ttatcatggc cttttgaacg ctgaaaagta gcgctctgta
     3121 aagtaaaccc cgctatagtt atgctatgca tttgtcttta atgatatctc gtcattgtat
     3181 taacaggctg aaattatcat taatgcacat ttcaata
//
LOCUS       DAJTOR010000270         3207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTOR010000270 DAJTOR010000000
VERSION     DAJTOR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.275"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            227..599
                     /locus_tag="OO249_004055"
                     /pseudo
     CDS             227..599
                     /locus_tag="OO249_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1161..2108)
                     /gene="icsP"
                     /locus_tag="OO249_004056"
     CDS             complement(1161..2108)
                     /gene="icsP"
                     /locus_tag="OO249_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5638144.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGTHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT          948 a          658 c          624 g          977 t
ORIGIN      
        1 gcgatctggt tcaacaatat gcagatggta cacaacctct ttaaccagcc catccggatt
       61 attcagagcc cttccgcaat ccggaataaa agtgctgttt taccgtgaac cctctgaaaa
      121 tcagcgagca gtgttttaac cagccgacgt tcagagcgta ctgacagacg gtggacaatt
      181 tgaataaaca gttcaatcag accgtagatg ctctcccgtt ggcgacatgc tgggagcggt
      241 ggaacaccgc ttcggcaacg agcttccggc gtctccagtg aagtgactga cggataatgg
      301 ttcatgctac cgggctaatg aaacacgcca gttcacccgg atgttgggac tttgttgaac
      361 cgaagaacac ggcggtgcgg agtccggaga gtaacagaat agcaaagagc ttcgtgaaaa
      421 caataaagtg tgactacatc agtatcatgc ccaaacctga cgggttaacg gcagcaaaga
      481 accttgcaga ggcattcgag cattataacg aatggcatcc gcatagtgcg ctggattatc
      541 gctcgccacg ggaatatctg cggcagcggg ccaatgataa caggtgtctg gaaatatagg
      601 ggcaaagcca cccacgaacg atccagcata taatcaagaa attagatttc atttaacata
      661 agatagatta tgtgcaccaa gaggcatttc cttaatttct gaatttacag gctcttttgc
      721 tctctttgct atgctgtgga tttggaaaga tagagtgctg gctgattatt taggtatttt
      781 catcagcatg tccctgatag cactgttcca tcagcgcttt caggcgataa tagtagacct
      841 tgggtagcct ctgctcatgt gtaacttgat accccgagaa cagccacgat gatctaagcg
      901 gttcttattt ctccggtctc agggtcggta atgccaacag taagtccaca gtaatcaacg
      961 aagagttttt tgaaccgccc cggaaatcct ggagactaaa cgccctgaga aagaggtaaa
     1021 cagaatgact aaaaatacac gtttttcccc cgaagtccgt caacgggcag ttcgtatggt
     1081 tctggaaagt cagggcgaat atgactcaca atgggcggca atttttagcc atgaaaacgt
     1141 aatcaactcg gtgaaccaat tcaaaaaata tactttatac ctgcggaagt gagaaaacca
     1201 atatattcaa tacttgcact atttttaatg gtgccagata tattggtact ttgctcataa
     1261 agagatgtat cacctttttt attagaaatg taatttctgg agccttctat ataaaatttt
     1321 gcactggggg tgacatagta tccaatattc gcagcaacag agcagtaatt ttggtttttt
     1381 atttcatctt taaaaattgt ctcagtctga tagtgtttat ctgtatcaga tgaacgaacc
     1441 caaccactat atttcagttc tgctccaaac tcaaaattct catggcggta attagcagtt
     1501 aatccaatat aaggtatttt gaagagctgt ttgtaaccta ttgttctttc accactagga
     1561 tgtgtccctt ttttattcct gttccccccg ttttcactat aaatataact ccctcccatt
     1621 gcattaaaac tgtaacgact ctcttggtaa cctgctatta gtccaagtcg ataatccaaa
     1681 ttatttaata accaaccttt aagattcagc tcgaattcgt tagcatcacg gagatgcgta
     1741 ttgggatgcc agctctggtc ggtccatacc tgaggggtat tggaattgtt ccagtcttta
     1801 tcaaccatgc ttgctttctg gttacctaaa gtagtccaac cggaaactcc gaaagacact
     1861 tttggaatta gcttccaatc gatgccacct ctaacaatag tcgcattact gtatttccag
     1921 tccagttgac taatttttct ccccccttcc ttgggatgat aaacgcgttc ttttgttttg
     1981 ccactcagag atcctagact aatatccgta ctgatgttat ccggtatgaa aagtggatag
     2041 ttagtggtag catgtgtagt aaagatcgca ggtacacaaa gtgcaagtac aaggaatttt
     2101 aatttcatcg agaactctac ttttttggtt gaaatgtcca tgatcttctt actttataag
     2161 ataaacgtca atgccaagca ttattttaat taaatatttg tttatgttac caagaacata
     2221 tcttttatta taatatttat tgaacttttg taaaaataaa aaaatacctc attgtgcgaa
     2281 taaagtaacg ggggccatag atttctctac ctaccttttg gtgttcatac ctgaggctga
     2341 tagtttcagg attccgcgac aattctactc accttcttgt tctctcgcca atatgaaatc
     2401 aatcagaaaa gcgttccata agaacaacga taagacaata aattgtcatc acggctgcaa
     2461 tagccaacgt gcctttcagt accaacatga caacctcctg tatttgacgt aacgtgtgct
     2521 gataaatatg tatttgtatc tcaactgact caatacaaca cttaagtata gtttttcaga
     2581 ttttgttttt cccgtgaaat attatggaaa tggcagaagc aatacattga attccgattc
     2641 gagtgaatac ggctagcggg cacctcactt tagcactgaa gccatcaact tttatttgac
     2701 ttatttcaat ggaaagcttt ctcactatca gtacaggtaa ttcccatcct tttaaagtcg
     2761 agagtcagac tgcgactaat acagttaaag tgaccaaact cagttatcct ctacacattt
     2821 tacctcatgt ttacttggac aattcattgg tgcttttata aaagaataaa tattctacta
     2881 atttaatgca tcacctaaaa tagtattttt tataatagaa ctgtatctat agtatattat
     2941 taaagcttaa ttatttatta tgctttaatg tggttgtccg attaaggact aatccttgaa
     3001 aacgccgtat agggcgggag ttcactctat tgcgataacg taaccgtaca atattgtgcg
     3061 tttcccaaat tgaccactac tgaactattc tgggatttaa atgcaataaa atggttggtt
     3121 gaaggtcgtg gtgatgatgt tagtcctttt gagaatattc cagtgccaga tcttacttat
     3181 aataaagagg cttggcagtt tggataa
//
LOCUS       DAJTOR010000271         3196 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTOR010000271 DAJTOR010000000
VERSION     DAJTOR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.276"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            577..1293
                     /locus_tag="OO249_004057"
     CDS             577..1293
                     /locus_tag="OO249_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638145.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1845..2249
                     /locus_tag="OO249_004058"
     CDS             1845..2249
                     /locus_tag="OO249_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638146.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2312..2946
                     /locus_tag="OO249_004059"
                     /pseudo
     CDS             2312..2946
                     /locus_tag="OO249_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2988..>3196)
                     /locus_tag="OO249_004060"
     CDS             complement(2988..>3196)
                     /locus_tag="OO249_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5638147.1"
                     /translation="CYDNAPMESFWGTLKNESLSHYRFKSRDEAISVIREYIEIFYNR
                     QRRHSRLGNISPAAFRIKYYQMTA"
BASE COUNT          885 a          665 c          725 g          921 t
ORIGIN      
        1 ttgtgggcca taaatattcc aaaaaaatag cccgctccgt ttattaccgg aacgggcgag
       61 agcatgacat tgtctgtaat tataatgaga ctgctgattc tacttatttt taatttcaag
      121 aaaagcgttt tctacaaaga attatgagat ttaatgtttt gtttagacaa tattgtcatt
      181 ctgttcaata atgaaagtct tcatcaacaa caagtaaaaa caacgtccct ctcatcgaac
      241 cggacttgca gatttcccgc atccggctct cttgcaacac atcagacctt cacgttcggg
      301 cggttatggg gaaggtccag caagctgtat gagccctaac tgtttgaata ccagtagatt
      361 accagaaact tctatcgatt gaagacatta ccaactagcg ttactaaatg ttgaccaagt
      421 tttgttcact tgtctggttt tgatagtagc attctgcaga gtattttgat atgataaata
      481 tgcattaaca atgatattat tgttaatttc ctagcatgaa aattaaaaga tcaacttttt
      541 atctcgaata ttttttacat tatcagttgg tttcttatga atgacaattc attgcttaga
      601 aattcttcat tattcatcgc ctatatggga tgtgtagggt gggtcagtgc ttattcttat
      661 ggctggggaa cctcttttta ctatggattt ccatggtggg ttgttggtgc aggtttggat
      721 gatgttgcca gaagtttact ctatgctatc atagtaatgg gtatactttt taccggttgg
      781 gggattggta ttttattttt tcttcttata aagaaaagaa gtaaaataca agacttgagt
      841 ttttttagac tattttttgc tattacccta ctgtttttcc ctgtaatatt cgaactttta
      901 attctcaaac aatatttcat acttccatta tcactttcat gcattattag ttcattggtt
      961 atttcaataa taattcgcat atatggacgg atcttttctg tatcatgttt ttcagatatc
     1021 ccttttgtta gagaacaccg cattaagtta attatggctg gttttttggt ttatttttgg
     1081 cttttttcct ttcttgttgg ctggtataaa cctcaattaa aaaaagaata tcagatgctt
     1141 tgttataata atagttggta ttatgttctt gctcgttatg attcacggct cgttttgtct
     1201 agttcattta aagatgacag taacagattc ttaatattta atacagaaca gtcgggattt
     1261 tatgaaatca atgatgttta tgtcagaaag taataatcct ctcccacaaa gtgaattaaa
     1321 cgaatatata atgcgttcga agtatatgta ttgagctgtc atactgagaa acaaacctta
     1381 gggcagcaca attaaagcca gaaacaacta tgcaagaaag ttactaaata agtgctcgga
     1441 acagtaaaac actcaggggg gactcagtcc agattatgta atcagataaa ttgccaaaca
     1501 tcacaaatca ccagcaggac tgagtgatgc cgagcattgc accaataccc cgtgatgaac
     1561 gatgcctgat gcagaaagct ccctataaaa caaagaacaa aaactacgac cggcgattca
     1621 ccgtcatact gatgctgcat cgtggtgaca ctgtctgtca ctttcatgtt gactgaacaa
     1681 ctctgctgcg aagaaggtgt accgccggcg ggatttgatt gtccggcgtc tagcttccac
     1741 ttccctgatg gaactgaaag ttgttatggc cggtcagctt gccctgtccg caggacatga
     1801 ggtgctgtga cggacagcac cagtaacgga aagtgacaat aaaaatgaat actgcgctga
     1861 tagttgcact aatgtgtatg tggtatgcgg tacccgctgc tgcaaaagag actttactgg
     1921 ctatgccacg taattcaaca gaacattgct atgcggagat taatgtgcac ggaccatatg
     1981 gggtgtattt ccgagtggtc ccgcatcccc ccgggggaaa gagctgggtc gaatgtaaca
     2041 gtgactacta ttactctgat aaaccaccgg gagtacagat actcggaacc cgtgcagggt
     2101 gtcgggtata cggaatctgc gggaccacct cgacactgca tgtggctgga agaggggttg
     2161 tgtgtattaa aaatatatgc agtccacgtg ggatgataat tcacaggata cggaaacgtc
     2221 ctgtagtggc tgtaagcgat gagatgtgat cttatccagc aatagtggac acgcggctaa
     2281 gtgagtaaac tctcagtcag aggtgactca catgacaaaa acagtatcaa ccagcaaaaa
     2341 aacacgtaaa cagcattcgc ctgaattttg cagtgaagcc ctgaagcttg ctgaacgcat
     2401 cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct atgcgtggcg
     2461 cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg ccgctgaaaa
     2521 tgcccgcctg aagcggcaac tggcggtgca ggcagaggaa ttggctatcc tccaaaaggc
     2581 cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcaggct
     2641 gagttcagca tcaaggcaat gtgccgcgcg ctccgggtgg cccgcagcgg ctggtatacg
     2701 tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca ctgcgacagc
     2761 gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc acgcctgacg
     2821 gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc
     2881 cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat
     2941 ggcctgaggt gtactggcaa tagcggacac caccatttgt tctttttcta agcagtcatc
     3001 tgataatatt ttatcctgaa ggctgccggg gagatattcc ccagacgaga gtgacgacgc
     3061 tgacgattgt agaaaatctc aatgtattcc cgtattactg agatggcctc atcccggctc
     3121 ttaaaacgat agtggctcag gctttcattt ttcagcgttc cccagaagct ttccatcgga
     3181 gcgttgtcgt aacagt
//
LOCUS       DAJTOR010000272         3190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTOR010000272 DAJTOR010000000
VERSION     DAJTOR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.277"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            189..929
                     /locus_tag="OO249_004061"
     CDS             189..929
                     /locus_tag="OO249_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5638148.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(900..1667)
                     /locus_tag="OO249_004062"
     CDS             complement(900..1667)
                     /locus_tag="OO249_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5638149.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1873..2451)
                     /gene="lpcA"
                     /locus_tag="OO249_004063"
     CDS             complement(1873..2451)
                     /gene="lpcA"
                     /locus_tag="OO249_004063"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5638150.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2691..>3190
                     /gene="fadE"
                     /locus_tag="OO249_004064"
     CDS             2691..>3190
                     /gene="fadE"
                     /locus_tag="OO249_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="functions in fatty acid oxidation; converts
                     acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCR5638151.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFA"
BASE COUNT          762 a          810 c          819 g          799 t
ORIGIN      
        1 ataaatgata catgcgcttt tcggactatt gaaaactgcc cctgtgctaa atttgtaaac
       61 cctgcgccga caggcttgcg taaaactctt ttggtgataa tgtgagcctt ccaaatccag
      121 actattccga tttgggatcc attttgccgt cctggcgtgt gtaaccgttt tatcaaggaa
      181 taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg tgcgtttcgt
      241 ttgccggttt gctgggcagc agtagctcga ccacgccagt gagcaaagag tataagcagc
      301 agttgatggg atcccctgtc tacatccaga tcttcaagga agaacgtacg ctcgatctct
      361 acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta taaaatctgt aaatattccg
      421 gcggcttagg gccaaaacag cgtcagggcg atttcaaaag cccggaaggg ttttatagcg
      481 tccagcgtaa tcagttaaaa ccagacagcc gttactacaa agcgattaat attggtttcc
      541 ccaatgccta tgaccgtgcg catggttatg aagggaaata cctgatgatc cacggcgatt
      601 gtgtttccat cggctgctac gcaatgacca atcagggtat tgatgagatc ttccagttcg
      661 ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt gagtatttac ccgttccgca
      721 tgaccgacgc caatatgaag cgccataaat attccaactt taaggacttc tgggagcaac
      781 tgaagccggg ttacgactac tttgagcaaa cccgtaagcc accgaccgtt tctgtggtca
      841 atggtcgtta cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca caactggcat
      901 caaactacac gctccccgag gcaaaataag cgccattcgc ctggcataat cttttgccag
      961 gtttcattgc ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt cggtgtggtc
     1021 tgcgagctga agtcgatttc tacatcctga tccagcagcg ttgccacgcc aaacggtgcg
     1081 cggcgggtga tccagtgcag attcgtcgag caatacgcca ttacatagcg cccatccgaa
     1141 agcagcatgt tgaaaacgcc cttctgccgc agttcgtccg ccagtgaagc gatatattta
     1201 aacactgctg ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt atgcagcagc
     1261 cagcagaagg ctttttcgct gtcggtttcg cctaccgggc ggaagttgcc ggtttccagt
     1321 gatttgtagc ccgtcagttg tccgttatgg gcataagtcc agttacgccc ccataactcg
     1381 cgggtaaatg ggtgagtatt ttccaacgcc acctcgcccc gattagcctg gcgaatatga
     1441 gccaccaccg aacaggattt tatcgggtag tcctggacaa gtttggcgat gggggaatta
     1501 aagctgggtt gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa ggtaatgccc
     1561 cagccatctt tatgtggccc ggttccacca ccacgctgta caagtccggt gaaactaaag
     1621 cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg taacctccac
     1681 aacagcggga gcgcatggcc cccggcgaag attgatttaa gaattttcag gtcggatgcg
     1741 gcgtaaacga attattcggc ctacggttcg cgtacagatt tgtaggcatg ataagacgcg
     1801 tcagcatcgc atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg ccttatccgg
     1861 cctacgccag acttacttaa ccatctcttt ttcaattaac tggatcagga tatggatcac
     1921 tttaatgtga atctcctgaa tgcggtcggc ataaccaaag tgcggtacgc gaatttcgat
     1981 atccgccgtg ccagccattt tgccgccgtc tttaccggtc agggtgatca ctttcatccc
     2041 cttctcacgc gccgctgcga tcgctttgat cacgtttgca gagttaccgg aggtggagat
     2101 ccccagcagt acatcgcctt cgcgacctac cgcttcaacg tagcgggaga aaatatcatt
     2161 gaaaccgaaa tcattaccga cgcaggaaat atgactaacg tcagaaatag caatcgccgg
     2221 gtagcccgga cggttttcac ggtagcgacc ggtcaactct tcggcaaagt gcatagcgtc
     2281 gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca ccggctttaa agctgtctgc
     2341 taacaggacc gccgcgcgct gaatggcgtg aatattggcg tcatctttta aaaagttagc
     2401 cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga tcttggtaca tgaggatatc
     2461 cttcagcata aatgtaatag acaaaatgca gtgtaccgga taccgccaaa agcgagaagt
     2521 acgggcaggt gctatgacca ggactttttg acctgaagtg cggataaaaa cagcaacaat
     2581 gtgagctttg ttgtaattat attgtaaata cattgctaaa tgtatttaca tccactacaa
     2641 ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt atgatgattt
     2701 tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat
     2761 ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct ggtctgtggt
     2821 cggcgtgggt actggtgcct ctggccatta tcctcgtgcc atttaacttt gcgcctatgc
     2881 gtaagtcgat gatttccgcg ccggtatttc gaggtttccg taaggtgatg ccgccgatgt
     2941 cgcgcactga gaaagaagcg attgatgcgg gcaccacctg gtgggagggc gacttgttcc
     3001 agggcaagcc ggactggaaa aagctgcata actatccgca gccgcgcctg accgccgaag
     3061 agcaagcgtt tctcgacggc ccggtagaag aagcctgccg gatggcgaat gatttccaga
     3121 ttactcatga gctggcggat ctgccgccgg agttgtgggc gtaccttaaa gagcatcgct
     3181 tcttcgcgat
//
LOCUS       DAJTOR010000273         3184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTOR010000273 DAJTOR010000000
VERSION     DAJTOR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.278"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..865
                     /locus_tag="OO249_004065"
     CDS             <1..865
                     /locus_tag="OO249_004065"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5638152.1"
                     /translation="YADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLLSAVGSDFYGQ
                     SLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAITAEYLAQH
                     REFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCVKVRDRLNQIHTLKP
                     NRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGENSWSAPI
                     KTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNPDLSIANV
                     ISLVENAERLN"
     gene            832..1791
                     /gene="psuG"
                     /locus_tag="OO249_004066"
     CDS             832..1791
                     /gene="psuG"
                     /locus_tag="OO249_004066"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5638153.1"
                     /translation="MVSGERRTSELKISPELLQISPEVQDALKNKKPVVALESTIISH
                     GMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVS
                     RRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQEL
                     ANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDS
                     ASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKES
                     TLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            1885..3135
                     /locus_tag="OO249_004067"
     CDS             1885..3135
                     /locus_tag="OO249_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5638154.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
BASE COUNT          757 a          719 c          864 g          844 t
ORIGIN      
        1 ttatgcggat tcaaatccag gtaaaataaa atttacgcct ggtggagtcg ggcgcaatat
       61 tgcacaaaac ctggcgttgc tggggaacaa agcctggcta ctgagcgccg taggcagtga
      121 cttttatggt caatcgctgc taacgcaaac caatcaatct ggcgtttatg tcgataaatg
      181 cctgattgtg ccgggagaaa atacgtctag ttatttatca ttactcgata ataccggtga
      241 aatgctggtt gctataaatg acatgaatat tagcaacgct attacagctg aatatctcgc
      301 acagcaccgt gaatttattc agagggcaaa ggtcattgtc gcggactgta atatcagtga
      361 agaggcactg gcatggattc tggataatgc cgccaacgta cccgtatttg tcgatccggt
      421 ttccgcatgg aaatgtgtca aagtgcgcga ccgtctaaat cagatccaca ctctgaagcc
      481 aaaccgcctt gaagcggaaa ccctgagtgg gattgcgctg tcagggcgtg aagatgtggc
      541 aaaagttgct gcctggttcc atcaacatgg cctgaaccga ctggtattga gcatgggcgg
      601 cgacggcgtt tattacagcg atatcagcgg tgaaaatagc tggtctgcgc cgatcaaaac
      661 caatgttatt aatgttaccg gagcgggcga tgccatgatg gcgggacttg cttcgtgttg
      721 ggtagacgga atgccgtttg ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc
      781 gctctcctgt gaatacacca ataaccccga tttatcgatt gccaacgtta tatcgttagt
      841 ggagaacgca gaacgtctga attaaaaatt tcccctgaat tattacaaat ttccccggaa
      901 gtgcaggacg ctttaaaaaa caaaaaaccg gttgtggcgc tggaatcgac cattatttct
      961 cacgggatgc cgttcccaca aaatgcccag accgcaattg aagtagaaga aactattcgt
     1021 aaacagggcg ctgtacctgc cactatcgcc attattggcg gcgtgatgaa agtgggttta
     1081 agcaaagaag aaattgaatt actgggtcgt gaagggcata acgtgaccaa agttagtcgt
     1141 cgcgatttac cttttgttgt tgccgccgga aaaaatggcg caaccactgt ggcttcaacg
     1201 atgattattg cggcgcttgc cggaattaaa gtatttgcca ccgggggaat tggtggtgtg
     1261 catcgcgggg cggaacatac cttcgatatt tctgccgatt tgcaagaact ggcaaatact
     1321 aatgtcaccg ttgtttgtgc cggggcgaaa tctattctcg atttaggatt aaccactgag
     1381 tatttagaaa ccttcggtgt gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt
     1441 ttctgccgta ccagctcgtt tgacgtcagt attcgtctcg acagcgccag tgaaattgcc
     1501 cgtgcaatgg cggtgaaatg gcaaagcggg ctgaacggtg gcctcgtggt agcgaacccg
     1561 atcccggaac agtttgcgat gccggaggaa tctatcaatg cagccataga tcaagccgtc
     1621 gccgaagccg aagagcaggg cgttattggt aaagaaagta cactgttcct gctggcgcgc
     1681 gttgctgagc tgaccggcgg tgacagcctg aaatccaata tccagctggt attcaacaac
     1741 gccattctgg cgagtgaaat tgccaaagaa taccagcgtc tcgcgggtta atttccgacg
     1801 ggcagaaagc cgtcgcccgt actcatcaag cataccaaaa cctggcgctg acgccgggtt
     1861 tactggcatg aagggaaaat cattatggat ataatgagaa gtgttgtggg gatggtggtg
     1921 ttactggcaa tagcatttct gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt
     1981 ggagccgcac tgctgctgca aatcgctatt ggtggcatca tgctctactt cccaccggga
     2041 aagtgggcag tagaacaggc ggcattaggc gttcataaag tgatgtctta cagtgatgcc
     2101 ggtagcgcct tcatttttgg ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt
     2161 gcgggtttta tcttcgcctt tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc
     2221 agtctgctgt actacattgg cgtgatgggg ctgctgattc gcatccttgg cagcattttc
     2281 cagaaagctc ttaacatcag caaaatcgaa tcttttgtcg cagtcacaac tattttcctc
     2341 gggcaaaatg agatcccggc gatcgttaaa ccgtttatcg atcgcatgaa tcgcaacgag
     2401 ttgtttaccg caatttgtag cgggatggcg tccattgctg gttcgatgat gattggttat
     2461 gccggaatgg gcgtaccaat tgactacctg ttagcggcat cgctgatggc gatcccaggt
     2521 ggtattttgt ttgcacgtat tcttagcccg gccaccgagc cttcgcaggt cacatttgaa
     2581 aatctgtcgt tcagcgaaac gccgccaaaa agctttatcg aagcggcggc gagcggtgcg
     2641 atgaccgggc tgaaaatcgc cgctggtgta gcgacggtgg taatggcatt tgtcgcaatt
     2701 attgcgctga tcaacggcat tatcggcgga attggtggct ggtttggttt cgccaatgcc
     2761 tctctggaaa gtatttttgg ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac
     2821 tggagtgatg ccaatcttgc gggtagcctg attgggcaga aactggcgat taacgaattc
     2881 gtcgcttacc tgagtttctc cccatacctg caaacgggcg gcacgctgga agtgaaaacc
     2941 attgcgatta tctcctttgc gctttgtggt tttgctaact ttggttctat cggtgttgtc
     3001 gttggcgcat tttcggctat ttcgccaaaa cgcgcgccgg aaatcgccca gcttggttta
     3061 cgggcgctgg cagcagcaac gctttccaac ctgatgagtg cgactattgc cgggttcttt
     3121 attggtttag cttgagtttc attgccggat gtacgcgctt attaagcgtt gcatccggca
     3181 atcg
//
LOCUS       DAJTOR010000274         3177 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTOR010000274 DAJTOR010000000
VERSION     DAJTOR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.279"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..929)
                     /gene="virK"
                     /locus_tag="OO249_004068"
     CDS             complement(<1..929)
                     /gene="virK"
                     /locus_tag="OO249_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5638155.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSSSGFDREGELMLSLFYNKTVITRLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHS"
     gene            complement(934..2022)
                     /gene="capU"
                     /locus_tag="OO249_004069"
     CDS             complement(934..2022)
                     /gene="capU"
                     /locus_tag="OO249_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5638156.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTQKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            complement(2025..2867)
                     /locus_tag="OO249_004070"
     CDS             complement(2025..2867)
                     /locus_tag="OO249_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5638157.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
BASE COUNT          970 a          713 c          594 g          900 t
ORIGIN      
        1 gagtgtacag gatatgcatc caaagagcgg atagtagttt catggatata atccagcaaa
       61 gcgtatcgtt ttcggtactc agagcgtttt ttactggcta tatcgcttag cggctttcga
      121 atcacctggg ttggtaatct gtaccagtca ccacaggttt ttcctgctac agactcccag
      181 aaatcactat agaccgcaac aaaggttttg cgtttctgat accagtatct caattgccgg
      241 aaaacatgac tgtgctcact gactgccaga cactcagaga tattgcaagc tttcatcaac
      301 gcacaaaatg cttcaaaaat aatccgtttt gggaaaatcc cataacaggc ccgggttgcg
      361 caccgaataa catcaggtcc ggtattttta ggggctccct gtaatccgcc aataaaagca
      421 atgtgtccat tttgagtcag aatgactgaa aatgttaacc gggtaataac tgttttattg
      481 taaaacaatg acaacatcaa ctctccttcc cggtcaaaac cactggaaga acactggata
      541 tcaaaattct ctccatcttt cccgatgaat tggactaatg ataccgattt ttgtggaaac
      601 aaaaatttag aatatttttc tggcaaattt tgcacaaagc gataatgctc aagaatatat
      661 tgtccacggg tccaggcgcg ccccccttta tgcaaataag ggcgatgaat tcttgctggc
      721 aacgtggggt gtattgccag aatatcctca atgcacggca aggaacgcag ttcgtaataa
      781 tatcgaatac tggcaaccgg atttatagca catcgcaaaa tgtacatgaa ccggaatttt
      841 ctgtgttccc acttccccgg gagtgaatct gaggaaaaaa caatcctttt aaggaaaccg
      901 ataaatgata agttacttac agaaaacata ttgttacttg tgcttcgcta atgtgagtat
      961 ttttaacgca gttttattaa tatcaaaccg ctcttctata tcctgttttg cctggcgagc
     1021 catctgataa aaacgcccag ggtcattaaa aaaatcattc agggcgcaca tccatgcgtg
     1081 cttattacct gctggtaaca atgtcccggt ctggttgttc tgaataacat cagggattcc
     1141 accaatctga ctggccagta caggcacaga aaatgccgat gcttctgcca gtaccatacc
     1201 aaacgattcg ttttctgaag gcagaaccac cagactggca acccgatata cgggggcggc
     1261 aggaaaaaca ttgtcagcaa taaaaacatc atcatgcatg cccatactgt caatctgata
     1321 ctgtaaatgc tcccgcagtt caggcgaacc agaccccaca atcaaccaac agaactgtcg
     1381 tccattcatt tttaaatgga acagtaaatt caacataaat tcgtgccctt tttccgggcg
     1441 caacattcct acctgagcaa taacggggca tccccttcgg gacgccagcc aagaaagaac
     1501 atttggaggc aaactgtttc gggaatcaac gtataatttc tggaaatcaa agcctggagg
     1561 cacaacagta acccgggttc gacatccttc ctgctccaga tgtgtcttca tacttgttcc
     1621 gggaacaatc acctcatcgc aaaaatgatt tattgaaaaa acttttgttt tttgtgtcaa
     1681 atatgtcttt tgcctgataa ttctgaatgg atgtttccaa gtgaataacc gtactaaacc
     1741 aacaatattg ctatcatgcc cactgtgaca aacgattgca ttgggctgaa aaccatgaac
     1801 tattccgagt aatctccatg cagtagggat gtgaagactg tttcgaaata acgcgaatgt
     1861 gatatcaatt cccaatttac tggcttcaaa agcaattttg ctgttttccc tgcagacaag
     1921 cagaactgaa tgccccattt tcttcagggc cttcatttga gcaacagcct gtaattcctg
     1981 accacctata ttcggtgatg attccgtaaa tagtatattc attattaatc atccggtaat
     2041 cttggcccct tatgaaaggc cagcaacgat gaaaaaaacg gtgttgtata ataaaaaagt
     2101 cgtcttttca gccatgcgca actttcccgt tctttggtgc ttattcttcc tatccgtgta
     2161 gttcctttag caggagcatt cattcttctt tctgttgtat ataaataata gaatccaagt
     2221 tcttcagcga cctgaatata atccttgtta taataacctt ccggccaaca caaatgttta
     2281 ctgcattttc ctgtcatttc tttcaaatat tctctcccac tcaacaagtc ctgccgaaga
     2341 tgtttacact gttcttctcg tgaactgaac tttttatccc agcgtgtatg agtatgcgta
     2401 tgaacatgaa attcgaccag accagattgc aacatttcgt ttacttcaga ccagcgaagc
     2461 ataacattat cagcattccc tgtcgctatt tgatgttcgc aatcgcgatg agaatattcc
     2521 tttccaggag agtgacgaac cggaccattc ccaataaaac cggtaattaa aaaaatatgt
     2581 gctttgagat taaactcttt taataaagga tatacctgaa accagttgtc aaggtatcca
     2641 tcatcaaatg tcaacataac gcttttccgg gggagttttc cgccccgata aaaaaactcc
     2701 agttcatctg atgacagtgt tttccagtta ttttcagcca accatttcat ttgcttccgg
     2761 aatgtcacgg gagaaagtgt aaccagtccg gggcaatgac tgacatgatg atacattaat
     2821 accggaacat gattggcttt gaacaaaatg cccccttcgt ttaacattta aagtttctgg
     2881 cacctagtaa gcagagacaa aaggaaaacc caataaagct ggtgcatttt atttccagat
     2941 actggatgaa taacatagca aacccatggc atacttgata tattttacta aattaaacat
     3001 caacaattca taaacaatga tatattatca tatcttttaa aaacgactca ctaagaacct
     3061 tgaggtggca cactgaattt ggctacctga acagaggtga tatgttcacc tcagaacaac
     3121 acaggtgctc caatgaaaaa aaggaatttc agcgcagagt ttatttacgg ggtgacg
//
LOCUS       DAJTOR010000275         3156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTOR010000275 DAJTOR010000000
VERSION     DAJTOR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.280"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..156
                     /locus_tag="OO249_004071"
     CDS             <1..156
                     /locus_tag="OO249_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="catalyzes the formation of a
                     meso-diaminopimelyl-3-a meso-diaminopimelyl-3 cross-link
                     in peptidoglycan synthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5638158.1"
                     /translation="DQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQG
                     NMLRARQ"
     gene            complement(220..456)
                     /gene="lpp"
                     /locus_tag="OO249_004072"
     CDS             complement(220..456)
                     /gene="lpp"
                     /locus_tag="OO249_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5638159.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(767..2179)
                     /gene="pykF"
                     /locus_tag="OO249_004073"
     CDS             complement(767..2179)
                     /gene="pykF"
                     /locus_tag="OO249_004073"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5638160.1"
                     /translation="MKKTKIVCTIGPKTESEEILAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2736..2945
                     /gene="fumD"
                     /locus_tag="OO249_004074"
     CDS             2736..2945
                     /gene="fumD"
                     /locus_tag="OO249_004074"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5638161.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELDKQAMETVINALVK"
BASE COUNT          852 a          660 c          754 g          890 t
ORIGIN      
        1 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
       61 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
      121 ccagagcaag ggaatatgtt acgcgcgagg cagtagtggt aaatagcaga caaaaaaaat
      181 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
      241 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
      301 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
      361 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
      421 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
      481 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
      541 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
      601 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttagata aagtgagatt
      661 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc
      721 gcccatcagg gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa
      781 cagatgcggt gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacaacgt
      841 cacctttgtg tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat
      901 cagtagaagt gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat
      961 gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta
     1021 ctgcgcgagc agatttaccg ccctgagtag caaccacgat cagcggagca tccagttttt
     1081 cagcagtttc aacggcacca cggcataccg cttcggtaat gcgcagttta cggttgtcat
     1141 tgttgaactc gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga
     1201 tagaaaccgc ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcat
     1261 cagtaccgtc gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt
     1321 ttttgatcat ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac
     1381 atttttcgat catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca
     1441 ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga
     1501 ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca
     1561 ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt
     1621 ctacgccttg ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca
     1681 gagcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca
     1741 gcactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat
     1801 cgtcaaccag tacggtgttg ccaactgaca ggtcagtagt gaaaccttca tacgttaccg
     1861 caaccatttc gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag
     1921 ctttcagaga aacgtcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg
     1981 tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa
     2041 tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc
     2101 cagcgtccag cattttagct aatatctctt cagattcggt tttcggtccg atggtgcaaa
     2161 caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt
     2221 gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct
     2281 ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa
     2341 cgtgagtgcc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag
     2401 agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt
     2461 caaaaaatat acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa
     2521 aaggttacgc aaaaacttcg ctgtttgcgc aacgtctggt gccaaagttg tcagtgtgag
     2581 gataagtgaa agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat
     2641 aatttatcgc gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattgatat
     2701 atgctgatag ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg
     2761 aactgtaccg agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg
     2821 ggcaggaacc aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac
     2881 gcattcagcg cagtgagctg gataagcagg cgatggaaac cgtcattaac gcactggtaa
     2941 aatgaccatt caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc
     3001 cagttaatca ttctaaataa caactgctga aattatacga attgcggtat gtattttaat
     3061 ttataacgat aatattattt ttattttctg caatgaacgt cacaaataat tgtcgcttaa
     3121 gttaaattta tttcaaatga taataaatat cagttc
//
LOCUS       DAJTOR010000276         3114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTOR010000276 DAJTOR010000000
VERSION     DAJTOR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.281"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(9..776)
                     /locus_tag="OO249_004075"
     CDS             complement(9..776)
                     /locus_tag="OO249_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638162.1"
                     /translation="MQPCPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESLGK"
     gene            complement(779..1489)
                     /locus_tag="OO249_004076"
     CDS             complement(779..1489)
                     /locus_tag="OO249_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638163.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1505..3010)
                     /locus_tag="OO249_004077"
                     /pseudo
     CDS             complement(1505..3010)
                     /locus_tag="OO249_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT          849 a          760 c          687 g          818 t
ORIGIN      
        1 aatcattttt attttcctag tgattcggat gatgcgtcat acatcgcgca taaacactca
       61 tcaattatcc agtcatcttc ccagacctga tattctgtca ttggttcggg ctgttttagc
      121 gggttctcaa tcgcttgccg cgctctttct ttcagtaaag gatgattatt tctagcccag
      181 gcattgattt gggtggtaat gacgttatcg atatcgtcct ggttatctct gatataatcc
      241 tcacaatatc tgttcaataa acaggcaaga gaaacggtcg accaaaagcc gggaactgaa
      301 ttaatggcat ccagcaatgg cgcaagagcg atatcggcac ggaaaaacat ttccagcata
      361 tactgtaatt cgacagcttc atcgccggat acccaatcag tcatatactg gcaggcaaat
      421 cgctgtaaaa ttgcccgttc atccgcacgc cagttttctt tgttggcaag gttgatacgg
      481 cgcagagaaa aaactacgtg aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa
      541 cgcggcagaa aatagcggac ctcatcgcta ttttgcggat caggccctgg gctactattc
      601 caggcattga ttaaggaata cgggatcgct cgtaacgatg tactccgtaa tgccttttgt
      661 tcctgctgac tcaggcaata ttcacaacaa accacaaatt tctgcggcac ccgataacga
      721 cgaaaggtgt gataaatctg ctcaattaat gcctgcatgg cggggcaggg ttgcatagtt
      781 accccctcgc ccgctgatcg cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt
      841 tcaggcgaat aagggcgcta cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt
      901 ctcgacagct ttcctctgcg caattttcca ctgagtaatg acgcgtgtag agtttcagtt
      961 cggcctgctt atcgcgaaat ccgcgtaaac tgttattgtt atgaaatttc caggcatctt
     1021 taaagacctg aaagatatag acatcctcaa tccaggagtc acatttttta agcgggcgct
     1081 ttgctattat cacccgctca tccggattta gcatcgccgt aaattgtacg gtgacgtcat
     1141 ttgtcgtgtt caggtcacat tgaacatctg gtacatcact gatatagggg tcaaatccaa
     1201 aatcagcacc acaggcacaa agacggctaa tggtggcggt atcgagggaa aatcccggcc
     1261 atactttgct ggaggttaag ccaatattta atgtgacatc atatcgaagc aatccctcaa
     1321 gaccgtcagc accgacgaac ttttcccctt catccagggc ctggttaata agttgctgaa
     1381 caatgccttc atcccagtat gccgatttgc agttggcaat cgtaatatcc acgcctgtga
     1441 agggatcagt accggcaagg cttgcaaata atgtattaac taattccatt tttattatcc
     1501 agctttaagg tagcgtctta tacatcggga cataacctac atcttccacc aaactctgtc
     1561 cctgcggcgt taaaaaccac tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg
     1621 ttaccataaa ggcatcgatg atatagggat atttgccgtt gcgaatgttt tcggcagtcg
     1681 gtgcaatacc gttaatcgct agcagtttta tatttttatc ggcgttcatt tgtgttgcgt
     1741 aataacggaa ggtgtaacct attgcgttgt ttgtattacg gtattcggca acgactttaa
     1801 tcattccctc catcatactt gccacttctg tttcctgcgg cgagatcatg cggacatttt
     1861 tcatgacctg ggattgcatc accgtctggc taccagagtc ttccgggcgt tgctaggtct
     1921 ggatctcctg atcgttaccg ccaacagtac gccagttggt aattgcacca ctgaagatgt
     1981 cacgcacctg ttgttcagtc agggaattaa ccggattatc cgcattgacg atgaaaacaa
     2041 acgcttcacg ggcaaaaggc gtgtacagca aagtaatgcc cgattcctca gcgcgttttt
     2101 tctgcccacc ggaaggttgc gccacaaaaa taatatcagc atcaccttta acaatcctgt
     2161 tatacgcatc gggggtacga gagctctcca gatattccct ggtgtgaaaa tcttccggta
     2221 taacgctcaa agcataaaat gcagaggcat aaattgggta cgccgccgta gcaccatcga
     2281 gtcgcggcca gttttgcgta aactgaattt gcggtttacc acgcagtggt gtcagttgat
     2341 tattgagttt atcgggtcgc caggcccagg tatcaatatt ttcgcgcaca ccgacgccgg
     2401 gattgagctg tttatataaa taaccctgat aaccagcgac aaaaccaagc atgagaagga
     2461 taaacaggag ggaattacgg caacgtagcc cggatgtacc ggttaaagga tgtctggttg
     2521 gccggccgta ccccagcgaa aaactgatat gcgcgccaac gggtataatg actatccacc
     2581 agtactcacc tccgactact gagactaaca aaacgcccag ccaggggagc ataagatcag
     2641 ccaaccaacc cgatcccatc tggtgaaatt gataatctgc cagatgcagc gcgattatcc
     2701 agccaaccag gattaataaa ccgggtacta aaaaaagaca ttgtcgccag acggctattt
     2761 ctcttttttc tggtgcacta tcgcctgccc atactcgccc cgagaaaaat agcattaatg
     2821 cgcccagcgc cagcataaaa atgttgagca gaaggtaact tgttggcgtg ataaggatat
     2881 tacaaacttc aataataccg gcaagtccga cacccagcat agcaacaacc gccgccaaaa
     2941 attgcgtcag tatggggatg ccgaaaaaag tcattaccag cgaggacaaa atccattttc
     3001 tgttttgcat tattctttcc attctttttg aatggtgaaa ttatactccc cgagtcccct
     3061 tgccccttct ggacactttt ccgaaatgat ggcggaaaaa aacgggaccc tttg
//
LOCUS       DAJTOR010000277         3076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTOR010000277 DAJTOR010000000
VERSION     DAJTOR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.282"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..578)
                     /locus_tag="OO249_004078"
     CDS             complement(<1..578)
                     /locus_tag="OO249_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5638164.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEA"
     gene            complement(603..1481)
                     /gene="yihU"
                     /locus_tag="OO249_004079"
     CDS             complement(603..1481)
                     /gene="yihU"
                     /locus_tag="OO249_004079"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5638165.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTQAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGTPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1654..2550
                     /locus_tag="OO249_004080"
     CDS             1654..2550
                     /locus_tag="OO249_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5638166.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYNLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            2590..>3076
                     /locus_tag="OO249_004081"
     CDS             2590..>3076
                     /locus_tag="OO249_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5638167.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AV"
BASE COUNT          781 a          797 c          752 g          746 t
ORIGIN      
        1 gcctctgttc caccaagctc tgcggcggcg gcaacaatgg cgctttcacg atcaaagtcc
       61 caaccgcgac gaggtggacg caccacaggt tcaataatgc tcaccagccc ggcactacgg
      121 cagcgacgaa caaatttatc aaccattgcc agacgttcct ctgcgggttc atcttcacgc
      181 cagagtacaa gtaatttcat tgccttagcc cccatttcac gggctttatg cgggtcgacg
      241 gacatatcta tttcaacgct atcaaccggg atgccattac ctgggataaa aagatcggca
      301 gcgacaatca ggccgcaaga attagcaaca gcgccctgct cgactatttg ctcaagacaa
      361 aattgtttat ccgccagaac ggcagaagca taaggagaaa ggattcgggt cgcggcaact
      421 ttaaaatcgg tgagtacact gtcggcgatg ggttttggct gccctgctgc cgcaaacatc
      481 aagcgcatgg cctcacgctg atcgacagct aacatggcaa atccaccacc tgggcgggag
      541 atatctttca gcgtaaatgg ggtgaagtgg gatgacatag aaactcctgt taatagataa
      601 aattaaagac cagcctgctg gcgaacctga tttagcaggg cactccagtc ctgccgccct
      661 ttgccgtctt gacttgctgc agcgtaataa ctttctgctg caatccccat attgagcggt
      721 gtccccgtct cccgagcgac gtcaactgca atacgcagat ctttcaacgc cagatccacc
      781 atgaaggcgg gtgaaagatc gcctttcagc actttgccag gccaggtggt cgtaaaatgt
      841 cctttccctg ctgccgtacc actcatcacc ttaattgcga catcaagatt taatcccagt
      901 gactcacata acacagcggc ctcggaggac agcgcattaa gcgcaatgct catatagttg
      961 ttaattagct tcacgcggat ccccattcct gggccgccag cctcgaccag ctcattaccc
     1021 atgcacataa gaatcgggcg tgcacgtgca atctgctcat cagtgccacc cgctaaaatc
     1081 agcaatgtgc catcaatcgc attcacggac gttctgccca cgggagcgtc catcatactg
     1141 atgccttttt cttgtaactc ccgtattaat gcatcggtct gtaagggatg aatagtcgac
     1201 atatcaatca ataacgcgtc agatgaaaca gcctcacaga cgcctttttc ccccaacaga
     1261 acctgtcgaa caatgtcacc attgggaagc atagtgatga taaattcagc gcctgctgct
     1321 gcttccgctt gggtttgtgc cgcagtggcc ccctgcgtaa ctagcgcatc gacggcctgt
     1381 gcattgacat caaatacctg caatgaatgc cctttttgca atagattgtt agccattggg
     1441 ctacccatct gccccagccc gagaaatgca atggttgcca tgaatactct cctgtctgac
     1501 attgacaaaa taatgacaat tgaaaattcg tttttgtctg tttttgagca tatttgtgat
     1561 ttatgttcaa aaacacgaca cgcatcacta atttgatcat ttgcgtcatt taaattgacc
     1621 aaagacgaca aaattaaaca ggacaagaca aatatgacca gaattgcatg cgtgggtatc
     1681 acagttcagg acagaattta ttctttgccc actctgccgg agggcggcgg gaaatatcag
     1741 gcaaatcatt atcttgagat tggtggcggc ccggcagcaa cagccgctgt tgcaatcgca
     1801 aagcttggcg tggaggttga tttcatcggt cgcgtcggcg atgacagttg tggaaatacc
     1861 ttacttgcag aacttgaggg ttggggcgtc aataccgcat tttgccgccg atatcctaac
     1921 gcccgttcgt cgcaatcagc gatcctcgtt gatcagcatg gcgaacggat tatcgttaat
     1981 tatccaagtc ctgatctcgg tactgatgcc gaatggttag aagcgatcga tttctctcgt
     2041 tataatctga ttctggccga tgttcgctgg catagcggta ctgaaaaagc attctcgcta
     2101 gcgcgccttg cgggtgtgac aacgctttta gacgccgata tgacaccgca ggatatttct
     2161 ccacttgtcg cgcttgccga tcacgccgtg ttctcaacac cagggctgaa acgcatgact
     2221 ggcctgcagt ctcctgaaga aggtttgttc caggcaacga cacaaacagc aggtaaagtg
     2281 tatgttacgc tgggttctga aggatcgttg tggattgaag atggtcattt gtgtcaacag
     2341 gaggcatttt ctgtcaatgt ggttgatacc actggagcag gcgatgtttt tcacggcgca
     2401 ctcgcggtag cgttggcaga aaaaatgcca acaaaagagg ctattcgttt tgccagtgcg
     2461 gttgccgcga tgaaatgtac ccagcctggc ggtcgggccg gcattcctaa tcgtgagcaa
     2521 accgaatcat ttttgtcact ttatgcgtaa aatggtaaac atctaccgag gaggggcaag
     2581 ggagtattca tgagtatcat cgaagtgaca ggtaatccac gccatgacca actcgttcat
     2641 ctaatcgcag aacgcggtta tatgaatatt gaagaactgg cacaactgct ggatgtttca
     2701 acgcaaaccg tgcgccggga tatccgaaaa ctcagtgagc agggcttgat cacccgtcat
     2761 catggagggg ccgggcgcgt ctccagcgtc atgaataccg cttttgagca acgggaactt
     2821 tcgctcaccg ccgagaaacg ggcgatcgct gaggcagtcg ccgattacct tcccgaacgc
     2881 tgtaccgtct ttatcaccat cggtacaacc gtagaagccg ttgccagggc attactcaac
     2941 cggcgtgatt tacgcattat cactaacagc ctacgtgtgg cacagattct ttataagaat
     3001 caggatattg aagtgatggt gccgggaggc actttacgcg ctcataacgg cgggattatc
     3061 ggcccaggag ccgtgg
//
LOCUS       DAJTOR010000278         3057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTOR010000278 DAJTOR010000000
VERSION     DAJTOR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.283"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(51..235)
                     /locus_tag="OO249_004082"
                     /pseudo
     CDS             complement(51..235)
                     /locus_tag="OO249_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(397..471)
                     /locus_tag="OO249_004083"
     tRNA            complement(397..471)
                     /locus_tag="OO249_004083"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(436..438),aa:Arg,seq:cct)
     gene            complement(547..1479)
                     /locus_tag="OO249_004084"
     CDS             complement(547..1479)
                     /locus_tag="OO249_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5638168.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETHDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1773..2528
                     /gene="mlaA"
                     /locus_tag="OO249_004085"
     CDS             1773..2528
                     /gene="mlaA"
                     /locus_tag="OO249_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5638169.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2710..3045)
                     /locus_tag="OO249_004086"
     CDS             complement(2710..3045)
                     /locus_tag="OO249_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638170.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTEYQNEINQLS
                     APGVNFDDI"
BASE COUNT          764 a          706 c          710 g          877 t
ORIGIN      
        1 cccatctttt acggggtgaa ggtagatttc gtatgaaggg tatctgttgt ctcctgcaaa
       61 gtcataagcg gatattttcc tacggtcagt attttttcct taccgtcaat cttgtagcga
      121 agttgccata ccttttcccg gatacaggaa cataaaggta caggccatta ccatcgagaa
      181 ggcggtatgg tttttctttc ggctttgctg cttcaatctg cttaacggtg agcatgggta
      241 aaaatccggt gggtaaaatt attttatcca ctttttaccc gtcatggagt gcggctgtca
      301 acgatctgac gcgaaccatg acgaaccgtg aatatacgga aggcttgata ttcaggggat
      361 tttgcggact ggtacggatg ggagcgaact gataaatggt gtcccctgca ggaatcgaac
      421 ctgcaattag cccttaggag gggctcgtta tatccattta actaagagga caatgcggca
      481 tgagtatacc cgctaatgga ctgcggagta agtacactgc cgctcgattg cttaaaccct
      541 cgccatttat gctgggtttt tatcattttt cttaatgttt tccgcacgtt ctgctttttg
      601 gcgtgcttct gctttgcgct tgttgctcat gtcgttacgg atctgtgcat gactcattaa
      661 cgcgaagata aaggtgccgc cgcagatatt ccccgctaaa gtaggtagtg cgaagggcca
      721 aatgaaatcg ctccagtgca gcgtgccgtt aaacaccaga tagaggattt ctacagaacc
      781 gacaacgatg tgggtggtgt cacccagtgc aataagccag gtcatcaata taatcaccac
      841 aatctttgcc gcacccgctg caggaaacat ccaaaccata gtggcgatca gccagccgga
      901 aatgatcgcg ttggcaaaca tctcgctggg ggtgttcttc atcacatcca tgccgatttt
      961 gacaaatgca tcgtgagttt cttcattgaa gataggcata tattcaaatg cccatgcagc
     1021 aatacctgtc ccgagaatat tacccagcag cacgacgccc cataatcgca taagtaagcc
     1081 gacgttgctc attgtcggtt tttgcatgac gggtagtacc gcagtcacgg tgttttcggt
     1141 aaataattgc tggcgggcca taatgacgat aataaaacca aaggtataac cgagattctc
     1201 caacaagaag ctgcccggca cgccttccag ttcgacatga aatatccctt ttgccagtaa
     1261 cgaagcgccc atcgacagac ccgccgcaat ggctgaccac agtagtgcca ttgcgtcgcg
     1321 ttccagctct ttttcaccat cctggcggat atgttcatga attgccatcg cccgggaggg
     1381 gagtcgatct tcatctattt ctattttttt gccgcgctct ttttcttcgc tctcaacttc
     1441 aatttcgtcg ctgtgttgat caattttgtc gttgtccatg gtctcttctc ttgaattagc
     1501 acgtatagct aaagcgtagc ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat
     1561 gtagaaaagg ccgcgtttgc ctttttctgt ttctatagaa tcaagtagcc tacagggcgg
     1621 cgattaccag gctatgatca aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct
     1681 gttacaatcg cccacaccta aacaggcgga tacggtatcg ttccgtcatg gatggcaaac
     1741 tgcataagcc ataaaaaaac agggagacat ttatgaagct tcgcctgtcg gcgcttgctc
     1801 tgggaactac gcttctggtg gggtgtgcga gttccggtac agatcagcaa gggcgttctg
     1861 acccgttaga agggttcaac cgcaccatgt acaacttcaa cttcaatgta ttagacccgt
     1921 atattgttcg accggtcgct gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaacg
     1981 gtttgagcaa ctttaccggc aaccttgaag aacctgcggt gatggttaac tacttcttgc
     2041 agggcgaccc ttatcagggg atggtccact ttacccgctt tttcctgaac accattttgg
     2101 ggatgggcgg ctttattgat gttgcaggga tggcgaaccc gaaactgcaa cggactgaac
     2161 ctcaccgctt cggtagtacg cttggtcatt atggcgtggg ttatgggcct tacgttcagt
     2221 taccgttcta cggtagcttc acgctgcgtg atgacggtgg tgatatggca gatggtcttt
     2281 acccggttct ttcctggctg acctggccga tgtctgtggg taaatggacg cttgaaggga
     2341 tcgaaacccg cgctcagctg ctggattccg atggtctgct gcgtcagtcg tccgatcctt
     2401 atattatggt gcgcgaagcg tacttccagc gtcatgattt catcgctaat ggcggcgaac
     2461 tcaaaccgca ggaaaatccg aacgcacaag cgattcagga tgatttaaaa gatattgatt
     2521 ctgaataaga aacaaataaa aaaggtgagt cgcaaaactc accttttttg ttgttatccc
     2581 tcaattacca caagtaaaag gagataaacc gacaatgacc cggacgtaat gatgtaaaaa
     2641 tactgatgtt cgtcactgac tatttccgat attcaggcgt tgaaaaatca aaaaactgga
     2701 aaaataattt taaatatcat caaaattaac acctggcgca gatagttgat taatttcatt
     2761 ttgatattcc gtctggcgat tttgaagcct tttttcaact ttatcttcga gatccttaaa
     2821 gtctttgtat ttattttcag gatggtcaac gtcaactttg ttagcgaata aagtagaaat
     2881 tatttctttt acattatcgc gtctaaattt tgcatcgtcc acttgaaaat tatatcttat
     2941 tgctcgacct aaacgtttaa aaacttcgtt aggttctctc agtctttccc cgttgagctc
     3001 gtttacaacc tgatgatcgg aacctgttgc aaatgattct gccatgatag ctttact
//
LOCUS       DAJTOR010000279         3055 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTOR010000279 DAJTOR010000000
VERSION     DAJTOR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.284"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..891)
                     /gene="curA"
                     /locus_tag="OO249_004087"
     CDS             complement(<1..891)
                     /gene="curA"
                     /locus_tag="OO249_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5638171.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGGAGGAEKCRHAIEVLGFDVCLDHHAGDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQK"
     gene            1072..1590
                     /gene="mddA"
                     /locus_tag="OO249_004088"
     CDS             1072..1590
                     /gene="mddA"
                     /locus_tag="OO249_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5638172.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1587..2036
                     /locus_tag="OO249_004089"
     CDS             1587..2036
                     /locus_tag="OO249_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5638173.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2037..2270)
                     /locus_tag="OO249_004090"
     CDS             complement(2037..2270)
                     /locus_tag="OO249_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5638174.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2356..2529)
                     /gene="ortT"
                     /locus_tag="OO249_004091"
     CDS             complement(2356..2529)
                     /gene="ortT"
                     /locus_tag="OO249_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5638175.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2724..2819
                     /gene="yncL"
                     /locus_tag="OO249_004092"
     CDS             2724..2819
                     /gene="yncL"
                     /locus_tag="OO249_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5638176.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          744 a          777 c          719 g          815 t
ORIGIN      
        1 cttctgaaac tcatggatgc ggtgaccata atcctgagcg ataataaacc cttgcaagcg
       61 aatacgtttt ttcagcaccg ttgccatcaa caggggtaaa cgatccggac cgggtggtag
      121 ctctgtagcg ttatagctgc tcactaagcc acaaacggga atgcgcgcag atgtattaag
      181 taatggtagc actgcatcaa ataccttacc gcccacgttt tcataataga tatcaatacc
      241 ttttgggcac gctttcacca gttgttcggc aaaatcaccc gcgtggtgat caagacaaac
      301 atcgaagccc agcacctcga tagcatggcg gcatttttcc gcgccaccgg ccccccccac
      361 caccctgcaa cctttaagtt tgccgatttg ccccaccgtt gctcccacag gacctgtcgc
      421 cgcagctacc accaacgttt cgccctcttt aggctgaccg atatccagca gccccatata
      481 agcggtaaag cctggcatcc ccagcacacc cagcgaccac gatggatttt gcggatgatc
      541 gccaagtttc accagatcat caccactgga tatgtcatag tcctgccatc cactgtagcc
      601 cagcacccag tcgccaggct gataatcagg atgattcgac tccacaacat ggctcaccgt
      661 gccgccgacc atcacgccgc caatatcaac aggcggtgaa taagatggct catcgctcat
      721 acgtccacgc atatacgggt ctagggacaa ataaactgtg cgcagtaaca cctgtccttc
      781 acccggtgtg gcgacatcat cttcttcaag acggaaattc tccggaaccg gtgcgccatg
      841 tggacgcgag gccagaaccc aacgtcgatt acgctgcttt tgttgcccca taatgctctc
      901 ctgtgctttt ggcatgaatc catcaatagt agcaggctca cgggattggt tttgtttgca
      961 gccgtcacat cccctctgca acacgcaata atttcatcga ggaatccgtt gcttcgggct
     1021 gttttcgata gtattgataa catcatcatc attattttat cgaggccaga catgtccatc
     1081 cgttttgccc gcaaagccga ttgtgctgcc attgcggaaa tttataacca cgccgtgttg
     1141 tatacggcgg ctatctggaa tgaccaaacg gttgatgcag ataaccgcat tgcctggttt
     1201 gaagcgcgga ccatagcagg ttatccggtg ctggtaagtg aagaagatgg cgtagtgacg
     1261 ggatatgcct cgtttggcga ctggcgtagt ttcgatggtt ttcgccatac cgtggaacat
     1321 tcggtttatg tccatcccga tcatcagggc aaaggtctgg ggcgtaaatt gttaagccga
     1381 ttgattgatg aagcgcggga tagcgggaag catgtcatgg tcgccgggat cgaatcgcaa
     1441 aatcaggcct cgctgcatct gcaccattcg ctgggatttg tcgtcaccgc acaaatgccg
     1501 caggtgggca ctaaatttgg gcgctggctg gatctgacat ttatgcagtt gcaactcgac
     1561 gaacgcactg aaccggacgc gattggatga atcagtcgct cacccttgct tttttgattg
     1621 ccgccggaat tggtctggta gtccagaaca cgctgatggt gcgcatcacc cagacctcct
     1681 ctaccattct catcgccatg ttgctgaact cactggtggg gattgtgctc tttgtttcta
     1741 ttttatggtt taaacaaggc atggcggggt ttggcgaact ggtgtccagc gtgcgctggt
     1801 ggacacttat acccggcttg ctgggatcgt tttttgtctt cgccagtatc agcggatatc
     1861 agaatgtggg agcggcgaca actattgcag tactggtcgc cagccagcta attggcggtc
     1921 tgatgctgga tatcttccgc agtcatggcg taccactacg ggcgttgttc ggcccaattt
     1981 gtggcgcaat tttacttgtg gttggcgcct ggttggtagc cagacgctca ttttaatcag
     2041 aggatggtgc cgcctttggt taactgttcg tggcgcgctt ccatatcttc tttatgcttg
     2101 cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct gggtgtagcg atcttcccga
     2161 cttaactccg cgtcatcgct cagggcgatt aataattcgt tcatatgggc aatgacgctc
     2221 tcttcaatcg cggcatccac gcgcgcgatg acttcatcca gatgcgacat actttctcct
     2281 taaccacttg tgaaatttat cgattcatcc aaatgatata agtctgcgtg gtgccgtcaa
     2341 gccagtgagt aagatttaaa acagtgaaaa caacagcgcc accgggaaac tcatcggcca
     2401 tgttgccccc accagaaagg cgcttaagaa acggatgcgt ttcttatcgt gagaaagaaa
     2461 ccaggtgata agaaatgcga ttgctgccat aaccgcataa aaaacaagca tatgttgata
     2521 gagagacatg tacgggcgtc cagccagatg aataaaacgc gcgcaaatat gcgtcatttt
     2581 ttgacgtgca tcaagttttt tcaccagttt gagaaaaaat catcaaacag caacttgccc
     2641 ttatccatct tcagaatgag tattattgcg gattttctta acccgtacta tacacatagg
     2701 ggcaatgata aaaggtggca aaaatgaatg tttccagtag aactgtagta ctgataaatg
     2761 tctttgctgc tgttggtttg tttactctta tctctatgag atttggctgg tttatttgat
     2821 gtagtgattt tgcacgcagc acgtactgtc agagaaatca actctctgac agtacgcaag
     2881 atcttctcat actctccagg tttacaacat tatccttaat atttacttct ttttgctggc
     2941 atctacattg aaatgagtag aaaacatccc cctactgtcg tctatacaaa cagcttactg
     3001 attcaaacgg aatcttgttt tcacattttt ctatcatgaa tactttatac gcgcg
//
LOCUS       DAJTOR010000280         2978 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTOR010000280 DAJTOR010000000
VERSION     DAJTOR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.285"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..141
                     /locus_tag="OO249_004093"
     CDS             <1..141
                     /locus_tag="OO249_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="activator of the allDC-ylbA operon involved in
                     allantoin utilization; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5638177.1"
                     /translation="MRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNP
                     RS"
     gene            210..1304
                     /gene="mnmH"
                     /locus_tag="OO249_004094"
     CDS             210..1304
                     /gene="mnmH"
                     /locus_tag="OO249_004094"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5638178.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            1420..1785
                     /locus_tag="OO249_004095"
                     /pseudo
     CDS             1420..1785
                     /locus_tag="OO249_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2347..2796)
                     /locus_tag="OO249_004096"
     CDS             complement(2347..2796)
                     /locus_tag="OO249_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638179.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFSHVAVDISIYTNVEDDYFFSYFSLTNDDGGRFKKTLTVR
                     AREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFLLVKLMLRIIL"
BASE COUNT          787 a          683 c          694 g          814 t
ORIGIN      
        1 atgcgccctc cttcgccatt gagtctggca tggcgcaaat ttggcagcgg caaagcggta
       61 gaagatattg tgaccttgtt tacccagcgc aggccggaaa tcagcggatt tttagaaatt
      121 ttcggcaacc cacgcagtta aaaatcaagt ttccttgcgc gttggcgcgt aaaatagcgc
      181 gcaagctgat ttgaggttca cttttaacta tgcaagagag acacacggaa caggactatc
      241 gtgccctgct gattgctgat acgcccatta ttgatgttcg cgcccctatc gagtttgagc
      301 aaggcgcaat gcccgccgct atcaatctgc cgttaatgaa taacgatgaa cgcgccgccg
      361 ttggcacctg ctataaacag caagggtcag acgcggcgct ggcgctgggg cataaactgg
      421 tggcgggtga aattcgtcag cagcgcatag acgcctggcg ggcagcgtgc ctgcaaaatc
      481 cgcaaggtat tctctgctgt gcccgtggcg gtcaacgctc acatattgtg caaagctggt
      541 tgcatgcagc ggggattgat tatccgctgg tggaaggcgg ttataaggca ctgcgccaga
      601 ccgcgattca ggcgactatt gaactggcac aaaaaccgat agtgctgatt ggcggttgta
      661 ccggcagcgg taaaacgctg ttagtgcagc aacagccgaa cggtgtcgat ctggaagggc
      721 tggcgcgtca tcgcggttcg gcgtttggtc gcacgttaca accacaactt agccaggcga
      781 gttttgaaaa tctgctggct gccgaaatgc taaaaaccga cgcccgtcag gatttgcgcc
      841 tgtgggtgct ggaagatgaa agccggatga tcggttcgaa tcacctgccg gaatgtctgc
      901 gcgagcgaat gactcaggcg gcgattgcgg tggtagaaga tccgtttgag atccgtcttg
      961 aacgcctgaa cgaagagtat ttcttgcgta tgcatcatga ttttacccac gcgtacggcg
     1021 acgaacaggg ctggcaggaa tattgcgaat acctgcatca cggactttcg gctattaagc
     1081 gtcggctggg gttacagcgc tataacgaac tcgctgccca gctggatacg gcgctgacaa
     1141 cgcaactcac caccggcagc accgacggtc atctggcctg gctggtgccg ttacttaaag
     1201 aatattacga cccaatgtat cgctatcagc ttgagaaaaa agcggaaaaa gttgtctttc
     1261 gcggtgagtg ggcggaagtg gcggaatggg ttaaggcgcg gtaatgaggg acgtatttgt
     1321 attagtcgcg acttgatctg acacatggcc ttgaaaggtt gagagttacc ggttttgata
     1381 tgagtgtcga atccttatcc aaaacaagag gtaactctca tgcttcatac tgccaatccc
     1441 gtcatcaaac acaaagccgg tttgctcaat ctggctgaag aactcagcaa cgtatcaaaa
     1501 gcctgtaaaa tcatgggcgt ttcgcgtgat acgttttatc gttaccgcga actggtcgct
     1561 gaaggcggtg tggatgcgca gattaatcgt agtcgccgcg ctcctaacct taagaaccgt
     1621 accgatgagg caactgaaca ggctgttgtt gattacgccg tcgctttccc gacacatggt
     1681 cagcaccggg ccagcaacga gctgcgtaaa caggggggtt ttatctccga cagtggtgtc
     1741 cgttctgtct ggttactcca taaccttgag aatctcaaaa gacgatactg aacaaatttt
     1801 atcaggtgac gttccgtaaa aagttgtatg gagttctcga tgcagtcggc gcgactatca
     1861 gtacccccgc tgaaccctgg actatcgggc cgccgtactg gggcatgtca ccctggcgtg
     1921 ccgctggttt tccgctaatg gtttatttcc tctttctttc ccggcaagaa tttattttaa
     1981 tccggaagga ttatttattt tcagataggg actcacatta aacacgtaac attttaatta
     2041 atgcatggct gaatatgtat tacacaatgt cagatatcgt aaaagaccag tgcaattcta
     2101 tgtaaactct ctgactgttc attttatttg ttgtttcagg gtcggaactc accctcaata
     2161 aacaactgaa tacgagtaga tattcatcgt ctgagctata tggctttaca caatagccga
     2221 catgtctttt ctcagtcagg aattttactt ttcctcacta cgaagttgac gacttcgata
     2281 tgggatagac tcttaattca agcaattact ctgcacaagc atcaggcaat ccagctccaa
     2341 tatttgttat aaaataatgc gtaacattaa tttcaccaac aaaaaaaggt ttacaccatc
     2401 cccttgccct ggtatcttac catatttaca ccacactttt tttgtatctg gatcattctt
     2461 tgatacgatt ttcgccactt gttccctggc cctcactgtc aatgttttct taaacctacc
     2521 cccatcatca tttgttaggg aaaaataaga aaaaaaataa tcatcttcta catttgtgta
     2581 tattgatata tccaccgcta cgtgcgaaaa aaaatccggg agccatgtaa aaactccatc
     2641 attttctggt attgccatat agtttatgta atgtttactt acaagagatt catctttgta
     2701 taaataaaga taagtaatta cgcataaaac aacaatgatt ataatagcaa aaataaatat
     2761 tatcatcttt gatagattac ttgagatagc cagcatcttg taaagccttt atcgtttttt
     2821 tatgctctgg attaatataa tcactacatc tatctgagca atctgttgtt gatggacatg
     2881 tcaacccatg gtcatttaca gccaagtctt ttttattatc ttcaatcatt tcagaatggg
     2941 atagctttac ttttctgccg tacataccga acagattt
//
LOCUS       DAJTOR010000281         2811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTOR010000281 DAJTOR010000000
VERSION     DAJTOR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.287"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..319)
                     /locus_tag="OO249_004097"
     CDS             complement(<1..319)
                     /locus_tag="OO249_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638180.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSG"
     gene            complement(385..729)
                     /locus_tag="OO249_004098"
     CDS             complement(385..729)
                     /locus_tag="OO249_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCR5638181.1"
                     /translation="MTEADLYPHLAHLAGGQVSPYVAPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(726..1172)
                     /locus_tag="OO249_004099"
     CDS             complement(726..1172)
                     /locus_tag="OO249_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCR5638182.1"
                     /translation="MIEILLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRTPVRRGKLRRNVVILSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(1169..1489)
                     /locus_tag="OO249_004100"
     CDS             complement(1169..1489)
                     /locus_tag="OO249_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5638183.1"
                     /translation="MISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGRSSRERMQSGA
                     EMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDATGGRLEILCRL
                     GGEK"
     gene            complement(1529..1855)
                     /locus_tag="OO249_004101"
     CDS             complement(1529..1855)
                     /locus_tag="OO249_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5638184.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVLREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1852..2451)
                     /locus_tag="OO249_004102"
     CDS             complement(1852..2451)
                     /locus_tag="OO249_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5638185.1"
                     /translation="MPAGKSLTPDDVRPMLEQMVTEAVSHIPVPRDGRDYDPDVLQKA
                     VLDAVSALPAPQDGRDATALEILPAIDVQKSFPRGTYATHQGGLWRAYEKTHGMRGWE
                     CLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTV
                     TWGGSLWHCNSMTEDKPREAHSSAWTLAAKRGRDAGGGK"
     gene            complement(2442..2811)
                     /locus_tag="OO249_004103"
                     /pseudo
     CDS             complement(2442..2811)
                     /locus_tag="OO249_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
BASE COUNT          611 a          899 c          692 g          609 t
ORIGIN      
        1 taccggacgg aaacagcacc ttaaacgcat acaacgcgtc attgtcatag gcatcacgca
       61 gggcgttctg ggcctgattc agataaaaat tacccgacat ggaaatctca gacgacgccc
      121 ccagaccgtt gatgttctcc tgctctgtgg agcagagcgt ggtcacatca atatcctgtt
      181 tctgaccggc ggtgaactgg acttccttga tggtgcagtc caggcgcaga tattccgcct
      241 tatccatagt ttcagcagtc gccggggcag atgaaatcat cacctgcgtc agctgtgagc
      301 gttcatacaa agcagacatt ctgcctcctg ataataaaaa acccgcacgc ggcggggtat
      361 ggttttgtag aaaaaaagaa aaagtcacac cgtgacctga aactccaggg ttgcacggta
      421 acagcggttt tccggaatat agtcctgcat ttcactgacg gatcccgggg ccagcagcat
      481 tatggcttca cgggcgtcct gacgtatctg acgcgcctgc gtcacagtcc cggcataaac
      541 gtctatctgc accgacactg aggactccgc ctgcccgccc atcacgtccg ctgacaccga
      601 tgaaatcagg ctgaaaacca cccacggaag cgccaccgac ggcctgccat ccagcagggg
      661 ggccacatac ggggacacct gcccgccggc aagatgcgcc agatgaggat acaaatccgc
      721 ctccgtcatc gtctcagtac ctcatcaatg gcccggttca tccgcgcaat cgccacctga
      781 gctgcctgtt cactgcgcac atcaaacgcc gggcgcacaa acgggtgcgg tggcatattc
      841 acggtcccca tttccacaaa ccgccagtag aaagcattgc gcgggttatc cgccttcatg
      901 gtgttatcgc tgttaccggt gtccggatta acaccacgga tatggacacc ggattccatc
      961 ccgccatcgc gggagcgccg ggaaaggatc accacattgc ggcgcagttt tcccctgcgt
     1021 accggtgtcc gtgacaccac ttcttctttc agcacattcg cacccgcacg ggttgcctca
     1081 cgcagcaccc ggttattttc cgcaccactc agaagctgca aatcgcggct gatgtcctcc
     1141 agccccgaaa aatccagcag gatttcgatc atttttcccc tcccagccga cagagaattt
     1201 ccagacgccc gccggtcgca tccggcacgg gcagcccgac aacgttcagg atccggtcac
     1261 gccatggacc actcagcaca tgaagtcgtg acgctgccgt gatttcccga ccggactgac
     1321 cgcgcaccca gatgcggatt tccgcctgcg ccatttccgc accggactgc atccgctccc
     1381 ggctgctcct gcctcggata tccgcatgaa ttttcccgca tgacacccat tcttccgtca
     1441 tttctccggc agcgttacgg gttaacaccg ggttcagaac acttatcatc tatgtcagac
     1501 gacctgcaga tattgccatt ccccctcctc ataacaccgt cggacaacgc aaatcgtaaa
     1561 tcagcacgga aacagaaaac ggcagctccc cctgaagcag ttcttcccgc tccgcaagat
     1621 ccggattccg gtacagcatc ccggtcagtc gcatggcagc ccccttcatc cgggttaatg
     1681 cctcgcccgg gatcagttca ccgtcctcac ggagcacttt atcccggctg ccctgaatgt
     1741 aggccagcag cacggcggta gcctgacgaa ccttgtccat cagcatgtca tcatccgcgt
     1801 catggtcgac acgcagatgt gccttgatct cttccagtgt cagtaatgcc gtcattttcc
     1861 gcctcctgca tcccgcccac gttttgcagc cagggtccag gctgatgaat gagcttctcg
     1921 gggtttatct tcggtcatac tgttgcagtg ccacagcgag cccccccacg tcaccgtatc
     1981 gccggggtgg taggtttcac cggctctgaa cacaccgcgg tagagcatca ccggcaggga
     2041 aaatgttttt tccgtacgct ggccactgct ctgccggacc accacagaga acaaccgctc
     2101 acccgtcatg ctgacgtcaa tatccgccac cccgtcaacc aggcattccc atccccgcat
     2161 cccgtgcgtt ttttcatacg cccgccagag tccgccctgg tgtgtggcat acgtgccccg
     2221 gggaaaggat ttttgaacgt caatggcggg gagtatttcc agagccgtgg catcacgccc
     2281 gtcctgcgga gccggcaggg cactcaccgc atccagaacc gccttctgca gaacatccgg
     2341 atcgtagtca cgaccatcac gcggaacagg gatatggctt acagcctccg tcaccatctg
     2401 ttcaagcatc ggacgcacat catccggggt gagactttta ccggcaggga tgctttcacc
     2461 atatacgaac cgtttctttc gggcgtcatg gtgacctcgg ctacaccctg cgtattagta
     2521 gagaccgtat tctggctgat gatcatttgc gaggatggct ctgcactgaa tgtcacgggt
     2581 tgatgcgcaa ccgggttgcc aaactggtcg ttaacgtgag ctttaactgc tacgtcttgt
     2641 gcgaccatta cctgaccttc cggtatctcc agactggtaa tcgtcgcaga attaacatct
     2701 gcactggcat tcagtaatcg cgagatgact tcagtaggtt tatctgccag agaggctgaa
     2761 actgttttca gtccgacctc ggtgcttgtc acaagaattt cagcgaaacc c
//
LOCUS       DAJTOR010000282         2783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTOR010000282 DAJTOR010000000
VERSION     DAJTOR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.288"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..579
                     /locus_tag="OO249_004104"
     CDS             <1..579
                     /locus_tag="OO249_004104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638186.1"
                     /translation="KKNNVPMIQNNGNLQPFSMGNLPSIAKTFPMEIHTTILQQLNPL
                     EHISNAISRICYYKEQCKALELESLKIKEQASFMHLKIDTNFKKAMYELETRRKSNEL
                     KLIAAANDFKQISITKSDLIKEISKLVSSICSNDVSLESKKAMNDTLIIMKGMLSDQR
                     SESYEKFKSLLSSIDSSYNDALLIEDHQDNYV"
     gene            572..1153
                     /locus_tag="OO249_004105"
     CDS             572..1153
                     /locus_tag="OO249_004105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638187.1"
                     /translation="MFNIILGVAAVGVGLYILNELDDRAGEEKKRWENQRESVCKSIE
                     EHKRYIEQHVEYVQSMYDFHQLVDLHYSCMKVANEAYKLLNDAKKSMGAIYDAINKLK
                     AHRSKLFNDKKESDDYEKKKEIQAEINMIKEIIDGLYSELDLIKKDKEHFYQELKAFN
                     LKTSSIKYRIRDNTGYKGFDWYNRLQERTLLRR"
     gene            2133..2609
                     /locus_tag="OO249_004106"
     CDS             2133..2609
                     /locus_tag="OO249_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5638188.1"
                     /translation="MFIDSEKRLKQLSDEAKKSTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          907 a          545 c          624 g          707 t
ORIGIN      
        1 aaaaaaaata acgtgccaat gatacaaaat aatggaaatc tccaaccatt tagcatgggt
       61 aatttaccat ctattgctaa aacattccca atggaaatcc atacaactat acttcaacag
      121 cttaacccat tggagcatat atccaatgct ataagtagga tttgttacta taaagaacag
      181 tgtaaagcac ttgagcttga gtcactaaaa ataaaagaac aagccagttt tatgcacctt
      241 aaaatagata ctaattttaa aaaggcaatg tatgagctag aaacaagaag aaagagtaat
      301 gagttgaagt tgatagctgc tgcaaatgat tttaagcaaa tatcaataac aaaatcagac
      361 ttaattaaag agatatcaaa gcttgtttca agcatatgta gcaatgacgt ttcgttggaa
      421 agtaaaaagg cgatgaacga tactcttatc attatgaaag gcatgctcag tgatcaacgg
      481 tctgagtcat atgaaaaatt caaatctttg ttaagtagta ttgattctag ttacaatgat
      541 gcgttgttga tagaggatca tcaggataat tatgtttaat atcatactcg gagttgctgc
      601 tgtaggtgtt ggtttatata tcttgaacga acttgatgat cgcgccggag aagaaaaaaa
      661 gcgatgggaa aatcaacgag aatcagtatg taaaagcatt gaagaacata agagatatat
      721 agaacaacat gtcgaatatg ttcaatcaat gtatgatttt catcagttag ttgaccttca
      781 ttattcatgt atgaaagtgg caaatgaagc atataaactt cttaatgatg ctaaaaaatc
      841 aatgggcgct atttatgatg ctatcaataa actcaaagct caccgctcta aattatttaa
      901 tgataaaaaa gaaagcgatg attatgaaaa gaaaaaagaa atacaagcag aaataaacat
      961 gatcaaagaa atcatagatg gcctatactc tgaattagat ttaattaaaa aggataaaga
     1021 acatttttat caggaattaa aagcttttaa cttaaaaact agcagcatta aatacagaat
     1081 tagagacaat accggatata aaggctttga ttggtataat cggttacaag aacgaacttt
     1141 acttcgacgt tagttttgca ctgaaactat atcagataac aatcctccac ggttccggca
     1201 gcgccttgag cgcaaccgga aaccgtctgg ggagaacccc agacccccat cgctgcaaca
     1261 tcgacggtct gcaccacctg ccaaccgagt aactgcctgt tccgtgcgaa tgggctttgc
     1321 agggggctgg cgcggttcct ggcgacctga aagcccctgg ttaggggtat cggcgcgcct
     1381 gacctcctga gagcctctgt aacgcatttt cgcgcctccc atgccctggc gggcatttaa
     1441 aacccgtttc gtgcgtctga gagcgtttga agggctatct gacgactcat ccccgaaaga
     1501 acaacgacgg aatcaccgac aacatgcgag aggtggcacc gctcgccgca gccccagcga
     1561 ccgaacgtta gtgagcgagt gggcgaggaa gcggaagaga accggtagtt agattgagca
     1621 cgtacgcact gatgggtgtc ggggcgcagc cctgaccaga tggcaaacgg aacatcgtcg
     1681 tgggtgacgg tattacattt gcacatcctg tcccgatttt tgaggcattt tagccttcat
     1741 ccccctttta gtggtgaacg gacggcaaaa acatgatgga actgttgaac gtggagagcg
     1801 cgaaatcgga caaaaatcac ggttgacgag gcttgcagag gagtaagcca gtatacactc
     1861 cgctatcgct acgagactgg gtcagggctg cgccccgaca cctccaaagg gtgttgcgtt
     1921 gagcgcaaca cccttgccta gaatagatct tttacgccga tttgtaagtt gttgtttttt
     1981 tagtgtgttt tttgtttacc tgttgcatcc agggtgacac aacgttgtcg catccaggat
     2041 gcgacaacac gcaacaaggt gttgcacaga tgtatcatga tggcttagat ttgaaccaac
     2101 gttagtgcat gggattttca gagggaaaaa tcatgtttat tgattcagaa aaacggctga
     2161 aacaactttc agatgaggca aagaaaagca ccgaggatct cgaagaagca aagaagaatt
     2221 caaggtttac acaggtatcc ccaaaaggtt gggaacgcgt tcgagagctg ctgaaggata
     2281 gccaaggcat atcagcactg aagctgtact catttttagc ggagcatatc gatccaacgt
     2341 gtggcgctgt cgttgcggat cagcaattcc tagctgaaaa acttggagtt agcagaagca
     2401 caattattcg gtggctcaat tacttagaat caaaaaatgc attagttaga atccccgttg
     2461 ctggtaaggt ttgtgcgtat gccctcgatc cacatgaagt ctggaaggga tacaacacta
     2521 cgaaaaacca tgcagcgttt gtcactaaaa cactggtcaa caaagacggt gatattcagc
     2581 gccgaatcat ggccatgttt tcaaattgag ccagcggcag gcggacaatc aggggctacg
     2641 tgttaacgtt ttgaccatga ttgtctatcc tgcattgctc ttttaccacc tagacagcac
     2701 catagtgata agataaacat gctcaaacgt tttatgtaac attagaaaaa ttcagttaat
     2761 taaaaaaggt tttctaaatg agc
//
LOCUS       DAJTOR010000283         2757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTOR010000283 DAJTOR010000000
VERSION     DAJTOR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.289"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1280
                     /gene="torZ"
                     /locus_tag="OO249_004107"
     CDS             <1..1280
                     /gene="torZ"
                     /locus_tag="OO249_004107"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5638189.1"
                     /translation="FGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIP
                     VARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIV
                     VSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDV
                     FADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIE
                     MRRSEKNEQYVRYADFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDE
                     WKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFGIANGD
                     LVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQL
                     ANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
     gene            complement(1445..2416)
                     /gene="cmoB"
                     /locus_tag="OO249_004108"
     CDS             complement(1445..2416)
                     /gene="cmoB"
                     /locus_tag="OO249_004108"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5638190.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2413..>2757)
                     /gene="cmoA"
                     /locus_tag="OO249_004109"
     CDS             complement(2413..>2757)
                     /gene="cmoA"
                     /locus_tag="OO249_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5638191.1"
                     /translation="TLQFLEPSERQALLDKIYQGLNPGGALVLSEKFSFEDAKVGELL
                     FNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARLHKAGFEHSELWFQCFNF
                     GSLVALKAEDAA"
BASE COUNT          705 a          684 c          722 g          646 t
ORIGIN      
        1 gttttggttt ctcttatcat tactccaatg gtggcaaccc gacacgtgtt ggcggcgtgc
       61 tgccggaaat gtctgcggcg attgccggac aggccagcga ggccgctgat gatggcggaa
      121 tgagggcgat tcctgttgcc cgtatcgtcg atgcgctgga aaatcctggc ggcaaatatc
      181 agcataacgg taaagagcaa acgtatccga atatcaaaat gatctggtgg gcgggtggcg
      241 gtaactttac ccatcaccag gatactaatc gtctgattaa agcatggcag aaaccggaga
      301 tgatcgtcgt ttctgaatgc tactggaccg cagctgctaa acatgcagat atcgtattac
      361 cgatcaccac atcgtttgag cgcaatgact tgacgatgac cggtgattac agcaaccagc
      421 atattgtgcc gatgaagcag gctgtcgctc cgcaatttga agcgcgaaac gattttgatg
      481 tgtttgccga tcttgcggaa ttactgaaac ctggcggaaa agagatctat accgaaggta
      541 aagatgaaat ggcgtggctg aaatttttct atgatgccgc tcagaaaggt gcccgtgcgc
      601 aacgcgtgac tatgccgatg tttaatgcct tctggcagca aaataaactg atcgaaatgc
      661 gccgcagcga gaagaacgaa cagtacgttc gttatgctga tttccgcgcc gatccggtga
      721 aaaatgcgct gggtacgcca agcggcaaaa ttgagattta ctccaaaacg ctggaaaaat
      781 ttggctataa ggattgcccg gcacatccaa cctggcttgc gcctgatgag tggaagggta
      841 ccgccgacga gaagcagttg cagcttctga ccgcacatcc ggcacaccgt ttacatagtc
      901 agcttaacta tgcggaactg cgtaaaaaat atgcggttgc agatcgtgaa ccaatcacta
      961 ttcataccga agatgctgct cgctttggta ttgcgaatgg tgatctggtg cgtgtgtgga
     1021 acaaacgtgg tcagattctg acaggcgcgg tggtgactga cgggatcaaa aaaggcgtgg
     1081 tatgcgtgca tgaaggtgca tggccagatc tggaaaatgg cttgtgtaaa aacggcagtg
     1141 cgaacgtgtt aacggcggat atccccagct cgcagctggc aaatgcctgt gccggtaact
     1201 ctgcgctggt gtatatcgaa aaatatacgg gtaacgcacc gaagttaacg gcatttgata
     1261 agcctgctgt tcaggcgtaa gaagtgaata gcgcccggaa acgggcactg aggttatgca
     1321 aacgtaaaac tgcctgatgc gctatgctta tcaggcctac atagtccctg caatagattg
     1381 aattagcaag ggtttgtagg ccggataagg cattcacgct gcatccggca gtaataccag
     1441 acctttacgg cttgcgcgca atcagcaccg cgcgtttagg cgcaggataa ccttcaactg
     1501 tcttactcgg atcatgtggg tcgaggaaat cggacagaga ttcggtaacc atccattcag
     1561 tgcgtcgctg ctcttcagta gtggtaacgc acacatccac aacgcggata tctacaaaac
     1621 cacacttctt cagccagttt ttcagcgcca gcgcggaagg gatgaaatag acattacgca
     1681 tttgcgcgta acgatcgcct ggtaccagca ccgtgttctc atcgccatca ataaccagcg
     1741 tttccagcac cagttcaccc tcattcacca gttgatcttt caactgccag agatgctcca
     1801 gcggcgaacg gcgatggtaa agcacgccca tcgaaaagac ggtatcaaag gctttcagtg
     1861 ccggaagctg ttcaatacct aacggtaaca aatgtgcgcg ctgatcgtta cccagtagtt
     1921 tacgcaccgc ttcaaactgg cacagaaaca gttgcgtagg gtcgatcccc accgcgaggt
     1981 gcgcgcctgc gccaatcatg cgccacatgt gataaccgct gccgcagccg acatccagaa
     2041 tggtgcgtcc ggttaaatca gaaagatggg gcagaacacg atcccatttc cagtcggaac
     2101 gccattcggt atcgatgttg acgccataca gtgagaacgg ccctttgcgc catggcatca
     2161 ggttccgcat aagcgtttca atgcgtttaa tttgcccggt gctcagtggc tcttcgcttt
     2221 cggcggttac gctatgcaat aaatccagac gatacggttt aatttcaggc agaaattcca
     2281 ccgcgttgga ccactgctta aacagcccgt gctgctgctc gcgctgccag ttagcaatct
     2341 gcgcgggcag cgtttcgagc cagtgtgaaa gatgattttt ggcaatcaga gaataaaagt
     2401 taccaaagtc gatcatgcag cgtcctctgc ttttaatgcc accagtgaac caaagttaaa
     2461 gcactggaac cacagctcgc tatgctcaaa accggcttta tgcaggcgtg ctttatgggt
     2521 ttccacggaa tcggtcagca tcacgttttc cagcatgctg cgcttctggc tgatctccag
     2581 ttcgctgtaa ccgttggcac gtttaaagtc gtggtgcatg ttgaacaaca gttcaccaac
     2641 tttggcatct tcgaaactga atttttccga aagcaccagc gcgccgcccg ggttcagtcc
     2701 ttgataaatt ttatccagta atgcctggcg ctcggaaggt tccaggaatt gcagggt
//
LOCUS       DAJTOR010000284         2750 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTOR010000284 DAJTOR010000000
VERSION     DAJTOR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.290"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..2552
                     /locus_tag="OO249_004110"
     rRNA            <1..2552
                     /locus_tag="OO249_004110"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2648..>2750
                     /gene="rrf"
                     /locus_tag="OO249_004111"
     rRNA            2648..>2750
                     /gene="rrf"
                     /locus_tag="OO249_004111"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          713 a          606 c          865 g          566 t
ORIGIN      
        1 catattgtga gctcgatgag tagggcggga cacgtggtat cctgtctgaa tatgggggga
       61 ccatcctcca aggctaaata ctcctgactg accgatagtg aaccagtacc gtgagggaaa
      121 ggcgaaaaga accccggcga ggggagtgaa aaagaacctg aaaccgtgta cgtacaagca
      181 gtgggagcac gcttaggcgt gtgactgcgt accttttgta taatgggtca gcgacttata
      241 ttctgtagca aggttaaccg aataggggag ccgaagggaa accgagtctt aactgggcgt
      301 taagttgcag ggtatagacc cgaaacccgg tgatctagcc atgggcaggt tgaaggttgg
      361 gtaacactaa ctggaggacc gaaccgacta atgttgaaaa attagcggat gacttgtggc
      421 tgggggtgaa aggccaatca aaccgggaga tagctggttc tccccgaaag ctatttaggt
      481 agcgcctcgt gaattcatct ccgggggtag agcactgttt cggcaagggg gtcatcccga
      541 cttaccaacc cgatgcaaac tgcgaatacc ggagaatgtt atcacgggag acacacggcg
      601 ggtgctaacg tccgtcgtga agagggaaac aacccagacc gccagctaag gtcccaaagt
      661 catggttaag tgggaaacga tgtgggaagg cccagacagc caggatgttg gcttagaagc
      721 agccatcatt taaagaaagc gtaatagctc actggtcgag tcggcctgcg cggaagatgt
      781 aacggggcta aaccatgcac cgaagctgcg gcagcgacgc tgatgcgttg ttgggtaggg
      841 gagcgttctg taagcctgtg aaggtgtgct gtgaggtatg ctggaggtat cagaagtgcg
      901 aatgctgaca taagtaacga taaagcgggt gaaaagcccg ctcgccggaa gaccaagggt
      961 tcctgtccaa cgttaatcgg ggcagggtga gtcgacccct aaggcgaggc cgaaaggcgt
     1021 agtcgatggg aaacaggtta atattcctgt acttggtgtt actgcgaagg ggggacggag
     1081 aaggctatgt tggccgggcg acggttgtcc cggtttaagc gtgtaggctg gttttccagg
     1141 caaatccgga aaactaaggc tgaggcgtga tgacgaggca ctacggtgct gaagcaacaa
     1201 atgccctgct tccaggaaaa gcctctaagc atcaggtaac atcaaatcgt accccaaacc
     1261 gacacaggtg gtcaggtaga gaataccaag gcgcttgaga gaactcgggt gaaggaacta
     1321 ggcaaaatgg tgccgtaact tcgggagaag gcacgctgat atgtaggtga agtccctcgc
     1381 ggatggagct gaaatcagtc gaagatacca gctggctgca actgtttatt aaaaacacag
     1441 cactgtgcaa acacgaaagt ggacgtatac ggtgtgacgc ctgcccggtg ccggaaggtt
     1501 aattgatggg gtcagcgcaa gcgaagctct tgatcgaagc cccggtaaac ggcggccgta
     1561 actataacgg tcctaaggta gcgaaattcc ttgtcgggta agttccgacc tgcacgaatg
     1621 gcgtaatgat ggccaggctg tctccacccg agactcagtg aaattgaact cgctgtgaag
     1681 atgcagtgta cccgcggcaa gacggaaaga ccccgtgaac ctttactata gcttgacact
     1741 gaacattgag ccttgatgtg taggataggt gggaggcttt gaagtgtgga cgccagtctg
     1801 catggagccg accttgaaat accacccttt aatgtttgat gttctaacgt tgacccgtaa
     1861 tccgggttgc ggacagtgtc tggtgggtag tttgactggg gcggtctcct cctaaagagt
     1921 aacggaggag cacgaaggtt ggctaatcct ggtcggacat caggaggtta gtgcaatggc
     1981 ataagccagc ttgactgcga gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga
     2041 tccggtggtt ctgaatggaa gggccatcgc tcaacggata aaaggtactc cggggataac
     2101 aggctgatac cgcccaagag ttcatatcga cggcggtgtt tggcacctcg atgtcggctc
     2161 atcacatcct ggggctgaag taggtcccaa gggtatggct gttcgccatt taaagtggta
     2221 cgcgagctgg gtttagaacg tcgtgagaca gttcggtccc tatctgccgt gggcgctgga
     2281 gaactgaggg gggctgctcc tagtacgaga ggaccggagt ggacgcatca ctggtgttcg
     2341 ggttgtcatg ccaatggcac tgcccggtag ctaaatgcgg aagagataag tgctgaaagc
     2401 atctaagcac gaaacttgcc ccgagatgag ttctccctga ctccttgaga gtcctgaagg
     2461 aacgttgaag acgacgacgt tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta
     2521 accggtacta atgaaccgtg aggcttaacc ttacaacgcc gaaggtgttt tggcggatga
     2581 gagaaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
     2641 gaatttgcct ggcggccgta gcgcggtggt cccacctgac cccatgccga actcagaagt
     2701 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
//
LOCUS       DAJTOR010000285         2741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTOR010000285 DAJTOR010000000
VERSION     DAJTOR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.291"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..137
                     /locus_tag="OO249_004112"
                     /pseudo
     CDS             1..137
                     /locus_tag="OO249_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            387..1253
                     /gene="ospB"
                     /locus_tag="OO249_004113"
     CDS             387..1253
                     /gene="ospB"
                     /locus_tag="OO249_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5638192.1"
                     /translation="MNLDGVRPYCRTVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            1583..2323
                     /gene="phoN2"
                     /locus_tag="OO249_004114"
     CDS             1583..2323
                     /gene="phoN2"
                     /locus_tag="OO249_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5638193.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT          904 a          498 c          516 g          823 t
ORIGIN      
        1 cagcggtaag agaagttcat catacttggc gcagttacgc gatgtttctg ctagaggtga
       61 tgccgtaaag aacggcagaa cattaccgga acaagatagc ggtctacctg cactggtatc
      121 agacccgggg cttccccgga tgatctccac ggtactttag gtactgtgtc tatattatgt
      181 aggtaggatc aattaacttt gtagttgaaa ttctttcatt gaaataatga ataaaaaaga
      241 caaaccgcat taaacaatgc cagaatataa ttgcattttt gttccgtttt tttaaatcat
      301 agtatacaaa gaagcttgca tactatatga aaatataaca ccctttacta tgatgtttat
      361 aaaacaatat atggagtcat gtaggtatga atttagatgg tgttagacca tactgtagaa
      421 cagtcaataa aaagaatgaa agcatatcag atattgcatt tgcacatata ataaaaaggg
      481 taaaaaattc atcatgtact cacccaaaag cagcattggt ttttttagga gagaaaggtt
      541 tttgtgatag caatgatgtt ctatctatta tgggacaaca aataccaaga gtatttaaga
      601 acaagatgtt atatgattat gtttttaaaa atgaaaaaag taaaaatgat tttctaaaaa
      661 tggctgaatc atggctacca cagagtgaac caatagtaat aaataatgat gatgacgcat
      721 tgaatgctgc tgcttatttt tctgtaaaaa aagcgaaaat aaaaacagta aacgatactg
      781 attttaaaga gtataataag gtttatattc ttgggcacgg tagtcctggt tctcatcaat
      841 taggccttgg ttcggaactt attgatgtac aaacaatcat ttcaagaatg aaagactgtg
      901 gtattctaaa tgtgaaagat atccgtttta cttcatgcgg ctccgctgat aaagtggctc
      961 ctaaaaattt taacaatgcc cctgctgaaa gtctttcttg tatccttaac tctctgcctt
     1021 tttttaagga aaaagaatct ttgctagagc agataaaaaa acaccttgaa aacgatgagt
     1081 cattgagtga tggtctaaaa atatccggct atcatggata tggagttcac tatggtcaag
     1141 agctttttcc ctactcacat tatcgttcaa cttcaattcc tgctgatccg gagcatacag
     1201 taaaaagaag ctctcagaaa aagactttta ttattaataa agaactggat tagtataaaa
     1261 tttttaacct atagttaatt gtttgcattt aagatgaagt tatttttttc tttagtagaa
     1321 aagtgcttcc ctatgaggga aaatcacaaa tcatcataat acagagacaa aacgatacga
     1381 acaaatagat aaaaaacatc attcttttac tacattattt ttcgtgaata tgaaaaataa
     1441 tgttattact ttaatataag actatttttt gtttttccat cactctgttc aaatttttcc
     1501 gcatgacttg tgttttttgt aacacagctc gttttttaca gctgaccaaa atcatcaatt
     1561 aattatgcta aggaaataaa ttatgaaaac caaaaacttt cttctttttt gtattgctac
     1621 aaatatgatt tttatcccct cagcaaatgc tctgaaggca gaaggttttc tcactcaaca
     1681 aacttcacca gacagtttgt caatacttcc gccgcctccg gcagaggatt cagtagtatt
     1741 tcaggctgac aaagctcatt atgaattcgg ccgctcgctc cgggatgcta atcgtgtacg
     1801 tctcgctagc gaagatgcat actacgagaa ttttggtctt gcattttcag atgcttatgg
     1861 catggatatt tcaagggaaa ataccccaat cttatatcag ttgttaacac aagtactaca
     1921 ggatagccat gattacgccg tgcgtaacgc caaagaatat tataaaagag ttcgtccatt
     1981 cgttatttat aaagacgcaa cctgtacacc tgataaagat gagaacatgg ctatcactgg
     2041 ctcttatccc tctggtcatg catcctttgg ttgggcagta gcactgatac ttgcggagat
     2101 taatcctcaa cgtaaagcgg aaatacttcg acgtggatat gagtttggag aaagtcgggt
     2161 catctgcggt gcgcattggc aaagcgatgt agaggctggg cgtttaatgg gagcatcggt
     2221 tgttgcagta cttcataata cacctgaatt taccaaaagc cttagcgaag ccaaaaaaga
     2281 gtttgaagaa ttaaatactc ctaccaatga actgacccca taaagctgga cagcctgtat
     2341 caggctatgg agggcccata gacaaatcta ccctatatga gcataggagg agtctatggg
     2401 cacaccacgt tttacccctg aatttaaggg attactggaa aggctgggac atatccctcc
     2461 ggcagaagca gaaaaagctt attatgctgc catcggaaac gatgatctgg caacctgagt
     2521 tcacagataa aacattctct aggaaactcg gggcggttcc gttcaccaca tgcaatgtgg
     2581 tgttgcaggg gaacggtctg caccatcccc tatgtcgatc aatataacag aaatgacaac
     2641 ttcagattca gggcacaacc taaatatatt ttaggtcacc tctcaaatcg tttgcctgat
     2701 accgctccat tttttaataa aaaaatcaat catttttgaa t
//
LOCUS       DAJTOR010000286         2653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTOR010000286 DAJTOR010000000
VERSION     DAJTOR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.292"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            29..865
                     /locus_tag="OO249_004115"
     CDS             29..865
                     /locus_tag="OO249_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5638194.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSY
                     MWFETGDNGNEYFKWRSRQSTTTKDLMTLKWDALNILVNAVINGSLGVGSTNALGGSS
                     IVLGDNDTGFKQNGDGILDVYANSQRVFRFQNGVAIAFKNIQAGDGKKFTLSSSNNST
                     KNATFNLWGASTRPVVAELGNEAGWHFYSQRNTDNSVIFSVNGQIQPSNWGNFDSRYV
                     KDVRLGTRVVQLMARGGRYEKAGHAITGLRIIGEVDGDDEAIFRPIQKYINGTWYNVA
                     QV"
     gene            834..1397
                     /locus_tag="OO249_004116"
     CDS             834..1397
                     /locus_tag="OO249_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5638195.1"
                     /translation="MAHGITSHRCKLCSILKNIKSGNPKTKEQYQLTKNFDVIWLWSE
                     DGKNWYEEVNNFQDDTIKIVYDENNIIVAITKDAPTLNPEGFSVVEIPDITANRRADD
                     SGKWMFKDGTVVKRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMAMDEERT
                     RLESWERYSVLVSRVDTANPEWPQKPE"
     gene            complement(1426..1959)
                     /locus_tag="OO249_004117"
     CDS             complement(1426..1959)
                     /locus_tag="OO249_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5638196.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYYHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1962..2519)
                     /locus_tag="OO249_004118"
     CDS             complement(1962..2519)
                     /locus_tag="OO249_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5638197.1"
                     /translation="MAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWDSG
                     AYNALIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGVELGWSDFYTT
                     TRKPSAGDVGAYTRAECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGNRD
                     ELIDTTQARPIQYCINGTWYNAGSI"
BASE COUNT          758 a          568 c          590 g          737 t
ORIGIN      
        1 ggaaacggta aacagggctg gtaacgccgt gcaaaaaaat ggcgatacct tgtccggtgg
       61 gcttactttt gaaaatgact caatccttgc ctggattcga aatactgact gggcgaagat
      121 tggatttaaa aatgatgccg atggtgacac tgattcatac atgtggtttg aaacaggcga
      181 caacggcaat gaatatttca aatggagaag ccgccagagc accacaacaa aagacctgat
      241 gacgctgaaa tgggatgcac taaatattct tgttaatgct gtcattaatg gcagtcttgg
      301 agttggttcg acgaatgcgt taggtggtag ctctattgtt cttggtgata atgacaccgg
      361 atttaaacag aatggagacg gtattctgga tgtttatgct aatagtcagc gtgtattccg
      421 ctttcagaat ggagtggcta ttgcttttaa aaatattcag gccggggatg gtaaaaaatt
      481 cacgctatcc agctccaaca actccacaaa gaatgcaacg tttaatttat ggggtgcttc
      541 aacccgtcca gtagttgcag agttaggcaa tgaggcagga tggcatttct atagccagcg
      601 aaatacagat aactcggtaa tattttctgt taacggtcag atacagccca gcaactgggg
      661 gaattttgat tcacgctatg taaaagatgt tcgcctgggt acgcgtgttg ttcaattgat
      721 ggcgcgtggt ggtcgttatg aaaaagccgg acacgcaatt accggattaa gaatcattgg
      781 tgaagtagat ggcgatgatg aagccatctt cagaccaata caaaaataca tcaatggcac
      841 atggtataac gtcgcacagg tgtaaattat gcagcatatt aaaaaatatt aagtccggaa
      901 atccaaaaac aaaagaacaa tatcagctaa caaagaattt tgatgttatc tggttatggt
      961 ccgaagacgg taaaaactgg tatgaggaag taaataactt tcaggacgac accataaaga
     1021 ttgtatacga cgaaaataat attattgttg ccataaccaa agatgcccca acgcttaatc
     1081 ccgaaggctt tagtgtcgtt gagattccag atataacagc caatcgtcgt gccgatgatt
     1141 cagggaagtg gatgtttaag gacggaactg tggttaagcg gatttataca gcagacgaac
     1201 agcaacaaca ggccgaatca caaaaggtcg cgttgctttc tgaagctgaa agcgttattc
     1261 agccactgga acgcgctgtc aggctgaata tggcgatgga tgaggaacgc acacgactgg
     1321 agtcatggga acgctacagc gttctggtca gccgtgtgga tacagcaaat cccgaatggc
     1381 cacaaaagcc tgagtaaaaa ttaaggccca atatcgggcc ttctctcatt ctggttgttc
     1441 gggaaacgtt actggcaggc tggaggtgtc tgtagattcg actttctgcg catagagcat
     1501 ccactcggtt aatttttgtt tattctcgtc ggaaatgatg cccagccgta gctgtgagtc
     1561 ccatagctgg gttttatccc tgacaagttg caacaggctt tgcttttcat tttccgcttg
     1621 ttgcctctgc tcttcctcgg tataagttcg ctttatcact acgccatctt tgaacaacca
     1681 tttccccgaa atatcagccc ggcgatttgc tgtaatatca ggtaattcaa cgacgcttgc
     1741 gccttccgga ttaattgctg aaacatcctt ttcaatacaa ataataacgc cgttatggta
     1801 atagaccatt ttcaaagtgt ctggctggaa attcttttgt tcctcatacc agtttttccc
     1861 atcatctgaa taaagccatt tgatgttaaa ttgctttgtt agctggtatt gctcttttgt
     1921 tttagggttg ccagcagtaa tgttttttaa gtgcatcatc gttaaatact ccccgcgtta
     1981 taccacgtcc cattaatgca atactgaatt ggccttgcct gtgttgtatc aattaattca
     2041 tcacggtttc cgttaactga acccgtaacg acataacctg acctgtcaga ccagccggga
     2101 ccattccatg tctgaacaga tgacagaccg ccaaggcgaa tacctgtaat aaaccttgag
     2161 ttacattctg cccgcgtata tgcaccaaca tctcccgctg aaggttttcg tgtcgtggta
     2221 tagaaatctg accaacctaa ttcaacccca taaccatcac gagccgacct gtaggaaatg
     2281 ccaccatttt tataattcac acggaattgt acggcaggac agctaccagc atttatatta
     2341 aagtgaagaa ttaatgcaga tgcaccacca atcagggcgt tatatgcacc actatcccaa
     2401 ttccatccaa cggatgtatc accattaaca gtattacctg tttgtcggag tgcaaatgca
     2461 ttgacatttt tggcactaat tgaaatatcg ctagaaccat caaaaccaac tccagcaatt
     2521 tttcgtgctg ttttcagctt tgttgcagta gcggcattgc cggacagttc cccagaaagg
     2581 ccaccgctga atgtttgtcg attggtccag gtattcgctg tactgagtaa cggtattttc
     2641 tccccgcttg tgc
//
LOCUS       DAJTOR010000287         2643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTOR010000287 DAJTOR010000000
VERSION     DAJTOR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.294"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..2414)
                     /locus_tag="OO249_004119"
                     /pseudo
     CDS             complement(1..2414)
                     /locus_tag="OO249_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310260.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2414..2643)
                     /locus_tag="OO249_004120"
                     /pseudo
     CDS             complement(2414..2643)
                     /locus_tag="OO249_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT          607 a          666 c          654 g          716 t
ORIGIN      
        1 catagacatt cagctggctg ttgcctgcgg tgatgtcata ccccagtatc atgctggcac
       61 gccccagcag cgattcgtag tgattcagat gtatattgag gccattactc gccttcatag
      121 ccatctcatt ctggtggctg tatgtaagct gggtttgcgg ctctatatag aacccataac
      181 cagtagggga caggttgaac ctctgcccgg cttctaggga gatgctcatt ccattcgcag
      241 tgccgttggc gttaacgccg ttgttatgac tgtccagtac gtggaaacta tttttctggc
      301 gcgatgcttt tataacgaga tcgctgtaaa aaccgttttg tgccatgtaa ctggcgtaca
      361 ttcccatgta gtctgaacgt gcggtaccgt cgcctccgct atagtccggc gatgcatgtg
      421 ttgagccaat atacagaccg acatacaacg gcatttcatc agagagacgt ttatccccac
      481 caaactggat accgctgtaa cccatgtcaa agccgctcag tttgccactg gcaaaggagt
      541 ccaggcttcc cccataactg cgcaaccaga tattaccgtc tttactctga tttcgcaggt
      601 cacccatccg ttgcatcagc gtacggtttt caacatagtt caataagtaa ccgacattga
      661 gataattgcc gccagcatct gcggtcgtgg tgggtttagg cgtgggagcg ggttcaggcg
      721 taggatcggg gttgactatg ggaggctgag cgggagctgg tgtgggttca ggattaggag
      781 tcggttccgg aactgtcccg gaagcgtaaa gctcccagtt agtgccattt ttacgcacat
      841 cgtacagata tcccccccaa ctcaacctga gaagacgcgc tgaacgaggc cgcgccgtca
      901 gtggttttta ccactgtcag aacttcattt cccgttgtgg cctcgctgcc ctggttgcgg
      961 atagccaata cgtgattacc agcactactc ccgctgatat tcaataaatc ccctttatta
     1021 ttaacgccat taccctcgcc aacaacatca gcacgcataa taaaggtact gttaccgctc
     1081 aggttctcta cgtttaatgt ggcaaatgtg ccggcagttg acccgtggct ggcaaaatcg
     1141 acagttgaat ggctcagcgc cagcgtgtcg agattagaat tacttgttac gttccagacg
     1201 ctgttattca ttgcaacgtc cagttttccg ccattgacat tatcgctgag ggaggaacct
     1261 gtccaaaccg acccagggtg catatccaga ttgattagcc cacctttgga aaaaatgcta
     1321 ccgttgataa gcatacgacc cgaggcgttg atgcggctgg cggcataacc atcgtcatgt
     1381 tgcgttgcaa tggccatctt gtcgggtgta ctcatatcaa tgaccagatc gctcgtgagg
     1441 tcgatctggc tgttggtcat ggcataaatt cccctggctc ctgccgcgcc ggtgatagcc
     1501 aaactgtctc cggttattct accgccgctg agcgcccaca aacccagcgc cagactgcca
     1561 ttacgatcaa ctgtaatatc ggtattttgc atgttgacaa cagccgttgc cgtctgggcc
     1621 gaggcaccat aagaaccgcc ggaaaagatg ctgtttcgtt gcgctgccgt gccagaaaaa
     1681 ttgattgtcg cgtctgaacc actggtgacg aggccaccgc cctgcgcgga agaaatatgg
     1741 ctatctgcac cgatagtggt tgtaccacca cgaacttcga cgccattggc cgcggctcca
     1801 gttacatcaa cagtgagtgc attcgcgcta acctggccaa agctccagag gccgtgtgca
     1861 ttatcgccat tggttttaat ggaactgttt gttccgagat cgacaacaga gtttttctgt
     1921 acgtttatcc ccatggcgct gtagccctga acatcgattg tcaggtctgt agccgtaaaa
     1981 cgcgcagcac cattggcgtt attgccgttg agaccaccga tataaacacc tgtacttcca
     2041 tcggtcgtga ttttacttcc gcttccaaga tcgacactgg tgccatagtc attgatggtt
     2101 aggcctatac cgttcgagtt ttcaatggtg aattgagtgg ctgttagtgt tgagctatgc
     2161 ccaataatga tgccgtcatc gttactctta atggtgctgc ctgtgcctaa gtcagcatgg
     2221 gtatagtcac caattaagtt aatgccgatg gcagaggttt gaccaacaac atctactgtg
     2281 agtcggttag ctgtcaggga gctgtttttg ccctgaataa tgattccttt tgcatatcca
     2341 gaggcgtcgt tgacattgac ggtgacatca ttacccaggt tgatattgcc aggcgtgttt
     2401 cctgctgggg tcattacacc atagaggttt cctgatggat cggcggtatc tcctgtaatt
     2461 ctgtcaccat cattaatagt aatattggtg ccatattgac ttcctgtatc agcagctatc
     2521 agcagggggg aaaagataat tgcattcaaa ccaatagcgc caaattttat cagttgaatt
     2581 gagggcgaac ggatttttaa ttcagcgaga cgatctgctt tggtattccc attgtgttgt
     2641 ttg
//
LOCUS       DAJTOR010000288         2629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTOR010000288 DAJTOR010000000
VERSION     DAJTOR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.295"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(264..2354)
                     /gene="iha"
                     /locus_tag="OO249_004121"
     CDS             complement(264..2354)
                     /gene="iha"
                     /locus_tag="OO249_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HCR5638198.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          642 a          718 c          578 g          691 t
ORIGIN      
        1 catgaaagag ttgttgccct ggaacgtcac tctctctgta aactaatttt taccccacgt
       61 ccttcacggt gcgcttacac tctggacaag tatgtaaatg ataagttaaa aataatccgt
      121 gcatttcctg tgacgatatt agttgttggt aacgaaatct cattagcgga tcggaaatgc
      181 acttaaccta atacgttgcc actgttccgc caggagttgc agtggcaacg tattctaccg
      241 tcagtgatag cgttttgtta ttatcagaac tgatagttca gcgacatcca gtaatttcgc
      301 tcaggtatca catatcctgt tgttgatgat cccgtctgga agtaatcacc ggcatacagc
      361 gtactcttac cggcactgta caggctcacg tcactgtaat ccttgttgag caggttattc
      421 accgcagcat tcagcgtcag ggcatccgtc atcttccagg acagacctgc atccaccacc
      481 gtccaggctt tcaggtattc tcctttctca tcatacactt tcttctgtac agcgctcagt
      541 gacgaataat tctgggtgaa acgtggtgtt ttcccgcgat aacgggcacc cagccatgat
      601 gccacctctt cggtgatctg ccagttcagt ttcgcattca ccatgtgttc aggggtataa
      661 ctcagcggcg cacctttgtt atcaccatca cgttgttcac ttcgggtcca ggtgtaattc
      721 agtgacagcg tgacatcctc tgaccacagc ggcaatgtgc cggcaaattc cacaccgtgc
      781 aaccgggcct ttccgctgtt tacatagcta ttggtgttat catttatgga ataagagaca
      841 atcttgttgg agaagtcagt cataaaacct gtgacattgg cattcagacc ggcggggtta
      901 tcgtaataca ccccagcctc ataactgacg ctctcttccg gcttcaggtc ggggttacca
      961 agtagatttg tttttccctg cccggacaca ccactaatcc ctttatgtag ctgccccatt
     1021 ctgggtgcct tatatcccgt ggtcacaccg cctttcagcg tccaggcatc tgccacatcc
     1081 cagaccagat atgcacgcgg actgaagtgt cccccgaatt gctcatgatg ttcatagcgg
     1141 ctgcccgcag tcagcgcaag tgcatccgtg agatgccact catcctcagc aaataccgac
     1201 cagcttttct gccggaaagt ttcacctgtg ctggcaagga caactccgtc tttcatggac
     1261 gagctctgaa actcgccccc caccgtaacc agatgagatt ctcccagagg ggtaagcagt
     1321 aatgaattca ggataaggtt cgattcctta agctcccgcg gctgaccggc aagcccccat
     1381 ttgtcgcgct tcagtacact gcgtacgagc tcacgacctt tattttctgt ctcgttccag
     1441 ttcagatacg atttccatgt tccgaaggtg aaagtatgat catagccagc tgaaattttg
     1501 ttccgctcat agcgcagggt ccggtcatat ccccccgtca gactccccag ttgcccatcc
     1561 cggttatcat aacgctgccg ggtggtatcc atatcaaacc agagcacatc ctgctccgac
     1621 gccttccagt caagacgtgc accaagatta taattctgtg actccgtggg ataaggaata
     1681 cgcgtggctg ctgtatcgct cagtgatgtg accgatgagc cctgacgctg ttgtgtgcta
     1741 ccgcgtacct gcaggctgac agaatcatcc acaagggggc cactgctcca gaaattaaac
     1801 tggctgctgt taccccattt gttgctttcc tgcagattca gccctgcatt gacggaagag
     1861 agccatttgt ctgcattctt tctggtaatg atattcacca caccgcccat cgcatcagag
     1921 ccatacagtg tggacatcgg ccccctgata acctcaatac gctcaatggc ggccagaggg
     1981 ggcatgaacc cggtattcat ggcagaaaaa ccgttgggag tcacgtcact gcttccgccc
     2041 tgacgaacac catcaatcag tatcagcgtg taactggctg gcattcctcg gatgctgatt
     2101 tccagcccac cggttttacc cgtaccactt tcaacatcca caccctctac tgatctcaga
     2161 gcctccgcca gatcgtggta ctggctggac tgcaattcct cctggctaat cacagaaaca
     2221 ctggcggctg cgttagtcag ctttttctca tagccggatg ccgagacaat catcacatcc
     2281 tctgcagcag ctatgctgct ggctgaaaat ccgagacagg gaatgactac ggaagccaga
     2341 gtggttattc gcatattact aatctccgat catgttaacc ggcaagacaa tacacaaata
     2401 caaacaataa tgagaataat ttatatttgc cttttacaaa aaataaagat tattaacaaa
     2461 actaaatata aataacaact gcctcggcat gccagttaac agctatccgt atggcgtgtt
     2521 cggttttgcg aataaaaaat ggttactgta gaagcggatt tccaccacat gtcaaccatg
     2581 aatggtcagt ggtgcgagac gactgggatg atttggtcga tgacttctt
//
LOCUS       DAJTOR010000289         2626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTOR010000289 DAJTOR010000000
VERSION     DAJTOR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.296"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..2316
                     /locus_tag="OO249_004122"
     CDS             <1..2316
                     /locus_tag="OO249_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5638199.1"
                     /translation="KYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLLQPQFTVEQL
                     RELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQIALYRDARG
                     YVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPWRLRNYSTWT
                     RNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLASDDNMLPNS
                     QRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSGDLEVTIEES
                     DGTQRRFIQPYSSLPMMQRPRHLKYSATAGRYRADANSDSKDPEFAEATAIYGLNNTF
                     TLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGYQWRTQYIKD
                     IPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTIFDGVSLYAS
                     GSQQDYWGNNEKNRNISVGVSGLQWGIGYSLNYQYSRYTDENNDRALSLNLSIPLERW
                     LPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHNSSLNASYRS
                     PYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDANGASGVRIQN
                     YPGIATDPFGYAVIPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFN
                     ANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISSKSQSWTVRW
                     GNQADQQCQFAFNTPDSEPTTSVLQGTAQCH"
     gene            2327..>2626
                     /locus_tag="OO249_004123"
     CDS             2327..>2626
                     /locus_tag="OO249_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5638200.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL"
BASE COUNT          733 a          636 c          612 g          645 t
ORIGIN      
        1 aaatacgcac aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt
       61 tcacagaaaa aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg
      121 gtagaacaac tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa
      181 gatgacgata gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc tgctgaattt
      241 gatttcaatc atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca
      301 agaggttacg tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac
      361 tcgtttacag gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg
      421 aatatgcaaa atggtgctaa ttttggcccc tggcgattac gtaactattc tacgtggaca
      481 cgcaacgatc aggcgtcaag ctggaacact atcagtagtt atttacaacg tgatatcaag
      541 gcgttgaagt ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc
      601 tacaccttta ctggcgtgca actcgcttcc gacgataata tgttgccaaa cagccagcgc
      661 ggatttgccc caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa
      721 aatggttatg tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc
      781 tacccctctt ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacacaa
      841 cgtcgcttta tccagcctta ttcttcatta cccatgatgc agcgacctag gcatctaaaa
      901 tatagcgcga ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa ggaccccgaa
      961 tttgctgaag ccacggcaat atatggtttg aataatactt ttacgctgta tggcggcctg
     1021 ctcggttctg aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg
     1081 ggcgcgttgt cgatggatat caacagagct gacacccaat tcgataacca gcactctttt
     1141 catggctatc aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc
     1201 gctgttagct actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc
     1261 cgcaattggg actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca
     1321 atatttgatg gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac
     1381 gagaaaaaca ggaatatctc tgttggggtt tccggcctgc aatggggaat tggttacagc
     1441 ctgaattatc aatacagccg ctacactgat gaaaataatg accgtgcact ctctttgaat
     1501 ctcagtattc cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc
     1561 cagaaagatc gcccaaccca acatgaaatg cgtcttgatg gctcactgct ggatgatggt
     1621 cgcctgagct atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg
     1681 aacgccagtt accgttcacc ttatggaacc ttcagtgccg gatacagtta cggtaatgac
     1741 agcagccaat acaattacgg cgttaccggc ggcgtggtta tccatcctca tggcgtgacg
     1801 ctctcgcaat atctgggcaa cgcttttgcg cttatcgatg ctaacggggc ttctggcgtg
     1861 aggatacaaa actatccggg gattgctact gatccctttg gctatgcagt gattccttat
     1921 ctcacgactt atcaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc
     1981 gatcttgaac aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt
     2041 ttcaacgcca atatcggtta tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg
     2101 ttgccctttg gcgctcttgc cagcaacgat gatacggggc aacaaagtat cgtcgatgag
     2161 ggcggcatac tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg
     2221 ggaaatcagg cagatcaaca atgtcagttt gcttttaata caccggattc agaaccaaca
     2281 acctctgtat tacaaggcac agcgcagtgc cattaaggat aaaaaaatga aaagagcgcc
     2341 tcttataaca ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg
     2401 tggagccgac agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt
     2461 gaaccagaca gataacgtga caggacggga gtttacctct gcaacgctaa gtagcactaa
     2521 ctggcaatac gcctgttcct gctctgcggg taaggcagtt aaacttgtct atatggtcag
     2581 ccccgtactt accaccactg gacatcagac aggatattac aaactc
//
LOCUS       DAJTOR010000290         2593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTOR010000290 DAJTOR010000000
VERSION     DAJTOR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.297"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..997
                     /locus_tag="OO249_004124"
     CDS             <1..997
                     /locus_tag="OO249_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5638201.1"
                     /translation="ASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATT
                     NITWETFIPHIDFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVA
                     VCAILGSLAMGMMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQV
                     AALVFSIDAPLKVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVQVAILIMLPTLGI
                     GDMNNLYKWLLNLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIW
                     CFAFTAFACLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(998..1906)
                     /locus_tag="OO249_004125"
     CDS             complement(998..1906)
                     /locus_tag="OO249_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5638202.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINDARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQISQSQRHRPQ"
     gene            2006..>2593
                     /locus_tag="OO249_004126"
     CDS             2006..>2593
                     /locus_tag="OO249_004126"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCR5638203.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT          673 a          552 c          635 g          733 t
ORIGIN      
        1 tgcttcacgc ggtatgaaat cgctgaaaat cgtcggttct gtggcaggga ttgcgatgtt
       61 tgttatgtcg ctcctgtatg tggcgatggc ggtaaccgcg cctgcgatta ctgaagtgca
      121 tattgcgacc acaaacatta cctgggaaac gttcattcct catatcgact ttacctacat
      181 taccactatt tcaatgctgg ttttcgcggt tggcggagca gagaagattt ctccttacgt
      241 taatcaaacg cgcaacccag gaaaagaatt tccaaaaggg atgttatgcc tggcggtgat
      301 ggttgcggtt tgtgccattc tgggctcact ggcgatgggg atgatgtttg actcgcgtaa
      361 tatcccggat gacttaatga ctaacggtca atattacgcc tttcagaagc tgggcgagta
      421 ttacaacatg ggtaatactt taatggtgat ttacgccatt gcgaataccc tgggacaagt
      481 agctgcgttg gtattctcga ttgatgcccc gcttaaagtg ctattaggtg atgctgacag
      541 caaatatatt ccagccagtt tatgtcgtac caacgcttct ggtacgcccg ttaatggcta
      601 ttttctgacc ctggtgcagg tggcaattct gattatgctg ccgactctcg gcatcggtga
      661 tatgaacaat ctctataaat ggctgttgaa tcttaactcg gtagtgatgc cgctgcgtta
      721 tttatgggta tttgttgcat ttattgcagt cgttcgcctg gcgcagaaat ataaaccaga
      781 atatgtcttt attcgtaata agcctctggc aatgaccgtc gggatttggt gttttgcctt
      841 taccgccttt gcctgtttga cggggatctt cccgaaaatg gaagccttca ctgcagagtg
      901 gaccttccag ttggcgctga atgttgcaac gccgtttgtg ctggtaggat taggactgat
      961 tttcccgctg ctggcgcgta aagcgaatag taaataatta ttgtggtcgg tgacgctgac
     1021 tctggctgat ctgacccgtt tttacataat cgagaaatgc gcgcaagccg caggacatat
     1081 actggcggtt tggataatag atctggaagc ctggacgctc tgtgctccag tctcccagta
     1141 cacattctaa acgaccagta tcaaggtact ccttgatctg ttcatagagc agatacccga
     1201 taccagcacc catcaaaacg gcctccagtt cagcatcaac atcatccaga atgatatttc
     1261 ctgcgacagc aatttccagc tcctttgcaa attgccagtg gaaaggtttg ccgctgggat
     1321 aacgaaaaac aacacattga tgattaagca gatcatgagg atggcggggt ttgccatatc
     1381 ttgcaaagta ctctggcgtt gctgctacac ataatttcac tggcgggccg atcgccacgc
     1441 tgatcatatc tttttcgacg atgccactaa gtcgaacgcc agcgtcaaag ccctgttgga
     1501 cgatatcgac aagagagtcg tcagttgtga gctcgacttt aatatcagga tattcacgtg
     1561 tgaaaccaac cagtaaaggc attaaaaata tacgtgcagc tacccgggcg tcattgattt
     1621 tgagcgttcc tgtcggtgtc agacgaaaat cattcatctc atctaacatg atctgaattt
     1681 cgtcgaaagc tgggcgcaat cgttcataga gattagaacc cgcttccgtt aacgatacgc
     1741 ttcgggttgt ccgattgaat aagcgaattt taagacgttg ttccagtgtt ttaatactat
     1801 ggctaatggc ggacgaggat aagcctaact catcgcccgc tgcacgaaag ctttgattac
     1861 gggccacggc aaagaaagtg gcaaagtcag acatattcat ccgcattagt gaaaatcctt
     1921 cattaactca tcctgaatca tcacgtttac aggattatgc cgcctgcgta aagtgcgctc
     1981 cagaacttaa cgtggaggta aaattatgca gtctgaacgt atttatttgg tatgggccca
     2041 tcctcgtcat gattcattga ccgcacatat tgctgatgcg atccatcagc gggcaatgga
     2101 gcggaaaata caggtgacgg aactcgattt atatcggcgt aatttcaacc cagtgatgac
     2161 gccggaagat gaaccagact ggaagaatat ggataaacgt tattctccag aggttcatca
     2221 gctttattca gagctgcttg aacatgacac gttagtggtg gtttttcctc tctggtggta
     2281 cagcttcccg gcaatgctaa aaggatatat tgacagagta tggaataatg ggctggctta
     2341 tggagatggg cacaaattac cattcaataa agttcgttgg gtggcgctgg ttggaggaga
     2401 caaagaatca tttgtccaga tgggctggga aaaaaatata agcgattatt tgaaaaatat
     2461 gtgcagttat cttggtattg aagatgccga tgtcactttc ttgtgtaata cagtggtatt
     2521 cgatggggaa gaacttcatg cgagctatta tcagtcgtta ttatctcagg tacgggatat
     2581 ggtagatgca cta
//
LOCUS       DAJTOR010000291         2568 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTOR010000291 DAJTOR010000000
VERSION     DAJTOR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.298"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..356
                     /locus_tag="OO249_004127"
     CDS             <1..356
                     /locus_tag="OO249_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5638204.1"
                     /translation="KPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQKAG
                     IASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVS
                     RRDQESALACWDIDR"
     gene            512..694
                     /locus_tag="OO249_004128"
     CDS             512..694
                     /locus_tag="OO249_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638205.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            1021..1173
                     /locus_tag="OO249_004129"
     CDS             1021..1173
                     /locus_tag="OO249_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001228697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCR5638206.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPEWIMQPAPDWQTPLNGII
                     SPSESG"
     gene            1259..1483
                     /locus_tag="OO249_004130"
     CDS             1259..1483
                     /locus_tag="OO249_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638207.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            1828..2154
                     /locus_tag="OO249_004131"
     CDS             1828..2154
                     /locus_tag="OO249_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HCR5638208.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(2286..2486)
                     /locus_tag="OO249_004132"
     CDS             complement(2286..2486)
                     /locus_tag="OO249_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR5638209.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
BASE COUNT          634 a          556 c          694 g          684 t
ORIGIN      
        1 gtaaacctgt cgtcccgggc atgaagttgt cgaaggaaaa atgcgaccag gtcaacgcca
       61 ttgagcgtga taaggcgctg gcatgggtgg agcgcaatat taaagtgccg ctgaccgaac
      121 cccagaaagc gggtatcgcg tcattttgtc cctataacat tggccccggt aagtgtttcc
      181 cgtcgacgtt ttacagacgg attaatgctg gtgaccgcag gggagcatgc gaagcgattc
      241 gctggtggat taaggacggt ggcagagact gccgtatccg ctcaaataat tgctacggtc
      301 aggtatcccg gcgagaccag gaaagtgcgc tggcgtgctg ggacatcgac agataagcag
      361 aatattttgc tgaaaataag gcatgagcac gcggacggat aacacgaaat cctgcgaact
      421 ggcgaaacgt aagtgaataa aagtaaaaac cccgtttgtt ggcaccaggc ggggttttgt
      481 gtttctgacc ttgagtaagg caagggagaa catggcgaag tataaacgaa ttctgttgag
      541 gttgaccatg aaaaacggcc ttgaactgaa agcgcctgta actgatgaca tcagcagagc
      601 actggctttt gccattaagt gggtggcggt cggtgttgct gtgtccccga tgctgtatgg
      661 gctggcaaaa ctggtcattg cgttgaaatc gtgaagggag gattaagcat gtcagacaaa
      721 ctcataacgc tggcgaagat cctctgtgta attgtcggca tttcattttc actaatgctg
      781 gttgctcttt ttctttccat ggcctggatg atgttgtctt cgtcggggct gctggggtga
      841 acataaaccg aatgctttcc gcgtttaccg ttattctgct ggtggtctgt ggtgcgctgt
      901 ggctggcaac agaccattac cgtgataacg ccatcaccta caaagcgcag cgcgataaaa
      961 aagccagaga gctggagctg gcaaacgcaa ccattactga catgcagcag cgccagcgtg
     1021 atgttgccgc tggtcgtaag cgcctgcgga tcaacgccac ctgctccggt accgtgcgtg
     1081 aagccaccgg cacctccgga atggataatg caaccggccc ccgactggca gacaccgctg
     1141 aacgggatta tttcaccctc agagagcggt tgatgacaat gcagaagcaa ctggaagggg
     1201 cgcaggaata tatccgcact cagtgtatta actagtattt ttgttatccg gagaatgcat
     1261 gaagaaatta ctggtaaccg taaagccttt tcagggaaca attccgttcc gtattttgca
     1321 gcgtggtcgt gttcttgttg aaggttcgtt cagtggtaaa tgtacgcaat tacactcccg
     1381 gacctttcag gtgaatgcca cgaatgaaga gctaaccgtt gagtgtacga tgaatgccgc
     1441 taaatgccgc atggtatccg ctgcattaca gccagtgtgt tgagcgacct tattaaccat
     1501 gcgcggtatt gtcgccgtat ccccgcatta acagagaccg cagcccgacc gggagactcc
     1561 tctgcgcgag tgtgcgggga taatcaaaaa cgatacacac cggggtttac cgcgttaacg
     1621 gagcgcggcg ttgtcccctc atggtcgctg gtccggtgcg atggtggaag aaaccggacg
     1681 atgtgttacc tcgcaagctc tgttatgtca tgtgtctgat ttgtgattta agtcggataa
     1741 ttgtcgttgc cattaagcag aggattgatg accgacaggg tggcattgtt agaataagac
     1801 ttattcttat ctgtgccggg aatgaaaatg aaaagaaacc ttccgttaat tattttgttg
     1861 tcttctctgg ttatgggctg tacgcaacat aaaacagata tgccccgaca gttggttaag
     1921 gcattaccac aatatccggc ctatgcagcg gcaaattata taaagggacg ggttgatgtg
     1981 aagtttgata ttggtgctga tggtactgtc acccgaattg agtttatccg ttcagagccg
     2041 caccatttgt ttgatgagca ggttgtaaag gcgatggcaa aatggcgatt tgagaaggac
     2101 aggccgtgta aaggcgtgaa gaaaacgttt atctttagtc cttctgcacc ctgattattt
     2161 catcagaaat taattatcac tctgttgtta ttctgtacat cccggcaggg taagtcttgt
     2221 tccgtcggat atgaagatga aatattgttg gaggacagtg ggtacctgct cctgtaaccg
     2281 aacgttcatt tctcgttatt tttcatgctg gccgggcgca gatgcgttgc atctgttgcc
     2341 agccttctcc tgcaggcttc aataacccac gctgaaaagt ttcctgaacc tttcagatca
     2401 agagcgatgt taatttgttc aatcatctgg tttggaaatc ggatgttgcg ggttgttgtt
     2461 ctgcgggttc tgttctttga tgacataatg tttccccata ttcagtgttg ctgatttgta
     2521 ttatctgaag ttgcttttac gttaatttga tgcagatcaa tcaatacg
//
LOCUS       DAJTOR010000292         2508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTOR010000292 DAJTOR010000000
VERSION     DAJTOR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.299"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..2290
                     /gene="ppsA"
                     /locus_tag="OO249_004133"
     CDS             <1..2290
                     /gene="ppsA"
                     /locus_tag="OO249_004133"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5638210.1"
                     /translation="LGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLD
                     KTDIDDVTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDENASFAVRSSATA
                     EDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNGRAISYRVHQGYDHRGVALSA
                     GVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDEFYVHKPTL
                     AANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQA
                     VQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIAEGRA
                     IGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASAIVTNRGGRTCHA
                     AIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMP
                     DLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQ
                     NEIREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERY
                     EPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQA
                     KAVVEELARQGLKRGENGLKIIMMCEITSNALLAEQFLEYFDGFSIGSNDMTQLALGL
                     DRDSGVVSELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGI
                     DSLSLNPDTVVQTWLSLAELKK"
BASE COUNT          572 a          634 c          715 g          587 t
ORIGIN      
        1 cctgggtgaa atgattacta acctttccgg aatgggcgtt tccgttccga atggtttcgc
       61 cacaaccgcc gacgcgttta accagtttct ggaccaaagc ggcgtaaacc agcgcattta
      121 tgaactgctg gataaaacgg atattgacga tgttactcag cttgcgaaag cgggcgcgca
      181 aatccgccag tggattatcg acactccctt ccagcctgag ctggaaaacg ccatccgcga
      241 agcctatgct cagctttccg ccgatgacga aaacgcctct tttgcggtgc gctcctccgc
      301 caccgcagaa gatatgccgg acgcttcctt tgccggtcag caggaaacct tcctcaacgt
      361 tcaggggttt gacgccgttc tcgtggcagt gaaacatgta tttgcttctc tgtttaacgg
      421 tcgcgccatc tcttatcgtg tgcaccaggg ttacgatcac cgtggcgtgg cgctctccgc
      481 cggtgttcag cggatggtgc gctccgacct cgcatcttct ggcgtgatgt tctccattga
      541 taccgaatcc ggctttgacc aggtggtgtt tatcacttcc gcatggggcc ttggtgagat
      601 ggtcgtgcag ggggcggtta acccggatga gttttacgtg cataaaccga cactggcggc
      661 gaatcgcccg gctatcgtgc gccgcaccat ggggtcgaaa aaaatccgca tggtttacgc
      721 gccgacccag gagcacggca agcaggttaa aatcgaagac gtaccgcagg aacagcgtga
      781 catcttctcg ctgaccaacg aagaagtgca ggaactggca aaacaggccg tacaaattga
      841 gaaacactac ggtcgcccga tggatattga gtgggcgaaa gatggccaca ccggcaaact
      901 gttcattgtg caggcgcgtc cggaaaccgt gcgctcacgc ggtcaggtca tggagcgtta
      961 tacgctgcat tcacagggta agattatcgc cgaaggccgt gctatcggtc atcgcatcgg
     1021 tgcgggtccg gtgaaagtca tccatgacat cagcgaaatg aaccgcatcg aacctggtga
     1081 cgtgctggtc actgacatga ccgacccgga ctgggaaccg atcatgaaga aagcatctgc
     1141 catagtcacc aaccgtggcg gtcgtacctg tcacgcggcg atcatcgctc gtgaactggg
     1201 tattccggcg gtcgtgggct gtggtgatgc aacagaacgg atgaaagacg gtgagaacgt
     1261 cactgtttct tgtgccgaag gtgataccgg ttacgtctat gcggagttgc tggaatttag
     1321 cgtgaaaagc tccagcgtag aaaccatgcc ggatctgccg ttgaaagtga tgatgaacgt
     1381 cggtaacccg gatcgagctt tcgacttcgc ctgtctgccg aacgaaggcg tgggacttgc
     1441 gcgtctggaa tttatcatca accgtatgat tggcgtccac ccacgcgcac tgcttgagtt
     1501 tgacgatcag gaaccgcagt tgcaaaacga aatccgcgag atgatgaaag gttttgattc
     1561 tccgcgtgaa ttttacgttg gtcgtctgac tgaagggatc gcgacgctgg gtgccgcgtt
     1621 ttatccgaag cgcgtcattg tccgtctctc tgattttaaa tcgaacgaat atgccaacct
     1681 ggtcggtggt gagcgttacg agccagatga agagaacccg atgctcggct tccgtggcgc
     1741 gggccgctat gtttccgaca gcttccgcga ctgcttcgcg ctggagtgtg aagcagtgaa
     1801 acgtgtgcgc aacgacatgg ggctgaccaa cgttgagatc atgatcccgt tcgtgcgtac
     1861 cgtagaccag gcgaaagcgg tggttgaaga actggcgcgt caggggctga agcgtggtga
     1921 gaacgggctg aaaatcatca tgatgtgtga aatcacgtcc aacgccttgc tggccgagca
     1981 gttcctcgaa tatttcgacg gcttctcaat tggctcaaac gacatgacgc agttggcgct
     2041 cggtctggat cgtgactccg gcgtggtgtc tgaactgttc gatgagcgca acgatgcggt
     2101 gaaagcattg ctgtcgatgg ctatccgtgc cgcgaagaaa cagggcaaat atgtcgggat
     2161 ttgcggtcag ggtccgtccg accacgaaga ttttgccgca tggttgatgg aagaggggat
     2221 cgatagcctg tctctgaatc cggacaccgt ggtgcaaacc tggttaagcc tggctgaact
     2281 gaagaaataa aataaatacc cggcggcgtt ttgtcgccgg gtttatgtga tccccgaaga
     2341 tgaaacttat tcaatctctt cacagacatc ctgcgttaat cgccgcataa tatcttttct
     2401 taacaaaaac ttttgtattt tacctgaggc agtgcgcggt agtttttcga ttaccacgat
     2461 atgttcagga tatttatatt ttgcgacccg tttacggcta aaaaaagc
//
LOCUS       DAJTOR010000293         2467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTOR010000293 DAJTOR010000000
VERSION     DAJTOR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.300"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(168..482)
                     /gene="fliE"
                     /locus_tag="OO249_004134"
     CDS             complement(168..482)
                     /gene="fliE"
                     /locus_tag="OO249_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5638211.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            697..2355
                     /gene="fliF"
                     /locus_tag="OO249_004135"
     CDS             697..2355
                     /gene="fliF"
                     /locus_tag="OO249_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR5638212.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     LSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2348..>2467
                     /gene="fliG"
                     /locus_tag="OO249_004136"
     CDS             2348..>2467
                     /gene="fliG"
                     /locus_tag="OO249_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="One of three proteins involved in switching the
                     direction of the flagellar rotation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5638213.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAA"
BASE COUNT          630 a          647 c          669 g          521 t
ORIGIN      
        1 cttcctatga gcaaagcgaa gacaaaacat gttatcgcgt ttacggatcg tggatggcag
       61 aaaactttca ggatcaggta tccattttaa accagaaata gggtgagttt gtcccatcca
      121 tgccccacgc gggcagatgg gatgtaatta ccaacaggtt acaaaaccta cacctgcatg
      181 gacatcactt cctgatacgc cgccaccagc ttattacgca cctgaatccc catttgcata
      241 gaaactgagg ctttttgcat atcggtcatc acatcgttta acgccacgcc gggttcaccg
      301 agggtgaatt tttctgcctg cgtgcgggcg gctgtttgtg tatcgcttat gcgatcgagc
      361 gcggcgtgca gctgcccggc aaaactaatg gtcggttgcg gcagtgaatc ctgcgcacgc
      421 gcactcatcg ccgtagcctg taactggctg ataacccctt caatcccctg tatcgctgac
      481 attttcgtct cctggataat ttctggtagc aaagcctacc agtaagtcaa taagacaaag
      541 gcgctaaata gcaacaaaaa aacgggttta ttggcggata gaaaaaaacg aaagcacaaa
      601 taatggcagc gtcaattttt cgagtttgct gacccgggag tgagtcttgt tccactttgc
      661 caataacgcc gtccataatc agccacgagg tgcgcgatga atgcgactgc agcccagaca
      721 aaatctcttg agtggcttaa tcgtctgcgt gcgaatccga aaattccatt gattgttgcc
      781 ggttccgcgg cagtggcggt catggtcgca ctgatcctgt gggcgaaagc ccccgactac
      841 cgcacattat tcagcaatct ttccgatcag gatggtggcg caattgtcag ccaactgacg
      901 caaatgaata ttccttaccg cttcagcgaa gccagcggcg ctattgaagt tccggcagat
      961 aaagttcacg agctgcgtct gcgcctggcg caacaaggtt tgcccaaagg cggcgcggtc
     1021 ggtttcgaac tactagatca ggaaaagttt ggtatcagcc agttcagcga acaggtgaat
     1081 tatcagcggg cgctggaagg cgagctttct cgtaccatcg aaactatcgg cccggtaaaa
     1141 ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc
     1201 ccttctgcat cggtgacggt aaatctatta cccggccgcg cactcgatga agggcaaatt
     1261 agcgccattg tacatctggt ttccagcgcg gttgctggtc tgccgccggg gaacgtcacg
     1321 ctggtggatc agggtggaca tctgttaacc ctgtccaata ccagcgggcg cgatcttaat
     1381 gacgctcagt tgaaatatgc cagcgatgtc gaaggccgga ttcagcggcg tattgaagcg
     1441 atcctgtcgc caattgttgg taacggtaat attcacgccc aggtcacggc gcaactggac
     1501 ttcgccagta aagaacaaac ggaagaacag tatcgcccta acggtgatga atctcatgcg
     1561 gcgcttcgtt cacgccagct taatgagagc gagcaaagcg gttccggtta tccgggcggc
     1621 gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata acgcgccaat cagcacgcct
     1681 ccggcaaatc aaaataaccg ccagcagcag gcgagcacca ccagcaatag cgggccgcgt
     1741 agcacacagc ggaatgaaac cagtaactac gaagtcgatc gcaccattcg tcataccaaa
     1801 atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag tggtaaatta caaaaccttg
     1861 ccagacggta aaccattgcc tctcagcaac gaacagatga agcaaattga agatctgacc
     1921 cgcgaggcga tgggcttttc tgaaaaacgc ggtgactcgc tcaatgtcgt taactcgccg
     1981 ttcaatagca gtgacgaaag cggcggagaa ctgccgttct ggcaacagca agcgtttatc
     2041 gatcagttgc tggctgccgg tcgctggttg ctggtactgc tggtggcgtg gctgctgtgg
     2101 cggaaagcgg tacgcccgca gttaacaagt cgcgccgagg cgatgaaagc tgtacagcaa
     2161 caggcgcagg cccgtgagga agtggaagat gcggtggaag tccgcctgag caaagacgaa
     2221 caactccaac aacgacgcgc taaccaacgt ctgggggcag aagtcatgag ccagcgtatc
     2281 cgtgaaatgt ctgataacga tccgcgcgtg gtggcgctgg tcattcgcca gtggataaat
     2341 aacgatcatg agtaacctga caggcaccga taaaagcgtc atcctgctga tgaccattgg
     2401 cgaagaccgg gcggcagagg tgttcaagca cctctcccag cgcgaagtgc aaaccctgag
     2461 cgctgca
//
LOCUS       DAJTOR010000294         2451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTOR010000294 DAJTOR010000000
VERSION     DAJTOR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.301"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            545..1084
                     /locus_tag="OO249_004137"
     CDS             545..1084
                     /locus_tag="OO249_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5638214.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(1310..1633)
                     /locus_tag="OO249_004138"
     CDS             complement(1310..1633)
                     /locus_tag="OO249_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR5638215.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1917..2111
                     /locus_tag="OO249_004139"
     CDS             1917..2111
                     /locus_tag="OO249_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5638216.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
BASE COUNT          834 a          577 c          512 g          528 t
ORIGIN      
        1 ttatcgccac tggcagcagc cactgataac cgaattagag gagtaagact tgaagtcatg
       61 cgcctgttaa ggctaaactg aaagaacaag ttttggtgac tgcgctcctc caagccagtt
      121 acctcggctc aaagagttgg tatctctgag aaccttcgaa aaaccgcctt gcagggcggt
      181 tttttcgttt acaggcagta gattacgcgc agaaaaaaag gatctcaaga agaaccttta
      241 attttttttc acacttggta tcctacttta acccataaaa taaatactta caccgacacg
      301 cccttaaaca aaagaaaaaa aaacaataaa aaacaccgca aaaatggatt gttaacaaat
      361 gaaaatactc aattcgaatc tcataacaaa catatcatca acaataagaa atcaccttct
      421 aacatcatcc aaaaatataa aaacacaccc atcaccaaac atggacgtaa caagtcatcg
      481 tcgtttattc cagaaagacg gaataggatt gagccaaaca ggaaaagcaa cacaacttgt
      541 cattatgtcg catggtggat ggaaagagat ctcccatcca caaacactat tcacgcgtca
      601 aaaaggcgat ggatggactg tagtccccaa aaacctaaga attgattttt atacaaaaga
      661 caacgacttc acaaagggac taagcgttct ttcagaggta aacaaacgcc atactgaagc
      721 tcaaaaaggg ttaacaccat cactaaacat aagcaaagat gacctaaaag ttctagcgaa
      781 cgcgcgcaat atacctcaat caaaattaga agaagaaatg atgtcacaag ccatatatag
      841 aaaagaatac gcaacaagta atgagaagat aaaaaactat gctctatatt accatgaaca
      901 agcccaaaat attatacaaa gacatcaaga tggcttagga aacaaaaata ttgatatagc
      961 atttataact gataaaaatc ataagaagca cctttctgac atatttaaag taataaatga
     1021 atcaggagta aaatatgatg taattcactt tggcgcttgc cgagtaaata gagaggagaa
     1081 ataatcatca tttaccataa tcccttaatt gtacgcacca ctgaaacgcg ctgaacgcga
     1141 ttctgacgca gacaccacaa aaatagatgt tttaccccgg aaaaacagga aggatgcgtc
     1201 aggatcgttt tcagctcgct gcatagctat gcatgaaagc gaatggagat cactttggga
     1261 gcttaccgtg ttcataccgt ctgttttcaa cagttttctc cccgggaagc taatctgcca
     1321 taagcctgga taacagggca cggtgatact ccgtaatagc gatcagcacc tccgcatact
     1381 ccctgtcccc gatacgcttg ccgtcgataa agagtttttt ctgtaacgcc cccagtctca
     1441 taacttcttt cagtacccga tcatcagtca gtgagttaac gttcttaccg agagctgccg
     1501 cccgtaaaaa accggaaacg gtcttgccgc attcagcagc cctttttctg atttcctggt
     1561 cttcttctgc cgtcagacga acagggcgac tgatagcccg cctgcgcgta ttacttccac
     1621 ttcgttttgt cataaaacat ggctccgtat ctgacatggg tgtcggggca aagccctgac
     1681 cagggcaatt gtaatagcgt gcatgtatgc gcggtataac aattgcacat cctgtcctgt
     1741 cagcaagctc gaattaccga caaaggcacg gcagtttttt ggcagttaca gtgttgtctg
     1801 acagaaatgc ccgtcaaaaa gccaggcggc agaatagcgg cagcaaaaca gtcgaaaagt
     1861 ggtacgcgat ataatcacat aacgcagatg acagacagaa cggagaaaac gaaaagatga
     1921 acaagcagca gcaagccgta ctcaacatgg cgggattcat aaaaagccag agcctgacgc
     1981 tgctcgaaaa actggacgca ctcgatgctg acgagcaggc caccatgtgt gagaaactac
     2041 acgaactcgc agaagaactc cagaacagca tacagacacg ctttgaagta gaaaatagca
     2101 cagagagata acgctcagga caggatgtcg aacggcacaa cagagcgtaa ggagaaaatg
     2161 tcacataagg cgctcttccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
     2221 gcggcgagcg gtatcagctc actcaaaggc ggtaatgcgg ttatccacag aatcagggga
     2281 taacgccgga aagaacatgt gagcaaaaag ccgaaggcag ggacaaaggc cgcgcaggtg
     2341 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2401 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg a
//
LOCUS       DAJTOR010000295         2441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTOR010000295 DAJTOR010000000
VERSION     DAJTOR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.302"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(26..2215)
                     /locus_tag="OO249_004140"
     CDS             complement(26..2215)
                     /locus_tag="OO249_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCR5638217.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
BASE COUNT          707 a          629 c          567 g          538 t
ORIGIN      
        1 taattccccg ttttttttac gcctgttaat cagctaatgg ctgcgggcga ccaatcaaat
       61 acccttgcag atattgcacc ccgagcttat gcaatagcgc ctgctgctgt tgcgtctcga
      121 caaactccgc gaccacactc aatgacttcg ctttcgccag atcggtaatt gatctcacaa
      181 tcatcgcatc cagcgtgttc gtgacaatat ctttcacaaa gacgccatca attttgatga
      241 tatcagcctg caaacgcttt aaccgttcgt agttggcata gccggtgcca aagtcatcaa
      301 tcgcaatccg gaaaccaaac ttatgcagct gctcgatgtt gtacatgctg ctttctgcgt
      361 tagaaaacgc ctgctcctcg gtgatctcaa gaatgaccgc ctgcggggag atgtgataac
      421 gcttaaacag acgaataatc cgcccggcaa tattcttttg cagcagcgtg agcggcatta
      481 aattgactga aaagcgcggt ccttttttgt cgcaagggtg tgttgccagc cacttcaaca
      541 aggattccag cacttgcaaa tcaaaacgcg cgctaaggtt aaactgagca ataaggggca
      601 gaaacttatc cggggtcata atgccgccgt catatttcag tcgcgcgagg atctcatcat
      661 aaccttcacc ctctttgttg cgaattggct gggcgtagag cagcaaatca ccctgatcta
      721 acgccgtgcg aatggtattc agcaatagca cctgtttagt ggtctgcccg gaaaccatct
      781 cctctctgct atccagcgcc agcacatgat gatgtgcgca ggattgctcc gccagccagc
      841 ttaactgccc caacaagggt tgcagggttt cctgatttcc atcaaaacgc ccccaggcag
      901 caccatagcc catatccagc ccggtattgt tccagtgaat ttgccgacta ttcaggatgt
      961 taaccatatg ctggagtcgc ccttccgttt ccggcccgct cagcactaac agcagttcac
     1021 tacccggcaa ttgatacaac ttttcgtttt cctgcatcaa cggcagcagc gtacggcaaa
     1081 ttgagcggat acagtgaacg cgcatcatta agccgtaatg acgactcata aactcaagat
     1141 tatcaatgcg caggcagcaa aaactcttgc ccgcctcttg ctccggcgct tgttccaacg
     1201 cacggaagtt gggcaggagc gttaacggat cggtcagcgc ctgcaaatgc cagcggcgat
     1261 taagccattc actgcgatga taaatgcgca ccatatagag caggcaaacg ctaaaggaaa
     1321 tcagcaccgc aagaataaat gccagcgaat attcggtttc caccccttgc aaaaagttct
     1381 ggttgtaatt cagaaggcaa agcgttgaaa cagcccaggt gagatttaaa aacggatagc
     1441 gaagcttacc gaccccgagg gtaaagatga tgaagaaaac gggtaccagg taaccggcaa
     1501 taaagtcgtt ttcataaggt gtgcacaaca gaagtagaag cacgcttaac gctgccagcc
     1561 agcttaaggt aaatgcgcgt ttctctttgc ccaacgacgg cgcgatatcc ctgcgccaca
     1621 atatctgcgc aaagtgggga cttacaatca tgcgagtgag atagtagaaa agcatgttgt
     1681 aaatcagcac agcggtgaaa aggcttagca aatcaacgac cgtgaaaatg gcatccgcat
     1741 cgccgaaaaa ggtggatatc tttagcggga aatcaaagaa gctccccaca agatacatgc
     1801 tgcatttgat gccgatcggc gtcaccaaac caagccagaa cagacgttgc cagacatatc
     1861 ggctggtcaa tccataacgc caacgcgtcc ccagctgcca gcgtaatatt gcgcaggcac
     1921 acaacacgac aaacgtctgg ctaccgagca tgaccgcagt ctgtaaaaaa gagagatgaa
     1981 agttccacag attggtgcaa aacatcccgc ataaaacggg aacgacacct cgccagccaa
     2041 agataaacaa gattgacagc attacgcaca gtggcatcca cgccagaaag atataactgg
     2101 aatggataat ggcaagtggc gaaataaaac gggatagctg aataagtacc acggtgagcg
     2161 taaacgctag tgtgaatatc ttgatatttt ttatcaggtt atgctccaca aacatgagta
     2221 cactactctc actgatcgga gttttcaggc acacaaataa taagtttatt cgcatcgcga
     2281 agcaaacagg gggccagcct gttgccgatt tttcatccaa tgatgctttc accaggcggc
     2341 actaacccgg acgtagactg tattacaaaa gcggcaaaaa gcagagacaa aaaacccccg
     2401 ctttgcagcg agggttggaa atttggtgga gctaagcggg a
//
LOCUS       DAJTOR010000296         2403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTOR010000296 DAJTOR010000000
VERSION     DAJTOR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.303"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..449
                     /gene="yeeE"
                     /locus_tag="OO249_004141"
     CDS             <1..449
                     /gene="yeeE"
                     /locus_tag="OO249_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5638218.1"
                     /translation="VLIGLIALLAWPLSEATGRMFGLGITSPTANILQFLVAGDVKYI
                     NWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGGCSIGN
                     GLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            463..690
                     /locus_tag="OO249_004142"
     CDS             463..690
                     /locus_tag="OO249_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5638219.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(733..2157)
                     /gene="sbcB"
                     /locus_tag="OO249_004143"
     CDS             complement(733..2157)
                     /gene="sbcB"
                     /locus_tag="OO249_004143"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5638220.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMPPLVHVSGMFGAWSGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPTDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          580 a          573 c          616 g          634 t
ORIGIN      
        1 ctgtactcat cggtttgatt gcgcttttag cctggcccct gagtgaagca accggacgca
       61 tgtttgggtt aggaatcact tccccaacgg ccaatattct gcaatttctg gtcgcgggtg
      121 acgtgaaata cattaactgg ggcgttttcc tggtgttagg gatcttcgtg gggtcattta
      181 ttgcagccaa agcgagccgt gagttccgcg ttcgcgcagc tgatgcacaa acaacattac
      241 gtagcgggtt aggtggtgta ctgatgggct tcggtgccag tattgcaggt ggttgctcta
      301 tcggtaatgg actggttatg actgcaatga tgacctggca gggctggatt ggccttgtat
      361 ttatgattct cggagtctgg actgcatcct ggcttgtgta tgttcgaccg cagcgtaagg
      421 cgcgactggc gactgctgcg gcaaattaag taaggtgtaa agatggcgat taaaaagctg
      481 gatgtggtga cgcaggtctg tccgtttcca ctcatagaag caaaagcagc actggcagaa
      541 atggccagcg gcgatgagtt ggtaattgag tttgattgca cccaggcaac tgaagcaatc
      601 ccacagtggg cggcagaaga aggacatgcc atcaccgatt atcagcagat tggcgatgca
      661 gcgtggagca tcaccgttca aaaagcctga ctcaacattg tcctccgccg taccagcggc
      721 ggaggcttca aattagacga tctcttccgc gtactgccaa agtgctttta acagcgccac
      781 tttttctttg tcatcggcat attgttgtac cagcatctgc aattcatcag cataaccctg
      841 caaaaactct ggcgtgaata cctggcggcg gtgctccagc cagcgttgct gctcggcata
      901 atccagcgtc cccgggaagt tgcgtgcccg ataattgaac aacagctttt caatccgttt
      961 atcaacaaaa gtgatatcca gtgccggtaa attacgtggc tcggtttcca gcacaatttt
     1021 cattgctgca cgatctgcgt cactgaaaaa gccgttataa agctgtgcat ccacgttatc
     1081 tgtaggcgta aacggttcgg cttccgcgaa tatcgccacc actttttcac gcacttgcgg
     1141 attttcacgc agaattttca ggttatcgag gcaatgctga cgattaattc ccagtcggtc
     1201 ggcatcttcc gggcgtagcg tattcgcctg ggccagcacc ggacatttat tgatgtgcac
     1261 cagcttaacc ggaacggcgg cgttatcgcc aagatcggtt tttgcggtat ataaacgctc
     1321 gcgcaatgtg tcgctatcca gttccagtaa tggcgaaatg tctcctgcca aatccaccat
     1381 aattacagca ttgcgatttt caggatgcca cgccagcggt gccacccagc tggtattgcc
     1441 gctccatgcc ccaaacattc cggaaacgtg aaccaggggg ggcatctgcg gaacatcaat
     1501 caacgccatc agtttgtgtt tattacgatg ggtaaagaga taatcaaaca ggcgtggctg
     1561 acgcgttttt accagtttcg ccatcgcaat agtggcgtac acatcagcca tcgcatcgtg
     1621 ggcgttgcta tgttcaatac cattcgcttt ggttaaatgc tcaaggcgaa agctcggtag
     1681 accgtcatca ttttcaggcc agtttattcc ttccgggcgc agggcataac aggcacgcat
     1741 aacatccaac aaatcccagc gcgagttatc atgctgccag ctccaggcgt aaggatcgta
     1801 gaaattacga taaaaaacgt tgcgtgtgac ttcgtcgtcg aaacgcacat tgttgtagcc
     1861 cagaatacag gtcttcggta cggtaaaaag cgagtgaata cgggcggcaa acgcggcttc
     1921 gttttctcct ttcgcccgtg cttcctgcgg ggtaataccg gtaattaata ctgctccagg
     1981 ctggggtaaa tagtcatccg cgggcttgca gtaaaagact tcgggttcgc cgatgacatt
     2041 gaattcgcta tcggtgcgaa tggctgcgaa ctgtgcaggg cgatctaacg cggggtgcgt
     2101 gccaaaggtt tcgtaatcgt gaaagaggaa ggtaggttgt ttatctgtgt ctgtcattgt
     2161 ctagcattgt ccactgtagt taattaactg tctgttataa ctcgaaaaac aaccattttt
     2221 tcacctgagt atgtctacga acgtcaaata tagttgttta ctgtgcgctg ttgtccatgt
     2281 gatattgtgt accagatgtg taccaaacca ttatttctga gcgtaccaaa tattatttat
     2341 ggctataagc gacacaaaac tgcgtagtat ttatggtaaa ccatattcgg gcccacaaga
     2401 agt
//
LOCUS       DAJTOR010000297         2381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTOR010000297 DAJTOR010000000
VERSION     DAJTOR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.304"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..303)
                     /locus_tag="OO249_004144"
     CDS             complement(<1..303)
                     /locus_tag="OO249_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="catalyzes the transfer of alpha-xylosyl residue;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5638221.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYA"
     gene            complement(313..1695)
                     /locus_tag="OO249_004145"
     CDS             complement(313..1695)
                     /locus_tag="OO249_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR5638222.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1988..2082
                     /locus_tag="OO249_004146"
     tRNA            1988..2082
                     /locus_tag="OO249_004146"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2022..2024,aa:Sec,seq:tca)
     gene            2161..2325
                     /locus_tag="OO249_004147"
                     /pseudo
     CDS             2161..2325
                     /locus_tag="OO249_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280578.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
BASE COUNT          641 a          596 c          558 g          586 t
ORIGIN      
        1 agcataacgt tctgtgtttt cgattgtgac cttcacgtcc tgcaaaatat tgagcggata
       61 atgaggaccg ttattcagcg ctccctgaaa atgctcaatc cgcacaccga caataccttc
      121 ctgtggggag aaaaagcgca gcgtaaataa aggcgtatca agctgccagg tacgttcacg
      181 cacatcacgg ggggcagcat agaccaccat ttcattatcc tgctgttcaa cctcgaacac
      241 ctgaagcggg tgaatcaaat tgaggccagg ttgaatcaac cagtttccat cgctaatttt
      301 cattgccgtt ccttagttct gcacttcttg agaggtgaat tgttgctggc aacggcgttt
      361 accctgagcc agctgttcca taacggtttt cagattgtgc gtcgtgagtg agtagtagcg
      421 tttagcgata atcgcgctca gcaaataaca gatcgccgga acaatcgtga atagcgcaat
      481 aatgatgcta atcgtggcgc tgttctgcgc tttttctgcc gcatcatatc cgccataagc
      541 cagcatccag ccgataagag cgccgccgaa ggccaacccc agtttgagca caaacagcgt
      601 gccagcaaaa ctgatcccgg tcaggcgttt accattgcac cactcgccgt agtcgacggt
      661 atcggacatc attacccact ggataggtgt caccagttga tgcaacacac caatcacgaa
      721 gatgaagaca aacatagtga tgctggcctg catgggaaca aagaacatcg cgaggctaat
      781 cactgccagc agggcgttcg tccaccagaa gatagtgact ttacatttcc agtcggtcag
      841 aggttttgcc agtgcggaac caatcaggtt acccacgcaa taagtggtga gaaaagcgac
      901 aaacacttcc ggcgtgccca aaatccatgt gacgtaatac atcatcgccc caccgagtac
      961 gcacaccgcc aggatattga aaatggttag taaaccgaca atccgccact ggtcgttttg
     1021 ccagatatca cgtaaatctt cccgcataga cgttgttgta ggtggtgctt caacgcgttc
     1081 tttagtggtg aagaaacaaa atgccagcat catgaatgcc accacggaaa ggaccgcgat
     1141 accgccctgg aaaccgagtg gtttattatc accgccaatt aaattaacca gtggcatcat
     1201 cagaacagta gaaagcatgc ctcccgcggt cgccagcaca aaacgccagg attgcagcga
     1261 gatacgctga gtcgggtcat tggtgattac accacccaat gcgcagtaag ggatattgac
     1321 gacggtataa agtaaggtaa gtagggtgta agtaattgct gcatagatca ttttgccgtt
     1381 catactgaga tctggcgtgc tataggccag tacacagacg atcccgaatg gcagtgcgcc
     1441 aaacagtacc cacggacgaa atttacccca gcgagagcgc gttcgatcgg ccaacaaccc
     1501 catgcaagga tcggaaatcg catccagtgc acgagcgacc aaaaacatgg ttccgacaaa
     1561 accggcagga atgccaaaaa tatcggtata aaagaacatc atatataaca ttacgttatc
     1621 gaaaataatg tggctggcgg cgtctcccat gccataacca attttctctt taacggacaa
     1681 cacttcactc ttcatctgct tttccttgat gctatagggg tgctgagacc gataacgcat
     1741 tttgtaaacc atccctgtga gagcgaatat tccgatatct ggttattaat ttatgattct
     1801 tgttttatgt gatcgtggta gcgttaattc cgctcatatg tcattgtaaa atatgggttt
     1861 tatatgaact ataatgcttt cgtgataata cgctgcgtgt attaggcgga aaaaactgat
     1921 ctgggggatg tagaaactca aggaagtagc tataatgcgc cccgcctcca tgtagcaatc
     1981 gaggcgcgga agatcgtcgt ctccggtgag gcggctggac ttcaaatcca gttggggccg
     2041 ccagcggtcc cggacaggtt cgactcctgt gatcttccgc caaaatgcct cttctgacgt
     2101 tctccaaccc cttgtaatac tgtcaatcca gatattttct actgctcaat attcaatcgg
     2161 cttcaggaat acctggtcta aggttttgtg atggacgatg agcggctgaa gaatccgcct
     2221 gtcggttctt ctgtcctcga acgcatccgc gatatgcgcg ccagcatgtg ccgtgtttat
     2281 ttgcaggtga tttgccccct gctggctctc agccaactgt atcgccatta aggtgaagat
     2341 gttgttttac acgctccata acaccctttt ttatacgact a
//
LOCUS       DAJTOR010000298         2291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTOR010000298 DAJTOR010000000
VERSION     DAJTOR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.305"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO249_004148"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO249_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5638223.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO249_004149"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO249_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5638224.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO249_004150"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO249_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5638225.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          613 a          487 c          540 g          651 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agcaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaggg caacagatcg cgtacccagt
     1021 ttgccggcca gtcttggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattca ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatacgataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aaccacggca gcctggcggc
     2161 tgcaaatcgg ggtgtggcag aatatgagct ttcagagtaa ccatccagaa tgagttccca
     2221 tgtcacacaa cctggagcac cagaaagtcc acaccagaat ggtgaaagag gttctgaaag
     2281 ccgtcgccag a
//
LOCUS       DAJTOR010000299         2264 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTOR010000299 DAJTOR010000000
VERSION     DAJTOR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.306"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            376..519
                     /locus_tag="OO249_004151"
                     /pseudo
     CDS             376..519
                     /locus_tag="OO249_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            666..934
                     /locus_tag="OO249_004152"
                     /pseudo
     CDS             666..934
                     /locus_tag="OO249_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1327..>2264
                     /locus_tag="OO249_004153"
     CDS             1327..>2264
                     /locus_tag="OO249_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5638226.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLAMANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEHSLADAVTAWFPENKQSDVSQIWHAFEHEE"
BASE COUNT          754 a          484 c          386 g          640 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcaaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aacgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacgtaaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaat aaatttttta ccgaaatata acataaggaa
      661 gtaatatgct tacacaaact atattccctt gtcttcccaa aaaacaagaa aatatcatcc
      721 ctgaagtatc aaatactata ttagtatctt ctactgttac tacagatgga tgtactgttt
      781 ttaataaaca aaaacagcca tatacgaatt gcaaatacct gctaccaacc gaacgaaaaa
      841 gttgaaattc acagctacag aaatacaatg gttgacaaag ataaatgaag ccggaattga
      901 tgaaaaacaa agccaacggt attctgattt ctgaagttaa tttcagactc gtttaagctc
      961 cattttcaaa aaaaaatata tcagggtttg ttactaataa cttctgaaaa cagaaaaaaa
     1021 atagagcagt aattctattt cagagaggag taactccatc tatcctcgat acgtactgaa
     1081 cgttatcgtg atatattcat catgctttct tcaccattat tcgagtacag ggagagacgc
     1141 aatgacctta aatacctttt tatggctgaa ttaccattct caggaataac agcagactcc
     1201 ttcccataga cggcttaggg ggaaagcaat acccggagaa agggtacaaa gcaaactgtt
     1261 ttaaaattaa taagctatat tctatttcac tagaattctt atcgctgata agaaatttta
     1321 gttataatga ttaaatcaac caatatacag gcaatcggtt ctggtattat gcatcaaata
     1381 aacaatatat actcgttaac tccatttcct ttacctatgg aactgactcc atcttgtaat
     1441 gaattttatt taaaagcctg gagtgaatgg gaaaagaacg gtaccccagg cgagcaacgc
     1501 aatatcgcct tcaataggct gaaaatatgt ttacaaaatc aagaggcaga attaaattta
     1561 tctgagttag atttaaaaac attaccagat ttaccgcctc agataacaac actggaaata
     1621 agaaaaaacc tattaacaca tctccctgat ttaccaccaa tgcttaaggt aatacatgct
     1681 caatttaatc aactggaaag cttacctgcc ttacccgaga cgttagaaga gcttaatgcg
     1741 ggtgataaca agataaaaga attaccattt cttcctgaaa atctaactca tttacgggtt
     1801 cataataacc gattgcatat tctgccacta ttgccaccgg aactaaaatt actggtagtt
     1861 tctggaaaca gattagacag cattcccccc tttccagata agcttgaagg gctggctatg
     1921 gctaataatt ttatagaaca actaccggaa ttacctttta gtatgaacag ggctgtgcta
     1981 atgaataata atctgacaac acttccggaa agtgtcctga gattagctca gaatgccttc
     2041 gtaaatgttg caggtaatcc actgtctggc cataccatgc gtacactaca acaaataacc
     2101 accggaccag attattctgg tcctcgaata tttttctcta tgggaaattc tgccacaatt
     2161 tccgctccag aacactccct ggctgatgcc gtgacagcat ggttcccgga aaacaaacaa
     2221 tctgatgtat cacagatatg gcatgctttt gaacatgaag agca
//
LOCUS       DAJTOR010000300         2250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTOR010000300 DAJTOR010000000
VERSION     DAJTOR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.307"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            111..432
                     /locus_tag="OO249_004154"
                     /pseudo
     CDS             111..432
                     /locus_tag="OO249_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(466..672)
                     /locus_tag="OO249_004155"
     CDS             complement(466..672)
                     /locus_tag="OO249_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638227.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1170..1243)
                     /locus_tag="OO249_004156"
     tRNA            complement(1170..1243)
                     /locus_tag="OO249_004156"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1209..1211),aa:Gly,seq:ccc)
     gene            complement(1322..2077)
                     /locus_tag="OO249_004157"
     CDS             complement(1322..2077)
                     /locus_tag="OO249_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5638228.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKTGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
BASE COUNT          601 a          527 c          491 g          631 t
ORIGIN      
        1 tcatacacta aatcagtaag ttggcagcat taccaatttt gctaagttaa tgcattaatc
       61 taaaaacgct ttaaatcaat acataaccat tatctcttgg gattatttac atgcgcaaaa
      121 gtttaatgct gttacttgct tcaacattga ttatttctgg atgtgcaaca aaaaaacaac
      181 tcgttccaac tggtggaagc aaatcagacg gcacagttag gatgagttat tcttacggta
      241 tgtttgaaag tcctgttgta gatccacaac aaggcatgaa tgcagccaaa gcgcgttgtg
      301 ccgcttaggg gatattcagg agccgagcct tttggtggat tcacttctca atgttcacaa
      361 ccttcttcat ctggttgtat gcaaacactt gtgactatcg aatatcaatg cactggcgat
      421 attaagaagt aataattagg ggcttccagc cccttctata accattcatg gaacagggac
      481 aaccgaaatc actttaaacc ctgaaacagc cgttactcca ttagatggag aattaaatct
      541 cacagtatct tttcttactg catcatgaat ttcatctttt gttggttcat gctctgaatg
      601 gaataaaacc tcttccattc taatttgatc accgtctcta tgctcataag taatcttgta
      661 gctaacagtc atatcaacct catgcagggg aattgtgaga ttattataga caatagaaac
      721 cataaagccc cgttggtgat acagcaacaa gcgggagcgt agcgacttca tcagattgtc
      781 agaaagatca caccttatca ggataaccgt gtacccgttg ggagcgtggc tgaaacgctg
      841 atagagcttg catatggttt gcaaggacca atatcaccat aaacctgttt acgcagccac
      901 aaggccgcac atcgggctta aatgcgtgtg ttcagtctgt cgatgacatt acaatgccag
      961 gatgggcttt atcttcacgt tgtgctatcg cggtgtgaga ggtgcaactg ccgccaacgt
     1021 taggttatac tgtcatctca ccgtgtaact taacgtgaca gttactaagg gcggatcaca
     1081 atggtagaac tcctaagcaa attaaatagg taactgttta agttgctgac agagaagact
     1141 tttttagtct tctccacctg gggagattct ggagcgggcg aagggaatcg aaccctcgta
     1201 tagagcttgg gaagctctcg ttctaccatt gaactacgcc cgcttcgaga tgcgtaaggc
     1261 attataaacc ttacgctctc cttagcaagt gccgcgctgc tgactgctga ttaattcgcc
     1321 atcagcattt tggcttgctg ccctgcggcg gcaagtaacg tagcggatca attgccgttg
     1381 cacggtaacg aatctggaaa tgcaggcgaa cagatgccgc atccgtgctc cccatagtgg
     1441 cgattttttg cccagtcttc acgctttgcc cattatttac cagcatcgtg tcattatggg
     1501 cgtaagccgt aatgtaatct tcactgtgtt taatcatgat gagattaccg tagccacgca
     1561 gctggttgcc cacatacacc acctttcctg cacccgcggc gtaaataggt gtaccccgtg
     1621 gagctgagat atcaatccct ttattgccgc catctgctgt cgaatacggc atgataactt
     1681 tccctgtcgt tggccataac caacaacgtt gccctactgg cggccaggaa gatttcggta
     1741 ccgctgatga cggtgtaacc gatgcggttt tggtcgttga tttggcggtt gatttacgtg
     1801 tactactgct acttttcgcc ccacccagtt ttagtttctg accaacttca atggtgtaag
     1861 ggggggaaat gccgttcagt cgcgccagtt cttttacgct ggttcccgtg gtgcgcgaaa
     1921 tacgatatag cgtatccccc cgtttcacgg tgtaaacgga gccggaatat gttcctgtat
     1981 cggatgattt gctacccgaa cagcccgcca aaagcagtcc aaccgataac aacatcacga
     2041 tacccagaga ttttttattc aggcgtcccg cactcaaaat ctttcctcgc ttaaaaaaca
     2101 agacgcctta tcatagcagc cagcctcttg atacccaatc tcttgcttct ggaaaaacgt
     2161 ggtaatagag aaagaggtta atttcatttg gcaaagggaa gtatcatcag caaaatattc
     2221 gctgtagagg tatcgaactt cctgactttt
//
LOCUS       DAJTOR010000301         2223 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTOR010000301 DAJTOR010000000
VERSION     DAJTOR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.308"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..125
                     /locus_tag="OO249_004158"
     CDS             <1..125
                     /locus_tag="OO249_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5638229.1"
                     /translation="HDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
     gene            complement(145..396)
                     /locus_tag="OO249_004159"
     CDS             complement(145..396)
                     /locus_tag="OO249_004159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638230.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            319..1009
                     /locus_tag="OO249_004160"
                     /pseudo
     CDS             319..1009
                     /locus_tag="OO249_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1026..1205)
                     /locus_tag="OO249_004161"
                     /pseudo
     CDS             complement(1026..1205)
                     /locus_tag="OO249_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1535..>2223
                     /gene="ipaH7.8"
                     /locus_tag="OO249_004162"
     CDS             1535..>2223
                     /gene="ipaH7.8"
                     /locus_tag="OO249_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5638231.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
BASE COUNT          663 a          500 c          413 g          647 t
ORIGIN      
        1 ttcacgacac aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag
       61 ttcgccattt tatggaaacc gtcagcccat tccccggagg caaacatggg ctggcaaaag
      121 tgtagcggta ttaagcgcag ctatttagat tattgccgca ttttctccac cacgatcaga
      181 gccgaaaaaa gataattttt ttccaactgc cacccggcgt aatgcgtttt caccaatgtt
      241 accccaccta ttgcggcaaa aggtaatgtc ttcaatcgat agaagtttct ggtaatctac
      301 tggtatttga ggttttccat gactaccgtt actgttcatt gtccgcgctg taattccgat
      361 gaagtttatc gacatggccg tagttgttcc cggcatgagc gtttccgttg tcgctcatgt
      421 aaacgtgttt tccagctcac ttactcttat gaagccagaa aactcggcgt taaggagcag
      481 atcgttgaaa tggcacataa cggcgcaggg ggctgttata ccgccagaac actgaaaatc
      541 ggcatcaata ccgtcatacg gacattaaaa agctctcgcc ccggcggata acatctgcgc
      601 cagtcgctca cgctgatgtt gcccttatct gtgaacttga tgagcagtgg cgcttcgttg
      661 gcagcaaggc gcaacaacac tggccctggt atgcgtacaa cactaaaact ggcggtgtgt
      721 tggcttacac ttgcgggcca cggaatgatg agacctgtcg ggaactgtta gccctgttga
      781 ctccgttctg tatcggcatg gtgaccagcg atgactgggg cagctacgcc agagaagtgc
      841 cggaggagaa acacctgacc ggcaaaatct tcacgcaacg tatgaacgta acaacctgac
      901 cctccgcccc cgcatcaagc ggctggcacg taaaacaatc tgcttctcgc gttccgtgga
      961 aatccacgaa aaagtcatcg gttccttcat tgaaaagcac atattctaat gattggaagt
     1021 actacccggc aaaactcatt cagtgcattc cagtgattca ggatatagtt gaacgccttt
     1081 accatttcag catggtttga caaagttttc ctctggagct ttatccagtc atacaaggac
     1141 ttcattcgcg gcacacattt ctcctgcctt accgcaagcc gtttttctgc cggcctccct
     1201 catattaaat gtgtatcgtt tttttacagt aattttaatt tatcattcta taataggaat
     1261 aaatgagctt ggctaaaata tgatgtaaaa caaatatact tactcggatt acatttcctt
     1321 gctcactgga atgttgaatt cagtgttatt attgactgca gtcacaataa aaaaaagaat
     1381 tccatagaaa acctcaacaa ataaattaca actaacttct gttatgtgta aaatggaaac
     1441 tattaaaact taatatcgaa aatggtaagt gaaatttgta taaatataca attttaaata
     1501 tttattctca caaatataag gttgccctag cattatgttc tctgtaaata atacacactc
     1561 atcagtttct tgctccccct ctattaactc aaactcaacc agtaatgaac attatctgag
     1621 aatcctgact gaatgggaaa agaactcttc tcccggggaa gagcgaggca ttgcttttaa
     1681 cagactctcc cagtgctttc agaatcaaga agcagtatta aatttatcag acctaaattt
     1741 gacgtctctt cccgaattac caaagcatat ttctgctttg attgtagaaa ataataaatt
     1801 aacatcattg ccaaagctgc ctgcatttct caaagaactt aatgctgata ataacaggct
     1861 ttctgtgata ccagaacttc ctgagtcatt aacaacttta agtgttcgtt ctaatcaact
     1921 ggaaaacctt cctgttttgc caaaccattt aacatcatta tttgttgaaa ataacaggct
     1981 atataattta ccggctcttc ccgaaaaatt gaaattttta catgtttatt ataacaggct
     2041 gacaacatta cccgacttac cggataaact ggaaattctc tgtgctcagc gcaataatct
     2101 ggttactttt cctcaatttt ctgatagaaa caatatcaga caaaaggaat attattttca
     2161 ttttaatcag ataaccactc ttccggagag tttttcacaa ttagattcaa gttacaggat
     2221 taa
//
LOCUS       DAJTOR010000302         2214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTOR010000302 DAJTOR010000000
VERSION     DAJTOR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.309"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            11..1129
                     /gene="potA"
                     /locus_tag="OO249_004163"
     CDS             11..1129
                     /gene="potA"
                     /locus_tag="OO249_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5638232.1"
                     /translation="MNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGEFLTLLG
                     PSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFPHMTVFE
                     NVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIARAVVNKP
                     RLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRIVVMREG
                     RIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGRECNIYVNF
                     AVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELENGKMVM
                     VSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            1113..1976
                     /gene="potB"
                     /locus_tag="OO249_004164"
     CDS             1113..1976
                     /gene="potB"
                     /locus_tag="OO249_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5638233.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
BASE COUNT          518 a          540 c          563 g          593 t
ORIGIN      
        1 gagtaaaaaa ttgaataaac aaccgagttc gctttcaccg ctggtgcaat tggcgggaat
       61 tcgcaaatgc tttgatggta aagaggtcat tccccagctg gatctgacta tcaacaatgg
      121 cgagttcctc acgctgcttg gcccttctgg ctgcggtaaa acaaccgttc ttcgcctgat
      181 tgcaggtctg gaaactgttg attccggacg catcatgctg gataacgagg acatcaccca
      241 cgttccggcg gaaaaccgct atgtgaacac tgttttccaa agctacgcac ttttccccca
      301 catgaccgtg ttcgaaaatg tggcctttgg gttgcgcatg caaaaaaccc ccgctgctga
      361 aattacgccc cgcgtgatgg aagccctgcg gatggtgcaa ctggaaacct tcgctcaacg
      421 taaaccgcat cagctctctg gtggtcaaca gcaacgcgtc gccattgctc gcgcggtggt
      481 taacaagcct cgtctgttgt tgctggatga gtcgctctca gcgctggatt acaaactgcg
      541 taagcaaatg cagaacgagc tgaaagcgtt acagcgtaag cttggcatta cattcgtctt
      601 cgtgactcac gaccaggaag aagcactcac catgtcagac aggattgtgg tgatgcgcga
      661 gggtcgcatt gagcaagacg gcacgccgcg tgaaatctac gaagagccga aaaacctgtt
      721 tgttgccggc ttcattggcg aaattaatat gtttaacgcc acggtgatcg agcgactcga
      781 cgagcagcgc gtacgcgcca acgttgaagg ccgcgaatgt aatatctacg ttaacttcgc
      841 cgttgaaccg gggcaaaaac tgcatgttct gctgcgcccg gaagacttac gtgttgaaga
      901 gattaacgac gacaaccacg ctgaagggct gattggttac gttcgtgagc gtaactacaa
      961 aggcatgacg ctggagtcgg ttgttgaact ggaaaatggc aagatggtga tggtcagcga
     1021 attcttcaat gaagacgatc ctgactttga ccactctctc gaccaaaaaa tggccattaa
     1081 ttgggtagaa agctgggagg tcgtactggc tgatgaagaa cacaagtaag ttccagaatg
     1141 tagtgattgt cactattgtc ggttggcttg tgttgtttgt ctttctgccc aacctgatga
     1201 tcattggcac cagctttttg acccgcgacg acgccagttt cgtcaaaatg gtctttacgc
     1261 tggataacta cacgcgtctg ctcgatccgc tctattttga agtgctattg cactcgctga
     1321 atatggcgct gatcgccacc ctcgcctgcc tggtgctggg ctatccattt gcctggtttc
     1381 tggcgaagct gccacacaag gtgcgtccgc tgctgctgtt tctgctgatt gttccgttct
     1441 ggaccaactc attgattcgt atctacgggc tgaaaatttt cctcagcacc aaaggctatc
     1501 tcaacgagtt tttgctctgg ctgggcgtta tcgacacacc aatccgtatc atgttcacgc
     1561 ccagtgcggt gattatcggt ctggtttaca ttctgctgcc gtttatggtg atgccgctgt
     1621 actccagtat cgaaaaactg gataagccgt tgcttgaggc ggcgcgcgat ctcggtgcca
     1681 gcaagttaca gacttttatc cgcatcatta ttccactgac gatgccgggg attattgccg
     1741 gatgtctgct ggtgatgctg ccagcgatgg gcctgttcta tgtgtccgac ctgatgggcg
     1801 gtgcgaaaaa cctgctgatc ggtaacgtca tcaaggtaca gttcctcaat attcgtgact
     1861 ggccgtttgg tgcagccacc agcattacgc tgactatcgt aatgggcctg atgttgctgg
     1921 tttactggcg cgcttctcgt ttgctgaata agaaggtgag cgaattagat gattaatcat
     1981 ttatattcaa tgcattaagt ttgtttttga gtggttttgc cgtttaaggt aacggcgtta
     2041 tagatcctat caaaatcaat caattccatt ctggttttgg agaaagattt ttcgagagct
     2101 tcggtcttta actaaaactg attgcgtata accccaatac ccctccccga ctatctgaat
     2161 ggtcggggag gcacttcacg cacaaaatat catccctgcg ccgttgatta ctct
//
LOCUS       DAJTOR010000303         2189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTOR010000303 DAJTOR010000000
VERSION     DAJTOR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.310"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            718..>2189
                     /locus_tag="OO249_004165"
     CDS             718..>2189
                     /locus_tag="OO249_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5638234.1"
                     /translation="MAFKHYDVVRAASPSDLAKQLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLG"
BASE COUNT          532 a          557 c          600 g          500 t
ORIGIN      
        1 ctgtgccgga tacatcacag tttctggaac cttaggtact acagtatcag tcagggtgct
       61 atattttcag atgtgatgaa agcctgtcag caggcagggc gtatcggaaa tgacccagta
      121 gagaaaacgt tgactcagat accggtgctg agttaccggg aaaccggcat cacatgaccg
      181 ctatccttcc aggcccatcc gctccggtgg gcctttttac tgcagaaaac aggttccccg
      241 ttaaatgcta tgttgctcac aattcagtaa gttgacagtt gcctgtcaga ctgggcattt
      301 gttaaaaaaa tttcgcatgg tgaatccccc tgagcggagg ggcgactggt gacggtataa
      361 tctctgatta tcaaaacgag aatgacgcgg gtttagtggc accgggctga actcaccggg
      421 aggcacccgg caccatgtgc atgatgatac agatacgcgg ctttagcccc tctccggagg
      481 ggctttctta tggacaaaaa aagcccgcgc tgggagacgc gggcggcaag gaataaacaa
      541 taaaacgtga agtaatattt cagctggcga ataatacccc atagtaatca ctctgcgcaa
      601 ctgcgcggtc tttttcgaat tgcgggctgt agtctccctt ctgccattgt cctgtaactt
      661 ccggacttca gcctgctcct tatctgactc acaacattat cccgcccggg aggattcatg
      721 gcatttaaac actatgacgt ggtcagggcg gcatcgccgt cagaccttgc gaaacaactg
      781 acacaaaaac tgaaggaggg ctggcagccg tttggtagtc cggtggccat aaccccttat
      841 accctgatgc aggcgattgc agcagaaggt gatgtggtcg tcagtggtgc aactgagccg
      901 gagtggtact acgtcatcgt actggccggg caatccaatg ccatggctta cggtgaaggg
      961 cttccgcttc cggattcata cgatgcgccc catccgcgca ttaagcaact ggcccgtcgt
     1021 aacacagtga ctcccggtgg tgaagtatgc gtatttaacg acatcattcc tgctgaccat
     1081 tgtctgcatg atgttcagga tatgagtacg attaaccatc cccgggctga cctgagcaaa
     1141 gggcagtacg gctgtgtcgg acagggctta catattgcca aaaaactgct tccgtatatc
     1201 cctaataatg cggggatcct gctggtacca tgctgtcgtg gtggttcggc attcacccag
     1261 ggcacggagg ggacattcag cgagtccacg ggagccagtc aggattcggc tcgctgggga
     1321 gtgggtaagc cgttatatca ggatctgctt ttccgcacga aggcagcatt gcagaaaaac
     1381 ccgaaaaacg ttttgctggc gatatgctgg atgcaggggg aattcgatat gacgaatgcc
     1441 agttacgccc agcagccagc agcatttctt gcaatggtac agcagttccg tgctgacctt
     1501 gccgggctgg cagcgcagtg tcacggtgga agtccggcat cagtcccctg gatttgtggc
     1561 gacacgacat acgcgtggaa acaagaacac ggtacgcaat atgaagtggt atatggtgca
     1621 tataaaggta aagaatccca gcagatttat tttgttccct ttatgaccga tggtagcgga
     1681 gttaatacac cgacaaacaa cccgtcagaa gatcctgata ttgccgggtc tggttattac
     1741 ggttcggcat cccgaacgaa caaaaactgg gtatcatcaa atcgcccgac gcatttcagc
     1801 tcatgggcgc gtcgtggcat tattcccgat cgtatggcaa ctgctattct gaacgtagcc
     1861 ggtcgcacct tagccttcat tagtggtaag gcaccggaaa tcaaaccctc gcccggcggc
     1921 gacacgccat cggggccgtc tgaagatgca tccgtacgca caatctccct gttgccgaca
     1981 gccggagatg ctgctgcgca gggctggagc attaagaatg gcggaattca gttgtcagat
     2041 ggtgtattta agatcaccaa gcagagcaat aaagcctggt ccctgacgcg cccggtggat
     2101 gacgcagtct ccctgctgac acggggtggc agactgagct gtaagtttcg actgtcaggc
     2161 gcactgacca acaaccagtt cggtctggg
//
LOCUS       DAJTOR010000304         2161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTOR010000304 DAJTOR010000000
VERSION     DAJTOR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.311"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            91..593
                     /locus_tag="OO249_004166"
                     /pseudo
     CDS             91..593
                     /locus_tag="OO249_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(639..1055)
                     /gene="rraB"
                     /locus_tag="OO249_004167"
     CDS             complement(639..1055)
                     /gene="rraB"
                     /locus_tag="OO249_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5638235.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1217..>2161
                     /gene="argF"
                     /locus_tag="OO249_004168"
     CDS             1217..>2161
                     /gene="argF"
                     /locus_tag="OO249_004168"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5638236.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFD"
BASE COUNT          544 a          509 c          547 g          561 t
ORIGIN      
        1 aactttattg ctttataaga tgcttgcgca cgtaagaaga gagtggtatt aaacaaatgt
       61 ataattactt tatttttatt gggttaaact atgaataaca ttgcgccgca atcacctgta
      121 atgcgtcgcc tgacgctaca ggataatcct gctattgccc gcgtcattcg tcaggtatcc
      181 gccgaatacg gtcttaccgc tgataaaggc tacaccgtcg ccgatccgaa tcttgacgaa
      241 ctgtatcaag tgtatagtca gccgggtcat gcatattggg tggttgagta cgagggtgaa
      301 gtggtcggcg gcggtgggat tgcgccatta gcaggtagtg aatcggacat ttgcgaactg
      361 caaaagatgt attttcttcc ggctattcgc ggtaaagggc tggcgaaaaa actggcctta
      421 atggcgatgg agcaggcacg agagatgggc ttcaaacgct gctatctgga aacgaccgct
      481 ttttaaagga agccattgcg ctttatgagc atttgggctt tgagcatatc gactatgcac
      541 ttggctgcac gggccatgtt gattgtgaag tgcggatgtt gcgcgaactc taaaaaaatg
      601 cccgctagtg tgtgctatcg ggcattttaa tcaattaatt agtggcgaac cccgtcatcg
      661 tcttcatcga caaaatcttc atcgtcgccg tcttcgccat taggatcttc aaaataagtg
      721 ccccaaccgt cgtactcaac gtcaaatttc tccgccaatg tcatcagttg ttcaacctgg
      781 gcatcgatca gatcggcatt caacgcgcac tcgctgagga tgtcgcagca aatcacgata
      841 tcaccatctt caacttccag ctcttctgga tcggtcactt cgtaaccgag tttaaacgct
      901 tcaactgctg ctttttccag ggtttccaga tcgtctgcgg aaagatggtg ttcgatggtg
      961 tacagcgcgt ccggatcgct gccatcttcc agtaattctt caataatcaa acgagtttct
     1021 tcacgctgtt cttccagttg ttccgggttt gccatggctc attcctctta atgtcctgcc
     1081 gatactctta ttgtcacata ccactgacat tgcctccacc tttgtgacaa agatttatgc
     1141 tttagacttg caaatgaata atcatccata taaattgaat tttaattcat tgaggcgtta
     1201 gccacaggag ggatctatgt ccgggtttta tcataagcat ttcctgaaat tactcgattt
     1261 cacgccagct gaactcaaca gcctgctgca gttagccgcg aagctgaaag ccgataagaa
     1321 aagcggcaaa gaagaagcca aactcactgg taaaaacatc gcgctcatct tcgaaaaaga
     1381 ctcgacccgt acccgatgct ctttcgaagt tgccgcatat gaccagggtg ctcgcgttac
     1441 ttatctcggc ccaagcggca gtcagattgg tcataaagag tcgattaaag acactgcccg
     1501 cgtgctgggt cgcatgtatg acggtattca gtatcgcggc tatggtcagg agattgccga
     1561 aacactggcg gaatacgctg gcgtgccggt atggaacggc ctgaccaatg agtttcaccc
     1621 cacgcagttg ctggcggatc tcctcaccat gcaggagcat ttacccggta aagcattcaa
     1681 cgaaatgacg ctggtctatg caggtgacgc acgtaacaac atgggcaatt cgatgctcga
     1741 agctgcggcg cttaccggtc tggatttgcg tctggtcgcg ccacaggcat gctggccgga
     1801 agctgcgctg gttacggaat gccgcgcctt ggcacagcaa aatggcggga atattacgct
     1861 gacggaagat gtagccaaag gagttgaagg tgctgacttt atctataccg atgtatgggt
     1921 gtcgatgggg gaagcaaaag agaaatgggc agaacggatt gcattactgc gtgattatca
     1981 ggtgaacagc aagatgatgc agttgaccgg taacccggag gtcaaattcc tccactgcct
     2041 gcccgcgttt catgacgacc agacgacgct tggtaagaaa atggcggaag agtttggcct
     2101 acatggcgga atggaagtga ctgatgaggt cttcgaatct gccgccagca ttgtttttga
     2161 t
//
LOCUS       DAJTOR010000305         2095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTOR010000305 DAJTOR010000000
VERSION     DAJTOR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.312"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..116
                     /locus_tag="OO249_004169"
     CDS             <1..116
                     /locus_tag="OO249_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5638237.1"
                     /translation="VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            113..463
                     /gene="tnpB"
                     /locus_tag="OO249_004170"
     CDS             113..463
                     /gene="tnpB"
                     /locus_tag="OO249_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5638238.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDMIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            494..2086
                     /locus_tag="OO249_004171"
     CDS             494..2086
                     /locus_tag="OO249_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5638239.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARQGVELSRALLSNWVDACCQLMTPLNDALYRYVMNSRKVHTDDTPVKVLAPGRKKAK
                     TGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLSPFRGILQADAFNGYDRLF
                     SAEREGGALTEAGCWAHARRKVHDVYISTKSATAEEALKLIGELYAIEHEIRGLPVSE
                     RLAVRQMQSKPLLTSLYKLMQEKEHTLSKKCRLRDAFRYIRKHWVALCNFSDDGLAEA
                     DNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRYILSV
                     LPEWPSNRVDELLPWNVALTNK"
BASE COUNT          527 a          529 c          602 g          437 t
ORIGIN      
        1 cagttagtgc cagctcctgc aaggtggagt tccgtcacgg taacatgacg ctggaaaatc
       61 cttcaccaga gctgctcaca gtgttgatcc gtgaactgac cgggagggga cgatgatctc
      121 actcccgtca ggcacccgca tctggctcgt tgctgggata accgatatgc gtaagtcttt
      181 caacgggctg ggtgaacagg tacagcatgt gctgaatgat aatcccttct ccggtcacct
      241 gttcatcttc cgtggccgac ggggtgacat gattaaaatc ctgtgggctg atgctgatgg
      301 tctgtgcctg ttcaccagac gcctggagga aggccagttt atctggcctg ctgtgcgtga
      361 cggcaaggta tccattaccc gctcgcaact ggcaatgctc ctcgataagc tggactggcg
      421 tcagccaaaa acatcccgcc ttaatgcact gacaatgttg taaaaatgtc atggccggat
      481 tataaaaacg gccatgaatc aaaaatacct cattcgcatt gcagaactgg aatgccagct
      541 ccgtcagaaa gaccagcaac tgagtctggt tgaagagacg gaggccttcc tgcgctctgc
      601 actggcccgc gccgaagaaa agatcgaaga agatgaacgg gaaatagaac atctgcgggc
      661 tcagatagaa aaactgcgcc ggatgctgtt cggtacccgt tctgaaaaac tgcgtcgtga
      721 agttgaacag gctgaggccc tgctgaaaca acgcgaacag gacagtgatc gttacagtgg
      781 gcgggaagac gatccgcagg ttccccgcca gttgcgacag tctcgtcatc gtcgcccgtt
      841 accggagcat ctgccccgcg aaataaatcg cctggagcca gaagaaagct gttgcccgga
      901 gtgtggcggt gagctggatt atctggggga agtcagcgca gaacaactgg aactggtgag
      961 cagcgctctg aaagtgatcc gcacagaacg ggtaaaaaaa gcctgtacaa aatgtgactg
     1021 catcgttgaa gcaccggcac catcccgtcc gatagagcgt ggtatcgcgg gcccggggtt
     1081 acttgcccgc gtgttaacgg gaaaatactg cgaacacctg ccactgtatc gtcagagtga
     1141 aatttttgcc cgtcagggtg tcgaactgag ccgtgcatta ctctccaact gggttgacgc
     1201 gtgctgccag ttaatgacgc cgctgaatga tgctctgtac cgttatgtaa tgaacagccg
     1261 caaagttcac actgatgaca caccagtaaa agtgctggca ccgggcagga agaaggcgaa
     1321 aacaggatat atctggacgt atgtccggga tgacaggaat gccggttcgc cagagcctcc
     1381 ggcggtctgg ttcgcctact caccggacca tcagggtaaa catccggagc agcaccttag
     1441 tcccttccgg ggtatcctgc aggcagatgc gtttaatggt tacgatcggc tgttcagtgc
     1501 cgaacgagaa ggcggcgcgt tgacggaagc aggatgctgg gctcatgcgc ggcgcaaagt
     1561 ccacgatgta tatatcagta ccaaaagcgc gacagcggaa gaagccctga aactaatcgg
     1621 tgagctgtac gccatcgagc acgaaatacg cgggttgccg gtgtctgaac gcctggcggt
     1681 caggcaaatg cagagtaaac cgctactgac ttccctgtat aagctgatgc aggagaaaga
     1741 acacacgtta tcgaaaaaat gccgtctgag agatgcgttc cggtatatca ggaagcactg
     1801 ggttgcgttg tgcaacttca gtgatgatgg tctggctgag gcggataata atgccgcgga
     1861 aagagcgctt cgtgcagtct gtctcggaaa gaaaaacttt atgttcttcg gcagcgatca
     1921 cggtggagag cgtggtgcgc tactgtacgg gctgatcggc acctgccgac tgaacggtat
     1981 cgatccggaa gcgtatctgc gctatatcct gagcgtactg ccggaatggc cttccaaccg
     2041 tgttgacgaa ctcctgccat ggaacgtagc actcaccaat aaataagcgt caata
//
LOCUS       DAJTOR010000306         2030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTOR010000306 DAJTOR010000000
VERSION     DAJTOR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.313"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(48..384)
                     /locus_tag="OO249_004172"
                     /pseudo
     CDS             complement(48..384)
                     /locus_tag="OO249_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(917..1243)
                     /locus_tag="OO249_004173"
     CDS             complement(917..1243)
                     /locus_tag="OO249_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638240.1"
                     /translation="MFKYAEMLMAEEDNKVCELIFKQQDNLILKINDRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLILCALGVNSWRGKSKYLDYYLLRDEMPVRLVSIIFPTLL
                     ALFLLA"
BASE COUNT          667 a          382 c          355 g          626 t
ORIGIN      
        1 ataaatatga aaagatggtt taaatacccc gttacttttg acttacacta tacggtatcg
       61 catcgtttaa tattcgcacc ggccagattt ttatttctat tagttgtcac aatactgaat
      121 gcgtacgacc acagtattct ggctcctgtg tggtcatgct ttaattctgc gttccggaca
      181 gataagcagt tgcttgcagg aatccttctt gtgttaatgt cagttcccct ttttaccagt
      241 gctgatttcc acattccgtc caacagagct tatagccttt ccctggatta tagcattgtc
      301 cggctgaagt tctttttgaa taataataga agcactgctg gcagatccag tccgtttttc
      361 ataacccact gtactgataa ccataatcta atcagtagaa attgagtcga aaataagcac
      421 tactccatac aggataatta gaggtcagtt tgattattca caattcatta tcagcatttt
      481 ctgtttctga cgaaatcaac atgaaaagaa ccatatatga taattattat aataacggct
      541 ttaattggaa tacatatatt acaacgtatt atatataatt ggtattctgg gaactatatt
      601 ctcaaaatac agtagaaacg aggtatgttt ctggtggaaa ggacagtggg attaaaaagt
      661 aagaattgat aaaaaaacgc cagcaacaca tagtgccggc ggagggaata ccccatggag
      721 aaaatgtgat gcctagagca ccatgaatat ataattaaaa atagttagag ttgtcaatac
      781 tttcacaaaa ataattttcg tagtttcgta gtatagaaag atatttttat gcatgaccta
      841 cctgaatttg ctccgggtag aggttataaa taaaaattga atcacgacaa acacaatatt
      901 catagtatgg cgatgcctac gccagcaaga atagcgccaa taatgttggg aatataatag
      961 acaccagacg cacaggcatc tcatctctta acaaataata atcaaggtat ttacttttgc
     1021 ctcgccagct gtttacgcct aaagcacaca agattaacca ttcaataaca aatggcaata
     1081 tccatagcca tacgacttta ccttgtaatg ttcggtattt ctttataatt attctgggaa
     1141 atctatcatt tatttttaaa atcaaattat cttgttgttt aaaaataagt tcacatactt
     1201 tattatcttc ttccgccatc aacatctctg catacttaaa catttcagaa cgttccttta
     1261 gcacagaaga gtaattatat gtccagttcc caggcaataa tgcttatgga tatttaacta
     1321 ataatttaga gaatattttg caataatatt tggctgtatt cagaaatacc tgaaaaatca
     1381 tactatacag ccccagggaa tggataaaga ttctaacaaa gcatttcaac aatatatgct
     1441 tgttaaaaat ccatatcgaa atgccgttgc agcattaata tatgcctcat tcataaaatg
     1501 taaaagagca agctcgtacc agtagggggg aaattcattg acatgtcctg tcaatacgta
     1561 cagagccctg tcatgcttgc cagcacgtaa caattccgtc cccagtaacc agcgtgtccc
     1621 ctgattattc tcagggttat atgttagtat tttatcaatg agtataacgg catcctgatg
     1681 acgctgtaaa tgaacattcg ccagaattgc agcataaaga gcgcaaatgc atatgccaga
     1741 tgagcgtgaa tatcaatgat gtcaggagca tgtcaggcaa gacgcctgag tttgttgacg
     1801 taatttgttt cgtttatttc atcactatcg tatgcgtcaa gagttttttc aaactcatcc
     1861 cgaaatgggc caagcctggt ttcaggaaat aagaaatacc cctagttatt gttcacttaa
     1921 aagtataatt tgtaattcaa atcctgagcc tgtaaaacgg ggagtaacat attctattct
     1981 gaagagaata aaagtcgtga tgcgatgtat caagcccgga ttgtaatccc
//
LOCUS       DAJTOR010000307         2017 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTOR010000307 DAJTOR010000000
VERSION     DAJTOR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.314"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..1233)
                     /locus_tag="OO249_004174"
     CDS             complement(<1..1233)
                     /locus_tag="OO249_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5638241.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSS"
     gene            1410..>2017
                     /gene="kdgR"
                     /locus_tag="OO249_004175"
     CDS             1410..>2017
                     /gene="kdgR"
                     /locus_tag="OO249_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5638242.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQ"
BASE COUNT          600 a          469 c          540 g          408 t
ORIGIN      
        1 gctactctga cccagtagac gagccgttcc taacatgcca ctggctccac cgctacgttc
       61 gcgaggagcg gaggtaataa tggtgtggtt attgggtgac tggaacaagc caaatccagc
      121 gccacataag atcatcggcc agataatatt gatatccgca ggtgacgcgg gcagcagaac
      181 cagggaaaaa agccccgcag ccatgatgaa caaccctaga gcacccagca atcctgcatg
      241 tacgcgttca atcaaatagc ctgccaacgg agccatcacc atcgttgcta acggccacgg
      301 tgtcagaagt aaacctgttt cgacttcact acgcccgagc acggtttgca ggtaaaaggg
      361 cagggaaacc attgccagca tttgtgcgca gaaagagcaa acagatgtgc aaatagaaag
      421 tgaaaacagc gggatacgca gtaaatccac cggtagcagc ggtacgggaa gagaaagctg
      481 gcggcgaata aagaaaatac caacaacaac cattaccacc agttccgcag caattaacgt
      541 cagcgattgc ccctgagcga aaccactcaa cgcagtgata agcaggccga aggttaacgc
      601 gttcatcacg gcgctgggca ggtcgaaacg gggtttactg gcgcgagaac cattgggtgg
      661 cagaaaacgc atcgccagaa gcagggcgat aatacctaac ggtacgttga ttaaaaataa
      721 ccatttccag gatgcgatgg agaggattgc tgcagcaatt gtcggcccgg cagcagaaga
      781 gacggcaaca ataaacgagt ttatgcccat ccctctcccc agaaaacgtt gtggatagat
      841 caggcggata agtgcggtat taacgctcat caacgctgca ccgccgaaac cttgtatgac
      901 acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg cagaacagtg aagacaacag
      961 aaaaacgacc agaccgcatt tataaatacg tcgatagcca aacatatcgc ccagaaacga
     1021 aaacgagagc agggagatga caatggcgat ttgataggcg ttcactaccc agatggaact
     1081 ggctggcgtg gcatgaaggt ccgtggcgat tgttggcagg gcgacgttgg cgattgcgcc
     1141 gtcaaggaca gccatcgaaa taccaatcac aatggttaat atcgcaccgt atcgctgggg
     1201 caatggcagg ccgtcggcct gaacttttgg cataggaatt ttatatcttt ggtgaataat
     1261 caacaggggt atgattctag cactgatatt cacggtggat gtcgcagaat tgtaactaac
     1321 cggcaaaaga tggattgcga cccccctggt gcgaaattat aataaaaaac agttctgatt
     1381 tttataaaac actcgcaatg aggtgataaa tggctaacgc agatctggat aaacagcctg
     1441 attctgtatc ttccgtgcta aaagtttttg gcattttgca ggcgctgggt gaagagcgcg
     1501 aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc aaaaagcacc gtttatcgct
     1561 ttttacagac catgaaaacc ttaggttatg tggcgcagga aggggagtcg gagaaatatt
     1621 ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt acaaaacgtc gatttaattc
     1681 gtagcgcaga tatccagatg cgtgagatct cccgcctgac caaagaaact atccacctcg
     1741 gcgcactgga cgaagacagt attgtttaca ttcacaaaat tgactctatg tacaatttgc
     1801 gcatgtattc acggattggg cgtcgtaatc cgctgtacag caccgcgatt ggtaaggtac
     1861 tgctggcatg gcgcgatcgc gatgaagtga agcaaattct tgagggcgtg gagtataaac
     1921 gcagtaccga gcggaccatc accagtacag aagcgttatt acccgttctg gaccaggtgc
     1981 gcgagcaggg gtatggcgaa gataacgaag agcagga
//
LOCUS       DAJTOR010000308         1953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTOR010000308 DAJTOR010000000
VERSION     DAJTOR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.315"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OO249_004176"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OO249_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5638243.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTNV"
     gene            complement(1183..>1953)
                     /gene="ipaH1.4"
                     /locus_tag="OO249_004177"
     CDS             complement(1183..>1953)
                     /gene="ipaH1.4"
                     /locus_tag="OO249_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HCR5638244.1"
                     /translation="LDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADA
                     TESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVR
                     TLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENE
                     FKDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYSQRVADRLKASGLSGDTDAE
                     REAGAQVMRETEQQIYRQLTDEVLALRLSENGSRLHHS"
BASE COUNT          504 a          436 c          451 g          562 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgtcaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagcctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtcg
     1201 tgagccgttt tcagacaatc gcagggccag tacctcgtca gtcaactgac ggtaaatctg
     1261 ctgttcagtc tcacgcatca cctgtgcacc ggcttccctc tccgcatccg tatcaccgct
     1321 cagacctgat gctttcagcc ggtcagccac cctctgagag tactcattct ccagcatctc
     1381 atacttctgc tcttctgcct gcgcccagcg gtcagcttcc gtacgcttca gtacagcatg
     1441 ccatggtccc cagagggaga accagtcctt aaattcattc tcttcacggc ttctgaccat
     1501 ggcttcggca gtgcggaggt catttgctgt cactcccgac acgccataga aacgcatttc
     1561 cttcacggca gtggagagct gaagtttctc tgcgagcatg gtctggaagg ccaggtagac
     1621 ttctatctca tccacaaaat ggagagttct gactttatcc cgggcaatgt cctccagaat
     1681 ttcgaggcgg aacatttccc tgcccaggga gagcagagcg ccggtatcat tatcgaaaag
     1741 gccttctgat gcctgatgga ccaggagggt tttccggaga ttgttccatg tgagcgcgac
     1801 acggtcctca cagctctcag tggcatcagc agcaacagcg aaagactgct gtcgaagctc
     1861 cgcagaggca ctgagttttt ccagccatgc agcgacctgt tcacggaatc cggaggtatt
     1921 gcgtgcagag acggtatcgg aaaggcggtc aag
//
LOCUS       DAJTOR010000309         1934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTOR010000309 DAJTOR010000000
VERSION     DAJTOR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.316"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="OO249_004178"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="OO249_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5638245.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1149..1520)
                     /locus_tag="OO249_004179"
                     /pseudo
     CDS             complement(1149..1520)
                     /locus_tag="OO249_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1519..1934
                     /locus_tag="OO249_004180"
                     /pseudo
     CDS             1519..1934
                     /locus_tag="OO249_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          608 a          347 c          386 g          593 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc cttttggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ctagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac
     1261 gactggagtt gcttctgcgg tgctctggtg gcgaatgtga agtcatactc ttcgaacgtt
     1321 ttcaccgccg ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc
     1381 agtttttctt catgaagcag atgctccagg aagtccatat aactccattc ctggtctact
     1441 gcctgttgtg acagcgcagg cgctgcgctt atgtactgca cccattttgt tggacgatga
     1501 aatggaatag cccctaatat gtcaaagcca aaataccctt ttgaaaagcg ccttgaagtc
     1561 gtgaatcact acttcactac tgatgatggt tacaggatca tctcggcacg ttttggtgtc
     1621 cccgaaccca ggtcaggaca tgggttgccc tctatgaaaa acatggagaa aaaggcttaa
     1681 ttcccaaacc taaaggtgtt agtgctgatc cagagttgcg tattaaggtc gtgaaagctg
     1741 tgattgagca gcacatgtcc cttaatcagg ctgctgctca ctttatgctt gctggtagtg
     1801 gttctgtagc caggtggctg aaggtttatg aagagcgcgg agaagctggt ttacgcgcgc
     1861 tcaagattgg caccaaaaga aacattgcaa tatcagttga tccagaaaaa gcggcatcag
     1921 cattggagct gtca
//
LOCUS       DAJTOR010000310         1878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTOR010000310 DAJTOR010000000
VERSION     DAJTOR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.317"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(30..902)
                     /locus_tag="OO249_004181"
                     /pseudo
     CDS             complement(30..902)
                     /locus_tag="OO249_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(916..1056)
                     /locus_tag="OO249_004182"
     CDS             complement(916..1056)
                     /locus_tag="OO249_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638246.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVSVFNDNESKITDAAVNLFI
                     QI"
     gene            1195..1866
                     /gene="queE"
                     /locus_tag="OO249_004183"
     CDS             1195..1866
                     /gene="queE"
                     /locus_tag="OO249_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5638247.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          555 a          384 c          417 g          522 t
ORIGIN      
        1 cctccgggat ggcatttttt atggttgagt taccagcggc cggatgcgcc gccacctcca
       61 gaagaaccgc caccgccgct aaatccgccg ccacttgagc ctccaccgga accgctgttg
      121 tctgaatgat aacttttacc tgatgctctt ttctgaagta cacataaaca ggtaaagacc
      181 ataaatatgg tgaaagtaaa aaagaaggaa aatacagcaa tggaaaaaat tttatcggag
      241 aacaggaata aatctaaaag atagattgct gaaatacaga ctccactttt acatgcgcga
      301 caaaaattac tgccttgatg aaaaaaccag aaagggaaga acatcactgc aaatacccaa
      361 aaaatagcaa agtaataatg atcactggaa gacgctgatt cactttctga aggattagtc
      421 ggatattggt gttgtgaagt gagttggtta ttcaaagcgt ttatagctag ctctaatccc
      481 tgagcaaatt tttgttgttt aaatgcgggt atcatgttgc tacggatgat atctccagcc
      541 agagcatcgg ttaccttttc ttccagccca taacctacct ggatgcggac agtacgatct
      601 gaccaggcaa caatgatcag tatcccatca ttacgtttgg catctcctaa acgccaattg
      661 tcaaaaactc ttgtagcata ttgttcaatt gtctcgtctt tggttgtggg aacaattaat
      721 acagcaattt gcacttgggt attattgaaa atagattcta acttgtgcgt cagttctttc
      781 ttttcttgtg agtttaagct actcgtaaga tccgtaactt gttactgtaa ttgtgggaca
      841 atagttggtt gggctgcaac aaaggaacat acgaaagtga acattagtat gaaatagcgc
      901 attataaatt tctcattaaa tctggataaa taaattgacg gcggcatcgg taattttaga
      961 ctcgttatca ttaaaaactg atacagaaaa aataaatata attttggtga atgttttgac
     1021 atgattaaat ccattttctt tattttcttc tgacataata cgttactccc tttaattatt
     1081 aacgatatgt ttcgcatttt tctttaaatc tgcatgtaac agaatgttaa taaggcatga
     1141 ttgttgcggc aggatctgca ataattaaca attatccact tcacagagaa tgctatgcag
     1201 tacccgatta acgagatgtt ccagaccctg caaggtgagg gttactttac cggcgtcccc
     1261 gccattttta ttcgtttaca gggatgcccg gttggctgtg cctggtgcga caccaaacac
     1321 acctgggaaa agcttgagga tcgggaagtc tcccttttca gcattctggc gaagaccaaa
     1381 gagagtgata agtggggggc ggcgagcagt gaagatttgc tggctgtcat tggtcggcag
     1441 ggatataccg cgcggcatgt ggtgattacg ggtggtgagc cttgcattca tgatttgctg
     1501 ccactgactg acctgctcga aaagaacggt tttagctgcc agatcgaaac cagtggaact
     1561 catgaagtac gctgcacacc gaatacctgg gttaccgtat cgccaaagct gaacatgcgc
     1621 ggcggctatg acgtgttgtc acaggcactg gagcgagcca acgaaatcaa gcatccagtg
     1681 gggcgcgtac gcgatattga agcactcgat gaactattgg caacgctgac cgatgataaa
     1741 ccgcgagtca ttgcactgca gccgattagc caaaaggatg atgccacacg tttgtgcatt
     1801 gaaacctgca ttgcgcgtaa ttggcgtttg tcgatgcaaa cacataaata tctaaatatt
     1861 gcctgattaa acatttat
//
LOCUS       DAJTOR010000311         1855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTOR010000311 DAJTOR010000000
VERSION     DAJTOR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.318"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(20..>1855)
                     /gene="pdeF"
                     /locus_tag="OO249_004184"
     CDS             complement(20..>1855)
                     /gene="pdeF"
                     /locus_tag="OO249_004184"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5638248.1"
                     /translation="LISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNL
                     GTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLL
                     ALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIH
                     SYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVH
                     LPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLEPGE
                     DVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVN
                     HIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNHFFL
                     MAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTLQFM
                     AENRAKMPAHRFAINLSPTSVCQARFPFEVSQLLAKYQIEAWQLIFEVTESNALTNVK
                     QAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQI
                     VASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKP"
BASE COUNT          538 a          452 c          418 g          447 t
ORIGIN      
        1 tcaacgtatc aattaacggc tacggcttac caataagata accctgcata taatcgatcc
       61 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
      121 ttttcttcat tcgcgccagg tggcaaatcg atgccactat ctgataatcc agactatttg
      181 acacaatatt gcggataaaa ctgccgtcaa ttttaagcag atcggcattc acatttttaa
      241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
      301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
      361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
      421 caaaaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
      481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
      541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctatcg gggctgatca
      601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
      661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
      721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg cgacgctgca
      781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
      841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
      901 gcatcggcat gccatcccaa aagaaacgaa attgcttgag atggctatcc agtgcggtaa
      961 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
     1021 aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt tgcttgtatt
     1081 gaatccgcag catgatgcca tagttcttaa ccagcatttc catgccaggg atgcgtaaat
     1141 aacaaagcgc agaccagggg gcatcacgca acgcgcgatt cagggcgcga acatttggca
     1201 gatgaaccac cgggtccaca tacgcaagcc gctgaatgcg tctgactacc gctctctgac
     1261 gggttgccag tacagccata taattgacaa taaaagagaa taccagataa ctggaggagg
     1321 ttatggtcag ctgcgtggta tagccaggat aaatgggaat gtaattttga tagctgtgga
     1381 tgctgatcat caacacgacc gcccagagca gagaaatcag cttataacca tagcgcatcg
     1441 ctccccacat catcaggggc agcaataatg acaaggtata attggtgcta aaaattgtgc
     1501 ttttttcatt taacggcatg cataacagca acaataaagc acctaatgcc agtagccaga
     1561 gcgcgaactc ttttttggtg actttggcat caacctgctg ttttaattgc gaataatagc
     1621 tacgtaaata aaatggattt cgcactaccc gaatgatgaa gtagcacagc gggacaccga
     1681 tcagattacc caccagtaag gcctgataat tgattaaagt cccgaggtta aaaggcatga
     1741 caccgaccag attttctctg ctcgccaata atcctacaaa tgcagcaaac tggaaaagta
     1801 tcagaaacag cgttgcagga aacacaatct gccagaatat acgctgggaa attaa
//
LOCUS       DAJTOR010000312         1837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTOR010000312 DAJTOR010000000
VERSION     DAJTOR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.319"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>1837
                     /locus_tag="OO249_004185"
     CDS             <1..>1837
                     /locus_tag="OO249_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5638249.1"
                     /translation="RYEKGGKDGITMTFIGPSPSVQDKSASTDNTNINGEDMTEIEES
                     MLLPVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARAEELPAVTALAISHKTSLL
                     DSLEIRDLHKLVRDTDKVFPNPGNSDLGLITAFFEAYLDADYTDRGLLTKEWMKGNRV
                     SRITRTASGANAGGGNKTDRNPNLVHTLDTLDVEIAAATLPMDFNIYEIPGSVYRRAK
                     EVVLKKESPFKEWSAALRAPPGILDYSRAAIFALIRSAHPEFYHYPGRLQGYINAYLT
                     ETDHENPSKETLTATRHTPEKDILEEINREVVTERETEEEKPQPSDAMAGEQATTETM
                     EPDTTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIMQAANISQPDADKLLAVSRGEF
                     VEGISDPNDPKWVKGIQTRDSVNQNQHESERNDQKAEQNSPNALQNEPETKQSEPVAQ
                     QEPEKVCTACGQSGGGNYPDCGAVMDDATYQKTFDEENQVEVQENDPEEMEGAEHPHK
                     ENAGSAQDHASDSKTGETADPLITVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIIS
                     IGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREA"
BASE COUNT          561 a          465 c          448 g          363 t
ORIGIN      
        1 accgctatga aaaaggcggg aaagatggca tcacaatgac atttatcggc ccttccccct
       61 ctgttcagga caaatcagcc agcactgata ataccaacat caacggcgaa gacatgactg
      121 agattgagga gagcatgctt ctacctgtct ccggtcagga actgcccatt cgttggcttg
      181 ctcaacacgg cagcgaaaaa ccagtaacgc acgtttcacg cgacgaactc caggcattac
      241 acattgcacg ggctgaagaa ctaccggctg ttactgccct ggctatttcg cataaaacca
      301 gtctgctcga ctcgctggag attcgcgacc tccacaaact ggttcgtgac actgacaaag
      361 ttttccctaa tcctggtaat tcagacctgg gactaataac tgcttttttc gaagcatact
      421 tggacgctga ctacactgat cggggcctgc tgacaaaaga gtggatgaaa ggaaatcgtg
      481 tttcacgcat cacccgcacg gcttccggtg ctaatgctgg tggtgggaac aaaaccgatc
      541 gcaatccgaa tttagtacac accctcgaca cactggatgt ggagattgca gcagccacac
      601 ttccgatgga ttttaatatt tatgaaattc cgggcagcgt ttatcgtcgc gcaaaagaag
      661 tagtcctgaa aaaagaaagt ccgttcaaag aatggtccgc agcacttcgt gcacccccgg
      721 gtattctgga ctattcccgc gccgctattt ttgcacttat ccgaagcgca caccctgaat
      781 tttatcacta cccgggacgc cttcaggggt atatcaacgc ctatttgacg gaaactgatc
      841 acgagaaccc cagcaaggaa actctcacag ctacccggca tacgccggaa aaagatatcc
      901 tggaagaaat taaccgcgag gtggttactg agcgtgaaac agaagaagaa aaaccacaac
      961 catctgacgc aatggcaggt gaacaggcaa caactgaaac aatggaaccg gatacaactg
     1021 aacattgcca gaacgcgcag tcgctggatg ctcagtcgca ggtgagttcc gctaaccaag
     1081 taaaagtcac cgctgacgaa gtaaacaaaa ttatgcaggc agccaatatc agccagcctg
     1141 acgccgataa gttacttgct gtatcgcgtg gtgaatttgt tgaggggatt agcgacccta
     1201 atgatccgaa atgggtcaag gggatccaga ctcgcgattc tgtgaaccag aaccagcatg
     1261 aatcggaacg gaacgaccaa aaagcggaac aaaacagccc aaatgcgtta caaaacgagc
     1321 cagaaacgaa acaatccgaa ccagtagcgc aacaggaacc ggaaaaagtc tgcaccgcct
     1381 gcggtcagag cggtggcggc aactaccctg attgtggcgc ggtgatggac gacgcaacat
     1441 accagaaaac attcgatgaa gagaatcagg ttgaagttca ggaaaatgat ccggaggaaa
     1501 tggaaggcgc tgaacatcca cacaaggaga atgctggcag cgctcaggac cacgccagcg
     1561 atagtaaaac tggcgagacg gcagatccct taattacggt gaatggtcat cacgaaatca
     1621 catccaccag caggacgtgt gaccatctaa tgatcgacct tgaaaccatg ggaaaaaatc
     1681 ctgatgcccc gatcatctca ataggtgcaa tatttttcga tccgcaaacc ggagatatgg
     1741 gacccgaatt tagtaagact atcgatctgg aaactgctgg cggagtcatt gatcgggaca
     1801 ccattaaatg gtggcttaag caatcacgcg aagcgca
//
LOCUS       DAJTOR010000313         1807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTOR010000313 DAJTOR010000000
VERSION     DAJTOR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.320"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..158
                     /locus_tag="OO249_004186"
     CDS             <1..158
                     /locus_tag="OO249_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5638250.1"
                     /translation="IAPLQDAVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEW
                     PANPVRE"
     gene            273..443
                     /locus_tag="OO249_004187"
     CDS             273..443
                     /locus_tag="OO249_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638251.1"
                     /translation="MVRLPPVVMSAKSATARTTIARENAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(570..1127)
                     /locus_tag="OO249_004188"
     CDS             complement(570..1127)
                     /locus_tag="OO249_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5638252.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1124..1399)
                     /locus_tag="OO249_004189"
     CDS             complement(1124..1399)
                     /locus_tag="OO249_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5638253.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1585..1710
                     /locus_tag="OO249_004190"
                     /pseudo
     CDS             1585..1710
                     /locus_tag="OO249_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          506 a          377 c          439 g          485 t
ORIGIN      
        1 atattgcgcc actgcaggat gctgtagatc tggaaatcgc aacggaggaa gaaagatcgt
       61 tgctggaggc atggaataaa tatcgggtat tgttgaatcg tgttgataca tcaactgcac
      121 ctgatattga gtggcctgca aatcctgtca gggagtaatc attgggatta tgccgcagac
      181 acgtcgtatg caggaacgtg ctgcggttag tttgtgagct ttcgatagtg gttgttattt
      241 ttgcccttat ttgttccgga ggccatggtt caatggtccg tctgccccct gtggtgatgt
      301 cagcaaaatc agccactgcg cgaaccacaa tagcccggga aaatgctgaa gatcaccagg
      361 taaagctgtc agcgcagaaa ctggaagaac tgctcgcatc aatggttaag gatgaggttg
      421 atcgcaatga tgggatttat tgacgtcagc gagagcagaa ggaagaactg aataacctga
      481 atgatttacg ctcaatcaga gcgatggcga ttagcggcaa tcacgctcga taaaattaac
      541 cccggtgatc atccggggtt ttgcattcat taaaaccgca tcaacctttc caccaattgt
      601 tctttacggg caacgatcca cccatgttgc tccagataaa atttaaaccg ttccagcgtg
      661 cataccatcg catcggcggg gactttttct gtgaactcga cctgaccgtg tttatcgaag
      721 tggatcagta atgcgcatcc attatttggg gcgggggagt tttgtgctgc aggaggacgt
      781 ttgtggagtt cttcttccat tgcgttaaat ttttcaatgt aagcttcttt tagtctggct
      841 gcttttttgc cagtaaatcc cattacgagg aacatgaagc cgtcttttgt gatcaggtag
      901 catcgtgatt cacgttctgc gccgttgcca atttcgacag tctgaacatc ggccgaaaag
      961 ttggctgatg taaattcggg agagcagtcg agtgtgtcaa tcttagctaa aacgtgttta
     1021 tgttgtttat cgaagtagtc ggcaacagct tgagaggtgg taaccaggcg accatctttg
     1081 aaaaagactt ttggtgactc gttagaaaaa gttatagctg tattcatatg tgatgactcc
     1141 aattaattgg atgatcacca cctctgacgc caatcaggga ggtggtgaga cgtacagggt
     1201 tggcgtaacc ggtcatcaca aaacccggcg cggatttctc cgcccctgca cgccccacca
     1261 taatgcgaat gtggcaatgc ttaacgcaaa aaaaaccgca aacgcggcta tgcgttgtga
     1321 tgttatccga gacgccaatc ccgacaaccg attttgcggt tgtgtgagga atatagcccc
     1381 ggagaacaaa aactgtcaac gggcaaaaaa atttccgtta tcaggaatgt ataaaattaa
     1441 agcgatcgag caaaaaatcc tttctgcaaa actaaaaaag atttcaataa aatcaggcag
     1501 ttaaaaacac ggaaaaacgg tataaaaaag cgcctcaaaa tcctgttttt tctttcagat
     1561 acaatgtctt attgcatttt ttaaaacatg tcagtctggt acatggatat cgataccacc
     1621 gccaattata agctgggcgg tgcacagcaa catgatagcg tacgcctcga tccgtgggtg
     1681 tttatgttct cagcaggata tcgtttttaa ttccgcacaa aaacgacccc gtaatatacg
     1741 gggtcaataa ggacatggca taaagggtaa tgctgccaac ttactgattt agtgtatgat
     1801 ggtgatt
//
LOCUS       DAJTOR010000314         1765 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTOR010000314 DAJTOR010000000
VERSION     DAJTOR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.321"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..108
                     /locus_tag="OO249_004191"
     CDS             <1..108
                     /locus_tag="OO249_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5638254.1"
                     /translation="DASSVIHATGGDRVATSQTDRTAWHPDITRDKTRE"
     gene            105..1706
                     /locus_tag="OO249_004192"
     CDS             105..1706
                     /locus_tag="OO249_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5638255.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIP
                     LYRQSEIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVL
                     APGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADA
                     YAGYNVLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGS
                     PAEERLAVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDG
                     WVEIDNNIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLRE
                     VIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT          485 a          435 c          494 g          351 t
ORIGIN      
        1 gacgccagct cagttatcca tgctactgga ggggatcgcg tggcaacatc ccaaacggac
       61 agaacggcct ggcatccgga tataacccgt gataaaacaa gggaatgaac aatgaactcc
      121 ccgatgatat tgagctgctt aaagccatgt tgcgtaagca acagagtcgg cttcgacagt
      181 atgcctgtca ggtcgcgggc tatgagcagg aaattgaacg gctgaaagcg caactcgaca
      241 ggttgcgtcg tatgctgttc ggccagagtt cagagaaaaa gcgtcataag cttgaaaatc
      301 agatccgaca ggcagaaaaa cgactgtcgg aactggaaaa ccggctgaac acagccagaa
      361 atcttctgga agatgcatcg tcagtcacag attcacctga caccagtccc ccgtcagaaa
      421 acccgatcgc cagtaagcct gaatccccgg gacgaaaatc ttcacgaaaa ccgctgccgg
      481 cagaacttcc ccgggagaca catcgccttc tgcctgctga aaccagttgc ccggcctgtg
      541 gaggtgttct gaaagaaatg ggggaaacaa tctcagagca actggatatc attaataccg
      601 cctttaaagt tatcgaaacc atacgtccca aactggcctg tagccggtgt gatgtcatcg
      661 ttcaggcacc acttccccct aaaccgatcg aacgcggtta tgccagtgca gggttacttg
      721 cacggatcct ggtcagcaaa tatatggaac atatcccttt atatcgccag tcagaaatat
      781 acgcgcgaca gggcgtggag ctgagccgta ataccatggt gcgctgggta tcagaaatgg
      841 cagacaaact ccgtcctctg tatatagcgc tgaatgacta tgttctggag gcaggaaagg
      901 tgcacgcaga tgacactccg gtgaaagtac tggccccggg gaacggaaag acgaaaacgg
      961 gtcgtctgtg ggtatacgtc agggatgatc gtaatgcggg ttcatccctg ccggcagccg
     1021 tctggttcgc gtattcggca gatcgcaaag gagaacatcc gcagctccac ctggcaaagt
     1081 atcagggcgt actgcaggct gatgcctatg caggttataa cgtactgtac gaaacgggcc
     1141 gggtgaagga agccgggtgc ctggcccacg cccgccgaaa aatccatgac gaggatgtgc
     1201 gccgtccgac agaaatgact caggaagcgc tcagacggat agcagagtta tacgacatag
     1261 aagcggagat acgtggcagt ccggcagagg aacggcttgc agtcagaaaa gccagaagcg
     1321 tccagttgat gcagtcattg tacgactgga tacagttgca gaggaaaacg ctgtcgaaac
     1381 atgcggagat ggcgaaggcg ttcgactata tcctgaatca ctggaatgcg ctgaacgagt
     1441 tctgtcgtga cggctgggtg gaaatagaca acaacatcgg tgaaaacgcg ttacgatcgg
     1501 tggcggttgg aagaaaaaat tatctctttt tcggctcaga caagggagga gaaagtgcgg
     1561 cgatcatcta cagtctgctg gtcacctgca aacagaacga agtggagccg gaggactggt
     1621 tgcgcgaagt gatcgagaag ctcaatgact ggccgtcgaa ccaagtgcat gaactgctgc
     1681 cctggaactt ctcgtctgta aaataatcct tacgctacgt acttctcggg gcgcttacac
     1741 cgttactgtt cattgtccgc gctgt
//
LOCUS       DAJTOR010000315         1720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTOR010000315 DAJTOR010000000
VERSION     DAJTOR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.322"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..171)
                     /locus_tag="OO249_004193"
     CDS             complement(<1..171)
                     /locus_tag="OO249_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR5638256.1"
                     /translation="MTIEKSRFSSGAAPQPNVNTGVINGNDFAAIVPVIAGRIGGREA
                     NIVSAKALHKALG"
     gene            complement(185..616)
                     /locus_tag="OO249_004194"
     CDS             complement(185..616)
                     /locus_tag="OO249_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638257.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYASELERRLDIQLERIDEKNPDGIGSHLRYRFTGR
                     GDVLKVIRLVNHNADAGGYQGLSQPEIADILNLYPDNLTAA"
     gene            complement(616..819)
                     /locus_tag="OO249_004195"
     CDS             complement(616..819)
                     /locus_tag="OO249_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR5638258.1"
                     /translation="MNIDTIPTKGYIRRFRLAELLGVSVSTIDRKVRNGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGKVA"
     gene            complement(932..>1720)
                     /locus_tag="OO249_004196"
     CDS             complement(932..>1720)
                     /locus_tag="OO249_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638259.1"
                     /translation="IDIDDLLIMAAAESIYLSAYLCGYDGTLHSIASLYRNCSLFPDV
                     KEDCLLLADIITEVEDEKSFKAGVVNHIDCHVLGLWHVNASYILDIAGLDSIDTPARI
                     FSPAHRKRELDVSANYYFFLDYTKHAFHEISVEDLYITRPWVEYIGECMDKNQPIYSP
                     ETRINLQALYEDVKNVEHGNRERHRQKREICLLALIYVKRHYPEECKGKNGKETNEAW
                     ANATINHWALLGGGYDEPSIDYLKKLIADADRTPEERTTAGKKR"
BASE COUNT          385 a          415 c          404 g          516 t
ORIGIN      
        1 ccccagcgct ttatgcaacg ccttagcgct cacaatattg gcttcacgcc cgccaatgcg
       61 tccggcaata acgggaacaa tagcggcaaa gtcgttgccg ttaattactc ccgtgttaac
      121 gttgggttgc ggggcggccc ccgaactgaa tcggcttttt tcgatagtca tattttcggc
      181 tccgttatgc ggcggtgaga ttgtccgggt atagattgag aatgtcggca atttcaggct
      241 gagaaagtcc ctgatagccg ccagcgtcgg cgttatggtt cacaagccga ataactttta
      301 gaacgtcacc gcgcccggta aagcgatagc gtaaatgtga ccctatcccg tcggggttct
      361 tttcgtctat acgctcaagc tgaatatcaa gcctgcgctc taactcgctg gcgtagttcc
      421 ttccagacga aaggcggcaa taacgcagaa tgtcattttc tgtccatccc tctataccag
      481 tacgcagcat atagactctg gcacgatgct ttttcggtgt gcgctttggt gcttgaaggg
      541 attttgtaag gggcgtaaca tcagatacgc gaatatctga attagccgcc tgtaatgggc
      601 ggtttttctt ttccatcagg ccaccttccc gcggcgttct gccagccagt tgtgaatctc
      661 aaccgcatca aacgcagtta ttttttctcc cagctttacc ggacgtggta gtgaaccgtt
      721 tcttacttta cggtcgatgg tggatacact aacgcccagc aattcagcca ggcggaagcg
      781 tctgatatag ccttttgtag ggatagtgtc gatgttcatg attcccgtaa cctcttagcg
      841 atagttgttg cttcgttacg ggtatttaat ggcgtgcacg ctcaaaaagg aatgatcgta
      901 ttttttacta tcgtgatagt aaaatttact atcacctttt tttccctgcc gtggtgcgtt
      961 cttccggtgt tctgtctgcg tcggctatga gtttcttcag gtaatctatg gatggctcat
     1021 catagccgcc ccccaaaagc gcccagtggt ttattgtggc gttcgcccat gcttcgttgg
     1081 tttccttccc gttcttccct ttacactctt ctgggtagtg ccgcttcaca taaatcaacg
     1141 ccaagaggca aatttcgcgc ttttgcctgt ggcgctctct gtttccgtgc tcaacgtttt
     1201 taacatcctc ataaagagct tgtaagttta tgcgagtttc ggggctgtat atgggctggt
     1261 ttttgtccat gcattcgccg atatattcaa cccacggcct tgttatgtac aaatcctcta
     1321 cggatatttc gtggaaggca tgttttgtat aatcgagaaa aaaataataa ttcgcactta
     1381 cgtctagttc tctttttctg tgtgcggggc tgaatatgcg tgcgggagtg tcaatagaat
     1441 caagccctgc gatgtcgagt atataggagg cgttaacatg ccatagccca agcacatggc
     1501 aatctatatg atttacaacg cctgccttaa agcttttttc gtcctctact tcagtgatta
     1561 tgtcggcaag taataagcag tcttctttga cgtctgggaa taaagagcaa ttgcggtaaa
     1621 gactcgcgat gctatgtaat gtgccgtcgt agccgcaaag gtacgcgctc aaatatatag
     1681 attctgccgc cgccataatc aaaagatcat caatatctat
//
LOCUS       DAJTOR010000316         1616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTOR010000316 DAJTOR010000000
VERSION     DAJTOR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.325"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..350)
                     /locus_tag="OO249_004197"
     CDS             complement(<1..350)
                     /locus_tag="OO249_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR5638260.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRN"
     gene            592..885
                     /locus_tag="OO249_004198"
     CDS             592..885
                     /locus_tag="OO249_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5638261.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            957..1616
                     /locus_tag="OO249_004199"
     CDS             957..1616
                     /locus_tag="OO249_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5638262.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
BASE COUNT          418 a          351 c          421 g          426 t
ORIGIN      
        1 tcgtttcggg tttgcagcac tcgcagctca tatttgccag aagcagggag tgagtattgt
       61 ccgtgactat cgagttctgg ggaatatctg gataaatcaa cagaatcatc gattcctggc
      121 ccgaagaggt aagtatcagc gccttcatta gagatactca catgtacttt ctgacctttt
      181 ttggcataga aggtatatgt atcgtaatcg tatcccttta tttcgccgga gtactgagcg
      241 ctgctatggc cttttctgaa ctcaacattg acattcttac ccgcagcaaa gctcgtggag
      301 gttagcagtg caaacagcaa tacagcctta ctgatgctct tgattttcat tggtagtctc
      361 ctgttcagaa tgagtacgat gaactgttat aatataacaa tccctaacgg gagaattcca
      421 gtggcttttc tgcgttcggt aaaatcccgg cgcggcattt tttgccggtg ggattactta
      481 taaaggggca tggactcaac aaaccgcaga aatctacagc tggctggctt gtggcgcggg
      541 tttcattgat gttagcttat gccaaaacct ggcatcttaa aaagtaaaag tatgttttgt
      601 gtgatttatc gaagcagcaa gcgtgaccag acctatttat atgtcgaaaa aaaagacgat
      661 ttttcgcgtg ttcctgagga actgatgaaa ggttttggtc agcctcagtt agcgatgatt
      721 ctgccgttgg atgggcggaa gaaactggtc aatgctgata ttgaaaaggt taaactggca
      781 ttaaccgagc aaggttacta tttgcagtta ccgccaccac ccgaagattt gctgaagcaa
      841 catctttccg tcatggggca gaaaacagac gacacgaaca aataaccgat atccggcggt
      901 ggcattatct ttgtcggcgc ggcttttcat atccacgata aggtgagggg aacgttatgt
      961 atcaacatca caactggcaa ggtgcgctgc tggattatcc ggtgagtaaa gtagtctgtg
     1021 ttggcagtaa ctatgccaaa catattaaag agatgggcag cgcagtgccc gaagagccag
     1081 tgctgtttat taaaccagaa acggcactgt gcgatctgag gcagccattg gcgatcccat
     1141 ccgatttcgg ttcagttcat catgaagtcg aactggcggt gttgattggt gcgacgctgc
     1201 gtcaggcaac ggaagagcat gtccgcaaag ccattgccgg ttacggcgtg gcgcttgatc
     1261 tgacattgcg tgatgttcag ggaaaaatga agaaagccgg gcagccgtgg gaaaaggcta
     1321 aagcgtttga taactcttgt ccgctttccg ggtttatccc cgcggcggaa tttaccggcg
     1381 atccacaaaa tacaacgctg ggcctgagcg taaacggcga acaacgccag caaggtacga
     1441 ctgcggacat gatccataaa atcgttccgt tgatcgctta tatgagcaag ttttttaccc
     1501 tcaaggccgg tgacgttgtg ttgacaggca cgcctgatgg cgtcggcccg ttgcaaagcg
     1561 gtgatgagct gacagtcact ttcgatgggc attctttgac aactcgcgtt ttgtaa
//
LOCUS       DAJTOR010000317         1609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTOR010000317 DAJTOR010000000
VERSION     DAJTOR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.326"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..1606)
                     /locus_tag="OO249_004200"
                     /pseudo
     CDS             complement(1..1606)
                     /locus_tag="OO249_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441428.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          418 a          409 c          349 g          433 t
ORIGIN      
        1 aaaaaggcaa acagatccga agcgggataa tctttttggg gagaattgtt ccagttagca
       61 atatatcccg acagggggtt atacacctta gggttcattt caaaaggcaa tagccctttc
      121 cagtcccatt ttcccgtacc aggaacgggt aatcgcggat catggcctga ttgacgatct
      181 ggataagcac cagtatgaac ataaccaata ttgccgttta catcagcata gtaccagttg
      241 atggtcagtg cttgtttcgc tactcctgcc aatttttggc cttcatctga tgagtccagg
      301 ccagcaaaga cgccacctct ttaccatccc atgcgcggga tttagcgtaa gccgtttgtg
      361 tcgtctggtc agtttggaga atgttgccat gcaccgtacg ccagacagta aaggtctctg
      421 cctgaccatt tttcaccgta atggtttcct cacggcttaa cattttcacc cacttacaat
      481 tatgtaagta gtagcctggt ttctctgccg acagccgttc agcaaaaata tcgacatcat
      541 cgccgaaacc tgccgttgat ccccaggaaa tcacaccatt atgaccaaaa accagcccag
      601 gataggcaaa tggtgtattg ccagtgacat cataaccagc accgtgcaga ccaataccat
      661 aagtatacgc aggcgcatac cagccaaact gcggaccatt taccatgatt gctttcgcat
      721 cctgggcttt gcttttgccg atcacccaca tattgctggt cgttggatac cccgccagac
      781 cattggcacc accctgtgca aattgtgcag caatagtttc ccggttcttc cctgctgtta
      841 acgccagcag tgcgccatcc gccccttttg ctggtcggtc aagcattggt gcaggtaaat
      901 cgtagcgtgg caacagagct gctgtttgcg agttttgctg attaaattta agtgggtagc
      961 tactctcttg tacggcaata gtggttggcg ctgatgggtt taccagccat ttcaactgat
     1021 taaataccgc catgccttgt gatacaccat atttatcttt taaagccgtt agcagtgcca
     1081 gattatcaat ttcgctagtg ctatcagaga agcggtttgc catggtgccc acaaatatca
     1141 tcgcgacatc aaacggttcc cagcgcttag gagtaaagcc aaatgtatta aactgttttg
     1201 gtaagagcgt ctctggattg gtatttacct tatcaatcca ggcattcatt ccatcagcgt
     1261 agccttgcag aatggacata tcctctgggg aaagggcatc aatttgcgcc cggatagcat
     1321 ccggccagta gttacgacgg atatctttat caaatttcac aaaatctttg ccaagcactt
     1381 ccgcgacagt cccttgagta ctgcgacgtg ccatttccat ctgaaaaagg cgatcttgtg
     1441 ctactacata gccatagcca taaaataggt gccatgtatc attggcataa atatgcggca
     1501 tgccgtattc atcgcgaaca atctttatct cacttgacga ctgctcagcc agtgcaggta
     1561 agctccaata atacatcagg gaagcagtaa cacagttcac gatcatacg
//
LOCUS       DAJTOR010000318         1589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTOR010000318 DAJTOR010000000
VERSION     DAJTOR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.327"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..597
                     /gene="argO"
                     /locus_tag="OO249_004201"
     CDS             <1..597
                     /gene="argO"
                     /locus_tag="OO249_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5638263.1"
                     /translation="AMILPLGPQNAFVMNQGIRRQYHIMIALLCAISDLVLICAGIFG
                     GSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAEVLKQGRWKIIATM
                     LAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWFFGLAILAAWLAPR
                     LRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            690..1430
                     /locus_tag="OO249_004202"
     CDS             690..1430
                     /locus_tag="OO249_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5638264.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
BASE COUNT          375 a          373 c          437 g          404 t
ORIGIN      
        1 gctatgattc tgccgctcgg cccacaaaat gcttttgtga tgaatcaggg cattcgtcgt
       61 cagtaccaca ttatgattgc cttactttgc gctatcagcg atttggtcct gatttgcgcg
      121 gggatttttg gtggtagcgc gttattgatg cagtcgccgt ggttgctggc gctggtcacc
      181 tggggcggcg tagtcttctt gctctggtat ggttttggcg cttttaaaac agcgatgagt
      241 agtaatattg agttagctag tgccgaagtc ctgaagcaag gcagatggaa aattatcgcc
      301 accatgttgg cagtgacctg gctgaatccg catgtttacc tggatacttt tgttgtactg
      361 ggcagccttg gcgggcagct tgacgtcgaa ccaaaacgtt ggtttgcgct cgggacaatt
      421 agcgcctctt tcctgtggtt ctttggtctg gctattctcg cagcctggct ggcaccgcgc
      481 ctgcgcacgg caaaatcaca gcgcattatc aatctggtag tgggatgtgt catgtggttt
      541 atagccttgc agctggcgag agacggtatt gctcatgcac aagccttgtt cagttaggcg
      601 ctatctgatg gaaaaataaa acagaggcgc taagcttgcc tccagaggtc ctgaattttg
      661 ggcaagtcaa ttaacacgga gagactaacg tgaagttcaa agttatcgcc ctagcggcat
      721 taatgggtat tagcgggatg gcagcgcagg ctaacgaatt gccggatgga ccgcatattg
      781 tcacctccgg tacggcaagt gtggatgcgg tgccagacat tgccactctt gcgattgaag
      841 ttaacgtggc cgcgaaggat gccgctacag ccaagaaaca ggcagatgag cgcgtcgcac
      901 aatacatttc cttccttgaa ctcaatcaga tcgcgaaaaa agatatcagc tcagcgaatt
      961 tacgcaccca gccagattat gattatcagg atggtaaaag tatcctgaaa ggctaccgcg
     1021 ctgtgagaac ggtggaagtc acgctccgtc agttagacaa actgaattcc ttgctggatg
     1081 gcgcgctgaa ggcgggtctt aacgaaattc gttcagtgtc gctgggcgtg gcgcagccgg
     1141 atgcctataa agacaaagcg cgtaaggcag cgattgataa cgcgattcat caggcgcagg
     1201 aactggcgaa cggctttcat cgtaaactgg ggccggtata tagcgtgcgc taccatgttt
     1261 ccaactatca gcccagccca atggtgcgga tgatgaaagc cgatgccgcg ccggtgtctg
     1321 cccaggaaac ttacgagcag gccgctattc agtttgatga tcaggtcgat gtggtcttcc
     1381 agttagaacc tgtggatcaa caacccgcta aaacacctgc agcacaataa tacttgtagc
     1441 ccgacgtatt cgtcgggcta atgcctttac ccgatattgc ctcctttttc tgtaattttc
     1501 tgtaaaacca tgacgtaatc acctatcttt gctcgcggtt tcatcaagga taggctgact
     1561 gaaacggtta agcggacaag gatgcgggg
//
LOCUS       DAJTOR010000319         1517 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTOR010000319 DAJTOR010000000
VERSION     DAJTOR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.328"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..1386)
                     /locus_tag="OO249_004203"
                     /pseudo
     CDS             complement(1..1386)
                     /locus_tag="OO249_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          519 a          262 c          224 g          512 t
ORIGIN      
        1 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
       61 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
      121 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
      181 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
      241 tatttttatc aatcagccct ttattcatat taaacctgtt aataaaatgg atcatctgtt
      301 gggtatcgaa atccaccttg ctgaatcgat gttgacgcat aaaatcatcg aacttgttat
      361 cttcaccaga atggatataa ctgcgtataa ttggatcttt tatataagga caatggctaa
      421 atgttttctt catattctca ataaagtttt tattaacaat attaagaatt tttaattgca
      481 taaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
      541 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
      601 aatcaatagt taattccaat tgttctgtaa tgcaatcttt aaaattccct gtcgacattc
      661 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
      721 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
      781 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
      841 tatcaagaat atcttggtta aaatcaacat attcgccact cgttttgaga aaaatcacat
      901 tgtgtaaacg tcgacattta aatatgacat tatcaactgt tgccttagta aaattgcaat
      961 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
     1021 tagaaataac gtcaccttta aaatgacaat ttgttacatc aaaatcagac aggtcgatat
     1081 acccctgcga atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
     1141 agaattttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
     1201 gattttttcg ttccgttgtt ttatatttaa aaatatatta ctgatataaa taaagttatc
     1261 tctgtttaaa cctgtcaccc cattgaaatt acgtagtaaa tcaagatcta ctatattatt
     1321 tccttgaacc acatcatgtt tcatcaaaga gttattgcca ccagaatttg tgatatgacc
     1381 tatcataaaa cctctgtaat acagaaataa aataacctgg aaaagtagcc tgagcgtcca
     1441 gacactaccg gcgaaaggaa aagaatctct gataaggcat tgagataatg gacattctta
     1501 gtgaagaaga agtgaag
//
LOCUS       DAJTOR010000320         1514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTOR010000320 DAJTOR010000000
VERSION     DAJTOR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.329"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1495
                     /locus_tag="OO249_004204"
     CDS             <1..1495
                     /locus_tag="OO249_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCR5638265.1"
                     /translation="RADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIALVKDA
                     KKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIESGIPGYWQGQVLIPFI
                     KDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWENLHALKEKRLNGMPKTTPKSGQ
                     VIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSIIPPLKNYNERLHNTNLFNPKY
                     QKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLQTLAS
                     KPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLV
                     YWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEG
                     VFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIYKNMGSNSVN
                     IIINNTRKIIPPGKVFTLRGGTLNINIPGRSALLLGKTGEPPNYLYL"
BASE COUNT          524 a          287 c          291 g          412 t
ORIGIN      
        1 ccgcgccgac ggaccagaaa ctcccgaaga agaaaaaggt tactggtttc atgcttatca
       61 acccgaagat ctttgtacca tccataaccc aatgggagat ttgcaggatt ttatcgcatt
      121 ggttaaagat gctaaaaaat ttggtatcga tatcattcct gattatacat ttaactttat
      181 gggaatcggg ggtagtggta aaaatgacct ggattatccc tctgctgata tacgagcgaa
      241 gatcagtaaa gatatagaaa gcggtatccc tggctattgg caaggtcagg ttttgattcc
      301 attcattaaa gatccagtaa caaaagaacg taaacaaatc catccagaag atatacatct
      361 cactgcaaaa gacttcgaag caagtaaaga taacatctct aaggatgaat gggaaaacct
      421 ccatgcatta aaagaaaagc gtttaaatgg aatgcctaaa acaacaccca aaagtggcca
      481 ggttattatg ttgcaaaatc aatacgttcg agaaatgcga aaatatggcg tacgaggttt
      541 acgttatgat gcggcaaaac actcaaaaca tgaacaaata gagaggtcga taatcccacc
      601 gcttaaaaat tataatgagc ggttacacaa tactaattta tttaacccaa aatatcaaaa
      661 aaaagccgtt atgaattaca tggaatacct ggtaacttgt cagttggatg aacaacaaat
      721 gtcatcactg ctttatgaga gagatgattt aagtgccatt gatttttctt tactcatgaa
      781 aacgataaaa gccttttcat ttggtggaga tctccaaacc cttgcatcaa aaccgggttc
      841 cacaatctca agtatcccat cagaaagacg gatattgatt aacattaacc acgattttcc
      901 taacaatggt aatcttttca atgactttct atttaaccat caacaagatg aacaattagc
      961 aatggcatat atggccgctc tcccgttcag cagaccttta gtttactggg atggccaagt
     1021 attaaaatca acgactgaaa ttaaaaatta tgatgggtcc acgcgtgtcg gcggtgaggc
     1081 gtggcttaat aaaggttgct ctacctatca gcagctctac aatgaattcc acgcattata
     1141 tatagataaa gcaggaatat ggagcgcatt tgagggtgta tttgcaacta aaaacgttct
     1201 ggcctttagt cgtggggatt ctgtgaacat taatcactct cctcatgatg ggctagttat
     1261 aataaataaa ggaaacgaag aagttgaagg tacctggcct aacaaattgc aacctggaat
     1321 atacaaaaac atggggagta atagcgttaa cattattatt aataataccc gaaaaattat
     1381 cccccctggt aaagtattta cgcttagagg cggaactcta aatatcaata ttcctgggcg
     1441 tagcgctctt cttttaggga aaacaggaga accgccgaac tatctctatt tataatttaa
     1501 tgttatatct gccc
//
LOCUS       DAJTOR010000321         1514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTOR010000321 DAJTOR010000000
VERSION     DAJTOR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.330"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(61..339)
                     /locus_tag="OO249_004205"
     CDS             complement(61..339)
                     /locus_tag="OO249_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638266.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            280..465
                     /locus_tag="OO249_004206"
     CDS             280..465
                     /locus_tag="OO249_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638267.1"
                     /translation="MPAGRRGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(507..662)
                     /gene="hokD"
                     /locus_tag="OO249_004207"
     CDS             complement(507..662)
                     /gene="hokD"
                     /locus_tag="OO249_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5638268.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          385 a          374 c          343 g          412 t
ORIGIN      
        1 ggcttaagga ccaccagccc gagttccgga accagaacag gtcgaagtaa tacccgcaca
       61 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
      121 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      181 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      241 ctgataccag tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggcgcggc
      301 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      361 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      421 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      481 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      541 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      601 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      661 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      721 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtagtgttaa ctggggcttt
      781 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      841 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      901 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      961 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
     1021 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1081 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1141 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1201 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1261 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1321 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1381 tcgcgtttta gttgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1441 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1501 tttttcggta cata
//
LOCUS       DAJTOR010000322         1513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTOR010000322 DAJTOR010000000
VERSION     DAJTOR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.331"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..876
                     /gene="lpxM"
                     /locus_tag="OO249_004208"
                     /gene_synonym="msbB"
     CDS             <1..876
                     /gene="lpxM"
                     /locus_tag="OO249_004208"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5638269.1"
                     /translation="TWFLLGMIAGISMFPPSFRDPVLAKIGRWAGRLSKKARRRATIN
                     LSLCFPEKSDTEREIIVDKMFATALQSIVMMAELAIRGPEKFQKRVFWKGLEILEEIR
                     YNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFHQQRNPVIDYVWNSVRRKFGGRLH
                     SREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAM
                     IIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMIARQMNKTVEILVGSHPEQYIWVL
                     KLLKTRKSNEADPYP"
     gene            complement(1030..>1513)
                     /locus_tag="OO249_004209"
     CDS             complement(1030..>1513)
                     /locus_tag="OO249_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5638270.1"
                     /translation="DIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVS
                     YVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRVIY
                     QPVYSPWVNHAERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV
                     "
BASE COUNT          386 a          335 c          366 g          426 t
ORIGIN      
        1 acatggttcc ttttgggaat gattgcaggt atttcaatgt ttcccccttc attcagagat
       61 cctgtcttgg caaaaatagg gcgttgggcg ggcagattga gcaaaaaagc tcgtcgcagg
      121 gcgacgatta atttatcgct ttgtttccca gaaaagagtg atacagaacg ggaaataatt
      181 gtcgacaaaa tgtttgccac agcattgcaa tccatagtga tgatggcaga actggcaatt
      241 cgtggtccgg aaaagttcca gaaacgagtg ttctggaaag gcctcgaaat tcttgaggaa
      301 attcgataca ataacagaaa tgtgattttt ctggttcccc atggctggag cgtggatatt
      361 cctgcaatgt tgctggcagc tcagggggaa aaaatggccg ccatgtttca tcagcaacga
      421 aatccagtga ttgattatgt ctggaattct gtacggcgta aattcggggg gcgcttacat
      481 tcccgggagg atgggataaa accatttatt cagtcagtac gccagggata ctgggggtat
      541 taccttccag atcaggatca tggtcctgaa tacagtgaat ttgctgattt ttttgcgacc
      601 tataaagcga cattaccaat tattggacgt ctgatgaaca tcagtcaggc tatgattata
      661 ccgcttttcc cggtttatga tgaaaaaaaa catttcctga ctattgaagt tcggccacca
      721 atggatgcat gcattgccag cgcggacaat aaaatgattg cccgacaaat gaacaaaaca
      781 gtggaaattt tggtggggtc acatccggaa cagtatatct gggttttaaa attgttaaaa
      841 acgcgcaaat caaacgaagc ggacccgtac ccttgaaatt ataaagtaca ggtatttcca
      901 ctagttgttt cttacaggtt accaatcgaa acacatcccc cttccgtgtg ttgcccgttt
      961 ggcacggagg actgtgtaga tgcagttcgt taaatttaat ttatgcctaa atagctgcgc
     1021 ttaataccgc tacacttttg ccagtccatg tttgcctccg gggaatgggc tgacggtttc
     1081 cataaaatgg cgaacttttt tcaacagttg ccacattgag cggcactgat gattacgcgt
     1141 tattgtgtcg tgaagtgcct gccatagccg ttcagcatga ttcacccatg gcgagtaaac
     1201 cggctgataa atgaccctga acttcgggtt ctccttcagc cagctctgtg tttcccggct
     1261 tttgtggata atgtagttgt ccacgatcag cgtgatggtt ttcgcccgac ggtatgtcgc
     1321 tttaagccgc ttcagcaggc tgatgaacag cgccgaactt ttgctgttgc cgcccacata
     1381 gctgacttta cctgtcccgc tgtgcagcgc tccggccaga taatattttt cattctgtcc
     1441 cggcgtgacc acccgttttt gctgtccgcg cagttgccag tccgcaccga ttttgggatg
     1501 aagatggata tcc
//
LOCUS       DAJTOR010000323         1475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTOR010000323 DAJTOR010000000
VERSION     DAJTOR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.332"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..730)
                     /gene="pdeC"
                     /locus_tag="OO249_004210"
     CDS             complement(<1..730)
                     /gene="pdeC"
                     /locus_tag="OO249_004210"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR5638271.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFSSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHL"
     gene            1160..1441
                     /locus_tag="OO249_004211"
     CDS             1160..1441
                     /locus_tag="OO249_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5638272.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          425 a          324 c          330 g          396 t
ORIGIN      
        1 taagatgatt ataaaaatgg gttataagac gttgatatga agtcgcaaca atggctgcaa
       61 ttgggcgttt tgtcgaataa actgtcgcat ataaatagcc atttctttgt acggttaaat
      121 ccttcaaatg aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt aacgagaaaa
      181 aggttttcgt caccgtatca tacacacccc attgcaatgt cggatcatca gacatcactt
      241 cactccagaa gagagggttg ataaccgcca cataatttcc ccgctgcata taggtcattt
      301 tatagccaga ggaaaaaggc gtatcgcggt aataatagat agaaacgtta ggttcacgct
      361 tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat gagcataaaa
      421 aatggttatc acgggcatag atcaattcat taatataaag atagccacga ataatattca
      481 acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc
      541 tcactaaatc tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg
      601 ttcggagctg attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa
      661 tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac
      721 gatgactcat tgctatcaat ttcctgcctg tttatacccg gccattgtaa ggtgaaaacc
      781 ctgggtaccg acgtctttag ataaatctta cggtataaat agcacttttg cctgatttga
      841 aattgatacc tttctaatcg tcacctggat gaaaataatt cattgatatt caaaattaat
      901 ttcctacagg aatgcagagg cggcgggaag atatgccctt aatgccactg aaaaaccaca
      961 taatctgtac agcaataaaa gtcacggcct aatatggcta acaaacaatc aattgtgata
     1021 tatttcacaa aattaatgaa acaaacagag tgtttcattt ttgtgttctg caagccccgc
     1081 gatagccaga gtgtaccacg cctcccgtga acaacgccgc gctgtccagg gcatcggctc
     1141 ttttcagagg aattgtttga tggctaccct caccactggc gtggttcttc ttcgctggca
     1201 acttcttagt gccgtaatga tgtttctggc cagcacactc aacatccgtt ttcgtcggtc
     1261 tgattatgtc gggcttgcgg tgatcagcag cggtctgggc gttgtttccg cctgttggtt
     1321 cgcaatgggg ttgcttggca tcacaatggc ggacatcacc gctatctggc acaacatcga
     1381 gtcggtgatg atagaagaga tgaatcagac accgccacaa tggccaatga ttttgacctg
     1441 atacgtagaa gcctccataa cggagtaatg ccgat
//
LOCUS       DAJTOR010000324         1460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTOR010000324 DAJTOR010000000
VERSION     DAJTOR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.333"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..537
                     /locus_tag="OO249_004212"
     CDS             <1..537
                     /locus_tag="OO249_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5638273.1"
                     /translation="EPGSITVPFTATPPVSRIDAKRGQTIKLMYTASTSLPKDRESVF
                     WFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPLALKWFWSGSE
                     GKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKASSAKV
                     HFYAINDFGGTIEGNASL"
     gene            572..1460
                     /locus_tag="OO249_004213"
                     /pseudo
     CDS             572..1460
                     /locus_tag="OO249_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          423 a          320 c          316 g          401 t
ORIGIN      
        1 gagcctggca gtattacagt cccttttact gccacgccgc cagtatcgcg tattgatgcc
       61 aaacgtgggc aaacaatcaa attaatgtac acagccagca cctcactgcc taaagacaga
      121 gagagcgtgt tctggtttaa cgtactggaa gttccaccaa aaccagatgc agaaaaggtc
      181 gcgaatcaaa gtctgctgca actggcattt cgcacacgta taaaactttt ctatcgcccg
      241 gatggattga agggaaatcc ctctgaagcc ccgttagccc tgaagtggtt ctggtcaggt
      301 tcagaaggta aggcgtcatt gcgagtgacc aatccaaccc cttactacgt ctcttttagc
      361 agtggtgatt tagaagctag cggtaaacgc tatccgattg atgtgaaaat gattgcacca
      421 tttagtgatg aggtcatgaa agtcactggc cttaatggca aagcaagttc tgcaaaagta
      481 catttttacg ccattaatga ttttggtggc accattgaag gtaatgccag tctgtaatca
      541 ggcaaggata taattccgca ggaagcataa cgtgactata aaaccaacta aaaattatca
      601 taatcatctg acccgtatcg ctacgttttg cgcgctgctg tattgcaata gtgctttctg
      661 tgctgaactt gttgaatatg accatacctt cctgatgggg cagaatgcct caaatattga
      721 tctcagccgg tatagtgaag gtaaccctgc tataccgggt atgtacgacg tcagtgttta
      781 tgtcaacgat caacctatta ttaaccaaag cattacattt attgaaattg aaggaaaaaa
      841 aacgcacaag cctgtatcac acttaagaat ttattgcagt ttcatataaa ttctcccgat
      901 ataaataacg aaaaagccgt tctgcttgcc agggatgaaa cactcggcaa ttgccttaat
      961 ttgacggaaa ttatccctca ggcttctgtt cgttatgacg ttaacgagca acgtctggat
     1021 atagacgttc ctcaggcatg ggtaatgaaa aattaccaaa actatgttga tccatcgtta
     1081 tgggaaaatg gcattaatgc ggccatgttg tcatacaacc tcaacggata tcatagtgaa
     1141 actcctggtc gaagaaatga cagcatttat gctgctttta acggtgggat gaatttaggt
     1201 gcatggcgac tgcgtgcctc aggcaactac aactggatga ccgattctgg cagtaattat
     1261 gattttaaga atcggtatat acagcgtgat atcgcctcgc tgcgttctca actcattctt
     1321 ggtgagtctt atacaacagg cgaaaccttt gattctgtca gtatccgcgg cattcgttta
     1381 tacagcgaca gccgcatgtt gcctccgaca ttagcgagtt ttgcgcctat cattcatggc
     1441 gttgctaata ccaacgccaa
//
LOCUS       DAJTOR010000325         1455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTOR010000325 DAJTOR010000000
VERSION     DAJTOR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.334"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            95..694
                     /locus_tag="OO249_004214"
     CDS             95..694
                     /locus_tag="OO249_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5638274.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            758..>1455
                     /locus_tag="OO249_004215"
     CDS             758..>1455
                     /locus_tag="OO249_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5638275.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVVQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAENARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAG
                     EKAQQKSRDNLGLK"
BASE COUNT          351 a          347 c          459 g          298 t
ORIGIN      
        1 gccactgcag gcatcacgat tagctgaatt ttataaccca gatacgggca ccagaaatgg
       61 tgcctttttt attgcagaaa agcgagaggt aattatgcgt aaagtttgtg cagccatttt
      121 gtccgcagcc atctgtctgg ctgtatccgg tgcgcctgca tgggcgtctg aacatcagtc
      181 cacgctgagc gccgggtatc ttcagcccca tactgatatg ccaggcagcg atgacctgaa
      241 gggcattaac gtgaaatacc gttatgagtt tacggacacg ttggggctgg tgacgtcatt
      301 cagctatgcc aacgctgaag atgagcaaaa aacgcattac agcgataccc gctggcatga
      361 ggattccgtg cgtaaccgct ggttcagcgt gatggcgggg ccgtctgtgc gcgtgaatga
      421 atggttcagc gcgtatgcga tggcgggcgt ggcttacagc cgtgtgtcga ccttctccgg
      481 ggattatctc cgcgtaactg acaacaaggg gaaaacgcac gatgtgctga ccgggagtga
      541 tgacaaccgc cacagcaaca cgtctctggc gtggggggct ggcgtgcagt ttaacccgac
      601 cgaatccgtg gccattgacc ttgcttatga aggttccggc agtggcgact ggcgcactga
      661 cggtttcatc gtgggtgtcg gttataagtt ctgattagcc aggtaacaca gtgttatgac
      721 agcccgctgg ttcaggcggg ctttttgtgg ggtgaatatg gcagtaaaga tttcaggtgt
      781 actgaaagat ggggcgggta aacctgtcgt aaattgtgcg attgaactgc gggccagaag
      841 aaccagtccg accgttgtgg cacacgttgt tgccacttgc gtgacggaca ataacggtgc
      901 ttatgtgatt gaggctgagc cggggtatta cgaggttgcg cttcactgta acggctggca
      961 gccaacccgt gtcggggata ttgatgtggc accgactgat gcaccgggga cactgaacgc
     1021 gtttctgaat gcaccaaagg atggtgattt acgtccggag gtgatgaagc gctttgagga
     1081 aatggtggtg caggcgcagc agagcgccgg ggccgcagcc ggaaacgcac agcagacggc
     1141 gcaggatgtg gcggcagccg caacggcccg tgatgatgca caacgttttg cggagaatgc
     1201 cagacaggat gcaaccgtca cagctgagga cagaaaggcc actgcggaag atgtgacaag
     1261 cacaggagca aatgcagccg cagccggaca gagcgcacag gatgccgcag gttatgcccg
     1321 cgcagcagaa caggccaaaa atgacattga tgctgcgctg accggcactc tgaaaatggc
     1381 taaccatctg tcagaaatcg cagcagcagg cgaaaaggca caacagaagt cccgggataa
     1441 tctggggctg aaaag
//
LOCUS       DAJTOR010000326         1389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTOR010000326 DAJTOR010000000
VERSION     DAJTOR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.335"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>1389
                     /locus_tag="OO249_004216"
     CDS             <1..>1389
                     /locus_tag="OO249_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5638276.1"
                     /translation="QLALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPG
                     YFQITAVPKLAVYDPTVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGH
                     DYYFYVRSVNTVGKSAFVEAVGRASDDAEGYLSFYKGLINNTHLGKELWTQIDNGQLA
                     PDLTEIRTSITNVSNEITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMK
                     DGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVF
                     LKRLTAPTITSGGNPPAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGK
                     LSANQIEGDLVKTVGKPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAK
                     HERKNNNIYSSCSLIVKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWL
                     VNGWYPTASISD"
BASE COUNT          358 a          316 c          400 g          315 t
ORIGIN      
        1 gcagctggcg ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca
       61 gggcgatccg gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga
      121 actgataccg gggtattttc agataacggc ggtcccgaaa ctggctgtat acgacccgac
      181 ggtgcagttt gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac
      241 cagcgcccgc tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc
      301 gggccatgat tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt
      361 ggaggccgtc ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt
      421 gatcaataac acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc
      481 gccggacctg actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac
      541 cgtcaataaa aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt
      601 tgatacaaat aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg
      661 acgcctttat attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag
      721 tcaggtgctg ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa
      781 gccgatgttt gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct
      841 gacggctccg accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg
      901 acggctgacg gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct
      961 caacaacgtc acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat
     1021 tgaaggcgat ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga
     1081 gaggtggcca tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca
     1141 aattgttatt cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat
     1201 ttattcgtca tgcagcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac
     1261 cctggataat acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat
     1321 gacgctggag ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat
     1381 cagcgattt
//
LOCUS       DAJTOR010000327         1386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTOR010000327 DAJTOR010000000
VERSION     DAJTOR010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.336"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..112)
                     /gene="ariR"
                     /locus_tag="OO249_004217"
     CDS             complement(<1..112)
                     /gene="ariR"
                     /locus_tag="OO249_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCR5638277.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQA"
     gene            complement(141..413)
                     /locus_tag="OO249_004218"
     CDS             complement(141..413)
                     /locus_tag="OO249_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCR5638278.1"
                     /translation="MKTSDNERIKYEITGQVVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(456..692)
                     /locus_tag="OO249_004219"
     CDS             complement(456..692)
                     /locus_tag="OO249_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCR5638279.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANWPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            1006..>1386
                     /gene="bluF"
                     /locus_tag="OO249_004220"
     CDS             1006..>1386
                     /gene="bluF"
                     /locus_tag="OO249_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCR5638280.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQ"
BASE COUNT          407 a          288 c          248 g          443 t
ORIGIN      
        1 cagcctgccc taacactgct gactcttctt ctaacaaatt acccgaactg cgaaagtaac
       61 ttgctaacgc tttatcagtt attgcattat gaattgtagt atcttcaagc atggtaacct
      121 ctcatcttac ttatgaaatt ttaatgtatt ctgtttattt tcttaccatt gaacatactt
      181 ttattgtcag ctgtcgctgt tcttcggttt ggccctcctg aatcactatt gctaaaatca
      241 cgaattatgc tgtcaattaa atcgcgtcgg aaagcatctt cctcagccga tttcattacg
      301 agtaattgct taataagagt ctgcaatgaa aaatttatct tcatgcgcag tatctggagc
      361 accacctggc cagtaatttc atattttata cgttcattat cagatgtctt cataacgact
      421 tcatagctaa cctgttaaat aatcaggctg ttgcattatt caaaaagcaa cccaattagt
      481 gcattataat gtttctgttc ctcttcccca gtcgcgtgtt ccagtcggca aagaagtttg
      541 gcacaaagcg actttcgact cagattacgt ccatctttca aaatctcagt cacaatttcc
      601 ccgagagttt cctgctgagt aggccagtta gcttttgcaa agtaacgagt gattgctccc
      661 gcagaatcta aagtcactga attttgatgc atgctacgcc tctgttaaaa atgttaatca
      721 atgttaagtg aggtaacaat agcaaacttg tacgaaatat gtgtacagaa tgataaatta
      781 attttttgca attagtgcca atacatataa aacaatgtgt tatgataaaa catattgcat
      841 catttaactg acaatctata cgagattttt cgccaactag tgttcaactt tttccatttt
      901 ttatcaatgt aaagaaacgg ttatgttttg gtattaaaag aaagacacaa gaagatatgt
      961 ctgttaccgt cttactctcg cctcacccat taccctggat tgttaatgct taccaccctt
     1021 atttatcgta gccatatacg tgacgacgaa cctgtcaaaa aaatcgaaga aatggtttcg
     1081 atagcaaatc gcaggaacat gcagtctgac gtaacaggga tcttactgtt taatggttct
     1141 cattttttcc agcttctgga aggtccggaa gaacaggtta aaatgatata tcgggctata
     1201 tgccaggatc cacggcacta taatattgtt gagctgctgt gcgattacgc gcctgctcgc
     1261 cgttttggca aagcgggaat ggaattattt gatttgcgcc tgcacgagcg agatgacgtt
     1321 ttacaggccg tattcgacaa aggcacatca aaatttcagc taacttatga tgacagagcg
     1381 ctacaa
//
LOCUS       DAJTOR010000328         1327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTOR010000328 DAJTOR010000000
VERSION     DAJTOR010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.337"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..571
                     /locus_tag="OO249_004221"
     CDS             <1..571
                     /locus_tag="OO249_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5638281.1"
                     /translation="CLVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGT
                     GWHPYFPLSPQTRIQAQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGF
                     AGWNGQARIEQPQEGYAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDD
                     HHRPEGGDLIALAPGESTTSEMSLRVEWL"
     gene            complement(619..1041)
                     /locus_tag="OO249_004222"
     CDS             complement(619..1041)
                     /locus_tag="OO249_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5638282.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIILGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(1138..>1327)
                     /locus_tag="OO249_004223"
     CDS             complement(1138..>1327)
                     /locus_tag="OO249_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenoxybenzoate dioxygenase"
                     /protein_id="HCR5638283.1"
                     /translation="WLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSG
                     KTFDAKLLIDENIALKSL"
BASE COUNT          335 a          349 c          337 g          306 t
ORIGIN      
        1 gtgtctggtg tatgaacatc gcagcggtgt ctatcactat cgggtaagtc aggcgtttca
       61 tttaacggcg gatacgctga cggtgacgct ctctgtcacc aatcaagggg cagagacgct
      121 gccatttggt accggctggc atccttattt tccgttgtca ccacaaacgc ggattcaggc
      181 gcaggcgagc ggttactggc tggagcggga gcagtggctg gcgggtgagt tttgcgagca
      241 gctaccgcag gaactggatt ttaaccagct cgcgccgttg ccgcgccagt gggtgaacaa
      301 tggttttgcc ggatggaatg gtcaggcccg catcgagcag ccgcaggaag gttatgccat
      361 catcatggaa acgacgccac ctgcaccgtg ttatttcatc tttgtttccg accctgcgtt
      421 tgataaagga tatgcgtttg atttcttctg tctggaaccg atgagccatg cgccggatga
      481 tcatcatcgc ccggaagggg gtgatctcat tgcgctagca ccaggggaat caacaacttc
      541 agagatgtcg ttgcgggtag agtggctgta atttattaaa tgccggataa ggcgttcacg
      601 ccgcatccgg catctttcct aacgccgatc gagagaaatt gcccccggcc cggtaattgc
      661 caatagcaag aacgcgccag cgatactgac attcttccag aaattaatca tatttggccc
      721 aaccgcatcg ccggtcatat cccagtaatg gtgaccaatc accgccgtac ccagcgtgta
      781 gaaaataaac agcaccgcca gcggacgggt gaaaaagcca agcacgatta atatcgcggc
      841 gggcacttcc ataactaccg caataatcgc tgccagcatc ggcattggcg cgcccaacga
      901 ggccatatat tggaccgtac cgtcaaagcc catcattttg ggaaaaccca aaataatgaa
      961 aattaagacc acggcgatac gggcaattaa taacaaaacg gggcgggcag ctccaaaatc
     1021 aaaataacgt agtgtgttca taaccaatcc ctttgcaaga taatgtgatt taaacgtaat
     1081 acacactctt gcacctcgcc acgccccagg cgttgagata ttcgctaatt atcctggtta
     1141 cagtgattta agcgcgatgt tctcatctat cagcagtttc gcatcaaacg ttttgccgct
     1201 ctggatccat ttgcgaattg ggcgaatctc acgcccctga ttcagcgtga ccgcaccgat
     1261 aagcacgccg ttttgcagat taaaccaaat cgccttctga gtttccgggt tgccacgaca
     1321 aagccag
//
LOCUS       DAJTOR010000329         1322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTOR010000329 DAJTOR010000000
VERSION     DAJTOR010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.338"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(32..>1322)
                     /locus_tag="OO249_004224"
     CDS             complement(32..>1322)
                     /locus_tag="OO249_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCR5638284.1"
                     /translation="NGDIAHDVRNQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFS
                     ALLGFTGWRMSKRPQAAEAEEKSLETLTRTITETSEQQVSWETIPLIEPISLSLGYKL
                     VALVDKAQGNPLTQRIRGVRQVISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADE
                     ADIPADKLMAQPSSETYGEIDGVLGNDPAYGMPVTWIQPAQKAKVLNMGYQVIDSASV
                     IATHVNKIVRSYIPDLFNYDDITQLHNRLSSMAPRLAEDLSAVLNYSQLLKVYRALLT
                     EGVSLRDIVTIATVLVASSAVTKDHILLAADVRLALRRSITHPFVRKQELTVYTLNNE
                     LENLLTNLVNQAQQGGKVMLDSVPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQ
                     LRPLLARYARLFAPGLHVLSYNEVPDELELKIMGALM"
BASE COUNT          271 a          398 c          354 g          299 t
ORIGIN      
        1 catccggcag ttgtgcacca atgctggata cttacatcaa cgcccccata atcttcaact
       61 ccagctcgtc cggcacttcg ttatacgaca gcacatgcag ccccggcgca aacaaccttg
      121 cataacgcgc cagcaaaggg cgcagctgcg gtggcaccag cagcaccggg tctttccccg
      181 ccgctttcat ctgctctttc acctgtggca tcgtactctg gaactggttg agcatattcg
      241 ggtccactgg cacgctgtcg agcatcactt taccgccctg ttgcgcctga ttcactaggt
      301 tagtcagcag attttccagc tcattattca gggtatacac cgtcagctcc tgcttgcgaa
      361 cgaacggatg ggtaatgctg cgccgcagcg ccaggcgcac atcggctgcc agcagaatat
      421 gatctttggt caccgcgcta ctggcgacca gcacggtggc gatggtgacg atatcgcgca
      481 gggaaacgcc ttcggtcagc agcgcccggt acactttcag caactggctg taattgagca
      541 ccgcgcttaa atcctccgcc aggcgcggtg ccatcgacga caaacggtta tgcaactgcg
      601 taatatcgtc atagttaaat aaatcaggaa tatagctgcg cacaatctta tttacatggg
      661 tagcaatcac gctggcgctg tcgatcacct gataccccat attcagcacc ttcgctttct
      721 gcgcaggctg gatccaggtc accggcatcc cgtacgccgg gtcgttcccc agcacgccgt
      781 ctatctcgcc gtaggtttcg ctggagggct gggccatcag tttatccgcc ggaatatccg
      841 cttcatcagc cttaatgccg ttgatgaaaa tagcgtactg gctgggctta aggcggaagt
      901 tttcccgaat gcggatctcc ggcagcagca cgccgttgcc gtcggatatc acctgtcgca
      961 cgccgcgaat acgctgggtg agcgggttgc cctgggcttt atcgaccagc gccaccagct
     1021 tgtagccgag gcttaaactg ataggctcga taagcggaat ggtttcccaa ctgacctgtt
     1081 gctcgctggt ttcggtgatg gtgcgggtca gcgtttcgag acttttctct tccgcttccg
     1141 ccgcctgcgg gcgtttgctc atccgccagc cggtaaagcc aagcagggcg ctgaacagca
     1201 aaaacggcag atgtggcatt cccggcacta ccgccagtac gaacataatt ccggtagcgg
     1261 tgtagagcac cgatgggctt gccagcaact ggttacgcac gtcatgggcg atatcgccgt
     1321 tg
//
LOCUS       DAJTOR010000330         1322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTOR010000330 DAJTOR010000000
VERSION     DAJTOR010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.339"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            83..449
                     /locus_tag="OO249_004225"
                     /pseudo
     CDS             83..449
                     /locus_tag="OO249_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            377..850
                     /locus_tag="OO249_004226"
                     /pseudo
     CDS             377..850
                     /locus_tag="OO249_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          322 a          334 c          401 g          265 t
ORIGIN      
        1 cttgccggtg ttctccgggg taaaaaggtc cacactaccg tcagccggaa agccgttgcc
       61 gcaggcgacc gcgtaaaccg tcatcaaggc aatgtgccgc gtactccggg tggcccgcag
      121 cggctggtat acgtggtgtc agcggcggac aaggataaac acacgtcagc agttccgtca
      181 gcactgcgac agcgttgtcc gcaaggcttt tacccagtcc agcgttacgg tgccccacgc
      241 ctgacggatg aactgtgtgc tctggttacc actttaacgt aaaaaccgtg gcagccagtc
      301 tgcgccgtca ggaactgagc gcaaaagcct cccagaagtt cagcccgatc agttaccgtg
      361 cacacggcct gcctgtatca gaaaatctgc tgacgcagga tttttacgcc agtggcccga
      421 accagaagtg ggcgggagac atcacgtact attacagctc accgactgcg gggaaacacg
      481 gggcgccggg ttattgaagg tgacctgtcc agctacttcg acacagtaca tcaccggctg
      541 ctgatgaaag ccgtctgccg caggatcagt gatgcacgtt ttatgagact gctgtggaaa
      601 acgccgtgtt gatgttggtc tcttccgggc ggtcagtgaa agtatgccac agggcggtgt
      661 gatctcgccg ttgctgtcga acatcatact gaatgagttc gaccagtatc tgaataaacg
      721 ctacctgagt ggaaaagcca gaaaggatcg ctggtactgg aatcacagta tccagcgagg
      781 ccgaagtact gctgtcaaag aaaactggca atggaaacct gccgttgctt actgttgcta
      841 tgccgatgac tgtgtgccac gaaggcgcgt cctggggacg tgagtccctg tgcagccatg
      901 cggcacaaga gatgagggaa ggcccctcgg tgtcggcgtg caggcgcagg caccaaacca
      961 tccactgctg ctgcatttaa gagatgtggt ggtgagcgag atgggaaaag gtcggacgtg
     1021 agccgatcag gtatggttcg aagagacgaa cgaaagtgaa ccgctgatga tgtgtcgaaa
     1081 gcatatagat gatgtcaaaa caatggtgga gtcgttcctg tgggatatgt ttactgcctg
     1141 cgcggcatcc ggcataaaag cggcgtgaag tcgattcagg ctcttgtggg gaacgtggac
     1201 ctgtcacccc gatgtgaagg gagaagcgca agtggaagcc ccatgagcgc cagagtacca
     1261 atgcggggta cagggacgga gcaccaagcg attgcgcagt aaacatgctt gacaacttaa
     1321 tt
//
LOCUS       DAJTOR010000331         1293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTOR010000331 DAJTOR010000000
VERSION     DAJTOR010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.341"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            231..>1293
                     /locus_tag="OO249_004227"
     CDS             231..>1293
                     /locus_tag="OO249_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5638285.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPGTYDRPDPRIKQLARRST
                     VTPGGAACKYNDIILADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALE
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPW
                     ICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIR
                     GIGYYGSKWRDSSATWTSQD"
BASE COUNT          312 a          313 c          385 g          283 t
ORIGIN      
        1 ttgtgggcaa aaaaagcccg cgctgggaga cgcgggcggc aaggaataaa caataaaacg
       61 tgaagtaata tttcagctgg cgaataatac cccatagtag tcactctgcg caactgcgcg
      121 gtctttttcg aattgcgggc tgtcgtctct cttctgccat tgtcctgtaa cttccggact
      181 tcagcccgct cctcatttta ctcacaatat tatcccggcc gggaggattc atggctttta
      241 aacactatga tgttgtcagg gcggcgtcgc cgtcagatct tgcggaaaag ctgacacata
      301 aactgaaaga gggctggcag ccgtttggta gtccggtggc cataacccct tatactctga
      361 tgcaggcgat tgcagcagaa ggtgcagtaa tcagcgccac cagcgacccg gagtattact
      421 ttgttgtggt tctggcaggg cagtcaaacg gcatgtcgta tggtgaaggc cttccgctgc
      481 cggggacata tgaccgtccg gacccgcgta ttaagcaact ggcgcgtcgc agtacggtga
      541 caccgggcgg tgcagcatgc aaatataacg acatcattct ggcggaccat tgtctgcatg
      601 atgtgcagga catgagccgt cttaaccatc cgaaagcgga cctgtcaaag gggcagtacg
      661 gaaccgtggg gcaggggctg catatcgcca aaaaactgct gccgtttata ccggcgaatg
      721 cgggcattct gctggttccg tgctgtcgtg gtggttcagc gttcaccacc ggagctgatg
      781 gcacatacag tgacgcgagt ggtgcctcgg agaattcaac ccgctggggt gtggacaagc
      841 cgctgtataa ggaccttatc ggtcgaacaa aagcggcact ggagaagaac ccgaaaaatg
      901 tgctgtttgc cgtggtgtgg atgcaggggg aatttgattt tggcggtacg ccggcaaatc
      961 atgccgcaca gtttggtgcg ctggttgata aattccgtgc agacctggcg gatatggcag
     1021 gccagtgcgt cggtggctct gctggcggtg ttccctggat atgcggggac acgacgtatt
     1081 tctggaagca gaagaacgaa tccacgtacc agacggtgta cggcagctat aaaaataaaa
     1141 cggaaaagaa tatccatttc gtaccgttca tgaccgatga gaacggggtg aatgtgccga
     1201 cgaacaaacc ggaagaagac ccggacattc ggggtatcgg ttattacggt tcgaaatggc
     1261 gtgacagctc agccacctgg acgtcacagg aca
//
LOCUS       DAJTOR010000332         1292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTOR010000332 DAJTOR010000000
VERSION     DAJTOR010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.342"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..909
                     /gene="pocR"
                     /locus_tag="OO249_004228"
     CDS             <1..909
                     /gene="pocR"
                     /locus_tag="OO249_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HCR5638286.1"
                     /translation="SAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFTPF
                     CQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGFVL
                     CGQVRLRNDYVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVENC
                     LKKQLNFVVIKENKSQSDSVRQTRAPSPHDNKMKKALRYIDAHLSDDLRLEDVASHVY
                     LSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYFCK
                     VFRQTYQVTPQVYRQQVSENAAMETV"
     regulatory      986..1161
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          348 a          277 c          321 g          346 t
ORIGIN      
        1 tctgcggcga cacttaattc agaactcatt aataaaatcg cgcaggattt tgcgcaggcg
       61 accagccttg cggttgtcgt ggtcaatatt cacggcgatg aaatttccga gctgtttaac
      121 ttcactccat tttgtcagat gatgcggcaa caccctcagc taagcactcg atgtcgcatg
      181 agcgatcgtt gtgggggcct ggaagcctct aaatccgatc agccttgtat ttatcgctgt
      241 catgccggat taaccgattt ctctattcca ctggtaatcg ccgggcattt agtcggtttt
      301 gtattgtgcg ggcaagttcg tctgcgtaat gattatgtcg atttggtcga tattctgaat
      361 gtcgatgatt gctggcaggc agatcccgaa ttacttaatg aatttcgcaa agttcctgag
      421 atggattact caagagttat ggcctctgct gatttgttga agttgattgt agaaaattgc
      481 ctcaaaaaac aactcaattt tgtggttatt aaagagaaca aatctcagtc agattcggtg
      541 cgccagactc gtgcgcctag tccccacgac aataaaatga aaaaagcttt acgctatatt
      601 gatgcgcatt tatcagacga tttgcgtctg gaagatgtcg cctcgcacgt ttatctaagc
      661 ccttattatt tcagtaagtt attcaagaaa taccatggaa tcgggtttaa tgcgtgggta
      721 aaccaacaac ggatggccag cgcgcgtgag ttactgtgcc atagtgactg gagcatcgcc
      781 agcattgcgc gtaatttagg tttttcgcaa accagttatt tttgcaaagt tttccgtcag
      841 acttatcagg ttacgccgca ggtgtatcga cagcaagtga gtgaaaatgc ggcgatggaa
      901 acggtataaa aaaagggaag gtttaaataa acgctggaat gaataattaa cgttatttac
      961 gtgttatggg aaaagtaaaa tcagctcata acatacgctg acaggtgtgg cacatttgtg
     1021 cctgggaaga gagtgaaaat ccctcgcagc ccccgctgct gtaatgctga caaacccgaa
     1081 aatgccactg atcgcgagat tgggaaggtc gggagaggat gacgctaagc cagaagacca
     1141 gccagtcagt caataccaac aacttcggtg ggaagtgggt tgcccggaag cgtccggtag
     1201 agtacaggcg cgtctttata cccctaccac cctgaaagga tcagggtatg gcggcatcat
     1261 ttagttcttt catcgcagtg ccgacaggca cc
//
LOCUS       DAJTOR010000333         1290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTOR010000333 DAJTOR010000000
VERSION     DAJTOR010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.343"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..349)
                     /locus_tag="OO249_004229"
                     /pseudo
     CDS             complement(1..349)
                     /locus_tag="OO249_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            535..>1290
                     /locus_tag="OO249_004230"
     CDS             535..>1290
                     /locus_tag="OO249_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5638287.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVW"
BASE COUNT          364 a          238 c          279 g          409 t
ORIGIN      
        1 cgccctgagt ttcccgatta tcgaaaactg ttcagggagt ctgacatcaa gagcgcggta
       61 gcctttttaa tatttcattt tccatttcaa tacgttgtag ctttttcctg agctcacgga
      121 tttcaatttg ttccggggta atgggggagg cttttggtgt ttttccctgc cgctcatcac
      181 gtaattgttt cacccatcgc gtcattgtgg aaaggccgac atccatagcg ctggctgcat
      241 ctgccacggt gtatttctgg tcaacgacca gttgagcgga ttcgcgttta aactctgcgc
      301 tgaaatttct tttttcatta tggcacctgt gttgttctga ggtgagcata tcacctctgt
      361 tcaggtagct aaatttagtg tgccacctca aggttcttag tgagccgttt ttaaaagata
      421 tgataatata ttattgttta taaattgttg atgtttgatt tagtagtata taaagttcac
      481 cacctttatt aggctttcct tttatctcta cttactgggt gccagaaact ttaaatttta
      541 aacggagggg gcattttgtt taaagcaaat catgttccgg tattaatgta tcatcatgtc
      601 agccattgcc caggactggt tacactttct cccgagacat tccggaaaca aatgaaatgg
      661 ttggctgaaa acaactggaa aacactgtca tcagatgaac tggagttttt ttatcggggc
      721 ggaaaacttc cccgtaaaag cgttatgttg acatttgatg atggatacct tgacaactgg
      781 tttcaggtat atcctttatt aaacgagttt aatctcaagg cacatatttt tttaattacc
      841 agttttattg gaaatggtcc ggttcgtcac tctcctggaa aggaatattc tcatcgcgat
      901 tgcgaacatc aaatagcaac cgggaatgct gataatgtta tgcttcgctg gtctgaagtg
      961 aacgaaatgt tgcaatctgg tctggtcgaa tttcatgttc atacacatac tcatacacgt
     1021 tgggataaaa agttcacttc acgagaagaa cagtgtaaac atcttcggca ggacttattg
     1081 agcgggagag aatatttaaa aaaaatgaca ggaaaatgca gtaaacattt gtgttggccg
     1141 gaaggttatt ataacaagga ttatattcag atcgctgaag aacttggatt ccattattta
     1201 tatacaacag aaagaagaat gaatgctcct gctaaaggaa ctacacggat aggaagaata
     1261 agcaccaagg aacgggaaaa ttgcgtatgg
//
LOCUS       DAJTOR010000334         1290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTOR010000334 DAJTOR010000000
VERSION     DAJTOR010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.344"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO249_004231"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OO249_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5638288.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
     gene            1117..>1290
                     /locus_tag="OO249_004232"
     CDS             1117..>1290
                     /locus_tag="OO249_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5638289.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERY"
BASE COUNT          397 a          223 c          239 g          431 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcagaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaaaga gagctaaata gctgcgcgga atagtaggct gtgtccctta atcatctgag
     1081 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     1141 gaggcatgga ccatcatcaa gcccctgtta cctcctgaac ccgccacacc acgggccgga
     1201 cgcccatggg ctgagcaccg taaaatcatc aatggcatgt tctgggtgtt atgttccggt
     1261 gcaccatggc gcgatttacc cgaacgatat
//
LOCUS       DAJTOR010000335         1279 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTOR010000335 DAJTOR010000000
VERSION     DAJTOR010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.345"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..702)
                     /gene="corA"
                     /locus_tag="OO249_004233"
     CDS             complement(<1..702)
                     /gene="corA"
                     /locus_tag="OO249_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5638290.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLF"
     gene            1072..>1279
                     /locus_tag="OO249_004234"
     CDS             1072..>1279
                     /locus_tag="OO249_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638291.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAW"
BASE COUNT          328 a          336 c          313 g          302 t
ORIGIN      
        1 gaacagagat tcgttatgcg gcagcaggga ttcgatatct cgcaggattt cacgcgcctg
       61 ctccagttgt ccacccggta agcgtgcttt acgtaccagg aagttgagcg cgcgctgggt
      121 atccatcaga cacaggcgta ctttccagcc gatatcttcc agttccgcca gcgtggagag
      181 cgcctcatcg tactcatcgc cctgatgccc ttccataatc acccggctca actgctccag
      241 gtcgctatag atattttcaa tttcatctgc caactgttcg attttggttt cgaacagatc
      301 cagcagcagc tcgtaggcgt taccgtctac catcgactgg ctacgggcac gcatacgata
      361 cagacgaaaa gcgggcagtt cacgctcacg cagagtaaac agacgaccat cacggatggt
      421 aaatgccaca gtggagttac cggcgtgatc ttccgcatct tcaaagaaga agaaggagtg
      481 aatatgcagg ccgtcgtcgt cttcaaagaa acgtgccgat gcttcgatgt cttccagttc
      541 cgggcgggtt gccaggctct ggccaagttc agattgtaca cgcagtcgct cgtcgtcgtc
      601 cggttcgaca agatcaatcc atactgcatt tacaaggggt tgtgactctt cgacttccag
      661 ccgggtcagt cggttatttt ccagttgaaa tgcgctcagc atgaccggga ctcccaatgc
      721 ttaaaatatc ggacagttcg gtgggcacac agaaacaaat tgggtttcag accattaaac
      781 agcctgactc agcgcgacgg gaaaaattga ggtcgctgac aaccgctaag gctatcagca
      841 aaaagggata gccttaggag ttgatcctgg atgacaggat agtgagccag tatctactgg
      901 gtgtgtccaa ggcgaatgtc ctcttagaat gatcgtgcgc gcatgttacg ccagcaaaat
      961 tttgccgtca acacgcaaca gaacaccaca gaacaatagt tgaacttcac gctttttggt
     1021 actcctcaag gtcgctaaca ggataaaaaa atcccacatc caggacctat aatggcgcat
     1081 cggctactca ttggtaaagg aatgatcacc ttgaatctca aacggatttt tctcaccctc
     1141 accttacttc ccttgttttc tgttgccgct gatgattgcg cactctcaga tccgacgctg
     1201 accgtacagg cgtataccgt taatcctcaa acagagcggg tgaaaatgta ctggcaaaaa
     1261 gccaatggcg aagcgtggg
//
LOCUS       DAJTOR010000336         1269 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTOR010000336 DAJTOR010000000
VERSION     DAJTOR010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.346"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..680
                     /locus_tag="OO249_004235"
     CDS             <1..680
                     /locus_tag="OO249_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCR5638292.1"
                     /translation="TQHDHYDRLSVRLSVIISRLLAGESLELKALADEFGVSERTLQR
                     DFQQRLIHLDLEHSSGSYRLTCRTGREHSPNTFAFIRNSGISGIIPAQNRRLIRLITS
                     NSGSSPCLIGHGGLPSPVTQTACFLELAEAIRDYRAVTLLVNGKSYEAVAPRRLIFQS
                     KNWFLVATRTRNLQVFRMEDVNAVTVLDTSFRRKPELDALTASEEFISALPHFRFISD
                     VIQTFRE"
     gene            719..>1269
                     /locus_tag="OO249_004236"
     CDS             719..>1269
                     /locus_tag="OO249_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR5638293.1"
                     /translation="MNDPVKGEGRMITDASGVTRREGGILGMWSNIFPERNGSNEQKG
                     DSKPHIPDIRDILDIFPQPLREKLLNKISEAIDYEPVIGVMGKTGAGKSSVCNALFKG
                     EVCAVSDVEACTREVQELRIRFGKHSLKIIDIPSVGENARRDKEYEYLYRNLLPSLDL
                     ILWVIKGDDRAFSADEHFYNNVLL"
BASE COUNT          336 a          294 c          316 g          323 t
ORIGIN      
        1 aaacacagca tgatcattat gaccgcctgt ccgtcaggct atcggtcatt atcagccgct
       61 tactggctgg cgaatctctg gagcttaaag cgttagctga tgaatttggt gtctcagaac
      121 gcaccctaca gagggatttt cagcaacgcc tgatccatct ggatctcgag cattccagcg
      181 gaagctaccg acttacctgc cgaacgggcc gggagcactc acccaacaca tttgcgttca
      241 tccgtaattc cgggatttca gggattattc cggctcagaa tcgcaggtta attcgactga
      301 ttaccagtaa ctccggaagc tcaccgtgcc ttatagggca tggagggctg ccatctccag
      361 ttacacaaac cgcctgtttt ctggaactgg cggaagctat ccgtgactac cgcgccgtta
      421 cattattagt taacggtaaa agctacgaag cagtagctcc ccgccgtctg atatttcagt
      481 ccaaaaactg gtttcttgtt gctacccgca ccagaaattt acaggtgttt cggatggaag
      541 atgttaacgc cgtaaccgtt ctggatacct cttttcgcag aaagcctgaa cttgatgctc
      601 tgactgccag tgaagagttt atttcagccc tccctcactt tcgtttcata agtgacgtaa
      661 ttcagacctt cagagagtaa cagacaacgt tcatttcaaa ttaaaaagga gacctcgtat
      721 gaatgacccg gtaaagggcg aaggccggat gattactgat gccagtggcg tcaccagaag
      781 ggaaggcggg attttgggaa tgtggagcaa tatatttcca gaacgtaatg gtagcaacga
      841 gcaaaaaggt gatagcaagc cgcacatacc cgacatcagg gatattcttg atattttccc
      901 tcagccactg cgggaaaagc tgttgaacaa aatcagtgaa gccatcgact atgaacccgt
      961 gattggtgtc atgggaaaaa ctggcgcggg gaaaagcagc gtatgtaatg cgctgtttaa
     1021 aggggaggtt tgtgcagtta gcgacgttga ggcatgtact cgcgaagttc aggaattgcg
     1081 tatacgcttc ggcaaacatt cgctaaagat cattgatata cctagtgtcg gtgagaatgc
     1141 ccggcgggac aaggagtatg aatatttata ccgtaatttg cttccctccc tggatcttat
     1201 cctgtgggta ataaaaggag acgatcgggc cttttctgcg gatgagcatt tctacaataa
     1261 tgttctgct
//
LOCUS       DAJTOR010000337         1266 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTOR010000337 DAJTOR010000000
VERSION     DAJTOR010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.347"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(372..638)
                     /locus_tag="OO249_004237"
     CDS             complement(372..638)
                     /locus_tag="OO249_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638294.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPATNRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
     gene            complement(820..1218)
                     /locus_tag="OO249_004238"
                     /pseudo
     CDS             complement(820..1218)
                     /locus_tag="OO249_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          331 a          259 c          260 g          416 t
ORIGIN      
        1 acatgcttga caacttaata ggggcacccc ccaaagccgt ctatgggaag gagtctgctg
       61 ttattccaga gaatggtaat tcagtcatat atttctccat ctatgacttg tcaataccat
      121 cctgatattt gtgggtcagc aatatattct actaaaaggt atttaaggtc attgcgtccc
      181 tgcactcgaa taatggtgaa gaaagcatga tgaatatatc acgataacgt tcagtatgta
      241 tcgaggatag atggagttac tcctctctga aatagaatta ctgctctact ttttctgttt
      301 ccagaaatta ttagtaacaa accctgatat attcttttga aaatggagct taaacgagtc
      361 tgaaattaac ttcagatatt agaatgccgt tggctttgtt tttcaacaat tcctacttca
      421 tttatctttg tcaaccattg catttctgta gcagtgaatt tcaacgtttt cgtccggttg
      481 gtagcaggta tttgtaattc gtatatagct gcttttttat tgaaaacagt atatccatct
      541 gtagtaacag tagaagataa taatacagga tttgacactt caaggatgat attttcttgt
      601 ttttgtggaa ggcaagggaa tatagtttgt gtaagcatat tgcttcctta tgttatattt
      661 tagtaaagaa tttatttttt acattatagt cggctaaatg aagtttactg taaaaaaacg
      721 atactttttt cgtaagcgat gagatgtgga gccagaaaaa tggctgcgtt acgtcattga
      781 actgtccttg acccacgata ttcagacagt cgttgtatct taagttgaca ttacccgctg
      841 acgtcgatat tcccttggtg agagataacc caacgcacta tgcggatggt tttcattgta
      901 atgctcgatc gccactgcaa ggttatgcaa tgccgttctt ggtttcggca tgaacgcgat
      961 gcagtcttcc ttcatcgttt tcacgaaccg ttctgctatt ccattactct gagggctact
     1021 gactgccgtc gtgcagggct ccagattcaa ctctctggcg aactgccgtg tttcatgagc
     1081 cgtataagcc gaaccgttat cggccactgg attggccgtt ccggtccttt atcgccatat
     1141 cgctgacatg acatcctgca ccgttgcttt gtcataatct cccgtgcctg ctgcccagtc
     1201 tatggcttca cgatcgcaat atggaacgta acgctgtcat cgggagaatc gtcaatgcgg
     1261 cattcg
//
LOCUS       DAJTOR010000338         1254 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTOR010000338 DAJTOR010000000
VERSION     DAJTOR010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.348"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(47..886)
                     /locus_tag="OO249_004239"
     CDS             complement(47..886)
                     /locus_tag="OO249_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5638295.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESFECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLL
                     SLK"
BASE COUNT          328 a          308 c          286 g          332 t
ORIGIN      
        1 tgtaaaacaa taatcaagca tggggttcat atccatagct ccatttttat tttaaggaaa
       61 gcaactgctc tacggtcatg tttttaattg cgccccggtt tacaagagtc cagccctgtt
      121 tttccagata aaaccggaaa gtctccaggg tacagaccag tgcgccatca ggaacggttt
      181 cggtgaattc gacattgccg aatttgtcga agtgaacaac cagagtgcga ccatcacccg
      241 gaatcatctt gtcagcaggt ggggtgttat tctggcgcag ttcggcctcc atgcggtcga
      301 actcagcaat gtaggcttcc ttgaatgtgg cggctttttt gcccgtgaag cccatcacca
      361 ggaaaacgaa gccgtttttg gtgatttggt aggcgttata agaattgccg cggtgttcga
      421 atttaacccg cgaaaagttg ctggttaaaa atagttcaga acattcgaag gattctattt
      481 tttgaacaac atggtgatgc tgcttgccaa aaaactcagc aatcgaaata gaagtagtga
      541 cggcgcgacc attttcgatg gttacgtcag ggtgagaaag ggtagtagcc ataatgacat
      601 cctcaagtga taagttaatt aactcaccag cagaggtgct aatctcatgg gtggtgagac
      661 gcacagggtt agcactaccg gtcactcgag aacccggcca gccttgcggc tgccccgcac
      721 gcctcaccat aatttgaatg tggctgtgca ttacgcataa aaaaaccgct tcagcgcggt
      781 tatgcgctcg agtaactttc ggggtgctaa tcccggcacc cgttttatga ggtgcaggtg
      841 cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc tctttccttt
      901 catcaccgaa gtgaattttg tgatgcggtg cctagtgcct ccaggtgacg ttaaccagtt
      961 aacaattaca gtcggctttc ccacccaaac caataaggac taacatgact tttaactgtg
     1021 ccgcgtgcgc ttagccgcat tcaccgcatc acaaaattca ctttaaaaag ggcggacatc
     1081 agccagcaat taaaccgatg ccgccaactg gtacttcaca cagcaatgtc gttatttaca
     1141 accggaagcg cactcccacc atttaaattt cacagacaag accgactctt tatgaatacc
     1201 ggaaatgcgc cttcgtgttg tgcccggttt tatttcacca cctccgggct ttgg
//
LOCUS       DAJTOR010000339         1243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTOR010000339 DAJTOR010000000
VERSION     DAJTOR010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.349"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..207
                     /locus_tag="OO249_004240"
     CDS             <1..207
                     /locus_tag="OO249_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCR5638296.1"
                     /translation="DYQRLLEGEVGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWD
                     LQNKAERQNDILVKYRHMSVPPES"
     gene            complement(271..762)
                     /locus_tag="OO249_004241"
     CDS             complement(271..762)
                     /locus_tag="OO249_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5638297.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(864..>1243)
                     /gene="ycdY"
                     /locus_tag="OO249_004242"
     CDS             complement(864..>1243)
                     /gene="ycdY"
                     /locus_tag="OO249_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5638298.1"
                     /translation="VSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPLA
                     DTPADHIGTLLLAASWMEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFWR
                     TMAPLTRDAISAMWDELEEDSEE"
BASE COUNT          324 a          346 c          300 g          273 t
ORIGIN      
        1 gactaccagc gcttgctgga aggggaagtg ggttacacct caaacgaacc cgttatgctg
       61 tgtctgatgt cggctatcga aacaggggtc attttcctga ttaatgatgg tatcgaccgt
      121 ggtctgtggg atttgcaaaa taaagcagaa cggcagaatg acattctggt gaaataccgc
      181 catatgtcgg ttccaccgga atcctgacag ataaaaaagc cggaattatc atccggcatt
      241 ttactgttta agcgaaagcg tgagtgcccc tcacgcttta tttttgattt cctgctcacg
      301 gtgagccgtc tttgcactac gcgccgccag ccacaggccg ctgtttttca ttgcgtaacc
      361 aaataccagc ccaagcgcca gcgaaggtaa caccagtttc cagtcaccct gcccggcaaa
      421 cgtcgcgcat gcgcctataa acgtccccgg aacaaatgaa agcagcagct gtttggcctg
      481 aatacacatc agaaacgcca caataccggt tatgacataa ccgagaattt ccagatgtgg
      541 tgccagtgca ctaccgtaaa taatgaccat cgcccacacc acgccactaa gcagggttgc
      601 agcggagatc gccagccctt tcagcccacc ttgcgggcag gcaaagtagg ccgtacagcc
      661 caggaagccc gcccagctta gtaagcccag ggaaacagcc acccatcccc agataccgga
      721 gagaatgcct gttgtgattg caatagagag aagtatgttc atgccgcaca tgatagcaga
      781 aaacgcggcc atgaatgaga tcgagcacac attttatgca acaacaaatt atctgttgca
      841 gttatgagtg acgatgatca catttactct tcagaatctt cttccagctc gtcccacatt
      901 gcactaatgg catcacgggt tagcggtgcc atggtgcgcc agaaaggcgt ggttgcatgg
      961 gcctccactt tgccgaggaa cgcaccacac cagggtaaca gatactcact gaacagtgtt
     1021 tccagtgctt cgctctcatc ttccgttgac tgatcttcca tccaggaagc cgcaagcagc
     1081 aatgtgccga tgtgatcggc tggcgtatcc gccagtggca tccctcgttc ggaaagaaaa
     1141 gcgcgcactt ccgcttccgt tgcgtcctca acccatgcgc tacgatatgg cggcacggca
     1201 cattcatcgc cgataaacaa cgcattgtaa tcggcagaga ctt
//
LOCUS       DAJTOR010000340         1243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTOR010000340 DAJTOR010000000
VERSION     DAJTOR010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.350"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            219..1166
                     /locus_tag="OO249_004243"
     CDS             219..1166
                     /locus_tag="OO249_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5638299.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
BASE COUNT          340 a          241 c          297 g          365 t
ORIGIN      
        1 ggtttagcac cgtcatgctc gtctactgtt gttctggttg ttggtgatgc ggtggcgctt
       61 gcactctctg aattgaaaaa atttactcgt gctgatttcg gcttatatca tccaggaggt
      121 gcactcggca ttaaagcaaa ttcataattt caaagtataa atacagtgac cggtcacgcc
      181 atcttttggg gtggcgttca ttgaagtgag tgtcagctat gaaaaatgtt ctggtcaccg
      241 tccccagctt ttcagcacgc tgtgtttccg ccagcaagtt gctaagagag aataatttta
      301 atcttattat taagaataat gtggagcatc tgcttaaatc agaatccact gcattacgag
      361 aatctatttg cgcggtcatc gccggaaaag attgttatca ggcggacacg ctatcattgc
      421 tgcctggtgt gaaaataata tcgagattcg ggacggggat agataatatt gatcttcgtg
      481 cagcacaaca atccggtatt gtagtcaata atgcggttgg gattaattca aatgctgtcg
      541 cggaatttat cattggactt atcttcgcga gcatgagaaa tattcccggc agctatcatg
      601 cgatgcaaaa tggctactgg ggtgagtcgc atggctatga attacaaggc aaacggattg
      661 gcatggttgg ctacggcaat ataggtaaaa ccctggcaaa aaggttatcc ggttttgatg
      721 ttgagctttt agcttatgac aaacaacccg attatcaggt tgctgacaaa gttggagttc
      781 agtttgtatc aattgaagat atctttatgc agtcgcatgt catcattgtt cttttgcctt
      841 tttctcctga gctggagaac ttcattagtc ataaatattt gtcgatgatg cgcaatggtg
      901 ctctaatcat caatgccgca aggggaaagt tgctggatga gggcgcttta ctacaggtga
      961 ttgaagaacg aaatgtgttt gcggcgctgg atgtgtttag ctctgaaccg ttggcgcaat
     1021 ttagcccact gctacatgcc aaaaatatca ttacaacgcc gcatattgcg gccgccaccg
     1081 ttgagtcata ccagcaaacc ggtacacacg ttgcccagtc gataattgat tacttcgcag
     1141 gaagggagat aaaaaacgtg ttgtgagttc agcgaacgaa gtttttaaat acaaaaaaac
     1201 cgcaatttgt ggaacacatt gcggtttatc agcattctaa gta
//
LOCUS       DAJTOR010000341         1224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTOR010000341 DAJTOR010000000
VERSION     DAJTOR010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.351"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            130..720
                     /locus_tag="OO249_004244"
     CDS             130..720
                     /locus_tag="OO249_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR5638300.1"
                     /translation="MDSLQDDYTKLLYGLMQPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPVDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            707..>1224
                     /locus_tag="OO249_004245"
     CDS             707..>1224
                     /locus_tag="OO249_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5638301.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRFATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRP"
BASE COUNT          304 a          268 c          359 g          293 t
ORIGIN      
        1 cagtttgtct gctggtgaag tggcccaccg gttaatcgtc ccttcatcgg atattgacct
       61 gggggagacc gagcttccgg tgctcggcga gatcacctgg caggcttatg acccggcaag
      121 gagtaaataa tggattcgtt acaggatgat tatacaaagc tgctgtacgg actgatgcag
      181 ccggggcctg catggtcaga taccgacggt gtacttgacg gtctggcacc atcgcttgtg
      241 cgtgttcacc agcgggctga tgaactggtg attgaaatcg atcccggtca gtccacagag
      301 ctgattgaac gttatgaaga attgtatggt ttacctgatt cctgttcccc tgtcggaacc
      361 cagacattac gccagcgtca gcaacgtctt gaagcaaaag ccaatgtggc tggtggcata
      421 aatgagcagt tcttcctgga tcagcttgag gcgctgggat ataccggcgt aacgatcgaa
      481 cagttccagc acctggatgc aagccccgat ccggaatggg gagatcgctg gcgttatttc
      541 tggcgtgtga cgttgccggt ggatgccggt gctcagtggc agacatgcac ggacgcctgc
      601 aacacaccga tccggacgtg gggggatacg gttgcggagt gcgtgattaa taaattatgt
      661 ccgtcacaca ccgtcgtttt attttcctat ccagatgagg atgaagatgc acaggattga
      721 tacgctgact gcggtaaaag ataagtttgg tccgggtaag aacggattta ctgacggaaa
      781 tcttcgcaca ggacgttttg ctacctggct gaacagtgcc atgtggaatg ccattcagga
      841 ggaaatctgt ggtgtcattg agaaggccgg gatagaactg aataaagaag aacacgacca
      901 gctatataag gctatattat tgctggtggg cggtgcaatt aacgaagagg cattgctgat
      961 aaaaaataac ctttcggatg tggaagacag ggatgaggct gttgaaaacc tcggattaaa
     1021 acccacggtg gataaggcaa aaaatgccgt tcagcgtgat ggcgacacca tgaccggaga
     1081 gctgaaaatc cgtggtgtta atgcgctgag gattttcaac gaagcttttg gcctgatttt
     1141 tcgtcgttcg gaagagtgcc tgcaccttat tcccaccagt gaaggtcagg gggaaaatgg
     1201 cgatattggt cccctgcgcc cgtt
//
LOCUS       DAJTOR010000342         1166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTOR010000342 DAJTOR010000000
VERSION     DAJTOR010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.352"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..314)
                     /locus_tag="OO249_004246"
     CDS             complement(<1..314)
                     /locus_tag="OO249_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638302.1"
                     /translation="MADKAILWALISASTKEERKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IK"
     gene            complement(1022..>1166)
                     /locus_tag="OO249_004247"
     CDS             complement(1022..>1166)
                     /locus_tag="OO249_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5638303.1"
                     /translation="LSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNK
                     ENP"
BASE COUNT          338 a          240 c          196 g          392 t
ORIGIN      
        1 tcttttattt tatttactgt ttcaatgcaa tcagcggcaa gttggagtgg atttagattt
       61 ttcagatatg gtcttatgat ctttccctta cttaataaat agcatgtata ggattcggaa
      121 agtccagcat ctattttgta cctcattata ttagataagg atgtgagaaa ttccttgtta
      181 tcattcgcag ccatatatgc cagtccgagt tcagcctctg cggccttaca ggcaaaatag
      241 cttagtgaac acgcttttcg ttcctcttta gtactggctg atattagtgc ccataatatt
      301 gctttgtctg ccaaatctaa ctctctctga aattgtcttg cagtaactgc actcgcagaa
      361 atagagaaaa aaatacaatt gttaataacc ttgccatatt tttttagtcc gtaactgtta
      421 atgtgctatc tggctctgaa aatattaact catttaattt ttgcatgtca ttagataaac
      481 cagttataca accctcggaa cagaatcgag gtcctaaaga cggaccaaaa gcgtttaaac
      541 aactatgtat tgaaaatcca ttgcgattat aagttcctgt attaactgac ggtactaatc
      601 gtatgccgtc aggttttcta ttgcttgctc ccgggtcatt aacattccaa ctccataggc
      661 cttgaggtat tggccatcta tttataaatg tgtacaacgg ggattatttt tacattgcat
      721 attattacca gaagcgactg gaatactaag cacatcataa aaattatcat cacatggtat
      781 atctgaaaca cacgaaagca tacctaaagt tatgctgtat aaacaggtgg ctaatcccaa
      841 ggggatcgat tccagaaatc gggttcaacg gatactgata aaaattccac ctaccttcca
      901 gcccaatcgg agcctgagtg ttataccgcc cctgctgcgg ttaatgttac cgataatagt
      961 tatagtacag ccctgattca tcatcatact actgctgcgg cagtctgatg aactattgct
     1021 tttacggatt ctccttattc ttccttctat ccattcgctt tagctttttg agctcttgaa
     1081 gtttgctctt tgcatcaata ttaccgttat ttacagcgaa ttccatccaa taaatagctc
     1141 tattcaaatt ataatattct gataat
//
LOCUS       DAJTOR010000343         1157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTOR010000343 DAJTOR010000000
VERSION     DAJTOR010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.353"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..173)
                     /locus_tag="OO249_004248"
     CDS             complement(<1..173)
                     /locus_tag="OO249_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HCR5638304.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWL"
     gene            complement(206..427)
                     /locus_tag="OO249_004249"
     CDS             complement(206..427)
                     /locus_tag="OO249_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5638305.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            553..1062
                     /locus_tag="OO249_004250"
     CDS             553..1062
                     /locus_tag="OO249_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HCR5638306.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALREDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEVTIRDITRLPGGRIFV
                     EGGNRAFEC"
BASE COUNT          340 a          209 c          270 g          338 t
ORIGIN      
        1 agccagattt ctggtgcggt taataaatga ggctgagctg ggttcagctt gttccgcaga
       61 atctgcacat tcatgcctgc acgttctgcc agttgcacca gattgtggcg cagtgcaaat
      121 gcacgacagg cttcatcaaa atgtggatgt ttggaaactt ggtaatcaaa catggtcaat
      181 gcctctgatg tatttcagaa tcgaactaat taaggtttag attgcattct gaaagcgcat
      241 caacggtcat tgctgctatg ttgatcatca ctttttcgcg ttttttatct ttgcgcagac
      301 ggtgacggat aaggcgtcca tcagccaaca tgtcattgat ggtatcgatg gatagccctg
      361 tcagctcgct atagcgttca atagtcacat gaggcgtggt aagagtgatt gaaatgttag
      421 gtctcatgat gcaacattcc tcgtttaatg atgattaatc aggacgaata cggatcgttt
      481 gtattttgtg aacaccataa acatacgatt gcacagtgaa atcgtcaaga taaaagttca
      541 cttggagtga ccatgaattt ggagaaaggc ggacgaggcg ccatagagcg catggtagaa
      601 gcttatggat tcaagactcg acaggcgttg tgcgatcatt taggaatctc taaaagtaca
      661 ctcgccacac gctacatgcg tgactcattc ccagcagaat gggtaatcca gtgcgccctt
      721 gaaacgggca cctcgcttaa ttggctcaca accggacatg gttcaaagca aacttcaggt
      781 aatacaaata ctatggaagt tgctaaatat gtattatctg atggtgcctt gcgtgaagac
      841 ggtttttata tttttgataa gggatttcta ccctctacgt ttaaaaaacc ttttgtcatc
      901 acagataaca attctgaatt tatttgtgat aaagaatttg atgacatacg tgatggtaaa
      961 tgggtaataa gtattgatgg cgaagtaacg atccgtgaca ttactcgttt acccggtgga
     1021 agaatcttcg tcgagggtgg aaacagagcc ttcgaatgtt agatagaaga cattgaaata
     1081 attggtaaaa ttataagttt aacagttaaa tatgttagat aataccggga ggaaactatg
     1141 cttggtaaag tattttt
//
LOCUS       DAJTOR010000344         1156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTOR010000344 DAJTOR010000000
VERSION     DAJTOR010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.354"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..265)
                     /locus_tag="OO249_004251"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="OO249_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OO249_004252"
     CDS             complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OO249_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5638307.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          338 a          285 c          176 g          357 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactctt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tgttttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gagtctaaaa
     1141 agtgatccac ttacca
//
LOCUS       DAJTOR010000345         1133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTOR010000345 DAJTOR010000000
VERSION     DAJTOR010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.355"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(165..788)
                     /locus_tag="OO249_004253"
     CDS             complement(165..788)
                     /locus_tag="OO249_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5638308.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
     gene            complement(739..>1133)
                     /locus_tag="OO249_004254"
     CDS             complement(739..>1133)
                     /locus_tag="OO249_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5638309.1"
                     /translation="RYCYWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIGGL
                     IIAAYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHS
                     LPGSYCALSGGPDAHVSGAMPGLFIRTS"
BASE COUNT          236 a          343 c          274 g          280 t
ORIGIN      
        1 cacagaccgg actgactccg gaaaaacaga ggcccgcccc cgggcctccc cggattcatc
       61 cgtttccctg ttcagcctga cagcacgccc ccgcggccgg atgacagact ccgcttcggt
      121 aagcaaagcg gtcttctgtg attccgccag ttgcggctta ttcattactc aacgtcaaac
      181 gcccgaattg aagccaaatc atccagaccg ctcagctcct ctttcatttc acgctgacgg
      241 cgataaatct catcgttgcg atcgacctgc gcctgcacca ttgctgccgc cagttcttcc
      301 agttccggca tcgacagttt cacctgctga ttatcggcat cgctccacgc catatgtgtt
      361 tgtgctgtga cagattttgc cagcatgact accggggaca ggcggcccag tgagtcggga
      421 ccagcattcc agatacgacc gttccattca aacgtgaacg gcttcgcctc ctgttctgcg
      481 cgccatgctt caatttcctg acgtctggcc tctctggccg cttccagcat ttctggtgtc
      541 acagtgaatg gggctatctc accccatttg ccgctttgca gttcctgcca gatttgctga
      601 cccgtcggag cgacatcatc agcggtggct gtgtagggga ctgcctggtc cctgtcgtca
      661 aaaaaaacgt cacagtctac tgcgccactt tcggtataac ggggattaat gattttttta
      721 atttccacgg tgcattcctc acgatgtgcg aataaaaagc ccgggcattg cgccagagac
      781 atgtgcatcc ggccccccgg acagggcgca atatgacccc ggtaatgaat gctctgaaca
      841 tcccgtaatg aaaaattgtg gggatgctat atacgttccg gtgggagtac tgggcactga
      901 aatccccacc ggtcccagtc gtgagcctct gtatgactgc cccctgacaa gtctgatgac
      961 tttatcaccg tcagcttctc cctggtacgc agcaataatc agcccgccaa tgtcagggtc
     1021 tccccatctg ttgcggacag agctcgccac gattctgtaa ataatatctt ctgtggttat
     1081 atttattttc acccagccag tcagtctgga tatgggccag tagcagtagc ggg
//
LOCUS       DAJTOR010000346         1106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTOR010000346 DAJTOR010000000
VERSION     DAJTOR010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.356"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            81..602
                     /locus_tag="OO249_004255"
     CDS             81..602
                     /locus_tag="OO249_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638310.1"
                     /translation="MDVYANGQHVFRFQNGVAIALKNIQAGNAKKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFYSQRNTDGSITFAVNGQVIPLNYGNFDARYKHRT
                     EGVQDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQ
                     KLINGIWYNVASI"
     gene            605..>1106
                     /locus_tag="OO249_004256"
     CDS             605..>1106
                     /locus_tag="OO249_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5638311.1"
                     /translation="MLHLKNITAGNPKTVEQYQLTKQYDVTWLFSKDGKNWYEELKNF
                     ASDTIKITYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFS
                     VITDKTS"
BASE COUNT          320 a          234 c          285 g          267 t
ORIGIN      
        1 ggcgttcaga acgccctggg gggaattcaa ttgctttcgg ggataacgat acaggtataa
       61 aacaaaacgg cgacggcatt ctggatgttt atgcgaatgg acagcatgta tttcgtttcc
      121 agaatggtgt ggcgatagcg ttaaaaaata ttcaggccgg aaatgctaaa aaattcacgt
      181 tatccagcgc caacaactcc acgaaaaatg cagcgtttta tttgtggggt aatccatcca
      241 ggcctgttgt tgcagagctt ggtgatgatt ccggctggca tttctacagc cagagaaata
      301 ccgatggtag tatcacattc gctgtaaacg gacaggtaat tccgttaaat tacggaaact
      361 tcgatgcccg ctataaacat cgaactgagg gggtacagga tgtgcggtat ggttatgaaa
      421 tgtattacaa ccctggcagc aacaccgttt catggacgtt tcgctcacct tcgggacacg
      481 ggttgtcagg gatatcgata tcggataccg gccgtaactc agcggataac gtcaacggtg
      541 tgtattaccg accgctgcaa aaactgatta atggcatctg gtataacgta gcgagtattt
      601 aacaatgttg catttaaaaa atattacggc aggtaatccg aaaaccgtgg aacaatatca
      661 gctgacaaaa caatatgatg tcacctggct tttttcgaaa gacggaaaaa actggtatga
      721 agagctgaag aatttcgcca gcgacacaat taaaataact tacaccggag acggtcgcgt
      781 ggtgtgggtc ggtaaggatg tgactggcat tgaaccccgt aacgccagtg ttattgaagt
      841 tcctgatatt accgccaacc gccgcattac cgcgccgggt tactggtttt accgcaatga
      901 tgaatttgtc tttgactaca gactcaaagc ggaagatgag cgtgatgccc ttctggctca
      961 ggtcagtgcc cggacagggg aatgggaaga agacctgctg ctggggctaa tcagcgacga
     1021 agaccgggaa aaactgaaag cgtaccgcat ttacgcgaaa tcgctgcagg cgatggattt
     1081 cagtgtcatc actgataaaa cctcat
//
LOCUS       DAJTOR010000347         1103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTOR010000347 DAJTOR010000000
VERSION     DAJTOR010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.357"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(53..1027)
                     /gene="hemB"
                     /locus_tag="OO249_004257"
     CDS             complement(53..1027)
                     /gene="hemB"
                     /locus_tag="OO249_004257"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5638312.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT          256 a          306 c          282 g          259 t
ORIGIN      
        1 gcgttcaccg tactttcaac aggttaactc cccctttctg agaggaaaaa aattaacgca
       61 gaatcttctt ctcagccaaa tccagcgcaa agtagctgaa aatcagatcc gcacccgcac
      121 gcttaattga acctaagctt tcgagcacga ctttctcttc atctatagca cccgccagcg
      181 cggcgaactt aatcatcgcg tactcaccgc tcacctgata cgcgccaatc ggcaattcag
      241 tacgttcacg cagctcacgc acgatgtcga ggtacgctcc agcaggttta accatcaggc
      301 agtctgcgcc ctgggcttca tccagcagtg actcacgaat cgcctcgcga cggttcatag
      361 ggttcatctg atagcttttg cggtcgcctt ttaatgcgct tccggcagct tcacggaacg
      421 ggccataaaa ggaagaggcg aacttggtcg aatacgacat aatcgccgta tctttaaagc
      481 ctgcagcgtc cagcgcctga cgaatcgcct gtacctggcc gtccatcgcg gcggaagggg
      541 cgatgaagtc tgcgcctgca gcagctgcaa ccacggcttg cttgcctaaa ttttccagag
      601 tcgcgtcgtt gtcgacgcca tgctcgcaca gcacaccgca gtgaccgtga gaagtgtatt
      661 cacagaaaca agtgtctgac atgacgatca tttctggcac agtctgcttg cagatgcgcg
      721 acatacgcgc caccagtcca tcttcccgcc aggcgtcgct gccggtttca tcggtatggt
      781 gagagatgcc gaaagtcatc acggaacgaa taccggcgtt ggcgatgcgt tcaatttcgc
      841 gtgccagatg tttctctgga atgcgcatca cgcctggcat ggcttcaacg gctttgtagt
      901 cgtcaatttc ttcttcaaca aagatcggca acaccaggtc gttaaggcta agtgttgtct
      961 cttcaaacat agcgcgcagc gcaggagatt tgcgcaggcg acgagggcgt tggattaagt
     1021 ctgtcatggt ctgcctgatg tttgtggaat taaggtgcac agtatacctg aagcggggca
     1081 ggagtgtttt acgaaagttg tcg
//
LOCUS       DAJTOR010000348         1096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTOR010000348 DAJTOR010000000
VERSION     DAJTOR010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.358"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(229..305)
                     /locus_tag="OO249_004258"
     tRNA            complement(229..305)
                     /locus_tag="OO249_004258"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(269..271),aa:Arg,seq:tct)
     gene            complement(319..395)
                     /locus_tag="OO249_004259"
     tRNA            complement(319..395)
                     /locus_tag="OO249_004259"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(359..361),aa:Arg,seq:tcg)
     gene            complement(403..478)
                     /locus_tag="OO249_004260"
     tRNA            complement(403..478)
                     /locus_tag="OO249_004260"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(443..445),aa:Ile,seq:cat)
     gene            complement(528..798)
                     /locus_tag="OO249_004261"
                     /pseudo
     CDS             complement(528..798)
                     /locus_tag="OO249_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          255 a          316 c          260 g          265 t
ORIGIN      
        1 ggcgctatgt gccattgcat ggtgccgggt gcctcccggt gaattcagta ccagcacctg
       61 aatccgcgat tatcccatat acctactcgc tgattgcccc tccgcacagg gggattcacc
      121 atgccagttt cttttaacaa actccccgca aaccagacaa cagtcaaccg cctgaattgt
      181 gagacattta aaaaaaagcc cgcaaaagcg agccagggaa aataagtgtg gcgcgttgta
      241 ctggattcga accagtgacc gattgcttag aaggcaattg ctctgtccgg ctgagctaac
      301 aacgcaggat acagataatg gaccgccttc ggggacccga actccgcgca accagcttcg
      361 aaggctggcg ctctttcctg atgagctaat ggcggtatgt gatggtggcc cttgctggat
      421 ttgaaccagc gacctggcga ttatgagtcg ctcgctctca ccactgagct aaagggccgg
      481 gagcagaata ataatggtgc gtaattaatt ctgcaatctc atccgtttca aacgattaaa
      541 tcctgaactt ccctgactgt ctgctcaaaa cgtccggtct ccagttcaac gccaatcgca
      601 cgacgcccga gcgccagtgc agcttttacc gttgaacctg agcccataaa aaaaatctgc
      661 aaccaggtct cccggacgac tgcttgcgct gattatctgc tgcagcattt ctgccggttt
      721 ttcgcacgga tgtttcccgg gatagaactg caccggttta tgtgtccaca catccgtgta
      781 cggcacctgc gccgtcacac caaaataccg ccgcagatgc ttatattcac tctgcagctc
      841 cacatactgc cggttcagtg acgtatacgt atccaccagc tggtggtggg gcttttccag
      901 ttcaccgcgc tgatgtttct cttctgccac ccgggcaaac agcgactgta atttcagata
      961 atcgctttcg ttcggtagct gccactgact ggcactgaac cagtgcgaca ccatgttttt
     1021 ctttcctgtg gcatctgcaa tctgttttgc cgttatcccc agggccgcgc gcgcatcacg
     1081 aaagtaagaa atcagc
//
LOCUS       DAJTOR010000349         1078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTOR010000349 DAJTOR010000000
VERSION     DAJTOR010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.359"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>1078
                     /gene="pdeB"
                     /locus_tag="OO249_004262"
     CDS             <1..>1078
                     /gene="pdeB"
                     /locus_tag="OO249_004262"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5638313.1"
                     /translation="LPVGLSIWLAHQQVETSFIEELDTYSSRVAIRANKVATQGKDAL
                     QELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNVPQCSSLEHESPPDTFPEPG
                     KISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGN
                     AHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILPFPEMNISIITWASTKMLQK
                     GWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDAIENRDICVHYQPIVSLA
                     NGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPLTLLIIRSVFEDMGDWLRQH
                     PQQHISINLESTVLTSEKIPQLLRE"
BASE COUNT          269 a          262 c          265 g          282 t
ORIGIN      
        1 gctgcctgtc ggcttaagca tctggctggc ccatcagcag gtagaaacat cgtttattga
       61 agagctggat acctattcct cccgcgtcgc tattcgggcc aataaggtgg cgacacaagg
      121 gaaagatgcg ctgcaagagc tggaaagatg gcaaggcgct gcctgtagcg aagcccatct
      181 catggaaatg cgtcgggtat cttacagtta tcgctatatt caggaagtgg tttatatcga
      241 taacaacgtt ccccagtgtt cgtctctgga gcatgaaagt ccgcccgata ccttccccga
      301 gccaggtaaa atttcgaaag atggttatcg tgtctggtta acatcgcata acgatttagg
      361 cattatccgt tacatggtcg ccatgggaac ggcacattat gtcgtcatga tcgaccccgc
      421 ttcctttatt gatgtcattc cctatagctc atggcaaatt gatgccgcca ttattggcaa
      481 tgcccataac gttgtcataa ccagcagcga tgaaattgct cagggaatta ttaccaggct
      541 acaaaaaaca cccggtgagc atatcgaaaa taatggaatc atttacgata tcctgccctt
      601 cccggagatg aatatttcga tcatcacctg ggcttcaacg aaaatgttgc agaaaggctg
      661 gcatcggcaa gtctttattt ggttaccgct cgggttggtg attggcctgc tggcagcgat
      721 gtttgtgctg cgtattttgc gccgtatcca gtcaccgcat catcggctgc aggatgctat
      781 cgaaaatcgt gatatttgcg tgcactatca gccgattgtc tccttagcca atggcaaaat
      841 tgtcggtgct gaggcactgg cgcgctggcc gcaaacagac ggtagttggt tgtcaccaga
      901 tagttttatt ccgctggcac agcaaacagg cctttctgag ccattgacgc tactgattat
      961 aagaagcgtc tttgaagata tgggcgactg gctgcgtcag catccacagc agcatatttc
     1021 gatcaatctt gaatccaccg tgctcacctc ggaaaaaatc ccgcaattgc tgcgtgaa
//
LOCUS       DAJTOR010000350         1074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTOR010000350 DAJTOR010000000
VERSION     DAJTOR010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.360"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(15..707)
                     /gene="ospZ"
                     /locus_tag="OO249_004263"
     CDS             complement(15..707)
                     /gene="ospZ"
                     /locus_tag="OO249_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR5638314.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASIPEGSWLTV
                     ISGKRPMGQFSVDCLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RFSQIKARMSVT"
     gene            763..915
                     /locus_tag="OO249_004264"
                     /pseudo
     CDS             763..915
                     /locus_tag="OO249_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          325 a          211 c          180 g          358 t
ORIGIN      
        1 acaattaata tacattaagt aacagacatt cgagccttga tttgagaaaa cctgtcattc
       61 atttgtttta tgtagctatc tatttcctct gatgcataac taaaaagttc tcccatttca
      121 cgagcaataa taatctcaga tttaatagac tttaactctg gcgactcatc tcttaacact
      181 tgcataatct ctctttttat atcatatagt tctataaacc gatttgcccg tagttctcct
      241 tggggagaat catttctgaa tacaacaatt atatacaaaa aatcactatt ttctttaggg
      301 aagatcttac aactaatctc tgggagctca cataatgcat gtaagtcagg atggtacaaa
      361 caatccacag aaaattgccc cataggacgt tttccagata taactgtcaa ccagcttcct
      421 tccggtatac tggcttcgtc tctggaaata ggctgaaaat cttccggaca tggagaatac
      481 attgtcttca tataattaag tcgtctggat ttctcattaa taaactcctc tatttttaat
      541 tgtgccatac ttctggttat accagagata tacttcttgt catatatgtt tggtgattca
      601 ttaaagtaac catgttcaac tctaggataa atatttctca ctatatattc agtatttgca
      661 gtatttattg gaaatacgtt cttaatattc ttgatgggac taatcatttt aatctctata
      721 ctcaaatata aacattacca tgaacaatat aattgttcct aagtggaatg tctccacgga
      781 gaacacttta tctatcggga aatagtgcgg gcaacagtgt ttaattatat cgagtgcgat
      841 tacaatcgct ggcgacggca ccgttggtgt ggtggcctca gtccggaaca atttgaaaac
      901 cagaacctcg cttaggcctg tgtccatatt acgtgggtag gctcacagag cctcacttac
      961 gactgcgtcc atattatgtg ggtaggatca ataaagacgt tttatatgtg tctgcttgca
     1021 gccagaaaat ttataccctg caggtatcat aaacatgctt gataacttaa ttgg
//
LOCUS       DAJTOR010000351         1074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTOR010000351 DAJTOR010000000
VERSION     DAJTOR010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.361"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..119
                     /locus_tag="OO249_004265"
                     /pseudo
     CDS             1..119
                     /locus_tag="OO249_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            136..654
                     /locus_tag="OO249_004266"
                     /pseudo
     CDS             136..654
                     /locus_tag="OO249_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          316 a          250 c          230 g          278 t
ORIGIN      
        1 gtaagcagaa acgcaagttc agggcgacca caaactctga ccacaatctg ccggttacgc
       61 cgaatctgct gaatcagaac tttacgccca cagcacctaa tcaggtctgg gtcgcagatt
      121 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
      181 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggcg gatacaggaa
      241 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      301 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      361 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      421 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      481 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      541 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      601 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      661 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      721 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      781 tcatattctg aggattaact acataacaag gcgcaagata tatatccaaa aacaaataat
      841 accgttgcaa taattccttt cagtaaagga ggtgtgatta atttcagggg atttcagagt
      901 gaaaattgta caacatattg aaatatgttc atttcgttat aatacacaca agaaaatatt
      961 aagttagata atatattcta aataaccata gataaaaatg cacataatgg ggaatatttt
     1021 atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga tctt
//
LOCUS       DAJTOR010000352         1074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTOR010000352 DAJTOR010000000
VERSION     DAJTOR010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.362"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..447
                     /locus_tag="OO249_004267"
     CDS             <1..447
                     /locus_tag="OO249_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638315.1"
                     /translation="LPISAADKRRFYYYFQEKNTPNIERFFVFDSSEYRYALNMREVV
                     FHQFLSDGLRPIVDEDDYFNVHITLVNGGPVIPLSVEPDAPQNEETDDIGQLNAFFDA
                     LDCEPETTDRFMITDEDGEDAFIRIGSIAMVRVALDVLEPVEDDDE"
BASE COUNT          276 a          210 c          257 g          331 t
ORIGIN      
        1 ttgcccattt cagcagcgga taagcggagg ttttattatt attttcagga aaaaaacacc
       61 ccaaacatag aacgcttctt cgtttttgat tcttccgagt atcgttatgc gttaaatatg
      121 cgtgaagtgg tatttcatca gttcctttct gatggtctgc ggccaatcgt tgacgaagac
      181 gattatttta atgtccatat cacgctggta aacggcggac cagtgattcc actgagtgtt
      241 gagccagatg cgccgcaaaa cgaagagacg gatgatatcg gccagttgaa tgcatttttt
      301 gacgcgctgg attgcgagcc tgaaaccacg gatcgtttta tgataaccga cgaagatgga
      361 gaagatgctt tcattcgtat tggttctatc gctatggtgc gagttgcgct tgatgtgctg
      421 gaaccagtgg aagacgatga tgagtagtat tgaatggatt gatttcttaa gaaatggtac
      481 tgagtataca tgtgagtata caaagtaact tcgtattttt aaaaatccat tgatttcata
      541 ttgttgagtc tggaatcgcg gtgcggcctt cgcaccacac cgtatgcaaa tcgagtcgct
      601 caagagcggc tttttttgtg tctgaaatca cccatatccg tgcagtatat aggcctgcca
      661 gaaaagcagt tcagtattct tcattttgta gtcgatggtc ccaagtttgc tctggctgga
      721 agctgcatcg tccacattac gtgggcaggg tggtttcgct atgctacttg aacgttttga
      781 ccatgtggct caggtagcta ataccaaaag acgtcatttt gtctccacgc ccaccttggt
      841 gaacattcaa taaatagttt cggtcgagag cagttatgta gggctagcag acgagatgcg
      901 cagacatctt gttgaagaca attttactga ccacctgaga acgctattct accgaataat
      961 atttattatc ttggcgaata atgatgatta cctgtgtaac tatggtttta tgctgatggg
     1021 accggcagta caatttctgt agtgtactcg caatttcagg tctctggcgt ttat
//
LOCUS       DAJTOR010000353         1043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTOR010000353 DAJTOR010000000
VERSION     DAJTOR010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.363"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..82)
                     /locus_tag="OO249_004268"
                     /pseudo
     CDS             complement(1..82)
                     /locus_tag="OO249_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            48..833
                     /gene="lafU"
                     /locus_tag="OO249_004269"
     CDS             48..833
                     /gene="lafU"
                     /locus_tag="OO249_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5638316.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHIDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
BASE COUNT          289 a          242 c          278 g          234 t
ORIGIN      
        1 ccagggtaaa gcgggctgaa acctcggaaa tacgctcggc ccctttggtg acgacgataa
       61 agttgattgt caacagcgtc agtaaatccg agcgtgaaag cattatcgcc gcgctgcacg
      121 ggcagtcgat ttttaacggt ggtgggctgt cgccgttaaa taaaatcagc ccgtctcatc
      181 cgccaaaacc tgccaccgtg gcggtgccgg aagaaacgga gaaaaaagcg cgcgacgtca
      241 atgagaaaac ggcgctgctg aagaagaaat ccgccaccga gcttggcgag ctggcaacca
      301 gtatcaccac cattgcccgt gatgcgcata tggaagcgaa tctggagatg gagattgttc
      361 cccagggatt acgcgtgctg attaaagacg accagaatcg caatatgttc gaacgcggca
      421 gcgcgcagat tatgccgttc tttaaaacgc tgctggtgga gctcgcgcca gttttcgact
      481 cgctcgataa caaaattatt attaccgggc atatcgatgc gatggcttac aaaaacaata
      541 tctacaacaa ctggaacctt tcgggtgacc gcgcgctttc ggctcgtcgg gtgctggaag
      601 aggccggaat gccggaagat aaagtgatgc aggtaagcgc aatggcggac cagatgctgc
      661 tggattccaa aaatccgcaa agcgcgggca accggcgcat tgagattatg gtgctgacca
      721 aaagtgcgtc cgatacgctg tatcaatact ttggtcagca tggggataaa gtggtgcagc
      781 cgctggtgca aaagctggat aagcagcagg tgctttcgca gcgaacgcgt taaatgctga
      841 atctttacgc atttctcaaa ctctgaaatc actgtatact ttaccagtgt tgagaggtga
      901 gcaatgcgta aaatcatgat tttcacttcc taaaacaccc cttataatct ggtcatagag
      961 tcattatagc actggatgaa tatacagatg aggaaggttt acagttacat tcgcttttcg
     1021 tcaacgaaac aagcttttgg tga
//
LOCUS       DAJTOR010000354         1039 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTOR010000354 DAJTOR010000000
VERSION     DAJTOR010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.364"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..142)
                     /locus_tag="OO249_004270"
     CDS             complement(<1..142)
                     /locus_tag="OO249_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638317.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKD"
     gene            complement(126..353)
                     /locus_tag="OO249_004271"
     CDS             complement(126..353)
                     /locus_tag="OO249_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5638318.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            430..837
                     /locus_tag="OO249_004272"
     CDS             430..837
                     /locus_tag="OO249_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5638319.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
BASE COUNT          300 a          266 c          208 g          265 t
ORIGIN      
        1 agtccttgcc tggtaccaga ggcaattcgc cgcccccctg ccgcagatat tcttcagtaa
       61 ttgcgttagc gacccacgcc tgcccttttt cggctgccag ggctaacagc actgattcga
      121 tgtgctcatg cctgattttc atgaatcacc ttcccaggtc gttttgcctt acgatattcg
      181 tcataaactt tggggtcgta ctgaagttcc ccgccagatg cctcttgcag gcgcatcgcg
      241 cgaccttcag gaaccagttc cccccatgca gcaatgcttg ccagcctaac tcctgcggca
      301 ttggcaagct ttgttttgct gccaaaaaac gctatagcat caattttcaa catatcgagc
      361 tccttagatt ttcctaagga aactagatcg tagagaaacc taagtcaaga aagattagaa
      421 ttccctaata tgaaaaacga aaccttcggt gctcgcctct tacataggcg taaaaaatta
      481 aaactgtctc aggccgcatt aggtaagctg gtcaaagtgg ctcacgtaac aatttctcaa
      541 tgggaaagag atgaaacaca gccggcgggg aaaagattat tcgcactgag ccaagcgctt
      601 cagtgctcac cgacttggct tctttttggg gatgaagata aacaaccagg cgaaccgatc
      661 ccggataatc agccagccat tctgacagaa gatcaaaaag agttacttca actgttcgac
      721 gcactgcctg agtcagagca aaaggccctg ttgtcagaga tgcgtgctcg agttgaaaat
      781 ttcaacaaac tttttgaaga actactcaaa gctcgcaaaa gaagcgcaaa caaataatcc
      841 ccttttttct ccacaccctg taataaaaag cacaaacttt caaacacttg tgttttttac
      901 atcaaaaaaa cttaggtttt ttatacataa aatcttgact ccacgtctta ggttattcta
      961 aattccattc atcaagacac cgcacggtgt tctcagcaaa cagttccact accccggcgt
     1021 taaggggaaa tgaggtcag
//
LOCUS       DAJTOR010000355         1033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTOR010000355 DAJTOR010000000
VERSION     DAJTOR010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.365"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            200..560
                     /gene="yeaR"
                     /locus_tag="OO249_004273"
                     /pseudo
     CDS             200..560
                     /gene="yeaR"
                     /locus_tag="OO249_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
     gene            564..746
                     /locus_tag="OO249_004274"
     CDS             564..746
                     /locus_tag="OO249_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCR5638320.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            894..>1033
                     /locus_tag="OO249_004275"
     CDS             894..>1033
                     /locus_tag="OO249_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5638321.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WI"
BASE COUNT          277 a          231 c          260 g          265 t
ORIGIN      
        1 gcccgactgg cgacgctgca atcctgatac attcagcccg cgttgtcgcg ggcttcccac
       61 ttgtaatcct ccctgatttt tctctgatat ggtgctaaaa agtaaccaat aaatggtatt
      121 taaaatgcaa attatcaggc gtaccctgaa acggctggaa taaaccgttt tcagcgcatt
      181 caccgaagga gggaaaagga tgctgcaaat cccacagaat tatattcata cgcgctcaac
      241 gcctttctgg aataaacaaa ctgcgcctgc cggaataatt cgaacgtcat cttgataaag
      301 gaacgcgcca ggggggttac ccacgccttt ccgttatgca tggggcggtc aaatatctcg
      361 gctacgctga tgaacacagt gcagagcctg atcaggtgat ccttatcgaa gcggggcagt
      421 ttgcggtgtt ccctccagaa aagtggcaca acattgaagc catgactgac gatacttatg
      481 tcaacattga cttcttcgtg gctcctgaag tcctgatgga aggtgcgcaa caacggaaag
      541 tcattcataa cgggaaatga atcatgggca aagcaacgta taccgtgacc gtcaccaata
      601 acagtaatgg cgtttctgtc gattatgaaa cagagacgcc gatgactttg ctggtgccag
      661 aagtggcggc tgaagtgata aaagatctgg tgaataccgt gcgttcttat gacacggaaa
      721 acgaacatga tgtttgtggt tggtaattat cactgatcgt tgatggcgca atttctgcgc
      781 catcgcaaaa aaatcctccg tgatgctcgt cacaaatctt gccctcccga tgttgtgcta
      841 tgctttttat cagcgactaa cccgctaatg caaaacagat aagtgaggaa acaatgggca
      901 ttctgtcatg gattattttt gggcttattg ccggtattct ggcgaagtgg atcatgccag
      961 gtaaagatgg tggtggattc tttatgacta tcctgctggg gatagtcggt gccgtagtcg
     1021 gcggatggat cag
//
LOCUS       DAJTOR010000356         1032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTOR010000356 DAJTOR010000000
VERSION     DAJTOR010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.366"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..1032
                     /locus_tag="OO249_004276"
     rRNA            <1..1032
                     /locus_tag="OO249_004276"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          253 a          242 c          325 g          212 t
ORIGIN      
        1 actccgtgcc agcagccgcg gtaatacgga gggtgcaagc gttaatcgga attactgggc
       61 gtaaagcgca cgcaggcggt ttgttaagtc agatgtgaaa tccccgggct caacctggga
      121 actgcatctg atactggcaa gcttgagtct cgtagagggg ggtagaattc caggtgtagc
      181 ggtgaaatgc gtagagatct ggaggaatac cggtggcgaa ggcggccccc tggacgaaga
      241 ctgacgctca ggtgcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg
      301 ccgtaaacga tgtcgacttg gaggttgtgc ccttgaggcg tggcttccgg agctaacgcg
      361 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
      421 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      481 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      541 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      601 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      661 aactggagga aggtggggac gacgtcaagt catcatggcc cttacgacca gggctacaca
      721 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      781 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      841 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      901 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      961 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
     1021 gatcacctcc tt
//
LOCUS       DAJTOR010000357         1028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTOR010000357 DAJTOR010000000
VERSION     DAJTOR010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.367"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            72..953
                     /locus_tag="OO249_004277"
     CDS             72..953
                     /locus_tag="OO249_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5638322.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCIEECFLPG"
BASE COUNT          253 a          217 c          283 g          275 t
ORIGIN      
        1 ccgacacaga acatttaaaa aaatgaatgt ttttatattc tagattcgat attttcgata
       61 ggttcggggt tatggatctg cgccgtttta ttacgcttaa aaccgtggtg gaagagggtt
      121 cctttttgcg agcttcgcaa aaattgtgct gtacacaatc gacggtgact tttcatattc
      181 agcagcttga gcaggagttt tcagtccagt tatttgagaa aattggccga cggatgtgcc
      241 ttacccgcga aggaaaaaag ttgctgccgc acatttatga acttacccga gtgatggata
      301 cgcttcgtga ggctgccaaa aaggggtcag atccggacgg ggaactacgc gttgtttcgg
      361 gcgaaacgtt gctctcttac cgaatgccgc aggtgttgca gcgttttcgg caacgcgcac
      421 caaaagtgcg tttatcgttg cagtcgctga actgttacgt gatccgtgat gcgctactga
      481 atgatgaggc cgatgtcggc gtcttttatc gcgtagggaa tgatgatgcc ctgaaccgac
      541 gagagttggg tgaacaatcg ctggtgctgg tggcttcacc gcaaattgca gatgtcgatt
      601 ttactgagcc gggaagacat aacgcctgta gctttattat caacgaaccg caatgtgtct
      661 tccggcagat atttgagagc acgctgcgtc agcggcggat cacggtggaa aacaccattg
      721 agctgttaag cattgaaagt atcaaacgtt gtgtcgcggc gaatatcggt gtcagttact
      781 tgccccgctt tgcggtggaa aaagagcttg agagcggaga attaattgaa ttgccttttg
      841 gcgaacagtc gcagaccatt acggctatgt gcgcacacca tgctggaaaa gcggttagcc
      901 cggcgatgca cacttttatt cagtgtattg aagagtgttt tttgccggga taaaaataat
      961 gccgggctta aacccggcac ccaacttgtc gacgaagtgc gttttgttca tgccggatgc
     1021 ggcgtgaa
//
LOCUS       DAJTOR010000358         1028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTOR010000358 DAJTOR010000000
VERSION     DAJTOR010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.368"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..823
                     /gene="fliG"
                     /locus_tag="OO249_004278"
     CDS             <1..823
                     /gene="fliG"
                     /locus_tag="OO249_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5638323.1"
                     /translation="FEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     VATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            816..>1028
                     /gene="fliH"
                     /locus_tag="OO249_004279"
     CDS             816..>1028
                     /gene="fliH"
                     /locus_tag="OO249_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="binds to and inhibits the function of flagella
                     specific ATPase FliI; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5638324.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEADPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQG"
BASE COUNT          244 a          272 c          308 g          204 t
ORIGIN      
        1 gtttgagcaa gaagctgaac agtttgccgc actgaatatc aacgccaacg attatctgcg
       61 ttcggtattg gtcaaagctc tgggtgagga acgtgccgcc agcctgctgg aagatattct
      121 cgaaactcgc gataccgcca gcggtattga aacgctcaac tttatggagc cgcagagtgc
      181 cgccgatctg attcgcgatg agcatccgca aattatcgcc accattctgg tgcatctgaa
      241 gcgcgcccag gccgccgata ttctggcgtt gttcgatgaa cgtctgcgcc acgacgtgat
      301 gttgcgtgtc gccacctttg gcggcgtgca gccagccgcg ctggcggaac tgaccgaagt
      361 actgaatggc ttgctcgacg gtcagaatct caagcgcagc aaaatgggcg gcgtgagaac
      421 ggcagccgaa attatcaacc tgatgaaaac tcagcaggaa gaagccgtta ttaccgccgt
      481 gcgcgaattc gacggcgagc tggcgcaaaa aattatcgac gagatgttcc tgttcgaaaa
      541 tctggtggat gtcgacgatc gcagcattca gcgtctgttg caggaagtgg attccgaatc
      601 gctgttgatc gcgctgaaag gggccgagca gccactgcgc gagaaattcc tgcgcaatat
      661 gtcgcagcgt gccgccgata ttctgcgcga cgatctcgcc aaccgtggtc cggtgcgtct
      721 gtcgcaggtg gaaaacgaac agaaagcgat tctgctgatt gtgcgccgcc ttgccgaaac
      781 tggcgagatg gtaattggca gcggcgagga tacctatgtc tgataatctg ccgtggaaaa
      841 cctggacgcc ggacgatctc gcgccaccac aggcagagtt tgtgcccatg gtcgagccgg
      901 aagaaaccat cattgaagag gccgacccca gccttgagca gcaactggcg caactgcaaa
      961 tgcaggccca tgagcaaggt tatcaggcgg ggattgccga aggtcgccag caaggtcatg
     1021 agcagggc
//
LOCUS       DAJTOR010000359         1028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTOR010000359 DAJTOR010000000
VERSION     DAJTOR010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.369"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..695)
                     /locus_tag="OO249_004280"
     CDS             complement(<1..695)
                     /locus_tag="OO249_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5638325.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          302 a          171 c          220 g          335 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaaatc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac gttaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagttct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gggggggatt atttacagga agcataataa cctcgcagaa ggatatcctg
      721 ataaaatgtg atactgggaa ataataagct aaaataaaat gtttcaggat tgaaatttat
      781 tgttttcatt ttaaggaagt gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 catatttgaa tgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc
      961 gtaaaggaaa ccgtagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc
     1021 cctgaagc
//
LOCUS       DAJTOR010000360         1015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTOR010000360 DAJTOR010000000
VERSION     DAJTOR010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.370"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..274
                     /locus_tag="OO249_004281"
     CDS             <1..274
                     /locus_tag="OO249_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="oxidoreductase, Fe-S subunit; terminal electron
                     transfer protein for the reduction of DMSO; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HCR5638326.1"
                     /translation="YNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELR
                     KKHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            276..>1015
                     /gene="dmsC"
                     /locus_tag="OO249_004282"
     CDS             276..>1015
                     /gene="dmsC"
                     /locus_tag="OO249_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR5638327.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQL"
BASE COUNT          191 a          241 c          315 g          268 t
ORIGIN      
        1 atataacgaa acgaaaggcc atatgaccaa atgcgatggt tgttatgacc gtgttgcgga
       61 gggtaaaaag ccgatctgtg ttgaatcttg tccgctgcgg gcgctggatt tcggccctat
      121 cgacgagctg cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc cgcgagctca
      181 ctttaccaaa ccgaatattg tgatcaaacc caatgccaat agccgcccga ccggggatac
      241 cactggctat ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg catgaatggc
      301 cgctgatgat cttcacggtc ttcgggcaat gtgtagcagg tggttttatc gttctggctt
      361 tggcgctgct caaaggcgac ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt
      421 ttggtttatg ggtgctgatg ggcattggct ttatcgcctc tatgctccat cttggttcac
      481 caatgcgcgc ttttaactcg ctcaaccggg taggggcttc agcactcagt aacgaaatcg
      541 ccagcggttc gatctttttt gccgtaggcg gcatcggctg gttgctggca atgctgaaaa
      601 agctgtcacc ggcattgcgt acgctgtggc tgatagtgac gatggttctt ggcgtcatct
      661 ttgtctggat gatggtgcgt gtgtataaca gcattgatac cgttccgacc tggtacagca
      721 tctggacgcc gatgggcttc ttcctgacga tgtttatggg cggcccgtta ctgggttacc
      781 tgttgttgag tctggctggc gtcgatggct gggcgatgcg tctgctgcca gcaatttctg
      841 tactggcact ggtagtgagt ggcgtggtgt cagtgatgca gggcgcagag ctggcgacta
      901 ttcatagttc tgtgcagcag gccgcagcgc tggtgccgga ctatggtgcg ctgatgtcct
      961 ggcggatcgt gcttttggcc gttgccctgt gcttgtggat tgcaccacag ctaaa
//
LOCUS       DAJTOR010000361         1009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTOR010000361 DAJTOR010000000
VERSION     DAJTOR010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.371"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO249_004283"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO249_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5638328.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO249_004284"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO249_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5638329.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGTPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..>1009)
                     /locus_tag="OO249_004285"
     CDS             complement(941..>1009)
                     /locus_tag="OO249_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5638330.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          301 a          205 c          231 g          272 t
ORIGIN      
        1 gctaaagttc tgggttaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataatcat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaac ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaattg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaagg
//
LOCUS       DAJTOR010000362         1000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTOR010000362 DAJTOR010000000
VERSION     DAJTOR010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.372"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..182)
                     /locus_tag="OO249_004286"
     CDS             complement(<1..182)
                     /locus_tag="OO249_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5638331.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEA"
     gene            complement(354..>1000)
                     /locus_tag="OO249_004287"
     CDS             complement(354..>1000)
                     /locus_tag="OO249_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5638332.1"
                     /translation="DTALWRSGDVLLGSLLAMLFTGIWPQRAFIHWRIQLAKSLTEYN
                     RVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIAPASKETRIPKSIYEGIQTINRN
                     LVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPVFANT
                     EKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          243 a          244 c          234 g          279 t
ORIGIN      
        1 attgcttcaa cgctcatccc cggcttgatg taatcgctca ggttgtccag cagcaggacg
       61 cgcttctggt tgtcacggat ctttttctca acgtcctgaa tttcacgttg cagtttgttc
      121 ttacgacgga acagacgaac aaattccagt acgtcctgga atgaaggctt ggtagtttcc
      181 atttttatac ccctgataat gtgagagtcg gattcgttta agcaaccgct atccgttaga
      241 gccaacatta ctcatagctg ccgcagatga caatgctttt atcccttttc actatcatac
      301 cctttatcct tgctgaatcg aagcagcagc aagatgattc tgtagttcag gaattatttg
      361 cgcaaggccc ggcaaatcag attcgatagc aactcaagct gatgtgccgt ttccatgttc
      421 agccacacat aaccatagat tggtgtttcc acaaccttca ggtcatggtg gttattgagc
      481 aactgacgca gctcttccac tgcatcgttc aatttttccg tattggcaaa aaccggctgc
      541 ggattacctt cgtacaacgc atgaacaagg ctcagcagta tttgctgcat catgtgctgg
      601 gtatcacgaa gtttttgcgc gttcaataac acgaaatggc tggggcgcgt ggcccagtac
      661 gcattgattt gcaactccag catacaaacc agattgcggt taatggtctg gataccttca
      721 tatatcgatt ttggaatacg ggtttctttg ctggcgggcg caatcagccc acgcattttc
      781 acggcatcgg tcaggagttt ttgtagatgg ctttccagac gtgggcgttc gagtaagttc
      841 ggtgagaatg cagattgata gacccgatta tactcggtca gacttttcgc cagttgaatg
      901 cgccagtgga tgaacgcccg ttgtggccag ataccggtaa acaacattgc cagtaaagag
      961 ccgaggagca catcgccgct tcgccataac gccgtatcaa
//
LOCUS       DAJTOR010000363          966 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTOR010000363 DAJTOR010000000
VERSION     DAJTOR010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.373"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..483)
                     /locus_tag="OO249_004288"
     CDS             complement(1..483)
                     /locus_tag="OO249_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5638333.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCIQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="OO249_004289"
     CDS             complement(474..776)
                     /locus_tag="OO249_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5638334.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          294 a          238 c          194 g          240 t
ORIGIN      
        1 tcagacctta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctggata caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaacacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgcc
//
LOCUS       DAJTOR010000364          930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTOR010000364 DAJTOR010000000
VERSION     DAJTOR010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.374"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..476
                     /locus_tag="OO249_004290"
     CDS             <1..476
                     /locus_tag="OO249_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR5638335.1"
                     /translation="GGWNFYQYPLNPISEIDPQGLNPLILGVVALMSCGLFSSGGVIQ
                     SGQQRREQYGRKVNPEADKLTDEHKSGVNPGGNCSPQNLSDLQNEKNRLCNQSRACAP
                     QMLRKEILRRYEINLACASVRKQINNQCFGGGDKAHMEEENKAYKTAADCSGLIK"
     gene            473..>930
                     /locus_tag="OO249_004291"
     CDS             473..>930
                     /locus_tag="OO249_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638336.1"
                     /translation="MKKIIFSNESAVYDELMIHFPCQPLPHISNDIIGLNELDIVYNF
                     FQKKQWNEIANNLKIKDDSYALELGITFLPEKVFCYYIPLYIYVSLFNKNDFWVFESD
                     FIQQYLCPEYRDYDDFLNFVFNFSDIQLSIIAQFMSYESDAGFFYASKAC"
BASE COUNT          304 a          140 c          191 g          295 t
ORIGIN      
        1 aggggggatg gaatttttat cagtatccgt tgaatccgat ctcagagata gacccacaag
       61 ggttaaatcc gttaattctt ggtgttgtag cactgatgag ttgcggtttg ttttcatcag
      121 gtggtgttat ccaaagtggg caacaacgca gagagcaata tggacgtaaa gttaatccag
      181 aagccgacaa attaacagat gaacataagt caggtgtaaa tcctggaggt aactgtagtc
      241 cccagaacct gagtgactta caaaatgaaa aaaacagatt atgtaaccag tctagagctt
      301 gcgctcctca gatgctgaga aaagaaatat tgagacggta cgagataaat ttggcgtgcg
      361 cttcggtcag aaagcagatt aataatcagt gctttggtgg tggagataag gcccatatgg
      421 aagaagagaa taaagcatat aaaacagcag ctgattgcag tggcttaata aaatgaaaaa
      481 aataattttc tctaacgaga gtgcagtcta tgatgaactt atgattcatt tcccatgcca
      541 gccattacct catatttcaa atgatattat agggctgaat gaactggata ttgtatataa
      601 ttttttccaa aagaaacaat ggaatgaaat agccaataat ttaaaaataa aggatgactc
      661 ttatgcttta gagttaggaa ttacattcct ccctgagaaa gtattctgct attacatccc
      721 tctgtatatt tatgtctcgt tgtttaataa gaatgatttt tgggtttttg aatccgattt
      781 tattcaacaa tatttatgtc ctgagtatcg ggattacgat gattttttga attttgtttt
      841 taatttttca gatatacaac tatctattat tgcacagttt atgtcttatg aatcagacgc
      901 tggattcttt tatgcaagta aagcttgtat
//
LOCUS       DAJTOR010000365          928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTOR010000365 DAJTOR010000000
VERSION     DAJTOR010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.375"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>928
                     /locus_tag="OO249_004292"
     CDS             <1..>928
                     /locus_tag="OO249_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91-like element ISSbo1 family transposase"
                     /protein_id="HCR5638337.1"
                     /translation="LACGTRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATD
                     LWIATQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQE
                     PGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLL
                     KAWSEGLAMPESLSHITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKK
                     PPIAASRLAHYNGGASLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYF
                     GFLANRVCGEKLPQVYRALGMDKQEPVAKVCYA"
BASE COUNT          215 a          233 c          300 g          180 t
ORIGIN      
        1 tgctggcctg cggcacgcgg atactgggag tgaaggaata catctgcgat aaacctgagt
       61 gcccccacgt cagatacgtc actaactcat gcggcagccg tgcctgcccg tcctgcggaa
      121 aaaaggccac agacctgtgg atagcgacac agctgaatcg tcttcctgac tgcgactggg
      181 tacacctggt cttcaccctg ccggacacgc tgtggccggt gttcgaaagc aaccggtggc
      241 tgctgaatga cgtgtgccgt ctggcggtgg agaatctgct gtatgccgcc cggaaacggg
      301 ggcaggaacc cggtatcttc tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc
      361 cgcatgtaca tgtgtctgta acctgtggag gtctgaataa gcatggtcag tggaaaaagc
      421 tgagcttcct gaaagacgcg atgcgttcac ggtggatgtg gaatatgcgg cagctgcttc
      481 tgaaagcgtg gtcagagggg ctggcgatgc cggagtcgtt gtcacatatc acgacggaat
      541 cacagtggag aagcctggtg ctgaaagccg gaggaaaata ctggcatgtg tacatgtcga
      601 aaaaaacggc cggcgggagg aatacggcgc gctacctggg tcgttatctg aagaagccgc
      661 cgatagcggc ctcccggctg gcacattaca acggaggggc gagcctgagc ttccgttacc
      721 tggaccacaa aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga
      781 ggctgaaaca gcacatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg
      841 ccaaccgggt gtgtggagag aagctgccgc aggtgtaccg tgctctgggg atggataaac
      901 aggaaccagt ggcgaaagtg tgctatgc
//
LOCUS       DAJTOR010000366          914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTOR010000366 DAJTOR010000000
VERSION     DAJTOR010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.376"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>914
                     /gene="tuf"
                     /locus_tag="OO249_004293"
     CDS             <1..>914
                     /gene="tuf"
                     /locus_tag="OO249_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5638338.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT          212 a          230 c          264 g          208 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccg
//
LOCUS       DAJTOR010000367          909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTOR010000367 DAJTOR010000000
VERSION     DAJTOR010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.377"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..>909)
                     /locus_tag="OO249_004294"
     CDS             complement(<1..>909)
                     /locus_tag="OO249_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5638339.1"
                     /translation="AFLTGIAGALQTPTLSIFLADELKARPIMVGFFFTGSAIMGILV
                     SQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNRNYFILLSTGVLLSSFASTANPQ
                     MFALAREHADRTGRETVMFSTFLRAQISLAWVIGPPLAYELAMGFSFKVMYLTAAIAF
                     VVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRDTRLLFVVCSMMWAANNLYMINM
                     PLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYYMKRIGKRLLMLIAIVSGMCFYA
                     SVLMATTPAVELELQILNAIFLGILCG"
BASE COUNT          280 a          214 c          213 g          202 t
ORIGIN      
        1 ccacagagaa taccaaggaa gatggcattt agaatttgca attccagctc aaccgccgga
       61 gtcgtcgcca tgagtacgct ggcgtaaaaa cacataccac tcacgatagc aatgagcatt
      121 aatagtcgct tgccaatacg tttcatgtaa tagcctgcga ttaacatcat cggaatttcc
      181 agaccggcag cgataccaat catttctcca gccagtttat cggttagatg cagttcatca
      241 ataataaata gcggcatatt tatcatgtag agattattcg ccgcccacat cattgaacag
      301 accacaaaaa gtagccgcgt atcccgcttc ctatgggtgg agggtaaaat ttctacgggt
      361 tgggtaacga caggaatatt tctttgtatt gatggcaaaa acaaccagac tatcagcccg
      421 caaacaacaa atgcgatggc agcggtgaga tacatcactt taaaactaaa ccccattgcc
      481 agttcataag cgagcggtgg cccgataacc caggcaagcg agatctgagc acgtaaaaat
      541 gtactgaaca tgaccgtctc acggcctgtt ctgtcggcgt gttcacgggc gagggcgaac
      601 atttgcgggt ttgcggtaga agcaaaacta ctcagaagta cgcccgtaga gaggagaatg
      661 aagtagttgc gattccacgc aaaaagcgtg caggccagca ctccaaataa gcaacatagc
      721 agaatcagta atttacggtc gccttgttta tcggagtgcc ttgccagaaa ttgactgacc
      781 agaattccca taatagcgct accggtgaag aaaaaaccta ccattatagg acgggctttc
      841 agttcatctg cgaggaatat acttagggta ggagtctgaa gagcgcccgc aatacccgtc
      901 agaaaggcg
//
LOCUS       DAJTOR010000368          901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTOR010000368 DAJTOR010000000
VERSION     DAJTOR010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.378"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..322
                     /locus_tag="OO249_004295"
     CDS             <1..322
                     /locus_tag="OO249_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5638340.1"
                     /translation="VIKAVGVQRNGSEFVLLEVDASDGVKMLSTKVLSGVDSETWRND
                     FEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGVNHPKGLGELQVSREDMEGWAERVVRE
                     QFTH"
     gene            complement(378..530)
                     /locus_tag="OO249_004296"
                     /pseudo
     CDS             complement(378..530)
                     /locus_tag="OO249_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(553..807)
                     /locus_tag="OO249_004297"
                     /pseudo
     CDS             complement(553..807)
                     /locus_tag="OO249_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          237 a          169 c          257 g          238 t
ORIGIN      
        1 agtgattaaa gccgttgggg tgcaacgtaa tggcagtgaa tttgtattgc tagaggtgga
       61 tgcatcggat ggggtgaaaa tgctttctac caaagtgttg agtggcgttg atagcgaaac
      121 atggcggaat gattttgaaa agatacggcg tggagtggtg aagagctcat tgaattggcc
      181 aaatagtttg tttgatcaat tatatggaca agacgggcat agaggggtga atcatccaaa
      241 ggggttgggg gagctgcaag tatcgagaga ggatatggaa gggtgggctg agagagtggt
      301 tagagagcaa tttacgcatt aaaggaatga ctgaaagagc ctgtaaaccc ttttgtgtaa
      361 gtgcttttgc cggtcagtta aaggtggcca tttaaacggt caccaaattc gatcataaaa
      421 cggttcatgg ccggcttcca gttgcggatc ggcatcgtcc atttcttggt cgccgcctgg
      481 atagccaggt acaccacctt catcgctgat tcgtccgtag ggaacacctt gagttcatat
      541 taatggaatt ttctacaaat agcctccgtg gttttgaggg gggattacag acgatccata
      601 gtagtaatcc aatgagttct tgagcgcggc gacatgtttg gacgccttgg caaaaattag
      661 agcctgcttg aagtgcaggc gagcacgtgc ttggacgatt gatccattcg cggtcaaaaa
      721 ctcaatcttg gatgacagcg tgtcaggaaa tccaatatcg aagtcccgcc ccgtgtcaaa
      781 gtagggcatt tcatgatcaa aggatgaacc gccccgggtt tcctggagag tgttttatct
      841 gtgaacccag gctgccagat catcgtttcc gatggaagca taataagctt tttctgcttc
      901 t
//
LOCUS       DAJTOR010000369          894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTOR010000369 DAJTOR010000000
VERSION     DAJTOR010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.379"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..127)
                     /locus_tag="OO249_004298"
     CDS             complement(<1..127)
                     /locus_tag="OO249_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5638341.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHF"
     gene            415..>894
                     /gene="rutR"
                     /locus_tag="OO249_004299"
     CDS             415..>894
                     /gene="rutR"
                     /locus_tag="OO249_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5638342.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     "
BASE COUNT          225 a          197 c          249 g          223 t
ORIGIN      
        1 cgaaatggta atgctccgct ttttgcacaa tggctttatt cagttcaaag gtcggcatgt
       61 actgcggcgc gtgggtcgaa atgagccagc cgttgttgcc aataggtacg aatacgccaa
      121 ttttcatcat caacctctct tcgtctcgta aagtgaaagt cagacggggc gctgcatcct
      181 gcatatcctt ttcagccgcg tattggcttg tttgcaaagc ggatgccagt ttttaaaaag
      241 tgaatgttat taatgtgtta gcattgtgtt atctaaaata tctggtaaaa agtggactaa
      301 acggtcaaaa cagttgcaca taaaacatgc atccgtgcgc gatgagagtg cagaaggtcg
      361 aggccgggcg ggggttttgc tatcctgttg ccaatctaca agaggggaga gcgcatgacg
      421 caaggcgcag tgaaaacaac gggtaaacgt tcgcgcgcag taagcgcgaa gaaaaaagcg
      481 attcttagcg cagcactgga cactttttca caattcggtt ttcacggcac aaggctggag
      541 cagatcgcag agttggcggg tgtttcaaaa accaatctgc tgtattactt tccgtcaaaa
      601 gaggcgctgt atattgccgt gctgcggcag attctcgata tctggctggc accgttaaaa
      661 gcgtttcgtg aagatttcgc cccgctggcg gcgatcaaag agtacatccg tctgaagctg
      721 gaagtctcac gcgattatcc gcaggcttcg cgcctgttct gtatggagat gctggcaggc
      781 gcgccgctgt taatggatga actgacgggc gatttgaagg cattaattga tgagaaatcg
      841 gcgctgattg ccggttgggt caaaagcggc aaactcgcgc cgattgatcc gcag
//
LOCUS       DAJTOR010000370          877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTOR010000370 DAJTOR010000000
VERSION     DAJTOR010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.380"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            22..300
                     /locus_tag="OO249_004300"
     CDS             22..300
                     /locus_tag="OO249_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5638343.1"
                     /translation="MSDAVYRRLILMKAMSNITDCSVPDINRMLRFMFGKKRRAYVLN
                     NGGLRMSYIFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            287..>877
                     /locus_tag="OO249_004301"
     CDS             287..>877
                     /locus_tag="OO249_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5638344.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTVRDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFN"
BASE COUNT          223 a          200 c          248 g          206 t
ORIGIN      
        1 tcggataccc ggactgttga cctgtctgat gctgtatacc ggcggctgat actgatgaaa
       61 gccatgtcga acattactga ctgttccgtt ccggatatta accggatgct gagatttatg
      121 ttcggaaaaa aacggcgagc ttatgttctg aataatggtg ggctgaggat gagttacatc
      181 tttgagtccg cgctctcgtt ggcagaactg gcgattatcc agtcgtcggg tgcactgccg
      241 tccccgccgg gtgtttatgt ttcagtagtt ttaaaggagt cccgtaatga aggccagtga
      301 taaaccccgc cagctggcgg tcccctttgc gagtaccgga gataaaaacc gtatcccgga
      361 caaggcgaca cagcagacca gagagagcgg taatgctgcg tatgattcag gttttcctcc
      421 ggtgaccatg acagcggtct cagcgggagg tataccgcca cacggcaagg attttaacgg
      481 tctgatgtac gatattaccg cagcaatacg gttcgcccag gctggcggtt tgtacacgta
      541 taatgccggt tttgcggggg ccattggtgg atatgcaaaa ggagccattc tcgccggagt
      601 cgcaacaaca gcggtctggc tgaataccac agacgataac ctgaccgatc ctgaaggctc
      661 cgacagtgcg ggctgggtaa atcttcttga ggatccgaaa aggatattcc tgcggcagaa
      721 gaacaatctg tcagaccttc agaataaagg gacggtacgg gataatcttc aggtttacag
      781 taaagagcag tcagatcaac gctatgttca tcgggaaggc gataaaataa ccggagagct
      841 gaaaatccgt ggtgttaatg cgctgaggat tttcaac
//
LOCUS       DAJTOR010000371          865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTOR010000371 DAJTOR010000000
VERSION     DAJTOR010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.381"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..841
                     /locus_tag="OO249_004302"
     CDS             <1..841
                     /locus_tag="OO249_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5638345.1"
                     /translation="LVGYIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQC
                     IGVEILLRWNNPRQGRISPDVFIPIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHV
                     GINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVDYRIARELHRKNVK
                     LAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIEL
                     VAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIM
                     PLR"
BASE COUNT          200 a          220 c          222 g          223 t
ORIGIN      
        1 actggtgggc tacatcgcct ggctggcgac cgcttaccgg atgagctttt cccgcgaaat
       61 caatctgggc ctggcgcaac atgagttcga attgttctgt cagcctttgc ttaatgcgcg
      121 cagccagcaa tgtattggtg tagagatttt gctgcgctgg aacaatccgc gtcagggccg
      181 gatttcaccg gatgtgttta ttcctatcgc ggaagaacat catttaattg tgccactgac
      241 ccgctatgtg atggcagaaa ccattcgtca gcgccatgtt ttcccgatga gtagtcagtt
      301 tcatgttggc attaacgtcg cacccagcca ttttcgccgt ggtgtgctga taaaagatct
      361 caatcagtac tggtttagcg ctcacccgat tcagcaactg atcctcgaaa tcaccgaacg
      421 cgatgcctta ctggatgttg attatcggat tgcccgcgag ctacatcgta aaaacgtcaa
      481 actggcgatt gatgacttcg gcaccggcaa cagttcattt tcctggcttg aaacattacg
      541 tcctgacgtg ctgaaaattg ataagtcatt taccgcagct ataggttctg acgcggttaa
      601 ctcgacggtg accgatatca tcatcgcgct ggggcaaaga ctgaatattg aactggtggc
      661 ggagggcgtg gaaacgcaag aacaggcgaa gtatttgcgc cgtcatggcg tgcatatttt
      721 gcaagggtat ttgtacgcac agccgatgcc gctacgcgat tttcccaaat ggctggcggg
      781 cagccaaccg ccgcccgccc ggcataatgg acatatcacg cccattatgc cgttacgtta
      841 acgctgatat gactttccgt gtcaa
//
LOCUS       DAJTOR010000372          842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTOR010000372 DAJTOR010000000
VERSION     DAJTOR010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.382"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..509
                     /gene="cybB"
                     /locus_tag="OO249_004303"
     CDS             <1..509
                     /gene="cybB"
                     /locus_tag="OO249_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5638346.1"
                     /translation="QISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMIHVSCGISI
                     LVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGLVMMYNRGN
                     PWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHYFWKDNTLL
                     RMMPRKRS"
BASE COUNT          211 a          190 c          193 g          248 t
ORIGIN      
        1 tacaaatcag cattcactgg ctggtctttt tgctggttat cgcagcgtat tgcgcaatgg
       61 agtttcgggg tttcttccca cgtagcgatc ggccactcat caacatgatt catgtttcct
      121 gcggcatctc aattctcgtg ctgatggtcg ttcgtctttt gttaaggctg aaatacccaa
      181 ccccgccgat tatacctaag ccaaaaccga tgatgacggg actggcgcat ttgggacatt
      241 tggtgattta tctcctgttt attgcgctgc cagtgattgg tttggtgatg atgtataacc
      301 ggggcaaccc gtggtttgcg tttggtttga cgatgcctta cgcttcagag gccaatttcg
      361 aacgggttga tagcttaaag tcgtggcatg aaacgctggc gaatctggga tattttgtca
      421 tcgggttgca cgctgcggct gcactggcac accactattt ctggaaggac aacacacttc
      481 tacgcatgat gccgcgtaaa cgttcctgaa ggatatttaa agaaaacgcc tgtactaaaa
      541 ccgacccgtg gtacaggcga agaatacggg tctacatcgg aagcgcctat tatatttatt
      601 tgtatgacaa ataaaaccat aatccttgcc catacgtcca tctggcttat ttttaatcaa
      661 ttcacccgat ctttgatctc atcaacggta tcaaaataaa ccagcgtata acgttcaaat
      721 cgaacataag attcgataaa ccatggaggt tatatgaaaa aactggcact tattttgttt
      781 atgggaacgc ttgtttcctt ttatgccgat gccgggcgca aaccctgttc tggttcgaaa
      841 gg
//
LOCUS       DAJTOR010000373          796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTOR010000373 DAJTOR010000000
VERSION     DAJTOR010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.383"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            21..796
                     /locus_tag="OO249_004304"
                     /pseudo
     CDS             21..796
                     /locus_tag="OO249_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          230 a          186 c          197 g          183 t
ORIGIN      
        1 caggacgaag tgggcgtcgt gtgcgtaaac aattcgccac caaaggcgaa gcactggctt
       61 ttgagcgcca cacgatggaa gagaccgaag caaagccctg gctgggtgaa tcagtggatc
      121 gtcgaacact gaaagacgtg gttgagctat ggttcaaact acatggtaaa tctctgacag
      181 ctgggcagca tgtctatgac aaattgctgt tgatggttga cgctctgggc aatcctcttg
      241 caaccgatct cacctctaaa atgtttgccc actatcgaga taaacgcctg acaggcggga
      301 tctacttcag cgagaaatgg aagaaaggag caagcccggt caccattaac ctggagcaaa
      361 gctatctaag tagtgttttt agcgaactat cccgtctggg cgaatggtcg tatccgaacc
      421 cactggagaa catgcgaaaa ttcaccatcg cagaaaaaga gatggcatgg cttacccatg
      481 agcagattgt tgaattgctg gctgattgca aacgtcagga cccaattctg gcactggtag
      541 ttaagatatg cttaagcaca ggcgcacgct agcgtgaagc cgtaaatctt acccgctcac
      601 aggtgaccaa ataccgaatt acctttgtca gaacgaaggg gaagaaaaac agaagcatcc
      661 ctatcagtaa agagctttac gaagagatca tggcgcttga tgggttcaat ttcttcacag
      721 actgctattt tcaattttta tccgtgatgg aaaaaacgtc tatcgtgctc cctcgcggtc
      781 aactcacaca cgttct
//
LOCUS       DAJTOR010000374          764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTOR010000374 DAJTOR010000000
VERSION     DAJTOR010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.384"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..648
                     /gene="ospC2"
                     /locus_tag="OO249_004305"
     CDS             <1..648
                     /gene="ospC2"
                     /locus_tag="OO249_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5638347.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFINNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLRNGAVSGKRFGR"
BASE COUNT          279 a           99 c          151 g          235 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat atcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattaa taatggattg gttgatgtaa acaaaagatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aagattcaaa aacaattgat
      601 tttttattaa gaaatggagc ggtatcaggc aaacgatttg ggaggtgacc taaaatatat
      661 ttaggttgtg ccctgaatct gacgttgtca tttttgttat attgatcgat ataggggatg
      721 gtgcagaccg ttcccctgca acaacacatt gcatgtggtg aacg
//
LOCUS       DAJTOR010000375          739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTOR010000375 DAJTOR010000000
VERSION     DAJTOR010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.385"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..386
                     /locus_tag="OO249_004306"
     CDS             <1..386
                     /locus_tag="OO249_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638348.1"
                     /translation="GIGWQFSRRTPPELKNRLHCWLVFSPVYFWLILSNFILYISPEK
                     SALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGFALGYYCHWCRKNRNQR
                     KKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(438..>739)
                     /locus_tag="OO249_004307"
     CDS             complement(438..>739)
                     /locus_tag="OO249_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5638349.1"
                     /translation="RRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEF
                     YEAELKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
BASE COUNT          164 a          190 c          177 g          208 t
ORIGIN      
        1 ctggcatcgg ttggcaattc tcccgcagaa cgcctcctga attaaaaaac agactgcatt
       61 gctggctggt cttttcgccg gtctatttct ggttaattct ctcgaatttc attctttata
      121 tttctccaga gaaatcagcg ttgctggaaa atatccgaaa tttctttctg acgtttgtct
      181 ggcctcccct gaatttttcc cctttttggc cgcagccttg gactgatttt gtcggcccga
      241 ttagtgcaca gcttggtttt gcattgggat attattgcca ttggtgcaga aaaaatagaa
      301 accagaggaa gaagtggggc gattgggtaa cgtgcttaag tttggcgatt ttagctcagg
      361 ggccgttatt caattattta caatgaatag tctaaggaat ccaaatagcc cggttgcact
      421 tcaaccgggc atcatcctta cgacgccagc gacaacttct gctgcgtata ttccaccagc
      481 cactgactca cacgagattg ttgatccgca ttcagccaca tgccttgttt tgtgcgacgc
      541 caaagggcat cgtcggtgcg gcggacccat tcgtgatcca ccaggtattt cagttccgct
      601 tcgtagaact catgaccgaa atcctccccg aggtcgctta tcgcccccgc attgccgagc
      661 agcagctcgc tgttgctgcc gtaagtgcgt gcgtaatgac gcgccagcga ttcagtcagg
      721 aatggatagc gacggcgca
//
LOCUS       DAJTOR010000376          683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTOR010000376 DAJTOR010000000
VERSION     DAJTOR010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.386"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..316)
                     /locus_tag="OO249_004308"
     CDS             complement(<1..316)
                     /locus_tag="OO249_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="catalyzes the conversion of 5-methoxyuridine to
                     uridine-5-oxyacetic acid in tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5638350.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MISMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAY"
     gene            complement(357..>683)
                     /locus_tag="OO249_004309"
     CDS             complement(357..>683)
                     /locus_tag="OO249_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5638351.1"
                     /translation="LRLQYRVAYGDGGFSELQSAIRIHGNAVEYIPIAIVLMLFMEMN
                     GAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRSGMSATWCALLLMVLANLWYMPWE
                     LVFSLR"
BASE COUNT          213 a          177 c          169 g          124 t
ORIGIN      
        1 tataggcgtc aatatgacga cggcagcgtt caatcatcgc cggggagttg tcgatggcaa
       61 taattttgca attatcatga tgaatgttgc gacgcaccga gagcgtcgcc gcgcccagag
      121 aacaacccag atcgtaaacc tgcgtaccag gttgaacgaa gcgctcggct aacatactaa
      181 tcatggaaat aatattggaa tagccgggaa cggaacgctg gatcatatcc gggaagactt
      241 cagctacccg ttcatcaaag gtccagtcgc ccagtctggc gataggggca gaaaatagcg
      301 tgtcgcggtg agacataacg taaaaatccg ggaaaaagaa agtggcgtat tgtgcgctaa
      361 cgcagggaga aaaccaactc ccagggcata taccaaagat tcgccagcac catcagcaac
      421 agcgcacacc aggtggcgct catgccggaa cgtcgccagc gaaacagacg gtgatgaaaa
      481 ccgtaataat gcatcagacg accagcaagc aaaacgatgc cgcaaatatg caccatccag
      541 gtttctgcgc cattcatttc cataaacagc atcagcacaa tcgcgatggg aatatattcc
      601 accgcgttac catgaatgcg aatagcgctt tgcagttcgc taaaaccacc gtcaccatag
      661 gcaacgcggt actgcaaacg caa
//
LOCUS       DAJTOR010000377          673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTOR010000377 DAJTOR010000000
VERSION     DAJTOR010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.387"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            193..462
                     /locus_tag="OO249_004310"
     CDS             193..462
                     /locus_tag="OO249_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638352.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..673
                     /locus_tag="OO249_004311"
                     /pseudo
     CDS             459..673
                     /locus_tag="OO249_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          168 a          154 c          197 g          154 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaa
//
LOCUS       DAJTOR010000378          643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTOR010000378 DAJTOR010000000
VERSION     DAJTOR010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.388"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            88..>643
                     /gene="ytfB"
                     /locus_tag="OO249_004312"
     CDS             88..>643
                     /gene="ytfB"
                     /locus_tag="OO249_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCR5638353.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     EFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVV"
BASE COUNT          172 a          173 c          178 g          120 t
ORIGIN      
        1 aataaaggcg ggtttaccac ggacatcata gcgttagctt attcataggc tatgattgag
       61 gaacaagacg cggagcagga ggaaaccatg cccgggcgct ttgaactaaa accaaccctg
      121 gagaaagtct ggcacgcgcc ggacaatttt cgcttcatgg acccgctgcc gccgatgcat
      181 cgtcggggga tcattattgc cgccattgta ttggtggtcg aatttctgct tccatctgat
      241 gatacgccca acgcgccggt ggtgacgcgt gaagcgcagt tagatattca gtcgcaatca
      301 caaccgccga cagaagaaca gctgcgggcg caattagtca caccacaaaa tgatcctgat
      361 caggtggctc cggtcgctcc agaacctata caggaaggcc agccagaaga gcagccgcaa
      421 actacccaaa cgcaaccatt ccagccagat agcggaattg ataatcaatg gcgctcttac
      481 cgtgtcgaac ccggtaaaac gatggcgcag ctgttccgcg accacggact gcccgctacc
      541 gatgtctatg caatggcgca ggtagaaggc gcgggtaagc cgctgagtaa tttgcaaaac
      601 gggcagatgg tgaaaatccg ccagaacgcc agcggcgtgg tga
//
LOCUS       DAJTOR010000379          639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTOR010000379 DAJTOR010000000
VERSION     DAJTOR010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.389"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..>639)
                     /locus_tag="OO249_004313"
     CDS             complement(<1..>639)
                     /locus_tag="OO249_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR5638354.1"
                     /translation="EMKRLNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKG
                     LFWGAAEGGPIETGEIPPGNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMT
                     QMNIPRVKEFGVPSTGGNTCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDY
                     GLWCNYGQLHRDFTYCYSKGVFKCVLPAEEYAEIHWDQLEAGDVNFIKDFYYR"
BASE COUNT          168 a          172 c          134 g          165 t
ORIGIN      
        1 acggtagtaa aaatctttaa tgaaattaac gtcacccgct tccagttgat cccagtgaat
       61 ttccgcatac tcttcagcag gcagaacaca cttgaacacc cctttgctgt aacaataagt
      121 aaagtcgcga tgcaactgcc cgtagttaca ccataggccg tagtcatcga acaaattcag
      181 accaaccaag ttaccaatca cacggccatc gtctttatct tcaaaatctt tcggaccgtt
      241 cgggaagatg gtggtatgga caaagttggc tacacaagtg ttaccacctg tgctgggcac
      301 gccaaactct ttcacccgag gaatattcat ttgggtcata caacggatcg ggcaagagtg
      361 gcagccactc atttttactg tgtatttctc tgccgccgga cctaagtcaa aaacggattt
      421 ataggtacga aagccgaccg tgttctgatt gcctggcgga atttcccccg tttcaatcgg
      481 accaccttca gccgcgcccc aaaacagccc tttacgtgct gtccaacgtg acttgggatc
      541 tgaatactct gcccacgatt gcggcgtact tggcacgaca tggttgttat tcgcaccaat
      601 aagttcagtc atcatgtaat cgttgagacg cttcatctc
//
LOCUS       DAJTOR010000380          638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTOR010000380 DAJTOR010000000
VERSION     DAJTOR010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.390"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>638
                     /locus_tag="OO249_004314"
     CDS             <1..>638
                     /locus_tag="OO249_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5638355.1"
                     /translation="NGVESWCFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEIN
                     AMLPSTTAISHASFQEKEAGDGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIE
                     AEPTWISFSRPSRIEKSRRARKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADV
                     GGKQDATNYNSIFANRFAAFDELLSILKTKFACRVLFEETLVLPKVGRSRLH"
BASE COUNT          201 a          112 c          158 g          167 t
ORIGIN      
        1 gaacggcgtt gaaagctggt gctttagctt tgaccctccg cccatgcagg gttggaaatt
       61 acatgtaaaa ggacgttctt ctaacgagga taaggattat ttagttgagg aaatagtagg
      121 tttagaaatc aacgctatgc ttcctagcac aacagctatt agccatgcct cttttcagga
      181 aaaggaggca ggtgatggta gtacgcagca catagcggtt tcaacagagt cagttgttga
      241 tgatgagcat ctacagttgg acgatgagga aacagccaat atagacacag acacacgagt
      301 catagaggct gagccgacat ggataagttt tagtagacct agtcgaattg aaaaatctcg
      361 cagggcaaga aaaagtagcc aaactatttt agaaaaagaa gaagcaacaa caagtgaaaa
      421 tagtaatttg gttagtactg atgagccaca cttaggtggt gtcctagcag cggcagatgt
      481 gggtgggaag caggatgcaa ccaattacaa ctctattttt gctaatcgat ttgctgcttt
      541 tgatgagcta ctttcaattc taaaaactaa atttgcatgt cgggtgcttt ttgaagaaac
      601 cttggttttg ccaaaagttg ggcgtagccg attacatc
//
LOCUS       DAJTOR010000381          631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTOR010000381 DAJTOR010000000
VERSION     DAJTOR010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.391"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            106..>631
                     /locus_tag="OO249_004315"
     CDS             106..>631
                     /locus_tag="OO249_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR5638356.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEH"
BASE COUNT          162 a          170 c          167 g          132 t
ORIGIN      
        1 cctaaatagc tgcgcggaat agtagatccc tttgagggaa cttagcccgg attgtgcgat
       61 ctgatcaatc gccaaatcaa aacaaatcac caaccggact gagcaatgcc gatcatagca
      121 ccaatttccc gtgacgaacg acgcctgatg cagaaagcca tccataaaac acacgataaa
      181 aattatgccc gcagactgac tgccatgctg atgctgcacc ggggcgaccg tgtcagcgac
      241 gttgccagaa cgctctgctg cgcccgttcc tctgttggac gctggattaa ctggttcacg
      301 cagtcgggtg ttgagggact gaaatcatta cctgccgggc gtgcccgtcg ctggccgttt
      361 gagcatatct gcacactgtt acgtgagctg gtaaaacatt ctcccggcga ctttggctac
      421 cagcgttcac gctggagtac agaactgctg gcaataaaaa tcaatgagat aaccggttgc
      481 cagttaaatg ccggaaccgt tcgccgctgg ttgccgtctg cggggattgt gtggcgaagg
      541 gctgcgccaa ctctgcgtat ccgtgacccg cataaagatg aaaagatggc agcaatccat
      601 aaagcactgg acgaatgcag cgcagagcat c
//
LOCUS       DAJTOR010000382          620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTOR010000382 DAJTOR010000000
VERSION     DAJTOR010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.392"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(126..572)
                     /locus_tag="OO249_004316"
     CDS             complement(126..572)
                     /locus_tag="OO249_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5638357.1"
                     /translation="MNDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          171 a          153 c          128 g          168 t
ORIGIN      
        1 tacctgttga taataaggta tcgggtaact ctcattatga cagagcgctt tcaaaaacag
       61 ggctacgaaa tggcgagcat ccagacgtat attaagaaag cccccatcaa catctattaa
      121 atttatcact gcgcccagtc aggtttattt aagatatgat cctgccagtc aatcacttct
      181 gattctttca ctgcgatatg acgcacagag atacgttcac catgcattgc cgctttcgaa
      241 ccagtaagta gcggatgcca cacaggtaaa tctttacctt ccgccagcaa acgataagcg
      301 caggtcattg gcagccattc gaatgttggc agattttcac gggttaattt aatgcagtcg
      361 ggttcaaact cgaaacgacg ttcgtagttc cgacattgac aggttttaat attgagctgg
      421 cgacaggcga cgttagtgaa gtagatttcg tcggtgtctt catccatcag tttatgcagg
      481 caacactgac cgcaaccatc acacaacgac tcccattccg catcgctcat ttcgtccagg
      541 gttttacttt gccagaaagg tacatcgttc atcaggtgtt ccgccattac gttaaaacgc
      601 accttataac cagtctggca
//
LOCUS       DAJTOR010000383          613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTOR010000383 DAJTOR010000000
VERSION     DAJTOR010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.393"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            10..564
                     /locus_tag="OO249_004317"
     CDS             10..564
                     /locus_tag="OO249_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5638358.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          178 a          110 c          175 g          150 t
ORIGIN      
        1 aggttattta tgatttaccc aacaaataca ggcaaaagcg gggaacacct tcgtctcacc
       61 acgctggaaa gtgtctggat tcagggaaaa ctgcgcatgt gggggcgctg gtcgtatatt
      121 ggcggcggta agacggggaa tatgttcaac cagttgttgg cctctaaaaa gctgacaaaa
      181 acggcaatta acgaggcgct ccggaggatg aaaaaagcag gtctgaacaa gtctgaactt
      241 gaggcttttt tgcgggatat gattaacggt aagcaaaaga gctggctggc gcattgtact
      301 gatgcagagg cgttatgtat tgatcgggtc ataagtgagg tgctggcaga gcatccagga
      361 ttgattagcg tccttcggca acggtatgag gggcggggga tgaccaaacg caaaatggct
      421 gaactgctga atgatgcaca cccgaaatgg agtttaagaa cctgtgaaag acgcattgag
      481 cattggctaa aggtggcaga atttattttg tacaaaccaa tggttatggc ttttggtata
      541 gagaaaaaag ttattgcttt ttgacgtaaa aactgcttca attcttgtac gcttcgcaaa
      601 gctgtaccgc gag
//
LOCUS       DAJTOR010000384          606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTOR010000384 DAJTOR010000000
VERSION     DAJTOR010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.394"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..213)
                     /locus_tag="OO249_004318"
     CDS             complement(<1..213)
                     /locus_tag="OO249_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5638359.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEK"
     gene            complement(288..431)
                     /gene="mgrB"
                     /locus_tag="OO249_004319"
     CDS             complement(288..431)
                     /gene="mgrB"
                     /locus_tag="OO249_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5638360.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
BASE COUNT          187 a          151 c          114 g          154 t
ORIGIN      
        1 tttctctggt tcgtttgctt cgcacaaacg acgtaacagt gcattttgac gtttttgctg
       61 atccagcaac gcctcaagca gttcaatctg ttcgttagtc cgtgaactgg cacgattgat
      121 aaaaaaccac aagatgagcc cgataagaag aactaccacc gatacaacca aagacgcaat
      181 gttcatcacg cctgaattta caacttcgtt catttcacca cctctgagta gaggcgctat
      241 tctaccactg ctggagagga agaaaatcta gtgctgaaaa aatgatatca ccacgggata
      301 aactggttaa tggcacaaat tccgctgaaa aattgtacat cctgatcgca catcatgttg
      361 aatacctgcg cccaaagcag caagcaagcc aacaccacga caaccagaac gacccatcga
      421 aactttttca ctccaatctc cgcttcacct accttatgtc attaaactag catgcgttag
      481 ttaaacagcg cctaactatg tcgatattcg tgctttttcg gaatgagtag cttgcttcag
      541 cgcatattgc cgctacgatt aagcgaacat aaaaagagaa gaggttgtaa tgcgattcat
      601 cattcg
//
LOCUS       DAJTOR010000385          594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTOR010000385 DAJTOR010000000
VERSION     DAJTOR010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.395"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..593)
                     /locus_tag="OO249_004320"
     CDS             complement(<1..593)
                     /locus_tag="OO249_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5638361.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDS"
BASE COUNT          188 a          137 c          108 g          161 t
ORIGIN      
        1 tacgaatcca taacatcttg atccgaaaac aaccaaacca aaagctgcac tacggccgac
       61 tgctctaacg cccctttagg aaacgatgaa ctgggtaaga tccttatctc taaatgatct
      121 cgctcaacct catactgaac gtcgaactct tgctgtaaag cggtactgct caaacagctt
      181 cgacagaaat aggagttaat taaaaataat gaccgagcta gctctaactg tgggatgtga
      241 taacgagtct tgctatatgg tgtttcagct tcgtaaataa atgaactctg cgctccatcc
      301 ttgttgcgta cggaagcaaa ggacgagaga ttaggaaaat cgattatttt ggctcgttga
      361 agatccgatg cttcaaactc aatcacacga tccgccttat ttatttgctt tgttgtactg
      421 ttaaccacct tagctcgact taataaaggc aaatgagtaa atcgagtcca tttccgttct
      481 tgcattggat taaaccaaac aaaaatacgc cactccttat gacccgagtt acggaataaa
      541 tgaccaatat gtacaacctg cacattgtca ttaaatgtag ctagcctaac cact
//
LOCUS       DAJTOR010000386          592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTOR010000386 DAJTOR010000000
VERSION     DAJTOR010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.396"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            312..592
                     /locus_tag="OO249_004321"
                     /pseudo
     CDS             312..592
                     /locus_tag="OO249_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          156 a          148 c          150 g          138 t
ORIGIN      
        1 acgggaatat ctgcggcagc gggcttgtaa tgggttaagt gataacagat gtctggaaat
       61 ataggggcaa atccacatcc ccgccagccc ggctaagatg gaggcacggc acagcccgca
      121 cacgacgctg aggctacaga gctgaaaacc cacaaggcca tgccccgcat ggccgggtaa
      181 agcggagccg gcggcgactt cctttgtgct ctgtccggga ccggcaggaa aaaggcgggg
      241 ataatgagat ggtcaccccc accctaacag cagtacgctg ttaaggtgaa tcccttaacg
      301 gagagaccat catacagaac atcacactta ttggtatcga tctcggaaag cattccttcc
      361 acattcattg tcaagacaag tctggtaagg ctcttctgcg taaaaaattt acccagtact
      421 aaactgatgc agtttctggc aacatgtccg ccctcagttg ttgtaatgga agcctgtgcg
      481 ggtgcacact ttttggctcg ccgtatctct gaattttgga catgaaacta agctgatttc
      541 tccacagttc gttcgcccgt ttgtcaaaag taataagaat gattttgttg at
//
LOCUS       DAJTOR010000387          592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTOR010000387 DAJTOR010000000
VERSION     DAJTOR010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.397"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(270..>592)
                     /locus_tag="OO249_004322"
     CDS             complement(270..>592)
                     /locus_tag="OO249_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5638362.1"
                     /translation="GNKKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLS
                     KDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKNPKMIDFLLKNGAILGK
                     RFEI"
BASE COUNT          170 a          120 c          101 g          201 t
ORIGIN      
        1 cgtaaagaca ggagtcaagt tcccgctccg acataactgc gtgcaatgga ttttgaagga
       61 accgtcagcc cattccccgg aggcaaacat gggctggcaa aagtgtagcg gtattcggca
      121 cagctattta ggaaagaggg accatctcca tctcattaaa tggaccacgc cagacaattc
      181 actgtcatgc tgatgctgca ctgtggggac agaatcactc atgatgactt cgataatcga
      241 cgacattatt atttggccga gcttttaggt catatctcaa atcgtttgcc taatattgct
      301 ccatttttta ataaaaaatc aatcattttt ggatttttgc ttttcattgc attgtcaagc
      361 atagtgtccc cactatttgc tttttgaaat tttttgttta catcaaccaa tccattattg
      421 ataaaatatt ctagcacttt atagttggca ccatctttac taagtaagta ttcaacatca
      481 tacagattac aatatctatt gttgttcatt ttctccatct ctgcgacatc ttgttttgta
      541 aaattaaatt tagatagtaa ataaagtgct atatcagctt ttttattgcc ta
//
LOCUS       DAJTOR010000388          592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTOR010000388 DAJTOR010000000
VERSION     DAJTOR010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.398"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>592
                     /locus_tag="OO249_004323"
     CDS             <1..>592
                     /locus_tag="OO249_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR5638363.1"
                     /translation="YGFIAGTDFIRVENLSTPKRASAKTRQQIEHDYLLSLDMAKEVA
                     MVERNEQGRAIRRYFIQCEEELQRSVPEIAARYRRQLKARLSAANNFKPMCDALNMAR
                     AEMGKTTQQRHYSNESNMIARIVLGGMTAKQWAQANGITGEPRDSMNAAQLEHLAYLE
                     STNITLIDMGMEYEQRKGELTRLSQRWLAKRLEVNHA"
BASE COUNT          164 a          157 c          161 g          110 t
ORIGIN      
        1 tacggtttta ttgccggaac cgattttatc cgtgttgaaa atttgagcac gccaaaacgg
       61 gcgagcgcaa aaactcgcca gcaaatagag catgattacc tgctatcgct ggatatggct
      121 aaagaagtgg caatggttga acgcaacgag cagggccgcg ctatccgccg ttatttcatc
      181 cagtgcgagg aagaattaca gcgtagcgtg cctgaaatcg ccgcccgcta tcgtcgccag
      241 ctaaaagccc gtctcagtgc cgcaaacaac tttaagccaa tgtgcgatgc gctgaatatg
      301 gcccgtgccg agatgggcaa aacgacgcag cagcgccact acagcaacga gagcaacatg
      361 atcgcccgta tcgtgctggg tggcatgacg gcgaagcagt gggcgcaggc aaatggcatt
      421 actggcgaac cacgggacag catgaacgca gcacagcttg aacacctcgc ctatctcgaa
      481 agcaccaata tcacgttaat tgatatgggc atggaatatg agcagcgcaa aggagaactc
      541 acccgcctgt cgcaacgctg gctcgctaaa cgtctggagg taaaccatgc gt
//
LOCUS       DAJTOR010000389          591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTOR010000389 DAJTOR010000000
VERSION     DAJTOR010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.399"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>591
                     /locus_tag="OO249_004324"
     CDS             <1..>591
                     /locus_tag="OO249_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5638364.1"
                     /translation="PGLAMRRIADLHAGEAKTDARDAAIIAEAARTLPHALRTLKLAD
                     EQIAELSMLCGFDDDLAAQTTQASNRIRGLLTQIHPALERVLGPRLEHPAVLDLLQRY
                     PSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLA
                     LQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGV"
BASE COUNT          125 a          198 c          154 g          114 t
ORIGIN      
        1 tccctggact ggccatgcgc cgcatagccg acttacacgc cggtgaagct aaaactgatg
       61 ctcgtgacgc tgccatcatt gccgaagctg cccgtaccct gcctcacgcg ctacgcacgc
      121 tgaaactggc tgacgagcaa atcgccgaac tctccatgct ctgcggcttc gatgatgatc
      181 ttgccgcaca gacaacgcag gccagcaacc gtatccgcgg ccttctgacc cagatacatc
      241 cggcactgga gcgcgttctc ggtccgagac ttgagcaccc ggcggtactc gatcttctcc
      301 agcgatatcc ctcaccagaa aaactcgctt cgctgggtga gaagaagctg gcagcccagc
      361 tctgcaaact tgcgcctcgt ctgggtaaac gccttgcagc agacatagct caggcactgg
      421 ccgaacaaac cgtcgtcgtt cccggcacga atgccgctgc cgtagtactg ccacgtctgg
      481 cactccagct catcacgctg cgtaagcaaa gagacgaggt ggcgcttgag gtagaacagc
      541 gagttcttgc tcaccctctt tacccggtcc tgaccagtat gcccggagtc g
//
LOCUS       DAJTOR010000390          586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTOR010000390 DAJTOR010000000
VERSION     DAJTOR010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.400"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..96)
                     /locus_tag="OO249_004325"
     CDS             complement(<1..96)
                     /locus_tag="OO249_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5638365.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDI"
BASE COUNT          165 a          150 c          103 g          168 t
ORIGIN      
        1 aatatcgcga aacgccgtta ccagatcatc ctggtagacg atatcggagg ggatctcacg
       61 acgaataatt ttgctgaata tagtttcttc tgccacgaca ttttcctttt tcaaatagcc
      121 atgcgttgcg ccatgctacg ccgcgccaca ctgcgagtat agagtatggt cgagttaaac
      181 ctgctctttc aaccttaact cacgatttct taagcaaaaa atcgttttgc tgccgtatat
      241 atcgccatta ttcccatttc tgcctgcgaa tgcgctgaaa agacattctc tgtttggttt
      301 acacgacaaa tatgaatgcg tatatttctc atttgcattt acaaacaaaa ttattcgcac
      361 actaaatata atcgctcatc accctcctcc ggcatgagcc tgacgacaac ataaaccatg
      421 agatttcaaa tgctttcaac acaatttaac agggataatc aatatcaagc catcaccaaa
      481 ccgtcactac ttgccggttg catagcactg gcactattac cttctgccgc ttttgctgca
      541 tcagccactg aagaaacggt gattgttgag ggttcagcca cagctc
//
LOCUS       DAJTOR010000391          559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTOR010000391 DAJTOR010000000
VERSION     DAJTOR010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.401"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>559
                     /locus_tag="OO249_004326"
     CDS             <1..>559
                     /locus_tag="OO249_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5638366.1"
                     /translation="LDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLTQLNVS
                     YNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPELLETLD
                     VSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLPENLRN
                     GYFDINQISHIPESILNLRNECSIDI"
BASE COUNT          177 a          131 c           81 g          170 t
ORIGIN      
        1 ctagacttgt ctgaattagg cctgacaaca ttacctgaaa tccccccggg aattaaatca
       61 attaatataa gtaaaaataa tttaagctta atctccccat tgcctgcgtc ccttacacag
      121 cttaatgtca gctataacag acttattgaa ctgcctgctt tgcctcaagg acttaaatta
      181 ttgaatgcgt cccacaatca actaatcaca ctacccacac tccccatatc tttgaaggag
      241 cttcatgtct caaataatca attatgttct cttcctgttt taccagaact actggaaaca
      301 ttagatgtat catgtaatgg gctggcagtt ttaccacctt taccattttc tttacaagag
      361 attagcgcaa tagggaatct tcttagtgaa ctcccccctc tacctcacaa cattcactcc
      421 atatgggcaa tcgacaatat gttaaccgat attccatacc tgccggaaaa tttaaggaac
      481 ggttattttg acataaatca gataagtcat atcccggaaa gcattcttaa tctgaggaat
      541 gaatgttcaa tagatatta
//
LOCUS       DAJTOR010000392          557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTOR010000392 DAJTOR010000000
VERSION     DAJTOR010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.402"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..557
                     /gene="treA"
                     /locus_tag="OO249_004327"
                     /pseudo
     CDS             1..557
                     /gene="treA"
                     /locus_tag="OO249_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
BASE COUNT          142 a          137 c          137 g          141 t
ORIGIN      
        1 agcctgtatc tttttgtgtt tcgctgcgct atcggtgcag gcagaagaaa caccggtaac
       61 accatagccg cctgatattt tattagggcc gctgtttaat gatgtgcaaa acgccaaact
      121 ttttccggac caaaaaacct ttgccgatgc cgtgccgaac agcgatccgc tgattatcgg
      181 atgcagcaaa accagagcgg atttgatctg cgccatttcg ttaacgtcaa tttcaccctg
      241 ccgaaagaag gcgagaaata tgttccgcca gaggggcagt cactgcgcga acatattgac
      301 ggactttggc cggtattaac gcgttctacc gaaaacaccg aaaaatggga ttctctgtta
      361 ccgctgccgg aaccttatgt cgtgccgggc ggacgttttc gcgaggtata ttactgggac
      421 agttacttca ccatgttagg acttgctgaa agcggtcact gggataaagt cgcggatatg
      481 gtggccaatt ttgctcatga aatagacact tacggtcata ttcccaacgg caaccgcagt
      541 tactatttaa gccgctc
//
LOCUS       DAJTOR010000393          533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTOR010000393 DAJTOR010000000
VERSION     DAJTOR010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.403"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..533
                     /locus_tag="OO249_004328"
                     /pseudo
     CDS             1..533
                     /locus_tag="OO249_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT          170 a          108 c          144 g          111 t
ORIGIN      
        1 gcatatggtt cggcaaataa gacgattatt aaagataaag gaacaatgtc agttttaacg
       61 aatgctaaag ctgatgcgac ccgaatagat aacggcgggg taatggatgt taccggaaac
      121 gcgaccaata ccataatcaa tggtggtaca cagaatatta ataatcatgg tattgccacg
      181 ggtaccaata tcaacagcgg aacacaaaat atcaagagcg gcgggaaagc tgacacgaca
      241 aatatttcca ccgggagccg gcaggttgtt gagaaagatg gtacggcaac tggcagcaat
      301 attagcgccg gaggctcgct gattgtctat accggcggta tagcacatgg ggttaaccag
      361 gagacgggca gtgctttagt tgccaacacg ggcgcaggga ctgatatcga aggatacaac
      421 aagctctctc acttcactat tacccggagg ggaggctaat tatgttgtgc tggaaaatac
      481 cggcgaactg acggtagtgg ctaaaacctc ggcgaaaaat actaccgttg atg
//
LOCUS       DAJTOR010000394          532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTOR010000394 DAJTOR010000000
VERSION     DAJTOR010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.404"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..405
                     /locus_tag="OO249_004329"
     CDS             <1..405
                     /locus_tag="OO249_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5638367.1"
                     /translation="TSDISSGEYAVMPLAPMKESDAPNKELRQAWEYYHTPRAQYPTA
                     PGYATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYH
                     IVEGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            405..>532
                     /locus_tag="OO249_004330"
     CDS             405..>532
                     /locus_tag="OO249_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5638368.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGS"
BASE COUNT          148 a          127 c          128 g          129 t
ORIGIN      
        1 accagcgata ttagcagcgg agaatatgcc gttatgccac tggcaccaat gaaggagtcc
       61 gatgcgccga acaaagaact gcgtcaggcc tgggagtatt accacacccc tcgcgcgcag
      121 tatccaacag caccgggtta cgctactttg cgcagcctta accagattat tacctttgat
      181 gcttaccata tggcggaagt gtacctgact cagccgatgc agattgtggc gggcagccag
      241 gcagggagca aatggatgag tgacgatctg tacgatcgag catcaagcca agataaacgc
      301 tatcacattg ttgaaggcgc aaaccatatg gatctttatg acggcaaagc ctatgtggct
      361 gaagcaattt cagtattagc tccgttcttt gaggaaactc tgtaatgaaa catattcaaa
      421 tccgcaatag tgatatggcc tggcatattg ctgccaatat tcagtttcca ccaaattttg
      481 atgagtcaaa acagtatccg gcgattatca gcgtccatcc ttttggcagt tg
//
LOCUS       DAJTOR010000395          532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTOR010000395 DAJTOR010000000
VERSION     DAJTOR010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.405"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..>532)
                     /locus_tag="OO249_004331"
     CDS             complement(<1..>532)
                     /locus_tag="OO249_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR5638369.1"
                     /translation="FSGEITGVTDGAGRHFQLVLTTQAQRAEEARQQAISGGTEPSAF
                     PDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNT
                     QVRSFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITI
                     TDSLNRREVLHTQGEAG"
BASE COUNT           85 a          169 c          161 g          117 t
ORIGIN      
        1 ccggcttcac cctgcgtgtg cagcacttca cggcggttca ggctgtcggt gatggtgatg
       61 cggtccttct catactgata cgtgtagctt aagcctgccg ggttaagctg ttctgtcacc
      121 cgcccgtcgc tgtcgtaacg gtaacggatt tccggccggc ccgtgtgacg gtgcgccacc
      181 atccggcccc ggtatttatc atcgtaagta aagctacgca cctgtgtatt gctgcggtca
      241 tacactgccg ccagttcgcc gcgcggcgtc cagccatagc gcaccagcgg cgcggcaggt
      301 aaattctccg ggtattccgg gtcgtgcgtc agccacacgg cagacagacg gatgccgttg
      361 tcccggccat attcggtgta acccggcagg gtatcaggaa aagcggacgg ttccgtcccg
      421 ccggaaatgg cctgctgccg ggcttcttct gcccgctgcg cctgcgtggt cagtaccagt
      481 tggaagtgac gcccggcacc atccgtcacg ccggtgattt cgccgctgaa tt
//
LOCUS       DAJTOR010000396          520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTOR010000396 DAJTOR010000000
VERSION     DAJTOR010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.406"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            151..486
                     /gene="iraM"
                     /locus_tag="OO249_004332"
     CDS             151..486
                     /gene="iraM"
                     /locus_tag="OO249_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5638370.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          207 a          101 c           70 g          142 t
ORIGIN      
        1 ggataaacta caagacgaga tataaaccac cctgcattta aataaacaat aaacaacatc
       61 agaaaaataa ttctgctcta tggtttacat tcaaaaatat catttatact tttcagaaca
      121 tcaccagcaa ggcataaaca aggaaactaa atgaagtgga ttgtgattga tacagttatc
      181 cagccatcat gcggaatatc tttttcagtc atatggagta aaataaaatt aataatctgg
      241 tatcaatcgg atgctttctt acctcctgaa agtatattta cactgactca cacaggcatc
      301 atgctcaata acaaagtgct acctgtaacc atttacaacg tagtaccatt caataaaaca
      361 ttctggaatt taatcaaaaa cagccaggaa tgccctacaa atacagataa cgtattgaat
      421 gaatgcttta ataaccgttg cactctgcaa atatgtcctt atggactaaa acaacaaagt
      481 ccataaggag tttactcaca tctgacaaaa tcaatataaa
//
LOCUS       DAJTOR010000397          520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTOR010000397 DAJTOR010000000
VERSION     DAJTOR010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.407"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>520
                     /locus_tag="OO249_004333"
     CDS             <1..>520
                     /locus_tag="OO249_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR5638371.1"
                     /translation="LYSLVMSFLWSNTSFGIHRFKVKLDTAYCIKYAILAFLALLPFL
                     AVAGYIIFEQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTV
                     SLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQ
                     AKNTYLLGDLDDL"
BASE COUNT          137 a           84 c          114 g          185 t
ORIGIN      
        1 tttatatagc ctggtaatga gttttctctg gagcaatacc agtttcggta tacatcgttt
       61 caaggtgaaa ttagatactg cgtattgtat aaaatatgcc atcctcgcat ttttagcttt
      121 attacctttt ctcgctgttg ctggttatat tatcttcgaa caaatattaa atgcgtatga
      181 tagttctgtg tatgcaaatg atgatattga gaatttacag caatttatgg aaatgcaacg
      241 taaaatgata atcgcgcagt taatctatta ttttgggatt gctgttagca ccagttattt
      301 aacggtgtcg ttgcgaaatc attttatgag taacctgtca ctgaatgatg ggcgtattcg
      361 ttttcgctca actttaacgt accacggtat gctttatcgc atgtgtgcgt tggtagtgat
      421 atccgggatt acgggcggtc tggcttatcc actgctgaaa atatggatga ttgactggca
      481 ggcaaaaaat acgtatttgc tgggcgattt ggatgacctt
//
LOCUS       DAJTOR010000398          519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTOR010000398 DAJTOR010000000
VERSION     DAJTOR010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.408"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            226..302
                     /locus_tag="OO249_004334"
     tRNA            226..302
                     /locus_tag="OO249_004334"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:260..262,aa:Arg,seq:tct)
     gene            complement(348..>519)
                     /locus_tag="OO249_004335"
     CDS             complement(348..>519)
                     /locus_tag="OO249_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5638372.1"
                     /translation="RMRYAVQMLLMDNKNITQVAQLCGYSSTSYFISVFKAFYGLTPL
                     NYLAKQRQKVMW"
BASE COUNT          132 a          126 c          117 g          144 t
ORIGIN      
        1 gagcggacaa ttttccgtta gggcggtaaa ttgtactttg atacatgaaa atacgggttt
       61 tcttgattca gacgcgcagc ggtgtgcgtt tgtttgccgc tatagcgaaa taaatcagaa
      121 aatcagacgc ggtcgttcac ttgttcagca accagatcaa aagccattga ctcagcaagg
      181 gttgaccgta taattcacgc gattacaccg cattgcggta tcaacgcgcc cttagctcag
      241 ttggatagag caacgacctt ctaagtcgtg ggccgcaggt tcgaatcctg cagggcgcgc
      301 catttcttct tcatttatgc ccgtcttatc cgtttccgct ttgcccttca ccacatcact
      361 ttttgtcgct gtttggcgag ataattcaac ggtgtcaggc cgtaaaacgc cttaaaaaca
      421 gagataaagt acgacgtgct gctatagcca cataattgcg ccacctgagt gatatttttg
      481 ttatccatca ataacatctg tacggcgtaa cgcatacga
//
LOCUS       DAJTOR010000399          518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTOR010000399 DAJTOR010000000
VERSION     DAJTOR010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.409"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..488
                     /locus_tag="OO249_004336"
     CDS             <1..488
                     /locus_tag="OO249_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5638373.1"
                     /translation="KYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIAHPGNINAW
                     DNEDFVKAVKATGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRH
                     SAWDRMSQAGAQLMTWFGVACELHRDWHNDIEGLATLFSNHIPDYRNLMTCYDTLTKQ
                     K"
BASE COUNT          140 a          118 c          126 g          134 t
ORIGIN      
        1 ctaagtattt caacttacca actattctta ccaccagttt tgaaaccggt cctaacggcc
       61 cattagttcc agaactgaaa gcacaattcc ccgatgcacc ttacattgct cacccgggaa
      121 atattaacgc ctgggataac gaagattttg taaaagctgt caaagcgaca ggtaaaaaac
      181 agttaattat tgccggtgtg gtaaccgaag tttgcgtggc attcccggcg ctttcagcca
      241 ttgaagaagg gtttgacgtc tttgtcgtga ctgacgcgtc agggaccttt aatgaaatta
      301 cccggcattc ggcatgggat cgcatgtcgc aagctggtgc gcaattgatg acatggttcg
      361 gcgtggcgtg cgagctgcat cgcgactggc ataatgatat tgaaggactg gcgacgttgt
      421 tctctaacca cattcctgat tatcgtaatt tgatgacctg ttatgacacg ttaacgaagc
      481 agaaataaaa gagaaccggg gccgaaaccc cggttgtt
//
LOCUS       DAJTOR010000400          516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTOR010000400 DAJTOR010000000
VERSION     DAJTOR010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.410"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..>516)
                     /gene="yliI"
                     /locus_tag="OO249_004337"
     CDS             complement(<1..>516)
                     /gene="yliI"
                     /locus_tag="OO249_004337"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCR5638374.1"
                     /translation="VRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQGMAMNPWSNA
                     LWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAGTEQPVFYWK
                     DSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRILTDRGQRIR
                     DVRTGPDGYLYV"
BASE COUNT          115 a          148 c          112 g          141 t
ORIGIN      
        1 acgtataaat aaccgtcggg tccagtgcga acatcacgaa ttcgctgccc tctgtccgtt
       61 aaaatacggc catcttctgt cactttgtcg ccgttgacgc tcatcacaat gacatcttta
      121 tctttcagcg cgccaataaa taatttttgc tgccactggg ggaatttatc gctgttatag
      181 aaggccatgc cgctcacagc gggcgaatct ttccagtaaa aaacaggttg ctcggtcccg
      241 gcgacgatct cccctttcgc ttccggtatc ttaaagcctg aatagttgat tccccaggtt
      301 gccagcggcc agccgtagtt tttgcctttt tgcgggatat taatttcatc accaccgcgc
      361 gggccatgtt cattcagcca cagtgcatta ctccacggat tcatcgccat tccttgcgga
      421 ttacgaatgc cataagacca gatctcggcg cgcgcaccgg attcctttat aaaaggatta
      481 tcatccggga tttcgccctg atcggtcaga cgcacc
//
LOCUS       DAJTOR010000401          516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTOR010000401 DAJTOR010000000
VERSION     DAJTOR010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.411"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..281
                     /locus_tag="OO249_004338"
     CDS             <1..281
                     /locus_tag="OO249_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5638375.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          126 a          115 c          139 g          136 t
ORIGIN      
        1 atagcctgaa cgtgtactgt cgtaatggcc aggtggaaat cgacaacaac atcggtgaaa
       61 acgcgctgcg atcggtggcg gttggaagaa aaaattatct cttcttcggt tctgacaacg
      121 gtggagaaag cgcggcgatt atctacagtc tgctggtcac ctgcaaactg aacggagtgg
      181 agccggagga ctggttgcgc gaagtgatcg tgaagctcaa tgactggccg tcgaaccgag
      241 tgcatgaact gctgccctgg aacttctcag ctgtaaaata atccttccgc tacgtacttc
      301 tctgggcgct taccactcaa cggcatcgtc aaaattatca aagcgcagaa catagattta
      361 tcatgtgttt tatggatggc tttctccatc aggcatcgtt cgtcacggga aattggtgct
      421 atgatcggca ttgatcagtc cggttggtga tttgttttga tttggcgatt gatcagatcg
      481 cacaatccgg gctgagttcc ctctaagtga tctagg
//
LOCUS       DAJTOR010000402          504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTOR010000402 DAJTOR010000000
VERSION     DAJTOR010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.412"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            342..>504
                     /locus_tag="OO249_004339"
     CDS             342..>504
                     /locus_tag="OO249_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5638376.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAALNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGN"
BASE COUNT          126 a          111 c          113 g          154 t
ORIGIN      
        1 ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc agtggtacgt
       61 ctgaaatcga tcagacgcga tgcattgctc tgaaagcata gacgggaaat atgagtttgc
      121 tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag ttcaataaaa
      181 acaatcagat gagactaatc atctcttcag cattagcgtt atgaatgagt aatataaatc
      241 gataataccg ccgctgggat atcgcgtatt ttcactcatt gacaatgttt tggcggtggc
      301 atgatgcgca tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccggc
      361 ctgtcatgct tttgctgtct ggcctgcttt tgttgactct ggcgattgcg gcgttaaata
      421 cactcgtgcc gctttggctc gcccaggaac acatgtccac atggcaggta ggcgttgtca
      481 gctcatccta ttttaccggc aacc
//
LOCUS       DAJTOR010000403          492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTOR010000403 DAJTOR010000000
VERSION     DAJTOR010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.413"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            122..492
                     /locus_tag="OO249_004340"
                     /pseudo
     CDS             122..492
                     /locus_tag="OO249_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          193 a           86 c           68 g          145 t
ORIGIN      
        1 ggctattgtg cgctcataca ctcaaattaa agataggttc taaataaatg agcgtttttt
       61 gatagtctat ttcattaggt aatatatatt tgtaacaaat caatcaaaat ggaataaaat
      121 catgctacca tctatttcaa tcaacaatac cagcgcaact tacccagaat ccattaatga
      181 aaataacaat gatgaagtta atggattagt acaagagctc aaaaaccttt ttaatggtaa
      241 ggaataagca cctgtattaa acatctactt gagcttataa aaaacgccat acgagtaaac
      301 gacgatcctt atagatttaa tattaataat tcctcagtta cttatattga tattggctcc
      361 aatgatacag accatattac tattggtatc gacaaccaag aaccaataga attacctgcg
      421 aactataaag acaaagaact cgtccgtact atcattaatg acaacatagt tgagaagact
      481 catgatatca at
//
LOCUS       DAJTOR010000404          491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTOR010000404 DAJTOR010000000
VERSION     DAJTOR010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.414"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            20..364
                     /locus_tag="OO249_004341"
     CDS             20..364
                     /locus_tag="OO249_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR5638377.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            415..>491
                     /locus_tag="OO249_004342"
     CDS             415..>491
                     /locus_tag="OO249_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5638378.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPE"
BASE COUNT          138 a           98 c          137 g          118 t
ORIGIN      
        1 gcgcggggag agtaaagcga tgaagaaaaa atacgaactg gttgttaaag ggataaataa
       61 ttacccgaat aagattactg ttactgtggc actggaaatt ggtgggtatc cgtcactgtt
      121 gttgccagat gtggcgatta gtcttgaccg tactgaagat gccacgctgg agttttacga
      181 agctgaggcg aaaaagcagg cgaagcagtt tttcatggat gttgctgccg ggttatgtga
      241 aggggatggt ccgttgccgg aaaagcgccc cgtaatttta gaggcgcagg atgtgttgat
      301 aacctacaga ggaaaactac cgggaataat tactggttct ctgaagactc caccgctggc
      361 ctgaagactt aacatatcca gggatttgaa atcgataaac cctgataaat atccatgaac
      421 accaaaatca aatacggcct gtcggctgcc gttctggcgc tgattgccgc aggtgcgcct
      481 gcgcctgaaa t
//
LOCUS       DAJTOR010000405          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTOR010000405 DAJTOR010000000
VERSION     DAJTOR010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.415"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..>489)
                     /gene="capU"
                     /locus_tag="OO249_004343"
     CDS             complement(<1..>489)
                     /gene="capU"
                     /locus_tag="OO249_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5638379.1"
                     /translation="NSKIAFEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAI
                     VCHSGHDSNIVGLVRFFTWKHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTH
                     LEQEGCRTRVTVVPPGFDFQELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKG
                     HEF"
BASE COUNT          150 a          110 c           93 g          136 t
ORIGIN      
        1 aaattcgtgc cctttttccg ggcgcaacat tcctacctga gcaattacgg ggcatcctct
       61 tcgggacgcc agccaggaaa gaacactcgg aggcaaactg tttcgggaat caacgtataa
      121 ttcctggaaa tcaaagccag gaggcacaac agtaacccga gttcgacatc cttcctgctc
      181 cagatgtgtc ttcatatttg ttccgggaac aatcacctca tcgcaaaaat gatttattga
      241 aaaaaatttt gtttttcgtg tcaaatatgt cttttgcctg ataattctga atggatgttt
      301 ccaggtaaaa aaccgtacca aaccaacaat attactatca tgtccactgt gacaaacgat
      361 tgcatcgggc tgaaaactat gaactattcc gagtaatctc catacagtag gaatgtgaag
      421 actgtttcga aataacgcga atgtgatatc aattccaaac ttactggctt caaaagcaat
      481 tttgctgtt
//
LOCUS       DAJTOR010000406          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTOR010000406 DAJTOR010000000
VERSION     DAJTOR010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.416"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>489
                     /locus_tag="OO249_004344"
     CDS             <1..>489
                     /locus_tag="OO249_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5638380.1"
                     /translation="GKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQN
                     RAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQ
                     SCGKWYISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTAFEPVNSFQKNQKKLA
                     RL"
BASE COUNT          133 a          120 c          129 g          107 t
ORIGIN      
        1 cgggaaaatg gcttcctggc tggttgagtg gaaaaatgcc actgaaacgc aatggcttaa
       61 agatgccccg tcacagccat tgcaacagtc actgaaagac cttgagcggg cttacaaaaa
      121 cttcttccag aatcgggctg cttttccccg attcaaaaag cggggacaga atgatgtatt
      181 ccgctacccg cagggcgtta agctcgatca ggaaaacagc cgtatttttc tgccgaaact
      241 gggctggatg cgctaccgga acagccgtca ggtcacgggt gttgtgaaaa atgtcactgt
      301 cagccagtcc tgcggtaagt ggtacatcag tattcagaca gaaagtgaag tatcaactcc
      361 ggttcaccct tcagcatcaa tggtcgggct ggatgctggc gtggctaaac tcgccacgct
      421 gtcagatggc acagcctttg agcctgtaaa cagttttcag aaaaaccaga agaagctggc
      481 gagacttca
//
LOCUS       DAJTOR010000407          485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTOR010000407 DAJTOR010000000
VERSION     DAJTOR010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.417"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(2..313)
                     /locus_tag="OO249_004345"
     CDS             complement(2..313)
                     /locus_tag="OO249_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5638381.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
BASE COUNT          100 a          132 c          134 g          119 t
ORIGIN      
        1 gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat accatcacac
       61 cttctgccag taatgcctgt cgctgacgct gtaaatccgg tccggttagc gaaattgtgc
      121 cgtggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga cgctttaaca
      181 caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca tcaccgtaag
      241 tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg ggaaatgaat
      301 cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga aaaacgcccg
      361 agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc gatgggttgc aattagccgg
      421 ggcagcagtg ataatgtgcc tgcgcgttgg ttctcaacgc tctcaatggg ggctctgttg
      481 gttct
//
LOCUS       DAJTOR010000408          481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTOR010000408 DAJTOR010000000
VERSION     DAJTOR010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.418"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(34..>481)
                     /locus_tag="OO249_004346"
     CDS             complement(34..>481)
                     /locus_tag="OO249_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR5638382.1"
                     /translation="SDYLKLQALFARVAEEKHRRGELEKLHHQLVDTYTSLNRQYAEL
                     LSEYKHLRRYFGVTVQVPYTDVWTHKPVQFYPGKHPCEKPAEMLQQIISASSRPGDLI
                     ADFFMGSGSTVKAALALGRRAIGVELETERFEQTVREVQDLVSQNG"
BASE COUNT          110 a          150 c          101 g          120 t
ORIGIN      
        1 gcataataac gacgcgtaat taattcttca atatcatccg ttctggctga ctaaatcctg
       61 tacttcccga accgtctgct caaaacgttc agtctccagc tcaacgccaa ttgcacgacg
      121 cccgagcgcc agtgccgctt tcactgtcga acccgacccc atgaaaaaat ctgcaatcag
      181 gtcacccgga cgactgctag cgctgattat ctgctgcagc atttctgccg gtttttcgca
      241 cggatgtttc ccgggataga actgcaccgg tttatgcgtc cacacatcgg tatacggcac
      301 ctgcaccgtc acgccaaaat accgccgcag atgtttatat tcactcagca gctccgcata
      361 ctgccggttc agtgaggtat acgtatccac cagctggtgg tggagctttt ccagttcacc
      421 acgccgatgc ttctcttctg ccacccgggc aaacagcgcc tgtaatttca gataatcgct
      481 t
//
LOCUS       DAJTOR010000409          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTOR010000409 DAJTOR010000000
VERSION     DAJTOR010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.419"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..332)
                     /locus_tag="OO249_004347"
     CDS             complement(<1..332)
                     /locus_tag="OO249_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5638383.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPE"
BASE COUNT          146 a          102 c          111 g          113 t
ORIGIN      
        1 ctttccggta aaaataaaaa cggtaaatag agcgcaatat cggaaataac atcgccagtt
       61 tcattcaaaa tcgcccccag acgtgtttgc tgattgcact cacgcgccaa catgccatcc
      121 agcgcattga gcgccatacg gataaaaagc acgatgggca atagcaaaaa gaggatgggt
      181 tgtgccacca acaccaacag caatccggta aaaagagaaa gcgccagtgc cgtaagagtg
      241 atgtgattcg ctgtaacgtg gtgcttataa agccaaaaca tcgtcggcct taacagcgac
      301 tgaaagagcg gttttatctg gtagagcgtc atttatcatt ccgtgatatt cagtttgctc
      361 tatggtgcat caattgtgat ggcggagata tcacaacgcg gagcatcaac gtaaattcgg
      421 aatatatcaa gatgtggtgg atggttttcc tacacatctg tttgattacc ga
//
LOCUS       DAJTOR010000410          448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTOR010000410 DAJTOR010000000
VERSION     DAJTOR010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.420"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(33..>448)
                     /locus_tag="OO249_004348"
     CDS             complement(33..>448)
                     /locus_tag="OO249_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5638384.1"
                     /translation="ANNSTKNAAFYLWGNPSRSVVAELGDDSGWHFFSQRNTDNSITF
                     AVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFRAPSGHGLSGI
                     AISDTGRDSADNVNGVYYRPLQKLINGTWYNVASV"
BASE COUNT          109 a          118 c           99 g          122 t
ORIGIN      
        1 cccgcagtaa tattttttaa tatgcaacat tgttaaacac tcgccacgtt ataccaggtg
       61 ccattaatca gtttctgaag cgggcggtaa tacacgccgt tgacgttatc cgctgagtca
      121 cggccggtat ccgatatcgc tatccctgac agcccgtgtc ccgaaggtgc gcgaaatctc
      181 cacgaaacgg tgttactgcc ggggctgtaa tacatttcat ggccataccg tacatcctgt
      241 accccctctg ttcgatattt atagcgggca tcgaagtttc cgtaatttaa cggaattacc
      301 tgtccgttta cggcgaacgt gatgctgtta tctgtattcc tctggctgaa aaaatgccag
      361 cctgaatcat cgccaagctc tgcaacaaca gacctggatg gattacccca caaataaaac
      421 gctgcatttt tcgtggagtt gttggcgc
//
LOCUS       DAJTOR010000411          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTOR010000411 DAJTOR010000000
VERSION     DAJTOR010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.421"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            35..447
                     /locus_tag="OO249_004349"
                     /pseudo
     CDS             35..447
                     /locus_tag="OO249_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          101 a          115 c          121 g          110 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacagtggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcac
//
LOCUS       DAJTOR010000412          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTOR010000412 DAJTOR010000000
VERSION     DAJTOR010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.422"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
BASE COUNT          171 a           70 c           79 g          114 t
ORIGIN      
        1 gactctatca tcgccaatca tcagacattg aaatagacga cactgagatg acgcttacaa
       61 ttcttttggt ttctgtaata agtaatctga catcccccgt gcccagaaga ggagagggat
      121 atcaagccca agggttaata attgatacta cggttaaaat tgccttcagt aaaggaaacg
      181 tgattaaatt caggggattt cagagtgaaa accatacaac atattaaaat ctgttcattt
      241 agctataata catacaagaa agtgtccagt tagataatgt tatctaaata accacagata
      301 aaaacgcaca taattggggg ggggatgaaa atacctgaag cagtaaatca tattaatgtt
      361 cagaataata ttgatcttgt tgatggaaaa acgaatccga ataaagcaac aaaagcatta
      421 caaaaaaata tatt
//
LOCUS       DAJTOR010000413          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTOR010000413 DAJTOR010000000
VERSION     DAJTOR010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.423"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>431
                     /locus_tag="OO249_004350"
     CDS             <1..>431
                     /locus_tag="OO249_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638385.1"
                     /translation="LKHERIKKETIRQKNAHILEHGWNATEFSWFRYGQYNETGIYIS
                     IEKTIIITITVSGGCFKKEYSIVSHMLVTDTITEATLYENGLYTRHIRLSRPVSDSKY
                     PLPPGSQLIKNMTLRLRLQDQQEETSVTLFQGKMSTDGNNY"
BASE COUNT          145 a          104 c           88 g           94 t
ORIGIN      
        1 gccttaaaca tgagcgaata aaaaaagaaa ctatcaggca aaaaaatgca catattctgg
       61 agcatggctg gaatgcaact gagttctcat ggttccgata cggacagtat aacgaaacgg
      121 gtatttacat ctcgatcgag aagaccatta tcattacaat caccgtttct ggtgggtgct
      181 ttaaaaaaga atacagcatt gtctcccaca tgctggtcac tgacaccata acagaggcca
      241 ccctgtacga aaacgggctg tatacgcgcc acatcagact cagtcgccca gtatctgaca
      301 gcaaataccc cttgccaccc ggatctcagc tcattaaaaa tatgacctta cgactgcgcc
      361 tgcaggatca gcaggaagaa acatccgtga cattatttca gggaaaaatg agtaccgacg
      421 gaaataacta t
//
LOCUS       DAJTOR010000414          425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTOR010000414 DAJTOR010000000
VERSION     DAJTOR010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.424"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..>425)
                     /locus_tag="OO249_004351"
     CDS             complement(<1..>425)
                     /locus_tag="OO249_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5638386.1"
                     /translation="LPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPEL
                     PTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRE
                     NRITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
BASE COUNT          107 a           74 c          118 g          126 t
ORIGIN      
        1 tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg
       61 atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtgtgt gatgcgattc
      121 tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct
      181 ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca
      241 gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca
      301 ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc aggcaattca
      361 ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca
      421 ggaag
//
LOCUS       DAJTOR010000415          423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTOR010000415 DAJTOR010000000
VERSION     DAJTOR010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.425"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            36..>423
                     /locus_tag="OO249_004352"
     CDS             36..>423
                     /locus_tag="OO249_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5638387.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLTAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQ"
BASE COUNT          105 a           99 c          129 g           90 t
ORIGIN      
        1 ggttcttcct gatttaattt atcgcgagaa aaactatgtc tgtagtgata tcaggtgcgc
       61 tgactgatgg cgcaggcatc cccatgtccg gatgccagat tattctgaaa tcccgtgtaa
      121 acacctcaga agtggtgatg cgtaccaggg ctgatgtagt gaccggaaat aacggtgaat
      181 attcgtttga ggcacaggtc ggaaaatatt gcgtgtatct gaaacgggac tggcgcgacg
      241 agtactgtgt tggcgacatt gctgtatacg acgactcaaa gcccggcacg ctgaatgact
      301 ttctgactgc ccccgatgaa ggcgacctga agccggatgt cgtcaaacgc tttgaggaaa
      361 tggtggcgca ggcgcagcag agcgccgggg ccgcagccgg aaacgcacag cagacggcgc
      421 agg
//
LOCUS       DAJTOR010000416          412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTOR010000416 DAJTOR010000000
VERSION     DAJTOR010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.426"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..363
                     /gene="actP"
                     /locus_tag="OO249_004353"
     CDS             <1..363
                     /gene="actP"
                     /locus_tag="OO249_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HCR5638388.1"
                     /translation="FMVGLAFAIAASCNFPIILLSMYWSKLTTRGTMLGGWLGLITAV
                     VLMILGPTIWVQILGHEKAIFPYEYPALFSISVAFLGIWFFSATDNSAEGARERELFR
                     AQFIRSQTGFGVEQGRAH"
BASE COUNT           69 a          125 c          111 g          107 t
ORIGIN      
        1 tttatggtag gccttgcgtt tgccatcgcg gcgagctgta acttcccgat cattctgctt
       61 tccatgtact ggtcgaaact gactacccgt ggcacgatgc tgggcggctg gctggggttg
      121 attaccgcag tggtgctgat gatcctcggc ccgactattt gggtacagat cctcggtcac
      181 gaaaaagcca tcttcccgta tgaatacccg gcgctgttct ctatcagcgt ggcattcctc
      241 ggcatctggt tcttctcggc aaccgataac tcagcggaag gtgcgcgcga gcgtgaactg
      301 ttccgtgcgc agtttatccg ctcccagacc ggctttggcg ttgagcaagg ccgcgcacat
      361 taatctctcc ccttccccgg tcgcctgacc ggggaatact cttcctctcc ag
//
LOCUS       DAJTOR010000417          410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTOR010000417 DAJTOR010000000
VERSION     DAJTOR010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.427"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..309)
                     /locus_tag="OO249_004354"
     rRNA            complement(<1..309)
                     /locus_tag="OO249_004354"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a          114 c           84 g          125 t
ORIGIN      
        1 tccagacgct tccactaaca cacacactga ttcaggctct gggctgctcc ccgttcgctc
       61 gccgctactg ggggaatctc ggttgatttc ttttcctcgg ggtacttaga tgtttcagtt
      121 cccccggttc gcctcattaa cctatggatt cagttaatga tagtgtgacg aatcacactg
      181 ggtttcccca ttcggaaatc gccggttata atggttcata tcaccttacc gacgcttatc
      241 gcagattagc acgtccttca tcgcctctga ctgccagggc atccaccgtg tacgcttagt
      301 cgcttaacct cacaacccga agatgtttct tacgattcat catcgtgttg cgaaaatttg
      361 agagactcac gaacaacttt cgttgttcag tgtttcaatt ttcagcttga
//
LOCUS       DAJTOR010000418          409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTOR010000418 DAJTOR010000000
VERSION     DAJTOR010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.428"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>409
                     /locus_tag="OO249_004355"
     CDS             <1..>409
                     /locus_tag="OO249_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5638389.1"
                     /translation="ASATTSHISFSYAARQRMQNRARLLKQYQTHLKKQASYIVEGNA
                     ESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALVENNHFLSSNHLHNITEVAIHRL
                     EQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKIL"
BASE COUNT          114 a          118 c          101 g           76 t
ORIGIN      
        1 tcgccagcgc caccacatca catatctctt tcagctacgc cgcccgccag cggatgcaaa
       61 accgtgcgcg tttattaaaa cagtaccaaa cccatctgaa aaaacaggcc agctacattg
      121 tggaaggcaa tgccgaaagc agaagggcgc tacgccagca caaccgggag cagataaaac
      181 agcatccaga atggtttcct gcgccgctca aggcaagtga cagacgctgg caggcgttgg
      241 tagaaaacaa tcatttcctc agcagcaacc acctgcataa tatcaccgaa gtggcgattc
      301 accgtctgga gcaacagctt ggcaagcctt acgtctgggg cggcacgcgg cccgatcaag
      361 gcttcgactg tagcgggttg gttttttatg cctacaacaa gatccttga
//
LOCUS       DAJTOR010000419          405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTOR010000419 DAJTOR010000000
VERSION     DAJTOR010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.429"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>405
                     /locus_tag="OO249_004356"
     CDS             <1..>405
                     /locus_tag="OO249_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5638390.1"
                     /translation="TRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFR
                     ATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVG
                     YAMGERMTKELTGKALFMALRSQRPPAGLIHHS"
BASE COUNT          104 a          113 c          113 g           75 t
ORIGIN      
        1 cactcgcgaa acatatggaa cccggcggct ccagacggag ctggcagaga atggcatcat
       61 cgttggtcgt gaccgactgg cacgtcttcg taaggagcta aggctacgct gtaagcagaa
      121 acgcaagttc agagcgacta cgaacccgaa ccacaatctg ccagttgcgc caaatctgct
      181 gaaccagacg ttcgctccta cagcaccaaa tcaggtctgg gtggcggacc tgacgtatgt
      241 tgccacacag gagggatggt tgtacctcgc tggcatcaaa gatgtttata cgtgcgaaat
      301 tgtcggctac gccatgggag agcgcatgac aaaagagctg acaggtaaag ccctgtttat
      361 ggcgctcagg agccagcgcc cacctgccgg gctaatccac cactc
//
LOCUS       DAJTOR010000420          402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTOR010000420 DAJTOR010000000
VERSION     DAJTOR010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.430"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>402
                     /locus_tag="OO249_004357"
     CDS             <1..>402
                     /locus_tag="OO249_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCR5638391.1"
                     /translation="QIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQ
                     HFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELT
                     SALIVNPYDRDEVAAALDRALTMSLAERISR"
BASE COUNT           97 a           93 c          107 g          105 t
ORIGIN      
        1 cccagattgc accaacgtcg cgtggtgatg tgcaagccta tcaggatatt cgtcatcagc
       61 tcgaaaatga agctggacga attaatggta aatacgggca attaggctgg acgccgcttt
      121 attatttgaa tcagcatttt gaccgtaaat tactgatgaa aatattccgc tactctgacg
      181 tgggcttagt gacgccactg cgtgacggga tgaacctggt agcaaaagag tatgttgctg
      241 ctcaggaccc agccaacccg ggcgttcttg ttctttcgca atttgcggga gcggcaaacg
      301 agttaacgtc ggcgttaatt gttaacccct acgatcgtga cgaagttgca gctgcgctgg
      361 atcgtgcatt gactatgtcg ctggcggaac gtatttcccg tc
//
LOCUS       DAJTOR010000421          401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJTOR010000421 DAJTOR010000000
VERSION     DAJTOR010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.431"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..250
                     /locus_tag="OO249_004358"
     CDS             <1..250
                     /locus_tag="OO249_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; involved in N,N'-diacetylchitobiose
                     transport; protein IIA transfers a phosphoryl group to IIB
                     which then transfers the phosphoryl group to the sugar;
                     IIC forms the translocation channel for the sugar uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR5638392.1"
                     /translation="ITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNTNGSVAALLVALF
                     NLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            301..>401
                     /locus_tag="OO249_004359"
     CDS             301..>401
                     /locus_tag="OO249_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; involved N,N'-diacetylchitobiose transport;
                     protein IIA transfers a phosphoryl group to IIB which then
                     transfers the phosphoryl group to the sugar; IIC forms the
                     translocation channel for the sugar uptake; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5638393.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSL"
BASE COUNT          111 a           96 c          102 g           92 t
ORIGIN      
        1 aatcaccctc gcagcgtact acatgggcat tattcctccg gtgaccaata ttgcaccgtg
       61 gaccatgcca accggtctgg gagccttctt taacaccaac ggtagcgtcg ctgcattgct
      121 ggtcgcactc ttcaaccttg gtatcgcaac gttaatttat ctgccctttg ttgtggtggc
      181 taacaaagcg caaaacgcga ttgataaaga agagagcgaa gaagatatcg ctaacgccct
      241 gaaattttaa tcgatgccgg tacggttaat cgtgccggta agaagagaga ggaacgatgt
      301 atgatggatc tcgataacat tcccgatacg caaacagaag ctgaagagct ggaagaagtg
      361 gtgatggggc ttatcatcaa ctccggacaa gcgcgcagcc t
//
LOCUS       DAJTOR010000422          400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTOR010000422 DAJTOR010000000
VERSION     DAJTOR010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.432"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..224)
                     /locus_tag="OO249_004360"
     CDS             complement(<1..224)
                     /locus_tag="OO249_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCR5638394.1"
                     /translation="MTWNLLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLR"
BASE COUNT          134 a           73 c           65 g          128 t
ORIGIN      
        1 tttcttaata agaaggtatt aaattcatct ggattgctga tactacttac cggacaaata
       61 aaggtttcga taatcatttg gcgggaagta aaaagaatat tgatttgcaa atcgccataa
      121 tcattcattt taataattaa tgcgttctcg ctatttgtta catcaatatt ttgttcaggt
      181 acagtttgca gcgccgtcgc cagcgccaac agattccatg tcatatatta ttccatatag
      241 atttagttta aatattaata aaatgaatat ttgcagtacg taattatctt accagctata
      301 gacaaaaaaa aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc
      361 tgacgcaccg cttcaaataa gcaaattccg gttgcaaccg
//
LOCUS       DAJTOR010000423          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTOR010000423 DAJTOR010000000
VERSION     DAJTOR010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.433"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..397
                     /locus_tag="OO249_004361"
                     /pseudo
     CDS             1..397
                     /locus_tag="OO249_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
BASE COUNT          105 a           82 c           99 g          111 t
ORIGIN      
        1 ttggtaaaga aatcgccatt acattcgcga cagctggcgc atctgtggtg gtcagtgata
       61 ttaacgccga cgcagctaac catgttgtag acgaaattca acaactgggg gggcaggcat
      121 ttgcctgccg ttgtgatatt acttccgaac aggaactctc tgcactggca gatttgctat
      181 cagtaagctg ggtaaagttg atattctggt taacaacgcc ggtggcggtg gacctaaacc
      241 gtttgatatg ccaatggcgg attttcgccg tgcttatgaa ctgaatgtgt tttctttttt
      301 ccatctgtca caacttgttg cgccagaaat ggaaaaaaat ggcggtggcg ttattctgac
      361 catcacttct atggcggcag aaaataaaaa tataaac
//
LOCUS       DAJTOR010000424          396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTOR010000424 DAJTOR010000000
VERSION     DAJTOR010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.434"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..396
                     /locus_tag="OO249_004362"
                     /pseudo
     CDS             1..396
                     /locus_tag="OO249_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane; internal
                     stop; incomplete; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
BASE COUNT          105 a           95 c          103 g           93 t
ORIGIN      
        1 attcaggacg aaaccgggca atttaaaccc tacccggcaa gtaacttctc cactgctgtt
       61 ccgcaaagcg ccacggcaat gctggtcacg gcattgaaag attctcgctg gtttataccg
      121 ctggagcgct agggcttaca aaacctgctt aacgagcgca agattattcg tgcggcacaa
      181 gaaaacggca cggttgccat taataaccga atcccgctgc aatctttaac ggcggcaaat
      241 attatggttg aaggttcgat tatcggttat gaaagcaacg ttaaatcggg cggtgttggg
      301 gcaagatatt ttgggatcgg tgcagacacg caataccagc tcgatcagat tgccgtgaac
      361 ctgcgtgtcg tcaatgtgag taccggtgag atcctc
//
LOCUS       DAJTOR010000425          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTOR010000425 DAJTOR010000000
VERSION     DAJTOR010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.435"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            107..385
                     /locus_tag="OO249_004363"
                     /pseudo
     CDS             107..385
                     /locus_tag="OO249_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          104 a           94 c           86 g          101 t
ORIGIN      
        1 tttaacgtaa ggtgagagga tttttaagtg tatccataga ttaaaaatga caatgcctcc
       61 aattagttga attaggggtt ataatacccg cttttaaggt tctttcatgg tcacagttaa
      121 ctttcattgc ccccgttgcc agtccgttca ggtttaccgt cacgggcaga acccaaaagg
      181 acatgatcga ttccgttgcc gtgactgtca ccgggtgttt cagctcactt actgttacga
      241 ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg gcttcaacgg tgccggagtt
      301 cgcgatactg cccgtacgct gaaaatcggc atcaataccg tcatacggac attaaaaagc
      361 tcccgccccg gcggataaca tctgc
//
LOCUS       DAJTOR010000426          381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTOR010000426 DAJTOR010000000
VERSION     DAJTOR010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.436"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            183..338
                     /locus_tag="OO249_004364"
                     /pseudo
     CDS             183..338
                     /locus_tag="OO249_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          120 a           83 c           96 g           82 t
ORIGIN      
        1 agagcagaac gggatctata cttttgcggg tcaacataag aggatgagtc cttaataaaa
       61 caggtcctca gagaatacat attctgcgga gcgtgcggca acaacaggac gaacaggggc
      121 ggttttcaat ttccagccgt caggctgccg tggttcactc caacaacgga gacaggtaaa
      181 ttatggacga aaagaagctt aaagcccttg cggctgaact ggctaaaggc ctcaaaaccg
      241 aggccgacct caatcagttt tcccgtatgc agacgaaatt aactgtcgaa acggtgctta
      301 atgaggaact aactgaccat ctcgggcatg aaaaaagcta catccggtag taaacattat
      361 cagtgggtta tgcgatatga t
//
LOCUS       DAJTOR010000427          377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTOR010000427 DAJTOR010000000
VERSION     DAJTOR010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.437"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(100..>377)
                     /locus_tag="OO249_004365"
     CDS             complement(100..>377)
                     /locus_tag="OO249_004365"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5638395.1"
                     /translation="HALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPS
                     LKDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
BASE COUNT           92 a          103 c           98 g           84 t
ORIGIN      
        1 aaagcagaaa aaacgccgca gagtgcggcg tttgggattg tttgctattt cttgccggat
       61 gcgacgtaaa tgcctcaccc ggcctggtag aacttaaaat tactcgccta ctttcgccca
      121 ggtatcacgc agcccaacgg tgcggttaaa taccggtttt tccgctgtgg aatggcggct
      181 gtcgaggcag aagtaacctt cacgctcaaa ctggaatgct ttacccgcaa ccgcatcttt
      241 cagcgacggt tcagcaaagc cctgtttgat caccagcgat tccgggttaa tcaccgacag
      301 gaaatcatcc gcagcacctg ggttaggcac gctgaacaga cggtcgtaca gacggatttc
      361 aaccggcagc gcatgtg
//
LOCUS       DAJTOR010000428          376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTOR010000428 DAJTOR010000000
VERSION     DAJTOR010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.438"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..247)
                     /locus_tag="OO249_004366"
     CDS             complement(<1..247)
                     /locus_tag="OO249_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638396.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPKTFAVAMREL"
BASE COUNT           95 a           88 c          102 g           91 t
ORIGIN      
        1 ctaattcacg catcgccacg gcgaacgttt tcgggcgcgg cagcatgtgc ggaagcgggg
       61 aagtgtgatc agaaagcgct tcgatatcaa aaaacactgc gccgggaata tgtccattca
      121 gatactcctg agcaacgtta cgatcttcct gtccaggcga cgccatgcgg gcatcgataa
      181 tctgaatttc cgggtcatca atatgttcgg cgagccagtc ggctcctaca aaccatgtcg
      241 tggacatagg catctccatt tttgccgggg ttaggctaaa ttgtcggatg tttagtgaaa
      301 agcgacaagc gaaagaggct gatttggctg tctgagccac aattctcact aaaattcgga
      361 ttgtatgggc gtgata
//
LOCUS       DAJTOR010000429          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTOR010000429 DAJTOR010000000
VERSION     DAJTOR010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.440"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(189..296)
                     /locus_tag="OO249_004367"
     CDS             complement(189..296)
                     /locus_tag="OO249_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5638397.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWHNRK"
BASE COUNT           81 a           96 c           92 g          101 t
ORIGIN      
        1 aagagtgcat tccggttttt caacagctgt tacagtcgtt tcatgagtgc tctggatgat
       61 gcttccagct cgggttgcca atatttactt gtggaagaga taaagacaaa aatggccgca
      121 ggctgttacc cctgcggccg gtttcgggcg catattgcca tcacggcagc ctgacgcccg
      181 ttttcacctt acttccggtt atgccaccag ccgacaatcg ctgcggtaat aatccccgcc
      241 aggatcggtg ctgccaggtc gtgccagaaa atcatggcaa acttcgcgag cgtcatatag
      301 ccgccttgtt gtaatgacaa cattttgcgg ctattcttga attgttgatg gttcaagatt
      361 agcccccgtt
//
LOCUS       DAJTOR010000430          368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTOR010000430 DAJTOR010000000
VERSION     DAJTOR010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.441"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..358)
                     /locus_tag="OO249_004368"
     CDS             complement(<1..358)
                     /locus_tag="OO249_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5638398.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKQARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQ"
BASE COUNT           89 a           83 c           90 g          106 t
ORIGIN      
        1 tctgcttaat tttgctttca catttttgta agtcttctgc cggattgtgc gacgtctgca
       61 atatcacgtc gaaaccttcc tcttcagctt tggcggtgat ggcatgcaaa acttcagaga
      121 aaaacggatt acccgccgta gttttggtcg aacgggtaga aatcaccata atggcatcaa
      181 aacccgaaga ggtcagcgcg cgggcctgct tgtttggctg atactgtaat tcttcaatgg
      241 cccgtaacac tttttcgcgc gcttccgggg agatattagt ttgcttattc agcacgcgtg
      301 atacggtgga ttttgatacg cctgcaaccc tggcaatatc ataaatagta ggggacatag
      361 gtcaggaa
//
LOCUS       DAJTOR010000431          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJTOR010000431 DAJTOR010000000
VERSION     DAJTOR010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.442"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            31..364
                     /locus_tag="OO249_004369"
                     /pseudo
     CDS             31..364
                     /locus_tag="OO249_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
BASE COUNT          106 a           73 c           91 g           94 t
ORIGIN      
        1 gttgttaata aaaaccctac aggaggcact atgcctaaaa aaatgagaac aacccgtaat
       61 ctattattaa tggctacgct attaggtagc gcactatttg ccagggctgc ggataaagaa
      121 atgaccattg gtgcaatata ccttgatacc tagggatatt acgctggagt gcgtcagggc
      181 gttcaggatg cggcaaaaga ttcttcagta caggtacagt taattgaaac taacgcccag
      241 ggtgatattt cgaaagaaag tacctttgtt gataccctcg tggcgcgtaa tgtcgatgcc
      301 attattttat cggcagtgtc tgaaaatggc agtagccgta ccgttcgtcg cgccagtgaa
      361 gcgg
//
LOCUS       DAJTOR010000432          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJTOR010000432 DAJTOR010000000
VERSION     DAJTOR010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.443"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            84..>364
                     /locus_tag="OO249_004370"
     CDS             84..>364
                     /locus_tag="OO249_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5638399.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRRP"
BASE COUNT           81 a          104 c           85 g           94 t
ORIGIN      
        1 taaccggctc atttaaaccg tctggtctgt ttcctccggc tccacaaaaa taatgtccat
       61 cattttgaag tggacactat cgtatggaat accggacctg gatcactgaa gctttacgcc
      121 ttcatttcga agaacattta ccacgggttg tggccggacg tcgcctgggc gtaccaaaat
      181 caactgtctg tggtatgttc gtgcgcttcc gcaacgctgg cctttcatgg cctctgcccg
      241 caggtatgtc ggagcaggaa cttgatgccc ttctttacgg aagtgcctcc acagtacctg
      301 tcgtacttac tgaaagcacc gttatgccca aactcccggt ggtaaaaaaa cgtccccggc
      361 ggcc
//
LOCUS       DAJTOR010000433          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJTOR010000433 DAJTOR010000000
VERSION     DAJTOR010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.444"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>361
                     /locus_tag="OO249_004371"
     CDS             <1..>361
                     /locus_tag="OO249_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5638400.1"
                     /translation="KQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHL
                     KGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPR
                     VVKERPWKYPTAPKKSQS"
BASE COUNT          108 a           71 c          111 g           71 t
ORIGIN      
        1 aaacagacga tgcaactgag caggctgacg ctgagaagta aaaagccgga gcttgtggag
       61 caagagctgt ggggtgtctt actggcttat aatctggtga gatatcagat gattaaaatg
      121 gcggaacatc tgaaaggtta ctggccgaat caactgagtt tctcagaatc atgcggaatg
      181 gtgatgagaa tgctgatgac attgcagggc gcttcaccgg gacgtatacc ggagctgatg
      241 cgcgatcttg caagtatggg acaacttgtg aaattaccga cgagaagggg aagggccttc
      301 ccgagagtgg taaaggagag gccctggaaa taccccacag ccccgaaaaa gagccagtca
      361 g
//
LOCUS       DAJTOR010000434          355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJTOR010000434 DAJTOR010000000
VERSION     DAJTOR010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.445"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>355
                     /gene="ospC4"
                     /locus_tag="OO249_004372"
     CDS             <1..>355
                     /gene="ospC4"
                     /locus_tag="OO249_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5638401.1"
                     /translation="RKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSLEHLDK
                     VSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFDKIQSE
                     NIQTHKNTYSEDIKEI"
BASE COUNT          126 a           68 c           48 g          113 t
ORIGIN      
        1 aaggaagtct atagcagcgc aaagttatag caaaatgttt tctcaaggga ccagtttcaa
       61 atcactaaat ctttctattg aagcgccatc tggagcacgg tcatcatttc ggtcacttga
      121 gcatctcgat aaggtgtcca ggcactatat ctctgagata atacaaaaag ttcatcctct
      181 ttcttcagac gaacgtcact tactttctat tattattaat tctaatttta acttcagaca
      241 tcaaagtaac tctaatctgt caaataatat tcttaatatt aaatcctttg ataaaattca
      301 gtctgaaaat atacaaacac ataagaatac atactctgag gatattaaag aaatc
//
LOCUS       DAJTOR010000435          354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJTOR010000435 DAJTOR010000000
VERSION     DAJTOR010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.446"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>354
                     /locus_tag="OO249_004373"
     CDS             <1..>354
                     /locus_tag="OO249_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR5638402.1"
                     /translation="KAKNDKRVDAIEQEKGKLAGQVETLNGKLSELENLKSDLEKELL
                     ELKRPAGGAQNKLATEHKEAFVGFLRKGRGDGLRDLERKALQVGTDEDGGYAVPEALD
                     RNILTLLKDEVVMRQ"
BASE COUNT          103 a           80 c          115 g           56 t
ORIGIN      
        1 caaagcaaag aacgacaagc gcgtggatgc gattgagcag gaaaaaggca agcttgccgg
       61 gcaggtggaa accctgaacg ggaaactcag cgagctggaa aacctcaaaa gcgatcttga
      121 aaaagagctg cttgagctga aacgtccggc aggtggtgcg caaaataaac tggccaccga
      181 gcataaagaa gcgtttgtgg gcttcctgcg taaaggccgt ggagatggtc tgcgcgatct
      241 ggagcgcaag gcattacagg tgggcaccga tgaagacggc ggctatgccg tgccggaagc
      301 actggatcgc aacattctca ccctgctgaa agatgaagtg gtgatgcgcc agga
//
LOCUS       DAJTOR010000436          354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJTOR010000436 DAJTOR010000000
VERSION     DAJTOR010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.447"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..>354)
                     /locus_tag="OO249_004374"
     CDS             complement(<1..>354)
                     /locus_tag="OO249_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="HCR5638403.1"
                     /translation="IVCAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMA
                     TLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARL
                     GRKSLSFSKSVELHD"
BASE COUNT           74 a          116 c           85 g           79 t
ORIGIN      
        1 ttgtcatgca gctccaccga ttttgagaac gacagcgact tccgtcccag ccgtgccagg
       61 tgctgcctca gattcaggtt atgccgctca attcgctgcg tatatcgctt gctgattacg
      121 tgcagctttc ccttcaggcg ggattcatac agcggccagc catccgtcat ccatatcacc
      181 acgtcaaagg gtgacagcag gctcataaga cgccccagcg tcgccatagt gcgttcaccg
      241 aatacgtgcg caacaaccgt cttccggagc ctgtcatacg cgtaaaacag ccagcgctgg
      301 cgcgatttag ccccgacgta tccccactgt tcgtccattt ccgcgcagac gatg
//
LOCUS       DAJTOR010000437          351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJTOR010000437 DAJTOR010000000
VERSION     DAJTOR010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.448"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..274
                     /locus_tag="OO249_004375"
     CDS             <1..274
                     /locus_tag="OO249_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5638404.1"
                     /translation="LITSIGAIEHDGTLLEFDLNEALVARTMIKHARNTLLVADHTKF
                     AASAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
BASE COUNT           96 a           72 c           84 g           99 t
ORIGIN      
        1 tttaatcacc agtataggtg ccattgaaca tgacggcacc ctactggaat ttgatctaaa
       61 tgaagcgtta gtcgcgagaa cgatgattaa acatgcgcgg aatacattgt tagtggccga
      121 tcatacgaag tttgccgcgt ctgctgccgt ttcaattggc aatgcacgga atgtcagggc
      181 tttttttact gatgccccgc ctcccaattc tttctgccag ttgttaagtg aagagaatgt
      241 tgaactggtg gttgccgagc aagaagtatc ctgaaattga aatttcggaa aatccgctgg
      301 gaatttcagc ggattttgtt ttccataagc aaaaaaaacc tgccagcgat g
//
LOCUS       DAJTOR010000438          350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJTOR010000438 DAJTOR010000000
VERSION     DAJTOR010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.449"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..>350)
                     /locus_tag="OO249_004376"
     CDS             complement(<1..>350)
                     /locus_tag="OO249_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5638405.1"
                     /translation="TSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSVLPWVTGL
                     TLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGSLYGLMPL
                     YLNHKGVSNASIGF"
BASE COUNT          101 a           99 c           76 g           74 t
ORIGIN      
        1 gaaaccaatg ctggcattgc tcaccccttt gtgattgagg tacagcggca tcaggccata
       61 tagagatccc agaacgattc ctgagataat gcagccattc acgccaagcc gcgcctgacg
      121 gagttttagc attgacgtaa ttgacgtcga atcatggttt tcagcctgct gattcagcac
      181 acgcgtaaac aacagcggta agatccctgc caacgtcaaa cctgtaaccc acggcaatac
      241 ggacatcagc tcggttgaaa ctttgctgac cagtaactgg cctaaaaacg ttcccacgta
      301 ataaaccatc atatacgcag caagcaaacg cccacggtta cgtgacgtcc
//
LOCUS       DAJTOR010000439          347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJTOR010000439 DAJTOR010000000
VERSION     DAJTOR010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.450"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            1..347
                     /locus_tag="OO249_004377"
                     /pseudo
     CDS             1..347
                     /locus_tag="OO249_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           81 a           84 c          104 g           78 t
ORIGIN      
        1 gatggaaaat gaactcctca aagaagccgt tgaatatgga cgggcaaaaa agtggatagc
       61 gcacgcgccc ttattgcccg gggatgggga gtaagcttag tcagccgttg tctccgggtg
      121 tcgcgtgcgc agttgcacgt cattctcaga cgaaccgatg actggatgga tggccgccgc
      181 agtcgtcaca ctgatgatac ggatgtgctt ctccgtatac accatgttat cggagagctg
      241 cccacgtatg gttatcgtcg ggtatgggcg ctgcttcgca gacaggcaga acttgatggt
      301 atgcctgcga tcaatgccaa acgtgtttac cggatcatgc gccagaa
//
LOCUS       DAJTOR010000440          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJTOR010000440 DAJTOR010000000
VERSION     DAJTOR010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.451"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(85..340)
                     /locus_tag="OO249_004378"
                     /pseudo
     CDS             complement(85..340)
                     /locus_tag="OO249_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT           85 a           89 c           73 g           93 t
ORIGIN      
        1 ccgcgcctta tatccccgcc ctgaaggatg gggttttacg gcgcaccgga taaaaagcgc
       61 catctaacga tggcgcgtct ggaactactt tttgttaacg tcaaacatgg tggcatcggt
      121 cgccatgtca tcaataactt gtttaatatt ttcgaaagcc attggcgtac tttcgttatt
      181 caggtcttta ccttagccct gacgaacgac tttgataacc ggtttattag tcgctgcatc
      241 aatcagctca ccttcaaaat agaggcgagt atccatggta cggtgacctg tagccatttg
      301 cgtccccgca accactaatg caacaggaac cacttcatag
//
LOCUS       DAJTOR010000441          337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJTOR010000441 DAJTOR010000000
VERSION     DAJTOR010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.452"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(149..>337)
                     /gene="csgC"
                     /locus_tag="OO249_004379"
     CDS             complement(149..>337)
                     /gene="csgC"
                     /locus_tag="OO249_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5638406.1"
                     /translation="GGSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVS
                     DGQALHLSQQWPPSSEKS"
BASE COUNT           92 a           71 c           66 g          108 t
ORIGIN      
        1 taagagagtt taaaaaaggt ctgaacattt attctatttc ctcaatgatt agtcatcctt
       61 gagggttgtg ttatccatac tttcaggttg gctgattcgc agcagaccat tctctccaga
      121 ttcatcttat gctcgacatt tcaacaaatt aagacttttc tgaagagggc ggccattgtt
      181 gtgataaatg aagtgcctgt ccatcagaaa cagtaacaac tattttcacc cgatcttccg
      241 gggaaatatt taaactcaac ttcgtcaaag caataggttg attagcaggc aatgagaggg
      301 tcttttcttg cttcgtctga ctttgccctg aaccgcc
//
LOCUS       DAJTOR010000442          337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJTOR010000442 DAJTOR010000000
VERSION     DAJTOR010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.453"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(1..58)
                     /locus_tag="OO249_004380"
     tRNA            complement(1..58)
                     /locus_tag="OO249_004380"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31..33),aa:Thr,seq:tgt)
     gene            complement(69..145)
                     /locus_tag="OO249_004381"
     tRNA            complement(69..145)
                     /locus_tag="OO249_004381"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(109..111),aa:Arg,seq:tct)
     gene            complement(147..223)
                     /locus_tag="OO249_004382"
     tRNA            complement(147..223)
                     /locus_tag="OO249_004382"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(187..189),aa:Ile,seq:cat)
BASE COUNT           83 a           92 c           84 g           78 t
ORIGIN      
        1 taccggaatc gaactggtga cctactgatt acaagtcagt tgctctgcct gctgagctaa
       61 gtcggcgctg gcccaccacc gaggactcga acctcgcacc gtcaacttag aaggttgatg
      121 ctctatccgg atgagctagt ggtggttggt ggcccttgct ggacttgaac cagcgacctg
      181 gcgattatga gtcgctcgct ctgaccaact gagctaaagg gccggaggca gaataataac
      241 catatgtcat cacatctgca aactcatctg accaccagca cgtttaacgt cctgtgccgt
      301 ttttcaggta taaaaaaacc cgcataaagc gggctct
//
LOCUS       DAJTOR010000443          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJTOR010000443 DAJTOR010000000
VERSION     DAJTOR010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.454"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..>333)
                     /locus_tag="OO249_004383"
     CDS             complement(<1..>333)
                     /locus_tag="OO249_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="activates the production of the major fimbrae
                     protein FimA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbriae biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCR5638407.1"
                     /translation="VKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHAVQMIL
                     SGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNK
                     TVSAHKSN"
BASE COUNT          105 a           64 c           56 g          108 t
ORIGIN      
        1 tattagattt atgcgcacta actgttttat tgctaagcaa taacttatcg gcaatttctt
       61 tattagataa tccgctaacc agataacgta atatggtcac ttcacgatta gatagcacag
      121 tgaccgttga actattcgta ctacatttat tgctttttat atagttaagc gtttcgctgg
      181 gaaaaaacgt gtatccggag aggatcatct gaacggcatg aaaaatatca ttctgatcat
      241 tgcatttact gacaaaaccg ttagcaccag cttgtatcgc tctgccagca taaaagcatt
      301 ctgatttcga tgataaaaat aacactttca ctg
//
LOCUS       DAJTOR010000444          326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJTOR010000444 DAJTOR010000000
VERSION     DAJTOR010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.455"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>326
                     /locus_tag="OO249_004384"
     CDS             <1..>326
                     /locus_tag="OO249_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5638408.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEA"
BASE COUNT           74 a           85 c           97 g           70 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagc
//
LOCUS       DAJTOR010000445          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJTOR010000445 DAJTOR010000000
VERSION     DAJTOR010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.456"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(148..>323)
                     /locus_tag="OO249_004385"
     CDS             complement(148..>323)
                     /locus_tag="OO249_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5638409.1"
                     /translation="LLLSYTVGSLLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLL
                     RNAGHTPKPVAHV"
BASE COUNT          122 a           58 c           71 g           72 t
ORIGIN      
        1 aagaagtgcg gaaatagcga aatgcaaaat tataacctgc tgcacatttt atttttaaac
       61 gaataaaatt tggaataaag caagtcagat agaagaaaca aaaaagcaga gtgtgaaaca
      121 gtaagttcac actctgcttg aattcattta cacgtgagca acgggtttcg gcgtatgacc
      181 ggcgttgcgc agcagcatca gcaaatagat aaacgatacg ctggcgatca tgataaacaa
      241 taaattatcg gagaaattct gcattagcat agcggtaaat gacgggccaa gcagacttcc
      301 cacagtatag ctcaacagta agg
//
LOCUS       DAJTOR010000446          319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJTOR010000446 DAJTOR010000000
VERSION     DAJTOR010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.457"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..>319)
                     /locus_tag="OO249_004386"
     CDS             complement(<1..>319)
                     /locus_tag="OO249_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR5638410.1"
                     /translation="QQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVYIV
                     FVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVVVQ
                     AMGL"
BASE COUNT           87 a           88 c           83 g           61 t
ORIGIN      
        1 gcagccccat tgcctggaca accacaaaag cgaaaaaggc gttaagcccc atcgcaggag
       61 ccagcgccac cggaaggttt gcaaacaatc ccatcataat gctaccgaaa gcagcaatca
      121 gacaggttgt tacgaagacg gcactggtgt ccatgccagc aacgcccaga atttgtgggt
      181 taacaaaaac gatgtaaacc atcgtcagga aggtggtaaa accggcgatc acttcggtcc
      241 gtgccgtcgt gccatgttcg cgcagtttaa acacgcgttc cagcatcccc tggccagaag
      301 cctgggttgt gtgttgttg
//
LOCUS       DAJTOR010000447          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJTOR010000447 DAJTOR010000000
VERSION     DAJTOR010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.458"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..268)
                     /locus_tag="OO249_004387"
     CDS             complement(<1..268)
                     /locus_tag="OO249_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5638411.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGN"
BASE COUNT           89 a           75 c           85 g           63 t
ORIGIN      
        1 gattgcccgg cagcatgatg tccaggcgat tcacaatctg gtgaagaggt tctttacgct
       61 caagcgccat gccaacaata caccagacca tcatttcgag gggaagacgg cgcttgcgta
      121 gcgttacagt acctgattcg gcaaggcaac gagagatgag ttcggggtcg aggtaatccc
      181 ccagagaagt cagtgggtta cgcagagaat cgtaacggga taccagatca agagcctgtc
      241 caatgtgcat aaaaaaatcc ggaaacaagt gagcgtttcc ggattcttac acagccactg
      301 gatcggtcaa ct
//
LOCUS       DAJTOR010000448          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJTOR010000448 DAJTOR010000000
VERSION     DAJTOR010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.459"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            95..>310
                     /locus_tag="OO249_004388"
     CDS             95..>310
                     /locus_tag="OO249_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638412.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAAQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVL"
BASE COUNT           99 a           66 c           65 g           80 t
ORIGIN      
        1 tatttcttgc agtaattata aaagccgaaa acagaactca aaaacaatct ggctaaataa
       61 aagtaacaaa atttgcttta aggaagaatt ttctatgtac acacagaccc tgtatgagtt
      121 aagtcaggag gctgaacgcc tgttacagct ttctcgccaa cagttgcagt tactggaaaa
      181 aatgcctctc tctgtacccg gagacgatgc ggcacaactg gctttaccct ggagtcagcc
      241 taatatcgcc gaacgttacg cgatgctgaa taatgagttg cgtaaaattt cccgactgga
      301 aatggtgctg
//
LOCUS       DAJTOR010000449          307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJTOR010000449 DAJTOR010000000
VERSION     DAJTOR010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.460"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>307
                     /locus_tag="OO249_004389"
     CDS             <1..>307
                     /locus_tag="OO249_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5638413.1"
                     /translation="WKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATQRGY
                     QMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNHLERQFAVTEPNQVWCGDVT
                     "
BASE COUNT           77 a           81 c           94 g           55 t
ORIGIN      
        1 tggaaaaacc gtcctgaaaa accagacggc agacgggctg tattacgcag ccaggtactt
       61 gaactgcatg gcatcagcca cggctctgcc ggagcaagaa gcatcgccac aatggcaacc
      121 cagagaggct accagatggg gcgctggctt gctggcagac tcatgaaaga gctggggctg
      181 gtcagttgcc agcagccgac tcaccggtat aagcgtggcg gtcatgagca cgttgctatc
      241 ccgaatcatc ttgagcgaca gttcgccgta acggaaccaa atcaggtgtg gtgcggtgat
      301 gtgacct
//
LOCUS       DAJTOR010000450          306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.462, whole genome shotgun sequence.
ACCESSION   DAJTOR010000450 DAJTOR010000000
VERSION     DAJTOR010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.462"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..>306)
                     /locus_tag="OO249_004390"
     CDS             complement(<1..>306)
                     /locus_tag="OO249_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR5638414.1"
                     /translation="GITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCVLTLSLLVLL
                     IFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFISTKML"
BASE COUNT          126 a           73 c           46 g           61 t
ORIGIN      
        1 gtagcatctt tgtcgagata aagaaaacag tccccatccc aatggccatt aaaatcggca
       61 agaaaaaggt tacccaccag aaccctttca tagagcaatt aaaactaaat cttacgccgt
      121 tgagactagt catcaaggcc tgataacgca gtccttttgc tgccataaat ataagcaaaa
      181 ccaacagtga caaagtcaaa acacagccaa caagcggcat atctgctgac actgtcatta
      241 agattgcgaa atagaaaaaa acaaaaacaa gacaactaaa aaaaacattc ccaccagtaa
      301 ttccat
//
LOCUS       DAJTOR010000451          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJTOR010000451 DAJTOR010000000
VERSION     DAJTOR010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.denovo.464"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..>304
                     /locus_tag="OO249_004391"
     CDS             <1..>304
                     /locus_tag="OO249_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5638415.1"
                     /translation="REGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRV
                     NRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDA"
BASE COUNT           59 a           72 c           99 g           74 t
ORIGIN      
        1 tacgggaagg tatcagagtg gccagatgca ctgtggcacg tctcatggcg gttatgggac
       61 ttgccggtgt tctccggggt aaaaaggtcc gtacgaccat cagccggaaa gccgttgccg
      121 caggcgaccg cgtaaaccgt cagttcgtgg cagaacgacc tgaccagctg tgggtggctg
      181 attttactta cgtcagcaca tggcagggct tcgtctatgt ggcgttcatt attgatgtgt
      241 ttgccggata catcgtgggg tggcgggtct catcgtccat ggaaacgaca ttcgtgctgg
      301 atgc
//
LOCUS       DAJTOR010000452         5558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.guided.219, whole genome shotgun sequence.
ACCESSION   DAJTOR010000452 DAJTOR010000000
VERSION     DAJTOR010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.guided.219"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            980..1795
                     /gene="sul2"
                     /locus_tag="OO249_004392"
     CDS             980..1795
                     /gene="sul2"
                     /locus_tag="OO249_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5638416.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1882..2184
                     /locus_tag="OO249_004393"
     CDS             1882..2184
                     /locus_tag="OO249_004393"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638417.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2360..3853)
                     /locus_tag="OO249_004394"
     CDS             complement(2360..3853)
                     /locus_tag="OO249_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCR5638418.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(3897..4286)
                     /locus_tag="OO249_004395"
     CDS             complement(3897..4286)
                     /locus_tag="OO249_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5638419.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4504..4944
                     /locus_tag="OO249_004396"
     CDS             4504..4944
                     /locus_tag="OO249_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5638420.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1291 a         1557 c         1461 g         1249 t
ORIGIN      
        1 tgactcgcta cgctcggtcg ttcggctgcg gcgagcggta ccggcttacg gacggggacg
       61 gaaatttcct ggaagatacc aggaagacac ttagcaaaga agagataagg ccacggcgaa
      121 gccgtttttc cataggctcc gcccccctga caagcatcgc aaaatctgac gctcaaatca
      181 gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct
      241 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
      301 gtttctcatt ccacgcctga cactcagttc cgtgaaggca gttcgctcca agctggactg
      361 tatgcacgaa ccccccgttc agtccgaccg ctgcgccttg tccggtaact atcaacttga
      421 gtccaacccg gaaagacacg acaaaacgcc actggcagca gccattagta acaggagtgc
      481 acgagaagat agattaagag gtcttgaagt agaggcctga ctgcggctac actgaaagaa
      541 cagttttggt aatggcggtc tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga
      601 cttaaccttc gaacaaaccg cctccccagg cggttttttc gtttacagag caagagatta
      661 cgattcattg aaaaggatct caagaagatc ctttgtagat tactatcaat taatcttccg
      721 aacatgacac cagtcactgt tgattacggt taaaccgctc cgtaacaggt ttccctcgta
      781 ttggtgactt gcagcaccaa agaagcaaaa aagataaata tatctactta ttgagagatg
      841 atatatttat ttccaaaatg ctacagatac atcatcatga aacggatcac cgcaaacagg
      901 ttactcgcct ggggattccc tttcgacccg agcatccgta tgagactcat gctcgattat
      961 tattattata gaagccccca tgaataaatc gctcatcatt ttcggcatcg tcaacataac
     1021 ctcggacagt ttctccgatg gaggccggta tctggcgcca gacgcagcca ttgcgcaggc
     1081 gcgtaagctg atggccgagg gggcagatgt gatcgacctc ggtccggcat ccagcaatcc
     1141 cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc
     1201 gctcaaggca gatggcattc ccgtctcgct cgacagttat caacccgcga cgcaagccta
     1261 tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc ggttttccag acgctgcgtt
     1321 ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt atgcattcgg tgcaagacgg
     1381 gcaggcagat cggcgcgagg cacccgctgg cgacatcatg gatcacattg cggcgttctt
     1441 tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga
     1501 tcccggcatg ggtttttttc tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg
     1561 gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc
     1621 ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc ggggccgcga cactcgctgc
     1681 agagcttgcc gccgccgcag gtggagctga cttcatccgc acacacgagc cgcgcccctt
     1741 gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc gcaagaattc gttaactgca
     1801 cattcgggat atttctctat attcgcggtt cagcaggcat gtcccctttg agggcgaccc
     1861 gacgacagga taatcgacct tatggtgcgc aaatatttcg gcacagacgg tattcgtggc
     1921 aaagccaacg aaggcgcgat gacggcggaa accgccttgc gcgtcggcat ggcggctggc
     1981 cgtgtctttc gtcgcggtga ccaccgccat cgtgtcgtga tcggcaagga tacgcgcctg
     2041 tcgggctata tgcttgaacc cgcgctcaca gccggtttca cctcgatggg catggacgta
     2101 ttcctttttg gcccgctgcc gacaacgtat aggaagaata aacgcccttt tcacccaagt
     2161 ccaacagctt tggaccgcag ttgactcttt cgacacccct gcgatgcaac ccaatccggc
     2221 tgacggggag ccagcaacgc tgaaaattta ccctcctctt tcccactagc ggctcctttt
     2281 ccgacaacca gcacggcgga tccctgccgc ggcgctgtga acgcagcatt ttgattggta
     2341 tcgttggcct tcaggctcgt cagtcaaaca gacccaggag cagctcagcc ggtggcgccc
     2401 ggctttcggg taacgccctg gtcccgctgg tttcggcttt gtgcttcagg tgatcaagga
     2461 tctgcttgat cactataggg tcttcaatgc aggcgatgac tttcatggcg ccgccgcagc
     2521 cgctgcaggt ctcgatgtcg atattgaaaa cacgcttgag ccgttgcgcc catgtcatcg
     2581 acgctcgccg ttgtgctggt gttgccggtt catcagccac cctgaccttg ttgcccctgc
     2641 cccgttttgc cggcgtgacc aacgcccggt gccgactgtt gggtgcgaac accccgtgga
     2701 agcgggttag gttgactctg ggcttcggta ccagggcggc cagccttgca atgaaatcca
     2761 atggttcgaa aatgacgtgc gtggtgccgt cccggtacgg cgtcttgagc tggtagcgca
     2821 cgttgccgcc tcgtgttaac gacagccgct tctcggatac cgccgggcgg ctgatgtacc
     2881 ggcacagccg ttcgagcttc ttgcgttcat cggccctggc cgccacgccg gcgtgcaggc
     2941 tggacccggc taccttgcca atcccgtcac cgaacggatc accactggtc ggcagagttt
     3001 gcaaagtgaa cacctttcgc cccgcctgtg aaccgacagc gatacggtaa gtgatcgagt
     3061 gccccagcag gggtgtcatc gggtcgtcat ccaccgcatc cgaggccaga tagctgtttt
     3121 cgacatcccg ttccagcagg ccttgccgtt ccagatagcg acccacccgg tgggcgatgg
     3181 tgtgcgtcag ctgggtgagc tctgggctgg tcggcgcctt gacccagcgg aaacgcgctg
     3241 agccgtggga ttgctcgaca tacacaccgt cgagaaacag catgtggaag tgaacattca
     3301 gattgagcgc cgatccaaaa cgctggatca gggtgaccgc gcccgtcttg gccacttggt
     3361 gggtatggcc cgctttcttg accaggtgcg tggcaatgac gcggtaaacg atgcccagca
     3421 cccaccccat gatctcgggc cggctggcaa acaggaaacg cagctgaaac gggaagctca
     3481 acacccactg acgcatgggt tgttcaggca gtacttcatc aaccagcaag gcggcacttt
     3541 cggccatccg ccgcgcccca cagctcgggc agaaaccgcg acgcttacag ctgaaagcga
     3601 ccaggtgctc ggcgtggcaa gactcgcagc gaacccgtag aaagccatgc tccagccgcc
     3661 cgcattggag aaattcttca aattcccgtt gcacatagcc cggcaattcc tttccctgct
     3721 ctgccataag cgcagcgaat gccgggtaat actcgtcaac gatctgatag agaagggttt
     3781 gctcgggtcg gtggctctgg taacgaccag tatcccgatc ccggctggcc gtcctggccg
     3841 ccacatgagg catgttccgc gtccttgcaa tactgtgttt acatacagtc tatcgcttag
     3901 cggaaagttc ttttaccctc agccgaaatg cctgccgttg ctagacattg ccagccagtg
     3961 cccgtcactc ccactcttac attgttcgca gtatctaggt acatgttatt gcgtgcacct
     4021 tcatgatgta taatgttatt aatgacagca gaaagatcga cagaattaat attcttcgta
     4081 cctgggacag ttatcgggtg tcggccagtg atgagaccaa aggatgcagt ataagatgaa
     4141 ccactactat tatcatggcc accattacca ccaccgttaa aactacctcc cgaacttcca
     4201 gagccgctat gaccatcatt gtaggggtca tttggtccca tgtgaccaaa accaccgaca
     4261 gtaccgctat ttaactctgt actcataaaa ttatcctctt tgacttttaa aaacagcaaa
     4321 aactgtattt ataacaatgt gtttatatac agtattcgaa atactacgcc cgtcaaaacc
     4381 acaccgtcaa caggtttttt gtgtattttt cacgcctcac gggcagcctc agaccttttg
     4441 tgttaaaaca gtaatatgtt ctgctgttac aatacggtac acacaggaga cactgtcact
     4501 tttatggcat ggcacacagt tcaagagaca ctgaaattaa caggaaagtc gcgctcgcaa
     4561 ttatatcgtg acatggctaa aggccttgtc agctatcgca caggaaaaaa tgatcgccgg
     4621 gagttcgaga ccagtgaact gattcgggcc tatggcgagc tgcgcacttc tgagacacca
     4681 aaaaggcaca aggagggaca ctccaaatct gtcgttagcc aggcggaaaa tgctcagcta
     4741 gacgtcattc agcagaaact ggacgctctg cagcagactg ttaccctaat gcttgaagac
     4801 aagatggcgc gggaagcaga aaaccgcctg cgaaatgagg agagggaaca actacaggcc
     4861 gaaattagcc gtctggctga cgcgctggca caggaaaaaa agcggggatt ctggtcgcgg
     4921 ctgtttcgct ccggttcaga gtaacagtgc aggtgactct gatttaccat aacatctgtt
     4981 atcagcaccg cctgagaggc cctcagggcg tttctgacgg gaaaccggta aaacaggcaa
     5041 caaaccaccc gaaaacccgc cagaaacgcg caggctctgt ttttacggca tgcatgagta
     5101 tgcgcacaag tgcatagcgc ggaaattcac accattcagc cggggtaagt cctgtttccg
     5161 gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg
     5221 ttgccggcgc agccggaaaa cccgctgtca gcacgttcag gaagggggtg atgtacaaat
     5281 ggcacagagg atgaaagtga cgtatgagct gaaggaatga tgtaaaagtg atgtataagt
     5341 gatgtatgaa ctgaaggagc gatgtaattc tgtagtcagg aagaataacc cgttcatgca
     5401 ggcatgaacg atacagtgag gaacgctttt tttgtcacga tctcccctta acctgcgtaa
     5461 atcacccgcc atggttgatt atttttcaac ctcaaaatgg tcatacacct aacgcagccg
     5521 cgattttcat cgcaactgca tgaattaaaa gaaaaaaa
//
LOCUS       DAJTOR010000453         5440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.guided.221, whole genome shotgun sequence.
ACCESSION   DAJTOR010000453 DAJTOR010000000
VERSION     DAJTOR010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.guided.221"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            159..1967
                     /gene="frdA"
                     /locus_tag="OO249_004397"
     CDS             159..1967
                     /gene="frdA"
                     /locus_tag="OO249_004397"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5638421.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            1960..2694
                     /gene="frdB"
                     /locus_tag="OO249_004398"
     CDS             1960..2694
                     /gene="frdB"
                     /locus_tag="OO249_004398"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5638422.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2705..3100
                     /gene="frdC"
                     /locus_tag="OO249_004399"
     CDS             2705..3100
                     /gene="frdC"
                     /locus_tag="OO249_004399"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5638423.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3111..3470
                     /gene="frdD"
                     /locus_tag="OO249_004400"
     CDS             3111..3470
                     /gene="frdD"
                     /locus_tag="OO249_004400"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5638424.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            3533..4666
                     /locus_tag="OO249_004401"
     CDS             3533..4666
                     /locus_tag="OO249_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5638425.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4755..5288
                     /gene="blc"
                     /locus_tag="OO249_004402"
     CDS             4755..5288
                     /gene="blc"
                     /locus_tag="OO249_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5638426.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5285..>5440)
                     /gene="sugE"
                     /locus_tag="OO249_004403"
     CDS             complement(5285..>5440)
                     /gene="sugE"
                     /locus_tag="OO249_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5638427.1"
                     /translation="VGTAYAVWTGIGAVGAAITGIVLLGESAHPMRLASLALIVLGII
                     GLKLSTH"
BASE COUNT         1321 a         1426 c         1468 g         1225 t
ORIGIN      
        1 gcactaaaaa aatcgatctc gtcaaatttc agacttatcc atcagactat actgttgtac
       61 ctataaagga gcagtggaat agcgttcgca gaccgtaact ttcaggcact taccctgaag
      121 tacggggctg tgggataaaa acaatctgga ggaatgtcgt gcaaaccttt caagccgatc
      181 ttgccattgt aggcgccggt ggcgcgggat tacgtgctgc aattgctgcc gcgcaggcaa
      241 atccaaatgc aaaaatcgca ctaatctcaa aagtataccc gatgcgtagc cataccgttg
      301 ctgcagaagg gggctccgcc gctgtcgcgc aggatcatga cagcttcgaa tatcactttc
      361 acgatacagt agcgggtggc gactggttgt gtgagcagga tgtcgtggat tatttcgtcc
      421 accactgccc aaccgaaatg acccaactgg aactgtgggg gtgcccatgg agccgtcgcc
      481 cggatggtag cgtcaacgta cgtcgcttcg gcggcatgaa aatcgagcgc acctggttcg
      541 ccgccgataa gaccggcttc catatgctgc acacgctgtt ccagacctct ctgcaattcc
      601 cgcagatcca gcgttttgac gaacatttcg tgctggatat tctggttgat gatggtcatg
      661 ttcgcggcct ggtagcaatg aacatgatgg aaggcacgct ggtgcagatc cgtgctaacg
      721 cggtcgttat ggctactggc ggtgcgggtc gcgtttatcg ttacaacacc aacggcggca
      781 tcgttaccgg tgacggtatg ggtatggcgc taagccacgg cgttccgctg cgtgacatgg
      841 aattcgttca gtatcaccca accggtctgc caggttccgg tatcctgatg accgaaggct
      901 gccgtggtga aggcggtatt ctggtcaaca aaaatggcta ccgttatctg caagattacg
      961 gcatgggccc ggaaactccg ctgggcgagc cgaaaaacaa atatatggaa ctgggtccac
     1021 gcgacaaagt ttctcaggcc ttctggcacg aatggcgtaa aggcaacacc atctccacgc
     1081 cgcgtggcga tgtggtttat ctcgacttgc gtcacctcgg cgagaaaaaa ctgcatgaac
     1141 gtctgccgtt catctgcgaa ctggcgaaag cgtacgttgg cgtcgatccg gttaaagaac
     1201 cgattccggt acgtccgacc gcacactaca ccatgggcgg tatcgaaacc gatcagaact
     1261 gtgaaacccg cattaaaggt ctgttcgccg tgggtgaatg ttcctctgtt ggtctgcacg
     1321 gtgcaaaccg tctgggctcc aactccctgg cggaactggt ggtcttcggc cgtatggccg
     1381 gtgaacaagc gacagagcgt gcagcaactg ccggtaatgg caacgaagag gcaattgaag
     1441 cgcaggcagc tggcgttgaa caacgtctga aagatctggt taaccaggat ggcggcgaaa
     1501 actgggcgaa gatccgcgac gaaatgggcc tagcaatgga agaaggttgc ggtatctacc
     1561 gtacgccgga actgatgcag aaaaccatcg acaagctggc agagctgcag gaacgcttca
     1621 agcgcgtgcg catcaccgac acctccagcg tgttcaacac cgacctgctc tacaccattg
     1681 aactgggcca cggtctgaac gttgctgaat gtatggcgca ctccgcaatg gcacgtaaag
     1741 agtcccgcgg cgcacaccag cgtctggacg aaggttgcac cgagcgtgac gacgtcaact
     1801 tcctcaaaca caccctcgcc ttccgcgatg ctgatggcac gactcgcctg gagtacagcg
     1861 acgtgaagat tactacgctg ccgccagcta aacgcgttta cggtggcgaa gcggatgcag
     1921 ccgataaggc ggaagcagcc aataagaagg agaaggcgaa tggctgagat gaaaaacctg
     1981 aaaattgagg tggtgcgcta taacccggaa gtcgataccg caccgcatag cgcattctat
     2041 gaagtgcctt atgacgcaac tacctcatta ctggatgcgc tgggctacat caaagacaac
     2101 ctggcaccgg acctgagtta ccgctggtcc tgccgtatgg cgatttgtgg ttcctgcggc
     2161 atgatggtta acaacgtgcc aaaactggca tgtaaaacct tcctgcgtga ttacaccgac
     2221 ggtatgaagg ttgaagcgtt agctaacttc ccgattgaac gcgatctggt ggtcgatatg
     2281 acccacttca tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc
     2341 gcggatcagg gtactaacat ccagaccccg gcgcagatgg caaagtatca ccagttctcc
     2401 ggttgcatca actgtggtct gtgctacgcc gcgtgcccgc agtttggcct gaacccagag
     2461 ttcatcggtc cggctgccat tacgctggcg catcgttata acgaagatag ccgcgaccac
     2521 ggtaagaagg agcgtatggc gcagttgaac agccagaacg gcgtatggag ctgtactttc
     2581 gtgggctatt gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc cattcagcag
     2641 ggcaaagtag aaagttcgaa agactttctt atcgcgaccc tgaaaccacg ctaaggagtg
     2701 caacatgacg actaaacgta aaccgtatgt acggccaatg acgtccacct ggtggaaaaa
     2761 attgccgttt tatcgctttt acatgctgcg cgaaggcacg gcggttccgg ctgtgtggtt
     2821 cagcattgaa ctgattttcg ggctgtttgc cctgaaaaat ggcccggacg cctgggcggg
     2881 attcgtcgac tttttacaaa acccggttat cgtgatcatt aacctgatca ctctggcggc
     2941 agctctgctg cacaccaaaa cctggtttga actggcaccg aaagcggcca atatcattgt
     3001 aaaagacgaa aaaatgggac cagagccaat tatcaaaagt ctctgggcgg taactgtggt
     3061 tgccaccatc gtaatcctgt ttgttgccct gtactggtaa ggagcctgag atgattaatc
     3121 caaatccaaa gcgttctgac gaaccggtat tctggggcct cttcggggcc ggtggtatgt
     3181 ggagcgcaat tattgcgccg gtgatgatcc tgctggtggg tattctgctg ccactggggc
     3241 tgtttccggg tgatgcgctg agctacgagc gcgttctggc gttcgcgcag agcttcattg
     3301 gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg gtgtggttta caccgtatgc
     3361 accacgcgat gcacgatctg aaaattcacg tgcctgcggg caaatgggtt ttctacggtc
     3421 tggctgctat cctgacagtt gtcacgctga ttggtatcgt tacaatctaa cgtatcgcca
     3481 atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg aaaccagacc ttatgttcaa
     3541 aacgacgctc tgcgccttat taattaccgc ctcttgctcc acatttgctg cccctcaaca
     3601 aatcaacgat attgtgcatc gcacaattac cccgcttata gagcaacaaa agatccccgg
     3661 tatggcggtg gcggtaattt atcagggtaa accttattac tttacctggg gctatgcgga
     3721 catcgccaaa aagcagcccg tcacacagca aacgttgttt gagttaggtt cggtcagcaa
     3781 aacatttacg ggcgtgcttg gtggcgatgc tattgctcga ggggaaatca agttaagcga
     3841 tcccgcaaca aaatactggc ctgaacttac cgctaaacag tggaatggga tcacactatt
     3901 acatcttgcg acctacaccg ctggcggcct gccattgcag atgccggatg aagtgaaatc
     3961 ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc caggaacaca
     4021 acgtctgtat gccaactcca gtatcggttt gttcggcgca ctggctgtga agccgtctgg
     4081 tttgagtttt gagcaggcga tgcaaactcg tgtcttccag ccactcaaac tcaaccatac
     4141 gtggattaat gtaccgcccg cagaagaaaa gaattacgcc tggggatatc gcgaaggcaa
     4201 ggcagttcat gtttcgccag gggcgttaga tgctgaagct tatggtgtga agtcgaccat
     4261 tgaagatatg gcccgctggg tgcaaagcaa tttaaccccc cttgatatca atgagaaaac
     4321 gcttcaacaa gggatacaac tggcacaatc tcgctactgg caaaccggcg atatgtatca
     4381 gggcctgggc tgggaaatgc tggactggcc ggtcaatcct gacagcatca ttaacggcag
     4441 tgacaataaa attgcactgg cagcacgccc ggtaaaagcg attaagcccc caactcctgc
     4501 agtacgcgca tcatgggtac ataaaacggg ggcgaccggc ggatttggta gctatgtcgc
     4561 gtttattcca gaaaaagagc tgggtatcgt gatgctggct aacaaaaact atcccaatcc
     4621 agcgagagtc gccgccgcct gtcagattct caacgctcta cagtaaaatt tcatcgggtc
     4681 cgaattttcg gaccttttct gcgcttttcc ttgctgtcat ctacacttag aaaaaaacca
     4741 gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc agcgacagct gcatttctgg
     4801 tcgttgcctg cagttctcct acgccgccgc gtggcgtgac agtggtgaat aatttcgacg
     4861 ctaaacgcta tctcggtacc tggtatgaga ttgcccgttt tgatcaccgt tttgaacgtg
     4921 gactggaaaa agtcaccgca acatacagtc tgcgtgatgg cggcggcctg aatgtcatca
     4981 ataaaggcta taaccctgac agagaaatgt ggcagcagag tgaagggaaa gcgtacttta
     5041 ccggcgcacc aacacgcgct gcgctgaaag tgtcattctt tggtcctttc tatggcggtt
     5101 ataacgtcat tgcacttgat cgggaatacc gccatgcgct ggtttgcggc ccggaccgcg
     5161 actacctgtg gatactctcc cgcacgccaa ccatttctga cgaagtgaaa caggagatgc
     5221 tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt tatttgggta cagcagcccg
     5281 gtagttagtg agtgctgagt ttcagaccaa taatccccaa tacgattaac gccagactcg
     5341 ccaggcgcat cgggtgagcg gactcaccga gcagcacaat gccggtgata gccgcgccga
     5401 ctgcgccaat acccgtccac acggcataag ccgtccctac
//
LOCUS       DAJTOR010000454         4298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.guided.242, whole genome shotgun sequence.
ACCESSION   DAJTOR010000454 DAJTOR010000000
VERSION     DAJTOR010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.guided.242"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            561..800
                     /locus_tag="OO249_004404"
     CDS             561..800
                     /locus_tag="OO249_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5638428.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(988..1965)
                     /gene="intI2"
                     /locus_tag="OO249_004405"
                     /pseudo
     CDS             complement(988..1965)
                     /gene="intI2"
                     /locus_tag="OO249_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2297..2770
                     /locus_tag="OO249_004406"
     CDS             2297..2770
                     /locus_tag="OO249_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5638429.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2865..3389
                     /gene="sat2"
                     /locus_tag="OO249_004407"
     CDS             2865..3389
                     /gene="sat2"
                     /locus_tag="OO249_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5638430.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3447..4238
                     /gene="aadA1"
                     /locus_tag="OO249_004408"
     CDS             3447..4238
                     /gene="aadA1"
                     /locus_tag="OO249_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5638431.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1208 a          943 c         1004 g         1143 t
ORIGIN      
        1 aaagagccag tcagttgctt aactgactgg cattacaccg gtcggcagcc ctgtgggcgg
       61 acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta tttagggaag
      121 agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg
      181 acaaaatagt ttggaactag atttcactta tctggttaac taatctagct aacctgccgc
      241 tcagcttagt acgatcctat atccaactga tcataatcct ttcatcagta tcccaaatta
      301 aatccccctc ctagactgcc tcaccaacac tttacagtga tcccgtattt ccggacaggt
      361 gattgatttt tttcgcttgt acaggtgtta agccacagtt atcttgatgt ggacattatg
      421 cattgtaata gcccattaac atgcgctaag agcataaatt aatatacagt aacaagcatt
      481 acgaggtgct aatgtttgga aagcagcctc ttccaactac aataataagt taactatcct
      541 ccacccacaa ataattacaa atgacccctt tccgctataa ctccgatctc accagcggct
      601 cgctacaaac ccgagagtgt cgcattatta ctggcttgtt actccaagag cttgatgaag
      661 ctgcctggga taaagccatg tataaggaaa atgtgcttca gaaacgcacg caatctacgg
      721 taagacgtat ttcttcagcg cttagaaaac gcctagaaca cctaagctct gacttttggg
      781 cttttgcgtt tttatgttag gcattgtctg gcaactggag gaagtgtaat gaccatgaat
      841 ggtcactgtg attgacagta tcttgcttaa acttatctga acaatggtag aactggctca
      901 tatttatccc ctctatgatt gtttatcttt ttcaccataa aaagtatcag gggctactca
      961 gggctacata aagatattta tctactttta ctgattgata agtagcatca gtccatccgc
     1021 aggactggtg gtgccggcaa aatgctgacc caacacatgc gtatagattt gcgtggtctt
     1081 aacatcgtta tgccctaaga gttcttgcac agtgcgaata tcacgccccg cttgtaatag
     1141 atgcgtagca aacgagtgac gaaatgtatg acaagtgaca cgcttgctaa cgatgcctgc
     1201 tttttgtacg gctgccttca atgcctttcg cgcaacggag tcatgcagat gatggcggca
     1261 taatttgccg ttatacgggt ggttgcagag cgtgctggag ggaaagacaa acatccacgc
     1321 cgcttgtcga taagcagaag ggtatttgtg atctaaagca aaaggcagcg atggccctac
     1381 gccttgtaag ttgtcgtctt gctgaataag ccgcgcttgc tcaatgagtt attttattgc
     1441 tgggattagg cgcgtgggca gtaggctgtt tctgcttttc ccacccttac cgtcatgcac
     1501 agtgatgcag ccattatcaa aatcaaaatc tttaacccgc aaacgcaagc attcattaat
     1561 gcgcaaacct gcaccataca gcagcgtaaa aataacttgg ttgcgagtat ccataacctg
     1621 caaaatgcgt tgcacttcat ttgcagagat aacagagggt agccgtctag gcttgcttgc
     1681 agggatataa tcaatatcgc ccaacggctg ttgtaaaaac ctgttgtaca aaaaagctag
     1741 ggcatttaaa gcgattttct gcgtgtttat ggctacatgt ctgctgtttg ctaagctgga
     1801 taaaaacagc ctgacctctt cactgcccat ggtctgagga tgacgttttt tgtgaaacag
     1861 aataaaacgc ttaatccagt gcaggtaagt tttttcagtt ttcagcgcat aacctttttg
     1921 tcgcatatcc gtgcgtatag aatttaaaaa tggactgtta gacataaaac gctccttgtc
     1981 ttgcaactgt ctgcctatac agcctattct agctgggatt taaaaaagtg cctgtttttt
     2041 acgcctagag atgcttgttt accggtagag ttttaattta atgctaaata aattaaaatg
     2101 ttatgagttc tttgggtgag ataatgtgca tcgtgcaagc aggatagacg gcatgcacga
     2161 tttgtaataa cagagtgtct tgtattttta aagaaagtct atttaataca agtgattata
     2221 ttaattaacg gtaagcatca gcgggtgaca aaacgagcat gcttactaat aaaatgttaa
     2281 cctctgagga agaattgtga aactatcact aatggtagct atatcgaaga atggagttat
     2341 cgggaatggc cctgatattc catggagtgc caaaggtgaa cagctcctgt ttaaagctat
     2401 tacctataac caatggctgt tggttggacg caagactttt gaatcaatgg gagcattacc
     2461 caaccgaaag tatgcggtcg taacacgttc aagttttaca tctgacaatg agaacgtatt
     2521 gatctttcca tcaattaaag atgctttaac caacctaaag aaaataacgg atcatgtcat
     2581 tgtttcaggt ggtggggaga tatacaaaag cctgatcgat caagtagata cactacatat
     2641 atctacaata gacatcgagc cggaaggtga tgtttacttt cctgaaatcc ccagcaattt
     2701 taggccagtt tttacccaag acttcgcctc taacataaat tatagttacc aaatctggca
     2761 aaagggttaa caagtggcag caacggattc gcaaacctgg cgcggctttt gtaccaaaag
     2821 ccgcgccagg tttgcgatcc gctgtgccag gcgttaggcg tcatatgaag atttcggtga
     2881 tccctgagca ggtggcggaa acattggatg ctgagaacca tttcattgtt cgtgaagtgt
     2941 tcgatgtgca cctatccgac caaggctttg aactatctac cagaagtgtg agcccctacc
     3001 ggaaggatta catctcggat gatgactctg atgaagactc tgcttgctat ggcgcattca
     3061 tcgaccaaga gcttgtcggg aagattgaac tcaactcaac atggaacgat ctagcctcta
     3121 tcgaacacat tgttgtgtcg cacacgcacc gaggcaaagg agtcgcgcac agtctcatcg
     3181 aatttgcgaa aaagtgggca ctaagcagac agctccttgg catacgatta gagacacaaa
     3241 cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg ctttactctc ggcggcattg
     3301 acctgttcac gtataaaact agacctcaag tctcgaacga aacagcgatg tactggtact
     3361 ggttctcggg agcacaggat gacgcctaac aattcattca agccgacacc gcttcgcggc
     3421 gcggcttaat tcaggagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
     3481 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     3541 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     3601 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     3661 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     3721 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     3781 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     3841 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     3901 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     3961 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     4021 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     4081 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     4141 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact
     4201 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc
     4261 gacgccgctt cgcggcgcgg cttaactcaa gcgttaga
//
LOCUS       DAJTOR010000455         4295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.guided.243, whole genome shotgun sequence.
ACCESSION   DAJTOR010000455 DAJTOR010000000
VERSION     DAJTOR010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.guided.243"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            561..800
                     /locus_tag="OO249_004409"
     CDS             561..800
                     /locus_tag="OO249_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5638432.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(988..1965)
                     /gene="intI2"
                     /locus_tag="OO249_004410"
                     /pseudo
     CDS             complement(988..1965)
                     /gene="intI2"
                     /locus_tag="OO249_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2297..2770
                     /locus_tag="OO249_004411"
     CDS             2297..2770
                     /locus_tag="OO249_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5638433.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2865..3389
                     /gene="sat2"
                     /locus_tag="OO249_004412"
     CDS             2865..3389
                     /gene="sat2"
                     /locus_tag="OO249_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5638434.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3447..4235
                     /gene="aadA1"
                     /locus_tag="OO249_004413"
     CDS             3447..4235
                     /gene="aadA1"
                     /locus_tag="OO249_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5638435.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1205 a          943 c         1004 g         1143 t
ORIGIN      
        1 aaagagccag tcagttgctt aactgactgg cattacaccg gtcggcagcc ctgtgggcgg
       61 acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta tttagggaag
      121 agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg
      181 acaaaatagt ttggaactag atttcactta tctggttaac taatctagct aacctgccgc
      241 tcagcttagt acgatcctat atccaactga tcataatcct ttcatcagta tcccaaatta
      301 aatccccctc ctagactgcc tcaccaacac tttacagtga tcccgtattt ccggacaggt
      361 gattgatttt tttcgcttgt acaggtgtta agccacagtt atcttgatgt ggacattatg
      421 cattgtaata gcccattaac atgcgctaag agcataaatt aatatacagt aacaagcatt
      481 acgaggtgct aatgtttgga aagcagcctc ttccaactac aataataagt taactatcct
      541 ccacccacaa ataattacaa atgacccctt tccgctataa ctccgatctc accagcggct
      601 cgctacaaac ccgagagtgt cgcattatta ctggcttgtt actccaagag cttgatgaag
      661 ctgcctggga taaagccatg tataaggaaa atgtgcttca gaaacgcacg caatctacgg
      721 taagacgtat ttcttcagcg cttagaaaac gcctagaaca cctaagctct gacttttggg
      781 cttttgcgtt tttatgttag gcattgtctg gcaactggag gaagtgtaat gaccatgaat
      841 ggtcactgtg attgacagta tcttgcttaa acttatctga acaatggtag aactggctca
      901 tatttatccc ctctatgatt gtttatcttt ttcaccataa aaagtatcag gggctactca
      961 gggctacata aagatattta tctactttta ctgattgata agtagcatca gtccatccgc
     1021 aggactggtg gtgccggcaa aatgctgacc caacacatgc gtatagattt gcgtggtctt
     1081 aacatcgtta tgccctaaga gttcttgcac agtgcgaata tcacgccccg cttgtaatag
     1141 atgcgtagca aacgagtgac gaaatgtatg acaagtgaca cgcttgctaa cgatgcctgc
     1201 tttttgtacg gctgccttca atgcctttcg cgcaacggag tcatgcagat gatggcggca
     1261 taatttgccg ttatacgggt ggttgcagag cgtgctggag ggaaagacaa acatccacgc
     1321 cgcttgtcga taagcagaag ggtatttgtg atctaaagca aaaggcagcg atggccctac
     1381 gccttgtaag ttgtcgtctt gctgaataag ccgcgcttgc tcaatgagtt attttattgc
     1441 tgggattagg cgcgtgggca gtaggctgtt tctgcttttc ccacccttac cgtcatgcac
     1501 agtgatgcag ccattatcaa aatcaaaatc tttaacccgc aaacgcaagc attcattaat
     1561 gcgcaaacct gcaccataca gcagcgtaaa aataacttgg ttgcgagtat ccataacctg
     1621 caaaatgcgt tgcacttcat ttgcagagat aacagagggt agccgtctag gcttgcttgc
     1681 agggatataa tcaatatcgc ccaacggctg ttgtaaaaac ctgttgtaca aaaaagctag
     1741 ggcatttaaa gcgattttct gcgtgtttat ggctacatgt ctgctgtttg ctaagctgga
     1801 taaaaacagc ctgacctctt cactgcccat ggtctgagga tgacgttttt tgtgaaacag
     1861 aataaaacgc ttaatccagt gcaggtaagt tttttcagtt ttcagcgcat aacctttttg
     1921 tcgcatatcc gtgcgtatag aatttaaaaa tggactgtta gacataaaac gctccttgtc
     1981 ttgcaactgt ctgcctatac agcctattct agctgggatt taaaaaagtg cctgtttttt
     2041 acgcctagag atgcttgttt accggtagag ttttaattta atgctaaata aattaaaatg
     2101 ttatgagttc tttgggtgag ataatgtgca tcgtgcaagc aggatagacg gcatgcacga
     2161 tttgtaataa cagagtgtct tgtattttta aagaaagtct atttaataca agtgattata
     2221 ttaattaacg gtaagcatca gcgggtgaca aaacgagcat gcttactaat aaaatgttaa
     2281 cctctgagga agaattgtga aactatcact aatggtagct atatcgaaga atggagttat
     2341 cgggaatggc cctgatattc catggagtgc caaaggtgaa cagctcctgt ttaaagctat
     2401 tacctataac caatggctgt tggttggacg caagactttt gaatcaatgg gagcattacc
     2461 caaccgaaag tatgcggtcg taacacgttc aagttttaca tctgacaatg agaacgtatt
     2521 gatctttcca tcaattaaag atgctttaac caacctaaag aaaataacgg atcatgtcat
     2581 tgtttcaggt ggtggggaga tatacaaaag cctgatcgat caagtagata cactacatat
     2641 atctacaata gacatcgagc cggaaggtga tgtttacttt cctgaaatcc ccagcaattt
     2701 taggccagtt tttacccaag acttcgcctc taacataaat tatagttacc aaatctggca
     2761 aaagggttaa caagtggcag caacggattc gcaaacctgg cgcggctttt gtaccaaaag
     2821 ccgcgccagg tttgcgatcc gctgtgccag gcgttaggcg tcatatgaag atttcggtga
     2881 tccctgagca ggtggcggaa acattggatg ctgagaacca tttcattgtt cgtgaagtgt
     2941 tcgatgtgca cctatccgac caaggctttg aactatctac cagaagtgtg agcccctacc
     3001 ggaaggatta catctcggat gatgactctg atgaagactc tgcttgctat ggcgcattca
     3061 tcgaccaaga gcttgtcggg aagattgaac tcaactcaac atggaacgat ctagcctcta
     3121 tcgaacacat tgttgtgtcg cacacgcacc gaggcaaagg agtcgcgcac agtctcatcg
     3181 aatttgcgaa aaagtgggca ctaagcagac agctccttgg catacgatta gagacacaaa
     3241 cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg ctttactctc ggcggcattg
     3301 acctgttcac gtataaaact agacctcaag tctcgaacga aacagcgatg tactggtact
     3361 ggttctcggg agcacaggat gacgcctaac aattcattca agccgacacc gcttcgcggc
     3421 gcggcttaat tcaggagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
     3481 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     3541 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     3601 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     3661 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     3721 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     3781 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     3841 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     3901 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     3961 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     4021 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     4081 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctagg caggcttatc
     4141 ttggacaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg
     4201 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac
     4261 gccgcttcgc ggcgcggctt aactcaagcg ttaga
//
LOCUS       DAJTOR010000456         3235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.guided.272, whole genome shotgun sequence.
ACCESSION   DAJTOR010000456 DAJTOR010000000
VERSION     DAJTOR010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.guided.272"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(<1..1123)
                     /gene="pepD"
                     /locus_tag="OO249_004414"
     CDS             complement(<1..1123)
                     /gene="pepD"
                     /locus_tag="OO249_004414"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HCR5638436.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDY"
     gene            1384..1842
                     /gene="gpt"
                     /locus_tag="OO249_004415"
     CDS             1384..1842
                     /gene="gpt"
                     /locus_tag="OO249_004415"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5638437.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1934..3178
                     /gene="frsA"
                     /locus_tag="OO249_004416"
     CDS             1934..3178
                     /gene="frsA"
                     /locus_tag="OO249_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5638438.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
BASE COUNT          769 a          817 c          832 g          817 t
ORIGIN      
        1 cgtagtcttt accgctgtcg atcagtgaac ggatcaggca gtgaatttct acgttattgt
       61 cagtcatggt caccacaccg acgttcaggg aggtttcaac cacacctttg gctacatcgg
      121 agttgcggat cacaccgttc ggggtggcgt tcagcagacg aataaaggta tcgcgagatt
      181 tcgcaatcag ggcagcttta tcgttcgcta cagagtccag caacaaggcc agatttttct
      241 ctttctctgc cagctcgttt ttcaggatct cctgataggt attcaccaga gatttcagtg
      301 cgtcgacttt atcagctgcg acagcaatgg tcgcaaaggc ttcacgcggg atggcgttac
      361 gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag ttcttctgca tgacccgcca
      421 ggaagcgcac cagcagtttg ttggcattac ccaggccaac gtggatttca ccgccggagt
      481 gaccgccttt cagacctttt aaggttaact tgaaggtttc aaaaccagct ggaaccgctt
      541 cacgatctaa atgcaggttg gaggtgaagt cgataccccc cgcacaaccc atgtagattt
      601 caccttcttc ttcggagtcg gtgttaatca gaatatcagc ctgcaaccag ttgctctgta
      661 agccaaaagc accgtccata ccggcttctt cggtcatggt cagcagcact tccagcggac
      721 cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga ggccatacca atgccgttat
      781 ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc atcaatataa ggctggatag
      841 gatctttcgt gaagtcatgc acggtgtcgt tatttttctg cggcaccata tcgaggtggg
      901 cctgtaagac gaccggttta cgattttcca tacctgcggt agcaggttta cgaatcagga
      961 tattacctac ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa ccaacaatgt
     1021 attcagcgag ttgctcttca tgataggacg ggtgcggaat agaacagatt ttggcaaaaa
     1081 tatcccacag cggctgtgga gataattgag acagttcaga cacgttaagt ctccttgtcg
     1141 atcacccgca aaacagtatt gcaggtcaca gggttagcag aaaatgttgt caacacaaga
     1201 caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg
     1261 taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc
     1321 gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt
     1381 cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc
     1441 gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt
     1501 ctggtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
     1561 atttccagct acgatcacga caaccagcga gagcttaaag tgctgaaacg cgcagaaggc
     1621 gatggcgaag gcttcatagt tattgatgac ctggtggata ccggtggtac tgctgttgcg
     1681 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
     1741 ccgctggttg atgactatgt tgttgatatt ccgcaagata cctggatcga acagccgtgg
     1801 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
     1861 ctgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag taagtattct
     1921 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
     1981 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
     2041 gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
     2101 gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta ttgtgatgag
     2161 cgatgctgaa cgtaccgacg atgatttata cgatactgtg attggctacc gtggtggcaa
     2221 ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct gtgcggaaga
     2281 agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca acattgccgc
     2341 ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat caaaccgcgc
     2401 ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt ttaccgtacc
     2461 cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc cgttcccgac
     2521 agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc tgtatgaacg
     2581 ttattttgcg ccgcgcggca ttgtgatgct gactattgat atgccgtcgg tgggcttttc
     2641 ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct taaaggcgct
     2701 gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc gtttcggcgc
     2761 taacgttgcc gtgcgtctgg catatcttga atcgccgcgt ctgaaagcgg ttgcctgtct
     2821 tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg tgccggaaat
     2881 gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg aagcgttacg
     2941 cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctgggacgtc gctgcccaac
     3001 gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg actcacgctt
     3061 aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc cggtgtatcg
     3121 gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct tgtgttaaaa
     3181 atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga tagca
//
LOCUS       DAJTOR010000457         2867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.guided.286, whole genome shotgun sequence.
ACCESSION   DAJTOR010000457 DAJTOR010000000
VERSION     DAJTOR010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.guided.286"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            complement(98..448)
                     /locus_tag="OO249_004417"
     CDS             complement(98..448)
                     /locus_tag="OO249_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5638439.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(651..1664)
                     /gene="intI1"
                     /locus_tag="OO249_004418"
     CDS             complement(651..1664)
                     /gene="intI1"
                     /locus_tag="OO249_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5638440.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1874..2704
                     /locus_tag="OO249_004419"
     CDS             1874..2704
                     /locus_tag="OO249_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5638441.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          774 a          755 c          721 g          617 t
ORIGIN      
        1 ctctatgacc ttcaacgaga tatgtcaata aattcaaaat tcaatcctat cctgacgcaa
       61 tttacacatg gcatctgaca tcaggttagg gtatgcctca acctgacggc ggcgaatcac
      121 aagcgtccgg tttgacgctg gtttcggtcg cagtgctggc ccgcgcctgg aagcgctgat
      181 agcactccag cccgcagaaa tgctccacgt actcggcccc ttccggcgtg aaggcggcat
      241 cgagcgggat ttccttgcag cacacgcagc aactggtgct cggttcattg gcgttcatgg
      301 tggtgttcct ccattggttg acgaagccga cgaaggccgc cgccggcatc ggcctggcga
      361 acaggaaacc ctgcaccgtg tcgcaacccg cctgccgcaa ccacgcaagg cagtcgggtg
      421 tttccacacc ctcggctacc acctccattg tcgttttcag aagacggctg cactgaacgt
      481 cagaagccga ctgcactata gcagcggagg ggttggatcc atcaggcaac gacgggctgc
      541 tgccggccat cagcggacgc agggaggact ttccgcaacc ggccgttcga tgcggcaccg
      601 atggccttcg cgcaggggta gtgaatccgc caggattgac ttgcgctgcc ctacctctca
      661 ctagtgaggg gcggcagcgc atcaagcggt gagcgcactc cggcaccgcc aactttcagc
      721 acatgcgtgt aaatcatcgt cgtagagacg tcggaatggc cgagcagatc ctgcacggtt
      781 cgaatgtcgt aaccgctgcg gagcaaggcc gtcgcgaacg agtggcggag ggtgtgcggt
      841 gtggcgggct tcgtgatgcc tgcttgttct acggcacgtt tgaaggcgcg ctgaaaggtc
      901 tggtcataca tgtgatggcg acgcacgaca ccgctccgtg gatcggtcga atgcgtgtgc
      961 tgcgcaaaaa cccagaacca cggccaggaa tgcccggcgc gcggatactt ccgctcaagg
     1021 gcgtcgggaa gcgcaacgcc gctgcggccc tcggcctggt ccttcagcca ccatgcccgt
     1081 gcacgcgaca gctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga
     1141 tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg
     1201 acccgcagtt gcaaaccctc actgatccgc atgcccgttc catacagaag ctgggcgaac
     1261 aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc
     1321 accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg cagatccgtg
     1381 cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg cgtggagacc
     1441 gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct gctgcccaag
     1501 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     1561 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     1621 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     1681 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     1741 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     1801 aagttgggcg aacccggagc ctcattaatt gttagccgtt aaaattaagc cctttaccaa
     1861 accaatactt attatgaaaa acacaataca tatcaacttc gctatttttt taataattgc
     1921 aaatattatc tacagcagcg ccagtgcatc aacagatatc tctactgttg catctccatt
     1981 atttgaagga actgaaggtt gttttttact ttacgatgca tccacaaacg ctgaaattgc
     2041 tcaattcaat aaagcaaagt gtgcaacgca aatggcacca gattcaactt tcaagatcgc
     2101 attatcactt atggcatttg atgcggaaat aatagatcag aaaaccatat tcaaatggga
     2161 taaaaccccc aaaggaatgg agatctggaa cagcaatcat acaccaaaga cgtggatgca
     2221 attttctgtt gtttgggttt cgcaagaaat aacccaaaaa attggattaa ataaaatcaa
     2281 gaattatctc aaagattttg attatggaaa tcaagacttc tctggagata aagaaagaaa
     2341 caacggatta acagaagcat ggctcgaaag tagcttaaaa atttcaccag aagaacaaat
     2401 tcaattcctg cgtaaaatta ttaatcacaa tctcccagtt aaaaactcag ccatagaaaa
     2461 caccatagag aacatgtatc tacaagatct ggataatagt acaaaactgt atgggaaaac
     2521 tggtgcagga ttcacagcaa atagaacctt acaaaacgga tggtttgaag ggtttattat
     2581 aagcaaatca ggacataaat atgtttttgt gtccgcactt acaggaaact tggggtcgaa
     2641 tttaacatca agcataaaag ccaagaaaaa tgcgatcacc attctaaaca cactaaattt
     2701 ataaaaaatc taatggcaaa atcgcccaac ccttcaatca agtcgggacg gccaaaagca
     2761 agcttttggc tcccctcgct ggcgctcggc gccccttatt tcaaacgtta aacatcatga
     2821 gggaagtggt gatcgccgaa gtatcgactc aactatcaga ggtagtt
//
LOCUS       DAJTOR010000458         2648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.guided.293, whole genome shotgun sequence.
ACCESSION   DAJTOR010000458 DAJTOR010000000
VERSION     DAJTOR010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.guided.293"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            21..644
                     /locus_tag="OO249_004420"
     CDS             21..644
                     /locus_tag="OO249_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5638442.1"
                     /translation="MSDISRVKILSALMDGRAWTATELSSVANISASTASSHLSKLLD
                     CQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHGVHAKVSTPVHLRKARTCYDHL
                     AGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSER
                     RFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(622..1245)
                     /gene="tetR(B)"
                     /locus_tag="OO249_004421"
     CDS             complement(622..1245)
                     /gene="tetR(B)"
                     /locus_tag="OO249_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5638443.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1327..2532
                     /gene="tet(B)"
                     /locus_tag="OO249_004422"
     CDS             1327..2532
                     /gene="tet(B)"
                     /locus_tag="OO249_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5638444.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          678 a          513 c          586 g          871 t
ORIGIN      
        1 tagccgcgat aggggcggct atgtctgaca tttcacgagt taaaatactc agtgctttga
       61 tggatgggcg agcttggacg gccactgagc taagttctgt ggcgaatata tcagcttcaa
      121 cggcgagcag tcatttatct aaattattag attgccagct aatcacagta gtagctcaag
      181 gcaagcatcg ttattttcgg ctagcaggaa aagatattgc tgaattgatg gaaagtatga
      241 tggggatctc cttaaaccat ggcgtacatg ccaaagtttc cacgccagtg catttacgaa
      301 aagcacgtac ttgctatgat catttagctg gcgaagttgc cgttaagatc tatgattccc
      361 tttgtcaaca gcaatggatc actgaaaatg gttcaatgat cacattaagt ggtattcaat
      421 attttcatga aatgggaatt gacgttcctt ccaaacattc acgtaaaatc tgttgtgcgt
      481 gtttagattg gagtgaacgc cgtttccatt taggtgggta cgttggagcc gcattatttt
      541 cgctttatga atctaaaggg tggttaactc gacatcttgg ttaccgtgaa gttaccatca
      601 cggaaaaagg ttatgctgct tttaagaccc actttcacat ttaagttgtt tttctaatcc
      661 gcatatgatc aattcaaggc cgaataagaa ggctggctct gcaccttggt gatcaaataa
      721 ttcgatagct tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc
      781 tttagcgact tgatgctctt gatcttccaa tacgcaacct aaagtaaaat gccccacagc
      841 gctgagtgca tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg
      901 attttcgaga gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc
      961 atcgcgatga cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg
     1021 ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc
     1081 taaggcgtcg agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac
     1141 acctagcttc tgggcgagtt tacgggttgt taaaccttcg attccgacct cattaagcag
     1201 ctctaatgcg ctgttaatca ctttactttt atctaatcta gacatcatta attcctaatt
     1261 tttgttgaca ctctatcatt gatagagtta ttttaccact ccctatcagt gatagagaaa
     1321 agtgaaatga atagttcgac aaagatcgca ttggtaatta cgttactcga tgccatgggg
     1381 attggcctta tcatgccagt cttgccaacg ttattacgtg aatttattgc ttcggaagat
     1441 atcgctaacc actttggcgt attgcttgca ctttatgcgt taatgcaggt tatctttgct
     1501 ccttggcttg gaaaaatgtc tgaccgattt ggtcggcgcc cagtgctgtt gttgtcatta
     1561 ataggcgcat cgctggatta cttattgctg gctttttcaa gtgcgctttg gatgctgtat
     1621 ttaggccgtt tgctttcagg gatcacagga gctactgggg ctgtcgcggc atcggtcatt
     1681 gccgatacca cctcagcttc tcaacgcgtg aagtggttcg gttggttagg ggcaagtttt
     1741 gggcttggtt taatagcggg gcctattatt ggtggttttg caggagagat ttcaccgcat
     1801 agtccctttt ttatcgctgc gttgctaaat attgtcgctt tccttgtggt tatgttttgg
     1861 ttccgtgaaa ccaaaaatac acgtgataat acagataccg aagtaggggt tgagacgcaa
     1921 tcgaattcgg tatacatcac tttatttaaa acgatgccca ttttgttgat tatttatttt
     1981 tcagcgcaat tgataggcca aattcccgca acggtgtggg tgctatttac cgaaaatcgt
     2041 tttggatgga atagcatgat ggttggcttt tcattagcgg gtcttggtct tttacactca
     2101 gtattccaag cctttgtggc aggaagaata gccactaaat ggggcgaaaa aacggcagta
     2161 ctgctcggat ttattgcaga tagtagtgca tttgcctttt tagcgtttat atctgaaggt
     2221 tggttagttt tccctgtttt aattttattg gctggtggtg ggatcgcttt acctgcatta
     2281 cagggagtga tgtctatcca aacaaagagt catcagcaag gtgctttaca gggattattg
     2341 gtgagcctta ccaatgcaac cggtgttatt ggcccattac tgtttgctgt tatttataat
     2401 cattcactac caatttggga tggctggatt tggattattg gtttagcgtt ttactgtatt
     2461 attatcctgc tatcgatgac cttcatgtta acccctcaag ctcaggggag taaacaggag
     2521 acaagtgctt agttatttcg tcaccaaatg atgttattcc gcgaaatata atgaccctct
     2581 tgataaccca agagggcatt ttttacgata aagaagattt agcttcaaat aaaacctatc
     2641 tattttat
//
LOCUS       DAJTOR010000459         1716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.guided.323, whole genome shotgun sequence.
ACCESSION   DAJTOR010000459 DAJTOR010000000
VERSION     DAJTOR010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.guided.323"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..752
                     /gene="blaOXA"
                     /locus_tag="OO249_004423"
     CDS             <1..752
                     /gene="blaOXA"
                     /locus_tag="OO249_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OXA-1 family class D beta-lactamase"
                     /protein_id="HCR5638445.1"
                     /translation="DISTVASPLFEGTEGCFLLYDASTNAEIAQFNKAKCATQMAPDS
                     TFKIALSLMAFDAEIIDQKTIFKWDKTPKGMEIWNSNHTPKTWMQFSVVWVSQEITQK
                     IGLNKIKNYLKDFDYGNQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNL
                     PVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVF
                     VSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            865..1656
                     /gene="aadA1"
                     /locus_tag="OO249_004424"
     CDS             865..1656
                     /gene="aadA1"
                     /locus_tag="OO249_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5638446.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          534 a          380 c          391 g          411 t
ORIGIN      
        1 cagatatctc tactgttgca tctccattat ttgaaggaac tgaaggttgt tttttacttt
       61 acgatgcatc cacaaacgct gaaattgctc aattcaataa agcaaagtgt gcaacgcaaa
      121 tggcaccaga ttcaactttc aagatcgcat tatcacttat ggcatttgat gcggaaataa
      181 tagatcagaa aaccatattc aaatgggata aaacccccaa aggaatggag atctggaaca
      241 gcaatcatac accaaagacg tggatgcaat tttctgttgt ttgggtttcg caagaaataa
      301 cccaaaaaat tggattaaat aaaatcaaga attatctcaa agattttgat tatggaaatc
      361 aagacttctc tggagataaa gaaagaaaca acggattaac agaagcatgg ctcgaaagta
      421 gcttaaaaat ttcaccagaa gaacaaattc aattcctgcg taaaattatt aatcacaatc
      481 tcccagttaa aaactcagcc atagaaaaca ccatagagaa catgtatcta caagatctgg
      541 ataatagtac aaaactgtat gggaaaactg gtgcaggatt cacagcaaat agaaccttac
      601 aaaacggatg gtttgaaggg tttattataa gcaaatcagg acataaatat gtttttgtgt
      661 ccgcacttac aggaaacttg gggtcgaatt taacatcaag cataaaagcc aagaaaaatg
      721 cgatcaccat tctaaacaca ctaaatttat aaaaaatcta atggcaaaat cgcccaaccc
      781 ttcaatcaag tcgggacggc caaaagcaag cttttggctc ccctcgctgg cgctcggcgc
      841 cccttatttc aaacgttaaa catcatgagg gaagtggtga tcgccgaagt atcgactcaa
      901 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
      961 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     1021 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     1081 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     1141 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     1201 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     1261 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     1321 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     1381 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     1441 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     1501 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     1561 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgttcactac
     1621 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga
     1681 cgccgcttcg cggcgcggct taactcaagc gttaga
//
LOCUS       DAJTOR010000460         1713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.guided.324, whole genome shotgun sequence.
ACCESSION   DAJTOR010000460 DAJTOR010000000
VERSION     DAJTOR010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.guided.324"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            <1..752
                     /gene="blaOXA"
                     /locus_tag="OO249_004425"
     CDS             <1..752
                     /gene="blaOXA"
                     /locus_tag="OO249_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OXA-1 family class D beta-lactamase"
                     /protein_id="HCR5638447.1"
                     /translation="DISTVASPLFEGTEGCFLLYDASTNAEIAQFNKAKCATQMAPDS
                     TFKIALSLMAFDAEIIDQKTIFKWDKTPKGMEIWNSNHTPKTWMQFSVVWVSQEITQK
                     IGLNKIKNYLKDFDYGNQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNL
                     PVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVF
                     VSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            865..1653
                     /gene="aadA1"
                     /locus_tag="OO249_004426"
     CDS             865..1653
                     /gene="aadA1"
                     /locus_tag="OO249_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5638448.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          531 a          380 c          391 g          411 t
ORIGIN      
        1 cagatatctc tactgttgca tctccattat ttgaaggaac tgaaggttgt tttttacttt
       61 acgatgcatc cacaaacgct gaaattgctc aattcaataa agcaaagtgt gcaacgcaaa
      121 tggcaccaga ttcaactttc aagatcgcat tatcacttat ggcatttgat gcggaaataa
      181 tagatcagaa aaccatattc aaatgggata aaacccccaa aggaatggag atctggaaca
      241 gcaatcatac accaaagacg tggatgcaat tttctgttgt ttgggtttcg caagaaataa
      301 cccaaaaaat tggattaaat aaaatcaaga attatctcaa agattttgat tatggaaatc
      361 aagacttctc tggagataaa gaaagaaaca acggattaac agaagcatgg ctcgaaagta
      421 gcttaaaaat ttcaccagaa gaacaaattc aattcctgcg taaaattatt aatcacaatc
      481 tcccagttaa aaactcagcc atagaaaaca ccatagagaa catgtatcta caagatctgg
      541 ataatagtac aaaactgtat gggaaaactg gtgcaggatt cacagcaaat agaaccttac
      601 aaaacggatg gtttgaaggg tttattataa gcaaatcagg acataaatat gtttttgtgt
      661 ccgcacttac aggaaacttg gggtcgaatt taacatcaag cataaaagcc aagaaaaatg
      721 cgatcaccat tctaaacaca ctaaatttat aaaaaatcta atggcaaaat cgcccaaccc
      781 ttcaatcaag tcgggacggc caaaagcaag cttttggctc ccctcgctgg cgctcggcgc
      841 cccttatttc aaacgttaaa catcatgagg gaagtggtga tcgccgaagt atcgactcaa
      901 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
      961 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     1021 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     1081 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     1141 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     1201 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     1261 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     1321 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     1381 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     1441 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     1501 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctaggca ggcttatctt
     1561 ggacaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg
     1621 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
     1681 cgcttcgcgg cgcggcttaa ctcaagcgtt aga
//
LOCUS       DAJTOR010000461         1309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 703280
            SAMEA8900985-rid15820563.guided.340, whole genome shotgun sequence.
ACCESSION   DAJTOR010000461 DAJTOR010000000
VERSION     DAJTOR010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900985
            Sequence Read Archive: ERR6005297
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478211.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478211.1
            Genome Coverage       :: 40x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2022 00:11:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,428
            CDSs (total)                      :: 4,349
            Genes (coding)                    :: 4,007
            CDSs (with protein)               :: 4,007
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 2 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 187 of 342
            Pseudo Genes (incomplete)         :: 85 of 342
            Pseudo Genes (internal stop)      :: 108 of 342
            Pseudo Genes (multiple problems)  :: 36 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900985-rid15820563.guided.340"
                     /serovar="S. flexneri 6"
                     /isolate="703280"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2010-04-07"
     gene            264..923
                     /locus_tag="OO249_004427"
     CDS             264..923
                     /locus_tag="OO249_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5638449.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..>1309)
                     /locus_tag="OO249_004428"
     CDS             complement(1124..>1309)
                     /locus_tag="OO249_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5638450.1"
                     /translation="AIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGGRINDHI
                     VNYYLHGESLSKLASGI"
BASE COUNT          349 a          288 c          310 g          362 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgc
//
